LOCUS       DASQOP010000001        13621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2416396, whole genome shotgun sequence.
ACCESSION   DASQOP010000001 DASQOP010000000
VERSION     DASQOP010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13621
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2416396"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..1851
                     /locus_tag="VE030_00005"
     CDS             154..1851
                     /locus_tag="VE030_00005"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZF21825.1"
                     /translation="MDHVSKKGAIKSMVLAMSLAGLAGWVQAGPVTDAMLAKDPGDSW
                     LHANGNWGGHRYSTLTQINDGNAKDLKVAWIFQTGGKTDAQNTPLFHDGLVFFAQDNK
                     IFALNADNGRIAWKFEHKLPEDWGGYNVGFITGKHRGMAIYGENLYFLSNDSKLHAIN
                     YKTGTVKYSKDFPGFEYPKDFAKAQDANGYATTVGPLAIPGAIIVPMNGTDFGGRPGY
                     VLGVNPDTGDMMWKANMIPGPGEPGADTWPPGSTEYGGAGPWITGSWDPDLKMYYTGT
                     ANAYEWNPKNRGGGEMDNVGAASIVAVNTTDGSVAWRYVGVPGDPWDFDIPQTPMVIT
                     IDGKKTVVQPNKTGYIHYLDAKTGKYLKATPFIDKITWIKGYDANGRPIGQVPLPKEG
                     GDPVEVYPSLLGGVNMYPNAYNPKTGNLYLAASETGMKYGFEEIKVISNVRHFGAYQE
                     FIWGDETDKAVNVKTGKEVWRDTRKGKPGYAGGMATTAGNVTFYTTQGGDFQVVNATT
                     GKILYHFDIGSTAKSGPITFMNKGKQMIVQAVGGLAGFGRDDALGLEMGGAVVAFSR"
     gene            2095..3798
                     /locus_tag="VE030_00010"
     CDS             2095..3798
                     /locus_tag="VE030_00010"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZF21826.1"
                     /translation="MDRISKNGVIKSMVLAMSLAGLAGWVQADPVTDAMLAKDAGDSW
                     LHANGNWAGHRYSTLNQINDSNAKDLKVAWMISPGGKTDAMATPLYHDGLVYFPQDNK
                     VFAFDADNGSVVWKYEHKLPEDWGGYNVLFFTGKHRGLAIYGDNVYFLSNDNKLHALD
                     AKTGKAKFVKDYQDKGFPYPKDFSKSQDANGYVSTVGPIAIPGAILLPLNATDTGGLP
                     GYVAAANPDTGEMMWKAPMIPGPGEPGHDTWPPGSHEFGGAGPWIVGSYDPELKIYFT
                     GTANAYEWNPKNRGGGEMDNVGAASIVAVNTTDGSVAWRYVGVPGDPWDFDIPQTPMV
                     ITIDGKKTIVQPNKTGFIHHLDAKTGKFLKADQFIDKMTWIKGYDKNGRPVGQTPLPK
                     EGGDPVEIWPALLGGVNMYPNAYNPKTGNLYLAAAESGMKYGFEDIKVISNVRHFGAY
                     QEFIWQDEVDKSVNVKTGKEAWRDTQRGKPGYAGGMMTTAGNLTVYTTQSGNFKVVNA
                     SSGKVLYSLNLGTAAKSGPITFNHKGKQMIVQALGGQAQFGRDDILKTEFGSVLVAFR
                     R"
     gene            3945..4352
                     /locus_tag="VE030_00015"
     CDS             3945..4352
                     /locus_tag="VE030_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZF21827.1"
                     /translation="MISAVFLAGVVLVVAGSFAERSARADEAAPEVNPLSGDQKAIRE
                     GKSWFRGICANCHGGRADGQGERGTGADLRKFNKGFKGFVGVVRKGREVPGRIQSMPA
                     WGAVLKEEQIYQIGAYLETLAIEGANWKEGVHN"
     gene            complement(4413..4796)
                     /locus_tag="VE030_00020"
     CDS             complement(4413..4796)
                     /locus_tag="VE030_00020"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZF21828.1"
                     /translation="MAIDGMNHFTVLTDNIDAARNFYVGILGLKEGYRPPLGFPGTWL
                     YAGSQPVLHVIAGRPLPASPAGVLDHMAFSASNLPDVTAKLKANGIKYDLRKQPDSGV
                     WQLFCFDPSGARVELDFAPGEKAPA"
     gene            complement(5010..6164)
                     /locus_tag="VE030_00025"
     CDS             complement(5010..6164)
                     /locus_tag="VE030_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HZF21829.1"
                     /translation="MASRPLLFTPISLRGVTLKNRVVISPMCQYSAKDGLANDWHFAH
                     LAKFAIGGAGLVFAEAAAVEADGRITYGDLGIWSDAHIAPLKRITDFVKSQGSVAAMQ
                     LAHAGRKASMQRPWDGNGPINESDRARGEDVWPVVAPSPIPMDSGWLTPHELTVNEIR
                     QIQDSWKKAALRALAAGFDVLEMHSAHGYLSHEFLSPLSNKRSDQYGGDFKSRTRFTL
                     ELAEVLRAVWPQDKPLFVRVSSVDGVEGGWTLDDTVALAKELKARGVDVLDCSSGGLM
                     GSATAARVKRSPGFQVPFAERVRKEAGMTTMAVGLILDPHQAQQVLQSGQADLIAVGR
                     QALYNPNWTLHAEVALGVQGEYESWPVQYGWWLDKRQHILAPEAKEKATA"
     gene            complement(6389..6850)
                     /gene="nikR"
                     /locus_tag="VE030_00030"
     CDS             complement(6389..6850)
                     /gene="nikR"
                     /locus_tag="VE030_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HZF21830.1"
                     /translation="MDRFTISLNEDLAKQFDELIHKKGYQNRSEAVRDMLRNELESAR
                     LEREESPYCVASLSYVYNHHARDLTERLTNLQHEHHQLVLSAMHVHLDHDNCLETVIL
                     RGATAEVSKFANQLMAEREVRHGRLNMVPVEMDEHRHNHINQYHVHTRPRT"
     gene            7148..9184
                     /locus_tag="VE030_00035"
     CDS             7148..9184
                     /locus_tag="VE030_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZF21831.1"
                     /translation="MTAGSYWAPALAQEKADIQAPEVEVLGTYQTGLGTSDAASAGTV
                     TARRVETRPALRPAELLEYVPGVIVTQHSGDGKANQYFLRGFNLDHGTDFAIDVAGMP
                     VNMPTHGHGQGYADLNFLIPELVSGIDYRKGPYFAEVGDFSAAGAASIRYTNSLPYTL
                     GSVTLGGGDYRRAVFAGSPNVGGGTLLYGLELMSNDGPWDNPQDFKKFNAALRYSRGG
                     EADGFSITGMAYQADWDSTDQIPLRAVQNGLIGRFGAIDPTDGGETSRYSLSYEQHYD
                     LGNNARAMLDLYAIRYKLNLFSNFTFFLDDTTNGDQFEQADNRTVLGLHPRLHFSNKL
                     GGMDNTMKIGLQVRRDHIDPVGLYHTVVRQRLSTTREDKVLETSAGVYVEDALQVTEW
                     FRAVPGIRVDRYEFEVDSSIPQNSGNKDDSIVSPKLSLVFGPWVKTEYFLNAGRGFHS
                     NDARGTTIKLDPNDPTLTTPVNPVHPLVRSTGAEIGVRTEIIPNLQSSISLWQLKLDS
                     ELLFVGDAGTTEPSRPSQRTGVEWTNHYRPASWLLVDFDLSVSRARFTDEDPAGDHIP
                     GSVEKVASFGVTVDSLGPWYGMLQYRYFGPRPLIEDDSVRSSSTQLTNLRVGYRIDPK
                     WRLHLDVFNLFDREDGDIEYFYKSKLQTEGAPVEDIHFHPVEPRTLRLTLTAHF"
     gene            complement(9400..10542)
                     /locus_tag="VE030_00040"
     CDS             complement(9400..10542)
                     /locus_tag="VE030_00040"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HZF21832.1"
                     /translation="MKFDFDTPIDRRGTGSTKWDKYKGRDVIPLWVADMDFACAPAVV
                     EALRCRVDHPIYGYSDPPVELAGTVVAYLQREYGWKIEPDWIVWLPGLVVGLNVVCRA
                     SGEPGDDVLTATPIYPPFLSAPVNSARNAVRVPMAEKNSVWRWDIAALERAITPKSRL
                     LLLCNPHNPVGRVFSRDELQAIAEVCERHDLTLCSDEIHCGLILDPDKRHVPVASLGE
                     NIARRTVTLMSASKTFNLPALGCAYAIASNSDLRARIRRAMAGIVHHVGMMGYIATLA
                     AYRDGKEWQLALLDYLRRNSDLVEREIGRIPALRIWHAEATYLSWIDARALPINNAQE
                     FLEEAGVGLYDGALFGAPGFLRLNFACPRPLLEQALERISRALRFL"
     gene            11036..11662
                     /locus_tag="VE030_00045"
     CDS             11036..11662
                     /locus_tag="VE030_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HZF21833.1"
                     /translation="MKAPNSEPRYIDALADLGTVRAYPKNTILVHEGDKSDQLYVVLS
                     GKLKVFLSDGEGREITIDILGPDQYFGEMALEGEPRSASIMTTEASRLSVLERSQFRQ
                     FFATNPEAAYWLYLALVRRARNLTKSVGGLALLDVYGRVARLLLDNASEESGCLVVGE
                     RITQQEIAKRVGASRETVSRILTDLREGNYITIENDRIKIQQHLPKRW"
     gene            complement(11628..>13621)
                     /locus_tag="VE030_00050"
     CDS             complement(11628..>13621)
                     /locus_tag="VE030_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF21834.1"
                     /translation="FIPPWLSRSHVTQLALTRLSRAQGASIVLRVAGGKPLPAELVTQ
                     IVDKTDGVPLFLEELTKAVLESNMIIDLGDRFDYSGSVDKLTIPNTLRDSLMARLDRL
                     IPVKEIAQIGACLGREFSHELVSAVSPMSEAQLTEALEKLTASELVFRRGSPPDSVYI
                     FKHALVQDAAYDSLLKTKRQELHAQIAEAIRERFPSKAEIEPELLAHHYTEAGLFGTA
                     IPYWQKAGELAQQRVALQEAIGHYQRGLELTSRLAPSSERDAWELQLRALLGMAWVEL
                     HGYTHPQVAASLEPALQLDQSLDRGDYAVRILWGLWVYRLCTGQIQESLVWAERLLAR
                     AEEVDSETMRIVGHWTACNSYFFLGELVQCVQHANALLARYDPVRDVGIADLVNHDPK
                     TIALMYRASAEWLLGFPDRAVATTELGIAHAGERQHPFDWCWARFFGTLQVYCPRGDL
                     DRAAALIVEMDNYATEQRLLFFKDIYIPMLRALCVAWAGRYAEGLVACERALQQWLGA
                     GSGIVVPYIKAAVAESAVRCNRQDEAIELLDEALAQIEWPEGHERYALAEVLRVKALA
                     VLAQGRPSAAEAIFQQALETARQQGAKSWELRAATSYALLLRDKGRVEDAISLVQPIY
                     HWFTEGHDTRDLRAARALLEELEQMPIDITSVSVSVAGS"
BASE COUNT         2853 a         3960 c         4203 g         2605 t
ORIGIN      
        1 gcttgtggat cggtcacggg gtgaggcacg tattcaacct gtggtttggt ccggcgcccg
       61 cccgtccact gcgggccgaa aagcgggttt tcggcatggg aaacgggagc ggtttaaatc
      121 ttctgacttg ataaccacta aaaggagaag gagatggacc acgtttcaaa aaaaggtgca
      181 atcaaaagca tggttctggc catgtcgctc gccggcctgg ccggttgggt acaggccggc
      241 ccggtgaccg acgcgatgct ggcgaaggat cctggggatt cctggctgca cgccaacggc
      301 aactgggggg ggcaccggta cagcacgctg acccagatca acgacggcaa cgccaaggat
      361 ctgaaagtcg cctggatatt ccagacgggt ggcaagaccg acgcgcagaa cactccgctg
      421 ttccatgatg gattggtgtt tttcgcgcag gacaacaaga tcttcgcgtt gaacgccgac
      481 aacggccgga tagcctggaa gttcgagcac aagctgcccg aggactgggg cggatacaac
      541 gttggtttca taaccggcaa gcatcgcggg atggcgattt acggagagaa cctctatttc
      601 ctgtcgaacg actccaagct gcacgcgatc aactacaaga ccgggacggt gaagtactcg
      661 aaggatttcc cgggctttga gtatccgaag gactttgcca aggcccagga cgccaatggc
      721 tacgcgacta cagtcggccc gcttgccatc cccggtgcga tcatcgtacc gatgaatggc
      781 acggacttcg gcgggcgtcc cggctacgtg ttgggcgtca atccggatac cggcgacatg
      841 atgtggaaag cgaacatgat tccagggccc ggagaacccg gggccgatac ctggccgccg
      901 ggaagcacgg agtatggcgg agccggtcca tggattaccg gctcgtggga tccggacctg
      961 aagatgtatt acaccggcac ggcgaacgcg tacgagtgga acccgaagaa ccggggcggc
     1021 ggcgagatgg ataacgtcgg cgcggccagc atcgtggcgg tcaacaccac tgacggcagc
     1081 gtggcatggc gctacgtcgg cgtgccgggc gatccctggg acttcgacat tccgcagacg
     1141 ccgatggtga tcaccatcga tgggaagaag accgtggtcc agcccaacaa gaccggctac
     1201 atccattatc tggatgcgaa gactggcaag tacctcaagg caacgccgtt catcgacaag
     1261 ataacctgga tcaagggtta cgacgccaac ggccggccga tcgggcaggt gccgctgccg
     1321 aaggaaggcg gcgaccctgt cgaggtctac cccagcctgc tgggtggggt gaacatgtat
     1381 cccaatgcct ataatccgaa gaccggcaat ctgtacttgg ctgcctccga aaccggaatg
     1441 aaatatggat tcgaggaaat caaagtcatc agcaacgtcc gtcacttcgg cgcgtaccag
     1501 gaattcatct ggggcgacga gaccgacaag gcagtgaacg tcaagaccgg caaagaggtc
     1561 tggcgggaca cccgcaaggg caagcccgga tatgcgggcg gcatggcgac gaccgctggc
     1621 aacgtgacgt tttatacgac ccaggggggc gacttccagg tggtcaacgc gacgaccggg
     1681 aaaatccttt atcacttcga cattggttca acggcgaaat ccggtccgat cacgttcatg
     1741 aacaaaggta aacagatgat cgtgcaggcg gtcggtggac ttgcgggatt cggacgtgac
     1801 gatgcgctgg ggctggagat gggaggggcg gtcgtggcgt tttcccgctg aagcaacaac
     1861 ggctttctgg cgcttgttcc aatgatgcag gcttcgggcg cgcaccggaa aacggtgcgc
     1921 gcaaccggat tgtgggtggg tcccccgggc aggatgcgcg ttcagaactc gggttctggt
     1981 ccagaacaac acgcgtgcct gccagtccac aacaggtgaa gaaaaaaaag tcttcgcctg
     2041 gggaatgaca gtggttacat tcttagaact tacataaaca gaggagagaa gcagatggac
     2101 cgcatttcga aaaacggggt aataaaaagc atggtgctgg ccatgtcgct cgccggcctg
     2161 gccggatggg tacaggccga cccggtgacc gacgcaatgt tggcgaagga cgccggggat
     2221 tcctggctgc atgccaacgg caactgggcc ggacaccggt acagcacgct gaaccagatc
     2281 aacgacagca atgcaaaaga cctgaaagtt gcgtggatga tttcgcccgg cggcaagacc
     2341 gatgcgatgg ccacgccgct gtatcacgat ggcctggtct acttcccgca agacaacaag
     2401 gtgtttgcct tcgacgccga caacggcagc gtggtgtgga agtacgagca caagctgcct
     2461 gaggactggg gcgggtacaa cgtcctgttc ttcaccggca agcatcgggg actggccatc
     2521 tacggggaca acgtctactt cctgtccaac gacaacaaac tgcatgcgct cgatgccaag
     2581 accgggaagg caaaattcgt caaggattac caggacaaag gcttcccgta tccgaaggac
     2641 ttttccaagt cgcaggacgc aaacggttat gtcagcaccg tgggcccgat cgctattccg
     2701 ggcgccatcc tgctgccgtt gaacgcaacc gataccggcg gcctgcccgg gtacgtcgct
     2761 gccgccaatc cggataccgg cgaaatgatg tggaaggcgc cgatgattcc cggacccggt
     2821 gagcctggcc acgacacgtg gcctcccgga agccatgaat tcggcggtgc aggtccctgg
     2881 atcgtcggct catacgatcc cgaactgaag atatatttca ccggcaccgc caacgcctac
     2941 gagtggaacc cgaagaaccg gggcggcggc gagatggata acgtcggcgc ggccagcatc
     3001 gtggcggtca acaccactga cggcagcgtg gcatggcgct acgtcggcgt gccaggcgat
     3061 ccgtgggact tcgacattcc gcagacgccg atggtgatca ctatcgacgg taagaagacc
     3121 atcgtccagc ccaacaagac cggattcatc catcacctgg atgcgaagac cggcaagttc
     3181 ctcaaggccg accagttcat cgacaagatg acctggatca agggatacga caagaatggc
     3241 cgtccagtcg gacagactcc gttgcccaag gaaggcggcg atccggtgga gatatggcct
     3301 gcactgctgg gcggcgtgaa catgtatccg aacgcctata acccgaaaac gggcaacctg
     3361 tatctggccg ctgccgagtc cggaatgaaa tatggattcg aggacatcaa ggtcatcagc
     3421 aacgtccgtc acttcggtgc ctaccaggaa ttcatctggc aggacgaggt ggacaagtcg
     3481 gtcaacgtca agaccggaaa ggaagcctgg cgcgacacgc agcggggcaa gccgggctat
     3541 gccggaggaa tgatgacgac cgcaggtaac cttaccgtct ataccaccca aagcgggaat
     3601 ttcaaggtgg tcaatgcctc cagcggcaag gtgctgtact cgttgaatct cggcaccgcc
     3661 gccaagtcgg gcccgatcac gttcaatcac aagggcaaac agatgatcgt gcaggcgcta
     3721 ggcggacagg cccagttcgg tcgcgatgac attctcaaga ccgagtttgg cagcgtgctt
     3781 gttgccttca gacgttaaga atcacagggc tctgtaaaag tccttcgatg acgtagggaa
     3841 aggcgcgcac cgggtttctg cggtgcgcgc cattttttgc cccgaggggc cagtcaaatc
     3901 acaagaaaaa aggaacagga aagatggcac gaattgcgac caaaatgata tcggcggtct
     3961 ttctcgccgg tgtggtgctg gtagttgcag gtagtttcgc ggaacgcagc gcgcgcgcgg
     4021 atgaggcggc tccggaagtg aatccgctgt cgggtgatca gaaagcgatc cgggagggca
     4081 agtcctggtt ccgcgggata tgcgcgaact gccatggagg aagggcggac ggccagggcg
     4141 agcgtggcac gggcgccgac ctgcgcaagt tcaacaaggg cttcaagggt tttgtggggg
     4201 tggtaaggaa gggccgcgag gtgccgggcc ggatccagag catgccggcc tggggtgcgg
     4261 tgttgaaaga ggagcagatc taccagatcg gcgcctacct cgaaacgctt gctattgaag
     4321 gcgccaactg gaaggaaggc gttcacaact aacggggcgc gcggccaggt gcccctcagc
     4381 gaatcgaccg gcagcgatcc cggccagagc tgtcaggccg gcgctttttc ccccggcgcg
     4441 aagtccagtt cgactcttgc gccgctgggg tcgaagcaga acaactgcca tacgccggag
     4501 tccggctgtt tcctcaagtc atatttgatg ccgttcgcct tcaacttcgc cgtgacatcg
     4561 ggaagattgg aggcagagaa cgccatgtgg tcgagcacgc cagcgggact tgccggcagt
     4621 ggcctgccag cgatgacatg caggaccggc tggcttccgg cgtacagcca ggtcccggga
     4681 aatcccagag gcggccgata accttccttc agaccgagaa tgccaacgta gaagttgcgg
     4741 gcggcatcga tgttgtcggt gagcaccgtg aagtgattca ttccgtcaat ggccatggga
     4801 actccgtgag aaagccgtga cgatcgtgac gacgtgacgg cgtgacgacg taaaggcacg
     4861 gaagatcaac atcaaatggt tttctgaata tctcaagcgt tgcaccctgg ttactccgca
     4921 gcacgctgcg ctttgcatag gattcgcgtc gcgaccgcga cgttcttgtc ctggttttgc
     4981 cctgtcacgc cgtcacgtcg tcacggcctt tatgccgtcg ccttttcctt cgcctcaggc
     5041 gccagtatgt gctggcgctt gtccagccac cagccgtact gaaccggcca cgattcgtat
     5101 tctccttgca cgcccagcgc gacttcggcg tgcagcgtcc agttcgggtt gtataacgcc
     5161 tggcgtccca ctgcgatcag gtcggcctgg cccgattgca gcacctgctg tgcctgatgc
     5221 ggatccagga tcaggcccac cgccatggtc gtcatgccgg cttctttcct cacgcgctcc
     5281 gcgaacggca cctggaaacc ggggctgcgc tttacccgcg ccgcggtagc cgaacccatc
     5341 agtccgccag aggagcagtc gagcacatcc acgccgcgcg ccttgagttc cttggccagc
     5401 gccacggtgt cgtccagcgt ccagccgcct tcgacgccgt ccaccgagga tacgcgtacg
     5461 aacagcggtt tgtcctgcgg ccagacggcg cgcaggactt ccgccagttc cagcgtgaaa
     5521 cgcgtgcggc tcttgaagtc tccgccgtac tgatcgctgc gcttgttgga aagcggggag
     5581 aggaactcgt ggctgagata gccgtgcgcg gaatgcatct cgagcacgtc gaaccctgct
     5641 gcgagcgcgc gtaacgctgc ctttttccag gaatcctgaa tctggcgaat ctcattcacg
     5701 gtgagctcgt gtggcgtgag ccagccgctg tccatcggaa tcgggctggg cgcgaccacc
     5761 ggccagacgt cttcgccgcg tgcgcggtcg ctttcgttga tcggtccgtt gccgtcccag
     5821 gggcgttgca tggacgcctt gcggcctgcg tgcgcgagct gcattgccgc cacggaaccc
     5881 tgcgacttga cgaaatcggt gatgcgcttc aatggcgcga tatgggcgtc ggaccagatg
     5941 ccgaggtcgc cgtaggtaat gcggccgtcg gcttctacgg cagcggcttc ggcgaagacg
     6001 agtccggcgc cgcctatcgc aaacttggcg aggtgagcga agtgccagtc gttggccagt
     6061 ccgtccttcg cggagtattg gcacatcggc gagatgacca cgcggttctt gagcgtcacc
     6121 ccgcgcagcg atatcggcgt gaacaaaaga ggtcttgatg ccattcagtt tcctttcagt
     6181 tgaatcgact tgagcagcac tatatttaaa gacacactct caccgcagag gacgcccccc
     6241 tgtccccctc cctctccccc taaaaccggg tggagagggt gatgaaaacc cgctaatccg
     6301 aaatcgtatg gtttttcggc gctctttcga gcttgctgga gagggcgggg ggtgaggtga
     6361 tttcctccgc ggttcgcgcg tgaatctcct aagtacgggg ccgggtgtgg acgtggtatt
     6421 gattgatatg gttgtggcga tgctcgtcca tttccaccgg caccatattg agccgcccgt
     6481 gacggacctc gcgctcggcc atcaactggt tggcgaactt gctaacttcg gcggtggcgc
     6541 cgcgcaggat gacggtctcc agacaattgt cgtggtcgag gtgcacgtgc atcgccgaca
     6601 agaccaattg atggtgttcg tgctgaagat tggtgagtcg ctcggtcagg tcgcgggcgt
     6661 ggtgattgta cacataagat aacgaggcca cgcagtaggg cgattcctcg cgctccagac
     6721 gtgccgactc gagttcgttc ctgagcatgt cgcgcacggc ttccgaacga ttctggtagc
     6781 ccttcttgtg gatcaattcg tcgaattgtt ttgccagatc ttcgttcagc gaaatcgtga
     6841 atcggtccat cgcaggttcc caggattgtg actcgtggga tgttaccgaa aatacgtagg
     6901 aaaggcgcga tgtacacaag aggccgtggg ggtctcccgg aaagctttac ttttgtttcg
     6961 atggccctgt attaagaaga atgagtaaaa ttacttactt cctatgttac aataaaacaa
     7021 gggcttggat gacgatcctg gaacgaggtc gggccaaaga cccggggaga caagaaaaat
     7081 gaaggcggta acaagagcaa aaacgggcgc tgcaagaatt ctccagtttt ttctgttttc
     7141 gggattcatg actgccggtt cttactgggc ccctgcgctg gcgcaggaaa aggcagacat
     7201 ccaggcgccg gaagtcgaag tgctcggtac ttaccagacc gggttgggaa cctcggatgc
     7261 ggccagcgca ggcacggtca ccgcgcgcag agtcgaaacc cggccggccc tgcgtccggc
     7321 cgaactgctg gagtacgttc ccggagtgat cgtcacccag cacagcggtg acggtaaagc
     7381 caatcagtac ttcctgcgcg gcttcaacct cgatcacggc accgacttcg cgatcgacgt
     7441 cgccggcatg ccggtgaaca tgcccacaca cgggcacgga caaggctatg ccgacctgaa
     7501 cttcctgatt ccggaactgg tgtccggcat cgactatcgc aaggggcctt acttcgccga
     7561 ggtcggtgac ttcagcgcgg ccggcgccgc cagcatccgc tacaccaatt cgctgcctta
     7621 cacgctcggt tcggtgacgc tcggaggggg ggactaccgg cgcgcggtgt tcgccggctc
     7681 cccgaacgtc ggcggtggca cgctgctgta cgggctcgaa ctgatgagca acgacggacc
     7741 ctgggacaac cctcaggact tcaagaagtt caacgcagcg ttgcgctaca gccgcggcgg
     7801 cgaagcggac ggattctcca tcaccgggat ggcttaccag gccgactggg attccaccga
     7861 ccagattccg ctgcgggcgg tgcagaacgg tctgatcggc cgcttcggcg caatcgaccc
     7921 gaccgacggc ggcgagactt cgcgctattc gctgtcctac gagcagcact acgatctcgg
     7981 aaacaacgcg cgggcgatgc tggatctcta tgcgatccgt tacaagctga acctgttctc
     8041 gaacttcacc tttttccttg atgacacgac caacggcgat cagttcgagc aggccgataa
     8101 tcgcaccgtc ctcggcttgc acccgcgcct gcacttcagc aacaagctcg gcggaatgga
     8161 caacacaatg aagatcggcc tgcaagttcg ccgcgaccac atcgacccgg tgggccttta
     8221 ccacacggtt gtgcgtcagc gtttgagcac cacgcgcgaa gacaaggtgc tggagaccag
     8281 cgccggggtt tatgtcgagg atgcgctgca ggtcaccgaa tggttccgcg cagtaccggg
     8341 aatccgcgtg gaccgctacg agttcgaggt ggacagcagc attcctcaga actccggcaa
     8401 taaggacgac tcgatcgtgt cgccgaagtt aagcctggtg ttcggccctt gggtgaagac
     8461 cgaatacttc ctcaatgcgg gccggggttt ccacagcaac gacgcccgcg gcacgacaat
     8521 caagctcgat ccgaacgatc cgacattgac tacgccggta aatcccgtac atccgttggt
     8581 gcgctccacc ggcgcggaaa tcggcgtgcg caccgaaatc attcccaatt tgcagtcgtc
     8641 gatatcgctg tggcaattga agctggattc ggaactgctg ttcgtcggcg atgccgggac
     8701 caccgagccg agccggccta gccaacgcac cggcgttgag tggaccaacc attaccggcc
     8761 cgcatcatgg ctgctggtgg acttcgatct gagtgtgtca cgcgcgcgct tcaccgacga
     8821 agatccggcc ggcgaccaca tcccgggttc tgtcgagaaa gtcgcgtcct tcggggttac
     8881 cgtggattcg ctggggcctt ggtacggaat gctgcaatac cgctacttcg ggccgcgtcc
     8941 tctgatcgag gatgattcgg tgaggtcaag ttccacgcaa ctcaccaacc tgcgcgtcgg
     9001 ataccggatc gacccgaagt ggcgcttgca tctggacgtg ttcaacttgt tcgatcgcga
     9061 ggacggcgac atcgagtatt tctacaagtc gaaactgcag accgaaggcg cgccggtgga
     9121 ggacattcat ttccatcctg tcgagccgcg caccttgcgc ctgactctga ccgcgcattt
     9181 ctgaagaaag cttttcacca cagagacaca gagacacgga ggagaaacaa gaacaaggaa
     9241 agaaaatgta gattagaagg ggtgaccgta cgcgaacctg caccgccatc ttgtagcgat
     9301 cgatatctgc aaacgaacga ctttaacctc ggtatctttt ttctcttggt tttcctctgc
     9361 gtctctgtgt ctctgtcgtg aatggatttg gtacgaaagt catagaaaac gaagggcgcg
     9421 agagatgcgc tcgagcgcct gctcgagcag tgggcgcgga caggcgaagt tgaggcgcag
     9481 aaagcccggc gcaccgaaca gcgcgccgtc gtagagaccg acgccggcct cttcgagaaa
     9541 ctcctgcgcg ttgttgatcg gcagcgcgcg cgcatcgatc cacgacaaat aggtcgcctc
     9601 cgcatgccag atgcgcagcg ccggaatgcg gcctatttcc cgctcaacga ggtcgctgtt
     9661 gcggcgcagg tagtcgagca gcgcgagctg ccattccttg ccgtcccggt atgcagccaa
     9721 tgtcgcgatg tatcccatca tgccgacgtg gtggacgatc cccgccatag ctctcctgat
     9781 tcttgcacgc aagtccgaat tcgacgcgat tgcgtaggcg caacccagcg cgggcaggtt
     9841 gaaggtcttg cttgcggaca tcagcgtcac cgtgcggcgc gcgatgtttt cacccagcga
     9901 cgccacggga acatggcgct tgtccggatc gaggatcagg ccgcagtgga tttcgtcgga
     9961 gcagagtgtg aggtcatggc gttcgcacac ttcggcaatc gcctgcaatt cgtcgcgcga
    10021 gaacacgcgg ccgaccggat tgtgcggatt gcagagcagc agcaggcgtg acttcggcgt
    10081 gatggcgcgc tcgagcgccg cgatgtccca gcgccagacg ctattctttt ccgccatggg
    10141 cacgcgcacc gcgttgcgcg cggaatttac cggggcggac aggaagggcg gatagatcgg
    10201 tgtggcggta aggacgtcgt cgcccggctc gccggaggcg cgacatacga cattgaggcc
    10261 gaccaccagt cccggcagcc agacgatcca gtccggttcg atcttccagc cgtattcgcg
    10321 ctgcaagtag gcgacgactg tgcctgccag ttcgacgggc ggatcggagt acccgtaaat
    10381 cggatgatcg acacggcaac gcaacgcttc gaccaccgcc ggcgcgcagg cgaagtccat
    10441 gtccgccacc cacagcggaa tcacgtcccg gcccttgtac ttatcccatt tggtgctgcc
    10501 cgtaccgcgg cggtctatgg gcgtgtcgaa atcaaacttc aatgctctct cccagagtga
    10561 ccggtattag agcacagggc ctagcgaacg gagacgggag agcgaaacgc atggaccgga
    10621 cgccggaggc gccatggatc aaaagcgttg acgcaaagga aatcggaacg gaaacgttgg
    10681 gagtggttta acgtttcctc aggcactctt gatcccgata ttctcgctgc gttgagaagg
    10741 aagtagaaag gatacaaaag gcagacggcc cgtcaatccc gacattgtct ccggaccgca
    10801 aagctctgtt tcctgatcct ggttttccct ggcgttcttt gcgtccctgg cggtgatcag
    10861 gtttttcggg tcagtggaaa tcgcgcgagt gagtgacaag gttgccgagc agtgccaaat
    10921 agtcgctgag gcggctggcg atccgttgga aagcaggatt cacaagctcc cggcttgcgt
    10981 ccgccacgca tggggtgcag gataaagcgc atggcaaatc cgcattcccg ttgagatgaa
    11041 ggctcccaat agcgagccgc gttacatcga cgcgctcgca gaccttggaa ccgtgagggc
    11101 ttaccccaag aacacaattc tggttcacga aggcgacaag agcgaccagt tgtatgtcgt
    11161 gctatccggg aagttgaagg tctttctctc ggacggcgaa ggaagagaaa tcaccatcga
    11221 tatcctaggt ccggaccaat atttcggcga aatggcgttg gagggtgagc cacgttccgc
    11281 atcgattatg acgacggagg cgtcgaggtt gtcggtactc gaacgctcgc agttcaggca
    11341 gttctttgcc acaaacccgg aggcggccta ctggctttac ctcgcgttgg tccgcagggc
    11401 gagaaatcta actaagagcg tcgggggtct cgcacttctt gatgtctatg ggagagtcgc
    11461 tcgattgttg ctggacaatg ctagcgaaga gtcaggttgc cttgtggtag gagagcgtat
    11521 cactcagcag gaaattgcaa agcgcgtggg agcttcgcgc gagacggtgt cgaggatcct
    11581 caccgacctt cgcgagggca actacattac gatcgagaat gatcgaatca agatccagca
    11641 acacttaccg aaacgctggt aatgtcgatc ggcatctgct cgagttcttc cagcaacgcc
    11701 cttgctgcgc gcaggtcacg cgtatcgtgg ccttcggtga accagtggta gatcggctgt
    11761 accaaagaga tcgcatcttc aactctgcct ttgtccctca atagcaaggc atagctggtt
    11821 gcagcgcgta gttcccagga tttcgcgcct tgctgccgtg ccgtttctaa tgcttgctgg
    11881 aatatcgctt cggctgcgct tgggcggccc tgcgccagca cggccaatgc cttcacccgc
    11941 aggacttcgg caagggcata acgctcgtgt ccctccggcc attctatctg cgccagcgcc
    12001 tcgtccagca attcaatcgc ttcgtcctgc cgattgcatc gcacggcact ttcagctact
    12061 gcagccttta tgtatggcac cacgatcccg gatccagcgc ctaaccactg ctgaagcgcc
    12121 cgttcgcacg cgaccaaccc ctcggcgtag cgaccggccc acgcgacgca cagcgcgcgc
    12181 agcatcggaa tatagatgtc cttgaagaaa agcaagcgtt gctccgtcgc atagttgtcc
    12241 atctccacga tcagcgccgc cgcacgatcg aggtcgccgc gtgggcaata gacttgcagc
    12301 gtgccgaaaa agcgcgccca gcaccagtcg aacgggtgct gccgttcccc tgcgtgagcg
    12361 atgcccaatt ccgtcgtggc aaccgcccga tcaggaaagc ccaataacca ttcggcgctt
    12421 gccctgtaca tgagggcaat ggttttcggg tcatggttga ccaggtcggc gataccgaca
    12481 tctcgcaccg ggtcgtagcg ggcgaggagc gcgttggcat gttgcacgca ctggaccagt
    12541 tcaccgagga agaagtagga gttgcaggcg gtccagtggc cgacgatgcg catggtttcg
    12601 ctgtcgacct cctcggccct ggccagcagc cgctccgccc acacgagcga ctcctggatc
    12661 tgaccagtgc agagacggta tacccacaat ccccacagga tccgaactgc gtagtcgcct
    12721 cggtctagcg actggtccag ttgcagggca ggctcgagac tagcggcgac ctgtgggtgg
    12781 gtatacccgt gcagctcgac ccacgccatg ccgagcagtg cccgcagttg cagttcccag
    12841 gcatcgcgct cgctcgaagg agcgagcctc gaggtgagct ccaggccgcg ctggtaatgt
    12901 ccgatcgctt cttgaagtgc gacgcgctgc tgggccagtt cgccggcttt ctgccagtac
    12961 gggatcgctg ttccaaacag cccggcttcg gtgtagtggt gcgccagcag ttcgggttcg
    13021 atttcggcct tcgaggggaa gcgctcgcgg atggcctctg cgatctgcgc gtgcagctcc
    13081 tgacgcttgg tctttagcag cgagtcgtag gcggcatcct gcaccagtgc atgcttgaag
    13141 atatacaccg aatcgggtgg gctgccgcgc ctgaagacca gctctgacgc ggtgagcttc
    13201 tccagcgctt cggtcagttg cgcctcgctc atcggcgaga ccgcggacac cagctcatgg
    13261 ctgaactcgc gccccaggca ggcgccgatt tgggcgatct ccttgaccgg tatcagccgg
    13321 tccagccgcg ccatgagcga atcgcgtagt gtgttgggaa tggtcagctt atcgaccgac
    13381 cccgagtagt cgaagcggtc gcccaagtcg atgatcatgt tcgattcgag caccgccttg
    13441 gtcagctcct ccagaaacag cggcaccccg tcagtcttgt ccacgatctg ggtgaccagt
    13501 tcggcgggta gcggtttccc gccggcaacc cgcagcacga tgcttgcccc ctgcgcgcgg
    13561 cttagccgcg tgagcgcaag ctgcgtgacg tgcgaacgcg agagccacgg cgggatgaac
    13621 t
//
LOCUS       DASQOP010000002         2600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_201965, whole genome shotgun sequence.
ACCESSION   DASQOP010000002 DASQOP010000000
VERSION     DASQOP010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201965"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="VE030_00055"
     CDS             complement(<1..532)
                     /locus_tag="VE030_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010457773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HZF21835.1"
                     /translation="MAPAPAFSTVLLVRTVVAWIVTLLLFFPLGWLVLTAFKTELQAI
                     SVPPLLFFTPTLDNFVEVERRSDYLLYAQNSLITSVVSTLLGLAIAAPAAYSMAFFRS
                     KWTRDVLMWMLSTKMMPAVGALVPIYVLAQTAGLLDTRLALIMVFMLSNLPIMVWMLY
                     SSLRDIPHEILEAARMD"
     gene            complement(556..1413)
                     /locus_tag="VE030_00060"
     CDS             complement(556..1413)
                     /locus_tag="VE030_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HZF21836.1"
                     /translation="MTRFLPRALLSPAVITLFLWMIVPLVMTIYFSLIRYNLMQPEEH
                     GFIGLANYHFFITDPSFGPAIINTLLLLGSVIVITVIGGVALALLIDDPFPGRAVVRV
                     LLISPFFVMPTVNALLWKHMMMNPIYGVFAQVWIFFGMQPVDWMTDWPLFSVILMVAW
                     QWLPFATLIFVTALQSMNREQLEAARMDGARYLQQLRYLYVPHLGRSIAVVIMIEMIF
                     LMSVFAEIYTTTGGGPGDASTNVAFLIFKQALLNFDAGVASAGALFAVVLANIVAIFL
                     IRLIGKNLD"
     gene            complement(1482..>2600)
                     /locus_tag="VE030_00065"
     CDS             complement(1482..>2600)
                     /locus_tag="VE030_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010457770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HZF21837.1"
                     /translation="VLRQRVTTDIATKGGQFDVMTIGMYETPIWGKKGWLQELKPDAA
                     YDVDDLLPAMRQGLSVDGKLFAAPFYGESSMLMYRKDLADKAGVKLPERPTWAQVKEL
                     AAKIHNPPAVYGICLRGKPGWGDNMAFLTTLVNTNGGQWFDMSWKPQLESKPWKDAIK
                     FYVDLLNKYGPPGSSANSFNEILALYNEGKCGMWVDATIAASFISDPKQSKVADKVAF
                     AQAPVAVTPKGANWLWAWALAIPAGSKNQEAAQKFIAWATSKDYVKLVGKEAGWGSVP
                     TGTRKSTYTTPEFLKAAKFADAEKKAIDSANPNDSTLPKSPYVGVQFAAIPEFQAIGI
                     AVGQQMSAALAGKVTVDEALKSSQNSADREMRKAGYYK"
BASE COUNT          523 a          799 c          793 g          485 t
ORIGIN      
        1 cgtccatacg ggccgcttcg agaatctcgt gcgggatgtc gcgcaggctc gagtacagca
       61 tccataccat gatgggcagg ttgctgagca tgaacaccat gatcagcgcc aggcgcgtgt
      121 ccagcagccc cgccgtctgc gccagcacgt agatcggcac cagcgcgccc accgccggca
      181 tcatcttggt ggacagcatc cacatcagca catcccgcgt ccacttgctg cggaagaacg
      241 ccatcgagta cgccgcgggc gccgctatgg ccagcccgag cagcgttgaa accacgctgg
      301 tgatcagcga attctgcgcg tagagcaaat aatcgctgcg ccgctccacc tctacgaagt
      361 tgtccagcgt tggcgtgaag aacagcagcg gaggcaccga gatcgcctgc aactcggtct
      421 taaaggcggt cagcaccagc cagccgagcg gaaagaagag caggagggtc acgatccatg
      481 cgacgacggt acgcacgagc aggacggtgg aaaaggcggg cgcgggagcc atgggtttgt
      541 caatccaggg gttcgtcaat ccaggttctt gccgatgagg cggatgagaa atatcgccac
      601 gatattggcg aggacgacgg caaagagggc gccggccgac gccacgccgg catcgaagtt
      661 gagcaacgcc tgtttgaaga tcaggaatgc gacgttggtg ctggcatcgc ccgggcctcc
      721 gccggtggtc gtgtagattt cggcgaaaac gctcatgagg aaaatcatct cgatcatgat
      781 cacgaccgcg atcgagcgcc cgaggtgcgg gacatacaga tagcgcagct gttggaggta
      841 gcgtgcgcca tccatgcgcg cggcttccag ctgctcgcgg ttcatcgact gcagcgcagt
      901 cacgaagata agcgtggcaa acggcaacca ctgccaggca accatcaaga tcacggagaa
      961 cagcggccag tccgtcatcc aatccacggg ttgcatcccg aagaaaatcc agacctgcgc
     1021 gaacaccccg tagatcgggt tcatcatcat gtgcttccac agcagcgcgt tcaccgtggg
     1081 catcacgaag aagggcgaga tcagcagtac gcgcaccacg gcgcgccccg ggaatggatc
     1141 gtcgatgaga agggccagcg caaccccgcc gatcacggtg atgacgatga cgctgccgag
     1201 cagaagcaac gtgttgatta tggccggccc gaacgacggg tcggtgatga agaaatgata
     1261 gttggccagc ccgatgaagc catgctcttc cggctgcatg aggttgtagc ggatgagcga
     1321 aaagtagatc gtcatcacca gcggcacgat catccacagg aagagcgtga tcactgcggg
     1381 cgagagcagc gctcggggaa gaaatcgggt catcgtttca gtgcctggta gtcgggccgg
     1441 cgccggccgg cccgaacgtc agttgacaat tgcaccgggg attacttgta gtatcccgcc
     1501 tttctcatct cgcggtctgc cgaattctgg gagctcttca gcgcctcatc gaccgtgact
     1561 ttcccggcca aagccgcgct catttgctgc ccgaccgcaa taccgatggc ctgaaactcc
     1621 gggatcgcgg caaactggac gccgacgtat ggcgatttcg gcagcgtgct gtcgttggga
     1681 ttggcggagt cgatcgcctt cttctcggcg tcggcgaatt tggctgcctt caagaactcc
     1741 ggcgttgtat aggtgctttt gcgcgttccc gttggcacac tgccccagcc cgcttccttg
     1801 cccaccagct tcacgtagtc cttcgacgtg gcccaagcga tgaacttctg cgccgcctcc
     1861 tggttcttgg aaccggccgg aatcgccagc gcccaagccc acagccagtt ggcccccttg
     1921 ggcgttaccg cgacaggcgc ctgtgcgaaa gccactttgt cggcgacctt gctctgcttc
     1981 gggtcgctga taaaggaggc ggcgatcgtg gcatcgaccc acatgccgca cttgccctcg
     2041 ttgtacagcg ccaggatctc gttgaagctg ttggccgacg agccgggcgg gccgtacttg
     2101 ttcagcagat ccacgtagaa cttgatggcg tctttccagg gcttggattc gagctgcggc
     2161 ttccagctca tgtcgaacca ctgtcccccg ttggtgttga ccagcgtagt gaggaacgcc
     2221 atgttgtcgc cccagcccgg cttgccgcgc aggcaaatcc cgtagacggc gggcggattg
     2281 tggatcttcg ctgccagctc tttgacctgc gcccaggtcg gccgctccgg aagctttaca
     2341 ccggctttat cggcgaggtc cttgcgatac atcagcatcg aactctcgcc atagaagggt
     2401 gccgcgaaca gcttgccgtc gaccgacagg ccctgccgca tcgccggcag gagatcgtcc
     2461 acgtcatacg cggcatcggg cttgagttcc tgaagccaac ccttcttgcc ccagatcggc
     2521 gtttcgtaca taccgatggt catgacgtcg aactggccac ccttcgtggc gatgtcggtt
     2581 gtcacccgct ggcgcagcac
//
LOCUS       DASQOP010000003         5846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1209128, whole genome shotgun sequence.
ACCESSION   DASQOP010000003 DASQOP010000000
VERSION     DASQOP010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5846
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209128"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VE030_00070"
     CDS             complement(<1..624)
                     /locus_tag="VE030_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HZF21838.1"
                     /translation="MAKDILCAFGVDVDAVAGWLGSYGGEDSPDDISRGLFAGEVGTP
                     RLVNLFKRLGVKTTWFIPGHSIETFPEQTAMVVEAGHEIGIHGYTHENPISMTREQEE
                     AVLDKCIDLVTKVSGRRPTGYVAPWWEFSTVTNELLLKRGIKYDHSLMHKDFEPYYVR
                     VGDSWTLIDYSKKPQEWMKPLVRGVETDLIEIPGSWYLDDLPPMMFIK"
     gene            768..1562
                     /locus_tag="VE030_00075"
     CDS             768..1562
                     /locus_tag="VE030_00075"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZF21839.1"
                     /translation="MPETFSKKIAAESVKRTTLSEAAHQAIRRNIITGVLAPGSKLAV
                     AALAQALKLSATPINEALAGLEREGLVTYSPHRGYFVRTVTPEDIEETYSVREAFELL
                     AVRNAAKNADKTVIGQLREILQKSRQSLRETDTSRFSDLDLEFHRVIWNSLNNSLATR
                     VGELIGVQIRLLVATTARAPGRFRGAFEEHNDIYQAIRNRDPTRAESSMRTHLHNAKT
                     ALEHAVFEEAGTGSNVSAIGASKTKPGAKRGLAREVSAQSVPKQRT"
     gene            complement(1665..2249)
                     /locus_tag="VE030_00080"
     CDS             complement(1665..2249)
                     /locus_tag="VE030_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF21840.1"
                     /translation="MNKPKDLLEAYLEAKDLDRPSLILECYAPNAVLTYSIATDTISF
                     PSKVLGADAIAQTLVRDFRKKFDRCKTYYVSDSIVDRSLRIDFMPWLVIMREVSNSAL
                     RVGKGYYRWQFESDETRMRVCAMHIHIERMDIIERRDGKTLDALQSELPYPWLPRATL
                     LETFERLMKQAPGFEFLDVFKVPVGKQERTERPA"
     gene            complement(2344..3459)
                     /locus_tag="VE030_00085"
     CDS             complement(2344..3459)
                     /locus_tag="VE030_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HZF21841.1"
                     /translation="MLTLAVAIGIGRFAFTPILPMMQKDAGLSLAAAGWLASANYVGY
                     FIGALSAVWIRARSAAIVRGSLVAIALLTAGMGVTDNPLAWLTLRALAGIASAWALVF
                     GSAWLLPRLAAMGSHRFGGVVFGGVGLGTALAGGLCLVFLQLAWSADRAWIAMGSVAL
                     LFTVMIWQRYTGAAAPVAAGTAAVAPLHSGGHMRLIWSYGCFGFGYIIPATFLPAMAK
                     DIISDPAIFGWAWPIFGAAALLSALAAGWLSTRLSYRTLWIAAHFVMAFGVAVPAVWP
                     GIGGIVLSALCVGGTFMVATMAGMQEARRVAPDNASSLMAAMTAAFAIGQILGPVLVS
                     IVAGDPHGMNLTLIAASVVLIIGALVLLPTKFMALHL"
     gene            3766..5454
                     /locus_tag="VE030_00090"
     CDS             3766..5454
                     /locus_tag="VE030_00090"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZF21842.1"
                     /translation="MHDLVIRGGIVIDGTGAPRRTADVAIDGESITGIGTGIGRGRRE
                     INARGMLVTPGFVDIHTHYDSQATWDPYLTPSSWHGVTTVIFGNCGVGFAPVRPGTAP
                     YLINLMEGVEDIPGTVLAEGVSFDWESFPEYLDVLAAAPKVMDVGAQMPHGVLRFYVM
                     GERGADHAEGPTDAEIARMGVLLEEALHAGALGLTTSRTIKHRARDGRLTPGLSASAP
                     ELFGLAAAMKRAGRGVIEVNSDFGQGEFETLRAVAAVANRPLSVLLLQVNNAPDLWRE
                     TRAQIHEARKSGLSVNGQVGCRPIGVMMGLETTAHPFSNHPAWTAMDGLSPALRFERL
                     KRDLQLQRVLVEELPDDEHTRNIGQAFARTYVLDDEYDYEPYTSNSLAARAQAQNRSA
                     WALALEALMANDGKGLLTHTFENYTEGSLEVIREMLVDEATIMGLGDGGAHVCTICDS
                     SSPTFLLSHWARDRSRGEKLPLEFLVRKQTRDTAAAYGLHDRGVLAPGYRADLNVIDF
                     ERLRLCKPEVVYDLPAGGRRLVQRAHGYSHTFVAGTETLCDDEHTGALPGRLLR"
     gene            5527..>5846
                     /locus_tag="VE030_00095"
     CDS             5527..>5846
                     /locus_tag="VE030_00095"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HZF21843.1"
                     /translation="MAEQGNWAFPEAAQPNPAEIRFDLEAALDAVVMLRAEIPEDAFT
                     ASILGTERIGNGVVIRKDGLVLTIGYLITEARAVWLTTNSGAAVAGYPLAYDQVTGFG
                     LVQPL"
BASE COUNT         1202 a         1883 c         1744 g         1017 t
ORIGIN      
        1 cttgatgaac atcatcggcg gcaagtcgtc gagataccag cttccgggaa tctcgatcag
       61 gtccgtctcg acgccgcgca ccaggggctt catccattcc tgcggcttct tcgagtagtc
      121 gatgagcgtc cagctgtcgc ctacccgcac atagtagggc tcgaaatcct tgtgcatcag
      181 gctatggtcg tacttgattc cccttttcag cagcaattcg ttggtgaccg tgctgaactc
      241 ccaccacggc gcgacatagc cggttgggcg ccggccggaa accttggtca ccaggtcgat
      301 acacttgtcg aggaccgcct cttcctgctc gcgggtcatc gagatcgggt tttcgtgggt
      361 atagccgtga atgccgattt cgtgccctgc ctccaccacc atggccgtct gttccgggaa
      421 ggtctcgatg gagtgcccgg gaatgaacca ggtggtcttg acgcccaggc gcttgaacag
      481 gttgaccaaa cgcggtgtgc cgacttcgcc ggcgaacagt ccgcgggaga tgtcgtccgg
      541 agaatcttcg cccccatagg aacccagcca gcccgcaacc gcatccacgt ctacaccgaa
      601 cgcacatagg atatctttgg ccattgtttt ctccttagtt attaagggct ctggcaatta
      661 gactggcacc atggcccgcg ccgaacccga atccaccagc ttcaacgatg tcggatggct
      721 gtccggcgcg cacatctata aaatatagac ttttacttgt caacagcatg cccgaaactt
      781 tttcaaaaaa aatagcggcc gaatcggtca agcgcacgac tctgagcgaa gccgctcatc
      841 aggcgattcg cagaaacatc attacgggag tcctggcgcc gggttccaag ctggctgttg
      901 ccgctctcgc tcaagcgctc aagctatccg ccaccccgat caacgaagca ctggccgggc
      961 tcgagcggga aggtctggtg acctattcgc cgcaccgcgg ctatttcgtg cgcacggtta
     1021 ccccggaaga tatcgaagag acctactcgg tcagagaggc tttcgaattg ctcgccgttc
     1081 gcaatgccgc gaaaaacgcc gacaaaaccg tcatagggca actcagagaa atcctccaga
     1141 aatcccggca gtctctgcgc gaaaccgaca catcccgatt cagcgacctc gacctggagt
     1201 ttcaccgcgt catctggaac agcctgaata attcactggc aaccagagtc ggcgaactca
     1261 ttggcgtcca gatccgcctg ctggtagcca ccactgctcg cgcacccggc cgcttccgtg
     1321 gtgcattcga ggagcacaac gacatctacc aggccatcag gaaccgcgat ccgacacggg
     1381 ctgaaagctc gatgaggacg catcttcaca atgccaagac cgccctggaa cacgccgttt
     1441 tcgaagaagc cggcaccggt tccaacgtct ccgctatcgg cgcttcaaaa acgaagccag
     1501 gggccaagcg tggcctcgcg cgcgaagttt cagcacaatc cgtgccaaag caaagaactt
     1561 aatttgcgtt tgtatttgaa cgcttcgttg gttggaatcc gcggaccatt gcccaggcat
     1621 ggtgcggaga ggtcgatgac ctgcacctcc attgtgcccg cttgctacgc cggtcgctcc
     1681 gttctttctt gtttcccgac tggcaccttg aagacgtcaa ggaattcaaa gccaggggcc
     1741 tgcttcatca ggcgctcgaa agtctcgagc agcgtcgccc gcggcagcca cgggtaaggc
     1801 aactccgatt ggagagcatc cagcgtcttg ccatcgcgcc gctcgatgat gtccattctc
     1861 tcgatgtgta tgtgcatcgc gcatacccgc atcctggttt catcggattc gaactgccac
     1921 ctgtaatagc ccttcccgac ccgaagcgct gaattcgaca cctccctcat gatcaccagc
     1981 cacggcatga agtctatccg caggctgcga tcgactatcg aatcggacac ataataggtc
     2041 ttgcaacggt cgaatttttt tcggaagtcc cgcaccaggg tttgcgcaat cgcatccgct
     2101 cccagtacct tgctggggaa cgagatcgtg tccgtcgcaa tggagtaagt cagaaccgcg
     2161 tttggcgcat agcattcgag aatcagggat gggcgatcaa ggtctttcgc ctccaggtac
     2221 gcctcgagaa gatccttcgg tttattcatc ctgtttacca gaaaacggca agagccggtc
     2281 ggggccacga taagcgtttt tcgacaccga cgtagaaggc cgatggcagc cgacctgcat
     2341 gggtcacaag tgaagagcca tgaacttcgt cggcaacaaa accagcgcgc caataatcag
     2401 cacgaccgac gccgcgatca gggtcagatt catgccgtgc ggatcaccgg cgacgatgct
     2461 caccagcacc ggaccgagaa tctgcccgat tgcgaatgcc gcagtcatcg ccgccatcag
     2521 gctcgaggcg ttgtccggcg cgacgcggcg cgcctcctgc atcccggcca tggtcgcgac
     2581 catgaaggtg ccgccgacgc aaagcgcgga cagcacgatg ccgccgatgc cgggccagac
     2641 ggctggtacg gcgaccccga acgccatgac gaaatgcgcg gcgatccaga gagtgcggta
     2701 gctcaggcgc gtggagagcc agcccgccgc cagtgcggag aggagcgccg ccgcgccgaa
     2761 aatcggccag gcccagccaa agatcgccgg atcggagatt atgtcttttg ccatcgccgg
     2821 caggaatgtc gccgggatga tgtagccgaa cccgaagcag ccgtacgacc agatcagtcg
     2881 catgtgcccg cccgagtgca gcggtgcaac agccgcggta cccgctgcca ccggtgcggc
     2941 tgcgccggta tatcgctgcc aaatcatcac ggtgaacagc aacgcgactg agcccattgc
     3001 gatccacgcc cggtcggcag accacgcgag ttgcagaaac accaggcaca gtccgcccgc
     3061 cagtgccgtg cccagcccca ccccgccgaa aaccactccc ccgaagcggt ggctgcccat
     3121 cgccgcgagc cgtggcagca accacgccga gccgaacacg agcgcccacg cgctggcgat
     3181 gcccgccagc gcccgtagcg ttagccatgc aagcggattg tcggtcacgc ccatacccgc
     3241 cgtcagcaat gcgatggcta ccagcgaacc gcggacgatg gcggccgatc gcgcgcggat
     3301 ccagacggcg gacagcgcgc cgatgaaata cccgacatag ttggccgacg ccagccatcc
     3361 cgccgcggcg agcgacaagc ccgcatcctt ctgcatcatg ggcaggatcg gagtgaaggc
     3421 gaaacgaccg atgccgatgg cgacggccag agtcagcatg ccggcaattg agaactgtag
     3481 tcctgatggt tgacgcaccg tgttcccgaa ggcagcagat gtgggaggga aaggttgacc
     3541 ttataaccgg ccgtccgtgg acaaacaaga cacagtcacg aaaaatttca gattgaactt
     3601 ggtggcgccc atatcaggta cgacgaccga aaagaatcgg gggccgcgtg ctgactgccg
     3661 caaatgaacc ggctggccgg cggccagcga gaggaacgtt caaccgtcac cgtctttgat
     3721 atggcgcggc aaccgcccta cacttgcaac tgaggagaat ccggcatgca tgatttggtc
     3781 atccgcggcg gcatcgttat cgacggcacc ggcgcgccgc gccgcaccgc ggacgtcgcg
     3841 atcgacggtg aatcgatcac cgggatcggc acgggaatcg gccgcggacg ccgcgaaatc
     3901 aacgccaggg gaatgctggt caccccgggc ttcgtcgaca tccacaccca ttacgacagc
     3961 caggctacat gggatccata tctcacgcct tcatcctggc acggtgtgac cacggtcatc
     4021 ttcggtaact gcggcgtagg cttcgccccg gtgcgcccgg gaaccgcgcc gtatctgatc
     4081 aacctcatgg agggcgtgga agacatcccc ggcacggtac tcgccgaggg ggtgagtttc
     4141 gactgggagt ccttcccgga atacctcgac gttctggccg cggcacccaa ggtaatggac
     4201 gtcggcgcgc agatgccgca cggggtgctg cgcttctacg tgatgggcga gcgcggggcc
     4261 gaccatgccg aagggccgac cgacgcggaa atcgcccgca tgggcgtgtt gctcgaggaa
     4321 gcgctgcacg ccggggcact cgggctcacc acttcgcgca ccatcaagca ccgcgcacgg
     4381 gacggccggc tcacaccggg actctctgcc agcgcgccgg aactgttcgg cctggcggcg
     4441 gcgatgaagc gtgccggccg cggcgtcatc gaagtcaatt ccgatttcgg tcagggcgag
     4501 ttcgagacct tgcgcgcggt agcggcggtc gccaaccggc cgctctcggt gttgctgctg
     4561 caggtcaaca acgcgccgga tctgtggcgc gaaacccgcg cacagatcca tgaagcgcgc
     4621 aaatccggcc tctcggtgaa cggccaggtc gggtgccggc cgatcggcgt gatgatggga
     4681 ctggaaacga cggcacaccc gttttccaac catccggcct ggaccgcaat ggacggcctt
     4741 tcgcccgccc tgcgcttcga gcggctcaaa cgcgatctgc agttgcagcg cgtgctggtg
     4801 gaggagcttc ccgacgacga gcacacccgc aacatcgggc aggcgttcgc tcgcacctac
     4861 gtcctggatg acgagtacga ctatgaaccg tacacttcga acagcctcgc tgccagggca
     4921 caggcacaga atcgcagcgc atgggcgctg gcgctcgagg cgctgatggc gaatgacggc
     4981 aagggcctgc tgacgcacac tttcgagaac tacaccgagg gcagcctcga agtgattcgc
     5041 gaaatgctgg tggacgaggc caccatcatg gggcttggcg acggcggcgc ccatgtgtgt
     5101 accatctgcg attcgagctc gcccactttc cttctcagcc actgggcgcg cgaccgcagc
     5161 cgcggcgaga agttgccgct ggaattcctg gtgcgcaagc agacccgcga cacagcggcg
     5221 gcctatggcc tgcatgatcg cggcgtcctc gcgcccggat atcgcgccga cctcaacgtc
     5281 atcgacttcg aacgcttgcg gttgtgcaag cccgaagtgg tctacgacct gcctgctggc
     5341 ggacggcgcc tcgtgcagcg ggcgcacggc tacagccaca cgttcgtagc gggcaccgaa
     5401 acactgtgcg acgacgagca taccggcgcg ctcccgggtc ggctgctacg gtagcgcgcc
     5461 aatcgcgtaa cattaacctc aaaccgccgg catcagcagg cgcgtttcgc gtcccttcga
     5521 tcagacatgg cagaacaggg caactgggcc tttcccgagg ccgcgcaacc gaacccggca
     5581 gagatccgct tcgacctcga ggcggcgctg gacgccgtgg tcatgctgcg tgcagaaatt
     5641 ccagaggacg cgtttaccgc ctcgattctc ggcaccgagc gcatcggcaa cggcgtggtg
     5701 atccgcaagg acgggctggt gctcaccatc ggctatctca tcaccgaggc ccgcgcggta
     5761 tggctgacga ccaacagcgg cgcggcggtc gcgggatatc cgctggccta tgaccaggta
     5821 accggtttcg gtttggtgca gccgct
//
LOCUS       DASQOP010000004        17991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_806830, whole genome shotgun sequence.
ACCESSION   DASQOP010000004 DASQOP010000000
VERSION     DASQOP010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 17991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17991
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806830"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..1506
                     /locus_tag="VE030_00100"
     CDS             10..1506
                     /locus_tag="VE030_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HZF21844.1"
                     /translation="MVVIGSGVGGGAVAFTLADTGARILILERGERLPREAENWSPEA
                     VFVEQRYRTQETWYDGNGKPFRPGQFYFVGGHTKVYGTAMFRFRERDFEGIEHADGKA
                     MPWPVRYADLEPWYGEAEKLFGVHGRAGDDPTEPPRSTPYPHAPIPHEPIIGKAFERM
                     RALGLHPFHMPSAADLHPGGTCVRCGTCDAFPCRVGAKGDAETRLINPALKHSNVALQ
                     TGAEVTRLVADQRGKRIVAAEYRRGGNLLSVSARLFVLSAGAINSAALLLRSADAHNP
                     QGLANSSGVVGCYYMNHNCTGIMALTPLHVNDTRFPKTLALNDFYFASGESDKPLGNL
                     QLLGKIQEPMLRAALPRTPKWLRTWLARHSVDWYVMSEDLAQRDSEVRVRNDGAIELR
                     WKRSNLRAHHRFVEVAKKLLKDIGFPIVLSKPFGTDTPSHQCGTARFGTDPAASALDP
                     YCRAWDHDNLYVVDASFFPSSAALNPALTIAAQALRVGSHLKACLHEF"
     gene            1508..1651
                     /locus_tag="VE030_00105"
     CDS             1508..1651
                     /locus_tag="VE030_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF21845.1"
                     /translation="MAHSRPVDLVTGAPRGIGAAVALASGRFRFATGAAIIVDGGLWL
                     PRP"
     gene            1708..3318
                     /locus_tag="VE030_00110"
     CDS             1708..3318
                     /locus_tag="VE030_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HZF21846.1"
                     /translation="MPDDLFDYIITGGGSAGCVLAHRLSADPSAKVLLLEAGGSDRHP
                     FFHMPAGFAKMTKGIGSWGWLTVPQKHLKNRILRFTQAKVIGGGSSINAQIYTRGVPA
                     DYDEWARDGGAAGWSYREVLPYFKRAENNQRYANEYHGYGGPLGVSNPISPLPICEAF
                     FQAGQEMGIPFNPDFNGELQEGLGYYQLTQSNARRSSTSVAYLNPIRHRRNLTVRLRA
                     QALRVVIERGRATGIEVAVGASRTPQLLRARREVIVTSGAIGSPKLLMQSGIGPADHL
                     RSVGLNVVHDLPGVGGNLQDHLDLFVIAECTGDHTYDKYNRPLRAAWAGLQYLLLRSG
                     PVASSLFETGGFWYADKGSRSPDIQFHLGLGSGIEAGVTKMTNPGVTLNSAYLRPRSR
                     GTVRLASADPAAAPLIDPDYWAETRDRDLAIEGLRLAREILRQRALKRFVMAERLPGD
                     ALKTDADLFEYACVHAKTDHHPVGTCRIGPENHRTSVVTPDLRVIGLEGLRVADASVM
                     PSVPSCNTNAPTIMVAEKAADHILGILA"
     gene            3328..4497
                     /locus_tag="VE030_00115"
     CDS             3328..4497
                     /locus_tag="VE030_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HZF21847.1"
                     /translation="MQLTLPKEDGSLRPYTQVNDVSAFAAVQPARWNRTAYAAAHVVV
                     DPLKPCDPWDGSPVVDWDATLRFREYLWRLGFKIAEAMDTAQRGMGLDWPTALELIRR
                     SIAHARTIPGADLACGVGTDQLAADARPTIADVKHAYEEQIEAVEAAGGRIILMASRA
                     LAAAARSVDDYRRVYGDLLGQCKDKVVLHWLGEMFDPKLGGYWGSADIPRAMEAVLDI
                     VNANAAKIEGIKISLLDAKWEVEMRRKLPPGVLMFTGDDFNYDTLIAGDGTQHSHALL
                     GIFDPIAPIASAALARLAVGNAAAYKELMTPTIPLSREIFRAPTRHYKAGVIFIAWLN
                     GHQSHFVAAGGLQSARSVLHYARVFELADRCGALIDPELAGTRMRAFLQVHAGIS"
     gene            4494..5432
                     /locus_tag="VE030_00120"
     CDS             4494..5432
                     /locus_tag="VE030_00120"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HZF21848.1"
                     /translation="MTASQPAPRFVSAGEALTDMIRQEGGYWLARAGGAPWNVARVMA
                     GFGIASAFAGSVSRDNFGDEIAALSRAAGLDMRFLQQHDKPSLLAIVHETSPPRYYFI
                     GADSADLAFDPSVLPPDWTRSVEWVHCGGISLAREPLCSRLIVLLEDAKAAGARVSFD
                     PNFRNLMTPAYDTTLARVARLADVIKVSDEDLRGLFRTRDRRSALAQLNALNPRAVIL
                     LTLGAEGAELHTQGKRLHQESPTVEIVDTVGAGDASIGGLLYSLMHRPDAGWPAHLRR
                     AVAAGTAACLLPGAAPPSLAAVEKVLATMDQPRSVD"
     gene            5435..6634
                     /locus_tag="VE030_00125"
     CDS             5435..6634
                     /locus_tag="VE030_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HZF21849.1"
                     /translation="MVMPDFRDPRFLLDHIRHTMAFYHPRAIDPHGGFFHFFRDDGSV
                     YDAHTRHLVSSTRFVFNYAMAYRRFGDAAYLRAVEHGTAYLRERHRNASTGGYAWLLD
                     SDRVADATNHCYGLAFVLLAYAHASMAGVAPAREWIGETFDLMEQRFWSAQFGLYADE
                     ASPDWSVLSPYRGQNANMHSCEAMLAAFEATGETRYLERAATIARNVTVRQAALAHNM
                     VWEHYRTDWSVDWDYNRNDRTNIFRPWGYQPGHLTEWAKLLLILERHKARLSTPPDWL
                     VLRATELFDAALERAWDAEYGGIYYGFAPDGGICDADKYFWVQAESLAAAALLAARTG
                     DEKYWQWYEKIWAYSWEYFVDHKYGAWYRILSRGNEKYSAEKSPAGKTDYHTMGACYE
                     VLNVIGQ"
     gene            complement(6739..7407)
                     /locus_tag="VE030_00130"
     CDS             complement(6739..7407)
                     /locus_tag="VE030_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZF21850.1"
                     /translation="MKAVSRLAALGVLLFIVACGPETNHNQAVYMLIDTSGTYARETG
                     KAQQVVNYLLGSLNPGDALAVGRVKSRSFSEKDIVAKVSLGKDPLAANAQKRAFSEQI
                     AAFTKTTKDAQGSRYTDITGGMLQAAEFLNESQAGRKTIVIFSDMQEELDYKTVRDFP
                     IRLDGIRVVALNVTKLETDNVDPRRYIDRLEWWERRARTAGASDWKVVNDMGHLDRIF
                     KKKG"
     gene            complement(7404..8879)
                     /locus_tag="VE030_00135"
     CDS             complement(7404..8879)
                     /locus_tag="VE030_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF21851.1"
                     /translation="MSLLESLFIWPNRPLPSLLVLLVLLMPFLYAAREPMHALLRSMC
                     RSIANPLRLGARWLADAGLRLRNRNREVLFAHGSHEVKLTIEREFEHVTMLVQRDLEG
                     YPVLQRKLMDEITRIEEDYKKSGEVPPPPPEWVKAIEAIAKIKPSGDGVVERILSDIR
                     DSLDEIYQKLVAEYRGAYQERHHILKGFLPFWRSVSQTLTQVDRNITSLQNSAAKIDA
                     NVLKLENIAARGNDAANVLATSATTQFFIAGLVMLIAFGGAYVNFKLIALPMSAMVGG
                     GDYITESLQASEVAALVIILFETLMGLFLMETLRFTSLFPLGNITEKMRRRLMWASLT
                     ILLVLAGVEVALAVMRDMIIAADVALKQQLGAAAGAAKVESGWVTRIPTFGQMILGFT
                     LPFALAFVAIPLEYFVNSGRTVVGAGLEMALRGLAFILRFAANLIRGAGKLAIMLYDV
                     FIFVPLAIERGIVSYREGAARRNKLRPVPPLKRASGSEEVL"
     gene            9210..9410
                     /locus_tag="VE030_00140"
     CDS             9210..9410
                     /locus_tag="VE030_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF21852.1"
                     /translation="MGKEKIVQRKPVSRARMVRRVIGVGLWAGGITSGIIGGDYSLPI
                     ALALFVIGFFLFVSTPGAKTRH"
     gene            complement(9608..9913)
                     /locus_tag="VE030_00145"
     CDS             complement(9608..9913)
                     /locus_tag="VE030_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF21853.1"
                     /translation="MTTELANTELTPADVPAVGESWTRIEPFALTFDGFRYWGSVEKC
                     AEVAGQTPGTLTDLRTCLFFEARRWNNLGREPDAASMKRICALISVIQQKIKAGDRR"
     gene            10079..10348
                     /locus_tag="VE030_00150"
     CDS             10079..10348
                     /locus_tag="VE030_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF21854.1"
                     /translation="MAAPAPSPITDPLRDLAAKIYIELICRNVVVTENAAQIKSNPES
                     LAKISFKLAEAFQRVGVEVKAPSLPKNQEFDMKATDISDWNAAKP"
     gene            complement(10486..10689)
                     /locus_tag="VE030_00155"
     CDS             complement(10486..10689)
                     /locus_tag="VE030_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HZF21855.1"
                     /translation="MATGTVKWFNDAKGFGFITPDGGGKDLFAHFSAIQGNGFKSLQE
                     NQKVTFDVTTGPKGDQASNIRPA"
     gene            complement(10967..11404)
                     /locus_tag="VE030_00160"
     CDS             complement(10967..11404)
                     /locus_tag="VE030_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HZF21856.1"
                     /translation="MKNRIKITAGPFVFVARMEIEKASATCAKFAKLLPFRNKVIHSR
                     WSGEAVWIPLGAMDTGLEFENHTSHGSRGDLLFYPGGFSETEILFVYGSSRFASKMGQ
                     LAGNHFLTVEDGRENLEAFGKLVLWHGAQDILFERMPEAAGGT"
     gene            complement(11514..12923)
                     /gene="hydA"
                     /locus_tag="VE030_00165"
     CDS             complement(11514..12923)
                     /gene="hydA"
                     /locus_tag="VE030_00165"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HZF21857.1"
                     /translation="MAATLIKNGRIVTAVDDYTADIVIVDGRIQTIGRNIAVGADVKV
                     HDANGLLVLPGGVDVHTHLDWEFGPTHTVDTFETGTKAAAFGGTTTLIDFCNQTTGKS
                     PLVGLEDWHKRRASACVDVGAHMIMLDVNEQSLADMKTLVRREGVTSFKLFMAYPGVL
                     MVDDGALFKAMRVAGANGAMTCIHAENGPVIQVLVQEAVAQGLRAPKYHATTRPSILE
                     GEATHRAICLAELAGTPLYIVHLSAGEALSAVTEARDRGIPVHAETCPHYLFLTAQDY
                     DRPGFEGAKYVMTPPLRDHNHQQHLWRGLKTDDLQVVSTDHCPFCFNEKPYGMKFSKQ
                     QGADSFSKIPNGAPGVETRLPLIFDGAVKKQGMSINRFVGITSTAPAKLFGLFPRKGA
                     IAVGSDGDIVLFDPDEQWTIRAAEQHSRVDYTLFEGRPVTGRVKKVFLRGQCIVDGTG
                     WLGRAGMGEYLTRGESGKI"
     gene            complement(12976..13962)
                     /locus_tag="VE030_00170"
     CDS             complement(12976..13962)
                     /locus_tag="VE030_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HZF21858.1"
                     /translation="MKPKIVLLATGGTIASRYDPKLGSTVASQRADELLRMLPQVADF
                     AEIEIHDIATIPSYEMNVQFAFDLAKRIKEMLGGPEVAGVVVTHGTDTMEETCYLADL
                     LLDSDKPAVYTGAQRAHDDPQSDGPINLLNALRVAAAPSARGLGAMICFNGAIHAARD
                     VTKVHASAVETFQSYEHGALGQVDGAEVIIHRRPVLRHSFKVDQLEERVELFRLTLGV
                     DLRGLEAALERGVAGLVIEGFGRGNGPSRLAELVRLATRKNVPVLITSRCPVGRVQPV
                     YGGGGGGRDLADAGGIFAGDLKGPKARLLLMVLLSSAKTRTRIAETVAALAP"
     gene            complement(13976..15214)
                     /locus_tag="VE030_00175"
     CDS             complement(13976..15214)
                     /locus_tag="VE030_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HZF21859.1"
                     /translation="MAAISIDSQRLWKSLMDLARIGATEKGGVRRLALSRLDGEARDL
                     FICWCKDAGCSVAIDGIGNIFARRPGKDNGLPPIITGSHIDTQPSGGKFDGNYGVMAG
                     LEVIRTLNDHKISTEAPVEVAVWTNEEGSRFTPVMMGSGVFVGAFALDSTLQRADLEG
                     VTVGEALEQIGYAGKEKIGGRPVGAYFEAHIEQGPILEDTRKVIGVVVGALGLRWYDV
                     VVTGQDAHAGPTPMRLRKDALVAASKMVAEINALAFRYQPDGRGTVGFMQVKPNSRNV
                     IPGEVRFSVDLRHAQDAALDRMEGEMRAICERLAQTGNVNSSVERVANYPACNFDSGC
                     VSAVRNAAQTLGLSYMDIVSGAGHDAIYMARVTPTSMIFVPCEGGISHNEIENAKAED
                     LAAGCNVLLNVMLEKARAAN"
     gene            complement(15281..16612)
                     /gene="preA"
                     /locus_tag="VE030_00180"
     CDS             complement(15281..16612)
                     /gene="preA"
                     /locus_tag="VE030_00180"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HZF21860.1"
                     /translation="MADLRCNFVGIHSPNPFWLASAPPTDKAYNVNRAFEAGWGGVVW
                     KTLGEDPPIVNVSGPRYGALLGTARNLVGFNNIELITDRMLDDNLREIRQVKRDWPDR
                     ALAVSLMVPCEEQAWKRILPMVEDTGADGIELNFGCPHGMSERGMGSAVGQVPEYVEM
                     VTRWCKEHTHLPVIVKLTPNVTNILQPARAALRGGADAVSLVNTISSIMGVDLDLMSP
                     SPSVDGKGAHGGYCGPAIKPIALRMVGEIARDRESAGMSISGIGGISTWRDAAEFMAL
                     GAHNVQVCTAAMVYGFKVVEDMIDGLNNWMDEKGHRTLSAFVGKAVPNFVDWEHLNLN
                     YTVKAHIDQDLCIKCGRCYIACEDTSHQSIRNEKDGRRHFEVIDEECVGCNLCVNVCP
                     VENCITLKELAIGSIDPRTGRAVQGYGSWSTHPNNPTPQAEKGKPVAGLLK"
     gene            complement(16605..17984)
                     /locus_tag="VE030_00185"
     CDS             complement(16605..17984)
                     /locus_tag="VE030_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZF21861.1"
                     /translation="MSDRDSKSDIRGGRLAAEEYARNFADAHPPLAPNQALIESDRCY
                     FCHDAPCIRACPTQIDIPSFIRKIATSNVKGAAHDILKQNIMGAVCARVCPTEVLCEG
                     ACVRNDQEHKPVAIGALQRHATDWLFDRRIQLFERGPETGRRIAVVGGGPAGLSCAHR
                     LSMLGHSVTVFEARDKLGGLNEYGVAAYKVTGLIAQREVEYLLEIGGIENRLRQRLGR
                     DIVLSRLRQDYDAVFLGIGLGGVNALSVDEPPAGVFDAIAYIEKLRQSRDLSQMPVGH
                     GVVVIGGGMTAIDIASQVKRLGAEDVTIVYRRGPQSMGASHKEQAWAQTSGVRIKHWA
                     RPLRLLARDGVLTAVEFEQTRLDSQGRLAGGGDKFIVAADMVFKAIGQMLIKDALDGN
                     ADVLEMKSGRIAVDAERRTSIPGVWAGGDCVAGGQDLTVVAVEDGKRAALSIDRHLRA
                     VSKTEARHG"
BASE COUNT         3308 a         5790 c         5460 g         3433 t
ORIGIN      
        1 tcctacgacg tggtcgtcat cggctccggc gtcggtggcg gagcggtcgc gtttacgctt
       61 gccgacaccg gcgcgcgcat cctgatcctg gagcgcggag aacgcctgcc cagggaagcc
      121 gagaactgga gcccggaggc agtgttcgtc gaacagcgct accgcacgca agagacctgg
      181 tacgacggca acggcaaacc attccgtccg ggccagttct acttcgtcgg tggccatacc
      241 aaggtttacg gcacggcaat gttccgcttc cgcgaacgcg atttcgaggg catcgagcat
      301 gccgacggca aggccatgcc gtggcctgtc cgctacgccg atttggaacc ctggtacggt
      361 gaggccgaaa aactgttcgg agtgcacggc agggcgggcg acgatccgac cgaaccgcca
      421 cgatcgacgc cctacccgca tgcgccgatt cctcacgagc cgatcatcgg caaggcattc
      481 gaacgcatgc gcgcgctcgg cctgcacccc tttcacatgc cctcggcggc cgacctccat
      541 cccggcggca cgtgcgttcg ctgcggcact tgcgatgctt tcccgtgtcg agtcggcgcc
      601 aagggcgacg ccgagaccag gctgatcaac ccggcattga agcattcgaa cgttgcattg
      661 cagaccggag ccgaagttac ccggctcgtc gccgaccaaa ggggtaagcg tatcgttgca
      721 gcggagtacc ggcgcggcgg caacttactt tccgtcagcg cacgcctgtt cgtgctgtcg
      781 gccggcgcaa tcaacagcgc ggcattgctg ctgcgttcgg ccgatgcgca caacccgcag
      841 ggcctggcca actcctcggg cgtggtcggc tgctattaca tgaaccacaa ctgcaccggg
      901 atcatggcgc tgacaccgtt gcatgtgaac gacacgcgct ttccgaagac gcttgcgctc
      961 aacgatttct acttcgccag cggcgagagc gacaaaccgt tgggcaatct gcagctgctg
     1021 ggcaagatcc aggagccgat gctgcgcgcc gcattgccgc gcacgccgaa atggctgcgc
     1081 acctggctcg cgcgccacag cgtggattgg tatgtgatgt ccgaggacct ggcgcaacgc
     1141 gacagcgaag tcagggtgcg caacgacggc gcgatcgagt tgcgctggaa gcgcagcaac
     1201 ctgcgcgcgc accaccgctt cgtcgaagtg gcgaagaagc tgctgaagga catcggcttc
     1261 ccgatcgtgt tgtcgaagcc gttcggtacc gacacgccct cgcaccagtg cggcaccgcg
     1321 cgcttcggca ccgacccggc ggcttccgcg ctcgatccgt attgccgcgc ctgggatcac
     1381 gacaacctgt acgtggtgga cgcgagcttc tttccttctt ctgccgcgct caacccggcg
     1441 ctgacgattg cggcccaggc gctacgcgtg ggcagccact tgaaggcatg ccttcacgag
     1501 ttctgacatg gcccattccc gtcccgtgga cctggtcacc ggcgcgcctc gcggcatcgg
     1561 cgccgccgtg gcactggcga gcggacgttt ccggttcgcc accggcgcgg caatcattgt
     1621 ggacggcggg ttgtggttgc caagaccttg aggccgattg cgttcaatcc tttcgagtcg
     1681 gaaaatttgc acaggcgctg acccaaaatg ccggacgacc tcttcgacta catcatcacc
     1741 ggcggcggct cggcaggctg cgtgctggca caccgcctga gtgccgaccc gtcggctaaa
     1801 gtgctactgc tggaagccgg cggttccgac cgtcatccgt tcttccacat gccggcgggc
     1861 tttgccaaga tgaccaaagg catcggctcc tggggctggc tcacggtgcc acagaagcat
     1921 ctgaaaaacc gcatccttcg cttcactcag gccaaggtca tcggcggcgg ctcgtcaatt
     1981 aacgcgcaga tctacacccg cggcgtaccg gctgactacg acgaatgggc tcgcgacggg
     2041 ggtgcagccg gctggtcgta ccgcgaagtg cttccatact tcaaacgggc cgagaacaac
     2101 cagcgttacg caaacgagta ccacggctat ggcgggccgt tgggcgtgtc gaacccgatc
     2161 agcccgctgc cgatctgcga ggccttcttc caggccgggc aggaaatggg catccccttt
     2221 aacccggact tcaacggtga gctccaggag gggctgggct actatcagct tacccagtcg
     2281 aacgcgcggc gctcgtcaac ctcggtggct tacttgaatc cgatccgcca ccgccgcaat
     2341 ctgacggtac ggctgcgggc acaggcgttg cgcgtggtga tcgaaagggg acgcgcgacc
     2401 ggcatcgagg tcgcggtcgg agcttcgcgc acaccgcagc tcctgcgtgc aaggcgcgaa
     2461 gtcatcgtga cctcgggcgc gataggttcg cccaaactct tgatgcaatc gggcatcggg
     2521 ccggccgacc acctgcgctc ggtcggcctg aatgtggtgc acgacctgcc cggcgtgggc
     2581 ggcaatctgc aggaccatct cgatctgttc gttattgccg agtgcaccgg cgatcacacc
     2641 tacgacaagt acaaccggcc gctgcgtgcg gcatgggccg gccttcagta cctgctgttg
     2701 aggtcgggac cggtggcttc cagcctgttc gaaaccggcg gcttctggta tgcagataag
     2761 ggctcgcgct cgccggacat tcagtttcat ctcggcctcg gctccggcat cgaagccggc
     2821 gtgaccaaga tgacgaatcc aggagtcacg ctcaatagcg cctaccttcg cccgcgctcc
     2881 cgcggcaccg tgcggctggc cagtgccgac ccggccgcgg caccgctgat cgatcccgac
     2941 tactgggccg agacgcgcga ccgcgatctc gcgatcgagg gcctgcgcct cgcgcgcgag
     3001 atcctgcggc aacgcgcgct aaaacgcttt gtgatggccg agcgactgcc cggggatgca
     3061 ttgaagaccg acgcggacct tttcgagtat gcctgcgtgc acgccaaaac cgaccaccat
     3121 ccggtgggca cctgccgcat cgggccggag aaccatcgga ccagtgtggt cacgccggac
     3181 ctgcgagtca tcggcctcga gggcctgcgg gtggccgatg cgtcggtgat gccgtccgtg
     3241 ccttcatgca ataccaatgc gccgaccatt atggtcgcgg aaaaagccgc ggaccacatc
     3301 ctcggcatac tcgcctaggg gcccctcgtg caattgacgc tgccgaaaga ggacgggtcg
     3361 ctgagaccat acacccaggt gaacgatgtt tccgccttcg ccgcggttca gccggcacgc
     3421 tggaaccgca ccgcctacgc ggctgcgcac gtcgtcgtgg acccgctcaa accctgcgat
     3481 ccgtgggacg gttcacccgt ggtggattgg gacgcgacgc tgcgctttcg cgagtacttg
     3541 tggcgattgg ggttcaagat cgccgaagcg atggacacgg cgcagcgcgg tatgggcctg
     3601 gactggccga ccgcactcga attgatccgc cgcagtatcg cccatgcccg gactatcccc
     3661 ggcgccgatc tcgcctgtgg tgtcggcacc gatcagctcg cagcggatgc ccgccccacg
     3721 atcgccgacg tgaagcacgc ctacgaagag cagatcgaag cggtggaggc agccggtggg
     3781 cgcattatcc tgatggcgag ccgtgcgctc gccgccgcgg cgaggagtgt cgacgactat
     3841 cggcgcgtgt acggcgatct gcttggccaa tgcaaggaca aggtggtgct gcactggctg
     3901 ggagagatgt tcgatccgaa gctgggtggt tattggggca gcgccgacat cccgcgggca
     3961 atggaagcgg tgctggacat cgtgaacgcc aatgcagcga agatcgaggg catcaagatc
     4021 tcgctgctgg acgcaaaatg ggaagtcgag atgcgccgca agttgccgcc cggcgtactg
     4081 atgttcaccg gcgacgactt caactacgac acgctgatcg ccggcgacgg cacgcagcat
     4141 tcgcacgccc tgctcggcat cttcgacccg atcgcgccga ttgcatcggc cgccctcgcc
     4201 aggctcgcgg tcggcaatgc cgcagcttac aaagagctga tgacgccgac catccccttg
     4261 tcgcgcgaaa tttttcgtgc tccaacgcga cactacaagg ccggcgtaat tttcatcgcc
     4321 tggctgaacg gccaccagtc gcacttcgtc gccgccggcg gactgcaatc ggcgcgctcg
     4381 gtactgcact atgcgagagt gttcgaactg gccgaccgct gtggcgcact catcgatcct
     4441 gagctcgcgg gtacgcgcat gcgcgctttc ctgcaggtcc acgccggcat ctcatgacag
     4501 catcgcagcc tgcaccgcgc ttcgtcagtg ccggcgaagc actgaccgat atgatccggc
     4561 aggaaggcgg ctactggctg gcgcgcgccg gcggcgcacc gtggaacgtg gcgcgtgtca
     4621 tggcgggctt cggcatcgcc agtgcgtttg ccggcagcgt cagccgcgac aacttcggcg
     4681 acgagattgc cgcgctcagc cgcgcggccg gactggacat gcgcttcctg caacagcacg
     4741 acaaaccctc gttgctggcg atcgttcatg aaacctcccc gccacggtat tacttcatcg
     4801 gcgcggacag tgccgatctc gccttcgatc cgagtgtgct tcccccggat tggacccgat
     4861 cggtcgaatg ggtgcactgc ggcggcatca gcctcgcgcg cgaaccgctg tgcagccggc
     4921 tgatcgtctt gctggaagac gcgaaggcgg cgggcgcgcg cgtgagcttc gatccgaatt
     4981 tccgcaatct gatgacgccc gcttacgaca cgactctggc ccgcgtggca cgcctggcgg
     5041 acgtgatcaa ggtttccgat gaggatctgc gcggcctgtt ccgcacccgg gatcggcgca
     5101 gcgcattggc gcagctcaac gcactcaatc cacgggcggt cattctgctg acgctcggcg
     5161 ctgagggcgc ggaactgcat acgcaaggaa agcgtttgca ccaggaaagc ccgacggttg
     5221 aaatcgtcga caccgtcggc gccggcgacg ccagcatcgg cggcttgctc tacagtctga
     5281 tgcaccgacc agatgccggg tggccggcgc atttgcgccg cgcggtggcc gcgggcacag
     5341 cggcctgcct gctgcccggc gccgcgccac cgtcccttgc cgcagtcgaa aaagtcctcg
     5401 cgaccatgga tcaaccacgg tcagtggact gacgatggtc atgcccgact tccgcgaccc
     5461 ccgcttcctg ctcgaccata ttcgccacac gatggcgttc taccatccgc gcgcgatcga
     5521 tccgcacggc ggattttttc attttttccg cgatgacggc agcgtctacg acgcgcatac
     5581 ccgtcacctg gtcagcagca cgcgtttcgt tttcaactat gcgatggcgt atcgccggtt
     5641 cggcgatgcc gcgtacctgc gggccgtgga gcatggcacg gcttacctgc gtgaacggca
     5701 tcgcaacgcg tcgacgggtg gatacgcctg gctgctcgac agcgatcgtg tcgccgacgc
     5761 gaccaatcat tgctacggcc tcgcattcgt cctgctggcg tatgcgcacg catcgatggc
     5821 cggcgtcgca cccgcgcggg aatggatagg cgaaaccttc gatctgatgg agcagcgatt
     5881 ctggtcggcg cagttcggcc tgtacgccga cgaggcaagt ccggactggt cggtgttgtc
     5941 accctaccgc ggacagaatg ccaacatgca ttcctgcgaa gccatgctgg cggcattcga
     6001 agccaccggc gagacgcgtt atctcgagcg tgccgcgacc atcgcccgca acgtcaccgt
     6061 gcgccaggcg gcgctggccc ataacatggt gtgggagcac taccgcaccg actggtcggt
     6121 ggactgggac tacaacagga acgacaggac caacatcttc cgaccctggg gctatcagcc
     6181 cggccatctc accgagtggg cgaagctgct actgattctg gaacgccaca aggcgcggct
     6241 ttcaacgccg cccgactggc tggtgctgcg cgcaacagaa ttgttcgacg ctgcattgga
     6301 gcgggcatgg gatgcggaat acgggggaat ctattacggc ttcgcaccag acggcggcat
     6361 ctgcgatgcc gacaagtatt tctgggttca ggcagaatcg ctggccgccg cagcactgct
     6421 tgctgcaagg accggcgatg agaagtattg gcagtggtac gaaaagatct gggcctacag
     6481 ctgggaatac ttcgtcgacc acaagtatgg cgcctggtac cgcattctct cccgcggcaa
     6541 cgaaaaatac agcgccgaga agagcccggc gggcaagacc gactatcaca ccatgggcgc
     6601 gtgctatgag gtgctcaacg tcatcgggca atagaacgct aatgagccga cgtcggggca
     6661 aatcgtgaag atgcagccga aggctgccaa aacaaaacgg ccgggaattc ccggccgttt
     6721 gattgcgtgt tggactgctc agcctttctt tttgaagatg cggtcgaggt gccccatgtc
     6781 gttgacgacc ttccagtccg acgcaccggc cgtacgggcg cgtctttccc accactccag
     6841 ccggtcgata tagcgacgcg ggtccacgtt gtcggtttcg agcttggtga cgttgagagc
     6901 gaccacgcga atgccgtcga gcctgatcgg aaagtcccga accgtcttgt agtcgagttc
     6961 ttcctgcatg tccgaaaaga tcacgatggt cttgcgcccg gcctgggatt cgttcaggaa
     7021 ttcggccgcc tgcaacatgc cgccagtaat gtcggtgtag cggctgcctt gtgcgtcctt
     7081 cgtggtcttg gtgaaggcgg caatctgctc actgaacgcg cgcttttgcg cgttggccgc
     7141 gagcgggtcc ttgcccaggc tgactttggc cacgatgtcc ttctcgctga aactgcggct
     7201 cttgacccgc ccgaccgcaa gcgcgtcgcc gggattgagc gagcccagaa ggtagttcac
     7261 cacctgttgc gccttgcctg tttccctggc gtaggtgccg gaggtatcga tcagcatgta
     7321 caccgcctgg ttgtgattgg tttcaggccc gcacgcaacg atgaacagca gtaccccgag
     7381 cgccgcaagt ctggacaccg ctttcatagc acctcctcgg atccggaagc acgcttgagc
     7441 ggcggcaccg ggcgcagctt gttgcggcgg gcggcaccct cgcggtaact gacgatgccg
     7501 cgctcgatcg caagcggcac gaagatgaac acgtcgtaga gcatgatcgc cagcttgccc
     7561 gcccccctga tcagattggc ggcaaagcgc aggatgaacg ccagtccgcg caacgccatc
     7621 tccaggcccg ctccgaccac ggtacgaccg gagttgacga agtattcgag cgggattgcc
     7681 acgaacgcca gcgcaaaggg cagcgtgaag cccaggatca tctggccaaa agtcgggatt
     7741 ctggtgaccc aacccgactc gaccttcgcc gcgccggcgg cggcgccgag ctgctgcttc
     7801 agtgccacgt ctgcggcgat gatcatgtcg cgcatgaccg ccagcgccac ttcaacgccc
     7861 gccagaacaa gaaggatcgt gagcgatgcc cacatcagcc ggcgccgcat cttctcggtg
     7921 atgttgccca acgggaacag gctggtgaag cgcagcgtct ccatcaggaa caggcccatc
     7981 agggtttcga acaggatgat cactagcgcc gccacttccg aagcctgcag gctctcggtg
     8041 atgtagtccc cgccgccgac catcgccgac atcggcagcg cgatcagctt gaagttgaca
     8101 taggcgccgc cgaaggcaat cagcatcacc aggccggcga tgaagaactg cgttgtggct
     8161 gaggtcgcga gtacgtttgc agcgtcattg ccgcgggccg cgatgttttc cagtttcagg
     8221 acattggcgt cgattttcgc cgcgctgttc tgcagactgg tgatgttgcg gtccacctgg
     8281 gtcagcgtct ggctgaccga gcgccagaac ggcaggaagc ccttcaatat gtggtgtcgc
     8341 tcctggtatg ctccgcggta ttccgccacg agcttctgat aaatttcgtc gagcgagtcg
     8401 cgaatgtcgg agagaatgcg ttccaccaca ccgtcgccgc tgggcttgat cttggcgatg
     8461 gcctcgattg ccttgaccca ctccggcgga ggcggcggca cttcgccgct cttcttgtag
     8521 tcctcctcga ttcgcgtgat ttcatccatc aacttgcgct gcagcaccgg gtacccctcg
     8581 aggtcacgct gcaccagcat ggtaacgtgc tcgaactcgc gctctatcgt cagcttcact
     8641 tcatggctgc catgcgcgaa tagcacttcg cggttgcggt tgcgcaggcg caaaccggca
     8701 tcggccagcc agcgcgcgcc cagccgcagc ggattggcga tcgaccggca catgcttctg
     8761 agcagcgcat gcatcggttc gcgcgccgca tacagaaatg gcatcaacag aaccagcagc
     8821 accagcaggg atggcaacgg gcgattcggc cagatgaaca gcgattccag gagtgacatg
     8881 gttccccctt gttgtctgac tttggcttcg gtaacaaaac aacacgcgaa acagagttcg
     8941 cgtgcctgaa cccattttta tctgcaaccg gcgggccaga aatgcgccca acctcgcata
     9001 tcggcacggt ttgcggaccg gtgcgcttac cctcctgtta ttgcgcgaat aaatcggtgt
     9061 cagggagccg ggaaaccgct gggtgatcga gggcaagtgt cgccgcgcag cgacttgggg
     9121 tgccgaaatt ggtaacaccc gtgcgggcag gggtaacttg ctgatcgatt gggcgaggaa
     9181 gttacctttc ggtgcagggg aggtgcgtga tgggaaaaga gaaaatcgtg cagcgcaagc
     9241 cggtttcccg ggcccggatg gtgcgccgcg tcatcggcgt cgggctgtgg gccggcggaa
     9301 tcacatccgg gatcatcggc ggcgattaca gcctgccgat cgcgctggca ctgttcgtaa
     9361 tcggcttctt cctgttcgtc tccacgcccg gggccaagac aaggcactaa cgtgctgggt
     9421 agaaatcacc ggtgactcag aactcggcca aggactggcg cgtgatgttt acagcaaaga
     9481 aacgagacct ccggaatctt gagttactga caccgtatgc ggccaagttt cgcaattgac
     9541 gttgcgggac aaacttgagg tgttacagat atcggtccac gacatccaga atagccacgg
     9601 catttagtca gcgcctatca ccggctttaa ttttttgctg aatcacgctt atcagcgcgc
     9661 atatgcgctt catcgatgcg gcgtccggtt cgcgtccgag gttattccaa cgccgcgcct
     9721 cgaagaacag gcaggttcgc aggtccgtca gcgttcccgg ggtctggcct gcgacctcgg
     9781 cgcacttttc aactgacccc cagtagcgga agccgtcgaa agtgagggca aagggttcga
     9841 ttcgcgtcca ggactcgccg accgcgggca cgtccgccgg tgtaagttcc gtgtttgcca
     9901 gttcggtcgt catgagcgga cagcagcaat taacaggccc aagtctctat tgttctctat
     9961 cttctcggaa aaagacacgc gcgccgcctg ctgtctgtcg cggcccggcg acacgagagg
    10021 agaaagcgct gcgcctattg gcgtaggatg cacggtaccc ctacttcaag gcagacccat
    10081 ggccgctccc gctccatccc cgatcactga tccattgaga gacctggctg cgaagatcta
    10141 catcgagctg atttgccgca acgtcgtcgt taccgagaat gcggcccaga tcaaatccaa
    10201 ccccgagagc ctcgccaaaa tcagcttcaa actggccgag gccttccagc gcgtgggcgt
    10261 ggaagtcaaa gcccccagcc tgccgaagaa tcaggaattc gacatgaagg cgaccgatat
    10321 ttctgattgg aacgcggcca agccgtgaga atggcgggga cgattgccgc taacgcatcg
    10381 caacgtcggc tggaaaagaa aaaagcccgg ttccccgggc ttttctcttt gagtgccgag
    10441 ttggcctggt cgaaatccgc caggcaaccg gcactgccgt caggcttacg cgggacggat
    10501 attcgaggcc tgatcacctt tcggaccggt ggtcacgtcg aacgtgacct tctggttttc
    10561 ctggagtgac ttgaagccgt tgccctgaat cgcggagaaa tgcgcaaaaa gatccttgcc
    10621 gccgccatcc ggagtaatga aaccgaaacc cttcgcgtcg ttgaaccact tgacggtacc
    10681 tgtagccata aatttacttt cctaataaaa attgaaaatg ggcggtttgc ccgagtgcgg
    10741 atacttcaag aggagaaatt aaggagatct gaggggtacc gaaaacggat acaacggacc
    10801 agcaatatca gcgcttggag aatcctgcgc cgcctttata cgcccttatg tgggcagggt
    10861 caacgcattt ctggagtcgg cgcggatacc cgggcgcttc ggcccaggtc cgtgattcgc
    10921 cggatcacgg cttatcagtc acgagtaatt tgtcgccgcg agccggtcag gtaccgccag
    10981 ccgcctccgg catgcgctcg aacaaaatgt cctgcgcacc atgccagaga accagttttc
    11041 cgaaggcttc cagattttcg cgcccgtcct ccacggtgag aaaatgattg cctgccagtt
    11101 gccccatctt gctggcgaag cgcgaactgc cgtaaacgaa caatatctcc gtctcgctga
    11161 accctccggg atagaacagg aggtcaccgc gagatccatg actggtgtga ttctcgaatt
    11221 ccagccccgt atccatggcg cccaggggaa tccagaccgc ttcgccgctc catcgcgagt
    11281 gaattacctt gttcctgaac ggcaacaatt tcgcgaactt tgcgcaggtc gccgacgcct
    11341 tttcgatttc catgcgcgca acaaagacga atggcccggc ggtaatcttt atcctgttct
    11401 tcacaaatcg cttccctgat cagtgttttg ctgccgcaat cgcctccctg taacaggcac
    11461 cggtccgaca cccgatccgg attcccgccg atgttcggtt cgcttcccgc ttgtcagatc
    11521 tttcccgatt ccccgcgggt caagtattcc cccatcccgg cacggccaag ccacccggtc
    11581 ccatcgacaa tgcactgccc ccgcagaaaa acctttttca cccggcccgt caccggccgg
    11641 ccctcgaaca gggtgtaatc gacccggctg tgctgttccg ccgcgcggat agtccactgc
    11701 tcgtcaggat cgaacagcac gatgtccccg tccgatccca ccgcgatcgc gcccttgcgc
    11761 gggaacagac cgaacagctt ggccggtgcc gtcgaggtga ttccgacgaa gcgattgatc
    11821 gacatgccct gctttttgac ggcgccgtcg aagatgaggg gcaatcgggt ctcgactccg
    11881 ggcgcaccgt tggggatttt gctgaaactg tccgcgcctt gctgcttgga gaacttcatc
    11941 ccataaggct tttcgttgaa gcagaacggg caatggtcgg tcgataccac ctgcaggtcg
    12001 tcggtcttca gaccgcgcca gaggtgctgc tggtggttgt gatcgcgtaa tggcggtgtc
    12061 atgacgtact tcgcgccttc gaacccgggc cggtcgtaat cctgcgcagt caggaacagg
    12121 taatgcggac aggtttccgc atggacggga attccgcggt cgcgcgcttc cgtcaccgct
    12181 gacagcgctt cgcccgcgga caggtgcacg atataaagcg gagtgcccgc aagctccgca
    12241 aggcaaatcg cccgatgtgt ggcctcccct tcgagtatcg acgggcgggt ggtggcgtgg
    12301 tacttgggag cgcgtagtcc ctgcgccact gcttcctgga ccagaacctg aatgaccggg
    12361 ccgttctcgg cgtgaataca ggtcatggca ccgttggcac ccgccacgcg catggccttg
    12421 aacagcgcgc catcgtccac catcagtacg ccggggtagg ccatgaaaag tttgaagctg
    12481 gtcacaccct cacgcctgac cagcgttttc atatcggcca ggctctgctc gttgacgtcg
    12541 agcatgatca tgtgggcgcc gacatccacg caggcgctgg cgcgccgctt gtgccagtcc
    12601 tcgagcccga ccaggggact ctttccggta gtctggttgc aaaagtcgat gagagtcgtc
    12661 gttccgccaa aagccgcggc tttcgttccg gtttcaaaag tgtccactgt gtgtgtcgga
    12721 ccgaactccc agtccaggtg ggtatgtacg tcaacgccgc cgggcaggac caggaggccg
    12781 ttcgcgtcat gcaccttgac gtccgcgccc acggcaatgt tcctgccgat ggtctggatt
    12841 cggccgtcga cgatcacgat atcggcggtg taatcatcca ccgccgtcac gatcctcccg
    12901 ttttttatca gggtcgcagc catctgcttc tcctccattg ataggtttgc tccgacatat
    12961 tccgttctcg tagagtcaag gcgcaagcgc ggctaccgtt tctgcaatcc gcgtccgggt
    13021 ctttgcgctc gacagcaaca ccatcagcaa gagccttgcc ttggggccct tcagatcacc
    13081 cgcaaagatt ccgcccgcat cggcgaggtc gcggccaccg ccacccccgc cgtagactgg
    13141 ctgcacccgg cccacgggac agcgggaagt gatcagaacg ggaacgttct tccgcgtagc
    13201 cagccgcacg agttcggcca accgcgacgg gccattgccg cgcccgaatc cctcgatgac
    13261 caaacccgct acgcccctct cgagggcggc ctccaggccg cgcaggtcga ctccaagggt
    13321 gagtcggaac agctctacgc gttcttcgag ctgatcgacc ttgaacgaat ggcgcagaac
    13381 cggccgccga tggatgatga cttcggcgcc atcgacctgg cccaaggcac cgtgttcgta
    13441 tgattggaac gtctccaccg cactggcgtg gactttggtc acatctcgcg cagcgtggat
    13501 cgcaccgttg aagcagatca ttgcgccaag gccgcgcgcc gacggcgccg cggcgacccg
    13561 cagggcgtta agcagattga tggggccgtc ggactgggga tcgtcgtgag cgcgctgcgc
    13621 gccggtgtag accgccggct tgtcggaatc gaggagcagg tcggccagat agcaggtctc
    13681 ctccatcgtg tccgtgccat gcgtaacaac tacgcccgcc acctccggcc cgccgagcat
    13741 ctctttgatg cgcttggcta gatcgaaagc gaactgcacg ttcatttcat aactcggaat
    13801 tgtcgcaatg tcgtggatct cgatctcggc aaaatcggcc acctggggca gcatcctgag
    13861 cagctcatcg gcgcgctggg aggcaacggt gctgcccaac ttcgggtcat agcgcgacgc
    13921 aatggttccg ccggtggcca acagcacgat cttaggtttc atgccgcgat ccctgttaat
    13981 tggcggcccg cgctttttcg agcatgacgt tcagcaacac gttgcagccc gcagcgaggt
    14041 cttccgcctt ggcgttctcg atctcgttgt gactgatgcc gccttcgcag ggcacgaaaa
    14101 tcatgctggt aggcgtaact cgcgccatgt agatggcgtc gtggccagct ccgctgacga
    14161 tgtccatgta gctcaggccc aaggtctgcg ccgcattgcg caccgcggat acacatccgc
    14221 tgtcgaaatt gcaggccggg taattagcga cgcgctcgac gctcgagttc acatttccgg
    14281 tctgcgcaag ccgctcgcat atggcgcgca tctcgccttc catacggtcg agtgccgcgt
    14341 cttgcgcgtg ccgcaggtcc accgagaagc gcacctcacc cggaatgacg ttgcgcgagt
    14401 ttggcttgac ctgcatgaat cccactgtgc ctcgaccgtc aggctggtag cggaaggcga
    14461 gcgcgttgat ttcggcaacc atcttcgaag cggcaaccaa ggcgtccttt ctcagccgca
    14521 ttggcgtggg cccggcgtgc gcgtcctgcc cggtaacgac gacgtcgtac cagcgcaagc
    14581 cgagcgcgcc gaccacgacc ccgatcacct tgcgcgtatc ctccagaatt ggtccctgtt
    14641 cgatgtgcgc ctcgaagtag gcgcctacgg gccggccgcc gattttttcc tttccggcgt
    14701 acccgatctg ctccagcgcc tcgcccaccg tcactccctc caggtcggcg cgctgcaggg
    14761 tggaatcgag cgcaaaggcg cccacgaata cccccgagcc catcatcacc ggggtgaagc
    14821 gcgacccttc ctcattggtc cacaccgcca cttcgaccgg cgcttctgtg ctgatcttgt
    14881 gatcattgag ggtgcgaatc acctccagcc ctgccatcac gccgtagttg ccgtcgaact
    14941 tgccgcccga aggttgcgtg tcgatgtgac tcccggtgat gatcggcgga agaccgttgt
    15001 ccttgcccgg acgccgggcg aagatgttgc cgataccatc tatcgcgacg ctgcagcctg
    15061 cgtccttgca ccagcaaatg aaaaggtcgc gcgcttcgcc gtccagcctg gaaagagcga
    15121 gccggcgcac gccgcctttt tccgtggcgc cgatcctggc gagatccatc agggacttcc
    15181 acagtcgttg cgagtcaatc gatattgcag ccatacctga ccaccttgtg tgagccgcgc
    15241 cctgcgacgg cggggagcgg aatgagcgga gcgattttcc tcacttgagc aaaccggcaa
    15301 ccggcttgcc tttttcggcc tgcggagtgg gattgttggg atgagtgctc cagcttccgt
    15361 atccctggac cgcccgcccc gtccgcgggt cgatagaccc gatcgcgagt tctttcaagg
    15421 tgatgcaatt ctccactgga cagacgttca cgcacaggtt gcagcctacg cactcctcgt
    15481 cgatgacctc gaagtgacgc cggccgtcct tctcgttgcg gatggactga tgcgacgtat
    15541 cttcacaggc gatgtagcag cggccgcact tgatgcacag atcctggtcg atgtgcgcct
    15601 tgaccgtata gttcagattc aggtgttccc aatcgacgaa gttgggcacg gctttgccaa
    15661 cgaaggcgga gagtgtccga tgtcccttct cgtccatcca gttgttcagg ccgtcgatca
    15721 tgtcttccac caccttgaag ccgtaaacca tcgccgcggt gcagacctgg acgttgtgcg
    15781 cgccgagcgc catgaactcg gcggcatcgc gccaggtgga gatgccgcca atgccggaga
    15841 tcgacattcc ggccgactcg cggtcgcggg cgatctcgcc gaccatgcgc agcgcgatcg
    15901 gcttgatcgc cggtccgcag taccctccgt gcgcgccctt gccatccacg ctcgggctgg
    15961 gagacatcag gtcgaggtcc acgcccatga tggagctgat ggtgttgacc agcgaaactg
    16021 catccgctcc gccgcgcagc gcggcgcggg ccggttgcag aatgttggtg acgttgggcg
    16081 tgagcttgac gatcactggc aggtgcgtgt gttccttgca ccagcgcgtg accatctcga
    16141 cgtactcggg aacctggccc accgccgagc ccatgccgcg ctccgacatg ccatgggggc
    16201 agccgaagtt cagctcgatg ccgtcggcac cggtatcctc gaccatcggc aggatccgct
    16261 tccatgcctg ctcttcgcac ggcaccatca gtgacaccgc gagcgcccgg tcgggccaat
    16321 cgcgcttgac ctggcggatc tcgcgcaggt tgtcgtccag catccgatcg gtgatcagtt
    16381 cgatgttgtt gaaacccacc aggttgcggg cggtacccag cagcgcgccg taacgcgggc
    16441 cgctgacgtt gacgatcgga gggtcttcgc cgagcgtctt ccacaccacc ccgccccatc
    16501 cggcctcgaa cgcccgattg acgttgtagg ccttgtccgt cggcggcgcg gatgcgagcc
    16561 agaacggatt gggtgaatgg atgcccacga agttgcagcg cagatcagcc atgtcgcgcc
    16621 tccgttttcg aaaccgcgcg cagatgccgg tcgatcgaca aggcagcgcg cttgccgtcc
    16681 tcgaccgcca ccacggtgag gtcctgcccg cctgccacac agtcgccgcc ggcccagaca
    16741 ccgggaatgg aagttctgcg ttccgcatcg acggcaatcc ttccggattt catttcgagc
    16801 acatcggcgt tgccgtccag agcgtccttg atcagcattt gtccgatcgc tttgaagacc
    16861 atgtccgctg caacgatgaa tttgtcgccg ccacccgcga ggcgtccctg actgtcgagc
    16921 cgcgtttgct cgaactccac cgcggtgagc acgccgtcgc gggccagcag ccgaaggggc
    16981 cgcgcccaat gctttatgcg cactccgctg gtctgtgccc aggcctgctc cttgtgactg
    17041 gcgcccatgc tttgcgggcc gcgacggtag acgatggtca cgtcttccgc acccagccgc
    17101 ttgacctggc tggcgatgtc gatcgcggtc atcccgccgc cgattacgac cacgccgtgg
    17161 cccaccggca tctggctcag gtcccgtgat tgccgaagtt tctcgatgta ggcgatggca
    17221 tcgaacacgc cggcgggcgg ctcatcgaca ctgagtgcat tcactccgcc cagaccgata
    17281 cccaggaaca ccgcgtcgta atcctgccgc agccgtgaca acacgatgtc ccgcccgagc
    17341 cgctgccgca gccggttctc gataccaccg atctccagca gatactcgac ctcgcgctgc
    17401 gcaatgaggc cggtgacctt gtaggcggcg acgccatact cgttcaatcc acccagcttg
    17461 tcgcgcgcct cgaataccgt gaccgagtgg ccgagcatgg aaagccggtg cgcgcacgaa
    17521 agccccgcag gcccgccgcc gaccacggcg atccgcctgc cggtttcggg cccgcgctcg
    17581 aacagctgaa tgcgccgatc gaacagccaa tcggtcgcgt ggcgctgcaa tgccccgatc
    17641 gccaccggct tgtgctcttg atcgttgcgc acgcaggcac cttcgcacag cacttcggtc
    17701 gggcagacgc gggcacacac cgcacccatg atgttctgtt tgaggatgtc gtgggccgcg
    17761 cccttgacgt tgctggtcgc gatcttgcgg atgaaactgg ggatgtcgat ctgcgtcggg
    17821 cacgcccgga tacagggcgc gtcatggcag aagtagcagc gatcgctttc gatcagcgcc
    17881 tgattgggtg ccaggggcgg gtgtgcgtcc gcgaagttcc ttgcgtactc ctccgcagcg
    17941 aggcgcccgc cgcggatgtc gctcttggaa tcgcgatcgc tcattttgcc c
//
LOCUS       DASQOP010000005         6234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_202957, whole genome shotgun sequence.
ACCESSION   DASQOP010000005 DASQOP010000000
VERSION     DASQOP010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6234
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202957"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..105
                     /locus_tag="VE030_00190"
     CDS             <1..105
                     /locus_tag="VE030_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF21862.1"
                     /translation="TLIGALRKRGGKRGVASLCIGGGEATAMAIELVA"
     gene            327..1235
                     /locus_tag="VE030_00195"
     CDS             327..1235
                     /locus_tag="VE030_00195"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZF21863.1"
                     /translation="MANKKIGFIGVGMMGHGMAKNLVEKGFPTTILGHKNRAPVEDLV
                     KRGAKEAKDVAVLVAASDIVFICVTGSSQVEDLVYRKGGILESARPGQIVVDTSTSQP
                     GSTLKIVGEFQSRDVRFVDAPLTRTPVEAEQGRLNTMVGADAATFKEIEPVLNAFCEN
                     IFHIGDAGAGHKIKLINNCAAIGQMALIAEALTACAKLGVDPKKYFQLVSTGAANSGI
                     FQMLAGKAIEGDFTGMKFGLANALKDVRYYNQMAMEFGMAGSMAAATLQTLTHAVNLG
                     FGGPEHLVASLVPAIAKLNSTPFPPE"
     gene            1478..2080
                     /locus_tag="VE030_00200"
     CDS             1478..2080
                     /locus_tag="VE030_00200"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HZF21864.1"
                     /translation="MANAIQFYFDFASPYGYMAATQISRLAAKHGRSVEWKPFLLGVV
                     FKVTGGVPLPNAPLKGDYSRRDMARCARLYGVPFKVPSKFPIASQAPARTVYWLEPQG
                     SAIQEAAVLALYRAYMVDDRDISSPEVTADVAGSIGLDRQKVLDVIADPVMKERLKAE
                     TETAIQRGVFGSPYMIVDDEPFWGVDRVEHLDKWLATGGW"
     gene            2111..2755
                     /locus_tag="VE030_00205"
     CDS             2111..2755
                     /locus_tag="VE030_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HZF21865.1"
                     /translation="MAEYTLYGFAQSGNSYKPALYLELAGADWAPRFVDFFNGETRTP
                     AYREINVMGEAPVLEHRGKRMSQSGVILDYLVEQFRQFGWTNDAERREVLRWLFWDNH
                     KLTSYTATYRFLRQFAPDTGKAVVENFLKRSESAWKVLDTHLAGRSFVVGERPTIADL
                     SICAYLFFDDEIGVDWNQYPNIRDWLARIRALPRWRHPYQLLPGHPIPDKKSGS"
     gene            2752..4359
                     /locus_tag="VE030_00210"
     CDS             2752..4359
                     /locus_tag="VE030_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HZF21866.1"
                     /translation="MTAIRSKLNPRTPDYKASQGAMAALVADLSAKVGLINQGGGEAA
                     REKHLSRGKLLPRERIRRLLDVGSPFLELSQLAAWNMYDGAVPAAGIITGVGRISGRE
                     CVIVANDATVKGGTYFPMTVKKHVRAQEIAQQNRLPCIYLVDSGGANLPTWDEVFPDR
                     DHFGRIFYNQANMSAAGIPQIACVMGSCTAGGAYVPAMADEAIIVRNQGTIFLAGPPL
                     VRAATGEVVSAEELGGADVHARTSGVADHYALNDPHALELARRIVSHLNLKKEIALEI
                     REPREPLYEVAELHGVVPTDARKPFDVREVIARIADGSELDEFKPLYGTTLVCGFAHI
                     FGYPVGILANNGILFSESAMKGAHFIELCAQRRIPLVFLQNITGFMVGKKYEAGGIAK
                     DGAKLVTAVSCARVPKFTVIIGGSFGAGNYGMCGRAYGPRFLWMWPNARISVMGGEQA
                     AAVLAQVKRDGLEKSGKSWSREDEEAFKAPIRAQYETQGHPYYASARLWDDGVIDPAD
                     TRMVLGLGISAALNAPIEETRFGVFRM"
     gene            4363..5541
                     /locus_tag="VE030_00215"
     CDS             4363..5541
                     /locus_tag="VE030_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZF21867.1"
                     /translation="MDARFPHSDKESIYLTEEHVALRDQVARFLAKEVEPHGEAWEEQ
                     GFVPREVLRKMGELGFFGITYPAEYGGAEADALTALVFAEAVSRTTFGGFAATVLVHT
                     DMASPHLVNAGSRTQLGKYLPAIIRGERITAVAVTEPDAGSDVAAIRTRARRDGNGWL
                     LNGAKMFITNGVHADLYFVAARTDSEAKGSRGISMFIVEKGVPGFRVGRALKKTGWLC
                     SDTAELVFEDCRVPAENLLGEENAGFYAVMKNFQTERIAAAAIAVGHALTALRLTVEY
                     VKSRKAFGGVLFDKQVIRQRLAMLEAKTHAARQFLYHCGWLAAQRRDCVREVSMLKAL
                     TGELVNEVLYTCQQFHGGMGYMRESAVERLARDSRVLSIGGGATEVMLEEVAKRFAQD
                     "
     gene            5684..>6234
                     /locus_tag="VE030_00220"
     CDS             5684..>6234
                     /locus_tag="VE030_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019704017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZF21868.1"
                     /translation="MTTTQALQNEIQRGIATVRMNRPDVHNAFDDHLIAELTTELRRL
                     DQAPEVRVVVLAASGKSFSAGADLNWMQRMAKYSEAENLRDAVAMAGLMRTLHGMHKP
                     TIARVQGAAFGGGVGLVACCDIAVAAAEASFSLSEVRLGLIPSVISPYVIAAIGEREA
                     RRYFLTAERFGAEEARRIGLVHEV"
BASE COUNT         1184 a         1872 c         2088 g         1090 t
ORIGIN      
        1 acgctgatcg gcgcactgcg caagcgtggc ggcaaacgcg gcgtcgcgtc gctctgcatc
       61 ggcggcggcg aagccaccgc catggccatc gaattggtcg catagcgacc aaggaaaatg
      121 cattcaacgc aaaggacgca aaggtacgcg aaggaaaagc gaaacactgg aaacgagaga
      181 aattgcgccg gcgagtgaca tagattggtc gctcttcact gttgataatc tgggacgtgt
      241 atgccagaac tattcttctt gttctccttt gcgtaccttt gcgtcctttg cgttgagcgt
      301 ggtttgttct ttcaggagag agtgtgatgg cgaacaagaa aatagggttc atcggcgtgg
      361 gcatgatggg ccacggcatg gcgaagaacc tggtcgagaa gggtttcccg accactatcc
      421 tcggccacaa gaaccgcgcg ccggtcgaag acctggtgaa gcgcggggca aaggaagcga
      481 aggatgttgc cgtcctggtc gccgccagcg acatcgtgtt catctgcgtg accggctcgt
      541 cgcaggtgga agacctggtc tatcgcaagg gcggaatcct ggagtcggcg cgcccggggc
      601 agatcgtggt ggacacctcg accagccagc cgggttcgac gctgaagatc gtcggcgaat
      661 ttcaatccag ggacgtgcgc tttgtcgatg cgccgctgac ccgcacgccg gtcgaagcgg
      721 aacagggacg gctcaacacc atggtcgggg ctgacgcggc cacgttcaag gagatcgagc
      781 cggtgctcaa cgcattctgc gagaacattt tccacatcgg cgacgccggc gcgggtcaca
      841 agatcaagct gatcaacaat tgcgcggcga tcggacagat ggcgctgatc gcggaagcgc
      901 tgacggcgtg cgcgaagctc ggcgtggatc cgaagaagta cttccagctg gtttcgaccg
      961 gcgcagcaaa ctcgggcatt ttccagatgc tggcgggcaa ggcgatcgaa ggcgatttca
     1021 ccgggatgaa gttcgggctc gccaatgcac tgaaggacgt gcgttactac aaccagatgg
     1081 ccatggagtt cggtatggca ggttcgatgg cggcagcgac gctgcagact ttgacgcatg
     1141 cggtgaatct cggcttcggc ggtcccgagc atctggtcgc gtcgctggtc cccgcgatcg
     1201 ctaagctcaa cagcacgccg ttcccgcctg aataaagaca tgttcaccac gaaggcacga
     1261 agacacgaag aagagcagaa cggacaatgg ggcaaggtct gagccatcgc gacgacgccg
     1321 gcaatggcgc gttcggccga gacgtgggcg tacgtcgatt cggcattcgc gggcctgttc
     1381 ggtgcatttc ttgctcgcct tcggttttct tcgtgtcttc gtgccttcgt ggtgagattg
     1441 aaggtttttc tccaaccggc aaaccaaagg gcaagtgatg gccaatgcga tccagtttta
     1501 cttcgacttc gcttcgccct acgggtacat ggcggcgacg caaatttcac gcctcgcggc
     1561 gaagcacggc cgcagcgtcg agtggaagcc attcctgctc ggtgtcgtgt tcaaggtgac
     1621 cggcggcgtg ccgctgccca acgcgccgct gaaaggcgac tacagcaggc gcgacatggc
     1681 gcgctgcgcg cgcctgtacg gggtaccgtt caaggtgccg agcaagtttc cgattgccag
     1741 ccaggcgccg gcgcgcaccg tctattggct ggagccgcag ggatccgcca ttcaggaagc
     1801 ggccgtgctc gcgctctacc gtgcctacat ggtggacgac cgggacatct ccagtccgga
     1861 ggtcactgcc gacgtggcgg gatcgatcgg cctggacagg cagaaggtgc tcgacgtcat
     1921 cgccgacccg gtgatgaagg agcgtctcaa ggccgagact gagaccgcga tccagcgcgg
     1981 cgtgttcggc tcgccttaca tgatcgtgga cgatgagccg ttctggggcg ttgacagggt
     2041 ggagcacctg gacaaatggc ttgcgaccgg gggctggtga ccggcgtacc cacatccgga
     2101 tcagaacatc atggccgaat acaccctgta cggcttcgcg cagtccggta actcgtacaa
     2161 gccggcgctg tacctcgaac tcgccggggc cgactgggcg ccccgcttcg tggatttctt
     2221 caacggcgaa acgcggacgc cggcgtaccg cgagatcaat gtcatgggcg aagcgccggt
     2281 gctcgagcac cgcggcaagc gcatgagcca gtccggcgtc attctcgact acctggtcga
     2341 acagttccgc cagttcggct ggacaaacga cgcggagcgc cgcgaagtcc tgcgctggtt
     2401 gttctgggac aaccacaagc tcaccagcta tacggcgacc tatcgtttcc tgcgccagtt
     2461 tgcaccggac accggcaagg ccgtcgtcga gaacttcctc aagcgttcgg aaagcgcctg
     2521 gaaggtgctg gatacgcacc ttgccggccg cagcttcgtg gtcggcgaac ggccgaccat
     2581 cgccgacctg tcgatttgcg cgtacctgtt cttcgacgac gagatcggcg tcgactggaa
     2641 ccagtatccg aacatccgcg actggctcgc acgcatccgc gccttgccgc gctggagaca
     2701 tccgtatcaa ctcctgcccg gccacccgat tccggacaag aagtcggggt catgacggcg
     2761 atccgcagca agctcaatcc gcgcacgcct gattacaaag ccagtcaggg ggcgatggcg
     2821 gcgctggtgg ccgacctgag cgccaaggtc gggctaatca accagggtgg cggcgaggcg
     2881 gcgcgggaga aacacctcag ccgcggcaag ctgctgccgc gcgagcgcat ccggcgcttg
     2941 ctcgatgttg gctcgccttt tcttgagttg tcgcagcttg ctgcgtggaa catgtacgac
     3001 ggcgcggtgc cggccgccgg catcattacc ggcgtggggc ggatatcggg gcgcgagtgc
     3061 gtcatcgttg ccaacgacgc cacggtgaag ggcggcactt attttccgat gacggtgaag
     3121 aagcacgtgc gggcgcagga gatcgcgcag cagaaccgct tgccgtgcat atacctggtc
     3181 gattctggtg gcgcgaacct gcccacctgg gacgaggtgt tccccgaccg cgatcatttc
     3241 gggcggatct tctacaacca ggcgaacatg tcggcagcgg ggattccgca gatcgcctgc
     3301 gtgatgggtt cgtgcaccgc aggcggtgcc tacgtgccgg cgatggcgga cgaagcgatc
     3361 atcgtcagga accagggcac catctttctg gccgggccgc cgctggtcag ggccgcgacc
     3421 ggcgaagtgg tgagcgccga ggagctgggc ggcgcggatg tacatgcccg cacttccggc
     3481 gtcgctgacc actacgcact caacgacccg cacgcactgg aactggcgcg ccgcatcgtt
     3541 tcgcacctca acctgaaaaa ggagatcgcg ctcgagatcc gcgagccgcg cgagccgctt
     3601 tacgaggtgg ccgaactgca cggcgtggtg cccaccgatg cacgcaaacc gttcgacgtg
     3661 cgcgaagtga ttgcccggat cgcggacggc agcgaactcg acgaattcaa acctctctac
     3721 ggcaccacac tggtgtgtgg cttcgcgcac atcttcggct atccggtcgg aatccttgcc
     3781 aacaacggca tcctgttctc cgagtctgcg atgaagggcg cacatttcat cgagctgtgt
     3841 gcgcagcgcc gcatcccgct ggttttcctg cagaacatca ccggcttcat ggtgggcaag
     3901 aagtacgaag ccggcggcat cgccaaggac ggcgccaaac tggtgaccgc agtgtcgtgt
     3961 gcccgcgtgc cgaagttcac ggtgatcatc ggcggcagct tcggcgccgg caattacggc
     4021 atgtgcggcc gagcctatgg cccgcgtttc ctgtggatgt ggccgaacgc gcgcatctcg
     4081 gtgatgggcg gcgaacaggc cgcagccgtg ctggcgcagg tcaagcgcga tggcctggag
     4141 aagtccggca agtcgtggag ccgggaagac gaggaagcgt tcaaggcgcc gatccgcgcg
     4201 cagtacgaga cccaggggca tccgtactat gccagcgcgc gcttgtggga cgacggcgta
     4261 atcgatcccg ccgacacccg catggtgctg ggactgggaa tctccgccgc gcttaacgcg
     4321 ccgatcgagg aaacgcgctt cggcgtgttc cggatgtaga gcatggacgc gcgtttccct
     4381 cacagcgaca aggagtctat ctacctcacc gaggagcatg tcgccctgcg cgatcaggtc
     4441 gcgcggtttc tggcgaaaga agtcgagccg catggggagg catgggaaga acagggcttt
     4501 gtcccgcgcg aggttctgcg caagatgggc gagctggggt tctttggcat tacctatccg
     4561 gccgagtacg gcggcgcaga ggcggacgcg ctgaccgcgc tggtgttcgc cgaagcggtt
     4621 tcgcggacga ctttcggcgg tttcgcagcg accgtgctgg tacataccga catggcttcg
     4681 ccgcacctgg tcaacgcggg ctccaggaca cagctcggca agtacctgcc cgccatcatc
     4741 cgcggagagc gcatcaccgc ggtcgcggtc accgagccgg atgccggctc cgacgttgcg
     4801 gcaatccgca cgcgcgcccg cagggacggc aacggctggc tgctcaacgg cgccaagatg
     4861 ttcatcacca acggcgtgca cgcggatctc tacttcgtcg cggcgcgcac cgactccgaa
     4921 gccaaggggt cacgcggcat ttcgatgttc atcgtcgaga agggcgtgcc ggggttccgc
     4981 gtcggccgcg cgttgaagaa gaccggctgg ctgtgctccg acaccgcgga actggtgttc
     5041 gaggactgcc gcgtgccggc ggagaacctg ctcggggaag agaatgccgg cttctacgcg
     5101 gtgatgaaga atttccagac cgagcgcatc gccgcggccg ccatagccgt cggccacgcg
     5161 ctgacggcgt tgcgtctgac cgtcgagtac gtcaagagcc gcaaggcctt cggcggggtg
     5221 ctgttcgaca agcaggtgat ccgccagcgc ctggcgatgc tggaggcgaa gacgcacgca
     5281 gcgcgccagt ttctatacca ctgcggatgg ctggctgcgc aacggcgtga ttgcgtacgc
     5341 gaagtgtcga tgctgaaagc ccttaccggc gaactggtga acgaagtgct ctacacctgc
     5401 cagcagttcc atggcggtat gggatacatg cgcgagagcg ccgtcgagcg cctggcgcgg
     5461 gattcgcgtg tgctctccat cggcggcgga gcgaccgaag tgatgctcga ggaagtggcg
     5521 aagcgcttcg cgcaggactg aagaacaccc tctccccggc cctcccccgt caagggggag
     5581 ggagaaaaat acctcaggac cgggcgtgga gtcgggaaga gggagggaaa caagattgga
     5641 atttctccaa ttcaatttgg tatcgactgg aagtaagagc cgaatgacga cgacccaggc
     5701 attgcagaac gagatccagc gaggcatcgc caccgtccgg atgaaccgtc cggatgtgca
     5761 caacgccttc gacgaccacc tgatcgcgga attgaccacg gaattgcgcc ggctcgacca
     5821 ggcgccggaa gtgcgggtgg tcgtgctcgc tgccagcggc aagagcttct ccgcgggcgc
     5881 ggacctcaac tggatgcagc gcatggcgaa atattccgag gccgagaacc tgcgtgacgc
     5941 cgtggcgatg gccgggctga tgcgcacgct gcacggaatg cacaaaccga cgattgcgcg
     6001 ggtgcagggt gcggcgttcg gcggcggcgt cgggctggtc gcgtgctgcg acattgcggt
     6061 ggccgccgct gaggcgtcgt tttcattgtc cgaagtgcgg ctggggctga tcccctcggt
     6121 gatcagcccc tacgtcatcg ccgcgatcgg cgagcgcgag gcgcggcgtt attttctgac
     6181 ggccgagcgc ttcggcgccg aggaggccag gcgcatcggc ctggtgcacg aagt
//
LOCUS       DASQOP010000006         2797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2608, whole genome shotgun sequence.
ACCESSION   DASQOP010000006 DASQOP010000000
VERSION     DASQOP010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2608"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VE030_00225"
     CDS             complement(<1..288)
                     /locus_tag="VE030_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZF21869.1"
                     /translation="MVSLVINGKTREVNAAPGTPLLWVLRDSLRMTGTKFGCGIAQCG
                     ACTVHLDGEAVRSCVTPLSAAAGKKVTTIEGIGATRAGKAVQAAWIRLDVPQ"
     gene            501..1073
                     /locus_tag="VE030_00230"
     CDS             501..1073
                     /locus_tag="VE030_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HZF21870.1"
                     /translation="MGYLLSVSRIIDGVNERIGRLVYWLVLVMVLVSAGNAASRYMFS
                     VASNAWLELQWYLFAAVFLLCSGYTLRHNEHIRIDVIAGNLSRRAQMWIDIFGTLFFL
                     LPMAAFVTWLSWPIFMNAWNSNEISGSAGGLIRWPARLLVPVGFFLLSLQGASELIKR
                     IAFLKGLIPDPLEKHEVPLELAIARDEAAK"
     gene            1070..2623
                     /locus_tag="VE030_00235"
     CDS             1070..2623
                     /locus_tag="VE030_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HZF21871.1"
                     /translation="MTQFLIANMAPLMFAALVVFLLFGYPVSFALAAIGLSFGFLGIE
                     LGLLTPALFGALPERIFGIMSNDTLLAVPFFTFMGLILERSGMAEDLLNTIGQLFGPI
                     RGGLAYAVIFVGALLAATTGVVAASVISMGLISLPIMLRYGYDRRLASGVIAASGTLA
                     QIIPPSLVLIVLADQLGKSVGDMYAGAIFPGLVLTALYAIYIFGVTLIYPKAAPALPP
                     EARTLRGAKLLARVLLVLIPPLALIFLVLGTIFLGVATPTEGGAMGATGALVLAMINR
                     RLSWSLLRQAMNSTAKLTSFVVFILVGSTVFSLTFRGVNGDLWVEHLLTGLPGGAIGF
                     LIVVNILVFLLAFFLDFFELAFIIVPLLGPVADKLGIDLIWFGVLLGVNMQTSFMHPP
                     FGFALFYLRSVAPGREYLDKLTAKLTPPVTTGQIYWGAVPFVVIQVIMVAIVIGFPGL
                     VTGNLVKSSGDPSKIQTFVPADSEGTQDQTPPDFGSPGGAKSDAERKSGEGARDKREA
                     DDLENLFKK"
BASE COUNT          445 a          910 c          860 g          582 t
ORIGIN      
        1 ttgcgggacg tcgagccgga tccacgcggc ctgtaccgcc ttgccggccc gagtggcccc
       61 gatcccctcg atggtggtca ccttcttgcc ggccgccgcg gacagcggcg tgacgcagga
      121 gcgcacggcc tcgccgtcga ggtgcacggt gcaggcaccg cactgagcga tgccgcagcc
      181 gaacttcgtg ccggtcatgc gcagcgagtc gcgcagcacc cacaagaggg gcgtgccggg
      241 cgcggcgttg acctcgcggg tcttgccgtt gataacgagg gaaaccatga aaaacctcct
      301 aagatggacg cctggatgag cttgctggtc ggctttgatc ttattccggc ccccggagga
      361 agtccatacg gaaacaccgc aggcgcaaga cccgagaatg cattatcttg cctagatccc
      421 gtgagccgct tgcgacggcg ctccgttttt gccaaacccg ggggcaatag gggacaatcg
      481 cacgccactg gggagggaaa atgggctacc tgctgtcggt ttcgcgcatc atcgatggcg
      541 tgaacgagcg catcggacgg cttgtgtact ggctggtgct cgtcatggtg ctggtaagcg
      601 ccggcaacgc ggcctcccgc tacatgttct ccgtcgcctc gaacgcgtgg ctcgagctgc
      661 agtggtatct gttcgccgcg gtcttcctgc tctgttccgg atacacgctg cggcacaacg
      721 aacacatccg gatcgacgtg atcgccggca acctgtcccg ccgcgcgcag atgtggatcg
      781 acatcttcgg caccctcttc ttcctgctgc ctatggcggc ctttgtcacg tggctctcct
      841 ggccgatatt catgaacgcc tggaacagca acgagatctc cggcagcgca ggcggcctga
      901 tccgctggcc ggcgcgcctt ctggttccgg tgggcttctt tctgctgtcg ctgcagggcg
      961 cctcggagct gatcaaacgc atcgccttcc tgaagggcct gattccagat ccgctggaaa
     1021 agcacgaggt cccgctggaa ttggccatcg cgcgcgacga agccgctaaa tgacgcaatt
     1081 tctcatcgcc aacatggcgc cgctgatgtt tgcggcgctc gttgtgttcc tcctgttcgg
     1141 ctatccggtt tcgttcgcgc tggcggcgat cggcctgtcg ttcggctttc tcggcatcga
     1201 gctgggtttg ctcacgccgg cgctctttgg ggcgttgccg gaacggattt tcggcatcat
     1261 gtcgaacgat acgctgctcg cggtgccgtt cttcaccttc atggggctga tcctcgagag
     1321 gagcggcatg gccgaggacc tgctcaacac catcggacag ctcttcggtc ccatccgggg
     1381 ggggctcgcc tatgccgtga tcttcgtcgg cgcgctgctt gccgcgacca ccggcgtggt
     1441 ggctgcttcg gtcatctcga tgggactgat ctctcttccc atcatgctgc gctacggcta
     1501 cgaccggcgc ctcgcgagcg gcgtcatcgc cgcatcgggg acgctggcgc agatcattcc
     1561 gccatccctg gtcctcatcg tgctcgcgga tcagctcggg aaatccgtcg gcgacatgta
     1621 cgccggggcg atctttcccg ggctcgttct caccgcgctg tacgcgatct atatcttcgg
     1681 cgtcacgctg atctatccaa aggccgcgcc ggcactgccg ccggaagcgc gcacgcttcg
     1741 cggcgccaaa ttgctcgcgc gcgtgttgct ggtcctgatc ccgccgctcg ctctgatctt
     1801 cctcgttctc ggcaccattt tcctcggcgt ggccacgccg acggaaggcg gcgccatggg
     1861 cgccaccgga gccctggtgc tggcaatgat caaccggcgc ctcagctggt cgctgctcag
     1921 gcaggcgatg aattcgacgg cgaagcttac ctcgttcgtg gtgttcatcc tggtcggctc
     1981 gacggttttc agcctgacct tccgcggcgt caacggcgat ctctgggtcg agcaccttct
     2041 cacggggctc ccgggcggcg caatcggctt cctgatcgtg gtgaacatcc tggttttcct
     2101 cctcgccttt ttcctcgatt tcttcgagct ggcgttcatc atcgtgccgc tgctcgggcc
     2161 ggtcgcggac aagctcggca tcgacctgat ctggttcggc gtgctgctcg gtgtgaacat
     2221 gcagacttcc ttcatgcatc cgccgttcgg ttttgctctg ttctacttgc gcagcgtcgc
     2281 gcccggcagg gagtacctcg acaaactgac cgccaagctg acgcctccgg tcaccaccgg
     2341 ccagatctat tggggcgcgg tgcccttcgt ggtgatccag gtgatcatgg tcgccatcgt
     2401 catcggcttc cccgggctgg ttaccggaaa cctggtgaag agcagcggcg atccctccaa
     2461 gattcagacc tttgtccccg cggacagcga gggtactcaa gaccagacgc cgcccgactt
     2521 cggttcaccg ggcggcgcga aatccgatgc ggagcggaaa tcaggcgaag gcgcaaggga
     2581 caagcgcgaa gccgacgacc tcgagaatct ctttaagaaa taaacggcag cacggccaaa
     2641 aaacaaaccc cgccgaagcg gggtttgcat tgcagccgtc cgcgctcgcc ggagctacga
     2701 cttcccggcg ccgagcttcg aggcaaacga atagttgtcg taggtcgatt cggcgacgcg
     2761 gaaccacagg aactgctcgt cgcggaattt cttccac
//
LOCUS       DASQOP010000007         4225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_204094, whole genome shotgun sequence.
ACCESSION   DASQOP010000007 DASQOP010000000
VERSION     DASQOP010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204094"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(373..840)
                     /locus_tag="VE030_00240"
     CDS             complement(373..840)
                     /locus_tag="VE030_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HZF21872.1"
                     /translation="MTIHFRTGFLRTAVRLSLIAAVTAPSVAPAAESIRVIDPWARAT
                     LPGQKVGGVYMEIISPRDARLTGVASPVAGAAEVHSMTLEGGTMKMRAVKALDLPAGT
                     PVKLAPGGYHVMLFDLKKPLVAGEKVPLTLVIEEAGKRTHKIAVSAIVRTGDQ"
     gene            893..2098
                     /locus_tag="VE030_00245"
     CDS             893..2098
                     /locus_tag="VE030_00245"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HZF21873.1"
                     /translation="MRNRWEPEDVIYNPFRSSMMRILAALFVWFGSTLAFAQAPLNGA
                     AALGPLASKSYLLVDVLSQQVLAAKNPDARVEPASLTKLMTAYVVFDALRQKRVTLSQ
                     PLMISEKAWRAPGSRMFLDPGKAVAIEDLLRGMIVQSGNDASIALAEGIAGTEEAFVE
                     QMNREAARLGLKNSNFVNATGLPNPQHYSTPADLVLLSAAIIREFPQYFPMYSLHEFR
                     YNNITQRNRNDLLGRDPYVDGLKTGYTESAGFCMIATAQRNGRRLIAVVIDAGSESGR
                     AAEAQRLLNYGFEAFDTVKLYARGQSVHQLPVWKGNSEQLQAGFTDDYYVTIPKGFGD
                     RLKARLESMQPLIAPIRSGDRVGTLRLTFDGQPFGERTVVALQTVGIANLFVRGWHSL
                     RLLARRFGD"
     gene            2313..3167
                     /locus_tag="VE030_00250"
     CDS             2313..3167
                     /locus_tag="VE030_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HZF21874.1"
                     /translation="MIYLNGEFMPIEQARIPVLDRGFIFGDGVYEVIPVYSRHPFRLA
                     GHLRRLQDSLDGIRLANPHDAAEWTRLVRRLIELNEPEDQSLYLQVTRGVAKRDHAFP
                     KGVAPTVFMMSNALSTPPREQVEKGVGAVTAADNRWLRCDIKAIALLPNVLLRQLAVD
                     AGCVEAVLLRDGIMTEGAASNIFVVRNGILLAPPKSPLMLPGITYDVVLELAQANGIK
                     HEVRPVPESELRGADEAWLTSSTKEVLAIVKLDGKAVGGGVPGPLFRRMYALYQEYKE
                     RVMRTSGN"
     gene            3449..3712
                     /locus_tag="VE030_00255"
     CDS             3449..3712
                     /locus_tag="VE030_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HZF21875.1"
                     /translation="MENPSLIEYPTDFPIKVMGRREPRLVHTIVGIVQRHAPDFDPAT
                     VEMRTSKKNSYLSVTCTVRATSREQLDALYQELCDHPSVVMVL"
     gene            3755..>4225
                     /gene="lipB"
                     /locus_tag="VE030_00260"
     CDS             3755..>4225
                     /gene="lipB"
                     /locus_tag="VE030_00260"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006407065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016979 - lipoate-protein ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HZF21876.1"
                     /translation="MQTKSLPYTALSTQHSALVVRKLGLSDYEKTWRAMQGFTAARNT
                     GTPDELWLVEHPPVYTLGLNGKAQHLPRTDNGIPIIKVDRGGQITYHGPGQIVVYTLL
                     DLRRRGLGVRALVRKLENAVIELLADYGLEANGRVDAPGVYVAGAKVAALGLRVR"
BASE COUNT          841 a         1317 c         1271 g          796 t
ORIGIN      
        1 ccccagcgag ttccgctcgg cgctgcgcac cggcgtcgca catctttacg gcagaatcga
       61 tgaggcgctc aagcgcacca aggaccccga gactgcgtcg catctgcgcg acatccgttc
      121 gcaactcggg caagtgccct aacgggttaa ggcggtactt accgcggagg acgcagagga
      181 cgcggaggaa aggcaagagc aaggtaatgg gttcgggtaa aaaccttcca ttgactcatc
      241 atcaatcgcg gcgaaagttg gcggcatagg ttcgcgaaca ctcaccctga ttttatcccc
      301 catttgcttt tcctcgtttt tggttttcct ccgcgtcctc cgcgtcctcc gtgttaagtg
      361 ctggtttctg ccttattggt cgcccgtgcg aacgatcgcg ctcactgcaa ttttgtgcgt
      421 gcgcttgccg gcctcctcga tcaccagcgt aagcggcact ttctccccgg caaccagcgg
      481 tttcttcaga tcgaacagca tcacgtggta gccgccgggc gccagtttca ccggcgtgcc
      541 cgcgggcaga tcgagcgctt tgaccgcgcg catcttcatg gtgccgccct ccagcgtcat
      601 cgagtgcact tctgcggcac ccgcgaccgg actggccacc ccggtaagcc gcgcgtcgcg
      661 cgggctgatg atctccatgt aaacgccccc caccttctgg cctggcagcg tggcgcgggc
      721 ccaggggtcg atcacgcgga ttgactccgc cgccggcgcc actgacggag ccgttacggc
      781 ggcaatcaac gacaggcgca ccgcggtccg aaggaagccg gttctgaaat gaattgtcat
      841 ggggctggaa ctgagatcgc gaaaggcgcg cattgttcca gagggcgcgg cgatgcgcaa
      901 tcgctgggag ccggaggacg tgatatataa tccgtttcgc tccagcatga tgagaattct
      961 cgccgcgtta ttcgtctggt tcggcagcac cctggctttt gcccaggctc cgttgaacgg
     1021 cgcagccgcg ctcggaccgc tggcctccaa gtcgtatctg ctggtggacg tgctcagcca
     1081 gcaggtcctg gccgcaaaga atcccgacgc ccgcgtcgaa ccggcgtcgc tcaccaaact
     1141 gatgaccgcc tacgtggtgt tcgacgcttt gcgccagaaa cgcgtcacgc tgtctcaacc
     1201 gctgatgatt tcggaaaagg cctggcgtgc gcccggctcg cgcatgtttc tggacccggg
     1261 caaggccgtc gcgatcgagg acctgctgcg cggcatgatc gtgcagtcgg gcaatgacgc
     1321 gtccatcgcg ctggcggaag gcattgccgg aaccgaggaa gcctttgtcg agcagatgaa
     1381 tcgcgaggct gcgcgcctcg gactgaaaaa ctcgaacttc gtcaatgcga ccgggctgcc
     1441 aaacccgcag cattactcca cacccgccga cctggtgctg ttgtcggcgg cgatcatccg
     1501 cgagtttccg cagtattttc cgatgtacag cttgcacgag ttccgctaca acaacatcac
     1561 ccagcgcaat cgcaatgatc ttctgggccg cgatccctac gtggacggcc tcaagaccgg
     1621 ctataccgaa agcgccggct tctgcatgat cgccaccgcg cagcgcaacg gccggcgcct
     1681 gatcgcggtg gtgatcgacg ccggctcgga aagcggccgc gccgcggaag cgcagagact
     1741 gctcaactac ggattcgagg ccttcgacac ggtgaagctc tacgccaggg ggcaatcggt
     1801 gcatcagttg ccggtatgga aaggcaacag cgaacaattg caggcgggat tcaccgacga
     1861 ttattacgtc actattccga aagggtttgg cgaccgcctc aaggcccggc tggagagcat
     1921 gcaacccctg atcgcgccga tccgctcggg ggacagggtc ggaacgctgc gcctgacttt
     1981 cgacggccag cccttcggcg agcgcactgt cgtcgccctg cagaccgtcg gcatcgccaa
     2041 cctgttcgtg cgcggatggc acagcctgag gttgctggcg cggcgatttg gcgattaagt
     2101 gcttgggtga ttgggcgatt gggcgattgg gcgattgggc gattgggtaa atagtgaacc
     2161 gcaaatcgca taatcactga atgtcccaat aacttaatcc cttgatccca taatcactca
     2221 atccctgaac tttaatcact caatcgctga atcacttaat cgctcaatca cttaatcgct
     2281 caatcactca atcgctcaat cactgccctc caatgattta cctcaacggc gaattcatgc
     2341 cgatcgagca agccaggatt ccggtgctcg atcgcggctt catcttcggc gacggcgtgt
     2401 acgaagtgat cccggtgtac tcgcggcatc cgttccgcct cgccgggcat ctgcggcgat
     2461 tgcaggacag cctcgacggc atccggctcg ccaatcccca tgacgccgcc gaatggacgc
     2521 ggctggtgcg gcgtttgatc gaactgaacg agcccgagga tcagtcgctg tacctgcagg
     2581 tcacccgcgg cgtggcgaag cgcgatcacg ccttccccaa aggcgtggcg ccgaccgtat
     2641 tcatgatgag caatgcgctt tccacgccgc cacgcgagca ggtggaaaag ggcgtgggcg
     2701 cggtcacggc cgccgacaac cgctggctgc gctgcgacat caaggcgatt gcactgctgc
     2761 ccaacgtgct gttgcggcaa ctcgcggtag atgccggctg cgtcgaagcc gtactgttgc
     2821 gcgacggcat catgaccgaa ggcgcggcca gcaatatttt cgtggtcagg aacggcattc
     2881 tgctggcacc gccgaagagc cccctgatgc tgcccggcat cacttacgac gtggtcctgg
     2941 aactggcgca agccaacggc atcaaacacg aggtccgtcc ggttccggag tcggagctgc
     3001 gcggcgcaga cgaggcgtgg ctcacctctt ccaccaagga agtgctggct atcgtcaagc
     3061 tggacggaaa agccgtcggc ggcggcgtac ccggacccct gttccggcgc atgtacgcgc
     3121 tgtaccagga gtacaaggag cgcgtgatgc gcacctccgg aaattgaccc cgaaaacctt
     3181 gaatcttaat cacacagaca aagtccaact ctcaccacgg aggacgcgga ggacacggag
     3241 gaaaaacaga aacgaggaaa aacgaattgg ggataggagc aagatgactg ttcgtgatca
     3301 tcagccgcca atttcgactc tgcggagcat ccgccaattg aagattttta cactcatatg
     3361 ccttttttct cctgtccttc ctccgcgtcc tctgcgtcct ccgcggtaag tgctgatttc
     3421 tccgtggtga aatgctttgg atagcttgat ggaaaacccc tccctcatcg aatatccgac
     3481 cgacttcccg atcaaggtca tgggcaggcg cgagccgcgg ctggtgcaca cgatcgtcgg
     3541 gatcgtgcag cgccacgcgc cggacttcga tccggcgacc gtggaaatgc gtaccagcaa
     3601 gaagaacagc tacctcagcg tcacctgcac cgtgcgcgcc acctcgcgcg aacagctcga
     3661 tgcgctgtac caggagctgt gcgatcatcc gtcagtggtc atggtgctgt aagggccgtg
     3721 acaagtgact ggtgactagc aagggcaaac ccgggtgcaa accaaaagtc tcccatacac
     3781 agcactcagc actcagcact cagcacttgt agtccggaag ctcggcttgt cggactacga
     3841 gaaaacctgg cgcgcgatgc agggcttcac cgcggcgcgc aacaccggca ccccggacga
     3901 gctctggctg gtcgagcacc cgccggtcta cacgcttggt ctcaatggca aagcgcagca
     3961 cctgccgcga acggacaacg gcattcccat catcaaagtg gatcgcggcg ggcagatcac
     4021 ttaccacggt ccggggcaga tcgtcgtcta cacgctgctg gatctgcgcc gtcgcggcct
     4081 gggagtgcgg gcgctggtgc ggaagctgga gaacgcggtg atagaattgc ttgcggacta
     4141 cgggctcgag gcaaacggcc gcgtggacgc gccgggggtg tatgtcgccg gcgcaaaggt
     4201 ggcggcgctg ggtctgcggg tgcgc
//
LOCUS       DASQOP010000008         4753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_406183, whole genome shotgun sequence.
ACCESSION   DASQOP010000008 DASQOP010000000
VERSION     DASQOP010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4753
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406183"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(245..1582)
                     /locus_tag="VE030_00265"
     CDS             complement(245..1582)
                     /locus_tag="VE030_00265"
                     /inference="COORDINATES: protein motif:HMM:NF016462.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZF21877.1"
                     /translation="MRHVKRLALLFSFVSAIPLSALATDELLISAREQLDNGNPQAAY
                     NLLIPLQPERAGDPEYDLVLGSAALAVGKNTEAVFALERVLAAVPNSAPARALIARAY
                     FNLKETEAAKREFENVRKQKVAPEVATAINRFLEAIERIEASERTSIHGFVEAGIGHD
                     SNVNSATADNRVAVPLFGGTIISLAASSVEQEDAFASFGGGVNFQHPLSKRLGLFGGV
                     AYQNKSNHNETDFSTYYYDANLGLSYRLDRDTLTLAAQYNTFYVNNSAVYRNAYRNAS
                     GLTGQWQHDFDSRNQASFFVQYSLLEYPDQEIRNADRYVGGAGYAHAFGRGTVITYLG
                     VYGGVEDEKEDGVPQLGHHLYGARLGAQWNMSEKFALFLNASGERREYGGPDPFFLVD
                     RVDTQYSASTGLIFFPRKNVRVTTQASGTRNDSNIPINAYDRVVYQITLRYDM"
     gene            complement(1841..4096)
                     /locus_tag="VE030_00270"
     CDS             complement(1841..4096)
                     /locus_tag="VE030_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/lysine/ornithine decarboxylase"
                     /protein_id="HZF21878.1"
                     /translation="MKFRFPVVIIDEDFRSENASGLGVRALAKAIEGEGLEVLGVTSY
                     GDLSSFAQQQSRASAFILSIDDEEFGGGTVEETTEALKTLRAFVKELRFRNADIPIYL
                     YGETRTSRHIPNDILRELHGFIHMFEDTPEFVARHIIREARAYLDSLAPPFFRALVHY
                     AQDGSYSWHCPGHSGGVAFLKSPVGQMFHQFFGENMLRADVCNAVDELGQLLDHSGPV
                     ADSERNAARIFNADHCFFVTNGTSTSNKMVWHANVAPDDIVVVDRNCHKSILHAITMT
                     GAIPVFLTPTRNHLGIIGPIPLDEFRPETIQKKIEANPFARVAKRKKPRILTITQSTY
                     DGVVYNVEMLKETLNASIDTLHFDEAWLPHAAFHSFYKNMHAIGMDRPRPKDPLIFAT
                     HSTHKLLAGISQASQILVQESQTRKLDRHVFNEAYLMHTSTSPQYAIIASCDVAAAMM
                     EPPGGTALVEESIVEALDFRRAMRKVDDEFGKDWWFKVWGPDKIAAEGIGSREEWTLR
                     ANEKWHGFGDLAPGFNMLDPIKATVITPGLDMSGKFPRTGIPASIVTRFLAEHGVIVE
                     KTGLYSFFIMFTIGITKGRWNTLVTALQQFKDDYDKNQPMWRILPEFCQKHPRYERMG
                     LRDLCDQIHGMYKSNDVARLTTEMYLSDMQPAMKPSDAFAAMAHRDIDRVDIDNLEGR
                     VTSVLLTPYPPGIPLLIPGERFNSTIVRYLQFARDFNRKFAGFETDIHGLVEEPGEGG
                     VRFFVDCVRQS"
     gene            complement(4190..>4753)
                     /gene="dcd"
                     /locus_tag="VE030_00275"
     CDS             complement(4190..>4753)
                     /gene="dcd"
                     /locus_tag="VE030_00275"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HZF21879.1"
                     /translation="KSDKWIRRMAREQAMIDPFEPGQVRVANGHKIVSYGTSSYGYDI
                     RCSNNFKIFTNINSTMVDPKNFDEKSFVDFKGDVCIIPPNSFALARTVEYFRIPRNVL
                     SICLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYANEGVAQVIFFESD
                     ADDVCETSYRDRGGKYQGQKGVTLPKI"
BASE COUNT          898 a         1376 c         1472 g         1007 t
ORIGIN      
        1 cccattgccg agcgcatcta cgctaccgag atcatggccg attttgcagg cgacactttc
       61 ttccctccgt tcgatcccgg tacatgggta gaggtttcgc gggagatggg cacgaactcc
      121 gaatcgcttc cgcacgactt cgtgatctac cagcgggccg tggtccgccg ttaatcatac
      181 ctcgtagtag ctcgccggtc cccggatgtt ttagagttgt cgccgcccat cgttttcgtt
      241 gctcctacat gtcgtagcgc aaggtgatct ggtagaccac tcggtcgtac gcgttgatcg
      301 gaatattcga gtcgttccgg gtcccgctgg cctgagtggt cacccggaca tttttcctcg
      361 ggaagaagat caaaccggtg gaagcgctgt actgggtgtc tacgcgatcg accaggaaaa
      421 acggatcggg gccgccgtac tcacgccgct cgccgctggc gttcaggaac agcgcgaact
      481 tctcgctcat gttccattgc gctcctagcc gagccccgta taagtggtgt ccgagctgcg
      541 gcacaccatc ctctttttca tcctccacgc cgccgtaaac gcccagatat gtgatcacgg
      601 taccccggcc gaaggcgtgg gcgtaccccg cgccacccac gtaacggtcg gcattacgaa
      661 tttcctgatc cggatactcc agtagactgt actggacgaa gaagctggcc tggttgcggg
      721 aatcgaagtc gtgttgccac tggccggtca agcccgacgc attgcggtag gcgtttctgt
      781 ataccgcgga attgtttacg tagaacgtgt tgtactgcgc ggcgagcgtc aacgtgtccc
      841 tgtcaagccg gtagctcaag ccgaggttgg cgtcgtaata gtacgttgaa aaatcggttt
      901 cgttgtggtt ggacttgttc tggtaggcca caccgccgaa aagcccgagt cttttcgaga
      961 gcggatgctg gaaatttacc cctccgccga aactggcgaa cgcgtcttcc tgctccacgg
     1021 aactggccgc cagggagata attgtgcccc caaatagagg taccgccacc cgattgtcgg
     1081 cggtggcgct gttgacgttg ctgtcatggc cgatgccggc ttcgacaaaa ccgtggatgc
     1141 tggtgcgctc ggatgcctcg atacgctcga tggcctcgag gaagcggttg atggccgtgg
     1201 caacttccgg cgcgaccttc tgcttgcgga cattttcgaa ttcgcgtttg gcggcttcag
     1261 tttccttcag attgaagtat gcgcgggcaa tcaaggcgcg ggcaggcgcg ctgttcggta
     1321 ctgccgcgag aacccgctcc agcgcgaaca ccgcctcggt gttcttaccg accgccagcg
     1381 ccgccgagcc gagaaccaag tcgtattccg ggtcgccggc gcgctccggc tggagcggga
     1441 taaggaggtt gtaagcggct tgcggattgc cgttatcgag ctgctctctg gccgagataa
     1501 gaagctcgtc ggtggccaaa gcgctcagcg gaatagcaga gacgaacgaa aaaagcagcg
     1561 cgaggcgctt cacatgccgc atggaacctc ccgatcgcta tccatatacc gccaacagca
     1621 caacctaaac cacgactttt aaagactttt tgtatggcaa acattaccga acgaggtgcg
     1681 cagcgtcaaa cgctttgcgc gcaagaggca actgctttac cgcgccggcg ttaccaggcc
     1741 ggcggcgaaa acgcacgcta gcttgcggcg ctcccaatga tttgcgagcc atggtcgatt
     1801 cgcaacgagg gttctgctga atcgaagaat cgggagcgcc tcagctctgg cggacgcaat
     1861 ccacgaaaaa gcgcacgccc ccttcaccgg gttcttctac caggccatgg atgtcggtct
     1921 cgaatccggc gaacttgcgg ttgaaatccc gggcgaactg gagataacgc acgatcgtgg
     1981 aattgaatcg ctcacctgga atcagcaacg ggatgcccgg cggataaggc gtcagcaaca
     2041 cgctggttac gcggccctcg aggttgtcga tgtcgacgcg atctatgtcg cggtgcgcca
     2101 tcgcagcgaa cgcatccgag ggcttcatcg ccggctgcat atcggacaga tacatttcgg
     2161 tcgtaaggcg cgctacgtcg ttgctcttgt acatgccgtg gatctggtcg cataggtcgc
     2221 gcagtcccat ccgttcgtag cggggatgct tctggcagaa ctccggcagg atgcgccaca
     2281 tcggctggtt cttgtcgtag tcgtccttga actgctgaag cgccgtcacc agcgtgttcc
     2341 agcggccctt ggtgatgccg atggtgaaca tgatgaagaa cgaataaagg ccggtcttct
     2401 ccacgatcac gccgtgttcg gcgagaaatc tggtgacgat cgaggctgga atgccggtcc
     2461 tggggaactt gcccgacatg tccaggcccg gcgtgatgac cgttgccttg atcggatcga
     2521 gcatgttgaa ccccggtgca agatcgccga agccgtgcca cttctcgttg gccctcagcg
     2581 tccattcctc gcgcgaaccg atcccttccg cggcgatctt gtctggcccc cataccttga
     2641 accaccaatc cttgccgaac tcgtcgtcca ccttgcgcat ggcgcggcgg aaatcgagcg
     2701 cctcgacgat ggattcctcc accagtgccg tgcctcccgg gggttccatc atcgccgccg
     2761 ccacgtcgca ggaggcaatg atcgcgtact gcgggctggt cgaggtatgc atcaggtaag
     2821 cctcgttgaa cacgtggcga tcgagtttcc gggtctgcga ctcctgcacc aggatctgcg
     2881 aggcctgcga tatgccggcc agcagcttgt gggtcgaatg cgtggcgaaa atcagcggat
     2941 ccttggggcg cggtcgatcc atgccgatcg cgtgcatgtt cttgtagaag ctgtggaacg
     3001 cggcatgcgg cagccaagcc tcgtcgaaat gcagtgtgtc gatcgatgcg ttcagcgtct
     3061 ccttgagcat ctcgacgttg tagaccaccc cgtcgtaggt actctgggtg atggtgagga
     3121 tgcgcggctt cttgcgcttg gccacgcgcg cgaacgggtt ggcctcgatt ttcttctgta
     3181 tggtttccgg cctgaactcg tcgagaggaa tcggcccgat gatgccgagg tgatttcttg
     3241 tcggcgtcag gaacacggga attgcgccgg tcatggtaat cgcgtgcagg atggacttgt
     3301 ggcagttgcg gtccaccacc acgatgtcgt ccggtgcgac gttggcgtgc cagaccatct
     3361 tgttcgaggt cgaggtgccg ttggtgacga agaagcaatg atcggcattg aagatgcgcg
     3421 cggcattgcg ctcggagtcg gcgaccggcc cgctatggtc gagcagttgc ccaagttcat
     3481 cgaccgcgtt gcagacatcg gcgcgcagca tgttctctcc gaagaactgg tggaacatct
     3541 gcccgaccgg actcttcagg aacgccacgc ctccggaatg cccggggcag tgccacgaat
     3601 acgagccgtc ctgggcgtaa tgcaccagtg cccggaaaaa cggcggcgcc aggctgtcca
     3661 ggtaggcgcg ggcctcgcgg atgatgtggc gcgcgacgaa ctccggcgtg tcctcgaaca
     3721 tgtggatgaa gccgtgcagc tcgcgcaaga tgtcgttggg aatgtgccgc gaggtgcgcg
     3781 tttcgccgta gaggtagatc gggatatcgg cattcctgaa gcgaagttcc ttgacgaagg
     3841 cgcgcaaggt tttcagcgcc tcggtggtct cctccacggt gccgccgccg aactcttcgt
     3901 cgtcgatcga gaggatgaag gccgaggcgc ggctttgctg ctgcgcgaag gaggaaaggt
     3961 cgccgtagct ggtgaccccg agcacttcga ggccttcgcc ttctatggcc ttggcgagcg
     4021 ctctcacacc gaggccgctg gcgttctccg agcggaaatc ctcgtcgatg atgacgactg
     4081 ggaagcgaaa tttcatgatg ttctccttgc cggaaccgtc ggtgccgggt ctcggcggga
     4141 gcggttgcgt ggatggcctg gcgtcgccac taaaggatgg gatctcgcgt cagattttcg
     4201 gaagagtcac gcctttttga ccctggtact tcccgccgcg gtcgcggtag gaggtctcgc
     4261 agacgtcgtc ggcatcggac tcgaagaaaa tgacctgggc gacgccctcg ttggcgtaga
     4321 tcttggcggg cagcggcgtg gtgttggaaa attccagtgt cacgtagccc tcccactccg
     4381 gctcgaaagg cgtgacgttg acgatgatgc cgcagcgcgc gtaggtcgac ttgcccaagc
     4441 aaatcgagag cacgttacgg gggatgcgga agtattcgac ggttcgggcg agcgcgaagg
     4501 aattcggcgg aatgatgcac acatccccct tgaaatcgac gaacgacttc tcgtcgaagt
     4561 tcttcggatc caccatcgtc gaattgatgt tggtgaaaat cttgaaattg ttcgagcagc
     4621 ggatgtcgta accgtagctc gaagtcccgt aggacacgat cttgtggccg ttggctacac
     4681 ggacctggcc cggctcgaac ggatcgatca tggcctgctc gcgcgccatg cggcgaatcc
     4741 acttgtccga ctt
//
LOCUS       DASQOP010000009         8464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1010198, whole genome shotgun sequence.
ACCESSION   DASQOP010000009 DASQOP010000000
VERSION     DASQOP010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8464
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010198"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="VE030_00280"
     CDS             <1..918
                     /locus_tag="VE030_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZF21880.1"
                     /translation="RTWQVLMSAEPSFRMRPDSIGELYVRSEKKGMVPIRALANVSYT
                     SGPDALDRFNNLPAVKLLGQGAPGVSSGQAIQVVERIADQVLPPDFGYDWGGASFQEK
                     KSSGTSSLALGLAAIMVFLILAAQYEKWSLPLSVMMALPFGTFGALAAVWLTKHTNDV
                     YFQIGLVTLLGLAAKNAILIVEYATLKHQQGLSPSAAALEAARLRFRPILMTSLAFIL
                     GVAPLAFSTGAGAGARTAVGTGVMGGMLAATFLAIFFVPMFFKLLTDRHLTEKRSTAQ
                     IRAEIEHHKTLAAQAAEVPHHAQTPKGEP"
     gene            918..2360
                     /locus_tag="VE030_00285"
     CDS             918..2360
                     /locus_tag="VE030_00285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZF21881.1"
                     /translation="MTPNRMVAATLAAVLLAGCMTIGPDYRRPRVDAPEQWPGTTTAE
                     PVSATWWKTYGDPVLDRMVEDALGYNTDLRFAIARVDEARAALGISRADQVPGVSANA
                     SASRNRASQESVLSIPPGIDPEYRDYRATLSASYEIDFWGKYRRATEAARAELLAARS
                     NREAVRLTLITDVARGYFNLRALDAQVLVTRRTIATRLASTALQRKRFEAGVASEFDL
                     RQVEAQIAQAQALLPAFERRVAEQETALAVLLGRSPRAIVTAPVERGAAIDALTLPPA
                     VPAGLPSDILERRPDVRQAEQQLIAANARIGQAKAAYYPSISLTAFLGGESAALSDLF
                     TTPARVWQIGGSAAQTIFDAGRTGQQVEQARAREQQLLTRYASAIQNAFKDTLDALVA
                     QRTARAALDAEQARVTALQNSYRLAQLRYDNGVSSLLDVLDVERGLLDSELNRIEAQR
                     AQLAATADLFKALGGGWDGVLEVEKSAKAN"
     gene            2657..3013
                     /locus_tag="VE030_00290"
     CDS             2657..3013
                     /locus_tag="VE030_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF21882.1"
                     /translation="MSIQFHSKNLSPVEISFLKWQYGFEEEDDPFERSLWHTILHAWE
                     ADRQAFMSNSPGRHLPRLGSDAAYPEQVALYVRFKSDQGESFWNDLIKRAGLADRRRD
                     NVVPIAERRRRTGSRG"
     gene            3055..4422
                     /locus_tag="VE030_00295"
     CDS             3055..4422
                     /locus_tag="VE030_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HZF21883.1"
                     /translation="MADTIKYLLSEEQIPKSWYNIMADLPSPPPPPLHPGTLKPLGPD
                     DLAPLFPMALIMQEVSAEREIEIPGPVREIYKQWRPSPLYRARRLEKVLGTPAKIYYK
                     YEGVSPAGSHKPNTAVAQAFYNRESGVRKIATETGAGQWGSALAFAGALFGIEVQVFM
                     VKVSYQQKPYRRALMETYGARCIASPSMETNVGRAILAKTPDSTGSLGIAISEAVEVA
                     ATHDDTKYALGSVLNHVLLHQTVVGQEAMKQMEMAGDDPDIIIGCTGGGSNFAGIVFP
                     FLGAQLRGGKRRRIVAIEPDACPSLTKGKYAYDFGDTGHLTPLVRMHTLGSTFIPPAV
                     HAGGLRYHGMAPLVSHIRQLGLIEARAYKQVEVFEAGVKFARAEGIVPAPEANHAVKG
                     AIDEALKCKAEGKARTILFNLCGHGHFDMQAYSDYFAGRLTDKSYDESELAMALAGLP
                     SVAAA"
     gene            4689..5210
                     /locus_tag="VE030_00300"
     CDS             4689..5210
                     /locus_tag="VE030_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HZF21884.1"
                     /translation="MKSSNDGFTLVELMIVVGIIGILASVALPAYENYAARAKISEAL
                     LALSTCKTSLTETTQSATTLPAAGQWGCESRAGAPAVSKYVTSVATSGEGAVRVVLQG
                     INPAADGQAIILRPWANDLRAAAVATGDVIGLWDCGPDPTNTQDISKLIPASCRATAA
                     QIGTVTAFAVSAS"
     gene            5337..6344
                     /locus_tag="VE030_00305"
     CDS             5337..6344
                     /locus_tag="VE030_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HZF21885.1"
                     /translation="MLIAKREIDSAGQRIRAQLAPTPLIRAQHFSRELGANVFFKLEM
                     LQPTHSFKVRGAFNAITQLSDKQRKRGVVTASGGNHGLAIAYAAATLGIPATVYLPET
                     ATDAKLAAIRRFGPEMVVHGSAWDDANALAMKVAGESGRAYIHPFDNPMVMAGQGTIL
                     TELLTQLPEPDLIVASIGGGGLISGILSAVQHYSSETRVFGVETEGADSMYQSRKAGK
                     IVELPAITSIAETLGARKTEKTQFEIVTQYVTDLVTVSDNAAIRALITILQEEKLLTE
                     PATSCSVAALLEGKIPVKPGENVVVVLCGANVAHEKVHEWESAARNLTVKARRRKSDP
                     K"
     gene            complement(6484..7518)
                     /locus_tag="VE030_00310"
     CDS             complement(6484..7518)
                     /locus_tag="VE030_00310"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HZF21886.1"
                     /translation="MRRELLRGMAAGVLATEATTVLMSQRVQSATLAQAALYVGEELA
                     RYGFPDGHPLGADRQGAFYREAAAQGLLEKVPVKAPRVAVREEIERFHSKRHVEKVMH
                     AERDRLEYLDNGDTPVFPGVYLASATVVGSALDGLERIMAGECVRTFQPIGGLHHARR
                     DHAAGFCVFNDLGVVIETLRSRYGIRRVAYVDIDVHHGDGIFYAFEDDPDLIFADIHE
                     DGNHLYPGTGREDETGTGAAKGTKLNIPLAPRSGDREFMQVWRRVAAHLTRFEPEFVV
                     FQCGADGLKGDPLAHLEYSPATHAHAAHSLVQIARRFSKGRLMAFGGGGYDRNNLAKA
                     WSAVLRAIAA"
     gene            complement(7529..7834)
                     /locus_tag="VE030_00315"
     CDS             complement(7529..7834)
                     /locus_tag="VE030_00315"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HZF21887.1"
                     /translation="MVRVYSAATLPDAHLVRGLLGQAGIDATVFNENLQGGLGEIPFT
                     HAYPEVWVVDERDLQRAREVIRQIELPSPGSGSVICPRCHEGSPGHFQVCWNCGEEF"
     gene            complement(7834..>8464)
                     /locus_tag="VE030_00320"
     CDS             complement(7834..>8464)
                     /locus_tag="VE030_00320"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZF21888.1"
                     /translation="SILEVLAACNHPCAIITKNAMVERDLDILAPMAAKNLVQVYISV
                     TTLDHDIARRMEPRASAPARRIEAIRRLAQAGVPAGVMVAPVVPFLTDSGIEALLEAA
                     REAGADRAGYTLMRLPWEVKDLFKDWLEHNFPLKAAHVMSRVRDMRDGRENDPNFGSR
                     MHGAGELAELLSKRFNMACRRLGLNHVRRGPLDTTKFKPPRLDGQMDLF"
BASE COUNT         1613 a         2728 c         2639 g         1484 t
ORIGIN      
        1 cgcacctggc aggtgctgat gtccgccgag ccatccttcc gaatgcgtcc cgattcgatc
       61 ggcgagctct acgtgcgttc ggaaaaaaag ggaatggtgc caatcagggc gcttgccaac
      121 gtcagctata cctccggtcc ggatgcactc gaccggttca acaacctgcc ggcggtcaag
      181 ttgctgggac agggcgcgcc gggcgtgtcc tcggggcagg ccatccaggt agtcgagcgc
      241 atcgccgacc aggtgctgcc gccggatttc ggctacgact ggggtggcgc ctcgttccag
      301 gaaaaaaaat cgagcggcac gtcctcgctc gcgctggggc tggcggcgat catggtgttc
      361 ctgattctgg cggcgcagta cgaaaagtgg tcgctgccgc tgtcggtaat gatggccctg
      421 ccattcggca cctttggcgc gctggccgcg gtatggctga ccaagcacac caacgacgtg
      481 tacttccaga tcggcctggt cacgctgctg ggccttgccg caaagaacgc gatcctgatc
      541 gtggaatacg ccacgctcaa gcatcagcag ggattgtcac cgtccgcggc ggccctggaa
      601 gccgcgcggc tgcgcttccg gccgatcctg atgacctcgc tcgcctttat cctgggcgtg
      661 gcgcctctcg ccttctccac gggagcgggt gccggagcac gcaccgctgt ggggactggc
      721 gtgatgggcg gcatgctggc cgcaaccttc ctcgcgattt tcttcgtgcc gatgttcttc
      781 aagcttctca ccgaccggca tttgaccgag aagcgctcga ccgcgcagat ccgggctgag
      841 atcgagcacc acaagacgct tgcggcgcag gctgcagaag tgcctcacca tgcgcagacg
      901 cccaaaggag aaccgtaatg acgcccaatc gcatggtcgc ggcgacgctc gccgccgtac
      961 tgctcgccgg ctgcatgaca atcggaccgg attaccgcag accgagagtg gatgcgccgg
     1021 agcaatggcc gggcacgacc actgcggaac ccgtctccgc cacctggtgg aagacctacg
     1081 gcgacccggt gctcgatcgc atggtcgagg acgcgcttgg ctacaacacc gatctgagat
     1141 tcgccatcgc gcgcgtggac gaagcccgtg ccgcgctcgg catcagccgc gcagaccagg
     1201 tcccgggcgt gtcggccaac gccagcgctt cgcgcaatcg cgccagccag gaaagcgtgc
     1261 tgagcattcc tcctggcatc gatccggaat accgcgatta ccgcgcgacc ctgagcgcct
     1321 cctacgaaat cgacttctgg gggaaatatc gccgtgccac cgaagcggcc cgcgccgaac
     1381 tgctggcggc acggtccaat cgcgaggcag tgaggctcac gctgatcacc gacgtcgccc
     1441 gcggttactt caacctgcgt gcgctcgatg cccaggtgct ggtgacccgg cgcacgatcg
     1501 ccacgcgact ggcttcgacc gcgctgcagc gcaagcgctt tgaagccgga gtggcatcgg
     1561 aattcgacct gcgccaggtc gaggcacaga tcgcgcaggc gcaggcgctg ctgcctgcgt
     1621 tcgagcgccg cgtggcagag caggagacag cgctggccgt cctgctcggt cgaagcccgc
     1681 gcgcgatcgt taccgcgccg gtggaacgcg gcgcggcgat cgatgcactc accttgccac
     1741 cggccgtacc cgcgggactg ccctcggaca tcctcgagcg ccgcccggac gtgcgccagg
     1801 cagagcagca gttgatcgcc gccaacgccc gcatcggcca ggcgaaggca gcctactatc
     1861 cgtcgatttc actgaccgcg ttcctgggtg gcgagagcgc tgcgctgtcg gatcttttca
     1921 ctaccccagc gcgcgtatgg cagatcggcg gttcggcggc acagacgatt ttcgacgccg
     1981 gccgcaccgg gcagcaggtc gagcaggcac gcgctcgcga acagcaattg ctgacccgct
     2041 acgcctccgc gatccagaac gcgttcaagg acaccctgga tgcgctggtt gcgcagcgca
     2101 ctgcacgcgc ggcgctggac gccgagcagg cgcgcgtcac tgccctgcaa aattcctaca
     2161 gactggcgca actgcgctac gacaacgggg tctcgagcct gctcgacgtg ctcgatgtcg
     2221 aacgcggact gctggattcg gaactgaacc ggatcgaagc gcagcgcgcg caactcgccg
     2281 ccaccgccga cttgttcaag gctctgggcg gaggctggga cggagtcctg gaagtggaga
     2341 aatccgccaa agcgaattaa gcccagggcg cgatgcgccc tgacaacagc gtttgacgca
     2401 aagaccgcaa aggaagaaga agaaaggcgc aaagaggaac acgggatcaa ggaaacggcg
     2461 atacgacagc gcgaccctcg aggcgacttg gccccggtct ttcagaccgc aggcgggggg
     2521 gtccgcgacc gccgctcccg cgcgagtcgc ctcccattta catgcggtac cggatgtcgt
     2581 ggcggttggc ttcggcgcaa actcccccta ttattgactg tcctagcctt cagcatccat
     2641 aagaggtcga ggagatatga gcatccagtt tcacagcaag aacctgtcac ctgtcgagat
     2701 ctccttcctg aagtggcagt acgggttcga ggaggaggac gacccgttcg agcgctcgtt
     2761 gtggcacacg atactgcacg cctgggaagc cgaccggcag gccttcatga gcaattcccc
     2821 cggccgccac ctcccgcgcc tgggttcgga cgcagcctat cccgagcagg ttgcgctata
     2881 cgtcaggttc aaatccgatc agggcgaatc gttctggaat gacctgatca agcgcgccgg
     2941 tttggccgac cgccgcaggg acaacgtggt gcccatcgcg gaacgccggc gcaggaccgg
     3001 atcaaggggc tgaaaatctg cccccgtcgg tccactaaat tggggggaga aatcatggcg
     3061 gataccatca aatacctgct ctccgaggag cagattccga agtcctggta caacatcatg
     3121 gcggacctgc cctcgccgcc tccgcccccg ctgcacccgg gtacgctcaa gccgctcggg
     3181 ccggacgatc tggcgccgct tttcccgatg gcgctgatca tgcaggaggt gtcggccgag
     3241 cgcgaaatcg agattcccgg cccggtgcgg gagatctata agcaatggcg gccgagtccg
     3301 ctttatcgcg cccggcgtct ggagaaggtg ctcggtaccc cggcgaagat ctattacaag
     3361 tacgagggcg tgagcccggc gggatcgcac aaaccgaaca cggcggtggc gcaggcgttc
     3421 tacaaccgcg agtcgggagt caggaagatc gccaccgaaa ccggcgccgg ccagtggggc
     3481 tctgcgctgg cgtttgccgg cgcgctgttc gggatcgaag tccaggtgtt catggtcaag
     3541 gtctcttacc agcagaagcc ttaccgccgc gcattgatgg agacctacgg cgcgcgctgc
     3601 atcgccagtc cctcgatgga aaccaacgtc ggccgcgcaa tcctggcgaa aacgccggac
     3661 agcaccggta gcctgggcat cgccatttcg gaagccgttg aagtcgccgc cacccacgac
     3721 gacaccaaat acgcgctggg ttcggtgctg aatcacgttc tgctgcacca gaccgtggtc
     3781 ggccaggagg cgatgaagca gatggaaatg gccggcgacg atcctgacat catcatcggc
     3841 tgcaccggcg gcggctcgaa tttcgcaggc atcgtatttc ccttcctggg tgcgcaactg
     3901 cgcggcggaa agcgacgccg catcgtcgcc atcgagccgg acgcatgccc ttcgctcacc
     3961 aagggtaagt acgcctacga tttcggcgac accggccacc tgacgccact ggtgaggatg
     4021 cacacgctgg gatcgacctt cattccgccc gcggtgcatg ccggagggct gcgctatcac
     4081 ggcatggcgc cgctggtttc gcatatcagg caactgggac tgatcgaggc gcgggcttac
     4141 aagcaagtcg aggtgttcga ggccggtgtg aagttcgcgc gcgccgaagg catcgttccg
     4201 gcgccggagg ccaaccacgc ggtcaagggc gcgatcgacg aagccctgaa gtgcaaggct
     4261 gaaggcaaag cgcgcaccat cctgttcaac ctgtgcggtc acggccactt cgacatgcag
     4321 gcctactccg actactttgc cggcaggctc accgacaaga gttatgacga gagcgaactg
     4381 gcgatggcgc tcgccggact gccttctgtc gctgctgcct agtttctaac ccgggttggc
     4441 gcttttcgtc atgtcttcca cgtggatttt ccgtcttgtc gtctggcgca gacgggcgcc
     4501 aagtccaggc gcccttcccg gttttcccca gctcgacact aactccatgt atttttatgt
     4561 attcatgtga tgcgtcgtcc cggacggacg cgcgaccatc ggtctggcat gtcgcttgcc
     4621 actctgggtc tgaatggcgt acggaaacgc gtgcgccacc caatacacct ccgactggag
     4681 ttgaaatcat gaagtcatcg aacgacggtt tcacgctcgt cgaattgatg atcgtggtgg
     4741 gaatcatcgg cattctcgcg tccgtcgcgc ttcccgccta tgagaattac gccgctcgcg
     4801 ccaagatttc ggaggccttg ctggctctaa gcacgtgcaa gaccagcttg acggaaacga
     4861 cccaatctgc gacgactctt cccgctgccg gccagtgggg atgcgaatca cgcgccggcg
     4921 cgcccgcagt gtcgaagtac gtaacgtccg tggcgacgag cggcgagggt gcggtgcgag
     4981 tggtgttgca ggggatcaat ccagccgccg acggccaggc gatcatactt cgtccctggg
     5041 cgaacgatct cagggcggca gccgtggcca caggcgatgt gatcggcctg tgggactgcg
     5101 gtccggatcc gacgaatacc caagacatat cgaagttgat cccagcctcc tgccgcgcaa
     5161 cggccgccca aatcggcact gtgactgcct tcgccgtgag tgcaagctaa gggagggaag
     5221 ccgtttgagc cccaagccat ctacaatgaa gtcagcggcc agccggtgac accggcctga
     5281 aatatccgga cctgagcggg cggcctagcg gccgcccgtt cttttgtgga gggaagatgc
     5341 tgatcgcaaa acgcgagatc gatagcgcgg ggcagcgcat ccgtgcgcag ctcgcgccga
     5401 ctcctttgat acgcgcgcaa catttcagcc gtgaactcgg ggccaacgta ttcttcaagc
     5461 tggagatgtt gcagccgacg cactcgttca aggttcgtgg tgcattcaat gcgattaccc
     5521 agctcagcga caaacagagg aaacgcggtg tcgtcaccgc ctccggcggc aatcacgggt
     5581 tggcgatcgc ctacgccgcc gccactctcg gcatcccagc gaccgtgtat ctgccggaaa
     5641 ccgccaccga tgcaaagctc gccgcgatac gccgcttcgg ccccgagatg gtcgttcatg
     5701 gcagcgcctg ggacgacgcc aacgcgctgg cgatgaaagt ggcgggcgaa tccggccgcg
     5761 cctatatcca tccgttcgac aatccgatgg tcatggccgg ccagggtacg atcttgaccg
     5821 aacttctgac gcaattgccg gagccggacc tgatcgtcgc ctcaatcggg ggtggtgggc
     5881 tgatttccgg aatcctctcc gcggtacagc attactcgtc tgaaacccgc gtattcggcg
     5941 tcgagaccga gggggcggac tcgatgtacc agagccggaa agcgggaaag atcgtggagc
     6001 tgccggcgat cacctcgatc gccgagaccc tgggcgcacg caagacggag aagacacagt
     6061 tcgaaatcgt cacgcaatac gttaccgatc tggtcacggt atcggacaac gcggccatcc
     6121 gggcgctgat taccatcctt caggaagaaa agctgctcac cgaaccggcg acgtcttgtt
     6181 cggtcgcggc gctgctggag ggcaagatcc cggtgaaacc cggtgagaac gtggtggtcg
     6241 tgttatgcgg ggcgaacgtg gcccacgaga aagttcacga atgggaatcc gcggcgcgca
     6301 atctcaccgt taaagcgcgc aggcgaaaaa gcgacccgaa gtaaagcaat tagatctttc
     6361 accgcccagg aagcgaagga cggtcaaagg cgaggcccga tacaaagggc gactactgaa
     6421 agcctgatcc ggcaggcatg gcatcccggt ttttctttgg gttctttgcg gtgaggtttt
     6481 gctctaggca gcaatcgccc gcagaaccgc actccaggct ttcgccagat tgtttcggtc
     6541 gtacccgccg ccgccaaaag ccatcagacg ccccttggaa aaacgcctgg cgatctgcac
     6601 caggctgtgc gccgcatgcg cgtgcgtggc cggcgaatac tcgaggtgcg ccagcggatc
     6661 gcctttcaat ccgtcggcac cgcactgaaa taccacgaat tccggttcga acctggtcag
     6721 gtgcgccgcc acccgccgcc agacctgcat gaactccctg tcgccagagc gcggtgcaag
     6781 cgggatgttg agcttggtgc ccttcgcggc tcccgtgccg gtttcgtcct cgcgcccggt
     6841 gccgggatag agatgattgc cgtcctcgtg gatgtcggca aagatcagat cgggatcgtc
     6901 ctcgaacgcg taaaagatac cgtcgccgtg atgcacgtcg atgtcgacgt aggccacgcg
     6961 cctgatgccg taccgggaac gcagcgtctc gatcaccacg ccgagatcgt tgaatacgca
     7021 gaatccggcc gcatgatcgc gccgggcatg atgaaggcct ccgatcggct gaaaggtgcg
     7081 cacgcactcg cccgccatga tgcgctcaag gccgtccaga gcggacccga ccacggtcgc
     7141 agacgcaagg tacacgccgg ggaacaccgg cgtgtcgcca ttgtcgagat actcgagccg
     7201 gtcgcgctcg gcgtgcatga ctttttcgac atggcgcttg ctgtgaaagc gctcgatttc
     7261 ctcacgcact gcgacccgtg gggctttaac cggcaccttc tccagcagtc cctgggcggc
     7321 ggcctccctg tagaacgcgc cctgccggtc ggccccgagc ggatgaccgt cggggaatcc
     7381 atagcgcgcc agttcctcgc cgacgtaaag cgccgcctgc gcgagcgtgg cactttgcac
     7441 tctttgcgac atcagcacgg tcgtagcttc cgttgcgagc acaccggccg ccattccccg
     7501 cagcagttcg cgccgcatcg catccggttc agaactcttc gccacaattc cagcagacct
     7561 ggaaatgtcc ggggctaccc tcatggcaac gcgggcagat cacggatccg gaacccggcg
     7621 acgggagctc gatctgacgg atcacttcgc gtgcccgttg caggtcacgc tcgtccacga
     7681 cccacacttc agggtaggcg tgggtgaaag gaatctcgcc cagcccgccc tgcaggttct
     7741 cgttgaatac cgtggcgtcg atccccgcct gtcccaacag accacgcacc aggtgcgcgt
     7801 ccggcaacgt ggcggcagaa tagaccctga ccatcagaac agatccatct ggccgtcgag
     7861 tctcgggggc ttgaacttcg tcgtatcgag cggcccgcga cgtacgtggt tcagcccaag
     7921 ccggcggcaa gccatgttga atcgcttgct cagcagctcc gccagttccc ccgcgccgtg
     7981 catgcgcgag ccaaagttcg gatcgttctc gcgcccgtcg cgcatgtcgc gtacgcgact
     8041 catcacgtgt gcggccttga gcgggaagtt gtgttcgagc caatccttga acaagtcctt
     8101 gacctcccag ggcagcctca tcagggtgta gccggcacgg tctgcgccgg cctcgcgtgc
     8161 cgcttcgagc agggcttcga ttcccgaatc ggtcagaaac ggcaccaccg gcgccaccat
     8221 tactccggcc ggcacgccgg cctgcgccag tctccggatc gcctcgatgc gccgcgccgg
     8281 tgccgaggcg cgcggttcca tgcgccgggc gatatcgtga tccagggtag tgactgaaat
     8341 atagacctga accagattct tcgctgccat cggcgccagg atatcgagat cgcgctccac
     8401 catggcgttc ttggtgatga tggcacaggg atgattgcag gctgccagca cttccagaat
     8461 cgag
//
LOCUS       DASQOP010000010        17926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2621877, whole genome shotgun sequence.
ACCESSION   DASQOP010000010 DASQOP010000000
VERSION     DASQOP010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 17926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17926
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2621877"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..446)
                     /gene="rplS"
                     /locus_tag="VE030_00325"
     CDS             complement(72..446)
                     /gene="rplS"
                     /locus_tag="VE030_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HZF21889.1"
                     /translation="MNLIKTLEQEEITRLGKNVPDFAPGDTVVVNVNVVEGDRKRVQA
                     YEGVVVAKRNRGLNSSFIVRKISSGEGVERTFQTYSPLIASIELKRRGDVRRAKLYYL
                     RDRSGKSARIREKLEGGPADPQ"
     gene            complement(443..1216)
                     /gene="trmD"
                     /locus_tag="VE030_00330"
     CDS             complement(443..1216)
                     /gene="trmD"
                     /locus_tag="VE030_00330"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HZF21890.1"
                     /translation="MIHLDVVTLFPEMFEAITGSGITQRARDRGFYQLVLWNPRDFST
                     NAYRSVDDRPYGGGPGMVMMAQPLSKAMASARQRQLSCGARRTRTIYLSPQGRPLTQK
                     IVEELGQLEGLVLLAGRYEGVDERLLQAEVQDQISIGDYVLSGGELPAMVLMDALIRR
                     IPGVLNDAESAQQDSFEEGLLDFPHYTRPELYQGEPVPAVLLSGHHVEIARWRMKQAL
                     GRTWERRPDLLAKLKLSADQAVLLEEYKAERNETQELIK"
     gene            complement(1227..1739)
                     /gene="rimM"
                     /locus_tag="VE030_00335"
     CDS             complement(1227..1739)
                     /gene="rimM"
                     /locus_tag="VE030_00335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HZF21891.1"
                     /translation="MSRPKPEPMVVMGRVSVPHGVKGWIKVQPFTQEIDGLLGYPHWW
                     LASDGEWWQHGIAESAVHGALVLARLEGCTDREAAVALKGAEVAIPRRLLPENREGEY
                     YWSDLLGMEVLNQRGERLGRVAKVLKTGANDVLVLEGEKERLVPFVGSVILNVDLADR
                     RLSVDWELDY"
     gene            complement(1720..1974)
                     /gene="rpsP"
                     /locus_tag="VE030_00340"
     CDS             complement(1720..1974)
                     /gene="rpsP"
                     /locus_tag="VE030_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003268924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HZF21892.1"
                     /translation="MVVIRLARGGARKRPFFNMVVADSRFARDGRFIERVGFYNPKAP
                     EGQESLRIQLDRVVFWQGKGAKLSDTAVRLVKQFSESAKT"
     gene            complement(2128..2895)
                     /locus_tag="VE030_00345"
     CDS             complement(2128..2895)
                     /locus_tag="VE030_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF21893.1"
                     /translation="MNRTLIAVVMLFSYCSAVLAMDYEREKRWSDQILPAILIGEPVW
                     LKQKNGHEFLALYTEAEKPRGAVIVGHGRGWNPDFELYGTLRVKLAEAGYTTLSIQLP
                     VLGPGAKVGDYLPTYADGAERYELAAKFLREKGYRSVAIVSHSLGATMANQYLIKADE
                     TTVKAWVFVGIINGLEEMFRIKIPVLDVFGSKDWEITQVGAYERKRQIDKVPGSQQVM
                     VPDALHFFEGKEDELVKVIVSFLDQVFHSGKPGEAGK"
     gene            complement(3017..4363)
                     /gene="ffh"
                     /locus_tag="VE030_00350"
     CDS             complement(3017..4363)
                     /gene="ffh"
                     /locus_tag="VE030_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HZF21894.1"
                     /translation="MLDSLTSRLAGVVKTLRGQARLTEANIREALREVRVALLEADVA
                     LPVVKEFIARVREKAVGEEVIGSLTPGQALVGVVQRELAELMGSRNDALNLAVVPPAV
                     ILLAGLQGSGKTTTAGKLAKLLGEQRKKTLLASCDVYRPAAIEQLATLARQLGVEMFA
                     SDPAQKPGEIAAGALDYARKHYHDVLIVDTAGRLAIDEAMMREIRQLHAVLDPVETLF
                     VVDSMQGQDAVNVAKAFADVLPLTGVVLTKIDGDARGGAALSVRQVTGRPIKFVGVSE
                     KPSGLEPFFPERLAARILGMGDVLALVEEARRNVDLEEAQNLARKVNAGKGFNLEDFK
                     NQMQQMKKLGGLSALIDKLPAKLAQAAQGAQMDERVIGRTVGIIESMTPQERAKPEVI
                     KASRKRRIAAGAGVSIQEVNRLLGQFEQMQKMMKMMGKGGLQKMMRGMKGGLPGMR"
     gene            4482..5303
                     /gene="ccsA"
                     /locus_tag="VE030_00355"
     CDS             4482..5303
                     /gene="ccsA"
                     /locus_tag="VE030_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HZF21895.1"
                     /translation="MGDILLHLLVSLGYAALAFFLWRDALLPSPPQGGQSRARWAHAA
                     LIAPLSLHAWLLARAAFGPEGLYLGVGNAVSVIIWLTVLIYWAGSFFYRLEGLQVLVV
                     PAAAVLSLLPLALPPVHPLANTQFAAFKAHLVIALLAYSLFTIASLHVLMMAMMERRL
                     HRGNLPRFLQGLPPLLTMEQLLFRIIAAGFVLLTLTLGSGILFSEALFGKPMAFTHKT
                     VFGILSWIIFAALLAGRAIYGWRGRVAMRWTLAGFLSLVLAYIGSKFVLEVLLQR"
     gene            5411..6682
                     /locus_tag="VE030_00360"
     CDS             5411..6682
                     /locus_tag="VE030_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HZF21896.1"
                     /translation="MDEIPLHTLLIAFVVLLVVSAFFSISETSMMALNRYRLRHLAKS
                     GHRGARLTTQLLARTDKLLGVILLGNNLVNAAAAGIATTIALRYFGQGEYAFLAATGI
                     VTFFILVFSEITPKVVGATYPERIAFPTAYVLSPLLRLVYPIVWFVNLFVQCLLWLLR
                     LQPKGNQPHTLSMEELRTLVLEGGNFLPQKHQNILLNLFDLESITVEDAMTPRVQIEA
                     IDIESSPDEIRRQIATSNHTRLPVYRGRVDDIIGIVHVRKVLNLSEGEQIGADTLKEI
                     MREPYFIPLDTPLLTQLQNFQEHQDRMALVVDEYGELKGLLTLEDILEEIIGEFTTHS
                     PMQTARFRRMDDGSIIVEGGSLLRELNRKLGYRFPLDGPKTLNGLILEHLQDIPEPGT
                     SLKLAGYPLEVLQTQDRVIRSVRLLPPEKDN"
     gene            6775..8496
                     /gene="pilB"
                     /locus_tag="VE030_00365"
     CDS             6775..8496
                     /gene="pilB"
                     /locus_tag="VE030_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HZF21897.1"
                     /translation="MVAATLEGTLSGLGRAMVQRGLLLERDAEAIQSQAKIAGIRFVE
                     QLILGKKLKESEIAEFAATTFGLPLLDIAAIDAEQMPLKAVDDKFLQSRRAVPVFQRG
                     NKLYVGLSDPTNRAVLDELKFQTGLQIEPVVVEDVKLGTLIQKLLKASDTSLKDLVGG
                     DVGADLLAEATPEEEPAPEAANDVDDAPIVRYLQKILLDAIGSGASDIHFEPYEKFYR
                     IRYRMDGILYEIAQPPLSLKEKIASRIKVISRLDIAEKRIPQDGRMKLTLSKNRAIDF
                     RVSILPTLHGEKIVMRILDPSSATLGIEALGYEPEQKDALLWAINRPYGMILVTGPTG
                     SGKTVSLYTCLNMLNEPGINIATAEDPAEINLAGINQVNVNDKAGLTFAAALKSFLRQ
                     DPDIIMVGEIRDLETAEIAVKAAQTGHLVLSTLHTNDAPTTLTRLLNMGVAPFNIASS
                     VILISAQRLARRLCKECKQPADIPGEALLRAGFKQADLDGSWKPYTAVGCDVCKGTGY
                     KGRVGIYQVMKISDELNRIIMTNGNAIDIAEQAQREGIRDLRQSGLLKVRQGMTSLEE
                     VEACTNE"
     gene            8516..9742
                     /locus_tag="VE030_00370"
     CDS             8516..9742
                     /locus_tag="VE030_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZF21898.1"
                     /translation="MATAAGAVRERIKEYTYLWEGKDRSGKAVKGEMRAGGEAMVNSV
                     LRRQGVTVLKIKRLKMGMGGKVSEKDITFFTRQLATMMKSGVPLLQAFDIVGKGHSNP
                     AVARLLMDIKNEVETGNSLNAAFRKYPLHFDALFCNLVGAGEQAGILDSLLDRLATYK
                     EKILAIKSKIKAALFYPVSIIVVAFIITAIIMIFVIPAFKDLFSNFGADLPAPTLLVM
                     AISDVFVKWWWAIFGGVGFGVWFFFYTWKRSVKMQIVMDRLTLKVPVFGPLVRKATIA
                     RWTRTLSTMFAAGVPLVEALDSVAGAAGNHVYYEATKKIQQDVSTGVSLTSAMTNVNV
                     FPNMVLQMVSIGEESGALDGMLSKVADFFEQEVDDAVEALSSLMEPMIMVVLGVLIGG
                     MVIAMYLPIFKMGQAV"
     gene            complement(9796..9924)
                     /locus_tag="VE030_00375"
     CDS             complement(9796..9924)
                     /locus_tag="VE030_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF21899.1"
                     /translation="MVLVYARRVVLEPSALALGPGVVHRVTGIADALRIGLFDGTC"
     gene            9949..10821
                     /locus_tag="VE030_00380"
     CDS             9949..10821
                     /locus_tag="VE030_00380"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HZF21900.1"
                     /translation="MSEPVIVTLVCTLLGLVVGSFLNVVIHRLPKMLEREWDAQAADL
                     LEQKGSADAVAKLRGTGSAARYNLVTPPSTCPRCGHRIRAWENIPILSYLFLRACCSA
                     CKAPISIRYPIVEALAGILSGYIGWRYGLTMATLGALFLAWSLIALTAIDIDTQLLPD
                     DITLPLIWLGLLVNLNGTFVPLQSAVIGAVAGYLSLWAVYWLFKIATGKEGMGYGDFK
                     LLAAIGAWLGWKMLPVVILLSSLVGAVVGIGLIVFARHGRNTPIPFGPYIAAAGLIAL
                     FWGETINRTYLQLF"
     gene            10824..11429
                     /gene="coaE"
                     /locus_tag="VE030_00385"
     CDS             10824..11429
                     /gene="coaE"
                     /locus_tag="VE030_00385"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434090.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HZF21901.1"
                     /translation="MPFAVGLTGGIGSGKSTVAELFAQLGAGVVDTDEIAHALTGPGQ
                     APVHEIARRFGPRYVTAAGALDRARMRKLAFEDARAKKDLESILHPLIRREAERRVAR
                     STAPYVIVVVPLLFESGAYKTLVRRVLVVDSDQEARIERVRRRNNLPREEILAIIASQ
                     VGQAEHLRRADDLIRNDGNVAALEPQVAALHRKYLDLAAES"
     gene            11541..12293
                     /gene="zapD"
                     /locus_tag="VE030_00390"
     CDS             11541..12293
                     /gene="zapD"
                     /locus_tag="VE030_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006756831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HZF21902.1"
                     /translation="MISYEFPLNERIRTLLRLEDLYGRAHYFASKSDPEEHHVALTSL
                     FEILDVGGRADLKSDLMQELERQKQHLDALRNNAAISQEALTSVLADIEKAISDMFLS
                     SGKTGQELRENDWLMSIKQRSAIPGGVCQFDLPSYHFWLNQNAEVRRLDLGEWLAPFL
                     PIRNGVQIILKLLRESGKTTTQLAHQGVYQQTMAGRLAQMLRIRLDSAYDCVPEISAN
                     KYALNVRFTTAIGMQRRTSVDDVKFELTFCNL"
     gene            12310..12510
                     /locus_tag="VE030_00395"
     CDS             12310..12510
                     /locus_tag="VE030_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HZF21903.1"
                     /translation="MKAEAKKTRMVNCPRCGTATPWSEANAYRPFCSERCKLIDLGQW
                     ATEGYRIPQEEADAGKSEGEER"
     gene            complement(12529..13452)
                     /locus_tag="VE030_00400"
     CDS             complement(12529..13452)
                     /locus_tag="VE030_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nudix family hydrolase"
                     /protein_id="HZF21904.1"
                     /translation="MVDVAAAVIERDDGSFLLAQRPHGKAYAGYWEFPGGKVEAGEPV
                     AEALRRELQEELGIDLVQAYPWITRVYSYPHASVRLHFHRVLKWLGEPHPHEEQALAW
                     QRCGALSVAPMLPANAPVLKALSLPIVYAITHAWETGVKRALAQLDDALARGLRLVQI
                     REGKLERPAREAFAAQVAQRVHASGGIVLVNGDERLAGRIGADGIHLPAQALMAARQR
                     SQFEWCGASCHDAAELARAAELGADFAVLGPVLEKPGQPDLPGIGLRCFGDLAGGRPL
                     PVFALGGMRLSDLDSARAHGAHGVAMIRGAW"
     gene            complement(13517..14404)
                     /locus_tag="VE030_00405"
     CDS             complement(13517..14404)
                     /locus_tag="VE030_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZF21905.1"
                     /translation="MSEFDRLIKRAEQVLERLEAALPPKPPAPEWKAAQAFRWRKRNG
                     RGFLQPVTHPHKIRLADLQRIDEQIRLVEQNTRQFLGGLTANDVLLTGARGTGKSSLV
                     KAVLNKFSAMGLRLIEVEKQDLMDLPDIVDLVADAPYKFILFCDDLSFDADEPGYKAL
                     KVILDGSISATSENLLIYATSNRRHLMPEYMQENLETKYLGEEIHPGESVEEKISLSE
                     RFGLWVSFYPFDQDEYLVIVDYWLGQLGVGERDRAKSRHEALQWALTRGSRSGRVAWH
                     FARDWAGRLRMKGEGGRRK"
     gene            complement(14408..15634)
                     /gene="argJ"
                     /locus_tag="VE030_00410"
     CDS             complement(14408..15634)
                     /gene="argJ"
                     /locus_tag="VE030_00410"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017333474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HZF21906.1"
                     /translation="MAVNLSAPDPAMLLPVKGVRIGIAEAGIRKANRKDLMLMTVSKG
                     TRVAGVFTRNRFCAAPVTVAKAHLQTGSIQALVVNTGCANAGTGEDGLARARQTCNEV
                     AALIGCTPEQVLPFSTGVIMEPLPVDRIAAGLPRCVSALAETNWAEAAQAIMTTDTVP
                     KAYSKRIDIGGHGVTLTGIAKGSGMIRPNMATMLGFVVTDVAVDQTLLQQATAYAAER
                     TFNCVTVDGDTSTNDAFILAATGRCGAPEIASSDSQEFKLFLDAVTEVAGFLARALVR
                     DGEGATKFITICVEGGMNETECRKVGYAIAHSPLVKTAFFASDPNLGRILAAIGYAGI
                     DDLDVAKVDLYLDDVMVAKGGSRATAYREEDGKRVMKQQEITVRVGLNRGASAATVWT
                     CDLSHGYVSINADYRS"
     gene            complement(15715..>17926)
                     /gene="secA"
                     /locus_tag="VE030_00415"
     CDS             complement(15715..>17926)
                     /gene="secA"
                     /locus_tag="VE030_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HZF21907.1"
                     /translation="YAADITYGTNSEFGFDYLRDNMVFQASERVQRGLTYAIVDEVDS
                     ILIDEARTPLIISGQADDSTDLYYRMNEIVPKLTRVKEENGPGDFTVDEKAHQVLLTE
                     TGHERAEDLLTKAGLLPENGSLYDAANIALMHHLYAGLRAHSLFHRDQHYVAQNDEII
                     IVDEFTGRLTPGRRWSDGLHQAIEAKEGVRIQRENQTLASITYQNYFRMYKKLAGMTG
                     TADTEAYEFQQIYGLETMIIPTHMPMIREDRMDQVYRTAREKYQAVINDIKDCYERGQ
                     PVLVGTTSIENSELLAAQLEKAKLPHQVLNAKQHAREAEIIAQAGRPKMVTIATNMAG
                     RGTDIVLGGNPEPEINKVSNDASLPDQEKEKRVAEMRDRWQKLHQQVVGAGGLHIIGT
                     ERHESRRVDNQLRGRSGRQGDPGSSRFYLALEDPLLRIFASDRVAAIMDRLKMPDGEA
                     IEHPWVTRAIENAQRKVEARNFDIRKQLLEYDDVSNDQRKVIYQQRNELLEATDISET
                     IRSMREGTVESLVSQHIPPDSVEEQWDVPGLERTLAGELGLELPLQQWLKQDESLDEE
                     SLRARIVAAANKHYEDKFSNVDAEAVHHYERAIMLDRLDSHWREHLAALDHLRQGIHL
                     RGYAQKNPKQEYKREAFDLFSLMLEAVRTEVSRILMTVKIRTAEEAQAVAEKEQVELK
                     NVQYQHADHDEALERANAETKVQPFVRGGQKVGRNDPCPCGSGKKFKQCHGKLG"
BASE COUNT         3272 a         5686 c         5395 g         3573 t
ORIGIN      
        1 aacaccaatg cggtcccgga aacgacaaag gcggccatag ccgcctttga ctttcgtagc
       61 tttggatatg cttactgcgg gtcggcagga ccgccttcca gtttttcgcg gatgcgcgcc
      121 gacttgccgg agcggtcgcg cagatagtaa agcttggcgc ggcgcacgtc gccgcggcgc
      181 ttcagctcga tgctggcgat cagcggcgaa taggtctgga aagtgcgctc cacgccttcg
      241 cccgaggaaa tcttgcgcac gatgaacgag gaattcaggc cgcggttgcg tttggccacc
      301 accacacctt cgtaggcctg cacgcgcttg cggtcgcctt cgaccacgtt tacgttgacc
      361 accacggtat cgcccggcgc aaagtcgggc acgttcttgc ccagccgggt gatttcttcc
      421 tgttcgagtg tcttgatgag gttcatttga tcaactcctg cgtttcgttg cgttccgcct
      481 tgtattcttc cagcagcacc gcctgatcgg cgctcaactt cagtttcgcc agcagatccg
      541 gccgccgctc ccaggtgcgc cccagcgcct gcttcatgcg ccaccttgct atctccacgt
      601 gatgccccga caacagcacc gcgggaaccg gctcgccctg gtacagctcc ggtcgcgtgt
      661 agtgcgggaa atcgagcaga ccctcttcga aagaatcctg ctgcgccgac tcggcatcgt
      721 tcaggacgcc cggaatgcgc cggatcagcg catccatcag caccatcgcc ggtagttccc
      781 cgccggaaag cacgtaatcc ccgatggaga tctggtcctg cacttcggct tgcagcaagc
      841 gctcgtccac gccttcgtag cgtcccgcca gcaacaccag gccttccagc tggcccagct
      901 cttccacgat cttttgcgtc agcggcctgc cctgcggcga cagataaatg gttcgcgtac
      961 gccttgcgcc acaactgagt tgccgctgcc ttgccgaagc catggctttc gacaacggct
     1021 gcgccatcat caccatgccg ggaccgccgc cgtaagggcg gtcgtcgaca ctgcgatagg
     1081 cgttggtact gaaatcgcgc ggattccaca gcaccagttg gtagaacccc ctgtcgcgcg
     1141 cccgctgggt gatgccgctg ccggtgatgg cctcgaacat ttcggggaac agagtgacga
     1201 cgtcaaggtg gatcattgcc gcacgctcag taatcgagtt cccagtccac cgacaggcgt
     1261 ctgtcggcca gatccacgtt gaggatcacg ctgccgacaa acgggaccag ccgttccttt
     1321 tcaccttcga gcaccagaac atcgttggcg ccggttttca gcaccttcgc cacgcgacca
     1381 agacgctcgc cgcgctggtt caacacttcc attccgagaa ggtcgctcca gtaatactcg
     1441 ccttccctgt tctcgggcag caggcggcgc ggtattgcaa cctcggcgcc cttcagcgcc
     1501 accgccgctt cgcggtcggt gcagccttca agtctggcga gcaccagcgc accgtgcacc
     1561 gccgattcgg caatcccgtg ctgccaccac tcgccatcgc tggccagcca ccaatgaggg
     1621 tagccaagca ggccgtcaat ttcctgggta aacggctgga ccttgatcca gcccttgact
     1681 ccgtgcggga cgctcacgcg acccattacc accatcggct caggttttgg ccgactcact
     1741 gaactgcttt accagacgca ccgccgtgtc cgacagcttg gcgcctttgc cctgccagaa
     1801 caccacacgg tccagctgga tgcgcaagga ctcctggccc tcgggcgcct tcggattgta
     1861 gaagccgacg cgctcgatga aacggccgtc gcgggcgaag cgcgagtcgg ccaccaccat
     1921 attgaagaac gggcgcttcc tggcaccccc gcgcgcaagt cgaatcacta ccatgacgtc
     1981 tatcctcaaa agcgaagcgc gcgattttac gccggttatt ccgtgatttg caagggaagt
     2041 ttgggcccgc gaagcggctc cgccaggccc ggtaaggggt tgcaagcgcc ggggcctgga
     2101 agggctccag ccgtcacgcc ccgagggcta tttcccggct tctccgggct ttccggaatg
     2161 gaacacctga tcgaggaaac tcacgatcac tttgaccagt tcgtcttcct tgccctcgaa
     2221 gaaatgcagt gcgtccggga ccatgacctg ctgcgaaccg ggtaccttgt cgatctgccg
     2281 tttgcgttcg taggcgccga cctgggtgat ctcccagtcc ttgctgccaa acacgtccaa
     2341 caccgggatc ttgatgcgga acatttcttc caggccgttg atgatgccga cgaacaccca
     2401 ggccttgacc gtggtttcgt cggccttgat cagatactgg ttggccatgg tcgcgcccag
     2461 gctgtgcgaa acgatggcaa cgctgcggta gcccttctcg cgcaggaatt tggcggccaa
     2521 ttcgtagcgt tccgccccgt cagcataggt gggaaggtaa tcgccgacct ttgcgcccgg
     2581 cccaagcacc ggcaactgga tcgaaagcgt ggtgtagccg gcctccgcaa gcttgacgcg
     2641 caaggtgccg tagagctcga agtcggggtt ccagccgcga ccgtgaccga cgatgacggc
     2701 accgcgcggc ttctcggcct cggtgtacag cgccaggaat tcgtgaccgt ttttttgctt
     2761 cagccagacc ggctcgccga tcaggatcgc gggcagaatc tggtccgacc agcgtttctc
     2821 gcgctcgtag tccatcgcca ggacggcgga acagtacgag aacagcatga caacggcaat
     2881 caacgttcga ttcatcggga gcggctttct ttggcggggg ccggcattgt accggagggc
     2941 atgtgtgagc taagaccagc gggaatcaga gggggttccg gcaccgacga taagcgtcgg
     3001 aaacgcaggc gaggcgctag cgcatcccgg gcaacccgcc cttcatcccc cgcatcatct
     3061 tctgcagccc gcccttgccc atcattttca tcattttctg catctgctcg aactggccga
     3121 gcaggcggtt gacctcctga atcgacacgc cggcgccggc ggcgatgcgg cgcttgcggg
     3181 aggccttgat gacctcgggt ttggcacgtt cctgaggcgt catcgattcg atgataccca
     3241 cagtgcggcc gatcacacgc tcgtccatct gcgcgccctg tgcggcttgc gcgagtttcg
     3301 ctggcaactt gtcgatcagc gccgacaagc cgcccagctt cttcatctgc tgcatctggt
     3361 tcttgaaatc ctcgagattg aagcccttgc cggcgttcac tttcctggca aggttctggg
     3421 cttcctcgag gtcgacgttt ctgcgcgcct cctccaccag cgccagcacg tcgcccatgc
     3481 cgaggatgcg tgccgccagg cgttcgggaa agaacggctc cagtccgctc ggcttctccg
     3541 acacgccgac gaacttgatc ggcctgccgg tcacctggcg gaccgaaagc gcggcaccgc
     3601 cgcgagcgtc cccgtcgatc ttggtgagca ccacgccggt gagcggcagc acatcggcaa
     3661 aggccttggc cacgttcacc gcgtcctggc cctgcatcga gtccaccacg aacagcgttt
     3721 ccaccggatc gagcacggcg tgaagctggc ggatctcccg catcatggct tcgtcgatcg
     3781 ccagtcggcc ggcggtgtcc acgatcaaca cgtcgtgata atgcttgcgc gcgtagtcga
     3841 gcgccccggc ggcaatctcc ccgggttttt gcgcagggtc ggacgcaaac atttccacgc
     3901 cgagctgacg ggccagggtt gcaagctgtt cgattgcggc gggtctatag acgtcgcaac
     3961 tcgccagcag tgttttcttc ctctgttcgc cgagcagctt cgccagtttg ccggcggtcg
     4021 tggtcttgcc cgatccctgc aggccggcca gcaggatcac cgcaggagga acaaccgcca
     4081 ggttgagagc gtcgttgcgg ctgcccatca gctcggccaa ctcgcgctgc accacgccca
     4141 ccagcgcctg cccgggcgtg aggctgccga tcacttcctc gcctaccgcc ttttctctga
     4201 cccgcgcgat gaattctttc actaccggga gcgcgacgtc ggcctcgagc agcgcgacgc
     4261 gtacttcgcg caacgcctcc cggatgttgg cttcggtcag gcgcgcctgg ccacgcagcg
     4321 tcttgacgac gccagcgagc cggctggtga ggctgtcgag catccgctgt ggtcctggaa
     4381 tgtggagcag atggggataa tgtagactgc gtaagccaag tccacgtatc tgctagtgcc
     4441 ctgttccgat gattcgtggg ataactcgtg aaccttgcta gatgggcgac attttacttc
     4501 atcttctggt gagtctcggc tacgccgcgc tggccttctt cctctggcgc gatgcgctgc
     4561 tgccgtcgcc gccgcaaggc ggacagtcga gggcgcgctg ggcgcatgcg gcgctgattg
     4621 cgccgttgtc gctgcatgcg tggctgctgg cgcgcgccgc gtttgggccg gagggccttt
     4681 acctcggtgt cggcaacgcg gtttcagtga tcatctggct gacggtgctg atctactggg
     4741 ccggcagctt tttctaccgg ctcgaaggcc tgcaggtgct ggtcgtgcct gcggcggcgg
     4801 tactgtccct gttgccgctc gcgttgccgc cggtgcatcc gcttgcgaac acccagttcg
     4861 ccgcgttcaa ggcgcacctg gtcatcgcat tgctggctta cagcctgttc accatcgcgt
     4921 cgctgcacgt gctgatgatg gcgatgatgg agcgccgcct gcaccgcgga aacctcccgc
     4981 gattcctgca gggccttccg ccactgctga ccatggagca gttgctgttc cgcatcatcg
     5041 ccgccggctt cgtgttgctc acgctgacgc tcggcagcgg catcctgttt tcggaagcct
     5101 tgttcggcaa gccgatggca ttcacccaca agacggtgtt cggcatcctc tcctggatca
     5161 tcttcgcggc gctgctcgcc gggcgcgcaa tctacggctg gcgcgggcgc gtggcgatgc
     5221 gctggacgct ggccggtttc ctgtcgctgg tgctcgccta catcggcagc aaattcgtgc
     5281 tcgaagtgct gttgcaacgc tgaacggcgg ccgcgcagca accgggggta atcgcagcgg
     5341 cgactgcctt gacactgttt gtcgcgctgc cgatactgat ttcgacttta cgacccggtg
     5401 gtgaccgcac ttggacgaaa ttccgctgca tacgctgctg atcgcatttg tggtcctgct
     5461 cgtcgtttcc gctttttttt ccatttccga aaccagcatg atggcgctaa accgctaccg
     5521 gttgcggcat ctggccaaga gcgggcatcg cggcgcccgc ttgaccactc aattgctggc
     5581 aaggacagac aagctgctcg gcgtcattct gctcggaaac aatctggtga acgccgccgc
     5641 cgccggcatc gccaccacga tcgcattgcg ttacttcggc cagggcgagt atgcatttct
     5701 cgccgccacc ggcatagtta cgttcttcat cctggtattc agcgagatca cccccaaggt
     5761 ggtcggggcg acatacccgg agcgcatcgc ctttccgacg gcctatgtgc tctcaccgct
     5821 gctcaggctg gtttatccga tcgtctggtt cgtcaatctt tttgtccagt gcctgttgtg
     5881 gctcctgcgc ctgcagccga agggcaacca gccgcatacg ctgagcatgg aagaattgcg
     5941 caccctggtg ctggaaggtg gcaactttct gccgcaaaaa caccagaaca tcctcctcaa
     6001 cctgttcgac ctcgagtcga tcaccgtgga ggacgcgatg actccacgcg tccagattga
     6061 ggccatcgac atcgaatcgt cccccgacga gatccgccgc cagatcgcca ccagcaatca
     6121 cacgcgcttg ccggtctacc gcgggcgggt ggacgacatc atcggcatcg ttcatgtgcg
     6181 caaggtgctc aacctttccg agggcgaaca gatcggcgca gacactctca aggagatcat
     6241 gcgcgaaccg tacttcattc ccctggatac gccgctgctc acccagttgc agaatttcca
     6301 ggagcatcag gacaggatgg cgctggtggt ggacgaatac ggagaactga agggtctgct
     6361 cacactcgag gacatccttg aggagatcat cggcgaattc acgacgcatt ctccgatgca
     6421 aaccgcgcgc tttcgccgga tggacgacgg cagcatcatc gtggagggcg gcagcctgtt
     6481 gcgggaactg aaccgcaagc tcggctaccg cttccccctc gacggcccca agaccctgaa
     6541 tggcttgatc ctcgagcacc tgcaggacat cccggagccg ggcaccagcc tcaaactcgc
     6601 aggctacccg ctggaggtgc tccagaccca agaccgggta atccgctcgg tgcgcctgct
     6661 gccacccgaa aaggacaatt aatcaggacc attaatcaag gacttccgtc tttactttaa
     6721 ggagcgctgg gtgcatcatt tgactgtttt ccaccgctga ctatagagat agagatggtt
     6781 gcagcaacgc tagaaggcac gctgtccggc ctcggccgtg cgatggtgca gcggggcctc
     6841 ctgctcgagc gcgacgcgga ggcaatccag tcccaggcaa aaatcgcggg gatcagattc
     6901 gtcgaacagc tcatcctcgg caagaagctc aaggagagtg agatcgccga atttgcggcg
     6961 accactttcg gcctgcctct gctcgacatt gccgccatcg acgccgagca gatgccgctc
     7021 aaggcagtgg atgacaaatt cctgcaaagc cgccgcgcgg tgccggtgtt tcagcgcggc
     7081 aacaagctct atgtcgggct gtcggacccg accaaccgcg ccgtcctcga cgagctgaaa
     7141 ttccagaccg gcctgcagat cgagccggtc gtcgtcgagg acgtcaagct cggcacgctg
     7201 atacagaagc ttctcaaggc cagcgatacc tcgctgaagg atctcgtggg cggggatgtg
     7261 ggtgccgacc tgctcgccga ggccacgccc gaggaggagc cggcgcccga ggccgcaaac
     7321 gatgtcgatg acgcaccgat cgtgcgttat ctccagaaaa ttctgctcga cgcaatcggc
     7381 agcggcgctt ccgacatcca cttcgagccc tacgaaaagt tctaccgcat ccgttaccgg
     7441 atggacggca tactctacga aatcgcccaa ccgccgctgt ctctgaagga aaagatcgcc
     7501 tcccggatca aggtcatttc gcgtctggat atcgccgaaa agcgcattcc gcaggacggc
     7561 cggatgaaat tgaccctgtc caagaatcgc gccatcgact ttcgtgtcag catcctgccg
     7621 acgctgcatg gggaaaagat cgtgatgcgc attctcgatc cgtcgagcgc gacgctgggc
     7681 atcgaggcac tgggctacga gccggaacag aaggacgcgc tgctgtgggc catcaaccgg
     7741 ccctacggaa tgatcctggt gaccggtccg accggttcag gcaagacggt atcgctgtac
     7801 acctgcctga acatgctgaa cgagcccggc atcaacattg ccaccgccga ggatccagcg
     7861 gaaatcaacc tggcggggat caaccaggtc aacgtcaacg acaaggcggg gctgactttt
     7921 gccgcggcgt tgaagtcgtt cctgcgccag gacccggaca tcatcatggt cggcgaaatc
     7981 cgcgatctgg aaacggcgga aatcgcggtg aaagcggcgc aaaccggcca cctggtgctg
     8041 tcgactctgc acaccaacga cgcaccgacc acgctgacgc gactgttgaa catgggagtg
     8101 gctcctttca acatcgccag ttcggtcatc ctgatctctg cgcagcgctt ggcgcgtcgg
     8161 ctgtgcaagg agtgcaagca gcccgccgac attcccgggg aggcgctact gcgggcgggg
     8221 ttcaagcaag ccgatcttga cggcagttgg aaaccgtaca cggcggtcgg gtgcgacgtt
     8281 tgcaagggca ccggctacaa gggccgggtc ggcatttacc aggtcatgaa gatcagcgac
     8341 gaattgaacc gcatcatcat gaccaacggc aacgccatcg acattgccga gcaggcgcag
     8401 cgtgagggta tccgcgacct gcggcaatcg ggattgctga aggtacggca gggcatgact
     8461 tcactcgaag aagtcgaagc ctgcaccaat gaataacact tgacgaggta acgctatggc
     8521 tacggcagca ggggcagtac gcgaaaggat caaggaatac acctacctct gggaaggtaa
     8581 ggacagatcc ggcaaggcag taaagggcga gatgcgcgcc ggcggcgaag cgatggtcaa
     8641 ttcggtactg cgccgccagg gcgtcacggt cctcaagatc aagcggctga agatggggat
     8701 gggcgggaag gtcagcgaaa aggacatcac gttcttcacc cgccagctgg cgacgatgat
     8761 gaagtcgggc gtgcctttgc tccaggcctt cgatatcgtc ggcaaggggc actccaatcc
     8821 cgcggtggcc aggctgctga tggacatcaa gaatgaagtg gaaaccggca acagcctgaa
     8881 cgccgctttc cgcaagtatc cgctgcattt cgacgcgctg ttctgcaacc tggtgggcgc
     8941 cggggagcag gccggtattc tcgacagcct gctggaccgt ctggcgactt acaaggaaaa
     9001 gatcctcgcg atcaaaagca agatcaaggc cgcgctgttc tatcccgtat cgatcatcgt
     9061 ggtggcgttc atcatcactg cgatcatcat gattttcgtg atcccggcgt tcaaggatct
     9121 gttctcaaac ttcggcgccg acctccccgc gcccacactg ttggtgatgg cgatttcgga
     9181 tgtcttcgta aaatggtggt gggcaatttt cggcggcgtg ggcttcggtg tatggttctt
     9241 cttctacact tggaagcgct cggtaaagat gcagatagtg atggaccggc tgaccctcaa
     9301 ggtgccggtt ttcggcccgc tggtccgcaa ggcgactatt gcgcgctgga cccgcacgtt
     9361 gtccaccatg tttgcagccg gcgtgccgct cgtggaggcg ctcgattcgg tggcgggcgc
     9421 cgcaggcaac cacgtgtatt acgaagccac caagaaaatc cagcaggacg tcagtaccgg
     9481 cgtgagcctc accagcgcca tgactaatgt caacgtcttt ccgaacatgg ttctgcaaat
     9541 ggtgtcgatc ggcgaggaat cgggcgcctt ggacgggatg ctcagcaagg tggccgactt
     9601 ttttgagcag gaagtggacg atgcggtcga agccttgtcc agcctgatgg aaccgatgat
     9661 catggtggtt ctgggggtgc tgatcggcgg catggtaatc gccatgtacc tgccgatctt
     9721 caaaatgggc caggccgtat aagcggcgat cgcttcgatt aatcatgcgc ccgacgagct
     9781 cgggcgtttt ttttatcagc acgtgccgtc gaacagcccg atgcgcaacg cgtctgcgat
     9841 gccggtgact ctatgcacaa ctccaggccc cagtgccagc gcgctcggtt ccagcacgac
     9901 acgacgcgca tacacaagca ccactgaaat atgaagctca cagacctcat gagtgaaccg
     9961 gtaatcgtga cgctggtctg caccctgctc ggactggtgg tcgggagttt tctaaacgtg
    10021 gtcatccatc ggctgccaaa aatgctcgag cgcgaatggg acgcgcaggc agccgatctt
    10081 ctggagcaga agggctctgc cgatgccgtg gcaaagctga ggggcacagg cagcgccgcg
    10141 cggtacaacc tggtcacacc gccctcgacc tgcccgcgct gcggccaccg catccgcgcc
    10201 tgggaaaaca ttccgatcct gagttatctc ttcctgcgtg catgttgctc ggcgtgcaag
    10261 gcgccgatca gcatccgcta cccgatcgta gaggcgctgg ccggcatcct ctccggctac
    10321 atcggctggc gctacggact gaccatggcg acgctgggcg ccctttttct ggcgtggtct
    10381 ctgatcgcgc tgaccgccat cgatattgat acgcagttgc tgccggacga cattactctg
    10441 ccgctgatct ggctcggact gctggtgaac ctgaacggca ccttcgtgcc actgcagagt
    10501 gcggtcatcg gggccgtcgc cggctacctg tcgctgtggg ccgtgtactg gctgttcaag
    10561 atcgccaccg gcaaggaggg aatgggctac ggcgacttca agctgctcgc cgcgatcggt
    10621 gcgtggctgg gttggaaaat gttgccggtg gtaatcctgc tctcctccct ggtgggcgcg
    10681 gtcgtcggca tcggcctgat agttttcgcc cgccatggcc gtaacacgcc gattccgttc
    10741 gggccctaca ttgctgcggc gggattgatc gcgctgttct ggggcgagac gatcaaccgg
    10801 acttacctgc agctgttctg acgatgcctt ttgccgtggg tctcaccgga gggatcggct
    10861 cgggcaagag cacggttgcc gaactgttcg cgcaactcgg tgccggcgtg gtggacactg
    10921 acgagattgc gcacgcgctg acaggaccgg gccaagcgcc ggttcacgag atagcacggc
    10981 gctttggtcc gcggtacgta accgcagccg gcgccctgga ccgcgcccgg atgcgcaaac
    11041 tggcattcga ggatgcgcgc gcgaagaaag acctggaatc catcctgcat ccgctgatcc
    11101 ggcgcgaagc cgagcgccgg gtcgcgcgat cgactgcacc ctacgtcatt gtggtggtgc
    11161 cgctgctgtt cgaatccggc gcttacaaga ccctggtgcg gcgcgtgttg gttgtggaca
    11221 gcgatcagga agcacggatc gagcgcgtca ggcgacgcaa caacctgccg cgcgaagaaa
    11281 ttctggcgat catcgcctcc caagtgggac aggccgagca cctgcgccgc gcggatgact
    11341 tgatcagaaa cgacggcaac gttgcagccc tggagccgca agtcgccgct ttgcatcgca
    11401 aatacctgga tctcgccgca gaaagctagc gacggcgctg caagacggca tcgccggctt
    11461 gcagctttgc cgcattcagc tttattttta cgggcttttc gggaatactt gcccttactc
    11521 tggtaagctg cgcccgcatt gtgatcagct acgagttccc gctaaacgag aggattcgca
    11581 ccctgctgcg cttggaggat ctgtacgggc gcgcgcacta tttcgcctca aaaagcgatc
    11641 cggaggaaca tcacgtcgct ctgacgtcgc tgttcgagat cctcgacgtc ggaggccgcg
    11701 ccgatctcaa gtccgacctg atgcaggaac tggaacgcca gaaacaacac ctagacgcat
    11761 tgcgcaacaa tgccgcgatc tcgcaggaag ccctgacatc cgtgcttgcc gatatcgaga
    11821 aagcgatttc cgacatgttt ctctcatcgg ggaaaaccgg tcaggagttg cgcgagaacg
    11881 actggttgat gagcatcaag cagcgttccg cgatacccgg cggcgtgtgc cagttcgatc
    11941 tgccttcgta tcacttctgg ctcaatcaaa acgctgaagt gcgccgtctc gatctgggcg
    12001 aatggcttgc gcctttcctg ccgatccgca acggcgtgca gatcatcctg aaattgctgc
    12061 gggagagcgg caagaccacc acccagctcg cgcatcaggg ggtgtaccag cagaccatgg
    12121 cgggccggct ggcgcagatg ctgcgcatcc gcctggacag cgcatacgac tgcgtaccgg
    12181 aaattagcgc caacaaatac gcgctcaacg tgcgcttcac caccgccatc ggcatgcagc
    12241 gtcgtaccag cgtcgacgac gtgaaattcg aactgacgtt ctgcaacctg tgatgaatgc
    12301 ctggctgcga tgaaagccga agcaaagaaa acgcgcatgg tgaattgccc gcggtgcggg
    12361 accgccacgc cgtggagcga ggccaacgcc taccggccgt tctgttccga acgctgcaag
    12421 ctgatcgacc tgggccagtg ggccaccgag ggctaccgga ttccacagga ggaagcagac
    12481 gcgggcaagt ctgaaggcga agagcgttag ccgcggcgag cgtagcgcct accaggcgcc
    12541 gcggatcatg gccaccccgt gcgcgccgtg tgcacgtgcg ctgtcgagat ccgacaatcg
    12601 catcccgccg agtgcaaaca ccggcagcgg gcgcccgcct gcgagatcgc cgaagcaccg
    12661 caggccgatc ccgggcaggt ccggctgacc gggcttttcc aggaccggcc caagcaccgc
    12721 gaaatcggcg ccgagttccg ctgcgcgcgc cagttcagcc gcatcgtgac aggaggcgcc
    12781 gcaccattcg aattgcgacc gctgtcgagc cgccatcaac gcttgcgcag gcaggtgtat
    12841 gccatcggca ccgatccgtc cagccagtcg ttcgtcgcca tttaccagga caatgccccc
    12901 cgaggcatgc acgcgctgcg cgacttgcgc agcgaaagcc tcgcgcgccg gtcgttcgag
    12961 ttttccttct ctgatctgca ctagacgcag acccctcgcg agcgcgtcgt cgagctgcgc
    13021 gagcgcgcgc ttgacccctg tttcccaggc atgcgtgatg gcgtaaacga tgggcagcga
    13081 cagcgctttc aggaccggcg cgttggccgg aagcatcggc gcgaccgaca atgcgccgca
    13141 gcgctgccaa gccagcgctt gctcttcgtg cggatgcggt tcgcccagcc atttcagcac
    13201 gcgatgaaag tgcaggcgca cgctcgcgtg cgggtagctg taaacgcggg taatccaagg
    13261 ataggcctgc acgaggtcga ttcccagctc ctcttggagt tcgcgcctta gcgcctcggc
    13321 gacaggctcg ccggcttcga ccttgccgcc ggggaactcc cagtagccgg cataggcctt
    13381 tccatgcggc ctctgcgcaa ggagaaagct gccgtcgtcg cgctcgatca ccgcagccgc
    13441 aacgtccacc accttcgaag gatgaaagtt gaaggatgaa ggatgaaggc aatccccagc
    13501 cttgggattg ggttgcttac ttccgccttc cgccttcacc cttcatcctt agtcttcccg
    13561 cccagtcgcg ggcgaaatgc cacgccacgc gaccgctgcg cgaaccgcgg gtaagcgccc
    13621 attgcaaggc ctcgtgacgg gacttcgcgc gatcgcgttc acccacgccg agctgcccga
    13681 gccagtagtc cacgatcacc aagtactcat cctgatcgaa aggatagaag gaaacccaca
    13741 gaccgaaacg ctcggacaga gaaatttttt cctcgacgct ctcgccggga tggatttcct
    13801 cgccaagata cttggtctcg aggttttcct gcatgtactc aggcatcagg tggcgtcggt
    13861 tcgaagtggc gtatatcagc agattttccg aagtcgccga gatcgagccg tcaaggatga
    13921 ctttcagcgc cttgtaaccc ggttcgtcgg cgtcaaagga cagatcgtcg cagaacagga
    13981 tgaatttgta cggcgcatcg gcaaccaggt cgacgatgtc cggcagatcc atcagatcct
    14041 gtttctccac ctcgatcagg cgcaagccca ttgccgagaa tttgttgaga acggccttta
    14101 ccagcgagga cttgccggtg ccgcgcgcgc ctgtgagcag cacgtcgttc gcggtcagcc
    14161 ctccgaggaa ctgccgggtg ttctgttcga ccagccggat ctgctcgtcg atccgctgca
    14221 ggtcggcgag gcggatcttg tgcgggtgcg tcaccggctg caggaagcca cgaccgttgc
    14281 gcttgcgcca gcggaacgcc tgtgccgcct tccattccgg tgccggcggt ttgggcggca
    14341 gggcagcctc aagccgctcc agcacctgtt cggcgcgctt gatcagtcgg tcgaattcac
    14401 tcatcgctca actgcggtag tcggcgttga tgctcacgta gccgtgcgac agatcgcagg
    14461 tccacaccgt ggccgccgaa gccccgcggt tgagtcccac acgcaccgtg atctcctgct
    14521 gcttcatcac ccgcttgcca tcttcttcgc ggtaggccgt tgcacggctg ccgcctttcg
    14581 ccaccatcac atcgtcgagg tacagatcga ctttggcaac gtcgagatcg tcgatgcctg
    14641 cgtagcctat cgccgcgagt atgcggccga gattcggatc tgaagcgaag aacgccgtct
    14701 tcaccagcgg cgaatgggca atggcgtagc cgaccttgcg gcattcggtc tcgttcattc
    14761 cgccttcgac gcagatggtg atgaacttgg ttgctccttc gccatcgcgc accagcgccc
    14821 gcgccaggaa gcctgcgact tcggttaccg catccaggaa cagcttgaat tcctgcgaat
    14881 cggagctggc gatctcgggc gcgccgcacc ggccggtagc cgccagaatg aaagcatcgt
    14941 tggtggaagt gtcgccgtcg acggtcacgc agttgaacgt gcgttccgca gcgtaggcgg
    15001 tcgcctgctg cagcagcgtc tggtcaaccg ccacatccgt aaccacgaag cccaacatag
    15061 tcgccatgtt cggccggatc attcccgaac ccttggcaat acccgttaag gtcacgccat
    15121 ggccgccgat gtcgatgcgc ttcgagtacg ccttcggcac ggtgtcggtg gtcatgatcg
    15181 cctgcgccgc ctccgcccag tttgtctccg cgagcgcact gacgcagcgc ggcagcccgg
    15241 cggcgatccg atcgaccgga agcggctcca tgatgacgcc ggtggaaaac ggtaacacct
    15301 gttccggtgt gcaaccgatc agcgcggcga cctcgttgca ggtctgcctg gcgcgcgcca
    15361 gcccgtcctc accggtgccg gcgttggcgc agccggtatt gacgacaaga gcctgaatcg
    15421 agccggtctg aagatgcgcc ttcgccaccg tgactggtgc ggcgcagaag cggttgcggg
    15481 tgaacacacc ggcaacccgc gtccccttgg acacggtcat cagcatcaga tccttgcgat
    15541 tggctttgcg gatgccggct tcggcgatgc cgatgcgcac gcctttcacc ggcagcagca
    15601 ttgcaggatc aggagcagat agatttaccg ccatttgccg gctccggccc gtccgctctc
    15661 gctatttgcc ggcgcgccgt gcacggtagc tcgaccctcg cgcatgcgct tcgtttagcc
    15721 gagcttaccg tggcactgct tgaacttctt accggacccg cacgggcagg ggtcgttgcg
    15781 tccgaccttt tggccaccgc gcacgaacgg ctgaactttg gtttcggcgt ttgccctttc
    15841 cagcgcctcg tcgtgatcgg catgctggta ctgcacgttc ttgagttcga cctgttcctt
    15901 ctcggcgacg gcttgcgcct cctcggcagt cctgatcttc acggtcatca ggatgcggct
    15961 gacctcggtg cgcacggctt cgagcatcag cgaaaacaga tcgaaggcct cgcgcttgta
    16021 ctcctgcttc ggattcttct gcgcataacc ccgcagatgg ataccctggc gcaggtgatc
    16081 tagtgcggcg aggtgctcgc gccagtggct gtccagccgg tccagcatga tcgcgcgctc
    16141 gtagtgatgc acggcttccg catccacatt gctgaacttg tcctcgtaat gcttgttggc
    16201 ggccgcgacg atgcgggcgc gcagactttc ctcgtcgaga ctctcatcct gcttcaacca
    16261 ttgctgcaac ggcagttcca ggcccagttc gcctgcaagc gtccgttcga ggccgggaac
    16321 gtcccactgc tcctccacgc tgtccggcgg aatatgctgc gatacgagcg attcgaccgt
    16381 gccttcgcgc atcgaacgga tggtttccga gatgtcggtc gcttcgagca gttcattgcg
    16441 ctgctggtag atcaccttgc gctggtcgtt ggagacgtca tcatattcca gcaactgctt
    16501 gcggatgtcg aagttgcgcg cctcgacctt acgttgcgcg ttctcgatcg cgcgcgtgac
    16561 ccagggatgc tcgattgcct cgccgtcggg cattttcagc cggtccatga tcgcagccac
    16621 ccggtccgag gcaaagatgc gcagcagcgg atcctcgagg gccaggtaaa agcggctcga
    16681 gcccggatcg ccctggcggc ccgaccgccc gcgcaactgg ttgtcgactc gccgcgactc
    16741 gtgccgctcg gtgccgatga tgtgcaggcc gccggcgcca accacctgct gatgcagctt
    16801 ctgccagcgg tcgcgcatct cggcgacgcg cttttccttt tcctgatccg gaaggctggc
    16861 gtcgttgctg accttgttga tctcgggttc gggatttccg cccagcacga tgtcggttcc
    16921 gcggcccgcc atattggtgg cgattgtaac catcttgggc cggccagcct gggcgatgat
    16981 ctccgcttcg cgtgcatgct gcttggcgtt cagtacctgg tgcggcagct tggctttttc
    17041 gagttgtgcg gccagcaatt ctgagttctc gatcgaagtg gtgccaacca gaaccggctg
    17101 gccgcgctcg tagcagtcct tgatgtcgtt gatcaccgcc tggtacttct cgcgcgcagt
    17161 ccggtagacc tggtccatgc ggtcttcgcg gatcatcggc atgtgggtgg gaatgatcat
    17221 cgtttccagc ccgtagatct gctggaattc gtaggcttcc gtgtcggcgg tgccggtcat
    17281 gcccgccagc ttcttgtaca tccggaaata attctggtac gtgatcgagg cgagcgtctg
    17341 gttctcgcgc tggatgcgca caccctcctt ggcctcgatc gcctggtgca agccatcgga
    17401 ccagcgccgg ccgggcgtca accgtccggt gaattcatcg acgatgatga tctcatcgtt
    17461 ctgagccacg tagtgctggt cgcgatgaaa cagcgagtgc gcccgcaatc cggcgtacag
    17521 atggtgcatg agggcgatgt tggcggcgtc gtacaagctg ccgttctccg gcaacagccc
    17581 ggccttggtc agcagatctt cggcgcgctc gtgaccggtc tcggtcaaca gcacctgatg
    17641 cgccttttcg tccacggtga agtcgccggg gccgttctcc tccttgaccc gggtgagctt
    17701 gggaacgatc tcgttcatcc ggtaatacag gtcggtactg tcatccgcct ggccggaaat
    17761 gatgagcggc gtccttgctt cgtcgatcag gatcgagtcg acttcgtcga cgatcgcata
    17821 ggtcaggccc cgctgcaccc gctcactggc ctggaacacc atgttgtcgc gcaggtagtc
    17881 gaagccgaac tcgctgttgg tgccgtaggt gatatcggca gcataa
//
LOCUS       DASQOP010000011         6750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_4803, whole genome shotgun sequence.
ACCESSION   DASQOP010000011 DASQOP010000000
VERSION     DASQOP010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6750
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4803"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="VE030_00420"
     CDS             <1..141
                     /locus_tag="VE030_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HZF21908.1"
                     /translation="PERYALKARDGILNPDAIAENYWQLHRQHRSAWTHELDLRPWIE
                     KW"
     gene            167..1102
                     /locus_tag="VE030_00425"
     CDS             167..1102
                     /locus_tag="VE030_00425"
                     /inference="COORDINATES: protein motif:HMM:NF014867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HZF21909.1"
                     /translation="MKVGIVGAGSVGSACLHSLVLRGPAREIVVVNRNRARAKGLITD
                     LQYGALLAPPVELRDGDYADLSGAALVMITAGINEKTGGATDRSDPAGRLRLLDTNAV
                     LYRDIVAKVAAAAPQAIILVVTDPPDPLADLARLVAGHERVLSTGTFLDSLRFRFHLG
                     RRLKVSPKSVDAQVLGEHGTSQVFLWSSARVAGMPIQCAFDSSCGTGDELRRDVEQEV
                     RYANITIIEGTGASQLGIGVAAARITEAVLRDEQVVIPIGSFIPKYGVTLSLPSVVGR
                     RGVSRILEPEMSEDERQGLQRSAEVLRNAVARIKT"
     gene            complement(1224..2705)
                     /locus_tag="VE030_00430"
     CDS             complement(1224..2705)
                     /locus_tag="VE030_00430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZF21910.1"
                     /translation="MNDQDRFRARGFFGLRRLAVPALMLVLAGCAAVGPDYRRPETGA
                     PKAWKESDQQAATAKTLPAAWWEVFGDAKLNELEDQAKFQNPNLRAAAARVMQARAIA
                     RVSEADFYPNVTLDPAASRDRYSENRPVQPNTPVVGYTASHFRLPLDASYEIDIWGRV
                     RRAVEAADARLDASADEYFTVLLTLQADIAQNYFALRSLDAERDVLRRAIGIRRQALE
                     LIRVRYEGGIGTELDVTRAQTEVASAESESIGVSRRRAEIEHAIAVLIGKPPADFALA
                     ENPLNLAPPAIPAGLPSELLVRRPDVAQAERLLAARNAEIGVAKAAYFPSIRLTGALG
                     FDSAELGDLVKSGSGAGSLGVGLSLPVFDGGRIKGNVERAKAAYEENLAQYRSRVLGA
                     FGDVEDALSGLRILAEQAGAQARALASAQQTAEISATRYEAGLVIFLEFVDAERTRLA
                     TQRLATQIDGQRLVASVGLIKALGGGWQEENHRAVLGPRAAAN"
     gene            complement(2702..3898)
                     /locus_tag="VE030_00435"
     CDS             complement(2702..3898)
                     /locus_tag="VE030_00435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZF21911.1"
                     /translation="MEHDSTEEITKALAQRRGTGSGRHRNVAIAVAAGALVLTGLFGI
                     GLIPRLQTQKAVADIAKDNHPIVSVIPAMRGNAATEVNLPGTLLPYQETPIYARTNGY
                     VKRWHVDIGAKVANGELLAEIETPEVDRELKQAVANLAQVRAHLELDRTSAERWQTLL
                     TRKGVSQQEVDEKVGAYQARKADYAAAEANVQRLEEMKLFQRVAAPFSGTITARNVEV
                     GQLINAGANDPARWMYKLAKTGTLRLYVNVPQNSMRLIQPGASVDVILQEFPGKAFPG
                     KVARTAGALDAQSKTLLTEVQVPNDKDELLAGMYAQVRFKVNQAQPTIILPSNTLIMR
                     ADGPQVAAVASDVVHMRKVTLGRDFGQQIEIIAGLDEKEQVVTNPPDFLREGVVVKAI
                     APEQKK"
     gene            complement(3925..>6750)
                     /locus_tag="VE030_00440"
     CDS             complement(3925..>6750)
                     /locus_tag="VE030_00440"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZF21912.1"
                     /translation="RQAIAPVKGSRLPLPFGGKPRQIMVDLDPQALNARGLNPMDVTN
                     AFAAQNLSLPTGSAKIGTKEYMVGLNSNPENIAAMNDVPIKNVNGSMVYVRDVANVRD
                     GFGVQTNVVRSEGRRGALLTVLKTGGASTLDVVQGIKDALPALRATYPDIEMKELFDQ
                     SSFVKAAVDGVVLEGLIAGLLTAAMILLFLGSWRSTLIVTVSIPLSILTSLIILALTG
                     ETLNIMTLGGLALAVGILVDDATVAIENIHRHVEMGKPLEQAIIDGAQQIAVPAFVST
                     LAICIVFVSVVFLTGPAQFLFTPLALAVVYAMLASYVLSRTLVPVMAKYLLHGEKHGG
                     AADGGAQHGGTRRGGVFSRIHHGFEAGYEGLRGRYVRSLDWGLNNRVAVFALFAVVGL
                     ASLAVVPFIGRDFFPQVDAGQIRMHVNAPAGTRLEETEEVFTRVQRAIREILPPEDIA
                     LSMDNIGQPQPVNMAFVDTPAISAADGEILISFKPERHKSTSEYVELLRAQLPKRFPD
                     LTFFFQPADIVNQILNFGLPSPVDVQIAGYNPKLYGIARELEQKLKGIPGAVDVHLHQ
                     VLNAPALQVTVDRSRAAELGLTQRDVANNLLVALSSSSIVNFNLWPDPKTGVTYPIAV
                     QTPQYRLDSIEDMMNTQLPGRGSVNMQMLSNVATVERRETPAVVSHYDIQPVFDVFAN
                     VQGRDLGSVSDEIDKVLAEYLPAKPPMWKSVLCPKDPGMLTSWMCAKPETAKGPDGKP
                     VEPKLPPGTKLTVRGQVDSMNTAFTRMGYGMIFAALLIYFLMVVNFQSWTDPFIIITA
                     LPGALCGIAWMLFMTHTTFSVPSLMGAIMCLGVATANSILIVTFANEQLRAGKSAHDA
                     ALAAGFTRLRPVLMTASAMIIGMVPMSLGLGEGGEQNAPLGRAVIGGLIFATISTLFF
                     VPTVFTLIRERLHHSPEPQLNTAPQAG"
BASE COUNT         1118 a         2138 c         2262 g         1232 t
ORIGIN      
        1 ccggagcgct acgcactgaa ggcccgggac ggcatcctga acccggacgc gatcgcggag
       61 aactactggc agttgcatcg gcagcatcgc agcgcctgga ctcacgagct cgatttgcgg
      121 ccgtggatcg agaagtggtg aggcgagagg aattggagcc ccgaccatga aagtgggcat
      181 cgtcggtgcg ggctccgtag gctcggcctg tcttcactcg ttggtcctgc ggggacccgc
      241 gcgcgagatc gtggtcgtaa acaggaatcg ggcgcgcgcg aagggattga ttaccgacct
      301 gcagtatggc gcgctactgg cgccgcccgt cgaactccgc gatggcgact atgccgacct
      361 gtccggtgcg gcattggtca tgatcaccgc cggcatcaac gagaagaccg gaggtgcgac
      421 cgaccgcagc gatcccgcgg ggagattgcg gctactcgac accaatgcgg tgctgtatcg
      481 ggacatcgtt gccaaagtcg ccgccgcggc accgcaggcg ataatcctgg tcgtgaccga
      541 tccgccggat ccgctggccg atctcgcgcg cttggtcgcc gggcacgagc gggtgctgag
      601 cacgggcact ttcctcgact cgctccgctt tcggttccat ctcggccggc ggctgaaagt
      661 gagtccgaaa tccgtggatg cccaggtact cggcgagcac ggcacgtcgc aagtgttcct
      721 ctggtcttca gcacgcgttg caggcatgcc gatccaatgt gcgttcgatt cgagctgcgg
      781 gaccggcgat gaattgcgcc gggacgtcga gcaggaagtc cgttatgcga acatcacgat
      841 catcgaagga accggcgcca gccagttggg aatcggcgtt gccgctgcgc gcattaccga
      901 ggcggtcctg cgcgacgagc aggtcgtcat tcccatcggc tcgttcattc caaagtacgg
      961 cgtcaccctg tcgctgccga gcgtggtcgg ccgccggggc gtgagccgca ttctcgagcc
     1021 ggaaatgtcg gaagacgagc gccaaggtct ccagcgcagc gcggaggttt tacggaacgc
     1081 cgtggccagg attaaaacct agagcagagt ggtgaaagta cgcaaaggaa aactgaatgg
     1141 cagggaaaag ggaaaagtaa ctgcgccttg ccggagaatg tctttacttt gcccattccc
     1201 cattcctcaa tctcttcgaa ctttcagttc gccgcagcgc gtggccccag taccgcacgg
     1261 tgattttctt cctgccaacc gccgccgagc gccttgatca ggccgacgct cgcgaccaag
     1321 cgctgaccgt cgatctgggt ggcgagccgc tgcgtggcaa gccgggtgcg ttcggcatcg
     1381 acgaactcca ggaaaatcac caggccggct tcgtatcgtg ttgcggagat ctcggccgtc
     1441 tgctgcgcag aggcgagtgc acgggcctgc gcgccggcct gctccgcgag tattctcagg
     1501 ccggagagcg cgtcttccac atccccgaat gcgccgagca cgcggctgcg gtattgcgcc
     1561 aggttttcct cgtaggcggc cttggcacgc tccacgttgc ccttgatgcg tccgccatcg
     1621 aagaccggca gcgatagacc gactccgagg gatccggcgc cgctgccgga cttcaccagg
     1681 tcacccagtt ctgcgctatc gaagccgagc gcgccggtga gccggatgct cggaaagtac
     1741 gccgccttgg cgacgccgat ctcggcgttg cgcgcggcca gcagccgttc cgcctgtgct
     1801 acgtcgggac ggcgcaccag gagctcggag ggcagtcctg ccggaatggc cggcggcgcg
     1861 aggttcagcg ggttctcggc gagcgcgaaa tcggcgggcg gtttgccgat caggaccgcg
     1921 atcgcgtgct cgatctcggc gcggcgtctg ctgacaccga tagattcgga ttccgccgag
     1981 gcgacctcgg tttgcgcgcg cgtcacgtcg agttcggtgc cgatgccgcc ttcgtagcgg
     2041 acgcggatca gctccagcgc ctggcggcgg attccgatgg cacggcgcag cacatcccgt
     2101 tcggcgtcga gcgagcgcag cgcaaagtag ttctgcgcga tgtcggcctg cagggtcagc
     2161 agcaccgtga agtattcgtc ggcgcttgcg tccaggcggg cgtccgcggc ttcgaccgcg
     2221 cgtcgcacgc ggccccagat gtcgatttcg taggaggcat ccagcggcag ccggaaatgg
     2281 ctcgccgtat agccgacaac cggcgtgttc ggctgcaccg gacggttttc cgagtaacga
     2341 tcgcgcgagg cagccgggtc gagtgtcacg ttcggataga agtccgcctc cgatacacgc
     2401 gcgatcgcgc gcgcctgcat gacccgcgcc gcggcggccc gcaggttcgg gttctggaac
     2461 tttgcctgat cttccagctc gttcagcttc gcgtcgccga acacttccca ccatgcggcc
     2521 ggcaatgtct tggcggttgc agcctgctgg tcggattctt tccatgcctt gggcgcgccg
     2581 gtttcggggc gcctgtaatc cggtccgacc gcggcacagc cggccagcac cagcatcagc
     2641 gccggcaccg cgagccggcg caggccgaag aaaccgcgtg cgcgaaacct atcctggtcg
     2701 ttcatttttt ctgttccggt gcaatcgcct tgacaaccac gccttcgcgc aggaagtcgg
     2761 gcgggttggt aaccacctgt tccttctcgt cgaggccggc gatgatctcg atctgctggc
     2821 cgaaatcgcg gcccaaggtc accttgcgca tgtgcacgac atcgctggcc actgcggcga
     2881 cctgcgggcc gtcggcacgc atgatcaggg tgttggacgg caagatgatg gtgggctggg
     2941 cctggttcac tttgaagcgc acctgggcgt acatgccggc cagcaattca tccttgtcgt
     3001 tgggcacctg cacttcggtc agcagcgtct tcgactgcgc gtcgagcgcg ccggcggtgc
     3061 gagcgacctt gccggggaag gccttgcccg ggaactcttg caggatcacg tccaccgacg
     3121 caccaggctg gatcaggcgc atggagtttt gcggcacgtt gacgtacagc cgcagggtgc
     3181 cggtcttggc aagcttgtac atccagcgcg ccggatcgtt ggcgccggcg ttgatcagtt
     3241 ggccgacttc cacattgcgc gcggtgatgg tgccggagaa gggcgcggcg acgcgctgga
     3301 acagtttcat ttcctccagg cgctgcacgt tggcttcagc cgccgcgtag tccgccttgc
     3361 gtgcctggta cgcgccgacc ttctcgtcga cttcctgctg ggatacgccc ttgcgggtga
     3421 gaagcgtctg ccagcgctcg gcggacgtgc ggtccagttc caggtgcgct ctcacctgcg
     3481 cgaggttggc taccgcctgc ttgagttcgc ggtccacttc cggggtctcg atctcggcca
     3541 gcaattcgcc gttcgcgact ttggcgccga tgtccacgtg ccagcgcttc acgtagccgt
     3601 tggtgcgcgc gtagatcggc gtttcctggt aagggagcag cgtgcccggc aggttcactt
     3661 cggtggcggc attgccgcgc atcgccggga tgaccgaaac gatcggatgg ttgtccttcg
     3721 cgatgtccgc cactgctttt tgtgtctgca gccgcgggat cagtccgatg ccgaacaatc
     3781 cggtcaacac cagcgccccc gcggccaccg cgatggctac attgcgatga cggccgctgc
     3841 cggttccccg gcgctgcgcc agggctttcg tgatttcttc agtggagtcg tgttccatgg
     3901 ctctggacct ctatgtcggg tggatcaacc ggcctggggc gcagtgttca actgcggctc
     3961 gggcgagtgg tgcaggcgtt cgcggatcag cgtgaacacc gtgggtacga aaaacagcgt
     4021 ggaaatggtc gcgaaaatca ggccgccgat gacggcgcgg ccgagcggcg cgttctgctc
     4081 gccgccttcg cccaggccca gcgacatcgg caccatgccg atgatcatcg ccgaagcggt
     4141 catcagcacc ggccgcagcc gcgtgaagcc ggcggcgagc gcggcatcgt gcgcggactt
     4201 gccggcgcgc agctgctcgt tggcgaaagt gacgatcagg atggagttcg cggttgccac
     4261 acccaggcac atgatcgcac ccatgagcga gggcacgctg aacgtcgtat gcgtcatgaa
     4321 cagcatccag gcgataccgc acaaggcgcc gggcagcgcg gtgatgatga tgaaggggtc
     4381 ggtccacgac tggaagttca ccaccatcag gaagtagatc agcagcgcgg cgaagatcat
     4441 gccgtagccc atgcgcgtaa aggcggtgtt catgctgtcc acctggccgc gcaccgtaag
     4501 cttggtgccc gggggcagct tcggctcgac cggtttgccg tccggacctt tggcggtctc
     4561 cggcttcgcg cacatccacg aggtcagcat gcccggatct ttcgggcaga gcacgctctt
     4621 ccacatcggc ggcttggccg gcagatattc ggcgaggacc ttgtcgatct cgtcggacac
     4681 ggagcccagg tcgcggccct ggacgttggc gaacacatcg aacaccggct ggatgtcgta
     4741 gtggctcacc actgccggtg tttcgcgccg ctccaccgtc gccacgttgg agagcatctg
     4801 catgttcacc gatccgcgcc cgggcaactg ggtgttcatc atgtcttcga tcgagtcgag
     4861 ccggtactgc ggcgtctgca ccgcgatcgg gtaggtgacc ccggtcttcg gatccggcca
     4921 caggttgaag ttcacgatcg agctcgagga cagcgccacc aaaagattat tggcgacgtc
     4981 gcgttgcgtg agccccagct cggcggcgcg cgaacggtcc acggtgacct gcagtgccgg
     5041 tgcattcagg acctggtgca gatgcacgtc cacggcgccg gggatgccct tcaacttctg
     5101 ctcgagttcg cgggcgatgc cgtagagctt cgggttgtag ccggcgatct gcacgtccac
     5161 cggcgaaggc aggccgaagt tcaggatctg gttgacgatg tccgccggct ggaagaagaa
     5221 ggtgaggtcg gggaagcgct tcggcagttg ggcgcgcagc agctccacgt actctgaggt
     5281 ggacttatgc cgctcgggct tgaacgaaat caggatctca ccgtccgcgg cactgatcgc
     5341 cggggtgtcc acgaaggcca tgttgaccgg ttggggctgg ccgatgttgt ccatcgaaag
     5401 cgcgatgtct tcgggcggaa ggatctcgcg gatggccctc tgcacccggg tgaagacctc
     5461 ctcggtttcc tccaggcgcg tgccggcggg agcgttcacg tgcatgcgga tctgcccggc
     5521 gtccacctgc gggaagaagt cgcggccgat aaacggcacc accgccaggc tggcgaggcc
     5581 caccacggcg aacaatgcga acaccgcgac gcggttgttg aggccccagt ccagcgagcg
     5641 tacgtagcgt ccgcgcaggc cttcgtagcc ggcctcgaaa ccgtgatgga tgcgcgagaa
     5701 cacgccgccg cgccgggttc cgccatgctg cgctccgcca tctgccgcac cgccgtgttt
     5761 ttccccgtgc aggaggtact tggccatcac cgggaccagt gtgcgcgaca gcacgtacga
     5821 ggcgagcatc gcgtatacca ccgcgagcgc gagcggcgtg aacaggaatt gggccgggcc
     5881 ggtgaggaac accaccgaga cgaacacgat acagatcgcc agggtggaga cgaaagcggg
     5941 taccgcgatc tgttgcgcgc cgtcgatgat ggcctgctcg agcggcttgc ccatctccac
     6001 gtgacggtgg atgttttcga tcgccaccgt ggcatcgtcc accaggatgc cgactgcgag
     6061 cgccagtccg ccgagcgtca tgatgttgag cgtctcgccg gtcagcgcca ggatgatgag
     6121 cgaggtcagg atcgacagcg ggatcgacac cgtcacgatc agggtgctgc gccagctgcc
     6181 gaggaacagc aggatcatgg cggcggtcag cagcccggcg atcaggcctt cgagcaccac
     6241 gccgtccacc gccgccttca cgaacgaaga ctggtcgaac aattccttca tctcgatgtc
     6301 gggataagtg gcgcgcagcg cgggcagcgc gtccttgatc ccctgcacca cgtccagcgt
     6361 cgaggcgccg ccggtcttga gcacggtgag caaggcgccg cgccggcctt cgctgcgcac
     6421 gacgttggtc tgcacgccga agccgtcgcg cacgttggcg acgtcgcgca cgtacaccat
     6481 cgagccgttg acgttcttga tcggcacgtc gttcatggcc gcgatgttct cggggttgct
     6541 gttcagcccg accatgtact cctttgtgcc gatcttggcg ctgccggtgg gcaggctcag
     6601 gttctgcgcg gcgaacgcgt tggtgacatc catcgggttg aggcccctgg cgttcagggc
     6661 ctgcggatcg aggtccacca tgatctggcg cggcttgccg ccgaagggca acggcaggcg
     6721 cgagcccttc accggggcga tggcctggcg
//
LOCUS       DASQOP010000012         4697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1414357, whole genome shotgun sequence.
ACCESSION   DASQOP010000012 DASQOP010000000
VERSION     DASQOP010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414357"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..1347)
                     /locus_tag="VE030_00445"
     CDS             complement(52..1347)
                     /locus_tag="VE030_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZF21913.1"
                     /translation="MRRLALLAPLLLIAAMPAPAQDLATTVDQKVRALEPKIIAWRRD
                     IHQNPELSNREVRTSKLVADQLRKLGIEVKTGVAKTGVVGLLKGGKPGPVVALRADMD
                     ALPVTEESDLPFKSAVKTEYLGQPVGVMHACGHDAHVAILLGVAEALAGVRDLIPGTV
                     KFIFQPAEEGPPPGETGGAPQMVAEGVLENPKPSAIFGLHVISGIPAGMITYRPGPLM
                     ASADWLYITVKGKQTHGAWPWSGIDPVVTSAQVVTGLQNIVARQIDVSREPAVVTVAT
                     IHGGTRKNIIPDQVEMTGTIRTFDEGMRQDVHARIKHIAESIAEANHAHADVKVEKAV
                     PVTVNDPALTEKMIPTLKRIGGDGGSRPQQRVMVAEDFSYFQEKVPGLFYFVGVTPKD
                     QDMAAAAPNHSPRFHIDESGLLQGARSLAALAVDFLSSR"
     gene            1515..2036
                     /locus_tag="VE030_00450"
     CDS             1515..2036
                     /locus_tag="VE030_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HZF21914.1"
                     /translation="MTNIKLTVAFAAAMVLIVLSSNVLVQYPLSDWLTWGAITYPFSF
                     LLVDLANRYHGPCMARRIVYVGFVVALVLSAWLATPRIAVASGTAFLCAELLDVTVFN
                     RLRRHAWWKPPLVSTLFASVLDTAVFFTLAFAGTDMPWITLALGDLGVKLAIALFALV
                     PFRVALALVRPSA"
     gene            2142..3155
                     /locus_tag="VE030_00455"
     CDS             2142..3155
                     /locus_tag="VE030_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ionic transporter y4hA"
                     /protein_id="HZF21915.1"
                     /translation="MAASIAMPGAFMWGLAAVALVGAVIAAVHHAEVVAHRVGEPFGT
                     LVLAVAVTVIEVALIVSVMLSGGAGKEALPRDTVFAAIMICCNGIVGLCLLIGGVHHR
                     EQGFQIQGASAALAVLCALSVLSMILPSYSITVPGPGLSTSQLVFSGIVSLVLYMVFV
                     FVQTIRHRDYFLAESDDEDAHALPPSGRTALASLALLIAALIAVVGLAKILSPVVEGG
                     VNRMGAPAAVVGIVIAMLVLLPEGSAALRNARANRLQTSLNLALGSALASIGLTIPTV
                     AVVSILIGQPILLGLMPKEIVLLALTLIVGVITLGTGRTTLLQGVVHLAIFAAFLFFA
                     IVP"
     gene            complement(3378..4376)
                     /locus_tag="VE030_00460"
     CDS             complement(3378..4376)
                     /locus_tag="VE030_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HZF21916.1"
                     /translation="MARNMQVLLASRPTGWVEESNFKIVETDVPKPGPGEVLVKNLWL
                     SLDPYMRGRMNAAKSYAKYVEIGEVMTGGTVGQVVESGNPKFKVGDYVVGSLGWQLYA
                     ISNGEGLAVVDPKLVPLTAYLGVCGMPGGTAWIGLLEHCAPKVGETVLVSAATGAVGG
                     VVGQLAKLQGCRAVGIAGGPKKCEFAVKELGFDACVDYKAGKLLDDLKAACPNGVDNY
                     FENVGGEVLDAAFKVLNPFSRVALCGLIAEYNATEPYGVKMMRSLLVNRVKLQGFIVF
                     DRVDLYQRAIPKLANLVSQGKIKYRETIAEGLQNAPRAFIGLLKGQNLGKQLVKLT"
     gene            4459..>4697
                     /locus_tag="VE030_00465"
     CDS             4459..>4697
                     /locus_tag="VE030_00465"
                     /inference="COORDINATES: protein motif:HMM:NF018717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HZF21917.1"
                     /translation="MIYALNLFNLIPGKENEYRDYSVRAGRIIFGLGGRVVSAGTGPL
                     REMHGDTTRRQLIVVEFPSLEVFEQFIDEAENQNI"
BASE COUNT          766 a         1487 c         1450 g          994 t
ORIGIN      
        1 tgagtctgga atcaggggcg cacggcaatc aaggcgttcg cggttgaagg actagcggct
       61 ggagaggaaa tccaccgcca acgcggccag cgagcgtgcg ccctgcagca gtcccgattc
      121 gtcgatatgg aagcgtggcg agtggttcgg cgcggcggcg gccatgtcct ggtctttcgg
      181 cgtcacgccg acgaaataga acaagcccgg aactttttcc tggaagtacg agaagtcctc
      241 ggccaccatc accctctgtt gcggcctcga tccgccgtcg ccgccgatgc gcttcagcgt
      301 cggaatcatc ttttcggtga gcgcgggatc gttgacggtg accggcaccg ccttttccac
      361 tttcacgtcg gcatgggcgt gattggcctc ggcgatcgac tccgcgatgt gcttgatgcg
      421 ggcgtgaacg tcctggcgca taccctcgtc gaaagtacgg atcgtcccgg tcatctcgac
      481 ctggtcgggg atgatgttct tgcgcgtgcc gccgtgaatg gtcgccaccg tcacgaccgc
      541 cggttccctg gagacgtcga tctgccgcgc cacgatattc tgcagcccgg tgacgacctg
      601 ggcggaggtc accaccgggt cgatccccga ccacggccac gcgccgtggg tctgcttgcc
      661 tttcacggtg atgtagagcc agtctgcgct cgccatcagc ggtccggggc gataggtgat
      721 catgcccgca gggattcctg aaatgacgtg caggccgaaa atggccgaag gcttcgggtt
      781 ctccagcacg ccttcagcaa ccatctgcgg cgcgccgccg gtttcgccgg gcggcggtcc
      841 ttcctccgcg ggctgaaaaa tgaacttgac cgtccccgga atcagatcgc gcacacccgc
      901 caatgcctcg gctacgccga gcaggatggc cacgtgcgca tcgtgtccgc aggcatgcat
      961 gacgccgacc ggctggccga gatactcggt cttgaccgcg gacttgaacg gcaggtccga
     1021 ttcctcggtt accggcagcg cgtccatgtc ggcgcgcagc gccaccaccg gtcccggttt
     1081 gccgcccttg agcaggccga ccacgccggt tttcgccacg ccggtcttga cctcgatgcc
     1141 gagcttgcgc agttgatccg ccaccagctt cgacgttctc acctcgcggt tggagagttc
     1201 ggggttctgg tgtatgtcgc ggcgccaggc gatgatcttc ggttccagtg cgcgaacctt
     1261 ctgatcgacg gtggtggcca ggtcctgcgc cggggcaggc attgcagcaa tcaacagcag
     1321 cggggcgagt agcgccagac gacgcatgaa cggactcctt cgaaagtcgg tgtgttcctg
     1381 tgaacgggtg caggcgcaat gatacggcca gccgcccggt tcggaatagt aatccgcgga
     1441 gtgctggcaa cttccgcggc gcaaccccat aatcgcttcc cgaatccatt ctccgatatc
     1501 cgcaaagggc agtcatgacg aacatcaagc tgaccgtcgc atttgccgct gcgatggtcc
     1561 tgatcgtgct gagctcgaac gtcctggtcc agtatccgct cagcgactgg ctcacctggg
     1621 gcgcgatcac ctatccgttc tcgttcctgc tcgtagacct cgcgaaccgc tatcacggtc
     1681 cgtgcatggc gcgaagaatc gtgtatgtcg gcttcgtggt cgcgctggtg ctgtcggcgt
     1741 ggctggccac gccgcgcatc gccgttgcgt cgggaacggc gttcctgtgc gccgaactgc
     1801 tggacgtgac ggttttcaac cggctgcgcc ggcacgcatg gtggaagcct ccgctggtgt
     1861 ccaccctgtt cgcctcggtt ctggatacgg cggtattctt caccttggct ttcgccggca
     1921 ccgacatgcc ctggatcacg ctggcgctgg gcgatctcgg cgtgaagctg gcgatcgcgt
     1981 tgttcgcgct ggtgccgttc agggttgcgc tggcgctggt tcggcccagt gcctgatgcg
     2041 gctgaccggg tcggggccgc cggcatgata cgttcggggt cgtgttgagc aagaccaatg
     2101 cgcattggac ctggttcgtg ccggtgctgg ggtggctggt catggcggct tcgatagcca
     2161 tgccgggtgc attcatgtgg ggattggcgg cggtggcgct ggtgggtgcg gtcatcgccg
     2221 cggtccatca cgccgaagtc gtggcgcacc gggtcggcga gcctttcggc acactggtgc
     2281 tcgccgtagc ggtcacggtg atcgaggtgg cgctgatcgt ttccgtgatg ctgtccggcg
     2341 gcgcgggcaa ggaagcgctg ccgcgcgaca cggtgttcgc cgcaataatg atctgctgca
     2401 atggcatcgt gggcctgtgc ctgctgatcg gcggcgtgca tcatcgcgag caaggattcc
     2461 agatccaggg cgccagcgct gcgctcgctg ttctctgcgc cttgtcggtt ctgtcgatga
     2521 ttttgcccag ctattccatc accgtgcccg gccccgggct ctcgacttcg cagcttgttt
     2581 tttccggaat cgtatcgctc gtgctgtaca tggttttcgt tttcgtccaa accatccggc
     2641 accgcgacta tttcctggcg gagagcgacg atgaagatgc gcatgcgttg ccgccttccg
     2701 gccgcacggc cctcgccagt cttgcgctgc tcatcgctgc cctgatcgct gttgtcggcc
     2761 ttgcgaaaat cctgtcgccg gtcgtcgaag ggggcgtgaa ccggatgggt gcgccggcag
     2821 cggtggtcgg gatcgtcatt gcgatgctgg tgcttctgcc ggaaggatcc gcggccttgc
     2881 gcaacgccag ggcgaaccgg ctgcagacca gcctcaacct cgcgctcggc tccgcgctgg
     2941 ccagcatcgg cctgacgatt cccacggttg ccgtcgtctc catcctgatc ggtcagccga
     3001 ttcttcttgg gcttatgccg aaggagattg tgcttctggc gctgacgctg attgtcggcg
     3061 tgatcacgct gggcacgggc cgcactacgc tgctgcaggg cgtggttcac ttggcaattt
     3121 tcgccgcatt tttgttcttt gccatcgtgc catgaatgcc cattctgatt gcaatccgga
     3181 ccgggcatgc tcggagcgct gaccatccgc caagacgcaa aggagcgcaa agaaggtcaa
     3241 aaaccccaaa acgagttttt atagactaca tgctgccctt atgtcagtta acgtggcgcc
     3301 gcgcttttta tactgtcctc cggatgtttt tctttgcgac ctttgcgtct ttgcggtgaa
     3361 aattattttc gcagtaatca ggtgagtttg acgagttgtt tgccgaggtt ctggcccttc
     3421 agcaggccga tgaacgccct cggcgcattc tgcaggcctt cggcgatggt ctcgcggtat
     3481 ttgatctttc cctgcgaaac cagatttgcg agcttcggaa tcgcgcgctg gtacaggtcg
     3541 acgcgatcga aaacgatgaa gccctgcagc ttcacccggt tcaccagcag cgaacgcatc
     3601 atcttcacgc cgtagggttc ggtagcgttg tactccgcga tcagtccaca cagcgccacg
     3661 cgcgaaaacg gattgagcac cttgaacgcg gcatccagca cctcgccgcc gacgttctcg
     3721 aaataattat ccacgccgtt cgggcacgcc gccttgagat cgtccaggag cttgccggcc
     3781 ttgtaatcca cgcaggcgtc gaacccgagt tccttgaccg cgaactcgca cttcttcggg
     3841 ccgccggcga tgccgaccgc gcggcagccc tgcaatttcg ccagctggcc aaccacgcca
     3901 ccgaccgcgc ccgtggccgc cgataccagc acggtttcgc cgaccttggg cgcgcagtgc
     3961 tccaggagcc cgatccacgc ggttccgccc ggcatgccgc acacgcccag atacgcggtc
     4021 aatggaacca gcttgggatc caccaccgcg agcccttcac cattcgagat cgcgtacaac
     4081 tgccagccga gcgagccgac cacgtaatcg ccgaccttga acttgggatt gcccgactcg
     4141 accacctggc cgaccgtgcc gccggtcatg acttcgccga tctccacgta cttcgcgtac
     4201 gacttcgccg cgttcatccg gccgcgcatg tagggatcga gcgagagcca cagattcttc
     4261 accagcactt cgccgggtcc gggtttcgga acatcggttt cgacgatctt gaagttcgat
     4321 tcctcaaccc agccggtggg ccggctggcg agcagcacct gcatgttcct ggccatgttg
     4381 actccctgat gttgtgtcaa ttcgtatagc attctaccgg acgcgactcc tccgaccctg
     4441 tcattcccgg atgctgccat gatctatgcg ctgaaccttt tcaatctcat cccgggcaag
     4501 gagaacgagt accgcgacta cagcgtcagg gccggcagga tcattttcgg cctgggcggg
     4561 agggtggtga gcgccggcac cggtccgctg cgcgagatgc acggcgatac cactcgccgc
     4621 cagctgatcg tggtcgagtt tcccagcctg gaagtgttcg aacagttcat cgacgaggcg
     4681 gagaatcaga acattca
//
LOCUS       DASQOP010000013         4045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2018934, whole genome shotgun sequence.
ACCESSION   DASQOP010000013 DASQOP010000000
VERSION     DASQOP010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2018934"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VE030_00470"
     CDS             complement(<1..339)
                     /locus_tag="VE030_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF21918.1"
                     /translation="MPTRLSFPARKPPLGSAPGPVPAPGNESTTADPGRGNDWNGTGP
                     ATAQRPRGVLNERRGDNAATDAWFWHGFVSDFLPQMVWTAGPDGSVDFCNRHWTEYSG
                     LLREDSRGNGW"
     gene            complement(449..1963)
                     /locus_tag="VE030_00475"
     CDS             complement(449..1963)
                     /locus_tag="VE030_00475"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZF21919.1"
                     /translation="MPNEDRSREYSDLSPELLEIVRALQTSLTEKRVRQQLQAVDSKP
                     SMLESALSRQQARRATQPQIASHPQLLAAVVETADAAIISETWNGVVLTWNRGAERIF
                     GYSADEIVGKSRACLIPEFLKNEDAKIMRQVIDGKATLHCETVWQHKDGKFIDVAVSL
                     SPVVENDKVVRLSLVATDATERKRMERALMESERKQRQRTSELKILLDAVPAAVWIAH
                     DAECKLITGNRTSYDLLRLPQGSNISFTGPEETRPVHFRIFRNGRTLEPHEMPVQMAA
                     FRGIEVRDFEEDLVFDDGTIKHLIGNAAPLRDTSGKVYGAVAAFIDITARRNMEEQIR
                     RMAHFDSLTSLPNRILLMDRLEQALAISQRNQSKTGVIFMDLDNFKEVNDKLGHHVGD
                     MMLQQVAERIRASLREVDTVSRLGGDEFVVVLPELRQIEDARIIGQKLLRAVDGEYIV
                     SGEKLLITPSLGISIYPDHADDAGTLIRIADKAMYHAKQSGRNTYRFYDASMKA"
     gene            2390..3844
                     /locus_tag="VE030_00480"
     CDS             2390..3844
                     /locus_tag="VE030_00480"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF004816.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZF21920.1"
                     /translation="MEINPFSSATELLHRLRDGEVSSRELLEGYLQRVERHNPGLSAI
                     VTLDAERARSRADAADARSSAGGAVPALHGLPGTIKDSFETANLRTTCGAPVYSKHVP
                     AANADAVQRLEDAGGLVFGKSNVPIHAGDLQSYNVLFGATNNPWDASRTSGGSSGGAA
                     AAVAAGLTAFELGSDIGGSIRIPAHFCGVYGHKASYGAIPLRGHIPPPPGSLSAPDMA
                     VAGPIARSAEDLGLLLGVLADAGGPVVSGWKPVLPAPRARRLQDFRVVLWLEDPALPV
                     DDEVSTAILGAVQALRKAGVKVDEAARPGITLAENFDYYLRLLWPATTAQLSAKAFAR
                     VQEAGRGADPDGVHGRLALYSGAGHREWLRLNEKREVIRARWRAFFERYDVLLCPVGP
                     VCAFAHDHSEDLIARTIVVNGQRRWYWEQLAWISLATLAYLPATAAPIGRTPGGLPVG
                     MQVIGPYFEDRSCIEFAKLLADVAGGFEAPPGFS"
     gene            complement(3864..>4045)
                     /locus_tag="VE030_00485"
     CDS             complement(3864..>4045)
                     /locus_tag="VE030_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008161937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HZF21921.1"
                     /translation="VKGDLVFFDQEGKKSSHVGIYLGDGRFVHAPSTGGKVRADKIDL
                     TYWRKHFNEARRIRM"
BASE COUNT          666 a         1243 c         1327 g          809 t
ORIGIN      
        1 ccatccgttg ccgcggctgt cttcgcgcaa caggcccgaa tattcggtcc agtggcggtt
       61 gcagaaatcg accgacccgt cgggccccgc ggtccaaacc atctgcggca ggaaatcgga
      121 aacgaatccg tgccagaacc aagcgtccgt cgccgcattg tcgcctctgc gttcgttgag
      181 gacgccgcgc gggcgctgtg cggtcgccgg cccggtgccg ttccagtcgt ttccacgccc
      241 cgggtcggct gtggtggatt cgttgcccgg tgccgggacg ggcccgggag cgctgccgag
      301 gggaggtttt cgggcgggga atgaaagtcg cgtcggcatc gttgctcctc ctttgagaaa
      361 cgggaacggg acggcctggc cttgctgtat taactccggg gaaaggcgcc aacccgccgg
      421 gggtggcttg aggatactac aagcagcgtc aggccttcat gctggcgtcg tagaaccgat
      481 aggtatttcg gcccgactgc ttggcgtgat acatcgcctt gtccgcgatt cggatcagcg
      541 tgccggcgtc gtctgcatga tcggggtaga tgctgattcc caggctgggg gtgatcagca
      601 acttctcccc ggagacgatg tattcgccgt ccaccgcccg cagcagcttc tgtccgatga
      661 tgcgtgcgtc ctcgatctgg cgcaactccg gcagcactac cacgaattcg tcgccgccca
      721 accgcgaaac cgtgtccacc tcgcgcaggc tggcgcggat gcgctccgcc acctgctgca
      781 gcatcatgtc gccgacgtga tggccgagct tgtcgttgac ctccttgaag ttgtcgaggt
      841 ccatgaagat cacgccggtc ttggattggt tgcgctggga gatcgccagc gcctgttcca
      901 ggcggtccat cagcaggatc cggttgggca ggctggtgag cgagtcgaag tgcgccatgc
      961 gccggatctg ctcttccatg ttgcggcggg cggtgatgtc gatgaatgcg gccaccgcgc
     1021 cgtagacctt gccggaggtg tcgcgcagcg gcgcggcatt gcctataagg tgcttgatgg
     1081 tgccgtcgtc gaagaccagg tcctcctcga aatcgcgcac ttcgatgccg cggaatgccg
     1141 ccatctgcac cggcatctcg tgcggttcga gcgtgcggcc gttgcggaaa atgcggaaat
     1201 gcaccggccg cgtttcctcc ggaccggtga atgagatgtt gctgccctgg ggcaggcgca
     1261 acaggtcgta agaagtgcgg ttaccggtga tcaacttgca ttcggcatca tgggcaatcc
     1321 atacggccgc gggcaccgca tcgaggagga tcttgagttc cgacgtgcgc tgccgttgct
     1381 tgcgctcgct ttccatgagc gcgcgctcca tgcgcttgcg ttcggtggcg tcggtggcga
     1441 ccagggacaa ccgcaccacc ttgtcgtttt cgacgaccgg ggacaggctg acggcgacat
     1501 cgatgaattt tccgtccttg tgctgccaga cggtttcgca atggagagtt gccttgccgt
     1561 caatgacctg gcgcattatc ttcgcgtctt cgttcttgag gaactcgggg atgaggcagg
     1621 cacggctctt acccacgatt tcgtcggcgc tgtacccgaa gatgcgctct gcgccccggt
     1681 tccaggtgag aacgacgccg ttccaggttt cgctaatgat ggcggcgtcc gcggtctcca
     1741 ccacggcagc tagcagctgg gggtgagagg cgatctgggg ttgggtggcg cgcctggcct
     1801 gctgacgaga tagtgcggac tcgagcatcg agggcttgct gtccaccgcc tgcaattgct
     1861 gacgcacgcg cttctcggta agcgacgtct gcagtgcccg tacgatctcc agcaactccg
     1921 gactgaggtc ggaatattcc cgcgaacggt cctcgttggg cacctcggcc tccccgaact
     1981 gcctgctgct tatgcttata gacacgaaca aaggtgaatc ttataggtct tatagcactc
     2041 aattgagcca cgattccgac gctgccgcaa ccgcccgcgc cagacgcggg attccgggcc
     2101 ggtcaggccg tcgagcgttg cggaatggtc ggccgaccct ggcggatcta ggcgatcgcc
     2161 gatgccacac aaacccttat ggccaagcgt taaccgccag ggacgccaag gaaaggcgtg
     2221 aaggcgaggg taacgggcgc gtcgaaacgc agatacttat acgggaattt aacacccacc
     2281 cgattcttgt gttgggcttt ccttgcgcca ttgactttcc ttggcgtccc tggctgtgaa
     2341 tggggcattg ccgaggcgtt cggaacatgc agaaaagtct gaagtatcga tggaaatcaa
     2401 tcccttctcc tcggcgaccg agcttcttca ccggttgcgt gacggtgagg tttccagccg
     2461 tgagctgctg gaaggctatc tgcagcgggt cgagcgccac aatccaggac tcagcgcaat
     2521 cgtgacgctc gatgccgagc gcgcgcgcag tcgcgccgac gccgcggatg cgcggtcctc
     2581 cgcaggaggc gccgtccccg cgctgcacgg cctgccgggc accatcaagg acagcttcga
     2641 gaccgcgaac ctgcgcacca cctgcggtgc gccggtttat tcgaagcatg tgccggcggc
     2701 caatgcggat gcggtgcaac ggctggagga tgccgggggc ctggtttttg gcaagagcaa
     2761 cgtcccgatc cacgccggcg atctgcaaag ctacaacgtt cttttcggtg ccaccaacaa
     2821 tccctgggat gcctcgcgca cctctggcgg ctcatcgggt ggtgcggcgg ccgcggtcgc
     2881 cgccggattg acggctttcg agctgggcag cgacatcggt ggctcgatcc gcattccggc
     2941 gcatttttgc ggtgtttacg gccacaaagc ctcgtacggg gctattcctt tgcgcggcca
     3001 catcccgccg ccgccgggct ccttgtcggc acccgacatg gcggtggcgg ggccaatcgc
     3061 gcgctcggcc gaggatctgg gcttgcttct tggcgtcctc gccgatgccg gcggcccggt
     3121 cgtctcaggc tggaaacccg ttttgccggc accgcgcgcc agacgcttgc aggattttcg
     3181 ggtggtgctg tggctggagg atccggcgct tccggtcgac gacgaggtaa gcacggcgat
     3241 ccttggggcc gtgcaagcat tgcgaaaggc gggagtgaaa gtggacgaag ccgcccggcc
     3301 cggaattacc ctggcggaga acttcgacta ttacctgagg ctgttatggc cggctacgac
     3361 cgcgcaactc agcgctaagg ctttcgcacg cgtgcaggag gccggccgcg gtgccgaccc
     3421 ggacggcgtg cacggccggc ttgcgctcta cagcggcgcc gggcaccgcg agtggctgcg
     3481 gctgaatgag aaacgcgagg tgattcgcgc caggtggcgc gctttcttcg agcgctacga
     3541 cgtgctgctc tgtccggtcg ggccggtatg cgcgtttgcc cacgaccaca gcgaagatct
     3601 gatcgcccgc accattgtcg tcaacggcca gcgccgctgg tattgggagc aactcgcctg
     3661 gatttcactc gccacgctgg cctatctgcc ggcgaccgcc gcgccgattg gacgtacccc
     3721 cggcggactg ccggtcggaa tgcaggtcat cggtccgtat ttcgaggacc gcagttgcat
     3781 cgaattcgcg aaattgctgg ccgatgtagc cgggggcttc gaagcgccgc cgggtttttc
     3841 atgagcggcc ggggggagca gcgtcacatc ctgatcctgc gtgcttcgtt gaagtgcttg
     3901 cgccagtacg taaggtctat cttgtccgcg cgtaccttgc cgcctgtgga gggcgcatgc
     3961 acgaatctgc cgtcaccgag gtagatgccg acgtgggagg atttctttcc ttcctggtcg
     4021 aagaacacca gatcgccttt gacaa
//
LOCUS       DASQOP010000014         4071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1214121, whole genome shotgun sequence.
ACCESSION   DASQOP010000014 DASQOP010000000
VERSION     DASQOP010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214121"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VE030_00490"
     CDS             complement(<1..671)
                     /locus_tag="VE030_00490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZF21922.1"
                     /translation="MQDKQFRRDLTALPGVQENLFQQKEQAAVILASMGDALITTDVA
                     GTIIYMNPTAERITGWRAAEAQGQPVTTVLTLVSDATREPIESIPARCVREGRAVDLD
                     DGALLVRPDGTEIAIGDSAAPLRDRDGNMAGVVLVFHDVTERRRVACQLARQGARDTL
                     TGLIGGKQFEERLARVLAQVGMAEHALCYLDLDRFKVVNDACGHEAGDELLRRIGSLF
                     YGLLG"
     gene            952..1137
                     /locus_tag="VE030_00495"
     CDS             952..1137
                     /locus_tag="VE030_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF21923.1"
                     /translation="MASNIRIAVDETTRGLTIRWFETGLERHIWVDQGTFEQFLASGE
                     TIYLDTPAIATERRAVE"
     gene            1353..1961
                     /locus_tag="VE030_00500"
     CDS             1353..1961
                     /locus_tag="VE030_00500"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELWxxDGT repeat protein"
                     /protein_id="HZF21924.1"
                     /translation="MSTTNSLRNSLLPLGVVALAAFLCGGGGDGLAAKTDGTKADTVR
                     MKSINPGAGRSGPFGLTVFNNAVYFSAFDGTSGFELWRSDGSEAGTVRVKDINPGANG
                     SIPGGFTVFNNALYFSADDGTSGFELWRSDGTEAGTVRVKDINPGAGGSIPFGFTVLN
                     NALYFFADDGTNGFEQWLSDGTEAGTMRVKDILPRTVWSGNR"
     gene            2093..3604
                     /locus_tag="VE030_00505"
     CDS             2093..3604
                     /locus_tag="VE030_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HZF21925.1"
                     /translation="MKTSATAIGLVLSFLSAAPVNLLADDRQGEGALPQHLRHLPNGL
                     PFPNPTGFASTFSTAGRIDLTNEFFQDLGANGRRCVSCHLPSAGWTIVPSQVQAIFNR
                     TLGGVIDDPLGLGAIFRTNDGSNSPAADVSTLAARRSAYSMVLTKGLIRVGIGVPATA
                     DFELVAVDDPYHFASAAELSLFRRPLPTTNLQFLSTVMWDGRETFGGTDHCNIAAEGG
                     ACFASIHFDLVDQSNGATQGHAQAPNPITNAQREAIVSFQTGLTTAQVWDEEAKALDA
                     ADAQGGPKAIFDQAFYYGINDNLGDYRTHAPFTPNVFDVYDAWTNRPGGGTNEARRAV
                     ARGQATFNSKAFTISGVAGLNFNSPFNPPLPASFQGTCTTCHNTPNSGNHSIVAPLNI
                     GLADASRRTPDMPLYTLQEKCVALSDGTKVVGPSCPVQTATDPGRALISGKFADIGKF
                     KGPILRGLAARAPYFHNGSAADLGAVVDFYNERFGAGIVGKEKEDLIAFLRAL"
BASE COUNT          793 a         1351 c         1241 g          686 t
ORIGIN      
        1 ctgcccaaca gaccgtaaaa caggctgccg atcctgcgca gcagctcatc gcccgcctcg
       61 tgaccgcagg catcgttcac gaccttgaat cggtccaggt ccagatagca cagagcgtgt
      121 tccgccatac cgacctgcgc cagcacccgc gccaggcgtt cctcgaactg cttcccgccg
      181 atcaggcccg taagggtatc gcgagcgcct tggcgggcga gttggcaggc cacccgccgt
      241 ctctcggtca catcgtgaaa aaccagcacc accccggcca tattcccgtc gcggtcgcgc
      301 aagggtgcgg ccgagtcccc gatcgcgatt tccgtcccat cggggcgcac caagagcgcg
      361 ccgtcatcca agtccacggc gcgaccttcg cggacacagc gtgcggggat gctctcgatg
      421 ggctcgcgcg tcgcgtcgga gacgagcgtg aggaccgtgg tcacgggctg cccctgcgct
      481 tcggcggcgc gccaaccggt gatccgctcc gccgtggggt tcatataaat gatcgttccc
      541 gcgacatcgg tcgtgatgag ggcgtccccc atcgacgcca ggatgacggc ggcttgctcc
      601 ttctgttgga aaaggttctc ctggacgccc ggcaacgccg tgagatcacg ccgaaattgc
      661 ttgtcctgca agggaacctc ccggcgccgc gagcggcgcc atatcactga ccgagcaaca
      721 ggaaatagca cggaaaagtg ggaaattcaa tgcgcctttc gacagggtcc cctgaacggg
      781 aaccggctgg cccgccgata ggccccgtcg cgcagtgtgc atgttgcaca agacgcccgt
      841 ggaacgctca acagcgcttc cctctgctca aaagtaagct ggcaatcatg caaatcaacg
      901 tttgcatggg ccggggcaaa gccgggaacg caatcggaag ggagaatgaa aatggcatcc
      961 aacattcgga tagcagtaga cgagacgaca cgaggcctta ccatccggtg gttcgagaca
     1021 ggactggaac gccacatctg ggtggatcag gggacgttcg agcagtttct cgcatccggc
     1081 gagacgatct atctcgacac gccggccatc gctacggaaa gacgcgcggt cgagtaggga
     1141 gaggattaaa accggggaca gccctcggtt ttcgctaaga actcccgcat gatcacacgc
     1201 taccgttagc gggtagcagt gctccgctgt attcccttcg tccgttgtga attttccaca
     1261 agaaaccggt ggaaggcttc actgcgtctc gatttgccca caactagact gctgtgacgc
     1321 gctagcagta tccagcacaa ggagaggcga cgatgtcaac gacgaattca ctgagaaatt
     1381 ccctcctacc cctgggcgtt gtcgcccttg cggccttcct ctgcgggggc ggaggcgacg
     1441 ggctggccgc gaagaccgac ggcaccaaag ccgacacggt gcggatgaag agcatcaatc
     1501 ccggggcggg cagaagcggc cccttcggcc tcaccgtctt caacaacgcg gtctacttct
     1561 ccgcctttga cggtacgagc gggtttgagc tgtggaggag cgatgggtcc gaggccggca
     1621 ccgtgcgggt gaaggacatc aatcccgggg cgaacggcag cattcctggc ggctttaccg
     1681 tcttcaacaa cgcgctctac ttctccgccg acgacggcac gagcgggttt gagctctgga
     1741 ggagcgacgg caccgaggcc ggcaccgtgc gagtcaagga catcaatccc ggggcgggcg
     1801 gcagcattcc tttcggtttc accgtcctga acaatgcgct ctacttcttc gccgacgacg
     1861 gtacgaacgg gtttgagcag tggctgagcg acggcaccga ggccggcacc atgcgggtca
     1921 aagacattct gccgcggacc gtgtggagcg gcaaccggta ggcgatcgtc tagaccaagg
     1981 gagacccgat cctgtgattg gttcacaaga atcgtgtgca acgcgacacg gaacgaagtg
     2041 tcgtcgactg ccaaactggc ctcagctttg aggcaaaaaa aaggagatga ccatgaaaac
     2101 aagcgcaacc gcaatcggtc tggttctgtc gttcctttcc gcagcaccgg tgaatttgct
     2161 ggccgacgat cgtcagggcg aaggggcgtt gccgcagcat ctgcggcacc tgcccaacgg
     2221 cctgcccttc cccaacccca ccggcttcgc ctccaccttc agcaccgccg ggcgcatcga
     2281 cttgaccaac gagttcttcc aggacctcgg tgccaacggc cgccgctgcg tgagctgcca
     2341 cctgcccagc gccgggtgga cgatcgtgcc ctcgcaagtg caggcgatct tcaaccggac
     2401 cctcggaggc gtgatcgacg acccgctcgg cctgggcgcg atcttcagga cgaacgacgg
     2461 ctcgaactca cccgcggcgg acgtctcgac cctcgcggcg cgccgcagcg cctacagcat
     2521 ggtgctcacc aaggggctca tacgcgtggg catcggggtg ccggccacgg cggacttcga
     2581 gctcgtggca gtcgacgacc cgtaccactt tgcgagcgcc gcggagctgt ccctcttccg
     2641 ccgccctctc ccgactacaa acctgcagtt cctctccacc gtcatgtggg acggtcgcga
     2701 gaccttcggc ggaaccgacc attgcaacat cgccgccgag ggcggcgcct gcttcgcctc
     2761 gattcacttc gacctggtcg accagtcgaa cggcgcgact caaggccacg cgcaggcccc
     2821 caatccgatc acgaatgcgc agcgcgaggc catcgtctcc ttccagacgg ggctcaccac
     2881 ggcgcaggtc tgggacgaag aggccaaggc tctggacgcc gccgacgccc aaggagggcc
     2941 caaggcgatc ttcgaccagg ccttctacta cggtatcaac gacaacctcg gcgactaccg
     3001 gacgcacgcc cccttcaccc cgaatgtgtt cgatgtctac gatgcctgga ccaaccgccc
     3061 cggcggcggg accaacgagg cgcggcgcgc ggtcgcacgc ggccaggcga ccttcaattc
     3121 gaaggcgttc actatctccg gcgtcgccgg gctgaacttc aacagtccct tcaacccgcc
     3181 gctgcccgcg tccttccagg gaacctgcac cacctgccac aacacgccga actcgggcaa
     3241 ccactcgatc gtggcgccgc tcaacatcgg cctcgcggat gcctcgcgcc gcactccgga
     3301 catgccgctc tacacgctgc aggaaaagtg cgtggccttg agcgacggca ccaaggtcgt
     3361 cgggccgagt tgcccggtcc agacggccac cgaccccggc cgcgcgctca tctcgggcaa
     3421 gttcgccgac atcggcaagt tcaaggggcc aatccttcgc ggcctcgccg cgcgcgcgcc
     3481 ctacttccac aacggctcgg cggccgatct gggcgcggtc gtcgatttct acaacgagcg
     3541 cttcggcgcg ggaatcgtgg gaaaggaaaa ggaagacctc atcgcgttcc ttcgcgcgct
     3601 gtaggcatga aacgatttca gaaggacttt tcgattaccg gggtggtggg tccttagccc
     3661 ttgcgggagg gctgggtatt cacgggtgtg ccaccacccc ggtaccgcac accagccgcc
     3721 atccaactct cagtcgcctc gcccgtgccg tgactagttc acaggaaacc ggtgtgatgc
     3781 gccgcagaaa aaagaaaccg ccaccgccac actggcccca gctctgaatc ctgatcgcaa
     3841 cagaagcgct tggcgcttcc ggggttcggg tgaatctcaa acgggaggcg gatatggata
     3901 ctcaaatcgg gaaggctctc aaaattgcgg cgaacatcgg gtgtgtgctg gttatgcttc
     3961 tgctgccgtt gtccgcggcc cgcggcgacg cggtatcgga ttggcgcgcg attgcagtgc
     4021 gcatgactgc atcgtcggag cagccgcgac gtgcgcagga actggcggcc g
//
LOCUS       DASQOP010000015        16483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1214233, whole genome shotgun sequence.
ACCESSION   DASQOP010000015 DASQOP010000000
VERSION     DASQOP010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 16483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16483
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214233"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            363..848
                     /locus_tag="VE030_00510"
     CDS             363..848
                     /locus_tag="VE030_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF21926.1"
                     /translation="MTDQATDLARGRALAVGTEKIFLRSAGSALCLTFIRRSEMAAKR
                     KAAKKSGKKKSTGKKKAAKKRPAAKKMAKKPAKKAKRRSGKKKKAAKKKAKKPAKKKA
                     KKARKPAKKKAKKPAKKKAKKKAGKKPAAKPAAMAAPAVPKPVSAPAAWPFPVAGNRP
                     N"
     gene            1266..1454
                     /locus_tag="VE030_00515"
     CDS             1266..1454
                     /locus_tag="VE030_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF21927.1"
                     /translation="MKAPSRNAGFICLLLFIVGVIGFFVPLGGIPYLGPVLQMLNQID
                     VYILMAGYGLLLLTVYIF"
     gene            complement(1509..2150)
                     /locus_tag="VE030_00520"
     CDS             complement(1509..2150)
                     /locus_tag="VE030_00520"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZF21928.1"
                     /translation="MAALRLGDIAPDFTQESSMGPIQFHKWIGNNWAVLFSHPKDFTP
                     VCTTELGYTAKLKAEFHKRGVKAIAVSVDPVESHIKWIDDINDTQKTKVNFPILADAD
                     RKVSNLYDMIHPNASDTFTVRSVFIIDPTKKVRATITYPASTGRNFDEILRVIDSLQL
                     TDRYSVATPVNWKDGDEVVIVPSLQDPEVIKEKFPKGYKALRPYLRMTPQPNR"
     gene            2274..2489
                     /locus_tag="VE030_00525"
     CDS             2274..2489
                     /locus_tag="VE030_00525"
                     /inference="COORDINATES: protein motif:HMM:NF025005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HZF21929.1"
                     /translation="MERKKVNSSSIRTIGYDERGRVLEVEFNDGRINQYTGVSPEVHR
                     RLMSAPSIVSYFRDNVEESFPAKRIKG"
     gene            complement(2596..3195)
                     /locus_tag="VE030_00530"
     CDS             complement(2596..3195)
                     /locus_tag="VE030_00530"
                     /inference="COORDINATES: protein motif:HMM:NF022609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HZF21930.1"
                     /translation="MDDNVLRAMAKWPNVPAVYGWLQLDRRGKWLIKGERIANPGVVA
                     FIGRNYAADEQGRWFFQNGPQRVFVTLEYAPFVIATASADAKSLIAHTGQEVERVTGA
                     WLDETGSLLLRWSGGPGLVSDRDLAGIMALFTDVHGKPVGEGTLTRAFDAEARRHATG
                     LYFDYAANRVPVGRIQAGEVPQKFGFDPDPRPKKGQPEC"
     gene            complement(3316..3489)
                     /locus_tag="VE030_00535"
     CDS             complement(3316..3489)
                     /locus_tag="VE030_00535"
                     /inference="COORDINATES: protein motif:HMM:NF022609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HZF21931.1"
                     /translation="MDPIVIQAMTKWPNVPAVYGWLQFERRGRDSLATCRPFAGTAAA
                     RRSPNRFYGGRHG"
     gene            3607..4257
                     /locus_tag="VE030_00540"
     CDS             3607..4257
                     /locus_tag="VE030_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HZF21932.1"
                     /translation="MALTFYYGSGSPYAWRVWLALEHKAIGYELKTISFSAGDLKKPE
                     FLKINPRQKVPAITDGDLALYESAAIVEYLDEQYASRKQLIPGNTKQRAVIRRLVREA
                     DEYCAHALERLVDQVLFTPQDKWNAGEIASARTALERELGIFEGYLSGDYLAGELSAA
                     DITLYPMIALCLRMEKKKPDLDVRSAIGPKLGAWMKRIEALPYFDKTYPPHWKAKP"
     gene            complement(4387..4833)
                     /locus_tag="VE030_00545"
     CDS             complement(4387..4833)
                     /locus_tag="VE030_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZF21933.1"
                     /translation="MTEVIGIDHIYISVSDLDRSEAFYDKAMAALGFRKNTFTIGGDP
                     HVQYFNRHFGFVLRPARSRIPHDSYAPGLHHFCLRVEGKEEVEDAAARLRAAGIDASE
                     PKLYPEYASDYYATFFNDPDGLRLEVTNYRAERRRRHDEWATRGQG"
     gene            4949..6214
                     /gene="otnK"
                     /locus_tag="VE030_00550"
     CDS             4949..6214
                     /gene="otnK"
                     /locus_tag="VE030_00550"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HZF21934.1"
                     /translation="MSILLGCVADDFTGATDLANTLVKAHMRTVQLLGVPRPGLKVPA
                     ADCIIVALKSRSNPANEAVTMSLAALDWLRQQGAKQFYFKYCSTFDSTDRGNIGPVAD
                     AMLDAIGADFTVFNPAFPTNKRTVYKGYLFVGDELLSESGMRHHPLTPMTDPSLVRVL
                     QRQTRHKVGLVPYATIVNGVEAIRDAFEKLRKQGIRHAILDSITDDHLVTLGEACTDL
                     KLVTGGSGMAMGLPANFVRKGLMKSGQHSQLPNVTGPTAVLAGSCSVATQGQVALMKQ
                     DHEFFELDPVAIASGRDLAQEALNWAASRLSGKPVLIYSTATPEQVKVVQERLGREHA
                     GALVEETLGKIARGLVAGGVRRLVVAGGETSGAVVGALGVEGLAIGPEIDPGVPWTFS
                     IGEPPLALALKSGNFGTPDFFTKAFKVQP"
     gene            6417..7049
                     /gene="otnC"
                     /locus_tag="VE030_00555"
     CDS             6417..7049
                     /gene="otnC"
                     /locus_tag="VE030_00555"
                     /EC_number="4.1.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate 4-phosphate decarboxylase"
                     /protein_id="HZF21935.1"
                     /translation="MNEMKLREEICILGKSMFDRGLTPGSSGNISARVDDGWLMTPTN
                     SCLGRLDPATLSKVDAKGNLISGGKPTKEAFLHLSVYERRASAGAIVHLHSTHSVAVS
                     CLSDADPQQPIPPITAYYVMKIGNLALLPYYPPGDISLANAVREVAGKHHAILLANHG
                     PVVADKDLEAAVYAAEELEETAKLYLMLRGSRLKVLSPEQVAELHRKYPS"
     gene            complement(7228..7893)
                     /locus_tag="VE030_00560"
     CDS             complement(7228..7893)
                     /locus_tag="VE030_00560"
                     /inference="COORDINATES: protein motif:HMM:NF025134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HZF21936.1"
                     /translation="MSQNFYQIGVRKLRIWPDQQALHPLDEPSIRVTRFTDIDHYHPA
                     LKRRVLELREDPQFRDYYFHGGCGWKVRWVDRWARPETELIHSRAIALFKQAYGTAAS
                     VVDDSWASLYEKGDYCMPHSHHRSIASVLYVLDPGDQDPSDRTGGKFCFADPRIAVCC
                     QVEPGRMTQLLTPTLQEGSMMVFPSEWVHLVTPYAGTRLRITLSWNLHTRLLPGRPED
                     SFK"
     gene            8177..8260
                     /locus_tag="VE030_00565"
     CDS             8177..8260
                     /locus_tag="VE030_00565"
                     /inference="COORDINATES: protein motif:HMM:NF020652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1924 domain-containing protein"
                     /protein_id="HZF21937.1"
                     /translation="MNCTLVFKRECTAKEKGDVLVWLGGIK"
     gene            8403..8879
                     /locus_tag="VE030_00570"
     CDS             8403..8879
                     /locus_tag="VE030_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014630650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HZF21938.1"
                     /translation="MTLKLTSSAFEHEGNIPRLHTCQGKDVSPPLAWSGVPENAKGLV
                     LIVDDPDAPDPAAPKMTWVHWVLYNIPRSATGLPQGVSPNELPEGTLEGISDFQRTGY
                     GGPCPPIGRHRYFHKLYALDTVLPNLKKPRKADVEKAMKGHVIEQAVLVGTYQKSQ"
     gene            9036..9623
                     /locus_tag="VE030_00575"
     CDS             9036..9623
                     /locus_tag="VE030_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF21939.1"
                     /translation="MLLRIQSGSLLTQLVVVLLCDIQLAAAESPAPFEYKGLLGHMTI
                     EQAQDYDGHSLTCETDVVVPALVHCHDHSTTYQGKPAILKLEFIDGNLAMLNLLIPAE
                     HFGEISRSIVGRYGKPRRHGTETDGPKPYEHMEWALADGELILDEWIASFNPADQRFA
                     TVLGLKFLPTALQKRRAEVAWTRHRAIEKRKQADM"
     gene            complement(9728..9955)
                     /locus_tag="VE030_00580"
     CDS             complement(9728..9955)
                     /locus_tag="VE030_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF21940.1"
                     /translation="MQKQTVPTARYVELLNEEIKRHPYYQKGMQFVAAPSPYTAPQLL
                     DVTWDKEKWSEYNFVFAAAHRVVRARYHAKS"
     gene            complement(10293..10580)
                     /locus_tag="VE030_00585"
     CDS             complement(10293..10580)
                     /locus_tag="VE030_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF21941.1"
                     /translation="MKALFLVVICCLAFVAYAEEQAPSTADRVKSDAKGAARGVAKAA
                     KDVGKQIGAGTKNAVKSIKTKVKTDVNQGTPGDGSAKRQNEKMDTAKAGRK"
     gene            complement(10779..12473)
                     /locus_tag="VE030_00590"
     CDS             complement(10779..12473)
                     /locus_tag="VE030_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HZF21942.1"
                     /translation="MTSIRFDTYYRYADLVRFLTGWAETHPDLVRLEPVGKSYEGREI
                     YVVRVTSYKTGLDVDKPAFWVDGNIHATELAGSAACLHLIQRLLTGYGRDPEITRCLD
                     TRAFYVCPRVNPDGAEWALADLPKLIRSSTRPYPYDEEPVGGLIREDADGDGRILSMR
                     VPDPNGPWKLCSEDPRILVRRDPTESGGQYYRLLPEGRLEDYDGVTIRLQARRERLDL
                     NRNFPAYWRTENEQIGAGPFPTSEPEIAAITGFIARHPNITGACAFHTYSGVLLRPYS
                     HLADEQMPPEDLWTFQKIGAKGTEITGYPHASAYHEFRYHPKEVITGDFDSWMYDHRG
                     VYAWTVEIWSPQRQAGIGDYKYIEWYREHPLEDDLKLMKWNDTVLKGKGFVNWYAFDH
                     PQLGRVELGGWNSLYTWTNPPHHLLEKEIAPFSRWLVWQALISPRLELLRASVTALGK
                     NAWRIMLVVQNSGWLPTYVTRMAKQKKLARGVVCEIELPEGATLETGKLREELNQIEG
                     RAHKPAAANTWAGSSADETDDRLKVEWVVRAPKGAMLKLTARHERAGTVRTELRLE"
     gene            complement(12731..13426)
                     /locus_tag="VE030_00595"
     CDS             complement(12731..13426)
                     /locus_tag="VE030_00595"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZF21943.1"
                     /translation="MYSSKVIADFGVNDLKLKKWAIVHSTDAFGNGGMNALTAELKKF
                     GIEPVLVQGFTNNTVDFTPIVLSLKRTNPEALGTYIPFSTDQGIFAKQLQQLGVRVRW
                     VGSPTTVSETAMKLAGSALHGSYAVTDFHAGANDEARAYSRAYRERYRIDADNYSAWT
                     YDALHLLALAINNAGSTDPEAVREAIVGIRGYIGAEGVYNFDESGEGVRGYNVVRNDN
                     GTVVFIKRVDFDR"
     gene            complement(13408..13866)
                     /locus_tag="VE030_00600"
     CDS             complement(13408..13866)
                     /locus_tag="VE030_00600"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZF21944.1"
                     /translation="MGNGMLRALHCLLYFAAAFPLGVHAAGTIKIGLVAEITGQAAEA
                     GMYTVNGARLAIEDINKAGGVLGVPVELHVEDSQGSNPGAVLAFSKLFSRGDIPAIVG
                     PIRSTQIQAASPSIARAGIPVMIGGTDPSLTRVNNRWYSAAGPMICIRPR"
     gene            complement(13866..14852)
                     /locus_tag="VE030_00605"
     CDS             complement(13866..14852)
                     /locus_tag="VE030_00605"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZF21945.1"
                     /translation="MEYVSLGKTGLKVSRICLGCMSYGSPKWRDWVLDYDQSKPFFKR
                     ALELGINFFDTADMYSDGESEVVTGRALKEFGPSRDKLVIATKVFNPMGKDPNQRGLS
                     RKHIMHAIDDSLRRLGLDYVDLYQIHRFDRHTPIEETLEALTDVVKAGKALHIGASSM
                     YAWQFAKMLYSADANGFSRFVSMQNHYNLVYREEEREMIPLCIDQGIGIIPWSPLARG
                     FLAGNRSKQDWGATTRAKSDDFAHDMYYQDSDFEVVNRVGEVAKRRGVPNAQVALAWL
                     LQQPGVTAPIVGATRMQHLEDALAALALKLTGEELKALAEPYKPHPVLGNLR"
     gene            complement(14887..15888)
                     /gene="dusA"
                     /locus_tag="VE030_00610"
     CDS             complement(14887..15888)
                     /gene="dusA"
                     /locus_tag="VE030_00610"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HZF21946.1"
                     /translation="MPQSTPHSHRLCTAPMMDWSDRHCRFFFRQLSPHAVVYTEMVTT
                     GALIHGDVERHLRFHPAEHPVALQLGGSEPVDLARCATLGLAYGYDEVNLNCGCPSER
                     VQHGAFGACLMAEPQLVADCVKAMQDAVAVPVTVKHRLGIDSIEDYDFVRRFVETVAA
                     ADCRTFFVHSRNAILKGLSPKDNRAIPPLKYDYVYRLKREFPELGIVINGGVEALRAI
                     EQHLQHVDGVMLGRAAYHDPYVLAAAEAMLSSEPLPSRAQIVQSMFEYASEEVARGTS
                     LRAIARHMLGLYHGQPNARLWRRMLSDAGMLAANRTELLLNAVLAVESAEAEIAAVA"
     gene            complement(15929..16393)
                     /locus_tag="VE030_00615"
     CDS             complement(15929..16393)
                     /locus_tag="VE030_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF21947.1"
                     /translation="MTRLAALLSLGAFVAGNAVPVEQGLGPHEVGVSAPAQITYSLDA
                     RPKPERLDFDFRAGDSHAWINDGGDFQVSGWIKHTGLLCGTYRMGLRFGTGSPGCLNV
                     EWITEPLFVTVEIQCNDARIEHSGGDSDSGLGKRLATITCGERVIRCTGNCK"
BASE COUNT         3286 a         4854 c         5074 g         3269 t
ORIGIN      
        1 gagacccgtg tgatcgagta tcagaccggc ggggctctga actgggattg atgcatgggc
       61 cggaaagttt tctgtgacac ccgtgctgcc gccggtcgat tcaacggccc ggcagcgcac
      121 ttgttagggc tgaatgggta aaccggacaa gcagttcact gatcaatcac gaaacgcgcg
      181 gtgcctctcc aaagagcaag ccgccaggct gggagcaatg agcccccagt cgaaggtttc
      241 ctttccgctt catttccggc aattggccga tgcgggtgtt attccctttc tggccgcccc
      301 ccctctcacg aatcgcgctg cggacacggc acgtcttcgt ccgcggccaa cagaactgtc
      361 caatgaccga tcaagcgacc gatttggcgc gcgggcgggc attggcggtt ggcaccgaga
      421 agatttttct tcgaagtgct ggtagtgccc tttgtttaac tttcattagg agatcagaaa
      481 tggcagcgaa gagaaaagca gcgaagaaat ccggcaagaa gaagtccacg ggcaagaaga
      541 aggccgccaa gaagcgtcct gcagccaaga agatggcgaa aaagccggca aaaaaggcaa
      601 agcgccgttc cggcaagaaa aagaaagcgg cgaagaaaaa ggccaagaag ccggccaaaa
      661 agaaggcgaa gaaggccaga aagcctgcga agaagaaggc caagaagccc gcgaaaaaga
      721 aagcgaagaa gaaagcgggc aagaagcctg cggccaagcc agctgccatg gcagctccgg
      781 ccgtgccgaa gccagtttct gcaccggcgg cttggccgtt tccggtagcg ggtaaccggc
      841 ctaactaagc ttcccagatg ctcgcgggca gtctgcatcc gcgatggata ccagacactg
      901 agccgcgagc tttggacttg cagtaaacat aaccgggccc gcacgggcgc ggtcgagttg
      961 gagctttaag caaacctccg ccgatcaaga gacgggaggc gggtaagttc ctaacgcaaa
     1021 tcttcttgtc ccgttgcggg acgagagggt ggtttttttc ctactgaaag tgaaacgcta
     1081 agcacttcaa agatcaagta aaaatcccgg cgggcgttgg cccggaaagg gcacccggga
     1141 accgtcggga cgaagcgaac ccagtcgggg aattcatggg acgaggcggc ggatcgaatc
     1201 tgctgcctct ttgatttcag tccgcggcgg ttgtagggcg gccgctgaag aggaggaaaa
     1261 taacgatgaa agcaccaagc agaaacgcag gcttcatctg cctgctgctg tttattgtcg
     1321 gagtgatcgg ttttttcgtg ccgcttggcg gcatacctta cctcggcccg gttttgcaga
     1381 tgttgaacca gatcgacgtt tacattctga tggctggtta cggcttgttg ctcctgaccg
     1441 tctatatttt ctaggtaaaa aagcggctgg tctccaagac agcccccagg cctcagctga
     1501 ctgctcgatc agcgattggg ttgcggcgtc atgcgcaggt agggacgcag cgccttgtag
     1561 cctttcggga acttctcctt gattacttcc ggatcctgca acgacggcac gattaccacc
     1621 tcgtcgccgt ccttccagtt caccggggtg gcgacgctgt accggtcggt gagttgcagc
     1681 gaatcgatta cccgcaggat ctcgtcgaaa ttcctcccgg tgcttgccgg gtaggtgatt
     1741 gtcgcgcgca ccttcttggt cgggtcgatg atgaaaaccg agcgcacggt gaatgtatcg
     1801 ctggcattcg ggtggatcat gtcatagagg ttggacacct tccggtccgc gtccgccagg
     1861 atcgggaagt tgaccttggt tttctgcgtg tcgttgatgt cgtcgatcca cttgatatgg
     1921 gactccaccg ggtcaacgct cacagcaatt gccttgacgc cgcgcttgtg gaattccgcc
     1981 ttcagcttgg ccgtgtagcc gagttcggtg gtgcacaccg gggtaaaatc cttcgggtgg
     2041 gagaacagga cggcccagtt gttgccgatc cacttgtgaa attggatcgg tcccatgctg
     2101 gactcctgcg tgaaatcggg ggcaatgtct cccagtcgta atgcagccat gatggtctcc
     2161 ggtaagcgtt cgaaacttag caagtgagtc tagcggcgcc ctatatattc cacaaacgaa
     2221 cactttcaca tatcttaatt ccggatgggc ttagtgttag tcggagtgaa cccatggaaa
     2281 gaaaaaaagt gaattcgagc tcaatccgga cgatcgggta cgacgagcgc ggccgcgtgc
     2341 tggaggtgga gttcaacgac ggccgcatca atcagtacac cggggtctcg ccagaagtgc
     2401 accggcggct gatgagtgca ccgtcgatcg tcagctattt ccgcgataac gtcgaggagt
     2461 ccttccccgc gaagcgaatt aagggctgaa tcccgtgccg agtactgggt gcggcgtgct
     2521 gagaaaaggc tggaatagcc tggccacctt aagtgcgaag catcccgctt cttcgcagcc
     2581 ctcagtacgc tgcattcagc actctggttg tcctttcttc ggccggggat ccggatcgaa
     2641 gccgaatttt tgcggtacct cgccggcctg aatgcgtcct accggcacgc ggttggcggc
     2701 atagtcgaag tacagaccgg tcgcgtgacg tcgggcttcg gcatcgaaag cccttgtcag
     2761 cgtgccctcg ccgaccggct taccgtgaac gtcagtgaac aaggccatga tgcccgccag
     2821 gtcgcgatcg ctgaccagtc ccggcccgcc gctccagcgc aacaacagcg agcctgtttc
     2881 gtccagccac gctccggtaa cccgctccac ttcctgtccc gtatgcgcaa tcagcgactt
     2941 ggcgtcggcg ctggcggtgg caatcacgaa aggcgcatat tccagtgtca cgaacacgcg
     3001 ctggggtccg ttctggaaga accagcgccc ctgttcgtcg gcggcgtaat tgcggccgat
     3061 aaaggccacg acgccggggt tggcgatgcg ctcacccttg atcagccact tgccgcgccg
     3121 gtcgagttgg agccagccgt agaccgccgg tacgttgggc catttagcca tggcgcggag
     3181 aacattatcg tccatggttt tcaacgaaag gtcgaggcgg cggtcatgcg ccgcttcccg
     3241 caagcgggcc tacggctaac gtgtccgcgg cgcgctggaa cattcggcca cttggctatg
     3301 gatcgcagta ctgcatcatc catgccgacc tccgtagaag cgattggggg accggcgcgc
     3361 ggcggccgtt cccgcaaacg ggcgacaagt cgctaacgag tcccggccgc gccgctcgaa
     3421 ttggagccag ccgtagaccg ccggtacgtt gggccatttg gtcattgcct gaatgacgat
     3481 cgggtccatg cggatgcaga ataggcgggt gcgaaaaaat aaaattctac ccgtcgggca
     3541 caacgatggc ccgcggagac atgatcccag tagacttgtc accagtcacc aatcacggag
     3601 tcgtcaatgg cactgacctt ctactatggt tccggctcgc cttatgcttg gcgggtctgg
     3661 cttgcactcg aacacaaggc gattggctac gaattgaaga cgatctcgtt ctcggcgggc
     3721 gacctcaaga agccagagtt cctgaagata aacccgcgcc aaaaggtacc ggccatcacc
     3781 gatggcgacc tggccctgta tgaatcggcc gcaatcgtgg aatacctcga cgagcaatac
     3841 gcttccagaa agcagctcat tcccggcaat accaaacagc gcgcggtgat ccggcgcctg
     3901 gtgcgtgaag cggacgagta ttgcgcccat gcgctggagc gcctggtcga ccaggtactg
     3961 ttcacgccgc aggacaaatg gaatgccggt gagatcgctt ccgcccgcac ggccctggaa
     4021 agagaactcg gaattttcga gggatacctg tccggtgatt atctggccgg tgaactttcc
     4081 gctgcggaca tcacccttta tccgatgatc gcgttgtgtc tgcgcatgga gaagaagaag
     4141 ccggatctcg atgtgcggag cgcgatcggg ccgaagctcg gcgcatggat gaagcgcatc
     4201 gaagcgctgc cgtacttcga caagacctat cccccgcact ggaaagcgaa gccatgaagg
     4261 caggggttag ggatagacca gggactaggg gttagggatg atcagggact aggggctagg
     4321 ggaagagcag ggggttaaag tttcaggcca agagttttcc ctagcccctc gtccctcgct
     4381 cctgctctat ccctggcccc tcgtggccca ctcgtcgtga cggcggcggc gttcggcgcg
     4441 atagttggtc acttccagcc gcaagccatc cgggtcgttg aaaaaggtcg cgtagtaatc
     4501 cgacgcgtat tccgggtaga gcttcggttc cgacgcatcg atacccgcag cccgcaatct
     4561 cgccgctgcg tcttccacct cttctttccc ttctacgcgt agacagaagt gatggagtcc
     4621 cggcgcatac gagtcatgag gaatccggct tctggccggc cgcagcacga aaccgaagtg
     4681 ccggttgaag tattgaacgt gcgggtcgcc accgatagtg aacgtgttct tcctgaagcc
     4741 gagggcggcc attgccttgt catagaacgc ctcggaacgg tcgaggtcgc tcacggagat
     4801 atagatgtgg tctattccga tgacttcggt catgggtgcg aagactccgg actttcgatg
     4861 ccgggtttcg gtaccattat gctcaaatac caacacatat cagggaggaa gtcctcgtgc
     4921 tcgaattcat ttccccggga gacgcgccat gagcatcctg ctcggctgcg tcgctgacga
     4981 cttcaccggc gcaactgatc tcgccaacac gctggtcaag gcccacatgc gcaccgtgca
     5041 actgctgggc gtgccgcgtc cggggctgaa agtgcccgct gcagactgca ttatcgtggc
     5101 gctcaagtcg cgcagcaatc ctgccaacga ggcggtcacc atgtcgctcg ctgcgctcga
     5161 ctggttacgg caacagggcg cgaagcagtt ctacttcaaa tactgttcga ccttcgattc
     5221 caccgaccgg ggcaacatcg gcccggtcgc cgacgcgatg ctcgatgcaa tcggcgcgga
     5281 cttcaccgtg ttcaacccgg cgtttccgac caataagcgc accgtctata aggggtatct
     5341 gttcgttggc gacgaactgc tatccgaatc cgggatgcgt caccatccgc tcaccccgat
     5401 gaccgatcct tcgctggtcc gagtgctgca gcggcagacc aggcacaaag ttggattggt
     5461 gccgtacgcg accatcgtga acggagtcga ggcaatccgc gacgccttcg aaaagctccg
     5521 caagcaggga atccgccatg cgatcctcga ttcgatcacc gacgatcatc tggtaacgct
     5581 gggcgaggcc tgcaccgatc tgaagctggt caccggcggc tccggaatgg cgatgggcct
     5641 gcccgcgaac ttcgtgcgca agggcctgat gaaaagcggg cagcactccc agttgccgaa
     5701 tgtcaccggc cccaccgccg tgctggcggg atcctgctcg gtcgcgaccc agggacaggt
     5761 cgcactgatg aagcaagatc acgagttctt cgaactcgat cccgtggcga ttgcttccgg
     5821 ccgcgatctc gcgcaggaag cgctcaactg ggccgcgagc cggctctccg gaaagccggt
     5881 cctgatctat tcgaccgcca ctccggagca ggtgaaggtg gtacaggagc gcctgggccg
     5941 cgagcatgcc ggcgcgttgg tggaagaaac cctgggcaag atcgccaggg ggctggtggc
     6001 gggtggcgtg cgacggctgg tggttgcggg tggcgagacc tccggcgcgg tggtcggtgc
     6061 gctcggcgtc gagggtctcg caatcggccc ggagatcgac cccggggtgc cctggacgtt
     6121 ttctatcggc gaaccaccgt tggcgctggc gttgaagtcc gggaacttcg gcactccgga
     6181 tttcttcacc aaagcattca aggtccaacc gtgactcggg caaccttcac cgcaaagacg
     6241 caaaggacgc agaggaaacc aattgagggt aattggcaat ctatgagcac cactcagcct
     6301 gtctgcaaat atcaatgatt tttgcgccat tctcgatttt tcttttttgt cctggacttt
     6361 ccttcgcgtc ctttgcgact ttgcggtgag agtgggttct tagacagaaa atagacatga
     6421 acgaaatgaa actcagggaa gagatctgca tactcggcaa gtccatgttc gatcgcgggc
     6481 tcacgcccgg aagttccggc aacatcagcg cccgggtgga cgacggctgg ctgatgacgc
     6541 cgaccaactc ctgcctggga cggctcgatc cggcaaccct ttccaaagtc gacgcgaagg
     6601 gcaatcttat atccggtggc aagccgacca aggaagcctt tctgcatctt tcggtgtacg
     6661 agcggcgggc gtccgccggt gccatcgtgc acctgcattc cactcattcg gtggcggtgt
     6721 cctgcctctc cgacgccgat ccgcaacaac cgattccgcc gattacagcg tattacgtga
     6781 tgaagatcgg gaacctcgcg ctgttgcctt attatccgcc gggcgacatt tcactcgcca
     6841 atgcggtgcg cgaagtcgcc ggcaagcatc acgcgatcct gctcgccaac cacggcccgg
     6901 tggtggccga caaggacctc gaggcagcgg tttatgctgc ggaggagctg gaggaaacgg
     6961 cgaaactgta tctgatgttg cgaggcagtc ggctcaaggt tctgtctccc gaacaggtgg
     7021 cggaattgca ccggaagtac ccgtcctgaa gtgtggtatg gggtgacgcg aagtagataa
     7081 ttgtcatgct cgcggttgta caggatcggc caggtgaagg ctgatgtcga gagcatcgac
     7141 acaaaaggcg caacgaaaaa agggaggaaa caaaggcgaa agtaaaggca agctggagtg
     7201 gtttcttcaa gttacccgtc atccgtttta cttgaaagaa tcctcaggcc tgccggggag
     7261 caagcgggta tgtaaattcc aggacaaagt gatccgcagt cgggttccgg cgtacggcgt
     7321 cacaagatgc acccattcgc tgggaaacac catcatcgag ccttcttgca gcgtcggggt
     7381 cagcaactga gtcattcgcc caggttccac ctgacagcag actgcgatgc gagggtcagc
     7441 gaagcagaat ttgccaccgg tacggtcgga cggatcttgg tctccgggat cgagtacgta
     7501 caagacgctg gcgatcgacc gatgatggct gtgaggcatg cagtaatcgc ctttctcgta
     7561 aaggcttgcc caactgtcgt cgactaccga ggcggcggtc ccgtatgcct gcttgaacaa
     7621 agcgatcgcc cgggaatgaa tgagttcggt ttcgggccgg gcccagcggt cgacccatcg
     7681 gactttccat ccgcagccgc cgtggaagta gtaatcacgg aattgcgggt cttcgcgcaa
     7741 ctcgagaaca cggcgcttga gcgcggggtg gtaatgatcg atgtcggtaa agcgggtaac
     7801 ccgaatcgac ggctcatcaa gcggatgcag cgcctgctga tccggccaga tgcgaagctt
     7861 gcgtacgccg atctgataga aattctgcga catgacagga aattaggcac cgggacaatg
     7921 acatcgtcaa gcccgccgcc ggccggaagt caacttcaag cgtattcaac gcgaagggca
     7981 caagaaatgc gcgcgggaat ggcgcgatta caaaccgtga cgtgccggcc acagaggaac
     8041 acggagaagg actatacttt ttagggtcac ccggttacag gtgctgccat gggaaaccgc
     8101 tacggcacgt tcaaggactt ttacccgttc taccttagcg tgcaccgacc cacggactgt
     8161 ggaagacttc ctgcgggtga attgcacgct ggtattcaag cgcgaatgca cggcgaaaga
     8221 gaagggcgat gtgctggtgt ggttgggcgg gatcaagtga tgtgaccgtg gtttcgggcg
     8281 tgcacgcggc aaatgagctg ccggggccgc tgatgcggcg cggtttgccg tggattgcgt
     8341 ttgccgtgct ctttttatcc gcagcagcca ctcagagtgt ccatgccaag gaggaacaat
     8401 tcatgacgct caagctgaca tcatccgcct tcgagcatga aggcaatatt ccaaggctcc
     8461 atacctgcca gggaaaggac gtctctccgc cgctcgcgtg gagcggcgtt ccggaaaacg
     8521 caaagggcct ggtgcttatc gtcgatgacc ccgacgcgcc cgatccggcc gcgccgaaga
     8581 tgacctgggt ccactgggtg ctctacaaca tcccgcgctc ggcgacgggc ctgccgcaag
     8641 gcgtctcgcc aaacgagctg cccgaaggaa cgctggaagg cattagcgat tttcagcgca
     8701 ccggctacgg cggcccgtgt ccaccgatcg ggcgccatcg ctatttccac aagctttacg
     8761 cgctcgatac cgttcttccg aatttgaaga aaccgcgcaa ggccgatgtc gagaaggcga
     8821 tgaagggcca tgtcatcgag caggcggtgt tggtggggac ttatcagaag agtcagtaag
     8881 gcgggcaatt gcgccgttgt ccaatttttt gtgtctcggg ctgcgaaatg atcgagaagt
     8941 gttggagcaa agtggcggtt tccccggcga gcaaacgcgc atgaacggaa atatgtaatc
     9001 ttcagcttca agagaaacca gggaaaagta caagcatgtt attgcgcatc caatcaggtt
     9061 ctctgctgac ccaattggtc gtcgttctcc tgtgcgacat tcaacttgcc gccgccgaat
     9121 ccccagcgcc attcgagtac aagggcctgc ttggtcacat gaccatcgag caggcgcagg
     9181 actacgacgg gcattcgctc acctgcgaaa ccgacgtagt cgtgccggcg ttggtccact
     9241 gtcacgatca ctcgaccact taccagggaa agcccgcgat tctcaagctc gagttcatcg
     9301 acggcaacct ggcgatgctg aacctgctga taccggcgga acacttcggg gaaatcagcc
     9361 gcagtatcgt cggccggtac ggcaagccac gccggcacgg gaccgaaacc gacggaccga
     9421 aaccctacga gcacatggaa tgggcgctcg ccgatggcga gctcatcctc gatgaatgga
     9481 tcgccagttt caacccggcc gatcagcggt ttgctaccgt tctgggattg aagttcctgc
     9541 cgacggcgct gcaaaagcgg cgtgccgaag tggcctggac acggcacagg gcaatcgaga
     9601 agcgaaagca ggccgacatg tagcaggata aagccgggaa actgacccgg ctcgacgcgc
     9661 gcactggcgc gcaggagctt gcaggagcgt gcggactgga ggagtccgcg cgcagatcat
     9721 cagactgcta tgacttggcg tggtacctcg cccgcactac acgatgggcg gctgcgaaaa
     9781 cgaagttgta ctcgctccat ttttccttgt cccacgtgac atccaggagc tggggggcgg
     9841 tatagggaga cggcgccgca acaaactgca tgcctttctg gtagtaaggg tgccgtttga
     9901 tttcctcgtt gagcagctca acgtagcgcg ccgtgggaac agtctgcttc tgcatgtctt
     9961 gcctccaaaa gaacccgaat tgctctatag cgaactcgtc acgggcgcaa atgctgccgg
    10021 cctgtggaaa ccgcataccg gatgtctaac atcggaatcg gctgagttgg gtgttgcatg
    10081 atcagggacg cgtggcccct gaatttcggc gggagtccgc ggacttgctc gccgcccgac
    10141 agcgcccgca ccattggtgc ggaatggcga cgctagttga ttgtattact caagcacctc
    10201 attgacaagg aaaaaacgca atcgggccgg agttaccgcg ggtttatcgg cgagtatcgc
    10261 cacccgggaa cgaccgccgg agtgcctaag gctcacttcc tgcctgcttt cgcggtgtcc
    10321 atcttttcgt tctggcgttt tgcgctgccg tctccgggcg tgccctgatt gacgtcggtt
    10381 tttacttttg tcttgatgct cttgacggca ttcttggtgc ccgcgccgat ctgtttgccc
    10441 acgtcctttg ccgctttcgc tacgcctctg gcggcaccct tggcatcgga cttcacccgg
    10501 tcggcggtcg agggagcttg ctcttcggcg taagcgacga acgcaaggca gcaaatgaca
    10561 acaagaaata gcgccttcat tgcggttcac ctcataatct tttggcgaca gggcgtcagt
    10621 ttcagagatc gggtggcaag tactgactcc gggaagcggg tcccagcctc tgcgaatagc
    10681 accgtgctgc ctggcttaac gttccaggat cgtggtgcgc cgacaaaatt tcccgcattc
    10741 cgaacaggcc ggatccaatc cggctacccc gcagtgggtc actccagacg cagttccgtg
    10801 cgcaccgtgc cggcgcgttc gtgccgcgcg gtgagcttta gcatcgcgcc tttgggcgcg
    10861 cgcaccaccc actccacttt aagcctgtcg tcggtttcgt ccgcgctcga ccccgcccag
    10921 gtgttcgcgg cggcgggctt gtgggcacgg ccttcgatct ggttgagttc ctcgcgcagc
    10981 ttgccggttt ccagcgtcgc gccttccggc agttcaattt cgcacaccac accgcgtgca
    11041 agcttcttct gtttcgccat cctggtcacg taagtcggaa gccagccgct gttctgcacc
    11101 accagcatga tgcgccaggc attcttaccc agcgccgtca cggaggcccg gagaagttcc
    11161 agccgcggcg agatgagcgc ctgccacacc agccagcgcg agaacggcgc gatttccttt
    11221 tccagcagat gatgcggcgg attggtccag gtgtagagac tgttccagcc gccaagctcg
    11281 acgcgcccga gttgcggatg gtcgaatgca taccagttca cgaagccctt gcccttcaga
    11341 actgtgtcat tccatttcat cagcttcaga tcgtcttcca gcggatgctc gcggtaccac
    11401 tcgatgtact tgtaatcgcc gatcccggcc tggcgctgtg ggctccagat ttccacggtc
    11461 caggcgtaca cgccgcggtg gtcgtacatc caggaatcga aatcgccggt gatgacttcc
    11521 ttcgggtgat agcggaattc gtgatacgcc gaggcgtgcg gatagccggt gatttccgtg
    11581 cccttcgcgc cgatcttctg gaaagtccac aggtcttccg gtggcatctg ttcatctgcc
    11641 aggtgtgaat acgggcgcag caacacgccg ctgtaagtgt ggaacgcgca cgcgccggtg
    11701 atgttcgggt gccgggcgat gaagcctgta atcgcggcga tctccggttc ggaggtcggg
    11761 aaggggccgg cgccgatctg ttcgttctcg gtgcgccagt acgccggaaa attgcgattg
    11821 agatccagcc gctccctgcg cgcctgcagc ctgatggtga cgccgtcgta gtcttcgagc
    11881 cgtccctcgg gcagcaagcg gtagtactgc ccgccgcttt cagtcgggtc gcggcgtacc
    11941 aggatgcgcg ggtcctccga acaaagtttc cacggtccgt tggggtcggg aacgcgcatc
    12001 gacaggatgc gcccgtcgcc gtccgcgtcc tcccggatca atccgccgac cggttcctcg
    12061 tcgtagggat aaggccgggt gctggagcga atgagttttg gcaggtcagc gagcgcccat
    12121 tccgcgccgt cgggattgac tcgcggacag acgtagaagg cgcgggtgtc gaggcagcgg
    12181 gtgatctcgg gatcgcggcc atagccggta agcaggcgct ggatcaggtg caggcaggcg
    12241 gcggaacccg ccagttcggt ggcgtggatg ttgccgtcca cccagaaggc gggcttgtcc
    12301 acgtccagcc ctgtcttgta gctcgtgacg cgcaccacgt agatctcgcg cccctcgtag
    12361 ctctttccga ccggctcgag ccgcaccaga tccggatgcg tctcggccca accggtgagg
    12421 aagcgcacca ggtcggcgta gcggtaatag gtgtcgaagc ggatcgaggt catgtgacgc
    12481 ggccgggtat cgcggcatcg cgctaggaga tatcagtttt cggcacaggt tgtccacctc
    12541 gggaaaaaga acacggggcg gcccgcagcg gcgacgtcgg atcgccaacg catttggcgg
    12601 attcaaaggc agccccgttc gcatcggccg cggtgcggca gtctcgaagt atgtgcccgt
    12661 cgcgtacgcg agccaagcgg cgccgcacca atccgccagc gggctccagc gaccccggat
    12721 ttacccccgg ctaccggtcg aagtcgacgc gtttgatgaa aacgactgtg ccattgtcgt
    12781 tcctcaccac gttgtagccg cgcacgcctt ctccgctttc gtcgaagttg taaacgcctt
    12841 ctgcgccgat atatccgcgg ataccgacaa tggcctcgcg cacggcctcc gggtctgtgc
    12901 ttcctgcgtt attgattgca agcgccagaa ggtgaagcgc gtcgtaggtc cacgccgagt
    12961 aattgtcggc atcgatcctg tagcgctccc ggtacgccct ggaataggcc ctcgcttcgt
    13021 cgttcgcgcc tgcatggaaa tcggtgaccg cataactgcc gtgcaacgcg gatccggcca
    13081 gcttcatcgc cgtttccgac accgtcgtcg gcgagccgac ccacctgacc cgaaccccga
    13141 gctgttgcag ttgtttggcg aagatcccct ggtccgtgga gaaagggatg taagtgccga
    13201 gggcttcggg gttggtgcgt ttgagggaaa gaacgatcgg cgtgaaatcg acggtgttgt
    13261 tggtgaagcc ctgcaccagc accggctcga ttccgaattt cttcagctcc gcggtcagcg
    13321 cattcatccc gccgtttcca aatgcgtcgg tggaatgcac gatcgcccac ttcttgagct
    13381 tcaggtcgtt cacgccgaag tcggcgatca ccttggacga atacaaatca ttgggccggc
    13441 agcggaatac cagcggttat tcacgcgggt aaggctcggg tcggtgccgc cgatcattac
    13501 cggaataccg gccctggcga ttgaaggcga ggcggcctga atctgggtgc tgcggatcgg
    13561 cccgactata gccgggatgt ccccgcgcga aaacagcttg ctgaacgcaa gcaccgcgcc
    13621 cggattactg ccttggctgt cttccacgtg cagctctacc ggcactccga gaacgccgcc
    13681 tgccttgttg atgtcctcga tcgccagcct ggcgccgttg accgtgtaca tgccggcttc
    13741 ggcggcctgc ccggtgatct ctgccaccag gccgatcttg atcgtgccgg cggcgtgaac
    13801 gcctagcgga aatgcggcgg caaaatacag aaggcaatgc aatgcacgca gcatgccgtt
    13861 acccatcagc gaaggtttcc caggaccggg tgcggtttgt agggttcggc cagcgccttg
    13921 agttcttccc cggtcagttt cagcgccaga gccgcgagcg cgtcctccag gtgctgcatc
    13981 ctggtcgcgc cgacgatggg cgcggtgacg ccgggttgct gcagcagcca cgccagcgcc
    14041 acttgcgcat tcggcacgcc gcgcctcttc gctacttcac cgacccggtt cacaacttcg
    14101 aagtccgaat cctggtagta catgtcgtgg gcgaagtcgt cgctcttggc gcgcgtggtc
    14161 gcgccccagt cctgcttgct gcggtttccg gcaaggaagc cgcgggccag cgggctccac
    14221 ggaatgatgc cgattccctg gtcgatgcag agcgggatca tctcgcgctc ctcctcgcga
    14281 tacaccaggt tgtagtggtt ctgcatcgaa acgaagcgcg agaacccgtt cgcgtccgcg
    14341 ctgtagagca tcttggcgaa ctgccaggcg tacatggaag aggcgccgat gtggagcgcc
    14401 ttgccggcct tgaccacgtc ggtcagcgct tccagcgttt cctcgatcgg cgtatggcgg
    14461 tcgaagcggt ggatctggta gaggtcaacg tagtccagtc ccaggcgccg caggctgtcg
    14521 tcgatcgcgt gcatgatgtg cttgcgcgac agcccgcgct ggttcggatc cttgcccatc
    14581 gggttgaaca ccttggtggc gatcaccagc ttgtcccggg atgggccgaa ctccttgagc
    14641 gcacggccgg tcacgacctc gctttcgccg tcggagtaca tgtccgcggt gtcgaagaaa
    14701 ttgatgccga gttccagcgc gcgcttgaag aacggcttcg actgatcgta atccagcacc
    14761 cagtcgcgcc acttgggcga gccgtagctc atgcagccga ggcagatgcg cgagactttc
    14821 aggccggtct tgccgagact gacgtattcc attggagagt tttgcctttc gaggactttg
    14881 cgacgtttac gcaaccgctg cgatctcggc ctctgcgctt tcaaccgcga gcacggcatt
    14941 tagcagcaat tcggtgcgat tggcggcgag catgccggca tccgacaaca tgcgccgcca
    15001 cagcctggcg ttgggttgac cgtgatagag gccgagcatg tggcgggcga tggcgcgcaa
    15061 cgaagtgccg cgcgcaacct cttcggaagc gtactcgaac atcgattgca cgatctgcgc
    15121 gcgcgaaggc agcggttcgc tggacagcat tgcctccgcg gcggcaagca cataaggatc
    15181 gtgataggcc gcgcgcccga gcatgacgcc atccacgtgc tggaggtgtt gctcaatcgc
    15241 ccgcagcgct tccacgccgc cgttgatgac gatcccaagt tcggggaatt cccgcttcaa
    15301 ccgataaacg tagtcgtatt tgagcggcgg gatcgcgcgg ttgtccttcg gcgacaggcc
    15361 cttcaggatg gcattccggg aatggacgaa aaaagtcctg cagtccgcgg ccgcgacggt
    15421 ttcgacaaag cggcgcacga agtcgtagtc ctcgatcgag tcgataccca gtcggtgctt
    15481 caccgtgacc ggaacggcta cggcgtcctg catcgccttc acgcaatcgg ccaccagttg
    15541 cggttcggcc atcagacagg ccccgaatgc gccgtgctgc acgcgctccg aggggcagcc
    15601 gcagttcaaa ttgacctcgt cgtagccgta cgccaggccg agtgtcgcgc aacgggcgag
    15661 atccacgggc tcgctgccgc cgagctgtag cgccaccgga tgttcggcgg gatggaagcg
    15721 caggtgccgc tcgacgtcgc cgtggatcag tgctccggta gtcaccatct cggtgtaaac
    15781 caccgcatgc ggggacagtt ggcggaaaaa gaaccggcaa tgccggtccg accaatccat
    15841 catcggcgcg gtgcagagac ggtgggaatg cggtgttgat tgtggcatgg ggcgatgcaa
    15901 ttaccgggtt ccggcgatcg tgcgcgaact atttgcaatt gccggtgcag cggataacac
    15961 gctccccgca ggtgatcgtg gcgagccgtt tgccgagccc ggaatcgctg tcgccgccac
    16021 tgtgctcgat gcgcgcgtcg ttgcactgta tttcgacagt cacgaacagc ggctcggtga
    16081 tccattccac gttcaggcac cccggggaac cggtgccgaa tcgcaggccc atgcggtaag
    16141 tgccgcacag caagccggtg tgcttgatcc agccgctcac ctgaaagtcg ccgccgtcgt
    16201 tgatccaggc gtgcgaatcg cccgcgcgaa aatcgaaatc cagccgctct ggtttgggcc
    16261 tggcgtccag cgaatacgtg atctgcgcgg gcgcggaaac gccaacctca tgcgggccga
    16321 gtccttgctc gaccggcacg gcattcccgg ccacaaaagc gccgagtgac agtagtgccg
    16381 cgaggcgagt catgagagtt ttcctggggc ggtatcgggt catcatagcc cgaatcggtc
    16441 gacaaaaaag gcgcaccgca gtacgccttt tcttggtgcc gag
//
LOCUS       DASQOP010000016         3759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2221867, whole genome shotgun sequence.
ACCESSION   DASQOP010000016 DASQOP010000000
VERSION     DASQOP010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2221867"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VE030_00620"
     CDS             complement(<1..300)
                     /locus_tag="VE030_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF21948.1"
                     /translation="MIAPAARMMLFGHARPNVRKLRFLMTDYSDLLREEETKLPPNTR
                     ALFLTHATVFAPGAYRISSLFEQLHRSFRTPTVLFYPGTWNHTLNYMGLRSDDQAL"
     gene            938..2431
                     /gene="istA"
                     /locus_tag="VE030_00625"
     CDS             938..2431
                     /gene="istA"
                     /locus_tag="VE030_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HZF21949.1"
                     /translation="MISPETRVQIRRYFYAEHWKVGTIALQLGLHADTVRQAIEVESF
                     HRTEQLRSSILDPYLEFLRQTLDQYPRLRATRLHQMIRDRGYTGSIRQLRRAVAGLRP
                     RRTEAFLRLQTFPADQAQADWAHFGHVMVGRARRALSCFVITLSWSRALYLEFFFDQM
                     MENFLRGHVHAFESFSGVPRVILYDNLRSAVLERRHDQILFNPRLLELAGHYHFVPRP
                     CQVRAGNQKGRVERAIRYVRDSFWAGRTFTTLEECNRQALQWRDDVAHQRPWPSDHSR
                     PVVQAFGEEQPRLLPLPLHPFPTDKVVDVRSGKTIYVRFDLNDYSIPPAAVGRVFTLA
                     ASATEVRILDGLVEIARHRRSYDRQQFVLNPAHQEALLQLKRKALHSSTPGGRLVQAA
                     PECEKLLDLAFAQGESAGSQTAQLLRLLDLYGAPALRLAVAEVLARDTPRASSVAFVL
                     RKQKRPALPPAVDLSRHPRAQAIDVRPHDLETYDELARTAKNNDPDK"
     gene            2388..3176
                     /gene="istB"
                     /locus_tag="VE030_00630"
     CDS             2388..3176
                     /gene="istB"
                     /locus_tag="VE030_00630"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HZF21950.1"
                     /translation="MNSPAPQRTTIPTSSLPDQLRQIGLRALPAQLDDFLARATKARW
                     SPHQFLEQLTRVENDERSRRSLERRLRLSGLKSFKPMADFDWAWPASIDRDVVERALT
                     LDFLPEARNIVLIGRNGLGKTMIAQNICHAAVQAGHSVLFRSAAALIEDLHRQTTEGR
                     RRKLRTCANVGLLCIDEVGYLSFDDKAADLLYEVINRRYERKAVILTTNRPFKEWNEV
                     FPNATCIATMLDRLLHHADVTVVEGNSYRVRESERETAARRAKP"
     gene            3173..3535
                     /locus_tag="VE030_00635"
     CDS             3173..3535
                     /locus_tag="VE030_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF21951.1"
                     /translation="MTDPAAYIAAVLQLYAELPDTPIHAGVSDQWLARRFFREQIPLN
                     IVEAALLLGSLRRLVRPPGMPPLSPIRSLAYFRPVIDELQTHPLPDAYLDYLRLKLHR
                     ASQTFPAKCQKTTFSDGR"
BASE COUNT          734 a         1206 c         1031 g          788 t
ORIGIN      
        1 aagtgcctga tcgtctgatc gcaatcccat gtagttcagc gtgtggttcc aggtgccggg
       61 ataaaacaag acggtggggg ttcggaagct gcgatgcagt tgttcgaata gcgatgaaat
      121 acgataagcg cccggcgcga ataccgtcgc gtgcgtaagg aaaagagcgc gcgtgttcgg
      181 gggcaacttg gtttcttctt cgcgcagcag gtcgctgtag tcggtcatca gaaaacgtag
      241 ttttctgaca tttggccggg catggccaaa caacatcatc cgcgccgcag gcgcgatcat
      301 ccgtcaatca tcatcggtga aagtggaagt tgagcgtgaa ggtaatgaac cccgccgtta
      361 gtagcggcgg ggttctggct tggtggcgac cttcatggga aagtcaacca cacatcgaag
      421 ctggttctaa cctcccatga acgtgtaacc gttgtcaaag cccgcacgcc gggcgcatga
      481 ctattcatgg tgaggcggtt ctgcgggcgc ggctttgtct gggccgcgcc tgcaacgtgt
      541 tgttctttat ttgttcgaac tgcgaccgcg ggcaacgcta ttgcggtgtg acttgccgcg
      601 taacggcgcg gcggcggcag cgccgttcgg ccaaccgccg acaccagacg agcacggaag
      661 gacgactgga tcatcgcgac cgccaacgcc agtatcggcg acgccggatg caggcccgcg
      721 tgacggatca gggttcccct tcggccatct ctccggcaac atcgaatggt ggagaagaga
      781 ttgcacttgc agcaacaaca accgtagccg taaacgcagc cgcgccagtc attggcatcc
      841 gcactgcaag cggcttttgg ctgcactgtt gctattgcgg tcgtgccggg cgcttcgtca
      901 atccctttcc acgtatccca aaacgaaagc ggcatgaatg atcagtcccg aaacccgcgt
      961 acagatacgc cgttactttt atgccgaaca ctggaaggtc ggcaccatcg ctctgcaact
     1021 cgggctgcat gctgacaccg tgcggcaagc cattgaggtc gagagctttc atcgcaccga
     1081 acagttgcgc tcttccattc tcgatccgta tctggagttc ctgcgccaga ctctggacca
     1141 gtatccccgt cttcgcgcca cgcggcttca ccagatgatc cgggatcgcg gctacaccgg
     1201 cagtatcagg caactgcgcc gcgccgtcgc cggtctgcgc cctcgacgaa cagaagcatt
     1261 cctccgtctg caaaccttcc ccgccgacca ggcgcaggcc gattgggcgc acttcggtca
     1321 cgtcatggtc ggccgtgcca gacgtgcgct gtcgtgcttc gtcatcactc tgtcctggtc
     1381 gcgggctctt taccttgagt tcttcttcga tcagatgatg gaaaacttcc tgcgcggtca
     1441 cgtgcatgcc tttgaatctt tctccggcgt gccgcgcgtc atcctgtatg acaacctgcg
     1501 cagcgccgta ctcgaacgcc gccacgatca gatcctcttc aatccccgcc tgctggaact
     1561 ggcgggacac tatcacttcg ttccacgccc ttgccaggtg cgcgcgggca atcagaaggg
     1621 ccgggtggaa cgtgccatcc gctatgtgcg cgattctttc tgggcgggtc gcactttcac
     1681 cacgctcgaa gagtgcaatc gacaggcgct ccagtggcgc gatgacgtcg cgcaccagcg
     1741 cccatggcct tccgatcact cccgtccggt agtgcaggcc ttcggcgaag agcaaccacg
     1801 tctgttgcct cttcctctcc atcctttccc caccgacaaa gttgtcgatg ttcgctccgg
     1861 caagaccatc tatgtccgtt tcgatctcaa cgactattcc attccgcccg ccgcagtggg
     1921 acgcgtgttc actctcgccg cttctgccac cgaagtgcgg atcctcgatg gtttggtgga
     1981 gattgcgcgc caccggcgca gttacgaccg gcagcagttc gtgttgaacc cggctcatca
     2041 ggaagcgtta ctgcaactca aacgcaaggc gcttcactcc tcaacgcccg gtggccgtct
     2101 cgttcaggcc gcaccggaat gtgaaaagct ccttgatctg gcttttgcgc agggcgaatc
     2161 cgccggcagt cagaccgctc agttgctccg gttactcgac ctctatggag cacccgcact
     2221 gcgccttgct gtggcggaag tactggctcg cgataccccg cgcgcttcct ccgtcgcctt
     2281 tgtgctccgc aaacagaaac gccccgcgct gccgcctgcc gtcgatctca gccggcatcc
     2341 gcgcgctcag gccattgatg tccgccccca tgatctggag acttacgatg aactcgcccg
     2401 caccgcaaag aacaacgatc ccgacaagta gcctgcccga tcaactgcga cagatcggtt
     2461 tgcgagcatt gcctgcacaa ctggatgact tcctcgcgcg cgctaccaaa gcgcgctggt
     2521 cgccgcacca gttcctcgaa caactcacgc gtgttgaaaa cgatgaacgc tcgcgccgca
     2581 gtctggagcg gcgtctgcgg ctgtcggggc tgaagagctt caagcccatg gccgatttcg
     2641 actgggcgtg gcccgccagt atcgaccgcg atgtggtgga acgcgcactg acgctggact
     2701 tcctgccgga ggcgcgcaac atcgtgctga tcggacgcaa cgggctcggt aaaacgatga
     2761 ttgcacagaa catctgtcat gccgccgtgc aggccggcca ctcggtgctg ttccgctcgg
     2821 cggcggcgtt gatcgaagat cttcaccggc aaaccaccga aggccgccgc cgcaagttgc
     2881 gcacctgcgc caatgtcggc cttctttgca tcgatgaggt cggctatctc tcattcgatg
     2941 acaaggctgc cgatctgctc tacgaggtca tcaaccggcg ctacgaacgc aaggccgtca
     3001 ttctcacgac caaccgccct ttcaaggaat ggaatgaagt cttccccaat gccacctgca
     3061 ttgcgaccat gctggaccgg ttgctgcacc acgccgatgt cactgtggtt gaaggcaata
     3121 gttacagggt tcgtgaaagc gaacgggaaa cagcggcgcg gagagcgaag ccgtgacgga
     3181 cccggctgct tacatcgcgg cggttctcca actgtacgcc gagcttcccg atacgcccat
     3241 ccacgccgga gtttcggatc aatggctggc ccgccgcttc tttcgcgagc agattcccct
     3301 caacatcgtt gaggctgccc ttctcctcgg ttcgctccgg cgcttggtgc ggcctcccgg
     3361 catgccgcca ctttcaccga ttcgttcgct cgcctacttc cgcccagtta tcgacgaact
     3421 ccagactcac ccgttgcccg atgcctacct cgattacctc cgcctcaaac tccaccgagc
     3481 ctcacaaacc tttccggcca agtgtcagaa aactacgttt tctgatggcc gttaacacac
     3541 aagtagcccc tggacagttc gaagcagcac tcagcccatc ggagatgtac caaaccgatg
     3601 ggaacacgaa gacttcagca ttttggactg cttatagagt cgcggaagtt cagtgaatta
     3661 acacaggaca aaccacgtgc cacaattcca tcaaacactt tacagcgcgt tgctgcatct
     3721 ttttctgccg acgactcgga ttctactggt cgtttcatc
//
LOCUS       DASQOP010000017         5125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1013744, whole genome shotgun sequence.
ACCESSION   DASQOP010000017 DASQOP010000000
VERSION     DASQOP010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5125
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013744"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1186
                     /gene="glgC"
                     /locus_tag="VE030_00640"
     CDS             <1..1186
                     /gene="glgC"
                     /locus_tag="VE030_00640"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HZF21952.1"
                     /translation="LTDWRTKPAVPFGGKFRIIDFPLSNCVNSGIRRIGIATQYKSHS
                     LIRHIQRGWSFLDGRFEEFVDILPASQRTHEENWYRGTADAVFQNLDILRSYDPDHIL
                     ILAGDHIYKMDYGKLLAYHVDKNADMTVACLEVPVEEARAFGVMAVNEDWRVTAFQEK
                     PAKPPSIPGNPALSLASMGIYVFNAPFLYEQLARDADTPSSSHDFGKDIIPHLVPRYR
                     VFAQRFDDSCVGMDGKPAYWKDVGTVDAYWEANMDLTRVTPDLNMYDTEWPIWTHQEQ
                     LPPAKFVFDDDGRRGMAVDSVVSGGCIISGAKISRSLLFSNVHVHSYSSIEDSVILPD
                     VEVHQHVRLKRVVVEKRCTLPEGLAVGFDPAEDRKRFHVSERGITLITPEMLGQEVLH
                     QR"
     gene            1200..2897
                     /locus_tag="VE030_00645"
     CDS             1200..2897
                     /locus_tag="VE030_00645"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HZF21953.1"
                     /translation="MSGNTKLDLVLLWHMHQPDFRDHATGEYAQPWVYLHAIKDYSDM
                     AAHLENHPKVRAVVNFTPVLLDQLEDYADQFASGRFRDPLLRLLAHPDLYRLDAAERD
                     LVLRCCFKANHHKMIEPYPAYRRLWDFYTLTQQPGTAGETYLSAQYLGDLLTWYHLAW
                     VGETVRRRDQTVPQLLAKAQNFSHADRMQLVELIGRETGAVVPRYHKLAAQGRIELTT
                     SPAFHPIGPLLISFEAAREALPGAPLPQSSHYPGGRTRASAHIAAARESHTRRFGQAP
                     RGMWPAEGAVSQVFVDLLAEGGCEWIASGEGVLVNSLRKNNAPIPERHNFLYRPYRLG
                     GGHAPVTCFFRDDRLSDLIGFEYSKWHGKDAAAHFVGELKGIVEQGDASERRVVSVIL
                     DGENAWEYYPYNGYFFLDELYGALEAHPAIETTTFTRLLDEGRHAEPMPALVAGSWVY
                     GNLATWIGSPEKNRAWDLLCAAKRNFDLVAVSGRLNAQELADATRQLADCEASDWFWW
                     FGDYNPSESVLAFDRLYRKKLAHFYRLLKLPVPAELDVPVSQGGGQAELGGAMRRAS"
     gene            3075..5057
                     /locus_tag="VE030_00650"
     CDS             3075..5057
                     /locus_tag="VE030_00650"
                     /inference="COORDINATES: protein motif:HMM:NF020660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HZF21954.1"
                     /translation="MTKTVSLLFGVHAHQPAGNFEQVLNDAHARCYKPFIETVYRYPD
                     FKLSMHFSGWLLGYLIKHFPEDMAKLREMVTRGQVEMFGGGDTEPVLASIPERDRRSQ
                     LLALSDRLEAAFRQRPSGAWLTERVWESAVGPSLADSGIRYVTVDDYHFLCAGKRSEE
                     LTGYYSTEEGSVRLDLFPISEALRYRIPFSTAPEAIQYLESRATDDGAAAAIYFDDIE
                     KLGIWPETYQWVYEKEWLKLFIEGVLASPLIEPVLYRDFAKQQRTRGVVYLPSTSYIE
                     MNEWTLDADRADAFAALVKQEKDQHRYDSSKPFLRGGIWRNFISRYAESNWMHKRMLQ
                     VSGRVAALGRKAKPRMRELLHLAQSNDAYWHGLFGGIYLPHLRRSVWNAIVELEALLD
                     KASPRKETVLDLDLDGRDEIFLNSGKMQAVVRDDGVGAIHELDFYPLRQNFGDTLARR
                     TEHYYRKIREGGHGGHSGDGIASAHDRVNFKHPVSPEDLIPDPYPRAMFVDRIKDQPQ
                     PIRYALRKASANAIELAGAGLTKKISVKGNTLTVEYRLERESAGFEIEINLAMPSADG
                     FLGRYVVNGEIPGGFAQAFNWEGIKSLTLEDAVLGGKVTLTTPEPVGLGAAPHKTVSQ
                     SEDGFEKIMQAVCLRIHVPAGNSSVKLQITIEPLSG"
BASE COUNT          990 a         1649 c         1571 g          915 t
ORIGIN      
        1 tctcacggac tggcggacga aaccggcggt gcccttcggc ggcaagttcc gcatcatcga
       61 cttcccgctg tcgaactgcg tcaactccgg catccgccgc atcggcatcg ccacccagta
      121 caagtcgcac agcctgatcc gtcacatcca gcgcggctgg agttttctcg acgggcgctt
      181 cgaggaattc gtcgacatcc tgcccgcctc ccagcgcacc cacgaggaga attggtaccg
      241 gggcaccgcg gacgccgtgt tccagaacct cgacatcctg cgctcctacg acccggatca
      301 catcctgatc cttgccggcg atcacatcta caagatggac tacggcaagc tgctcgcgta
      361 tcacgtggac aagaatgccg acatgacggt agcctgcctg gaggtaccgg tcgaggaagc
      421 cagggccttc ggcgtgatgg cggtgaacga agactggcgc gtgactgcgt ttcaggaaaa
      481 acctgccaag ccgccctcga ttccaggcaa tccggcgctg tcgctcgcca gtatgggcat
      541 ctacgtgttc aatgcaccat tcctgtacga acagctcgcg cgcgacgccg acacgcccag
      601 ttccagccac gatttcggca aggacatcat cccccacctg gtgccgcgct atcgcgtatt
      661 tgcacagcgc ttcgacgaca gctgtgtcgg aatggacggc aagccggcgt actggaaaga
      721 cgtgggcacg gtagatgctt actgggaagc gaacatggat ttaacccgcg tcacgccgga
      781 tctcaacatg tacgacaccg aatggccgat ctggacgcac caggagcaac tgccgccggc
      841 caagttcgtc ttcgacgacg acggacggcg cggcatggcg gtggattcgg tggtgtccgg
      901 cggctgcatc atcagcggcg cgaaaatctc gcgctcgctg ctgttttcca acgtgcacgt
      961 gcacagctac agcagcatcg aggactcggt gatccttccc gacgtggaag tgcaccagca
     1021 cgtgcgcctc aagcgcgtag tggtggaaaa gcgctgcacc ctgccagaag gtctcgcggt
     1081 cgggttcgat cctgccgagg accgcaagcg cttccacgtc tccgagcggg gcatcacgct
     1141 gatcacgccg gagatgctgg gccaggaagt gttgcaccag cgctgacgaa tcggacgcga
     1201 tgagcggcaa tacaaagctg gacctggtcc tgctgtggca catgcaccag ccggatttcc
     1261 gcgaccacgc caccggcgag tatgcccagc cctgggtgta tctgcacgcg atcaaggact
     1321 acagcgacat ggcggcgcac ctggagaatc accccaaggt gcgcgcggtg gtcaatttca
     1381 ccccggtgct gctcgaccag ctcgaggact acgccgacca gttcgccagc gggcggtttc
     1441 gcgatccgct cctgcgactg ctcgcgcatc ccgatctgta ccggctcgac gccgccgagc
     1501 gcgacctggt gctgcgctgc tgcttcaagg ccaatcatca caagatgatc gagccctatc
     1561 cggcttatcg gcggttatgg gacttctata ccctgacgca gcagcccgga accgccggcg
     1621 aaacctatct ttccgcgcag tacctcggcg atctgctcac ctggtatcac ctcgcgtggg
     1681 tgggcgagac cgtgcgccgg cgcgaccaga ccgttccgca gttgctggcg aaggcgcaga
     1741 acttctcgca cgccgaccgc atgcagctcg tcgagctgat aggccgcgaa accggcgcag
     1801 tggtgccgcg ctatcacaag ctcgccgcgc agggccggat cgaactcacc accagccccg
     1861 catttcaccc gatcgggccg ctgctgatct ccttcgaggc agcgcgtgaa gccctgcccg
     1921 gtgcgccgct tccgcaaagc tcgcattacc cgggcggacg cacgcgcgcg tccgcgcaca
     1981 tcgccgcggc cagggaaagc cacacgcgcc gcttcggcca ggcgccgcgg ggcatgtggc
     2041 cggccgaagg tgcggtatcg caggtgttcg tggacctgct cgcagaaggt ggctgcgaat
     2101 ggatcgccag cggcgaaggc gtgctggtga acagcctgcg caagaacaac gcgccgattc
     2161 ccgaacggca caattttctg taccggcctt accgcctcgg cggcggccat gcgccggtca
     2221 cctgtttctt tcgcgacgac cgcctttcgg acctgatcgg cttcgagtac agcaagtggc
     2281 acggcaagga cgcggccgcg cacttcgtgg gcgagttgaa aggaatcgtc gagcagggcg
     2341 atgcatccga gcgccgggtc gtcagcgtga ttctggatgg cgagaacgct tgggagtact
     2401 acccctacaa cggctatttc tttctcgacg agttgtatgg cgcactggaa gcgcaccccg
     2461 caatcgagac cacgactttt acgcggctcc tcgacgaggg gcggcacgcg gaaccgatgc
     2521 ccgcgctggt cgcgggcagc tgggtgtacg gcaatctcgc cacctggatc ggctccccgg
     2581 aaaaaaaccg cgcgtgggac ctgctctgtg ccgccaagcg gaacttcgat ctggtggcgg
     2641 tgagcgggcg cctgaatgcg caggaactcg cggacgcgac ccgccagctc gccgactgcg
     2701 aggcatcgga ctggttctgg tggttcggcg actacaaccc cagcgagtcg gtgctggcct
     2761 tcgatcgcct gtaccgcaag aagctcgccc atttctaccg gctcctcaaa ctgccggttc
     2821 ccgccgaact cgacgtgccg gtgagccagg gcggcggcca ggccgaactc ggcggcgcca
     2881 tgcgccgcgc ctcgtaatca tcaccacaga gacacagagg cacggagaag aacaatagaa
     2941 agggttaaca aatgcaacaa agtccgcttc aacaatcaat ttctttcgca cattgcgttg
     3001 tctgagcgcc tttaacgcga gtgattggtt tttctccgtg cctctgtgtc tctgtggtta
     3061 gtccgctcta aaagatgacc aagaccgttt cactcctttt cggagtgcac gcgcaccagc
     3121 ccgcgggcaa cttcgagcag gtcctgaacg acgcccatgc acgttgctac aagcctttca
     3181 tcgaaaccgt ttaccgctat ccggacttca agctgtcgat gcacttcagc ggttggctgc
     3241 tgggttacct gatcaagcat ttcccggaag acatggcgaa gctgcgcgag atggtgacgc
     3301 gcggccaggt ggaaatgttc ggcggcggcg acaccgaacc ggtgctcgct tcgattcccg
     3361 aacgcgaccg ccgctctcag cttctcgcat tgtccgatcg cctcgaagcc gcgttccggc
     3421 aacgcccgag cggcgcctgg ctcaccgaac gggtatggga atcggcggtc ggtccttcgt
     3481 tggccgattc gggcattcgc tacgtcaccg tggacgacta ccatttcctc tgcgccggca
     3541 agcgcagcga ggagctgacc ggctactaca gcaccgagga aggcagcgtc cgcctcgatc
     3601 tgttcccgat ctccgaggcg ctgcgctacc ggataccttt ttccaccgcc cccgaggcga
     3661 tccagtatct cgaatcgcgc gctaccgacg acggcgcggc cgcagcgatc tacttcgacg
     3721 atatcgagaa gctcggcatc tggcccgaga cttaccaatg ggtgtacgaa aaggagtggt
     3781 tgaagctatt catcgagggc gtgctggcat cgccgctgat cgagccggtg ctctaccgcg
     3841 acttcgcaaa acagcaacgc acgcgcggcg tggtgtacct gccctccact tcgtacatag
     3901 agatgaacga gtggacgctg gacgccgacc gggcggacgc attcgccgcg ctggtaaagc
     3961 aggagaagga ccagcatcgt tacgacagca gcaagccctt cctgcgcggc ggcatctggc
     4021 gcaatttcat ttcgcgctac gccgagtcca actggatgca caagcgcatg ctgcaggttt
     4081 ctggccgggt tgccgctctg ggcagaaagg cgaagccgcg catgcgcgag ctgctgcacc
     4141 tggcgcaatc gaacgacgcc tactggcacg gcctgttcgg cgggatctat ctgcctcatc
     4201 tgcgccgttc ggtgtggaat gccatcgtcg agctcgaggc gctgctcgac aaggcatccc
     4261 cgcgcaagga aacggtactc gacctggatc tcgacggtcg tgacgagatc ttcctgaaca
     4321 gcggcaagat gcaggcggtg gtccgcgatg acggagtcgg ggcgatccac gagctggatt
     4381 tctacccgct gcgccagaac ttcggcgaca cgctggcgcg ccgtacggaa cattactacc
     4441 gcaagatccg cgagggcggc cacggcggtc attccgggga cggcattgcg tcggctcacg
     4501 atcgtgtgaa cttcaagcat ccggtttctc cggaagacct gattccggac ccgtacccgc
     4561 gcgcgatgtt cgtcgaccgg atcaaggatc agccgcaacc gattcgctac gcactgcgca
     4621 aggcctccgc gaatgctatc gaactggccg gagccggtct gaccaagaag atttcggtga
     4681 aaggtaacac gctaacggtc gagtaccggc tggagcgcga atcggcgggc ttcgagatcg
     4741 agatcaacct cgccatgccg agcgccgacg gcttcctcgg ccgctacgtg gtgaacggcg
     4801 aaattccggg aggcttcgcg caggcgttca attgggaagg catcaagtca ttaacgctgg
     4861 aagacgcggt gcttggcggc aaagtcacgc tcacaacgcc cgagccggtc ggtctcggcg
     4921 cggcgccgca caagaccgtg tcgcagtcgg aagacggctt cgagaaaatc atgcaggcag
     4981 tctgcctccg cattcacgtg cccgccggta attcttcggt caagttgcag atcacgatag
     5041 aaccgctgtc gggttgacac actgtaatcg cgggtcgaac atggccctgt gacggggaga
     5101 ttcagggcgc gcagctttcc acccg
//
LOCUS       DASQOP010000018         4771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1215359, whole genome shotgun sequence.
ACCESSION   DASQOP010000018 DASQOP010000000
VERSION     DASQOP010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215359"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VE030_00655"
     CDS             complement(<1..453)
                     /locus_tag="VE030_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF21955.1"
                     /translation="MAVTLGTGEFRYEAVEDWAKLPDGWTLRDAAAIAVDSKDQVYVF
                     NRSEHPMMVFDRDGNFLRSWGEGVFRNAHGIHIGPDQTLYCTDDGDHTVRKCTLEGKV
                     LLEIGIPGKPSPYMSGEPFHRCTHTALSPRGDIYIADGYGNARVHKYSP"
     gene            914..1969
                     /locus_tag="VE030_00660"
     CDS             914..1969
                     /locus_tag="VE030_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HZF21956.1"
                     /translation="MPTTTRWKKRPEGSTWGDFGPDDQLGRLNLLTPKKVKQGVAEVK
                     EGLTFCLSLPLDYPGGNVLNPRRHPPILRPTLRNGRPNMVYIVQRDNPDATDVINDDA
                     VIMHLQYSTQWDSLAHVGQLFDADGDGKPEPVFYNGYRAGKEISGPSDPDDAGAIGSV
                     PAKTTTAVHALGIENMAVNCVQGRGVMIDLHAHVGRARAAIGYDQLMRILEKDKVLVE
                     EGDMVCLHTGFAQMILEMNKQPDGPALENACAALDGRDQKLLQWITDSGLVSLIADNY
                     AVEAHPATSHVGSCATLPLHEHCLFKLGVNLGEIWHLTPLANWLREHKRSRFLLTAPP
                     LRLPGAVGSPATPVATV"
     gene            1985..2746
                     /locus_tag="VE030_00665"
     CDS             1985..2746
                     /locus_tag="VE030_00665"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZF21957.1"
                     /translation="MPTHPSAINSVQKACRILRSLTEPRVSRLSEVAGQAVLNKVTTL
                     RILEVLTREGFVRRDEATKTYSLGNEVNILAAALRDRDDLRSRVHPSLLRLAALSEDS
                     VLLSVRSGTEAVCVDREVGTFPIRANYLDIGSRRPLGVGAGAMALLAWQSQAEIEAVL
                     AFVAQRLPHFPKVSLTWLRNEIERTRDRGYALFLNMLVDRMGAVGVPVFGSDGKPIAA
                     LSIAALSDRISSRLDALVEMLKREADIVADVKVKA"
     gene            2874..4010
                     /locus_tag="VE030_00670"
     CDS             2874..4010
                     /locus_tag="VE030_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HZF21958.1"
                     /translation="MSWITGVGLTAYGKHPGRGTLDLMSDAAAAALADAQLERAEVDG
                     LVCGYSTTMPHLMLATVFAEHFGLKPTYAHAIQVGGATGFAMAMLAHHLTESGVARNI
                     LVVAGENRLTGQSRDSAIQTLAQVGHPAYEVPLGATIPAYYGLVASRYIHEYGHREAD
                     LAELAVLMRRHASLHSGAQYREPITVETVLGSKSIASPLKLLDCCPVSDGGAAFVVSR
                     DRSNDFAVRIIGTGQAHTHQHVSAAASLTQFGAASSAARAQEAAGVAIGDIDYAAIYD
                     SFTITLSILLEEIGLAARGAAGRLAAEGHFNHDGKLPLNTHGGLLSYGHCGVGGAMAH
                     LVETHLQLTGRAGKRQARNVRRALLHGDGGVLSSHVSLVVERAA"
     gene            4007..4375
                     /locus_tag="VE030_00675"
     CDS             4007..4375
                     /locus_tag="VE030_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HZF21959.1"
                     /translation="MTAEILDWTRGGAGIAYQTCASCGAVWYFQRGFCPVCGDGNPVT
                     RQASGAGTVYARTSVARAPTEELRAHAPYLIVLVDADEGFRLMAHGDPTLEIGDWVRA
                     RFIDLAGRKIPYFEPADGRR"
     gene            4604..>4771
                     /locus_tag="VE030_00680"
     CDS             4604..>4771
                     /locus_tag="VE030_00680"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HZF21960.1"
                     /translation="MSDSPRDSLRTVLDPKSVAIVGASENPNKLGGRPLLYLSRFGYK
                     GSVYPINSKRAN"
BASE COUNT          874 a         1510 c         1523 g          864 t
ORIGIN      
        1 gggcgagtac ttgtgaacgc gcgcgttgcc gtatccgtcg gcgatataga tatcgccgcg
       61 cggcgacaac gcggtgtgcg tgcagcgatg gaaaggctcg ccgctcatgt atggcgaagg
      121 cttgccgggg atgccgatct ctagcaggac cttgccctcg agcgtgcact tgcgcacggt
      181 gtggtcgccg tcgtcggtgc aatagagtgt ctggtccggg ccgatgtgga taccgtgcgc
      241 gttcctgaac acgccctcgc cccacgaacg aagaaaattc ccgtcgcggt cgaacaccat
      301 catcgggtgt tcgctgcggt tgaagacgta gacctggtcc ttggaatcga cggcgatggc
      361 tgcggcgtca cgcagcgtcc agccgtccgg cagctttgcc cagtcctcca ctgcttcgta
      421 gcggaactcg ccggttccga gcgtgacggc catgttcctc cccccttttc ttgatcggag
      481 cctggattgt cgcggcaggg tcacgactgc gcaactgcgc tcgaggcgct gcctggaata
      541 ttccgaatcc cggaaccaca cccggccatc ctgcttatct cattgtattg acatgccaat
      601 agcaccggac ggaattccgc cggccagaac gactccttga caaacaggac cggcgacgcc
      661 gaagattccg ctgacaaccc gtcgcagagc gggactcaag ggaggcgaat tcatgatgaa
      721 tccgaccgcc acgcgttcac gtggctgttc gcgttgccgg ttgcgccatg cccggccgac
      781 gccggtccgc gtctctccct gtcgtatcaa agaacagtaa gagctgttcg agacaccagg
      841 aaaatccgct gcggacttcg gtccggcgtt gactggtgtt ctcgattcaa acaaaggttt
      901 gaagggagga cagatgccaa cgaccacgcg ctggaagaaa cgaccggaag gctcgacttg
      961 gggagacttc ggtcccgacg atcaactcgg gcggctcaat ctgctgacgc cgaaaaaagt
     1021 gaaacagggt gtggcagagg taaaggaggg gttaaccttt tgcctgagcc tgccgctcga
     1081 ctatccgggc ggcaacgtgc tcaacccgcg ccgccatccg ccgatcttgc gcccgacgct
     1141 gcgcaacggc cgcccgaaca tggtctacat cgttcagcgc gacaaccccg atgccaccga
     1201 cgtcatcaac gatgatgcgg tcatcatgca cctgcaatac tcgacccagt gggacagcct
     1261 ggcgcacgtc gggcagctgt tcgatgccga cggcgacggc aagccggaac ccgtgttcta
     1321 caacggctac cgcgccggca aggaaatctc cggtccttcc gacccggacg atgccggcgc
     1381 gatcggcagt gtgccggcga aaacgaccac ggcggtacac gcgctcggca tcgaaaacat
     1441 ggcggtgaac tgcgtccagg gtcgcggcgt gatgatcgac ctgcatgcac acgtcggccg
     1501 cgcgcgcgca gcaatcggct acgaccagtt gatgcgcatc ctcgaaaaag acaaagtcct
     1561 cgtcgaagaa ggcgatatgg tgtgcctgca taccggtttt gcccagatga tcctggagat
     1621 gaacaagcag cccgacgggc cggcgctgga gaacgcctgt gcggcgctcg acggtcgtga
     1681 tcagaagctg ctccaatgga tcacggattc cgggctggtc tcgctgatcg cggacaatta
     1741 cgcagttgaa gcgcatccgg ccaccagtca tgtcggtagc tgcgcgacat tgccgctgca
     1801 cgaacattgc cttttcaagc tcggcgtcaa tctcggcgag atctggcatc tgaccccgct
     1861 cgccaactgg ctgcgcgaac acaagcgttc gcgctttctg cttactgcgc cgccgctgcg
     1921 cctgcccggc gcggtcggct cacccgcgac acccgtcgct accgtttaga aaaaaggaac
     1981 cgccatgcca acccacccct cagccatcaa ttccgtgcag aaggcctgca ggatcctgcg
     2041 atccctgacc gagccgcgcg tcagccggct gtccgaggtc gccggccagg ccgtgctcaa
     2101 caaggtcacg acgctgcgca tcctcgaagt gctgacgcgc gaaggtttcg tgcgccgcga
     2161 cgaggcgacc aagacttatt ccctcggcaa cgaagtcaac attctcgccg cggccctgcg
     2221 cgaccgcgat gacttacgca gccgcgtcca tccttcgctg ctgcggcttg cggcgctttc
     2281 cgaagactcg gtactgctgt cggtgcgcag cggcaccgaa gcggtgtgcg tggatcgcga
     2341 agtcggcacc tttccgatcc gcgccaacta tctggatatc ggcagccgca ggccgctggg
     2401 tgtgggcgcc ggcgcgatgg cgctgctggc gtggcagtcc caggcggaga tcgaggcggt
     2461 cctggcgttc gtggcgcagc gattgccgca tttcccgaag gtcagcctga cgtggctgcg
     2521 caacgagatc gagcgcaccc gcgaccgtgg ttacgcgctg ttcctgaaca tgctggtgga
     2581 ccgcatgggt gcggtcgggg tgccggtgtt cggttccgac ggcaaaccca ttgcggcgct
     2641 gagcatcgcg gcgctgtccg accgcatttc ctcgcggctg gacgcgctgg tggagatgct
     2701 caagcgcgag gcggacatcg ttgcggatgt gaaggtgaag gcatgaatgg aggggcgagg
     2761 gacgagaggc gaggggcgag gggaggagcg ccgagaggcg gcgcgttttt cctatccccc
     2821 ttcccccctt caccccttca cccattcacc aaattgcacc gaaggagcaa tcgttgagct
     2881 ggatcaccgg agtcgggcta acggcttacg gcaagcatcc cggccgcggc acgctcgatc
     2941 tgatgagcga tgcggccgcg gcggcgctgg ccgatgcgca gctcgaacgc gccgaagtgg
     3001 acggcctggt atgcggctac tcgaccacga tgccgcacct gatgctcgcc acggtgtttg
     3061 ccgagcactt cggactgaag cccacctatg cccacgccat tcaggtcggc ggcgccaccg
     3121 gtttcgccat ggcaatgctg gcacatcacc tgactgaatc cggcgtggcc aggaacatcc
     3181 tcgtggtggc aggcgaaaac cggcttaccg gccagagccg cgactcggcg atccagaccc
     3241 tggcgcaagt tggccacccg gcgtatgagg tgccgctcgg tgcgaccatt ccggcgtact
     3301 acggcctggt cgcatcgcgc tacatccacg agtacggcca ccgcgaagcc gacctcgcgg
     3361 aacttgcagt gttgatgcgc cggcacgcca gtctgcattc cggcgcacag taccgcgagc
     3421 cgatcacggt cgagacggtt ttgggttcca agtcgatcgc ctcaccgctg aaattgctcg
     3481 attgctgtcc ggtctccgac ggcggcgcgg ccttcgtggt gagccgcgac cgcagcaatg
     3541 atttcgcggt gcgcatcatc ggcaccggcc aggcgcatac ccatcagcat gtgagcgccg
     3601 cggccagcct gacgcagttc ggcgccgcat cttcggctgc cagagcgcag gaagcggccg
     3661 gcgtcgcgat cggggacatc gattacgccg cgatttacga cagtttcacc atcacgctct
     3721 ccatcctgct cgaggagatc ggcctggccg cgcgcggcgc ggcgggcagg cttgcggccg
     3781 agggacattt caaccacgac ggaaaactgc cgctcaatac gcacggcggg ttgctctcct
     3841 acggtcactg cggagtgggg ggtgcgatgg cacacctggt ggaaacccat ttgcagctca
     3901 ccggccgggc cgggaagcgt caggcgagga atgtccgccg ggcgctgttg cacggcgacg
     3961 gcggcgtgct gtcttcgcat gtttctctgg tcgtcgagag agcagcgtga cggcagagat
     4021 cttagactgg acccgcggcg gcgcaggcat cgcctatcag acctgcgctt cctgcggggc
     4081 agtctggtat ttccagcgcg ggttctgtcc cgtctgcgga gatggcaatc cggtgacccg
     4141 gcaggcttcg ggggcgggaa cggtgtatgc gcgcaccagc gtcgcgcgtg cgccgacgga
     4201 ggagcttcgc gcgcacgcgc cttacctgat cgtgctggtg gatgccgacg aaggatttcg
     4261 tcttatggcg cacggcgacc cgacgcttga gatcggcgac tgggtgcggg cgcggttcat
     4321 cgacctcgcc ggccgcaaga ttccgtattt tgagccggcc gatggccggc gataaaagcg
     4381 ttgacgcaga gggcgcagag gaaaaacaaa gacgagggaa agcgaggggg ggttataagc
     4441 gggatgactg ttcgcgaacc tgggcgaccg atttttactg cgagggatcg tcaaccaatg
     4501 acagacctta acccgcgcat tttcctgccc ttgcctttcc tccgtgtcct ccgcgtcctc
     4561 cgcgcctctg cgttgggttt gatcttttcg gaggataaca acaatgagtg actccccgcg
     4621 cgattcactc cgcactgtgc tcgatccgaa atccgtcgcc atcgtcggag cttcggaaaa
     4681 cccgaacaaa ttgggtggac gcccgctgct ttatctgtcc cgtttcggtt acaagggcag
     4741 cgtgtatccg atcaattcga agcgggccaa c
//
LOCUS       DASQOP010000019         4821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1416725, whole genome shotgun sequence.
ACCESSION   DASQOP010000019 DASQOP010000000
VERSION     DASQOP010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4821
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416725"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            543..3242
                     /locus_tag="VE030_00685"
     CDS             543..3242
                     /locus_tag="VE030_00685"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZF21961.1"
                     /translation="MPRLRLRLTPHGRLSVEDQDDGPDIDAAASIRLIDAFARGTGYG
                     LVWLGAAEVGQALPPLFVWWRDFAALYVGSLCLHASGMEAEGPRKLPSVPAPTAAELS
                     SLVLTAPIMAGAEYLTQDVLLALWDEMERAFAASLAAAKVGLQAFLTTLNRACNLVGR
                     VHFNLAENRRDPDQPFAFMATYTTRLSAQAKVQHVPLGQALREYAGAANRDKLLSLLL
                     PVQRAAEHCVWLRPMIDAGDIFHPLRWGPTEASRFLQSVPELESAGVVVRMPVSWRAS
                     RPARPQVTATVGSRAPLAVGLDGLLDFQMGVMLDGEPLTEVEVATLLAGTENLVLLRG
                     QWVEVDRARLQRTMEQFHAAEELAARDGLTFAEAMRMLAGAGLTLDDPAATDTVWARV
                     TAGPWLEQTLKELRAPDGDGVDPGPALRGTLRPYQKAGSRWMDLLAGLGLGACLADDM
                     GLGKTIQVLSLLLVEQARGASPRPSLLVAPASLLANWAAEIEKFAPGLKVAIVHPSVM
                     PADQVKAFTAEQASDYDLAITSYGSLLRIPVFAEADWRFVILDEAQAIKNPNARQTKA
                     AKALKARARIVLTGTPVENHFGDLWSIFDFINPGLLGNAKQFTRYTKRLAERTHNPYG
                     PLRELVRPYILRRMKTDKSVIADLPDKTEIKALCGLSRRQAALYGQAVEDLAKSLEAS
                     AGIQRKGIVLAMLMRLKQVCNHPSQWLNDNVWAEKDSGKWARLREIAEVVAARQEKML
                     VFTQFREMTGPLATFLGQIFGRTGMVLHGETAVENRKTLVRRFQEDEAVSFFVLSLKA
                     GGTGLTLTAASHVVHFDRWWNPAVENQATDRAFRIGQKKNVLVHKFICRGTVEEKIDA
                     LIDSKQGLSDELLTGGDEINLTEMTNDDIMRMVSLDLNAAMKE"
     gene            3248..4462
                     /locus_tag="VE030_00690"
     CDS             3248..4462
                     /locus_tag="VE030_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF21962.1"
                     /translation="MSRSFGGWAPYVPVAARRRKAEREMEKLRKKGAVLSPVKIDGRQ
                     IARTFWGKAWCDNLESYRDYENRLPRGRTYVRNGSVVDLQIAPREVTAIVSGSKIYKV
                     KVSIGNVGPARWKTLCADCSGGIDSLVELLQGRFSKGVMERLCRQDTGLFPRPSEIRF
                     TCSCSDYASMCKHIAAVLYGVGARLDENPELLFRLRAVDETELLSDLGSALPDTRTKR
                     ETAQTLVGDDLAALFGLDMEESKASAPSRSAAATSAAPQGKRAKAVENLAVAAAPAVE
                     VKANAVTPAQEKKAGSEQGHRPTAKTSTKARSAQAAQKNTMAPTRSAAKKTVAAPTKS
                     AAKPATAKSSAGIRKAQAKATVSARRRSDSVPVIAAPVPVGQFSSRRPVRSSAAPKPA
                     NSAKRRRRDGDG"
BASE COUNT          893 a         1479 c         1562 g          887 t
ORIGIN      
        1 ttcccgtgtt gcgtgcgctt tccttgtgta catgctgccg ccactacccc ggtgcagcag
       61 atgggcgcct tgtcgctcaa acttgcccat ccgtatcagc cttccccgaa accactaccg
      121 ggtcggcctg cacatcggca ttttcgagga ttgctcggcg tttacttgcg ttgcggcctg
      181 cacactcgcg cggtcaccaa ttcgtgaccg ctatccgggg gcttcagaca ttttgtcgcc
      241 tccatgcctg ccccggttgc ttccggctgg agcggtcgcc gggcggggtt tgcacccgct
      301 ggaaagcgcc gccttgtcac ggcgcacgtg gcgagctgcc gttcccgatt tccccggcct
      361 gtgatggcca tggtttgata gcggaccctc acgcatccgt ggcaaggatc gcggcgaagc
      421 ggcgggtcgc ggtcatcact ccggcatttc cgcttgccgc aacgcgtctg ccacccgggc
      481 atataaatca gtctggctgt tcgactgcta caccagatcg gaaaccgatt taaaatagcc
      541 gcatgcccag actcagattg cggttaacac ctcacggacg ccttagcgtc gaggaccagg
      601 atgatgggcc ggacatcgac gccgcggcgt cgatccgtct gatcgacgcg tttgctcggg
      661 ggacaggata tgggctggtg tggctcggcg cagccgaggt ggggcaggca ttgccccctt
      721 tgtttgtttg gtggcgtgac ttcgctgccc tgtacgtcgg gtcgctttgt ctgcacgcgt
      781 ccggcatgga agcggaaggg ccccgaaagc tgccgagtgt accggcgcca actgctgcgg
      841 aactctcatc tctggtgttg accgcgccga ttatggcggg ggccgaatat ctgacccagg
      901 atgtcttgct ggcgttgtgg gatgagatgg agcgagcctt cgctgcgtct cttgccgccg
      961 caaaggtcgg attgcaggcc ttcctgacca ccctgaatcg ggcatgcaac ctggtcggtc
     1021 gggtgcattt caatctggcg gaaaaccgcc gcgatcccga ccagcccttc gccttcatgg
     1081 cgacctacac cacccgcctg tccgcccagg cgaaggtaca gcacgtcccc ctcggccaag
     1141 cgctgcgcga atacgccgga gcggcgaacc gggacaagtt gctatccttg cttctgccgg
     1201 tgcaacgcgc cgcggaacat tgcgtctggc tgaggccgat gatcgatgcc ggagatatct
     1261 ttcatccgct gcgctgggga cccacggagg cgtcgcgctt cctccagagt gtccccgagc
     1321 ttgaaagcgc cggggtggtt gtgcgcatgc ccgtgtcttg gcgcgccagc cgtcccgccc
     1381 gaccgcaggt aaccgcaaca gtcggtagcc gggcgccgtt ggctgtcggg ctggacgggc
     1441 tgctcgattt ccagatgggc gtcatgctgg acggcgagcc actcaccgag gtcgaggttg
     1501 cgaccctgct cgccggtacc gagaacctcg tgctgctgcg tggtcaatgg gtcgaggtcg
     1561 atcgggcgcg gcttcaacgc acaatggaac agttccacgc agccgaggaa ctcgcggccc
     1621 gcgacggcct caccttcgcg gaggcgatgc ggatgctggc cggcgccggc cttaccctgg
     1681 acgacccggc cgcgaccgac accgtttggg cgcgcgtaac cgccggcccc tggttggagc
     1741 agacgctgaa ggaattacgc gcgccggacg gggatggagt cgatcccggc ccggcgctgc
     1801 gggggacgct acgtccctac cagaaagcgg gatcccgatg gatggacctg ctggcaggcc
     1861 ttggtctcgg cgcctgcctg gccgacgaca tgggtctcgg gaagacaatc caggtgctat
     1921 ctctgctgct ggtggaacag gcgcgcggcg ccagtccccg gccgagccta ttggtggcgc
     1981 cagcctccct gctggccaac tgggcagcgg agatcgaaaa attcgccccc ggcctgaagg
     2041 ttgcgatcgt gcatccttcg gtgatgccgg cggatcaggt gaaggctttc accgccgaac
     2101 aggcgtcgga ctatgatctg gcaattacaa gctatggctc tctgctgcgt atccccgtgt
     2161 tcgcggaggc agattggcgg tttgtcattc tggatgaggc gcaagcgatt aagaatccga
     2221 acgcccgtca gaccaaagcg gcgaaggcgc tgaaggcaag ggcgcggatc gtgctgaccg
     2281 ggacgccggt cgaaaatcac ttcggcgacc tgtggtcgat cttcgatttc atcaacccgg
     2341 gtctcctggg caacgccaaa caattcaccc gctataccaa aagactggcc gagcggacgc
     2401 ataatcccta cggcccgctg cgtgagctgg tgcggcccta catcctgcgt cggatgaaaa
     2461 ccgacaagtc ggtcatcgcc gaccttcctg acaagaccga gatcaaggcg ctgtgcgggc
     2521 tgtcgcgccg gcaagctgcg ctgtacggcc aggctgtcga agaccttgca aagagccttg
     2581 aggcatcggc gggcattcag cggaagggca tcgtcctggc gatgctgatg cgactcaagc
     2641 aggtctgcaa ccatccctcc caatggctga acgacaatgt ctgggcggag aaagacagcg
     2701 gcaaatgggc acgcctgcgt gaaatcgccg aggtcgtcgc cgcgcggcag gagaagatgc
     2761 tggtgttcac gcagttccgc gagatgaccg ggccgctggc caccttcctg gggcagattt
     2821 tcggccgcac cggcatggtg ctgcacggtg agaccgcggt ggagaaccgc aaaaccctgg
     2881 tgcggcgttt ccaggaggat gaggcggtct cattcttcgt gctgtcactg aaggcaggcg
     2941 gcaccggttt gaccctgacc gcggcatccc atgtcgtaca tttcgatcgc tggtggaatc
     3001 cagcggtcga gaaccaggct accgaccgag cgttccggat cggccaaaag aagaacgtcc
     3061 tggtacacaa gttcatctgc cgcggcacgg tagaggagaa gattgacgcc ttgatcgact
     3121 cgaagcaggg tttgtcggat gagcttttga caggcggcga cgagatcaac ctgaccgaga
     3181 tgaccaatga cgacatcatg cgaatggtca gcttggatct taacgccgcg atgaaggagt
     3241 aacgctgatg tcgcgctcct tcggagggtg ggcgccctat gtgcccgtcg cggcgcgccg
     3301 ccgcaaagcc gaacgcgaga tggagaaact ccgcaagaaa ggcgccgtgc tctcgccggt
     3361 gaagatcgac ggacgccaga ttgccagaac gttctggggc aaggcgtggt gtgacaacct
     3421 ggagagttat cgcgattatg agaaccggtt accgcggggg cgcacctacg tgcgcaacgg
     3481 ctcggtagtc gatctacaga tcgcaccccg cgaggtaacg gcgattgtca gcgggtcgaa
     3541 gatctataag gtcaaggtca gcatcggaaa cgtcggcccg gcccgctgga agacgctttg
     3601 cgctgactgt tcggggggga tcgactccct ggtggaactg ctgcagggga gattttccaa
     3661 aggggtgatg gagcggctct gccgtcagga taccggcctg tttccgcggc catcggaaat
     3721 ccggttcact tgcagttgtt cggactatgc gtcgatgtgc aagcacattg cagcggtcct
     3781 ctatggcgtc ggcgcgcggc tcgatgaaaa tccagagttg ttgtttcgat tgcgtgcggt
     3841 ggacgagact gaactgctat ccgatctagg gagcgctctg ccagacacgc ggacgaagcg
     3901 cgagacggcg caaaccctgg ttggtgacga tctcgcggca ctgttcggtc tggacatgga
     3961 ggagagtaag gcttcggctc cttcacgctc ggccgctgcc acatcggccg ccccccaagg
     4021 aaaacgcgca aaagcggttg agaacctggc tgttgccgct gctcccgcag ttgaggtcaa
     4081 ggccaatgcg gtgacgcccg cccaggaaaa gaaagcaggc tccgagcagg gacacaggcc
     4141 cacggcgaag acttcaacca aggctaggtc ggcccaggcg gctcagaaga acacgatggc
     4201 acccacccgg tcagcagcga aaaagaccgt tgccgcccca accaaatccg cagcgaaacc
     4261 agcaacagcg aagtcctcgg ccggaatccg aaaggcacaa gccaaagcca ccgtgtccgc
     4321 tcgccgccga tctgactctg ttccggtgat cgcggccccc gtgccagtcg gccaattttc
     4381 gtctcggcgg ccagtcagat catccgccgc gcccaaaccg gccaacagcg cgaagcggcg
     4441 acggcgggac ggagacggtt gatcatcaat gtaaccgatc gtcgtggtcc ggatatgtcc
     4501 gcttcccaac cgacccagcg atccaccggc gaggtcgtga aggtcgctca gggtcggttt
     4561 gagccgttgg gctcggggtc gcgatggagg aagtcacgtt cgcatgtgac tcgctgccgg
     4621 aggaaaccgg acgaactttc ggtcccgcca accaggcggg tgcgggaacg gccccggctc
     4681 agctcggatt cttctgttgg gcgagccatt ccatcgaacc gggggcccat gtgggttcgg
     4741 ggcgtccgga tgctgcctga cgctccgcct gcagctcttc gtgccgctgg cgagcgagtt
     4801 cataggcgcc ccagcccttc a
//
LOCUS       DASQOP010000020         5355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1014313, whole genome shotgun sequence.
ACCESSION   DASQOP010000020 DASQOP010000000
VERSION     DASQOP010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014313"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VE030_00695"
     CDS             <1..182
                     /locus_tag="VE030_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HZF21963.1"
                     /translation="AGNEFTAADVQMSFPLEAATVRAGLDSRWPNLMAFLGRVHARQA
                     YKRAIEKGGEYQLLR"
     gene            complement(262..978)
                     /locus_tag="VE030_00700"
     CDS             complement(262..978)
                     /locus_tag="VE030_00700"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HZF21964.1"
                     /translation="MPKFRKPHHRAVAGILSAMNVPFLERARCYFGGRTCFALLLDEF
                     RESRDIDFLCSSRDGFRQMRETVTEDSLGRILRRKLPLAREVRADRDGIRTFIAAGDL
                     RIKFEIVLEARIDLKSASDRFLGVPVLDRECLSAEKFLANADRGLDESAKSRDVIDLA
                     FLAARHGIKALEPGMRLAETAYGSAVRHYLDLVLGKFGADSRYASACARSLGIEDLGT
                     LRKGLAKLNSLAGKRTKPNH"
     gene            complement(971..1375)
                     /locus_tag="VE030_00705"
     CDS             complement(971..1375)
                     /locus_tag="VE030_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF21965.1"
                     /translation="MKRPGRPRTSKLTLAEQLRRAKRAQRDRDQAAGLVQLQLKLPRP
                     VAAKLAATARIPGYAQALDVLLDDMVVRLADYPALADLAWNRADEYLPAREAFGLYER
                     NWRFVETGKLDERERALIERLAQRFGAGVIHA"
     gene            complement(1472..4183)
                     /gene="mdeB"
                     /locus_tag="VE030_00710"
     CDS             complement(1472..4183)
                     /gene="mdeB"
                     /locus_tag="VE030_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012406586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate dehydrogenase"
                     /protein_id="HZF21966.1"
                     /translation="MTDISDISVSGLWRVLTQDLDPVETKEWLDAFDAVVENEGPERA
                     TFVLRKLLDHARIVRVPMPPVLNTPYANTVSLADQPQFPGNAEIEARISALVRWNALA
                     MVVRANRAHPELGGHIATYASSADLFEVGYNHFFRAGQNGDAIYFQPHSAPGVYARAY
                     LEGRLTEENLDRYRRETGGKGLSSYCHPWLMPEFWQFPTGSMGLGSITSIYQARFMRY
                     LEDRGILETKGRKVWCFVGDGEMDEPESIAGLSMAAREGLDNLIFVVNCNLQRLDGPV
                     RGNGSIVQELEGLFAGAGWNVVKVMWGSDWDPLFARDRDNVILKRLHETVDGEFQKYA
                     ATDGRFNREAFFSKYPELQQLVAHMSDEDIDRLKRGGHDPVKIYAAYHAAVKHRGRPT
                     VVLAQTKKGYGMGTAAQGKMAAHQAKKLEDDTLLAFRDRFALPLSDSDVHDLRFYKPA
                     ADSPEMKYLHARRQTLGGYVPARAPKSPVLQVPALDSFARLLEGTGDREQSTTMVFVN
                     MLSQLLRDKALGRYIVPIVADEARTFGMQSLFRQVAIYSPHGQLYEPEDKDELLYYKE
                     TKDGQILEEGITEAGAISSWIAAATSYSAHGVPMLPFYIFYSCFGFQRIGDLVWSAGD
                     ARARGFLLGATAGRTTLSGEGLQHEDGSSHLLFSTVPNCVAYDPAYGYELTVIIRDGM
                     RRMLENQEDVFYYVTVMNENYAHPAMPQGAEDGILKGMYRLREGRGARGERRGRKQTP
                     QVQLLGAGTILREVLAAAELLESDWNVGADVWSVTSFTELRRDGIGVERHNRLHPVGN
                     QKQSWVEQCLEKTSGPVIAASDYMRTVADLIRNWVPRRYVALGTDGYGRSDIRAALRD
                     FFEVDRRHVAVAALKALADDGSIDAKTVQQAIEKYAIGADRPNPWEV"
     gene            4348..4896
                     /locus_tag="VE030_00715"
     CDS             4348..4896
                     /locus_tag="VE030_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011494178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZF21967.1"
                     /translation="MPDMQLDAIDWRILGLLQGDARLSNVELAKAVGLSPSPCLNRVR
                     ALEQAGYISRYVTLLDALRVGLKVSVFIQVTLERQIEPALETFEKAIRGRPEVMECYL
                     MTGDADYLLRVVVPDIQVLEDFILNFLSRVPGVGNIKSSFALKQVKYQTALPLPLPAA
                     AGTRREARGVRSAERRPTKGRR"
     gene            complement(5102..>5355)
                     /locus_tag="VE030_00720"
     CDS             complement(5102..>5355)
                     /locus_tag="VE030_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF21968.1"
                     /translation="LAVAAPAWGADPEYDGQCALGMAEGTPISTDCTVVWLSQEGKIY
                     CFFNQAAKEKFLLAPQENLQRAQAFWDNPAVLKQIRRQQ"
BASE COUNT          949 a         1670 c         1754 g          982 t
ORIGIN      
        1 ttgccggcaa cgagttcacg gccgccgacg tccagatgag ctttcccctg gaggcggcca
       61 ccgtgcgcgc cggtctggat tcccgctggc cgaatctgat ggcattcctc ggtcgcgtcc
      121 acgcgcgcca agcctataag cgggcgatag aaaaaggcgg cgagtatcag ttgctgcgct
      181 gatcccgctg cgagagcagg cagccgagat ggcgctcatt acctacgaga tctgagggag
      241 cggcccgaga tacagggcgt ttcagtgatt cggtttcgtc cgtttgcccg ccaaagaatt
      301 cagcttcgca agtcctttgc gcagggtacc gagatcttcg atgcccagcg agcgtgcaca
      361 ggccgacgcg taccgggaat cagccccgaa cttcccaagc acaagatcga ggtagtgtct
      421 aaccgcggag ccgtaggcgg tctccgcgag ccgcatgccg ggctcgagcg ccttgatgcc
      481 atggcgcgcg gcgaggaagg cgaggtcgat gacgtcgcgg gactttgccg attcgtccag
      541 tccccgatcc gcgttcgcca gaaatttctc cgccgacagg cattcgcgat cgagcaccgg
      601 aacgccgagg aagcgatcgg acgcgctctt cagatcgatc cgcgcctcga gtacgatctc
      661 gaacttgatg cgcaagtcgc cggcggcgat gaaggtccgt atgccgtccc ggtcggcacg
      721 cacttcgcgg gccagtggca gcttgcggcg caggatgcgg cccagggaat cctcggtcac
      781 ggtctcgcgc atttgccgga agccgtcccg gctcgagcac aggaaatcga tgtcccgcga
      841 ttcgcggaac tcgtcgagca gcagcgcgaa gcaagtcctg ccgccgaaat agcagcgtgc
      901 ccgctccagg aacggcacat tcattgccga caggatgcca gcaacggccc gatgatgcgg
      961 cttgcggaac ttaggcatgg atcacgccgg cgccgaagcg ttgcgccagc ctttcgatca
     1021 gagcgcgctc ccgctcgtcc agcttgccag tctcgacgaa gcgccagttg cgctcataca
     1081 agccgaacgc ttcccgcgcc ggcagatatt cgtccgcgcg gttccacgcc aagtctgcca
     1141 gcgccgggta atcggcgagc cgcacgacca tatcgtcgag caacacgtcg agcgcctgcg
     1201 catatccggg aatgcgggca gtcgcggcca acttggcggc gacaggccgg ggaagcttga
     1261 gctgaagttg caccaacccc gccgcttggt cgcggtcgcg ctgcgcccgc ttggcgcggc
     1321 gcagttgttc ggcaagggtg agcttgctgg ttctgggtct gccggggcgt ttcacggggc
     1381 aagcccgccg gtgttatgtt gtcatgtcaa cataatgaac gggctgaccg gccagacagg
     1441 acgcacaaac cacacgccgg cccgaggcgc actagacctc ccaggggttg ggccggtcgg
     1501 cgccgatggc gtatttctcg atcgcctgct ggacggtctt ggcgtcgatc gagccatcgt
     1561 cggcaagcgc cttgagcgcg gcaaccgcga cgtggcggcg atcgacttcg aagaaatcgc
     1621 gcagtgcggc gcggatgtcg cttctgccgt agccgtcggt gccgagcgcg acataccggc
     1681 gcggtaccca gttgcggatc aggtccgcta ccgtgcgcat gtaatcgctg gcggcgatca
     1741 ccgggccgga agtcttttcc aggcactgct cgacccagga ctgtttctgg tttccgaccg
     1801 ggtgcaggcg gttatggcgt tcgacgccga tgccgtcgcg gcgcaattcg gtgaaactgg
     1861 taacgctcca cacatcggcg ccgacgttcc agtcgctttc cagcagctcc gccgctgcga
     1921 gcacctcgcg caggatcgtc cccgcaccca ggagctggac ctgcggggtc tgctttctcc
     1981 cgcgtcgctc gcccctcgcc cctcgcccct cgcgcaagcg gtacattccc ttcagaatcc
     2041 cgtcctccgc gccctgcggc atcgcaggat gcgcgtagtt ctcgttcatc accgtgacgt
     2101 aatagaacac gtcttcctgg ttttcgagca tacggcgcat gccgtcgcga atgatgaccg
     2161 tgagttcgta accgtatgcg gggtcgtagg caacgcagtt cggcacggtg gagaacagca
     2221 ggtggctgga tccgtcctcg tgctgcaggc cttcgccgga aagcgtggtg cgtcccgcgg
     2281 tggcgccgag cagaaatccg cgggcgcgcg cgtcgcccgc ggaccacacc aggtcaccga
     2341 tgcgctggaa gccgaagcag gaatagaaga tgtagaacgg cagcataggg acgccgtgtg
     2401 cgctgtacga ggtcgcggcg gcgatccatg aggaaatcgc tccggcctcg gtgatgcctt
     2461 cctcgaggat ctggccgtcc ttggtctcct tgtaatagag cagctcgtcc ttgtcctccg
     2521 gctcgtagag ctggccgtgc ggagagtaga tcgccacctg gcggaacagc gactgcatgc
     2581 cgaaggtgcg cgcctcgtca gcgactatcg gcacgatgta ccggcccagc gccttgtcgc
     2641 gcaacagttg cgacagcatg ttcacgaaca ccatggtggt ggactgctcg cgatcgccgg
     2701 tgccttccag cagccgggcg aaactgtcca acgcaggcac ctgcaacacc gggcttttcg
     2761 gcgcgcgcgc cggaacgtaa ccaccgagag tttgccggcg ggcgtgcaga tacttcatct
     2821 ccgggctgtc tgcggccggc ttgtagaagc gcaggtcgtg cacatcggaa tcggaaagcg
     2881 gcagcgcgaa ccggtccctg aaggcgagca gcgtatcgtc ctcgagcttc ttcgcctggt
     2941 gcgcggccat cttgccctgc gccgccgtgc ccatgccgta gcccttcttg gtctgggcga
     3001 ggaccacggt ggggcggccc ctgtgtttta ccgcggcgtg atacgccgcg tagatcttga
     3061 ccgggtcgtg gccgccgcgc ttcagccggt cgatatcctc gtcggacatg tgcgccacca
     3121 gttgctggag ttccgggtat ttgctgaaga aggcttcgcg gttgaagcgg ccgtcggttg
     3181 ccgcgtactt ctggaattcg ccgtcgacgg tctcgtgcag ccgcttgagg atgacgttat
     3241 cgcggtcgcg cgcgaacagc gggtcccagt ccgagcccca catcaccttg accacattcc
     3301 agccggcgcc ggcgaacagc ccctcgagtt cctggacgat cgagccgttg ccgcgcaccg
     3361 ggccgtccaa gcgctgcagg ttgcagttca ccacgaaaat gaggttgtcc agaccctcgc
     3421 gcgccgccat cgacaggccg gcgatggatt cgggctcgtc catttcgccg tccccgacga
     3481 aacaccacac cttgcggccc ttggtttcga ggatgccgcg gtcctcgaga tagcgcatga
     3541 agcgcgcctg gtagatcgag gtgatcgaac ccaggcccat cgagccggtg ggaaactgcc
     3601 agaactccgg catcagccac gggtggcagt acgaggacag ccccttgccg ccggtctcac
     3661 gccggtaacg atcgagattt tcctcggtca gccggccctc gagataggcg cgggcgtaaa
     3721 cgcctggcgc ggagtgcggc tggaagtaaa tcgcatcgcc attctggccg gcgcgaaaga
     3781 aatggttgta gccgacttcg aagagatcag cggatgaggc gtaagtggcg atgtggccgc
     3841 cgagctcggg gtgggcgcga ttggcgcgca ccaccattgc cagcgcattc cagcgcacca
     3901 gcgcggaaat ccgtgcttcg atttccgcgt tgccggggaa ctgcggctgg tcggcgagcg
     3961 aaaccgtgtt cgcatatggc gtgttcagca ccggcggcat tggcacgcgc acgatgcgcg
     4021 cgtgatccag cagttttctg agtacgaagg tggcccgctc cgggccctcg ttctcgacca
     4081 ctgcgtcgaa ggcatccagc cattccttgg tctcgaccgg atcgaggtcc tgcgtcagaa
     4141 cgcgccacag gccggaaacg ctgatgtcgg aaatatcggt catgcgattt ctcctcgcgg
     4201 ccgccgcatc aggcgatggc ggcccggtgt tcgattttcg aagccggaat gctaacgcga
     4261 ttcatgaagc acgtggtttc gtttaccctg gtaaaatcga tggagttaag catagatgac
     4321 tgttaatcat caatgaaaca gcacgaaatg ccggatatgc agctcgacgc gatagactgg
     4381 cggatcctcg gactgctgca gggcgacgcc cgcttgtcga acgtggagct cgccaaggcg
     4441 gtggggctgt cgccttcgcc ctgcctgaac cgggtgcgag cgctggagca ggcgggctac
     4501 atcagccgct acgtgacgct gctcgatgca ctgcgggtcg ggctgaaggt gagcgtgttc
     4561 atccaggtca cgctggagcg gcagatcgag ccggcgctcg agaccttcga gaaggcgatc
     4621 cgcggcaggc ccgaggtcat ggagtgctat ctcatgaccg gggacgcgga ctatttgttg
     4681 cgcgtagtgg tgcccgacat ccaggttctc gaagacttca ttctaaactt cctctctcgc
     4741 gtgccggggg tgggcaacat aaagtcgagc tttgctttga agcaggtgaa gtaccagacg
     4801 gcgctgccgt tgccgctgcc ggcggccgca ggcacgaggc gcgaggcacg aggggtaaga
     4861 agcgctgagc gtaggccgac caagggccgg cgttaagcgc gttcaacgca gaggatgcgg
     4921 aagaaaagca cgagagggcg agaggggaaa acgagagtat tggcggcgac ttgattggcg
     4981 cttgtctcgc gcctcgtgcc tgacgcctgg tgcctcgtga aacgttgcgg ccgcgacccg
     5041 gcaggaggcg gcaggtgagg agccgattaa cctgcgcccg taggcggttc gaagcctcgc
     5101 gttattgctg gcgcctgatt tgtttcagaa cggccggatt gtcccagaat gcctgcgcgc
     5161 gctgcagatt ctcctgcggc gccagcagga acttctcttt ggcggcctga ttgaaaaagc
     5221 agtaaatctt tccttcttgc gagagccaga cgaccgtgca atccgtggat atcggggttc
     5281 cttccgccat gcccagcgcg cattgaccgt cgtattcggg gtcggcgccc caggcgggcg
     5341 ctgctaccgc gagca
//
LOCUS       DASQOP010000021         4158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2021860, whole genome shotgun sequence.
ACCESSION   DASQOP010000021 DASQOP010000000
VERSION     DASQOP010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2021860"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..301
                     /gene="groES"
                     /locus_tag="VE030_00725"
     CDS             8..301
                     /gene="groES"
                     /locus_tag="VE030_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HZF21969.1"
                     /translation="MKIRPLHDRVIVKRLEEERKTASGIVIPDTAAEKPDQGEIIAVG
                     HGKVLEDGKVRPLDVKKGDKILFGKYSGQTVKVHGEELLVMREEDIMGVVEAA"
     gene            349..1998
                     /gene="groL"
                     /locus_tag="VE030_00730"
     CDS             349..1998
                     /gene="groL"
                     /locus_tag="VE030_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006753915.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HZF21970.1"
                     /translation="MAAKDVKFHDSARARIVAGVNILADAVKVTLGPKGRNVVLERSF
                     GAPTITKDGVSVAKEIELKDKFENMGAQMVKEVASKTSDVAGDGTTTATVLAQAIVQE
                     GMKFVAAGMNPMDLKRGIDKAVVAVVEELKKISKPCTTGKEIAQVGSISANADSSIGE
                     RIAEAMEKVGKEGVITVEDGSGLADELDIVEGMQFDRGYLSPYFINNPERQISILENP
                     YVLLHDKKISNIRELLPVLEQVAKAGRPLLIIAEDVDGEALATLVVNNLRGILKTCAV
                     KAPGFGDRRKAMLEDIATLTGGTVIAEEVGLTLEKATLKDLGQAKRVEIGKEETTIID
                     GAGDTKAIEGRVKSIRKQIDEATSDYDKEKLQERVAKLAGGVALIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGIVAGGGVALIRARSAIAKLKGDNHDQDAGIKIVMRALEEP
                     LRWIVANAGDEPSVVLNKVVEGKGNFGFNAATGEYGDMVEMGVLDPTKVTRYALQNAA
                     SVAGLILTTAAMVAEAPKEEHSHGGGGGGMGGMGGMGGMDM"
     gene            2261..3883
                     /locus_tag="VE030_00735"
     CDS             2261..3883
                     /locus_tag="VE030_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZF21971.1"
                     /translation="MTGVASATSIHDKNHPDFKPEEKRVIFASSLGTVFEWYDFYLYA
                     VLAPFFAALFFPPGNQTAALLSAFATYAAGFLVRPFGALVFGRVGDLVGRKYTFLITI
                     VVMGASTFAVGLLPTFETIGWFAPVLLVTLRLLQGLALGGEYGGAATYVAEHAPNHRR
                     GYDTSWIQTTATLGLFLALVVIYLCRHNMDAAEFKNWGWRIPFWGSVILLAFSVYIRL
                     KLEESPVFKKMKDEGKGSKAPLTDSFLRYPNNKYVLLALLGATAGQGVVWYTGQFYAL
                     FFMIITLRLDYVTTYTLIGLSLLIGTPFFIVFGWLSDKIGRLKIIMAGCLIAALTYFP
                     LFQGLTHYVNPALEQYQQNVKITVAASDCSFHIFVGPWSKFSDCDRTKDFLTKQGLSF
                     TSAPGIEGKSVVATIGDTKIEGWDEKKLNDALKATGYPGPADTTKVNYGMTLLILVIM
                     VIYVTMVYGPIAAFLVELFPTRIRYTSMSLPYHIGNGWFGGMLPLTATAMVAATGDIY
                     YGLWYPIVVAVMTLIVGILFLKETKDRDIHQN"
BASE COUNT          880 a         1204 c         1282 g          792 t
ORIGIN      
        1 agttgagatg aaaatccgtc cattgcacga ccgagtaatc gtcaaacgct tggaagagga
       61 gcgcaagacg gcttccggca tcgtgatccc cgacaccgct gcagagaagc ccgatcaggg
      121 cgagatcatt gcggtgggcc acggcaaagt tctcgaagac ggcaaggtcc ggccgctgga
      181 tgtgaagaaa ggcgacaaga tcctgttcgg caagtactcc ggccagaccg tcaaggttca
      241 cggcgaagaa ctgctggtaa tgcgcgaaga agacatcatg ggcgtggtcg aagccgcctg
      301 agtcgattcg aaaagttcca ctacatctaa attcaggagc aaattcctat ggcagctaaa
      361 gatgtcaaat ttcatgactc ggcccgcgca cgcattgtgg ccggcgtcaa catactggcc
      421 gatgcggtca aggtgacttt gggcccgaag ggccgcaacg tggtgctgga gcgctccttt
      481 ggcgccccga ccatcaccaa ggacggtgtt tcggttgcga aagaaatcga actcaaggac
      541 aagttcgaga acatgggcgc acagatggtg aaggaagtcg cctccaagac ttccgatgtg
      601 gccggcgacg gcaccaccac cgccaccgtg ctggcgcagg cgatcgtgca ggaaggcatg
      661 aaattcgttg ccgccggcat gaacccgatg gatctcaaac gcggtatcga caaggcggta
      721 gtcgccgtgg tcgaggagct caagaagatc tccaagccct gcaccaccgg caaggaaatc
      781 gcccaggtcg gttcgatttc cgccaatgcc gattcttcaa tcggtgagcg catcgccgag
      841 gcgatggaaa aggtcggcaa agaaggcgtg atcaccgtcg aggacggctc tgggcttgcg
      901 gacgagctgg atatcgtgga aggcatgcag ttcgaccgcg gctacctgtc gccgtacttc
      961 atcaacaatc cagaaaggca gatctctatt ctggaaaacc cctacgttct tctccacgac
     1021 aagaagatct ccaacatccg cgaactgctg ccggtgctgg aacaagtcgc caaggccggc
     1081 cggccgctcc tgatcattgc cgaagacgtc gacggcgaag cgctcgccac cttggtggtc
     1141 aacaacctcc gcggcatcct caagacctgc gcggtgaaag cgccgggctt cggcgatcgc
     1201 cgcaaggcca tgctggaaga catcgcgacc ctgaccggcg gtacggtgat tgccgaggaa
     1261 gtcggcctta ccttggaaaa ggcgaccctc aaggacctcg gccaggccaa gcgcgtcgag
     1321 atcggcaagg aagaaaccac cattatcgat ggcgccggcg acaccaaggc gatcgaaggc
     1381 cgcgtcaagt cgatccgcaa acagatcgac gaggcaacca gcgattacga caaggaaaag
     1441 ctccaggagc gcgtagcgaa actcgcgggc ggcgtggcgt tgatcaaggt cggtgccgct
     1501 actgaagtcg agatgaagga gaagaaggcc cgagtcgagg atgcactgca tgcaacccgt
     1561 gctgcagtcg aggaaggcat tgtcgccggt ggtggcgttg cgctgatccg tgcccgctcc
     1621 gcaattgcca agctcaaggg cgacaaccat gatcaggatg ccggcatcaa gatcgtgatg
     1681 cgcgcactgg aagagccgct gcgctggatc gtcgccaacg ccggcgacga gccttccgtg
     1741 gttctcaaca aggtcgtcga aggcaagggc aacttcggct tcaacgccgc gaccggtgag
     1801 tacggggaca tggtggagat gggcgtgctc gaccctacca aggtcacccg ctacgcgctg
     1861 cagaatgcgg cctccgtggc tggcctgatc ctgaccacgg ctgcgatggt tgccgaggcg
     1921 ccgaaggaag aacattccca cggcggcgga ggcggtggaa tgggcggcat gggtggcatg
     1981 ggtggcatgg acatgtaatt ccgggtcctg gcccgttcga agaagccccg cgcgagcggg
     2041 gctttttttt gtgcggccgc aacaccggtc aactctgccc gcaatggctg cgttctctgc
     2101 gggcgctgcg cccctgggga agcgcatctc gttgacaatt gcggaggcgg ataatatgtt
     2161 gagtcgccgc gccgccggtt ggcgcgcagc ggaatcccgg gccttcgtga cgacggtctg
     2221 gcagcggtaa ccaaaagaac agtaacccag ggagaagaac atgactggag tcgcatccgc
     2281 caccagcatt cacgacaaga atcacccgga tttcaaaccg gaggagaaac gggtcatctt
     2341 cgcttcttcc ctgggcactg ttttcgagtg gtacgacttt tatttgtacg cggtgttggc
     2401 gccattcttc gccgcactgt tctttcctcc gggaaaccag accgctgcgc tgctttcggc
     2461 gttcgcgacc tacgccgcgg gttttctggt ccgaccgttc ggcgcattgg tatttggccg
     2521 cgtcggcgac ctggtcggac gcaagtacac cttcctgatc accatcgtcg tcatgggcgc
     2581 ctcgactttc gcggtcgggc tgctaccgac gttcgaaacc atcggctggt tcgcgccggt
     2641 ccttctggtg acgctgcgct tgctgcaggg tctggcgctg gggggtgaat acggcggtgc
     2701 ggcgacttat gtggcggaac acgcccccaa ccaccggcgt ggttacgaca ccagctggat
     2761 ccagaccacc gccacgctgg gcttgtttct ggcgctggtg gtcatttacc tgtgccgcca
     2821 caacatggac gctgcggagt tcaagaactg gggctggaga attccatttt gggggtcggt
     2881 gatcctgctg gccttctccg tgtatatccg gctaaaactc gaagaatcgc cggtgttcaa
     2941 gaagatgaag gacgagggca agggctccaa ggcgccgctg accgacagtt tcctgcgcta
     3001 tccgaacaat aagtacgttc tgctcgcgct gctgggcgcg accgcgggcc agggcgtagt
     3061 gtggtacacg ggccagttct acgcgctgtt cttcatgatc atcacgctca ggctcgacta
     3121 tgtcaccaca tacacgctga tcggcctttc gctgctgatc ggaacgcctt tcttcattgt
     3181 cttcggctgg ctttccgaca agatcggccg gctcaagatc atcatggccg gttgcctgat
     3241 cgcggcgctg acttatttcc cgttgtttca ggggctgacc cattacgtca atcccgcgct
     3301 cgagcagtac cagcagaatg tcaagatcac ggtggcggcg tccgactgca gtttccacat
     3361 tttcgtcgga ccgtggagca agttcagcga ttgcgaccgg accaaggact tcctgaccaa
     3421 gcaggggtta tcgtttactt cggctcccgg aatcgagggc aagtcggtgg tagccacgat
     3481 cggcgacacc aagatcgaag gctgggacga aaagaaactc aacgacgcgc tgaaggccac
     3541 cggctatccc gggccggcag acacaaccaa ggtcaactat ggcatgacgc tgctgatcct
     3601 ggtcatcatg gtgatctacg tcacgatggt gtacgggccg atcgcggcgt tcctggtgga
     3661 attgtttccg acgcggatcc gctatacgtc gatgtctctg ccctaccaca tcggcaacgg
     3721 ctggttcggg ggcatgctgc cgttgacggc cacggcgatg gtcgcggcca ccggggatat
     3781 ttactacggc ctgtggtatc cgatcgtcgt cgccgtgatg acattgattg tcggaatcct
     3841 gttcctgaag gaaaccaaag acagggacat ccatcagaac tgacgcgcaa tccgccccgc
     3901 ttcgaaaagc ccgcacgcgc gggcttttcc gttcatgggc gcacgatccg gatcgacccc
     3961 gcgcgcgcgt tttcgggtaa aatgccgcgc tttccgagcg gcgcttcctg gcgcaacgac
     4021 gcgtcgccaa gtagctcagt cggtagagca caggactaac aatatgccaa tcaaggattg
     4081 gcatattgca gagaaggcta acctgaacac tggtggtcag gttaagcgga gcggccggaa
     4141 ctacaacacg ccgcctta
//
LOCUS       DASQOP010000022         7353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_209571, whole genome shotgun sequence.
ACCESSION   DASQOP010000022 DASQOP010000000
VERSION     DASQOP010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7353
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209571"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..1139)
                     /gene="denD"
                     /locus_tag="VE030_00740"
     CDS             complement(174..1139)
                     /gene="denD"
                     /locus_tag="VE030_00740"
                     /EC_number="1.1.1.410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-erythronate dehydrogenase"
                     /protein_id="HZF21972.1"
                     /translation="MKVFITGGGGFLGYRLALRLLERNTLAGPDGKPATVSRIRLFDA
                     AFPPNPDPRLECVKGDIAEPAAVAAAMDADTGSVFHLAAVVSAGAEADFDLGYRVNLD
                     GTRNLLEACRKLKSPPRLVYASSLAVFGGKLPAVVDDSITPNPQTSYGTQKVIGEYLV
                     NDYSRKGFLDGRSLRLPTIVVRPGKPNLASTSFASGMFREPLNGVVCEVPVDDSTELW
                     ILSPRQVIESFVHAHDLPSSAWGTNRSINLPGITISVKQGVEALRRIAGDEVAARVVF
                     KPVERIQKMIVTFPARFKTSRALEMGFKADTDIRSIIEAYIKEQG"
     gene            1438..2508
                     /locus_tag="VE030_00745"
     CDS             1438..2508
                     /locus_tag="VE030_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZF21973.1"
                     /translation="MIFRQLFDPQSSTYTYLLGDSRSREAVLIDTVFEQARRDAALVS
                     ELELRLLYTLESHIHADHVTGAWILKRRFGSRIALAQSSGAEGADRYLAHGDEIEFGG
                     RHLRVRATPGHTNGCLTYVLDDESMAFTGDCLLIRGSGRTDFQLGDPHALYRSVHTQI
                     LTLPQNCLLYPAHDYRGLTVTSVAEERRFNPRLGGEIGETDFAGYMRNLGLPHPKQID
                     VAVPANLKCGQREHEDVSLTAPTWAALTYTFAGIWEIQPQSLEEAADKVQIVDVREPS
                     EYYGPLGHIRGALMIPLGELARRTGELDRERPVVAVCRSGARSAQATVILQQAGLGVA
                     ANLAGGMLRWRADGHAVEGGSN"
     gene            2669..3565
                     /locus_tag="VE030_00750"
     CDS             2669..3565
                     /locus_tag="VE030_00750"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HZF21974.1"
                     /translation="MTAYPVTESFAAEIGDIDLSKPLSSEERSEIEAAFNRYSVLVFP
                     DQHLSTDQHLEFARNFGPLETTIHATRKDAKLRVRPEIADVSNLDAGESIWGEESRKR
                     MFEMGNRMWHTDSSFRRLPAKASLLYARSIPPVGGQTEFADMRAAYDALPDETKQRLR
                     GLVAEHAIMHSRKKLGFTDFSDEENQTLPPVPQVMVRRHAGSGRMGLYLASHAGRIFG
                     MKEDDGQALLLQLIEHATKRQFVYTHRWRLHDLVMWDNRCTMHRGKGYDDLRWPRDMQ
                     RATVSDVAPTCEQEGIADPVAA"
     gene            complement(3913..4188)
                     /locus_tag="VE030_00755"
     CDS             complement(3913..4188)
                     /locus_tag="VE030_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZF21975.1"
                     /translation="MKNTILQHLRNNGEQLDVDIAKALHIPKTLVLNHISELSSAGEV
                     ICCQVTRYVGGKQIEGVSCRLSAYVPPRATGPKPGAKRGAVTDLSAA"
     gene            4535..6055
                     /locus_tag="VE030_00760"
     CDS             4535..6055
                     /locus_tag="VE030_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006498389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZF21976.1"
                     /translation="MGEEQKLSGPDLSEGVALSELGDGKMMLGHAGGEAVLLARRGEE
                     IFAVGATCTHYSGPLSEGLLVDGTVRCPWHHACFDLRTGAPLRAPALNPIPCYEVMRQ
                     RGRVRVGKRLPEPKLPASPDGAPGQIVILGAGAAGHCAAETLRREGYRGRLVMVGADA
                     DLPVDRPNLSKDYLAGSAPEEWLPLRPREFFDEHGIELVLGVRARSVETARKQLIIES
                     GGTYGYDKLLLATGADPVKLDLPGGDLPHVHYLRTLGHSRAIIAAAGSARHAVVIGSS
                     FIGLEVAASLRARNLEVHVVGPDAVPLARVMGPELGAFIRKVHEEHGVTFHLGTRPRA
                     IDAKTVTLDNGTRVPAELVVIGVGVRPELSLAEKAGLATDRGVLVDEYLQTSAADVFA
                     AGDIARWPDRHSGQRIRVEHWVVAGRQGQTAARNMLGKREKFDAVPFFWSAHYDVGIN
                     YVGHASAWDRIEIEGSFDERNAAVRFIAGEKTVALATIYRDQESLQFERQIELRAT"
     gene            complement(6061..6759)
                     /locus_tag="VE030_00765"
     CDS             complement(6061..6759)
                     /locus_tag="VE030_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006758094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZF21977.1"
                     /translation="MALQGKIVVVTGGFGQLGQAVVRAALDAGAQVAALDRARPPADA
                     TLAAGAFALGELDLAEADAAVRALDQVTSRFGGLDALVNVAGTFRWETLEKGSLDAWD
                     LLYRVNLRSAVSTSRAALPHLFRRGGGRIVNIGAGAAAAKAGAGMGAYTASKAGVAKF
                     TEALADELKDRNVTVNAVLPSIIDTPANRADMPNADFSRWVTPQAIADVIVFLLSEQA
                     RAVTGALIPVSGRV"
     gene            complement(6903..>7353)
                     /locus_tag="VE030_00770"
     CDS             complement(6903..>7353)
                     /locus_tag="VE030_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZF21978.1"
                     /translation="WLLGDEAQSVYATGSCLVQKEIGEAGDVDSTAVTIRTRTGRLCQ
                     INTTRRAAYGYDQRFEVLGAKGQLQARNHKPTEVVAHTALSVSEDKPEHFFLERYRAA
                     YALEIEHFFDALTHGKPVRTTLQDGVKALELADAATRSWQEGRIVTL"
BASE COUNT         1356 a         2313 c         2325 g         1359 t
ORIGIN      
        1 gcgaatcttc tggtccatgt tgtctgttcc cggttgtctg gtcttcgagc gacgtcgcca
       61 atttacgatc gcgctgaggc cagcaatgaa gtcgtggtcc gacctatccc tcacccttca
      121 cccttcaccc ttcacccttc acccttcacc cttcaccctt cacccttcat ccttcaccct
      181 tgctctttaa tgtaagcctc gatgatgctg cgaatatccg tatcggcctt gaagcccatt
      241 tccagcgccc gcgatgtctt gaagcgcgcc ggaaacgtca cgatcatttt ctgaattcgc
      301 tcgacgggct tgaacaccac gcgcgccgca acctcgtcgc cggcgatgcg acgcagcgct
      361 tcgacgccct gcttcaccga gatcgtgatg cccggaaggt tgatcgaacg attcgtaccc
      421 cacgcggacg acggcagatc gtgcgcatgc acgaacgact cgatgacctg cctgggcgac
      481 aggatccaga gctcggtcga atcgtccact ggaacctcgc agacgacgcc attcaatggc
      541 tccctgaaca tgccgctcgc gaacgaagtc gaggcgagat tgggcttgcc ggggcgcacg
      601 acgatggtcg gcagccgcag gctgcggccg tcgagaaagc ctttgcgcga gtaatcgttg
      661 accaggtact cgccgatcac cttctgcgta ccgtaggagg tctgcgggtt gggcgtgatc
      721 gaatcgtcca ccaccgccgg caatttccct ccgaacacgg cgagcgaact ggcgtacacc
      781 agcctcggcg gagatttgag tttccggcag gcctcgagca gattgcgcgt gccgtcgagg
      841 ttgacgcggt agccgagatc gaaatcggct tcggcgcccg cgctgaccac cgctgccaga
      901 tgaaaaaccg aacccgtatc ggcatccatc gcggccgcga ctgcggcagg ctccgcgatg
      961 tcccccttca cgcactccag cctgggatcg ggattcggcg ggaaagcggc gtcgaacagc
     1021 ctgatcctgg aaacggttgc gggcttgccg tcaggcccgg ccagggtgtt gcgctccagc
     1081 agccgcagcg ccagccgata gccaaggaat ccgccgccgc cggtgatgaa caccttcatg
     1141 tcgttttctc caggcaaagg aaaatcttac gcctgtgccg gagagcttgc ggaagcttcg
     1201 actctgaatg cccggctacc gcgggaaagg atcaccgcga ttacagttca gtctctcgct
     1261 ggcgtcttcg atcctgccca aatgtggaca acagcgccat ccgcctgtct ggtactcacg
     1321 aagggtaaac tatattcggg atcagggatt cgtacccgcg ggatgccagg ccatagcttt
     1381 cctgatcatc gattgacgcg aacgcagttg caggttcaga cgccgggacg tggaatgctg
     1441 atattcaggc agttattcga cccgcagtcc tccacctata cctacctcct cggcgacagc
     1501 cgcagtcgcg aggcggtact gatcgacacg gtcttcgagc aggcgcgccg ggacgcggca
     1561 ctggtgagcg aactcgagct gcgcctgctt tacacgctgg aatcccatat acacgctgac
     1621 catgtcacgg gcgcatggat tctgaagcgg cgattcggca gccggatcgc gctggcgcaa
     1681 agcagcggcg ccgagggtgc cgaccgctat cttgcacatg gcgacgagat cgaattcggc
     1741 ggacgccatc tccgggttcg cgccacgccg ggtcacacga atgggtgcct gacctatgtg
     1801 ctcgacgatg aaagcatggc atttaccggg gactgtttgc tgatccgggg cagtggacgc
     1861 acggatttcc agctaggtga cccgcacgca ctctaccgtt cggtccacac ccagattctc
     1921 acgctgccgc aaaattgtct gctctacccg gcgcatgact atcgcgggct caccgtgacg
     1981 agcgtggcgg aggagcggcg attcaatccg cgcctgggag gcgagatcgg cgaaaccgat
     2041 ttcgccggct acatgcggaa cctgggcttg ccgcatccga agcagatcga tgttgcggtg
     2101 ccggccaacc tcaagtgcgg acagcgggag cacgaggatg tgtcgctcac ggcgccgacc
     2161 tgggcggcgc tgacttacac cttcgcgggc atctgggaaa tccagccgca atcgctggaa
     2221 gaagccgccg acaaggtgca gatcgtcgac gtgcgggagc cgtccgaata ctacgggccg
     2281 ctgggacata tccgcggcgc gttaatgatc ccgctcggtg aactggccag acggaccgga
     2341 gagctcgacc gcgagcgccc ggtcgtggcc gtgtgccggt cgggcgcccg ctcggcgcag
     2401 gcaaccgtga tcctgcaaca agccgggctc ggagttgcag cgaacctcgc gggcggcatg
     2461 ttgcgctggc gtgcggacgg tcatgccgtg gagggcggca gcaactaacg atggatttgt
     2521 ggagatcggc ccggtgcgac acggcatagg gattgccgaa cccgggagaa ttcaaattgc
     2581 ttcagcgaac aaaagcagga ggataattga cataaatgcc ggtgccgcta tagtttccgg
     2641 acacctgcaa ccgaagtgga agaaaccaat gaccgcttat cccgtaaccg aaagcttcgc
     2701 cgccgagatc ggcgatatcg acctctcgaa accgctgtcg agcgaagaac gcagcgaaat
     2761 cgaagcggcg ttcaatcgtt actcggtgct ggtctttccc gaccagcatc tgtcgaccga
     2821 ccagcacctc gagttcgcgc gcaacttcgg cccgctggaa accaccatcc atgccacgcg
     2881 caaggacgcc aagctgcgtg tccgcccgga aatcgcggac gtctccaatc tggacgccgg
     2941 cgagtcgatc tggggcgagg aaagccgcaa gcgtatgttc gaaatgggca accggatgtg
     3001 gcatacggac agctcattcc gccgcctgcc ggcgaaagcc tcactcctgt acgcgcgcag
     3061 cattccgccg gttggcggcc agaccgagtt cgccgacatg cgcgcagcct acgacgcgct
     3121 tccggatgaa acgaagcagc gtctgcgcgg gctggtcgcc gagcacgcca tcatgcactc
     3181 gcgcaaaaaa ctgggcttta ccgacttctc cgacgaggaa aaccagacgc tgccgccggt
     3241 gccgcaagtc atggttcggc ggcacgccgg gtcgggacgg atggggttgt atctggcttc
     3301 ccacgccgga cgcatcttcg gtatgaagga ggacgatggc caggcgctcc tgctgcaact
     3361 catcgaacac gcgaccaagc ggcagttcgt ctacacgcac cgctggcgcc tgcacgacct
     3421 ggtgatgtgg gacaaccgct gcaccatgca tcgcggcaag ggttacgacg acttgcgctg
     3481 gccgcgggac atgcagcgcg ccaccgtgtc ggacgttgcg ccgacgtgcg aacaggaagg
     3541 catcgcggat ccggttgcgg cttagtttcg aagcgggtca gtgccctgcg cctgaactcg
     3601 acgcggcggt gctgtaccaa cgacgcatcc accgccaagg acgccaagga aagacaatag
     3661 cgtgtaggcg aggctgagtt aggctaacgg gccagttgcg acgcagatac ttgagcgggc
     3721 ttaaacactc atttcgtttt tgaatttggt tttccctggc gtcccttgcg gttggcgctt
     3781 ggccgtaatc cgaacaacca caaaagaaaa cgggcaccga ctggtgcccg ttgcatttga
     3841 tccacgtacg ccgaaattcg ctagcactcc atgtgatgcg aggcaagacg ttgcgaagat
     3901 cgattcagtg cgtcatgcag cgcttaaatc agtgacggcg cctcgcttgg ctccgggctt
     3961 gggcccggtg gctcgcggcg gcacgtaggc ggacaggcgg caactcacgc cctcgatctg
     4021 ctttccgccg acgtatcgtg tcacttggca gcagatcacc tctcccgcgg atgaaagctc
     4081 cgaaatgtgg ttcagaacca gggtcttagg aatgtggagc gccttggcaa tgtcgacgtc
     4141 cagttgttcg ccgttgtttc tcagatgctg aagaatggtg tttttcatcc aggtatccct
     4201 aggctgcctt cgggtgacgg ggctgcgaat tttaggctac agacgtgatt caggcaagtc
     4261 tcatcctgtg cagacttgtt gggtaagcag aggttcacgt aaaagtcttc ttgacttcag
     4321 actcgttgtg tcatcgggtc ccggacattc ggagcaagat gaggcccagg cagaaccaag
     4381 ccgggtattc gggacacttt ttggaagagg acaatagcag gggaagcgtg ccatgcgact
     4441 ggcgcatcgg agccaaacga ggcaagcgcg cgatagaact gcacttggtc gggcgatgcg
     4501 ctaagttgtc catgcctctt tcacggaaga ccctatgggc gaagagcaga agctgagcgg
     4561 accggacctg tccgaagggg ttgcgctgtc cgagctgggc gacggcaaga tgatgcttgg
     4621 ccacgcgggc ggcgaggcgg tcctgctggc gcgccggggc gaggaaattt tcgccgtcgg
     4681 cgccacctgc acccattatt cgggaccgct cagcgaggga ctgctcgtcg acgggacagt
     4741 acgttgcccg tggcaccatg cgtgcttcga tctgcgcacc ggcgcgccgc tgcgcgcgcc
     4801 ggcgttgaac ccgatcccgt gctatgaggt gatgcgccag cgcgggcggg tgcgggtggg
     4861 caaacgcttg ccggagccga agctgccggc cagccccgat ggcgcgccag ggcagatcgt
     4921 tatcctgggt gcaggggctg ccggacactg cgccgccgaa actctgcgcc gtgagggtta
     4981 ccgcggccgg ctggtgatgg tcggtgcgga cgcggacttg cctgttgatc gtcccaattt
     5041 gtccaaggat taccttgccg gctctgcgcc cgaagaatgg ctgccgctgc gtccacgcga
     5101 gttctttgat gagcacggca tcgagttggt gctcggcgtg cgggcgcgtt ctgtcgagac
     5161 cgcgcgcaaa caactcatca tcgagagcgg cggcacctac ggttacgaca agctgctgct
     5221 cgctaccggc gccgatcctg tcaagctgga tctgcccggt ggcgatttgc cgcacgtgca
     5281 ttatctccgc acactcggcc acagccgcgc catcatcgcc gccgccgggt cggcccggca
     5341 cgctgtcgtc atcggctcca gcttcatcgg cctggaggtc gccgcctcgc ttcgcgcgcg
     5401 caacctcgag gtgcacgtcg tcggccccga tgcggtaccg cttgccaggg tcatgggccc
     5461 ggagctcggc gcttttatcc gcaaggtcca cgaagagcat ggggtgacgt tccatctggg
     5521 aacgcgcccg cgggccattg acgcgaagac ggtgacgctc gacaacggca cgcgcgtgcc
     5581 cgccgagctg gtggttatcg gagtcggcgt gcggcccgag ctgtctttgg ccgagaaggc
     5641 cggccttgcc acagaccgcg gcgtgctcgt cgacgaatac ctgcagacca gcgcggccga
     5701 tgtcttcgcc gccggcgaca tcgcccgctg gcctgatcgc cactcgggcc agcggattcg
     5761 ggtcgagcac tgggtggtcg ccgggcgaca ggggcagaca gcggcgcgca acatgctcgg
     5821 caaacgggag aagttcgacg cggtgccgtt cttctggagc gcgcattacg acgtgggcat
     5881 caactacgtt ggccacgcca gcgcctggga ccgaatcgag atcgagggca gcttcgacga
     5941 acgcaacgcg gcggtccgct tcatcgccgg tgaaaagacg gtcgcgctgg cgaccattta
     6001 ccgcgatcag gagagcctgc agttcgagcg gcagatcgaa ttgcgcgcga cgtaacgcgt
     6061 ctatacgcgt cccgacaccg ggatcaaggc gccggtcacc gcgcgtgcct gttcggaaag
     6121 caggaacacg atgacgtcgg cgatggcctg gggcgtaacc cagcgactga agtccgcatt
     6181 gggcatgtcc gcgcgattcg ccggcgtatc gatgatgctg ggcagcacgg cgttgaccgt
     6241 gacgttgcgg tccttcagtt catcggccag cgcttcggtg aacttggcca ccccggcctt
     6301 cgaggcggta taagcgccca tgccggcgcc agccttggcc gcggcggcac cggctccgat
     6361 gttgacgatg cgtccgccgc cgcgtcgaaa cagatgcggc agtgccgccc gcgaggtgct
     6421 cacggcgctg cgcaaattga cccgataaag cagatcccag gcgtccaggc tgcccttttc
     6481 gagcgtctcc cagcggaagg tgccggcgac gttgaccagc gcgtccaggc cgccgaagcg
     6541 gctggtgacc tggtccaggg cccgcacagc ggcgtcggcc tcggcgagat cgagttcgcc
     6601 cagcgcgaac gcgccggcag ccagcgtggc atcggccggt ggccgggcgc gatccagtgc
     6661 cgccacctgc gcgcccgcgt cgagcgccgc acgcacgacc gcctgaccca gttgcccgaa
     6721 gccgcccgtc accaccacga tcttgccttg caacgccatg atgtcctctt gtcctccgac
     6781 aatattgcca gcgcaaatcc acgaacaatc acgccgctct taatccgata tccttttctt
     6841 cagttgtctt tccttcgcgt cctttgcgtc ctttgcggtg aatgtatttc gagtatttca
     6901 gattagagcg tgacgatgcg gccttcctgc catgaacgcg tcgccgcatc ggcgagttca
     6961 agcgccttga caccgtcctg cagcgtggtt cgcaccggct tgccgtgggt caacgcgtcg
     7021 aagaaatgct cgatctcgag cgcgtaagcg gcgcggtagc gctcgagaaa gaaatgctct
     7081 ggcttgtcct cgctaacgct cagggcggta tgcgcgacga cttccgtcgg cttgtggttg
     7141 cgtgcctgca gttgcccctt cgcgccgagc acttcgaagc gctggtcgta gccgtaggcg
     7201 gcgcggcgcg tggtgttgat ctggcacagg cgccccgtcc tcgtgcggat ggtcaccgcg
     7261 gtggaatcaa cgtcgccggc ctcgccgatt tccttctgca ccaggcaact gccggtggcg
     7321 tacacgctct gcgcttcgtc gccgagtagc cag
//
LOCUS       DASQOP010000023         2378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_9072, whole genome shotgun sequence.
ACCESSION   DASQOP010000023 DASQOP010000000
VERSION     DASQOP010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9072"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="VE030_00775"
     CDS             complement(<1..947)
                     /locus_tag="VE030_00775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010115858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZF21979.1"
                     /translation="MDRAESHQQIRAAVRDLCSRFPGEYWRKLDEARAYPEEFVRALT
                     DAGWLSALIPEEYGGAGLGITEASIILEEVNRSGANSGACHAQMYIMGTLLRHGSDAQ
                     KRRYLPDIAAGRLRLQSFAVTEPTTGSDTTRLKTMAVRNGDRYIVNGQKVWTSRVQHS
                     DLMLLLARTTPLDQVQNKSDGLSVFLVDLRTSGGKGVTVRPIRNMPNHETNEVFLDNL
                     EVPAANLIGEEGKGFRYILDGMNAERILIAAECIGDGHWFIERASRYASERVVFERPI
                     GQNQGVQFPIARAHVNVEAADLMRYKAATLFEAGQPCGA"
     gene            complement(1020..1328)
                     /gene="grxD"
                     /locus_tag="VE030_00780"
     CDS             complement(1020..1328)
                     /gene="grxD"
                     /locus_tag="VE030_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HZF21980.1"
                     /translation="MDAQEKIKQQVTGNPIVLYMKGTPDAPMCGFSAAAVQILEACGV
                     ENVATVDVLADQDIRQGIKDYSKWPTIPQLYVNGEFVGGTDIMREMYQSGELQKLLGK
                     "
     gene            complement(1412..2278)
                     /gene="prmC"
                     /locus_tag="VE030_00785"
     CDS             complement(1412..2278)
                     /gene="prmC"
                     /locus_tag="VE030_00785"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HZF21981.1"
                     /translation="MIATVGALLEKAVRDLVADRDVADARRESQVLLGFALGVSRAWL
                     TTHPDHAADSESAAKFRSLVALRVSGHPVAYLLGVKEFYGLPLRVTPDVLIPRPETEE
                     LVEAALARLPQGEARKVLDLGTGSGCIAIAIARQRPAAKVAAVDSSSPALALARENAA
                     ALDVEAEFLQSNWFAALGRRRFDLIVANPPYVAADDPHLREGDLRFEPRPALAAGADG
                     LADLRRIVGDAPKHLRPGGWLLLEHGYDQAEACRDLLHDAGFGELVFRADIAGLPRIA
                     GGRLLTPDSPSR"
BASE COUNT          414 a          789 c          739 g          436 t
ORIGIN      
        1 tccgccccgc agggctggcc tgcctcgaac agcgtggccg ccttgtagcg catcagatcg
       61 gcggcctcga cgttgacgtg ggcccgcgcg atcggaaact ggacgccctg gttctggccg
      121 atcggacgct cgaacaccac gcgctcgctg gcatagcgcg aggcgcgctc gatgaaccag
      181 tgcccgtcgc cgatgcattc tgcggcgatc aggatgcgct cggcgttcat gccgtcgagg
      241 atgtagcgga aacccttgcc ctcctcgcca atcaggttcg ctgccggcac ttcgagattg
      301 tcgaggaaca cttcgttggt ctcgtggttc ggcatgttgc ggatcggccg caccgtaacg
      361 cctttgccgc ccgaagtgcg cagatccacc aggaacaccg acaggccgtc ggacttgttc
      421 tgcacctggt ccagcggcgt ggtccgcgcc agcagcaaca tcaggtcgga gtgctgcacg
      481 cgggaggtcc agaccttctg cccattgacg atgtagcgat cgccattgcg aaccgccatg
      541 gtcttgagcc tggtggtatc ggagccggtg gtcggctcgg tcaccgcgaa cgactgcagc
      601 cgcagtcttc ccgcggcaat gtccggcaga tacctgcgct tttgcgcgtc ggaaccgtgg
      661 cgcaacagcg tgcccatgat gtacatctgc gcatggcagg cacccgagtt ggcgccggag
      721 cgattcacct cttcgaggat gatcgaggcc tcggtgatgc cgagcccggc cccgccatac
      781 tcctccggaa tcagtgccga cagccatccg gcgtcggtca gcgcacgcac gaactcttcg
      841 ggataggcgc gtgcttcgtc gagtttgcgc cagtactcgc cggggaaacg cgagcacagg
      901 tcgcgcaccg ccgcgcggat ttgctgatgg gattctgccc ggtccattat cgtggggact
      961 gtcctgccac tgaaacaaaa agggctggct cgacggccag cccttttcac tgggtctgct
     1021 tattttccga ggagtttctg cagctcaccg gactgataca tctcgcgcat gatgtccgtg
     1081 ccgccgacga actcgccatt gacgtacagc tgcggaatcg tcggccactt ggagtaatcc
     1141 ttgatgccct ggcggatgtc ctgatcggcc agaacatcga cggtggctac attctcgacg
     1201 ccacaggcct cgagaatctg cactgctgcg gcgctgaaac cgcacatcgg cgcgtccggt
     1261 gtgcccttca tgtacagcac gatcggattg ccggtaacct gctgcttgat cttttcctgt
     1321 gcatccattg gcgatatccc tagaagatta aaagggagtg cccgttccgg atcatgcccg
     1381 gcggagacac tcccgttaga cggcaacgat gttaacgact cggcgaatcg ggcgtcaata
     1441 ggcgcccgcc agcgattcgc ggcaatcccg caatatcggc acggaatacc agctcaccaa
     1501 agcccgcgtc atgcaaaagg tctctgcaag cctcggcctg atcgtagccg tgctcgagca
     1561 gcagccagcc gcccgggcgc agatgcttcg gcgcgtcgcc gacgatacgg cgaaggtccg
     1621 cgagtccgtc cgcacctgcg gcgagcgccg ggcggggctc gaagcgcagg tcgccctctc
     1681 gaaggtgtgg gtcgtctgcg gcaacgtacg gcgggtttgc cacgatcagg tcgaagcggc
     1741 ggcggcccaa tgcggcgaac cagttgcttt gcagaaactc cgcctctacg tcgagtgcgg
     1801 ctgcgttctc gcgcgccaat gccaacgcgg gactcgaact gtccaccgcg gcaacctttg
     1861 ccgcaggccg ttggcgcgcg atcgcgatgg cgatgcatcc gctgccggtg ccgagatcca
     1921 gcaccttgcg cgcttcgccc tgcggcagcc tcgccagcgc cgcttccacc aactcctcgg
     1981 tttcgggccg ggggatgagc acgtcgggcg tgacccgcaa cggcagcccg tagaattcct
     2041 tcacaccgag caagtaggcc accgggtgac cactcacccg gagtgccaca agagagcgga
     2101 attttgcagc ggattcggaa tccgccgcgt ggtccgggtg cgtggtgagc caagcgcgcg
     2161 atacgcccag cgcgaaaccc aacagcacct gcgattcgcg ccgcgcgtcg gccacgtccc
     2221 ggtctgcgac cagatcccgc accgctttct cgagaagcgc gccgaccgtt gcaatcacgt
     2281 ttgggtcgcc accaggtctt ctgccattgc ggcaagcagt tcggcctgat gctcggccat
     2341 cagcgcgccg atcaactcgt cgagtgcgcc gtccatga
//
LOCUS       DASQOP010000024         3891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_816079, whole genome shotgun sequence.
ACCESSION   DASQOP010000024 DASQOP010000000
VERSION     DASQOP010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816079"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VE030_00790"
     CDS             complement(<1..314)
                     /locus_tag="VE030_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZF21982.1"
                     /translation="MIEVAMFGAGRIGKIHGANLGAQPGVKLRYVVDVSAAAAGELAQ
                     RLGAKVADADAAFKDTAVKAVVIASSTDTHAELIMRAAAAGKAIFCEKPVDLALERAR
                     AC"
     gene            complement(936..1943)
                     /locus_tag="VE030_00795"
     CDS             complement(936..1943)
                     /locus_tag="VE030_00795"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HZF21983.1"
                     /translation="MKKTLIVAAMAGLFGLPGVAGAADGKLVFALVPKNMNNPFFDQA
                     RDGCKKAEKEMAGKVECVYIGPGEHGGGEEQVQVVQDLITKKVDGIAVAPSNAPAMAK
                     ALERAKTAGIPVITWDSDLLDKDKGLRATYVGTKNYDIGVNLAKIAMKLKPKGGTICI
                     QSGGPAAANHNERMKGIRDVIAGKVSNTPPPGDRLTGQNGWKEVDGCPLYTNDDFPLS
                     VKQLGEILAKYPDLTAFVPTGGFPQFVPQAYRQVVEKNMDRIKSKKTILVVADTLPMQ
                     MDLLKAGYSHGQVGQRPFDMGYKAMSILKDLKEKKKVPDPIYTGLDVCEPQTAASCLA
                     K"
     gene            complement(1978..2970)
                     /locus_tag="VE030_00800"
     CDS             complement(1978..2970)
                     /locus_tag="VE030_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZF21984.1"
                     /translation="MSVSSEEIAQRRENSPLRTMVRSQPFWVTVALAMICVVMSQISN
                     VFFTEDNFFNVTRNFAFIGIIAMGMTAVIITGGIDLSVGSVVGLSGIVTGLVLNAGHS
                     LWAGIAAGLLTALLCGLVNGLLIAYVRLSSFIVTLGMFGIARSLAQVLSENHMIYEFG
                     PDEKLLFALGGGTIYGFANPFILLAVLTVVFMIVFRYTTWGRWVFALGGNEHAAHLTG
                     VPVNRMKLSVYMLSALMAGISAVLLVGWQGAAINAMGTGYELRVIASSVIGGADLMGG
                     AGGAYGAFIGAALIEVIRNSLLLAGVDANWEGTFVGLFIVFAVLLSKFRGMRSG"
     gene            complement(2985..3725)
                     /locus_tag="VE030_00805"
     CDS             complement(2985..3725)
                     /locus_tag="VE030_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZF21985.1"
                     /translation="MRLFELRGISKKFGAIQALTEVDLKIDAGEVLGLMGDNGAGKST
                     LIKVMAGNFLPSSGEIWLKGERVEFHNPVDARKKGVEVVYQDLALCDNLTAAENVFLG
                     REAHRRIGPFNFIHYAAMFREAARHFAELKSETRPRDFVKQMSGGQRQAVAIARTRLS
                     DPDIVLMDEPTAAISVRQVAEVLDLIRRLRDQNVAVILISHRMPDVFAVCDHVSVMRR
                     GKVVADKPIGQTSPEEVTGLITGAIETA"
     gene            complement(3736..3870)
                     /locus_tag="VE030_00810"
     CDS             complement(3736..3870)
                     /locus_tag="VE030_00810"
                     /inference="COORDINATES: protein motif:HMM:NF016885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0048029 - monosaccharide binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU domain-containing protein"
                     /protein_id="HZF21986.1"
                     /translation="MAKLEGIERFAFYERVKNCFAVVATGERRLYGNLILKKGIIRPD
                     "
BASE COUNT          712 a         1258 c         1122 g          799 t
ORIGIN      
        1 atgcatgcgc gcgcacgctc gagggcgaga tccaccggct tttcgcagaa gatcgctttt
       61 ccggccgcgg ctgctcgcat gatcaactcg gcatgcgtgt cggtgctgga agcgataacc
      121 accgctttca ccgccgtatc cttgaatgcc gcgtccgcat ccgcaacctt ggcaccaagc
      181 cgctgtgcca gctcgcccgc cgcggcagcg cttacatcca ccacgtacct gagcttgaca
      241 ccgggttgcg cccccagatt ggcgccgtgg atcttgccga tgcgcccggc accgaacatc
      301 gccacttcga tcatgcttcc tccctactag tgtcctgctc cgattgctgc attgcgatgc
      361 gatcggaccg tcgtacaggg tgacggtaga ttgtacgaag tttcgatcag ggtggaaaat
      421 aggggaacga agtaaatctt caatttcacc acgaaggcac gaagacacga agaagagcgc
      481 aaatctgcga gagagggcaa aggtccagct tgcaaaacgg ataacggaat tcaatctgtc
      541 atccgtcaaa aatccaggac cgacatctac ccgccgtttc ctcttttctt cttttgaccg
      601 gatcttgcat ttcttcgtgt cttcgtggtg atactttcag ttgccctaga tttcaggccg
      661 tcacaacggc gggcgaacgg gaggcagtgc aggagcagaa cgatccgctt gaccgccgcg
      721 caggcgctgg tgcgttaccc gcggcgctgc gagcggagtg gttgttcgta gatctgtgcc
      781 gccacctttc gcagtgacgc gtcatcggcc ccggaagaat tccaatccga gcactcttgt
      841 tctttttgct ttttccttgg cgtccttggc ggtgaaaatt ccttaaaaaa acggccaccg
      901 ctaaagcggt ggccgcctga tgtcgaagtt cgcgactact tggcgaggca gcttgccgcg
      961 gtttgcggct cgcacacgtc caggccggtg tagatcggat cggggacctt cttcttctcc
     1021 ttgaggtcct tcaggatgct catcgccttg tagcccatgt cgaacgggcg ctggccgacc
     1081 tggccgtggc tgtacccggc cttcagcagg tccatctgca tgggcagcgt atcggccact
     1141 acgaggatgg tcttcttgct cttgatgcgg tccatgttct tttccaccac ctgccggtaa
     1201 gcctgcggca cgaactgcgg gaatccgccg gtcggcacga acgccgtgag gtcaggatac
     1261 ttggccagga tctcgccgag ctgcttcacc gacagcggga aatcgtcgtt ggtgtacagc
     1321 gggcagccat ccacttcctt ccagccgttc tgtccggtca ggcggtctcc gggcggcggc
     1381 gtgttgctga cttttccggc gatgacgtcg cggatgcctt tcatgcgctc gttatggttc
     1441 gccgctgccg ggccaccgga ctggatgcaa atggtgccgc ccttgggctt gagcttcatc
     1501 gcgatcttcg ccaggttcac gccgatgtcg tagttcttgg tgccgacata ggtggcgcgc
     1561 aggcccttgt ccttgtccag caggtcggaa tcccaggtga tcaccggaat gccggccgtc
     1621 ttggcgcgct ccagcgcctt ggccatcgct ggcgcgttgg aaggcgccac cgcgatgccg
     1681 tcgaccttct tggtgatcag gtcctgcacg acttgcacct gctcttcgcc gccgccgtgc
     1741 tcgcccgggc cgatatacac gcactcgacc ttgccggcca tttccttctc ggccttcttg
     1801 cagccgtccc gcgcctggtc gaagaacggg ttgttcatgt ttttcggaac cagcgcgaac
     1861 accaacttgc catccgccgc accggcgaca ccaggcaggc cgaacagccc cgccattgct
     1921 gccacaatca acgtcttttt catcagttcc tcctcatgtc gtaatcggcc cgggttatca
     1981 ccccgagcgc atcccacgga acttgctcaa caataccgca aacacaatga acagacctac
     2041 gaacgtacct tcccagtttg catccacacc ggccagaagc aaactgttcc ggatcacttc
     2101 gatcagggcc gcaccgatga atgcaccgta cgcgcctccc gccccgccca tcagatccgc
     2161 gccgccgatt accgacgacg cgatcacccg caactcgtaa cccgtgccca tggcgttgat
     2221 cgccgcgccc tgccagccga ccagcaagac cgccgagatt cccgccatca gcgcggagag
     2281 catgtagacc gacagcttca tgcggttcac cggcacgccg gtcaggtgcg cggcatgttc
     2341 gttgccgccg agcgcgaaca cccagcgccc ccaggtcgtg tagcggaaca cgatcatgaa
     2401 cacgacggtc aacacggcca gcaggatgaa cgggttggcg aagccgtaga tcgtgccgcc
     2461 gccgagcgcg aacaacagtt tctcgtccgg gccgaactcg taaatcatgt ggttctcgga
     2521 caatacctgg gccaggctgc gcgcgatgcc gaacatgccc agcgtcacga tgaacgacga
     2581 cagccgcacg taggcgatca ggaggccgtt gaccagcccg caaagcagcg cggtcagcag
     2641 gccggcggca attcccgccc acagcgaatg tcctgcgttg agcaccaggc cggtgacgat
     2701 tcccgatagc ccgacgacgg aaccgaccga aagatcgatg cccccggtga tgatgaccgc
     2761 ggtcatgccc atcgcgatga tgccgatgaa agcgaagttg cgcgtgacgt tgaagaagtt
     2821 gtcctctgta aaaaatacgt tcgatatctg cgacatcacc acgcagatca tcgcgagtgc
     2881 gaccgtcacc cagaacggct ggctgcggac catcgtccgc agcggactgt tctcccttct
     2941 ttgcgcgatt tcttccgacg agacgctcat tgtagccctc gccgtcaggc ggtttcgatg
     3001 gcgcccgtga tcaggccggt gacctcctcc ggggaagtct gcccgatcgg tttgtcggcg
     3061 acgactttgc cccggcgcat caccgagacg tgatcgcaca ccgcgaacac gtccggcatg
     3121 cgatggctga tcaggattac ggcgacgttc tggtcgcgca gccgccgtat caggtccagc
     3181 acctcggcga cctggcgtac gctgattgcc gcggtcggct catccatcag cacgatgtcg
     3241 ggatccgaca gacgcgtgcg tgcgatcgcg accgcctgcc gctggccgcc ggacatttgt
     3301 tttacgaaat cccgcgggcg cgtttccgat ttcagctcgg cgaagtggcg cgcggcttcg
     3361 cgaaacatgg ctgcgtaatg gatgaagttg aatggcccta tccggcgatg cgcctcgcgg
     3421 ccgaggaaga cgttctccgc ggcggtgagg ttgtcgcaca gcgccaggtc ctggtagacg
     3481 acttcgacgc ctttcttgcg cgcgtcgacg gggttatgga actcgactct ctcgcctttc
     3541 agccagatct cgccgctcga aggcaggaag ttgccggcca tgaccttgat cagcgtggat
     3601 ttgcccgcgc cgttgtcgcc catcaggccg agcacctcgc cggcgtcgat cttcaggtcg
     3661 acctcggtca gcgcctggat ggcgccgaac ttcttggaaa taccacgcag ctcgaacagg
     3721 cgcacggttc tcctctcaat ccgggcggat gatgcctttt ttcaggatca ggttgccgta
     3781 gagccggcgc tcgcccgtgg cgactaccgc gaaacagttc tttacgcgct cgtagaatgc
     3841 aaagcgctcg atgccttcga gcttcgccat cttcggttcg tatttgcgca g
//
LOCUS       DASQOP010000025         3017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1218655, whole genome shotgun sequence.
ACCESSION   DASQOP010000025 DASQOP010000000
VERSION     DASQOP010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218655"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(461..943)
                     /locus_tag="VE030_00815"
     CDS             complement(461..943)
                     /locus_tag="VE030_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF21987.1"
                     /translation="MLALGLLLNFAGIGLFCWLIFTLAVYALPFFVGLTVFMMALHGG
                     AGILGAPLVGIAACGMTLAIGQTAFAMTRSVIIRAIIAALFAVPATLAGYQVVFATSQ
                     IGVPSLVWREIFACLGAVSIGGTTWMRLTVFTETRPLDSDRAFGSDPQPVLTTAAHQK
                     "
     gene            complement(1082..2005)
                     /locus_tag="VE030_00820"
     CDS             complement(1082..2005)
                     /locus_tag="VE030_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2493 domain-containing protein"
                     /protein_id="HZF21988.1"
                     /translation="MTDYDDIEPAHGASATEHVLSELQLFGYRPFDDQPDPRPLPEGK
                     MITGAVADIFDALVATLSDTRLEPDLEDLLWSTVNLFHRATDRIGRQLDDNEQAQRKS
                     QREQNGSEVQSVELERLTAEGITLIERRNCLELFRDQAIERFEVHTGSSWRPRSGSLV
                     NHRTLTAAMIDSREFIAAKRRAETEVILPAGPKVALTGGLNFNDHHLIWDRLDKVHAK
                     HPDMVLIHGGSPKGAELIASKWATTRKVPQIAFKPDWTKHAKAAPFRRNDAMLELLPI
                     GVMHFPGTGIQDNLADKAKRLGISVWKFGGA"
     gene            complement(2351..>3017)
                     /locus_tag="VE030_00825"
     CDS             complement(2351..>3017)
                     /locus_tag="VE030_00825"
                     /inference="COORDINATES: protein motif:HMM:NF024755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HZF21989.1"
                     /translation="IEGTIVETYLHRRGIAHVYHGSSLRFHPRCYYRPDEHLPTETWP
                     AMIACVTDLHGRITGVHRTWLDPDGFDRIRLGKAPIDTPRRAMGDLLGNAVRFGLADD
                     DMLVAGEGIETMLSLRCVLPSMPMAAALSANHLAAMLLSSGLRRLYIARDADAAGDAV
                     QATLMQRSGAAGIETIALSPRLGDFNEDLHVFGLDALRAALRIQLAPEDVDRFLLPST
                     ALG"
BASE COUNT          612 a          860 c         1001 g          544 t
ORIGIN      
        1 tccgggccgc agcgaagcgg aggacgccgt cgtagagact ttcttgcctc gcgaggaatg
       61 ggcgtagccc aggggaagaa agtctcaagc gacggcgttg cggctcagac gatcgaggcg
      121 cagccggtct tcggccagat cgagccatca aagaggccac gtgcgtcgcg tgctgaacca
      181 caagtccgag agaagatggc gggcttcggt tcgcctaggc aagaggtcga aacagttcgg
      241 cgtaggaaaa gaaacgaggg aaggcaaaag gcgatgtcgt cagcgcaagg ccgatcgcga
      301 acgaccggct ctagcgccgc atctatgtgc gctgcacgta atcagcgcca tcgcatcgaa
      361 gtgctctgag ggaggtcgcg actatctctt gctcaagctc gcggatccgt tgcagcgctt
      421 cgattcagcc aacttgttcg acgaagcgga tggtgcagga tcacttctga tgggctgcgg
      481 tagtgagaac cggttgagga tcgctcccaa atgccctgtc cgaatcgagc gggcgggtct
      541 ccgtgaagac ggtcaatcgc atccacgtgg tgccgccaat agagaccgcg ccgagacagg
      601 cgaagatctc acgccatacc aatgacggca cgccgatttg cgacgtcgca aatacgacct
      661 ggtagccggc aagtgtcgcc gggacagcaa acagggcggc aatgatggca cggataatca
      721 cggagcgtgt cattgcgaag gctgtttgac cgatcgcaag agtcatcccg caggcggcaa
      781 tgccaacaag cggcgcaccg aggatgccgg caccgccgtg caatgccatc atgaagacgg
      841 tgagtccgac aaaaaacggc aaggcgtata ccgccagcgt gaagatcagc cagcagaaca
      901 agccaatgcc agcgaagttc agcagaagtc caagggcgag catggtggtg gctccatatg
      961 caaaggtatg gttgcgcctt ccaccaccac cacggcgcag ttttggatat gcagtagaaa
     1021 cgaggcggag ttgcggcgat gcaactccac ctcggtggtg tggaagatgg ccggtcggcg
     1081 ctcacgcgcc gccgaatttc caaacggaga tgccgaggcg tttcgccttg tcggcgaggt
     1141 tgtcctggat tcccgtgccc gggaaatgca tgacgccgat cggcagtagt tctagcatgg
     1201 cgtcgttgcg tctgaacggt gctgccttag catgcttcgt ccagtccggc ttgaaagcga
     1261 tctgcggcac cttgcgggtg gtcgcccatt tggaggcgat cagctcggcg ccttttggtg
     1321 aaccgccatg gatgaggacc atgtcgggat gcttggcgtg gaccttgtcg aggcgatccc
     1381 agatcaagtg gtggtcattg aagttgagac cgcccgtaag tgcgaccttt ggtccagccg
     1441 gcaaaatgac ctcggtctcg gcgcggcgct tggctgcgat gaactcccga gagtcgatca
     1501 tggccgcagt gagcgtgcga tggttgacaa gcgatcccga ccgggggcgc caggacgaac
     1561 cagtgtggac ctcaaagcgt tcgatggcct gatcgcggaa gagttcgaga cagttacgac
     1621 gttcgatcaa agtgatgcct tcggccgtca ggcgctcaag ttcgaccgac tgcacctccg
     1681 acccgttctg ctcgcgctgg cttttgcgtt gcgcctgttc gttgtcgtcg agctggcgtc
     1741 cgatgcggtc ggtggcacga tggaagaggt taaccgttga ccagagcaag tcctcaaggt
     1801 ccggttcgag ccgggtgtcg ctcagagtgg cgaccagggc gtcgaagatg tcggccaccg
     1861 caccagtgat catcttgcct tcgggaagcg gtctgggatc gggctgatcg tcgaatggac
     1921 ggtagccaaa gagctgcaat tcgctgagca cgtgctcggt cgcagatgcg ccgtgtgctg
     1981 gctcgatgtc gtcgtagtcg gtcatgagcg aagtcctttg ccggttcggc cgcgcccatc
     2041 gcggccttcg tggcgatcac tcagacggca ggcgggacgg gccagaaccc gaaagcggca
     2101 gcggagggcc gtagcgccag cggaggatgg cagaggtgcg gctattttgc ttcgcgatgc
     2161 aaagcgcgcc atcgccctat gtgtaatgtg cggcctttcg gcggcgcgcg gcggaaaata
     2221 gccgcacgtc tgccattgtc ggcccgggtc gcttgccgtc cgatcgccct ctagaaggcc
     2281 gtggtcgcgg ccctcgccgg cattggatgc ccgcgaccga tctgctgacg tcgatggaag
     2341 gcaaacgcag ctatccgagc gctgtcgagg gcaagaggaa gcggtcgacg tcctcaggtg
     2401 cgagctgtat ccgcaacgct gcgcgtaggg catcgagacc gaagacgtgc agatcttcgt
     2461 tgaagtcgcc cagtcgtggc gacagggcaa tcgtctcgat gccagcggct cctgagcgtt
     2521 gcatgagagt cgcctgcacg gcgtctcccg cggcatcggc atcgcgagcg atatagagtc
     2581 ggcgcaaacc cgacgacagc agcatggccg cgaggtgatt ggcagacaaa gcagccgcca
     2641 tgggcatgct cggtagcacg catcgcagcg acaacatggt ctcgatgcct tcgcccgcga
     2701 caagcatgtc gtcatcagcc aagccaaaac gaaccgcgtt gccgagcagg tcacccatcg
     2761 cacgtcgcgg cgtgtcgatt ggagccttgc cgagccgaat ccggtcaaat ccatcaggat
     2821 cgagccatgt gcggtgcacg cccgtgatcc ggccgtggag gtcggtgacg caggcgatca
     2881 tcgccggcca ggtctcggtc ggaaggtgct cgtcaggtcg atagtagcaa cgcggatgga
     2941 agcgcaggct gctaccatgg tacacatggg ctatgcctcg gcggtgcagg taagtctcaa
     3001 cgatggtccc ctcaatc
//
LOCUS       DASQOP010000026        13356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2225643, whole genome shotgun sequence.
ACCESSION   DASQOP010000026 DASQOP010000000
VERSION     DASQOP010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13356
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2225643"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VE030_00830"
     CDS             complement(<1..308)
                     /locus_tag="VE030_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF21990.1"
                     /translation="MHRSAIILPRAVAWISLLACMSVAYAGVFGDGDRVMLEFGPYVA
                     HRVDNTGHNQWPNLVGVEWESGSHWLVGAAAFKNSYYQHAAYAYAGRRWFLDRVDPNL
                     "
     gene            complement(664..1467)
                     /locus_tag="VE030_00835"
     CDS             complement(664..1467)
                     /locus_tag="VE030_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF21991.1"
                     /translation="MQARTKLALRISQRCFLLLLCLPLPLPAQTQPAASTAAEATLNK
                     PTDFRSRVEIRNEYQDLKDGGYRNLVIPRFEYAVTPAVAFRIETPYIEYDPGTSGTVR
                     SGGFGDLLVRGAWRATQRDGFALILATEVIFDTAEDPRFGQGKTIVAPLVYAAIDLPK
                     VDSVFFPNIQHYFSVAGDESRADVSFTTLKPNLLTRWPNKIYTFLEPQFTIDWERDAK
                     VGLTVELEVGKILSKNIAAWARPGIGVINRNELPQVYNWNLEVGMRYIF"
     gene            complement(1560..2834)
                     /locus_tag="VE030_00840"
     CDS             complement(1560..2834)
                     /locus_tag="VE030_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZF21992.1"
                     /translation="MAKVLFKNVNVLDCSGAAAYAGQVLVEGNRIKTVAPQGSKIAAE
                     GAQVVDGGGATLMPGLVEAHSHMTFLDTPDLESLGFVPPEEHMLRTVKNAKKMLDQGF
                     TACNSAASAKARLDVVLRNAINAGDFPGPRTLAASPELATTAGLGDVRLMHMRRETFA
                     IICDGAEEFRKTAREMVREGVDTLKLNPSGDEFIPHARAHHTVMTEAEIAAVCDVAHA
                     HGKRLAAHARSAESVKLCLKHGVAVIYHATLADDEACAALEKAKDWCFVAPTLGISYT
                     TLNEAAKWGITTQIAESLGIRRELDTACKNMKELKKRGVRVLPGGDYGFAWNPIGTNA
                     RDIEHFVNLLGFSPMDAILSATKLGGEIMMAGNEMGQIKPGYLADMILVDGNPVANVK
                     ILQDATKLLAIMKDGQFHKEPHTASQSQRQIA"
     gene            complement(2827..3927)
                     /locus_tag="VE030_00845"
     CDS             complement(2827..3927)
                     /locus_tag="VE030_00845"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZF21993.1"
                     /translation="MAHVRLEKVTKKFGSHTALSKLDLEIRNSEFFVLLGPTGAGKTT
                     TLRLIAGLESPNGGTISIDGTDVLNWSAAERDVALVFQYYSLYPHYTVRQNLEFPLKS
                     KVRRMSQQEIDARIAKAAKTLRIEHLLERKTDRLSGGEMQRVSIGRAIVREPRVFLMD
                     EPLSNLDAKLRELLRAELKDLQMKLGATFLYVTHDQVEAMSMGDKVGVLNQGRIIQVG
                     TPQQIYNQPHDTFVATFVGSPAMNLMQANVAGGRAVVVPGKLEIPVDGAGGGRALRVS
                     GAVTLGVRSEDIVVGANGTVVARVHGVENQGVEKVVTLRVDECLLKATVPARLELGID
                     TSVRFSFKQEKLQFFDAGSGINLFYGEEGKNG"
     gene            complement(3933..5042)
                     /locus_tag="VE030_00850"
     CDS             complement(3933..5042)
                     /locus_tag="VE030_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZF21994.1"
                     /translation="MADIVLRDVEKYFGDNYIIRKLNLEIRHREFVVLLGPSGCGKTT
                     TLRAIAGLEEIDTGDILIDGKPVQAMQAADRDIAFVFQLFALYPHLTAYENIAFPLRA
                     TNESRETVDRRVREAAKSLRIEHLLDKRPGALSGGDLQRVAIGRALVRRPKALLMDEP
                     IGALDAKLREEMRTELKRLHIENEATTVYVTHDQVEAMSMADRIAIMNDGVLQQVGTP
                     TEVYQHPENLFVAQFIGSPIMNVVNGAVRPGGGDTQLILGDNDAQLSFPQKLYPLIVE
                     KKHAEDELAIGVRPEGVLVARQQTPGYVPVEAHIIEPLGPFDIVDLKFGAQMLRARTS
                     SGYVARAGDVVWAKLDEAQTHFFSTRSGESLHIRL"
     gene            complement(5099..6241)
                     /locus_tag="VE030_00855"
     CDS             complement(5099..6241)
                     /locus_tag="VE030_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZF21995.1"
                     /translation="MRLRMGMVGGGLDAMAGELHRRAAALDSRVELVAGALSSTPERS
                     RAAAKAWGLARGYGSWREMLDAEAKLPVTERIEFVTVATPNHLHVPVSVAFAERGFHV
                     ACDKPIGLSLEEVRHLCGVVEKSGIVFLLTHNYTGYPMVKQARHMIASGELGDIIKVM
                     VDYPQGWLLQPIEKLGHKGAAWRTDPSKSGAAGCLADIGSHAQHMMHTVTGLEIDELC
                     ADFGHLHGRKVDDDISILLRFRGGARGLLSASQVMAGEEGGPNFRIYGTRGALEWKQL
                     EPESLIYRQPDQPVRTFRRGESYLCDAAKRASRLPPGHPEGYYEAFGNLYRNFADAII
                     ARRQGTHAEEDFPTAIDGARTMAFIETALESNAAGGKWMRVKDHAH"
     gene            complement(6241..6432)
                     /locus_tag="VE030_00860"
     CDS             complement(6241..6432)
                     /locus_tag="VE030_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF21996.1"
                     /translation="MNLRELSARVLEPLSMYTMIFGIVALCQPWIEVLHRYGVTITLI
                     GLIGFSVFSKIKPLPEAER"
     gene            complement(6516..7475)
                     /locus_tag="VE030_00865"
     CDS             complement(6516..7475)
                     /locus_tag="VE030_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HZF21997.1"
                     /translation="MAAVTSKGHKFWNRALVGIVFVLLVIYLLPLYWISSTAFKPRSL
                     ATTVPPTVVFEPELTSFVKLFTSRVQMRGEVEPEVYQKAPWYEKRVFDSGERIIKDAN
                     GKLESSGYLSRFLNSIIIAVTSTFLAVAMGTLTAYGFSRFKLPGEQDWLFFILSTRML
                     PPVVVAIPMFLMYRTVGLVDTHIGLIILYTAFNLSFSVWLMKGFIDEIPIEYEEAALV
                     DGYTRMQAFFKIVLPQAATGIAATAVFCFITAWNEYAFALMMTNSKAQTAPPFIPSQL
                     GSGVTDWTVIAAGTFLFLVPVAIFTFVLRKHLLRGVTFGAIRK"
     gene            complement(7481..8374)
                     /locus_tag="VE030_00870"
     CDS             complement(7481..8374)
                     /locus_tag="VE030_00870"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HZF21998.1"
                     /translation="MLARLDPGSASARRGLSDIAIRNLFIIPTIVFLIVFNIFPLIYS
                     LGYSFTDFRASGSAPANFIGLQNYRELLADEYIWNNFTITAKYVLVSVGGQVLVGFGV
                     ALLLNRKIPAKGLVTTLLLLPMMMSMAVVGLFWQLLYSPSWGIINYVLGLEDFGWLSN
                     ADVALYAVALTDIWMWSPFVMLLSLAGLSAIPQHLYEAAAIDRATRWYTFTRITLPLV
                     TPLLLIAIIFRTMEAFKTFDLAFIMTSMESTELIAIRLYKMAFQEWQTGKSCAMAYVV
                     LIMVLAITNIYVKYLNQAKAR"
     gene            complement(8475..9788)
                     /locus_tag="VE030_00875"
     CDS             complement(8475..9788)
                     /locus_tag="VE030_00875"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZF21999.1"
                     /translation="MSKKKWSGLLAGAVFALACSSAPAAEKLTILWAQWDPANYLQEL
                     VKDYEKQTGVTVKVETTPWPDFQTKAFREFAAKGDAYDLVVGDSQWLGAGSTGGHYVE
                     LTDFFKKHGVDKSMAPATVEGYAEYPKGSKHYWAVPLEGDATGWSYRKDWFDDPKEKA
                     AFKKKYGYDLGVPKTWAHMRDIAEFFHRPSEKRYGIAIYTDNSYDALVMGYENVLFGY
                     GGDLGDYKTYKVKGIIDSKTAVDALKFYKELYKYTPPGWGKVFFLEDNQAITEGLASM
                     SMNFFAFFPALTNPATNKNAKVTGYFANPEGPTGKRFAALGGQGISIIKYSKKQDQAM
                     KFLEWFIKDDVQQKWAELGGYTCSAKVLQSENFRKATPYNQAFYETMFMVKDFWAVPE
                     YAELLDSMNKRLHPFIVGNKGSAEEALAGVANDWDKTFRKAGLLK"
     gene            complement(9924..10676)
                     /locus_tag="VE030_00880"
     CDS             complement(9924..10676)
                     /locus_tag="VE030_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZF22000.1"
                     /translation="MVVSGQEQRLTGRKGKVRDDKARADRGPRARMRKIMLDAGMLLM
                     QNGIIPSVSDVAEAAQVSRATAYRYFATQASLIQAAVDEALGPILAWKSESQDPEERV
                     TQLVTFAYPRMEFYEATLKAALRLALDQWARKHAGTLGDEAPMVRGHRIALLNSAVAP
                     LKGVLNRQGFDRLTQALSLIFGTEAFVVLKDIWGLDRKRAQEVALWTCHALIQAALAE
                     ASTGKARAGTARRNGARSASALLKKGGRVKIK"
     gene            10866..12059
                     /locus_tag="VE030_00885"
     CDS             10866..12059
                     /locus_tag="VE030_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HZF22001.1"
                     /translation="MKRIYVAGTADTKGQELQYVKSLIENAGGPALLVDVGIKAPKVA
                     VDVAAAEIAAFHPKGAQFVLGSVDRGVAVEAMGEAFARYLGTRSDIAGIIGIGGGGGT
                     SIVTAGMRRLAVGLPKLMVSTLAAGDVSQFVGAGDITMMYSVTDVAGLNRISRVVLGN
                     AAHAIAGMATHKVPEAAAKPAIGLTMFGVTTSCVTQVVAQLEDQYDCLVFHATGTGGR
                     SMERLADSGMLKAVIDVTTTEICDHLVGGVLSAGPDRLGAIARSRVPYVGSVGALDMV
                     NFWAMDTVPERFRLRKLYKHNPNVTLMRTTASECRSIGTWIAERLNACEGPVHFLIPE
                     QGVSALDAPDQAFYDPEADDALFQSLERTMRQTPVRRLTRLPCHINDPQFSAALAEAF
                     REIIR"
     gene            12073..12903
                     /locus_tag="VE030_00890"
     CDS             12073..12903
                     /locus_tag="VE030_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate hydrolase family protein"
                     /protein_id="HZF22002.1"
                     /translation="MPAIGRRILIEKFQDMVRKRVPIVGGGAGTGLSAKCEEAGGIDL
                     IIIYNSGRYRMAGRGSAAGLLAYGNANQIVKEMAFEVLPVVKKTPVLAGVNGTDPFVL
                     MPQFLAELKYLGFSGVQNFPTIGLFDGVMRASFEETGMGYGLEVDMIGLAHEQDLLTT
                     PYVFNTGEAKDMTRAGADVVVAHMGVTVGGNVGASSARSLDTCVKEIDAIAEAAMKVR
                     KDVIVLCHGGPIASPENAGYILKRTKNCHGFFGASSMERLPTEVALTDQTRKFKRIKF
                     "
     gene            13153..>13356
                     /locus_tag="VE030_00895"
     CDS             13153..>13356
                     /locus_tag="VE030_00895"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZF22003.1"
                     /translation="MASVYRSAVIDAPAAKVWALVRDFNGLPKWHPAIGKSAIEGGLP
                     GDRVGCVRRFTLKKDGGLLREKLL"
BASE COUNT         2447 a         4190 c         4089 g         2630 t
ORIGIN      
        1 tagagattcg ggtcgactcg atcgaggaac cagcgcctgc ccgcataggc gtacgcggcg
       61 tgctggtagt aagagttctt gaacgcggcg gcaccgacca gccaatgcga acccgattcc
      121 cattccacac cgacaagatt cggccattga ttatgtccgg tattgtcgac ccggtgcgct
      181 acgtagggtc cgaactccag catcacgcga tcgccgtcgc caaacacacc ggcatacgcc
      241 accgacatgc aggcaaggag ggatatccac gcaaccgcgc gcggcaaaat gattgcagac
      301 cgatgcataa ggggatctgg ctgggcagtc gacttggtat tccaattaag agagaagctt
      361 cgattttccg ctagccatac cgtcgatttt actggcgttg tgctttggag tccgatttca
      421 acgggagggg atcgggccaa tgacgaagcg atttccggaa gagcagatca tcgggtttct
      481 gcgggattct tacgtaggcc cttcaagcgt actttttcga gcaccggaag caaagatggc
      541 ctgatccccc gcttctcttt tctgcctttc agttttcctt tgcgtccttt gcgtcaacgc
      601 ttttaatgcc ggccatcggc cggcttgcac tttttacccc ttactccttg cccctcgccc
      661 ctactaaaaa atataccgca tccccacttc gaggttccag ttgtagacct gaggcagttc
      721 atttctgttg atcaccccga tccccggtcg ggcccaggcg gcgatgttct tggaaaggat
      781 tttcccgacc tcgagttcga ccgtcagtcc gaccttggca tcgcgttccc agtcgatggt
      841 gaactgcggt tcgaggaaag tgtagatctt attgggccag cgggtcagca ggttgggttt
      901 gagagtggtg aagctcacat ccgcgcgact ctcatcgccg gcaaccgaga agtaatgctg
      961 gatgttgggg aagaacaccg agtcgacctt gggcagatcg atggcagcgt aaacgagcgg
     1021 tgcaacgatg gtcttcccct gcccgaatcg aggatcttcg gccgtatcga agatcacttc
     1081 cgtcgccagg atcaaggcaa agccgtcgcg ttgcgtcgcg cgccaggcgc cgcgcacgag
     1141 cagatcgccg aacccgccgg agcgtacggt gccggaagtg cccgggtcgt attcgatgta
     1201 aggcgtctcg atccggaacg cgacagccgg ggtcaccgcg tactcgaaac gaggaatgac
     1261 cagattccgg tagccgccgt ctttgaggtc ctgatactcg ttgcgtatct ctacgcgaga
     1321 gcggaagtcg gtgggtttat tgagcgtcgc ttcggccgcg gttgacgcag ccggctgggt
     1381 ctgtgcgggc aacggcagcg gcaggcacag caagagcaga aaacagcgct gactgatgcg
     1441 aagggcaagc ttggttcggg cctgcatgct tttttgtacc tccccaaaaa aaagcgcccg
     1501 caagcgggcg cgatccctac tacaaggttt attgttgttt ctttccttct tcttattcct
     1561 tacgctatct gccgctgcga ctgcgaggcg gtgtgcggct ccttgtggaa ttgcccgtct
     1621 ttcatgatcg ccagcagctt ggtggcgtcc tgcaggattt tcacgttggc gaccggattg
     1681 ccgtccacca ggatcatatc agcaagatag cccggcttga tctgtcccat ctcgttgccg
     1741 gccatcatga tctcgccgcc gagcttggtc gcggacagga tcgcgtccat gggcgagaaa
     1801 cccagcaggt tcacgaagtg ctcgatatcg cgggcgttgg tgccgatcgg attccaggcg
     1861 aatccgtagt ctccgcccgg caggacgcgc acgccgcgct tcttcagttc cttcatgttc
     1921 ttgcaggccg tatcgagttc gcggcggatg cccaaactct cggcgatctg cgtcgtgatg
     1981 ccccacttcg ccgcttcgtt gagcgtggtg tagctgatgc ccaaggtcgg cgccacgaaa
     2041 caccagtcct ttgctttctc cagcgcggcg caggcttcgt cgtccgccag cgtagcgtgg
     2101 tagatcaccg cgacgccgtg cttcaggcac agcttcaccg actcggcgct gcgcgcgtgc
     2161 gcggccaggc gtttgccgtg ggcatgcgcc acgtcgcaga ccgccgcgat ttccgcttcg
     2221 gtcatcaccg tatggtgcgc gcgtgcatgc gggatgaact cgtcgcccga cgggttgagc
     2281 ttcagcgtgt ccacgccttc acgcaccatc tcgcgggcgg tcttgcggaa ttcttcggcg
     2341 ccgtcgcaga tgatcgcgaa agtctcgcga cgcatgtgca tcagccgcac gtcgcccagt
     2401 ccggccgtcg tggcgagttc ggggctggcg gccagcgtcc tcggtcccgg gaaatcgccg
     2461 gcgttgatcg cgttcctcaa caccacgtcg agccgcgctt tcgccgaggc cgcgctgttg
     2521 cacgcggtga agccctggtc gagcattttc ttcgcgttct tcaccgtgcg gagcatgtgc
     2581 tcctcgggcg gaacgaagcc cagtgattcc aggtccggag tgtcgaggaa ggtcatgtgc
     2641 gagtgtgctt cgaccaggcc cggcatcagg gtcgccccgc cgccatccac cacttgcgcg
     2701 ccttcggcgg cgattttcga accctgcggc gcaaccgtct tgatgcgatt gccttcgacc
     2761 agcacctggc ccgcgtacgc cgctgcgccg ctgcaatcca ggacattcac attcttgaac
     2821 agaaccttag ccatttttgc cctcctcacc gtagaacagg ttgatgccgc tgccggcgtc
     2881 gaaaaactgc agcttttcct gtttgaatga aaacctcacg ctggtatcga tgcccagttc
     2941 caggcgcgcc ggaacggtgg ccttgagcag gcactcgtcc acccgcaggg tcaccacctt
     3001 ctcaaccccc tgattctcca ccccatggac ccgggctaca actgttccgt tggcgccgac
     3061 tacgatgtcc tcggagcgca cgcccagcgt gaccgccccg gagacccgca gcgcccgccc
     3121 gccgcctgcc ccgtcgaccg gtatttcgag ctttccgggc accaccaccg cgcgtccccc
     3181 ggccacgttg gcttgcatca ggttcatcgc cggggagccg acgaaagtgg caacgaaagt
     3241 gtcgtgcggc tgattgtaga tctgttgcgg ggtgccgacc tggatgatgc ggccctggtt
     3301 aagcacgcct accttgtcgc ccatggacat cgcttccacc tggtcgtggg tcacgtacag
     3361 gaaagtggcg ccgagcttca tctgcaggtc cttcagctcg gcgcgcagga gctcgcgcag
     3421 cttggcgtcc aggttcgaca gcggctcgtc catcagaaac acccgcggct cacgcacgat
     3481 agcccggccg attgagacgc gctgcatctc gccgccagag agccggtccg tcttgcgctc
     3541 caggagatgc tcgatgcgca gggtcttcgc ggccttggcg atgcgcgcat cgatctcctg
     3601 ctgcgacatg cgccggacct tcgatttaag cggaaattcc aggttctgcc gcaccgtgta
     3661 atgcggatac agcgagtagt actggaacac caacgccacg tcgcgctccg ccgcgctcca
     3721 gttcagcaca tccgttccgt cgatcgaaat ggtgccgccg ttcgggcttt cgagcccggc
     3781 gatcagccgc agcgtggtgg tcttgcccgc gccggtcggc cccagcagca cgaagaactc
     3841 gctgttgcgg atttccaggt cgagcttgct cagcgccgtg tggctgccga acttcttggt
     3901 caccttttcg agtctgacgt gagccatttt ccttacagcc gtatgtgcag cgattccccg
     3961 ctgcgggtgc tgaagaaatg ggtctgcgcc tcgtcgagtt tcgcccacac cacgtcgccg
     4021 gccctggcga cgtaacccga ggaggtgcgg gcgcgcagca tctgcgcgcc aaacttcaag
     4081 tcgacgatgt cgaaggggcc gagcggctcg atgatgtggg cctccaccgg gacatacccg
     4141 ggagtctgct gtcgcgccac cagcacgccc tcggggcgca cgccgatcgc cagttcgtcc
     4201 tccgcatgct ttttctccac gatcagggga tagagcttct gcgggaaact cagttgcgcg
     4261 tcgttgtcgc ccaggatcag ctgggtatcg ccgccgcccg ggcgcaccgc gccgttcacc
     4321 acgttcatga tcgggctgcc gatgaactgg gcgacgaaca ggttttccgg gtgttggtac
     4381 acctcggtgg gcgtgcctac ctgctgcagc acgccatcgt tcatgatcgc gatccggtca
     4441 gccatcgaca tggcttccac ctggtcgtgg gtgacgtaca cggtggtggc ttcgttctcg
     4501 atgtgcaggc gcttcagctc ggtgcgcatc tcctcacgca gcttggcatc cagcgcgccg
     4561 atcggctcgt ccatcagcag cgccttgggc cggcgcacta gcgctcggcc gatggccacg
     4621 cgctgcagat cgcctccgga cagcgcacca ggccgcttgt ccagaagatg ctcgatcctt
     4681 agcgacttgg ccgcttcgcg cacgcgcctg tccaccgttt cccggctctc gttggtggcg
     4741 cgcaatggga aggcaatgtt ctcgtacgcg gtcagatgcg ggtacaacgc gaataactgg
     4801 aacacgaagg cgatgtcccg atccgccgcc tgcatcgcct gcaccggctt gccgtcgatc
     4861 aagatgtctc cggtgtcgat ttcctcgaga ccggcgatcg cccggagcgt ggtggtcttg
     4921 ccgcagccgg acggcccgag cagcaccacg aattcgcggt gccggatctc caggttgagc
     4981 ttcctgatga tgtagttgtc gccgaagtac ttctcgacgt ctctcaagac aatatcggcc
     5041 atgaagaaag gtgatgggtg attgagtgat gtgcgatggg tgatgggtga tgggtgcgtt
     5101 agtgcgcgtg atccttgacg cgcatccact tgccgccggc ggcattgctc tccagcgcgg
     5161 tctcgatgaa ggccatggtt cgcgcgccgt cgatcgccgt ggggaaatct tcttcggcgt
     5221 gggtaccctg gcggcgggcg atgatcgcat cggcgaagtt ccggtacaga ttgccgaacg
     5281 cttcgtaata gccctccggg tggccgggcg ggaggcggct ggcgcgcttc gccgcgtcgc
     5341 acagatacga ctcgccgcgt cgaaacgtgc gcacgggctg gtccggctgc cggtaaatca
     5401 gcgattcggg ttccagttgt ttccactcga gcgcgccccg ggtaccgtag atgcggaaat
     5461 tcgggccgcc ttcctctccc gccatgacct gggacgccga cagcaggccc cgcgccccgc
     5521 cgcggaagcg cagcaggatg ctgatgtcgt cgtcgacctt gcggccgtgc aggtggccaa
     5581 agtccgcgca caactcgtcg atctccagcc cggtcaccgt gtgcatcatg tgctgggcat
     5641 gcgatccgat gtcggcaagg cagcccgccg cgccggactt cgacggatcc gtgcgccacg
     5701 cggcaccctt gtggcccagt ttctcgatcg gctgcagcag ccagccctgc gggtagtcga
     5761 ccattacttt gatgatgtcc cccagttcgc cgctggcgat catgtggcgc gcctgcttga
     5821 ccatcgggta gccggtgtag ttatgggtaa gcaggaacac gatgccgctt ttctccacga
     5881 cgccacataa atgcctcacc tcttccagtg agaggccgat tggtttgtcg caggccacgt
     5941 gaaatccgcg ttcggcaaat gccaccgata ccggcacgtg caaatggttg ggtgtggcta
     6001 ccgtgacgaa ttcgatgcgt tccgtgaccg gaagcttcgc ctccgcgtcc agcatttccc
     6061 gccaactgcc gtagccgcgc gccaggcccc aggctttcgc ggcggcccgc gaccgttcgg
     6121 gagtcgaaga caacgcgccc gccaccaatt ccacgcggct gtccagcgcg gcggcacgac
     6181 ggtgcaattc gcccgccatt gcgtccagac cgccgccgac catgcccatt ctcagccgca
     6241 ttaccgctcc gcttccggca agggcttgat cttggagaac accgagaagc caatcagccc
     6301 gatcagcgtg atggtgacgc cgtagcgatg cagtacctcg atccagggtt ggcacagcgc
     6361 cacgatgccg aagatcatgg tgtacatgga gagtggttcc agtacgcgcg cgctgagttc
     6421 ccgaaggttc atcatacatt tcctcaatat ggccttggct ggtcgtaacg gcgtgacggt
     6481 cgtgacggaa acccctagcc cctagtccct agcccctatt ttctgatggc cccgaaggtc
     6541 acaccgcgca acaagtgctt gcgcaggacg aaggtgaaaa tcgccaccgg taccaggaac
     6601 aggaaggtcc cggcggcgat cacggtccag tcggtcacgc cggagccgag ctgcgacggg
     6661 atgaacggcg gtgccgtctg cgctttcgag ttggtcatca tcagcgcaaa ggcgtattcg
     6721 ttccatgcgg tgatgaaaca gaacaccgcc gtcgccgcaa tcccggtggc cgcctgcggc
     6781 agcacgatct tgaaaaacgc ctgcatgcga gtgtagccgt ctaccagtgc ggcttcctcg
     6841 tattcgatcg ggatttcgtc gatgaatccc ttcatcagcc agaccgagaa cgacaggttg
     6901 aacgcggtat aaagaatgat gagcccgatg tgggtatcca ccaggccgac agtgcggtac
     6961 atgagaaaca tcgggatcgc caccaccacc ggcggcagca tgcgcgtgga gaggatgaag
     7021 aacagccagt cctgctcgcc cggcagcttg aagcgcgaga atccgtaggc ggtgagcgtt
     7081 cccatcgcca ccgcaaggaa ggtactggtc accgcgatga tgatgctgtt gagaaagcgg
     7141 ctcaggtaac cggaactctc gagcttgccg ttggcgtcct tgatgatgcg ctcgccggag
     7201 tcgaacaccc gtttctcgta ccacggcgcc ttttggtaaa cctccggctc gacttcgccg
     7261 cgcatctgca cgcgcgaggt gaacagcttc acgaaggacg tcagctcggg ctcgaacacg
     7321 acggtgggcg gcacggtggt ggcgaggcta cgcggcttga acgcggtgga gctgatccag
     7381 tacagcggca gcagatagat gaccagcagc acgaagacga tcccgaccag cgcccggttc
     7441 cagaacttgt gccccttgga agttacagcc gccacggtct ttacctcgcc ttcgcctggt
     7501 tcaggtactt cacgtagata ttggtgatcg ccagcaccat gatcaggacg acgtaggcca
     7561 tggcgcagga cttgccggtc tgccactcct ggaatgccat cttgtaaagg cggatggcga
     7621 tcagttcggt ggattccatg ctggtcatga tgaaggcgag gtcgaaggtc ttgaacgcct
     7681 ccatggtgcg aaaaatgatc gcgatcagta gcagcggcgt cacaagcggc agcgtgatgc
     7741 gggtaaaggt gtaccagcgc gtcgcccggt cgatcgcggc ggcttcataa aggtgctgcg
     7801 ggattgccga cagcccggcc agcgacagca gcatcacgaa cggggaccac atccagatgt
     7861 cggtcagcgc gaccgcatag agtgcgacat cggcgttcga cagccatccg aaatcttcca
     7921 ggcccagcac gtaattgatg atcccccacg aggggctgta aagcagttgc cagaacagcc
     7981 cgaccacggc catggacatc atcatcggca gcagcagcaa tgtggtcacc aggcctttgg
     8041 ccggaatctt tcgattgagc agcagcgcca cgccgaagcc gaccagcacc tggcctccca
     8101 cggacaccag cacgtacttg gccgttatcg tgaagttgtt ccagatgtac tcgtcggcga
     8161 gcagttcgcg gtagttctgc agcccgatga agttggccgg cgccgacccg gatgcgcgga
     8221 aatcggtaaa cgagtagccg agcgagtaga tcagcggaaa gatgttgaag acgatcagaa
     8281 aaacgatcgt cggaatgatg aacaggttgc ggatggcgat atcgctcaag ccccgcctcg
     8341 ccgaagcgga gccaggatcg agcctggcca gcataaaatg ctctccctat gattctccgg
     8401 cagcctgtcg ttcccgcgat acctcaaacg ggaaacgggg aaggccggag ccttccccgc
     8461 caatcacgaa acgattactt cagcaggccc gccttgcgga aggtcttgtc ccagtcgttg
     8521 gcgacgccgg ccagcgcctc ttcggcgcta cccttgttgc cgacgatgaa gggatgcaag
     8581 cgcttgttca tcgaatcgag cagttccgcg tactccggca ccgcccagaa atccttgacc
     8641 atgaacatgg tctcgtagaa cgcctggttg tacggagtcg ccttgcggaa gttctccgac
     8701 tgcagcactt ttgcgctgca ggtgtagccg cccagttccg cccacttctg ctgcacgtcg
     8761 tccttgatga accactccag gaacttcatc gcctggtcct gcttcttcga gtacttgata
     8821 atcgagatgc cctgcccgcc gagtgccgcg aagcgttttc cggtggggcc ttcgggattg
     8881 gcgaagtagc cggtgacctt ggcgttcttg ttggtcgccg gattggtcaa cgccgggaag
     8941 aaagcgaaga agttcatgct catcgacgcc aggccttcgg taatggcctg gttgtcttcc
     9001 aggaagaaca ccttgcccca gccgggcggg gtgtacttgt acagctcctt gtagaacttc
     9061 agcgcgtcca ccgcggtctt gctgtcgatg atgcccttga ccttgtaggt cttgtaatcg
     9121 ccgagatcgc cgccgtagcc gaacagcacg ttctcgtaac ccatgaccag ggcatcgtag
     9181 ctgttgtcgg tgtagatggc gatgccatag cgcttctcgc tgggacgatg gaagaactcg
     9241 gctatgtcgc gcatgtgcgc ccaggtcttc ggcacgccca ggtcatagcc gtacttcttc
     9301 ttgaatgcgg ccttttcctt cgggtcatcg aaccagtctt tgcgatagga ccagccggtc
     9361 gcatcgcctt cgaggggaac ggcccagtag tgcttgctgc ccttggggta ctcggcatag
     9421 ccctcgacgg tcgccggcgc catgctcttg tccacgccat gcttcttgaa gaaatcggtg
     9481 agctccacgt agtgaccccc ggtggaaccc gcgcccagcc actgggaatc gccgaccacc
     9541 agatcgtagg catcgccctt ggcggcgaat tcgcggaacg ccttggtctg gaaatcgggc
     9601 catggcgtgg tttcgacctt gaccgttacg ccggtttgct tttcgtagtc cttgaccagt
     9661 tcctgcaggt agttcgccgg gtcccactgg gcccagagga tggtcagttt ttccgctgcc
     9721 ggcgccgaac tgcaggccag agcgaaaaca gcgccggcca gcaggccgga ccatttcttc
     9781 ttgctcattt gattctcctc catagtgcgt tggacattct tttgcatccg gccgtactgc
     9841 tcgagccgcc cggttgcacc ctcgaaacat tcgcggtctt gccgccgcgt tcgaatacac
     9901 ttcccgactc ccgacgccga gtgctacttt atctttacac gacccccttt tttcagcagc
     9961 gcactggcgc tgcgggcgcc gttcctgcgc gccgtgccgg cacgcgcttt ccccgtggat
    10021 gcttcggcga gtgccgcctg gatcagcgcg tggcaggtcc ataacgctac ttcctgcgca
    10081 cgcttgcggt ccagacccca gatatccttc agcaccacga aagcttcggt gccgaaaatc
    10141 agcgacaacg cctgggtcag gcggtcgaac ccctggcggt tgagtacgcc cttcagcggc
    10201 gcgaccgcgg aattgagcaa cgcgatgcga tgcccgcgca ccatcggcgc ctcgtcgccg
    10261 agcgtgccgg cgtgcttgcg cgcccactgg tccagcgcga gccgcagcgc cgccttcagc
    10321 gtcgcctcgt agaattccat gcgcggatac gcgaatgtca ccagctgggt gacgcgctct
    10381 tccgggtcct gcgattccga tttccacgcc aggatcgggc ccagcgcctc gtccaccgcc
    10441 gcctggatca aagatgcctg ggtagcgaag taacggtatg ccgttgcccg cgacacctgg
    10501 gccgcttccg ccacatcgct caccgaggga atgatcccgt tctgcatcag gagcatgccc
    10561 gcgtccagca tgatcttgcg catgcgcgcg cgcggccccc ggtcggcccg cgccttgtcg
    10621 tcccgcacct tgcccttgcg gcccgtgaga cgctgttctt gacctgagac gaccatctca
    10681 ctacgatacc agcgtcttac aaaaatacaa ccgccctgcg gtttttccag cgccgcgctg
    10741 cgccgcagca ccgaagtaga ggcggaccat gagcttcagc gaggagattc gacgggccgc
    10801 cgcggccgcg cgccacaaac ccaaaaggcg tcgacgctaa ggacgcaaaa catacctata
    10861 gcgcaatgaa gcggatctac gtcgccggca ccgccgatac caagggccag gaactgcagt
    10921 acgtcaaatc gctgatcgag aatgcgggcg ggcccgcgct gctggtcgac gtcggcatca
    10981 aggcgccgaa ggttgcggtg gacgtggcgg cagccgagat tgcggccttt caccccaaag
    11041 gcgcgcaatt cgttctcggc agcgtcgacc gcggcgtggc ggtcgaggcg atgggcgaag
    11101 ccttcgcgcg ctatctaggc acccgaagcg atattgccgg catcatcggc atcggcggcg
    11161 gcggcggcac ctcgatcgtc acggcgggga tgcgcaggct ggcggtcggg ttgccgaagc
    11221 tgatggtatc cacgctcgcc gccggagacg tcagccagtt cgtgggcgcc ggcgacatca
    11281 ccatgatgta ctcggtgacc gacgtggccg ggctgaaccg gatcagccgg gtggtgctcg
    11341 gcaacgccgc gcacgcgatc gccggcatgg cgacgcataa agtgcccgag gcggccgcga
    11401 aacccgcgat cggtctcacc atgttcgggg tcaccacgag ctgcgtcacg caggtggttg
    11461 cgcagctgga ggaccagtac gactgcctgg tgtttcatgc caccggcacc ggcggccggt
    11521 cgatggaaag gctggcggac agcggaatgc tgaaagccgt gatcgacgtg accacgaccg
    11581 aaatctgcga ccacctggtc ggcggcgtgc tctcagcagg tcccgatcgc ctcggtgcga
    11641 ttgcgcgcag ccgtgtcccc tatgtcggtt cggtcggtgc cctggacatg gtgaacttct
    11701 gggcgatgga taccgtgccc gagcgctttc ggctgcgcaa gctttacaag cacaatccga
    11761 acgtcacgtt gatgcgcacc accgcctccg agtgccgctc gatcggcacc tggatcgcgg
    11821 aacggctcaa tgcttgcgag ggtccggtgc attttctgat ccccgagcaa ggcgtgtccg
    11881 cgctggatgc gccggaccag gccttttacg acccggaggc cgacgacgcg ctatttcagt
    11941 ctctggagag gacgatgcgg cagacgccgg tgcggcggct aacgcgcctg ccctgtcaca
    12001 tcaacgaccc gcagttttcc gcggcgctgg ccgaagcctt ccgcgaaatc atcagataga
    12061 gggatcacga gaatgcccgc aatcggaaga agaatactca tcgagaagtt tcaggacatg
    12121 gtgcgcaagc gcgtgccgat cgtcggcggc ggcgccggca ccggactctc ggcaaaatgc
    12181 gaagaagccg gcggcatcga cctgatcatc atctacaact ccgggcgcta ccgcatggcc
    12241 ggacgcggct cggcggcggg attgctcgcc tacggcaatg ccaaccagat cgtcaaggag
    12301 atggccttcg aggtgctgcc ggtggtgaag aagaccccgg tgctcgccgg cgtcaacggc
    12361 accgatccct tcgtgctgat gccgcagttc ctcgcggagt tgaaatacct ggggttctcg
    12421 ggcgtgcaaa acttcccgac catcggcctg ttcgacggcg tgatgcgcgc cagtttcgag
    12481 gaaaccggga tgggctacgg actggaagtg gacatgatcg ggctggcgca cgagcaggac
    12541 ctgctgacca cgccttatgt cttcaatacc ggcgaagcga aggacatgac ccgggccggc
    12601 gcggacgtcg tggtcgcgca catgggagtg accgtcggcg gcaacgttgg cgcttcctct
    12661 gccaggagcc tggacacctg cgtgaaggaa atcgacgcga tcgccgaggc ggccatgaag
    12721 gtgcgcaagg acgtgatcgt gctctgccat ggcggcccga tcgcctcgcc cgagaatgcc
    12781 ggttacatcc tcaagcgcac caaaaactgc cacggctttt tcggcgccag cagcatggaa
    12841 cgcctgccga ccgaagtcgc gctcaccgac cagacccgca agttcaagcg catcaagttc
    12901 tgagggacta gatcaacact aaccgcggag gacgcaaagg acgccaagga aggcaagagc
    12961 aaagaatatg ctcgggtgag aacaattcat tggatcatga tctatagcag cgaaagttgg
    13021 cggcacaggt tcgcggaaag gtgcatcgct tctatccccg aattgctttt cctcgttctt
    13081 gtttttcctt cgcgtccttt gcgtcctctg cggtgagagt ttgtttcgat tcggtacaag
    13141 ggggagaaaa gcatggcgag cgtatatcga agtgcggtga tcgatgcgcc ggcggccaag
    13201 gtctgggcgc tggtgcggga cttcaacgga ctgccgaagt ggcacccggc gatcggaaaa
    13261 tcggctatcg aaggcgggct gccgggagac cgcgtcggct gcgtgcgccg ctttaccctc
    13321 aagaaggatg gcgggttgct gcgcgaaaaa ctgctg
//
LOCUS       DASQOP010000027         4080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_11191, whole genome shotgun sequence.
ACCESSION   DASQOP010000027 DASQOP010000000
VERSION     DASQOP010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11191"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VE030_00900"
     CDS             <1..336
                     /locus_tag="VE030_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22004.1"
                     /translation="RPVRTALQILQEGVQTLFHLLLFSLLVLGFGGLLYKALRSGGWV
                     ESVLGRIWDQHPTVAIVTLLAALAGATWGTRMFDRLPMYGKRGDMLVYCCLALGLFFA
                     FKLVVTGSL"
     gene            692..937
                     /locus_tag="VE030_00905"
     CDS             692..937
                     /locus_tag="VE030_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22005.1"
                     /translation="MRMRTLRLLTGMAIVAVPGFAQEVQTPAGNAAAEPGSSARQIAA
                     PAVPGAASAKKEEAAGQPETKKQRIRYTFPPAAARRM"
     gene            complement(1131..1973)
                     /locus_tag="VE030_00910"
     CDS             complement(1131..1973)
                     /locus_tag="VE030_00910"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZF22006.1"
                     /translation="MRLCTIKRGGREEAAVVARAGIVPVAAINAHLGKAWPEELYALI
                     HQGISANLLADAEQTPLKLDPKAVRYGPLYRHPRKILGIGLNYRDHAADLNAPYPTEP
                     ASFMKCDNTIIGPGDTIELPPQSERVTAEAEIGVIIGRQCRFVSEEQAPAHVAGYCLI
                     LDMTAEDILQKNPRFLTRSKNFDTFFSFGPELITADEVPDVQKVKVGTYNNGRLQREN
                     VVANMAFPPSYLVSFHSHVATLYPGDIISTGTPGAVVIGDGDVAECRIEGLGVLCNPV
                     KRKT"
     gene            complement(2050..2883)
                     /locus_tag="VE030_00915"
     CDS             complement(2050..2883)
                     /locus_tag="VE030_00915"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZF22007.1"
                     /translation="MPADENHVVAFYNRHPISRDQILASLAAQRGQLAHLAPEELFPY
                     DQDHYGGLEAVDALADCAAIGPGKQVADFCAGLAGPARYLAHEYGATVTCIELNPGRA
                     PGAADLTRRVGLDRQVRIVRGDVTRTPLAGDTMDAVISQEALLHVPDKRTALAEAYRV
                     LKKGGRLAFTDWIEHRRLDASDSESMWRGIAAQTIQSFDSYDRMLRDIGFRIVSRKDL
                     TAEWGRILEQRFAMYRKLREETIALGTPSGDEEFYRSYARLVALVQARTLGGGRFAAE
                     K"
     gene            complement(3012..3087)
                     /locus_tag="VE030_00920"
     tRNA            complement(3012..3087)
                     /locus_tag="VE030_00920"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3052..3054),aa:Lys,seq:ctt)
     gene            3433..3723
                     /locus_tag="VE030_00925"
     CDS             3433..3723
                     /locus_tag="VE030_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22008.1"
                     /translation="MKSDFKAASVLERLSTSLTDAEIAYQAGQGIHEFIVRVGGMRHV
                     VDFSDDLMEKKNEMDLSVVILGIVERASTQSLPVHFMVRNDNFDKLMQALKH"
     gene            complement(3807..>4080)
                     /locus_tag="VE030_00930"
     CDS             complement(3807..>4080)
                     /locus_tag="VE030_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22009.1"
                     /translation="LHPKIALEIVKRYATTEKMQTRVMLAAKRSIQLLHNALLTGYQA
                     YSPSEASDGAVHADKRENDRRTQRLPLACPDRRFRERRGFIARKVA"
BASE COUNT          756 a         1250 c         1244 g          830 t
ORIGIN      
        1 cgcccggtcc gcaccgctct gcagatcctg caggaaggcg tgcaaaccct gtttcatctg
       61 ctgttgttct cgttgctggt gctcggcttc ggcgggctgt tgtacaaggc gctgcgctcc
      121 ggaggctggg tcgaaagcgt actggggcgc atctgggacc agcaccccac cgtcgcgatc
      181 gtgacgctgc tggcggcact ggcgggcgca acctggggta cgcgcatgtt cgatcgtctg
      241 ccgatgtacg gcaagcgtgg cgacatgctg gtttactgct gcctggcatt gggcctgttc
      301 ttcgccttca agctggtagt aaccggttcc ttgtaagtcc tcccttgccc ccgtccgcag
      361 acccacccct gcggacgggt ttggcgaggg attcctcctt ctgctgtcgt tctgcgacaa
      421 caccaatcct tgagcaagct caggaagtta gccggacact gctgatcttt gtcgcctgcg
      481 ggcgactgcg ccgcccccgg cagacctcct aattgactgt tttttctccg ctctcgcaaa
      541 tggcatgcga gttgcgggga tactgatcaa gttcatccgc aaccggatgc gtgaccactg
      601 gccacgaccg gccttgccat ccgggacttc tccgcggcgg tcgaggataa cgccggcgtc
      661 gccgcagccg agttttccaa ggaaggcgcc catgcgtatg cgaacgttac ggttgctgac
      721 aggcatggcc atcgttgccg tgcccggatt cgcgcaggag gtacagacac ctgcgggcaa
      781 cgcggctgcc gagcccggct ccagcgctcg ccagatcgct gctcctgcag tcccaggcgc
      841 tgccagcgcc aaaaaagagg aagcggccgg gcagcccgaa acgaagaagc aaagaatccg
      901 ttacaccttt cctcccgcag cagcgaggcg catgtaggtg cgcccgctgc aaatgaaaag
      961 gccgcggaaa accgcggcct tttttttgct ggtgtgccgt tacgacggtc gctgcatctc
     1021 gcaggccgcg cgtggatttt aggtcggcgt catcgttgcc gtactggctt tctggtccgt
     1081 cctttaggcg cgcggcacca acgcaactac atcccaacag cagtgatcgg ctacgttttt
     1141 ctcttgactg ggttgcaaag aacgccgagt ccctcgatcc ggcactcggc tacgtcgcca
     1201 tccccgatca ccacggctcc cggtgtgccg gtgctgatga tgtcgccggg atacagggtc
     1261 gcaacatgag agtggaagga cacgagatag gacggcggga acgccatgtt ggccaccacg
     1321 ttttcgcgtt gaagcctgcc gttgttgtac gttccgacct tcaccttctg cacatccggc
     1381 acctcgtcgg cagtgatcaa ctcggggccg aacgagaaga aggtgtcgaa attcttcgag
     1441 cgggtgagga agcgcggatt cttctgcagg atatcttcgg cggtcatgtc gaggatcagg
     1501 caatagccgg cgacgtgcgc cggcgcctgt tcctcggaca cgaagcggca ttgcctgccg
     1561 atgatcaccc cgatttcggc ttccgccgtg acccgctccg actgcggcgg cagttcgatc
     1621 gtgtctcccg ggccgatgat ggtgttgtcg cacttcatga acgacgcagg ctcggtcggg
     1681 tagggcgcat tcagatcggc agcgtgatcg cggtaattca gcccgatgcc gagaatcttg
     1741 cgcgggtgcc ggtacagcgg cccgtatcgc accgccttcg gatcgagttt cagcggcgtc
     1801 tgctccgcgt cggcgagcag gttcgcactg attccctggt gaatcagggc gtacagttcc
     1861 tccggccagg cctttcccag gtgggcgttg atcgccgcaa ccggaacgat gccggcgcgc
     1921 gccaccactg cggcttcttc gcgtccgccc ctcttgattg tgcacaggcg catgctgctg
     1981 tcccccgaac gtcaatgatt tttcgaaagt tgcgattata aaggctgaac aaacacgccc
     2041 cggctgccgc tacttttcgg cggcgaagcg gccgcctccg agggtgcgcg cctgcaccag
     2101 tgcaaccagg cgcgcgtagg accggtagaa ctcttcatca cccgagggcg tgccgagtgc
     2161 gatcgtttct tcgcgaagtt tccggtacat cgcgaagcgc tgctccagaa tccttcccca
     2221 ctcggcggtc aggtccttcc tggaaacaat gcgaaaaccg atgtcccgga gcatacggtc
     2281 gtaagaatcg aaactctgga tggtctgcgc cgcgatgccg cgccacatcg attcggaatc
     2341 gcttgcgtcg agcctgcggt gctcgatcca gtcggtaaac gcgagccggc cgcccttctt
     2401 caggacccga taagcctcgg ccagtgcggt gcgcttatcc ggcacgtgca gcaacgcttc
     2461 ctgactgatt actgcatcca tggtgtcgcc cgccagcggt gtccgcgtca catcgccgcg
     2521 cacaatccgc acctgccggt ccagcccgac gcgccgggtc aggtctgccg ccccgggtgc
     2581 gcgacctgga ttcagctcga tgcaggtcac tgtcgcaccg tactcatggg caagatagcg
     2641 cgccggcccg gccagcccgg cgcagaagtc ggcgacctgc ttgcccgggc cgatcgcggc
     2701 gcaatccgcc agcgcgtcaa ccgcctccag cccgccgtaa tggtcctggt cgtagggaaa
     2761 aagttcttcg ggagcaagat gcgcgagttg cccacgctgc gccgcgaggc tggcgaggat
     2821 ctgatcgcgt gagatgggat ggcggttgta aaaggcaact acatgatttt cgtctgctgg
     2881 catcgcctgc cacccggaag ccctctaaaa actggcggca agtctacacc tccggagcgg
     2941 gcattccgag atcttcgccc tgacgacgta aaacaaaagg gggcggcgat cgccgccccc
     3001 tgagggtctc ttggtgggcc gtgcaggatt tgaacctgcg accgttggat taagagtcca
     3061 ctgctctacc agctgagcta acagcccttt ttgaagggcc gaattctagc aaaaatccgg
     3121 ggcgacgagt cgcaggatga cgggcagatg gcgcgaacca aaataaggcg gacaatgatc
     3181 gaattctgcg aatatgggaa tttgccgaac ccgccatgcg agaccgcacc caccttccgc
     3241 aactgtttcg tgccgggaac gattgcagga acggttcctg cgtcacatcg tgcaacgacc
     3301 taaaccattg gttcgtcggc acctcgtgtc ccgggaacgt cgtcacagag tgtcagtcgc
     3361 aacccatttg tgtcattccg gagtgcgggc agtaataata ttgcgggcgc cggaagtgag
     3421 aaggagcatc gcttgaaatc agatttcaag gcagcgtccg tgcttgaacg gctcagtaca
     3481 tctctgaccg acgcggagat cgcctatcag gccggccagg gaattcacga atttatcgtc
     3541 cgtgtgggcg gtatgcgtca cgtcgtggac ttcagcgacg atttgatgga aaagaagaat
     3601 gaaatggatc tctctgtcgt gatcctgggc atcgtggagc gtgcctcgac tcaatcattg
     3661 cccgttcact tcatggtgcg taacgacaac ttcgataagc tcatgcaggc attgaagcac
     3721 tgattgtgca gtcgacaaag aaaaagggcc ggtagacgcc ggcccttttt ttgtttccct
     3781 gctgggtagc cggacctcga gcccggctac gccaccttgc gggcgatgaa gccgcggcgt
     3841 tcgcggaaac gcctgtcggg gcaggcgagc ggcagtcgct gcgtgcgccg gtcattctcg
     3901 cgcttgtccg catgaacggc tccgtcggaa gcctcagaag gggaataagc ctgatacccg
     3961 gtcaacaggg cattgtgcaa gagttgaatg ctgcgctttg cggcaagcat gacccgcgtc
     4021 tgcatcttct ccgtcgtggc gtagcgcttg acgatctcca gtgcgatctt gggatgcaga
//
LOCUS       DASQOP010000028        19747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_816694, whole genome shotgun sequence.
ACCESSION   DASQOP010000028 DASQOP010000000
VERSION     DASQOP010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 19747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19747
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816694"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1334
                     /locus_tag="VE030_00935"
     CDS             <1..1334
                     /locus_tag="VE030_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22010.1"
                     /translation="AFAFAADDAEIKKIREEIESIKDGYETRIRQLEQRLEKAEQSTA
                     TAKPAAPAQSAPASPNAFNPAISLILGGQFNNLQRDPGTYQIGGFIPGGDEIGPGNRS
                     FNLGESELTVSASIDPYFSGFFVMAVTPENAVSVEEGYVHSTGLVPGVTLKFGRFLSG
                     FGYLNEIHAHAWDFVDAPLVHQAFFGGQLKEEGLQARWLAPTPVFLEFGLETGRGANF
                     PGSERNKNGVNSGMIFAHVGDDVGISNSYRFGASYRVAHAQDRPYDDFDASGTTLVTN
                     AFTGDSKMWGVDLVWKWAPNGNTVDRNFKFQAEYLRRKEDGDLVFDTAGLTPAAGASG
                     AYRSSQSGWYMQGAYQFMARWRVGLRYEELDSGNTDVGLVNSGTLTAADFPRLAQNDP
                     KRTTAMIDFSPSEFSRFRLQFARDEARFNEADNQVFLQYIMSLGTHGAHKF"
     gene            1402..2307
                     /locus_tag="VE030_00940"
     CDS             1402..2307
                     /locus_tag="VE030_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HZF22011.1"
                     /translation="MNRWLTLAGAAALSLACSPVFAAVNVFACLPEWAALASELGGDK
                     VSVYQASNALQDPHRIEARPSLVARMRSADLAICTGAELEIGWLPVLLQTAGNQKVQP
                     GKPGFIAAAELVDRLEIPTRLDRAEGDIHPAGNPHIQLDPHNVAKVAAVVTQRLQQLD
                     PANAAYYDTRGKDFQARWQQAIGRWEKEAAPLKGLRIVPYHKNTVYLSHWLGMSEVMY
                     IEPKPGIPPSAGHLSELVVRLQAEPADAVIRTAYQDPKAASWLSEKTKIPVVELPYTV
                     GGTPAARDLFGLFDDTIARLNKVKK"
     gene            2309..3076
                     /locus_tag="VE030_00945"
     CDS             2309..3076
                     /locus_tag="VE030_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HZF22012.1"
                     /translation="MADLEFSILLPAFLAGLLVTATHVPLGIQVLNRGIVFIDIAIAQ
                     IAGVGVIAAHFFGLEDQAYAVQVAALGAALLGALFLNWTEKRWPTVQEAIIGSVFVLA
                     ATLEILLLAGNPHAGEYLKDMLVGQILWVGHHQLVVVAALTFIILAAWFGIGMARFGR
                     VGFYLLFGLAVTASVQMVGIYLVFSSLIIPALASRNATRLRLAKAYAVGALGYFFGLI
                     ASIVFDLPTGAIIVWAMAVVGIVFSAFERGRPSAAAA"
     gene            3179..4204
                     /locus_tag="VE030_00950"
     CDS             3179..4204
                     /locus_tag="VE030_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZF22013.1"
                     /translation="MSSANIEVSEIQPQLSRAEQEAAVQYANGHADAAIEILGEALEA
                     EKVAPLPAWLMLFDLFRIQGRWTDFENLSKRYTALFGRAAPDWLSDDILPEGLSAELR
                     PGGVAYVEIDGPLGAASAPDLARIRVAAARHPVVHIDLTKIQHVQQEGCALLSQELQF
                     LAGNGNGVLFSGAERIEKMLRQAVDTMSKGAAFWQLLLDLQRLQGNQKKFESIALEYA
                     LYVETDPPAWEPVLTPVLPCETVEEKRGEPRYRPELIHITGEMTGLKDPQLQVLQQFA
                     SDRQYVNINVARLQRIDFVCAGNLANVIAALKGKGKTVRVLHANSLVTTLLRLFKVDE
                     NAALVGK"
     gene            4252..4791
                     /gene="hslV"
                     /locus_tag="VE030_00955"
     CDS             4252..4791
                     /gene="hslV"
                     /locus_tag="VE030_00955"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HZF22014.1"
                     /translation="MSDSIHGTTIVSVRRGSRVALGGDGQVTFGNVVLKASARKVRRL
                     FHERILAGFAGGTADAFTLFERFEAKLEKHQGNLLRSAVELAKDWRTDRMLRRLEAML
                     AVADHETSLIVTGNGDVVEPELGLVAIGSGGPYAQAAARALLENTELPPQDIVKRSLT
                     IAGDICIYTNQNHVIEVLD"
     gene            4913..6235
                     /gene="hslU"
                     /locus_tag="VE030_00960"
     CDS             4913..6235
                     /gene="hslU"
                     /locus_tag="VE030_00960"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HZF22015.1"
                     /translation="MTPQEIVHELDKHIIGQDAAKRAVAIALRNRWRRQQVTEPLRQE
                     ITPKNILMIGPTGVGKTEIARRLAKLADAPFIKVEATKFTEVGYVGKDVDSIIRDLVE
                     TAIKQTREQETGKVRHRAEDQAEERILDVLLPPAREIGFQAEQEDRDSATRQKFRKKL
                     REGELDDKEIEIEVAAMHTQMEILAPPGMEDLTSQIQSMFQNLGGTRRKTRKLKIREA
                     MQLLIEEEAAKLVNDEELKLKALTNVEQNGIVFLDEIDKITSRQETSGAEVSRHGVQR
                     DLLPLVEGTTVSTKYGMIKTDHILFIGSGAFHLSRPSDLIPELQGRFPIRVELGSLSV
                     EDFEKILTATDACLTQQYQALLETEGVQLEFRQEGIRRLAEIAWQVNERTENIGARRL
                     YTVMEKLLEDVSFEADRHQGQKIVIDAARVDTRLKELAQSEDLARYVL"
     gene            6364..8922
                     /gene="glgP"
                     /locus_tag="VE030_00965"
     CDS             6364..8922
                     /gene="glgP"
                     /locus_tag="VE030_00965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HZF22016.1"
                     /translation="MPQGTTFTLEVNAKLPRRLARLEELANNLCYSWDRQARLLFSRL
                     DTLLWDEVGHNPKAFLKRVDEKKLVDAADDPVFLGNYNRVLSEFDTYHGEPMRRDGSE
                     WLRQSDLVAYFCAEFGFHESLPIYSGGLGILAGDHCKSASDMRLPFIGVGLLYRQGYF
                     HQTIDTEGNQNALYSDSDFEDLPVSPVLRDDGSEVRVKVDLPGRAVQVKVWQVKVGHV
                     ALYLLDTDLVENSHDDRDITHRLYGGDRTTRIEQEIVLGIGGVRALVELGLKPTAWHI
                     NEGHPAFLVLERVRMLVAQGLTFDAALEAVAANTVFTTHTPVPAGHDHFSADMMRDYF
                     SWLRKELGMSEEDLLALGRVDGNQEFNMTALAIRGSRFQNGVSRIHGSVSARICAPLW
                     PEVEPEENPLSYVTNGVHVPTFLAPEWSDQFDKFLGFDWSHHANNRAFWRRIDDMPDH
                     LFWSVRQSLKAQMLHLVRHRVSRQHFRNRGSEAHLDRLLRYADPVNPNVLTIGFARRF
                     ATYKRAALLFEDLDWLRRILGDEKRPVLFIFAGKAHPADVPGQDIIRRLTQMAHLPDF
                     EGKILLIEDYDLRLARRLVSGVDVWLNNPVYPLEASGTSGMKAGMNGVLNLSVLDGWW
                     DEGYDGKNGWAIKPVSESLDEARRNFEESRTLYELLQDQVIPLYYRRGDMGYSAEWLH
                     MAKRSIATLLPRFSSTRMVSEYLAKFYLPATRQGRRYAESNFEAARRLAEWKKHVRSS
                     WRKVGIRRLDMPKKRIPFGDNVRFEVGVDLDGLKPEDLRVELLVANQISRDNGTQATC
                     YQFQSEGVRTEKGEHRFVLELSPEHCGKLGYRIRMYPHHELLTHPFEMGMMRWL"
     gene            8970..9356
                     /locus_tag="VE030_00970"
     CDS             8970..9356
                     /locus_tag="VE030_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007733663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZF22017.1"
                     /translation="MPVRIDRLDHLVLTVKDIDATCAFYSRVLGMDARTFGDGRRSLH
                     FGAAKINLHQQGMEFEPKAAKPGPGSADVCLIAATPLAEVIRHLHECGVEIIEGPVQR
                     TGAIGPILSVYFRDPDGNLIEISNYG"
     gene            9586..10719
                     /locus_tag="VE030_00975"
     CDS             9586..10719
                     /locus_tag="VE030_00975"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZF22018.1"
                     /translation="MRVSLFGALATAVTASLIGLSAIAADPIRIGLVSEITGPNAEAG
                     GYTINGAKLAVEEINKKGGILGQQVELVVEDNQSTNPGTVLAISKLGGRKDIPAIIGP
                     IRSTQTQAASPTIAKLGIPTMIGGTDPSLTRVNNRWLFRCRPNDLYSSRVIADFGVNS
                     LKLKKWAVVHSTDAFGSGGSKALTDALMAYSVEPVLVQGYTNNSQDFTPVVLAIKKSG
                     ADIIGTYMTNSQDLGIFAKQLRQLGVNATWVGSASIVTDTAMKLAGEALYGTYGVADF
                     NPDANDEAKAFSKMYRAKYKLEPDVYSSWAFDAMNVIATALNGAKDTKPENIRNGIVG
                     LKNWKGVEGIYNFDEAGDGLRGYNIVKNDNGKVAFVKHINFEK"
     gene            10781..11662
                     /locus_tag="VE030_00980"
     CDS             10781..11662
                     /locus_tag="VE030_00980"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZF22019.1"
                     /translation="METLANNFQVLFQGLALGSIYALVALGFVLIIRATNVVNFAQGD
                     FAMLGAYMMVACLVGAGLPYWLSFVLALIGMGVFGILFNYGVYYPLRHRGFLPVIIST
                     LGASIFLQNTVLAIFGAQPKRLDNVIQGGVQIGSVFLDSQYLVILAVTMVMVAFQYFL
                     FEHTLVGKKLQATSQDKDMARLLGIPVALMIAITFIYSAALGGLAGVLVGPVLFVSIG
                     MGSIIALKAFAATIIGGFGDVKGAIVGGIFIGVIESFAAYYISVPYKDAYAFMMLFLV
                     LVFRPQGFFGEKVSEKA"
     gene            11659..13473
                     /locus_tag="VE030_00985"
     CDS             11659..13473
                     /locus_tag="VE030_00985"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HZF22020.1"
                     /translation="MKSLGGLQTTASRPRRFSPWLFLLIPAAAAVPLIPYATEVSHFA
                     VSMFMQAVTYAIAVLGMVVVLGYTGQINLAQAAFFGFGAYGVALGTVSYGLPFWVSLG
                     MGIGVAAIAGGILGLTTLRLGGHYLAMITISFQQIFDLVAVNWIAFTHGPDGIAGIPR
                     PSLFGHELADDRAYLLLCTLILYGLIALVWWLPQTRLGRAMRGVRENELAAEVTGVHT
                     LRVKVTAFTLCAALGGTGGGLYAGGFAYISPDNFNFTRAVEFLSMLLLGGAQSPFGGV
                     LGTTLLILLPEWLKEMPPSLQFIKDVYLAIYGLAVILIMVFMPEGLWGLLRNGWMKLR
                     SPIPVDTSNIPQLDLDIAVTETTPLLKLEGLQKFFGGLRAVDGIDLEIGRGTVHALIG
                     PNGSGKTTMLNVVNGIYKPTGGRIIIDGQDVSQKTPHERAGHGLGRTFQNIRLFAAMS
                     ALENVIVGAQRKNNPIEPGRAALRTRAMSALQFVGLAYKADTIVRSLPYGHQRLVEIA
                     RALAGHPKLLLLDEPAAGLNQTEKMELVELLRRLRSGHGLTIFLIDHDMGLVEKVSDR
                     ITVLNFGKKIAEGAPQEVLRHPEVIAAYLGVGPANGAA"
     gene            13466..14182
                     /locus_tag="VE030_00990"
     CDS             13466..14182
                     /locus_tag="VE030_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZF22021.1"
                     /translation="MLELKNVASYYGSVQALKGVSLRVEQGEVVTLLGANGAGKSTTL
                     RTISGLLRPASGEIWFEGQPIHDMAAEKIVRLGISHVPEGRRIFPGLTVRENIMLGTS
                     NRGRLPKKQMDQEVAQMFEVFPVIKPFENALGWTLSGGQQQMLAVARGLMARPKLLLL
                     DEPSLGLAPVIVQQLFGTIRQIHALGTTILLVEQNAHMALSVANRGYVLETGEMKIEG
                     SPKELLGNEEVRAAYLGGHG"
     gene            complement(14298..15293)
                     /gene="egtD"
                     /locus_tag="VE030_00995"
     CDS             complement(14298..15293)
                     /gene="egtD"
                     /locus_tag="VE030_00995"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HZF22022.1"
                     /translation="MKTLREPSAARRPANVTVISLGSGGEIDSGEVIAGLAGEPKRLS
                     PKLFYDARGSELFNAICGTDAYYPTRTERSILAGHAEAIARVVGPDCAIFEFGPGDMS
                     KVRLLLPRLLPAAYVGIDIAKVQLIAAAEALAYEYPWLEVFAVCADFGGELPVQLPPP
                     PGRRRVVFFPGSTIGNFEPDEARAFLGLLARMVGADGGVIVGVDFQKQTALLDRAYND
                     PEGLTRAFNLNVLTRLNRELGADFDERRFRHHAFYNESLGRVEMHLVSMVSQVVHLGG
                     RQIRFDAGETIHTENSYKYTPETFAALANEAGFGAHRLWTDARNYFGVFWLAASP"
     gene            15466..16734
                     /gene="egtB"
                     /locus_tag="VE030_01000"
     CDS             15466..16734
                     /gene="egtB"
                     /locus_tag="VE030_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HZF22023.1"
                     /translation="MNRPLHFHSDALSRNGLLEDLHRVRGLTVRLCDPLRAEDCVVQS
                     APETSPAKWHLAHTTWFFETFLLIPYLPKYRPFHPSFGFLFNSYYESVGEFHPRLQRG
                     LLSRPSLEEIKAYRDYVDQHMGMLLESPDTADAGEIRARARLGIHHEQQHQELLLTDI
                     KHLFACNPLKPAYREAIAPDAGTNPPLDWLRLPAGMYAFGHGADGFAFDNEMPRHRRY
                     LDEFSLASRPVTNAEYIEFIAAGGYRQPTLWLSDGWRKVNEARWTAPLYWEQQDSVWW
                     QMTLAGYRRVQPSEPVCHVSYYEADAFARWAGKRLPTEFEWEAAAGMQAPGGNLLESG
                     RFHPQPARDGASQFFGDVWEWTQSAYSPYPGYRPPAGALGEYNGKFMVSQLVLRGGSC
                     VTPATHIRASYRNFFYPQDRWQFMGIRLAA"
     gene            16757..17278
                     /locus_tag="VE030_01005"
     CDS             16757..17278
                     /locus_tag="VE030_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22024.1"
                     /translation="MMKFLLGLAVSAALAAAASAQTGLKEIQKDWPDFRYLEPKQVRE
                     LPALIKADLEKRGCKIPSFTKWDARHNVIQGQFLKPGQRDWAVLCRHQDKSSILIYPG
                     SATADVQMVRSEDHDPYRIIHTATAFVLHKRAIRDNPAEPPPQFDHDGIEDGPIQKAA
                     IVLYYRDGEWVEF"
     gene            17286..17873
                     /locus_tag="VE030_01010"
     CDS             17286..17873
                     /locus_tag="VE030_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HZF22025.1"
                     /translation="MRRVCVFCGSNAGARSEYAEAARTLAAVLVERKLGIVYGGGNVG
                     LMGVLADAALAHGGEVIGVIPQSLLDKEVGHRGVTELLVVETMHERKALMNDLADAFI
                     AMPGGFGTLDEFFEVLTWSQLGFHGKPCALLNVAGYYDRMLAMLDHAVTERLLRPAHR
                     ELVIADTDPLRLLHRLSAFSAAAKGKWGEAKGNFL"
     gene            17991..18470
                     /locus_tag="VE030_01015"
     CDS             17991..18470
                     /locus_tag="VE030_01015"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HZF22026.1"
                     /translation="MKKTLLSMTLTTLLVAAGTAVAIEQRPILTLEVAKKMAAACEAR
                     AKQEGWKMNIAILDSGGNMKHFSRMDDSFLKSIEIAQLKASTSAGLPFSTKQIGEIAA
                     KVPGIAFVPGIVTFEGGLPILTADGKHIGSIGVSGASAEQDGLCAQAGLDAVKNDLK"
     gene            complement(18520..19179)
                     /gene="larB"
                     /locus_tag="VE030_01020"
     CDS             complement(18520..19179)
                     /gene="larB"
                     /locus_tag="VE030_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008543072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HZF22027.1"
                     /translation="MTLHKDVMLDFARDARTGLDEAVMCASKSVLHLTTILDQVREKG
                     RCILLTRLLPEQFAELPAQHRRSIDYEPLSRTGFFGGSHQVKTETQIAVVAAGTSDVA
                     VGREAARTLRFYGEPAVEINDVGVAGLWRLLERVEEMRKMKVVIAVAGMDAALPTVIG
                     GLVSSVVIGVPTSVGYGVATGGETALRAMLASCSPGLLVTNIDNGFGAACAALRVLRA
                     A"
     gene            complement(19401..>19747)
                     /locus_tag="VE030_01025"
     CDS             complement(19401..>19747)
                     /locus_tag="VE030_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22028.1"
                     /translation="DLAEVAELPAAPCLSSRIETGIAIDPGMLKLVHAAERLVSQALT
                     PRTVRCRVRAQGVVVELDEQNLAALDSSRRQSLSAQVGRLFSDGGFDYPVSLAPYRSG
                     SAFLVRVARDLA"
BASE COUNT         3863 a         6177 c         6105 g         3602 t
ORIGIN      
        1 ccgcgttcgc gttcgccgcg gacgatgcgg aaatcaagaa aatccgggaa gaaattgaat
       61 ccatcaaaga cggctacgaa acgcgtatcc gccagctcga gcagcgcctt gagaaagccg
      121 agcaaagcac cgccacggca aagcccgccg cacctgcgca atccgcgccc gcttcaccga
      181 acgcattcaa ccctgcgatc tcgctgatcc tgggggggca gttcaacaac ctgcagcggg
      241 atccgggaac ctatcagatc ggcggcttca ttcccggcgg ggacgaaatc ggtcccggca
      301 atcgcagttt caatctcggc gagtccgaac tcaccgtgtc ggcgagcatc gatccctatt
      361 tctccggttt cttcgtcatg gcagtcaccc cggagaatgc agtctcggtg gaggaaggct
      421 atgtgcatag caccggcctc gttcccggcg tcactttgaa gtttggccgc ttcctgtccg
      481 gattcggtta tctcaacgaa atacacgccc acgcctggga cttcgtcgac gcgccgttgg
      541 tgcaccaggc atttttcggc gggcaactga aggaagaggg gctgcaggca cgctggctgg
      601 cgccgacccc ggtattcctg gaattcggac tggaaacggg acgcggcgcg aacttcccgg
      661 gctcggagcg caacaagaac ggcgtgaact ccggaatgat cttcgcgcat gtgggcgacg
      721 atgtcggcat cagcaacagc taccgcttcg gcgcgtccta ccgggtcgcg cacgcgcagg
      781 atcggccgta tgacgacttc gacgccagcg gaacgacatt agtcaccaac gccttcaccg
      841 gcgacagcaa gatgtgggga gtggatttgg tctggaagtg ggcgccgaac ggcaatacgg
      901 tggatcgcaa cttcaagttc caggcggaat accttcgccg caaggaagac ggcgatctcg
      961 tgttcgatac cgcgggcctc accccggcgg ccggtgcaag cggtgcctat cgctccagcc
     1021 agtcgggctg gtacatgcag ggcgcctacc agttcatggc gcgctggcgc gtagggttgc
     1081 gctacgaaga actggacagc ggcaacaccg atgtcggtct ggtcaacagc ggtacgctca
     1141 ctgcggccga cttccctcgc cttgcgcaga acgatcccaa gcggacgacc gcgatgatcg
     1201 acttctcgcc gtcggagttt tcgcgcttcc gcctgcagtt cgcgcgcgac gaggccaggt
     1261 tcaacgaagc cgataaccag gttttcctgc aatacatcat gagcctcggc acgcacggcg
     1321 cacacaaatt ctgaaccggc ccgcgatgat cgacgaaaca tcatgcgcat tgacgcgcaa
     1381 cagatacgag aggatacgga catgaacaga tggctgacat tggcgggcgc cgcagcgtta
     1441 tcgcttgcgt gctccccggt atttgccgcg gtgaatgttt tcgcctgcct gcccgagtgg
     1501 gcggcgctgg cgagcgaact cggcggcgac aaggtttcgg tttaccaggc ttcgaatgcg
     1561 cttcaggacc cgcaccgaat cgaggctcgc cccagcctgg tggcacgcat gcgcagcgcc
     1621 gatctggcga tctgcaccgg tgcggagttg gagatcggct ggctgccggt gctgttgcag
     1681 accgccggaa accagaaagt ccagccgggc aagccgggtt tcatcgccgc ggcggaattg
     1741 gtcgaccggc tggaaattcc aacgcgcctg gatcgcgcgg aaggcgatat ccatccggca
     1801 ggcaatccgc acatccaact cgatccgcac aatgtcgcca aggtggcggc cgtggtgacg
     1861 cagcgactgc agcagctcga tcccgcgaat gccgcctact acgacacgcg cggcaaggac
     1921 ttccaggcgc gctggcaaca agccatcggg cgctgggaaa aggaagcggc gcccctgaaa
     1981 ggtctgcgca tcgttcccta tcacaagaac acggtgtatc tgtcccattg gctggggatg
     2041 agtgaggtca tgtacataga gcccaaaccg ggcattcccc cgagcgccgg tcatctttcc
     2101 gagctggtgg tgcgtttaca ggccgagccc gccgacgcgg tgattcgcac ggcataccag
     2161 gatcccaagg cggcctcgtg gttgtctgaa aaaaccaaga tcccggtcgt ggaattgccc
     2221 tacaccgtgg gcggaacgcc cgcagccagg gatcttttcg gactgttcga cgacaccatt
     2281 gcgcgcctga acaaagtgaa aaagtaacat ggccgacctc gaattcagca ttctgcttcc
     2341 ggcgtttctc gccggattgc tggtgaccgc cacccatgtg ccgctgggaa tccaggtgct
     2401 gaatcggggc atcgttttca tcgatattgc aatcgcgcag attgctggtg taggcgtcat
     2461 agccgcccac tttttcggac tggaggacca agcctacgcg gtgcaggtcg cggcgctggg
     2521 cgccgcgctg ttgggcgcgt tgtttctgaa ttggaccgaa aagcgctggc caaccgtcca
     2581 ggaagcgatc atcggctcgg tattcgtgct ggcagccacg ctcgaaatcc tgttgctggc
     2641 cggcaatccg catgccggcg aatacctgaa ggacatgctg gtcggacaga ttctctgggt
     2701 cggccatcat caactggtgg tcgttgccgc actaactttc atcattctgg ccgcgtggtt
     2761 cggcatcggc atggcccggt tcggccgcgt gggtttctac ctgctgttcg gccttgcggt
     2821 gaccgcgtcg gtgcagatgg tcggcatcta tctggtgttc agcagcctga tcatcccggc
     2881 tctggcgtcg cgcaacgcca cgcggctgcg cctggccaag gcctacgccg tcggcgcgct
     2941 ggggtatttt ttcgggttga tcgcatcgat tgtgttcgat ctgcccaccg gcgcgatcat
     3001 cgtctgggcc atggcggtgg tgggaatcgt attttccgca ttcgaacgtg gccgtccgtc
     3061 cgcggccgca gcttgatagt ctccagtaca atccctatct tgcagtcatt gccggcccgc
     3121 cgccggagcg acgggccctc cgttgtccca ccgcgcgccg gccgccgcgc caatcgccat
     3181 gagttccgcc aacatagagg tctcggagat ccagccccag ctgtcccgcg cagagcagga
     3241 agccgcggtg cagtacgcca acggccatgc cgatgccgcg atcgaaatcc tgggcgaggc
     3301 gcttgaggcg gaaaaagttg cgcccttacc cgcgtggctg atgctcttcg accttttccg
     3361 gatacaggga cgatggaccg acttcgagaa tctgtcgaaa cgctacacgg ccctcttcgg
     3421 acgcgcggcg ccggactggc tcagcgacga catcctgccc gaaggcctgt cggcggaatt
     3481 gcggcccggc ggcgtggctt acgtggagat cgacgggccg ctcggcgcgg cttccgcgcc
     3541 ggatcttgcg cggattcgcg ttgcggcggc caggcatccc gtggtccaca tcgatctgac
     3601 caagattcag catgtccagc aagaaggttg cgcactgctg tcgcaggaac tgcagtttct
     3661 cgccggtaac ggcaacggcg tcctgttcag cggcgccgag cgcatcgaga agatgctgcg
     3721 gcaggcagtg gatacgatgt ccaagggggc cgcgttctgg cagcttctgc tcgatctgca
     3781 gcggctgcag ggaaatcaga agaaattcga aagcatcgcg ctggagtatg ccttgtacgt
     3841 agagaccgac ccaccggctt gggaaccggt gctgacgccg gttctgcctt gcgaaaccgt
     3901 cgaagaaaag cgcggagaac cgcgctaccg gcccgagctg atccatatca ccggtgagat
     3961 gaccgggctg aaggatccgc aactgcaggt actgcagcag tttgcctctg atcgccagta
     4021 cgtgaacatc aacgtggcca ggctgcaacg catcgacttc gtgtgcgccg gaaaccttgc
     4081 caacgtcatc gccgcactga agggcaaggg caagaccgtg cgcgtcctgc acgccaacag
     4141 tctcgttaca acgctgttgc gcctgttcaa agtggacgag aatgccgccc tggtcggcaa
     4201 gtaaagcggc cgttcgcgca gccaccttca taccttccgg cctacatatt tatgagtgac
     4261 agcattcacg gtacaacgat cgtgtcggtg cgccgtggaa gtcgcgtcgc cctcggcggc
     4321 gacggacagg tgaccttcgg caatgtcgtt ctcaaggcca gcgcgcgcaa ggtccggcgc
     4381 cttttccacg agcgcatcct cgccggtttc gccggcggca cggcggacgc gttcactctg
     4441 ttcgagcgct tcgaggcaaa gctggaaaag catcaaggca acctgctccg ttccgccgtg
     4501 gagctcgcaa aggactggcg caccgatcgc atgctgcgcc ggctcgaagc gatgctggcg
     4561 gtggcggacc acgaaacctc gctgatcgtc accggtaacg gcgacgttgt cgaaccggaa
     4621 ctggggctgg tggcaatcgg cagcggcggc ccctacgccc aggcggccgc ccgagcgttg
     4681 ctcgaaaata ccgaactgcc gccgcaggac atcgtcaaga gatcgctgac catcgccggc
     4741 gatatctgca tctacaccaa ccagaatcat gtaatcgagg tactcgatta gggacgaggg
     4801 gcgagggacg tggggtgagg gagcagtgca atgagaccga attaatagtc cctaatcccc
     4861 agccccaaat ccctcgtccc tcgcccctcg tccctcaccc ctaatgtcct caatgacccc
     4921 ccaagagatc gtccacgaac tggataaaca tatcatcggc caggacgccg ccaagcgcgc
     4981 cgtcgcgatc gcgctgcgca accgctggcg gcgccaacaa gtaaccgagc cgttgcgcca
     5041 ggagatcacg cccaagaaca tcctgatgat cgggcccacc ggggtgggca agaccgagat
     5101 cgcgcggcgg ctggccaagc tcgccgacgc gcccttcatc aaagttgagg ccaccaagtt
     5161 caccgaggtc ggatacgtcg gcaaggatgt ggattcgatc atccgcgacc tggtggagac
     5221 cgccatcaag cagacgcgcg agcaggagac aggcaaggtc cgccaccgtg cggaggacca
     5281 ggccgaggaa cgcatcctcg acgtactgct gccgccggcg cgggagatcg gcttccaggc
     5341 cgaacaggaa gaccgcgaca gcgcgacgag gcaaaaattc cgcaagaagc tgcgtgaagg
     5401 cgagctcgac gacaaggaga tcgagatcga agtcgcggcg atgcacaccc agatggaaat
     5461 cctggcgcct cccggaatgg aagacctgac ttcgcagatt cagagcatgt tccagaacct
     5521 gggcggaacg cggcgcaaga cccgcaagct caagatccgc gaggcgatgc aattgctgat
     5581 cgaggaagag gcggccaagc tggtcaacga cgaggagctc aagctcaagg ctctcaccaa
     5641 cgtggagcag aacggtatcg tctttctcga cgagatcgac aagatcacca gccgccagga
     5701 gacctcgggc gcagaggtgt cgcgccacgg cgtgcagcgc gacctgctgc cgctggtgga
     5761 gggcaccacg gtttcgacca agtacggcat gatcaagact gaccacatcc tgttcatcgg
     5821 cagcggcgcc ttccatctgt cccggccgtc cgatctgatc ccggagctgc agggccgctt
     5881 tccgatccgc gtcgagctcg gcagcctttc ggtggaagac ttcgagaaga tcctgaccgc
     5941 gaccgatgcc tgcctcacgc aacagtacca ggcattgctc gaaaccgaag gcgtccagct
     6001 cgagttccgg caggagggca tccgccggct ggccgagatc gcctggcagg tgaacgaaag
     6061 gaccgaaaac atcggtgcgc gccgcctcta caccgtgatg gagaaattgc tcgaggacgt
     6121 atcgttcgaa gccgaccgcc accaggggca gaagatcgtc atcgatgccg cccgcgtcga
     6181 cactcggctg aaggaattgg cgcagagcga agatctggcg cgatacgtgc tctaagcacg
     6241 tccgggcggc tcgccggcca gcaattgcgg cgccggcgca ccgatccggt gccggcccgg
     6301 cgtttcccca aagcgcagct tttcctcata atcccgcaga ctgactgctg acccggttca
     6361 accatgccac aaggcacgac gttcaccctc gaagtcaacg ccaagctgcc gcgccgtctc
     6421 gcccggcttg aggagctcgc caacaacctc tgttacagct gggaccggca ggcgcgactg
     6481 ctgttctcgc gcctcgacac cctgctctgg gatgaagtcg gtcacaaccc gaaggctttc
     6541 ctgaaacggg tcgacgaaaa gaagctggtg gacgctgccg acgacccggt gttcctgggc
     6601 aattacaacc gggtgctctc cgagttcgat acctaccacg gcgaaccgat gcgcagggac
     6661 ggctccgagt ggctgagaca gagcgatctg gtcgcgtact tctgcgccga attcggattc
     6721 cacgagagtc tgccgatcta ctccggagga ctgggcattc tcgccggcga tcattgcaag
     6781 tcggcaagcg acatgcgtct acccttcata ggcgtgggcc tgctctatcg ccagggctat
     6841 tttcatcaga caatcgacac cgaaggcaac cagaacgccc tttactcgga ttccgatttc
     6901 gaagacctgc cggtgtcgcc ggtgttgcgc gatgacggct ccgaggtcag ggtcaaggtc
     6961 gatctcccgg ggcgcgccgt gcaggtgaag gtctggcagg taaaagtggg gcatgtggcg
     7021 ctgtacctgc tcgataccga cctcgtcgaa aactcccacg acgatcgcga catcacgcac
     7081 cgcctctacg gtggcgaccg gaccacgcgc atcgagcagg aaatcgttct tggcatcggc
     7141 ggcgtacgcg cgcttgtgga actggggctg aaacccaccg cctggcacat caacgagggc
     7201 cacccggcat tcctggtgct cgagcgcgtg cgcatgctgg tcgcccaggg actgactttc
     7261 gacgcggccc tcgaagcggt cgccgccaac accgtgttca cgacccacac gccggtacct
     7321 gcgggccatg accacttcag cgcggacatg atgcgcgact atttctcctg gctgcgcaag
     7381 gaactgggca tgagcgagga agatctgctc gcgctcggcc gggtggacgg caaccaggag
     7441 ttcaacatga ccgcgctggc gatccgcgga tcgcggttcc agaacggcgt cagccgtatt
     7501 cacggcagcg tgtccgcgcg catctgcgcg ccgctgtggc cggaagtgga acccgaggaa
     7561 aatccgcttt cgtacgtcac caacggcgtg catgtgccca ctttcctggc gccggagtgg
     7621 tccgaccagt tcgacaagtt tctcggcttc gactggtcgc accatgccaa caaccgggcg
     7681 ttctggcggc gcatcgacga catgccggac catctgttct ggagcgtgcg ccagtccctg
     7741 aaagcgcaga tgctgcacct ggtgcgccac cgggtgtcgc gccagcactt ccgcaaccgc
     7801 ggttccgaag cccacctcga ccgcctgctg cgctacgccg atccggtcaa tccgaacgtg
     7861 cttaccatcg gcttcgcacg ccgctttgcc acgtataagc gggcggcgct gctgttcgag
     7921 gatctcgact ggctgcgccg gatcctcggc gacgaaaagc gcccggtcct tttcatcttc
     7981 gccggcaagg cgcatccggc cgacgtgccc ggccaggaca tcatccgccg gctcacgcag
     8041 atggcgcacc tgccggactt cgagggcaaa atcctgctga tcgaggatta cgacctgcga
     8101 ctggcgcggc gcctggtttc cggggtggac gtgtggctga acaacccggt gtatccgctg
     8161 gaggcaagcg gcacttccgg catgaaggcg ggaatgaacg gcgtgctcaa cctctcggtg
     8221 ctcgacggct ggtgggacga gggttacgac ggcaagaatg gctgggcgat caaaccggtt
     8281 tcggaaagcc tggacgaggc gcgccgcaat ttcgaggaaa gccggactct ctacgagttg
     8341 ctgcaagacc aggtgattcc gctgtactac cggcgcggtg acatgggcta ttcggccgag
     8401 tggctgcaca tggcgaagcg ctccatcgcc acgctgctgc cgcgcttttc gtccacgcgc
     8461 atggtaagcg agtatctggc gaaattctat cttcccgcaa cgcgccaggg ccggcgctat
     8521 gctgagtcga acttcgaggc agcacgccgg cttgcagagt ggaagaagca tgtgcgcagt
     8581 tcctggcgca aggtcggcat acgccgcctg gacatgccga agaaacgcat ccccttcggc
     8641 gacaacgtgc gtttcgaagt cggggtcgat ctcgacggtc tgaagccgga ggatctgagg
     8701 gtcgaactcc tggttgccaa tcagatcagc cgcgacaacg gtacgcaggc gacctgctac
     8761 cagttccagt cggaaggggt gcgcaccgaa aagggcgaac atcgcttcgt gctggaactg
     8821 agccccgagc actgcggcaa gctcggatac cgcatccgca tgtatccgca tcacgagctg
     8881 ctgacccatc cgttcgagat gggcatgatg cgctggctat agtgtcctgt tccgctaatt
     8941 cgcggaacac actcagagag gcaacgccaa tgccggtacg gatcgatcgc ctcgatcact
     9001 tggttctgac agtcaaggac atcgacgcga cctgcgcgtt ctactcgcgt gtgctcggca
     9061 tggatgcacg taccttcggc gatggccgcc ggtcgctcca ttttggcgcg gcaaagatca
     9121 acctgcacca gcaaggcatg gaattcgaac ccaaggccgc caagcccggc cccggttctg
     9181 ccgatgtttg cctgatcgcg gctacgccgc tcgcggaagt gatccggcac ttgcatgaat
     9241 gcggagtcga gatcatcgaa ggcccagtgc aaagaaccgg tgcgatcggg ccgatactct
     9301 cggtctactt ccgcgaccct gacggcaacc tgattgagat ttccaactac ggttagtccg
     9361 atgcgacagg cgcattgctt ctgatggaac agcacgcatt cttcccggca accgagttac
     9421 actgcaagct acgggtctca gcagtgcgca ttccggtcat gtcaaccgcc gccttcgcgt
     9481 cacgtttgtg ttatgcgccc gggattttcg ggcgaacgtg aacggaatcg acgggtactg
     9541 cgacgcagcg cccggtcaaa ccagaacaac attcgggaga aaatcatgcg cgtatcccta
     9601 ttcggcgccc tggcaacggc cgtcaccgcg tcgctcatcg gactttcagc aattgcggct
     9661 gaccccatcc gcatcggtct cgtcagcgag atcaccggcc cgaacgcgga agctggcggc
     9721 tacaccatca acggcgccaa actcgccgtc gaggaaatca acaagaaggg cggtatcctc
     9781 ggtcagcagg tcgagctggt ggtcgaagac aaccaatcca ccaatcccgg cacagtgctg
     9841 gcgatttcga aactcggcgg acgcaaggat attccggcga tcatcggacc gatccgcagc
     9901 acccagaccc aggcggcctc gcccaccatc gccaagctcg gcatccccac catgatcggc
     9961 ggcaccgatc ccagcctgac cagggtcaac aaccgctggc tcttccgctg ccgtcctaac
    10021 gatctgtact cctcccgcgt gatcgcggat ttcggcgtta attcgctgaa gttgaagaag
    10081 tgggcggtgg tgcactcgac cgatgccttc ggttccggcg gctcgaaagc gctgaccgat
    10141 gcgctcatgg cctatagcgt ggagccggtg ctggtccagg gctataccaa caattcgcag
    10201 gacttcactc cggtggtgct ggcaatcaag aaatccggcg cggacatcat cggcacctac
    10261 atgaccaatt cgcaggatct cggcatattc gccaagcagc tgcgccagct cggcgtcaat
    10321 gccacgtggg tcggctccgc ctcgatcgtc accgacaccg cgatgaagct cgccggagag
    10381 gcgttgtacg gtacctatgg cgtggccgat ttcaaccccg acgccaacga tgaggcaaaa
    10441 gcattctcga agatgtatcg tgccaagtac aagctggagc ccgacgtcta ttcgagctgg
    10501 gccttcgatg cgatgaacgt gatcgccacc gcgttgaacg gggccaagga cacgaagccg
    10561 gaaaacatcc gcaacggcat cgtcggcctg aaaaactgga agggcgtcga gggcatctac
    10621 aatttcgacg aggccggcga cggcctgcgc ggttacaaca tcgtcaagaa cgacaacggc
    10681 aaggtggcgt tcgtgaagca catcaacttc gaaaaataag cgcgacgatc gcgcaaccgc
    10741 gggatgcggg tgaacccgcg gcacagggga ggaaagcgcc gtggaaacgc ttgccaataa
    10801 tttccaggta ttgttccagg gcttagcgct aggcagtatc tatgccctgg tcgcgctggg
    10861 cttcgtgctc atcatccgcg ccaccaatgt agtcaacttc gcacaaggcg atttcgccat
    10921 gctcggcgcc tacatgatgg tcgcgtgtct tgtgggcgca ggattgccgt actggctgtc
    10981 gttcgtgctg gcgctgatcg gcatgggggt gttcggcatc ctcttcaact acggcgtgta
    11041 ctacccgctg cgacaccggg gattcctgcc ggtaatcatt tccacgctgg gcgcatccat
    11101 ctttctgcag aacaccgtgc tggcgatttt cggtgcgcag ccgaagcggc tcgacaacgt
    11161 gatccagggc ggcgtccaga tcggctcggt gttcctcgac agccagtacc tggtcatcct
    11221 ggcggtgacc atggtgatgg tggccttcca gtacttcctc ttcgagcaca cgctggtggg
    11281 caagaagctg caggccacgt cccaggacaa ggatatggcg cggctgctcg gaataccggt
    11341 cgcgctgatg attgcgatca ccttcattta cagtgccgcg ctcggtgggc tcgccggggt
    11401 actggtaggc ccggtgctgt tcgtttcgat cggcatgggt tcgatcatcg cgctcaaggc
    11461 ttttgccgca accatcatcg gcggcttcgg cgacgtcaag ggtgcgatcg tcggcggcat
    11521 tttcatcggc gtgatcgaga gcttcgcggc ctactacatc tcggttccct acaaggacgc
    11581 ctacgccttc atgatgctgt tcctggtgct ggtgttccgc ccgcagggct tcttcggcga
    11641 gaaagtgtcg gagaaggcat gaagtcgctt ggcggtttac aaacgactgc gtcgcgtccg
    11701 cgccgttttt cgccatggct gttcctgctg attcccgccg ctgccgcggt gccactcatt
    11761 ccgtacgcca ccgaggtcag ccatttcgcc gtctccatgt tcatgcaggc ggtgacctat
    11821 gcgatcgccg tgctcggcat ggtcgtggta ctcggctaca ccggacagat caacctggcg
    11881 caggcggcgt tcttcggatt cggtgcctac ggcgtcgcgc tcggcaccgt cagttacggc
    11941 ctgccgttct gggtttcgct cggcatgggc atcggcgtcg cagcgatcgc cggcggcatt
    12001 ctcgggctga ccacactgcg cctgggcggc cactatctgg caatgatcac catcagcttc
    12061 cagcagatct tcgatctggt agcggtgaac tggatcgcgt tcacgcacgg gccggacggc
    12121 atagccggca tcccgcgccc ctcgctgttc ggccatgaac tagccgacga ccgcgcctac
    12181 ctgctgctgt gcacgctgat cctgtatgga ctgatcgccc tggtctggtg gctgccgcaa
    12241 acgcggttgg gccgggccat gcgcggcgtg cgcgagaacg aactggcggc ggaagttacg
    12301 ggcgtgcata cgctgcgggt gaaggtcacg gcattcacct tgtgcgcggc gctcggcggt
    12361 accggcggcg ggctgtacgc cggcggcttt gcctacatca gtccggacaa cttcaatttc
    12421 acccgggccg tggaattcct gtccatgttg ctgctcggcg gcgcgcaatc accgttcggg
    12481 ggtgtgcttg gcaccacgct gctgatcctg ttgccggagt ggctcaagga aatgccgccg
    12541 tcgctgcagt tcatcaagga tgtctatctg gcgatctacg gcctggcggt aatcctgatc
    12601 atggtgttca tgccggaagg cctgtggggc ctgctgcgca acggatggat gaagttgcgt
    12661 tcgccaatcc cggtggatac ctccaacatt ccgcagctcg acctcgatat cgcggtaacg
    12721 gaaaccaccc cgctgctcaa gctggaaggc ctgcagaaat tcttcggggg cctgcgcgcg
    12781 gtggacggca tcgacctcga aatcggccgc ggcaccgtgc acgcactgat cggtccgaac
    12841 ggttccggca agaccaccat gctcaacgtc gtcaacggca tctacaaacc gaccggcggc
    12901 cgcatcatca tcgacggcca ggacgtcagc cagaaaacgc cgcatgaacg cgcaggacat
    12961 ggcctcggcc gcaccttcca gaacatccgc ctgttcgcag ccatgtcggc actcgagaac
    13021 gtgatcgtcg gcgcgcagcg caagaacaat ccgatcgaac cgggccgtgc tgcgctgcgg
    13081 acacgggcca tgtcggcgct gcagttcgtg ggcctggcct ataaggccga cacgatcgtc
    13141 aggagccttc cctatgggca ccagcgcctg gtcgagatcg cgcgggcgct cgccgggcat
    13201 ccgaaactgc tgctgcttga cgaacctgcc gccggcctga atcagaccga gaaaatggaa
    13261 ctggtggaac tgctcaggag gctgcgcagc ggtcacggcc tgaccatctt cctgatcgac
    13321 cacgacatgg ggctggtgga gaaggtgtcc gacaggatca cggtactgaa tttcggcaag
    13381 aagatcgcag aaggcgcacc acaggaagtg ctgcgccatc cggaagtgat tgccgcctat
    13441 ctcggtgtgg ggcctgccaa tggcgctgct tgagctgaag aacgtcgcca gttactacgg
    13501 cagcgtgcag gcgctgaaag gcgtgagcct gagagtcgaa cagggtgaag tcgtcacgct
    13561 gctgggcgcg aacggcgcgg gaaagagcac cactttgcgc actatctccg gcttgctgcg
    13621 cccggcgagc ggcgagatct ggttcgaggg acagccgatc cacgacatgg cggcggagaa
    13681 aatcgtgcgg ctcggcatct cgcacgtacc ggaagggcgg cgaattttcc ccggccttac
    13741 ggttcgcgag aacatcatgc tcggcacgtc caatcgcgga cgtcttccca agaagcagat
    13801 ggatcaggaa gtcgcgcaga tgttcgaggt tttcccggtg atcaagccct tcgagaacgc
    13861 actcggctgg accctctccg gcggccagca gcagatgctg gcagtggcgc gcgggctgat
    13921 ggccaggccg aaacttctcc tgctcgatga accgtcactg ggtctggcac cggtgatcgt
    13981 gcagcaactg ttcggcacca tccgccaaat ccacgcgctc ggtacgacca tacttctggt
    14041 ggaacagaac gcccacatgg cgctgtcggt tgccaatcgc ggctacgtgc tcgagaccgg
    14101 cgagatgaag atcgaaggtt cgccgaaaga actactcggc aatgaggaag tacgcgcggc
    14161 ctacctcggc ggccacggct agccgtcgaa tctgcgaccg cctatcgcca aggagcaatg
    14221 ctcggtagca acgctgagaa caagaaaacc gcacttctca cccgtggtga gatttttgaa
    14281 tcagggtttc cttatagtca cggcgacgca gccagccaga acaccccgaa atagttgcgt
    14341 gcatcggtcc acagccggtg cgcaccaaat cccgcctcgt tcgccagtgc ggcaaaggtt
    14401 tccggcgtgt acttgtagga attctcggtg tggatggttt cgccggcatc gaagcggatc
    14461 tgtcttccgc cgaggtgcac gacctgcgag accatgctga ccagatgcat ttccacgcgc
    14521 ccgagggatt cgttgtagaa ggcgtgatgg cggaaccggc gttcgtcgaa atccgccccc
    14581 agttcgcgat tgagccgggt aaggacattg agattgaacg cgcgggtcag tccttcggga
    14641 tcgttgtagg cgcgatcgag cagcgccgtc tgtttctgaa aatccacacc gacgatgacg
    14701 ccgccgtccg ctcccaccat gcgagcgagc aatccgagga atgcgcgcgc ctcatcgggt
    14761 tcgaagttgc cgatggtcga gccggggaaa aacacgacac ggcggcgccc gggcggcggc
    14821 ggcagttgta ccggcagttc gccgccgaag tcggcgcaga cggcaaatac ttcgagccag
    14881 gggtactcgt acgcgagcgc ttctgcggca gcaatgagtt gcactttggc gatgtcgatg
    14941 ccgacatagg ccgcgggcaa gagtcgcggc agcagcaggc gcaccttcga catgtccccg
    15001 gggccgaatt cgaaaatcgc gcagtccggt ccgaccaccc gcgcaattgc ctcggcgtgg
    15061 ccagcgagga tgctgcgctc ggtgcgcgtt gggtagtacg cgtcggttcc gcaaatagcg
    15121 ttgaaaagct cgctgccgcg ggcgtcgtaa aaaagcttgg gcgacaggcg tttgggctcg
    15181 ccggcgagcc cggcaatcac ttccccggaa tcgatttcac cgccgctgcc gagcgaaatc
    15241 accgtaacat tggcgggacg ccgcgcggcg gaaggttcgc gtagggtttt cattgaagtc
    15301 cttttgggcg ctgcgcctcc aaacctacgc caatctcgtt gcggagaaaa gtcggggttg
    15361 taagcacctg aaatctggag aaatgtcgcc ggctgacgac gatacggcgt ttgtcggccc
    15421 cgcacaatcg gaattacact tgcagacatc gcccggggaa gcacgatgaa tcgtccgctc
    15481 cattttcact ccgatgcctt gagccgcaac ggactgctcg aagacttgca tcgcgtccgc
    15541 ggccttacgg tccgactgtg cgacccgctg cgggcggagg attgcgtggt gcaaagtgcg
    15601 ccggaaacca gccccgcaaa atggcatctg gcgcacacca cgtggttctt cgagaccttt
    15661 ctgctcatcc cctacctgcc gaagtaccgg ccgtttcatc caagcttcgg cttcctcttc
    15721 aattcctatt acgaaagcgt gggcgaattc catccgcggc ttcagcgcgg cctgctgtcc
    15781 cggccctcgc tggaggaaat caaggcatac cgggactacg tggatcagca tatgggcatg
    15841 ttgctcgagt cgcccgacac tgccgatgcg ggagagatcc gggcccgcgc cagactcggg
    15901 attcatcacg aacaacagca ccaggaactg ctgctcaccg acatcaagca tctgttcgcc
    15961 tgcaatccgc tgaagcctgc gtatcgcgag gcaatagcgc cggacgctgg caccaacccg
    16021 ccactcgact ggctgcgcct cccagccggc atgtacgcct tcgggcacgg cgcagacgga
    16081 ttcgccttcg acaacgagat gccgcgccac cgtcgctacc ttgacgaatt ctcgctggcg
    16141 tcgcggccgg tcaccaacgc cgagtacatc gagttcatcg cagccggcgg ctacaggcaa
    16201 ccgacgcttt ggctttccga tggatggcgc aaggtcaacg aggcgcgctg gaccgcgccg
    16261 ctgtactggg agcaacagga cagcgtgtgg tggcagatga cgcttgccgg gtatcggcga
    16321 gtccagccca gcgaacccgt ctgccatgtg agctactacg aggccgatgc gtttgcacgc
    16381 tgggccggca agcgcctgcc cacggaattc gagtgggaag ccgccgcggg catgcaagcg
    16441 ccgggcggca atttgctgga aagcgggcgc ttccatccgc aaccggcccg cgacggcgcc
    16501 agccagttct tcggcgacgt gtgggaatgg acgcaaagcg cctacagccc gtatcccggc
    16561 taccggccgc cggccggtgc gctcggcgag tacaacggca agttcatggt cagccagctg
    16621 gtgttgcgcg gcggctcctg cgtcacaccg gctacgcata tccgcgccag ctaccgcaat
    16681 ttcttctacc cgcaggaccg ctggcagttc atggggattc gtttggccgc atgagccttt
    16741 cgcaggaggc cgcgcaatga tgaaattcct actcggattg gcagtctcgg ccgcgcttgc
    16801 cgctgcggca agcgcgcaga ccgggttgaa ggagatccag aaggattggc ccgatttccg
    16861 ctacctcgaa ccgaagcagg tgcgggaact gccggcgctc atcaaggccg acctggagaa
    16921 gcgcggctgc aagattccca gctttaccaa gtgggatgcc cggcacaacg tgatccaggg
    16981 ccagttcctc aaacccggtc agcgggactg ggcggtactg tgccggcacc aggacaagag
    17041 cagcatcctg atctacccgg gcagcgcgac cgcggacgtg cagatggttc gcagcgaaga
    17101 ccacgaccct taccgcatca tccacaccgc caccgcgttc gtgttgcaca agcgcgccat
    17161 ccgcgacaat ccggctgagc cgccgcccca gttcgatcac gacggcatcg aagacggtcc
    17221 gatccagaag gccgcgatcg ttctctacta tcgcgacggt gagtgggtcg agttctgaat
    17281 ccgggatgag gcgcgtctgc gttttctgcg gctctaacgc cggggcccgg agcgaatatg
    17341 cggaagcggc gcgcacgctg gccgccgttc tggtcgagcg caagctgggc atcgtttacg
    17401 gcggcggcaa tgtcgggctg atgggcgtgc tcgcggacgc ggcgctggca catggcggcg
    17461 aagtgatcgg cgtcattccg caatccctgc tcgacaagga ggttggccac cgtggagtga
    17521 ccgaactcct ggtggtggaa acgatgcacg agcgcaaggc gctgatgaac gacctcgcgg
    17581 acgcctttat cgcgatgccg ggcggattcg gcacgctcga cgagtttttc gaggttctca
    17641 cctggtcgca actcggtttc cacggcaagc cgtgcgcctt gctcaacgtc gccggatact
    17701 acgaccgcat gctcgccatg ctcgaccacg ccgtcacgga gcgcttgctg cgtccggcac
    17761 atcgcgaact tgtcattgcg gataccgatc cgttacggtt gttgcatcgc cttagcgctt
    17821 tttccgcggc tgcgaaagga aagtggggcg aggcgaaggg gaactttctg taatcgacgt
    17881 atcaaactcc ccggggtata ttgacacccc gtttttcggg ggacacggcg gatcaaatcc
    17941 gatccgcgtc tcgtatttcg gcggtattca aatcagagag gaaaataacc atgaagaaaa
    18001 ccttgctatc catgacttta acgacgctgc tggtggccgc gggcacggct gtcgcaatcg
    18061 agcagcgccc gatcctgacg ctggaagtcg ccaagaagat ggcggcggca tgcgaagcca
    18121 gggccaaaca ggaaggctgg aagatgaaca tcgccatcct ggattccggc gggaatatga
    18181 agcatttctc ccgcatggac gactcgttcc tgaagagcat cgaaatcgcc cagctcaagg
    18241 caagcacctc ggccggcttg ccgttctcca ccaagcagat cggcgagatt gccgccaagg
    18301 taccgggcat cgcattcgtg cccggcatcg taacgttcga gggcggcctg ccgattttga
    18361 ccgcggacgg caagcacatc ggttcgatcg gcgtgtccgg cgcgtccgcc gagcaggacg
    18421 gcctctgcgc ccaggccggg ctcgatgcgg tgaagaacga cctgaagtaa gttccggaga
    18481 aagagacggc ccggtcggct cgcggcggcc gggctttttt tacgcagcgc gcaggacgcg
    18541 cagtgcggcg caggcagcgc cgaaaccgtt gtcgatgttg gtgaccagca gtcccggaga
    18601 acagctcgcg agcatggcgc gcagagcggt ttcgccgccg gtggcgactc cgtagccgac
    18661 cgaggtcggc acgccgatca cgacactgga aaccagcccg ccgatcacgg tgggcaatgc
    18721 ggcgtccatt ccggcgaccg cgattacgac tttcatcttg cgcatctctt cgacgcgttc
    18781 cagcaggcgc cacaatcccg caacgccgac gtcgttgatt tcgactgcgg gttcaccgta
    18841 gaaacgcagc gtgcgcgccg cctctcttcc gaccgccacg tccgaagtac cggccgccac
    18901 gacagcgatt tgcgtttcgg tcttgacttg gtggctgcca ccgaaaaatc ctgtccgcga
    18961 cagcggttcg tagtcgatgc tcctgcgatg ctgcgccggc agctcggcga actgttcggg
    19021 cagcagccgt gtaagcagga tgcacctgcc cttttcccgg acctggtcga ggatcgtggt
    19081 cagatgcagc acggacttcg acgcacacat taccgcctcg tccagcccgg tgcgggcatc
    19141 gcgcgcgaaa tccagcatta cgtctttatg cagtgtcatg ttggctcaac ccctaaactc
    19201 tcgacgcaga ggacgcagag gagaagcaag agaggacaca gagaagatcg agacaagaaa
    19261 cgaacaggtg cgagcatttg gcgtgattgg caacctggcg gaacaattcg cacaatgtat
    19321 agcgtttctc gacgagtaag cttttctctt gtttttctct ctcgttcttc ctctgcgtcc
    19381 tctgcgtcaa cgctttggct ctaggcgagg tcgcgcgcca cgcgcacaag aaaggcactg
    19441 ccgctgcgat aaggcgcaag cgacaccgga tagtcgaaac ctccgtcgct gaacagccgg
    19501 cccacctgtg ccgacagcga ctgccgccgt gatgaatcga gcgccgcaag gttctgttca
    19561 tccagttcca ccactacacc ctgtgcccga acccggcaac gcaccgtacg cggcgtcagt
    19621 gcctgcgaca caagccgctc cgccgcgtgc accagcttga gcatgcctgg atcgatcgcg
    19681 attccggttt ctatccggct cgacaggcag ggtgccgccg gcagttcggc gacctccgcc
    19741 agatcga
//
LOCUS       DASQOP010000029         3920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1622965, whole genome shotgun sequence.
ACCESSION   DASQOP010000029 DASQOP010000000
VERSION     DASQOP010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1622965"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..1077
                     /locus_tag="VE030_01030"
     CDS             223..1077
                     /locus_tag="VE030_01030"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HZF22029.1"
                     /translation="MRRDRRRALFWSYVFLIIFVIFFLTPPIYMLITSLKTSAEISAA
                     TNPWWVFAPTLQNYIELLTSPVYLTFFRNSAVVSVLVVVISMLVSIPAAFSLSRMRFW
                     GSTTLATGVFLTYLIPDTLLFIPLFKIFAFLHDLTGIEFINHWWVLIIIYPTLTIPFC
                     TWIMIGYFSSIPKELDEAALMDGAGYMQTLIMIFIPVALPGIIAATIFAFTVSWAQFL
                     YPLAFTTSADQLVLPVGIVTTLIKGDVFNWGQIMTGALLGAAPPLVIYAFLMDYYIAG
                     LTAGATKG"
     gene            1125..2225
                     /gene="ugpC"
                     /locus_tag="VE030_01035"
     CDS             1125..2225
                     /gene="ugpC"
                     /locus_tag="VE030_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HZF22030.1"
                     /translation="MAEVTLRQVTKTYDDTPAVRGIDLQILDKEFVVLVGPSGCGKST
                     TLRMIAGLEDITGGEILVGGAVINDVPPKDRDIAMVFQNYALYPHMTVYENMSFGLRL
                     KKVAKDEIRVRVENAARILDISALLERKPKQLSGGQRQRVAMGRAIVRNPKVFLFDEP
                     LSNLDAKLRVQMRIEIKRVHQKVRTTTIYVTHDQVEAMTLADRVVVMNDGLIEQVGAP
                     NDLYHAPKTKFVAGFIGSPAMNFAPCKLEEMAGGLQVRLNEDIAFPVPQGRAGRYRPH
                     LHRSDLLFGLRPEHILERRSQPEPNLHAFDVTLEVTEPMGMETLVYFRVNGTEVCGRV
                     NPNAGAKAGAPMRLVADLNNMHLIDDASGKVL"
     gene            2303..3418
                     /locus_tag="VE030_01040"
     CDS             2303..3418
                     /locus_tag="VE030_01040"
                     /inference="COORDINATES: protein motif:HMM:NF007504.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HZF22031.1"
                     /translation="MKAEPLFVMATDLEGLVAAIFEKAGCSRIESERIARYLVRANLA
                     GHDSHGVVRVPRYIQLKHEKLVLADQEVDVLVDTPVLAVVDGKYGFGQTVAPQAVEVG
                     IGKCKHMGLAAVALRHAGHIGRVADWAEMAAVQGLVSIHFVNATAGVLVAPFGSVERR
                     FSTAPYCIGVPLPGRAPLLLDFATSTVAEGKVLVASQGGRPIPADALVGPDGQTSDDP
                     RLLYGEYRPGERRDIRKGLGALRAFGGHKGSGLALMCEILGGALTGNGCTAPERRFSN
                     GMFSLFVDPGRIDPDGLFPEEVVRYVAYVKAAKSSPPNTEILVPGEPEERTRRERLAQ
                     GVPLAEETWSSLLETARSVGLDEKRIGETQAARSVKA"
     gene            3445..>3920
                     /locus_tag="VE030_01045"
     CDS             3445..>3920
                     /locus_tag="VE030_01045"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZF22032.1"
                     /translation="MKQPLVLLPGLLCDAALWEPQLSELADVARFWVPDLTEYETMKE
                     MGESVLRDAPWKEFALAGLSMGGYVALEVMRQAPQRVTRLALLDTRARPERPDETGRR
                     RQLMKLAQSERGFTPVTSRMLPLLLHPSRVKDAQLTKTVRDMAERTGIEAYLRQQRA"
BASE COUNT          750 a         1179 c         1227 g          764 t
ORIGIN      
        1 ggtggaccag acgcacgtgt tcgccacctg ggcgtttcgg gtcggcatcg agagcaatga
       61 cattccactc ggcgcctgcg tctcgctgtt catgtttcca atcctcgcca ttgccgcgat
      121 cttcattctt cgcgacatca gtcggcgcgg aaacgaggcc tgaaaatgac ggcggccgcg
      181 cagcgctctg caactgttct ccgcgcggca cgaccggcca gcatgcggcg cgatcgccgc
      241 cgcgcgctgt tctggtccta cgtgttcctg atcatcttcg tgattttctt cctgaccccc
      301 ccgatttaca tgctgattac ttcgctcaag accagcgcgg agatttcggc ggcgacaaat
      361 ccgtggtggg ttttcgcgcc gacgctgcag aattacatcg agctattgac ctcgcccgtg
      421 tacttgacgt tcttccgaaa ctcggctgtc gtatcggtgc tggtcgttgt catcagcatg
      481 ctggtaagca tcccggctgc attctcactc tccaggatga ggttctgggg gtccaccacg
      541 ctcgcgacgg gagtattcct cacttatctg attcctgaca cgctgctgtt tattccgttg
      601 ttcaagatct ttgcgttcct gcacgacttg accggaatcg agttcatcaa ccactggtgg
      661 gtgctcatca tcatttatcc cacgctcacc atcccgtttt gcacctggat catgatcggc
      721 tatttcagca gcatcccgaa ggaactggac gaagccgcgc tcatggacgg cgccggatac
      781 atgcaaacac tcatcatgat cttcatccct gtcgccctgc ccgggatcat agccgcgacc
      841 atctttgctt ttaccgtctc ctgggcccag ttcctctatc cgctcgcttt cacgacctcg
      901 gccgatcaac tggtcctgcc ggtgggcatc gtcacgacgc tgatcaaggg cgacgtgttc
      961 aattggggcc agatcatgac cggcgcgcta ctaggcgccg cgccgccgct tgtcatctat
     1021 gccttcctta tggactacta catcgccggc cttaccgccg gcgccaccaa gggctaggcc
     1081 gtgcacgcaa ccttcctgcg actggatacc gtggagaccg accaatggcc gaggtaacgt
     1141 tgcgccaggt gaccaagacc tacgacgaca cccccgccgt gcgagggatc gatctgcaaa
     1201 tcctggacaa ggagtttgtc gttctggtcg ggccgtcggg ctgcggcaag agcacgacct
     1261 tgcgcatgat agcgggtctg gaggacatca ccggcggcga aatcctggtg ggcggcgcgg
     1321 tgataaatga cgtgcccccg aaggatcgcg acatcgccat ggtgtttcag aattacgccc
     1381 tgtacccgca catgacggtg tacgagaaca tgtcttttgg gctgcgcctc aaaaaggtcg
     1441 cgaaagacga gatccgggtc cgggtcgaga acgccgcccg gatcctcgac ataagcgcgc
     1501 ttctcgaacg caagccaaag caactctccg gcggacagcg ccagcgtgtg gcgatgggtc
     1561 gcgccatcgt gcgcaatccc aaagtgtttc tgttcgatga accgctttcc aacctcgatg
     1621 ccaagctgcg cgtgcagatg cgcatcgaga tcaagcgggt tcaccagaaa gtgcgcacca
     1681 ccaccatcta tgtcacccac gatcaggtcg aagccatgac gctagcggac cgggtggtag
     1741 tgatgaatga cggcctgata gagcaggtcg gcgcgccgaa cgatctctac cacgcgccga
     1801 agacgaagtt tgttgccggg ttcatcggct cgcctgcgat gaatttcgcg ccctgcaaac
     1861 tcgaggagat ggcgggcggg ctgcaggtcc ggctgaacga agacattgcg tttccggtgc
     1921 cgcaaggccg cgccgggcgt taccggccgc accttcatcg cagtgatcta ttattcggtc
     1981 ttcggccgga gcacattctg gaacgccgct cccaaccgga gccgaacctg catgcgttcg
     2041 acgttacact cgaggtgacg gagccgatgg ggatggaaac gctggtgtac ttccgggtga
     2101 acggcacaga ggtgtgcggg cgggtcaatc cgaacgcggg agcaaaagcc ggcgcgccca
     2161 tgaggctggt cgccgatctc aacaacatgc acctcatcga cgatgcgagc ggcaaggttc
     2221 tctgaagtca tggccccagc ctgcggcgag caggcgagcg ctgccgggta ggtgaggaat
     2281 tgctgacagg tctcgactag acatgaaggc agagcccttg tttgtgatgg cgacggacct
     2341 cgaaggattg gttgccgcaa tcttcgaaaa agcgggctgt tcgcggatcg aatcggaacg
     2401 catagcgcga tatctggtcc gcgccaacct ggccggccac gacagtcatg gggtggtgcg
     2461 ggtaccgcgc tatatccagt tgaagcacga aaagctggtg cttgccgatc aggaagttga
     2521 cgtgctggtc gacacaccgg tccttgcagt ggtcgatgga aagtatggat tcggacagac
     2581 cgtagcgccg caggcggtcg aagtcggcat cggaaaatgc aaacacatgg gtctcgcggc
     2641 cgtggccttg cgccatgccg gccacatcgg gcgagttgcg gactgggcgg agatggccgc
     2701 cgtacaaggg cttgtgtcca tccactttgt gaatgccacc gccggcgttc tggtggcgcc
     2761 gttcggcagc gtggaacggc gattttccac ggcgccctat tgcatcggag tccccctgcc
     2821 gggacgcgca ccgctgctgc ttgattttgc gacatccaca gttgcggaag gcaaggttct
     2881 ggtggcgagc cagggagggc ggccgattcc cgccgacgcg ctggtcggtc cggatggaca
     2941 aaccagcgat gatccgcggc tgctttatgg cgaataccgg ccgggcgaga ggcgggatat
     3001 ccgcaaggga ctgggtgcac tgcgcgcctt cggcggacac aagggatcgg gactggcgct
     3061 catgtgcgaa atcctcggtg gagcactcac cggcaacgga tgcactgcgc cggaacggcg
     3121 cttctccaac ggaatgtttt ccttgtttgt cgatccggga cgcatcgatc cggacggctt
     3181 gtttcccgaa gaagtggttc gttatgtcgc ctacgtgaag gctgctaaat cctcacctcc
     3241 gaatacggag atcctggtcc cgggggaacc cgaagagcgg acccggcgcg aacggttggc
     3301 gcaaggcgta ccgttggccg aagagacctg gagttcgctg ctcgagacgg cgcggtctgt
     3361 tggcctcgac gagaagcgca tcggggagac gcaagccgcg cgcagcgtga aggcctgacc
     3421 gccgcctctg gtcgtgaaga aaaaatgaaa cagccattgg tcctgctccc cggactgctc
     3481 tgcgacgcgg cgctgtggga accgcagttg tcggaactgg cggatgtcgc ccggttctgg
     3541 gtgccggatc tgaccgaata cgaaaccatg aaggagatgg gcgagtcggt gctgcgcgat
     3601 gcgccgtgga aggaattcgc gctcgccggt ctgtcgatgg gcggttacgt cgcgctggaa
     3661 gtcatgcgcc aggcgccgca gcgggtgacc cggctcgcgc tgctcgatac ccgtgcgcgg
     3721 ccggaaaggc ccgacgaaac cgggcggcgg aggcaattga tgaaactcgc acagagcgaa
     3781 cgcggcttca cgccggtaac cagccgcatg ctgccgctgc tgcttcaccc ctcgcgggtc
     3841 aaagatgcgc agctcaccaa gaccgtccga gacatggcgg aacgcaccgg aatcgaagct
     3901 tacctgcggc agcagcgggc
//
LOCUS       DASQOP010000030         8174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2629915, whole genome shotgun sequence.
ACCESSION   DASQOP010000030 DASQOP010000000
VERSION     DASQOP010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8174
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2629915"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VE030_01050"
     CDS             complement(<1..375)
                     /locus_tag="VE030_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007741571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZF22033.1"
                     /translation="MQIQQLTSPAGIVTLAALPPLSLYVHIPWCVRKCPYCDFNSHET
                     KGEIPEREYVASLIADLELALPQVWGRPIYTVFFGGGTPSLLSARALDELLSAIRARL
                     PLAPDAEITLEANPGTFEAQKFA"
     gene            494..1312
                     /gene="murI"
                     /locus_tag="VE030_01055"
     CDS             494..1312
                     /gene="murI"
                     /locus_tag="VE030_01055"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HZF22034.1"
                     /translation="MMQAGSVPRVSKNSPVGVFDSGVGGLTILRAVREALPHEDLVYV
                     ADSAHLPYGQKPAEFVRGRAIAITGFMVEQGVKAIVVACNTATAAALGTLRTRFALPF
                     IGVDPAVKPAATATRSGVIGVLATPTTLSSERFCALLDRFARDARVLQQPCTGLAEHI
                     ERGDIDDEATEALVRGFVEPLLAAGADTIVLGCTHYPFVAHIVQRIAGPQVTVIENGT
                     AVARELARQLAAHGIRNPSGAGRQTFWVSGAPADTERVLARLWAPEVCVRSLSF"
     gene            complement(1309..1626)
                     /locus_tag="VE030_01060"
     CDS             complement(1309..1626)
                     /locus_tag="VE030_01060"
                     /inference="COORDINATES: protein motif:HMM:NF025363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK family protein"
                     /protein_id="HZF22035.1"
                     /translation="MMSPRIAEIVSRREALILRAAAQRAQIAAAASRLGPSLWFANLA
                     VHVYRLVKSRPVWAALALGVLAVAGPRRALRWGYRGVTLFATVVPLAKVLADLLQRLR
                     AVP"
     gene            complement(1623..2030)
                     /locus_tag="VE030_01065"
     CDS             complement(1623..2030)
                     /locus_tag="VE030_01065"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HZF22036.1"
                     /translation="MDDRNTPDAPEDSPQVGLFDSLKTLLATLVGIAHTRVELISTEV
                     EEQFARLVSLLAWALVALFFAFTGVILSAIALIVVFWDSNRVPAAAGLAAAFVLLAVI
                     AVLGFIARAKARPRLFKASLEELAKDRSQLNSK"
     gene            complement(2040..2345)
                     /locus_tag="VE030_01070"
     CDS             complement(2040..2345)
                     /locus_tag="VE030_01070"
                     /inference="COORDINATES: protein motif:HMM:NF017742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HZF22037.1"
                     /translation="MNTEVTTDKLMEDLRLLVADAEELLKVTAGQAGEKVNNAREHAE
                     ESIRVAKARIADAGHVAAEHTREAVKAADDYIRENPWAAVGIAAGVGLLIGVLINRK"
     gene            complement(2462..3055)
                     /gene="rdgB"
                     /locus_tag="VE030_01075"
     CDS             complement(2462..3055)
                     /gene="rdgB"
                     /locus_tag="VE030_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HZF22038.1"
                     /translation="MLKRLVIASNNPGKLREIGQILAPLGIEIVAQSVLAVPEAEEPY
                     ASCIENALAKARHAARLTGLPALADDSGICVDALGGAPGVASAHFAGAPRSDARNNRK
                     LLEMLEAENNRRAHYYCVMVLMRHGDDPQPLVAEGEWRGEILREPRGSGGFGYDPLFL
                     DPALGKTGAELSPEQKNRVSHRGKALAALVDVLKQSA"
     gene            complement(3225..3947)
                     /gene="rph"
                     /locus_tag="VE030_01080"
     CDS             complement(3225..3947)
                     /gene="rph"
                     /locus_tag="VE030_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HZF22039.1"
                     /translation="MRPSGRSPVELRKLSITRGYTRHAEGSVLIECGDTKVICTASID
                     DKVPPFLKGKGQGWLTAEYGMLPRSTNTRTEREAARGKQSGRTQEIQRLIGRSLRAVT
                     RLDLLGEHTIQIDCDVIQADGGTRTASITGAFVALHDAATYLQARQTIGEWPIQDHVA
                     AVSVGVYQGVPVLDLDYPEDSACDTDMNVVMTGAGGLVEVQGTAEGVPFSRKEMDAML
                     DLAEKGIRQLIAAQRSALGLGN"
     gene            4108..4974
                     /locus_tag="VE030_01085"
     CDS             4108..4974
                     /locus_tag="VE030_01085"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HZF22040.1"
                     /translation="MIYSMTGYAVAAAELPFGALNLELRSVNHRYLDVQFRIPDDLRT
                     LEAGLREKTTGRITRGKVEVRITVNKTPASQSGLQIDDKLLAKLRQLNDDVMSAIPES
                     AALSVNDVLHWPGMLGNDALQLDDLQEACGRLLERALDDLTETRAREGEKLKNVLLER
                     IGAMETLVRNIAPRMPQLIAGYKDKLTARLREAMVNMDDDRLKQELVLFASKIDVDEE
                     LSRLNTHFGEIRRILDKGGAVGKRLDFLMQELNREANTLGSKSVDLEVTQTSMELKVL
                     IEQMREQIQNIE"
     gene            5149..5757
                     /gene="gmk"
                     /locus_tag="VE030_01090"
     CDS             5149..5757
                     /gene="gmk"
                     /locus_tag="VE030_01090"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HZF22041.1"
                     /translation="MIGNIFIVCAPSGAGKTSLVRELLARDPNVRLSVSHTTRAPRPG
                     EQGGRDYHFVTRPVFQAMLERAEFLESAEVHCNLYGTSQAWIEVHRGQGHDIVLEIDW
                     QGAQQVRKVIPEAIGIFILPPSLEVLRQRLMARRQDSAAVIERRLKAARGEIAHLEEF
                     DYVIINNNFDEAVGDLASIVRSARLRLLAQIERHIDLINSLK"
     gene            5765..5968
                     /gene="rpoZ"
                     /locus_tag="VE030_01095"
     CDS             5765..5968
                     /gene="rpoZ"
                     /locus_tag="VE030_01095"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HZF22042.1"
                     /translation="MARITVEDCLKHIPNRFELTLAATYRARQLATGASPMVDTNKDK
                     PTVIALREIAAQKVGVEILKSKA"
     gene            6072..>8174
                     /locus_tag="VE030_01100"
     CDS             6072..>8174
                     /locus_tag="VE030_01100"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HZF22043.1"
                     /translation="MSEAELLFKDAAGYLKPEDIAQLQNAYQFSESAHEGQFRKSGDP
                     YISHPIAVAQILAQWHLDGQALTAALLHDVMEDTAVTKTEISDKFGKHVADLVDGVSK
                     LDRIEFQTQEEAQAENFRKMLLAMARDVRVILIKLADRLHNMRTLQVMQPKKRARIAR
                     ETLEIYAPIANRLGLNQLYQELQELAFRHLYPNRFNVLSKALLRARGNRREVVDRILD
                     AIKQKLADSYIEATVSGREKNLYSIYKKMLEKNLSFAEVFDIYGFRVIVKDAAACYLA
                     LGALHQLYKPIPGKFKDYIAIPKGNGYQSLHTTLFGPFGTPIEVQIRTLEMHKIAEAG
                     VASHWLYKDDAQINELHMKTHQWLQSLLEMQSESGDSIEFLEHLKVDLFPDEVYVFTP
                     KGKIMALPHGATCVDFAYAVHTDIGNRCVAAKINHELVPLRSELKSGDRVEIITAAHA
                     NPNPAWLNYVVTGKARSHIRHFLKTMQYEESAQLGERLLSQALAALKSSPDRISRDQW
                     ERLLKENSAKTRQQVLADLGLGKQLAIVIARQLLSIAEPKGSKTEQLPGSLIIRGSEG
                     MALQFAKCCHPIPGDPIIGFLRKGHGLVIHTHDCPVARKTRGDPEKWLDVEWATDTKK
                     LFTVNIKMVVANERGVLAKIAAVIAETGSNIDNVSLEPGDSSAYTIIHFTLQVSNRLH
                     LAQIMRGLRRVPEVIRIWR"
BASE COUNT         1598 a         2606 c         2476 g         1494 t
ORIGIN      
        1 ggcgaatttc tgcgcctcga aggtccccgg gttggcttcc agcgtgatct cggcgtccgg
       61 cgcgagcggc agccttgccc ggatcgccga cagcagttca tccagcgcac gtgcggacag
      121 cagcgagggc gtgccgccgc cgaagaaaac cgtgtatatc ggcctgcccc agacttgcgg
      181 cagcgccagt tccagatcgg caatcagcga tgcaacgtat tcccgctccg gaatctcgcc
      241 cttcgtttcg tgggaattga agtcgcaata cgggcacttg cgcacgcacc acgggatatg
      301 gacgtacagc gacagcggcg gcagcgccgc cagcgtcacg atcccggccg ggctcgtcaa
      361 ctgctggatc tgcatgcgga caacgtcttg agatgtgtgc gagttctact ataacctgtc
      421 cgcaactcga gtcttgttct cagggcaacg cacagatgaa tcaactcccg gccggctccg
      481 gcgcagctgc gcgatgatgc aggccgggtc cgtgccgcgc gtctccaaga acagccccgt
      541 cggcgttttc gattcgggcg ttggcggtct caccatcctg cgcgcggttc gggaggccct
      601 gccgcacgag gatctggtct acgtcgccga ttcggcgcat cttccctatg ggcagaaacc
      661 ggcggagttc gttcgcggtc gtgcaatcgc gatcaccgga ttcatggtag aacaaggcgt
      721 gaaggcaatc gtggtggcat gcaataccgc aaccgccgcc gcgctcggta ccctgcgcac
      781 gcgctttgcg ctgcccttca tcggcgtgga cccggcggtc aaaccggcgg cgaccgcgac
      841 ccgcagtggc gtgatcggcg tgcttgccac gcccaccacg ctctccagcg agcgcttttg
      901 cgcgctgctg gatcgattcg cacgcgatgc ccgcgtgctg cagcagcctt gcacgggctt
      961 ggcggagcat atcgagcgcg gcgatatcga cgacgaagca accgaagcgc tggtgcgcgg
     1021 atttgtcgag cccttgcttg ccgccggtgc ggacaccatc gtgctcggct gcacgcacta
     1081 tccgttcgtt gcccacatcg tgcagcggat tgccggaccc caggtaaccg tcatcgaaaa
     1141 cggcactgct gtggcgcggg agctggcacg ccagcttgcc gcgcacggca ttcgcaatcc
     1201 ttcaggggcg gggcggcaga ctttctgggt cagcggagcg ccggcggaca ccgaacgagt
     1261 cctggcgcgt ctgtgggcgc cggaagtgtg cgtgcgatcc ctgtcttttt aggggacagc
     1321 gcgcagccgt tgcagcagat cggccagcac ctttgccaaa ggcacgacgg tcgcgaacag
     1381 cgtgacgcca cgataacccc agcgcaacgc acggcgcggt ccggccaccg ccagcacacc
     1441 cagcgccagc gcggcccata ccggccgcga tttgacgagg cggtagacgt gcaccgccag
     1501 attggcgaac caaagcgacg ggcctagcct agacgcggcg gcggcaatct gggcgcgttg
     1561 ggccgcagcg cgtaagatca atgcctcgcg ccggctcacg atctctgcaa ttctaggaga
     1621 catcatttgg aattgagctg gctgcgatcc ttggccagtt cttctaggct tgccttgaac
     1681 aatcgcggtc tggctttcgc tcttgcgatg aatcccagca ccgcgatcac cgccagcagt
     1741 acgaaagccg ccgccagtcc ggcggccgcc ggcacccggt tcgaatccca gaacacgacg
     1801 atcaaggcga ttgccgagag gataacgccg gtaaacgcga agaacagggc caccagcgcc
     1861 cacgccagca gcgacaccag tcgggcaaac tgctcctcga cctcggtgct gatcaactcg
     1921 acgcgggtgt gggcaatgcc gaccagcgtc gccagcagcg tcttgagcga gtcgaacagg
     1981 ccgacttgcg gactatcctc cggcgcatcc ggcgtattgc gatcatccat gcgcgtgcgt
     2041 cacttgcggt tgatgagaac gccgatcagg agccccacac ccgcagcaat gcccactgca
     2101 gcccacggat tctcgcgaat gtaatcgtcc gcggccttta cggcttcgcg tgtatgctcg
     2161 gcggccacgt gcccggcatc ggcgatacgc gccttggcca cgcggatgga ttcctctgca
     2221 tgctccctcg cgttgttcac cttttcgccc gcctggccgg cggtgacctt gagcagttcc
     2281 tcggcatccg ctaccagcag ccggaggtct tccatgagct tgtcggtcgt cacttcagta
     2341 ttcatttcgg actccataag ctttttcatc agcttgcggt gagacacaat gtatacgccc
     2401 gtcggggagc gtcatgcaaa tatagttccg gcgggtatca caaaaacgcg gcgagatgct
     2461 ttcaagcgct ctgcttcagc acgtccacca gtgccgccag cgccttgccg cgatggctga
     2521 cgcggttttt ttgttccggc gacagttctg cgccggtttt ccccagcgcc ggatcgagga
     2581 acagcggatc gtagccgaaa ccgccggagc cacggggttc gcgcaggatc tcgccgcgcc
     2641 actcgccctc ggcgaccagc ggttgcgggt cgtctccgtg acgcatcagc accatcacgc
     2701 agtagtaatg cgcccggcga ttgttctccg cctccagcat ttcgagcagc ttgcggttgt
     2761 tgcgcgcatc cgatcttggc gcgccggcaa agtgcgccga ggctacgccc ggcgcacccc
     2821 cgagcgcgtc cacgcagatg ccggaatcgt ccgccagtgc cggcagcccg gtaagccgcg
     2881 ctgcatggcg tgccttggca agtgcgtttt cgatgcaact ggcgtacggt tcctcggcct
     2941 ccggaaccgc gagcaccgat tgggcaacga tctcgatgcc gagaggtgcg agaatctggc
     3001 cgatttcgcg cagctttccc gggttgttgc ttgctatgac gagtcttttt agcatctaga
     3061 ccttgaaccg caaaggcgca aagaaaaaca cgcgaagaaa tgcgaaggaa tcgaatgcaa
     3121 tatgactacg ctgcgtggca aattgcctgc gaaaaactcc gaatcaacga aatatctctc
     3181 ttcggtttcc tttgcgttcc ctttgcgcct tcgcgccttt gcggttaatt gcctagtccg
     3241 agcgcggagc gctgcgcggc gatgagctgg cggatgcctt tttcagccag atccagcatg
     3301 gcgtccattt ccttgcgcga aaacggcacg ccttccgccg tgccctgcac ttcgaccaga
     3361 ccgccggcgc cggtcatgac cacgttcatg tcggtatcgc aggcggaatc ttccgggtaa
     3421 tcgaggtcca gcaccggcac accctgatac accccgaccg atacggccgc gacgtggtcc
     3481 tggatcggcc attctccgat cgtttgcctt gcctggagat acgtcgccgc atcgtggagc
     3541 gcgacgaacg caccggtgat gctcgccgtg cgcgtgccac cgtctgcctg gatcacgtcg
     3601 cagtcgatct gaatggtgtg ctcgccgagc agatcgagcc tggtgaccgc gcgcaggctg
     3661 cgtccgatca ggcgctggat ttcctgggtg cgcccggact gcttgccgcg tgccgcctcg
     3721 cgctcagtgc gcgtgttggt cgagcgtggc agcatgccgt attcggcggt cagccagccc
     3781 tggcctttcc ccttcaggaa cggcggcacc ttgtcgtcga tacttgcagt gcagatcact
     3841 ttggtgtcac cgcactcgat caacactgag ccttcggcgt ggcgggtata gccgcgggtg
     3901 atgctgagct ttctcaattc gacgggggag cgtccgctgg ggcgcatggt ggttctttca
     3961 tgatgtcggg ataaggggac cgcgggcgcc tgcggcgcgg atgctacagg ttttgtccgt
     4021 cggcaatatg ccgttgattt gtgtgctatc ttttcggctc gccaattctg gtcaagtcct
     4081 ttcaccttgt aaagcgccag ggccgccatg atctacagca tgaccggtta tgccgtcgct
     4141 gcggcagagt tgcctttcgg cgcactcaac ctcgaactgc gctcggtcaa tcatcgttat
     4201 ctcgacgttc agttccgcat ccccgacgat cttcgtactc tcgaagcggg gctgcgtgaa
     4261 aaaaccaccg ggcgcatcac gcgcgggaaa gtggaagtgc gcattacggt caataagaca
     4321 ccggcatcgc agtcgggcct gcagatcgac gacaaactgc tcgcgaagct caggcaactc
     4381 aacgacgacg tgatgtcggc aataccggaa agcgcggcgc tgtcggtcaa cgatgtgctg
     4441 cactggcccg gaatgctggg caacgacgcc ctgcaattgg acgatctgca ggaagcctgc
     4501 ggccggctgc tcgagcgagc gttggacgat ctcaccgaaa cccgcgcccg cgaaggcgaa
     4561 aaactgaaga atgtcctgct cgagcgcatc ggcgcgatgg aaacgctggt aagaaacatc
     4621 gcgccacgca tgccgcagct gattgccggc tacaaggaca aactcaccgc gcgcctcagg
     4681 gaggcgatgg tgaacatgga cgacgatcgg ctcaagcagg agctggtgct gtttgcctcg
     4741 aagatcgacg tcgacgagga attgtcgagg ctcaataccc acttcggcga gatccgccgc
     4801 atcctggaca aaggcggcgc ggtcggaaag cgactggatt tcctgatgca ggaactgaac
     4861 cgcgaagcca acacgctggg gtccaaatca gtggacctcg aagtcaccca gacatcgatg
     4921 gagctcaagg tgctgatcga gcagatgcgg gaacagattc agaatataga gtgaggcgta
     4981 aggagtgagg cgtgaggagt ggaaaactgg agaatggcga accgaagcca gagctagctg
     5041 gcagcggctc cggaggataa ctctcagtgg tccaggctca tttgcattgc gtaccgcatc
     5101 gcggtacgtg cttctcccct cacgcctcac gcctcacgcc tcacccagat gatcggaaac
     5161 attttcatcg tctgcgcccc ctcgggagcc ggcaagacgt ccctggtcag ggaactgctc
     5221 gcccgcgatc cgaacgtgcg cctgtcggtg tcccatacca cccgggcacc acggcccggc
     5281 gaacagggcg ggcgcgatta tcacttcgta accaggccgg tgtttcaggc catgctcgag
     5341 cgcgccgagt ttctggaaag cgccgaggta cactgcaatc tgtacggcac ttcgcaggcc
     5401 tggatcgagg tccaccgcgg ccagggccat gacatcgtgc tggagatcga ctggcagggt
     5461 gcgcaacagg tgcgcaaggt gattcccgaa gcgatcggca ttttcatcct gccgccctcg
     5521 ctggaggtac tgagacagcg cctgatggct cgccgccagg acagcgccgc cgtcatcgag
     5581 cgccgcctca aggcggcgcg cggcgagatc gcccatttgg aggaattcga ctatgttatt
     5641 attaacaaca acttcgatga agccgtgggg gatctggcaa gcatcgtacg atccgcacgg
     5701 ttgcgattgc tcgcgcagat cgagcgtcac attgacttga tcaacagttt gaagtgaagg
     5761 tgttatggcg cgtatcactg tagaagactg tctgaagcac attccgaatc gcttcgagct
     5821 gacgctggcc gcgacctatc gcgcgcgcca gcttgccacc ggcgcctctc cgatggtgga
     5881 caccaacaag gacaaaccca ccgtcatcgc attgcgcgag atcgcggccc agaaggtcgg
     5941 ggtcgagatc ctcaagtcca aagcctgacg gcgctgccgt ccctcacgat cgcgggaggt
     6001 tcagccaagc acacttccac cggtgaatgc ctgcggccca ccggtgcccg gctgaatccc
     6061 cagcgctgac catgtccgag gccgagcttc tcttcaagga tgcagccggc tatctcaagc
     6121 cggaggatat tgctcagctt cagaacgcgt accagttcag cgaatccgcg cacgaagggc
     6181 agttccgcaa gtccggcgat ccctatatct cccacccgat tgccgttgcc caaatcctgg
     6241 cgcagtggca tcttgacggc caggcactca ccgccgcgct gctccacgac gtgatggaag
     6301 acaccgccgt caccaagacc gagatcagcg acaagtttgg aaagcacgtc gctgatctcg
     6361 tggacggtgt ctccaagctc gaccggatcg agttccagac ccaggaagag gcacaggcgg
     6421 agaacttccg caagatgctg ctcgccatgg cgcgtgatgt gcgcgtcatc ctgatcaagc
     6481 tcgccgaccg cctgcacaac atgcgcacgc tgcaggtgat gcagccgaaa aagcgcgcgc
     6541 gcatcgcccg cgaaacgctg gaaatctacg cgccgatcgc caaccgcctt gggctcaacc
     6601 agctctacca ggagttgcag gagctggcgt tccgccacct ctacccgaac cgcttcaacg
     6661 ttctgtccaa ggcgctgctg cgcgcgcggg gaaatcgccg cgaggtggtg gacaggattc
     6721 tcgacgcgat caagcagaaa ctcgccgaca gttacatcga ggcgaccgtc agcggccggg
     6781 agaagaacct ctacagtatc tacaagaaga tgctggagaa gaacctgtcc ttcgcggaag
     6841 tgttcgacat ctacggattc cgcgtcatcg taaaggacgc cgctgcctgc tatctcgcgc
     6901 tgggcgcgct gcaccagctt tacaagccga tccccggcaa gttcaaggac tacatcgcca
     6961 tccccaaggg caacggctat cagtcattgc acaccacgct gttcggccct ttcggtacgc
     7021 cgatcgaggt gcagatccgg acgcttgaaa tgcacaagat cgccgaggcg ggcgtggctt
     7081 cccactggct gtacaaggac gatgcgcaga tcaacgaact gcacatgaag acccaccagt
     7141 ggctgcagag cctgctggag atgcagtcgg aaagcggcga ttcgatcgag tttctcgagc
     7201 acctcaaggt ggacctgttc cccgacgagg tctacgtgtt tacccccaag ggcaagatca
     7261 tggcgctgcc gcacggcgcc acctgcgtgg attttgccta cgcggtgcac accgacattg
     7321 gcaatcgctg tgtggccgcc aagatcaacc acgaactggt gccgttgcgc tcggaattga
     7381 aaagcggcga ccgcgtggag atcatcacgg ccgcccacgc caaccccaac ccggcctggc
     7441 tcaattacgt ggtaaccggc aaggcgcgct cgcacatccg ccattttcta aagaccatgc
     7501 agtacgagga gtcggcgcag ctcggcgaac gcctgctcag ccaggcgctg gccgcgctca
     7561 agtccagccc ggaccgaatc agccgtgacc aatgggagag gctgctcaag gaaaacagcg
     7621 ccaagacccg gcagcaggta ctggccgacc ttgggctggg caagcaactg gcgatcgtca
     7681 tcgcgcgcca gctgctgtcg atcgccgaac ccaaaggcag caagacggag cagctgcccg
     7741 gcagcctgat cattcgcggc tcggagggca tggcgctgca gtttgccaaa tgctgccacc
     7801 cgatacccgg tgatccgatc atcggtttcc tgcgcaaggg ccacggcctg gtgattcata
     7861 cccacgactg cccggtggcg cgcaagaccc gcggcgatcc ggaaaagtgg ctggacgtgg
     7921 agtgggcgac ggacaccaag aagctgttca ccgtaaacat caagatggtg gttgccaacg
     7981 agcgcggcgt gctggccaaa atcgccgcgg tgatcgccga gaccggctcg aatatcgaca
     8041 acgtcagcct tgagcccggc gattccagcg cctacaccat catccacttc accctccagg
     8101 tgtccaaccg cctgcatctg gcgcagatca tgcgcggcct gcggcgcgtt cccgaggtca
     8161 tccgcatctg gcgc
//
LOCUS       DASQOP010000031         4966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_212427, whole genome shotgun sequence.
ACCESSION   DASQOP010000031 DASQOP010000000
VERSION     DASQOP010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4966
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212427"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(248..2014)
                     /gene="sdhA"
                     /locus_tag="VE030_01105"
     CDS             complement(248..2014)
                     /gene="sdhA"
                     /locus_tag="VE030_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HZF22044.1"
                     /translation="MTIPVRKFDAVIVGAGGAGMRASLQLAEAGYKVAVLSKVFPTRS
                     HTVAAQGGIGAALGNMGEDHWMWHMYDTIKGSDWLGDQDAIEFLCREAPKVVIELEHF
                     GMPFDRNANGTVYQRPFGGHMQNFGAGPAVQRSCCAADRTGHAMLHTLYQRNVRASTR
                     FLVEWMALDLIRDVSGQVLGVTAMEMETGEVSVLHARAVLFATGGAGRIYSASTNAFI
                     NTGDGLGMAARAGIPLEDMEFWQFHPTGVAGVGVLITEGVRGEGGYLLNKNGERFMER
                     YAPNAKDLASRDVVARAMATEIKEGRGCGPDGDYLLLKLDHLGVELIESKLPGIREIA
                     RKFANVDPVRDPIPVVPTCHYQMGGIPANYRGEVVAPQGGDPEAVVPGFYAAGECACA
                     SVHGANRLGTNSLTDLLVFGKSSGDTMIRFLQQNPSHKDLPADASDRTVTRLARLDNQ
                     KDGESVHEVGAELRRVMQAHCGVFRFPDMLVEGVRKIKETASRVGRTGIKDKSRVFNT
                     ARVEALELDNLIEVALATLVSAEARKESRGAHDRADHHKRDDVNWLKHSLWYKESNRL
                     EYKPVHLKPLTAQTIEPKVRTY"
     gene            complement(2017..2361)
                     /gene="sdhD"
                     /locus_tag="VE030_01110"
     CDS             complement(2017..2361)
                     /gene="sdhD"
                     /locus_tag="VE030_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HZF22045.1"
                     /translation="MVKSVIGAHYGLGDWLVQRVTALLMAVYTIVFAGILLICPPQHY
                     GEWRALFENQPMRVATFLFFISLFWHAWIGVRDILMDYIKPTSVRLGLEILVAVLLVG
                     YAGWAIQILWSS"
     gene            complement(2355..2732)
                     /gene="sdhC"
                     /locus_tag="VE030_01115"
     CDS             complement(2355..2732)
                     /gene="sdhC"
                     /locus_tag="VE030_01115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HZF22046.1"
                     /translation="MIASPRPKFLDLTKIRLPVPGFVSILHRISGAALFLFAGVLLYL
                     FQESLASPESYVRFRALADHWLAKLFLTGMLWAFLHHFFAGLRYLLLDVDIGSELGTT
                     RAMSWAVLGTSLALTVVLGVCLW"
     gene            complement(2781..3515)
                     /locus_tag="VE030_01120"
     CDS             complement(2781..3515)
                     /locus_tag="VE030_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZF22047.1"
                     /translation="MKPTANAPAFQPLYRQIKSLITQSLVTGEWRPGEPIPSEIELAG
                     RFSVSQGTVRKAIGELAGENLLVRQQGKGTFVASHSEERSQFPYLRISPDRGALDSLA
                     ATLIDCRKVKLDSASAKKLDLASGAGAFLITRVLAISGRKAVYEEVRLPATRFKGLTL
                     ETIERHHCMLYSMYESEFEVRILHVDEQIKAVAAEGAATRHLGITKGAPLLTIERVAY
                     TYADKPVELRRSFCNTREHHYRNRIV"
     gene            3754..4740
                     /locus_tag="VE030_01125"
     CDS             3754..4740
                     /locus_tag="VE030_01125"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HZF22048.1"
                     /translation="MKSPLRVAVTGAAGQIGYSLLFRIASGEMLGKDQPVILQTLEIP
                     DEKAQAALKGVMMELEDCAFPLLAGMVAASDPKVAFKDADVALLVGARPRSKGMERKD
                     LLEANGAIFTGQGRALNEVASRKVKVLVVGNPANTNAYIAMNSARGLARENFTAMLRL
                     DHNRALSQLAAKTGKPVGSIEKMIVWGNHSPTMYPDYRFATIGGQPAPKVVNDEPWFR
                     NVFIPTVGKRGAAIIEARGLSSAASAANAAIDHVRDWLLGTNGKWVTMGIRSDGSYGI
                     PEGIIYGFPATCSAGRYQMVKGLEIDEFSRSKMDATLEELEEERDAIKHLLK"
BASE COUNT          960 a         1541 c         1517 g          948 t
ORIGIN      
        1 gcttggtgtc cttgtccggg tcgtagcggt agatactgaa gcgcatcttt cgtttcctca
       61 aaagttcatg ttatccgcca agacgccaag agcgccaaga aattcaaaag caaaacgtgt
      121 caaaaagcgg tgtattggca gtggcgcaag ttcccgtcga tttcgaatta gtccacaaca
      181 tcatgcatgt tgttcattct cgttatcgtt cttccttggc gctcttggcg ccttggcggc
      241 taaggtctta gtaagtccgt accttcggtt cgatcgtctg cgcggtcagc ggcttcaaat
      301 gcaccggctt atattcgagg cggttgcttt ccttgtacca caggctgtgc ttgagccagt
      361 tcacgtcgtc gcgcttgtga tggtcggcgc gatcgtgtgc gccccggctt tccttgcgcg
      421 cctcggccga gaccagcgtg gcgagcgcca cctcgatgag gttgtcgagc tcgagcgcct
      481 ctacgcgcgc ggtgttgaat accctgctct tgtccttgat cccggtgcgg ccgacgcgcg
      541 acgcggtttc ctttattttc ctgacaccct ccaccagcat gtccgggaag cggaacacgc
      601 cgcaatgcgc ctgcatgacc cggcgcaatt cggcgccgac ttcatgcacg ctctcgccgt
      661 ctttctgatt gtccaggcgc gccagcctgg tcaccgtcct gtccgatgca tcggccggaa
      721 gatccttgtg gctggggttc tgctgcagga aacggatcat ggtgtcgccg gaagattttc
      781 cgaacaccag cagatcggtg agcgaattgg ttccgagccg gttcgcgcca tgcaccgatg
      841 cacaggcgca ctcgcctgcc gcgtagaatc ccggcaccac cgcttccgga tccccaccct
      901 gcggcgccac cacctcgccg cggtaattgg cgggaatccc gcccatctgg tagtgacagg
      961 tcgggacaac cgggatcgga tccctgaccg gatcgacgtt ggcgaatttc ctggcgatct
     1021 cgcgaatgcc gggaagcttg ctctcgatca gttccacgcc caaatgatcg agcttgagca
     1081 gcaggtaatc gccgtcgggc ccgcagccgc ggccttcctt gatttcggtc gccatcgccc
     1141 tcgccaccac gtcgcggctg gccaggtctt tggcgttcgg cgcgtagcgc tccatgaaac
     1201 gctcgccgtt cttgttcagc aggtaaccgc cctcgccgcg tacgccttcg gtgatcagca
     1261 cgcccacgcc ggccacgccg gtcggatgga attgccagaa ttccatgtct tcgagcggaa
     1321 tcccggcgcg cgccgccatg cccaatccgt cgccggtgtt gatgaaggcg ttggtgctgg
     1381 cggagtagat ccgccctgca cctccggtgg cgaacagcac tgcgcgcgcg tgcagcaccg
     1441 acacctcgcc ggtctccatt tccattgcgg tcactcccag cacctgtccg gagacgtcgc
     1501 ggatcaggtc cagcgccatc cactcgacca gaaagcgggt gctggcgcgc acgttacgct
     1561 ggtacaacgt gtgcagcatc gcgtggccgg tgcggtccgc cgcacagcag gaacgctgca
     1621 ccgccgggcc ggcgccgaaa ttctgcatgt gcccgccgaa cgggcgctgg tacaccgtgc
     1681 cgttggcgtt gcggtcgaaa ggcatgccga agtgctcgag ctcgatgacc accttcggcg
     1741 cttcgcggca gaggaactcg atggcgtcct gatcgccaag ccagtccgac cccttgatgg
     1801 tgtcgtacat gtgccacatc caatggtctt cgcccatgtt gccgagcgcc gcgccgattc
     1861 cgccctgtgc cgctaccgtg tgcgagcggg tcggaaacac tttcgacagc acggcgacct
     1921 tgtaaccggc ctcggccagc tgcaacgacg cgcgcattcc ggcgccgccg gcaccgacga
     1981 tcaccgcatc gaattttctt accgggatcg tcattgctaa gaactccaga gaatctgaat
     2041 cgcccagccg gcgtaaccga ccaggagcac ggccaccagg atctccaggc cgagccgcac
     2101 cgacgtgggt ttgatgtagt ccatgaggat gtcgcgcaca ccgatccacg catgccagaa
     2161 aaggctgatg aagaacagga aggtcgcaac gcgcatcggc tgattctcga acagcgcgcg
     2221 ccactcgccg taatgctgcg gcgggcagat caggaggatg cctgcaaata cgatggtgta
     2281 aaccgccatg agcagcgcgg tgacgcgttg taccagccag tccccgagcc cgtagtgggc
     2341 gccgatgacg ctcttcacca taggcacacc cccaggacta cggtcagcgc gagacttgtg
     2401 cccagcacgg cccagctcat cgcccgcgtg gtcccgagtt cggagccgat gtccacatcg
     2461 agcaacaagt agcgcagccc ggcgaagaaa tggtgcagga aagcccacag catgccggtc
     2521 aggaacagct tcgccagcca atggtcggca agcgcgcgga atcgcacata gctctccggc
     2581 gaagccagac tctcctggaa cagatacagc agcacacccg cgaacaggaa cagcgcggcg
     2641 ccgctgatgc gatgcaggat tgatacgaaa cccggcaccg gcaggcggat tttcgtgagg
     2701 tcgaggaatt tcggtcgtgg gctggcgatc atcagtaggt cctggtggcc agacgcgcag
     2761 cgggaagcgg gcgccggcgg ctagacgata cggttgcggt aatggtgctc ccgggtgttg
     2821 cagaaactgc ggcgaagctc gaccggcttg tccgcgtaag tataggccac gcgctctatc
     2881 gtcagtaacg gcgcgccttt ggtaatcccg aggtgcctgg ttgcggcccc ttccgcggcg
     2941 acggccttga tctgctcatc gacgtgcagg atgcggactt cgaactcgga ctcgtacatg
     3001 ctgtagagca tgcagtggtg gcgctcgatg gtttccagcg tcagtccctt gaatcgtgtc
     3061 gccggcaggc ggacctcctc gtacaccgcc ttcctgccgg aaattgccag cacgcgcgta
     3121 atcagaaatg cgccggcgcc ggacgcaaga tcgagcttct tcgcagaggc gctgtccagt
     3181 ttgaccttgc ggcagtcgat cagggtcgct gcgagcgagt cgagggcgcc gcggtccggc
     3241 gatatgcgca ggtagggaaa ctggctgcgc tcctcggaat gggatgcgac aaaagtgccc
     3301 ttgccctgct ggcgcaccag cagattttcg ccggcaagtt cgccgatggc cttgcgcacc
     3361 gtgccctggc tgacgctgaa gcgtccggcc agctcgattt cgctgggaat cggttcgccc
     3421 ggacgccact cgcccgttac caggctctga gtgatcagac tcttgatctg ccggtaaagc
     3481 ggttgaaagg cgggcgcgtt ggcggtgggc ttcatgttgc cgcgcgtttt tagcacgatt
     3541 ttccttgggc cgtccacgag aacagatgtc ttttataaga tacaagactt gtttgacatt
     3601 gtcgaatccg aatcgctatg atgcagggca aaaataaccc gggctgagcg cttgaccggg
     3661 catccggccg gactcgtcag gcgtactcgt caagcggcgt aaaagactga tttatttgca
     3721 agaagccctc acctcacgca ggagtctgca gccatgaaat cgcccctccg agtcgcagtc
     3781 accggtgccg caggccagat cggatacagc ctcttgttcc gcattgccag cggggaaatg
     3841 ctgggcaagg accagccggt cattctgcag acgctcgaga tcccggacga gaaggcgcag
     3901 gcggcgctca agggagtgat gatggaattg gaggactgcg cgttcccgtt gctggccggc
     3961 atggtcgcgg cctccgatcc gaaagtcgcg ttcaaggacg ccgacgtggc gctgctggta
     4021 ggcgcccggc cccgttccaa gggcatggaa cgcaaggacc tgctggaagc caacggcgcg
     4081 atcttcaccg gacagggccg ggcactgaac gaagtagcca gccgcaaagt gaaagtgctg
     4141 gtggtgggca acccggccaa taccaacgcc tatatcgcga tgaacagcgc gcgcggcctg
     4201 gcgcgcgaga actttaccgc gatgctgcgt ctggaccaca accgcgcgct gtcgcagctt
     4261 gcggccaaga ccggcaagcc ggtcggttcg atcgagaaaa tgatcgtgtg gggaaaccac
     4321 tcccctacca tgtaccctga ctaccgtttc gccaccatcg gcggccagcc ggcccccaag
     4381 gtcgtcaatg atgaaccgtg gttccgcaac gtcttcattc cgacggtggg caaacgcggc
     4441 gccgcgatca tagaggcgcg cgggctctcg tccgccgcct ccgcggccaa tgccgcgatc
     4501 gaccacgtgc gcgactggtt gctcggcacc aacggcaaat gggtcaccat gggcatccgc
     4561 tccgatggtt cgtacgggat tcccgagggc ataatctacg gttttccggc gacctgttcc
     4621 gcgggaagat atcagatggt caagggcctg gagatcgacg agttcagccg cagcaagatg
     4681 gacgccacgc tcgaggaact ggaagaagag cgcgacgcga tcaagcacct gctcaaatga
     4741 aaggtcgggc gagcgttatt caagaaaccg aatctcacca cagagacaca gagacacaga
     4801 gacacagaga cacggagagc ggcaaaggca aggacgttta ggataggaag gccacacaaa
     4861 tcatgcggca ccgcattctc tttgcttcat gtgaccatac ttttcttcgg tttgaatttc
     4921 tctgtgcctc cgtgtctctg tggttgatgg attgggagat ataacg
//
LOCUS       DASQOP010000032         6676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_817440, whole genome shotgun sequence.
ACCESSION   DASQOP010000032 DASQOP010000000
VERSION     DASQOP010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6676
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817440"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            428..1156
                     /locus_tag="VE030_01130"
     CDS             428..1156
                     /locus_tag="VE030_01130"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZF22049.1"
                     /translation="MRFINLAGYKFVPLERPPELKASLQEFCGELDLKGTILLSPEGI
                     NIFVSGSGPAVTRFKAFLHADPRLAGLRFKESVSSYQPFKRMLVKIKKEIITLRVAGL
                     DAAAKPAPPVMPAELKRWLDEGREVVLLDIRNAFEVETGTFRDAVHLGLKSFGEFPRA
                     ADRLDVSLKGKTIVAFCTGGIRCEKAAPVLIQKGFRSVFQLEGGILQYFKDCGDAHFK
                     GRCFVFDQRTALDGNLNPRPASAK"
     gene            1153..2064
                     /locus_tag="VE030_01135"
     CDS             1153..2064
                     /locus_tag="VE030_01135"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZF22050.1"
                     /translation="MTGAHETRRAVIAMILLVILWGYSWITSKVGLFYASPLDFAAIR
                     VELGVLTLFIGLLWRRSSLRPQHWPALILIGLIQTFAFIILNTWALSEGGPGKTSVLV
                     FTMPFWVLVFAWPVLHERIHGLQWVSVVLAAIGLVFVLEPWQMHTSMFSKTLAVLAGM
                     CWAIGVVYAKRLHNRAPVDVLSFTFWQMLVGLIPILIAQVAFDRDPVNWTGTFIFAAV
                     FNGVLATGIGWLTWLYVLQRLPAGTTSLSSLGIPLIAIVASWLQFGEEPGKSELIGML
                     LIGIALAILSYLSIRRHEEVEPMTGQE"
     gene            2275..3141
                     /locus_tag="VE030_01140"
     CDS             2275..3141
                     /locus_tag="VE030_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZF22051.1"
                     /translation="MKLSQSQFLNIRGLRYHVRTWGSESAPKLFFLHGWMDVSASFQF
                     AVDALKHDWFVIAPDWRGFGLSEWNNDSYWFPDYIADLDALLRHFQPDAPVYVVSHSM
                     GGNVANLYAGIRPERVGKLVLAEGFGLPPAKPEKAPERYARWLDECRQPPTLRPYSTF
                     AEVIQRLKTNNPRITDERAAFLAPHWAGQMPDGSIRLRADPCHKMVNPVLYRVEEAMA
                     CWRRITGPTLWLWGDGEWMMQWMKGDKSLLDSYRSCYRSLQELTIADAGHMMHHDQPE
                     RFAHAVESFLLA"
     gene            3234..3680
                     /locus_tag="VE030_01145"
     CDS             3234..3680
                     /locus_tag="VE030_01145"
                     /inference="COORDINATES: protein motif:HMM:NF019932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZF22052.1"
                     /translation="MNRATWYRLFSGLLLAGSGLLAGPALAGPVEEIHAVEDRRYDAM
                     IRGDADALANTLAEEFAYHQPNGKTNDKPGYIKQMQSGAVKIFSAERYDVKVNLYGDV
                     ATAMGSTRLDVEIAGERRKADLRYLNVWVKRDDRWQLAARQSAFTK"
     gene            complement(3684..4880)
                     /locus_tag="VE030_01150"
     CDS             complement(3684..4880)
                     /locus_tag="VE030_01150"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZF22053.1"
                     /translation="MKGVVWPLIATMAIQALVSMAVFAPPVIAPAAHDDIGFPASWVG
                     IVTALTFAAATFGALFSGGVIARVGALRMSQLSLLLCGSGIALIASSNAWLVALGALV
                     MGLGYGPVTPSSSAILADRTPERIRSFIFSLKQTGVPIGGALAGAIVPTLIVAFGWRT
                     SALSVGLACVALAAAIQPYRAVVDLAPHVDRPKRPASLLEPLRLVLAHPRLREMAFAS
                     CTYSGMQACLSTFLVVYLTQRVGFSVPVAGAVLATAMTAGIIGRISWGVVADNGVRPR
                     ALLGILGVTMSVGAFLTATFTGTWPIAAVFAVSFAYGATAIGWNGVYLSEVARIAPPG
                     KAGAATGASLAMTYAGVVALPTLFWLIVILGNSYAAAFAAAGGLTLWRGSYFFRFDGQ
                     APPDSP"
     gene            complement(5099..6385)
                     /locus_tag="VE030_01155"
     CDS             complement(5099..6385)
                     /locus_tag="VE030_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HZF22054.1"
                     /translation="MADREFGFGTLCLHAGQIPDAATGSRAVPIYQTTSFVFDSAEHA
                     ASLFNLQTFGNVYSRISNPTVAVFEERMAALEGGRAALAASSGQAAQMCALLTILREG
                     DEIVSASTLYGGTYSQFDVTFRQFGINTVFVNPDDPENFRKALTPRTRVLYAETMGNP
                     QINVLDIEVCAKIAKEADVPLIVDNTFASPYLCQPIRFGADIVIHSATKFIGGHGTTM
                     GGVVVESGKFPWGNGKFPMMTEPSRGYHGVIFHETFGDFGYTMKCRMETMRTLGPVLS
                     PFNAWMLLQGLETLHVRMERHVANAVAVAKFLENHARVSWVNYPGLPSSKYHALAQKY
                     LPKGTGSIMTFGIKGGMEAGVKFIEALQFCSHLANVGDSKTLVIHPASTTHRQLSEEE
                     QVKAGVGPDMIRLSIGIEDVDDILWDIDQALSQSRT"
     gene            complement(6508..>6676)
                     /locus_tag="VE030_01160"
     CDS             complement(6508..>6676)
                     /locus_tag="VE030_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HZF22055.1"
                     /translation="GVMNEKAALKARKAGLEVVQDRCVKIEHGRLFGGLNWVGVNTRI
                     ISAKRPQWLPY"
BASE COUNT         1317 a         2066 c         2009 g         1284 t
ORIGIN      
        1 tcggtcaacg ccagcgcggg catcgaatcc gcctttgctg tcgccactgc gccgtcgagc
       61 cgggcgatgc cgtcaacgat cgaatattcg ctgtgcaagc gcagatggat gaacttcggt
      121 tgcatgagcg gcattttacg cgctcgttga aagactggcg caggactcgc cacaaagcct
      181 taaccacgga ggacacagag gacacggaag aaaccaagga caaaaatcgg gagtcagaac
      241 aagggaaatc aaggaggcca tccggatggt atgggtgttt gagccgacaa tccgttcctt
      301 gaaatcacgt aagtttttat tctgtttttg cttttcctcc gtgtcctctg tgtcctccgt
      361 ggtgagggat ttttttgttt gcgtcccggc ttgctaaaat acgcgccttt ccatcgccac
      421 ccctgccatg cgcttcatca atctcgccgg ctacaagttc gtgccgctcg aacgtccacc
      481 cgaactcaaa gccagcctcc aggaattctg cggggaactc gacctcaagg gcacgatcct
      541 gctaagcccc gaaggcatca acatatttgt ctccggctcc gggccagcgg tgacccgctt
      601 caaggctttc ctgcacgccg atccccgcct cgccggcctg cgcttcaagg aaagcgtttc
      661 ctcgtaccag cctttcaaac gcatgctggt caagatcaag aaagaaatca tcactctgcg
      721 cgtggccggc ctcgacgcgg cggcaaagcc cgcgccgccc gtaatgccgg ccgagctcaa
      781 gcgctggctg gacgaagggc gcgaagttgt cctgctcgac atccgcaacg cgttcgaagt
      841 ggaaacaggc acattccgcg acgccgttca cctgggcctt aagtcgttcg gtgagttccc
      901 gcgtgcggcg gacaggctcg atgtatcgct caagggcaag actatcgttg ccttctgcac
      961 cggcggcatc cgctgcgaga aggccgcgcc ggtgctcatt caaaagggat ttcgcagcgt
     1021 cttccagctt gaaggcggca tcctgcagta cttcaaggat tgcggagacg cgcacttcaa
     1081 aggccgctgc tttgtcttcg atcagcgtac tgcgctggac ggaaatctga atccgaggcc
     1141 cgcgagcgcc aaatgaccgg ggcacacgaa actcgcaggg cagtgatcgc gatgatcctg
     1201 ctggtgatcc tgtggggtta cagctggatc acctccaagg tcggcctgtt ttacgccagt
     1261 ccgctcgact tcgccgcaat tcgcgtcgaa cttggcgtgc tgacactgtt catcggcctg
     1321 ctgtggaggc gatcgtcgct ccggccacag cattggccgg cgctcattct catcgggctg
     1381 atacagactt tcgccttcat cattctcaac acctgggcgc tgtccgaagg cggccccgga
     1441 aagacctcgg tgctggtttt caccatgccg ttctgggtgc tggtgttcgc ctggccggta
     1501 ctgcacgaac gcatccatgg tttgcagtgg gtatcggtcg tgctggcggc gattgggctg
     1561 gtgttcgtgc tcgaaccctg gcaaatgcac acttcgatgt tcagcaagac cctggcggtg
     1621 ctggccggca tgtgctgggc catcggcgtg gtgtatgcga agcgcctgca taaccgcgca
     1681 ccggtggacg tactgtcgtt caccttctgg cagatgctgg tggggctgat tccgatcctg
     1741 atcgctcaag tcgccttcga ccgtgatccg gtcaactgga ccggtacttt catctttgcc
     1801 gccgtgttca acggcgtgct cgccactggc atcggctggc tgacatggct gtacgtactg
     1861 cagcggctgc ccgcgggcac aaccagcctg tcatcgctgg ggataccgct gatcgcgatt
     1921 gttgcctcgt ggctgcagtt cggcgaagaa ccgggcaagt ccgaactcat cggcatgctg
     1981 ttgatcggca tcgcgctcgc gatcctgtcc tacctcagca tccgcaggca cgaagaagtc
     2041 gaacccatga ccggccagga gtagcgataa gcgctcaccg cagaggacgc ggaggaaaag
     2101 caagagcaag aaaacaagtt ggggtgaaaa catttcgttg gctcatgatc cattgcagcg
     2161 aacattggcg gcacaggtta gcgaacaggt acctcgcttt tatccccaat ttgtttttcc
     2221 tcgttttcgt tcttcctccg cgtcctccgc gtcctctgcg gtaagagttt gactatgaaa
     2281 ctcagccaat cgcagttttt gaacatccgc ggcctccgct accacgtgcg cacctggggc
     2341 agcgaatccg cgccgaaact tttcttcttg cacggctgga tggacgtgtc ggcgtcgttc
     2401 cagttcgcgg tggatgcgct caagcacgat tggttcgtga tcgcgccgga ctggcgtggc
     2461 ttcggtctgt cggagtggaa caacgatagt tactggttcc cggattacat cgccgatctc
     2521 gacgcgctgc tgcgccattt ccagcccgac gcgccggtct atgtcgtcag ccacagcatg
     2581 ggcggtaacg ttgcgaacct ctacgccggc atccgccccg agcgcgtcgg caagctcgtg
     2641 ctggcagagg gcttcgggct gccgccggcc aagccggaaa aagcgcccga acgttatgcc
     2701 cggtggctgg acgaatgccg ccagccgcca actttgcgcc cctactcgac gttcgcggaa
     2761 gtcattcagc ggctcaagac caacaatcca cgcatcaccg acgagcgcgc cgcattcctc
     2821 gcgccgcact gggccggcca gatgcccgac gggagcatcc gactgcgcgc cgatccctgc
     2881 cacaagatgg tgaatccggt gctataccga gtcgaggaag ccatggcctg ctggcggcgc
     2941 attaccggcc caacgctctg gctgtggggc gacggcgaat ggatgatgca gtggatgaag
     3001 ggcgacaagt cgttactgga cagctaccgg tcctgctatc gatccctgca ggagctaacc
     3061 attgcggatg ccggccatat gatgcatcac gaccaacccg agcgattcgc gcacgcggtt
     3121 gaatctttcc tgctggcctg aatctccgcc ccgccatcga acttgaaggt ctagaattcg
     3181 cagcggagcc cggtcgtcgg gccgataaca acttcaatcg ggaggaatcg gatatgaacc
     3241 gcgcgacgtg gtacaggctg ttctcgggac tcttgctcgc cggatcgggt ctgctcgcgg
     3301 ggcccgcgct cgccggacct gtggaggaga tccacgcagt cgaggatcgt cgttacgatg
     3361 cgatgattcg cggcgatgcc gatgcattgg cgaatacgct ggccgaggag tttgcctacc
     3421 atcaacccaa cggcaagacc aacgacaaac cgggctacat caaacagatg cagagcggcg
     3481 ccgtgaaaat cttcagcgcc gagcgctatg acgtcaaggt caacctctat ggcgatgtcg
     3541 ccacggcaat gggctcaacg cggctggacg tcgagattgc cggcgaacgc cgcaaggccg
     3601 acctgcgcta tctgaacgtc tgggtcaaac gcgacgaccg ctggcaactg gccgcacggc
     3661 agtcggcatt cacgaaatag gccttatggc gagtccggcg gggcttgacc gtcgaagcga
     3721 aaaaagtagg agccgcgcca aagcgtaagg ccgcccgcgg cggcgaacgc agcggcataa
     3781 ctgtttccca atattacgat cagccagaac aaagtcggca gcgccaccac gcccgcataa
     3841 gtcatcgcca gcgaagcccc ggtcgcggcg ccggccttgc cgggcggcgc gatgcgggcg
     3901 acttccgaga gatacacccc gttccatccg atcgcagtcg caccgtaagc gaaactcact
     3961 gcaaatacgg cggcgatcgg ccacgtgccg gtgaacgtcg cagtgagaaa agctcccaca
     4021 gacatcgtca cgccgagaat tccgagcagc gcccgcggtc tcaccccgtt gtccgccacg
     4081 actccccagg aaatgcgacc gatgatgcct gccgtcatcg cggtcgccag caccgctccg
     4141 gcgaccggca ctgaaaatcc gactcgctgg gtcagataga cgacgaggaa ggtgctgagg
     4201 caggcctgca tcccggaata ggtacaggac gcgaaagcca tttcgcgcag gcgcggatgc
     4261 gcgagcacca ggcgtagcgg ctcgagcagg cttgccggcc ttttcggacg atcgacatgt
     4321 ggcgcgagat ccaccacggc ccggtagggc tggatcgctg cggccagcgc gacacacgcc
     4381 agacccaccg acaatgcgga tgtcctccag ccgaaagcga cgatcaacgt gggcacgatc
     4441 gctccggcaa gcgctccgcc gatcggcacg ccggtctgct tcaacgagaa gatgaagctg
     4501 cggatgcgct cgggtgtgcg gtccgcgagg atcgccgaac tggaaggcgt caccggccca
     4561 taccccaggc ccatcaccag cgcaccgagc gcgaccagcc aggcgttgga gctcgcgatc
     4621 agcgcgatac cgctgccgca cagcagcagc gagagctggc tcattcgcag cgcgccaacc
     4681 cgggcgatga cccctccgga aaagagcgcc ccgaaagtgg ctgcggcgaa ggtcagggca
     4741 gtgacgatcc cgacccagct cgcggggaat ccgatgtcat cgtgtgcggc cggggcgatt
     4801 accggcggcg cgaacaccgc catcgacacc aatgcctgga tggccatggt tgcgatcaac
     4861 ggccacacta cgcctttcat ttagaatcca aattttcacc gcaaagacgc agagggcaca
     4921 gagaaaagcg cagaaaagaa gaatgaattg ctgtcttgat gtgagcaggt atcggaaatc
     4981 taccgtctgc cttgcggcac ggcgttccca tcgtgtggag aatcattgcc tctgaccccc
     5041 agtccattgc ttttccttcg cgttctttgc gtctttgcgg tgagaacagc attagaagtc
     5101 aggttctgga ctgggagagg gcctgatcga tgtcccagag aatgtcgtcg acgtcctcga
     5161 tgccgatgga caggcggatc atgtcgggcc cgaccccggc cttgacctgt tcttcctccg
     5221 acaattgccg atgagtggtg gaagccggat ggatcaccag cgtcttggag tcgccgacgt
     5281 tggcaaggtg cgagcagaac tgcagcgcct cgatgaactt cacccccgct tccatgccgc
     5341 ctttgatgcc gaaggtcatg atcgagccgg tgcccttggg caggtatttc tgcgccaggg
     5401 cgtgatactt gctgctcggc agcccggggt aattcaccca ggagacccgc gcatggttct
     5461 cgaggaattt cgccaccgcc accgcgttgg cgacatggcg ctccatgcgc acatgcaatg
     5521 tctccagccc ctgcagcagc atccaggcgt tgaacggcga caacactggg ccaagcgtgc
     5581 gcatggtttc catccggcac ttcatggtgt agccgaagtc gccgaaggtc tcgtggaaga
     5641 tcacgccgtg atagccgcgc gagggctcgg tcatcatcgg gaacttgccg ttgccccatg
     5701 gaaacttgcc ggattcgacc accacgccgc ccatggtggt gccgtggccg ccgatgaact
     5761 tggtcgccga atggatgacg atgtcggcgc cgaacctgat gggctggcac aggtacggcg
     5821 aggcgaaggt attgtcgacg atcagcggca cgtccgcttc ttttgcaatc ttcgcgcaga
     5881 cctcgatgtc gagcacgttg atctgcgggt tgcccatggt ttccgcgtag agcacgcgcg
     5941 tcctcggggt gagcgccttg cggaaattct ccgggtcgtc cgggttcacg aacacggtgt
     6001 tgatgccgaa ctggcggaaa gtcacgtcga actgcgaata ggtgcctccg tagagcgtgc
     6061 tcgccgagac gatttcgtcg ccttcgcgca ggatggtcag cagggcgcac atctgcgcgg
     6121 cctggccgga ggaagcggcc agcgccgcac ggccgccttc cagtgccgcc atgcgctcct
     6181 cgaacaccgc caccgtcgga ttcgagatcc gcgaatagac gttgccgaag gtctgcaggt
     6241 tgaacaggct cgccgcgtgc tcggccgagt cgaacacgaa cgaagtggtc tggtaaatcg
     6301 gcaccgcgcg cgaacccgtg gccgcgtccg ggatttgtcc cgcgtgcagg cacaaggtac
     6361 cgaatccgaa ttctctatct gccatgtgaa tgccgtgagg ggtgaagggt gaagggtaaa
     6421 aggcgactag gtgattgagc gattaagtga ttgggggatt cgatccgcac ttaccattca
     6481 cttttcacca ttcactattc accgcttcta atacggcagc cactgaggcc gctttgcgga
     6541 gatgatcctg gtgttgacgc ccacccagtt cagccccccg aacaaacgcc cgtgttcgat
     6601 cttgacgcag cgatcctgca ccacttcgag cccggccttg cgcgccttca gcgccgcctt
     6661 ttcgttcatc acgccg
//
LOCUS       DASQOP010000033         3860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2428751, whole genome shotgun sequence.
ACCESSION   DASQOP010000033 DASQOP010000000
VERSION     DASQOP010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2428751"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VE030_01165"
     CDS             <1..245
                     /locus_tag="VE030_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010393974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HZF22056.1"
                     /translation="MSYLKPDVQRSGLDQERFYALGIDAYRIVQELLRPYEPMVSLDG
                     VTGTITPTDTQQFTRSLVPAQFSQGRAKPVEAPTMR"
     gene            242..583
                     /locus_tag="VE030_01170"
     CDS             242..583
                     /locus_tag="VE030_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HZF22057.1"
                     /translation="MSNHGTWAEDAAAAFLVKRGLKLLERNYRCRFGEIDLVLRDART
                     LVFVEVRYRSSHSFGGAGESITTSKRKKLLRAARHYMAAHGEFPACRFDAVLLDGDTP
                     EVDWVVDAFGE"
     gene            658..1248
                     /locus_tag="VE030_01175"
     CDS             658..1248
                     /locus_tag="VE030_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HZF22058.1"
                     /translation="MDLKSRINKHFTDSAHLKLEAMDVLAEPLAHASERMAHCLMNEG
                     KILSCGNGGSAADAQHFAAELLNRFETERPPLAAMALSTDTSTLTSIANDYSYNDVFS
                     KQVRALGQSGDLLLAISTSGNSANVMEAIHAAHDRQMGVIALTGKKGGKMAGVLGPND
                     VHICVPADRTARIQEVHLLCLHCMCDAIDCLLLGVE"
     gene            1249..1824
                     /locus_tag="VE030_01180"
     CDS             1249..1824
                     /locus_tag="VE030_01180"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HZF22059.1"
                     /translation="MKLPIRFIATILALSPLLQSCLPLIVGAGVGAGVMIADDRRTSA
                     TVLEDQTIELKIKNRINEKYDDQLYVNVTSFNRFVLLTGEAPTEEIKQDLPILALEVQ
                     NVRNVQNEVIVAGNSSFTSRSSDALLTSRIKGRLTQNKDVSSNHVKVVTENGTVFLMG
                     LVTRAEAESAAQTAATTSGAQRVVKVFEYID"
     gene            1967..2452
                     /locus_tag="VE030_01185"
     CDS             1967..2452
                     /locus_tag="VE030_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012358505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HZF22060.1"
                     /translation="MAHRPSFDKKICPPGKLPVRVSALSRPLVFTNGVFDMLHRGHAT
                     YLAESRALGSSLLVALNADASVKRLGKGPDRPINTLEDRLAVVAALESVSLATWFEED
                     TPLELILVCRPDVLVKGGDWPVGKIVGGREVADWGGKVVSIPFRYERSTTSLISRIRK
                     P"
     gene            2481..>3860
                     /locus_tag="VE030_01190"
     CDS             2481..>3860
                     /locus_tag="VE030_01190"
                     /inference="COORDINATES: protein motif:HMM:NF017775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HZF22061.1"
                     /translation="MSARPRFVRDTWGLIKPYWSSEERYSAWLLLAAVIGLTLAMVFM
                     NVQFNSWYNDFYNALQEKNKEEFFRLMIRFAILAAIYITMAVYAFYLNQMLQIRWRRW
                     MTDVYLQRWLAERTYYRMQLTGSPADNPDQRIAEDFKLFVDETLSLSLGFLNAVVTLG
                     SFVGILWGLSGPLAIPYNGGEIVVPGYMVWAALLYAIVGTSLTHKIGKALIGLNFNQQ
                     RYEADFRFGLIRFRENSEGVALYNGEEDESRNFRSRFANVVDNWWRIMKRQKVLNSFT
                     IGYNQLAVIFPFVVAGNRYFAGAIQLGGLMQISNAFGQVQSSLSWFINAYSGNAPSTG
                     FVGWKATTDRLLGFHYAIEKAREETQQKSGVQQAQDGGEDLVIDNVALSLPNGQLLIS
                     ASNAIIKSGESLLICGPSGSGKSTLFRAIAGIWPFGKGRMRLPAGFRVLFLPQRPYLP
                     IGTIRDVVSY"
BASE COUNT          782 a         1187 c         1119 g          772 t
ORIGIN      
        1 tgatgagtta tctgaagccc gacgtgcaaa ggtcggggct cgatcaggaa cggttctacg
       61 cgcttggaat agacgcatac cggatcgtgc aggaattgtt gcgcccgtac gagccgatgg
      121 tttctctgga cggtgtaacc ggcacgataa cgcccaccga cacccagcaa tttacccgct
      181 cactggtacc ggcgcagttc tcccaaggcc gcgccaaacc ggttgaagcg cccactatgc
      241 gatgagcaat cacggaactt gggccgaaga tgccgctgcg gctttcctgg taaagcgcgg
      301 tctcaagctt ctggagcgca actaccgctg tcgtttcggc gaaatcgatc tggtactgcg
      361 cgacgcccgg acactggttt tcgtcgaagt gcgataccgc agtagccact ccttcggcgg
      421 ggccggcgag agcattacca ctagcaagcg caaaaaactc ctgcgcgcgg cccgacatta
      481 catggcggcg cacggcgagt tcccggcctg ccgtttcgat gcagtgttgc tggacggcga
      541 caccccggag gtcgactggg tcgtggacgc attcggagag tgaacatcag cagcagcggc
      601 ttcgcacgtg ctttgtgcaa taattcgccc gtaacgggaa cgcgggctat tttcaggatg
      661 gacctcaaga gccggatcaa caagcatttc accgacagcg cccacttgaa actggaggcg
      721 atggatgtgc ttgccgagcc gctcgcgcac gcctccgaac gcatggcgca ttgcctgatg
      781 aacgaaggca agatcctgtc ctgcggcaac ggcggctccg ccgccgatgc ccagcacttc
      841 gcggccgagt tgctgaaccg cttcgagact gagcgcccgc ctctcgcggc gatggcgctt
      901 tccaccgata cttccacgct cacttcgatc gccaacgact acagctacaa cgacgttttc
      961 tcgaagcagg tgcgcgcgct tggccaatcc ggggacctgt tgctggcgat ttccaccagc
     1021 ggcaactcag ccaatgtcat ggaagcgata catgctgctc acgaccggca gatgggcgtg
     1081 atcgcactga ccggcaagaa aggcggaaag atggccggag tgctcggccc gaacgacgtg
     1141 catatctgcg tgcccgcgga cagaacggcg cgcatccagg aagtccacct gttgtgcctg
     1201 cattgcatgt gcgacgccat cgattgtttg ttgctaggag tggaatgaat gaaactacca
     1261 atccgattca tcgcgacgat tcttgccctt tctcccctct tgcagtcttg ccttccgctg
     1321 atcgtcggtg ccggtgtggg tgccggcgtc atgattgccg atgaccgtcg taccagcgcc
     1381 acggttctcg aggaccagac catcgaactc aagatcaaga accgcatcaa cgaaaagtac
     1441 gacgatcagc tttatgttaa cgttaccagc ttcaatcgct tcgtgctgct cactggcgaa
     1501 gctcccacag aggagatcaa gcaggatctg cccatccttg ctctggaagt gcagaacgtg
     1561 cgcaacgtgc agaatgaagt gatcgtggcc ggaaatagct cattcacttc ccgcagcagc
     1621 gatgcactgc tcaccagccg catcaagggg cggctgacgc agaacaagga cgtcagctcg
     1681 aaccatgtga aggtcgtgac cgagaacggc accgtgtttc tcatggggct cgtgacccgg
     1741 gccgaagccg agtccgcggc gcagaccgcc gccaccacca gcggcgcgca gagagtggtc
     1801 aaggttttcg aatacatcga ctgaggttcc ggtagaacag caatgaagaa agtccctcgc
     1861 ggggcttttt tcattttggc cacggccggt caactctttc agcctgtccg cggcgagcac
     1921 ggccccttca cccacctgta tcatggcgcc acctggaaca ggcaccatgg cccaccgccc
     1981 ttccttcgat aaaaaaatat gcccgcctgg caaactgccc gtgcgggtaa gcgcgctgtc
     2041 gcgcccgctg gtgtttacca acggcgtctt cgatatgctg caccgcgggc acgcaactta
     2101 ccttgccgaa tcgcgggcac tcggctccag cttgctcgtg gcgctcaacg cggacgcatc
     2161 ggtgaaacgg ctgggcaagg ggccggaccg gccgatcaat acgctggaag accgcctggc
     2221 ggtggtggcc gcgctggaaa gcgtgagcct cgccacatgg ttcgaggagg acaccccgct
     2281 cgagctcata ctcgtttgcc gccccgatgt cctggtcaag ggtggcgact ggccggttgg
     2341 taaaatcgtg ggcggcaggg aagtggccga ctggggcggt aaggtcgtat ccattccgtt
     2401 ccggtacgaa cgttccacca catcgctgat ctcccgcatt cgcaaaccct gactcgtcat
     2461 cttttctgaa ggaagcttcc atgtctgcca gaccgcgttt cgtgcgtgac acgtggggac
     2521 tgatcaagcc ttactggtcc tccgaggagc gctactccgc ctggttattg ctcgccgcgg
     2581 tcatcggcct gacactcgcc atggtcttca tgaacgtcca gttcaactcc tggtacaacg
     2641 acttctacaa cgcgctgcag gaaaagaaca aggaggagtt cttccgcttg atgatccgtt
     2701 tcgcgatcct cgcggccatc tatatcacga tggcggttta cgccttctac ctgaaccaga
     2761 tgctgcagat ccgctggcgg cgctggatga ccgacgtgta cctccagcgc tggctcgccg
     2821 agcgcaccta ttaccgcatg cagctcaccg gcagcccggc ggataacccc gaccagcgaa
     2881 tcgccgagga cttcaagttg ttcgtggacg agaccctaag cctttcgctc ggctttctca
     2941 acgccgtggt cacgctgggc tccttcgtcg gaatcctgtg gggcctatcc ggtccgctag
     3001 cgattcccta caacggcgga gaaatcgtcg taccgggcta catggtttgg gcggcgctgt
     3061 tgtacgcgat cgtcggtact tcgctaacgc acaagatcgg caaggcgctg atagggctga
     3121 atttcaacca gcagcgctac gaggcggatt tccgcttcgg cctgatacgg ttccgtgaaa
     3181 acagcgaggg cgtggcgctc tacaacggcg aggaagacga atccaggaat ttccgctcgc
     3241 gcttcgccaa cgtagtcgac aactggtggc gcatcatgaa gcgccagaag gtcctcaact
     3301 ctttcaccat cggctataac cagctggcgg tgatcttccc gttcgtcgtc gccggcaacc
     3361 gctattttgc cggcgccatc cagctcggcg gtctgatgca gatttccaac gcattcgggc
     3421 aggtgcaaag ctcactttcg tggttcatca atgcttactc cggtaacgcc cctagtaccg
     3481 gttttgttgg gtggaaggcg acaacagacc gtctgctggg tttccactat gcgatcgaga
     3541 aagcgcgcga agaaacgcag cagaaatccg gggtgcaaca ggcgcaagat ggcggcgaag
     3601 accttgtcat cgacaacgtt gcgctcagcc tgcccaacgg ccaactgctg atttcagctt
     3661 cgaacgcgat catcaaatct ggcgaaagcc tgctgatctg cggaccctcc ggctccggaa
     3721 agagcaccct gttccgggcg atagccggga tctggccatt tggaaaaggt cgcatgcgtc
     3781 tgccggcggg cttccgtgtc ctgttcctgc cgcagcggcc ctacctgccg atcggcacga
     3841 ttcgcgacgt cgtaagctac
//
LOCUS       DASQOP010000034        14095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2630807, whole genome shotgun sequence.
ACCESSION   DASQOP010000034 DASQOP010000000
VERSION     DASQOP010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14095
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2630807"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            310..1287
                     /locus_tag="VE030_01195"
     CDS             310..1287
                     /locus_tag="VE030_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018444358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HZF22062.1"
                     /translation="MPPENAEPKDAPDMPWQPSQRYPDSAVQIIDQSFTRYRLNLAAV
                     ERIAHGFRWSEGPVWFGDGRYLLWSDIPNNRIMKWEEETGAVSIFRRPSTFANGNTRD
                     RQGRLVTCEHGGRRVTRTEYDGTISVIAGAFGGKPLNSPNDVIVKSDGSIWFTDPPFG
                     LLGYYEGHLAKQELPMNVYCVDGQSGEMTVVEGEVARPNGLCFSPDESTLYLVESGTT
                     PRNVYAYEVRGGRKLSNKRKFIDVGPGTPDGMRCDVDGNLWMGWGMGDAALDGVNIYN
                     PDGKLIGRIDLPERCANLCFGGTYRNRLFMAAAKSVYALYVNTQGAAGG"
     gene            1365..1688
                     /locus_tag="VE030_01200"
     CDS             1365..1688
                     /locus_tag="VE030_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HZF22063.1"
                     /translation="MLLDVRTYTCRPGTIRKHLALYEKMGKGPQTRHLGQPFAYLVTE
                     TGNVNQYLHIWAYEDAADRTRRRAAMMADPEWIAFMEESAKLGALEHQENCLMTPVPF
                     FPLKR"
     gene            2074..3714
                     /locus_tag="VE030_01205"
     CDS             2074..3714
                     /locus_tag="VE030_01205"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HZF22064.1"
                     /translation="MRLPSCLLLLIMAFAAALVPAMADPPDQQNAIPSFAELQDAGAV
                     VGEIRIDALNIFDLDDPKENNVLFRLANKLHIRTRPGVIRRMLLFKSGEPVSVRLIEE
                     TERLLRSNRYLYDVSIRPVAYHDGVVDVEVKTRDTWTLDPGLRYGRAGGANSGGIGLK
                     DDNLLGTGTSLGVSRTSDVDRNSTEFQISQGHAFGGWTSIAYSYATLDDGERQSFNLA
                     RPFYALDTRWAAGLSLSKDNRIDSVYNSGTIASQYRHRKDSAETYGGWSEGLIDGWTR
                     RYSIGLTYQDDTYEMEPNLIAPAELPSDQTLVAPFFRYEVIQDAYQKFKNRDQIERPE
                     YFAMGFQSSLQLGRSLTALGSSREVWLYSGRVSNGFELLWKHTLLVSGSLSGQYGNDR
                     GEHQLYSGSARYYAPHGTRALFFAGVSGDIVRNPDISDLLQLGGDNGLRGYPLRYQSG
                     DRRALLTLEERVYTDWYPFHLFRVGGAVFFDVGRAWGGDNQNTANPGWLSDAGFGLRI
                     LNARTAFGNVLHADVAFPLNADPGIKSVQFLFKTKVSF"
     gene            3905..4261
                     /locus_tag="VE030_01210"
     CDS             3905..4261
                     /locus_tag="VE030_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22065.1"
                     /translation="MISDNWAVLFTQFRTRTEVRGVLSRTLTNMMDPAKDPAKFPSEL
                     SGLATGNDREVMESYLRVKIFCDGKKGKDRGSVVGGIAGDVRDLFAKALERMKELPPE
                     AITLARIATVRHVRRA"
     gene            4285..4608
                     /locus_tag="VE030_01215"
     CDS             4285..4608
                     /locus_tag="VE030_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22066.1"
                     /translation="MSTSDSLMYQEVLSKAFAGIKTILRRRQLDPLRYIVDIDSGQPV
                     FALRDTHLLRIAYNRHEVLSMEVAIPHQWLVGQAGEDHENFLRAVDDMVLELKGKVQA
                     ARRLL"
     gene            4738..5016
                     /locus_tag="VE030_01220"
     CDS             4738..5016
                     /locus_tag="VE030_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22067.1"
                     /translation="MTEYPTQTELLSYLTILAGATGDPTEVPFRVDIIPAHGKPPMYS
                     VMIKSPHKDRLRRQIGSILSRPFALGATSFMLTGNEAVSLIKHGHPAI"
     gene            complement(5009..5683)
                     /locus_tag="VE030_01225"
     CDS             complement(5009..5683)
                     /locus_tag="VE030_01225"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HZF22068.1"
                     /translation="MKVLVLCICTFLGIGFYGAHAAAAELANDEQKTLYAIGVALSQS
                     LHDFSLSEAELEIVKSGLSDGVLKRSIKVDMQTYGPKIELMGKARGAAVAEKEKKAGA
                     AFLAKAAAEPGARKTASGAIVKTLKEGGGPSPSATDKVKVHYHGTLVDGTVFDSSVKR
                     GQPVTFPLGNVIKCWTEGLQQMKVGGKSRLICPASIAYGERGSPPIIRPGATLIFDVE
                     LLDIVK"
     gene            complement(5732..6337)
                     /locus_tag="VE030_01230"
     CDS             complement(5732..6337)
                     /locus_tag="VE030_01230"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZF22069.1"
                     /translation="MPAFTHFLIIANVGIFLLQLAGGGSLIQWFALWPGDGAGLASLP
                     LLTPWQLVTYSFLHGNLMHLAFNMFALWMFGAELERVWGARRLATAYFASVVTGAVTH
                     LLVAGAFGGGGGPVVGASAGVFGLLLAYALLFPNRRIMLLFPPIPMPARVFVLLYVLL
                     ELYLGVTGTQSGVAHFAHLGGLLGGWWVYRFGRSRPRYWRG"
     gene            6627..6776
                     /locus_tag="VE030_01235"
     CDS             6627..6776
                     /locus_tag="VE030_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22070.1"
                     /translation="MTDMKPAKTAVEIQPLVRYTPEETARNKKARDERRKAIRHQNAR
                     SKSLK"
     gene            6854..7462
                     /gene="gstA"
                     /locus_tag="VE030_01240"
     CDS             6854..7462
                     /gene="gstA"
                     /locus_tag="VE030_01240"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HZF22071.1"
                     /translation="MKLYYSPGACSMAPHIVAREAGHKVDLEKVDIPAKKTAGGEDYW
                     TINPKGYVPALKMDDGQVLTEVGVIIQYLADRKPESGLTAKMGTMERYRQMEAVNFAA
                     TEIHKQLGALFNPKMTPEMKEVQLGTVERRFNALEKLLAGKQYITGDKYTVADAYLFT
                     VLNWTKGLNIDLSKWPNIKNLVARVAARPAVQETMKAEGLIK"
     gene            complement(7545..8174)
                     /locus_tag="VE030_01245"
     CDS             complement(7545..8174)
                     /locus_tag="VE030_01245"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZF22072.1"
                     /translation="MKLNREELARTSALTLDHYNERAEAFREGTRDHDVSQNIAALLQ
                     AIEGRPPFTILDFGCGPGRDLKVFAALNHVAIGLEGAARFATMARNHSGCEVWEQDFL
                     KLDLPEGRFDGVFANAALFHVPSQALPRVLRELRTSLKPGGVLFSSNPRGQDQEGWNN
                     GRYGVYHDLETWRRHLLAEGFVELAHYYRPKGLPFEQQPWLASLWRRPL"
     gene            complement(8536..9411)
                     /locus_tag="VE030_01250"
     CDS             complement(8536..9411)
                     /locus_tag="VE030_01250"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HZF22073.1"
                     /translation="MAPTFRKLHPHFVAEASPIDLRQAHDGDTLDAIRAGMDEYAVLI
                     FRDQPFSDTEQLAFAQRFDGTLHSNTGAAVLGKNRFGNEALTDISNLDEDGAILKSDD
                     RRRMYALGNRLWHTDASFQDPPGRYSMLSARVVPPAGAGTEFADMRTAYDALDAGTKT
                     QLEGLRAHHSIAYSRQILGFEFSSQEQERLKGAVHPLVHTNPRTQRRSLYLASHASRI
                     IDWPLPEGRLLLRDLIEHATQARFVYRHVWRPGDLVIWDNRATMHRGRPFDDAKHRRE
                     LRRVTTLDLGSPAGT"
     gene            complement(9581..10534)
                     /locus_tag="VE030_01255"
     CDS             complement(9581..10534)
                     /locus_tag="VE030_01255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HZF22074.1"
                     /translation="MPSLRAALLIFGYVIVSPASIAAEVSTLVIGTATPGGGFTVYGE
                     ALAETLRETDPTLDVRPRPTAGSKENIPLLESGQLDLGLVEGTAAYEALTGIGRSPAP
                     LSVVAAMYPSPGMFAVRADSPYRIVADLKGQRVVFGAAGSGFVLFARYVLDGLGLDMH
                     KDFDAVLLEKVKDAPPMVLSGEAAALWGGGIGWPGFEAIAHGPKGARFIGLEPDDISR
                     IQRKYPFLKTMSVAEGTYPGLDRAIATVGSWGLILARPDLPDDLAYRFARALHRAQPA
                     LEQRLAQARETTPANTLTASPRTEFLHAGVARYLRETGLMQ"
     gene            complement(10785..11459)
                     /locus_tag="VE030_01260"
     CDS             complement(10785..11459)
                     /locus_tag="VE030_01260"
                     /inference="COORDINATES: protein motif:HMM:NF025731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superinfection immunity protein"
                     /protein_id="HZF22075.1"
                     /translation="MFDALSQRFPGYEDWTIALLPVLLIAYFTPLLIAVLRKHRFITP
                     IGLINFLLGWTVVGWLAAIVWAVNKDVREALEDPEPVAGPLYSMNEPSWNELAAESQA
                     AAQESAKKCSFCAEEIRAEAIVCRYCGRDVAPASANGQLGAVASAVSIEQNFEELKSL
                     LQDRDEIAAQELAEGKFQAATNYVDSREKVSKEPVVGAGKDAVSAEMDWENSLWKKMG
                     VEKNRA"
     gene            11629..12174
                     /locus_tag="VE030_01265"
     CDS             11629..12174
                     /locus_tag="VE030_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22076.1"
                     /translation="MNPISKRSLSPLACAAAAAVLWAGATAAVACGEVFRGDIVTADR
                     EVGATPSGEAATFHVLGAGWFRAAEVVKHGGNSDKTYVTLELDGEPVITTSFAALKNP
                     WMQLNTSYIVANVQTEGDTSTMTIWYSPELKFSAMAVLRINVEEDGVDSLKMRTVMNK
                     PAPHEHIAGQIGAVAALPAFK"
     gene            complement(12178..12831)
                     /locus_tag="VE030_01270"
     CDS             complement(12178..12831)
                     /locus_tag="VE030_01270"
                     /inference="COORDINATES: protein motif:HMM:NF023164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3299 domain-containing protein"
                     /protein_id="HZF22077.1"
                     /translation="MTRRRRYILILLCALLTPNASADEPRQVMWADLVPKAAAMDNPF
                     LKLTKEQLAWVSDVAAVRDRKDRGDRTLSATERENEQALSRKLAVAGIDVDGLLAARM
                     KIIERGRAVNPLLNGQNVRLPGYLLPLEFSGKQVSEFLLVPWVGACIHTPPPPPNQIV
                     HVRPEKPVEMGGMFAPVWVTGQMTTGAIKKSLSLVDGSADIDVGYSLRASAVEPYKE"
     gene            complement(12853..>14095)
                     /locus_tag="VE030_01275"
     CDS             complement(12853..>14095)
                     /locus_tag="VE030_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZF22078.1"
                     /translation="RNRKFTAVLTLLSIALAVMLLLGVERLRSESRESFAATISGTDL
                     VVGARSSPVHLLLFSVFRIGNATNNIRWDSYSAIAQRPEVAWAIPLSLGDSHRGFRVL
                     GTAREYFEHFRFGRDHKLELAQGGPFDDLHDAVLGADVAAKLGYKPGDSIVIAHGAGE
                     VSFSLHEDQPFRVVGVLARTGTPVDRTVHVTLEGLDAVHAEASEDAFDPLAAAGRAGA
                     GEHEHHAGKKTERAITAFLVGLKSRGAALSMQRLVNEYSDEPLTAILPGATLLEVWEI
                     VGAAEKTLFAVSALVVVVGLAGMLVALLTSLGERRREMAVLRSVGARPVHIFGLILGE
                     AAFLTLLGIALGIAALYLGLVAGRPWLESRLGLFIVVGWPSAYEFALMIVVAAAGVLI
                     GLIPAYRIYRYSLADGMTIRI"
BASE COUNT         2785 a         4412 c         4341 g         2557 t
ORIGIN      
        1 gaaacgggag agcggacgtc ctcatatcgc caggaagaaa gaaaccttgt caaagagacg
       61 cgagccccac gaaagtgttg ccccgatgcg agaatgtctg gcggatatta ccggatcact
      121 caggcgcccg tttcttttcg atgcgcccac gcggttgccg tgcaattcat cagccacatg
      181 ccaagtcgac agtcgttttt caagtgccgc catttcttcc ggacggcttc cctccgttcc
      241 ccgccggtgg cggccgcggg ctgcacccct gtaaactgca cgacgaccat tcagagaacc
      301 ggatcattta tgccacccga gaacgccgaa cccaaggatg cccccgatat gccctggcag
      361 ccgagccagc gttacccgga ttccgcagtc cagatcatcg accagagctt caccaggtac
      421 cgcctcaatc ttgcggcggt cgagcggatc gcccacggat ttcgctggag tgagggtccg
      481 gtgtggttcg gcgacgggcg ttacctgctg tggagcgaca tccccaacaa ccggatcatg
      541 aagtgggagg aagaaaccgg cgccgtgagc atttttcgcc ggccttccac cttcgccaac
      601 ggcaacacgc gcgaccggca ggggcggctc gtcacctgcg agcacggcgg gcggcgcgtc
      661 acgcgcaccg agtacgacgg cacgatctcg gtgatcgccg gcgccttcgg gggcaagccg
      721 ctcaattcgc cgaacgacgt gatcgtcaag tccgacggct cgatctggtt caccgacccg
      781 cccttcgggc tgctgggcta ctacgaaggc cacctcgcca aacaggaact gccgatgaac
      841 gtgtactgcg tcgatggcca gagcggcgaa atgaccgttg tcgaaggcga ggtcgcccgg
      901 cccaacggac tgtgcttttc tccggacgaa tcgacgcttt atctggtcga gtcgggcact
      961 acgccgcgca acgtgtacgc ctatgaggtg agaggcggcc ggaagctatc caacaagcgc
     1021 aagttcatcg acgtggggcc gggcacgcct gacgggatgc gctgcgacgt cgacggcaat
     1081 ctctggatgg gctgggggat gggcgacgcg gcgctggacg gcgtgaacat ctacaacccg
     1141 gatggcaaac tgatcggccg catcgacctg cccgagcgct gtgccaacct ctgcttcggc
     1201 ggcacctacc gcaaccgcct gttcatggcg gcagccaaga gcgtctacgc gctctatgta
     1261 aatacgcagg gagcggccgg aggctgaatc gatgcgcact attcccgatg acatgcaaga
     1321 tgcaagacct tacctcgccc ttttgatatt ttgtggagga agacatgctg ctcgatgtac
     1381 gtacctatac ctgccgcccc ggaaccatca gaaagcatct cgcgctctac gagaaaatgg
     1441 gcaaaggacc gcagacccgc cacctcggcc agccctttgc ctatctcgtg accgagaccg
     1501 gcaacgtcaa ccagtacctc cacatctggg cctatgagga cgccgcggac cgcacgcgcc
     1561 gccgcgccgc gatgatggcc gacccggagt ggattgcatt catggaagaa agcgccaagc
     1621 tcggcgcgct ggagcaccag gaaaactgcc tgatgacgcc ggtgccgttc tttcctctca
     1681 agcgctgagc gaaaaccatg cgcgaggacg gcttggcgag tgtgcggtcg cgcaactgcc
     1741 gtcaggctcg gtccaaatcg gtcgcgcgcc tttactgcgt cagttctaaa ccgtgcgcgt
     1801 gctggtcgtc gaagacaata ttgaatcggc taccggctta ttgcttccga tgagcgggtc
     1861 cagatcactt tagcgacgca tcgtcgtcgc gtatctgctt cttgcgcctg gatggcatgt
     1921 cttctttgcg ctgatcgcgg tccgacgcgc caggacacct cgagttggta ccgtaaccgc
     1981 gcatccatgc acatcggacc gcagccccct attccccggt cggcgcaaca ccttccggaa
     2041 gtaatgccgg ttgggattcg cggggtcgcg acgttgcgtt taccaagctg cttgctgctc
     2101 ctgatcatgg ccttcgccgc ggcgttggtc ccggcaatgg ccgatccgcc ggaccagcaa
     2161 aacgccattc cctccttcgc cgaactccag gatgccgggg ccgtggtcgg agagattcgc
     2221 atcgacgccc tcaacatttt cgatctggac gacccaaagg aaaacaatgt cctcttccgc
     2281 ctcgcgaaca agctgcacat tcgaacgcgt cctggcgtca tccggcgcat gctgctgttc
     2341 aagagcggtg agcccgtgtc ggtacgcctc atcgaagaaa cggaacggct gctgcgctcc
     2401 aaccgctacc tttacgacgt cagcatccgg ccggtcgctt atcacgacgg cgtcgtggac
     2461 gtcgaagtca agacgcgtga cacctggaca ctggatccgg ggctgcgtta cggccgtgcc
     2521 ggcggcgcca actcgggcgg cattgggctg aaggacgata acctgctggg taccggcaca
     2581 tccctcggcg tatcgcgcac ctccgatgtc gatcgaaaca gcaccgaatt ccagatttcg
     2641 cagggtcacg ccttcggcgg ctggacttcg atcgcttact cgtacgccac gctcgacgat
     2701 ggcgaacgcc agtcgttcaa tcttgccagg ccgttctatg cgctggacac gcgctgggcc
     2761 gccgggctaa gcctgtcgaa ggacaaccgc atcgattctg tctacaacag cggaacgatt
     2821 gccagccagt atcgccatcg gaaggattcg gccgagacct acggcggctg gtcggagggc
     2881 ctcatcgacg gctggacgcg ccgatactcc attggcctca cctatcagga cgacacttac
     2941 gagatggaac cgaatctgat cgcgcccgcg gagttgccgt ccgaccagac gctggtcgcg
     3001 ccgttctttc gctacgaagt catccaagac gcttaccaga aattcaagaa tcgggaccag
     3061 atcgaacgtc ccgaatactt cgccatgggc ttccagtcaa gcctgcaact gggccgctcg
     3121 ctgactgccc tcggctccag ccgcgaggta tggctctact cgggcagggt gagcaacggc
     3181 ttcgagttgc tatggaaaca taccctgctg gtgtcgggct cactttccgg tcagtacggg
     3241 aatgacaggg gcgagcacca gttgtacagc ggatctgcgc gttactacgc gccacacggc
     3301 acgcgcgcgc tgttcttcgc gggcgtttcc ggcgatatcg tccggaatcc cgacatttcc
     3361 gaccttctgc aattgggcgg ggacaacggc ttgcgcggct acccgctgcg ctaccagagt
     3421 ggagaccggc gtgcgctgct caccctcgag gagcgtgtct ataccgactg gtatccgttc
     3481 cacctgtttc gtgtgggtgg cgcggtgttc ttcgacgtgg gccgcgcctg gggtggcgac
     3541 aaccagaata ccgccaaccc gggctggctc agcgatgccg ggttcggcct gcgcatcctc
     3601 aacgcccgca ccgcgttcgg caacgtgttg cacgccgatg tcgcattccc gctgaatgcc
     3661 gatcccggca tcaagtccgt tcagtttctg ttcaagacca aggtcagttt ctgagccgcc
     3721 atcgagaccg gccatgtcaa tcagggaagc tctgaccggg ttccgcaatc cgcccgtgcc
     3781 ctgccgggtc cagaaatata cccggctgca ctcgccaata agggtcatac tgtacgagcc
     3841 gcgcgccgcg tctgtctggc ccgccatccc tatgaatgtc gtttcaatcc taccggggcg
     3901 aacaatgatc agtgacaatt gggccgtgct attcacgcag ttcaggacgc gcaccgaagt
     3961 gcgaggcgtc ctgtcccgca cactcaccaa catgatggac cccgccaagg atccagcgaa
     4021 gtttccgtcg gagctcagcg ggcttgccac cggcaacgac cgtgaagtga tggaatctta
     4081 tcttcgcgtg aaaatcttct gcgacggcaa gaagggaaag gaccggggtt cggttgtagg
     4141 tggcattgcg ggcgatgtcc gcgacctgtt cgccaaagcg ctggagagaa tgaaggaact
     4201 gcctcccgag gccattacgc ttgcccggat tgccactgtg cgccacgtgc ggcgcgcctg
     4261 agcaccggtg atgggctgcg aacgatgagc acttccgatt cgctcatgta tcaggaggtg
     4321 ctgtcgaagg cctttgcggg tatcaagaca atcctccggc gccggcaact ggacccgctg
     4381 cgttacatcg tggacatcga ttccggacag cccgtcttcg cacttcggga cacgcacctt
     4441 cttcggatcg catacaaccg ccacgaagtt ctgtcgatgg aagtcgccat tcctcaccag
     4501 tggctggtgg gacaagccgg ggaagaccac gaaaattttc tgcgcgcagt tgacgacatg
     4561 gtcctggaac tgaaaggcaa ggttcaggca gcaagacgcc ttctttaagg atcatttccg
     4621 gaacacagcc gatacggcgg ccgttggtct ggtgcagtcc gtcgcggaac tgacaccgct
     4681 cacgatcgcc ttcgccgggt gtccggctgc tgtcggaacc cgaccatacc tcgaaccatg
     4741 accgaatacc ccacacaaac cgaacttcta agctatctca ccatccttgc gggcgctacc
     4801 ggcgatccga ccgaggtgcc gtttcgcgtg gatatcattc ccgcccacgg caagccgccg
     4861 atgtattccg tgatgatcaa gtcgccgcac aaggaccgcc tgcgccggca gatagggagc
     4921 atcctgtccc gtccgttcgc gctgggcgcc acctccttca tgctcaccgg gaacgaagcg
     4981 gtctcgctga tcaagcacgg gcaccctgct atttgacgat atcgagcagt tcgacgtcga
     5041 atatcaaagt ggcgccgggc ctgatgatgg gcggtgatcc tcgttcccca taggcgatgc
     5101 tggccgggca tatcaggcgg ctcttgccgc ccaccttcat ctgctgcaag ccttcagtcc
     5161 agcacttgat cacgttgcct agcgggaagg tgaccggctg gccgcgcttg acggaactgt
     5221 cgaacaccgt cccgtccacc agggtgccgt gatagtggac cttcaccttg tcggtggcgc
     5281 tcggcgacgg gccgccaccc tccttgaggg tcttgacgat cgctcccgac gcggtttttc
     5341 tagcccccgg ttccgcggcg gcctttgcca agaacgcggc tccagccttc ttttcttttt
     5401 cggcgacagc ggcgccacga gcttttccca tcagctcgat cttcggtccg taggtctgca
     5461 tatccacttt gatggatcgc ttcagaacgc catcggagag gccggacttc acgatctcga
     5521 gttcggcctc gctcagcgag aaatcatgca gcgactggct gagcgcgaca ccgattgcgt
     5581 agagggtttt ctgctcgtcg ttcgcaagtt ccgcagcagc agcgtgcgcg ccgtagaagc
     5641 cgatgccgag aaatgtgcaa atgcagagca ccaggacttt catgaggaat ccttttcgtg
     5701 aaaagttgcg atagtacccc agcactggct ctcagcctcg ccagtaacgc ggtcggctgc
     5761 gaccgaagcg atagacccac caaccgccca gcaggccgcc caggtgcgca aaatgggcca
     5821 cgccggattg cgttccagtg acgcccagat acagttccag gagtacgtag agcagcacga
     5881 acacgcgcgc aggcatcggg atcggcggga aaagcagcat gatccttcga ttcggaaaaa
     5941 gcagcgcgta cgccagcagc aggccaaaca cgccggccga agcccccacg acaggcccac
     6001 ccccgccgcc gaaggccccg gccaccagca ggtgcgtcac agcacctgtc acgacgctcg
     6061 caaaatatgc cgtggccaga cggcgcgcgc cccagacgcg ttcgagctcg gctccgaaca
     6121 tccacagcgc gaacatgttg aacgccaggt gcatcaggtt gccgtgcaga aaactgtagg
     6181 tcaccaactg ccatggagta agcagcggca gactcgcgag gccggcaccg tcacccggcc
     6241 agagggcgaa ccattgaatg aggctgccgc cccccgccag ttgcagcagg aaaatgccga
     6301 cgttggcgat gatgaggaaa tgggtgaatg cgggcatgcg ggcctctacg ggttgggttg
     6361 actggcttca gatgtagcca ttgtccgacg gcgagaaggt ggggccgttc aacttgtcgg
     6421 gacgagtacc accgagaatg gagtctggcc cccgtctttt caagatggaa ggaggggcag
     6481 ggggcccgtg acctgaagag ataggccgag aaatgcgcgc ggcgaaaagg gaagagccaa
     6541 gggccgacgc gccaccccgt gaaggttacc gcacgcactt tctcgtctca tccctgtata
     6601 gtggcggccc catcaggaga gccgacatga cagacatgaa accagcaaag actgccgtag
     6661 agatccagcc gcttgttcgt tacacgcccg aagagactgc ccgcaacaaa aaggcccgcg
     6721 acgagcggcg caaggcgatc cggcaccaga atgcccgcag caagtcgctg aagtagccgc
     6781 ccacaacgtg ccggcacatc ggtaccattg attgcgggct cgatcccgtc gcccatcgga
     6841 aaggaatgca ctcatgaagc tttactattc acccggagcc tgttcaatgg ccccgcatat
     6901 cgttgcccgc gaagcgggcc acaaggtcga tctggaaaag gtcgacatcc ctgccaagaa
     6961 gaccgccggc ggcgaagact actggacgat caatccgaag ggctatgtgc ctgcactaaa
     7021 aatggatgac gggcaggtac tcaccgaggt cggcgtcatc attcagtacc tcgccgaccg
     7081 caagcccgag tccggcctga cggcgaagat gggcaccatg gaacgctatc gccagatgga
     7141 agcggtcaac ttcgccgcca ccgagatcca caaacagctc ggcgcgcttt tcaatccgaa
     7201 gatgacgccg gaaatgaaag aggtccagct cggcaccgtt gaacggcgtt tcaatgcgct
     7261 ggaaaaattg ctcgccggca agcagtacat caccggcgac aaatacaccg tggccgatgc
     7321 gtatcttttc acggtgctca actggaccaa ggggctcaat atcgatttga gcaagtggcc
     7381 gaacatcaag aatttggtgg cccgcgtcgc cgccaggccg gcggtgcagg aaacgatgaa
     7441 ggcggaaggg ctgatcaaat aagcagaaga gggcggaccc ggcgcgcgcc cgagtgttga
     7501 atgcgtatgc caccggcggg ctcttcggcc atcgggcaca tcgattacaa aggccggcgc
     7561 cagaggctcg ccagccaagg ctgctgttcg aagggcaggc ccttcgggcg gtaatagtgg
     7621 gcaagttcga cgaacccctc agccagcaga tggcggcgcc atgtttcgag atcgtgatac
     7681 acgccatagc gcccattgtt ccagccttcc tggtcctggc cgcggggatt ggaactgaac
     7741 agcaccccac ccggtttcag gctcgtgcgc agttcccgca gcaccctcgg taacgcctgg
     7801 ctgggtacat gaaacagggc ggcgttggcg aacacgccat caaaccggcc ctccggcaga
     7861 tcgagcttga ggaaatcctg ctcccacacc tcgcagccgc tgtggttgcg tgccatcgtc
     7921 gcaaagcgtg cggcgccttc caggccgatc gcaacatgat ttagtgccgc gaataccttg
     7981 aggtcacgcc ccggcccgca gccgaaatcg aggatagtga aaggcggcct gccctcgatg
     8041 gcttgcagca gcgcggcaat gttctggctg acgtcgtgat cgcgagtgcc ttcccggaac
     8101 gcttcggcgc gctcgttgta gtggtcgagc gtgagggcag atgttcttgc cagttcttcg
     8161 cgattcagtt tcatgttcga ccagtggatg atttgccgga gacgcgcggg gctgctagaa
     8221 aagacactgg ccccgcgctt tattcgtttg ctacactata gcctggcaac tttgaaagga
     8281 ggtgatcccg tgagcgacct acaaggcaat gccggatggc aaaccggatc aacccgcagt
     8341 ttcaggagtt tggcccggta gttcaggccg gattgcctaa gcagcaaaag cgccccgatc
     8401 gtgaaagcga acggggcgtt tcattttccg ggacgcgaaa aggcgtccgg catatgcctg
     8461 gagaagaatg ctctcaggtg gcggaaaacg tgcggctgcc gcatcttcac ggcaggccgg
     8521 gcgcgggcga agcggtcagg tgccggcggg tgacccgagg tccagcgtgg taacgcgacg
     8581 cagttcccgg cgatgctttg cgtcgtcgaa gggccgcccg cggtgcatgg tggcgcggtt
     8641 atcccaaatc accaggtcgc cgggacgcca gacgtggcgg tatacgaatc gtgcctgggt
     8701 cgcatgttcg atgagatcac gcaggaggag ccgcccctcc ggcagcggcc agtcgatgat
     8761 ccgcgaggcg tgcgacgcga ggtagagcga ccggcgttgc gtgcgcgggt tggtatgcac
     8821 cagcggatgc acggcgcctt tgagccgttc ctgttcctgg cttgagaatt cgaagccgag
     8881 tatctgtcgc gagtaggcga tcgaatggtg tgcgcgcaac ccctcgagtt gcgttttggt
     8941 gccggcgtcg agggcgtcat acgccgtgcg catgtcggca aactcggtgc cggcgcccgc
     9001 agggggaacg acccgcgccg aaagcattga gtagcggccc ggcgggtcct ggaaggacgc
     9061 atcggtatgc catagccggt tgccgagcgc gtacatgcgg cggcggtcgt cggatttgag
     9121 gatcgcgccg tcctcgtcca gattagagat gtcggtcagc gcctcgttgc caaagcggtt
     9181 cttgcccagc acggcggccc cggtgttgga atgcagggtg ccgtcgaagc gctgcgcgaa
     9241 cgcgagctgt tccgtgtcgc tgaacggctg gtcgcggaat atcagaacgg cgtactcgtc
     9301 cattccggcg cggatcgcat cgagcgtgtc gccatcgtgg gcctggcgca gatcgatcgg
     9361 gctcgcctcg gcgacgaaat gcggatgaag tttgcggaac gtcggggcca tgaccagtct
     9421 cccggaagag cgatgtctcc cgcatcgcgc ataggataat ccccctttgc ctgcattgtg
     9481 ggcctcgacg cattgatatg gtctcggctg aggccggttg cgtggcaatg ttgctctgca
     9541 ggttggaacc ggctctgggc taggagcgca agtccgggga ttactgcatc agcccggtct
     9601 ctctcaagta gcgcgcaaca cccgcgtgca ggaactcagt gcgaggagag gcggtgaggg
     9661 tgttggccgg ggtagtttcc cgcgcctgcg caagccgctg ctccagcgcc ggctgggcac
     9721 ggtgcagggc gcgcgcaaag cggtaggcga ggtcatcggg caggtccgga cgcgcgagaa
     9781 tcaggcccca ggagcccacg gtggcaatgg cgcgatcgag cccgggataa gtaccctctg
     9841 cgaccgacat ggttttcaga aacgggtact tcctctggat tcgggaaatg tcgtccggct
     9901 ccaggccgat gaagcgcgct cctttcgggc cgtgagcgat cgcttcgaaa cccggccagc
     9961 cgatgccgcc gccccacaac gcggcggctt ccccgctcag caccatcggc ggagcgtctt
    10021 tcactttttc cagcagcacg gcatcgaagt ccttgtgcat gtccagaccg aggccgtcga
    10081 ggacatagcg cgcgaacagc acgaagccgg aacccgccgc gccgaaaacg acgcgctgac
    10141 ccttcagatc cgcgacgatc ctgtaaggcg agtcggcgcg cacggcgaac atgccggggc
    10201 tcgggtacat ggccgcaacc accgacaacg gcgcgggcga acgcccgatt cccgtcagtg
    10261 cttcgtacgc ggcggttcct tcgaccagcc ccaggtcgag ttggcctgat tcgagcagag
    10321 ggatgttctc cttgctgccc gcggtcggcc ggggcctgac gtcgagggtc ggatcggtct
    10381 cacgcagcgt ctctgcgagc gcttcgccgt aaacggtgaa acccccgccc ggtgtcgccg
    10441 tgccgatcac cagagtggag acttccgcag cgatcgaagc ggggctcacg atcacgtagc
    10501 cgaaaatgag cagtgcagcg cggagggaag gcatgcgaat ttgatccgta aagccgatgc
    10561 tgctatggaa tagccggccc aaggccggcg atacaagcgt tgaccgggaa gggcaaggat
    10621 gaaggcgaaa ggatgaggga tgaaggaact gccagggctg ttactgacaa ttgatgattg
    10681 gcaaccaagg gcgcgctgtc tccgtgttct ccgtggccag agggttattg cttttctttg
    10741 cgtacctttg cgttgacaag gtttggaatt ctgtggcgcg gggatcacgc gcggtttttc
    10801 tccaccccca tctttttcca cagcgaattt tcccagtcca tttccgccga aaccgcgtcc
    10861 ttgcctgccc ccacaacggg ttccttggag actttttccc gcgagtccac gtagttcgta
    10921 gctgcctgga acttgccctc ggcgagttcc tgcgcggcga tttcgtcgcg gtcctgcagc
    10981 agagatttca gctcctcgaa gttttgctcg atcgacactg cagacgccac cgcgcctagt
    11041 tgcccgttgg ccgacgccgg cgcgacgtca cgcccgcagt agcggcagac gatcgcctcc
    11101 gccctgatct cttcggcgca gaacgagcac ttcttcgccg attcctgtgc tgcggcctga
    11161 ctttccgcgg ccagctcgtt ccagctcggc tcgttcatcg aataaagcgg gcctgccacc
    11221 ggctccggat cctcgagggc ttcccgcaca tccttgttga ccgcccaaac gatggcagcc
    11281 agccagccga caactgtcca gccgagcaaa aagttgatca gaccgatcgg tgttataaaa
    11341 cggtgcttgc ggaggacggc gatgagcagc ggagtgaagt aggcaataag gagcaccggc
    11401 aggagcgcga tggtccagtc ttcgtatcct gggaagcgtt ggctcagcgc atcaaacatg
    11461 aagaccgata tcgttgatct ccggccattg tacgacgttg atcggcaata ggaaacaggg
    11521 gcgggaatca gccggccccg gggggcggct gcaagagcgt tgataaggtt tggacctaag
    11581 gccttcggca cggtgctaga ctgtccggaa cgagagaggc gaatctgggt gaacccgatt
    11641 tcaaaacgtt ctttgtcccc gctggcatgc gccgctgccg ctgcagtgct gtgggctggt
    11701 gcaacggccg cggttgcgtg cggagaggtt ttcaggggcg acatcgtcac cgccgatcgc
    11761 gaagtgggcg ccacgccgtc cggtgaggcg gcaacatttc acgtgcttgg cgccggctgg
    11821 tttcgagcgg ccgaagtcgt gaagcacgga ggcaacagcg acaagaccta tgtcacgctc
    11881 gaactcgacg gagagccggt gatcaccacg tcgttcgcgg ccctcaagaa tccgtggatg
    11941 caactgaaca cctcgtacat cgtggccaat gtccagacgg agggcgacac cagcacgatg
    12001 acgatctggt attcgccgga actgaaattc agcgccatgg cggtgttgcg catcaatgtg
    12061 gaagaggacg gcgtcgacag cctgaaaatg cgcaccgtca tgaacaaacc cgcaccgcac
    12121 gaacacattg ccggacaaat cggcgcggtg gccgcgttgc cggcattcaa gtaaatccta
    12181 ttccttgtaa ggctccacgg cgctcgcccg caacgaataa cccacgtcga tgtcggcgct
    12241 cccgtcgacc agcgagaggc tcttcttgat cgcgccggtg gtcatctgcc ccgtaaccca
    12301 taccggcgcg aacatcccgc ccatttcaac cggcttttcc ggtctgacgt gcacgatctg
    12361 gttcggcggc ggcggcggcg tatggatgca ggcgccgacc cagggcacca ggaggaattc
    12421 gctgacctgt ttgccggaaa actccagcgg caacaaatag ccgggcaggc gcacgttctg
    12481 accattgagc aacggattta ccgctcggcc gcgctcgatg attttcatac gcgccgccag
    12541 caggccgtcc acgtcgatgc ccgccactgc gagcttgcgc gaaagtgcct gctcgttctc
    12601 gcgttccgtc gcagagagcg tcctgtcgcc gcgatccttg cgatcgcgca ctgccgcgac
    12661 gtcgctgacc caggcgagtt gttccttcgt gagcttgaga aagggattgt ccattgccgc
    12721 ggccttcggc accaggtccg cccacatcac ctggcggggt tcgtcggccg aggcgttcgg
    12781 cgtcagcagc gcgcagagca ggatcaggat gtaacgccgg cgacgagtca tcgtggcagc
    12841 actttaaatg tgtcagatac gtatggtcat gccgtcggcg agcgaatagc ggtaaatgcg
    12901 atacgcgggg atcaggccga tgagcacacc ggctgcggcc accacgatca tcaacgcgaa
    12961 ctcgtaagcc gaaggccagc ctaccacgat gaacagcccc aggcgggact cgagccacgg
    13021 ccggccggcc accagcccca ggtaaagggc cgcgattccg agggcaattc ccagcagcgt
    13081 cagaaacgcc gcctcgccca ggatcaggcc gaaaatgtgc accgggcgcg cgccgaccga
    13141 tcgcagcacc gccatctcgc ggcggcgttc gcccaggctg gtgagcaggg cgaccagcat
    13201 gcctgcgagt ccaaccacca ccaccagcgc cgataccgcg aacagcgtct tctccgccgc
    13261 tcccacgatt tcccagactt ccagcagcgt ggcgcccggc aggattgcag ttaacggttc
    13321 gtcggaatac tcattgacca gcctctgcat cgacagcgcc gcaccgcgag acttgagtcc
    13381 caccaggaag gcagtgatcg cgcgctcggt tttctttccg gcgtgatgtt cgtgctcacc
    13441 tgcaccggca cgccctgcgg ccgcgagcgg gtcgaacgcg tcttcagacg cttccgcatg
    13501 gacggcatcc aggccctcca gcgtgacgtg caccgttcga tccaccggcg taccggtccg
    13561 cgccagtacc ccgaccaccc ggaacggctg atcctcgtgc aacgaaaagc ttacctcgcc
    13621 ggcgccgtgg gcgatgacga tcgagtcgcc gggcttatac ccgagtttcg cagcgacatc
    13681 cgcgcccagc accgcgtcgt gtaggtcgtc gaaaggaccg ccttgtgcaa gttcgagctt
    13741 atgatcgcgc ccgaagcgga agtgctcgaa gtattcgcgc gcggtgccga gcacgcggaa
    13801 accgcgatgc gaatcgccca gcgacagggg gatggcccaa gccacttccg ggcgctgcgc
    13861 gatggcgctg tagctgtccc agcgaatgtt gttggtggcg ttgccgatcc ggaacacgga
    13921 aaacagcagc aggtgcacgg ggctgctgcg tgcacccacc accaggtcgg tgccagagat
    13981 cgtggcggcg aaactctccc gcgactcgct gcgaagccgt tcgacgccga gcaacagcat
    14041 taccgccaga gcgatcgaca gcagcgtcag caccgcggtg aacttgcggt tgcgc
//
LOCUS       DASQOP010000035         3712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1422182, whole genome shotgun sequence.
ACCESSION   DASQOP010000035 DASQOP010000000
VERSION     DASQOP010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422182"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..760)
                     /locus_tag="VE030_01280"
     CDS             complement(2..760)
                     /locus_tag="VE030_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZF22079.1"
                     /translation="MSSSDPLIHLADVKKVFYTDEVETHALSGIHLDIQKGEYVSIAG
                     PSGCGKSTLLSILGLLDTPSDGAYVLNEKPVENLSLSERARIRNREVGFIFQSFNLIG
                     DLTVYENVELPLTYRGLNSAERKQRVTEALESVGMGHRAKHLPSQLSGGQQQRVAVAR
                     AVGGQPSILLADEPTGNLDSTNGEAVMELLRDLHRGGATICMVTHDARYAQHADRIVH
                     LFDGLIVEEEAGTNRRAVVDAKRGGKDDGFGAIG"
     gene            complement(858..2114)
                     /locus_tag="VE030_01285"
     CDS             complement(858..2114)
                     /locus_tag="VE030_01285"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HZF22080.1"
                     /translation="MDIKRTGKSKLKKRLRTAGLIGIGVVAIGGITFGLARLKPAAPT
                     LERATAVIDTVKRGPMLREVRGNGTLVPQLVRYIPAPADGRVEKIPVQAGVEVGNGTV
                     IVELSNPQMEQEAMDAEFQVKAAQADAESLRTRLESESMTQQSAIAGINAEYSQAKLQ
                     LDSDEVLGKQGLVPDLLLKISRMHVQELGNRLRVEQQRLAISGKSTKAQINAQESRVQ
                     QLRALAKLKKDQVEALKVRAGTSGVLQQVAVQVGQQVTPGLNIARVADPASLKAELKI
                     AETQIKDVKLGQPVAVDTRNGIIQGEVQRIDPAAREGTFIVDVALTGPLPASARPDLS
                     VDGTIELERLVNVLYVGRPAFGQGQQTVGLFRLSADGQEAVRTQVTLGRSSVSTVEII
                     NGLKEGDQVIISDTSALDNYERIRVR"
     gene            2594..3238
                     /locus_tag="VE030_01290"
     CDS             2594..3238
                     /locus_tag="VE030_01290"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HZF22081.1"
                     /translation="MRVGILGSGLMGGKLGTIFARARHDVVFSYARSMEKLKKLARDA
                     KGKARAGTPLEAAHDSDALLLAVHWSRIDDVLKQSGNLSGKVIVTCSLPMNDDNTELV
                     MAHTSSGAEELAKLVPKARVVSAFNTIPSEVLFGVYEARRKARRPSLVYCGDDKGSKA
                     VAAELIRDVGFDPLDAGPLRVARYTEPFALLVAELAYEGKGGPELAYRFKWFKK"
     gene            3278..3673
                     /locus_tag="VE030_01295"
     CDS             3278..3673
                     /locus_tag="VE030_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZF22082.1"
                     /translation="MDIKRNGSQPSGKGPDEYFTGTVRIDPLVQAPAPARVLAVSVTF
                     EPGARTAWHKHPLGQTLIVTSGCGWVQSEGRAKEEIRPGDVVWCPPGEKHWHGATSST
                     AMTHIAILEHLNGKTVDWMEKVSDEQYRA"
BASE COUNT          766 a         1152 c         1041 g          753 t
ORIGIN      
        1 atcatccgat tgctccgaag ccgtcatctt tgcctccacg ttttgcgtct accactgcgc
       61 gccggttagt gccggcttct tcctcgacga tgaggccgtc aaacaagtgc acgatgcgat
      121 cggcatgctg cgcgtagcgg gcgtcgtgtg tgaccatgca gatggtggcg ccgccgcgat
      181 gcaggtcgcg cagcagttcc atcactgctt cgccgttggt ggaatccagg ttgccggtgg
      241 gctcgtcagc cagcaggatg ctcggctggc cgccgactgc ccgcgccacc gccacccgct
      301 gttgctggcc gcccgaaagc tggctgggca ggtgcttggc ccgatggccc atgccgacgc
      361 tctccagcgc ctcagtcacg cgctgcttgc gctctgccga gttcagaccg cgataggtca
      421 gcggcagctc cacgttttca tagaccgtca ggtccccgat cagattgaag ctttggaaga
      481 tgaaacccac ctcgcgattg cggatgcgcg cgcgttccga caacgaaaga ttctccaccg
      541 gcttttcgtt cagcacataa gcgccatcgc tcggcgtatc aagcaacccg aggatcgaca
      601 gcagcgtcga cttgccgcag cccgagggac cggcgatgga aacgtattcg cctttctgga
      661 tgtccaggtg gatgccggag agcgcgtgtg tctccacttc gtcggtgtag aacactttct
      721 tgacatcggc caggtgaatc aggggatcgg atgaactcat ttgggtctcc ttggaattgt
      781 ttcgttcgct acccgtctca ggtctttctc atcttgcggc caagccgcaa aacccgaacc
      841 tatcaaaccg ctcgtcatca gcgcacgcgg atgcgctcgt agttgtccag cgccgaggtg
      901 tcggaaatga tcacctggtc gccttctttt agcccgttga ttatttctac agtgctgacc
      961 gagctgcgcc ccagcgtcac ctgcgtccgc accgcttcct gtccatccgc gctcaggcga
     1021 aacaaaccga cggtttgttg gccctggcca aaggcgggcc ggcctacata taaaacatta
     1081 accaggcgct cgagttcgat ggtgccgtcc acactcaagt ccgggcgcgc actcgccggc
     1141 agtgggccgg tcaacgccac gtcaacaata aacgtgcctt cgcgcgccgc cggatcgatg
     1201 cgctgcacct cgccctggat gatgccgttg cgcgtgtcca ccgccaccgg ttgtcccagc
     1261 ttgacgtctt tgatttgggt ttcggcaatc ttcaattccg ctttcagcga cgccggatca
     1321 gccacccgcg cgatgttcaa gcccggtgtc acctgttggc ccacctgcac cgctacctgc
     1381 tgcaacacgc cgctcgttcc cgcgcgcacc ttcaaagcct ccacctgatc ttttttcagt
     1441 ttcgccagcg cccgcaactg ctgcacccgc gactcctgcg cgttgatttg cgccttcgtc
     1501 gatttgccgc tgatggccag ccgctgctgc tccacccgca aacggttgcc cagctcctgc
     1561 acatgcatgc gcgagatctt cagcaacaaa tctggaacca gcccctgctt gcccagcact
     1621 tcgtcgctat ccagctgcaa cttcgcctgg ctgtattcag cgttgatgcc cgcaatcgcc
     1681 gactgctgcg tcatcgattc gctttccagc cgcgtgcgca aactctccgc gtcagcctgc
     1741 gccgccttca cctgaaactc ggcgtccatc gcttcctgct ccatctgcgg attcgatagc
     1801 tcgacaatca ccgtaccgtt accgacctcc accccggcct gcaccggaat cttttccact
     1861 cggccgtccg ccggcgccgg gatgtagcgc actaactgcg gcaccaacgt gccgttgccc
     1921 cgcacctcgc gcagcatcgg cccgcgcttg accgtatcaa tcaccgccgt ggcgcgctcc
     1981 agtgtcggcg ccgccggctt gagcctcgcc aggccaaacg tgatgccccc aatcgccacc
     2041 actccgatcc cgatcaaacc cgccgtgcgc agccgcttct tcagtttcga ttttcctgtt
     2101 cgcttgatat ccatgaacgt gaacgtgaac taactgcgtg tggctgcgaa ctcaacctga
     2161 atccgacctg aacatctcaa cgaaaataca gttcgccgag aattccggag tcaaagccta
     2221 tgccagctgt atcacaagcg actttttcgc tagcgcccta acgcaagaag cagctatcgg
     2281 cgtaggttcg cagcaatgat tagaatgcga ggctatcgat tcagggtgag cgagcgatga
     2341 ttacaacgca gaaactgttt cgctttcggg atgagccaat cccaattttg agaaatattc
     2401 agaatatgga ggagagttcc gagcggtttc gagcaatatc gcgacaggtc gagcaagaat
     2461 aagtgccacg gacaagctct cgcgaactag aattcctcgt agcgttaatt gacaacaaaa
     2521 gagacagaaa cgtcagcaga gtgacacctg atgcccagat gatggcaggg acagacgaag
     2581 gagcgtagca cgcatgcgag ttggcatttt gggttcgggg ttgatgggcg gaaagctcgg
     2641 gacgattttc gcgcgggcca gacatgatgt cgtattcagc tatgcgcgca gcatggagaa
     2701 actcaagaag ctcgcgcgcg acgccaaggg caaggcaaga gccggcacgc cgcttgaggc
     2761 cgcgcacgac tcggacgcgc tattgctcgc agtgcactgg tcgcgaatcg acgatgtgtt
     2821 gaaacaatct ggcaacctgt cgggcaaggt gatcgtcacc tgctccctgc cgatgaatga
     2881 tgacaacacc gaactcgtca tggctcatac atcctcgggc gcggaggaac ttgccaaact
     2941 ggttccgaag gctcgggtcg tctccgcatt caacacgatt ccgagcgaag tgcttttcgg
     3001 ggtctacgaa gccagacgca aggccaggcg accgagtctg gtgtactgcg gcgacgacaa
     3061 gggcagcaaa gctgtcgccg ctgaattgat ccgcgacgtg ggttttgatc cgttggacgc
     3121 gggcccgcta cgggtcgcgc gatacaccga gccgttcgcg ctgctagtcg ccgaactcgc
     3181 gtacgaagga aaaggaggcc cggaattggc ttacaggttc aagtggttta agaaatagtg
     3241 gaccaaatca gaagacatcg tctggaggaa ttcggaaatg gacattaaga gaaatggctc
     3301 acagccctcc ggcaaaggac cggacgagta ctttaccggc acggtacgca ttgacccatt
     3361 ggtccaagcg ccggctccgg cacgcgtgct cgccgtcagt gttacgtttg agcccggcgc
     3421 tcggactgca tggcacaagc atccgctcgg gcagacccta atcgtcacgt ccgggtgtgg
     3481 ctgggtgcaa agcgagggtc gcgcgaaaga ggaaattcgg ccgggcgatg tagtgtggtg
     3541 cccgccgggt gagaagcatt ggcacggagc cacttcatcc acggcgatga cgcacatcgc
     3601 catcctggaa caccttaacg gaaaaaccgt cgactggatg gaaaaggtca gcgacgaaca
     3661 atatcgggcc tgacttgtgg aggaagaaaa tgaaaatgac caatcgacac aa
//
LOCUS       DASQOP010000036         8403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_415567, whole genome shotgun sequence.
ACCESSION   DASQOP010000036 DASQOP010000000
VERSION     DASQOP010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8403
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415567"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="VE030_01300"
     CDS             complement(<1..763)
                     /locus_tag="VE030_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017332928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZF22083.1"
                     /translation="MPDEKVLRQLNDSLNLEPLWMPFTANRQFKAAPRLLVAAKDMHY
                     LTQDGRKVLDGIAGLWAVNAGHCRQKIVDAIKKQAETIDYATGFQLHHPAAFQAAEKL
                     VSITPKGLDHVFFVNSGSEAVDTALKIALAYHRVRGEGTRRVLIGRERGYHGVGFGGI
                     SVGGIPANRKVYGSLLPRVDHLPHTHNLEKNAYSRGQPAWGAHLADELETRIIALHDA
                     SNIAAVIVEPVAGSTGVLVPPQGYLERLRQICDKHG"
     gene            987..2183
                     /locus_tag="VE030_01305"
     CDS             987..2183
                     /locus_tag="VE030_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006763850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZF22084.1"
                     /translation="MDLNYTQEEVAFRNQVHEFVRTHLPGEVSRKVLEHRRLGKDDYV
                     RWQKILFEKGWIAAGWPVEYGGTGWSPIQRYIFEEECAAAGAPRLTPFGLHMVGPVIM
                     AFGSEWQKKHYLPRILSSEDWWCQGYSEPGSGSDLASLKTRAERQGDHYIVNGQKTWN
                     THGQWADMCFCLVRTSSEGKKQEGISFLLIDMHSPGVTVRPIITLDGEHEVNEIWFEN
                     VKVPAQNLVGDDGRGWTYAKFLLSHERTGIAGVGHSKRELRYLKHIASEEQAGGRPLI
                     EDPLFRDRVAQVEIDLMALEITNLRVAAADRDGKAPGPEASILKIKGTEIQQELTELM
                     MEAVGPYALPHLPMAWGDHWIGAHVGPDYAAPLAARYFNYRKTSIYGGSNEIQRNIIA
                     QMVLGL"
     gene            2325..3512
                     /locus_tag="VE030_01310"
     CDS             2325..3512
                     /locus_tag="VE030_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZF22085.1"
                     /translation="MNFDLTEERQMLKDSVQRFLEKNCSFEQRRKMLAERMPMNAQLW
                     EGLASLGVLGVPFSPDHGGFGGGGVETMLVMEALGRHLSLEPYLATVVLAGGCVDLGG
                     ADRQKNILIPKIIDGSMLLAFAHAELNARFELGHVETRALRDGNNFVLEGEKTLVLHG
                     PIAEKFIVSARTSGNPGDAHGVTLFIVEADEEKLGGQEYPLFDGTRALDLQLGGVKVG
                     AESVIGTLDAAMPLIEAVADRAMAALCAEAVGIMDALHAATVEYLKTRQQFGQPIGRF
                     QALQHRAVDMLIHLEQARSMAVLAASRVDSPNVAERRKAIAAAKEMIGRSGRYVGQQA
                     IQLHGGMGMTDELNVSHYFKRLTAIDVLFGNAAYQRQRFATMQEDQPAAQLKAPRKSW
                     VKI"
     gene            complement(3535..4584)
                     /locus_tag="VE030_01315"
     CDS             complement(3535..4584)
                     /locus_tag="VE030_01315"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HZF22086.1"
                     /translation="MAKKLGHKEPQQISRRRFLEAAALGALASSTLGGREATGATPDG
                     WPMTEKWFPSRWGAGDQAGASNLMTSQKVLEAISLIRVGKVHSLGRIYEAGMPFFGTR
                     GWNLKIPSAPFYGPAGRNKLIANEEYLCAEIGQVGTQFDGLSHIGVETKGPHDSSGKL
                     YYNGFNGTEVHGAFGMKKLGIEHVKPLVTRAVIFDVKGLKGRNLERGEEISAQDLRDA
                     LTRQRLSESDFKVGDVALVRCGWGDQWMVVDNKKYYDGEPGVGLDASQWLVDKGACLV
                     GADNWGVEVIPNPDRDLSFPCHHLLLTMNGVFMFENLTLEGPVRDGTWIGAFFFSPVP
                     IKGATGSPGNPLFIV"
     gene            complement(4695..5126)
                     /locus_tag="VE030_01320"
     CDS             complement(4695..5126)
                     /locus_tag="VE030_01320"
                     /inference="COORDINATES: protein motif:HMM:NF025852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4437 domain-containing protein"
                     /protein_id="HZF22087.1"
                     /translation="MTKKSLLVGLSLVAAAGFGYPAAGAGVGTMSATDEMQWQDMGPG
                     SPLKFVVLWGDRSKGEYAMLLKMPAGFVAPIHAHTGDYHGVNLTGTWRHSFDGGEEKA
                     MAPGSYVFQPGMGMHGDACVGSEDCILFIHQHVKGDFIPKQ"
     gene            5480..5956
                     /locus_tag="VE030_01325"
     CDS             5480..5956
                     /locus_tag="VE030_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HZF22088.1"
                     /translation="MIVKKPKYYWEDFKVGEVHQIGEKRVDKDEIIAFAKQFDPQPFH
                     VDEGAAKASIYGGLIASGWHTVALVMRMMCDAYMLDSASLGSPGIDNLKWLKPVRPGD
                     TIRAQRTTLEVRASASRPDMGLVKTRWEVFNQNGERVMTMEGYGMFRRRDPGSPAQ"
     gene            5953..6690
                     /locus_tag="VE030_01330"
     CDS             5953..6690
                     /locus_tag="VE030_01330"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HZF22089.1"
                     /translation="MSDPFKHKVHVLFRKEDLDHERLEGKVAIVLDVLFATSTIITAL
                     ANGATEVIPTLDEAGANAEAQKHPKGSYVLAGELYAETLPGFASPTPLALLKEDIAGR
                     KLIYSTTNGTVALRQSAHAAHVYAAALLNGEAVVQRVLGSHPGQTILIVCSGSMGMVN
                     LEDLYGAGYLVDLLSNALNESSDFSDAALAARSLFRAEDAADCLLRARVGRMMIERGL
                     THEVHYAAELSSLAAVPKLVGNRLVLS"
     gene            6856..7161
                     /locus_tag="VE030_01335"
     CDS             6856..7161
                     /locus_tag="VE030_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22090.1"
                     /translation="MRYLFLAAVAIALAGPVPAPAALDCSRARSNAEKMVCSNGRLAL
                     AEERMAYAFREAIRRGADPSELMQSQRRWTSEIRDACNEVECMLKAYEDRTAELENP"
     gene            7220..8401
                     /locus_tag="VE030_01340"
     CDS             7220..8401
                     /locus_tag="VE030_01340"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZF22091.1"
                     /translation="MRRLSLTLKPAIGLLVLFMVLITNAAITYRNGVHLVEHSRKAAR
                     ALNSSRLVAELQGRLVDAETSQRGFIITGNADYLEAYSDALTSLTPTFMELYESTANE
                     PLWEQRLDALQSLVFLKFRELQTSIELRRYSGFAAAQKLTMTNKGKRTMDRIRKLIAE
                     FRSDEARAFSIQERRLQETVTTARASFWAVTLFGLVLVIYVLYTLWLNLVEVRKTEST
                     LRRQALRDPLTGLFNRRFFDAGLAQEIMRSRRSGNPASLLILDIDHFKNYNDEYGHEA
                     GDAVLRAIGQLLQTHVRGSDVACRFGGEEFVILMPDAPLESAKERGKQILEAVRGLEI
                     PHQGHLLPPVTASLGLAEFPSHARDAEGLLEAADHALYVAKRAGRDRMVISGRVEKAV
                     A"
BASE COUNT         1671 a         2570 c         2548 g         1614 t
ORIGIN      
        1 gaccgtgctt gtcgcaaatc tggcgtaaac gctcgaggta gccctggggc ggcaccagta
       61 cacccgtgga tccggcgact ggctcgacga tgaccgccgc gatgttcgat gcatcgtgca
      121 acgcgatgat gcgcgtttcg agctcgtccg cgaggtgcgc gccccacgcc ggctggccgc
      181 ggctgtacgc attcttttcc agattatgag tgtgtggcag atgatccacg cgcggcagca
      241 ggctgccgta gaccttgcgg ttggcgggta taccgccgac cgagatgccg ccgaatccga
      301 cgccgtgata gccgcgttcg cgtccgatca gtacgcgtcg ggtgccctcg ccgcgcacgc
      361 ggtgataggc cagcgcaatt ttgagcgccg tgtccacggc ttccgatccg gagttgacga
      421 agaaaacatg gtccaggccc ttgggcgtga tcgaaaccag tttttccgcc gcctggaacg
      481 cagccggatg atgcaactga aagccggtgg cgtagtcgat ggtttccgcc tgcttcttga
      541 ttgcatcgac gattttctgg cggcagtggc cggcatttac cgcccacaga ccggcgatgc
      601 cgtcgagcac tttgcggccg tcctgcgtca aatagtgcat gtccttggca gccaccagca
      661 ggcgcggcgc cgccttgaac tggcggttgg ccgtaaacgg catccacagc ggctcgagat
      721 tgagcgaatc gttcagttgc ctcaggactt tctcgtcggg catgttcatg gcgggtctcc
      781 tcggatgccc gactgcgacc gaggtcacgc agccggacac gcgcttgatt tatgggttca
      841 ttatagtgcg cggccagcgt ctgcggccac catgattgcc tggatgggag gcttccctta
      901 tcctgtggcg ctttcgtcga tacccgatac tcgaccggcc attcatcgat ccggccgggc
      961 ctttgcagcc caccaggaac ttcccgatgg atctgaacta cacccaggaa gaagtcgcgt
     1021 ttcgcaacca ggtgcatgag ttcgtccgta cccatctccc cggcgaggtg tcgcgcaagg
     1081 tccttgaaca ccgccggctc ggcaaggacg actacgtgcg ctggcagaag atcctgttcg
     1141 aaaagggctg gattgccgcc ggctggccgg tggagtacgg cggcaccggc tggtcgccga
     1201 tccagcgcta cattttcgag gaggagtgcg cggccgcggg tgcgccgcgg ctcacgcctt
     1261 ttggtttgca catggtcggg ccggtaataa tggcctttgg cagcgaatgg cagaagaagc
     1321 attacctgcc gcgcattctt tccagcgagg actggtggtg ccagggctat tccgagccgg
     1381 gatcgggttc cgacctggct tcgctcaaaa cccgcgccga gcgccagggc gaccactaca
     1441 tcgtcaacgg ccagaagacg tggaataccc acggccagtg ggcggacatg tgtttctgcc
     1501 tggtacgcac ctcgagcgaa ggcaagaaac aggagggcat ttccttcctg ctgatcgaca
     1561 tgcactcacc cggcgtgacc gttcggccga tcatcacgct cgacggcgag cacgaggtga
     1621 acgagatctg gttcgagaac gtgaaggtgc cggcgcaaaa cctggtgggc gacgatgggc
     1681 ggggctggac ctacgccaag ttcctgttgt cgcacgagcg caccggcatc gccggagtgg
     1741 ggcactcgaa gcgtgagctg cgttatctca agcacatcgc ctccgaggag caggcgggcg
     1801 gccggccgtt gatcgaggat ccgctgtttc gcgaccgcgt tgcgcaggtg gaaatagacc
     1861 tgatggcgct ggagatcacc aacctgcggg tggcggccgc cgaccgtgac ggcaaggcgc
     1921 cgggaccgga ggcctcgata ctcaaaatca aaggcaccga gatccagcag gaactcaccg
     1981 agcttatgat ggaggcggtg gggccttacg cgttgccgca cctgccgatg gcctggggcg
     2041 accactggat cggcgcgcac gtgggcccgg actacgcggc gccgctcgca gcgcgttact
     2101 tcaactaccg caagaccagc atctacggcg gctccaacga aatccagcgc aacatcatcg
     2161 ctcagatggt tttgggttta tgaacaatcc gccaaccaca gaggcacaga gacacggaga
     2221 aattcaggac agcctggtga cgagtcggaa tgacggcaga gccaagggaa actcgattca
     2281 caaagatttt ctctgcgcct ctgtgtctct gtggtgagga atgtatgaac tttgatctga
     2341 ctgaagaacg gcagatgctc aaggacagcg tgcagcgctt cctggagaag aattgcagct
     2401 tcgagcagcg ccgcaagatg ctcgccgagc gcatgcccat gaatgcgcag ctatgggaag
     2461 ggctggcgtc gctgggtgtg ctcggcgtgc cgttctcgcc ggaccacggc ggcttcggcg
     2521 gcggcggagt cgagaccatg ctggtgatgg aagcactggg ccggcatctt tcgctcgagc
     2581 cctacctcgc caccgtcgtg ctggcgggcg gctgcgtcga tctcggcggc gccgatcggc
     2641 agaagaacat cctgatcccg aagatcatcg acggctcgat gctgctggcg tttgcgcatg
     2701 ccgaactgaa cgcccgcttc gaacttggcc atgtcgagac ccgcgcgctc cgcgacggca
     2761 acaacttcgt gctggaaggc gagaagaccc tggtgctgca cggcccgata gcggaaaagt
     2821 tcattgtctc cgcgcgtacc tcgggcaacc cgggcgacgc acacggcgtg accctgttca
     2881 tcgtcgaggc cgacgaggaa aaactcggcg gccaggagta tccgctgttc gacggcaccc
     2941 gcgcgctcga cctgcaactc ggcggcgtga aagtcggcgc ggagtcggtg atcggcacgc
     3001 tggacgcggc gatgccgctg atcgaggccg tcgccgaccg tgcgatggcg gctctttgtg
     3061 ccgaagccgt cggcatcatg gatgctctgc acgccgccac ggtcgagtat ctcaagaccc
     3121 gccagcagtt cggccagccg atcgggcgct tccaggcgct gcagcaccgt gcggtggaca
     3181 tgctgatcca tctcgagcag gcgcgttcga tggcggtgct cgctgcatcg cgggtcgatt
     3241 cgccgaacgt ggcggagcgt cgcaaagcca tcgccgcggc gaaggagatg atcggccggt
     3301 ccgggcgcta tgtcggccaa caggcgatcc agttgcacgg cggcatgggc atgaccgacg
     3361 aactcaacgt cagccattac ttcaagcggc tcaccgcgat cgacgttctt ttcggcaacg
     3421 ccgcgtatca gcggcagcgc ttcgccacca tgcaggaaga ccagcccgcg gcgcaactca
     3481 aagcgccccg taaatcctgg gtcaaaatct aaattacctc accccagcca ccaactaaac
     3541 aataaaaagg ggatttcctg gcgatcctgt tgcacccttg atcgggactg gactgaagaa
     3601 aaatgcgccg atccacgtgc cgtcccgcac tgggccttcg agagtcaggt tctcgaacat
     3661 gaatacgccg ttcatcgtca gcagcaggtg gtgacacggg aagctgaggt cgcgatccgg
     3721 gttcggaatc acctccacgc cccaattgtc agccccgacc aagcaagcac ccttgtccac
     3781 cagccattgg gacgcatcaa gtcccacccc gggctcgccg tcgtagtact ttttgttgtc
     3841 gactaccatc cactgatcac cccagccgca acgcaccagc gcaacgtcgc ccaccttgaa
     3901 gtcggattcg cttagccgtt ggcgggtaag tgcgtctctg aggtcctgtg cgctaatttc
     3961 ttcccccctc tccaggttgc gtcctttcaa tccctttacg tcgaagatca ccgcgcgcgt
     4021 cacaagcggc ttgacatgct cgatgccaag cttcttcatg ccaaaggcgc catggacctc
     4081 ggtgccgttg aaaccgttgt agtaaagctt gccggagctg tcgtgcggcc ctttggtttc
     4141 tactccgatg tgagacaggc catcgaactg tgttccaact tgtccgatct ccgcgcagag
     4201 gtattcttca ttggcaatga gtttgttccg accggcaggt ccataaaagg gcgcgctggg
     4261 aatcttcagg ttccagccgc gcgttccgaa gaagggcata cccgcttcgt agattcgtcc
     4321 caaactgtgg accttcccga ccctgatcag gctgatcgcc tcaagcactt tctgtgaggt
     4381 catcagattg gaagcgccag cctgatcacc tgcgccccac cgcgacggaa accacttttc
     4441 ggtcatcggc cagccatctg gagtggcgcc cgttgcttcc ctaccaccca gcgtgctgga
     4501 agccagcgca cccaatgccg ctgcctccag gaatcggcga cgactaattt gctgcggctc
     4561 tttgtgaccc aatttttttg ccatgatttt ctcccttact gccgcccaac tacagtgtgt
     4621 gctacccgca cactgcgccc gagaagcgat cgactatttc agcaaaactg gaatcgtgaa
     4681 actaagggca ccgtctattg ctttggaatg aaatcgccct tgacgtgttg atgaatgaac
     4741 aggatacaat cctcggaccc aacgcatgcg tcgccgtgca ttcccatccc gggctgaaac
     4801 acgtaggatc ccggcgccat ggctttttcc tctccaccgt caaaggagtg gcgccaagtt
     4861 cccgtcaagt tcacgccgtg atagtcccca gtgtgggcat gaatcggcgc gacaaacccg
     4921 gcgggcatct tcagaagcat cgcgtactcg cccttactgc gatcccccca aagcactacg
     4981 aactttaacg gcgaaccggg ccccatatcc tgccattgca tttcatcggt ggcgctcatc
     5041 gttccgaccc ctgcccccgc agcggggtag ccgaatccgg cggcggcaac tagcgaaaga
     5101 ccaaccaaca gtgacttctt cgtcatgatt ttctcctcct gagtatgacg ccggccagga
     5161 cgttggccga gcggtaagac atttgttatt gcgaatcgag aaaaaatcgc tcgaagcgcg
     5221 cctctttaaa tgacgtccaa cgtgaattgg acttccgaaa tgcaggctta tttaagcact
     5281 tctgctgcca cgcttccacg tgctctatgg tgactgcaag gtaagcggaa aggcgtgaac
     5341 aacgcatgat cccaagcctg tgctgacgga tagaggcaac cctgctatgt ttgggcgggt
     5401 agcaccagag cgcggcgggt aacagtgcgg gtactgagcg gaataaagca acacagaatg
     5461 aaacgtcagt aagaacaata tgatagtgaa aaaaccaaag tactactggg aagatttcaa
     5521 ggtcggtgaa gtgcatcaga tcggcgagaa gcgcgtcgac aaggacgaga tcatcgcctt
     5581 tgcgaagcaa ttcgatccgc aaccattcca tgtcgacgaa ggagcggcga aggcctcgat
     5641 atacggcggc ctgatcgcca gcggctggca taccgttgcg ctggtgatgc gcatgatgtg
     5701 cgatgcctac atgctcgact cggccagcct ggggtcgccc ggcatcgaca atctcaaatg
     5761 gctgaagccg gtgcgtccgg gcgataccat ccgcgcgcag cgcaccacgc tcgaagtccg
     5821 cgcctccgcc agcaggcccg atatggggct ggtcaagacc cgctgggaag tattcaacca
     5881 gaacggtgag cgggtcatga ccatggaagg ctacggcatg ttccggcgcc gcgatcccgg
     5941 gagcccagcc caatgagtga tccgttcaag cacaaagtcc acgtcctctt tcgcaaggaa
     6001 gacctcgatc acgagcgcct cgaaggcaag gtggcgatcg tgctcgacgt gctgttcgcc
     6061 acttccacca tcatcaccgc gctcgccaac ggagccaccg aagtgattcc gactctggac
     6121 gaagccggtg cgaatgccga agcgcagaag catccgaagg gcagctatgt gctggccggg
     6181 gagttgtacg cggagacgct gccgggtttt gcgtcgccaa cgccgctcgc gttgctcaag
     6241 gaagacatcg ccggcaggaa gctcatctac tccaccacca acggcacggt ggcgctgcgg
     6301 cagtccgcgc acgccgcgca cgtttatgca gcagcgctgc tgaacggcga ggcggtggtc
     6361 caacgcgttc tcggaagcca tcccgggcag accatcctga tcgtctgttc cggctcgatg
     6421 ggcatggtca atctcgaaga cctttacggc gccggctatc tcgtcgatct gctgtcgaac
     6481 gcattgaacg aaagcagcga tttctcggat gcggcgcttg ccgcgcgcag cctgttccgt
     6541 gccgaagatg ccgccgactg cctgctgcgt gcccgtgtcg gccggatgat gatcgaacgc
     6601 ggtctcaccc acgaagtcca ttacgctgcg gagctcagct cgctcgcggc ggttcccaag
     6661 ctggtcggca accgcctggt gctttcctga cgcaaaggcg ttggcggata acaacggcat
     6721 agcgtggccg ggccgtccga cgcagttgtt ttcacatgaa aatttaggac cacggactcg
     6781 gtccgaatcg gccctgccgc gttaaaatgt caggtctgcc ccagatcttc ttggacgagg
     6841 taaattgagg attttatgag gtatttgttc ttggccgcag tcgcgatcgc actcgccgga
     6901 ccggttcccg cgccggcagc gctggattgc agccgggcca gatcgaatgc cgagaaaatg
     6961 gtgtgctcga acggccggct cgcgctggcg gaggagcgca tggcgtacgc atttcgagag
     7021 gccatccggc gcggcgcgga cccttccgaa ctcatgcaat cccagcgccg atggaccagc
     7081 gaaatccgcg atgcatgcaa cgaggtcgaa tgcatgttga aagcctatga agaccggact
     7141 gcggaactcg agaatccctg aagccggcgt tctccgccaa cgggggtgcg ttccccggat
     7201 acggcatcgg ggctgaatta tgcgcagatt gtcgctgact cttaaacctg cgatcgggct
     7261 tctggtgttg ttcatggtcc tgatcaccaa tgcagccatc acttaccgca acggcgtgca
     7321 tcttgtcgag cactcgcgga aagctgcgcg ggccctcaac tcctcgcggc tggtggctga
     7381 gcttcagggg cggctggtcg acgccgagac cagccagcgc ggtttcatca tcactggcaa
     7441 cgccgactac ctcgaggcct atagcgacgc gctgacctcc ctgactccga ccttcatgga
     7501 gctgtatgag agcacggcca acgagccgtt gtgggaacaa aggctggacg cgcttcagtc
     7561 gctggtattc ctcaaattcc gcgaattgca gacgtcgatc gaactgcggc ggtactcagg
     7621 gttcgctgcc gcgcagaaac ttacgatgac caacaaaggc aagcggacca tggaccggat
     7681 aaggaagctt atcgccgagt tccgcagcga cgaggcgcgc gcgttttcga tccaggagag
     7741 acgcttgcag gaaactgtta ccacggcgcg cgcaagcttc tgggccgtca cgctctttgg
     7801 cctggtactg gtgatttatg tgttgtacac gctgtggctc aatctcgtgg aggtccggaa
     7861 aacagaaagt accctgcggc gccaggcgct gcgcgatccg ctgaccgggt tgttcaaccg
     7921 gcgcttcttc gatgccggcc ttgcccagga gatcatgcgc tcccgccgca gcggcaatcc
     7981 ggcttcgctg ctgatcctcg atatcgatca cttcaagaac tataacgacg agtacgggca
     8041 cgaggccggc gacgcggtac tgcgcgccat cggccagttg ctgcagacgc atgtgcgcgg
     8101 cagcgacgtc gcctgccgtt tcggcggcga agaattcgtg atcctgatgc cggatgcgcc
     8161 gctggaatcc gcgaaggaga ggggcaagca gatcctggaa gccgttcgcg ggctggaaat
     8221 tccgcaccag ggacatttgt tgccgcccgt caccgcatcg ctgggccttg cagaatttcc
     8281 gtcccatgca cgcgacgcgg aaggccttct ggaggcggcg gaccacgcct tgtacgttgc
     8341 caagcgtgcc ggtcgcgatc ggatggtcat cagcggtcgc gtcgagaagg ccgtcgcttg
     8401 atc
//
LOCUS       DASQOP010000037         5280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_617180, whole genome shotgun sequence.
ACCESSION   DASQOP010000037 DASQOP010000000
VERSION     DASQOP010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5280
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617180"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..817
                     /locus_tag="VE030_01345"
     CDS             176..817
                     /locus_tag="VE030_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HZF22092.1"
                     /translation="MDVVIASGPLILRGLLVTLEVSVAVLLLGTLIGVMGGLSLLYGA
                     WPLRLIARVYVDTMRGIPQLVLIFAIFYGFPALGLQVSAIVAGILALGVFCGAHVSEV
                     IRGGVGSIPRGQMDAAQGIGLNFAQRLRYVIFPQALGRILPAWVNTAVEVVKASSLVS
                     LVSVVDLMMAIQQIVGRTREALLFYAVAALLYFAINYTISAIGVRLEKRYTYI"
     gene            861..1616
                     /locus_tag="VE030_01350"
     CDS             861..1616
                     /locus_tag="VE030_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HZF22093.1"
                     /translation="MIEGLVKRFGALEILCGVDLEVPKGEVVVIVGPSGSGKTTLLRC
                     INFLEEYQGGRIHLDGRLVGYRESSGGLVRLPDSIVAGMRADVGMVFQSFNLFPHMTA
                     LENVMLGLTRVRKLTPRAAEGRATSWLERVGLRDKLRAYPAQLSGGQQQRVAIARAVA
                     MEPKVMLFDEATSALDPELVGEVLGVMEDLAHSGMTMLVVTHEMRFAREVGHRIVFMD
                     KGVIVEQGAPKQIFEQPRSERLKAFLGHFTKTT"
     gene            1653..2033
                     /locus_tag="VE030_01355"
     CDS             1653..2033
                     /locus_tag="VE030_01355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HZF22094.1"
                     /translation="MPKQRISTTTGAQPVGAYSQGLCAGDFVFVSGQGPLDPKTGKVV
                     GNTIEEQTVRVLENIKAILEAGGASMADVVKVSAHLSDMSLFERYNRAYASYFPDPKP
                     TRTTVGSQLPGILVEIDAIAYLGK"
     gene            2107..3834
                     /gene="araD"
                     /locus_tag="VE030_01360"
     CDS             2107..3834
                     /gene="araD"
                     /locus_tag="VE030_01360"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinonate dehydratase"
                     /protein_id="HZF22095.1"
                     /translation="MTKRKKPEELRSQRWFGAQDLRSFGHRSRALQMGYTREDYKGKP
                     VIAIVNTWSDVNHCHSHLRERAQEVKRGVWQAGGFPLEMPAISLAEVFQKPTTMMYRN
                     FLAMETEELLRSYPVDGCVLMGGCDKTTPGLLMGAISMNLPAIYVPAGPMLRGNWQGK
                     ALGSGSDVWKYWDELRAGNITDRDWREIEEGISRSPGTCMTMGTASTMTSVAEALGFS
                     LPNASSIPAVDSNHSRMAEYSGRRIVEMVWEDLKPRDILSEKSFDNGIKVLMALGGST
                     NGLIHVIAMAGRAGIRIPLERFEEVGRHAPYLVNMRPSGKYLMEDFYYAGGLVGMLEG
                     MRKLLDTDVLTCTGKTLGENIAGAMIHNPDVIRTPENPMQAEGGLVVLRGNLAPDGAV
                     IKSTAADPKLLKHAGRAMVFDDYNDMAARFDDPNLDVDAGSVLVLRNAGPLGWPGMPE
                     WGMLPIPKKLLKQGVRDMVRISDARMSGTSYGTCVLHVAPESWVGGPLALVKTGDIIE
                     LDVPGRKLTLKVSDAELAKRRAAWKPHPPKFQRGYGKIFAEHIKQANLGCDFDFLEGT
                     TPTPEPEIH"
     gene            complement(3854..4333)
                     /locus_tag="VE030_01365"
     CDS             complement(3854..4333)
                     /locus_tag="VE030_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZF22096.1"
                     /translation="MAESKWKYHGIRIVKGSEVDTNTAQTPGMNRAAAITYARCGAEK
                     LWAGTVVIHPKAKTGAHHHGPVESVIYVVSGKARMRWGDQLEFVAEAGPGDFIHVPPY
                     VPHQEINASDSEPLSCVLCRSGQEPVVVNLDIPAVEKPEEVHWVDNIHPDPATGKRD"
     gene            complement(4348..4644)
                     /locus_tag="VE030_01370"
     CDS             complement(4348..4644)
                     /locus_tag="VE030_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22097.1"
                     /translation="MKLNLRRFLLYYLPLLANLGLIVYLTWQLNLVPYFQRQGEFQIF
                     SVLYAIGGLVVGVSGVTAFIHAVSNEKGPRNLFMLSLVNTIIPTVLLLALLQLR"
     gene            complement(4641..>5280)
                     /locus_tag="VE030_01375"
     CDS             complement(4641..>5280)
                     /locus_tag="VE030_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HZF22098.1"
                     /translation="ARDGGRPLCCGRTFLAAGLVDEARVEAQRMLDALRPYVDRGVPI
                     VGLEPSCLLTLRDEFLVMGLDGEIGPTAERALLFEEFLADEADAGRLKLDLAPLRTQT
                     ALLHGHCHQKAFDAMPAVRKVLGMIPGLKVQTVDSGCCGMAGSFGYEAEHFAVSMKMA
                     EQSLLPAVRKAGPDTIIVADGTSCRHQIHAGARRNALHVARVLQMSLEERKR"
BASE COUNT          985 a         1658 c         1701 g          936 t
ORIGIN      
        1 gccatccgca tcaacgtcaa tacgtggcgc gtgatcgagg tctatctggt ggtggggttg
       61 ctgtatctcg ccgcgtgtta cgccattgcc ggcgggctgc ggctgctgga acggcggttc
      121 gccatgattc gatgatgcct ggttcgctga tgttcggaca cacgggagcc gacgaatgga
      181 tgtcgtgatc gcgagcgggc cgttgatcct gcgcggactg ctggtcaccc tcgaggtatc
      241 ggtcgcggtg ctgctgctgg gcacgctcat cggagtcatg ggcggactat cgctgctgta
      301 cggtgcgtgg ccgctgcgcc tgatcgcacg cgtttacgtc gacaccatgc gcggcattcc
      361 gcagttggtg ctgatctttg cgatcttcta cggctttccg gctctcggtc tgcaggtctc
      421 ggcgatcgtt gccggcatac tcgcgctcgg cgttttctgc ggggcgcatg tttccgaagt
      481 gatacgcggc ggcgtgggct ccatcccgcg cggccagatg gatgccgccc agggcatcgg
      541 tttgaatttc gcgcagcgtt tgcggtacgt gattttcccg caggcgctgg gccgcatcct
      601 gccggcgtgg gtcaataccg cggtcgaggt ggtcaaggct tcctcgctgg tgtcgctggt
      661 cagcgtcgtc gacctgatga tggcgatcca gcagatcgtg ggccggacgc gcgaagccct
      721 gctgttctac gcggtggccg cattgcttta cttcgcgatc aattacacca tctctgccat
      781 cggcgtgcgt ctggaaaagc gttacaccta catctgaaga ccaacatcag atcaggatgc
      841 ccatgaccgg gaaggttgtc gtgatcgagg gactcgtcaa gcgcttcggc gcgctggaga
      901 ttctctgcgg cgtggatctg gaggttccca aaggcgaggt ggtggtcatc gtcggtccga
      961 gcggttcggg caagaccacc ctgttacgct gcatcaattt cctcgaggag taccagggcg
     1021 gccggatcca cctggacggc cggttggtag gctatcgcga gagcagtggc ggcctcgtca
     1081 ggctgccgga cagcatcgtc gccggcatgc gcgccgacgt cggcatggtg ttccagagct
     1141 tcaacctgtt cccgcacatg acggcgctgg agaacgtgat gctcgggctc acccgggtgc
     1201 gcaaactgac gcctcgcgca gccgagggac gcgcaacctc atggctggaa cgcgtgggcc
     1261 tgagggacaa attgcgagcg taccccgcgc agctttccgg cggacaacag cagcgcgtgg
     1321 cgattgcccg cgccgtggcg atggaaccca aagtgatgct gttcgacgag gcgacctccg
     1381 cgctcgaccc ggaactggtc ggcgaagtgc tgggcgtgat ggaagatctc gcccacagcg
     1441 gcatgaccat gctggtcgtc acccacgaga tgcgctttgc ccgggaggtc gggcatcgca
     1501 tcgttttcat ggacaaaggc gtgatcgtcg agcaaggcgc gccgaagcag attttcgagc
     1561 agccgcgatc ggagcggctg aaggcatttc tcggtcattt caccaagacg acgtgaccac
     1621 cgataacagc ggactcacag aggagacaag ccatgccaaa gcaacgaatt tcgaccacca
     1681 ccggcgcgca gccggtcggc gcctactcgc aggggttgtg cgccggcgac ttcgtattcg
     1741 tgtccggcca ggggccgctc gacccaaaaa ccggcaaggt cgtcggcaat accatcgagg
     1801 agcagaccgt gcgcgtgctg gaaaacatca aggccatcct cgaggcgggt ggcgcgagca
     1861 tggccgacgt cgtcaaggtc agcgcgcatc tgagcgatat gtcgctgttc gagcgttaca
     1921 accgcgcgta tgcgagttat ttccccgatc ccaaaccgac gcgcaccacc gtcggcagcc
     1981 aactgcctgg cattctggtg gagatcgacg ccatcgccta cctggggaaa tagcggaacc
     2041 ttccgcggca gccgtggctg aggtacgctt cgaacccacc aaagagacac gccatcgggg
     2101 aatgacatga ccaagagaaa aaaaccggaa gaactgcgca gccagcgctg gttcggcgcc
     2161 caggacctgc gctcgttcgg ccaccgctcg cgcgccctgc aaatgggcta cacgcgcgag
     2221 gactataagg gtaaaccggt catcgcgatc gtcaatacct ggagcgacgt caaccactgc
     2281 cactcgcacc tgcgcgagcg cgcacaggaa gtgaagcgcg gcgtgtggca ggcgggcggc
     2341 ttcccgctgg agatgccggc gatctccctg gcggaggtgt tccagaaacc gaccaccatg
     2401 atgtaccgca atttcctggc gatggaaacc gaggagctgc tgcgctccta cccggtggac
     2461 ggttgcgtgc tgatgggcgg ctgcgacaag accactcccg gcctgctgat gggtgcgatc
     2521 agcatgaacc tgccggcgat ctacgttccg gccgggccga tgctgcgcgg caactggcag
     2581 ggaaaggcgc tcggctcggg atcggatgtg tggaaatact gggacgaatt gcgcgccggc
     2641 aacatcaccg atcgcgactg gcgcgagatc gaggaaggca tatcgcgttc ccccggcacc
     2701 tgcatgacca tgggcacggc gtccacgatg accagcgtcg ccgaggcgct gggtttcagc
     2761 ctgcccaatg cctcgtcgat tcccgcggtc gattccaacc acagccgcat ggccgagtac
     2821 agcggcaggc gcatcgtcga gatggtgtgg gaggacctga aaccgcgcga catcctcagc
     2881 gaaaagtcct tcgacaacgg gatcaaggtg ctgatggcgc tcggcggctc gaccaacgga
     2941 ctgatccacg tgattgcaat ggccggccgc gccggcattc gcatcccgct cgagcgcttc
     3001 gaggaagtcg gccgtcacgc gccctatctg gtcaacatga ggccgtcggg caagtacctg
     3061 atggaggatt tctattacgc cggcggcttg gtcgggatgc tggaaggcat gcgcaaactg
     3121 ctcgataccg acgtgctcac ctgcaccggc aagacgctcg gcgaaaacat cgccggagca
     3181 atgatccaca accccgacgt gatccgcacg ccggaaaatc ccatgcaggc cgagggcggg
     3241 ctggtggtgc tgcgcggcaa cctcgcgccg gacggcgcag tgatcaagtc caccgccgcg
     3301 gacccgaagc tgttgaaaca cgcgggccgc gccatggtgt tcgacgacta caacgacatg
     3361 gcggcgcggt tcgatgatcc gaatctcgac gtcgacgccg gctcggtgct ggtgctgcgc
     3421 aacgccgggc cgctcggctg gccgggtatg cccgaatggg gcatgctgcc gatcccgaag
     3481 aagctgctca agcagggcgt gcgcgacatg gtgcgcattt ccgacgcgcg catgagcggc
     3541 accagctacg gcacttgtgt tttacatgtg gcgccggaat cctgggtggg cggtccgctg
     3601 gcgctggtga agaccggcga catcatcgaa ctcgacgtgc cggggaggaa actcacgctg
     3661 aaggtgagcg acgcggaact ggcgaaacgc cgcgccgcgt ggaagcccca tccgccgaag
     3721 ttccagcgcg gctacggcaa gattttcgcg gagcacatca agcaggccaa cctgggctgc
     3781 gacttcgatt tcctggaagg cacgacgccc acacccgagc cggagattca ctagagccgg
     3841 attgaaccat ccttcagtcg cgcttgccgg ttgccggatc ggggtggatg ttgtccaccc
     3901 agtgcacttc ttccggcttc tccaccgcgg gaatgtcgag gttgaccacc accggctcct
     3961 ggccgctgcg gcacagcacg caggacagcg gctcgctgtc ggaggcgttg atctcctggt
     4021 gcggaacgta aggcggcacg tggatgaaat cgccggggcc ggcctcggcg acgaactcga
     4081 gctggtcgcc ccagcgcatc cgcgccttgc cgctgacgac atagatgacg ctttccaccg
     4141 ggccgtggtg atgcgcgccg gtcttggctt tgggatggat gaccacggtg ccggcccaca
     4201 gcttctcggc accacagcgc gcgtaggtga tcgcagcggc acggttcatc cccggcgtct
     4261 gcgcggtatt ggtatcgact tcggatccct tgacgatacg gatgccgtgg tacttccact
     4321 tcgattcggc cattttccct tcccttctca cctgagctgc agcagggcca acagcaacac
     4381 ggtcgggatg atggtgttca ccagcgacag catgaacaga ttgcgcgggc ctttctcgtt
     4441 cgatacggca tgaatgaacg cggtgacccc cgataccccg accaccagcc cgccgatcgc
     4501 ataaagcacg gaaaaaatct ggaactctcc ctggcgctgg aaatagggca cgagattgag
     4561 ctgccaggtc aggtagacga tcagccccag attggcaagc agcggcaggt agtagagaag
     4621 aaaccgcctg agattcagct tcaccgcttc ctttcctcca atgacatctg caacacacgc
     4681 gcaacatgca gcgcgttcct gcgcgcgccg gcgtgaatct ggtgccggca actcgtgccg
     4741 tccgcgacga tgatcgtatc cgggcccgct tttctgacgg ccgggagcag cgactgttcc
     4801 gccatcttca tactcaccgc gaaatgttcg gcctcgtagc cgaaactgcc ggccatgccg
     4861 cagcagccgg aatccaccgt ctgcactttc aggccgggaa tcatgcccag caccttgcgt
     4921 accgcaggca tggcgtcgaa ggctttctgg tggcagtgcc catgcagcag ggcggtctgc
     4981 gtccgcagcg gcgccaggtc caacttgagg cgcccggcgt ccgcctcgtc ggcgagaaac
     5041 tcctcgaaca gcaacgcacg ctcggcagtc ggcccgattt ctccgtccag tcccatcacc
     5101 aggaactcgt cgcgcaacgt cagcaggcac gaaggctcaa gcccgacgat gggcacgccg
     5161 cgatcgacat agggacgcag cgcgtcgagc atgcgctgcg cctcaaccct tgcctcgtcc
     5221 accaggccgg cagcgagaaa tgtgcggcca cagcaaagcg ggcgaccgcc gtcgcgcgct
//
LOCUS       DASQOP010000038        27184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_819559, whole genome shotgun sequence.
ACCESSION   DASQOP010000038 DASQOP010000000
VERSION     DASQOP010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 27184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27184
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819559"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..475)
                     /locus_tag="VE030_01380"
     CDS             complement(134..475)
                     /locus_tag="VE030_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HZF22099.1"
                     /translation="MSNRGTWAEDAAAAFLVKRGLKLLERNYRCRFGEIDLVLRDART
                     LVFVEVRYRRSHSFGGAGESITTSKRKKLLRAARHYMVAHGEFPACRFDAVLLNGDTP
                     EVDWVVNAFGE"
     gene            complement(472..1575)
                     /locus_tag="VE030_01385"
     CDS             complement(472..1575)
                     /locus_tag="VE030_01385"
                     /inference="COORDINATES: protein motif:HMM:NF016251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HZF22100.1"
                     /translation="MQLRPALSLLVPALLYWALIALSTGLVFAQESENTGAPSAHAHI
                     AVILPLQSTSFGKHADSVRLGILAAAGLMQENGPGVTVYATTEETDQILTAYQRAVTR
                     GARAVIGPLTRNGVTALALSGVVSVPTLALNIPDGDILMPRDLYSFGLQIESEARQVA
                     QLAFSNGVRRAFVVAGETALGGRIAQAFVDEWKKLKAEIAGQFVYSTEGAALIRLREQ
                     VTTSKADVVFLSLDATRARFIRSYLGGSLPIYATSLVFASNTDTLANFDLEGVRFLDM
                     PWLLQPDHPAVMSYLKPDVQRSGLDQERFYALGIDAYRIVQELLRPYEPMVSLDGVTG
                     TITPTDTQQFARSLVPAQFSQGRAKPVEASPMR"
     gene            1570..2430
                     /gene="rsmI"
                     /locus_tag="VE030_01390"
     CDS             1570..2430
                     /gene="rsmI"
                     /locus_tag="VE030_01390"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HZF22101.1"
                     /translation="MHSEDTKLTEPTLYVVATPIGNLRDMTLRALDVLKSVDSIAAED
                     TRVTRKLLGHHGIHVKLFALHEHNERDAARRLIAALSEGKSVALVSDAGTPGISDPGT
                     VAVAQVREAGFRVVPIPGPNAAIAAIAASGMRAATFCFHGFLPARKGDRETALARLGR
                     QTGLLVFYEAPHRIEASVESMVAVFGGERRIGIARELTKLFEQVHVCRLAEASAWLQQ
                     DEDHRRGEFVLLVEGCAEDAAQPEDDSSRVLALLLDELPLKQAVKLAAEITGARKNAL
                     YQRALELKKE"
     gene            2467..3495
                     /gene="pyrC"
                     /locus_tag="VE030_01395"
     CDS             2467..3495
                     /gene="pyrC"
                     /locus_tag="VE030_01395"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HZF22102.1"
                     /translation="MQITITKPDDWHLHLRDGDAMKAVLPDTAQRFARAIVMPNLRPP
                     VTTTAAAGAYRDRILAALPAGSSFLPLMTLYLTDNTRPQEIRAAKSGGFVHAMKYYPA
                     GATTNSDAGVTDIRKCFDTFEAMAEIGMPLLIHGETTDTDVDLFDREKVFIERTLIAL
                     VQRFPRLKIVVEHITTTNAVKFVLGAPNTVAATITAHHLLLNRNDLFRGGIRPHHYCL
                     PVLKREEHRQALVKAATSGIPKFFLGTDSAPHARHAKETGCGCAGIYSAHGGIELYAE
                     AFEAAGALDKLEGFAAFFGADFYGLPRNRERITLNKEAWPVPAELRYGADTLVPLRGG
                     ATIAWRVA"
     gene            3791..4072
                     /locus_tag="VE030_01400"
     CDS             3791..4072
                     /locus_tag="VE030_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HZF22103.1"
                     /translation="MNKAELVEAIAKKTGASKALADTMIDAFIDAVTVSLKKGESVTL
                     VGFGTFSQGKRAARTGRNPQTGAALKIPAARTAKFAAGAKLKSAVNGRK"
     gene            4565..5025
                     /gene="rnpB"
                     /locus_tag="VE030_01405"
     ncRNA           4565..5025
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VE030_01405"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            5315..5758
                     /gene="mraZ"
                     /locus_tag="VE030_01410"
     CDS             5315..5758
                     /gene="mraZ"
                     /locus_tag="VE030_01410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HZF22104.1"
                     /translation="MFQGATALNLDVKGRLAVPTRHRDGLQSGDGSKQVLTAHPHRCL
                     LLYPAPAWEPIRAQIMGFSSFDPQTSLWKRLLVGFAEEVELDASGRLLVSPELRKFAG
                     LEKQVMMVGQGSHFEIWSQSTWEKQLEQLAIGGDKLPPGMENFAL"
     gene            5777..6700
                     /gene="rsmH"
                     /locus_tag="VE030_01415"
     CDS             5777..6700
                     /gene="rsmH"
                     /locus_tag="VE030_01415"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HZF22105.1"
                     /translation="MRSHQTVLLEEAIEALRIKADGIYVDGTFGRGGHSRAILARLGS
                     SGKLIVMDRDAMAIAAATEIADPRLAVFHAQFGALREVVRLAGVAHVDGVLFDLGMSS
                     AQLDEADRGFSFRFDGPLDMRMDRSRGQTAAQWLEQSSEREIGEVIKRYGEERFAKQI
                     ARAIVAARSRGPVDRTRQLAEIVAKAVPTREPLQDPATRTFQAIRIHVNQELEELAVM
                     LPRCLEVLSDGGRLVVISFHSLEDRIVKQFMRTHSLPGALPARLPLRARELPKPYLRL
                     IGKPVFPSESEIAANPRARSAVLRVAERIAA"
     gene            6697..6978
                     /gene="ftsL"
                     /locus_tag="VE030_01420"
     CDS             6697..6978
                     /gene="ftsL"
                     /locus_tag="VE030_01420"
                     /inference="COORDINATES: protein motif:HMM:NF016860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HZF22106.1"
                     /translation="MIKLNLVLSGILVVCALGLVTSQHKARKLFVELQKQQEVAKQLE
                     VEYGQLHLEQSTWAMHSRVEKIAADTLRMRVPPAARVHLVPPPPAPSNP"
     gene            6975..8705
                     /locus_tag="VE030_01425"
     CDS             6975..8705
                     /locus_tag="VE030_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HZF22107.1"
                     /translation="MRPSLNPALSVRLPVWRARLLLLLVFCGLMLLAARAFYLQGLHN
                     DFLQQKGETRYARVVEISAHRGMVTDRNGEPLAVSTPVESVWASPADADLSTEQRVKL
                     ARLLGTDATELRRRLSDSEREFVYLKRQLPPEQAAKVVQLNLPGVFLQREYRRYYPAA
                     EVTAHVVGFTGVDDNGQEGIELAYQEWLAGKRGSRRVIKDRLGRIIEDIESIRVPQQG
                     RDLALSIDQRIQYLAFREIKSAVAEHEAKAGSIVVLDATSGEVLALANWPTYNPNNRD
                     TLKMARSRNRAVVDLFEPGSTLKPFTAAAALESGLVGPGSVIDTEHGRFTIGKRTIRD
                     VHPEGFLTVSQVIQKSSNVGSAKIALAMAPQKLWAIFSAVGFGTQTRVGFPGEATGRL
                     RAYQSWKPIEHATMSYGHGVSVSLLQLARAYSVFATDGELKQLTLIRRDQPVEGKRVV
                     SRRTAQAVRKMLEMVTQPGGTATRAQVAGYRVAGKTGTAHKLDGATYAADRYVSSFVG
                     FAPASSPRLVIAVMIDEPGGKYYYGGEVAAPVFSNVMAGALRLLGVTPDAPPDNVIPP
                     GSAPLIGEEV"
     gene            8702..10198
                     /locus_tag="VE030_01430"
     CDS             8702..10198
                     /locus_tag="VE030_01430"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HZF22108.1"
                     /translation="MNAAARSPQPGQRAFDVRDIDALGVPVTGLAVDSRKVKPGDLFL
                     AYPGEHADGRRFIDQAIGAGAAAVLWDSRSFLWNSAWKLPNLGVPKLRTHLGEIASDF
                     YGHPSRHLWIAGVTGTNGKTSCAHWIAQSLSRLGRKTAVIGTIGSGFPGALEPALNTT
                     PDAVALHARLADFRMHRAEACAMEVSSHGIEQGRVGGVEFDVALFTNLSRDHLDYHKT
                     MEDYAAAKAKLFHWPQLKHAIVNLDDRFGQQLAGSIDRSRVNVLGYGLGRGEIAGHRL
                     DLSTRGLKLEITTPWGAAELESPMIGGFNASNLLGVLGVLITSDVPLQDAVQALSRVE
                     PVPGRLQMVREPGAPLVVVDYAHTPDALEKVLETLRFTLSNEGKLHCVFGCGGDRDPG
                     KRPLMGEVATRLADRVVITSDNPRSENPRAIIDEILAGAHPKYAIEEDRAAAILQAIS
                     SAAPQDVVLLAGKGHETYQEIGGRRLPFDDAEVAREILQRLPRRLGHA"
     gene            10191..11573
                     /gene="murF"
                     /locus_tag="VE030_01435"
     CDS             10191..11573
                     /gene="murF"
                     /locus_tag="VE030_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004538784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HZF22109.1"
                     /translation="MLDLVSAAQALGARRVGGNAEFVRVTTDSRDIRPGDLFVGIRGE
                     RFDGQEFAAQALAAGAVGAMVSDGSRNAVAGARLLVVPDTRIALGQLAAYWRARFAMP
                     LIAITGSNGKTTVKEMLAAILRQSAGESGVLATVGNFNNDIGMPLTLLQLRAQHRYAV
                     VEMGMSHLGEIAYLSRLAKPTAALINNAGTAHIGELGSTEAIARSKGEIFEGLDMAGT
                     AIINSDDAFADFWRGLAKPRRVVDFGLDRKAAVLARYELAEAGSLVTIVTPESEFVAT
                     LNVPGLHNVKNALAAATSAYALGIESRAIAAGIAAYRGVKGRLQRKRLPSGATLIDDT
                     YNANPESMKAAIAVLAAHAGRNVFVMGDMGELGDAASAMHAAIGVFAKRAGVQRLFAL
                     GELSAEAVRGFGKGGAHFATIEDLIAAITGVLGPNTTLLIKGSRFMRMERVVEALGAV
                     NGEQTAKGGA"
     gene            11573..12658
                     /gene="mraY"
                     /locus_tag="VE030_01440"
     CDS             11573..12658
                     /gene="mraY"
                     /locus_tag="VE030_01440"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HZF22110.1"
                     /translation="MLLLLTQWLAEDVRAFNVFNYITLRAVLATLTALLISFVVGPAM
                     IRKLTAYKIGQSVRDDGPKTHLSKAGTPTMGGTLILVSITITTLLWADLRNRYVWVVL
                     AVTLGFGMVGWVDDYRKVVHRDPKGLSARGKLLWQSLIGLAAAVYLATSATLPAQTEL
                     IVPFFKQVAYPLGIIGFVVMTYFVIVGTSNAVNLTDGLDGLAILPTVMVGSALGVFAY
                     VAGNAVFSKYLGFPSIPGAGELTVICAALAGAGLAFLWFNAYPAEVFMGDVGALALGA
                     ALGTIAVVVRQEIVLFIMGGVFVVETLSVMLQVASFKLTGKRIFRMAPLHHHYELKGW
                     KENQVVVRFWIITMMLVLVGLSTLKLR"
     gene            12797..12982
                     /locus_tag="VE030_01445"
     CDS             12797..12982
                     /locus_tag="VE030_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22111.1"
                     /translation="MTSRLQFELFMREMRAQAANKGPKQLPLANLHPEQTEDVGALRL
                     SRIEAAMKETRAARAGK"
     gene            12960..14342
                     /gene="murD"
                     /locus_tag="VE030_01450"
     CDS             12960..14342
                     /gene="murD"
                     /locus_tag="VE030_01450"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HZF22112.1"
                     /translation="MPLELENRRVLVLGLGSSGMSMARWLAGRGARVHVADSRPVPPN
                     ADQLREELPDIDLHTGAFRPESFADAELIAISPGVPLAEPLVQAALARGLEVIGDIEL
                     FARVRDEFPGAKVIGITGSNGKSTVTEMVGTMTRAARTRTLVAGNIGLPILEALWEIE
                     AGRRRRPDVFVLELSSFQLETTENLNVDAGTVLNLSEDHLDRYAGIREYAQAKARIFF
                     GNGAQVINREDAWAREMAASGRRVFTFGLDEPREEGAWGLRNLDGELWLAEGRANLMK
                     VSELKVTGLHNAANALAALALCRAVGFPYEALLDALFSFSGLPHRVQRIVDINGVAFF
                     DDSKGTNVGATVAALEAMGRKVVLIAGGDGKGQDFSPLRDAVARHARAVVLIGRDRDR
                     IAAAIESTGVVLVRASDMHEAVRATLALAHAGDAVLLSPACASFDMFRDYEHRAQVFV
                     DEVRALEGAQ"
     gene            14339..15508
                     /gene="ftsW"
                     /locus_tag="VE030_01455"
     CDS             14339..15508
                     /gene="ftsW"
                     /locus_tag="VE030_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006682491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HZF22113.1"
                     /translation="MNGVLGHVANPPAPIAELDYAVVSLAALLLGTGLVMVYSSSIAI
                     AEAGHYAGNQPAYFLIRHSVFVFVGVMAGFICFQFPTRVWQQAAPYLFVLGVLLLLLV
                     LIPGIGKEVNGSQRWLSLGMFNLQPSELMKLLVLLYAADYTVRKAALMHSFRKGFLPM
                     LLVMLLVGGLLLREPDFGAFVVITVIAMGVLFLGGMNWKLFAVLIMLLIIGFVLLIWS
                     SPYRLQRVMGFMDPWADPYGKGYQLSHALIAFGRGEWFGVGLGASVEKLFYLPEAHTD
                     FLLAVIAEELGFAGVIAVIALFGWLTWRAFAIGNQAARLERYFSALVAQGVGLWIGAQ
                     AAINMGVNMGVLPTKGLTLPLLSFGGSGIVANCCALAILLRVDYENRQMMKGLSL"
     gene            15514..16590
                     /gene="murG"
                     /locus_tag="VE030_01460"
     CDS             15514..16590
                     /gene="murG"
                     /locus_tag="VE030_01460"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HZF22114.1"
                     /translation="MSDSGNARTILVMAGGTGGHIYPALAVADFLRQQGWKVVWLGAR
                     SGMEAMLVPQHGYAVEWIRFSGLRGKGIVRLALLPLNLLIAFWQSARAIFRVRPDVVL
                     GMGGYIAFPGGMMAALLDRPLVLHEQNSIAGLANKVLAQVADRILVAFPGALKKSAWT
                     GNPVRREIAELPAPEQRFRDRSGNLKVLVVGGSLGAQILNDTVPRALALIPQTRRPDV
                     THQAGEKHITTLEKNYRDAGVKATSLAFIHDMAGAYADADLVICRAGALTVAELAAAG
                     VASILVPFPHAVDDHQTHNARFLSDKSAAVLLPQKELTPRKLADLLLGFTRERILEMA
                     KLARSLGKPDATRVVAEHCMAAAG"
     gene            16714..18153
                     /gene="murC"
                     /locus_tag="VE030_01465"
     CDS             16714..18153
                     /gene="murC"
                     /locus_tag="VE030_01465"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HZF22115.1"
                     /translation="MKHKVKHVHFVGIGGAGMSGIAEVMLNLGFEVSGSDIADSAAIQ
                     RLKALGAKVHIGHADANVSGANAVVTSTAVKPDNPEVAAARARRIPVVPRAMMLAELM
                     RLKQGIAVAGTHGKTTTTSLVASVLAEADMDPTFVIGGRLEAAGSHAKLGSGDFIVVE
                     ADESDASFLYLQPVLAVVTNIDADHMETYGHDFSKLRQAFLEFIEHLPFYGMAVLCID
                     DANVRSLMPLITRPVTTYGLADGAQIRAVDVEAAAGQMRFRVVRKIGFNGHAQAAPDL
                     RITLNLPGLHNVQNALAAIAVGMEIGAHDAAIVKALAEFRGVGRRFQRYGDVALRNGG
                     SFTLIDDYGHHPVEMAATIAAARGAFPGRRLMLAFQPHRYTRTRDCFEDFVKVLSSVD
                     ALLLTEVYPAGEAPIVAADGRALARAVRVAGKVEPVFVEHVNELGAAILASVQPGDVV
                     ITMGAGSIGGVPGKMARMKDEANADYRHR"
     gene            18159..19103
                     /gene="murB"
                     /locus_tag="VE030_01470"
     CDS             18159..19103
                     /gene="murB"
                     /locus_tag="VE030_01470"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HZF22116.1"
                     /translation="MDMSEQERLGTPALRGELRRDEPMRKRTSWRAGGTADRAYLPAD
                     LDDLCVFLRGLPGAEPVFFAGLGSNLLVRDGGIRGTVVFTHAALRRFFLDEGAGAAEI
                     CAEAGVACPKVARFAAVHNLEGAEFLAGIPGTVGGALAMNAGCYGAETWDVVARVRTV
                     DRCGRLQIRSPNEYSIGYRHVIPLQAQDEFFVAAWFRLRHGDGGRGRQTIRNLLSRRI
                     ASQPLDEPNAGSVFRNPPGDHAARLIESCGLKGKRIGAAEVSLKHANFIVNKGDATAK
                     DIEELITLVQETVKRKTGVELEREVQIVGEARGEGRKA"
     gene            19100..20017
                     /locus_tag="VE030_01475"
     CDS             19100..20017
                     /locus_tag="VE030_01475"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HZF22117.1"
                     /translation="MRAKNFGKVAVLLGGRSAEREISLMSGNAVLAALARRGVDAYAF
                     DTRDRDLHELRSQEIDRAFIALHGRYGEDGTVQGALELMGIPYTGSGVMASSLAMDKW
                     RTKLVWIAAGIPTPRFVMLEAGSDFKAVANELGLPLVVKPSREGSTIGLTKVTGAQSM
                     QAAYLKAAEYDDMVMAEEFIAGAEVTASILREQTLPLIRIEAPDSRYDYQAKYFSDET
                     RYFCPSGLPPAQERRIQDLALKASRILDCRGWGRVDVMLDAKGEPYLLEVNTVPGMTG
                     HSLVPMAAKGAGIPFDDLVVRILEDAHVG"
     gene            20007..20726
                     /locus_tag="VE030_01480"
     CDS             20007..20726
                     /locus_tag="VE030_01480"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HZF22118.1"
                     /translation="MWDNAAVLNRAANLLYGAAALLIVYGCIHYVVHLPVFPLREIRV
                     IGDVGHVTNEQVAAVTARELRGNFFTVDLAQAREAFEKLPWVRTVNVRRQWPDRLEVA
                     VEEHQPLARWGSTALVNAHGEVFEAAISSKLPVFFGPEGTAVEVVARYAEFDRLLAPI
                     GRKVVLITLSARRAWQLRLDDGTVLQLGRENLEARLAGFVSAYPRTVARLPQPPSHVD
                     LRYSNGFAVRTPGLKWDARKA"
     gene            20748..21992
                     /gene="ftsA"
                     /locus_tag="VE030_01485"
     CDS             20748..21992
                     /gene="ftsA"
                     /locus_tag="VE030_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HZF22119.1"
                     /translation="MALSGRKESKNLIVGLDIGTSKIAAIVAEMKPEGGFEVIGMGSS
                     PSRGLKKGVVVNIESTVNAIQRALEEAELMADCKIREVWTGIAGSHIRSFNSHGMVAI
                     KDREVAQTDVDRVVETAKAIPIPTDQQILHVLNQEFVIDGQEDVREPLGMSGVRLEVK
                     VHIVTGAVSAAQNIMKCVRRCGLEVNDLILQPLASATAVLSEDERDLGVCLVDIGGGT
                     SDIAVFTQGAIRHTAVIPIAGDQITNDIAMALRTPTKEAEEIKRRYGCALRELANAQE
                     MVEVPGVGDREPRKMSRQTLAEVIEPRVEELYSLVQAELRRSGFEELLSSGIVITGGS
                     SSMQGMVELGEEVFHMPVRLGLPGYAGGLSEVIRNPRYSTGVGLLMAGLNQHHRHQIE
                     KMQSGSFKQIFGRMKKWFETNF"
     gene            22058..23215
                     /gene="ftsZ"
                     /locus_tag="VE030_01490"
     CDS             22058..23215
                     /gene="ftsZ"
                     /locus_tag="VE030_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HZF22120.1"
                     /translation="MFEIVDTQPQEAVIKVVGVGGCGGNAVDHMINEGVEGVEFICAN
                     TDAQALKRNKAKTLLQLGPNVTKGLGAGANPDIGREAAMEDRERIIELIEGADMLFLT
                     AGMGGGTGTGAAPIVAEAARELGILTVAVVTKPFAFEGKRQKIAQQGLDELCRHVDSL
                     IVIPNDKLMQVLGEDVSMLDAFKAANTVLHGAVAGIAEVIKCPGLVNVDFADVRTVMS
                     EMGMAMMGSAMAAGADRAHCAAEQAVASPLLEDVNLAGARGVLVNITASGTLKMKEVH
                     EVMNTIKGFTAEDATVIVGTVIDEAMNDRLRVTMVATGLGGAAAVNRHHQKPLTMVKT
                     GTDAGPLAVNYGELDAPAVMRRRSRDAAVEAMKQSGVDMLDIPAFLRKQAD"
     gene            23361..24272
                     /gene="lpxC"
                     /locus_tag="VE030_01495"
     CDS             23361..24272
                     /gene="lpxC"
                     /locus_tag="VE030_01495"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HZF22121.1"
                     /translation="MIKQRTLKNIVRATGIGLHTGEKVYLTLRPAAPNTGIVFQRVDL
                     EPVVDIKADPYAVGDTRLSSCLERAGARVQTVEHLMSALAGLGIDNVYVDLTAGEVPI
                     MDGSASPFVFLLQSAGIDEQAAAKKFIRIKKPVELKQGDKWVRFEPYNGFRVDFSIDF
                     KHPVFASSNKRVVVDFEHTSYIKEVSRARTFGFMQDVENMRAQGLALGGSLDNAIVMD
                     EYRVLNADGLRYEDEFVKHKALDAIGDLYLLGHPLIGAYTAYKSGHALNNGLLRRLME
                     DATAWEFVSFLSPEEAPAFLRLQIQAA"
     gene            complement(24516..24962)
                     /locus_tag="VE030_01500"
     CDS             complement(24516..24962)
                     /locus_tag="VE030_01500"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HZF22122.1"
                     /translation="MTSERIGLLIDRLPQLQSLNRQVRRLIALQDLLTEVLPDSLASS
                     TTVALSAAEELVLFADNGAAAAKLKHLAPRILVFFRQRRHEVTAIRVQVQVRIRHNPL
                     LQKRISLSQTARQAISELSATLDVSPLKSALERLARRKTPLPDRHD"
     gene            25000..25917
                     /locus_tag="VE030_01505"
     CDS             25000..25917
                     /locus_tag="VE030_01505"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZF22123.1"
                     /translation="MNIILVSNKFAQARSFAVNTPQVIFLGLLLGLLVVSLAVLMNYF
                     SLRYAVTNQSPYLQSLLEAVQAEQTRKTQSYLRESLNTMAARLGEMQAKLQRLDTLGE
                     RLTKLAGIKPQEFMFDQAPARGGAVSTIPSKELSMGELGENLDRLAQQVDDRSDKLGV
                     LESMLTLVHAKQQLLPTQLPVNTGWYSSNFGWRIDPFTGWNAFHEGMDFMAQEGEAVT
                     AAAGGVVVFSDPHPQYGNMIEIDHGNGLVTRYAHASKLIAKVGDVVLRGQKIAEVGNT
                     GRSTGTHLHFEVRQWGAPQNPARFLQVPG"
     gene            25995..>27184
                     /locus_tag="VE030_01510"
     CDS             25995..>27184
                     /locus_tag="VE030_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZF22124.1"
                     /translation="MFTNPLKKIFGSRNDRLLRKYAHSVQAINALEPGLREVSDAELR
                     ARTEVFKQRVQNGETLDDLLVEVFATVREASRRVLNMRHFDVQLLGGIALHNGKIAEM
                     RTGEGKTLVATLPAYLNALSGRGVHVVTVNDYLANRDAEWMGRVYRFLGLSVGVNLSQ
                     MEHELKQQAYAADITYGTNSEFGFDYLRDNMVFQASERVQRGLTYGIVDEVDSILIDE
                     ARTPLIISGQADDSTDLYYRMNEIVPTLAQVKEENGPGDFTVDEKAHQVLLTEAGHER
                     AEDLLTKAGLLPENGSLYDAANIALMHHLYAGLRAHSLFHRDQHYVAQNDEIIIVDEF
                     TGRLTPGRRWSDGLHQAIEAKEGVRIQRENQTLASITYQNYFRMYKKLAGMTGTADTE
                     AEEFA"
BASE COUNT         5260 a         8033 c         8857 g         5034 t
ORIGIN      
        1 ctgcagtttc aagtgggcgc tgtcggcgaa atgtttgttg atccggctct tgaggtccat
       61 cctgaaaata ggctgcattc ccgctacagg cgaattattg cacaaagaag gtgcgggctc
      121 cgtcatatga tgctcattca ccgaatgcgt tcacgaccca gtcgacctcg ggtgtgtcgc
      181 cgttcagcaa cactgcatcg aaacggcagg ctgggaactc gccgtgtgcc accatgtaat
      241 gtcgcgctgc ccgcaggagt tttttgcgct tgctcgtggt aatgctctcg ccggcgccgc
      301 cgaaggagtg gctcctgcga tatcgcactt cgacgaaaac cagtgtccgg gcatcgcgca
      361 gtaccagatc gatttcaccg aaacgacagc ggtagttgcg ctccagaagc ttgagaccgc
      421 gctttaccag gaaggccgca gcggcatctt cggcccaagt tccgcgattg ctcatcgcat
      481 aggggacgct tcaaccggtt tggcgcggcc ctgggaaaac tgcgccggta ccagtgagcg
      541 ggcaaattgc tgggtgtcgg tgggcgttat cgtgccggtc acgccgtcca gagaaaccat
      601 cggctcgtac gggcgcaaca actcctgcac aatccggtat gcgtctattc caagcgcgta
      661 gaaccgttcc tgatcgagcc ccgacctttg cacgtcgggc ttcagataac tcatcaccgc
      721 gggatggtcc ggctgcagca gccagggcat gtcgaggaaa cgcacacctt ccagatcgaa
      781 gttggccagc gtatcggtgt tggacgcgaa taccagcgaa gtggcataaa tgggcaggct
      841 gccgccgaga tacgaacgaa taaagcgcgc ccgcgtcgcg tcgagagaga ggaaaacgac
      901 gtccgctttc gaggtcgtca cttgttcgcg cagcctgata agcgcagcac cttccgtgct
      961 gtagacgaac tgccccgcga tctccgcctt cagtttcttc cattcgtcga cgaaggcttg
     1021 cgcgatccgg ccgccgagcg cggtctcgcc ggccacgacg aaggccctgc gcacgccgtt
     1081 actgaatgcg agctgcgcaa cttggcgtgc ctccgactcg atctgcaggc cgaacgagta
     1141 caggtcgcga ggcatgagta tgtcgccgtc gggaatattg agtgccagcg tgggcacgct
     1201 gaccacgccg cttagtgcga gcgcagtgac gccgttgcgg gtaagcggcc cgattaccgc
     1261 acgggcgccg cgcgttaccg cgcgctggta tgcggtgaga atctggtccg tttcctctgt
     1321 ggtggcgtac acggttactc ccggtccgtt ttcctgcatc aatccggcgg cggcgagaat
     1381 tccaagacgc accgagtcgg cgtgtttgcc gaaagaggtt gactgtagcg gcagtattac
     1441 cgcgatatgc gcgtgcgcgg atggggcgcc ggtgttctcg gattcctgcg cgaagactag
     1501 gccggtggat aaggcgatga gcgcccagta tagtaacgcc ggtaccaaca gcgaaagggc
     1561 cgggcgaagt tgcatagtga agacaccaaa ctgactgaac ccacattata tgtggtggcc
     1621 acgccgatcg ggaacctgcg cgacatgacg ctgcgcgcgc ttgacgtgct gaagtccgtg
     1681 gacagcattg ctgccgaaga cacgcgagtc acgcggaaat tgctcggcca ccatggcatc
     1741 catgtgaagc tgtttgcgct gcacgaacac aacgagcgcg atgcggccag gcgtctgatc
     1801 gcggctctct cggagggaaa atcggtggcg ttggtatccg atgctggcac gccaggaatc
     1861 agcgatcctg gaacggtggc agtggcacaa gtgcgcgaag caggctttcg cgtggtgccg
     1921 ataccagggc cgaacgcggc catcgccgcg attgcggcat cgggaatgcg ggcggcaacg
     1981 ttctgtttcc acggtttcct gccggccaga aagggcgatc gcgaaactgc gctggcgcgg
     2041 ctcggtcgcc aaaccggcct gctggtgttc tacgaggccc cgcaccggat cgaggcctcg
     2101 gtggaaagca tggtggccgt gttcggcggt gagcgtcgca tcggtatcgc acgcgagctt
     2161 accaagctgt tcgaacaagt tcacgtctgc cggcttgccg aagccagcgc ctggctgcaa
     2221 caggacgagg atcaccgccg cggcgagttc gtgcttctgg tggaaggttg cgccgaagac
     2281 gccgcgcagc cggaggacga ttcgtcgcgc gtgctcgcac ttctgctcga cgagctgccg
     2341 ttgaagcagg cagtgaagct cgccgcagaa atcaccggtg ccagaaaaaa cgcgctctac
     2401 cagcgcgcgc ttgaactcaa gaaggaataa gacaggaatt cttcccgact atccgccatt
     2461 gacttcatgc agatcaccat caccaagccc gacgactggc acctgcacct gcgcgacggc
     2521 gacgcaatga aggcggtgct gccggacaca gcgcagcgct tcgcccgtgc catcgtcatg
     2581 cctaacctca gacccccggt aacgacgacc gctgcggccg gcgcttatcg cgaccgtatc
     2641 ctcgccgctt tgcctgcagg cagcagtttc ttgccattga tgacgctgta ccttaccgac
     2701 aatacccggc cgcaggagat ccgcgcagca aagtcgggcg ggttcgtgca cgcgatgaag
     2761 tactacccgg cgggtgcaac gaccaattcg gatgcagggg tgactgacat ccggaagtgc
     2821 ttcgatactt tcgaggccat ggccgaaatc ggaatgccgt tgctgataca tggtgaaacg
     2881 accgacacgg acgtcgactt gttcgaccgg gaaaaggtct tcatcgagcg cactctgatc
     2941 gcactggtgc agcgattccc gcgactgaag atcgtcgtgg agcacatcac caccaccaac
     3001 gcagtgaagt tcgtgctcgg cgcgccgaat accgtggcag cgaccatcac ggcgcatcat
     3061 cttctgctca accgcaatga cctatttcgc ggcgggattc gcccgcatca ctactgcctg
     3121 ccggtgctca agcgcgaaga gcaccgccag gcgttggtga aggcggcaac ttccggcatt
     3181 cccaagtttt ttctcggtac cgacagtgcg ccacatgcgc gccacgcaaa ggaaaccggc
     3241 tgcggctgtg ccggaatcta cagcgcacac ggcgggatcg agttgtacgc ggaggccttc
     3301 gaggcggccg gagcgctcga caaactggag ggctttgccg ctttcttcgg cgcagacttc
     3361 tacggcctgc cgcgcaatcg cgagcgcatc acactcaata aggaagcctg gccggtgccg
     3421 gcggaactgc gctacggggc tgacaccctg gtgccgttgc gaggcggcgc tacgatcgcg
     3481 tggcgggttg cgtgacgcaa agaccttgaa agcactgccg gttgtagcgc gcgaacggtt
     3541 catttcggaa ctgagacgta tcgcgcatcg ggcgtcatga caggtggggc tatggtgcac
     3601 ggcgcgagtc gtggataatg gagcagggtg tgaggaggca tcgagggggg caagaacaat
     3661 gcccgtgcgg ttgcatcaac actcgtggtg catcgccgcg gacgtctcgc tcaatgcgtg
     3721 tgtgcgttga ccggatggcg accaaaacct aagcttgcgg ccgctgtacg tttagatgac
     3781 agggggataa atgaacaaag cagaactcgt cgaagcaata gcaaaaaaaa caggcgccag
     3841 caaagccctg gcagacacca tgatcgatgc attcatagac gccgtaaccg tatctctaaa
     3901 gaaaggcgaa agcgtgacct tagttggctt tggaaccttt tcccagggta agcgtgccgc
     3961 acgcacgggc cgcaatcctc aaaccggtgc ggcgctcaag atcccagcag ccaggacggc
     4021 gaagtttgcg gcaggcgcaa aactcaagtc ggccgtcaac ggcaggaaat aagattcaac
     4081 aagcaagaga aattggctac ccgaggggca aacgcccctc tttttttgcc tgccattctg
     4141 gcaatgctgc gttgcagtta aagcagacgg aacgaagccc tcgtcgcgcg aggtcttcga
     4201 cgagagcccg tgcagtcgct gcgtaattcg cttgcgcaga ggacaacatc gcggatgagg
     4261 cgccggcacg gcgaatcgag cgctgttgct gtgaaggctg actcttggcg gggattgtcc
     4321 aatcagagtc atgccgcaac taaagtccgg ggaccccggc tttccgatgc gataatccat
     4381 tccgaagctg gccgggcagt cgctgcgcaa ttcgcttgcg cagaggacaa tttcgcggca
     4441 ggccgccgca cggcggcggc aagcgatgtt gctgcgaagg ctgacattta gccgcgcagc
     4501 ggatcaaagt cacgccgcaa ctaaagtccg tggaccccgg ctttccgatg cgataatcca
     4561 ccccgaagct ggccgggcag tcgctgcaca attcgtttgc gcagaggaaa gtccgggctc
     4621 cgcagagcag gatgccggct aacggccgga cgccgcgagg cgatggaaag tgccacagag
     4681 aacagactgc cgatggccga cgcccgactt tgtggccgta aggccgcaga gtctcgctct
     4741 tgtagcgaga aagggcgtcg gatcaggcga tggtgaaacg gtgtgcgttc cgcaaggagc
     4801 gttggcggtc gcgaggctgt cgggtaagag cccaccgcgt ttctggcaac agaaacggca
     4861 cggcaaaccc catccggagc aagaccaaat aggggggcga tagcgcggct cgcgctgctc
     4921 ccgggtaggt cgcttgagcc ggcaggcaac tgcaggccca gatgaatggc tgccggggtg
     4981 ccgcaaggta ctccacagaa cccggcttac aggccagctt cgctctttcc cgcacgtcgg
     5041 tttcccgcca agaaattgtt tccccggatt ctatgtcgtg acgccctctg ccaattgatc
     5101 gaaataacat taacaatatg tcttaactat attattttta aaatgtaatc actttcagtc
     5161 tcaggtggtt tcaagagtcg cttgtaagtc tttgtcgtaa ctgaaaaaag ccggcgggtt
     5221 ccccttgacc tgctattttt tttccactag agtggggaaa agtagggttt tgtgggaaaa
     5281 cctggaaatt ccaatacttc cggcggaata acaaatgttt cagggggcaa cagcactgaa
     5341 cctcgatgtc aagggccgcc ttgccgttcc aacacggcat cgcgacggct tgcaatcagg
     5401 ggatggaagt aagcaagtac ttacggccca tccgcatcgc tgccttttgc tttaccccgc
     5461 gccggcgtgg gagccgatcc gcgcccaaat catgggtttc tccagcttcg atccccagac
     5521 cagtttatgg aagcggcttc tggtgggttt tgccgaggaa gtcgaactgg atgcctccgg
     5581 gcggctgctg gtttcaccgg aattgcgcaa attcgccggc ttggagaagc aagtgatgat
     5641 ggtcgggcag ggcagtcact tcgaaatctg gagccagagc acctgggaga aacagctcga
     5701 acaacttgca attggcggtg acaagctgcc cccgggaatg gagaacttcg cgctgtgagg
     5761 tcgagcggat caccacttgc gctcacatca gacggtactg cttgaggaag cgatcgaggc
     5821 cctgcgcatc aaggccgatg gcatctacgt agatggaacc ttcggccgcg gaggacacag
     5881 ccgggcgatt ttggcgcgcc tcggcagctc gggaaagctg atcgttatgg atcgcgatgc
     5941 catggcgatt gccgcggcaa cggaaatagc cgatccgcgc cttgccgtat ttcatgcgca
     6001 gttcggcgca ttgcgggaag tagttcgctt ggcgggtgtg gcgcatgtcg acggcgtgtt
     6061 gttcgacctc ggcatgtcct cggcgcaact tgacgaggcg gacaggggat tcagcttccg
     6121 cttcgacggg ccgctggaca tgcggatgga taggagccgg gggcaaaccg cggcgcaatg
     6181 gctggagcag tcttcggaac gggagatcgg ggaggtcatc aagcgctatg gcgaagaacg
     6241 gtttgctaaa cagattgcaa gagcgattgt tgcggctcga tcgcgcgggc ctgtcgaccg
     6301 cacacgccaa cttgccgaga tcgtggcaaa agccgttccc acgcgcgagc cacttcagga
     6361 cccggcgacg cgtaccttcc aagctatacg gattcacgtc aatcaggagc ttgaggagtt
     6421 ggcggtgatg ctgcctcggt gcctcgaggt cctcagtgac ggcggcagac tggtggtgat
     6481 cagtttccat tcgctcgaag accgtatcgt caagcagttc atgcgcacgc attcgctgcc
     6541 cggcgcactg ccggcgcgtc tgccgctgcg cgcccgcgaa ttgccgaaac cgtacctgag
     6601 gctcattggc aaaccggtct tcccctccga gtccgagatt gccgccaacc cgcgcgcccg
     6661 cagcgcggtg ctgcgggtcg cggaaaggat cgcggcgtga tcaagctcaa tctcgtgctg
     6721 tcgggaatcc tggtcgtctg cgcgctgggg ctggtgactt cgcagcacaa ggcacgaaaa
     6781 cttttcgtcg agttgcagaa gcagcaggaa gtcgccaagc agctcgaagt cgaatatgga
     6841 caattacacc tggaacaaag cacttgggca atgcattccc gcgtcgagaa gatcgcggcc
     6901 gacactctac gcatgcgtgt accgccggcg gcgcgcgtgc atctcgttcc tccccctccc
     6961 gcgccgtcca acccatgaga ccctccctga atcctgcgct atcggtgcgc ctgcctgtgt
     7021 ggcgggcgcg gctgctattg ctgctggttt tctgcgggct catgttgctg gcggcgcggg
     7081 ctttttattt gcaagggctg cataacgatt ttctgcagca gaaaggcgaa acccgctatg
     7141 cgcgcgtagt cgagatatcc gcgcatcgcg gcatggtgac cgatcgcaat ggcgaaccgc
     7201 tggcggtgag tacacctgtg gaatcggtgt gggcatctcc ggcggacgcc gacctcagca
     7261 ccgagcaaag agtgaagctc gcgcgcctgc tcggcacgga tgcaacggaa ctgaggagac
     7321 gcctttccga cagtgagcgc gagttcgtgt atctcaagcg acagttaccc cccgagcagg
     7381 cggcgaaagt cgtccagctc aatcttcccg gcgtgttcct gcagcgcgag taccgccgtt
     7441 actaccccgc agcggaagtg actgcgcacg tggtcggctt tacgggcgtg gacgacaacg
     7501 ggcaggaagg catcgagctg gcataccagg aatggcttgc gggaaagcgc ggcagccggc
     7561 gcgtgatcaa ggacaggcta gggcggatca tcgaggacat cgaaagcatc cgcgtgccgc
     7621 agcaggggcg cgatctggcg ctctccatcg atcagcgcat acagtatctt gccttcaggg
     7681 agatcaagag cgcggtcgcg gagcacgagg ccaaggcggg cagcatcgtg gtgctcgatg
     7741 cgacatcggg cgaagtactg gcgctggcga actggccgac ctacaacccg aacaaccgtg
     7801 acaccttgaa gatggcccgg tcgcgcaacc gcgcggtggt ggacctgttc gagcccggat
     7861 cgaccctgaa gccgttcacc gccgcggccg cattggagtc cggattggta ggtccgggca
     7921 gcgtcattga caccgagcat ggccgcttca ccatcggcaa gcgcacaatt cgcgacgtac
     7981 atcccgaggg ctttctcacc gtctcccagg tgatccagaa gtcatcgaac gtgggctcgg
     8041 cgaagatcgc gctggcgatg gcaccgcaga agctctgggc aatcttttcc gcagtcggct
     8101 tcggcaccca gaccagagtc ggatttcccg gagaagcaac ggggcggctg cgtgcctatc
     8161 aaagctggaa gccgattgag cacgccacca tgtcttatgg ccatggcgtt tccgtcagcc
     8221 tgttgcaact ggcgcgcgcc tactcggtat tcgccaccga cggcgaactg aagcaactga
     8281 ccttgattcg ccgcgaccag ccggtcgagg gcaagcgggt ggtatcgcgg cgcaccgcgc
     8341 aggcggtgcg caagatgctg gagatggtga cccagccggg aggaaccgcg acgcgcgccc
     8401 aggtcgccgg ttaccgagtt gccggcaaga ccggcactgc gcataaactc gacggtgcta
     8461 cgtacgcagc ggatcgctac gtttcctcgt tcgtcggttt cgcgcccgcc tcgagcccgc
     8521 gcctggtcat tgccgtgatg atcgacgagc caggtggaaa gtattactac ggcggagaag
     8581 tggccgcacc cgtattcagc aacgtgatgg cgggcgcatt gcggctgctc ggagtcacgc
     8641 ccgacgcgcc gcctgacaat gtgattcccc ccgggtctgc gccgctgatc ggggaggaag
     8701 tatgaacgcc gcagcgcgct cgccgcagcc gggccagcgc gccttcgacg tgcgtgacat
     8761 cgacgcgctc ggcgtgcccg tcaccggcct ggcggtggac agccgcaagg taaagcccgg
     8821 cgacctgttt ctcgcctacc cgggtgagca tgccgacggc cgccgcttca tcgaccaggc
     8881 aatcggcgcc ggtgccgccg cggtgttgtg ggacagccgc agcttcctgt ggaactccgc
     8941 atggaagctg cccaacctgg gcgtgcccaa gttgcgcacg cacctaggag aaatcgccag
     9001 cgacttttac gggcatccgt cccgacacct gtggatcgcc ggcgtaaccg gcaccaacgg
     9061 caagacctcc tgcgcgcact ggatcgcaca atcgctgtcc cgcctcgggc gcaagaccgc
     9121 ggtgatcgga accatcggca gcggctttcc cggggcgctt gaaccggcac tcaacacaac
     9181 tccggatgcc gtggcgctgc acgcccgcct tgcggatttc cgcatgcacc gcgccgaggc
     9241 ctgcgcgatg gaggtttctt cgcatggaat cgaacagggc cgggttggcg gcgtggagtt
     9301 cgacgtagcg ctattcacca acctgtcgcg tgaccatctc gactatcaca agaccatgga
     9361 ggactacgcg gccgccaagg ccaagctctt tcattggccg caactcaagc atgcgatcgt
     9421 gaatctggac gaccgcttcg gccagcaact tgccggttcg atcgatcgct cccgtgtcaa
     9481 tgtcctcggc tacggtttgg gcaggggcga gatcgccgga caccggctgg atctgtcaac
     9541 gcgcggtctc aagctggaga tcaccacgcc gtggggcgcc gccgaactcg aaagccccat
     9601 gatcggtggt ttcaacgcct ccaatcttct gggtgtgcta ggcgtgctga tcacttccga
     9661 tgtgccgctg caagacgccg ttcaggcttt gtcgcgggtc gagccggtgc cgggacggct
     9721 gcaaatggtg cgcgaacccg gtgcgccgct ggtggtggtg gattacgctc acaccccgga
     9781 cgcgctggag aaggtgctgg agaccctgcg cttcacgctt tcgaacgaag ggaaactgca
     9841 ttgcgttttc ggttgcggcg gtgaccgcga tccgggcaaa cgtccgctga tgggggaagt
     9901 ggcgacccgt ctggccgacc gcgtggtaat taccagcgac aaccccaggt ccgagaatcc
     9961 gcgcgcgatc atcgacgaga ttctcgccgg cgcgcacccg aaatacgcga tcgaagagga
    10021 tcgtgcagcg gcgatcctgc aggcgatcag cagcgccgcg ccgcaggacg tggtgctgct
    10081 cgccggcaaa ggccacgaaa cctatcagga aatcggtggc cggcggctgc ctttcgacga
    10141 tgccgaggtc gcgcgcgaaa tcctgcagcg attgccgcgg cggttgggcc atgcttgacc
    10201 tcgtcagcgc agcgcaggcg ctgggcgccc gtcgagtcgg cggcaatgcg gagttcgtgc
    10261 gcgtgaccac cgacagccgc gacattcgcc cgggcgatct gttcgtcggt atccgaggcg
    10321 aacgtttcga cggccaggag ttcgctgcgc aggcgctcgc agccggcgcg gtcggggcaa
    10381 tggtgagcga cggttcccgc aacgcggtcg caggcgccag gctgctggtg gttcccgata
    10441 cgcgcattgc gctggggcag ctcgcggcct actggcgcgc gcgctttgcg atgccgctga
    10501 ttgcgatcac cggcagtaac ggcaagacca cggtcaagga gatgcttgcc gcgatcctgc
    10561 gccagtcggc cggcgaaagc ggcgtgctcg ccacggtcgg caacttcaat aacgacatcg
    10621 gcatgccgct caccctgctg cagcttcgcg cccagcatcg ttacgccgta gtcgaaatgg
    10681 gcatgagcca cctgggcgag atcgcctacc tgtcgcggct ggccaagcct actgcggcac
    10741 tgatcaacaa cgccggaacc gcacatatcg gcgaactcgg ttccaccgag gccattgctc
    10801 gctccaaggg cgaaatattc gaaggcctgg atatggccgg gaccgcgatc atcaacagcg
    10861 acgacgcatt tgccgatttc tggcgcggcc ttgcgaaacc ccggcgcgtg gtggatttcg
    10921 ggctggacag gaaggccgcg gttttggcgc gctacgaact cgccgaggca ggcagtctgg
    10981 tcaccatcgt cacgccggag agcgagttcg tcgccacgct caacgtgccc ggtctccaca
    11041 acgtcaagaa cgccttggcg gcggcaactt cagcctatgc gctcgggatc gaatccagag
    11101 ccatcgccgc cggtatcgcg gcctatcgcg gcgtgaaggg caggctgcag cgcaagcgac
    11161 tcccatccgg cgctacgctg atcgacgata cctacaacgc caatccggaa tcgatgaagg
    11221 cggcaattgc cgttctggcg gctcatgccg gccgcaatgt tttcgtgatg ggcgacatgg
    11281 gcgaactcgg cgatgcggct tccgcgatgc atgcggcaat cggcgtattc gcaaagcgtg
    11341 ccggcgtgca gcgcctgttc gcgctaggag agctgagcgc ggaagcggta cgcggctttg
    11401 gcaaaggcgg tgcccacttc gcgaccatcg aagatcttat cgcggcaatt accggcgtgc
    11461 tcggccccaa tacgacattg ctgataaagg gctcgcgttt catgcggatg gagcgcgttg
    11521 tcgaggccct gggcgcagtg aacggggaac agactgcgaa aggaggcgcc tgatgctgct
    11581 gctgctcacc cagtggctgg ccgaggacgt acgtgccttc aacgtattca actacatcac
    11641 attgcgtgct gtgctggcca cgctgaccgc gttgctgatt tctttcgtcg tcggtccggc
    11701 gatgatacgc aagctcaccg cctacaagat cggccagtca gtgcgcgacg acggaccgaa
    11761 gacccatctg agcaaagccg gcacgccgac catgggtggc accttgattc tggtttcgat
    11821 caccatcacc acgttgttat gggcggacct gcgcaaccgc tacgtgtggg tggtgctggc
    11881 ggtaacgctg ggctttggca tggtcggctg ggtggacgac taccgcaagg tcgtgcatcg
    11941 cgaccccaaa gggctgtcgg cgcgcggcaa gttactctgg caatcgctga tcggtcttgc
    12001 cgcggccgtc tacctggcaa catcggccac gctgccggcg caaaccgagc tcatcgttcc
    12061 cttcttcaag caggtcgcgt atccgctggg gatcatcggt ttcgtggtga tgacctactt
    12121 cgtcatcgtc ggcaccagca atgcggtgaa cttgacggat ggactggacg ggctggcgat
    12181 cctgcccact gtgatggtcg gcagtgcgct cggcgtgttc gcctatgtcg caggcaacgc
    12241 ggtgttctcg aagtatctcg gatttccttc cattcccggc gccggcgaac tcacggtgat
    12301 ctgcgcggcg ctggccggag cagggctcgc cttcctctgg ttcaacgcgt acccggcgga
    12361 agtgttcatg ggcgacgttg gtgctctggc actcggcgcc gcgctcggca ccattgccgt
    12421 ggtggtcagg caggagatcg tgctgttcat catgggcggg gtgttcgtgg tcgaaacgtt
    12481 gtcggtgatg ctgcaggtcg cctcattcaa gctcacgggc aagcgcatct ttcgaatggc
    12541 gccgctgcat caccactacg aactaaaagg ctggaaagaa aatcaagtgg tcgtccgatt
    12601 ctggattatc accatgatgc tcgtgctcgt cggactttca acattgaaac tgcgatgaga
    12661 cgaccacttt atttcagtgg aggctcatgc acgcagtgcg tgatgccgga actaaaacca
    12721 ggttgtcgtc cgcttctgga tcatcacgat gatgctggta ctggtgggtt tatcaacgtt
    12781 gaaactgagg taacacatga ctagccgtct gcaattcgaa ttgttcatgc gggaaatgcg
    12841 cgcgcaagcc gccaacaaag ggccgaaaca actgccgctc gccaacttgc atcccgaaca
    12901 aaccgaggac gtcggcgcct tgcgtctgtc ccgaatcgag gctgcgatga aggagacgcg
    12961 tgccgctcga gctggaaaat aggcgcgtac tggtgctcgg tctgggctca agcggaatgt
    13021 cgatggcgcg ctggctggcg ggccgaggcg cacgcgtgca cgtcgccgac agccgccccg
    13081 tgccgccgaa tgccgaccag ttgcgcgagg agctgccgga catcgacctg cacacgggag
    13141 cgtttcgccc cgaaagtttc gccgacgcgg agctgattgc aatcagcccc ggcgtaccac
    13201 tcgccgaacc actggtgcaa gcggcgctgg cgcgaggctt ggaagtgatc ggcgatatcg
    13261 aattgtttgc ccgcgtacgc gacgaatttc ccggcgccaa agtcatcggg attaccggtt
    13321 cgaacggcaa gagcaccgta accgagatgg tcggcacgat gacgcgtgcc gccaggacca
    13381 ggacgctggt ggcaggaaac atcgggctgc cgattctgga agcgctttgg gaaatcgaag
    13441 ccggacggcg gcggcgccct gacgtgttcg tgctcgaact gtccagtttt caactggaga
    13501 ccacggagaa cctcaatgtc gacgccggca cggtcctgaa cctgagcgag gaccacctgg
    13561 accgctacgc gggcattcgc gaatacgcac aggcgaaggc gcgcattttt ttcggcaacg
    13621 gcgctcaggt gataaaccgc gaagacgcgt gggcgcgcga aatggccgcg tcgggcaggc
    13681 gcgtattcac attcggactc gacgaaccgc gcgaagaggg tgcgtgggga ttgcgcaacc
    13741 tcgacggaga actctggctc gcggaagggc gggccaacct gatgaaagtt tccgaattga
    13801 aagtgacggg cctgcataac gccgcgaacg cgctggcggc gctggcgttg tgccgagcgg
    13861 tcgggtttcc ctatgaagcg ctgctcgatg cgctcttcag cttcagcggc ctgccgcatc
    13921 gcgttcaacg gattgtcgat atcaacggcg tggctttctt cgacgattcc aagggtacca
    13981 acgtgggcgc gacggtcgcc gcgctcgagg ccatgggtcg gaaggttgtg ctcatcgccg
    14041 gtggcgacgg caagggccag gatttctcgc cactacggga tgcggttgcg cgtcacgccc
    14101 gcgcggtggt gctgatcggg cgcgaccgcg acaggatcgc cgcggcgatc gagtccacgg
    14161 gagtggtgct ggtgcgcgcc agcgacatgc acgaggcggt gcgcgccacc cttgccctgg
    14221 cgcacgccgg ggatgccgtg ctgctgtcgc cggcgtgcgc cagcttcgac atgtttcgcg
    14281 actacgagca ccgcgcgcag gtgtttgtcg acgaggtcag ggccttggaa ggagcgcaat
    14341 gaacggcgtg ctgggccacg tggcgaaccc gccggcgccg atagccgaac tcgactacgc
    14401 ggtcgtcagt ctggcggcgc tactgctcgg aaccgggttg gtgatggtgt actcctcgtc
    14461 catcgccatt gccgaggcag gccactacgc cggcaaccag ccggcgtact tcctcattcg
    14521 ccacagcgtg ttcgttttcg tcggagtaat ggccggcttc atctgctttc agttcccgac
    14581 acgcgtatgg cagcaagccg caccctatct cttcgtgctt ggtgtactcc tgctgttgct
    14641 ggtgttgatt cccggcatcg gcaaggaggt caacggcagc cagcgctggc tttcgctggg
    14701 gatgttcaac ttgcagccct ctgaactgat gaaactgctg gtgctgttgt acgccgccga
    14761 ttacacggtg cgcaaggcgg cattgatgca cagtttccgc aagggcttcc tgccgatgct
    14821 gctggtaatg ctgctggtcg gaggactgtt gctgcgcgaa ccggacttcg gcgcgttcgt
    14881 ggtcatcacg gtgatcgcga tgggtgttct gttcctggga gggatgaact ggaagctgtt
    14941 cgctgtcctg atcatgcttc tgatcatcgg cttcgtgctg ttgatctggt cttcgccgta
    15001 ccgcctgcag cgcgtcatgg gcttcatgga tccctgggcc gacccgtacg gcaagggcta
    15061 tcagttgtcg cacgcgctga tcgccttcgg tcgcggcgaa tggttcggcg tgggcctggg
    15121 tgccagcgtc gagaaactgt tctacctgcc ggaagcgcat accgactttc tgctggcggt
    15181 gatcgccgaa gaactgggat ttgcaggcgt gatcgccgtg atcgcgctgt tcggctggct
    15241 gacctggcgt gcctttgcga tcggcaatca ggcagcgcgg ctggagcgct acttctcggc
    15301 gttggttgcg cagggtgtcg gactgtggat cggcgcgcag gcggccatca acatgggcgt
    15361 gaacatgggc gtgctgccga ccaaggggct caccttgccg ctgctctcgt tcggcggcag
    15421 cggcatcgtc gccaactgct gtgcgctggc gatcctgctc agggtcgatt acgagaaccg
    15481 ccagatgatg aaaggccttt cgttgtgaac gggatgagcg attcaggcaa cgcgcgaacc
    15541 atcctggtga tggccggcgg gaccggtggc cacatctatc cagctcttgc ggtagccgat
    15601 ttcctgcgcc agcagggctg gaaagtggtg tggctgggcg cgcgcagcgg gatggaagcc
    15661 atgctggtgc cgcagcacgg ctatgcggtc gagtggatcc ggttttccgg cctgcgcggc
    15721 aaaggcatcg tgcgtctggc tctgctgccg ttgaatctcc tgatcgcgtt ctggcaaagc
    15781 gcccgcgcca tcttcagggt gcgtcccgac gtggtgctgg gcatgggcgg ctacatcgcc
    15841 tttcccggag gaatgatggc tgcgcttctc gaccggccgc tggtactgca cgaacagaac
    15901 tcgatcgccg gccttgccaa caaggtgctg gcacaggttg cagaccgcat cctggtggcg
    15961 tttccgggcg cgctgaagaa gtccgcgtgg acgggcaatc cggtgcgccg cgaaatcgcc
    16021 gaactgccgg cgcccgagca gcgattccgc gaccgcagcg gcaatctcaa agtgctggtt
    16081 gtgggtggca gtctcggtgc gcagatattg aatgacacgg tgccgcgggc gctggcgctg
    16141 attccgcaga cccgacggcc agacgtcacg catcaggccg gcgaaaagca catcaccacg
    16201 ctggagaaga actatcgtga cgcgggcgtg aaggcgacct cgcttgcttt cattcacgac
    16261 atggcaggtg cctacgccga tgccgatctg gtgatctgcc gcgcaggcgc cttgacggtg
    16321 gcggaactcg ccgccgcggg cgtggccagc attctcgtgc cattcccgca cgcggtggac
    16381 gaccaccaga cccacaacgc ccgtttcctg tccgacaaat cggccgcggt gctgctgccg
    16441 caaaaagaac tgacgccacg caagcttgcc gatctgctgc tcggcttcac ccgcgaaaga
    16501 atcctggaaa tggcaaagct tgcgcgttcg ctcggaaaac cggacgccac gcgcgtggtc
    16561 gccgagcatt gcatggccgc ggccggctag aaaaacatga accacagaga cgcagaggca
    16621 cagagagtat tcgagtctga acagaaccag cgtgcggaag cgcagccctt atggcgcctg
    16681 ccgccgaatt gcacggcgat gaataaggaa tagatgaagc acaaggtcaa acacgtgcat
    16741 ttcgttggca tcggcggcgc cgggatgagc ggcatcgccg aggtgatgct caatttgggc
    16801 ttcgaggtga gcggttccga cattgccgac agcgccgcga tccagcggct caaggcgctg
    16861 ggcgcgaaag tgcacatcgg ccacgccgat gccaatgtca gcggagcaaa cgctgtcgtc
    16921 acctccaccg ccgtcaagcc ggacaacccg gaagtggctg ccgcgcgcgc cagacgtatc
    16981 ccggtagtgc cacgcgcgat gatgctggcg gaactgatgc ggctcaagca gggcatcgcg
    17041 gtcgccggca cgcacggcaa gaccaccacc accagcctgg tcgcgagcgt gcttgcggag
    17101 gcggacatgg atccgacttt cgtgatcggc ggccggctcg aagcggccgg ttcccacgcc
    17161 aaactcggca gcggcgattt catcgtggtg gaggcggacg agtcagacgc ttcttttctt
    17221 tacctgcagc cggtgctggc agtggtcacc aatatcgacg cggaccacat ggagacctac
    17281 ggacatgact tcagcaagct gcgccaggcc ttcctcgagt tcatcgagca cctgcccttt
    17341 tacgggatgg cggtgctgtg catcgatgac gccaacgtcc gcagcctgat gccgctcatc
    17401 acccggccgg tcaccaccta cggcctggcc gacggcgcgc agattcgcgc ggtggacgtg
    17461 gaggcggcgg cgggtcagat gcggttccgc gtggtgcgca agatcggctt caacgggcac
    17521 gcgcaggcgg cgcccgacct gcgaatcacg ctcaatctgc cgggcctgca caacgtgcag
    17581 aacgcactcg ccgcaatcgc ggtgggtatg gagatcggtg cgcacgacgc tgcgatcgtc
    17641 aaggcgctgg cggaatttcg cggcgtcggg cggcgcttcc aacgttacgg cgacgtggcg
    17701 ctgcgcaacg gcggcagctt cacgttgatc gacgactacg ggcatcaccc ggtggaaatg
    17761 gccgcgacca tcgccgcagc gcgcggcgcc tttcccggcc ggcgtctgat gctggcattt
    17821 cagccgcatc gttacaccag gacgcgtgac tgcttcgagg acttcgtgaa agtgctgtcc
    17881 tcggtcgacg cgctcctgct taccgaggtc tatccggccg gcgaggcgcc gatcgttgcc
    17941 gcagacggcc gggcgctggc gcgtgcggtg cgcgtcgccg gaaaggtcga gccggtattc
    18001 gtcgagcatg tgaacgaact gggggcagca atcctggcgt ccgtccagcc cggggacgtg
    18061 gtgatcacga tgggcgccgg ttccataggc ggcgtgccgg gaaaaatggc aaggatgaag
    18121 gatgaagcta atgccgacta ccgccatcgc tgacacatat ggatatgtct gaacaggagc
    18181 gattagggac acccgcgctt cgcggcgaac tgcgacgcga cgagccgatg cgcaagcgca
    18241 ccagctggcg cgccggcggg accgcggatc gcgcttacct gccggccgat ctcgacgacc
    18301 tgtgcgtgtt cctgcggggc ttgccgggcg cggagccggt tttcttcgcc ggtctgggca
    18361 gcaacttgct cgtgcgggac ggcggcatcc gcggcacggt ggtgttcacg catgcagcgc
    18421 tgcggcgatt cttcctcgac gagggggccg gcgcggcgga gatctgcgcc gaggccggag
    18481 tggcctgccc gaaagtggcc cgcttcgcgg ccgtccacaa ccttgaaggc gcggagtttc
    18541 tcgccggcat tcccggcacc gtcggcggag cgctggcgat gaatgccggc tgttacggcg
    18601 cggagacctg ggatgtggta gcgagggtgc gcacggtcga tcgctgcgga cgattgcaga
    18661 tccgttcgcc aaacgaatac tccatcggtt accgccacgt cattccgtta caggcgcagg
    18721 acgaattttt cgttgcggcc tggttccgtc tccgtcacgg cgacggtggc cgcggccgcc
    18781 agacgatcag gaatctcctg tcgcgccgca tcgccagcca gccgctcgat gaacccaacg
    18841 cgggctcggt attccgcaac ccgcccggcg atcatgccgc gcggctgatc gaatcctgcg
    18901 gactcaaggg aaaacgcatc ggcgcagcgg aggtatcgct caaacacgcg aacttcatcg
    18961 tcaacaaggg cgatgccact gcgaaggaca tcgaagaact gatcaccctg gttcaggaaa
    19021 ccgtgaagcg caaaaccggc gtcgagctgg aacgcgaagt gcagatagtc ggtgaggcga
    19081 gaggcgaggg gcgaaaggcg tgagggcaaa gaatttcgga aaggtggcgg tgcttcttgg
    19141 gggtcgctcg gccgagcggg agatttctct catgagcgga aacgccgtgc ttgcggcgct
    19201 cgctcgcagg ggcgtggacg cgtatgcctt cgatacccgc gaccgtgatc tgcacgagct
    19261 gcgcagccag gaaatcgacc gcgcgttcat cgcactgcat ggacgctatg gggaagacgg
    19321 cacggtccag ggcgcgctcg agctgatggg catcccgtac accggcagcg gcgtgatggc
    19381 gagcagcctg gcaatggaca agtggcgcac caagctggtc tggatcgccg ccggcattcc
    19441 tactccgcgg ttcgtgatgc tggaggcggg ttccgacttc aaggcggtgg cgaatgaact
    19501 cgggctgccg ttggtcgtga agccttcgcg cgagggctcg accatcggcc tgaccaaggt
    19561 caccggcgcc cagagcatgc aggcggcgta tctaaaggca gcggagtacg acgacatggt
    19621 gatggccgag gaattcatcg cgggcgcgga agtgacggcc tcgatcctga gagagcagac
    19681 gttgccgttg atccgcatcg aggcgccgga cagccgctac gactaccagg cgaagtattt
    19741 ctccgacgag acccgctact tctgtccgtc cggattgcct cccgcacagg agcgccgcat
    19801 tcaggacctc gctctcaagg catcccgcat cctcgactgc cgaggctggg ggagggtgga
    19861 cgtgatgctc gacgccaaag gcgagcctta cctgttggag gtgaacacgg tgccgggaat
    19921 gactggtcac agcctggtgc cgatggcggc aaagggagcg ggcatcccgt tcgacgacct
    19981 ggtcgtacgg attctggagg acgcgcatgt gggataacgc cgccgtgctg aaccgggcag
    20041 ccaatttact ttacggcgcc gccgcgctgc tgattgttta cggctgcatc cattacgtag
    20101 tgcatctgcc ggtgttcccg ttgcgcgaaa ttcgcgtcat cggcgacgtc ggccacgtga
    20161 ccaacgaaca ggtcgcggcg gtgacagcgc gcgaactgcg gggcaatttc ttcaccgtcg
    20221 atctggcaca ggcgcgcgaa gcgttcgaaa aactgccttg ggtgcgcacg gtcaacgtgc
    20281 gcaggcaatg gccggatcgc ctggaggtcg cggtggaaga gcaccagccg ctggcgcgct
    20341 ggggctcgac cgcgctggtg aacgcgcacg gggaagtgtt cgaagccgcg atcagcagca
    20401 agctgccggt gtttttcgga ccggaaggca cggccgtgga agtcgtggcc cgctatgccg
    20461 agttcgatcg tttgctcgcg ccgatcggaa ggaaagtggt gctgatcacg ctttccgcgc
    20521 ggcgtgcatg gcaactgcgt ctggacgacg gaacggtgct gcagctcgga cgcgaaaacc
    20581 tggaggcgcg actcgcaggt ttcgtatcgg cctacccgcg caccgtggca cggctcccgc
    20641 agccgccttc gcacgtggac ctgcgctaca gcaacggttt tgccgtgcgc accccgggac
    20701 tgaaatggga cgccaggaaa gcctgatgaa acggtgatat gagaccaatg gcactgagtg
    20761 gaaggaagga aagcaagaat ctgatcgtgg ggctggacat cggcacatcg aagatcgccg
    20821 ccatcgtcgc ggagatgaag ccggaaggcg gcttcgaggt gattggcatg ggcagttcgc
    20881 cttcgcgcgg cttgaagaag ggtgtggtgg tgaacatcga gtccactgtc aacgcgattc
    20941 agcgcgcgct ggaagaggcg gagctgatgg ccgactgcaa aattcgcgag gtctggaccg
    21001 gcattgccgg cagccatatc aggagtttca actcccacgg catggtggcg atcaaggacc
    21061 gcgaggtggc gcagacggac gtggaccgcg tggtcgagac cgcaaaggcg atcccgatcc
    21121 cgaccgatca gcagatcctg cacgtactca accaggagtt cgtgatcgac ggccaggagg
    21181 acgtgcgcga gccgttgggc atgagcggtg tgcggctgga ggtgaaggtg cacatcgtca
    21241 ccggtgcggt ctcggcggca caaaacatca tgaaatgcgt gcgccgctgc gggctcgagg
    21301 tcaacgacct gatcctgcag ccgctcgcgt cggccaccgc ggtgttgtcc gaggatgagc
    21361 gcgacctggg cgtatgtctg gtggacatcg gtggcggcac ctccgacatc gcggtgttca
    21421 cccagggcgc gatccgtcac accgcggtga tcccgattgc gggtgaccag atcaccaacg
    21481 acatcgcgat ggcgctgcgc acccctacca aggaggctga ggaaatcaag cgccgctacg
    21541 gctgcgcact gagagaactg gcgaatgcgc aggagatggt ggaagtaccc ggcgtgggcg
    21601 accgggaacc caggaagatg tcgcggcaga cgctggccga ggtgatcgaa ccgcgggtgg
    21661 aggaactcta ttcgctggtg caggcggaat tgcgccgcag cggcttcgag gagttgctct
    21721 catcgggaat cgtgatcacc gggggaagca gctcgatgca gggcatggtg gaactgggcg
    21781 aggaggtgtt ccacatgccg gtgcggctgg ggctgccggg ctatgccggc ggtctgtcgg
    21841 aagtgattcg taacccgcgc tactcgaccg gcgtgggttt gttgatggcc gggctgaacc
    21901 aacaccaccg ccatcagata gagaagatgc agagcggctc gttcaagcag attttcgggc
    21961 gcatgaagaa atggttcgag acgaattttt aatcggcagg attcaaaggg tgagtctggt
    22021 ttcggttttt cgtgatcatt cacagaggag atgagaaatg tttgaaatcg tggatacgca
    22081 accccaggaa gcggtgatca aggtggtagg cgtgggcggc tgcggtggca acgcggtcga
    22141 tcacatgatc aatgaaggcg tggaaggcgt cgagttcatc tgtgccaaca ccgatgcaca
    22201 ggcgctcaag cgcaacaagg cgaagaccct gctgcagctc gggccgaatg tgaccaaggg
    22261 cctgggcgcc ggcgccaatc ccgacatcgg ccgcgaagcg gcgatggaag accgcgagcg
    22321 catcatcgag ctgatcgaag gcgcggacat gttgttcctg accgcgggca tggggggcgg
    22381 aaccggtacc ggtgcggcgc ccatcgtcgc tgaagcggcg cgcgaactcg gaattctcac
    22441 ggtcgcggtg gtaaccaagc catttgcatt cgaaggcaag cgccagaaga tcgcgcaaca
    22501 ggggctggac gagctgtgcc ggcacgtcga ttcgctgatc gtgatcccaa acgacaaatt
    22561 gatgcaggtt ctcggcgagg atgtgagcat gctcgatgcg ttcaaggcag cgaatacggt
    22621 gttgcacggc gcggtggccg gtattgccga ggtgatcaag tgccctggac tggtgaacgt
    22681 cgatttcgcc gacgttcgca ccgtgatgtc cgaaatgggc atggcgatga tgggttcggc
    22741 aatggccgcg ggtgcggacc gcgcgcactg tgccgcggag caggcggtgg caagcccgct
    22801 gctcgaggac gtgaatctcg cgggtgcgcg cggcgtgttg gttaacatca ctgccagcgg
    22861 caccctgaag atgaaggaag tgcacgaagt gatgaacacc atcaagggtt tcaccgccga
    22921 ggatgccacg gttatcgtcg gtaccgtgat cgacgaagcc atgaatgacc ggctgcgcgt
    22981 taccatggtg gcgaccggcc tgggcggggc cgctgcagtc aaccgccacc atcagaagcc
    23041 gttgacgatg gtaaaaaccg gtaccgatgc cggaccgctg gcggtgaact acggggagct
    23101 cgatgctccc gccgtgatgc gccggcgttc gcgcgatgcg gcggtggagg cgatgaagca
    23161 atcgggcgtg gacatgctcg acatcccggc gtttcttcgc aaacaggcgg actagaatcc
    23221 ggcgcagatg aagcgccttt gcaacgcagc aatcgttgct gccgtgctgg tacttcgtgc
    23281 gttgctatgg tagtctaatc aaccgtttat ctttaacagc agacgacggc ttacgaggcc
    23341 gcaccgcgag aagcatcgac atgatcaagc aacgcacgtt gaagaacata gttcgtgcca
    23401 ccgggatcgg cctgcatacc ggtgaaaaag tctaccttac gctgcgcccc gctgctccca
    23461 acaccggtat cgtgttccaa cgcgtggacc tcgagccagt ggtggacatc aaggccgatc
    23521 cttacgccgt aggggacact cgcctgtcgt cctgcctgga gcgggccggt gcccgggtcc
    23581 agaccgtcga gcatctgatg tcggcgctcg caggcctggg gatcgacaac gtctatgtcg
    23641 atctgaccgc cggcgaagtg ccgatcatgg acggctccgc ctctccgttc gtgttcctgc
    23701 tgcagtcagc ggggatcgac gaacaggccg ccgccaagaa attcatccgc atcaagaagc
    23761 cggtcgaatt gaagcagggc gacaagtggg tccggttcga gccttacaac ggtttccgcg
    23821 tcgatttcag catcgatttt aaacaccccg tgttcgccag ttccaacaag cgcgtggtcg
    23881 tggacttcga acacacttcg tatataaagg aagtcagccg cgcccggact ttcggattca
    23941 tgcaggacgt cgagaatatg cgggcacagg ggctggctct gggtggcagc ctcgacaacg
    24001 ccatcgtcat ggacgaatac cgggtgctga atgcggacgg cctgcgctac gaggacgaat
    24061 tcgtcaagca taaggccctg gacgcgatcg gcgacctcta tttgctggga catccgttga
    24121 tcggagccta tacggcctat aagtccggcc atgcgctgaa caacgggctg ttgcgccggc
    24181 tgatggagga cgccacggca tgggagttcg tcagcttcct tagtcctgaa gaggcgcccg
    24241 ctttcctgcg gctgcagatt caggctgcct gattttgcgg ccgacctggc gctccggttg
    24301 gttttttcgg ccgtattgct gcggccagcc ggataaccga ataccatgtt gctccttaga
    24361 atcctcggtg cgctgctgtt gaataccttg ggcgaaacct tggtcggata tgttttgacc
    24421 aaggatctcg ccagtggctg cgcttcaccg cccgcgcgct tttggttgtg ttcgttatag
    24481 ctctgatttt tatggcttta tatgctattg agcggttagt cgtgacgatc tggcaagggc
    24541 gtcttgcgac gggcgaggcg ctccagggca gatttgagcg gtgagacgtc cagcgtcgca
    24601 gacaattcgg aaatcgcctg gcgggctgtc tggcttaaag aaattcgttt ttgtagcaaa
    24661 gggttatggc ggatccttac ttgcacttgt acgcgaattg ctgtaacctc atgtctccgt
    24721 tgtcggaaaa agaccaggat tcgcggcgcg aggtgtttca gtttggcggc agcggcgccg
    24781 ttgtcagcga acagaaccag ttcttcggca gctgaaagag ccacggtggt cgaagaggcc
    24841 aggctgtcgg gcaacacttc agtcagtagg tcttgcaggg caataagccg ccgaacctgg
    24901 cgattgaggc tctgcagttg cggaagtctg tcgatgagca gtccaatgcg ttcgctggtc
    24961 acggctggtt gaggccttga aggcacagaa ggggaacgga tgaatattat tctggtttcc
    25021 aacaaattcg cgcaggcgcg tagcttcgcc gtcaacacgc cccaggtcat cttcctcggg
    25081 ctcttgctcg gccttctggt ggtgtcgctg gccgtcctga tgaattactt ctcgctgcgt
    25141 tatgcagtca ccaaccaaag cccgtatctg cagtccctgc tggaggcggt acaagccgaa
    25201 caaacccgca agacccagtc ctacctgcgc gaaagcctga ataccatggc cgcacggctg
    25261 ggggaaatgc aggcgaagct gcagcggctc gacacgctgg gcgagcgcct caccaaactt
    25321 gccggcatca agccgcagga attcatgttc gaccaggcgc cagcgcgcgg cggcgcggtg
    25381 tccaccatcc cgtcgaagga actctcgatg ggcgagcttg gcgaaaatct ggaccggttg
    25441 gcgcaacagg tcgatgaccg atccgataag ctcggtgtcc ttgaatcgat gctgacgctg
    25501 gtgcacgcga aacagcagtt gctgcctacc cagcttccgg tgaacaccgg ctggtactcg
    25561 tcgaacttcg gctggcgcat cgacccattc accggatgga atgccttcca cgaaggaatg
    25621 gacttcatgg cgcaggaagg cgaggcggtc acagctgctg ccggcggcgt cgtggtgttt
    25681 tcggacccgc accctcagta tggtaatatg atcgagatcg atcatggcaa cggtttggtc
    25741 acgcgctacg ctcatgcgtc caagttgatt gcaaaagtcg gagacgtcgt tctgcgcggc
    25801 cagaagattg ccgaggtcgg caataccggc cgatcgaccg gaacgcatct gcacttcgaa
    25861 gtgcgtcaat ggggagcccc acagaatccc gcgcgcttcc ttcaagtgcc gggctgaacg
    25921 aactgctaga cgatttcagc ggaggggtgg gggcaggcgt ccccacccct cttgctttga
    25981 ggaagctctg ttgaatgttc acgaaccctc tcaagaaaat attcggtagc cgcaacgaca
    26041 ggctgctgcg caagtatgcg cactccgtgc aggcgatcaa tgcgctcgaa cccggattgc
    26101 gcgaggtgag cgatgccgaa cttcgcgcgc gcaccgaggt attcaagcaa cgcgtgcaga
    26161 acggcgaaac gctcgacgat ctcctcgtgg aagtgtttgc gacagtgcgc gaagccagcc
    26221 ggcgcgtgct caacatgcgc cactttgatg tgcagttgct cggaggcatc gctcttcaca
    26281 acggcaagat cgcggagatg cgcaccggcg aaggcaagac gctggtggca accctgccgg
    26341 cctatctcaa cgcgctctcg ggccgcggcg ttcacgtcgt cacggtcaac gactatctgg
    26401 ccaaccgcga tgccgagtgg atggggcgcg tgtaccgctt ccttggcttg agcgtcggcg
    26461 tcaatctgtc gcagatggag cacgagctga agcagcaggc ttatgctgcc gatatcacct
    26521 acggcaccaa cagcgagttc ggcttcgact atctgcgtga caacatggtg tttcaggcca
    26581 gcgagcgggt gcagaggggt ttgacctatg ggattgtcga tgaggtcgac tcgatcctga
    26641 tcgatgaagc gcgaacaccg ctcatcattt ccggccaggc ggacgacagc accgaccttt
    26701 attaccggat gaatgagatc gttcccacgc ttgcccaggt caaggaggag aacggccccg
    26761 gcgacttcac cgtggacgaa aaggcgcacc aggtgctgtt gaccgaggcc ggtcacgagc
    26821 gcgccgaaga cctgttgacc aaggccggcc tgttgccgga gaacggcagc ctgtacgacg
    26881 ccgccaacat cgcactcatg caccacctgt acgccggact gcgggcgcac tcgctgttcc
    26941 atcgcgacca gcactacgtg gcgcagaacg acgaaatcat catcgtcgat gaattcacgg
    27001 gacgactgac gcccgggcgg cgctggtccg atggcctgca ccaggcgatc gaggccaagg
    27061 agggcgtgcg catccagcgt gagaaccaga cgctcgcttc gatcacctac cagaattatt
    27121 tccggatgta caagaagttg gcgggcatga ccggcactgc cgacaccgag gccgaagagt
    27181 tcgc
//
LOCUS       DASQOP010000039         3049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1424101, whole genome shotgun sequence.
ACCESSION   DASQOP010000039 DASQOP010000000
VERSION     DASQOP010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1424101"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="VE030_01515"
     CDS             <1..762
                     /locus_tag="VE030_01515"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZF22125.1"
                     /translation="FKGAEFQVPYEHPPAELAARLKAHGLRMVLFDAPMGNWDAGDRG
                     LAAVPGREKEFRDSLVRVVEYGHALGCDTVHTMAGVIGPGLDYQAAERTYVENLRYAA
                     GFLEKHGIAVVIEPINKKMGLVKNGPSYTTQGMHGYFLNHTEHARRILEQVGSRNLYL
                     HLDVYHMQMLEGHLAETIEANIGLTRHFQIAGVPGRHEPTVGEINYPYLFDLLDSLGY
                     QGWIGCEYRPKGNTWEGLDWAARYGIRAPEALSAK"
     gene            complement(936..1085)
                     /locus_tag="VE030_01520"
     CDS             complement(936..1085)
                     /locus_tag="VE030_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22126.1"
                     /translation="MDTLLETFLRLVRNSRREPAQLDTDATVSIETRLLDLASKDHGT
                     FPYLP"
     gene            1223..2155
                     /locus_tag="VE030_01525"
     CDS             1223..2155
                     /locus_tag="VE030_01525"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZF22127.1"
                     /translation="MRLYVRIVETGSFSAVARELGTIQPTVSKQLTALEEHLGVRLLN
                     RTTRQLSPTEAGRKYYDRCRHILDEVTDLEGSLSDLQNRTTGLLRVNAPVAFGQLHML
                     PLVFRFRQKYPGLAVDLTLNDRFVDLVQEGVDVAIRFGDLSDSQVVARRVGGVSRVCV
                     ASPDYLRARGTPHSPADLSAHNCITYNYLFSNEWQFEGPQGPLSVRVKGDFRANSAIT
                     IRAAALEGIGIAHMPVVFVQEDIDKRRLTQVLSDYGPPPVDIHAVYPSARFLSSKVRL
                     FLDFVRDEFLAIPALQVRTDRPKRVPRIRAKVAS"
     gene            2180..2680
                     /locus_tag="VE030_01530"
     CDS             2180..2680
                     /locus_tag="VE030_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010116380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZF22128.1"
                     /translation="MNEKAAPTFHIRPATPEDVPLVLAFVRELAEYERLSHLVSATEE
                     RLRESLFGERPGAEVLLGFAGDTPAGFAVYFHNFSTFLGVRGLWLEDLYVRPALRRHG
                     YGRTLLLHVARIARSRGCGRLEWAALDWNEPALRFYKSLGAVAQDDWTIFRVTGPALE
                     KLADMH"
BASE COUNT          607 a          988 c          916 g          538 t
ORIGIN      
        1 ttcaaaggcg cggagttcca ggtgccttac gaacatccac ccgccgagct cgcggcacgg
       61 ctgaaggcgc atggcctgag gatggtgctg ttcgacgcgc cgatggggaa ctgggatgcc
      121 ggcgaccgcg gtttggccgc agtgccgggc cgggaaaagg aattccgcga cagcctcgtg
      181 cgcgtcgtcg agtacggcca cgcgctgggt tgcgacaccg tccacaccat ggccggggtg
      241 atcgggcccg gcctggacta tcaggccgcc gaacgcactt acgtggagaa cctgcgctac
      301 gccgccggct tcctggaaaa gcacggcatc gcagtcgtga tcgagccgat caacaagaaa
      361 atgggcctgg tcaagaacgg cccgagctac accacgcaag gcatgcacgg ctactttctc
      421 aatcacaccg aacacgcgcg gcggatcctg gagcaggtcg gaagccgcaa tctgtacctg
      481 cacctcgacg tctatcacat gcagatgctc gagggacacc tggccgaaac tatcgaggcg
      541 aacatcggcc tcacgcgcca tttccagatc gccggggtgc cgggccgcca tgaaccgacg
      601 gtgggagaga tcaactatcc ctacctcttc gacctgctgg attcactcgg ctaccagggc
      661 tggatcggct gcgagtaccg tcccaaggga aacacctggg aagggttgga ctgggcggcg
      721 cggtacggga ttcgcgcccc agaggccctg tcggcaaagt aagtctgccg ataccggcag
      781 gcaaaaaaag aggggcgctt ggcgcccctc taaaaccacg tagctcaagg aggaggagag
      841 agttacgtgg gggaggaaat cgtttccctg tccacactgt ccgggctttc ccaggcccgg
      901 ccatcccgcc caatccgctg attcagcagc ctcgcttacg gcaggtaagg gaaagtgccg
      961 tgatccttgg acgccaggtc caatagcctc gtctcgatgc tcacggttgc atccgtatcc
     1021 agctgcgccg gctcccggcg cgaattcctg accagtcgta aaaacgtttc aagcagcgta
     1081 tccatttcga acctccacca attgggctat tgccatgatt caaatatagg cttatgcata
     1141 atctattaca atagacacgg ttcgaatagg atttatgcga attaagtgct aaattgaatt
     1201 taagatggat cgattgtacc aaatgcgcct ttacgtccgc atcgtggaga ccggaagctt
     1261 ctccgcggtg gcccgggaac tgggcaccat ccagcccacg gtcagcaagc aactcactgc
     1321 tttggaagaa catttgggtg tccggctgct caaccgaacc acccggcagt tgagtccgac
     1381 cgaagccggc cgcaagtact acgaccgctg ccggcacatc ctcgacgagg tcaccgacct
     1441 cgaaggcagc ctctctgatc tgcagaaccg caccaccggg ctgttgcggg tcaacgctcc
     1501 tgtggccttc ggccagctcc acatgcttcc cctggtattc cgcttccggc aaaagtaccc
     1561 ggggctggcg gtggacctga ccctcaacga ccgcttcgtg gacctggtgc aggaaggggt
     1621 ggacgtcgcc atccgtttcg gcgacctctc cgactcgcag gtcgtggccc gtcgcgtggg
     1681 cggggtcagc cgggtgtgcg tcgcctctcc ggactacctg cgcgcgcgcg gcacgccgca
     1741 ttcaccggcc gatctgtccg cgcacaactg catcacctac aactacctgt tcagcaacga
     1801 gtggcagttc gaagggccgc aggggccgct gtcggtgcgg gtcaagggag acttccgcgc
     1861 caacagcgcc atcaccatcc gcgccgccgc gctcgaaggc atcggcatcg cccacatgcc
     1921 ggtggtgttc gtgcaggagg acatcgacaa acgccggctc acgcaggtgc tgtccgacta
     1981 cgggccgcct ccggtggaca tccatgccgt gtatccgtcg gcgcgtttcc tgtcgagcaa
     2041 ggtgcgcctg ttcctcgatt tcgtgcgcga cgaattcctg gccatccccg cgttgcaggt
     2101 caggaccgac aggccaaagc gggtgccacg catccgcgcg aaagtcgcat cctgaaatca
     2161 cgctgaccgg agtcgcgaca tgaacgaaaa agcggcaccc acattccata tccgccccgc
     2221 cacgccggaa gacgttccgc tggtgctcgc attcgtcagg gaactcgccg agtacgaacg
     2281 tctcagccac ctggtctcag cgaccgaaga gcgcttgcgc gaatcgctgt tcggggagcg
     2341 tccgggcgcg gaagtcctgc tcggtttcgc gggcgacact ccggccggct tcgcggtgta
     2401 cttccacaat ttctcaacat ttctgggcgt caggggcctg tggctggaag atctttacgt
     2461 gcgtcctgcg ttgcgccggc acgggtacgg gcgcacgttg ctgctgcacg tcgcgcgcat
     2521 cgcgcgcagc cgcggctgcg ggcgcctcga gtgggcggct ctggactgga acgaaccggc
     2581 gctccgtttc tacaaatcgc tgggcgcggt ggcccaggac gactggacca tcttccgcgt
     2641 gaccggacct gcgctcgaga aactcgccga catgcactga aagacacatg aaggatcctg
     2701 tgggacgcac tacaatacgc gccgtgagtc gtaccgcagc ggaccgcaac acctcccgga
     2761 tggccaggcc ggccctccac ggattcctgc cctccggact tttcccgcac tctgcatcgg
     2821 ccgccggtgc gggcggcctg tttgcattga agaaacgcgc gacctgaaaa ccgacatggc
     2881 aaggaaaccc aacgcctaca ccgcagaaga catcgagatt ctcgaggacc tatcgccgat
     2941 cctgcaccgg ccgggtatgt acaccgaccc gtcgaatccg aaccacgcgc tggtcgagat
     3001 catcgacaat gccaccgacg aaggcctcgc gggattcgca aaaaatgtc
//
LOCUS       DASQOP010000040         7750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1626499, whole genome shotgun sequence.
ACCESSION   DASQOP010000040 DASQOP010000000
VERSION     DASQOP010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7750
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1626499"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1720)
                     /locus_tag="VE030_01535"
     CDS             complement(<1..1720)
                     /locus_tag="VE030_01535"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZF22129.1"
                     /translation="MIVLRDLQLRRGAKVLFEGASLTFNRGQKIGVTGANGSGKSSLF
                     ALLLGEIHQDAGEFEIQPGLVLAHVAQETPATDQAAIDYVLDGDAELRQLEGDLLEAE
                     RAHNGTRIAELHEDMQRIGAYSARARAAQLMHGLGFAYSEIDRPVAQFSGGWRVRLNL
                     ARALMCRSDILLLDEPTNHLDLDAIIWLEQWLSSYPGTLLLISHDRDFLDAVAGAICH
                     IEGQRLRLYAGNYSAFETQRAAQLAQQQATFLKQQREIAHLQSFVDRFKAKATKARQA
                     QSRIKALARMEKIAAAHVDTPFEFAFRQPAVQPDPVLTLDGVEAGYGDVTVLSGIRMT
                     LRAGTRLGLLGRNGAGKSTMMKLLAGVLAPLTGGRVEGKGLQIGYFAQHQLEQLRPEE
                     SPLWHLTRLSPRTREQELRNFLGGFDFHGDQVTEPVAPMSGGEKSRLALALIVWQRPN
                     LLLLDEPTNHLDLEMRHALTLGLQDYQGALVLVSHDRSLLRATADELRLVEGGTVEPF
                     DGDLEDYARRLRAKEQADSAAAEPVVGRKEQKRLEAEERSRRFARRKPLQARLRSAER
                     EIEALGI"
     gene            complement(1760..1835)
                     /locus_tag="VE030_01540"
     tRNA            complement(1760..1835)
                     /locus_tag="VE030_01540"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1800..1802),aa:His,seq:gtg)
     gene            complement(1946..2022)
                     /locus_tag="VE030_01545"
     tRNA            complement(1946..2022)
                     /locus_tag="VE030_01545"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1986..1988),aa:Arg,seq:tct)
     gene            complement(2053..2129)
                     /locus_tag="VE030_01550"
     tRNA            complement(2053..2129)
                     /locus_tag="VE030_01550"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2093..2095),aa:Pro,seq:tgg)
     gene            2275..2697
                     /gene="dksA"
                     /locus_tag="VE030_01555"
     CDS             2275..2697
                     /gene="dksA"
                     /locus_tag="VE030_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HZF22130.1"
                     /translation="MATEFHTNFKPYVPKKGEAYMSPKQLDHFRKMLNQLKIELSQDI
                     DRTVHTMQDEATVFADPNDRASQESDMALELRNRDRERKLIKKIDETIAKIDSGEYGY
                     CESCGIEIGLKRLEARPTATLCIDCKTLDELREKQVAK"
     gene            2952..3365
                     /locus_tag="VE030_01560"
     CDS             2952..3365
                     /locus_tag="VE030_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZF22131.1"
                     /translation="MNRHLRNFVVAARDKYMSAIPGPQPVTRQLYGHLEMKMFAKIQN
                     PRGIRRRLGMNQQEFWGRIGVTQSGGSRYESGRNMPKPVQELLRVVHVGQIDLNKLNK
                     GDVAVISYLKTAEPALYRRLKTEASGHMTRRRGRR"
     gene            complement(3369..4232)
                     /locus_tag="VE030_01565"
     CDS             complement(3369..4232)
                     /locus_tag="VE030_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22132.1"
                     /translation="MSESRRGRLTVADHDRGSAGLTYVYPVVSRRAGGVSVGINLNVN
                     NACNWRCIYCQVPGLTRGAPPPVDLVKLERELSGFLQDVLHGDFLAARVPVEARRLND
                     IAISGNGEPTSAGEFESVIDLVGEVRRQTAVPAEVKTVLITNGSLMQRAGVQAGVRKL
                     AALSGEVWFKVDRATRSGLRKVNNTRADIARMRANLEIAARLCPTWIQTCVFAIDGVP
                     PEGAEQQAYLGFLRERVAEGLPLQGVLLYGLARQSFQPEAKHLGALPFAWLDAFAEKI
                     RALGLKVKVTP"
     gene            4657..5289
                     /gene="tpx"
                     /locus_tag="VE030_01570"
     CDS             4657..5289
                     /gene="tpx"
                     /locus_tag="VE030_01570"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF001808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HZF22133.1"
                     /translation="MKNVFVASLALASLLGLAAGVASAAGEEVQGIKVNKENGSPGDG
                     NNVLMKGSPLPLRGHAVKVGEPLPAAIVTGANLGPVNMADGSGKVRIISVVPSVDTPT
                     CEAQTHLLSEKDRKLADQVEMVTVSMDLPFAQQRFAKEAKIKNITFYSDYKSGEFGMN
                     NGLMIEPLHLLARAVIVTDKNNVVRYIQVVPEVTQLPDMQAAMNFAKTLL"
     gene            complement(5999..6829)
                     /locus_tag="VE030_01575"
     CDS             complement(5999..6829)
                     /locus_tag="VE030_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZF22134.1"
                     /translation="MSNQTPAGAARLDGRVAIVTGGTQGLGEAIASLFVERGAKGLVI
                     CGRDADKGRAVAKRLGARGCRTEFVQADLAALADCRKVVAAADTAFGRVDVLINVAGL
                     TDRGTIWDTSPELFERMFAVNVRAPFFLMQDVLKIMRREKIKGTVVNILSMSAHGGQS
                     FITAYCGSKGALATLTKNVGFSVMRHGIRVNGLCIGWMDTPGEDRIMRLYHGARDGWK
                     EEAEAKLPTGRLLKVDEVAKAAAFLASDESGMMTGSIVDFDQSVLGCYESAPQPAPND
                     "
     gene            complement(6852..>7750)
                     /gene="iolE"
                     /locus_tag="VE030_01580"
     CDS             complement(6852..>7750)
                     /gene="iolE"
                     /locus_tag="VE030_01580"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010397347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HZF22135.1"
                     /translation="KVRFGIAPIAWTNSDLPELGGNTALETCLAESREAGFSGTETGV
                     KFPMDATSLRSVLQRFTLELVSGWFSGELLQRSVDQEKDRIEAQLATFKALGASVLVY
                     AETTGSVQSRIDAPLSSRPRLAAEQFRGYGRKLTALAEHMAERGVRMTYHHHMGTVVE
                     TQREIDLLMENTGSAVGLLIDTGHLTYAGADVVETTRRHARRINHVHCKDIRAGILKT
                     ARRNDQSFLRAILEGVFTVPGDGFIDYYAFARVLADIGYEGWAVVEAEQDPVKAPPLE
                     YSRIGLHHLMAAFEAAGFDFVK"
BASE COUNT         1552 a         2468 c         2284 g         1446 t
ORIGIN      
        1 cgatcccgag cgcctcgatt tcccgctccg ctgatcttag ccgtgcctgc agcggtttgc
       61 gcctggcaaa gcgccggctg cgttcctcgg cttcgagccg tttctgctcc ttgcggccga
      121 cgacaggttc tgcggcagcg ctatcggcct gttccttcgc gcgcaaccgt ctggcgtaat
      181 cctccaggtc gccgtcgaag ggttcgaccg tgccgccctc caccagccgc agttcgtcgg
      241 cggtcgcgcg cagcaggctg cggtcgtgcg acaccagcac cagcgcgcct tggtaatcct
      301 gcagcccgag ggtcagcgca tggcgcattt ccaggtccaa atggttggtg ggctcgtcca
      361 gcagcagcag gttcggccgt tgccagacga tgagcgccag ggcgagccgg gacttttccc
      421 ctcccgacat cggcgccacc ggctcggtca cctgatcgcc gtggaaatcg aagccaccga
      481 gaaaattgcg cagctcctgc tcgcgcgtcc tgggcgacag ccgcgtcagg tgccacagcg
      541 ggctctcctc gggacgcaac tgctcgagct ggtgctgcgc gaagtaaccg atctgcagtc
      601 ccttgccctc gacccgccct ccggtgagcg gcgccagcac gcccgccagt aacttcatca
      661 tcgtcgactt gccggcgccg ttgcggccga ggagccccag ccgggtgccg gcgcgcagcg
      721 tcatccggat tccgctcagg acggttacat cgccgtagcc ggcttcgacc ccgtccagcg
      781 tcagcaccgg gtcgggctgc actgcgggct gccggaacgc gaactcgaac ggcgtatcca
      841 cgtgcgccgc cgcgattttc tccatgcggg cgagcgcctt gatgcggctc tgcgcctggc
      901 gcgccttggt ggccttggcc ttgaatcggt cgacgaagga ctgcaggtgg gcgatttcgc
      961 gctgctgctt gaggaaagtg gcctgctgct gcgccagttg cgcggcgcgc tgggtctcga
     1021 acgcggaata gtttcccgca tacagtcgga ggcgctggcc ttcgatgtgg caaatggcgc
     1081 cggccaccgc atcgagaaaa tcgcggtcgt gcgagatcag cagcagcgtc cccgggtagc
     1141 tggaaagcca ctgctcgagc cagataatgg catcgaggtc gaggtgattg gtcggttcgt
     1201 ccagcagcag aatgtccgag cggcacatca gcgcgcgcgc caggttcagc cgcacgcgcc
     1261 agccgccgga gaactgcgcg accggcctgt cgatttcgga atacgcgaag ccgaggccat
     1321 gcatcaactg cgctgcgcgg gcacgcgccg aataagcgcc gatgcgctgc atgtcttcgt
     1381 gcagttcggc gatgcgcgtg ccgttgtgcg cccgctccgc ctccagcaga tcgccttcga
     1441 gttgccgcag ttccgcgtcg ccgtcgagca cataatcgat cgcggcctga tcggtggcag
     1501 gagtttcctg cgcaacgtgc gcgagcacca gcccgggctg aatttcgaat tcacccgcgt
     1561 cctggtgaat ttcgccaagc agaagcgcga acaggctgga cttaccgctg ccgttggcgc
     1621 cggtgacgcc gatcttctga ccgcgattga aggtgaggct cgcgccctcg aacagcactt
     1681 tggcgccgcg gcgaagttgc aggtcgcgca gaacgatcat gacttgcgga tcaaaggtcg
     1741 aagcggaagg aaacgcagat ggggtggctg atgggactcg aacccacgac aaccggaatc
     1801 acaatccggg actctaccag ctgagctaca gccaccgtcc gagaaggcaa tgtcaagttt
     1861 tgagttttga gtgttaagtg accgtgagca ccagagaacg tcccactcaa cactcaacat
     1921 tcaacactgc ccggatgggc aaacctggtg tgcccgacag gaatcgaacc tgtaaccccc
     1981 agcttagaag gctggtgctc tatccggttg agctacgggc acgactctta accgggcacg
     2041 actctcgcta tctggtcggg gtggagagat tcgaactccc gacatcctgc tcccaaagca
     2101 ggcacgctac caggctgcgt tacaccccgg agggtcgcaa ctataccgat cagggggtta
     2161 aaggtcaatt tgaaggtcct gaaggcattg cgttttcgcc gcgcagaaac taagcttgca
     2221 cccccctgct atttctgcta ttaaagcgat tttttttcgg accgaaactg gcgaatggcg
     2281 accgaatttc acacgaattt caagccttat gtgccgaaga agggcgaggc ttacatgagt
     2341 ccaaagcagc tcgatcactt caggaaaatg ctcaaccaac tgaagatcga gctctcgcag
     2401 gatatagacc gcaccgtgca cacgatgcag gacgaagcga cggtgttcgc cgatccgaat
     2461 gatcgtgcca gccaggaatc ggatatggcg ctggaactgc gcaatcgcga tcgcgagcgc
     2521 aagctgatca agaaaatcga tgaaacgatc gccaagatcg actccggcga atatggctac
     2581 tgcgaaagct gtggcatcga gatcggcctg aagcgcctgg aagcaagacc cactgccacc
     2641 ctctgcatcg actgcaagac cctcgacgag ctcagagaaa agcaagtggc gaagtaactt
     2701 caccgccctt gctttcagtg aaggctaacc tgcccctggc aggttatgcc ggaactacaa
     2761 tccccacgcg aagtaacttc gccgcccttg ctctcagtga aggctaacct tcccctggca
     2821 ggttatgcca gaactaaaag caggtggcca agcaacaccc ggccgctcaa cctcccgcta
     2881 tccgatacac gtcaacgccc cgcccatgcg gggcgttgac gttttccatc aggcgccgct
     2941 aggcgcttgt catgaaccgc catctgcgga actttgtggt tgcggcgcgg gacaaataca
     3001 tgtcggcaat ccccggtccg caaccggtta ccagacaact ttacggccat ttggagatga
     3061 aaatgtttgc gaaaattcag aaccctcggg gtatccgccg gcgcctcggc atgaatcaac
     3121 aggaattctg gggacgcatc ggcgtgaccc aaagcggcgg ttcgcgctac gaaagcggcc
     3181 gcaacatgcc gaaaccggtg caggaactgc tgcgcgtagt gcacgtgggg cagatcgacc
     3241 tgaataaact caacaaaggc gacgtcgccg tgatttcgta tctgaagacc gccgagccgg
     3301 cgctgtatcg ccggctcaaa accgaagcct ccggtcacat gacgcggcgg cgcggccgcc
     3361 gctaaccctc agggtgtaac ctttaccttg aggcccagcg cgcggatttt ttccgcaaag
     3421 gcgtccagcc acgcaaacgg gagtgcgccc aggtgcttgg cttccggctg aaacgattgc
     3481 cgcgccagtc cgtacagcag cacgccctgc agcggcaatc cttcggctac acgttcgcgc
     3541 aggaaaccga ggtaggcctg ttgctcggcg ccttcgggtg ggaccccgtc gatcgcgaac
     3601 acgcaggtct gtatccaggt cgggcacaga cgcgccgcaa tttccaggtt tgcgcgcatc
     3661 ctggcgatat ccgcacgggt attgttgact ttgcgcagac ccgatctggt ggcacgatcc
     3721 accttgaacc aaacctcacc gctgagcgct gccagcttcc tgactcccgc ctgaacgccg
     3781 gcgcgctgca tcagactgcc attggtgatc agcaccgtct tgacctccgc cggcacggcg
     3841 gtttgtcgcc gtacctcccc gaccaggtct atcacgctct cgaattcccc ggcactggtc
     3901 ggctccccat ttccggaaat cgcaatatcg ttcaggcgac gcgcctcgac cggcacgcgc
     3961 gcagcaagga aatcgccgtg caacacgtct tgcagaaatc cggatagttc ccgctccagc
     4021 ttgacgagat ccaccggcgg cggcgcgcca cgggtgagac ccggaacctg acagtagatg
     4081 caacgccaat tgcaggcatt gttgacgttg agattgatgc ccacggaaac accacccgcg
     4141 cgccgcgaca ccaccggata cacgtaggtc agtcccgcgc tcccgcggtc gtgatcggca
     4201 accgtcaagc ggccgcgtcg tgattcggac atgtgtcgtc gcagtttgac gttgaagcgc
     4261 ctccggagaa gctcaagtgt attccggttt ccaatttaga attcgtagct tacgagaaag
     4321 agcgggcccc cggaacggcg tatggccacc cctgctcggc tttcggactg ccccagtgtt
     4381 cattcgcaat agcccgtcca tattgcatta tcgagaccgt acgcggtacc ggcaagaaat
     4441 ggccacgccc agccccaggg aaaagcttcg cgagcatgtc gctggcggct ggggacggcg
     4501 atccggccgc acgcagatgc tcgcgattgt cccggatcat gattatcatg agcgtgaaat
     4561 cagcgtttcc gtctgggacc atgcgctcct gagcaccaga atcgcaaggc tgcacggagc
     4621 taaagaggag caataacaat tttggaggac actgcaatga agaatgtatt tgtcgcatcg
     4681 ctcgcgctgg cgagcctgtt gggactggcc gcgggcgttg cgtccgctgc cggcgaggaa
     4741 gtccagggca tcaaggtcaa caaggaaaac ggcagtcccg gcgacggcaa caacgtactc
     4801 atgaaaggca gcccgctgcc gttgcgcggt catgcggtca aggtgggtga gccgcttccc
     4861 gccgcgatcg ttaccggcgc caaccttggg ccagtcaata tggccgatgg aagcggcaag
     4921 gtgcgcatca tcagcgtcgt accctcggtc gacacgccga cttgcgaggc acagacccac
     4981 ctgctcagcg agaaggaccg caagctcgcc gaccaggtcg aaatggtgac ggtgagcatg
     5041 gacttgccgt tcgcgcagca gcgttttgcc aaagaggcaa agatcaagaa catcacgttc
     5101 tactcggatt acaagtccgg ggagttcggg atgaacaacg gcttgatgat cgagccgctc
     5161 cacctgctgg cgcgcgccgt gatcgtcacc gacaagaata acgtggtgcg ctacatccag
     5221 gtcgtgccgg aagtcaccca gcttcccgac atgcaggccg cgatgaactt cgcgaagaca
     5281 ctgctgtaaa cgatccggta ctgcaacaca aaacggcccc gcgctcggcg gggccgtttg
     5341 tttgtggcgg tggtcgcagt ctcaagcgaa cccgtctcca gcccaaattc cctgctaaca
     5401 gggaaaaata cagggaattt cgcacgtttt tgcagttcac atcgcgcccc actccgccga
     5461 gactcgcatt cagagcgact ctcagggcat ctaccccgtc tccgggcacg gtgcggaaca
     5521 gggaattaat tcgggcgtat cagggaattt atcgggaaga acagggaatt ttttctccac
     5581 gccgtctacg gataaattcg ccggcactat ttaagacacc aatggccgct tgactaccaa
     5641 ttcgccttcc atacgcagct gcgaactccg atgacattgc aatccggcac gtcttagcca
     5701 aaacgacaaa taaagggcgc gactcgcgtg acgccctttg cgtttgcttc agcactcagg
     5761 tccaggtaac cctctatttt cgggtttaac gtggtcgcta cccgttcgca tcaggcacgc
     5821 cgaacgaaat ggcacgcaca acagcgaagg tcttgggcaa caaccagatc gtcatgcctt
     5881 gggcttgaga atctaccccg gcccagctcg cagaagcgaa tccagtaatc ccccttcgtc
     5941 cgtcaaagcc aacggatttc tgctcctaat ttggcggatc ttgtggtccc accaggtgtc
     6001 agtcgttcgg tgcgggctgc ggcgccgatt cgtagcaccc cagtaccgac tggtcgaaat
     6061 cgacaatcga ccccgtcatc attcccgact cgtcggaggc gagaaaagcg gctgccttgg
     6121 caacctcgtc gaccttcaac aaacggccgg ttggaagctt cgcctccgcc tcttccttcc
     6181 atccatcccg ggcgccgtgg tagagccgca tgatgcggtc ttctcccggc gtgtccatcc
     6241 agccgatgca caggccgttg acgcggatgc cgtggcgcat gacgctgaac ccaacgttct
     6301 tggtcagcgt ggcaagcgcg cccttcgagc cgcagtaggc agtaatgaaa gactgcccac
     6361 cgtgggccga catgctcagg atgttgacga cggttccttt tattttttcg cgtcgcatga
     6421 ttttgagcac gtcctgcatc aggaaaaacg gtgcccgtac gttgaccgcg aacatccgtt
     6481 cgaagagttc gggcgaggtg tcccagatcg tgccgcgatc ggtcaagccg gcaacgttga
     6541 tcaagacatc gacgcgaccg aatgccgtat cggcagccgc gaccactttg cggcagtcgg
     6601 caagcgcggc aaggtccgcc tgtacgaatt cggtcctgca gccgcgcgcg ccgaggcgct
     6661 tggccaccgc gcgccccttg tctgcgtcgc ggccgcagat caccagcccc ttggcgccgc
     6721 gttcgacgaa caggctggcg atcgcctcgc ccagcccctg ggttccgccg gtcacgatcg
     6781 cgacgcgacc atcgagccgc gccgcgccgg cgggtgtctg attgctcatg attcgttcct
     6841 cgtggagtta ctcatttgac gaaatcgaat ccagccgcct cgaacgcggc catgagatgg
     6901 tgcagcccga tgcgtgaata ctccagcggc ggcgccttga ctggatcctg ctcggcctcc
     6961 accacagccc acccctcgta accaatgtcg gcaagcactc gcgcgaaggc gtaatagtcg
     7021 atgaagccgt cgccgggcac ggtgaaaacg ccttccagaa tcgcccggag gaaactctgg
     7081 tcgttcctgc gggcagtctt caatatccct gcgcggatgt ctttgcaatg cacatggttg
     7141 atccgccgcg catgccggcg cgtagtctcc accacatcgg cccccgcgta ggtcagatgc
     7201 ccggtgtcga tcagcagacc gacggcggac ccggtgtttt ccatcaacag gtcgatctcc
     7261 cgctgggtct cgacgacggt gcccatgtgg tggtggtagg tcatgcgcac gcctcgctcc
     7321 gccatgtgct ccgccagtgc agtcagtttg cggccgtagc cccggaactg ctcggcagcc
     7381 aggcgaggcc gactcgacag cggcgcgtcg atgcgtgact ggacgctgcc ggtggtctcc
     7441 gcgtaaacca gcactgaggc cccgagcgcc ttgaaggtcg cgagctgcgc ctcgatgcga
     7501 tccttttcct ggtccaccga gcgctggagc aattcaccgg aaaaccagcc ggagaccagc
     7561 tcgagcgtga agcgttgcag gaccgaccgc agcgaggtcg cgtccatcgg aaacttgacc
     7621 ccagtctcgg tgccgctgaa accggcttca cggctttccg ccaggcaggt ttccagcgcc
     7681 gtgttgccgc caagctcggg caggtcgctg ttggtccagg caatcggtgc gatgccgaaa
     7741 cgaaccttca
//
LOCUS       DASQOP010000041         5507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1626593, whole genome shotgun sequence.
ACCESSION   DASQOP010000041 DASQOP010000000
VERSION     DASQOP010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5507
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1626593"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..171
                     /gene="secY"
                     /locus_tag="VE030_01585"
     CDS             <1..171
                     /gene="secY"
                     /locus_tag="VE030_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070586.1"
                     /note="forms heterotrimeric complex in the membrane; in
                     bacteria the complex consists of SecY which forms the
                     channel pore and SecE and SecG; the SecG subunit is not
                     essential; in bacteria translocation is driven via the
                     SecA ATPase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HZF22136.1"
                     /translation="LIVKWNVPFYFGGTSLLIIVVVTMDFMAQVQAYVMSHQYESLLK
                     KANFKGGMFPTR"
     gene            181..399
                     /gene="infA"
                     /locus_tag="VE030_01590"
     CDS             181..399
                     /gene="infA"
                     /locus_tag="VE030_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HZF22137.1"
                     /translation="MAKEETIQMQGEILETLPNATFRVKLENGHVVLGHISGKMRMHY
                     IRILPGDKVTVELTPYDLTRARIVFRAK"
     gene            457..570
                     /gene="rpmJ"
                     /locus_tag="VE030_01595"
     CDS             457..570
                     /gene="rpmJ"
                     /locus_tag="VE030_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HZF22138.1"
                     /translation="MRVQASVKKMCRNCKIVRRKRVVRVICTDPRHKQRQG"
     gene            608..1003
                     /gene="rpsM"
                     /locus_tag="VE030_01600"
     CDS             608..1003
                     /gene="rpsM"
                     /locus_tag="VE030_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HZF22139.1"
                     /translation="MARIAGVNIPNHQHAEIALTAIYGIGRARARAICAAAGVTFSTK
                     MKDLSDNDMDKLREQVAKFTVEGDLRRELSMNIKRLMDLGCYRGVRHRRGLPVRGQRT
                     RTNARTRKGPRKAIQRSASSASAPAAAPK"
     gene            1045..1446
                     /gene="rpsK"
                     /locus_tag="VE030_01605"
     CDS             1045..1446
                     /gene="rpsK"
                     /locus_tag="VE030_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HZF22140.1"
                     /translation="MAKVTTTPAARVRKKVKKNVAEGIAHIHASFNNTIITITDRQGN
                     ALSWATSGAAGFKGSRKSTPFAAQVAAEQAGRAAQECGVKNLEVRIKGPGPGRESAVR
                     ALNAVGFKITSISDVTPVPHNGCRPPKKRRI"
     gene            1463..2089
                     /gene="rpsD"
                     /locus_tag="VE030_01610"
     CDS             1463..2089
                     /gene="rpsD"
                     /locus_tag="VE030_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HZF22141.1"
                     /translation="MARNLDPKCRQCRREGEKLFLKGEKCFTDKCAIERRSYAPGQHG
                     QKNLRRSDYGTQLREKQKIRRIYGLLERQFRNTYKEAARAKEVTGEALLQMLESRLDT
                     VAYRMGFGASRTEARQIVRHNGILVNGKRVNIPSYQVRQGDVIEVAQKAKSQLRIKAA
                     TEAAEGRGYPEWIEVDIKALKGTFKAKPQRSELPATINESLVVELYSK"
     gene            2188..3162
                     /gene="rpoA"
                     /locus_tag="VE030_01615"
     CDS             2188..3162
                     /gene="rpoA"
                     /locus_tag="VE030_01615"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HZF22142.1"
                     /translation="MQSSFLKPRIIDVQTISPFHAKVTMEPFERGYGHTLGNALRRVL
                     LSSMPGFAPTEVKINGVLHEYSTIDGVQEDVVDILLNLKGIVLKLHNRDEVVLNLKKQ
                     GESVVTAGDIEGSHDVEIINPDHVIAHIAPGGKLDMQIKVEGGRGYVPATTRAAAKEG
                     RVIGNIMVDASFSPVRRVSYAVESARVEQRTDLDKLIMDIETNGVVEPEEAIRYAARI
                     LVDQLSLFADLKGTPAPIEQPKAPQVDPILLRPVDDLELTVRSANCLKAENIYYIGDL
                     IQRTETELLKTPNLGRKSLNEIKEVLASRGLTLGMKLENWPPVGLEKV"
     gene            3265..3657
                     /gene="rplQ"
                     /locus_tag="VE030_01620"
     CDS             3265..3657
                     /gene="rplQ"
                     /locus_tag="VE030_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HZF22143.1"
                     /translation="MRHRLGLRKLNRTSSHRLAMLRNMTNSLLRHEMIKTTLPKAKEL
                     RRVAEPIITLGKKPSLANRRLAFSRLRDRDMVMKLFNELGPRYAKRNGGYLRILKFGF
                     RQGDNAPMALVELLDRPAETEEAKPTEK"
     gene            complement(3726..>5507)
                     /gene="uvrA"
                     /locus_tag="VE030_01625"
     CDS             complement(3726..>5507)
                     /gene="uvrA"
                     /locus_tag="VE030_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZF22144.1"
                     /translation="YVGERGNKYQREHPFEGIVPNLERRYRETDSIVVREELAKFLAS
                     QPCPECNGTRLRREARYVFVAGKPIYEVSAMPLKRALQFFQETGFEGARLAIADKIVR
                     EIANRLQFLVNVGLDYLALDRSADTLSGGEAQRIRLASQIGSGLTGVMYVLDEPSIGL
                     HQRDNDRLLATLKELRDLGNSVIVVEHDFEAIMSADYVVDMGPGAGEHGGHIVAEGTP
                     QAMMAHTGSLTAQYLSGRRQIAIPRLRHKPQAKNTLRLIGARGNNLKGVTLDLPVGLL
                     VCVTGVSGSGKSTLINDTIYHSVAQHLYGSAAEPAPFDEIQGIDFFDKVVNVDQSPIG
                     RTPRSNPATYTGLFTPIRDLFAGVPLARERGYGAGRFSFNVKGGRCEACQGDGVIKVE
                     MHFLPDIYVPCDVCHGRRYNRETLEIQYKSRNIHEVLEMTVEQAHAFFSAVPVIARKL
                     DTLLDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRDTGRTLYILDEPTTGLHFQDI
                     EMLLKVLHRLRDHGNTVVVIEHNLDVIKTADWVVDLGPEGGDGGGRIVAVGTPEEIAR
                     HPDSYTGRYLRPVLGLDKVEKRASGAR"
BASE COUNT         1109 a         1695 c         1748 g          955 t
ORIGIN      
        1 ctgatcgtga agtggaacgt gccgttctac ttcggcggca ccagcctgct gatcatcgtg
       61 gtggtgacga tggatttcat ggcccaggtg caggcctacg tgatgtcgca ccagtacgag
      121 agcctgctca agaaggcgaa cttcaagggc gggatgttcc cgacccgtta acggaggagc
      181 atggccaagg aagagacgat ccagatgcag ggcgaaattc ttgagaccct gccgaacgcg
      241 acgtttcgcg tgaagctgga gaacggccat gtggtactgg gacatatctc cggcaagatg
      301 cgcatgcact acatccgcat cctgcccggg gacaaggtga cggtggagtt gacgccttac
      361 gatctgacgc gcgcgcgcat cgtattccgc gccaagtaag gccgcccgct ccggttcgag
      421 cgcagacagg catgcagagc agttgaagga gagaaaatga gagtacaggc atcggtcaag
      481 aagatgtgcc ggaactgcaa gatcgtccgg cgcaagcgcg tggtgcgcgt gatctgcacg
      541 gacccgcgcc acaagcaacg acagggttaa acgggcggca acgccacttt acttaggacg
      601 cacgactatg gctcgtatcg ccggtgtaaa cattccgaac catcagcatg ccgagatcgc
      661 gttgaccgcg atttacggca tcgggcgcgc gcgcgcgcgc gcgatctgcg ccgccgccgg
      721 ggtcactttc tcgaccaaga tgaaggatct gtccgacaac gacatggaca aactgcgcga
      781 acaagtcgcc aaattcactg tcgaaggcga cctgcggcgc gaactgtcaa tgaacatcaa
      841 gcggctgatg gacctcggct gctaccgcgg ggtgcgccac cgccgtggcc tgccggtgcg
      901 cggtcagcgt acccgtacca atgcccggac ccgcaagggc ccgcgcaagg cgatccagcg
      961 cagcgccagc tcggcttcag ctccggccgc cgctccgaag taaacgtggc gaagtaaccg
     1021 catcgagtac agggatttca agacatggca aaggtaacga ctaccccggc agcccgggtc
     1081 aggaagaagg tcaagaagaa tgtggccgaa ggcatcgccc acattcacgc ctccttcaac
     1141 aacaccatca tcaccattac cgaccgccag ggtaatgccc tgtcgtgggc gacttccggg
     1201 gccgccggct tcaagggctc gcgcaagtcg actccgttcg ccgcccaggt cgctgccgaa
     1261 caggccggcc gcgcggcgca ggaatgcggc gtgaagaatc tggaagtgcg catcaagggc
     1321 cccggaccgg ggcgcgaatc cgcggtgcgg gcgttgaacg cggttggttt caagatcacc
     1381 agcatctcgg acgtgacgcc ggtaccgcac aacggctgcc gcccgcccaa gaagcgccgt
     1441 atctgatcga aggagacttg tagtggcaag aaatctggat cccaagtgcc gccagtgccg
     1501 ccgtgagggg gagaaactct tcctcaaggg cgagaaatgc ttcaccgaca agtgtgccat
     1561 cgagcgccgt tcgtacgcgc ccggccagca cggccagaag aacctgcgcc gttccgacta
     1621 tggcacgcag ttgcgcgaga agcagaagat ccgccgcatt tacgggctgc tggagcgcca
     1681 gttccgcaat acttacaagg aagccgcgcg cgccaaggaa gtgaccggcg aggcgttgct
     1741 gcaaatgctc gaaagccggc tcgacaccgt ggcctaccgg atgggcttcg gcgcctcgcg
     1801 caccgaggcg cgccagatcg tgcgccataa cggcatcctg gtgaacggca agcgggtcaa
     1861 cattccgtcc taccaggtgc gccagggcga cgtgatcgaa gtggcgcaga aggcgaagag
     1921 ccagctgcgc atcaaggcgg cgaccgaagc cgccgaaggc cgcggttacc ccgaatggat
     1981 tgaagtggac atcaaggcgc tcaaaggcac gttcaaggcg aagccgcaac gttccgaact
     2041 gcccgccacc atcaacgaat cgctggttgt ggaactgtac tcgaagtaac cgcggcctga
     2101 tcgcggcccg ccgatcccga agttgaccgg ccgggccgtg cgtttttggg ccgtaacgat
     2161 ccgaatttct cgtagagggt cacctttatg caaagcagct ttctgaagcc gcgaattatc
     2221 gatgttcaga ccatttcgcc tttccacgcg aaagtgacga tggagccgtt cgagcgcggc
     2281 tatggccata cgctgggcaa cgcgctgcgc cgggtgctgc tgtcctcgat gcccggcttc
     2341 gcgccgactg aagtgaagat caacggcgtg ctgcatgagt actccacgat cgacggcgtg
     2401 caggaagacg tcgtcgacat cctgctcaac ctcaagggca tcgtgctgaa actgcacaac
     2461 cgcgacgagg tggtgctgaa cctgaagaag cagggcgaga gcgtggtgac cgcgggagat
     2521 atcgaaggct cgcacgacgt cgagatcatc aatcccgatc acgtgatcgc gcatatcgct
     2581 ccgggcggca agctcgacat gcagatcaag gtcgagggtg gccgcggtta cgtgccggcc
     2641 accacccggg cggctgccaa ggaaggtcgc gtcataggca atatcatggt ggacgcctcg
     2701 ttcagtccgg tgaggcgggt gagctacgcg gtcgagtccg cgcgcgtcga gcagcgcacc
     2761 gacctcgaca agctgatcat ggacatcgag accaacggcg tggtcgaacc cgaggaagcg
     2821 atccgctacg cggcgcgcat cctggtcgac cagctgtcgc tgttcgcgga cctcaagggc
     2881 acgccggcgc cgatcgagca accgaaggcc ccgcaggtgg acccgatcct gctgcgcccg
     2941 gtggacgacc tggaactcac ggtgcgctcg gccaactgcc tcaaggcgga gaacatctat
     3001 tacatcggcg acctgatcca gcgcaccgag accgagctgc tgaagacgcc gaacctcggc
     3061 cgcaagtcgc tcaacgagat caaggaagtg ctcgcctccc gtggattgac cctggggatg
     3121 aaattggaga actggcctcc ggtcggcctg gagaaagttt aagaacaagt atgaaggccg
     3181 aaggcaaaag gtggacgtgc ccccgtcgcg ggtgtttctt catccttcat ccttcatctt
     3241 tcatcctttg atgaggaacg aatcatgcgc caccgtctgg gactgagaaa actcaaccgc
     3301 accagcagcc atcgcctggc gatgctgcgc aacatgacca actcgctgtt gcgccacgag
     3361 atgatcaaga ccacgttgcc gaaggcgaag gagttgcgcc gcgtggccga gcctatcatc
     3421 acgctgggca agaagccctc gctcgctaac cggcgcctgg cattttccag gctgcgcgat
     3481 cgcgacatgg tgatgaagct attcaacgaa ctgggtccgc gctacgccaa gcgcaacggc
     3541 ggctatctgc gcatcctcaa gttcggtttc cgccagggtg ataacgcacc gatggcattg
     3601 gttgagttgc tcgatcggcc tgcggagact gaagaagcca agcccactga aaagtaaggc
     3661 agcggcagat catcgaaaga aaaccgggca gtgcccggtt tttgttttct gcgccggcgc
     3721 gggaatcagc gcgcgccgga tgcgcgcttc tcgactttgt ccaggccgag caccggccgc
     3781 aggtagcggc ccgtgtagct gtcgggatgg cgggcaattt cctccggagt gcctacggcg
     3841 acaatgcgcc cgccgccatc gccgccctcc ggaccgaggt ccacgaccca gtcggcggtc
     3901 ttgatcacat cgaggttgtg ctcgatcacc accaccgtat tgccgtggtc gcgcaagcgg
     3961 tgcaatacct tcagcagcat ctcgatgtcc tggaaatgca gcccggtggt gggctcgtcg
     4021 agtatgtaga gcgtgcgccc ggtgtcgcgt ttcgacaatt ccagcgccag tttcacccgc
     4081 tgcgcctcgc cgccggaaag cgtggtggcg ctctggccga gacggatgta accaagtccg
     4141 acgtcgagca gcgtgtcgag cttgcgcgcg atcaccggta cggcgctgaa gaacgcgtgc
     4201 gcctgctcca ccgtcatttc cagcacttcg tggatgttcc tgctcttgta ctggatctcc
     4261 agcgtctcgc ggttgtagcg gcggccatgg cagacatcgc agggcacgta gatgtcgggg
     4321 aggaaatgca tctccacttt gatgaccccg tcgccctggc acgcctcgca gcggccgcct
     4381 ttgacgttga aggagaagcg ccccgctccg taaccgcgct cgcgcgccag cggcactccg
     4441 gcgaacaggt cgcggatcgg cgtgaacagc ccagtgtaag tggcgggatt ggaacgaggg
     4501 gtgcggccga tcgggctctg gtcgacgttc accaccttgt cgaagaaatc gattccctgg
     4561 atctcgtcga acggtgccgg ttcggcggcg ctgccataga gatgctgcgc caccgagtga
     4621 tagatcgtat cgttgatcag ggtggacttg cccgatcccg agacgccggt cacgcacacc
     4681 agcagcccga ccggaagatc gagggtcaca cccttgagat tgttgccgcg cgcgcctatc
     4741 agccgcagcg tgttcttggc ctgcggcttg tgccgcaggc gcgggatggc gatctgcctt
     4801 ctgccggaca aatactgggc ggtgagtgac ccagtgtgcg ccatcatggc ctgaggcgtg
     4861 ccttcggcga cgatatggcc gccgtgctcg ccggccccgg gtcccatatc caccacgtaa
     4921 tccgcgctca tgatcgcctc gaagtcgtgc tcgaccacga tgaccgagtt gcccagatcg
     4981 cggagttcct tgagggtggc gagcagccgg tcgttgtcgc gttggtgcag accgatcgac
     5041 ggttcgtcca gaacgtacat cacgccggtg agaccggagc cgatttgcga ggccagcctg
     5101 atgcgctgtg cttcgccgcc cgaaagcgtg tctgcggagc ggtcgagtgc aaggtaatcg
     5161 aggccgacgt tgaccaggaa ctgcagccga ttggcgattt cgcgcacgat cttatcggcg
     5221 atcgccagcc tggcgccctc gaacccggtc tcctggaaga actgcagcgc ccgtttcagc
     5281 ggcatggcgc tcacttcgta gatcggcttg ccggcaacga agacgtagcg cgcttcgcgg
     5341 cgcagccgcg tgccgttgca ctcggggcag ggctggctgg cgaggaactt tgccagttcc
     5401 tcgcgcacca cgatcgaatc cgtttcgcga tagcgccgtt ccagattcgg cacgatgcct
     5461 tcgaacgggt gttcgcgctg gtacttgttg ccgcgctcgc cgacgta
//
LOCUS       DASQOP010000042         9176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2433395, whole genome shotgun sequence.
ACCESSION   DASQOP010000042 DASQOP010000000
VERSION     DASQOP010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9176
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2433395"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..1105
                     /gene="dusB"
                     /locus_tag="VE030_01630"
     CDS             98..1105
                     /gene="dusB"
                     /locus_tag="VE030_01630"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HZF22145.1"
                     /translation="MHIGPHVLKNNLIVAPMAGVTDRPFRQLCKAMGAGMAVSEMIAA
                     NPQLWNTAKSQRRSDHRGEVDPISVQIAGADPQMMADAARYNVQRGAQIVDINMGCPA
                     KKVCNVAAGSALLRDEQLVGKILDSVVAAVEVPVTLKIRTGWDAHNRNAVRVAKIAES
                     CGIQAISVHGRTRACGFAGNAEYDTIAAVKSEVGIPVIANGDIDSPEKAREVLSRTRA
                     DAIMIGRAAQGRPWIFREIEHFLVSGEHLPAPEVGEIHGVLVRHLRALYALYGEQSGV
                     RIARKHISWYASKLAGAATFRQTMNRLETAREQLAAVDRFFTELAGLGERLTYIEELA
                     A"
     gene            1105..1338
                     /locus_tag="VE030_01635"
     CDS             1105..1338
                     /locus_tag="VE030_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZF22146.1"
                     /translation="MNDNEIARCVRKAMDGYFRDLDGEKPCAIYNMVISSVEKPLLEL
                     VLHRVAGNQTQAAQLLGLNRNTLRKKMKEYGIK"
     gene            1407..2966
                     /gene="purH"
                     /locus_tag="VE030_01640"
     CDS             1407..2966
                     /gene="purH"
                     /locus_tag="VE030_01640"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HZF22147.1"
                     /translation="MARRQALISLSDKTGAADFGRALRHFGFEILSSGGTARLLREAG
                     IRVTEIGDYTGFPEMLDGRVKTLHPKVHAGILARRDLPAHLEAMAKAGIPTIDLVAVN
                     LYPFVETISRADCTLEEAIENIDIGGPTMVRAAAKNWQHVTVVTDPADYPALIAEMES
                     ASGAISVETRFRLARKAFSHTAAYDGAISNYLTATDIHGKRSRFPDQFNSSFVKLQDL
                     RYGENPHQQAAFYRDLKPVPGALSNYVQRQGKELSYNNIADADAAWECVKTFDQPACV
                     IIKHANPCGVAVAASAAEAYRRAFKTDPTSAFGGIIAFNRELDEETAAAVSQQFAEVL
                     IAPRVLAGAAAVLAQKSNLRVLEVAADTGGNDQDTRRVGGGLLVQTPDAMNVQAGDLR
                     IVTRKHPTPQQLADLLFAWRVAKFVKSNAIVYCAEGMTLGVGAGQMSRVDSARVASMK
                     AENAGLSLKGSVVASDAFFPFRDGVDVVARAGAAAVIQPGGSVRDEEVIAAADEHGIA
                     MVFTGVRHFRH"
     gene            3147..4430
                     /gene="purD"
                     /locus_tag="VE030_01645"
     CDS             3147..4430
                     /gene="purD"
                     /locus_tag="VE030_01645"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011885576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HZF22148.1"
                     /translation="MNLLVVGSGGREHALAWRLAQSPRIGRIYVAPGNAGTAREDGLF
                     NAPITSIPALVEFARKESIALTVVGPEAPLSEGIVDAFHAAGLKIFGPTRQVAQLESS
                     KDFAKSFMVANGIPTAAYATFSDARAAREYVRRQGAPIVIKADGLAAGKGVVVAMTLE
                     EAYAAIDMMLVDNRMGAAGARVVIEEFLEGEEASFIVMVDGKHVLPLASSQDHKRLLD
                     GDHGPNTGGMGAYSPAPVLTPTVHARIMREVVQPVVAGMDKRGTPYSGFLYAGLMIRP
                     NGDSRVLEFNCRMGDPETQPIMMRLKSDLFTLIEHATNGTLKNIEVEWDRRVALGVVM
                     AAAGYPDNPRKGDEITGLPRAADDFHVFHAGTALAGGKVVVNGGRVLCVTALGDTVRV
                     AQRRAYEIAEGIQFAGRQMRRDIGFRAIGAKKSTL"
     gene            4437..5354
                     /gene="hemF"
                     /locus_tag="VE030_01650"
     CDS             4437..5354
                     /gene="hemF"
                     /locus_tag="VE030_01650"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HZF22149.1"
                     /translation="MEIETIREYLTGLQDWIVGRLEAMDGNAFRRDSWDRPDGGGGVS
                     CLIEEGNLFERGGVNFSHVTGSSLPPPASVSRSDLAGRSFQAMGVSLVLHPRNPYVPT
                     VHMNVRFLVAEKGEADPVWWFGGGMDLTPYYGFEEDAVHFHQTCQAALDPFGAEAHPK
                     FKKWCDTYFFLKHRQEPRGIGGVFFDDLSEPDFDTCFALTRSVGDHFVDAYVPIAERR
                     KDLPYGERERDFQAYRRGRYVEFNLVYDRGTLFGLQSGGRTESILMSLPPMVKWRYDW
                     KPEPGSAEAELYEKFLVARDWVQVLSSKP"
     gene            complement(5344..6675)
                     /locus_tag="VE030_01655"
     CDS             complement(5344..6675)
                     /locus_tag="VE030_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZF22150.1"
                     /translation="MHDYAAHKARLAEQLKAGTGDLALRKDTSNLFRDRAQAAAQRLD
                     VRDFNHVLEVNSQEDWVDAEGMTSYEELVEATLRHGSMPAVVPQLKTITLGGALAGVG
                     IEATSFRHGLVHDTVLEMEVLAGDGRIYLCSAANEHRDLFFGFPNSYGTLGYALRVRA
                     KTLSVKKFVRIDHTGFRDPNAFFAALKEACSSPVDFVDGVVFGTTEMYLTTGSFTDEA
                     PFTSDYTYEHIYYRSIRNKARDYLAARDFLWRWDTDWFWCSRNLGAQNPLVRRLLGRS
                     RLNSRTYTEVMRWNSRWKLTHRFDGLLGLHRESVIQDVDIPLERAPEFLEFFRREIGI
                     LPVWICPIRAHNPQARFDLYPLVAGRTYVNFGFWDVVKSRVAHPGGHFNRLIERQVAE
                     LGGIKSLYSESYYPREEFWGIYGEAAYRRLKRKYDPQGRLRDLYDKCVLRA"
     gene            complement(6726..7946)
                     /locus_tag="VE030_01660"
     CDS             complement(6726..7946)
                     /locus_tag="VE030_01660"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HZF22151.1"
                     /translation="MATGLVRRKMAGLGDRAGIVFRVEFANGSHYQNGAGTPAFTIVI
                     HKRAAELRTALFGHMGFIESFFDGDIDIEGDLGAAFRVGMEGGFAHHSNPLNVIRNQW
                     HELRFSNRSIAQARKNARFHYGLGKDFYRYWLDLPSMMYTCAYWKEGTATVEEAQRNK
                     MDHVCRKIRLSRSETVVDIGCGFGGFLFHALEHYDAVPTGVNTTPEQVTAVRDEIRRR
                     GLDGRIEVREADFREVDRQYDKVVSIGVLEHAGRGQLHAVVRAHADFLKPGGLGMLHF
                     IGHVGVRDTEFYIRKHVFPGGWIPSLAETIAAMEECGLEVVDIENLRRHYALTLDAWG
                     ERFDRNWDKIRALDPAKFNEHFRRVWRTYLYACAEMFRSPRLTTHLFQIVFSKGNVSR
                     DNYPMSRAFLYDRL"
     gene            complement(8014..8724)
                     /locus_tag="VE030_01665"
     CDS             complement(8014..8724)
                     /locus_tag="VE030_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZF22152.1"
                     /translation="MNALLEVSGLKVAYGGINAVRGIDLNVGDGEMVALIGANGAGKT
                     TTLKAICGMLHHGGKVHYRGEDVSTLLSYQLVERGLALVPEGRGVFGRLTVHENLEMG
                     AYTHKNRAAVLADLDRTYSLFPRLAERRRQTAGTLSGGEQQMLAIGRALMSRPKLLLL
                     DEPSMGLAPMMVEKIFSTVRTISQEGVTVLLVEQNAKLALGASHRGYVMESGLINLAD
                     SARALLDNPQVRQAYLGE"
     gene            complement(8721..>9176)
                     /locus_tag="VE030_01670"
     CDS             complement(8721..>9176)
                     /locus_tag="VE030_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZF22153.1"
                     /translation="HVRTHAGALGAILRGRRTVAEESAIRRRARELLDYVGIARHSQA
                     LSKNLSYGDQRRLEIARALATEPKLLALDEPAAGMNATETADLRRLLIKIRDDAVTVL
                     LIEHDVKLVMGLCDRVAVLDYGKKIAEGEPVAVKNDPEVIRAYLGGALP"
BASE COUNT         1744 a         2904 c         2853 g         1675 t
ORIGIN      
        1 gcttgtaacc aaggttgtaa caacgggtag caattgcgcg ccctcaacac cgcatccttt
       61 acgctaaaat ggcgctcctc tttcggtgcc cgcataaatg cacatcggtc cgcacgttct
      121 caaaaacaat ctgatcgtcg cacccatggc gggtgtgacc gatcggccgt tccgccagtt
      181 gtgcaaggcg atgggtgcgg gaatggcggt ctcggagatg attgccgcga atccacaact
      241 gtggaacacg gcgaaatccc agcggcgttc cgatcatcgg ggtgaagttg atccgatttc
      301 ggtgcagatc gccggtgctg acccgcagat gatggccgat gcggcgcgct acaacgtcca
      361 gcgcggggcg cagatcgtcg acatcaacat gggctgcccg gctaaaaaag tctgcaacgt
      421 cgccgccggc tcggcgttgc tgcgcgacga acagttggtc ggaaaaattc tcgattcggt
      481 ggtcgcggcg gtcgaagtac cggtgacgct caaaatccgg accggctggg acgcgcacaa
      541 caggaatgca gtgcgagtgg caaaaatagc ggaatcctgc ggcatccagg cgatctcggt
      601 gcacggccgc acccgtgcct gcggttttgc cggcaatgcc gaatacgaca cgatcgccgc
      661 ggtaaaatct gaagtcggca tcccggtcat cgccaacggc gacatcgatt cacccgaaaa
      721 ggcgcgcgaa gttctttccc gcaccagggc cgacgcgatc atgatcggcc gtgccgcgca
      781 gggccgcccg tggatcttcc gcgagatcga gcacttcctc gtcagcggcg aacacctgcc
      841 tgcgcctgaa gtcggggaaa ttcacggcgt gctggtacgg catttgcgtg ctctgtacgc
      901 gctgtatgga gagcagtccg gcgtgcgcat cgcccgcaag cacatttcct ggtacgccag
      961 caagcttgcc ggtgcggcca cgttccgtca gaccatgaat cggctggaga ctgcacgcga
     1021 gcagctggcc gcggtggatc gctttttcac cgagcttgcc gggttgggag aacgcctgac
     1081 ctatatcgag gaacttgcag catgatgaac gacaacgaaa ttgcccgctg cgtgcgcaag
     1141 gcaatggacg gctatttcag ggatctggac ggggaaaaac cctgcgccat ttacaacatg
     1201 gtgatcagct cggtggaaaa gccgttgctc gagctggtgc tgcaccgggt cgccggcaac
     1261 cagacccagg ccgcgcagtt gcttgggctg aaccgcaata cgctgcgcaa gaaaatgaag
     1321 gagtacggca tcaagtagcg cgccgttgcc ccgcatcaac ccgcccgcgc gatcaacgcg
     1381 ggcggtttta tttttctggt cccgccatgg ctcgtagaca agccctgatc agcctttccg
     1441 acaagaccgg cgcagcggat ttcggccgcg cgctccggca cttcggattc gaaatcctgt
     1501 ccagcggcgg taccgccagg ctgctgcgcg aggccgggat cagggtcacc gagatcggcg
     1561 attacaccgg cttccccgag atgctcgatg gccgggtgaa gaccctgcat cccaaggtgc
     1621 acgccggcat cctcgcgcgc cgcgaccttc cggcgcacct cgaggcgatg gcaaaggctg
     1681 gcatcccgac catagacctg gtggcggtga atttgtatcc cttcgtggaa accatctcga
     1741 gggccgattg cactctcgag gaggcaatcg agaatatcga tatcggcggg cccacgatgg
     1801 tacgtgcggc agcgaagaac tggcagcacg tcaccgtggt caccgatccg gcggactacc
     1861 cggcgctgat tgcggaaatg gagtccgcca gcggcgcgat cagcgtggag acgcgcttcc
     1921 gcctggcgcg caaggctttt tctcacaccg cggcctacga cggcgcgatc agcaactacc
     1981 tcactgccac cgacattcac ggcaagcgca gccgattccc cgatcagttc aattcgagtt
     2041 tcgtcaaact ccaggacttg cgctacggcg agaatccgca ccagcaggcg gcgttctacc
     2101 gtgacctgaa gccggtaccc ggcgcgctga gcaattatgt gcaacggcag ggcaaggaac
     2161 tctcctacaa caacatcgcc gatgccgacg cggcctggga atgcgtgaag accttcgacc
     2221 agccggcgtg cgtgattatc aagcacgcca atccctgcgg cgtggcggtc gccgcgagcg
     2281 cagccgaggc ctatcgccgg gcgttcaaaa ccgatccgac ctcggcattc ggcggaatca
     2341 ttgcgttcaa ccgcgaactg gacgaggaaa ccgcggcggc ggtgtcgcag cagtttgccg
     2401 aagtattgat tgcaccgcgg gtgctggcgg gggccgcggc ggtgctggcg cagaagagca
     2461 acctgcgggt gctggaagtc gcagccgata ccggaggcaa cgaccaagat accaggcgcg
     2521 tcggcggtgg cctgctggtg caaacgcccg acgcgatgaa cgtccaggcc ggcgacctgc
     2581 gcatcgtcac ccggaaacat ccgaccccgc agcagcttgc cgacctgttg ttcgcgtggc
     2641 gcgttgccaa gtttgtcaag tccaacgcta tcgtgtactg cgccgaaggc atgacgctgg
     2701 gcgttggcgc ggggcagatg agccgggtgg atagcgcgcg cgtcgcctcg atgaaagcgg
     2761 agaatgccgg cctgtcgctc aagggttcgg tggtcgcatc ggatgcattt ttcccgttcc
     2821 gcgacggggt ggacgtcgtt gccagggccg gggcggcggc ggtcatccag ccgggcggta
     2881 gcgtacgcga cgaagaagtc attgccgccg ccgacgaaca cggcattgcc atggttttca
     2941 ccggcgtgcg ccatttccgg cactagcatt caccacagag gcacagagac acagagacac
     3001 agaggaagca aaggaagaca aaacgcatgc ggcataaggg agcatgacgg gaatccagag
     3061 ccccttgccc cctcttggta tcttggtgta agcattcatt ttggattttc tccgtgcctc
     3121 tgtgtctctg tggttaattc agacccatga acttactggt agtcggtagc ggcggtcggg
     3181 agcacgcact cgcctggcgg ctggctcagt cgccgcgcat cggacggatt tacgtcgcgc
     3241 cgggcaacgc cggtacggcg cgcgaggacg ggttgttcaa cgcgccgatc acctcgatcc
     3301 cggcactggt ggaatttgcc cgcaaagagt cgatcgcgct caccgtggtc ggtcccgagg
     3361 caccgctctc ggaaggcatc gtcgatgctt ttcacgccgc ggggttgaag atcttcgggc
     3421 caacgcggca ggtggcgcag ctcgaatcgt cgaaggactt tgccaaatcc ttcatggttg
     3481 cgaacggtat tccgacggcg gcgtacgcca ctttctccga cgcaagagcg gcgcgcgaat
     3541 acgtcaggcg gcaaggcgca ccgatcgtca tcaaggccga cggactcgcc gccggcaagg
     3601 gcgtggtggt ggcaatgacg ctcgaggaag cttacgccgc catcgacatg atgctggtcg
     3661 acaacaggat gggggccgca ggcgcgcgcg tggtcatcga ggagtttctg gaaggagagg
     3721 aagccagctt catcgtgatg gtggacggca agcacgtcct gccgctggcg tcgagccagg
     3781 atcacaagcg tctgctcgac ggcgaccatg gcccgaatac cggcggaatg ggcgcctact
     3841 cgccggcgcc ggtgctgacc ccgaccgtcc acgcacggat catgcgtgaa gtcgtccagc
     3901 cggtggttgc cgggatggat aaacggggca cgccgtacag cggatttctt tacgccggcc
     3961 tgatgatccg tccgaacggc gacagcaggg tgctcgagtt caactgccgg atgggcgatc
     4021 cggagaccca gccgatcatg atgcggctga aaagcgacct gttcacgctt atcgagcacg
     4081 ccaccaacgg tacactcaag aacatcgaag tcgagtggga ccgccgggtc gcgctcggcg
     4141 tagtgatggc cgcagccggc tatccggaca atccgcgcaa gggcgacgag ataaccggcc
     4201 tgccgcgcgc tgccgatgat ttccacgtat ttcacgccgg gaccgcgctg gcgggcggca
     4261 aggtcgtcgt caacggcggg cgggtgctgt gcgtgaccgc gcttggcgac acggtgcggg
     4321 tggcgcagcg gcgcgcttac gaaatcgcgg agggcatcca gttcgcaggg cggcagatgc
     4381 gccgcgacat cggcttccgc gccatcggcg ccaagaaaag cacgctatag gccagcatgg
     4441 aaatcgagac catcagggag tatctgaccg gcctgcagga ctggattgtc ggccgcctcg
     4501 aggcaatgga cggcaacgcc ttccgccgcg attcatggga tcgtcctgac ggcggcggcg
     4561 gggtgagttg cctcatcgag gagggaaacc tgttcgagcg cggcggggtc aacttctctc
     4621 acgtcaccgg gtcgtcgctt ccgcctccgg ccagcgtgtc gcgttccgat cttgccggac
     4681 ggtccttcca ggcaatgggc gtgtcgctgg tgttgcaccc gcgcaaccct tatgtcccca
     4741 ctgttcacat gaacgtgcgt ttcttggtgg ccgagaaggg cgaggccgat ccggtgtggt
     4801 ggttcggggg cgggatggat ctgacgccgt actacggatt cgaggaggac gcggtccact
     4861 tccaccagac ctgccaagcc gcgctcgacc cgttcggagc cgaagcgcat ccgaaattca
     4921 agaagtggtg cgacacgtat tttttcctca agcatcgcca ggaaccgcgc gggatcggcg
     4981 gcgttttttt cgacgacctg tccgaaccgg atttcgatac ctgttttgct ctcacccgca
     5041 gcgtcggcga ccatttcgtc gacgcctacg tcccgattgc cgaacgccgc aaggatctgc
     5101 cttacggcga acgcgaacgc gactttcagg cctatcggcg cgggcgttac gtcgagttca
     5161 acctggtcta cgaccgcggc acgctgttcg gactgcaatc cggcgggcgc accgaatcca
     5221 tcctgatgtc gctgccgcca atggtgaagt ggcgctacga ctggaagccc gagcccggca
     5281 gcgccgaggc agagttgtac gagaagttcc tggtcgcgcg cgattgggtc caggttctaa
     5341 gctctaagcc ctgagtacgc acttgtcgta caggtcgcgc agcctgcctt gcggatcgta
     5401 cttgcgtttc aggcgccggt aggctgcttc gccgtagatc ccccagaact cctcgcgcgg
     5461 atagtagctt tcggaataaa gcgacttgat tccgccgagt tcggcgacct ggcgttcgat
     5521 gaggcggttg aaatgcccgc ccgggtgggc gacccgagat ttcaccacgt cccagaaacc
     5581 gaaattgaca taggtcctgc ccgcgaccag cgggtacagg tcgaatcgcg cctgcggatt
     5641 gtgcgcccgg atcggacaga tccagaccgg caggatgccg atctcgcgcc ggaagaattc
     5701 gaggaattcg ggcgcgcgct cgagcgggat atccacgtcc tggatcactg actcgcgatg
     5761 tagccccaat aacccgtcga accggtgagt cagcttccag cggctgttcc agcgcatgac
     5821 ctcggtgtag gtgcgcgagt tgagccgcga cctgccgagc agcctgcgta ccagcgggtt
     5881 ctgcgcgccg agattcctgg agcaccagaa ccagtcggtg tcccagcgcc acaggaaatc
     5941 gcgcgccgcg aggtaatctc tagccttgtt gcggatcgag cggtagtaga tatgctcgta
     6001 agtgtagtcg ctcgtaaatg gcgcttcgtc cgtgaagctt cccgtggtca gatacatctc
     6061 agtggtgcca aataccacgc catcgacaaa atcgaccggc gatgagcagg cctctttgag
     6121 cgccgcaaaa aaggcgttcg ggtcgcgaaa gccggtatga tcaatgcgca cgaacttctt
     6181 taccgacagc gtctttgcac gcaccctcaa cgcgtagccc agcgtgccgt aggaattggg
     6241 aaaaccgaag aacaggtcgc gatgctcgtt cgccgccgaa cacagataga tcctgccgtc
     6301 gccggccagg acttccatct ccagcaccgt gtcgtgcacc aggccatggc ggaacgaagt
     6361 ggcttcgatg cccactccgg cgagcgcgcc accgagggtg atggtcttca gttgcggcac
     6421 cacggccggc atcgagccgt ggcgcagtgt ggcctcgacc aactcctcgt agctggtcat
     6481 gccttcggca tccacccagt cttcctgcga attgacttcc agcacgtgat tgaaatcgcg
     6541 cacgtcgagg cgttgcgccg cggcctgcgc acggtcgcgg aacagattgg aggtgtcctt
     6601 gcggagcgcg aggtcgcctg tgccggcctt gagctgttcg gctagtctcg ccttgtgcgc
     6661 tgcgtagtcg tgcatgcgcc gctccggaga aagatggctc gatgccgcgc cggccgcagg
     6721 cacgctcaga gccggtcgta gagaaaagcg cggctcatcg gataattgtc cctcgagaca
     6781 ttacccttgc tgaacacgat ctggaacagg tgcgtggtca ggcgtggcga gcgaaacatt
     6841 tccgcacagg catacagata ggtgcgccat acccggcgaa agtgctcgtt gaatttcgcc
     6901 ggatcgagcg cccggatctt gtcccagttg cggtcgaagc gctcgcccca ggcatccagc
     6961 gtcaatgcat agtgacggcg caggttctcg atatcgacga cttcgagccc gcactcctcc
     7021 atggcggcga tggtttccgc caggctcggg atccagccgc cgggaaacac gtgcttgcgg
     7081 atgtagaact cggtgtcgcg cacgccaaca tggccgatga agtgcagcat gccgaggccg
     7141 ccgggtttga ggaaatccgc gtgggccctg acaaccgcgt gcaactggcc gcgcccggca
     7201 tgctcgagca ctccgatcga gaccaccttg tcgtactgcc ggtccacctc gcggaagtcg
     7261 gcctcgcgca cttcgatcct gccgtccagc ccgcggcggc ggatctcgtc gcgcaccgcc
     7321 gtgacttgct cgggagtagt gttgacaccg gtcggcaccg catcgtagtg ctccagcgca
     7381 tgaaacagga agccgccgaa tccgcaaccg atgtccacca ccgtctcgct gcgcgacagc
     7441 cggatcttgc ggcagacatg atccattttg ttgcgttgcg cctcttcgac cgtcgcggta
     7501 ccttccttcc agtaggcgca ggtgtacatc atcgacggca aatcaagcca atagcgatag
     7561 aaatccttgc ccaggccata gtggaaacgc gcgttctttc tggcttgcgc gatcgagcgg
     7621 ttggagaagc gcaactcgtg ccattggttg cgaatgacgt tcagaggatt cgagtgatgc
     7681 gcgaagccgc cttccatccc cacccggaat gccgccccca gatcgccttc gatatcgatg
     7741 tcgccgtcga aaaaggactc gataaatccc atatgcccga acaacgcggt gcgcagctcg
     7801 gccgcacgct tgtgaatcac aatggtgaac gccggcgtgc cggcgccatt ctggtagtgc
     7861 gagccgttcg caaattcgac ccgaaagaca ataccggcac gatcacccag tcccgccatc
     7921 ttgcgcctta ccagtccggt cgccatttct ttttcacctc gtcgcaatcc tgttgccccg
     7981 ccccgatcgg gacaccgacg gttgcgcctc cgcctactct cccaggtatg cctgccgcac
     8041 ctgcgggttg tcgagcaaag ccctggcgct gtcggccaag ttaatcagcc cgctttccat
     8101 cacgtaaccc cgatgactcg ctcccagtgc aagctttgcg ttctgctcga ccagcagcac
     8161 cgtaacgcct tcctgcgata tggtgcgcac ggtcgagaaa atcttctcca ccatcatcgg
     8221 cgccagtccc atgctcggtt catcgagcaa cagcagtttc ggccggctca tcaacgcgcg
     8281 cccgatggcg agcatctgct gctcgccccc cgagagcgtg cccgcggtct gcctccggcg
     8341 ttcggcaagc cgcgggaaca gcgagtaagt gcgatcgaga tcggcgagaa ccgcggcgcg
     8401 gtttttatgc gtgtacgcac ccatttccag gttctcgtgc accgtcagcc ggccgaacac
     8461 gccgcgccct tccggcacca gcgccagccc gcgctcgacc agctggtagg acaacaatgt
     8521 actgacatcc tccccgcggt agtgcacctt gcccccatga tgcagcatcc cgcagatcgc
     8581 cttcaacgtt gtcgtcttgc ccgctccatt ggcgccgatc aacgccacca tctcgccatc
     8641 gcccacgttc aggtcgatac ccctgaccgc gttgatgccg ccgtaggcca ccttcaaccc
     8701 ggaaacctcc agaagggcgt tcaaggcaac gcgcctccga gataggcgcg aatcacttcc
     8761 ggatcgttct tcaccgctac cggctcgcct tcggcgattt ttttgccgta gtcgagcacc
     8821 gccacccggt cgcacaaacc catcactagc ttgacgtcgt gctcgatcag caggacggtc
     8881 accgcgtcgt cgcggatctt gatgagcaga cggcgcagat cggcggtttc ggtggcattc
     8941 atgccggcgg ccggctcgtc cagcgccagc agcttcggct cggtggcaag cgcccgcgcg
     9001 atctccagac gccgctggtc cccgtaggac agattcttcg acagcgcctg cgagtggcgc
     9061 gcgatgccga catagtcgag caactcgcgc gcgcggcggc ggatcgccga ttcctccgcc
     9121 acggtgcgcc ggccgcgcag gattgcaccg agagcccctg catgggtacg cacgtg
//
LOCUS       DASQOP010000043         2646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1627539, whole genome shotgun sequence.
ACCESSION   DASQOP010000043 DASQOP010000000
VERSION     DASQOP010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1627539"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..>2646)
                     /locus_tag="VE030_01675"
     CDS             complement(69..>2646)
                     /locus_tag="VE030_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004350825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZF22154.1"
                     /translation="VASRGLTAGDVVRAMRDQNIQVSAGQLGAEPIKGGSDFLIAINA
                     QGRLQSAGEFGNIVLKTGANGEVVRLADVARIELGASDYTLRGALDGGYTTAIGVFQA
                     PGANMLAVRDAVIAKMDELAKQFPPGMTYRSDYDSTIFVRDSIKAVVHTLGEAILLVV
                     LVVILFLQTWRASIIPLIAVPVSVVGTFAVLYLLGFSINTLTLFGLVLAIGIVVDDAI
                     VVVENVERNIEEGRTPLQAAHQAMKEVSGPIVAIALVLCAVFVPMAFLTGVTGTFYKQ
                     FAVTIAISTVISAINSLTLSPALAAKLLHAHGAPKDAISRGIERAFGWVFGPFNRFFK
                     ASSDKYHGSVSRILKRRGVAFAVYAVLLAGTGLMFNDVPRGFIPTQDKLYLIAGVKLP
                     EGASIGRSDAVVKQMVTMAQETEGVAHVVSFAGLNPTQFTNTPNYAIAFVILKKFDER
                     HISAKQIADTMQAKLNTIQEGMAFVMMPPPILGLGNGAGYSLFVEDRAGSGYGVLQQG
                     VGALQGALMKEPGMGFPFSGYQSNVPQLDVDVDRLKAKTQGVALSDLFETLQVYLGST
                     YVNDFNLFGRTWRVYAQAEGDYRKKVEDIANLKTRNAKGEMVPVGSMVNIKQSYGPDP
                     VIRYNGYPAADFVGEANPALLSSAQAMDKITEIAGKVLPNGMSFEWTDLSFQEATQGT
                     ASLLVFPVAILLAFLVLAALYESLLLPLAVILIVPMCMLSALAGVKLFGGDNNTFVQV
                     GLVVLMGLACKNAILIVEFARELEFQGKGVVEAALEACRLRLRPIVMTSIAFIAGTVP
                     LVLSHGAGAEVRSATGITVFAGMIGVTLFGLLLTPVFYVGLRKLSGRKLVQHGEGTMA
                     HD"
BASE COUNT          516 a          842 c          832 g          456 t
ORIGIN      
        1 tgcgtttggc cgcatcgatg agcgatttgc tttgcacaat cggtacacga atccgcaccg
       61 accgagcttt agtcatgcgc catggtgccc tcaccatgct ggaccagctt gcggccagac
      121 agcttgcgca ggccgacgta gaacaccggc gtgaggagca ggccgaatag ggtcacgccg
      181 atcatcccgg cgaacacggt gatgccggtg gccgagcgca cctcggcgcc tgcgccatgc
      241 gagagcacca gcggaacggt gccggcgatg aaggctatcg aggtcattac gatcgggcga
      301 aggcgcaggc ggcaggcttc gagcgcggct tcgacgacgc ccttgccctg gaattccagc
      361 tcgcgcgcga actccacgat caggatcgcg ttcttgcacg ccaggcccat cagtaccacg
      421 aggccgacct gcacgaaggt gttgttgtcg ccgccgaaca gcttgacacc ggccagcgcc
      481 gaaagcatgc acatcggcac gatcaagatc accgcgagcg gcagcagcaa actctcgtac
      541 agcgcagcga gcacgaggaa ggccagcagg atcgcgaccg ggaacacaag caacgaggcc
      601 gtgccctggg tcgcttcctg aaaactcagg tcggtccact caaagctcat gccgttcggc
      661 agcaccttgc cggcgatctc ggtgatcttg tccatcgcct gcgcggagga cagcagagcc
      721 gggttggcct cgccgacgaa atcggcggcc ggatagccgt tgtagcggat caccggatcg
      781 gggccgtagc tctgcttgat gttcaccatc gagccgaccg gcaccatctc gcccttcgcg
      841 tttcgcgtct tcaggttggc aatgtcctcg acttttttgc ggtagtcgcc ctcggcctgc
      901 gcatacacac gccatgtgcg gccaaaaaga ttgaaatcgt tgacgtaggt cgagccgaga
      961 tagacctgca aggtctcgaa caagtcgctc agcgccacgc cctgggtctt ggccttgagg
     1021 cggtcgacgt ccacgtcgag ctgcggcacg ttggattgat agcccgagaa cgggaagccc
     1081 atacccggct ccttcattaa cgcaccttgc agcgcaccca cgccttgctg cagcacgccg
     1141 tagccgctgc cggcgcgatc ctcgacgaat agcgaatagc cagcgccgtt gccaaggccg
     1201 aggatcggcg gcggcatcat cacgaaagcc atgccttcct ggatcgtgtt gagcttggcc
     1261 tgcatcgtgt cggcgatctg cttggcgctg atgtggcgct cgtcgaactt cttcaggatt
     1321 acgaaagcaa tagcgtagtt gggagtgttg gtgaattggg tcgggttcag gccggcgaac
     1381 gaaactacgt gagcaacgcc ttcggtctcc tgggccattg tgaccatttg cttcaccacc
     1441 gcgtcggagc ggccgatcga ggcaccctcc ggcaatttca cgcccgcgat caggtagagt
     1501 ttgtcctggg tcgggataaa gccgcgcggc acgtcgttga acatcagacc cgtgcccgcg
     1561 agcagcaccg cgtagaccgc gaacgccacg ccgcggcgct tgaggatgcg cgacacggag
     1621 ccatgatact tgtccgaact cgccttgaag aagcgattga atgggccgaa gacccagccg
     1681 aacgcgcgct cgatgccacg cgagatggcg tccttgggcg cgccgtgcgc gtgcagcagc
     1741 ttggcagcga gcgccggcga caaggtcaac gagttgatcg ccgagatcac cgtcgagatc
     1801 gcgatggtta ccgcgaactg cttgtagaag gtaccggtga cgccggtcag gaaggccatc
     1861 ggcacgaaca ccgcacacag caccagcgcg atcgccacga ttggcccgga aacctctttc
     1921 atagcctggt gcgcggcttg cagtggcgtg cgcccttcct cgatattgcg ctcgacgttt
     1981 tcgaccacga cgatcgcgtc gtcgacgacg atgccgatcg ccaggacgag gccgaacaag
     2041 gtcagtgtgt tgattgagaa gccgagcaga tacagcacag cgaaggtgcc gactaccgaa
     2101 accggcaccg cgatcagcgg aatgatcgag gcgcgccagg tctgcaggaa caggataacc
     2161 accagcacga ccaacaggat cgcctcgccc agcgtgtgca ctacagcctt gatcgaatcg
     2221 cgcacgaaga tcgtgctgtc gtagtccgag cggtaggtca tgcccggggg gaattgcttg
     2281 gccagctcgt ccatcttcgc aatcaccgca tcgcgtacgg ccagcatgtt ggcgcccggc
     2341 gcctggaaca cgccgatcgc cgtggtgtac ccgccgtcga gcgcgccgcg caaggtgtag
     2401 tcgctggcgc cgagttcgat gcgcgccacg tcggcaaggc gcacgacctc gccgttggcg
     2461 ccggttttga gcacgatgtt gccgaactcc ccggccgatt gcagtcggcc ctgcgcattg
     2521 atggcgatca ggaaatcgct accgcccttg atcggttcgg cgccaagctg gccggccgag
     2581 acctggatgt tctgatcccg catcgcgcgg accacgtcgc ccgcggtcag gccgcgcgag
     2641 gcgacc
//
LOCUS       DASQOP010000044         3418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2635353, whole genome shotgun sequence.
ACCESSION   DASQOP010000044 DASQOP010000000
VERSION     DASQOP010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2635353"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VE030_01680"
     CDS             complement(<1..762)
                     /locus_tag="VE030_01680"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZF22155.1"
                     /translation="MILSEISIKRPVLAIVMSLVIVLMGFLSYSRMAVREYPNIDPPV
                     VSVRTVYKGATAQVIESAVTQPLEDSLSGIEGIKTIKSNSREEVSQITVTFLTSREVD
                     AAAADVRDRVSRIRKQLPATIDEPVVSKIEADAQAIIWIAVSSDRQSPMEITDFADRY
                     LTDPLKALPGVSSVIIGGERKYSMRVWIDRERLAAQGLTVHDIETALINQNVESPGGR
                     IESTQREFTVQPRTDLRSPEDFNNMIISTAKGYPVR"
     gene            complement(790..1947)
                     /locus_tag="VE030_01685"
     CDS             complement(790..1947)
                     /locus_tag="VE030_01685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZF22156.1"
                     /translation="MHTPIIRNVAILTALVTAALIAGCGSKNDATAGQKADAAKPAPQ
                     QPATPAAQPQGLPVRAEAVKVAKVSDDVSAVGSLMAEESVIIRPEIDGRIVGLHFQEG
                     QAASAGTRLVTIDSTEYEAQAAAVRADLKTEEQRLVRTKELHQQNFISKDALDVQVGA
                     VDRLKARLAEAESRVAKTVIRAPFSGIVGLRQISPGAYVKAGTDIVRIENVSSIKVDF
                     RIPENYLSKIRPNQEILVRLDAYPGEEFRGRVYAVEPVVEERTRTIAMRARIPNRGFK
                     LKPGMFVRVAVTMENRPNAIVIPEQAIWPQGKDSFVFRVVDGKAALTKVEIGNRQPGS
                     VEILKGLGANDMVVTDGQIKLRDGAPVAVMAAPPGAAPGAPASGTAVPAKS"
     gene            2151..3083
                     /locus_tag="VE030_01690"
     CDS             2151..3083
                     /locus_tag="VE030_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZF22157.1"
                     /translation="MRISLDALLVLDSIARNGSFAAAGEELHRVPSAITYTIQKLEQD
                     LEVKLFDRSGHRAKLTEAGDELLREGRHLLRSATDLECRVKRVATGWETELRIAVDDV
                     IPTERLFPLLKDFYREASGTRIKLTAEILGGCWDALMTGRADLVIGAPGEAPPEGTLS
                     VEPMGQFEFIFVAAPDHPLAAAPEPLSSQDIMQFRAVSAADTSRSLPPRTSGLLDGQD
                     ALTVPTMRAKMAAHIAGLGAGYLPLAWAAPHIAGGRLVRKQVEGTKSLTNVYLAWHPR
                     QAGKALKWFLERLRDKTVQQYILGESGNYPSTGG"
     gene            complement(3088..>3418)
                     /locus_tag="VE030_01695"
     CDS             complement(3088..>3418)
                     /locus_tag="VE030_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22158.1"
                     /translation="DSDAATFAALANDVLGKHPAVAFAQVYRNSRGLFDMFGLHGTAT
                     AIFRGRVGMYLGTGLAAADQLVRLLDGVAALNMERVRAEIEQETSARESLAVHRVCPA
                     TRRGKLE"
BASE COUNT          607 a         1094 c         1053 g          664 t
ORIGIN      
        1 acggacgggg taacctttcg ccgtcgagat gatcatgttg ttgaaatctt ccggcgatcg
       61 cagatcggtg cgaggctgca ccgtgaactc gcgttgcgtg ctttcgatgc gcccgccggg
      121 agattccaca ttctggttga tgagcgcggt ctcgatgtca tggacggtca gtccctgcgc
      181 ggccaggcgt tcgcgatcaa tccatacccg catcgaatac ttgcgctcgc cgccgatgat
      241 cactgacgac acgcccggca gtgccttgag cgggtcggtc agataacggt ccgcgaaatc
      301 ggtaatctcc atcggcgact ggcgatcgct ggacacggca atccagatga tcgcctgcgc
      361 gtccgcctcg atctttgaaa ccaccggctc atcgattgtg gccggcagtt gcttgcgaat
      421 gcgggatacg cggtcgcgca cgtcggccgc cgccgcatcc acctcgcggc tggtcaggaa
      481 ggtgaccgtg atctggctca cttcctcgcg gctgttggat ttgatggtct tgatgccctc
      541 gatgcccgag agcgaatcct caaggggttg cgtgaccgcg ctttcgatga cctgcgcagt
      601 cgcgcccttg tagacggtgc gcacggaaac gaccggcgga tcgatgttcg ggtactcgcg
      661 aaccgccatg cgcgaatacg acaggaaacc catgagcacg atgaccaggc tcatgacgat
      721 ggccaaaacc ggccgcttga tcgagatctc ggagagaatc atggtgccgc tcctgacagg
      781 gttgccggac taactcttcg cgggaacggc cgtacccgag gcgggcgcgc ccggcgctgc
      841 gccgggcggc gcagccatca ccgccacggg agcgccgtcg cgcagcttga tctggccatc
      901 ggtgacaacc atgtcgttgg cgccgagtcc cttgaggatt tccaccgatc ccggctgccg
      961 gttgccaatt tccaccttgg taagcgcggc cttgccgtcg accacgcgga aaacgaaact
     1021 atcctttccc tgcggccaga tcgcttgctc ggggatgacg atcgcgttgg ggcgattttc
     1081 catggtcacg gccacccgga cgaacatgcc cggcttcagc ttgaagcccc tgttgggaat
     1141 gcgggcgcgc attgcgatag tacgagtcct ctcttcgacc acgggctcga ctgcgtatac
     1201 ccggcccctg aattcctcgc cgggataggc atccagccgc accaggattt cctggttggg
     1261 gcggatcttc gaaaggtaat tctcggggat gcggaaatcc accttgatgg aactcacgtt
     1321 ctcgatccgg actatatccg tgccggcctt gacgtaggca cccgggctga tctgccgcaa
     1381 accgacaatg ccggagaacg gcgcccgaat cacggtcttg gccacgcgcg attccgcttc
     1441 tgcgaggcgt gccttgagac gatccaccgc gccaacttga acgtcgagcg cgtccttgct
     1501 gatgaaattt tgctgatgca gttccttggt gcgaaccagg cgctgttctt cggtcttgag
     1561 atcggcacgt accgcggccg cctgtgcctc gtactcggtg ctgtcgatgg ttaccagccg
     1621 cgtgccggcg gacgccgcct gaccttcctg gaagtgaagg ccgacgatgc gcccgtcgat
     1681 ttccggccgg atgatgaccg attcctcagc catcaaagag cccactgcgg aaacgtcatc
     1741 ggaaaccttt gcaaccttta cggcttccgc cctgacggga agtccctgcg gctgggccgc
     1801 gggagttgcc ggctgttgcg gcgcgggttt tgccgcgtcc gccttctggc cggcagtagc
     1861 atcgttcttc gagccgcagc ccgcgatcag cgctgcggtg accagcgccg tgagaatggc
     1921 tacattacgg atgatcggcg tatgcacttg cttacctcta aactctggtc gagggcgcaa
     1981 aacggccggc ttcgactgat ggagaaaaaa tatcgccagc ggggactcct cgctggcaac
     2041 cggccgccat gcggccattt caaatacggg gcgcccgctg acggctttat tttgctgact
     2101 gacgagtcat ttattttcca acatcataac cgagttttat tgcctccacc atgagaatct
     2161 cgctcgacgc cctgctggtc ctggactcga ttgcgcgcaa cggcagtttc gccgcagcag
     2221 gcgaggaact gcaccgcgtt ccctcggcga tcacctacac catccagaag ctcgagcagg
     2281 acctggaggt gaagcttttc gatcgtagcg gccaccgtgc caaattgacc gaagctgggg
     2341 acgaactgct gcgcgaaggc cgccatctgc tgcgcagcgc caccgacctg gagtgccggg
     2401 tcaagcgggt cgccaccgga tgggaaaccg agttgcgcat cgcggtcgac gatgtcattc
     2461 ccaccgagcg gctgtttccg ctgctgaagg atttttaccg cgaagcctcc ggcacgcgca
     2521 tcaagctgac ggccgaaatt ctcggtggat gctgggacgc cttgatgacc ggccgtgccg
     2581 atctggtgat cggcgcgccc ggggaagcgc cgcccgaggg aacgctgtcg gtggaaccga
     2641 tggggcagtt cgagtttatc ttcgtggccg ccccggacca ccctctggcg gccgcgcccg
     2701 agccgctgtc ctcgcaggac atcatgcagt tccgcgcggt gtctgcggcg gatacctcgc
     2761 gcagcctgcc accgcgcacc tcgggattgc tggatgggca ggacgcgctc accgttccca
     2821 ccatgcgcgc gaagatggcc gcgcacatcg ccggcctggg cgcaggttac ctcccgcttg
     2881 cctgggccgc accgcacatt gccggcgggc ggctggtacg aaagcaggtg gagggaacga
     2941 agtctctgac caacgtgtat ctggcctggc acccgcgcca ggccggcaag gcgctcaaat
     3001 ggttcctcga gcgcctgcgc gacaagacag tccagcaata cattctgggc gagtcgggta
     3061 attatccgtc gaccgggggc tgacgtttta ttccaacttg ccgcgtctcg tcgccggaca
     3121 gacacgatgc accgccagcg attccctcgc cgatgtttcc tgttcgatct ccgcccgcac
     3181 acgttccatg ttcagcgcgg cgacgccatc gagcaaccgt accaactggt cggcagccgc
     3241 caggccggtc ccgagataca tgccgacacg cccgcggaag attgccgtgg cggtcccgtg
     3301 cagtccgaac atgtcgaaca gcccgcggct gttgcgatag acctgcgcga aagcaacggc
     3361 gggatgcttc ccgaggacat cattcgcaag cgcggcaaat gttgctgcgt cgctatcc
//
LOCUS       DASQOP010000045         5517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_217494, whole genome shotgun sequence.
ACCESSION   DASQOP010000045 DASQOP010000000
VERSION     DASQOP010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5517
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217494"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VE030_01700"
     CDS             complement(<1..304)
                     /locus_tag="VE030_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HZF22159.1"
                     /translation="MPRLHQRPAWKALEAHAHEVREVRVADLFAQDPARAQEFSIAAC
                     GLLFDYSKNPLSRQTRRLLVALAEDASLPAQITAMFEGKRINVTEKRAVLHTALRNR"
     gene            complement(460..993)
                     /locus_tag="VE030_01705"
     CDS             complement(460..993)
                     /locus_tag="VE030_01705"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HZF22160.1"
                     /translation="MSHVPEQLVATNKENLEAVIAFAGITAAGAEKLIELQLRTAKNV
                     FADAVKNAKALAGVKDVQELTEIQTSIAQPLADKITTYGRSVYAVIAETQAELNKFFE
                     ERIAETNKSFVTVLDQAVKNAPAGSDVAVAAVKSAMAAANQAYDAFSKATKQVAEATE
                     ATMTAAGSTVTTKKKAA"
     gene            1219..1968
                     /locus_tag="VE030_01710"
     CDS             1219..1968
                     /locus_tag="VE030_01710"
                     /inference="COORDINATES: protein motif:HMM:NF018917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HZF22161.1"
                     /translation="MEAVAKALAKAVGSLVHPVILLILLVPMVVAAAVWLGAAWAYWD
                     IWASGIQSLVVDHAAYSWTARWDLTKAAGVVAVVVIVILLTPAIIITALLIAAIFAMP
                     VLVSHVARRDYPQLERRRGGTVLGSAWNAFVAIGLSLVLWVVTIPLWLLGPIAAVLPL
                     LLSAYLNQRLFRYDALSEHASREEMQRIFAEARGKLFLLGLIAGVLYFIPPFNLIGPV
                     FAALAFVHLCLTELESLRSTESEIRGERLLR"
     gene            complement(2021..2350)
                     /locus_tag="VE030_01715"
     CDS             complement(2021..2350)
                     /locus_tag="VE030_01715"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HZF22162.1"
                     /translation="MRLSSINSPPDCLAKKYTGLVRNSKGLQVSIPSNIAEGHAREST
                     KEYLRYLLVSRGSMAELETQLLLCQKLQLLSKEDVEHALIVSHEIGRMIRGLQKSLTK
                     KLNKKSA"
     gene            complement(2446..3633)
                     /locus_tag="VE030_01720"
     CDS             complement(2446..3633)
                     /locus_tag="VE030_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase family protein"
                     /protein_id="HZF22163.1"
                     /translation="MNNGREVVVVSGVRTAIGDFGGSLKDFAPTELGARCVREALSRS
                     KVAPDEVGHVVFGNVIHTEAKDMYLARVCAINGGIPKEVPAVTLNRLCGSGLQAIVTA
                     AQIVALGDADVAVAGGVESMSRANYWLPSARWGSKMGDQVVMDSLIGALSDPFDTSHM
                     GVTAENVAAKYGISRETQDGFSVESHRRAANAIKNGWFKSQILPIEIKDRKGTKNFDT
                     DEHVRVETTMDSLGKLKPVFKKDGSVTPGNASGINDGAAAVVLMERKLAEKRGLKPMA
                     RLVAYGHAGVEPSLMGLGPIPAVQNAMKKAGLKVADLDVIESNEAFAAQACAVAKDLG
                     FDPKKTNPNGGAVALGHPVGATGCLISVKALYELQRIGGKYALVTMCIGGGQGIAAIY
                     ERM"
     gene            complement(3867..5045)
                     /locus_tag="VE030_01725"
     CDS             complement(3867..5045)
                     /locus_tag="VE030_01725"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008334339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HZF22164.1"
                     /translation="MDDVVIVAALRTAVGKFGGTISRIPASELGAKVITALFQKTGVK
                     PEMVSEVLLGQVLTAGVGQNPARQASIRAGVPAMVPAMTINKVCGSGLKATHLAAQAI
                     RAGDSEIVVAGGQESMSLAPHVLNGARDGLRMMDNKLTDTMIVDGLWDAYNQYHMGIT
                     AENVAKKWGVTRQEQDEFAAASQQKAEAAQKSGRFKDEIVPVIIPQRKGDPVVFDSDE
                     FVRHGTTAQSLAGLKPAFDKNGTVTAGNASGINDGAAAVMMMSAGKAKELGLKPLARI
                     KAYSSAGVDPSIMGIGPIPASKLCLKKAGWTPKDLDLLEINEAFAAQAIAVNRDMGWD
                     TAKVNVNGGAIAIGHPIGASGCRILVTLIHEMIKRDAKKGLASLCIGGGMGVALAIER
                     "
     gene            complement(5318..>5517)
                     /locus_tag="VE030_01730"
     CDS             complement(5318..>5517)
                     /locus_tag="VE030_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22165.1"
                     /translation="EYFGNILTERLEKGNRRTDCDVLIIQDGWDHVHKVGRAWEMIWE
                     GGRPGDSKETLRLYRLARSLS"
BASE COUNT          931 a         1687 c         1682 g         1217 t
ORIGIN      
        1 atcggttgcg caatgccgta tgcagcaccg cgcgcttctc ggtgacgttg atgcgcttgc
       61 cttcgaacat tgccgtgatc tgcgcgggca ggctggcgtc ctcggcgagc gccaccagca
      121 ggcggcgcgt ctgcctggac agcgggttct tcgagtaatc gaacagcagc ccgcaggcgg
      181 caatcgagaa ttcctgcgcg cgcgcgggat cctgcgcgaa caaatcggca acgcgcacct
      241 cgcgcacctc gtgggcatgg gcctctagtg ctttccaggc gggacgttgg tgcaggcggg
      301 gcatgggaaa ggggcgaggg gcgggggacg aggggcgagg gggcggatga aggaatctgg
      361 ttttaaccgg attctatagg acggccggga aataccgaac gggcaaaaca aagggcctcg
      421 ggatggctcc cgaggccctt caaaggaatc tggtgtggct taagcggctt ttttcttggt
      481 cgtgacggtg ctgcccgcgg cggtcatcgt tgcttcggtc gcttcggcga cttgcttggt
      541 agccttggag aaggcgtcgt aggcctggtt tgcagcagcc attgcggact tcactgcggc
      601 cacggcaaca tcggagccgg ccggcgcgtt cttgacggcc tggtccagca ccgttacgaa
      661 gctcttgttg gtttcggcga tgcgctcttc gaaaaacttg ttcagttcgg cctgggtttc
      721 ggcgatcact gcgtatacgc tgcggccgta ggtggtgatc ttgtcggcga gcggctgggc
      781 gatcgaggtc tggatttcag tcagttcctg cacatctttg acgcctgcga gcgctttggc
      841 gttcttgact gcatcggcga agacgttctt cgcggtccgc agttgcagct cgatcagctt
      901 ttccgcaccg gctgcggtga tgccggcgaa tgcgattacg gcttcaagat tttccttatt
      961 cgtggcgacc agttgttcgg gtacgtggct catagactct cctgggagac gaggtggacg
     1021 atttagggga tgcatactgc cgggggtttc gcgacgccgt ttagggtttt gttgcaccgc
     1081 aaaaactcga ccattctagc gatcccggat tggaagtcaa caagaaatcg tagttctact
     1141 atgcggcttc tgcgatccgc aagaaatgtg taatttaaca atcagttgaa tgaaattaca
     1201 gattttcgag gagcccctat ggaagcagtc gccaaggcgt tggcaaaggc cgtgggctct
     1261 cttgtgcacc ccgtcattct gctgattctc ctggtcccga tggtggtcgc ggcggcagtc
     1321 tggctggggg cggcatgggc gtactgggac atctgggcct cgggcatcca gtcgctggtg
     1381 gtggaccacg cggcgtattc ctggaccgcc cgctgggacc tcaccaaggc ggcgggggtg
     1441 gtcgccgtgg tcgtgatcgt gatcctgctc acgcccgcga tcatcatcac cgcgttgttg
     1501 atcgccgcga tattcgcgat gccagtgctg gtcagtcacg ttgcacggcg cgactatccg
     1561 cagctcgagc ggcgccgggg cggcaccgtt ctcggcagcg cctggaacgc ctttgtggct
     1621 atcggcctgt ccctggtgct gtgggtggtc acgatcccgc tgtggctgct gggtccgatc
     1681 gcggcggtgc tgccgctgtt gctgtctgcg tacctgaacc agcggctgtt ccgctacgac
     1741 gcgctgtcgg aacacgccag tcgcgaggag atgcagcgca tcttcgcgga ggcaagagga
     1801 aagctgttcc tcctcggcct gatagccggc gtgctctact tcattccgcc gttcaatctg
     1861 atcggcccgg tgttcgcggc gctggcgttc gtgcatttgt gcctgacgga gcttgagagc
     1921 ctgcgctcca cggaatcgga aatccgtgga gaacggctct tacgctaagg ggcgagggac
     1981 gaggggcgag ggacgaggga tgaggaagca gaaacatagg tcaggcgctc ttcttgttaa
     2041 gcttcttcgt cagacttttc tgcaggcctc tgatcattcg tccaatctcg tgcgaaacaa
     2101 tcagcgcgtg ttcgacatct tctttggaaa ggagctggag tttctggcaa agcagcaact
     2161 gagtttcaag ttcggccatg gagccacgag acacgagcag atatcgtagg tactctttgg
     2221 tcgattccct cgcgtgcccc tcagcgatat tcgaaggaat ggatacttgc agccctttgg
     2281 agttgcgaac taagcccgta tatttcttcg cgaggcaatc tggcggtgag tttatagacg
     2341 agagccgcaa gttccattgc gcgttgccag accgccagat ccctatagct cgcgatcacg
     2401 acgttccctc atccctcgcc cctcgttcct cgtccctgcc gtgatttaca tcctttcgta
     2461 aatcgcggct attccttgtc ctcccccgat gcacatggtc accagcgcgt acttgccgcc
     2521 gatgcgctgc aactcgtaga gcgccttcac cgaaatcagg cagccggtgg cgcccacagg
     2581 atggccgagc gcgaccgcgc cgccgttcgg gttggtcttc ttggggtcga aaccgagatc
     2641 cttcgccacc gcgcaggcct gcgccgcgaa ggcctcgttg gattcgatca cgtccaggtc
     2701 ggcgaccttc aatcccgctt tcttcatcgc gttttgcacc gcagggatcg ggcccaggcc
     2761 catcaaactc ggttccacgc cggcatggcc gtaggccacc agccgcgcca tcggcttcag
     2821 ccctcttttt tcggcaagct tgcgctccat cagcaccact gccgcggcgc cgtcgttgat
     2881 tcccgaagcg ttgcccgggg tgacggaacc gtctttcttg aacacaggct tgagtttgcc
     2941 gaggctgtcc atggtggtct cgacgcgcac gtgctcgtcc gtgtcgaaat tcttcgtgcc
     3001 ctttcggtcc ttgatctcga tcggcaggat ctgcgacttg aaccacccgt tcttgatcgc
     3061 attggccgcg cgacggtggc tttccaccga gaacccgtcc tgcgtttcgc ggctgatgcc
     3121 gtatttcgcc gcgacgtttt ccgcggtcac gcccatgtgg ctggtgtcga acggatcgga
     3181 aagcgcgccg atcagcgaat ccatcaccac ctgatcgccc attttcgagc cccaacgggc
     3241 gctcggcagc cagtaattgg cgcggctcat cgattccacg ccgccggcga ccgcgacgtc
     3301 cgcgtcgccc agtgccacga tctgcgctgc cgtgacgata gcctgcaggc cgctgccgca
     3361 caggcgattc agcgtcaccg cgggcacttc ctttggaatg ccgccgttga tcgcgcacac
     3421 gcgcgccaga tacatgtctt tcgcttcggt gtggatcacg ttgccgaaca ccacatggcc
     3481 gacttcgtcc ggcgccacct ttgaccgcga tagcgcctcg cgcacgcagc gtgcccccag
     3541 ctcggtcggc gcgaagtcct tgagcgagcc gccgaaatcg ccgatcgcgg tgcgtacgcc
     3601 ggataccacc actacttcac gtccgttgtt cattttttct ctccatcaaa ttcgtataac
     3661 tacagagagc acagagtaca cagagaaaaa acagaaacga gaaaaagcaa ctcggggata
     3721 agagcgggat tactgttcgc tagccgttgc tgccaatttt ccccgcaatg gaccgtgaac
     3781 caacggaagg ttatcacgcg aatcctgttt tctcgctctt gcctttcctc tgtgttctct
     3841 gtgtcctctg tggtcaatag ctttatttat cgttcgatgg cgagagccac acccatgccg
     3901 ccaccgatgc acagcgaggc gaggcctttt ttcgcgtcgc gcttgatcat ttcatggatc
     3961 agcgtcacca ggatgcggca gccggaagcg ccgatcggat ggccgatggc gatggcgccg
     4021 ccgttgacgt tcactttcgc ggtgtcccag cccatgtcgc ggttcaccgc gatcgcctgc
     4081 gcggcgaagg cttcgttgat ttccagcagg tccaggtcct tgggcgtcca gccggctttc
     4141 ttcaggcaca acttcgaggc cgggatcgga ccgatgccca tgatgctcgg gtccacgccg
     4201 gcggaagagt aggccttgat gcgtgccagc ggcttgaggc cgagctcctt cgccttgcct
     4261 gccgacatca tcatgaccgc cgccgcgccg tcgttgatgc ccgaggcgtt gcccgcggtt
     4321 acggtcccgt tcttgtcgaa cgcgggcttg agtccggcca gcgattgcgc ggtggtgccg
     4381 tgacgcacga attcgtccga atcaaacacc accggatcgc ctttcctctg cgggatgatg
     4441 accggcacga tttcgtcctt gaaacggccg gacttctgtg cggcttccgc cttttgctgt
     4501 gaagcggccg cgaactcgtc ctgttcctgg cgcgtcacgc cccacttctt cgcgacattc
     4561 tcggcggtga tacccatgtg gtactggttg tacgcgtccc acaagccgtc cacgatcatg
     4621 gtgtcggtga gtttgttgtc catcatgcgc agcccatcgc gcgcaccgtt cagcacgtgc
     4681 ggggcgaggc tcatgctctc ctggccgccg gcgaccacga tctcggagtc tccggccctg
     4741 atcgcctgcg cggcaagatg cgtcgccttg agtcccgagc cgcacacctt gttgatggtc
     4801 atcgccggga ccatggccgg cacaccggcg cgaatcgacg cttggcgcgc gggattctgg
     4861 ccgacaccgg cggtcagcac ctggcccagc agcacctcgc tcaccatctc cggctttacg
     4921 ccggttttct gaaacagcgc ggtaatgacc ttggcgccga gctccgatgc gggaattctg
     4981 gagattgtgc cgccgaattt accgaccgcg gtgcgaagcg ccgcgacgat taccacgtca
     5041 tccatttgca aggactcctc gattcgggat ttgttgcaac acaagcgctg ccgggggcgt
     5101 cttgaagcgt cctgaaagcg gcggaaaaga gggcgcagtt tacgctccgg tttcggcgct
     5161 tgtcgagaag aatgttgcgg cgcggcaggc attcgttagc gcattctcgc ccggactcgg
     5221 ctctccggcg agcgtagctt ggagcggagt cgagtcatgc tcgagtggga actgccgtcc
     5281 cgcgccttac ctgctttggt gcggcgcaag agccgtgtta cgacaacgac cgggcgaggc
     5341 gataaagccg aagcgtttcc ttcgagtccc ccgggcgacc gccttcccag atcatctccc
     5401 aagccctgcc gaccttgtga acgtggtccc acccgtcctg gatgatcaac acatcgcaat
     5461 cggtacgacg gttccctttc tcgaggcgct cggtcaggat gttgccgaaa tactcta
//
LOCUS       DASQOP010000046         2153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_823055, whole genome shotgun sequence.
ACCESSION   DASQOP010000046 DASQOP010000000
VERSION     DASQOP010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823055"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="VE030_01735"
     CDS             complement(<1..689)
                     /locus_tag="VE030_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22166.1"
                     /translation="MNLKFLSKRNLVLLAAATLVIAAVLLWRLWPRAHGDFVEYPMAL
                     ATDIPTAVAIAPDGTVWFTIDSAGVMGVIRDGKIARLPTVRKIVEPIGLAVDAEGAAW
                     YGDASGAMISRMLPGGAIKSFPLGTPIARLGRLTVAADGAVWFAEGTGYGFTRLKDGV
                     LARNVPKSVRGGPYGVAVDAQGNLWGTLQSGNQIVKISPAGEIAEYEVPTRASAPSDI
                     VVDGAGNTWFL"
     gene            complement(705..1877)
                     /locus_tag="VE030_01740"
     CDS             complement(705..1877)
                     /locus_tag="VE030_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22167.1"
                     /translation="MIARSFLSMVLLAAAVCPLANAHDPSAWGGLFRSRDDGATWFPA
                     DAGLFIGGALAVAVHPQDGNHLLYGTDARLLRSRNGGRDWVTEGPDVLTGAVFAVAFA
                     PDGVGGIASTGTRIFHSADGTNWQDTLAPAGAAPARTFVLGPPVGRVYLAGAHGLFAS
                     DDGGRNWLRAGEGVLPEAPVGVLLVTVGPPETVYAVIDGKVWASGDAGQTWHMRGAAS
                     QPARVEALVADSGQAGRLWSAAADQVFVSDDAGENWKAHGRPLPEKDTSVRGLAVTEA
                     GRIIVLSTHRGVLRSVDGGQNWVQIEGTLPVHLEAGPLLRDRHDAATLYAGFSLSPYS
                     EMWRRAEQGGSLLAQIDPLSLAGGLAFLLLLIVLAIVATRWLSRRYRDGAATPRFR"
     gene            complement(1964..>2153)
                     /locus_tag="VE030_01745"
     CDS             complement(1964..>2153)
                     /locus_tag="VE030_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22168.1"
                     /translation="VQQFLAYEKCYKITGGYQNCVCAHEPLSYTDKYPLTKNSWVGMN
                     GGRLAEIALAPGEKSRKP"
BASE COUNT          419 a          746 c          644 g          344 t
ORIGIN      
        1 tcgaggaacc aggtgttgcc ggcgccgtcc acgacgatgt cgctgggggc actggcacgc
       61 gtcggcactt catattcggc gatctctccc gccggagaga tcttgacgat ctggttgccg
      121 ctttgaagcg taccccacag attgccttgc gcatccacgg caacgccgta gggcccgccg
      181 cgaaccgact tcggcacgtt gcgggctaac acgccgtcct tgagacgggt aaagccgtag
      241 ccggtgccct cggcgaacca gaccgcgccg tcggccgcga ccgtcaatcg tcccagccgc
      301 gcgatgggcg taccgagagg gaaggatttt attgcgccgc ccggcaacat gcgcgagatc
      361 atcgccccgg acgcatcccc gtaccaggcc gcgccttcgg cgtccacagc gagcccgatc
      421 ggttccacga tctttctgac cgtcggcagg cgcgcgatct tgccgtcccg gatcactccc
      481 atcacacccg cggaatcgat ggtgaaccac acggtgccgt ccggcgcaat ggccaccgcg
      541 gtcgggatgt cggtcgcaag cgccatcggg tactcgacga aatctccgtg cgcccgcggc
      601 cacaagcgcc agagcaatac tgcagcgata actaaagtgg cagctgcgag cagcaccaga
      661 ttgcgtttgg aaagaaactt cagattcatc gcgcgatttt cctttcaacg gaaacggggt
      721 gtggccgcgc cgtcacggta acgacgcgac agccagcgcg tggccacgat ggcgagaacg
      781 atcaacagca gaagaaacgc cagccccccc gcaaggctga gcggatcaat ttgcgcgagc
      841 agcgagccac cctgttcggc gcgccgccac atctcgctgt aaggactcag tgaaaagccg
      901 gcgtaaagcg tcgccgcgtc gtggcgatcg cgcagcaacg gacccgcttc caggtgcacc
      961 ggcagcgtgc cctcgatctg cacccagttc tgcccaccgt cgacgctgcg cagcacgcca
     1021 cgatgcgtgc ttagcacgat aatcctgcca gcctcggtca ccgcgagacc acgcaccgaa
     1081 gtatcttttt ccggcagcgg tcggccatga gccttccagt tctcgcccgc atcgtcactc
     1141 acgaagacct gatcagctgc cgcgctccac aggcgtccgg cctgcccgga atctgcaaca
     1201 agggcttcga cgcgcgcggg ctgggatgcg gcaccgcgca tatgccaggt ttgcccggcg
     1261 tcaccgctcg cccaaacctt gccgtcgatc accgcataga cagtttccgg cggcccaaca
     1321 gtcaccagca gaacaccaac aggcgcttca ggaagcacgc cttcgcccgc gcgcaaccag
     1381 ttacgcccgc cgtcgtcgct ggcgaacagc ccgtgggcgc cggcaaggta aacgcgcccg
     1441 accggcggcc cgagtacgaa agttcgcgct ggcgccgcgc ccgccggcgc cagcgtgtcc
     1501 tgccagttgg taccgtccgc gctatggaaa atccgggttc cggtggaggc gatcccaccc
     1561 accccatccg gagcaaacgc aaccgcgaat acggcgccgg tcagtacatc ggggccttcg
     1621 gtcacccagt cgcggccacc attcctcgat cgcaacagcc gtgcatcggt gccgtacaga
     1681 agatgattgc catcctgcgg gtgcactgcg accgcgagcg cgccaccgat aaacaagccg
     1741 gcatcggcgg gaaaccaggt cgcaccgtcg tcgcgcgagc ggaacagtcc gccccaggcg
     1801 ctcggatcgt gggcatttgc caacgggcac accgccgcgg ccagcaggac catggacaag
     1861 aagcttctgg caatcattat tgacgaaccg ggaacaagca aaaaaatgcg aggagccgat
     1921 catcggctcc tcgcatcgga gtttactacc tgcggtcagc cgattacggc ttgcgcgact
     1981 tttctccggg cgccagcgca atttccgcca gacggccgcc gttcatcccc acccaggaat
     2041 tcttggtgag cgggtatttg tcggtgtagg agagcggctc gtgggcgcac acgcagttct
     2101 gatagccgcc ggtgatcttg tagcacttct cgtaggccag gaactgctgc acc
//
LOCUS       DASQOP010000047         6291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1829382, whole genome shotgun sequence.
ACCESSION   DASQOP010000047 DASQOP010000000
VERSION     DASQOP010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6291
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1829382"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VE030_01750"
     CDS             complement(<1..308)
                     /locus_tag="VE030_01750"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HZF22169.1"
                     /translation="MKRPWSGAVLRFAFVVALLAGLGQGPASAQECGIASAHTLATRA
                     GCDILKSGGNAFDAAVAVAAVLAVVEPYASGIGGGGFFLLHRGADAFDVFIDARETAP
                     A"
     gene            complement(342..803)
                     /locus_tag="VE030_01755"
     CDS             complement(342..803)
                     /locus_tag="VE030_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22170.1"
                     /translation="MDVFYYWKKFDQDMKEGRIGWLLSDRRKMGEMRADFPDFIWAFR
                     TPQGRKGELQLVARLRWSDTPVVTVPRNDAQSEIYYDPAHADSRRFIDMDTDAHIREV
                     SSMMRREFPGAFTANFQGDNGLQPMRGDFLRKFRGIAEAYPFRPFLEEVTT"
     gene            complement(1073..4210)
                     /locus_tag="VE030_01760"
     CDS             complement(1073..4210)
                     /locus_tag="VE030_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZF22171.1"
                     /translation="MREFNLSEWALEHQPLVLFFMLALFAAGVFSYLRLGQAEDPDFT
                     FKLMVVRAVWPGASPREMEEQVTERLEKKLQETPFLDNVRSYTRAGETTIFVALKDST
                     APKEIPGVWYQVRKKIGDMRHMLPNGVIGPFFNDEFGDVFGNIYAFTGDGYSYAELKD
                     YIEEVRKEILRVPNVAKAEIIGDQEEKIYVEVSHRKLATLGIDPLSIFEVLRAQNAMT
                     PAGSIDTRSDRMYLRISGSFDSVESIREIGIQAGGRLFRLGDIAHIYRGYVDPPKEKV
                     RYMGQDAIALAVSMSKGGDILDLGRDLDAAFKRIKRELPVGIEVHQYSNQPEVVKRSV
                     DEFMKTLLEAVIIVLAVSFFSLGFRTGLVVALSIPLVLTVTFLMMKLYGIDLQRISLG
                     ALIISLGLLVDDAIISVEMMATKMEQGLDRVKAASYAYTSTAFPMLTGTLITAAAFMP
                     VGFAKSSAGEYTFSIFAVVTIALLVSWVVAVLFTPYLGYRLLPDMAHAVAPPSAFQKF
                     VLGPLHRRLPRLVPAPGAPAAHDADVYNRPFYRRFRALVNWCVAWRKTVILVTAAVFA
                     LSMFGFRFVQQQFFPSSNRPELIVDLWLPQGASFQATEVQVRRFEDRLRGDANVENYV
                     AYVGNGSPRFYLPLDQQLGHANFAQFVIVAKDNKAREIMVEKLNRILETEFSLLRGRV
                     NRLENGPPVGYPVQFRVVGNDIPAVRRIALEVAEVVRSHPNTQNVHLDWNEQVKTIRL
                     RIDQNKARVIGVSSQGLSTVLNSILTGFSITQYREKDELIEVLARAQTSERLDPGKLG
                     EINVPTQSGKWIPLTQIAEIAYDFEEGIIWRRNRQHIVTVRADTRGDVQAPVVSRQIN
                     PKLDRLRAALPQHFRIDMGGAIEESAKGVNSIVAVMPMMLIVVLTLLMIQLQSFSKTT
                     MVLLTSPLGLIGVAFILLTWNLPFGFVANLGVIALFGMIMRNSVILVDQIEQDIKAGH
                     PQWQAIVEATVRRFRPIVLTALAAVLAMIPLSRSNFWGPMAVAIMGGLISATILTLLF
                     VPALYAAWFRVRKPA"
     gene            complement(4207..5346)
                     /locus_tag="VE030_01765"
     CDS             complement(4207..5346)
                     /locus_tag="VE030_01765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZF22172.1"
                     /translation="MTRAAGTRPLHATVLRAVLLAALIAIASACDKTQESAPQPRLVN
                     VVRVSVDAADGDVAYSGEVRARYETNLSFRVAGKIVARNVEVGSSVRASAVLARLDPE
                     DQQLNTRSAQSQLEAAKSEFEQTKAELERYTDLLKKNFISQAEYERRLNAFNLAKARL
                     EQAQAQLSVSKNQAGYTELVADHAGVVTAILVEVGQVVAAGQSVMKLARTGEKEVVIS
                     IPENRLADLNATREITITLWADPSKTYRGKAREVSPSADPVTRTYAAKISILDADQGV
                     GLGMTANVYLKGIQRGTAMLLPATALFQDDGKPAVWVLDPATSQVKLVRVEVGEYVED
                     KVAILSGLKTGDILVRAGVHKLFAGEKVRIAGDGATGTQAGKAAR"
     gene            complement(5410..6132)
                     /locus_tag="VE030_01770"
     CDS             complement(5410..6132)
                     /locus_tag="VE030_01770"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HZF22173.1"
                     /translation="MSDASSSRTGLLRGVVVLAIAALGAACQHEDVRSFQGYAQGETI
                     RLAAAYSGNLSKLSAKRGAKVTEGTTLFSLADEGEVAAYDAAKKRLVELTRRRGPAKD
                     TAEIESLKAQVAQTQLKLAQKSANAPADAVIVETLYAQGDWVPAGAPVVTILPVDKIT
                     VRFAVPLAVAAHLQNGRKVTLICEHCGGTPVRATVTHVSPFAQPESQTEGLEALRYMV
                     EARPNPAQATLLIPGQPVTVVL"
BASE COUNT         1147 a         2052 c         1929 g         1163 t
ORIGIN      
        1 gccggcgccg tttcacgcgc atcgatgaac acgtcgaaag cgtcggcgcc acgatgcagc
       61 agaaagaatc cgccgccgcc gatgcccgac gcatacggct cgaccaccgc cagcacggcg
      121 gccaccgcca ccgcagcatc gaacgcattg ccgccggatt tgaggatgtc gcagcccgcc
      181 cgggtggcga gcgtgtgcgc ggaggcgatg ccgcattcct gcgccgacgc gggaccctgc
      241 ccgagccccg ccaacagtgc gacgacaaac gcaaaccgta gtaccgcacc ggaccagggc
      301 cgtttcatat cagatccgcg tctcatcccg gcgcgcccat ttcacgtagt cacctcctca
      361 agaaacggcc ggaacggata ggcctccgca atgccacgaa acttcctcag aaaatcgccg
      421 cgcatcggct gcaagccgtt gtctccctgg aagttggcgg tgaaggcgcc ggggaactcg
      481 cgccgcatca tcgaactcac ctccctgatg tgtgcgtccg tatccatgtc gatgaagcgc
      541 cgggaatccg cgtgcgcggg gtcgtagtag atttccgatt gcgcgtcgtt gcgcggaacc
      601 gttaccaccg gcgtgtcgct ccatctcagc cgggcgacca actgcaattc gcccttgcgt
      661 ccctgcggtg ttcggaatgc ccagatgaag tccgggaagt cggcgcgcat ctctcccatt
      721 ttcctgcggt cggagagcaa ccacccgatg cggccttcct tcatgtcctg gtcgaatttc
      781 ttccaataat agaacacgtc catccgttcc tcctttcgtt ctcccggtgc catgcacaga
      841 gagtaccatc ccgcccttgt ccggaaccgc aacgactggt cgcgaaacga tcgttacgat
      901 tctgccccgt ttcctgcatt ccttcgcgta cccttggcgc tgccgacacg gcgttggcat
      961 agccatccgg ggcgggaggc agcgcttcgg cgcgctaccc gcaatcggcc gactccgtcg
     1021 gtaacgtgtc cgcacgcccc tttgcgattc acgcgttatg cgccgttgag gttcaagcag
     1081 gtttcctgac gcggaaccag gcggcgtaca gcgccggcac gaacagcagc gtcaggatgg
     1141 tcgcggaaat caggccgccc atgatcgcaa ccgccatcgg cccccagaaa ttcgaacgcg
     1201 acagcgggat catcgccagc acggcggcca gcgcggtcag cacgatcggc ctgaagcgcc
     1261 gcaccgtggc ctcgacgatc gcctgccatt gcggatggcc ggccttgatg tcctgctcga
     1321 tctgatccac caggatcacc gagttgcgca tgatcatgcc gaacagcgcg atcacgccca
     1381 agttggcaac gaagccgaag ggcaggttcc aggtgagcag gatgaaggcc acgccgatca
     1441 ggccgagcgg ggaagtcagc agcaccatgg tcgtcttcga aaaactctgc aactggatca
     1501 tcagcagcgt gagcacgacg atcagcatca tcggcatcac cgcgacgatc gaatttacgc
     1561 ccttggcgct ttcctcgatg gcgccgccca tgtcgattcg aaagtgctga ggcagcgcgg
     1621 cccgcagccg gtccagcttg ggattgatct gcctcgaaac caccggagcc tgcacgtcgc
     1681 cgcgggtgtc ggcacgcacg gtgacgatat gctggcggtt gcgccgccag atgatgccct
     1741 cttcgaagtc gtaggcaatc tcggcgatct gcgtcagcgg gatccacttg ccggactgcg
     1801 tcggaacgtt gatttcgcca agcttcccgg gatccagccg ttccgaggtt tgcgcgcgcg
     1861 ccagcacttc gatcagttcg tccttctccc ggtactgggt gatgctgaag ccggtgagga
     1921 tggaattgag cacggtggaa aggccctgcg agctgacgcc gatcacgcgt gccttgttct
     1981 ggtcgatccg cagccggatg gtcttgacct gctcgttcca gtcgagatgc acgttctgcg
     2041 tattcggatg cgaacgcacc acttccgcca cttcgagcgc aatcctgcgc accgccggga
     2101 tgtcgttgcc aaccacgcga aactgcaccg gatagcccac cgggggcccg ttctcgagac
     2161 ggttgacgcg gccgcgcagc agcgagaatt ccgtttcgag gatgcggttg agcttctcga
     2221 ccatgatttc gcgcgccttg ttatccttgg cgacaatcac gaactgggcg aaattcgcat
     2281 ggccgagttg ctggtccagc ggcaggtaaa agcgcgggct gccgttgccg acgtaggcga
     2341 cgtagttctc gacgttggca tcgcccctga gcctgtcctc gaagcgcctg acctgcacct
     2401 cggtggcctg gaaagacgcc ccctgtggca gccacagatc cacgatcagt tccggccggt
     2461 tggaagacgg gaagaactgc tgctgcacga agcggaatcc gaacatggac aacgcgaata
     2521 ctgcggcggt gaccaggatg acggtcttgc gccaggccac gcaccagttc accagcgcgc
     2581 ggaagcggcg gtagaacggg cggttataga cgtcggcgtc gtgcgcggcc ggcgctccgg
     2641 gcgcaggcac cagccgcggc aggcggcgat gcagaggccc gagaacgaat ttctggaatg
     2701 ccgaaggcgg ggcgacggca tgcgccatgt ccgggagcag cctgtagccg agatagggcg
     2761 tgaacagcac cgccactacc cacgacacca gcagcgcgat agtcaccacg gcaaagatcg
     2821 aaaaggtgta ctcgccggca gaagatttgg cgaaaccgac cggcatgaaa gcggcggcgg
     2881 tgatcagggt gccggtcagc atcggaaatg ccgtagaggt gtaggcatag ctcgccgctt
     2941 tcacccggtc cagaccctgc tccatcttgg tggccatcat ctccaccgaa atgatggcat
     3001 cgtccaccag cagaccgagc gagatgatca acgcgcccag cgaaattcgc tgcaagtcga
     3061 tgccgtagag tttcatcatc aggaaggtga cggtcagcac cagagggatg gacagcgcca
     3121 ccaccagccc ggtgcggaat cccaggctga agaaactgac cgcgagaacg atgatcacgg
     3181 cttccaacag ggtcttcatg aactcgtcca cagagcgctt gaccacttcc ggctggttcg
     3241 aatactggtg gacttcgatt ccgaccggga gctcgcgctt gatgcgcttg aaagcggcgt
     3301 cgaggtccct gccgagatcg agaatgtcgc cgcccttgga catggagacc gccagcgcga
     3361 tggcgtcctg tcccatgtag cgcactttct ccttgggcgg atcgacgtaa ccgcgataga
     3421 tgtgggcaat gtcaccgagg cgaaacagcc gcccgccggc ctggatgccg atttcgcgga
     3481 tgctctccac ggagtcgaag ctcccggaga tgcgcagata catccggtcg gaacgggtgt
     3541 cgatcgaacc cgccggcgtc atggcattct gcgcgcgcag cacctcgaat atcgacaatg
     3601 gatcgatgcc gagcgtggcc agtttgcggt gggagacctc gacgtagatt ttctcttcct
     3661 ggtcgccgat gatctcggcc ttggcgacat tcggaacccg cagaatttcc ttgcgcacct
     3721 cctcgatgta gtccttcagc tcggcgtagg agtaaccgtc gccggtaaac gcgtatatgt
     3781 tgccgaagac gtcgccgaat tcgtcgttga agaaaggccc gatcaccccg ttcggcagca
     3841 tgtggcgcat gtcgccgatt tttttgcgca cctggtacca gacgcccggg atctccttcg
     3901 gcgccgtcga atccttgagg gcgacgaaga tggtggtctc gccggcccta gtgtacgagc
     3961 gcacgttgtc cagaaacggc gtctcctgca gcttcttttc caggcgctcg gtcacctgct
     4021 cctccatttc gcgcggcgag gctccgggcc agaccgcccg caccaccatg agcttgaagg
     4081 tgaaatccgg gtcttcggcc tggcccagcc tgagatagga aaagaccccc gcggcgaaca
     4141 gtgccagcat gaaaaagagc accagtggct ggtgctccag ggcccattcc gaaaggttga
     4201 attccctcat cgcgcagcct tcccggcctg cgttccggtt gcgccgtctc cggcaatgcg
     4261 aactttctcg ccggcaaaga gcttgtgcac gccggcgcgc accaggatgt ctcccgtctt
     4321 caggccggag aggatggcga ccttgtcttc tacatactcg ccgacctcta cccgcaccag
     4381 cttcacctgg ctggtggcgg ggtccagcac ccacaccgcc ggtttgccgt cgtcctgaaa
     4441 cagtgcagtg gccggcagca gcatcgccgt gccgcgctgg attcccttca gatagacatt
     4501 cgccgtcatg cccagaccca cgccctgatc ggcgtcgaga atggaaatct tggcagcgta
     4561 cgtgcgggta accggatcgg ctgaagggct cacctcgcga gccttgccgc gataggtttt
     4621 cgagggatcc gcccacagcg tgatggtgat ctccctcgtt gcattcagat cggcgagccg
     4681 gttctccgga atgctgatga cgacctcctt ctcgccggtg cgcgccagct tcattacgct
     4741 ctgtccggcg gccacgacct ggcccacttc caccaagatc gcggtgacga cacccgcatg
     4801 atcggcgacc agttcggtat agcccgcctg gttcttgctg acagaaagct gtgcttgcgc
     4861 ctgctcgagc ctggccttgg ccaggttgaa ggcgttcagc cggcgttcgt actcggcctg
     4921 gctgatgaaa ttcttcttca gcagatcggt atagcgttcg agttcggcct tggtctgctc
     4981 gaattccgac ttggcggctt cgagctggga ctgcgcactc cgggtattga gctgctggtc
     5041 ctccgggtcg aggcgggcga gcacggcgct ggcgcgtacc gaactgccga cttcgacatt
     5101 gcgcgccacg atcttgccgg caacgcgaaa gctcaggttg gtctcgtagc gcgcccgcac
     5161 ctcgccggag taggcgacgt ctccgtcagc cgcatccaca ctgacgcgca cgacattgac
     5221 caggcgcggc tggggcgcgc tctcctgcgt cttgtcacat gctgaggcaa tcgcgatcag
     5281 ggcagccagc aagacggcac gcagcacggt cgcgtgcagc ggccgggtgc cggctgcacg
     5341 ggtcattctc gcaccggagg aaagtggaac tcggggacca tgactgaacc gccggcttct
     5401 gccgtggcgt cagagcacca cggtgaccgg ctgccccggt atgagcagcg tagcctgcgc
     5461 cggattcggc cgtgcctcaa ccatgtagcg cagggcttcc aggccttccg tttgcgactc
     5521 cggctgcgca aacggcgaaa catgcgttac ggtagcccgg accggcgtgc cgccgcagtg
     5581 ttcgcatatg agcgtcactt tgcggccgtt ctgcaaatgc gctgccaccg caagtggcac
     5641 cgcaaagcgc accgtgatct tgtcgaccgg caggatcgtc accaccggcg cccccgccgg
     5701 gacccagtcg ccctgtgcgt acagcgtttc gacgatgact gcgtccgcgg gcgcgttcgc
     5761 cgatttttgc gccagcttca attgcgtctg cgccacctgc gcctttagac tttcgatttc
     5821 cgccgtatcc tttgccgggc cccggcggcg cgtcagctcc accagccgtt tcttcgcggc
     5881 atcgtacgcg gcgacctcgc cttcatcagc cagcgagaac agcgtagtgc cttcggtcac
     5941 cttggcgccg cgcttggccg acagcttgga cagattgcca gaataggccg cggccaggcg
     6001 gatggtttcc ccttgcgcgt atccctggaa actgcgcacg tcctcatgct ggcacgcagc
     6061 accgagcgcc gcaatcgcca gcacgacgac gccgcgtaac aacccggtac gcgaggagga
     6121 tgcgtcgctc accgggcgcc ttccctgagc agggcgttga tgtcggcgag gtcggcttcg
     6181 gcgagcttgc ccaccgcggc cttgagccgc agctggctca ggatggtgtt gtagagcgcc
     6241 tgcgcgaggt cgcgccgcgc ggcactcaat tgctgctcgg cgttgagcac g
//
LOCUS       DASQOP010000048         4531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1427769, whole genome shotgun sequence.
ACCESSION   DASQOP010000048 DASQOP010000000
VERSION     DASQOP010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4531
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1427769"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="VE030_01775"
     CDS             <1..721
                     /locus_tag="VE030_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HZF22174.1"
                     /translation="CTIGLNSKEANNEALAEQVLEVLPRYLMKEGVVAVGEIGYDDQT
                     PVEDKYYRAQLSLAKEFDMLVQVHTPHRDKKSGTTRSMDVAIEMGIDPERLVIDHNNE
                     ETVQEVLDRGFWAAFTIYPHTKMGNERMVEIARKYGSDHIFIDSSADWGVSDPLAVPK
                     TGRLMLERGITAGDVEAICYGNALKAFGQSGQMKEEHWLDPEPIDQRMLFEGNSVLRG
                     QAPRVGEPPAVPRHGDATVFK"
     gene            755..1957
                     /locus_tag="VE030_01780"
     CDS             755..1957
                     /locus_tag="VE030_01780"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HZF22175.1"
                     /translation="MSIELVRAPAESEAVYNQEFSVRYDYPVHFTEHLFARDNPVFVQ
                     AMVRREPGKRHRFVVFVDANVAASWPTLVHDIAAYAGAHADSLELIGRPEVVSGGEQV
                     KNDPALVTTLQHKLVEMGIDRHAFVVGIGGGAFLDMIGFVAATTHRGIRHVRVPTTVL
                     GQNDSGVGVKNGINAFGVKNLLGTFAPPFAVLNDFVFLRTLHARDKIAGVAEAVKVSL
                     IRDGQFFNWLEANAEALKECAPAAMARMIRRCAELHMRQIAHGGDPFETGSARPLDYG
                     HWSAHKLEALTQHELRHGEAVAIGLALDTRYSVQIGMLAPGGEERVYKLLKRLGFYLW
                     HPAMETRDEQGRLVILRGIEEFREHLGGELTITLLRDLGRGEEVHQMDNNEILHAMTW
                     LRRRETGQ"
     gene            1954..3156
                     /gene="eboE"
                     /locus_tag="VE030_01785"
     CDS             1954..3156
                     /gene="eboE"
                     /locus_tag="VE030_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metabolite traffic protein EboE"
                     /protein_id="HZF22176.1"
                     /translation="MRLGAFGSHLTYCSNIHPGETWAEVRANIEHFFPAVRDRVAPGQ
                     PFGIGLRLSAQASRALTDPATLEEFRDFLQRNKLYVFTINGFPYGTFHGTRVKEEVYL
                     PDWRDSERLRYTDELADLLAGLLPGGVEGSVSTVPGAFKPAMTGNNGADVERIADNLL
                     RHVAHLVELERRSGKRVMLAIEPEPHCYLETVDESIGFFQRHLFSAQAVRRLGELAGL
                     ERQAASVALHDHLGLCLDLCHAAVEFEDPVTCLQRLEKAGIRVHKMQISAGLRVAELT
                     PEALKALRQFDDPVYLHQVVEDGPDGVRRFADLPAALATVNGHAAGREWRVHFHVPIF
                     LDDLGAFSSTRFFIRKVLDRHRVNPISSHLEVETYTWNVLPAHLQAGGMEQAIARELN
                     WVKAELAS"
     gene            3153..4025
                     /locus_tag="VE030_01790"
     CDS             3153..4025
                     /locus_tag="VE030_01790"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HZF22177.1"
                     /translation="MKLSVALRLGRVSNLPTVWTNVFTGVALAGAAAADARLAILLGS
                     LSLFYVGGMFLNDAFDSAFDAGSRPERPIPSGQVSAAQVYAAGFGMLVAGVGLLAWAG
                     YGFGPFTQWRPVLAGIALAIAITFYNWHHKDNPLSPLVMGVCRLLVYIAAAYAVASVL
                     PRQVFIAAIVLLCYLIGLTYSAKQEHLDRLGAVWPLAFLAVPVAYGIWLAIRQSAALL
                     PLLLFIGWTLYALWLLRRRAKGDVPRAVVSLIAGIALLDSVILAGHGAPLLAGLAVVA
                     FVLTLSLQRWVSGT"
     gene            4150..4410
                     /locus_tag="VE030_01795"
     CDS             4150..4410
                     /locus_tag="VE030_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22178.1"
                     /translation="MVDEKIKVRVTETGQVLDVVVLNKRAERIVVVLGEGVHNVKCDL
                     TPTRNGMGYAGSVMGRELIYERSRDQVQADIDRLNPALRKSR"
BASE COUNT          831 a         1422 c         1500 g          778 t
ORIGIN      
        1 ttgcaccatt ggcctgaaca gcaaggaagc caacaacgag gcgctcgccg agcaggtgct
       61 ggaagtgctg ccgcgttacc tgatgaagga aggcgtggtg gcggtgggcg agatcggcta
      121 cgacgatcag accccggtgg aagacaagta ctaccgcgcg cagttgtcgc tggcgaagga
      181 attcgacatg ctggtgcagg tgcatacccc gcaccgcgac aagaaaagcg gaaccacccg
      241 cagcatggac gtcgccatcg aaatgggaat cgaccccgag cgcttggtga tcgatcacaa
      301 caacgaagag accgtgcagg aagtgctcga tcgaggtttc tgggcggcgt tcacgatcta
      361 tccgcacacc aagatgggca acgagcgcat ggtcgagatc gcgcgcaagt acggttcgga
      421 ccacatcttc atcgattcca gcgcggactg gggcgtatcc gatccgctgg cggtgccgaa
      481 gaccgggcgc ctgatgctgg agcgcggcat taccgccggg gacgtcgagg cgatctgtta
      541 cggcaacgcg ctcaaggcat tcggccagag cggccagatg aaggaggaac actggctcga
      601 tccggaaccg atcgaccagc gcatgctgtt cgagggcaac tcggtgctgc gcggccaggc
      661 gccgcgcgtc ggggagccgc cggcggtccc gcggcacggc gacgcaaccg tattcaaata
      721 agccgggcag cacaccggca gacgggacat acagatgagc atcgagctgg tcagggcacc
      781 cgccgaatcc gaggcggtct acaaccagga attctcggtc cgctacgact acccggtgca
      841 tttcaccgag cacctgttcg cccgcgacaa tccggtgttc gtgcaggcga tggtgcgccg
      901 cgagcccggc aagcgccatc gcttcgtcgt tttcgtggat gccaacgtcg cggcaagctg
      961 gccgacgctg gtgcacgaca tcgccgcgta tgcgggagcg cacgcggatt cgctggaact
     1021 gatcggccgt cccgaagtgg tgtccggcgg cgagcaagtg aaaaacgacc ccgcgctggt
     1081 gaccacactg cagcataagc tggtggaaat gggcatcgac cgtcacgcat tcgtggtcgg
     1141 cattggcggc ggcgccttcc tggacatgat cggcttcgtc gcggctacca cgcatcgcgg
     1201 catccgccac gtgcgcgtgc ctaccaccgt gctcggccag aacgattccg gcgtcggggt
     1261 gaaaaacggc atcaacgcct tcggcgtgaa gaacctgctc ggcactttcg cgccgccgtt
     1321 cgcggtgctc aacgacttcg tcttcctgcg cacgctgcat gcgcgcgaca agattgccgg
     1381 cgtggccgaa gcggtgaagg tatcgctgat ccgcgacggc cagttcttca actggctgga
     1441 agccaacgcc gaggcgctga aagaatgcgc gccggctgcg atggcgcgca tgatccggcg
     1501 ctgcgccgag ctgcacatgc gccagatcgc gcacggcggc gatcccttcg agaccggcag
     1561 cgcgcgcccg ctcgactacg gccattggtc cgcgcacaag ctggaggcgc tcacccagca
     1621 cgaactgcgt cacggcgaag cggtcgccat cggcctggcg ctggacacgc gctattcggt
     1681 gcagatcggc atgctggcgc ccggcggcga ggagcgggtg tacaagctgc tcaagcgcct
     1741 gggcttctac ctgtggcacc cggccatgga aacccgcgac gaacagggcc gcctggtcat
     1801 cctgcgcggc atcgaggagt tccgcgaaca cctcggcggc gaactcacca tcacgctgtt
     1861 gcgcgacctc ggccgcggcg aggaagtaca ccagatggac aacaacgaga tcctgcatgc
     1921 gatgacgtgg ttgcgccggc gggagaccgg ccaatgaggc tcggggcgtt cggcagccac
     1981 ctgacctact gcagcaacat ccatccgggc gagacctggg ccgaggtacg ggccaacatc
     2041 gagcatttct ttccggccgt gcgcgaccgc gtggcgccgg gccagccgtt cggcatcggc
     2101 ctgcggctgt cggcgcaagc gtcgcgggcg ctgaccgatc cggcaacgct ggaagagttt
     2161 cgcgacttcc tccagcgcaa caaactctac gttttcacaa tcaacggctt tccctacggc
     2221 accttccacg gcacccgcgt gaaggaagag gtctatctgc ccgactggcg cgattcggag
     2281 cggctgcgct ataccgacga gctggcggac ctgctcgccg ggctgctgcc ggggggcgtc
     2341 gagggcagcg tcagcacggt gccgggcgca ttcaagccgg cgatgacggg caacaacgga
     2401 gccgacgtgg agcgcatcgc cgacaacctg ctgcgtcatg tcgcgcatct ggtcgagctt
     2461 gaacggcgta gcggcaagcg cgtcatgctg gcgatcgaac cggagcctca ctgctatctg
     2521 gagacggtgg acgagagcat cggatttttc cagcggcatc tgttcagtgc gcaggcggtg
     2581 cggcgcctgg gcgagcttgc cggcctggag cggcaggccg cgtcggtggc gctgcacgat
     2641 catctgggcc tgtgtttgga cctgtgccat gcggcggtgg agttcgagga cccggtcact
     2701 tgcctgcagc gcctggaaaa ggccgggatc cgcgttcaca agatgcagat cagtgccggc
     2761 ctgcgggtgg cggaactcac gccggaagcg ctgaaggcgc tgcgccagtt cgacgatccg
     2821 gtttacctgc accaggtggt ggaagacggc ccggacggcg tgcgccgctt tgccgatctg
     2881 cccgcagcgc tggcgacggt aaacgggcat gcagccggtc gcgaatggcg ggtgcacttc
     2941 cacgtaccca ttttcctcga cgacctcggc gcgttttcct cgacgcgctt tttcatccgc
     3001 aaggtgctcg accggcatcg cgtcaacccg atctcatcgc acctcgaagt cgagacctac
     3061 acctggaacg tgctgcccgc gcatttgcag gccggcggca tggaacaggc catcgcgcgc
     3121 gagttgaact gggtaaaagc agagttggct tcgtgaagct gtccgtcgcg ctcaggctcg
     3181 ggcgcgtttc caacctcccg accgtctgga ccaacgtatt caccggcgtg gcgctggctg
     3241 gcgccgccgc agcggatgcg cggctcgcca tcctgctggg gtcgctgtcg ctgttctacg
     3301 tcggcggcat gttcctgaac gacgccttcg acagcgcttt cgatgccggg tcacgcccgg
     3361 aacgcccgat cccatcgggg caggtgagcg cggcgcaggt ctacgctgcc ggcttcggaa
     3421 tgttggtggc tggagtgggg ctgctcgcgt gggccgggta cggtttcggg ccgttcacgc
     3481 aatggcgtcc ggtgctggcc gggattgcgc tggcgatcgc catcacgttc tacaactggc
     3541 accacaagga caatccgctc agcccgctgg tgatgggcgt gtgcaggcta ctggtctaca
     3601 ttgccgcggc gtacgcggtg gcgagcgtct tgccgcggca ggtgttcatc gcggcgatcg
     3661 tgctgctgtg ctacctgatc gggctgacct atagcgccaa gcaggagcat ctcgaccgcc
     3721 tcggagccgt ctggccgctc gcgtttctgg cggttccggt cgcctacgga atctggctcg
     3781 cgatacggca atcggcggca ttgctgccgt tgctgctctt catcggctgg acgctgtatg
     3841 cgctttggct gctgcgccgt cgcgcaaaag gcgacgtgcc gcgcgcggtg gtcagtctca
     3901 tcgccggtat agcgctgctc gattcggtga tcctcgccgg ccacggcgcg ccgctgctgg
     3961 ccgggctggc ggttgtggcg ttcgtgctga cgctgtccct gcagcgctgg gtctccggca
     4021 cttaatccaa ttcaagagcg ttgacgcaaa gttcgcaaag gaaaaaagta caggacgcaa
     4081 agaaaagcaa ttgagtgttc actgaaaagt actacacttc ttcacccttc acccgcctcc
     4141 gacaaaacca tggtcgacga aaaaatcaag gtacgcgtca ccgaaaccgg ccaagtcctc
     4201 gatgttgtcg ttctcaacaa gcgcgccgag cggatcgtgg tggtgctcgg cgaaggcgtc
     4261 cacaacgtca aatgcgacct gactccgacc cgcaacggga tgggctacgc cggcagcgtg
     4321 atggggcgcg agctaatcta cgaacgcagc cgcgatcagg tccaggccga catcgacagg
     4381 ctcaatcctg ctcttcgcaa gtcgcggtag gaaactgtcg tgacggcgta acggccgtga
     4441 cgatcgtgac gacgtaactg cgtgaaaatc gcaagagcaa ggctcatttt caataccccc
     4501 ttgggctact caaagagaaa ggagagaacg c
//
LOCUS       DASQOP010000049         6923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2637191, whole genome shotgun sequence.
ACCESSION   DASQOP010000049 DASQOP010000000
VERSION     DASQOP010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6923
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2637191"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VE030_01800"
     CDS             <1..253
                     /locus_tag="VE030_01800"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015877665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HZF22179.1"
                     /translation="HYTGWLTDGTKFDSSKDRNDPFVFGLGQGHVIRGWDEGVQGMKV
                     GGKRKLTIPPALGYGARGAGGVIPPNATLIFEVELLNVS"
     gene            691..1398
                     /locus_tag="VE030_01805"
     CDS             691..1398
                     /locus_tag="VE030_01805"
                     /inference="COORDINATES: protein motif:HMM:NF023984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3750 domain-containing protein"
                     /protein_id="HZF22180.1"
                     /translation="MPRGPAGLAPDPATTPEPVIQVYCARCLGWRRHFGVHSWIAVKP
                     ACAKNYTIYEVTYWHLRRRGSAVATRKRAPDQPWFGNVVKLLAERRGDDVGLLIERIE
                     KSIREYPFAGQYVAWPGPNSNTFVAHIARLVPELGLDLPPTAIGKDYLGRRWASTAPS
                     GNGFQFSIFGIFGMLVSGVEGFEVNILGLTFGFDPFTLALKLPLIGRLGVPRSIRWET
                     KATNALTPSHIGDEVTR"
     gene            complement(1520..1915)
                     /locus_tag="VE030_01810"
     CDS             complement(1520..1915)
                     /locus_tag="VE030_01810"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HZF22181.1"
                     /translation="MIVTPVYAGLLVLWFLVLAWRVIRRRGHGISLGDGGDAAMLRVI
                     RGHANFAEYVPLALLLMAILELCNFSAYVLHGLGAMLLIGRLLHGYALSFTDKFQFGR
                     FWGTTLTFLVLGVGAVLCVWQGIQGMTAP"
     gene            complement(1993..2451)
                     /locus_tag="VE030_01815"
     CDS             complement(1993..2451)
                     /locus_tag="VE030_01815"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZF22182.1"
                     /translation="MLRQYIHALRGFAALRAAAGGGGAQDCSGTITVEEALKAEQDRY
                     AAQMKGDVGAMQRLIGDDLVYIHSSTVQDTKASFIESIRSGNVKYRKMKRGEVKVRTY
                     GCIAIVSGGASFEVTVKAEDRTLNLLFHAVYAKRAAGIQFVSWQATKLPA"
     gene            complement(2559..3491)
                     /locus_tag="VE030_01820"
     CDS             complement(2559..3491)
                     /locus_tag="VE030_01820"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HZF22183.1"
                     /translation="MKIAVIGTGAMGSVYAGLLASAGHEVWASDTWREHIEVIHKNGL
                     RVEGASGDRTVRVHATMRPADAGVCDLVIIATKAMHVAQAAESAKPLLGPGTVVLSIQ
                     NGLGGPDTAARVLGCERVAIGVVGGFGASMKGPGHAHHNGWELVRLGEFSGPVTPRLE
                     KIAEVWRSGGFKVKCFDDIDQLVWEKLICNVCYSGTCAVTERTIIEVIEDPDAWRVAS
                     GCAAEAFSVAKARKVRLDFDDPVAYVRAFGMKIPNARPSMLLDHMAGRISEIDAINGA
                     IPPAAAAIGVAAPCNEVISGLVRAKERRMGVRGR"
     gene            4055..4318
                     /locus_tag="VE030_01825"
     CDS             4055..4318
                     /locus_tag="VE030_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22184.1"
                     /translation="MNVTKLILAAGFAVVSTGAFAETGVGTVRIDNVANVYGRAGVPN
                     VKVYGTVASQPTDVAVPGGNITGTGDTKIAVTSGKNVVAFGRS"
     gene            4471..5571
                     /locus_tag="VE030_01830"
     CDS             4471..5571
                     /locus_tag="VE030_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HZF22185.1"
                     /translation="MKITSIETIVLNIPYSSGGPSDTEAWGGTAWRTADTLLVKVGTD
                     AGITGWGEAFGYNVIPATRVAIDQILAPMCLRRDPLKIEELMLELQQKLHIFGRSGPI
                     IFGLSGLDVALWDIAGKAAGQPVHKMLGGAKSPRLTCYASMVRYTEPKVVAANVARAL
                     GLGFRHIKLHEIELAATRAAREAAGPDVDIMLDVNCPWSAQEALAMAEKLRPLNLRWL
                     EEPVWPPEDYAALAQVRARGCIAVAAGENATTLTQFQHLLEARAVDYLQPSPAKMGGI
                     SELRKVFALAAQHQVAVAPHSFYDGPAFLGGLHVNAAMGRNSLVEWRFFDLESRLYGD
                     AAIPEGGSIAVPQGPGLGFDPDPDVIRLYRAN"
     gene            5705..6496
                     /locus_tag="VE030_01835"
     CDS             5705..6496
                     /locus_tag="VE030_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HZF22186.1"
                     /translation="MAGKERNLKSVAASSVRQRVSKEEWEMRCDLAAAYQLAALFKWT
                     DLIYTHFSARVPGTEHLLINPYGLMFEEITASTLVKIDKDGSIIDDPTGLGINYAGFV
                     IHGCVHEARPEINCVIHTHTRAGVAVSAQKCGLLPLSQHSLRVYAMLSYHDYEGIALD
                     LDERTRLAGDLGPRSKTMILRNHGLLTLGDTVREAFELMYYLDCACQIQVDALAGGRV
                     DVGLISEQTAHKGFEQFGRPGGSEVHKEWPALLRLLDRKGIAYRN"
BASE COUNT         1317 a         2263 c         2063 g         1280 t
ORIGIN      
        1 tcattacact ggctggctca ccgacggaac caagttcgat tccagcaaag accgcaacga
       61 tcctttcgta ttcgggctgg gccagggcca tgtcatccgc ggctgggacg agggggtgca
      121 aggcatgaag gtgggtggca agcgcaagct cacgattcca ccggcgctcg gttacggcgc
      181 ccgcggcgcc ggtggtgtca tcccgccgaa cgcgacgctg attttcgaag tggaactgct
      241 gaacgtgagt tgaaacttgc gcgcactgta aacggcgccg ttccgcggaa cgaatattcg
      301 aacgcggcgt gccggatgga gttcggtgct cggcagttaa tgatcgcgcg acttgtgcgc
      361 tggtcgaatt cgactatttg ctacacgagc gatcgagctt tccaaatcgg ctgtaacgga
      421 tcatctacat cgaccggcgt ttgacccttt cgcctgcgtc cctgcccggc cccgggtcat
      481 ctgcacgcac gcgaaagtag gccaaaggaa ccacttttgc acgctgaccg actgcaactg
      541 acgccaactc gcccacatac cattcgaatc gtaactttga ttctccatgg atcggacatg
      601 agtgttttcc gcggcatcag aagtttctcc gtcgggccga ttgcagcttt tgttgcgcag
      661 ggggtcgcgg gtttgcgtga ttggcgcagc atgcctcgcg gaccggccgg cctcgcgccc
      721 gacccggcga cgactcccga gccggttatt caggtgtact gcgcccgttg tctgggatgg
      781 cgacgccatt tcggcgtgca ctcctggatc gcggtgaagc cggcgtgcgc gaaaaactac
      841 acgatctacg aagtgaccta ctggcacttg cgccggcgcg gctcggccgt ggcgacgcga
      901 aaacgcgcgc ccgaccagcc ctggttcgga aacgtggtca agctgctcgc cgaacgacgc
      961 ggcgacgatg tcggtttact gatcgagcgc atcgaaaagt cgatccgaga atatcccttc
     1021 gccggacaat acgtcgcctg gcccggaccc aactcgaaca cctttgtcgc tcacattgcg
     1081 cggttagtcc ccgaactcgg gctggaccta ccgccaacgg cgatcggcaa ggactatctc
     1141 ggccgccgat gggcgtcaac cgcgcccagc ggaaacggct tccagttttc gatcttcggc
     1201 atattcggca tgttggtgag cggagtcgag gggttcgagg tcaatatcct gggcctcacg
     1261 ttcggtttcg atccatttac ccttgcgctg aaactcccgc tcatcgggcg tctgggcgtc
     1321 ccccgttcaa ttagatggga gactaaggcg accaacgcgt tgacgccctc acacatcggc
     1381 gatgaagtga cgaggtgaac gaagtattga gttacttact caactcacga cgttcgcgtt
     1441 acgggcgcgc tgttgccggc cggtagcaaa tggagagtgc caaggcctga ttctcccggc
     1501 agtctgccgg tcgccgcatt cagggcgcag tcattccctg tattccctgc catacgcaca
     1561 ggacggcgcc aacgcccaga acaaggaagg tcagcgtcgt cccccagaag cggccgaact
     1621 ggaatttatc cgtgaacgag agcgcatagc cgtgcaacag ccggccaatc aacagcatcg
     1681 cacccagtcc atgcaggaca taggccgaaa agttgcacag ctcaaggatc gccatcagca
     1741 gcagcgccag cggcacgtac tcggcgaaat tggcgtgccc gcggatcacc cgaagcatgg
     1801 ccgcatcgcc gccgtctcca agtgaaatgc cgtgaccgcg gcggcgaata acgcgccagg
     1861 ccaggaccag gaaccagaga accagcaacc cggcatacac aggcgtgacg atcacggcgc
     1921 actccttcgt ctgggaaccg agtcgacgtt gctaccgttc cggctgaacc ggttgacgat
     1981 tcccgcggtc cgttacgccg gcaatttggt cgcctgccag gacacgaact ggatacccgc
     2041 cgcgcgcttc gcgtaaacgg cgtggaacag caggtttagg gtccggtcct ctgccttcac
     2101 ggtcacctcg aagctggccc cgccgctgac gatcgcgatg cagccgtatg tccggacctt
     2161 cacctcgccg cgcttcatct tgcggtactt cacgttcccg gaccggatcg actcgatgaa
     2221 gctcgccttg gtgtcctgca cggtgctcga gtggatatag accaggtcat caccgatcaa
     2281 tcgttgcatc gcccctacgt cgcctttcat ctgcgcagcg tagcgatcct gctccgcctt
     2341 cagcgcttcc tccacggtga tcgtgccgct gcagtcctgc gccccccctc cgccggccgc
     2401 ggctcgaagc gcagcgaatc cgcgaagtgc gtgaatgtac tgtctcagca tttttttctc
     2461 ctccgtttga aattcattcc tagtgtcctc ttccgccgat tcgtgggaca acttgcgaac
     2521 cttggaaggc agacctcata tccataaaga cccgcatctc agcggccgcg cactcccatg
     2581 cgccgctctt tggcgcgcac caggccactg atcacttcgt tgcagggagc ggcgacgccg
     2641 attgcggcgg cggccggcgg aatggcgccg ttgatggcgt cgatctccga gatccgcccg
     2701 gccatgtggt cgagcagcat ggaagggcgg gcattgggga tcttcatgcc gaatgcgcgc
     2761 acgtaggcca ccgggtcgtc gaagtccagg cggaccttgc gcgccttcgc cacggagaaa
     2821 gcttcggccg cgcagcccga ggccacacgc caggcgtcgg gatcctcgat gacctcgatg
     2881 atggtgcgct cggtgaccgc gcaagtcccc gagtagcaga cgttgcagat gagcttttcc
     2941 cacaccagtt gatcgatgtc gtcgaagcac ttcactttga agccgcccga gcgccacacc
     3001 tcggcaattt tctccaggcg cggcgtaacg ggcccggaga attcgcccag ccgaaccagt
     3061 tcccagccgt tgtgatgggc gtggccgggt cctttcatcg acgcaccgaa accgccgacg
     3121 acgccgattg ccacgcgctc gcagccaagc acgcgcgccg cggtgtccgg gccgcccaga
     3181 ccattctgga tcgacagcac caccgtaccg gggccgagca gcggcttcgc cgattcggcg
     3241 gcctgcgcga catgcatcgc cttggtggcg atgatcacaa gatcgcaaac acccgcgtcc
     3301 gcgggccgca tcgtcgcatg cacccgcacc gtccggtccc cggacgcgcc ttcgacgcgc
     3361 agaccgttct tgtggatcac ctcgatgtgc tcgcgccagg tatcacttgc ccacacttcg
     3421 tggcctgccg aggcgagcag tccggcgtat accgacccca tcgcaccggt gccgatgacg
     3481 gcgattttca tctgatcgat ggctgttcgc gcgaatgaac gacgaacctg aaagaaaccg
     3541 cattcggaaa aaaaccatta actgccccac gcattttttc cccttgtcct cgagtacctc
     3601 ggcttgaatc gcggcactcg tcgtgaggcc gctcgccgcc ctgatcgcgt agcgaccaac
     3661 gccgatcatt tgcgtcagac cgtcattcgc cgccgaaccc gattttgagg gcccgcagac
     3721 gcgcaggcaa ggacatcggc ctgcatccca aaacaccgcc ggccacgacc ggcaaggttc
     3781 tgaacgtcaa gcgccttttt gcctcgggcc aactcccgcc gtccgtgagg acctctccgt
     3841 tcggcgccgc ccaggcatcg atgactgtct cgcataaaac ttacattcca gaatggaata
     3901 aatattatgg cgactcgtag cctattgtgc gacacagcat tgctgtttaa tgcacaccat
     3961 gcgtggggcg aaagggtttt cgtctcaacc tgggcgaatc caggtgcagt cgcacaacat
     4021 atggattgat tccacaaaca ataggagtga caccatgaac gtaaccaaac tgattctcgc
     4081 cgccggtttt gccgtcgtca gcaccggtgc atttgctgaa actggcgtgg gcaccgtccg
     4141 gatcgacaac gtcgccaatg tatacggccg tgccggagtc ccgaacgtga aggtctacgg
     4201 taccgtggca tcccaaccga ccgacgtcgc cgttcccggc ggcaacatca ccggcacggg
     4261 tgacaccaag atcgccgtga ccagcggcaa gaacgtcgtc gcattcggcc gttcctaagc
     4321 cgcccgcagc aaaccgaacg gcgcactgct ccaggcagtg cgccgttttt atttccgagc
     4381 cgccttcctc cgtccgcagt aggcaggccc ccagagtctc gcctatcatt tcgcctcgcc
     4441 tttcccgcca ttacgcagca cacgagagaa atgaaaataa catccatcga aaccatcgtg
     4501 ctgaacattc cgtacagcag cggcgggcca tccgacaccg aagcgtgggg cggcacggcc
     4561 tggcggaccg cggatacgct gctggtgaaa gtcggcaccg acgccggcat taccggctgg
     4621 ggcgaggcct tcggttacaa cgtcatcccc gccaccaggg tcgccatcga tcagattctg
     4681 gcgccgatgt gcctgcgccg cgacccgctg aagatcgaag agctgatgct ggagctgcag
     4741 cagaagctgc acatcttcgg ccgcagcggc ccgatcatct tcggcctgtc ggggctggac
     4801 gtcgcgctat gggacatcgc gggcaaggca gccgggcagc cggtgcacaa aatgctgggc
     4861 ggcgcaaagt caccacggct tacctgctac gccagcatgg tccgctatac cgaaccgaag
     4921 gtggtagcgg ccaacgtcgc tcgcgccctg gggctgggct ttcgccacat caagctgcac
     4981 gagatcgaac tcgcggccac ccgggccgcg cgcgaggctg ccggccccga cgtggacatc
     5041 atgctcgacg tgaactgccc gtggtccgcg caagaagcgc tggcgatggc ggagaaactg
     5101 cggccgctca acctgcgctg gctggaagag ccggtgtggc cgccggaaga ctacgccgcg
     5161 ttggcgcagg tgcgcgccag gggctgcatc gcggtggccg caggcgaaaa cgccaccacg
     5221 ctcacgcagt tccagcatct tctggaggcg cgcgcggtcg attacctgca gccgagccct
     5281 gccaagatgg gtggaatttc cgaactgcgc aaggtattcg cactcgccgc gcagcaccag
     5341 gtggcagtag cgccgcacag cttctacgac ggccccgcat ttctcggcgg cctgcacgtc
     5401 aatgccgcaa tgggccggaa ctcgctggtg gagtggcgct tcttcgactt ggagtcgcgg
     5461 ctgtacggcg atgcggcaat accggaaggc ggctcgatcg cggtgccgca gggccccgga
     5521 ctcggcttcg atcccgatcc ggatgtcatc cgcctctatc gtgcaaatta gtgagacagt
     5581 gagcggtaac tggtaaccag ccaggtcaac agcagtcacg gtcgccacga cgtgcgcggc
     5641 cattaccttg ctgcggcggg aggcgtatca ttttaagttc cagtggcagg ggaggatcga
     5701 aaaagtggcc ggaaaagaac gaaacctcaa gtccgtcgca gcctcgtcgg tgcgccagcg
     5761 cgtcagcaag gaagagtggg aaatgcgctg cgatctcgcc gccgcatacc agctcgcggc
     5821 gctgttcaag tggaccgacc tgatctacac ccacttctcg gcgcgggtgc ccggcaccga
     5881 gcacctgctg atcaatccct acgggctgat gttcgaggag atcaccgcct caaccctggt
     5941 gaagatcgac aaggacggca gcatcatcga cgatcccacc gggctgggca tcaactacgc
     6001 cggcttcgtg atccacggct gcgtgcacga ggcgcggccg gagatcaact gcgtgatcca
     6061 cacccacacc cgcgccggcg tcgcggtgtc ggcgcagaaa tgcggcctgc tgccgctgag
     6121 ccagcattcg ctgcgggttt acgcgatgct ctcctatcat gactacgagg gcattgcgct
     6181 cgacctggac gagcgcacgc gtctcgccgg cgacctgggc ccgcgctcca agaccatgat
     6241 cctgcgcaac cacggcctgc tcacgctggg cgacacggtc agggaagcct tcgaactgat
     6301 gtactacctc gactgcgcct gccagatcca ggtcgatgca ctcgccggcg gccgcgtcga
     6361 cgtcgggctc atctccgagc agacggcgca caagggcttc gagcaattcg ggcggcccgg
     6421 cggctcggag gtgcacaagg aatggccggc gctgttgcgc ctgctcgacc gcaaaggaat
     6481 tgcctaccgc aactgaaggt ttccacgcac gtgccctcgc tgcgccaggc cgccctctgt
     6541 gaaacgccgg ctcggtgaat tttcacccgc gttgctggcg ccgacttgct tgatccacca
     6601 tcccgcgagc gaaaacttcg gcgttcgttg acggatttcg gcagcgcccg ctgctgaaag
     6661 ctcgtcagtg gctctgtccg tttttcgact cgctcttttt ttattcacaa gcagagcaca
     6721 tttagtgtcg ggccgaaccg tcgtcttttc gacgcccccc actggcacgg ctgttgcgcc
     6781 atagcgtgtg cgcccgggga attcggtgag caagggaggt cgggtgtttt tccaagacgc
     6841 ttgaacttgc gcatcccccg ggcgcacctg ccttcgcagc gacctggtga ggcgatgcga
     6901 cgatttctgc cggcgcagtt ata
//
LOCUS       DASQOP010000050         3973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_824177, whole genome shotgun sequence.
ACCESSION   DASQOP010000050 DASQOP010000000
VERSION     DASQOP010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824177"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..169)
                     /locus_tag="VE030_01840"
     CDS             complement(<1..169)
                     /locus_tag="VE030_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HZF22187.1"
                     /translation="MSIVQNKKAFHDYFIEERFEAGLALEGWEVKAIRAGGVQLKEAY
                     VIVKNAEMFLIG"
     gene            261..698
                     /locus_tag="VE030_01845"
     CDS             261..698
                     /locus_tag="VE030_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HZF22188.1"
                     /translation="MALVEKSVLVEYSAPQMHALVEDVARYPEFLPWCGGTEVISREG
                     EITRAAIRIDYRGIRQRFSTENRSQPPQLIEMKLVDGPFRQLDGSWRFKPLGEQACKI
                     EFRLHYEFSSRLLEKLVGPVFHYIANTFVEAFVKRAQQLYGRR"
     gene            695..991
                     /locus_tag="VE030_01850"
     CDS             695..991
                     /locus_tag="VE030_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HZF22189.1"
                     /translation="MKVVVQVVYALPGAQEIVQVELEQGAPVEDAIRASGILAHHPEI
                     DLKTQRIGVWGRPVTLATGVRDRDRVEIYRALSADPKQARRRRAAAESSRNRRR"
     gene            complement(1028..1522)
                     /locus_tag="VE030_01855"
     CDS             complement(1028..1522)
                     /locus_tag="VE030_01855"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HZF22190.1"
                     /translation="MPRFRMTAGLVTTLACGCLLALPALGELYKWTDAQGKVHYSDQP
                     PPPEVKQPVAVKPRSRAAATAAPDAAGTPKAAPKSVVEQDAEFNRRRVEAAEREAAEK
                     KAAKEAAEKKKNCEQAKAQLARVQSGARITRYNEKGETVYLNDAEIEQEAARARQVAD
                     SWCK"
     gene            complement(1537..1920)
                     /locus_tag="VE030_01860"
     CDS             complement(1537..1920)
                     /locus_tag="VE030_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22191.1"
                     /translation="MFDGDEVYCDDMAGCKQMGWYQRLMYMTACIVAVFGIAAPAWAS
                     GVAAGAAQTLDAEVARTAIAQAERAVSRAAAQRMLWTTADEALRSARRAFEDGDLAAA
                     LAQARVAKEHSELAIAQKRYPPFRI"
     gene            2024..3484
                     /gene="guaB"
                     /locus_tag="VE030_01865"
     CDS             2024..3484
                     /gene="guaB"
                     /locus_tag="VE030_01865"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HZF22192.1"
                     /translation="MRVLHKALTFDDVMLVPAHSTVLPRDVSLTTRLTKNISLNIPLV
                     SAAMDTVTEARLAIAIAQEGGIGMLHKNLPPKAQAAEVAKVKRFESGVVKDPITISQD
                     MTVRDVLNLIRQHKISGLPVVDASRVVGIVTNRDLRFETNLDQPVKNIMTPRQRLITV
                     KEGVSSEDAKRLLHQHRLERVLVVNENFELRGLITVKDILKSSEHPNASKDQLGRLRV
                     GAAVGVGEGTEERVELLIEAGVDVLVVDTAHGHSQGVLDRVRWIKRKFPGAQVIGGNI
                     ATAAAAKALADQGADGVKVGIGPGSICTTRIVAGVGVPQITAINNVATGLKGTDIPMI
                     ADGGVRYSGDIAKAISAGADSVMLGGLFAGTEEAPGEIELFQGRSYKSYRGMGSLGAM
                     QQGSSDRYFQDDEDSADKLVPEGVEGRVPYKGSVVQVMQQLMGGLRSAMGYLGCATIH
                     EVRDRAEFVEITSAGVRESHVHDVQIVKEAPNYHIE"
     gene            3589..>3973
                     /locus_tag="VE030_01870"
     CDS             3589..>3973
                     /locus_tag="VE030_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914102.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HZF22193.1"
                     /translation="MQHDKILILDFGAQYAQLIARRVREAGVYCELHPFDVSEQFIRD
                     FSPRGIILSGGPSSVTEEETPRAPQVVFELGVPVLGICYGMQAMAAQLGGAVENGMVR
                     EFGYAQVRARGHSRLFDGIQDRVNGE"
BASE COUNT          766 a         1229 c         1230 g          748 t
ORIGIN      
        1 aaccgatcag aaacatctcg gcgtttttca ctatcacata cgcctctttg agctgcacgc
       61 cacccgcgcg gattgccttg acctcccagc cctcgagcgc cagaccggcc tcgaaccgct
      121 cttcgatgaa ataatcatgg aaggccttct tgttctgaac gatgctcatt tccgggtggg
      181 aatttgaaga cgcgggtgtt ttttttgcgg cgatcgtgta ttttaccagc ccctctcgcg
      241 cactctccct gacaatttca atggcgctag tggaaaaatc cgtgctggtc gagtattccg
      301 ccccgcagat gcatgcgctc gtggaggatg tcgcccgcta tccagaattc cttccctggt
      361 gcggcggcac cgaagttatc agccgcgaag gcgaaatcac acgcgctgca atcaggatcg
      421 attatcgtgg catcaggcag cgcttttcca cggagaaccg gtcgcagccg ccgcaactga
      481 tcgaaatgaa actggtggac ggtccgttcc gccaactcga cggcagttgg cgtttcaagc
      541 cgctgggcga gcaagcctgc aagatcgagt tccggctcca ctacgagttc tccagcaggt
      601 tgctggagaa actggtcggg cccgtgtttc attacatcgc caacaccttc gtcgaggcct
      661 ttgtcaaacg cgcgcagcaa ctctacggca ggcgatgaaa gtcgtcgtcc aggtggttta
      721 cgctctgcca ggggcgcagg aaatcgtgca agtcgagctg gaacagggtg cgccggtgga
      781 agacgcaatt cgtgcctccg ggatcctggc gcaccacccg gaaatcgatt tgaaaacgca
      841 gcggatcggt gtgtggggtc ggccggtcac gctggctacc ggtgtgcgcg accgcgaccg
      901 ggtcgagatt taccgggcac tgagcgctga tccgaagcag gcaagacgcc gtcgcgctgc
      961 cgcggaaagc agccggaacc ggcgccgtta aggttcttgc gacaccgggc cgttcaatgt
     1021 tgcgggccta tttgcaccag gaatccgcca cctgtcttgc acgcgcagct tcctgctcga
     1081 tctcagcgtc gttcaggtac acggtctcgc ctttttcgtt gtaacgggtg atacgcgcgc
     1141 cgctttgcac gcgcgccagc tgggccttgg cctgttcgca attcttcttc ttttctgctg
     1201 cctccttggc cgctttcttc tccgcggctt cgcgttccgc ggcttccacg cggcgccggt
     1261 tgaattccgc atcctgctct acaacacttt tcggcgccgc cttaggcgtt cccgccgcgt
     1321 ccggagccgc cgttgctgcc gcccggctgc ggggtttgac cgcaaccggc tgtttgacct
     1381 ccggcggcgg gggctggtcc gagtaatgca ccttgccctg cgcatcggtc cacttgtaca
     1441 actcgccgag cgccggcagc gccagcaggc agccgcaggc aagcgtcgtc accagaccgg
     1501 cggtcatacg aaatcttggc atgaattctc cttcatctag atgcgaaacg gtgggtagcg
     1561 cttctgcgcg attgcgagct ccgagtgctc ctttgcgacg cgagcctgcg cgagcgcggc
     1621 cgcaaggtct ccgtcctcga aagcgcgccg cgcgctgcgc aacgcctcgt ccgcggtggt
     1681 ccagagcatg cgctgggctg cggcacggct gactgcccgt tcggcctgcg cgatcgcggt
     1741 cctggcgact tccgcgtcga gtgtctgcgc ggctccggct gcgacacccg atgcccaggc
     1801 cggtgccgca attccgaata ccgcaacaat gcaggccgtc atgtacatca aacgctgata
     1861 ccaccccatc tgtttacaac ccgccatgtc gtcacaatag acttcgtcac cgtcaaacat
     1921 atccgctcac gcccttcggg tcaacgaaaa acccccaaag agttcggcat ttaccatgca
     1981 aaagtcgatc ggtataatgg cgtctttgtg aaggatcaaa agcatgcgag tcctgcataa
     2041 ggccctcacc tttgacgacg taatgctggt tcccgcccac tccaccgttc tcccccgcga
     2101 cgtcagtctc acgacgcgcc tgaccaaaaa catctcgctg aacattcccc tggtttccgc
     2161 ggccatggat acggtcacgg aagcaaggct cgccatcgcc atcgcacagg aaggcgggat
     2221 cggcatgctg cacaagaacc tgccgccaaa ggcgcaggcc gccgaagtgg cgaaggtgaa
     2281 gcgcttcgag agcggcgtgg tgaaagaccc gatcacgatc tcgcaggaca tgacggtgcg
     2341 cgacgttctg aatctgatcc gccagcacaa gatctccgga ctaccggtcg tggacgcctc
     2401 cagagtggtc ggcatcgtca ccaaccgcga cctgcgtttt gagaccaatc tcgatcagcc
     2461 ggtgaagaac atcatgactc cgcgccagcg gctgataacg gtcaaggaag gcgtgagcag
     2521 cgaggacgct aagcgtctgt tgcaccagca tcgcctggaa agggtgctgg tcgtgaacga
     2581 aaacttcgaa ttgcgcgggc tgatcactgt gaaggacatc ctcaagtcct ccgagcatcc
     2641 gaacgccagc aaggatcagc tcggacgcct gcgcgtcggg gctgcggtcg gcgtgggcga
     2701 gggcaccgaa gagcgcgtgg aactgctgat cgaagccggc gtggacgtgc tggtggtgga
     2761 taccgcgcac ggccactcgc agggcgtgct ggaccgggta cgctggatca agcgcaagtt
     2821 tcccggcgcg caggtcatcg gcggcaatat cgcaacggcc gccgcggcaa aggcgctggc
     2881 ggaccagggc gcggatggag tgaaggtcgg catcggcccg gggtcgatct gcaccacgcg
     2941 catcgtcgcc ggggtgggcg tgcctcagat caccgcgatc aacaacgtcg ccaccgggct
     3001 gaaaggcacc gacataccga tgatcgccga cggcggcgtc cgttattcgg gcgacatcgc
     3061 caaggcgatc tccgcgggcg ccgattcggt gatgctcggc ggactgttcg ccggcaccga
     3121 ggaagcgccg ggcgagatcg agctgttcca ggggcgctcg tacaagtctt accgcggcat
     3181 gggatcgctg ggcgcgatgc aacagggttc cagcgaccgc tatttccagg acgatgaaga
     3241 cagcgcggac aagctggtgc ccgagggtgt ggaaggccgt gttccctaca agggcagcgt
     3301 cgtacaggtc atgcaacagc tgatgggagg gctgcgttcg gcgatgggtt acctcggttg
     3361 cgcgaccatc cacgaagtca gggatcgcgc cgagttcgtg gagatcacca gcgccggcgt
     3421 gcgcgaatcg cacgtccacg acgtgcagat cgtcaaggaa gcacccaact accacatcga
     3481 ataggattga ggaatgagga atgaggaatg aggaatgagg aatgaggatt gagctgaaaa
     3541 cagaaccggg gttccctcag tccccagtcc tcgatcctca atcctgaaat gcagcacgac
     3601 aagatcctca tcctcgattt cggcgcgcag tacgcgcagc tgatcgcacg ccgcgtacgc
     3661 gaggcgggcg tgtactgcga gctgcatccc ttcgacgtca gcgagcagtt catccgcgat
     3721 ttcagtccgc gcggcatcat tctttcgggg gggccgagtt cggttaccga agaagaaacg
     3781 ccgcgtgccc cgcaggtggt tttcgagttg ggcgtaccgg tgctgggcat ctgctatggc
     3841 atgcaggcaa tggcggcaca actcggaggc gcggtggaaa acggcatggt gcgcgaattc
     3901 ggctacgcgc aggtgcgtgc ccgcggccac tcgcgcctgt tcgacggcat ccaggaccgg
     3961 gtcaacggcg aag
//
LOCUS       DASQOP010000051         8913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2032146, whole genome shotgun sequence.
ACCESSION   DASQOP010000051 DASQOP010000000
VERSION     DASQOP010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8913
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2032146"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1687
                     /gene="tkt"
                     /locus_tag="VE030_01875"
     CDS             <1..1687
                     /gene="tkt"
                     /locus_tag="VE030_01875"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HZF22194.1"
                     /translation="PEYGMAPGVETTTGPLGQGLANAVGMAIAEKLLAAEFNRPGHGI
                     VDHRTYVFVGDGCLMEGISHEACSLAGTLGLGKLTAVYDDNGISIDGHVSGWFRDDTP
                     KRFEAYGWHVLRNVDGHDVAAVDAAIIQAGRVADKPSLICCKTVIAKGSPNKAGSHEA
                     HGAALGDQEVAATRENIGWKYPPFEIPQDIYAGWDARAKGAELEAGWNRKFAAYAKAF
                     PQEAGEFTRRVNGELPKQWHTHTQDLLAKVNAKAESIATRKASQNAIENLAPVLPELV
                     GGSADLTGSNLTLWSGSKAVSSDSGGNYIYYGVREFGMSAIMNGMTLHGGLIAYGGTF
                     LTFSDYSRNALRMAALMKIRSLFVFTHDSIGLGEDGPTHQSVEHSASLRLIPNMNVWR
                     PCDSVESAVAWIAAIERRDGPTSLLFSRQNLPFQARSAEQIALIGRGAYVLADVDRPR
                     AVIIATGSEVALAIGAKKELEGQDIAVRVVSMPCTSVFDRQDAAYKNAVLPKGVKRVA
                     VEAGVTDYWRKYVGLDGVVVGLDRFGESAPAGDLFKFFGFTVESVVKAVKGVL"
     gene            1733..2692
                     /locus_tag="VE030_01880"
     CDS             1733..2692
                     /locus_tag="VE030_01880"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HZF22195.1"
                     /translation="MGKAVRLVGDIGGTNARFALVADGSSQPQQEQVMRCAEFEGLEQ
                     AIRRYLADHGNPGIREAALDVATGITGDFVQLTNGPWGFSIETTRQVLALDRLHVIND
                     FTALALSVPTLQPHELRQVGGGRPVAGTAIAVIGPGTGLGVSGLLPMGEGWMAVQGEG
                     GHTAFSPTTEREIEVLRWLMPQFEHVSTERVVSGMGLENLYQALCALAHATAQPLTPA
                     QITEAALAGKEVHCREALDMFCAILGAAAANLVVTLGARAGCYIGGGIVPRLGEYFAR
                     SPFRARFERKGRFSAYVAAVPTYVILAETPALRGLASLFTDRR"
     gene            2706..2993
                     /locus_tag="VE030_01885"
     CDS             2706..2993
                     /locus_tag="VE030_01885"
                     /inference="COORDINATES: protein motif:HMM:NF037968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HZF22196.1"
                     /translation="MTDVIGLVAGLLTTTAFIPQVLKIYNTKSGKDISSRMISMFSIG
                     LVLWLIYGIIIRSTPIIVTNAFTLILALAIIVLKIRYSRLHSRSRHESGRP"
     gene            3100..4113
                     /gene="gap"
                     /locus_tag="VE030_01890"
     CDS             3100..4113
                     /gene="gap"
                     /locus_tag="VE030_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HZF22197.1"
                     /translation="MTIRVAINGYGRIGRNILRAHYEGGRKHDIRIVAINDLGDAKIN
                     AHLTKYDTAHGKFPGSVSVDGEFLVVNGDKIKVCAQRNPAELPWKDLGADVVLECTGL
                     FTSKAKSGAHLSAGAKKVIISAPGDKDVDATVVYGVNHHTLKAAHTVISNASCTTNCL
                     APMVKALNDRIGVVNGLMTTIHAYTNDQVLTDVYHTDLRRARSATMSMIPTKTGAAAA
                     VGLVLLELNGKLDGFAIRVPTINVSLVDLAFVAKRATTVEEVNNAVKQASESDLKGIL
                     EYNKDPLVSVDFNHNPASSVFDSTLTKVSEGTLVKVCSWYDNEWGFSNRMLDVTVALA
                     NAK"
     gene            4226..5413
                     /locus_tag="VE030_01895"
     CDS             4226..5413
                     /locus_tag="VE030_01895"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HZF22198.1"
                     /translation="MTILRMTDLDLRGKRVFIRADLNVPQDAAGNITDDTRIRASVPA
                     IRHALNSGAAVMVTSHLGRPAEGELKPEDSLAPVAKRLSELLGVPVPLKTNWVEGVDV
                     KPGQVVLLENCRVNKGEKKNDETLARKMAALCDIYVNDAFGTAHRAEASTHGIARFAP
                     VACAGPLMAAELEALGKALTVPKRPLVAIVAGSKVSTKLSILGELAKKVDQLIVGGGI
                     ANTFMLAEGLPVGKSLIEANLVGEAKRIIEAMRAKGGAVPIPEDVVCAKEFSAHAKAM
                     VKDAGDVAADDLILDIGPKTAGRLADALGKAGTIVWNGPVGVFEFDQFSEGTKTIAHA
                     IAASPAFSIAGGGDTVAAIAKYGIEKKIGYISTGGGAFLEFLEGKKLPAVDILEQRAI
                     ARI"
     gene            5555..6988
                     /gene="pyk"
                     /locus_tag="VE030_01900"
     CDS             5555..6988
                     /gene="pyk"
                     /locus_tag="VE030_01900"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HZF22199.1"
                     /translation="MQRSTKIVATLGPASSDREVLTRMIAAGVDVVRMNFSHGSAEEH
                     LARAELVREVARTVERTVGIMCDLQGPKIRIGKFEKGKVTLNKGEVFVLDAEVPLGNQ
                     ERVGLDYKELPRDVSPGSVLLLDDGRMVLDVQKVQGSQITTKVRVGGVLSNNKGINRQ
                     GGGLTAPALTAKDMEDIKTSVVMKADFLAVSFPKSGADMYMAKELMRAAGGRSTLIAK
                     MERAEAIAAMAEILEASDGIMVARGDLAVEVGDASVPALQKRMIRTARERNKLTITAT
                     QMMESMISSPVPTRAEVSDVANAVLDGTDAVMLSAETASGKYPVETVEAMARICLEAE
                     KSAEVTLDREFLDRVFTRVDQSIAMAALFTAYHLKVKAIAALTQSGSTALWISRLNCG
                     VPIYALTPEISTRYRLSLYRDVYPLLIKYVGHDRDHLLQEAEDQLVSAGLVQKGDLIV
                     ITFGEPIGAPGGTNTMKIVKVGESRKP"
     gene            7078..7587
                     /gene="purE"
                     /locus_tag="VE030_01905"
     CDS             7078..7587
                     /gene="purE"
                     /locus_tag="VE030_01905"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HZF22200.1"
                     /translation="MATRKTTAPLVSIVMGSTNDWDVMQHAARQLKDFGVPYEARALS
                     AHRTPELLAAYVKQCVEGGTQCFIAGAGGAAHLAGVIAALTTLPVLAVPIPSKHLGGL
                     DSLLSMVQMPKGIPVATFAIGDGGSANAGLFAVAILALNDAGLAKRLQAFRIKQADAV
                     KAAKLPELS"
     gene            7618..8496
                     /locus_tag="VE030_01910"
     CDS             7618..8496
                     /locus_tag="VE030_01910"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HZF22201.1"
                     /translation="MQPIHETSLKSLKLLHRGKVRDLYAVDAERLLIVQTDRLSAFDV
                     ILPTPVPGKGRVLTEMSNFWFRKLRHVIPNHLLDVAPEMLVAAAERGQVQGRAFVVRR
                     LQPLPIEAIVRGYIAGSGWKDYQKTQMICGIPLPAGLKEAQQLPRPMFTPSTKAAIGT
                     HDENIPFSEMEKLIGKDLAGQVRDKAIQLYSEAAAYATTRGIIIADTKFEFGRDANGK
                     LYLIDEALTPDSSRFWPKEQYRIGINPPSFDKQFVRDWLESQKWNKQPPAPALPADVL
                     KKTAEKYEEALRLLTA"
     gene            8719..>8913
                     /locus_tag="VE030_01915"
     CDS             8719..>8913
                     /locus_tag="VE030_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008652144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HZF22202.1"
                     /translation="MTTEQEIETAAEILRAGGLVAFPTETVYGLGADASSPAAVKKIF
                     AAKGRPADHPVIVHIADMSEL"
BASE COUNT         1819 a         2646 c         2866 g         1582 t
ORIGIN      
        1 ccccgagtac ggcatggcac cgggagtgga gaccactacc ggccctctcg gccagggtct
       61 tgccaatgcg gtcggcatgg ccatcgcgga aaaactgctc gccgccgaat tcaaccgtcc
      121 cggccatggc atcgtcgatc accgcaccta cgtcttcgtc ggcgacggtt gcctgatgga
      181 aggcatttcg cacgaagcct gttcgcttgc cggcacgctg ggcctgggca agctgaccgc
      241 ggtctacgac gacaacggca tttccatcga cggccatgtc agcggctggt tccgcgatga
      301 cacgccgaaa cgtttcgaag cctatggctg gcatgtgctg cgcaacgtcg acggccatga
      361 cgtggccgcg gtggatgcag cgatcatcca ggccgggcga gtcgcggaca agccgagtct
      421 gatctgctgc aagacggtga tcgccaaggg ctcgcccaac aaggccggca gccacgaagc
      481 gcatggcgca gcgctgggcg accaggaagt tgcggcgacc cgcgagaaca tcggctggaa
      541 gtatcccccg ttcgaaatcc cgcaggacat ctacgccggc tgggatgcgc gggcaaaagg
      601 cgctgaactg gaagccggct ggaaccggaa gttcgccgcc tacgccaagg cctttccgca
      661 ggaagccggc gagttcaccc gccgcgtgaa cggcgagctg ccgaaacagt ggcacacgca
      721 tacgcaggat ttgctcgcca aagtcaacgc taaggccgaa tccatcgcca cccgcaaagc
      781 ctcgcagaac gccatagaga atctggcgcc ggtgctgccg gagctcgtcg gcggttccgc
      841 cgatctcacc ggctccaacc tcacgctctg gtccggatcc aaggccgtgt ccagcgacag
      901 cggcggcaat tacatttatt acggcgtgcg cgagttcggc atgtccgcga tcatgaacgg
      961 gatgacgctg cacggcggcc tgatcgccta cggcggcacc ttcctgacgt tctccgatta
     1021 ctccaggaac gcgctgcgca tggcggcgct gatgaaaatc cgcagcctgt tcgtgttcac
     1081 ccacgactcg atcggcttgg gtgaggatgg ccccacccac cagtcggtcg agcattcggc
     1141 gagcctgagg ctgataccga acatgaacgt gtggcgtccc tgcgactcgg tggaatctgc
     1201 ggtggcgtgg attgcggcga tcgagcgccg ggacgggccg accagcctgc tgttctcgcg
     1261 tcagaacctg cctttccagg cgcgcagcgc ggaacagatc gcgctgatcg gacgcggagc
     1321 ttacgttctt gccgatgttg acaggccgcg ggcggtgatc atcgccacag gttcggaagt
     1381 ggcgctggcc atcggtgcga agaaggaact ggaaggacag gacatcgcgg tgcgcgtggt
     1441 ttcaatgcca tgtaccagcg ttttcgatcg tcaggatgca gcctacaaaa acgcggtcct
     1501 gcccaaggga gtcaaacgcg tcgcggtgga agcgggcgtc accgactact ggcgcaaata
     1561 cgtcgggctg gacggcgtgg tggtggggtt ggatcgcttt ggcgagtcgg cgcccgccgg
     1621 tgatctcttc aaatttttcg ggttcactgt cgagagcgtg gtcaaggccg tcaaaggcgt
     1681 actgtagcca caagccgggc ggcgcccgcg ccggtccgaa cgaggagtag cgatgggcaa
     1741 ggcggtgcgg ctggtcggtg acatcggcgg gaccaacgca cgtttcgcgc tggttgccga
     1801 cggctccagc cagccacagc aggagcaggt gatgcgctgc gccgagttcg agggcctcga
     1861 gcaggcgatt cgccgctacc ttgccgatca cggcaatccc gggattcgcg aagctgcgct
     1921 cgacgttgcc accggcatca ccggcgattt cgtgcagctc accaacggac cctggggatt
     1981 ctcgatcgag accacgcgcc aggttctcgc gctcgatcgg ttgcatgtga tcaatgattt
     2041 caccgcgctt gcgctgtccg tgcccacgtt gcagccgcac gagcttagac aggtcggcgg
     2101 cggtaggccg gtggcaggaa ccgcgatcgc ggtgatagga ccgggaaccg gtctgggcgt
     2161 atccggcctg cttcccatgg gcgagggctg gatggcggtg cagggggagg gcgggcatac
     2221 cgcattcagc ccgacgaccg agcgcgagat cgaggtcctg cgctggctga tgccgcaatt
     2281 cgagcatgtt tcgaccgaac gcgtggtgag cggcatgggg ctcgagaacc tctatcaggc
     2341 cttgtgcgcg ctggcccacg ccacggcgca gccgctaacg ccggcgcaga tcaccgaggc
     2401 cgcactggcc ggcaaagagg tgcattgccg cgaagcgctc gacatgttct gcgccatcct
     2461 gggcgcagcc gcggccaatc tggtagtaac gctcggcgct cgcgcgggat gctatatcgg
     2521 cggcggaatc gtgcccaggc tgggggaata ttttgcgcgt tcgccgttcc gtgcccgatt
     2581 cgagcgcaag ggccgtttct ccgcttacgt cgcggcagtg ccgacttatg tgatccttgc
     2641 ggagacgccc gcattacgcg gacttgcaag cttatttacg gacagaagat gaaatcatgg
     2701 tagcaatgac cgatgtcatc ggtctggtgg cgggattgct gactacgacg gccttcatac
     2761 cccaggtgct gaaaatctat aacaccaagt cgggcaaaga catttccagc aggatgattt
     2821 ccatgttcag catcgggctg gtgctgtggt tgatctacgg catcatcatt cgctcaaccc
     2881 cgatcatcgt caccaatgcg tttacgctga tcctggcgct cgcgatcatc gtgctgaaga
     2941 tccgctacag ccggttgcac tcccgcagcc ggcatgagtc cgggcgtccc tgaaccgcct
     3001 tgcagcggcc cgaatgggcc gccgtcgccg cagtgcatca cgcctgtagg gcttgggcaa
     3061 agtgaagtaa aatccgagct ttaagaggga gtttcgttta tgaccatccg cgttgcaata
     3121 aacggctacg gacgcatcgg cagaaatatc ctgcgggcgc actacgaagg cggcaggaag
     3181 catgacatcc ggatcgtcgc tatcaacgat ctcggcgacg caaaaattaa cgcccacctt
     3241 accaagtacg acaccgcgca cggcaagttt ccgggcagcg tctccgtaga tggggaattt
     3301 ctggtcgtca acggcgacaa gatcaaggtc tgcgcgcaac gcaacccggc cgagctgccc
     3361 tggaaggatc ttggcgcgga tgtggtgctc gagtgcaccg ggctctttac cagcaaggcc
     3421 aaatccggcg cgcatctgag cgcgggcgca aagaaggtca ttatctccgc gccgggcgac
     3481 aaggacgtgg acgcgaccgt ggtgtacggc gtaaatcacc atacgctgaa agcggcgcac
     3541 accgtgatct ccaatgccag ttgcaccacc aactgcctgg cgccgatggt gaaggcgctg
     3601 aacgaccgca tcggtgtggt caacggcctg atgaccacca tacacgccta caccaacgac
     3661 caggtgctga ccgacgtgta tcacaccgac ctgcgccggg cgcgctcggc gaccatgtcg
     3721 atgattccga ccaagacagg cgccgcagca gcggtgggcc tggtgctgct ggaactgaac
     3781 ggcaagctgg acggtttcgc gattcgcgtg ccgaccatca atgtatcgct ggtggatctc
     3841 gcctttgtcg ccaagcgcgc gaccacggtc gaagaggtga acaacgcggt gaagcaggcc
     3901 tcggagtccg acctcaaggg catcctggag tacaacaagg atcccctggt gtcggtcgat
     3961 ttcaaccaca acccggcgtc ttcggtgttc gactcgacgc tcaccaaggt ttcggaaggc
     4021 acgctggtga aggtatgctc ctggtatgac aatgagtggg gcttcagcaa ccgcatgctg
     4081 gatgtcacgg ttgcgcttgc gaacgcgaag taaacagaag catttaccgc agcgggcgcg
     4141 gagaaaacaa aggccggaaa tttcaggggg tcttttttct gcgccctttg agttctctgt
     4201 ggcggaaagg tgttgactag agaccatgac gatcctgcgc atgaccgatc tcgatctgcg
     4261 cggcaagcgc gtgttcattc gcgctgatct gaacgtgccg caggacgccg ccggcaacat
     4321 taccgacgac acccgcatcc gcgcctcggt accggcgatc aggcacgcgc tgaactccgg
     4381 cgccgccgtc atggtgacct cacacctggg ccggccggcc gagggcgagt tgaagcccga
     4441 ggattcgctc gcgccggtgg caaagcgatt gtcggaactg ctcggcgtgc cggttccgct
     4501 caagacgaac tgggtcgagg gcgtggacgt gaaacccggc caggtcgtgc tgctggaaaa
     4561 ttgccgggtc aacaagggcg agaagaagaa cgacgagacc ctggccagaa aaatggcggc
     4621 gctgtgcgac atttacgtga acgacgcatt cggcaccgcg catcgagccg aggcgtcaac
     4681 ccacggcatc gccaggtttg caccggtcgc ctgtgccgga ccgctgatgg cggcggagct
     4741 ggaagctttg ggcaaggcgc tgaccgtgcc gaaacggccg ctggtcgcga ttgttgcggg
     4801 ctccaaggtc tctaccaagc tcagcatcct gggcgagctg gcgaaaaaag tggatcaact
     4861 gattgtcggc ggcggtatcg ccaatacatt tatgttggcc gagggcctgc cggtcggcaa
     4921 gtcgctgatc gaagccaatc tggtcggcga ggcgaaaaga atcatcgaag cgatgagggc
     4981 gaagggcggg gctgttccga tcccggagga cgtggtgtgc gccaaagaat tttccgcgca
     5041 cgccaaggcc atggtgaaag acgcgggcga cgttgcggcc gatgacttga tcctcgacat
     5101 cggcccaaag accgccgggc ggctcgctga cgcgctcggc aaggccggta cgatcgtctg
     5161 gaacggcccc gtgggcgtgt tcgaattcga ccagttcagc gaggggacga aaaccatagc
     5221 gcacgcgatc gcggcttccc cggcattctc gatcgccggt ggcggcgaca cggtcgccgc
     5281 aatcgccaag tacgggatcg agaagaaaat cggctacatt tccaccggcg gcggcgcttt
     5341 cctggaattc ctggagggca agaaacttcc ggcagtggat attctcgaac agcgggctat
     5401 tgcgagaatc tgattgacca cagagacaca gagataaatc acgagatgca caatgagaga
     5461 gcaagaaggg gcgccacgaa atcatgttct cgttttgtaa tgctttcttt ttcggtctaa
     5521 cagtttttct ctgtgtctct gtggtgaaaa ttcaatgcaa agaagcacaa aaatcgtagc
     5581 caccctcggc ccggcatcga gcgaccgcga agtgctgacg cgcatgatcg cagccggcgt
     5641 cgacgtggta cgcatgaatt tctcccacgg cagcgcggag gaacacctgg cgcgcgcaga
     5701 actggtacgc gaggtggcgc gcaccgtgga gcgcacggtc ggcatcatgt gcgacctgca
     5761 gggaccgaag atccgcatcg gcaagttcga gaagggaaaa gtcaccctca acaagggcga
     5821 ggtattcgtc ctcgatgccg aggttccgct cggaaatcag gaacgcgtgg gcctggatta
     5881 caaggaactg ccgcgcgacg tctcgccggg gtcggtgctg ctgctggacg acggccggat
     5941 ggtgctcgac gtgcaaaagg ttcagggcag ccagatcacc accaaagtac gcgtgggtgg
     6001 cgttctctcg aacaacaagg gcatcaaccg ccagggcgga ggactgacgg cccccgcgct
     6061 gaccgccaag gacatggagg acatcaagac ctcggtggtg atgaaggcgg atttcctggc
     6121 ggtgtccttc cccaagagcg gcgccgacat gtacatggcc aaggagctga tgcgcgcggc
     6181 cggcggccgc tcgacgctga tcgccaagat ggaacgcgcc gaggcgatcg ccgccatggc
     6241 ggagatcttg gaggccagcg acggcattat ggtggcgcgc ggcgatcttg ccgtggaagt
     6301 cggcgatgcc tcagtgccgg cgctgcagaa acgcatgatt cgcaccgcgc gagaacgcaa
     6361 caagctcacc atcaccgcaa cgcagatgat ggagtcgatg atctcgagcc cggtgccgac
     6421 gcgcgcggag gtttccgatg tggcgaacgc ggttctcgac ggtaccgatg ccgtgatgct
     6481 ctccgccgag acggccagcg gaaaataccc ggtggaaacc gtcgaggcga tggcgcgcat
     6541 ctgcctggag gcggagaaat ctgccgaggt gaccctggac cgcgaattcc tcgatcgcgt
     6601 gttcacccgc gtggaccagt cgatcgcaat ggcggcgctt tttaccgcgt atcacctgaa
     6661 agtcaaggcg atcgcagcgc tgacccagtc cggctccacc gccctgtgga tttcgcgcct
     6721 gaactgtggg gtgccaatct acgcattgac accggagatt tccacgcggt atcggttgag
     6781 cctgtaccgc gatgtctatc cgctgctgat caaatacgtc ggccacgacc gcgaccacct
     6841 gctacaggag gcggaagacc aactggtctc cgccgggctg gtacagaagg gcgacctgat
     6901 cgtgatcact ttcggcgagc cgatcggggc gcccggcggc accaatacca tgaagatcgt
     6961 caaggtgggc gagagccgca agccgtaggt gggtgaggcg agaggcgtga ggcgagatga
     7021 tcgttgtcgg gatcatcggg cttaaaatta cttcgtccga ctagttcagg agcgaccatg
     7081 gcaaccagga aaacgaccgc gccgctcgtc tcgatcgtga tggggagcac caacgactgg
     7141 gacgtaatgc agcacgcggc gcggcagttg aaggacttcg gcgtgcccta cgaagcgcgg
     7201 gcgttatccg cccaccgcac tccggaactg ctcgcggcgt atgtgaaaca atgcgtggag
     7261 ggcggaacgc agtgttttat cgccggtgcc ggcggtgccg cgcatctcgc cggggtcatc
     7321 gctgcgttga ccaccctgcc ggtgctggcg gtgccgattc cctccaagca cctcggaggc
     7381 ctcgactcgc tgctgtccat ggtgcagatg ccaaagggca ttccggtcgc gacgtttgcg
     7441 atcggcgacg gcggctcggc caacgccgga ttgtttgcgg tggcgatact agcgctgaac
     7501 gacgctggat tggcgaagcg cctgcaggcg ttccgcatca agcaggcgga tgcggttaaa
     7561 gcggcaaagc tgcccgagct atcctgatcg aaacgtagcg aaatcatgat aagtgcaatc
     7621 caacccattc acgaaaccag cctgaagtcg ctcaagctgc tccatcgtgg caaggtgcgc
     7681 gacctctacg cggtggacgc cgagcgtctg ttgatcgtgc agaccgaccg cttgtcggct
     7741 ttcgacgtga ttcttcccac cccggtaccg ggcaagggcc gggtactcac cgagatgtcg
     7801 aacttctggt tcaggaagct ccgccacgtg atcccgaatc acctgctcga cgtggcgccc
     7861 gagatgctgg tcgcggcggc ggagcgcggc caggtacagg ggcgcgcctt cgtcgtgcgg
     7921 cgcttgcagc cgctgcccat cgaagcaatc gtgcgcggct acatcgccgg ctcgggctgg
     7981 aaggattacc agaagaccca gatgatctgc ggtatcccgt tgcccgccgg gctgaaggag
     8041 gcgcaacaac tgccccggcc gatgtttacg ccttccacca aggccgcgat cggcacgcac
     8101 gacgagaata ttcctttctc ggagatggag aagttgatcg gcaaggacct ggcgggacag
     8161 gtacgcgata aggcaatcca gctctattcc gaagcggcgg cgtatgccac cacccgcggc
     8221 atcatcattg ccgacaccaa gttcgagttt ggccgcgatg ccaacggcaa gctgtacctg
     8281 atcgacgagg cgttgacgcc ggattcatcg cgcttctggc cgaaggagca gtaccggatc
     8341 ggcatcaacc cgccctcgtt cgacaagcag ttcgtgcgcg actggctgga atcgcagaaa
     8401 tggaacaagc agccgccggc gccggccctg cccgccgatg tgctcaagaa gaccgctgag
     8461 aaatacgaag aagcgctgcg gcttttgacc gcataaagac gaaacgcggt taacacgaag
     8521 ggtcacaaag gaagagcaag aaaggaacac gaaggaaaac aaaaatcgag caagaatcag
     8581 gaagtatgga ttcgagtatc gttcctgtat tcctatgcag cttttgggtc tttgcgttga
     8641 aagcttttga ctttgatctc cttcgtgtac cttcgtattt ccttcgtgac ccctcgtgtt
     8701 aaaagaggtt gttcctaatt gacgactgaa caggaaatcg agacagcggc agagatcctc
     8761 cgcgcgggag ggctggtggc gtttccgacc gagaccgtgt acgggctcgg cgcggatgcg
     8821 tccagtcccg ccgcagtgaa gaagatcttc gccgccaaag gccgtcccgc cgaccatccg
     8881 gtgatcgtcc acatcgccga catgagcgaa ttg
//
LOCUS       DASQOP010000052        12497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_421291, whole genome shotgun sequence.
ACCESSION   DASQOP010000052 DASQOP010000000
VERSION     DASQOP010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12497
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421291"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..1185)
                     /gene="obgE"
                     /locus_tag="VE030_01920"
     CDS             complement(124..1185)
                     /gene="obgE"
                     /locus_tag="VE030_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HZF22203.1"
                     /translation="MKFVDEATIEVHAGKGGDGIASFRREKFIPRGGPDGGDGGRGGS
                     IHAVADRNINTLVEYRYARIHRARNGGKGQGSDCYGKAADDITLQVPVGTVISDLASG
                     EVIADLSVDGQAAVLVNGGQGGLGNIHFKSSTNRAPRQCTPGEPGENRTLKLELKVLA
                     DVGLLGLPNAGKSTFIRSVSAARPKVADYPFTTLHPNLGVVRVDDNRSFVVADIPGLI
                     EGAAEGAGLGHQFLRHLQRTRLLLHLVDLAPLDDGADPVHGARAIVEELRKYDESLYR
                     KPRWLVLNKADLIAPEDREEKVRAFLSEYARAVAAPEKSFIISALTGEGCRALTYAIM
                     EHLQHHARVEPAEAAEDAE"
     gene            complement(1420..1677)
                     /gene="rpmA"
                     /locus_tag="VE030_01925"
     CDS             complement(1420..1677)
                     /gene="rpmA"
                     /locus_tag="VE030_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HZF22204.1"
                     /translation="MAHKKAGGSSRNGRDSQSKRLGVKRYGGEVILAGSIIVRQRGTQ
                     VHAGENVGMGKDHTLFAKIDGKVTFAVKGPDKRKVVSVTPA"
     gene            complement(1690..2001)
                     /gene="rplU"
                     /locus_tag="VE030_01930"
     CDS             complement(1690..2001)
                     /gene="rplU"
                     /locus_tag="VE030_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HZF22205.1"
                     /translation="MYAVIKTGGKQYRVSSGEKIKVEQIPADVGSQISLDQVLMVADG
                     DKVSIGAPLVAGATVLATVVAHGRADKVHIFKMRRRKHYRKHGGHRQNYTEIQIDKIS
                     G"
     gene            2205..3173
                     /gene="ispB"
                     /locus_tag="VE030_01935"
     CDS             2205..3173
                     /gene="ispB"
                     /locus_tag="VE030_01935"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HZF22206.1"
                     /translation="MTPHLINDLIAADLRAVDEVIRKRLDSEVVLVRQVADYIIGGGG
                     KRLRPALVILAAGACGYTGSHHLQLAAVVEFIHTATLLHDDVVDDSQMRRGRATANSA
                     FGDAAAVLVGDFLYSRAFQMMVQAQSMRVMEVLADATNTIAEGEVMQLLHVHNADVTE
                     TDYLRVIRSKTAKLFEAAGRLGGILAGVPRAQEDALAEYGMHLGTAFQLVDDILDYSG
                     DLAQTGKNLGDDLSEGKATLPLIRAMRNSGAAQSALIRKALVEGGLSDFGPVMRAMQE
                     TGALEYTRRQAEDSARQARTAIAGLPHSKYKDCLLELAAFAVTRNY"
     gene            3195..3271
                     /locus_tag="VE030_01940"
     tRNA            3195..3271
                     /locus_tag="VE030_01940"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3229..3231,aa:Pro,seq:cgg)
     gene            3300..3539
                     /locus_tag="VE030_01945"
     CDS             3300..3539
                     /locus_tag="VE030_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HZF22207.1"
                     /translation="MAKFLLVIAVFIVVYLFLRSYRGRAEAAPPPAAAAKHGEDMVRC
                     LICGVHLPKSEAVTSRGQIYCSKEHLQLADRERGR"
     gene            3559..5172
                     /locus_tag="VE030_01950"
     CDS             3559..5172
                     /locus_tag="VE030_01950"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZF22208.1"
                     /translation="MSSVLSFPQISSVERRQYGDAFWRSLFYFNVYRLAVAITLMAAV
                     MTFGDNIPFGSFSRRTFLYANIGYIVFSLLSFASVIPRKPYFTVQLTVQICGDILFTV
                     LLMYASGGIGSGLGLLLLASLAAVGLISRGRLTLFFAALATIAVLAEHTYQVVFQNGS
                     TSQFAQAGLLCIGFFATGWLAHSLAKRAVVIEAIAAQREVDLANMAQVNQLVIQDMQD
                     GLLVVDEHGAIRQINGQAEAILGPLRRSPREVLLRDYAPTLAARLEQWRRDPKTNFEP
                     LRTALTHKPTAARFVPVGISRNVGVVIFLEDLSRIQQQAQQLKLASLGRLTANIAHEI
                     RNPLSAINHATELLLEESGFRGQPHGRMLQIIHDNTQRLDRMVQDVLKLNRRDQATRE
                     HFKVGEYLHTFVSEFSEIEKIPGDIILIDIEGDPTVAFDRSHLNQVMWNLCRNALRYC
                     RKQAGSILLRVRPGPRQSTIELSVIDDGTGVAEALRGHLFEPFFTTASSGTGLGLYIA
                     REICEANGATLEYVDRPVGAEFTLICRGT"
     gene            5175..6539
                     /locus_tag="VE030_01955"
     CDS             5175..6539
                     /locus_tag="VE030_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZF22209.1"
                     /translation="MKRKEQTAGANSRSVLIVDDEEDILELIELTLLRMGLDVDRATT
                     VKMAIEKVQSGNYDLCLTDMRLPDGEGLQLVQFISDNLSDLPVAVITAHGNTENAVAA
                     LKAGAFDYLAKPVSLEQLRTLVKSALSLPQATAAKAATGHALLGESGAIQQVRELIEK
                     LARSEAPVHISGESGSGKELAARLIHEKSARRSQPFMPVNCGAIPENLMESEFFGYRK
                     GAFTGADADRDGFFQAANSGTLFLDEVADLPLAMQVKLLRAIQEKKVRKVGSTQEEPV
                     DVRIISATHQKLGECVEAGKFRHDLYYRLNVIELRMPALREMREDIPQFVSALLDKLA
                     RNAGTKKPDVVSEALAELERYDFPGNVRELENVLERAMALCSDARITAGDLQLTPHDL
                     PEQVVATGGDSKAPLQDYLDRVEKEAILEALEKTRYNRTAAAKLLGITFRSMRYRMER
                     LGLN"
     gene            6569..7126
                     /gene="ampD"
                     /locus_tag="VE030_01960"
     CDS             6569..7126
                     /gene="ampD"
                     /locus_tag="VE030_01960"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HZF22210.1"
                     /translation="MDSWKLGTSGLLSAAAYVPSPNCDDRPGGANVELLVIHNISLPP
                     GEFGGPGIVELFTNHLDPRAHPYYREIQGLKVSSHFLIRRDGELIQFVPCIRRAWHAG
                     QSAWRGRTLCNDFSVGVELEGADDQPFADSQYAVLAELTRALQRAYPIRDCVGHSDIA
                     TPPGRKTDPGPHFDWARFRSLIKGE"
     gene            7213..7890
                     /locus_tag="VE030_01965"
     CDS             7213..7890
                     /locus_tag="VE030_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HZF22211.1"
                     /translation="MKIFYTDHYVLPLPAGHQFPMAKYSLLRARHAFRDHGEGYCVFN
                     DSAIAALSMQAEGCARRVAILDCDLHRGNGTASILADEPTIFTFSIHAAKNFPFRKEA
                     SDLDIEMPDGVGDADYLDALEQGICHALAAAQPDLAIYLAGADPYVGDRLGRLSLTKE
                     GLQARDRKVFELCRAASVPVAVAMTGGYSNDIADSVDIHFCTVETAASFWRAAQRPTS
                     LPAARQI"
     gene            8139..11009
                     /locus_tag="VE030_01970"
     CDS             8139..11009
                     /locus_tag="VE030_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HZF22212.1"
                     /translation="MQLATNLTAATPVGNYSTEDGAADRDPQRYAQYKVIRRNGAVVG
                     FEPGKISVAVTKAFLAVNGGQGAASARVRELVEKLTETVVNALVRRQPNGGTFHIEDI
                     QDQVELSLMRSGEHEVARAYVLYREARARERARNRETQAAATAHAISVIEHGQTHPLN
                     MERLTGLIKDSCEGLGRAVGPAVILQMTLKDLYDGVPMDEVRKCAILAARSLIEKDPA
                     YSYVTARLLLNSVRYEVLGEEVTQGQMHSRYAEYFPGFIKRGIDAGLLDERLGRFDLR
                     SLGAALDANHDLKFGYLGLQTLYDRYFLHVHGQRIELPQAFFMRVAMGLAINEVEREA
                     RAIEFYNVLSRFDFMSSTPTLFNSGTHRSQLSSCYLTTVSDNLEGIYDAIKENALLSK
                     FAGGLGNDWTPVRALGSHIKGTNGKSQGVVPFLKVVNDTAVAVNQGGKRKGAVCSYLE
                     SWHLDIEEFLELRKNTGDDRRRTHDMNTANWIPDLFMKRVMEAGEWTLFSPADVPDLH
                     DKVGKEFEKAYTEYERKTQTGELKLFKRIPVLTLWRKMLTMLFETGHPWITFKDPCNI
                     RSPQSHVGMVHSSNLCTEITLNTSDSEIAVCNLGSINLVAHMKEGKLDHDKLKQTVST
                     AMRMLDNVIDVNYYAVNKARNANFKHRPVGLGIMGFQDCLLEMRVPYASKEALDFADR
                     SMEAVAFYAYWASTELAEERGRYSTYEGSLWSRGILPQDSLKLLAEERGGYVEVDSSS
                     RMSWEPLRARIKQFGMRNSNCLAIAPTATIANIVGVSASIEPTYQNLYVKSNLSGEFT
                     VTSEYLVRDLKKLNIWDEVMIADLKYFDGSLAKIDRVPQEVRNLYATAFEVDSAWIVE
                     AGARRQKWIDQAQSLNIYMAGASGKKLDDIYKLAWKRGLKTTYYLRTLGATSAEKSTA
                     RGGQLNAVPTDGGVMGAGSTAASADEETKFCSIDNPECEACQ"
     gene            11325..12407
                     /locus_tag="VE030_01975"
     CDS             11325..12407
                     /locus_tag="VE030_01975"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HZF22213.1"
                     /translation="MPAGNAPPARVAESGPSADVAFRRVRVEDKLVINGKADVNQLVP
                     FKYKWAWDKYLSGCANHWMPQEINMSRDIATWKDPNGLTEDERLVVKRNLGFFVTADS
                     LAANNIVLGTYRHITAPECRQYLLRQAFEEAIHTHAYQYIVESLGLDEGEIFNAYHEV
                     SSIRDKDEFLIPFIETLTDPEFKTGTPAADQQLLKSLIVFACIMEGLFFYVGFAQILA
                     LGRQNKMTGAAEQYQYILRDESMHCNFGIDLINQIKMENPNLWTPEFREEIRGLIQRG
                     VELEYRYAEDTMPRGVLGLNAPMFKEYLRFVANRRAQQIGLDPLFPGATNPFPWMSEM
                     VDLKKEKNFFETRVIEYQTGGALNWD"
BASE COUNT         2411 a         3891 c         3806 g         2389 t
ORIGIN      
        1 cagcgcggcg tggtccagcc cctgaccttc attggtgacc aggctgctgc cgaccttgac
       61 gaccaggcgt tgcgcttcgg cggcgacgga tttcatgaga tgaccgcgac ggtcccggcg
      121 atgctattcg gcgtcttctg cggcttcggc cggctcgacg cgggcgtggt gctgaagatg
      181 ttccatgatt gcgtaggtca gtgcgcggca accttctccg gtgagcgccg agatgataaa
      241 gcttttttcg ggcgcagcga cggcgcgcgc gtattcggac aggaaagccc ggactttttc
      301 ctcgcggtct tcaggcgcga tcaggtccgc cttgttgagc accagccagc gcggcttgcg
      361 atagagcgac tcgtcgtact tgcgcagttc ttcgacgatt gcgcgcgcgc cgtgcaccgg
      421 atcggcgcca tcgtcgagcg gcgccagatc caccaggtga agcaacaggc gcgtgcgctg
      481 caagtggcgc aagaattgat ggccgagccc ggcgccttcg gccgcgccct cgatcagtcc
      541 ggggatgtcg gcgaccacga agctgcgatt gtcgtccacg cgtaccacgc ccagattcgg
      601 gtgcagtgtc gtgaacggat agtcggcgac cttgggacgc gcggccgaaa ccgagcggat
      661 aaaagtggac ttgcctgcgt tgggcagacc cagcaacccg acgtcggcga gcaccttgag
      721 ttcgagcttg agggttcggt tttcgcccgg ctctcccggc gtgcattggc gcggcgcgcg
      781 gttggtgctc gatttgaaat ggatgttgcc gagccctccc tggccaccgt tgacgagcac
      841 ggcagcctgt ccgtctacgg acaggtcagc gattacttcg ccgctggcga ggtcgctgat
      901 tacggtgccc accggcacct gcagtgtgat gtcgtcggcc gccttgccgt agcaatccga
      961 accctggcct ttccctccgt tgcgggcacg gtggatgcgt gcgtagcggt actccaccaa
     1021 ggtgttgatg ttgcgatcgg ccacggcgtg gatgctgccg ccgcggccgc cgtcaccacc
     1081 gtccggcccg ccgcgtggaa tgaatttctc gcgcctgaac gaggcgatgc catccccgcc
     1141 cttgccggcg tggacttcga tggtggcttc gtctacgaat ttcatggaag ttcagtatca
     1201 ccacagagac acagagaagt gcaaaaaacg caaatacgcc cgtgaaacat cgacagatcg
     1261 gcttccggag gatggccggc gatcgcagcc cgctccttgc attggaaaaa ccattctttc
     1321 gcttctctcc gtgcctctgt gtctctgtgg taaatcggtc attcaaaaaa caaaagccct
     1381 accgggtccg atagggcttc ttgctgaaag cgggaaacct tacgccggcg tgacgctgac
     1441 gactttcctc ttgtccgggc ccttcaccgc gaaggttact ttgccgtcga tcttggcaaa
     1501 cagggtgtga tccttgccca tgccgacatt ttctccggcg tgaacctggg ttccgcgctg
     1561 acgcacgatg atgctgcctg ccaggatgac ctcgccgcca tagcgcttca cgcccaggcg
     1621 cttggattgg gaatcgcgac cgttgcgcga gctgccgcca gcttttttgt gtgccatatt
     1681 ctctgctcct cagcccgaga tcttgtcgat ctggatctcg gtgtaattct gccgatgacc
     1741 gccgtgcttt cgatagtgct tgcggcggcg catcttgaag atatgcactt tgtcggcgcg
     1801 gccgtgcgca acaaccgtcg ccagcactgt tgcgccggct accagcggcg ccccgatcga
     1861 gactttgtcg ccgtcggcga ccatcaaaac ctgatccagg ctaatctggc tgcccacgtc
     1921 tgcaggtatc tgttctacct tgattttttc gccggaagag acacggtact gcttgccccc
     1981 ggtttttatg accgcataca tgatggagcc ctcgatacgc ttttgaggaa agccgcgcat
     2041 actacataaa aagaaaggct tagtaaagcc gatggaagga tggccggaca cggatcaccg
     2101 gcccgggcgc actctctaga cagggatgat tggattcttg acaccgtagc tctgcgcttc
     2161 ctaccatcgc gcacttttga tttcgcagtt cccaggccgg ccacgtgaca ccccacctca
     2221 taaacgatct cattgccgca gacctgcggg cggtggacga ggtcatccgc aaacgactcg
     2281 attccgaagt cgttctggtg cgccaggtcg cggattacat tatcggcggc gggggcaagc
     2341 gactgcgtcc cgccctggtc atcctcgcgg cgggcgcctg cggatacacc ggcagtcacc
     2401 accttcaatt ggccgcggtg gtcgagttca tccataccgc cacgctgctt cacgacgacg
     2461 tagtcgacga ctcgcagatg cgacgcggac gcgccaccgc taattccgcg ttcggcgacg
     2521 ctgccgccgt gttggtcggc gattttcttt actcgcgcgc ctttcaaatg atggtgcaag
     2581 cgcagagcat gcgtgtgatg gaagtgctcg ccgatgccac caatactatc gccgagggcg
     2641 aggtgatgca gttgctgcac gttcacaacg ccgacgtcac cgagacggat tatctgcgcg
     2701 tgatccgctc gaagaccgcc aagctgttcg aggccgccgg ccgcctgggc ggtattctcg
     2761 ccggcgttcc gcgcgcgcag gaggatgcac tggcggagta tggaatgcac ctgggcactg
     2821 catttcagct ggtggacgac attctcgatt attcgggcga tcttgcgcag accggaaaga
     2881 atctgggcga tgacctgtcc gagggcaagg caacgctgcc gctcattcgc gccatgcgca
     2941 acagcggcgc cgcgcagagc gcgctcatcc gcaaggccct ggtcgagggc gggctgagcg
     3001 atttcgggcc ggtaatgcgg gcgatgcagg aaacgggcgc gctcgagtac acgcgccggc
     3061 aggcggaaga ctcggcgcgg caggcgcgca cagccattgc cggcctgccg cattctaaat
     3121 acaaggattg tttgctagaa ttggcggcct ttgcggtgac ccgcaattac tgaagcgtta
     3181 acgaggctca ccgtcggggt gtagctcagc ctggtagagt actgcgttcg ggacgcagga
     3241 gtcggaggtt cgaatcctct caccccgacc attccacatc accccacttc ggagcccgcg
     3301 tggccaagtt tctcctagtc atcgccgtct tcatcgtggt gtacctgttc ctccgcagct
     3361 atcgcgggcg cgccgaggcg gctccgccgc ccgcggccgc ggcaaagcac ggggaggaca
     3421 tggtgcgctg cctgatctgc ggcgtgcacc tgccgaagag cgaagccgtc acctcccgcg
     3481 gccagatcta ttgcagcaag gaacatctcc aattggccga tcgggaacgc ggccgctgac
     3541 ctgcgcgtgc ccgcgccgat gagcagcgtc ctgtcctttc cccagatttc atcggtcgag
     3601 cgccgccagt atggcgacgc gttctggcgc tcgctcttct atttcaatgt ctatcgcctt
     3661 gccgtcgcaa tcacgctgat ggcggcggtg atgacgttcg gcgacaacat tcccttcggc
     3721 tccttcagcc gcaggacctt cctgtacgcg aacatcggtt acatcgtctt cagcctgttg
     3781 tcttttgcgt cagttattcc gcgcaaaccg tatttcaccg tgcagctcac ggtgcaaatc
     3841 tgcggggaca ttctctttac cgttctgctc atgtacgcga gcggcgggat cggcagcggc
     3901 ctgggcctgc tgttgctcgc gtcgctggcc gctgtcggcc tgatcagccg cgggcgcctg
     3961 acgctgtttt tcgcggctct ggcgacgatt gccgtgcttg ccgagcacac ctatcaagtg
     4021 gtgttccaga acggatcgac ttcccagttc gcgcaggctg gcctgttgtg catcggattc
     4081 tttgccaccg gctggcttgc gcactcgctt gccaagcgcg ccgtggtgat cgaggcgatt
     4141 gcggcacagc gcgaggtgga tcttgccaac atggcgcagg tgaatcagct ggtgatccag
     4201 gacatgcagg acgggctgct ggtggtggac gagcatggtg ccattcgcca gatcaacggc
     4261 caagcagaag ccatactggg accgttgcgc cgaagtcccc gcgaggtgct gctgagggac
     4321 tatgcgccta cactggctgc gcggctggaa cagtggcgcc gcgatccgaa gacaaacttc
     4381 gagccgctgc gcaccgcgct gacccacaag ccgaccgctg cccgattcgt gccggtgggc
     4441 atcagccgca acgtgggcgt agtgatcttc ctggaggacc tctcgcgcat ccagcagcag
     4501 gcgcagcagt tgaagctggc gtcgctgggc cggctgaccg ccaatatcgc ccacgagatc
     4561 aggaaccctc tgtcggcgat caaccacgct accgagttgc tgcttgagga gtcgggattt
     4621 cgcggccaac cgcatggccg gatgctgcag atcattcatg acaacacgca gcggctggac
     4681 cggatggtgc aggacgtgct caagctgaac cggcgtgacc aggccacgcg cgaacatttc
     4741 aaggtgggcg agtatctgca caccttcgtt tccgagttct ccgagatcga gaagattccg
     4801 ggcgacatca ttctcatcga catcgaagga gacccgaccg tggccttcga tcgcagccac
     4861 ctcaatcagg tgatgtggaa cctgtgcagg aatgccctgc gatactgcag gaaacaagcc
     4921 ggcagcattc tgttgcgcgt gcggccgggt ccgcgacaga gtacgataga gttgagtgtc
     4981 atagatgacg gaaccggagt ggcggaggcg ttgcgcgggc atctgttcga gccattcttc
     5041 accacggcct ccagcggcac cggtcttggg ctctacattg cccgtgagat atgtgaagcc
     5101 aatggcgcca cgctcgaata cgtggatcga ccggtcggcg cggagttcac cctgatctgt
     5161 agaggaacat gagcgtgaaa cgcaaggagc aaacggcggg cgccaactcg cgctcagtgc
     5221 tcatcgtcga cgacgaggaa gacatcctcg agctcatcga gcttaccctg ctgcgcatgg
     5281 gcctggatgt cgaccgtgcc accaccgtaa agatggcgat cgagaaggtc cagtccggca
     5341 attacgacct ttgcctcacc gacatgcggc ttcccgacgg cgagggcctg cagttggtgc
     5401 agttcatctc cgacaatctt tccgacctcc cggtcgcggt gattaccgcc cacggcaata
     5461 ccgagaacgc agtcgcggcg ctcaaggccg gcgccttcga ttacctggcc aagccggtgt
     5521 cgctcgagca attgcgcacg ctggtgaaat ccgcgctgtc gttgccgcag gccaccgcag
     5581 ccaaggccgc gaccgggcat gcgcttctcg gcgagtcggg cgcgatccag caggtgcgcg
     5641 agttgatcga gaagctggcg cgcagcgagg cgccggtgca catcagcggc gaatccggca
     5701 gcggcaagga gcttgcggcg cggctcatcc acgaaaaaag cgcgcgccgc agccagccct
     5761 tcatgcccgt caattgcggc gcaatccccg agaacctgat ggagtccgag ttcttcggct
     5821 accgcaaggg cgcgttcacc ggcgcggacg ccgaccgcga cggttttttc caggctgcca
     5881 acagcggcac cctgtttctc gacgaggtgg cggacctgcc gctggcgatg caggtgaaac
     5941 tcctgcgcgc gatacaggag aagaaggtac gcaaggtggg ctccacccag gaagagccgg
     6001 tggacgtgcg catcatcagt gccacccacc agaaactcgg cgaatgtgtc gaggcgggca
     6061 agttccggca cgacctttat taccggctca acgtcatcga attgcgcatg ccggcgctgc
     6121 gggagatgcg cgaggacatc ccgcagttcg tgagcgcgtt gctggataag ctggcccgta
     6181 atgccgggac aaaaaagccg gacgtagtca gcgaggcttt ggcggaactc gagcgctacg
     6241 acttccccgg caacgtgcgc gaactggaaa acgtgctgga acgcgcgatg gcgctatgca
     6301 gcgacgccag gatcaccgcc ggggacctgc aactcacccc gcacgacctg cccgagcagg
     6361 tcgtggccac cggcggtgac agcaaggctc cattgcagga ctatctcgac cgcgtggaga
     6421 aggaagccat cctcgaggcg ctggagaaaa cccgatacaa ccgcactgcc gcggccaagc
     6481 tcctcggtat caccttccgc tccatgcgct acagaatgga acggcttggt ctgaactagt
     6541 gaggccggcg gcgggcgggg ggaagtgggt ggattcctgg aagctcggca cttccggtct
     6601 gctttccgcg gcggcttacg tgccttcacc gaactgcgat gaccggccgg gaggcgcgaa
     6661 cgtcgagctc ctggtcattc acaacatcag cctgccgccg ggcgagttcg gcggccccgg
     6721 catcgtcgaa ctgttcacca accacctcga cccgcgtgcc catccgtact accgcgagat
     6781 tcagggcctc aaggtttcca gtcacttcct gatacgccga gatggcgagc tgatccagtt
     6841 cgtcccgtgt atccggcgcg cctggcatgc cgggcagtcc gcgtggcgcg gccgcaccct
     6901 ctgcaacgac ttctcggtcg gcgtcgagct cgaaggcgct gacgaccaac cgttcgccga
     6961 ttcccagtac gcggtactgg cggaactgac ccgggcgctc caacgcgcct acccgatccg
     7021 ggactgcgtg gggcattccg acatcgccac cccgcccggt cgcaagaccg accccggacc
     7081 gcatttcgac tgggcccgct tccgctcact gataaaaggc gagtgaatga gtgaagagtg
     7141 aatgcgtaag ggccatcctg ttctggccat gctttttacc cttcactttt cacgccttca
     7201 cccattcacg ccgtgaaaat cttctacacc gaccactacg tgctgccgct gccggcggga
     7261 caccaatttc cgatggcgaa gtactcgctt ctgcgcgctc gccatgcctt tcgcgaccat
     7321 ggcgagggct attgcgtgtt caatgattcc gccattgcgg cattgtcgat gcaagccgaa
     7381 ggctgcgccc ggcgggtcgc gatcctcgac tgcgacctgc accgaggtaa cggcaccgcc
     7441 tcgatcctgg ccgacgagcc aaccattttc accttctcga tccacgccgc gaagaatttc
     7501 ccgttccgca aagaggcgag cgacctggac atcgaaatgc cggatggcgt cggcgatgcc
     7561 gactacctcg acgcactgga acagggcatc tgccatgcgc ttgccgcggc gcagccggac
     7621 ctggcgattt atcttgccgg cgccgatccg tacgtgggcg accggctcgg acggctttcg
     7681 ttgaccaaag agggtctgca ggcgcgtgac cgcaaggtgt tcgaactgtg ccgggcggcc
     7741 tcggttccgg tggccgtggc gatgaccggc ggctattcca acgacatcgc ggactccgtg
     7801 gatatccact tctgcaccgt cgaaaccgct gccagtttct ggcgcgcggc gcaacgtccg
     7861 acttctcttc ccgcggcgcg gcagatctga agccgaacag gcgtcgcagc gccgcgtcgc
     7921 tgccgcgttt cgtcacttcg cgtgcacttc aactgcgcga ccgagcattt tgtttttcaa
     7981 atgtaaaaaa ttcttgacag tgggtgtggt gctactagaa ttagtcacgt cggtatagcc
     8041 ggggcacaac atatagtggt ttcagacgaa cggcggtttc agacaagcgc acatccggtt
     8101 gttgcttcca ggcgacaaaa atattcgagg gagaaaaaat gcaacttgcc accaacctga
     8161 cggcggccac tccggtaggc aattactcca ctgaagatgg cgcggccgac cgggatccac
     8221 agcgctacgc ccagtacaaa gtcatccgcc gcaacggcgc cgtcgtcggc ttcgagccgg
     8281 gcaagatttc ggttgccgtg accaaggctt tcctggccgt caacggcggt cagggtgctg
     8341 cttcggcccg tgttcgcgag ctggtcgaga agcttaccga gactgtggtg aacgcgctgg
     8401 tccgccgcca gccgaacggc ggcacctttc acatcgaaga catccaggat caggtcgaac
     8461 tctctctgat gcggtcgggc gagcacgaag tggcccgtgc ctatgtgctg tatcgggaag
     8521 cgcgcgcgcg cgagcgcgcc aggaaccgcg agacgcaggc agcggccacg gcgcacgcga
     8581 tcagcgtcat cgaacacggc cagacccatc cgctgaacat ggagcggctg accggtctga
     8641 tcaaggattc ctgcgaaggc ctcggccgcg cggtcggtcc cgctgtgatc ctgcagatga
     8701 cgctcaagga cctgtacgac ggcgtgccga tggacgaggt gcgcaaatgc gccatccttg
     8761 ccgcccggtc gctgatcgag aaagacccgg catacagcta cgtcaccgcg cgcctcttac
     8821 ttaatagtgt ccgctacgag gtcctgggcg aagaagtcac ccaggggcaa atgcattccc
     8881 gctacgccga atacttcccc gggttcatca aacgggggat cgacgcggga ttgctggacg
     8941 agcggctcgg gcgtttcgac ctgcgctcgc tgggcgcggc gctggatgcc aaccacgatt
     9001 tgaaattcgg ttacttgggc ctgcaaacgc tctatgaccg ctacttcctg cacgtgcacg
     9061 gtcaacgcat tgagcttccc caggcctttt ttatgcgcgt ggcgatgggt cttgccatca
     9121 acgaagtcga gcgcgaggcc cgcgccatcg agttctacaa cgtactgtcg cgcttcgact
     9181 tcatgtcctc gacgcctacg ctgttcaact ccggcacgca ccgctcgcag ctttccagct
     9241 gctacctgac cacggtatcg gacaacctgg aaggcattta cgacgccata aaagaaaacg
     9301 cgttgctttc gaagtttgcc ggcggcctgg gaaacgactg gactccggtt cgggcgctgg
     9361 gctcccatat caagggaacc aacggcaagt cgcagggagt cgtgcctttc ctcaaggttg
     9421 taaacgacac cgcagtcgcg gtaaaccagg gtggcaagcg caaaggggcg gtgtgctcgt
     9481 acctggaaag ctggcacctg gatatcgagg agttcctcga gctgcgcaag aacaccggag
     9541 atgaccgccg ccgcacccac gacatgaaca ccgccaactg gattccggac ctgttcatga
     9601 agcgggtcat ggaagctggc gagtggacgc tgttctcgcc cgcagatgtg cccgacctgc
     9661 acgacaaggt cggcaaagaa ttcgagaagg cctataccga atacgagcgc aagacccaga
     9721 ccggcgaact caagctcttc aagcgcatcc cggttttgac gctgtggcgc aagatgctga
     9781 ccatgctgtt cgagaccggg catccctgga tcaccttcaa ggatccctgc aacatccgca
     9841 gcccacaaag ccacgtcggc atggtgcaca gttccaacct ttgcaccgaa atcacgctca
     9901 acacttcgga tagcgagatc gcggtctgca acctgggttc gatcaacctg gtcgcgcaca
     9961 tgaaggaggg caagctcgat cacgacaagc tcaagcagac ggtatccacc gcgatgcgca
    10021 tgctcgacaa cgtgatcgat gtcaactact acgcggtcaa caaggcgcgt aacgccaact
    10081 tcaagcatcg cccggtcggt ctgggcatca tgggttttca ggactgcctg ctcgagatgc
    10141 gcgtgcctta cgcttcgaag gaagcgctgg acttcgccga ccgttcgatg gaggcggtgg
    10201 ccttctacgc ctattgggcc tcaaccgagc tggccgagga gcgcgggcgc tattccactt
    10261 atgaagggtc gctgtggtcc aggggcatcc tgccgcagga ctcgctcaag ttactggctg
    10321 aggagcgcgg cggctacgtc gaggtggata gctcttcgcg tatgagctgg gagccgttgc
    10381 gcgcgcgtat caagcagttc ggcatgcgta attccaactg cctggcgatc gcgccgaccg
    10441 caaccatcgc caatatcgtc ggcgtatcgg cctcgatcga accgacctat cagaacttgt
    10501 acgtgaagtc gaacctgtcc ggcgagttca cggtcaccag cgagtacctg gtccgggacc
    10561 tcaagaagct caacatatgg gacgaggtca tgatcgccga cctcaagtac tttgacggca
    10621 gcctggccaa gatcgaccgc gttccccaag aggtccgcaa cctctacgcc accgcgttcg
    10681 aagtggactc ggcatggatc gtcgaggcgg gcgcgcgccg gcagaagtgg atcgaccagg
    10741 cgcaatcgct caatatctat atggcgggcg cctccggcaa gaagctggac gacatctaca
    10801 agctggcctg gaagcggggg ctcaagacca cttattacct gcgcacgctg ggtgcgacct
    10861 cggcggaaaa atccaccgcc cgtggcggcc agttgaacgc cgtacccacg gatggtggcg
    10921 tcatgggtgc gggttcgacc gccgcgtcgg cggacgagga aacaaagttt tgttccatag
    10981 ataaccccga atgcgaggcc tgccagtgag gccggccttc cgcctgcggt tcgccgcacg
    11041 ccgccgtccg acgttctggt tgctgcgcag ccgcaacgaa atcacgcggt tcgcggtggc
    11101 gacgaagccg ccattcagag gccgggacta tttttgagcg atgagccgat cggaattcgt
    11161 cttcatcgcg atcgaaggat aaaaaggggt cgcattgcgg ccgaatattt aaagaagtat
    11221 ttgacattcc accgataaca tttataaagg aaccaagcat gctgcaattc gagaatcaca
    11281 ttccgagcac cggcggcgct ggtttgcagc gcgtcgttgt caacatcccg gcgggcaatg
    11341 ctccgccggc acgcgttgcc gaatccggtc cgtccgcgga cgttgcgttc cgtcgcgtgc
    11401 gtgttgaaga caaacttgtc atcaacggca aggccgacgt caaccagctc gtcccgttca
    11461 agtacaagtg ggcctgggac aaatatctgt ccggctgcgc caaccactgg atgccccagg
    11521 aaatcaacat gagccgggac atcgccacct ggaaggatcc gaacggcctg accgaagacg
    11581 aacgcctggt ggtcaagcgc aacctcggtt tcttcgtgac cgccgatagc ctggcggcca
    11641 ataacatcgt gctcggcacc taccggcaca tcaccgcccc ggagtgccgg cagtacctgc
    11701 tgcgccaggc tttcgaagag gcgatccaca cccacgccta tcagtacatc gtcgagagcc
    11761 tgggtctgga cgagggcgag atcttcaacg cctaccacga agtctccagc attcgggaca
    11821 aggacgagtt cctgatcccg ttcatcgaaa cgttgaccga tccggaattc aagaccggca
    11881 ccccggcggc cgaccagcag ctcctgaaga gcttgatcgt gttcgcctgc atcatggagg
    11941 ggttgttctt ctacgtcggc tttgcccaga tcctggcgct ggggcgccag aacaagatga
    12001 ccggtgccgc cgaacaatat cagtacatcc tgcgcgacga gtccatgcac tgcaatttcg
    12061 gcatcgacct gatcaaccag atcaagatgg agaatccaaa cctgtggacc ccggagttcc
    12121 gcgaggaaat ccgcggtctg atccagaggg gcgtggaact cgagtaccgc tacgccgagg
    12181 acaccatgcc acgcggcgtt ctgggcctga acgcgccgat gttcaaggag tacctgcgct
    12241 tcgtggccaa ccgccgcgcg caacagatcg gcctcgatcc gctgttcccg ggtgcgacca
    12301 atccgttccc gtggatgtcg gagatggtcg atctcaagaa agaaaagaac ttctttgaga
    12361 cccgtgtgat cgagtatcag accggcgggg ctctgaactg ggattgatgc atgggccgga
    12421 aagttttctg tgacacccgt gctgccgccg gtcgattcaa cggcccggca gcgcacttgt
    12481 tagggctgaa tgggtaa
//
LOCUS       DASQOP010000053        10081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1229018, whole genome shotgun sequence.
ACCESSION   DASQOP010000053 DASQOP010000000
VERSION     DASQOP010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10081
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229018"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VE030_01980"
     CDS             <1..271
                     /locus_tag="VE030_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006407013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HZF22214.1"
                     /translation="VPWKYGFKSAKSIVRIRFSEQQPKTAWEMAAPHEYGFYSNVNPK
                     VDHPRWSQAKERRIGEFRKRETLIFNGYADQVASLYSGMDLKKNF"
     gene            451..1092
                     /locus_tag="VE030_01985"
     CDS             451..1092
                     /locus_tag="VE030_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HZF22215.1"
                     /translation="MKQPKPEYIPWIKAAVFVACLVPLARLAWLGFHNRLGANPIEYI
                     THSTGWWTLALLLITLSVTPLRRLLNLPWLLRLRRTIGLFAFFYVSLHFTTYIWLDQF
                     FDWTEIVKDIGKRPFITVGFSAFVLLIPLAATSTNAMVKRLGAGRWRLLHRLVYGIAI
                     LGVVHFWWLVKKDITEPFTFAWLLALLLIIRLLYLLKQQRSASPPSAARSQVV"
     gene            1102..2226
                     /gene="ald"
                     /locus_tag="VE030_01990"
     CDS             1102..2226
                     /gene="ald"
                     /locus_tag="VE030_01990"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HZF22216.1"
                     /translation="MLVGVPREIKDHEYRVGLTPAGVHALCERGHEACVEIGAGARIG
                     FPDGDYLSAGARIVASAKEVFAADMVVKVKELQPAEFGLPRRGQILFAYLHLAPDPEL
                     LEALLACGVSGIAYETVTDARGRLPLLAPMSRIAGRLSVQVGAWALQMANGGSGVLLG
                     AVPGVVPGRVVVIGAGASGANAVQIAAGMGADVTVLDVDLERLAHLDEIYRGRVKTCY
                     SEALAIAEQIAQADLVIGALLVRGKLAPRLISRRLLRSMRPGSVLVDISIDQGGIAET
                     SRPTSHSQPLFVEEGVVHYCVPNMPAACARTATLGLTQATFPYVLRLADRGLDALSED
                     AGLANGLQVHAGQVTHRGLAEDVKRPFRSYAEIAAGMRRA"
     gene            complement(2410..3306)
                     /locus_tag="VE030_01995"
     CDS             complement(2410..3306)
                     /locus_tag="VE030_01995"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZF22217.1"
                     /translation="MRWSFIVLAGAVTVVSTASILIRLAQAEGASSLTIAAARLGLAA
                     AILAPFAWLKAGREIMRLGRRELGMCLLSGLFLAMHFWTWISSLEYTSVASSAALVTT
                     NPLWVGLASTVLLRERPAPAALAGTALIVAGSVLIFTADSGGNERTATYPLLGNALAL
                     AGAISASGYLLVGRALRARISLTAYVWLAYTVAALLLGTAVAASGSSLAGLSGPAWGF
                     LALLAIGPQLVGHTAFNWAVRRLTVIFVAIAILGEPVGAAILAWFLFDEGFTALQLAG
                     FVLLLAGIFVAARGEKSREIQN"
     gene            complement(3580..4014)
                     /locus_tag="VE030_02000"
     CDS             complement(3580..4014)
                     /locus_tag="VE030_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HZF22218.1"
                     /translation="MIVFNLSCEKGHRFEGWFGSADHFAEQVKGGGIACPLCGSPDIA
                     RQPSAPYVSTRSSEPSQEPKSVMVANAARSLHKKFVEYVLQNTEDVGKQFPDEARRIF
                     YKEVAERAIRGTASMKEVTELKEEGIDVLAVSGIPDPPETLH"
     gene            complement(4139..5380)
                     /gene="lysA"
                     /locus_tag="VE030_02005"
     CDS             complement(4139..5380)
                     /gene="lysA"
                     /locus_tag="VE030_02005"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HZF22219.1"
                     /translation="MSFFSQRNGTLHAEDVPLARIAEEFGTPCYVYSRAALTDAFQRY
                     DRAFGARAHLVCYAVKANSNLAILNLFARLGSGFDVVSGGELARVIAAGGEPGKTVFS
                     GVGKTEREMREALAAGILGFNVESESELLRLERVAGKMGRHAPVSLRVNPDVDVRTHP
                     YISTGLKQSKFGIAFRDAIRLYRLAHSSRHLKVVGIDCHIGSQLVEVEPVADAVRKVL
                     KLADSLASEGIEMEHIDLGGGLGIRYADEVPPTQDAWVKALLDAVGDRPQSLLVEPGR
                     SLVGNAGLLLTRVEYLKHGEAKNFAVVDAAMNDLIRPALYDAWHEVRAVKDSGAAAVY
                     DVVGPVCESGDFLAKERQLAIDEGSLLAIMSAGAYGMSLSSNYNARPRAAEVVVDGGR
                     FNLIREREQVEDLFAGERLLP"
     gene            complement(5842..6576)
                     /locus_tag="VE030_02010"
     CDS             complement(5842..6576)
                     /locus_tag="VE030_02010"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HZF22220.1"
                     /translation="MFSVAAVSEPKTIRLGNRDVPYLLKRSQQRRSIVLTVDERGLIV
                     SAPWRSTERRIAGIIREAEDWVLRKLAVWSAYPSRNQSWTDGDTIKYLGRDLRLHLRE
                     DRTATLATLGDADCLQVALPEHGGSEAVRAAVLKWYRRHATSNFSERIGVYARLYGLA
                     IPRLFLSGAQTRWGSCNVKREVRLNWRLIQAPQPTIDYVVAHELAHLVEMNHSRRFWN
                     LVAAVCPHYRQARAELDHMGRYYMDI"
     gene            complement(6579..7304)
                     /locus_tag="VE030_02015"
     CDS             complement(6579..7304)
                     /locus_tag="VE030_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZF22221.1"
                     /translation="MSIVAAFLRSAVFEIVRFALTVVFAVISLLTFAFEPLTRYRIIT
                     TWSRLVVALARAVCGVEFRVIGRENLPAKPSIVLSKHQSAWETLAYQKILPPQVWVLK
                     RELLQVPFFGWGLAMMSPIAIDRGSATRSLRQTLEQGKDRLAKGWWIVIFPEGTRIAP
                     GRRGRYHLGGAWLACHTGAPVVPIAHNAGTLWRKNAFIKHPGTITVSIGQAIHPQGMT
                     ADTLNRKVEDWIETEVARLGNAR"
     gene            complement(7301..7834)
                     /gene="gmhB"
                     /locus_tag="VE030_02020"
     CDS             complement(7301..7834)
                     /gene="gmhB"
                     /locus_tag="VE030_02020"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HZF22222.1"
                     /translation="MKLVILDRDGVINQDSDQFIKNPEEWKPIPGSLEAIARLNHTGY
                     RVVVASNQSGIGRGLFDMGALNAINDKMYRALAQVGGRIDALFYCPHAAEADCDCRKP
                     RPGMFLDIAQRFNVDLAGVPSVGDSLRDLQAAAAARAQPMLVLTGKGAKTKGAGGLPD
                     NTMLFANLAEAVRHIVS"
     gene            complement(7896..10031)
                     /gene="glyS"
                     /locus_tag="VE030_02025"
     CDS             complement(7896..10031)
                     /gene="glyS"
                     /locus_tag="VE030_02025"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HZF22223.1"
                     /translation="MSSNLLVELVTEELPPKTLRRLGDAFAGAIFDGLKGSGVLDATS
                     RFKAFATPRRLAVYIWAVLAKAGDRPVEQKLMPVAVARNSDGAWSDALRRKLASMGRE
                     RLADVPANSKVGGESLVIKPDGKAEAVFLRSIAAGQPLERMLSGAIEQAIAKLPIAKV
                     MSYQLADGATTVKFVRPAHALVALHGDKVLNVSALGLAAGRITHGHRFQGEKNIALVR
                     ADEYESRLEHEGRVIADFEKRRDDVSRQLAENAAALKASLGPEQDIAPLLDEVTGLVE
                     LPTVYVGEFEPEFLDVPQECLILTMRQNQKYFPLFDTAGKLTNKFLIVSNMRLADPQN
                     IVAGNQRVIRPRLADARFFFETDKKTRLADRVPQLASIVYHNRLGSQLDRVGRVRALA
                     RAIAPMIGADPLLADRAALLAKADLVASMVGEFPALQGIMGRYYALADAEDAHVAGAI
                     EEHYRPRFAGDALPGGEIATALALADKLEMLAGLFGIGQQPTGDKDPFALRRHALGVI
                     RMLIEGKLPVSLHDLIDAAFSVFPRDMIGDTRAPLQDFIFERLRSYLRDAGYTANEIE
                     AVLCMRPAQLHQVPLQLAAVRAFASLPEAESLAAANKRVVNILKQAEAKGESFNGAQA
                     GILTETAERELFDALATATRNAIPLLQKGDYTGYLKAFAVLKSPVDAFFDSVMVMVDD
                     AAVRRNRIALLADLRQEMNRIADISKLAA"
BASE COUNT         1914 a         3095 c         3134 g         1938 t
ORIGIN      
        1 ggtgccgtgg aagtacggct tcaagagcgc gaagtcgatc gtgcgcatcc gcttttccga
       61 acagcaaccg aagaccgcct gggaaatggc ggccccgcac gagtacggat tctattccaa
      121 cgtaaacccc aaggtcgatc acccgcgctg gagccaggcg aaagagcgcc gcatcggcga
      181 gttccgcaag cgcgaaacgc tgatattcaa cggctacgcc gaccaggtcg cttcgctcta
      241 ctccggcatg gatctgaaga agaacttctg aaaggaatct tgaaccacgg aggacacgga
      301 ggacacggag gaagggcaaa ggcagaacaa tttccgagag taaaaatctg gtatcggtgg
      361 tcactcttgc accgggtctg cttttcagtc ccacatgttt tttcttgttt ctggttttcc
      421 tccgtgtcct ccgtggttca tatctgaatt gtgaaacagc ccaagcctga atacattccg
      481 tggatcaagg cagcggtgtt cgtcgcttgt ttggtcccgc tcgcgcgact ggcctggctg
      541 ggattccata acaggctcgg cgccaacccg atcgagtaca tcacccattc caccggctgg
      601 tggacgctcg cgctcctgct gatcacgctg tcggttacgc ccttgcgccg gctgctcaac
      661 ctgccgtggc tgttgaggct gcggcggacg atcggattgt tcgcattttt ctacgtcagc
      721 ctgcacttca ccacttacat ctggctcgac cagttcttcg actggacgga gatcgtcaag
      781 gatatcggca agcggccctt catcaccgtg ggtttcagcg cgttcgtgct cctgatcccg
      841 cttgccgcga cttccaccaa cgcgatggtg aaacgtctcg gtgctggacg ctggcggctg
      901 ctgcatcgcc tggtgtacgg gatcgccata ctcggcgtag tgcatttctg gtggctggtg
      961 aagaaagaca tcaccgaacc attcaccttt gcctggctgc tcgcgcttct cctgatcatc
     1021 cggttgctgt acctgctgaa gcagcagcgt tcggcatccc ctccttccgc ggcacgctcc
     1081 caggtagtct gagggcttcg catgctggtc ggcgtgccca gagaaatcaa ggatcacgag
     1141 taccgcgtag ggctcacgcc ggcgggtgtc catgcgctct gtgagcgcgg ccacgaggcc
     1201 tgcgtcgaaa tcggtgccgg cgcacgcatc ggttttcccg atggcgacta cctatcggct
     1261 ggcgcacgca tcgttgcctc ggcgaaggaa gtctttgccg cggacatggt ggtgaaagtc
     1321 aaggagttgc agccggcgga gttcgggctg ccgcgccgcg ggcagatcct gtttgcctat
     1381 ctgcacctgg caccggaccc ggagttgctg gaggcattgc tggcttgcgg tgtcagcgga
     1441 atcgcttatg agacggtgac cgacgcgcgg ggccggctgc cgttgctggc accgatgtcg
     1501 cggatcgccg gcaggctgtc ggtacaggtg ggtgcctggg cgttgcagat ggccaatggc
     1561 ggcagcggcg tgctgctcgg cgcagtgccc ggcgttgtgc cgggaagggt ggtggtgatc
     1621 ggggccggcg cctccggcgc gaacgcagta cagatcgcgg ccggcatggg cgccgacgtc
     1681 acggtgctcg atgtcgacct tgagcgcctc gcgcatctcg acgagattta ccgcggccgg
     1741 gtgaaaacct gttactcgga agcactggcg attgcagagc agattgcgca ggccgacctg
     1801 gtgatcggag cgcttctcgt gcgcggcaag cttgccccga ggttgatctc acgcaggctt
     1861 ctgcgaagca tgcggccggg ttcggtgctg gtggacattt cgatcgatca ggggggcatt
     1921 gccgagacct cgcgcccgac gtcgcactcg cagccactgt tcgtggaaga aggcgtggtg
     1981 cactactgcg tgcccaacat gcccgcggcc tgcgcgcgta ccgcgacgct ggggctgacg
     2041 caggcgactt tcccctatgt gctgaggctt gccgaccgcg gactcgacgc gctatctgag
     2101 gatgcggggc tggcgaacgg cttgcaggtc cacgccgggc aggttaccca ccgcggcctg
     2161 gctgaagacg tgaagcggcc gttccgcagc tatgccgaga tcgccgctgg tatgcgtcgg
     2221 gcctgaggca aactgcaaaa tcgaaaatct caccacgaag acacgaagaa aagccaaagt
     2281 ctggtcgaat tctgcgaact gcaatttagg ccgtgcaacc ttacccggta ttcatatctt
     2341 tccctttggc acttctccgt atttcttcgt gtcttcgtgg taagaatttg attttttgtg
     2401 gcaaagcttt cagttctgga tctcgcggga tttttccccg cgagcggcga cgaagatgcc
     2461 ggcgagcaac aacacgaaac cggccagctg cagcgcggtg aatccttcat cgaagaggaa
     2521 ccaggcgaga atcgctgcgc ccaccggttc gcccaggatc gcgatcgcaa cgaagatgac
     2581 agtgagccgt ctcacggccc agttgaaagc cgtgtgtccg acgagctgcg ggccgatcgc
     2641 aagcaatgcc aagaatcccc atgccggtcc ggacaagccc gccagcgagc tgccgctagc
     2701 cgccaccgcg gtgcccagca gtagcgccgc caccgtgtag gccagccaga catacgcggt
     2761 gagtgatata cgcgcgcgca gcgcgcgtcc caccagcaga tagcccgagg cggagatcgc
     2821 tccggccagc gccagcgcat tgcccaagag cggatatgtc gccgttcttt cgttcccgcc
     2881 ggaatcggcg gtaaagatca gcacgctgcc ggcgacgata agcgcggtgc ccgcgagtgc
     2941 agcaggcgcg ggacgttcgc gcaacagcac tgtagaagcc aacccgaccc agagcggatt
     3001 ggtggtgacc agcgccgcac tgcttgccac cgacgtgtac tccagcgagg aaatccacgt
     3061 ccagaagtgc atggccagaa acagcccgga cagcaggcac atgcccagtt cgcgtcggcc
     3121 cagccgcatg atttcccgtc ccgctttcag ccaggcgaaa ggcgccagaa tcgcggcggc
     3181 gagccccagc cgcgccgcgg cgatcgtgag ggaagatgca ccttcggctt gcgccagccg
     3241 gatcaggatc gaggcggtcg agaccacagt gaccgcaccg gcgaggacga tgaacgacca
     3301 cctcatcccc ctctccctgt ccctcttccg ctaaggggag agagaaccaa aaaactttcc
     3361 atgcaggtcc ctttgagcga agcctcggta acagctgttg ccatcacgta ccctcaactg
     3421 ataaaggatt caaatctaaa ctattcgtca tgtgtccgct ttcgcaaaca cggagaatga
     3481 aatgaaaacg ggcgaccatg agatcgcccg ttttgtccta gtgcctcaaa gttccctctc
     3541 ccctcgcggg agaggggcag ggagaggggg acaggtcgtt cagtggagtg tctctggcgg
     3601 atccggaata ccggaaacgg cgagaacgtc gattccctct tccttcagtt cggtcacttc
     3661 tttcatggat gcagtcccgc gaattgcgcg ctcggcaact tccttgtaga aaatccgccg
     3721 tgcttcatcc gggaactgct tgccgacatc ctcggtgttc tggagcacgt attcgacgaa
     3781 cttcttgtgc aacgaccggg cggcattagc gaccatgacc gattttggtt cttgcgacgg
     3841 ttcgctgctg cgtgtgctga cgtagggcgc tgaaggctgc ctggcgatgt caggcgaacc
     3901 gcacagcggg caggcaatgc ctccgccctt cacttgttcg gcgaagtggt cggccgagcc
     3961 aaaccaccct tcaaaacggt ggcctttttc gcaggaaaga ttgaaaacga tcatagttat
     4021 gttggtcgcc aggattgcga ggcggcgcaa agtttaccat ctgcggccgg tgaatttgcc
     4081 ggcctgcaag gagtagaacc ggccgcgcag cgttagttcc gcgcctagcg ccaacggatc
     4141 agggcagcag ccgttctccc gcgaacaggt cttcgacttg ctcgcgttcg cgtatcaggt
     4201 tgaagcgccc gccgtcaacc accacttctg cggcgcgtgg gcgggcgttg tagttcgaac
     4261 tcaggctcat gccgtaagcg cctgcagaca tgatcgccag aaggctaccc tcgtcgatcg
     4321 cgagctgccg ctccttggcg agaaagtcgc cgctttcgca taccggaccc accacatcgt
     4381 agaccgcagc agcaccggag tccttgactg cacgcacttc gtgccaggcg tcgtacagcg
     4441 ccggacgaat caggtcgttc atcgcggcgt ccaccaccgc gaaattcttc gcctcgccat
     4501 gcttcaggta ctcgacccgg gtcagcaaca ggcccgcatt gccgaccagc gaacggcccg
     4561 gctcgaccaa caacgattgc ggccggtccc cgaccgcatc cagcaatgcc ttcacccagg
     4621 cgtcttgcgt gggcgggact tcatctgcgt agcgaatgcc gagacctccg cccaggtcga
     4681 tatgttccat ctcgatacct tcggacgcca gactatcggc gagcttcagc accttgcgca
     4741 cggcatctgc gaccggttcc acctcgacca gttgcgagcc aatgtggcag tcgatgccga
     4801 caaccttcag gtgcctggac gaatgtgcca gacgatatag gcgtatcgcg tcccggaacg
     4861 cgattccaaa cttgctttgc ttcaggccgg tggaaatata ggggtgtgtc ctgacgtcga
     4921 catccggatt cacccgcagg ctgaccggcg cgtgccggcc catcttgccc gcgacgcgtt
     4981 cgaggcgcag gagttcggat tcggactcca cgttgaagcc aaggatgccg gcagccagcg
     5041 cctcgcgcat ctcgcgctcg gtcttgccca cgccggagaa cactgtcttg ccaggttctc
     5101 cgccggcggc gatgacccgt gccagttcgc cgccggagac gacgtcgaag ccgctgccca
     5161 gacgggcgaa aagattaagg atcgccagat tggaattggc cttgaccgca tagcagacca
     5221 ggtgcgcacg cgcgccaaag gcgcgatcgt aacgctggaa ggcgtcggtc aacgccgcgc
     5281 gcgagtagac gtagcacggc gtgccgaatt cctcggcaat gcgcgcaagc ggcacgtctt
     5341 cagcgtgcag cgtgccgttg cgctgggaaa aaaaactcac ttctcgatag ttccttcttc
     5401 ggtttttctg ggcttctgct cttccttcgg cagatacagg gggcccttct ggccgcaggc
     5461 ggagatcagg aacgcgagca ggactaggac gcaaaggcgg cgcatggaag actttctagg
     5521 aagcagctga gtttagcaca gcgtcttgcc gtaaccagcc atccgcaaga cgaaaagtca
     5581 aagtctcacc gcgaaggctg acaacaacaa acctgaacgc tcaccacgaa ggcacgaaga
     5641 cacgaagaaa gactttcaaa gtgaacgaca aaaatgtctt gttggccagc acgcacggcc
     5701 cgaatacgcc agtcatctga gaacatcatc acctcattca aagttcccgg cggccaaaaa
     5761 agctaccttt accaattttc tttttcttcg ctcttcttcg tgtcttcgtg ccttcgtggt
     5821 gagattcttg atttccagaa atcaaatatc catgtaatag cggcccatgt ggtcgagctc
     5881 ggcgcgcgct tgcctgtaat gcgggcacac ggctgcgacc aggttccaga atcgtcgcga
     5941 gtggttcatc tcgacaagat gggcaagttc gtgcgcgacg acgtaatcga tcgtcggttg
     6001 cggcgcctgg atcagccgcc agttgagcct gacttcgcgt ttgacgttgc aactgcccca
     6061 gcgcgtctgt gcgccggaaa gaaacagccg cgggattgcc aggccgtaca gacgggcgta
     6121 tacgccgatg cgctcggaga aattcgacgt cgcgtggcgg cgataccatt tcaggaccgc
     6181 ggctctgacc gcctcggaac cgccgtgttc gggaagcgcg acctgcaggc agtccgcgtc
     6241 gcccagggtc gccagtgtcg cggtccggtc ttccctgaga tgcagccgca gatcgcggcc
     6301 aagatatttt atggtgtcgc catcggtcca cgactggttg cgggagggat acgcgctcca
     6361 gacggcaagt ttcctgagga cccagtcctc cgcttcgcga atgatgccgg cgatgcggcg
     6421 ctccgtgctg cgccaggggg cgctcacgat cagcccgcgc tcgtccacgg tcagcacgat
     6481 gctgcgccgt tgctggctgc gtttgagaag gtaggggacg tcgcggttgc ccagccggat
     6541 ggttttcggc tcggagaccg cggcgaccga gaacaaatct agcgagcgtt cccgaggcgg
     6601 gcgacttcgg tttctatcca gtcttcgacc ttccggttga gcgtgtcggc ggtcatgccc
     6661 tgcggatgaa tggcttggcc gatgctcacg gtgatggtac ccgggtgttt gatgaaggcg
     6721 ttcttgcgcc acaaggtccc ggcattatgc gcgatcggca ccaccggcgc gccggtatgg
     6781 cacgccagcc aggcgccgcc gagatgatat cgtccacggc ggccgggggc gatgcgggtg
     6841 ccttccggaa atatcacgat ccaccagcct ttggcgaggc ggtccttgcc ctgctcgaga
     6901 gtctgcctca gggagcgggt ggccgagccg cggtcgatgg cgatcgggct catcatcgcc
     6961 agtccccagc cgaagaatgg cacctgcagc aactctcttt tcaggaccca gacctgcggc
     7021 ggaaggattt tctggtaggc gagcgtttcc caagccgatt ggtgtttcga cagcacgatg
     7081 cttggcttcg cgggcaggtt ttcacggcca atcacgcgga attcaacgcc gcagacggcc
     7141 cgcgccagcg cgaccaccaa gcgcgaccag gtggtgatga tgcgatagcg ggtcagcggt
     7201 tcgaacgcaa aggtgagcag cgaaatgacg gcgaagacta ccgtgagtgc gaaccgcaca
     7261 atctcgaaga cggccgaacg caggaaggcc gcaacgatgc tcatgaaacg atatgccgca
     7321 ccgcttcggc aaggttggcg aacagcatcg tgttatccgg cagaccaccc gcacctttgg
     7381 tcttggcgcc cttgccggtg agtaccagca tcggctgggc gcgggcggcg gccgcggcct
     7441 gcagatcacg cagcgaatct cccacgctcg gcacgccagc caggtccacg ttgaaccgct
     7501 gggcgatgtc gagaaacatc cccggcctgg gcttacggca gtcgcagtcc gcttccgccg
     7561 cgtgcgggca gtagaacaat gcgtcgatgc gcccgccgac ctgggccagc gcacggtaca
     7621 tcttgtcgtt gatcgcgttc agcgccccca tgtcgaacag gccacgaccg atgccggact
     7681 ggttggaggc cacgaccacg cggtagccgg tatggttcag ccgcgcgatt gcctccaggc
     7741 ttcccgggat cggcttccac tcctcggggt tcttgatgaa ctgatcgctg tcctggttga
     7801 tgacgccgtc gcgatcgagg atgactaact tcatgggact gaggggcgag ggacgagggg
     7861 cgagggtcga ggcaaaccgt gcgcaagcat gtcgatcatg cggccagctt ggaaatgtcg
     7921 gcgatgcgat tcatttcctg gcgcaaatcc gccagcagcg cgatgcgatt ccggcgtacc
     7981 gcagcgtcat ccaccattac catgaccgaa tcgaaaaatg cgtcgacggg tgatttcagc
     8041 acggcgaatg ctttcaggta accggtgtag tcgccctttt gcagcagagg gatcgcgttc
     8101 ctggtggccg tcgcgagcgc atcgaatagt tcgcgttcgg cggtctcggt caatatcccg
     8161 gcttgggcgc cattgaagga ctctcctttg gcctcggcct gcttgaggat attgaccaca
     8221 cgcttgttcg cggcggcgag actctctgcc tccggcaggc tggcgaaagc gcgcacagcg
     8281 gcaagttgca ggggtacctg atgaagctgg gccggccgca tgcacagcac cgcttcaatc
     8341 tcgttcgcgg tatagcccgc atcgcgcagg taactgcgca ggcgttcgaa aatgaaatcc
     8401 tgcagggggg cgcgggtgtc gccgatcatg tcgcggggaa aaaccgagaa tgccgcgtcg
     8461 atcagatcgt gcaacgatac cggcagtttg ccctcgatga gcatgcggat cacccccaat
     8521 gcatggcgac gaagcgcgaa cggatccttg tctccggtcg gctgctggcc gattccgaac
     8581 aggccggcga gcatttccag cttgtcggcc agcgcgagcg cagttgcaat ctcgccgccg
     8641 ggcaacgcgt cgcctgcgaa tcgcggccga taatgctcct cgattgcgcc ggccacgtgc
     8701 gcgtcctctg catcggcgag cgcgtaatac cggcccatga tgccctgaag cgcggggaac
     8761 tcgccgacca tgctggcgac cagatcggcc ttggcgagca gcgccgcacg gtcggcaagc
     8821 agcggatcgg cgccgatcat cggtgcgatc gccctggcca gcgcacgaac gcgtccgacg
     8881 cgatcgagct ggctgccgag cctgttgtgg taaacgatgg aagcgagctg gggcacgcgg
     8941 tcggcgagcc ttgtcttctt gtcggtctcg aagaaaaagc gcgcgtcggc caaccgcggg
     9001 cggatcacgc gctgattgcc cgcaacgatg ttctgcggat cggcaaggcg catgtttgaa
     9061 acgatcagga acttgttggt gagcttgccg gccgtgtcga acagcgggaa atacttctga
     9121 ttctgccgca tggtcaggat gaggcattcc tgcggtacat cgagaaactc gggctcgaat
     9181 tccccgacgt acacggtcgg caactcgacc agtcccgtga cttcgtcgag caacggcgcg
     9241 atgtcctgtt ccggtccgag cgaagccttg agcgcggcag cgttctccgc gagctggcgg
     9301 ctgacgtcgt ccctgcgctt ttcgaaatcg gcgattacgc ggccttcatg ctcgagccgg
     9361 gattcatatt cgtcggcacg caccagggca atgttcttct cgccctggaa gcgatggcca
     9421 tgagtgatgc ggccggccgc caggccgagc gcggaaacgt tgagtacctt gtcgccgtgc
     9481 agcgcgacca gcgcatgggc ggggcggacg aatttgacgg tcgtggcgcc gtcggcgagc
     9541 tggtagctca tcaccttcgc aatgggcagc ttggcgatgg cttgctcgat cgctccggat
     9601 agcatgcgtt cgagcggttg acccgctgcg atgctacgca ggaatacggc ttcggccttg
     9661 ccatccggct tgattaccag cgactcgccg ccaactttgg aatttgccgg aacatcggca
     9721 agccgttcgc ggcccatgct cgcgagcttc ctgcgcagcg cgtcgctcca ggcgccgtct
     9781 gaattgcgcg cgacggcgac cggcatgagc ttttgctcga ccggacggtc gccagccttg
     9841 gccagcaccg cccagatgta aaccgccaag cgccgcggcg tggcgaatgc cttgaacctg
     9901 gacgtggcat ccagaacccc gctgcctttc agtccatcga atattgcacc ggcaaaggca
     9961 tcgcccagcc gtctgagagt ttttggaggt agttcctcgg tgacgagttc gaccagcaga
    10021 ttcgaactca tgcgggttgt cttgccagca gagtcgcctt catagcctgg aggtcgagac
    10081 c
//
LOCUS       DASQOP010000054         5964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_220921, whole genome shotgun sequence.
ACCESSION   DASQOP010000054 DASQOP010000000
VERSION     DASQOP010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5964
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220921"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="VE030_02030"
     CDS             <1..1057
                     /locus_tag="VE030_02030"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HZF22224.1"
                     /translation="QGQDTIETTYFASANRGKKSVTVDITRGEGQELVRKLAAKCDVF
                     IENFKTGTLSRYGLAYQQLRQARQDMVYCSITGFGQTGPYAVRPGYDPIAQALSGLMS
                     VTGESEDMPGTVPQRVGVAVIDLMTAHYAVIAVVSALYHRQATDRGQYIDMSLLDVGV
                     ASMANIASAYLGAGVIAKRNGGVHPSVVPSQVFLCKDGYVIVAAGNDGQFAKLCEVGS
                     KGDLVRDARFSTNSARVKNRDALRPLLEAMFLKHPVTWWNAKLAAAGVPCAPINDIAQ
                     VFEDPQVQHRGLRVEMPEPAAGKAAFVASPLRFSESPVEYRLPPPLLGEHNQEVFEDL
                     LGMSPEEIARLRASGVI"
     gene            1080..1370
                     /locus_tag="VE030_02035"
     CDS             1080..1370
                     /locus_tag="VE030_02035"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZF22225.1"
                     /translation="MTIDPAAIPVVHNTAAKRFEITIGGKTAYSKYLLAGDKIIIEHT
                     EVPEALEGRGLASRMVSTALDYARAKNLKVLPICPFTKSFIGRHSEYQDLLA"
     gene            1427..2833
                     /locus_tag="VE030_02040"
     CDS             1427..2833
                     /locus_tag="VE030_02040"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF004815.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZF22226.1"
                     /translation="MADEDLCFTPATELTRMIRDRLVSPVEVMKVTVERAQKLNTRLN
                     AICTPTYDRAMAAARDAEAALMRGAALGPLHGIPTSIKDLAFTKGVRTMAGSHIFSNR
                     VPDFDHVHVARLRAAGGISIGKTTTSEFGWTGVSRSPLTGITHNPWKHGMNAGASSAG
                     AAVCAAAGIGPIHQGSDGAGSIRMPASFCGVYGLKPSHGRVPYHPLPANGLISHVGPI
                     TRTVADAALMLQAMAGSDDRDMTSISAEPENFLANLDRGIAGLRVAYSPDLGYLRVDP
                     EVAGPVKAAVRAFEELGCQVEEVNPGWGDPIETEHCLFAAHIAGMYGDFLEDWADKMD
                     PGLVAIIRHGMTYSAVDYCKAYAQRLAYYDKVRDFFERYDLLLTPTLSVAAFPAERLI
                     PAHWEQHDWDWLRWAGFSYPFNLTWLPAATCPCGFTPAGLPIGLQIVAGRNRDMRVLQ
                     ASRAFEQARPWAQRRPPP"
     gene            complement(2856..3686)
                     /locus_tag="VE030_02045"
     CDS             complement(2856..3686)
                     /locus_tag="VE030_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22227.1"
                     /translation="MQPILGSVLIAAAWLCWDVPIASAQQGLNYSFDSVARFDAAANR
                     KALWIKGEVAEGDLDRLLRFLREHPRDFVQHGGRVVFVVDGGDVVEATRIGRFLRDAL
                     IEAWLPDASRTRCVSACFFMFVNAVSRVAVADSVGVHRPYFDAGRLAGADPESVRRRY
                     ESLLHEVRDTLDKLLVPRDLTEKMLATPWNDTYWFSREDLDRLGETQAWFEDFSAAKC
                     GVEPRLKQRLESALAAGFEAEAQALRTELDAATICVAELRNRQRQHIVEKLTNDSGSK
                     "
     gene            complement(3715..4326)
                     /locus_tag="VE030_02050"
     CDS             complement(3715..4326)
                     /locus_tag="VE030_02050"
                     /inference="COORDINATES: protein motif:HMM:NF016239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HZF22228.1"
                     /translation="MNYLAHLYLSEPHEEAWLGSLMGDFVKGPLDGRYGSEITRAIAM
                     HRKIDVFTDAHPVVLQSKSRMSTERRRYAGIMVDMFYDHFLARHWQDFHDEPLAEFTG
                     RVYRVLERYHTILPERLQRMAPMMAQWDWLGSYADIGSIYAALNRMGQRLKRENRLMN
                     SADELVGNYAQLEADFRAFLPDVLRYARGRDARPGLAQPTLRR"
     gene            complement(4443..5762)
                     /locus_tag="VE030_02055"
     CDS             complement(4443..5762)
                     /locus_tag="VE030_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZF22229.1"
                     /translation="MVPLPTRLSRLAVPLFLLFAPVAVGADLRTDIDRMADQFEPRVI
                     EWRRDIHQNPELGNREFRTAGKVAGHLKALGLEVRTGIAHTGVVAVLRGGKPGPVVAL
                     RADMDALPMTEEVDLPFASKVRTTWAGKETGVMHACGHDTHVAMLMGAAEILTKVRER
                     LPGTVKFIFQPAEESPPPGEEGGAALMIKQGVMENPRPDVVFGLHIGSMYETGTIAYR
                     PGPVMAAADILHITVQGRQTHGSAPWTGVDPIVVAAQIVMGLQTIVSRQINVTQEPAV
                     VSIGGIEGGNRFNIIPDKVEMIGTIRTFDEAMRDDIHRRVKTTAEHIAEAAGAQATVV
                     IEKPYAVTVNDPDLTERMLPTLKRVAGADNVKLRGKTTGAEDFSFFAQKAPGLFVFLG
                     GSPKGTDLTKVAYNHSPRFYVDESALKLGMRSLVHLTVDYMESPQKR"
BASE COUNT         1112 a         1913 c         1862 g         1077 t
ORIGIN      
        1 gcaaggccag gacaccatcg agacgactta ttttgccagc gccaaccgcg gcaagaaatc
       61 cgtcaccgtg gatatcaccc gcggcgaagg acaggaactg gtgcgcaagc tggccgcgaa
      121 atgcgacgtg ttcatcgaga atttcaagac cggcacgctt tcccgttacg ggcttgccta
      181 tcagcagctc aggcaggcgc gccaggacat ggtctattgc tcgatcacgg gtttcggcca
      241 gaccggccct tacgccgtgc gccccggcta cgacccgatc gcgcaggcgt tgagcggact
      301 gatgagcgtg accggagaat ccgaggacat gccgggtacc gtgccgcaac gagtcggtgt
      361 cgcggtgatc gatctgatga ccgcgcatta tgcggtgatt gcggtggtct cggcgctgta
      421 ccaccggcag gccacggacc ggggacagta catagacatg tcgttgctgg acgtcggcgt
      481 agcctcgatg gcaaacatcg cgtcggctta cctcggcgcg ggcgtgatcg cgaagcgcaa
      541 tggcggcgtg catcccagcg tggtgccatc ccaagtattt ctctgcaaag acggctacgt
      601 gatcgtcgcc gcgggcaacg atggccagtt cgccaagttg tgcgaagtgg ggagcaaggg
      661 cgatctggtc cgtgatgcgc gctttagcac caattcggcg cgggtcaaaa accgggatgc
      721 attgaggccg ctgctcgaag cgatgttcct caagcatccg gtgacctggt ggaacgcaaa
      781 acttgccgcg gccggcgtgc cttgcgcacc gatcaacgac atcgcccagg tgttcgagga
      841 tccccaggtc caacaccgcg gattaagggt cgaaatgccg gagccggccg ccggcaaggc
      901 cgcgttcgtc gccagcccgc tgcgtttttc cgaatcgccg gtcgagtatc ggctgccgcc
      961 gccgttgctc ggggagcaca accaggaagt atttgaagat ctgctgggaa tgtcgccgga
     1021 agaaatcgcg cggctgcgtg catccggcgt gatatagcag ccgcatgtat catatttcca
     1081 tgacgatcga tcccgctgcg atacctgtcg tgcacaacac ggcagccaag cgtttcgaaa
     1141 tcaccatagg cggcaagacg gcatactcga agtacctgct agccggggac aagatcatca
     1201 tcgagcatac cgaggtgccg gaggcgctcg aaggccgggg cctggcaagc cggatggtca
     1261 gcaccgcgct cgattacgcc cgggcgaaga acctgaaggt actgccgatc tgcccgttca
     1321 cgaagagctt catcggccgg catagcgagt accaggacct gctcgcgtga ggctatctct
     1381 acccgaacca acgagccagg ctaatacgca ataaagaact gcaaagatgg ccgacgaaga
     1441 cctttgcttt accccggcta ctgaactgac gcgcatgatc cgcgacaggc tggtctcgcc
     1501 tgtcgaagtg atgaaggtca cggttgagcg tgcccagaag ttgaatacca ggctcaacgc
     1561 catctgcacg ccgacctacg atcgggcgat ggcggcggcg cgcgacgcgg aagccgcgct
     1621 gatgcgtggc gccgcgctgg gccctctgca cggcatcccg accagcatca aggatctcgc
     1681 cttcaccaag ggcgtgcgga cgatggcggg atcgcacatc ttcagcaacc gcgttccgga
     1741 tttcgatcac gttcatgttg cgcggctgcg cgcagccggc gggatttcga tcggcaagac
     1801 cacgacatcg gaattcggct ggaccggcgt gagccgcagc ccgctcaccg ggattaccca
     1861 taatccgtgg aagcacggca tgaacgcggg tgcgtcgagt gccggagcgg cggtatgtgc
     1921 ggcggcgggg atcggcccga tccaccaggg ctcggacggc gccggttcga tccgcatgcc
     1981 ggcgtcgttc tgcggcgtat acggactcaa gccctcgcac ggacgcgttc cttaccaccc
     2041 gttgccggcc aacggtttga tttcgcacgt cgggcccatc acgcgcacgg tcgccgacgc
     2101 ggcgctgatg ttgcaggcga tggcgggctc cgacgatcgc gacatgacca gcatctctgc
     2161 cgagcccgaa aacttcctgg cgaatctgga ccggggcatc gccggcctca gggttgcgta
     2221 cagcccggat ctcggttacc tcagggtcga tccggaagtc gccgggccgg tcaaagcggc
     2281 ggtgcgcgcc ttcgaagaac tcggctgcca ggtcgaagag gtgaatccgg gatggggcga
     2341 tccgatagaa actgaacact gcctgttcgc ggcccacatc gctggaatgt acggcgattt
     2401 tctcgaggac tgggcggaca agatggatcc cgggctggtg gcaatcatcc gccacggcat
     2461 gacctacagc gcagtggatt actgcaaagc gtacgcgcag cgcctcgcct actacgacaa
     2521 ggtgcgtgat ttcttcgaac gctacgatct gctgcttaca cccacgctgt cggttgccgc
     2581 gtttccggcg gagcgcctga ttcccgcaca ctgggaacag cacgattggg actggttgcg
     2641 ctgggccggc ttcagctatc cgttcaatct gacgtggctg ccggcggcca cctgtccctg
     2701 cggcttcacc ccggccgggc tgccgatcgg cctgcagatc gtcgccggtc gaaaccggga
     2761 catgcgcgtg cttcaggctt cccgcgcttt cgaacaggcc cgcccatggg cgcagcgacg
     2821 accgccgccc tgaaactgca atacggttcc ggcgcttatt tgcttcccga atcgttcgtc
     2881 agcttctcga cgatatgctg ccgctgccga ttgcgcagct ccgcaacgca gatcgttgct
     2941 gcgtccagtt cagtccgcag cgcctgagcc tccgcttcga acccagccgc gagggcgctc
     3001 tccaggcgct gcttcaggcg cggctcgaca ccgcacttgg ctgccgagaa atcctcgaac
     3061 caggcctgcg tttcccccag gcgatcgagg tcctcccgtg aaaaccagta ggtgtcgttc
     3121 catggcgttg ccagcatctt ctccgtaagg tcgcgcggca ccaacaattt gtccagggta
     3181 tcgcgtacct cgtgcagcag cgattcgtaa cgccgccgca ccgattcagg atccgcgccc
     3241 gccaggcgcc cggcatcgaa atacggcctg tggacgccga ccgaatcggc gaccgcaacc
     3301 cgcgaaaccg cattgacgaa catgaaaaag cacgcgctga cgcagcgcgt acgcgaagcg
     3361 tcgggaagcc acgcctcgat caacgcgtcc ctgaggaacc ggccgatgcg ggtagcctcg
     3421 acgacgtcgc ccccatccac gacgaagacg acacgcccgc cgtgttgcac gaaatcccgg
     3481 gggtgttccc ggagaaaccg gagcaaccgg tccaggtctc cctcggcaac ttcacccttt
     3541 atccacaacg ccttgcggtt cgcggcagcg tcgaaacgcg cgaccgaatc gaaggagtaa
     3601 ttcaggccct gctgtgcaga ggcgatcggc acgtcccagc aaagccaggc cgccgcaatg
     3661 aggacgcttc caaggatagg ttgcaccgcc gcgcctgtct cgactgtgcc cggatcaacg
     3721 ccgcagggtc ggttgcgcta atccgggccg ggcgtcgcga ccacgcgcat aacgcagcac
     3781 atcggggaga aacgcacgga aatccgcttc cagttgtgcg tagttcccga ccagttcatc
     3841 cgcagagttc atcaggcggt tctcgcgttt cagccgctgc cccatccggt tcagcgccgc
     3901 gtatatcgag ccgatgtcgg catacgaacc gagccagtcc cattgcgcca tcatcggcgc
     3961 catgcgctgc agccgctccg gaagaatcgt gtgatagcgc tccagtaccc ggtaaacgcg
     4021 gccggtgaat tcggccagag gctcgtcgtg aaaatcctgc cagtgcctgg ccaggaagtg
     4081 gtcgtagaac atgtccacca tgattcccgc gtaacgcctg cgctcggtgc tcatccgcga
     4141 cttgctttgc agtaccaccg ggtgggcgtc ggtgaacacg tcgatcttgc ggtgcatggc
     4201 gatggcgcgg gtgatctcgc ttccgtagcg gccatcgagc ggtcccttca cgaagtcacc
     4261 catcaggctg cccagccacg cttcttcgtg cggttcggaa agatagaggt gggcgaggta
     4321 attcatgaat agcagggaag aggggcgagg agagagggac gagggaggaa aaaaatctgg
     4381 tctctaccgc atttcatgat tcatttttct cttccttcgc cccccgtccc tcgtccctgg
     4441 agtcaccgct tctgcggtga ctccatatag tccaccgtga ggtgcaccag cgaacgcatg
     4501 ccgagtttga gcgccgattc gtcgacgtag aagcggggag agtggttgta ggcgaccttc
     4561 gtcaggtccg ttcctttggg cgagccgccg agaaagacga acagccccgg agccttctgt
     4621 gcgaagaagg agaagtcctc cgcgcccgtg gtcttgccgc ggagttttac gttgtccgcg
     4681 ccggccacgc gcttgagagt cggcagcatg cgctcggtca ggtccggatc gttgacggtg
     4741 accgcgtagg gcttttcgat gacgaccgtg gcctgcgcac ctgccgcttc ggcgatgtgt
     4801 tccgcggtcg tcttcacccg ccggtgaatg tcgtcgcgca tcgcctcgtc gaaggtgcgg
     4861 atggtgccga tcatctccac tttatcgggg atgatgttga agcggttgcc gccttctatg
     4921 ccgccgatgg acaccacagc cggttcctgc gtcacgttga tctggcggct gacgatggtc
     4981 tgcagcccca tcacgatctg cgccgcgacc acgatcgggt ccaccccggt ccacggtgcc
     5041 gagccgtgcg tctgccgccc ctgcacggtg atatgcagga tatccgccgc cgccatcacc
     5101 gggcccggac gataggcaat cgttccagtc tcgtacatcg agccgatgtg caggccgaaa
     5161 acgacatccg ggcgcgggtt ctccatcacg ccctgcttga tcatcaatgc ggcgccgcct
     5221 tcctcgcccg gcggcggact ttcctccgcc ggctggaaga tgaatttgac ggttccgggc
     5281 aggcgctcgc gtaccttggt cagaatctcg gccgcaccca tgagcatggc gacgtgcgtg
     5341 tcgtggccgc aggcgtgcat gacgccggtt tccttgccgg cccacgtggt tctgactttc
     5401 gacgcgaacg gcagatccac ttcttcggtc atcggcagcg cgtccatgtc ggcgcgcagc
     5461 gccacgaccg ggccgggctt gccgccgcgc agcaccgcaa ccacgccggt gtgcgcgatg
     5521 ccggtgcgca cctccaggcc aagtgccttg aggtgcccgg ccaccttgcc ggcggtgcgg
     5581 aactcgcggt tgcccaactc ggggttttga tggatgtccc gccgccactc gatcaccctg
     5641 ggctcgaatt gatcggccat gcggtcgata tcagtgcgga gatcggcgcc gactgcgacc
     5701 ggcgcaaaca acagaaacag aggaacggcc aggcggctaa ggcgagtggg taacggcacc
     5761 atgggctcct gtaagacaac gatatggatt ggcgcagtgt tgcctgacat cgccgtgcag
     5821 tcaatccgcc ggcgaccagc cgattctcag ctcaggacca ggccggcgac gtaccagatc
     5881 agcaacaaga ccagggtgcc gcacaccgca taaataccca ggcggaaccg ccggtcttcc
     5941 tgctcttcgc gttccagcgc gtcg
//
LOCUS       DASQOP010000055         9529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2034270, whole genome shotgun sequence.
ACCESSION   DASQOP010000055 DASQOP010000000
VERSION     DASQOP010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9529
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2034270"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="VE030_02060"
     CDS             complement(<1..1041)
                     /locus_tag="VE030_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HZF22230.1"
                     /translation="MMQSAAGGPVLGALPRVVIAGAFVAMALPAIGDPAYFPGGQWER
                     RAPAAAGFDPAALDGAIRYAQQNEAPWERDVFKQIQKDVANEPYPAILGETKERGGPA
                     GMIVRHGYLVAQWGDIDRVDMSFSVAKSYLSTVAGLAVDAGLITDVNDRVADYVQDGG
                     FDDSRNRKVTWHMLLNQTSEWEGTLWDKPDVADRRRGYDRRLQAPGTFWEYNDVRVNR
                     LALALLRVYQRPLPEVLKEKVMDPIGASDTWVWHGYRNSYVDIGGRRVQSVSGGSHWG
                     GGFWASTRDHARFGYLLLRRGEWNGKRILSERWVDLATQPTAIAPHYGYLWWLSRNRR
                     GIFPGAPESSFFA"
     gene            1184..2254
                     /locus_tag="VE030_02065"
     CDS             1184..2254
                     /locus_tag="VE030_02065"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZF22231.1"
                     /translation="MLTRTNIGLTIEQDELKGFSRTIAEIEWLLLALVLVYLLAGATQ
                     DEGGIALNMALFFFAAFILGLHYVQFYKEESRLKLLFETWVMIVFITWVVLYTGSIHS
                     PLLNLYLLPIIASALIFGKLVTAIEVAAIIGCFMFFAYDPGSKTLLSLRYWGEILAMS
                     TPVILVAYITTMLAADIRYAVDKIKQVSDTDELTGLYNMRAFSAMLQRSFKQAVRYGH
                     ALAVVMIDSDNLKQVNDAHGHEAGNRLLQHLVRCVREQLRGSDVMARFGGDEFILLLP
                     ETNNKGALEMSERIRKAVELSRFSVRDGDTNVTVSLGVSSYPEDGGNLDVILEKADKA
                     MYRAKQKGRNRVVSYLEDSTAA"
     gene            complement(2227..3060)
                     /locus_tag="VE030_02070"
     CDS             complement(2227..3060)
                     /locus_tag="VE030_02070"
                     /inference="COORDINATES: protein motif:HMM:NF022673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3025 domain-containing protein"
                     /protein_id="HZF22232.1"
                     /translation="MADFWNTDWLNRSEMFVPLRPVAVGLPAIGWPDLDLLNALAEDR
                     GRRVVNAQGMRVRFVAQAPKPKSFEAAFEPRAYLRGEVQVRAFDWHDLFNALVWMTFP
                     TTKAVINARHYESMAAAGGGNRPPQRDALTLFDEDGVAVVSTDAELLDLVRDFRWKEL
                     FWHRRDEVEARMRFRVFGHALFSKAMQPFVGLTGKAVLLQVPGAFLELAQAAQLAELD
                     RLLAMHIWDRERFRHGRELSPLPVLGVPGWWSGNEREGFYDDTAYFRPGRRQAAVLSS
                     R"
     gene            3123..4229
                     /locus_tag="VE030_02075"
     CDS             3123..4229
                     /locus_tag="VE030_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZF22233.1"
                     /translation="MTERVDCAVIGAGIVGLAVARELALNGREVIVIEAADAIGTHTS
                     SRNSEVIHAGLYYGKGSFKARFCTAGKQLLYAYCGERGVPHRSIGKIVIAASDDEIEA
                     LKGYVVKAEANGVTDLRWLSRAQLRELEPDVDCVAGFLSPSTGIVDSHALMLAYQGDA
                     QNHGAAVVFKSPVRSGEVGDDGIVLDVGGDEPMTIACSAVVNSAGLFAQDVARSIRGI
                     SAQSIPPQYFAKAHYYTLSGKPPFRRLVYPVATSAHLGVHVTVDLAGQVRFGPDVSWV
                     DKVDYSFDHSREPLFYRAIRKYYPGLKDGQLQPGYTGIRPKVSGEGAPAADFVIQGPK
                     EHGIPGLVNLYGIESPGLTASMAIAQHVRTLLQD"
     gene            4263..5570
                     /gene="paaK"
                     /locus_tag="VE030_02080"
     CDS             4263..5570
                     /gene="paaK"
                     /locus_tag="VE030_02080"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HZF22234.1"
                     /translation="MGAKDQTEPIEKAGVDELRALQLKRLRRSLQHAYEKVPHYRKAF
                     DTAGVRPGDLKDLADLERFPFTTKADLRANYPFGMFAVPMDDIVRVHASSGTTGKPTV
                     VGYTKEDVDTWATVMARSLRAAGGKSSDIVHVAYGYGLFTGGLGAHYGAEALGATVIP
                     VSGGMTERQVQLMRDFKPGILMCTPSYALVIADEFVRQGLDPAQCTARVGIFGAEPWT
                     ESMRAEIEKRLGLAALDHYGLSEVIGPGVAAEHIETRDGLTVWEDHFYPEVIDAQTGA
                     VLRDEQTGELVLTSLTKIGMPVIRFRTRDLTRLLPGTARSMRRMARVTGRSDDMLIIR
                     GVNVFPSQIEELLLKQVEFAPHYVLEVRREGHLDDLDVLVERRAGAVAPLSVATITAI
                     EQDMERQIKGYIGVTTRVRVVEPGTVQRSQGKAVRVIDKRPKS"
     gene            5600..7024
                     /locus_tag="VE030_02085"
     CDS             5600..7024
                     /locus_tag="VE030_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZF22235.1"
                     /translation="MAAPVTQVPAQESESLQRAGIGNDAVAAWHAAAPRDTGDFAGDC
                     ARYSRFWESSTALLARLPAKAARDARQAEAAERIKQVAREARTRFLRTHVEVVYDRLT
                     HNRSRFIRVEQLVYDAAALVPGLTPTRAQVTAESACLQRDKDGLEIDHGIFVSAALAN
                     PGIGSHLCHAMLLPHAGTAERLAELEKKGRVNLGAAEVFRKGRASHVIGKNPRHLNAE
                     DATTLDAAEIAVDLAILDPHTQIGVLRGDYVHGGKYQGKRVLGSGINLTHLYYGKVPF
                     IWYLQRDLGIVNKLYRGLAHPGMMPDDVNGQTIEMPWIAAVEGFAIGGHCQYLLTMDY
                     VLAARDAFMSLPARKEGIIPGAANLRLPRFTGDRIARQAILYERRLDCDSPEGRLICD
                     EVVAPADMDSAIERVVQNLTSSGVVSAASNRRAFRVTQEPLDLFRNYFAVYALEQAYC
                     HFSPALISNLEQHWNAKNRKLDSK"
     gene            complement(7197..9500)
                     /locus_tag="VE030_02090"
     CDS             complement(7197..9500)
                     /locus_tag="VE030_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HZF22236.1"
                     /translation="MAKYGIGQAMVRREDQRLLYGAGQYIDDLSLPGEVFVAFVRSPH
                     AHARIVSIDTQAARAMPGVVAVLTGADLKADGIGALPDGEGLKRADGKPMCGPPHHVL
                     AVDAARFVGEPVTAVVAQSRSQAEDAAEQVLVEYEELPAVVTIEAATAPGAPQLWPDA
                     PGNIAAQTRFGKKDACDAAFSKAKHITRLALYNQRLVPVSMEPRGSIAEFDAGTGRVT
                     LRTSCQNPAGLQKTLAESCLKMPMGKVRVRVGDVGGGFGMKTQLYPEDVVCAWAARKL
                     GRPVHWRASRSEEFLAGNHGRDQINRAELAFDADGKILGLRVEIVGNIGAHASGPGAV
                     IVVAVGPKVITGVYHVPALDLRGTAVLTNTNLVGAYRGAGRPEAIYLIERLMDQAAAE
                     MKIDPAELRRRNFIQPGAMPYTTPMGEKFDSGNFPHMLDRILEHADWKGFEKRREDSR
                     KRGRLRGRALSTFLEWTGVVHEETVDLHVEADGRIMVYTAMQAMGQGIETSYVQIISE
                     TLDVDPDRIVIVQGDSDIAQGIGSMGSRSLYIGGSAMFTASKDTVEKGKELAADALEA
                     AASDITYDDGRFKVAGTDVGIGLFELAAKQPEKRIAIKTLQKVGGPSWPNSCHVCEVE
                     VDPETGVTEIVRYTTVDDVGRVINPLIVAGQVHGGIAQAVGQALLENTRYDTESGQLI
                     TGSLLDYCLPRADDLPSFATFTDESTPCKINPLGAKGVGELGTVGGTPTVMNALIDAL
                     RPLGVKHIEMPATSEKVWQAIRHAKAA"
BASE COUNT         1740 a         3130 c         2956 g         1703 t
ORIGIN      
        1 ggcaaagaaa ctcgactcgg gagcaccggg gaaaatccct cgcctgttcc gggacaacca
       61 ccacaggtag ccgtagtgcg gggcgatcgc ggtaggctga gtcgccaagt ccacccagcg
      121 ttcggacagg atacgtttgc cattccactc cccccgacgc agtagcaggt aaccgaagcg
      181 ggcgtggtcg cgggtgctgg cccaaaaccc tccgccccaa tggctgcctc cgctcactga
      241 ctgcacgcgc cggccgccga tgtcgacata ggaattgcgg taaccgtgcc agacccaggt
      301 atccgaagca ccgatcggat ccatcacttt ttccttgagc acttccggca gcggccgctg
      361 gtacacgcgc agcaatgcca gcgccagccg gttcacgcgc acgtcgttgt actcccagaa
      421 cgtgccgggt gcctgcaacc tgcggtcata acccctgcgg cggtccgcca cgtccggctt
      481 gtcccagagc gtgccctccc attcgctggt ctgattgagc agcatgtgcc aggtaacctt
      541 acggttgcgg ctatcgtcga agccgccgtc ctgcacgtag tcggccacgc gatcgttcac
      601 atcggtgatc agtccggcat cgactgccag cccggcgact gttgacagat agctcttcgc
      661 cacgctgaag ctcatgtcca cgcggtcgat atcgccccac tgagccacca ggtaaccatg
      721 gcgcacgatc atgccggcag ggccgccgcg ttccttggtc tcgccgagga tcgccgggta
      781 gggttcgttg gccacgtcct tctgaatctg cttgaagacg tcgcgctccc acggcgcctc
      841 gttttgctga gcgtaccgga tcgccccgtc cagggccgcg ggatcgaacc ctgccgccgc
      901 aggcgcgcgg cgctcccatt gccctcccgg gaaataggcc ggatcgccga tagcgggcag
      961 tgccatcgca acgaatgccc ccgcgatcac aacccgcgga agcgcaccca ggaccgggcc
     1021 cccggcagct gattgcatca tttttcacac ctcccttttc gatgatccgc gcagacgacc
     1081 caccggagat cacacgtcaa ctatggaact cgcagccgaa accgtttagt cttaccgcag
     1141 tttgccggcc cctgcggccg tccataaacc tcaaccgggc ctcatgctaa caagaaccaa
     1201 tatcggtctc acgatcgaac aggacgagct caaaggcttt tcgcggacga tagccgaaat
     1261 cgaatggctg ctgctcgctc tggtcctggt atacctgctg gccggcgcaa cgcaggacga
     1321 gggcggcatc gcgctgaaca tggcgctgtt tttcttcgcc gcgttcatcc tcgggctgca
     1381 ttacgtccag ttctacaagg aggagtcgcg gctcaagttg ctgttcgaga cctgggtgat
     1441 gatcgtcttc ataacctggg tggtcctcta cactggcagt atccacagcc cgctgctcaa
     1501 tctctacctg ttgccgatca ttgccagcgc gctgattttc ggcaagctgg tgaccgccat
     1561 agaagtcgcc gcgatcatcg gctgcttcat gttctttgcc tacgatcccg ggtccaagac
     1621 gctgctctcg ctgcgctact ggggcgagat cctggcgatg tccacgccgg tgatcctggt
     1681 ggcctacatc accaccatgc ttgcggccga catccgttac gcggtggaca agatcaagca
     1741 ggtgtcggac accgacgaac tcaccggcct gtacaacatg cgcgccttct cggcgatgct
     1801 gcagcgatcc ttcaagcagg cggtgcgcta tgggcacgcg cttgcggtgg tgatgatcga
     1861 ctcggacaat ctcaagcagg tcaacgacgc gcatggccac gaagccggca accggctgct
     1921 tcagcatctg gtgcgctgcg ttcgcgaaca gttgcgcggg tcggacgtca tggcgcgctt
     1981 cggtggcgac gaattcatcc tgctgctgcc ggagaccaac aacaagggtg cgctggaaat
     2041 gtccgaacgt attcgcaagg cggtcgagtt gtcgcgattc agcgtgcgcg acggcgacac
     2101 caacgtgacg gtcagtctgg gggtatcgag ctacccagag gacggcggca atctcgacgt
     2161 gatcctggaa aaggccgaca aggccatgta tcgcgccaag cagaaaggcc gcaaccgcgt
     2221 ggtgtcctat ctggaagaca gcacggcagc ctgacggcgt cccggccgga agtacgcagt
     2281 atcgtcgtag aacccctccc gctcgttccc gctccaccag cccggcacgc ccagcaccgg
     2341 cagcggagac aattctctgc cgtggcgaaa ccgttcacgg tcccagatat gcatcgccag
     2401 caggcgatct agctccgcca gttgcgccgc ttgagccaat tcaaggaacg cgccgggcac
     2461 ctgcagcagc acggcttttc cggtcagccc gacaaatggt tgcatggcct tggagaagag
     2521 cgcatggccg aacacacgga agcgcattcg tgcctcgact tcgtcgcgtc ggtgccagaa
     2581 caattccttc cagcgaaagt cgcgcaccag gtcgagcagc tccgcatcgg tggaaacgac
     2641 cgccacgccg tcctcgtcga acagggtgag cgcatcgcgc tgcggcgggc ggttgccccc
     2701 gcccgcggca gccatggact cgtaatggcg ggcattgatc acggccttgg tggtcggaaa
     2761 cgtcatccat accagcgcat tgaacaagtc gtgccagtcg aaggccctca cctgcacctc
     2821 gcccctgaga taggcgcgcg gctcgaacgc ggcttcgaat gatttcggct tcggcgcctg
     2881 ggcgacgaag cgcacccgca tgccctgcgc gttgacgacg cgccggccac ggtcttccgc
     2941 aagcgcgttg agcaggtcga gatccggcca gccgatcgcc ggcaggccga cggccaccgg
     3001 gcgcagcggc acgaacatct cgctgcggtt caaccagtcg gtattccaga agtcggccat
     3061 gcagctgaaa agaggtgatc ggttaacatt accgttccag tatatccacg cccgctgcaa
     3121 atatgactga acgcgttgac tgcgcggtaa tcggcgccgg gatcgtcggg cttgccgttg
     3181 cacgcgaact cgcgctcaac gggcgcgaag tgatcgttat cgaagccgcc gatgcgatcg
     3241 gcacgcacac cagctcgcgc aactccgagg tgattcacgc cgggttgtat tacggcaaag
     3301 gctcgttcaa ggcgcgtttc tgcaccgccg gtaaacaact gctctacgcc tattgcggcg
     3361 agcgcggcgt gccgcacagg agcatcggca agatcgtgat tgcggcgagc gacgacgaga
     3421 tcgaggccct caagggttac gttgtcaaag ccgaagccaa cggcgtcacc gatctgcgct
     3481 ggctgtcgcg cgcgcagttg cgcgaactgg agccggacgt cgattgcgtt gccggatttc
     3541 tttcaccgtc cacgggaatc gtggacagcc atgcgctgat gctggcctac cagggcgacg
     3601 cccagaatca cggcgcggcc gtggtgttca agagtccggt ccggtccggt gaagtcggcg
     3661 atgacggcat cgtacttgat gtcggcggcg acgagccgat gaccatcgct tgcagcgcgg
     3721 tggtgaactc ggccggcctg tttgcccaag acgtcgcgcg atcgatccgc ggcatttccg
     3781 cgcaaagcat tccgccgcag tatttcgcca aggcacatta ctacacgctg tcgggtaagc
     3841 cgccgttcag acgcctggtg tatccggtcg cgaccagcgc ccacctgggc gtgcacgtca
     3901 ccgtggatct cgccgggcag gtgcgcttcg gtccggacgt cagctgggta gataaagtgg
     3961 actacagttt cgaccactcg cgcgaaccgc tgttctaccg cgccatccgc aagtattacc
     4021 cggggttgaa ggacggccag ttgcaacccg gttacaccgg catccgcccc aaggtctcgg
     4081 gtgaaggggc acccgctgcc gacttcgtga tccaggggcc gaaagagcac ggcattcccg
     4141 ggctggtgaa tctgtacggc atcgaatcgc cggggctcac cgcttcgatg gcgatcgcgc
     4201 agcacgtgcg cacgctgctg caggactaaa ctacgaggaa aacccgacac cgaaggaaaa
     4261 acatgggtgc aaaggatcag accgaaccca tcgaaaaagc cggagtcgat gaactgcgcg
     4321 cgctgcagct aaagcggctg cgccggagcc tgcagcacgc gtatgagaag gtaccgcact
     4381 accgcaaggc cttcgacact gccggcgttc gccccggcga tctgaaggat ctggcggacc
     4441 tggagaggtt tccgttcacc accaaggcgg atctgcgcgc caactaccct ttcgggatgt
     4501 tcgccgtgcc catggacgac atcgtgcgcg ttcacgcctc cagcggcacg acgggcaagc
     4561 ccactgtcgt cggctacaca aaagaagacg tcgatacctg ggcgacggtg atggcgcgct
     4621 cgctgcgggc cgcaggcggc aaatccagcg acatcgtcca cgtcgcctac ggctacggcc
     4681 tgttcaccgg cgggctgggc gcgcactacg gcgccgaggc gctgggcgcc accgtgatcc
     4741 cggtgagcgg cggcatgacc gagcgccagg tgcagttgat gcgcgacttc aagcccggca
     4801 tcctgatgtg cacgccgtcc tacgcgctgg tgatcgcgga cgagttcgtg cgtcagggcc
     4861 tggacccggc gcaatgcact gcgcgcgtgg gcatcttcgg ggccgaaccg tggaccgaga
     4921 gcatgcgcgc cgaaatcgaa aaacgtctcg ggctggccgc gctcgaccac tacgggcttt
     4981 ccgaggtgat cggcccggga gtagccgcgg agcatatcga gaccagggat gggctcaccg
     5041 tctgggagga ccacttctac ccagaggtca tcgatgcgca gaccggtgcg gtgctgcgcg
     5101 atgaacaaac cggtgaactc gtcctcactt cgcttaccaa gatcggcatg ccggtgatcc
     5161 gtttccgcac ccgcgacctg acccggctgc tgcccggcac cgcgcgcagc atgcgccgga
     5221 tggcgcgcgt caccgggcgc agcgacgaca tgctgatcat ccgcggcgtc aatgtttttc
     5281 cctcgcaaat cgaggaactg cttttgaagc aggttgagtt cgcgcctcac tatgtgctgg
     5341 aagtgcgtcg cgaagggcac ctcgacgatc tggacgttct ggtcgaacgc cgggccgggg
     5401 ccgtggcacc tttgtcggtt gcgacaatca ccgccatcga gcaggatatg gagcggcaga
     5461 tcaagggcta catcggggtg accacccggg tacgcgttgt cgaaccgggt acggtccagc
     5521 gctctcaggg caaggcggtg cgcgtcatcg acaagcggcc gaagagttga aagtccagga
     5581 gcattgaaag gggcaggatt tggcggcacc agtcacgcaa gtacccgctc aggaatccga
     5641 gagcctgcag cgcgccggca tcggcaacga tgcggtcgcg gcgtggcacg ccgcggcacc
     5701 gcgcgacacc ggtgatttcg ccggcgactg cgcccgttac tcgcgattct gggagtcgag
     5761 taccgccttg cttgcgcgcc tgcccgcaaa ggcggcgcgc gacgcacggc aggcggaggc
     5821 agccgagcgg atcaaacaag tggcgcgcga ggcgcgcacc cgattcctgc gcactcacgt
     5881 cgaggtggtc tacgaccggc tcacgcacaa ccggtcgcgc ttcatccggg tcgagcaact
     5941 cgtttatgac gccgcggcgc tggtgccggg gctgacgccc acgcgcgcgc aggtcacggc
     6001 cgaatctgcc tgccttcagc gcgacaagga cgggctggag atcgaccacg ggatcttcgt
     6061 ctctgccgcg ctggcaaatc ctggcatcgg aagccacctc tgccacgcga tgctgctccc
     6121 ccacgccggg acggccgagc ggctggccga gctggaaaag aaagggcgag tgaatcttgg
     6181 tgcggccgaa gtcttccgta agggccgcgc ctcgcacgtg atcgggaaga atccccgcca
     6241 tctgaacgcc gaagacgcga ccacactcga cgccgccgag atcgcggtcg atctggcgat
     6301 cctcgatcca cacacgcaga ttggcgtgct gcgcggcgac tacgtgcacg gcggcaagta
     6361 ccagggaaag cgcgtgctcg gcagcggcat caacctgacc catctttact acgggaaggt
     6421 gccgttcatc tggtatctgc aacgcgatct ggggatcgtg aacaagctct atcgcggcct
     6481 ggcccacccc gggatgatgc cggacgacgt caacgggcag accatcgaga tgccctggat
     6541 cgcggcggtc gagggattcg ccatcggcgg gcattgccag tacctgctga ccatggacta
     6601 cgtgctggcc gcgcgggacg cgttcatgag cctgccggca cgcaaggagg gcatcattcc
     6661 cggcgctgcc aacctgcggc tgccgcgttt caccggcgac cgcatcgcgc ggcaggcgat
     6721 cttgtacgag cgccggctcg actgcgacag tccagagggc cggctgatct gcgacgaagt
     6781 ggttgcaccg gcggacatgg acagcgcgat cgaacgcgtg gtccaaaatc tcaccagttc
     6841 cggcgtggta agcgccgcgt cgaaccggcg cgccttccgc gttacccagg aaccgctcga
     6901 cctgttccgc aattatttcg ccgtgtacgc actcgaacag gcctattgcc acttcagtcc
     6961 cgcgctgatt tccaacctcg agcagcactg gaacgcgaaa aaccgcaagc tggactcaaa
     7021 atagaatcct tgccacagag aacacagagg aaaggcgaga aaaagtattg tcaaagtgca
     7081 aatgcaggac aaagtccgcg tttccttgcc catggtcttg cccgtcgcac caaccgaatc
     7141 ttgcacttct tgtgttcttc tctgtgtcct ctgtgttctc tgtggctaag cgtctttcat
     7201 gccgccttgg cgtggcggat cgcctgccac accttttcgg aggtggcggg catttcgatg
     7261 tgcttcacgc ccaacgggcg cagcgcgtcg atcaatgcat tcatcacggt gggcgtgccg
     7321 ccgacggtgc cgagctcacc gacacccttt gcgccgagcg ggttgatctt gcacggggtg
     7381 gactcgtcgg tgaaagtggc gaagctgggc aaatcgtctg cacgcggcag gcagtaatcg
     7441 agcagcgaac cggtgatgag ctgcccggac tcggtgtcgt aacgcgtgtt ctccagcagc
     7501 gcctgtccga ccgcctgcgc aattccgcca tgcacctggc cggcaacgat caggggattg
     7561 atcacccgcc cgacgtcgtc cacggtggta tagcgcacga tctcggtcac gccggtctcc
     7621 ggatcgactt cgacttcgca gacatggcaa ctgttcggcc acgacggacc gcccaccttc
     7681 tgcagcgtct tgatggcgat gcgcttctcg ggctgctttg cggccagttc gaacaggccg
     7741 atgccgacat cggtgccggc tactttgaaa cggccgtcgt cataggtgat atcggatgcc
     7801 gccgcttcga gcgcatccgc ggcgagctcc ttgcctttct cgacggtgtc ctttgaagcg
     7861 gtgaacatcg ccgagccgcc gatgtaaagc gagcggcttc ccatgctgcc tatcccctgc
     7921 gcgatgtcgc tgtcgccctg gacgatgacg atccggtccg ggtcgacgtc gagcgtctcg
     7981 gaaatgatct gcacgtagct ggtttcgatg ccctgtccca tcgcctgcat cgcggtatag
     8041 accatgatcc gcccgtcggc ctccacgtgc aggtccaccg tttcctcgtg cacgactccg
     8101 gtccactcca gaaaggtgga aagagcacgg ccgcgcagcc tgccgcgctt gcgcgagtct
     8161 tcacggcgtt tttcgaagcc cttccagtcg gcatgctcga ggatgcggtc gagcatgtgc
     8221 ggaaagttgc cgctgtcgaa cttttcgccc atcggcgtcg tgtagggcat ggcgccgggc
     8281 tgaatgaaat tgcgccggcg cagttccgcc ggatcgattt tcatttccgc cgccgcctgg
     8341 tccatcagcc gctcgatgag atagattgcc tccggccgtc ccgcgccgcg gtacgcgccg
     8401 acgagattgg tattggtcag cactgccgtg ccgcgcaggt cgagcgctgg gacgtgatag
     8461 acaccggtga tcactttcgg tccaaccgcc acgacgatga ccgcgcccgg accactggca
     8521 tgcgccccga tgttgccgac gatctccacg cgcaagccga ggatcttgcc gtcggcgtcg
     8581 aaggccagtt ccgcacggtt gatctggtcg cgcccgtgat tgccggcgag aaactcctcg
     8641 ctgcggctgg cacgccagtg caccggccgg ccgagtttgc gcgccgccca ggcacacacc
     8701 acgtcttcgg gatagagctg ggtcttcatg ccgaaaccac caccgacgtc accgacgcga
     8761 acccgcacct tgcccatcgg cattttgagg caggactccg ccagcgtctt ctgcagcccg
     8821 gcggggttct ggcaactggt ccgcagcgtg acgcggccgg tcccggcatc gaattccgcg
     8881 atgctgccgc gcggctccat gctcaccggc accagccgct ggttgtagag cgcaagcctg
     8941 gtgatgtgtt tggccttgct aaaggcggcg tcgcatgcgt ctttcttacc gaaccgggtc
     9001 tgcgcggcga tgttgcccgg cgcatccggc cacagttgcg gcgcacccgg cgcggtagca
     9061 gcctcgatcg tcacgaccgc cggcaactct tcgtactcga ccagcacttg ctccgcggcg
     9121 tcctccgcct gcgaacggct ttgcgcgacg accgcggtaa ccggctcgcc gacgaaacgt
     9181 gccgcatcca cggcaagcac gtggtgcggc gggccgcaca tcggcttgcc gtcggcgcgt
     9241 ttcagcccct ctccgtccgg cagcgcaccg atgccatcgg ccttgaggtc cgctcccgtc
     9301 agcacggcca ccacgccggg catggcccgc gcggcctgcg tgtcgatgga aacgatgcgg
     9361 gcgtgcgcgt gaggtgaacg cacgaacgcc acaaaaactt cgccgggaag cgacaggtcg
     9421 tcaatgtact gacccgcgcc gtaaagcagc cgctggtctt cgcgccgcac cattgcctgg
     9481 ccgataccgt actttgccat tcgcctgatc cttttcttat gcgttgccc
//
LOCUS       DASQOP010000056         2747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_827256, whole genome shotgun sequence.
ACCESSION   DASQOP010000056 DASQOP010000000
VERSION     DASQOP010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827256"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(268..474)
                     /locus_tag="VE030_02095"
     CDS             complement(268..474)
                     /locus_tag="VE030_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HZF22237.1"
                     /translation="MDPGTSAGKERQVAVTAADLPLHCPLPTQKLWNSHPRVFLPIEK
                     TGQALCPYCGTRYVLKGSVRSTGH"
     gene            complement(494..1414)
                     /locus_tag="VE030_02100"
     CDS             complement(494..1414)
                     /locus_tag="VE030_02100"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HZF22238.1"
                     /translation="MSMADRDGLIWYDGKLVPWREATTHVLTHSLHYGLAVFEGLRAY
                     RTAKGTAIFRLKEHTERLFNSAHIYMMKIPYDRETLMAAQKEVVRANKLDSCYVRPIA
                     FYGSEKMGVSPKGAKVHVAIAAWPWGAYLGSEGLEKGIRVKTSSYARHHVNVSMCRAK
                     YSGTYANSILANMEATEHGYDEGLLLDVDGFVAEGAGENLFMIKNDKIVEPELNSALI
                     GITRESVIQLAGDLGYVIGAKRITRDDLYIADEVFFTGTAAEVTPIREVDGRTVGTGK
                     RGPLTEKIQSMFFDVVNGRSEKYGHWLHYV"
     gene            1582..>2747
                     /locus_tag="VE030_02105"
     CDS             1582..>2747
                     /locus_tag="VE030_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZF22239.1"
                     /translation="MAEPVKTAVIAGRGLIARLGDAAIDANDSEEMKLRKSLLIFASG
                     LMNLAAILWLALYWWMGLKLPTSIPLGFQILSALVVVIYLRTRNFDFFRVAQLSLFLF
                     FPFVIQWSIGSFVSSSGIALLAILAPIGAIVCYGPRESIPWFVAYVVLTIVSGVFDFF
                     LAKGEDYGIPMKTIAVFFVMNFTIVSTMVYLLLRYFIKERIRYEKELAEQHELVRIER
                     EKSERLLVSILPTHIAERLKLQPGTIADGFADVSVMFADVVDFTRLAEELTPNQVVSF
                     LDEVFTRFDKLTAKYSLDKIKTIGDAYMVVGGLAGERLNYVDHVADMALEIIELTQTD
                     PQLRRLAVQLHIGIATGPAIAGVIGATRFIYDLWGDTVNLSSRLTAEAAGGIILV"
BASE COUNT          508 a          822 c          829 g          588 t
ORIGIN      
        1 aggcgggtga gcagggattg cgacaacagc gcatcgccca cccaggacgg cgctatgaca
       61 aggatcctgg gtgccgtgtt atttaccaca gagacacaga ggcgcagagg aaatcaagaa
      121 atgaatcctt gcaataaaaa accgagaagc gagatcgctt agggcgagcc gtactttccg
      181 gcattctgca tccattggcg atggcccatg gcgctttccg accgtttttc ccttctccgt
      241 gtctctgtgt ctctgtggtt caggaattca gtgaccagtg gagcgtacgc tgcccttgag
      301 cacatagcgg gtgccacaat aggggcacag tgcttggcct gttttctcga tcggcaggaa
      361 cacgcgcggg tgcgaattcc aaagcttctg cgttggcaga gggcagtgca gcggcaagtc
      421 ggccgccgtt accgcgacct ggcgctcctt gccggcgctc gtgcccgggt ccatggctgc
      481 ctggtcgcgg cgctcagacg tagtgcagcc agtggccgta cttctccgac cggccgttga
      541 ccacgtcgaa gaacatcgac tgaattttct cggtcagcgg cccgcgtttt cccgtgccga
      601 cggtacggcc atccacttcg cggatcggcg tgacttcggc ggcggtgccg gtaaagaaca
      661 cctcgtcggc gatgtacagg tcgtcgcggg tgatgcgctt tgccccgatc acgtagccga
      721 ggtcgccggc gagctggatc accgattcgc gggtgatgcc gatcagcgcg gagttcagct
      781 ccggttcgac gatcttgtcg ttcttgatca tgaacagatt ttcgccggcc ccttcggcca
      841 cgaaaccgtc tacatccagc aataatccct cgtcgtagcc gtgttcggtc gcctccatgt
      901 tggcgaggat ggagttggca taggtgccgg aatacttggc ccggcacatc gacacgttga
      961 cgtgatggcg ggcgtacgac gaagtcttca cgcggatgcc cttttccagg ccctcgctgc
     1021 ccagataggc gccccacggc caggcggcga tggcaacgtg gacttttgcg cccttggggg
     1081 acacgcccat cttttccgaa ccgtagaagg cgatcggccg cacgtaacag gagtccagct
     1141 tattggcgcg caccacttcc ttttgtgccg ccatcagcgt ttccctgtcg taggggattt
     1201 tcatcatgta gatgtgcgcc gaattgaaca gccgttcggt atgctccttc aggcggaata
     1261 tggcggtgcc tttggcggtc cggtaggcgc gcaatccctc gaataccgcg agtccgtagt
     1321 gcagcgaatg ggtgagcacg tgcgtagtcg cctcgcgcca gggcaccaat ttgccgtcgt
     1381 accagatcag accgtcgcga tcggccatcg acatgtcgct cactccctcg aaaaagcatt
     1441 gattctagcg cagacgcggg gctttgcaga gactgcccgg ttcgccttgc gggaactcgc
     1501 tcgcatgctt tactcttggc ctctttcgaa gcggctggcg ccaagaaaaa caaccggatc
     1561 cgttaaaacg caaccaattc catggctgaa cccgtcaaaa ctgccgtaat agccggacgc
     1621 gggctgatcg cgcgtctggg cgacgcggcg atagacgcga acgacagcga ggaaatgaag
     1681 ctgcgcaagt cgctgctcat tttcgcgagc ggcctgatga acctcgccgc cattctctgg
     1741 ctggcgcttt actggtggat ggggctgaag ctgcccacct cgattccgct gggtttccag
     1801 atcctctcgg cgctggtggt ggtgatctac ctgcgtaccc gaaatttcga tttcttccgc
     1861 gtggcgcaac tgtcgctatt cctgttcttt ccgttcgtca tccagtggtc gataggcagc
     1921 ttcgtgagtt cttcggggat cgcgctgctg gcgatcctgg cgccgatcgg cgccattgtc
     1981 tgctacggcc cgcgcgagtc gattccctgg tttgtcgcct acgtggtgct gaccatcgtc
     2041 tcgggcgtgt tcgatttctt cctcgccaaa ggcgaggact acggcatccc gatgaagacc
     2101 atagcggttt tcttcgtcat gaacttcacc attgtttcca cgatggtgta cctgctgctg
     2161 cgctatttca tcaaggaacg catccgctac gagaaagaac tggccgagca gcacgaactg
     2221 gtgcgcatcg agcgcgaaaa atcggaacgg ctgttggtca gcatcctgcc cacccacatc
     2281 gccgagcggc tcaagctcca gccgggcacg atagccgacg gctttgccga tgtttcggtg
     2341 atgtttgccg acgtggtgga tttcacccgc ctggccgagg agctgacacc gaaccaggtg
     2401 gtgtccttcc tcgacgaggt attcacccgc ttcgacaaac tgaccgcgaa gtacagcctg
     2461 gacaagatca agaccatcgg tgacgcctac atggtggtgg gagggctggc gggagaacgg
     2521 ctgaattacg tggaccatgt cgccgacatg gcgctggaga ttatagagtt gacccaaacc
     2581 gacccgcaac tgcggcggct ggcggtgcag cttcacatcg ggatcgcgac cggcccagcg
     2641 atcgcgggcg tgatcggcgc cacgcgcttc atctacgacc tgtggggcga tactgtcaac
     2701 ctgtccagcc ggctcaccgc cgaggcggcc ggcggcataa tcctggt
//
LOCUS       DASQOP010000057         9198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2439669, whole genome shotgun sequence.
ACCESSION   DASQOP010000057 DASQOP010000000
VERSION     DASQOP010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9198
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2439669"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="VE030_02110"
     CDS             complement(<1..695)
                     /locus_tag="VE030_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZF22240.1"
                     /translation="MPRCDMTGRSRLPGCMARLFIGSVLVGHMGGCALAASKDCKEDI
                     PTLYQRVSPAVVMISGQSINPYKLQDRVSRVIGSGFIFDSKGLILTNSHVAFGRQALY
                     VSMDDGTVLPAKLIGADPIFDIAVIEIPKPEHGTLPTAVLGDSDKIRVGEAVIAIGNP
                     LGLDQTVTRGIVSGLNRILPETPLSLSEPLIQTDAPINPGNSGGPLLNRCGEVIGINS
                     AIIPNAQNIGFAIP"
     gene            complement(786..1304)
                     /locus_tag="VE030_02115"
     CDS             complement(786..1304)
                     /locus_tag="VE030_02115"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HZF22241.1"
                     /translation="MRKLILFIATSLDGYIAGPNGEIDWLFTNQDYGYSEFYATIDTV
                     VMGRKTYEVSRSFGAYPYPGTRAFVFSRTRDGERDDHATFVSGDISRFVKTLKFQPGR
                     NIWLVGGGEIIRPLLQDDLIDEFRIFVHPIVLGTGVPLFPPPLSTKTLRLRDTHAFES
                     GLVRLGYVRVNA"
     gene            complement(1336..2121)
                     /locus_tag="VE030_02120"
     CDS             complement(1336..2121)
                     /locus_tag="VE030_02120"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZF22242.1"
                     /translation="MQDKLFDVADQVVLVSGGSRGIGRALAQGFAERDAQVIVAGREA
                     DTLKQTAKEISRGKHPVAFEVCDVAKAEDVDRLVGSVLKKCGRIDTLLSVAGVNKRMK
                     AEDYTLEEYDRIVNINLRGAFAIAQAVGRHMIGRKAGCIINIDSLNTYAPLAGVTPYA
                     ISKAGVLMMTRALANEWGKHGVRVNSIAPGFFPTDLSKKLWAQEKMGAWGKTVTPLGR
                     LGDVKELVGTAVFLASSAAVFVTGQTIRVDGGVTAGINWPIDL"
     gene            complement(2315..3076)
                     /locus_tag="VE030_02125"
     CDS             complement(2315..3076)
                     /locus_tag="VE030_02125"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HZF22243.1"
                     /translation="MKTLDLNCDMGEGFGAWEMGDDAALLDHVTSANIACGFHAGDPG
                     TIHRTVKLAIDKGVAVGAHPSLPDLQGFGRRSIAVTPSEAYDMVLYQIGALAGFARSL
                     GGRLSHVKPHGALYNMAARDGSLARAIAKAVKDFDSRLVLFGLAGSELVRAGHETGLK
                     VANEVFADRTYQSDGSLTPRSQPNAVIHDVGASIAQVRRMVGEGKVTSIQGADVEVIA
                     DTLCIHGDEPTAVEFARSIRRALADSGIRVAPLAG"
     gene            complement(3126..4130)
                     /locus_tag="VE030_02130"
     CDS             complement(3126..4130)
                     /locus_tag="VE030_02130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HZF22244.1"
                     /translation="MNIRVLKPGVHTTLQDSGRNGYQHYGVPVSGAMDLFSHRVANIL
                     VGNPGDEATLEMTLQGPRLEFDRDTVIAICGGDLSPAVGGAEVPEGKAVRVRAGSVLD
                     FGACRAGCRAYLAVRGGFDVPRVMGSRSTYETAQLGGMHGRALRKDDRLRIGQSAGGI
                     YPDLDAGLQRSKRAFAFPKWAVNQHIEKLGRTPQVVRIIPGRHWEAFPAAAREALTGS
                     EFRIAPDSNRMGFRLDGSVIEPEKPLEILSEAVTFGTIQIPPGGKPIVLMADRQTVGG
                     YPKIAEVASVDLHLLAQLKPGDRLRFELVSLAQAQALWLAREQEIMTIREAVEKHLTA
                     "
     gene            complement(4144..4851)
                     /gene="pxpB"
                     /locus_tag="VE030_02135"
     CDS             complement(4144..4851)
                     /gene="pxpB"
                     /locus_tag="VE030_02135"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HZF22245.1"
                     /translation="MLPNPHLIPMGDRALVIEFGDRLDPELSSRIAAAAQHLRASPPP
                     GVLDIVPAYTTLAVHYDPAVVGAGSTPYEALIEQLEAWLHAQADAELTPGRLVEIPVC
                     YGSGLGEDLEEVGRQHGLTPEEVATIHAGTDYRVYLLGFVPGFAFLGNLDSRIATPRR
                     DAPRPHVPAGSVAIGGNQTGVYPLETPGGWHLIGRTPVEMFTPQAEPPCLIAAGDTVR
                     FVPISRAEFDALSGSRA"
     gene            complement(4874..5518)
                     /locus_tag="VE030_02140"
     CDS             complement(4874..5518)
                     /locus_tag="VE030_02140"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HZF22246.1"
                     /translation="MRGSAGILLSLAAALLLVPAPALAQAETARTFLLVAQPDMLDPN
                     FSRTVVLVMHPEESGPLGVILNRRTNVQLRELYPERAELTGRDDLVFFGGPVQPDALL
                     FAFRSAVKPSKGLSVIGDIYISGFSEVLDELLKHPENANEQRFFAGYAGWAQGQLEYE
                     IARGGWYTLALDVDAIFKMNPLTIYDELVKRATVRRIETRREFRDRTTAVAGRY"
     gene            complement(5654..6922)
                     /locus_tag="VE030_02145"
     CDS             complement(5654..6922)
                     /locus_tag="VE030_02145"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZF22247.1"
                     /translation="MNRLIRSAAACILAGAAFTSHAAALPEAKPEDVGLSSQRLARLD
                     ATMQKAVDSGELPGVVILIARDGRLAYARSFGWQDREKKIPMRNDSIFRIYSMTKPIV
                     SVAAMMLVEEGRLGLHEPVSRFIPEFKDMKVGVEVKDAATGASALTLVNAKRQITVQD
                     LMRHTSGLTYGILGQDTPVKKMYRDAGIFSQKWVLADFVKALAKLPLAFEPGTTWEYG
                     HSTDVLGRVVEIVSGQTLDVFLAERILKPLKMVDSGFHVPADKLNRLAQPAPDVYTGK
                     TPELLDVTQPATFFAGGHGMVSTAGDYLRFAQMLENGGELDGARILGPKTVEYMTANH
                     LNPNISPGPTFLPGPGYGFGLGFGTRLEPGQSEWPGTPGEYFWGGYAGTYFWVDPLED
                     LVPVMMSQEPVRRPHYRSLLRNLVYQSIIN"
     gene            complement(6986..8416)
                     /locus_tag="VE030_02150"
     CDS             complement(6986..8416)
                     /locus_tag="VE030_02150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZF22248.1"
                     /translation="MTAPAQVSSARLPAVRLDPNGNRKVDPRAVVALAIPLFVNSSIQ
                     ALLNLTDTWFIGRLSTDATAAVGASYWFLIVAILLFGGAGLAVQTLASQAYGAGDNRA
                     AARAVWAGIWGMLLVAPAFVAIAFCGSTFIGWLGLEPNVAHLAGAYWFPRMLGGMLAV
                     GIWGTTGFFNGIGRTRVTLAIMTFVAVLNAVLNQIFIFELGWGISGSAWASTAAMFGG
                     LILALALFASRIIDREYYSRQTWRPNAAELARLFRVGIPMGLFPAIDVGGLAMFQAMQ
                     VAAGAIGGAATQIVMMLTSLAYLPTMGFALAGTTLVGQSIGAGDKDWAARVGNATIKL
                     AVAYMGLVTVFLAVIGPWLIPLFVNPSDPNAAAVISLGRTLLWFAAGYQIFDGLNLGA
                     GFCLRGAGDMRVPTVAIVFLSWFGFVPFTHMFTFAPGQGWVDFLPQFGYGTVGGWAVA
                     LVYTCMLGTLIYLRWRSGAWRKMDLR"
     gene            complement(8829..>9198)
                     /locus_tag="VE030_02155"
     CDS             complement(8829..>9198)
                     /locus_tag="VE030_02155"
                     /inference="COORDINATES: protein motif:HMM:NF042855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6806 family protein"
                     /protein_id="HZF22249.1"
                     /translation="WTGDVGRSFHQIISGALASLNPFSEEAAAFEVSYYLEDGQDEFS
                     MVFVGPTPETIYEAQRRRMIEDVQNLLSRQFTEPEVTQVSALINQLFERNWKGGTTAA
                     QAGSGSSEVVPMPPGRKHLH"
BASE COUNT         1700 a         2870 c         2914 g         1714 t
ORIGIN      
        1 gggatggcga acccgatatt ttgcgcgttg ggaatgattg cggaattgat gccgatcacc
       61 tcgccgcacc ggttgagcag cgggccgccg gaattgccgg ggttgatggg cgcgtcggtc
      121 tggatgagtg gctcgctgag cgacaacggc gtttccggca ggattctgtt gagaccgctt
      181 acgattccgc gggtgacggt ctggtccagt ccgagaggat tgccgatggc gataactgcc
      241 tcgccgaccc ggattttgtc ggaatcgccc aagacggccg tcggcagcgt gccgtgctcg
      301 ggtttgggaa tctcgatcac cgcgatgtcg aagatcggat cggcgccgat cagttttgcg
      361 ggcagtaccg taccgtcgtc catggacacg tacagggcct gccgcccgaa tgcaacgtga
      421 gaattggtga gaatcaggcc tttgctgtcg aaaatgaatc cggacccgat cacgcggctg
      481 acccggtcct gaagcttgta gggattgatc gactgtccgg agatcatcac cacggccggc
      541 gaaactctct ggtagagagt ggggatgtct tccttgcagt ctttggacgc ggcaagcgcg
      601 caaccgccca tgtgtcccac taagactgat ccgatgaaca gtctcgccat gcagcccggc
      661 aaacggctgc ggccggtcat gtcacatcgg ggcatggctt ctcctggcaa tgactgaatt
      721 gatgacgtgt gaatggggat cgggtgccgg cgcggagtcc ggtcaggcct aaacgcgggc
      781 agggattaag cgttgacccg gacgtagccg agccgcacca gccccgactc gaacgcatgg
      841 gtatcgcgca gacgcaaggt cttcgtggag agcggtggtg gaaagagcgg gacacccgtg
      901 cccagcacga tcgggtggac gaagatccgg aactcgtcga tgaggtcgtc ctgcagcagc
      961 gggcggatga tttcgccgcc gccgaccagc cagatattcc tgccgggttg aaacttcagt
     1021 gtcttgacga aacggctgat gtcgcccgat acgaaagtgg cgtgatcgtc gcgctcgccg
     1081 tcgcgtgtcc tcgaaaatac gaaagcccgc gttcccggat agggatacgc gccgaacgaa
     1141 cgcgatactt cataggtctt ccggcccatg acgaccgtat cgatcgtggc gtagaactcc
     1201 gagtagccgt aatcctgatt cgtgaacagc cagtcgattt cgccattcgg tccggcgatg
     1261 taaccgtcga ggctggtggc gatgaagagg atgagcttgc gcatgaggag tgtcgcgaaa
     1321 tcttcggcct ggtcgttaca aatcaatcgg ccagttgatt cccgccgtca caccgccatc
     1381 aacgcggatc gtctgcccgg tgacgaacac ggcggcggat gaggccagga aaaccgcggt
     1441 gccgaccagt tccttcacgt cgccgagccg gcccagcggc gtgaccgtct tgccccaggc
     1501 gcccatcttc tcctgtgccc acaacttctt cgacagatcg gtgggaaaga aacccggcgc
     1561 aatggagttt acgcgcactc catgcttgcc ccactcgttt gcaagcgcgc gggtcatcat
     1621 cagcacgccg gccttgctga tcgcgtaagg cgtgacgccc gccagcgggg cgtaggtgtt
     1681 gagcgagtcg atgttgatga tgcaaccggc cttgcggccg atcatgtggc ggccgaccgc
     1741 ctgggcgatg gcgaaggcac cgcgcaggtt gatgttcacg atgcggtcgt attcctcgag
     1801 cgtgtagtcc tcggccttca tgcgcttgtt cacacccgcc acgctgagca gcgtgtcgat
     1861 gcggccgcat ttcttcagga cgctccccac cagcctgtcc acgtcttccg ccttggccac
     1921 atcgcatact tcaaaggcga ccgggtgctt gcccctggag atttccttcg cggtttgctt
     1981 gagcgtgtcc gcttcccggc cggcgacgat cacctgcgcg tcgcgctcgg cgaaaccttg
     2041 cgccagcgct cgcccaatgc cgcggcttcc gccggaaacc agcactacct gatccgcaac
     2101 gtcgaatagc ttgtcctgca tttttgtttc tccctttgcg tttaggtctt ccaccacaga
     2161 ggcacagaga cacggagaaa agcagttgca actgctgaaa aaactgtcac gcggtggaat
     2221 gatcggtatc gcgaatatct ggcgttgttg atctttgtcc ggcttgcttg tttttctctg
     2281 cgcctcggtg tctctgtggt gaatcgaatc tgtactagcc cgccaacgga gcgacgcgga
     2341 tgccgctgtc ggcgagcgcg cgccggatgc tgcgggcaaa ttcgaccgcg gtcggttcgt
     2401 cgccatggat gcagagcgtg tcggcgatga cctcgacatc cgcaccctga atgcttgtga
     2461 ccttgccttc gccgaccatg cgcctgacct gcgcgatcga agccccgacg tcgtggatca
     2521 ccgcattcgg ctggctccgc ggcgtcaggc tgccgtccga ttgatacgtg cgatcggcaa
     2581 atacttcgtt ggcgaccttg agtcccgtct cgtgtccggc gcgcaccagt tcgctgcctg
     2641 ccagcccgaa cagcaccagc ctcgaatcga aatcctttac cgccttggcg atggcgcgcg
     2701 ccaggctgcc gtcccttgcc gccatgttgt aaagcgcgcc atgcggcttc acgtgcgaga
     2761 gtctgccgcc cagcgaacgg gcaaatccgg ccagcgcgcc gatctgatac aaaaccatgt
     2821 cgtaggcttc gcttggggtg accgcgatgc tgcggcggcc aaagccctgc aagtcgggca
     2881 gcgaaggatg cgcgccgacg gcgacgccct tgtcgatcgc cagcttcacg gtgcgatgaa
     2941 tggtgcctgg atcgccggca tggaagccgc aggcgatgtt ggccgaggtg acatgatcca
     3001 gcagcgccgc atcgtcgccc atttcccagg cgccgaagcc ttcgcccatg tcgcagttca
     3061 aatccagcgt tttcatcggc tcaatatcaa tttagccaca gaggccagtc ttgcccttga
     3121 tgaactcatg cggtcagatg cttttccacc gcctcgcgga tggtcatgat ctcctgctcg
     3181 cgcgcgagcc agagtgcctg cgcctgcgcc agcgacacta gctcgaatcg cagccggtcg
     3241 ccgggcttga gctgcgccag caaatgcagg tccacgctcg ccacctcggc gatctttgga
     3301 tagccgccca ccgtctgccg gtccgccatc aggacgatcg gcttgcctcc cggcggaatc
     3361 tggattgtgc caaaggtcac ggcttccgac aggatttcca gcggtttctc gggttcgatc
     3421 acggatccgt cgagacggaa gcccatgcgg ttggaatccg gggcgatgcg gaattcgctt
     3481 ccggtgagcg cctcgcgtgc agcggccggg aacgcttccc agtgacggcc ggggatgatc
     3541 cgcaccacct gcggcgtgcg gccgagtttc tcgatgtgct gattcaccgc ccatttcgga
     3601 aacgcaaatg cgcgcttgga acgctggagt ccggcgtcga gatcagggta aatgcctccg
     3661 gctgattggc cgatgcgaag ccggtcgtct tttctgagcg cccgcccgtg catgccgccg
     3721 agttgcgcgg tttcgtaggt actgcggctg cccatcaccc gcggcacgtc gaagccgcca
     3781 cgcaccgcga ggtaggcgcg gcagccggcg cgacaggcgc cgaagtcgag cacgctgccg
     3841 gcgcgcactc gtaccgcctt gccttcggga acctcggcgc cgccgaccgc gggggacagg
     3901 tcgccgccgc agatcgcgat caccgtatcc cgatcgaact cgaggcgcgg cccctgcagc
     3961 gtcatttcga gagtcgcttc gtccccgggg ttgccgacca ggatattggc aacccggtgc
     4021 gagaacaggt ccatcgcacc ggaaaccggt acgccatagt gctgataccc gttgcggccg
     4081 ctgtcctgca gcgtggtatg cacacccggc ttgagaacgc gaatgttcac gcaccgcacc
     4141 tgttcatgca cggctccccg ataaggcgtc gaactcggcg cgcgagatcg gaacgaaacg
     4201 taccgtgtcg ccggcagcga tcaagcaggg cggctcggcc tgcggggtga acatttccac
     4261 cggcgtgcgg ccgatgagat gccagcctcc cggcgtttcc agcggataca cgccggtctg
     4321 gttgccgcca atggctacgc tgcccgcggg aacatgcggg cgcggcgcat cccggcgcgg
     4381 cgtggcgatg cgggaatcca ggttgccgag aaaggcgaag cccggtacga aaccgagcag
     4441 gtacacgcgg taatccgtgc cggcgtgaat cgtggcgact tcttcgggcg tgagcccatg
     4501 ctggcggccc acctcttcca ggtcttcgcc cagtccgctg ccgtaacaga ccggaatctc
     4561 caccaggcgt cccggcgtca gttccgcgtc ggcctgcgcg tgcagccacg cttcgagctg
     4621 ttcgatcagg gcctcgtagg gcgtgctgcc ggcgcctacg actgccgggt cgtagtgcac
     4681 cgccagtgtc gtataggccg gtacgatatc gagcacgcca ggcggcgggg atgcacgcag
     4741 atgttgcgca gcggcggcga tgcgtgagct cagttcggga tcgagccggt cgccgaattc
     4801 gatgaccagt gcccggtctc ccatcggaat caggtgcgga ttgggcagca tggctcgcaa
     4861 ttacggccta gggtcagtac cggcctgcga cagccgttgt tcggtcgcgg aactcgcgcc
     4921 gggtttcgat gcgccgtacg gttgcgcgct tcaccagttc atcgtagatc gtcagcggat
     4981 tcattttgaa gatcgcgtcc acgtccagcg ccagcgtgta ccagccgccc cgggcgattt
     5041 cgtactcgag ctggccttgc gcccagccgg cgtaaccggc aaagaaacgc tgttcgttgg
     5101 cgttctccgg atgtttgagc aattcgtcga gaacttcgct gaaaccgctg atgtagatat
     5161 ccccgattac gctgaggccc ttcgacggtt tcaccgcgga gcggaaggcg aacagcagcg
     5221 catcgggttg caccggcccg ccgaagaaca cgaggtcgtc gcggccggtc aattccgcgc
     5281 gctccggata cagctcgcgc aactggacgt tggtgcgacg gttgagaatg acgccgagcg
     5341 ggccgctttc ttcagggtgc atgaccagca ccaccgtccg ggaaaaattc gggtcgagca
     5401 tatccggctg cgcgaccaac aggaaagtgc gcgccgtttc cgcctgtgcc agcgctggcg
     5461 caggcacgag caacaaagcg gcggcgaggg acagcagaat gccggcagat ccacgcatct
     5521 gcgtagtttt acactctctc cacgctttcg gggagagggc cggggaataa aaacaaggat
     5581 gaaggatgaa gggtgaaggt gaaggtgaag gagaaccatg ctccagcacc gacctgtgcc
     5641 cgtgtcctgc gcattagttg atgatcgact gatacaccag attgcgcagc agcgagcgat
     5701 agtgcggacg gcgcacgggt tcctgcgaca tcattaccgg taccagatcc tcgagtggat
     5761 ccacccagaa gtaggtcccg gcgtagcccc cccagaaata ctcgccgggc gtgccgggcc
     5821 attccgactg tcccggctcg agccgcgtgc cgaagccgag tccgaagccg tagccgggcc
     5881 cgggcaggaa ggtcgggccg gggctgatgt tcgggttcaa atggttggcg gtcatgtact
     5941 ccactgtttt cggcccgagg atgcgcgcgc cgtcgagttc gccgccgttc tcgagcatct
     6001 gcgcgaagcg caggtagtcg ccggcggtag acaccatgcc gtggccgccg gcgaagaacg
     6061 ttgccggctg ggtgacgtcg agcagttccg gggtcttgcc ggtgtataca tcgggcgccg
     6121 gttgcgcgag gcgattgagc ttgtccgcgg gcacgtgaaa gccgctgtcg accattttca
     6181 gcggcttgag aatgcgctcg gcgaggaaca cgtccagggt ctggccggag acgatttcca
     6241 cgacgcgacc gagcacgtcg gtggaatgcc cgtattccca ggtagtgccg ggttcgaagg
     6301 cgagcggcaa cttggcgagc gccttgacga aatccgcaag cacccacttc tgcgaaaaaa
     6361 tcccggcgtc gcggtacatc ttcttgaccg gcgtgtcctg tccgaggatg ccgtaagtga
     6421 gtcccgaggt atggcgcatc aggtcctgta ccgtgatctg gcgctttgcg tttaccaaag
     6481 tcagtgcgga tgcgccggta gccgcgtcct tgacctctac gccaactttc atgtccttga
     6541 actcgggtat gaacctggac accggctcgt gcaagccgag cctgccttcc tcaaccagca
     6601 tcatcgccgc gacgctgacg atgggcttgg tcatcgaata gatgcggaaa atcgagtcgt
     6661 tgcgcatcgg tattttcttt tcgcggtcct gccagccgaa gctcctggca taggcgagcc
     6721 ggccgtcgcg tgcgatcagg atgaccaccc cgggcagttc gcccgaatcc accgcttttt
     6781 gcatggtggc gtcgagccgc gcgagacgct gcgaagacag gccgacgtct tccggcttcg
     6841 cttccggcag cgcggcggcg tgcgaggtga atgcggcgcc ggccagaatg catgcggcag
     6901 ccgaacgaat gagcctgttc atggaactcc tcccgatggt gatgggtcgc ccgtgatcgc
     6961 gccttattgt gtttctctta ccatcttacc gcaggtccat cttgcgccag gccccggagc
     7021 gccagcgcag gtagatgagg gttccgagca tgcaggtgta gaccagcgca accgcccagc
     7081 cgccgacggt gccgtaaccg aactgcggca ggaaatccac ccagccctgt ccgggagcga
     7141 acgtgaacat gtgggtgaag ggcacgaaac cgaaccagga caggaagacg atcgccaccg
     7201 tcggcacccg catgtcgccc gcgccacgca ggcagaagcc ggcgccgagg ttgagaccgt
     7261 cgaagatctg gtagccggcc gcgaaccaca gcagcgtgcg cccgagcgag atgaccgcgg
     7321 cggcgttcgg atcggaaggg tttacgaaca gcggaatcag ccatggtccg atcaccgcca
     7381 ggaataccgt caccagtccc atgtatgcca ccgccagttt gatggttgcg ttacccaccc
     7441 gcgcggccca gtccttgtcg ccggcgccga tgctctggcc gaccagcgta gttccggcga
     7501 gcgcgaagcc catggtaggc aggtaggcga gcgaagtgag catcatcacg atctgtgtag
     7561 cggccccgcc tattgcgccg gcggccactt gcatggcctg gaacatcgcc aggccgccga
     7621 cgtcgattgc cggaaacagg cccatcggga tgccgacgcg gaacagtctg gccagctcgg
     7681 cagcgttggg gcgccaggtc tggcgcgagt aatactcacg gtcgatgatg cggctggcga
     7741 acaaagcgag cgcgagtatc aaaccgccga acattgccgc ggtgctggcc cacgcggagc
     7801 ctgaaatccc ccaacctaat tcgaaaatga atatctgatt caggacggcg ttgagcacag
     7861 ccacgaaagt catgatcgcc agcgtcacgc gggtgcggcc gatgccgttg aagaacccgg
     7921 tcgtgcccca gatgccgacg gcgagcatgc cgccgagcat gcgcggaaac cagtacgcgc
     7981 cggcgagatg cgccacgttt ggctcgagcc cgagccagcc gatgaaggtc gaaccacaga
     8041 atgcaatcgc aacgaatgcc ggcgccacca gcagcatgcc ccatataccc gcccacactg
     8101 cgcgcgcggc cgcgcggttg tcgcccgcgc cataggcctg cgaggccagg gtctgcaccg
     8161 ccagccccgc gccgccaaac agcaggatcg caacgatcag aaaccagtac gacgcgccaa
     8221 ccgctgcagt ggcgtccgtg gagagccggc cgatgaacca ggtgtcggtg aggttgagca
     8281 gcgcctggat gctgctgttg acgaacagcg ggatcgccag cgccactacg gcgcgcggat
     8341 cgaccttgcg gttgccgttc gggtcgagcc tgacggcagg taatcgcgcg gaagagactt
     8401 gtgcaggggc ggtcatgagg ccttacagaa agattgttcg gacattcgtg taggtgatca
     8461 gctcccgggt gttgacgaat tgatcgcccg gcggaaacac tcgtcgccaa accattacgg
     8521 ccaggcgtta accgcaaggg acgccaagga cgccggggaa agccaaatgc aagaatcagg
     8581 tgagggttca acccccgtgc aagcatctgc gttgtgactg gcccgttacc ctatctcggc
     8641 ctcgttcttg gctattgcct tttcttggcg tctctggcgg tggatgcgtc attgctgagg
     8701 cgttcgaaac ccacagaaaa gtctgaagga cctacaaaaa aagcgcccgg ggtgattccc
     8761 gggcgcacgc tgaaagctcc tgtgctttta cctcttcact cattcaccct tcacgccttc
     8821 accgcctttt aatgcagatg cttccttccc ggtggcatcg gcaccacttc ggaagagccg
     8881 ctgcccgcct gtgccgcggt cgtgccacct ttccagttgc gctcgaacaa ctggttgatc
     8941 aatgcagaga cctgcgttac ttcgggttcg gtgaactggc gcgacaacag gttctgcacg
     9001 tcctcgatca tgcgccggcg ctgcgcttcg tagatcgttt cgggagtcgg gccgacaaag
     9061 accatgctga attcgtcctg gccatcctcc aggtagtagc tcacttcgaa ggccgccgct
     9121 tcctcgctga acgggttgag cgaggccagc gcccctgaga tgatctggtg aaagctgcgc
     9181 cccacgtctc cagtccag
//
LOCUS       DASQOP010000058         3904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1634370, whole genome shotgun sequence.
ACCESSION   DASQOP010000058 DASQOP010000000
VERSION     DASQOP010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1634370"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VE030_02160"
     CDS             <1..346
                     /locus_tag="VE030_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22250.1"
                     /translation="LPPTEDDIRILERARALLSDERHWNRQDDRTCRPNPERWSLFCA
                     LMQATEEVSGGVHYRQPALQAAREVLNEVGGNRLGKHRLMDYNNHPATTLEEVHQLLR
                     TAQSRLAKQVPR"
     gene            387..1163
                     /locus_tag="VE030_02165"
     CDS             387..1163
                     /locus_tag="VE030_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22251.1"
                     /translation="MKQALTPSVLRVHWRHFLMVAIVAGSLALMLSQQPFGQDPKYHD
                     FADRRTFFGIPNFLDVTSNLAFLLVGIAGLRICLRNCLGRSRYAWVALFAGVTLVSAG
                     SAYYHWDPTNQTLLWDRLPITIGFMGLFAALLGEYIDEWLGMLVLVPALILGVASVVY
                     WHRFDDLRFYVWVQLIPLLTIPVAMVLYRARYSHRWLLLVALGWYALAKISEANDGQI
                     LALSQGAISGHTIKHLLSALACSAVLLMLQRRKLLATKRT"
     gene            1166..1429
                     /locus_tag="VE030_02170"
     CDS             1166..1429
                     /locus_tag="VE030_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22252.1"
                     /translation="MSRLAAAVVATVFASWAPWAAALDRDGAIEVAKRQVKGKCDSTT
                     PCTFTATTAEGKWHVRVDFTKPGRHAIFIIDQGGKVVGRLEGK"
     gene            1436..1933
                     /locus_tag="VE030_02175"
     CDS             1436..1933
                     /locus_tag="VE030_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HZF22253.1"
                     /translation="MADDKAFSLEGHCTCGAVRYRMTSKPMFVHCCHCTWCQRETGAS
                     FALNAMIEADRVILLKGEPEVVNTPSNSGKGQKIARCPTCRVALWSNYGGGGDVVRFV
                     RVGTLDEPGRLPPDIHIFTSSKQPWVVLPLDTPAVEEYYERDRYWPKESLERRRNLLA
                     SARSR"
     gene            1954..2385
                     /locus_tag="VE030_02180"
     CDS             1954..2385
                     /locus_tag="VE030_02180"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HZF22254.1"
                     /translation="MNTTSGHCLCGAIAFSYEGAPNWTLHCHCDSCRRATSSPMATWI
                     SVPRNAFRFTAGKPTYFASSPGVRRGFCARCGSPLTYENERVPDEIHLLAGALDDPQR
                     VHATAHVFAGEQLPWFDTADELPRYETTRRNAQPIRRGPRK"
     gene            complement(2396..3424)
                     /locus_tag="VE030_02185"
     CDS             complement(2396..3424)
                     /locus_tag="VE030_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZF22255.1"
                     /translation="MLKLDIFPHIFPQPYFERMKQIAEGNPSLAAQLKRWMHIPVLWD
                     LDKRMRMMKRFPEYKQILTLSMPAIEFLGNPQETPELARLANDGMAEIVQKHPRQFPA
                     FVASLPMNNVPASLEEVDRAIGKLGARGIQIFTNVNGRPLDEPEFFPVFERMANKYEL
                     PVWVHPSRTAKFADYPTEPKSKYEIWWLFGWPYETSAFMARLVFSGMLDRLPKLRIIT
                     HHLGAMAPFFVNRIGYGMDQLGARTSDEDYASLLKKMAKRPVDYFRMFYGDTAVNGAR
                     SAIRCGIDFYGTKRVLFGTDCPFDPQGGPLFIRETIKALDGLKLKKDDARRIYFGNAM
                     RMLKLKLK"
     gene            complement(3424..>3904)
                     /locus_tag="VE030_02190"
     CDS             complement(3424..>3904)
                     /locus_tag="VE030_02190"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HZF22256.1"
                     /translation="LIGELFAHRFKLNMTHVPYKGGGAGIADVVSGQIEMLITGIATS
                     VGRIKAGQVRALVMTGGARSPLVPEVPTMADAGVQDFVLGALFGVFVPLNTSGEVIER
                     LSRELPAISRNPDFSKRLVDIGQELAEPLLGEAFGRSIRAEAARWRELSTMAGVKEE"
BASE COUNT          713 a         1215 c         1227 g          749 t
ORIGIN      
        1 gctgccgccg actgaagacg acatcaggat attggagcgg gcccgggcgc tgctgtcaga
       61 cgagcggcac tggaacagac aggacgacag gacctgccgc ccgaatcccg agcgctggag
      121 cctgttttgc gccctgatgc aggccaccga ggaagtttcc ggcggagtgc attaccggca
      181 gccggccctt caggcggcaa gggaagtcct gaacgaagtg ggcggcaacc ggctcggcaa
      241 gcaccggctc atggactaca acaaccatcc ggcgacgacg ctggaagagg tgcaccagct
      301 gctacgcaca gcccaatcgc gtctcgcgaa gcaggtcccg cgctgagacg gttgattggt
      361 ggcattacct tcccatgggc caagcaatga aacaggcact cactccatcg gtgctcagag
      421 ttcactggcg gcattttctg atggtggcga tcgtggcggg gtccctggcg ctgatgctgt
      481 cgcagcagcc gtttgggcaa gacccgaaat atcatgattt cgctgatcgg cggacgttct
      541 tcggcattcc caacttcctc gacgtcacat cgaatcttgc ttttcttctt gttggtattg
      601 ccgggctgag gatttgcctt aggaactgct tgggcagaag tcgctacgct tgggttgctc
      661 tgttcgcggg cgtgaccctg gtcagtgctg gctcggccta ttatcactgg gatccaacca
      721 atcagacgct cctctgggac aggctgccca tcaccatcgg cttcatggga ctgttcgcag
      781 cgctattggg cgaatatatt gacgagtggc ttggcatgtt ggttcttgtg cctgcgttaa
      841 tactgggcgt cgccagcgtg gtctactggc acaggttcga tgacctgcgc ttctatgtct
      901 gggttcagct gatacccctg ctgaccatcc cggtcgcgat ggtcctgtac cgtgccagat
      961 attcacaccg gtggctgctc ctcgtggccc tgggctggta cgcgctcgcg aagatctcag
     1021 aggccaatga tggacagatt ctggccctga gccagggcgc gatcagcggc cacacgatca
     1081 agcatcttct gtcggcgctg gcctgctcag ccgttcttct gatgcttcaa agaagaaagt
     1141 tgcttgcaac caagaggaca tgaaaatgag tcggctagct gctgcagttg ttgcgacggt
     1201 gtttgcctcc tgggctccat gggctgcggc tctcgataga gatggggcga tcgaggtagc
     1261 caagcgccag gttaaaggca agtgcgattc cacgacgccg tgcaccttca ccgctacaac
     1321 cgcggagggc aaatggcatg tacgcgtgga tttcacaaag cccggcaggc atgcgatttt
     1381 catcatcgac caaggcggca aggtggtcgg gcggcttgaa gggaaatgaa gccggatggc
     1441 tgacgataaa gccttctcct tggagggcca ctgcacctgc ggtgccgtgc gctaccggat
     1501 gaccagtaag ccgatgttcg ttcactgttg tcattgcact tggtgccagc gcgaaaccgg
     1561 ggcgtccttc gcgctcaacg ccatgattga agccgaccgc gtgattctgc tgaagggcga
     1621 gccggaagtg gtgaacaccc cttccaacag cggaaaggga cagaagatcg cccgctgccc
     1681 gacttgccgt gtcgccttat ggagcaacta cggcggcggg ggtgatgtcg tgcgcttcgt
     1741 ccgcgtcgga acgctcgatg agccgggtcg cctgccgccc gacatccata tcttcacctc
     1801 gtcgaagcag ccctgggtcg tgcttcctct ggatacgccc gcggtcgagg agtactacga
     1861 gcgcgacagg tactggccga aggaaagcct cgagcgccgc cgcaatctac tggcgagcgc
     1921 gcgcagcaga tgaaacctgc ggagcagact cccatgaaca ccaccagcgg ccattgtctc
     1981 tgtggggcga tcgcgttctc atacgagggc gcgccgaact ggacgctcca ctgccactgc
     2041 gacagttgcc ggcgcgccac gtcctcgccc atggccacct ggatctcggt gccgcggaac
     2101 gcgttccgtt tcaccgcggg gaagcccaca tactttgcct cgtctcccgg cgtgcgacgg
     2161 gggttctgcg cgcgctgcgg ctctccgctg acctacgaga acgagcgcgt gccggatgaa
     2221 atccatctgc tggccggcgc gctggacgat ccgcagcgcg tgcatgcaac ggcgcacgtc
     2281 ttcgcgggag agcagctccc ctggttcgac accgcggacg agctgccgcg ctacgaaacg
     2341 acccggcgca acgcgcagcc tatccggcgc gggccgcgga aataacgacg ctcgctcact
     2401 tgagcttgag tttgagcatc cgcatcgcgt tgccgaagta gatgcgcctc gcatcatcct
     2461 tcttcagctt gagaccgtcc agcgccttga tcgtctcgcg gatgaagagc gggccgccct
     2521 gcgggtcgaa cgggcagtcg gtgccgaaca agactctttt ggttccataa aaatctattc
     2581 cacaccggat cgcggaccgc gccccgttca ccgcggtgtc gccgtagaac atcctgaagt
     2641 agtccaccgg gcgcttcgcc atcttcttca gcagcgacgc gtagtcctcg tccgaggtgc
     2701 gggcgccgag ctggtccatg ccgtagccga tgcggttgac gaaaaacggc gccatcgcgc
     2761 cgagatggtg ggtgatgatg cgcagcttcg gcagcctgtc gagcatgcct gagaacacga
     2821 gccgcgccat gaacgcgctg gtctcgtagg gccagccgaa cagccaccag atctcgtact
     2881 tggacttagg ctcggtcggg taatcggcga acttcgcggt gcgcgacgga tgcacccaga
     2941 ccggcagctc gtacttgttc gccatgcgct cgaagaccgg gaagaactcc ggctcgtcga
     3001 gcggccggcc gttgacgttg gtgaagatct ggatgcccct ggcgccgagc ttgccgatgg
     3061 cgcggtccac ctcctcgagc gacgcgggca cgttgttcat cggcagcgag gcgacgaagg
     3121 ccgggaactg cctggggtgc ttttgcacga tctcagccat gccgtcgttc gccagccgcg
     3181 ccagctccgg ggtctcctgg ggattcccca ggaactcgat ggccggcatc gacagggtca
     3241 ggatctgctt gtactcgggg aagcgcttca tcatgcgcat gcgcttgtcc aggtcccaca
     3301 gcaccgggat gtgcatccag cgcttgagct gcgccgcgag cgacgggttc ccctcggcga
     3361 tttgcttcat ccgctcgaag tacggctgcg gaaagatgtg cggaaaaatg tcgagtttca
     3421 gcattactct tcctttactc ctgccatggt ggaaagctct ctccagcgcg ccgcctcggc
     3481 gcggatgctg cggccgaagg cctcgccgag cagcggctcg gcgagctcct ggccgatgtc
     3541 gacgaggcgc ttgctgaaat cgggattgcg ggaaatcgcg ggcagctcgc gcgacagccg
     3601 ctcgatcact tcgcctgagg tattcaaagg aacgaaaaca ccgaatagcg cgccgagcac
     3661 gaaatcctgc acgcctgcat cggccatcgt cggcacctcc ggcaccagcg gcgagcgcgc
     3721 gccgcccgtc atgacgagcg ccctcacctg gccggccttg atgcgcccga ccgaggtggc
     3781 gatgccggtg atcagcatct cgatctgccc agacacgacg tccgcgatgc cggcgccgcc
     3841 acccttgtac ggcacatgcg tcatgttgag cttgaagcgg tgcgcgaaca gctcgccgat
     3901 cagg
//
LOCUS       DASQOP010000059         6791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1030638, whole genome shotgun sequence.
ACCESSION   DASQOP010000059 DASQOP010000000
VERSION     DASQOP010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6791
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030638"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /gene="trpS"
                     /locus_tag="VE030_02195"
     CDS             complement(<1..703)
                     /gene="trpS"
                     /locus_tag="VE030_02195"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HZF22257.1"
                     /translation="MFVDRVLSGMRPTGSLHLGHYHGVLKNWVRLQHEFQCLFFVADW
                     HALTTHYDTPEVIEQHVWDMLIDWLAAGVDPSQATLFIQSRVPEHAELHLLLSMITPL
                     GWLERVPSYKDQQEKLTEKDLATYGFLGYPLLQSADILIYRANRVPVGEDQVPHIEFT
                     REIARRFNHVYGRESGFEQKAEAAIKKLGVKRGKLYRELRNRYQEQGDEEALAAAKAL
                     LEGAANLSMGDRERLF"
     gene            complement(712..1389)
                     /locus_tag="VE030_02200"
     CDS             complement(712..1389)
                     /locus_tag="VE030_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HZF22258.1"
                     /translation="MTEFESIVRLIALYAIPGIFAITLHEAAHGYAARHFGDLTAYEA
                     GRITLNPIRHIDPIGTILIPALILIASGGKYAFGWARPVPVNFGRLRNPKRDMLWVAA
                     AGPGANLLMAVMWALAIKLAQVMPQNYFTEPLILMARGGIIINVVLMVLNLLPLPPLD
                     GGRIAVSLLPRAAAYRFAMVEPYGMIILLVLMFLGVLGTVMMPFVGAFIGGLAAIFDI
                     GWIFGIR"
     gene            complement(1386..2015)
                     /locus_tag="VE030_02205"
     CDS             complement(1386..2015)
                     /locus_tag="VE030_02205"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006401927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HZF22259.1"
                     /translation="MAQYFSIHVNNPQPRLIRRAVEIVRGGGLMVYPTDSCYALGCHI
                     GDKTAMERIRRIRGVDEHHHFTLVCRDLSELGQYAVVDNAQYRSLKAATPGSYTFILK
                     ATREVPKRLLNPRRKTIGLRVPDHAVVRALLEEMREPILSSTLILPGDETPLNDGEEI
                     RARLEHDVDLILDGGPCGVEATTVVDLTEQAPVIVRQGKGTAAPFGVNA"
     gene            complement(2023..2874)
                     /locus_tag="VE030_02210"
     CDS             complement(2023..2874)
                     /locus_tag="VE030_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008159381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HZF22260.1"
                     /translation="MQSLRIDLHSHSTYSDGVLTPAALIERASNNRVTMLALTDHDEV
                     GGLAEAAAAAREAGIGFVPGVEVSVTWAGLTVHVIGLGIEARNAPLTHGLAGIRLSRG
                     LRAVRIAQQLDAAGIPNSLAGASRLAGDAAVIGRGHFARFLVEQGQAADAKTAFKKYL
                     GMGKCGYVAHQWVSLGQAIAWIRGAGGHAVLAHPDRYRLAPLQMHALLDEFKQHGGQA
                     LELYGGRRANPLPMLRLATHFGFSLSAGSDFHAAAQGPADLGDIPPFPDGARTLWQDW
                     EVLRSAA"
     gene            2991..3773
                     /locus_tag="VE030_02215"
     CDS             2991..3773
                     /locus_tag="VE030_02215"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HZF22261.1"
                     /translation="MCVAVGLVLTMTYLPLAGAEPGCESHSTPRRVAVIELYTSEGCN
                     SCPLADRWVSSLPLRGFNADRVVPLAFHVGYWDYLGWRDRFAQASFSVRQRAQADRGS
                     ARFVYTPQLLLNGADFRQPFLDSVFSERLAKVNSRVAAADLRLVQRADSAGVSIELES
                     RVIEPSAHKFSETYVALLENNLSSEVRAGENSGKVLLHDFVVREMIGPLRADSSGRLH
                     WSGTVALQSHWKHPDLGIAAFVQDIRDGDVLQALQAPLCSKP"
     gene            3875..4324
                     /locus_tag="VE030_02220"
     CDS             3875..4324
                     /locus_tag="VE030_02220"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HZF22262.1"
                     /translation="MSISIRSPRDFCAGLIFLFFGALAAYIARDYPMGSAVRMGPGYF
                     PYLLGILLALLGVVICIKSLVAHGERIESASLRALLLVLGAIGVFAGTIESAGIVIAT
                     ALLVGIGAAASPESRLREVVVLIVFMLALAVGLFTYGLGLPFRILPI"
     gene            4324..5823
                     /locus_tag="VE030_02225"
     CDS             4324..5823
                     /locus_tag="VE030_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HZF22263.1"
                     /translation="MELLANLQLGFEIALTLQNILYCLVGVFLGTLIGVLPGIGPVAT
                     IAMLLPATFTLAPVSALIMLAGIYYGAQYGGSTTAILVNLPGEASSVVTCLDGYAMAR
                     QGRAGPALAIAALGSFFAGTVSTLILAMFAPPLAEIAFKFGPAEYFSLMVLGLIAAIV
                     LAHGSLINAIAMILLGLLLGLVGADTNTGQFRFTFGLPQLTDGIGFVVVAMGLFGFSE
                     IIANLERRAKREVVMAKITSLFPTRDDLKASWKAVLRGTGIGALLGILPGGGATLGSF
                     AAYALEKKVAKDPTRFGKGAIEGVAGPESANNAGAQTSFIPLLTLGIPTNAVMALMIG
                     AMTIHGITPGPQVMTERPELFWGMVASMWIGNLLLVVLNLPLIGIWVKLLSVPYRILY
                     PAILLFSCIGVYSLNNSAVDVALALAFGFLGYLFIKFDCEPAPLLLGFVLGPMMEENL
                     RRALLLSRGDPTVFVTRPLSLALLVAAAALLVIVVVPAVRRKRAEAFQE"
     gene            5827..>6791
                     /locus_tag="VE030_02230"
     CDS             5827..>6791
                     /locus_tag="VE030_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZF22264.1"
                     /translation="MAEFFRDLAGPSRRTWLHALIFVFGACAASATLPQDYPSRPIRI
                     IVPFPGGGTADLLARQIAQTMSEALRQQVVIENRTGAGGNIGADLAAKSKPDGYTLLM
                     GTVSTHAINPNLYAKMPYDPVKDFAPVTMVARMPNLLVVHPSVPAKNVAELIALAKAR
                     PGALAFASAGNGTTQHLAGELFKKMAGVDMIHVPYKGNAPAVTDLVGGQVQVMFDNIP
                     VSLQQVRAGKLRALAVTGPVRSPVLQDLPTVAEAGLPGYSITSWFGLYAPSGTSPQII
                     ARLNREANRALATAQIRRRLTDQGIEPTGGTPGHFADFMHAELVK"
BASE COUNT         1222 a         2124 c         2115 g         1330 t
ORIGIN      
        1 cgaagagacg ctcacggtcg cccatcgaca gattggcggc cccttccagc aacgccttgg
       61 ccgccgccag cgcttcttcg tccccctgct cctggtagcg gttgcgcaat tcccggtaga
      121 gctttccgcg cttgaccccg agctttttga tcgcggcttc ggctttctgc tcgaatccgg
      181 actcgcggcc gtagacgtga ttgaaacggc gcgcgatttc gcgggtgaac tcgatgtgcg
      241 gaacctggtc ttcgcccact ggcacacgat tggcgcggta gatcaggatg tcggcgcttt
      301 gcagcagcgg atagccgagg aagccatagg tggcaagatc cttctcggtc agcttctcct
      361 gttggtcctt gtaagagggg acgcgctcga gccagccgag cggcgtgatc atcgacagca
      421 gcagatgcag ttccgcgtgt tccggcaccc gcgactggat aaacagcgtc gcctgcgacg
      481 ggtccacccc ggccgcaagc cagtcgatca gcatgtccca gacgtgctgc tcgatcactt
      541 cgggcgtgtc gtaatgcgtc gtgagcgcat gccagtccgc cacgaagaac aggcattgga
      601 attcgtgctg cagcctcacc cagttcttca gcacgccgtg gtagtgaccg agatgcaggc
      661 tcccggtagg tcgcattcct gaaagcacgc ggtcgacaaa cattttttcc tctaacgaat
      721 tccgaaaatc cagccgatat cgaaaattgc cgccagtcct ccgatgaagg cgccgacgaa
      781 cggcatcatg acggtgccga gaacgcccag gaacatcagc accagcagga tgatcattcc
      841 atagggttcg accatcgcga aacgataggc tgcggcgcgc ggcagcaggc ttaccgcgat
      901 tcgcccgccg tcgagcggcg gcagcggcag gagattcaac accatgagca ccacgttgat
      961 gatgatgccg ccgcgcgcca tcagaataag aggctcggtg aaataattct gcggcatgac
     1021 ttgcgccagc ttgattgcca gcgcccacat gaccgccatc aacaggttcg cgcccgggcc
     1081 cgctgcggcg acccacagca tgtcgcgctt cggattgcgc aacctgccga agttcaccgg
     1141 cacaggcctg gcccagccga acgcatactt gccgccgctc gcgatcagga tcagcgccgg
     1201 tatcaatatg gtgccgatcg ggtcgatgtg acggatcggg ttgagtgtga tccggcccgc
     1261 ctcgtacgcg gtcagatcgc cgaagtgcct ggccgcgtag ccgtgcgccg cctcgtgcag
     1321 cgtgatcgcg aaaatccccg gtatcgcgta caacgcaatg agacgaacaa tgctttcgaa
     1381 ttcggtcatg cgtttacccc gaacggtgcc gcggtcccct tgccctgcct cacgatcacc
     1441 ggcgcctgct cggtgagatc caccaccgtt gtcgcttcca cgccgcaggg acctccgtca
     1501 aggatcagat ccacgtcatg ctcgaggcgg gcgcggattt cctcgccatc gttgagcggt
     1561 gtttcatcgc ccggaagaat cagcgtcgaa ctcaggatcg gttcgcgcat ctcctcgagc
     1621 agggcccgaa ccaccgcgtg atccggtaca cgcagtccga tagtcttgcg ccggggattg
     1681 agcaagcgtt tcggcacctc gcgcgtggcc ttgagaatga aggtatagct tcccggtgtc
     1741 gccgccttga gactgcggta ctgcgcgttg tcaacgactg catactgacc cagttccgac
     1801 agatcccggc ataccagggt gaaatgatga tgctcgtcta ccccgcggat gcgccggatg
     1861 cgctccatcg cggtcttgtc gccgatatga cagccgagcg catagcagga gtcggtggga
     1921 tacaccatca atccgccgcc gcgcacgatt tccactgccc gccggatcag gcgcggctgg
     1981 ggattattca cgtggatgga aaaatactgt gccatggctg gctcaggctg cggaccgcaa
     2041 cacttcccag tcctgccata acgtccgcgc gccatccgga aagggcggaa tatcgcccag
     2101 atcggccggt ccttgcgccg ccgcgtggaa gtcagagccg gcggataggc tgaatccgaa
     2161 gtgcgttgcc aagcgtaaca tcgggagagg attggcgcga cgcccgccgt agagttcaag
     2221 cgcctggccg ccatgctgtt tgaattcgtc gagcaacgcg tgcatttgca gcggcgcaag
     2281 acggtaccgg tccgggtggg ccagcacggc gtggccgccc gctccacgga tccatgcgat
     2341 tgcctgcccg agggagaccc actgatgcgc gacgtaaccg cacttaccca tccccaggta
     2401 ctttttgaat gcggtcttgg cgtcggcggc ctgcccctgc tccaccagaa agcgggcgaa
     2461 gtggccgcgg cctatgacgg ctgcgtctcc ggcgagccgg gaggcaccag ccagactgtt
     2521 cgggataccc gcggcgtcaa gctgctgcgc gatccgcacg gcacgaagcc cgcgcgacag
     2581 gcgaatcccg gccaggccat gggtaagcgg tgcgttgcgc gcctctatgc cgagtccgat
     2641 cacatgcacg gtgaggccgg cccaggtgac cgaaacttcg actcccggca cgaagccgat
     2701 gccggcctcg cgagccgctg ccgccgcctc cgccaacccg ccgacttcgt catggtcggt
     2761 gagcgcgagc atggtgaccc ggttgttaga cgcgcgctcg atcagcgccg cgggagtcaa
     2821 cactccatcg gaatacgtgg agtggctgtg taagtcgatg cgcaaggatt gcactgctgg
     2881 aggaataagc tatgcatcat aacaaatcgc ttcgcccgga aaaacctttc tccgccggag
     2941 gccgtacacg aacgagagcg ggaaacttga gaattcgcag gacgacggcg atgtgcgttg
     3001 ctgtcggctt ggtactgacg atgacttacc tgccgcttgc cggtgcagaa ccgggctgcg
     3061 aatcgcacag cacccccagg cgcgtggcgg tgattgagtt gtacacctcc gaaggctgca
     3121 acagttgccc gctcgcggac cggtgggtga gcagcctgcc gctacggggt ttcaacgcgg
     3181 accgtgtggt gccgctcgcg ttccatgtcg gttattggga ctatctcggc tggcgcgacc
     3241 gtttcgccca ggcaagcttc agtgtgcgcc agcgtgcgca agccgatcgc ggcagcgcgc
     3301 gcttcgtcta tacgccgcaa ctcttactca acggagcgga ctttcgtcag cctttccttg
     3361 actcggtatt ttcggaacgg ctggcgaaag taaacagcag ggtcgcagca gccgatttgc
     3421 gtctggtgca aagggcggat agtgccggtg tgagcattga actcgaaagc cgtgtgatcg
     3481 aaccctcggc gcataaattc tccgagacct atgttgcgtt gcttgagaac aacctcagca
     3541 gcgaagtgcg cgccggtgag aacagcggca aggtgctgct gcatgatttc gtggtgcggg
     3601 aaatgatcgg acctttgcgt gccgatagtt ccggccggct gcactggagc ggaaccgtgg
     3661 cgttgcagag ccactggaag catcccgatc tggggatcgc ggcattcgtg caggacatcc
     3721 gggacggcga cgtgctgcag gcgctgcagg ctccgctgtg cagcaaaccc tgacgcctgt
     3781 cttttttcgc tctgcggttt gctcacggag gaaagattgt gaaagaatag atctcacccc
     3841 gtctcgaaac accacaagaa cactcgggca ggagatgagc atcagcattc gcagtccccg
     3901 ggatttttgc gccggtctga ttttcctgtt ctttggcgcc ctcgcggctt acatcgcccg
     3961 cgactacccg atgggaagcg cagtacgcat gggtcccggc tatttcccct atctcctcgg
     4021 catcctgctc gcgctgctcg gcgtggtgat ctgcatcaag agcctggtgg cccacggcga
     4081 gcgcatcgag tccgcgagtc tgcgcgcgct gttgctggtg ctcggcgcaa tcggagtctt
     4141 tgccggcacg attgaaagcg ccggcattgt catcgccact gccctgctgg tggggatcgg
     4201 ggccgcagcc agccccgaga gccggctgcg ggaagtcgtg gtcctgatcg tgttcatgct
     4261 ggcgctggca gtgggcctct tcacctacgg ccttggcctg ccgttcagga tattgcccat
     4321 ctgatggagt tgctcgccaa tctgcaattg gggttcgaaa tcgcactgac gctgcagaac
     4381 atcctgtatt gcctcgtcgg cgtatttttg ggcaccctga tcggcgtatt gcccggcatc
     4441 ggcccggtgg caaccatcgc tatgctgctg cccgccacct tcacgctggc tccggtgtcg
     4501 gcgctcatca tgctcgccgg catctattac ggcgcgcagt acggcggttc gaccacggcg
     4561 atcctggtaa atcttcccgg ggaagcctct tcggtggtca cctgcctgga cggttacgca
     4621 atggcgcgac aaggccgcgc tggtcctgcg ctcgctatcg ccgcactcgg atcgttcttc
     4681 gccgggacgg tctcgacgct gatcctggcg atgtttgctc caccccttgc cgagattgcg
     4741 ttcaagttcg gcccggccga atatttttcc ctgatggtgc tgggtctgat tgccgcgatc
     4801 gtgctggccc acggttcgct catcaacgcc atagcgatga tcctgctggg cctgctgctg
     4861 ggattagtcg gcgccgacac caacaccggg cagttccgct tcaccttcgg cctgccccag
     4921 ctcaccgatg gcatcggctt cgtagtggtg gcgatgggtc tgttcggttt ttccgagatc
     4981 atcgccaacc tcgagcgccg cgccaagcgc gaagtggtca tggcgaagat caccagcctg
     5041 tttcccacgc gggatgacct caaagcatcg tggaaagcag tattgcgcgg caccggaatc
     5101 ggggctctcc tgggtatact gccgggcggc ggcgccacgc tgggatcgtt cgctgcatat
     5161 gcgctggaga agaaagtggc caaggatccg acgcgcttcg gaaaaggcgc gatcgagggc
     5221 gtggccgggc cggaatcggc gaacaacgcc ggcgcgcaga cctccttcat tccattgctg
     5281 acgctcggca tcccgacaaa tgcggtgatg gcgctgatga tcggcgcgat gaccattcac
     5341 ggcattaccc caggcccgca ggtgatgacc gaacgcccgg aactgttctg gggaatggtt
     5401 gccagcatgt ggatcggcaa cctgcttctg gtggtgctga atcttccgtt gatcggcatc
     5461 tgggtgaagc tgctgtcggt gccgtaccgg atcctctatc ccgctatact gttgttctcc
     5521 tgcatcggcg tgtacagcct caacaacagc gcggtcgacg tcgcactggc gctggccttc
     5581 ggcttcctcg ggtatctgtt catcaagttc gactgcgaac cggcgccgct gctgctcgga
     5641 ttcgtgctcg gcccgatgat ggaggaaaac ctgcgccggg cgctgctgct ctcccgaggg
     5701 gacccgaccg tgttcgtgac gcgcccgctg agcctggcgc tgctggtggc tgcggcggcg
     5761 ctgctggtca tcgtggtggt gccggcggtg cgcaggaagc gcgcggaagc gttccaggaa
     5821 taggccatgg cagagttctt cagggatctt gccggccctt cgcgacgcac ctggctgcat
     5881 gcgttgatat ttgtattcgg cgcatgcgcg gcaagcgcaa cgctgccgca ggactatccc
     5941 agccggccga tccgcatcat cgtaccgttt cccgggggcg gcaccgccga tctgctggcg
     6001 cgccagatcg cccagacgat gagcgaggca ttgcgccagc aagtcgtcat cgagaaccgc
     6061 accggcgccg gcggtaatat cggcgcggat ctcgcagcga agtcgaaacc tgacggctac
     6121 acgctgctca tgggtactgt ttccacccat gcgatcaatc cgaatctcta tgcgaagatg
     6181 ccttacgatc cggtgaagga tttcgcgcca gtaacgatgg tagcgaggat gccgaatctt
     6241 ctggtggtcc acccatcggt gccagcaaaa aatgtcgcag aattgatcgc gttggcgaaa
     6301 gccagacccg gcgcccttgc ctttgcgtcc gcaggcaacg gcaccactca gcatcttgca
     6361 ggcgagcttt tcaagaagat ggcgggcgtg gacatgatcc acgtgcctta caagggcaat
     6421 gccccggccg taaccgatct ggtcggcgga caggtgcagg tgatgttcga caacatcccg
     6481 gtgtcactcc aacaggtgcg cgccggcaag ctgcgcgcgc ttgcggtgac gggtcccgta
     6541 cgttcgccgg tgctgcagga tttgcctacc gttgccgaag ccggactgcc cggctacagc
     6601 atcacctcgt ggttcggcct ctatgcgccg tcgggcacct cgccgcagat catcgcgcgc
     6661 ctgaaccggg aggccaacag ggcgctggca actgcgcaga tccgccggcg attgactgac
     6721 cagggaatcg aacccacggg cggtacaccg ggccatttcg cggacttcat gcatgccgaa
     6781 ctggtgaaat g
//
LOCUS       DASQOP010000060         7411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1635456, whole genome shotgun sequence.
ACCESSION   DASQOP010000060 DASQOP010000000
VERSION     DASQOP010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7411
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1635456"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VE030_02235"
     CDS             complement(<1..229)
                     /locus_tag="VE030_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22265.1"
                     /translation="MSVQTLYLLVLLVPLVPLAGALIAGLLGWLIGRRATHWVAIIGM
                     TVSTVAAFLVFRDVLAGNAFNGPVYTWITTGD"
     gene            complement(341..646)
                     /gene="nuoK"
                     /locus_tag="VE030_02240"
     CDS             complement(341..646)
                     /gene="nuoK"
                     /locus_tag="VE030_02240"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003261941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HZF22266.1"
                     /translation="MISLSHYLVLGAVLFAISVVGIFLNRKNVIILLMAIELMLLAVN
                     INFVAFSHFLEDTAGQVFVFFILTVAAAESAIGLAILVVLFRSVHTINVDDLDKLKG"
     gene            complement(776..1387)
                     /locus_tag="VE030_02245"
     CDS             complement(776..1387)
                     /locus_tag="VE030_02245"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HZF22267.1"
                     /translation="MNFQSFIFYFFAAMLVFAALRVITARNPVHSALFLVLAFFTAAA
                     LWMLLEAEFLAIALVLVYVGAVMVLFLFVVMMLDINLDRLREGYWDYLVPALIVAGVM
                     VAEMAIILGARYFDLEGMPAPEPGGGDYSNTRELGLLIYTDYVYPFEIAAVILLVAIV
                     AAIALTLRHRKDTKYIDPAKQVNVRRKDRVRMVSMPSEGKDEG"
     gene            complement(1579..2067)
                     /gene="nuoI"
                     /locus_tag="VE030_02250"
     CDS             complement(1579..2067)
                     /gene="nuoI"
                     /locus_tag="VE030_02250"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HZF22268.1"
                     /translation="MERIRDFFRTFLLFELVKGLMLTGRYFFARKITVQFPEEKTPQS
                     GRFRGLHALRRYPNGEERCIACKLCEAVCPALAITIESEQRADGTRRTTRYDIDLTKC
                     IFCGFCEESCPVDSIVETRILEYHGEKRGDLLYTKEMLLAIGDRYEEQIAKDRADDAA
                     YR"
     gene            complement(2081..3106)
                     /gene="nuoH"
                     /locus_tag="VE030_02255"
     CDS             complement(2081..3106)
                     /gene="nuoH"
                     /locus_tag="VE030_02255"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HZF22269.1"
                     /translation="MSFAFLEDLIGPGGVTALWTLAKILAIVIPLILSVAYLTLAERK
                     VIGYMQIRIGPNRVGYKGLLQPFADVLKLLVKEVIVPSGANKFLFMLAPVLAIMPALA
                     AWAVVPFSPELVLADVNAGLLYVMAITSMGVYGVIIAGWASNSKYAFLGAMRSAAQIV
                     SYEIAMGFALVCVLMMSGSLNLSTIVNEQKSDWGLLGWNLIPLLPMFLVYFISGVAET
                     NRAPFDVAEGESEIVAGFHVEYSGTAFAVFFLAEYANMILVSAMASIMFLGGWLSPVP
                     FVPDGFPWLAIKMALLLFCFLWFRATFPRYRYDQIMRLGWKVFIPLTIVWLVLVGGLM
                     QTRYWPW"
     gene            complement(3103..5487)
                     /gene="nuoG"
                     /locus_tag="VE030_02260"
     CDS             complement(3103..5487)
                     /gene="nuoG"
                     /locus_tag="VE030_02260"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HZF22270.1"
                     /translation="MAHLEIDGRPVEVPDNSTIMDAANKLGIYVPHFCYHKKLSIAAN
                     CRMCLVQVKKAPKPLPACATPATEGMTVYTHSEAAIKAQKGVMEFLLINHPLDCPICD
                     QGGECQLQDLAVGYGASSSRYTEPKRVVTNKELGPLVCADEMTRCIHCTRCVRFGQEI
                     AGIMELGMVGRGEHAEIMPFVARTVDSELSGNMIDVCPVGALTSKPFRYKARTWELAR
                     RKSISPHDGLGSNLVVQVKDNRVMRVLPLENEDVNECWLSDRDRFSYEALNSQERLTA
                     PMVKRDGTWEEVDWTTALEFVAAGLKRIRDGHGAKAVGTLASPQATVEELYLLGKFAR
                     GIGSGNVDFRLRQSDFSGDGSLRGAPWLGMKVAAVNGLDRVLLVGSTLRKDHPLLAQR
                     FRQAVKKGAQFNLINPVDDDLLMRVANKSIVAPSAMPEVLGQVLKAAAEAKAIAVPMV
                     VAGLEVSDSARVIAESLVSGAKRAVFLGNLAQHHPQAYQLHRLAQELSQIAGATFGFL
                     GEAANSVGGYLAGALPFGREPSGLNAAQMLIQPRRAYLLLNAEVELDTFDPRTARAAM
                     HGAELVVVMSPFKQRAAEYAQVLLPVSPFTETGGTYVNTEGLVQSFQGVVRPLGETRP
                     AWKVLRVLGTLLDIPGFEFDSIEQVRGEWQASVGDVASRLSNEIGGAPAVIARAECVA
                     IGRAEFERIGEVPIYHADAIVRRAESLQQTRDAQVGTAWLPGSLIDRLGLRQGDRLKI
                     IQAGGEATLSFGRDDRLSADCVRLPSGCRETAGLGAMLGLVSLERVAAQQRVTA"
     gene            complement(5498..6799)
                     /gene="nuoF"
                     /locus_tag="VE030_02265"
     CDS             complement(5498..6799)
                     /gene="nuoF"
                     /locus_tag="VE030_02265"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HZF22271.1"
                     /translation="MNAPLPPFPPDIYGPEAILLKGLTGLNWRLKDYEARGGYAALKK
                     ILRESIAPEQVIAEVKKSALRGRGGAGFPTGLKWSFMPRAFPGQKYLVCNSDEGEPGT
                     FKDREILRYNPHAVVEGMAIAAYAMGITVGYNYIHGEIWDVYERFEDALAEAYDAGYL
                     GKSIQGSAFSFDLYAHHGWGAYICGEETALLESLEGKKALPRFKPPFPASYGLYGKPT
                     TINNTETFAAAPWIIANGADAFLSLGRPNNGGTKIFSVTGHVERPGNYEVRLGTPFPK
                     LLAMAGGMRGGRKLKAVIPGGSSMPVLPAEVMMQTDMDYDAIAKAGSMLGSGAVIVMD
                     ETTCMVRALQRLSYFYFEESCGQCTPCREGTGWLYRMVHRIEHGQGRTEDLETLNSVA
                     DNIAGRTICALGDAAALPVKSFIKHFREEFQYHIDHKQCLV"
     gene            complement(6796..7272)
                     /gene="nuoE"
                     /locus_tag="VE030_02270"
     CDS             complement(6796..7272)
                     /gene="nuoE"
                     /locus_tag="VE030_02270"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HZF22272.1"
                     /translation="MLSPDSLAKIDKAVVKYPPNQRQSAMMAALTVAQDEKGWLSTET
                     MDFVAQYLGMPPIAVYEAATFYGMYNLQPVGRNKITICTNLPCALSGAADAAEHLKKR
                     LGIGFNETTPDGGFTLKEGECLGACGDAPVCLHNDKKMLSFMTPDKLDTLIEELRK"
BASE COUNT         1440 a         2358 c         2265 g         1348 t
ORIGIN      
        1 tgtcgccggt ggtgatccag gtataaaccg ggccgttgaa tgcattgccg gcgagcacgt
       61 cgcgaaacac cagaaaggcc gcaaccgtcg acaccgtcat tccgatgatc gccacccagt
      121 gcgtggcgcg ccggccgatc aaccagccca gcaggccggc gatcagcgcg ccggcaagag
      181 gcactagggg gacgagaagg acgagaagat agagggtctg gacgctcatt atcattcgct
      241 gagtgctgag tgctgagtgc tgagcgctga gaggttcccc ccagaccgag cctcggttcc
      301 gttggttcgc agcacgcagc acgcagcacg cagcactttt ctatcctttt agtttgtcca
      361 gatcatcgac attgatggtg tgtacgctcc ggaacagcac caccaggatc gccagaccga
      421 ttgccgattc ggccgccgcc acggtaagga tgaaaaacac gaatacctgg ccggcggtgt
      481 cctcaaggaa atgcgaaaaa gcgacgaaat tgatgttcac cgccagcagc atcagctcga
      541 tcgccatcag caggatgatc acgttcttgc gatttaaaaa gatgcccacc acgctgatcg
      601 cgaacagcac ggcgccgaga acgaggtagt gggagaggga gatcatggcc gccctcccgg
      661 cgccgaaagg atgaaggatg aaggatgaag gatgaagtga aacccgaaac agcggctcgt
      721 cctagcaact ccgctgcgcc cggtcaccgt gcttttcacc ttccgccttc cgccttcagc
      781 cttcatcctt gccctccgac ggcatcgaaa ccatgcgcac gcggtccttg cggcgcacat
      841 tgacctgctt cgccgggtcg atgtacttgg tgtccttgcg atgacgcagc gtcagtgcaa
      901 tggcggcgac gatcgccacc agcaggatta ccgcggcgat ctcgaacgga tagacgtagt
      961 cggtatagat caggaggcca agctcgcgcg tgttgctgta gtccccgccc ccgggttcgg
     1021 gcgccggcat gccttcgagg tcgaagtagc gggcgccgag gatgatcgcc atctccgcca
     1081 ccatcacgcc ggccacgatc agcgccggca ccaggtaatc ccagtagccc tcgcgcaggc
     1141 ggtcgaggtt gatgtcgagc atcatcacca cgaacaggaa cagcaccatc accgcgccga
     1201 cgtagaccag caccagcgca atggcgagga actcggcctc gagcagcatc cacagcgccg
     1261 cggcggtaaa aaacgcgagc accaggaaaa gggccgagtg caccggattg cgcgcagtaa
     1321 tgacgcgcag cgcggcgaac accagcattg ccgcgaagaa atagaaaatg aagctctgaa
     1381 aattcactat aaaaaactat aaggtcttaa ccacagagac acagaggcac ggagaggaac
     1441 aaaggcaaga agggttcctc attgattgca ttaaagccag attcttcatt ctttcttgtt
     1501 cttcacgcgt tgctgaatgg attctcttgt tcttgaactc ttctctgtgc ctctgtgtct
     1561 ctgtggtgag atccaccgtt aacggtatgc ggcgtcgtcg gcgcggtcct tggcgatctg
     1621 ttcctcgtag cggtcgccga tcgccagcag catctccttg gtgtacagga ggtctccgcg
     1681 tttttccccg tggtattcga ggatgcgggt ctcaacgatc gagtccaccg gacaggactc
     1741 ctcgcagaaa ccgcagaaga tgcacttggt gaggtcgatg tcgtagcgcg tcgtgcgccg
     1801 ggtaccgtcg gcacgctgct cggactcgat ggtgatcgcc agcgccgggc acaccgcttc
     1861 acacagcttg caggcgatgc agcgttcctc gccgttcgga tagcggcgca gggcgtgcaa
     1921 gccgcggaag cgccccgatt ggggggtttt ttcctccgga aactgcaccg tgatcttgcg
     1981 cgcgaagaaa tagcggccgg tgagcatcag gcctttcacc agttcgaaca gcaggaaggt
     2041 acggaagaag tcgcggatgc gctcgatcat ttccccgccc tcaccacggc cagtaccgcg
     2101 tctgcatcag tccgccgacc agaaccagcc agactatcgt caacgggatg aaaaccttcc
     2161 agcccagccg catgatctgg tcgtagcggt agcgcggaaa agtggcgcgg aaccacagaa
     2221 aacagaacag cagcaaagcc atcttgatgg ccagccacgg gaaaccgtcc ggaacgaatg
     2281 gaaccggcga gagccagccg ccgaggaaca tgatcgacgc catggcagaa accaggatca
     2341 tgttcgcgta ctcggccagg aaaaacaccg caaacgcggt gcccgagtat tcgacgtgga
     2401 aaccggccac gatctcggat tcgccctccg ccacgtcgaa aggcgcgcgg ttagtctccg
     2461 caacgccgga aatgaaatac accaggaaca tcggcaacag cgggatcagg ttccagccca
     2521 gcagccccca gtcggacttc tgctcgttga cgatggtaga gaggttcagg ctgcccgaca
     2581 tcatcagcac gcacaccagg gcgaagccca tcgcaatctc gtaggaaacg atctgcgccg
     2641 cagagcgcat cgcgccgagg aacgcgtact tggagttcga cgcccagccg gcaatgatta
     2701 cgccgtacac gcccatcgag gtgatcgcca tgacgtaaag cagcccggcg ttgacatccg
     2761 ccagcaccag ctccggagaa aacggcacca ccgcccaggc cgccaacgcc ggcatgatcg
     2821 cgagcaccgg ggccagcatg aacaggaatt tattcgcgcc ggagggcacg atgacttcct
     2881 tgaccagcag cttgagcaca tcggcgaacg gctgcagcag gcccttgtag cctacgcggt
     2941 tcgggccaat gcggatctgc atgtagccga tgaccttgcg ctccgccagc gtaaggtagg
     3001 cgacgctcaa tatgagcggg atgacgatgg cgagaatttt cgccagcgtc catagcgcgg
     3061 tgacaccgcc gggcccgatg agatcctcga ggaaggcgaa actcatgcgg tcactctctg
     3121 ctgcgcggcg acgcgttcca ggcttaccag gccgagcatc gctcccagac ctgcggtctc
     3181 gcggcagccc gagggcaggc gcacgcagtc cgccgatagg cggtcatcgc gcccaaagga
     3241 cagcgtggct tcgccgcccg cctggatgat cttgaggcgg tcgccctggc gcagcccaag
     3301 cctgtcgatc aggctcccgg gcagccaggc cgtccccacc tgcgcgtccc gggtttgctg
     3361 cagcgattcc gcgcgccgca cgatcgcgtc cgcatggtag atcggcactt cgccgattct
     3421 ctcgaactcg gctctcccga tggcgacgca ctcggcccga gcgatcaccg ctggagcacc
     3481 gccgatttca ttcgacagcc tggacgcaac gtcgccgacc gaagcctgcc actcgccgcg
     3541 cacctgctcg atgctgtcga actcgaaacc cggaatgtcg agcagagtac cgagcacgcg
     3601 caacactttc cacgccgggc gcgtttcgcc cagcggccgc accactccct gaaagctctg
     3661 caccagcccc tcggtattga cgtaagtgcc gccagtctcg gtgaacggcg aaaccggcag
     3721 cagaacctgc gcatactcgg cggcgcgttg cttgaacggg gacatgacca ccaccagttc
     3781 ggcgccgtgc atcgccgcgc gcgcggtgcg cggatcgaag gtatccagtt cgacttccgc
     3841 gttcagcagc aggtaagccc ggcgcggctg gatgagcatc tgcgcggcgt tcaggcccga
     3901 aggttcgcgc ccgaacggca gcgctccggc gagataaccg ccgacgctgt ttgccgcctc
     3961 gcccagaaaa ccgaaggtcg cgccggcgat ttgcgaaagt tcctgcgcca ggcgatgcaa
     4021 ttggtaagcc tgcggatgat gttgcgcgag gttgccgaga aatacggcgc gttttgcgcc
     4081 ggaaaccagg ctttcagcga tcacgcgcgc cgaatcgctt acttcgaggc cggccaccac
     4141 catcggcacg gcaattgcct tggcctcagc ggcagccttc agcacctggc cgagcacctc
     4201 gggcatcgca ctcggggcga cgatcgactt gttggccaca cgcatcagca gatcgtcgtc
     4261 caccgggttg atcaggttga actgcgcccc tttctttacc gcctggcgga accgctgcgc
     4321 caacagggga tggtccttgc gcagagtcga gcccaccagc agcacccggt ccaggccatt
     4381 gaccgcggcg accttcattc ccagccaggg tgcgccgcgc aggctcccgt ctccggaaaa
     4441 gtccgattga cgcaaacgga aatcgacatt gccgctgccg atgccgcgcg caaacttgcc
     4501 gagtagataa agctcttcca cggtcgcctg cggcgaagcg agcgtgccga ccgcctttgc
     4561 gccatgccca tcacggatgc gcttgagtcc ggcggccacg aactcgagcg cggtagtcca
     4621 gtccacctcc tcccaggtgc cgtcgcgctt gaccatcggc gcggtcagcc gttcttgcga
     4681 gttgagcgcc tcgtaggaga accggtcccg gtcggagagc cagcactcgt taacgtcttc
     4741 gttctcgagc ggcagcacgc gcatgacgcg gttgtccttt acctggacca ccagattgga
     4801 gccgagtccg tcgtgcgggc tgatggattt gcgccgcgcc agctcccagg tgcgcgcttt
     4861 gtagcggaac ggtttggaag tgagcgcgcc caccggacag acgtcgatca tgttccccga
     4921 aagctcggaa tccacggtgc gcgccacgaa cggcatgatc tcggcgtgct cgccgcggcc
     4981 caccatgccg agttccatga tgccggcaat ctcctggccg aagcgcacgc agcgggtgca
     5041 gtgtatgcag cgggtcatct cgtcggcgca gaccagcggc ccgagttcct tgttcgtgac
     5101 cacgcgcttg ggttccgtat agcgggaact cgacgccccg tagccgaccg cgaggtcctg
     5161 cagctggcac tcgccgccct ggtcgcaaat cgggcagtcg agcggatggt tgatgagcag
     5221 gaattccatc acgcctttct gcgccttgat cgcggcctcc gagtgggtat agaccgtcat
     5281 tccctcggtg gccggggtgg cgcaggcagg cagcggcttc ggcgcttttt tcacctgcac
     5341 caggcacatg cggcagttgg cggcgatcga cagtttcttg tgataacaga aatgcggaac
     5401 gtagataccg agcttgttgg cggcatccat gatggtgctg ttgtccggta cttcgaccgg
     5461 ccggccgtct atttcgaggt gtgccatcag tctgatctca taccaggcac tgcttgtggt
     5521 cgatgtggta ctggaactct tcgcggaaat gcttgatgaa gctcttcacc ggcagcgccg
     5581 cggcgtcgcc cagcgcgcag atggtgcggc cggcgatgtt gtcggccacc gagttcagcg
     5641 tttccagatc ctcggtccgg ccctgcccat gctcgatgcg atggaccatc cggtacagcc
     5701 agccggtacc ttctcggcag ggcgtgcact ggccgcagga ttcctcgaaa tagaaatacg
     5761 acaggcgctg cagcgcgcgc accatgcaag tggtctcgtc catgactatg accgccccgg
     5821 agcccagcat agaaccggcc ttggcgatcg catcgtagtc catgtcggtc tgcatcatga
     5881 cttcggcggg cagcaccggc attgaagaac ctccgggaat gaccgctttc agcttgcgcc
     5941 cgccgcgcat acctccggcc atcgccagca atttcgggaa gggcgtgccg agccggactt
     6001 cgtagttgcc gggacgctcg acgtgaccgg ttaccgaaaa gatcttggtg ccgccgttgt
     6061 tgggccggcc gaggctcagg aaggcgtcgg cgccattggc gatgatccag ggtgccgcgg
     6121 caaacgtctc ggtgttgttg atcgtggtcg gcttgccgta caggccgtag ctcgccggga
     6181 acggcggctt gaagcgcgga agcgccttct tgccttcgag cgactccagc agcgcggttt
     6241 cctcgccgca gatatacgcg ccccagccgt ggtgtgcgta cagatcgaag ctgaatgccg
     6301 agccctggat gctcttgccc agatatccgg cgtcgtaagc ttcggcgagc gcgtcttcga
     6361 agcgctcgta cacgtcccag atctcgccgt ggatatagtt gtagccgaca gtgattccca
     6421 tcgcgtaggc cgcgatcgcc atgccttcga cgacggcgtg cgggttgtag cgcaggatct
     6481 cgcggtcctt gaatgtgccg ggttcgccct catcggagtt gcacaccagg tacttctggc
     6541 cggggaatgc acgcggcatg aagctccact tcaatcccgt gggaaagccc gcgccgccgc
     6601 ggccgcgcaa ggctgatttc ttcacttcgg cgatcacctg ctccggcgcg atgctctcgc
     6661 gcaggatctt cttcagcgcc gcgtagccgc cgcgcgcctc gtagtccttc agccgccagt
     6721 tgagcccggt gagccccttg agcaggattg cctccgggcc gtagatatcc ggcgggaaag
     6781 gcgggagagg ggcattcatt tcctcagctc ctcgatcagc gtatccagtt tgtccggcgt
     6841 catgaacgac agcattttct tgtcgttgtg caggcacacc ggcgcgtcgc cgcaggcccc
     6901 caggcattcg ccttccttga gggtgaaacc gccgtccggg gtggtttcgt tgaagccgat
     6961 gccgagtctc ttcttcaggt gctcggccgc atcggcggcg cccgacaggg cacacggcag
     7021 gttggtgcaa atggtgatct tgttgcgccc gaccggctgc aggttgtaca tgccgtagaa
     7081 agtcgccgct tcatacaccg cgatcggcgg catgcccagg tattgcgcga cgaaatccat
     7141 ggtctcggtg gagagccagc ccttctcgtc ctgcgcgacg gtgagcgcgg ccatcatcgc
     7201 cgactgtcgt tggttgggtg gatacttgac aaccgccttg tcgatcttcg ccagcgaatc
     7261 cggcgacaga atcatcggcg cgttcatctg tccacttccc cgaaaacgat gtcctgggtg
     7321 ccgatgatgg cgaccacgtc ggcgatcatg tggccgcgtg ccatttcatc gagcgccgcc
     7381 agatgggcaa aacccggtgc gcggattttc a
//
LOCUS       DASQOP010000061         5791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1434465, whole genome shotgun sequence.
ACCESSION   DASQOP010000061 DASQOP010000000
VERSION     DASQOP010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5791
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434465"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="VE030_02275"
     CDS             <1..885
                     /locus_tag="VE030_02275"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HZF22273.1"
                     /translation="TGKEIVARAIHAASPRHAQAFVPINCGAIPEALLESQLFGHVRG
                     AFTTAVQASPGLFAAADRGTIYLDEIGDLPLHMQVKLLRVIEEKIVWAVGSTKPVLVD
                     ARIIASTNRDLVKEIEGGRFREDLFYRLNVVHIALPPLRERREDMGLLVEHFVRALNL
                     KLSTKFLGVDREVLSVLMNHPWKGNVRELEHVLESAMILGEGDVISLPDLPDYLVTAT
                     RASASSDALRDVTRRFERQHIMTVLTQTQFDKKEAARRLGISLASLYRKIGEHAIQAN
                     DTETPTERALLTLAKPRL"
     gene            1184..2263
                     /locus_tag="VE030_02280"
     CDS             1184..2263
                     /locus_tag="VE030_02280"
                     /inference="COORDINATES: protein motif:HMM:NF040603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor P family protein"
                     /protein_id="HZF22274.1"
                     /translation="MNQQMRQFVASLAAMGLIGLLVGLTTGTAGAWNFAPANSVSGEA
                     FGVSVKASVNAAEVTVGPTPYVVLPPDGGMVSERVLRIAVPNVAASSTLEVVTTGSIG
                     PATATAQSSATVERANLLNGLVTAQLVVAMSSSTADGSTAGSTAEGSTLIGLSINGSV
                     PVDVTPPPNTTIPIPGGAVILNEQYLEGDGVHTRALTVNMIHVMLRDPVTGAITVDII
                     VASAHSDVNFVPAPKAGNAFMTGGGKLGTGRDIATFGFNAGSRAGGGLHGQLQYNDHA
                     QNLKVHSLSIDSFKLITGTTCVTFSGGARVNNVDGYRFTVNQACDNGEPGVGNDTFDI
                     SVSGPGIWYSRRGTLTGGNLQLHPE"
     gene            complement(2542..3927)
                     /locus_tag="VE030_02285"
     CDS             complement(2542..3927)
                     /locus_tag="VE030_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HZF22275.1"
                     /translation="MSLPATALQSAIDELKALFGERLSTSTGVRDHHSKDESWHHPHR
                     PDAVVFPNTTEEVAEIVRICARHKTPVIAYGTGTSLEGNVIPHQGGVVVDLANMNQVL
                     RVNPEDLDVTVQARVTRKELNAHIRDQGLFFPIDPGADASLGGMAATRASGTNAVRYG
                     TMRENVLALTVVLADGRIIRTSRRARKSAAGYDLTRLFVGSEGTLGIITEVTLRLYGI
                     PEAMAAAVCSFESIEGAVNTVIQTIQLGIPVARIELLDDVQMDSVNKFSKLNYPVKDT
                     LFIEFHGTQAGVKEQAELVQSIAAEHGGGEFKWATKPEERSHLWTARHDVTYANKALR
                     PGCEIWATDVCVPISRLAECILETKKDLKKSFLTAPLVGHVGDGNFHLGFLINRDDPK
                     ELAEAERLNERLVMRALAMDGTCTGEHGIGLGKMKFLTAEHGEAVAVMRQVKRALDPQ
                     NIMNPGKIFTL"
     gene            4078..5487
                     /locus_tag="VE030_02290"
     CDS             4078..5487
                     /locus_tag="VE030_02290"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HZF22276.1"
                     /translation="MRVVSVGAAAIALVSGLAVPAQLGEIPVAALSAGSFTVAVADST
                     AFSEPAPVLDYRQREKFLRGRHHFNQKWVVFPSLGGDWGLGPTFIADRCSGCHVGGGR
                     GDPPKSADQQLLSVLVRISIPGEDEHGGPKPHPNYGDQIQNQGLMGQDRNATYLGERV
                     PPEASLYLDWQEVPGTFADGERVMLRKPRLRVEKLNFGPLGPEVMYSLRIAQPTFGLG
                     LLEAVSETDILALAERQKSQGVNGRPNYVWDAIGKRLALGRFGWKSNQPSIRQQIAAA
                     LHGDIGVTSSLFDKENCPPVQEDCAYQPPGNQPELVDVNWDELEFWTQALAVPARRNV
                     VEPVFARGERAFNEAKCSVCHVPEMKAGVLPGLPQIQGQVFHAYTDLLLHDMGEALAD
                     GRPDFKAGPRDWRTPPLWGLGLSQIVSGSTAMLHDGRARNAAEAILWHGGEAEGAREA
                     FRNMPKADREALLKFLSSI"
     gene            5505..>5791
                     /locus_tag="VE030_02295"
     CDS             5505..>5791
                     /locus_tag="VE030_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22277.1"
                     /translation="MRPIICCWRTQGGRAWVAILAFGALACLPFEADAGTPEELLARY
                     EGEARTEAPAFHGFSVEEGRMFYFQKYPVPILGEVGCVSCHLQDPRRSVMR"
BASE COUNT         1070 a         1762 c         1896 g         1063 t
ORIGIN      
        1 accggcaagg agatcgtcgc acgcgccatc cacgcggcca gcccgcgaca tgcgcaggcg
       61 ttcgttccca tcaactgcgg ggcgattccg gaggcgctgc tcgagagtca gttgttcgga
      121 cacgtgcggg gggccttcac caccgcggtg caggccagtc ctggactatt cgctgcggcc
      181 gatcgcggca ccatctacct ggacgaaatc ggggacctgc cgctccacat gcaggtgaag
      241 ctcctgcggg tgatcgaaga gaaaatagta tgggccgtgg gcagcaccaa gccagtactg
      301 gtcgacgcgc ggatcatcgc cagcaccaat cgggatctcg tcaaggagat cgagggcggg
      361 cggttccgcg aagacctctt ctaccgcctc aacgtggtcc acatcgcctt gcctccgctg
      421 cgggaacggc gcgaggacat ggggctcctg gtggagcact tcgtccgcgc gcttaacctg
      481 aagctgtcga cgaagttcct gggtgtagac cgggaggtgc tcagcgtcct gatgaaccat
      541 ccctggaagg gcaatgtccg cgagctggag cacgtgctcg aaagcgcgat gatcctgggc
      601 gagggggatg tgatctcgct ccccgatctc ccggactacc tggtgacggc gacccgagcg
      661 tcggccagca gcgacgcgct ccgggacgtg acccgtcgct tcgagcgcca gcacatcatg
      721 accgttctga cgcagaccca gttcgacaag aaggaggcgg cccgccggct gggaatcagc
      781 ctggcctctc tctatcgcaa gatcggcgag cacgcgatac aggcgaacga cactgagact
      841 cccacagagc gggcgcttct cactttagcg aaaccgcgcc tataaagttc tcagaaacga
      901 gagcggcaag aaaatcaacg cccctcgtcg tttaactaca gggcgtcgat tttcaaccac
      961 cagtagcaat cacttggttg ggaacgtccc cctcaaaaga ccgcccagtt gaataacaca
     1021 aatgcggttt accctcgtct atcgcgcatc caatcgccgg atttcattga tttagtcttg
     1081 ttggcacggg cgttgcgacc atctcctgcg cgacttcgcc actgcgtttg ccgaggaggg
     1141 ggtcgccgaa taaaccaact acgactagga aaggagacgg tcaatgaatc agcaaatgcg
     1201 gcaattcgta gcgagcctcg cagcgatggg gttgataggg ttgttggtgg gcttaacgac
     1261 gggaaccgcc ggggcgtgga acttcgcccc ggcaaacagc gtcagcggcg aggcgttcgg
     1321 ggtctcggtc aaggcgtcgg tcaatgccgc cgaggtcacg gtggggccaa ccccctacgt
     1381 ggtgcttccg cccgacggcg gaatggtcag cgaacgggtg ctgcgcatcg ctgtgcccaa
     1441 tgtcgcggca tcgagcaccc tggaggtcgt caccacggga tcgatcgggc ccgccaccgc
     1501 caccgcccag agcagcgcga cggtggagcg ggcgaatctc ctcaacggtc tcgtcacagc
     1561 ccagctcgtg gtcgcgatgt cgagcagcac ggctgacggg tccacggcag ggagcacggc
     1621 cgagggttcg accctcatcg gtctgagcat caacggaagc gtgccggttg acgtgacgcc
     1681 cccgccgaac acaacgatcc cgatcccggg cggcgccgtg atcctgaacg agcagtacct
     1741 cgagggggac ggcgtgcata cgagagccct caccgtgaac atgatccacg tcatgctcag
     1801 agacccggtg acgggggcga tcacagtgga catcatcgtc gcctcggcac acagcgacgt
     1861 gaacttcgtc ccggccccca aggcgggcaa cgcgttcatg accggcggcg ggaagctggg
     1921 gaccggccgc gatatcgcca ccttcgggtt caacgcgggc tccagggcag gcggggggct
     1981 ccacggccag ctccaataca atgaccacgc ccaaaacctg aaagtgcaca gcctgtcgat
     2041 cgactcgttc aagctgatca ccgggaccac ctgcgtaacc ttttccggcg gcgcacgcgt
     2101 gaacaacgtc gacgggtaca ggtttacggt caatcaggcc tgcgataacg gcgagccggg
     2161 ggtcggaaac gacacgttcg acatctccgt ctcgggaccc ggaatctggt attcgcggcg
     2221 gggaacgttg acgggcggaa acctgcagct tcatcctgaa tagccggcgg gagcatgccg
     2281 gagaccggac gggagcggcc ctagccgccc ccgtcgcacg ttgtggtgcg aaacgcaaaa
     2341 gcgtgaaggg gtgaagggtt taaggcgtga atgagtgaat gtgtgaaggg tgaagggtaa
     2401 gagctctagg gcattcgcca cgccgaaaac attgccgttc cgattttacc ccttcactca
     2461 ttcacccttc acaccttcac gcttttgagc atcgcaccct tcactcgttc acgctgttac
     2521 gtatcgcacc cttcacgatt tttacaaagt aaaaatcttg cccggattca tgatgttctg
     2581 cgggtcgagc gccctcttga cctggcgcat caccgcaact gcttcgccgt gttcggcagt
     2641 gaggaacttc atcttgccca gcccgatgcc gtgctcgccg gtgcaggtac catccatcgc
     2701 cagcgcgcgc atcaccagcc gttcgttcag gcgctcggct tcggcgagtt ctttcgggtc
     2761 gtcgcgattt atcaggaagc cgaggtggaa gttgccgtcg ccgacgtggc cgaccagcgg
     2821 cgcggtcagg aagcttttct tgagatcctt cttggtctcg aggatgcatt cggcgagacg
     2881 ggagatcggc acgcacacgt cggtcgccca gatttcacac cccggacgca gcgccttgtt
     2941 ggcataggtc acgtcgtgcc gcgcggtcca caagtggctg cgctcttcgg gtttggtcgc
     3001 ccacttgaac tcgccgccgc cgtgctccgc cgcgatcgac tgcaccagct ccgcctgctc
     3061 cttgacgcct gcttgggtgc cgtggaattc gatgaacagc gtgtccttga ccgggtagtt
     3121 cagtttcgag aacttgttca ccgagtccat ctgcacgtcg tccaacagct cgatgcgcgc
     3181 caccgggatg ccgagctgga tggtctggat cacggtgttg accgcgcctt cgatgctttc
     3241 gaaggagcat accgccgcag ccattgcctc ggggatgccg tacaaccgca gcgtcacttc
     3301 ggtgatgatg ccgagcgtgc cttcggagcc gacgaagagc cgcgtgagat cgtaaccggc
     3361 ggcggatttg cgcgcgcgtc gcgaggtgcg gatgatgcgg ccgtcggcga gcaccacggt
     3421 cagcgccagc acgttctcgc gcatggtgcc gtagcggacc gcgttggtgc cggacgcgcg
     3481 ggtcgccgcc atgccgccga gggaggcgtc cgcgccggga tcgatcggga agaacaggcc
     3541 ctggtcgcgg atgtgcgcgt tcaattcctt gcgggtgacg cgcgcctgca cggtgacgtc
     3601 gaggtcttcg ggattcacgc gcaggacctg gttcatgttc gccagatcca ccaccacgcc
     3661 gccctggtgg gggatgacgt tgccttcgag cgaggtgccg gtgccgtagg cgatgaccgg
     3721 ggttttgtgc cgggcgcaga tacggacgat ctcggcgact tcctcggtgg tgttcggaaa
     3781 caccaccgcg tccgggcggt gcgggtggtg ccaggattcg tccttgctgt ggtggtcgcg
     3841 caccccggtg ctggtggaaa gtcgttcacc gaaaagggcc ttcagctcgt cgatggcgga
     3901 ctggagcgct gttgcgggaa gactcattcg gatttcctcg ggaagatagg ctagatgtgg
     3961 cgggggaagc caaatcttac cgcaagggct cgttgtgtgc ctgtgtgtgc gctgcagatt
     4021 gcgagtaacc ctgtcatcag ctactttggg tccatgggaa tcgtgaaggc aacgggaatg
     4081 cgagttgtgt cagtcggcgc ggcggcaatc gcgctggtgt ccggcctggc ggtgccggcg
     4141 caactgggtg aaattccggt ggcggcgctt tcggccggca gttttaccgt cgcggttgcc
     4201 gacagtaccg cgttctccga accggcgccg gtgctggatt accgccagcg ggaaaaattt
     4261 ctccgtggcc gccatcactt caatcagaaa tgggtggttt tcccttctct cggtggcgac
     4321 tggggcctgg gtccgacgtt catcgctgac cgatgctcgg gttgccacgt cggtggcggg
     4381 cgcggcgacc ccccgaagtc ggcggaccag caactgcttt cggttctggt gcgcatttcg
     4441 atcccgggcg aggatgagca cgggggaccg aagccccacc cgaactatgg tgaccagatc
     4501 cagaaccagg gcctgatggg gcaggaccgc aacgcgacct atctgggcga acgcgtgccg
     4561 ccggaagcca gcctatacct cgactggcag gaagtgcccg gcaccttcgc ggacggcgaa
     4621 cgggtcatgt tgcgcaagcc gcggctgcgc gtggagaagc tgaatttcgg gccgctcggc
     4681 cccgaggtaa tgtactcact gaggatcgcg cagccgactt tcggactggg actgctcgaa
     4741 gcggtgagcg agaccgacat tctggcactg gcagagagac agaaatcgca aggcgtgaac
     4801 gggcgcccga attacgtctg ggacgcaatc ggcaaacgct tggcgctcgg acgattcggc
     4861 tggaaatcga accagccgtc gatcaggcag cagatcgccg cggcacttca cggcgacatc
     4921 ggtgtgacct cgtcgctgtt cgacaaggaa aactgcccgc cggtgcagga agattgcgcg
     4981 tatcagccgc cgggcaacca gccggaactc gttgatgtca actgggatga actggagttc
     5041 tggacccagg cgctcgctgt accggcgcgg cgcaacgtgg ttgaacccgt cttcgcccgt
     5101 ggagagcgtg ctttcaacga ggcgaagtgt tcggtctgcc atgtgccgga aatgaaagcc
     5161 ggggtcttgc cgggactgcc gcagatccag ggccaggtct tccatgccta caccgatctg
     5221 ctgctgcacg acatgggcga ggcgcttgcc gatggacgcc ccgacttcaa ggccggaccg
     5281 cgcgactggc gcaccccgcc gctttgggga ttggggttgt cgcagatagt gagcggcagc
     5341 accgcgatgc tgcacgacgg ccgcgcgcgc aacgccgctg aagctatcct ctggcacggc
     5401 ggcgaagcgg agggcgcacg cgaggcattt cgaaacatgc cgaaggcgga tcgggaggcg
     5461 ctgctcaagt ttctctcctc gatttgaacg gctattggag tgatatgcgc ccgatcatct
     5521 gttgctggcg cacgcagggc ggacgggcct gggtggcgat tcttgcgttc ggagcgcttg
     5581 cctgtctgcc gttcgaagcg gacgccggca caccggagga actgctcgcc cgctacgaag
     5641 gggaagcgcg caccgaagca ccggcatttc acggcttttc cgtcgaggaa ggaaggatgt
     5701 tctattttca gaaatacccg gtgccgattc tcggcgaagt cggctgcgta tcctgccatt
     5761 tgcaggaccc acgccggtcg gtcatgcgcc a
//
LOCUS       DASQOP010000062        10317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2642270, whole genome shotgun sequence.
ACCESSION   DASQOP010000062 DASQOP010000000
VERSION     DASQOP010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10317
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2642270"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VE030_02300"
     CDS             complement(<1..272)
                     /locus_tag="VE030_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonopyruvate decarboxylase"
                     /protein_id="HZF22278.1"
                     /translation="MKTHWSQRLHQLFRQQGIRQVSFVPDAGHATLIGLCEADRKLRA
                     VRLTTEEEGIALAVGAWLGGDKSVLLMQSSGVGNCINMASVLSACRV"
     gene            490..903
                     /locus_tag="VE030_02305"
     CDS             490..903
                     /locus_tag="VE030_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22279.1"
                     /translation="MTDSVRKVDYFYVEVANQAGAGATYLKALREAGVNLLAFTGFPS
                     GRKAQIDFIPEDTTAFRAAAKRNKWKLSARKTVFLLQGDDRLGAIHEVIGKLAAAKIS
                     ITALDAVTAGAGRYGAMFWVKPRDVAKAARILGAN"
     gene            complement(997..1983)
                     /locus_tag="VE030_02310"
     CDS             complement(997..1983)
                     /locus_tag="VE030_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZF22280.1"
                     /translation="MHAIRQWLFLLFLASVLLLAQEAVAADNFNRQDVTFTSVGKKLV
                     GWLYVPKGMKQGEKRATIVMAHGWSAVKEMYLDDFAARFSNGGFVVLVFDYRNFGDSE
                     GEPRGHIDPHMQHEDYRNAITWASLQPMVDASRIGIWGSSYSGAHVMHLAAFDRRVKC
                     AVAQVPLVNATDNFRRLVRADNWGGLQAWLAADRAEEYTSGKINYLPVVAEQGKPSAL
                     PTQDSYDWFIKVGQTRAPKWENRQTVRSLELALEYNPAANIHLISPTPFMMIVAENDV
                     LTPTDLAIEAFGRAREPKQLVILPGGHFDAYVSGFQGASTPALNWFKQHLMK"
     gene            complement(2186..2551)
                     /locus_tag="VE030_02315"
     CDS             complement(2186..2551)
                     /locus_tag="VE030_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22281.1"
                     /translation="MMIAEEPEFPQIMSDAEIDANVPVGEAVNQKIRRQPTDKELADD
                     AVALLKTLFPRIHQTLITLWGTGPGEDYLDGLIMDDRGNRQGFPPDVFRAVLILQRVH
                     FQTFGTFKKIDPWDVGRNP"
     gene            2866..3258
                     /locus_tag="VE030_02320"
     CDS             2866..3258
                     /locus_tag="VE030_02320"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZF22282.1"
                     /translation="MKIKSLLAAALLACIGLGVYYADLFEAAFLFNPIPRTAKSIARG
                     RQLFQQNCAVCHGAEGRGDGVAAASLRERPDDLSRIAPPPIFPDGIVAYRIKNGVKMM
                     PAFKTTLSENDTWDLINFIRSLSSPANR"
     gene            complement(3819..4637)
                     /locus_tag="VE030_02325"
     CDS             complement(3819..4637)
                     /locus_tag="VE030_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HZF22283.1"
                     /translation="MKTKASALRGFTRRDVLFGSGILLAGMLAGMARKTWPGPLTADS
                     ALIEDLVAANRILAALEIVDGYGHVSVRHDKDPNRYLIARSIAPEAVTANDVLELGLD
                     SKPVDGSDARMYLERFIHGEIYKARPEVKAVVHNHAAAVIPFGASNIPLRPIYHMAGF
                     VGRGVPIFDIRKVAGDTDMLVKTPALGAALARTLGDKPAALMRGHGAVVVGDSLPQAV
                     GRSYYLQMDAKLQAQAMALGGDITYLDAGEAARTEGFGGYERAWDLWKSKAMGK"
     gene            complement(4716..5132)
                     /locus_tag="VE030_02330"
     CDS             complement(4716..5132)
                     /locus_tag="VE030_02330"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HZF22284.1"
                     /translation="MFAFFLCFALTAACVLLHYETLRLLNDRLGRTNVISLRAKVLAA
                     LVGAMASHVLQIGAFALAYYLLRDKFGLGGFGGTFQDTLMSFLYFSSETYTTLGFGDI
                     YPTGALRMICGIESLIGLLMVSWTASFTYLEMRRYW"
     gene            complement(5272..6570)
                     /locus_tag="VE030_02335"
     CDS             complement(5272..6570)
                     /locus_tag="VE030_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZF22285.1"
                     /translation="MDPRFTPEFQRSTILKISWRLLPLIVVSYLVAYIDRTNVSFAAL
                     TMNKDLGISSFMYGLGAGIFFFGYALFEVPSNMVLERVGARRWIARIMITWGIISGLM
                     AAVNGPASFLALRFLLGVAEAGFFPGIIFYLSTWYPAAYRARVISTLFIAVPVANAVA
                     SVISGAILEMDGVWGLHGWQWVFILEAIPAVLLAFVVLRLLTDRPAIATWLGADQREW
                     LESEMQAERARVESAGRLTLLRALTDKRVLMLSLIYFTSVIASYGITFFLPQIVKGLG
                     HSNFITGLLTAIPYVIGVFGLVLWGHSSDRRNERRWHLIIASTVAAAGLAGVGWFTGS
                     YWALVAMSIATIGIYGSRPSFWPMPSMFLTGAAAAAGIALINSIGNLGGYVGPFIVGW
                     IKDSTKSFEMGLYFLAACAFASAVITYFATRATLGTTTKD"
     gene            7043..8176
                     /locus_tag="VE030_02340"
     CDS             7043..8176
                     /locus_tag="VE030_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZF22286.1"
                     /translation="MRRLFVLVLVVHLYMGWRLIPDTPFGVPGVVATALLLVLSLSVL
                     PFAEYARRNKQNPCADHLAGAGFLAMGFFSSLLILTLLREVVLLAGSAASGSLIEAAT
                     FRQLRIWSAAAVPLLAVLMTFVGFLNARRRAEVRTVEVPIAGLPEPLHGFSIVQITDI
                     HVGATIKNGYVSRIVDAVNELNADMIAVTGDMVDGSVGRLAPHTEPLGRLSARYGAFF
                     VTGNHEYYSGAHAWIAEMRRLGLLVLLNEHVVLEHFGESVVVAGVTDLSAHHFDPAHR
                     SDPMAAIAGAPEQAVKLLLAHQPRLAFAAAQAGFHLQLSGHTHGGQFFPWNFVVKLFE
                     PFSAGLHRLDQLWVYVSRGTGYWGPPKRFGAPSEITHLTLVPA"
     gene            complement(8210..8542)
                     /locus_tag="VE030_02345"
     CDS             complement(8210..8542)
                     /locus_tag="VE030_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HZF22287.1"
                     /translation="MPHSSGEQEMAKGYWIAHVDVNDPEGYKGYVAANGKAFAKYGAR
                     FLVRGGRFECKEGQSRSRNVVIEFKDYATAMACYNSPEYAEAMKQRINASVADMIIIE
                     GYDGPQPG"
     gene            complement(8554..9762)
                     /locus_tag="VE030_02350"
     CDS             complement(8554..9762)
                     /locus_tag="VE030_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014752177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HZF22288.1"
                     /translation="MKVKNIETLSCDAGWRNYHFVKLTTDTGITGWSEFDEGFGAPGV
                     GTAIERLSPRVTGQDVGAHERIYAELYSATRPASGGIIALAMGAIENALLDAKAKGLG
                     VPVYELLGGKIRDRIRVYWSHCATWRINHPAHYKPAITDLDGVKAIGREVREKRLSAL
                     KTNIFMYDQDGKNARGWRPGFGVPFAPELNVERDVLRNLVMHLEALRDGAGPDVDILL
                     DLNFNAKTEGYLKILRAIADLDIFWVEIDSFVPEALGYIRRHSPHPLSSCETLLGLRE
                     FMPYFRSQAMDVAIVDTPWNGVWQSMKIAAAAEAHEINVAPHNFYGHLCTMMNAHFAA
                     AVPNLRIMETDIDRIAWDHELFSQVPEFVDGHLVMPARPGWGIEPNEAAIRAHPPKGP
                     QGLLNYGRNK"
     gene            complement(10008..>10317)
                     /locus_tag="VE030_02355"
     CDS             complement(10008..>10317)
                     /locus_tag="VE030_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZF22289.1"
                     /translation="IETGSAYLRVVGPAYGFFGLGLALYFASQGAGRLFWPLLSGFVR
                     LIIATGGGWILQRLTGSLNWLFAALSLALVVYGVSLVAAIRSGAWFANDRVHIPRGHK
                     "
BASE COUNT         1965 a         3201 c         3170 g         1981 t
ORIGIN      
        1 acccggcagg cggacagcac cgaggccatg ttgatgcagt tgcccacgcc gctcgattgc
       61 atcagcagca cgctcttgtc gcccccgagc catgcgccga ccgcgagagc gatgccttct
      121 tcctcggtgg tgagccgcac cgcgcgcagt ttgcgatccg cttcacacag cccgatcagc
      181 gtggcatggc cggcgtccgg cacgaaactc acctggcgaa tgccctgttg cctgaacaac
      241 tgatgcagtc tctggctcca gtgggtcttc atggtgttct ggttattcgt ttgtatttgc
      301 atggttccgc gcgaccacgc aggataaacg cgatttggcc acagccggtg gaccggggca
      361 agatacggat accgaagcca ggtcgacggc gctttccggc gccggcagac ccggaaaact
      421 gaaagcccga cttgcgtgtg cgccagcaag tgctatagat aaatcaggcg tgcagaaaag
      481 gaggaggcca tgacagactc ggtgcgcaaa gtggactatt tctacgtgga agtggcaaac
      541 caggcagggg cgggcgctac gtatctgaag gcgctcaggg aagcaggcgt gaacctgctc
      601 gcattcaccg gattcccgtc ggggcgcaag gcacagatag acttcatccc cgaagacacc
      661 acggccttcc gcgccgcggc aaaaaggaac aagtggaagc tttcagcgcg caagacggtg
      721 ttcctgttac agggcgacga ccggctgggc gcgatacacg aggtgatcgg caagctcgcc
      781 gcggcaaaga tcagcataac cgcgctggac gcagtgacgg ccggggccgg acgctacggc
      841 gcgatgttct gggtcaagcc cagggacgtc gcgaaagcgg ccaggatcct cggggcaaac
      901 tgaagcggga tcgcgtcgcc ccgggctgca gtccgcgcgt agataaggag agcaccgacc
      961 ggtgcccgcc ctgtttgccg ttttgcccag ccgcgattat ttcatcaggt gctgcttgaa
     1021 ccagttcagc gccggtgtcg aggcgccctg gaagccgctc acataggcgt cgaagtggcc
     1081 gcccgggagt atcaccagtt gcttgggttc gcgggcgcgg ccaaacgctt cgatcgccag
     1141 gtcggtaggc gtgagcacgt cgttttcggc gacgatcatc atgaacggcg ttggagagat
     1201 aaggtgaata ttggccgccg ggttgtattc cagcgcaagt tcgagcgaac gcacggtctg
     1261 acggttctcc cattttggcg cgcgcgtctg gccaaccttg atgaaccagt cgtatgagtc
     1321 ctgcgtcggc agagccgagg gcttgccctg ttcggcgacc accggcaggt agttaatctt
     1381 gccgctggtg tattcctccg cccggtcggc agccagccat gcctgcagcc ctccccagtt
     1441 gtccgcgcgt accaggcggc ggaaattatc cgtcgcattg accagcggta cctgtgccac
     1501 cgcgcacttc acacggcggt cgaatgccgc aagatgcatc acgtgtgccc cgctgtaaga
     1561 cgagccccag atgccgatgc ggctcgcgtc caccatcggc tgcagcgaag cccaggtgat
     1621 cgcgttcctg tagtcctcgt gctgcatgtg cggatcgatg tgcccgcgcg gttcgccttc
     1681 gctgtcgccg aagttgcggt agtcgaagac cagcaccacg aagccgccgt tggaaaacct
     1741 ggcggcaaag tcgtcaaggt acatttcctt caccgcgctc cagccgtgcg ccatcacgat
     1801 agtcgcgcgc ttttctccct gtttcattcc cttgggtacg tacagccagc cgaccagctt
     1861 cttacccacg ctggtgaagg tcacgtcctg ccggttgaaa ttgtcggccg cgaccgcctc
     1921 ctgggcgagt agcaagaccg aagcaaggaa cagcagaaag agccattgcc tgatcgcgtg
     1981 catttttttc ctcctgtttg tttgagatat gccatgcggc tttaccggta tagacagcgg
     2041 tacggggcac gtcaatggca ggccgtactg cactgcaacg cgccggccgg ggagggcact
     2101 tcggatcggg ccggcaggcc ggaaagatag cggacacccg ctgatgcccg gctcgttttc
     2161 ggccttcacc aggccgcaat gccgctcagg gattccggcc gacgtcccag gggtcgatct
     2221 tcttgaatgt gccaaacgtc tggaagtgaa cacgctgcag gatcaacacc gcgcgaaaga
     2281 catctggagg gaatccctgg cgattgccgc ggtcgtccat gatcaagccg tccaggtagt
     2341 cctcacccgg tcccgttccc cataacgtga taagagtctg atggatgcgc ggaaacagtg
     2401 ttttcagcag tgcgaccgca tcgtcggcca gttccttgtc ggtcggttgc cgccgaatct
     2461 tctgattgac ggcctcgccg accggaacat ttgcgtcgat ttccgcatcg gacatgattt
     2521 gcgggaactc cggctcttca gcaatcatca cggcatgcct ccccagcttg caccgattcg
     2581 cagcagacga gttactggcc aaaagcggga atagcgttcg aggagagggg cgccgccgac
     2641 gagcgccggg gtgcctgacg gttccaccgt cgccctccct tcggaatgac ccgcgaaccg
     2701 cgccttgttg ttatttcagc aggcgattca gtgccagcgt attcctgcgc attcccagcg
     2761 tcaacgctgg accaagtact gccgttggcc ggctgattcc cggcccacgg cgcgacaacc
     2821 ggcagatgtt tggcctgtag ccatttgcgt ggcaaagtaa ccggcgtgaa gatcaaatcg
     2881 ttgttggcgg cggcactcct cgcctgcatc gggctgggtg tctactacgc cgacctgttc
     2941 gaagccgcct tccttttcaa tccgattccg cgaacggcga aatcgatcgc caggggaagg
     3001 cagctgttcc agcagaactg cgccgtatgc cacggcgccg aaggacgcgg cgacggcgta
     3061 gcggcggcct cgcttcggga acgtcccgat gacctgagtc ggattgcgcc gccgccgatt
     3121 tttcccgatg gcatcgtcgc ctaccggatc aagaacggtg taaagatgat gcctgccttc
     3181 aagacgacgc tgagcgagaa cgatacctgg gacctgatca atttcatccg atcgctatcc
     3241 tctcccgcca acaggtaagg gggcaatttg gcgcctacct gttgaccgcg gcgctcacgg
     3301 aatgcggacg ccgcgcactt gctttttcga ccgggacggg ctacaagaat atcagttgaa
     3361 caaatcttca cgccatcgaa agcgcctgat gacaaagcaa ggcgtaatgc acgggcacaa
     3421 agcgtcggac gcttgacgat gcctcattcc taatgaatac cgttccggaa aagatgaaaa
     3481 tgggaggtag ccactccacg caatgcacct gtcccgtctg tggacagagg agcggcaagc
     3541 actacccgca ctgccgttgt ccgcattgcg cgaaggtagg tggcagccat ctggcgagct
     3601 gtcgctgccc cgtctgcgga aaggttggtg gaaagcattt cgaccattgc tattgtccgg
     3661 agtgcggcga gggcggctga ctgactttga attcagaaaa acccgcacac aactagtgct
     3721 gcgaccgtct tcacccagga acagaaggca agcgcgacgc aaatcgattt tctcagtcac
     3781 ccgtcacccg tcaccggtct tcgctttgcg tcatcggcct atttgcccat cgccttgctc
     3841 ttccacaaat cccaggcgcg ttcgtagcct ccgaaacctt cggtcctggc ggcttcgccg
     3901 gcatcgagat aggtgatgtc gccgccgagc gccatggcct gtgcctgcag tttcgcatcc
     3961 atctgcaggt aataactgcg tcccaccgcc tgcggcagcg aatcgccgac caccactgct
     4021 ccgtggccgc gcatcaacgc ggcgggcttg tcgcccagcg tacgggcaag cgcggcgccg
     4081 agtgccgggg ttttcaccag catgtcggtg tcgcccgcga ccttgcggat gtcgaatatc
     4141 ggcacccccc gccctacgaa acctgccatg tggtagatcg gccggagcgg gatgttgctt
     4201 gcgccgaagg ggatgaccgc cgcagcgtgg ttgtgcacca cggccttgac ttcgggccgg
     4261 gccttgtaga tttctccatg aatgaagcgc tcgaggtaca ttctggcgtc gcttccatcc
     4321 accggcttgc tgtcgagacc gagctccagg acgtcgttcg cggtgaccgc ctccggggcg
     4381 attgagcgcg caatgagata acggttggga tccttgtcgt gccgcacgct cacgtggccg
     4441 taaccgtcca cgatctccag cgcggcaagg atgcggtttg ccgccaccag gtcctcgatc
     4501 aacgcggaat cggccgtaag cggccccggc cacgtcttgc gcgccatccc tgcgagcatg
     4561 ccggcgagaa gaatgccgct gccaaacaga acgtcgcgcc gcgtaaaacc gcgaagtgcc
     4621 gaagctttcg ttttcatgaa tcctccccgc aaacgagcat gattgaaatt cagcgagccg
     4681 caatttgcaa gcctgactcc gtccgtctct tgaccctacc agtaccgtcg catttccagg
     4741 taggtgaaag atgcggtcca actgaccatc aacagcccga tcagcgactc gatcccgcag
     4801 atcatccgca gcgccccggt cggataaatg tcgccgaatc ccagcgtcgt atacgtttcg
     4861 ctggagaagt aaagaaagct catgagcgtg tcctggaaag tcccgccgaa tccgcccaat
     4921 ccgaacttgt cgcgcagcaa gtagtaggcg agcgcgaacg cgccgatctg cagaacatgg
     4981 ctcgccattg ccccgaccag cgcggccagc accttcgcgc gcagcgagat gacgttggtg
     5041 cggcccagcc tgtcgttcag aagccgcagc gtttcatagt gcaacaggac gcaggctgcg
     5101 gtcagggcga agcacaggaa aaaggcgaac atggtgaagt cgttaccggt tgctctcgag
     5161 gatcgtcgga atctttgggg tcggctcaaa aatacccgat caataacgcc atacagccca
     5221 gtcggtgcac agcacgacgt gagtaccgtg gacggccatc ggctgacgga cttagtcctt
     5281 cgtcgtagtc cccagtgttg ccctggtcgc gaaatacgtg atcactgccg aagcgaacgc
     5341 acacgccgcc aggaaataca gtcccatctc gaaactcttg gtgctgtcct tgatccagcc
     5401 gacgatgaag ggtccgacat aaccgccgag gttgccgatg gaattgatca gcgcaatgcc
     5461 ggcggcggcc gccgctccgg tgaggaacat cgagggcata ggccagaagg acggccgcga
     5521 cccgtagatg ccgatggtgg cgatcgacat ggcgaccaga gcccagtagg aaccggtaaa
     5581 ccagcccacg cctgcgagtc ctgccgccgc gaccgtagat gcgatgatga ggtgccagcg
     5641 cctctcgttg cggcgatccg aagagtggcc ccaaagcaca aggccgaaca ccccgatcac
     5701 atacgggatc gcagtcaaca ggcccgttat gaagttcgaa tgcccgagcc ccttgacgat
     5761 ctgcggcagg aaaaaggtaa tgccgtagct ggcgatgacg ctcgtaaagt agatcaacga
     5821 cagcatcagt acgcgcttgt cggtgagcgc ccgcagcagc gtcaggcggc cggcgctttc
     5881 gacccgggct cgctcggcct gcatttcgga ttcgagccac tcgcgctggt ccgcgccgag
     5941 ccaggtggct atggccggcc ggtccgtgag cagcctcagt acgacgaaag cgagcagcac
     6001 tgcgggaatg gcttcgagga tgaaaaccca ctgccaaccg tgcaggcccc agacgccatc
     6061 catctccaga atcgcgcccg aaatgactga cgctaccgca ttggcgacgg gcactgcaat
     6121 gaacaacgtc gagatcaccc gggcacgata cgcagccgga taccaggtac tgagatagaa
     6181 gatgatgccg gggaagaatc cggcctcggc cacgccgaga agaaagcgca gcgcgaggaa
     6241 actggccggc ccgttgacgg ctgccatcag cccggaaatg atgccccagg tgatcatgat
     6301 gcgggcgatc cagcgccgcg cgccgacccg ctccagaacc atgttgctcg gcacctcgaa
     6361 cagggcatag ccgaagaaga aaatgcccgc gcccaggccg tacatgaaag agctgattcc
     6421 caggtccttg ttcatggtca gcgcggcaaa ggagacattg gtgcgatcga tgtacgcgac
     6481 cagatagctc accacgatca gggggagcag ccgccacgag atcttaagga tcgtgctgcg
     6541 ctggaattcg ggcgtgaatc gcggatccat tgcgttggcc tccctgctcg agatgctttt
     6601 accggcacct ggcgccggtc ccgatcgttt gtgtgccgcg cgaccgctgc ccgcgcggag
     6661 atttgacctg tggcgcgtgc cgggacccgg cgcgcaacag atgtggcgga tattaccgga
     6721 tcatcgaaag gtcacgggct ccaactgcgg ccacccgtgc aggcgtgcgg ccgatggctg
     6781 agtccgtcgc gggttcgcca ggaaagcgca ggatttcgac agggtgccgg attcagggtt
     6841 tgatatcccg gatttctgca ctatgcatgc agccatgctc cgccgcacga gccgggcgtc
     6901 ccgtgcccgc cagctccagg cccaaggatt gtaacgaccg gaccggcccg gtacttgtaa
     6961 tacgccccgg actcccccat ttcggaactg ttgtctctgc gccttgcagt gacgtccgac
     7021 cgccccagaa aagcgggcca ccatgcgccg actgtttgtt ctggtcctcg tggtccatct
     7081 ctacatggga tggcgcctga ttccggacac gcctttcggt gtgccgggcg ttgttgcgac
     7141 ggctttgttg ctggtcctct cgctttccgt cctgccattt gccgagtacg cgcgccggaa
     7201 caagcagaat ccctgtgccg atcatctggc tggtgcaggc ttcctggcga tgggattttt
     7261 ttcgtcgctg ctgatcctga cgttgctgcg cgaagtggtc ctgcttgcgg gttctgcggc
     7321 aagcgggtcg ctcattgagg cggccacctt ccggcagttg cgcatctgga gtgcggctgc
     7381 ggttccgcta ctcgcggtgc tgatgacgtt cgtaggattc ctcaacgcgc gccgtcgggc
     7441 ggaggttcgg actgtagagg ttccgatcgc aggcttgcct gaaccgctgc atggtttcag
     7501 tatcgtgcag atcaccgata ttcatgtcgg cgccaccatc aagaacggct acgtcagcag
     7561 gatcgtggat gccgtcaacg aactgaatgc ggacatgatt gcggttacgg gcgacatggt
     7621 cgacggaagc gtcgggcggc tggccccgca caccgaaccg ctcggacggc tgtccgcgcg
     7681 ctacggcgct ttcttcgtca ccgggaatca tgagtactat tccggtgcac atgcctggat
     7741 tgctgaaatg cgccgcctcg gcctgttggt gctcctgaac gagcatgtcg tgctcgaaca
     7801 cttcggggag tcagtcgtgg tcgccggggt caccgacttg agcgcgcatc atttcgatcc
     7861 agcgcatcgc agcgatccga tggcggccat cgccggcgca cccgagcagg ccgtgaaact
     7921 tcttctcgcg caccagccga ggttggcttt cgctgcggcg caggccggct tccacctgca
     7981 gttgtcaggt catacgcacg gtggccagtt ctttccctgg aattttgtcg tgaagctgtt
     8041 cgagccgttt tctgcgggat tgcatcgtct ggaccagctc tgggtctacg taagccgcgg
     8101 cacaggctat tggggaccgc cgaaacgctt cggcgcacct tccgaaatca cccacctgac
     8161 gctggtaccc gcttgaagtt gagatggggc gagttatcgt taccagcccc tagccgggct
     8221 gagggccgtc gtaaccttcg atgatgatca tgtcggcgac cgaggcattg atccgttgtt
     8281 tcattgcctc ggcatattcc ggcgagttgt aacaggccat tgccgtagcg tagtccttga
     8341 actcgatgac cacgttgcgc gagcgcgact gcccttcctt gcactcgaac cggccgccgc
     8401 gcaccaggaa gcgcgcgccg tacttggcga aagccttccc gttcgcagcc acatagcctt
     8461 tgtagccctc gggatcgttc acgtcgacat gcgctatcca gtaccccttc gccatctcct
     8521 gctctcccga tgaatgcggc atgcgaattg gtgctatttg ttgcggccgt agtttagcaa
     8581 gccctgcggg cccttcggcg gatgggcgcg gatcgccgct tcgttcggtt cgattcccca
     8641 gccgggccgc gcgggcatga ccagatgacc gtccacgaac tccggcactt ggctgaacaa
     8701 ctcgtggtcc caggcaatgc ggtcgatgtc ggtctccatg atgcgcaggt tgggcaccgc
     8761 cgccgcaaag tgcgcgttca tcatggtgca gagatggccg tagaagttgt gcggggcaac
     8821 gttgatctcg tgcgcttcgg cggccgccgc gattttcatc gactgccaca ccccgttcca
     8881 gggcgtgtcc acgatggcga cgtccatcgc ctggctgcgg aagtagggca tgaactcgcg
     8941 cagtcccagc agcgtctcgc acgaactgag gggatgaggg ctgtgccggc ggatgtaacc
     9001 cagagcttcc ggcacgaagc tgtcgatctc cacccagaag atgtcgagat cggcgatggc
     9061 gcgcaggatc tttagatagc cttcggtctt ggcgttgaag ttgaggtcga gcaggatgtc
     9121 cacgtcgggg ccggcgccgt cgcgcagtgc ttcgaggtgc atgaccagat tgcgcagcac
     9181 gtcgcgttcg acgttgagct cgggtgcgaa cggcacgccg aaacccggcc gccatccgcg
     9241 cgcgttcttc ccgtcctggt cgtacatgaa gatgttggtt ttcagcgcgc taagccgctt
     9301 ctcgcgtact tcccggccga tcgccttgac cccgtcgagg tcggtgatcg ccggcttgta
     9361 gtgggccggg tgattgatcc gccaggtcgc gcaatgcgac cagtacacgc ggatgcggtc
     9421 gcggatcttg ccgccgagga gttcatagac cggcacgccg agccccttcg ccttcgcatc
     9481 gagcagcgcg ttctctatcg cacccatggc gagcgcaatg atgccgccgg aagcagggcg
     9541 ggtggcgcta tagagttcgg cgtagattcg ctcatgcgcg ccaacgtcct ggccggtgac
     9601 gcggggggac agccgctcga ttgcggtacc cacgcccggc gcgccgaagc cttcgtcgaa
     9661 ctcgctccag ccggtgatgc cggtatccgt ggtcagtttg acgaaatggt aattgcgcca
     9721 gcctgcatcg caggacagtg tttcgatgtt ctttaccttc atattctcct ccccctctcc
     9781 ccagccctct ccccagccct ctcccgccag gggagaggga gttcttgttg ctctgccgga
     9841 gtcgaaggtt atctcaaaaa gttctcctga cttctatcct caagttccag tgctgcacgc
     9901 agacgaggtt caatttcccc tctcccctgg cgggagaggg ttggggagag ggggggcggc
     9961 gatcaccagc ggccaggtgg aaacgcggac ggtcttggcg ggttcggtca tttgtgccct
    10021 cgaggaatgt gcacccggtc gttggcgaac cacgcgccgg aacggatcgc cgcgacaagc
    10081 gagaccccgt agacgacgag tgccagggaa agcgccgcaa acagccagtt caacgacccg
    10141 gtgaggcgct gcaggatcca gccgccgccg gtcgcaatga tgaggcggac gaagccgctg
    10201 agcaaaggcc agaacaggcg gcccgcgccc tgcgaggcaa aataaagcgc caggcccagg
    10261 ccgaagaatc cgtacgcggg gccgacgacg cgcagatagg cgctgccggt ttcgatc
//
LOCUS       DASQOP010000063         2080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2241143, whole genome shotgun sequence.
ACCESSION   DASQOP010000063 DASQOP010000000
VERSION     DASQOP010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2241143"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VE030_02360"
     CDS             complement(<1..377)
                     /locus_tag="VE030_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HZF22290.1"
                     /translation="MIKLFYASDTCSLASHIALEDAGADYSTARISFASDEQRKPEYL
                     AINPKGRAPALVTDKGVLTETPAILAFIAQSFPQARLAPLDDPFRFAEVQAFNSYLCS
                     TLHVAHAHRMRGNRWADDPAAIAA"
     gene            complement(484..822)
                     /locus_tag="VE030_02365"
     CDS             complement(484..822)
                     /locus_tag="VE030_02365"
                     /inference="COORDINATES: protein motif:HMM:NF018717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HZF22291.1"
                     /translation="MRLTFPCLETLRRTTLSKAYWIVRVSVRNEQRYPEYLAAAGPAF
                     QKFGANFIVRGGKFETMEGDARERNVVVEFKDRATALACYQSPEYQGARAIRQKYADA
                     DFIIIDGVAD"
     gene            902..1783
                     /locus_tag="VE030_02370"
     CDS             902..1783
                     /locus_tag="VE030_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007744038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZF22292.1"
                     /translation="MDLSDLRIFSAVVREGGVTRAAERLHRVQSNVTTRIRQLEEDLS
                     VALFIREGKRLHLAPAGQVLLDYADRLLALADEARGAVQDTRPRGTFRLGAMESTAAV
                     RLPGLLSEYHRRHPGVVLELRTGNPQVLGGAILAGDLDAALVAEPIADAPFEKTFAFD
                     EELVIVAAANHPPIGKSGGLPRTIIVFEHGCPHRKRLEEWYASRREMPERTIELASYH
                     AMLGCVVAGMGIALIPKSVLTTFPERKRLSVHLLARAENCAQTVLIWRKGAGSANVRA
                     LEEILRGRSPHLQQKSK"
     gene            1877..>2080
                     /locus_tag="VE030_02375"
     CDS             1877..>2080
                     /locus_tag="VE030_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22293.1"
                     /translation="MPKKFTDPEAIAEDIIRDVGTNLVVGLPLGLGKANHIVNALYAR
                     AAADRAISLTLFSALTLEKPKPSN"
BASE COUNT          413 a          608 c          644 g          415 t
ORIGIN      
        1 gccgcgatcg cggccggatc atcggcccag cgatttccac gcatgcggtg agcatgggcc
       61 acgtgcaacg tcgagcacag atagctgttg aaggcctgca cctccgcgaa gcgaaaggga
      121 tcgtccagcg gcgccagccg cgcctgcgga aagctctggg cgatgaaggc gaggatcgcg
      181 ggcgtctcgg tcagaacgcc cttgtcggtt accaatgccg gagcgcgtcc cttgggattg
      241 atggcgagat attccggctt gcgctgctca tcgctggcga agctgatccg cgccgttgag
      301 taatctgcac cggcgtcctc gagcgcaata tgcgatgcca gcgagcaggt atcggaagcg
      361 taaaacagtt tgatcatggc gttacttttc cctcgacggg tgtctgccga attcatagct
      421 cgtacggcgc tgatgtcaca gtggtgcgga tacagcttcc ggataggtcg cgacgccgcc
      481 gtctcaatcg gcgacgccgt caatgatgat gaaatcagcg tccgcgtact tctggcgaat
      541 agccctcgcg ccctgatatt ccgggctctg atagcaggca agggcggtcg cgcggtcctt
      601 gaattcaacc acgacatttc gttcgcgggc gtctccttcc attgtttcga actttccgcc
      661 gcgcacgatg aagttcgctc caaatttctg gaacgcaggg ccggccgcgg caaggtattc
      721 gggatatctc tgctcgttcc ggaccgagac gcgtacaatc caataggcct ttgacaaggt
      781 cgttctcctc agtgtttcca gacaggggaa ggttaacctc acccgtgaca cagcctttac
      841 gcgaggcgga tcatctaata aaatgaataa ttatgatgag actgatcttg tggagagatg
      901 aatggatttg tcggatctga ggatcttcag cgcggttgtg cgcgaaggcg gcgtcacgcg
      961 cgcggcagaa cggctgcatc gcgttcagtc caatgtcacg acgcgtatcc ggcagcttga
     1021 ggaggatcta agtgtcgcgc tcttcattcg tgagggcaaa cggcttcacc tcgcgccggc
     1081 tggtcaggtg ttgctcgact acgcggaccg ccttcttgcc cttgccgatg aagcgcgcgg
     1141 agccgtgcag gacaccaggc cgcgcgggac gtttcggctc ggcgcgatgg agagcaccgc
     1201 ggccgtcagg ctgccggggc tgctcagcga ataccaccgg cggcatcccg gggtggttct
     1261 ggagctgcgc accggcaacc cacaggtttt gggcggcgcc atcctcgcgg gcgatctcga
     1321 cgcggcactc gttgccgagc caatcgccga tgcgccgttt gagaagacgt tcgcctttga
     1381 cgaagaactg gtgatcgttg cggccgccaa tcatccgccg atcggcaaga gcggcggtct
     1441 gcctcgaacc atcatcgttt tcgagcacgg ctgcccgcat cgaaagcggc ttgaagaatg
     1501 gtatgcaagc cgtcgcgaga tgccggagcg caccatcgag ttggcgtcct atcacgccat
     1561 gctgggatgc gtcgttgcgg gcatggggat agcccttatc cccaaaagcg tgctgacgac
     1621 atttccggag cgaaagcgtc taagcgttca ccttctggct cgagcagaaa actgtgcgca
     1681 gaccgtcctg atttggcgca agggagcggg ctcggcgaac gtcagggcat tggaggagat
     1741 attgcgaggc cgcagcccac atctgcagca aaaatccaaa taacgagtag ggaagtataa
     1801 tactttcaac ccctaacgtt cgattcctca gggactgccg gatgttccgg ttcgcgtcgc
     1861 gccagacggg atattcatgc cgaaaaaatt cactgacccc gaggcgattg cggaggatat
     1921 catccgcgat gtcgggacca acctggtggt gggactgccg ctcgggctcg gcaaggccaa
     1981 tcacatcgtc aacgcgctct acgcgcgcgc cgccgccgac cgcgcgatca gcctgacctt
     2041 gttttccgcg ctgacgctgg aaaaaccgaa gccgtcgaat
//
LOCUS       DASQOP010000064        13377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1233870, whole genome shotgun sequence.
ACCESSION   DASQOP010000064 DASQOP010000000
VERSION     DASQOP010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13377
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233870"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..159)
                     /locus_tag="VE030_02380"
     CDS             complement(<1..159)
                     /locus_tag="VE030_02380"
                     /inference="COORDINATES: protein motif:HMM:NF020471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HZF22294.1"
                     /translation="MFNPSREDVRRFFFETWRKYRTQAPLEGLEKTALEVALLHPEYH
                     ALLDSEDRN"
     gene            complement(358..999)
                     /gene="nth"
                     /locus_tag="VE030_02385"
     CDS             complement(358..999)
                     /gene="nth"
                     /locus_tag="VE030_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HZF22295.1"
                     /translation="MNPAKRAEIFRRLREANPAPTTELAYQTPFELLVSVVLSAQATD
                     KSVNLATARLFPVANTPRAILALGESGLTPFIRSIGLYRTKAKNILAACRILLEKFGG
                     EVPRRREDLESLPGVGRKTANVILNTAFGEPTIAVDTHIFRVANRTGIAPGKDVLEVE
                     NKLMKFVPEEFRKDAHHWLILHGRYVCIARKPACPACLIRDLCEFRGKSKDEG"
     gene            complement(1183..1881)
                     /gene="rsxB"
                     /locus_tag="VE030_02390"
     CDS             complement(1183..1881)
                     /gene="rsxB"
                     /locus_tag="VE030_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HZF22296.1"
                     /translation="MITLVDAIDALLPQTQCTKCGYQGCRPYAEAIARGDADINQCPP
                     GGAAAVLKLAQLLGREQKALNPANGVEKPRAAALIDESRCIGCMLCIHACPVDAIVGS
                     PKQMHTVLTEFCTGCDLCVPPCPVDCIEMVELEALAERGNRHATALAGQSVEAIAAIA
                     LNRYQFHQLRLEHEKVEREQRLAKKTGAMLARLESLTIGHDLDRKKAAVRAAIERARA
                     RLAGQNDTPFKTKS"
     gene            complement(2063..5596)
                     /locus_tag="VE030_02395"
     CDS             complement(2063..5596)
                     /locus_tag="VE030_02395"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZF22297.1"
                     /translation="MNISVDAILTQPELRLGFGSRFEDLYQRAGLQQLDTAFLDFLGS
                     AEAPLRARLESGRIEPEALARKDESELLIAVAEHLDDFIARLFGIEAEVQALSARHHE
                     LAPLFSCKRLFVQRKAMNSYKPEVAAGLNGEELGQKLEQWFGEKFSELAFARHVAEWQ
                     RDEAANAEELDTAKRYAAWAVHALAGQRKHQTGVLFKAPKKLDFQKLVPLDTREENGV
                     TVHTLSHLRRRHGFALTDSGTDLVGALDQTHYCIWCHEQGRDSCSHGLKEKAPADGSA
                     AQFKTTVFGVPLAGCPLEERISEFQKLKTEGIAIGALAMIVVDNPMVAATGHRICNDC
                     MKSCIYQKQEPVNIPQAETRSLKDVLELPWGFEIYSLLTRWNPLDLRRPFPRPTSGRR
                     VLVAGMGPAGFTVAHHLMNDGHSVVGIDGLKIEPLSAHQSGVDRTGARVAFEPIFDSE
                     SLFESLDDRVMAGFGGVAEYGITVRWDKNFLKLIRLLLQRRQEFTLFGGVRFGGTLSV
                     DDAFDLGFDHVVLAAGAGRPTVLDIPNGLAPGVRTASDFLMALQLTGAAKQNSIANMQ
                     LRLPVAVIGGGLTAIDTATESAAYYPLQVEKFLHRFETLCAERGEEKVRASWSAAEKS
                     IAEEFLTHARAIRAERNKAAAEGREPHILELLQSWGGVTIAYRKRLIDSPSYTLNHEE
                     VEKALEEGIRFAENLTPVAVEVDELGYAKGLTVTSKRTLADGSTETATVTLPARTVLV
                     AAGTQPNTVLAREDAHNFHLDGRYFQACDENGLPVKPEKSAKPASAHVLLSKRPDGRF
                     VSFFGDLHPSFFGNVVKAMGGAKQGYPVVSRVLEKVLPAFSGTDGDFVAALNSELRAT
                     VHEVIRLTPTIVEVVVRAPLAAKKFRPGQFYRLQNFETLAHHVDGTRLAMEGLALTGA
                     WVDRDKGLVSTIVLEMGGSSDLCALLKPGEPVILMGPTGSPTEIHSGEIVVLVGGGLG
                     NAVLFSIGHALRAAGSKVLYFAGYKKMIDRYKIMDIEAASDVVVWCCDEAPGFEPNRP
                     DDRAFVGNIVQAMAAYAQGNLGPQAIPFKAADRIIAIGSDRMMGAVAAARHGVLAAHL
                     DPHHFAIGSINSPMQCMMKEICAQCLQPHVDASSGERTYVFSCFNQDQPLDRVDFPGL
                     AARLVQNSVQEKLTAHWIDRCLTHLSRRPIV"
     gene            complement(5687..6727)
                     /locus_tag="VE030_02400"
     CDS             complement(5687..6727)
                     /locus_tag="VE030_02400"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF003645.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HZF22298.1"
                     /translation="MLYRLARPLLFQLDPERSHHGALRFLDVACRFGLSGAIAGAKVH
                     SPRRVMGLEFPNPVGLAAGLDKNGEHIDSLAALGFGFIEVGTVTPRPQPGNPAPRLFR
                     IPGARAIVNRMGFNNDGVERLLDNVARARYRGILGINIGKNFDTPIERAAEDYLYCLR
                     KVYAAASYIAVNISSPNTAKLRQLQQSDQLDRLLGTLTEESRKLAVRHGRTVPLAIKI
                     APDLNAEEIGQMAASFVRHGVNAVIATNTTLSRDGVEGLENSGQAGGMSGAPLKIRST
                     AVLCELARSLAGRIPIIGVGGIFSAADAREKLNAGASLVQLYSGLIYEGPGLARRIAA
                     ELTVSLKSLNPL"
     gene            complement(6782..7531)
                     /locus_tag="VE030_02405"
     CDS             complement(6782..7531)
                     /locus_tag="VE030_02405"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HZF22299.1"
                     /translation="MTQLNDLPFSVLQFYATAPYPCSYLPDKLARSQVATPSHAIDSQ
                     VYSELVKVGFRRSGAFTYRPYCDHCRACVPVRIVTAGFEPSRSQRRAYARHDALEARM
                     LELKYHPEHYALYLRYQSHRHSGGGMDHDSREQYRHFLLQSNVRSSLVEFREEGRLRM
                     VSIVDRLDDGLSSVYTFFEPEVAGASLGTYNVVWQVQLCRRLGLPYLYLGYWIAQSRK
                     MAYKINFQALEGLIDGQWRRLPRDASSPEAA"
     gene            complement(7528..8226)
                     /gene="aat"
                     /locus_tag="VE030_02410"
     CDS             complement(7528..8226)
                     /gene="aat"
                     /locus_tag="VE030_02410"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HZF22300.1"
                     /translation="MIPWLGSGDPFPEVERALAEPNGLLAAGADLSLPRLLEAYRSGI
                     FPWFANDQPILWWSPDPRTVLFPAEMKVSRSLARTMRSTRFEVRADTAFDEVIEACRQ
                     PRRGESGTWITEAMAAAYGALHRAGFAHSVETWLDGNLVGGLYGVALGKAFFGESMFT
                     RVSEASKVALVALARQLEHWEFGVIDCQMNTAHLASFGAREIPRAEFTRRLRELVHYA
                     PVPLPWRLEPTIAR"
     gene            complement(8245..8790)
                     /locus_tag="VE030_02415"
     CDS             complement(8245..8790)
                     /locus_tag="VE030_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HZF22301.1"
                     /translation="MKTTKWALVGVCVLLTSCSTVSNLFTRNPSATAVLAPTKGNSVS
                     GTVNFTQKGAVVLVEAKITGLPANGTHGFHIHEKGNCSAADASSAGAHFNPSGDKHGG
                     PVGAVRHGGDLGNLRADANGYAHASIQVMGITLGTDANSIIGRAVIVHGGPDDLKSQP
                     AGNSGPRVACGLISKNPDKFF"
     gene            9034..9531
                     /locus_tag="VE030_02420"
     CDS             9034..9531
                     /locus_tag="VE030_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011657354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZF22302.1"
                     /translation="MTRWAGLIAAGGEALDLACGSGRHSRYLAAAGYRVCAIDRDAQA
                     LAALTGVAGVSVQVADLERAPWPLKGRRFAGIVVANYLYRPLFPRLLEALAEDGALIY
                     ETFALGNERFGKPSNPDFLLRPGELLELARGKLRVLAYEDLQLSLPKPAMVQRICAVG
                     PRWSP"
     gene            9590..10498
                     /gene="dapA"
                     /locus_tag="VE030_02425"
     CDS             9590..10498
                     /gene="dapA"
                     /locus_tag="VE030_02425"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HZF22303.1"
                     /translation="MLKGSLVAIVTPMLEDGSLDLERLRRLIDFHIREGSNGIVVVGT
                     TGESPTVDFEEHCLLIRTAIEHAAARTPVVAGTGANSTREAIELAAYAKAAGADMSLS
                     VVPYYNKPTQEGLYRHFRAIAEAVDLPQILYNVPGRTVADMQNDTVLRLAQVPNIVGL
                     KDATANMERGAEMLRRAPRSFAIYSGDDGTALPLMMLGAHGVISVTANAAPRLMQEMC
                     AAVFAGDLPKARAHNNKLLGLHRHLFVEANPIPVKWVLQQMGLIDGGIRLPLTPLSSS
                     FHDLLRGAMEQAEIAGAAVAQASMAK"
     gene            10550..11680
                     /gene="bamC"
                     /locus_tag="VE030_02430"
     CDS             10550..11680
                     /gene="bamC"
                     /locus_tag="VE030_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HZF22304.1"
                     /translation="MNSTQFFVAAVTAVVALSGCSGKLIEGKKIDYKSAGKVNPLQVP
                     PDLSAPPTSDRYTIPETAAGSATYSEYSEGQGARHTDQAATAAAVVLPASEKVRIERG
                     GSQRWLVVEADSVTLWPAIREFWQDTGFLIDKELPDIGIMETDWAENRAKIPESTLRK
                     LIGKVIDQAYSYPERDKFRTRLERGEEPGTTEIYISHRGAYQVVVGEHGSEQVKWQMR
                     PTDPELEAEMLSRLMRRLGAKEEQVQAKAPVAAPQAQLAKNGDNPEALVLADSFDRAW
                     RRVGLALDRVGFTVEDRDRSKGLYYVRYLDPDIDGKGKSGSRWSRLAFWRSDTGKKDE
                     QFRISVADADKGSEVRVQNADGASAKSTTAGRILALLQEELK"
     gene            11723..12490
                     /locus_tag="VE030_02435"
     CDS             11723..12490
                     /locus_tag="VE030_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZF22305.1"
                     /translation="MRFASLGSGSRGNALVVEAGSTRILLDCGFGLGETTARLGRLGL
                     TPEDLSAVVVTHEHEDHIGGVARLARRHSIPVWLTHGTLTGLEALFSGIGNLHLIDNY
                     GGFSIGDLRVEPFPVPHDAREPAQYVFGDGSRRLGVLTDTGCSTPHIEAMLSGCDALV
                     LECNHDPEMLRVSSYPQKLRERIAGRYGHLDNGAASELLARLDSSRLKHLVAAHLSQE
                     NNRPELARAALAGAIACDPGWIEVADQDNGLGWRAIG"
     gene            complement(12629..13120)
                     /locus_tag="VE030_02440"
     CDS             complement(12629..13120)
                     /locus_tag="VE030_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22306.1"
                     /translation="MAENATPQHTPLLGNAEYEAALDTALAKAQRSIRIFEHTLGRGY
                     NSPQRFELLHRFLLASRRNRLQIVAHEPQTIDRNCPRILNLLRLHSHSISIHETHSNA
                     KSVYDPFTVVDDHSFVHRFHFDEMRGLLALDDPIGTHVLAERFDEIWEASSPTVSATT
                     IGL"
     gene            complement(13129..>13377)
                     /locus_tag="VE030_02445"
     CDS             complement(13129..>13377)
                     /locus_tag="VE030_02445"
                     /inference="COORDINATES: protein motif:HMM:NF042820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix domain-containing protein"
                     /protein_id="HZF22307.1"
                     /translation="RSAHNRGLELALPAQMLYSGSRLFINGESLVINAGEAQPLRALA
                     DARRLAGHEVPRTGTAAEALYRWYRSGYILPATNAIAT"
BASE COUNT         2413 a         4086 c         4376 g         2502 t
ORIGIN      
        1 attgcggtct tcgctatcca gcagcgcgtg atactcgggg tgcagcagcg ccacttcgag
       61 cgcggttttc tccaatcctt ccaaaggggc ctgcgtgcgg tatttgcgcc aggtctcgaa
      121 aaagaaacgg cgcacgtctt cgcgcgacgg attaaacatg agagcaagta tgaaggaaca
      181 aggatgaagg cggaaggatg aaggatgaag gcggaagtat gattgcacgc aagctcatgc
      241 agagcgttcg ggtaccggac acaattcaag gcgcacggtt ttgtgagcgt ttctccaggc
      301 gagaggtcca atccttcatc cttcatcctt catccttcat ccttcatcct tcatccttca
      361 tccctcatcc ttgctcttcc ccctgaattc gcaaagatcg cggatcaggc aggccggaca
      421 tgccggcttg cgcgcgatac atacgtaacg cccgtgcagg atcagccaat ggtgcgcatc
      481 cttgcggaat tcctccggga cgaatttcat caacttgttc tcgacttcga ggacgtcttt
      541 gccgggggcg atgccggtcc ggttggcgac gcggaagata tgggtgtcca ccgcgatggt
      601 cggttcgccg aatgcggtat tgaggatcac attcgcggtt ttacggccca ctcccggcag
      661 gctctccagg tcttcgcgcc ggcgcggcac ctcgccgccg aacttttcca gaagaattct
      721 gcaggcggcg aggatgttct tcgccttggt ccggtacaag ccgatgctgc gaatgaaggg
      781 cgtgagtccc gattcgccga gcgcgaggat cgcccgcggc gtattggcga ccgggaaaag
      841 tctggcggtg gcgaggttca cgcttttgtc ggtggcctgg gcggacagta cgaccgaaac
      901 cagcaactcg aacggcgttt gatacgcgag ttcggtggtc ggcgccggat tggcctctcg
      961 cagcctgcga aagatttccg ctcgtttagc gggattcatc gtcgagactc cgggaggaaa
     1021 cgtcttacca cgaaggcacg aagacacgaa gaaagtcaaa tgaacgcgaa acggccgggt
     1081 tggtttccct gccgggtttc tctgtgatac tgcgcactcc gaggcgcacc gcgacgattt
     1141 ggcccggttt ccttcgtgtc ttcgtgcctt cgtggtgaga tttcaagatt ttgtcttgaa
     1201 gggagtatcg ttctgtccgg cgaggcgggc gcgggcgcgt tcgatggcgg cgcgcactgc
     1261 agccttcttg cggtcgagat cgtggccgat cgtcagcgat tccaggcgcg caagcatggc
     1321 cccggttttc tttgccagcc gttgctcgcg ctccaccttt tcgtgttcga gtcgaagctg
     1381 gtggaattgg tagcggttca gggcgatggc cgcgatggcc tcgacgcttt gtccggccag
     1441 cgccgtcgca tgccggttgc cgcgttcggc cagcgcctcc agctcgacca tttcgatgca
     1501 gtcgaccggg cagggcggaa cgcacagatc gcagccggtg caaaactcgg tcagcacggt
     1561 gtgcatctgc ttggggctgc cgacgatcgc gtccaccgga caggcatgga tgcacagcat
     1621 gcaaccgatg cagcgcgatt cgtcgatcag cgccgcggcg cgcggttttt cgactccgtt
     1681 ggcgggattg agagcctttt gctctcgtcc gagcaactgc gccagcttga ggacggcggc
     1741 cgccccgccc ggggggcact gattgatgtc ggcgtcgcct cgcgcgattg cctcggcgta
     1801 aggccggcaa ccctgatagc cgcacttggt gcactgggtc tgcggcagca gcgcgtcgat
     1861 cgcgtcaacc agagtaatca tcggcagaac tcaccacaga gacacagagg cacagagaaa
     1921 gtccaaggca aaaaaattga aataaatgca agactcgata ggtaaacacc gccatccacc
     1981 gactgctaca tacctccgtt tctccaggat aaaatccaag tgccttctcc gtgcctctgt
     2041 gtctctgtgg ttggactgag gtctaaacta tgggccgacg gctgaggtga gtcaggcagc
     2101 ggtcgatcca gtgggcggtg agtttttcct gcaccgagtt ctggacgagc cgcgccgcca
     2161 gtccggggaa atccacccgg tcgagcggtt gatcctggtt gaagcaggag aacacgtagg
     2221 tgcgctcgcc gctggacgca tccacgtgcg gctgcaggca ctgggcgcag atctccttca
     2281 tcatgcactg catcggtgag ttgatcgagc cgatcgcgaa gtgatgcgga tcgaggtgcg
     2341 ccgccagcac gccgtggcgc gccgcagcca ctgcgcccat catccggtcg gagccgatgg
     2401 cgataatgcg atctgccgct ttgaagggga ttgcctgagg gccgagattg ccttgtgcgt
     2461 acgcggccat cgcctgcacg atgttgccca cgaaggcgcg atcgtccggg cggttcggct
     2521 cgaatcccgg cgcctcgtcg cagcaccaca ccacgacgtc ggaggccgct tcgatgtcca
     2581 taatcttgta gcggtcgatc attttcttgt agccggcaaa gtacaggacc ttcgacccgg
     2641 ctgcgcgcaa cgcgtggccg atcgagaata gcacggcatt gccgaggccg ccgccgacca
     2701 gcaccacgat ttcgccggaa tgaatttcgg tgggcgagcc ggtcgggccc atcaggatca
     2761 ccggttcgcc gggcttgagc agcgcgcaca gatcggacga cccgcccatc tccagcacga
     2821 tggtggacac cagccccttg tcgcgatcca cccaggcgcc ggtgagcgcc agaccttcca
     2881 tcgccaggcg ggtgccatcg acatgatgcg ccagggtctc gaagttctgc agccggtaga
     2941 actgaccggg ccggaatttc ttcgcagcca gcggcgcgcg caccaccact tcgacgatgg
     3001 tgggcgtgag ccggatcacc tcgtgtaccg tcgcgcgcaa ttcgctgttg agcgccgcga
     3061 cgaaatcgcc atcggttccg gaaaaagccg gaagcacctt ctccagaacg cggctgacca
     3121 cggggtagcc ctgcttagcg ccgcccatgg ccttgaccac attgccgaag aaggaggggt
     3181 gcaggtcgcc gaagaagctg acgaatcgtc cgtcggggcg cttcgacagc agcacgtgcg
     3241 cggaggccgg cttggcgctt ttttcgggtt tcaccggcag gccgttctcg tcgcaggcct
     3301 ggaagtaacg gccgtcgaga tggaagttgt gcgcgtcctc gcgcgccagc acggtgttcg
     3361 gctgggtgcc tgcagcgacc agtactgtgc gcgccggaag cgttaccgtc gctgtctccg
     3421 tgctgccgtc tgcgagtgtg cgcttgctgg tgacggtgag ccccttggcg tagccgagtt
     3481 cgtccacttc gacggcaacg ggcgtgaggt tttcggcgaa acggatacct tcctcgagcg
     3541 ctttttcgac ttcctcgtga ttgagcgtgt acgaagggct gtcgatcagg cgtttgcgat
     3601 aggcgatggt caccccgccc caggattgca gcagctcgag gatatgcggc tcgcggcctt
     3661 ctgccgcggc cttgttgcgc tccgcgcgaa tggcgcgtgc gtgggtcaga aactcttcgg
     3721 caatactctt ttccgcggca ctccatgaag cgcgaacttt ttcctcgccg cgttcagcgc
     3781 atagcgtctc gaagcgatgc aggaatttct ccacctgcag cggatagtaa gcagccgatt
     3841 cggttgccgt gtcgatcgcg gtgaggccgc cgccgatgac cgccaccggc agccgcagtt
     3901 gcatgttggc gatcgaattc tgcttggcgg cgccggtgag ttgcagcgcc atgaggaagt
     3961 cggaagcggt gcgcacgccc ggcgccagac cgttgggaat gtcgagcacc gtggggcgcc
     4021 ccgcacccgc agccagcacc acgtgatcga acccgagatc gaaggcgtcg tcgaccgaaa
     4081 gcgtgccgcc gaagcgcacc cctccgaaca acgtgaattc ctgacggcgt tgcagcagca
     4141 gccgtatgag tttcaggaag ttcttgtccc agcgtaccgt gatgccgtat tcggccactc
     4201 cgccgaagcc ggccatgacc cgatcgtcca gcgattcgaa cagcgattcg gagtcgaaga
     4261 tcggctcgaa agcgacccgc gcgccggtac gatccactcc ggactggtgt gcggacagcg
     4321 gctcgatctt caggccgtct atgccgacca cgctatggcc gtcgttcatc agatgatgcg
     4381 ccacggtgaa tccggccggc cccataccgg cgaccagcac gcgccggccg ctggtggggc
     4441 gggggaaagg ccggcgcaag tcgagcgggt tccagcgcgt gagcaggctg tagatctcga
     4501 atccccaggg cagttccaat acgtccttca ggctgcgggt ttccgcctgc gggatgttga
     4561 ccggctcctg cttctggtag atgcaggatt tcatgcagtc gttgcagata cggtgcccgg
     4621 tggcggcaac catggggttg tccaccacga tcatcgccag cgcgccgatc gcgatgcctt
     4681 cggtcttgag cttctggaat tcggaaatgc gctcttccag cggacagccc gccagaggca
     4741 cgccgaaaac cgttgtcttg aactgcgctg cgctaccgtc cgcgggggct ttctccttca
     4801 gtccgtgcga acaggaatcg cggccttgct cgtggcacca gatgcaatag tgggtctgat
     4861 cgagcgcgcc gaccaggtcg gtgccggaat cggtgagcgc gaagccgtgg cggcggcgca
     4921 ggtgcgaaag cgtgtgcacc gtgacgccgt tttcttcacg tgtgtcgagc ggcaccagtt
     4981 tctggaagtc gagttttttc ggcgccttga acagcacgcc ggtctggtgc ttcctttgcc
     5041 ccgccagggc gtgcaccgcc cacgctgcgt agcgctttgc ggtgtcgagt tcttcggcat
     5101 tggcggcttc gtccctctgc cattcggcta cgtgccgggc gaacgccagt tcgctgaatt
     5161 tttcaccaaa ccactgctca agcttctgcc caagttcctc gccgttgaga ccggcagcga
     5221 cctcgggctt gtaggagttc atcgccttgc gctggacgaa caagcgtttg caggagaaca
     5281 gtggtgcaag ctcgtggtgg cgcgccgaca gcgcctgcac ctcggcctcg atgccgaaca
     5341 ggcgcgcgat gaaatcgtcc agatgttcgg ccacggcgat aagcaattct gattcgtcct
     5401 tgcgcgccag tgcctccggt tcgatgcggc cggattccag acgcgcgcgc agcggtgcct
     5461 cggcagagcc gagaaaatcg aggaaagcgg tatcgagctg ctgcagtccg gcgcgctggt
     5521 agagatcttc gaagcggctg ccgaagccga gcctcagttc tggctgggta aggattgcgt
     5581 ctacgctgat gttcattcag gggcttctgt gacaggggcg gggcgccgat aaaagcgcgc
     5641 attataacgc tggcaggtcg aaatcttatt cgagctgacg ctaaagttat aacggattca
     5701 ggctcttaag gctcaccgtg agttctgcgg cgatccggcg cgccagtccc gggccttcgt
     5761 aaatcagccc gctgtagagt tggaccaggc tggcgccggc gttgagtttt tcccgcgcgt
     5821 cggccgcgga gaagataccg cccacaccga tgatcgggat gcgcccggcc aatgatcgtg
     5881 ccagttcgca caagactgcg gtcgagcgaa tcttcagcgg cgcgccgctc atcccgccag
     5941 cctgcccgct gttctccagc ccttcgacgc catcgcggga aagggtggtg ttggtggcga
     6001 tcaccgcgtt cacaccgtgc ctgacgaacg aggccgccat ctggccgatt tcctcggcat
     6061 tcaggtcggg tgcgatcttg attgccagcg gcaccgtcct gccgtgccgc accgcaagtt
     6121 tgcggctctc ttcggtgagc gtgcccagca gccggtcgag ctggtcggac tgctgtagct
     6181 ggcgcagctt ggcggtgttg ggcgaggaga tgttgaccgc gatgtagctc gcagccgcat
     6241 acactttgcg caggcagtac aggtaatcct cggcggcgcg ctcgatcggg gtgtcgaagt
     6301 tcttgccgat gttgatgccg aggatgccgc gatagcgcgc gcgcgcaaca ttgtcgagca
     6361 gtcgctccac gccatcgttg ttgaaaccca tgcggttgac gatggcgcga gccccgggga
     6421 tgcggaacag gcgcggagcc gggttgccgg gttgcgggcg cggggtcacc gttccgacct
     6481 cgatgaagcc gaaacccagc gccgccagcg agtcgatatg ttcgccgttc ttgtccagtc
     6541 ccgccgccag tcccaccgga ttgggaaact ccagtcccat cacgcggcgc ggcgaatgaa
     6601 ctttcgcgcc ggcgattgcc cctgagagcc cgaaccggca ggcaacatcc aggaaacgca
     6661 gcgcgccatg gtgcgaacgc tcagggtcga gctggaatag gagcgggcgg gccaggcgat
     6721 aaagcatttc agaaggcggc ggcaactgcg ccgccgtccg gtggtacccg gtgtgcagag
     6781 atcacgcggc ctcgggggag gacgcgtcgc gcggcagtcg tcgccattgg ccgtcgatca
     6841 atccctccag cgcctggaag ttgatcttgt aggccatctt gcggctttgc gcgatccagt
     6901 agcccagata gagataagga agccccaggc gccggcacag ctgcacctgc cacaccacgt
     6961 tgtaagtacc gaggctggcg ccggcgactt cgggttcgaa gaacgtgtat accgacgaca
     7021 ggccgtcgtc gagcctatcg acgatgctga ccatgcgcag gcgcccttcc tcgcggaact
     7081 cgaccaggct ggagcggacg ttgctctgca ggaggaagtg gcggtactgc tcgcggctgt
     7141 cgtgatccat gccgccgccg ctatggcggt gcgactgata gcgcaggtac agcgcgtagt
     7201 gctcagggtg atacttgagt tccagcatgc gcgcttccag cgcatcgtga cgggcgtagg
     7261 cgcgacgctg cgagcggctg ggctcgaagc cggcggtgac gatgcgcacc ggcacgcagg
     7321 cgcggcagtg atcgcagtag ggacgatagg taaacgcgcc gctgcggcgg aagccgacct
     7381 tcaccagctc gctgtaaacc tggctgtcga tcgcatggct tggcgtggcc acctgagagc
     7441 gggccagctt gtcgggcagg tagctgcacg ggtacggggc agttgcgtag aactgcagta
     7501 ccgaaaaggg caggtcgttg agctgtgtca tcgcgcgatc gtaggctcca gacgccacgg
     7561 cagcggaacc ggggcatagt gtaccaattc ccgaagcctg cgcgtgaact cggcgcgcgg
     7621 gatctcgcgg gcgccgaagg aggcgaggtg ggcggtgttc atctggcagt cgatcacgcc
     7681 gaattcccaa tgctccagtt ggcgcgcgag cgccacgagc gcgaccttgg aggcttcgct
     7741 gacgcgggtg aacatggact cgccgaagaa cgctttgccc agcgccacgc cgtacaggcc
     7801 gcccaccaga ttgccgtcga gccaggtttc caccgaatgc gcgaacccgg cgcgatgcag
     7861 cgcaccgtag gctgcggcca tcgcttcggt aatccaggtg ccggactccc cgcgccgcgg
     7921 ttgccggcaa gcttcaatca cctcgtcaaa agccgtatcg gcgcgcactt cgaagcgcgt
     7981 gctgcgcatc gtgcgcgcca gcgagcgcga gaccttcatc tcggcgggga acaggaccgt
     8041 gcgcggatcg gggctccacc acagtatcgg ctggtcgttg gcgaaccagg ggaagatgcc
     8101 gcttcggtag gcttccagca gcctaggcaa cgacagatca gcgccggcgg caagcaggcc
     8161 gttcggctcc gcgagtgcgc gctcaacctc gggaaaagga tcgccggagc ccagccaggg
     8221 aatcaaggtg cgataagcgc acgctcagaa aaatttgtcg gggttcttgc tgatgagtcc
     8281 gcaggccacg cgcggacccg agttgccggc cggctgcgac ttcagatcat caggaccgcc
     8341 atgcactatc acggcgcggc cgataatgct attggcgtcg gtacccagcg tgatccccat
     8401 gacctggatc gacgcgtgtg cgtagccatt ggcgtcggcg cgcaggttgc cgagatcgcc
     8461 gccgtgacga accgcgccga ccggaccgcc gtgcttgtcg ccggacggat tgaaatgcgc
     8521 tccggcgctg gaagcgtcgg cggcgctgca gttgcctttc tcgtggatat ggaagccgtg
     8581 cgtgccgttg gccgggagcc cggtgatctt agcttcgacc agcacgaccg cgcctttctg
     8641 ggtgaagttg acggtgccgc tgaccgaatt gcccttggtg ggcgccagta ccgcggtggc
     8701 ggagggattc ctggtgaaca ggttgctcac ggtgctgcag gacgtcagca gcacgcatac
     8761 gccgaccaat gcccacttgg tagttttcat tgtgttctcc aagaacgtcg tctttccggt
     8821 taaagggcaa ctccaaccga ggtttttagc accggccccg ggaggtgtca aatcagcttg
     8881 tgccagcttg tgttcctgca tatgcaaaca taatttgcgc gagtccaagg cggccggcac
     8941 gcgggaaagt tgtagcattg agaatgtccc cgcgccccga ttgcatttga cgtcttggat
     9001 aacgcgcacg cgccgcaatc cccctctgaa tgggtgacgc gctgggccgg tctcattgcc
     9061 gccggcggcg aagcgctcga ccttgcctgt ggcagcggcc ggcacagccg ctacctcgct
     9121 gccgccggct atcgcgtctg cgcgatcgat cgcgatgccc aggcgcttgc agcgttgacc
     9181 ggtgtggccg gcgtcagcgt gcaggtcgcg gacctggaac gcgcgccgtg gccgctcaag
     9241 ggccgtcgct ttgccggcat tgtcgttgcc aactacctgt atcggccgct gtttccgcgt
     9301 ctccttgagg ccttggcgga ggacggcgct ctcatctatg aaacgttcgc gctcggcaac
     9361 gaacgcttcg gcaagccgtc caatccggat ttcttgctcc gacccggcga gctgctcgaa
     9421 ctggcgcgcg gaaaattgcg cgtactcgcc tatgaagacc tgcagttgag cctgcccaaa
     9481 ccggcgatgg tgcagcggat ctgtgccgta gggccacgct ggagtccgta gcgctccggc
     9541 tgcggaaaga atcggttaaa atcgcacttt caattgtatg ggggccgcca tgttgaaggg
     9601 aagcctggtc gccatcgtca cgccgatgct cgaggacggc agcctcgacc tcgaacgcct
     9661 gcgccgtctg attgatttcc acattcgcga aggcagcaac ggtatcgtcg tcgtcgggac
     9721 caccggcgag tctccgacag tggatttcga agagcattgc ctcttgatcc ggaccgccat
     9781 cgagcacgcg gccgcccgta ccccggtcgt cgccggtacc ggtgccaatt cgacgcgcga
     9841 ggccatcgag cttgcggcgt acgccaaggc cgccggcgca gacatgagcc tgtcggtggt
     9901 gccctattac aacaagccta cccaggaagg gctgtaccgc cacttccgcg ccattgccga
     9961 ggcggtggac ctgccccaga ttctctacaa cgtgccgggt cgcacggtag cagacatgca
    10021 gaacgacacg gtgctgcgcc tggcgcaggt tccgaatatt gtcggcctga aggatgcgac
    10081 cgcaaacatg gagcgcggtg cggagatgtt gcggcgcgcg ccgagaagtt ttgcgatcta
    10141 cagcggggat gacggcaccg cattgccgct gatgatgttg ggcgcgcacg gcgtgatctc
    10201 ggtgaccgcg aatgcggcgc cgcggctgat gcaggagatg tgcgcggccg ttttcgccgg
    10261 cgatcttccc aaggcccgcg cccacaacaa caagctgctc ggcctgcacc ggcatctgtt
    10321 cgtcgaggcc aacccgatcc cggtgaagtg ggtgctacag cagatggggc tgatcgacgg
    10381 cggcatccga ctgccgctga cgccgctgtc ttcgtccttt cacgacctgt tgcgcggcgc
    10441 aatggagcag gccgagatcg ccggcgccgc ggttgcgcag gcttccatgg ccaagtgaac
    10501 caatcgagcc ggcaatggtc gttgatgcaa acctggtgaa tcctcacgca tgaattcaac
    10561 gcagtttttt gttgcagccg tcacggcggt cgtcgcactc tccggttgtt ccggcaagct
    10621 catcgaaggc aagaagatcg actacaagtc ggcgggtaag gtgaacccgc tccaggtgcc
    10681 gcctgatctg agcgcaccgc ccaccagcga tcgttacacc atccccgaga ctgcggccgg
    10741 ttcggcgacc tactcggagt attccgaagg acagggcgcc cgacataccg atcaggccgc
    10801 gaccgcggcc gcggtcgtcc tgcccgcatc ggaaaaagtg cgtatcgaac gcggcggcag
    10861 ccagcgctgg ctggtggtgg aagcggattc ggtgacattg tggccggcga tcagggagtt
    10921 ctggcaggac accggattcc tgatcgacaa agaactaccc gacatcggga tcatggagac
    10981 ggactgggcg gaaaaccgtg ccaaaattcc cgaaagcacg ctgcgcaaac tgatcggcaa
    11041 ggtgatcgac caggcctatt cctaccccga gcgcgacaaa ttccgcaccc gcctggaacg
    11101 cggcgaagaa cccggcacga ccgaaattta tatcagccat cgcggcgctt accaggtcgt
    11161 ggtcggggag cacgggagcg aacaggtcaa atggcaaatg cggccaaccg acccggaact
    11221 cgaggcggag atgttgtcgc ggttgatgcg caggctggga gcgaaggaag agcaggtcca
    11281 ggcgaaagcg ccggtggccg ctccgcaggc gcaactggcg aagaatggtg acaatccgga
    11341 agcactggtg ctggccgaca gtttcgatcg cgcgtggcgg cgggtaggcc tggcgcttga
    11401 ccgcgttggc ttcacggtcg aggatcgcga ccggtcgaaa ggcctctact acgtgcgcta
    11461 cctggatccg gacatcgacg gcaagggcaa gtccggaagc cgctggtccc ggctggcgtt
    11521 ctggcgcagc gacaccggca agaaggatga gcaattccgg atcagcgtgg ccgatgcgga
    11581 caagggcagc gaggtcaggg tgcagaacgc cgatggcgcg agtgcgaaat ccacaaccgc
    11641 agggcgcatc ctggcgctgc tgcaggaaga gttgaagtaa attcgacgtg atgacaccgg
    11701 cgacgccgaa gtgagcggca agatgcgctt cgcatcgctg ggcagcggca gtcggggcaa
    11761 tgccctggtc gtcgaagccg gttccacccg gattctgctc gactgtggat tcgggctcgg
    11821 cgagacgaca gcccgtctcg gccgccttgg cctcaccccc gaagatctgt cggccgtcgt
    11881 cgtcacccac gagcacgaag accatatcgg cggtgtcgcg cggctggcgc gccgccattc
    11941 cattcctgtt tggctcaccc atgggaccct gaccggcttg gaagcgctgt tctcaggcat
    12001 cggcaacctt catctaatcg acaattacgg cggcttttcg atcggcgacc ttcgggtcga
    12061 accgtttccg gtgccgcacg atgcgcgcga gccggcgcag tacgtattcg gcgatggctc
    12121 gcgccggctc ggggtgctca cggacaccgg ttgttccacg ccgcacatcg aggccatgct
    12181 gtcgggctgc gacgccctgg tgctggaatg caatcacgat cccgaaatgc tgcgagtgag
    12241 cagctatccg cagaaactgc gcgaacgcat cgccgggcgt tacggccatc tcgacaacgg
    12301 ggcggcctcg gagttgctgg cgcgcctcga ttcgagccgc ctgaagcatc tggtggccgc
    12361 gcacctgagc caggaaaaca accggcccga gctggcgcgc gccgcgcttg ccggcgcgat
    12421 cgcttgcgat cccgggtgga tcgaagttgc cgaccaggac aacggcctgg ggtggcgcgc
    12481 gatcggttga cgccgcttcg caaatctgcc gctgaaatga aaaaagccgg tcggaaaccg
    12541 gctttttttc gccgtagcga cgggattact tcttaatata aaggcgcgcc cgattgccac
    12601 gaaatgtaac caggacacaa gcgggggctt acaagccgat cgtggtggcg gaaacggtgg
    12661 gggaggaggc ttcccatatt tcgtcgaagc gctcggcaag cacgtgggtg ccgatcggat
    12721 catccagcgc gagcaggccg cgcatctcgt cgaagtggaa gcggtgcacg aagctgtgat
    12781 cgtccaccac ggtaaagggg tcgtagaccg actttgcgtt cgagtgggtt tcgtggatgc
    12841 tgatcgagtg gctgtgcagg cgcaacagat tgagaatgcg cgggcaattg cggtcgatcg
    12901 tctgcggctc gtgcgccacg atctgcagtc ggttgcgccg gctggccagc agaaaccggt
    12961 gcagcagttc gaagcgctgc ggcgaattgt atccgcgtcc cagcgtgtgc tcgaagatgc
    13021 ggatggagcg ctgcgccttc gccagcgccg tatccagggc ggcttcgtat tcggcgttgc
    13081 cgagcagtgg ggtgtgttgc ggggtcgcgt tttccgccat ttgcacgctc aggttgcgat
    13141 cgcgttggtc gccggcaaaa tataaccgct gcgataccac cggtaaagtg cttccgcggc
    13201 cgtgcccgtc cgcggaacct cgtggcccgc caggcgccgc gcgtcggcga gagctcgcaa
    13261 cggctgcgcc tcgccggcgt tgatcaccag cgactctccg ttgatgaata gcctggagcc
    13321 gctgtagagc atctgcgcag gtaatgccaa ttccagccca cggttgtgcg cactgcg
//
LOCUS       DASQOP010000065         3073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_225898, whole genome shotgun sequence.
ACCESSION   DASQOP010000065 DASQOP010000000
VERSION     DASQOP010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225898"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /locus_tag="VE030_02450"
     CDS             complement(<1..1032)
                     /locus_tag="VE030_02450"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HZF22308.1"
                     /translation="MASNVVVIGTQWGDEGKGKVVDWLTDHAQGVVRFQGGHNAGHTL
                     VIGGKKTVLHLIPSGILRPGVRCYIGNGVVVSPQALLDELAMLESAGVEVQGRLSISD
                     ACPLILPYHAALDQARESAKGEGKIGTTGRGIGPAYEDKVARRAIRLQDLFHRERFAA
                     KLGEVLDYHNFVLKQYFNARPVDFQKTLDQALAVAERFRPMVGDVPRLLFEAGKRGEN
                     LLFEGAQGTLLDVDHGTYPYVTSSNCVAGAAAAGSGVGPQALHYVLGITKAYTTRVGG
                     GPFPTELYDDVGRHLASRGNEFGATTGRARRCGWFDAAALKRSIQINGVSGLCVTKLD
                     VLDGVETLQV"
     gene            complement(1066..2226)
                     /locus_tag="VE030_02455"
     CDS             complement(1066..2226)
                     /locus_tag="VE030_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HZF22309.1"
                     /translation="MQKWLLPEYIEDILPAEAARVERLRRRILDLFFSHGYELVIPPM
                     LEYLDSLLTGTGRDLDLRTFKLVDQLSGRMLGLRADITPQVTRIDAHLLNRQGVTRLC
                     YSGTVLHTQPSGLLRTREPLQIGAELYGHAGLESDIEIQTLMLRALQAAGLQGIHLDL
                     GHVSVFRALVTRASIAPEQEAELFRVLQAKDVPALRELSAGLEAKTREALLLLPQLYG
                     GAEVLATARQRLPAYPELAACLNLLEAIGRQLSGEVKELCFDLAELRGYHYHSGVVFA
                     AYAGQRPEAIARGGRYDEVGRAFGRARPATGFTIDLRDLADMGNGDLRPMRVLAPYRP
                     GDAALQTEVARLRAGGNIVVVELPGHEANRAELGCERQLVARAGVWTLEPLN"
     gene            complement(2300..2494)
                     /locus_tag="VE030_02460"
     CDS             complement(2300..2494)
                     /locus_tag="VE030_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HZF22310.1"
                     /translation="MEGHTSILLTAVALMLIIEGVLPFLLPAIWRDAFRRLTEMSDGQ
                     IRFVGLSSMLAGLLLLFLTK"
     gene            complement(2581..>3073)
                     /gene="hflC"
                     /locus_tag="VE030_02465"
     CDS             complement(2581..>3073)
                     /gene="hflC"
                     /locus_tag="VE030_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HZF22311.1"
                     /translation="RTVHDVVSGERDKIMEVMREKVDDDASKIGVKVLDVRLKRVDLT
                     QEVSEAVYRRMEAERKRVANELRSTGFAEAEKIRADADREREVIIAQAYRDAQRIKGD
                     GDAKASAIYARAFQQNPEFYAFYRSLEAYRQSFRNKGDLLILEPSSEFFKYLKNSSGR
                     SGK"
BASE COUNT          535 a         1057 c          940 g          541 t
ORIGIN      
        1 gacctgcagc gtctccacgc catcgagtac gtcgagcttg gtgacgcaca atcccgacac
       61 gccgttgatc tggattgaac gcttcagcgc cgccgcgtcg aaccagccgc aacgccgcgc
      121 ccgcccggtc gtcgcaccga actcgttgcc gcggctggcg aggtgcctgc cgacgtcgtc
      181 gtagagttcg gtcgggaacg gcccgccacc cacccgggtc gtataggcct tggtgatacc
      241 gagcacgtag tgcagcgcct gcgggccgac accgcttccc gccgcggcgg cgccggcgac
      301 acagttgctc gaggttacgt agggataagt gccatgatcg acatccagaa gcgtgccctg
      361 cgcgccttcg aacagcaggt tctcgccgcg cttgcccgcc tcgaacagca ggcgcggcac
      421 gtctcccacc atggggcgaa agcgttccgc cacggccagc gcctggtcca gcgtcttctg
      481 aaaatccacc gggcgtgcgt tgaagtattg cttgagcacg aagttgtgat agtcgagcac
      541 ttcacccagt ttcgcggcga aacgctcccg atggaacaag tcctggagac ggatcgcgcg
      601 ccgcgccacc ttgtcctcgt aggcagggcc gatgccgcgc ccggtggtgc cgatcttgcc
      661 ctcgcccttg gcgctctcgc gcgcctggtc gagcgccgcg tgatagggca ggatcagcgg
      721 gcaggcatcg ctgatggaga gccgcccctg tacttcgaca ccggcgctct ccagcattgc
      781 cagttcgtcc agcagcgcct gcggcgacac caccacgccg ttgccgatgt agcagcgcac
      841 tccgggccgc agaatcccgg acggaatcag gtgcagcacg gttttcttgc cgccgatcac
      901 cagcgtatgc ccggcgttat ggccgccctg gaatcgcacc acgccctgag cgtgatcggt
      961 cagccagtcg acgactttcc ccttgccctc gtccccccac tgcgtgccga tgaccacaac
     1021 attgctcgcc attaccacct ctggttcagg aatccgttgc gtcgatcaat tcaggggctc
     1081 gagcgtccat actccggcgc gcgcgaccaa ttgccgttcg caacccagtt cggcgcgatt
     1141 ggcttcatgt cccggcagtt ccaccaccac gatgttcccg ccggcacgca gccgcgccac
     1201 ttcggtctgc agtgccgcgt cgccgggccg gtaaggcgcg agaacgcgca tcggcctgag
     1261 atcgccgttg cccatgtcgg cgaggtcgcg caagtcaatg gtaaatccgg tcgccggcct
     1321 ggcgcggccg aaggcacgcc ccacttcgtc gtagcggccg ccgcgcgcaa tcgcttccgg
     1381 gcgttggccg gcataagcgg cgaacaccac cccgctgtga tagtggtagc cgcgcagctc
     1441 ggccagatcg aagcacaact ccttgacctc gccgctgagc tgcctgccga tcgcttccag
     1501 cagattgagg caggcggcca gttccggata cgccggcagg cgttggcgcg cagtcgccag
     1561 cacttctgcc ccgccgtaaa gctggggcag aagcaacaaa gcctcgcgcg tcttggcctc
     1621 gaggcctgct gaaagctcgc gcagcgccgg cacgtccttc gcctgcagca cccggaacag
     1681 ctccgcctcc tgctccggcg cgatagaggc gcgggtcacc agcgcgcgaa acaccgagac
     1741 gtggccgagg tcgagatgga tgccctgcag ccccgcggcc tgcagtgcac gcagcatcag
     1801 cgtctggatt tcgatgtcgc tttccagacc ggcatggcca tagagctctg cgccaatctg
     1861 cagcggctcc cgggtgcgca acaacccgga aggttgcgtg tgcagcaccg taccgctata
     1921 acacaggcgg gttacgccct ggcggttgag caggtgcgcg tcgatacgcg tcacctgcgg
     1981 cgtgatgtcg gcgcgcaaac ccagcatccg tcccgacaac tggtccacca gcttgaaggt
     2041 cctgagatcg agatcgcggc cggtgccggt gagcagcgaa tcgaggtact cgagcatggg
     2101 cggaatcacc agctcgtagc cgtgtgagaa gaacaggtcg aggatgcggc ggcgcagccg
     2161 ttccacccgc gccgcttctg cgggaaggat gtcctcgatg tattcaggaa gcagccactt
     2221 ctgcatggtc atttgcaagc gaacgatcgc ggtcctggcc cgctggcggc gcggccactg
     2281 acaaagcggc tatgttgggc tatttcgtca ggaaaagcag cagcaggccg gcaagcatcg
     2341 aggacagccc gacaaagcgg atctggccgt cggacatttc ggtgagccgc cgaaacgcgt
     2401 ccctccatat ggccggaagc agaaacggca acacgccttc tatgatcagc atcaacgcca
     2461 ctgccgtcag caggatgctc gtatggcctt ccatcctccc ctttccggcc gcgaaaccac
     2521 ccccgcgcag cgcagcgcgg acgagaccag ccctctcctg gtctcacggc ctgcggcttg
     2581 ttatttgccg ctccggcctg aggaattctt caggtacttg aagaattccg aactcggctc
     2641 gagtatcagc aggtcgccct tgttgcggaa actctggcga tacgcctcga ggctgcggta
     2701 aaaggcgtaa aactcgggat tctgctgaaa cgcgcgggcg tagatcgcgg aggctttagc
     2761 atcgccgtca cccttgatac gctgcgcgtc gcggtaggcc tgcgcaatga tcacctcgcg
     2821 ttcgcgatcg gcatcggcgc ggatcttctc ggcctcggcg aaaccggtgg agcgcaactc
     2881 gttggcgacc cgcttgcgct ccgcctccat gcgccggtac accgcctcgc taacttcctg
     2941 cgtgagatcg acccgcttga ggcgcacgtc cagcactttg acgccgatct tgctggcatc
     3001 gtcgtcaact ttctcgcgca tgacttccat gatcttgtcg cgctcgcccg acaccacgtc
     3061 gtgcacggtg cgc
//
LOCUS       DASQOP010000066         7068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_428653, whole genome shotgun sequence.
ACCESSION   DASQOP010000066 DASQOP010000000
VERSION     DASQOP010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7068
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428653"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VE030_02470"
     CDS             complement(<1..675)
                     /locus_tag="VE030_02470"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HZF22312.1"
                     /translation="MVSMHGLLIAAMAAAIAAALPRTARPAELPLWEAGAGVAVISFP
                     EYRGSDQRRTFVLPVPYLVYRGEYLKADRDSVRGQFLKNDRVELSLSVNGSIPVDSTD
                     NAARRGMPDLDPTLELGPNLLLTILRKPDRSMEFGLRLPARTVIATDFSHTKNVGWVF
                     QPQINIDFRDTWLGKGWNVGFAAGPLYGDRRYHNYFYGVAPAFATPQRPAFNAAGGYA
                     GSQILGA"
     gene            complement(675..2114)
                     /locus_tag="VE030_02475"
     CDS             complement(675..2114)
                     /locus_tag="VE030_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HZF22313.1"
                     /translation="MGLPFAAGNIRHTTRIRFLTGNVDDSIAILRCGDAGVSAPAAAQ
                     SAASHCDVFVLGGGPAGSTIAALLAERSWNVVIAEKERHPRFHIGESLLPLNMPLLRQ
                     LGVYEQVKGIGMPKYGAEFNSPEHGPPVTFDFSQAWDKTYPNALEVRRSEFDEILFRN
                     CGAKGARTFEECLITNVEFRARQDVRIDARMSNGGERTWRARFFVDASGRDTFLANRF
                     GIKRRNRSHNSAAIYGHFTGAQRLPGKAAGNISMFWFDHGWFWFIPLLDGTTSIGAVC
                     WPYYMKSRQTDPTTFFLDTIAMAPGLAERLRDAKLTHGVTATGNFSYQADRMRGERYI
                     MIGDAFAFIDPVFSSGVMLAMNSAFLGADAVDASLRDAPQAPALLRKFDRSVRSGVGG
                     FSWFIYRMTLPAMRDMFMHPSNRLRMQEAVLSLLAGDLFRGTPIHLSLRAFKSLYYLL
                     NLVYPRRSFAAWRHRRRTLRERMAQTPGS"
     gene            2153..2878
                     /gene="fabG"
                     /locus_tag="VE030_02480"
     CDS             2153..2878
                     /gene="fabG"
                     /locus_tag="VE030_02480"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HZF22314.1"
                     /translation="MRAKRALVTGGSGAIGAAICRVLAARGLQVIVHANNRLEAAEGL
                     AVELRAGGAKAQAVAFDVTDRAAAAKALGALVGQAPIQVLVNNAGVHDDAPLAGMRGE
                     QWDRVIDVSLNGFYNVTQPLLLPMMGTRWGRIVNISSVAGVIGNRGQVNYAAAKAGLH
                     GATKSLALELATRGITVNAVAPGIIVSPATDKVFPKEAVEAMVPMKRAGEPEEVAALV
                     AFLASDQAAYISGQVISINGGMA"
     gene            3025..4146
                     /gene="earP"
                     /locus_tag="VE030_02485"
     CDS             3025..4146
                     /gene="earP"
                     /locus_tag="VE030_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HZF22315.1"
                     /translation="MRWDLFCNVVDNLGDIGVSWRLARQLAAEHRAVVRLWADDLATF
                     RRLVPALDPEAEVQSLGGIEVRRWVTPFATVEPADVVVETFGCELPAPYVEAMAARAR
                     PPVWINLEYLSAEPWVGGCHGLPSPHPRLPLTKFFFFPGFAPGTGGVLIEKGLAARRL
                     QFQSDTDGVTRFLASLGVPPRAQGEQLISLFCYPQAMVAALLDTWATVGHPVRALVFA
                     GTAAADVLAARGLAGRGRASGLAVQVLPFLSQDDYDRVLWSCDWNFVRGEDSFVRAQL
                     AARPLVWQAYPQAGNAHRLKVEAFLTRYLEYAGHVTSANLAMLWAVWNGFAPADGLAA
                     AWTACTGPKYPELAQRWQQSLAGPGDLAGNLAKFCAERL"
     gene            4199..4759
                     /gene="efp"
                     /locus_tag="VE030_02490"
     CDS             4199..4759
                     /gene="efp"
                     /locus_tag="VE030_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HZF22316.1"
                     /translation="MRQAQEVRAGNVIMVGKDPMVVQRAEYNKGGRNSAVMKMKLRNL
                     LTGGVSEVVYKADEKLENLVLDRRELTYSYFAEPSYVFMDAEYNQYEVDKENMGDALN
                     YLEEGLPCQVVFYDDRAISVELPNSVVRKITYTEPAVRGDTSGKVLKPAKIATGFEVR
                     VPLFCAIDDKIEIDTRTGEYLRRITG"
     gene            complement(4879..5808)
                     /locus_tag="VE030_02495"
     CDS             complement(4879..5808)
                     /locus_tag="VE030_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22317.1"
                     /translation="MRTLIVISSATAVLAACASPWLEQVVALPEETLPTSGFSSLALI
                     PTDNPSFAYRALSERVPAVLSVWSTDETAVRDTCDAREPRGNNGKESPAVVLVPSANQ
                     YVLWRPALADAKVEEAMQRYARVTVQDARRPFYGDPERRNFEPAWRCFRFTRFVNRDD
                     DAPAFDFVGQLRLGDSGDYVQVRPLRLYVAPGAERHLAIGLRLEAVWRQGNVGKMEIV
                     FEPVLLAMRLKSGVEELHYFLDKYWEHYPILPVIPVSMDARGRLVQTAPATVTVTAVE
                     FRKPDQAKEALLRTARSAGDHLGALIAAALTAH"
     gene            6248..>7068
                     /locus_tag="VE030_02500"
     CDS             6248..>7068
                     /locus_tag="VE030_02500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZF22318.1"
                     /translation="MLQIGIEPDGSANWRVESRVDDLKADILMVDDEPSNLLALEGLL
                     EPLGQNLLRAQSGEEALRLVLKHNPAVILLDVRMPGMDGFETARMIRRRERSRHTPII
                     FLTGVSREMESAFRGYEVGAVDYLVKPLVPEVLRSKVSVFVDLHRKNAALSNEIRERK
                     IAEEHLRESEVQLRALAARLISVREEERTRIAREIHDELGQVLTGLKMDVMWLAKRLA
                     GTEKQLVEKTETMCSLIDSTVHLIRRISTGLRPEILDDMGLVAAIGWQAKEFQKR"
BASE COUNT         1337 a         2144 c         2296 g         1291 t
ORIGIN      
        1 cgcgccgagg atctggctgc cggcgtatcc gcctgcggcg ttgaaggccg ggcgttgcgg
       61 cgtggcgaac gccggcgcca cgccgtagaa atagttgtga tagcgcctgt caccgtagag
      121 cggcccggcg gcgaagccca cgttccagcc ttttccgagc caggtgtcgc gaaagtcgat
      181 gttgatctgc ggctggaata cccagccgac attcttcgtg tgcgaaaaat cggtcgcaat
      241 caccgtgcgc gccggcaacc gaagaccgaa ctccatgctg cggtcgggct tgcgcaggat
      301 cgtgagcagc aggttcggtc ccagctccag cgtcggatcg agatccggca tgcccctgcg
      361 ggcggcgtta tccgtgctgt ccaccggaat cgagccattt acgctcaagg acaattccac
      421 ccggtcgttc ttgaggaact gtccacgtac gctatcgcga tccgctttca ggtactcgcc
      481 gcgatagacc aggtagggga cgggtaacac gaaggtgcgg cgctgatccg agcctcgata
      541 ttccggaaag ctgatcacgg cgactccagc gccggcctcc caaagcggca gttccgccgg
      601 tcgcgcagtg cgcggcaatg ctgccgctat tgccgcggcc atggctgcga tcaacaggcc
      661 gtgcatcgaa accatcacga tcccggcgtt tgcgccatcc gctcgcgcaa tgtccgccgg
      721 cgatggcgcc aggcggcaaa actacggcgc ggatacacaa ggttcaacaa gtagtagagg
      781 ctcttgaacg cgcgcaggct caagtggatc ggcgtgccac ggaacaagtc acctgccagc
      841 aacgacagca cggcttcctg catgcgcagc ctgttgctcg ggtgcatgaa catgtcgcgc
      901 atcgcgggca gggtcatgcg gtagatgaac cacgagaagc cgccgactcc cgagcgcacg
      961 ctgcgatcga atttgcgcag cagcgccggc gcctgcggcg catcgcgcag gctggcgtcg
     1021 accgcgtcgg cgcccaaaaa tgcactgttc atggccagca tgaccccgga cgagaacacc
     1081 gggtcgatga aggcgaacgc gtcaccgatc atgatgtagc gctccccgcg catcctgtcc
     1141 gcctggtacg aaaaatttcc ggtcgcggtg acgccatggg tgagctttgc atcgcgcagg
     1201 cgttccgcca gtccaggggc catggcaatg gtgtcgagga agaaagtggt cggatcggtt
     1261 tgccttgatt tcatgtagta cggccagcac actgcaccga tgctggtggt gccgtcgagc
     1321 aatggaatga accagaacca gccgtgatcg aaccagaaca tgctgatgtt gccggcggcc
     1381 ttgcccggca gacgctgcgc gccggtgaaa tgcccgtaaa tcgccgcact gttgtgggaa
     1441 cggttgcggc gcttgatgcc gaagcggttg gcaagaaagg tgtcgcgccc cgacgcatcg
     1501 acgaagaagc gtgcgcgcca ggtcctttca ccgccgttgc tcatgcgcgc gtcgatgcgc
     1561 acgtcctgcc gggcccggaa ttcgacgttg gtgattaggc attcctcgaa cgtgcgcgcg
     1621 cctttcgcgc cgcagttgcg gaacaggatc tcgtcgaact cggagcgcct cacttccaaa
     1681 gcgttcggat aggtcttgtc ccaagcttgg ctgaagtcga aggtaaccgg cggcccatgc
     1741 tcaggggaat tgaactcggc gccgtacttc ggcatgccga tgcctttgac ctgctcatac
     1801 acgccgagtt gccgcagcag cggcatgttc aggggcagca gcgattcgcc gatgtggaag
     1861 cgcggatggc gctccttctc cgcgatcacc acgttccagc tgcgttccgc cagcagggcg
     1921 gcgatggtcg agccggccgg gccgccgccg agaacgaaca cgtcgcagtg cgaagccgcg
     1981 gattgcgcag cagccggcgc gctcacgccg gcatccccgc agcgcagaat tgcgatactg
     2041 tcgtcgacat ttccggtcag aaagcgaatt cgggtcgtgt ggcgaatatt acccgccgca
     2101 aacggcagac ccaagacaat atttccggca gaatccggca atggttgatg caatgagggc
     2161 gaaacgcgca ctggtgaccg gcggcagcgg tgcaatcggc gcggcgatct gccgcgtgct
     2221 cgcggcccgg ggcttgcagg tgatcgtgca cgccaacaac cggctggagg ctgcggaagg
     2281 gctcgccgtg gaattgcgcg cgggcggggc gaaggcgcag gcagtcgctt ttgatgtaac
     2341 cgaccgcgcc gccgccgcca aggcgctcgg cgcgctcgtt ggccaggcgc cgatccaggt
     2401 gctggtcaac aacgccggcg tgcacgatga cgcgccactc gcgggcatgc gcggtgagca
     2461 gtgggaccgt gtcatcgatg tttccctgaa cggcttctac aacgtcaccc agccattgct
     2521 gctgccgatg atgggaaccc gctggggccg tatcgtcaac atctcctcgg tggccggcgt
     2581 gatcggcaac cgcgggcagg tcaactatgc cgcggcaaag gcgggtctgc acggtgccac
     2641 caagtcgctc gcgctcgaac ttgccacgcg cggtatcacc gtcaacgcgg tcgcgccggg
     2701 catcatcgtc tcgccggcga ctgacaaggt atttccaaag gaagcggtcg aagccatggt
     2761 gccgatgaag cgtgccggcg aacccgaaga agtagcggcg ctggtggcgt ttctcgcctc
     2821 cgaccaggcc gcatacattt ccggccaagt gatctctatc aacgggggaa tggcgtgaaa
     2881 gcaggggcga ggggcgaggg acgagggacg aggggaaaca gttagcaagc attcgttttt
     2941 acatttatct tggcaaacag ttttggtgat ctggttttga cgtcccactc tcgtccctcg
     3001 tccctcgccc ctcgtcccta ggtcatgcgc tgggacctct tctgcaacgt cgtcgacaac
     3061 ctcggcgaca tcggcgttag ctggcggctg gcgcgccagc ttgctgctga acatcgcgca
     3121 gtggtgcggc tgtgggccga tgatctggcg accttcaggc ggctggtgcc tgcgctcgat
     3181 cccgaagccg aagtgcagtc gctcggcggc atcgaggtgc gacgctgggt tacgccgttt
     3241 gcgacggtgg agcctgccga cgtggtggtg gagactttcg gctgcgagct gccggctccg
     3301 tacgtcgagg ccatggccgc gcgtgcgcgg ccgcccgtgt ggatcaatct cgagtacctc
     3361 agcgcggaac cctgggttgg cggttgccac ggcctgccgt cgccgcatcc gcggctgcct
     3421 ttgacgaagt tcttcttctt ccctggcttc gcccccggta ccggcggcgt gctgatcgag
     3481 aaggggctgg ccgcgcggcg gctgcaattc cagtccgaca cggacggcgt gacgcgattt
     3541 ctggcctcgc tcggcgtgcc gccgcgggcg cagggcgagc agttgatctc gttgttctgc
     3601 tatccgcagg cgatggtcgc tgcgttgctg gatacctggg ccaccgtcgg gcacccggtg
     3661 cgcgcgcttg tgtttgccgg caccgcggcc gcggatgtgc tcgcggcgcg cggcttggcg
     3721 ggacgcgggc gcgccagcgg gcttgccgtg caagtgctgc ccttcctttc gcaggacgac
     3781 tacgaccgcg tactgtggtc ctgcgactgg aactttgtgc gtggcgagga ttccttcgtg
     3841 cgtgcgcagc tggcggcacg gccgctggtc tggcaggcct atccgcaggc gggcaacgcg
     3901 caccggctga aggtcgaagc cttcctaacc cgctatcttg agtatgcggg gcacgtcacc
     3961 agcgcgaatc tggccatgct gtgggcggtg tggaacggtt ttgcccccgc tgacggcctg
     4021 gcggcggcct ggacggcctg caccgggccg aaatatccgg aattggcgca gcgctggcaa
     4081 caaagtctcg ccggccccgg cgatctggcc gggaatctgg ctaaattttg tgcagaaagg
     4141 ctataattca acgctttttt cgcccaaacc attattagtt tatttgtcag gaaacacgat
     4201 gagacaggca caggaagtgc gcgccggcaa cgtgatcatg gtcggcaagg atccgatggt
     4261 cgtgcaacgc gctgaataca acaaaggcgg ccgcaactcg gctgtcatga agatgaagct
     4321 caggaacctg ctgaccggcg gcgtcagcga agtggtctac aaggccgacg aaaagctcga
     4381 gaacctggtg ctcgaccgga gggagctgac gtactcgtat ttcgccgagc cctcgtacgt
     4441 gttcatggat gccgagtaca accagtacga ggtggacaag gaaaacatgg gcgacgcgct
     4501 caactacctc gaggaaggcc tgccctgtca agtggtgttc tatgacgaca gggcgatctc
     4561 ggtggagctg cccaacagcg tggtgcggaa aatcacctac accgagccgg ctgtgcgcgg
     4621 cgatacctcg ggcaaggtac tcaagcccgc caaaatcgcc accggcttcg aagtgcgcgt
     4681 gccgctgttc tgcgctatcg acgacaagat cgagatcgac acccgcaccg gcgagtacct
     4741 gcgccggatc acgggctgag tccagcttag ggggtacgcc gacccggaaa tcacatttct
     4801 catcacgaag gcaccaagag caagaagaaa cacgcagagt actttgtgtt ttcgcggcga
     4861 gatcttcgat cgtcgaagtt agtgagccgt caaggctgcg gcaatcaagg cgccgagatg
     4921 gtctccggcg ctgcgtgccg tgcgcagcag cgcctccttc gcctggtcgg gtttgcgaaa
     4981 ctcgaccgcg gtcacggtca ccgtggccgg cgccgtctgc accagtcggc cgcgcgcatc
     5041 catcgatacc gggatcaccg gcaggatcgg atagtgctcc cagtacttgt ccaggaaata
     5101 gtgcaattcc tcgacgccgg atttcaatcg catcgccagc aacaccggtt cgaacacgat
     5161 ctccattttg ccgacgtttc cttgccgcca caccgcctcc agccgcaggc cgatcgcgag
     5221 gtgtcgctcc gctcccggag cgacatacag ccgcagcggg cgcacttgca cgtagtcgcc
     5281 actgtcgccg agccgcaact gtccgacgaa gtcgaatgcc ggcgcatcgt cgtcgcggtt
     5341 gacgaagcgg gtgaagcgga aacagcgcca cgccggttcg aagttgcggc gttcggggtc
     5401 gccgtagaac ggcctgcgcg cgtcctgtac ggtcacgcgg gcgtaccttt gcatcgcctc
     5461 ttccactttc gcatccgcaa gtgccgggcg ccacagcacg tactggttgg cggagggaac
     5521 gagcaccacg gccggggatt ctttgccgtt gttacctctg ggttcgcgtg cgtcgcacgt
     5581 atcccgtacc gcggtctcgt cggtgctcca caccgacagt acggctggca cgcgctcgga
     5641 gagcgcgcga taggcgaagc tcggattgtc cgtgggaatc agcgcgagcg aggagaatcc
     5701 cgacgtgggc aatgtttcct ccgggagcgc gacaacctgt tcgagccagg gagaagcgca
     5761 cgccgccagc actgccgttg ctgaggaaat cacgatcaag gtacgcataa aaaaacactg
     5821 ctccggtctg cttatgaggt cggcgaatgg actttccgga atgaggtcga gggtgcccga
     5881 ttccgaatcg ggtgcgcaac cccctgtcgg gaacgctggg gcgcaaggcc gaacacttat
     5941 ccctaaaaga ccttttgcgg tcagtgcgtt gacaaaagtc caacgttctc gatcttttca
     6001 attgcggcga ttagcggccg accgatcggc aaagccggta tggtacgcag cattaccccg
     6061 gtcacggtag caaaacgcgt cctcccacgg atcgccgatc gcttcagttc gaccggccaa
     6121 gccggatgca agacagcccg cttttatcca ccgatacttg ttggtaacct tgtcctaaac
     6181 gggggaaggg cgtttgcggt cgcgcaactt ctcatgttgc gtcataagtt gccgatcaga
     6241 ccggaacatg ctgcaaattg gcatcgaacc ggacggatcg gccaactgga gggtggaatc
     6301 tcgcgtggac gacttgaagg cggatatttt gatggtcgac gacgagccca gcaatctgct
     6361 ggcgctcgaa ggcctgctgg aacctctggg gcaaaacctg ctgcgcgcgc agtccggtga
     6421 agaggcgtta cgccttgtgc tcaagcacaa cccggccgtc attcttctcg acgtgcgcat
     6481 gcccgggatg gacggcttcg aaaccgcgcg gatgatccgc cggcgcgaac ggtcccggca
     6541 cacgccgatc atctttctta ccggcgtgtc cagggaaatg gaatccgcgt tccgcggata
     6601 cgaagtcggc gcggtggatt acctggtaaa gccgctggta ccggaggtgc tgcggtcgaa
     6661 agtgtcggta ttcgtcgatc tgcacaggaa gaacgcagcg ttgtcaaacg agatcaggga
     6721 aagaaaaatt gccgaggaac atttacggga gtcagaggtg cagttgcggg cgctagccgc
     6781 gcgcctcatc tccgttcgag aggaggaacg tacccgaata gcgcgcgaga tccacgacga
     6841 gctgggacag gtgctgaccg gtctgaagat ggacgtcatg tggctggcca agcggcttgc
     6901 cggaactgag aagcagcttg tggagaaaac cgagaccatg tgcagcctca tcgattccac
     6961 ggtgcacctc attcgaagga tttcgaccgg cctgaggccg gagattctcg acgacatggg
     7021 gctggtggcg gcaatcggct ggcaggcgaa agagttccag aaacgcat
//
LOCUS       DASQOP010000067         9044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1235189, whole genome shotgun sequence.
ACCESSION   DASQOP010000067 DASQOP010000000
VERSION     DASQOP010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9044
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235189"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..2586
                     /locus_tag="VE030_02505"
     CDS             43..2586
                     /locus_tag="VE030_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZF22319.1"
                     /translation="MKKTAFWKADWFLGLVVAVVLFVASGSDFIQSLERQAYDLGVRA
                     SSHAPSQRVAVIAIDDQSIANIGRWPWPREFHAQMINKLKQAGAKVVGYTSFFFEPQV
                     DPGLVYIHKLAELYRALPPAQQQNLADFASVLAEADDVLNSDHKLAAAVAQAGNVALP
                     MLFTIGEPRGNPDHPLTDDILKNQITRVIDRIGAAEHNIFPVPALQAVAPLGEIASGA
                     AAVGHLNALPDVDGSTRTESLVVRYYDQYFPSLSLVLAAKSLNLGPADVELRLGEGVK
                     LGKLTINTDPYLQMYTYFYKDRDGKPAFPLDSFYDVISEKIPAEKYRDKVVLIGATAA
                     GVGTYQVTPISPGMAPVVTLAHSVSSILQEDFFVAPTWGFWAERAAFLLVALYLILLL
                     PRLKAGMGFTVSALLLAALFAAHFVLMAGQLMWIQLMASAVLLVIGHVLLTTRRFLST
                     ERGKAKSDLDSAESNRMLALAFQGQGQFDMAFDKFRKVPLDDQLMDNLYNLALDFERK
                     RQFNKAQAVYEYMATYNPKFRDLDQKLNRAKQMSATVILGGGGRTNASTLVLEDGAME
                     KPMLGRYQVEKELGKGAMGVVYLGKDPKIGRIVAIKTLALSQEFEADELEEVKQRFFR
                     EAETAGRLNHPNIVTIYDAGEEHDLAYIAMEFLKGRDLMPQTKPGNLLPLPKMLSVVA
                     RVAEALAYAHRQNVVHRDIKPANIMYDAQVDSVKVTDFGIARITDSSKTKTGMVLGTP
                     SYMSPEQLAGKKIDGQSDLFSLGVTLYQLACGRLPFQGDSMTQLMFKIANEPPADILG
                     IDPTLPECLAALINKALAKEMPARFKNGEEMAQALRQCAAQLGTVEVTL"
     gene            2600..3424
                     /locus_tag="VE030_02510"
     CDS             2600..3424
                     /locus_tag="VE030_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HZF22320.1"
                     /translation="MNLTPSLEMVKATHTGMVRSHNEDSITTDPQIGLAVLADGMGGY
                     NAGEVASGIATALISSETREALARHVPHELDKSSGSPLATRLLREIIARANTSIFQSA
                     NSQPQYAGMGTTLVVTLLCNNQITVAHIGDSRCYRFRGDKLQQITRDHSLLQEQIDSG
                     LLTKEAAKRSQNKNLVTRALGIEPEVEAEIRTYPAQTGDIYLLCSDGLNDMVEDEDIE
                     MTLAAIGNNLPLAADQLVQMANDNGGRDNVSVVLIRVKKDFPADDGLWAKLLSWFK"
     gene            3437..4216
                     /locus_tag="VE030_02515"
     CDS             3437..4216
                     /locus_tag="VE030_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZF22321.1"
                     /translation="MAKLILSLDSQVIKEFTLTKERSTIGRKPHNDIQIDNLAVSGEH
                     AIIMTILNDSFLEDLGSTNGTLVNGQPVKKHFLQNNDVVEIGKYKLKYVSETAAAMSQ
                     AEFEKTMVLRAPAAGTKAETKAPAEGQNNVQAAKLSDTVINAQAAPAVAEASNPAPSP
                     VAAAPAATAMPGGQGIIQILSGPNAGKELPLLKALTTLGKPGVQVAVIAKRPHGYFIT
                     HVEGANFPVVNGKALDAQAHQLNDHDIIELAGVKMEFFLKS"
     gene            4330..6558
                     /locus_tag="VE030_02520"
     CDS             4330..6558
                     /locus_tag="VE030_02520"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZF22322.1"
                     /translation="MKRHLARIALGLVVVLIFVGHAARYYQIPLINRFENIVYDARLR
                     LTTRNSLDPRLVIVDIDEKSLSAEGRWPWRRDKLALMLDQLFDHYKVGIVGFDVVFAE
                     RDESSDLGVLRRLGENQLRGNSQYQAALKEIGPQLEYDRVFAEKLKNRAVVLGYYFSD
                     VASEDGKGRTTGALPKPVLPAGTFKGRNIGFTRWVGYGANLAEFQEAAASGGHFNPLP
                     DEDGVTRRVPMLAEYEGAYYESLSMAVVRLGLGSPPVIAGFPEEKLFTKNYPGLEWID
                     IGSVRIPVDVQVAALVPYRGRQGSFKYVSATDVLHGTAPIDDLRNKIILVGTTAPGLF
                     DLRATPVASVYPGVEIHANLITGMLDGNIKQKPPYVLGADVVLLLLSGLALAMALPLA
                     NPLRATVLTLVLLAAVFASNVLVWIGGNLVLPLASGLLMIAMLFALNMSYGFFVESRA
                     KRQITGRFGQYVPPELVEEMSRDPESFSMEGESRELSVLFTDVRGFTTISEGLDPKQL
                     SRLMNEFLTPLTRVIYKHRGTIDKYMGDCIMAFWGAPLGDPQHARSAVLAGLEMYQAL
                     EALQPHFKKMGWPPIHIGVGVNSGRMSVGNMGSEIRLAYTVMGDAVNLASRLEGITKE
                     YRVNMIVGENTRAQVPDVVFRELDRVKVKGKDEPVTIYEPIGLAMEVQTAVQDELRLW
                     GQALKLYRARDWDMAELQLLNLQKLNAASGFYKLFLGRIALLRSNPPEQGWDGSWKFE
                     TK"
     gene            6561..7325
                     /locus_tag="VE030_02525"
     CDS             6561..7325
                     /locus_tag="VE030_02525"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HZF22323.1"
                     /translation="MQIRVLGCSGGIGGNLRTTTMLLDYDVLIDAGTGVGDLSLTELL
                     KIDHVFITHSHLDHITSLPFLVDTVGPMRDRPITVHCTEPTHKILQDHIFNWKIWPDF
                     AEIPSKSAPYLKYATFAVGETVDLGGRRITVLPANHVVPAVGFRLDSGEASLVFTGDT
                     TINDALWTEVNKIENLRYLIIETAFPNEEKELAVASKHLCPSMLAEELAKLKRPTRVY
                     ITHLKPGAGTLTMQEVEECAERYNPRMLSNGQVFKF"
     gene            7422..>9044
                     /locus_tag="VE030_02530"
     CDS             7422..>9044
                     /locus_tag="VE030_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HZF22324.1"
                     /translation="MSTVFETKPQSGHNLAEMSHKLEFQKRLQAVTNKIHATSNIDEI
                     MLQLSQDICALFEADRLTIYVVSEDRTSIVSKVKTGLASFKDLKLPVNEQSIAGFVAL
                     SKKLINIRDVYDDNELRGHNAQLKFLKEVDRRTGYRTKQMLVVPVTDGPGGELIGVVQ
                     VINAKSGQPFSTVAEEGAAHLGETLAIALKQRSRPTAMLRGKYDHLVTNAVLSAEEME
                     LATRSARRKNLDIEDVLVDEFQVKAPAIGEALSQFFRVPYESFKQDRVKPMDLLKNLK
                     RDFVESSHWVPIEDVREGLIVLTTDPEKVRASKVVGNIFPKHKVVFKVCTRREFSATL
                     DQFFGAEAGGDTSNISDLLSTMDDEGDDSAAVATDDASLAQDNELVKLVNKIIVDAYH
                     QGASDIHIEPYPGKGKTEVRFRKDGTLQPYISVPASYRNAIAARLKIMCDLDISEKRK
                     PQDGKIKFKKFGPLDIELRVATIPSAGGVEDIVMRILSAGEPIPLDKLVLSEHNLGTL
                     KNVVSKPYGLFFVCGPTGSGKTTTLHSVLGYLN"
BASE COUNT         1930 a         2739 c         2736 g         1639 t
ORIGIN      
        1 actagaaatc aaagccttca ggcatccaac agggtgggtg gcatgaagaa gacggctttc
       61 tggaaagccg attggttcct cggattggtc gttgccgttg ttttgttcgt cgcgtcgggc
      121 agcgatttca ttcaaagtct cgagcgtcag gcctacgacc tcggcgtgcg tgcctccagc
      181 cacgccccct cgcagcgcgt cgcggtcatc gccatcgatg atcagagcat cgccaacatc
      241 gggcgatggc cttggccgcg ggagttccac gcgcagatga tcaacaagct caaacaggcc
      301 ggggccaagg tcgttggcta cacctcgttc ttcttcgagc cgcaggtcga tcccgggctc
      361 gtctatatcc acaaactcgc agagctctac cgggcactcc cgcccgcgca gcagcagaac
      421 ctcgccgatt tcgcctcggt gctggccgag gcggacgatg tactcaacag cgaccataag
      481 cttgccgccg cggtggcgca ggccggaaac gtggcgttgc caatgctttt caccatcggc
      541 gaaccgcgcg gcaatcccga ccatccgctt accgacgaca tcctgaagaa ccagatcacc
      601 agagtgatcg accgcatcgg cgcggccgaa cataacattt tcccggtgcc ggctctgcaa
      661 gccgttgcgc cgctcggaga aatcgcgtcc ggtgccgcag cggtcggcca cctgaatgcg
      721 cttccggatg tcgacggcag cacgcgcacc gaatcgctgg tggtgcgcta ctacgatcag
      781 tacttcccgt cgctttcgct ggttcttgcc gccaagagcc tgaatctggg accggcagac
      841 gtcgaactgc gcctcggcga gggggtgaag ctggggaagc tgacaatcaa taccgatccc
      901 tacctgcaga tgtacaccta cttttacaag gatagggacg gcaaaccggc atttccgctg
      961 gattcctttt acgacgtaat ctcggaaaaa atcccggcgg agaaataccg ggacaaagtg
     1021 gtgttgatcg gcgcgaccgc tgccggcgtc ggaacctacc aggtgacgcc gatttcgccc
     1081 ggaatggcgc cggttgttac tctggcgcac tcggtgtcga gcattctcca ggaggatttc
     1141 tttgtcgcgc ccacctgggg tttctgggcg gaaagggcgg ctttcctgct agttgccctg
     1201 tatctgatcc tgctgctgcc gcggctcaag gcaggcatgg gttttaccgt gagcgcgctg
     1261 ttgcttgcgg cactgtttgc cgcgcatttc gtgctgatgg cgggccagct gatgtggatc
     1321 cagctgatgg cctcggcggt gctgctggtc atcggccacg tcctgctcac caccaggcgt
     1381 tttctctcca ccgaacgcgg caaggccaag tccgacctcg attctgctga atccaaccgc
     1441 atgctggccc tggccttcca ggggcagggc cagttcgaca tggccttcga caagttcagg
     1501 aaggtgccgc tcgacgacca gctgatggac aacctataca accttgcgct cgatttcgag
     1561 cgcaagcgcc agttcaacaa ggcccaggcg gtgtatgagt acatggccac ctacaatccc
     1621 aagttccgcg acttggatca gaagctcaat cgcgccaagc agatgtccgc gaccgtgatc
     1681 cttggcggtg gcgggcgcac caacgccagc acgctggtcc tcgaggatgg tgcaatggaa
     1741 aagccgatgc tcggccgtta tcaggtggaa aaggaactcg gcaagggcgc gatgggcgtt
     1801 gtttatctgg ggaaagaccc taagatcggc cgcatcgtcg cgatcaagac gctggcgctg
     1861 tcacaggagt tcgaagccga cgagctggaa gaggtgaaac agcgcttctt ccgtgaagcc
     1921 gagactgccg gccggctcaa tcatcccaat atcgtcacca tctacgatgc cggcgaagag
     1981 cacgacctgg cctacattgc catggaattc ctcaagggca gggatctgat gccacagacc
     2041 aaaccgggca acctgctgcc cttgcccaag atgctgtccg tggtcgcgcg cgtcgccgaa
     2101 gccctggcct acgcccaccg gcagaacgtg gttcatcgcg acatcaagcc ggccaacatc
     2161 atgtatgacg cgcaggtcga ttcggtcaag gtcaccgact tcggcattgc gcgcatcacc
     2221 gattcgtcca agaccaagac cggcatggtg ctgggcacgc cctcgtacat gtcgcctgaa
     2281 caactggccg gcaaaaagat cgacggccag tccgatctat tttcgctggg tgtgacactg
     2341 tatcagcttg cctgcggcag gttgccgttc cagggcgatt cgatgaccca attgatgttc
     2401 aagatcgcca acgaacctcc cgccgacata cttggcatcg acccgacgct acccgagtgt
     2461 ctggcggcgc tcatcaacaa ggcgctcgcc aaagagatgc ccgcgcgctt caagaatggt
     2521 gaagaaatgg cgcaggcgct acgccagtgc gcggcgcagc ttggaaccgt ggaagtgacc
     2581 ttgtgatgga ttggaagaaa tgaatctgac tccctccctg gagatggtga aagccaccca
     2641 caccgggatg gtgcgctctc acaacgagga cagcattacc accgacccgc agatcgggct
     2701 ggcggtgctg gccgacggca tgggtggcta caatgccggc gaagtcgcaa gcggcatcgc
     2761 cactgcgctg atttctagcg aaacccgcga agcgctcgcc cggcatgtgc cgcacgaact
     2821 ggacaagagt tccggttctc ctctggcgac ccgcctgctt cgggagatca tcgccagggc
     2881 caacacctcg atcttccagt cggccaacag ccagccgcag tacgcgggca tggggaccac
     2941 gctggtagtg accttgttgt gcaacaacca gatcacggtt gcccatatcg gcgattcccg
     3001 ctgttaccgc tttcgcgggg acaagctgca gcagataacc cgcgaccatt ccctgctgca
     3061 ggaacagatc gacagcggcc tgctcaccaa ggaggccgcc aagcgctcgc agaacaagaa
     3121 cctggtgacg agggcgttgg gcatcgagcc ggaagtcgaa gcggagatcc gtacctatcc
     3181 ggcccagacc ggcgacattt atctgctgtg ctccgacgga ctcaacgaca tggtcgagga
     3241 cgaggacatc gagatgacgc tggcggcgat cggcaacaac ctcccgctcg cggccgatca
     3301 actggtacag atggcgaatg acaacggcgg gcgcgataat gtctccgtgg tgctgatcag
     3361 ggtgaagaag gacttcccgg ccgacgacgg cctgtgggcg aagctccttt cgtggttcaa
     3421 ataagaggaa gcagatatgg cgaagctgat actcagtctc gatagccagg taatcaagga
     3481 gttcaccctc accaaggagc gatccaccat cggccgcaag ccgcataacg acattcagat
     3541 cgacaatctg gccgtcagcg gcgagcacgc catcatcatg accatcctca acgattcctt
     3601 tctcgaggat ctgggcagca ccaacggcac gctggtcaac ggccagccgg tgaagaagca
     3661 cttcctccag aacaacgacg tcgtcgaaat cggcaaatac aagctcaagt acgtcagcga
     3721 aacggcggcc gccatgagcc aggcagagtt cgaaaagacc atggtgcttc gcgctcccgc
     3781 cgcaggaacc aaggcagaaa caaaggcgcc ggccgagggc cagaacaacg tgcaggctgc
     3841 gaagctgagc gataccgtca tcaatgccca ggccgcgccc gcggtggcgg aagcgtccaa
     3901 tccggctccg tcgccagtgg ccgccgcgcc tgccgccacg gcaatgccag gtggacaagg
     3961 catcatccag attctctccg gacccaacgc cggcaaggag ctgccgctcc tcaaggcgct
     4021 gaccacgctg ggcaagcccg gggtgcaagt ggcggtcatc gccaaacggc cacacggcta
     4081 cttcatcacc cacgtggaag gcgccaactt cccggtggtc aacggcaagg cgctcgatgc
     4141 ccaggcccac cagctcaacg atcacgacat catcgagctg gccggggtaa agatggagtt
     4201 cttcctgaaa tcctgagccg aaccacggcc cgcacggacc tttctgcacg cgccagagca
     4261 gacgacgata accaggcggc cccaccgagg cggcgctgat ccggcgcgcg gcgagtcgag
     4321 ggagacagct tgaagaggca tctggcgcgc atcgcgctcg ggctggtggt ggttctgatt
     4381 tttgtcggcc acgccgcccg ttactaccaa atccccttga tcaatcgctt cgagaacatc
     4441 gtttatgacg cgcgcctgcg cctgacgacg cgtaacagcc tcgatccgcg cttggtcatc
     4501 gtcgacatcg acgagaaaag cctctccgcg gagggccgtt ggccctggcg ccgcgacaag
     4561 ctcgcgctga tgctcgatca gctgttcgat cactacaaag tgggtatcgt tggcttcgac
     4621 gtagtgtttg cagaacgcga cgaaagttct gatctggggg tgcttaggcg gctcggcgag
     4681 aaccagctca ggggcaactc gcagtaccaa gcggcgctca aggagatcgg tccgcaactc
     4741 gaatacgacc gcgtgttcgc ggagaagctg aagaatcggg cggtggtgct cggctattac
     4801 ttttcagatg tcgcatcgga agacggaaaa gggcgcacca ccggtgcgtt gcccaagccg
     4861 gtcctgccgg ccggcacttt caagggcagg aatatcggat ttacccgctg ggtcggttac
     4921 ggcgccaatc tcgcggaatt ccaggaagcc gcggcaagcg gcggtcactt caaccccttg
     4981 cccgacgaag acggtgtgac caggcgggtg ccgatgctgg cggagtacga gggcgcgtat
     5041 tacgaatcgc tgtcgatggc ggtggtgcgc ctcgggctgg gttcgccgcc ggtcattgcc
     5101 gggtttccgg aggaaaagct cttcaccaag aattatcccg gcctggagtg gatcgacatc
     5161 ggctcggtgc gcattccggt ggatgtccag gtggcggccc tggtgcccta ccgcggccgc
     5221 cagggcagtt tcaagtatgt ttcggcgacc gatgtgttgc atggcacggc gccgatcgac
     5281 gatctgagga acaagatcat cctggtgggc acgactgcgc cggggctgtt cgacctcagg
     5341 gcgactccgg tggcaagcgt gtatcccgga gtggagatcc acgccaatct gatcaccggc
     5401 atgctcgacg gcaacatcaa gcagaagccg ccatacgtgc ttggcgccga cgtggtgctg
     5461 ctcctgttgt cgggtctggc gctggcgatg gcgctgcctc tggcgaatcc tttgcgcgcc
     5521 accgtgctca cactggtgct gctggcggcg gtgttcgcca gcaatgtgct ggtgtggatc
     5581 gggggcaacc tggtattgcc tctggcctcg ggcttgttga tgatcgcgat gctgttcgcg
     5641 ctcaacatgt cctacggctt cttcgtcgag tcgcgcgcca agcgccagat caccgggagg
     5701 ttcggccagt atgtgccgcc cgaactggtg gaagaaatga gcagggatcc cgagtccttc
     5761 tctatggaag gcgaaagccg cgagctgtcg gtgttgttca ccgacgtgcg cgggttcact
     5821 accatttccg aaggcctcga cccgaaacag ctctccaggc tgatgaacga attcctcacg
     5881 ccgctcactc gggtgatcta caagcatcgc ggcaccatag acaagtacat gggggattgc
     5941 atcatggcat tctggggagc tccgctcggg gatccgcagc atgcgcgcag tgccgtgctc
     6001 gccggactgg agatgtacca ggcgctcgag gctctgcagc cgcacttcaa gaaaatgggc
     6061 tggccgccaa tccacatcgg cgtgggcgtg aacagcggcc gcatgagcgt aggcaacatg
     6121 ggctcggaaa tccgtctggc ctacaccgtg atgggtgacg ccgtgaacct ggcttcgcgc
     6181 ctggagggca tcaccaagga atacagggtc aacatgatcg tcggcgaaaa cacccgcgcg
     6241 caggttcccg acgtggtctt tcgcgagctt gaccgggtga aagtgaaggg caaggacgag
     6301 ccggtgacca tttatgaacc gatcgggttg gcgatggaag tccaaacggc ggtgcaggac
     6361 gaattgaggc tgtgggggca ggcgctcaag ctttatcggg cgcgcgactg ggatatggca
     6421 gaattgcagt tgctcaacct gcaaaagctc aatgcagcga gcggatttta taagctcttc
     6481 ctcgggcgca ttgccttgtt gcgcagcaat cctccggagc aaggctggga cggctcgtgg
     6541 aaattcgaga ctaaatagca atgcagatca gggtactcgg ttgcagtggc ggaatcggtg
     6601 gaaacctgcg caccaccacg atgctgctcg actacgacgt gctgatcgat gccggaaccg
     6661 gggtggggga tctgagcctg accgagctgc tgaagatcga ccatgtcttc attactcact
     6721 cgcatctcga tcacatcaca tcgctgccgt tcctggtgga caccgttggc ccgatgcgcg
     6781 atagaccgat caccgtgcac tgcaccgagc cgacgcacaa gatcctgcag gaccacattt
     6841 tcaactggaa gatatggccg gatttcgccg aaataccgag caagtccgcg ccgtacctga
     6901 aatacgccac gtttgcagtg ggcgaaaccg tcgatctcgg cgggcgccgg atcaccgtgt
     6961 tgccggccaa tcacgtggtc cccgcggtcg ggttccgcct cgacagcggc gaggcaagcc
     7021 tggttttcac cggcgacacc accattaacg acgcgctttg gacagaagta aacaagatcg
     7081 agaacctgcg ctatctgatc atcgagacgg ccttcccgaa cgaggaaaag gaactggcgg
     7141 tcgcatcgaa acatctgtgc ccgagcatgc tggcggagga actggctaaa cttaagcgcc
     7201 cgacgcgggt ctacatcacg cacctgaagc ccggtgccgg cacgttgacg atgcaggaag
     7261 tcgaggagtg tgcggaacgc tacaacccac gcatgctgtc caatggccag gttttcaagt
     7321 tttaatgccc cgctggcggc aaaggcactg attagcggcc cggaagttgc tggggtagta
     7381 tccccgagtg gcacccggca aacggaagaa ggatggacgc catgagcaca gtctttgaaa
     7441 ccaagccgca aagcggccat aacctggccg aaatgagcca taaactggag tttcagaaga
     7501 ggctccaggc ggttacgaac aagattcacg cgaccagcaa tatcgacgag atcatgctgc
     7561 agctgtcgca ggatatctgc gcgctgttcg aagccgaccg gctcaccatc tacgtggttt
     7621 ccgaggacag gacgtcgatc gtctccaagg tcaagaccgg attggcttcc ttcaaggatc
     7681 tgaagctgcc ggtcaacgag cagagcatcg ccggcttcgt cgcattgtcg aagaagctca
     7741 tcaacatccg cgatgtctac gacgacaacg agctgcgcgg ccacaacgcg caactcaagt
     7801 tcctcaagga agtggaccgc cgcaccggat accgcaccaa gcagatgctg gtggtgccgg
     7861 taacggacgg ccctggcggc gagttgatcg gcgtggttca ggtgatcaac gccaagagcg
     7921 gccagccttt ttccaccgtc gccgaggaag gcgcggccca tctcggcgaa accctggcga
     7981 ttgcactgaa gcagcgcagc cgcccgacgg cgatgctgcg cggaaagtac gaccatctgg
     8041 tcaccaacgc cgtgctttca gcagaagaaa tggaactggc gacccgttcc gccaggcgca
     8101 agaacctcga catcgaggat gttctggtcg acgagttcca ggtcaaagca ccggcgatcg
     8161 gcgaagcgct gtcccagttc ttccgcgtgc cctacgaatc gttcaagcag gaccgggtca
     8221 agcccatgga cctgctcaaa aacctgaagc gcgatttcgt cgagagcagc cactgggtgc
     8281 cgatcgaaga cgtaagggaa ggcctgatcg tgcttaccac cgacccggag aaagtgcgcg
     8341 cctcgaaagt ggtgggcaat atctttccca agcacaaggt ggtcttcaaa gtctgcaccc
     8401 ggcgcgaatt cagcgccacg ctcgaccagt tcttcggcgc ggaagccggg ggcgacacct
     8461 cgaacatcag cgacctgctc tccaccatgg acgacgaggg cgacgactcg gccgcagtcg
     8521 caaccgacga cgcatccctg gcgcaggaca atgaactggt gaaactggtg aacaagatca
     8581 tcgtggatgc ctatcaccag ggggcttccg acattcacat cgagccctat ccgggaaagg
     8641 gcaaaaccga ggtgcgtttc cgcaaggacg gcacgctgca accttacata tcggtgccgg
     8701 cgagctaccg caatgccatc gccgcgcgcc tgaaaatcat gtgcgatctc gacatctcgg
     8761 agaaacgcaa accccaggac ggcaagatca agttcaagaa attcggccca ctcgacatcg
     8821 aactgcgggt ggcgaccatt ccttccgccg gtggtgtcga agacatcgtg atgcgtattc
     8881 tctccgcggg cgagccgatc cccctcgata aactggtact gtctgaacac aacctcggca
     8941 cgctaaaaaa cgtggtcagc aagccttacg ggctgttctt cgtgtgcggc ccgaccggtt
     9001 caggcaagac caccacgctg cattcggtcc tcggctatct gaac
//
LOCUS       DASQOP010000068         7995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2445259, whole genome shotgun sequence.
ACCESSION   DASQOP010000068 DASQOP010000000
VERSION     DASQOP010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7995
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2445259"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..824
                     /gene="prpB"
                     /locus_tag="VE030_02535"
     CDS             3..824
                     /gene="prpB"
                     /locus_tag="VE030_02535"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HZF22325.1"
                     /translation="MGTINAYHARLAERTGYKATYLSGGGVAAGSLGLPDLGITTLDD
                     VLTDVRRITDACGLPLLVDADTGFGSAFNIARTVKSLIKFGAGAMHIEDQVQAKRCGH
                     RPGKEIVSRDEMVDRIKAAVDARTDPAFVIMARTDALAVEGLDAAIERARACVEAGAD
                     MIFPEAMTELSMYRKFADAVKVPVLANITEFGATPLFTVQELRSANVSLVLYPLSAFR
                     AMNKAALKIYETVRREGTQRNVIDIMQTRNELYDYLDYHRFESKLDQLFAKEKGK"
     gene            821..1975
                     /gene="prpC"
                     /locus_tag="VE030_02540"
     CDS             821..1975
                     /gene="prpC"
                     /locus_tag="VE030_02540"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HZF22326.1"
                     /translation="MSEATQFTPKPKKSVALSGVPAGNTALCTVGRTGNDLHYRGYDI
                     LDFAEEAEFEEIAYLLVHGKLPTVAELAAYKRKLKALRGLPASVQAILECIPANTHPM
                     DVLRTGCSALGAVLPEHPDHKLTEARDIADRMMASFGSMLLYWYHFSHSGRRIEVETD
                     DDSIGGHFLHLLHGKKPSALWERAMHTSLILYAEHEFNASTFTARVIAGTGSDMYSAV
                     GGAIGALKGPKHGGANEFAFDTIGRYDNPDDAEADIVRRVAEKQIIIGFGHPVYTTGD
                     PRNQVIKEVARRLSKDAGDMKLFDISERVEQVMWREKKMFPNLDWYSATSYHMMGVPT
                     AMFTPIFVISRTSGWTAHVIEQRIDGKIIRPSANYTGPENLTWVPIGKRK"
     gene            2174..3616
                     /locus_tag="VE030_02545"
     CDS             2174..3616
                     /locus_tag="VE030_02545"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HZF22327.1"
                     /translation="MSAPISNVRPKPDKVLVDIADYVTKYKIKSRKAYETARYCLMDT
                     LGCGFEALEYPACTKLLGPVVHGTVVPNGAKVPGTQFQLDPVQAAFNIGATIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVAFGKKPLTVQDVLTAMIKAHEIQGCLAL
                     ENSFNKVGLDHVVLVKVATTAVVAGILGLSREEIINAISLAWVDGQSLRTYRHAPNTG
                     SRKSWAAGDATSRAVRLALIARTGEMGYPSVLTAKTWGFYDVLFKGKEFKFQRPYGSY
                     VMENVLFKISFPAEFHAQTAVECAMQLHPQLKDRLDDIKRITIRTHEAAIRIIDKKGP
                     LSNPADRDHCIQYMLAVPMIFGRLTAADYEDDVAADPRIDKLRDKMICVEDKQFTRDY
                     HDPKKRSIANGITVEFKDGSRLKEVVVEYPIGHKRRRKEGMPVLVEKFKTNLARRFPP
                     KQQKTVLELCLDPRQLAATPVNEFVDMMVI"
     gene            3623..4045
                     /locus_tag="VE030_02550"
     CDS             3623..4045
                     /locus_tag="VE030_02550"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HZF22328.1"
                     /translation="MRPAFALFFGVALSVPAQADSNPMQPGTWEFTRSGGMAANLIGR
                     FCVTAADIADPMSPVNGFLARDEKKQCSNWKIEWHAGHAQYSGTCRWKDQLVEVSGRI
                     VAGADTYSDTQDQKKEGSAPRSPILDIINGLRLAPCPD"
     gene            4069..6915
                     /locus_tag="VE030_02555"
     CDS             4069..6915
                     /locus_tag="VE030_02555"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF009520.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HZF22329.1"
                     /translation="MSHNLFNTLQEFSPAPGKKAKFYSLPALEKAGIGKISRLPVSLR
                     IVLEAVLRNVDGKKVAEEHVRQLAGWRPNAPRTEEIPFVVARIVLQDFTGVPLLADLA
                     AMRSVAARMGGNPKLIEPLVPVDLVVDHSVQVDHYGSKEALDLNMKLEFQRNKERYQF
                     MKWGMQAFDTFKVVPPGVGIVHQVNLEYLARGVHARDGIYYPDTLVGTDSHTTMINGI
                     GVVGWGVGGIEAEAGMLGQPVYFLTPDVVGVNLRGALRGGVTATDLVLTITEMLRRAK
                     VVGKFVEFFGEGVENLSLPDRATVANMAPEYGATMGFFPVDDATIEYFKGTGRTPEEI
                     EAFKSYFKAQGLYGIPRKGQIDYSQALELNLSGVAPSLAGPKRPQDRIEIGKVKNAFS
                     TLFYKPAAENGYNRPAGALERRYDSWFRGSGTMSGGGTSNSATIAEGAPAHVVEMVNN
                     RPTPDVVNDAHLGAPVRITLGHGDVLIAAITSCTNTSNPGVLLAAGLLAKKAVERGLS
                     VKKHVKTSLAPGSRVVTEYLTKSGLLPYLEKLGFYLAGYGCTTCIGNSGPLDPGIEET
                     IVKNDLVGSAVLSGNRNFEARIHANIKANFLASPPLVVAYAIAGTVLKDLMSEPLGKD
                     AKGEDVWIGDIWPSSQEIYDCMKYAMDAKTFRRLYSNPGKDNPLWQGVPSASGQVYNW
                     PKSTYIAEPPFFRDFSMQPGRTGNVFGARVLGIFGDSVTTDHISPAGSISENSPAGKY
                     LLANGVAKADFNSYGARRGNHEVMMRGTFANVRIKNLMLPPRPDGSRVEGGVTLFQPS
                     GEQMSIYDAAMKYIAQGTPTVVFAGEEYGTGSSRDWAAKGTQLLGVKAVIAKSFERIH
                     RSNLVGMGVLPLQFKGSDSVELLGIKGDDEFDILGVEGELRPQQDLKLVVKRTDGSRA
                     EVKVLLRIDTPIEVDYYRHGGILPYVLRGLVAAA"
     gene            7363..7779
                     /locus_tag="VE030_02560"
     CDS             7363..7779
                     /locus_tag="VE030_02560"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HZF22330.1"
                     /translation="MRIVLDTNVWLDWLVFNDPAVIPLKSARSDGLIRIAINSAGLEE
                     LGRVLAYAEFKLDAAARSALFTQVRNCTYRVDTVLISPLPRCADPNDQKFLELARDAQ
                     ADWLITKDKALLRLAKKQFSAVAFRIGTPEQWLASR"
     gene            complement(7816..>7995)
                     /locus_tag="VE030_02565"
     CDS             complement(7816..>7995)
                     /locus_tag="VE030_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HZF22331.1"
                     /translation="SIAQAALASYPDLIRSRFGLPANRKLVCGISFGYADAKHPIHRY
                     RTERAPVAEVATWIE"
BASE COUNT         1605 a         2534 c         2443 g         1413 t
ORIGIN      
        1 tgatcggcac gatcaacgct taccatgccc ggctcgccga gcgcaccggc tataaggcga
       61 cctacctgtc cggcggcggc gtcgctgccg gttcgctggg cctgcccgac ctgggcatta
      121 ccacactgga cgacgtgctc accgatgtgc gccgcatcac cgacgcctgc ggcctgcccc
      181 tgctcgtgga tgcggacaca ggtttcggca gcgccttcaa tatcgctcgc accgtaaagt
      241 ctctcatcaa gttcggcgcc ggcgcgatgc acatcgagga ccaggtccag gccaagcgct
      301 gcggtcaccg gccgggcaag gaaatcgtct cccgcgacga gatggtggac cgcatcaagg
      361 cggcggtgga cgcgcgcacc gatccagcct ttgtcatcat ggcccgcacc gatgcgctcg
      421 cggtcgaggg cctcgacgcc gcgatcgagc gcgcccgggc ctgcgtggaa gccggcgccg
      481 acatgatctt cccggaggcg atgacggaac tttcgatgta ccggaagttc gccgacgcgg
      541 tgaaggttcc ggtgctcgcc aacatcaccg agttcggcgc cacgccgtta ttcaccgtgc
      601 aggaattgcg cagcgcaaat gtctcgctgg tgctttatcc gctgtcggcg ttccgcgcga
      661 tgaacaaggc ggcgctcaaa atctacgaga ccgtccgccg cgagggtacg cagaggaacg
      721 tgattgatat catgcagacc cgcaacgagc tttacgatta cctcgactac caccggttcg
      781 agtcgaaact ggaccaactt ttcgcgaagg aaaagggaaa atgagcgaag cgacccaatt
      841 cacgcccaag ccgaagaagt cggtagcgct ctccggcgtc ccggccggca acaccgcgct
      901 gtgcaccgtc ggtcgcaccg gcaacgacct ccactatcgc ggttacgaca tcctcgattt
      961 cgccgaggaa gccgagttcg aggagatcgc ctacctcctg gtgcacggca agctgccgac
     1021 cgttgccgaa ctggcggctt acaaacgcaa gctcaaggcg ctgcgcggcc tgccggcatc
     1081 ggttcaggcc atcctcgagt gcatcccggc aaacacgcac cccatggacg tgctgcgaac
     1141 cggctgttcc gctctgggcg ccgtgcttcc agagcacccg gaccacaagc tcaccgaagc
     1201 gcgcgacatt gccgaccgga tgatggccag cttcggttcg atgctgctct actggtatca
     1261 cttctcgcac tctgggcggc gcatcgaagt cgagaccgat gacgattcga tcggcggtca
     1321 cttcctgcac ctgctgcacg gcaagaagcc gtcggcgctg tgggaacggg cgatgcatac
     1381 ctcgctgatc ctctatgccg aacacgaatt caacgcgtca acgttcaccg cgcgcgtcat
     1441 cgccggcacg ggctccgaca tgtattcggc ggtaggcggt gcgattggcg cgctcaaggg
     1501 gccgaagcac ggcggcgcca atgagttcgc cttcgacacc atcgggcgct acgacaaccc
     1561 ggacgatgcc gaagccgaca tcgtcaggcg cgttgccgag aagcagatca tcatcggctt
     1621 cggccatccg gtgtacacca ccggcgaccc gcgcaaccag gtgatcaagg aagtcgcgcg
     1681 gcggctctcc aaggacgctg gcgacatgaa gctcttcgat atctccgagc gggtcgaaca
     1741 ggtcatgtgg cgcgagaaga agatgtttcc gaacctggac tggtacagcg ccaccagcta
     1801 tcacatgatg ggggtgccga ccgcgatgtt cacgccgatc ttcgtcatca gccgcacttc
     1861 cggctggacc gcccacgtga tcgagcagcg catcgacggc aagatcatcc gtccgagcgc
     1921 caactacacc ggccccgaga acctcacgtg ggttcctatc ggaaaacgca aatagaactt
     1981 tttcaccgca aagacgcaaa gagcacagag gaaaaaggtc gaacaatatg gcgggcacaa
     2041 cagattggca gctccccgca gaatatttgg ccgccggtgt tgatgtcgct cttgatatcc
     2101 cattcgtttt tcttcgcgtc ctttgcgtct ttgcggtgac aaggcctgaa cgattttgag
     2161 tcctaggaac aacatgtcag cacccatttc caacgtccgc cccaaacccg acaaggtcct
     2221 ggtcgacatc gccgactacg tgaccaaata caagatcaag agccggaagg cgtacgaaac
     2281 cgcgcgctac tgcctgatgg atacgctggg ctgcggcttc gaggcgctcg agtatcccgc
     2341 ctgcaccaag ctgctggggc cggtcgtcca cggcaccgtc gttcccaacg gcgccaaggt
     2401 gcctggaacc cagttccagc tcgacccggt gcaagctgcg ttcaacatcg gcgcgacgat
     2461 ccgctggctg gacttcaacg acacctggct ggccgcggaa tgggggcatc cgtccgacaa
     2521 cctgggcggt attcttgcga ctgccgattg gctgtcgcgt aacgcagttg ctttcggcaa
     2581 gaagccgctc acggtgcagg acgtgctgac tgcgatgatc aaggcgcatg agatccaggg
     2641 ctgcctggcg ctggaaaaca gcttcaacaa agtcggcctc gaccacgtgg tgctggtcaa
     2701 agtggccacc accgcggtgg tggcgggcat tctcggcctg tccagggaag aaatcatcaa
     2761 cgcaatctcg ctggcgtggg tggacggcca gagtctgcgc acctatcgcc atgcccccaa
     2821 caccggttcg cgcaagagct gggcggcggg agatgccacc agccgcgcgg tcaggctggc
     2881 gctgatcgcc agaaccggcg agatgggcta tccgtcggtc ctgacagcaa agacctgggg
     2941 tttctacgac gtgctgttca agggcaagga attcaagttc cagcgcccct acggttcgta
     3001 cgtcatggaa aacgtgctgt tcaagatctc cttccctgcg gaattccatg cgcagaccgc
     3061 ggtcgaatgt gccatgcagc tgcatccgca gttgaaagat cgtctcgacg acatcaagag
     3121 aatcaccatc cgcacgcacg aagcggcgat ccgcatcatc gacaagaagg gccccctgag
     3181 caacccggcc gaccgcgacc attgcatcca gtacatgctg gcggttccga tgatcttcgg
     3241 tcgcctgacg gccgccgact acgaagacga tgtggcggcc gatccgcgca tcgacaagct
     3301 gcgcgacaaa atgatctgcg tcgaggacaa gcagttcacc cgcgattacc acgatccgaa
     3361 gaagcgctcg atcgccaacg gcatcacggt cgagttcaag gacggaagcc gcctcaagga
     3421 agtagtggtc gaatacccga tcggccacaa gcgccgccgc aaggagggca tgccggtcct
     3481 ggtcgagaaa ttcaagacca acctggcgcg acgtttcccg ccgaagcagc agaagacagt
     3541 gctggagctc tgcctggatc ccaggcagct cgccgccacg ccggtcaacg aattcgtgga
     3601 tatgatggtt atctgatccc tgatgcgacc cgcattcgct cttttctttg gagtcgcgct
     3661 ctccgtcccg gcgcaggcgg actccaatcc catgcaaccc ggcacctggg aattcacgcg
     3721 ctcgggcggc atggccgcga acctgatcgg ccgcttctgc gtcaccgcag cggacattgc
     3781 cgacccgatg tcgcccgtca acggcttcct cgcgcgcgac gagaagaagc aatgcagcaa
     3841 ctggaagatc gagtggcacg cgggccacgc gcaatattcc gggacatgcc gctggaagga
     3901 ccagctggtc gaggtcagcg gccgcatcgt tgccggcgcg gacacctact ccgatacgca
     3961 ggaccagaag aaagaaggtt cggcaccgag gtcgccgatc ctggacatca taaacggact
     4021 gcggctggcc ccgtgtcccg actgaccggt tttcatcctg gagacgccat gagccacaac
     4081 ttgttcaaca ccctgcagga gttctcccct gctccgggca aaaaggcgaa gttctattcg
     4141 ctgcctgcgc tggagaaggc cggtatcggc aagatctccc gcctgccggt ttccctccgt
     4201 atcgtgctgg aggcagtgct gcgcaacgtg gacggcaaaa aagtggcaga ggagcacgtc
     4261 aggcagctgg ctggctggcg accaaacgcg ccgcgcactg aggagatccc gttcgtggtc
     4321 gcacgcatcg tgctccagga cttcaccggc gtgccgcttt tggcggatct ggccgcgatg
     4381 cgcagtgtag ccgccaggat gggcggaaat ccgaagttga tcgagccgct ggtgccggtg
     4441 gatctggtgg tggaccactc ggtgcaagtg gaccactacg gcagcaagga agcgctggac
     4501 ctcaacatga aactggaatt ccagaggaac aaggagcgct accagttcat gaaatggggt
     4561 atgcaggcgt tcgacacgtt caaagtcgtg cccccgggtg tgggcatcgt gcaccaggtg
     4621 aatctcgagt acctggcccg cggcgtgcac gcgagagacg gcatttatta tcccgacacc
     4681 ctggtgggga ccgacagcca caccaccatg atcaacggca tcggcgtggt cggttggggc
     4741 gtcggcggca tcgaggccga agcaggcatg ctcggccagc cggtttattt cctcacgccc
     4801 gacgtggtcg gcgtgaacct caggggcgcg ctgcgcggcg gcgtcaccgc caccgatctg
     4861 gttctgacca tcaccgaaat gctgcgccgg gccaaggtgg tgggcaagtt tgtcgagttc
     4921 ttcggcgagg gcgtggagaa cctgtcgctg cccgatcgcg ccaccgtcgc caacatggct
     4981 cccgagtacg gcgccaccat gggtttcttc ccggtggacg acgccaccat cgagtacttc
     5041 aagggcaccg gccgcactcc cgaggaaatc gaagcgttca agtcctactt caaggcgcaa
     5101 ggcctgtacg gcatccccag gaagggccag atcgactaca gccaggcact ggagctgaac
     5161 ctgtccggcg ttgcgccatc gctggccgga cccaagcgcc cgcaggatcg catagagatc
     5221 ggcaaggtga agaacgcctt ttcgactctt ttctacaagc cggcggccga gaacggctac
     5281 aacaggccgg ccggcgcgct cgagcgccgc tatgacagct ggttccgcgg ctccggcacg
     5341 atgagcggcg gcggcaccag caattccgcc accattgccg agggtgcccc ggctcatgtg
     5401 gtggagatgg tgaacaaccg ccccaccccg gacgtggtca acgatgcgca tttaggcgcg
     5461 ccggttcgca tcacgctcgg ccacggcgac gtgctcatcg ccgccatcac ctcctgcacc
     5521 aacacttcaa accccggcgt gctgctcgcg gccggtctgt tagcgaagaa ggcggtcgag
     5581 cgcggactga gcgtgaagaa gcatgtcaaa acttccctcg cccccggatc gcgcgtggtc
     5641 accgagtacc tgaccaagtc gggtttgctg ccgtatctcg agaagctcgg cttctacctt
     5701 gcgggctacg gttgcaccac ctgcatcggc aactcggggc cgctcgatcc gggcatcgag
     5761 gaaactatcg tcaagaacga tctggtgggc agcgcggtgc tttcgggaaa ccgcaatttc
     5821 gaagcgcgca tccacgccaa catcaaggcc aattttcttg cttcgccgcc gctggtggtg
     5881 gcctatgcca ttgccggcac tgtgctcaag gacctgatga gcgaaccgct gggcaaggat
     5941 gcgaaaggcg aggacgtatg gataggcgac atctggccca gcagccagga aatttacgac
     6001 tgcatgaagt acgcgatgga tgccaagact ttccgccggc tgtactcgaa ccccggaaag
     6061 gacaatccgc tgtggcaggg tgtgccctcg gcctcgggtc aggtgtacaa ctggcccaaa
     6121 tccacgtata tcgcggagcc gcctttcttc cgcgatttca gcatgcagcc gggacggacc
     6181 ggcaacgttt tcggcgcccg ggtgctgggc atcttcggcg actcggtcac gacggaccac
     6241 atcagcccgg ccggttcgat ttccgaaaac tcgccggccg gaaagtacct cctcgccaac
     6301 ggggtcgcaa aggccgactt caattcctat ggtgcgcgcc gcggcaacca cgaagtcatg
     6361 atgcgcggca cgtttgccaa cgtgcgcatc aagaacctga tgctgcctcc caggccggat
     6421 ggttctcggg tcgaaggcgg ggtaaccctg ttccagcctt cgggggaaca gatgtccatt
     6481 tacgacgcgg cgatgaagta catagcccag ggaacgccca cggtggtgtt tgccggcgag
     6541 gagtacggca ccggttcctc gcgcgactgg gcggccaagg gcacgcagtt gctgggcgtg
     6601 aaggccgtca tcgccaagag cttcgagcgc atccaccgct ccaatctggt cggcatgggt
     6661 gtgctgccgc tgcaattcaa gggctccgat tcggtcgagc tgctgggaat caagggcgac
     6721 gacgagttcg acattctcgg cgtagagggt gaactcaggc cgcagcagga cctcaagctg
     6781 gtggtcaagc gcaccgacgg ctcgcgtgcc gaagtgaaag ttctgctgcg catcgacact
     6841 ccgatcgagg tggactacta cagacacggc ggaatcctcc cctatgtgtt gcgcgggctt
     6901 gtcgctgcgg cctagcagcg acttcgctcc ctgtccccct ctctacactg cgcgtggaga
     6961 gggccggggt gaggctgtgc tgcgcgagct tgtcgccgct tgaaccgagg acggctgccg
     7021 cccggccgtc acactgatca accctgccgt gcgctttaac tgcgactcgc atgccaggcg
     7081 cacaggccgc gtgaaacctg gcgaaccgcc actcgggcgg tggcgccatc tgcgcgacga
     7141 cgaatctttc tagggacagc cagacccgta ctgccaagcg tcaaccgcaa gggacgccaa
     7201 gtacgccagg gaaatacaaa tgaaaagaga agagaagtgt taaacgcttg cgcaagtatc
     7261 ttggcggaga ccagcccggg ccgcttgcgg tccccgtcac cttcctggcc ttcctttccc
     7321 tggcgtcctt ggcggtgaat acttagattc cccatcctcc ccatgcgcat tgttctcgac
     7381 accaacgtct ggctggactg gctggtgttc aacgatcccg cggtgatacc gctgaagtcg
     7441 gcgcgcagcg acgggctgat tcgcatcgca ataaattcag ccggcctgga agagctcggc
     7501 cgcgtgctcg catatgccga gttcaagctg gatgcggccg cccgcagtgc tctgttcact
     7561 caagttcgca actgcacgta ccgggtggac accgtgctga tctcacccct gccgcgctgc
     7621 gccgatccca atgatcagaa atttctggag ctcgcccgcg acgcacaagc cgactggctc
     7681 atcaccaaag acaaggcatt gctgcgtctc gccaagaaac aattctctgc tgtggcgttc
     7741 aggattggca cgccggagca atggttggca agtcgttgaa aaattggttg cgttcgaaag
     7801 cgtcaaaccg cttctctatt cgatccatgt cgccacttcg gcaacgggcg cccgctcggt
     7861 gcggtaccgg tggatcggat gcttggcatc ggcgtacccg aaggagatgc cgcagacgag
     7921 cttgcggttc gccggcagac cgaagcggct gcggatcagg tctgggtacg aggccagggc
     7981 ggcctgggca atcga
//
LOCUS       DASQOP010000069        11245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_27018, whole genome shotgun sequence.
ACCESSION   DASQOP010000069 DASQOP010000000
VERSION     DASQOP010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11245
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27018"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..684
                     /locus_tag="VE030_02570"
     CDS             82..684
                     /locus_tag="VE030_02570"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZF22332.1"
                     /translation="MSLKHSYTLIAPVYDAIIRRPLGGARRRSLAALPADKPCRILVD
                     GIGTGLDLPHLPALHEYLGIDLTAAMLRRAQARIGSLDALLVQGDSQRLPFRNECFDH
                     AILHLIVAVVPDGEAVLKETARVLKPAGRIFVLDKFLRRGERAPLRRLLNLLTRHVAT
                     RTDVVFEQLVEKVPQLRVVKDEPALLGGWFRLIEIQKTLR"
     gene            860..2095
                     /locus_tag="VE030_02575"
     CDS             860..2095
                     /locus_tag="VE030_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HZF22333.1"
                     /translation="MHGRKSLAVALCVLVLSAGGCASLTKTPSPAPPVTVEVLPETPA
                     PVSPAPEPAPAPQPPPHPQTFQATDWSALPDWGADRLAEALPALLLSCGSLKSQPEWQ
                     AACAAAAQIHGEDPAAVRQFFETSFVPYAVHNADGSAEGLVTGYYEPLLRGSRTPGKI
                     FRFPVYGVPDDLLIVDLTEAQPNLKGMRLRGRLQGNKVVPYYTRAEIERGDAAMRGRE
                     ILWVDDAVELFFLQVQGSGRVKLPSGEIVRIGFAEHNGHPYKSIGRVLVERGELPQDK
                     ASMQGIKQWGKQNPDLLPLLLQQNPAYVFFREMPSSSPGPVGALGVPLTPGRSIAVDS
                     SVVPLGTPVFLSSTWPLSARPLNRLVIAQDTGSAIKGAVRADFFWGFGDEAGQIAGRM
                     RQNGRMWVLYPRGFEPRLP"
     gene            complement(2302..2982)
                     /locus_tag="VE030_02580"
     CDS             complement(2302..2982)
                     /locus_tag="VE030_02580"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HZF22334.1"
                     /translation="MRRIRQLPQGASQTAFERLSVAVVLSATLHLYLIYGLALQPAHS
                     PPDRLSIINARLLPEPAVTKRPAAPAVQRGRIVPVQPRVSSLPEPVETPVPAAQAPPA
                     GDRSPEPEDSMASFPDPVHYAARDLDVYPQPLNRIEPVYPQTALAGEIGGSVTLLLLI
                     DESGRVTNASVVDAFPPDVFDESALRALAATAYSPAQKNGRVVRSRILVKVDYDPAAT
                     EGKDPIKR"
     gene            complement(3051..3791)
                     /locus_tag="VE030_02585"
     CDS             complement(3051..3791)
                     /locus_tag="VE030_02585"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HZF22335.1"
                     /translation="MTIRALTLFSSIILLFSGVACADEASIRSEIIKKFPKAHVESVT
                     KTSHLGLYEVVVDGQLFYTDEDFNYLIDGSIIETKSMTNVTAARQRDLEELKLRKLAF
                     PFEQLPFELAIKKVKGDGSRKFAVFSDPDCPYCKRLERDLVKVDNVTIYVFLYPLVEL
                     HPKAPDVARAIWCSGERVKAWDEYMLKGVVPKAAGTCANPVDKLVEFGKSKQITGTPT
                     IFFADGKRVPGAIPVDQIEDNLAKAQTR"
     gene            complement(3848..5011)
                     /locus_tag="VE030_02590"
     CDS             complement(3848..5011)
                     /locus_tag="VE030_02590"
                     /inference="COORDINATES: protein motif:HMM:NF005788.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF family hydroxylase"
                     /protein_id="HZF22336.1"
                     /translation="MKVDIAIVGGGLVGGSIARALGQSGLSLAMVEPRSPRPLPGTGF
                     DLRVYALNPGSVRCLEGWGVWPRLAQERIAPVQEMLVFGDDGSSKLEFSAYRNGVFEL
                     AAIVEESNLQQALTGCLDGQTDFVLLSGVECLRADWRESAAVLALSDGTELEARLVIA
                     ADGAESRMRSQAGIEVEVRDYGQRGVVASFDVERPHRNVAFQWFRKDGVLALLPLPQG
                     QVSMVWSTADAHAQDLLAMGGEELAGAVGDAAAHRLGAMRINGAAAAFALRRLRATRL
                     IAPRLALLGDAAHNVHPLAGQGLNLGIADAESLAEVIGGRGLESDCGATALLRRYERS
                     RKEDILAMECVTDGLHKLFSSSLPGLSRLRNAGLNLVDQLSPLKKLLVKRALG"
     gene            5140..5216
                     /locus_tag="VE030_02595"
     tRNA            5140..5216
                     /locus_tag="VE030_02595"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5174..5176,aa:Met,seq:cat)
     gene            5446..5718
                     /locus_tag="VE030_02600"
     CDS             5446..5718
                     /locus_tag="VE030_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22337.1"
                     /translation="MSPAFPARNFPEKAIRGELKSHDFPYVKIGDQTLRLAAGGRIFN
                     QQNLIIMPASLQSQTAQIMYTTDMNGDLSRVWLLTAEEAAQYPIKK"
     gene            5725..7059
                     /gene="miaB"
                     /locus_tag="VE030_02605"
     CDS             5725..7059
                     /gene="miaB"
                     /locus_tag="VE030_02605"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HZF22338.1"
                     /translation="MGKLYIRTFGCQMNEYDSDKMADVLAAAEGLEATDDPEQADVIL
                     LNTCSVREKAQEKVFHDLGRIRPLKQARPELIIGVGGCVASQEGAAIVARAPYVDLVF
                     GPQTLHRLPQMIRARRSSGKPQVDISFPEIEKFDNLPPARVEGCTAFVSIMEGCSKYC
                     SFCVVPYTRGEEVSRPFDDVLTEVAGLADQGVKEITLLGQNVNAYRGSMGDGEVADFA
                     LLLEYLAEVPGVERLRYTTSHPKDFSERLTNAHARIPQLVSHVHLPVQSGSDRVLCAM
                     KRGYTALEYKSIIRRLRAARPDAAISSDFIVGFPGEADEDFEQTLRLVEDIGFASSFS
                     FMYSPRPGTPAASLPDQVPQATKQARLARLQARLDAQALAISSRMVGSIQRILVEGPA
                     KKNPEELAGRTSSNLMVNFRGPQRLIGRMTDILITTAMSHSLRGEMPVKEAV"
     gene            7056..8009
                     /locus_tag="VE030_02610"
     CDS             7056..8009
                     /locus_tag="VE030_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HZF22339.1"
                     /translation="MKPFELSFSPVDNERLANLCGALDENLKQIETALDVSIARRGEN
                     FSVSGSAEQTRLAAQALQRFYDQANRNLGLEEIQLGLIEVAGGPNPLAADEALVLHTR
                     RRDLHGRTPRQVQYIRQIKEYDITFGIGPAGTGKTYLAVACAVDAFERDSVKRIVLVR
                     PAVEAGERLGFLPGDMAQKVDPYLRPLYDALYDLMGFEKVGKLFERGSIEIAPLAYMR
                     GRTLNHSFIILDEAQNTTPEQMKMFLTRMGFGSKAVVTGDVTQIDLPKGHKSGLIEAQ
                     LILAEVRGIAFTQFLSEDVVRHPLVQRIVHAYEQRGSRREG"
     gene            8016..8501
                     /gene="ybeY"
                     /locus_tag="VE030_02615"
     CDS             8016..8501
                     /gene="ybeY"
                     /locus_tag="VE030_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HZF22340.1"
                     /translation="MARAGGRHARSKPPYGLALTVQYAAEIDGLPTRSQVRAWVKAAL
                     QRDADVTVRLVGAEEGRTLNRRYRGRHHATNVLTFAYPESDQLSGDIALCVPVVEREA
                     REQHKNREAHYAHLVVHGMLHLQGYDHEKPADARLMETLETEIVTGLGYADPYKNKDE
                     G"
     gene            8616..9452
                     /locus_tag="VE030_02620"
     CDS             8616..9452
                     /locus_tag="VE030_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HZF22341.1"
                     /translation="MDEEQDKPSLMERLGALLMREPEDREQLIELLQRSYERNLLDAD
                     ALAMIEGVLQVSEMQARDIMIPRSQMDVIDIGDAPEKFIPWVIETGHSRFPVIGDNKD
                     DVIGILLAKDLLRYYAGEEFNVREMLRPAVFIPESKRLNVLLKDFRSNRNHIAIVVDE
                     YGGVAGLVTIEDVLEQIVGEIEDEFDFDETEANIVLDRNGQFRVKAQTEIDDFNRTFG
                     TDFNDEDFDTVGGLVLAHLGRLPKRGEQFRIGDLNFKVVRADSRRLHSVLVEKVRTES
                     AA"
     gene            9442..11031
                     /gene="lnt"
                     /locus_tag="VE030_02625"
     CDS             9442..11031
                     /gene="lnt"
                     /locus_tag="VE030_02625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HZF22342.1"
                     /translation="MRPELSARRESGAAAAVLVRLFLLPFLLGAVTVAGFAPFHIYPL
                     PILALSGLLLLWERCRNIKSAAAIGFCFGLGMFLTGVSWVYVSLHDFGGMGMLTAAIF
                     TFLFCAIPACYPALIGAILKRLPASRPARLLLFFPALWALAEWVRGWLFTGFPWLAIG
                     YSQAPGSPLAGFAPILGVFGVSLATAVAAAALASVLARVAEWRLQPPQRRMGLLIREP
                     GAWIIIALLVAGIASSEREWTQPLPGEPTSVALLQGNIPQEMKWRPERARATLETYLR
                     LAQSAEARLILLPETALPMFNVDVPREYLEMLADQGRRNGGDLLVGVPELEASGRYYN
                     SVMSFGSAPAQIYRKHHLVPFGDYFPLRSVLGWIMNLLHIPMSDFSRGEPVQRPIQAA
                     GQKIAVNICYEDVFGEEIIRQLPEATVLANFTNDAWWGKSVASEQHLQISQMRAIETG
                     RPMLRATNTGVTAIIDPKGRIVASAPGFTVTTVTGKIHGYHGATPYVRLGNWAFLALA
                     ALMIALPLALQRYSQRGDRGP"
BASE COUNT         2150 a         3548 c         3429 g         2118 t
ORIGIN      
        1 ttttttgctc ctgctcttcc tccgtgtcct ccgtgtactc cgtggtgaga tattcgctgt
       61 tcttcgttgg catcgggggc catgtcgctc aagcatagtt acacgctgat cgccccggtt
      121 tacgacgcga tcatccgccg gccgctcggc ggagcccgcc gccgcagtct tgccgcgctg
      181 ccggccgata aaccctgccg gattctggtg gacggcatcg gcaccggcct ggacctgccg
      241 caccttcccg cgttgcacga atatctggga atcgacctga ctgccgcgat gctgcgccgg
      301 gcgcaggcgc ggatcggttc actggacgcc ttgctggtgc aaggagacag ccaaagactg
      361 ccgttcagga atgaatgctt cgatcacgcg atcctgcacc tgattgtcgc agtcgtgcct
      421 gacggcgagg ccgtgctgaa ggagacggcc agggtcctca aaccggcggg aaggattttt
      481 gtgctggaca agttcctgcg ccgcggggag cgcgcgcctc tgcgcaggct gctcaacctg
      541 ctgacccggc acgtcgccac ccgaaccgac gtcgtgttcg aacagctcgt cgaaaaagtg
      601 ccgcaactac gcgtcgtgaa ggacgaaccg gcgctgctcg gcggctggtt ccgtttgatc
      661 gagatccaaa agacgttgcg ctagttttcc ttgtcgcggc gcggcttgcg cggcagcgtc
      721 caccagatga tgaacacgaa cagcgccagc gccaggaggg cttcgaaaat cagccagatc
      781 atttccgtaa aaaccggaag gcccgttgca tcgctttgct atactcgcag ccaaaataag
      841 tcacgctgaa gatcaccgaa tgcatggaag aaaatcgctg gcggtcgcgt tgtgtgtgct
      901 cgtgctgtct gccggcggtt gcgcaagtct aaccaagacc ccgtcgcccg cgccaccggt
      961 aacggttgag gtgcttcccg aaacaccggc gccggtcagc cccgcgcctg aacccgcgcc
     1021 cgccccgcaa cctccaccac acccgcagac cttccaggcc accgattgga gcgcgttgcc
     1081 cgactgggga gccgaccgac ttgccgaggc tttgccggcg ctgctgctct cgtgcggcag
     1141 cctgaaatcc caacccgagt ggcaagcggc ttgtgccgcc gcggcgcaga tccacggcga
     1201 agatccggcg gcagtgcggc agttcttcga aaccagtttc gttccctatg ccgtccacaa
     1261 cgccgacggc agcgcggagg gcctggtcac cggctactat gagccgctgc tgcgcggcag
     1321 ccgtacgcca ggcaagatat ttcgctttcc ggtgtacggg gttccggacg atcttctgat
     1381 cgtcgacctc accgaagccc aaccgaatct caaaggcatg cgactgcgcg gcaggctgca
     1441 gggcaacaag gtggtgccct attacacgcg cgcggaaatc gagcgcggcg atgccgcaat
     1501 gcgcggccgc gaaattctct gggtggacga cgcggtcgaa ctcttcttcc tgcaagtcca
     1561 ggggtcgggc cgggtcaaat tgcccagtgg agaaatcgtg cgcatcggct tcgccgagca
     1621 caacgggcac ccctacaaat ccatcgggcg cgtgctggtc gaacgcggcg aattaccgca
     1681 ggacaaggcg tcaatgcagg gaatcaagca atggggcaag cagaacccgg acttgctgcc
     1741 cctattgctg cagcagaatc cggcatacgt gttcttccgc gaaatgccgt cttcgtcgcc
     1801 ggggccggtt ggcgcgctcg gcgtccccct caccccggga cgcagcatcg cggtcgattc
     1861 ttccgtcgtg ccgctcggta ccccggtttt cctttccagc acttggccgc tttccgcgcg
     1921 gccgctaaac cgcctggtca tcgcgcagga taccggcagc gcgataaagg gtgcggtgcg
     1981 ggcggatttc ttctggggtt tcggcgatga agccgggcag atcgccggac gcatgcgcca
     2041 gaacggccgg atgtgggtgc tctatcctcg cggcttcgag cctcgcctgc cctgacagaa
     2101 aaaccctcac cacggaggac gcggaggaca cggaggaaaa ccaaaaacga ggaaaagcaa
     2161 atgggggtta agagcaaagt gactgttagt gacccgccac cacttttcga ctttgctgga
     2221 gcatctgtca attagagctt tttacactcg tttgcttttt ttgctcctgc ctttcctccg
     2281 tgtcctccgc gtcctccgtg gttagcgctt aattggatct tttccttcag ttgcggccgg
     2341 gtcgtagtcg actttgacga gtatgcggct gcgcaccacg cggccgttct tctgcgcagg
     2401 agagtatgcg gtcgccgcca gggcccgcaa cgccgactcg tcgaatacgt ccgggggaaa
     2461 cgcgtccacc accgatgcgt tggtgacccg cccggattca tcgatcaata gcagcaacgt
     2521 cactgaaccg ccgatttcgc cggcaagtgc cgtttgcgga tagacaggct cgatgcgatt
     2581 cagcggttgc ggatagacgt cgagatccct tgcggcgtaa tgaaccggat cgggaaagct
     2641 ggccattgaa tcttccggct ccgggctgcg atccccggcc ggcggagctt gcgcggccgg
     2701 taccggtgtc tcgaccggtt caggcaacga tgaaacccgc ggctgcacag gtacgatgcg
     2761 cccgcgctgc accgcaggcg ccgcgggccg cttcgtcacg gcgggttcgg gcaacagccg
     2821 ggcgttgatg atgctcaggc gatccggggg gctgtgggcc ggctgcagcg ccagcccgta
     2881 gataaggtac aggtgcagcg tcgcggacaa caccactgca acagacaggc gctcgaacgc
     2941 cgtctgggac gcgccttggg gaagctgcct gatcctgcgc accgcccgat cccgtactgg
     3001 aaacgccgac gccccgattc tagagcgccg gcggcgatgc cgggaaagat ttacctggtc
     3061 tgcgccttcg cgaggttatc ctcgatctgg tcgaccggaa tcgccccggg aacacgcttt
     3121 ccatccgcaa agaaaatcgt gggcgtaccg gtaatctgtt tggatttgcc gaattccacc
     3181 agcttgtcca cggggttggc gcaggtgcct gccgctttcg gtactacacc cttgagcatg
     3241 tattcgtccc aggccttgac gcgctcgccc gaacaccaga ttgcacgcgc aacatccggg
     3301 gctttcggat gcaactcgac caatggataa aggaagacat agatggtcac gttgtccacc
     3361 ttgacaaggt cgcgctcgag ccgcttgcag tacggacaat ccggatcgga aaacacggcg
     3421 aacttgcggc tgccgtcgcc cttgactttc ttgatcgcca gttcaaaggg aagttgctca
     3481 aaagggaaag ccagtttcct gagcttcagc tcctccagat ccctctggcg cgcggccgtc
     3541 acgttggtca tgctcttggt ttcgatgatg ctgccgtcga tcaggtaatt gaagtcttca
     3601 tcggtataga acagctggcc gtcgacaacc acttcatata atccgagatg ggatgtcttg
     3661 gtcacgcttt cgacgtgggc cttgggaaac ttcttgatga tctcggagcg aatggaggcc
     3721 tcatccgcgc aggcgacgcc ggagaacagc aggatgatcg atgaaaatag ggtcagggcg
     3781 cgaatggtca tgacggatcg gccgggaaaa gtccggattt ttcgttgaga gggcattttt
     3841 agaccgctta gccgagcgct cgtttcacta ataacttctt gagcggcgag agttgatcga
     3901 caagattcag ccccgcattg cgcaggcgcg acaggcccgg cagggagctg ctgaaaagct
     3961 tgtgcagccc atcagttacg cactccatcg ccagaatgtc ttccttgcgg ctgcgttcgt
     4021 atcgccgcaa cagcgccgtt gccccgcaat cgctctccag gccgcggccg ccgatgacct
     4081 cggcaagcga ttctgcatcg gcgattccga ggttcaatcc ctgcccggca agcgggtgca
     4141 cattgtgcgc ggcgtcgccc agcagggcaa ggcgcggcgc gatcaaacgg gtggcgcgca
     4201 ggcgtcgcaa cgcaaatgcg gcggcggctc cgttgatccg cattgcgcca agcctgtgtg
     4261 cggccgcgtc cccgaccgcg cccgccagct cttcaccgcc cattgccagc agatcctgtg
     4321 catgcgcgtc cgcggtggac cacaccatgg acacctggcc ctgcggcaga ggcagcagcg
     4381 cgagcacgcc atccttgcgg aaccactgga acgccacgtt gcgatgcggc ctctcgacat
     4441 caaagctggc gaccacgccc ctctgtccgt agtcgcgaac ctcgacctcg attcctgcct
     4501 gcgaacgcat tctcgactcg gcaccgtcgg ccgcaatcac cagccgcgct tccagctccg
     4561 tgccgtcgga gagcgcaagc acggctgcgg actccctcca gtccgcgcgc aggcattcga
     4621 cgccgctcaa gagcacgaaa tcggtctgcc cgtcgagaca tccggtcagc gcctgctgaa
     4681 gattggattc ctccacgatc gccgcaagct cgaagacgcc gttgcgatag gcactgaact
     4741 cgagcttcga gctgccgtca tcgccgaata cgagcatttc ctgaaccggc gcaatacgct
     4801 cctgcgcgag ccgcggccat acaccccaac cctccagaca gcgcacgctg cctggattga
     4861 gtgcatagac gcgcaagtcg aatcccgtac caggtaacgg acgtggcgat ctcggctcga
     4921 ccatcgcgag gctcaacccg ctttggccaa gcgcgcgcgc gatactgccc ccgaccagac
     4981 ctccgcctac aatggcgatg tcgactttca tgcagccagt ctactacggc cgtgcaggcg
     5041 cgcgcccagc ccggttcctg ccttgacaat gcagggggcc gtcagtaccc tttcgctccc
     5101 ttttcctccc cgattcagaa agaggattgg caggtttccg gtgatatagc tcagctggtt
     5161 agagcacagc actcataatg ctggggtcgg tggttcaagt ccacctatca ccaccaccga
     5221 acgtggcagt gcgcggccgc ggacgtgccc aaacgcggcc gcaatctgcc ccccgcgact
     5281 caaccacgag atgcgacggc gctgtcaaca tgagcgcaac gcggtatact tttaagtctc
     5341 tgtttccttt gtggtagtca cttgagaatt gcagaggtcc ggtcatccat gcgtgtgcgc
     5401 agaatcgccc tgcggtttct ggtcgcgatg attggcgtaa ctttaatgtc gcctgcattc
     5461 ccggcccgca atttccccga gaaagcaatt cgcggcgaat tgaagtcgca cgattttccg
     5521 tacgtgaaga tcggcgacca gaccctccgc ctcgccgccg gcggcaggat tttcaaccag
     5581 cagaacctca tcatcatgcc ggcctcgctg cagtcgcaga ccgcgcagat catgtacacc
     5641 acggatatga acggcgacct gtcgcgcgtg tggctgctga ccgccgaaga agcggcgcag
     5701 tatccaatca agaaatagcg cggcatgggc aagctttaca tccgcacctt cggctgccag
     5761 atgaacgagt acgactcgga caagatggcc gacgtgctcg ccgccgcgga aggtttagaa
     5821 gctaccgacg accccgaaca ggccgacgtc atcctgttaa acacctgctc ggtgcgcgag
     5881 aaggcccagg aaaaagtatt ccacgacctc gggcgtatcc ggcccctgaa gcaggcccgg
     5941 cccgagctca tcatcggcgt tggcggctgt gtggccagcc aggagggcgc ggcgatcgtt
     6001 gcacgcgcac cctacgtcga cctggtcttc ggcccgcaga ccctgcaccg gttgccgcag
     6061 atgattcggg cgcggcgcag cagcggcaag ccgcaggtgg acatctcctt tcctgaaatc
     6121 gaaaaattcg acaacctgcc gccggccaga gtcgagggat gcacggcctt cgtctcgatc
     6181 atggagggct gcagcaagta ttgcagtttc tgcgtggtgc cctacacgcg cggcgaagaa
     6241 gtctcgcgcc cgttcgacga tgtgcttacc gaagtggccg ggctggcgga tcagggcgtg
     6301 aaggagatca cgctgctcgg acagaacgtc aatgcctatc gtggcagcat gggcgacggt
     6361 gaagttgccg actttgccct gttgctcgag tacctcgccg aagttccggg cgtcgagcgc
     6421 ctgagatata ccacgtcgca tccgaaggac ttcagcgagc gcctgaccaa tgcccacgcc
     6481 aggatcccgc agctggtgag tcacgtgcac ctgccggtgc agtccggctc ggaccgggta
     6541 ctttgcgcaa tgaagcgcgg ttacaccgcg ctggaataca aatccatcat ccgccgcctg
     6601 cgcgcggcac ggccggacgc agccatctct tccgacttca tcgtcggctt tcccggcgag
     6661 gccgacgaag acttcgagca gactctgcgg ctggtcgagg acatcggttt cgcttcgagc
     6721 ttcagcttca tgtacagccc gcgccccggg acgcccgcag caagtctccc cgaccaggtg
     6781 ccgcaggcga ccaagcaggc acgcctcgca cgcctgcagg cgcggctcga tgcgcaggcg
     6841 ctggcgatca gcagccgcat ggtcggttcg atccagcgca tcctggtcga ggggcccgca
     6901 aagaagaatc cggaagaact ggcgggccgt accagcagca atctcatggt caattttcgc
     6961 ggcccgcagc gcctcatcgg gcggatgacc gacatactca tcacgacggc gatgtcgcat
     7021 tcgctgcgcg gcgaaatgcc ggtaaaagaa gctgtttgaa gcccttcgaa ctttctttct
     7081 cccccgtcga caacgagcgc ctcgccaacc tgtgcggcgc gctcgacgaa aatctcaagc
     7141 agattgaaac cgcgctcgac gtgtccattg cgcgccgcgg agagaacttc agcgtctccg
     7201 gcagcgccga gcagacccgg ctggccgccc aggcgctgca gcgtttttat gaccaggcga
     7261 accgcaatct gggcctcgaa gaaatccagc tcggcctgat cgaggtcgcg ggcggaccga
     7321 atccgttggc agcagacgaa gccctggtgc tgcacacgcg gcgccgggac ttgcacggcc
     7381 ggaccccgcg ccaggtgcag tacatccggc agatcaagga gtacgacatc actttcggca
     7441 tcggcccggc cggtaccggc aaaacttatc tagcggtcgc ttgcgcggtg gatgcattcg
     7501 agcgcgattc cgtgaagcgg atcgtgctgg tgcgccccgc agtcgaagcc ggcgagcgtc
     7561 tcggtttcct gcccggagac atggcgcaga aggtcgatcc ctacctgcgg ccactctacg
     7621 acgcgctcta cgacctgatg ggcttcgaga aagtcggcaa attgttcgaa cgcggctcga
     7681 tcgagatcgc gccgctcgcc tacatgcgcg gccgcacgct caatcactcc ttcatcattc
     7741 tcgacgaggc gcagaacacc acccccgagc agatgaaaat gttccttacc cgcatgggct
     7801 tcggcagcaa ggcggtggtc accggcgacg tcacccagat cgacctgccc aaggggcaca
     7861 agagcggtct gatcgaggca caactgatac tcgcagaagt gcgcggcatc gccttcaccc
     7921 agttcctcag cgaagacgtg gtgcgccacc cgctggtgca gcgcattgtc cacgcctatg
     7981 aacagcgcgg ctcccgcagg gaaggctgag cggcgatggc ccgcgccggc ggccggcatg
     8041 cgcgctccaa accaccgtac ggacttgcgt tgacggtgca gtacgcagcg gagatcgacg
     8101 gattgccgac gcgcagccag gtgcgtgcct gggtgaaggc cgcgttgcaa cgcgatgccg
     8161 acgttacggt gcgcctggtg ggtgccgaag aaggccgcac gctcaaccgc cggtatcgcg
     8221 gcaggcatca cgccaccaac gtactgactt ttgcctatcc cgaatccgat cagttgtcgg
     8281 gcgacatcgc actgtgcgtt ccggtggtcg aacgcgaagc ccgggaacag cacaagaatc
     8341 gggaagcgca ctacgcccat ctggtggtgc acggcatgct gcacctgcag ggttacgatc
     8401 acgagaagcc cgcagacgca cggctcatgg aaacgctcga aacggaaatt gtcaccggcc
     8461 tggggtatgc tgacccctac aagaacaagg atgagggatg aaggatgaag gatgaagtga
     8521 aaaacgattc gcgggtgcga gatgaggcgg agtccatggt ttgttttttg cctttccttc
     8581 cgccttcatc cttccgcctt catccttgat ttcatatgga cgaagaacag gacaaaccct
     8641 cattgatgga acggctgggc gcgttgctga tgcgcgagcc ggaagaccgc gagcagctga
     8701 tcgagctgct gcagcgttcc tacgagcgca acctgctgga tgccgacgca ctcgccatga
     8761 tcgaaggcgt cttgcaggtt tccgagatgc aggcgcgcga catcatgatc ccgcgttcgc
     8821 agatggacgt gatcgacatc ggcgacgcgc ccgagaagtt cattccctgg gtgatcgaga
     8881 ccggtcactc gcgtttcccg gtgatcggcg acaacaagga cgacgtgatc ggcatcctgc
     8941 tggccaagga cctgctgcgc tactacgcgg gcgaggagtt caacgtgcgc gaaatgctgc
     9001 ggccggcggt attcattccc gagtcgaagc gcctgaacgt cctgctcaag gattttcgca
     9061 gcaatcgcaa ccacatcgcg atcgtggtgg acgagtacgg tggcgttgcc ggtttggtga
     9121 ccatcgaaga cgtgctggaa cagattgtcg gcgaaatcga ggatgaattc gatttcgacg
     9181 agaccgaagc caacatcgtg ctggacagaa acggccagtt ccgcgtgaag gcgcagaccg
     9241 agatcgacga cttcaacagg acattcggca ccgacttcaa cgacgaggac ttcgacaccg
     9301 tgggcggcct ggtactggct cacctgggcc ggctgccaaa gcgcggcgag cagttccgca
     9361 tcggcgacct gaacttcaag gtggtgcgcg ccgacagccg gaggctgcat tcggtcctgg
     9421 tagaaaaggt caggacggag agtgcggcct gagttgtccg cccgccgcga gtcaggcgcc
     9481 gcggccgctg ttttagttcg actcttcctg ctcccgttcc tcctgggggc ggtcacggtc
     9541 gcgggattcg cgccgtttca tatttacccg ctgccgatcc tggcgctgag cggattgctg
     9601 ctgctctggg aacgctgccg caacatcaag tctgccgcag ccattggatt ttgcttcggt
     9661 ctcggcatgt ttctcaccgg cgtgagctgg gtatacgtca gcctgcacga tttcggcggg
     9721 atgggaatgc tgaccgccgc gatcttcacc tttttattct gcgcaatccc ggcctgttat
     9781 ccggcgttga tcggggcaat tctcaagcgt ctgccggcct cccggccggc acgcctgctg
     9841 ctctttttcc cggcgttgtg ggcattggcg gagtgggtgc gcggctggct gttcaccggc
     9901 ttcccgtggc tcgctatcgg ttactcgcag gcacccggca gcccattggc cggcttcgcg
     9961 ccgatactcg gggtgttcgg cgtgtcactg gctacggctg tcgccgcggc tgcgctggcg
    10021 tcggtcctcg cgcgcgtcgc cgaatggcgc ttgcagcccc cgcaacggcg gatggggctg
    10081 ctgattcgtg agcccggcgc atggatcatc atcgccctgc tggtcgccgg catcgcatcg
    10141 agcgagcggg aatggacaca acctttgccc ggcgagccga ccagcgttgc acttctgcaa
    10201 gggaatattc cgcaagagat gaagtggcgg ccggagcgcg cgcgcgcgac acttgaaact
    10261 tacctcaggt tggcgcagtc ggctgaagcc agattgatcc tgttgcccga gacggcgctg
    10321 ccgatgttca acgtggacgt cccaagggaa tatctggaaa tgctcgccga ccagggccgg
    10381 cgcaacggcg gcgacctgct ggtgggcgtg cccgagctcg aagcctcggg tcgttattac
    10441 aacagcgtga tgagcttcgg ctcggcgccg gcgcagatct accgcaagca ccatctggtg
    10501 cccttcggcg attattttcc gcttcggagc gtgctaggct ggatcatgaa cctgctgcat
    10561 atcccgatgt cggatttttc ccgcggcgag ccggtgcagc gccccattca agccgccgga
    10621 cagaagatcg ctgtcaacat ttgctacgaa gatgtgttcg gcgaagagat catccgccag
    10681 ttgccggagg caaccgtact ggccaatttc accaacgacg cctggtgggg aaaatcggtc
    10741 gcctcggagc agcatctgca aatctcccag atgcgcgcca tcgaaaccgg ccggcccatg
    10801 ttgcgcgcca ccaataccgg ggtgaccgcg atcatcgacc cgaaaggtcg catcgtggcg
    10861 tccgctcctg gtttcacggt cacgacggta accggcaaga ttcatggtta ccatggagca
    10921 acgccgtacg tgcgcctggg caactgggcg ttcctggcac tcgccgcact gatgatcgcg
    10981 ttgccgctgg cgttacagcg ttactcgcaa cgcggggata gagggcctta agatctctct
    11041 ctcaagaaaa cgctcatcgc ggagcaattg gataacgaaa ctctcaccgc ggaggacgcg
    11101 gaggaaaaac aagaacgagg aaaagcagga tggggataaa gagcggggtg actccatatg
    11161 atcctctccc gccaatttcg actttgctgg ggcatgcgaa actggagctt cctacactcg
    11221 tttgtatttt ctcctgtctt tcctc
//
LOCUS       DASQOP010000070         3763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1034702, whole genome shotgun sequence.
ACCESSION   DASQOP010000070 DASQOP010000000
VERSION     DASQOP010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034702"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /gene="hemW"
                     /locus_tag="VE030_02630"
     CDS             <1..963
                     /gene="hemW"
                     /locus_tag="VE030_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006496579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HZF22343.1"
                     /translation="GGGTPSLLSARALDELLSAIRARLPLAPDAEITLEANPGTFEAQ
                     KFADFRAAGVNRLSIGVQSFDPAHLKALGRIHDGAQARAAIEIAQASFDNFNLDLMYA
                     LPGQTVEQVLADIEAAIGFGPRHVSAYHLTIEPNTYFYRHPPQVPDQDVAADMQEAIE
                     ARLASAGYRHYETSAFARPGREARHNLNYWTFGDYLGIGAGAHSKLSFADRILRQMRV
                     KHPRDYLEKMAAGVPVQQEQRLAAEELPFEFMMNALRLIDGFELRLFTERTGVPVHTL
                     RKPLETAERSGLIERDHVRVRPTLRGQRFLNDLLQIFLGDSGTR"
     gene            complement(1044..1778)
                     /gene="trmB"
                     /locus_tag="VE030_02635"
     CDS             complement(1044..1778)
                     /gene="trmB"
                     /locus_tag="VE030_02635"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012213966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HZF22344.1"
                     /translation="MSPPWRTGTASSVSAPNTRPHIRSFVLRQGRVSNAQRRAVETLL
                     PTFGIAYAPALLDLDAAFGRPAPKILEIGFGMGETTAQIALAHPHNDYLGIEVHTPGV
                     GSLLRLIEAHALANVRIIQHDAVEVLNHMIAPGSLSATHVFFPDPWPKKRHHKRRLIQ
                     PSLVHTLASRLRPGGYLHLATDWEDYALQMLEVLSAEARLDNTAQGFAARPAYRPLTK
                     FETRGLKLGHRVWDIVFRRRAGDFEG"
     gene            complement(1780..2583)
                     /locus_tag="VE030_02640"
     CDS             complement(1780..2583)
                     /locus_tag="VE030_02640"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008159822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HZF22345.1"
                     /translation="MDQLVIAGKPYSSRLLVGTGKYQDFAQTRAAVEASGAQIVTVAI
                     RRTNIGQNPNEPNLLDALPPSYYTILPNTAGCYSAQDAVRTLRLARELLDGHDLVKLE
                     VLGDPHTLYPNMVETLAAAETLVKEGFKVMVYTSDDPILAKRLEEIGCVAVMPLASLI
                     GSGMGILNPWNLQIILSNAKVPVLVDAGVGTASDAAIAMELGCDGVLMNTAIAAARDP
                     VLMASAMKKAVESGREAFLAGRMPKKLYSAAPSSPTTGIIGTASKDRAA"
     gene            complement(2701..2901)
                     /gene="thiS"
                     /locus_tag="VE030_02645"
     CDS             complement(2701..2901)
                     /gene="thiS"
                     /locus_tag="VE030_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HZF22346.1"
                     /translation="MAITINGQSRSFPGPLNVARLIEKLDLKGKRIALERNGEIVPRS
                     KFDRVALADGDALEIVVAVGGG"
     gene            2947..3020
                     /locus_tag="VE030_02650"
     tRNA            2947..3020
                     /locus_tag="VE030_02650"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2979..2981,aa:Gly,seq:ccc)
     gene            3124..>3763
                     /locus_tag="VE030_02655"
     CDS             3124..>3763
                     /locus_tag="VE030_02655"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HZF22347.1"
                     /translation="MKLLKWILIAVGGVVVLFAGALAFIAATFDPNQYKGQIADLVKE
                     KTQRTLSIEGDIRLTLFPKLGVQLGKTRLSEFRSDQEFAGLDQMRVSLALLPLLSKQV
                     VVDQIQLNGLRVNLVKHRDGRTNLADLLGVEEVSTPAPAVTQPSAAAAPIEFDIDGVK
                     VRKAAVSWKDEAAGTEYTVSDFNLETGPVAPGVPVKFELAAAFTASVPKMEVK"
BASE COUNT          700 a         1129 c         1204 g          730 t
ORIGIN      
        1 ggcggcggca cgccctcgct gctgtccgca cgtgcgctgg atgaactgct gtcggcgatc
       61 cgggcaaggc tgccgctcgc gccggacgcc gagatcacgc tggaagccaa cccggggacc
      121 ttcgaggcgc agaaattcgc cgatttccgc gccgccggag tcaaccggct gtcgatcggc
      181 gtccagagtt tcgaccctgc gcatctcaag gcgctcggcc gcattcacga cggggcgcaa
      241 gctcgcgccg cgatcgagat cgcgcaagcg agtttcgaca acttcaatct ggatctgatg
      301 tacgcattgc cgggccagac cgtagaacag gtgcttgccg acatcgaagc ggccatcggc
      361 ttcgggcccc ggcatgtatc cgcgtatcac ttgaccatcg agccgaacac ctatttttat
      421 cgccatccgc cacaggtccc ggaccaggac gtggcggccg acatgcagga agcgatcgaa
      481 gcgcggctgg cctccgccgg ctaccggcac tacgaaactt ccgcgttcgc gcggcccggg
      541 cgcgaagccc ggcacaacct caactactgg accttcggtg actatctcgg catcggcgcc
      601 ggcgcgcata gcaaactctc gttcgccgac cggatcctgc ggcagatgcg cgtcaagcat
      661 ccgcgcgact acctggagaa aatggccgcc ggcgtgccgg tccagcagga gcagcggctt
      721 gccgctgaag agctgccgtt cgagttcatg atgaatgcgc tgcggctcat cgatggcttc
      781 gagctgcggt tgttcacgga acgcaccgga gtgccggtcc ataccttgcg caaaccactg
      841 gagactgcgg agcgatcagg cttgatcgag cgcgatcatg tgcgcgtgcg gccgacgcta
      901 cggggacagc gatttctgaa cgatctgctg cagatttttc tcggggattc cgggacgagg
      961 taactagatc aggggtggcc gtcatcagtt ggcattggct gcaaactgcc tgctgcaaac
     1021 tgccaacttt tttccttcat ccctcatccc tcaaagtcgc cggcacggcg acgaaagacg
     1081 atgtcccaca cccggtggcc gagcttcagc ccgcgcgtct cgaacttggt gagtgggcgg
     1141 taagcggggc gtgcggcaaa accctgggcg gtgttgtcga gccgcgcctc ggcggacaac
     1201 acctcgagca tctgcagcgc gtaatcctcc cagtcggttg cgaggtgtag atagccccct
     1261 gggcgcagcc ggctggccag cgtatgcacc aatgaaggct ggatgagacg gcgtttgtga
     1321 tggcgcttct tcggccaggg atcgggaaaa aagacgtgag tggcgctgag gctgccggga
     1381 gcgatcatgt ggttcagcac ttcgaccgcg tcgtgctgaa tgatgcgcac gttggcgagt
     1441 gcgtgggcct cgatcagccg caacaggctg ccgacgccgg gcgtgtgaac ttcgatgccg
     1501 agatagtcgt tgtgcgggtg cgccaaggcg atctgcgccg tcgtttcgcc catgccgaag
     1561 ccgatctcga ggattttcgg cgcaggccgt ccgaacgcgg catccaggtc cagcagcgcg
     1621 ggcgcgtatg caatgccgaa cgtcggcagc agcgtttcca cggcgcggcg ttgcgcgttg
     1681 gaaacgcggc cctggcgcag cacgaagctg cggatgtgcg gccgggtgtt gggggcactc
     1741 actgaagaag ccgtgccggt acgccacggc ggggacattt caggccgctc tgtccttgga
     1801 ggcggtgccg atgatcccgg tggtcggtga cgaaggggcg gccgagtaga gcttcttcgg
     1861 catgcgcccg gccagaaaag cctcgcgccc ggactcgacc gcctttttca tcgccgaggc
     1921 catcaggacc ggatcgcgcg ccgcggcgat tgctgtgttc atcaacacgc cgtcgcagcc
     1981 caactccatg gcgatcgccg cgtccgaagc cgtgcctacg ccggcgtcca ccagcacagg
     2041 aaccttggca ttggagagga tgatctgcag gttccatggg ttgaggatgc ccattccgga
     2101 accgatcagc gaggccagcg gcatcaccgc gacgcagccg atttcctcca ggcgcttggc
     2161 gaggatcgga tcatcggagg tgtataccat caccttgaaa ccttccttca ccagggtttc
     2221 ggcggcggcg agggtctcga ccatgttggg ataaagcgtg tgcggatcgc ccagaacctc
     2281 gagcttcacc aggtcgtggc catcgagcag ttcgcgcgcc aggcgcaggg tgcgcactgc
     2341 gtcctgcgcg ctgtagcagc cggcggtatt cggcaggatg gtgtagtacg acggcggcag
     2401 cgcgtcgagc agattgggtt cgttcgggtt ctggcctatg ttggtgcggc ggatcgccac
     2461 ggtgacgatc tgtgcgccgc tggcctcgac cgccgcgcgc gtctgcgcga agtcctggta
     2521 cttgccggtg ccgaccagca gacgggaact gtagggtttg cctgcgatga cgagttgatc
     2581 catgtctttt cgggtgattg agtgattggg taattgagtg attggccctg aatcactcaa
     2641 tcacttaatc cgccgttccc cattcccaat tgcccattgc ccatttccca ttccccgata
     2701 ttatcccccc ccgaccgcca ccacgatttc gagcgcgtcg ccgtcggcaa gcgccacccg
     2761 gtcgaacttg ctgcgcggca cgatttcgcc gttgcgttcc agcgcgatgc gcttgccctt
     2821 cagatccagc ttttcgatga ggcgggcaac gttcaagggg cctgggaacg accgcgattg
     2881 gccgttgatc gtaattgcaa tcacttcagg atacgagcca ttttcgttaa gttacaatgc
     2941 tagcatgcgg gtgtagttca atggtagaac gaaagcttcc caagctttag acgtcggttc
     3001 gatcccgtcc acccgctcca ctaaaaaatg gtccctcgtc ctccgtggta aatgattgtc
     3061 tgttacacgg actcgttgcc cagatcgcct catcagaaaa ccggatcaac cggagatcgc
     3121 ggaatgaaat tgctcaaatg gatactgatc gcagtcggtg gcgtggtcgt cctgttcgct
     3181 ggcgcgttgg cgttcattgc cgccactttc gatcccaatc agtacaaggg gcagattgcc
     3241 gatctggtga aggaaaagac ccagagaacg ctcagcatcg aaggcgatat caggctgacg
     3301 ctgttcccga agcttggcgt acaactgggg aaaacacgcc tgtccgagtt caggagcgac
     3361 caggagtttg ctggattgga tcagatgcgg gtgtcgctgg ctttgctccc gctgctgtcg
     3421 aaacaagtgg tcgtggacca gatccagctc aacggattgc gagttaatct ggtcaagcac
     3481 agagacggca ggaccaatct tgccgatctg cttggagtgg aggaagtctc gacacccgcg
     3541 cccgcggtga cgcagccgtc cgcggccgcg gccccgatcg agttcgatat cgatggcgta
     3601 aaggtgagga aggccgcggt gagctggaag gatgaggcgg ccggcaccga gtacacggtg
     3661 tccgatttca accttgagac cgggccggtg gctcccgggg taccagtcaa attcgagctt
     3721 gctgcggcat tcacggcatc ggtaccgaag atggaggtga agt
//
LOCUS       DASQOP010000071         2833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2845204, whole genome shotgun sequence.
ACCESSION   DASQOP010000071 DASQOP010000000
VERSION     DASQOP010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2845204"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..1108)
                     /locus_tag="VE030_02660"
     CDS             complement(53..1108)
                     /locus_tag="VE030_02660"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZF22348.1"
                     /translation="MNSIFRVRFAWYLAAAVAAGLLLYLVAPILSPFLFAAILAYICL
                     PLVDRLAKRMARWLAVSIVLVLLLVGLSLLVLIVAPMVEWQFAAFLQNVPGYLDWVRE
                     RFLPWLAGSTGVELHLDLEHAKQALADSLRADNDVLKSLLPKLTTGGAALIGFFTAVL
                     LVPVMLFYFLRDWHDFMRRIEDLIPRRSQQWAVSFTGEINSVLEEFLRGQIAVIVIMC
                     AYYVLGLWIAGLEYALPIGILSGLLVFVPYLGALVGLLLATLAGINQFDSIFELTGVW
                     VVFGIGQSLEGFVVTPWLVGDRIGLHPVAVIFALLVFGNLFGFFGVLLALPASAALLV
                     GLRHLRAQYLASDLYKS"
     gene            1264..2313
                     /gene="purM"
                     /locus_tag="VE030_02665"
     CDS             1264..2313
                     /gene="purM"
                     /locus_tag="VE030_02665"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HZF22349.1"
                     /translation="MKTDRPPSGSLSYRDAGVDIDAGDQLVENIKPFARRTLRDGVLA
                     GIGGFGALFEVPKGYREPVLVSGTDGVGTKLKLAFQLDRHDTIGIDLVAMSVNDILVQ
                     GAEPLFFLDYFACGKLAVDTATQVVKGIAAGCEQAGCALIGGETAEMPGMYPAGEYDL
                     AGFAVGVVEKSGIIDGAGIVAGDVVLGMASSGAHSNGYSLVRKIVETFGADLSSDFDG
                     RTLGEALLAPTRIYVRPVLAAMRAVRIKGLAHITGGGLVENVPRVLPSNVKAVLAKSA
                     WRRPKLFEWLQRNGNVAEAEMHSVFNCGIGMVAIVAAENAGRAQQAFEREGESVFRIG
                     EIRPRSGDEPQTVVL"
     gene            complement(2531..>2833)
                     /locus_tag="VE030_02670"
     CDS             complement(2531..>2833)
                     /locus_tag="VE030_02670"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HZF22350.1"
                     /translation="DVKQIREMRFPVFHGGIGPLDTKGRARMVQRDVPVECAGVRVRN
                     GDILFGDVDGVVVIPQERAAEVLAKAKEKVAGEGRTRDELRQGRLLHEVFEKYRIL"
BASE COUNT          606 a          884 c          868 g          475 t
ORIGIN      
        1 ttgtcgaacg ccggcaccgg ggatggggcc agtccgagag ccagctgcct catcacgatt
       61 tatagagatc gctggccaga tattgcgcac gcagatgccg caagcccacc aacagggccg
      121 cactcgcagg cagcgccagc agcacgccga agaatccgaa aagatttccg aacaccagca
      181 gcgcgaagat caccgccacc ggatgcagcc cgatgcggtc gcccaccagc cagggcgtga
      241 ccacgaaacc ttcgagggac tgtccgatgc cgaacacgac ccacacgccg gtcagttcga
      301 aaatactgtc gaactggttt atcccggcga gcgtcgcaag caacagaccc accagcgcgc
      361 cgagataggg gacgaatacc agcaagcccg acagtatgcc gatcggcagc gcgtactcca
      421 gaccggcgat ccagagcccc aacacataat aggcgcacat gatgacaatt acagcgatct
      481 gcccgcgcag gaactcctcg agcacgctat tgatctctcc ggtaaaactc accgcccact
      541 gttgcgagcg gcgcgggatc agatcctcga tgcggcgcat gaagtcgtgc cagtcgcgca
      601 gaaaataaaa cagcatcacc ggcaccagca gcacggcggt gaagaagcct ataagcgccg
      661 cgccgccggt ggtgagtttt ggcagcagcg acttgagaac atcgttgtcg gcacgcaaac
      721 tgtcggccag cgcctgcttc gcatgctcta ggtcgaggtg cagctccacg ccggtgctgc
      781 cggcaagcca tgggaggaag cgttcgcgca cccagtcgag atagcccggg acgttctgca
      841 gaaaggccgc aaactgccat tccaccatcg gcgccacgat cagaaccagc aacgatagac
      901 cgaccagcag cagcaccagc acgatcgaaa ccgcgagcca gcgcgccatt ctcttcgcta
      961 accgatccac cagcggcagg cagatgtagg ccaggatcgc ggcgaacagg aacgggctca
     1021 ggatcggtgc gaccagatac aggagaagcc cggcggcgac cgcggcggcc aggtaccacg
     1081 caaaacgcac acggaagatg ctgttcattt ggagtaaaat tcgcggcgca ccggccatgt
     1141 tcatgaatca tgcatggtta tgaatcatgc acgccaatga atcacgtacg gcaatgaatc
     1201 ccggcgtcgc attggccctg aatttcgacc gccactctat cctgaagaac ccggcagctc
     1261 aacttgaaga ccgaccgacc tccctctggc tcgctttcct atcgtgacgc cggcgtggac
     1321 atcgacgccg gcgaccagct ggtcgagaac atcaagccct tcgcaagacg cacgctcagg
     1381 gacggcgtgc tcgccggcat cggcggcttc ggcgcgctgt tcgaagtgcc gaagggatac
     1441 cgggaaccgg tgctggtatc tggtaccgac ggcgtcggga ccaagctcaa gctggcattt
     1501 cagcttgatc gccatgacac catcggcatc gacctggtcg cgatgagcgt caacgacatc
     1561 ctggtgcagg gtgccgagcc acttttcttc ctcgactact tcgcctgcgg caagcttgca
     1621 gtggataccg caacacaagt cgtgaaaggg atagccgccg gctgcgaaca ggctggctgt
     1681 gcgttgatcg gaggggagac ggcggagatg ccggggatgt acccggccgg cgaatacgac
     1741 ctcgccggct tcgcggtcgg cgtggtcgag aagtcgggga tcatcgatgg cgcaggtatc
     1801 gtggcgggcg acgtggttct cgggatggcc agcagcggcg cacactccaa cggttactcg
     1861 ctggtgcgaa agatcgtcga aactttcggc gcagacctgt catccgattt cgacggcagg
     1921 acgttgggag aagcgctgct ggcaccgacg cgcatatacg tgcggccggt gctcgcggcg
     1981 atgcgcgcgg tgcgcatcaa gggtcttgcg cacatcaccg gcggcggtct ggtggagaac
     2041 gtgccgcgcg tgctgccgtc taacgtaaag gcggtgctgg caaagagcgc ttggcggcgg
     2101 ccgaaactgt tcgaatggct gcaaagaaac ggcaatgtcg ccgaggcgga aatgcacagt
     2161 gtgttcaatt gcggcatcgg catggtggcg atcgtagccg cggagaatgc cggccgggcg
     2221 cagcaagcgt tcgagcgcga aggagaaagc gttttccgca tcggcgagat ccgcccccgc
     2281 tcgggcgacg aaccgcagac ggtggtgctc tagtgtcctg ttccgccaat tcgtggctga
     2341 aaacccgacg agaatcggcg ccatgcggtg ttacgagtcc tcgccgtggg gcccgccact
     2401 gtcgagcact cgcgccttgc ctgacgccga tttcgcgggt tttccgatcg atgttggatt
     2461 gctgttaaat aaattgtggg actgagttcc aagattcgtg agttatccca cgaattagcg
     2521 gaacaggaca ctagagaatc cggtacttct cgaacacctc gtgcagcaga cggccctgtc
     2581 gcagctcgtc gcgcgtccgg ccctctcccg ccactttctc ctttgccttc gcgagaactt
     2641 cggcggcgcg ttcctgcgga atgaccacga cgccatccac gtccccgaac aaaatgtcgc
     2701 cgttacgcac tctcacgccg gcgcattcga ccggcacgtc gcgctgcacc atcctggctc
     2761 ggcccttggt atcgagcgga ccgatgccac cgtggaagac cgggaagcgc atctcgcgga
     2821 tctgcttcac gtc
//
LOCUS       DASQOP010000072        16217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_227751, whole genome shotgun sequence.
ACCESSION   DASQOP010000072 DASQOP010000000
VERSION     DASQOP010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 16217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16217
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227751"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..142
                     /locus_tag="VE030_02675"
     CDS             <1..142
                     /locus_tag="VE030_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HZF22351.1"
                     /translation="EMVMPGDNVAITVQLIQPIAMEEGLRFAIREGGRTVGAGVVSKV
                     IE"
     gene            237..312
                     /locus_tag="VE030_02680"
     tRNA            237..312
                     /locus_tag="VE030_02680"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:270..272,aa:Trp,seq:cca)
     gene            424..771
                     /gene="secE"
                     /locus_tag="VE030_02685"
     CDS             424..771
                     /gene="secE"
                     /locus_tag="VE030_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HZF22352.1"
                     /translation="MADKIKLALAALLVIAGVAGFYYLGDRALILRIASVLAGLVAAV
                     LVGWTTEPGRQFYVYAQEAVAETRKVVWPSRKETVQTTGMVLAFVLVMAIFLWIVDAV
                     LVWGVKLLMGPGA"
     gene            771..1307
                     /gene="nusG"
                     /locus_tag="VE030_02690"
     CDS             771..1307
                     /gene="nusG"
                     /locus_tag="VE030_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HZF22353.1"
                     /translation="MSKKWYVVHAYSGFEKSVQRALVERIGRAGMEDKFGQILVPVEE
                     VVEMKSGQKNISERKFFPGYVLVEMDMTDETWHLVKNTPKVTGFVGGAPGRRPPPISE
                     KEVQAILHQIQEGVEKPKPKVLFEVGEAVRVKEGPFTDFHGSVEEVNYDKNKLRVSVS
                     IFGRATPVELDFSQVEKA"
     gene            1464..1895
                     /gene="rplK"
                     /locus_tag="VE030_02695"
     CDS             1464..1895
                     /gene="rplK"
                     /locus_tag="VE030_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012357135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HZF22354.1"
                     /translation="MAKKVVGFVKLQVPAGKANPSPPIGPALGQRGLNIMEFCKAFNA
                     QTQGVEPGLPLPVVITAYADKSFTFIIKTPPTSVLIRKAAKAEKGSAKPNTDKVGRLT
                     RAQVEQIAKQKMPDLTAADLEAAMRTVAGSARSMGVEVEGF"
     gene            1897..2601
                     /gene="rplA"
                     /locus_tag="VE030_02700"
     CDS             1897..2601
                     /gene="rplA"
                     /locus_tag="VE030_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015420223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HZF22355.1"
                     /translation="MTHISKRTKELRAKIDPGKAYPIADAIKLVKEGASAKFNESVDV
                     AINLGIDAKKSDQTVRGSIVLPKGTGKTVRVAVFAQGDKAQAAKDAGADIVGFEDLAA
                     QVKEGKIDFDVAIATPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVADAVKNAKAGQ
                     VQYRADKAGTVQCTIGRASFTEEALKENFLALMDALNKSRPLGTKGIYLKKVSVSSTM
                     GPGVRVDQASLGAQQQ"
     gene            2962..3486
                     /gene="rplJ"
                     /locus_tag="VE030_02705"
     CDS             2962..3486
                     /gene="rplJ"
                     /locus_tag="VE030_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HZF22356.1"
                     /translation="MGLNLDDKKAVVAEVSAKVARAQAIVLAEYRGLEVGDMTALRAK
                     ARSSGVYLRVLKNTLARRAVTGTPFESLAQHMVGPLAYGISADPVAAAKVLNEFAKAN
                     ERFVIKAGAMPGQVMTPKQVATLATMPSREELLAKLMGTMQAPIAKFVRTLNEVPGKF
                     VRTLAAVRDQKQAA"
     gene            3529..3909
                     /gene="rplL"
                     /locus_tag="VE030_02710"
     CDS             3529..3909
                     /gene="rplL"
                     /locus_tag="VE030_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HZF22357.1"
                     /translation="MAMAKAELIDAIGNMSVLELSQLIKDMEEKFGVSAAAAAVAVAA
                     PAGGGAAAAVEEKTDFSVILAATGDNKVNVIKVVRAVTGLGLKEAKDLVDGAPKPVKE
                     GIPKKDAEEIKKQLEEAGAKAEIK"
     gene            4174..8247
                     /gene="rpoB"
                     /locus_tag="VE030_02715"
     CDS             4174..8247
                     /gene="rpoB"
                     /locus_tag="VE030_02715"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HZF22358.1"
                     /translation="MAYSFTEKKRVRKSFAKRASVLQVPYLLATQIDSYAQFLQAELA
                     ADKRKNQGLQAAFQSIYPIVSHSGNARLDFVSYQLGTPPFDVKECQQRGLTYAAPLRA
                     KVRLTIMDKEAAKPTVKEVKEQEVYMGEIPLMTTTGSFVINGTERVIVSQLHRSPGVF
                     FEHDRGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDYLYFRVDRRRKMPVTILLKAIG
                     YTPEQILAGFFMMDTFHISKKGIQFELKPDRMRGEIARFDIVTKQGKLIVQKDKRITV
                     KHVRDMEQAGLSKIAVSEEFLLGRTLGHSLVNKETGEIVANANDEITESLLKKIVEAG
                     IDTIQTIYTNDLDQGPYISQTLRADETTDQMQAQVAIYRMMRPGEPPTEDAVRTLFNG
                     LFYAEERYDLSAVGRMKFNRRVGRNELTGPGTLSNEDIIAVIQILIDLRNGRGEIDDI
                     DHLGNRRVRSVGELAENQFRAGLVRVERAVKERLSQAESENLMPHDLINAKPVSAAVR
                     EFFGSSQLSQFMDQTNPLSEITHKRRVSALGPGGLTRERAGFEVRDVHPTHYGRVCPI
                     ETPEGPNIGLINSLALYARTNEYGFLETPYRKVANGRVTDDIEFLSAIEEGKYVIAQA
                     NAAIDKKGKLLDELVSCRHRNEFTMATVDRVEYMDVAPSQIVSVAASLIPFLEHDDAN
                     RALMGSNMQRQAVPCLRAEKPLVGTGIERTVAVDSGTAVQALRGGVVDYVDASRIVVR
                     VNDEETSAGEVGVDIYNLVKYTRSNQNTNINQRPIVKVGDHIARGDVVADGASTDMGE
                     LALGQNLLVAFMPWNGYNFEDSILISERVVAEDRFTSIHIEELSVVARDTKLGPEEIT
                     RDISNLSENMLARLDESGIVSIGAEVDAGDVLVGKVTPKGETQLTPEEKLLRAIFGEK
                     ASDVKDTSLRVPSGMSGTVIDVQVFTREGIERDKRAQQIIDDELKRYKKDLADQLRIV
                     EDDAFGRIERLLTGKTANGGPKKLAKGAKITKAYLQDIERYHWFDVRLANEEAAQQLE
                     QLQEGLEQTRKAFDAAFEEKKRKLTSGDELPPGVQKMVKVYLAVKRRLQPGDKMAGRH
                     GNKGVISKITPIEDMPHMADGTPVDVVLNPLGVPSRMNVGQILETHLGWAAKGLGKRI
                     GELLKAQAKIADVRKALTLIYNSSGKAEDISDFTDIEVIDLARNLQSGVPFATPVFDG
                     ATEDEIKTMLELAGLPKSGQIALMDGRTGELFDRPVTVGYMHMLKLHHLVDDKMHARS
                     TGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVGGRTKVY
                     ENIVKGEHKIDAGMPESFNVLVKEIRSLAIDIDLDRY"
     gene            8351..12592
                     /gene="rpoC"
                     /locus_tag="VE030_02720"
     CDS             8351..12592
                     /gene="rpoC"
                     /locus_tag="VE030_02720"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HZF22359.1"
                     /translation="MKALLDLFKQVTQEEEFDAIKIGLASPEKIRSWSYGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTLSKVRRERM
                     GHIELASPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAFVVTNPGMTPMKRGDLL
                     SEDAYLEKVEEFGDEFHAAMGAEGVRELLRHLDLNKEIDTLRKELEGTDSETKIKKIA
                     KRLKVLEGFQKSGIKADWMVMEVLPVLPPELRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLELKAPEIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIKGK
                     GGRFRQNLLGKRVDYSGRSVIVVGPQLKLHQCGLPKKMALELFKPFIFHKLEVMGIAG
                     TIKAAKREVENENPVVWDILEEVIREHPVMLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLSSNNILSPANGDPIIVPSQDIVL
                     GLYYMTRQKIGARGEGMIFRDVAEVQRAYDNRQAELHARVLVRIKEFDKDEAGNITER
                     VTRYDTTVGRALLSEILPAGLPFELINKALKKKEISKLINAGFRRCGLRETVIFADKL
                     MYTGFTYATRAGISICVDDMEIPPEKEQLIDAAEKEVQDIESQYTSGLVTQGERYNKV
                     VDIWGRAGDMVAKAMMERLGQEPVVEWDARKNDWAPMKDKKGNPMSQESFNAIYMMAD
                     SGARGSAAQIRQLAGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLAD
                     TALKTANSGYLTRRLVDVTQDLVVIEDDCGTTNGVAMKALIEGGEVVEALRERILGRV
                     AANDVVNPENSQITYPAGSLLDEEAVERIETLGIDEVKVRTPLTCDTRYGLCAKCYGR
                     DLGRGQIVNVGEAVGVIAAQSIGEPGTQLTMRTFHIGGAASRTAVASQIESKSNGTAR
                     FSPLMRYVTNVRGEQVAISRSGEVLILDDNGRERERHKVPYGATLHVADASPVKAGKV
                     LATWDPHTRPIITEYAGRVKFDNVEEGVTVARQIDDVTGLSTLVVIDPKRRGVAQAKG
                     LRPSVKLVDEKGKEVKIPGSELPVNITFQIHSVITVKDGQQVGVGEVLARIPQETSKT
                     RDITGGLPRVAELFEARSPKDAGVLAEVTGTVSFGKDTKGKQRLVITDLDGVAHEYLI
                     PKDKHVMAHDGQVVNKGESIVDGPADPHDILRLLGVEALARYITDEVQDVYRLQGVKI
                     NDKHIEVIVRQMLRRVQIVEVGDSEFITGEQVERSEVLQVNDQLEADGKKPATYEYLL
                     LGITKASLSTDSFISAASFQETTRVLTEAAIMGKKDDLRGLKENVIVGRLIPAGTGLA
                     YHKNRRVRGQPQEFAAEGLIGEPVAAEAAGDEQVA"
     gene            12729..13106
                     /gene="rpsL"
                     /locus_tag="VE030_02725"
     CDS             12729..13106
                     /gene="rpsL"
                     /locus_tag="VE030_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HZF22360.1"
                     /translation="MPTINQLVRKPRAMPKTKSKVPALAGSPQKRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHMVRGSL
                     DTQGVKDRKQSRSKYGAKRPKAA"
     gene            13208..13678
                     /gene="rpsG"
                     /locus_tag="VE030_02730"
     CDS             13208..13678
                     /gene="rpsG"
                     /locus_tag="VE030_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HZF22361.1"
                     /translation="MPRRREVPKRETLPDPKYSSQEVAKFVNVLMTRGKKSIAEAIVY
                     SALEQINKKTGKDPLEVFTQALGNVRPMVEVKSRRVGGANYQVPVEVRSVRRNALAMR
                     WLRDAARKRAEKSMDARLAAELAEAAEGRGGAMKKREEVHRMAEANKAFAHYRF"
     gene            13797..15893
                     /gene="fusA"
                     /locus_tag="VE030_02735"
     CDS             13797..15893
                     /gene="fusA"
                     /locus_tag="VE030_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HZF22362.1"
                     /translation="MPRVTPIERYRNIGISAHIDAGKTTTTERILFYTGVSHKIGEVH
                     DGAAIMDWMEQERERGITITSAATTCFWKGMDGSFPQHRINIIDTPGHVDFTIEVERS
                     MRVLDGACMVYDAVAGVQPQSETVWRQANKYKVPRLAFINKMDRQGADYFKSYDHIRT
                     RLKGNPVPIQIPVGAEEKFEGIVDLVKMKAIYWDDATQGTKYDLREIPANLVEEAKKW
                     REKMVEAAAEANDELMHKYLEGHDLSEADIKAGLRTRVIKNEIVPMLCGSAFKNKGVQ
                     AMLDAVIDYMPSPVDIPPVTGEAEDGKIVSRKADDSEPFAALAFKIMTDPYVGQLTFI
                     RVYSGVLKSGDTVYNSVRGRKERIGRLLQMHANQREEIKEVFAGDIAAAVGLKEASTG
                     ETLCHPDKVITLERMVFPEPVISQAVEPKTKADQEKMGIALNRLAQEDPSFRVHTDEE
                     SGQTIISGMGELHLEIIVDRMKREFGVEANVGKPQVAYREAIKKPVEVEGKFIKQSGG
                     RGQYGHVWLKLEPNEAGKGYEFIDAIKGGTVPREYIPAVDKGLQETLPNGVLAGFPVV
                     DVKVTLFDGSYHDVDSNENAFKMAASIAFKDGMRRANPVLLEPMMAVEVETPENFVGN
                     VIGDLSSRRGMIQGQEEVAGTMVIRAEVPLAEMFGYSTTLRSATQGRATYTMEFKRYA
                     EAPKTVAEAVINKKAA"
     gene            15912..>16217
                     /locus_tag="VE030_02740"
     CDS             15912..>16217
                     /locus_tag="VE030_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZF22363.1"
                     /translation="MSKGKFERTKPHVNVGTIGHVDHGKTTLTAALTLVLSKKFGGEA
                     KAYDQIDNAPEEKARGITINTAHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGA
                     "
BASE COUNT         3573 a         4809 c         5193 g         2642 t
ORIGIN      
        1 cgagatggtg atgcctgggg acaacgtggc cattaccgtg cagctcattc agcccattgc
       61 gatggaagag ggcttgcgct ttgccattcg cgagggcggc cgcaccgtgg gtgcgggcgt
      121 ggtgtcaaag gtcatcgagt aaatcagcga ttaagtgatt cagcgattga gtaatttggc
      181 gattcagtga cttggacaat cacttaaatc accgtttacc taatcacccg aatttaaggc
      241 cagtagctca attggcagag cgtcggtctc caaaaccgaa ggttgggggt tcgagaccct
      301 cctggcctgc cacccgctac gcgagtgatg agtgttgagt gtttaatttt gagttcttgg
      361 tcttaactta acatttaaaa ctcatcactc aacactccgt aggataaaca ctccgaagga
      421 aagatggcag acaagatcaa gttggcgctg gcggcactgc tggtcattgc aggagtcgct
      481 ggcttctatt atctgggtga cagggcgctg attctgcgga ttgcctcggt gctcgcgggg
      541 ttggtcgctg cggtattggt gggatggacg accgagccgg gccggcagtt ctatgtctat
      601 gcccaggagg cggtggccga aaccaggaaa gtggtgtggc cgtcgcgcaa ggaaacggtg
      661 cagaccaccg gaatggtgct cgccttcgtg ctggtgatgg cgatcttcct gtggatcgtg
      721 gacgcggtcc tggtatgggg cgtgaaactc ttgatgggac cgggggcgtg atgtcgaaaa
      781 agtggtatgt ggttcacgcc tactcgggct tcgagaagag cgtccagcgc gcgctggtgg
      841 agcgcatcgg ccgggccggc atggaagaca aattcggcca gatcctggtc ccggtcgaag
      901 aagtggtcga gatgaaaagt ggccagaaga acatcagcga gcgcaagttc ttcccgggtt
      961 acgtgctggt cgaaatggac atgaccgacg agacctggca cctggtgaag aacacgccga
     1021 aagtgaccgg tttcgtcggc ggcgcgcccg ggcgccgtcc gccgccgatc agcgaaaagg
     1081 aagtccaggc gattctgcac cagatccagg aaggcgtgga gaagcccaag cccaaggtgc
     1141 tgttcgaagt gggcgaggcg gtgcgcgtta aggaaggacc gttcaccgat ttccacggtt
     1201 cggtggaaga agtgaactac gacaagaaca agctgcgggt ttcggtttcc atcttcgggc
     1261 gcgccacgcc cgtcgagctg gatttcagcc aggtcgaaaa ggcgtaaacg ggaaatggta
     1321 aatgggaaat ggtaacggct gagcaaaggg taggtttttc gattcactat tcacgattca
     1381 ccatttacgg atttttacgg attttgaatg gggagcgcgc ctctgggcgt gcgctagcac
     1441 ccactctcta gaggagtcat atcgtggcaa agaaagtcgt cggcttcgtc aagctgcagg
     1501 ttcctgcagg caaagccaat ccgagcccgc cgatcggccc ggcactgggc cagcgcggcc
     1561 tcaacatcat ggagttctgc aaggccttca acgcccagac ccagggcgtg gagccgggtc
     1621 tgccgctgcc ggtggtgatc accgcctacg cggacaagag tttcaccttc atcatcaaga
     1681 ccccgccgac ctcggtgctg atccgcaagg cggcaaaggc cgagaagggc agcgccaaac
     1741 cgaataccga caaggtcggc aggctgaccc gcgcgcaagt cgagcagatc gccaagcaga
     1801 agatgcccga tctgaccgct gcggatctgg aagcggcgat gcgtaccgtg gccggtagcg
     1861 cacgcagtat gggcgtcgaa gtggaggggt tctgaaatga cacatatttc caagagaacc
     1921 aaggagttgc gggcaaagat cgaccccggc aaggcgtacc cgatcgctga tgcgatcaaa
     1981 ctggtgaaag aaggcgccag cgccaaattc aacgaatccg tggacgttgc catcaatctc
     2041 ggtatcgacg ccaagaagtc cgatcagacc gtgcgcggct ccatcgtgct gcccaagggc
     2101 accggcaaga cggtacgggt cgcggtgttc gcgcagggcg acaaggcaca ggcggccaag
     2161 gatgccggag ccgacatcgt tggcttcgag gaccttgccg cccaggtaaa ggaaggcaag
     2221 attgacttcg atgtggcgat cgccacgccc gacgcgatgc gtgtggtcgg ccagctcggc
     2281 caggttctcg gcccgcgcgg gctgatgccg aacccgaaag tgggcacggt cacaccgaat
     2341 gtcgcggatg cagtcaagaa cgcaaaggcc ggccaggtcc agtaccgcgc cgacaaggcc
     2401 ggcacggtcc agtgcaccat cggccgcgcc tcgttcaccg aagaggcgct gaaggaaaac
     2461 tttctggcgt tgatggacgc attgaacaaa tcgaggccct tggggactaa gggcatctat
     2521 cttaagaaag tctcggtctc cagcaccatg ggaccgggcg tgcgggtgga ccaggccagc
     2581 ctgggcgccc agcagcagta aggactttgg gaactggcgg cccgcttgtt cgggggcggt
     2641 cagggctgtc aaagaccgtt ggggcccaga aaggtgaagg gtgaagggtg aaaagtgaag
     2701 ggtcaaaccg gaacgtttct ccttcttccc gagcctcacg ggcttaatcg ttcgagccgg
     2761 aaaaggcagt gattgggcgt ggaactcttc acccttcacc cttcacggca agtgcccaac
     2821 gcagatagcg tactcaagag acagatatcg ggcgggcggg aaatcagcgc cggctcctgg
     2881 atcgaaggtc gctatatctg aaagggtgag gcctattacc tcgggtgaaa ggcctcttgt
     2941 tcaactttag gagggtagac cttgggtctc aatctagacg acaagaaagc ggtagtggcg
     3001 gaggtcagcg cgaaggttgc gcgggcccag gccatcgtgc tcgccgaata ccgcggcctc
     3061 gaagtcggcg acatgaccgc gttgcgcgcg aaagcacgca gctcgggcgt gtacctccga
     3121 gttctcaaga acaccctggc gcgccgcgcc gtcaccggca caccgttcga gagcctggct
     3181 cagcacatgg tagggccgct cgcgtacggc atatctgccg atccggtggc tgcggcgaag
     3241 gtactcaatg aatttgccaa ggcgaacgag cggttcgtca tcaaggccgg cgcaatgccc
     3301 ggacaggtca tgaccccgaa acaggtcgcg accctggcca ccatgccgag ccgcgaggaa
     3361 ctgctggcaa aactcatggg cacgatgcag gcgccgatcg ccaagttcgt ccggacactc
     3421 aacgaagtgc cgggcaagtt tgtgcgcacg ctggctgcag tcagggatca gaaacaggcc
     3481 gcgtaacaac cgcgacttaa ccgttttcag gatttcagga gatacgaaat ggcaatggca
     3541 aaagctgaac tcatcgacgc catcggcaac atgagcgtcc tcgagctctc gcaactcatc
     3601 aaggacatgg aagaaaaatt cggggtttcc gcagccgccg cggccgttgc cgtcgctgct
     3661 cccgcgggcg gcggcgcagc tgccgctgtg gaggaaaaga ccgatttttc cgtgattctc
     3721 gccgcgaccg gcgataacaa ggtgaacgtg atcaaggtgg tgcgtgcagt gaccggtctg
     3781 ggtctgaagg aagccaagga cttggtggac ggcgcgccca aaccggtcaa ggaaggcatt
     3841 cccaagaagg acgccgagga gatcaagaag caacttgaag aagccggcgc gaaagccgag
     3901 atcaaataag gcttgcacgg gcatgcaggg aggctggtga ctgcggtcgc cggccttttt
     3961 ctgcataaag gacgaggcaa aagaggctaa agaaaaaccc ccgagaggcg gtttctcttt
     4021 gtcttctgaa gcgactgcag aagacaggta tggtcggatg gcggggttcc ccgccatcgt
     4081 ccgccagcgg ttggtagcgg ccaaccacca cgcccatcac cagtcgatga acgcagcgcg
     4141 cgatccccgc gcctgccgtg aatcggagat gtcatggctt actcctttac cgaaaagaaa
     4201 cgcgttcgca agagctttgc caagcgcgcg agcgtgttgc aggtgccgta cctgctggcg
     4261 acgcagatcg actcgtacgc gcagttcctg caggccgaac tggccgcgga caagcgcaag
     4321 aaccagggcc tgcaggccgc attccagtcg atctatccga tcgtcagcca ttccgggaat
     4381 gcgcggctcg attttgtcag ctaccagctc ggcaccccgc ctttcgacgt caaggagtgc
     4441 cagcagcgcg gcttgactta cgcggcgccg ctgcgcgcca aggtgcggct gaccatcatg
     4501 gacaaggaag ccgccaagcc cacggtgaag gaagtcaagg aacaggaagt gtacatgggc
     4561 gaaatcccgc tcatgaccac caccggctcg ttcgtcatca acggcaccga acgcgtcatc
     4621 gtgtcgcagc tgcaccgctc gccgggcgtg ttcttcgagc acgaccgcgg caagacccac
     4681 tcctccggca agctgctgtt ctcggcgcgc atcatcccct accgcggttc ctggctggac
     4741 ttcgaattcg atccgaagga ctacctgtat ttccgcgttg accggcgccg caagatgccg
     4801 gtcacgatcc tgctcaaggc gatcggctac accccggaac agattcttgc cggcttcttc
     4861 atgatggata ccttccacat atcgaagaag ggcatccagt tcgagctcaa gccggaccgc
     4921 atgcgcggcg aaatcgcgcg cttcgacatc gtcaccaagc agggcaagct gatcgtccag
     4981 aaggacaagc gcatcaccgt caagcacgtg cgcgacatgg agcaggcagg gctgagcaag
     5041 atcgcggtat ccgaagagtt cctgctcggc cgcacactcg gccacagcct ggtcaacaag
     5101 gaaacaggcg agatcgtcgc caacgccaac gacgagatca ccgagagtct gctcaaaaag
     5161 atcgtcgagg cggggatcga cactatccag accatttaca ccaacgacct cgaccaaggg
     5221 ccgtacatct cccagacgct gcgcgccgac gaaaccaccg accagatgca ggcgcaggtg
     5281 gcgatctacc gcatgatgcg ccccggggag ccgcccacgg aagacgcggt gcgcacgctg
     5341 ttcaacgggt tgttctacgc cgaggagcgt tacgatctgt cggcggtggg acggatgaag
     5401 ttcaaccgtc gcgtcggccg caacgaactg acaggcccgg gcacgctgtc caacgaggac
     5461 atcatcgcgg tcatccagat cctgatcgat ctgcgcaacg ggcgcggcga aatcgacgac
     5521 atcgaccacc tcggcaaccg ccgcgtgcgc tcggtgggtg aactcgccga gaaccagttc
     5581 cgcgccggcc tggtgcgggt ggagcgcgcg gtcaaggaac gcctctcgca ggccgaatcc
     5641 gagaacttga tgccgcatga tctgatcaat gccaagccgg tatcggcggc ggttcgcgag
     5701 ttcttcggtt cgtcgcagtt gtcgcagttc atggaccaga ccaatccgct gtcggaaatc
     5761 acccacaagc gccgcgtttc cgcgctggga cccgggggcc ttacgcgtga acgtgccggc
     5821 ttcgaggtgc gcgacgtgca cccgacccac tacggacgcg tgtgtccgat tgaaaccccg
     5881 gaaggcccga acatcggcct gatcaattcg ctggcgctgt acgcccgcac caacgagtac
     5941 ggcttcctgg aaacgcccta tagaaaagtg gcaaacggcc gggtcaccga cgacatcgaa
     6001 ttcctgtcgg cgatcgagga aggcaagtac gtcatcgccc aggccaatgc cgcgatcgac
     6061 aagaagggca agctgctcga cgagctggtg tcctgccgcc atcgcaacga attcacgatg
     6121 gcgactgtcg accgcgtcga gtacatggac gttgcgcctt cgcaaatcgt gtcggtggcc
     6181 gcttcgctga tcccgttcct ggagcacgac gacgccaatc gggcgctgat gggatcgaac
     6241 atgcagcgcc aggccgttcc ctgcctgcgc gcggagaagc cgctcgtcgg caccggcatc
     6301 gaacgcacgg tggcagtgga ctccggcacc gcggtgcagg cgttgcgcgg cggcgtggtc
     6361 gattacgtcg atgcctcgcg catcgtggtg cgggtcaacg acgaagagac ctcggccggc
     6421 gaggtcggcg tggatatcta caacctggtg aagtacaccc gttccaacca gaacaccaac
     6481 atcaaccagc gtccgatcgt gaaagtcggc gaccacatcg cccgcggcga cgtggtggcc
     6541 gacggcgcat caaccgacat gggcgaactg gcgctcggcc agaacttgct ggtggcgttc
     6601 atgccctgga acggctacaa cttcgaggac tccatcctga tctcggagcg cgtggtggcc
     6661 gaagaccggt tcacctcgat ccatatcgag gagctgtcgg tggtggcgcg cgacaccaag
     6721 ctcggacccg aggaaatcac ccgcgacatc tccaatctgt ctgagaacat gctggcgcgg
     6781 ctcgatgagt ccggcatcgt gtctatcggc gcggaagtgg atgcgggcga cgtgctggtg
     6841 ggcaaggtga cgcccaaggg cgaaacccag ctcacgccgg aagagaagct gctgcgcgcg
     6901 atcttcggcg agaaggcctc ggacgtgaag gacaccagct tgcgcgtgcc ctctggcatg
     6961 tccggcacag tgatcgacgt gcaggtattc acccgcgaag gcattgagcg cgacaagcgc
     7021 gcccagcaga tcatcgacga cgaactcaag cgctacaaga aagacctcgc cgaccagttg
     7081 cgcatcgtcg aggacgatgc cttcggccgg atcgagcgct tgctcaccgg caagaccgcc
     7141 aacggtggtc cgaagaagct cgccaaggga gccaagatca ccaaggccta cctgcaggat
     7201 atcgaacgct accactggtt cgacgtgcgt ctggcgaacg aggaggcggc acagcagctc
     7261 gagcagttgc aggagggact ggaacagacc cgcaaggcat tcgatgcggc cttcgaggaa
     7321 aagaagcgca agctcaccag cggcgatgaa ctaccccccg gcgtgcagaa gatggtcaag
     7381 gtttacctcg cggtcaagcg caggctccag cccggcgaca agatggcggg ccgccacggc
     7441 aacaaagggg tgatctccaa gatcacgccg attgaggaca tgccgcacat ggccgacggt
     7501 accccggtgg acgtggtgct caatccgctg ggggtgccgt cgcggatgaa cgtcggccag
     7561 attctggaaa cccatctcgg atgggcggcc aagggtctgg gcaaacgcat cggcgaactg
     7621 ctcaaggccc aggccaagat cgccgacgtc cgcaaggcgc tgacgctgat ctacaactcc
     7681 agcggcaaag ccgaggacat cagcgatttc accgacatcg aggtcatcga tctggcgcgc
     7741 aatctccagt ccggcgtgcc gttcgcgacc ccggttttcg atggtgccac cgaggacgag
     7801 atcaagacca tgctcgagct cgcgggcctg ccgaaatcgg gccagatcgc gctgatggac
     7861 ggccgcaccg gtgaactctt cgaccggccg gttaccgtgg gttacatgca catgctgaag
     7921 ctgcaccacc tggtggacga caagatgcac gcccgttcca ccggcccgta ctcgctcgtc
     7981 acccagcagc cgctgggcgg caaggcgcag ttcggcggcc agcgtttcgg cgaaatggaa
     8041 gtctgggcgc tcgaggctta tggcgctgcg tacacgctgc aggaaatgct caccgtcaaa
     8101 tccgacgacg tgggcggccg caccaaggtg tacgagaaca tcgtcaaggg cgaacacaag
     8161 atcgacgccg gcatgccgga gtccttcaac gtgctggtga aggaaatccg ctcgctcgcg
     8221 atcgacatcg acctcgaccg ctactaacgc gtgaagggtg aatgagtgaa gagtgaaggg
     8281 tcaatgcgta ctaccttcac tgacgatctt tcgacttagg cgaactactc ctcacataac
     8341 gaggttagaa atgaaagcat tgctcgattt gttcaagcag gtcacgcagg aagaagagtt
     8401 cgacgccatc aagatcggac tggcttcccc tgagaaaatc cgttcctggt cgtacggcga
     8461 agtgaagaag cccgagacca tcaactaccg caccttcaag ccggagcgcg acggcctgtt
     8521 ctgcgccaag atctttggcc cggtgaaaga ttacgagtgc ctgtgcggca agtacaagcg
     8581 cctgaagcac cgcggcgtga tctgcgaaaa atgcggcgtg gaagtcaccc tgtccaaagt
     8641 gcgtcgcgag cgcatgggcc acatcgaact ggcgagcccg gtggcgcaca tctggttcct
     8701 gaagagcctg ccgagccgcc tgggcatggt gctcgacatg acgctgcgcg acatcgagcg
     8761 cgtgctctat ttcgaggcat tcgtggtcac caatccgggg atgacgccga tgaagcgcgg
     8821 cgacctgctg tccgaggacg cctatctgga gaaggtcgag gaattcggcg acgaattcca
     8881 tgcggcgatg ggcgccgagg gcgtgcgcga actgctgcgc cacctcgatc tgaacaagga
     8941 aatcgacacg ctgcgcaagg agctcgaggg taccgactcc gagaccaaga tcaagaagat
     9001 cgccaagcgg ctgaaagtgc tcgagggctt ccagaagtcc ggcatcaagg ccgactggat
     9061 ggtgatggaa gtgctgccgg tgctgccgcc cgaactgcgg ccgctggtgc cgctcgatgg
     9121 cgggcgcttc gcgacctccg atctgaatga cctgtaccgc cgcgtcatca accgcaacaa
     9181 ccggctgaag cgcctgctcg aactcaaggc accggaaatc atcgtacgca acgagaaacg
     9241 catgctgcaa gaggcggtgg attcgctgct ggacaacggc cgccgcggca aggcgatgac
     9301 cggcgctaac aaacgtccgc tcaagtcgct cgccgacatg atcaagggca agggcggccg
     9361 cttccgccag aacctgctgg gcaagcgcgt cgactactcc ggccgttcgg tgatcgtggt
     9421 cgggccgcaa ctgaagctgc accagtgcgg actgccgaag aagatggcgc tggagctgtt
     9481 caagcccttc attttccaca agctggaagt gatgggcatc gccggcacca tcaaggcagc
     9541 caagcgcgag gtcgagaacg agaacccggt ggtgtgggac atcctcgaag aagtgatccg
     9601 cgagcacccg gtgatgctga accgtgcccc gacgctgcac cgcctgggca tccaggcttt
     9661 cgagccggtg ctgatcgaag gcaaggcgat ccagctgcac ccgctggttt gcgcggcgtt
     9721 caacgccgac ttcgacggcg accagatggc ggtgcacgtg ccgctgtcgc tggaagccca
     9781 gatggaagcg cgcacgctga tgctgtcctc gaacaacatc ctgtcgccgg cgaacggcga
     9841 cccgatcatc gtgccgtcgc aggacatcgt gctgggcctg tactacatga cgcggcagaa
     9901 gatcggcgcg cgcggcgagg gcatgatttt ccgcgacgtt gccgaggtac agcgcgccta
     9961 cgacaaccgg caggcggaac tgcacgcgcg cgtactggtg cgcatcaagg agttcgacaa
    10021 ggacgaagcc ggcaacatca cagagcgcgt tacccgctat gacaccacgg tgggtcgtgc
    10081 gcttctgtcc gaaatcctgc ctgcaggact gccgttcgag ctgatcaaca aggcgctcaa
    10141 gaagaaggaa atttccaagc tgatcaacgc cggtttccgc cgttgcggcc tgcgcgagac
    10201 ggtcatcttc gccgacaaac tgatgtacac cggctttacc tacgcgaccc gcgccggcat
    10261 ctcgatctgc gtggacgaca tggaaatccc accggagaag gagcaattga tcgacgccgc
    10321 agagaaggaa gtacaggaca tcgagtcgca atacacctcg ggcctggtga cgcagggcga
    10381 acgctacaac aaggtggtgg acatctgggg ccgcgccggc gacatggtcg ccaaggccat
    10441 gatggagcgc ctcggacagg aaccggtggt ggagtgggac gccaggaaga acgactgggc
    10501 gccgatgaag gacaagaagg gcaacccgat gtcgcaggaa tcgttcaacg ccatctacat
    10561 gatggccgat tccggggcgc ggggttcggc tgcgcagatc cgtcagctcg ccggcatgcg
    10621 cggcctgatg gcgaagccgg atggttccat catcgaaacg ccgatcaccg cgaacttccg
    10681 cgaaggcctg aacgtactgc agtacttcat ctcgacgcac ggcgcgcgca agggcttggc
    10741 cgacaccgcg ctgaagaccg cgaactcggg ttatctcacc cgccgtctgg tcgacgtgac
    10801 ccaggacctg gttgtcatcg aagatgattg cggcacgacc aacggtgtgg ccatgaaggc
    10861 cctgatcgaa ggcggcgaag tggtcgaggc tttgcgcgag cgcattctcg gccgcgtggc
    10921 cgccaacgac gtggtcaatc ccgagaacag ccagattacc tacccggccg gctcgctgct
    10981 ggacgaagag gcggtggagc gcatcgagac gctgggtatc gacgaagtca aggtgcgcac
    11041 gccgctcacc tgcgacacgc gttacggtct gtgcgccaag tgttacgggc gcgacctggg
    11101 ccgcgggcag atcgtcaacg tcggcgaagc ggtcggcgtg atcgcggcgc agtcgatcgg
    11161 cgagccgggt acccagctca ccatgcgcac tttccacatc ggcggcgcag cttcgcgtac
    11221 cgcggtggcg agccagatcg aatcgaagtc caacggcacg gcgcgcttct cgccgctgat
    11281 gcgctatgtg accaacgtgc gcggcgaaca ggtggcgatt tcccgttccg gcgaagtgct
    11341 gatcctggac gacaatgggc gcgagcgcga acgccacaag gttccctacg gcgccacgct
    11401 gcatgtggcc gatgcgagcc cggtcaaggc cggcaaggtg ctggccacct gggatccgca
    11461 cacccggccg atcatcaccg agtacgccgg gcgggtgaag ttcgacaacg tggaagaagg
    11521 cgtgacagtg gcgcggcaga tcgatgatgt caccggccta tccacgctgg tcgtcatcga
    11581 tcccaagcgt cgcggcgtgg cgcaagccaa ggggctgcgc ccgagcgtga agctggtcga
    11641 cgaaaagggc aaggaagtga agatcccggg ctccgaactg ccggtgaaca tcacgttcca
    11701 gatccattcc gtgatcaccg tgaaggacgg ccagcaggtg ggcgtcggcg aagtgctggc
    11761 gcgcatcccg caggagactt ccaagacccg cgacatcacc ggcggcctgc cgcgggtggc
    11821 cgagctgttc gaggcgcgtt cgcctaagga tgccggcgtg ctcgcggaag tcactggcac
    11881 cgtgtcgttc ggcaaggaca ccaagggcaa gcagcgcctg gtgatcaccg atctggacgg
    11941 ggtggctcac gaatacctga tccccaagga caagcacgtg atggcgcacg acggccaggt
    12001 ggtgaacaag ggcgagagca tcgtcgacgg cccggccgat ccgcacgaca tcctgcgcct
    12061 gctgggagtc gaggcgctgg ctcgttacat caccgatgaa gtgcaggacg tgtaccgtct
    12121 gcagggcgtg aagatcaacg acaagcacat cgaggtaatc gtgcggcaga tgttgcgccg
    12181 ggtgcagatc gtcgaggtcg gcgattccga gttcatcacc ggcgagcagg tcgagcgctc
    12241 ggaagtgctg caggtgaacg accaactcga agccgatggc aaaaagccgg ccacttacga
    12301 gtacctgctg ctggggatca ccaaggcgtc gctgtcgacc gactcgttca tctcggcagc
    12361 ctcgttccag gagaccaccc gggtactaac cgaggcggcg atcatgggca agaaggacga
    12421 cctgcgtgga ctcaaggaaa acgtcatcgt cggccgactc attcccgccg gcaccggtct
    12481 ggcttaccac aagaaccgcc gcgtccgcgg gcagccgcag gagttcgcgg ccgagggcct
    12541 gatcggcgaa ccggtggcgg cggaggccgc tggagacgag caggttgctt gacaccccag
    12601 acggcggccc atagaatctg ccgtcttttt acctgccgtt ccggcggcaa tcccgggtcg
    12661 gcggtgatag cagcatacaa ccgtgggacg gcggcgacgc cgtcccgggt tttttaggac
    12721 cggaaataat gccaacaatc aaccaattag tgcgcaagcc gcgagccatg ccgaagacca
    12781 aaagcaaggt tccggcgctt gcagggtccc cgcaaaaacg tggcgtttgc acgcgcgtct
    12841 ataccaccac gccgaagaag ccgaactcgg cgctgcggaa agtggccaag gtgcgcctca
    12901 ccaatggttt cgaggtgatc agctacatcg gcggcgaagg ccacaacctg caggagcact
    12961 cggtagtgct gattcgtggc ggccgcgtga aggacctgcc gggcgtgcgc taccacatgg
    13021 tacgcggcag cctcgacacg cagggcgtga aggaccgcaa gcagagccgt tccaagtacg
    13081 gcgctaagcg gcccaaggcc gcctagcgcg gaattttgaa ttttgaatgt tgagtgttga
    13141 gtgaactgca aaggaccgga gccccggggt ttcaactcaa cacttaacac ttaacactgg
    13201 tttaaatatg ccacgacgca gagaagtacc gaagcgcgag acgcttccgg atccgaaata
    13261 cagcagccag gaggttgcca agtttgtcaa cgtgctgatg acccgcggca agaagtcgat
    13321 cgcggaggcc atcgtctaca gcgcactgga gcagatcaac aagaaaaccg gcaaggatcc
    13381 gctggaggtg ttcacccagg cgctcggcaa cgtgcgcccg atggtggagg tcaagagccg
    13441 ccgcgtcggc ggcgccaact accaggtgcc ggtggaagtg cgttcggttc gccgcaacgc
    13501 cctggcgatg cgctggttgc gcgatgcggc gcgcaagcgc gccgagaagt cgatggacgc
    13561 acgcctggcg gccgaactgg ccgaagccgc ggaaggccgc ggcggcgcca tgaaaaagcg
    13621 cgaggaagtg caccgcatgg cggaggccaa caaggccttc gcccattacc gcttttagcg
    13681 gcaatgagag aattttgagt gatgaatttt gaatgttgag tttcggttca accactaaac
    13741 actcaacatt caacactcaa cactcaacac tcaacactta acactggttt actaaagtgc
    13801 cccgcgtcac acccatcgag cgataccgca acatcggcat cagtgcgcac atcgatgccg
    13861 gcaagaccac gaccaccgaa cgcatcctgt tctacaccgg cgtgtcgcac aagatcggcg
    13921 aagtgcacga cggggccgcg atcatggact ggatggagca ggaacgcgag cgcggcatca
    13981 ccatcacttc cgcagcgacg acctgtttct ggaaggggat ggacggcagc tttccgcagc
    14041 accgcatcaa catcatcgac acgcccggcc acgtggattt caccatcgaa gtcgagcgct
    14101 ccatgcgcgt gctggacggc gcctgcatgg tctacgatgc cgttgccggc gtgcagccgc
    14161 aatcggaaac cgtgtggcgc caggccaaca agtacaaggt accgcgcctg gcattcatca
    14221 acaagatgga tcgccagggc gcggactact tcaagtccta cgaccatatc cgcacacgcc
    14281 tcaagggcaa cccggtgccg atccagattc cggtcggcgc agaagagaag ttcgagggca
    14341 tcgtcgacct ggtcaagatg aaggcgatct actgggacga cgcgacgcag ggcacgaagt
    14401 acgaccttcg cgaaattccg gccaatctgg tcgaagaggc caagaaatgg cgcgagaaaa
    14461 tggtcgaggc cgcggccgaa gccaacgacg aactgatgca caagtacctc gagggacacg
    14521 acctctcgga ggcggacatc aaggcaggcc tgcgcacgcg cgtcatcaag aacgaaatcg
    14581 tgccgatgct gtgcggctcg gcattcaaga acaagggcgt gcaggcgatg ctcgacgcgg
    14641 tgatcgacta catgccctcg ccggtggaca tcccgccggt tacgggagaa gccgaggacg
    14701 gcaagatcgt gtcgcgcaag gccgacgaca gcgagccgtt cgccgcgctg gcgttcaaga
    14761 tcatgaccga cccctatgtc ggccagctca ccttcatacg cgtctattcg ggcgtgctca
    14821 agtcgggcga caccgtctac aactcggtgc gcgggcgcaa ggagcgcatt ggccgtctgc
    14881 tgcagatgca cgccaaccag cgcgaagaaa tcaaggaagt gttcgccggc gacatcgccg
    14941 ccgcggtcgg cctcaaggag gcttcgaccg gcgaaacgct ctgccatccc gacaaggtca
    15001 tcacgctcga gcgcatggtg ttccccgagc cggtcatttc ccaggcggtg gaacccaaga
    15061 ccaaggccga ccaggaaaag atgggcattg cactcaaccg cctggcgcag gaagatcctt
    15121 cgttccgcgt ccacaccgac gaggaatccg ggcagaccat catctccggg atgggcgagt
    15181 tgcacctgga aatcatcgtc gaccgcatga agcgcgagtt cggtgtcgag gccaacgtcg
    15241 gcaagccgca ggtcgcttac cgcgaagcca tcaagaagcc ggtggaagtg gaaggcaagt
    15301 tcatcaagca gtccggcggc cgcggccagt acggccacgt ctggctcaag ctggagccga
    15361 acgaggcggg caagggatac gaattcatcg acgccatcaa gggcggcacg gtgccgcgcg
    15421 aatacatccc agccgtggac aagggcctgc aggaaacgct gcccaacggc gtgctggccg
    15481 gattccccgt ggtggacgta aaggtcacgc tgttcgacgg ttcctatcac gacgtggact
    15541 cgaacgaaaa cgcgttcaag atggccgcct cgatcgcctt caaggacggc atgcgcaggg
    15601 ccaacccggt cctgctcgaa ccgatgatgg cggtggaagt ggagacgcca gaaaacttcg
    15661 tcggcaacgt catcggcgat ctgtcttcgc gccgcggcat gatccagggc caggaggaag
    15721 tggcgggcac gatggtgatc agggcggaag tgccgctggc ggaaatgttc ggctattcga
    15781 cgacgctgcg ctccgccacc cagggccgcg ccacatatac gatggaattc aagcgttacg
    15841 ccgaagcgcc gaagactgtc gccgaggcgg tgatcaataa gaaggctgcg taagcaggaa
    15901 ggaacggcaa catgtcaaag ggaaagtttg agcgcaccaa gccgcacgtg aacgtgggga
    15961 cgattggtca cgtggaccat ggcaagacca cgctgacggc ggcgctgacg ctggtgctgt
    16021 cgaagaagtt cgggggcgag gcgaaggcct acgaccagat cgacaatgcg ccggaagaaa
    16081 aggcgcgcgg gatcacgatc aacactgcgc acgtggaata cgagaccgcc aagcgccact
    16141 acgcgcacgt ggactgcccg gggcatgccg actacgtgaa gaacatgatc acgggggcgg
    16201 cgcagatgga cggggcg
//
LOCUS       DASQOP010000073         5330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2040709, whole genome shotgun sequence.
ACCESSION   DASQOP010000073 DASQOP010000000
VERSION     DASQOP010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2040709"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1293)
                     /gene="secY"
                     /locus_tag="VE030_02745"
     CDS             complement(<1..1293)
                     /gene="secY"
                     /locus_tag="VE030_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HZF22364.1"
                     /translation="MAENSFLGLSGKFGDLKRRLLFLLGALIVYRIGAHVPVPGIDPV
                     QLAALFKSQQGGILDMFNMFSGGALSRFTIFALGIMPYISASIIMQLLTVVSPQLEQL
                     KKEGESGRRKITQYTRYGTLFLATFQALSIAIALESQRGLVLEPGLAFRFITVVTLVT
                     GTMFLMWLGEQITERGIGNGISLIIFAGIAAGFPRAVGGTLELARTGAFSIPLVLLLL
                     VGAVLVTGLVVFVERGQRKILVNYAKRQVGRKVYGGQSSHLPLKLNMAGVIPPIFASS
                     IILFPATLAGWFGSNEGMTWLRDISGKLSPGQPIYVLLYASAIVFFCFFYTALVFNPK
                     ETAENLKKGGAFVPGIRPGEQTAKYIEKIIMRLTLSGAIYVTLVCLLPEFLIVKWNVP
                     FYFGGTSLLIIVVVTMDFMAQVQAYVMSHQYESLLKKAN"
     gene            complement(1301..1735)
                     /gene="rplO"
                     /locus_tag="VE030_02750"
     CDS             complement(1301..1735)
                     /gene="rplO"
                     /locus_tag="VE030_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008642954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HZF22365.1"
                     /translation="MRLNQIKPAAGSKHAKKRVGRGVGSGTGKTAGRGHKGQKARSGG
                     FHKIGFEGGQMPLHRRLPKRGFVSLTSGDTVEVRLSAINELPGDDIDILVLKQAGVVP
                     NAARAAKVILAGEIKRKVKLRGLLVTKGARAAIEKAGGVIEG"
     gene            complement(1736..1903)
                     /gene="rpmD"
                     /locus_tag="VE030_02755"
     CDS             complement(1736..1903)
                     /gene="rpmD"
                     /locus_tag="VE030_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HZF22366.1"
                     /translation="MRVTLVRGLIGTRESHRATVRGLGLRRINHTVELEDTPAVRGMI
                     NKVSYLLKCEA"
     gene            complement(2066..2578)
                     /gene="rpsE"
                     /locus_tag="VE030_02760"
     CDS             complement(2066..2578)
                     /gene="rpsE"
                     /locus_tag="VE030_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008642951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HZF22367.1"
                     /translation="MARMQQRTEERSDGLREKMVSVNRVTKVVKGGRILGFAALTVVG
                     DGDGGIGMGKGKAREVPVAVQKAMDEARRKLVKVSLKNGTLQHAVVGQHGAAKVFMQP
                     ASEGTGIIAGGPMRAIFEVMGVTNVLAKCIGSTNPYNVVRATINGLQGMSTASEIAAK
                     RGKTVEEILG"
     gene            complement(2590..2943)
                     /gene="rplR"
                     /locus_tag="VE030_02765"
     CDS             complement(2590..2943)
                     /gene="rplR"
                     /locus_tag="VE030_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HZF22368.1"
                     /translation="MIDKKQSRMRRARQTRAKIAEMKAVRLAIYRSNGHIYAQVIDPA
                     AKVLATASTLEPEVRKQVSNGANVKAAAVVGKRIAEKARAQGVEQVAFDRAGFKYHGR
                     VKALAEAAREAGLKF"
     gene            complement(2956..3489)
                     /gene="rplF"
                     /locus_tag="VE030_02770"
     CDS             complement(2956..3489)
                     /gene="rplF"
                     /locus_tag="VE030_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HZF22369.1"
                     /translation="MSRIGNLPVTLPSGVEVTLAPGQISVKGPLGVLSQRLGAGVEVK
                     RDGDKVVFKRLDDSIQSNALSGTLRALVANMVQGVTKGFERKLQLVGVGYRAQAQGDK
                     LNLTVGFSHPVVHKMPKGIKVETPQQTEIVIKGIDKQVVGQVAAEVRGYRPPEPYKGK
                     GVRYADERVVLKETKKK"
     gene            complement(3512..3907)
                     /gene="rpsH"
                     /locus_tag="VE030_02775"
     CDS             complement(3512..3907)
                     /gene="rpsH"
                     /locus_tag="VE030_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HZF22370.1"
                     /translation="MSMSDPIADMLTRIRNAQLAEKTSVAVPASKLKVSIAMVLKDEG
                     YIEDFALRGEGAKRALEIGLKYYAGRPVIERIERVSRPGLRIYKPANDIPQVMNGLGV
                     AIVSTSRGVMTDRKARQTGVGGEVLCIVA"
     gene            complement(3921..4226)
                     /gene="rpsN"
                     /locus_tag="VE030_02780"
     CDS             complement(3921..4226)
                     /gene="rpsN"
                     /locus_tag="VE030_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HZF22371.1"
                     /translation="MAKTALVNREKKRRATVKKFAARRAELLAIINNSRVSDEDRTQA
                     REKFQQLPRNSSPVRLRNRCSLTGRPRGVFSKFGLGRSKLREIAMRGEIPGVTKASW"
     gene            complement(4234..4764)
                     /gene="rplE"
                     /locus_tag="VE030_02785"
     CDS             complement(4234..4764)
                     /gene="rplE"
                     /locus_tag="VE030_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015420243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HZF22372.1"
                     /translation="MQQHFREAVVPALMKQFGYKTAMQVPRIDKIVLNMGVGEAVNDK
                     KILDNAVGDMTRIAGQKPVVTKAKKSIAGFKIRKDYPIGCKVTLRRSRMYEFLDRLVS
                     VAIPRIRDFRGIAARSFDGRGNFNLGVKEQIIFPEIEYDKIDALRGLNITITTTAKSD
                     EEARALLAAFKFPFKS"
     gene            complement(4942..5259)
                     /gene="rplX"
                     /locus_tag="VE030_02790"
     CDS             complement(4942..5259)
                     /gene="rplX"
                     /locus_tag="VE030_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HZF22373.1"
                     /translation="MRKIRKGDNVVVITGKDKGKRGTVLRVLGDDHLLVEGANRVKKH
                     QKPNPMKGTTGGIIDKEMPIHVSNVAVFNPTTQKADRIGVKQMDDGRRVRIYKSNGEM
                     LDA"
BASE COUNT          858 a         1816 c         1523 g         1133 t
ORIGIN      
        1 gttcgccttc ttgagcaggc tctcgtactg gtgcgacatc acgtaggcct gcacctgggc
       61 catgaaatcc atcgtcacca ccacgatgat cagcaggctg gtgccgccga agtagaacgg
      121 cacgttccac ttcacgatca gaaactccgg cagcaggcac accagcgtca cgtagatcgc
      181 gcccgacagc gtcagccgca tgatgatctt ctctatgtac ttcgccgtct gctcccccgg
      241 ccggatcccc ggcacgaaag ccccgccttt cttcaggttc tccgccgttt ccttggggtt
      301 gaacaccaag gcggtgtaga agaagcagaa gaacacgatc gcggaggcgt acaacaacac
      361 gtaaatcggc tgcccggggg atagcttgcc gctgatgtcc cgcaaccagg tcatgccttc
      421 attgctcccg aaccagccgg ccagcgtggc cgggaacagg atgatgctcg aggcaaaaat
      481 cggcgggatc accccggcca tgttcaattt cagcggcaga tgcgaactct gcccgccata
      541 aactttgcga ccgacctggc gcttggcgta gttgaccagg atcttgcgct ggccgcgttc
      601 gacgaacacc accagtccgg tcaccagcac cgctcccacc agcagcagca gcaccagcgg
      661 gatcgagaac gccccggtgc gcgccagttc cagggtgccg ccgaccgcgc gcggaaatcc
      721 cgccgcgatt cccgcgaaga taatcagcga aatgccgttg ccgatgccgc gttcggtgat
      781 ctgttcgccg agccacatca ggaacatcgt gccggtcacc agcgtcacca cggtaatgaa
      841 acggaacgca agtcccggct ccagcaccag gccgcgttgc gattccagcg caatggcaat
      901 gctcagcgcc tgaaaagtcg ccaggaacaa cgtgccatag cgcgtgtact gggtgatctt
      961 cctgcgtccc gactcgccct ccttcttcag ctgctccagc tgcggcgata ccaccgtcag
     1021 cagctgcatg atgatcgacg ccgagatata cggcatgatc cccagcgcaa agatggtgaa
     1081 ccgcgacagc gcgccgcccg agaacatgtt gaacatgtcc aggatgccgc cctgctgcga
     1141 cttgaacagc gccgccagtt gcacgggatc gatgcccggc accggcacgt gcgcgccgat
     1201 ccgatagacg atcagcgcgc caagcaggaa cagaagccgg cgcttcaggt cgccgaactt
     1261 cccggacagt cccagaaatg agttctctgc caagttctat tcagccttca atgacgccgc
     1321 cggccttctc gatggccgca cgcgcgcctt tggtgaccag caatccgcgc agtttcacct
     1381 tgcgcttgat ctcgccggcc aggatgacct tggccgcgcg cgccgcgttc ggcactacgc
     1441 cggcctgctt cagcaccagg atgtcgatgt cgtcccccgg caactcattg atcgccgaca
     1501 aacgcacctc gacggtatcg ccgctggtca acgaaacgaa gccgcgtttg ggcaggcgcc
     1561 ggtgcaacgg catctgaccg ccctcgaagc cgatcttgtg gaaaccgccg gagcgcgcct
     1621 tctgcccctt gtggccgcgg cctgcggtct taccggtgcc gctgccgacg ccgcgaccga
     1681 cccgtttctt ggcgtgtttg gagccggctg ccggtttaat ctggttcaaa cgcatttacg
     1741 cctcgcactt cagcaggtag ctcaccttgt tgatcatgcc gcgcaccgcc ggggtgtctt
     1801 ccagttccac cgtgtggttg atgcgccgca ggcccaggcc acgcaccgtg gcacgatggc
     1861 tctcgcgggt accgatcagg ccgcgcacca gcgtgactct cactttcttt ccggatttcg
     1921 cggccgcttt atccgcgggt ttcctgaaat gcgagtcatc tttcgtggcg gctgttttcc
     1981 cgaccggcgc attgcctgcc gcttgcttgc cgggtgcttt ttccccgggg gcctttttct
     2041 cagtcactat gttctcaggc atggcttacc ccaggatctc ttctacggtc ttgccgcgct
     2101 tggccgcgat ctcggaggcg gtgctcatcc cctgcagacc gttgatcgtg gcgcgaacca
     2161 cgttgtaggg attggtcgaa ccgatgcact tggcgagcac gttggtcacg cccatcacct
     2221 cgaagatcgc ccgcattgga ccgccggcga tgatccccgt gccctcggaa gcgggctgca
     2281 tgaacacttt ggcggcgccg tgctggccga ctaccgcgtg ctgcagggtc ccgttcttca
     2341 gcgatacctt gaccaacttg cggcgcgctt cgtccatcgc cttctgcacc gccaccggca
     2401 cttcgcgggc tttgcccttg cccatgccga tgccgccatc gccgtcgccc accaccgtca
     2461 gcgcagcaaa gccgaggatg cggccgccct tcaccacttt ggtgacgcga ttgaccgaca
     2521 ccatcttttc gcgcaggccg tcgctgcgct cttccgtacg ttgttgcatt ctggccatgg
     2581 tctaatcctt cagaacttga ggccggcttc acgcgccgct tcggccagcg ctttcacgcg
     2641 gccgtgatat ttgaatccgg cgcggtcgaa ggccacctgt tccacgccct gcgctcttgc
     2701 cttttcggcg atgcgcttgc ccaccaccgc agccgcttta acgttggcgc cgttgctcac
     2761 ctgtttgcgc acctccggct cgagcgtcga ggcggtcgcc agcaccttcg cggccgggtc
     2821 gatcacctgc gcatagatgt ggccgttgga gcggtaaatt gccagccgca ccgccttcat
     2881 ttcggcgatc ttggcgcggg tctggcgggc gcgccgcatg cgtgactgct tcttgtcgat
     2941 catgtccggt cccgtttact tcttcttggt ctctttgagc acaacgcgct cgtcggcgta
     3001 gcgcacgccc ttgcccttgt agggctcggg cggacgataa ccgcgtactt ccgccgcaac
     3061 ttgtccgacc acctgcttgt cgatgccctt gatcacgatt tcagtctgct gcggcgtttc
     3121 gaccttgatg cccttgggca tcttgtgaac caccggatgc gagaaaccca ccgtcaggtt
     3181 gagcttgtcg ccctgcgcct gcgcccggta gcccacgccg accagctgca gttttctctc
     3241 gaagcccttg gtcacgccct gcaccatgtt ggccaccagc gcccgcagcg tcccggacag
     3301 cgcgttcgac tggatcgaat cgtcgagccg cttgaatacc accttgtcgc cgtcgcgttt
     3361 cacttccacg ccggcaccga ggcgttgcga caacacgccc agcggcccct tgaccgaaat
     3421 ctggccaggg gccaaggtca cttccacccc gctgggaaga gtgaccggaa gatttccaat
     3481 gcgagacatc tgttctgctc ctcgcccgcg gctacgccac gatgcacaac acttcgccgc
     3541 cgacgccagt ctggcgcgcc ttgcggtcgg tcatcacacc cctggaggtg gacacgatcg
     3601 ccacgcccaa cccgttcatc acctgcggaa tgtcattggc cggcttgtaa atgcgcagcc
     3661 ccggacggct gacccgctcg atgcgctcga tcaccggccg gccggcgtag tacttcaagc
     3721 cgatctcgag cgcccgtttg gcgccctccc cgcgcagcgc gaagtcctcg atatagcctt
     3781 cgtccttgag gaccatggcg atcgacacct tgagcttgct cgcgggcacg gcaaccgagg
     3841 ttttctccgc aagctgcgcg tttcgtatcc gcgtcagcat gtcggcaatg ggatcactca
     3901 tgctcatcca aagtctcctg ctaccagctt gccttggtca cgccgggaat ctcgccacgc
     3961 atggcgatct cgcgcagctt gctgcggccc agaccgaact tgctgaacac cccgcgcgga
     4021 cgccccgtca gcgagcagcg attgcgcagc cgcaccgggc tggagttgcg cggaagctgc
     4081 tgaaacttct cgcgcgcttg cgtgcggtcc tcgtccgaaa ccctgctgtt gttgatgatt
     4141 gcgagcagct cggcgcgcct ggccgcgaat ttcttcacgg tggcgcggcg tttcttttcg
     4201 cgattgacca atgcggtctt tgccatggcg acctcagctc ttgaacggga acttgaaggc
     4261 ggcgaggagc gcacgcgctt cctcgtcgga cttcgctgtc gtggtgatgg tgatgttcag
     4321 gccccgcagt gcatcgatct tgtcgtactc gatctcggga aaaatgatct gttccttcac
     4381 gcccaggttg aaattgccgc ggccgtcgaa ggagcgcgcg gcaatgccgc ggaagtcgcg
     4441 gatgcgggga atcgccaccg agaccagccg gtccaggaat tcatacatgc ggctgcggcg
     4501 caacgtcacc ttgcagccga tcgggtagtc cttgcggatc ttgaagccgg cgatcgattt
     4561 cttggccttg gtcaccaccg gcttctggcc ggcgatcctg gtcatgtcgc ccaccgcgtt
     4621 gtccaggatc ttcttgtcgt tgaccgcctc gcccacaccc atgttcagca cgatcttgtc
     4681 gatgcgcggc acctgcatgg cggtcttgta cccgaactgc ttcatcaggg ccggcaccac
     4741 cgcttcgcgg aaatgctgct gcagccgcgc cggttgaacc ggtccggcct tctccgctgc
     4801 gctcttcgcg gatgcggcct tcgcgggcgc tgctttcttg gcctttgccg tatcttcctt
     4861 cttcggcgtg tcttccttga ccttcgccgt gtcttccttg gccttcgccg tatcttcctt
     4921 ggcctttgcc atgtcttcct cttaagcgtc cagcatttcg ccgttcgact tgtagatccg
     4981 cacgcggcgg ccgtcgtcca tctgctttac gccgatgcgg tcggctttct gggtggtcgg
     5041 gttgaacacc gccacgttgg aaacgtggat cggcatctcc ttgtcgatga tgccgccggt
     5101 ggtgcccttc atcggattgg gcttctggtg cttcttgacg cggttggcac cctcgaccag
     5161 gagatggtca tcgccgagca cgcgcagcac ggtgccgcgc ttgcccttgt ccttgccggt
     5221 gatgaccacg acattgtcgc ccttgcggat cttgcgcatg attgttcctt acaacacttc
     5281 cggcgcgagc gagacgatct tcatgaatcg ctcggttcgc agttcgcgcg
//
LOCUS       DASQOP010000074        21069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2447608, whole genome shotgun sequence.
ACCESSION   DASQOP010000074 DASQOP010000000
VERSION     DASQOP010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 21069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21069
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2447608"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2404)
                     /locus_tag="VE030_02795"
     CDS             complement(<1..2404)
                     /locus_tag="VE030_02795"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZF22374.1"
                     /translation="MSRQLKILIVEDVPNDAELALRELKRAGIAFDARRVDTAAEYRR
                     ELEQFKPELILADFSMPNFDGAEALVIARNHFPDIPFIFVSGSIGEEDAIRAMKQGAN
                     DYVMKTNLVRLSAAVERAIQEQRERTARRDAEYALRASELRKSAIFEAALDSIVTIDS
                     QGRVIEFNPAAEKTFGYTREEVLGKELAELVVPPASREAHRRGLKHYNATGEGPILGK
                     RIEVSALRKDGSEFPVELTVVPIRLPDQTLFSAYISDITERKRIEEEVRETRNFLASI
                     FDNIPNMIFVKDAGELRFVRLNPAGEKLIGYSENELLGKNDYDFFPKDQADFFVARDR
                     ETLSSGERLFVVEETITAKDGTRKILQTKKLPILGKDGSPRYLLGFSEDITERKEAEL
                     RLRESEERFRQVAENIREVFWLTDPSKGEMLYISPTYEAIWGRSRQSLYAVPRAWAEA
                     IHLDDRDRVLKAAQTRQMAGEYVEEYRIVRPDGTMRWIRDRAFPVTDTDGIVIRIAGI
                     AEDITERKEQEHRIARLSRIHTVLSGINSAIVRIRDRQELFKESCRIAVEHGGFSIGW
                     IAIMDHASKTLVPVAQMGLPMDLGGRGDSSNRPAGLVPGGTAEVALREKRPAFDNDIE
                     HGLGEMQSEPGSDTMGVRRAAIRLGAKSVIVLPLYVEGKTFGILTLYAPERNFFDDEE
                     IKLLTELAGDISFGLEFISKEEEVNYLAYYDVLTKLPNRTFLEDRLGQYLHAAQRGQR
                     MAAVMLIDLERFHVVNDTLGRNAADEVLREIASRLQVQIRAEDTVARVGADCFAIALS
                     DAS"
     gene            complement(2814..2999)
                     /locus_tag="VE030_02800"
     CDS             complement(2814..2999)
                     /locus_tag="VE030_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22375.1"
                     /translation="MELIRRGDPQVMNLDIRLKDSSGFDKATEFKRGDQGADRSGVCA
                     AHFGFDKPAKPRVLINE"
     gene            complement(3133..3777)
                     /locus_tag="VE030_02805"
     CDS             complement(3133..3777)
                     /locus_tag="VE030_02805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZF22376.1"
                     /translation="MTIRVLIADDHGVVAEGLRNLVEAQADMKVVALVEDGREAVRSA
                     MEHSPDVVLMDVAMPVLNGTEATRIIRERCPQTRVIMLSAYSDPVHVYRALQAGAAGY
                     IAKKSVAKEVVDAIRAAHEGRHYLSGQLNERLIDHVIRKTASDDPILRLSSRERQVLQ
                     MLAEGHSIGEIAVTLSLSPKTVETYRARMMDKLDIRDLANLVRFAIRQGITSLE"
     gene            complement(3779..6142)
                     /locus_tag="VE030_02810"
     CDS             complement(3779..6142)
                     /locus_tag="VE030_02810"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZF22377.1"
                     /translation="MTTFATEQQTRAALLDNERRVLERIAGGAPLWEILETLVKLIEE
                     QADGMRCAVLLADTMQQRLRFAAAPNIPEDYKAGIEPFLRIAPNMGSCGTAAFLRKPV
                     YTKDTGVDALWEDCRDIAVRNGLRAIWSTPILSDDNAVLGTFAMYYGEPRLPSTDHIH
                     LIDMATQMVRVAIEAKSNEELLRTVFEGAPCGMAITDLAGNIVRVNPTFARLVGHTQA
                     ELQGKTIADVTHEEDYPQNKALIDELLAGKREAFVIDKRYRRKDGQLVWVHNTVALVR
                     GPADEPRYMVALIEDISARKRAAEASLQMAGEIQALARQKEDLLRLVIDTIPTMAWSL
                     LPDGAVEFVNQRWLDYTGLSLEEALEDAIHIVHPEDLPSVMEKWSVDMAAGETCEYEM
                     RLRRADGEYRWFLVRTVPLRDEQGKIVKWYGTSTDIEDRKRAEDALRESEEKFRQLAE
                     NIREVVWMTTPAMDELLYVSPAYEVIWARSRESLRQRPRSFMEAIHTKDREHVVGILE
                     RQREQGFEVEYRIVRPDGSVRWIRNRGFPIKNRSGKVYRIAGVAEDITQRKHAGDALR
                     RSAAEHQALSRRLVELQESERRQLSRELHDRVGQNLTALRINLDILQTALASSGSGEV
                     RARVDDSAALLESTMDTIENVMSELRPPMLDDHGLAAALDWHASKFSERTGIAVAVTG
                     SEPAVRPAPQVEIALYRIAQEALNNVAKHARARHVEIALDHANGVCVMSVQDDGIGFD
                     RVEATSDKPKPGLGMVTMRERAQAVGGRFEVQALPGRGTRLTVRVPR"
     gene            complement(6139..6768)
                     /locus_tag="VE030_02815"
     CDS             complement(6139..6768)
                     /locus_tag="VE030_02815"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZF22378.1"
                     /translation="MKVPRTKHRKPISSRPASARERIDQTAYELFSRDGIRAVGVDTV
                     VAHSGVTKMTLYRHYPSKDKLALAFLRRREELWTRAWLQEEVERRARTPGGRLLAVFD
                     AFDKWFRRSDFEGCSFVKALLEQSDLRHPVRHAAVCHIETVRALLRQWGENAGVRNPD
                     RFARLWQILMMGSIIAACAGDLDAAKRAREVGALLLAREGLALAKRLRT"
     gene            complement(6947..7099)
                     /locus_tag="VE030_02820"
     CDS             complement(6947..7099)
                     /locus_tag="VE030_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22379.1"
                     /translation="MAPGSNQWSLPCEHRASHYAGQENADVKQFKSLLAGEIIKAGII
                     LGRQLR"
     gene            complement(7123..7824)
                     /gene="dnaQ"
                     /locus_tag="VE030_02825"
     CDS             complement(7123..7824)
                     /gene="dnaQ"
                     /locus_tag="VE030_02825"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HZF22380.1"
                     /translation="MRQIILDTETTGLDPSLGHRIIEIAAVEIVNRRFTDRHYHQYVN
                     PEREIEAGAIDVHGITGEFLQDKPKMKETAKEFLDFVAGAELIIHNAPFDVGFLDHEL
                     ALLELQPIGHYCPLITDTLKMAKGLHPGKRNSLDALCERYQVDHSARSLHGALLDARL
                     LAEVYLAMTRGQESLLMDLGESSSPALDGAFADEKLELIVLRATSDELAAHAQQLDDI
                     DKAAKGACLWKKLEA"
     gene            complement(8012..8452)
                     /gene="rnhA"
                     /locus_tag="VE030_02830"
     CDS             complement(8012..8452)
                     /gene="rnhA"
                     /locus_tag="VE030_02830"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HZF22381.1"
                     /translation="MSEVVDIYTDGACKGNPGPGGWGVWLKWGDHEKELFGGEPETTN
                     NRMELTAVIEALASLNRRCKVRLHTDSQYVHNGISAWIHDWKKRGWRTADRKPVKNVD
                     LWMKLDELVQKHDVEWIWVRGHHGNPGNERADELANRGVAAELR"
     gene            complement(8449..9225)
                     /locus_tag="VE030_02835"
     CDS             complement(8449..9225)
                     /locus_tag="VE030_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZF22382.1"
                     /translation="MSTRPASSGLSAWLSTPLGAHLLEAELRYFDQELADVFGFNAFQ
                     LGLPEFDFLRANRMPYRWVTGMDGPVRLQSDLCALPIQSAVADLVVLPHALEFSSNPH
                     QVLREVSRILMPEGHVVLSGFNPWSLWGVRRLVSHREGVYPWCGQFINLPRIKDWMAL
                     LGFELAGGRMCCYAPPLTTEKWFRRFDFMEAAGDRWWPFAGGIYFLHGIKRVQGMRII
                     TPKWKSAEVKKKALVAVPHKTNGHDDALAARTNHMAGDPQ"
     gene            9254..10024
                     /gene="gloB"
                     /locus_tag="VE030_02840"
     CDS             9254..10024
                     /gene="gloB"
                     /locus_tag="VE030_02840"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HZF22383.1"
                     /translation="MKMTTIAPIRAFKDNYIWCLRRGTNAAVVDPGDAGPVFDYLRAE
                     GLQLTAILNTHHHADHVGGNVDLLSKYNVPVFGPAHESIPGVTRKLVEGDEIEVPGIG
                     VSLHVLDIPGHTAGHIACYGEGVLFCGDTLFSCGCGRLFEGTPEQMHSSLSKLADLPS
                     ETLVYCGHEYTLANLRFADAAEPGNPALAERLVAARAALDRGTPTLPSTIGTERATNP
                     FLRVREPALVTSASRYAGRTLTNAVEVFAALRKWKDGF"
     gene            10514..11680
                     /locus_tag="VE030_02845"
     CDS             10514..11680
                     /locus_tag="VE030_02845"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HZF22384.1"
                     /translation="MPEIESALVARHEAWYLNHPDYTQLMIERSRLYLYYIVEEVQKR
                     GMPTEVALLPMIESGYNPGAYSRAHAAGIWQFIPSTGRRYGLQQNWWYDGRRDVLTAT
                     RAALDYLQQLHAQFGDWYLALAAYNWGENGVTRAIAQNRAKRKPTDYRSLHMPRETRN
                     YVPKLQAVKNIIVNPQGVGFPLEDIPDQPYFTTVTTPEHIDMQVAARLAEMSEDDFKS
                     LNPGHTRPVITSNGSRTLLLPIDKAGIFNANLKDYDQPLVSWQTYTLKKHETLEQIAE
                     QFGIRTARLKEINGINRHLTVPAGRTLLVPMNEETQSSNLTDTWDNPEFLAPDDYYMN
                     HMVYRVKSGDSLYSIARANRITVAALKDWNRLRSNHIHPGQRLVVYSNPRAARR"
     gene            complement(11970..14018)
                     /locus_tag="VE030_02850"
     CDS             complement(11970..14018)
                     /locus_tag="VE030_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZF22385.1"
                     /translation="MADTLLEVEDLSTWLAANEGMVRAVDGLSFSIERGETFALLGES
                     GSGKSMTALSITRLLPDAGRIVSGAVRLEGEELLQLPEAAMRRVRGRRIGMIFQEPGA
                     SLNPVMTAGAQIAEVLERHTALRGAEVNARVIELLNAVGIPDPAGRVHEYPFQLSGGM
                     KQRVMIAIALACEPSLLIADEPTTALDVTIQAQVLDLLRSLQKRMGMSILLITHDLGV
                     VAEMAHHVAVMYAGEIVEMAPREAFFGNPSHPYSRKLFASVPNRQARDKTLAVIRGTV
                     PSLAGEFHGCRFADRCDYAWDLCRETIPAWAPIAEGHGARCHLYAAGTRDEGRGTRES
                     QEPLNLLPPCFPSPLAPRPSSLSSTTASNLLEVVDLKVHFPIRKGLFKRVVGQVKAVD
                     GVSLSIRAGRTLALVGESGCGKTTVGKGILRLLPLSGGRVRYGGVDLASLPANELRMR
                     RKHLQIIFQDPYSSLNPRMRTMDIVEEGMAALGVGGDAQVRAKRIDDLLGQVGLSREM
                     KYRYPHAFSGGQRQRIAIARALAVEPQLIVCDEPTSALDVSVQAQILNLLKDLQDRLG
                     IAYLFITHNMSVVEYLAHEVAVMYLGRIVEQGTVDDVLGSPKHPYTRALLSAVPSVDP
                     ATRREIIRLQGDLPSPVNPPSGCHFHPRCPQAMPECRQTYPGTSTLSATHTTRCHLYK
                     "
     gene            complement(14025..15479)
                     /locus_tag="VE030_02855"
     CDS             complement(14025..15479)
                     /locus_tag="VE030_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZF22386.1"
                     /translation="MPVVLWTDALVYLLVAVILVFAWHVRRHEHLLAPWRKVAQSRSG
                     MVAAVVLAVFVAVGLLDSLHFRPRLADGAAGGKASYAVEMLSLFDVAAAHLRTRPEKT
                     YSAPLATHLFAKETVELPDGRQARLFPRLTYGGAHLDDPETDWAWDVAKRAIAGVAAA
                     ILVWNLLVLIATIWLSRRDNRGMSDAWRQIWNGESAVPWREMLITACVLLVVLAPMLL
                     LSVKYHVLGTDKVGQDVFYQALKSIRTGLVIGTLTTMVMLPFALLLGIMAGYLRGWVD
                     DVIQYVYTTLNSIPGILLIAASVLMLQVLMDKHAELFETVQQRADAKLLVLCLILGVT
                     SWTGLCRLLRGESLKLREMEYIQAAQAFGVSGFRIITRHILPNVMHIVLIAVVMDFSG
                     LVLAEAVLSYVGVGVDPTMISFGTMINSARLEMAREPMVWWSLAAAFVFMLMLVLSAN
                     LFADAVRDAFDPRIRGRLATPAFISKYLPAVKNS"
     gene            complement(15497..16477)
                     /locus_tag="VE030_02860"
     CDS             complement(15497..16477)
                     /locus_tag="VE030_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZF22387.1"
                     /translation="MINYLIRRVLYAIPILVGVNVITFALFFFVNTPDDMARMQLGQK
                     RVTEEAVRKWKEERGYDRPLFVNSAAQGTGKLTDTIFFTKSVKMFAFDFGRAEADGRD
                     IGHEIRSRMWPSFAIALPVFLVGMLTYITVALLMAFFRGGYIDFAGVVLCVVAMSVSG
                     LFYIIGGQYLISKLWNLVPISGYGEGLSAWKFLVLPVIIGIIAGVGSSARWYRTIFLE
                     EINRDYVRTARAKGLSELRVLFKHVLHNAMIPILTGAVVAIPLLFLGSLITESFFAIP
                     GLGSYTIDAINNQDFSVVRAMVFLGSVLYIVGLILTDFSYTIADPRVRLQ"
     gene            complement(16482..18653)
                     /locus_tag="VE030_02865"
     CDS             complement(16482..18653)
                     /locus_tag="VE030_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZF22388.1"
                     /translation="MSRLSATAVALALLGFLGGCGGPWNNPYPVRERGQNILYSAFVE
                     RPKHLDPVQSYAENEYVFIAQIYMPPLQYHYLRRPYELVPFGAAEIPRAVYLDADGRR
                     LPDSAPAERVAFSVYDIRIRQGVMYQPHPAFAVGESGKPRYLNLGREDLRNIFELRDF
                     GYSGTRELVAEDYVFQIRRLAHPGLHSPIFGLMSEYIVGLKEFAQQLQEAAKHLPADA
                     YLDLTTFPLEGARTLDRYTYRIKIRGKYPQFVYWLAMPFFAPIPEEAHRFYSQPGMTE
                     GKNLTLDWYPVGTGPYMLTVNNPNRLMVMERNPNFAGEPYPADGEAGDAEAGLLDDAG
                     KLMPFIDKVIFSLEKESIPYWNKFLQGYYDYSGISSDTFDQVVQITGAGDIALTEDIQ
                     LRRIALQTSVATSTLYMAFNMLDPVVGGNSERARKLRHAISIALDFEEMISIFRNGRG
                     VAAQGPIPPGIFGYRDGREGMNPYVYDWVEGRPTRKPIAYAQQLLAEAGYANGIEAST
                     GKPLVIYFDGTLVGAEGKSRADWLVKQLAKINLQLVVRNTDYNRFQEKVRKGTAQLFF
                     FGWNADYPDPENFLFMFHGPQSRALKNGENSSNYMNAEYDRLFERVRAMDNGPERQAL
                     IDRMIEMLRFDAPWAWGLHPQDFTLAHAWVHNRKPNKMANNSLKYQRIDSQLRETMRA
                     QWNSPVLWPVLAALAALVLALIPAVLSYRRRERAGGKLAAV"
     gene            18798..19583
                     /gene="fabI"
                     /locus_tag="VE030_02870"
     CDS             18798..19583
                     /gene="fabI"
                     /locus_tag="VE030_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HZF22389.1"
                     /translation="MGFLDKKKILITGLLSNRSIAYGIAKAMHREGALLAFSFQGERV
                     RDRVVELAAEFGSRLVLPCDVASDAEIDALFAELARHWDGLDGIIHSIAYAPREALAG
                     DYLDSVTREAFRIAHEISSYSFAALAKAGLPMMQGRKAALLTLTYLGAERSFPSYNVM
                     GLAKASLEANVRYMAQSLGPKGIRVNAISAGPIKTLAAAGINSFGKLLAYAEKQSPLR
                     RNVSTEEVGNVAAFLCSDLASGITGEITYVDAGFNTTGAGQIE"
     gene            complement(19803..>21069)
                     /locus_tag="VE030_02875"
     CDS             complement(19803..>21069)
                     /locus_tag="VE030_02875"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HZF22390.1"
                     /translation="KYYEEHKNEFQIPERVRLEYLVLSPDAVEHAAKVTDEELKNYYQ
                     ANIERYRTPEQRRASHILIPASAGASAEDKAKAMTLAEDLLKQIRSAPGKFAELATKF
                     SKDPGSAQKGGDLGFFARGLMVKPFDAAVFALKIGELAGPVETQYGYHIIRLDAIKAS
                     QTTPFDAVKQQIVDEVRKPKLGKAYAEAADSFNNLVYEQFDSLQPAAEALKLTVQKSD
                     WVSRTGGNPNPLFNNEKLLHAVFSDEVLKNKHNTSAIEVQPNMLLAARVIEHKPAAAL
                     PLEQVRSDVMQYLTDKGAIELAGKEGRAALEKLQQGQPAALSWSPSQSVSLQKRQGLH
                     PEGAQAVFGADVSKLPVYAGVPAAQGRFVVYRVTKVKDVTAVDTEQRKALGKQLAEMV
                     GQEQYVAYLASLRERADVKVDRKRLEQGQ"
BASE COUNT         4064 a         6687 c         6198 g         4120 t
ORIGIN      
        1 tgctcgcatc cgacaacgcg attgcaaaac aatccgcccc gactcgcgcg accgtgtcct
       61 cggcccggat ttgcacttgc aggcggctgg cgatttctct gagcacttcg tccgcagcgt
      121 ttctgcccag cgtgtcgttg acgacgtgaa accgctccag gtcgatcagc atgaccgccg
      181 ccatgcgctg cccgcgctgc gcggcgtgca agtactggcc gaggcgatct tccagaaacg
      241 tgcggttggg taacttggtg agcacatcgt agtaggcgag gtaattcacc tcctcttcct
      301 tcgagatgaa ctctaatccg aacgaaatgt cccccgccag ctcggtgagc agcttgatct
      361 cctcgtcgtc gaagaaattg cgttccggtg catacaaggt aagaatcccg aacgtcttcc
      421 cctccacgta aagcggcagc acgatgaccg actttgcgcc cagccgaatg gccgctcgcc
      481 tgacacccat tgtgtctgac ccaggctcgc tctgcatttc acccagccca tgctcgatgt
      541 cgttgtcgaa ggccggccgc ttctcgcgta gtgcgacctc cgcggtgccc cccggaacca
      601 gccccgccgg acgattggaa gagtcgccgc ggccacccaa atccattggc agacccattt
      661 gcgcaaccgg cacgagcgtt ttgcttgcgt ggtccattat ggcgatccac ccgatgctga
      721 atccgccatg ctccacggcg atgcggcagg actccttgaa cagctcctgg cgatcacgga
      781 tacgcacgat cgccgaattg atgccgctaa gtacggtatg gatccggctc aatcgcgcga
      841 tcctgtgttc ttgctccttg cgttcggtga tatcctccgc gatcccagcg atacggataa
      901 cgatcccatc cgtgtcggtg acggggaatg cccggtcacg tatccacctc atcgtgccgt
      961 cgggtcgtac gatgcggtac tcttcaacat attccccggc catctgccgc gtctgcgcgg
     1021 ccttcaaaac ccggtcgcgg tcatcgagat ggattgcctc cgcccaggcc cgaggcaccg
     1081 cgtagaggct ttggcgactc cggccccata ttgcctcgta tgtgggactg atgtagagca
     1141 tttcgccctt cgatgggtcg gtcagccaga atacttcgcg gatattctcg gccacctggc
     1201 ggaatcgctc ctcgctctcc cgcaaccgca gctccgcctc cttgcgctcg gtgatgtcct
     1261 ccgaaaaacc cagcaggtag cggggactgc cgtctttgcc gagaatgggc agtttcttgg
     1321 tctgaagtat tttcctggtc ccgtctttgg ctgttatcgt ttcctcgacg acgaagaggc
     1381 gctcaccgct tgacagggtc tcgcgatccc ttgcaacgaa gaagtcggcc tggtccttcg
     1441 ggaagaaatc gtagtcgttc tttcccagca gttcgttctc cgaataaccg atcaactttt
     1501 cgccggcggg gttcaggcgc acgaaccgga gttccccagc atccttcacg aagatcatgt
     1561 tcgggatatt gtcgaaaatg gaagcaagga agttccgggt ctcccggact tcctcctcga
     1621 tccgtttgcg ttcggttatg tcgctgatgt aggcactgaa gagcgtttgg tccggaagcc
     1681 ggatgggcac cacggtgagt tctacgggaa actcgctgcc gtcctttcgc agcgcagaaa
     1741 cttctatgcg tttgcccagt atcggcccct ccccggtggc gttgtagtgc ttaagccccc
     1801 gccgatgggc ttcgcgcgaa gcgggcggca cgacgagctc ggcgagctct tttcccaaca
     1861 cttcttcgcg cgtgtaaccg aaggtcttct ccgccgccgg attgaattcg atgacacggc
     1921 cttgggagtc tatggtgacg atactatcta gcgccgcttc gaaaatcgca ctcttgcgca
     1981 gttcgctggc cctgagggcg tattccgcgt ctctgcgggc cgtgcgttcc cgctgttcct
     2041 gaatcgcccg ctccaccgcg gcggataggc gaaccagatt ggtcttcatg acatagtcgt
     2101 tcgcgccctg tttcatggca cgaatcgcat cctcttcacc gatcgaaccc gagacgaaaa
     2161 tgaacggaat atccgggaaa tggttgcgcg cgatgacaag ggcttccgcg ccgtcaaaat
     2221 tcggcatgga gaagtctgca aggatcaact caggtttgaa ttgttccagt tcgcgacgat
     2281 attcggcagc ggtgtccacc cggcgcgcgt cgaaggcgat gcccgcgcgc ttgagttccc
     2341 gcagcgccag ttcggcgtcg ttcggaacgt cctcaacgat caggatcttg agttgcctgc
     2401 tcatgaagtc ttccgggaag acacccttcg aatcgtgtgc ggaatcgctt ttcaacgcga
     2461 cggtttctgc acggggtgaa ttccggatgc tgattcgaag atcaggctgg tgggcacacc
     2521 ccgggcgacg cgtcgggaag cggaagaaaa aacgagatgg cggttcgccg gcagaactga
     2581 cgtcggtaag tgcgccctgc agaatccggc tgtcgcgagg ggaggaacgc gaaccagtcg
     2641 tcgggcgctc tgagtcagtt gcggaaagcc agggagggga aagacacccg tcatctcgcc
     2701 tgaaaagcta cgcttgcggg cggcagaacc caactttagt tttgccgcca tgacacactg
     2761 ttctccctta aagttgtgtt cgctttccgt aacgagcggt tgtttgtgaa ttattattca
     2821 ttgatcagta cccggggttt ggccggtttg tcgaatccga agtgcgcagc gcaaactcca
     2881 gaccggtcag caccttgatc gcccctcttg aactccgtgg ccttgtcgaa accgctgctg
     2941 tccttgaggc ggatgtccag attcatcact tgcgggtcgc cgcgcctgat cagctccaac
     3001 ccgcctcgtt cgcgagatca gcgaatagag cgcaagtgtt cagcctgaac tctaacgcta
     3061 aagacctacg gatgcagtcg cgtacagccg acaaactacg tcagatattt cctgacagca
     3121 cctgcgtcgc ggtcattcga gagaagtaat tccctgccgg atcgcgaatc tgacaagatt
     3181 cgcaaggtcg cgaatgtcga gtttgtccat cattcgggcg cggtaggtct ccaccgtttt
     3241 gggtgaaagc gacagcgtta ccgcgatttc gccaatagag tgtccctcgg cgagcatttg
     3301 caggacttgt cgctcgcggg agctaagccg cagaattggg tcgtccgagg cagttttgcg
     3361 gatgacgtgg tcgatcagtc tttcattcaa ttgtccgctg agatagtgcc tgccttcgtg
     3421 cgccgctcga atcgcatcga ccacttcctt ggccaccgat ttcttcgcga tatagcctgc
     3481 ggcgccagcc tgtagtgcac ggtacacgtg aaccgggtcc gagtacgcgg agagcatgat
     3541 gacgcgggtc tgagggcacc gctcccggat gatccgcgtc gcctcggtgc cgttcagaac
     3601 gggcatggca acgtccatga ggaccacatc cggactgtgc tccattgcac tgcgcacggc
     3661 ctcgcggccg tcctcgacta gggcgacgac tttcatgtcg gcctgcgcct cgaccagatt
     3721 gcgcaatccc tcagccacga cgccgtgatc gtcggcgatc agaaccctga tcgtcatgtc
     3781 agcgtggaac ccgcacggtg agtcgcgtcc cacggccggg cagcgcctga acctcaaaac
     3841 gcccaccgac cgcttgcgcg cgctctctca tcgtcaccat gccgagtccg ggcttcggtt
     3901 tgtccgaagt agcctccacg cggtcgaagc cgattccgtc gtcctgcacg gacatcacgc
     3961 atactccgtt cgcatgatcg agcgctattt cgacgtgccg cgcgcgggcg tgttttgcga
     4021 cgttgttgag cgcttcctgc gcgatgcgat agagcgcaat ttcgacctga ggtgccggcc
     4081 gtacagccgg ctcgcttccg gttaccgcga ccgcgatccc cgttcgctcg gagaatttac
     4141 tcgcgtgcca gtccaaggcc gccgcaaggc cgtggtcgtc gagcatcggc ggtcgcagct
     4201 cggacatgac gttctcgatc gtgtccatcg tcgactcgag aagcgccgcc gaatcgtcca
     4261 ctcgtgcccg aacctcgccg cttccactcg aagcgagtgc cgtctggagg atgtcgaggt
     4321 tgattctcag ggcggtcagg ttctggccga cccgatcgtg gagctcgcgg gaaagctgcc
     4381 ggcgctcaga ttcctggagc tcgaccagcc gtcgtgacag ggcctggtgc tccgccgccg
     4441 atcgccgcaa ggcgtcccca gcgtgcttgc gctgggtgat gtcctccgca acgccagcga
     4501 tccggtacac ctttccagac cgatttttta tgggaaatcc gcggttacga atccatcgca
     4561 cagacccgtc aggtcttacg atgcggtatt ccacctcaaa tccctgttcc cgctgccttt
     4621 caaggatgcc aaccacatgc tcgcgatcct tagtgtgaat cgcctccatg aacgatcgcg
     4681 gccgttgacg caggctttcc cgagatcgtg cccagatgac ctcatacgcg ggactcacgt
     4741 agagcagctc gtccatagcg ggtgtggtca tccagacgac ctcccggatg ttttcggcga
     4801 gttggcggaa cttttcctcg ctctcgcgca gtgcatcctc ggcccgcttt cggtcctcga
     4861 tatcggtact cgttccatac cacttgacga tctttccctg ctcgtcgcgc agcggcacgg
     4921 tgcggaccag gaaccagcga tactccccgt cggcgcggcg caaacgcatt tcatactcgc
     4981 acgtctcccc agcggccatg tccacagacc acttctccat gacgcttggg aggtcttctg
     5041 gatgaactat gtgaatcgca tcttcaagtg cctcctccaa tgagaggccc gtataatcga
     5101 gccagcgctg attgacgaat tcaacggcgc cgtcaggcag aaggctccag gccatcgtcg
     5161 gaattgtgtc gatgacgagc cgaaggagat cttccttttg ccgcgccaac gcttgaatct
     5221 ccccggccat ctgcagcgac gcctctgccg cgcgcttgcg cgcgctaatg tcttcgatga
     5281 gtgcgaccat gtagcgaggc tcgtcggccg ggccgcgcac gagcgccacg gtattgtgca
     5341 cccagactaa ttggccgtcc tttcggcgat agcgcttgtc gatcacgaag gcctcgcgct
     5401 tacccgcgag cagctcatcg atcagcgcct tgttttgcgg atagtcttcc tcgtgggtga
     5461 cgtcagcgat ggtcttgcct tgaagctccg cttgcgtgtg ccccaccagc cgcgcaaacg
     5521 taggattgac ccggacgata ttccccgcga ggtcggtgat tgccataccg cacggcgccc
     5581 cctcgaaaac cgtccgcagc aactcctcgt tgctcttcgc ctcgatcgcc acacgcacca
     5641 tctgcgtggc catgtcaatc aggtgaatgt gatccgtcga cggtaagcgc ggctcgccgt
     5701 aatacatcgc aaatgtcccg agtacagcgt tgtcgtccga aaggatcggc gtggaccaga
     5761 tcgcgcgcag tccgttgcgg accgcgatgt cgcggcagtc ctcccaaagc gcatcaaccc
     5821 cggtgtcttt cgtatacacc ggtttgcgca ggaacgccgc tgtcccgcac gagcccatgt
     5881 tgggcgcgat gcgcaggaat ggttcgatgc ccgccttgta atcctcgggg atgtttggcg
     5941 cggcggcaaa cctgagccgc tgttgcatgg tgtccgcaag cagcacggcg cagcgcatac
     6001 cgtcagcctg ctcctcgatc agcttgacca gtgtctcgag aatctcccat agtggcgcgc
     6061 cacccgcgat ccgctcgagc acgcggcgct cgttatcaag caacgcggct cgcgtttgtt
     6121 gctcggtagc aaatgtcgtc atgtccgaag tcgcttcgca agcgcgagac cctcgcgagc
     6181 gagaagcagc gcccccactt ccctggctcg cttcgcggca tccagatcac ccgcgcaagc
     6241 ggcgatgatc gatcccatca tcagaatttg ccacagtctt gcgaagcgat ccggattgcg
     6301 tacacctgcg ttctcacccc attgtctcag gagtgcgcgg accgtttcga tgtggcagac
     6361 cgccgcatgc cgaacggggt gccgcaggtc actctgttcg agcagcgctt tcacgaaaga
     6421 acaaccttcg aaatcggacc ggcggaacca cttgtcgaat gcatcgaaca ccgcaagcag
     6481 gcgtcccccg ggggttcggg cccggcgttc cacctcttcc tgcagccaag cccgcgtcca
     6541 caattcctca cgacggcgga ggaaagcaag cgcgagcttg tctttggaag gatagtgccg
     6601 atacaacgtc attttcgtca cgcctgagtg cgcaacgaca gtatcaacgc ccaccgcacg
     6661 gatgccgtct cgggaaaaaa gctcgtaggc agtctgatcg atgcgctcgc gcgcgctggc
     6721 cggacgagac gagattggtt ttcgatgttt ggttcgaggt actttcatac gagagagccg
     6781 tataccattc gacaggtcat gcccgcatat ttgggaccta gacgaccgga taattattta
     6841 tgtttctata cagctctgtc gcttcaatat ctctctaccg gcgattccaa gtctaccgcc
     6901 agtcatatac ggctctgtct ctcacatcca gatcgtattg tcaagttcat ctgagctggc
     6961 gccccaaaat aatgccggcc ttgatgatct cgccagcgag cagcgacttg aactgcttca
     7021 cgtcggcgtt ttcctgaccg gcgtagtgcg aagctcgatg ttcacacggc agggaccact
     7081 ggttcgatcc cggggccacc cgcccggctt tcctgctttt ccctacgcct ccagcttctt
     7141 ccacaagcag gcgcccttgg cagccttgtc gatgtcgtcg agttgctgtg catgggcggc
     7201 aagttcatcc gacgtcgcac gcagcacgat caattccagc ttctcgtcgg cgaacgcgcc
     7261 atccagtgcc ggcgaggacg attcgccaag atccatcagc aggctttcct ggccgcgggt
     7321 catcgccaga tagacttcgg ccagcaggcg cgcatccagc agcgcgccgt gcagcgaccg
     7381 cgcggagtgg tcgacctggt aacgctcgca cagcgcgtcc agcgagttgc gtttgcccgg
     7441 atgcaggcct ttggccatct tcagcgtatc ggtgatcagt ggacagtagt ggccgatcgg
     7501 ctgcagttcg agcagcgcca gttcgtgatc gaggaaaccc acgtcgaacg gcgcgttgtg
     7561 gataatcagc tcggccccgg cgacgaaatc gaggaactcc ttcgccgtct ctttcatctt
     7621 cggcttgtcc tgaaggaact cgccggtgat cccgtgcacg tcgattgctc ccgcttcgat
     7681 ctcccgctcg ggattgacgt actggtgata gtgccggtcg gtgaagcggc gattgacgat
     7741 ctccaccgct gcgatttcga tgatgcgatg cccgagcgac ggatccaggc ccgtagtttc
     7801 agtatcaagg atgatctggc gcatgaaatt caggggagag ggacgagggt ggagggacga
     7861 ggggaaaacc tggacaaggg cgcaaagatt ccaagacgcg aagcagactt catctcgccg
     7921 gtcttccctc gtttctctcc gctcttctct cgtccctcat ccctcgcccc tcgtccctgc
     7981 acctcacccc tcatccctca tccctctccc ctcagcgcag ctcagcggcg acgccgcgat
     8041 tcgcgagctc atccgcgcgt tcgttgccgg gattgccgtg gtggccgcgc acccatatcc
     8101 actcgacgtc gtgcttctgg accagttcgt ccagcttcat ccagagatcg acgttcttga
     8161 ccggtttcct gtccgcggtg cgccagccgc gctttttcca gtcgtgaatc caggcggaga
     8221 tgccgttgtg cacgtactgg gaatcggtgt gcaggcgaac cttgcaacgc cggttcagcg
     8281 atgccagcgc ctcgatcacc gcggtcagct ccatgcggtt gttggtggtc tcgggctcgc
     8341 cgccgaacaa ttccttctcg tgatcccccc acttcagcca gacgccccag ccacccggcc
     8401 cgggattgcc cttgcaggcg ccgtcggtgt aaatgtcgac gacttcgctc attgagggtc
     8461 gccggccatg tgattggtgc gcgccgccag cgcgtcgtca tggccgttgg tcttgtgcgg
     8521 caccgcgacc agcgcttttt tcttcacctc cgcggatttc catttcggcg tgatgatgcg
     8581 catgccctgc acgcgtttga tgccgtgcag gaagtagatc ccgccggcaa acggccacca
     8641 gcggtcgccc gccgcttcca tgaagtcgaa acggcgaaac catttttccg tggtgagcgg
     8701 cggcgcatag cagcacatgc gcccgcccgc gagctcgaag cccagcaacg ccatccagtc
     8761 cttgatccgc ggcagattga tgaactggcc gcaccacgga taaacgcctt cccggtggct
     8821 caccaggcgc cgcacacccc acaggctcca gggattgaat ccggacagga ctacatgccc
     8881 ttcgggcatg aggatgcgcg aaacctcgcg cagcacctga tgcggattac tgctgaattc
     8941 gagcgcgtgt ggcagcacca ccagatcggc aaccgccgac tggatcggca gcgcgcacag
     9001 atccgactgc aaacgcaccg gcccgtccat accggtcacc cagcgatacg gcatgcggtt
     9061 ggcgcgcagg aaatcgaact ccggaagccc gagctggaac gcgttgaatc cgaacacgtc
     9121 ggccagctcc tggtcgaaat agcgcagctc ggcttcgagc aggtgcgccc cgagcggcgt
     9181 ggacagccat gcggacaggc cggaggatgc aggccgggtt gacattcagt attgggccac
     9241 gaaagatacg gccatgaaaa tgaccactat tgctccaatc cgcgcgttca aggacaacta
     9301 tatctggtgc ctgcgacgcg gcaccaatgc cgcagtcgtg gatcccggcg atgccgggcc
     9361 cgtgttcgat tacctgcgcg ccgagggcct gcaacttacc gcgatactca atacccatca
     9421 ccacgcagat catgtcggcg gcaacgtcga tctcctgtcg aagtataacg taccggtatt
     9481 cgggccggcg cacgagagca ttcccggcgt cacccgaaag ctcgtggaag gcgacgaaat
     9541 cgaagtgccg ggcatcggcg taagcctgca cgtactcgac atcccaggcc ataccgcggg
     9601 gcacattgcc tgttacggcg agggggtgtt gttctgcggc gacaccctgt tctcatgcgg
     9661 ctgcgggcgc ctgttcgagg gcacaccgga gcagatgcat tcgtcgctga gcaagctggc
     9721 cgatctgccc tccgaaaccc tggtgtactg cggacacgag tacacgcttg ccaatctgcg
     9781 ctttgccgat gccgcggaac cgggaaatcc cgccctcgcg gaacgactgg tcgccgcacg
     9841 cgcggcactc gatcgcggca cgcccactct gccctcgacc attgggaccg aacgggccac
     9901 caatcctttc ctgcgcgtcc gcgagccggc cctggtcact tcggcgagcc gctatgcggg
     9961 acgcacgctc acaaacgcgg tcgaagtttt cgcagcgttg cggaaatgga aagatgggtt
    10021 ttaagagcag tgaagggtga agagtgaagc gtgaagggtg aaaggcaaaa aacgaggact
    10081 cgggacgagg ggcgagggcc gagaaagtgc ataagtgcgt tcgccaagct ttagtgtgat
    10141 tttccactcg cccctcgtcc ctcgcccctc ctcccttaat ttcttgcgtt gcgggggcgc
    10201 gttcgttagc atacgtcgct gaatccatta agaacggatg gggatgaaaa aaccacgcgc
    10261 gctttttgcc ggcctgtttg ctgcacttgt cgcattcccg gtcttcgcca tcgaaccccc
    10321 tccgcgaaac ccgtccgttt cggaagaacc accggaagat gtctctccgg taatccacga
    10381 agaagtcccg gcagcatcag ccactgaaag cctgcgatcg gacgtgccgg tcgatgcgca
    10441 cgccggtgcc gtggtagcgc cagcatcgca atctgcggga agcctttggg agcgcatacg
    10501 ctccggcttc gccatgcccg aaatcgaaag cgcactggtc gcaaggcacg aagcctggta
    10561 cctgaaccat cccgactata cccagctcat gatcgagcgc agccgtctat acctctacta
    10621 catcgtcgag gaggtgcaaa agcgcggcat gcctaccgag gtcgcgctgc tgccgatgat
    10681 cgagagcggc tacaaccccg gggcttattc gcgcgcgcac gcggccggca tctggcagtt
    10741 catcccctcg accggcaggc gttacggcct gcaacagaac tggtggtacg acggcaggcg
    10801 cgacgtgctc acagccacgc gcgccgccct cgactacctg cagcagttgc acgcgcagtt
    10861 cggcgactgg tacctggcgc tggctgctta caattggggc gagaacggcg tcacgcgggc
    10921 gattgcccag aaccgcgcaa aacgcaagcc gacagactat cgcagcctgc acatgccgcg
    10981 cgagacccgt aactacgtac ccaagctgca ggcagtgaag aacatcatcg tcaatccaca
    11041 gggtgtggga ttcccgctcg aagatattcc ggaccagccc tacttcacca ccgtcacgac
    11101 gcccgagcac atcgacatgc aggtggcggc gcggctcgcg gaaatgtccg aggatgactt
    11161 caagtcgctc aaccccggac acacgcggcc ggtgatcacg tccaacgggt cacgcacgct
    11221 gctgctgccg atcgacaagg ccggcatctt caatgccaat ctgaaggact acgaccagcc
    11281 gctggtgtcg tggcagacat acaccctgaa aaagcacgag acgctggagc agatagccga
    11341 gcaattcgga atccgcactg cgcgactgaa ggaaatcaac ggcatcaacc gccatttgac
    11401 agtgcctgca gggcgtacgc tgctggttcc gatgaacgag gaaacgcaat cctcaaatct
    11461 tacggatacc tgggacaacc cggaattcct ggcgcccgac gactactaca tgaaccacat
    11521 ggtgtaccgg gtgaaaagcg gcgacagcct gtattcgatc gcccgggcga accgcatcac
    11581 cgtggccgct ctcaaggact ggaaccgtct gcgcagcaat cacatccacc caggccagcg
    11641 cctggtcgtc tacagcaatc ctcgcgccgc acggcgctag cagcgcagtt gaaagccggg
    11701 ggtcaaaaac acaggcgcac cacgaaggca cgaagacacg aagaaaagcg aaaaaaagca
    11761 cacgtgtgta aaaagctcca attggcggat gttccaacaa agtccaggtt ggcgtaagag
    11821 gatcacgaac agtcgcctcg cttctatccc cgattaattt aaggattctt gtttttcttc
    11881 gtgccttcgt gtcttcgtgg tgagattttc gcttttgtct ttccgcaccg tttagcaaat
    11941 tccctgctgg cgctccttgc cgtcgtcttc tacttgtaaa ggtggcagcg cgtcgtgtgc
    12001 gttgccgaca aagtgctcgt tcccggatag gtctgccggc attccggcat cgcctgcggg
    12061 cagcgcggat ggaagtgaca gcccgaggga ggattgaccg gtgaaggcag gtcaccctgc
    12121 agccggatga tttcgcgccg ggttgccgga tccaccgaag gcaccgccga caatagcgcg
    12181 cgggtatagg gatgcttcgg gctgccgagc acgtcatcca ccgtcccctg ttcgacgatg
    12241 cggcccaggt acatcacggc cacctcgtgg gcgagatatt cgaccaccga catgttgtgc
    12301 gtgataaaca ggtaggcgat gcccaggcga tcctgcagat ctttcagcag attcagaatc
    12361 tgcgcttgca ccgagacatc gagcgcgctg gtgggttcgt cgcagacgat cagttgcggc
    12421 tcgacggcaa gcgcccgggc gatggcgatt cgctgtcgct gcccgccgga aaacgcgtgc
    12481 gggtagcggt acttcatctc ccgggagagg ccgacctgcc cgaggagatc gtcaatgcgt
    12541 ttggcgcgga cctgcgcgtc accgcccacg cccaacgccg ccatcccctc ttcgacgatg
    12601 tccatggtcc gcatgcgcgg attgagcgag ctgtagggat cctggaagat gatctgcagg
    12661 tgtttgcggc gcatgcgcaa ctcgttcgcc ggcagcgacg ccaggtccac gccgccgtag
    12721 cgcacccttc cccccgacag cggcaaaagc cgcaggatgc ccttgccgac ggtagtttta
    12781 ccgcaaccgg actcgccgac cagcgccagc gtgcgtcctg cccggatcga caacgacaca
    12841 ccgtcgacgg ccttcacttg accaacgacg cgcttgaaca atcctttgcg gatcgggaaa
    12901 tggactttca agtcgaccac ttcgagcaga ttgctcgcag tggtcgacga cagggacgag
    12961 ggacgagggg cgaggggcga gggaaagcaa ggaggaagta gattcaaagg ctcctgactc
    13021 tccctcgtcc ctcgcccctc gtccctcgtc cctgcagcat acaaatggca cctcgctcca
    13081 tgcccttccg cgatcggcgc ccacgccggg atcgtttcgc ggcataggtc ccacgcatag
    13141 tcgcaacgat cggcgaatcg gcaaccgtgg aattcgccgg cgagcgaagg cactgtgccg
    13201 cggatcaccg ccagcgtttt gtccctggct tgccggttag gcaccgaggc gaaaagcttg
    13261 cgcgaatagg gatgcgaggg attgccgaag aacgcctcgc gcggcgccat ctcgacgatt
    13321 tcgccggcgt acatcacagc cacgtggtgc gccatttccg ccaccacgcc gagatcgtgg
    13381 gtaatcagca ggatcgacat gcccatgcgc ttctgcagac tgcgcagcaa gtccagaacc
    13441 tgcgcctgta tggtgacgtc cagcgccgtg gtcggttcat ccgcgatcag caggctgggc
    13501 tcgcaggcca gcgctatcgc aatcatgacc cgctgcttca tgccgccgga aagctggaac
    13561 gggtactcgt ggacacgtcc ggccgggtcc ggaatgccga ctgcattcag cagttcgatc
    13621 acccgggcgt tcacctcggc cccgcgcaat gccgtatggc gctccagcac ctcggcaatc
    13681 tgcgcgcccg cggtcatcac cgggttcagg ctcgcgccgg gttcctggaa aatcatgccg
    13741 atcctgcgcc cgcgtacccg ccgcatcgcg gcttccggga gctgcagcag ttcctcaccc
    13801 tccagccgca ctgcgcccga cacgatgcgg ccggcgtccg gcagcagccg cgtgatcgac
    13861 aacgccgtca tcgacttgcc gctgcccgat tcacccagca gggcgaaggt ctcgccgcgt
    13921 tcgatcgaga aggaaagccc gtcgacggcg cgcaccatcc cttcgttggc ggccagccag
    13981 gttgacaggt cctcgacctc gagcagggta tcggccatga caggctaact gtttttcacc
    14041 gcgggaagat acttggaaat gaatgccggg gtcgccagcc ttccccggat gcgcggatcg
    14101 aaggcatcgc gcacggcgtc ggcgaacagg ttggcggata gcacaagcat cagcatgaac
    14161 acgaatgccg cggccagcga ccaccacacc atgggttcgc gcgccatctc caggcgcgcg
    14221 gaattgatca tggtgccgaa gctgatcatg gtcgggtcca cgcctacgcc gacgtaggac
    14281 agcaccgctt ccgccagcac caggccgctg aaatccatga ccaccgcgat cagcacgata
    14341 tgcatcacgt tgggcaggat gtggcgggtg atgatgcgga aaccggaaac cccgaaagcc
    14401 tgtgccgcct ggatgtattc catctcgcgc agcttcaacg actccccgcg caacaaccgg
    14461 cacaatccgg tccagctggt cacgccgaga atcaggcaaa ggaccagcaa cttggcgtcg
    14521 gctcgctgct gcacggtctc gaacagttcg gcatgcttgt ccatgagaac ctgcagcatc
    14581 agcaccgagg ccgcgatgag caggatgccg ggaatggaat taagcgtggt gtagacgtac
    14641 tggataacgt cgtccaccca gccgcgcagg taaccggcca tgatgccgag caacagcgcg
    14701 aacggcagca tcaccatcgt ggtgagggta ccgatcacaa gcccggtgcg gatgctcttg
    14761 agcgcctggt agaaaacgtc ctgccccacc ttgtcggtgc ccagaacgtg atatttcacc
    14821 gacagcaaca acatcggcgc cagcaccacc aacagcacgc atgctgtgat gagcatctcc
    14881 cgccacggca ccgcgctttc cccgttccag atctgccgcc aggcgtccga catgcctcgg
    14941 ttgtcgcgcc gcgataacca gatggtcgcg atcagcacca gcagattcca aaccaatatc
    15001 gcagccgcca cgccggcaat cgcgcgtttc gccacgtccc atgcccaatc ggtttccgga
    15061 tcgtcgagat gggcgccgcc gtaagtcagc cgggggaaaa gccgcgcctg cctgccatcc
    15121 ggcagttcca ccgtctcctt ggcgaacaaa tgggtcgcca gcggcgcgga gtaagtcttt
    15181 tccggcctgg tgcgcaggtg tgccgccgcc acgtcgaaca gcgacagcat ctcgacagcg
    15241 taactggcct tgccgccagc ggcgccatcc gccaagcgcg ggcgaaaatg cagcgaatcc
    15301 agcagtccga ctgcaacgaa tacggccagg accaccgcgg ccaccatccc gctgcggctt
    15361 tgcgccactt ttctccacgg cgccagcagg tgctcgtgac ggcgcacatg ccaggcgaaa
    15421 accaggatca ccgcgaccag caggtagacc agcgcatcgg tccacagcac caccggcatg
    15481 atgggcagga gactcgtcat tgcagcctga ccctgggatc ggcaatcgtg taggaaaaat
    15541 cagtgaggat cagtccgacg atgtaaagca ccgacccgag aaacaccatg gcccgcacta
    15601 ccgagaagtc ctggttgttg atcgcgtcta tggtgtagct gcccagcccg ggaatggcga
    15661 agaaagattc ggtgatcagg ctgccgagga acaacagcgg gatcgctacc accgcgccgg
    15721 tgaggatcgg gatcatggcg ttgtgcaaca catgcttgaa cagcacgcgc agttccgaca
    15781 gccccttggc gcgggcggtg cgcacgtagt cgcgattgat ctcttcgaga aaaatggtgc
    15841 ggtaccagcg cgcgctcgac ccgacgccgg cgatgatacc gatgatcacc ggcagcacca
    15901 ggaacttcca tgcgctcagg ccttcgccgt atccggaaat cggcaccagg ttccacagct
    15961 tgctgatcag atactgcccg ccgatgatgt agaacagccc cgagaccgac atggcgacca
    16021 cgcacagcac cacgccggca aagtcgatgt aaccgccgcg aaagaacgcc atcagcagcg
    16081 caaccgtgat ataggtcagc atgccgacca gaaaaaccgg cagcgcgatg gcgaaactgg
    16141 gccacatgcg gctgcggatt tcgtggccga tgtcacgacc gtcggcctcg gcgcgcccga
    16201 aatcgaaggc gaacatcttc accgacttgg tgaagaaaat ggtgtcggtg agcttgccgg
    16261 tgccttgcgc agcggaattg acgaacagcg ggcggtcata gccgcgctcc tccttccatt
    16321 tgcgcacggc ttcctcggtc acgcgcttct gcccgagctg catgcgcgcc atgtcgtccg
    16381 gcgtgttgac gaagaagaac agcgcaaagg tgatcacgtt gaccccgacc aggatgggga
    16441 tggcgtagag aacgcggcgg atgaggtagt tgatcatgga cctacaccgc ggccaatttg
    16501 cccccggcac gttcgcgccg gcggtatgag agtaccgcgg ggatcaacgc aagcaccaat
    16561 gcggccagtg cggcaagcac gggccagagc accggcgagt tccactgcgc gcgcatggtt
    16621 tcgcgcagtt gcgagtcgat gcgttgatac ttgagactat tgttggccat cttgttgggc
    16681 ttgcggttgt gcacccaggc gtgcgcgaga gtgaaatcct gcggatgcag cccccaagcc
    16741 cagggggcgt cgaaacgcag catctcgatc atgcggtcga tcagtgcctg ccgctcgggg
    16801 ccgttgtcca ttgcgcgcac gcgttcgaac aggcggtcgt actccgcgtt catgtagttg
    16861 gaggagttct cgccgttctt gagcgcgcgg ctctgcggcc catggaacat gaacagaaaa
    16921 ttctcggggt ccggatagtc cgcgttccag ccgaagaaga acaattgcgc cgtgcccttg
    16981 cgcacctttt cctgaaaacg gttgtaatcg gtattgcgca ccaccaattg cagattgatc
    17041 ttggctaact gcttcaccag ccagtcggcg cgcgacttgc cctccgcacc gaccagggtg
    17101 ccgtcgaagt agatcaccag gggcttgccg gtcgaggctt cgattccgtt cgcataaccg
    17161 gcctcggcca gcagctgctg tgcgtacgcg atcggcttgc gcgtcggcct gccctccacc
    17221 cagtcgtaga cgtagggatt cattccctcg cgcccgtcgc ggtagccgaa tatgcccggc
    17281 gggatcggtc cttgggccgc gacgccgcgc ccgttgcgga agatcgagat catttcctcg
    17341 aagtccagcg cgatggaaat ggcgtggcgc agcttgcgcg cgcgctccga gttgcccccg
    17401 accaccgggt cgagcatgtt gaaggccatg tacagggtgg aggtcgcgac cgaggtctgc
    17461 aacgcgattc ggcgcagttg aatgtcctcg gtcagcgcta tatcgccggc accggtgatc
    17521 tgcaccacct ggtcgaaggt atccgatgag attccggaat aatcgtaata gccctgcagg
    17581 aacttgttcc agtagggaat gctttccttt tccaggctga atatgacctt gtcgatgaac
    17641 ggcatgagtt tgccggcatc gtcgaggagc ccggcttcgg catcgcccgc ttcgccatcc
    17701 gcgggatacg gttctccggc gaaattcgga ttgcgctcca tcaccatcaa ccggttcgga
    17761 ttgttgaccg tgagcatgta cgggccggtg cccaccgggt accagtcgag ggtgaggttt
    17821 ttgccttcgg tcatccccgg ttgcgagtag aagcgatgag cctcttccgg gatcggcgca
    17881 aagaacggca tggccagcca gtaaacgaac tgcgggtact tgccgcggat cttgatgcgg
    17941 tacgtgtagc ggtccagggt ccgcgcgccc tcgagaggga acgtcgtgag gtcgagatag
    18001 gcgtccgcgg gcaggtgctt tgccgcttcc tgcagttgct gtgcgaattc cttcagtccg
    18061 acgatgtact cgctcatcag accgaaaatc ggcgaatgca gcccggggtg cgcaagccgg
    18121 cggatctgaa acacgtagtc ctcggcaacc agttcgcgcg tgccgctgta cccgaagtcg
    18181 cgcagctcga atatgttcct cagatcctcg cgcccgagat tcaaatagcg cggcttgccg
    18241 gactcgccca cggcaaaggc cgggtgcggc tggtacatca ctccctgcct gatgcggatg
    18301 tcgtaaacgc tgaatgccac ccgttcggcc ggcgcggaat cgggcagccg gcggccgtcg
    18361 gcatcgaggt aaacggcgcg cggaatctcc gccgcgccga aaggaaccag ttcgtaaggc
    18421 cgcctgagat agtggtattg caacggcggc atgtagatct gggcgatgaa gacgtactcg
    18481 ttctcggcgt aggactgcac cgggtcgagg tgcttgggcc gctccacgaa ggccgaatac
    18541 aggatgttct ggccgcgctc gcggacggga taggggttat tccatggtcc accgcagccg
    18601 cccaggaaac cgagcagcgc gagcgccacg gctgttgcac tcagccggct catactctgg
    18661 cgcaagtgta gcggaatacc gggtattcaa cccgcgcaca tccgcaactc tcgtagatca
    18721 cggccgtcgg gacgggtcgt ttcgatataa taatgctgga tttccgcgaa acgaggaaga
    18781 aacagcaagg aaacgtcatg ggatttctcg acaaaaagaa aatcctcatc accggcctgc
    18841 tcagcaaccg ttcgatcgcc tacggaatcg ccaaggcaat gcaccgcgag ggcgcgctac
    18901 ttgcattcag cttccagggg gagcgggtca gggatcgcgt ggtcgaactg gcagccgaat
    18961 tcggcagccg gctggtgctg ccctgcgatg tcgccagtga cgccgagatc gacgcgctgt
    19021 tcgccgaact ggcccggcat tgggatggcc tcgacggcat catccactcg atcgcatatg
    19081 cgccccgcga agcactggcc ggcgattacc tggattcggt gacccgcgag gcgtttcgca
    19141 tcgcccacga gatcagttcc tacagctttg ccgcactcgc caaggccggc ctgccgatga
    19201 tgcagggccg caaggccgcg ttgcttacgc tcacctacct cggcgccgag cgttctttcc
    19261 ccagctacaa cgtcatgggg ctggccaagg ccagcctcga agccaatgtc cgctacatgg
    19321 cacaaagcct cgggcccaag ggcatccggg tcaatgcgat ttccgcgggg ccgatcaaga
    19381 cgctcgccgc tgccggcatc aacagcttcg gcaagctgct ggcctacgcc gaaaagcagt
    19441 cgccgctgcg ccgcaacgtt agcaccgagg aagttggcaa tgtcgccgca ttcctgtgca
    19501 gcgacctggc cagcggcatc accggcgaaa tcacctatgt agacgccgga ttcaatacca
    19561 ccggggccgg ccaaatcgaa tagacggaac tccgaaacag gaaagaagcg ctcaccacgg
    19621 aggacgcgga ggacacggag gagaaacaaa aacgaggaag aacatgcgga agttagaggc
    19681 agcactgatg tcgaagtggc caccgatagc gggtttcgaa tctcgaaggt ttttttgcgc
    19741 tcctgctttc ctccgtgtcc tccgcgtcct ccgtggtgag cgcttctttc aataattccg
    19801 atctactgtc cctgctccaa ccgcttcctg tcgaccttga cgtcggcgcg ttcccgcagg
    19861 ctggcgagat aggcgacata ctgttcctgg ccgaccatct ccgcgagttg cttgcccagc
    19921 gccttgcgtt gttccgtatc aacggcggtc acgtccttga ccttggtgac gcggtatacg
    19981 acgaatcgtc cctgcgcagc cggcacgccg gcatagaccg ggagtttgct cacgtcggcg
    20041 ccgaataccg cctgtgcgcc ctcggggtga agaccctggc gcttctgcag cgataccgac
    20101 tgcgacggcg accaggacag cgccgccggc tgaccctgct gcagcttctc cagcgccgcg
    20161 cggccttcct tgccggccag ctcgatcgcc cccttgtcgg tgagatattg catgacatcg
    20221 ctgcgcacct gctccagcgg cagagccgct gccggcttgt gctcgatcac ccgcgctgcc
    20281 agcagcatat tgggttgcac ctcgatcgcc gaggtattgt gtttgttctt cagcacttcg
    20341 tcggagaaca ccgcgtggag aagcttctcg ttgttgaaca gcgggttcgg attgccgccg
    20401 gtccggctga cccagtcgga cttctgcacc gtgagcttga gcgcctccgc cgcgggctgc
    20461 aggctgtcga actgctcgta caccaggttg ttgaaactgt cggcagcttc ggcataggcc
    20521 ttgccgagct tcggcttgcg aacttcgtcg acgatctgtt gtttcaccgc gtcaaaaggc
    20581 gtggtctgcg acgcctttat cgcgtccagt cggatgatgt gatagccgta ctgggtttcg
    20641 accgggcctg ccagctcgcc gattttgagc gcgaaaacgg cggcgtcgaa cggctttacc
    20701 atcagcccgc gagcgaagaa accgagatcg ccacctttct gcgccgaccc ggggtccttc
    20761 gagaatttgg ttgccagttc ggcaaacttg cccggtgcag accggatttg tttcaacagg
    20821 tcctcggcca gcgtcattgc cttggccttg tcctcggcgc tggcacctgc ggatgccgga
    20881 atcagaatat ggctggcgcg ccgttgttcg ggggtccggt aacgttcgat gttcgcctgg
    20941 tagtaattct tcaattcctc gtcggtgacc tttgccgcat gttccacggc atccggggac
    21001 aacaccaggt actccaggcg cacgcgttcc gggatctgga actcgttctt gtgctcttcg
    21061 taatacttc
//
LOCUS       DASQOP010000075        19126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_431161, whole genome shotgun sequence.
ACCESSION   DASQOP010000075 DASQOP010000000
VERSION     DASQOP010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 19126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19126
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431161"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1036)
                     /locus_tag="VE030_02880"
     CDS             complement(158..1036)
                     /locus_tag="VE030_02880"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003272254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZF22391.1"
                     /translation="MKVGFIGLGSMGVPMADNLLKGGHELFVWARRAEAARPLTGKGA
                     KVCASPKEVGASTEVIFTIVTTGTDVEQVVLGKDGVIHGARAGSVLIDCSTIPPRTAR
                     EVAAELARRGVDMLDAPVSGGEAGAQAGTLSIMIGGRAEVFERMKPLLSLLGTTLVHV
                     GESGAGQVAKAANQLVLVVSIQGIAEAMIFASANGVDFKPVWEALTKGFAGSRMLEVF
                     GPRMMQRQFVAGLDARLHHKDANIVLQCAYESKTPVPGAGLAAQSFNALMARAGTRWD
                     SAAILKVIEDMNGRAG"
     gene            complement(1285..2163)
                     /locus_tag="VE030_02885"
     CDS             complement(1285..2163)
                     /locus_tag="VE030_02885"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0019580 - galactarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HZF22392.1"
                     /translation="MKVTFCGLGIMGRPQALNVMKAGHAMAVWARRKESMQPLADAGA
                     TACASPREAAALAEIVFVMVSDTPDVEQVIFGPKGIIEGARSGAVVVDMSTISPVATR
                     DMAKRLADKGIEMLDAPVSGGEAGAINGTLSIMVGGKPEVFARVKPLFECMGRNIVHI
                     GANGAGQVAKACNQIVAAVTLEAVSEGLTLARRNGVDPAKVRDALMGGFAYSKVLEVH
                     GRRILERDFKPGFKAKLHRKDLKIVTDTAAHLGLTLPQASLIASHLNALVGLGCGDED
                     SAAVVKVIERAAGELR"
     gene            complement(2401..3717)
                     /locus_tag="VE030_02890"
     CDS             complement(2401..3717)
                     /locus_tag="VE030_02890"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZF22393.1"
                     /translation="MTDLYELSLREAAQRIRSGRLTSASYMKSLLARVVALEKNVQAW
                     QWLSLDRAMTLAEQADRADGPMRVAHPLHGIPVAVKDNFYTAGIPTEMGCRAYAGYVP
                     DETADVVKRLEAAGAIMLGKTVTTEAAFMVPAKTRNPWNNAHTPGGSSSGSAAAVAAG
                     FVAGALGTQTNGSVIRPAAFCGIVGYKPSSGQMSARGVMPFSPTLDQPGVFARNVADA
                     AFLASCLTVHRTAITPEVKPLRTVPRMAAVRSPVWHLALPEQRAQFEADIARLREAGA
                     SVDVLEIPGEFDGAHKVHRAIMLFEAARAAKKVRAAYRDKFSDYLNTALDEGESMREP
                     DYRDALKARAQLQQSLAQFFDRGYAAIITPPAAGEAPATLTNTGDPRFCTIWTLVGAP
                     AITIPTGKAPNGMPLGLQVVGAAEEENYLLATAAWCERHSPFHGLI"
     gene            complement(3907..4893)
                     /locus_tag="VE030_02895"
     CDS             complement(3907..4893)
                     /locus_tag="VE030_02895"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HZF22394.1"
                     /translation="MKPKVLVTREVFDETIEFLAQHFDVTSNQAKVDFSREAIIERLQ
                     GMNAAVITTSDRIDGEFLSRCPGLKAVCSVSVGFNHIDLDACTRHGVIATNTPGVLTD
                     SVADMALCLTLATVRRLSEGERMVRAKQWKGSHLRQLLGHDLHHATVGVCGFGRIGQA
                     IAKRLRGFDTNLLYTDISRADRKIEDVLQTTYASKEDLLRQSDIVILILPYTPETHHY
                     IGKAELAKMKASAVLINMARGGIVDDAALADALKAGRIWSAGLDVYENEPVVNPALFE
                     LTNIVLTPHIASATEPTRRAMAMLAAQNCVAALTGGAPPNLLNPGYRKYSKA"
     gene            complement(4932..5072)
                     /locus_tag="VE030_02900"
     CDS             complement(4932..5072)
                     /locus_tag="VE030_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22395.1"
                     /translation="MPYLLDVTLGQIFHGMMPFTAIQIVATVLLYAFLQIGMWLPRVL
                     YQ"
     gene            complement(5051..6349)
                     /locus_tag="VE030_02905"
     CDS             complement(5051..6349)
                     /locus_tag="VE030_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HZF22396.1"
                     /translation="MSIPDIPLDFVLFALTLVGVALFHRHTLKVALTGLVMVTLHKLL
                     FTGFKTGPGIAGLVTHLGHEWVILGNLLGLLVGFALLAQHFHDSRAPELLPDFLPDDW
                     KGGFCLLLIVFVLSSFLDNIAAAVIGGTMASAVFRGKVHLGYLAAIVAASNAGGSGSV
                     VGDTTTTMMWIAGVSPIEVLHAYVAAVVAVIFFGVAAARQQQRYAPIQREAPAGIRMD
                     WIRIGIVLFILLAAIVTNVAVNVGFPLLADRFPFIGASVWAALLCAAPLRRPAWSLVP
                     GALKGSVFLLSLVLTASMMPVEKLPAASWQTAFGLGFVSAVFDNIPLTALALKQGGYD
                     WGMLAYTVGFGGSMIWFGSSAGVALSNSFPEARSVGAWLRHGWHVAVAYVLGFFVLLA
                     VVGWQPGATRDAPVPTPPRNHASTPNGAGQQGLDCRTSLT"
     gene            complement(6359..6574)
                     /locus_tag="VE030_02910"
     CDS             complement(6359..6574)
                     /locus_tag="VE030_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22397.1"
                     /translation="MRMRRALQFAAFGLLISAALELLAPAAVMAAGDTATHSAGLFTW
                     IAVILTTLITPPILTLLIRRTAGRSAG"
     gene            complement(6704..7840)
                     /locus_tag="VE030_02915"
     CDS             complement(6704..7840)
                     /locus_tag="VE030_02915"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013433878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HZF22398.1"
                     /translation="MTLEFSRSEPLTMGIELELMILSTHDWDLMRGAGDLLALIDKNP
                     HPGDIKPEITESMIEISTAVHGDYREMLVELSAMRDAVIKSAQRLNLAIAGGGAHPFQ
                     HWSEQRIFPKERFYHLHEMYGYLAKQFTVFGQHIHIGVPSGDDAIYLAHMMSRYIPHF
                     IALSASSPFSQGMDTDFDSSRLNNINAFPLSGTMPFVRDWDEFNAYFDTMRTLQVVET
                     MKDFYWDIRPKPEFGTIEIRVCDTPLTVEKAAVLGAYAQALARHLMLDRPREPSQDVY
                     RVYSFNRFTACRYGMQAGFIDAYARSRRLLKEDIAETLDILAPHALALGSGAALAEIG
                     ASLDSGQNDSGWLRDMFKQTKSLSDVVRLQSRLWAGEQRSQPDR"
     gene            complement(7837..9054)
                     /locus_tag="VE030_02920"
     CDS             complement(7837..9054)
                     /locus_tag="VE030_02920"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZF22399.1"
                     /translation="MDEVSFLPTFPLFAANMMLVGAVLLCGLAAGHVFARYLRLPRIT
                     GFVAAGLVLGPGMLNLLDRSTLAELSIFVDFSLGLILFELGRRLDLRWLMRDGWLLAS
                     GVAESLLSFVFVYWTLRFFDVAPMYAATAAAIAVATSPAVLLMIVHDQRAKGPLTERM
                     LTLTGLNNVIAFLVVTLLFGFVHLEYKANWVRALLHPLYLIAGSAALGYLAFVVARWL
                     ARWFGKREDIQFIMLLGLIVATVGLAISYKFSVLLALLAFGVFAKNLDRNHDLMAVEF
                     GPGGQLFLVVLFVITGAGLQLSELAVAGGLGLAFVLARLAGKLAGLAMFAPLSGLTQR
                     KSALLGVGMVPMSAIAVVMVQGASQLYPEFGAKLSSIVLSAVVILELVGPLATQWALR
                     LAGEIDPAAQERP"
     gene            complement(9274..9921)
                     /locus_tag="VE030_02925"
     CDS             complement(9274..9921)
                     /locus_tag="VE030_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HZF22400.1"
                     /translation="MSARENILQRIRAANGRTGALTPAERETALARLRAHPRGPLPDM
                     GWELLPRFKERCIAMMSSVAEINSVADVPQAVAKYLTENKLPLSGACWPEFANLDWAG
                     AGLQIAARPANGDDKVGITGTYCAVAENGTLMLLSGQDSHATTSLLPDNHIAVVPASR
                     IVRAMEDGWDLLRREHDSLPRQVNFVSGPSRTADIEMTLVMGAHGPFRVHVVIVV"
     gene            complement(9933..11342)
                     /locus_tag="VE030_02930"
     CDS             complement(9933..11342)
                     /locus_tag="VE030_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HZF22401.1"
                     /translation="MQVTSQQFKQAASQKLADPKVQKALKNAKGKLVVARAKSILELD
                     NFEQIREAAARIRDFGLLNLEALLEEWEKNAKTAGAIVHWAETPQDVNRLVIEITQKH
                     KVRKIIKSKSMLGEETDLNHALEAAGLDVRETDLGEYIIQQFGETPSHIIAPAVHKSM
                     DDIADLFTEKHKRPRLTDIAALTHEAREILRPHFLDADMGISGANFLVAETGSTMIVT
                     NEGNGRMTTTMPRVHVAIAGIEKIIATLEDVSLMLRLLTRSATGQSISNYLSFTTGPK
                     RPGDKDGPGEFHVIIVDAGRTRILGSEIREALRCIRCGACMNHCPVYQNIGGHAYGWV
                     YPGPIGSILTPAYVGIENALDLPNAATLCNQCGVVCPVKIPLPELMRKLREKQVERNL
                     RPWTERLGLKLWAWAATRPAVYALMTRIAARVGRMMGGESGMLHKLPAAGREWTRGRD
                     LPAPKGRTFRELRARQKGR"
     gene            complement(11459..12196)
                     /locus_tag="VE030_02935"
     CDS             complement(11459..12196)
                     /locus_tag="VE030_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011549455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HZF22402.1"
                     /translation="MRVGLFVTCLVDLMRPNIGFAALRLLEAAGCEVAVPAAQTCCGQ
                     PGYNSGDRKAAQALARKLIREFSDCDYVVVPSGSCGGMIRAHYPELLSEFPELGEEVE
                     AFARKTYELTDFLVNVARLDRVPGKFGGTVTYHDSCSGLREMNVKSQPRALLSKVPGL
                     TIREMQQPEQCCGFGGTFAVKYGDISTRIADLKCEDVQSSGADALVLGDLGCMLNIEG
                     RLRRRGDTRTLVLHVAEVLAGMDKDEG"
     gene            12570..13349
                     /locus_tag="VE030_02940"
     CDS             12570..13349
                     /locus_tag="VE030_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZF22403.1"
                     /translation="MSEGNGKTSIQVIERMMDLLDALARNNEPVNLKRLANSTGLHPS
                     TAHRILNMMVEFRMVDRVEPGTYRLGIKLLELGNLVKSRINLRQEALPFMQRLHQDLH
                     ETVNLSVRQGDEMVYVERLLSDRTAMRVVHLIGARAPLHITAVGKVFLLDDGPDKCRE
                     YAQRSGLAVYTKNTIRDPGTLTRELERVRKLGYAFDNEEAEKGVSCIGSGIYDDEGRL
                     VAGLSLSAPSNRMDKAWGPRVKEVAEEISRALGYRAGAKAA"
     gene            complement(13351..14235)
                     /gene="pbpG"
                     /locus_tag="VE030_02945"
     CDS             complement(13351..14235)
                     /gene="pbpG"
                     /locus_tag="VE030_02945"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF008668.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HZF22404.1"
                     /translation="MKAFISTLFVCLAAASLPAWAIGELHKTAATHSHFSARPARLPL
                     RSGAVLVLDQETGELLLAKNTDQVLPIASITKLMTAMVVLDSGLPLLESISIEKADVD
                     RMKGSRSKLSVGTTLMRAEMLKLALMASENRAAATLARNYPGGTPAFVAAMNQKSVEL
                     GMSNSRFVDATGLHPSNVSTAEDLAKLADAAHRYPLIREYTTTDYFRIESEGQRRRHV
                     LAFVNSNRLLKSRQWEIGLSKTGYISEAGRCLVMQAMIGDKPRIIVLLDSWGKLSRIG
                     DANRIKRWIEADVMRIRG"
     gene            complement(14444..16273)
                     /locus_tag="VE030_02950"
     CDS             complement(14444..16273)
                     /locus_tag="VE030_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HZF22405.1"
                     /translation="MRDHASRPSGTPSSSRNDWHTIGKLLPYLLEYKGRVAAALAFLA
                     AAKIANVGVPLVMKEIVDSLDVRQAVLALPFALLVIYGVLRLSNTLFAELRDIVFVRV
                     AKRAIRRVALQVFRHLHALSLRFHLDRQTGGVSRDIERGTRGISTLLSYMLFSIIPVV
                     LEFSLVAAVLLTKFDWRFMAVTMVAVVVYLAFTFTVTEWRMDIRRRANELDSKANTRA
                     IDSLLNYETVKYFNNEEFEARRYDENLQKFESADVKNEASLGLLNIGQSLVIAIAVTL
                     LMILAAQGVARGTLTLGDLVLVNGLLIQLYIPLNFLGMVYREIKQALTDMDKMFRLLS
                     ENREVEDRPDAVHLAAAHPAVRFENVDFGYDPRRQILHQVSFEIPAGHTVAVVGASGS
                     GKSTLARLLFRFYDVGGGRITVDGLDIRDVTQKSLRAAIGIVPQDTVLFNDTIYYNIA
                     YGNPEASREEVIAAAQSAHIHDLIESLPDKYEAMVGERGLKLSGGEKQRVAIARALLK
                     DPRVLIFDEATSALDSKSEKAIQAELGRISASRTTLTIAHRLSTIVDADQVLVMEGGR
                     IVERGSHRDLLARGGVYAHMWALQQEKERVEAAELVSPQVKLA"
     gene            16450..16641
                     /locus_tag="VE030_02955"
     CDS             16450..16641
                     /locus_tag="VE030_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22406.1"
                     /translation="MHRAETVEEGLDLQDLGKRVTLAYKEHIGVPTTCFADTRHFASD
                     VRSIEDPAPLSPAPQPPQQ"
     gene            16638..17597
                     /locus_tag="VE030_02960"
     CDS             16638..17597
                     /locus_tag="VE030_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZF22407.1"
                     /translation="MTNQVPAEQGSLAYAASRTLPPQVRLIVRDWLNANHIILTGRDR
                     TVLVDSGYGRDANITLTKVSAELAGRPLDWLVNTHCHSDHMGGNAAVRRAHGCRLSIP
                     IGEAPLIEAWDEQALWLSYADQRCERFEFDDTIAAGEVLDWGDLRWRAIAAPGHDMGA
                     LMFHCEEERLLITGDALWENGFGVLLPGPDREQRLAATRATLEAISRLDVHTVIPGHG
                     ASFAGVDAALERSLRRLEAFERDELRMARHVLKVMFVFSLLDRQRLPLGRIPEYLATV
                     PLYADFNARYFQLNDAALAELIVGDLERAGAVKKLNGYLIAAI"
     gene            complement(18173..>19126)
                     /gene="clsB"
                     /locus_tag="VE030_02965"
     CDS             complement(18173..>19126)
                     /gene="clsB"
                     /locus_tag="VE030_02965"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF008427.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HZF22408.1"
                     /translation="GSRLFPEDRRRQLLEAGVQLLVFRPEVWTFRLRTTRLRRLHRKL
                     VVVDGRIGFCGGINIIDHRDSRGNTAPRLDYAVSVEGPLIADMHAAVRTLWMRTAWTQ
                     LRRRWLPLAKPRLTVEPCGDQHSAFLVRDNVRHRHDIEQAYLHAIERAHREVVIANAY
                     FLPGRRFRNALCDAARRGVRVTLLLQGRVEYILVHYASRALYRQLLDAGVQIHEYTRS
                     FLHAKVAVVDDSWATVGSSNIDPVSLLLAREANVVVVDRTFVHELHGSLMDAIEAGAK
                     PVQRERWARMGLPMRMATGAAYAVLRVLTGISSYGRAHDFL"
BASE COUNT         3691 a         6079 c         5814 g         3542 t
ORIGIN      
        1 caacgaatct tttcattttt ggtctcctgc aaaaaatgaa acccgttcgc cagtcggccc
       61 gcgcagtgga tcgctgtgcg gcacttcaga tgtcggcatt tgcagatgcc acgcggcaaa
      121 gtattgcatt tccggtccag aaggcaagcc ggccggatca ccccgcgcgc ccgttcatgt
      181 cctcgatcac cttgaggatc gcagccgaat cccagcgcgt gccggcgcgc gccatgagtg
      241 cgttgaagga ctgcgccgca agcccggcgc ccggtaccgg cgtcttcgat tcgtacgcgc
      301 actgaagcac gatgttggcg tccttgtgat gcaggcgcgc atccagaccg gcgacgaact
      361 ggcgctgcat catgcgcggg ccgaacacct cgagcatccg gctgccggca aagcctttgg
      421 tgagcgcttc ccacaccggc ttgaagtcaa cgccgttggc gctggcgaaa atcatggcct
      481 cggcgatgcc ctggatcgaa accaccagca ccagctggtt cgccgccttt gccacctgcc
      541 cggcgccgga ttcaccgaca tgcaccagag tcgtgcccag cagcgacagc agcggtttca
      601 tgcgctcaaa tacctcggct cggccgccga tcatgatcga cagcgtgccc gcctgcgccc
      661 cggcttcgcc gcccgagacc ggggcatcga gcatgtccac gcccctgcgt gccagctccg
      721 ccgccacctc gcgcgcggta cgcggcggga tggtgctgca atcgatcagc accgatccgg
      781 ccctggcacc gtggattacg ccatccttgc ccagcacgac ctgttcgacg tcggtgccgg
      841 tggtgacaat ggtgaaaatc acttcggtac tggcgccgac ttctttcggc gatgcacaca
      901 ccttcgcccc cttgccggtc agcggccgcg cggcctccgc gcggcgcgcc cacacgaaaa
      961 gctcatggcc gcccttgagc aggttgtccg ccatcggcac acccatcgat cccagcccta
     1021 tgaatcccac tttcattttc gtttactcca gctctctctc aagaaaactt ttcaccacgg
     1081 aggacacaga ggacacggag gaaaggcaaa agaaaaaaat gcttgggttg aaacggttct
     1141 ttggcagatc atccatcgca gtaaaggtgg gcagcacagg ttcgcgaaca gtaacaccgc
     1201 ttttaactcc agcttgcttt ccctcgtttt tgtttttcct ccgtgtcctc tgcgtcctcc
     1261 gtggtgagtg ttttctgtat ttcctcagcg aagctcgccg gcggcgcgct cgatgacctt
     1321 caccaccgcc gccgagtctt catcgccgca ccccaggccg accagcgcat tcaggtgtga
     1381 ggcgatcagg ctcgcctgcg gcagcgtgag ccccaggtgg gcggcggtgt cggtcacgat
     1441 cttcaggtct ttcctatgaa gtttcgcctt gaatccgggt ttgaagtcgc gttccaggat
     1501 gcgcctgccg tggacctcca ggactttgct gtaggcaaaa ccgcccatca gcgcatcgcg
     1561 caccttggcc ggatcgacac cgttgcggcg cgccagcgtc agtccctcgg acaccgcttc
     1621 cagcgtcacc gcggctacga tctggttgca ggccttggcc acctgcccgg cgccgttagc
     1681 gccgatgtgc acgatgttcc tgcccatgca ttcgaacagc ggcttgactc tggcaaacac
     1741 ttcgggcttt ccgcccacca tgatcgagag cgtgccgttg atcgcgccgg cttcgccacc
     1801 cgaaaccggc gcatccagca tctcgatgcc tttgtctgca agtctcttcg ccatgtcgcg
     1861 ggtggccacc ggcgagattg tgctcatgtc cactaccacg gcgccggacc tggcgccttc
     1921 gatgatgccc ttcggtccga agatcacctg ctccacgtcc ggggtgtcgg acaccatcac
     1981 gaacacgatc tctgcgagtg ccgcggcttc gcggggactg gcgcacgcgg ttgcgcccgc
     2041 atcggccagc ggctgcatgg actccttgcg tcgcgcccac accgccatcg catggccggc
     2101 cttcatgacg ttcagtgcct gcggccggcc catgatgccc agcccgcaga atgtgacttt
     2161 catctgaatt ttctcccaaa ccctgaaaaa ccctcaccac ggaggacgcg aaggacacgg
     2221 aggaaaggca agagcaaaaa gcattctcgg gttaaaaccg ttcattggct gatcatccgt
     2281 cgcagtgaaa attggctgca caggttcgcg aacagccccc caccttcaac cccagtttgc
     2341 ttttcctcgt ctttgttttt cctccgtgtc ctccgcgtcc tccgtggtta acgcgtcgac
     2401 ctagatcaag ccgtggaaag gcgaatgccg ctcgcaccac gcagcggtgg cgagcagata
     2461 gttctcttcc tcggctgccc cgacgacctg cagacccagc ggcatgccgt tcggagcctt
     2521 gccggtggga atggtgatcg ccggcgcacc aaccaacgtc cagatggtgc agaaacgcgg
     2581 atctccggta ttggtgagcg tggccggtgc ctcccccgcc gccggcgggg tgatgatggc
     2641 tgcgtagccg cggtcgaaaa actgcgccag gctctgctgc aattgcgccc gggccttcag
     2701 cgcatcacgg taatcgggct cgcgcatgct ctcgccctcg tccagtgccg tgttgagata
     2761 gtcggaaaac ttgtcgcgat acgccgcgcg cacctttttc gccgcgcgcg ccgcctcgaa
     2821 cagcatgatg gcgcgatgga ctttgtgcgc gccgtcgaac tcgccgggga tctccagtac
     2881 gtccaccgat gcgccggcct cgcgcagccg cgcgatatcg gcttcgaact gcgctcgttg
     2941 ttcgggcaaa gcaaggtgcc acaccggcga gcgcaccgct gccatgcgcg gcacggttct
     3001 cagcggcttg acctccggcg tgatcgcggt acggtgcacc gtcaggcaag aagccaggaa
     3061 cgcggcatcg gcaacgttgc gcgcgaacac gccgggctga tcgagtgtcg gcgaaaacgg
     3121 catcacgccg cgcgcggaca tctgcccgga gctgggcttg taaccgacga tgccgcagaa
     3181 agccgccgga cggattaccg agccgttagt ctgggtgccg agcgcgccgg ccacgaatcc
     3241 cgccgcaacc gcggcggccg aaccgctgga ggaaccgccg ggggtgtgcg cgttattcca
     3301 cgggttgcgg gtcttggccg gaaccatgaa cgccgcttcg gtggtcaccg tcttgccgag
     3361 catgatggcg ccggccgctt cgaggcgctt caccacgtcg gcggtttcgt ccgggacgta
     3421 accggcgtag gcgcggcagc ccatctcggt gggaattccg gcagtataga agttgtcttt
     3481 caccgcaacc ggaatgccgt gcagcggatg cgccacgcgc atcggaccgt cggcccggtc
     3541 tgcctgttcc gccaacgtca tcgcacgatc gaggctcaac cactgccagg cctggacgtt
     3601 cttctccaaa gccacaaccc gcgccagcaa acttttcatg taactggcgc tggtcagccg
     3661 ccccgagcgg atgcgctgtg cggcttcacg caggctgagt tcgtataggt ctgtcatatg
     3721 ttcgattttt caccgcaaag acgcaaaaaa cgcaaagaaa agcagaaaaa taaactggaa
     3781 aacctgcaat gtgcagaacc cgctttcacc gcgagtatcg gctctcctcg tcgcgaaggt
     3841 tatgaaccgt tcttgctcgt tctccgggtt tccatcgcgt tctttgcgtg tttgcggtga
     3901 ataggtttac gccttggaat atttgcggta gcccggattg agcaggttgg gcggcgcgcc
     3961 gccagtcagc gctgcaacgc agttctgcgc ggcgagcatt gccatggctc gccgagtggg
     4021 ttcggtggcg ctggcgatgt gcggtgtgag cacgatattg gtcagttcga acagcgctgg
     4081 atttaccact ggctcgtttt cgtagacatc caggccagcg gaccagatgc gccctgcttt
     4141 cagcgcatct gccagcgccg cgtcatcgac gattccgcca cgcgccatgt tgatcagcac
     4201 cgccgaggcc ttcatctttg cgagctccgc tttgccgatg tagtggtgcg tctcaggcgt
     4261 gtaaggcaga atcagaatga ctatgtccga ctgccgcagc aaatcctcct tgctggcata
     4321 ggtggtctgg agcacatcct cgatcttccg gtcggcgcgg ctgatgtccg tgtagagcag
     4381 attggtgtcg aacccgcgca ggcgcttggc gatcgcctgg ccgatcctgc cgaaaccgca
     4441 tacccctacc gtggcatggt gcaggtcgtg tcccagcagc tgcctgaggt gcgagccctt
     4501 ccactgtttc gcacgcacca tgcgctcacc ttcgctcagc cggcgcacag tcgcgagtgt
     4561 caggcaaagc gccatgtcgg caacgctgtc ggtgagcacg cccggggtat tggtggcaat
     4621 caccccatgc cgtgtgcagg cgtccaggtc gatgtgattg aagcccacgc tcacgctgca
     4681 caccgctttc aagcccgggc agcgcgacag aaactcaccg tcgattcgat ccgaggtggt
     4741 gataaccgcg gcattcatgc cctggagccg ctcgataatc gcctcccggc tgaagtccac
     4801 cttcgcctgg tttgaggtga cgtcgaagtg ttgggcgagg aattcgatcg tttcatcgaa
     4861 cacctcccgg gtaaccagaa ccttgggttt catcattctc ccctcttgtt ttcacgctgg
     4921 agtcgccaat tttactggta aagcacacgc ggcagccaca ttccgatctg taggaacgcg
     4981 tacagcagca ccgtcgccac gatctggatg gctgtgaacg gcatcatgcc gtggaagatc
     5041 tggcccagcg ttacgtcaag gaggtacggc aatcaagacc ttgctgcccg gcaccattcg
     5101 gcgtcgaagc atggttgcgg ggaggcgtcg gaaccggtgc gtcgcgagtg gctccgggtt
     5161 gccaaccgac cacagccagg agaacaaaga agcccagtac atacgccacc gcgacatgcc
     5221 agccgtgccg cagccacgcg ccgaccgacc tcgcctcggg gaacgagttg gaaagcgcca
     5281 cgcccgccga ggagccaaac cagatcattg acccgccaaa accgaccgtg taggcgagca
     5341 tcccccagtc gtacccgccc tgtttgagag ccagcgcggt aagcggaatg ttgtcgaaca
     5401 ccgccgagac gaaacccagc ccgaaagcag tctgccagga ggcggcgggc agcttctcga
     5461 ccggcatcat cgaggccgtg agtaccagcg acaacaggaa gacactgcct ttcaacgcgc
     5521 ccggcacaag agaccacgcc ggccgccgca acggcgccgc gcacaacagc gccgcccaga
     5581 ccgaggcacc gatgaacggg aagcgatcgg caagcagcgg gaagccgacg ttgactgcga
     5641 cgttggtgac aatagcggca agcagaatga acaggacgat gccgatcctg atccagtcca
     5701 tgcggatgcc ggcgggcgct tcgcgctgga tcggtgcata gcgctgttgc tggcgtgcag
     5761 ccgccacacc gaaaaaaatt accgcgacga ccgccgcgac gtaggcatgg agcacctcga
     5821 tcggactcac cccggctatc cacatcatgg tcgttgtggt gtctccgacc acgctgcctg
     5881 agcccccggc attggacgcc gcgacaatgg cggcgaggta accgaggtgc actttgccgc
     5941 ggaacaccgc cgaggccatg gtgccgccga tcaccgccgc ggcgatgttg tcgagaaagc
     6001 tcgacagcac gaaaacaatc agcaacaggc agaacccgcc cttccagtcg tcaggcagaa
     6061 aatcgggcag cagctctggc gcgcggctgt cgtggaagtg ctgcgcgagg agcgcgaacc
     6121 cgaccagcaa ccccaataaa ttacccagga tcacccactc gtgtccgaga tgcgttacca
     6181 gtcccgcaat acccggcccg gtcttgaagc ctgtgaacag aagcttgtgc aaggtgacca
     6241 tgacgagtcc ggtcaaggca accttcaggg tgtgcctgtg gaatagggcc acgcccacca
     6301 gggtgagcgc gaacaggacg aaatcgagcg gaatatccgg gatcgacacg gggcgaccct
     6361 agcccgcaga acggcctgcc gtccgcctga tcagcagcgt caggatcggc ggggtgatca
     6421 acgtggtcag gatcaccgca atccacgtga acaacccggc cgaatgcgtg gccgtatcgc
     6481 cggctgccat caccgcagcc ggcgcgagca gttccagcgc cgcggaaatc aacaggccaa
     6541 atgccgcaaa ctgcaacgcg cgacgcattc tcacgcggac gttacctccc cggtcttgca
     6601 atgcttcatc gcgatgcccc gattcacgaa gccggaatca taagggcagc ggagcgtcgc
     6661 cagcaaaccg cgacagactg tgcggctcaa tttgcgccgc cgttcagcgg tcgggctgcg
     6721 atcgctgctc tcccgcccat agcctgctct gcaggcgcac gacgtcgctc agggatttag
     6781 tctgcttgaa catgtcgcgc agccagccgc tgtcgttctg accgctgtcg aggctcgcgc
     6841 cgatttccgc gagcgcggct ccagaaccca gcgccagcgc gtgcggcgcc agaatatcga
     6901 gggtctcggc gatgtcctcc ttgagcaagc ggcggctgcg tgcataggcg tcgatgaacc
     6961 cggcctgcat tccgtagcgg caggcggtga agcggttgaa gctgtagacg cggtacacgt
     7021 cctgcgaggg ttcgcgcggc cggtcgagca tcagatggcg cgccagtgcc tgcgcgtagg
     7081 caccgagcac cgcggctttc tcgactgtga gcggggtatc gcagacgcgg atctcgatgg
     7141 tgccgaattc cggtttgggg cggatgtccc agtagaaatc cttcatggtc tccaccacct
     7201 gcagcgtgcg cattgtgtcg aagtaggcgt tgaactcgtc ccagtcgcgc acgaaaggca
     7261 tggttccgga cagcgggaag gcgttgatgt tgttcagccg cgacgaatcg aagtcggtat
     7321 ccattccctg cgagaacggc gaggacgcgg acaacgcgat gaaatgcggg atgtaacgcg
     7381 acatcatgtg cgcgaggtag atggcgtcgt cgcccgacgg cacgccgatg tgaatgtgct
     7441 ggccgaacac ggtgaactgc ttggccaggt atccgtacat ctcgtgcagg tgatagaaac
     7501 gctccttggg gaagatgcgc tgctcgctcc agtgctggaa cggatgggcg ccgccgccgg
     7561 cgatcgcgag attgagccgc tgcgcacttt tgatcacggc gtcgcgcatc gccgaaagct
     7621 cgaccagcat ctcgcggtag tccccgtgca ccgcggtgct gatctcgatc atgctttcgg
     7681 tgatttccgg cttgatatcg cccggatgcg ggtttttgtc gatcagcgcc aacaggtctc
     7741 cggcgccgcg catcaggtcc cagtcgtgag tgctcaggat catgagctcg agctcgattc
     7801 ccatcgtcag cggctcggag cggctgaact ccagcgtcat ggccgctcct gcgcagccgg
     7861 atcgatctcg cccgcgagcc tgagggccca ctgggtcgcg agcggaccga ccagctcgag
     7921 tatcaccacc gcggacagta cgatcgaaga cagtttcgcc ccgaactccg ggtacagctg
     7981 cgaagccccc tgcaccatca ccaccgcgat cgccgacatc ggcaccatgc cgacaccgag
     8041 caacgccgac ttcctctgcg tcaggcccga gagcggcgcg aacatcgcca ggccggccaa
     8101 cttgcccgcc aggcgcgcca gcacgaaggc caagcccagg ccacccgcaa ccgccagttc
     8161 ggaaagctgc agcccggcgc cggtgatgac gaacagcacc accaggaaaa gttggccgcc
     8221 tggaccgaac tccaccgcca tcagatcgtg attgcgatcg aggttcttgg cgaacacgcc
     8281 gaaagcgagc agggccagca acacggagaa cttgtacgaa atggccagtc cgaccgtagc
     8341 cacgatcaat cccaacagca tgatgaactg gatgtcctcg cgcttgccga accagcgtgc
     8401 cagccagcgc gccaccacga aagcgagata accgagcgcg gccgagccgg cgatgagata
     8461 aagcgggtgc agcaaagcac gcacccaatt ggccttgtac tcgagatgga cgaaaccgaa
     8521 cagcagcgtg accaccagga atgcgatgac attgttgagc ccggtgagcg tcaacatcct
     8581 ttcggtgaga ggtcccttgg cgcgctggtc gtgcacgatc atcagcagca ccgcaggcga
     8641 cgtcgccacc gcgatggcag ccgcggtcgc cgcgtacatc ggtgccacgt cgaagaaccg
     8701 caacgtccag taaacgaaca cgaacgacag caggctttcg gcgacgccgc tcgcgagcag
     8761 ccagccatcg cgcatgagcc agcgcaggtc gagccgccgc cccagttcga acaggatcag
     8821 tcccagcgag aaatcgacga agatcgacag ctccgcgagc gtcgatcggt cgagcaggtt
     8881 gagcatgccg ggacccagaa caagtccggc agcgacgaat ccggttatgc gcggcagccg
     8941 caggtaacgc gcgaacacgt gtccggccgc aagcccgcac agcagcaccg cgccgaccag
     9001 catcatattg gcggcgaaca gcgggaaggt gggcaggaag ctcacttcgt ccatgagtcg
     9061 gtctcgggag ggcatcggaa gccggtgcgg gaagcggcga aaataccgcg gtttgacagc
     9121 gcgaaaaagc gctaagtgct gaaagccggg ggaattttag gttccgtgcg gaagccgatc
     9181 aaagcgattt acatttctta acatccggct tccctcttgt taggctttta atgttcttct
     9241 ttgtgatctc tgcggctaaa aggttttttc agcttagacg acaatgacga cgtgtacacg
     9301 gaacggcccg tgggcaccca tcaccagcgt catttcgatg tcggcggtgc gcgaaggccc
     9361 ggacacgaaa ttcacctggc gcggcaggct gtcgtgttcc ctgcgcaaaa gatcccaacc
     9421 gtcttccatg gcgcggacga tgcgcgatgc cggaacgacg gcgatgtggt tgtccggaag
     9481 caggctggtg gtggcatggc tgtcctggcc cgacagcagc atcagcgtgc cgttttccgc
     9541 caccgcacag taagtaccgg tgatgccgac cttgtcgtcg ccgttggcgg gccgcgccgc
     9601 gatctgcaga cccgcgcccg cccagtccag gttcgcgaac tccggccagc acgctccgct
     9661 cagcggcagc ttgttctcgg tcaggtactt cgccactgcc tgcggcacgt ccgccaccga
     9721 gttgatttcg gcaacgctgg acatcatggc gatgcagcgt tccttgaagc gcggcagcaa
     9781 ttcccaaccc atgtcaggaa gcgggccgcg cggatgcgca cgcaaccgcg ccagcgccgt
     9841 ttcccgctct gctggggtca gcgcgccggt ccggccattg gctgcgcgga tgcgctgcag
     9901 gatgttctct ctcgcactca tcgtgttctc cctcagcggc ctttttgccg cgcgcgcaat
     9961 tcgcggaagg tcctgccttt cggtgcgggc aggtcgcggc ctctggtcca ctcccttccc
    10021 gcggccggca gcttgtgcag catcccggac tccccgccca tcatcctgcc cacgcgtgcc
    10081 gcgattctcg tcatgagcgc gtacacggcg ggacgggtcg ccgcccaggc ccacagcttg
    10141 agccccaggc gctcggtcca aggacgcaga ttgcgctcga cctgcttctc gcgcagcttg
    10201 cgcatcagct ccggcagcgg gatcttgacc gggcatacca cgccgcactg gttgcacagc
    10261 gtcgccgcgt tcggcagatc gagcgcgttc tcgatgccga cataggccgg cgtcaggatc
    10321 gagccgatcg ggccgggata gacccagccg taagcgtggc cgccgatgtt ctgatacacc
    10381 gggcaatggt tcatgcaggc gccgcagcgg atgcagcgca gcgcttcgcg gatctcggag
    10441 ccgagtatcc gggtgcgccc ggcgtcgacg atgatgacgt ggaattcgcc gggtccatcc
    10501 ttgtcgccag ggcgcttcgg cccggtggta aacgataggt aattgctgat cgactgtccg
    10561 gtcgcggagc gggtcagcag ccgcagcatc agcgacacgt cctccagcgt ggcgatgatc
    10621 ttctcgattc ccgcgatcgc cacgtgcacc ctgggcatgg tggtggtcat gcgaccgttg
    10681 ccctcattgg tgacgatcat ggtggagccg gtttcggcca ccaggaaatt ggcgccgctg
    10741 atgcccatgt cagcgtcgag aaagtgcggg cgcaggatct cgcgcgcttc gtgggtcaac
    10801 gccgcgatat cggtgagccg cgggcgcttg tgcttctcgg tgaacaggtc ggcgatgtcg
    10861 tccatggact tatgcaccgc cggcgcgatg atgtgcgacg gcgtttcgcc gaactgctgg
    10921 atgatgtatt cgccgaggtc ggtctcgcgc acgtccaggc cggccgcctc caacgcatgg
    10981 ttgagatcgg tttcctcgcc gagcatcgac ttggacttga tgatcttgcg caccttgtgt
    11041 ttctgcgtga tctcgatcac cagccggttg acgtcctgcg gcgtctccgc ccagtggacg
    11101 atcgcaccgg cggtcttggc gtttttctcc cactcctcca gcaacgcttc caggttcagc
    11161 aggccgaaat cgcggatgcg cgccgccgct tcgcgaattt gctcgaagtt gtcgagttcg
    11221 agtatggact ttgcgcgcgc aaccaccagc ttgcctttgg cgttcttgag cgctttctgt
    11281 accttcgggt cggcaagctt ttgactggcg gcttgcttga attgttggga tgtgacttgc
    11341 atggcttgaa tacttgatcg gttagcgacg gtggaacaag tatgaaggct gaaggatgaa
    11401 ggcggaagta tggtgtgcgc catgcgcacg cattgcttta cccttcatcc ctcatctctc
    11461 atccttcatc cttgtccatg cccgccagca cctccgcgac atgcagcacc agggtccggg
    11521 tgtcgccgcg tcgccgcagc cgaccttcga tgttcagcat gcagccgagg tcgcccagta
    11581 ccagggcgtc ggcgccgctc gattgcacgt cttcgcactt gagatcggcg atgcgcgtgg
    11641 agatatcgcc atacttcacc gcgaaagtgc cgccgaatcc gcagcactgc tcgggttgct
    11701 gcatttccct gatggtcaga ccgggcactt tcgacaacaa cgcgcgcggc tgggatttca
    11761 cgttcatttc gcgcaggccg gaacaggagt cgtgataagt gacggtgccg ccgaatttgc
    11821 cgggaacccg atccagcctg gcgacgttca ccaggaaatc ggtcagttcg taggtcttgc
    11881 gcgcgaaggc ctcgacttct tccccgagtt cgggaaattc agacaacagt tcggggtagt
    11941 gcgcacggat catgccgccg caggagccgg aaggcacgac cacataatcg caatccgaga
    12001 attcccgaat cagttttctt gccagggcct gcgctgcctt tcgatcgccg gagttgtaac
    12061 ccggctgccc gcagcaagtc tgcgcggccg gcacggccac ttcgcatccg gctgcttcga
    12121 gcaaacgaag cgccgcaaaa ccgatgttcg gccgcatcag atcaaccaaa caggtgacaa
    12181 atagcccgac tctcattttt tctctaccat ttctttctag atcttcaccg caaagacgca
    12241 gcggcgcaaa ggaaacgcaa aggaaagcaa attcatgtag acggcaaacc taagaagctg
    12301 aggccgcgac caaatgactc tcgaatcgca ttcgctttct ttgcgtgtac ttcttcgcgt
    12361 ctctgcgtct ttgcggtgag gtttagatag atgagctggc gcgtgcagta atacggtcca
    12421 ggcggccaaa cacgggtgtg aggcgcagcg cagcaaggcg cgaatcatag catagggggt
    12481 ttcgggtcct gcgctgccgg gttgttctct gcggcttcgc catgtaaaat tgccgcattg
    12541 cacaatgctt ctgaaaagcg agggaaatcg tgtccgaagg gaacggcaag acctcgatcc
    12601 aggtcatcga gcgcatgatg gatctcctcg atgcgctggc ccgcaacaac gaaccggtga
    12661 atctgaagcg gctggcgaat tccaccgggc tgcatccgtc caccgcgcat cgcatcctca
    12721 acatgatggt cgaattccgc atggtggatc gggtcgagcc cggcacctac cggctgggca
    12781 tcaagcttct ggaactcggc aacctggtca agtcgcgcat caatctgcgc caggaggcgc
    12841 tgcctttcat gcagcgcctg catcaggacc tgcacgaaac ggtcaatctc tcagtgcgcc
    12901 agggcgacga aatggtctac gtcgagcgcc tgctttcgga ccgcaccgcg atgcgggtgg
    12961 tgcatctgat cggggcgcgg gcaccactgc acatcaccgc cgttggcaag gtgttcctgc
    13021 tggacgacgg cccggacaaa tgccgtgagt acgcgcaacg ctctggcctg gcggtgtaca
    13081 ccaagaacac cattcgggat cccggcacac tcactcggga gctggagcga gtgcgcaagc
    13141 tcggctatgc gttcgacaac gaggaggcgg aaaaaggcgt gtcctgcatc ggttccggca
    13201 tctacgacga cgagggccgg ctggtggcgg gcctttcgct gtcggctccg tcgaaccgca
    13261 tggacaaggc ctgggggccg cgggtaaagg aggtcgcgga ggagatttcc cgcgcgcttg
    13321 gataccgcgc cggggccaag gctgcttgac tcaaccccgg atgcgcatga cgtcggcttc
    13381 gatccagcgc ttgatgcggt tggcgtcgcc gatgcgggaa agttttcccc aggagtcgag
    13441 caatacgatg atcctcggct tgtccccgat catcgcctgc atcaccaggc agcgtccggc
    13501 ttcactgatg tagccggtct tggacagacc gatttcccac tggcggcttt tcagcaggcg
    13561 gttggaattg acgaacgcca gcacgtggcg gcggcgttgt ccttcggact cgatgcgaaa
    13621 ataatcggta gtggtgtatt cgcggatcaa cggatagcga tgcgccgcat ccgccagctt
    13681 ggcaaggtct tccgcggtgg aaacgttgct ggggtgcagg ccggtggcgt cgacgaagcg
    13741 tgaattggac attccgagct cgacgctctt ctggttcatt gcggcaacga acgccggtgt
    13801 gccgccgggg taattgcgcg ccagtgtcgc tgccgcacgg ttttcggacg ccatgagcgc
    13861 gagcttcagc atttccgcgc gcatcagcgt ggtgcccacg gacagcttcg agcgcgaccc
    13921 tttcatgcgg tccacgtcgg ccttttcgat gctgatggat tccagcagag gcaatcccga
    13981 gtcaaggacg accatcgcgg tcataagctt ggtgatcgaa gcgatcggca atacctggtc
    14041 ggtgttcttg gccagcaaca gctcgccggt ctcctggtcg agcaccaata cggcgcccga
    14101 gcgcagcggc aggcgggccg gcctggccga aaaatgcgag tgtgtagccg ccgttttatg
    14161 cagttcgccg atcgcccaag cgggcaggct cgcggcggcg agacaaacaa acagcgtgct
    14221 gatgaatgct ttcatctgtg gataacgcga cagagcaccg ttaaagtatt gttttaaaac
    14281 ttcaatcctg atcgccacaa cacccgaaac gccgaatggg cgaagatcac ggcaaagctt
    14341 ccctgaccac cctgctttac caagcaataa cataacaaaa atcaatgact aagacaagac
    14401 tctcaactaa ttttttaacg tcggttttgc ggactagctg gcatcaggca agtttgacct
    14461 gcggcgaaac caactccgca gcctcgaccc tttccttctc ctgctgcagg gcccacatgt
    14521 gggcgtacac gccgcctctg gcgagcaaat cacggtggct gccgcgctcg acgatgcgcc
    14581 cgccttccat gaccaggacc tgatcggcat cgacgatcgt ggacaaccgg tgcgctatgg
    14641 tgagtgtggt gcggctggcg gatatgcgcc caagctccgc ctgaatcgcc ttctcggatt
    14701 tggaatcgag cgcggaggtt gcctcgtcga aaatcaggac ccttgggtcc ttcagcagcg
    14761 cccgcgcgat cgccacccgc tgcttttcgc ctccggagag cttcaagccg cgctcgccca
    14821 ccatcgcctc gtacttgtcg ggcaagctct cgatcagatc gtggatatgc gcagactggg
    14881 ccgccgcgat cacctcctcc cggctggcct cgggattgcc gtaggcgatg ttgtaataga
    14941 tcgtgtcgtt gaacagcacc gtgtcctgcg gcacgatgcc gatcgccgcg cgcaggcttt
    15001 tctgcgtgac gtcgcgaata tcgagaccat cgacggtgat gcgcccgccg cctacgtcgt
    15061 agaagcggaa cagcagccgc gccagcgtgg acttgcccga gccgctcgcg cccaccaccg
    15121 cgacggtgtg cccggcggga atctcgaaac tcacctgatg cagaatctgg cgccgcggat
    15181 cgtagccgaa gtccacgttc tcgaagcgca ccgccggatg cgccgccgcc aggtgcaccg
    15241 cgtccggccg gtcttcgacc tcgcgattct cggaaagcag ccggaacatc ttgtccatgt
    15301 cggtgagcgc ctgcttgatt tcccggtaca ccatgccgag gaaattgagt ggaatgtaga
    15361 gctggatcag caggccgttg accagcacca gatcgcccag cgtcagcgta cccctggcca
    15421 ccccctgggc tgccagaatc atcaaaagcg tcaccgcgat cgcgatgacc aggctctggc
    15481 cgatgttgag caggccgagc gaggcctcgt tcttgacatc ggccgactcg aacttctgca
    15541 gattctcgtc gtagcgccgc gcctcgaact cctcgttgtt gaaatacttc accgtttcgt
    15601 agttcaacag gctgtcgatc gcgcgggtgt tggccttcga atcgagctcg ttggcgcggc
    15661 ggcggatgtc catgcgccac tcggtcacgg tgaaggtgaa cgccaggtag accaccactg
    15721 cgaccatcgt caccgccatg aagcgccagt cgaacttggt cagcagcacc gccgccacca
    15781 ggctgaactc cagcaccacc gggatgattg agaaaagcat atagctcaac agcgtggaaa
    15841 tgccgcgcgt cccgcgctcg atgtcgcgcg acactccacc ggtctggcga tccaggtgaa
    15901 accgcagcga cagtgcgtgc agatggcgga acacctgcag cgcgacccgc cggatcgcgc
    15961 gcttggccac gcgcacgaac acgatgtcgc gcagctcggc aaacagcgta ttggaaagcc
    16021 gcagcacgcc gtagatcacc agcagcgcga acggtagcgc cagcaccgcc tgcctgacgt
    16081 ccagggaatc gacgatttcc ttcatcacca gcggcacgcc gacgttggcg atcttcgcgg
    16141 cggcgaggaa ggcaagcgca gccgcgaccc ttcccttgta ttccagcagg tagggcagca
    16201 acttgccgat ggtgtgccag tcgttacggc tcgacgaggg cgtgccggat ggccgggatg
    16261 cgtggtctct caagaggacg ggcgattgtg catgatgtac tgcaattcta atccttgatg
    16321 cgattgcgct cgaagctaaa cgcgtcctga tcccatgcga tgacaaaggg aaagaccagg
    16381 ccggaaagac tgctggaacg gacgcaccaa acatgtcgcc gcggcccgac actccggtga
    16441 tgtatattga tgcatcgtgc agaaactgtc gaagaagggc tggatctgca agacttggga
    16501 aagcgcgtaa cgcttgccta caaggagcac atcggcgtgc ccaccacctg ctttgcggac
    16561 acgcgtcact tcgccagcga cgtgagaagt atcgaggatc cggcgccgct ttcgccggcg
    16621 ccgcaaccac cgcaacagtg acaaaccaag tccccgcgga acaaggctcc cttgcgtacg
    16681 ctgcatcccg cacgctgccg cctcaggtgc ggttgatcgt gcgcgactgg ctgaacgcca
    16741 accacatcat cctcaccggc cgcgaccgca ccgtactggt ggatagcggc tacggcaggg
    16801 acgcgaacat cacgcttacg aaggtttccg cggaactcgc cggcaggccg ctggactggc
    16861 tggtcaatac ccactgtcat tccgaccaca tgggcggcaa tgccgcggtg cgccgggcgc
    16921 atgggtgtcg cctgagcatt cccatcggcg aagcgccgct gatcgaagcc tgggacgagc
    16981 aggccctctg gctttcctac gccgaccagc gctgcgagcg cttcgaattc gacgacacca
    17041 tcgccgccgg ggaggtgctg gactggggcg acctgcgatg gcgcgccatc gcggcacccg
    17101 gccacgacat gggcgcgctg atgttccatt gcgaggaaga acggttgctg ataaccggcg
    17161 acgccttatg ggaaaacggc ttcggcgtgc tgctgcccgg gccggatcgg gagcagcggc
    17221 tcgccgctac ccgcgccact ctggaggcga tctccagact tgacgtccac accgtgattc
    17281 cgggtcacgg cgcatctttc gccggcgtgg atgcggcgct ggaacgcagc ctgcggcggt
    17341 tggaagcgtt cgagcgcgac gaattgcgca tggcgcgtca tgtgctgaag gtgatgttcg
    17401 tgttctccct tctggatcgg caacgtctgc cgctcggccg gattcccgaa tacctggcga
    17461 cggttccgct gtacgcggat ttcaacgcac gctattttca gctcaacgat gcggcgctgg
    17521 ccgaattgat cgtcggcgac cttgaacgcg ccggtgccgt aaagaaacta aatggatatc
    17581 tgattgccgc gatttgatcc agccggccgc gggccggcga taagagcgtt cgtcgcaaag
    17641 ggcggaaaga agaaaggagc tataagactg ttctgtggat ttcgaatgat tcaccaacaa
    17701 cgcatccacc gccaaggacg ccagggaaag gcaaatgcac aaaacaggtg tgtgctaagc
    17761 aattgcacaa gtaactgggc tgcgaaaagc ccgtgccgct tactgtcgcc ctcgccttcc
    17821 tgccattttt ttcctcgcgt cctcggcgtc cttggcggtt aacgcttggc agtactggtt
    17881 tggcgacgag cgggttcttt cgcgagcagg tcaatcctct ggttaccggt catctattgc
    17941 ccataagaat gtccgaacaa ccgaagaaag aaaggcgcaa agaaagatcg cgcaagcggg
    18001 ggaaaaaaac gggcgcatca tctgcgcccg tacgcaatac gcgtaagaac atggctagga
    18061 actgcagggg acacgggcag ttggcggccc gatccttggt ctgcgtacag attattgccc
    18121 gccacagccg ctcgatgttg ttaaaaattg taaatgcagg actggccgat cactacagga
    18181 aatcgtgggc gcggccgtaa ctcgaaatgc cggtcaggac ccgcagcacg gcataagccg
    18241 ccccggtggc catccgcatc ggcaggccca ttcgcgccca gcgttcgcgc tgcaccggct
    18301 tcgctccggc ctcgatcgcg tccatcaagc tgccgtgcaa ttcgtgcacg aacgtccgat
    18361 ccaccaccac gacgttcgcc tcacgggcca gcagcaggct gacaggatcg atgttggacg
    18421 agcccacggt ggcccaggag tcatccacca cggcgacttt ggcgtgcagg aaactgcggg
    18481 tgtactcgtg gatctgcact ccggcgtcga ggagctggcg gtacagggcg cgggaagcat
    18541 aatgcaccag gatatattcc acccttccct gcagcagcag cgtcacccga acgccgcgcc
    18601 gggcggcgtc gcatagcgcg ttgcgaaaac gcctgcctgg aaggaagtag gcgttggcga
    18661 tcaccacttc gcgatgcgcc cgctcgatag cgtgcagata ggcttgctcg atatcgtgtc
    18721 gatggcggac attgtcccga accagaaatg ccgagtgctg gtcgccgcaa ggctccaccg
    18781 tcaatcgggg tttggcgaga ggcagccaac ggcgccgcag ttgtgtccaa gcggtgcgca
    18841 tccacaaagt gcgcacggcg gcgtgcatgt cggcaatcag cgggccttcg acgctcaccg
    18901 catagtccag gcgcggcgct gtgtttccgc gcgaatccct gtgatcgatg atgttgatgc
    18961 caccgcagaa gccgatgcgt ccgtccacca ccaccagctt gcggtgcagc cgccgcaggc
    19021 gcgtggtcct gagtctgaac gtccacactt cggggcggaa caccagcagc tgcacgcccg
    19081 cctccagtag ttgccggcgc cggtcttcgg gaaacaggcg cgatcc
//
LOCUS       DASQOP010000076         8330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_29676, whole genome shotgun sequence.
ACCESSION   DASQOP010000076 DASQOP010000000
VERSION     DASQOP010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8330
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29676"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..877
                     /locus_tag="VE030_02970"
     CDS             104..877
                     /locus_tag="VE030_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZF22409.1"
                     /translation="MDLELNGRAVLVTGGSKGIGLACARAFLDEGARVAVVSRDRANL
                     DAAVAQLPNVKTIVADLTGAEDAARMAQEAQRALGPIDVLVNSAGAAKRYPPDELNAQ
                     AWHAAMDAKYFTYIHAMDAVLPSMRSRRRGAVVNIIGTGGKVATPIHLPGGAANSALM
                     LASVGLASVYGKFGVRINAINPGATMTGRIREALALEAKAKGVAVNKVLEDGQSQVPL
                     GRYADPADIARVALFLASDLAAYVTGAIIPMDGGLNPII"
     gene            892..2355
                     /gene="ubiD"
                     /locus_tag="VE030_02975"
     CDS             892..2355
                     /gene="ubiD"
                     /locus_tag="VE030_02975"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011402092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HZF22410.1"
                     /translation="MHYHDLRDFIAQLEKQGELKRVSVEVSPNLEMTEICNRVLKAQG
                     PAILFEKPKGFDIPVLGNLFGTPGRVAMGMGEESVAALKEIGKLLAYLREPDPPQGLK
                     DAWDKLPVLKQVLNMAPKVVSGAPCQEIVWEGAAVDLSRLPVQTCWPGDAGPLITWGL
                     TVTRGPHKPRQNLGIYRQQVIARNRVIMRWLAHRGGALDFRDHGLAHPEKPFPVAVAL
                     GADPATILGAVTPVPDTLSEYQFAGLLRGSKTELTKCIGSDLQVPASAEIVLEGCIQP
                     GDTALEGPFGDHTGYYNEQEEFPVLTVERITMRRNPIYHSTYTGKPPDEPAILGVALN
                     EVFVPLLTKQFPEIVDFYLPPEGCSYRIAVVSMRKQYPGHAKRVMFGIWSYLRQFMYT
                     KFIIVVEDDIDVRDWKEVIWAITTRVDPARDLLIADNTPIDYLDFASPVSGLGSKLGL
                     DATNKWPGETARAWGKPIAMEGRVKQRIDRIWSELGL"
     gene            2358..4412
                     /locus_tag="VE030_02980"
     CDS             2358..4412
                     /locus_tag="VE030_02980"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HZF22411.1"
                     /translation="MPNRLAEETSPYLQQHADNPVDWHPWGEEALRLAREQDKPILLS
                     VGYSACHWCHVMAHESFEDPQIAATMNRLFVNIKVDREERPDLDQIYQTAHHMLTQRA
                     GGWPLTMFLTPGQKPFFGGTYFPKSPRYGLPGFGQLLEQVAQVYRGKRAEIDQQNESL
                     LEFMSSTLPAPPGEVMLSAAPIAAAAADLRASFDEVHGGLGSAPKFPHPFELAFCLRA
                     AASNGDTRLRDLVLLTLDKMAQGGIYDQIGGGFCRYSVDQYWLIPHFEKMLYDNGPLL
                     KLYVDAWLIAGNPLFARVCEETAAWVMREMQSPEGGYYSSLDADSEHEEGKYYVWSRE
                     EASALLNEDEIAVVFPHYGLERPPNFENHAWNLHVARPLQAVAQQLAREPAECERLLQ
                     SARRKLLAAREKRVRPGRDDKILTSWNALMIEGMVHAARAFGREDWLASAQRALDFLS
                     TRMWKEGRFLATYKDGRAHLNAYVDDYAFLLAATLEMLQAEFRKPDLEFATGVADALL
                     EHFEDRDAGGFFFTSHDHEQLILRPKPGHDNATPSGNGVAALALTRLGHLLGETRYLR
                     AGEATVKLFYPAMAQHPAGFASLGTALAEVLEPTRTVVLRGPPQPLREWQQALSRLYL
                     PDTLTLVLDSATGSLPGMLDKPAGAAVNAYLCRGVNCLAPLRDLDELLRNLRSGPMIQ
                     NG"
     gene            4488..4811
                     /locus_tag="VE030_02985"
     CDS             4488..4811
                     /locus_tag="VE030_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZF22412.1"
                     /translation="MKKFLMVIAAAAGVALASGTNAAVDVAKAKALAQSKNCLACHAV
                     DKKLVGPAYVEIAKKYKGNKDAEGMLMKKVINGGGGVWGTIPMPPNPVKDDEAKLLVE
                     WILSL"
     gene            4981..6219
                     /locus_tag="VE030_02990"
     CDS             4981..6219
                     /locus_tag="VE030_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HZF22413.1"
                     /translation="MNSEISGVSRRIVAVVAAIALAAGLGGCGKQEDQAKTGSSLERV
                     VKIGHAAPLTGEIAHLGKDNENGARLAIEEANAKGLTIGGQKVVFEFLSEDDEGKENK
                     GPIIAQKFVDAKVAGVVGHLNSGVTIPASVVYNEAGIPMISGSATNPKLTERGLKVAF
                     RTVGRDDQQGPAIASYLNAQFKPRTVAVVDDATSYGEGLADQVELTLKAAGVSVLPRE
                     KGTKDTKDWKAILTKLKGKKPDAIFYGGMDSAGGPLIKQGRELGIKAVFAFGDGACSE
                     KMHELAGAAAEGMVCSQAGIPALAASKGFLDAFKKRFNSDPIIYAPFTYDAANLLIAS
                     MQKADSADPAKYLPELAKISYEGASGRIQFDAKGDREDAEMTIFTMKAGKVVPVAIIK
                     GGNSVKFEDYVKAAESGMKK"
     gene            6353..7342
                     /locus_tag="VE030_02995"
     CDS             6353..7342
                     /locus_tag="VE030_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZF22414.1"
                     /translation="MDIFLQQVVNGLTLGSIYAVVALGYTMVYGIIQLINFAHGEVVM
                     VGAMVALATIATLAGSTALPPAVVVFAGVACAVPVCMVLGFVMERVAYRPLRGAPRLA
                     PLITAIGLSIILQHVALLIWGRSPLSFPQIMENPIFEFAGAHISMVQIGIMLISVVMM
                     VGLTLVVYRTRLGVAMRATSENPQIAGLMGIDVNRIISLTFVIGAALAAMAGVMVGTY
                     YGVAHYTMGSLLGLKAFSAAVLGGIGNLAGAMLGGILLGVVEALGAGYIGEFTDLCRY
                     SWLADLLAERCAQNQHFVLFGSNYQDVFAFLVLIAVLIFRPSGLLGERVGDRA"
     gene            7408..>8330
                     /locus_tag="VE030_03000"
     CDS             7408..>8330
                     /locus_tag="VE030_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZF22415.1"
                     /translation="MALIAASLLVLPFALASVGTAWVRITNLAILFALLSLGLNIVVG
                     FAGLLDLGYIAFYAVGAYCYALLASPHFGLHLPFWLILPIGAAVACLFGVLLGAPTLK
                     LRGDYLAIVTLGFGEIVRIFLNNLSQPVNITNGPQGVTPIDPFTLGAFSFSRSDTLLG
                     LNFSGPIKYYYLLLLVLLAVIVVNLRLQDSRLGRAWVAIREDEVAAQSMGINTRNVKL
                     LAFAMGASFGGVAGGMFAAMQGFISPESFVLVESIMVLSMVVLGGMGNVWGVILGAIL
                     LSFVPEILRYTVEPVQKAVFGHMIVEPEVIR"
BASE COUNT         1550 a         2472 c         2720 g         1588 t
ORIGIN      
        1 gtttttgccg gcctggtggc gagtttcctg ttcccgaagc cgttctgaga gttgttgtgc
       61 gcaggtgctg tgcctatagt ggcaaataca cttcgaactt accatggatc ttgaactgaa
      121 cggacgcgcg gtactcgtca ccggcggcag caaaggcatc ggcctggcct gcgcacgggc
      181 atttctcgac gaaggtgcaa gagtcgcggt cgtttcgcgc gatcgcgcaa acctggatgc
      241 ggcagtggcg cagttgccga acgtgaagac gatcgtcgcc gacctcacgg gcgcagagga
      301 cgcggcacgc atggcgcagg aagcccagcg tgcgctcggc ccgatcgacg tgctggtgaa
      361 ttcggcaggc gcggcaaagc gctacccgcc ggacgaattg aatgcacagg cctggcatgc
      421 ggcgatggat gccaagtact tcacctacat ccacgcgatg gatgcggtgc tgccatcgat
      481 gcgcagccgc cggcgcggcg cggtcgtcaa catcattggc accggcggca aggtggcgac
      541 gccgattcac ctgccgggcg gtgcggccaa ttcggcgctg atgctcgcct ccgtggggct
      601 cgccagcgtt tacggaaagt tcggggtgcg catcaacgcc atcaatccgg gtgcgaccat
      661 gaccggccgg atcagggaag cgctggcgct ggaggcgaaa gcgaagggcg ttgccgtcaa
      721 caaggtgctc gaagacgggc aatcgcaggt gccgctgggt cgctacgccg atccggcgga
      781 cattgcgcgg gtcgcgctgt ttctcgcttc cgaccttgcg gcgtatgtga ccggcgccat
      841 cattccgatg gacggcggac tcaacccgat catctgagcc ggcgttccag catgcactat
      901 cacgatctcc gcgacttcat cgcccaactg gaaaaacagg gtgagctcaa gcgcgtcagc
      961 gtcgaagtct cgccgaatct ggaaatgacc gagatctgca accgcgtcct caaggcgcag
     1021 ggtccggcga tcctgttcga gaagccgaaa ggtttcgaca tcccggtgct gggcaatcta
     1081 ttcggcacac ccgggcgcgt ggcgatgggg atgggcgagg aatcggtcgc ggcgctgaag
     1141 gaaatcggca aactgctggc ctacctcagg gagccggacc cgccgcaggg actcaaggac
     1201 gcctgggaca agctgccggt gctgaaacag gtcctgaaca tggcgccgaa agtggtgtcg
     1261 ggagcgcctt gccaggaaat cgtctgggag ggtgccgcgg tcgatctgtc caggctgccg
     1321 gtccagacct gctggccggg ggacgccggg ccgctgatca cctggggatt gacggtcacg
     1381 cgcgggccgc acaagccgcg acagaacctc ggcatctacc gccagcaggt gatcgcgcgc
     1441 aacagggtga tcatgcgctg gctggcgcat cgcggcggcg cgctggattt tcgcgaccat
     1501 ggcctcgccc acccggagaa gccgttcccg gtagcggtgg cgctcggtgc cgatccggcc
     1561 accatcctcg gcgcggtgac gccggttccg gacacgcttt ccgagtacca gttcgccggc
     1621 ctgttgcgcg gatcaaagac cgaactcacc aagtgcatcg gttccgattt gcaggtgccc
     1681 gcttccgcgg agatcgtgct ggaaggatgc atccaaccgg gagacacggc gctcgaaggc
     1741 ccgttcggcg atcacaccgg ctattacaac gagcaggaag agtttccggt gttgacggtc
     1801 gagcgcatta ccatgcgccg caatccgatc tatcacagca cctacaccgg caagccgccg
     1861 gacgagccgg cgattctcgg cgtggcgctg aacgaagtat tcgtgccgct gctcacaaag
     1921 cagtttccgg agatcgtgga cttctatctg ccgccggagg gctgttcgta ccggattgcg
     1981 gtggtgagca tgcgcaagca gtacccgggg catgccaagc gcgtgatgtt cggcatttgg
     2041 tcctacctgc gccagttcat gtataccaag ttcatcatcg tggtggagga tgacatcgac
     2101 gtgcgcgact ggaaggaagt gatctgggcg atcaccaccc gcgtggatcc ggcgcgcgac
     2161 ctgttgatcg ccgacaatac gccgatcgac tatctcgact tcgccagtcc ggtgtcgggg
     2221 ctgggatcca agctgggact ggacgccacc aacaagtggc cgggcgaaac tgcgcgcgcg
     2281 tggggcaagc cgattgcgat ggaagggcgg gtgaagcaac gcatcgaccg gatctggtcg
     2341 gaactgggtc tctagtcatg cccaatcgac ttgctgagga aaccagcccc tacctgcaac
     2401 agcatgccga caacccggtg gactggcatc cctggggcga ggaggcgctg agactcgcac
     2461 gcgagcagga caagccgatc ctgctttcgg tcggctactc cgcatgtcat tggtgccatg
     2521 tgatggcgca cgagtccttc gaagaccctc aaattgcggc cacgatgaac cggctgttcg
     2581 tcaatatcaa ggtcgatcgt gaagagcggc ccgatctcga tcagatttat cagaccgcgc
     2641 accacatgct tacccagcgc gccggcggct ggccgttgac catgtttctc accccggggc
     2701 agaaaccctt cttcggcggc acctactttc cgaaatcccc gcgttacggc ttgcccggtt
     2761 tcggtcagtt gctcgagcag gtggcgcagg tctatcgcgg caagcgcgcg gagatcgatc
     2821 agcagaacga atccctgctc gagttcatga gcagcacgtt gccggcgccg cccggcgaag
     2881 tcatgctcag cgcggcgccg atagctgctg cggccgcgga tctgcgcgcg agtttcgacg
     2941 aagtgcatgg gggtctgggc agcgccccga aattcccgca ccccttcgaa ctcgccttct
     3001 gccttcgggc ggccgcgtcg aacggtgata cgcggctgcg cgatctggtt ttgctcacgc
     3061 tcgataaaat ggcgcagggc gggatctacg accagatcgg cggcggattc tgccgctaca
     3121 gcgtggatca gtactggctg atcccgcact tcgagaaaat gctttacgac aatgggccgt
     3181 tactgaagct ttatgtggac gcctggctca tcgccggcaa tccgttgttc gcgcgcgtat
     3241 gcgaggagac tgcggcctgg gtgatgcgcg agatgcaatc tccggagggc ggctactatt
     3301 cgtcgctcga cgctgattcg gagcacgagg aaggcaagta ctacgtctgg tcacgcgagg
     3361 aagcgagtgc gctcctgaac gaagacgaga ttgcggtggt gttcccgcac tacggcctgg
     3421 agcggccgcc gaatttcgag aaccatgcct ggaacctgca tgttgcgcgg ccgctccagg
     3481 cagtggcgca gcaattggcg cgcgagccgg ccgaatgcga gcgcctgctg caaagcgcgc
     3541 gcaggaagct gctcgctgcg cgcgagaaac gcgtgcgccc gggccgggac gacaagatcc
     3601 tcaccagctg gaatgcgctg atgatcgagg gcatggtaca tgcggcacgg gcattcgggc
     3661 gcgaagattg gctggcctcc gcgcagcgcg cgttggattt tctgagcacc agaatgtgga
     3721 aagaggggcg gtttctagcg acatacaagg acggtcgcgc ccacctcaac gcctatgtag
     3781 acgactatgc cttcctgctg gccgcgacgc tggagatgct gcaggcggaa ttccgcaaac
     3841 cggacctcga gttcgcgacc ggggttgccg atgcgctgct cgagcatttc gaggatcgtg
     3901 acgcaggcgg atttttcttc accagtcacg atcacgagca gctgatcctc cggcccaaac
     3961 ccggccacga caatgctacg ccctcgggaa acggcgttgc ggcactagcg ctcacccggc
     4021 ttggccatct tcttggcgaa acccgctatc tgcgcgcagg cgaggccacg gtgaagctgt
     4081 tctacccggc gatggcgcag cacccggccg gatttgcgtc tctcggcacc gcgctcgccg
     4141 aggtgctgga accgacacgg acggtggtgc tgcgcggccc gccccagccc ttgcgcgagt
     4201 ggcaacaggc gctgtcccgg ctctacctgc cggatacgct gactctggtc ctggattcgg
     4261 cgaccgggag cctccccggt atgctggaca agcctgcggg cgcggctgtc aacgcctatt
     4321 tatgccgcgg cgttaattgc ctcgcacccc tccgggatct cgacgagctg ctcaggaatc
     4381 tgcgaagcgg tccaatgatc caaaatggtt gacggtttcc aaggcgggcc aaaagccgat
     4441 aaaatgtccg gcgcttcaat tcaacctgta acgaaaggca cgagcaaatg aaaaaattcc
     4501 tcatggtgat cgcggcggca gctggtgtgg cgcttgcgag cgggacgaac gcggcggtgg
     4561 acgtcgccaa ggccaaggcg ctggcacagt cgaagaattg cctggcctgt cacgcggtgg
     4621 acaagaaact ggtgggcccg gcttacgtcg agatcgccaa gaaatacaag ggcaacaagg
     4681 atgccgaggg catgctgatg aagaaagtca tcaacggcgg cgggggcgtg tggggtacca
     4741 ttccgatgcc gcccaatccg gtcaaggacg acgaggccaa gcttctggtg gagtggatcc
     4801 tgtcgttgta gatcggtggt tcacgcgaac cacgggaaag gccggcaatc gccggctttt
     4861 tttgtttctg ccggtcacga caacccgatg aattacgggc gcaaattgac aatgttttga
     4921 agcgtggccg ataatcgctg gagggtttcc agtgcgctta tatggagggg gacaggaatc
     4981 atgaacagcg aaatctctgg ggtctcgcgg cgaatcgtcg cagtggtggc cgcaatcgcg
     5041 cttgcggctg gcttgggcgg gtgcggcaag caggaggacc aggccaagac cggctcgtcg
     5101 ctcgagcgtg tcgtcaagat cggccacgcg gcgccgctga cgggcgaaat tgcccacctg
     5161 ggcaaggaca atgaaaacgg cgcgcgcctt gcgatcgagg aagccaatgc caagggcctg
     5221 acaatcggcg gccagaaagt ggttttcgaa ttcctgagcg aggacgacga aggcaaggaa
     5281 aacaagggtc cgatcatcgc gcagaaattc gtggatgcga aagtcgccgg cgtggtcgga
     5341 cacctgaact ccggcgtgac catccccgcc tcggtggttt acaacgaagc cgggattccg
     5401 atgatcagcg gctccgcgac caatcccaag cttaccgaac ggggcctgaa ggtggctttc
     5461 cgcaccgtcg gccgcgatga tcagcaaggt ccggcgatcg ccagctacct caacgcccaa
     5521 ttcaaaccca gaaccgttgc cgtcgtcgac gatgccactt cgtacggtga agggctcgcg
     5581 gaccaggtcg agttgaccct gaaggcagcc ggcgtaagcg tgttgccccg ggagaaaggt
     5641 accaaggaca ccaaggattg gaaggcgatt ctgaccaagc tgaaaggcaa gaaaccggat
     5701 gccattttct atggcggcat ggattccgcg ggcgggcctt tgatcaagca gggacgcgag
     5761 ctaggcatca aggcggtatt cgctttcggc gatggtgcct gctcggagaa aatgcacgaa
     5821 cttgcgggcg cggctgccga gggcatggtc tgctcgcagg ccggcattcc agccctggcc
     5881 gccagcaagg gcttcctcga cgcattcaag aaacgcttta attccgatcc gatcatttac
     5941 gcaccgttca cctacgatgc cgcgaatctc ctgatcgcat ctatgcagaa ggccgactct
     6001 gcagatcccg ccaagtacct cccggaactg gcgaagatca gctacgaagg cgcgagcggc
     6061 agaatccagt tcgacgccaa gggcgatcgc gaggacgccg agatgacgat ctttaccatg
     6121 aaagcaggca aggtcgtgcc ggtggcgatc atcaagggcg gcaattccgt caagttcgag
     6181 gattacgtga aggccgcaga atccggcatg aaaaaataac ggcggcattc cgcgagtgat
     6241 ccggatgcgc ttctgacgga acgtgaaacg gcaccttcgg gtgctgtttt cacgtgacgc
     6301 ccgcaatttc cgaaaccgga cccggtttcg gcgagcacac tacgcgacct tgatggacat
     6361 cttcctgcag caggtggtaa acggactgac gctggggagc atatacgcgg tcgtggcgtt
     6421 gggctacacc atggtctacg gcatcatcca gttgatcaac ttcgcgcatg gcgaagtggt
     6481 gatggtgggt gccatggtgg cgctggcaac gattgcgact ctagccggtt caacggcgct
     6541 accgcctgcg gtcgtggttt tcgctggcgt ggcttgtgcc gtgccggtgt gcatggtgct
     6601 cgggttcgtg atggagcgcg ttgcctaccg tccgctgcgc ggcgcacccc ggctggcgcc
     6661 gcttatcacc gcgatcggcc tgtcgatcat cctgcagcat gttgcgcttc tcatctgggg
     6721 ccgcagtccg ctgtcgtttc cgcagatcat ggagaacccg atattcgagt tcgccggcgc
     6781 ccacatcagc atggtacaga tcgggatcat gctgatatcc gtggtgatga tggtcgggct
     6841 cacgctcgtg gtgtaccgca cccgcctcgg tgtggcgatg cgggccacct ccgaaaatcc
     6901 gcagatcgcg gggctgatgg gcattgacgt caatcgcatc atctccctga cgttcgtcat
     6961 cggcgccgcc ctcgccgcga tggctggggt gatggtcggc acctactacg gggtcgcgca
     7021 ttacacgatg ggctcattgc tcgggctcaa agccttctcc gccgcggtgc tcgggggcat
     7081 cggcaacctt gccggggcaa tgctcggcgg cattctgctc ggggtggtgg aggcgctggg
     7141 cgcgggatat atcggcgagt tcaccgacct gtgccgctat tcgtggcttg ctgacctgct
     7201 tgcggagcgc tgcgcacaga accagcactt cgttctgttc ggcagcaatt accaggatgt
     7261 gttcgctttt ctggtgctga tcgcggtgct gatattcagg ccgtcgggat tgctgggcga
     7321 gagggtcggg gaccgggcgt gattttttcg catcccgccg cttggttcga acggctacga
     7381 caccacccgg cggcagcacg cgccgggatc gcgctgattg cggcgtcgct gctggtgctg
     7441 ccgttcgcgc tggcgtcagt gggcacggcg tgggtgcgca tcaccaatct tgcgatactt
     7501 ttcgcgctgc tttccctggg cctgaacatc gtggtcgggt ttgcggggct gctcgacctc
     7561 ggttacatcg cgttttatgc ggtaggcgcg tattgctacg cgctgctcgc ttcgccgcat
     7621 ttcggcctgc acctgccttt ctggctcatc ctgccgatcg gcgccgcggt tgcctgcctg
     7681 ttcggcgtgc tgctcggcgc gcccaccctg aaacttcgcg gagactatct cgccatcgtg
     7741 acgctgggct tcggcgagat cgtccgaata ttcctgaaca acctttcaca gccggtgaac
     7801 atcaccaatg ggccgcaggg cgttaccccg attgacccat ttacgctggg cgcgttcagc
     7861 ttcagccgca gcgataccct gctcggcctc aacttcagcg gcccaatcaa gtactactat
     7921 ctgctgctcc tggtcctgct ggcggtgata gtcgtcaatc tgcgcctgca ggattcccgt
     7981 ctgggacggg cctgggtggc cattcgcgag gacgaagtgg cggctcagtc aatgggcatc
     8041 aacacgcgca acgtgaagct gttggcgttt gcgatgggtg ccagcttcgg cggcgtggcg
     8101 ggcgggatgt tcgcggcgat gcagggcttc atcagcccgg agagttttgt cctggtggaa
     8161 tcgatcatgg tgctctccat ggtcgtgctc ggtggcatgg gtaatgtctg gggcgtgatc
     8221 ctaggggcga tactgttgtc gttcgtcccg gagatcctcc gctataccgt cgagccggtc
     8281 cagaaagcgg tgttcggcca tatgatcgtc gagccggagg tgatacgcat
//
LOCUS       DASQOP010000077         7614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2245448, whole genome shotgun sequence.
ACCESSION   DASQOP010000077 DASQOP010000000
VERSION     DASQOP010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7614
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2245448"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..1036)
                     /locus_tag="VE030_03005"
     CDS             complement(215..1036)
                     /locus_tag="VE030_03005"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HZF22416.1"
                     /translation="MLPKRTAFFVSDRTGITAEMLGHSLLTQFDRVSFTEITLPFVDS
                     PEKAQDAVRQIDQTAEKDGARPLVFSTLVDQAMRSAIQRANAFYIDCFQVFIQPMEAE
                     LGVQSSHTVGKSHSVRSNEYFGRIEAVNFALAHDDGQSTKDLANADVILVGVSRCGKT
                     PTCLYMALQFGIRAANYPLIPEDFDAKALPAAIRAFRSKLFGLTIRPDRLQRIRSERR
                     PGSTYASLENCEHELREAERLMRQEGISILDTTSRSIEELATTILQQANLQRHVY"
     gene            1111..3501
                     /gene="ppsA"
                     /locus_tag="VE030_03010"
     CDS             1111..3501
                     /gene="ppsA"
                     /locus_tag="VE030_03010"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008812026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HZF22417.1"
                     /translation="MSNQRLVIPFEALRMTDVESVGGKNASLGEMISQLARAGVRVPE
                     GFATTAAAYRQFVAANGLAGRIEQALSGLDVDDITALASAGAQIRKWILEAPLPKLLQ
                     SEIGAAYQALVKGQGSDASFAVRSSATAEDLPDASFAGQQETFLNIHGLDNVLLAIRH
                     VFASLYNDRAISYRVHKGFVHSAVALSAGVQRMVRSDKGAAGVMFTLDTESGFDRVVF
                     ITSAYGLGETVVQGQVNPDEFYVYKPALEAGRPAILRRNLGTKALRMVFTDSRAAGRS
                     VQTLEVPESERRQFSIGDSEVEELGRYAMIIEKHYGRPMDIEWGRDGVDGKLYILQAR
                     PETVKAREETQERFRLKKRGDVLITGRAIGNRIGTGTVRLVKNASEMNRVKPGDVLVT
                     DMTDPDWEPVMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGEATQKLAQGERVTV
                     SCAEGDTGYVYQGILDVEVLHIQLDKLPDVPVKISMNVGNPELAFDFQRLPNAGIGLA
                     RLEFIIARMIGVHPKAALAYPKVDAELKKGIETLAAGYPDPVTFYVEKLTEGIATLGA
                     AFFPKPVIVRLSDFKSNEYSSLTGGKQYEPEEENPMLGFRGASRYIADSFRDCFELEC
                     RALRKVRDQMGLTNVEIMVPFVRTVHEAEQVIALLRKNGLERGLNGLRIIMMCEIPSN
                     AILADEFLQHFDGFSIGSNDMTQLTLGLDRDSGIVAGQFDERDPAVKAMLAMAIQACR
                     RAGKYVGICGQGPSDHPDLAEWLMEQGIDTISLNPDTVIETWMFLAKHAAQQAAAA"
     gene            3590..5260
                     /locus_tag="VE030_03015"
     CDS             3590..5260
                     /locus_tag="VE030_03015"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HZF22418.1"
                     /translation="MTKFIFVTGGVVSSLGKGIASASLAAILESRGIKLTLLKLDPYI
                     NVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFVNAKMTRRNNFTTGQIYESVIKKE
                     RRGDYLGGTVQVIPHITDEIKNFIRMGAGDADVAICEIGGTVGDIESLPFLEAVRQMS
                     IQNGRGNTCFIHLTLVPYIPSAGEIKTKPTQHSVKELREIGIQPDILLCRADRMVPDN
                     ERRKIALFTNVEEEAVISAVDVDSIYKIPGFLHGQGLDEIVCRKLGIKAPPADLSKWE
                     KLLHDREHPAHEANIALVGKYVDLADSYKSLSEALIHAGIHTGTRLNLHYIDSESIEK
                     DGIGCLKDMDAVLVPGGFGKRGVEGKIAAIRYARENKVPYLGICLGMQLATIEFARNV
                     AGFAGANSTEFEHDTPHPVVALITEWLDRTGRVERRSGESDMGGTMRLGAQSCPVEPG
                     TLAAQIYGPKVTERHRHRYEVNNAYVPALEAKGYKVSARTPSENLPEMMELPDHPWFV
                     GCQFHPEFTSTPLDGHPLFSAFIRAAVNFRQGERADPVSQPAKIKSIA"
     gene            5262..6116
                     /gene="kdsA"
                     /locus_tag="VE030_03020"
     CDS             5262..6116
                     /gene="kdsA"
                     /locus_tag="VE030_03020"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HZF22419.1"
                     /translation="MKLCGFEVGLDRPLFLIAGPCVVESREMALDTAGKLKEIAAAVG
                     VPFIYKSSYDKANRSSSKSFRGLGMDTGLQILAEVRKQIGVPVLTDVHAEDEIAAAAE
                     VVDVLQTPAFLCRQTDFIRAAAMSGKPVNIKKGQFLAPGDMKNVVDKAREASGKDNIM
                     VCERGASFGYNNLVSDMRSLAIMRDTRCPVVFDATHSVQLPGGQGTSSGGQREFVPVL
                     ARAAVATGVAGVFMETHPNPEKALSDGPNAWPLHLMKPLLETLKALDELVKKRAFAED
                     ALRAKPAR"
     gene            6148..7431
                     /gene="eno"
                     /locus_tag="VE030_03025"
     CDS             6148..7431
                     /gene="eno"
                     /locus_tag="VE030_03025"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HZF22420.1"
                     /translation="MSSIVDIVAREILDSRGNPTVEADVLLESGVSGRAAVPSGASTG
                     TREAIELRDGDKGRYGGKGVLKAVENVNTEICEAIVGLDAVEQSFIDQTLIDLDGTEN
                     KSRLGANALLAVSLAVAKAAAEESGLPLYRYLGGAGRMQLPVPMMNVINGGAHANNSL
                     DLQEFMIIPVGAESFREAMRCGAEIFQTLKKLLDKKGMSTTVGDEGGFAPNLPNNEAA
                     LKLIIEAIEASGYTPGSQVAIGLDCASSEFFKDGKYDLASEKLKLSAKEFTDYLAAWA
                     DKYPIVSIEDGMAEGDWEGWKVLTNRLGKKVQIVGDDLFVTNTKILSEGIRQRVANSI
                     LIKINQIGTLTETFAAIEMAKRAGYTAVVSHRSGETEDTFIADLAVGANALQIKTGSL
                     SRSDRLAKYNQLLRIEEDLGDSASYAGRDAFYQIN"
     gene            7435..>7614
                     /locus_tag="VE030_03030"
     CDS             7435..>7614
                     /locus_tag="VE030_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HZF22421.1"
                     /translation="MRFLAFALVLLIALIQNPLWFGKGGWLRVWELDRQVDAQRDTNA
                     QLAARNAALDAEVLDL"
BASE COUNT         1516 a         2337 c         2407 g         1354 t
ORIGIN      
        1 agatgtgaaa agcgaaacgg ggagtggtcc gatcgacgga ctgaccagct cgagttggtc
       61 ccggttatga aaatgcccac cacacagatg tcggaagaat aggaacgcga aagagtccgc
      121 gtcggaccaa acaattgcag cccggcacgc gcttttttcg ctcccgtatt tttgcttttc
      181 ttcgcgtcct ttgcgtcttg gcggtgaaaa gatctcagta gacatgacgc tggagattgg
      241 cttgctgcag aatcgtggtg gccagttcct cgatcgagcg cgacgtggtg tcgaggatgg
      301 aaatgccctc ctggcgcatc agcctttcgg cctcgcgcaa ttcatgctcg caattctcaa
      361 ggcttgcgta agtgctgccc ggccggcgtt cgctacggat gcgttgcagg cggtcaggcc
      421 ggatggtcag gccgaacagt ttgctgcgaa aggcgcggat tgccgccggc agggccttgg
      481 cgtcgaagtc ctccgggatc agcggatagt tggccgcgcg aatgccgaac tgcaaggcca
      541 tgtacaggca ggtcggcgtc ttgccacagc gggatactcc caccaggatc acatcggcgt
      601 tggcgagatc cttggtgctc tggccgtcat cgtgcgccaa ggcaaagttc accgcttcga
      661 tccggccgaa atactcgttg ctgcgaacgc tgtgcgactt gcccaccgtg tgggaagact
      721 ggactcccag ttccgcttcc atcggctgga tgaagacctg gaagcagtcg atgtaaaagg
      781 cgttcgctct ctggatggcg ctgcgcatgg cctggtccac cagcgtgctg aacaccagcg
      841 gacgggcgcc gtccttctcc gcggtctggt ctatctgccg taccgcgtcc tgcgccttct
      901 ccggcgaatc cacgaaagga agcgtaatct cggtgaacga cacccggtcg aactgggtaa
      961 gcaggctgtg tccgagcatc tcggcggtga tcccggtgcg gtccgatacg aaaaatgcag
     1021 ttcgtttggg caacattgtc ttggtgcacg tgtaatgatt cagccggtaa aataacatct
     1081 tttcaacttt ctccatctgc ggacagcaga atgagcaatc aacgcctcgt tatcccgttt
     1141 gaagcactgc gcatgaccga cgtcgagagc gttggcggca agaacgcctc cctgggggag
     1201 atgatcagcc agttggcccg ggcgggtgtg cgggttccgg aaggctttgc caccaccgca
     1261 gcggcgtatc gccagtttgt ggctgcgaac ggactggcgg ggaggatcga gcaggcgctc
     1321 tccggactcg acgtcgatga catcaccgcg ctggcttctg ctggcgcgca gatccgcaag
     1381 tggatactgg aagcgccatt gccgaaactc ctgcagtcag agatcggcgc ggcgtaccag
     1441 gcactcgtca aagggcaggg cagcgatgcc tctttcgctg tgcgttcctc ggccacggcg
     1501 gaggatctgc cggacgcttc cttcgccggc cagcaggaga cctttctcaa cattcacggc
     1561 ctcgacaacg tgctgctggc catccgccac gtattcgctt cgctctacaa cgaccgcgcc
     1621 atctcttacc gggtgcacaa gggcttcgtg cattccgcag tggcattgtc ggccggcgtg
     1681 cagcgcatgg tacgcagcga caagggtgcg gccggcgtaa tgttcacgct cgataccgaa
     1741 tccgggttcg acagagtggt gttcatcact tctgcctatg gattgggcga aaccgtggtg
     1801 caggggcagg tcaaccccga tgagttctac gtttacaagc cggcgctgga ggccggccgt
     1861 ccggcgatcc tgcgccgcaa tctaggcacc aaggcgctgc gcatggtgtt caccgacagc
     1921 cgcgctgccg gccgctcggt gcagacgctc gaggtgccgg agtccgaacg caggcaattc
     1981 tcgatcggag acagcgaggt cgaggagctc ggccgttacg cgatgatcat cgagaagcac
     2041 tacggccggc cgatggacat cgaatggggc cgcgacggag tcgatggcaa gctctacatc
     2101 ctgcaggctc gccccgaaac ggtgaaggcg agggaggaaa cgcaggagcg cttccgcctg
     2161 aagaaacgcg gcgacgtgct gatcaccggc cgcgcgatcg gcaaccgcat cggcaccggc
     2221 acggtgcggc tggtgaagaa cgcctccgag atgaaccggg taaagcccgg cgacgtgctg
     2281 gtgaccgaca tgaccgaccc ggactgggag ccggtgatga agaaggcctc ggccatcgtc
     2341 accaaccgcg gcggacgcac ctgtcacgcc gccatcatcg cccgcgaact cggcattccc
     2401 gcggtggtag gctgcggcga ggccacgcaa aagctggcac agggcgaacg cgtgaccgta
     2461 tcctgcgcgg aaggcgatac cggttacgtc taccagggaa tcctggacgt agaagtgctc
     2521 catattcagc tcgacaaatt gcccgatgtg ccggtgaaga tcagcatgaa cgtcggcaat
     2581 ccggagctgg cattcgattt tcagcggctg cccaacgccg gtatcggcct ggcgcggctg
     2641 gagttcatca tcgcccgcat gatcggcgtg caccccaaag cggcgctggc ctacccgaag
     2701 gtcgatgccg agctgaagaa ggggatcgag acactggcgg caggctatcc ggacccggtg
     2761 actttctatg tcgaaaagct caccgaaggc atcgccacgc tgggggcggc gttctttccc
     2821 aaaccggtga tcgtgcgcct gtcggatttc aagtccaacg agtattcgag cctcaccggc
     2881 ggcaagcagt acgagccgga ggaagaaaac ccgatgctgg gctttcgcgg cgcctcgcgc
     2941 tacatcgccg attcctttcg cgactgcttc gagctggaat gccgtgcgtt gaggaaggtg
     3001 cgcgaccaga tgggcttgac caacgtcgag atcatggtgc ctttcgtgcg taccgtgcac
     3061 gaagcggagc aggtgatcgc gctgctaagg aagaacggcc tggagcgtgg cctcaacggc
     3121 ttgcgcatca tcatgatgtg cgagattcct tcgaatgcca tcctcgccga cgagttcctg
     3181 cagcacttcg acggcttttc gatcggctcc aacgatatga cccagctcac gctcggcctg
     3241 gatcgcgatt cgggcatcgt tgccgggcag ttcgacgagc gcgatccggc ggtgaaggcg
     3301 atgctcgcca tggccatcca ggcctgccgc agggccggca agtatgtcgg catctgcgga
     3361 caggggcctt ccgaccatcc cgatctggcg gaatggctga tggaacaggg catcgacacg
     3421 atttcgctga atcccgacac ggtgatcgaa acctggatgt tcctggccaa gcacgccgcc
     3481 caacaggcgg ccgcggcctg acttgaataa tgtccgggca gcacaatatt cgtaacgccc
     3541 ggcttgcctt tgggttatca ttcggcaggc aagtcggagc gggtatggaa tgaccaaatt
     3601 tatctttgtc accggcggcg tggtctcttc cctgggcaaa ggtatcgctt ccgcgtctct
     3661 cgccgcaatt ctcgaatccc gcggcatcaa gctcaccctc ctcaagctcg acccctacat
     3721 caacgttgat cccggcacca tgagcccctt ccagcacggg gaggtgttcg tgaccgagga
     3781 cggcgccgag accgatttgg atctcgggca ttacgagcgc ttcgtcaacg ccaagatgac
     3841 ccggcgcaac aatttcacca ccggccagat ctacgagagc gtcatcaaga aggagcgccg
     3901 cggcgactat ctcggcggca cggtgcaggt gattccgcac atcaccgacg agatcaagaa
     3961 cttcatccgc atgggcgcgg gcgacgccga cgtggcgatc tgcgaaatcg gcggcaccgt
     4021 cggcgacatc gaatccctgc cgttcctgga agcggtgcgg cagatgagca tccagaacgg
     4081 ccgcggcaac acctgcttca tccacctgac gctggtgccc tatatcccgt ccgccggcga
     4141 gatcaagacc aagcctacgc agcactctgt caaggaactg cgcgaaatcg gcatccagcc
     4201 cgatatcctg ttgtgccgcg ccgatcgcat ggtgccggac aacgagcgcc gcaagatcgc
     4261 gctgttcacc aacgtcgagg aagaagcggt gatctcggcc gtggacgtcg attcgatcta
     4321 caagattccc ggtttccttc acgggcaggg tctcgacgaa atcgtctgcc gcaagctcgg
     4381 catcaaggcg ccgccggccg atctctccaa atgggagaag ctgcttcacg accgcgagca
     4441 tcccgcgcac gaggcgaaca ttgcgctggt cgggaagtac gtcgatctcg ccgattctta
     4501 caaatcgctt tcggaagcgc tgattcacgc cggcatccat accggcacgc ggctgaacct
     4561 ccactacatc gattccgaaa gcatagaaaa ggacggcatc ggctgcctga aggacatgga
     4621 cgccgttctg gtcccgggcg gtttcggcaa gcgcggcgtc gagggcaaga tcgcggccat
     4681 ccgttacgcg cgcgagaaca aggtgcctta tctgggcatt tgcctgggca tgcagctcgc
     4741 gaccatcgag ttcgcgcgca acgtcgccgg atttgccggc gccaacagca ccgaattcga
     4801 gcacgatacg ccgcatccgg tggttgcgct catcaccgag tggctggacc gcaccggccg
     4861 ggtcgagcgc cgcagcggcg aatccgacat gggcggtacc atgcgcctcg gcgcgcagtc
     4921 ctgcccggtc gagcccggca cgctggccgc acaaatctac gggccgaagg ttaccgagcg
     4981 ccaccgccac cgctatgagg tcaacaacgc ctacgtgccg gcgctcgagg cgaagggcta
     5041 caaggtttcc gcgcgcacgc cgagcgagaa cctgccggaa atgatggaac tgccggacca
     5101 tccctggttc gtcggctgcc agttccaccc cgaatttact tccacgcccc tcgacggcca
     5161 tccgctgttt tccgctttca tccgcgcagc cgtgaatttt cgccagggcg agcgggccga
     5221 cccggtttcg cagccggcca agatcaagag catcgcgtga catgaaactc tgcggcttcg
     5281 aagtcggcct tgaccggcct ctatttttga tagccgggcc gtgcgtggtc gaatccaggg
     5341 aaatggcgct ggataccgcg ggcaagctca aggaaatagc cgcggcggtc ggcgtgccct
     5401 tcatctacaa gtcctcgtac gacaaggcca atcgcagctc cagcaagtcg ttccgcggcc
     5461 tgggcatgga caccggcttg cagatactcg ccgaggtgcg caagcaaatc ggcgtgccgg
     5521 tgctgaccga cgtgcacgcc gaggacgaga tcgcggcggc cgcggaggtg gtggatgtgc
     5581 ttcagacccc ggccttcctg tgccgccaga ccgatttcat ccgcgccgca gcgatgagcg
     5641 gcaagccggt caacatcaag aaggggcagt tcctcgcccc cggcgacatg aagaacgtgg
     5701 tggacaaggc gcgcgaggcc agcggcaagg acaacatcat ggtgtgcgaa cgcggtgcct
     5761 cgttcggcta caacaatctg gtttccgaca tgcgttcgct ggcgatcatg cgcgacaccc
     5821 gctgcccggt ggtgttcgat gcgacccatt cggtgcagtt gccgggcggg cagggcacct
     5881 ccagcggcgg ccagcgcgag ttcgtgccgg tgctggcgcg cgcggcggtg gcgaccggcg
     5941 tggccggggt gttcatggaa acgcatccga acccggagaa ggcgctttca gacggaccca
     6001 acgcctggcc gttgcatctg atgaagccac tgctggaaac cctcaaggcg ctggatgaac
     6061 tggtcaagaa gcgcgccttt gccgaggacg cgctgagggc gaaaccggcc cgctaaccaa
     6121 caagcagaac accaagaagg aagaagaatg agttcgatcg tcgacatcgt ggcccgcgag
     6181 attctggatt cgcgaggcaa tccgaccgtg gaagccgacg tgctgctgga gtcgggcgtg
     6241 agcggccggg cggcggtgcc ttcgggcgca tccaccggca cgcgcgaagc catcgagttg
     6301 cgcgacggcg acaagggccg ctatggcggc aaaggcgtgc tcaaggcggt ggagaacgtc
     6361 aataccgaga tctgcgaagc gatcgtgggc ctggacgcgg tggagcagag cttcatcgac
     6421 cagacgctca tcgacctcga cggcaccgag aacaaatcgc ggctcggcgc caacgccctg
     6481 ctcgcggtct cgctggcggt ggccaaggcg gctgcagaag agtccggact gccgctgtac
     6541 cgctatctcg gcggggccgg caggatgcag ctgccggtgc cgatgatgaa cgtgatcaac
     6601 ggcggggcgc acgccaacaa cagtctcgac ctgcaggagt tcatgatcat cccggtgggt
     6661 gcggagagtt tccgcgaggc gatgcgctgc ggcgcggaga ttttccagac attgaagaaa
     6721 ttgctcgaca agaaaggcat gtccacgacg gtgggcgatg aaggcggctt tgcgcccaac
     6781 ctgccgaaca acgaagcggc gctcaagctc atcatcgaag cgatcgaggc ttccggatac
     6841 acgcccggct cgcaggttgc aatcggactc gattgcgcca gctcggagtt cttcaaggac
     6901 ggcaaatacg accttgcctc ggagaagctg aaactgagcg ccaaggagtt caccgactat
     6961 ctggcggcat gggcggacaa gtacccgatc gtcagcatcg aggacggcat ggccgaaggc
     7021 gactgggagg gctggaaggt cctgaccaac aggctcggca agaaagtcca gatcgtcggc
     7081 gatgacctgt tcgtcaccaa caccaagatc ctttccgaag gcatacgcca gcgcgttgct
     7141 aattcgatcc tcatcaagat caaccagatc ggtacgctga ccgagacctt tgccgcgatc
     7201 gagatggcca agcgcgcggg ctacacggcg gtggtgtcgc atcgttccgg cgaaaccgag
     7261 gacacgttca tcgccgatct cgcggtcggc gccaacgcac tgcagatcaa gaccggctcg
     7321 ctgtcgcgtt ccgaccggct ggcgaagtac aaccagttgc tgcgcatcga ggaagacctc
     7381 ggcgactcgg ccagctacgc cggccgtgac gccttctatc agatcaatta accgatgcgc
     7441 ttcctcgcct ttgcactggt cctgcttatc gcgctgatac agaacccgct gtggttcggc
     7501 aaaggcggtt ggctgcgtgt ctgggaactc gaccgccaag ttgacgcgca gcgcgacacg
     7561 aatgcgcagc ttgccgcgcg caatgccgcg ctcgatgccg aagtcctgga tcta
//
LOCUS       DASQOP010000078         3242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1641189, whole genome shotgun sequence.
ACCESSION   DASQOP010000078 DASQOP010000000
VERSION     DASQOP010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1641189"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3036
                     /gene="purL"
                     /locus_tag="VE030_03035"
                     /gene_synonym="purI"
     CDS             <1..3036
                     /gene="purL"
                     /locus_tag="VE030_03035"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HZF22422.1"
                     /translation="YVTEPTHILMKVETHNHPTAISPFPGAATGSGGEIRDEGATGRG
                     AKPKAGLTGFSVSHLRIPGFAQPWERGGVGKPGRIASALQIMLEGPVGGASFNNEFGR
                     PNLCGYFRTFEQTVNGAVRGYHKPIMLAGGVGAISASQSHKAVVKPGALLVQIGGPGL
                     LIGMGGGAASSMDTGANIEDLDFDSVQRGNAEIQRRAQEVIDRCWAMGEANPILSIHD
                     VGAGGLSNALPELVHGAGRGGHVDLRKIPSEEPGMTPMQVWCNEAQERYVMAIAGERF
                     DAFEALCMRERCPFSVVGKVTEDGWLKVLDPLFGNHPVDMDLEVVFGKPPKMTRDVAH
                     RAQKPASLDFSAVTLTDAAQRVLRFPAVADKTFLISIGDRTVGGLCARDPFVGPWQVP
                     VADVAVTLMGFRTDRGEAFAMGERTPLAVIDAPASGRMAVGEAITNIAAARIAKLGDV
                     KLSANWMAAAGHPGEDAALFDTVEAVAMELCPQLGISIPVGKDSMSMRTSWEDDGRKK
                     EVVAPLSLIVSAFAPCLDARKTLTPQLQPLDRDSMLVLIDLGSGRNRLGGSVLAQVYS
                     QIGDEAPDLDSPRKLKALFDTVQQLSSRGSILAYHDRSDGGLFATLAEMMFASHVGIT
                     ADIGALGEDALAALFNEELGAVLQVKTSDFDAVKGAFSRAGLGAELHRLGIINRAGRL
                     IIVAKGKEIYSESGIALQRAWSETTYHMQRLRDNPQCAQQEYDRILDAADPGLHAKLT
                     YDPAQDVAAPYIGRGARPKMAILREQGVNGQIEMAAAFDRAGFETRDVHMSDIIAGRA
                     SLAGFKGVVACGGFSYGDVLGAGEGWAKSILFNRRARDEFEAFFNRGDSFALGACNGC
                     QMMSNLHEIIPGTAHWPHFVRNRSEQFEARVVMLEVMKSPSLFFRGMEGSRMPIAVAH
                     GEGYAEFASPGQLAATRGLVALRFVDNAGRPTERYPFNPNGSPQGITGLTTVDGRFTI
                     LMPHAERVFRTAQNSWHPDSWGEDGAWMRMFRNARKWVG"
BASE COUNT          582 a          983 c         1102 g          575 t
ORIGIN      
        1 tatgtcaccg agcctaccca tatcctgatg aaagtggaga cgcataatca cccgaccgcc
       61 atttcgcctt ttccgggtgc cgcgaccggc tcgggcggcg agatccgcga cgagggcgca
      121 accggccgcg gcgccaagcc gaaggccggg ctaaccggat tctcggtttc gcacttgcgc
      181 atccccggtt tcgcgcaacc ctgggaaagg ggcggcgtcg gcaaaccggg ccgcatcgca
      241 tcggcattgc agatcatgct cgaggggccg gtcggtggtg cgtccttcaa caacgagttc
      301 ggccgcccga atctttgcgg ctatttccgc accttcgagc agacggtgaa cggcgcggtg
      361 cgcggctacc acaagccgat catgctggcc ggcggcgtag gcgcgatatc cgcttcgcaa
      421 tcgcacaagg ccgtggtgaa gcccggcgcc ctgctggtgc agatcggcgg tccgggtttg
      481 ctgatcggca tgggcggggg cgccgcttcc agcatggaca ccggagcgaa catcgaggat
      541 ctcgacttcg attcggtgca acgcggcaac gccgaaatcc agcgccgcgc gcaggaggtg
      601 atcgaccgct gctgggcgat gggcgaagcc aatccgattc tttccataca cgatgtcggc
      661 gccggcggat tgtcgaacgc gctgccggaa ctggtgcacg gcgccggccg cggcgggcac
      721 gtcgatctca ggaagattcc ttccgaggaa ccgggcatga cgccgatgca ggtgtggtgc
      781 aacgaagcgc aagagcgcta cgtgatggcg atcgccgggg agcgcttcga tgccttcgag
      841 gccctgtgca tgcgcgagcg ttgtcctttc tccgtggtcg gcaaggtgac cgaggacggc
      901 tggctcaaag tgctggatcc gctgttcggc aaccatccgg tggatatgga tctcgaggtc
      961 gtgttcggta agccgccaaa gatgacccgg gacgtcgcac accgggcgca gaaaccggcg
     1021 tcgctcgatt tctccgcggt gacgctcacg gatgcggcgc agcgggtgct gcgtttcccg
     1081 gcggtggcgg acaagacttt cctgatttcg atcggcgacc gcactgtcgg tggcctgtgc
     1141 gcgcgcgatc cgtttgtcgg gccctggcag gtgccagttg ccgatgtggc cgtcacgctg
     1201 atgggattca ggaccgatcg cggcgaggcc ttcgcgatgg gcgagcgcac gccgctggcg
     1261 gtgatcgacg cgccggcttc cggacggatg gcggtgggcg aggcgatcac caacatcgcc
     1321 gctgcacgca ttgcgaaact cggcgacgtg aagttgtccg cgaactggat ggctgccgcc
     1381 ggccaccccg gagaagacgc ggctctgttc gacaccgtcg aggcggtggc gatggaactg
     1441 tgcccgcagt tgggcatcag catcccggtc ggcaaggact cgatgtccat gcgcacgtcg
     1501 tgggaagacg acggcaggaa aaaggaagtc gttgcgccgc tgtcgctgat cgtctccgcg
     1561 tttgcgccct gtctggacgc gcgcaagacg ctgacgccgc aactacagcc gcttgaccgc
     1621 gacagcatgc tggtgctgat cgacctcggc tcgggacgca atcgcctggg cggctcggtg
     1681 ctggcacagg tatacagcca aatcggcgat gaagcgccgg atctggactc gccacggaaa
     1741 ctgaaggcat tgttcgatac cgtccagcag cttagctctc ggggaagcat tctcgcgtac
     1801 cacgaccgtt ccgacggcgg gttgttcgcg acgctggcgg agatgatgtt tgcctcgcac
     1861 gtcgggatca cggcggacat aggcgcgctc ggtgaagatg cgttggctgc actcttcaat
     1921 gaggagctcg gcgcggtgct gcaagtgaag acttccgatt tcgatgccgt gaaaggcgcg
     1981 ttctcgcggg ccgggctggg cgcggagctt caccgtctcg gtatcatcaa tcgcgccggc
     2041 aggctgatca tcgtcgcaaa gggcaaggaa atatatagcg aatcgggaat cgccctgcaa
     2101 cgcgcgtggt cggaaaccac gtaccacatg caaaggctgc gcgacaaccc gcagtgtgcg
     2161 cagcaggaat atgaccgcat cctcgatgcc gcggatccgg ggctgcacgc gaaactgacc
     2221 tatgatccgg cgcaggacgt tgccgcgcct tacatcggcc gtggcgctcg cccgaagatg
     2281 gcgatcctgc gcgagcaggg ggtgaacggc cagatcgaaa tggcggcggc cttcgaccgc
     2341 gctgggttcg aaacgcgcga cgtgcacatg agcgacatca tcgccggccg cgcgtcgctg
     2401 gccggattca aaggtgtcgt ggcctgcggc ggattttcgt acggtgacgt gctcggcgcc
     2461 ggagagggct gggccaagtc catcctgttc aaccgccgcg cacgcgatga gttcgaggct
     2521 ttcttcaatc gcggcgactc cttcgcactg ggcgcgtgca acggctgcca gatgatgagc
     2581 aacctgcacg aaatcattcc cggcacggcg cactggccgc atttcgtacg caaccggtcc
     2641 gagcagttcg aagcgcgcgt ggtgatgctg gaagtgatga aaagtccgtc gctgttcttc
     2701 cgcgggatgg agggcagccg gatgccgatc gcggtcgcgc acggcgaggg ctacgccgag
     2761 ttcgcctctc ctgggcaact ggcagcaacc cgcgggctgg tggcgctgcg cttcgtcgac
     2821 aacgccggca ggccgacgga gcgctacccg ttcaatccga acggctcgcc gcaggggatc
     2881 acgggcctga ccaccgtgga cgggcgcttt accatcctga tgccgcacgc ggaacgggtg
     2941 ttccgcacgg cgcagaactc gtggcacccg gattcctggg gagaagacgg ggcgtggatg
     3001 cggatgttcc gcaatgcccg caaatgggtg gggtagcgca ccaaaaaaaa gcgctcacca
     3061 cggaggacgc ggaggacacg gaggcaaggc aagaacaaaa gagcattcga gagaaaaaac
     3121 ctggtatcgg tggtcacgtt ggcaccaagc ccgcttttaa ctcccacaag tttttttcct
     3181 cgtatttggt tttcctccgt gtcctccgcg tcctccgtgg tgagtgtttt ttttggtgcc
     3241 ct
//
LOCUS       DASQOP010000079        12828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1037058, whole genome shotgun sequence.
ACCESSION   DASQOP010000079 DASQOP010000000
VERSION     DASQOP010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12828
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037058"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..1114
                     /locus_tag="VE030_03040"
     CDS             158..1114
                     /locus_tag="VE030_03040"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZF22423.1"
                     /translation="MNKTVWAFGLAWLLTLSTPTQSQQPQAPADYPSSWAFPLKVEKR
                     LPSAGPEPMSLPGSPARYTQEQIDDLLNPPDWLPDQRPDPPAIVLKGHGDALACGACH
                     LLSGLGHPESTDLTGLTVAYMVQQMKDFRAGTRIDVPRMNKIAGAVAEDESQQAAAWF
                     ARLKPRLFTRVVEADIVPKTFVGDGRMRFVEPGGGTEPIGNRIITVPEDQTRARLRDP
                     NSGFIAYVPAGSIAKGKELAGTGGSGRTVPCAICHGPDLRGVGNVPRLTGAHPIYLAR
                     QLYRFRDGTRNGTEAALMKPAADRLTDEDIVNLSAYLGSLAP"
     gene            1320..1856
                     /locus_tag="VE030_03045"
     CDS             1320..1856
                     /locus_tag="VE030_03045"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZF22424.1"
                     /translation="MKNVLTGVLTALACFAAEAWGAEANELTASQSIRINAAPEAVWQ
                     VVGDYNGSPRWLPLVERSEIVFGKNNRVGAIRLITRRNGTKVTERLLDHDQQNMRMAY
                     TYVDGAVMASDYFPVISVKDAGDGTTIVEWSAHFRRLAYSIDPPPPGQDDKSMTDFYN
                     GIYKSGLESLKRVVESGQ"
     gene            1891..3213
                     /locus_tag="VE030_03050"
     CDS             1891..3213
                     /locus_tag="VE030_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22425.1"
                     /translation="MTNMARRTHLTGLLGLASLACLPPGSRAASDAPVPPHGIGRRIR
                     HLSYSDQGGRPDGVQVMVNRRHVYVGHMFSDGVTILDAAEPRGLRPVGFFTAGVNTRT
                     HHLQVADDLLLLANGANIVAMQSYDNMRGYFENTLADSITDRKKFRSGLSIHDISRPT
                     EMKEIAFLEMPGFGINRLWWPGGRYAFVSAHFDGYTDHILCIVDLQNITKPEIASRWW
                     LPGMHRAGGETSALPKGRRVALHHMITAGDRGYAAWRDGGFTIHDISDPTKARLLSHI
                     NWSPPFPGGTHTPLPLPGRGLAVVADEANAEKCQKGTFHTFVVDVRAPENPVPIATLP
                     TPADRDYCSLGVFGPHNLHENRPGSFRSEEIIFATYNNAGVRVFDIKDAFAPKEIASW
                     IAPAPKRLIDPRPNISLAAKTCDAYVTPEGLMFVSDWNAGMHVLQYEG"
     gene            3310..4674
                     /locus_tag="VE030_03055"
     CDS             3310..4674
                     /locus_tag="VE030_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HZF22426.1"
                     /translation="MAAQKILDLIGNTPLVEVTRMDAAKCRLFLKMESQNPGGSIKDR
                     IGLSMIGAAERDGKLSPGGTIIEATAGNTGLGLALVAALKDYRLILVVPDKMSREKIF
                     HLKAMGAEVMLTRSDVGKGHPEYYQDMAERLARETPGSFFVNQFGNPANPHAHETTTG
                     PEIWEQMDHKVDAVVCGVGSAGTMTGLSRYFAKVSPDTAMVLADPVGSVLADYVHTGK
                     VGVAGSWIVEGIGEDFIPPIADFSRVSKAYSISDEEACTTVRDLLKKEGILAGSSSGT
                     LLAAALRYCREQTQPRRVVTFVCDSGNKYLSKVFNDYWLMDQGFLKREQYGDLRDLIT
                     RRHDEHATVTVGPDDKLMTAYARMKLYDVSQLPVLEDGKVVGIVDEWDLLTATQENPA
                     RFRDPVRSAMTQRIETVGLKTPLTKLTEAFNKGHVAIVVDNGKFYGLITRMDVLNHLR
                     NRAH"
     gene            4891..6060
                     /locus_tag="VE030_03060"
     CDS             4891..6060
                     /locus_tag="VE030_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HZF22427.1"
                     /translation="MKNNQDKQGFATRAIHAGQSPEPTTGAVMTPIFATSTFKQSSPG
                     VHTGYEYARTQNPTRHAFERCIADLEGGMQAFAFASGLAASATVLELLDAASHMVAVD
                     DMYGGSWRLFERVRKRSMGLKVSYSDPNIAGSLEKALQPDTKLIWVETPSNPLLKVTD
                     LAQIAKIGRERGILTVVDNTFASPFVQRPLDLGIDLVVHSVTKYINGHSDMVGGIVVV
                     RERKDLVEKLEFMQNAIGSILDPFSSFLALRGVKTLLLRMTQHCGNAMHLAAWLKSHP
                     KVERVIYPGLPSHPQHLLAAKQMNGFGAMIAVYLKTDMDGVRRALEACKVFTLAESLG
                     GVESLIGHPVTMSHGSLPVELRAKLGITDNLVRLSVGIEDINDLIADLDQALTRI"
     gene            6060..6566
                     /locus_tag="VE030_03065"
     CDS             6060..6566
                     /locus_tag="VE030_03065"
                     /inference="COORDINATES: protein motif:HMM:NF014639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HZF22428.1"
                     /translation="MMNARRIYTAIFLVCCGLIGFGLYLQHAQGLEPCPMCILQRYAF
                     VAIGVIALAAALHNPGITGRWGYSALLVIFAGLGGGVAIRHAWLEHNPPKVFDCGADL
                     GYMMESLPLADALPMIFRGTGDCTKVLWRFLGLSIAEWALIWFAIFLVGAIVAAAMKP
                     KRLRMFGP"
     gene            complement(6576..7232)
                     /locus_tag="VE030_03070"
     CDS             complement(6576..7232)
                     /locus_tag="VE030_03070"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZF22429.1"
                     /translation="MTSPSRIALRFVGVLAVLGLLAAFSSVVLSAPSGVPDQEHALSG
                     ESLAAALKRGGYVIYFRHADTGPAYLEQGVDLKRCETQRNLNDNGREEAKLIGAQFRK
                     LNIPVGEVLASEFCRCRETADLAFGRHKLEPLLTGVSRSAEAAPRRERAIAALKKMLA
                     ATPPPGTNTVLVSHGFNLWDAEGFHLGTQGEAAIYKPDGQGGYALVARLLPRDWGELP
                     "
     gene            complement(7235..8134)
                     /locus_tag="VE030_03075"
     CDS             complement(7235..8134)
                     /locus_tag="VE030_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZF22430.1"
                     /translation="MKSPRLDAIKSLAFRQPRQFRLPVRGEVIVNNERRYFIGEPIGK
                     GGFGMVYECSDDWGNALVAKILLPHDKSYREVRKIWQDELQKLLVMRHPNITFVYDAF
                     EYQDTFYLIIERCSFPLTNLIARRGLDADRWIPYVGSDILQALDYIHAAGYVHKDLHP
                     GNVFVTETRRKRRLLGEPVWSFKVGDLGLTRLESDIRVFQTLLADWMRPPEAYNPEEF
                     GAVGRHVDIYQTGLLLLALLLKEIPYFTQEEILGGIPQALATKHGSKYGAVIAKALRR
                     HVEWRTQTALEFWHELSSVTSEG"
     gene            complement(8154..8651)
                     /locus_tag="VE030_03080"
     CDS             complement(8154..8651)
                     /locus_tag="VE030_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HZF22431.1"
                     /translation="MSEKAGWKGRFYEDFEVGDLYRHPLGRTVTQTDNIWFTLLTQNT
                     APIHFDAHYASQTEFGKPLVDSTFTVALVTGQSVSDVSQNAFANLGWDEIRLPNPVFE
                     GDTLYSQSEVLEKRESRSRPSVGIVAVKTTGFNQDGKIVITFKRTVMVYRRGQAPVIP
                     RPILK"
     gene            complement(8822..10000)
                     /locus_tag="VE030_03085"
     CDS             complement(8822..10000)
                     /locus_tag="VE030_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HZF22432.1"
                     /translation="MLPLKGITVVSLEQAVAAPFTTRQLADLGARVIKIERPQVGDFA
                     RGYDKTVLGQSSHFVWLNRSKESLTLDVKHPDAAGILDRLLKKADVFVQNLVPGAAAR
                     LGLSSEDLLDKNPRIVACNISGYGPDGPYSLKKAYDLLIQCESGVVSVTGTPETPAKS
                     GISIADIAAGMYGYTGILTGLYQLERTGQGTAFDVSMFEALGEWMGYPAYFAGYGGTA
                     PARTGAAHATIHPYGPFPAGDGKTVFFGIQNEREWANFCAIALEQPRLADDPRFDNNS
                     HRVANRRALTDIIVSAFSKLTAEQAMEGLDKAQIANARLNTMQEFWDHPQLQARDRWR
                     EVDSPQGTIKALVPPVTMQGVEPRMDPIPAVGQHTLAILRELGFDAGFIAKLENEKAI
                     "
     gene            complement(10198..10860)
                     /gene="nthA"
                     /locus_tag="VE030_03090"
     CDS             complement(10198..10860)
                     /gene="nthA"
                     /locus_tag="VE030_03090"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HZF22433.1"
                     /translation="MPHDHDHPHDHDHRHGHTRPHRHPPRPDQDDTITQWRAMEIAVR
                     ELLIEKGILSAEEVRRQIEDMDKRTALQGARVVARAWTDPEFRKLLLSDGNAACEALG
                     MEGGPYKLVVVENTDQVHNVIVCTLCSCYPRWLLGLPPDWYKSRNYRSRAINEPREVL
                     KEFGTTIARDVTVRVHDSTADMRYLVLPKRPGGTEGWSEEKLAALVTRDSMIGVAFAQ
                     NP"
     gene            complement(10862..11164)
                     /locus_tag="VE030_03095"
     CDS             complement(10862..11164)
                     /locus_tag="VE030_03095"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3-like domain-containing protein"
                     /protein_id="HZF22434.1"
                     /translation="MTAAERISPQFRTGDRVAVRAAYPIGHVRTPYYIRGKRGVIERL
                     CGAYGNPEELAYARPGLPKQPLYRVRFFQREVWPTYEGNPGDTVDIEIFQHWLEPA"
     gene            complement(11151..11465)
                     /locus_tag="VE030_03100"
     CDS             complement(11151..11465)
                     /locus_tag="VE030_03100"
                     /inference="COORDINATES: protein motif:HMM:NF045268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22435.1"
                     /translation="MNYRGIHDMGGLPAGTVQPTEHDYALWEKRVDALMVLLTKKGLM
                     TVDELRRNIEGLGQDAYDKMSYYERWVYSIAQTLIQRGVISVDELGRKMVEAQTGTDD
                     GR"
     gene            complement(11569..>12828)
                     /locus_tag="VE030_03105"
     CDS             complement(11569..>12828)
                     /locus_tag="VE030_03105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZF22436.1"
                     /translation="NSTRDSKPKRAARLPRGIEEMAIQRRESFVLFYVIAFVTVGVAA
                     GAVWYYWSSKQSHLVQDAKARAVELDAGLTVVVATSAKGPTARRITLVGEAAAHKSTT
                     LYSKVGGYLTRIAVDTGDRVKSGQFIAEVETPELESEYRSAVANLENKQRVVQRTRNL
                     AQKGFFSQQALENAETEVTLELGHVQELRTRLGYKTLRAPFSGVVTARHVDPGALVTN
                     AANNQTSSQPVVTIADTSRLRVTVYVEQVDAPFVRPGIEVEIVDAANPERRNRGRISR
                     IGGELDSRTRTLLAEVDFDNGKGEFIAGSYVNVTLLIPAVSYVEVPAPALVVRDKKNY
                     VAVVGGNNHIKLVPIEVAGTDGRVIRILNGLGEGVRVALNLPNTVPDDAKVNPSVPPG
                     TPAPPPPAASGQPAAAQPAPVGSAQPK"
BASE COUNT         2405 a         4027 c         3940 g         2456 t
ORIGIN      
        1 cctcaccgag tccgacctct cgcactcgca ggaaatcctc gacgtggttt acagcatcga
       61 ccgcggagag gtttcgatcg ctacatcgcc ggcttagagc ccgcgaatcc gggctgcggg
      121 ctcgtcattg gcctcagcca attgcaggga ggttctcgtg aacaaaacgg tatgggcgtt
      181 cggtctcgcg tggctgctaa cgctgtcaac gccaacgcag tcgcaacagc cgcaggcgcc
      241 agccgactat ccgtcgtcgt gggcattccc cttgaaggtg gagaaacggc ttccgtcggc
      301 agggccggag cccatgtcgc tgcccggtag cccggcaaga tacacacagg aacagatcga
      361 cgatctgctg aacccgcccg actggttgcc cgaccagcgt ccggacccgc ccgcgatcgt
      421 cctcaagggg cacggcgacg cattggcatg cggagcgtgt cacctcctgt ccggcctcgg
      481 ccacccggag tccaccgacc tcacgggcct gaccgtggcg tacatggttc aacagatgaa
      541 agatttcagg gccggcacga gaatcgacgt gccaaggatg aacaagattg cgggggccgt
      601 ggccgaggac gagtcacaac aggcggccgc gtggtttgcc aggctcaagc cccgcctgtt
      661 cacccgggtc gtcgaagccg acatcgtgcc taaaacgttc gtcggcgacg gtcgtatgcg
      721 ctttgttgaa ccgggtggcg gcaccgagcc gatcggcaac cgcatcatta ccgtgcccga
      781 ggaccagacg cgagcgcgcc tgcgcgatcc gaattcggga ttcatcgcct acgtccccgc
      841 cgggagcatc gcgaaaggca aggaacttgc ggggaccggc ggcagtggca gaacggttcc
      901 gtgcgcgatt tgtcacggtc cggacctgag aggggtgggc aatgtgccga gactcaccgg
      961 cgctcatccg atctacctcg cgcgccagct ttaccgcttc agggatggga cgcgaaacgg
     1021 caccgaggcc gcactgatga aacctgccgc cgacaggttg actgacgaag atattgtcaa
     1081 cctctctgcg tacctgggat ctcttgcgcc ttaggccacc agcgccagtt tccaggctgc
     1141 agactgactc gaaacctcgc tctgtggcct gttaccaacc gtggaacggt tgaattcctc
     1201 tcctgtgagc gtctccgagg tttaggatac ggtcgcgttg ctcgaaagcc tcgcgacgtg
     1261 cctgcaagaa atcaagcata gaggcctcgc atcaccagtg aatgggagga cggaaagcca
     1321 tgaagaacgt attgacaggt gtactgacgg cgctcgcttg ttttgcggcc gaagcctggg
     1381 gcgcggaggc gaacgaactg accgcaagcc agtcgatccg gatcaacgcg gcgcctgagg
     1441 cagtgtggca agtggtcggg gactacaacg gatctccgcg ctggctgccg ctcgtcgagc
     1501 gcagtgagat cgtgttcggc aagaacaacc gggtcggcgc gatccgcctg atcacgcgac
     1561 gaaacggcac gaaggtcacg gagcggctgc tggaccatga ccagcagaat atgcgcatgg
     1621 cctataccta cgtggatggg gcggtcatgg cgagcgacta ctttccggtc ataagcgtca
     1681 aggacgccgg cgacgggaca accatcgtgg agtggagcgc gcacttcagg cgcctggcct
     1741 actcgatcga cccgccgccg cccggacagg atgacaagtc gatgacggac ttctacaacg
     1801 ggatctacaa aagcgggctg gagagcttga agcgcgtcgt ggagagcgga cagtaattcc
     1861 gcgagggaag cggctcgccg cagtaccagc atgaccaaca tggctcgccg cacgcacttg
     1921 acgggtctgc tcggcctggc ttcgttggcg tgcctgccgc ccggatcacg cgccgcttcg
     1981 gacgcgcccg ttccgccgca cggcatcgga cgccgcatcc ggcacctgtc ctacagcgat
     2041 cagggcggtc gccccgacgg cgtacaggtc atggtcaacc gccgccatgt ctatgtcggc
     2101 cacatgttca gcgacggggt aaccattctc gacgccgcgg agccgcgtgg gcttaggccc
     2161 gtcggatttt tcaccgcggg cgtcaacacc cggacgcatc acttgcaagt ggcggacgat
     2221 ctactgctgc ttgccaacgg cgcgaacatc gtcgcaatgc agtcctacga caacatgcgt
     2281 ggttacttcg agaacacgct cgccgacagc atcaccgacc gcaagaagtt ccgctcgggc
     2341 ctgagcatcc acgacatctc ccgccccacg gagatgaagg agatcgcgtt tctcgaaatg
     2401 cccggcttcg gtatcaaccg gctgtggtgg ccgggcgggc gctacgcctt tgtatccgcg
     2461 cacttcgacg gctacaccga ccacatcctg tgcatcgtcg atctgcagaa catcaccaag
     2521 ccggagatcg catcgcgctg gtggctgccc ggcatgcatc gggcgggtgg cgagacatcg
     2581 gcgctgccca agggccgccg cgtcgctctg catcacatga tcaccgcggg cgatcgcggc
     2641 tatgccgcct ggcgcgatgg cggcttcacc atccacgata tcagcgaccc gaccaaagcc
     2701 aggctgctct cgcacatcaa ttggtcaccg ccttttcccg gcggtaccca cacgccacta
     2761 ccgttgccgg gacgcggcct cgcggtcgtg gcggacgaag cgaacgccga aaaatgccag
     2821 aagggaacgt tccacacctt cgtcgtggac gtgcgtgcgc cggagaaccc ggttccgatt
     2881 gccacgttgc ccacaccggc agaccgcgac tattgctcgc tgggcgtgtt cggtccgcac
     2941 aacctgcacg agaaccggcc ggggtccttc cgcagcgagg aaatcatttt cgcaacctac
     3001 aacaatgccg gggtgcgcgt gttcgatatc aaggacgcgt tcgccccgaa ggagatcgcc
     3061 tcctggatag cgccggctcc caaaaggctg atcgacccgc ggccgaacat ctcgcttgca
     3121 gcaaagacct gcgacgccta cgtcacgcct gaggggttga tgttcgtgag cgactggaat
     3181 gcgggcatgc acgtgctgca gtacgaaggc tgaacggttt cagacggcaa gcccacgcac
     3241 ggaagagtat ggtcccgcgt tacactcgcg cttcatcggc gctcatcgtg gctctggagg
     3301 gaccccgccg tggccgcaca gaaaatactg gacctgatcg gaaacacgcc gctggtcgaa
     3361 gtgacccgca tggacgcggc caaatgccgg ctgttcctga agatggaaag ccagaatccc
     3421 gggggctcga tcaaggaccg catcggcctg tcgatgatcg gcgccgcgga gagggacggc
     3481 aaactcagtc ccggcggcac catcatcgag gccaccgccg gcaacaccgg tctggggctg
     3541 gcgctggtcg ccgcgctcaa ggattatcgc ctgatcctgg tggtgccgga caagatgagc
     3601 cgcgaaaaga ttttccatct caaagcgatg ggcgcagagg tcatgctcac ccgctccgat
     3661 gtcggcaagg gccaccccga gtactaccag gacatggccg agcgcctggc ccgggaaacg
     3721 cccggttcgt ttttcgtgaa ccagttcggc aatcccgcga atccgcatgc gcacgaaacc
     3781 accaccggac ccgagatctg ggaacagatg gatcacaaag tggacgccgt tgtgtgcggc
     3841 gtcggctcgg ccggcaccat gacggggctg tcgcgttatt tcgccaaagt ctcacccgac
     3901 accgcgatgg tgcttgccga tccggtgggg tcggtgctcg ccgactatgt ccacaccggc
     3961 aaggtcggcg tggcgggcag ctggatcgtc gagggtatcg gcgaggattt catcccgccg
     4021 atcgccgatt tctcccgcgt cagcaaggcc tactcgatca gcgatgaaga ggcctgcacg
     4081 accgtacgcg acctgctgaa gaaagaaggc atcctcgccg gttcttcatc gggcacgctg
     4141 ctcgccgctg cgctgcgtta ctgccgcgaa cagacccagc ccaggcgtgt cgtgactttc
     4201 gtctgcgaca gcggcaacaa gtatctgtcg aaagtgttca acgattactg gctgatggac
     4261 cagggcttcc tcaagcgcga gcagtacggc gacctgcgcg atctgatcac gcgccgccac
     4321 gacgagcacg ccaccgtgac cgtcggtccg gacgacaagc tgatgaccgc gtatgcgcgc
     4381 atgaagctct acgatgtctc gcagttgccg gtgctcgaag acggcaaagt agtgggcatc
     4441 gtcgacgagt gggatctgct caccgcgact caggaaaacc cggcacgctt tcgcgacccg
     4501 gtgcgctcgg cgatgaccca gcgcatcgag accgtaggcc tgaagacgcc gttgacgaaa
     4561 ttgacggagg cgttcaacaa gggtcacgtc gctattgtcg tggacaacgg caagttctac
     4621 ggcctgatca cccgcatgga tgttctgaat catctgcgca atcgcgccca ctgattacta
     4681 ctgcatattt tcaccgcaaa gacgcaaaga acgcagagaa taccggtgac aaaaaacaaa
     4741 ctggggttga gaccccacca cggtggcacg ccgtctttaa cccagtctgt ttttccgact
     4801 tgattattct tttccttcgc gttctttgcg tctttgcggt gaaacggctt tgtggtgaaa
     4861 agaggttgtc ttaacaggta agattcggga atgaagaaca atcaggacaa gcaaggcttc
     4921 gccacccgcg ccatccatgc cggccaatct cccgagccca ctaccggcgc ggtgatgaca
     4981 ccgatttttg ccacctccac cttcaagcag agttctccgg gagtgcacac cggctacgag
     5041 tacgcgcgca cccagaatcc cacacgccac gccttcgagc gctgcatcgc ggacctggag
     5101 ggcggcatgc aggcctttgc attcgcttcg ggacttgccg ccagcgccac cgtgctggaa
     5161 ttgctggatg cggccagcca catggtcgcg gtcgatgaca tgtacggcgg cagctggcgc
     5221 ctgttcgagc gcgtgcgcaa gcgctcgatg gggctcaagg tgagttattc cgaccccaac
     5281 atcgccggct ccctggaaaa ggcgttgcag ccggacacga aattgatctg ggtcgagacg
     5341 ccttccaacc cgctgctcaa ggtcaccgac cttgcgcaaa tcgcgaagat cggcagggag
     5401 cgcggaattc ttaccgtcgt ggacaatacc ttcgcttcgc ctttcgtgca gcggccgctc
     5461 gatcttggca tcgacctcgt ggtccattcg gtgaccaagt acatcaacgg acactcggac
     5521 atggtcggcg gcatcgtggt cgtcagagaa cgcaaggacc tggtggagaa actggagttc
     5581 atgcagaacg ccatcggttc catcctcgac ccgttctcca gttttcttgc gctgcgcggg
     5641 gtcaagacgc tgctgctgcg catgacgcag cactgcggca atgccatgca tctggccgcg
     5701 tggctgaagt cgcatccgaa agtggagcgg gtgatttatc ccggactgcc cagccacccc
     5761 caacacctgt tggcggcgaa acagatgaac ggtttcggcg ccatgatcgc ggtctatctc
     5821 aagactgaca tggatggcgt gcgccgggcg ctggaagcct gcaaagtgtt cacgctcgca
     5881 gaaagcctgg gtggcgtaga aagcctgatc gggcatccgg tcaccatgag ccacggctcg
     5941 ctgccggtgg aacttcgcgc caagctcggt atcaccgaca acctggtgcg gctgtcggtc
     6001 ggcatcgagg acatcaacga cctgatcgcg gacctcgatc aggcactgac aaggatctga
     6061 tgatgaatgc gcgtcggatt tacacggcga ttttcctggt ctgctgcgga ttgatcggct
     6121 tcgggttgta tctccagcac gcacagggac tcgaaccttg cccgatgtgc atcctgcagc
     6181 gctatgcctt cgtcgccatc ggcgtgatcg cgctggccgc agccctccac aatccgggaa
     6241 taaccggccg gtggggttac agcgcgctgc tggtgatttt tgccggtctc ggcggcggtg
     6301 tcgccattcg ccacgcgtgg ctcgagcaca atccacccaa ggtcttcgac tgcggcgcag
     6361 acctcggcta catgatggag tccctgccgc tggccgatgc gctgcccatg atcttccgcg
     6421 gtaccggcga ctgcaccaag gtgctgtggc ggttcctcgg actttcgatt gcggagtggg
     6481 cgttgatctg gttcgcgata ttcctggtcg gcgcgatcgt tgccgcggcg atgaaaccga
     6541 aacggctgag gatgttcggt ccctgaggca agggactagg gaagctctcc ccagtcgcgc
     6601 ggtagcaaac gcgccaccag cgcgtagccg ccttggccgt cgggcttata gatcgcggcc
     6661 tcgccctggg tgccaagatg aaagccttcc gcgtcccaca gattgaagcc gtgagagacc
     6721 agcacggtat tcgtgccggg cggcggcgtt gctgcaagca tcttcttcag ggcagcgatc
     6781 gcccgttccc ggcgcggcgc cgcctcggcg gagcggctta cgccggtaag caacggctcg
     6841 agcttgtggc gtccgaacgc gaggtccgca gtttcccggc agcggcaaaa ctcgctcgcc
     6901 agcacttcgc cgaccgggat gttcaacttc ctgaactgcg cgccgatcag cttcgcttcc
     6961 tcgcggccgt tgtcgttgag attgcgctgg gtctcgcagc gtttcagatc cacgccttgc
     7021 tcgaggtagg ccggtccggt atcggcatgg cggaaataga tcacgtagcc cccgcgcttc
     7081 aatgcggccg cgagcgattc gccgctcagc gcgtgttcct ggtccggcac accggacggt
     7141 gccgaaagaa ctaccgacga gaacgctgcc agcagtccca ggaccgccag aacgccaacg
     7201 aatcgcaacg cgatgcgcga ggggcttgtc accctcaacc ctcactcgtc acgctcgaca
     7261 actcgtgcca gaactcgagc gcggtctggg tgcgccactc gacatgtcgg cgcagcgcct
     7321 tcgcgatcac cgcaccgtac tttgagccgt gcttggtagc caacgcctgc ggaatgccgc
     7381 cgagaatttc ctcctgggtg aagtaaggga tctccttcaa caacagcgcc agcagcaaca
     7441 atccggtctg atagatgtcc acgtgacgcc cgactgcgcc gaactcttcg ggattgtatg
     7501 cttcgggcgg ccgcatccag tcggccagca gcgtctggaa tacccggatg tcggattcca
     7561 gccgggtcag gccgagatcg ccgaccttga acgaccacac cggctccccc agcagcctgc
     7621 gcttgcgccg cgtctcggtg acgaaaacgt ttcccggatg caggtccttg tggacgtagc
     7681 cggcggcgtg gatgtagtcg agcgcctgga ggatgtcgct cccgacatag ggaatccagc
     7741 gatccgcgtc caggccgcgg cgggcgatca ggtttgtgag cgggaagctg caacgttcga
     7801 tgatgagata gaaggtgtcc tggtactcga acgcgtcgta gacgaacgtg atgttcggat
     7861 ggcgcatgac cagcagcttt tgcaactcgt cctgccagat cttgcgcacc tcccggtagg
     7921 acttgtcgtg cggcagcagg atcttggcaa ccagtgcatt tccccagtcg tcgctgcact
     7981 cgtacaccat gccgaatccg cccttgccta tcggctcgcc gatgaagtag cgccgctcat
     8041 tgttgacaat cacctcgccc ctgaccggca gccggaactg ccgcggctgc cggaacgcca
     8101 ggctcttgat ggcgtcgagg cgtggcgatt tcatgggatg tgtactgcca tggtcacttc
     8161 agtatcggtc gcggaatgac cggcgcctgc ccgcgcctgt aaaccatcac ggtgcgtttg
     8221 aaagtgatga cgatcttacc gtcctgattg aagccagtcg ttttcacggc cacgatgccg
     8281 acgctgggtc gcgaccggga ttcgcgtttc tccagcacct cggactggga gtagagggtg
     8341 tcgccttcga aaaccggatt gggcaggcgg atttcatccc agcccaggtt ggcaaaggca
     8401 ttctgggata catcggacac gctctgtccg gtgaccaagg caacagtgaa cgtcgaatcc
     8461 accagtggct tcccgaactc cgtttgtgag gcgtaatgtg cgtcgaaatg aatcggcgcc
     8521 gtattctggg tgagcagcgt gaaccagatg ttgtcggtct gcgtcacggt gcggcccagc
     8581 ggatgtcgat acaagtcgcc gacctcgaaa tcctcgtaga acctcccctt ccagcctgct
     8641 ttctcgctca tgacgaatcc taaatcagtt agccacagag gacacagaga gcacagaaaa
     8701 aagccggata gacgaaaaac ggctacccga aatgaccatg gaaaaatgaa caagatcgaa
     8761 cgcgatactt tttctgttcg gagtctattg tagttctctg tggcctctgt gacctctgtg
     8821 gctaaattgc tttttcgttt tccagcttcg caatgaagcc ggcgtcgaaa cccagttctc
     8881 gcaggatcgc aagcgtgtgc tggccgacgg cggggatcgg atccatgcgc ggctccaccc
     8941 cttgcatggt caccggtggg accagcgcct tgatcgtgcc ctgcggcgaa tccacttcgc
     9001 gccagcggtc gcgggcctgc aactgtggat ggtcccagaa ttcctgcatg gtattcaggc
     9061 gcgcattggc gatctgcgcc ttgtccagcc cctccatcgc ttgctccgcg gtcaatttcg
     9121 agaatgcgga gacaatgatg tcggtgagcg cccgccggtt ggcaacgcga tggctgttgt
     9181 tgtcgaagcg cgggtcgtct gccagccgcg gctgctccag tgcgatcgcg cagaaattcg
     9241 cccattcccg ttcattctgg atgccgaaga acacggtctt gccgtcgccc gccggaaacg
     9301 gcccgtaagg atgaatggtg gcgtgcgcgg cgccggtgcg cgcaggcgcg gtgccgccgt
     9361 agccggcgaa gtaggcaggg taccccatcc attcgcccag cgcctcgaac atcgagacgt
     9421 cgaacgccgt gccctggccg gttctctcga gctggtaaag cccggtaagg atgccggtgt
     9481 aaccgtacat tcctgccgcg atgtcggcga tcgagatgcc cgacttcgcc ggcgtctcgg
     9541 gcgtgcccgt cacggagact actcccgact cgcactggat cagcagatcg taggctttct
     9601 tcaggctgta gggaccgtcg ggaccgtagc cggaaatatt gcaggcgacg atgcgcgggt
     9661 tcttgtccag cagatcttcg gaggatagcc ccaatcgcgc cgccgcgccg ggcaccaggt
     9721 tttgcacgaa cacatcggct ttcttcaaca gccggtcgag gattcccgca gcgtccggat
     9781 gcttcacgtc cagcgtgaga ctttccttgg agcggttgag ccagacgaaa tgcgaggact
     9841 ggccgagcac ggtcttgtcg tagccgcggg cgaaatcgcc gacctgcggg cgttcgatct
     9901 tgatcaccct tgcgccgaga tctgcaagct gccgcgtggt gaacggtgcc gccacggctt
     9961 gctcaagcga tacgacggtt atgcctttca gtggcagcat caacaaaccc taactgcaga
    10021 ggaaacgcaa aggtaaacaa aataatgtga tggaaaagca agaaagaaat ggatagctgc
    10081 gacaatggcg attcatcgat tgggcaacga cggctctgaa ttcatcccaa cttttttctt
    10141 gccggcttct tttggttttc tctgcgttcc ctttgcgcct ctgcggtaaa agtgctttca
    10201 aggattctgc gcgaaggcga cgccgatcat cgagtcgcgg gtcaccagcg cggctagttt
    10261 ctcctcagac cagccctcgg tgccccccgg acgcttcggc agcaccagat aacgcatgtc
    10321 ggcggtggaa tcgtggacgc gcaccgtcac gtccctggca atcgtggtgc cgaattcttt
    10381 gaggacctcg cgcggttcat tgatcgcccg cgaccggtag ttgcggctct tgtaccagtc
    10441 cggcggcagc ccaagcagcc agcgcggata gcaggagcac agcgtgcata cgatgacgtt
    10501 gtgcacctgg tcggtgttct ccacgaccac cagtttgtac ggccctcctt ccatgccgag
    10561 cgcctcgcag gccgcgttgc cgtcgctcag cagaagcttc cgaaattccg ggtcggtcca
    10621 ggccctggcc accaccctgg cgccctgcag cgccgtgcgc ttgtccatgt cctcgatctg
    10681 acggcgcact tcctcggcgc tgaggatgcc cttctcgatc agcaactcgc gcaccgcgat
    10741 ctccatcgcc cgccattgag tgatggtgtc gtcctggtcc gggcgcggcg gatgccggtg
    10801 tggccgggtg tgaccgtgcc gatggtcgtg gtcgtgcggg tgatcgtgat cgtgcggcat
    10861 gtcaggccgg ctccagccag tgttggaaga tttcgatatc gacggtatcg ccgggattgc
    10921 cctcgtacgt gggccagacc tcacgctgaa aaaaacgcac gcggtatagc ggctgcttcg
    10981 gcagacccgg gcgggcgtaa gcgagttcct cgggattgcc gtatgcacca cacaggcgct
    11041 cgatcacccc gcgtttgccg cggatgtagt agggggtgcg cacgtggccg atcggatagg
    11101 cggcccgcac cgcgacgcgg tccccggtgc ggaattgtgg cgatatcctt tcagcggccg
    11161 tcatcggtgc cggtctgcgc ctcgaccatc ttgcggccga gttcatcgac gctgatgaca
    11221 ccgcgctgga tcagcgtttg cgcaatcgag tagacccagc gctcgtaata gctcatcttg
    11281 tcgtaagcat cctgccccag accctcgata ttgcgccgga gctcgtccac cgtcatcagt
    11341 ccctttttgg tcaggagcac catcaacgcg tccacgcgct tttcccagag cgcgtagtca
    11401 tgctcggtcg gttggacggt tcccgccggg agaccgccca tgtcatggat gccgcgatag
    11461 ttcatgttgg cccgcgtcag atcggtgtat accggcgccc gcgtttgccg cgtgcggcgt
    11521 gtcccgcttt caggttgcgc tcgaggactg aattatagga tgcctgcgtt atttcggctg
    11581 cgcgcttcca accggggccg gctgggccgc agccggctga ccggaagctg ctggcggcgg
    11641 tggcgccggg gtgccgggcg gaaccgacgg gttgaccttg gcgtcgtcgg gaaccgtgtt
    11701 gggcaggttc agcgcaaccc gaacgccttc gccgagcccg ttcaggatcc tgatcacgcg
    11761 gccgtcggtg ccggcgacct cgatcggcac cagcttgata tggttgttgc caccgacgac
    11821 ggcgacatag ttcttcttgt cgcgcaccac cagcgccggc gccggcactt cgacgtaact
    11881 gacggcggga atcagcagcg tgacgttgac gtaactgccg gcaatgaatt cgcccttgcc
    11941 gttgtcgaaa tccacttccg ccagcagcgt gcgtgtgcgc gagtcgagtt cgccgccgat
    12001 gcggctgatc ctgcccctgt tgcggcgttc gggattcgcg gcatccacga tctcgacttc
    12061 gatcccgggt ctgacgaacg gtgcgtccac ctgctcgacg tacacggtga ctcgcagacg
    12121 cgatgtgtcg gcgatggtga ccaccggttg cgatgacgtc tggttgttgg cggcgttggt
    12181 caccagtgca cccggatcga catgccgtgc agtcaccacg ccggagaatg gcgcgcgcaa
    12241 cgtcttgtag cccagcctgg tgcgcagttc ctggacgtga ccgagctcga gcgtgacctc
    12301 cgtctctgcg ttttcgagcg cctgctggga aaaaaagccc ttctgcgcaa gattacgggt
    12361 gcgctgcacg acccgctgct tgttctcgag attcgccact gcgctgcggt attcggactc
    12421 gagttccggt gtctccacct cggcaatgaa ctggccgctt tttacgcggt cgccggtatc
    12481 aaccgcgatc ctggtgagat agcccccgac cttcgaatac agtgtcgtcg atttgtgcgc
    12541 cgccgcctcg cccaccagcg tgatccggcg cgcagtcggc ccctttgccg aagtcgccac
    12601 cacgaccgtc agtccggcgt cgagctcgac cgcccgggcc ttcgcatcct ggaccagatg
    12661 gctctgcttg gagctccagt aataccacac ggcaccggcg gcaacgccga cagtgacaaa
    12721 cgcgatgacg tagaagagaa caaacgattc gcgacgctgg attgccattt cttcgattcc
    12781 tctaggcagt ctcgccgcgc gcttcggctt cgaatcgcgc gtcgagtt
//
LOCUS       DASQOP010000080        14479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1238710, whole genome shotgun sequence.
ACCESSION   DASQOP010000080 DASQOP010000000
VERSION     DASQOP010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14479
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238710"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..321
                     /locus_tag="VE030_03110"
     CDS             64..321
                     /locus_tag="VE030_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HZF22437.1"
                     /translation="MRVLIPSALRSYTERSEAEAGGATLAAVLADLDRQYPGIRFRMI
                     DEQDRIRPHIRIFVNGEQVRDPSQLLRATDEVVIVQALSGG"
     gene            371..1312
                     /locus_tag="VE030_03115"
     CDS             371..1312
                     /locus_tag="VE030_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZF22438.1"
                     /translation="MPPVVSISNLAKTYASGLQALKKVDLEVRKGEIFALLGPNGAGK
                     TTLISIICGIVTPTSGTVLVDGRDILRDYRECRSKIGLVPQELQTDMFETVWNTVNFS
                     RGLFGRAPDPAHLAKVLRDLSLWDKRNDRIMTLSGGMKRRVLIAKALAHEPEILFLDE
                     PTAGVDVELRRDMWTLVQRLRAGGVTIILTTHYIDEAEEMADRIGVISKGELIVVEEK
                     TRLMKKLGKKQLTLHLLEPMTAIPAELGTWSLVLKTEGTVLEYNFDAAEEGTGIPALL
                     RRLSELGIGFKDLQTEQSSLEDIFVSLVAKNSEGGRA"
     gene            1309..2097
                     /locus_tag="VE030_03120"
     CDS             1309..2097
                     /locus_tag="VE030_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006766403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZF22439.1"
                     /translation="MSTAAKIPSFNRHGVLAIYRFEMARFKRTVWQSLVTPVITTSLY
                     FVVFGAAIGSRISEVGGVPYGSFIVPGLIMLSLFTESLNNASFGIYLPRFTGTIYEVL
                     SAPVSALEIVLAYVGAAATKSVMLGLVILATAMLFVPMRIEHPLWMIAFLVLTAATFS
                     LFGFIIGIWANGFEQLQFIPMLIITPLTFLGGAFYSIDILPPAWRAVSLFNPIVYLVS
                     GFRWSFYSTSDVGVGVSLAATLAFFVLCLAVVVWVFRTGYRLKS"
     gene            2318..3460
                     /locus_tag="VE030_03125"
     CDS             2318..3460
                     /locus_tag="VE030_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZF22440.1"
                     /translation="MMRILVQVFCRPSLLLALLSGIAAAQQPAGEPIKVGAVLSLTGP
                     GAGLGGPERNGILLAEKRINERGGIRGRPLQIIIEDDGSKPDIAKSKAEALIHGEKVV
                     ALLGPSLTASTGAVATVTNSVQMPQLCMTGFGPAIESTYKSLFHMLPPQTLNARAMLE
                     YIAKGLKAKKLGVLHDSGYGQLVMNNIQNFYKDYGIEIVAAEKFEVGATDVSTQAAKV
                     RAAEPDVVGVIATSPVPFRNARQMRVTQPIVSAVGSSSYEYVKGMGEFADDIAFAEFI
                     VSEDPLPHQKEFVDYFHKEKGTLPKTYDAAGWDATHVLAQALEKAGPGAGGEAIAAAI
                     RANRYRGVVAQYNFAAADMTGIELDSYTYSKLVKGQYTRLPFKAGK"
     gene            3567..4454
                     /locus_tag="VE030_03130"
     CDS             3567..4454
                     /locus_tag="VE030_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010808944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZF22441.1"
                     /translation="MSLSLLFQSVLSGLTNGFVYGLVGLGLASIFRGTRIINAMQGDF
                     ALIGGITAVLLLERFGWPMAPAFAAAVAVGGIAGWLMEVLLIRPLRRRGGSEDSYLLL
                     TLGVAFAVSALVLYTVGRDSHSLPGLGGEGIVVIGDAAIRTHALWLIAISLAAMGALR
                     LFFAKTALGLSMMAAALDADGALTTGINVPLMRTLTFVLGGLVGGLAGVLVGPLVTVY
                     FDMGLLLTLKGFAAAILGGLANPMGAIAGGVTLGLLESLAIVTVSSGYKDVIAMAILI
                     VIMIAMPNGLLGRRLRKGG"
     gene            4455..5396
                     /locus_tag="VE030_03135"
     CDS             4455..5396
                     /locus_tag="VE030_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZF22442.1"
                     /translation="MPPLRRALVWGIPLLVWAALPFVATRHIVDLLVFAGLYTIAGLG
                     VAFLLGQCGIVSLAQSVFYGIGAYCTAWFCAHKGLPSPVGAAAGIALSTAIAALVGWP
                     ILRLSGHFLALATLALGIIGQVLFLEWDWLTGGTLGIGGIPRIAPFGFAFDTPLRFYF
                     LVWPIALLLLVLHHNLLHSRNGLALRAMRDSPEAAAVLGLDIHRLKVQMFVLSAALGS
                     AAGSLFAHYVTFVSVDSFAVDRAISFLLLAVLGGVYTVWGPVLGALFITVLPQWLSRL
                     GDIHAFVFAAALIAAVILLPEGFGGAIRNWWRARAPK"
     gene            5414..6169
                     /locus_tag="VE030_03140"
     CDS             5414..6169
                     /locus_tag="VE030_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZF22443.1"
                     /translation="MSPLLQIAQVSHSFGGLHVLRGVDFGVPAGSLTGLIGPNGAGKS
                     TLFNIISGFLAPQRGTVRFDGRDITRDTVQTRSRAGLVRTFQTPKVFGKLSVRENLMI
                     GCYRHLASGVVGNMLRSSRSRGEMELMHARATEAGERFGLSEVMERPAGQVPAGMQRL
                     VELARGYVTRPRLLMLDEPSSGLNTTEIGQLRELLQRLNREGITILLVSHDMQLVDVA
                     SRVHVLCFGSIIASGAMVQIKQDERVRQAYLGV"
     gene            6191..6901
                     /locus_tag="VE030_03145"
     CDS             6191..6901
                     /locus_tag="VE030_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010808940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZF22444.1"
                     /translation="MIEVENLEAGYGETPVLREVSLRLAANETVAVIGANGAGKSTLV
                     AALCGLLPVRAGRIVHLGTDVTRLPAYKRVRRGIGVVLEGRHVFGPMTARQHLALGSL
                     GAREGEKRFGVDDVLALFPFLGERLDDRVELMSGGEQQMVALGRALLLQPQLLILDEP
                     STGLSPLVVKQIMGTIGALRARGMGILLVEQNVALAAEVADRAYVLSVGRVVHEVHKG
                     EWAGFLADDSLVNTYLGG"
     gene            6901..7848
                     /locus_tag="VE030_03150"
     CDS             6901..7848
                     /locus_tag="VE030_03150"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZF22445.1"
                     /translation="MVDIRKIRFFVAVYEAGSITRAAEREHIAQPALTVHMQQIEDEL
                     KVKLFERSAQGVNPTPAGRHFYGICQDLLRRLESVGEEMQAFSGEVAGSIAAGIMPSI
                     CHGPLAPVLARFTATYPNVAVRIVEGLSGTLADWVLSDMVDFAICNRPAATLGLSLRL
                     LVTDRLVLVSARNSGRIPFAPCRLGEIAGLKLVLPSPNHFLRHTLDHHINRGDFRPVV
                     TLEIDGQSATLQFVAHSDWSTILPTIALINEFDSRRFTINPVEEPALSTEIYELRSPR
                     HTPSVASQRFIAMLEAQIQAAPALPAAPGVGGAGHRPAQ"
     gene            7934..9157
                     /locus_tag="VE030_03155"
     CDS             7934..9157
                     /locus_tag="VE030_03155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZF22446.1"
                     /translation="MAPPKVILQLYPMMPAAGEDDRKRRRPLGRDRDAYHRALHDWID
                     IIRAAEDLGVWGASTIEHHLHSEGYEVGPNPGILNAWWASQFKKIHIGALGYVMATQD
                     PIRVAEETAILDHITRGRYFVGFARGYQSRWTNTLGQFTETRAALSDGSSDDERNRQI
                     FEERVNMVLQCWKEDSVRLDGKYYQAPFPPETGIVGYPAHKIARVAGGEGEVDADGAL
                     RRFSVVPAPYQDPHPPIFVAVSRSEPSVRYCARNGFTVVHFSKGDGVAKYARVYQEEA
                     AKHGRSFLFGERQNTVRWPHIAKSTEDFNRKLTQYDLDIYKNFYSPFFPQLPANVDWV
                     QSMKESGLFLGGTLEQARAQFQEEFTKCPSEYLTLIWHYAQQPKDEVIWELEQFMRHI
                     WPTIEAVEPAKAKAA"
     gene            9180..9719
                     /locus_tag="VE030_03160"
     CDS             9180..9719
                     /locus_tag="VE030_03160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HZF22447.1"
                     /translation="MSQAPIDPALLRRALGAFVTGVTVVTTRTSAGDPVGITVNSFNT
                     VSLSPPLVLWSLSLRAASFDAFVQSTHFVVNVLGAHQIPVSERFAMTGGDKFSGVAWR
                     KTLADMPLLDGTAASFTCRNVHRFPGGDHLIFVGEVVAFEQGVCAPLVYANGGYTELR
                     DGSDAARSADKSRNMLASR"
     gene            9757..11559
                     /locus_tag="VE030_03165"
     CDS             9757..11559
                     /locus_tag="VE030_03165"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZF22448.1"
                     /translation="MRHHALALGFLALGCSLNANGAAGTIDDNRLLKAGADLSNWLSH
                     GRDYSNQRFSPLEQINKGNVKRLAPLWTFKSGVSSTFQTTPIVADGVMYLSLPFNHVV
                     ALDAKSGKELWRYQHRRRTEKMCCGPANRGVAVAYGKVFIGTVDARLIALDQNSGKPV
                     WDIALVEDLDGATEKTDQLGADDPLRNSRVSGSTGVGVNMAPLIYRGKVIIGITGVGY
                     GLHLDSERAGAPLGAVIGIAGQSQRAGFYAAFDANTGKKIWQFDSTPTSGWEGKFRET
                     TADGAQLERDIGRERATLAQFPDAAKRGGGSAWTTPAIDPKENLIYVGVGNPSPQMDD
                     LTRPGDNLYTVSLVALDADSGAVKWYYQEVPHDMWGYDVASPPVLFDVTKGYDTIPAV
                     GQAGKTGWFYVMDRRDGRLLFKSDAFVPQQNLFKRPTAEGIEIAPGGAGGSSWSPVSF
                     DTRTGIAFVAGIHWPMRYTVKEIPATVDKAALRYTSLEPGGGPRWGVLSAINTRLSGK
                     IKWQVKTTEPLVGGVLATAGDLLFMGEGNGNFNAFNSETGELLWKINGSAGVNAPPIT
                     YEVDGKQYVAVAAGGNSLFGFKQGDEVMVFALPE"
     gene            11609..12670
                     /locus_tag="VE030_03170"
     CDS             11609..12670
                     /locus_tag="VE030_03170"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HZF22449.1"
                     /translation="MRSKKLEQAVQFAIDHETPWTREITDKWGIHHEDPPPWNKLLGP
                     VHARGPVSGTVVLNGKTLTSWGEPDRADLTFSIAKTYLALLAGVAHDRGLLPDVHEPV
                     RVRVPGIGFEEGRNTQVTWAHLLQQTSEWEGAVFGVPDQADRYRIVQFQGTEAAGKKG
                     DPRSLQTPGTYWEYNDVRINQLSLALLHLFGRPLPEVFRETIMQPIGASDDWRWVGYD
                     NAWINLNGRRVQSVPGGTHWGGGVSIGSHDQARVGQLFLDDGRVNGRQVISAEWIERM
                     RTPCEIAPFYGYLIWLNHDRRVFPSVSASSCFAIGAGGNFTWIEPGQRMVLVVRWIDP
                     DHADAFFGRVLKATDELRS"
     gene            12884..13294
                     /locus_tag="VE030_03175"
     CDS             12884..13294
                     /locus_tag="VE030_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22450.1"
                     /translation="MRPIIFAAILLMLSSATRADENDDIVNGCNFSNAEFGAVAIQIC
                     IKDNQAARAAVLQYAEAVKDIVARCVRGREMGWSIVKLCIDNDIEAEAALAHYGREHD
                     SLMEKCRSEFGRRGAARTKACVDQAIEADRSPRN"
     gene            complement(13355..>14479)
                     /gene="glcF"
                     /locus_tag="VE030_03180"
     CDS             complement(13355..>14479)
                     /gene="glcF"
                     /locus_tag="VE030_03180"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HZF22451.1"
                     /translation="PRGRIYLIKELLEGCTVTAKTQLHLDRCLTCRACETTCPSGVQY
                     GRLVDIGRNIVEKRVGRPFSELLKRAALRAVIPNPALFGLLFRIGQFFRPLLPGALKR
                     KVPKAAARDTVWPEPRHARRMLVLDGCVQPSLAPDINSATARALDRLGISLMKAEAAG
                     CCGAVTFHLNRQENALDYMRANIDAWWPHIEAGAEAVVMTASGCGTMVKEYGHLLTRD
                     PAYAEKAKRISDLTRDISEVIYAERGELEKVLGSPLAPRPSPLAPSAAKPRVAFHSPC
                     SLQHGLQIRGKVEDLLTAAGFELTSVPDSHLCCGSAGTYSILQRELSQKLLKNKVNAL
                     ESGTPALLATANIGCLTHIQSGTALPVRHWIELLDERLKN"
BASE COUNT         2540 a         4488 c         4779 g         2672 t
ORIGIN      
        1 cggcacctgc cggaaatcta cgccgtggaa acggcgctat tggcgtaggc gggccctgcc
       61 ccgatgaggg tgctcatacc cagcgcgttg cgctcttata ccgagcgcag cgaggccgag
      121 gcgggcggcg cgacgctcgc cgccgtcctt gcggacctgg accgtcaata ccccggaatt
      181 cgtttccgca tgatcgacga gcaagacagg attcggcctc acatccgcat cttcgtcaac
      241 ggcgagcagg tgcgcgaccc gtcgcagctg ttgcgcgcga ccgacgaggt cgtcatcgtt
      301 caggcgctca gcggtggttg atattgcgca cgcgatgtct gcccgcgggc catgcgataa
      361 actcgcgtcg atgccgcccg tcgtttccat ttcgaacctg gccaagacct acgcctccgg
      421 cctccaggcg ctgaagaagg tcgacctgga agtccgcaag ggcgagatct tcgccctgct
      481 tggcccgaac ggtgccggca agaccacgct gatcagcatc atttgcggga tcgtcacgcc
      541 gacctcgggc accgtgctgg tggacggccg cgacatcttg cgcgactacc gcgagtgccg
      601 ctcgaagatc gggctcgtgc cccaggaatt gcaaaccgac atgttcgaga cggtgtggaa
      661 caccgtgaac ttcagccgcg ggctgttcgg tcgcgcgccc gacccggcgc atctcgcgaa
      721 agtactgcgc gacctgtcgc tgtgggacaa gcgcaacgac aggatcatga ccctgtccgg
      781 cggcatgaaa cggcgggtac tgatcgccaa ggcgctggct cacgagccgg agatcctgtt
      841 cctggatgag ccgacggcgg gcgtggacgt ggagttgcgg cgcgacatgt ggacgctggt
      901 gcagcggctg cgcgccggcg gcgtgaccat catcctgacc acgcactaca tcgacgaagc
      961 ggaggaaatg gccgaccgca tcggggtcat cagcaagggc gagctgatcg tggtcgagga
     1021 gaaaacccgg ctcatgaaga agctcggcaa gaagcagctc accttgcatc tgctggagcc
     1081 gatgacggcg attcccgcgg agctcggcac ctggagcctg gtgctgaaga cggaaggcac
     1141 cgtgctggaa tacaacttcg atgcggccga ggaaggtacc gggattcccg cgttgctgcg
     1201 ccgcctgagc gagctcggca ttggcttcaa ggatcttcag accgaacaaa gctcgctgga
     1261 ggacattttc gtcagtctgg tggccaagaa ttctgagggc gggcgcgcat gagcacggca
     1321 gcgaaaatcc cgtcgttcaa ccgccacggc gtgctggcca tctaccgctt cgagatggcg
     1381 cgcttcaaac gcaccgtatg gcagagcctg gtgacgccgg tgatcaccac atcgctctat
     1441 ttcgtggtgt tcggtgccgc gatcggctcg cgcatcagcg aagtgggcgg cgtgccctac
     1501 ggctccttca tcgtgccggg gctgatcatg ctgtcgcttt ttaccgagag cctcaacaat
     1561 gcctcgttcg gcatttatct gccgcggttc accggcacta tttacgaagt cctgtcggcg
     1621 ccggtgtcgg cgctcgagat cgtgctcgcc tatgtaggcg cggcggcgac caagtcggtc
     1681 atgctcggcc tggtgatcct ggcgacggcg atgcttttcg tgccaatgcg gatcgagcac
     1741 ccgctatgga tgatcgcctt cctggtgctt accgcggcga cgttcagcct gttcggcttc
     1801 atcatcggta tctgggcgaa tggcttcgag cagttgcagt tcatcccgat gctaatcatc
     1861 acgccgctga cctttctggg cggggccttc tattcgatcg atatactgcc gcccgcgtgg
     1921 cgcgcggtga gcctgttcaa tccgatcgtt tacctcgtca gcggcttccg ctggagcttc
     1981 tacagcacct ctgacgtcgg cgtgggagtc agtctcgcag caacgctcgc cttcttcgta
     2041 ctgtgtttgg ccgtggtggt gtgggttttc aggacggggt atcgtctgaa aagctgaagc
     2101 ggcaggcatt ccgcagcaaa tcgggccgcc aggcccttcg tcatcgtgag ctacaaattc
     2161 gccagggaca tggttttgcg tatttcgggg caactcctca cgccggcgca aaatgttccg
     2221 ggcttgcggc atactactgc ccggggtgtg gaggggcgcg acgtccgcgg agcgggcgcg
     2281 caaagcaaaa ttccaacaat tcaaggaggc ttcacccatg atgcggattt tggtgcaggt
     2341 attctgccgg ccgtcgctgc tgctggcgct gttgtcgggc atcgcggcgg cgcaacaacc
     2401 ggccggcgag ccgataaagg tcggtgcggt gctgtcattg accggccccg gcgcggggct
     2461 cggcggtccg gagcgcaacg gtatcctgct tgccgagaaa cgcatcaacg agcgcggcgg
     2521 catccgcggc cgcccgctgc agatcatcat cgaggatgac gggtcgaagc ccgacatcgc
     2581 caagagcaag gccgaagccc tgatccacgg cgagaaggtg gtcgcgctgc tcggcccttc
     2641 gctgaccgcg agcaccggcg cggtggccac cgtgaccaac agcgtgcaga tgccgcagtt
     2701 gtgcatgacc ggattcggcc cggcgatcga atcgacctac aagtcgctgt tccacatgct
     2761 gccgccacag acgctgaacg cgcgcgccat gctggaatac atcgccaagg gcctgaaggc
     2821 gaagaagctc ggcgtgctgc atgactccgg ttacggtcag ctggtgatga acaacatcca
     2881 gaacttctac aaggactatg ggatcgagat cgtcgccgcg gagaaattcg aagtcggcgc
     2941 gaccgacgtg agcacacagg cggccaaggt ccgcgccgcc gagccggatg tggtgggcgt
     3001 gatcgccacc tcgccggtgc cgttccgcaa cgcccgtcag atgcgcgtca cccagccgat
     3061 cgtttccgcg gtcggctcct cctcgtacga atacgtgaag ggcatgggcg aattcgccga
     3121 cgacatcgcg ttcgcggagt tcatcgtctc cgaagacccg ctgccgcacc agaaggaatt
     3181 cgtcgactac tttcacaagg agaagggcac gctgcccaag acctacgacg cggcgggatg
     3241 ggacgcgacg catgtcctgg cgcaggcgct cgagaaggcc ggtccgggtg cgggaggcga
     3301 ggccatcgcc gccgcgatcc gcgccaaccg ctaccgcggc gtggtcgccc aatacaactt
     3361 cgccgccgcc gacatgaccg gcatcgaact ggacagctac acctattcga agctggtgaa
     3421 ggggcaatac acgcgccttc ccttcaaggc cggcaagtag ggggtgcagt gggtggccgg
     3481 gtccggcgcg ggagcggtct gcttcaacgc ggctggcgag gacctgtgcg catgcagacc
     3541 ggaagcccga tgaccgcacc atcgcgatga gcctgagcct tctcttccag tcggtccttt
     3601 ccggcctgac caacggtttc gtctacggac tggtggggct ggggctggca tcaattttcc
     3661 gcggcacgcg cattatcaac gccatgcagg gcgacttcgc gctgatcggc ggcatcaccg
     3721 ccgtgctgct gctcgagcgc ttcggctggc ccatggcgcc ggcattcgcc gccgcggtag
     3781 cggtcggcgg aatcgcgggc tggctgatgg aagtgctgct gattcgcccg ttgcgccggc
     3841 gcggcggctc ggaggacagc tacctgctgc tcacgctggg tgttgccttc gcggtttccg
     3901 cactggtcct gtacaccgtc ggccgggatt cgcattcgct gcccggcctc ggtggcgaag
     3961 gcatcgtcgt gatcggcgat gccgcgatcc gtacgcatgc gctgtggctg atcgcgatca
     4021 gcctcgcggc gatgggcgcg ctgcggctgt tcttcgcgaa gactgcgctc ggcctgtcga
     4081 tgatggccgc cgccctggac gccgatggcg cactcaccac tggcatcaac gtaccgctga
     4141 tgcgcacgct tactttcgtg ctcggtggac tggtcggcgg gctcgccggc gttctggttg
     4201 gcccgctcgt gacggtatat ttcgacatgg gcctgttgct cacattgaag gggttcgccg
     4261 ctgcgatcct cggtgggctg gccaatccga tgggcgcgat cgccggcggc gtcacgctcg
     4321 ggcttctcga atcgcttgcg atcgtcacgg tttcctccgg ttacaaggac gtgatcgcga
     4381 tggctatcct gatcgtcatc atgatcgcca tgcccaatgg cttgctgggc cggcggctgc
     4441 gcaagggggg ctgaatgccg ccactgagac gcgcgctggt ctggggcatc ccgttgctgg
     4501 tatgggcggc cctgccgttc gtcgccacgc gccacatcgt cgatttgctg gtattcgcgg
     4561 gcctctacac catcgccggt ctcggggtcg cgttcctgct cggccagtgc ggcatcgtca
     4621 gccttgcgca aagcgtcttc tatggcatcg gtgcctactg caccgcctgg ttctgcgcgc
     4681 acaaggggct gcccagcccg gtgggcgcgg cagcgggcat cgcattgagt accgccatcg
     4741 ccgctctggt cggctggccg atcctgcggc tttccgggca cttcctcgcg ctcgccacgc
     4801 tcgcgctggg catcatcggg caggtgttgt tcctggagtg ggactggctc accggcggca
     4861 cactgggcat cggcggcatc ccgcggatcg cgcccttcgg gttcgcgttc gacacaccgc
     4921 ttcgtttcta tttcctggtc tggccgatag ccttgttgct gctggtgctg caccacaacc
     4981 tgctgcattc gcgcaacggg ctcgcgctcc gtgcgatgcg cgattcaccc gaggccgcgg
     5041 ccgtgctcgg cctggatatc cacaggctga aggtgcagat gttcgtgctg agcgccgcgc
     5101 tcggctcggc tgcgggcagc ctgttcgccc attacgtgac cttcgtcagc gtggacagtt
     5161 tcgcagtcga ccgtgcgatc agctttctgc tgctggcagt gctgggcggg gtgtacaccg
     5221 tatggggacc ggtactcggt gcgctgttca tcaccgttct cccgcagtgg ctgagccggc
     5281 tgggggacat tcatgccttc gtgttcgccg cggcgctgat cgccgccgtg atcctgctgc
     5341 ccgagggttt cggcggcgcg atccgcaact ggtggcgcgc gcgtgcgccg aaatgaccat
     5401 ggggacggaa acggtgagcc cgctcctgca aatcgcacag gtctcgcaca gtttcggcgg
     5461 cctgcacgtg ttgcgcggcg tggacttcgg cgtgccggcc ggatcgctca ccggccttat
     5521 cggaccaaat ggcgcgggga aaagcacgct gttcaacatc atttccggtt tcctggcacc
     5581 gcagcgcggc acggtgcggt tcgacgggcg cgacatcaca cgcgacacgg tgcagacccg
     5641 cagcagggcc gggctggtgc gcaccttcca gaccccgaag gtgttcggga aattgagcgt
     5701 gcgggagaac ctgatgatcg gctgctatcg ccacctcgca tccggcgtgg tgggcaacat
     5761 gctgcgctca agccgttcgc gcggggagat ggagctgatg catgcgcggg ccacggaggc
     5821 cggcgagcgc tttggcctga gcgaggtgat ggagcggccg gccggccaag tgccggcggg
     5881 catgcagcgg ctggtcgagc tggcccgcgg ctacgtcacc cggccgcggc tgctgatgct
     5941 cgacgaaccg tcctcgggac tgaacaccac ggagatcggc cagttgcgcg aactgctgca
     6001 gcggctgaac cgcgaaggca tcaccatcct gctggtgtcg cacgacatgc agctggtgga
     6061 cgtggcgagc cgcgtgcatg tgctgtgctt cggctcgatc atcgcctcgg gcgccatggt
     6121 gcagatcaaa caggacgaac gcgtgcgcca ggcctatctc ggggtctaga gcctatggaa
     6181 ccgtgcggcg atgattgaag tcgagaacct ggaagccggc tacggtgaga cccctgtgct
     6241 gcgggaggtg tcgctgcgcc tggcggccaa cgaaaccgtt gcggtgatcg gggccaatgg
     6301 cgcgggcaag agcacgctgg tggcggcgct gtgcggcctg ctgccggtgc gcgccgggcg
     6361 tatcgtgcat ctgggaacgg atgttacccg gcttcccgcg tacaagcggg tgcggcgcgg
     6421 catcggcgtg gtgctcgaag gccgtcacgt gttcggcccg atgaccgcgc gccagcatct
     6481 cgctctgggc agcctgggcg cgcgggaagg cgagaaacgc tttggcgtcg atgacgtgct
     6541 ggccctgttc ccgttcctgg gtgagcgtct cgacgatcgc gtcgagctga tgagcggcgg
     6601 ggagcagcag atggttgcgc tcgggcgcgc gctgctgttg cagccgcagc tgctgatcct
     6661 ggacgagccc tccaccgggc tctcgccgct ggtggtaaag cagatcatgg ggaccatcgg
     6721 cgcgctgcgc gcgcgcggca tgggcatttt gctggtggag cagaatgtgg cgcttgccgc
     6781 cgaagtggcc gatcgcgcct atgtgctttc agtcgggcgc gtggtgcacg aagtgcacaa
     6841 gggcgagtgg gcgggcttcc tcgccgacga cagcctggtc aatacctatc tgggcggctg
     6901 atggtcgata tccggaaaat ccgcttcttc gtcgccgtct acgaggccgg gagcatcacc
     6961 cgcgccgcgg agcgcgagca catcgcccag cccgcgctca cggtccacat gcagcagatc
     7021 gaggacgagc tgaaagtgaa attgttcgag cgctccgcgc agggcgtgaa cccgacgccg
     7081 gcggggcggc atttctacgg catctgccag gacctgctgc gccggctgga gtcggtcggc
     7141 gaggaaatgc aggccttcag cggcgaagtg gcggggtcga tcgcagccgg catcatgccc
     7201 tcgatctgcc acgggccact ggcgccggtg ctggcacgct ttaccgcaac ctatccgaat
     7261 gtcgcggtgc gcatcgtgga gggcttgagc ggcacgctgg ccgactgggt gttgtccgac
     7321 atggtcgatt tcgcgatctg caaccgtccg gccgcgaccc tcggattgtc gctgcgcctg
     7381 ctggtgaccg accggctcgt tctggtctcg gctcgcaaca gcgggcgcat accgttcgcc
     7441 ccgtgccgcc tgggcgagat tgccggcctg aaactggtgc tgccttcgcc caaccacttc
     7501 ttgcggcaca cgctggacca tcacatcaac cgcggcgact tccggccggt ggtcacactg
     7561 gagatcgacg ggcagagcgc cacgctgcag ttcgtggcgc attcggactg gtcgacgatc
     7621 ctgccgacca ttgcgctgat caacgagttc gactctcggc gtttcaccat caatccggtc
     7681 gaggagccgg cgctgtccac cgagatctac gaattgcgct ccccgcgaca cacgccgagc
     7741 gtcgcctcgc aacgattcat cgccatgctc gaagcacaga ttcaggcggc cccggccctg
     7801 cccgccgcgc cgggcgtcgg cggggcgggg catcgtccgg cgcaatgacc gtcatagcca
     7861 accactattg gagggcgacg atcacgccgg cctaaactgc ggtgagcttc caatgaacac
     7921 ccgaggaaaa aacatggctc cgcccaaagt gatactccag ctctacccca tgatgcccgc
     7981 cgctggcgag gacgaccgca agcgccgccg tccgctgggg cgcgaccgcg acgcgtacca
     8041 ccgcgcactg cacgactgga tcgacatcat ccgcgcggcg gaagatctcg gggtctgggg
     8101 cgccagcacc atcgagcacc atctgcattc tgaaggctac gaagtcgggc ccaacccggg
     8161 tatcctcaat gcgtggtggg cctcgcagtt caagaaaatc catatcggtg cgctcggcta
     8221 cgtgatggcc acgcaggacc cgatccgggt ggcggaggaa acggcgatcc tggaccacat
     8281 cacccgcggc cgctatttcg tcggcttcgc gcgcggctac cagtcgcgct ggaccaacac
     8341 gctcgggcag ttcaccgaaa cccgggcggc gctttcggac ggcagcagcg acgacgagcg
     8401 caaccgccag atcttcgagg aacgcgtgaa catggtgctg cagtgctgga aggaggattc
     8461 ggtgcgcctc gacggcaagt actaccaggc gccgttcccg ccggaaacgg gcattgtggg
     8521 ttaccccgcg cacaagatcg cgcgcgtggc cggcggagaa ggggaggtcg atgctgacgg
     8581 cgcgctgcgc cgcttcagcg ttgtccctgc gccgtatcag gacccgcatc cgccgatctt
     8641 cgtggcggtg agccgcagcg agccttcggt gcgctactgc gcgcgcaatg gtttcacggt
     8701 ggtgcacttt tccaagggcg acggcgtggc gaagtacgcg cgcgtctacc aggaggaagc
     8761 ggcgaaacac gggcgcagct tcctgttcgg cgagcgccag aacacggtgc gctggccgca
     8821 catcgcgaag agcaccgaag acttcaaccg gaaactcacg cagtacgacc tcgatatcta
     8881 caagaacttc tattcgccgt tctttccgca actgcccgcc aacgtcgatt gggtgcagag
     8941 catgaaggaa tccggtctgt tcctcggcgg cacgctggag caggcgcggg cgcagttcca
     9001 ggaggaattc accaagtgcc cgagcgaata cctcacgctc atctggcact acgcccagca
     9061 acccaaggac gaagtaattt gggagctgga gcagttcatg cgccatatct ggccgacgat
     9121 cgaagcggtc gaaccggcca aggccaaggc ggcgtgaagt gaatccggcc ggttcgccca
     9181 tgagccaggc gccgatcgat ccggcgctgc tgcgccgcgc gctcggtgca ttcgtcaccg
     9241 gcgtcaccgt ggtgaccacg cgcacgtccg ctggcgatcc ggttggcatc accgtgaatt
     9301 ctttcaatac cgtgtcgctt tcgccgccgc tggtgctctg gagcctgtcc ctgcgcgctg
     9361 caagtttcga tgcgttcgtg cagtcgaccc atttcgtcgt gaacgtgctg ggcgcgcacc
     9421 agattccagt ctccgagcgc tttgcgatga ccggcggcga caaattctcc ggcgtggcct
     9481 ggcgcaagac gctcgcggac atgcccttgc tggacggcac ggcggcgagc ttcacttgcc
     9541 gcaatgttca ccgctttcct ggcggggacc acttgatctt cgtcggcgaa gtcgtcgcct
     9601 tcgagcaggg tgtgtgcgcg ccgctggtgt acgccaacgg cggctacacc gagctgcgcg
     9661 acggttccga tgcggcccgc tccgccgata aatccaggaa catgctcgcc tcgcgctagc
     9721 gctactccga agctttcagg aaataggaga tcgatcatgc gtcatcacgc attagcgctt
     9781 ggtttcctgg ctctgggttg ttcgctcaac gccaatggcg cggcggggac catcgacgac
     9841 aaccggctgc tcaaagctgg ggcggacctg agcaactggc tgagtcacgg ccgggattac
     9901 agcaatcagc gcttctcccc actcgagcaa atcaacaagg gcaacgtcaa gcgcctggcc
     9961 ccgctctgga ccttcaagag cggcgtgtca tccaccttcc aaaccacgcc tatcgtggcg
    10021 gacggtgtga tgtacctgtc cctgccgttc aaccacgttg ttgcactgga cgcgaagtcc
    10081 ggcaaggaac tgtggcgtta tcagcacagg cggcgcacgg agaaaatgtg ttgcggtccg
    10141 gccaatcgtg gcgtagcggt ggcctatggc aaggtgttca tcggcacagt ggatgcgcgt
    10201 ttgatcgcgc tggatcagaa cagcggcaag ccggtctggg atattgcgct ggtggaggac
    10261 ctcgacggcg cgacggagaa gaccgatcag cttggtgccg atgatccgct gcgcaacagt
    10321 cgcgtaagtg ggtccaccgg cgtgggcgtg aatatggccc ccctgatcta caggggcaag
    10381 gtcatcatcg gcatcaccgg cgtgggctac ggactgcacc tggattccga acgtgcgggt
    10441 gcgccgctcg gggcagtgat cggtatcgcg ggacagagtc agcgagcggg tttctacgcg
    10501 gcattcgacg ccaataccgg aaagaagatc tggcaattcg actccacgcc gacttccggg
    10561 tgggaaggta agtttcgtga gactactgcg gacggtgccc aactggaacg agacatcgga
    10621 agagaacgcg ccacgcttgc gcagtttccg gacgcggcaa aacgcggtgg cgggtcggcc
    10681 tggaccactc ctgccatcga cccgaaggaa aatctgatct atgtcggtgt cggcaacccc
    10741 tcgccgcaga tggatgattt gacgcgaccg ggtgacaacc tctacacggt gtctctcgtg
    10801 gcactggacg ccgactcggg cgccgtcaag tggtactacc aggaggtccc gcacgacatg
    10861 tggggctacg acgtggcaag tccccccgtg ctgtttgatg tgaccaaggg ctacgacacg
    10921 attccggccg taggccaggc gggcaaaacg ggatggttct acgtgatgga tcgccgcgac
    10981 ggccggttgc tattcaaatc ggacgctttc gtgccgcaac agaatttgtt caagcgaccg
    11041 accgcggaag gcatcgagat cgcgccaggg ggtgcgggag gctcaagttg gtcgccggtt
    11101 tcgttcgaca cgcgcacggg gatcgccttt gtcgccggga ttcactggcc catgcgttac
    11161 acggtgaagg aaattccagc gaccgtggat aaggctgctc tgcgatacac gtcgctggag
    11221 ccgggcggtg ggccgcgctg gggcgtgctg agcgccatca acacccggct ctccggcaag
    11281 atcaagtggc aagtgaaaac aactgagccg ctggtcggcg gcgtgcttgc caccgcgggt
    11341 gacctgcttt tcatgggtga gggcaatggc aatttcaacg ccttcaactc cgaaaccggc
    11401 gagttgctct ggaagatcaa cggtagtgcg ggtgtgaacg caccgcctat tacttacgag
    11461 gtcgacggaa agcagtacgt cgcggtcgcc gctggaggca actcgctgtt cggtttcaag
    11521 cagggagacg aagtgatggt attcgccttg ccagagtagg agttcctgaa gcgcgccgaa
    11581 aggaattcac gcgccaggtg catcttccat gagaagcaag aaactcgagc aggccgtgca
    11641 atttgcgatc gaccacgaaa ccccgtggac gcgcgagatc accgacaaat ggggtattca
    11701 tcatgaggac ccgccaccct ggaacaaact gctcggcccg gtgcatgcgc gcggcccggt
    11761 gtccggaacc gtcgtactga acggcaaaac cctgacctcc tggggcgagc cggatcgcgc
    11821 cgacctcacc ttcagcattg cgaaaaccta ccttgcactt ctcgccgggg ttgcccacga
    11881 ccgcgggttg ttgccggatg tgcacgaacc ggttcgagtg cgtgtacctg gcatcggctt
    11941 cgaagaaggc cgcaacacgc aagtgacctg ggcgcatctg ctgcaacaga ccagcgagtg
    12001 ggaaggcgcg gtcttcggcg ttccggatca ggcggaccgc tatcgcatcg tgcagtttca
    12061 gggaaccgag gcggcgggca agaaaggcga cccgcgatca ctgcagactc cgggcaccta
    12121 ttgggaatac aacgacgtgc gcatcaacca actttcgctg gccttgttgc atttgtttgg
    12181 tcgtccgttg ccggaggtgt ttcgcgaaac catcatgcaa cccatcggcg ccagcgatga
    12241 ctggcgctgg gtcgggtacg acaacgcatg gatcaatctc aacgggcgca gggtccagtc
    12301 ggtccccggc ggcacgcatt ggggtggcgg cgtatcgata ggcagccacg accaggcgcg
    12361 cgtcggccag ttgttcctgg acgacggcag ggtgaacggc cggcaggtaa tatcagcaga
    12421 atggatcgag cgcatgcgca caccgtgcga gatcgcgccg ttctacggct atctgatctg
    12481 gctcaaccac gaccgccgcg tgtttccgag cgtgtctgca tcgagctgct tcgccatcgg
    12541 cgcaggcgga aacttcacgt ggatcgagcc cgggcagcgc atggtactgg tggtgcgctg
    12601 gatcgatccg gaccacgccg acgcgttctt cggacgagta ttaaaggcga ccgacgaact
    12661 gcgatcgtag caataggata actgaatcgg ccgccggcag aaggttcctt caggcctcag
    12721 gatcaccctg cgccactttg ccgaaccaat cctcgatggt ggccaggccg cctgcctgtt
    12781 gcgcgcgccc ctggtccttg cctggcaaat cttgctgaag catgtcgatt ttgttggagc
    12841 aaaatagaat cttctggtcc aacctgtcgg agagtccttg cccatgagac caattatttt
    12901 cgctgccatc ttgctgatgc tttcctctgc tacccgggca gatgagaacg acgacatcgt
    12961 caacggctgc aacttctcaa acgccgagtt tggcgctgtg gcaattcaga tttgcataaa
    13021 agacaaccag gcggcccgcg cagcggtgtt gcagtacgcg gaagcggtca aggacatcgt
    13081 cgcgcgctgc gtgcgcggga gggaaatggg ctggtcgata gtgaagctgt gcatcgacaa
    13141 cgacattgaa gcagaggctg cgctcgccca ttacgggaga gaacacgact cgctgatgga
    13201 gaaatgcagg tccgagttcg ggcgccgggg cgcggcgaga accaaagcgt gcgtggatca
    13261 ggcaatcgag gctgacaggt cgcccaggaa ctgagaagcg ggcgtgaacc aatatggcgg
    13321 cggtaagact tcgcgattgc cggttcggcc gcgctcagtt tttcagtctc tcgtccagca
    13381 gttcgatcca atgcctgacg ggcagggcgg tgccgctctg aatgtgcgtc aggcatccga
    13441 tattcgccgt cgcaagtagt gccggcgttc ccgactcaag cgcgttgacc ttgttcttga
    13501 gcagtttctg cgacagctcc cgctggagaa tcgaataggt ccccgccgag ccgcagcaca
    13561 ggtgtgaatc cggcaccgaa gtcagttcga agcccgcagc agtgagcagg tcttccacct
    13621 tgccgcggat ctggaggcca tgctgcaacg aacacggcga gtggaaggcg acacggggct
    13681 tcgccgcgct aggggcgagg ggcgagggtc gaggggcgag gggagaacct aaaacttttt
    13741 cgagttctcc acgctctgcg tagatgacct cgcttatgtc tctggtcagg tccgatatcc
    13801 gcttcgcttt ttcggcgtag gccgggtcgc gggtgaggag gtggccgtac tccttgacca
    13861 tcgtgccgca accgctcgcg gtcatgacca ccgcttccgc gccggcctcg atgtgcggcc
    13921 accaggcgtc gatgtttgca cgcatgtagt cgagcgcgtt ttcctggcgg ttcaaatgga
    13981 aggtcacggc gccgcagcag cccgccgctt cggctttcat cagcgaaatt cccaaccgat
    14041 ccagcgcacg tgccgttgcc gaattgatgt cgggcgcaag cgagggttgc acgcatccgt
    14101 ccaggaccag catcctgcgc gcgtggcgcg gctcaggcca gacggtgtcg cgtgcggcgg
    14161 ccttcggcac tttgcgtttg agcgcgccgg gcaacagcgg ccgaaaaaac tggccgatcc
    14221 tgaacaggag gccgaacagc gccgggttcg ggatcacggc acgcagcgcg gcccgcttga
    14281 gcaattcgct gaaaggccgg cctacgcgtt tttcgacgat gttgcggccg atgtccacca
    14341 ggcgcccgta ctgcacgccg gacgggcagg tggtctcgca ggcgcgacag gtcaggcagc
    14401 gatccaggtg caactgcgtt ttggcggtga cggtacaacc ctcaagcaat tccttgatca
    14461 ggtagatgcg tccgcgcgg
//
LOCUS       DASQOP010000081         6342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2042567, whole genome shotgun sequence.
ACCESSION   DASQOP010000081 DASQOP010000000
VERSION     DASQOP010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6342
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2042567"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..1019)
                     /locus_tag="VE030_03185"
     CDS             complement(66..1019)
                     /locus_tag="VE030_03185"
                     /inference="COORDINATES: protein motif:HMM:NF022834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HZF22452.1"
                     /translation="MFADSIGARVPSRARAQVHSVFLRACNIETESGELVTLLAAGSG
                     NLPHGIRCASPIAPLCARLRQGQTAILEGATLRVPTADFVVDFSRAAVWSGTVAAALP
                     GVRGAEPQGALRELWETLREHAPDQGVAPALFSSGSRRSMLGRALVARLAQTLPILAR
                     ATEAGDAGAVVSTLGALVGLGAGLTPAGDDLIVGYLAALWSRSHREPGIAALLRALVV
                     PVGQLSLHTNAISRQMLLDALRGHFAERLTEVVRCVCGGGNVAGASMRALEIGHSSGA
                     DVLCGLLFGYSPTLTAGSVLACATENYSGDCHAARVAAIAC"
     gene            complement(1077..1748)
                     /locus_tag="VE030_03190"
     CDS             complement(1077..1748)
                     /locus_tag="VE030_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HZF22453.1"
                     /translation="MANKAIDAEPYELEFDPKTTALLIIDMQRDFVMPGGFGEALGND
                     VTPLQATIAPTKRVLESARKRGMLVIHTREGHRPDLTDCPPTKLVRGRGKTRIGDPGP
                     MGRILVRGEIGHDIVPELYPAPGEPVIDKPGKGAFYATDLELILRDRGIKTLIVCGVT
                     TEVCVNTSVREANDRGYECVVLSDCVGSYFPEFQKAALAMIKAQGGIFGWVSDSRRAL
                     AALGA"
     gene            1887..3677
                     /gene="atzF"
                     /locus_tag="VE030_03195"
     CDS             1887..3677
                     /gene="atzF"
                     /locus_tag="VE030_03195"
                     /EC_number="3.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004039 - allophanate hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase"
                     /protein_id="HZF22454.1"
                     /translation="MTISSLLADYRSGKRTPGAVVESLVSGDDETYACAWICKVGPDV
                     LARRARQLEQALREDPRAIDRLPLFGVPFAVKDNIDVAGLPTTAACPAFSYVPERSAT
                     AVEKLEAAGAILVGKTNLDQFATGLVGTRSPYGAVPNAFRPEYISGGSSSGSAVTVAR
                     GQVSFTLGTDTAGSGRVPAGFNNIVGLKPSRGLISARGVVPACQSLDCVSVFALTVPD
                     AVAVLGVARGPDPEDPWSRTLNLSVPPLAERFTFALPGPLEFYGDDHARHAFHDAVDK
                     LEGLGGKPIGVDFSVFLETAALLYEGPWVAERLAAIRPFFESHPDQVHPVVRKIIEGA
                     SGFSAADLFSGLTKLEALRKRAAGLWDRADVLVVPTSPTIYTIKEVLAEPFLNNRRLG
                     HYTNFVNLMDLAACAVPASMRPDGLPCGITLIAPAGTDLMLANLAQRFHHLTGLKLGA
                     LDEVLPPPAAIATGDDSVRVAVVGAHLSGLPLNHQLTGRGARLEKPARTTARYRLFAL
                     PGTTPPKPGMVRDADKGSVIDVEVWRLPVAEYGSFVAGIPSPLGIGQVELDDGEWVQG
                     FLCESWAVNGAEEISHLGGWRAYLASKAAR"
     gene            3674..4417
                     /locus_tag="VE030_03200"
     CDS             3674..4417
                     /locus_tag="VE030_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZF22455.1"
                     /translation="MKRTGNRGRSATVRSTAGKRRKVTTDEERKPSLAESVYLGIKQD
                     IFDFRLLPGDRFTENEVAGRLNVSRTPAREALYRLAREGYLKVHYRSGWSVRELDFKQ
                     FDDLYDLRIALETAAVKQLCDEAGRPNLEDLKSIWLVQPETRLDDGERVCALDEAFHC
                     ALVAATGNREMARCHQDVTERIHLLRRLDFTEAGRIAATYQEHAQILRAVLRQDADRA
                     IGLLQAHIGTSKIEVRKISLHKLLSARRA"
     gene            complement(4467..4799)
                     /locus_tag="VE030_03205"
     CDS             complement(4467..4799)
                     /locus_tag="VE030_03205"
                     /inference="COORDINATES: protein motif:HMM:NF023171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3301 domain-containing protein"
                     /protein_id="HZF22456.1"
                     /translation="MIELATLIIFVAGAWFWTDSMRAREAALDAGRRACAAEGVQLLD
                     WTVMLKQTRLGRDDEGRVRVKRVYEFEFSDTGDNRIKGSITLLGRELLALNLPIAPLI
                     PSNVVQLH"
     gene            complement(4933..5541)
                     /locus_tag="VE030_03210"
     CDS             complement(4933..5541)
                     /locus_tag="VE030_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HZF22457.1"
                     /translation="MKPPPFTREWLAERLSRQGILPEDANFPRYAHPPEREPVPAAVL
                     VPVVNQSAGPTLLFTQRTAHLHDHAGQISFPGGRVDEGDPDRIATALREAEEETGLSR
                     QHVEIIGRLPDYDILTGFRVTPIVGWVEPPFDLSPDPFEVADVFEVPLEFFLDPVNHQ
                     RHSDVFNGRTRHFYSMAYGDRYIWGATAGILYTLYQILTGRS"
     gene            5706..5951
                     /locus_tag="VE030_03215"
     CDS             5706..5951
                     /locus_tag="VE030_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22458.1"
                     /translation="MKLRSLIAAVMFAMAGSAFAMHCPMDMKKIDEALAKKPEISASK
                     LAEVKKLRAEGEKLHNAGKHQESVDTLAKAMAILGIK"
     gene            complement(6046..>6342)
                     /locus_tag="VE030_03220"
     CDS             complement(6046..>6342)
                     /locus_tag="VE030_03220"
                     /inference="COORDINATES: protein motif:HMM:NF023763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3641 domain-containing protein"
                     /protein_id="HZF22459.1"
                     /translation="EFLIESGNYEGYMERLANAFNPAAAAGVMCRYTLSVGWEGTLYD
                     CDFNQMLDLPLANGTPRHIRDFDLAKLNGRRIVTANHCYGCTAGSGSSCGGAVT"
BASE COUNT         1167 a         2052 c         2022 g         1101 t
ORIGIN      
        1 ccgagtcccg gtactccctg ggcacgattt tcaccagcgt ggccgccaag tcccgttctc
       61 ccgcatcaac aggcgatcgc tgccacgcgc gcggcatggc aatcgcccga ataattttcc
      121 gtggcgcagg ccagcaccga ccccgcagtc agcgtgggtg aatatccgaa cagcagcccg
      181 cataaaacgt ccgcacccga actgtgtccg atttccagcg cccgcatgct ggcgccagcg
      241 acgttgccgc caccgcaaac gcagcgcacg acttcggtca ggcgttctgc gaaatgaccg
      301 cgaagcgcgt cgagcaacat ctggcggctg atcgcgttgg tatgcagcga caactgcccg
      361 acaggcacta cgagcgcgcg cagcagcgcg gcgatgccag gttcgcggtg cgagcggctc
      421 cagagtgccg cgagatagcc gacgatcaag tcgtctcccg ccggagtgag cccggcgccg
      481 agacccacca gcgcgcccaa ggtcgaaacg actgcgccgg catcgcccgc ttcggtcgcg
      541 cgcgccagaa tcggcagcgt ttgcgccagg cgcgcaacca gcgcgcgccc gagcatggag
      601 cgacggctgc cggacgagaa tagcgcgggc gcgacgccct gatccggtgc gtgctcgcgc
      661 agcgtctccc acaattcacg gagcgcccct tgcggctccg cgccacgcac gccgggcaac
      721 gcggcagcga ccgttccgct ccagactgcg gcgcgggaga agtccaccac gaaatccgcg
      781 gtgggaacac gcaacgtcgc gccctcgagt atcgccgtct gcccctggcg cagacgcgcg
      841 caaagcggcg cgatcggcga ggcgcagcgg attccgtgcg ggagattgcc ggagccggct
      901 gcgaggagcg ttaccagttc gccggattcc gtttcgatgt tgcaggcgcg caggaacacg
      961 ctgtgtacct gcgcgcgggc gcgcgacggc acgcgcgcgc cgatgctgtc tgcaaacaag
     1021 actttccctt cgtccgtcac ggagccgccc gaatgcgccc taccgcgtag ctgttctcac
     1081 gcaccgaggg cggccagcgc tcggcgggag tccgacaccc agccgaagat accgccctgg
     1141 gccttgatca tggcgagcgc agccttctgg aactccggga aatacgaccc gacgcaatcg
     1201 gagagcacca cgcattcgta gccgcggtcg ttggcttcgc gcacgctggt gttgacgcag
     1261 acttcggtcg tgacgccgca tacgatcagc gtcttgatgc cgcggtcgcg caggatgagc
     1321 tcgaggtcgg tggcgtagaa cgcgcctttg ccgggtttat cgatcaccgg ttccccgggc
     1381 gcgggataca gttccggtac gatgtcgtgg ccgatttctc cgcgaaccag gatgcgcccc
     1441 atcggtccgg gatcgccgat gcgcgtcttg cccctgcccc gcaccagctt ggttggcggg
     1501 cagtcggtca ggtccggccg atggccctcg cgggtgtgga tgaccagcat ccctcgcttc
     1561 cgcgccgact ccagcacccg cttggtcggg gcgatggtcg cctgcagcgg cgtgacgtcg
     1621 ttgcccagtg cctcgccgaa tccgccgggc atgacgaaat cgcgctgcat gtcgatgatc
     1681 agcagcgccg tggtcttcgg atcgaactcc agttcgtacg gttcagcgtc gatcgccttg
     1741 tttgccattt ttaccctcct cactgatccg tctgcatggc agggcgctac cggtgaaccc
     1801 gatgaggaaa ccgcgccgcg cccgctttgc gctcccttgg tatactcact agtatacaag
     1861 atgcaacacg ggagaaagca gtgtccatga ccatatcttc gttgctcgcc gattaccggt
     1921 cgggaaagcg tacacccggc gcagtagtgg aatcactggt atccggggac gatgaaacct
     1981 atgcctgcgc ctggatttgc aaggtcgggc cggacgtgct tgcccgccgc gcccgccaac
     2041 tcgaacaggc gctgcgcgag gacccgcgcg cgatcgaccg cctgccgctg ttcggggtcc
     2101 cgttcgcggt caaagacaac atcgacgttg ccggtctgcc gaccaccgcg gcctgtcccg
     2161 ccttttccta cgttcccgag cgctccgcca cagcggtgga gaaactcgag gcggccggcg
     2221 ccatcctggt cggcaagacc aatctggacc agttcgccac cggactggtc ggcacgcgct
     2281 caccctacgg cgccgtgccc aacgcgttcc ggcccgagta tatctccggc ggctccagtt
     2341 ccggctccgc ggtcactgtc gcccgcggcc aggtaagctt tacgctcggg accgacactg
     2401 cggggtcggg ccgggtgcct gccggattca acaacatcgt cggtttgaaa cccagccgcg
     2461 gcctgatcag cgcacgcggc gtggtacctg cctgccagtc actcgactgc gtgtcggtgt
     2521 tcgcgcttac cgtgcccgac gcggtggcgg tactcggtgt ggcgcgcggc ccggatccgg
     2581 aagatccatg gtcgcgcacc ctgaatcttt ccgtgccacc gctggccgag cgcttcacgt
     2641 tcgcgctacc cggaccgctc gaattttatg gcgacgacca cgccaggcac gcgttccacg
     2701 acgcggtcga caagctcgaa ggcctcggcg gcaaaccaat cggcgtcgat ttctcggtgt
     2761 tcctggaaac cgctgcattg ctctacgaag gaccctgggt ggcggagcgg ctggcagcga
     2821 tccggccgtt cttcgaaagc catcccgatc aggtgcaccc ggtggtacgc aagatcatcg
     2881 agggggcatc cggattctcg gccgcggact tgttttcagg cttgaccaag ctggaggcgc
     2941 tgagaaagcg cgcagctgga ctctgggacc gggccgacgt gctggtggtg ccgacctcac
     3001 ccaccatcta caccatcaag gaagtgctcg cggaaccgtt cctgaacaac cgcaggctcg
     3061 ggcactacac taatttcgtg aacctcatgg acctagccgc ctgcgcggtt ccggcgtcga
     3121 tgcgcccgga tggcctgcct tgtggcatca cgctgattgc gccggctgga accgatctca
     3181 tgctcgcgaa tctggcgcag cgctttcatc atctcaccgg gctcaagctc ggcgcgctgg
     3241 acgaagtgct gccgccgccc gcggcaatcg cgaccggcga cgacagcgtg cgcgtggccg
     3301 tggtcggggc gcacctgtcg ggactgccgc tcaaccatca attgaccggg cgcggagcgc
     3361 ggctggaaaa accggcgcgc accaccgcgc gctaccggct gtttgcgttg cccggcacta
     3421 cgccgccgaa acccggaatg gttcgcgatg ccgacaaagg cagcgtcatc gatgtcgagg
     3481 tgtggcggct gcccgtagcc gaatacggct cgttcgtagc cgggattccg tcgccactgg
     3541 gaatcggaca agtcgaactc gatgacggcg agtgggttca gggtttcctg tgcgagagct
     3601 gggcggtgaa cggcgccgag gagatctcgc acctcggcgg ctggcgcgca tatctcgcga
     3661 gcaaggctgc gcgttgaagc ggaccggaaa tcgcggccgg tcggcgaccg tcaggtcgac
     3721 cgccggcaag cgccggaagg tgacgacgga tgaggaacgc aagccgtcgc ttgccgagtc
     3781 ggtgtatctg ggcatcaagc aagacatctt cgatttccgc ctgctgcccg gggatcggtt
     3841 taccgagaac gaagtcgccg ggcggctgaa cgtcagccgc accccggcgc gcgaagcgct
     3901 ttaccgcctg gcgcgcgagg gctacctgaa agtgcactat cgcagcggct ggagcgttcg
     3961 cgaactcgat ttcaagcagt tcgacgatct gtacgacctg cgcatcgcgc tcgagaccgc
     4021 cgcggtgaaa cagctctgcg atgaagccgg gcgccccaac ctggaggacc ttaaatctat
     4081 ctggctggtc cagccggaaa cgagactcga cgacggggag cgggtgtgcg ccctggatga
     4141 agcctttcac tgcgcgctgg tcgccgccac aggcaatcgc gagatggcac gctgccacca
     4201 ggacgtgacc gagcgcatcc acttgctgcg ccggctcgat ttcaccgaag ccggccgtat
     4261 cgccgccacg taccaggagc atgcgcagat tctgcgcgcg gtgctgcggc aggatgctga
     4321 tcgcgcaatc gggttattgc aggctcacat cgggaccagc aagatcgaag tccgaaaaat
     4381 cagcctgcac aagcttttgt cggcaaggcg tgcctagtgc tactcccggt gcgcaccggc
     4441 cttgattgaa gtgccggaaa attgtatcag tgcaactgga cgacgtttga tgggatgagt
     4501 ggcgcgatcg gaagattcag cgccagcaat tcccggccca gcagcgtgat gctgcccttg
     4561 atgcggttgt cgccggtatc gctgaattcg aactcgtaca cccgcttcac gcgcacgcgg
     4621 ccctcgtcgt cacggccgag ccgcgtctgc ttcagcatta ctgtccaatc gagcaactgc
     4681 acgccttccg cggcgcaggc gcggcggccg gcgtcgagcg ccgcttcgcg ggcacgcatg
     4741 ctgtcggtcc agaaccaggc gccggcaacg aaaattatca aagtggcaag ttcgatcatt
     4801 gggatgtcaa tggcagaggc tagaggctag aggctagagg ctagaggcta gaggctagga
     4861 aaggctagtc gatctttacg cagcacgcgc ttttgctcct cactcctctc gcctcacgtc
     4921 cctttccatc gctcaggacc tgccggtgag aatctggtag agcgtgtaca gaatccccgc
     4981 ggtggcgccc cagatgtagc gatccccgta agccatcgaa tagaagtggc gggtgcgccc
     5041 gttgaataca tcgctgtggc gttgatggtt caccgggtcg agaaagaatt ccagcggcac
     5101 ttcgaatacg tcggccactt cgaatggatc gggactcaga tcgaacggag gctccaccca
     5161 cccgacgatc ggtgtcaccc ggaatccagt caggatatcg tagtcgggca ggcgcccgat
     5221 gatctcgacg tgctgccggc tcagtccggt ttcctcctcg gcttcgcgca atgcggtggc
     5281 gatccggtcg ggatcgccct catcgacgcg tccgccggga aagctgatct gcccggcatg
     5341 gtcgtgcaga tgggctgtgc gctgggtgaa aagcagggtc ggcccggctg actggttcac
     5401 caccggcacc agtaccgcag ccggaaccgg ctcgcgctct gggggatggg cgtaacgggg
     5461 aaaattggcg tcctccggca agatgccctg ccggctaaga cgctccgcca gccattcccg
     5521 ggtaaagggc ggcggcttca agtcatcaga aggagacaac ggtctgcaat gcgctagtaa
     5581 tggaggaggc tattttatcc ttcacgcaat ggtatagaat ctccgggcac cccggaacgg
     5641 cgacaattga ccgctgtcga agggcgcggc caggaactgc cgaaacacga tacgaggaga
     5701 cgacgatgaa actgcgcagt ctgattgctg ccgtaatgtt cgcaatggca ggtagcgcgt
     5761 ttgcgatgca ctgccctatg gacatgaaga aaatcgacga agcattggcg aagaagccgg
     5821 aaatctccgc cagcaagctc gcggaggtaa agaagctgcg ggccgaaggc gagaagttgc
     5881 acaacgccgg caagcaccag gagtcggtgg acacccttgc caaggcgatg gcgattctgg
     5941 gaatcaaata accagaaaca tgcacaccag ccccgctccg cggcgacgcg cagcggggtt
     6001 ttttgcgacc gtcacgccgt cacgatcgta acgtcgtcac ggcctttagg tcacggcacc
     6061 gccgcaggat gagccggatc cagcggtgca gccgtagcag tgattcgcgg tgacaatgcg
     6121 tctcccgttc agttttgcaa gatcgaagtc gcgaatatgc ctcggcgttc cgttggcaag
     6181 cggcaagtcc aacatctggt tgaagtcgca gtcatagagc gtgccctccc agccaaccga
     6241 tagagtgtag cggcacatca cgcccgccgc cgccgcggga ttgaacgcgt tcgccaggcg
     6301 ctccatgtag ccctcgtagt ttccgctctc gatgaggaat tc
//
LOCUS       DASQOP010000082         4229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1240673, whole genome shotgun sequence.
ACCESSION   DASQOP010000082 DASQOP010000000
VERSION     DASQOP010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240673"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VE030_03225"
     CDS             complement(<1..239)
                     /locus_tag="VE030_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HZF22460.1"
                     /translation="MANTHSNVRFVSLITKNTYGLVLAGGRGSRLRNLTDWRTKPAVP
                     FGGKFRIIDFPLSNCVNSGIRRIGIATQYKSHSLIR"
     gene            428..2614
                     /gene="glgB"
                     /locus_tag="VE030_03230"
     CDS             428..2614
                     /gene="glgB"
                     /locus_tag="VE030_03230"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HZF22461.1"
                     /translation="MKAPVSAIAGADLDALVGARHHDPFGVLGLHRDGKGWVLRVFRP
                     YASEVAASTASGFVKMDKVHPDGVFAWRGAEPPPQPVRLRVKEPGHSLETCDPYGFRP
                     TISADELYLFNEGRLEQAYRMLGSRTEVREGVAGVRFACWAPNAERVSVVGDFNRWDG
                     RAHPMATHGVSGVWELFVPGLGDGTLYKYEIRNRNTGEILVKTDPYARAYELRPGTAA
                     RVTADLPYDWRDGGWMKARAGDDWLHAPVSAYEVHLGSWRRHPDGRFYSYRELAESLV
                     PYVKEMGFTHVELLPVSEHPLDESWGYQTTGYFAATSRFGSPEDFKFLVDACHQAGIG
                     VILDWVPAHFPTDAFALARFDGTALYEHEDPRLGFHQDWGTHIFNYGRNEVKSFLLSS
                     AHYWLGECHVDGLRVDAVASMLYLDYSRKTGEWIPNKFGGRENLEAIDFLRQLNVMVH
                     RDFAGALTFAEESTAWPMVSRPTYIGGLGFSMKWNMGWMNDTLSYMRQDPVHRRYHHN
                     QLTFGQLYAYTENFMLPLSHDEVVHGKGSLLGKMPGDAWQKFANVRLLLSYQFASPGK
                     KLNFMGNELAQGREWRTEWELDWGLLGIDWHRGVQTMLRDLSRLYREQRALHDQDFES
                     AGFRWIDCHDADQSILAFERCARDGSVAVVALNFTPVPRHGYRIGLPKAGVWHEAFNS
                     DSAFYAGANVGNSGAIETQPLPWMERDQSAEITLPPLAALILVHQG"
     gene            2624..4090
                     /gene="glgA"
                     /locus_tag="VE030_03235"
     CDS             2624..4090
                     /gene="glgA"
                     /locus_tag="VE030_03235"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HZF22462.1"
                     /translation="MPPMPDGKLKVLFATSEVAPLIKTGGLADVSAALPAALMGLGAD
                     MRVLVPGYPQVMEALKTKGRAATLPALPGVPPAQLIASKLPSGVPLLVIESAIYQRSG
                     GPYQDAAGNDWLDNDLRFGLLSYVGALLSTPDSPFPWKPDIVHCNDWQTGLTPVYLHY
                     VPGERAKTVITIHNLAFQGIFPAETAARLGLPPQAFSVNGVEFYGKTSFLKGGLQFAD
                     RITTVSPSYAEEILGEPLGMGLQGLLSHRKDVLIGILNGIDTDAWDPESDPYIERYYN
                     ASRLAAKQDNKRALQLRMGLRDEPEMPLFGTVSRLTHQKGHDVLADIATELVKLPAQL
                     VVLGSGDAQLQLRFQELAQSHPGRIAVQISFDEGLSHQIEAGADSFVMPSRFEPCGLN
                     QMYSQRYGTPPVAHATGGLRDSVVDCTPETLKNHTATGFMFAPLNRDALLEACQRAVA
                     AYHDKRIWRQLQKNGMSRDFSWEARAQQYLDLYRGLLE"
BASE COUNT          758 a         1332 c         1380 g          759 t
ORIGIN      
        1 cggatcaggc tgtgcgactt gtactgggtg gcgatgccga tgcggcggat gccggagttg
       61 acgcagttcg acagcgggaa gtcgatgatg cggaacttgc cgccgaaggg caccgccggt
      121 ttcgtccgcc agtccgtgag attcctcaaa cggctgccgc gtccgccggc aagcaccagt
      181 ccataggtgt tcttggtgat gaggctgacg aagcggacgt tcgagtgagt gttggccacg
      241 cgttgctcct ggtgcacacg ttgaaggatc ggaagcgccg gacgcgcggg cttcgcgctt
      301 gccataaacc tttgaccggt atcaaaaagc agaggagcgg aaggccccgg cccttataat
      361 aggtgcaccc tcgtccgcag tcacgatttc cgtgcattgc ccggtcgaga gtttcccgcc
      421 accgctcatg aaagcacctg tttccgccat cgccggagcg gacctcgacg ccctggtcgg
      481 cgcacgccat cacgatccgt tcggcgtgct cggcctgcat cgcgacggca agggctgggt
      541 gttgcgcgtg ttccggccct acgcctccga ggttgccgcc tccaccgctt ccggattcgt
      601 gaagatggac aaggtgcatc ccgacggcgt gttcgcatgg cgcggagccg agccgccgcc
      661 tcagccggtg cggttgcgcg tgaaagagcc ggggcacagc ctggaaacct gcgacccgta
      721 tggttttcgt cccaccatca gcgccgacga actgtacctg ttcaacgaag gccgacttga
      781 gcaggcgtac cgcatgctcg gcagccgcac cgaggtgcgc gagggtgtgg ccggggtgcg
      841 ttttgcctgc tgggcgccga atgcggaacg ggtcagcgtg gtcggcgact tcaatcgctg
      901 ggacgggcgc gcgcacccga tggcgacgca cggggtgagc ggcgtctggg aactgttcgt
      961 cccgggattg ggcgacggca cgctctacaa gtacgagatc cgcaaccgta acacgggcga
     1021 aatcctcgtc aagaccgatc cctacgcacg cgcctatgaa ctgcgccccg ggaccgccgc
     1081 gcgcgtcacc gcggatcttc cctacgactg gcgggacggc gggtggatga aggcgcgcgc
     1141 cggcgacgat tggctgcacg caccggtcag cgcctacgaa gtccacctgg gctcgtggcg
     1201 gcgccatccc gacgggcgct tctacagcta ccgcgagctt gccgaaagcc tggttcctta
     1261 cgtaaaagaa atgggtttca cccacgtcga attgctgccg gtttccgaac atccgctgga
     1321 cgagtcctgg ggttaccaga ccaccggtta tttcgcggcg accagccgct tcggctcgcc
     1381 ggaggacttc aagttcctcg tggatgcgtg ccaccaggcc ggcattggcg tgatcctcga
     1441 ttgggtgccg gcgcatttcc cgaccgatgc gttcgcgctg gcgcgtttcg acggcaccgc
     1501 gctttacgag cacgaggatc cgcgtctcgg ctttcaccag gactggggca cgcacatctt
     1561 caactacggc cgcaacgagg tgaaaagttt cctgctgtcg agcgcgcact actggctggg
     1621 cgagtgccac gtggacgggc tgcgggtaga cgcggtggcc tcgatgctct atctcgatta
     1681 ctcgcgcaag accggcgagt ggatccccaa caaattcggc ggccgcgaga acctcgaagc
     1741 gatcgatttt ctgcgtcagc tgaacgtcat ggtccatcgc gactttgcgg gcgcgctcac
     1801 tttcgccgag gaatccaccg catggccgat ggtgtcgcgg ccgacctaca tcgggggact
     1861 gggcttctcg atgaagtgga acatgggatg gatgaacgac acgctttcct acatgcgcca
     1921 ggacccggtg caccggcgtt atcaccacaa ccagctcacc ttcggccagc tctacgccta
     1981 caccgagaac ttcatgctgc cgctgtcgca cgacgaagtg gtgcacggca agggttcgct
     2041 gctgggcaag atgcccgggg acgcctggca gaaattcgcc aacgtgcgcc tgctgctcag
     2101 ctaccaattc gcctcacccg gcaagaagct gaacttcatg ggcaacgaac tggcacaggg
     2161 ccgggaatgg cgcaccgagt gggagctcga ctggggactg ctcggcattg actggcaccg
     2221 cggcgtgcag acgatgctgc gcgacctgtc gcgcctttac cgcgagcagc gcgcgctgca
     2281 tgaccaggac ttcgaatccg ccggtttccg ctggatcgac tgtcacgatg ccgatcagtc
     2341 cattctcgct ttcgaacgct gcgcgcgcga cggctcggtg gcggtggtgg cgctgaattt
     2401 caccccggtg ccccgccatg gctaccgcat cggcctgccg aaggccggcg tctggcacga
     2461 agcgttcaac agcgattcgg cgttttacgc cggtgccaat gtcggcaatt cgggcgccat
     2521 tgagacccag ccgctgccgt ggatggagcg cgaccagtcc gcggaaatca cgctgccccc
     2581 gcttgccgcg ctcatccttg tgcaccaggg atgatgcgcg taaatgccgc cgatgcctga
     2641 cgggaaactc aaagtcctgt tcgccacttc cgaagtcgcg ccgctgatca aaaccggcgg
     2701 gcttgccgac gtgagcgccg cgctgccggc ggctttgatg ggactgggcg cggacatgcg
     2761 ggtgctggtg ccgggatatc cgcaggtgat ggaggcgctc aagaccaagg gccgggcggc
     2821 caccttgccg gcgttgccgg gcgtgccgcc ggcgcaactg atcgcctcca agctgccttc
     2881 gggggtgccg ctgctggtga tcgaaagcgc gatctaccag cgtagcggcg ggccgtatca
     2941 ggatgccgcc ggcaacgact ggctcgacaa cgatctgcgt ttcggcctgc tttcgtacgt
     3001 cggcgcgctg ctgtccacgc cggactcgcc gtttccgtgg aagccggaca tcgttcactg
     3061 caacgactgg cagaccggcc tgaccccggt ttacctgcac tacgtaccag gtgagcgggc
     3121 gaaaacagtg atcaccatcc acaatctggc gttccagggg atcttccccg cggaaacggc
     3181 cgcgcggctt gggctgccgc cgcaggcgtt ctccgtgaac ggcgtcgaat tctatggcaa
     3241 gacgtcgttt ctcaaaggcg gactgcaatt cgccgatcgc atcaccacgg tcagtccgag
     3301 ctacgcggaa gaaatcctgg gtgaaccgct ggggatgggg ctgcaaggtc tgctctcgca
     3361 cagaaaggat gtgctcatcg gaattctcaa cggcatcgac accgatgcct gggacccgga
     3421 gtccgacccg tatatcgagc gttactacaa cgcctccagg ctcgccgcca agcaggacaa
     3481 caagcgcgcg ctgcaactgc gcatgggcct gcgcgacgag ccggaaatgc cgctgtttgg
     3541 cacggtgagc cggcttaccc accagaaagg ccacgacgtg ctcgccgaca ttgccaccga
     3601 gctggtgaag ctgccggcac aattggtggt gctgggctcg ggagacgcgc agttgcaact
     3661 gcgtttccag gaactggccc aatcgcaccc gggcaggatt gcggtgcaga tcagcttcga
     3721 cgagggtctg tcgcaccaga tcgaggccgg cgcggacagc ttcgtgatgc cgtcgcgctt
     3781 cgagccctgc ggcctgaacc agatgtacag ccagcgttat ggcacgccgc cggtagcgca
     3841 cgccaccggg ggcctgcgcg attcggtggt ggattgcacg ccggagacgc tgaagaatca
     3901 caccgcgacc ggtttcatgt tcgccccgct gaatcgcgac gcgttgctgg aagcgtgcca
     3961 gcgcgcggtc gctgcgtacc acgacaaaag gatatggcgg caattgcaga agaacggcat
     4021 gagccgcgat ttcagctggg aggccagggc gcagcagtat ctggacctct accgtgggtt
     4081 gctggagtaa aatgccattt tcctgcccgc ggcgctcgtt cgcgtttgcg gcgcaacaac
     4141 atcaacggac ggagggtcgc cagtcatgat cggagatcga attgaagtga acattgccgg
     4201 cgggctcgat atcgcgctgc caaggatgg
//
LOCUS       DASQOP010000083         2939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1240698, whole genome shotgun sequence.
ACCESSION   DASQOP010000083 DASQOP010000000
VERSION     DASQOP010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240698"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="VE030_03240"
     CDS             <1..969
                     /locus_tag="VE030_03240"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HZF22463.1"
                     /translation="IDQDPRQIEYLWELMHRRAYKHARGGIVIAAISGIDIALWDLRG
                     KMASMPLWQLLGGYRKRVPAYATGGFYAEGKGVRELVKEMESYVKHGFRAVKMKVGRN
                     AGIELSPLRVMDNRGQCEVTLAQDLERVAAVRDAIGPDIKLMVDANGAWDVATAVKMG
                     RAMERHDIYWYEEPVWPDDVAGSAEVAAKVGIAIAGYETCSYGIVDFNDYIKARAVHF
                     VQPDVAWAGGLTESLKIAHMAQAANLPIAPHIHGSAVAVAAAVHFLGAVRNGSMAETV
                     YPAHALMTELVKEPFAVDKKGDIELPDRPGLGVELDPRIVKKYLAR"
     gene            1335..2252
                     /locus_tag="VE030_03245"
     CDS             1335..2252
                     /locus_tag="VE030_03245"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZF22464.1"
                     /translation="MTDAIIYLATSFWIGAVHAATPGHGKTIAAAYIVGARGKPVDAV
                     ILGVFVTLSHTSGIVLVGVLASLGLPGMMPQRIEAWMALVTGLLVIGIGLWTLWTQRE
                     LMLQLQGVAPAPWHAHDHAHENAQARAEMRFRPHAHDGERGHGHHQHGHDHAHGHGHS
                     HDHDHAHGHAHPHYDAESGSHSHGWGFKHTHDLKLATTNRPSMWVLIGLGIAGGLLPD
                     PAALAILLNALAQGKVMLGLGTVVVFSIGFASTLVIVGAIAAKVGQKILDWLAGPWAA
                     RLQISTSLLIVLVGVILSFKAWTTLSTLA"
     gene            complement(2399..>2939)
                     /locus_tag="VE030_03250"
     CDS             complement(2399..>2939)
                     /locus_tag="VE030_03250"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HZF22465.1"
                     /translation="TGYGFTRLKDGVLARNVPKSVRGGPYGVAVDAQGNLWGTLQSGN
                     QIVKISPAGEIAEYEVPTRASAPSDIVVDGAGNTWFLEFRSNKIGKFAGGKFAEFEVP
                     GEWGGLTGLTAAPDGSVWFGLLRGHRLGRLHEGEMKMFPLPRADARPYTLAADKKGNI
                     WYADISGYVGMLKADAAKR"
BASE COUNT          550 a          933 c          905 g          551 t
ORIGIN      
        1 atagaccagg atccgcggca gatcgaatac ctatgggagt tgatgcaccg gcgcgcgtac
       61 aagcatgcgc gcggcggcat cgtcatcgcc gcgatcagcg gaatcgacat cgcactctgg
      121 gacctgcgcg gcaagatggc cagcatgccg ttgtggcagt tgctcggcgg ctaccgcaag
      181 cgcgtcccag cgtatgcgac cggcggtttt tatgccgagg gcaagggcgt gcgcgaactc
      241 gtgaaggaga tggagtccta cgtcaagcat ggtttccgcg cggtgaaaat gaaagtcgga
      301 cgcaatgccg gaatcgagtt gtcgccgttg cgtgtcatgg ataaccgcgg ccaatgcgaa
      361 gtcacgctgg cgcaggatct cgagcgcgtc gcagcggtgc gcgacgccat cggtccggac
      421 atcaagctga tggtggacgc caacggcgcc tgggatgtcg ccaccgcggt gaagatgggc
      481 cgtgcgatgg agcgccacga catctactgg tacgaagagc cggtgtggcc ggacgacgtc
      541 gccggcagcg ccgaggtcgc cgccaaggtc ggcatcgcaa ttgcgggcta tgaaacctgc
      601 agctacggca ttgtcgattt caacgactac atcaaggcgc gcgcggtcca tttcgtgcaa
      661 ccggacgtgg cgtgggcagg cgggctcacc gaatcgctga agatcgcgca catggcgcag
      721 gccgcgaacc tgccgatcgc gccgcacatt cacggctcgg cggtggcggt cgccgctgcg
      781 gtgcattttc tgggtgcagt caggaacggc tcgatggcgg aaactgtcta tcccgcgcac
      841 gcgctgatga ccgagctggt caaggagcct ttcgctgtgg acaagaaagg cgatatcgaa
      901 ttgcccgacc gccccggact gggcgtcgaa ctcgatccca ggatcgtgaa gaagtatctc
      961 gctcgttaga agacgagatc aagagcaact ctcaccacgg aggacacgga ggaaacccaa
     1021 aaacgaggaa aggcaaactg gaggcaaagg ccgactggta ttcgtggacc actaccgcta
     1081 accttcacag cgatgggtca tcaacaattg aaaggttttt accatggccc acttttttac
     1141 tcctgctttt cctccgtgtc ctccgcgtcc tccgtggtaa gcgctgttct ttgggtctcc
     1201 gtggtgagcg ctgttctttg ggtctccggc gatttcatcc gtcgcacgcg cgcgccttga
     1261 cagggcagag ggcggcacct attctcgggc ccggacaacg ataacaacct ccccgcagta
     1321 ccgattcgcc tcccatgacc gacgcaatca tctatctggc caccagtttc tggatcggcg
     1381 cggtgcacgc cgccacgccg ggccacggca aaaccatcgc cgccgcttat atcgtcggcg
     1441 cgcgcggcaa gccggtggac gcagtgatcc tgggtgtgtt cgtcacgctg tcgcacacca
     1501 gcggcatcgt gctggtgggc gtattggctt cgctcggact gccgggaatg atgccgcagc
     1561 gcatcgaggc ctggatggcg ctggttaccg gtctgctggt gatcggcatc ggtctgtgga
     1621 cgttatggac gcaacgggaa ttgatgctgc agctgcaggg cgtcgcacct gctccgtggc
     1681 acgcgcacga tcacgctcat gaaaatgcgc aagcgcgcgc cgaaatgcgt ttccgcccgc
     1741 acgcccacga tggcgagcgc ggacatggcc accatcagca tggccatgat cacgcacacg
     1801 ggcacggtca ttcccacgac catgaccacg cgcacggcca cgcgcatccg cattacgacg
     1861 cggaatcggg ctcgcacagc cacggctggg gattcaagca cacgcacgac ctgaagctcg
     1921 ccaccaccaa ccgccccagc atgtgggtgt tgatcggact aggcatcgcc ggcggcctgc
     1981 tgcccgaccc ggcggcgctc gcgatcctgc tgaacgcgct ggctcagggc aaggtgatgc
     2041 tgggcctggg aaccgtggtg gtattcagca tcggcttcgc atccacgctg gtgatcgtcg
     2101 gcgcgatcgc cgcgaaagtc gggcagaaga tcctcgactg gctggcggga ccatgggccg
     2161 cgcggctgca gatttcgact tcgctgctga tcgtgctggt cggcgtgatt ctttccttca
     2221 aggcctggac cacgctcagc acgctcgcgt gagacatgct tcgcagggaa gagtgatgag
     2281 ggatgagtaa aaaccaattt tcctagtccc tggctcctaa tccctcgccc ctgtttgtct
     2341 ttacccttca ctcattcacc cttcacgcat tcactgccgt taggcccgtc cctagtccct
     2401 atcttttcgc cgcgtccgcc ttcagcattc ccacgtagcc gctgatatcg gcgtaccaga
     2461 tatttccttt cttgtcggcc gccagcgtgt agggccgcgc atcggcgcgc ggcaatggaa
     2521 acatcttcat ctcgccctcg tgcaagcgcc caaggcggtg cccccgcagc agtccgaacc
     2581 agacagaacc gtccggcgcg gcggtaaggc ctgtcagccc gccccactct ccaggcacct
     2641 cgaattcggc gaacttgcct ccagcgaact tgccgatctt gttgctgcgg aactcgagga
     2701 accaggtgtt gccggcgccg tccacgacaa tgtcgctggg ggcactggca cgcgtcggca
     2761 cttcatattc ggcgatctct cccgccggag agatcttcac gatctggttg ccgctttgaa
     2821 gcgtacccca cagattgcct tgcgcatcca cggcaacgcc gtagggcccg ccgcgaaccg
     2881 acttcggcac gttgcgggcc aacacgccgt ccttgagacg ggtaaagccg tagccggtg
//
LOCUS       DASQOP010000084         2853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_633704, whole genome shotgun sequence.
ACCESSION   DASQOP010000084 DASQOP010000000
VERSION     DASQOP010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633704"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..776
                     /gene="orn"
                     /locus_tag="VE030_03255"
     CDS             231..776
                     /gene="orn"
                     /locus_tag="VE030_03255"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HZF22466.1"
                     /translation="MAQDPSYLIWIDMEMSGLNPDADRVLEVAIVVTDSQLVTVAEAP
                     VKVVHQADAVLDRMDSWNRSTHKKTGLTDKVKAARQNEAEVEAELIAFLAQYLPENTS
                     PMCGNSICQDRRFLARYMPKLEAFFHYRNLDVSTLKELAKRWKPGIVAGMTKHGKHEA
                     LADIYESIEELKYYRENLLNL"
     gene            complement(911..1117)
                     /locus_tag="VE030_03260"
     CDS             complement(911..1117)
                     /locus_tag="VE030_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6494 family protein"
                     /protein_id="HZF22467.1"
                     /translation="MNEENFNMSIRKFLKMTGVGSQREIEQAVSKALASGKLKGHEKL
                     PATINLTIPAIGLNVDFDGEITLE"
     gene            complement(1114..2775)
                     /gene="pgi"
                     /locus_tag="VE030_03265"
     CDS             complement(1114..2775)
                     /gene="pgi"
                     /locus_tag="VE030_03265"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017332440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HZF22468.1"
                     /translation="MPRLHQRPAWKALEAHAQEVREVRVADLFAQDPARAQEFSIAAC
                     GLLFDYSKNPLSRQTCRLLVALAEDASLPAQITAMFEGKRINVTEKRAVLHTALRNRS
                     GRPVLLDGKDVMPDVRAVLSRLRDFSERVRGGIARGYTGRPFADVVNIGIGGSDLGPL
                     MACSALRPYCTPDLRVHFVSNVDGAHLAATLSDLDAATTLFVVSSKTFTTQETLTNAC
                     TAREWLLTRLHPSGAAEAAVQKHFAAVSTNLEATSQFGIAPERVFGFWDWVGGRYSLW
                     SAIGLPIALAIGFDGFDELLAGAYEMDEHFRTAPLERNMPAIAALTGVWHINFLGHPT
                     HAVLPYAQDLAHFPAYLQQLDMESNGKRVDRSGAAVEYATGPVIWGAPGTNGQHAFFQ
                     HLHQGTQVTPADFIVAANGAERMPEHHDILLANCIAQSEALLRGKNADEVRAELKARG
                     IEGDEAERLVAHRAFPGNRPSNTIVLRQLDPRSLGALIALYEHKVFAQGAVWNINSFD
                     QWGVELGKQLANGVLEDLRAGKARSGHDASTSRLIDYVLAHKEDK"
BASE COUNT          561 a          854 c          906 g          532 t
ORIGIN      
        1 ggtgcgcttc gaggctgatt tcctcggcaa acgcagcggg gaccgcatcg cggtgcgccc
       61 gtatatgctg gacatgccgc gttgccagcc acaggcgcag gccggtagcg atcagcagcg
      121 cggcgagaaa cagaacagag aacgtggaca ttgcggcttt cgactaggga ttcagtgcaa
      181 gctatgacaa aatatcgtca ggaaaattca ggaaaaactc accgaggatt atggcacagg
      241 atcccagtta tctgatctgg atcgacatgg aaatgagcgg gctgaacccg gacgccgacc
      301 gtgtcctgga ggtcgcgatc gtcgtcaccg actcgcaact cgtcaccgtg gccgaagcgc
      361 cggtgaaggt cgtgcaccag gcggacgcgg tgctcgatcg gatggattca tggaacaggt
      421 ccacgcacaa gaaaaccgga ttgaccgaca aagtgaaggc ggccaggcag aacgaggcgg
      481 aagtggaggc cgaactgatc gccttccttg cccagtacct gcccgagaat acctcgccga
      541 tgtgcgggaa ttcaatctgc caggaccgtc gctttctcgc caggtacatg ccgaagctcg
      601 aagccttctt ccactaccgc aacctcgatg tttccacgct caaggaactg gcgaagcgct
      661 ggaagccggg aatcgtggct ggcatgacca agcatggcaa gcacgaagcg ctggccgaca
      721 tctacgaatc tatcgaggag ttgaagtatt accgcgagaa tttgttgaac ctgtaattgc
      781 ggtagggcgt gaggggtgag gagtgaggcg caacccgcat atgcaaaaaa cttccactct
      841 ttgaaaagaa ctacgctcta ctcgcctctc gcctctcgcc tctcgcctct cgcctctcac
      901 ccttcaccct tcactccaga gttatctccc cgtcgaagtc gacgttcagc ccgatcgcgg
      961 gaatggtgag attgatcgtc gccggcagtt tctcgtggcc cttcaatttt cccgaggcca
     1021 gcgccttcga caccgcctgc tcgatctcgc gttgcgagcc cacgccggtc atcttcagaa
     1081 acttgcggat gctcatgttg aaattttctt cgttcatttg tcctccttgt gtgcgagtac
     1141 gtaatcgatc agccgcgagg tagaggcatc gtggccgctg cgggctttcc ccgctctcag
     1201 gtcttcgagc acgccgttgg cgagctgctt gccgagttcc acgccccact gatcgaagga
     1261 attgatgttc cagaccgcac cttgcgcgaa caccttgtgt tcgtacagcg caatcagcgc
     1321 gcccagcgag cgcgggtcca gttgacgcag cacgatggta ttgctgggac ggttgccggg
     1381 gaaagcgcga tgcgcaacca ggcgttcggc ttcgtcgccc tcgatgccgc gcgccttgag
     1441 ctcggcgcgg acctcatcgg catttttccc ccgcagcagc gcctcgctct gggcgatgca
     1501 gttggccagc aggatgtcgt gatgttccgg catgcgctct gcgccgtttg ccgcgacgat
     1561 gaaatcggca ggcgtgacct gcgtgccctg gtgcaagtgc tgaaaaaagg cgtgctggcc
     1621 gttggttccg ggcgcgcccc agatcaccgg gccggtggcg tactcgaccg ccgctcccga
     1681 gcggtcgaca cgcttgccgt tgctctccat gtcgagctgt tgcaggtagg ccggaaagtg
     1741 tgcgaggtcc tgcgcgtagg gcaacaccgc gtgggtcggg tggccgagga aattgatatg
     1801 ccagacgccg gtgagcgcgg caatcgccgg catgttgcgt tccagaggtg ccgtgcggaa
     1861 gtgctcatcc atttcgtagg cgccggcgag cagttcgtcg aagccgtcga aaccgatcgc
     1921 caacgcgatc ggcaggccga tggccgacca cagcgaatat cggcctccga cccagtccca
     1981 gaagccgaat acccgctccg gggcgatgcc aaattgtgaa gtggcttcca ggttggtgga
     2041 gaccgcggcg aaatgcttct ggacggcagc ctctgcggcc ccggatggat gcagtcgagt
     2101 aagcaaccac tcgcgcgccg tacatgcatt ggtaagggtt tcctgtgtgg taaaggtctt
     2161 cgaggagacc acgaacaggg tcgttgccgc gtccagatcg gagagcgtcg cggccaagtg
     2221 ggcgccgtcc acgttggata cgaaatggac gcgcaggtcg ggcgtgcaat agggccgtag
     2281 ggccgaacag gccatcagcg ggcccagatc cgagccgccg atgccgatgt tgaccacgtc
     2341 ggcaaaaggc cggccggtgt agccccgcgc gatgccgccg cgcacccgtt ccgaaaaatc
     2401 ccgcagccgc gacagcaccg cccgcacgtc gggcatcaca tccttgccgt cgagcaggac
     2461 cgggcggccg gatcggttgc gcaatgccgt atgcagcacc gcgcgcttct cggtgacgtt
     2521 gatgcgcttg ccttcgaaca ttgccgtgat ctgcgcgggc aggctggcgt cctcggcaag
     2581 cgccactagc aggcggcacg tctgcctgga tagcgggttc ttcgagtaat cgaacagcag
     2641 cccgcaggcg gcaatcgaga attcctgcgc gcgcgcggga tcctgggcga acaaatcggc
     2701 aacgcgcacc tcgcgcacct cttgggcatg ggcctctagt gctttccagg caggacgttg
     2761 gtgcaggcga ggcatgggaa agaggcgagg ggcgaggggg cggatgaagg aatctggttt
     2821 taaccggatt ctataggacg gccgggaaat acc
//
LOCUS       DASQOP010000085         3269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_633949, whole genome shotgun sequence.
ACCESSION   DASQOP010000085 DASQOP010000000
VERSION     DASQOP010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633949"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1933
                     /locus_tag="VE030_03270"
     CDS             <1..1933
                     /locus_tag="VE030_03270"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZF22469.1"
                     /translation="VRFRHAGAQNVTARVSAEGDELPSNDALTEDLMVQPRLHALYVE
                     GGERGARYLADALTAQGIRVSVANAQNLSEDARLLDGKDVVILSDVRADSLGADVVQR
                     LRAFVRDRGRGLIFVAGQNTYGQDGFAKSKVEELLPVTFEARRKHEDLDLVLLVDRSS
                     SMRGQKIEVSKSAALATLDLLDPEHRLAVVAFDAQPHDVVPLMTVGNKLEAENLISRM
                     TSSGQTNIYNALLRAQALLAESKAKTKHIILLSDGLTSPPPGHVPASSRGGNEIEERI
                     RAIQTAERRQRGGAGEPPQAPQATASPRGFQGIMEELVDAKITMSTVAIGEKPDVELM
                     ANLANWGEGRSYVTRSDAEVPTLFAAETRRLLDDSIVEGRFRPRVKAWSPTLAGVDFA
                     GGPELKGYVTTRPKRFSDVLLEAKKDLPLLAETHYGLGKTVAFLSDVKNRWAADWLSW
                     PGYPALWAQVVRDSARRDSGEGLDWKVTREGREALIDLSGLGHDGSFRNGLWPDVRVT
                     APDGQRSVAALRQIAPGRYRARVPLTAAGNVPWRFDLLPVAGLSAADIARAGGRRLFY
                     SYPDEFRLLPANLPLLRTLSKETGGVFAPRAEEIFTPRGDGGVISTRLWPYCAAAALL
                     LFLLDILVRRAPWRLRRAP"
     gene            2289..>3269
                     /locus_tag="VE030_03275"
     CDS             2289..>3269
                     /locus_tag="VE030_03275"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZF22470.1"
                     /translation="MRTSIRQFLSLALPLAIAAAWAPGAQAAGSSASEIRNCSPENWC
                     FYHRTPDSSWRHSPLTQINAGNVKNLRPAWIFQPGNVRMGMHATPLAIDGNIYVSVNP
                     STVWKINGKTGERLWVHEPKMDEAVVARSFFAHTRGLAIGDGRVYMGQADGKIVALSE
                     KDGSTIWEKQIIDSRKDTVGFSGAATFVNSDLMVIGQNGGEYPIEGKIFGINPKTGDI
                     KWTFYTTGRDDARAMKTWRGDSWQYGGGGSWQPGTVDYANNQIIMGTGNPNPDYDYCG
                     SNCRDPNADGWRSGDALYTSSTVALDLNTGKLNWYFQEAHADPYDYDAAPG"
BASE COUNT          606 a         1033 c         1095 g          535 t
ORIGIN      
        1 cgtgcgcttc cggcacgctg gtgcccagaa cgtcacggcg cgagtgagcg ccgaaggcga
       61 cgagctgccg agcaacgacg cgctcaccga ggacctcatg gtgcagccgc gcctgcacgc
      121 gctgtatgtc gagggcggcg agcgcggcgc gcgctacctc gccgacgcgc tcaccgcgca
      181 gggcattcgc gtgtcggtcg ccaacgcgca aaacctgtcg gaagatgcgc ggttgctcga
      241 cggcaaggac gtcgtcatcc tcagcgacgt gcgcgcggac agcctggggg cggatgtggt
      301 acagcgactg agggcgttcg ttcgcgaccg tggccgcggc ctgatcttcg tagcggggca
      361 gaacacgtac ggccaggacg gcttcgccaa aagcaaggtc gaagagttgc tgccggtcac
      421 tttcgaggcc aggcgcaagc acgaggacct ggacctggtc cttctggtcg accgctcgtc
      481 gagcatgcgc ggccagaaaa tcgaggtttc gaagtccgcc gcgctcgcta cgctcgatct
      541 gctggacccg gagcacaggc tggcggtcgt ggcatttgat gcgcagccgc acgacgttgt
      601 cccactgatg acagtcggga acaagctcga ggccgagaac ctgatctcgc gcatgacctc
      661 gagcggccag accaacatct acaacgcgct gctgcgtgcg caggcgctgc tcgcggaatc
      721 gaaggccaag accaagcaca tcatactgct ctccgatggg cttacctcgc cgccgccggg
      781 ccatgtcccc gcatcctccc gcggcggcaa tgagatcgag gaaaggatcc gcgcgattca
      841 gaccgccgag agacgccagc ggggtggggc cggcgaaccg ccgcaggcgc cgcaggcaac
      901 ggcatcgccc cgtggtttcc agggcatcat ggaagaactg gtagacgcga agatcacgat
      961 gtcaaccgtg gcaatcggcg agaagcccga tgtcgagttg atggcgaacc tcgccaattg
     1021 gggcgagggc aggagctacg taaccaggag cgatgccgaa gtccccaccc tgttcgccgc
     1081 ggagacgcgc cgtctgctgg acgattcgat cgtcgagggg cggttccgcc cgcgggtgaa
     1141 ggcctggagc cctaccctcg ccggagtgga ctttgccggc ggccccgagc tcaagggcta
     1201 tgtgacgacc aggccgaagc gcttctccga cgtgctgctg gaggcgaaga aggatctgcc
     1261 cctgctggcc gaaacgcact acggcctcgg caagacggtt gcgttcctgt cggatgtgaa
     1321 gaaccgctgg gcggcggact ggctcagctg gcccggctac cccgcgctat gggcacaggt
     1381 cgtgcgcgac agcgcccggc gcgacagcgg tgaaggcctc gactggaagg tcactcgcga
     1441 ggggcgcgag gcactcatcg atctctccgg gctcggccac gacggcagct ttcgcaacgg
     1501 actctggccg gatgtgcgcg tcacggcgcc agacgggcaa cgttcggtcg ctgcactgcg
     1561 tcagatcgcg ccgggccgct atcgcgctcg cgtcccgctc accgccgcgg gcaacgtacc
     1621 ctggcgcttt gatctgctgc ccgttgcggg actgagcgcg gccgacatcg cgcgagccgg
     1681 cggccgcagg ctgttttatt cttacccgga cgaatttcgc ctgctgccgg caaacctgcc
     1741 gctcctgcgt accctgagca aggagactgg cggggtgttc gcgccccgtg cggaggagat
     1801 cttcacgcca cggggggacg gcggggtcat cagcacccgg ctgtggccgt attgcgctgc
     1861 cgcagccttg ctgctgttcc tgctggacat cctggtacgg cgcgccccgt ggcgcctgcg
     1921 ccgcgcgcct tgaccaaggg caagggcccg ccgccgggcg ggcgggggat cggtttgggc
     1981 agatgggtca accggacctt gggcaagact ctcaccttga cacccgttca gcgccgcaat
     2041 tagcatggac tgcgctggga ctttttcccg gctcacgcct taagcacgct ggacaatgtc
     2101 cgaaggggga gaagcttcca aatcgggttt gcgccacagg ttgagtgaag tactagctgt
     2161 gcaccctcag tggctctgtg ccaggcggtg gtgctgcagg cgtggctccc gagttccaat
     2221 ttacctctaa gatttaacag cttgttgttg gcgaccgatg ttcaaaccat aacgaggagg
     2281 agaaccagat gagaacgtca atacggcagt tcctgtcgtt ggcgctaccg ctcgccatcg
     2341 cggcggcgtg ggcgccgggc gcgcaggcgg ccggatcgtc ggcaagcgag atccgaaact
     2401 gctcgccaga gaactggtgc ttttatcacc gcacccccga tagttcgtgg cgtcacagcc
     2461 cgttaaccca gatcaacgcc ggcaacgtca agaaccttcg tccggcgtgg atcttccagc
     2521 ccggcaacgt gcgcatgggc atgcacgcca cgccactggc gatcgacgga aacatctacg
     2581 tatcggtgaa cccatcgacc gtctggaaga tcaacggcaa gaccggcgag cgcttgtggg
     2641 ttcatgagcc gaagatggac gaagccgtgg tcgcccgctc gttcttcgcg catacccgtg
     2701 gtctggccat cggtgatggc cgggtttaca tgggacaggc ggacggcaag atcgtcgctc
     2761 tcagcgagaa ggacggcagc acgatctggg agaagcagat catcgactcg cgcaaggaca
     2821 cggtcgggtt cagcggcgcc gcgacttttg tgaattccga tctgatggtg atcggccaga
     2881 acggcggcga atacccgatc gaaggcaaga tcttcggcat caaccccaag accggcgaca
     2941 tcaagtggac cttctacact acgggacggg acgatgccag ggcaatgaag acctggcgcg
     3001 gggattcgtg gcagtacggc ggtgggggat cctggcagcc ggggacggtg gactacgcca
     3061 acaatcagat catcatgggc accggcaacc cgaaccccga ctacgactat tgcggcagca
     3121 actgccgtga tccgaatgcc gatggctggc gctcgggtga cgcgctgtat acgtcgtcca
     3181 cggtcgcgct ggatctgaac accggaaagc tcaactggta cttccaggag gcgcacgccg
     3241 atccgtacga ctacgacgcc gcaccgggc
//
LOCUS       DASQOP010000086        12468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_232176, whole genome shotgun sequence.
ACCESSION   DASQOP010000086 DASQOP010000000
VERSION     DASQOP010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12468
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232176"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VE030_03280"
     CDS             complement(<1..184)
                     /locus_tag="VE030_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22471.1"
                     /translation="MKDEGESTAFEITSSFRLPPSSLLSLVPANAPAFVNLLVSEIGR
                     HSLPGDVGDVQPGERGG"
     gene            257..2338
                     /locus_tag="VE030_03285"
     CDS             257..2338
                     /locus_tag="VE030_03285"
                     /inference="COORDINATES: protein motif:HMM:NF021639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22472.1"
                     /translation="MADEPEIPASLREQVIAFFHSDAGSDHDAVELADRFATIAAAGN
                     LPDRLDALVRLIGWTHVADVDGGTGVPPERSRLFKFVQVIESVPPARRSVQDTFGEIL
                     SETEGVNLFGDSGIPSDRAFLAEFVTLVMRRLLPQPSDQHDLSRLVARLYSGRDAVER
                     LRTLAPEAFHRLLEPLTPPERPEIWAPLRTAFSDGFRLLAIRIEAQGLSAKLRARSHP
                     TPVANSPFHRIACTSHALMDAWKTGEDIAALLRSWRGQSAECREEIAEITRRLESAGV
                     SVDIVYGIEVLKRCLVRMEAMLEFIESPPGPARSAAIYRLLVGLIVAAHEDRSIRHLI
                     RVDLHLLQRKIVDRAGKTGEHYVARNLREYHGIWFAAAGGGLLTVLTAAVKLKVTHAG
                     LPLFVEGMLSGLNYAVSFMLLHHFHLMLATKQPAMTAATLATLLHSRDRAERLDRVVE
                     FTMRICSSQFAAAVANVIFVFLGAFVFSYIWNLALGRDYLGIKEAQQVFRTLSPVSSG
                     TVFYAALTGVILWMAAMIGGWFDNWAVYQRLPQAIADHRLGQRLGRKRMARVAGIVAR
                     NMAGWGTNISLGFLLGLTPVFGMFLGLPLDVRHVTLSSGMLAFACASLDGWFTAGWFV
                     WALAGVATMFVLNLSVSFFLSLYTAARAYELERRELVVLGMRLLRRLMSSPLDFVLPR
                     RFVESELKVES"
     gene            complement(2428..3192)
                     /locus_tag="VE030_03290"
     CDS             complement(2428..3192)
                     /locus_tag="VE030_03290"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HZF22473.1"
                     /translation="MNPRPLSPLVLACACLLAGCAVGPNPRDPYEAFNRKVFAFNEGL
                     DKAVLKPVAKGYEKVLPEFARTGVTNFFNNLGVVVTMFNDAVQLKGTKVPVDIARFTT
                     NLTFGIFGLIDVASELRIENRNEDFGQSLGYWGVASGPYVVLPLLGPSTVRDGAGLAV
                     DYVTSPFYYWDNQTRVRWEVLAVNIVNTRANLLPAEKVLDESGVERYTFLRDVYLQRR
                     EYLIYDSNPPLSHTGPRSKSLRELEEEDMQDEPAKK"
     gene            3358..4137
                     /locus_tag="VE030_03295"
     CDS             3358..4137
                     /locus_tag="VE030_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZF22474.1"
                     /translation="MDLGIKGKSALVCAASKGLGKGCAFSLAREGVNVTILARGREAL
                     EAAADEIRRATGVKVTVVVTDITTEAGRQAALAACPNPDILINNAGGPPPGDFRNFTH
                     DMWIAAVEANMLTPIELIKATVDGMIGRRFGRIVNITSSAVKAPIDVLGLSNGARSGL
                     TGFVAGIARKTVAHNVTINNLLPGAFDTDRLKSTIVAAAKAQGKPVEEVHAARMKTVP
                     AGRFGNPDEFGDACAYLCSKQAGYITGQNFLIDGGAYPGTF"
     gene            4191..4586
                     /locus_tag="VE030_03300"
     CDS             4191..4586
                     /locus_tag="VE030_03300"
                     /inference="COORDINATES: protein motif:HMM:NF008911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HZF22475.1"
                     /translation="MLWNFGGLVKSHKDLDVWRLAIDLAQDVYEVSKVFPRDEQFGMT
                     SQMRRAAVSIAGNIAEGAARNGQREFTQFLYIALGSASEMETQLEIARRVGLVAEGDF
                     ARLDALLARVAQMLRGLIRSVKSDLDSQS"
     gene            4645..6126
                     /gene="gabD"
                     /locus_tag="VE030_03305"
     CDS             4645..6126
                     /gene="gabD"
                     /locus_tag="VE030_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HZF22476.1"
                     /translation="MNAPRDANFSLQLKDQKLFRQQAYINGEWRDADSKATINVTNPA
                     DGSVIGTIPRMGAAETRRAIEAANAALPAWRARLAKERANILRRWFELMIANTDDLAL
                     IMTTEQGKPLAEARGEIAYAASFIEWFAEEAKRVYGDVIPQHQADKRLVVIKQPVGVC
                     GLITPWNFPAAMITRKAAPALAAGCTVVIKPATQTPYSAFALAELAERAGFPSGVVNI
                     VSGSAKEIGGELTSNTTVRKISFTGSTEVGSVLMRQSAETIKKVSLELGGNAPFLVFD
                     DADIDAAVEGAMISKYRNNGQTCVCANRILVQGGVYDRFAAKLAEKVRALKVGRGTEA
                     GVNIGPLIDENAVAKVEEHIQDAVSKGAKIVVGGKKHVLGGLFFEPTVLTGVNTTMKV
                     TKEETFGPVAPLFRFRTEEEGVAMANDTEFGLAAYFYARDIGRIWRVGEGIESGMVGV
                     NTGLISNEVAPFGGVKQSGIGREGSKYGIEEYLEVKYLCIGGL"
     gene            6302..7969
                     /locus_tag="VE030_03310"
     CDS             6302..7969
                     /locus_tag="VE030_03310"
                     /inference="COORDINATES: protein motif:HMM:NF036723.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZF22477.1"
                     /translation="MPFNQPAPELGNQYSDDRVLRSYLARALPAEMLAAIVPELSELG
                     ELAGGELYRMQIADRLNEPRHTPWDAWGNRIDLVEVSPLWKLAERIAAERGLVATAYE
                     RKHGRFSRIHHFALVHLFTPATDVYSCPLAMSDGAARALVASGNQALIERALPRLTAR
                     DPARFWTSGQWMTESTGGSDVGRSETIARRDGDRWRLYGRKWFTSAVTSQMALTLARP
                     EGNPGGGKGLALFFLETRDANGRLANILVHRLKDKLGTRKVPTAELTLDGTSAWAVAG
                     ETEGVRNIAPMLNVTRTWNAVSAVSYMRRGLALARDYAKKRIVFGAPLADKPLHADTL
                     AGLQAEYEAGFQLTFYAVELLGRDDADCLDAGGKDLLRLLTPIVKLTTGRQAVALLPE
                     IVEGFGGAGYVEDTGIPVLLRDAQVFSIWEGTTNVLALDALRALQAGGALGALQVEVE
                     TLASTVRDTRLVACVKAAQTALAHAQSWISQASGDALEAGARRFAMTLGRALALLLLC
                     RHAQWSLDHEKDQRPLYATRRFAQNGVDMIAAEDVGASAALANDSPA"
     gene            7998..8405
                     /locus_tag="VE030_03315"
     CDS             7998..8405
                     /locus_tag="VE030_03315"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HZF22478.1"
                     /translation="MLCEALEEIMSGVRRFEDLVAWQRARELNREVYTITRTGAFSRD
                     FSLTNQIRSAAASIMSNIAEGFERGGPAQFHQFLVVAKASCGEVRSQLYLALDVGYLD
                     KKEFQQLYGMAEMVGKIIGALRVAVQRRRDHKK"
     gene            complement(8508..9149)
                     /locus_tag="VE030_03320"
     CDS             complement(8508..9149)
                     /locus_tag="VE030_03320"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008674 -
                     2-dehydro-3-deoxy-6-phosphogalactonate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0034194 - D-galactonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HZF22479.1"
                     /translation="MNIEAFGNWLKRWPLVAILRGIKPGEALDIGTMLVETGFTIIEV
                     PLNSPEPFASIMRLAKRLGDRALVGAGTVTDWEQVTKVSDAGGRVIVMPHADERVVEA
                     AKRRNLFVVPGFATATEAFRMINAGADAIKLFPAEANPPKVLKSLRAVLPKDMLILPV
                     GGITPHNMNDYWAAGADGFGLGSALYKPGMTTEQVAQSAAAFRAALHALPKRP"
     gene            complement(9146..10018)
                     /locus_tag="VE030_03325"
     CDS             complement(9146..10018)
                     /locus_tag="VE030_03325"
                     /inference="COORDINATES: protein motif:HMM:NF016894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HZF22480.1"
                     /translation="MIAIDWGTSSLRVYRLDAHGGVLEMRSSPRGILSVENGKFAAVL
                     EAQAEDWIAAGDGPVMMSGMIGSRQGWLEVPYAACPAGFEEIATGMRKVTWDGGTAWI
                     APGLSCHDEAGVPDVMRGEEVQILGVLDRLGPGAHTVCLPGTHSKWVEVTDGRIMRFT
                     THMTGEAFAVFKAHSILGRMMKDGVSDAAAFEDGVRRSADGGGLLHHLFGVRTRGLFG
                     DLTEAIAASYLSGMLIGHEIRSAHGRRGRVHMLCSAQLAEIYARAFRVLGIEANALDP
                     DSVTRGLFRVAAYL"
     gene            complement(10052..10276)
                     /locus_tag="VE030_03330"
     CDS             complement(10052..10276)
                     /locus_tag="VE030_03330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HZF22481.1"
                     /translation="MNAVNVAITQRGGEHEQFLHFALGSAGELDTQLEIAMRVGLAKP
                     AVLEKLQASTTRVGQMLRGLARSLCQKDGA"
     gene            complement(10309..10713)
                     /locus_tag="VE030_03335"
     CDS             complement(10309..10713)
                     /locus_tag="VE030_03335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HZF22482.1"
                     /translation="MRVVVDTNVFVSSFFGGNPRKIIDLWRNGEITLCLSKEIVDECV
                     EVMERLRLPQKLVSEILTLFASGFNLMFAGKTPTLSIVSADPDDNKLFECAVALKAQI
                     IVSGDKAVIGVRDYMGIKVENPQQFLTRTRDA"
     gene            complement(10697..10963)
                     /locus_tag="VE030_03340"
     CDS             complement(10697..10963)
                     /locus_tag="VE030_03340"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HZF22483.1"
                     /translation="MGDQILIRIDPALKEQLVRVAKMEGKTSSEVVRSLIEEYLKQRD
                     IGSYIDSLWERIGKDLKNRKLREKDVTAAIKAVRKARRAHAGSR"
     gene            complement(11074..11919)
                     /locus_tag="VE030_03345"
     CDS             complement(11074..11919)
                     /locus_tag="VE030_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020693836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZF22484.1"
                     /translation="MRERNLSCLSPHGFHRVNYYEWGDPENPKVVICVHGVTRNGRDF
                     DDIARALSPDYRVLCPDVVGRGKSEWLTHKEDYGYPLYCSDTAALIARSGAQQVDWVG
                     TSMGGLIGMFLAAQPNSPIRRLVMNDVGAFIPKASLERLSDYVGKDPRYASFDEFEQY
                     IRAVSAPFGPLTDAQWRHLAQTNARQFEDGRWGPNYDPALGLAFRVTPADVVLWPFWD
                     QVRCPTLVLRGADSDLLLAETAQDMTRRGPKAKLVEFVGIGHAPMLMEPGQIISVREF
                     LLGRG"
     gene            complement(12070..>12468)
                     /locus_tag="VE030_03350"
     CDS             complement(12070..>12468)
                     /locus_tag="VE030_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011301526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZF22485.1"
                     /translation="YLQLKDFKEGRRKDPLMSPMAANLSKKDMLDLAAYFAAQKPTSQ
                     NSRGDASKAARGKQVADAALCTMCHLGGFSGQNEIPRNAGQHYEYLVKQLKDFKVKNR
                     TNDAGNMTAVMRTISDEDIEALAAYIASLN"
BASE COUNT         2358 a         3784 c         3898 g         2428 t
ORIGIN      
        1 tgccgccgcg ctcgccgggt tgaacgtcac cgacgtctcc gggcaaactg tgccgcccga
       61 tttcagaaac aagaagattc acaaatgctg gcgcatttgc agggacgagg gaaagcaagg
      121 atgaaggcgg aaggcggaag gatgaagtaa tttcaaaggc tgttgactct ccttcatcct
      181 tcatccctca tccttcatcc ttggatgctc gtccttccct gtgctacctt cccatcgcca
      241 acgttaaccg accagcatgg ctgacgaacc cgagattccc gcaagtctgc gcgagcaggt
      301 gatcgcgttt tttcatagcg acgccggatc ggatcacgac gccgtcgagc tggcggaccg
      361 cttcgcgacc atcgccgccg ccggcaatct gccggaccgg ctcgatgcgc tcgtgcggct
      421 gatcggatgg acgcacgtgg ccgatgtgga tggcggcacg ggcgtcccgc ccgagcgttc
      481 ccgattgttc aagttcgtgc aggtgatcga atcggtgccg ccagcgcgcc gcagcgtgca
      541 ggacacattc ggcgaaattc tttccgaaac cgagggcgta aatcttttcg gcgacagcgg
      601 aattcccagc gatcgcgcat ttctggccga gttcgtcacg ctggtcatgc ggcgattgct
      661 gccgcagcct agcgaccagc acgatctgtc ccggctggtg gcccgcctgt attcaggcag
      721 ggatgcagtg gagcgattgc gcacgctggc gcctgaggcc ttccatcgcc tcctcgaacc
      781 cctgacgcca ccggagcggc cggaaatatg ggcgccgctg aggacggcgt tttccgacgg
      841 cttccggctg ctggcgatcc ggattgaggc gcagggcttg tccgccaagc tgcgcgcgcg
      901 cagccatccg accccggtgg ccaattcacc gtttcaccgg attgcctgca ccagccatgc
      961 gctcatggat gcatggaaaa ccggagagga catcgccgcg cttttgcggt cctggcgcgg
     1021 gcagagcgcc gaatgtcgcg aagaaatcgc cgaaatcacc cgccgcctgg aaagtgcagg
     1081 ggtgagcgtg gacatcgtct acgggatcga agtgctgaaa cgctgtctcg tgcgcatgga
     1141 agccatgctc gaattcatcg aatcgccacc gggtccggca cgcagcgcgg cgatttaccg
     1201 gctgctggtc gggctcatcg tcgcggcgca cgaggaccgc agcattcgcc acctgatccg
     1261 tgtcgacttg catctgctcc agcgcaagat cgtggaccgt gccggcaaga ccggcgagca
     1321 ctacgtggcg cgcaatctgc gcgaatacca tggtatctgg tttgcggccg ccggcggtgg
     1381 attgctcacg gtcctcaccg ccgcggtcaa gctcaaggtg acgcatgccg ggttgccgct
     1441 gttcgtcgaa ggcatgctct ccggattgaa ctacgcagtc agcttcatgc tgttgcacca
     1501 cttccatctg atgctcgcga ccaagcagcc ggcgatgacg gccgccacgc tggcgaccct
     1561 gctgcacagc cgcgaccgcg ccgagcggct cgaccgcgtg gtcgagttca cgatgcgcat
     1621 ctgcagttcg cagtttgcgg ccgcggttgc caacgtgatt tttgtgttcc tcggggcatt
     1681 tgttttcagt tatatctgga atctggcgct gggccgcgat tacctcggta tcaaggaagc
     1741 ccagcaggtg ttccggacgc tgagtccggt cagcagcggc acggtgtttt acgcggcgct
     1801 caccggcgtg attctgtgga tggcggcgat gatcggcggc tggttcgaca actgggcggt
     1861 gtatcagcgc ctgccgcagg caatcgccga tcaccgcctc gggcagcgtc tcgggcgcaa
     1921 gcgcatggcg cgcgttgccg gcatcgttgc gcgcaacatg gctgggtggg ggaccaacat
     1981 ctcgctcggc ttcctgctcg gcctcacgcc ggtattcggc atgtttctgg gattgccgct
     2041 cgacgtgcgc catgtgacgc tgtcgtcggg aatgctggca tttgcctgcg ccagcctcga
     2101 cggctggttt accgctggct ggtttgtctg ggcgcttgcc ggcgtggcga cgatgttcgt
     2161 gctcaacctc tcggtgagtt tcttcctgtc gctctacacg gctgcgcgcg cctacgaact
     2221 ggaacggcgg gaactggtgg tgctgggcat gcgactgctg cgccggctca tgtccagtcc
     2281 gctggatttt gtgctgccgc gacggtttgt ggagagtgag ttaaaagtgg aaagctaaga
     2341 ggtgaaagtt aaaaggtgaa cgtaaaagaa ggcacatcgt ttcgaccctc gcttccggtc
     2401 gtttaccttt gacctttcac ctgttaccta ttttttcgcc ggctcgtcct gcatgtcttc
     2461 ctcctccagc tcgcgcaggc tcttcgagcg cgggccggtg tgggagagcg gcgggttgct
     2521 gtcgtagatc aggtattcgc gccgctgaag gtagacgtcg cgcaggaacg tgtaacgttc
     2581 gacgccggat tcatccagaa ccttttccgc gggcagcaga ttggcgcgtg tattgacgat
     2641 gttgactgcc agcacttccc agcggacgcg cgtctggtta tcccagtagt agaacggact
     2701 ggtcacataa tctaccgcca gtcccgcgcc gtcgcgcacg gtgctcggtc cgagtaatgg
     2761 aagcacgacg taaggcccgc tggcgacgcc ccagtaaccc aaagactggc cgaaatcctc
     2821 gttgcggttc tcgatcctga gttcggatgc cacgtcgatc aggccgaaaa tgccgaaagt
     2881 cagattggtg gtgaagcggg cgatgtccac gggcaccttc gtgcccttga gctggaccgc
     2941 atcgttgaac atagtgacca cgactccaag attgttgaag aagttggtca ccccggtacg
     3001 cgcgaattcc ggcaggactt tttcgtaccc tttcgcgacc ggcttgagca cggccttgtc
     3061 cagtccctcg ttgaatgcaa agaccttgcg gttgaatgcc tcgtacggat cgcgcggatt
     3121 ggggccgacg gcgcaacctg ccagaagaca tgcacaggcc aggacgagtg gcgaaagggg
     3181 gcgcggattc atgatggctt gcattatggc gggcggcggc gattatatcg cggcacccgg
     3241 cgggccgatt agaacattat tgcggtttgc aaaacccgcc tgcttgcgtc acgatattcg
     3301 ccaccggccg ctctagccgt cattcgaaca agacacggac aaaaaggatc gaattccatg
     3361 gatctcggca tcaagggaaa atctgcgctg gtgtgcgcag cgagcaaggg gctcggcaag
     3421 ggctgtgcat tctcgctggc ccgggaaggt gtcaacgtga ccatcctcgc ccgcgggcgc
     3481 gaggcgctgg aagccgcggc ggatgaaatc cgcagggcaa cgggcgtgaa ggtcaccgtc
     3541 gtggtaaccg acatcacgac ggaagcgggg cgccaggcgg cgctcgcggc gtgtccgaat
     3601 ccggacattc tcatcaacaa tgccggaggc ccgccgccgg gcgacttccg caatttcacg
     3661 catgacatgt ggattgcggc tgtcgaggca aacatgctga cgcccatcga actgatcaag
     3721 gccacggtgg acggcatgat cgggcgccgg ttcggccgca tcgtcaacat cacctccagc
     3781 gccgtcaagg cgcctataga cgtcctcggc ctgtccaacg gcgcgcgctc cggcctgacc
     3841 ggcttcgttg ccggtattgc ccgcaagacc gtggcgcaca acgtcaccat caacaacctg
     3901 ctgccgggtg ccttcgacac cgaccggctg aagagcacca tcgtcgccgc cgcaaaggcc
     3961 caaggcaagc cagtggagga agtgcacgcc gcgcgcatga aaacggttcc ggcgggccgc
     4021 ttcggcaacc ccgacgagtt cggcgacgcc tgcgcctatc tttgcagcaa gcaggccggc
     4081 tacatcaccg gccagaattt cctcatcgat ggtggagcgt atcccggtac tttctgaaag
     4141 aaagtaccgg ggcgtgactg cgtgactatc gtgactttcg tgactgcgtc gtgctgtgga
     4201 acttcggcgg actggtgaaa tctcataagg acttggacgt atggcggctg gcgatagatc
     4261 tggcccagga cgtctatgag gtcagcaagg tttttccacg cgacgagcag ttcggcatga
     4321 cgtcgcagat gcgccgagca gcggtctcga ttgcgggcaa catcgccgag ggtgccgccc
     4381 gaaatggcca gcgcgaattc acgcagtttc tctacattgc cctgggttcg gctagcgaaa
     4441 tggagactca gttggaaatt gccaggcggg taggcttggt ggccgaaggg gattttgcgc
     4501 gacttgatgc gttgttggcg cgagttgcgc agatgctgcg gggattgatc cgaagcgtaa
     4561 aatcagatct ggatagtcaa tcgtagcccc acgacagtca cgatagtcac gtcgtcacgt
     4621 cagtcacgat atccgaggtt tccaatgaac gctccccgcg acgccaattt ttcgcttcaa
     4681 ctcaaagacc agaaactttt ccgccagcaa gcctatatca acggcgaatg gcgggatgcc
     4741 gattcgaagg cgacgatcaa cgttaccaat ccggccgacg gctcggtgat cggcacaatt
     4801 cccaggatgg gtgctgcgga aacgcgccgc gccatcgaag cggcgaacgc cgcgctgccg
     4861 gcgtggcgcg cgagactggc caaggaacgc gccaacattt tgcgcaggtg gttcgaactg
     4921 atgatcgcca acaccgatga tctggcgctg atcatgacca ccgagcaggg caagccgctg
     4981 gccgaagcca ggggcgagat cgcctacgcc gcctctttca tcgaatggtt cgccgaggaa
     5041 gccaagcgcg tgtacggcga cgtgatcccg cagcaccagg ccgacaaacg cctggtggtc
     5101 atcaagcagc cggtcggcgt gtgcggcctg atcacgccgt ggaatttccc ggcggcgatg
     5161 atcacccgca aggcggcacc ggcgctcgcc gcgggctgca cggtggtgat caagccggcc
     5221 acccagacgc cttactccgc gtttgcgctg gccgaactgg ccgagcgtgc cggcttcccc
     5281 agtggcgtcg tcaacatcgt cagcggcagc gcgaaggaga tcggcggcga gctgacttcg
     5341 aacacgacgg tgcgcaagat cagcttcacc ggttccaccg aagtcggttc tgttctgatg
     5401 aggcagagcg cggaaacgat caagaaagtc tcgctcgaac tcggcggcaa cgcgccgttc
     5461 ctggtgttcg acgacgcgga catcgacgcg gcggtcgaag gcgcgatgat ttccaaatac
     5521 cgcaacaatg gtcagacctg cgtttgcgcc aaccgtattc tggtgcaggg cggcgtatac
     5581 gacaggttcg ccgccaaact cgccgagaaa gtgagggcgc tcaaggtcgg ccgcggcacc
     5641 gaagccggcg tcaacatcgg cccgctgatc gacgaaaacg cggtggcgaa agtcgaggag
     5701 cacatccagg atgcggtttc caagggcgcc aagatcgtgg tcggcggcaa gaaacacgtg
     5761 ctcggcggcc tgttcttcga gcccaccgtg ctcaccggag tcaacacgac catgaaggtg
     5821 accaaagagg agactttcgg gccggtggcg ccgctgttcc gcttcaggac cgaggaagaa
     5881 ggcgtggcga tggccaacga caccgagttc gggctggctg cttatttcta cgctcgcgat
     5941 atcggccgca tctggcgcgt cggcgaaggc atcgaatccg gcatggtcgg cgtcaacacc
     6001 gggctcattt ccaatgaggt cgcacccttc ggtggcgtca agcaatccgg catcggccgg
     6061 gagggatcga agtacggcat cgaggaatat ctggaagtga agtatttgtg catcggcggt
     6121 ctttgaccgt cgatgaaggg gcgagggacg agggacgcgg gccgagtaaa ttcattgaga
     6181 ccttttcact gcgccgcccc ccaattggaa actcaaggcg gagaacactt gcaagcagta
     6241 gtgaatgtat tgtgtttttc tactcggccc tcgcccctcg gccctcatcc ctgatttccg
     6301 catgcctttc aaccaacccg ccccagaact cggcaatcaa tactcggacg accgcgtgct
     6361 gcgctcctac ctggcgcgcg cactgcccgc cgagatgctg gcggcgatcg tgccggagtt
     6421 gagcgaactg ggcgagctcg ccggcggcga gttgtaccgg atgcagatcg ccgaccgtct
     6481 caacgaaccg aggcacacgc cctgggacgc ttggggaaac cgcatcgatc tcgtcgaagt
     6541 atcgcccctg tggaaacttg ccgagcgtat tgccgccgaa agggggttgg tggccactgc
     6601 ctacgagcgc aagcacgggc gcttttcgcg catccaccac ttcgcgctgg tgcatctgtt
     6661 cacgccggcc accgacgtct acagctgccc gctggcgatg tccgacggcg cggcgcgggc
     6721 gctggtggca tcgggcaatc aggcgctgat cgaacgtgcg ctgccgcggc tgactgcgcg
     6781 cgatccggcg cgcttctgga ccagcggcca gtggatgacc gagagcacgg gaggatccga
     6841 cgtcggccgc tccgagacga ttgcacgcag ggacggcgat cggtggcgcc tctacggccg
     6901 caaatggttt acctcggcag tgacgtcaca gatggcgctg acgctggcgc gccccgaggg
     6961 caatccaggc ggcggcaaag ggctggcact gtttttcctg gagactcgcg acgcgaacgg
     7021 gcggcttgcc aatatcctgg tgcaccggct caaggacaag ctcggcacgc gcaaggtgcc
     7081 cactgcggaa ctcacgctcg acggcacctc cgcctgggcg gtggcgggcg aaacggaagg
     7141 cgtgcgcaac atcgcgccga tgctcaacgt cacccgtacc tggaacgccg tgagcgcagt
     7201 gtcctacatg cgccgcggac tggcgctggc gcgcgactac gccaaaaaac gcatcgtctt
     7261 cggcgcgccg ctcgcggaca agccgctgca cgctgatacg ctggccggac tgcaggcgga
     7321 gtacgaggcc ggatttcaac tgacgttcta cgcggtcgag ttgctcggcc gcgacgacgc
     7381 cgactgcctc gatgccggcg gcaaagatct gctgcggctg cttacgccga tcgtgaaact
     7441 taccaccggc aggcaggcgg tggcgttgct gccggaaatc gtcgagggtt tcggaggggc
     7501 aggctatgtc gaggacaccg ggattcccgt gctgctgcgc gacgcgcagg tattctcgat
     7561 ctgggaaggg accaccaatg tactggcgtt ggacgcattg cgcgcactgc aagccggggg
     7621 cgcgctcggt gcactgcagg tcgaagtgga aacgctcgcc tcgacggtgc gcgacacgcg
     7681 gttggttgcc tgcgtcaaag cggcccagac ggcgctggcg catgcgcaga gctggatttc
     7741 gcaagcttcc ggcgatgcgc tggaagcggg tgcgcggcgc ttcgccatga cgctgggccg
     7801 cgcgctggcg ttgctgctgc tttgccgcca cgcgcagtgg tcgctcgacc acgaaaagga
     7861 tcagcgcccg ctctatgcca ctaggcgctt cgcgcaaaac ggagtggaca tgatcgcggc
     7921 ggaagatgtc ggtgcgtcgg cggcacttgc gaatgacagc ccggcgtgac gaagcacgcg
     7981 aacgtgccga gtgctgagtg ctgtgtgaag ccctggagga aattatgagc ggtgttcgca
     8041 gattcgagga tcttgttgcc tggcagagag cgcgtgagtt aaatcgagag gtttacacca
     8101 ttacaagaac tggggcgttt tccagagact tctccctcac taatcaaatt cgaagtgccg
     8161 ccgcttcaat tatgtcgaac atagcggaag gctttgagag gggtggtccg gcacagttcc
     8221 atcaattcct tgttgtagcc aaggcgtctt gtggagaggt tcgttctcaa ctctatcttg
     8281 cgctggatgt tggatatcta gacaagaaag agtttcagca actttacggg atggcagaga
     8341 tggttgggaa gatcatcgga gctctacggg ttgcggttca gcggcgccgc gatcacaaga
     8401 aatgagtttg ttcagttttc tcagtactca gtttccagag cttccagcaa ggctttgggc
     8461 tttttctcgc agcactcagc acgcagcaca cagcacctgt ttgcacttca cggccttttc
     8521 ggtagagcgt gaagtgcagc ccgaaaagcc gccgccgatt gcgctacctg ttcggtggtc
     8581 atgccgggtt tgtagagcgc cgagcctagc ccgaatccgt cggcaccggc ggcccagtaa
     8641 tcattcatgt tgtgcggcgt gatgccgccc accggcagta tcagcatatc cttgggcagc
     8701 accgcgcgca gcgacttgag cacttttggc ggattggctt ccgcgggaaa tagcttgatc
     8761 gcatccgcgc cggcgttgat catccgaaac gcttcggtcg ctgtggcgaa gcccggcacc
     8821 acgaagaggt tgcggcgctt ggccgcttcc acgacgcgct cgtccgcatg cggcatgacg
     8881 atgaccctgc cgccggcatc ggagactttt gtcacctgct cccagtcggt caccgtgccg
     8941 gcgccgacca acgccctgtc gccaaggcgt ttggccagac gcatgatgct cgcgaagggc
     9001 tccggagaat tgagcggcac ctcgatgatg gtgaagccgg tctcgaccag catcgtgccg
     9061 atgtccagcg cttcgccggg cttgatgccg cgcaggatcg ccaccaatgg ccagcgcttc
     9121 agccagttgc cgaacgcttc gatgttcata gataagccgc cacgcggaat agtcccctgg
     9181 tcacggaatc cggatccagg gcgttcgctt cgatccccag caccctgaag gcgcgagcgt
     9241 agatctccgc gagctgcgcg ctgcacagca tgtgtacgcg gccgcgtctg ccgtgcgccg
     9301 accggatctc gtggccgata agcataccgg agagatacga ggccgcgatg gcttcagtga
     9361 ggtcgccgaa caggccgcgc gtccggacac cgaacaagtg atggagcagg ccgccgccat
     9421 ccgccgagcg gcgtacgcca tcttcgaacg cggcagcgtc gcttacaccg tccttcatca
     9481 tcctgccgag aatgctgtgt gccttgaaaa cggcgaacgc ctcgccggtc atgtgcgttg
     9541 taaaacgcat gattctaccg tccgtcacct cgacccattt gctgtgcgtg cccggcaggc
     9601 agaccgtatg cgcccccgga ccgagccggt ccagcacgcc gaggatctgt acctcctcac
     9661 cgcgcatcac gtccggaacg cccgcctcgt cgtggcagga gagtcccggc gcgatccagg
     9721 ccgtgccgcc gtcccaggtt actttgcgca tgccggtggc gatttcctcg aatccggcag
     9781 ggcaggcggc gtacggaact tccagccagc cctggcggct gccgatcatt ccgctcatca
     9841 tcaccggtcc gtcgccggcc gcaatccagt cttcagcctg cgcttccagc acggcggcga
     9901 acttgccgtt ctccactgaa agaattcccc gtggcgaaga gcgcatttcc agaacgcctc
     9961 catgggcgtc cagacgatat acgcgaagcg agcttgttcc ccagtcgatg gcaatcacgt
    10021 gaataacatg aatatcgtga atgtcgtaag gtcaggcccc atctttttgg cacagggaac
    10081 gcgccaagcc acggagcatc tgtccaactc ttgttgtaga agcttgtagc ttctccaata
    10141 cggcaggctt tgccaggccg acacgcattg ctatttccaa ctgggtatcg agttcgccgg
    10201 ccgaaccaag ggcgaaatgc agaaactgtt catgctcgcc tcctctttgg gtaatggcga
    10261 cattcacggc attcacgaca ttaacgaccg ctccgaatgg tgacgccgtc acgcgtcacg
    10321 cgttctcgtg aggaactgtt gaggattttc gaccttgatg cccatgtaat cgcgcacccc
    10381 gattacggcc ttgtcgcccg acacgatgat ttgtgccttc agtgcgacag cgcattcgaa
    10441 gagtttgttg tcatcgggat cggcggaaac gatgctcagg gtgggcgtct tgccggcaaa
    10501 catcagattg aaaccgcttg caaaaagagt caggatctcc gagaccagtt tctgcggcag
    10561 gcgcaggcgt tccatgactt ccacgcactc gtccacgatc tccttcgaaa ggcagagcgt
    10621 gatctcgccg tttctccaga gatcgataat ctttctcggg ttccccccga agaaggagga
    10681 aacaaacacg ttggtatcaa cgactacccg catgcgctct gcgagccttg cgaacggcct
    10741 tgatggctgc ggtcacgtcc ttttcgcgga gtttgcggtt cttcagatct tttccgatcc
    10801 gttcccagag cgaatcgata taagacccga tatccctctg cttcaggtac tcctcgatca
    10861 acgaacgcac cacctcgctg gatgtcttgc cttccatctt tgccacgcgc accagttgct
    10921 ccttcagtgc ggggtctatg cgaatcagga tctggtcacc catggcgggt ctccggtctg
    10981 tatatctcgt atatcttaga taagattctg gcgaggttca agcaggcgtg gcagatggcc
    11041 taaaaacagc cgaaggggct gccggcgcga cgatcaacct cgtccgagaa gaaattcccg
    11101 gacagagatg atctggcccg gttccatcag catcggcgcg tggccgatgc cgacgaactc
    11161 gacgagcttc gccttcggac cgcgccgggt catatcctgc gccgtctccg cgagcaggag
    11221 gtccgaatcg gcgccgcgca gaaccagcgt cgggcagcgc acctggtccc agaacggcca
    11281 cagcaccacg tcggccgggg tgacgcgaaa ggccagcccc aacgctggat cgtagttcgg
    11341 gccccagcgg ccgtcttcga actgtcgcgc attggtctgc gccaggtgcc gccactgcgc
    11401 atcggtgagc ggcccgaaag gcgccgacac ggcgcggatg tactgttcga attcgtcgaa
    11461 ggacgcgtag cgcggatctt tgccaacata gtcggaaagg cgctcgagcg acgccttggg
    11521 aatgaaggcc ccgacgtcgt tcatcaccag gcggcggatc ggcgaattgg gttgcgcggc
    11581 gaggaacatc ccgatcagcc cacccatcga ggtgccgacc cagtccacct gctgcgcgcc
    11641 gctgcgggcg atcagtgcgg ccgtgtcgga gcaataaagc ggatagccgt agtcttcctt
    11701 gtgcgtcagc cactcgcttt tgccgcggcc aactacgtcg gggcagagca cccggtaatc
    11761 cggcgacagc gcacgcgcga tgtcatcgaa gtcgcgcccg ttgcgagtta ccccgtgtac
    11821 gcaaatgacg accttcggat tttccgggtc gccccattcg tagtaattga cacggtgaaa
    11881 gccgtggggg ctgaggcaag agagattgcg ttcgcgcatg gaagtagctg gtggctggca
    11941 gggccgagct tcgaacgaaa tcaaggatga cggcggaagg cggaaggatg aagtaagttc
    12001 aagggcttct gactctcctc catccttcat ccctcatcct tcatccttgg atgctcctcc
    12061 cccggccctt cagttcaggg atgcaatata cgccgccagc gcttcgatat cttcatccga
    12121 aatcgtcctc atgaccgcgg tcatgttgcc cgcgtcgttg gtccggttct ttaccttaaa
    12181 gtctttcagt tgcttgacta ggtactcgta gtgctggccg gcgttgcgcg ggatttcgtt
    12241 ctgacccgag aaaccgccga ggtggcacat ggtgcacagc gcggcgtcgg caacctgttt
    12301 gccgcgggcc gccttggaag catcgcccct ggagttctgg ctggttggtt tctgcgcggc
    12361 gaagtaggcg gcgaggtcga gcatgtcctt tttcgacagg ttcgcggcca tcggcgacat
    12421 cagcgggtcc ttgcggcgac cctctttgaa gtccttgagt tgcaggta
//
LOCUS       DASQOP010000087        22078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2649757, whole genome shotgun sequence.
ACCESSION   DASQOP010000087 DASQOP010000000
VERSION     DASQOP010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 22078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22078
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2649757"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VE030_03355"
     CDS             complement(<1..530)
                     /locus_tag="VE030_03355"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HZF22486.1"
                     /translation="MRASLSTVLVGVVVVLIVASMSLFTVDQRQNAMVFRLGEVISVK
                     TEPGLYVKMPVIDNVRFFDTRILTLDSEEPERFLTSENKNVLVDSFVKWRVTDVRQYY
                     VSVGGDELRANDRLKKTVNSSLRDEFGKRTVHDVVSGERDKIMEVMREKVDDDASKIG
                     VKVLDVRLKRVDLTQEV"
     gene            complement(527..1705)
                     /gene="hflK"
                     /locus_tag="VE030_03360"
     CDS             complement(527..1705)
                     /gene="hflK"
                     /locus_tag="VE030_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HZF22487.1"
                     /translation="MALNDPNWGKRGNEGPPDLDELWRRLNQKFGSLFGGKGGGNVPQ
                     GPSARQMGGGFGLLIIMVLLVWLASGFYIVDEGQRGIVLRFGRFLETNQPGPRWHLPW
                     PVESVEIVNVSEVRTVEVGYRNSVRTKVPNESLMLTDDENIIDLQFAVQYIRANPEEY
                     LFNNRDADHAVMQVAESAIREVVGKNKMDFVLNEGRAQVAAKAQELMQVILDRYKTGI
                     SISQVTMQNAQPPEQVQAAFDDVNKANQDRERQKNEGQAYANDVVPKARGTAARLIQE
                     AEGYRQRVVANAEGEASRFRQIVAEYSKAPGVTRDRLYLDMMQQVLTNSSKIVVDQKA
                     GSNLLYLPLDKLMQLGGSGSSGDVGKPAVPEVPMPVPGPAEATPRSREAFRNRDREAR
                     "
     gene            complement(1752..2870)
                     /gene="hflX"
                     /locus_tag="VE030_03365"
     CDS             complement(1752..2870)
                     /gene="hflX"
                     /locus_tag="VE030_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HZF22488.1"
                     /translation="MLERPRGGDRAVLVNLDFGDVDFAESLEEIRRLAESADVKPVTV
                     VKGRRQRPDAATFAGSGKVEEIAALVQSTGASLVLFNHDLSPAQQRNLEKRCNCRVID
                     RTSLILDIFAQRARSHEGKLQVELAQLEHLSTRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRQLLRKRVKVLKDKLATVHRQRQTQRRARLRGPAMSVSLVGYTNAGKSTLFNQLT
                     DSDAYAADQLFATLDTTSRRINGTNGAAVVVSDTVGFIRDLPPALVAAFSATLEETAR
                     ADLLLHVVDAASPARDDQIAEVNKVLAAIGAVAVPQVMVFNKIDAAGLEPGVEHDQYG
                     KIAAVRVSARSGSGVGFLRSLLGDLQEAHRSARGLAAA"
     gene            complement(2976..3215)
                     /gene="hfq"
                     /locus_tag="VE030_03370"
     CDS             complement(2976..3215)
                     /gene="hfq"
                     /locus_tag="VE030_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HZF22489.1"
                     /translation="MSAKGQLLQDPFLNALRKEHVPVSIYLVNGIKLQGQIESFDQYV
                     VLLKNTVTQMVYKHAISTVVPARPVNLSLEHGSED"
     gene            complement(3320..4651)
                     /gene="der"
                     /locus_tag="VE030_03375"
     CDS             complement(3320..4651)
                     /gene="der"
                     /locus_tag="VE030_03375"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HZF22490.1"
                     /translation="MTPTIVLVGRPNVGKSTLFNRMTRSRQALVADIPGLTRDRHYGH
                     GNRDGKSFLVVDTGGFEPIARDGLLKEMARQTMQAVDEADAVLLLVDARQGLTPQDRR
                     IVTELRKAGRRLWLVVNKAEGLAAETAKADFHELGLGEPLAISAAHGEGVRDLVDLVL
                     EQFPAQQQAEDLSRHPKIAVVGRPNVGKSTLVNTLLGEDRVITFDQPGTTRDSIYIDF
                     ERNGRQYTLIDTAGVRRRGKVEDVVEKFSVVKTMQAIEDANVVVLVLDVMQEVSDQDA
                     HLAGFILEAGRALVVAVNKWDAADQESREKAKRDYARKLNFLDFAPLHFISARAGTGV
                     VSMLKSVDTAYASAMAKLSTPKLTRALIAAVQKQSPPRSGVSRPKLRYAHQGGMNPPL
                     IMIHGNGLDHVPDSYRRYLERTFISAFQLSGTPVRVFFKQGRNPYADRKKP"
     gene            complement(4820..5953)
                     /gene="bamB"
                     /locus_tag="VE030_03380"
     CDS             complement(4820..5953)
                     /gene="bamB"
                     /locus_tag="VE030_03380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HZF22491.1"
                     /translation="MRVESLLLRLAIATLIAVLLGGCGSKGPKPAPLPEFKPAAKLHV
                     EWRTNVGSAGYYLFVPAFYRDGVYAAGSGGKIVRLNAATGKTVWRVDTKAPLSGGVGV
                     GENMVMVGTAKGAVLAYDLDGKPLWTSTVSSEVLSAPQAADGFVVVRSGDSRIFGLNA
                     RDGTRRWEYQTITPPLTLRANPGVIIVEGFVIAGMPAGKLVVLNLANGGVVWETVVAA
                     PKGDNELERITDIAGAPLVEQQKVCAVTFRGRAACYETERGGQLWARAASSVGGLAAD
                     ERSVYLSEDTGAVVALDKNTGASAWKQDRLSQRGLSTPLSFGSHVVVGDFEGQVHFLK
                     IEDGSFAARVGTDGSVIAAAPLRVDDKVLVQTRKGGVYAITVK"
     gene            complement(5950..6585)
                     /locus_tag="VE030_03385"
     CDS             complement(5950..6585)
                     /locus_tag="VE030_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZF22492.1"
                     /translation="MALDLEEQEQLDALKQFWKQYGRLIITLAFLGLAAFAGVRGWNY
                     YQRGQSEQASALFGKLEEALRKNDVAQIGSLGAEIIDKYGSTAYGPMAALLLARRNYD
                     SGDAAAAATRLQWVIDKARDDETVTLARLRLAGIRLDEKKYDEALALLDAKHAPAFAP
                     LYADLRGDVLAAQGKAAEARAAYKVALEKSPPNSNYRSVVQIKLDALGPAK"
     gene            complement(6601..7878)
                     /gene="hisS"
                     /locus_tag="VE030_03390"
     CDS             complement(6601..7878)
                     /gene="hisS"
                     /locus_tag="VE030_03390"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HZF22493.1"
                     /translation="MTQSIQAVRGMNDILPDQAPLWEFFEDTVRDWLSQYGYRNIRMP
                     ILEKTELFVRAIGEVTDIVEKEMYTFVDQLNGESLTLRPEGTASCVRAAIQHNLLYSG
                     PQRLFYVGPMFRHERPQKGRYRQFHQVGVEALGFAGPDVDAEHMVMSAGLWRRLGLDG
                     IRLEINSLGNAASRGRYRARLIRYLERHAAELDEDARRRLSTNPLRVLDSKNPAIQQV
                     IAQAPQLADDLDEESLKHFQELQDLLRNAGINFAINTRLVRGLDYYNDVVYEWATTRL
                     GAQGTVCAGGRYDGLIAQIGGKPAPACGFAMGMERLLALLIDADTAAPARIVDVYLVH
                     SGEAAGKFAWTVAETLRGKGLSVVFHCGGGSFKSQMRRADASGARFAVIVGDDEAAKQ
                     QIALKPLREPGEQAQVSADEAASIIRGPNLSTT"
     gene            complement(7916..9202)
                     /gene="ispG"
                     /locus_tag="VE030_03395"
                     /gene_synonym="gcpE"
     CDS             complement(7916..9202)
                     /gene="ispG"
                     /locus_tag="VE030_03395"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HZF22494.1"
                     /translation="MSDCAIRRRSTRQVSVAGVAIGGGAPVVVQSMTNTDTADVEATV
                     RQVAELAEAGSELVRITVNSAEAAAAVAEIRNRLERLGCPVPLVGDFHFNGHKLLTQH
                     PDCAQALDKYRINPGNVGRGSRRDEQFATMIECACRYGKPVRIGVNWGSLDPDLLGRM
                     MDDNARLPEPKDASAVMREALIASALESAAKAEAIGLPGDRIVLSCKVSGVQDLITVY
                     RDLAARSEYPLHLGLTEAGMGSKGIVASAAALAVLLQEGIGDTIRVSLTPDPGGDRAR
                     EVVVAQEILQTMGLRSFTPMVTACPGCGRTSSTFFQELADKIQGYLRQQMPVWRAQYP
                     GVEEMRVAVMGCVVNGPGESKHANIGISLPGSGETPVAPVFVDGVKTVTLKGERIAED
                     FQRLVDDYVADHYGRAGKCAAEQAPHPGNESARATG"
     gene            complement(9214..10011)
                     /locus_tag="VE030_03400"
     CDS             complement(9214..10011)
                     /locus_tag="VE030_03400"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZF22495.1"
                     /translation="MSATATNGQSSVPGGAGAALLMERRRQGLSLGDISRQLKLSVRQ
                     VEALERDDYSGFTGPVFVHGFIRNYAKLLSLDPDPLIRAADQVLHPPLPASEPVAAAA
                     SPLVVRRRRIGMVPAMSAVAAVAVVVAAVAVLVGNRGGSKPASEESAAVEQAALPKQP
                     APAAIPIAAQAAVKPAAGNAVAAEPGERATLRMVFDQESWVEIKDRNGNTIFGQLSPA
                     GSRRSVSGEPPFDVVVGNAVGVRLTYKDEAVDLSAHTQVDVARLTLE"
     gene            complement(10008..10775)
                     /gene="pilW"
                     /locus_tag="VE030_03405"
     CDS             complement(10008..10775)
                     /gene="pilW"
                     /locus_tag="VE030_03405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HZF22496.1"
                     /translation="MTRLVTIVVLAVLAGCATQGQKSAADEQTRADIQNRARIHTELG
                     ALYAGAGQMKTAVDEFKAALAADSGYGPAHNQLGMVYVALGEDRLAQESFERALRIDP
                     NDSAANNNYGMFLCRRKREKEAFKYFMAALKNPLYTTPENAYANAGVCARMQGDDVKA
                     EDWLRKALALQPNQPQALYQLAEMEFKRGNRQGARNLLDRHLQIVSNPSPDALWLGAR
                     VADSLGDRNAVAGYGTQLNNRYPDAVQTKAFNEGRFQ"
     gene            complement(10772..11857)
                     /gene="rlmN"
                     /locus_tag="VE030_03410"
     CDS             complement(10772..11857)
                     /gene="rlmN"
                     /locus_tag="VE030_03410"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HZF22497.1"
                     /translation="MPVNLLDFDSERLAGLFAEIGEKPFRARQVMRWIHQLGEAEFGR
                     MSDLAKDLRAKLDGCAVVEPPSVMAESTATDGTRKWLLSVGTGNGIETVFIPETDRGT
                     LCVSSQVGCALECAFCSTGRQGFNRNLSVAEIIGQLWWANKALGHDPRGERVISNVVM
                     MGMGEPLANFDNVVSAMRIMLDDNAYGLSRRRVTLSTSGIVPAIDRLAAELPVALAVS
                     LHAPDDQLRDRLVPINKKYPLKELIAACKRYIKVAPRDFVTFEYVMLDGVNDGVEHAK
                     QLIALVKGVHCKFNLIPFNAFPDSGFQRSAPERIAAFRKVLMHAGLVATIRKTRGADI
                     DGACGQLAGQVQDRTKRRSRRALEAST"
     gene            complement(11857..12282)
                     /gene="ndk"
                     /locus_tag="VE030_03415"
     CDS             complement(11857..12282)
                     /gene="ndk"
                     /locus_tag="VE030_03415"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HZF22498.1"
                     /translation="MSVERTLSIIKPDAVAKNVIGQIYSRFENKGLKVVAARMLHLSR
                     AEAEGFYAVHRQRPFFSDLVNFMISGPVMVQVLEGESAIQKNRDLMGATDPKKAAKGT
                     IRADFADSIDANAVHGSDAPGTAAVEIAYFFPTLNIYSR"
     gene            12477..13052
                     /locus_tag="VE030_03420"
     CDS             12477..13052
                     /locus_tag="VE030_03420"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HZF22499.1"
                     /translation="MTNNPNNAPAGAPTPERLKQRASRRLLAALLLIAVAIGGLALTE
                     RWRQPPAITPPPHEPSQALIAPPSPEPLAEPLQSDHPAGPPPPPQVVNNEKRVAPAPA
                     KPAPALPATGAKIDAKPAPATDKTYVVQVGVFMSPTNAQALQQQLVKAGIPAHTETRV
                     QLGPFQDRREAETALAKVKKLGVDAVLVAPH"
     gene            complement(13059..14348)
                     /locus_tag="VE030_03425"
     CDS             complement(13059..14348)
                     /locus_tag="VE030_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HZF22500.1"
                     /translation="MNVALILFILLLLLLTGTPISIALGMTVLTFLALFSSFALGTVD
                     IVSQRLFTGLESFAIMAVPFFVLSGTLLTEGGIAARIIRFALALVGWMRGGLAMAAIL
                     ACAFFATISGSSPATVVAIGSIMLPAMVKHGYPKRFAVGVIATSGSLGILIPPSIVMI
                     IYAVSTSESAGKLFMAGVVPGILLAGVLMLVTYVTARAKNFPTMPRASAREVWVSFKE
                     AFWGLMLVVIVMGGIYGGIFTPTEAAAVAAVYSFIAAVCIYRGLKVRDMPKVLLSAAN
                     TSAMLLYIITNAILFSFLLTSEQIPQVLSQWVVDQQLSPWMFLLAVNVALLVAGQFME
                     PSSIILILAPLLLPIAKSLGIDPIHLGVIMTVNMEIGMIHPPVGLNLFVASHIARMGM
                     TEVSIACLPWVFVLLAYLALVTYVPVISLWLPGLLYS"
     gene            complement(14345..14893)
                     /locus_tag="VE030_03430"
     CDS             complement(14345..14893)
                     /locus_tag="VE030_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HZF22501.1"
                     /translation="MGRIFNRVLDRIEEWLVASFMAAATLIIFAAVLHRQSSAIPWLW
                     DYTGALNFSWAQELCIYLFIWMAKFGAAYGVRTGIHIGVDVAVNAAGPRLKRVLVVLA
                     LSLGAFFTAVIAIMGARWVWFIHGTGQVSPDLEWPMWVVYLCIPLGSALMCYRFLQVM
                     ARFVKSGVLPAQAHGAAAEEGA"
     gene            complement(14899..15909)
                     /locus_tag="VE030_03435"
     CDS             complement(14899..15909)
                     /locus_tag="VE030_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HZF22502.1"
                     /translation="MIRLVFAALLFATTISAFAGDPIVIKFSHVVATDTPKGEAAAKF
                     KQLAEKYSSGRVKVELYPNSQLYKDREEMEALQLGAVQMLAPSVSKFGPLGVREFEVF
                     DLPYLFPDAETVDRVTDGEIGQALFAKLESRGIRGLAYWDNGFKQFSANRPLREPGDF
                     RGLKMRIQSSKVLEAMMRQLGALPQVMAFSEVYSALQQRVVDGTENPVSNLYTQKMHE
                     VQSHLTISDHGCLVYAVIVNRKFWDGLPADVRAQLEKAMRETTVYERDVAKKDNEDAL
                     AAVRASKKTEVYVLGDAQRKRWREALLPVHAKFEQTVGRDNLQAIYRVAAAVEAEKAK
                     KK"
     gene            complement(15957..17474)
                     /gene="tilS"
                     /locus_tag="VE030_03440"
     CDS             complement(15957..17474)
                     /gene="tilS"
                     /locus_tag="VE030_03440"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HZF22503.1"
                     /translation="MTERLLETVAAVLNTHAVRGRRVAVGLSGGVDSVVLLDVLGRLR
                     KDLRLALSAVHVNHQISAHAQEWEAFAKTLCQARGVALTVRRVRVPRDGSGVEAAARK
                     LRYEAYGGLEADFVALAHHLDDQVETFLLQLLRGAGAKGLAAMPVLRKYEDGTSKDEG
                     GREKGKDEGGRGKGRDEGGRMKAEGREIASQHDTSSLIPHPSSLDSHPSSFILPPSSL
                     VSHPCILRPLLEVTRGEIEAYAKTRKLEWIEDDSNADSRYDRNFLRNELLPRLEARFP
                     AYRETLSRASRNLADYSELAEELAAFDARAFEDGVIPVESLRGLSDARALNVLRRLFA
                     SRALPMPNRTRLEESLRQCRAAGADSEVHVSFGSHGLRCFRGQVAIVEESGEMPVDWN
                     TQWDGRRELLLPAGLGVLRARAALGEGIAARHFARQGATVRGRTGGERMRPAEDRPRR
                     TLKNLLRETAVPPWERERMPLLFIGDALAWVPGIGVAAEFRAASGEQGIVPEWKR"
     gene            complement(17563..18531)
                     /locus_tag="VE030_03445"
     CDS             complement(17563..18531)
                     /locus_tag="VE030_03445"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017331761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HZF22504.1"
                     /translation="MKTTFLEFEQPIAELESKIEELRYVQDDSAVDISEEIARLQKKS
                     SALTREIYSKLSAWQISQVARHPQRPYSLDYFQNNFTDFEELHGDRAFAEDPSIIGGL
                     ARFGSQTVMVIGHQKGRDTKDKLYRNFGMPKPEGYRKALRLMRLAEKFGIPVMTFVDT
                     PGAYPGLGAEERGQSEAIGRNLYVMAELRVPVVVTIIGEGGSGGALAIAVGDVTLMLQ
                     YSIYSVISPEGCASILWKSADKAPDAAETLGITANRLKTLGLVDSVVTEPLGGAHRDP
                     ATMMQTLRKALQEALAEAQRKSIDELVEARFGRLMAYGKFKEVAVR"
     gene            complement(18621..18899)
                     /locus_tag="VE030_03450"
     CDS             complement(18621..18899)
                     /locus_tag="VE030_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZF22505.1"
                     /translation="MRTILALMMVLAAAPALAQESQLRLRDGPGRQLVEASCVMCHSL
                     DYIPMNSPFLDRKGWEASVNKMIKVMGAPIAEADAQKIVDYLAGQYGK"
     gene            complement(18909..20138)
                     /locus_tag="VE030_03455"
     CDS             complement(18909..20138)
                     /locus_tag="VE030_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZF22506.1"
                     /translation="MQTRRDFLKVSAGGLLLAGGIRGADSAVAADSLGPAALPSGALQ
                     SAQLAALPGKLPLIKKSFRPPNFETPVRFFNEAFTPNNAFFVRYHLADIPQVSQQRWK
                     LAVSGDALDKPFEITYEELSRDFEQVEVAAVCMCSGNRRGLSQPHVPGVQWGHGAIGN
                     ARWRGVRLRDLLAKAGVKKDALEIVFDGTDRGVLDKTPDFVKSIPAWKAMDENTLVAL
                     EMNGEKLPHWNGFPVRIVVPGWTATYWMKHLTSITATAQPYQGFWMNPAYRIPKGKFP
                     LVDRFVSQETEVNTPITEMVVNSLVTNLEDGQKFAFGQEITVAGVAWDGGFGIATVEV
                     SEDGGRTWRAAQLATDLGRFSWRQWSHRFVPGRAGSHMLMARAANRAGATQTFELIFN
                     PAGYHNNVVQRINIEIT"
     gene            complement(20171..21859)
                     /gene="ggt"
                     /locus_tag="VE030_03460"
     CDS             complement(20171..21859)
                     /gene="ggt"
                     /locus_tag="VE030_03460"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HZF22507.1"
                     /translation="MKRPWSGAVLRFAFVVALLAGLAQGPAAAQECGIASAHTLATRA
                     GCDILKSGGNAFDAAVAVAAALAVVEPYASGIGGGGFFLLHRGADAFDVFVDARETAP
                     AKATREFYLDDNGQPRQKASLEGPTAAGIPGMPAALAWVAGHYGKLTLARALQPAIRL
                     AEQGFPADARYVWATGYREAMLKADVNAGRIFLDNGNAVGPGFVVKQPQLARTLRMLA
                     AQGRQGFYRGDFARRLVEAVRAGGGLWELSDLESYRVVERPPAKFSYRGATITSAPLP
                     SSGGLVLAQSLLILERVALATLSETDRVHYTVEAMRRGYHDRARYMGDPDFVAVPVEK
                     LASREYAKQRAASIDPDRATPSAALAEIADAAAEGKDTTHLSVIDAEGNRVAATLSVN
                     APFGSGFVAGDTGVLLNNHMNDFSLAPSAANIYRLVGNEANAIEAGKRPLSSMSPTFV
                     EDERGVLVLGTPGGARIISMVLEGILAYVDQPLFDLQRMVGAPRYHHQYLPDRIEIEP
                     GAFSQQWIAALRAKGHALEEGRRKWGNMQAVYFDRKTGRVTSANDPRGQAGVLF"
     gene            complement(21893..>22078)
                     /locus_tag="VE030_03465"
     CDS             complement(21893..>22078)
                     /locus_tag="VE030_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22508.1"
                     /translation="MDTDAHIREVSSMMRREFPGAFTANFQGDNGLQPMRGDFLRKFR
                     GIAEAYPFRPFLEEVTT"
BASE COUNT         3963 a         7380 c         6650 g         4085 t
ORIGIN      
        1 acttcctgcg tgagatcgac ccgcttgagg cgcacgtcca gcactttgac gccgatcttg
       61 ctggcatcgt cgtcaacttt ctcgcgcatg acttccatga tcttgtcgcg ctcgcccgac
      121 accacgtcgt gcacggtgcg cttgccgaac tcgtcgcgca gactcgagtt cacggtcttc
      181 ttcaggcggt cgttggcgcg cagctcgtcg ccgccgacgc tcacgtagta ttgacgcacg
      241 tcggtgacgc gccatttcac gaacgaatcg accagcacgt tcttgttttc ggaagtcagg
      301 aaccgctcag gctcctctga atccagcgtg agtatccggg tgtcgaagaa acgcacgttg
      361 tcgatcaccg gcatcttgac gtacaggccc ggttcggtct tgacgctgat cacctcgccg
      421 aggcggaata ccatcgcgtt ctggcgttga tcaaccgtga acaggctcat gctggcgacg
      481 atcagcacca ccacgacacc gaccagcacc gtgctcaggc tggctctcat cgcgcctccc
      541 tgtcacggtt gcggaacgcc tcgcgcgagc gcggcgtggc ttccgccggg ccgggaaccg
      601 gcatcggcac ttccggaacc gcgggcttgc cgacgtcgcc ggaacttccg gaaccgccca
      661 gttgcatcag cttgtcgaga ggcaggtaca gcaaattgct gccggccttc tgatcgacca
      721 cgatcttgct cgagttggta agcacctgtt gcatcatgtc cagatagagg cggtcgcggg
      781 tcacccccgg cgccttactg tactcggcca cgatctggcg gaaacgcgac gcctcgccct
      841 cggcgtttgc gaccacgcgc tggcgataac cttccgcttc ctggatcagt ctcgccgcgg
      901 taccgcgcgc cttcggcacg acgtcattgg cgtaggcctg gccctcgttt ttctggcgct
      961 cgcggtcctg attggccttg ttgacatcgt cgaatgccgc ttgcacctgc tcgggcggtt
     1021 gcgcgttctg catcgtcacc tggcttatgg agatgccggt cttgtaccgg tcgagaatga
     1081 cctgcatcag ttcctgcgcc ttggccgcca cctgggcacg gccctcgttg agcacgaaat
     1141 ccatcttgtt cttgccgacg acttcgcgaa tcgccgattc ggcaacctgc atcacggcgt
     1201 ggtcggcatc ccggttgttg aacagatact cttccggatt ggcgcggatg tattgcacgg
     1261 cgaattgcag atcgatgatg ttctcgtcgt cggtcagcat cagcgactcg ttgggaacct
     1321 tggtacgcac gctgttgcgg taccccactt ccacggtgcg gacttcggat acgttgacga
     1381 tctcgaccga ttcgaccggc cagggcagat gccagcgcgg gcccggctga ttggtctcca
     1441 gaaaccggcc gaagcgcagt actatgccgc gctggccttc atccacgata tagaagccgc
     1501 tggcgagcca caccagcagc accatgatga tgagcagtcc gaaaccgccg cccatctgac
     1561 gggcgctcgg tccctgaggc acgttgccac cgcccttgcc gccgaaaagg gagccgaatt
     1621 tctgattcag gcgacgccag agttcgtcga ggtcgggcgg gccttcgttg ccgcgcttac
     1681 cccagttggg atcattcaat gccattgatg cttgctttct tcggacaggg aggcgaaacg
     1741 gggaaaactc tctatgctgc ggcgagtcct ctggcgctgc gatgcgcctc ttgcagatcg
     1801 cccagcaggg aacgcagaaa accgacgccg gaaccgctgc gagcgctgac gcgaaccgcc
     1861 gcgattttac catactgatc gtgctcgacc ccgggttcca gtccggccgc atcgatcttg
     1921 ttgaagacca tcacctgcgg gaccgcgacc gcgccgatcg ctgcgagcac tttgttcact
     1981 tccgcgatct ggtcgtcgcg cgccgggctg gcggcgtcta ccacgtgcag aagcagatcg
     2041 gcgcgcgccg tctcttccag agtggcgctg aaagctgcca ccagagcggg cggcagatca
     2101 cggatgaacc ccacggtatc ggacactacc accgcggcgc cgttcgtgcc gttgatgcgc
     2161 cgcgacgtcg tgtccagcgt ggcgaacaac tggtcggcgg cataggcgtc gctgtccgtg
     2221 agctggttga acagcgtcga tttgccggcg ttggtataac cgaccaggga caccgacatc
     2281 gccgggccac gcagccgtgc ccggcgttgc gtctgccgct gtctgtgcac cgtggcgagc
     2341 ttgtccttga ggaccttcac gcgcttgcgc aacaattggc ggtcggtttc cagctgcgtt
     2401 tcaccggggc cgcgcaggcc gatgccgccc ttctgccgct cgaggtgggt ccagccgcgc
     2461 accagccgcg tggacagatg ttccagttgc gccagttcca cctgcagctt gccttcgtgc
     2521 gaacgggccc gctgcgcaaa gatgtccaga atcaggctgg tgcggtcgat cacgcggcag
     2581 ttgcagcgct tttccagatt gcgctgctgt gccggcgaca gatcgtgatt gaacaacacc
     2641 agcgaggcgc cggtcgactg taccagggcg gcaatttcct cgaccttgcc ggagccggcg
     2701 aacgttgcgg cgtcggggcg ctggcgcctg cccttcacca cggtcaccgg tttcacgtct
     2761 gcgctctcgg ccagccggcg gatttcctcg agcgactcgg cgaaatcgac atcaccgaaa
     2821 tccaggttca cgaggactgc gcggtcgccg ccgcgagggc gttcaagcat cgggaatttt
     2881 caagtaaggc acggcacgcc cgatcccgcc gcgccggctg attgtcgcgg cgacggagca
     2941 tgagagaaag cgacaacggc agatgcgggg accactcagt cttcggaccc gtgctccaaa
     3001 gacaagttca ccggacgcgc aggcacgacg gtggatatcg catgcttgta caccatttgc
     3061 gtcacggtat tcttcagcaa caccacgtat tgatcgaacg actcgatctg accctgcaat
     3121 ttgatgccat ttaccaggta gatcgaaacc ggcacatgct ccttgcgcaa cgcattcaga
     3181 aacgggtctt gtaacaattg ccctttcgcg ctcatggcag ctccccattg tgtttttatg
     3241 acccacatta actctactgg attttgacgc gcaataccat cccgcgaaac cccgcgtggg
     3301 ggacctgatc agagctcccc tagggcttct tgcgatccgc ataagggttt ctgccctgct
     3361 tgaagaatac ccggaccgga gtcccggaca actggaaagc ggagatgaaa gtacgctcga
     3421 ggtagcggcg ataactgtcc ggcacgtggt ccaggccgtt tccatggatc atgatcagcg
     3481 gcgggttcat gccgccctgg tgcgcataac gcagcttggg gcgggacacg cccgagcgcg
     3541 gtggcgactg cttctgcacc gcggcgatca gcgcgcgggt cagcttcggt gtcgaaagtt
     3601 tcgccatggc ggaggcataa gcggtatcca ccgacttgag catcgacacc acgccggtgc
     3661 cggcacgcgc ggatatgaaa tgaagcggcg cgaagtcgag gaaattgagc ttgcgggcgt
     3721 agtcgcgttt ggctttctcg cgcgactcct ggtccgcggc gtcccatttg ttgacggcga
     3781 ctaccagtgc cctgccggcc tcgaggatga agccggcgag gtgggcatcc tgatcggaga
     3841 cttcctgcat cacgtcgagc accaacacca ccacgttggc gtcctcgatc gcctgcatgg
     3901 tcttcaccac cgagaacttc tccaccacat cctcgacttt tccgcgccgg cgcaccccgg
     3961 cggtgtcgat cagcgtgtat tgccggccgt tgcgctcgaa gtcgatgtaa atgctgtcgc
     4021 gggtggtgcc cggctggtcg aaggtgatca cccggtcttc gcccagcagc gtgttcacca
     4081 gcgtggactt gccgacattg ggccggccga ccaccgcgat cttgggatgc ctggagaggt
     4141 cctcggcctg ctgctgcgcc ggaaattgtt ccagcaccag gtcgacgaga tcgcgcacgc
     4201 cttcgccgtg cgccgccgag atggccaatg gctcgccgag tccgagttcg tgaaaatccg
     4261 cttttgccgt ctccgctgca agcccttcgg ctttgtttac caccagccac agcctgcggc
     4321 cggccttgcg cagctcggtg acaatgcggc gatcctgcgg cgtcagcccc tgccgcgcat
     4381 ccaccagcag caacaccgcg tccgcctcat ccaccgcctg catggtctgg cgggccatct
     4441 ccttgagcag gccgtccctg gctatgggtt cgaatccgcc ggtgtcgacc accaggaaag
     4501 atttgccgtc gcggttgccg tgaccgtaat ggcggtcgcg cgtgagcccg gggatgtccg
     4561 caaccagcgc ttggcgcgag cgcgtcatgc gattgaaaag cgtggacttg ccgacgttgg
     4621 gccggccgac cagcacgatc gtgggcgtca tgacaaggtt gaaggcggaa ggatgaaaga
     4681 tgaaggggaa agcattaatg tactggaagc acgtcgggaa gccggcccaa ggaaaaaggt
     4741 gtgccgcttg gcacaccgca cttcatcctt ccgccttgct cttcatcctt catccttccg
     4801 ccttcatact tcatccttgc tatttcacgg tgatcgcata cacgccgccc ttgcgggttt
     4861 gcaccagcac cttgtcatcg acgcgtaggg gggccgccgc aatgacgctc ccatcggtgc
     4921 cgacgcgcgc agcgaacgat ccgtcctcga tcttgaggaa gtgaacctgg ccctcaaagt
     4981 cgccgacgac tacatggctg ccgaaggaca gcggagtact caagccgcgc tgggacagtc
     5041 tgtcctgctt ccacgcgctg gccccggtat ttttgtcgag cgccaccact gcgccggtgt
     5101 cttccgacag atacacgctg cgctcgtcgg ccgcgaggcc gccaacgctg gatgcggcgc
     5161 gcgcccacag ctgtccgccg cgctcggttt cgtagcatgc ggcacggccc cggaaggtca
     5221 ccgcgcacac tttctgctgc tccaccagcg gcgctccggc aatgtcggta atgcgctcca
     5281 gctcgttatc gcccttgggg gccgcgacca ccgtctccca gaccaccccg ccattggcga
     5341 gattcagcac caccagtttg cccgccggca tgccggcgat gacaaaaccc tcgacgatga
     5401 tcacaccggg gttggcgcgc agcgtcagcg gcggcgtgat ggtctggtat tcccagcgcc
     5461 gcgtgccgtc cctcgcattc agtccgaaga tacgactgtc gccgctgcgc accaccacga
     5521 atccatccgc ggcttgcggc gcgctcagca cctcgctcga caccgtggat gtccacaacg
     5581 gtttgccgtc caggtcgtaa gccagcaccg cgcccttggc cgtgccgacc atcaccatgt
     5641 tctcgcccac gcccacgccg cccgaaagcg gcgccttggt atcgacacgc cagacggtct
     5701 tgccggtagc cgcgttcaat cgcacgatct tgccgccact gccggccgca tatacgccgt
     5761 cccggtagaa cgcgggcaca aacaggtaat aacctgcgct gccgacattg gtccgccatt
     5821 ccacgtggag tttcgccgca ggtttaaact ccggcagcgg tgccggcttg ggtcccttgc
     5881 tcccgcaccc gcccagcaga acagcaatca gcgtcgcaat cgcgagccgc agcagcagtg
     5941 actcgaccct cacttggccg gtcccagcgc gtcgagcttg atctgcacca cgctgcggta
     6001 attgctgttg ggcgggctct tttccaaagc caccttgtag gcggcgcgcg cttccgccgc
     6061 cttgccctgt gccgcaagta cgtcgccgcg cagatcggca tagagcggcg caaacgccgg
     6121 ggcgtgtttc gcgtccagca gcgcgagcgc ctcgtcgtac ttcttctcgt cgaggcggat
     6181 gccggccagc cgcaggcgcg ccagcgtcac ggtctcatcg tccctggctt tgtcgatgac
     6241 ccattggagc cgggtcgctg ccgctgccgc gtcgccgctg tcgtaattgc gcctggccag
     6301 cagcagtgcc gccatcggcc cgtaggcagt ggacccatac ttatcgatga tctccgcacc
     6361 caggctgccg atctgcgcaa cgtcgttctt gcgaagcgct tcctcgagtt tgccgaacag
     6421 tgccgaagcc tgctccgact ggccgcgctg gtagtaattc cagcccctca cgccggcaaa
     6481 ggcggccagc cccagaaaag ccagcgtgat gatcagcctg ccgtactgct tccagaactg
     6541 cttgagcgcg tccaactgtt cctgttcttc aaggtcgagt gccatttttg cgaatcttca
     6601 tcatgtagtt gataaattgg ggccgcggat gatgcttgcc gcctcatccg cgctgacctg
     6661 cgcttgctcg ccgggttcgc gcagcggctt gagcgcaatc tgttgttttg ccgcctcgtc
     6721 gtcgccgacg atcaccgcga agcgcgcacc actcgcgtcc gcccgcctca tctgcgactt
     6781 gaaactgccg ccgccgcaat gaaacaccac cgacaagccc ttgccgcgaa gggtctccgc
     6841 cacggtccac gcgaatttgc cggccgcctc accgctgtgc acgaggtaaa cgtcgacgat
     6901 tctcgcgggc gcggccgtgt ccgcatcgat gagcaacgcg agcagccgct ccattcccat
     6961 cgcgaagcca caggcgggag cgggcttgcc gccgatctgc gcgatcaggc cgtcgtagcg
     7021 cccgccggcg cataccgtgc cctgcgcgcc cagcctggtg gtggcccatt cgtagacgac
     7081 gtcattgtaa tagtccaagc cgcgcaccag ccgggtgttg atggcaaaat tgataccggc
     7141 gttgcgcaac aggtcctgca attcctggaa gtgcttcagc gactcctcgt cgaggtcatc
     7201 ggcaagctgc ggcgcttgcg cgatcacttg ctgtatcgcc ggattcttgc tgtcgagcac
     7261 gcgcagcgga ttggtgctca gcctcctgcg tgcatcttcg tcgagttcgg cggcgtggcg
     7321 ttccagatac cggatcagcc gggcgcggta gcgaccacgc gaagcagcgt tgcccagcga
     7381 attgatctcg agccggatgc cgtccagtcc cagccgacgc cacagccccg cactcatcac
     7441 catgtgctcg gcatccacgt ccggccctgc gaatccaagc gcctcgacgc cgacctggtg
     7501 gaactgccga taacgcccct tctgcggacg ctcgtggcgg aacatagggc cgacatagaa
     7561 cagccgctgc ggacccgagt aaagtagatt gtgctggatc gccgcgcgca cgcaggacgc
     7621 ggtgccttcg ggccgcagcg tcaggctctc gccgttcaac tggtcgacga aggtatacat
     7681 ctccttctcg acgatgtcgg tgacttcgcc gatcgcccgt acgaacagtt cggttttctc
     7741 caggatcggc atgcggatat tgcgatagcc atactgcgac agccagtcgc gcaccgtgtc
     7801 ctcgaagaat tcccacagtg gggcctggtc cgggaggatg tcgttcatcc cgcggaccgc
     7861 ctgaatcgat tgcgtcatga aacttgtcgt cgtgcggtag ggcggcgcgc ggcgattagc
     7921 cggtcgcgcg cgccgattca ttgccggggt gtggcgcctg ttcggcagca cacttcccgg
     7981 cgcggccata gtggtccgct acataatcgt ccaccagccg ctggaagtcc tcggcgatgc
     8041 gttcgccttt gagcgtgacg gtcttcaccc cgtccacgaa caccggcgcg accggcgtct
     8101 cgcccgatcc cggcaggctg ataccgatat tggcgtgctt gctttcgccg gggccgttga
     8161 ccacgcaacc cattaccgca acgcgcatct cctcgactcc agggtactgc gcgcgccata
     8221 ccggcatctg ctggcgcaga taaccctgga tcttgtccgc cagctcctgg aagaaggtgc
     8281 tggaggtgcg cccgcatccc gggcaggcag tcaccatcgg ggtgaaggag cgcaggccca
     8341 tggtctgcag gatttcctgc gcgaccacga cttcgcgcgc acgatcgcca ccgggatcgg
     8401 gagtcaggga aacacggatg gtgtcgccga ttccttcctg cagcagcact gcgagtgccg
     8461 ccgccgaggc gacgatgccc ttcgagccca ttcccgcttc ggtcaggccc aggtgcagag
     8521 gatattcgga acgcgccgcg agatcgcgat agaccgtgat caggtcctgc acgccgctca
     8581 ccttgcagga cagtacgatg cggtcgcccg gcaggccaat cgcttcggct ttggccgcgc
     8641 tttccagtgc cgaagcgatc agcgcctcgc gcatcaccgc gctggcatcc ttcggctcgg
     8701 gcaggcgtgc attatcgtcc atcatgcggc ccagcaggtc cggatcgagg ctaccccagt
     8761 tcacgccgat gcgcaccggc ttgccgtatc tgcatgcgca ttcgatcatg gtcgcgaact
     8821 gctcgtcgcg ccgcgagccg cgaccgacgt tgccgggatt gatgcggtat ttgtccaggg
     8881 cctgcgcgca atcgggatgc tgagtcagca gcttgtggcc gttgaagtgg aaatcgccga
     8941 ccagcggaac cgggcacccc aagcgttcca gacgattgcg gatctccgct acggctgctg
     9001 ccgcttccgc gctgttgacg gtgatgcgca ccagctccga accggcctcg gcaagctccg
     9061 ccacctgacg cacggtggct tcgacatccg cggtatcggt attggtcatc gactgcacca
     9121 ccactggtgc gccaccgccg atggccacac cggctacgct tacctggcgg gtgctgcggc
     9181 ggcggattgc gcaatctgac ataggagaaa ccactattcc agcgtcaggc gcgccacgtc
     9241 cacctgggtg tgcgccgaca ggtccacggc ctcgtccttg taggtcaggc gtactccgac
     9301 ggcgttgccg accaccacgt cgaaaggcgg ttcgccgctg acgctgcggc ggctgcccgc
     9361 cgggctgagt tgcccgaaga tggtgttgcc gttgcgatcc ttgatttcca cccaggattc
     9421 ctgatcgaag accatgcgca gagtcgcgcg ctcccccggt tcggcagcca cggcgttgcc
     9481 tgcggccggt tttacggctg cttgtgcagc tatcggtatc gccgccggag cgggttgctt
     9541 cggtagtgca gcctgctcga ctgcggcaga ttcttccgac gccggcttcg agccgccgcg
     9601 gttcccgacc aatacggcaa ccgccgcgac caccaccgca accgccgcga ctgcactcat
     9661 cgccggcacc attccgatcc tgcggcgccg cacgaccagc gggctcgcag ccgcggctac
     9721 cggttcgctc gccggcagcg gcggatggag gacctggtcg gccgcacgga tcaacggatc
     9781 cgggtcaagg ctgagcagct tggcgtaatt gcggatgaat ccgtgtacga acaccggacc
     9841 ggtgaaaccc gagtagtcgt cgcgctcgag tgcttcgacc tggcgcaccg atagcttcag
     9901 ctggcgcgag atatctccga ggctcaatcc ctggcggcgg cgttccatca gcagcgccgc
     9961 acccgcaccg cccggcacgg atgattgtcc gtttgtcgcc gttgcgctca ttgaaaccgc
    10021 ccctcattga atgccttggt ctggacggcg tccggatagc ggttattcaa ctgagtgccg
    10081 tagcccgcca ctgcgttgcg atcaccgaga ctgtcggcaa cgcgcgcgcc cagccataat
    10141 gcgtccggcg aaggattgga cactatctgc agatggcggt cgagcaggtt gcgtgccccc
    10201 tggcgattgc cgcgcttgaa ctccatttcg gcaagctgat acaaggcctg gggctggttc
    10261 ggctgcagcg ccagggcctt gcgcagccaa tcctccgcct ttacatcgtc accctgcatg
    10321 cgcgcgcata cgccggcgtt cgcgtaggcg ttctcagggg tggtgtagag cgggtttttc
    10381 agcgcagcca tgaaatactt gaacgcttcc ttctcgcgct tgcgccggca caggaacatg
    10441 ccgtaattgt tgttcgccgc tgaatcgttc ggatcgatcc ggagcgcccg ttcgaacgac
    10501 tcctgggcca gacggtcctc gccaagcgcc acatacacca tgccgagctg gttgtgcgcg
    10561 ggtccgtagc cggaatcggc cgccagcgcc gccttgaatt cgtcgaccgc ggttttcatc
    10621 tggccggcgc cggcgtagag cgcgccgagt tcggtatgga tgcgggcgcg gttctggatg
    10681 tcggcgcggg tctgttcgtc cgctgcggat ttctgcccct gcgttgcgca accggccagg
    10741 acggccagaa ccacgatcgt caccaaccgg gtcatgttga ggcttccagt gcgcggcgcg
    10801 agcggcgctt ggtgcgatcc tgcacctggc cggcgagctg gccgcaggcg ccatcgatgt
    10861 cggcaccacg cgtcttgcgg atggtcgcca ccagacccgc atgcatcaga actttgcgga
    10921 aggcggcgat gcgttccggg gccgagcgct ggaatccgga gtccgggaac gcattgaacg
    10981 gaatcaggtt gaacttgcag tgcacgccct taacgagcgc gatcagttgc tttgcatgtt
    11041 cgacgccgtc gttgacccca tcgagcatca cgtattcgaa agtgacgaaa tccctgggcg
    11101 ccaccttgat gtagcgtttg catgcggcta tcaattcctt cagggggtat tttttgttaa
    11161 tcggcaccag ccggtcgcgt aactgatcgt cgggcgcgtg cagcgatacc gccagcgcca
    11221 caggcaactc tgcggccagg cggtcaattg ccggcacgat accggaagtg gacagcgtga
    11281 cccggcggcg cgacaggccg taggcgttat cgtcgagcat gattcgcatc gcgctcacca
    11341 cgttgtcgaa attggctagc ggctcgccca tgcccatcat gaccacgttg ctaatgacgc
    11401 gctcgccccg gggatcgtgg ccgagcgcct tgttggccca ccacagctgg ccgatgatct
    11461 cggccaccga aagattgcgg ttgaagccct gacgcccggt ggagcagaac gcgcactcga
    11521 gcgcgcagcc gacctgggac gacacgcaca gcgtaccgcg gtcggtttcg gggatgaaca
    11581 cggtttcgat gccgttgccg gtgccgaccg acagcagcca cttgcgggtg ccgtcggtcg
    11641 cagtggattc cgccatgacc gacggaggct cgaccacggc gcagccatcc agtttggcgc
    11701 gcagatcctt ggcgagatcg ctcatgcgcc cgaactcggc ctcgccgagc tggtggatcc
    11761 agcgcatcac ctggcgggca cgaaacggct tttcgccgat ctcggcaaag agaccggcga
    11821 gccgctcaga gtcgaagtcc agcagattga cgggcatcag cgcgaataaa tgttcagggt
    11881 cgggaagaaa taggcgatct ccaccgcggc ggttcccggt gcgtccgagc cgtgtaccgc
    11941 attggcgtcg atgctatcgg cgaagtcggc gcggatggtg cccttagccg ccttcttcgg
    12001 gtcggtcgcg cccatgaggt cgcgattctt ctggatcgcg ctctcccctt ccagtacttg
    12061 taccatgacc gggccggaga tcatgaagtt caccagatcg ctgaaaaacg gacgctgccg
    12121 gtgcacagcg tagaatcctt ctgcttccgc ccgagacaaa tgcagcattc tggccgcgac
    12181 gaccttcaaa cccttgttct cgaaccggga atagatctgc ccgatcacat tcttcgccac
    12241 ggcatcgggt ttgattatcg aaagggtacg ttcaacagac atcaaaaact ctccagtcgt
    12301 ttggttttta acgacaattc ataaggatag catggccctc gggcgaaagg aatgactttg
    12361 acaggcgcgt cgcgtcgggc gcgccggaaa gcgcgctccg gcggcattcc gggccgcaat
    12421 cgagggacgg agcatcggtg atacgatcgg accgtcgggt gaagcaccaa tcctgaatga
    12481 ccaacaaccc aaacaatgcg cccgccggcg cgcccacccc ggaacgcctg aaacagcgcg
    12541 ccagccggcg cctgctcgcc gcgctcctgc tgatcgctgt ggccatcggc ggattggcgc
    12601 tgacggagcg ctggcgccag ccaccggcga tcactccgcc accccatgaa ccctcgcagg
    12661 cgttgattgc gccgccctcg ccggagccac tcgccgaacc actccagtcc gatcatcctg
    12721 ccgggccccc gcccccgccg caggtcgtca ataacgaaaa gcgggtcgcg cccgcgccgg
    12781 ccaaaccggc tccggcattg cccgcgaccg gcgccaagat tgacgccaag ccggcgccgg
    12841 ctacagacaa gacctacgtg gtgcaggtcg gcgtattcat gtcgcccacc aacgcgcagg
    12901 cgctgcagca acaactggtc aaggccggca ttcccgcgca caccgaaaca cgcgtgcaac
    12961 tcggtccgtt ccaggaccgg cgcgaggcgg agacggctct ggcaaaggtg aaaaaactcg
    13021 gcgtcgacgc ggtgctggtg gcgccgcatt gatatttttc acgaatacag caggccgggc
    13081 agccacagcg agatcaccgg cacgtacgtc accagcgcga ggtaggcaag cagcacgaat
    13141 acccagggca aacacgctat cgatacttcg gtcattccca tcctggcgat atgactcgcg
    13201 acaaacaggt tcaaccctac cggcggatgg atcatgccga tctccatgtt caccgtcatg
    13261 atcacgccga ggtggatggg atcgatgccc aacgacttgg cgataggcag cagcaacggc
    13321 gcaaggatca ggatgatcga gctgggttcc atgaactgcc cggcgaccag cagcgccacg
    13381 ttgaccgcca gcaggaacat ccacggcgat agctgctgat ccaccaccca ctgcgacaac
    13441 acctgcggaa tctgctcgga ggtgagcagg aaagaaaaga ggatggcgtt ggtgatgatg
    13501 tagagcagca tcgcgctggt gttggctgcc gacagcaaca ctttcggcat gtcccgtacc
    13561 ttcagtccgc gatagatgca gaccgccgca atgaaagagt aaaccgccgc gactgccgcc
    13621 gcctcggttg gcgtgaaaat gccgccgtag atccccccca tcacgatcac caccagcatc
    13681 aggccccaga acgcctcctt gaaggacacc cagacttccc gcgccgaggc gcgcggcatg
    13741 gtcgggaagt tctttgcccg cgcggtaacg taggtcacca gcatcagcac gccggccaac
    13801 agaatccccg gcacgacgcc ggccatgaac agcttgcctg cggattccga ggtgctcacc
    13861 gcgtagatga tcatcacgat cgaaggcggt atcaggatgc cgagcgagcc cgacgtggcg
    13921 atcacgccca ccgcgaaacg tttcggatag ccgtgcttga ccattgccgg cagcatgatc
    13981 gaaccgatcg ccaccacggt cgccggactc gaaccggaga tggtggcgaa gaacgcgcag
    14041 gcgaggatcg ccgccatggc cagcccgccg cgcatccagc ccaccagcgc cagcgcgaag
    14101 cggatgatgc gcgccgcaat accgccttcg gtgagcaggg tgccggaaag cacgaagaac
    14161 ggcaccgcca tgatcgcgaa actttccagc ccggtgaaca agcgctgcga cacgatgtcc
    14221 accgtgccca gggcgaagga ggaaaagagc gcaaggaaag tgagaaccgt catccccagc
    14281 gcgatcgaaa tcggcgtgcc ggtcagcagc agcaggagca ggatgaacag aatgagggcg
    14341 acgttcacgc cccctcctcc gcggcggccc cgtgggcctg ggccggcagc acgccggatt
    14401 tgacgaagcg cgccatcacc tgcaggaagc gatagcacat cagcgccgag cccaacggga
    14461 tgcatagata gaccacccac atcggccact ccaggtccgg cgacacctgt ccggtgccgt
    14521 ggatgaacca aacccagcgc gcccccatga tggcgatgac cgcggtgaag aaggccccca
    14581 gcgacagcgc caggaccacc agcacgcgct tcagccgcgg accggcggcg ttgaccgcga
    14641 catcgacgcc gatgtggatg ccggtgcgca ctccgtaggc ggcgccgaat ttcgccatcc
    14701 agatgaacag ataaatgcaa agttcctgcg cccaggaaaa attcagcgcc ccggtgtagt
    14761 cccacaacca tgggatggcg gacgattgcc tatggagaac cgcggcaaat atgatgagcg
    14821 tggccgccgc catgaacgag gcgaccagcc attcctcgat gcgatcgagg acgcgattga
    14881 agatgcggcc caccaggctt actttttctt ggctttttct gcttcgactg cggcggcgac
    14941 gcggtagatc gcctgcagat tgtccctgcc gaccgtctgc tcgaacttcg cgtgcaccgg
    15001 cagcagcgct tcacgccagc gtttgcgctg cgcatcgccg agcacgtaga cctcggtctt
    15061 cttcgatgcc ctcactgccg cgagcgcgtc ctcattgtcc ttcttggcga cgtcgcgctc
    15121 gtaaacggtg gtttcccgca tcgccttctc cagctgggcg cggacatcgg cgggcagtcc
    15181 gtcccagaac ttcctgttta cgatcaccgc gtacaccagg cagccgtgat cggaaatcgt
    15241 cagatgcgac tgcacctcgt gcatcttctg cgtatagagg ttggagaccg gattctcggt
    15301 accgtccacc acgcgctgct gcagggccga atagacctcg gagaacgcca tcacctgcgg
    15361 cagcgcgccg agctggcgca tcatcgcctc cagcaccttg gaagactgga tgcgcatctt
    15421 caggccgcgg aaatccccgg gttcccgcag cgggcggttg gcggagaact gcttgaaacc
    15481 gttgtcccag tacgccaggc ccctgatacc gcgggattca agcttcgcga acagggcctg
    15541 gccgatctcg ccgtcggtga cgcgatccac ggtttcggcg tcggggaaca gatagggaag
    15601 gtcgaacacc tcgaactcgc gcacgcccag cgggccgaac ttcgacaccg acggcgccag
    15661 catctgcacc gcgccgagct gcaatgcctc catctcctcg cgatccttgt acagctggct
    15721 gttgggatag agttcgacct tgacccggcc cgaactgtac ttttcggcga gctgcttgaa
    15781 ttttgccgcc gcctcgccct tgggcgtatc ggtcgccacc acgtggctga acttgatgac
    15841 aatcggatcg cctgcgaagg cggaaatcgt tgtggcaaac aacagcgcag cgaaaaccag
    15901 tctgatcatt tttgtacgag gcccggatac ccggcctttc tcctcggttt attctatcag
    15961 cgcttccact ccggcacgat gccctgctcg ccgcttgcgg cgcgaaactc cgcggccacg
    16021 ccgatgccgg gcacccaggc cagcgcatcg ccgatgaata gcagcggcat gcgttcgcgt
    16081 tcccaaggag gcaccgcggt ttcccgcaat aagttcttca gcgtgcggcg cggacgatcc
    16141 tccgccggcc gcatgcgctc gccgccagtg cgaccgcgca cggtcgcgcc ctgtcgcgcg
    16201 aaatgacgcg cggcaatgcc ttcgccgagt gctgctcgcg cgcgcagcac ccccagcccg
    16261 gcgggcaaca gcaactccct gcggccgtcc cattgcgtgt tccagtcgac gggcatctcg
    16321 ccggattcct cgacgattgc gacctggccc cggaagcaac gcaagccgtg gctgccgaag
    16381 gacacgtgca cttcagagtc cgcgccggcc gcgcggcact gtcgcaatga ctcctcgagt
    16441 cttgtcctgt tgggcatcgg cagcgcgcgg ctcgcaaaca gccggcgcag aacattcagc
    16501 gcgcgcgcat cggaaagccc gcgcaggctc tcaacgggaa tcaccccgtc ctcaaacgcg
    16561 cgcgcgtcga acgcagccag ttcttcagca agctccgaat aatcagccag attgcggctg
    16621 gcgcgcgaaa gcgtctcgcg ataggcggga aagcgcgcct ccaggcgagg cagcaactcg
    16681 ttgcgcagaa aattgcggtc atagcgcgaa tctgcattgc tgtcgtcctc gatccactcc
    16741 agtttgcgcg tctttgcata tgcttcgatc tcgccgcgcg tcacctcgag cagcggacgc
    16801 aaaatgcaag gatgcgagac caaggatgaa ggcggaagga tgaaggatga aggatgagaa
    16861 tccaaggatg aaggatgagg gatgagggat gaagtgtcgt gttgcgaagc aatttccctt
    16921 ccttccgcct tcatccttcc gccttcatcc ctgcccttac cccttccgcc ctcatccttg
    16981 cccttctccc ttccgccttc atccttgctt gtaccgtctt catacttgcg cagtaccggc
    17041 atcgccgcca ggcccttggc tccggcgcct cgcagcaatt gcagcagaaa cgtctccacc
    17101 tggtcatcga gatggtgggc cagtgcgacg aagtcggcct cgagcccgcc gtatgcctca
    17161 tatcgcaatt tgcgcgccgc cgcctctacg ccgctgccgt cacggggcac cctcactcgg
    17221 cgcacggtca acgcaacgcc gcgcgcctgg cacagcgtct tggcgaacgc ctcccattcc
    17281 tgcgcatgcg cgctgatctg gtgattgacg tggaccgccg acaacgccag ccgcaagtcc
    17341 ttgcgcaacc gcccgagcac gtcgagcaac accacggaat ccaccccgcc gctcagaccc
    17401 acggcgacgc gccggccgcg caccgcgtgc gtattcagca ctgctgcgac agtctccagg
    17461 agccgttcgg tcatgatcgc gaaaataata aagtcacgag ctcaaaacaa gataccccgc
    17521 cgaggcgggg tatcggggac gaatcgcgcc gcgcgggccc gctcaacgaa ccgcgacctc
    17581 cttgaatttg ccgtaggcca tcaggcgtcc gaatcgcgcc tcgaccagtt cgtcgatcga
    17641 tttccgctgc gcttccgcga gcgcctcctg caacgccttg cgcagcgtct gcatcatggt
    17701 cgccgggtcg cggtgcgcgc cgccgagagg ctcagtgacc acgctgtcca ccaggcccag
    17761 cgttttcagc cggttggccg tgatgccaag cgtctcggcc gcgtccggcg ccttgtccgc
    17821 gcttttccac aggatcgagg cgcagccctc gggtgaaatc accgaataga tcgaatactg
    17881 cagcatcagc gtcacatcgc ccaccgcaat cgccagcgcg ccgccggatc cgccttcacc
    17941 gatgatggtg acgaccactg gcacgcgcaa ttccgccatc acatacagat tgcgcccgat
    18001 cgcctcagac tgcccgcgct cctcggcgcc gaggcccggg taggcgcccg gcgtgtccac
    18061 gaaagtcatc accgggatac cgaatttttc cgccagcctc atcagccgca gcgcctttcg
    18121 gtatccctcc ggcttgggca tcccgaaatt tcggtagagc ttgtccttgg tgtcgcggcc
    18181 cttctgatgg ccgatcacca tcaccgtctg gctgccgaag cgcgccaggc cgccgatgat
    18241 cgaaggatcc tccgcaaagg cacgatcgcc atgcaactcc tcgaagtcgg taaagttgtt
    18301 ctggaaatag tcgagcgagt agggacgctg cgggtggcgc gccacctgcg aaatctgcca
    18361 ggcggaaagc ttggagtaga tctccctggt gagcgcgctg cttttcttct gcaggcgtgc
    18421 aatttcctcc gatatgtcca cggcggagtc gtcctgcacg taacgcaact cttcgatctt
    18481 cgactccagc tcggcgatcg gctgttcgaa ttctaggaag gtggttttca actgggccat
    18541 tgattatggt ggcgccatgt tcgtcgcggc gccaaattag tagcggcgcc gtattgtagc
    18601 ttgtcgcgtg cgtcccggct ttacttgccg tactggcccg ccagataatc cacgattttc
    18661 tgcgcgtccg cttccgcgat cggcgcgccc atcaccttga tcatcttgtt gaccgaggcc
    18721 tcccagccct tgcggtcgag aaacggcgaa ttcatcggaa tgtaatcgag gctgtggcac
    18781 atgacgcagc tggcctccac cagttgcctg ccgggcccat ccctgagccg caactggctc
    18841 tcctgtgcca gcgccggcgc agcggcaaga accatcatca gcgcgagtat cgtgcgcata
    18901 ctcaactcct acgtgatttc gatattgatc cgctgcacca cgttgttgtg gtagccggcg
    18961 gggttgaaga tcagttcgaa agtctgcgtg gcccccgctc gattcgccgc cctggccatg
    19021 agcatatggc tgccggcacg cccgggcacg aagcgatgcg accactggcg ccaggagaat
    19081 cttcccagat cggtggcgag ttgcgccgcg cgccaggtcc tgcccccgtc ctcggagact
    19141 tcgacggttg cgatcccgaa tccgccgtcc caggccacac cggccaccgt gatctcctgc
    19201 ccgaaagcaa acttctgccc gtcttcgaga ttggtgacca gggaattcac aaccatttcc
    19261 gtgatcggcg tattgacctc ggtttcctgc gacacgaaac gatcgaccag cggaaacttg
    19321 cccttgggga tgcggtacgc cggattcatc cagaacccct ggtagggctg cgcggtcgcc
    19381 gtaatcgagg tgagatgctt catccaatac gtcgcggtcc agccaggcac cacgatgcgg
    19441 acgggaaaac cgttccagtg cggcagcttt tccccgttca tctccagtgc cacaagggtg
    19501 ttctcgtcca tcgccttcca tgccggaatg ctttttacga aatccggggt cttgtcgagc
    19561 acacccctgt cggtgccatc gaatacgatt tcgagcgcgt ctttcttcac gccggctttg
    19621 gccagcagat cgcgcagcct tacgccacgc cacctggcat tgccgatcgc gccgtggccc
    19681 cattgcacgc cgggcacgtg cggctgcgac aggccgcgcc ggttgccgga gcacatgcac
    19741 accgcagcga cttcgacttg ctcgaagtcg cgggacagtt cctcgtaggt gatctcgaac
    19801 ggcttgtcca gcgcgtcgcc cgagacagcg agtttccagc gctgctggct gacctgcgga
    19861 atgtcggcaa ggtggtagcg cacgaaaaag gcgttgttcg gcgtgaacgc ctcgttgaag
    19921 aaacgcaccg gggtttcgaa gttcggcggc cggaaggatt tcttgatcag tggcagcttg
    19981 cccggcagcg ccgcaagttg cgccgattgc agtgcgcccg acggcagtgc cgcagggccg
    20041 aggctgtccg ccgccactgc cgaatcggct ccgcgtatcc cgccggcgag cagcaggccg
    20101 cccgccgaaa ctttcaggaa atcacgcctg gtttgcatgc ctcctccaac tctggtttgc
    20161 ccgcccgcgt tcaaaacaat acgccggcct ggccgcgcgg gtcgttggcg ctggtgacgc
    20221 gcccggtttt cctgtcgaaa tacactgcct gcatgttacc ccacttgcgc cgcccctctt
    20281 ccagcgcatg ccccttggcc ctgagcgccg caatccactg ctgcgaaaat gcgcccggct
    20341 cgatttcgat gcgatcgggt agatactggt ggtgatagcg cggcgcgccg accatgcgct
    20401 gcaggtcgaa cagtggttga tccacatagg cgagaattcc ctccagcacc atcgagatga
    20461 tgcgcgcccc cccaggagtg ccgagcacca gcacgccgcg ctcatcctcg acaaaggtgg
    20521 gcgacatcga cgacaggggc cgcttgcccg cctcgatcgc gttggcctcg ttgccgacca
    20581 gcctgtagat attcgcggcg gacggtgcca gggagaagtc gttcatgtgg ttgttgagca
    20641 gcaccccggt gtcgccggcg acgaaacccg atccgaacgg cgcattcacc gaaagcgtcg
    20701 ctgcaacgcg attgccctcg gcgtcgatta ccgacaaatg cgtggtgtcc ttgccctctg
    20761 cggccgcatc cgctatttcg gcgagcgcag cgctgggcgt ggcgcgatcg ggatcgatgg
    20821 atgcggcgcg ttgttttgca tactcccgcg aagccagttt ctcgactggc acagccacga
    20881 agtcggggtc tcccatgtag cgggcgcggt cgtggtagcc acgccgcatt gcctctaccg
    20941 tgtagtgcac gcggtcggtc tcggacaagg tagccagtgc cacccgttcg agaataagca
    21001 ggctctgagc cagcaccagc ccgcccgaag acggcagcgg cgcggaggtg atggtcgcgc
    21061 cgcggtagct gaacttggcc ggcggccgct cgaccacgcg atagctttcc aggtcggaga
    21121 gttcccacaa cccgccgcct gcgcgcaccg cctcaaccag ccggcgggcg aagtcgccgc
    21181 gataaaagcc ctggcgccct tgcgccgcga gcatgcgcag cgtgcgcgcc agttgcggct
    21241 gtttcactac gaagcctggg ccaaccgcgt tgccgttgtc gagaaatatt cgccctgcgt
    21301 tcacgtccgc cttcaacatt gcctcgcgat aacccgtggc ccagacgtag cgcgcatcgg
    21361 cggggaaacc ctgctcggca agacggatcg ccggctgcaa cgcacgcgcc agcgtcaact
    21421 ttccgtagtg cccggcgacc caggcgagcg ccgccggcat gcccgggatg cccgcggcgg
    21481 tggggccttc gagcgaagcc ttctgtctcg gttggccgtt gtcgtcaagg tagaactcgc
    21541 gggtggcttt ggccggcgcc gtttcacgcg catcgacgaa cacgtcgaaa gcgtcggcgc
    21601 cacgatgcag cagaaagaat ccgccgccgc cgatgcccga cgcatacggc tcgaccaccg
    21661 ccagcgcggc ggccaccgcc accgcagcat cgaacgcatt gccgccggat ttcaggatgt
    21721 cgcaacccgc acgggtggcg agggtgtgtg cggaggcgat gccgcattcc tgcgccgctg
    21781 cgggaccctg cgccaacccc gccaacagtg cgacgacaaa cgcaaaccgt agtaccgcac
    21841 cggaccaggg ccgtttcata tcagatccgc gtctcatcca ggcacgccca tttcacgtag
    21901 tcacttcctc aagaaacggc cggaacggat aggcctccgc aatgccgcga aacttcctca
    21961 gaaaatcgcc gcgcatcggc tgcaagccgt tgtctccctg gaagttggcg gtgaaggcgc
    22021 cggggaactc gcgccgcatc atcgaactca cctccctgat gtgtgcgtcc gtatccat
//
LOCUS       DASQOP010000088        12962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_635495, whole genome shotgun sequence.
ACCESSION   DASQOP010000088 DASQOP010000000
VERSION     DASQOP010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12962
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635495"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1889
                     /gene="nuoL"
                     /locus_tag="VE030_03470"
     CDS             <1..1889
                     /gene="nuoL"
                     /locus_tag="VE030_03470"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HZF22509.1"
                     /translation="IGRRATHWVAIIGMTVSTVAAFLVFRDVLAGNAFNGPVYTWITT
                     GDISFEIGFLIDRLSATMMLVVTFVSLMVHIYTIGYMADDPGYQRFFSYISLFTFSML
                     MLVMSNNFLQLFFGWEAVGLVSYLLIGFWYTRPSAIYASLKAFLVNRVGDFGFLLGIA
                     LIFVYCGSLDYAQVFSRAPELTGETVEMAFVGWPLLTTVCILLFIGAMGKSAQFPLHV
                     WLPDSMEGPTPISALIHAATMVTAGIFMVARMSPLFELSETALSFVMVIGAITALFMA
                     LVAVVQNDIKRVVAYSTLSQLGYMTVALGASAYAGAIFHLVTHAFFKALLFLGAGSVI
                     IALHHEQDMRKMGGLGKYMPITYATMLIGALASAGVPGFSGFFSKDAIIEAVHLSQLP
                     GSGFAHFCVLSCVFVTAFYTFRLFFMTFHGKERFQVHGDDQGHGHGHAPHESPWVVTV
                     PLVMLALPAICAGWVIGAVLFGGFFGDAIVVAETHDVLAELGQDFHGATAMMAHALAT
                     WPLWLAVAGIASAAYLYLKRTDLPDKLQHWFSPLYTVLDQKYYFDRFNDWFFAGGARL
                     VGRGLWKGGDVAVIDGFVNGSARLVGWLASVIRKLQSGYVYTYAFAMIIGMVTLLTLW
                     FARS"
     gene            1889..3370
                     /locus_tag="VE030_03475"
     CDS             1889..3370
                     /locus_tag="VE030_03475"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HZF22510.1"
                     /translation="MFSGYQLSTVIWLPIVAGVAVLFTGSDRNAHVARWLALAGAVGD
                     LAVALPLYAGFDPGAAGMQFVELLPWIESYNVNYHLGIDGISLLMILLNCFTTMLVVL
                     AGWQVVGNRVAQYMAAFLILSGIMNGVFCAQDAALFYVFFEATLIPMFIVIGVWGGPN
                     RVYAALKFFLYTLVGSLLTLVAFIYLYVASEGSFALADYYRLPIPLGTQILLFIAFFA
                     AFAVKVPMFPVHTWLPDAHVEAPTGGSVVLAAVMLKLGAYGFIRLSLPILPDASHVAA
                     PVVIGLSLIAVAYIGLVALVQTDMKKLIAYSSISHMGFVTLGIFLFNDHAIEGAVIQM
                     VSHGFVSGALFLCVGVLYDRMHSRLIADYGGVVNRMPVFAAFFVLFAMANSGLPGTSG
                     FVGEFLVILGTMEENFWYAFAAASTLLFGAAYTLWMIKRVVFGPVANGRVAALTDITL
                     REGLVLGLLAIAVLAMGLYPQPFAAVLHTSVNELLGLANQSKL"
     gene            3515..4966
                     /gene="nuoN"
                     /locus_tag="VE030_03480"
     CDS             3515..4966
                     /gene="nuoN"
                     /locus_tag="VE030_03480"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HZF22511.1"
                     /translation="MEYEIPDFLLAAPEIVLLTMVCVILIVDLFLSDAHRVWTYALSQ
                     LSLIGTAVLVFVMGGEDVAYTFNGMFVDDPMADVLKIAVCLTVAVMLAYSRAYIAARD
                     MYRGEFFVLSLFATLGMMVMISANHFLVLYLGLELLSLSLYAMVALPRDSGNSTEAAM
                     KYFVLGALASGMLLYGMSMIYGATGSLEVTEVAQRIFDGDAIPAILIFGLVFVVAGIG
                     FKLGAVPFHMWVPDVYHGAPTAVTLLIGTAPKLAAFAFIMRLLVEALGMQQLLVEWQQ
                     MLVLLAVASLAIGNITAIAQTNLKRMLAYSTISHMGFLLLGILSGTLEGFGAAMYYTV
                     IYVLMALGTFGMILLLSRQGFEAENLEDFKGLNQRDPWYAFIMMLLMFSMAGIPPTMG
                     FWAKLFVLQAVLSVGYVWLAVVAVLFSLIGAFYYLRVVKLMYFDAPTDAAPIDPRADI
                     RALMSANGLAVLVFGLAPGSLLAICERAIQLSL"
     gene            4963..5511
                     /locus_tag="VE030_03485"
     CDS             4963..5511
                     /locus_tag="VE030_03485"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZF22512.1"
                     /translation="MSTDFTEKKISSRTAYRGRLLQVNEDEVRLPDGSHAQREYILHP
                     GAALILPIFDDGSVLLERQFRYPVGCHFYELPAGKLEPNEPPLETARRELQEETGYVA
                     AEWRELGRLHPCVGYSNETIDFFLARKLEFKGAQLDQGEFLETLRLPLAEGQDWVRRG
                     RISDTKTILGLFWAEKILREGW"
     gene            complement(5525..7540)
                     /locus_tag="VE030_03490"
     CDS             complement(5525..7540)
                     /locus_tag="VE030_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZF22513.1"
                     /translation="MRRHVGFAGPTNRQQWSARRAMAHELPEYSIQEAAAKLGIPVQK
                     LRRWDAQGVLVARRTDGGHRRYAREIIDGLAGSALVAGSDKHEAEKYVDELAAARRTL
                     KEKRRIIQLLLESESRYRDLVETSHDLIWTTDALGRFTYLNNGCVDIFGLQQKDLIGR
                     CFFDFEAGLSHVSNRRFLSALRKNGEVKNYLTHLISADGNDRWVGINARVSHDDNGAI
                     IGLRGTARNVTEQHKAALEIERFATHDTLTGLPNRVSLQKVLEAALADGEKGSVLLLD
                     LDHFKYINDNFGHRAGDQLIIGIGGLLKDVVKDENAQVFRLGGDEFALHLPDALRAEA
                     AQVAERALTGLRHYKFQLGDQGCLSTLTASAGVVSYPFHGNDVASLLANVDIAMYQAK
                     DNGRNRVVLHDHNPDDLRSTHRRVHWARELREVLDEDRVLLYSQPVVRLADQTLVHHE
                     VLVRIVDRSGNIVLPGQFIEVAESLGMAQEIDLRVVTKVLEVLKQPQMRGQKMRYFVN
                     LSRVSLSDQHWVQRFQGMLAAAPVDHEQLVFEITETAAMAEVGVTQEFISQMKKLGCR
                     FALDDFGAGFSSFYFLKRFEVDYLKIDGNFIRGLASDEANRVFVRALCDVARGLDKQV
                     IAEWVENQEVLKILTEMGVQYGQGFVFGHPQPFPASASADRQAAKLA"
     gene            complement(7540..8496)
                     /locus_tag="VE030_03495"
     CDS             complement(7540..8496)
                     /locus_tag="VE030_03495"
                     /inference="COORDINATES: protein motif:HMM:NF006452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HZF22514.1"
                     /translation="MDMNIPQEMLKVPAATGAEPAREATEYFFSEQLSAFFDGEQHAM
                     WLRWNPTPRPNFNPDLLRDLDRYCRFITHTGGEVNCGGESAPVEFAILASKVPGVFNL
                     GGDLGLFTRLINSQDRDGLLGYGKACVDVLYRNYIGHELPITTISLIQGECLGGGFEA
                     ALSSDVLVAERQARFGFPEIMFNLFPGMGAYSFLERKVGKRPTENLITSGKIYSADDM
                     LAMGVVDVVVDQGRGEAEIASFIRSRGRSRNAMAGIAAARRRVHKLDYEELMGVVEVW
                     VDTALNLSHRDLKLMQRLVSRQNDFTTTGARRDEAGVAKHLH"
     gene            8834..11035
                     /locus_tag="VE030_03500"
     CDS             8834..11035
                     /locus_tag="VE030_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZF22515.1"
                     /translation="MHFLLRSIVRLLFRVRVVGDLAPFGHADRLLLVSNHHSRLDAVL
                     LALFLPRAPVIVVPPEETRSRLMRWLLGHVDHEVHDLNNPISMKPLLRLLRGGRPVVL
                     FPEGRIARNAAVMKVYPVPALIALKSGATVLPVHVEGAGQWFSRSGSTSVMRRLLPSM
                     TVRVFTPTRIDGGAAGSARRRRAFATRRLTSIMQTMALQSHACKPLFECFLDAAAVHG
                     RGAVLMEDQNEDPQTYGKLLRMSLALSRLLKRFTSAGENVGVLLPNVVPAVGVVMGLS
                     ATGRVPAIFNYAAGPLGVESARVAAGVRTVITSRKFIEQGHLHSLLDALAGCNIVYLE
                     DLRSGFGWRDKLWLIGLALRFPRLVIARQSTLDPAVVLFTSGSEDRPKGVVLSHHAIV
                     ANVAQMRAVFDFSPEDKILNPLPLYHAYSFTAGVMLSLITGTRLYLFISPLRYRAIPE
                     IAYRRDCTVLFGTSTFLSFYARYANPMDFCRLRYVISGGEKLSPEVSRVWMEKFGLRI
                     LEGYGSTECAPVISLATPSAYRRNAVGRFLPGIDHQIEPMDGIDKGGVLHIRGPNLML
                     GYYRYANPGVIEAPKSSVGEGWYDTGDVVELDHDGMLSISGRVKRFAKIAGEMVSLDM
                     IEHVAREASPGHHHAAVLTMQDFAGESTVLFTTDAQLTRAQLLEAANELGTHDLSVAR
                     RIVHLREIPLLASGKVDYVGLKSLIEGDAVRKLLAAMARNPLDIESRPAAK"
     gene            11262..>12962
                     /gene="htpG"
                     /locus_tag="VE030_03505"
     CDS             11262..>12962
                     /gene="htpG"
                     /locus_tag="VE030_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HZF22516.1"
                     /translation="MAEAAGKQTLGFQTEVKQLLNLMIHSLYSNKEIFLRELISNASD
                     ACDKLRFEALTDPGLFEADPDLRIRVWYDKAARALTISDNGIGMSRQEVIDNIGTIAK
                     SGTREFFDRLTGDQAGDAHLIGQFGVGFYSSFIVADKVTLVTRRAGLGKEHGVRWESS
                     GEGDYTIETVEQQGRGTDVTLHLRMGEDEPLDGFRLRSIMRKYSDHITLPILMKKEEW
                     SQEKGQNVVKDEDEKVNQASALWARPKSEISEEQYQEFYKHVAHDFEPPLAWVHARVE
                     GKSEYTQLLYLPARAPFDLWDREHRHGIKLYVRRVFIMDDAEQLIPAYLRFVRGVVDS
                     NDLPLNVSREILQESKDVEVIRKGCVNKVLGLLEDLAENQKEKYARFWKEFGHVFKEG
                     TGEDGANRERLARLLRFASTHGDSDGQTISLTDYLSRMKEGQDKIYYITADGLAAAKN
                     SPHLEIFRKKGIEVLLLSDRVDEWVASNLTEFEGKPLQSVAKGDLDLGKLADEEKKEQ
                     EKEAGEYKDLVEKIRKALDARVKEVRLTFRLTSSPACLVSDQNGMSANLERMLKAAGQ
                     K"
BASE COUNT         2255 a         3966 c         4007 g         2734 t
ORIGIN      
        1 tgatcggccg gcgcgccacg cactgggtgg cgatcatcgg aatgacggtg tcgacggttg
       61 cggcctttct ggtgtttcgc gacgtgctcg ccggcaatgc attcaacggc ccggtttata
      121 cctggatcac caccggcgac atctctttcg aaattggatt ccttatcgac agattgtcgg
      181 ccaccatgat gctggtggtg acctttgtct cgctgatggt gcacatctac accatcggct
      241 acatggcgga cgatcccggt taccaacgct tcttttcgta tatttccctt ttcaccttct
      301 cgatgctgat gctggtgatg agcaacaatt tcctgcagct tttcttcggc tgggaggcgg
      361 tgggcctggt ttcctatctg ctgatcggat tctggtacac acgcccgagc gcgatctatg
      421 ccagcctcaa ggctttcctg gtcaatcgag tcggtgattt cggttttctg ctcggcattg
      481 cgctgatatt cgtgtactgc ggctcgctcg attacgcgca ggttttctcc cgcgcacccg
      541 aactgaccgg cgaaacggtg gaaatggcat tcgtcggctg gccgctgctc accacggtct
      601 gcatcctgct gttcatcggt gcgatgggga aatcggcgca gtttccgctg cacgtctggc
      661 taccggactc gatggagggt ccgacgccga tctcggcgct gatccatgcc gccaccatgg
      721 tgaccgcagg cattttcatg gtggcgcgca tgtcgccgct gttcgagcta tcggaaacgg
      781 cgctgtcgtt cgtgatggtg atcggcgcga tcacagcttt gttcatggca ctggtagcgg
      841 tggtgcagaa cgacatcaaa cgggtggtgg cgtactcgac cctgtcgcag cttggttaca
      901 tgaccgtggc actcggcgcg tcggcctacg ccggggccat attccacctg gtaacgcatg
      961 ctttcttcaa ggcgctgctg ttcctcggcg ccggctcggt aatcatcgcc ctgcaccacg
     1021 aacaggacat gcgcaagatg gggggcttgg ggaagtacat gccgatcacc tacgccacca
     1081 tgctgatcgg cgcgctggca agcgcgggcg tccccggatt ctccggtttc ttctccaagg
     1141 atgcgatcat cgaggcggtg cacctgtcgc agttgccggg cagcggtttt gcccacttct
     1201 gcgtactgtc gtgcgtgttc gtcaccgcgt tctatacctt ccggctgttt ttcatgactt
     1261 tccacggcaa ggagcgtttc caggttcacg gcgacgatca gggacacggg cacggccacg
     1321 caccccatga atcgccgtgg gtggtgaccg tgccgctggt tatgctggcg ctgccggcga
     1381 tctgtgccgg ctgggtcatc ggggccgtat tgttcggtgg tttcttcggc gatgccatag
     1441 tggttgccga aacccacgac gtgcttgccg aactgggcca ggatttccac ggcgcgacgg
     1501 cgatgatggc gcacgcgctg gccacctggc cgctttggct tgccgttgcc ggcattgcca
     1561 gtgcggctta cctgtatctc aagcgcaccg atctgcctga caagttgcag cactggttct
     1621 caccgcttta taccgtgctc gaccagaaat attatttcga ccgcttcaac gactggttct
     1681 tcgccggcgg tgcgcgcctg gtcgggcgcg gattgtggaa gggcggcgac gtcgcggtga
     1741 tcgacggttt cgtcaacggc tccgcaagac tggtcggatg gctggcgagc gtaattcgca
     1801 agctccagtc cggatatgtc tatacctacg cattcgccat gatcatcggc atggttacgc
     1861 tgcttaccct ctggttcgca cgctcctgat gttttccggg tatcaactca gcaccgtcat
     1921 ctggctgccg atcgttgccg gagtcgcggt gttgtttacc ggctccgacc gcaatgccca
     1981 tgtcgcccgc tggctggcgc tcgccggtgc cgtcggggac ctggcggtcg cgctgcctct
     2041 ctacgccgga ttcgaccccg gcgccgccgg aatgcagttc gtggagttgc taccctggat
     2101 cgagtcctac aacgtcaact atcacctcgg tatcgacggc atctcgctat tgatgatctt
     2161 gctcaactgt ttcaccacca tgctggtggt gctggcggga tggcaagtgg tgggaaaccg
     2221 ggtggcccag tacatggcgg ccttcctgat cctgtccggg ataatgaatg gcgtgttctg
     2281 tgcgcaggat gccgcgctct tttatgtgtt tttcgaggcg acgctgattc cgatgttcat
     2341 cgtcatcggc gtgtggggag gcccgaaccg ggtttatgcg gcactcaagt tcttcctcta
     2401 cacgctggtc ggctcgctgc tgacgctggt tgcgttcatt tatctgtacg ttgcctccga
     2461 gggcagtttc gccctggccg actactaccg tctgccgatc cctctgggga cgcagattct
     2521 gctgttcatt gctttctttg ccgcgtttgc ggtcaaggtg ccgatgttcc cggtgcatac
     2581 ctggctgccc gacgcgcacg tcgaggcgcc caccggcggc tccgtggtgc tggcggccgt
     2641 gatgctgaag ctcggcgcct atggcttcat ccggctgtcg ctgccgatac tcccggacgc
     2701 cagccacgtg gcggccccgg tcgtcatcgg cttgtccctg attgcggtgg cgtacatcgg
     2761 gctggtggcg ctggtgcaga ccgacatgaa gaagctgatt gcctactcct cgatttcgca
     2821 catgggattc gtgacgctgg gcattttcct gttcaacgac cacgccatcg aaggcgcagt
     2881 gatccagatg gtctcgcacg gattcgtttc cggtgcgctg ttcctgtgcg tcggcgtcct
     2941 ctatgatcgc atgcactcgc gtttgatagc ggactacggc ggggtggtga accgtatgcc
     3001 ggtatttgcc gctttcttcg tgctgttcgc gatggccaat tccggtttgc cggggaccag
     3061 cggctttgtc ggcgaattcc tggtgatact gggaacgatg gaggagaact tctggtacgc
     3121 gttcgcagcg gcgagcacgt tgctgttcgg cgccgcctat acgttgtgga tgatcaagcg
     3181 cgttgttttc ggcccggtgg ccaacggccg ggttgcggct ttgacggaca tcaccctgcg
     3241 ggaggggctg gttctgggcc tcctcgcgat tgcggtgctg gcgatgggac tgtatccgca
     3301 gcccttcgcc gcggtgctgc atacttcggt caacgaactg ctggggctgg caaaccagag
     3361 caaattatga aaactggggc gtgggacgag ggacgaggga cgagggaagc ggaaaatcgt
     3421 gcggttgcgc tttatgcttg tccctcgtcc ctcgtctctc gcccctcgcc cctcggttca
     3481 cggccctcgc ccctcgcccc tcgtccctga atctatggaa tacgagattc ccgattttct
     3541 gctggcggcc cccgagatcg tcctgttgac gatggtctgc gtcattttga tcgtggacct
     3601 gtttctgtcc gatgcccatc gcgtctggac ctatgcgctg tcccaactgt cgctgatcgg
     3661 caccgcggtg ctggtgttcg tcatgggcgg ggaagacgtc gcctacactt tcaacggcat
     3721 gttcgtggac gacccgatgg cggacgtgct caagatcgcg gtttgcctga cggtggcggt
     3781 catgctggcc tactcgcgcg cctacattgc cgcgcgcgac atgtaccgcg gcgagttctt
     3841 cgtgctttcg ctgttcgcca cgctcggcat gatggtgatg atctcggcga atcacttcct
     3901 ggtgctctat ctggggctgg agctgctgtc gctgtccttg tatgccatgg tggcgctgcc
     3961 gcgcgattcc ggcaacagca ccgaggcggc gatgaaatac ttcgtgctcg gcgcgctggc
     4021 ttccggcatg ctgctctacg gcatgtcgat gatctacggc gccaccgggt cgctggaggt
     4081 caccgaggtg gcgcagcgca ttttcgacgg cgatgccatt ccggccatcc tgattttcgg
     4141 gctggtgttc gtggtcgccg gcatcggttt caagctgggt gcagtgccgt tccacatgtg
     4201 ggtgccggac gtctatcacg gtgcgccgac ggcggtgaca ctgctgatcg gaaccgcgcc
     4261 caagctggca gcgttcgcgt tcatcatgcg cctgctggtc gaggcgctgg gcatgcagca
     4321 attgctcgtc gaatggcagc aaatgctggt gctcctggcg gtggcctctc tcgcgatcgg
     4381 caacatcacc gctatcgcgc agaccaactt gaagcgcatg ctggcctatt cgaccatttc
     4441 gcacatgggt ttcctgctgc tcggcatcct cagcggcacg ctcgagggat tcggcgcggc
     4501 catgtactac accgttattt acgtgctgat ggcactgggc acatttggca tgatcctgct
     4561 gctgtcgcgc cagggcttcg aggcggagaa cctcgaggac ttcaagggac tcaaccaacg
     4621 cgacccctgg tacgccttca tcatgatgct gttgatgttc tcgatggcgg gcatcccgcc
     4681 caccatgggg ttctgggcca agttgttcgt gctccaggcg gtgctgtcgg tgggttacgt
     4741 ctggctcgcc gtggtggccg tgctgttctc gctcatcggc gcgttctact atctgcgggt
     4801 ggtcaagctg atgtacttcg atgcgccgac cgacgcggcg ccgatcgacc cgcgcgccga
     4861 catccgggca ttgatgtcgg ccaacggcct tgcggtgctg gtgtttggat tggcgccggg
     4921 ctcgctcctg gccatttgcg aacgcgcaat tcagctttcc ctgtgagtac ggatttcacc
     4981 gaaaagaaga tttcctcgcg gaccgcctat cgcgggcgcc tgttgcaggt gaacgaggat
     5041 gaggtcaggt tgccggacgg cagccatgcc cagcgtgaat acatcctgca tcccggagcc
     5101 gccttgatcc tgccgatctt cgacgacggc tcggtgctgc tggagcgcca gttccgctat
     5161 ccggtcggat gccatttcta cgaactgccc gccggcaagc tcgagccaaa cgagccgccg
     5221 ctggaaaccg ccaggcgcga gcttcaggag gagacgggtt acgtggcggc ggagtggcgc
     5281 gagctcggcc gcctgcaccc ctgcgtcggg tactccaacg agaccatcga cttcttcctg
     5341 gcgcgcaagc ttgaattcaa gggcgcgcaa ctcgaccaag gcgagtttct ggagacgttg
     5401 cgattgccgc tcgccgaggg gcaggactgg gttcgccgcg gacgcatcag cgacacgaaa
     5461 accatcctcg gcctgttctg ggccgaaaag attctcaggg aaggctggta gaaaacccgg
     5521 ggggtcaggc gagcttggct gcctgccggt ccgcgctagc tgatgccggg aagggctgcg
     5581 ggtgaccgaa cacgaaaccc tggccgtact gcacgcccat ctcggtcagg attttcaata
     5641 cttcctggtt ctcgacccac tcggcaatca cttgtttgtc gagtccccgc gccacgtcgc
     5701 acaacgcgcg cacgaacacg cgattggctt cgtcgcttgc cagaccgcga atgaagttgc
     5761 cgtcgatctt caggtagtcc acttcgaagc gcttgaggaa atagaacgag ctgaaccccg
     5821 ccccgaaatc gtcgagcgca aagcggcagc ccagcttctt catctgcgag atgaactcct
     5881 gcgtgacgcc cacctccgcc atcgccgcgg tttccgtgat ctcgaacacc agttgttcgt
     5941 gatccaccgg tgcggccgcg agcatgccct ggaatcgctg cacccaatgc tggtcggaga
     6001 ggctcacgcg cgacaggttc acgaagtagc gcattttctg tccgcgcatc tgcggctgct
     6061 tcaggacttc caggaccttg gtcaccacgc gcaggtcgat ttcctgcgcc atgccgagcg
     6121 actcggccac ctcgatgaac tgtcccggca ggacgatgtt gccgctgcgg tcgacgatgc
     6181 gcaccagcac ttcgtggtgc accagcgtct gatccgcgag cctgaccacc ggctgcgagt
     6241 agagcagcac gcggtcttcg tccagcacct cgcgcagttc ccgcgcccag tgcacgcgcc
     6301 tgtgggtgct gcgcaggtcg tcgggattgt ggtcgtgcag tacgacacgg ttgcggccgt
     6361 tgtcctttgc ctggtacatg gcgatatcca cgttggcgag cagcgacgcc acgtcgttgc
     6421 cgtggaacgg ataggacacg acgccggccg aggccgtcag cgtcgacagg cagccctggt
     6481 cgcccaactg gaacttgtag tggcgcagcc cggtgagcgc gcgttcggcg acctgcgcgg
     6541 cctcggcgcg cagcgcatcc ggcaggtgca acgcgaattc gtcgccgccc agccggaaaa
     6601 cctgcgcgtt ctcgtctttc actacgtcct tgagcaggcc gccaatgccg atgatcaact
     6661 gatcgcccgc acggtggccg aaattgtcgt tgatgtactt gaagtgatcg aggtccagga
     6721 gcagcaccga acctttttcg ccgtctgcaa gcgcggcttc cagcactttc tgcagcgaaa
     6781 cccggttcgg caatccggtg agcgtatcgt gggtggcgaa gcgttcgatc tccagcgccg
     6841 ccttgtgttg ctcggtaacg ttgcgcgcag tgccgcgcag accgatgatc gcgccgttgt
     6901 cgtcatggga aacgcgcgcg ttgattccca cccagcggtc gttgccgtcg gcggatatga
     6961 gatgggtgag gtagttcttg acctccccgt tcttgcgcag cgcggacagg aagcggcggt
     7021 tggaaacgtg cgacagaccg gcttcgaagt cgaaaaagca acggccgatc agatccttct
     7081 gctgcaagcc gaatatgtcc acgcagccgt tgttgagata ggtgaaacgg cccagcgcat
     7141 cggtggtcca gatcaggtcg tgggaagtct ccaccagatc gcggtagcgc gactcgctct
     7201 ccagcagcag ttgtatgatg cggcgttttt ccttgagcgt cctgcgcgcg gccgccagtt
     7261 cgtccacgta cttctcggct tcgtgcttgt cgctgccggc taccagggca gaaccggcca
     7321 ggccgtcgat gatctcgcgc gcataacgcc ggtgacctcc gtcggtgcgg cgggccacca
     7381 gcacgccctg tgcgtcccag cgcctgagtt tctgcaccgg aatgcccagc tttgctgccg
     7441 cttcctgaat agaatactcg ggaagttcgt gcgccatcgc gcgcctcgca ctccattgtt
     7501 gccggttggt cgggccggca aaaccaacgt gccggcgcat cagtgcaggt gcttggcgac
     7561 gcccgcttcg tcccggcgcg caccggtggt agtgaagtcg ttctgccgcg aaaccagccg
     7621 ctgcatcagc ttgagatcgc ggtggctcag gttcagcgcc gtgtcgaccc acacctcgac
     7681 cacgcccatc aattcctcgt aatcgagctt atgcacgcgc ctgcgcgcgg ccgcgatgcc
     7741 agccatggca ttgcgactgc ggccgcggga gcggatgaag ctggcgattt ccgcttcacc
     7801 ccgcccctgg tccacgacca catccactac ccccattgcc agcatatcat cggcactgta
     7861 gatcttgcca ctggtgatca ggttttcggt cggacgcttg cccaccttcc gctccaggaa
     7921 ggaataggcc cccatccccg ggaacaggtt gaacatgatc tccggaaaac cgaagcgcgc
     7981 ctggcgctcc gcaaccagca cgtcggacga aagcgctgcc tcgaacccgc cacccaggca
     8041 ttcgccctgg attagcgaga tcgtggtgat cggcaactca tggccgatgt aattgcggta
     8101 caggacatcc acgcaggctt taccgtagcc gagcagaccg tcccggtcct gactgttgat
     8161 cagcctggtg aacaggccga ggtcgccgcc gagattgaac acgccgggca ccttggacgc
     8221 caggatcgcg aattcgacgg gtgcactctc ccctccacaa ttcacttcgc caccggtatg
     8281 ggtgatgaaa cggcagtagc ggtccaggtc acgcagcagg tcgggattga aattgggcct
     8341 cggagtcgga ttccagcgca accacatcgc atgttgctct ccgtcgaaaa acgcggaaag
     8401 ctgctccgag aaaaagtact cggtcgcttc cctggcaggc tcggcgccag tggctgccgg
     8461 gactttaagc atttcctgtg ggatattcat gtccattttt aattctcctg tgcgagtgga
     8521 tttaggatgt gaaaattggc cgccacagga cccccccaga gcggcttgcg atttgactct
     8581 agacgaagat acagcaaaaa acaaatctaa cactttgata gatttaattt ttttgctttg
     8641 ttgtcaataa tttggatgag cttgtcactc taagacgaca aaaaaatttg atgttttgta
     8701 tatcggcaga tggccggcca aagtatgtat gaacttcaag aataccgtgc aaacttcgga
     8761 ttttgttgac gttccaaaca ggacgcgcta gaaaggaaat ccataagaac gatggtttcg
     8821 cgcccgttcc caaatgcatt tccttctccg ctcaattgtg cggctgctgt tccgcgtcag
     8881 ggtcgtcggc gacttggcgc cattcggaca tgcggatcgg ctgctgctgg tctccaacca
     8941 ccattcgcgg cttgacgcgg tgttgctggc gttgttcctg ccgcgcgcgc cggtcatcgt
     9001 cgttccgccg gaggaaaccc gtagccggtt gatgcgctgg ttgctggggc acgtcgacca
     9061 cgaagtgcac gacctgaaca atcccatcag catgaaaccg ctgctgcggt tgctgcgcgg
     9121 cggacgcccc gtggtgctgt tccccgaggg gcgcatcgcg cgcaatgccg cggtgatgaa
     9181 ggtttacccg gtacctgcgt tgatcgcgct caagagcggt gccacggtgc tgccggtgca
     9241 cgtggaaggg gccggccagt ggttcagccg ctccggttcg acgtcagtca tgcgacggct
     9301 gctgccttcg atgacagtac gggtattcac acccacccgc atagacgggg gtgccgccgg
     9361 aagcgcgcga cggcgtcgcg ccttcgccac ccgcaggctc acctccatca tgcagaccat
     9421 ggcgctgcag tcgcatgcct gcaagccgct gttcgagtgc tttctcgatg cggccgcggt
     9481 gcacgggcgc ggcgcggtgc tgatggaaga tcagaacgag gatccgcaga cctacggcaa
     9541 gttgttgcgc atgagcctgg cgctgtcgcg gttgctgaag cgtttcacca gcgcgggcga
     9601 gaacgtcggc gtgctgctgc cgaacgtggt cccggcggtg ggcgtggtga tgggtctgtc
     9661 ggccaccggg cgcgtgccag ccattttcaa ctacgcggcg ggtccgctcg gcgtggaaag
     9721 cgcgcgggtt gcggccggtg tgcgcaccgt gatcacgtcc cgcaaattca tcgagcaggg
     9781 gcatctgcac tcgctgctgg atgcgcttgc cggctgcaac atcgtctatc tcgaggatct
     9841 gcgcagcggg ttcggctggc gcgacaagtt gtggctcatc ggtttggcat tgcgctttcc
     9901 gcgcctggtg attgccaggc agtccacgct cgatcccgcg gtggtgttgt tcacttccgg
     9961 ctcggaagac cggcccaaag gggtggtgct ctcgcaccat gccatcgtcg ccaacgtggc
    10021 gcagatgcgt gcggtgttcg acttctcgcc cgaggacaag atcctgaacc cgctgccgct
    10081 gtaccacgcc tacagtttta ccgccggcgt catgttgtcg ctgatcaccg gcacgcggct
    10141 gtacctcttc atttcgccgc tgcgctaccg cgccattccc gagattgcgt accgccgcga
    10201 ttgcaccgtc ctgttcggca ccagcacctt cctgtcgttc tacgcgcgct atgccaaccc
    10261 gatggacttc tgccgcttgc gctacgtgat ctccgggggc gaaaagctat cgccggaagt
    10321 ctcaagggtg tggatggaga aattcggatt gcgcatcctc gaaggctatg gttcgaccga
    10381 atgcgcgccg gtcatttctc tggccacgcc ctccgcgtac cgccgcaatg cggtgggccg
    10441 attcctgccc ggcatcgacc accagatcga gcccatggac ggcatcgaca agggcggagt
    10501 gctgcacatc cgcgggccga acctgatgct gggctactac cgctatgcca atcccggcgt
    10561 gatcgaggcg cccaagtcga gcgtgggcga aggctggtac gacaccggcg acgtggtgga
    10621 actggatcac gacggcatgc tcagcatctc cgggcgggtg aaacgcttcg ccaagatcgc
    10681 cggagaaatg gtgtcgctgg acatgatcga gcacgtagcg cgcgaagcat ctcccgggca
    10741 ccaccatgcc gcggtgctga cgatgcagga tttcgccggc gaatcgacgg tgctgttcac
    10801 taccgacgcg caactcaccc gcgcccaact gctggaggcg gcgaacgaac tggggacgca
    10861 cgatctttcg gtggctcgcc gcatcgtgca cctgcgggaa attccgttgc ttgcttccgg
    10921 caaggtggac tacgtcggac tcaagagcct catcgagggc gacgcggtca ggaaactgct
    10981 ggctgcaatg gccaggaacc cgctcgatat cgagtcccgt cccgccgcaa agtagtcgtt
    11041 gcctgcttcg aagcgctcac gcggcacacc gcaccaaccc cgtcggcgga aatcggcgga
    11101 gtcgatcacg tatcgactga agtttcagcg ggcgttcccg tttatatttt tgtcttagct
    11161 accggagatc gttgcttctc cccggtccaa ggtgcttgaa aacccggcac accgacccta
    11221 gttgcggctc tgtgatgaat cacggaaagg gcaatcccac catggcagaa gcagccggca
    11281 aacaaacgct gggcttccag actgaagtca agcaactgct caacctgatg atccactcct
    11341 tgtacagcaa caaggagatt ttcctgcgtg aactgatttc caacgcctcg gatgcctgcg
    11401 acaaactgcg cttcgaggcg ctgaccgatc cggggctgtt cgaggccgat cccgatctgc
    11461 gcatacgggt ctggtacgac aaggcggctc gcgccctcac catttccgac aacggcatcg
    11521 gcatgtcccg ccaggaggtc atcgacaaca tcggcacgat cgccaagtcc ggcacccgcg
    11581 agttcttcga tcgcctcacc ggcgatcagg ccggggacgc ccacctgatc ggccagttcg
    11641 gcgtgggttt ctattcctct ttcatcgttg cggacaaggt cacgctggtt acgcgccgcg
    11701 ccggcctggg caaggaacac ggcgtgcgct gggaatcgtc cggcgagggc gactacacca
    11761 tcgagaccgt ggaacagcag ggtcgcggca ccgacgtgac gctgcacctg cggatgggcg
    11821 aggacgaacc gctggacggc ttccgtttgc gttccatcat gcgcaagtat tccgaccaca
    11881 tcacgctgcc catcctgatg aaaaaagagg agtggagcca ggagaagggc cagaacgtcg
    11941 tcaaggacga agacgaaaag gtcaaccagg cctccgcgct gtgggcgcgc cccaagtccg
    12001 agatcagcga agagcaatac caggagttct acaagcacgt tgcccatgac ttcgagccgc
    12061 cgctggcctg ggtgcacgca agagtcgagg gcaagtccga gtacacccag ttgctctacc
    12121 tgccggcgcg cgcacccttc gatctgtggg accgcgagca ccgccacggc atcaagctct
    12181 acgtgcggcg cgtgttcatc atggacgatg ccgagcaact gatcccggcc tacctgcggt
    12241 tcgtccgcgg ggtggtcgat tccaacgacc taccgctcaa cgtctcgcgc gaaatcctgc
    12301 aggaatcgaa ggacgtcgag gtgatccgca agggctgcgt caacaaggtg ctcggtctgc
    12361 tcgaagatct cgcggagaac cagaaggaga agtacgcgcg tttctggaag gaattcggcc
    12421 acgtgttcaa ggaggggacc ggcgaagacg gggcgaacag ggaacgcctc gccaggctgc
    12481 tgcgctttgc ctcgactcac ggcgatagcg acgggcaaac catttcactc accgactacc
    12541 tgtcgcggat gaaggaaggc caggacaaga tctactacat caccgccgac ggcttggctg
    12601 cggcgaagaa cagcccgcat ctggaaatct tccgcaagaa gggcatcgaa gtgctgctgc
    12661 tttccgaccg ggtggacgaa tgggtggcgt ccaatctcac cgaattcgaa ggcaagccgc
    12721 tgcagtcggt ggccaagggc gatctcgatc tgggtaagct cgccgacgag gagaagaagg
    12781 aacaggaaaa ggaggccggc gaatacaagg acctggtcga gaaaataagg aaagcgctgg
    12841 atgcccgggt gaaggaggta cgactgacgt tccggctgac atcgtcgccg gcctgcctgg
    12901 tgtcggacca gaacggcatg agcgccaact tggagcgcat gctcaaggcc gccggccaga
    12961 ag
//
LOCUS       DASQOP010000089        15142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1645768, whole genome shotgun sequence.
ACCESSION   DASQOP010000089 DASQOP010000000
VERSION     DASQOP010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 15142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15142
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1645768"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VE030_03510"
     CDS             complement(<1..447)
                     /locus_tag="VE030_03510"
                     /inference="COORDINATES: protein motif:HMM:NF025881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HZF22517.1"
                     /translation="MPYRTVKALSPVDAAYMAGLIDGEGTVTLSRRHRNDQRQLVVSI
                     ANTERRLLEWTLNTVGAGKITGKRISSPLHSPSFTYSIANRQALQLLEQIALYLRSYK
                     ATRTQLILQNYINLTPRNGRYTEEIRAERDRFLTSVLEIKAQEPADS"
     gene            complement(842..1201)
                     /gene="ssrA"
                     /locus_tag="VE030_03515"
     tmRNA           complement(842..1201)
                     /gene="ssrA"
                     /locus_tag="VE030_03515"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1256..1960)
                     /gene="gph"
                     /locus_tag="VE030_03520"
     CDS             complement(1256..1960)
                     /gene="gph"
                     /locus_tag="VE030_03520"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114415.1"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009853 - photorespiration [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HZF22518.1"
                     /translation="MVVPLTLPGRGVLFDLDGTLADTAPDLGFALNQQRLARGMPELP
                     VAVVRSQASSGARGLLKIGFGIEPGQSSYEAMRDEFLDIYEENLARSSRLFPGVSALL
                     EQIERRGLRWGIVTNKAERFTFPLLRALSLMERAGCVICGDTTPNPKPHPAPLLAAAE
                     KLRVPPGQCIYVGDDERDVQAGHAAGMPVVVARYGYLGNGTPPEHWGADGFVDAPADL
                     LGVLFDGTRGRSDRMA"
     gene            complement(1977..2561)
                     /locus_tag="VE030_03525"
     CDS             complement(1977..2561)
                     /locus_tag="VE030_03525"
                     /inference="COORDINATES: protein motif:HMM:NF015858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajG family lipoprotein"
                     /protein_id="HZF22519.1"
                     /translation="MKTRLWFVLAVFGLVAALQGCSLKPQNLRIDPPVKVNESAVGSG
                     RTIGLGVSDARAAKKLGEIGDPDTKMVDVSVEEDPSAAVYERLQEALTKLGFSVVPYS
                     NSMERTLQVEIRNLELKSVKTPFTFETELRAAVGARVDNGPEYYDRQFNVRTRKDGAA
                     PPFEKDSTLLVNTAVSQALEDMLSDEQLLELLAK"
     gene            complement(2646..3341)
                     /gene="ubiG"
                     /locus_tag="VE030_03530"
     CDS             complement(2646..3341)
                     /gene="ubiG"
                     /locus_tag="VE030_03530"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010099676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HZF22520.1"
                     /translation="MINVDPLELEKFSQLAHRWWDPNSEFKPLHDINPLRLDYIDGIA
                     NLRGTKVLDVGCGGGLLSEAMAARGAKVTGIDMGDKPLKVAQLHLLESKLDVEYRKVE
                     VEQLAREQPHRYDVVTCMEMLEHVPNPASAVKACADLLAPGGWCFFSTINRNPKAYLF
                     AIVGAEYILKLLPRGTHDYSRFITPAELGRFCRGAGLSPQRVTGMTYNPFTRTYALGT
                     DSSVNYILSAQAA"
     gene            complement(3360..4697)
                     /locus_tag="VE030_03535"
     CDS             complement(3360..4697)
                     /locus_tag="VE030_03535"
                     /inference="COORDINATES: protein motif:HMM:NF006549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HZF22521.1"
                     /translation="MQQMNTLFSADTVIEARWIIPIEPHGAVLNDHSVAIAGDRITAV
                     LPRAEMRRRCKAGRTIDLSTHALIPGLVNLHTHAAMALMRGLADDLPLMDWLEKHIWP
                     AEARHVSAQFVYDGTVLACAEMLRGGVTCFNDMYFFPEMAGRAALDAGIRAVLGIVLV
                     EFPTAYASDAQDYLRKGLAMRDELKSEPLLSFCMAPHAPYTVSDRSFEQILTLSEQLQ
                     IPINTHLHETRGEIRESEGKFGMRPLARLEKLGLLGPSFIAIHAVHLNPAEIALLARL
                     GASVAHCPSSNLKLASGIAPVGAMLSAGMNVGLGTDGAASNNRLDVLSEMRLAALLAK
                     AASDNAETLPAHVALRMATLNAARALGLEQKVGSVETGKCADLCAVNLASIELSPCYD
                     PASQLVYAAGRENVSHVWVGGKLLVEQGRLLHVDEAAVARKAREWQSRIAASN"
     gene            complement(4762..5997)
                     /locus_tag="VE030_03540"
     CDS             complement(4762..5997)
                     /locus_tag="VE030_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZF22522.1"
                     /translation="MRPKTNRLLMLLISAMLAGCASQTERSSSGGGAEQAQAPAPAPA
                     PAPAYEPQSENVQFSDRPLEETPSPRPAPVAKAPEQPLPDFPITKYEVEPEEEVADLG
                     TQPDESGTTQYPEDDLAAKDSVVSGPTEAADEEEEEEVGDLGTQPDESGTMQYPEEDL
                     AAKDSAVGEPTAGADEPEEEVGDLGTQPDESGTMQHPEEDLAAKDSTVGEPAAVANEP
                     EEEVADLGTQPDESGTMSYPEQQIASENVVGEPKQYADEPAAAPEPAVAAAPEPTPAP
                     VPVAKAKAPVRVSFEAEPLFSFDKSVVRSDQRGKLDEFVASLKGTKYDSIEVIGHADR
                     IGSEDYNLKLSQRRANAVKTYLVRQGIPAGKLNAVGRGKSEPVTGDTCNGQRGKALIA
                     CLEPDRRVDVSVTATKPAN"
     gene            6343..9006
                     /gene="gyrA"
                     /locus_tag="VE030_03545"
     CDS             6343..9006
                     /gene="gyrA"
                     /locus_tag="VE030_03545"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013206616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HZF22523.1"
                     /translation="MDQFAKETLPISLEEEMRRSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRILFAMHEANNVWNRPYVKCARVVGDVLGKYHPHGDSATYEALVRMAQDFSMRYTL
                     VDGQGNFGSVDGDSAAAYRYTECRLEKIASELLADIDKETVDFVPNYDGKEQEPSVLP
                     TRIPNLLINGSAGIAVGMATNVPPHSLHEIVDACLAILERPEIGVDELMEIIPGPDFP
                     TRGIIYGTSGVKEGYRTGRGRVVIRARTHMEELEKGNRQAIVIDELPYQVNKSALLIK
                     IGELVRDKRLDGLSEIRDESDKSGMRVVLELKRGEVPEVILNNLYKETQMQDTFWMNM
                     VALVDGQPRLLNLKEILDAFLRHRREVVTRRSVFELRKARERGHILEGLAVALSNVDE
                     IIALIKAAPAPAEAKRELMSRMWRSQLVENMLARAEVSASRPEGLGRDYGLQSNGYRL
                     SEVQAQRILEMQLQRLTGLEQEKIVNEYREVMDNIADLLDILAKPERITRIIAEELAE
                     TRSQFGDERRSEIVMDAQDLSMEDLIAPEDVVVTLSHTGYVKAQPLADYRAQKRGGRG
                     KQATATKEDDFIDNLFIANTHDYVLCFSNRGRLYWIKVYNVPQGGRASRGKPIVNLLP
                     LVDGEKITAILPVKEFDEHKYVFMATSNGTVKKTPLSDFSRPRPSGIIAVDLEEGDYL
                     IGVALTEGRQDIMLFSDAGKAVRFDEQDVRPMGRLARGVRGMRLSGGQKVISLLVADN
                     ETVSVLTATENGYGKRTPITEYTRHGRGTQGMIAIQTSARNGNVVAATLVEESDEIML
                     ITTGGVLIRTRVKEIREMSRSTQGVTLINLGPEEKLAGLQKIVDKDDDVGPDQDLAPE
                     TEAAADPDADPDRIESADGPLEQDPGGDETS"
     gene            9050..10132
                     /gene="serC"
                     /locus_tag="VE030_03550"
     CDS             9050..10132
                     /gene="serC"
                     /locus_tag="VE030_03550"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HZF22524.1"
                     /translation="MGRVFNFSPGPAVLPQLVLEQARDELLDWHGTGMSVMEMSHRSK
                     EFIAIAEAAEADLRALLAIPANYRVLFLQGGATAQFAMVPMNLLRGKKSADYIHTGEW
                     AKKAIAEAGKFCSVNVAASSADRNFSYVPRQAAWTLGPESGYVHITSNETIGGIEFHW
                     LPDTGSVPLVADMSSHILARRLDVSRFGLIYAGAQKNIGPAGLTIVIVREDLIGQALP
                     GTPAMLDYKIHADNGSMYNTPPTYALYIAGLVFQWLKKMGGLGEMEKVNLLKAGLIYQ
                     YLDGSRFFTSPVAEEDRSIMNIPFRLKDDALDEEFLKQARARGLTQLKGHRSVGGMRA
                     SIYNAMPVEGVRALVQFMREFEARHG"
     gene            10125..11312
                     /locus_tag="VE030_03555"
     CDS             10125..11312
                     /locus_tag="VE030_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HZF22525.1"
                     /translation="MADPVRILTLNSISQVGLKRLPAERYTVGTHVQSPDAILVRSQD
                     MHAMDIAQSVKAIGRAGAGTNNIPVAAMNERGVPVFNAPGANANAVKELVIAGMLMAA
                     RNLVAAIRFVDALSVTDEEINKKVEEGKKHYTGVELPHHVLGIIGLGRIGSLVADAAI
                     KLGMNVHGYDPEITVDAAWSLPSQVKKAHSIEEVMRNSDFVTIHVPLLEETRNLIEAK
                     RIGLMKHGAVLLNFSRESIVDNAAVLQALDARRLKAYVCDFPSGSFRGHSGVVALPHL
                     GASTREAEDNCAVMVVEQVRDYLENGNVRNAVNFPDVTVVRESPFRLGIANANVPNMV
                     GQISTAVAQAGLNIHNMLNKSKKDMAYTLVDVDSPVPSKVIDKIAAIEGVLAVRYLPS
                     EAS"
     gene            11312..12379
                     /gene="pheA"
                     /locus_tag="VE030_03560"
     CDS             11312..12379
                     /gene="pheA"
                     /locus_tag="VE030_03560"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HZF22526.1"
                     /translation="MSDELKQLRSRIDALDQEILRLISERAAAAHAIGKLKGDGPVYR
                     PEREAQVLRQLADRNPGPLPDQAVMYLFTEIISACRALEDAFSVACLGPKGTFSEEAV
                     IKHFGSQAPATLCGSIDEVFRAVEAGSVGYGVVPVENSTEGAVGRTLDNLLSTPLKIC
                     GEVVLPVHQNLLSRAASMAAVKRVYSHAQSLAQCSRWLSQNCPRAELVPVASNAEAAR
                     MAATEAGAAAIAGHSAAKHYGLPVLAANIEDEPNNTTRFAVISRQEAPPSGNDKTSLV
                     MSTRNIPGAIHELLTPLARCGVSMTRLESRPSRTGLWEYVFFVDLEGHQTEDKVARAL
                     DEMRGIAAFLKILGSFPVAAQ"
     gene            12376..13512
                     /gene="hisC"
                     /locus_tag="VE030_03565"
     CDS             12376..13512
                     /gene="hisC"
                     /locus_tag="VE030_03565"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HZF22527.1"
                     /translation="MNTLSSLQDIVDVCERSPSYVRAIAPYQPGKPISELAREIGLEE
                     GSIIKLASNENPLGASPKALAAMRAALDEVARYPDGNGFELRTALAKMYGVAAARIVL
                     GNGSNDVLEMAAHAFLEPGASAVYSQHAFAVYPLATQATGAAGIEVPARDFGHDLDAM
                     GRAVRGDTRVVFVANPNNPTGTWIAADRLHAFLRKLPAHVLLVLDEAYYEYLDPGLRS
                     DSLGWLEQFPNLVVSRTFSKAYGLAGLRVGFAFAHPQVAGLMNRVRQPFNVSSIAQAA
                     AVAALADQEFVHKSRALNAQGMLALTTGFKRLGLSWIPSYANFVSFKVPNTAEVFQRL
                     LRRGVIVRPIAGYGMPEHLRVTVGTGAENARFLAELEAVLTDLR"
     gene            13565..14419
                     /locus_tag="VE030_03570"
     CDS             13565..14419
                     /locus_tag="VE030_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HZF22528.1"
                     /translation="MVVIGVGLIGGSVAAALRRAGKVRRVIGVGRGRANIARALELGV
                     IDDAVEDIAAAVKGADAVLIAVPVQQNPHVLGAFADALAPGTLVTDAGSTKQDFVAAV
                     RRIAPRHLASVVPGHPIAGAELTGVDAASAALFDGRNVVLTPLEENQAAVVDRVEAMW
                     QACGARVSRMTPERHDRVFSAVSHLPHMLAYTLVHMIATRADAEALFGFAASGFRDFT
                     RIAGSSAEMWRDIALANRDALLADIEAYQQELAELAQCLRQADGAEIERIFEAARCAR
                     NAWIRNGS"
     gene            14423..>15142
                     /locus_tag="VE030_03575"
     CDS             14423..>15142
                     /locus_tag="VE030_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003278016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HZF22529.1"
                     /translation="MAAYPEYLELGPFDRATGRLRLPGSKSISNRVLLLAALGHGITG
                     VHDLLSADDVNRMLEALEKLGVKLQKGKEPGHLSINGCGGTFPVKSAELFLGNAGTAL
                     RPLTAALALCGGHYRLSGVPRMHERPIGDLVDALRQVGANVRYLGKEGFPPLEIRAGN
                     IDDAGKVRVSGEVSSQFLTALLMALPLTGKQTTIEVVGDLISKPYVEITLNLMARFGV
                     QVRRAGWSSFTIPADSRFHSPG"
BASE COUNT         2881 a         4626 c         4753 g         2882 t
ORIGIN      
        1 tgagtcggca ggttcctggg ctttgatctc cagtactgac gtgagaaaac gatcccgctc
       61 tgcacgaatc tcttcggtat accgcccgtt ccggggggtg aggttgatgt agttctggag
      121 gatgagttgt gtgcgcgtgg ctttgtaaga cctcaggtac agcgcgattt gttcgagtag
      181 ttgtagggcc tgacgatttg caatcgaata cgtgaagctg ggcgagtgta gtggactgga
      241 gattcgcttt ccagtgatct tcccggcacc cacggtattg agcgtccatt caagaagcct
      301 gcgttcggta ttggcgatgc tcacaaccaa ttgacgttga tcgttgcgat ggcgtcggct
      361 gagggtaacc gtaccttctc cgtcgatcag tccggccatg taagccgcgt ccacagggga
      421 aagggcttta acggttcgat agggcatgac ttaactcccc tattggcgaa aaggaaatga
      481 taaagaaaaa gcccccggct tgcgaccgga ggctttctac caaattggtg gaggcggcgg
      541 gacagttgtt ccactccttt cggagcaggc tggactattc cttcatcccg gttgcccggg
      601 atgcggggcg tgttatccga tattccggat cctagtgtcc gcccgccggc agagccggtc
      661 ggcgagtcta tgagtctcta gagggcaggc ctgaagttgg ccttcttcct acccctcggc
      721 gttgccactc cgtccttggc aagacggttt gaggtttcac cgatgtgagc cccgttccga
      781 ccggcattgc ttcgccggcc gcgacggatg cttcctgggg catccgccgt cactttaccc
      841 ttgcgggtaa aggcaacctt cgatcgaacc cgcgtccgca agccctctac aggcagtcct
      901 acatacttag tcaggtcatt tggtctaacc acttccacgc cggccgacag gctgggaagc
      961 ggcgagtcac cttggatttg atgctgcaat aagtgaccca cggcagcacg atctcacgta
     1021 tatggctccg ctgctggttg cccagcccgg cccgtgagag aaccggtgcg gagtccagcc
     1081 gggtttaggc ggctagagcg taacgttcgt cgttggcgac tatacgtttt ccagctgtat
     1141 taacaaggta actgggcctt ggtatgcact gcactgcttc gcgacccacg tcgagaccag
     1201 gtcgccccca tgtaaaactt agatggtgcc gaccctgaaa tgtttcaacc agagtctaag
     1261 ccattctatc agatcggccg cgtgtcccgt cgaatagaac gcccagcagg tctgcggggg
     1321 catcgacgaa accatccgcg ccccaatgtt ccggcggtgt tccattgccg agatagccgt
     1381 agcgcgccac cacgacaggc attcccgctg catgtccggc ttgcacatcg cgttcgtcgt
     1441 cgccgacata aatgcattgc ccggggggaa cacggagctt ttccgccgcc gcaagcaaag
     1501 gcgccggatg cggcttcggg ttcggcgtcg tgtcaccgca tatgacgcat ccggcacgtt
     1561 ccatcaggct cagcgcacgc agcaggggga aagtgaaccg ttccgccttg ttggtgacga
     1621 taccccagcg cagtccgcgt cgttcgatct gctccagaag cgcggagacg cccgggaaca
     1681 gccggctgct gcgggcaaga ttctcttcgt aaatgtccag gaattcgtcg cgcatggcct
     1741 cgtagctgct ttggcccggt tcgatgccaa agccgatttt caacagtcca cgcgcgccgg
     1801 aagaggcctg cgacctgacc accgcgaccg gcaactcggg catgccgcgg gcaagacgct
     1861 gttgattcag cgcaaagccc aggtcgggag cggtatccgc cagcgtgccg tcgaggtcga
     1921 agagcacgcc acggccgggc agggtcagtg gtaccaccat caagttcttt ggatttctat
     1981 ttagcgagca gttccagcaa ctgctcatcg gacagcatgt cctccagcgc ctgtgaaacc
     2041 gcggtgttca ccagcaaggt gctgtccttt tcgaagggtg gcgcagcgcc gtccttgcgg
     2101 gtccggacgt tgaactggcg gtcgtagtat tcggggccat tgtccacgcg ggcgccgact
     2161 gccgcccgca actcggtttc gaaggtaaac ggtgtcttga ccgacttcag ttcgagattg
     2221 cggatctcca cctgcaacgt gcgttccatc gagttgctgt acgggaccac cgagaaaccc
     2281 agtttggtca gggcttcctg caggcgttca tacaccgccg cactcgggtc ctcttccacc
     2341 gagacatcca ccatcttggt gtccgggtca ccgatttcgc cgagcttctt ggctgcacgc
     2401 gcgtccgaaa cgccgagtcc gatggtcctg ccgctgccga cggcggattc attcactttc
     2461 accggtggat caatgcgcag attctgcggt ttcagcgaac agccctgaag cgcggccacc
     2521 aagccgaaca ccgccagcac gaaccagagt cttgtcttca tgttgtgatc ctccttggat
     2581 atcttgccaa cacttaaatc gcggcgaaca accaacaatg cagcaggagc gtaatcgtac
     2641 gtaggtcagg cggcctgcgc gcttaggatg taattcaccg aactgtccgt gccaagcgca
     2701 taagtcctgg tgaacgggtt gtaggtcatc ccggtcacgc gttgtggtga cagtccggcg
     2761 ccgcggcaaa aacgccccaa ttccgccggg gtaatgaacc tggaataatc gtgcgtaccg
     2821 cgcggcagca atttcagaat gtactccgcg ccgactatcg cgaacaggta agccttcggg
     2881 ttgcggttga tggtggaaaa aaaacaccag ccgccgggcg caagcaagtc ggcacaggcc
     2941 ttgacggcgc tcgccgggtt gggcacgtgc tcaagcatct ccatgcaagt gacgacgtca
     3001 tagcgatgcg gctgttccct ggcgagttgc tcgacctcca ccttgcggta ttcgacgtcg
     3061 agcttgctct ccaacaggtg cagttgcgcc actttcaagg gcttgtcgcc catgtctatg
     3121 ccggtcactt ttgcgccgcg ggccgccatc gcctccgaca acagacctcc gccgcagcct
     3181 acatcgagaa ctttcgttcc ccgaagattt gcgatgccgt cgatgtaatc cagccgcagc
     3241 ggattgatgt cgtgcagcgg cttgaactcg ctgttcggat cccaccagcg gtgcgcaagc
     3301 tggctgaatt tttccagttc gagcggatcg acgttgatca tctcaggctg atgccccgct
     3361 tagtttgacg ccgcaatacg cgactgccat tcccgggcct tgcgtgcaac ggccgcttcg
     3421 tcaacgtgca acaaccggcc ctgctcgacc agcagcttgc cgccgaccca gacatggctg
     3481 acgttctccc gtccggcagc atagaccaac tgcgaagccg ggtcatagca gggagacagc
     3541 tcgatcgacg ccaagttcac ggcgcacaga tccgcgcact tgcccgtctc gaccgacccg
     3601 actttctgtt ccaggcccaa cgctctggcc gcgtttagcg tcgccatccg cagcgcgacg
     3661 tgcgcgggta aggtttccgc gttgtcactc gccgccttgg cgagcaacgc tgcaagccgc
     3721 atttcggaaa gcacgtcgag ccggttgttg ctcgccgcgc cgtcggtgcc aaggccgacg
     3781 ttcattcccg ccgacagcat ggcacccacc ggcgcgatcc cgctggcaag cttgaggttc
     3841 gacgaggggc aatgcgctac cgaggcgccg aggcgtgcca gcagcgcgat ctcggctgga
     3901 ttgaggtgga ccgcatgaat ggcgatgaag ctcggtccga gcaggcccaa cttttccagc
     3961 cgggcaagcg gccgcattcc gaacttgcct tcgctttccc ggatttcgcc gcgcgtttcg
     4021 tgcaggtgcg tgttgatagg aatctgcagc tgttcggaaa gcgtgaggat ttgctcgaaa
     4081 ctcctgtcgc tgacggtgta gggggcatgc ggtgccatgc agaaggacaa caagggctcg
     4141 ctcttgagtt cgtcgcgcat cgccagcccc ttgcgcaggt aatcctgtgc atcgctcgcg
     4201 tatgccgtcg gaaattcgac cagcacgatc cccagaacgg cgcggattcc ggcatccaac
     4261 gcggcacgtc cggccatttc cgggaaaaaa tacatgtcgt tgaaacaggt gacaccgccg
     4321 cgcagcattt ctgcacaggc aagcacggtg ccgtcgtaca cgaactgcgc cgagacgtgc
     4381 ctggcctcgg cgggccagat gtgtttttcc agccaatcca tcagtggcag atcatcggca
     4441 agcccgcgca tcagcgccat cgccgcgtga gtatgcaagt tcaccagtcc cgggatcagt
     4501 gcatgggtgg aaagatcaat agttcggccg gccttgcacc gccgccgcat ttccgcgcgc
     4561 ggcaataccg cggtgatgcg atcgccggcg attgccacgc tgtggtcgtt caaaacggct
     4621 ccgtgcggtt cgatgggaat gatccagcgc gcctcgatca cggtgtcggc agaaaagagc
     4681 gtgttcatct gctgcatgga tcttccaaaa aaaagccctg cattcgcagg gctttcattc
     4741 gcttgtaggc aatccgcagg gtcaattggc aggcttggtt gccgtgacgc tgacatccac
     4801 ccggcggtcc ggctccaggc aggcaatcaa tgctttacca cgttggccat tgcaagtgtc
     4861 tccggtcacc ggctccgatt tgccgcgccc cacggcattg agcttgccgg cgggaatgcc
     4921 ctggcgcacc aggtaggtct tcaccgcatt ggcgcggcgt tgtgacagtt tcagattgta
     4981 gtcctcggat ccgatgcggt cggcgtgccc gatgacctcg atcgagtcgt atttcgttcc
     5041 cttcaggctg gcaacgaatt cgtcgagctt gccgcgctgg tcgctgcgaa cgacggactt
     5101 gtcgaagctg aacagcggtt ctgcttcgaa gctgactctc accggcgcct tggccttggc
     5161 cacaggcaca ggtgcaggcg taggctcagg cgcggccgca accgcaggct caggcgcagc
     5221 cgcaggctcg tcggcgtact gtttcggttc gccgaccaca ttctcggagg caatttgctg
     5281 ctcggggtaa ctcatggtgc ccgactcgtc aggctgggtg cccagatcgg cgacttcctc
     5341 ttcgggttcg ttggctaccg ctgccggttc gccgaccgtg ctgtcctttg ctgcgaggtc
     5401 ttcttccggg tgctgcatcg tgcccgattc gtcgggctgc gtgcccagat cgccgacttc
     5461 ctcttcaggt tcgtccgccc ccgccgtcgg ttcgccgacc gcgctgtcct tcgctgcgag
     5521 gtcttcttcc ggatattgca tcgtgcctga ttcgtccggt tgcgtgccca gatcgccgac
     5581 ttcttcttcc tcttcttcgt cggcagcctc ggtcggcccg ctcaccacgc tgtccttggc
     5641 ggcaaggtcg tcttcggggt actgcgtggt gcccgactca tccggctgcg tgccgaggtc
     5701 tgcgacctcc tcttcgggct cgacctcgta cttggttatc ggaaaatcgg gaaggggttg
     5761 ctcgggtgct tttgcaaccg gagccggtcg cggagacggt gtttcttcga gcgggcgatc
     5821 cgagaactgc acgttttcgc tctgtggttc atacgcgggt gccggggcag gagccggtgc
     5881 cggggcctgc gcctgttcag caccgccacc agagctgctg cgttcggtct ggctggcaca
     5941 gcccgcaagc attgcgctga tcaacaacat caacagccga ttggtcttgg gtcgcatttc
     6001 gggttctcct gatcatgctg gtcaaggact cttgcattat tgcccattcc cgggccgctt
     6061 aacaacgctt aacacggatc gcatcgcaac ggtcgcagta gcgcagccga ggcgcatgaa
     6121 tcatggccgt attgccgcag ggccgaacca cccgcaaagt caggcaaaaa gccgcgccgg
     6181 acaatgtctc ggggataagc gccatgataa aatgcacggc ttgcgctgga acgaatcaag
     6241 gggcttccca gcgagccatc ccctccaaaa gtcctggcaa gtctatcggg tcgggcggga
     6301 cagaaccgtg actgtttgca acaagaaaac tgaccattcc tgatggatca attcgccaaa
     6361 gaaaccctgc ccatcagcct ggaagaggag atgcggcgct cctacctcga ctacgcgatg
     6421 agtgtgatcg tgggtcgggc gctgcccgac gtgagagacg gcctcaagcc ggtgcaccgc
     6481 cgcatccttt ttgcgatgca cgaagcgaac aacgtgtgga accggcctta cgtgaaatgc
     6541 gcgcgcgtgg tcggcgatgt actcggcaag taccatccgc acggcgatag cgccacctac
     6601 gaagccctgg tgcgcatggc gcaggacttc tcgatgcgct acaccctggt cgacggacag
     6661 ggcaatttcg gttcggtgga cggtgattcg gcggcggcct accgctatac cgaatgccgt
     6721 ctggagaaaa tcgcctcgga gttgctggcc gatatcgaca aggaaaccgt cgatttcgtc
     6781 ccgaactacg acggcaagga gcaggagccc agtgtcctgc ccacccggat acccaatctg
     6841 ctcatcaacg gctctgccgg tatcgcggtg gggatggcca ccaacgttcc accgcacagc
     6901 ctgcacgaaa tcgtcgatgc ctgcctggca atcctggagc ggcctgaaat cggcgtcgac
     6961 gaactgatgg aaatcatccc gggaccggac tttccgaccc gcggaatcat ctacggcacc
     7021 tcgggagtca aagagggtta tcgcactggc cgcggccggg tggtaatccg cgcccgcacc
     7081 cacatggagg agctggaaaa gggcaaccgc caggccatcg tcatcgacga actgccttac
     7141 caggtgaaca agtcggcact tctcatcaag atcggcgagt tggtgcgcga caagcgcctc
     7201 gatggcctct cggaaatccg cgacgaatcc gacaagtccg gcatgcgcgt ggtgctggag
     7261 ctcaagcgcg gcgaagtccc cgaagtcatc ctgaacaacc tgtacaaaga aacacaaatg
     7321 caggacacgt tctggatgaa catggtggcg ttggtggacg gtcagccccg cctgctcaac
     7381 ctgaaggaga ttctggacgc cttcctgcgc caccggcgcg aagtggttac ccgccgctcg
     7441 gtgttcgaat tgcgcaaggc gcgcgaacgc ggccacatcc tggaaggact ggcggtggcg
     7501 ctttccaacg tcgacgagat catcgcgctg atcaaggccg cgcccgctcc agccgaagcc
     7561 aagcgcgaat tgatgtcgcg catgtggcgt tcgcaactgg tggagaacat gctggcacgc
     7621 gccgaggttt ccgcttcgcg ccccgagggc ctgggacgcg actatggatt gcagtccaac
     7681 ggctaccggc tttccgaggt gcaggcgcag cgcatcctgg aaatgcagtt gcagcgcctg
     7741 accggactgg agcaagagaa aatcgtcaac gagtacaggg aggtcatgga caacattgcc
     7801 gacttgctcg acatccttgc caagccggag cgcatcacca ggatcatcgc cgaggagctt
     7861 gccgagaccc gcagccagtt cggcgatgaa cgccgcagcg aaatcgtgat ggacgcccag
     7921 gacctcagca tggaggacct gattgcgccc gaagacgtgg tggtgacgct gtctcacacc
     7981 ggctacgtga aggcccagcc gctcgccgac taccgggcgc agaagcgcgg tggtcgcggc
     8041 aagcaggcca ccgcgaccaa ggaagacgat ttcatcgaca acctgttcat cgccaatacc
     8101 cacgactacg tgctgtgctt ctccaatcgc gggcggctgt actggatcaa ggtctacaac
     8161 gtgccgcagg gcgggcgcgc cagccgcgga aaaccgatcg tcaacctgct gccgctggtg
     8221 gacggcgaga agatcaccgc gatcctgccg gtcaaggagt tcgacgagca caagtacgtg
     8281 ttcatggcca cgtccaacgg cacggtcaag aaaacgccgc tttcggattt ttcgcgcccg
     8341 cgcccgtccg ggatcatcgc ggtggacctg gaagaggggg actacctgat cggggtggcg
     8401 ctcaccgagg ggcggcagga catcatgctg ttctccgacg ccggcaaggc ggtgcgtttc
     8461 gacgagcagg acgtgcggcc gatggggcgc ttggcgcgcg gtgtgcgcgg catgaggctc
     8521 tccggcggcc agaaagtcat ctcgctgctg gtcgcggaca acgaaaccgt gtcggtgctc
     8581 acggccaccg agaacggcta tggcaagcgt accccgatca ccgagtacac ccgccacggc
     8641 cgcggcaccc aggggatgat tgcgatccag accagcgccc gcaacggcaa tgtggtcgcg
     8701 gccacgctgg tcgaggaaag cgacgaaatc atgctcatta ccaccggcgg cgtgctgatc
     8761 cgtacccggg tcaaggaaat ccgcgaaatg agccgttcca cccagggcgt gacgctgatc
     8821 aatctcggtc cggaagagaa gctcgccggc ctgcagaaaa tcgtggacaa ggacgacgac
     8881 gtgggcccgg atcaggactt ggctccagaa actgaagctg ccgccgatcc cgacgctgat
     8941 ccggaccgga tcgagtccgc cgacgggccg ctggaacagg atcccggtgg ggacgaaacg
     9001 agttgaagca gtctgcagcg aattccaatc catagaacga aagaaaaaag tgggaagagt
     9061 tttcaacttc agtccggggc cggcggtcct gccgcaactg gtcctggagc aggcgcgcga
     9121 cgaattgctc gactggcatg gcaccggcat gtcggtcatg gaaatgagtc accgcagcaa
     9181 ggaattcatc gccatcgctg aagcggcgga agccgacctg cgcgcgctgc tcgccattcc
     9241 cgcaaattac agagttctgt tcctgcaggg cggggcaacc gcgcagttcg ccatggtgcc
     9301 gatgaacctg ttgcgcggaa aaaagtcagc cgactacatt cacaccggcg aatgggccaa
     9361 gaaggcgatt gccgaggccg ggaaattctg cagtgtcaac gtggcggcga gttcggcgga
     9421 cagaaatttc agctacgtgc cgagacaggc tgcatggacg ctcggcccgg aatcgggata
     9481 cgtgcatatc acatccaacg aaaccatcgg cggcatcgag tttcactggc tgcccgatac
     9541 cggtagcgtg ccgctggttg cagacatgtc gtcgcacatc ctggcacggc ggctggatgt
     9601 gtcgaggttc ggcctgatct atgcgggcgc gcagaaaaac atcggccctg ccggactgac
     9661 catcgtgatc gtgcgcgagg acctgatcgg ccaggcgttg ccgggcaccc cggcgatgct
     9721 cgactacaag atccacgccg acaacggctc gatgtacaac acgccgccca cgtatgcgct
     9781 ctatatcgcc gggctggtgt tccagtggtt gaagaaaatg ggcgggctgg gcgagatgga
     9841 aaaggtcaac cttctcaagg ctggcttgat ttaccagtat ctcgatggca gccggttctt
     9901 caccagtccg gtggcggagg aagatcgctc gatcatgaac attccattcc ggctcaagga
     9961 cgacgccttg gacgaggagt tccttaaaca ggccagggcg cgggggctaa cccagctcaa
    10021 gggccaccgc tcggtgggcg gcatgcgcgc ttctatttac aacgccatgc cggtggaagg
    10081 ggtcagggcg ctggtccaat tcatgcgcga gttcgaagcc cgacatggct gaccctgtgc
    10141 gcatcctgac actcaattcg atttcccagg tcggcttgaa gcggttgccg gcggagcgct
    10201 ataccgtggg cacgcacgtg cagtcgccgg acgcgatcct ggtgcgatcg caggacatgc
    10261 acgcgatgga cattgcgcag tcggtgaagg cgatcggccg cgccggagcc ggtaccaaca
    10321 atattccggt tgcggcaatg aacgagcgcg gtgtaccggt gttcaatgct cccggcgcca
    10381 atgccaatgc ggtcaaggaa ttggtaattg caggcatgtt gatggcggcg cgcaatctgg
    10441 ttgccgccat ccgctttgtc gacgcgcttt ccgtaaccga cgaggagatc aacaagaagg
    10501 tcgaagaagg caagaagcac tacaccggtg tcgagctgcc acaccatgtg ctcggtatca
    10561 tcgggctggg caggatcggc agcctggtcg cagatgccgc gatcaagctg ggcatgaacg
    10621 tccacggcta cgatcctgaa atcacggtgg acgctgcctg gagcctgccg tcccaggtca
    10681 agaaggcgca cagcatcgag gaagtgatgc gtaacagcga cttcgtaacc atccatgtac
    10741 ccctgctgga agaaacccgc aatctgatcg aagccaaacg catcggtctg atgaagcacg
    10801 gcgcggtgct gctcaatttt tcccgggaaa gcatcgtcga caacgctgcg gtgctgcagg
    10861 ctctggacgc caggcgcctc aaggcctacg tctgcgattt ccccagcggc agcttccgcg
    10921 ggcattccgg cgtagtcgcg ctgccgcacc tgggcgcttc gacccgtgaa gccgaggaca
    10981 actgtgcggt gatggtggtc gagcaggtac gcgattatct ggaaaacggc aacgtccgca
    11041 acgcggtgaa ttttccggac gtgaccgtgg tgcgcgagtc cccgttccgt ctcggcatcg
    11101 ccaatgcgaa cgtgcccaac atggtggggc agatttctac cgctgtcgcg caggccggcc
    11161 ttaatattca caacatgctc aacaagtcga aaaaagacat ggcgtacacg ctggtagatg
    11221 tggacagccc ggtaccctcg aaagtcatcg acaagattgc cgcgatcgaa ggcgtgctcg
    11281 cggtgcgcta cctcccctcg gaggcgtcgt aatgtcggat gagctcaagc aactgcgcag
    11341 ccgcatcgat gcgctggacc aggaaatcct gagattgatc agcgagcgcg cggcggcagc
    11401 gcatgccatc ggcaagctca aaggcgatgg cccggtctat cgacccgaac gcgaggccca
    11461 ggtgctgagg caactcgccg atcgcaatcc cgggccgttg ccggaccagg cggtgatgta
    11521 cctgttcacg gaaatcattt ccgcgtgccg ggcgctggaa gacgcgtttt ccgttgcctg
    11581 cctggggccg aagggcacgt tcagcgagga agcggtgata aagcacttcg gcagccaggc
    11641 gccggcgacg ctttgcggtt cgatagacga agtctttcgc gccgtcgagg ccggcagtgt
    11701 cggatatggg gtcgtgccgg tggaaaactc caccgagggc gcggtgggac gtactctcga
    11761 caacctgctg tccacgccgc tcaagatctg cggggaggtg gtgctgccgg tgcaccagaa
    11821 tctgttgtcc agggctgcga gtatggccgc ggtcaagcgg gtttactcgc acgcgcaaag
    11881 cctcgcgcag tgcagccgct ggttgagcca gaactgcccg cgtgccgaac tggtgccggt
    11941 agcaagcaac gccgaggccg cacgcatggc ggcaaccgag gccggtgccg cggccatcgc
    12001 cggccattca gcggccaaac actacggcct gccggtgctg gcagccaata tcgaggacga
    12061 gccgaacaac accacgcggt ttgccgtgat ctccagacag gaagcgccgc cttccggcaa
    12121 cgacaagacg tcgttggtga tgtccacccg caatatcccg ggcgcgatac acgagttgtt
    12181 gacgccgttg gcgcgctgcg gggtaagcat gacgcggctg gaatcgcgtc catcgcgtac
    12241 cgggttgtgg gaatacgtgt tcttcgtaga ccttgaaggg caccagactg aggacaaggt
    12301 tgcacgtgcg ttggacgaaa tgcgcgggat cgcagctttt ctcaagatcc tcggttcttt
    12361 tccagtggcc gcacaatgaa taccttgtcg tcgctgcagg atatcgtcga cgtctgcgag
    12421 cgttcgccgt cgtatgtccg cgccattgcc ccgtatcagc cgggcaaacc catctcggag
    12481 ctggcgcggg agatcggatt ggaggagggg agcattatca agctggcctc caacgagaac
    12541 ccgcttggcg caagcccgaa ggcgctggcg gcgatgcgtg cggcgctcga cgaagtggcg
    12601 cgttatcccg acggtaacgg tttcgaactg cgcacggcgc tggcgaaaat gtatggcgtc
    12661 gcggcggcgc gaatcgtgct cggcaacggc tcgaacgacg tgctggaaat ggcggcgcac
    12721 gccttccttg agccgggcgc gtccgcagta tattcgcagc atgcgttcgc ggtgtatccg
    12781 ctggcgacgc aggccaccgg cgccgcggga atcgaagtgc cggcgcggga tttcggccac
    12841 gatctggacg caatggggcg tgccgttcgc ggcgacacgc gcgtcgtttt cgtcgccaat
    12901 ccgaacaacc cgaccggcac ctggattgcc gcggaccggc tgcacgcctt cctgcgcaag
    12961 ctgccggcgc acgtgctgct ggtgctcgac gaggcttatt acgaatacct cgatcccggg
    13021 ctgcgcagtg acagcctcgg atggttggaa cagtttccca acctggtcgt ctcgcgcacc
    13081 ttctcgaagg cttacgggct cgctgggctt cgggtcggct tcgcgttcgc ccacccgcag
    13141 gttgccggcc tgatgaaccg cgtcagacag ccgttcaacg tcagcagcat cgcccaggcg
    13201 gccgctgtgg cggcgctggc cgaccaggaa ttcgtgcaca aaagccgcgc gctcaacgct
    13261 caggggatgc tggcgctgac caccggcttc aagcggctgg ggctgtcctg gattccgtcc
    13321 tacgccaatt tcgtcagctt caaggtgccg aacaccgccg aagttttcca gcgcctgctc
    13381 aggcgaggtg tgatcgtgcg cccgatcgcc ggttacggca tgcccgaaca tctgcgcgtg
    13441 acggtcggaa ccggggcgga gaacgcacgc tttctcgccg agcttgaagc ggtcttgacg
    13501 gacctgcgct aaggttccgc caacgcgcgc cgaaatttac ttgagtaatt tccatagtga
    13561 cagattggtc gtcatcggtg tcggcctgat cggcggatcg gtggcggctg cgctcaggcg
    13621 cgccggaaag gtgcgccgcg tgatcggcgt cggccgcggc cgcgccaaca tcgcccgggc
    13681 gcttgaactc ggcgtgatcg acgatgcggt cgaggacatc gcggctgcgg tcaagggggc
    13741 cgacgcggtt ctgatcgcgg tgccggtgca gcagaatccg cacgtgctgg gtgcgttcgc
    13801 cgatgccctg gcgccgggaa cgctggtcac cgacgcgggc agcaccaagc aggatttcgt
    13861 cgccgcagtg cggcgcatcg ccccccggca tcttgcatcc gtcgtaccgg gacatcccat
    13921 cgccggcgcc gaactgaccg gcgtggatgc cgccagcgcc gcccttttcg atggcaggaa
    13981 cgtggtgctc acgcctctcg aggaaaacca ggccgccgtg gtcgatcgcg tagaggcgat
    14041 gtggcaggcc tgcggcgccc gggtgagccg aatgaccccg gaacgtcacg accgcgtgtt
    14101 ctccgcggtg agccatcttc cgcacatgct cgcctatacg ctggtgcaca tgatcgcgac
    14161 ccgggcggac gcggaagcgc tgttcggttt cgccgccagt ggcttccgcg acttcacccg
    14221 catcgccggc agctctgcgg aaatgtggcg cgacatagcg cttgccaata gagatgcctt
    14281 gttggccgac atcgaggctt accagcagga gttggccgag ctggcgcaat gcctgcgtca
    14341 ggctgatggc gcggaaatcg agcgcatatt cgaggcggcg cgctgcgcgc gcaatgcctg
    14401 gatacgcaac ggctcgtgag gcatggccgc ctatcctgag tacctggaac ttggaccgtt
    14461 cgaccgggcg acggggaggt tgcgcctgcc gggatcgaag agcatttcta accgcgtctt
    14521 actgctggcc gcgctcgggc acgggatcac cggtgttcac gatctgctgt cggcggatga
    14581 cgtgaatcgc atgctcgaag cgctggaaaa actgggcgtg aagttgcaga aggggaaaga
    14641 gccggggcac ctgagcatca acggttgcgg cggtacgttt ccggtgaaaa gcgccgaact
    14701 gtttctcggc aatgccggca ccgccttgcg gccgctgact gctgcgctgg cgctttgcgg
    14761 cggacattac cggctgtccg gcgtaccgcg aatgcacgag cgtccgatcg gcgatctggt
    14821 ggacgcgttg cgccaggtcg gcgccaacgt gcgttacttg gggaaggagg gcttcccgcc
    14881 gctggaaatc cgggctggaa atatcgacga tgcaggcaag gtgcgggtga gcggcgaggt
    14941 ttccagccag ttcctcactg cgctgctgat ggcgttgccg ctgaccggca agcaaacgac
    15001 gatcgaagtc gtcggcgatc tgatttccaa accctacgtc gagatcacgc tcaacctcat
    15061 ggcgcggttc ggcgtgcaag tgcgccgcgc gggctggtcc tcgttcacga ttcctgccga
    15121 cagccgcttt cacagtcccg gg
//
LOCUS       DASQOP010000090         3525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_437164, whole genome shotgun sequence.
ACCESSION   DASQOP010000090 DASQOP010000000
VERSION     DASQOP010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437164"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..1014
                     /locus_tag="VE030_03580"
     CDS             247..1014
                     /locus_tag="VE030_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZF22530.1"
                     /translation="MGEMLAVSGLTKVFGGFTAVNSVSFEVKEGEILGLIGPNGSGKS
                     TTFNLIAGALAPTAGSIRFLGRETCGWPAYRVAHAGIGRTFQIPRPFRRLSIIENVAL
                     AACYGQADRPTRQQAFSQAKEILDLVRLSAEPGSRIEGLGAAGLKKLELARALATKPK
                     LLLADESLGGLDEQEMNQAADLLAEIRKQCGITIVWVEHIMGALMRVVDRCVVLDHGE
                     VIAQGKPEVVAHDPRVIEVYLGTDAKDVQATVAARSN"
     gene            1014..1718
                     /locus_tag="VE030_03585"
     CDS             1014..1718
                     /locus_tag="VE030_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZF22531.1"
                     /translation="MLTLRNISAGYAGFQALFDLSLEIKAGETVAVIGPNGAGKTTLL
                     RVVSRLIDATAGEMTMEGRRLNDVASYEVVSHGIAHVPENRRLFPKLTVEENLRMGAF
                     VPQARAHFEGRLARVYQLFPRMEERRRQRAGTLSGGEQQMCAIGRALMSGPKLLLLDE
                     PSAGLAPVVVQSIFETVRRMSAEGYTVLIVEQNIRQVLKVASRGYVLETGRIRAAGTA
                     AELLDTDDIRKAYLGL"
     gene            1943..2812
                     /locus_tag="VE030_03590"
     CDS             1943..2812
                     /locus_tag="VE030_03590"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZF22532.1"
                     /translation="MDIFDIFLLEAIVNGVLLGGILALLALGLNLIFGVIDVVWICYA
                     ELIMIGMYTAFYLHVDYGWPLWLAFPGCVVVVSALGVALHHFVIRPVLTAEPINQLLV
                     TGGVLFFLQGAATLAFGVEFRNIGVKLPTFELGGMFLSLARLTAFLIALASMIGVYLF
                     LKRTYLGTAIRAISQDRTIMPLMGVNPSRIFLVTSALGGALAGVAACLLVLQYDIHPA
                     IGLSFGPIAFLVCVLGGLGNMIGGFLAAFIFAQFISVGGYFFHLEWGYVLAFLFFIAF
                     MFWRPQGLLGGKS"
     gene            2809..>3525
                     /locus_tag="VE030_03595"
     CDS             2809..>3525
                     /locus_tag="VE030_03595"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZF22533.1"
                     /translation="MRRALPWIGVALLAAAPLTGMGNYPLHLMIMCLLWAFVYTSWAL
                     MGRLGMTSLGHGAFLAVGAYGVVMAWNLFGLSPWLSLPVLLLFAALTAFLIGYPCFRL
                     KIVGHYFALVTLALGEIARMVIVALRDQTGGSLGATPNTALADASWSLTALQFSSKLV
                     WFYLILGFWLFGLWVWTRVDRSMARLALEAISQEEDAAASIGINVTRLKLGVTVISAL
                     MTCLGGALYAQYQLYVNPETV"
BASE COUNT          600 a         1064 c         1140 g          721 t
ORIGIN      
        1 cagaacgaac gctcctcgcc ggcggtgatg cagtatatcg gcggcaagac cgaagtcgtt
       61 tggcccgccc ggctgcgcac cgcagatccg gtgctgccct ggccgaaggc gcacagctat
      121 tcgaaccagt agtgccgtga atgtcgtgaa tatcgtgaat gccgtgacaa attagtggtc
      181 acattgtgtg acgttgttgc gatattcacg acattcccgg catttgcgat attcacggaa
      241 cttcaaatgg gggagatgtt agcggtcagc ggattgacca aggtctttgg cggcttcacg
      301 gccgtcaaca gcgtttcctt cgaggtgaag gaaggcgaga tcctgggctt gatcggcccg
      361 aacggctcgg gcaagagcac cactttcaat ctcatcgccg gcgcccttgc accgaccgcg
      421 ggctcgatcc gctttctcgg ccgcgagacc tgcggttggc cggcttaccg cgtcgcccac
      481 gccgggatcg ggcggacgtt ccaaatcccg cgccctttcc gccggctgtc gatcatcgag
      541 aacgtcgcgc tcgccgcctg ctacggccag gccgatcgcc cgacgcgcca acaagcgttc
      601 tcccaggcaa aagagatcct cgatctggtg cgcctgagcg ccgaacccgg ctcgcgcatc
      661 gaaggtctgg gcgccgcggg gctgaagaaa ctggaactgg cgcgcgcact cgccaccaaa
      721 ccaaagttgc ttcttgcgga cgagtcgctc ggcggcctcg acgaacagga gatgaatcag
      781 gccgcggacc tgctcgcgga gatccgaaag caatgcggca ttaccatcgt ctgggtcgag
      841 cacatcatgg gcgcgctgat gcgggtggtg gatcgctgcg tggtgctgga ccatggcgaa
      901 gtgatcgcgc aaggaaaacc cgaggtggtg gcacacgacc ccagggtgat cgaagtgtat
      961 ctgggcaccg acgccaaaga cgtgcaggca accgtggccg cgcggagcaa ctgatgctca
     1021 cgctgcgcaa catatccgcg ggctacgccg gattccaggc gctgttcgac ttaagcctcg
     1081 agatcaaggc aggggaaacc gtcgcggtga tcgggccgaa cggtgccggc aagacgacgc
     1141 tgctcagagt ggtctcgcgg ctgatcgatg cgaccgcggg cgagatgacc atggaaggcc
     1201 ggcggctgaa cgatgtggcc tcatacgaag tggtcagcca cggcatcgcg cacgtgccgg
     1261 agaaccggcg actgttcccg aagctcactg tcgaggagaa cctgcgaatg ggcgcattcg
     1321 tgccgcaggc gcgcgcgcat ttcgaagggc ggctggcgcg cgtgtaccag ttgtttccgc
     1381 gaatggaaga gcgccgccgc cagcgggccg gcacactttc cggcggcgag cagcagatgt
     1441 gcgcgatcgg ccgcgcgctc atgtccgggc cgaagctgct tctgctcgac gaaccctccg
     1501 cgggactggc gcccgtggtg gtgcagtcga tctttgaaac ggtgcggcga atgagcgcgg
     1561 agggctacac cgttctgatt gtcgagcaga acatccgcca agtgctcaaa gttgcgagtc
     1621 gcggttatgt gctggagacg gggcgcatcc gcgccgcagg aacagccgcg gagctactcg
     1681 acaccgatga catcaggaag gcttacctcg gactttagaa gaaaaagcgc caaacgcaga
     1741 ggacgcgaag gacgcggagg aaagcatgaa aagtgatcga atccagaaga ttgccaatgt
     1801 cccggatggg gagaggtttc cattattccc ttggccgcgt ggtcaagaat ttccttcacg
     1861 catctctgcg tcctctgcga cctctgcgtt gagagctatc gattttccgt cgcccggaaa
     1921 cccgcacacg acccaataga caatggatat tttcgacatc tttctgctgg aagccatcgt
     1981 caacggcgtg ctcctgggtg gcatcctggc gttgctggcg ctgggtctga acttgatctt
     2041 cggcgtgatc gacgtggtgt ggatctgtta cgccgagctg atcatgatcg gcatgtacac
     2101 agccttctat ctgcacgtgg actacggctg gccgctgtgg ctcgcctttc cggggtgcgt
     2161 cgtagtggtc tcggcgctgg gcgtggcgtt gcaccacttc gtcatccgcc cggtgcttac
     2221 cgccgagccg atcaaccaac tgctggtcac cggcggcgtg ctgtttttcc tgcagggtgc
     2281 ggccacgctg gccttcggtg tcgagttcag gaacatcggc gtaaagctgc cgaccttcga
     2341 gctgggcggg atgttcctga gcctggcgcg gttgaccgcg ttcctgatag cgctggcaag
     2401 catgatcggc gtctatctgt tcctcaagcg cacttacctc ggaactgcga tccgcgccat
     2461 ctcgcaggac cgcaccatca tgccgcttat gggcgtgaac ccgagccgca tcttcctggt
     2521 cacgtccgcg ctgggcggcg cgcttgccgg agtcgccgcc tgcctgctgg tgctgcaata
     2581 cgacattcac ccggcaatcg gattgtcttt cgggccgatc gccttcctgg tttgcgtgct
     2641 cggcgggctg ggcaacatga tcggcggctt tctcgcggcg ttcatcttcg cgcaattcat
     2701 ctcggtgggc gggtactttt tccacctcga atggggctac gtgctggctt tcctgttctt
     2761 catcgccttc atgttctggc ggccgcaggg cctgctggga ggcaagtcat gaggcgcgcc
     2821 ttgccctgga tcggtgtcgc actactggcg gcggcaccgc tcacgggtat gggcaactac
     2881 ccgctgcacc tgatgatcat gtgtctgttg tgggcgttcg tgtacacgag ttgggcgctg
     2941 atgggacggc tcggaatgac cagcctcggc cacggcgcct ttctcgccgt cggtgcatac
     3001 ggagtggtga tggcatggaa tcttttcggg ctctcgccct ggctgtccct gccggttttg
     3061 ctgttgttcg ccgcgctgac cgcgttcctg atcggctacc cgtgtttccg cctgaagatc
     3121 gtcggccact attttgcgtt ggtgaccctt gccctcgggg aaatcgcgcg catggttatc
     3181 gtcgcgctgc gcgaccagac cggcggctcg ctcggtgcca cgcccaacac cgcgctcgcg
     3241 gacgcgtcct ggtcgctcac cgcgctgcag ttttcctcca agctggtgtg gttctatttg
     3301 atcctcggct tctggttgtt cggactgtgg gtgtggacgc gggtggatcg ctcgatggcg
     3361 cggctcgcgc tggaggcgat cagtcaggag gaagacgccg ccgcatcgat cggcatcaac
     3421 gtcacccggc tcaagctcgg agtgacggtg atctcggcgc tgatgacctg cctgggcggc
     3481 gcgctgtacg cgcagtacca gctctatgtg aatcccgaga cggtt
//
LOCUS       DASQOP010000091         3971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2047171, whole genome shotgun sequence.
ACCESSION   DASQOP010000091 DASQOP010000000
VERSION     DASQOP010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2047171"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VE030_03600"
     CDS             <1..328
                     /locus_tag="VE030_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZF22534.1"
                     /translation="YHWVDYPSAERELEIVRRKVPGVAMQLSREVVAFVQQLRAVDLF
                     KLPGVAETIDWSRALSALDRVALDPQAINDTLGVLLKYEDDIARVRGDPVAGLLEKAK
                     AELPAA"
     gene            381..1571
                     /locus_tag="VE030_03605"
     CDS             381..1571
                     /locus_tag="VE030_03605"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZF22535.1"
                     /translation="MTRVPAEGKLSQNVMHFAQALRRAGVAVGPDRVLDSLRALEVTG
                     VARREDFYWTLASVFLSRREQFELFDQAFQLFWSNRLLPESAVAGLPLAAPSTGPDRE
                     APPGRLADALGLTRKARLERESPRRADEAESAAGASQIEALRRRDFETMTTDELMQAK
                     KLIAGLRLPIPAIGTRRLQPDSRGRFVDLRGTLRASLRGNADVILLRRRSHQHRHPPL
                     VILCDISGSMSRYSRMFLHFTHAITNDRDRVHTFVFGTRLTNITRHLRHRDVDKALGA
                     VSLAVADWSGGTRIGATLKEFNLRWSRRVLGQNAEVLLISDGLDRDAGLGLPQAMERL
                     HKSCRKLIWLNPLLRFDGFEPKTTGVRLMLPHVDAFVPAHSVESLADLARFLAPAQQD
                     QRRQ"
     gene            1575..2135
                     /locus_tag="VE030_03610"
     CDS             1575..2135
                     /locus_tag="VE030_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HZF22536.1"
                     /translation="MEMTGEQLIPLPQQRVWESLNDPGILKACIPGCESIEKVSDTEY
                     KVVMTAAIGPVKAKFNGKLLLADLNPPHSYSIAFEGTGGAAGFGKGAARVSLLPEGNG
                     TRLSYKATASVGGKLAQIGSRLIDGVAKKMSDDFFARFNQVVAPAGTPAAAPAMPMGR
                     KGIPVLAWLVVGIVVLLTLLILFKIV"
     gene            2132..3313
                     /locus_tag="VE030_03615"
     CDS             2132..3313
                     /locus_tag="VE030_03615"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZF22537.1"
                     /translation="MRRDESSVDVAIIGAGAAGMMCAIEAGKRGRRVLLLEHAAKLGE
                     KIRISGGGRCNFTNIESRPENFLSANPDFCRSALARYTPRDFIALVEKHGIAWHEKKL
                     GQLFCEGSSRQIIDMLKAECDDASVRWCMPAKVISVTKDERFSIATDRGDFESESLVI
                     ACGGLSIPQIGASPFGYRIAEQFGLEVTELRPALVGLTFAPETLTRFAGLSGISIDAI
                     VGCNGTRFRENLLVTHRGLSGPAILQISSYWRPGLDLSVNLLPDLDAREMLEAAHSSE
                     KMLANVLGALLPQRFAQRWIEINFENLPMKRLGASRLREIAGRLRDWRLRPSGTVGYK
                     KAEVTLGGVDTRELSSRTLEARKTPGLYFIGEVVDVTGHLGGHNFQWAWASGYAAGQA
                     V"
     gene            3663..>3971
                     /locus_tag="VE030_03620"
     CDS             3663..>3971
                     /locus_tag="VE030_03620"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZF22538.1"
                     /translation="MNQLDMKGRSAIVTGGASGIGLAIARRLAQSGANVAIWDMNEKT
                     LADAAKSLGAPKTHTARVDVTKLDQIEAAFKGTVAAFGKVDALVCSAGIAGMNKVTWE
                     L"
BASE COUNT          729 a         1276 c         1251 g          715 t
ORIGIN      
        1 ctatcactgg gtggattatc cgtccgccga acgggaactg gagatcgtca gacgcaaagt
       61 cccgggcgtg gccatgcagt tgtcacgcga ggtggtggcc ttcgtgcagc aattgcgcgc
      121 cgtcgatctg ttcaagctgc ccggcgtcgc ggaaaccatc gactggtcca gggccttgag
      181 cgcacttgac cgcgtggcgc tggatccaca ggcgatcaac gacacgctgg gcgttctgct
      241 gaaatatgag gacgacattg cccgtgtgcg cggcgacccg gttgcgggcc tgctcgagaa
      301 agccaaggcg gagctacctg ccgcctgatg cgtccgtgtg tgcggacgcc ccccgcgcga
      361 tgcgttcaga acctcgaccc atgacccgcg ttcccgccga aggcaagctg tcgcagaacg
      421 tcatgcattt tgcccaggcc ctgcgccggg ccggtgtcgc cgtgggaccg gatcgggttc
      481 tggattccct gcgggcactg gaagttaccg gcgttgcgcg gcgcgaggac ttctactgga
      541 cgctggcttc ggtgtttctc agccgccgcg agcaattcga gctgttcgac caggcattcc
      601 agctgttctg gtcaaaccgg ctgctacccg aatcggccgt ggccggtctg ccgctggcgg
      661 ccccttcaac gggaccggat cgcgaggcac ctccgggccg tctggcagac gcactcggac
      721 tgacccgcaa ggcccgcctc gaacgcgaat cgccgagacg cgcggacgaa gcggaatccg
      781 cggccggagc ctcgcagatc gaggcgctgc ggcgccggga cttcgagacc atgaccaccg
      841 acgaattgat gcaggcgaag aagctcattg ccggcctgcg cctgccgatc ccggccatcg
      901 gcacgcgccg gctgcagcct gactcgcgcg gccggtttgt ggacttgcgc ggcacgctgc
      961 gcgccagcct gcgcggcaac gcggacgtca tccttttgcg ccgccgttcg caccagcacc
     1021 gccatccgcc gctcgtgatc ctctgcgaca tctccggctc gatgagccgg tactcacgca
     1081 tgttcctgca tttcacgcat gcgatcacca acgaccgcga ccgcgtgcat actttcgtct
     1141 tcggcacccg gttgacgaac atcactcgtc atctccggca tcgcgatgtg gataaggcac
     1201 ttggcgcggt gtccttggca gtcgccgact ggtcaggcgg cacacgcatc ggcgcgacgt
     1261 tgaaggagtt caacctgcgc tggtcgcgcc gcgtgttggg ccagaacgcg gaagtgctac
     1321 tgatcagcga cggcctcgat cgcgacgccg gcctgggcct tccacaagcc atggagcggc
     1381 tgcacaagtc ctgccgcaag ctgatctggt tgaatccgct gctgcgtttc gacggcttcg
     1441 agccgaagac caccggcgtg cgcttgatgc ttccgcacgt ggatgcattc gtcccggcgc
     1501 acagcgtcga gagcctggca gaccttgctc ggtttctggc accggcgcaa caggatcaaa
     1561 ggagacaata aataatggaa atgaccggcg agcagctcat ccctcttccg cagcaacggg
     1621 tgtgggagtc gctcaacgat cccgggatcc tcaaggcctg catccccggc tgcgagagca
     1681 tcgagaaggt ctcggacact gaatacaagg tggtgatgac tgccgcgatc ggcccggtca
     1741 aagcgaagtt caacggtaaa ctgctgctcg cggacctgaa cccgccccac tcctactcga
     1801 tcgctttcga ggggacgggc ggcgcagcag gtttcggcaa aggagccgcg cgggtctcat
     1861 tgctgcccga gggcaacggc acacggctca gctacaaggc caccgcctcg gtcggcggca
     1921 agctcgctca gatcggctcg cggctgatcg acggtgtagc gaagaaaatg tccgatgatt
     1981 tcttcgccag gttcaaccag gttgtcgctc ccgccggcac acccgcggca gctccggcga
     2041 tgccgatggg ccgcaaggga atacccgtcc tggcgtggct tgtcgtcgga atcgtggttt
     2101 tgctgacact cctgatcctg ttcaagatcg tgtgaggcgc gacgaatcga gcgtcgacgt
     2161 cgccatcatc ggcgccggcg cagccggcat gatgtgcgca atcgaagcgg gcaagcgggg
     2221 ccgccgcgtg ctgctgctgg aacacgccgc caaactcggg gaaaagatcc gcatctccgg
     2281 cggcggccgc tgcaatttca ccaacatcga atcgcggccc gagaacttcc tgtcggcgaa
     2341 cccggatttc tgccgttccg ccctggcgcg ctacacgccg cgcgacttta tcgcgctggt
     2401 ggaaaagcac ggaatcgcct ggcacgagaa aaaactcggc cagctattct gcgaaggctc
     2461 atcccggcag atcatcgaca tgctgaaggc cgaatgcgat gacgccagcg tacgctggtg
     2521 catgccggcc aaggtgattt ccgtgacaaa agacgaacgc ttttcgatcg cgaccgaccg
     2581 cggcgacttc gaaagcgaat cgctggtgat cgcctgcggc ggactgtcga taccgcagat
     2641 cggcgccagc cccttcggtt atcgcatcgc ggagcagttc ggcctcgaag tcacggaact
     2701 gcgcccggcg ctggtcgggc tgacctttgc ccccgagacc ctgacgcggt tcgcgggctt
     2761 gtccggcatt tccatcgacg ccatcgtcgg ctgcaatggc acgcgcttcc gcgagaacct
     2821 gctggtcacc caccgcggac tttccggtcc cgccatcctg cagatttcct cctattggcg
     2881 gccgggcctg gacctgtcgg tgaatctgct gccggatctc gacgcccggg aaatgctcga
     2941 ggcggcgcat tcttccgaaa agatgctcgc caacgtgctc ggcgcgttgc ttccgcagcg
     3001 tttcgcccag cgctggatcg agatcaattt cgagaacctg ccgatgaaac ggctgggcgc
     3061 aagccggctg cgcgagatcg ccggacgcct gcgtgactgg cgactcaggc cgagcggcac
     3121 ggtcggctac aagaaagccg aggtcaccct cggtggcgtg gatacacggg agctatcgtc
     3181 caggaccctc gaagcgcgca aaacgccggg actgtatttc atcggcgaag tggtggacgt
     3241 taccggtcac ctcggcggcc acaacttcca gtgggcgtgg gcctccggtt acgcggccgg
     3301 ccaggcggtc tgaaacgcgc tcaccgcaga ggacgcggag gaaaggcagg aggaaaaaac
     3361 attctcgtgt taaaaccgtt cattggctga tgatccatcg cggtgaatat tggcggtaga
     3421 gatccaggaa cagctacgcc gcttttaatc ccagattgct tttcctcgtt cttgttttcc
     3481 ctccgcgtcc tctgcgtcct ctgcggtgag cgcttgtaaa gctttaggcg gtgaacgctt
     3541 atctaaaaat gggtttggtc gcggccgtcc gcctgaatta aaatgccggc tttccgttgc
     3601 gggcgatcgc aaccgcgcgg tcactattcc aatccaaagg gtccaatcca aaggggggag
     3661 caatgaatca actcgacatg aagggccgca gcgccatcgt aaccggcggg gcttccggca
     3721 tcggactggc catcgccagg cggctcgcgc agtcgggcgc caacgtcgcc atctgggaca
     3781 tgaacgagaa gaccttggcg gacgccgcca agtccctcgg cgcgccgaag acccatacag
     3841 ccagggtgga cgtcaccaag ctcgaccaga tcgaagccgc attcaagggc accgttgcgg
     3901 cgttcggcaa ggtcgacgcc ctggtgtgct ccgcaggcat cgccggcatg aacaaggtca
     3961 cctgggaatt g
//
LOCUS       DASQOP010000092         9864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_636224, whole genome shotgun sequence.
ACCESSION   DASQOP010000092 DASQOP010000000
VERSION     DASQOP010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9864
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636224"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(243..1439)
                     /gene="tyrS"
                     /locus_tag="VE030_03625"
     CDS             complement(243..1439)
                     /gene="tyrS"
                     /locus_tag="VE030_03625"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018054613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HZF22539.1"
                     /translation="MEIKEQLEIIKRGSDELLVEQELVDKLKSGHPLRVKAGFDPTAP
                     DLHLGHTVLINKLRQLQDLDHHILFLIGDFTGMIGDPTGKNATRPPLSREQITQNAET
                     YTTQVFKILDPNKTEVCFNSSWMDKLSAADMIKLAATHTVARMLERDDFGKRYKSNQP
                     IAIHEFLYPLVQGYDSVAMRSDMELGGTDQKFNLLMGRELQKLWGQSPQCVLTMPLLE
                     GLDGVNKMSKSLGNYIGIAESPEQIFGKIMSISDELMWRYIELLSFESLATIRKWEQE
                     VKAGRNPRDVKVTFAKEIVARFHDRAAAAKALADFEARFKQGEIPDNIPEVTLTTGGE
                     PMLFYQVLKKAGLTASTSEAIRMIEQGGVKLNGEKVSDKALKLATGGPFVLQVGKRKF
                     AKVLLS"
     gene            1593..2915
                     /locus_tag="VE030_03630"
     CDS             1593..2915
                     /locus_tag="VE030_03630"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZF22540.1"
                     /translation="MLLNKEEILAQKHRPEIRRTRLHWPLVVAALPFFGVVAAFGIAP
                     DTVPEPIDLQHVIEDIALPSGPTTGTDELARFWREERIQRGDTVASVLARLKVNDPAA
                     VAYLLQAKDVRSLYKLIPGRSIRVVTAEGGGLESLSYVNTDGRRLHVKRTEPGFSASE
                     EVPPTEQWVMQSSGEIETSLFAATDVAGVPEMVAVQLAEIFSSDVDFHRDLRRGDRFS
                     VVYEALAADGEFVGFGRVLSAEFVNQGNVFRAVYFRDAQGRSGYYTPDGRNVRKAFLR
                     SPIEFSRVTSGFSNSRFHPVLKTWRAHKGIDYGAPTGTRVRTTADGYVTFAGSKGGYG
                     NVVTVRHPNGYTTLYAHLSGIAQGMRQGRRVIQGEIVGFVGSTGLATGPHLHYEFLVN
                     GVHHNPMRLAMPPGPPITAQLRSEFEEAARPLLARLDMLRDTNLARLD"
     gene            2973..4070
                     /locus_tag="VE030_03635"
     CDS             2973..4070
                     /locus_tag="VE030_03635"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HZF22541.1"
                     /translation="MAGNERFIGLMSGTSLDGVDAVLMDFSGNANRVVGNTFIPYDDR
                     LRAQLLALHQPHTGELHNAAVLGNQLARMYAQAVDELLRKTKVVRQDIVAIGCHGQTI
                     RHRPDAGYTIQLGNGALLAELSGITVVCDFRGRDIAAGGEGAPLVPAFHTVVFSHLSV
                     HRVVVNIGGIANLSDLPPSGDVRGFDTGPGNALMDAWIQRHLGKPFDKDGAWSRSGLP
                     DDKLLNALLAHEFFSRKPPKSTGRETFHLQWVEQYLTGREKPEDVQATLLALTATSIA
                     SSVRSYCAGTREIYLCGGGAQNGALIAILEAMLPGVLIARTDELGIDADWVEACAFAW
                     LARQTLQRRPGNLPGVTGARGPRILGAIYPN"
     gene            complement(4120..4470)
                     /gene="erpA"
                     /locus_tag="VE030_03640"
     CDS             complement(4120..4470)
                     /gene="erpA"
                     /locus_tag="VE030_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HZF22542.1"
                     /translation="MNAVTEMPSPLVFTDSAATKVKELIDEEGNPDLKLRVFVTGGGC
                     SGFQYGFTFDEAVNEDDTSMEKNGVMLLIDAMSFQYLAGAEIDYQEGIEGAQFVIKNP
                     NATSTCGCGSSFSA"
     gene            complement(4529..4936)
                     /locus_tag="VE030_03645"
     CDS             complement(4529..4936)
                     /locus_tag="VE030_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HZF22543.1"
                     /translation="MFGAKPTKPQNRIDSLIGTGTVVEGNISFTGGLRVDGRVRGNVA
                     ATDDQPSTLVLSEKAQIEGEIRVSHAVINGTVIGPVHGAEYVELQPKSNVTGDVHYKT
                     LEIQLGAVVQGRLVHQDDDKAQKVVSLKGSSGD"
     gene            complement(4950..5693)
                     /locus_tag="VE030_03650"
     CDS             complement(4950..5693)
                     /locus_tag="VE030_03650"
                     /inference="COORDINATES: protein motif:HMM:NF042896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HZF22544.1"
                     /translation="MTLIRTIKRRFGIAAPRVAIHTHVPWYWRWLSIIVVSGLALGVA
                     WLTYDFGRQYAGFDQGEAQREKARLEDLNSNLKDENASLRREIAAAERQLQIELATHG
                     NLSAQIHTLAEENALLKEDLAFFQTLMASGSEPGGVSVNRFRVQPDALPGEYRYRLLI
                     VQSKQRVREFRGHIQFIVDLEDGGKAEVISLPREGESAQAYNLVFKFYQRVEGTFVVA
                     PGAVVKKVQVRVLENGNPTPVSTQTVALS"
     gene            complement(5690..6718)
                     /gene="argC"
                     /locus_tag="VE030_03655"
     CDS             complement(5690..6718)
                     /gene="argC"
                     /locus_tag="VE030_03655"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HZF22545.1"
                     /translation="MIKVGIVGGTGYTGVELLRILATHPGVELAAITSRKEAGMLVAD
                     LFPSLRKRVSLKFSDPATAQLEKCDVVFFATPNGVAMEQAPALLKAGVRVIDIAADFR
                     IKDIAEWQKWYGMTHACPEVVAEAVYGLPEVNRDLIKKARVVANPGCYPTSVQLGFLP
                     LVEAGVVDIEYLIADAKSGVSGAGKKAEVHILFSEASDNFKAYRVPGHRHLPEIEQGL
                     SRAAGKKVGLTFVPHLTPAIRGIHATLYGRLTKEVDVQALYERRYAAEPFVDVLPPGS
                     HPDTRSVRGANVCRIAVHRPGGGNTVVVLSVIDNLVKGAAGQAVQNMNLMFGMSEATG
                     IEHVALLP"
     gene            complement(6821..7216)
                     /gene="rpsI"
                     /locus_tag="VE030_03660"
     CDS             complement(6821..7216)
                     /gene="rpsI"
                     /locus_tag="VE030_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HZF22546.1"
                     /translation="MATTPYNYGTGRRKSAVARVFIKPGKGSIVVNDKPVDEFFSRET
                     GRMVVRQPLQLTNHLNTFDILINVDGGGESGQAGAVRHGIARALVDYDASLKPVLSKA
                     GLITRDAREVERKKVGLHKARRRKQFSKR"
     gene            complement(7225..7653)
                     /gene="rplM"
                     /locus_tag="VE030_03665"
     CDS             complement(7225..7653)
                     /gene="rplM"
                     /locus_tag="VE030_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HZF22547.1"
                     /translation="MKTFSAKTHEVKRDWFVIDATDKVLGRLAAEVAKRLRGKHKVTF
                     TPHVDTGDYIVVVNAKKLRVTGNKAEQKIYYRHSTYPGGIYSTSFAKLHSRFPGRVLE
                     KAVKGMLPKGPLGYAMLKKLKVYPDGEHPHAAQQPQALNF"
     gene            7932..9095
                     /locus_tag="VE030_03670"
     CDS             7932..9095
                     /locus_tag="VE030_03670"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003263432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HZF22548.1"
                     /translation="MRKFWLIFSQAVTISLAALFVVVTLRPDLVSWSARGGGIVTVRE
                     AADGSGNRANMTSLSPAAKKAMPSVVNIFTSKEVKVPRHPFMDDPLFRRFFGDQLDGD
                     TQHSSSLGSGVIVGPEGYILTNHHVIEAADEIELALADGRKAPARIVGTDPETDLAVL
                     KVDIADLPAVTFGNPQTLSVGDMVLAIGNPFGVGQTVTMGIVSALGRSHLGISTFENF
                     IQTDAAINPGNSGGALVDSAGNLIGINSAIYSRSGGSLGIGFAIPADLAKQVMQQIVG
                     TGSVTRGWIGVEVQDISAELAESFRLSGTKGTLIAGVLRGGPADRAGVKPGDVLVAVE
                     SNPVVDSTNMLNLIAALEPGKQATLKVLRNRAEMLIEVNVGKRPKPSRRHGSE"
     gene            complement(9120..9845)
                     /gene="tatC"
                     /locus_tag="VE030_03675"
     CDS             complement(9120..9845)
                     /gene="tatC"
                     /locus_tag="VE030_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HZF22549.1"
                     /translation="MSHLVELRDRLLRCVIALVLVFICLFPWARNIYSMLAKPLLASL
                     PAGGQLIATEVTAPFFVPIKVTMMAALVIALPYLLYQIWAFVAPGLYAHEKKLVMPLI
                     ITSTVLFLCGMAFAYFLVFPIVFHFIISVAPEGVAVMTDIGKYLDFVLTLFVAFGVTF
                     EVPVAVVILVRMGMVSVAKLRDIRPYVIVGAFVIGAIFTPPDMVSQVMLAVPLWVLYE
                     LGIFVAQWVGKSAAAETRSDPAA"
BASE COUNT         1918 a         3031 c         2854 g         2061 t
ORIGIN      
        1 gtcttcttca agacctaaga accgggcgat gacccaccca ccccgcgcaa gcgccccacc
       61 acacgaagca cccacacgca tcggctgttt gattttttaa agagcgacgc cgttaccggc
      121 ggccgtttcc ggcgaaggtg cgcattatac ggatcaaagc ccgccggtca acacttttcc
      181 gacgaatttt gctgtcggaa cagacggttt cgcccgcgcc gtgcctgggc cgggactgcg
      241 cctcaggaca gcagaacttt cgcgaacttt cgcttgccga cttgaaggac aaacggcccg
      301 ccagtggcca gcttgagggc cttatcgctg accttctcgc cattgagctt cacgccaccc
      361 tgctcgatca tgcgaatcgc ctcagaagtg ctggcggtca ggccggcttt cttcagcacc
      421 tgatagaaga gcatgggttc gccccctgtc gtcaacgtaa cttccgggat gttgtccggg
      481 atctcgccct gcttgaaacg cgcctcgaag tccgcgagcg ccttggccgc ggccgcgcga
      541 tcatggaagc gggcgacgat ctccttggcg aaggtcacct taacgtcgcg cggattcctg
      601 cccgccttga cctcttgctc ccattttctg atggttgcga gcgactcgaa ggacagcaat
      661 tcgatgtagc gccacatcag ctcgtcggaa atcgacatga tcttgccgaa tatctgctcc
      721 gggctttcgg cgatgccgat atagttgccc agtgatttcg acatcttgtt gacgccatcc
      781 aggccttcca gcaagggcat agtgagaacg cactgcggcg actgtcccca aagcttctgc
      841 agctcacgcc ccatcagcag gttgaacttc tggtcggtgc cgccgagttc catgtcggaa
      901 cgcatcgcca ccgagtcgta gccctgcacc agcggataca ggaattcatg aatcgcgatt
      961 ggctgattgc tcttgtagcg cttgccgaag tcgtcgcgct ccagcatgcg cgcgactgta
     1021 tgcgtcgcgg caagtttgat catgtcggcc gcactgagct tgtccatcca gctcgagtta
     1081 aagcagacct cggtcttatt cggatcgagg atcttgaaca cctgcgtggt ataggtttcc
     1141 gcgttctgcg taatctgctc gcgggaaagt ggcgggcggg tcgcgttctt gccggtcggg
     1201 tcgccgatca tccccgtgaa gtcgccgatc aagaacagga tgtgatggtc aagatcctgc
     1261 aactgccgca gcttgttgat gagcaccgtg tgccccagat gcagatcggg cgcggttggg
     1321 tcaaatccgg ctttcacccg cagaggatga ccggacttga gcttatcgac gagttcttgc
     1381 tcgaccagca gctcgtcgct gccgcgcttg atgatttcca gttgttcttt gatttccatg
     1441 ttcggtttgc ggtgatgctt ctggtatccg gaaacgggaa cgattacgct ccaatgcaac
     1501 aacgtgatct ggaacactag ttgcggtctg tgggtttcgc gcagcggggg attctgctaa
     1561 gctccgcccc aatttatctg gaatacggac gcatgttgtt gaacaaagaa gagattctag
     1621 cgcaaaagca tcggcccgag attcgccgga cgcggctaca ctggccgctt gtggtcgccg
     1681 ctttgccttt tttcggcgtg gtggctgcct tcggtatcgc gccggacact gtcccggagc
     1741 cgatcgacct gcaacacgtc atcgaagaca tcgccctgcc ttcggggcct acaaccggta
     1801 ccgatgaact tgcccggttc tggcgcgaag aacgcattca gcgcggcgat actgtggcct
     1861 ccgttctggc gcgcctgaaa gtgaacgatc cggccgcagt cgcgtacctg cttcaggcaa
     1921 aggacgtccg ttctctttac aagctgatcc ccggccgcag cattcgcgtg gtcaccgccg
     1981 agggcggcgg cctggagtcg ctttcctacg tcaacaccga tggccgccgt ctgcacgtaa
     2041 agcgcaccga acccggcttt tctgcctccg aggaagtacc gcccaccgag cagtgggtga
     2101 tgcagagttc gggcgagatc gagacgtcat tgtttgccgc gaccgatgtg gcgggtgttc
     2161 ctgaaatggt cgcggttcaa cttgccgaga ttttttccag cgatgtggat tttcaccgtg
     2221 atctgcggcg cggcgaccgc ttctcggtcg tctacgaagc gctggccgcg gacggtgaat
     2281 tcgtcggttt cggtcgtgtc ctgtcagcgg agttcgtcaa ccagggcaac gttttccgcg
     2341 cagtctactt tcgtgatgcc caaggacgca gcggctacta cacgcccgac ggacgcaacg
     2401 tgcgcaaggc attcctgcgt tcgccgatcg aattctcgcg cgtaacctcg gggttctcca
     2461 attcgcgctt tcatccggtg ctgaaaacct ggcgcgccca taaaggcatc gactacggcg
     2521 cgcctaccgg cacgcgcgtt cgcacaaccg ccgatggtta cgtcacgttc gccggtagca
     2581 agggcggtta cggcaacgtg gtgaccgtgc gtcatcccaa cggctatacc actttgtatg
     2641 cgcacctttc ggggatcgcg cagggcatgc gtcagggaag acgtgtcatc cagggtgaaa
     2701 tcgtcggctt cgtcggcagc accggactgg ccaccggtcc tcacctgcac tacgagtttc
     2761 ttgtcaacgg cgtgcaccac aacccgatgc ggctggcgat gcctccgggc ccgccgatta
     2821 ccgcgcaact gcgttccgag tttgaggaag cggcccggcc gctgttggcg cggctcgata
     2881 tgctgcgcga caccaatctc gcccgcctgg actgaagcgc gcaccattcc agctgcagcc
     2941 cgcggccttt tgccctgata gactcggcaa tcatggccgg taacgaacgt ttcatcgggt
     3001 tgatgtccgg aacgagtctg gacggagtcg acgccgtcct gatggatttc tcagggaacg
     3061 caaaccgggt cgtcggcaat acgttcatcc cttacgacga ccgtctgcgc gcccaacttc
     3121 ttgccctgca ccaaccacat accggggagc tgcataacgc ggccgttctt ggaaatcagt
     3181 tggcgcggat gtacgcgcaa gccgtcgacg agttgcttcg caagaccaag gtcgtccgac
     3241 aagacatcgt cgcgatcggt tgccacgggc agacgatccg gcatcgtccc gatgccggct
     3301 ataccataca actgggcaac ggcgcgctgt tggccgaact cagcggcatt acggtcgtgt
     3361 gcgattttcg cggtcgcgac atcgccgccg gtggtgaagg cgcgccgctg gtgccggcat
     3421 ttcacactgt agtattttcc catctgtcgg tacatcgcgt cgtcgtcaac attggtggca
     3481 tagccaacct gagcgatctt ccaccttcag gagacgtgcg cggcttcgac acagggccgg
     3541 gcaacgcact gatggacgcc tggatccagc gccacctcgg caaacccttc gacaaggacg
     3601 gcgcttggtc gcgaagcgga ctccccgacg acaagctgct gaacgcgctg ctggcgcacg
     3661 aattcttttc gcgcaagccg cccaagagca cggggcgcga aaccttccat ctgcaatggg
     3721 tagaacagta tttgaccggg cgcgaaaagc ccgaagacgt tcaggcgacg ctgctagcgc
     3781 ttacggcgac cagcatcgcc agtagtgtcc ggtcgtactg tgccggcacg agggaaatct
     3841 atctttgcgg tggtggagcg caaaatggtg cactgattgc catcctggaa gcgatgttgc
     3901 cgggagtgct catcgcgcgt accgacgaac tcggtataga cgccgattgg gtggaagcgt
     3961 gtgcgtttgc atggctcgcg cgtcaaacgc tgcagcgacg gccggggaac cttcccgggg
     4021 tcaccggtgc cagagggccg cggattcttg gcgcgatcta tccgaactga agaaaaaagg
     4081 cggcatcaca tgccgccctt gttctgaagg aagcggtggc tacgcggaga aggaagaacc
     4141 gcagccgcag gttgacgttg cgttcggatt cttgatcacg aattgcgcgc cctcgatgcc
     4201 ttcttgatag tcgatctctg cgcctgccag gtactggaag ctcatcgcgt cgatcaagag
     4261 catcacaccg ttcttttcca tcgacgtgtc gtcttcattg acggcttcgt cgaaggtgaa
     4321 tccatactga aagcccgaac agccgccgcc ggttacgaaa acccggagtt tgaggtcggg
     4381 attgccttcc tcgtcgatca gctccttgac cttggtggcc gcgctgtcgg tgaagaccaa
     4441 cggcgacggc atctctgtca ctgcattcat tgcgcgctcc tactgacgaa ttccacaaaa
     4501 accattatct ggctgcgcca ccccggggtc aatcgccgct agaccccttc aacgacacga
     4561 ctttctgtgc cttgtcgtcg tcctggtgca ccaacctgcc ctgcaccacc gctcctagct
     4621 gaatttccag tgtcttgtag tgcacgtctc ctgtgacatt cgattttggc tgcagttcga
     4681 cgtattcggc gccatgcacc gggccgatga cggttccatt gatcacagcg tgggagacgc
     4741 ggatctcccc ctcaatttgc gctttttcgc tcaaaaccaa agtactgggc tgatcatccg
     4801 tggcggcgac gttgccgcgc acacgtccat ccacccgcag gccgccagta aaggaaatat
     4861 tgccctcgac gacagtaccg gtgccgatca ggctgtcgat ccgattctgc ggtttggtgg
     4921 gtttcgctcc gaacattgat gtaccccgct cacgataagg cgacggtctg cgtggatacc
     4981 ggcgtcggat tgccgttctc caacacgcgg acttgcacct tttttacgac agcgccgggt
     5041 gcaaccacga acgtcccctc gacgcgttga tagaacttga agaccagatt atacgcctgc
     5101 gccgactcgc cctcgcgagg caggctgatc acttccgctt tgccgccatc ttcgaggtcg
     5161 acgatgaact ggatatgccc gcggaattcc cgcacccgct gtttcgattg cactatcagc
     5221 agccgatagc gatactcacc cggcagggca tccggctgga cgcgaaagcg gttgacgctg
     5281 acgccgcccg gctcacttcc ggatgccatc agagtctgga aaaacgccag gtcttccttc
     5341 agcagcgcat tctcttcggc aagcgtgtgg atctgagcgg acaagttgcc atgggtcgcc
     5401 agttcgatct gcaactgccg ttctgccgcc gcgatctcgc gccgcaggga ggcattctca
     5461 tccttcaggt tcgaattgag atcctcgagc cgtgcttttt cgcgctgcgc ctcgccctga
     5521 tcgaagcccg catattgccg gccgaaatcg taggtcagcc aggcaacgcc gagcgccagc
     5581 cccgagacga cgatgatcga tagccagcgc caataccacg ggacgtgggt gtgaatcgcc
     5641 acccggggcg cggcaatgcc gaagcggcgc ttgatggttc gtatcagcgt cacggcagca
     5701 aagccacgtg ttcgattccg gtggcctcgg acataccgaa catcaggttc atgttctgca
     5761 ccgcctggcc ggccgcgcct ttcaccagat tgtcgatcac cgacagcacc acgacggtat
     5821 tgccgccacc aggccgatga acggcaatgc ggcagacgtt cgcgcccctg actgaacgcg
     5881 tgtccgggtg gctgccgggt ggcaacacat cgacaaacgg ctcagctgcg tagcgccttt
     5941 cgtaaagcgc ctggacgtcg acctccttgg ttaaacgtcc gtacagcgtg gcatgaattc
     6001 cacggattgc cggggtcaga tgcggtacaa aagtcaggcc gacttttttg ccggcagcac
     6061 ggctgaggcc ttgctcgatt tccggcaagt gacgatgccc gggtaccctg taagccttga
     6121 aattgtcgga tgcttcggag aacaggatgt ggacctccgc tttcttgccc gcgccgctga
     6181 cacctgattt tgcatcagcg atcaaatatt cgatgtccac cacgcccgcc tcgaccagcg
     6241 gaagaaaacc aagctgaacc gaagtgggat aacagcccgg attggcaacc acgcgtgcct
     6301 ttttgatcag atcacggttc acctcaggga gcccgtatac tgcttcggcc acgacctccg
     6361 ggcaggcgtg tgtcatgccg taccactttt gccattcggc gatgtccttg atgcgaaaat
     6421 ccgcggcgat gtcgatcacg cgtacgccgg ctttgagcaa tgccggcgcc tgctccatcg
     6481 ctacgccgtt cggcgtggcg aagaacacga cgtcgcattt ctccagttgt gccgtcgccg
     6541 gatctgagaa tttcagcgac acccgcttcc gcaggctcgg aaacaagtcc gcgaccagca
     6601 tgcccgcttc tttccgcgag gtaatggcgg cgagctccac tccggggtgg gtcgccagaa
     6661 ttcgcagcaa ttcaacaccg gtatagccgg tgccgcctac aatgcctact tttatcattt
     6721 tctccaagga cttatatcat gcttcgataa tatcgcctta cccgatgccg gtgcaaataa
     6781 aaaagccgcc ctgcggcggc tttttcaatg atgccaatgc tcaacgcttc gagaactgct
     6841 tgcggcggcg cgccttgtgc aggcccactt ttttccgctc gacctctcgc gcatcccggg
     6901 tgatcaatcc cgccttgctg agcaccggct tgagcgacgc gtcgtaatcg accagcgctc
     6961 gggcgatgcc gtgccggacc gcgcccgcct ggcccgattc gccgccgccg tcaacattga
     7021 tgaggatgtc gaacgtgttc aggtgattgg tcagttgcag cggctggcgc accaccatgc
     7081 ggccggtttc acgggagaag aactcgtcca ccggcttgtc attgacgacg atgctgcctt
     7141 tgcccggctt gatgaatacg cgcgcgaccg cactcttgcg gcggccggtg ccatagttgt
     7201 atggggtcgt tgccatggat cgtcctagaa attcagcgct tgcggctgtt gcgcggcgtg
     7261 cgggtgctcg ccatcgggat agaccttcag ttttttcagc atcgcatagc caagcgggcc
     7321 tttgggcagc atgcctttta ccgccttttc cagcacgcgg ccggggaatc tgctatgcag
     7381 tttggcgaag ctggtgctgt aaataccgcc cgggtaagtc gagtggcgat agtaaatttt
     7441 ctgttcggcc ttgttgccgg tcacgcgcag tttcttggcg ttgacgacaa caatgtaatc
     7501 accggtgtcc acatgcggag tgaatgtaac tttgtgcttg ccgcgcaggc gtttcgcgac
     7561 ctcggcggcc agtcgcccca gcactttgtc ggtggcgtcg atcacgaacc aatcgcgctt
     7621 cacctcgtgc gtcttggctg aaaatgtttt catggtggtc atcctgcggg gcgtcactta
     7681 gcgaaagccg cgtagtgtag tggaacggcg cgccccttgt caaaccggac tctttggccc
     7741 aagctgcgga tttgtaagcc ttaatcggcc aatctcgcga agcattcagg ttttgcgtgg
     7801 gactaattcc agcaactctt ggagcgggat gcggcacagc cggggacgct tctacggcaa
     7861 gaccatttgg tgaataatgc gtggacgccc gcgcccgccg ttacgttcct tcacgcccca
     7921 ggtttgcttt catgcgcaag ttttggctca tattctccca ggcagtgacg atcagcctgg
     7981 cggcattgtt cgtcgtcgtc accctgcggc ccgatctggt ctcgtggagc gcccgcggcg
     8041 gtggcatcgt caccgtccgc gaggcggcag acggatcggg aaatcgtgcc aacatgactt
     8101 cgctcagtcc ggccgcgaag aaggccatgc cttcggtggt gaacatcttc accagcaagg
     8161 aagtgaaggt tccgcggcat ccgttcatgg acgatccttt attccggcgc ttcttcggtg
     8221 atcagctgga cggcgacacc cagcacagct ccagtcttgg ttccggcgtg atcgtcggcc
     8281 ccgaaggcta tattctcacc aaccatcacg tgatcgaggc ggcagacgag atcgagttgg
     8341 cgctggctga cggccgcaag gcgccggccc gaatcgtcgg caccgatccc gaaaccgacc
     8401 ttgcggtgct caaagtcgac atcgccgacc ttccagccgt cacattcggg aatcctcaaa
     8461 cgctttcggt cggcgacatg gtgctcgcaa tcggcaaccc cttcggggtg ggccagacag
     8521 tgaccatggg tattgtcagc gcgctcggcc gcagccacct gggcatcagc accttcgaga
     8581 atttcattca aaccgatgct gccatcaatc ctggcaattc aggtggcgcg ctggtcgatt
     8641 cggcgggtaa tctgatcggt atcaacagcg cgatctattc gcgcagcggc ggttcgctcg
     8701 ggatcggctt cgccattccg gcggatctcg ccaaacaggt catgcagcag atcgtcggaa
     8761 ccggatcggt gacgcgcggc tggatcggcg tggaggtcca ggatatttca gcggagctcg
     8821 ccgagtcgtt ccggctttcc ggcaccaagg gcacgctcat cgccggcgtg ctgcgcggcg
     8881 ggcccgctga tcgtgccggc gtcaagcccg gagacgttct ggtcgcggtg gaatctaacc
     8941 cggttgtcga ctcgaccaac atgctcaatc ttatcgccgc cctggaaccg ggaaaacagg
     9001 cgacgctgaa agtcctgcgc aaccgcgccg agatgctgat tgaggtaaat gtcggcaagc
     9061 ggccgaagcc cagccgccgt cacggatccg aataatagcc ccggtatctc cgcgaccggt
     9121 caggctgcgg gatcggatct ggtttccgcg gcagcggatt tcccaaccca ctgcgccacg
     9181 aaaatcccga gttcgtacag cacccacaaa ggcacggcaa gcatgacctg cgacaccatg
     9241 tcgggcggcg tgaaaatggc accgatgacg aatgcaccga cgatgacgta gggacggata
     9301 tcgcgcagtt ttgcaacgct taccatcccc atccgtacca ggatcaccac cgccaccgga
     9361 acttcgaagg tcacaccgaa ggccacaaac agggtgagaa cgaagtccag gtacttgccg
     9421 atgtcggtca ttaccgccac accttccggc gcgaccgaga tgatgaagtg aaatacgatc
     9481 ggaaaaacta gaaaatacgc aaacgccatc ccgcacagaa acagcaccgt gctggtaata
     9541 atcaagggca tcaccagttt cttttcatgg gcgtaaagcc cgggcgccac gaaagcccag
     9601 atctgataga gcaggtaggg cagcgcgatc accagcgccg ccatcatcgt caccttgatc
     9661 ggcacaaaaa aaggcgccgt aacttcagtc gcgatcagtt gtccgcccgc gggcaaagac
     9721 gcaagcaagg gttttgcaag catggagtag atattgcgcg cccacggaaa caggcaaatg
     9781 aacaccagca caagcgcgat cacgcaccgt aacagccggt ccctgagttc gaccaggtgc
     9841 gacatgaagg aatcgggctc gatc
//
LOCUS       DASQOP010000093         3423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1844967, whole genome shotgun sequence.
ACCESSION   DASQOP010000093 DASQOP010000000
VERSION     DASQOP010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1844967"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /gene="recR"
                     /locus_tag="VE030_03680"
     CDS             <1..592
                     /gene="recR"
                     /locus_tag="VE030_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HZF22550.1"
                     /translation="TPSSLEELIEALRCLPGVGPKSAQRMAFHLLQRDHPGAQRLGKA
                     LEQALSRVRHCEKCNNFTEQAVCELCSSPRRDQTLLCVVETPADLLMMEQAQAYNGQY
                     FVLMGRLSPLDGIGPKDIHLDRLVKRALDGTVKETILATNFTVEGEATAHYAGELLAT
                     RGMKVTRIARGLPVGGELEHVDSGTLAQAVIERRPA"
     gene            592..1572
                     /locus_tag="VE030_03685"
     CDS             592..1572
                     /locus_tag="VE030_03685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HZF22551.1"
                     /translation="MAGPSAEPAKRRPSSRHSPKSTPAVARGSRRNSGTRLKRMPEPA
                     TPAAPVVARSQKLQKVLAQAGLGSRRAMEEIIKAGKVKVNGTPATLGARVTTDDLIQV
                     GRRQIKFKVTTRLPRVILYHKPEGEIVTRDDPEGRPSVFEKLPAIRSAKWLAVGRLDF
                     NTGGLLIFTTSGELANRLMHPRFEVEREYAVRLYGALTPSQVAEFKKGVRLSDGDARF
                     DLLEEQGGEGRNRWYRVVLKEGRNRVVRRMFEALGLQVSRLMRVRFGIVSLPPRLMRG
                     AWVELKESEISQLLEWAGSTPAAQQDDFSAPVARPSAGTQSRHPRKSPAR"
     gene            complement(1502..2395)
                     /gene="prmB"
                     /locus_tag="VE030_03690"
     CDS             complement(1502..2395)
                     /gene="prmB"
                     /locus_tag="VE030_03690"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HZF22552.1"
                     /translation="MPAEVAKQPRTLRDLLRFAVNRFNAAGLSFGHGTTNARDEAAYL
                     ILHTLKLPLDRLDPFLDKTLAAEEIEAVSRVLRLRIEQRVPAAYLTNEAWLGDFRFYV
                     DERVIVPRSYIAELLRDELSPWVRDPDSVGAALDLCTGSGCLAILLAHAFPNARVDAT
                     ELSPDALEVAKKNVGNYGLQDRVRLVGGDLFAGLIGPYQLIVSNPPYVNADSMAELPP
                     EYRAEPQMALAGGQDGLDIVRHIVSGTGALLDPDGLLVVEIGHNREALEQAFPRLAFT
                     WLETSGGDGFVFLLTGAQLAH"
     gene            complement(2385..>3423)
                     /gene="dapE"
                     /locus_tag="VE030_03695"
     CDS             complement(2385..>3423)
                     /gene="dapE"
                     /locus_tag="VE030_03695"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006395965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HZF22553.1"
                     /translation="ARLGFKAEPMRHGEVDNLWMRRGSRAPLVCFAGHSDVVPTGPLD
                     QWRSDPFTPTVRDGVLYGRGAADMKSSLAAFVTAIEGYLSEHSDHPGSIALLITSDEE
                     GVAVNGTVKVVEALRARAERIDYCIVGEPSSARTLGDTIKNGRRGSLSGELLVRGVQG
                     HIAYPLQSRNPIHQFAPALAELAATEWDPGNEDFPPTTWQVSNIGAGTGASNVIPGEL
                     KASFNFRFSTASTVESLKSRVQAILDRHGLDYALDWSLSGKPFLTARGRLVETLTRAI
                     ERVTGQRPQLSTAGGTSDGRFIAEICPEVVELGPVNESIHKLNEHIAIADLDTLSKIY
                     QQALSDLLNAR"
BASE COUNT          619 a         1096 c         1134 g          574 t
ORIGIN      
        1 aacgccatcc agtctggaag agctgatcga agcgttgcgc tgtctgcccg gcgtcggccc
       61 gaagtcggcg cagcgcatgg ccttccatct tctgcagcgc gaccatccgg gcgcgcagcg
      121 gctcggcaag gcgctcgagc aggcgctttc cagggtgcgc cactgcgaga agtgcaacaa
      181 cttcaccgag caggccgtct gtgagctgtg cagttcgccg cggcgcgatc agacgctcct
      241 gtgcgtggtg gaaacacccg cggacctgct gatgatggaa caggcgcagg cgtacaacgg
      301 ccagtatttc gtcctgatgg gcaggctctc gccgctggac ggcatcggcc cgaaggacat
      361 tcatctcgac cgactggtga aacgcgcgct cgacggcacg gtgaaggaga ccatacttgc
      421 caccaatttc accgtcgagg gagaagccac ggcgcattac gccggagagc tgctggcgac
      481 tcgcggaatg aaggtcactc gcatcgcccg cgggcttccg gtaggcggtg agctcgaaca
      541 cgtggacagc ggcaccttgg cccaggcggt gatcgagcgg aggcctgcct gatggctggt
      601 ccttccgccg agccggccaa gcgccggccc tcgtccaggc actcgcccaa gagcacgccg
      661 gcggtggccc gcggctcgcg cagaaattcc ggaacgaggt tgaagcgcat gcccgagccg
      721 gccacgccgg cggcaccggt cgtagcacgt tcgcagaaac tgcagaaggt gctggcgcag
      781 gccgggctgg gttcgcggcg ggcgatggaa gaaatcatca aggccggaaa agtgaaagtg
      841 aacggcacgc cggccaccct gggcgcgcgc gtgaccaccg acgatctgat ccaggtaggg
      901 cgccgccaga tcaagttcaa agtgaccacg cgcctgccgc gcgtcatcct ctaccacaag
      961 ccggaaggtg aaatcgtcac gcgcgatgac cctgagggcc ggcccagcgt gttcgaaaaa
     1021 cttccggcca tccgcagcgc caagtggctg gcggtcggcc ggctcgactt caacaccgga
     1081 ggtttgctga tcttcacgac ttcgggagag ctggcgaacc gtctgatgca tccgcgcttc
     1141 gaggtcgagc gcgagtacgc ggtcaggctg tacggcgcgc tgacgccatc ccaggttgcc
     1201 gaattcaaga agggcgtgcg gctttccgac ggcgatgcca ggttcgactt gctcgaagag
     1261 cagggtggcg aaggcaggaa ccgctggtat cgcgtggtcc tcaaggaggg gcgcaaccgg
     1321 gtggtgagac ggatgttcga agcgctgggc ctgcaggtca gccgcctgat gcgcgtgcgc
     1381 ttcggcatcg tcagccttcc gccgcgcctg atgcgcggcg cctgggtcga actgaaggag
     1441 tccgagatca gccagttgct cgagtgggcg ggcagtaccc ccgccgcaca gcaggacgat
     1501 ttcagtgcgc cagttgcgcg cccgtcagca ggaacacaaa gccgtcaccc ccggaagtct
     1561 ccagccaggt gaacgctagc cggggaaacg cctgctccag ggcctcccgg ttgtgcccta
     1621 tttccaccac cagcaggccg tccggatcca ggagcgcacc cgtgcctgac acgatgtggc
     1681 gcacgatatc cagtccatcc tgcccacccg ccagcgccat ttgcggttcg gcacgatact
     1741 caggcggcaa ttccgccatc gaatccgcat tcacatacgg tggattggaa acgatgagct
     1801 ggtatggccc aatgagaccc gcgaacagat cgcctccaac gaggcgaacg cggtcctgca
     1861 ggccgtaatt cccgacgttt ttctttgcga cctcgagcgc atcgggcgag agttcggttg
     1921 cgtccacccg ggcgttgggg aaagcatgcg ccagcaggat cgccaggcag cctgagccgg
     1981 tacacaggtc cagcgctgcg ccgactgaat ccgggtccct cacccaggga ctcaactcgt
     2041 cccgcagcaa ctccgcgata tatgagcgtg gcacgatcac gcgctcgtcg acataaaaac
     2101 ggaaatcgcc cagccaggct tcgttcgtta ggtaggccgc cggcacgcgc tgttcgatgc
     2161 gcaggcgcag cacccggctc accgcctcga tttcttctgc ggccagcgtc ttgtcgagaa
     2221 acggatcgag gcgatcgagc ggaagtttca gcgtgtgcag gatcaggtac gccgcctcgt
     2281 cgcgggcatt ggtggtgccg tgaccgaacg acaacccggc tgcgttgaag cggttcaccg
     2341 cgaagcgcag caagtcccgc aaggtacgcg gctgcttcgc cacctcagcg ggcattcagc
     2401 agatcgctca gcgcctgctg gtatatcttc gacaacgtat ccagatcggc gatggcaatg
     2461 tgctcattca atttgtggat cgactcgttc accgggccca attcaacgac ctcggggcag
     2521 atctcggcga tgaagcgtcc gtccgacgtg ccgccggctg tagataattg cggccgctgg
     2581 ccggtgacgc gctcgatcgc gcgggtcagc gtttccacca gcctgccgcg ggcggtcagg
     2641 aagggtttgc cggatagcga ccagtccagt gcgtagtcca gaccgtggcg gtcgagaatg
     2701 gcttgcacgc ggctcttcag cgactcgacg gtgctggcgg tggagaagcg aaagttgaac
     2761 gacgccttca gttctcccgg aatcacgttg ctggcgccgg tgccggcgcc gatgttcgac
     2821 acttgccagg tggtcggtgg aaagtcttcg ttgcccgggt cccattcggt ggcggcgagt
     2881 tcggccagcg ccggcgcgaa ctggtggatc ggattgcgcg actgcagggg gtaggcgatg
     2941 tggccctgga cgccgcgcac cagcagttcc ccggacagcg atccgcggcg gccgttcttt
     3001 atggtgtcgc cgagcgtcct ggccgacgaa ggctcgccga cgatgcagta gtcgattctt
     3061 tcagcccggg cgcgcagcgc ctcgactacc ttcaccgtgc cattgaccgc cacgccttcc
     3121 tcgtcggagg taatcagcag cgcaatggag ccgggatggt ccgaatgttc ggagagatat
     3181 ccctcgattg cggtgacgaa cgctgccagc gaggacttca tgtcggctgc gccgcggccg
     3241 tagagcacgc cgtcgcgcac cgtcggagtg aacggatcgg atcgccactg gtccagcggc
     3301 ccggtcggga ccacgtcgct gtgtcccgcg aagcagacga gcggcgcgcg gctgccgcgc
     3361 cgcatccaga gattgtcgac ttcgccgtgg cgcatcggct ccgccttgaa gcctagccgc
     3421 gcc
//
LOCUS       DASQOP010000094         3426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_36293, whole genome shotgun sequence.
ACCESSION   DASQOP010000094 DASQOP010000000
VERSION     DASQOP010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36293"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VE030_03700"
     CDS             <1..330
                     /locus_tag="VE030_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HZF22554.1"
                     /translation="IESAGSASAFRNTTEAVRPGGQVIWLGKIDVNKDVSFRWGSLMQ
                     EKRIRRVSYGNARPRRDFPLLARAYLDGALMLDELISRRITLDGINDGFDALKRGEAI
                     RSVITFD"
     gene            complement(413..1099)
                     /locus_tag="VE030_03705"
     CDS             complement(413..1099)
                     /locus_tag="VE030_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZF22555.1"
                     /translation="MKSKTSTKRRSRAIEVEDGTLTDRAYRVLEEMIVTLQLSPGTVL
                     SEQALAVRLKIGRTPIREALQRLARDGLVVIMPRRGIMVSEINLRLQLRLLEVRRELE
                     RLMASLAAERATPEERREFAEVAEAMLTAAAKADDIAFMRLDQRFNILIATAARNEFA
                     RRSMGLMNALSRRFWYQHYQQVADLPLAAKLHAAVAEAVAQKKAKAAATASDRLIDYI
                     EDFARKTLDT"
     gene            complement(1490..2500)
                     /locus_tag="VE030_03710"
     CDS             complement(1490..2500)
                     /locus_tag="VE030_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZF22556.1"
                     /translation="MPRLILGAFAAASLGLLISGSAHAAKPFPSPSAPAPVFYRSVKV
                     DGLDIFYREAGAANAPTLLLLHGFPSSSRMFEPLLARLSGQFHLLAPDYPGFGHSDAP
                     DPKAFAYTFDHIAEVMQHFTEAVGSSRYVLYMQDYGGPVGFRLAVAHPERVQGIIIQN
                     AVAHDDGLGPRWNRMREFWADRAANEAGFRQSLTSLAGAQRRHLGASPNATNYDPDLW
                     SDEFAFLNKSGESDIQIDLFFDYRTNVASYSAWQAWLRQAKPPTLVVWGRYDQSFEAA
                     EAQAYKREIPDADVHLIDAGHFALDEKPDEVAALIGAFMSKLSKSRRPRSTPMKSAYT
                     PR"
     gene            complement(2572..2826)
                     /locus_tag="VE030_03715"
     CDS             complement(2572..2826)
                     /locus_tag="VE030_03715"
                     /inference="COORDINATES: protein motif:HMM:NF018294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HZF22557.1"
                     /translation="MTMHRDIRSFRPAAPTIRRRLTVLLNRWVAAMIAHREREAARFV
                     LGHLNDRALKDIGIYRSQIDDGIAEIARRRARLQRSERPR"
BASE COUNT          669 a         1013 c         1027 g          717 t
ORIGIN      
        1 atcgagtcgg ccggcagcgc gtcggccttt cgcaacacca ccgaagccgt gcggcccggc
       61 ggccaggtca tctggctcgg caagatcgat gtgaacaagg acgtctcgtt ccgctggggc
      121 tcgctgatgc aggagaaacg catccgccgc gtcagttacg gtaacgcacg cccgcgccgc
      181 gattttccgc tgctggcgcg ggcctatctc gatggcgccc tgatgctcga cgagttgatc
      241 tcccgccgca tcacgcttga cgggatcaac gacggcttcg atgcgttgaa gcgcggtgaa
      301 gccattcgca gcgtcattac atttgactga tgcgcttgga gcccagaggc tctatgcttt
      361 atttgacgcg ttttcttcac gcgaaccgga ttccacttcg ctcgaaaacg cttcatgtat
      421 cgagcgtctt gcgcgcgaaa tcctcgatgt agtcgatcag ccggtccgac gcggtcgcgg
      481 cggctttcgc cttcttctgc gcgaccgcct ccgcgaccgc ggcgtgcagc ttggcggcga
      541 gcggcagatc cgcgacctgc tgatagtgct gataccagaa ccggcgcgac aacgcgttca
      601 tcaggcccat cgatcgccgg gcgaattcgt ttcgggccgc tgtcgcgatc aggatgttga
      661 agcgctggtc gagccgcatg aatgcgatgt cgtcggcctt tgcggccgcg gtgagcatgg
      721 cttctgccac ctccgcgaat tcgcggcgtt cctccggcgt ggcgcgctcg gccgccaggc
      781 tcgccatcag gcgctccagc tcgcgccgca cctcgagcag ccgcagctgc agcctcaaat
      841 tgatctcgga gaccatgatg ccgcgcctgg gcatgatcac gaccaggccg tctctcgcaa
      901 gccgctgcag ggcttcccgg atcggggttc ggccgatctt cagacgcacc gcaagcgcct
      961 gctcggacaa caccgttccg ggtgataatt gcagcgtgac aatcatctct tcaagtacgc
     1021 gataggcacg atctgtcagc gtaccgtcct cgacctcgat ggctcgagat cggcgcttcg
     1081 tcgatgtttt cgatttcaaa tcgtccctca catcaccccg gcgagagcgc cggcgggtcg
     1141 ggtgctgcac actcatccca agcgtatatt ggccatatat catgacgcaa caccgcagag
     1201 cgatcgcagc gtttgccgac ggaaatgcgc taatcggctg gccgagatca agcaaggcgg
     1261 ggggccgcgc gttcatctcc gcagtcctcc tttcgtctcg ggcagtgagt aattattatg
     1321 atatttcaat gttttatagg gggtgcagca ggcaggaccg gccgctttgc caaccggtcc
     1381 ggcgcccaga aattctgccc ttggcgatcc gtgaaatgct tgaaagctcc agggcggacg
     1441 gcggatcgga gatcgttgag agccgagtac cgccatatgc caacagcggt tatcgcggcg
     1501 tatacgcgct cttcattggc gtcgatcgcg ggcgccttga ctttgaaagc ttggacatga
     1561 acgcgcctat gagggccgcc acctcatcag gcttctcgtc gagcgcaaaa tggccggcgt
     1621 cgatcaggtg aacgtcagcg tcgggaatct cgcgtttgta agcttgcgct tccgccgcct
     1681 caaacgattg atcgtatcgt ccccacacca cgagcgtcgg cggcttcgct tgcctgagcc
     1741 aggcctgcca cgcggaataa cttgcgacgt tggtccggta gtcgaagaag agatcgatct
     1801 ggatgtcgct ttcgccagat ttgttgagaa acgcgaactc gtccgaccac aggtccggat
     1861 cataattcgt ggcgttggga ctggcgccga gatgcctcct ttgcgctccg gccagggacg
     1921 taaggctttg tctgaagcct gcctcattcg ccgctcggtc cgcccagaat tcccgcattc
     1981 ggttccaacg cggtcccaaa ccgtcatcgt gggcgacggc gttctggatg ataatgccct
     2041 gcacgcgttc aggatgcgcg acggcgagtc gaaatccaac aggaccacca taatcctgca
     2101 tgtacagtac atagcggctc gacccgaccg cttccgtaaa gtgctgcatg acttccgcga
     2161 tatgatcgaa ggtataggcg aatgctttag gatctggcgc gtcgctatgt ccgaagcccg
     2221 gatagtctgg ggctaacaaa tggaattgcc cggaaagccg cgccaacaga ggctcgaaca
     2281 tccgtgagga cgagggaaag ccgtgcagga gcaggagtgt cggcgcattc gccgctccgg
     2341 cttcgcgata gaaaatatcg aggccatcga ctttgacgga acgatagaaa accggcgctg
     2401 gagcgctggg agaagggaag ggtttggctg cgtgtgccga tccggaaatc agcagtccga
     2461 gactggcggc ggcgaaggct cccaagatca atctaggcat gcatcctctg agttataatt
     2521 gtgtctcgtg tcctgctggt ggtgaccagt agcggatcga ggggttcgcg tctatcgagg
     2581 ccgctcggat cgttgcagcc gcgcgcgtct tcgcgcgatc tccgcgatac cgtcgtcgat
     2641 ctgagatctg tagatgccga tgtcttttag cgcccggtcg ttgagatgac ccagcacgaa
     2701 acgtgccgcc tcgcgttcgc gatgcgcgat catagcggcg acccagcgat tgaggaggac
     2761 agtgagccgt ctccggatgg tcggcgcggc cggtcggaat gatcggatat ccctgtgcat
     2821 cgtcattgtc gttttcctct atctgacgtc tcacgctgcg cgcgtgacgg cgaaaaaaat
     2881 gagccgtcac gcgcgcgtga agcgtgaaga agcgaaacaa gggctgtcgc tccacgatgt
     2941 ccgtatgaat ggtttgacga ttcgcggcgt catccggacg ggggacgcag attgcatatc
     3001 gactgttccg caccgatgcg ggcgatgtta ttcgacgccg ttggaataat ttagaatcga
     3061 tcaaagacag cgcggaatcc gatgatgagt ccccatcacg atttgatgat cgcgacatcg
     3121 accgttcggt ttcgcctggc ctgaaacggc tgcgctctca ccagaacgcc ctgcgagcgc
     3181 gtgaggccat caccggcgtt acgtcgcttg agcgaactcc tgcatcgatt ttgtacaata
     3241 caatcccatg tgccaataag atgctcgcga tcacacctat tagaaaaaga cgattcagca
     3301 tgaccgtgta tccgaatact caattcagct cgcgcattgc gaacgggtga agcgacctgg
     3361 ctgatggatg cgcgcatcga cctttgcctc cagtgcgccg atacataggt tttggatcgc
     3421 gattcg
//
LOCUS       DASQOP010000095         2476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2048783, whole genome shotgun sequence.
ACCESSION   DASQOP010000095 DASQOP010000000
VERSION     DASQOP010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2048783"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..95
                     /locus_tag="VE030_03720"
     CDS             <1..95
                     /locus_tag="VE030_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HZF22558.1"
                     /translation="TGKIGDGKIFVFDLKQVIRIRTGETGAEAL"
     gene            108..1613
                     /gene="amt"
                     /locus_tag="VE030_03725"
     CDS             108..1613
                     /gene="amt"
                     /locus_tag="VE030_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HZF22559.1"
                     /translation="MKKLFAILALLGAVGWCAPVMAQDKPAAPAVAPAAPAPVAAAPA
                     AAPAAAPSATPAPAPTPNKGDVAWMLTSTALVLMMSVPALALFYGGLVRSKNMLSTLM
                     QVFVGFSLITVLWCIYGYSLAFTEGNSFFGSLTERLFLKGTFDPVKGEFANAATFSKN
                     TPLPELVYVAFQATFAAITCCLILGATVERIKFSAVLIFLALWFTFSYTPVAHMVWFF
                     PGPDAFTDPKTVDAVTAKAGLIWQWGALDFAGGTVVHINSGVAGLVGAYLVGKRIGIG
                     KEAMAPHNVTMTMIGAALLWTGWFGFNAGSALEANGSAALAFMNTYLATACAVLSWMF
                     GEWLFKGKPSMVGAASGAVAGLVAITPAAGNVGIPGAFVIGTAAGLVCLWGVNGLKKM
                     LGADDALDVFGVHAVGGILGALLTGVFNSPDLGGPGSVQDWVTMKSGYPGIMAQLWIQ
                     AKAVGLVFVWTAVVAFISFKIADLLVGLRVTEEEEREGLDINSHGETAYRI"
     gene            1839..>2476
                     /locus_tag="VE030_03730"
     CDS             1839..>2476
                     /locus_tag="VE030_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008164308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZF22560.1"
                     /translation="MKMEYVISSWDIPSLPIVGSNARFPVRRIFCVGRNYVEHQKEMG
                     GDGREQPFFFAKSAHDLVPEGGAVNYPPMTSNYHWETEMVAVIGTGGYKIPAAKANDH
                     IWGYAVGLDMTRRDLQQVGKDKGRPWTFGKDFDQSAPVGPITPAAKSGHPKKGKLWLK
                     VNGELRQNSDIDQMIWSVPEQIAFLTQYYTLEAGDLIMTGTPAGVGAAKPGD"
BASE COUNT          447 a          747 c          786 g          496 t
ORIGIN      
        1 acacgggaaa gatcggcgac ggcaagatct tcgtcttcga tctgaagcaa gtcatccgca
       61 tccgcaccgg cgagaccggg gcggaagcgc tctgagggag aaccacgatg aaaaagcttt
      121 ttgcaattct tgccctgctc ggcgccgtgg gctggtgcgc gccggtcatg gcccaggaca
      181 aaccggcggc tccggctgtt gcacccgccg cgcccgcgcc agttgcggct gctcccgcag
      241 cggctcctgc ggccgccccg agcgccactc cggctccggc gcccacgccc aacaagggcg
      301 acgtcgcgtg gatgctcacc tccaccgcgc tcgtgctcat gatgagcgtt ccggccctgg
      361 cgctgttcta cgggggcttg gtgcgctcga agaacatgct ctcgaccttg atgcaggtgt
      421 tcgtcgggtt ttcgctcatc accgtcctct ggtgcatcta cggctacagc ctcgcgttca
      481 ccgaaggcaa ctcctttttc gggagcctta ccgagcggct gtttctgaaa ggcaccttcg
      541 atccggtgaa gggtgagttc gcgaacgcag cgacgttcag caagaacaca ccgctgcccg
      601 agctggtcta tgtggctttc caggcgacct tcgctgcgat cacttgctgc ctgatcttgg
      661 gagccacggt cgagcggatc aagttttccg cggtcctgat ctttctggcg ctgtggttca
      721 ctttcagcta tacgccggtt gcccatatgg tgtggttctt ccccggtccg gatgccttca
      781 ccgatcctaa aacagtggat gccgtgacgg caaaggctgg gcttatttgg cagtggggcg
      841 cgctcgactt tgccgggggc acggtagtgc acatcaattc cggggtcgcc ggattggtgg
      901 gtgcctatct ggtcggcaag cgcatcggga tcggaaaaga ggcaatggcc ccgcacaacg
      961 tcaccatgac catgattggt gctgcactgc tgtggaccgg ttggttcggg ttcaacgcag
     1021 gatccgcgct ggaagcaaat ggttcggctg cgctcgcgtt catgaacact tacctcgcga
     1081 cggcttgcgc cgtgctttca tggatgttcg gcgagtggct gttcaagggc aagccgagca
     1141 tggtcggcgc ggcctcaggc gcggttgcgg gcctcgtcgc gatcacgccc gccgccggca
     1201 acgtcggtat tccgggtgca ttcgtgatcg gcacggctgc cggcctggtg tgcctgtggg
     1261 gtgtgaacgg tctgaagaaa atgctgggtg cagatgatgc actcgacgtg ttcggcgttc
     1321 acgcagtcgg cgggatactc ggtgcgctgc tcaccggcgt gttcaactcg cctgacttgg
     1381 gtggacccgg ctccgtgcag gactgggtca cgatgaagag cggttatccg ggaatcatgg
     1441 ctcaactctg gatccaagcg aaagcggtgg gcctggtatt cgtctggacc gccgtggtgg
     1501 ccttcatctc cttcaagatc gcggatctcc tcgtgggact ccgcgtcacc gaagaagagg
     1561 agcgcgaggg cctggacatc aactcgcacg gcgagacggc ctatcgcata taactttcag
     1621 gcaaaaccga ttggggggat atacaagggg gcatcgcccc cttgttccca aacggtagca
     1681 gggagaagcc cggaaacggg cttgctttcg aacgggcacc ttcgggtgcc cgtttttctt
     1741 gcgccggcag ttcatgcgaa aatacgcacc tttcgccggc acccggcaat tcggttcgcg
     1801 ccgcacttct cttgcgatcc tgtgaggacc ctctcgtcat gaaaatggaa tacgtcatca
     1861 gcagctggga catcccttcg ctacctatcg tgggctcgaa tgcccgcttt cccgtgcgcc
     1921 gcatcttctg cgtcggccgc aactatgtag agcaccagaa ggagatgggc ggggacggcc
     1981 gcgagcagcc gttctttttc gccaaatcgg ctcatgatct cgtccccgag ggtggcgcag
     2041 tgaactatcc gccgatgacg agcaactacc actgggagac cgagatggtt gcggtgatcg
     2101 gcacgggtgg ctacaaaatc ccggcggcga aagccaacga ccacatctgg ggctacgcgg
     2161 tgggcctgga catgacccgg cgtgatttgc agcaggtcgg aaaggacaaa ggcaggccct
     2221 ggaccttcgg caaggatttc gaccagtccg ccccggttgg gcccattacc cccgccgcaa
     2281 agagcgggca cccgaaaaaa ggcaagctct ggctcaaggt gaacggcgag cttcgccaga
     2341 actcggatat cgaccagatg atttggagcg tgcccgaaca gatcgcgttt ctcacgcagt
     2401 actacaccct ggaagcgggc gatctcatca tgaccggcac gccggccggt gtcggcgccg
     2461 ccaagccggg ggatga
//
LOCUS       DASQOP010000096         7227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1649177, whole genome shotgun sequence.
ACCESSION   DASQOP010000096 DASQOP010000000
VERSION     DASQOP010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7227
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649177"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VE030_03735"
     CDS             complement(<1..291)
                     /locus_tag="VE030_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZF22561.1"
                     /translation="MKGKDEGGRRKDEGEPEAIELTSSFIPHPSAFRVAIVGGGYAGM
                     AAAAELARRDIPVTVFEAARTLGGRARRVEINGMTLDNGLHILIGAYRETLRL"
     gene            complement(294..1127)
                     /gene="hpnD"
                     /locus_tag="VE030_03740"
     CDS             complement(294..1127)
                     /gene="hpnD"
                     /locus_tag="VE030_03740"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HZF22562.1"
                     /translation="MSPDQYCQDKAAQSGSSFYYSFLFLPPEKRRAITALYAYCREVD
                     DVVDECSDADLARAKLAWWRQEITGTFAGTAQHPVAKALIPVVRRFDLPQARFHEIID
                     GMEMDLQYNRYPDFATLQSYCYRVAGVVGLMAAQIFGYRDPATLRYAENLGTAFQLTN
                     IIRDVGEDARRNRVYLPLDELARFGVAASDVAHLRETDSFRRLIEFQIDRARDYYQRA
                     FASLPRQDRKAQRPGLVMAAIYRTVLDEIHAGGCKVLSQRTSLTPLRKLWIAWRTWFT
                     A"
     gene            complement(1124..1939)
                     /gene="hpnC"
                     /locus_tag="VE030_03745"
     CDS             complement(1124..1939)
                     /gene="hpnC"
                     /locus_tag="VE030_03745"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HZF22563.1"
                     /translation="MAVNHYENFPVASVLLPARLRRPVELIYSFARQADDFADEGVLP
                     PEQRLHKLAEFSRELDCVERGEPPRLPLFADLAPVIAAHRLPVGLFRDLLSAFTQDVT
                     KGRYADFREVMDYCRRSANPVGRLLLHLYQATEARNLACSDAICSSLQLINFLQDIEI
                     DFRKNRIYLPQDEMAEYGVTEEQIADRAGGTTWQTLMVFQIERAHSMLLAGAPLARRL
                     PGRIGMELRMIVMGGSRILDKLRASRGDVFRHRPVLQPYDWLAMLFRSLFAYP"
     gene            2045..2129
                     /locus_tag="VE030_03750"
     tRNA            2045..2129
                     /locus_tag="VE030_03750"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2079..2081,aa:Leu,seq:tag)
     gene            2196..3503
                     /gene="tig"
                     /locus_tag="VE030_03755"
     CDS             2196..3503
                     /gene="tig"
                     /locus_tag="VE030_03755"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HZF22564.1"
                     /translation="MQADLETLSSLERRLSVTLPLDQVNSEVENRLKRLARTVKLHGF
                     RPGKVPLKIVEQQFGGQVRQEVLGDAVQSSFGEAVKEKNLRVAGYPRIEVKSAADAEG
                     KFEYTATFEVYPELAIGELGSVKIERPLVKIGDAEADKTIEILRKQRTHYHSTGRAAQ
                     SGDQVLMDYRGSIDGNPFDGGAGTDQHALLGGGQLLADFEQNVLGMKAGETRTFELRF
                     PDDYHGKEVAGKTARFEVTVKDVREPHVPEADAEFAKALGVADGDIAKMRAEIKSNLE
                     REVKRRAGTRVKDQVMKALLDATRVELPRALLDMEIDRLLHNVRHDLEARGLKADKVP
                     MPREAFEPEAQRRVSLGLILAELVKKHGLEAKPDQIKAVVGEYAHSYEKPEEVLRWYY
                     QSPDRLREVESIVLEDNVVQWVLANARVEDKPTEFDDLMGNAK"
     gene            3500..4144
                     /gene="clpP"
                     /locus_tag="VE030_03760"
     CDS             3500..4144
                     /gene="clpP"
                     /locus_tag="VE030_03760"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HZF22565.1"
                     /translation="MITMQDRIMSEPQGLGLIPMVIEQSGRGERAYDIYSRLLKERVV
                     FLVGPVNEMSANLIVAQLLFLESENPEKDISFYINSPGGSVSAGLAIYDTMQFVKPDV
                     STLCIGQAASMGAFLLAAGAKNKRFALPNSRVMIHQPMGGFQGQASDIEIHAREILYL
                     RGRLNELMAKHSGQDVKTIERDTDRDNFLSAEESVKYGLVDKVLVSRAEAGAPA"
     gene            4232..5497
                     /gene="clpX"
                     /locus_tag="VE030_03765"
     CDS             4232..5497
                     /gene="clpX"
                     /locus_tag="VE030_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HZF22566.1"
                     /translation="MSDKPGGEKLLYCSFCGKSQHEVRKLIAGPSVFICDECIELCND
                     IIREESHAETTGKGAKSDLPVPHEIREILDQYVIGQDTAKKILSVAVYNHYKRLKTVQ
                     KKDDVELAKSNILLIGPTGSGKTLLAQTLARLLNVPFVMADATTLTKAGYVGEDVENI
                     IQKLLQKCDYDVEKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGTI
                     ASVPPQGGRKHPNQEFVQVDTTNILFVCGGAFDGLDRIIRARSDKGGIGFGAEVKSKD
                     NRKDIGAVLRGVEPEDLIKYGLIPEFVGRLPVVATLDELDQAALIQILTEPKNALLKQ
                     YQRMFSMEGVELEVRSEALKAVALKALARRTGARGLRSILEQVLLDIMYDLPSLNNVE
                     KVVVDEGMITGDAQPILLYSDQPKVAGSA"
     gene            5641..>7227
                     /gene="lon"
                     /locus_tag="VE030_03770"
     CDS             5641..>7227
                     /gene="lon"
                     /locus_tag="VE030_03770"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017332019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HZF22567.1"
                     /translation="MTYSADSSQTTVTYPLLPLRDVVVFPHMVIPLFVGRPKSIKALE
                     TAMEAGKSILLVAQKSAAKDEPSPEDLYDIGSIANILQMLKLPDGTVKVLVEGVQRAK
                     VVSVADEKTHYAAEALPVPADAVETNEVEAMRRAMLTQFDQYVKLNKKIPPEILTSLS
                     GIDEPGRLADTVAAHLPLKLEQKQAVLEMFEVRKRLEHLLSLMEAELDILQVERRIRG
                     RVKRQMEKSQREYYLNEQVKAIQKELGDMEEGADLDEMDKRIKAAHMPKEARNKAEAE
                     LKKLRLMSPMSAEATVVRNYIETLVGLPWKKKSKISNDLAKAQEILDADHYGLDKVKE
                     RIVEYLAVQQRVEKLKAPILCLVGPPGVGKTSLGQSIAHATNRKFVRMSLGGVRDEAE
                     IRGHRRTYIGSMPGKLLQNMSKIGVRNPLFLFDEVDKMGQDFRGDPASALLEVLDPEQ
                     NDKFVDHYVEVEYDLSDVMFVATANTMNIPAPLLDRMEVIRLSGYTEDEKINIAMRYL
                     LPKQMKNNGVKEGELSVSESA"
BASE COUNT         1539 a         2161 c         2305 g         1222 t
ORIGIN      
        1 cagccgcaag gtttcccgat acgcgccgat cagaatatgc aagccgttgt cgagcgtcat
       61 gccgttgatc tcgacccggc gcgcccgccc gccgagcgtc ctggccgcct cgaataccgt
      121 aaccggaatg tcgcggcggg caagctccgc cgccgccgcc attccggcat agccaccacc
      181 aacaatcgct acgcgaaagg ctgaaggatg agggatgaag gatgaagtaa gttcaatagc
      241 ttctggctct ccttcatcct tccgccttcc gccttcatcc ttgcctttca tactcacgca
      301 gtgaaccacg tgcgccacgc gatccacaat ttgcgcagcg gggtgagcga tgtgcgctgg
      361 ctgaggactt tgcatccgcc ggcgtgtatc tcatccagga cggttcggta gatcgccgcc
      421 attaccagac ccgggcgctg ggccttgcgg tcctggcgcg gcaaagaagc gaaggcgcgc
      481 tggtaatagt cccgggcacg gtcgatctgg aattcaatca atcgccggaa ggaatcggtc
      541 tcacgcaaat gagcgacatc ggacgcggca acgccgaagc gcgccagttc gtcgagcggc
      601 agatagaccc gattgcggcg cgcatcctcg ccgacgtcgc ggatgatatt ggtgagctgg
      661 aacgccgtgc cgaggttttc ggcatagcgc agcgttgcgg gatcgcggta gccgaagatc
      721 tgtgccgcca taaggccgac cacgcccgcc acccggtaac aatacgattg cagcgtggcg
      781 aagtcgggat agcggttgta ctgcagatcc atctccatcc cgtcgatgat ctcgtggaag
      841 cgcgcctgcg gtagatcgaa acggcgcacc accgggatca gggcctttgc caccggatgt
      901 tgcgccgtgc cggcaaatgt gccggtgatt tcctggcgcc accaggcgag cttggccctg
      961 gcgagatcgg cgtcgctgca ctcgtccacc acgtcgtcca cctcgcgaca gtaggcgtag
     1021 agcgcggtga tcgcgcggcg tttttccggc ggcaggaaca ggaagctgta gtagaagctc
     1081 gagccgcttt gcgccgcctt gtcctggcaa tactggtcgg ggctcatgga taggcgaaca
     1141 acgatcggaa cagcatggcg agccagtcgt agggttgcaa caccgggcga tgacggaaaa
     1201 cgtcgccgcg gctcgcgcgc agcttgtcca ggatgcggct gccgcccatc acgatcatgc
     1261 gcaattccat tccgatgcgc cccgggagcc gtctcgccag cggcgcgccg gccagcagca
     1321 tgctgtgtgc gcgctcgatc tgaaacacca tcagtgtttg ccatgtcgtg ccgccggcgc
     1381 ggtcggcgat ttgctcttca gttacgccgt attcagccat ctcgtcctga ggcaggtaga
     1441 tgcggttctt gcggaaatcg atctcgatgt cctgcaggaa gttgatcagc tgcaggctcg
     1501 aacagatagc gtcggagcac gcgaggttgc gcgcttcggt ggcctgatac aagtgcagca
     1561 gcagcctgcc gactggattg gccgagcgcc ggcaatagtc catgacttcg cggaaatcgg
     1621 cgtaacggcc cttggtcacg tcctgggtga acgccgaaag caggtcgcga aacagaccga
     1681 cgggcaggcg atgcgccgct atcaccggtg ccagatcggc aaacaacggc aatcgcggcg
     1741 gctccccgcg ttcgacgcaa tccagctcgc gggagaactc ggcaagcttg tgcaaacgct
     1801 gttccggcgg caatacgccc tcgtccgcga aatcgtcggc ctggcgtgca aacgagtaga
     1861 tcagctcgac cggacggcgc agccttgcgg gcagcaacac cgaagccacc ggaaagttct
     1921 cgtagtgatt gaccgccatc ggctattcgc tttgtgtgac agaaggttag acggcggaac
     1981 gagggcccgg caagcgggca tttgggtagc gggggcgggg gcattatatt agaatgcgtc
     2041 gagtgcgaaa gtggcggaat tggtagacgc accagattta gggtctggcg ccgaaaggcg
     2101 tgagagttcg agtctctcct ttcgcaccag agccacgaaa aaagcgctgt ctgccaagtc
     2161 ttacccgcct accaacacag gaaaaccgaa attagatgca agcagatctg gaaactctga
     2221 gctctctcga gcggcgcctc tcggtaacct tgccgctcga tcaagtcaac agtgaagtcg
     2281 agaaccgcct caagcggctt gcacgcaccg taaagctgca cggcttccgt cccggcaagg
     2341 tgccgctcaa gatcgtcgaa cagcagttcg gcggccaggt gcgccaggaa gtgctcggcg
     2401 atgccgtgca aagcagcttc ggcgaggcgg tcaaggaaaa gaacctgcgc gttgccggct
     2461 atccgcgcat tgaagtgaag tccgcggcgg acgccgaagg caaattcgag tacaccgcga
     2521 ccttcgaggt ttacccagaa ctcgccatcg gtgaactggg aagcgtgaaa atcgagcgtc
     2581 cgctcgtcaa gatcggcgat gccgaggcgg acaagaccat agaaatactg cgcaagcagc
     2641 gtacccacta ccacagcacc ggccgcgccg cccagtccgg cgaccaggtg ctgatggatt
     2701 accgcggcag tatcgacggg aaccctttcg acggaggtgc gggaaccgat cagcacgccc
     2761 tgctcggcgg cggccagttg ctcgcggatt tcgagcagaa cgtcctcggc atgaaggctg
     2821 gcgaaaccag gaccttcgag ctgcgctttc ccgatgatta ccacggcaag gaagtggcgg
     2881 gaaagaccgc ccggttcgaa gtaacggtga aggatgtgcg cgagccccac gtccccgaag
     2941 cggatgccga atttgccaag gccttgggcg ttgcggacgg cgacatcgcc aaaatgcgcg
     3001 ccgagatcaa gtccaacctc gagcgcgagg tcaagcgccg tgccggtacg cgcgtcaagg
     3061 accaggtcat gaaagcgctg ctcgacgcga cccgggtgga gctgcccagg gcgttgctgg
     3121 acatggaaat cgatcgcctg ttgcacaacg tgcgccacga tctggaggcg cgcgggctca
     3181 aggcagacaa ggtgccgatg ccgagagagg ccttcgagcc ggaagcgcag cggcgtgtca
     3241 gcctcggcct gatcctggcg gagctggtga agaaacacgg acttgaagcc aagccggacc
     3301 agatcaaggc ggtggtcggg gaatatgcgc atagctacga gaagccggaa gaggtgctgc
     3361 gctggtacta ccagtcgccc gaccggttgc gcgaggtcga gtccatcgtc ctcgaggaca
     3421 acgtagtgca atgggtgctg gccaatgcca gggtcgagga caagccgacc gagttcgacg
     3481 acctcatggg gaatgcaaaa tgatcacgat gcaagaccgg ataatgagcg agccgcaggg
     3541 cctgggattg atacccatgg tcatcgagca gagcggccgc ggcgagcgcg cctacgacat
     3601 ctactcgcgg ctgctcaagg agcgcgtggt gtttctggtc gggccggtca atgaaatgtc
     3661 ggcgaacctg atcgtggcgc agttgctctt tctcgaatcg gagaacccgg aaaaagacat
     3721 ttccttctat atcaactcgc cgggcgggtc ggtttccgcg gggttggcga tttatgacac
     3781 catgcagttc gtcaagcctg acgtgagcac cctttgcatc ggccaggcgg ccagcatggg
     3841 agccttcctg cttgctgccg gcgccaagaa caagcgcttc gcgctgccta actcgcgagt
     3901 catgatccac cagccgatgg gtggcttcca gggccaggct tcggacatcg agatccacgc
     3961 gcgtgaaatc ctctacctgc gcggccggct caacgagctg atggcgaagc acagcggaca
     4021 ggacgtgaag accatcgagc gcgacaccga ccgcgacaat ttcctcagcg ccgaagagtc
     4081 ggtaaagtac gggctggtgg acaaggtact ggtcagccgc gccgaggccg gcgcgcccgc
     4141 ctgaggcgca gggcggatcg cggcgcggcg acacgggaac caacgtgcgt atgggtgtct
     4201 gatgggttga tgtttaaagc gaaaggacgt tatgtctgac aaacctggcg gcgagaaact
     4261 tctctactgt tctttctgcg gcaaaagcca gcacgaggtt cgcaagctga tcgccggccc
     4321 ctcggtgttc atttgcgacg agtgcatcga gctgtgcaac gacatcatcc gcgaggagag
     4381 ccacgccgag accaccggga aaggcgccaa gtccgatctt cccgttcccc acgaaattcg
     4441 cgagatcctc gaccagtacg tgatcggcca ggacaccgcc aagaagatcc tttcggtcgc
     4501 agtgtacaac cactacaagc ggctcaagac ggtgcagaag aaggacgacg tcgagcttgc
     4561 caagagcaat attcttttga tcggccctac cggctcgggc aagacgctgc tggcgcagac
     4621 gctggcgcgg ctgctgaacg tgcctttcgt gatggcggat gccaccacgc tgaccaaggc
     4681 cggctatgtc ggggaggacg tcgagaacat catccagaag ctgctgcaga agtgcgacta
     4741 cgacgtcgaa aaagcgcagc gcggcatcgt ttatatcgac gagatcgaca agatctcgcg
     4801 caagtccgac aacccctcga tcacccgcga cgtgtcgggg gaaggcgtgc aacaggcgct
     4861 gctgaagctc atcgaaggca ccatcgcctc ggtgccgccg cagggcgggc gcaagcatcc
     4921 gaaccaggaa ttcgtccagg tcgacaccac gaacatcctg ttcgtctgcg gcggcgcgtt
     4981 cgacggcctg gaccggatca tccgcgcccg ctccgacaag ggcggcatcg gttttggcgc
     5041 cgaagtgaag agcaaggaca accgcaagga tatcggagcg gtgctgcgcg gcgtcgagcc
     5101 cgaagacctg atcaagtacg gcctgatccc cgagttcgtc ggccgactgc cggtcgttgc
     5161 aacgctggat gaacttgatc aggccgcgct gatccaaatc cttaccgagc cgaaaaatgc
     5221 gctgctcaag cagtaccagc gcatgttcag catggaaggc gtcgagttgg aggtgcgttc
     5281 cgaggcgctg aaagcggtgg cgctgaaagc cctggcgcgg cgcaccggtg cgcgcgggct
     5341 gcgttcgatc ctggaacagg tgttgcttga catcatgtac gacctcccct ccctgaacaa
     5401 cgtggagaag gtggtcgtgg acgaaggcat gattaccggc gacgcgcagc cgatcctgct
     5461 ttactccgac cagcccaagg tggccggatc tgcctgagca gcggccaaaa tccggggccg
     5521 gcgcgcgtcg gagtccgcag cgccgccgaa ttgcccggct ttacaacgac ttggcgtccg
     5581 ggttgaaata cacgggcggc ccccaacctt ggaacgtatc cttgcccttt caggtgacca
     5641 atgacttatt ccgctgattc ttcccaaacg accgtcacct acccgctgtt gcctttgcgc
     5701 gacgtggtcg ttttccctca catggtcatt cccttgttcg tcgggcgccc gaaatcgatc
     5761 aaggcgctgg aaaccgcgat ggaagcgggc aagagcatcc tgctggtggc gcagaagtca
     5821 gccgcaaagg acgaaccgtc gcccgaggac ctgtacgaca tcggcagcat cgccaacatc
     5881 ctgcagatgc tcaagctgcc cgacggcacg gtgaaagtgc tggtcgaggg cgtacaacgc
     5941 gccaaggtcg tgtcggtcgc ggacgagaaa acgcattacg ccgccgaggc gctgccggtt
     6001 ccggccgatg cggtcgaaac caacgaggtg gaggcgatgc gccgcgccat gctcacccag
     6061 ttcgaccagt acgtgaaact caacaagaag attcctcccg agatcctcac gtcgctttcg
     6121 ggcatcgacg agcccggccg gcttgccgat accgtggccg cgcacctgcc gctgaaactc
     6181 gagcagaagc aggcggtgct ggaaatgttc gaggtcagga aacgcttgga gcatctgctg
     6241 tcgctcatgg aagccgagct cgacattctg caggtcgagc gccgcatccg tggacgcgtc
     6301 aagcgccaga tggagaagag ccagcgcgag tattacctca acgaacaggt caaggcgatc
     6361 cagaaggaac tcggcgacat ggaagagggc gccgacctgg acgaaatgga caagcgcatc
     6421 aaggcggcgc atatgccaaa ggaggcgcgc aacaaggctg aagccgagct caagaagctg
     6481 cggctgatgt cgcccatgtc agccgaggcc acggtggtgc gcaactacat cgaaacgctg
     6541 gtcgggctgc cctggaagaa gaagagcaag atcagcaacg atctggccaa ggcgcaggaa
     6601 atcctggacg ccgatcacta tgggctggac aaggtcaagg aacgcatcgt cgagtacctg
     6661 gcggtgcagc agcgcgtgga gaagctcaaa gcgccgatcc tgtgcctggt gggccccccg
     6721 ggcgtgggca agacctcgct tgggcagtcg atcgcgcacg cgaccaaccg caagttcgtg
     6781 cgcatgtcgc tgggcggcgt gcgcgacgag gccgagatcc gcggccaccg ccgtacttac
     6841 atcggctcga tgccaggcaa gctgctgcag aacatgtcga aaatcggcgt gaggaacccc
     6901 ctgttcctgt tcgacgaagt ggacaagatg gggcaggatt tccgcggcga tccggcttcc
     6961 gcgctgcttg aggtgctcga cccggagcag aacgacaagt tcgtcgatca ttacgtcgag
     7021 gtcgagtacg acctttcgga cgtgatgttc gtggctaccg cgaacaccat gaacatcccg
     7081 gcgcctctgc tggaccggat ggaagtgatc cgtctgtccg gctacaccga ggacgagaag
     7141 atcaacatcg cgatgcgtta cctgctgccg aagcagatga agaacaacgg cgtcaaggag
     7201 ggcgagctgt cggtctccga aagcgcc
//
LOCUS       DASQOP010000097         7891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1448771, whole genome shotgun sequence.
ACCESSION   DASQOP010000097 DASQOP010000000
VERSION     DASQOP010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7891
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1448771"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="VE030_03775"
     CDS             complement(<1..935)
                     /locus_tag="VE030_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZF22568.1"
                     /translation="MKNSMKHALVAAGLAAAATLAAPVAQAQVCSVWLVENSRFMGVP
                     FGAFMPDRAFVPGSTSPHNNINTVDLPVNVGVIKCGNELILYDSGWNQTDYHKMTGTG
                     HWSPLSKQIAQIGFDAKDVKKVIVGHGHWDHAGSLDEFPNAILYIQREELKGIEWALN
                     YPHPRISAVNDSPGGCARTPACGYPSKTMDSIYGKVLKGKAVIVDGEMDVLPGVKIHP
                     AFRAHTAGSQLLEVNTSIGKLVFGSDAYSSWEGIRDWEIANPQQTDTVQQFLAYEKCY
                     KITGGYQNCVCAHEPLSYTDKYPLTKNSWVGMNGGR"
     gene            complement(1027..1611)
                     /locus_tag="VE030_03780"
     CDS             complement(1027..1611)
                     /locus_tag="VE030_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22569.1"
                     /translation="MQTEVSGPQSTARTAALKGFGVGALVVGLIALAAVATFRETAKP
                     RQQEVATNSAFGQSERPAMTAAEEAYAHALWPVHAQVKQDAVKMTFAGLAYKMGDIKG
                     AVVKDRVAPLTAKFDSALAAMNKLQPPASMRDLHGEYVEAIKLYRDASVIMIKVAADG
                     RDEHLIEAQERSEKASGLTLKVGETLWPGEFKPN"
     gene            complement(1727..2527)
                     /locus_tag="VE030_03785"
     CDS             complement(1727..2527)
                     /locus_tag="VE030_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22570.1"
                     /translation="MITTLSLLAVGFFLGMRHATDADHVIAISTIVSRQRSLRGAALI
                     GALWGVGHTLTVAIVGGAIILFSIVIPPRTGLAMEFAVGVMLVVLGLLNLTGLNQWIR
                     DNVAPGSGPLHAHTHAHGDYAHTHAHGHGEANHGHPEDRTPQAWLDRKLGGIGLYQMA
                     RPLVVGIVHGLAGSAAVALLVLTAIKDPLWGMVYLFLFGAGTIAGMMLITVAIAAPFS
                     YSIERFPRFNAYLRVASGLLSLGFGLFLIYYIGYVDGLFTDHPTWSPK"
     gene            2517..2675
                     /locus_tag="VE030_03790"
     CDS             2517..2675
                     /locus_tag="VE030_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22571.1"
                     /translation="MVIKTGNSLMDLVLWGVKLLPARTGVKRRHENHEKLLRVLRRLR
                     IPVRVEHN"
     gene            2733..4535
                     /locus_tag="VE030_03795"
     CDS             2733..4535
                     /locus_tag="VE030_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HZF22572.1"
                     /translation="MTRCSPVRGFGLAALLLSGFAVNAAEWAGDLTPIGTQDWNAARA
                     AHLLERAGFGGTPEEIALYAAMTPQAAVRRLVYHKTIPNDLPAFDDSGAHDPGLEPFP
                     PSRPAATDLAKKTGESLGVKVKPAGNRRLQPVADRFFYWLRASRLETHRLAYWWANRM
                     LVTRRPLEEKMALFWHGHFATNEEKVRDYRKMLKQLELFQDKGTGSFRELLISVAQDP
                     AMLAFLDAAVNVKGAPNENFAREIMELFTMGVGNYTETDIREAARAFTGWNFVDLNFV
                     ISPDKHDSMVKTVLGRSGNFDGVAVIDVILAHPVTAEYIAGKIYRHFVREDISPELRK
                     ALGAKLRDANYQIAPLLETIFLSREFYSPASIGTRIKPPVELVVSTYRKMGLKEIPGV
                     PDFNEVTEMLGQILFYPPTVAGWAHGRSWITPGLLLARGNFVYDTVFPDINFLPPDRY
                     PGNDYQIAAVSEKLAMGLDVTTATKPDAKEMTSISMQMADRDEDFNTRLASYRGWQMA
                     IQKVKPIPRATAQLDLAGMVRAAGCRNAQQAVDVLLARFVSVPVEAGTRARIGKFLES
                     ELGTGDLSAAGTFLEEPLRSTLHLILSLPEYQLG"
     gene            4615..5850
                     /locus_tag="VE030_03800"
     CDS             4615..5850
                     /locus_tag="VE030_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZF22573.1"
                     /translation="MKPTIHLSTKSWTRRDVLRACLCGAGAGALNGMPLVFSRALAAD
                     ATASNRILVVLELSGGNDGLNTLVPHGDDAYYRHRPRIGIRPDKLRRIDERFGFNPGM
                     AGFEGLYKDGRMAVVHGCGYAQPSFSHFTSMAYWHTGAPNSGEETGWVGRLADAIDTQ
                     ATPNFIVNIDEAQSLAVSARNHVPVVFDDPERFGRKGLYQTRPLLDQVSDATHDANPS
                     RRYLLEVAKSARAASQLVRDAWAKYKTPVDYGIVAVDLPKIASLIAAGMPTRLYYTSY
                     RHNAFDTHVQQPDLHQRLLTYVSDAVAGFMKDLQRIGRADDVVMLVFSEFGRRVPENT
                     SLGTDHGTANHMYLIGKPVKGGHYGTPPKLDALDEGDNLVFTTDFRRVYASVIDGWLG
                     YGDTRKLLSGNFQTFPIFG"
     gene            6038..6421
                     /locus_tag="VE030_03805"
     CDS             6038..6421
                     /locus_tag="VE030_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZF22574.1"
                     /translation="MKNMDIRSKTYGDVLVLSPTGRIDHANSEVFRRALNPLIERCSA
                     NGQRLVLDLAGVDYVSSAGLRCFMLAEKQAKSQGGAIVVAAMQPVVNEIFEISRFTLV
                     FETFATVRDALAKVSPAALKAFDAA"
     gene            6427..7386
                     /locus_tag="VE030_03810"
     CDS             6427..7386
                     /locus_tag="VE030_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZF22575.1"
                     /translation="MRVRFWGTRGSIPVALTSADIRHKIEQALALAIASGVDSPEKLQ
                     NFIEHDLGFSISHTYGGHSSCVELETGRAEYLVCDMGSGARAFGNRIMSRRDGGPPPV
                     INVIMSHVHWDHIMGFPFFAPAYVPGARIRIHGCHDVLEQAFRLQQSAPCFPVDFSRL
                     AADIEFTQLEAGASYDVAGFRVTPKLQRHTGGSYGYRFERDGKTVVYSTDSEHKLEDP
                     AETRGFVEFFRNADLVIFDAMYSLLDAVSMKEDWGHSSNIVGVEMAQMANVKHLCLFH
                     HEPAFDDDRIESVLNETIRLEKLTRTEQPLKITAAYDGMEIEL"
     gene            7383..>7891
                     /locus_tag="VE030_03815"
     CDS             7383..>7891
                     /locus_tag="VE030_03815"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HZF22576.1"
                     /translation="MTQDPQASAGGGDTPPGGQSRSWSERALTRALVGGQGRPLGAAV
                     LVFLLLLSFLPDLRVLTQLRLALFDSYQTIAPRVRVSAPAVIVAIDEKSLNAIGQWPW
                     PRTVMAELIEAIGETGPAAIGVDVLMPEPDRMSPVNLAGLIERLDAGLARRLAQLPDN
                     DAVLAESLRR"
BASE COUNT         1480 a         2543 c         2410 g         1458 t
ORIGIN      
        1 cggccgccgt tcatccccac ccaggaattc ttggtgagcg ggtatttgtc ggtgtaggag
       61 agcggctcgt gggcgcacac gcagttctga tagccgccgg tgatcttgta gcacttctcg
      121 taggccagga actgctgcac cgtgtcggtc tgctgcgggt tggcaatttc ccagtcgcgg
      181 atgccttccc aggacgagta cgcgtcgctg ccgaacacca gcttgccgat cgaggtattg
      241 acttccagca actgcgagcc ggccgtgtgc gcacggaagg ccggatggat cttcacgccg
      301 ggcagaacgt ccatttcgcc gtcgacgatc accgccttgc ccttcaacac cttgccgtag
      361 atcgagtcca tggtcttgga cgggtaaccg caggccggag tgcgggcaca gccgccgggt
      421 gaatcattta ctgcggaaat gcgcgggtgc ggatagttca acgcccactc gatgcccttc
      481 agttcttccc tctggatata cagaattgca ttcgggaact catccagcga accggcatga
      541 tcccagtggc cgtggccgac gatgactttt ttcacgtcct tcgcatcgaa gccgatctgc
      601 gcaatctgct tggacagcgg cgaccagtgc ccggtgccgg tcatcttgtg gtagtcggtc
      661 tggttccagc cggagtcgta caggatcagt tcgttgccgc acttgatcac gccgacgttg
      721 accggcaggt cgacggtatt gatgttgttg tgcgggctgg tgcttccggg cacgaaggcg
      781 cggtcaggca tgaaggcccc aaacggcact cccatgaagc ggctattctc gaccaaccac
      841 accgagcaga cttgcgcctg cgccaccggt gccgccagcg ttgccgcagc agcaaggccc
      901 gcggcaacca acgcgtgttt catactgttc ttcatttttt actcctccta gacaattgga
      961 ctgctcgatg cgacttcttc ttgttcccgc acgcgtgcct ggagacgcgc gtccggaaat
     1021 ccttgctcaa ttcggcttga actcacccgg ccacaatgtc tcgcccacct tgagagtcag
     1081 gcccgatgct ttttccgatc tttcctgcgc ttcaatcagg tgctcgtcgc gcccatccgc
     1141 cgccaccttg atcatgatta cggacgcatc ccgatacagc ttgatggcct cgacgtattc
     1201 gccgtgcaag tcccgcatcg acgccggcgg ttgcaacttg ttcatcgctg ccagcgccga
     1261 gtcgaacttc gccgtaagcg gcgcgacccg atctttcact acggcaccct tgatgtctcc
     1321 catcttgtat gcgagacccg caaaggtcat tttcaccgcg tcctgcttca cctgcgcgtg
     1381 aaccggccac aacgcgtgcg cgtacgcttc ttccgcagcc gtcatcgcgg gacgctccga
     1441 ctgaccgaac gccgaattcg tcgcgacctc ttgctgcctg ggtttggcgg tttcgcggaa
     1501 tgttgcgaca gccgctagcg caatcaatcc gactaccagt gcgccgacac caaaaccttt
     1561 caatgccgcc gtgcgggccg tactttgcgg cccgcttact tctgtttgca ttgtgaaaat
     1621 ttagtctccg aacgacttag cgtcaatgct gcgccgcagt aactacttct taatttacaa
     1681 gcactaattc ttaagtttca gtttgaaata ttcgccggga gatttcttac ttcggcgacc
     1741 acgtcgggtg gtcggtaaac aggccatcga cgtagccgat gtagtagatc aggaacagtc
     1801 cgaatcccag cgacagcagc ccggaagcca cgcgcagata ggcgttgaag cgcggaaagc
     1861 gctcgatcga ataggaaaac ggcgcggcga tcgccacggt gatcagcatc atgccggcga
     1921 tggtgccggc gccgaacagg aacaggtaca ccatgcccca caacggatcc ttgatcgcag
     1981 tgagtaccag cagcgccact gccgccgatc cggccagtcc gtgcacgatg cccaccacaa
     2041 gcgggcgcgc catctgatag agcccgattc cgccgagttt tcggtccagc caggcctgcg
     2101 gtgtccggtc ctcggggtgt ccgtggttcg cctcgccatg gccgtgcgca tgggtatgcg
     2161 cataatcacc atgggcatgg gtatgggcgt gaagcgggcc ggaacccggc gccacattgt
     2221 cgcggatcca ttggttgagg ccggtcagat tgagcaagcc gagcacgacc agcatgaccc
     2281 cgacggcgaa ctccatcgcc agcccggtgc gcggcggaat gacgatggag aaaagaatga
     2341 tcgccccgcc gacgatcgcc accgtcaacg tgtggcccac tccccacagc gcgccgatca
     2401 gcgccgcgcc gcgcaacgaa cgctggcggc tgacgatcgt cgagatcgcg atgacatggt
     2461 cggcatcggt ggcgtgacgc atgccgagaa agaagccgac tgcaagcagg gacagcgtgg
     2521 tgatcaagac cggaaacagc ctgatggatt tggttttgtg gggcgtgaag ctactcccgg
     2581 cgcgcacggg cgtcaagcga cgtcatgaaa atcatgaaaa gctcctgcgg gttttgcgtc
     2641 ggctccgtat tccggtccgg gtagagcata attgaaggcc ttacggtcgc cggccgatac
     2701 cggaccgcat gaccatgtgc cagggagacg ccatgacgag atgttcaccc gtgcgcggat
     2761 tcggccttgc cgcgctactg ctgtcgggct tcgctgtgaa cgccgcggag tgggcgggcg
     2821 acctcactcc gatcggaacg caggactgga atgccgcacg agctgcgcat ctgctggagc
     2881 gtgccggctt cggcggcaca ccggaagaga tcgcgctgta cgccgcaatg actccacagg
     2941 ccgcggtccg gcggctggtc taccacaaga ccatcccgaa cgatctgccg gccttcgacg
     3001 actccggtgc gcacgatccc gggcttgaac ctttcccacc ttcccggccg gccgcaaccg
     3061 atctcgccaa gaagaccggc gaatccctcg gcgtcaaagt caagcccgcg ggcaatcggc
     3121 gcctgcagcc ggttgccgac cggtttttct actggctgcg cgccagccgg ctggagacgc
     3181 atcgcctcgc ctactggtgg gcgaaccgca tgcttgtcac ccgacgtccc ctggaagaaa
     3241 agatggcgtt gttctggcac ggacacttcg ccaccaacga agagaaggtg cgcgattacc
     3301 gcaagatgct caagcagctc gagttgttcc aggacaaggg caccggcagc tttcgcgagc
     3361 tgctgatcag cgtggcgcag gacccggcga tgctcgcctt cctcgacgcc gcagtgaacg
     3421 tgaagggcgc gccgaacgag aacttcgcac gcgagatcat ggagctgttc accatggggg
     3481 tcggcaacta caccgaaacc gacatccgcg aagccgcgcg cgcctttacc ggttggaatt
     3541 tcgtcgacct gaacttcgtg atctcgcccg acaagcacga cagcatggtg aaaacggtgc
     3601 ttggtcgcag cggcaacttc gacggcgtgg cggtgatcga cgtcatcctc gcccatccgg
     3661 tgaccgccga gtacatcgcc ggcaagatct accgccattt cgtgcgcgag gacatttctc
     3721 cggagctgcg caaggctctg ggggccaagc tgcgcgatgc gaactatcag atcgcgccgc
     3781 tgctggagac gatatttctt tcccgcgagt tctacagtcc cgcgtcgatc ggcacccgca
     3841 tcaagccgcc ggtcgagctg gtggtgtcga cttacaggaa aatggggctg aaagaaatcc
     3901 ccggagtgcc ggacttcaac gaagtgaccg aaatgctcgg ccagatactg ttctacccgc
     3961 ccacggtggc cggctgggca cacggcagaa gctggatcac gccgggcctg ctgctggcac
     4021 ggggcaactt cgtgtacgac acggtgtttc ccgatatcaa cttcctcccg cccgatcgct
     4081 acccgggcaa cgactaccag atcgccgccg tgagcgagaa gctggcgatg ggtttggacg
     4141 tgacgaccgc gaccaagcct gacgccaagg agatgacttc gatatcgatg cagatggccg
     4201 accgcgacga ggatttcaac acccggctgg cgagctaccg tggctggcag atggcgatcc
     4261 agaaagtgaa gccgatcccg agggcaactg cgcaactgga tctcgccggc atggtgcgcg
     4321 cggccggctg ccgcaacgcg cagcaagcag tggacgtgct gctggctcgc ttcgtttcgg
     4381 taccggtgga agccggcacc cgcgcacgga tcgggaaatt tctcgaaagc gaactcggta
     4441 ccggcgatct gtccgcggcc ggcactttcc tggaagagcc gttgcgatcg acgctgcacc
     4501 tgatcctgtc gttgccggag taccaactgg gctagaccac cacctctccc tgaccctcca
     4561 cccacctcac ccccgccctc tccccccaga gggcggagag ggagcgagaa aaccatgaaa
     4621 ccgacaattc atctctcgac aaaatcgtgg acacgacgcg atgtgctgcg cgcatgcttg
     4681 tgcggcgcag gggccggtgc actcaatggc atgccgctgg tgttctcgcg cgcgctcgcc
     4741 gcggatgcca cggcctcgaa tcggatcctc gtggtcctcg agctatccgg aggcaacgac
     4801 ggcctcaata cgctggtgcc ccatggcgac gacgcctact accgccatcg tccccgaatc
     4861 ggcatccgcc cggacaagct gcgcaggatc gacgagcgct tcggtttcaa tccgggaatg
     4921 gccggcttcg aaggcctcta caaggacggc cggatggcgg tggtgcacgg ttgcggctac
     4981 gcgcagccgt cgttctcgca tttcacctcg atggcttatt ggcacacggg cgcgcccaat
     5041 agcggagagg aaaccggctg ggtcggccgg ctcgccgatg ccatcgacac acaggccacg
     5101 ccgaatttca tcgtcaacat cgacgaggcg cagtcgctgg ctgtgagcgc acgcaatcac
     5161 gtcccggttg tgttcgacga tccggaacgc ttcggccgca agggcctgta ccagaccagg
     5221 ccgctgctgg accaggtttc cgacgcgacg catgacgcga acccctcgcg gcgttacctg
     5281 ctcgaagtgg cgaaaagcgc gcgcgcggcc tcgcagctgg tgcgcgatgc atgggcgaag
     5341 tacaagacgc cggtggatta cggcatcgtc gcggtcgatc tgccgaaaat cgcgtcgctg
     5401 atcgccgcgg gcatgccgac acggttgtac tacacgtcgt atcggcacaa cgctttcgac
     5461 acccacgtgc agcaaccgga cttgcatcag cggttgttga cctacgtctc cgacgcggtg
     5521 gccggattca tgaaagacct gcagcgcatc ggccgcgccg acgacgtggt gatgctggta
     5581 ttttccgagt tcggacggcg ggttcccgag aacaccagcc tcggcaccga tcatggcacc
     5641 gccaaccaca tgtacctgat cggcaagccg gtgaagggcg gccactacgg cacgccaccg
     5701 aagctcgacg cgctcgacga aggcgacaac ctggtgttca ccaccgattt ccgccgcgtc
     5761 tacgccagcg tcatcgatgg ctggctgggc tacggcgaca cgcgcaagct gctctccggc
     5821 aatttccaaa ccttccccat cttcggctga gccggcggcg cgggcagcct gttcgcgccg
     5881 ttttcgggtt taatatggtg ttggcaggtt ctaatccgtt cgcgtgcgcg ttgcgcctgg
     5941 cagaactcct cagagcaagc cagcccaagc gcggccgggc cgggccggca tgcgccgtgc
     6001 ataacctccg acatccagcg gccactgcag cgcgcgaatg aaaaatatgg atatccgcag
     6061 caagacttac ggcgacgtgc tggtgctctc gcccaccggc aggatcgacc acgccaacag
     6121 cgaagtattc cgccgggctc tgaatccgct gatcgaacgc tgcagcgcca atggccagcg
     6181 cctggtgctg gatctcgccg gcgtggacta cgtcagcagc gccgggctgc gttgtttcat
     6241 gctcgcggaa aagcaggcca agtcgcaggg tggtgccatc gtggttgcgg cgatgcagcc
     6301 cgtggtgaac gaaatcttcg agatcagccg gtttaccctg gtattcgaga ctttcgcaac
     6361 ggtgcgcgat gcgctggcga aggtttcgcc cgcagcgcta aaggccttcg acgccgccta
     6421 gtgaccatgc gggtacgctt ctgggggacg cgcggctcga tcccggtcgc gctgacttcc
     6481 gccgacatcc ggcacaagat cgagcaggcg ctcgccctgg ccatcgccag cggcgtggac
     6541 tcccccgaaa agctgcagaa cttcatcgag cacgacctgg gtttcagcat ttcccacacc
     6601 tacggcgggc attcgtcgtg cgtggaactc gaaaccgggc gcgccgaata cctggtctgc
     6661 gacatgggca gcggcgcacg tgcgttcggc aaccgcatca tgtcgcgacg cgacggcggg
     6721 ccgcctccgg tcatcaacgt gatcatgtcc cacgtgcatt gggaccacat catgggcttt
     6781 cccttcttcg cccccgccta tgttccgggg gcgcgcatcc gcatccatgg ctgtcacgac
     6841 gttctcgagc aggcgttccg gctgcagcag tctgcgcctt gcttcccggt ggatttttcg
     6901 cggctcgccg ccgatatcga attcacgcaa ctcgaggccg gcgccagcta cgacgtcgcc
     6961 ggcttcaggg tgacaccgaa gttgcagcgg cacaccggcg gctcctacgg ttaccgcttc
     7021 gagcgcgacg gaaaaaccgt ggtgtactcg accgattccg agcacaagct cgaagacccg
     7081 gccgaaaccc gcggcttcgt cgagttcttc cgcaatgcgg acctggtgat cttcgacgcc
     7141 atgtactctc tgctggacgc agtgtcgatg aaggaggact ggggacactc gagcaacatc
     7201 gtcggcgtgg agatggcaca gatggcgaac gtgaagcatc tgtgcctgtt ccatcacgag
     7261 cctgcgttcg acgacgatcg cattgaatcg gtgctcaacg aaaccatccg cctcgagaag
     7321 ctgacccgca cggagcagcc gctgaagatt acggctgcgt acgacggcat ggaaatcgag
     7381 ttgtgacgca agatccacag gccagtgcgg gcggcgggga cacgccgccg ggcggacaaa
     7441 gtcgctcctg gagcgaacgc gcgctcacgc gcgcgctggt cggcggccag gggcggccgc
     7501 tgggcgccgc agttctggtg ttcctgctgc tgctctcgtt cttgcccgat ttgcgcgtgt
     7561 tgacccagct tcggctggcg ctgttcgaca gttaccagac catcgcgccg cgcgtacgtg
     7621 tttccgcccc ggcggtgatc gttgccatcg acgagaaaag tctcaacgca atcggccagt
     7681 ggccgtggcc gcgcaccgtg atggcggagc tcatcgaagc aatcggagaa accggtccgg
     7741 ctgcgatcgg cgtggacgtg ctgatgccgg aacccgaccg catgtcgccc gtcaacctag
     7801 cggggctgat cgagcgcctg gacgccggac tcgcccgccg cctggcacag ttgcccgaca
     7861 atgacgccgt ccttgcggaa tcgctgcgcc g
//
LOCUS       DASQOP010000098         5900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1248709, whole genome shotgun sequence.
ACCESSION   DASQOP010000098 DASQOP010000000
VERSION     DASQOP010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5900
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248709"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VE030_03820"
     CDS             <1..545
                     /locus_tag="VE030_03820"
                     /inference="COORDINATES: protein motif:HMM:NF018928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HZF22577.1"
                     /translation="QSMQNLFILTDGTWDRRVYLPAYVRRYPFCMAKVTLDGETQKER
                     IVCVEASAVQDGGEPLYDEAGNTLPQWSELEKLIFDYESDLLRAEELCKLLAGLELLE
                     PFAMKAEVEGFTMQLEGMHRVARNALESLADDKLRQLLVAGVLEKIYSHLLSLDNFRR
                     LLNRRSFFAVSPPTKPRDLN"
     gene            complement(538..1332)
                     /gene="lgt"
                     /locus_tag="VE030_03825"
     CDS             complement(538..1332)
                     /gene="lgt"
                     /locus_tag="VE030_03825"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HZF22578.1"
                     /translation="MLIHPQFDPVALHLGPLSVRWYGLMYLLAFVIVLMLGRYRIRTQ
                     PWLSWANRDLDDILFLGVLGAVLGGRLGYVLFYKFSEYLYDPVRILFLWEGGMSFHGG
                     FLGVLVAMLWFARSRHKSWLAVTDFIAPLIPLGLGAGRIGNFINAELWGRPANVPWAM
                     VFPHVDNLPRHPSQLYEFILEGLVLFALLWWFSRRWRPVGAVSGVFLIGYGTLRFLVE
                     FTREPDGFLGLLALGLSMGQWLSLPMVIGGIALLAWSNRRRTNRVS"
     gene            complement(1542..2237)
                     /gene="mtgA"
                     /locus_tag="VE030_03830"
     CDS             complement(1542..2237)
                     /gene="mtgA"
                     /locus_tag="VE030_03830"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HZF22579.1"
                     /translation="MKRLLRWTWRSIALVLAAIVVYQFWIFGNVVWWIWFNPSNSAFM
                     ERRLEVLQEDNPGAALRHQWVPYERISINLKRALIAAEDAKFLDHDGFDWEAIQKAYE
                     KNIIKGKIVAGGSTISQQLAKNLFLSGKRTPWRKAQEALITVMIEKVMTKRRIFEIYL
                     NIIEWGNGVFGAEAAARHYYDTSAAYLDVEQAARLAAMVPKPRYYDRVRYTPLLEQRS
                     GIILERMYYVEVP"
     gene            complement(2234..3064)
                     /gene="aroE"
                     /locus_tag="VE030_03835"
     CDS             complement(2234..3064)
                     /gene="aroE"
                     /locus_tag="VE030_03835"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HZF22580.1"
                     /translation="MTDRYAVIGSPIAHSKSPQIHAAFARQTGQDIEYTCLLAPPDGF
                     RRTVENFRAAGGRGANVTVPFKLEAFDLADEVSQRAKEAQAANFLNFAGGRIRADNTD
                     GAGLIRDITGNLSFSIAGQRVLLMGAGGAARGALQPLLAQQPAILTIANRTVEKALQL
                     AEAFRYHPSAASSVLSGLRYDELAGHHFDLLINATSSSLQDELPPLPAGVFADASLAY
                     DMMYGKGLTPFLAFAQFQGAARLADGLGMLVEQAAESFFLWRGVRPETRPVIELLRKQ
                     "
     gene            complement(3061..3987)
                     /locus_tag="VE030_03840"
     CDS             complement(3061..3987)
                     /locus_tag="VE030_03840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HZF22581.1"
                     /translation="MPDISIRTLDTRGSLAAGLSLLPPPSTGDILPVTLAFSIVFHAV
                     LLLITFASPNFAGRNFVPHLEVVLVNAKSSSKPLKADALAQASLDGGGNTEAKTRAST
                     NLPKVNNLEPSQEVQLAANRVQRLEEEAQRLLRLAQGSHPSATDTRPAPTAEQQERLE
                     DPALQQQRLLIVQLEARIEKEMKEYQELPRRKAIGSRTEGVVYAEYVDKWRRRIELVG
                     TSNYPEEAKRRKIYGSLLLTVHIKADGSVEKVEIDRSSGHRLLDAAARRVVELAGPFP
                     PFPAVVRKDWDILSISRTWMFTRSDLELVASP"
     gene            complement(4043..>5900)
                     /locus_tag="VE030_03845"
     CDS             complement(4043..>5900)
                     /locus_tag="VE030_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HZF22582.1"
                     /translation="QRMNVFFEEDGGFKAGTVLADNNSSLQVETLHGKRAKVKAAAVL
                     IRFEHLTLSQFMAEAHKVSEEIDPAFLWECCGEAEFSFEQLGREYFGREPKAQEGAGL
                     LMRLHATPTHFYERGKGRYKAAPPDALKSALASIERKQKQAAQQARYLEQLGRFELPE
                     EFRPMLKELLYRPDRSTIEFKALDQAAVRERLAIPKVLEKCGALPSSHDYHCGRFLFE
                     YFPRGSGFDTVLTAADPGVLETAEVTAFSIDDATTTEIDDAFSVTRLANGNWQVGVHI
                     AAPALGFTVGSPLDVEAAKRLSTVYFPGNKITMLPDSVIDQYTLSEGRDRPALSMYVE
                     VTPELEIVGNRSAVERVGIAANLRSDTLELAFNQEQVTAGQVDCPFGAELLLLWRIAT
                     RLEQRRGKEEPGLTRSDYNFYVDDDRVRIVERKRGSPTDRVVSELMILVNTEWGRQLA
                     VNEIPALYRAQGNGKVKMSTLPAPHQGLGVSQYIWASSPLRRYVDLVNQRQLLALYRH
                     ETPPYPPKDERLLVILRDFELAYESYAEFQRNMERYWCLRWLIQEGAATAEAEVLREE
                     LVRIDRIPLVVRVPSLPPLAPGKRVELAISDIDLIELTLHCEFKRQTDEIAR"
BASE COUNT         1108 a         1862 c         1820 g         1110 t
ORIGIN      
        1 tgcagtccat gcagaatctt ttcatcctca ccgacggcac ctgggaccgg cgggtttatc
       61 tgcccgccta tgtgcgacgc tacccttttt gcatggccaa agtgacgctg gacggcgaaa
      121 cgcagaagga gcgcatcgtg tgcgtggagg cgagcgccgt tcaggacggc ggcgagccgc
      181 tgtacgacga agccggcaat acgcttccgc aatggagcga actggaaaag ctgattttcg
      241 actatgagag cgacctgttg cgcgcagagg agttatgcaa gctgctggcc gggcttgaat
      301 tgctggagcc gttcgcgatg aaagcggagg tagaaggttt caccatgcaa ctggaaggca
      361 tgcatcgcgt tgcgagaaat gcgctggaat cgctggcgga cgacaagctg cgccagctgc
      421 tcgtcgcagg cgtgctcgaa aagatttatt cacacctgct gtcgctggac aacttccggc
      481 gcctgctcaa ccgccgcagc ttctttgcgg tcagcccgcc gaccaaaccg cgcgacctca
      541 actaaccctg tttgtcctgc gccggttgga ccacgcaagc agcgcgatac cgccgataac
      601 catcggcagg ctcagccact gacccatcga caatcccagc gcaagcagtc ccaggaaccc
      661 gtccggctcg cgggtgaact cgaccaggaa tcgcagggtc ccgtaaccga tcaggaacac
      721 cccggagacc gcgccgaccg gacgccagcg cctggagaac caccacagca acgcgaacag
      781 caccaggccc tcgaggatga attcgtagag ctgcgacgga tggcgcggca ggttgtccac
      841 gtgcgggaaa accatcgccc agggaacgtt ggcgggccgt ccccatagtt ccgcattgat
      901 gaagttgccg atccttcctg cgcccagccc gagcggaata agcggcgcga tgaagtcggt
      961 cacggccaac cagcttttgt gccgggagcg cgcaaaccac agcatcgcga ccagcacgcc
     1021 caggaagccg ccgtggaagg acatgccgcc ttcccacagg aagaggatgc gcaccggatc
     1081 gtacagatac tcggagaact tgtagaacag cacgtagccg agccgcccgc ccagcaccgc
     1141 gccgagcacg ccgagaaaca ggatgtcgtc gagatcccgg ttggcccagc tcagccatgg
     1201 ctgggtcctg atgcgataac gcccgagcat cagcacgatg acgaaggcca gcaggtacat
     1261 caacccgtac cagcgcaccg acagcgggcc gagatgcagg gctacgggat cgaattgcgg
     1321 atggatgagc atgacggcat gggagaagtt ggcgaattat acgtgagcga tcttcaaccc
     1381 cggcagcttc gcgacggaaa aacccaaccc aagaatctcg cgaccaaaat caaaatctca
     1441 ccacgaaggc acgaagacac gaagagaatc gtcaacatca tttcttcttc gtgtcttcgt
     1501 gccttcgtgg tgagaaatcg ggtttcgcct gcggccggat ttcagggaac ctccacgtag
     1561 tacatgcgct ccaggatgat gccgctgcgc tgctccagca gcggggtgta gcgtacccgg
     1621 tcgtagtagc gcggtttcgg caccattgca gcgagccgcg ccgcctgctc gacgtcgaga
     1681 taggccgcgc tggtgtcgta gtagtgacgt gcagcggctt cggcgccgaa cacgccgttt
     1741 ccccattcga tgatgttgag ataaatctcg aaaatgcggc gcttcgtcat taccttctcg
     1801 atcatgacgg tgatcagcgc ttcctgtgcc ttgcgccacg gcgtgcgctt gccggaaagg
     1861 aacaggttct tggccagttg ctggctgatg gtcgaacccc cggcaacgat ctttcccttg
     1921 atgatgttct tttcgtaggc cttctggatc gcctcccagt caaagccgtc gtggtcgaga
     1981 aacttggcat cttcggccgc gatcagtgcg cgcttgagat tgatcgagat gcgctcgtag
     2041 ggaacccatt ggtggcgcag cgccgccccg gggttgtcct cctgcaggac ttccagccgt
     2101 cgttccatga acgcgctgtt ggaaggattg aaccatatcc accagaccac gttgccgaag
     2161 atccagaact ggtagacgac gatcgcggcg agcaccagag cgatgctccg ccaggtccag
     2221 cgcagcaggc gcttcattgc tttcgcagca gttcgatgac cggacgggtt tcggggcgca
     2281 cgccgcgcca caggaagaat gactccgcgg cctgctcgac cagcatgccg agaccgtcgg
     2341 caaggcgcgc agcgccctga aactgcgcaa acgcgagaaa aggcgtaagt cccttgccgt
     2401 acatcatgtc gtaggcaagc gaggcgtcgg cgaacactcc cgccggcaac ggcggcagtt
     2461 cgtcctgcag gctgctggaa gtggcgttga tcagcagatc gaagtgatgt cccgccagtt
     2521 cgtcgtagcg caatcccgac aacaccgagg acgccgccga tggatggtag cgaaatgcct
     2581 cggcgagctg cagcgccttt tccaccgtgc ggttggcgat tgtcaggatt gccggctgct
     2641 gcgccagcag tggctgcagc gcgccccgcg ccgcgcctcc cgcacccatc agcagcaccc
     2701 gctggccggc gatgctgaaa ctgagattgc cggtgatatc gcggatgagg ccggcgccgt
     2761 cggtattatc ggcgcggatg cgcccgccgg cgaagttgag gaaattcgcc gcctgcgctt
     2821 ccttggcgcg ctgcgatact tcgtcggcca ggtcgaaggc ttcaagtttg aaaggcacag
     2881 tcacgtttgc gcccctgccg cccgccgcac gaaagttttc cacggtgcgg cgaaagccgt
     2941 cgggtggcgc cagcaggcac gtgtattcga tatcctgtcc ggtctggcgg gcaaacgccg
     3001 cgtgaatctg cggtgacttg ctgtgggcaa tcgggctgcc gattaccgcg tagcggtcgg
     3061 tcatggagaa gcgacgagtt cgaggtcgct gcgggtaaac atccaggtgc gcgatatgct
     3121 cagtatgtcc cagtccttgc gcaccacggc cgggaagggc gggaacggtc cggcaagttc
     3181 gaccacgcgc ctggccgccg cgtcgagcaa gcgatggccg gatgagcggt cgatctcgac
     3241 tttctccacg ctgccgtccg ccttgatgtg cacggtcagc aacaacgagc cgtagatctt
     3301 gcgccgcttc gcttcctccg ggtagttgct ggtgccgacc agttcgatgc gccggcgcca
     3361 cttgtccacg tattcggcgt aaacgacacc ctcggtgcgc gacccgatgg ctttgcggcg
     3421 cggcagctcc tggtattcct tcatttcctt ttcgatcctc gcctcgagtt gcacaatcag
     3481 caggcgctgc tgctgcagtg ccgggtcttc gaggcgctcc tgctgttcgg ccgtgggtgc
     3541 aggccgggtg tctgtcgcgg atgggtggct gccttgagcc aggcgtagca gccgctgcgc
     3601 ttcctcctcg agcctctgca ccctattcgc tgccaactgc acttcctggg acggttcaag
     3661 gttgttcacc ttcggcagat tggtgctcgc cctggttttg gcctcggtat tgccgccgcc
     3721 gtcgaggctg gcctgggcca gcgcatcggc cttgagcggc ttcgaggatg atttcgcgtt
     3781 gaccagcacc acctccaggt ggggaacaaa attcctcccg gcgaaattcg gcgacgcgaa
     3841 cgtgatcagc agtaacacgg cgtggaagac gatggagaac gccagcgtaa ccggcaggat
     3901 gtcgccggtg gagggcggtg gaaggagcga caggccggcg gcgaggctgc ctcgggtgtc
     3961 gagcgttctg atcgagatat ccggcaaatc gctaggcgaa gtagggacgc tgcgccaatt
     4021 ctaaataact gcggggcaac tgtcagcgcg caatttcatc cgtctgccgt ttgaactcgc
     4081 aatgcagcgt caattcaatc aggtcgatgt cggaaatcgc cagttccacc cgctttccgg
     4141 gtgcaagcgg cggtaacgac ggcacccgga ctaccagcgg gatgcgatcg attcttacca
     4201 gttcctccct gagcacttcc gcctccgccg ttgcggcgcc ttcctggatc agccagcgca
     4261 agcaccagta gcgctccatg ttgcgctgaa actccgcata ggattcgtaa gccagctcga
     4321 agtcccggag gatcaccagc agccgctcgt ccttcggcgg gtaaggcggc gtctcgtggc
     4381 gatatagcgc cagtaactgc cgctggttca ccaggtccac gtaacggcgc agcggcgaac
     4441 tcgcccagat gtactgggaa acgcccagcc cttgatgtgg ggccggcagc gtcgacatct
     4501 tcactttgcc gtttccctgc gcccggtaca gcgccggaat ctcgttgacc gcgagctgcc
     4561 ggccccattc ggtgttcacc agaatcatca gttcggaaac gacccggtcg gtgggcgatc
     4621 cgcgcttacg ctcgacgatg cggacccggt cgtcgtcgac gtagaagttg tagtccgagc
     4681 gcgtgagccc cggttcctct tttccgcggc gttgttccag ccgggtcgca atccgccaca
     4741 acagcagcag ttccgcgccg aacgggcagt ccacttgccc cgccgtcacc tgctcctggt
     4801 tgaacgccag ctcaagggtg tcgctgcgca ggtttgcggc gatcccgacc cgttcgaccg
     4861 cgctgcggtt gccgacgatt tccagttcgg gcgtcacttc cacgtacatc gacagcgccg
     4921 gacgatcacg gccctccgaa agagtgtact ggtcgatcac ggaatccggc agcatggtga
     4981 tcttgtttcc cgggaagtag acggtggaaa gccgctttgc cgcttcgaca tccagcggcg
     5041 atccgaccgt aaacccgagc gcgggcgcgg cgatgtgcac gcccacctgc cagttgccgt
     5101 tcgcgagcct ggtgaccgag aacgcatcgt cgatctccgt ggtggtcgca tcgtcgatgc
     5161 tgaacgccgt tacttccgcg gtttccagta cgccgggatc ggcggccgtg agcaccgtat
     5221 cgaagccgga acccctggga aaatattcga acaggaaacg gccgcagtga tagtcgtggc
     5281 tggacggcag cgcgccgcat ttttccagca ccttcggaat cgccagccgc tcccgcaccg
     5341 cggcctggtc cagcgccttg aattcgatgg tgctcctgtc gggccggtaa agcagttcct
     5401 tcagcatcgg ccggaactcc tccggcaatt cgaaccggcc aagttgctca aggtagcggg
     5461 cctgttgcgc cgcctgtttt tgcttacgct cgatgctggc cagcgccgac ttcaacgcgt
     5521 ccggcggtgc ggccttgtaa cggcccttgc ccctctcgta gaaatgcgtc ggggtagcgt
     5581 gcagccgcat cagcaacccg gcgccttcct gcgccttggg ctcgcgcccg aagtactcgc
     5641 gccccagctg ctcaaacgag aactcggcct cgccgcagca ttcccagaga aacgccggat
     5701 cgatttcctc ggagaccttg tgcgcctccg ccatgaactg cgacagcgtc aagtgctcga
     5761 agcggatcag caccgccgcg gcctttacct tggcgcgctt gccgtgcagg gtttcgacct
     5821 gcagcgagct gttgttgtcg gcgagtacgg tgccggcctt gaagccgccg tcctcttcga
     5881 agaaaacgtt catgcgctgc
//
LOCUS       DASQOP010000099        25047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2655586, whole genome shotgun sequence.
ACCESSION   DASQOP010000099 DASQOP010000000
VERSION     DASQOP010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 25047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25047
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2655586"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..132
                     /locus_tag="VE030_03850"
     CDS             <1..132
                     /locus_tag="VE030_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22583.1"
                     /translation="LQIKTGSLSRSDRLAKYNQLLRIEEDLGDSASYAGRDAFYQIN"
     gene            136..444
                     /gene="ftsB"
                     /locus_tag="VE030_03855"
     CDS             136..444
                     /gene="ftsB"
                     /locus_tag="VE030_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HZF22584.1"
                     /translation="MRFLAFALVVLIALIQNPLWFGKGGWLRVWELDRQVDAQRDTNA
                     QLAARNAALDAEVLDLKQGYDAIEERARSDLGMVKQDEVFFQILQPAPASSGYSPGKR
                     "
     gene            complement(423..1001)
                     /gene="mobA"
                     /locus_tag="VE030_03860"
     CDS             complement(423..1001)
                     /gene="mobA"
                     /locus_tag="VE030_03860"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HZF22585.1"
                     /translation="MILAGGLGRRMGGVDKGLQLLNGRPMAARVLARLAPQVDEVLIN
                     ANQNLERYALLGCRVVPDRLGGYAGPLAGLHCGLSEARHELVATVPCDSPFLPEDLVA
                     RLAAPLQDASVDLAVARTGTQPHPVFCLGRRRLLPHLSAFLQSGGRKIDAWYATLKVV
                     EVAFDDEATAFSNINTAEELRSAETGQRLPGE"
     gene            complement(1079..1900)
                     /locus_tag="VE030_03865"
     CDS             complement(1079..1900)
                     /locus_tag="VE030_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HZF22586.1"
                     /translation="MHRPILTSASRPLTFTVQARNERGETEATSIAGEHPLTLYVDKR
                     EIVTLMTLGGAPEALAIGYLRNQRLIDRLEDIKSVQVDWEANAVAVVTRTGLKDADRK
                     MGKRTVTTGCGQGTVFGELMEEIDKAVLPADTLLGEADFFALLEAVRMRETVYKQAGA
                     VHGCALCKAAEILMFVEDVGRHNAVDAIAGQMWLDRIDGGDKVFYTTGRLTSEMVIKC
                     AQMRIPILVSRSGLTQMGYEIARKVGLTMIGRATGKHYLLFTGEQRFKKAVTGDK"
     gene            2172..2633
                     /locus_tag="VE030_03870"
     CDS             2172..2633
                     /locus_tag="VE030_03870"
                     /inference="COORDINATES: protein motif:HMM:NF023177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3305 domain-containing protein"
                     /protein_id="HZF22587.1"
                     /translation="MKPERLVVGVVMARQPLASKWASFKWEARGVVASDAAAPGQRVL
                     IEDESARQVLFDGFAIELHRDEAEGYYLNLNAPQPKVFVMWRLHGEEPRPHSVTVSYH
                     EAARLLDAGEQVDAVAMPAAMAHWVAGFVEQHYRPEPKKVRKRDQRVGLNR"
     gene            2630..3112
                     /locus_tag="VE030_03875"
     CDS             2630..3112
                     /locus_tag="VE030_03875"
                     /inference="COORDINATES: protein motif:HMM:NF023176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3306 domain-containing protein"
                     /protein_id="HZF22588.1"
                     /translation="MSNDESFLRRWSRRKHESAQTGDAGSGPVESAMPPRLPPVEDLS
                     FESDFKDFMRPEVERETRSAALRKLFMSPHYRATDGLDVYIGDYSSPDPLPAATLAGL
                     VHARGLFADAKPDTPTEPGEPESAVPEGEASPEQAEETASEEQQSNPGDGNAGPPDES
                     "
     gene            3114..4970
                     /locus_tag="VE030_03880"
     CDS             3114..4970
                     /locus_tag="VE030_03880"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZF22589.1"
                     /translation="MSAESKYLLLREQAGWSEEGAAATPKIAALLAAAGLPEADPVPS
                     VSYHSEGKLLIVGDAGPALGWAGELTDTLDVSVLIPGAGGGAELPVDRRFAVMSGRVT
                     RIEGYLGNFSVAWEQLNPIDLEVCTRCNACVRACPEQAIDLLYQVDLDKCKSHRQCVA
                     ACGDIGAIDFRRKKPARSERFDLILNLSDQMLFAMPQPPQGYFAPGSDLAAQARAIKE
                     VVTAIGDFEKPRYFHYDANICAHSRSKLKGCNQCIDICSTQAISADGDRVKVNPHLCM
                     GCGACASVCPSGAMSYAYPKMSFQGEKLRVMLGAYREAGGKDACLLFHNGADGREMIL
                     KLGRRRGLPARVIPVETFHVASLGLDLLLGAVALGAGQVLVVSAGSEAPGYFEALRRQ
                     MGFGEEILRAFGYRGSHFELMVTADLRALEQRIWSLDAAQSPARPAEFRLFDDKRTSL
                     AFAIEHLATHAPMPRTVIPLSTGAPFGEIEVNLETCTMCMACVGACPEAALADGRDRP
                     QLRFVEANCVQCGLCEQACPEDAITLVPRLLVGKEAKAERVLNQDQPFHCIRCGKEFG
                     TQRMLQSMLGKIAGHSMFSAPGALERMKMCGDCRVVDMIEKGGEVSVFDLKP"
     gene            4967..5596
                     /locus_tag="VE030_03885"
     CDS             4967..5596
                     /locus_tag="VE030_03885"
                     /inference="COORDINATES: protein motif:HMM:NF014651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HZF22590.1"
                     /translation="MSAAAAARESEELTPEDAARADLYAMLAHLFHAAPDAVLLAVLA
                     NADDVSAEAPHSAVGEAWRKLQSAAAATDPQALAQEFQDLFVGVGSGEVMAYGSWHLT
                     GFLMEEPLARLRDDLARLGLSRLQSAAEPEDHIAALAEVMRLLVRGGEGIAAAAFEVQ
                     KAFFTRHLWPWYAGFAEQLAKAPNANFYRVVGELAKTFLDTEAELFETY"
     gene            5639..5833
                     /locus_tag="VE030_03890"
     CDS             5639..5833
                     /locus_tag="VE030_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008651781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HZF22591.1"
                     /translation="MSKSKVDRRNFLIALGLGSAGAVAGAVAGEHPQPAQPAANTAQP
                     GSKAYRVSAHIRNYYRTTRV"
     gene            5848..8739
                     /locus_tag="VE030_03895"
     CDS             5848..8739
                     /locus_tag="VE030_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019247523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HZF22592.1"
                     /translation="MQLTRKLPPGAAQASRLSRFVQAVPAGTMDRRAFLKRSGLTVGL
                     GAAVSQLPFNMIGRAKAEAAAAKGGATEVHRTVCTHCSVGCAVDAVVQNGVWIRQEPV
                     FDSPINLGSHCAKGAAVREHGHGERRLKYPMKLVDGKYQRISWEQALNEVGDKLLAIR
                     SASGPDAVFWVGSSKHSNEQAYLLRKFVSFWGSNNCDHQARICHSTTVAGVANTWGYG
                     AMTNSYNDMQNSRAMLFIGSNAAEAHPVAMMHVLHAKEKGSKMIVVDPRFTRTAAKAD
                     HFVRIRPGTDIPFIWGVLYHIFKNGWEDKQFIRDRVYGMEDVHKEVMQWTPDKVRDVT
                     GVDEKDVLNVARIMHENRPGTVVWCMGITQHTVGNANVRALCVLELALGNVGVSGGGA
                     NIFRGHDNVQGATDVGPNPESLPGYYGLAEGAWKHWCAVWGVDYEWVKSRYASQAMME
                     KPGMTVSRWIDGVLEANEHIDQDSNLRALIYWGHAANSQTRGAEMKQAMEKLDLMVII
                     DPYPTAAAVMPDRKDGVYLLPAATQFETSGSCTASNRSLQWREKVIDPLFESRPDQAI
                     MYAFAKKFGFDKELTKSFQLVKGRDGWEEPGTEDILREINRGTWTIGYSGQSPERLKL
                     HMKNMYTFDVVTLRAKGGPCDGETFGLPWPCWGTPEMKHPGTHILYDTSKEVKDGGLC
                     FRANFGVERNGVNLLAGDGSQPKNSDLDGGYPEFDHVLLKKLGWWDDLTAAEKKAAEG
                     KNWKTDLSGGIIRVAIKHGCAPFGNARARAMVWNFPDPVPVHREPLFSPRADLVEKYP
                     SHKDMKTFWRLPTLFRSVQEQNKNIGKAYPIILTSGRLVEYEGGGEETRSNPWLAELQ
                     QEMFAEINPKDANDQGVRNDEYVWVETPTKARIKVKAQITERVAPGVMFMPYHFGGWW
                     EGKDLLEKYPQGAAPFVRGEAVNTATTYGYDSVTMMQETKVTLCRFTRA"
     gene            8768..9382
                     /gene="fdh3B"
                     /locus_tag="VE030_03900"
     CDS             8768..9382
                     /gene="fdh3B"
                     /locus_tag="VE030_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase FDH3 subunit beta"
                     /protein_id="HZF22593.1"
                     /translation="MARMKFICDAERCIECNACVTACKNENEVPWGVNRRRTVTINDG
                     VPGEKTISVACMHCSDAPCMAVCPVDCFYKTEDGVVLHDKDLCIGCGYCFYACPFGAP
                     QFPQTGTFGMRGKMDKCTFCAGGPEADGSEEEFKKYGRNRLSEGKLPLCAEMCSTKAL
                     LGGDGDVLADILRERVVVRGKGAEVWGWGTAYGKPEKKAEEPKS"
     gene            9382..9606
                     /locus_tag="VE030_03905"
     CDS             9382..9606
                     /locus_tag="VE030_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22594.1"
                     /translation="MRVLPAVLLGAALALAGCGEKSQQVSYSNGRYAGKPDTPAWAGD
                     EFKNRADWERAIKARNLRQNEYQRTSGRGG"
     gene            9609..10670
                     /locus_tag="VE030_03910"
     CDS             9609..10670
                     /locus_tag="VE030_03910"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HZF22595.1"
                     /translation="MLTRALLSVWLGLLVTIAGSATVPVAAVRAAENQAAPNRAAEND
                     TQAQRQQKQPLNNAPTWRDVRSGESHTTQVRGRETGVLIQAGGETWRKIRNGPVTLVF
                     GTLLILVPILIGSFYRYSGPMKLHGKRTGRMIERFNKWERIVHWFTAVSFVVLAVTGI
                     LMLFGKHFLLPVIGYSAFSWFGAFSKNLHNFVGPLFIFCVACIFVTFVRDNFFRRIDI
                     QWLLKMPQVLTGKAHVPSGRFNGAEKGWFWGGVALLGLIVSASGLVLDFPNFDQTRNT
                     MQIANIVHGVGALLFVLASFGHIYMGTIGTEGAYQAMKTGMVDETWAREHHQLWYEEV
                     KAGKRGGGDTRRAELLGGS"
     gene            10672..10893
                     /locus_tag="VE030_03915"
     CDS             10672..10893
                     /locus_tag="VE030_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22596.1"
                     /translation="MIEGKMSLAGLAGMLLLATHAAWGKLPPPTEAEAQAKKAAADKK
                     VKDEEAAKQALAKAQDRVAARYKKSGKRK"
     gene            11033..12676
                     /locus_tag="VE030_03920"
     CDS             11033..12676
                     /locus_tag="VE030_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HZF22597.1"
                     /translation="MRGTPKGRQVDPAALAEVQALLGETPRRRDLLIEHLHGIQDRYR
                     QLSSAHLAALAQEMKLSQAEVFEVASFYHHFDIVKEGQAAAPPLTVRVCDSLSCELAG
                     AKDLLARLPAILGRELRVIPAPCVGRCETAPVVVVGRNPLPNATPEAVKVAVSGAKTS
                     CPPTRYTGYQAYRKAGGYATLAKCLNGKLDREDVFKTMEHSGLRGLGGAGFPAGRKWR
                     IVWAEPAPRLLAVNIDEGEPGTFKDRWYLERDPHRFLDGMLIAGWATGCAEIYIYLRD
                     EYAGCREILEKELAALKKAPPCALPPIHLRRGAGAYICGEESAMIESIEGKRGMPRLR
                     PPYVAQVGLFGRPTLEHNMETLYWVRDILEKGAEWFASQGRHGRKGLRSFSVSGRVAR
                     PGVHLAPAGITVKELIAEHCGGMAPGHAFYAYLPGGASGGILPASMGDIPLDFDTLNE
                     YGCFIGSAAVVILSDRDRASDAARNLMAFFSDESCGQCTPCRVGTVKALQLMRKDDWD
                     KDLLEELSQAMADASICGLGQAAPNPVRCVIKYFSHELK"
     gene            12843..15644
                     /gene="fdhF"
                     /locus_tag="VE030_03925"
     CDS             12843..15644
                     /gene="fdhF"
                     /locus_tag="VE030_03925"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HZF22598.1"
                     /translation="MNQRSEMIAFRLDGVEIHAAPGETILQAAERYGVDIPRLCYKPG
                     YRPDGNCRACMVEIKGERVLAPSCCRAPKAGMEVESKNARAVAAQKMILELLLSDMPE
                     QKYSRESELDLWARNLKVGKPRFAGRHQPKADLSHPGIAVNLDACIQCTRCVRACREE
                     QVNDVIGYAFRGDESQIVFDLDDAMGDSTCVACGECVQACPTGALMPAHGVGMKVADR
                     KVDSVCPYCGVGCQLTYHVKGDRILQVTGRDGPANHGRLCVKGRYGFDYVHNRQRLTK
                     PLIRKSGIPKSADFVVDPDNFLDVFREASWDEALDLAAGRLRGIRDSHGKRALAGFGS
                     AKGSNEEAYLFQKLVRTGFGSNNVDHCTRLCHASSVAALLEGIGSGAVSNQVSDCMNA
                     EVVIIIGANPTMNHPVAATWFKNAAKKGVKLIVADPRRSDLARHATHFLQFKPDTDVA
                     LLNAMMHVIVYEGLVNEAFIASRTIGYDDLKKNVEGYSPAQMAPVCGVPAQIIREVAR
                     LYASSKASMIFWGMGISQHVHGTDNARCLIALALMTGQVGRPGTGLHPLRGQNNVQGA
                     SDAGLIPMMFPDYHRVDNVEFRGQYEKLWGVALDPRPGLTVVEIMHAARKREIRGMYV
                     MGENPAMSDPDLDHAREALAALDWLVVQDIFLTETAYLADIVLPASAWPEKTGTVTNT
                     DRMVQLGRRALDMPGDARQDLWIIQEMARRFGLDWRYQGPESGVAEVFDELRRAMQSI
                     AGITWERLERESSVTYPCEHEGDPGEPVVFTDHFPTPTGRGKFVPADVIPAAERPDAE
                     YPLVLITGRQLEHWHTGAMTRRASVLDAIEPDPVASFHPLDLARLAIRPGDVVTVESR
                     RGRIALYARADDGTPAGAVFIPFCFYEAAANLLTNPALDPVGKIPEFKYCAVKVTRGG
                     KMAAQMSFGGGKPVAAVAG"
     gene            15670..16452
                     /locus_tag="VE030_03930"
     CDS             15670..16452
                     /locus_tag="VE030_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZF22599.1"
                     /translation="MGKRTYEYIVRMERIRMAYGNVIALNDVSLTVGRDEIVGLIGDN
                     GAGKSTLIKIMTGVVRPTGGELYIRDERIDLSHYSVRQAHNRRIETVYQEKSLGEKQP
                     LWRNFFVGRQITNRFGFIDIKKQKAITNDVLKNQIGFRGAGVDADSPVQKLSGGERQG
                     IAIGRAMYFDADLVVLDEPTVALAIKEVQKVLDFVRSIKQRGRACVYIEHNLAHVHEL
                     ADRLVILDRGEISKIFHKGEVTLTELTRFLLDLQNDNAEVAV"
     gene            16452..17447
                     /locus_tag="VE030_03935"
     CDS             16452..17447
                     /locus_tag="VE030_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZF22600.1"
                     /translation="MQRISMSRVEGLPIIVVFVALIGCFIVAAPEVFLSLPIYFSFLT
                     TVPPVLVLALGLTFVIAAGEIDLSFPSVIAFSGYLLAMLYRDHDMIWLAVLAAIAGGA
                     LIGLINGLIVAYIGVPSIITTLATQFFWGGITTVISGGTSYSLRTVDETSMWVVFVGK
                     IGDFPAQALWAAGLAVLLWFIMNRHRFGEHVLFIGDNANVARVVGVNVARERVKLFTL
                     MGTLAGFAAILLTLENRNFFNTQGQGYLLIALASVFIGGTSIFGGQATIVGTFFGAFI
                     ISMIEAGLTATGIQGFWVRAVVGLVFLAAVIFHLTMEQPQRRAKLARVFGIKPGK"
     gene            17574..18560
                     /locus_tag="VE030_03940"
     CDS             17574..18560
                     /locus_tag="VE030_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZF22601.1"
                     /translation="MKGKILAATAAAVILAALYVPSQAAEPLGKGMTMYMQMGGNAGD
                     GATWARTNGALAAADAFGIKVIEQYSAWQPEKMLEHFRQALAAKPDCIGIMGHPGNNA
                     FEPLVKQAEDRGILVTVGNSPLTKIQKMYGSKGTGYAGVELYAGGAITASKMLEAGLK
                     SGDKALVYGLLSEAERGQSDKGLKDTLEKAGVKVDYLEISAEVNSDSSLAVPVLTAYI
                     AKNPTVKGIGTQHGGVTSVIPKALQAAGKKPGDVIVGGIDLSPATIDGLQKGWITVTL
                     DQQLYLQGFLPVMQCVLSKKYGFAGLSINTGADVVTPNTIKTLVPLIEKGIR"
     gene            18628..19455
                     /locus_tag="VE030_03945"
     CDS             18628..19455
                     /locus_tag="VE030_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZF22602.1"
                     /translation="MKTILITGAAGDVGTHLRRELAGKYNLRLSDIRPVKKLAAGEKS
                     MPGDIARMADMRRVTKDADAIVHLGGFAVEGPWDMILRANIAGCYNTFEAARENGVKR
                     ILFASSNHATGFYRRDQKIDHRVYPKPDSRYGLSKVFGEQLGSLYADKYGMQVFCMRI
                     GNVNLAPADKRRLSIWISPRDFAQLVIIGVEHPDIRFEIVYGVSGNQRSWYDNSNAER
                     LGYRPQDDAEAYAAAVLAKEKPGADPRTEMFQGGTFVLAEIGGDPSRALPKIKKKKR"
     gene            19452..19643
                     /locus_tag="VE030_03950"
     CDS             19452..19643
                     /locus_tag="VE030_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22603.1"
                     /translation="MSEVPKVPAPAGSCDTHMHFYDRAVPSAPGGPPLPGHYTVTMYR
                     ELQKQLGLERVIVVQPNAY"
     gene            19656..20282
                     /locus_tag="VE030_03955"
     CDS             19656..20282
                     /locus_tag="VE030_03955"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZF22604.1"
                     /translation="MTLSAIAELGSRAKGVAVVGPDVSDAEIERLTAAGVCAQRIFAL
                     PGGAVGFDRMDQIMARVHRYGWHANIQLNGRDLPRYEAQLRRLPGHFVIDHVGKYIDP
                     VPPEHEAFQALRRLLDTGRCWVKLSAPYESSKSGAPAYDDVARLARELVKHAPERMLW
                     ASNWPHPYRSPVPSSAAMLDLLADWAPDDATRRQILVDNPAALYGFPR"
     gene            20279..21328
                     /locus_tag="VE030_03960"
     CDS             20279..21328
                     /locus_tag="VE030_03960"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZF22605.1"
                     /translation="MKKARIAIVGLGMAVVPHAKSLIELSDRVEVAYAFSPSPARRAK
                     FAETFAFPQCQSMEAVLNDRSIDAVAVLTPANTHLDLVRRCAVAGKHVLVEKPLEITT
                     ARAEEMVADCRAAGVTLGVVLQHRHKPAVKRLASALASGELGMLVGASAVVRLWRPQS
                     YYDEPGRGTRARDGGGVLITQAIHTLDVLLSLAGDPQEVMSYAATTPVHRMECEDLVC
                     AAVHWGSGALGVIEATTAAHPGAAERIELTGTRGTASLAGTALELRWHDGGSETMAAD
                     GSAGGAGADPMAFPHDYHRSVWRDFIDAVQSGGEPRVNGAEALRVHRLIDALLASAAT
                     GKAVRRNLAAGGGTS"
     gene            21325..22350
                     /locus_tag="VE030_03965"
     CDS             21325..22350
                     /locus_tag="VE030_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006754171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HZF22606.1"
                     /translation="MNSDSRRRFLASASVATLALAGGMRRARAQSAVTLRISSSLPAD
                     QNAAHYPWYQRFAANLQGATGNGIAIEYFPNSQLGKEADVVQQVKVGSIDMMITGSSI
                     WATVAPEFGMLDLGYVFDSYAHVAKALDGGVGERLARMLFDRTGCTVLGWASHFGARS
                     VYAKNPIKSLAGVKGVKLRVLPTTAFIETFKLMGAIPTPIPFNELYTALQTGVVDGFE
                     HDAATVVASKLYEVSKYCWLTEHLFSPMIAVMGRRGLEKIRADVRPVLFKAAAEATAY
                     QRAHAAEKGRAAVEELKRLGITFQPMAKTEREFVRKEMETKLWAAFAAQYPATKPLFE
                     AIAAARA"
     gene            22409..24289
                     /locus_tag="VE030_03970"
     CDS             22409..24289
                     /locus_tag="VE030_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HZF22607.1"
                     /translation="MNPHDARPYAKGFASAGGAHRLLAGLLAVIEYFAAFVLAVDVLM
                     VFASVIWRYFLHDPLNWAEEIARALMVTQVFLGAATAVGRAQHVGIDSFRTLFPLSWR
                     PAMIQLCDWIIVAVSVALLFSSAAMLIDSRDQTTPIGLPQWIYIWPVVAGSLLMIVFG
                     TAAALDGRWKHVWGTLAATLGLALAVWGWNTTVPTHAVTPAFLLVAGFVASLVIGVPI
                     AFALAFSSLLYFLVDPSLPMLVYSQQVLSGMDHFVLLAIPFFVLAGLIMEANGMSARL
                     IELLLRMMGRLRGGLNLIMIAATAFFSGVSGSKLADIAAVGGIIMPAVRRTRQDPNDA
                     AGLLASTAVMAETIPPCINMIILGFVANISIGGLFLAGLVPAGVLAMALAAVAVYCGS
                     RINPNDAFEVQTPLIRLIGGAMVGLVMVLMIGKGVVSGVATSTEVSAFAVIYALVVGG
                     LAFRELSVKSIVGLFVRSASMAGGILFIVAAASSVSFALTIQQIPQYLSDTMVALGHQ
                     HGPQAFVVVSALLMIVFGAILEGAPALIIFGPLLAPIAQQLGVHPLHFGTVMVIAMGL
                     GLFSPPIGLGLFATCAITGTQMKDVAGPMLKYLAVLFAGLMLLVFVPAFSLWLPFHMG
                     MK"
     gene            24305..24649
                     /locus_tag="VE030_03975"
     CDS             24305..24649
                     /locus_tag="VE030_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22608.1"
                     /translation="MAPMSWHGWLIFAMVALPLIGFLLWIFGQFLRARREETETVLRK
                     GLPAQAEIIACAPGLRGATVRYRFLAPGWEHPIVVTQRLPPGSKFAVGDKVAIRYLPR
                     HPHIAVIVPKAK"
BASE COUNT         4627 a         7633 c         8233 g         4554 t
ORIGIN      
        1 ctgcagatca agaccggctc gctgtcgcgt tccgaccggc tggcgaagta caaccagttg
       61 ctgcgcatcg aggaagatct tggcgattcg gcgagctacg cgggccgcga cgccttctat
      121 cagatcaatt agccgatgcg cttcctcgcc tttgcactgg tcgtgcttat cgcgctgata
      181 cagaacccgc tgtggttcgg caaaggcggt tggctgcgcg tctgggaact cgaccgccag
      241 gttgacgcgc agcgcgacac gaatgcgcag cttgccgcgc gcaatgccgc gctcgatgcc
      301 gaagtcctgg atctaaagca gggctacgac gcgatcgagg agcgcgcgcg gtccgatctc
      361 ggcatggtca agcaggatga ggtgtttttc cagatcctgc agcccgcgcc cgcgtcgtcg
      421 ggctattccc ccggcaaacg ctgaccggtc tcggcggagc gcaattcctc cgcggtgttg
      481 atgttgctga acgccgtggc ctcgtcgtcg aacgctactt ccacaacttt cagcgtcgcg
      541 taccacgcgt cgatcttgcg gccgccggat tgcagaaatg cggacaggtg gggcagcagg
      601 cggcgcctgc ccaggcagaa caccgggtgc ggctgagttc cggtcctggc caccgccagg
      661 tccacgctcg cgtcctgcag cggtgccgcg agccgcgcga ccaagtcttc gggcaggaaa
      721 ggcgaatcgc agggaaccgt ggccaccagt tcgtggcgcg cctccgatag tccgcaatgc
      781 agtccggcca acggccccgc atatccgccg agccggtccg gtacgacgcg gcagccgagc
      841 agcgcataac gctccaggtt ctggttggcg ttgatcagga cctcatcgac ttgcggcgcc
      901 agccgcgcca gcacccgcgc tgccatcggc cggccattca gcaactgcag tcccttgtcc
      961 acgccgccca tgcggcggcc aagccccccg gccagaatca cgcccgtcac cgccggcgtc
     1021 gcgccggggt gaggagtgac gagtgacgag tgatgcgaag tcccctggtt tttccctgtc
     1081 acttgtcacc tgtcactgct ttcttgaacc gctgttcgcc ggtgaagagc agataatgct
     1141 tcccggtcgc ccggccgatc atcgtgagac cgaccttccg ggctatttcg tagcccatct
     1201 gcgtgagtcc ggagcgcgac accagaatcg gtatgcgcat ttgcgcgcac ttgatcacca
     1261 tttccgaagt aagccgtccg gtggtgtaga acaccttgtc gccgccgtcg atgcggtcca
     1321 gccacatctg cccggcaatc gcgtccaccg cgttgtggcg gccgacgtcc tcgacgaaca
     1381 tcagaatttc cgcggccttg cacaacgcgc agccgtgcac cgcgccggcc tgcttgtaga
     1441 cggtttcgcg catgcgcacg gcctcgagca gcgcgaaaaa atccgcttcg ccgagcagcg
     1501 tatccgcggg cagcacggcc ttgtcgattt cctccatcag ctcgccgaac accgtaccct
     1561 gtccgcagcc ggtggtcacc gtgcgcttgc ccatcttgcg atccgcatcc ttgaggcccg
     1621 tgcgggtcac gacggcgacc gcattggctt cccaatccac ctgcaccgac ttgatatcct
     1681 cgaggcgatc gatcaggcgc tggttgcgca gatagccgat cgccagcgcc tcgggcgccc
     1741 cgccgagcgt catcagggtg acgatctcgc gcttgtccac gtaaagcgtc agggggtgct
     1801 cgccggcgat cgacgttgct tccgtttcgc cgcgttcgtt gcgggcttgc accgtgaacg
     1861 tgagcgggcg gcttgccgaa gtcaggattg gtcggtgcat gtgggcgcgg gtgttgatga
     1921 ctggatcact ggtgcgatgc aaaatcgccg atggtcgaaa ttatcgcaaa tccccgtctt
     1981 tgacataccg gccgggatat ttccatgttc ctggtagggt gagagcagta atcaggggcc
     2041 ggggtcgtca aaaatcggga ctaagatagt ctcgtggccg gtattgtgca tgccgtgctc
     2101 cagggtctct ataattcttt ccggggacca gagaaactat ttttctctgt ggggtcttaa
     2161 cggtggaaac gatgaaacct gaacgtcttg tcgtgggcgt cgtgatggcg cgccagccac
     2221 tggccagcaa gtgggcttcc ttcaagtggg aagcgcgtgg tgtggtggcc agtgatgcgg
     2281 cggcgcccgg gcagcgggtc ctgatcgagg atgagtccgc gcgccaggtg ctgttcgacg
     2341 gtttcgcgat cgaactgcat cgcgacgagg cggaaggcta ttatctcaac ctgaacgcgc
     2401 cgcagccgaa agtattcgtg atgtggcgct tgcacggcga agagccgcgg ccgcattcgg
     2461 taacggtaag ctaccacgaa gcggcgcgat tgctggacgc gggcgagcag gtggacgccg
     2521 tggcgatgcc ggccgcaatg gcccattggg tggcgggttt cgtggagcag cattaccgcc
     2581 cggaaccgaa gaaggtacgc aagcgcgacc agcgcgtggg cctgaaccgg tgagcaacga
     2641 cgaatctttc ctgcgccggt ggtcgcggcg caagcacgaa tccgcgcaga ccggcgacgc
     2701 cggatcgggg ccggtcgaat ccgcgatgcc gccgcgactg ccgcctgtcg aagacctgag
     2761 ttttgaatcc gacttcaagg acttcatgcg tcccgaagtg gagcgcgaga cgcgcagcgc
     2821 ggcgctgagg aagcttttca tgagcccgca ttaccgggcc accgacgggc tggacgttta
     2881 catcggcgat tattcatctc ccgatccgct gcctgccgca acgcttgccg ggctggtgca
     2941 cgcccgtggg ctattcgcag atgcaaagcc cgacacgccg acggaacccg gagaacccga
     3001 atccgctgtc ccggaaggcg aggcatctcc ggagcaggca gaggagacag cgtctgagga
     3061 acagcagtcg aatccggggg acggaaacgc cggacctccg gatgagagtt gacgtgtctg
     3121 ccgaatcgaa atatctcctg ctgcgcgagc aggccggatg gtctgaagaa ggtgctgcgg
     3181 caacgcccaa gattgcggcg ctgctggccg cggcaggact acccgaagcc gatcccgttc
     3241 caagcgtctc gtatcattcc gagggcaaac ttctgatcgt cggcgacgcc gggccggcgc
     3301 tgggctgggc tggcgaattg accgacacgc tcgatgtatc ggtcctgatt cccggcgccg
     3361 gtggcggcgc agaattgccc gtagatcgcc gcttcgcggt gatgtcgggc agagtcacgc
     3421 ggatcgaggg ctatctcggc aacttcagcg tcgcctggga acagctcaac ccgatcgacc
     3481 tcgaggtctg tacccgctgc aatgcctgtg tgcgcgcctg tccggaacag gcgatcgacc
     3541 ttctgtatca ggtcgatctc gacaagtgca agagccaccg gcagtgcgtg gcggcctgcg
     3601 gcgatatcgg ggcgatcgat ttcaggcgca agaagcccgc gcgcagcgag cggttcgatc
     3661 tgatcctgaa tctctccgat cagatgctgt tcgcgatgcc gcagccgccg cagggttatt
     3721 tcgcgccggg aagcgatctg gcggcgcagg ccagggccat caaggaagtc gtcaccgcga
     3781 tcggggattt cgagaagccg cgctatttcc attacgacgc gaatatctgc gcgcacagcc
     3841 gctcgaaact caagggctgc aaccagtgca tcgacatctg ctcgacgcag gcgatttcag
     3901 ccgatggcga tcgcgtcaag gtgaatccgc atttgtgcat gggctgcggc gcctgtgcca
     3961 gcgtctgccc atccggggcg atgagttatg cctacccgaa aatgtccttc cagggtgaaa
     4021 agctgcgggt gatgctcggc gcctatcgcg aggccggcgg caaggacgcc tgcctgctgt
     4081 tccacaacgg agcggacggg cgcgagatga ttctgaagct tggccggcgt cgcggtttgc
     4141 ctgcgcgggt gatcccggtc gaaactttcc acgttgcctc gcttgggctc gatctgctgc
     4201 tcggtgcggt cgcgctcggg gccggtcagg tcctcgtggt gtctgcgggc agcgaggcgc
     4261 ccggctattt cgaggcgctg cgccggcaga tgggctttgg cgaggagatc ctgcgggcct
     4321 tcggttaccg gggcagccat ttcgagctca tggtgaccgc agacctgcgc gcgctggagc
     4381 agcgaatctg gagtctcgat gcggcgcaaa gtccggcgcg gcctgccgag ttccgcttgt
     4441 tcgatgacaa gcgtacctcg ctggctttcg cgatcgagca tctggcgacc cacgcgccca
     4501 tgccgcggac ggtgattccg ctgtcgactg gcgctccatt cggtgagatc gaggtcaatc
     4561 tcgaaacctg caccatgtgc atggcctgcg ttggggcctg tccggaagcc gcgcttgccg
     4621 acggccgcga ccggccgcaa ctgcgcttcg tcgaggccaa ctgcgtgcag tgcggacttt
     4681 gcgaacaggc ctgtccggag gatgccatta ccctggtgcc gcgattgctg gtgggcaagg
     4741 aggccaaggc agagcgggtg ctgaaccagg accagccttt tcactgcatc cgctgcggca
     4801 aggaattcgg cacccaacgg atgctacaga gcatgctcgg caagatcgcc ggccattcga
     4861 tgttttccgc acccggggcg ctcgaacgca tgaaaatgtg cggggactgc cgggtggtgg
     4921 acatgatcga aaaaggcggc gaggtcagcg tgttcgatct caaaccatga gtgccgccgc
     4981 cgcagcgcgc gagtccgagg aactgacccc tgaggacgcg gcccgggcgg acctgtatgc
     5041 gatgctggcg catctttttc acgccgcgcc ggatgccgtt ctgcttgcgg tgctggcaaa
     5101 cgcggacgat gtctcagccg aagcgccgca cagcgccgtg ggcgaggcct ggcgcaagct
     5161 gcaatcggcg gctgcggcca cggacccgca ggctctggcg caggagtttc aggacctgtt
     5221 cgtgggtgtc ggcagcggcg aggtaatggc ctacggctcc tggcatctca ccggttttct
     5281 gatggaggag ccgctggcca gactgcgcga cgatctcgct cgacttggac tttctcgctt
     5341 acaatctgcg gcagagccgg aggaccacat cgccgcgctt gccgaggtga tgcggctgct
     5401 ggtgcggggc ggcgagggaa ttgcggcggc cgcattcgag gtacagaaag cttttttcac
     5461 gcgccacctg tggccctggt atgcaggctt cgccgaacaa ctggcaaagg cgccgaacgc
     5521 caatttttac cgcgtcgttg gggagctggc caagacgttt ctggacaccg aggcggagtt
     5581 gttcgagact tactagagcc gaagactgaa aacagcggct acacgaagga ggagaccgat
     5641 gagcaagtca aaggtcgatc gcaggaactt ccttatcgcc ttggggctgg gcagcgccgg
     5701 cgcagtagcc ggtgcagtgg cgggagagca tccccagccg gcgcagcctg ccgcaaacac
     5761 ggcacagccc gggtccaagg cctaccgggt ttccgcccat atccgcaatt actaccggac
     5821 cacccgcgtt taggcgcagg agggtttatg caactcacca gaaaattacc cccaggcgcg
     5881 gcgcaggcgt cgcgtctatc ccggtttgtc caggcggtac ctgcaggtac gatggaccgc
     5941 cgcgcttttc tcaaacgctc cgggctgacc gtcggcctgg gcgctgccgt cagccagttg
     6001 ccgttcaaca tgatcggcag ggcgaaagcc gaagcggcgg ccgccaaggg cggcgcaacc
     6061 gaagtgcatc gcaccgtctg cacccattgc tcggtcggct gcgccgtgga cgcggtagtg
     6121 cagaacggag tttggatacg gcaggagccg gtgttcgatt cgccgatcaa cctcggctcg
     6181 cactgcgcca agggcgcggc ggtacgcgaa cacggtcacg gcgagcggcg cctgaaatac
     6241 ccgatgaagc tggtcgacgg caagtatcag cggatttcct gggaacaggc cctcaatgaa
     6301 gtcggcgaca agctcctggc aattcgcagc gccagcggcc cggacgccgt gttctgggtg
     6361 ggttcctcga agcacagcaa cgagcaggcc tacctgctgc gcaagttcgt ctctttctgg
     6421 ggctcgaaca actgcgatca ccaggcgcgc atctgccact cgaccacggt ggccggggtg
     6481 gcaaacacct ggggttacgg cgcgatgacc aactcctaca acgacatgca gaactccagg
     6541 gcgatgttgt tcatcggcag caacgccgcg gaagcgcatc cggtggcgat gatgcacgtg
     6601 ctgcacgcca aggagaaggg ttcgaaaatg atcgtggtgg atccgcgctt cacccgcacc
     6661 gccgccaagg ccgaccattt cgtgcgcata cgcccgggta ccgatattcc gttcatctgg
     6721 ggcgtgctct atcacatctt caagaacggc tgggaagaca agcagttcat ccgtgaccgc
     6781 gtctacggca tggaagacgt gcacaaggaa gtcatgcaat ggaccccgga caaggtcagg
     6841 gacgtgaccg gcgtggacga aaaagacgtg cttaacgtcg ccaggatcat gcacgagaac
     6901 cggccgggta ccgtggtttg gtgcatgggc atcacccagc acacggtggg caacgccaat
     6961 gtgcgcgcgc tttgcgttct cgaactggcg ttgggcaacg tcggcgtctc cggcggcggg
     7021 gcgaacatat tccgcggtca cgacaacgtc cagggcgcga ccgacgtcgg cccgaacccg
     7081 gaatcgctgc ccggttatta cggccttgcc gagggcgcct ggaagcactg gtgcgcggtg
     7141 tggggcgtgg attacgaatg ggtgaagagc cgctatgcgt cccaggccat gatggaaaag
     7201 ccgggaatga cggtgtcgcg ctggatcgac ggcgtgctgg aagccaacga acacatcgac
     7261 caggactcga acctgcgcgc gctgatttac tggggccacg ccgccaacag ccagacccgc
     7321 ggcgcggaaa tgaagcaggc gatggagaaa ctcgacctga tggtgatcat cgatccgtac
     7381 ccgaccgcgg cggcggtgat gccggatcgc aaggacggag tgtacctgct gccggcggcg
     7441 acgcagttcg agacctcggg ttcctgtact gcctcgaacc gctcgctgca atggcgggag
     7501 aaagtgatcg atccgctgtt cgagtccagg cccgatcaag cgatcatgta tgcgttcgcg
     7561 aaaaaattcg gctttgacaa ggaactcacc aaaagcttcc agctcgtcaa aggcagggac
     7621 ggctgggaag agcctgggac cgaggacatc ctccgggaga tcaaccgcgg cacctggacc
     7681 attggataca gcggccagtc gccggaacgg ctcaagctgc acatgaagaa catgtacacc
     7741 ttcgacgtgg taacgctgcg ggcgaagggc gggccatgcg atggcgaaac ctttggcctg
     7801 ccgtggccgt gctggggcac gccggagatg aaacaccccg gcacccatat tctgtacgac
     7861 acttccaagg aggtgaagga cggcggcctt tgcttccgcg ccaatttcgg cgtcgagcgc
     7921 aacggcgtca acctgcttgc cggcgacggt tcgcagccga agaattccga cctcgatggc
     7981 ggttatccgg agttcgacca cgtgctgctg aagaaactgg gctggtggga tgatctcact
     8041 gccgcggaaa aaaaggccgc cgaaggcaag aactggaaga ccgacctgtc gggcggaatc
     8101 atccgcgtgg cgatcaagca tggctgcgcg cctttcggaa atgccagggc gcgcgcaatg
     8161 gtgtggaatt tccccgaccc ggtcccggtg caccgtgagc cattgttctc accgcgcgcc
     8221 gatctggtcg aaaagtaccc gagccacaag gacatgaaga cattctggcg cctgccgacg
     8281 ctgttccggt cggtgcagga gcagaacaag aacatcggca aggcctatcc gatcattctg
     8341 accagcgggc gtctggtcga atacgaaggc ggcggcgagg aaacgcgctc caatccctgg
     8401 ctggcggagc tgcagcagga aatgttcgcg gagatcaatc cgaaggacgc caacgaccag
     8461 ggcgttcgca acgacgagta cgtctgggtc gaaaccccca ccaaggcacg catcaaggtc
     8521 aaggcgcaga tcaccgaacg ggtggcgccc ggcgtgatgt tcatgcccta tcactttggc
     8581 ggctggtggg agggcaagga cctgctggaa aagtatcccc agggcgcagc gcctttcgtg
     8641 cgcggtgagg cggtgaacac cgcgacgacc tatggctacg actcggtcac catgatgcag
     8701 gaaaccaagg tgaccctttg ccggttcact cgcgcctagt gcggcgagcg cgagaaggag
     8761 agatcaaatg gcccgaatga aattcatctg tgatgcggag cgctgcatcg agtgcaacgc
     8821 ctgcgtcacg gcgtgcaaga acgagaacga ggtgccgtgg ggcgtcaacc gccggcgcac
     8881 ggttacgatc aatgacggcg tacccggcga gaagacgatt tcggttgcct gcatgcactg
     8941 ctcggacgcg ccgtgcatgg cggtgtgccc ggtggactgc ttctacaaga ccgaggacgg
     9001 cgtggtgctg cacgacaagg acctgtgtat cggctgcggt tactgtttct acgcctgtcc
     9061 gttcggcgcg ccgcagttcc cgcagaccgg cacgttcggc atgcgtggca aaatggacaa
     9121 gtgcaccttc tgcgccggcg gccccgaggc cgacggctca gaagaggaat tcaagaagta
     9181 cggccgcaat cggctgtccg agggcaagct gccgctgtgc gcggaaatgt gctccaccaa
     9241 ggcgctgctg ggaggcgatg gcgacgtgct cgcggacata ctgcgcgaaa gggtcgtcgt
     9301 gcgcggcaag ggcgccgaag tgtggggctg gggcaccgcc tacggcaagc cggagaagaa
     9361 agccgaggag ccgaaatcgt gatgcgcgtg ttgccggccg tgttgcttgg cgccgcattg
     9421 gcgctggcag gatgcggcga gaaatcacaa caggtttcct acagcaatgg ccgctacgcg
     9481 ggcaagccgg atacgcccgc ctgggccggg gatgaattca agaaccgcgc cgattgggag
     9541 cgcgcgatca aggcccgcaa cctgcgccag aacgaatacc agcgcacctc gggtcgcggc
     9601 ggctgaccat gctgacacgc gcgctgctga gtgtgtggct ggggttgctg gtgacgatag
     9661 ccggttcggc caccgtaccg gtcgccgccg ttcgcgcggc ggagaatcag gccgccccga
     9721 accgggccgc ggaaaacgac acccaggccc agcgccagca gaaacagccg ttgaacaatg
     9781 cgccgacctg gcgcgacgtg cgctccggcg aatcgcacac cacgcaggtg cgcggacgcg
     9841 agaccggcgt gctgatccag gcgggcggcg agacctggcg caagatccgc aacggcccgg
     9901 tcacgctggt tttcggcacc ctgctgatcc tggtgcccat cctgatcggg tcgttttacc
     9961 gctacagcgg accgatgaaa ctgcacggca aacgcaccgg acggatgatc gagcgcttca
    10021 acaagtggga gcgcatcgtg cattggttca cggcggtgag cttcgtggtg ctggcggtca
    10081 ccggcatcct gatgcttttc ggcaagcatt ttcttttgcc ggtgatcgga tacagcgcct
    10141 tctcgtggtt cggcgcgttc tcgaagaacc tgcacaattt tgtcggtccc ctgttcatct
    10201 tttgcgtggc ctgcattttc gtgacttttg tgcgcgacaa ttttttccgc aggatcgata
    10261 ttcagtggtt gttgaaaatg ccgcaggtgc tgaccggcaa ggcgcacgtt ccctcggggc
    10321 ggttcaatgg cgcggaaaaa ggctggtttt ggggcggagt ggcactgctc ggtttgatcg
    10381 tgagcgcctc cggcctggtg ctggacttcc ccaattttga ccagacacgc aacacgatgc
    10441 agatcgccaa tatcgtgcat ggcgtcggcg cgcttctgtt cgtgctggct tcgttcggcc
    10501 acatttacat gggaaccatc ggtactgaag gcgcctacca ggcgatgaaa accgggatgg
    10561 tggacgaaac ctgggccagg gagcatcacc agctctggta cgaggaagtc aaggcgggaa
    10621 aacgcggcgg cggtgacacg cgacgagccg aactgctggg aggtagctga cgtgattgaa
    10681 ggaaagatgt cgctcgccgg cctggccggc atgctgctgc tggcaaccca tgcagcgtgg
    10741 gggaagctgc cgccgccgac cgaagccgaa gcccaggcga agaaggctgc cgcagacaag
    10801 aaagtaaaag acgaggaagc ggccaagcag gcgctcgcca aagcacagga tcgcgttgcg
    10861 gcgcgttaca agaagtctgg gaagcgcaag tagttctccc ccgcgggtgg ccgctgtagc
    10921 gctgtcccgc ctgccatcct gcaacagtct gcggctgtgt caacaggagt agtccgattt
    10981 gccaaagcag gctgcaaacg actctggaac tgcccgcgga gatagccgca ggatgcgcgg
    11041 tacccccaaa gggcggcagg tcgatcccgc ggcgctcgct gaagtacagg cgcttctcgg
    11101 tgagacgccg cgccggcgcg acctgttgat cgagcacctg cacgggattc aggaccgcta
    11161 ccgccagctc tcgtcggcgc acctggctgc gcttgcccag gaaatgaaac tgtcgcaggc
    11221 ggaggttttc gaggtcgcga gcttctatca ccacttcgac atcgtcaagg aaggacaggc
    11281 cgcggcgccg ccactcacgg ttcgcgtgtg cgattcgctg tcctgcgagc tggctggtgc
    11341 gaaggatctg ctggcgcgcc tgcccgcgat cctgggccgg gaactccggg tgatcccagc
    11401 gccctgcgtc gggcgctgcg agacggcgcc ggtggtggtg gtcggacgga accctttgcc
    11461 gaacgcgacg ccggaggcgg ttaaagtcgc cgtgagcggc gcgaagacca gttgcccgcc
    11521 gacgcgctac accggttatc aggcgtatcg gaaggccggc ggctacgcca cgctggcaaa
    11581 atgcctgaac ggaaagctgg accgcgagga cgtattcaag accatggagc attcgggcct
    11641 gcgcggactg ggcggcgccg gctttccggc cggacgcaaa tggcgcatcg tctgggccga
    11701 gcccgcaccg cggctgctgg cggtcaacat cgacgaaggc gaaccgggca cattcaagga
    11761 ccgctggtat ctcgagcgcg acccacaccg gttcctggac ggcatgctca tcgcgggctg
    11821 ggccacaggc tgtgcagaaa tttacattta tttgcgcgac gaatacgccg gctgccgcga
    11881 aatcctggaa aaggaactcg cggcgctcaa aaaggcgccg ccctgcgcgc tgccaccgat
    11941 ccatctgcgc cgtggggccg gcgcctacat ctgcggtgaa gagtcggcga tgatcgaatc
    12001 gatcgaaggc aagcgcggca tgccgcggct gcgcccgccg tacgtggcgc aagtcggcct
    12061 gttcgggcgg ccgacgctcg agcacaacat ggaaacactt tattgggtgc gcgacatcct
    12121 ggaaaagggc gcggagtggt tcgcctcgca gggacgtcat ggccgcaagg gcctgcgctc
    12181 gttctcggtg agcggacgcg tggcaaggcc gggcgtgcat ctggcgccgg ccgggattac
    12241 agttaaggag ctgatcgcgg aacactgcgg cggcatggct ccgggacatg cgttctacgc
    12301 ctacctgccg ggcggtgcgt cgggcggcat cctgccagcc tcgatgggcg acataccgct
    12361 ggacttcgac acgctgaacg aatacggctg cttcatcggc tctgcggcgg tggtgatcct
    12421 gtcggatcgg gatcgcgcgt ccgacgccgc gcgcaacctc atggcgttct tctccgacga
    12481 atcctgcggc cagtgcacgc cctgccgcgt cggcacagtc aaggcgctgc agttgatgcg
    12541 gaaggacgac tgggacaagg atctgctgga agaactgtcg caggcgatgg cggatgcgtc
    12601 gatctgcggc ctgggacagg ccgcaccgaa tccggttcgc tgtgtcatca aatacttctc
    12661 tcacgaattg aagtgataca gggacgagag atgaggggcg aggggcgagg gatgaggaat
    12721 taggaattga gggaacggcc ggcgcgcggc cgcgcctctt ctagcctcta gcctctagcc
    12781 tctagcccct agtccctcgc ccctcatccc tcgcccctag tcccttccgt taggagtcac
    12841 gaatgaatca gcgtagcgag atgatcgcgt tcaggttgga cggcgtggaa atccacgccg
    12901 cgccgggcga gaccatactc caggccgcgg agcgctacgg cgtcgacatc ccgcgtctgt
    12961 gttacaagcc cggctatcgc cccgacggca attgccgcgc ctgcatggtg gagatcaagg
    13021 gcgagcgggt gctggcgcct tcgtgctgcc gcgcgccgaa ggccggaatg gaagtcgaat
    13081 cgaagaacgc gcgggcggtg gcggcgcaga agatgattct ggagctgctg ctttccgaca
    13141 tgcccgagca gaagtattcg cgggaatcgg aactcgacct ctgggcgcgc aatctgaaag
    13201 tcggcaagcc gcgctttgcg gggcgccacc aacccaaggc ggatctgtcg catcccggga
    13261 tcgcggtcaa tctcgatgcc tgcatccagt gcacgcgctg cgtgcgtgcc tgccgagaag
    13321 agcaggtcaa cgacgtgatc ggttacgctt tccgtgggga tgaatcgcaa atcgttttcg
    13381 atctcgacga cgccatgggc gattcgactt gcgtggcttg cggtgaatgc gtccaggctt
    13441 gtccgaccgg cgcgctgatg ccggcgcacg gcgtgggcat gaaggtcgca gacaggaaag
    13501 tggattcggt gtgcccgtac tgcggcgtcg gctgccagct cacctaccac gtcaagggcg
    13561 accgcatcct tcaggtgacc ggccgcgatg gcccggccaa ccatgggcgc ctgtgcgtga
    13621 aagggcgcta cggcttcgat tacgtgcaca accggcaacg cctcaccaaa ccgcttatcc
    13681 gaaagtccgg cataccgaag agcgccgatt tcgtcgtcga tccggacaat tttctcgacg
    13741 tgttccgcga ggcgagctgg gacgaggcgc tcgatctggc cgccggccgg ctcaggggaa
    13801 tccgcgacag ccacggcaaa cgcgcgctgg caggtttcgg ctccgccaag ggcagcaatg
    13861 aagaggccta tctgttccag aaactggtgc gcaccgggtt cggttccaac aacgtcgatc
    13921 actgcacacg gttgtgccac gcctcgtcgg tcgcagcgct gctggaaggg atcggctctg
    13981 gcgcggtatc caaccaggtg tccgactgca tgaacgcgga agtggtcatc atcatcggcg
    14041 ccaatcccac gatgaaccat cccgttgcag cgacctggtt caagaatgcg gcgaagaagg
    14101 gggtcaagct catcgtcgcc gacccgcgcc gctcggacct ggcgcgtcac gcgacgcatt
    14161 tcctgcagtt caagcccgac accgacgtgg cgctgctcaa tgccatgatg cacgtcatcg
    14221 tttacgaagg gctggtgaac gaagctttca tcgcgagccg caccatcggt tacgacgatc
    14281 ttaagaagaa cgtcgagggc tatagcccgg cacagatggc gcccgtgtgc ggggtgccgg
    14341 cgcagatcat ccgggaagtg gcacggctct acgccagctc gaaggcctcg atgatcttct
    14401 ggggaatggg catctcccag cacgtacacg gcaccgacaa tgcgcgctgc ctgatagcgc
    14461 tggcgctgat gaccgggcag gtcgggcggc ccggcaccgg gctgcatccc ttgcgcgggc
    14521 agaacaacgt gcagggagct tccgacgctg gattgatccc gatgatgttt cccgattacc
    14581 accgcgtgga caatgtcgag ttccgcggac agtacgagaa gctgtggggt gtggcgctcg
    14641 atcccaggcc ggggctgacg gtggtcgaaa tcatgcacgc ggccaggaag cgcgagatcc
    14701 gcgggatgta cgtcatgggc gaaaacccgg cgatgtccga tccggacctc gatcacgcgc
    14761 gagaagcgct ggccgccctc gactggctgg tagtgcagga cattttcctg accgagacag
    14821 cgtatctcgc cgatatcgtc ctgccggctt ccgcctggcc tgaaaagacc ggcaccgtga
    14881 ccaataccga tcgcatggtg cagctcgggc ggcgcgcgct ggacatgccc ggcgacgccc
    14941 gccaggacct gtggatcatc caggaaatgg cgaggcgatt cggactggac tggcgctacc
    15001 aggggccgga aagcggcgtg gcagaagtgt tcgacgagct gcgccgcgca atgcagtcga
    15061 tcgccggcat tacctgggaa cggctggagc gcgagagttc ggtgacctac ccatgcgaac
    15121 acgaaggcga ccccggcgaa ccggtggttt tcaccgatca ctttccgacg ccgaccgggc
    15181 gcggcaagtt cgtgcctgcg gacgtcattc ctgccgcaga gcgtcccgat gcggaatacc
    15241 cgctggtgct catcaccggt cggcaactcg aacactggca caccggcgcg atgacgcgcc
    15301 gcgcttcggt gctcgatgcg atcgaaccgg atccagtcgc atcatttcat ccgctggatc
    15361 tggcaagact ggctatccgg cccggagacg tcgtgaccgt ggaatcgcgg cgcggcagga
    15421 ttgcgctcta cgcccgcgcg gacgacggca cgcccgcagg cgcggtattc atcccattct
    15481 gcttttacga agcggcggcg aatctgctca ccaatccggc gctcgacccc gtcggaaaga
    15541 tcccggaatt caagtactgc gcggtgaaag tgacacgcgg cggaaaaatg gcggcgcaga
    15601 tgagctttgg tggcggcaag ccggtggccg cggttgctgg ctagcacggc gttgtttcca
    15661 taagctgcaa tgggcaagcg gacatatgag tacatcgtgc ggatggagcg gatccgcatg
    15721 gcctacggca atgtaatcgc gctgaacgac gtgagcctta ccgtgggccg cgacgagatc
    15781 gtcggactga tcggcgataa cggcgccggc aagtccacgc tgatcaagat catgaccggc
    15841 gtagtcagac cgaccggcgg cgaactttac atccgcgatg aacggatcga tctttcgcat
    15901 tattcggtgc gccaggctca caaccggcgc atagaaacgg tctatcagga gaagagcctc
    15961 ggcgagaagc agccgctgtg gcgcaacttc ttcgtggggc ggcagatcac caaccgcttc
    16021 ggtttcatcg acattaaaaa gcagaaggca atcaccaatg acgtgctgaa gaaccagatc
    16081 gggtttcgcg gcgccggtgt cgatgccgac agcccggtgc agaagctttc cggcggcgaa
    16141 cgccagggca tcgcgatcgg gcgagccatg tacttcgatg ccgatctggt cgttctcgac
    16201 gagcccaccg ttgcgctcgc gatcaaggaa gtgcagaagg tgctcgactt cgtgcgttcc
    16261 atcaagcagc gcggacgcgc ctgcgtctac atcgagcaca atctggcgca tgtccatgaa
    16321 ctggccgaca gactcgtcat tctggatcgg ggagagataa gcaaaatttt ccacaagggc
    16381 gaagtcacgc tgaccgaact cacccgtttc ctccttgatc tgcagaacga caacgccgag
    16441 gtcgccgtct gatgcagcgc atatcgatgt cccgggttga aggcctgccg atcatcgtcg
    16501 tgtttgttgc gctgatcggc tgcttcattg ttgccgcgcc ggaagtattt ctcagcctcc
    16561 ccatctactt ttcgttcctg accacggtcc cgccggtgct ggtgctggcg ctgggcctta
    16621 ccttcgtgat cgccgcgggg gagatagacc tttcctttcc gtcggtcatt gccttctccg
    16681 gttacctgct ggcaatgctt taccgggacc acgacatgat ctggctggcg gtgttggccg
    16741 ccatcgccgg cggcgccctg atcgggctga tcaacggatt gatcgtcgct tacatcggcg
    16801 tgccgtcgat catcaccacg ctggcgaccc agttcttctg gggcggcatc accaccgtca
    16861 tttcgggcgg cacctcttat agtctgcgaa ccgtcgatga gacgtcgatg tgggtcgtgt
    16921 tcgttggcaa gatcggcgat ttcccggcgc aggcgctgtg ggccgctggc ctggcggtgt
    16981 tgttgtggtt catcatgaac cggcatcgct tcggcgagca cgtcctgttc atcggcgaca
    17041 atgccaacgt cgcgcgcgtg gtcggcgtca atgttgcccg cgaacgggtg aaactgttta
    17101 ccctgatggg aacgctcgcg ggtttcgccg cgatcctgct gacactggag aatcgcaact
    17161 tcttcaatac gcaaggccag ggttatctgc tgattgcgct cgcttcggta ttcatcggcg
    17221 gcacgtccat cttcggaggc caggcgacga tcgtcggtac gtttttcggt gctttcatca
    17281 tctcgatgat cgaggcgggg ttgacggcga cagggattca gggtttctgg gtgcgggccg
    17341 tggtcggact ggtgttcctg gccgccgtca tcttccatct gacgatggaa caaccgcagc
    17401 gcagggcaaa gcttgcgcgc gtattcggta taaaaccggg gaagtgaacc ggagtatccg
    17461 ttcagcggca cggtgccgtt ggccggaaag cgctgtatct cggcgctcgg gacgtggggc
    17521 aggggatcaa tgccccgaca acaagcgacg ttacacgaca tggaggaacc aacatgaaag
    17581 gcaaaattct ggcagcgacc gccgccgcgg tcattttggc ggcgctttac gttccctcgc
    17641 aggcggcgga gccgcttggc aagggcatga cgatgtacat gcagatgggg ggcaatgccg
    17701 gagacggcgc gacctgggcg cggactaacg gtgccctggc ggcggccgat gcgttcggga
    17761 tcaaggtgat cgagcagtat tcggcctggc agccggaaaa gatgctcgaa cattttcgcc
    17821 aggcgcttgc cgccaagccg gactgcatcg gcatcatggg ccatccgggc aacaatgctt
    17881 tcgaaccgct ggtgaagcag gcggaggacc ggggcattct cgtcaccgtc ggcaactcgc
    17941 cgctgaccaa gatccagaag atgtacgggt cgaagggcac cggctatgcc ggcgtcgaac
    18001 tgtatgccgg cggtgcgatt acggcgtcca agatgctcga ggcaggactc aagtccggcg
    18061 acaaggccct ggtctacggc ctcctgagcg aggccgagcg cggtcaatcc gacaaaggcc
    18121 tgaaggacac gctggagaag gcaggagtga aagtcgatta cctcgagatt tccgctgaag
    18181 tgaactccga cagctcgctg gcggtaccgg tgttgacggc ttacatcgca aagaacccga
    18241 cagtgaaagg gatcggtacg cagcacggcg gcgtcacctc cgtgattccg aaggcgctgc
    18301 aggcggcagg caagaagccg ggcgacgtca tcgtcggcgg gatcgatctt tctcccgcga
    18361 ccatcgacgg cctgcagaag ggctggatca ccgtcacgct cgatcagcaa ctctacctgc
    18421 agggcttcct cccggtgatg cagtgcgtgc tgtcgaagaa gtacgggttt gccggtctct
    18481 cgatcaatac cggtgccgac gtggttacgc cgaataccat caagacattg gttccgttga
    18541 tcgagaaggg catccgctga gttgttgttc cgggcgcgcc caggtgcgtc cgggcccgac
    18601 cccggcaata attcggaaca agaagccatg aaaaccatcc tcatcaccgg cgcggccggc
    18661 gacgtcggta cgcatctgcg ccgcgaactc gccggcaagt acaatctgcg gctttcggac
    18721 atcaggccgg tgaagaagct cgccgccgga gaaaaatcca tgcccggcga catcgcgcgc
    18781 atggccgaca tgcggcgcgt gaccaaggac gcggacgcga tcgtgcatct cggcggcttc
    18841 gcggtcgaag gaccgtggga catgatcctg cgcgccaaca tcgccggctg ttacaacacc
    18901 ttcgaagcgg cgcgggagaa cggcgtaaag cgcatcctgt tcgcttccag caatcacgcc
    18961 accggtttct accgccgcga ccagaagatc gatcatcgcg tatacccgaa gccggacagc
    19021 cgttacggcc tgtccaaggt attcggggaa cagctcggca gtctctacgc cgacaagtac
    19081 ggcatgcagg tattctgcat gcgcatcggc aatgtgaacc tcgcaccggc ggacaaacgc
    19141 cggctttcga tctggatcag cccgcgcgac ttcgcgcaac tggtgataat cggcgtcgag
    19201 catccggaca tccgcttcga gatcgtgtac ggggtgtcgg ggaaccagcg cagttggtac
    19261 gacaattcca atgccgagcg gctcggttac cggccacagg acgatgccga ggcctacgct
    19321 gccgcagtac tggcaaaaga gaaacccggc gccgacccgc gcaccgagat gttccagggc
    19381 ggcaccttcg tgctcgccga gatcggcggc gacccgagcc gcgcattacc gaaaatcaag
    19441 aagaaaaagc gatgagcgag gttccgaagg ttcccgcacc agccggaagc tgcgacacgc
    19501 acatgcactt ctacgaccgt gccgtgccgt ccgcacccgg cggcccgcca ctgcccggcc
    19561 actacacggt gacgatgtac cgggagctgc aaaagcagct tggcctggag cgggtcatag
    19621 tggtgcagcc caacgcctac tagtacgaca atcgcgtgac gttatccgca atcgcggaac
    19681 tgggctcgcg cgcgaaaggc gtcgcggtgg tcgggcccga cgtctcggat gccgagatcg
    19741 agcggcttac cgcggccggc gtctgcgcgc agcgcatttt tgcattgccg ggcggggcgg
    19801 tcggtttcga ccgcatggac cagatcatgg cgcgggtgca ccgctacggc tggcacgcga
    19861 acattcagtt gaatggccgc gatctgccgc gttacgaggc gcaactccgg aggctccccg
    19921 gtcatttcgt catagaccat gtcggcaaat acatcgatcc ggtgccgcct gaacacgagg
    19981 cgtttcaagc gctgcggcgg ctgctcgata ccgggcgctg ctgggtgaag ctgtccgcac
    20041 cctacgaatc gtcgaaatcc ggcgcccctg cttacgacga cgtggcgcgt ctggcccggg
    20101 aactggtgaa gcacgcaccg gaacgcatgc tgtgggccag caattggccg catccgtatc
    20161 gcagcccggt tccgtcgagc gccgcgatgc tcgatctgct ggcggactgg gcgccggacg
    20221 atgccacgcg ccggcaaatc ctggtggaca atcccgcagc actctacgga tttccacgat
    20281 gaaaaaggcg cgcatcgcca tcgtcgggct cggcatggcc gtcgtgccgc acgcaaaaag
    20341 cctgatcgag ctctcggacc gggtcgaggt cgcgtatgcc tttagcccga gcccggcacg
    20401 ccgcgcgaag ttcgcggaga cctttgcgtt tccgcagtgc caaagcatgg aggctgttct
    20461 caatgaccgc tcgatcgatg cggtcgccgt gctcacgccg gcgaacacgc acctcgacct
    20521 cgtgaggcgc tgcgccgtgg ccggaaaaca cgtgctggtc gagaagccgc tcgagattac
    20581 caccgccaga gctgaggaaa tggttgccga ttgccgcgcc gccggcgtca cgctcggtgt
    20641 cgtcctgcag catcgccaca agcccgcagt gaagcgcctt gcgagtgcgc tggcctcggg
    20701 cgaactgggc atgctggtcg gggcatcggc cgtcgtccgg ctgtggcgtc cgcagagtta
    20761 ttacgacgaa cccggaaggg gaacccgtgc gcgtgatggc ggtggggtcc tcattactca
    20821 ggcaatccat acgctcgacg tgttgctgtc gcttgccggc gacccgcagg aggtgatgag
    20881 ctatgcggcc accacgccgg tccatcgcat ggagtgcgag gatctggtgt gcgcggccgt
    20941 ccactggggc agcggtgccc tgggcgtcat cgaggccacg accgctgctc atccgggtgc
    21001 ggcggagcgc atcgaactga ccggcacgcg cggcacggcg agtctcgccg gcacggcgct
    21061 ggagctgcgc tggcacgacg gcggcagcga gaccatggcg gcggacggtt cggcgggcgg
    21121 cgcaggcgcc gatccaatgg cgttcccgca cgattatcac cgtagtgtgt ggcgggattt
    21181 tattgatgcc gtccagagcg gcggcgaacc gcgcgtcaac ggcgcggagg cgctgcgcgt
    21241 gcaccggttg atcgacgcgc tgctggcgag cgccgctacg ggtaaggcgg tccgccgcaa
    21301 tttggctgca gggggaggca catcatgaat tcggattcac gccgtcgttt tctggcgtcc
    21361 gcttccgtag cgacactcgc gctcgccggc gggatgcggc gggcccgcgc ccaatccgcc
    21421 gtgacgctgc ggatttcctc gtcgctgccg gccgaccaga acgcggcgca ttacccctgg
    21481 taccaacgtt tcgccgccaa tctgcaaggc gcgactggca acggcattgc gatcgagtat
    21541 tttccgaaca gccagcttgg caaggaagcc gacgtggtgc agcaggtgaa agtcggatcg
    21601 atcgacatga tgatcacggg atcgtcgatc tgggccaccg tcgcgcccga attcggcatg
    21661 ctcgacctgg gatacgtgtt cgacagctat gcgcacgtgg cgaaggcgct ggacggcggg
    21721 gtcggcgagc gtctggccag gatgcttttt gaccggacgg gctgcacggt gctgggctgg
    21781 gcatcgcact tcggcgcgcg cagcgtctac gccaagaacc cgatcaagtc gcttgccggc
    21841 gtcaagggcg tgaagctgcg cgttctgcct acgacggctt tcatcgagac cttcaagctc
    21901 atgggagcaa tcccgacacc gattcccttc aacgagttgt acaccgcgct gcagaccgga
    21961 gtggtggacg gcttcgagca tgacgcggcc acggtagtcg ccagcaagct ttatgaggta
    22021 tcgaaatact gctggctgac ggaacatttg ttcagtccga tgatcgccgt catgggccgg
    22081 cgcggtctgg agaaaatccg cgccgacgtt cgcccggtgc ttttcaaggc ggcggcggaa
    22141 gcgaccgcct atcagcgcgc gcatgccgcg gagaagggtc gtgcggcggt cgaggagctg
    22201 aaacgcctgg gcatcacgtt ccagccgatg gcaaaaaccg agcgtgagtt cgtccgcaag
    22261 gaaatggaaa cgaagctgtg ggcggcgttt gcggcgcaat acccggcaac caagccgctg
    22321 ttcgaagcca tcgcggcggc ccgggcatga cccggcccgc cttgcgggca ccatcgccgc
    22381 caccggtcgc gcggtctgac cggttggcat gaacccgcac gacgccaggc cctacgccaa
    22441 ggggttcgca agcgcaggtg gggcacatcg cctgttggcc gggttgctgg ctgtcatcga
    22501 gtacttcgcc gcattcgtcc tcgcggtgga tgtgctgatg gttttcgcct cggtaatctg
    22561 gcgttatttc ctgcatgacc cgctgaactg ggccgaggag atcgcgcgcg cgctgatggt
    22621 cacgcaggtg tttctgggtg ccgcgaccgc agtaggcagg gcccagcatg tcggcatcga
    22681 cagtttccgc accctgtttc cgctgagctg gcgcccggcg atgattcaac tgtgcgactg
    22741 gatcatcgtc gcggtttctg ttgctctgct gttctcgtct gccgccatgc tgatcgattc
    22801 gcgcgaccag accacgccga tcggattgcc ccaatggatt tacatttggc cggtggtagc
    22861 cggaagcctg ctgatgatcg tgttcggaac cgccgcggcc ctcgatgggc gctggaagca
    22921 cgtgtggggg acgttggctg ccaccctcgg gctggcactg gcggtatggg gctggaacac
    22981 gacggtcccg acgcacgcgg tcacgccggc ttttttgctg gtcgcgggat tcgtcgcaag
    23041 cctggtgatc ggcgtgccga tcgcatttgc gctggcattt tcgtcgctgc tgtattttct
    23101 ggtcgatccg tccttgccga tgctggtgta ttcgcagcaa gtcttgtccg gaatggatca
    23161 cttcgtgctg ctggccatcc cgttctttgt gctggccggt ctgatcatgg aagccaacgg
    23221 catgtcggcg cgactgatcg aattgctgct gaggatgatg gggcggctac ggggcgggct
    23281 gaacctgatc atgattgccg ccaccgcgtt tttttccggc gtgtcgggat cgaagctggc
    23341 ggacatcgcc gcagtgggcg gcatcatcat gccggcggtg cgcaggacca gacaggatcc
    23401 caacgacgcg gccgggctgc tggccagcac ggcggtaatg gccgagacca ttcctccgtg
    23461 catcaacatg atcatcctgg ggttcgtcgc caatatttcg atcggcggac tgttccttgc
    23521 cggcttggtg ccggcgggcg tcctggcgat ggctctggcg gcggtcgcgg tctactgcgg
    23581 atcccgcatc aacccgaatg acgctttcga ggtgcagacg ccgctgatac gcctgatcgg
    23641 cggagcgatg gtcggcctgg tcatggtgct catgatcggc aagggcgtgg tctccggcgt
    23701 agcgacctct accgaggtct ccgccttcgc cgtcatctac gcactggttg tcggcggcct
    23761 ggcgttccgc gaactgagcg tgaagtccat cgtcgggctg ttcgtccgtt ctgcatccat
    23821 ggccggcggg attctcttca tcgtcgccgc cgcatccagc gtttccttcg cgttgacgat
    23881 ccagcagatt ccacagtatc tgtcggatac catggtggcg ctcggccatc agcacggtcc
    23941 gcaggccttc gtggtggtct cggcgctgct gatgatcgtg ttcggggcga tactggaagg
    24001 ggcgcccgcg ttgatcatct tcggtccgct gttggcgccg atcgcccagc agctcggcgt
    24061 gcatccgctg cacttcggca cggtgatggt catcgccatg gggctcggcc tgttttcgcc
    24121 gccgatcggc ctgggactgt tcgccacctg tgcgatcacc ggcactcaaa tgaaggacgt
    24181 tgccgggccg atgctcaaat acctggccgt tctgttcgcc gggttaatgc tgctggtttt
    24241 cgtgccggca ttttccttat ggctgccttt tcacatgggc atgaaatagc gtaatggggc
    24301 cctcatggct ccgatgagct ggcatggctg gctgatcttc gccatggtgg cgctgccgct
    24361 gattggtttc ctgctgtgga tattcggcca gttcctgagg gccaggcgcg aggaaaccga
    24421 aacggttctg cgcaaaggcc tgccggcgca agcggaaata atcgcctgcg cgcctggttt
    24481 gcggggcgca acggttcgat accggtttct cgccccgggc tgggagcatc cgatcgtcgt
    24541 tacgcagagg ctcccgccag gcagcaagtt cgcggtcggc gataaagtcg cgatacgcta
    24601 cctgcccagg catccgcata ttgcggtcat cgttcccaag gcgaaatgaa ccgaagcaag
    24661 aaaaaagctc agctcttgcc acggaggaca cggaggaaaa ccaaaacgag gaaaagtaaa
    24721 ttggagtcaa agcgtgatga ctgttcgcga acctgcgccg gcaacttttt actgcagtgg
    24781 atcatcagcc caaaggtctt cctcgaccca tttttctctc ttgcctttcc tccgtgtcct
    24841 ccgcgtcctc agcggtgagc gttgtacttt cctgaccctt cagccgagct aacgcacggt
    24901 gccgcgcgcc gcgactggcc aggtgacctc gacccgctct ccgcgcacgc cgtagacctc
    24961 gtccaccatg ttgctcacca cgcagcagtg gttgggcact acgtggacga tgtcgccgac
    25021 cgcaggccgc tcccggcagc ccgaaag
//
LOCUS       DASQOP010000100        23138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2255480, whole genome shotgun sequence.
ACCESSION   DASQOP010000100 DASQOP010000000
VERSION     DASQOP010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 23138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23138
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2255480"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="VE030_03980"
     CDS             complement(<1..1064)
                     /locus_tag="VE030_03980"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011694362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HZF22609.1"
                     /translation="MADNWRSNLITAGVARSPNRAMLRAVDFNDGDFEKPIVGVANGH
                     SNMNPCNAGIQPLVDRAMRALREAGAMPQVFGTPTVTDGIGMGTDGMKYSLVSREVIA
                     DAIETAVNGQMMDGALVIGACDKNMPGGMMAMARMNVPAIYVYGGTIKPGNWKGQALT
                     IASAFEAVGAFTAGKLSQEDFDGIERNACPTVGACGGMFTANTMSSSFEALGLSLLGS
                     SQLAAIDPQKADSAADSARVLVNAIKKNIRPRDIITRKSIENVVSLVMATGGSTNAVL
                     HYLAIASAAEVEWTIDDFERIRRKVPVLCDLKPSGKYVAVDFHKAGGVPQVLKMLLVN
                     GLLHGDCMTVTGRTIGEELE"
     gene            1218..2138
                     /locus_tag="VE030_03985"
     CDS             1218..2138
                     /locus_tag="VE030_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZF22610.1"
                     /translation="MELRHLRYFVMVAEELHFGRAAEKLHISQPPLSMQIRSLEEELG
                     VTLLHRTQRHVSLTQAGHAFLQEARQILARLEQAVLMTRRAGRGEIGELAVGFISVAD
                     YNVLPVVLREFRQRFPLVNLTLRESTTDAQIRDLLGGRIDVGFVLPPIDEPALQSVCI
                     VREPLVAALPDRHPLARRPGKLALANLKDAPFILFPRPMAPGLYDDVVSFCRAAGFSP
                     RVEQEAIQMQTIVSLVSAELGVALIPASLTNLRRTGVTYKALREASPLTEIHLAWRRG
                     DELPALRVFVDLALQAAGSRAAAKSKRSTA"
     gene            2135..2608
                     /gene="tsaE"
                     /locus_tag="VE030_03990"
     CDS             2135..2608
                     /gene="tsaE"
                     /locus_tag="VE030_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HZF22611.1"
                     /translation="MNSAAVELSAFLPDEAATLGFGEMLAQILRPGLFVALSGELGSG
                     KTTLARGILRGLGYEGKVKSPTYTLVELYKLSKLNLYHFDLYRFNNSHELLEAGFRDD
                     FDSRNVCMIEWPERAQDLLPTADLSVSLVEERGGRRVRIAAQTELGKNCLARIAS"
     gene            2584..3930
                     /locus_tag="VE030_03995"
     CDS             2584..3930
                     /locus_tag="VE030_03995"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HZF22612.1"
                     /translation="MSGTDRILKQWNAGPGKNCGRQRSCRRVVLLATALAAFLAALSG
                     AHGATQVSSARIWPAQEYTRITLESGAPIAHNVFTIANPYRLVLDLENVESSTALDVL
                     STKISPDDPYVSGVRIGRFKPGVIRLVLDLKAQVKPQVFTLTPVGDYGHRLVLDVYPV
                     EPVDPILALLEKNIVPAPAAGSAKPATQPDQPASGATRAPAVPGAKKPEVLRLVTVAV
                     DAGHGGEDPGAKGRSGTYEKHVTLAIAKKLKALIDDEANMRGVLIRDGDYYIPLANRV
                     VKARKVQADLFVSIHADAYIKPHARGSSVFALSERGATSAAAGWLAKKENEADLIGGV
                     NIDVDDPTLKQVLLDLSQTATINYSLKLARAVLGEIGGINNLHRAHVEQAGFAVLKAP
                     DIPSILVETAFLTNPDEESRLKDEKYQDRMAQAILVGIRKYFAANPPLARSKLAQN"
     gene            4063..4626
                     /locus_tag="VE030_04000"
     CDS             4063..4626
                     /locus_tag="VE030_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006480889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HZF22613.1"
                     /translation="MTVTGILLAAGSGSRFGGAKLLHRLRDGTPIGVASLRNLRKALP
                     DVIAVVRAGDDELSNLLESEGIVVKDCEDAYLGMAHSLVCAIRASMSASAWVIALGDM
                     PYVAPATISTVARHVAQPGGIVVPAYRGKRGHPVGFGSSFRAELLGLEGDEGARSVVE
                     RHAQTVETIDCDDPGILRDIDTPGDAL"
     gene            complement(4633..7458)
                     /locus_tag="VE030_04005"
     CDS             complement(4633..7458)
                     /locus_tag="VE030_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22614.1"
                     /translation="MDKKTIYSKTGKGVLEIKNKAGKLARDLVKVLTLIDGKSTVAEL
                     IAKSKLSDADIDRSLLQLTTGGYIKEFSNTSGSASSTPLESSYVDDLDFTSSLAPGKN
                     PYQSAQTEFRQRESADRVKAEEEAKKKREEEERLKKEQALRQARDEAARLAKVEAERK
                     AKEAAALKLREEAERKAKLEAEALAQTQRDLGKILEAERKALEQSDRTKQEAFERDAQ
                     ENAERRNREDAQRQRREEEEHKTREVEEHRGREEEERKRKEEEEGRRKVDEERRRREE
                     EERKRRDEEERKRKEEEERKRRAEEERKLKEEEQRKRREEEERKKREEEEQRGRREEE
                     ERRLRREEEDRRRAEEDQRRRREEEERKRREDEERARSEAAAKNDLPEFDLSGLRSME
                     SQIASEFEKQQEELRRREQEEETRNREQEESRHAIERAEREEEARLEAERREAEDRER
                     RGRMERERQDREERERKRNEEKELRIKEQEERKVKAGLERERQEQAKIESDRRAREEE
                     LARRRKEQDERDRKRAEVQSLKKHKGIRTPLERFRPAIIAVVVVVALVVGGIQLIPMN
                     TYIPSIERLASGHIGEPVTIGSVRVSVLGGFSMSLDNVKLGTTQDVKIDKVDLSLDLG
                     SVFDEVKIIRNVTVESASVAQEVLPRLPKWMEAAVTDKNLQVKRVVFKGVKLESRTVK
                     VPNFDAELDLSPEGAVRKAALETTDGKLSVQITPRGEELDIAVSAGKGWVPPIGPQIE
                     FTDFSAKAVASGHQIRVAEFRALLYGGAANGSALINWGGPWSIEGQVNSERVALQELM
                     SVFTRDAKSTGHLEATLRYSLAAPDLVTLFDTPRIEGTFVTRKGDLDGVDLVRALQMG
                     GRQNVQGGATRFEEISGALTVAGGRYQYRNLKLASGLLSATAGLEVLPNKEVHGRVFV
                     ELRSQAAQIRGNFIVDGNLKAIVLKPN"
     gene            complement(7747..9129)
                     /locus_tag="VE030_04010"
     CDS             complement(7747..9129)
                     /locus_tag="VE030_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HZF22615.1"
                     /translation="MSANLVALAYLVASVFFVLSLKGLSSPLMARRGNLFGIIGMVIA
                     VLTTLSVTQNWMLIVLGVAVGGSVGAVVARRIQMTAMPELVAAMHSLVGLAAVLIAFA
                     AVHNPGAYGILTADGVIPRGNRLELFIGTFVGAITFSGSVIAFGKLSGRIRSAPVVFG
                     GQHWVNLVLAIVMIAFGVAFMFNQSWTPFAIMTTIAFVLGVLIIIPIGGADMPVVISM
                     LNSYSGWAAAGIGFSLNNPMLIISGSLVGSSGAILSYIMCKAMNRSFFNVILGGFGGG
                     DTAAATGDVAQKTHRSGSPEDAAFLLGNAESVIIVPGYGLAVARAQHAVKEMAEKLHE
                     KGVKVRFAIHPVAGRMPGHMNVLLAEAEIPYDQVFEMEEINNEFPTTDVVLVLGANDV
                     VNPAANVPGSPIYGMPILEVHRARTVLVVKRSMAAGYAGLDNDLFYMDKTMMVFGDAK
                     KVVEDIVKAI"
     gene            complement(9221..9520)
                     /locus_tag="VE030_04015"
     CDS             complement(9221..9520)
                     /locus_tag="VE030_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-translocating transhydrogenase family
                     protein"
                     /protein_id="HZF22616.1"
                     /translation="MEAIDPIVNHLTVFVLAIFVGYHVVWTVTPALHTPLMSVTNAIS
                     GIIIVGAMLAAGPQPIDWGTVLGFVAVVLASVNVFGGFLVTQRMLEMFKKKDKRT"
     gene            complement(9606..10730)
                     /locus_tag="VE030_04020"
     CDS             complement(9606..10730)
                     /locus_tag="VE030_04020"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HZF22617.1"
                     /translation="MQIGIPAEIRAGETRVAVTPETVKKLAGSGHHKLLVQSGAGAGC
                     SIPDSEFAAAGAAVAHSAAEAYGCDIVLKVRGPEPGELAMLKSGGVLIALLAPYKKEG
                     IEAIARTGVTAFAMESLPRISRAQSMDVLSSQANVGGYKAVMLAANAYQRLMPMMMTA
                     AGTIKAARVVIMGVGVAGLQAIATAKRLGAVVEATDVRPETKEQVESLGAKFVEVPLT
                     EAEKELAKGQGGYAREMGDDYKKRQAALIAERIKQADIVITTALIPGRPAPLLVTEDM
                     VKTMKPGSVIVDMAVEQGGNCPLSELDKTVVKHGVQIIGIANLPALVPADASALYARN
                     LLNFLNLLLDPKTGQLQLNREDEIIAGSLVCIGGEPVKKQ"
     gene            10990..12753
                     /gene="mutL"
                     /locus_tag="VE030_04025"
     CDS             10990..12753
                     /gene="mutL"
                     /locus_tag="VE030_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HZF22618.1"
                     /translation="MPAIHLLPDLLISQIAAGEVVDRPASALKELLENSLDSGATDVT
                     VQLAEGGIRQIRVADNGAGIGKDDLALALARHATSKIASLEELERVASLGFRGEALAS
                     IAAVSHLSLLSRTSDSPHAWRLDASGGALSAAEPAAAVAGTTVEVRDLYFNTPARRKF
                     LRSPATEFAHSEESFWRIALSRPDVRFTLTHNGRAQSHLHPAGSEERIHAVLGEAFRE
                     AAIPVQAEAGSFRLRGLVAQPTYSRGARDAQYFFVNGRYVRDKLLAHAVRQAYHDVLH
                     HERHPAYVLFIDLDPALVDANVHPTKIEVRFRDSRGIHQFVFHALEKALAGTRAGNPP
                     AQEVGAAMAFPRAGTSAFRQSGMDLRAAQPAAFYATLFGAGQATLEAPARAPAALPQD
                     APHPLGFALAQLAGIYILAQNDEGLVVVDMHAAHERIVYEQLKSALDVDRIPTQQLLI
                     PATLTASALEVATVEDHAQTLSRLGFDLAAIAPNAIAVRAVPMTLAQADSAELARDVL
                     REIAQFGGSRVLAERQNEMLSTMACHAAVRANRSLTVAEMNALLRDMEATERSGQCNH
                     GRPTWFQVTIGELDRMFMRGK"
     gene            12941..13849
                     /gene="miaA"
                     /locus_tag="VE030_04030"
     CDS             12941..13849
                     /gene="miaA"
                     /locus_tag="VE030_04030"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HZF22619.1"
                     /translation="MGPTASGKTGVALELARHFPVEIVSVDSALVYRRMDIGTAKPDL
                     ATRLEVPHHLIDIIDPTGNYSAAQFCDDALEAMRGIVERNRVPLLTGGTMLYFKALQE
                     GLSDLPAADADIRMVIDAMAVKAGWPAIHRELERIDPATAERLDPNDAQRIQRAMEIF
                     YLTGKPMSELIAAGRSASLPYRMIPIALIPGDRAALHRRIAARFEEMLELGLINEVRA
                     LREDYDLKPSLPSMRCVGYRQVWQYLDGEFGLAALRDKAVAATRQLAKRQLTWLRAIP
                     GVKAFDGLDEGLKGHIEAWLPEQLGR"
     gene            complement(13889..14311)
                     /locus_tag="VE030_04035"
     CDS             complement(13889..14311)
                     /locus_tag="VE030_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HZF22620.1"
                     /translation="MNANAQLADKKVLTLEGAKKIAAAAEAEARKNNWNVVIAVVDDG
                     GHLVYLQRIDGTQTGSIEVAIQKARTAQAFKRPTKVFEDAIAGGRNALIALHGALPLE
                     GGLPIMVGGQLVGAIGVSGVKSTEDGQIAKAGADSLAN"
     gene            complement(14502..15851)
                     /gene="xseA"
                     /locus_tag="VE030_04040"
     CDS             complement(14502..15851)
                     /gene="xseA"
                     /locus_tag="VE030_04040"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HZF22621.1"
                     /translation="MRYMISQDPANIVISVSELNRRARTLVESGFPLLWVAGEISNFT
                     RATSGHCYFLLKDAQAQARCVFFRHKASALDWQPENGMQVEVRAAPTLYEARGDFQLN
                     VETMRRSGLGALFEAFERLKKKLEQEGLFETSRKKPIPAFPLVIGIVTSPKAAALRDV
                     LTTLARRMPAIRIVLYPAPVQGEGAGERIAQAIRSASARADADVLIICRGGGSIEDLW
                     AFNEEVVARAIADCAIPVIAGIGHETDFTIADFVADARAPTPTAAAELASPNREELLV
                     RLGALYRQLKHSADRLLEARMQQLDYLGKRLVHPGQRIRDQARHLAHLATRLKLSMMK
                     VLDTASWRVADLLGRLRTTRPDLVAAALQHRELARRLKLGYARLLELHEVRLRRLGAN
                     LEHLNPRSVLERGYSIVAKVDGRIVRSAAEVQTSERLRLAFARGTALARVESKEDVD"
     gene            16301..16912
                     /locus_tag="VE030_04045"
     CDS             16301..16912
                     /locus_tag="VE030_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HZF22622.1"
                     /translation="MLAIIEAAGWPIWPIILCSVVAVGIIGERFWSLRQDIVAPRGLL
                     AQVVQEYRQKGVSPEMLDRLALSSLMGRILAAALRNVGSTREIMRESIEEAGRAAAHD
                     LERFLTTLGTIATMSPLLGLLGTIIGMIEIFGVQGPTGMANPAQLAHGISIALYNAAF
                     GIIVAVPSLVFYRHFRARVDSLVVEMEIQAVKLVEIIQGERKA"
     gene            16933..17349
                     /locus_tag="VE030_04050"
     CDS             16933..17349
                     /locus_tag="VE030_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZF22623.1"
                     /translation="MNFRRGSFQEEPEINLVPFIDVLIVIVIFLAVTTTYSKYSELQI
                     NLPTADANRPAERPNQVNVGVSASGQYMVNRTPVTFTTREAFANELRRAASGNPDPVI
                     IINADANATHQSVIHVMEAAQMAGFGKITFTAQLPR"
     gene            17407..18402
                     /gene="lpxK"
                     /locus_tag="VE030_04055"
     CDS             17407..18402
                     /gene="lpxK"
                     /locus_tag="VE030_04055"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HZF22624.1"
                     /translation="MPFSDRHWYRLSWLSVALLPLAALFRLSASLRRLAYRIGVLRSE
                     RLPVPVIVVGNITAGGTGKTPLTLWLCGILRQQGFKPGIVSRGYGGLARLQAVTADSD
                     PAAAGDEPVLLARRSRCPVWIGRDRVAAARALLAANPECDVIVCDDGLQHYRLRRDIE
                     VVVLDGSRGLGNGLPLPAGPLREGAARLRSVDAVVINGPGGFGVRVGSRFDMSLEGSY
                     FHNLLNPGHTAAASEFHGRRLHAVAGIGNPLRFFEHLQRLGLSFTAHVFPDHHPFAPA
                     DLDFEDADEIIMTEKDAIKCQRFARENQWALPVDAAVDPALGQHILDRLKSRHGS"
     gene            18392..18571
                     /locus_tag="VE030_04060"
     CDS             18392..18571
                     /locus_tag="VE030_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HZF22625.1"
                     /translation="MDPKLLDILVCPLCKGPLVYRKEEQELICKACRLAYPIRDDIPV
                     MLEDEARKLLPTEEV"
     gene            18653..19420
                     /gene="kdsB"
                     /locus_tag="VE030_04065"
     CDS             18653..19420
                     /gene="kdsB"
                     /locus_tag="VE030_04065"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HZF22626.1"
                     /translation="MNFVVVIPARYASSRLPGKALAEIAGKPMVVHVADRARESGAAE
                     VIVATDDGRILDAVGRHGHAAMMTRAEHASGTDRIAEVAVQRGWSGQQIIVNVQGDEP
                     RIAPALIRAVAQQLAGHADAAIATVCHAIHDAAEMFDPNAVKVVIDKDGYALYFSRAP
                     IPYARDAFGPDRSRWPAGLPAYRHLGLYAYRCGFLRTYSTLAPAAIEQFESLEQLRAL
                     WHGHRISVLITDQAPEPGVDTPADLEKMRKAFAGAAR"
     gene            19513..20166
                     /gene="adk"
                     /locus_tag="VE030_04070"
     CDS             19513..20166
                     /gene="adk"
                     /locus_tag="VE030_04070"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HZF22627.1"
                     /translation="MRLILLGAPGAGKGTQAAFISEKFGIPQISTGDMLRAAVKAGTP
                     LGRKAKKFMDAGALVPDDLMIGLVKERIKQPDSAKGFLFDGFPRTIPQADAMKNAGVN
                     LDYVVEMDVDDAEIIKRLSGRRVHPASGRTYHVAFNPPRAPGKDDATGEDLIQRDDDK
                     EETVKKRLEVYHSQTKPLVAYYSKWAASGKPGAPKYVKIAGVGSMDAIRDRIFAALK"
     gene            20380..21639
                     /locus_tag="VE030_04075"
     CDS             20380..21639
                     /locus_tag="VE030_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HZF22628.1"
                     /translation="MAKRNAFYAQSGGVTAVINASACGVIETARNHKNRIGKVYAGRN
                     GIIGALTEDLIDTGRESASAIAALRHTPAGAFGSCRYKLKGLDENRAQYQRLIDVFKA
                     HDIGYFFYNGGGDSADTCLKVSQLSETLGYPIQAIHVPKTVDNDLPITDCCPGFGSVA
                     KYVAVSVREASFDVASMAKTSTKVFVLEVMGRHAGWIAAAGALAADAKTDLPIIILFP
                     EVAFDKAKFLARVDSTVKKYGYCSVVVSEGVKGADGKFLSDQGLRDAFGHAQLGGVAP
                     VVADMVREGLNLKFHWGVADYLQRAARHIASGTDVAQAYAVGKAAVELALKGRNAVMP
                     TIVRKSQKPYKWVIGVAELKEVANKEKFMPRDFISEDGFQITQKCRDYLAPLIRGEDF
                     PPFKDGLPGYVILKNVAVPKKLPAFKL"
     gene            21822..>23138
                     /locus_tag="VE030_04080"
     CDS             21822..>23138
                     /locus_tag="VE030_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HZF22629.1"
                     /translation="MSSALIFALVCAIIGIIYGAISIKWILGRPAGNARMQEIARAIQ
                     QGAQAYLNRQYTTIAIVGAVLFFVLGFGLDWYTAIGFIVGAVLSGATGYIGMFISVRA
                     NVRTAEAANHGINAALNVAFRGGAITGMLVVGLGLLGVAGYYAAILPTAGAAKALHAL
                     VGLAFGGSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADNVGDNV
                     GDCAGMAADLFETYAVTIVATMLLGGLMVESVGANGILYPLVLGGASIIASIVGTFFV
                     KTSEGGKIMNALYKGVIVSGVLAAIAFYFITTKMMADSPIAAVNLFYASLVGLVLTAA
                     LVVITEYYTATEYAPVRHVAAASQTGHATNIIAGLGVSMKATALPVLAVCASIWASYS
                     LAGLYGIAIAATAMLSMTGMVVALDAYGPITDNAGGIAEMAGLPSKI"
BASE COUNT         4143 a         7537 c         7031 g         4427 t
ORIGIN      
        1 ttctccagtt cctcgccgat ggtgcgaccg gtgacggtca tgcagtcgcc gtgcagcagc
       61 ccgttgacca gcagcatctt cagcacttgc ggcacgcctc cggctttgtg gaagtccacc
      121 gcgacatatt ttcccgaagg cttgaggtcg cacaacaccg gcactttgcg gcgaatgcgc
      181 tcgaaatcat cgatggtcca ttcgacctcc gcggctgacg caatcgccag gtaatgcagc
      241 actgcattgg tcgaaccgcc ggtggccatc accaggctga cgacgttctc gatggacttg
      301 cgggtgatga tgtcgcgcgg gcggatgttc tttttgatcg cgttcaccag cacgcgcgcc
      361 gagtcggcgg cggagtcggc tttttgcggg tcgattgccg ctagttgcga ggagcccagc
      421 aggctcagcc cgagggcctc gaacgaggac gacatcgtat tggcggtaaa catgccgccg
      481 cacgcgccca cggtcggaca ggcgttcctc tcgatgccgt cgaagtcctc ctggctcagc
      541 ttgccggcgg taaacgcgcc caccgcctcg aaggcgctgg cgatggtcag cgcctgaccc
      601 ttccagttgc ccggcttgat ggtgccgccg tagacataaa tcgccggcac gttcatacgc
      661 gccatcgcca tcattccgcc gggcatgttc ttgtcgcagg cgccgatcac cagggctcca
      721 tccatcatct gcccattgac cgcggtttca atcgcgtccg cgatcacctc gcgcgatacc
      781 agcgagtatt tcatgccgtc ggtgcccatg ccgatgccgt ccgtgactgt gggcgtgccg
      841 aaaacctgcg gcatggctcc tgcctctcgc agtgcccgca tggcgcgatc caccaaaggc
      901 tggataccgg cattgcaggg gttcatgttg gagtgcccgt tggccacgcc gacgatcggt
      961 ttttcgaaat caccgtcatt gaaatcgacg gcgcgaagca tcgcgcggtt gggcgagcgg
     1021 gcaacgcccg cggtgatcaa attgctgcgc cagttatctg ccatgttctc ccccggtact
     1081 gtggaatgcg gcttgcggat tgccgtaccc gggccgcaga cgcggcccgg gcacggatga
     1141 aggccgcaag tttggacgcg ccgcccacag gtgtctaata tattatttga ggacttttga
     1201 tactaatcgc gaatcaaatg gaactgcgcc acctgcgcta tttcgtcatg gtcgccgagg
     1261 aactgcactt cggccgggcc gccgagaaac tgcatatctc gcagccgccg ctgtcgatgc
     1321 agattcgttc gctcgaggag gaactgggcg taacgctgct gcatcgcacc caaaggcatg
     1381 tgtcgctcac ccaggccggg catgcatttc tgcaggaagc acggcagatc ctggcgcggc
     1441 tcgagcaggc ggtgttgatg acgcggcgcg ccgggcgcgg tgaaatcggc gaactcgcgg
     1501 tcggtttcat cagcgtagcg gattacaacg tgctgccggt ggtactgcgc gaattccgcc
     1561 agcgattccc gctggtgaac ctgaccctgc gcgaatccac caccgacgcc cagatcaggg
     1621 atctgctcgg cggccgcatc gacgtgggct ttgtgctgcc gccgatcgat gaaccggcgc
     1681 tgcagtcggt ctgcatagtg cgcgagcctc tggtggcagc gctgccggat cgccatccgc
     1741 tggcgcgacg tcccggcaag ctggcgctgg ccaatctgaa ggacgcacct ttcatcctgt
     1801 tcccccggcc gatggcgccg ggactgtacg acgacgtggt gagcttctgc cgagccgccg
     1861 gattcagccc gcgcgtggaa caggaagcga tccagatgca gacgatcgtc agcctcgtct
     1921 cggccgaact tggcgtggcg ctgatcccgg cgtcgctgac caacttgcgt cgcaccggcg
     1981 tcacctacaa ggcgctcagg gaggcaagcc cactcaccga aatccatctc gcctggcggc
     2041 gtggtgacga acttccggcg ctgcgcgtat tcgttgacct ggcgctgcag gccgccggtt
     2101 ccagggcagc ggcaaaatcg aagaggtcaa cagcgtgaac agcgctgccg ttgagctttc
     2161 agcgttcctg cccgacgagg cggccacgct tggtttcgga gaaatgctgg cgcagatact
     2221 gcggcccgga ttgttcgttg cgctctcggg cgagctggga agcggcaaaa ccacgctggc
     2281 ccgcgggatc ctgcgtggtc tcggatacga ggggaaagtg aaaagtccaa cctatacact
     2341 tgttgaactt tataaacttt ctaagttaaa cttatatcac tttgatttat atcgattcaa
     2401 caattcgcat gagttgctgg aggcaggctt ccgggacgac ttcgattccc gcaacgtctg
     2461 catgatcgaa tggcccgaac gggcgcagga tctgctgccc accgcagacc tgagcgtttc
     2521 gcttgtcgaa gagcgcggcg gacggcgtgt ccggatcgcc gcacaaacgg agctgggaaa
     2581 aaattgtctg gcacggatcg catcctgaag cagtggaacg ctggcccggg taagaactgc
     2641 gggcggcagc gatcctgccg gcgcgtcgtc ttgctcgcca cggcactggc cgcttttctg
     2701 gcggcactgt ccggcgctca tggcgccacg caggtaagtt ccgcgcgcat atggccggcg
     2761 caggagtaca cgcgcatcac gctggaatcg ggagcgccga tcgcgcacaa cgtcttcacc
     2821 atcgccaatc cctaccgcct ggtgctcgac ctcgagaacg tggaatccag cacggctctc
     2881 gatgtgctgt ccaccaagat ttcgccggac gatccctatg taagcggcgt gcgtatcggc
     2941 cgcttcaagc ccggcgtcat ccgcctggtg ctggacctga aggcacaggt caagccgcag
     3001 gtcttcacac tcacgccggt cggggactat ggccatcggc tggtgctgga cgtctatccg
     3061 gtcgaaccgg tggacccgat tctcgccctg ctcgagaaaa acatcgtccc cgcgccggcc
     3121 gccggaagcg caaagccggc aacacagccg gaccagcccg caagcggtgc aacccgggcg
     3181 ccggccgtgc cgggcgccaa aaagccggag gtattgcgcc tggtcaccgt cgcggttgac
     3241 gccggacacg gcggcgagga tccgggcgca aagggccgca gcggcaccta cgagaagcac
     3301 gtcacgctgg caatcgcgaa gaaactgaaa gcgctcatcg acgacgaggc gaacatgcgc
     3361 ggcgtgttga tccgtgacgg cgactactac attccgctcg ccaatcgcgt cgtcaaggcg
     3421 cgcaaggtcc aggcggacct gttcgtctcg atccatgccg atgcctacat caaaccccac
     3481 gcccgcggct cttcggtgtt cgcgctgtcg gaacgcggcg cgacctcggc cgccgccggc
     3541 tggctggcaa agaaggaaaa cgaggccgac ctgatcggcg gtgtcaatat cgacgtcgac
     3601 gacccgaccc tgaagcaggt actgctcgat ctttcgcaga ccgccaccat caactacagc
     3661 ctgaaactcg cccgggcagt gctgggcgag atcggcggca tcaacaactt gcacagagcg
     3721 cacgtcgagc aggccggctt cgcggtgctc aaagccccgg acatcccctc catcctggtc
     3781 gagaccgcct tcctcaccaa ccccgacgag gaaagtagac tcaaggacga aaagtatcag
     3841 gacaggatgg cgcaggcgat tctggtgggc atccgcaaat acttcgctgc gaatccgccg
     3901 ctggctcgtt ccaagctggc gcagaactga gagcaaggat gaaggatgaa ggcggaaggg
     3961 tgagggggaa aaacgaaagg caggaagacg gcgtgcgctc cgcgcactca taacttcatc
     4021 cttcatcctt ccgccttcat ccttgccctt tccccttcat ccttgacggt caccggaata
     4081 ctcctcgccg cgggcagcgg cagccgcttc ggcggcgcca agctgctcca tcggctccgt
     4141 gacggcacgc cgatcggcgt ggcttcgctg cgaaatctca ggaaggcgct gcccgacgtc
     4201 atcgccgtgg tgcgcgccgg ggatgatgaa ctgtcaaatt tgctggagag cgagggcatt
     4261 gtcgtgaaag actgcgagga cgcatacctg ggtatggcgc acagcctggt ctgcgcgatc
     4321 cgcgccagca tgagcgctag tgcctgggtg attgcgctgg gagacatgcc ctacgtcgca
     4381 ccggcaacca tcagcacggt cgcccgacac gtcgcgcaac cgggcggcat cgtcgtgccc
     4441 gcttaccggg gcaaacgcgg ccacccggtc ggtttcggca gcagcttccg tgccgagttg
     4501 ctcggcctgg aaggagacga aggcgcacgc tccgtggtcg agcgtcacgc gcagactgtc
     4561 gagaccatcg actgcgacga tcccgggatc ctgcgcgata tcgacacgcc gggcgacgcg
     4621 ctctgaggcc gatcaattcg gtttgaggac gatggccttg agattgccgt cgacgatgaa
     4681 gttgccgcgg atctgcgccg cctgcgatct cagttcgacg aatacgcgcc cgtgtacttc
     4741 cttgttcggc aatacttcga ggccggcggt tgccgagagc agccctgagg cgagctttaa
     4801 attgcgatac tggtagcggc caccggcgac agtgagcgcg ccggagattt cctcgaaccg
     4861 ggtcgcccca ccctgcacgt tttgcctccc ccccatctgc agcgcgcgca ccaggtccac
     4921 accgtccagg tctcccttcc tggttacgaa cgtaccttca atcctgggcg tatcgaacag
     4981 cgtgacgagg tcgggagcgg ccagcgagta acgcagtgtg gcttccaggt gcccggttga
     5041 cttcgcatcc cgcgtgaaca ccgacatcaa ttcctgaagc gcgacgcgct ctgaatttac
     5101 ctggccctcg atcgaccacg gcccgcccca attgatcaat gccgaaccgt tcgcggcgcc
     5161 gccgtaaagg agggcccgga attcggcgac ccggatctga tggcccgagg ccactgcctt
     5221 ggccgagaaa tcggtgaact cgatctgtgg gccgatcgga ggcacccagc ccttgccggc
     5281 cgagaccgcg atatcgagtt cctcgccgcg cggcgtgatc tgcactgaaa gcttgccgtc
     5341 ggtcgtttcc aatgccgctt tccggacggc gccctccggg gaaagatcga gctccgcatc
     5401 gaagttcggc accttcaccg tacgcgattc cagcttcacg cctttgaata ccacacgctt
     5461 gacctgcaag ttcttgtcgg taaccgccgc ctccatccac ttgggcaaac gcggcagcac
     5521 ctcctgagcc acgctggccg actcgaccgt gacgttcctg atgatcttga cttcatcgaa
     5581 caccgacccg agatccagcg acagatcgac cttgtcgatc ttgacgtcct gcgtggtgcc
     5641 gagctttacg ttgtcgaggc tcatcgagaa tccaccgagc accgatacac gcaccgagcc
     5701 gattgttacc ggttccccga tatggccgga cgccagtctc tcgatcgacg gaatataggt
     5761 gttcatcggt atcaactgga taccgccgac caccagtgcg acgacgacga cgactgctat
     5821 gatggccggc ctgaagcgct ccaggggcgt gcgaatcccc ttgtgctttt tcagggattg
     5881 gacttcggcg cgcttgcgat cgcgttcgtc ctgttccttg cgccggcgcg ccaactcctc
     5941 ctcgcgcgcc ctgcgatcgg attcgatctt ggcctgctcc tggcgttcgc gctccagccc
     6001 ggccttgacc ttgcgctctt cctgctcctt gatccggagc tctttctctt cgttgcgctt
     6061 gcgctcgcgc tcttcccggt cctggcgttc gcgctccatg cgcccgcgcc gctcacggtc
     6121 ctcggcttca cgccgttccg cctccagcct cgcttcttct tcgcgctcgg cacgctcgat
     6181 tgcgtgacgc gactcttcct gctcgcggtt acgagtctct tcttcctgct cgcggcggcg
     6241 caattcttcc tgctgcttct cgaactccga ggcaatctgg ctttccatcg atctcaggcc
     6301 cgagaggtcg aattccggaa gatcgttttt cgcggccgcc tcgctgcgcg cacgctcctc
     6361 gtcctcacgc cgcttgcgct cttcttcctc gcgtctgcgc cgttggtctt cttccgcgcg
     6421 cctgcggtcc tcctcttcgc ggcgcaggcg ccgctcctcc tcttcgcgcc tgccgcgttg
     6481 ttcctcttct tcccgtttct tgcgttcttc ttcttcgcgg cgcttgcgtt gctcttcctc
     6541 cttgagtttg cgttcctctt ccgcacgtcg cttgcgctct tcttcctcct tgcgcttgcg
     6601 ttcctcttcg tccctgcgct tgcgctcttc ttcctcgcgg cgacgccgtt cctcgtctac
     6661 tttgcgccgg ccctcctcct cttccttgcg cttacgctcc tcttcttcgc ggccgcggtg
     6721 ttcttcgacc tcgcgagtct tgtgttcctc ttcttccctg cgctggcgtt gcgcgtcctc
     6781 gcgattgcgc cgctcggcat tctcctgcgc gtcgcgctcg aatgcctcct gttttgtccg
     6841 atcggactgt tccagtgcct tgcgctcggc ttcgaggatc tttcccaggt cccgttgcgt
     6901 ctgcgccaac gcctcggctt cgagctttgc cttgcgctcc gcttcctcgc gcaatttgag
     6961 cgcggcggct tccttggcct tgcgctcggc ctcgaccttg gcaaggcgcg ccgcttcgtc
     7021 gcgggcctgg cgcagcgcct gctctttttt caggcgctcc tcttcctcgc gcttcttttt
     7081 ggcttcctcc tccgccttga cgcgatcggc ggactcgcgc tggcggaatt cggtctgcgc
     7141 cgactggtag ggattctttc ccggagccag cgaggaggtg aagtcgagat cgtcgacgta
     7201 cgagctttcc agtggggtgc tggatgcgct gccggaggtg ttggaaaact ccttgatata
     7261 cccgccggtg gtcaactgca acagcgagcg atctatgtcg gcatcgctga gtttggactt
     7321 ggcgatgagc tctgcgacag tggacttgcc gtcgatcagc gtcaggacct tgacgaggtc
     7381 cctggcaagc ttgccggctt tgttcttgat ctccagtacc cctttgccgg tcttggagta
     7441 gatcgtcttc ttgtccatcc tgttctttct cagtccccgg gaatgaagaa atacaacaac
     7501 ggactcatag accagacatc gagcggggaa gcctggaacg gcgaggcggc aaaaacaaca
     7561 acgactctcc cccaagaaat accgttgttt cggtaattat taggcctgac ggcgcgcgcg
     7621 tctagtgtcc tgttctgctg attcgtggga taactcgtga accttggacg ttagtcccat
     7681 gatctgtttg acagcaatcc atcatcgatc ggaaaacccg cgaattagcg gaacaggaca
     7741 ccaaggtcaa atcgccttga cgatgtcctc cacgaccttc ttggcgtccc cgaagaccat
     7801 catggtcttg tccatgtaga agaggtcgtt gtcgaggccg gcgtagcccg ccgccatgct
     7861 gcgcttgacc accagcaccg tacgcgccct gtgtacttcc agaatgggca ttccgtatat
     7921 cgggctgccg ggcacgttgg cggccgggtt gaccacgtcg ttggcgccca ggaccagtac
     7981 cacgtcggtg gtcgggaatt cgttgttgat ctcttccatc tcgaatacct ggtcgtaggg
     8041 aatttccgct tccgccagca gcacgttcat gtgtccgggc atgcgtccgg ccaccgggtg
     8101 gattgcaaaa cggactttca cgcccttttc gtgcagcttt tccgccattt ccttcaccgc
     8161 gtgctgggcg cgggccactg ccagtccgta gcccggaacg atgatgaccg actcggcgtt
     8221 gcccagcagg aaagccgcgt cctccgggct gccgctgcga tgagtcttct gcgccacgtc
     8281 gccggtcgct gccgcggtat cgccgccgcc gaagccaccg agaatcacgt tgaagaacga
     8341 cctattcatc gccttgcaca tgatgtagga caggatcgcg ccggagcttc ccaccagcga
     8401 accggaaatg atcaacatcg gattgttgag cgaaaaaccg atgccggccg ccgcccagcc
     8461 ggaatagctg ttgagcatcg aaatcaccac cggcatgtct gccccgccga tcgggatgat
     8521 gatcagcacg cccagcacga acgcgatcgt ggtcatgatc gcgaacggcg tccagctttg
     8581 attgaacatg aaggcgacgc caaaagcgat catgacgatt gccagcacca gattgaccca
     8641 gtgctgtccg ccgaacacca ccggggcgct gcgtatcctg ccggagagct tgccgaaggc
     8701 gatcacggag ccgctgaagg tgatggctcc gacgaaggtg ccgatgaaaa gttcaagccg
     8761 gtttccgcgt ggaatgacgc cgtcagcggt caggattccg taggctccgg gattatggac
     8821 cgcggcgaag gcgatcagca ccgcggccag acccaccagc gagtgcatcg ccgccacgag
     8881 ctccggcatc gccgtcatct ggatgcgccg cgcgacgacc gcgccgacgc taccacctac
     8941 cgcaacaccg agcacaatca gcatccagtt ctgcgtgacc gacagcgtgg tgagcacggc
     9001 gatcaccatg ccgataatgc cgaacagatt gcccctgcgc gccatcagcg gtgacgacag
     9061 tcccttgagc gacaggacga aaaacaccga cgcgaccagg tatgccagcg ccactagatt
     9121 tgcagacatt cagttctccg ggacgggttc gtttccgtgg atcttcttgt ggctacgacg
     9181 taacgcgccc atggcgcatc agtctccgcc gcaagctcac ctaggttcgc ttgtcttttt
     9241 tcttgaacat ctccagcatg cgctgggtca ccaggaatcc gccgaaaacg ttgacgcttg
     9301 cgagcaccac ggcgacgaag cccagcaccg tgccccagtc gatcggttgc gggccggccg
     9361 ccagcatggc gccgacaatg atgatgccgg agatggcatt ggtcaccgac atgagtggcg
     9421 tatgcagcgc cggagtcacg gtccacacca cgtgatagcc gacgaaaatg gccagcacga
     9481 acacggtcag atggttaacg atcggatcaa tggcttccat gtctctctcc aatggtgagg
     9541 cgtgaggtga aaggcgtgag gaccgcgatg cggcgatatc cccttcaccg ttcacccttc
     9601 actctttact gtttcttgac cggctccccg ccgatacaca ccagcgatcc ggcgatgatc
     9661 tcgtcctcgc ggttcagttg cagctgcccg gtcttggggt ccagcaacag gttgaggaag
     9721 ttcagcaggt tgcgcgcgta cagcgcgctg gcatccgcag gcaccagcgc cggcaaattg
     9781 gcgatgccga tgatttgcac gccgtgtttg accacggtct tgtccagttc cgagagcggg
     9841 cagttgccgc cctgctccac cgccatgtcc acgatcaccg agcccggctt catggtcttg
     9901 accatatcct cggtgaccag caggggcgcg ggacggccgg gaatcagtgc agtggtgatc
     9961 acaatgtccg cctgcttgat gcgttccgcg atcagggccg cctggcgctt cttgtagtca
    10021 tcgcccatct cgcgcgcata accgccctgg ccctttgcca gttctttctc ggcctcggta
    10081 agcggcacct cgacgaattt ggcgcccagc gactcgacct gttccttggt ctcgggccgc
    10141 acgtcggtgg cttccactac tgcgcccagg cgcttggcgg tcgcaatcgc ctgcaggccc
    10201 gccacaccca cgcccatgat caccacccgc gccgccttga tcgtgccggc agcagtcatc
    10261 atcatcggca tcaggcgctg gtaggcgttc gcggccagca tcactgcctt gtagccgccg
    10321 acgttggcct gcgaggacag cacatccatg ctttgtgccc gcgaaatccg cggcagcgat
    10381 tccattgcaa acgcggtgac gccggtcctg gcgatggctt cgatcccctc cttcttgtaa
    10441 ggcgcgagca gcgcgatcag gacgccgccg gacttgagca tggcgagttc gcccggttcg
    10501 ggaccacgta ctttcagcac gatgtcgcaa ccataggcct cggccgcact gtgcgccacg
    10561 gcagcaccgg cggccgcgaa ctcggagtca ggtatcgaac agccggcgcc cgcgccgctc
    10621 tggaccagca acttatgatg gccggagccg gcaagtttct tgacggtttc cggggtcacg
    10681 gcgacccgcg tttcgcccgc gcggatttcc gccggtatgc cgatctgcat ggatggaaaa
    10741 acctccctgt cgagctggcc tccggcggaa cgatgcgctc gtcatggagg ccaaggtggg
    10801 tgtcacaaaa gtgcgcaatt ataagtgata ggcgagacgc gaggcgaggc acatcgcggc
    10861 aacacaccga tcggccaccg ggtcacgccc gccagggcct gactcgggcg tgcgccctga
    10921 acccaaatgc ccggggctcc gagccggggc ctgcggctat aatcgctctg accccacaga
    10981 cctgcatcga tgcccgccat ccacctgctt ccggacctcc tgatcagcca gatcgcggct
    11041 ggggaagtgg tcgatcgccc cgcctccgcg ctcaaggagt tgctggaaaa cagcctggac
    11101 tcgggcgcta ccgacgtgac tgtgcagctt gccgaaggcg gcatcaggca gatccgcgtc
    11161 gcggacaacg gcgcaggcat cggcaaggac gacctggcgc tcgcgcttgc ccgacacgcc
    11221 accagcaaaa tcgcttcgct cgaggagctg gagcgcgttg ccagcctggg attccgcggc
    11281 gaagcgctgg ccagcattgc ggccgtttcc catctcagcc tgctgagccg gaccagcgac
    11341 agcccgcatg cctggaggtt ggatgcgagc ggcggcgcgt tgtccgcagc cgagcccgcc
    11401 gccgcggttg ccggcaccac ggtcgaagtg cgcgaccttt acttcaacac tcctgcccgg
    11461 cgcaaattcc tcaggagtcc cgcaaccgaa tttgcccaca gcgaagagag tttctggcgc
    11521 atcgctctgt cgcgcccgga tgtacgcttc acgctcacgc acaacggccg cgcgcagtcg
    11581 cacctgcatc cagcaggctc cgaggagcgc atacacgcag tactgggaga ggccttccgc
    11641 gaagcagcga tcccggtcca ggcagaggcc ggcagctttc gcctgcgcgg tctggtcgcc
    11701 caaccgacct actctcgcgg ggcccgcgac gcgcagtact tcttcgtcaa cggccgttac
    11761 gttcgggaca agttgctggc gcacgcggtg cgccaggcct atcacgacgt gcttcatcac
    11821 gagcgccatc ctgcctacgt gctgtttatc gacctggacc cggcgctggt ggatgcgaat
    11881 gttcacccta cgaagatcga ggtgcgtttc agggattcgc gcggcataca ccagttcgtt
    11941 tttcacgctc tcgagaaggc gctggccggg acccgtgcgg gaaatccacc tgcgcaggag
    12001 gtcggcgccg cgatggcctt tccgcgcgcg ggcacatccg cctttcgcca gtcgggaatg
    12061 gacctgcgcg ccgcgcaacc ggcggcgttc tatgcaacgc tgttcggagc gggccaggcc
    12121 acactggagg caccggcacg cgcacctgcg gcgctgccgc aggatgcgcc gcatccgctt
    12181 ggattcgccc tggcgcagct ggccggaatc tacatcctgg cgcagaacga cgagggcctg
    12241 gtggttgtgg acatgcatgc cgcccacgag cgcatcgttt acgagcagct caagagcgcg
    12301 ctcgacgttg accgcatccc gacgcagcag ttgctgattc cggccacctt gacggcgagc
    12361 gcgctcgagg tggccactgt cgaagaccac gcgcagacct tgtccaggct gggcttcgat
    12421 ctcgcggcca tcgcaccaaa tgcgatcgcg gtacgcgcgg taccgatgac gcttgcccag
    12481 gccgactccg ctgaactcgc ccgcgacgtg ctgagggaaa tcgcgcaatt cggcggcagc
    12541 cgggtgctcg ccgagcgtca gaacgaaatg ctttccacca tggcctgcca cgctgcagtc
    12601 cgggccaacc gttcgctcac cgtggccgag atgaacgcac tgctgcgcga catggaagcg
    12661 accgagcgct ccggccagtg caaccacggg cggccgacct ggttccaggt cacgataggc
    12721 gaactggaca ggatgttcat gagaggaaag tgaggagtga ggggtcaggg gtgaggggca
    12781 agagcttttc gtccctgtat acccaattct tctctcgtcg cctccctcag gacgcaatgg
    12841 cgacaggcct gccgagcgca agcgtggaga gttagtgccc tcctccttac ccctcacccc
    12901 tcacccctca ccacaggacc gccctgaggc aatcctgctc atgggcccca ccgccagcgg
    12961 caagaccggg gtggcgctgg aactggcgcg gcattttccg gtggagatcg tcagcgtcga
    13021 ttcggcgctg gtgtaccgcc gcatggacat cggtaccgcc aagccggatc tggcgacccg
    13081 gctcgaggtg ccgcaccatc tgatcgacat catcgatccc accggcaatt attcggcggc
    13141 gcagttttgc gatgacgcgc tggaggcgat gcgcggcatc gtcgaacgca accgcgtgcc
    13201 gctgctcacc ggcggcacca tgctctattt caaggcgctg caggaggggc tttccgacct
    13261 gccggcggcg gatgcggata tccgcatggt gatcgacgcg atggccgtga aggccggctg
    13321 gcccgccata catcgcgagc tcgagcgcat cgatccggcc accgccgaac ggctcgaccc
    13381 gaacgatgcg cagcgcatcc agcgcgcgat ggagattttt tacctgaccg gcaagccgat
    13441 gtccgagctg atcgccgcgg gcaggagcgc aagcctgccc tatcgcatga ttccgatcgc
    13501 gctgattccg ggggaccgcg cggctctgca tcgacgcatc gccgcgcgtt tcgaagagat
    13561 gctggaactg gggttgatca acgaggtgcg cgcactgcgc gaggactacg acctcaagcc
    13621 ctcactgccg tcgatgcgtt gcgtaggcta ccggcaggtg tggcaatacc tcgatggcga
    13681 attcggcctg gcggcgttgc gcgacaaggc tgttgccgcg acgcggcaac tggcgaaacg
    13741 ccagctcacc tggctgcgtg caataccggg ggtcaaagcg tttgacggtc tggacgaggg
    13801 gttgaagggg cacatcgagg cctggcttcc ggaacaactc gggcggtaag gaccgggagc
    13861 gttgcgcacg ctcccggtaa accggaaatc agttggcgag cgaatccgcg ccggccttcg
    13921 cgatctggcc gtcctcggtg gatttcacgc cgcttacgcc gatggcgccg accagttgcc
    13981 caccaaccat gatcggcagg ccgccttcca acggcaaggc tccatgcaat gcgatcaacg
    14041 cattgcggcc gccggcaatc gcatcttcga ataccttggt cgggcgcttg aaagcctgcg
    14101 cggtgcgcgc cttctggatg gccacctcga tgctgccggt ttgagtgccg tcgatgcgtt
    14161 gcagatagac cagatgtcca ccgtcgtcca ccacggcgat gactacgttc cagttgttct
    14221 tcctggcctc ggcttccgca gccgcggcaa ttttcttcgc accttcgagt gtaagaacct
    14281 ttttgtcggc aagctgggcg ttcgcgttca tcgacagcgc cgcaaacacg gccaaagcga
    14341 ccagggaaag cattctcttc acgagcgaca taactcctcc tcattattga tctggttgat
    14401 tgatcctgcc cggctacggc ggtcgagcac aggcaggctt gctcgacagg cgaataatgg
    14461 ctgccgtgct aaagataccc cttccctccc gcatgccgca atcagtccac gtcttccttg
    14521 ctctcgacgc gcgccagggc ggtgccgcgc gcaaacgcca gacgcagccg ttcactggtc
    14581 tgcacctcag ccgccgagcg cacgatacgg ccgtcaacct tggcgacgat gctgtagcca
    14641 cgctctagga ccgaccgcgg attgaggtgc tcgaggtttg cacccagcct gcgcagccgc
    14701 acttcgtgaa gctccagcag gcgggcgtaa ccgagtttca ggcgccgcgc cagttcccga
    14761 tgctgcagtg cagccgcaac gagatccggc cgcgtcgtac gcaatctgcc caggagatcg
    14821 gcaacacgcc aagacgccgt gtccagcact ttcatcatgg acaatttgag ccgtgtcgcc
    14881 agatgcgcga gatgccgggc ctggtcgcgg atgcgctgcc ccgggtgaac cagacgtttc
    14941 cccagatagt cgagctgctg catgcgcgct tccagcagcc gatcggcact gtgtttcaat
    15001 tgccggtaca gcgcgccgag gcgtaccagc aactcttccc ggttagggct cgccagctct
    15061 gcggcagcgg tcggcgtcgg cgcccgcgcg tcggcaacaa aatcggcgat ggtgaaatcg
    15121 gtttcgtgcc cgattcccgc tatgaccggg atcgcgcaat cggcgatcgc gcgcgccacc
    15181 acttcctcgt tgaacgccca caagtcttcg atgctgccgc caccgcggca gatgatcaat
    15241 acgtccgcat ccgcgcgcgc cgaggcggag cggatggcct gcgcgatcct ctcgcccgcg
    15301 ccctcgccct gcaccggcgc cggatacagc acgatgcgaa tcgccggcat gcgccgcgca
    15361 agcgtggtaa gcacgtcgcg aagcgccgcc gctttgggag aggtaacgat gccgatgacc
    15421 agggggaaag cgggaatcgg cttcttgcgg gaagtctcga acaggccctc ctgctcgagt
    15481 tttttcttca gccgttcgaa cgcctcgaac agggcaccca gcccggagcg gcgcatggtc
    15541 tcgacgttga gctgaaaatc tccgcgcgcc tcgtacagcg tgggagccgc gcgcacttcg
    15601 acctgcatgc cgttctcggg ctgccagtcc agcgccgagg ccttgtggcg gaagaacacg
    15661 cagcgagcct gcgcctgtgc gtccttcaga aggaaatagc aatggccgga ggtcgcgcgc
    15721 gtgaagttcg agatttcgcc ggcgacccag aggagcggaa aaccggactc gaccagcgtg
    15781 cgcgcgcgcc ggttgagttc cgagacgctg atgacgatgt tcgccggatc ctgcgagatc
    15841 atgtaccgca ttctatgcga gcagggctga tccgtagagt tctgcccggc gcatacttcg
    15901 gtgcgcctgc cgtttcgtcg gaaatttttc tcgccgattc agcctgtttt cgactctcga
    15961 caacgcgcgc cgggcaccgc tgttcattca caatggtcac aaaacggtca cgcgagtacc
    16021 ccggaaaccg cgcaaccacg cggccctcga atacaagccg ttgaataaaa tacggaaaac
    16081 ggggcgcata ttttttaatc gccgaacaaa aaatgctttc gccaactcca cttaccttct
    16141 ctttgtgcaa accatccaca gtgttatcca caaagattgt ggacagtttt tcgcgccgtg
    16201 aatgcgcttt gccgaagctg tagagtgcga tgaggcaaac ttgcccgtga tggcgatgca
    16261 gtttaaagtg tcgcgatttt tctgacgcaa tggagactac gtgctagcca tcatcgaagc
    16321 agccggttgg ccaatctggc ccatcatcct gtgctcggtc gtcgcggtcg gcatcatcgg
    16381 cgagcgcttc tggtccttgc gccaggacat tgtcgcgccg cgtggactgc tggcgcaggt
    16441 ggtgcaggaa taccgccaga agggtgtgtc gccggagatg ctggaccggc ttgccttgtc
    16501 gtcgttgatg ggcaggatcc tggccgcggc gctgcgcaac gtcggcagca cgcgcgagat
    16561 catgcgcgaa tcgatcgaag aggcgggacg cgccgccgca catgacctcg agcgttttct
    16621 cacgacgctc ggcaccatcg ccaccatgtc gccgctgctc ggactgctcg gcaccatcat
    16681 cggcatgatc gagatcttcg gagtccaggg cccgaccggg atggccaatc cagcccaact
    16741 cgcgcacggc atttcaattg cgttgtacaa cgccgcgttc ggcatcatcg tcgccgtccc
    16801 cagcctggtg ttctaccgcc acttccgcgc gcgggtggat tcgctggtgg tggaaatgga
    16861 aatccaggcc gtcaagctgg tcgagatcat ccagggcgag cgcaaggcct gagctgacaa
    16921 gggaggcacg cgatgaactt ccgccgtggt tcgttccagg aagagcccga aatcaatctg
    16981 gtgcccttca tcgacgtact gatcgtcatc gtcatcttcc tcgccgtcac cacgacgtat
    17041 tccaagtatt cggaactgca gatcaacctg cccacggccg atgccaatcg gccggccgaa
    17101 cgtccaaacc aggtcaacgt cggcgtatcg gcctcaggcc agtacatggt caatcgcaca
    17161 ccggtgacgt tcacgacccg ggaggcattc gccaacgagt tgcggcgcgc cgccagcggc
    17221 aatcccgatc cggtgatcat catcaacgcc gacgccaacg ccacgcatca gtcggtgatc
    17281 catgtcatgg aagccgcaca gatggcgggc ttcggcaaaa tcacctttac cgcgcagttg
    17341 ccgcgctgac ctcaggctca tattacgcac cgcggtacca ttgcacctga tctccgggga
    17401 gctcgcgtgc ccttttccga tcgccattgg tatcgcctgt cctggctttc cgtcgccctg
    17461 ttgccgcttg cggcactgtt ccggctctcg gcgagtttgc gcaggctcgc ctacaggatc
    17521 ggcgtactgc gctcggagcg tctgccggtg ccggtcatcg tggtcggcaa tatcaccgcc
    17581 ggcggcaccg gcaagacgcc gctgacgctg tggctgtgcg gcatcctccg gcaacaggga
    17641 ttcaagcccg gcatcgtcag ccgtggctac ggcgggctcg ccaggctgca ggccgtaacc
    17701 gcggactccg atccggccgc cgccggtgac gagccggtgc tgctggctcg ccgcagccgc
    17761 tgcccggtct ggatcgggcg cgacagggtt gccgcggcgc gcgcgctgct ggccgcaaat
    17821 cccgagtgcg acgtgatcgt ctgcgacgac gggctgcagc actaccgctt gcggcgcgac
    17881 atcgaggttg tcgtcctcga cggcagccga ggtctgggca acgggctgcc gctcccggcc
    17941 ggcccgctgc gcgaaggcgc ggcgcggctg cgatcggtgg atgcggttgt gatcaacggg
    18001 ccgggcggtt tcggtgtgcg cgtcgggtcg cgtttcgaca tgtccctcga aggcagctac
    18061 ttccacaacc tgctcaatcc cggccatacc gcagccgcgt cggaattcca cggcaggcgc
    18121 ctccatgccg tggccggcat cggcaatccg ttgcgattct tcgagcatct gcaacggttg
    18181 gggttgagtt tcaccgcgca tgtcttcccg gatcatcatc cgttcgcgcc cgccgatctc
    18241 gacttcgagg atgcggacga gatcatcatg accgagaagg atgcgataaa atgccaacgc
    18301 ttcgcacggg aaaaccagtg ggcactgccg gtggacgccg ccgtcgatcc cgcacttggc
    18361 caacacatcc tcgacagact gaaatcgcgc catggatcct aagctcctcg acatcctggt
    18421 ttgccccctg tgcaagggtc cgctcgtcta ccggaaggag gagcaggagc tcatttgcaa
    18481 ggcctgccgg ctcgcttacc cgatccgcga cgacattccg gtgatgctgg aagacgaggc
    18541 gcgcaaactt cttcctacgg aagaagtttg agtgacgagt tacgcgtgac gtgtgacgcg
    18601 cgagtcaatc ttcgacgggt cctgaacaca ctcgtcacct gtcaccggtc acatgaactt
    18661 tgtcgtcgtc atccccgccc gttacgcttc ttcgcggctg cccggcaagg cgctcgccga
    18721 gatcgccggc aagccgatgg tcgtgcatgt cgccgatcgc gctcgcgaat cgggtgcggc
    18781 cgaagtcatc gtggctaccg atgacggcag gatcctggac gcggtcggcc gtcatggcca
    18841 cgccgcgatg atgacgcgcg cggagcacgc ctctggcacc gaccgcatcg ccgaggtcgc
    18901 ggtccagcgc ggttggtccg ggcagcagat catcgtcaac gtccagggcg acgagccgcg
    18961 tattgctccg gcgctgatcc gtgccgtcgc acaacagctc gccggtcatg ccgatgcggc
    19021 gatcgccaca gtctgtcacg cgatccatga tgcagcagaa atgttcgatc cgaacgcggt
    19081 aaaggtagtg atcgacaagg acggctacgc gctgtacttc tcgcgcgcac cgatcccgta
    19141 tgctcgcgat gccttcgggc cggaccggtc gcgatggccg gccgggctgc cggcgtatcg
    19201 ccacctgggc ctctacgctt atcgctgcgg cttcttgagg acttattcga cgctcgcgcc
    19261 cgcagcgatc gagcagttcg aatcgctgga acaactgcgc gccctgtggc acggccaccg
    19321 catcagcgtg ctgatcaccg atcaggcgcc ggaacccgga gtggatacgc ccgcggacct
    19381 ggaaaagatg cgcaaggcct tcgcaggcgc cgcgcgctga atcaaccctt ataattcgcg
    19441 ctgcgcggca agttgctacg gaaagcgtgg cccgccgtca cttggcgctc gactctctac
    19501 aacaggaatg acatgcgact tattctcctt ggcgcacccg gcgcgggcaa aggcactcag
    19561 gccgccttca tcagcgagaa gttcggcatt ccccagattt ccaccggcga catgctgcgc
    19621 gccgcggtca aggccggcac tcccctgggc cggaaagcga agaaattcat ggacgccggc
    19681 gcgctggtgc cggatgacct catgatcgga ttggtcaagg agcgcatcaa gcagccggat
    19741 tccgccaagg gtttcctgtt cgacggtttc ccgcgcacca tcccgcaggc cgatgccatg
    19801 aagaacgcag gcgtgaatct cgactacgtg gtcgaaatgg acgtggacga cgccgagatc
    19861 atcaagcggc tgtccggccg ccgcgtgcat cccgcatccg ggcgtaccta tcacgtggcg
    19921 ttcaacccgc cgcgcgcccc cggcaaggac gacgccaccg gcgaagacct gatccagcgc
    19981 gacgacgaca aggaagaaac cgtgaagaag cgtctcgagg tgtaccactc gcagaccaag
    20041 ccgctggtcg cgtactactc gaagtgggcg gcaagcggca aacccggcgc accgaaatac
    20101 gtcaagatcg ccggcgtcgg ctcgatggat gccatccgcg accggatctt cgccgctttg
    20161 aaatgacaaa tcttcaccgc agaggcgcaa aggcgcagag gaaacgcaga gaaaaccaaa
    20221 ggaagcgtgt caaaaagcgg gggggccgat ttgtcccacc cagtcgatat atcgcggtgg
    20281 cgcgactatc aacctctttg ctttttaacc gaaagtgttt ttgttttcct ttgcgtttcc
    20341 tttgcgcctc tgcggtgaag atctaaaggg gtagatacca tggcaaagag aaacgcgttc
    20401 tatgcgcagt caggcggggt aaccgccgtc atcaatgcat ccgcgtgcgg cgtcatcgag
    20461 accgcgcgca atcacaagaa caggatcggc aaggtctacg ccggtcgcaa cggcatcatc
    20521 ggcgcgctca ccgaagacct gatcgacacc ggcagggaat cggcttcggc catcgcggcg
    20581 ttgcgccaca cgccggccgg tgcattcggc tcctgccgct acaagctcaa gggtctcgac
    20641 gagaaccgcg cccagtacca gcgcctgatc gacgtgttca aggcccacga catcggctac
    20701 ttcttctaca acggcggtgg cgattccgcc gacacctgcc tgaaggtgtc gcagctttcc
    20761 gagaccctag gctatccaat ccaggcgatt cacgtgccca agaccgtgga caacgacctg
    20821 ccgatcaccg actgctgtcc cggtttcggt tcggtggcaa agtacgtcgc ggtgtcggtg
    20881 cgcgaagcca gcttcgacgt ggcgagcatg gcgaagactt cgaccaaggt gttcgtcctg
    20941 gaagtcatgg gtcgtcacgc cggctggatc gcggcagcgg gcgcattggc ggccgatgcg
    21001 aaaaccgacc tgccgatcat catcctgttt ccggaagttg ctttcgacaa ggcgaagttt
    21061 ctcgccagag tggattccac ggtgaagaaa tacggttact gctcggtggt ggtctcggag
    21121 ggcgtaaaag gcgcggacgg caagttcctc tccgaccagg gactgcgcga cgccttcggc
    21181 cacgcgcaac tcggcggcgt tgcgccggtg gtggcggaca tggtcaggga aggactgaat
    21241 ctgaaattcc attggggggt ggccgactat ctgcagcgcg ccgcccgcca catcgcttcg
    21301 ggcaccgatg tcgcgcaggc ctacgcggta ggcaaggcgg cagtggagct tgcgctcaaa
    21361 ggaaggaacg cggtcatgcc gaccatcgtg cgcaaatcgc agaagcctta caaatgggtc
    21421 ataggcgtgg ctgagctgaa ggaggtggcg aacaaggaga agttcatgcc gcgcgatttc
    21481 atcagcgaag acggcttcca gatcacgcag aaatgccgcg actatctcgc gccgctgatc
    21541 cggggcgagg atttcccgcc tttcaaggac gggcttcccg gctacgtgat cctcaagaac
    21601 gtcgcggtac cgaagaaact gcccgcgttc aaactctgaa tcccgccgcc gaccgttcaa
    21661 aacggtcaat ctgcagggaa atttcggcta aaatcgcgcg ttgaccggcc gcccccttcc
    21721 caagattacc ggcagggagc gatgcggcgt tcgttcattt tagggtcaag gggccggccc
    21781 gtgattcagc ccagaaatta taaacagata aagggggact catgtcttca gcactgatct
    21841 tcgcgctggt gtgcgcgatc atcgggatca tttacggtgc gatctcgatc aagtggatac
    21901 tgggcaggcc tgccggcaac gcgcgcatgc aggaaatcgc gcgtgccatc cagcaaggcg
    21961 cccaagccta cttgaatcga cagtacacca ccatcgccat cgtcggtgca gtgctgttct
    22021 tcgtgctcgg cttcgggctc gactggtata ccgcgatcgg cttcatcgtc ggcgcagtcc
    22081 tctccggtgc caccggttac atcggcatgt tcatttctgt gcgcgccaac gtgcgcactg
    22141 ccgaggccgc caatcacggc atcaatgccg cgctgaacgt ggcgttccgc ggcggcgcga
    22201 tcaccggcat gctggtggtg ggactgggac tcctgggcgt ggccggttac tacgcggcaa
    22261 tcctgcctac cgcaggcgca gccaaggcgc tgcacgcgct ggtgggcctg gccttcggcg
    22321 gatcgctgat ctcgatcttc gcgcgtcttg gcggcggcat cttcaccaag ggcgccgacg
    22381 tcggcgccga cctggtgggc aaggtcgaag ccggaattcc ggaagacgac ccccgcaacc
    22441 ccgcggtgat cgcggacaac gtcggcgaca acgtcggcga ctgcgccggc atggcggccg
    22501 acctgttcga aacctacgcg gtcaccatcg tcgccaccat gctgctgggc ggcctgatgg
    22561 tggaatcggt gggcgcgaac ggcatcctct atccgctggt tctgggcggc gcttcgatca
    22621 tcgcttcgat cgtcggcacc ttcttcgtga agacttccga ggggggcaag atcatgaacg
    22681 cgctgtacaa gggcgtaatc gtctcgggcg tgctcgccgc gattgccttt tacttcatca
    22741 ccacgaaaat gatggccgac agtccgatcg cagccgtgaa cctgttctat gcctcgctgg
    22801 tgggtctggt gctgactgcg gcgctggtgg tcatcaccga gtactacacc gccaccgaat
    22861 atgcaccggt gcgccacgtc gcggccgcgt cgcagactgg ccatgccacc aacatcattg
    22921 ccggcctcgg tgtttcgatg aaagcgaccg cattgccggt gctggcggtg tgcgcgtcga
    22981 tctgggcctc gtactcactg gccggcctgt atggcattgc cattgccgcc accgcgatgc
    23041 tgtcgatgac cggcatggtc gtggcgctgg acgcctacgg tccgatcacc gacaacgccg
    23101 gcggcatcgc cgagatggcc gggctgccgt cgaagatc
//
LOCUS       DASQOP010000101        23421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1849948, whole genome shotgun sequence.
ACCESSION   DASQOP010000101 DASQOP010000000
VERSION     DASQOP010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 23421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23421
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1849948"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..1176)
                     /locus_tag="VE030_04085"
     CDS             complement(202..1176)
                     /locus_tag="VE030_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HZF22630.1"
                     /translation="MTKAVRFHRTGGPEVLQVDDVQVGEPGDGQARVKHTAIGLNFID
                     TYHRSGLYPLPLPSGIGLEAAGVVEAVGQGVTHVKPGDRVGYAGGPPGAYSEVRLVPA
                     DRLVKVPSGISDKEVAAMMLKGLTVQYLIRRVYHVEKGETVLLHAAAGGVGLIACQWL
                     KALGATVIGTVGSDEKAKIAKAHGCDHTIVYTRESFAERVKQITGGAKVPVVYDSVGK
                     DTFMGSLDCLKPRGLLAMFGNGSGPVSAFDLNLLAAKGSLYVTRPTLMTYTARREDLE
                     AGAKELFDIVKSGKVKIEINQTYKLEDAAQAHRDLEARKTTGSTVLLP"
     gene            complement(1293..2789)
                     /locus_tag="VE030_04090"
     CDS             complement(1293..2789)
                     /locus_tag="VE030_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HZF22631.1"
                     /translation="MEHLIQAFELVFEPYVLMVIGASALYGLFVGAIPGLTATMATAL
                     LVPVTFFMPPVPAVAAIVTATAMAIFSGDIPGALLRIPGTPASAAYCDEAYAMTRKGQ
                     AELVLGAGLVFSVIGGIFGTIVLVTAAPTLANVALNFSSFEYFWLVLLGLTCAIFIAS
                     NNVLKAFVSLLIGLLIAAVGMENPAAFPRFSFGEPNLMGGISLIPMMVGLFAVSEVLR
                     YATSIELPLHLAQEKFGNIFKGQWALVRQYPVQTLRGSALGTLIGALPGAGADIAAWM
                     SYAMSKKFSKQPEKFGTGHVEGVIESGAANNSALSGAWIPALVFGIPGDSITAIAIGV
                     LYLKNMNPGPMIFINDPQNIYAVFIVFFLANLLMLPLGVLAIKSFKQVLKVPRAVLMP
                     VILLFCTVGAFAINNSPFDIGVMLAFGVLAFVMEENGFPVAPVILGAVLGRMLEENFV
                     TSLIKADGHFVAFFSRPIAGVLGSVTIAVWVSPLVIRWWRGRVRSVTD"
     gene            complement(2794..3267)
                     /locus_tag="VE030_04095"
     CDS             complement(2794..3267)
                     /locus_tag="VE030_04095"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HZF22632.1"
                     /translation="MKFNDSLVGAGLIVFAGAILWNAWGFPSMPGDQVGPALFPQLIA
                     AGLAICGAIFVIQAARRKADTPWIELPDWIRARRQVAGFAAVTLGLLAYCLLVEALGF
                     FVCAPLLLGALLAVLRVRLWAVPVIAIGVSVLIHFIFYKGLGVPLPWGLLQSWAW"
     gene            complement(3351..4277)
                     /locus_tag="VE030_04100"
     CDS             complement(3351..4277)
                     /locus_tag="VE030_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZF22633.1"
                     /translation="MVAANAVLAAGFPSRPVTLIVPWGAGGGTDAVARMLASLLEKDF
                     GQPVNVVNRTGGSGVVGHQAIATAAPDGYTFGLITFEITQMHHQGLTQLNGASFTPIG
                     LINVDPAAIQVNADSPYKTLPDLLDAIRKNPGKLKAAGTARGGSWHLSLYGMLADLKI
                     DPASVPWVPSVSNAAGLQELAAGGVDIVSGSHPEARALIEAGRVRSLAIMDEAPSQLF
                     PRVPTLKAAAGSKWLSGVWRGIAAPKNLPKAIEERLIAAVKQAYDSKEYREFMTGRGF
                     GMRYLPPQEFAALMAKTDAENGVLMKKFGMTQ"
     gene            4337..4594
                     /locus_tag="VE030_04105"
     CDS             4337..4594
                     /locus_tag="VE030_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22634.1"
                     /translation="MVHKLAPAANNSPADASHLIFLMEQPNRAAARRRTGLAGALFSG
                     WTEFYCKRDDMHQKSLAETLIDKPLQALTARDAKDARGSQM"
     gene            complement(4765..6018)
                     /gene="phaZ"
                     /locus_tag="VE030_04110"
     CDS             complement(4765..6018)
                     /gene="phaZ"
                     /locus_tag="VE030_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HZF22635.1"
                     /translation="MIYQLHEFQKTVMAPTVLLADTVKHLFTSPFSPLAYTAVSRTIA
                     ANSEVFVRLMRRYPKPQWRIDDTMIGDKCVAVTREVVVDKPFCQLLHFRKDIAVGQPK
                     LLIVAPLSGHFATLLRDTVRTALPDHDVWVTDWVDAKMVPLSKGAFHLDDYVDYIREF
                     IRHLSPDLNVMSVCQPTVPVLAAVSLMAAENDPASPKAMIMMGGPIDTRRNPTAVNDF
                     ASGRPHSWFANKVIMRVPPNYPGFMRRVYPGFLQHASFLAMNPDRHLDAHKQFYDHLI
                     RGDGESAEVHRQFYDEYNAVLDMPAEYYLDTVKTVFQEFQLPKGRMTVRGVPVKPETI
                     RKTALFTIEGELDDISGNGQTEAAHTLCRSIPADKRKHLLAIGVGHYGIFSGRKFREL
                     VYPQIRSFIRLIAQREEDLKLVRVK"
     gene            complement(6149..7135)
                     /locus_tag="VE030_04115"
     CDS             complement(6149..7135)
                     /locus_tag="VE030_04115"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HZF22636.1"
                     /translation="MSETTPPYAGLTPDLMLNAVESVGFHCDGRLLALNSYENRVYQV
                     GIEDDKPLVAKFYRPARWSDAQILEEHTFTAELAEREIPAVPPIAVGTRTLHAFQEFR
                     FALYPRRGGRAPELDDPDTLEWLGRFIGRIHAVGALSSFVHRPALDIATFGEEPREYL
                     LGNDFVPADLCAPYAAVIDQALAGVRGCFERAGTVRSIRLHGDCHPGNVLWTDDGPHF
                     VDFDDARMGPAVQDLWMLLSGDRAAMTRQLSDVLAGYEDFFEFDRRELHLLEALRTLR
                     MIHYSAWLARRWNDPAFPAAFPWFHTTKYWQDQILQLREQVAAMQDSPLGPL"
     gene            7469..8521
                     /locus_tag="VE030_04120"
     CDS             7469..8521
                     /locus_tag="VE030_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZF22637.1"
                     /translation="MKSVTCKSLLTAALACLWAGAAIADDESIKAKIPALVTEHMTIP
                     SDTPGIHLYIRNRRPKDLIVSPDRILLYVHGATYPSETAFDLRLDGMSWMDYIAAHGW
                     DVYLVDLRGYGLSTRPPEMDRPPQDNPPIVTTGVAVRDVATAAEFIRKRRGVAKISLL
                     GWSWGTSIMGAYAAANNDKINKLVLYAPLWLRNTPSLIGADGPLGAYRAVTRENARKR
                     WLTGVPEDKQADLIPAGWFDAWAEATWGTDPKAKDSGQLRAPNGVIQDGREYWAAGKP
                     YYDPANIRATTLLIKAEWDQDTPAYMSQALFPLLKNAPAKRYVEIGEGTHTVIMEKNR
                     MQLFREVQLFLDEPSR"
     gene            complement(8730..9779)
                     /locus_tag="VE030_04125"
     CDS             complement(8730..9779)
                     /locus_tag="VE030_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22638.1"
                     /translation="MKTLPDPRAYTPISSNPLVLAAERSANPGSALQQEDAGGMLLSR
                     FRKALEVGDDARIEQAFAEATSQSVRETLTRALGSALEARDEAALVMRVFAIPLLIVT
                     GGRSLAVVPGVVPDVGELRKLFEKQGALGRSKNFGLGNALTTAEKLAAVKPSMLYRLA
                     RGSGQEGFRPPDLAPQDIEVDSGEECVHLRFLVGAAVAPANAPGFTETAGNIGAWGLP
                     FTQALAAQLGQQGLSLLPIPRPPMNLQRALETGRFALREMEFQLFLGNALRRFRARVG
                     DPDATVASYSDGSVRISLGSPFDASLACEYRWPLYPEDDLGAVGTSIFGLLADCRVET
                     VQVAETVWLAKAASH"
     gene            9980..11485
                     /locus_tag="VE030_04130"
     CDS             9980..11485
                     /locus_tag="VE030_04130"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZF22639.1"
                     /translation="MWLSDEERKRTSTADDAAFASPVPTQIVSNGEYNPMPQTEQQRQ
                     VEGRIKELADQYAAKLNMDRRRFLKTASGMATAFLAMNQVFGPIWDVSEAEAADIDMA
                     DARAKALKGQFIFDDQTHFVRDDFDKEGLLGLGKYASEHWNPDMLKDIPLVLQRYKFQ
                     NYLKEIYLDSDTKVALLSGAPFDDPDWWLLSNDQIIQARAAINKVAGSRRLFGHTVIT
                     PGQDGWMDEVDRGIAELKPDAWKCYTIGDPLSPSTKGTAWRLDDEGLVYPWYEKAMKS
                     GIRTICIHKGLLPLDYETSWPGVWKHATPDDVLKAAKDWPQLNFIIYHGAMRNFLEDP
                     AYSLDKFEKTGRIDWTTDVCELASKHGCKNVYAELGTTFATVAVASPRLAAAMIGQMV
                     NQMGPDHVVWGSDSVWYGSPQWQIEAFRRLEVPDDIMKKHGWKTTLGGPDSKVKRMIF
                     GENSARIYKYKIQAEYPALGHDQLALMKEQYETEGVERNNVAYGFVGKKTA"
     gene            11641..11952
                     /locus_tag="VE030_04135"
     CDS             11641..11952
                     /locus_tag="VE030_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HZF22640.1"
                     /translation="MNDPAARCPQEPGVFRPVIDRNRCEGKEDCVAVCPYQVFSMGIV
                     PPELRRGLTLIGKLKGYAHKWHQSFALNADACHACGLCVAACPEKAITLGREPVNHAR
                     A"
     gene            11956..12771
                     /locus_tag="VE030_04140"
     CDS             11956..12771
                     /locus_tag="VE030_04140"
                     /inference="COORDINATES: protein motif:HMM:NF017672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006672 - ceramide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ceramidase domain-containing protein"
                     /protein_id="HZF22641.1"
                     /translation="MARTAGIWFLALLALAVTVAALALPPLAQPPEYHQFADQRTLLG
                     VPNFFNVFSNVAFLLVGGAGVALLLRNRLPHASRPLLDRSVRWAYLVLFAAVALTCIG
                     SAYYHLAPDNSRLTWDRLPMSVAFMALLAAMISERIHPSAGVTLLGPLVLVGIASVAY
                     WHWGEAAGTGNLRPYVAVQTFAIVAVLLIVFLFPPRYSRGTDILVAVALYAAAKGAEF
                     YDGEIFSAGRLVGGHALKHLFAALAVFWILRMIWKRAPVTGPAAANISSASAS"
     gene            13086..13220
                     /locus_tag="VE030_04145"
     CDS             13086..13220
                     /locus_tag="VE030_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22642.1"
                     /translation="MISIPANVAHSAEAVERLVAYDILTPPRQDWITGADAYLRSTAE
                     "
     gene            complement(13314..14246)
                     /locus_tag="VE030_04150"
     CDS             complement(13314..14246)
                     /locus_tag="VE030_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22643.1"
                     /translation="MHERKHTHALSGLSLIISHGLITLLAIGIALTLPVVAQFILYQW
                     WPRVEADSTLLFTTEIAFASVLVLLFNLAKTAWDNRRYVANAKLASLAYAKSNYNWLT
                     RWQEKALFKRLPVTRDACILTVTGFDTFARKSSRFSTVLQTAYEIRVMLLNPASRGAR
                     LRVDSMPPERSDFGSFCEEVEASISYLSALRRTGRKVTLKFYDHQPFWKVVVLGDHVW
                     VQYCHGGCEIKQTPEYVFALHHRNPTRGLFVPFYMYFLEKWDEHHHPEFDFDTRELVY
                     RDAMGQESGRRPFSIPPVYGTDAANEQQLAARAG"
     gene            complement(14653..15129)
                     /locus_tag="VE030_04155"
     CDS             complement(14653..15129)
                     /locus_tag="VE030_04155"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZF22644.1"
                     /translation="MKHLFVSLALVLPLVAHAGFDDAVQAYGTGDYAKALAEFRVLAD
                     QGKPEAQYFMGLFYHNGFGVPRDQNESARWFLKAARQGDARSQYYVGIMAEKGQGVEK
                     DPVAAHMWLSLSASNPQTSHRDSLYTREAIEKLEKKMTPEQVARAKETAKNWNPQN"
     gene            15597..15845
                     /locus_tag="VE030_04160"
     CDS             15597..15845
                     /locus_tag="VE030_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014631734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HZF22645.1"
                     /translation="MLQLRPNCECCDKDLPPDSTEAMICSFECTFCRGCAIGVLQGVC
                     PNCGGELVRRPIRPQGKLARNPASSERVLKPGGCRHVA"
     gene            16113..19952
                     /locus_tag="VE030_04165"
     CDS             16113..19952
                     /locus_tag="VE030_04165"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HZF22646.1"
                     /translation="MSAILTRISGVPYIAKVGVLAVCYFAAAKASLLFAIPPGYATAV
                     WPPAGIALAALLLHGVRIWPGVWLGAVLANFSVNQSLTLAAAIATGNSFEAVCAAILV
                     NRLIESPLEFRQPHVVFRFALIAAASSLVAATSGVGALFASGSMPARNFLDNWYTWWQ
                     GDTTAIIVVTPLLLAWLREARRDEPPRRTEVIVFGGLLVLAFVAVFVFARWTRPGIGH
                     ALPFLLIPFMAWAGCRFDERIVTAAVFAVTILAIWGTVNNLGPFASADLNESLLSLQA
                     FTGTVTLMSLVLCALTRERAGAAAALQRSHDRLELAVSERTAELADNNRQLARDVAER
                     ERLTLVLQRREAQLAQAQAITHTGSWTWDLRTDRVTWSDELFRIYDLSPERFTESFEA
                     YLSRVHPQDRERVQEVIRKALADHQPWELTERIVRPDGVIRVLRSTGNVLTGEDGAAI
                     VMHGVCVDATEQIRLERIQAVQNGIARAMVQEQVPADALVAALRVICVTLGWDVGAMW
                     KLDPERGVLHHAHSWHPIEKDASVFVTASRQMLFKRGHGLPGRIWEQEKAVWIEDVFD
                     DSNFPRAGFVTQAGLRAAFGFPLIAGHRILGVMEFFGAHAQKPDDELLEMIAAVGNQL
                     GEYIVRNEAQVLLTESEERFRLLVERVKDYAIFMLDPEGRIASWNSGAQRIKGYRTDE
                     IVGKHFSCFYPPEDIASGQPERALEIARAAGRYEAEAWRVRNDGSRFLANVVITPLRA
                     DDGGLRGYAEVTRDITQSRRSEDAMRHSETMLRSVLDTLPVGVWMIDCKGGIVEANPA
                     AQRIWAGSRYVGMDEYGVYKGWWAGTDRLIQPHEWAGARAVERGETSINELVDIECFD
                     GTRKTIVNSAVPLRDAEGRIAGAIIVNEDVTARRQAEQALTGVAAGVSAATGAEFFRL
                     LVEQVTQALGADGAIIGELTGKKEGEVRVVAGLMDGRMVDSFTYDCHGSPCAIVLTEN
                     AVVHADDARARFSHTPVLDRYSADSYFGIGLWGADGGTLGVMMVINRKPLRNPELAKS
                     ILRIVASRAAAELERKRNEETIRSLAIRLQEMIEEERTRIAREIHDELGQALTGLKMD
                     LSWLSKSVDKRDSKVMAQIKSMAEFIDSLIHSVRRISTDLRPGELDDLGLVAAIESQA
                     QDFESRTGIKCRLSLPEADLAVDPGIATAVFRICQELLTNVARHSGATRVSLDLECND
                     AWLSLAVQDDGRGIRVAEASSRNAIGLAGIRERAKMFGGSVTIKGKPGKGTLARVRIP
                     IGRARDAANGNDDVAAA"
     gene            20004..20633
                     /locus_tag="VE030_04170"
     CDS             20004..20633
                     /locus_tag="VE030_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003278556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZF22647.1"
                     /translation="MKVLIADDHAVVRRGLRQILEAEPDLEVAGEATSGTEVMEALRM
                     QRYDVAVLDITMPGRGGLDVLHDIRQNFPELRVLILSMHPEERYALRVLKAGAAGYLT
                     KESAADELITALRKVAAGGRYVSPTLAERLADEIGIGIDQPLHDRLSDREHQILCMIA
                     NGMTVSGIASELHLGTNTVSTYRARLLGKLKLANNAEITHYAIRNRLVD"
     gene            20820..21254
                     /locus_tag="VE030_04175"
     CDS             20820..21254
                     /locus_tag="VE030_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZF22648.1"
                     /translation="MPAGLQSMKVVIVDDSALLRQRLAAMISRLSGVEIAGEAKSVAQ
                     ALELIRRCHPEAVMLDIRLKDGNGFDVLRQVKREPGAPVVIMLTSFPSTKARQTCQAL
                     GGDFFFDKATEFERAIEVLTDLEFARRTSKSSEFNRPAGAGC"
     gene            21789..>23421
                     /locus_tag="VE030_04180"
     CDS             21789..>23421
                     /locus_tag="VE030_04180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZF22649.1"
                     /translation="MKKEFRRLASDSNRGTGDAAADAFPTGSGKEIRILFVEDVISDF
                     ELTIRELKQAGVACAVHRVDTREGLLRELKDFEPDVILSDFSLPTFDGLSVLMIVREL
                     RPDIPCIFVSGTISEDQLISLVRHGAADYVLKSNLERIPPAVLRAVQERNERAARRQA
                     EQALKESEALLDSVFDNIPSMLFIKDAKDLRFVRFNRTGEELLGRSEKDLLGKNDYDF
                     FPKEQADLFVAKDRETLASNQRVFIMEEKIEVKDGTERMLQTKKLAILDEQGNPRFLL
                     GISEDVTERKQAEQQIRDSEAKYRGLIEQASDGIFVSDAEGNLVLANSRLCELLGYAK
                     DEVTGLNARQTYLEEERETHAKLLEQVRAGQTLRYERTVQRKDGSHFPAEISLKMLDN
                     GLIQGIFHDITTRRAQEHKIARLSRIQAVLSGINSAIVRIRDRQTLFDEVCRLSIEHG
                     GFALAWIRMTTPDAQGGRPIAWRCSNDSYLDLIPLSIETDAGRAHAERVMSEKRAVVY
                     NDLTQAGDFPFAGQALQAGALSLAKLPLVVSGEAVG"
BASE COUNT         4748 a         7098 c         7177 g         4398 t
ORIGIN      
        1 cttcagatcc tcaatcattt tccttcccca gtttaatttt caccacagag acacagaggc
       61 gcggagaaaa ccatttcgac aggaaatcat cgggaatatc agcgcacaaa taaggattcg
      121 atcgattcct gatggtacgc cacgcgtgct cgacgacaat tctgtttttc ctccgtgtct
      181 ctgtgtctct gtggtggttt ctcaaggcaa caggacggtc gagccggtgg tcttgcgggc
      241 ttcgagatcg cgatgcgcct gggctgcgtc ctcgagcttg taggtctgat tgatttcgat
      301 cttgaccttg ccggacttga cgatgtcgaa caactccttc gcccccgctt cgaggtcttc
      361 gcgacgggcg gtgtaggtca tcagcgtggg gcgagtgacg tagagcgagc ctttcgccgc
      421 gagcaggttg aggtcgaacg cggaaacggg accggagccg ttgccgaaca tggccagcag
      481 gccgcgcggc ttcaggcagt cgagcgagcc catgaaagta tccttgccca ccgagtcgta
      541 cacgacgggc actttggcgc cgccggtgat ctgcttcacc cgctcggcga agctctcgcg
      601 ggtgtagacg atggtgtggt cgcagccatg ggctttggcg atcttcgcct tctcgtccga
      661 cccgacggtg ccgatcaccg tcgcgcccaa tgccttgagc cactggcagg cgatcagacc
      721 gacaccgccg gccgcggcat gcaacagcac agtctcgcct ttctccacgt ggtacacgcg
      781 gcggatcagg tactgaacgg tgagcccctt gagcatcatc gccgcgactt ccttgtcgga
      841 gatgccgctg ggcaccttca ccaggcggtc agccggaacc aaacggactt cggaatacgc
      901 cccgggcggc ccgcccgcgt aacccacgcg atcgcccggc ttcacgtgag tcacgccttg
      961 gccgacggct tcgaccacgc ctgccgcttc caggccgatg ccgctcggca gcggcagcgg
     1021 atacaggccg gaacgatgat aagtgtcgat gaaattcaag ccgatggcgg tgtgcttgac
     1081 ccgggcctgg ccgtcgccgg gttcgccgac ctggacgtcg tcgacctgaa gaacttcggg
     1141 cccgcctgtt ctgtggaaac gtacggcttt ggtcatgatt ttctccagaa tttccttgtt
     1201 agttatctct ttccgtcggg ctatgccgcc ctccagcatg ctccgatatt ctatcagcgg
     1261 catcgtcgat cagatgcctg tcgcccaatc tcttagtcag taacggaccg cacgcgaccg
     1321 cgccaccacc ggatcacgag cggcgaaacc cataccgcga tggtgaccga tccgagcacc
     1381 ccggcgatcg gccgactgaa gaatgcaaca aagtgcccgt ccgccttgat caatgaagtg
     1441 acgaagttct cttccagcat gcggccgagc acggcgccga gaatgaccgg ggccaccgga
     1501 aaaccgttct cctccatgac gaacgccagg acgccgaatg ccagcataac gcctatgtcg
     1561 aagggggaat tgttgatcgc aaacgcgccg accgtgcaga acagcagaat cacgggcatc
     1621 agcaccgcac gcggaacctt gagcacctgt ttgaagctct tgatcgccag cacgccgagc
     1681 ggcagcatca gcagattcgc aaggaagaac acgatgaaca cagcatagat gttttgcggg
     1741 tcgttgatga atatcatcgg gccgggattc atgttcttca ggtacagcac gccgatggcg
     1801 atggcggtga tggaatcgcc gggtatcccg aatacgagcg cgggaatcca cgcgccggac
     1861 agcgcgctgt tgttcgccgc cccgctttcg atcacacctt cgacgtggcc ggtgccgaac
     1921 ttctccggct gcttcgagaa tttcttcgac atcgcatacg acatccaggc cgcgatgtca
     1981 gcgcccgcgc ccggcaacgc cccgatcagt gtgcccagcg cgctgccgcg cagggtctgc
     2041 accggatatt ggcgcaccag cgcccactgt cccttgaaga tgttgccgaa cttctcctgg
     2101 gcgaggtgca gcggcagttc gatcgatgtc gcatagcgca ggacttcgga cacggcgaac
     2161 aatcccacca tcatcgggat caggctgatc ccacccatca gattcggttc gccgaagctg
     2221 aagcgcggaa acgcggccgg gttctccatc ccgacggcag cgatcagcaa gccgatcagc
     2281 agcgatacga atgccttgag cacgttgttc gaggcgatga agatcgcgca ggtcagtccc
     2341 agcagcacca gccagaagta ttcgaaggaa ctgaaattca gggcgacgtt ggcgagtgtc
     2401 ggggccgcgg tcaccagtac gatggtgcca aaaataccgc cgatgaccga gaacaccagg
     2461 ccggcaccga gcaccagctc ggcctggccc ttgcgggtca tggcataggc ctcatcgcag
     2521 tacgcggcag aagcgggcgt gccgggaatg cgcaaaagcg cgccgggaat gtcgccggaa
     2581 aaaatcgcca tcgcggttgc cgtgacgatc gccgcgaccg ccggcaccgg cggcatgaag
     2641 aatgtgaccg gcaccagcag cgcggtcgcc atggtggcgg taaggccggg gatcgcgccg
     2701 acgaacagcc catacagcgc cgatgccccg atcaccatca gaacataggg ttcgaagacc
     2761 agctcgaaag cctggatcag atgttccatg tccttaccag gcccatgact gcagcaggcc
     2821 ccatggcaaa ggcacgccca gtcccttgta gaagatgaaa tggatcaaca ccgagacgcc
     2881 gattgcgatc accggcaccg cccagagccg aacgcgcaga accgcgagca gcgccccgag
     2941 cagcagtggc gcgcagacaa agaaaccgag tgcctcgacc aggaggcagt acgccagaag
     3001 acccaacgtg acggctgcga aacccgccac ctgccgccgc gcgcgaatcc aatccggcag
     3061 ttcgatccag ggcgtgtccg ccttgcggcg cgccgcctga atgacgaaga tcgcaccgca
     3121 gatcgccagc ccggccgcga tgagttgcgg gaacagcgcg ggaccgacct ggtcgccggg
     3181 catcgacggg aatccccatg cattccagag aatcgcgcct gcaaacacga tcaggccggc
     3241 gccgacgagg gagtcgttga atttcatcgc gaggctagag gctagaggct aagaattgga
     3301 cgcgggtcgt cattggcggt tcgattttgc cctctcgcct ctcgccttgc ttattgcgtc
     3361 attccgaatt tcttcatgag cacgccgttc tcggcgtcgg tcttcgccat caatgccgcg
     3421 aattcctgcg gcggaagata acgcatcccg aaaccgcgcc cggtcatgaa ttcccggtac
     3481 tccttgctat cgtaggcctg cttgactgcg gcgatcagcc gttcctcgat cgcctttggc
     3541 aggtttttcg gcgcggcgat cccgcgccag acgccactca gccatttgct gcccgctgcc
     3601 gccttcagcg taggcaccct gggaaaaagc tgggacggcg cttcatccat gatggccagg
     3661 ctgcggaccc tgcccgcttc gatcaatgca cgggcttcgg gatgggagcc gctcacgatg
     3721 tccacgccgc cggcagccaa ctcctgtaac ccagccgcgt tgctgacgct cggtacccaa
     3781 ggcacggagg ccggatcgat cttcaggtcg gccagcatgc cgtaaagaga cagatgccag
     3841 gaccctccgc gagcggtgcc ggcggccttg agcttgcccg ggttcttgcg tatcgcgtcg
     3901 agcaagtccg gcagcgtctt gtagggggaa tcggcattaa cctggatcgc cgcggggtcg
     3961 acgttgatca acccgatcgg cgtgaagctc gcgccattga gctgggtcag tccctgatgg
     4021 tgcatctggg tgatctcgaa ggtgatcagg ccgaacgtat aaccgtcggg cgcggctgtg
     4081 gcgatcgcct ggtgtccgac cacgccgctg cccccggtgc gattgaccac gttcaccggc
     4141 tggccgaaat ccttctccag cagggaggcc agcatccgcg ccaccgcgtc ggttccgccg
     4201 cctgcgcccc atggcacgat cagggtgacc ggccgcgacg gaaagccggc ggcgagaacc
     4261 gcgtttgcgg caaccataaa cgccgttgca gcaacgacag ccagaaactt cttcattttc
     4321 tcctcgccag gtgtgcatgg tgcacaagct tgctccggcg gcaaacaact cgccggcgga
     4381 tgcttcgcac ctgatttttc tgatggaaca gccgaatcgc gccgcggcgc ggagacgcac
     4441 cggcctggcc ggtgccctgt tttcaggatg gacggaattc tattgcaaaa gagacgacat
     4501 gcaccaaaaa agcttggcgg aaacactcat cgacaaaccc ttacaagcgt taaccgcaag
     4561 ggacgccaag gacgccaggg gaagccaaat gtaagaataa agtgggtgtt caaacccagt
     4621 gcaagtatct gcgttgcgac tgacgcatta ccctgattca gcctggcctt cgcgtccttg
     4681 gcgtccctag cggtgaatgc gttaatgctg aggcgttcga aacccacaga aaagtctgaa
     4741 gaacgtcgcc tccgccatgc ggacttactt cacgcgcacc agcttcaggt cctcttccct
     4801 ctgggcaatc aggcggatga agctgcgtat ctgcggatag acgagctcgc ggaacttgcg
     4861 tccgctgaag atgccgtaat gacccacacc tatcgccagc agatgcttgc gcttgtcagc
     4921 ggggatgctg cggcacaggg tatgcgcagc ttcggtctgg ccgttgccgg aaatgtcgtc
     4981 cagctcacct tcgatggtga acaatgccgt ctttctgatc gtctcgggtt tcaccggcac
     5041 gccgcgcacc gtcatcctgc ctttcggcaa ctggaattcc tggaacaccg tcttgacggt
     5101 gtcgaggtag tactcggcgg gcatgtcgag caccgcgttg tattcgtcgt agaactgacg
     5161 atgcacctcg gcgctctcgc cgtcgccgcg gatcaggtgg tcatagaact gcttgtgcgc
     5221 atccaggtga cggtccggat tcatggcaag gaaactggcg tgttgcagga agcccggata
     5281 caccctgcgc atgaagcccg gataattcgg cggcacgcgc atgatcacct tgttggcaaa
     5341 ccacgagtgc ggacggcccg aggcaaaatc gttcaccgcg gtcgggttgc ggcgggtatc
     5401 gatcggcccg cccatcatga tcatcgcctt cggtgacgcc gggtcgttct ccgccgccat
     5461 cagcgacacc gcggcaagca ccggcacggt cggctggcac accgacatca cgttgagatc
     5521 cggcgacagg tgccggatga attccctgat gtagtccacg taatcgtcga ggtggaacgc
     5581 gcccttcgac agcggcacca ttttggcgtc cacccagtcg gtgacccaga catcgtggtc
     5641 cggcagcgcg gtgcgcaccg tatcgcgcag cagcgtggcg aagtgcccgg aaaggggagc
     5701 gacgatcagc agcttcggtt gcccgacggc gatgtctttt ctgaaatgca gcagttggca
     5761 aaacggcttg tccacgacaa cctcgcgggt aaccgcgacg cacttgtcgc cgatcatcgt
     5821 gtcgtcaatc cgccactgcg gtttcggata acgccgcatc agccgcacga agacttcgct
     5881 gtttgcagcg atggtgcggg aaaccgcggt atacgccagc ggactgaagg gactggtgaa
     5941 gagatgtttg acggtgtccg cgaggaggac cgtgggcgcc atcaccgtct tttgaaactc
     6001 gtgcagctga tagatcatcc tgcctcgccg agaggtttgc ggctgcgccc ggatttctcc
     6061 atgtgaaatg ccgtgccggc agcctgccgc ggaatgtaaa gacaatgtca cgaagcgtca
     6121 agaacgccgt cacattggaa gtttccgcct aaagcggccc cagcggcgaa tcctgcatcg
     6181 ccgctacctg ctcccggagc tgcaatatct ggtcctgcca gtatttcgtg gtatggaacc
     6241 acgggaaagc ggccgggaag gccgggtcgt tccagcgccg cgccagccat gccgagtaat
     6301 ggatcattcg cagcgtgcgc agcgcctcga gcagatgcag ttcgcggcgg tcgaattcga
     6361 agaagtcctc gtagccggca aggacgtcgg aaagctggcg ggtcatcgcc gcgcggtctc
     6421 cggagagcag catccacagg tcctgaacag ctggccccat gcgcgcatcg tcgaaatcca
     6481 cgaaatgcgg tccgtcgtct gtccacaaga cgttgcccgg atggcagtcg ccgtgtaatc
     6541 ggatcgatcg caccgtgccg gcgcgctcga agcagccgcg cacaccggcg agcgcctggt
     6601 cgatgacggc tgcgtacggc gcacacaggt cggcgggaac gaaatcgttg cccaacagat
     6661 attcgcgcgg ctcttcgccg aaggtggcga tatccagggc gggacgatgc acgaacgacg
     6721 aaagcgcccc cactgcgtgg atgcgcccga tgaagcggcc gagccactcg agcgtgtcgg
     6781 gatcgtccag ttccggggcg cgcccgccgc ggcgcggata cagcgcgaaa cgaaattcct
     6841 gaaaagcgtg caaggtgcgc gtgccgactg caatcggcgg cacagcggga atctcgcgtt
     6901 ccgccagttc cgcggtgaat gtgtgctcct cgaggatctg cgcatcgctc cagcgcgccg
     6961 ggcggtagaa cttcgcgacc aggggtttgt cgtcttcgat gccgacctgg taaacccggt
     7021 tctcatagct gttcagcgcc agcagcctgc cgtcgcagtg aaagcccacg gattcgaccg
     7081 cgttcagcat cagatcgggc gtcaacccgg catatggtgg tgtggtttcg ctcatgcccg
     7141 gacgatacgc gaactctgcc gacagcagaa ccctcaatgc tcaccgcaaa gacgcaaagg
     7201 cgcaaaggta acgcagagaa aatctagaaa gcatgaatag agcaaaagac gtagcgggtg
     7261 actttgcgct gatgaatgga ccgtacccgt catgtccctt cctgcgccga ttcttttttt
     7321 gcccaggcaa gttctacttt gcgttacctt tgcgcctttg cgtctttgcg gtgaagaaca
     7381 tggttttcgc agtagacggg gtgacgcgcg cagccgcttt cgctacagtg gcagccggcg
     7441 ccgatagagc gcgcacaagg agatcaagtt gaagtccgtc acctgcaaat cactgctgac
     7501 tgcagcgctg gcatgtctgt gggccggagc cgcgatcgct gacgacgaaa gcatcaaggc
     7561 aaaaataccg gctctcgtca ctgagcacat gactatccct tccgacacgc cgggaatcca
     7621 tctctatatc cgcaacaggc ggccgaagga tctgattgtg tccccggacc ggatcctgct
     7681 gtacgtgcac ggggccacct atccgtccga aaccgcgttc gatctgcggc tggacggcat
     7741 gtcgtggatg gattacatcg ccgcgcacgg ctgggatgtc tatctcgtgg atctgcgcgg
     7801 ctacggactt tcgacgcgcc cgcccgagat ggaccggcca ccgcaggaca acccgccgat
     7861 agtcactacc ggcgtggcgg tgcgcgacgt tgcaacggcg gcggagttca tccgcaagcg
     7921 ccgcggcgtg gcgaaaatca gtctgctcgg ctggtcgtgg ggcacgtcga tcatgggcgc
     7981 gtatgctgcg gcgaacaacg acaagatcaa caagctggtg ctgtatgcgc cgctgtggct
     8041 gcgcaacacg ccgagcctga tcggcgccga cgggccgctg ggggcgtatc gcgcggtaac
     8101 gagggaaaac gccagaaaac gctggctcac cggcgttccc gaggacaaac aggcggatct
     8161 catcccggcg ggctggttcg acgcctgggc ggaggcaacc tggggaaccg atccgaaggc
     8221 gaaggacagc ggacagttac gcgcgcccaa tggcgtgatt caggacgggc gcgagtactg
     8281 ggccgcgggc aagccctatt acgatcccgc gaacattcgc gcgactacgc tgctgatcaa
     8341 ggcagagtgg gaccaggaca cgccggccta catgtcacag gcgctgttcc cgctgctgaa
     8401 aaacgcaccg gcgaagcgct atgtggaaat cggcgaagga acacacacgg tgatcatgga
     8461 aaagaaccgc atgcagcttt tccgcgaagt ccaactcttc ctcgacgaac cctcaagata
     8521 aataaatctc aacgcaaaga cgcaaaggac gcaaagaaaa ccacgcaaaa ggaagcaaac
     8581 ttgagagaac aatatccgct ctgaatgctg ccgccggttg ccaacgacga ggcacgacat
     8641 acgcagcccg ctcggtttga gctcccattt tcctcgtttt ccttagcgat tcctttgcgt
     8701 ctttgcgcct ttgcggtgaa aaggtctggt caatgcgaag cagccttggc gagccagacg
     8761 gtttcggcga cctggacggt ttccaccctg caatcggcca gcagaccgaa gatgctggtc
     8821 cccaccgcgc cgagatcgtc ctccgggtag agcggccagc ggtactcgca ggccagcgac
     8881 gcatcgaaag gcgagccgag gctgatgcgg acactgccgt ccgagtagga ggcaacggtc
     8941 gcatccgggt cgccgacgcg cgcgcgaaat cggcgcagcg cattgccgag aaacaactga
     9001 aattccattt ccctcagcgc gaagcggccg gtttccagcg cgcgctgcag gttcatcggc
     9061 gggcgcggga taggcagcag cgacaaacct tgctggccga gctgcgcggc caacgcctgg
     9121 gtgaacggca gcccccaggc gccgatgttg cccgcggttt cggtaaagcc cggtgcgttt
     9181 gcaggcgcaa ccgccgcccc gaccagaaag cgcaggtgta cgcactcttc tccggagtcc
     9241 acttcgatgt cctgcggggc aaggtccggc ggccggaatc cttcctgtcc cgatccgcgg
     9301 gcaaggcggt aaagcatgct gggcttcacc gcggccagtt tctcggcggt agtcagcgcg
     9361 ttgcccagtc cgaagttctt cgaccggccg agcgcgccct gtttttcgaa cagtttccgc
     9421 aactcgccga cgtccggcac gaccccgggc accaccgcca ggctgcgccc gccggtgacg
     9481 atcaacaacg gaatggcgaa tacccgcatc accaacgcgg cctcgtcgcg tgcttccagc
     9541 gccgacccga gcgcacgcgt gagcgtctct cgcaccgatt gggaggtcgc ttccgcgaag
     9601 gcctgctcga tccgggcgtc gtcgcccact tccagggctt ttcgaaagcg gctcaggagc
     9661 attcccccgg catcttcttg ctgcaacgca gaacctggat tcgccgagcg ttcggcggcc
     9721 aagaccagcg gattcgatga gatcggcgtg taagcccgtg gatcaggaag ggttttcatc
     9781 agcagaaact ttgatgacgg ccattgtaac aacccgtgcg caggcaaccg tgccgtggcg
     9841 cagcgacgac ggtacttgac tccgttcaat ttaagactag aaagagaggg gtggccaaag
     9901 gtgtaataag tggaaacacg ccgatggctg ccatcctgaa cgttcagaac aatccagcct
     9961 tcagaagggg gagtcccgta tgtggttaag tgacgaagaa cgtaaacgca cctcgacagc
    10021 cgacgatgcc gcgtttgcat cgccggtgcc gacacagatt gtttccaacg gcgaatacaa
    10081 cccgatgccg cagacggagc agcagcgtca ggtggaaggt cgcatcaaag aactcgccga
    10141 tcagtacgca gccaagctca acatggaccg gcgccgcttc ctcaagacgg cttcgggcat
    10201 ggcgaccgca ttcctggcca tgaaccaggt cttcggtccg atctgggacg tttccgaggc
    10261 ggaggccgcc gatatcgata tggccgatgc acgtgccaag gcgctcaaag ggcagttcat
    10321 ctttgacgat cagacgcact tcgtgcgcga cgatttcgac aaggaaggcc tgctgggtct
    10381 gggcaaatat gcttccgagc actggaatcc ggacatgctg aaggacattc cgctggtcct
    10441 gcagcgctac aagttccaga actaccttaa agagatctac ctggacagcg acaccaaggt
    10501 ggcgttgctt tcgggtgctc ccttcgacga tccggactgg tggctgctca gtaacgacca
    10561 gatcatccag gcgcgcgcgg cgatcaacaa ggttgccggc tcccgccgcc tgttcggcca
    10621 cacggtcatt acgcccggac aggacggttg gatggacgaa gtcgaccgcg gcatcgccga
    10681 actgaagccg gatgcatgga agtgctacac gatcggggat cccctgtcgc cgtccaccaa
    10741 aggcaccgcc tggcgcctgg atgacgaggg cctggtctat ccgtggtacg agaaggcaat
    10801 gaagtcgggc atcaggacca tctgtatcca caagggcctg ctgccgctcg actacgaaac
    10861 gtcctggccc ggcgtatgga aacatgccac cccggacgac gtgctcaagg ccgcgaagga
    10921 ctggccgcag ctcaacttca tcatctacca cggggcgatg cgcaacttcc tcgaggatcc
    10981 ggcatattcg ctcgacaagt tcgaaaagac cggccgcatc gactggacca ccgacgtgtg
    11041 cgaacttgcc tcgaagcacg gctgcaagaa cgtctacgcc gaacttggta cgacgttcgc
    11101 aaccgttgcc gtggccagcc cgcgtctggc ggccgcgatg atcgggcaga tggtcaatca
    11161 gatgggcccg gaccacgtgg tctgggggtc ggattcggtg tggtacggca gcccgcagtg
    11221 gcagatcgag gccttccgcc gtctggaagt accggacgac atcatgaaga agcacggctg
    11281 gaagactacg ctcggcggtc cggacagcaa ggtcaagcgc atgatcttcg gcgaaaactc
    11341 tgcgaggatt tacaagtaca agatccaggc cgagtacccg gctctcggcc acgaccaact
    11401 ggccctcatg aaggagcaat acgaaaccga gggcgtagag cgcaacaacg tggcttatgg
    11461 gttcgtagga aagaaaacgg cgtagaaagt cacagtcgaa acccgacggg gagcgcaggc
    11521 tccccgtttt tttgcccgct tgtcttgcgt ggctgccacc gtgttgccgc tttgcacgcc
    11581 ccggagcggc aggatagggc atgattgcag aagctaactt gattcagaca cgaccaaacc
    11641 atgaatgacc cggcagcacg gtgcccgcag gaacccggag tcttccggcc ggtcatcgac
    11701 cgcaaccgct gcgaaggcaa ggaagactgc gtcgccgtgt gtccgtatca ggttttcagc
    11761 atgggcattg ttccgcccga actacggcgc ggtctcacgc tgatcggaaa actcaagggc
    11821 tacgctcaca aatggcatca atcgttcgcc ttgaacgcgg acgcctgcca tgcatgcgga
    11881 ctatgtgtgg ccgcctgccc ggaaaaagcg ataacgctcg gccgcgaacc ggtgaaccac
    11941 gcaagggcct gagtagtggc caggacagcg ggcatctggt tcctcgcttt gctggcattg
    12001 gcggtaacgg tcgccgccct ggccctgcct cccctggcgc aaccgcccga atatcaccag
    12061 ttcgcagacc agcgcacgct gctcggcgtc ccgaacttct tcaacgtttt ctccaacgtc
    12121 gcgtttctgc tggtcggcgg tgccggcgtc gctctgcttc tccgaaaccg tttgccgcat
    12181 gcatcgcgac cgctgctgga ccgttccgtt cgctgggcgt acctggtcct gttcgctgct
    12241 gtagcgctga cttgcatcgg ctcggcttac tatcatcttg cgcccgacaa ctcgcggctg
    12301 acgtgggacc ggctgccgat gagcgtggcg ttcatggcac tgctcgccgc gatgatctcg
    12361 gagagaattc atcccagcgc aggcgtaact ctgctcggcc ccctggttct ggtgggcatc
    12421 gccagcgtgg cgtactggca ttggggcgag gcggccggaa ccggaaacct gcggccttat
    12481 gttgccgttc agacttttgc catcgtcgcc gttctgttga tcgtctttct tttcccgccg
    12541 cgctacagcc gcgggacgga catcctggtt gccgtcgcgt tgtatgcggc tgccaagggc
    12601 gccgagttct acgacgggga gattttctct gccggccggc ttgtcggcgg gcacgcactc
    12661 aagcacctgt tcgccgcgct ggcggtcttc tggattctgc gcatgatctg gaagcgcgct
    12721 cccgtcactg gacccgcggc agcaaacatt tcttcagcga gcgcttcgta agaccgaaat
    12781 ctcgacgcaa agacgaaaag aacgcaatga agaaccataa ggatggaaac caggacagag
    12841 attgacgtgg tagtcatcgc atttacgccg caccccacat gccctgcaat tttccggcgt
    12901 ttatttcatt ctttttgttt ctctttgcgt actttcattt ccgtctgcgt cctctgcgtc
    12961 aacgctctta ttgccggcca tcgcccggtc cagacccggc gaagtgatat ccttcgcggc
    13021 ttcgaatgat cagaagaaga ggccgatgtg aagcacatca aatgggacga gatccccgtc
    13081 gagagatgat cagcattccg gccaacgtgg cacacagcgc tgaagccgtg gagaggctgg
    13141 tcgcctacga catcttgact cctccgcgcc aagactggat caccggcgcg gacgcctatc
    13201 tgcgcagcac ggcggaatag gtaagaaagg aaaacaggtc gcgtgaatcc gcccggatcg
    13261 gcggcaccgt ggcgcgccgc gatatccgac tttcccggcc cttcctgtct tcgtcagcct
    13321 gcccgtgccg ccaactgctg ctcgttcgcg gcatcggtgc cgtacaccgg gggaatggaa
    13381 aaaggccgcc gcccggactc ctgacccatt gcatcgcggt agacaagttc acgcgtgtcg
    13441 aaatcgaact ccgggtgatg gtgttcgtcc catttctcca aaaagtacat gtaaaaggga
    13501 acaaacaggc ctcttgtcgg attgcgatga tggagggcaa acacgtattc cggagtctgc
    13561 ttgatctcac atccgccatg acagtactgc acccacacgt ggtcgcccaa cacgacgact
    13621 ttccagaacg gctggtgatc gtagaatttc agcgtcacct tccttccggt tctgcgaagc
    13681 gcgctcaggt aagaaatgct tgcttcgacc tcttcgcaaa aggagccgaa gtcggaccgt
    13741 tccggcggca tggaatcgac acggagccgc gcgccccggc ttgcgggatt cagcaacatc
    13801 acgcgaattt catatgctgt ctggagcacc gtactgaacc ggctgctctt gcgggcaaag
    13861 gtatcgaagc cggttacggt caatatacag gcgtcccggg tgaccggaag acgtttgaac
    13921 agagccttct cctgccagcg cgtgagccag ttgtaattgc ttttggcgta agccagcgac
    13981 gcaagctttg cattggcaac atagcgacgg ttgtcccacg cggtcttcgc taggttgaac
    14041 agcagcacca gcactgatgc aaacgcaatt tccgtcgtaa agagcaaggt cgagtcagcc
    14101 tcaacccgcg gccaccattg ataaaggatg aactgcgcga cgacgggcag cgtcagggcg
    14161 atgccgatcg cgagcagcgt gataagccca tgcgaaatta tcaggcttaa gcctgataaa
    14221 gcgtgggtgt gtttccgctc atgcattttt tccctccgtt aatcagagtg cgcgtcttgc
    14281 ctcttcgatc catggattcc attgcaagtg acacggcgcg tatcctagcg ttcactcatg
    14341 tacagagtat ttccgaatcg gaaaatttga aatgtgccgg acggattccc gcgcgacact
    14401 gtagtgttgt tggttggaga caggattgcc gaagcagctg cacgacattt acaatgctct
    14461 ccatcggtca ctgtcgccgg cgaacgacgc attctgtgca gccaccgggg agggaaaagt
    14521 aaagactgcg agccgggcga gaacctgaag aagctaccgg gacgtcaacg ttgaagcgct
    14581 gcggatgcgc gattcaaata aaaagccggg atgagacccg gccttttgca ttgcgcggcg
    14641 taaatgatcg cgtcagttct gcgggttcca gttcttcgcg gtttcctttg ccctggcgac
    14701 ctgctccggc gtcattttct tttcgagctt ctcgatcgct tccctggtgt agagcgagtc
    14761 gcgatgggag gtctggggat tcgaggcaga aagcgaaagc cacatgtgcg cggccaccgg
    14821 gtccttttcc acaccctggc ctttctccgc catgatcccg acgtagtatt gcgagcgcgc
    14881 gtcgccttga cgggcggctt tcaggaacca cctcgccgat tcattctggt cgcgcggcac
    14941 gccgaagccg ttgtggtaga acaagcccat gaaatattgc gcttcgggct tgccctggtc
    15001 ggcgagcacc ctgaactcgg caagcgcctt ggcgtaatcg ccggtcccgt aggcctgcac
    15061 cgcgtcgtcg aaaccggcgt gcgccaccag cggcagaacc agggcgagcg aaacaaacag
    15121 atgcttcatc ggtcatcctc gaatgagttg tttggaaatc aagccggaat tgtaacgcgt
    15181 cggcagggcc gaaaacgccg gggatgcgat ccgcgggaac atgaaaagca ggcccgcgct
    15241 cctgcagggc gggaaaaggt gaggcgccag gactgccatc caatttgtgg ccgcgctata
    15301 agggcggctg agctcggaac ggagctctca aggtgggatg actgtgcaga cggggcccct
    15361 aatcttcatc gcgggtccgt actcaacaag gcgatccgta tcgccttctc gcatacacct
    15421 ggcgattgaa acgcagccct ggccaccggt agaaatattc tccctgtcgg agcagactgt
    15481 aaaggggaat ttttcaggaa tcggcgatac tgtaccgtcg atatgggacc gtgcgttggc
    15541 gcgcaggttc cgtgtccagc aaaaatcaag ttcgatttct ctactggagg agcgcgatgc
    15601 ttcagcttcg accgaattgc gagtgctgcg acaaggacct gcctccggat tcgaccgagg
    15661 ccatgatctg ctcgttcgag tgcacgttct gccgcggctg tgcgatcgga gtgctgcagg
    15721 gtgtgtgccc caactgcggc ggcgaactgg tcagacgccc gatccggccg cagggcaagc
    15781 tcgccaggaa tccagcgtcc agcgaacggg tgctgaaacc cggcggctgc cggcacgtag
    15841 cctagcgtcc gccctggggt acctacggcg gctgatcgcc gcctatactg taagttgagg
    15901 gtccggtgcc cggatctcga tcgccgtttt ccgggccggt gatgcaagga tgttccggac
    15961 ccggatccac ggcaatatca agggaccggg tcgcctgcgc caagggagca gatcatgcaa
    16021 accaggctgt tcgcgtcggc ttttccattg gcgccattgg gcgctgccgc aggcggatga
    16081 actaaaaccg cccgccggca ctcgatgtgg ccatgtcagc gatcctaacc cgcatttcgg
    16141 gagttccgta catcgccaag gtcggtgttt tggcggtttg ttactttgcg gcggcaaagg
    16201 cgtccctgct gttcgccata cccccaggat acgccacggc cgtgtggccg ccggcgggta
    16261 tcgcgctcgc cgcgctgctg ctccatggcg tccggatatg gcccggggta tggctgggcg
    16321 ccgtgctggc caatttttcg gtcaatcaat cgctgactct tgcagcagcc atcgccaccg
    16381 gcaactcgtt tgaggcggta tgtgccgcca ttctcgtcaa ccgcctcatc gaatcgccgc
    16441 tcgaattccg ccagccgcat gtggtgttcc gcttcgcgct gatcgccgcg gccagcagtc
    16501 tggtcgcagc cacctccggg gtgggcgctc tgttcgcctc cggaagcatg cccgcccgga
    16561 attttctcga caattggtac acctggtggc agggcgatac cactgcgatt atcgttgtga
    16621 cgcctctgct gctggcatgg ctgcgcgagg cgcgccgcga cgagcccccg cgccgtaccg
    16681 aagtcatcgt gttcggtgga ctgctggtcc ttgccttcgt cgcagtgttc gtttttgcgc
    16741 gctggacccg gccaggaatc gggcatgcac ttccctttct gctgatcccc ttcatggcgt
    16801 gggccggttg ccgcttcgac gaacgtattg tgactgccgc agtattcgcg gtcacgattc
    16861 tggccatttg gggaaccgtc aataacctcg gtccctttgc ctctgccgat ctgaacgaat
    16921 cgctgctatc cctccaggct ttcaccggca ccgtcaccct gatgagcctg gtcctgtgcg
    16981 cccttacccg ggagcgcgcc ggggccgcgg cggcgctgca gcgatcgcat gatcgcctgg
    17041 agctggcggt cagcgagcgc actgccgagt tggcggacaa caatcgccaa ctggcacggg
    17101 acgtcgccga gagggagcgg ctcacccttg tgctgcagcg ccgggaggcg cagcttgcgc
    17161 aggcgcaggc gatcacccat accggatcct ggacctggga cctgagaaca gatcgcgtta
    17221 cctggtccga cgaactgttt cgaatctacg atctgtcgcc ggagcggttc acggaatcgt
    17281 tcgaagctta tctctcgcgc gtgcaccctc aggaccgcga gcgcgtgcag gaggtaattc
    17341 gcaaggcgct ggccgatcat caaccctggg agttgaccga gcggattgtc cggccggacg
    17401 gcgtgatccg ggtgctcagg tccaccggca acgtgctgac cggcgaggat ggcgcggcga
    17461 tcgtcatgca tggcgtatgc gttgacgcca ccgaacagat ccgtctcgag cgcatccagg
    17521 cggttcagaa cggcatcgcc agagcaatgg tgcaggagca ggtcccggcg gatgcgctgg
    17581 tcgcggcact gcgcgttatc tgcgtcacac tcggctggga tgtgggcgca atgtggaaac
    17641 tggatcccga acgcggtgtg ctccaccacg cccattcctg gcatccaata gaaaaagatg
    17701 cgtccgtttt cgttacggca agccggcaga tgctgttcaa gcgtggacat ggacttcccg
    17761 gccgcatctg ggaacaggaa aaagcggtct ggatcgaaga cgtctttgac gactcgaact
    17821 ttccgagagc cggcttcgtc actcaggcgg gtttgcgcgc cgcattcgga ttcccattga
    17881 ttgccggcca ccggatcctg ggagtcatgg agttctttgg cgcgcacgcg cagaaacccg
    17941 atgacgaact gctggaaatg atagccgcgg tcggcaatca actgggcgaa tacatcgttc
    18001 gcaacgaggc acaggtgctg ctcacggaat cggaggaacg cttccgtctg ctggtcgaga
    18061 gggtcaagga ctacgcgatt ttcatgctcg atcccgaagg gcgtatcgca agctggaatt
    18121 cgggagcaca gcgcatcaag ggctaccgga ccgacgagat cgttggcaag catttttcct
    18181 gtttctaccc gccggaagac attgcaagtg gccaacccga gcgagcgctc gagatcgccc
    18241 gggcggcggg ccgctatgaa gcggaagcct ggcgcgttcg caacgatgga tcccggttcc
    18301 tggccaacgt cgttatcacg ccgctacgcg ccgacgacgg cgggctgcgc ggctacgccg
    18361 aggtcacccg cgacatcacg cagagccggc gttcggaaga tgcgatgagg cacagcgaga
    18421 cgatgttgcg cagcgtgctc gacacgcttc cggtgggcgt ttggatgatc gattgcaaag
    18481 gcggcattgt cgaggccaac ccggcggcac agagaatctg ggctggctcg cgctatgtcg
    18541 gcatggacga gtacggcgtt tacaagggct ggtgggccgg aaccgaccgg ctgatccagc
    18601 cgcacgagtg ggcgggggcg agggcggtcg agcgtgggga gacctcgatt aatgaactgg
    18661 tggacatcga atgtttcgac ggcacgcgca agaccatcgt gaattctgca gtgcctctgc
    18721 gggatgccga gggccggatc gcgggcgcca tcatcgtaaa cgaagatgtc accgcccgca
    18781 ggcaggcgga gcaagcgctg accggcgtcg cggcgggcgt ttccgcggca acgggcgcgg
    18841 agttcttccg cttgctcgtc gaacaagtga cccaggcgct cggcgcggac ggagcgatca
    18901 tcggtgaact gaccggaaag aaagaaggcg aggtgcgcgt cgtcgctggt ctcatggacg
    18961 gcaggatggt cgatagtttc acttacgact gccacgggag tccgtgtgcg atcgtgctca
    19021 ccgaaaatgc tgtcgttcat gcagacgatg cgcgcgcgcg cttcagccac acgccggtcc
    19081 tcgaccggta ctccgccgac agctatttcg gcatcggcct ctggggagcc gacgggggta
    19141 cgctcggggt catgatggtg atcaatcgca agcccttgcg caatccggaa ctcgcgaaat
    19201 ccatcctgag aatcgtcgcc tcgcgcgcgg cagcggaact ggagcgcaag cgcaacgaag
    19261 aaaccatccg ctcgcttgcc atccgcttgc aggaaatgat cgaggaggaa cgcacccgca
    19321 tcgcccgcga gatccatgac gaactggggc aggccctgac cgggctgaaa atggatctgt
    19381 cctggctatc gaagtcggtc gataagcgtg actcgaaggt gatggcgcag atcaagtcca
    19441 tggcggaatt catcgactcg ctgattcaca gcgtgcggcg catatccacc gatctgcgcc
    19501 ccggagaact cgacgacctg ggcctggtag cggcgatcga gtcgcaggcg caggatttcg
    19561 aaagccgcac cgggataaaa tgccggttga gcctgccgga agcggatctg gcggtagacc
    19621 ccgggatcgc aaccgcggta ttcaggatct gtcaggagtt gctgaccaat gtcgcgcgtc
    19681 attccggcgc caccagggtt agcctggacc ttgagtgcaa cgacgcctgg ttgagtcttg
    19741 cggtgcagga tgatggccgc ggcattcgcg tcgccgaggc ctccagccga aacgccatcg
    19801 ggctggcggg gatccgagag cgcgcaaaaa tgttcggcgg cagcgtgact atcaagggaa
    19861 aacccggaaa gggtacgctg gcgcgggtac gcattccgat tggccgcgcc cgggacgccg
    19921 cgaacgggaa cgacgatgtg gctgcagcct agatttcgag tgtaccgggc caccaactcc
    19981 cttatttcgc accacggacc gcgatgaaag ttctgattgc agatgaccac gcggtggtcc
    20041 ggcgcgggct caggcagatt ctggaggccg agccggatct ggaggtggcc ggcgaggcaa
    20101 cctccggcac cgaggtcatg gaggcattac gcatgcaacg ctacgatgtg gcagtactcg
    20161 acatcaccat gccgggtcgc ggcggactgg acgttcttca cgatatccgg cagaacttcc
    20221 ccgagctgcg cgtcctgatt ctcagcatgc acccggagga gcgctacgcg ttgagggtgc
    20281 tcaaggccgg cgcggccggc tacctcacca aggagagcgc cgccgacgaa ttgatcacag
    20341 cgctgcgcaa ggtggcggcc ggaggcagat atgtcagccc gacactggcg gaacgcctgg
    20401 cggacgaaat tggcatcgga atcgaccaac ccctgcacga tcggttgtcg gatcgcgagc
    20461 accagatcct gtgcatgatc gccaatggca tgaccgtcag cggcatagct tcggaattgc
    20521 acctgggtac aaacaccgtg agtacctacc gggcgaggtt gttgggaaaa ctcaaactgg
    20581 ccaataacgc cgagatcacg cactacgcta tccgcaaccg gctggtggac tgacaccggc
    20641 tcagcgacgg gcgcggccca agttcccgtt tgcaacgctt gccctgcctg tacgcgcggt
    20701 tcatgtagat caaaccatga catccgtacg ggctgccgta ctacagaaaa atcactgttt
    20761 cgacgatttc ccccgtggct ggccggggct attctgcaaa ctgtacggtc tgcggaccca
    20821 tgcctgccgg acttcaaagc atgaaagtgg tgattgtcga cgattcagcg ctgcttcgac
    20881 agcgtctggc agccatgatc tcccggctgt ccggggtcga aatcgccggt gaagcaaaga
    20941 gtgtggcgca ggcgctggag ctgatcaggc gctgccatcc ggaagcggtg atgctcgata
    21001 ttcgcttgaa ggacggcaac ggattcgacg tgttgcggca ggtcaagcgc gagcccggcg
    21061 cgccggtagt catcatgctg accagctttc cgagcacgaa ggcacggcag acctgccagg
    21121 cgctgggcgg tgattttttc ttcgacaagg ccacggagtt cgagagggcg attgaggtgc
    21181 tgaccgacct ggagttcgcg cggcgcacat cgaaatcgtc cgaattcaac agacctgccg
    21241 gagccgggtg ctgatcgacg agaaattcgc caacagaggc tcgttataga aagcggacat
    21301 aatttaggga gaacggtgtg tcatggcgga agaactataa agtgggttct gccgtcagta
    21361 agcgcagctt ttccggcgag atggcgggtg tcattcccct ctctggcttt cagcaactga
    21421 ctcagagcgg ccaccgactg gttcgcgttc ctcccctcgc gacagccgga ttctgcagga
    21481 cgcacttacc gacgtcagtt ctgccagcga accgccatct cgttttttct tccccagtcg
    21541 cagcggacct cccggccgct gcagaccggc ccggtgcgac gaggcgccgg gccggcgaca
    21601 cgcgggtccg caaactccaa ttcgattcgc cactggttat tctcggggag caaaaagagg
    21661 caaaagaaca agtctccctc cgactcactt gagtaaggtc ggtaaatggc agcgttgtcg
    21721 cggatagagc cggttccgcc ggcgaccgca ctaacctgtt actcccgttc cgccttcccg
    21781 gctgaaatat gaagaaggaa tttcgcaggc ttgcgtccga ttcgaaccgc ggaactggcg
    21841 atgccgcggc tgacgccttt cccacaggct cggggaaaga gatcaggata ctttttgtcg
    21901 aggacgtgat ctccgacttc gaacttacca tccgggaact gaagcaagcc ggggtcgcgt
    21961 gcgcagttca tcgagtggac actcgcgaag gccttcttcg ggagttgaaa gacttcgagc
    22021 ccgacgtgat tctctccgat ttctccctgc cgaccttcga tggcttgtca gttctgatga
    22081 tcgtccgcga gctgcgtccc gacataccat gcatattcgt ttccggcacg ataagcgaag
    22141 accagctcat atcgctggtc aggcatggcg cagccgatta tgtgctgaag agcaacctgg
    22201 aacggattcc gcccgccgtt cttcgcgctg ttcaggaacg caatgaacgc gcggcccggc
    22261 ggcaggctga gcaagcactc aaagagagcg aggcacttct cgactcagtc ttcgacaaca
    22321 tacccagcat gctgttcatc aaggatgcaa aggatctgcg atttgtccgc ttcaatcgca
    22381 ccggcgagga gttgctcgga cgttcggaga aagacctgct tggaaagaac gactacgact
    22441 tttttcccaa ggagcaggcc gatttattcg tcgccaagga tcgggagacc ctggcaagca
    22501 accagcgggt cttcattatg gaagagaaga tcgaggtcaa agacggaacc gagaggatgc
    22561 ttcagaccaa gaagctggcc atactggacg agcagggtaa tccgcgtttt cttcttggca
    22621 tttccgagga cgtcaccgag cgcaaacaag ccgagcagca aattcgggac agcgaggcca
    22681 agtaccgcgg actcatcgaa caagcctcgg acggcatatt cgtcagcgac gcggaaggca
    22741 atctcgtcct cgcgaattcc cgtctgtgcg agcttctggg ttacgccaaa gacgaggtga
    22801 cgggactgaa cgcgaggcag acttatctcg aagaagaaag ggagacccat gccaagctcc
    22861 tggaacaggt tcgtgcggga cagactcttc gttatgagcg aactgtgcaa cgcaaggatg
    22921 gaagccattt ccctgccgaa atcagtctca agatgctcga taacggactc atccagggaa
    22981 tctttcacga catcaccact cgccgtgcgc aggagcacaa gatcgcgcgg ctcagccgga
    23041 tccaggccgt attgagcggc atcaactccg cgatcgtgcg cattcgggat cgccagacat
    23101 tattcgacga ggtctgccgg ctgtccatcg agcatggcgg cttcgccctg gcgtggatca
    23161 ggatgacgac tccagacgcg cagggtggga ggccgattgc gtggcgttgc tcgaacgaca
    23221 gctatctgga tctcattcct ctttccatcg agaccgatgc cggccgcgcc cacgcggagc
    23281 gcgtcatgtc ggaaaagagg gcggtggtct ataacgatct cacgcaagcc ggcgatttcc
    23341 ccttcgccgg acaggctttg caggctggcg ccctgtccct ggcaaagctt cccctggtgg
    23401 tatcgggtga agccgttgga a
//
LOCUS       DASQOP010000102         4290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1049621, whole genome shotgun sequence.
ACCESSION   DASQOP010000102 DASQOP010000000
VERSION     DASQOP010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049621"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            537..1532
                     /locus_tag="VE030_04185"
     CDS             537..1532
                     /locus_tag="VE030_04185"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HZF22650.1"
                     /translation="MIRASFNASPRYFLLLGLLSCTLGAPITRAATDAATVEVGRRLD
                     RCLNALDTGAEPLPPYRRLIVEEVCPGLAQAVSELPAAESLSQPLDLQTTPNQLRDLR
                     ALLGSIRSPPAGLERFDFAALPELLARTLQVEPKPPVSWWQRLKDWLAQKLRGSGEPD
                     YRWLTEFLKSLDPPEWLADLILRTSVAVILLLALAIVVNELRAANLSSWLQRRSRTQR
                     ASRVPATAGASRLAWKDVTNLPPGQQPAALLRLVLQELIERGLLPDDQSLTNREMLAQ
                     LGAAARTHATSFAELAAAADAVLFGNREVVAAQLAPLHQAARAIVGTPAVGASPR"
     gene            1529..2797
                     /locus_tag="VE030_04190"
     CDS             1529..2797
                     /locus_tag="VE030_04190"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HZF22651.1"
                     /translation="MRERLVTLLGALAAVYLVFVLLVGPREPNAAPESMPTTADRGSR
                     GLQGLQSWIESSGIRVKSLRSRYDQVPTDAALPERGNLMILTLPQSLPVRQLEIDALK
                     DWVERGNSVLVLDGSIEVFAQPQARRRQPSSMLAALGFSLEFADAELDEADKSGDAAA
                     AKLELDKDGRPQPRGLTIRGHHPVLQGVQVVQVRTHSLYPLSLTPKEKFRTALVLLED
                     PVTRSPAFWQVRIGDGTGWISGYADLFGNVALGASDNARLLANVVASSLGPDGWVVFD
                     DMHQGLTEVYDPNAFFGDARLHATLWFIVGFWLVYVAGRSNRIAPLVQRAELPRAVDF
                     VRAVGGLFGRRLAGHEVALGLLRHFFAELRGVRAGADGIDWDLLARFPRVPRSQIDEL
                     RAMHSGLAQGRTPDLVRLNNLINNIRKLQQ"
     gene            2794..3744
                     /locus_tag="VE030_04195"
     CDS             2794..3744
                     /locus_tag="VE030_04195"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZF22652.1"
                     /translation="MSNPASSLEQQLRTQLDNVVFGMDNVIHGLCIGLIAGGHVLLQG
                     VPGIGKTLLAKSVAAALGGTFNRVQCTADLMPSDITGIHIFSREEKKFEFVPGPLFAN
                     IVLVDEINRSGPKTQSALLQAMEEFQVTIDRKTYSLPQDFLVIATQNPRDFEGTFPLP
                     ESQLDRFLMRLDVDYPAAEMEHKVLRTYDLRGGHFNATAPIAPLAGDALALARTQVAK
                     VHVSEAIYGYVSAIATASRNHPQVNLGLSTRGALALMRCARIAAALRDAEFVAPDDVK
                     AMAGFVLPHRLMLTPDAALEGIGDLQVAQSILDSVKVPRQ"
     gene            4091..>4290
                     /locus_tag="VE030_04200"
     CDS             4091..>4290
                     /locus_tag="VE030_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22653.1"
                     /translation="MNLTALSHIVAAAVTLLGIVGQWQDDPWPDLWRVPAASLLIGLL
                     LEGVAARRRGIRISPRIPERVML"
BASE COUNT          741 a         1420 c         1364 g          765 t
ORIGIN      
        1 ctcggtctcg gcgacgacgc cgacgtgaac accatggcca gtgccgtggg aatgatctgg
       61 cgggcgctgg taatgtggat gctgatcatt gcgctgatcg ccatcgcccg ctgggtcggc
      121 ggctagggaa gctctgcata agtcccacga cgcgcgttgc gccaggtgtg gatgactgct
      181 agggacgccg gagcgcggaa cagggggccc ctctggggat gtgaccgcgc aggcgtccca
      241 acgagcgcac atgagcggcg tgcaatgcga gcgactccgg gtcgcatccc ccggccaagg
      301 ccggcggaac ctgctccgcc tgcgcgctcg cgctccgcag atgcggctga agccccagca
      361 aggagcgtaa caacgcaggc gccgcacctg gcacaaccca atgacatttc gactctttcg
      421 agggaccggg cgattcgggc cgatgcctgc gtcggctcct ccttatgtag gccggctaca
      481 cggcgtcctc gccgacttgc cctcgacccg aatcgcctcg gtcgcgcatg tggaacttga
      541 tcagagcttc ctttaacgcg tcgccccgct atttcctact gctcggcctg ttgtcctgca
      601 cgctgggagc gccgatcacg agggctgcca ccgacgccgc caccgtcgaa gttggccggc
      661 gcctggaccg gtgcctcaac gcgctcgaca ccggggccga gccgctgccg ccttaccgac
      721 gcctgatcgt ggaagaagtc tgcccggggc ttgcacaggc ggtttcagaa ctgccggcag
      781 cggagtcgtt gagccagccc ctggatttgc aaaccacccc taaccagctc cgtgatctgc
      841 gcgcgctgct gggctctatt cgaagcccgc ccgccggcct cgagcgtttc gattttgcgg
      901 cgctgccaga actgctggcg cgcacgctgc aagtcgaacc gaagccgccg gtttcctggt
      961 ggcaacgact caaggactgg ctggcgcaaa aactgcgcgg cagcggcgaa ccggactacc
     1021 gctggctgac cgaatttctg aagtcgctgg atcccccgga gtggcttgca gacctcatcc
     1081 tgcgcaccag cgtggccgtg attttgctgc tggcgctggc gatcgtggtc aacgaactgc
     1141 gcgcggccaa tctgtcgtcg tggctccagc gacgaagccg cacgcaacgc gccagccgcg
     1201 tgccggccac cgccggcgcg tccagactgg catggaagga cgtgacgaat ctcccccccg
     1261 gccagcaacc ggcggcgttg ctgcgcctgg tgctgcagga actgatcgaa cgcggcctgc
     1321 tgcccgacga ccagagcctt accaacaggg aaatgctggc acagctcggc gcagcggcac
     1381 gtacgcacgc cacatctttt gccgagctgg cggccgcggc cgacgcggtg ttgttcggca
     1441 accgcgaggt ggttgcggcg cagcttgcgc cgctgcatca ggcggcacgg gcaatcgtcg
     1501 gcactcccgc tgtcggcgcc agtccccgat gagagaacgc ctggtcaccc tgctcggcgc
     1561 gctggctgcc gtgtatctgg tgttcgtgct gctggtcggg ccacgcgaac ccaacgccgc
     1621 gccggagtcc atgccgacca cagcggaccg cggcagccgt gggctgcagg gtctgcaatc
     1681 ctggatcgag tcgagcggca tacgcgtcaa gagcctacgc agccgctacg accaagtgcc
     1741 gaccgatgcg gcactaccgg agcgcggcaa tctcatgatt ctgacactgc cgcagtccct
     1801 acctgtacgg caattggaaa tcgacgcgtt gaaagactgg gtcgagcgcg gcaactctgt
     1861 gctggtgctc gacggaagca tcgaggtttt tgctcagccg caggctcgcc gccggcaacc
     1921 gtcgagcatg ctggccgcgc tcggattcag cctggaattc gcagacgcgg aactcgatga
     1981 ggcggacaag tccggggacg ccgccgcggc gaagttggaa ctcgacaagg atggcaggcc
     2041 tcagccacgc ggattgacca ttcgaggcca ccacccggtc ctgcagggag tgcaagtggt
     2101 gcaggtacgc acgcattcgc tctacccgct gtcgctcacg ccgaaggaaa aattccgcac
     2161 cgccctggtt ttgctcgaag atcctgtgac gcgatcgccg gcattctggc aggtgcgcat
     2221 cggcgatggc accggctgga tcagcggtta cgccgacctg ttcggcaatg tcgcgctcgg
     2281 cgctagcgac aacgcccgtc tgttggccaa cgtggtggcc agttcgcttg gcccggacgg
     2341 ctgggtggtt ttcgacgaca tgcaccaggg cctgaccgaa gtctacgacc cgaacgcctt
     2401 ctttggcgac gcgcgtctgc atgccacgct gtggttcatc gtcggcttct ggctggtcta
     2461 tgtggcaggg cgcagcaacc gcatcgcccc gctggttcag cgagccgagc tcccgcgtgc
     2521 cgtggacttc gttcgtgcgg tgggcgggct gtttggcagg cgtttggccg gccacgaagt
     2581 tgcgctgggg ctgttgcgcc actttttcgc cgagttgcgc ggcgtccgtg ccggcgcaga
     2641 cggcatcgac tgggacctgc tggcccgctt tccgcgcgta ccgcgcagcc agatcgacga
     2701 attgcgggca atgcattccg gtctggcgca gggacgcacg cccgaccttg tgcgcctgaa
     2761 caacctcatc aacaacataa ggaaactcca gcaatgagca atcctgcctc cagcctggaa
     2821 cagcaactgc gcacgcaact ggataacgtg gtgttcggca tggataacgt gatccacggg
     2881 ttgtgcatcg ggctgatcgc cggcggtcac gtgctgctgc agggcgtgcc cggcatcggc
     2941 aagactctgc tggcgaaatc ggtcgctgcg gcgctcgggg gtacgttcaa ccgcgtgcaa
     3001 tgcaccgccg acctgatgcc ctccgacatc accggcatcc acatcttcag ccgcgaggag
     3061 aagaagttcg agttcgttcc cggaccgctg ttcgccaaca tcgtgctggt cgacgagatc
     3121 aaccgctcgg ggccgaagac ccagtcggcg ctgcttcagg caatggaaga attccaggtc
     3181 accatcgacc gcaagaccta cagcctgccg caggacttcc tggtcatcgc cacccagaac
     3241 ccgcgcgatt tcgagggcac ttttccgctc cccgagtcgc aactcgatcg cttcctgatg
     3301 cggctggacg tggactatcc ggcggcggag atggagcaca aggtgctgcg cacctacgac
     3361 cttcgcggcg ggcacttcaa cgcgactgcg ccgatcgccc cgcttgcggg cgacgccctc
     3421 gcactggcgc gcacgcaggt ggcgaaagtg cacgtgtccg aggcaatcta cggttacgtc
     3481 agcgccatcg caaccgccag ccgcaaccac ccgcaagtca acttgggcct gtccacccgc
     3541 ggcgcgctgg cgctgatgcg ttgcgcccgc attgccgcgg cgctgcgaga cgccgaattc
     3601 gtcgcgccgg acgatgtcaa ggccatggcc ggtttcgtcc tgcctcaccg cttgatgctc
     3661 actccggacg cggcactcga aggcatcggc gaccttcagg tcgcacaaag catcctcgac
     3721 agcgtcaagg taccgcgcca gtgagacgcc gcgctatgtc gttacggctc agctttccag
     3781 gtttttttct ggacattcgt gtgggtgatc ggcgcccgga tgttcaagaa ttgatcgcct
     3841 tgcggaaaca ttcgtcgcca aacctttacg gccaagcgtt aaccgccagg gacgccaggg
     3901 gaagtcaaat gcaagaatca agtgaatgtg caaacccagt gcaagtatct gcgttgcggc
     3961 cggcccgtta cctaatctca gcctcgcctt cacgctattg tctttccttg gcgtccctgg
     4021 cgtccctggc ggtggatgcg ttattggtga ggcgttcgaa acccacaaag aagtctaaag
     4081 tacctccaag atgaacctga ccgcactttc ccacatcgtc gccgcggcag tgacgctgct
     4141 cggaatcgtc ggccagtggc aggacgatcc ctggccggac ttgtggcgcg tgccggcggc
     4201 gagcctgctg atcggactgt tgctggaagg cgtggccgca cggcggcgcg gaatcagaat
     4261 ctcgccccgc atcccggagc gcgtcatgct
//
LOCUS       DASQOP010000103        15270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2657738, whole genome shotgun sequence.
ACCESSION   DASQOP010000103 DASQOP010000000
VERSION     DASQOP010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 15270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15270
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2657738"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            394..1080
                     /locus_tag="VE030_04205"
     CDS             394..1080
                     /locus_tag="VE030_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HZF22654.1"
                     /translation="MQPEIQYGAQSGQLTLAQNKVLRNTYMLLALTMVPTVIGALVGM
                     ATGGIIMQHPIMMSLVMIAGVIGLQFAIAANRNSGLGVVLLLGMTFILGWWLGPLLNI
                     ALSFRNGPQLIGMAAAGTGGILLAMSAIATTTKKDFSFMGKFLFVGMIVLLLAMIANM
                     FFQIPALALALSALVVVVFSLFLLHDVSRIVNGGETNYIMAATGVYMSLFNIFVNLLQ
                     LLMAFSGERD"
     gene            complement(1145..2458)
                     /gene="rlmD"
                     /locus_tag="VE030_04210"
     CDS             complement(1145..2458)
                     /gene="rlmD"
                     /locus_tag="VE030_04210"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HZF22655.1"
                     /translation="MKRDSVGPAVLVESLDQEGRGVAHVDGKVIFIEGALPGEVVEFA
                     SYRRKPNYEFAEARRIVRSSASRVVPRCRHFGVCGGCSMQHLEPRAQVAAKQRVLEEN
                     LARIGKVTAETMLSPIHGPTWEYRYRARFSARYVAKKGALVGFRERHSSFVADMQTCE
                     VVPARISVLLVPLRQLIASLSIRERLPQVELAIGDAVDVLVLRVLEPPDVSDQDELRQ
                     FAAEHRVQLFLQPKGPDSMYPFFPLDAPQLQYRIPDFDLTFPFNPTDFTQVNPEINRA
                     LVRRAITLLDPQPGDRIGDFFCGLGNFSLAIARSGAQVTGIDGNPGLLQRARENAVRN
                     GLEARCRFVERDLFAITGEDWSGLAPFDRVLIDPPRDGAIELVKAIGKSPPARIVYVS
                     CNPATLARDAEVLVHSHGYRLAAAGVVNMFPHTSHVESVALFAMP"
     gene            complement(2553..3329)
                     /locus_tag="VE030_04215"
     CDS             complement(2553..3329)
                     /locus_tag="VE030_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014896903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HZF22656.1"
                     /translation="MTPVLAFDIETIPDIEGLRRLYGHGPQTSPAEVAQAAFDRRREA
                     TGSDFLPLHLHRVVAISCALRDRDTFRVWSLGEAGEPEHDLVQRFFDGIEKYTPQLVS
                     WNGSGFDLPVLNYRALIHGVRAARFWDMGEDDRDFKWNNYISRYHSRHLDLMDVLAMY
                     QGRGNAPLDQIAQLLGLPGKLGMDGSKVWDAYQAGSIEAIRHYCETDVANTYLVYQRF
                     QLIRGALDETQYRRECDLVRSTFSKSTDAHWREFLDGWQA"
     gene            3642..5471
                     /locus_tag="VE030_04220"
     CDS             3642..5471
                     /locus_tag="VE030_04220"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012735170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HZF22657.1"
                     /translation="MDCPDYVRNRQLREWVGEIAQLTRPDRIHWCDGSQAEYDALCHQ
                     LVAGGTFRKLHSKKRPDSYLALSDPSDVARVEDRTFICTTRSEDAGPTNNWTDPQEMK
                     QRLLALFDGSMRGRTLYIVPFSMGPLGSPISHIGVELTDSAYVAISMRIMTRIGREAI
                     EALGTEGGFVACLHSVGMPLGPGQEDVRWPCNKDHKYIVHFPEEKAIWSYGSGYGGNA
                     LLGKKCFALRIASCMGRQQGWLAEHMLILGVESPQGEKNYVAAAFPSACGKTNFAMMI
                     PPAAFKGWKVTTVGDDIAWIKPGADGRLYAINPEYGFFGVAPGTSEKSNPNAMATLSR
                     NCIFTNVAITPEGDVWWEGMTEEPPAQLTDWQGLPWTPDCGRKAAHPNARFTVPASQC
                     PSMDAAWETPEGVPISAFVFGGRRGTTVPLVTESFDWTDGVYMAATLGSETTAAATAK
                     VGEVRRDPMAMLPFCGYHIGDYFDHWLGIGRGLKHPPRIFHVNWFRKDGDGKFLWPGY
                     GENMRVLKWIVDRCQGRLEAAESPLGWMPRRQDMVWDGLKVSNETFDELMSLDREAWT
                     EELRLHEQLFAQIQDRLPRELAQKRELFLARLSGAPARWTVPF"
     gene            complement(5650..6561)
                     /gene="cysM"
                     /locus_tag="VE030_04225"
     CDS             complement(5650..6561)
                     /gene="cysM"
                     /locus_tag="VE030_04225"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HZF22658.1"
                     /translation="MDRNPVSAYQTLDDFVGNTPLVRLKRIPGRTSNVVLAKLEGNNP
                     AGSVKDRPALSMIRRAEERGQIKPGDTLIEPTSGNTGIALAMVAAMRGYRMVLVMPEH
                     LSVERRQTMAAFGARFVLTPKEGGMETARDIAEQMQARGEGLILDQFANPDNPLSHYE
                     GTGPEIWRDTRGQITHFVSSMGTTGTIMGCSRFFKEKDPAIQIFGCQPTEGSQIPGIR
                     KWPEAYLPKIYDRTRVDRVIEVSQADSEEMTRRLAKEEGIFAGISSGGALWAALQVSR
                     EVENATIVTIVCDRGDRYLSTGVFPAE"
     gene            complement(6625..7623)
                     /gene="rfaD"
                     /locus_tag="VE030_04230"
     CDS             complement(6625..7623)
                     /gene="rfaD"
                     /locus_tag="VE030_04230"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HZF22659.1"
                     /translation="MYIVVTGAAGFVGSNVVKALNARGETGILAVDNLTRADKFRNLA
                     DCEIADYLDKEHFRAALDAGSFDGEIAAILHQGACSDTMQTDGRYMMENNYRYSVDLL
                     AYCQSESVPLIYASSASVYGSGAVFKEGRENEKPLNVYGFSKFLFDQLVRRRWEDNTA
                     QVAGLRYFNVYGPRESHKERMASVAFHCFNQFRETGKVKLFEGSGGYAAGEQRRDFIS
                     IEDVVKVNLYFLDNPRKTGIFNCGTGASQTFNDIAVATINALRRAEGKNPHSLAELRS
                     QGMIEYVSFPAGLREKYQSYTQADLAQLRGCGYAAPFFDVETGVTRYVEWLLDAAP"
     gene            complement(7753..8706)
                     /gene="rfaE1"
                     /locus_tag="VE030_04235"
     CDS             complement(7753..8706)
                     /gene="rfaE1"
                     /locus_tag="VE030_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HZF22660.1"
                     /translation="MSSSSFIPHPSSLRSARVLVVGDVMLDRYWFGEVSRISPEAPVP
                     VVKVGRTEERLGGAANVARNIVAVGAQASLLSVVGQDEAGDCLQRLLESVGITAQLHR
                     DPGLATTIKLRVIGRQQQLLRIDFETEPGHEVLMSKLADFESRLPKADLVILSDYGKG
                     GLRHIEKMIDLARGSGKPVLVDPKGDDYGRYRGATLVTPNRAEFREVAGRWKSEDDLT
                     ERAQIMRRELGLDALLVTRSEEGMTLYRDQQRLHVPAVAREVYDVSGAGDTVIAVLGV
                     MLAAGAGFAEAVQFANKAAGIVVGKLGTAVVHPEELFEEQG"
     gene            complement(8825..10147)
                     /locus_tag="VE030_04240"
     CDS             complement(8825..10147)
                     /locus_tag="VE030_04240"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012213840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HZF22661.1"
                     /translation="MKITIIGSGYVGLVSGACFADLGNDVVCLDLDEHKVAALNSGGV
                     PIYEPGLEQLVRHNAAAGRLRFTTDVEESVAHGSLQFISVGTPADQDGSADLRYVLGA
                     ARSIGRHMDDYRVVVNKSTVPVGTAERVRAVIEEELKTRRVTLPFSVVSNPEFLKEGA
                     AVNDFMRPDRIVVGSSDERATQIMRQLYAPILRNHDRLLVMDPRSAELAKYAANAMLA
                     TRISFMNELANLAEKLGANIEAVRHGIGSDQRIGYHFLYAGVGYGGSCFPKDIQALQH
                     TAGEYGMQMTIVDAVEKVNRTQKQRILEKVRMRFGEHLDGRRFAVWGLSFKPNTDDMR
                     EAPSRVVINGLLARGASVNVYDPVALKEAKLVFAKEPAVHLAPSTTAALDGADALIIV
                     TEWKEFRSPDFDDMKRRLKAPIVFDGRNLYDPVLMREAGFEYFSVGRP"
     gene            complement(10144..10857)
                     /gene="pyrF"
                     /locus_tag="VE030_04245"
     CDS             complement(10144..10857)
                     /gene="pyrF"
                     /locus_tag="VE030_04245"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HZF22662.1"
                     /translation="MTTGSRIIVALDYADARSATALVDRLRPSVCRLKVGKELFTAAG
                     PEFVAMLVKRGFDIFLDLKFHDIPNTVAQSCKTAAGLGVWMLNVHALGGRAMMQAARD
                     AIGTAPGRPRLIAVTVLTSMAETDLHEVGIDATPLEEALRLAALAKRCGLDGVVCAAF
                     EAGAIRAHLGEAFLRVTPGIRLPEDSAEDQKRVMTPRQALESGSSYLVIGRPVTRAAQ
                     PLEVLARINAEVVLLENGK"
     gene            complement(10854..12029)
                     /gene="lapB"
                     /locus_tag="VE030_04250"
     CDS             complement(10854..12029)
                     /gene="lapB"
                     /locus_tag="VE030_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HZF22663.1"
                     /translation="MEFELWWLLGFPLFFALGWLAARVDIKQLLSESRALPLSYFKGL
                     NFLLNEQPDKAIEAFIEVVKVDPQTIELHFALGSLFRRRGEVERAIRMHENLLDRPDL
                     APDQKLTALYELAQDYLKAGLLDRAEEMFGKLQGTQYEQQGLKFLLEIFEQEKDWKKA
                     IDASRRMDAVAGQSSAVDIANFYCELAVGEMTHSRWAAARDYVREALNVNRKCARASI
                     LLGDIELTQHNNERAIEAWKRIEGQNPAYLSLIADKLLLAYRNLGRQDEGLQLLRGYL
                     GSYPSLDLLNATFQGEIEHRGADSAYRLVRDELARNPTLLGLDKLLEAQLLEVPAERR
                     QDLQLIKNLVHQHTRSLALYRCDNCGFKARQFYWHCPACNGWETYSPRRTEERELDS"
     gene            complement(12033..12302)
                     /locus_tag="VE030_04255"
     CDS             complement(12033..12302)
                     /locus_tag="VE030_04255"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HZF22664.1"
                     /translation="MRYISWALRILLFVLLFGFALKNTDPVVVRFYLGSQWEAPLAMV
                     VLVFFGVGALAGVIACLTYFYRQRREILQLRKELRAKATAPEDAR"
     gene            complement(12406..12693)
                     /locus_tag="VE030_04260"
     CDS             complement(12406..12693)
                     /locus_tag="VE030_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HZF22665.1"
                     /translation="MTKSELIAKLAAHFPQLVASDAELAVKMILDAMAKSLAQGHRIE
                     IRGFGSFGLNYRPPRVGRNPKSGDKVHVPAKYVPHFKAGKELRERVDFKSQ"
     gene            complement(12702..14426)
                     /gene="rpsA"
                     /locus_tag="VE030_04265"
     CDS             complement(12702..14426)
                     /gene="rpsA"
                     /locus_tag="VE030_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HZF22666.1"
                     /translation="MTTETLIPDAAASENNFAAMFEESLSRQEMRAGEVITAEVVRVD
                     PNFVVVNAGLKSESFISADEFKNDRGELEVKPGDYVTVAIEALEDGYGETRLSREKAK
                     RLAAWHDLEKAMEQGTLVQGLVNGKVKGGLTVMVNGIRAFLPGSLVDIRPVKDTNPYE
                     GKNLEFKVIKLDRKRNNVVVSRRAVLEASQGAEREALLASLQEGATVKGIVKNITDYG
                     AFVDLGGIDGLLHITDLAWRRVKHPSEVLNVGDEVTAKILKFDQEKNRVSLGLKQLGE
                     DPWVGLSRRYPPGTRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSRVV
                     QLGDEVEVMILEIDEDRRRISLGMKQCKPNPWEEFSANYKKGDKVRGQIKSITDFGIF
                     IGLPGGIDGLVHLSDLSWSVPGEEAVRNYKKGDEVEAMVISIDVERERISLGIKQLEG
                     DPFTNFVATHDKGMVVDGVVKTIDPKGAVVQLDTDVEGYLRASEIARDRVEDIRTHLK
                     EGDKVNAMIINIDRKNRNINLSIKAKDAADESEAMQKHAAESASNAGTTNLGALLKAK
                     LDNKNSAQ"
     gene            complement(14548..>15270)
                     /gene="cmk"
                     /locus_tag="VE030_04270"
     CDS             complement(14548..>15270)
                     /gene="cmk"
                     /locus_tag="VE030_04270"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HZF22667.1"
                     /translation="KTFPDFFDRFAEIAGGATVPVIAIDGPSASGKGTVARRVAQRLG
                     FHYLDSGALYRVVALAAMRQGIAMDDAGALGRLAQSVPVEFHGDNIRLDGDDVTDAIR
                     TEEVSAAASHVAAIHEVRQGLIKRQRAFRRPPGLVADGRDMGTVVFPDAILKVFLTAS
                     AEERALRRYKQLIDKGFDANLATLLQEISQRDARDSLRSVAPLQKSADAQVLDTTGLS
                     IEDAVAQIVQWFESAQRRAPSR"
BASE COUNT         2901 a         4793 c         4565 g         3011 t
ORIGIN      
        1 aaatcttttc agcgaagcaa aatcggttgg aggatcaaat actaagcggg catttgccgc
       61 ccttttgggc tttatttcaa gaccggtgca tttaaccgct ctgccaccct tccgaatttc
      121 tgccggtttt agctccgaca taacttgaag gttagtctgc gctagcgccg gcttcgcagg
      181 cgccaagacc agtgcagtag ttccggcata acctgaaaac caggttagcc tccactgggc
      241 gaagcttcgc gtcgcccaac cgctctgcca acctccgaag ggcgcaaatg atacgcgcat
      301 agcccgcggc cgccaaatga ggcgttgaaa ctttgatccc ggttaagtag tcttacgctc
      361 tgtaatttcc cacactgagg agtatgtaac catatgcaac cagaaatcca gtacggtgcg
      421 caatcggggc agttgacgct tgcgcagaac aaggtgttgc gaaacaccta catgctgctt
      481 gcactgacca tggttccaac cgtcatcgga gccttggtcg gcatggccac cggcgggatc
      541 atcatgcaac acccgatcat gatgtcgctg gtgatgatcg cgggtgtcat aggcttgcaa
      601 tttgccatcg ccgccaatcg caacagcggc ctcggcgtgg ttctgctgct cgggatgacg
      661 ttcatcctcg gctggtggct cggacccttg ctcaacatcg ccttgtcgtt caggaatggc
      721 ccgcaactga tcggcatggc cgccgcaggc accggcggca ttttgctggc gatgagcgca
      781 attgccacca ccacgaagaa ggatttcagc ttcatgggca aattcctgtt cgtgggcatg
      841 atcgtgctgc tgctcgccat gattgccaac atgtttttcc agattccggc gctggctctg
      901 gcgctttcgg cgctggtggt cgtggtgttc tcgctgttcc tgctgcacga cgtcagccgc
      961 atcgtcaatg gcggcgagac caactacatc atggcggcaa cgggcgtgta catgagcctg
     1021 ttcaacatat tcgtcaatct gctgcaattg ctcatggcat tctccggcga acgcgattga
     1081 ttctgccgtg catcaaggaa agggcaaccc ctcgggttgc ccttttttca ttccagttac
     1141 ccgttcaagg catcgcgaac aaggcaaccg attccacgtg cgaggtatgc gggaacatgt
     1201 tcaccacgcc cgccgcggcc agccgatagc cgtgtgaatg caccagcacc tccgcgtcgc
     1261 gcgccagcgt cgccggattg caggatacgt agacgatgcg tgccgggggc gatttgccaa
     1321 tcgccttgac cagctcgatg gccccgtcgc gcggcggatc gatcaggacc cggtcaaacg
     1381 gcgccagccc gctccagtct tctccggtga tggcaaacag gtcccgctcg acaaaccggc
     1441 accttgcttc cagcccattg cgcacggcat tctcccgggc gcgctgcagc agtccgggat
     1501 tgccatcgat accggtaacc tgtgcgccgg acctggcgat ggccagcgag aaatttccca
     1561 ggccgcagaa aaaatcgccg atgcgatcgc ccggctgcgg gtccagcagc gtgatcgccc
     1621 gccgcaccag cgcccggttg atctcgggat tcacctgggt aaaatcggtg ggattgaagg
     1681 gaaaagtcag atcgaaatcg ggaatccggt actgcaactg cggcgcatcc agcggaaaga
     1741 agggatacat tgaatccggg cccttgggct gcaggaacag ctgcacccga tgctctgcgg
     1801 cgaactgcct gagctcgtcc tggtcggaga cgtcaggcgg ctccagcacg cgcagcacca
     1861 gaacatcgac cgcatcgccg atggcgagct ccacctgagg tagacgctcg cggatggaca
     1921 gcgaggcaat caactgccgc agcggtacca acaacacgga gatgcgagcc ggcacgacct
     1981 cgcaagtctg catgtcggcc acgaagctgc tgtgacgttc gcgaaagccg accagcgcgc
     2041 ctttctttgc gacgtagcgc gccgagaagc gcgcgcggta gcggtactcc caggtcggac
     2101 catgaatcgg cgaaagcatg gtctcggccg tcaccttgcc gatacgggcc agattttcct
     2161 cgagcacgcg ctgcttcgcc gccacctgcg cacgcggctc caggtgctgc atgctgcagc
     2221 cgccacagac gccgaaatgg cggcaacgcg gcacgacgcg cgaggccgac gaacgcacga
     2281 ttctccttgc ctcggcgaac tcatagttgg gtttgcgccg gtaggatgcg aactcgacca
     2341 cttcgccggg gagcgcgcct tcgatgaaga ttaccttgcc gtccacatgg gcaacgcccc
     2401 gcccttcctg gtcgagggac tcgaccagca ccgcaggccc gacggaatcg cgtttcatgc
     2461 tagtgccctg tcccgctgat tcgtgggaca acttgcgaac cttggagggt agacctcata
     2521 ttcacttggc aacaatccat cggaacagga cactaggcct gccagccgtc caggaattcg
     2581 cgccagtgcg catccgtgga tttggagaac gtcgagcgca ccagatcgca ttcgcggcgg
     2641 tattgtgtct cgtcgagtgc accgcggatc aactggaagc gctgataaac gaggtaagtg
     2701 ttggccacgt cagtctcgca gtagtgcctg atcgcctcga tgctgcctgc ctggtaggcg
     2761 tcccagactt tagagccgtc catgccgagt tttcccggaa ggccgagcag ttgcgcgatc
     2821 tggtcgagcg gcgcattgcc acggccctga tacatcgcca gcacatccat caggtcgaga
     2881 tggcggctgt gatagcggct tatgtaattg ttccacttga agtcgcggtc atcctcgccc
     2941 atgtcccaga aacgcgcggc acgcacgccg tgaatcagcg cccggtaatt gagcaccggc
     3001 agatcgaagc cgctgccgtt ccaggaaacc agctgaggcg tgtacttctc gatgccatcg
     3061 aagaagcgct ggaccaggtc gtgttcgggt tcgcccgcct cgcccagcga ccagacgcgg
     3121 aaggtgtcgc gatcgcgcag cgcgcaggaa attgccacca cgcgatgcag gtgcagcggc
     3181 aggaaatcgc tgccggtggc ctcgcgccgg cgatcaaacg ccgcctgcgc cacttcggca
     3241 ggactggtct gcggtccatg gccgtacagg cgccgcagcc cttcgatgtc ggggatggtt
     3301 tcgatgtcaa atgccagaac aggggtcatg ccggtcggcg acgtttttca acccgcgtaa
     3361 acgtgccccg ggaggaaatc ccggattccg cacggtacaa acgcgtcagg ataccggaaa
     3421 gcacggcagt ccctccaccg gacttctttg cagtatgcgt tcggactccc tctcgatgtg
     3481 ccagcgtgat ctgccgttca cgcagcgcaa caacgcgcgg cgcgatcgga ctagggcggc
     3541 gatgaaactc cggtgctaga cttaccgtca aaacagtcgt gtcaggcgga ggagagctcg
     3601 tgaggtcgct ggcaagccaa accccggcgt tgcacaacgc catggattgt ccggactatg
     3661 tcaggaaccg gcaactgcgc gaatgggtag gcgaaatcgc gcagctcacc cgcccggacc
     3721 gcattcactg gtgcgacggc tcgcaggctg agtacgatgc cctgtgccac cagctggttg
     3781 ccggcggtac cttccgcaaa ctccactcga agaaacgacc ggacagttat ctggcgctgt
     3841 ccgatccttc ggacgtcgca agagtcgagg accgcacgtt catctgcacc acccgcagtg
     3901 aagacgccgg tccgaccaac aactggaccg acccgcagga aatgaagcag aggctgctcg
     3961 cgctgttcga cggcagcatg cgtgggcgga ctctttatat cgtgccgttc tcgatggggc
     4021 cgctcggttc gccgatctcg cacatcggcg tggagctcac ggattctgca tacgtggcaa
     4081 tcagcatgcg catcatgacc cggattggcc gcgaagcgat cgaagccctc ggcaccgagg
     4141 gcgggttcgt cgcctgcctg cactcggtcg gaatgccgct cgggcccggg caggaagacg
     4201 tacgctggcc atgcaacaag gaccacaagt acatcgtgca ttttccggag gagaaagcga
     4261 tatggtccta tggcagcggc tatggcggca atgcgctgct cggcaaaaag tgcttcgcgt
     4321 tgcgcatcgc ctcatgcatg gggcgccaac agggttggct ggcggagcac atgctcatcc
     4381 tcggggtgga gtcgccgcag ggcgaaaaga attacgtggc ggcggcgttc ccgagcgcgt
     4441 gcggcaaaac caacttcgcg atgatgatcc cgccggcggc attcaagggc tggaaagtca
     4501 ccacggtggg cgacgacatc gcctggatca agccgggtgc ggacggcagg ctgtacgcga
     4561 tcaatccgga gtacggtttc ttcggggttg cacccggcac ttcggaaaaa tccaatccga
     4621 acgcgatggc gacgctgagc cgcaactgca tcttcaccaa cgtggcgatc acgcccgagg
     4681 gcgatgtctg gtgggaaggc atgaccgaag agccgccggc gcaactcacc gactggcagg
     4741 gccttccctg gacaccggac tgcggacgca aggccgcaca cccgaacgcg cgcttcacgg
     4801 tgccggcgtc gcaatgtcct tcaatggatg ccgcgtggga gacgccggag ggtgtgccga
     4861 tatccgcgtt cgtcttcggc ggccgacgcg gcaccaccgt cccgctggtc accgagagtt
     4921 tcgattggac ggacggagtg tacatggccg ccacgctcgg ctcggaaacc acggcggcgg
     4981 cgaccgcgaa ggtcggtgag gtgcgccgcg atccgatggc gatgctgccg ttctgcggtt
     5041 accacatcgg cgactacttc gaccactggc ttggaatcgg tcgcggactg aagcatccgc
     5101 cgcggatttt ccacgtcaac tggttcagga aggacggcga cggcaagttc ctctggccag
     5161 ggtacggcga aaatatgcgg gtgctgaaat ggatcgtgga ccgttgccag gggcggctcg
     5221 aggccgccga aagcccgctg ggctggatgc cgcgcagaca ggacatggtg tgggacgggc
     5281 tgaaagtctc gaacgagacg ttcgacgaat tgatgtcgct ggaccgcgag gcctggaccg
     5341 aggagctgcg actgcacgaa caactgttcg cacagatcca ggatcgcctg ccgcgggaac
     5401 tcgcgcagaa acgcgaactg ttcctcgccc gcctgtccgg cgcgccagcg cgctggaccg
     5461 tgcctttttg aaaggccctt ttcaccgcaa agacgcaaag gacacgaaga agaacacgcg
     5521 accaaccata aagttaatca ccaccggaaa aaaatgccgt tgccgacgca tggcagcatg
     5581 cattttctcc tgtgcgattc ttgcttttct ttgcggtctt tgcgactttg cggtgaggtg
     5641 tttggtgagc tattcggcgg ggaacacgcc ggtggagaga tagcggtcac cgcggtcaca
     5701 gacgatggtg acgatggtgg cgttctccac ttcccgggaa acctgcaacg ccgcccacag
     5761 cgcgccgccg gaggagatgc cggcgaaaat cccctcttcc tttgccagcc ggcgcgtcat
     5821 ctcctcggag tcggcctggc tgacttcgat aacccggtcc acgcgcgtgc ggtcgtatat
     5881 tttcggaagg taggcctccg gccacttgcg tatgccagga atctgggaac cctcggtcgg
     5941 ctgacagccg aagatctgga tcgcgggatc cttttccttg aagaaacgcg agcaacccat
     6001 gatcgtgcct gtggtgccca tgctgctgac gaagtgggtg atctggccgc gggtgtcgcg
     6061 ccagatttcc gggccggtgc cttcgtaatg cgaaagcgga ttgtcggggt tggcgaactg
     6121 gtcgaggatc aggccttcgc cgcgcgcctg catctgctcc gctatgtcgc gtgccgtttc
     6181 catcccgcct tccttaggcg tcagtacgaa tctggcgccg aacgccgcca tggtctgccg
     6241 ccgttcgacc gacaaatgct cgggcattac cagtaccatg cggtaaccgc gcatcgccgc
     6301 caccatcgcc agtgcgatgc cggtgttgcc gctggtcggt tcgatcagcg tgtcgcccgg
     6361 tttgatctgc ccgcgttcct ccgcgcgccg gatcatggat agcgcaggac gatccttcac
     6421 cgatccggcc gggttgttgc cctctagttt ggcgaggacg acgttgctgg tcctgccggg
     6481 gatgcgcttc aggcgcacca gcggcgtatt gcccacgaag tcgtcgaggg tctgataggc
     6541 actgacggga ttgcgatcca tggaaatatg atactcgcgc ggtcctggga gcggagaatt
     6601 gcttgaatgt ggctgccgaa attcctatgg cgcggcgtcg agcagccatt cgacgtaacg
     6661 cgtgacgccg gtttcgacgt cgaagaacgg tgcggcgtat ccgcagccgc gcagttgcgc
     6721 gaggtccgct tgggtgtagc tctgatattt ttccctgagg ccggcgggaa aggatacgta
     6781 ctcgatcatt ccctgcgacc tgagttccgc aagactgtgc gggttcttgc cttcggcacg
     6841 ccgcaaggcg ttgatggtcg cgaccgcgat atcgttgaag gtctgcgaag cgccggtgcc
     6901 gcagttgaaa atgcccgtct tcctgggatt gtcgaggaaa tacaggttca ccttcaccac
     6961 gtcctcgatc gagatgaaat cgcgccgctg ctcgccagcg gcgtacccgc cgctgccctc
     7021 gaacagtttc acctttccgg tctcccggaa ctggttgaag caatggaatg cgacagaagc
     7081 catgcgctcc ttgtgcgact cccgaggacc atagacgttg aagtaacgca gcccggccac
     7141 ctgcgccgtg ttgtcctccc agcgccggcg caccaactgg tcgaagagaa acttcgaaaa
     7201 accgtagacg ttcagcggtt tctcgttttc cctgccctcc ttgaataccg cccctgatcc
     7261 gtataccgac gccgaagatg catagatcag cggcaccgac tccgactggc agtatgccag
     7321 caggtccacc gagtaacggt aattgttctc catcatgtag cgcccgtcgg tctgcatcgt
     7381 gtccgaacag gcgccctggt gaaggatggc cgcgatttcg ccgtcgaagg aaccggcatc
     7441 gagcgccgcg cggaagtgtt ccttgtccag gtagtcggcg atctcgcaat ccgccagatt
     7501 gcggaacttg tcggcacggg tgaggttatc caccgcgaga atgccggtct cgccgcgcgc
     7561 gttcagcgcc ttgacgacat tcgagccgac aaaacccgct gccccggtca caacgatata
     7621 catgcgaaca atccagaaac gctaatagtg agggataaag gatcaaggat aagggatgaa
     7681 ggatgaggga tgagcgatga agtgtcatgc ttcgcgcctt ctccccttcc ttcagccttc
     7741 atccttccgc cttcatcctt gctcttcgaa caattcttcc gggtgaacca ccgcggtacc
     7801 gagcttgcct actacgattc cggccgcttt gttggcgaac tgcacggctt cagcgaatcc
     7861 ggcgccggcc gccagcatca cgccgagaac ggcaatgacg gtgtctcccg cgccgctgac
     7921 atcgtacact tcgcgtgcca ccgcgggcac atgcagccgc tgctggtcgc gatacagggt
     7981 catgccttct tcgctgcggg tgaccaacag cgcatccagc cccagttcgc ggcgcattat
     8041 ctgcgctctt tcggtgagat cgtcttcgct tttccagcgt cccgccactt cacggaattc
     8101 ggcgcggttg ggcgtgacca gcgtcgcgcc gcgatagcgg ccgtaatcgt ctcccttcgg
     8161 atcgaccagt accggctttc ccgaaccacg cgccaggtcg atcatcttct cgatatgcct
     8221 gagcccgccc ttgccgtagt cggaaaggat caccaggtcc gccttcggca gccgcgactc
     8281 gaagtcggcg agcttggaca tcagcacttc atgccctggc tcggtttcga aatcgatgcg
     8341 aagcaactgc tgctggcggc cgatcacgcg caacttgatg gtggtagcga gcccgggatc
     8401 gcgatgcaac tgggcggtga tgccaacgga ctcgagcagg cgctggaggc aatcgccggc
     8461 ttcgtcctgc ccgaccaccg acagcagcga agcttgcgca ccgacggcaa cgatattgcg
     8521 cgcgacgttg gccgcgcccc cgaggcgttc ttcggtgcgc cccactttca ccaccggcac
     8581 cggcgcctcg ggagaaatcc ggctcacctc gccgaaccag tagcggtcga gcatgacatc
     8641 gcccactacc agcacccgcg cggaacgcaa ggatgaagga tgagggatga aggatgagga
     8701 gctcatttca acgccttgtg tttttcgctc ccgccttgtc tcaccggttt tccccttcat
     8761 ccttccgcct tccgccctca tccttgccct tcagccttca tccttccgcc ttcacccttg
     8821 tccttcatgg tcttcccacc gagaaatact cgaatccggc ttcccgcatc aaaaccggat
     8881 cgtacaggtt gcgcccgtcg aacacgatcg gcgccttgag gcgtcgcttc atgtcgtcga
     8941 aatccgggct gcgaaattcc ttccattcgg tgacgatgat cagcgcatcc gccccatcga
     9001 gcgcggccgt ggtggacggg gcgaggtgca ctgccggttc tttcgcgaag accagcttcg
     9061 cctccttcag cgcgaccgga tcatagacgt ttacgctcgc gccgcgggcg agcagaccat
     9121 tgatcaccac gcggctcggt gcctcgcgca tatcatccgt gttgggcttg aaagacagcc
     9181 cccacacggc gaacctgcgg ccgtcgagat gttcgccgaa acgcattcgc accttctcca
     9241 ggatgcgctg cttttgcgtg cggttcactt tctcaaccgc atccacgatg gtcatttgca
     9301 tgccgtactc gccggcggtg tgctgcagcg cctggatgtc cttgggaaag cacgatccgc
     9361 cgtagcccac tcccgcataa aggaagtgat agccgatacg ctggtcggag ccgattccat
     9421 gacgcaccgc ttcgatgttt gcaccgagtt tttcggcgag attggccagc tcgttcatga
     9481 aggaaatgcg ggtggcgagc atcgcgttgg cagcgtactt ggccagttcc gccgacctgg
     9541 ggtccatcac cagaaggcgg tcgtgattac gcaggatcgg cgcgtacaac tggcgcatga
     9601 tctgcgtggc gcgctcgtcg ctgctgccga ccacgatacg gtccgggcgc atgaaatcgt
     9661 tgaccgccgc gccttccttg aggaactccg ggttggaaac cacgctgaac ggcagcgtca
     9721 cgcggcgggt cttcaattct tcttcgatca ccgcgcgcac ccgttccgcg gtgcccaccg
     9781 gcaccgtgga cttgttcacc accacgcggt aatcgtccat atgacggccg atgctgcggg
     9841 cggcgccgag cacgtagcgc agatccgccg agccgtcctg gtcggccggc gtgcccacgg
     9901 agatgaattg caggctgccg tgcgcgacgc tttcctctac atcggtggtg aagcgcaatc
     9961 gacctgccgc ggcgttgtgt ctgaccagtt gctcgagtcc cggctcgtag atgggaacac
    10021 cgccggagtt gagcgccgcg actttgtgct cgtcgaggtc gagacagacc acatcgttgc
    10081 caagatcggc aaaacaggcg cccgatacca gtccaacgta tccgctgccg atgatggtga
    10141 tcttcatttt ccgttctcca gtaacaccac ttccgcatta atgcgcgcta gcacttcaag
    10201 aggctgcgcc gcacgcgtga caggccggcc gatgaccaga tagctcgaac cggattcgag
    10261 cgcctggcgc ggcgtcatta cccgtttctg gtcttccgcc gagtcttcgg ggagacggat
    10321 tcccggcgta acccgcagga atgcttcgcc caggtgcgcg cggatcgcgc cggcctcgaa
    10381 tgccgcgcac accacgccgt ccaggccgca ccgctttgcc agcgcggcaa gacgcagggc
    10441 ttcttcgagc ggcgtggcat cgattcccac ttcatgcaaa tccgtctccg ccatgctggt
    10501 caacacggtc acggcgatca atcggggccg gccgggcgcg gtcccgatcg cgtcgcgcgc
    10561 cgcctgcatc attgcccggc cgccgagcgc gtgcacgttc agcatccaca cccccagacc
    10621 ggccgccgtc ttgcaggact gcgccacggt gttcggtatg tcgtgaaatt tcagatcgag
    10681 aaagatatcg aaaccgcgtt tcacgagcat tgcgacaaat tccggtcctg ccgcggtgaa
    10741 caattccttg ccgaccttca gccggcagac ggagggccgc aggcgatcaa ccaaagccgt
    10801 tgccgaccgc gcgtccgcgt agtcgagcgc gacgatgatc cgggaaccgg ttgtcaagaa
    10861 tccagctccc gttcttcggt gcgcctgggg gagtaggttt cccagccgtt gcaggctggg
    10921 cagtgccagt agaactggcg cgctttgaaa ccgcaattgt cgcagcggta gagtgcgagg
    10981 ctcctggtgt gctggtgcac caggttcttg atcaactgga gatcctggcg gcgttcggcg
    11041 ggcacctcca gaagctgggc ttcgagcagc ttgtccagtc cgagcagcgt ggggttgcgc
    11101 gccagctcgt cgcgcaccag acggtatgcc gaatccgcac cgcggtgctc gatctctccc
    11161 tgaaaagtgg cgttgagcag gtcgagcgac ggatagctgc ccagatagcc gcgcagcaac
    11221 tgcagtcctt catcctgccg tccgaggttg cgataggcaa gcagcagctt gtctgcaatc
    11281 agcgagagat acgccggatt ctgcccctcg atgcgtttcc aggcctcgat cgcccgctcg
    11341 ttgttgtgtt gggtcagttc tatgtcgcca agcaggatgc tggcgcgcgc gcacttgcgg
    11401 ttgacgttga gcgcctcccg cacatagtcg cgcgccgccg cccaacgtga atgggtcatc
    11461 tctccaaccg ccaactcgca gtagaaattg gcgatatcca cggcgctcga ctgaccggcc
    11521 accgcatcca tcctgcgcga agcgtcgatc gcttttttcc agtccttctc ctgttcgaag
    11581 atttccagca ggaacttcag cccctgctgc tcgtactgcg tgccctggag ctttccgaac
    11641 atctcctcgg cgcgatcgag caagccggcc ttcagatagt cctgcgccag ctcgtacagc
    11701 gcagtgagct tctggtccgg cgccagatcg ggccgatcga gcaggttttc gtgcattcgg
    11761 atcgccctct ccacttcgcc gcgacgccgg aacaggctcc ccagtgcaaa atgcagctcg
    11821 atggtctgcg gatcgacttt caccacctct atgaaggctt cgatcgcctt gtccggctgc
    11881 tcgttgagaa gaaaattcag gcccttgaaa taggaaagcg ggagcgcgcg cgactcggac
    11941 aacagttgct tgatgtccac ccgcgccgcc agccagccga gcgcgaagaa cagcgggaat
    12001 ccaagcaacc accagagttc aaattccatc ggctaacgcg cgtcctctgg cgcagtcgcc
    12061 ttcgcccgca attccttgcg taactgcaga atctcgcggc gctgacggta gaagtaagtc
    12121 agacaggcaa tcacgcctgc cagcgcaccg acgccgaaaa agaccagcac taccatggcc
    12181 agcggcgcct cccactggct gccgagatag aaacgcacga ccacaggatc ggtgttcttc
    12241 agcgcgaacc cgaaaagcag cacgaacagc aggattcgca atgcccagga gatatagcgc
    12301 atcagcggcc ccaggaagcc agggggcgag agtcagacga aaaaaagcgg cacgatcctg
    12361 cgacgatgcc gcctgcattg tggcgccggt cgaacacgcc gccgcctact ggctcttgaa
    12421 atccacgcgt tcgcgcaatt ccttgcccgc cttgaaatgc ggcacgtact tggccggcac
    12481 gtgaaccttg tcgcccgatt tcggattgcg gcccacacgc ggggggcggt aattcaatcc
    12541 gaaactcccg aatccgcgaa tttcgatgcg gtgaccctgc gccaggcttt tcgccatggc
    12601 atccagaatc atcttcaccg caagctcggc gtcgctcgcg accaactggg gaaagtgcgc
    12661 cgccagcttg gcaatcagct ccgacttggt catggcgcgt cctattgcgc agagttcttg
    12721 ttatccagct tggccttgag caaggcccca aggttggtcg tgcccgcatt cgacgcgctc
    12781 tcggcggcat gtttctgcat cgcctcggac tcgtcggccg catccttggc cttgatcgag
    12841 aggttgatgt tgcggttctt gcgatcgatg ttgatgatca ttgcattcac cttgtcgcct
    12901 tccttcaggt gcgtgcggat gtcctcgacg cggtcgcgcg cgatctcgga ggcgcgcagg
    12961 taaccctcca cgtcggtgtc gagctgaact acggcgccct tgggatctat ggtcttgacc
    13021 acaccatcaa cgaccatgcc cttgtcgtgc gtggcgacga aattggtgaa cgggtcgccc
    13081 tcgagctgct tgatgcccag agaaatgcgc tcgcgctcga cgtcgatgga aatgaccatc
    13141 gcttcgactt cgtcaccctt cttgtaattg cgcaccgcct cttcgccggg aacgctccag
    13201 gaaagatcgg acaggtgaac caggccgtcg atgccacccg gcaggccgat gaagattccg
    13261 aagtctgtga tcgacttgat ctggccacgg accttgtcgc ccttcttgta attggcggag
    13321 aactcttccc agggattggg tttgcactgc ttcatgccca gcgaaatgcg gcggcggtcc
    13381 tcgtcgatct cgagaatcat cacctccacc tcgtcgccga gttgcaccac gcgcgagggg
    13441 tgaacgttct tgttggtcca gtccatctcg gacacgtgca ccagcccctc gatgccctgc
    13501 tcgatctcga cgaacgcgcc atagtcggtg aggttggaaa ccttgccgaa caggcgggtg
    13561 ccgggagggt agcggcgcga caggccgacc cagggatctt cgccgagctg tttcaaaccc
    13621 agtgaaacgc gattcttttc ctgatcgaac ttgaggatct tggcggtgac ttcatcaccg
    13681 acattgagaa cctcggaggg atgcttgacg cggcgccacg ccaggtcagt gatatgaagc
    13741 aggccgtcga tgccgcccag atcgacgaac gcgccgtaat ccgtgatgtt cttgactatg
    13801 cccttgaccg tggcgccttc ctgcaggctc gcaagcagcg cttcacgttc cgcgccctgg
    13861 ctggcttcca gcacggcgcg gcgcgatacc accacgttgt tgcgcttgcg gtcgagcttg
    13921 atgaccttga attcgagatt cttgccttcg taggggttgg tgtccttcac cgggcggatg
    13981 tccaccagcg aaccgggcag gaaggcgcga atgccgttca ccatgaccgt gagcccgcct
    14041 ttgaccttgc cgttgaccag gccctgcacc aaggtaccct gttccatcgc cttttccaga
    14101 tcgtgccagg cggccaggcg cttggccttt tcccgcgaca ggcgggtttc cccatagcca
    14161 tcctcgagcg cttcgatggc gacggtcaca tagtcgccgg gcttgacctc gagttcgccg
    14221 cggtcgttct tgaattcgtc tgccgagatg aagctttccg acttcaggcc ggcgttcacc
    14281 acgacgaaat ttggatctac acgcaccact tcggcggtaa tgacttctcc tgcgcgcatc
    14341 tcctgtcgcg acaggctttc ttcgaacatc gcggcaaaat tattctcgga agcggcggcg
    14401 tcaggaatta gggtttcggt tgtcataaaa agagggttgg actcgcaaaa atccgttgct
    14461 taaaacgggg gttagttaat gaacccgtcg acggcggaaa aaagacccac ctggggcatg
    14521 caactgccgg cagtttgcag gtctgattca gcgggacggg gcccggcgct gcgcagattc
    14581 gaaccactgg acgatctggg cgactgcgtc ttctattgaa agcccagtgg tgtcaagaac
    14641 ttgcgcgtct gcgctctttt gcagaggagc cacgctgcgc aagctgtcgc gggcgtcgcg
    14701 ttggctgatt tcctgcaaaa gggtggctag attagcatcg aaacctttgt ctatcaactg
    14761 tttatagcga cgcagcgcac gctcctcggc ggacgcggtg aggaacacct tgagtattgc
    14821 atcgggaaac accaccgtgc ccatgtcacg cccgtcggcg accagtcccg gaggccggcg
    14881 aaatgcccgc tggcgcttga taagtccttg ccgcacttcg tgaattgccg caacgtggga
    14941 ggcggccgca gagacttctt cggtgcgaat ggcgtcggtc acgtcgtctc cgtcaagacg
    15001 aatgttgtcg ccgtgaaact cgactggaac agattgggcg agccgtccga gcgcgccggc
    15061 atcgtccatc gcgattcctt ggcgcattgc ggccagcgcc accacccggt acagcgcacc
    15121 gctgtcgaga tagtggaatc cgagtcgctg cgccacccgc cgcgcgactg tgccttttcc
    15181 cgatgccgac ggcccgtcga tggcgataac cggcaccgtg gcgccgccgg caatctcggc
    15241 aaatcgatcg aaaaaatccg gaaaggtctt
//
LOCUS       DASQOP010000104         5055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_848757, whole genome shotgun sequence.
ACCESSION   DASQOP010000104 DASQOP010000000
VERSION     DASQOP010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5055
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848757"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..1552
                     /locus_tag="VE030_04275"
     CDS             119..1552
                     /locus_tag="VE030_04275"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HZF22668.1"
                     /translation="MLWAAVHLPDLSLQLRLRGTVAPGPVVIQDQSHRPRVLSCNDAA
                     TGLGIRPGMPVSAAFALAAGLQLFTRDPEKEEKALKGVALWAGQFTPTVSIASGNEVL
                     LEIGGCLRLFGGLRQLGGRIRANLRELGYHAVVAVAPTATGALLLSRAGIATSVGDRH
                     ELRRALAPLRLRLLDQPADTVATLAGMGVHTVRDYLALPRDGLARRFGQRLLDEIDRA
                     LGHLPDPRPPFIPPARYATALELPAPVQETEPLLFAAKRLILELAGYLAMKQVGVTQL
                     RFDLEHADHSITRIVVGLSAANRDAGHLITLLREKLATVELPAPVETIRLIAAETRPL
                     GSHNHSLFADRRPAAEDRWRVIEHLRARLGADAVYGLGMFPDHRPELALRRVPAGKAQ
                     DGDCDLYRPLWLLARPLPLKAGDLLPQTDGPLVLLDGPERIESGWWDDFDVTRDYFVA
                     RDDSGAKLWIFRERNDQKNWFLHGVFN"
     gene            complement(1624..2007)
                     /locus_tag="VE030_04280"
     CDS             complement(1624..2007)
                     /locus_tag="VE030_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZF22669.1"
                     /translation="MLNTAPTTTILPVKDMNRARSFYEKTLGLKPLGFAADGNFMFAC
                     AGRSTIALIPKPQGTKAEHTALSFEVEDVVKEIKELKQRGVVFEDYDLPGFKTVEHVC
                     VVGSDKAAWFKDPEGNILCVHETRS"
     gene            2237..>5055
                     /locus_tag="VE030_04285"
     CDS             2237..>5055
                     /locus_tag="VE030_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HZF22670.1"
                     /translation="MDRAVQLGYQALALTDECSFAGVVRAHKAAKDTRLKLIIGGEFR
                     LDDGLRFVLLATNREGYGNLSELITRGRRNAEKGEYRLSRADLGPGLEHCLALWLPPA
                     QPQPEDAHWLAERFPGRTWIAVELLARGGDWDRLDALREMSRETRLPCVACGDVHMHV
                     RSRRALQDTLTAIRLGVQVAQAGRALLPSGERHLRRRETLERIYPKEWLEETIRIAGL
                     CNFSLEELRYEYPEEIVPENKTPAQYLRELTEEGLMWRFPPPGTRDEGRGTRENRQTQ
                     RKDVEENGSGCENELLITTVPPFPQHSALVPRPSPLAPRAEAVPAKVRALVEHELALI
                     AELRYEAFFLTVYDVVQYAHRQKILCQGRGSAANSVVCYALGITEVDPTRTRLLFGRF
                     LSKERNEPPDIDVDFEHARREEVIQYVYRKYGRHRAALAATVISYRTRSAFRDVGKAL
                     GLDSAQLDRISGSFVWWDRPEDRAARLREAGFEPCSSVIRKLIALTDTLIGFPRHLSQ
                     HVGGFVISRGPLSRLVPTENAAMPGDPSQYTDDGIKPPSKDVAAASADPLRTNIQWDK
                     DDLEELGLLKVDVLALGMLTAIRQALGLVNRRRGTALSMADILVTEDGDEGKPVYDML
                     CKADSIGVFQVESRAQMSMLPRLRPRNYYDLVIEVAIVRPGPIQGGMVHPYLSNRKKG
                     PGKIGYPSPELEKVLERTLGVPIFQEQVMEIAMVAADFSPGKADQLRRAMAAWKRKGG
                     LEPFKDDLVNGMCKKGYDEKFALRIFEQIKGFGEYGFPESHSASFALLVYVSAWLKYY
                     EPAAFTCALLNSQPMGFYQPAQLVQDAQRHGVEVRPVDVLVSEWDCTLEPRSGSGVRG
                     EGRGTRENQEPQCGSGTRHEARGAREERVLNTAPCIPRDSTSQSLSLAPRPSSLAPDS
                     LSLTPDGQPAVRLGLRMISGFSQEAA"
BASE COUNT          924 a         1639 c         1560 g          932 t
ORIGIN      
        1 agccaatgaa cggttttaac ccgagaatgt ttttcccctg gcctttcctc cgtgtcctct
       61 gtgtcctccg tggttcaaga ttttttcctg atttgtgatt tatcaagaat gcgccgccat
      121 gctgtgggcc gccgtccatc ttcccgactt gtcgctgcaa ttgcggctgc gcgggacagt
      181 cgcgcccggc ccggtggtga tacaggacca gagccatcgc ccgcgcgtgc tgtcgtgcaa
      241 cgatgccgca accgggctcg gcatccggcc cggcatgccg gtatcggcgg cgttcgcgct
      301 cgccgcagga ctacaacttt tcacccgaga tccggaaaaa gaagagaaag cgcttaaggg
      361 cgtggcgctg tgggcgggac agttcacgcc cacggtgagc atcgcctccg gcaacgaagt
      421 actgctcgaa atcggcggct gtctcaggct gttcggcgga ctgcgccagc ttggcgggcg
      481 catccgcgcc aatctccgcg aactgggcta tcacgctgtg gttgccgtcg cgcccactgc
      541 caccggtgcg ctgttgctct cccgcgccgg catcgccact tccgtcggcg accgtcacga
      601 attgcggcgg gcgctggcgc cgctgcggct gcggctcctc gaccagcccg cggacaccgt
      661 cgctacgctc gcgggaatgg gcgtgcatac cgtcagggat tatctggccc ttccgcgcga
      721 cgggctggcg cgccgcttcg gccagcggct gctcgacgag attgaccgcg cactcggaca
      781 ccttcccgat ccgcgcccgc ctttcatccc gcccgcacgt tatgcgaccg cgctcgaact
      841 gcccgcgccg gtgcaggaaa ccgagccgct gctgttcgcc gccaagcgcc tgatcctcga
      901 actcgccggc tatctcgcca tgaagcaggt cggcgtgaca caactgcgat tcgatctcga
      961 acacgcggac catagcatca cccggatcgt ggttggactg tcggcagcga atcgcgatgc
     1021 cggtcatctg atcacgctgc tgcgcgagaa actcgccacc gtcgagttac ccgctccggt
     1081 cgaaaccatc cggctgatcg ccgcggaaac ccggccgctc ggttcacaca accactcgct
     1141 gtttgccgac cggcgaccgg ccgcggaaga ccgctggcgc gtcatcgagc atctgcgcgc
     1201 gcgcctcggc gcggatgcgg tatacggcct gggaatgttt cccgaccatc gcccggagct
     1261 tgcgctgcgc cgcgtgcccg cgggaaaagc gcaggacggc gattgcgatc tttaccgccc
     1321 gctgtggctg ctcgcccggc cgctgccgct gaaagccggc gatctgctcc cgcaaacaga
     1381 cgggccgctc gtgctgctcg acggaccgga gcgcatcgag tccggctggt gggacgattt
     1441 cgacgtgacg cgcgactact tcgtcgcacg cgacgattcg ggagcaaaac tgtggatctt
     1501 cagggaaagg aacgatcaga agaactggtt cctgcacggc gtattcaact gacctcgcaa
     1561 gcacccatgt ccgagcgtgc cctcatttcc gcgaacgccc tgtccgccgg cagtgcccgt
     1621 ggctcagctc ctggtctcat ggacgcaaag gatgttgccc tcggggtcct tgaaccacgc
     1681 ggccttgtcg gatccgacca cgcagacgtg ctccaccgtc ttgaaaccgg gcagatcgta
     1741 gtcctcgaac accacgccgc gctgcttcag ttcctttatt tccttcacca cgtcctctac
     1801 ctcgaagctc aacgccgtgt gctcggcctt cgtaccctgg ggcttgggga tgagcgcgat
     1861 ggtggatcgc cccgcgcacg cgaacatgaa attgccgtcg gccgcgaagc ccagcggctt
     1921 caggcccagc gttttctcgt agaagctgcg cgcgcggttc atgtccttca ccggcaggat
     1981 ggtggtcgtc ggtgcagtgt tcagcacggt cttctccttt ccgttctatc ggcgggaacg
     2041 cgcgcctcac gcgggcgtcg aggccggatt cagggacagc gtcctgtctg gttataggta
     2101 acgttcccgg ctgccgcaag gcggtttgcg ggctttttgc aaaagcgacc gttctcacat
     2161 gccgcaggct tacgccgaac tacattgtct ttccaacttc accttccagc gcggtgtctc
     2221 ccacccgaag gagctggtgg atcgagccgt acaactcggt taccaggcgc tcgcgctcac
     2281 cgacgaatgt tcgtttgccg gcgtggtacg cgcgcacaag gccgcaaaag atacccgact
     2341 gaagctgatc atcggtggcg agttcaggtt ggacgacggc ctgcgctttg tcttgctcgc
     2401 gaccaatcgt gaaggctacg gcaatctttc ggagttgatc actcgcggca ggagaaacgc
     2461 cgaaaaaggc gaatatcgtc tttcgcgcgc agacctcggc ccgggactgg aacactgtct
     2521 cgcactctgg ctaccgccgg cgcaaccaca gcccgaagac gcccactggc ttgcggagcg
     2581 tttccccggc cgcacctgga ttgccgtcga actgttggcc cgcggcggcg actgggaccg
     2641 gctcgatgcg ctgcgggaaa tgagcaggga aacccggctc ccatgcgtgg cctgcggcga
     2701 cgtgcacatg cacgtgcgca gccgccgcgc gctgcaggac acgctgaccg caatccggct
     2761 gggcgtgcag gtggcccagg ccggccgcgc gctgctgccg agcggcgagc gccatctgcg
     2821 ccggcgcgag acgctggagc gtatctatcc gaaagagtgg ctcgaggaga ccatccgcat
     2881 cgccgggctg tgcaattttt ccctcgaaga attgcgctat gagtatccgg aagaaatcgt
     2941 tccggagaac aagacaccgg cgcagtacct gcgggaactg accgaggaag gactgatgtg
     3001 gcggttcccg ccaccaggga cgagggacga ggggcgaggg acgagggaaa atcggcaaac
     3061 ccaacgcaaa gacgtagagg aaaacgggag cgggtgtgaa aacgagttat tgatcacgac
     3121 agtcccgcct ttcccgcagc actcagcact cgtccctcgt ccctcgcccc tcgcccctcg
     3181 cgctgaagcc gtgccggcga aggttcgcgc gctggtcgag cacgaactcg cgttgattgc
     3241 ggaattgcgc tacgaagcct tcttcctgac cgtttatgac gtcgtccagt acgcccacag
     3301 gcagaagatt ctctgccagg gccgcggctc tgccgccaat tcagtagtct gctatgcgct
     3361 tggaatcacc gaggtcgacc cgacccgcac caggctgctg ttcgggcgct ttctgtcgaa
     3421 ggaacgcaac gaaccaccgg acatcgacgt ggatttcgag cacgcacgcc gcgaggaagt
     3481 gatccagtac gtttaccgga aatatggccg gcatcgcgcc gcattggccg caacggtgat
     3541 cagctaccgc acgcgcagcg ccttccgcga cgtcggcaag gcgctgggcc tggactcggc
     3601 ccagctggac cgcatctcgg gcagctttgt ctggtgggat cgtccggaag acagagcggc
     3661 aagattgcgc gaggccggtt tcgagccttg ctcctccgtg atccgcaagc tgatcgcgct
     3721 gaccgacacc ctgatcggat ttccccgcca tctgtcgcag catgttggcg gcttcgtgat
     3781 ctcccgtgga ccactgtcgc ggctggtgcc gaccgagaac gcggccatgc ccggggatcc
     3841 atcccaatat accgacgatg ggatcaagcc cccatcgaag gacgttgcag ccgcttctgc
     3901 ggacccgttg cgcaccaaca tccagtggga caaggacgat ctcgaagaac tcggcctgct
     3961 gaaagtggac gtactggcgc tgggcatgct gaccgcgatc cggcaggcgc tgggtctcgt
     4021 caaccgccgc cgcgggaccg ccctttccat ggctgacatt ctcgtcaccg aagacggcga
     4081 tgaaggcaag ccggtgtacg acatgctctg caaggcggac agcatcggcg tgttccaggt
     4141 cgaatcgcgc gcgcagatgt ccatgctgcc gcgcctgcgg cccaggaatt attacgactt
     4201 ggtgattgaa gtggcgatcg tgcgccccgg cccgatccag ggcggcatgg tccaccctta
     4261 cctgagcaac cgtaaaaaag gtccagggaa aatcgggtat ccgtcacctg aactcgaaaa
     4321 agtcctggag cgcaccttgg gcgtgccgat cttccaggaa caggtgatgg agatcgccat
     4381 ggtcgccgct gatttttcac cgggcaaggc cgatcagttg cggcgcgcga tggcggcctg
     4441 gaaaagaaaa ggcggactgg aacccttcaa ggacgatctc gtcaacggca tgtgcaagaa
     4501 aggttatgac gagaaattcg cgctgcggat cttcgaacag atcaagggct tcggtgaata
     4561 cggttttccg gaatcccatt cggccagttt cgctctgctg gtctatgtct ccgcctggct
     4621 caaatactac gaaccggccg ccttcacctg cgccctgctc aacagccagc cgatgggttt
     4681 ctaccaacct gcacaactgg tgcaagacgc gcaacgccac ggcgtcgaag tgcggccggt
     4741 ggatgtgctc gtcagtgaat gggactgcac gttggagccg cggagcggct caggggttag
     4801 gggcgaggga cgagggacga gggaaaatca agagccgcaa tgcggctcag gtacgaggca
     4861 cgaggcgcga ggcgcgagag aagagagagt actgaatact gcgccctgca taccacgaga
     4921 tagtacgagt caaagtcttt ctctcgcccc tcgcccctcg tccctcgccc ctgattcttt
     4981 atccctcacc cctgatggcc aaccggccgt ccggctcggc ctgcgaatga tttccggctt
     5041 ctcgcaggaa gccgc
//
LOCUS       DASQOP010000105         3213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_848818, whole genome shotgun sequence.
ACCESSION   DASQOP010000105 DASQOP010000000
VERSION     DASQOP010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848818"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(396..1151)
                     /gene="istB"
                     /locus_tag="VE030_04290"
     CDS             complement(396..1151)
                     /gene="istB"
                     /locus_tag="VE030_04290"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HZF22671.1"
                     /translation="MNQIELERALRQLRLGGIAAVLETRLRQAQAEAMAPIDLISSLV
                     SDELSRRSERLLDRRRKQAAFRDPHKTLDNFDFTFNPKMNRSLVFDLATCAFIGKRED
                     GLFLGPGGTGKSHLAQAIGQAAIQQNYRVLYRETHVLLDELAEAVVDGTRKAFMESVA
                     TVPLLIIDDFGMRKLPLTAAEDLLEIIMRRYERVSTLLTSNRPVEDWGKLLGDVAAVT
                     SMLDRILHHGHVLKCGPRSWRTKAAVTTAGDTQ"
     gene            complement(1148..2800)
                     /gene="istA"
                     /locus_tag="VE030_04295"
     CDS             complement(1148..2800)
                     /gene="istA"
                     /locus_tag="VE030_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HZF22672.1"
                     /translation="MSNVLNEEKKQQVIALGRLGWSLRRIQKNTGVRRETAAAYLKAA
                     KVAFRPPGGWGRQSPAKPANEVITDSAAGKPVSGAAADPVLAKPANEVTTDFGAGLAA
                     GLTVVPDSPPGRSPSASACWPYREAIELGLSRGRNAMAIWQDLVDTCSFAAGYQSVRR
                     FVSKLHPSPWREACAVIETAPGEEAQVDYGTGPMVRDPHSGKYRRTRLFVLTLGYSRK
                     SVRLLVFRSSSQVWAELHEKAFRRLTGVTRVVVLDNLREGVLTPDIYDPTLNPLYRDV
                     LRHYGAVPLPCRVADPDRKGKVESGVGHAQKTPLKGLRFESLEDAQTYLDHWEARWAD
                     TRIHGTTKRQVAVMFAEEKPLLLPLPVEPFRYYQYGERTVHLDGCVEVEAAYYGAPPG
                     WIGRHVQVQWDLRQVRLMNPETGQLLREHLRQVRGGHRIKDEDRPKHTPLGTLALLCR
                     ADKAGSQIGALCRGMHGEHGETMVRRIQGVLSLAKKYGAPSVEEACAAALELGVCDYR
                     FVRRYLERHPQMPLSLRQVDPLIRQLTLYRDFIENKTKESEL"
     gene            2857..>3213
                     /locus_tag="VE030_04300"
     CDS             2857..>3213
                     /locus_tag="VE030_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22673.1"
                     /translation="MAGFQVIINGRFWVITEGLSFGIAADETDKSDSIEVHMFLLFCP
                     FVSGTGKRVGAAPKTRSCFSEGTGTGEPEPEGCESKAEASQTPGAAKAPKQCRDNEQD
                     RKRDRTDHHFAGRRRWR"
BASE COUNT          567 a          993 c         1009 g          644 t
ORIGIN      
        1 ctgcgagccg acctgaaatc ctattatccg caggttttgc agtggttcga ggatctctac
       61 acaccggtgg cgctggattt cttagagcgg tggccaacac tggaggactt acagaacctc
      121 ggtgatcacc tcaaatccgg ccacctgtga tcacttcaaa agcggccaac gaagccgcac
      181 ccaggacatg aagttgtttt accgcgccgg caccggttcg ggcaagtttt gttcggtctc
      241 cctctgatgc gtcctgctgg gtcccgacca gcgggagggc gcagaatctt ctttgttttt
      301 ccttctaaga aaggtttttg ttttggtttt cggtggggtc gcaggggcga agcccccgcg
      361 gggtgcgggg cggaaccccg accaagtggg ccaaatcact gagtgtctcc cgcggtggtg
      421 accgccgctt tggtgcgcca actacgtggg ccgcacttga gtacgtgtcc gtgatgcaga
      481 atgcgatcga gcatggaggt gaccgcggcg acatcgccca gtagctttcc ccagtcttcg
      541 actgggcggt tcgatgtcaa cagtgtactc acgcgttcgt agcgacgcat gatgatctcc
      601 agcaggtcct cggccgccgt cagcggcagc ttgcgcatac cgaagtcatc gatgatgagc
      661 agcggcacag tcgccaccga ttccatgaac gccttacgcg tgccgtcgac cacagcctcg
      721 gccagttcgt ctagcagcac gtgcgtttcc cgatagagca cacggtaatt ctgttgtatg
      781 gcggcttgtc caatggcctg cgccaagtgg ctcttgcccg tgccgcccgg gcctaaaaac
      841 aacccgtctt cccgtttgcc gatgaatgcg cacgtggcca gatcgaacac caggctgcga
      901 ttcatcttcg ggttgaacgt aaagtcgaag ttgtccagcg tcttgtgtgg gtcacggaat
      961 gcggcctgct tgcggcgtcg atcgagcagt cgttcgctgc gccggctcag ctcgtcgctg
     1021 accagcgacg agatcagatc gatcggcgcc atggcttcgg cttgcgcctg gcgcagtcgg
     1081 gtttccagca cggcggcgat gccgccgagc cgtagttgtc gtaacgcacg ttctaattca
     1141 atctggttca tagttcgctc tccttggttt tgttctcgat gaagtcgcgg tacaacgtga
     1201 gctgacggat cagcggatcg acctggcgca agctcagcgg catctgcggg tgccgctcca
     1261 ggtagcggcg cacaaaacgg taatcgcaga cccccagttc cagggcggcg gcacaggcct
     1321 cctcgacgct gggggcgccg tacttcttgg ccagggacaa cacgccctga atccggcgca
     1381 ccatggtttc tccgtgctcg ccgtgcatcc cgcggcaaag cgctccgatc tgcgatccgg
     1441 ccttgtcggc gcggcacagc agggcgagcg tgcccagcgg cgtatgtttg gggcggtctt
     1501 catccttgat ccgatggccg ccgcgcacct ggcgcaagtg ctcgcgtagt aactggcccg
     1561 tctccggatt catcagtcgc acctgtcgta gatcccactg cacctggaca tgccggccaa
     1621 tccagcccgg cggcgccccg tagtaggccg cttcgacttc cacgcagccg tccagatgca
     1681 cggtgcgctc gccgtactgg tagtagcgga agggctcgac cggcagtggt agcaggagag
     1741 gcttctcctc ggcgaacatg accgcgacct gccgcttggt ggtgccgtga atgcgggtgt
     1801 ccgcccagcg tgcttcccaa tgatccaggt aggtttgggc atcctccagg ctctcgaagc
     1861 ggaggccctt caacggtgtt ttctgcgcat ggccgacacc gctctccacc ttcccctttc
     1921 ggtccgggtc cgccacgcgg catgggaggg ggacggcacc gtaatgccgc agcacatcgc
     1981 ggtagagcgg gttgagcgtg ggatcgtaga tgtcgggcgt aagcacaccc tcgcgcagat
     2041 tatccagcac gaccacgcgc gtcaccccgg tcagccggcg aaaggccttc tcatgcaact
     2101 cggcccagac ctgggaactg gagcggaaca ccagcaagcg aaccgacttg cgactgtagc
     2161 ccaacgtcag tacgaacagc cgcgtccgcc ggtacttgcc gctgtgcggg tcgcggacca
     2221 tggggccggt gccgtagtcg acttgggcct cttcaccggg agcggtctcg atcaccgcgc
     2281 aggcttcccg ccacggactg gggtgaagct tggagacgaa gcgccggacg ctctggtagc
     2341 cggcggcgaa actgcacgta tccaccagat cctgccagat cgccatggcg ttgcggccac
     2401 gggagagtcc cagttcgatt gcttcccggt agggccagca ggcgcttgcc gaggggctgc
     2461 gcccgggcgg tgaatctggc actacggtca agccggcggc caacccagcg ccaaagtcgg
     2521 tggtcacctc attggccggt tttgccagga caggatcggc ggcggccccg ctgacgggtt
     2581 ttcccgccgc agagtcggtg atcacctcgt tggccggttt tgccggtgac tgccgtcccc
     2641 aaccgccagg cggccgaaag gcgaccttgg cagctttcaa ataggcggcc gccgtttccc
     2701 gacgtacgcc ggtgttcttc tggattcgcc gtagcgacca gcccaagcgc cccagagcga
     2761 tgacttgctg tttcttttct tcgttcaaga cgttactcac atcgccgagg gtatcgccct
     2821 cgggtcggaa tcaacgtcct ggactgcggt ttatcgttgg ccggttttca ggtgatcatc
     2881 aatggccggt tttgggtgat caccgagggc ctttctttcg gcatcgctgc cgatgaaacc
     2941 gataagagtg attcgattga ggtacatatg tttctcctgt tctgcccgtt tgtctcgggc
     3001 accggaaagc gagtgggcgc ggctcccaag acaagaagct gcttttctga ggggaccgga
     3061 acgggggagc cagaaccgga agggtgcgag agcaaagcag aagcttcgca gacgccgggc
     3121 gccgcaaaag ccccgaagca gtgccgagac aacgagcagg ataggaaacg cgaccgaacg
     3181 gatcaccact ttgctggtcg ccgtcgctgg agg
//
LOCUS       DASQOP010000106         5487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2257006, whole genome shotgun sequence.
ACCESSION   DASQOP010000106 DASQOP010000000
VERSION     DASQOP010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5487
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2257006"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..1001
                     /locus_tag="VE030_04305"
     CDS             225..1001
                     /locus_tag="VE030_04305"
                     /inference="COORDINATES: protein motif:HMM:NF021465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HZF22674.1"
                     /translation="MGIGSSIRASPGGSRALVLACLAVLTLASWAYLLYQDWAMAHMD
                     IVDMAMPSTAAWGVADLTLVFMMWAIMMTGMMLPSAMPMVFAVEPISRRRGTAPFGAS
                     LLFTGGYLSVWMAFSVLATLVQWSLHSAALMSADMKASTSLLAGLLLAAAGLYQLSTV
                     KLACLTRCRSPLDFLLTEWRDGRWGTFVMGLRHGAFCTGCCWGLMALLFALGVMNLAW
                     VAVISVVVLLEKVTPRGVWLSRGSGVLLIGWGAYVLLGGT"
     gene            1246..1473
                     /gene="rpmE"
                     /locus_tag="VE030_04310"
     CDS             1246..1473
                     /gene="rpmE"
                     /locus_tag="VE030_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HZF22675.1"
                     /translation="MKPEIHPEYQEITVTCSCGNTFKTRSTLGKPLHVEVCSECHPFY
                     TGKQKIVDTAGRVEKFRQKYTKKTAAKTPAA"
     gene            1612..2496
                     /locus_tag="VE030_04315"
     CDS             1612..2496
                     /locus_tag="VE030_04315"
                     /inference="COORDINATES: protein motif:HMM:NF022457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo hydroxylase family protein"
                     /protein_id="HZF22676.1"
                     /translation="MESIILELLSPTNWDAFIDSIEATNAMQALEQGRVLLFRALRFP
                     LSPDEQRLLSPECADPKSKNVSYDFGKDEIRGTRLQDGARQDLTAMMRRFATRARDFA
                     SRLLPAYAKHLEMGRTSYRPVEIRGRPSSGTKDDSRLHVDAFASSPVQGRRILRLFAN
                     VNPLQQVRVWQLGEPFEQFAPRLLPQIRLPWPGSAWLLRQLRVTKRRRTPYDHVMLGL
                     HDRGKRDSNYQRTAPREEVNFPAGAVWMFFSDRVLHAALSGQYALEQTFYLPVEAMAD
                     QRDSPLRILENYYGRALV"
     gene            2600..4261
                     /locus_tag="VE030_04320"
     CDS             2600..4261
                     /locus_tag="VE030_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22677.1"
                     /translation="MLRPAHLLRNEKLASLLAENPLALVLLICLAWTLLGLVGHDPWK
                     PNEAQTFGVVYHLMQSGDWVVPMLAGEPFMEKPPLIYLTAALFGALFSPVLSLHDAAR
                     LATGAYMGLTFIFVALAAKELYGGNKSWMATLMLLGCGGLLIRGHQLISDVALLAGLA
                     MGLYALALSARRPLLAGFWLGTGLGVMFFSTGLVEPLMLLATMVLLPVVSPHWRTRAY
                     ALTALVALVVAAPWALIWPSALYSRSPELFAQWFWTENMLRLKGLFSFNDEDEYFYYL
                     KALPWFAWPALPFALWTLWTQRWVGLRKPGILLPLAAFAAFFAFLSLIGEARDVLGLP
                     LLLPLSMLAAARFETLRRGAANAYYWFAIMLFTFFLLVGWFYWVAIDFGVPARLGKHM
                     MEMQPEYQAHTHPLAILLTFIFTAAWFVLLFNVRRSPERPVIIWAAGMTIVWTLVALL
                     LVNYIDTGKTYRSMVMRLSQALPPAHGCIYSQSLGEPQRAMLHYFANIITVRLEKSGP
                     RPACDLLITQDHWKDPGQAGGPWEMIWEGRRPGDKVERYRLYRQR"
     gene            4258..5127
                     /gene="hslO"
                     /locus_tag="VE030_04325"
     CDS             4258..5127
                     /gene="hslO"
                     /locus_tag="VE030_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HZF22678.1"
                     /translation="MSDSLQRFLLESTPVRGEIVHLDATWRAVLERRRYPALLQALLG
                     DMMAAAALLSAILKFEGSLIMQMQGNGPVKLLVVEATSEHTLRATAKWEGELVQGSVT
                     ELLGAGRFVISIVPSSGKQTYQSIVAIEGETIAQALEHYMAKSEQLETRLWLASDSRQ
                     AAGMLLQKLPAAPTQDADAWNRATQLGETIRREELLSLPAREIIRRLYHEEDVRVFDS
                     RPVAFRCSCSRERVTSMLRMLGHNEVKSIIAERNTVEVDCEFCGRHYTFDAVDAEQVF
                     AADFITRAQPTKH"
     gene            5324..>5487
                     /locus_tag="VE030_04330"
     CDS             5324..>5487
                     /locus_tag="VE030_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HZF22679.1"
                     /translation="MPDDERLRIDKWLWAARFYKTRALAAAAVEGGKAQVNGERVKPA
                     KLLKAGDALIV"
BASE COUNT          979 a         1715 c         1697 g         1096 t
ORIGIN      
        1 ccagtcaatc ccgatacttg cacggggttt gaacactcac ctgattattg catttggctt
       61 tccctggcgt ccctggcggt caacgcttgg ccgtaagggt ttggcgatga gtgtttccgc
      121 caaccgatca atttttcaat ccccgggcgc cgtataaccc acacgaatgt ccgaacgacc
      181 tatttttagt atcccgcggt cgatcccgtc ccgacccgat tttgatggga atcggttcct
      241 ccattcgcgc ctcgccgggc ggtagccggg cgctggtgtt ggcctgcctt gccgtcctga
      301 cgctggcttc ctgggcgtat ctgctctatc aggactgggc catggcacac atggacatcg
      361 tcgacatggc catgccgagt acggccgcct ggggtgttgc ggatctgacg ctggtgttca
      421 tgatgtgggc gatcatgatg accggaatga tgcttccgtc cgcgatgccg atggttttcg
      481 ctgtcgagcc gatcagtcgc cggcgtggaa cggcgccatt cggcgcaagc ctcctcttca
      541 ccggcggtta tctcagtgtg tggatggcat tcagcgtcct cgccacgctc gtccagtgga
      601 gcctgcattc cgccgcattg atgtcggccg acatgaaagc ctccacctcc ctgctcgccg
      661 gcctgctcct cgcagcagcg ggactgtatc aactgagcac ggtgaagctg gcgtgcctga
      721 cgcgctgccg gtcgccgctc gattttctgc tcaccgagtg gcgcgacggc cggtggggta
      781 cattcgtgat gggactgcgt catggtgcct tttgcaccgg gtgctgttgg gggctgatgg
      841 ccttgctgtt tgcgctcggg gttatgaacc tggcgtgggt agcggtgatt tccgtggtgg
      901 tgctgctgga gaaggtaacg ccgcgaggtg tctggctctc gcgcggttca ggcgtgctcc
      961 tgatcggctg gggggcgtat gtactgctcg gtggcacctg aagacagcgc caccctacga
     1021 tgcggaccga ggactggaga ctgatccttc tctcgcaacc cgtctccccg gttgccacag
     1081 ggcatggcgc aatcatgtgt tcatgacgag ttccgggtcc ggatttccta agcccttgaa
     1141 actccggccg ggaccctcat gtagggttgc caaaaaccga ttcgacaggg ataatccgcc
     1201 ccctttggaa gcgcaaatcc cgcttccgcc agaaaggacc cgaaaatgaa gcccgagatc
     1261 cacccggaat accaggaaat taccgtcacc tgcagttgcg ggaatacttt caaaacccgc
     1321 tccaccctgg gcaaacccct gcacgtggaa gtctgctcgg aatgccaccc gttctatacc
     1381 ggcaagcaga agatcgtgga caccgccggc cgcgtggaga aattccgcca gaagtacacc
     1441 aagaagaccg ccgccaagac gccggccgcc tgagcccagc gtccggccgg cacccaaaaa
     1501 aggcagcgaa agctgccttt ttctttcttc accggtcgcc ggccggccga tagaatgtaa
     1561 ggaaccgggc tcaaacccgg cacctctacc ggacatcgaa gagaaaccgc gatggaatcg
     1621 atcatcctgg agttgctgtc tcccacgaat tgggacgcgt tcatcgacag catcgaggcc
     1681 acgaacgcga tgcaggcgct ggaacaaggc cgcgtcctgt tgttcagggc gcttcgattc
     1741 ccgctgagtc cggatgagca acgcttgcta agtccggaat gcgccgaccc aaagtccaag
     1801 aacgtcagct atgacttcgg caaggacgag attcgcggaa cccgtctgca agacggggcg
     1861 cgacaggacc tgactgcgat gatgcgcagg ttcgccaccc gggcgcggga tttcgccagc
     1921 aggcttctgc ccgcttacgc gaaacatctc gaaatgggac gcacaagcta ccggccggtc
     1981 gagattcgcg gacggccgtc ctcgggcacc aaggacgatt cgcgcctgca cgtggatgcc
     2041 tttgcttcca gccccgtcca gggccggcgt attctgcggc tgttcgccaa cgtcaatccg
     2101 ctgcagcaag tgcgtgtatg gcagctcggc gagccattcg aacaattcgc gccccgcctg
     2161 ctgccgcaaa tcaggctgcc gtggcccggc tccgcctggc tcctccggca actacgtgtc
     2221 accaagaggc gccgcacccc ttacgatcac gtcatgctcg gcctccatga ccgcggaaag
     2281 cgagactcca attatcagcg caccgcgccg cgggaggagg taaatttccc ggcgggcgcg
     2341 gtctggatgt ttttttccga tcgcgtgctg cacgcggcac ttagcggcca gtacgcgctg
     2401 gaacagacgt tttacctgcc ggtggaagca atggccgatc aacgagactc gccgctgcgt
     2461 attttggaga actactacgg gcgagccctt gtctgagaag cagccgctga gccttacagg
     2521 cagccacacc gctgaccacg cagcgacgtc ctaagatcaa aagcacaacg acggacacga
     2581 cgacttctct ccaccgctca tgcttcgccc cgcgcatctg cttcgtaacg agaagctcgc
     2641 ctcgttgctc gccgaaaatc cgctagcgct cgtccttctg atctgcctgg cgtggacgct
     2701 gcttggcctg gtgggtcatg acccctggaa gccgaacgag gcccaaacct tcggcgtggt
     2761 ctatcacctg atgcaaagcg gcgactgggt agtgccgatg ctggcgggcg agcccttcat
     2821 ggagaagccg ccgctgatct acctgacggc ggcgttgttc ggtgcgctgt tctcgccggt
     2881 cctgtcgctg cacgacgccg cccgcctcgc gaccggcgca tacatgggcc tcactttcat
     2941 tttcgtcgcg ctggcggcaa aagaactcta cggcggcaac aagagctgga tggccacgct
     3001 catgctgctc ggctgcggcg gcctgctgat acgcgggcat cagttgatca gcgacgtcgc
     3061 gctgcttgcg gggctggcga tgggcctgta cgcgctcgcc ctgtcggcgc ggcggcccct
     3121 gcttgccgga ttctggctgg gtaccggcct gggcgtcatg ttcttctcca ccggactggt
     3181 cgaacctctg atgctgctcg cgaccatggt gttgctgcct gtggtttcac cgcactggcg
     3241 cacgcgcgct tacgcactca ccgcgctggt tgccctggtg gtagcggctc catgggcgtt
     3301 gatctggccg agcgctttgt attcgcgctc gccggagttg ttcgcgcagt ggttctggac
     3361 cgaaaacatg ttgcgcctga aaggcctgtt ttcgttcaac gacgaagatg aatacttcta
     3421 ctacctgaag gcgctgccct ggttcgcctg gccggcgctg ccgttcgcgt tgtggacgct
     3481 gtggacgcag cggtgggtgg gactgcgcaa acccgggatc ttgctgccgc tggccgcctt
     3541 cgctgcgttc ttcgccttcc tcagcctcat cggcgaggcc cgtgacgtgc tcggcctgcc
     3601 gttgctgctg ccgttgtcga tgctcgccgc cgcccgcttc gagacactgc ggcgcggcgc
     3661 ggccaacgcg tattactggt tcgcaatcat gctgttcacg ttcttcctgc tggtgggctg
     3721 gttttactgg gtggcaattg atttcggcgt tccggcgcgg ctgggcaagc acatgatgga
     3781 gatgcagccg gagtaccagg cgcataccca cccgctggcg attctgctca cgttcatttt
     3841 caccgccgca tggttcgtgc ttttgttcaa cgtcaggcgc agccccgagc ggccggtgat
     3901 catctgggcc gcgggcatga ccatcgtatg gacgctggtg gcgctgctgc tggtgaatta
     3961 catcgacacc ggcaagacct accgctcgat ggtcatgcga ctctcgcagg cgctgccccc
     4021 cgcacatggc tgcatctaca gccagtcgct aggcgagccg cagcgcgcga tgctgcatta
     4081 ttttgcgaat attattaccg tgcggctgga aaaatccgga ccgcgccccg cctgcgacct
     4141 gctgatcacc caggaccact ggaaagatcc cggccaggcc ggcggaccct gggaaatgat
     4201 ctgggaaggt cggcgccccg gcgacaaggt cgaacgctac cggctctata gacagcgatg
     4261 agcgattccc tgcaaaggtt cttgctggag agcacgccgg tgcgcggcga gatcgtgcac
     4321 ctggatgcca cctggcgcgc ggtgctcgag cgccgccgct atccggccct attgcaagcg
     4381 ctgctgggcg acatgatggc ggcggccgcg ctgctctcgg ccattctcaa gttcgaaggc
     4441 tcgctgatca tgcagatgca gggcaacgga ccggtaaagt tgttggtggt cgaggcgacc
     4501 agcgagcata cgctgcgcgc caccgccaag tgggagggcg aactggtcca gggcagtgtg
     4561 accgagctgc tcggcgcggg gcgtttcgtg atctcgatcg tgcccagcag cggcaagcag
     4621 acctatcaga gcatcgttgc catcgagggc gaaaccatcg cccaggcgct ggagcattac
     4681 atggccaagt ccgagcaact ggagaccagg ctgtggctcg cctccgattc ccggcaggcc
     4741 gccggcatgc tgctgcagaa gctgcccgcc gcaccgaccc aggatgcgga cgcgtggaac
     4801 cgcgcgacgc aactgggcga aaccatcagg cgcgaagaac tgctgtcgct gccggcccgc
     4861 gaaatcatcc gccgccttta ccacgaggaa gacgtgcgcg tgttcgactc caggcccgtg
     4921 gcctttcgct gctcgtgctc gcgcgaacgc gtgacctcga tgctgcgaat gctcggccac
     4981 aacgaagtga agtcgatcat agcggaacgc aacaccgtcg aagtggattg cgaattctgc
     5041 gggcggcact acactttcga cgccgtggac gccgagcagg tgttcgcggc cgacttcatt
     5101 actcgcgccc agccgacgaa gcactagacc tgaatcgcaa aggcgcaaag acgcgaagaa
     5161 gaccgcgcaa aggagagcag gaaatatgtt ctccgtgata ctgaatcgtg gcgatcaggc
     5221 atgagaccga tctttatctc atttctcatc gtcaatttgt ccttcttagc gtttccttcg
     5281 cgtctttgcg tctttgcggt gaaggtctgg atagaggttt tgcatgccgg acgatgagcg
     5341 actgcgaata gacaaatggc tatgggcggc ccgcttctac aagacccgcg cgctggcagc
     5401 agcggcggtc gagggcggga aggcgcaggt gaacggtgaa cgggtcaagc cggcgaagct
     5461 gctcaaggcc ggagacgcgc tgatcgt
//
LOCUS       DASQOP010000107         2248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_446848, whole genome shotgun sequence.
ACCESSION   DASQOP010000107 DASQOP010000000
VERSION     DASQOP010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446848"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..1251)
                     /locus_tag="VE030_04335"
     CDS             complement(223..1251)
                     /locus_tag="VE030_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22680.1"
                     /translation="MAAALTLAAQTADAEDVQVPPQVRGCPAGQAIQGVDFAAHTLIC
                     IPVGASAEIAALTARVTALESAVANERNARLAADNALQASISSEAAARAAADTTLQSN
                     LNAEAITRIAEDGATLASAKAYTDTKVAAGGGGGALSFLQPYISLDVNPINGVNGPHI
                     IFSGANVHVRSGSGSTHDNNTLTGLGNLIVGYDEQQTEAVSRTGSHNLIVGGQHSFTK
                     HGGLAAGAANTLDGVSAFAAGVRNVASGNFSSVTGGTGNQALASADTVSGGANNVASG
                     GSSSISGGQENQTVAVASSVSGGHQNTANGLYASVTGGFTNTAAGEASSVAGGQGNVA
                     GGLFQHVP"
     gene            complement(1487..>2248)
                     /locus_tag="VE030_04340"
     CDS             complement(1487..>2248)
                     /locus_tag="VE030_04340"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HZF22681.1"
                     /translation="MGEHCELMAKEWGIGRAEQDELALASHRKAAEAWRGGFYSELVF
                     AFRNLKTDNNIRADASIEKLASLKPAFDRSGSGTLTAGNSTPLTDGAACVLLGSEEWA
                     RSRGIPIQAYLTHTASAAVDYAGLAGPREGLLMAPTYAVPQMLDRAGIRLQDFDIYEI
                     HEAFAAQVLCTLKAWESEEYCRTRLKRTQPLGAINRAKMNPKGGSVAVGHPFGATGAR
                     IVAGLAKQLADRGSGRGLISICTAGGMGVAAILER"
BASE COUNT          377 a          718 c          722 g          431 t
ORIGIN      
        1 gaaaaataca tcgcggactg cgcccgaacg ctcctcgcgc gcaccgacgc cgccagtcgg
       61 ccggtgcggc tgctcggcgt gggcgcgcac ggcctcgagc gcgagggcga cggccatccg
      121 gaatccgagc ggctattttg aaaaatgggg acagacccca ttttccgttg ctcagcgcag
      181 cccgcaggaa aatggggtct gtccccattt ttctcttgag aattacggca cgtgctggaa
      241 cagcccacca gcgacgttgc cctgtcctcc ggcgacgctt gaagcttcac ccgcggcggt
      301 attggtaaag ccgccggtga cactggcgta gagcccgttt gccgtattct ggtggccgcc
      361 gctgacgctg gaagcaactg ctaccgtctg gttttcctgc ccgccgctga tgctcgagga
      421 gcccccgctg gcgacattgt tggcgccgcc cgagacagta tcggcacttg ccaatgcttg
      481 gttgcctgtg ccgcccgtca cgctggagaa gttaccactc gctacgtttc tgacaccggc
      541 tgcaaatgct gaaaccccgt cgagcgtgtt ggctgcgccg gcggccaggc cgccatgctt
      601 ggtgaagctg tgctgcccgc ctacgatgag gttatgcgat ccggtgcggc ttacagcctc
      661 ggtctgctgc tcgtcgtacc cgacaatcag attgcccagg ccggtaagcg tgttgttgtc
      721 gtgggtgctg ccgctgccgc tacggacgtg aacattggcc ccgctgaaga taatgtgcgg
      781 cccgttgacg ccgttaatgg gattcacgtc gaggctgata tagggctgca agaagctcag
      841 cgctccgccg ccaccgcctg ccgcgacttt tgtatcggtg taagccttcg cgcttgcaag
      901 agtggcgcca tcctctgcaa tccgcgtaat cgcctcggca tttagattgc tttggagcgt
      961 cgtgtcggca gcagcacgag ccgccgcttc cgagcttatg ctggcttgca aagcattgtc
     1021 cgcggctaat ctcgcattgc gctcattggc aaccgcgctt tccaatgcgg tcacgcgcgc
     1081 agtgagtgca gcgatctctg ccgaagcgcc tactggaatg catatcaggg tgtgtgcggc
     1141 aaagtccacg ccctgaattg cctgccctgc cggacaacca cgaacctgag gtggaacctg
     1201 aacgtcctca gcgtcggccg tttgtgccgc caacgtcaat gctgctgcca ccagacatga
     1261 tctgatccca tgcatgttca cagcgacccc cgtagataaa tgccactgaa caagtggctg
     1321 aacagccgcg aagttatttg gcagagtggt acagaggtac cgaaaggaca ggccctgctg
     1381 gcccgcaaag aaagcgaaaa cgggaagctt tcactttgtt gactactgga cggaaacctt
     1441 actcttgcgc ctgcggaccc gcaatgatcc aaacgggggg gtggtgtcag cgctcgagga
     1501 tggctgcgac ccccatcccg ccggctgtgc agatcgagat gagcccccgg cccgagccgc
     1561 ggtcggcgag ctgcttcgcg agcccggcga cgatgcgcgc gccggtggcg ccgaacggat
     1621 ggccgaccgc gacgctgccg cccttcgggt tcatcttcgc gcggttgatc gcgcccaaag
     1681 gctgcgtacg tttgaggcga gtgcggcagt actcctccga ttcccaggcc ttcagcgtgc
     1741 agagcacctg cgcggcgaag gcttcgtgga tctcgtagat gtcgaagtcc tgcagccgga
     1801 tgccggcgcg atccagcatc tgcggcaccg cgtaggtcgg ggccatcagc aggccttcgc
     1861 gcgggccggc gaggccggcg tagtcgacgg cggccgacgc ggtgtgcgtg aggtaagcct
     1921 ggatcgggat gccgcgcgag cgcgcccact cctccgagcc gagcagcacg cacgcggcgc
     1981 cgtcggtgag cggcgtgctg ttgccggcgg tgagcgtgcc ggagccgctg cggtcgaacg
     2041 cgggcttcag cgaggcgagc ttctcgatgc tcgcatcggc gcggatgttg ttgtcggttt
     2101 tgaggttgcg gaaggcaaac acgagctcgg agtagaagcc gccgcgccac gcctccgcgg
     2161 ccttcctgtg gctggcgagc gcgagctcgt cctgctccgc gcggccgatt ccccattcct
     2221 tcgccatcag ctcgcagtgc tcgcccat
//
LOCUS       DASQOP010000108         4220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1454613, whole genome shotgun sequence.
ACCESSION   DASQOP010000108 DASQOP010000000
VERSION     DASQOP010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1454613"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1894
                     /gene="pbpC"
                     /locus_tag="VE030_04345"
     CDS             <1..1894
                     /gene="pbpC"
                     /locus_tag="VE030_04345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HZF22682.1"
                     /translation="EDKRFYRHSGVDWLALSDAALDTLLFSQPRGASTISMQVAAHLD
                     DALRPGSQKRTVSQKWDQIKAAKELEKAWSKSQVLEAYLNLSTFRGELQGIAAASRGL
                     FGKSPDGLDERESLLLAVLLRGPNSKPETAAKRACALAGRMKIGLACPQLEAQAQAAL
                     QGVPTIAPRASLAPHVARELLSAQRARVQTTLDAELQAFAAEVLQQQLVALAEQHVAD
                     GAVLVTDNRTGEVLAYVGNAGNSASAFYVDGIRAARQAGSTLKPFLYQMAIEDRLITS
                     ASLIDDSPVNLITPTGLYVPQNYDRDFKGLTSVRTALASSLNVPAVRTLMLVGADAFT
                     ERLKMLGFDDVTEDGDFYGYSLALGSAEVSLWQLTNAYRTLANGGRWSPLKLQRATRT
                     AATPLLDPAAAYLIADVLADPLARSVTFGLESPLSSRHWAAVKTGTSKDMRDNWCIGF
                     TDRYTIGVWVGNFDGSAMRDVSGVSGAAPVWLEMVNYLHRRQASTAPRAPAGVVRTQV
                     RYDQDVEAERMEAFLAGTETARIAAKAAAAIRAGIAYPGNGTIIALDPDIPESVQRVR
                     FMVSPQVAGYRWHLDDEEIGVSDQPIFWVPRPGSHELSIVDQSGTAVDRVRFEVRSSI
                     RQSQVQ"
     gene            2152..3528
                     /locus_tag="VE030_04350"
     CDS             2152..3528
                     /locus_tag="VE030_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZF22683.1"
                     /translation="MATTFSPVFEKATYDKVAWRLIPFLFLCYIVAFLDRVNVGFAKL
                     QMAPDLKFSDTIYGFGAGIFFIGYFIFEVPSNVILERVGARIWIARIMITWGIISSAF
                     MFTNDIHWGVIATMFNCTDAEFTFYFLRFLLGVAEAGFFPGIILYLTYWFPASRRAKM
                     VALFMTAIAISNAIGSPISGAIMQYVDGANGWRGWQWLFLLEGIPSVIIGALVFVLLD
                     NGPKTSKWLTDAERNVLAHRLEEDDVGKQELGRRHSFLDAFRDGRVWALAVVYFCGVV
                     CFYAVNFWMPTIIQEVGIAKTDYLKVGLLSMIPWGLAAVAMVIWGAHSDRTGERRWHA
                     SGGLLLAIGGMIGLALVGKGNVVVSLTALSMVTVGILSWVATFWSLPTSFLSGTAAAA
                     GIAWINSVGNLGGHFGPDLIGRIRTATGGSSEAAFFTLAAVAFVGVVIILLLPRTQKA
                     VAASAIAK"
     gene            3875..>4220
                     /gene="rpsF"
                     /locus_tag="VE030_04355"
     CDS             3875..>4220
                     /gene="rpsF"
                     /locus_tag="VE030_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HZF22684.1"
                     /translation="MRHYEIVFIVHPDQSEQVPGMIERYRQMVTSRAGRIHRLEDWGR
                     RQLAFPIQKVHKAHYVLMNIECDQETLTELEHAYKFNDAVVRHLIVNMTRAVTEPSPM
                     MKEEKSRTVVGES"
BASE COUNT          766 a         1304 c         1341 g          809 t
ORIGIN      
        1 cgaggacaag cgcttctacc ggcacagtgg cgtggactgg ctggcgttgt cggatgccgc
       61 actcgatacg ctgctgtttt cgcagccgcg cggcgcctcg accatatcca tgcaggtcgc
      121 cgcgcacctg gacgatgcgc tgcgcccagg cagccagaag cgcacggtgt cgcagaagtg
      181 ggaccagatc aaggcggcga aggagttgga aaaggcctgg agcaaaagcc aggtcctcga
      241 ggcttatctg aatctctcga ccttccgcgg cgaattgcag gggatcgcgg cggcatcgcg
      301 ggggttgttc ggcaagagcc cggacgggct cgacgagcgc gagtcgctgt tgctcgcagt
      361 gctgttgcgt ggcccaaaca gcaagccgga aacggcggcg aaacgcgcct gcgcgcttgc
      421 cggacgcatg aagatcggcc tcgcctgccc gcagctcgag gcgcaggcac aggcggcgtt
      481 gcaaggcgtg ccgaccatcg ctccgcgcgc ctcacttgct ccgcacgtcg cgcgcgaact
      541 tctttccgcg cagcgcgcgc gggttcagac cacactggac gcagagctgc aggcctttgc
      601 ggcggaagta ctgcagcagc aactcgttgc gctggccgag cagcacgtcg ccgacggtgc
      661 cgtgctggtg acggacaaca ggacgggaga agtactggcc tacgtcggca atgccggaaa
      721 tagcgccagc gcgttctacg tagacggcat acgtgccgcg cgccaggctg gttcgacgct
      781 caagcccttc ctttatcaga tggcgatcga ggacagactg atcaccagcg cctccctcat
      841 cgacgactcg ccggtgaacc tcataacgcc gaccgggctg tacgtgccgc agaattacga
      901 ccgcgacttc aaggggctta ccagcgtgcg caccgcgctg gcgtcttcgc tcaacgtgcc
      961 ggcggtgcgt actctcatgc tggtcggcgc ggacgcattc acggagcgct tgaagatgct
     1021 cggtttcgac gatgtcaccg aggacgggga cttctacggt tactcgctgg cgctgggttc
     1081 ggcggaggtc agcctgtggc agctcaccaa cgcctaccgg acgctcgcca acggcggacg
     1141 ctggtcgccg ctcaagctgc agcgcgcaac caggactgcc gcgacaccgc tgcttgatcc
     1201 tgccgcggcc tatctcattg ccgacgtgct cgccgatccg ctcgcacgca gcgtcacttt
     1261 cggcctggaa agtccgttgt cgtcgcgtca ctgggcggcg gtgaagaccg gcaccagcaa
     1321 ggacatgcgc gacaactggt gcatcggctt caccgatcgt tacacgatag gcgtgtgggt
     1381 cggcaacttc gacggcagcg ccatgcgcga cgtttccggc gtgtccgggg ccgcgccggt
     1441 atggctggag atggtgaact acctgcatcg ccggcaggcc tcgacagcgc cccgtgcacc
     1501 agccggtgtg gtgcgcacgc aggtccgcta cgatcaggac gtcgaggccg agcgcatgga
     1561 ggcgtttctg gctggtaccg aaaccgcgcg tatcgccgcc aaagccgccg ccgcaatccg
     1621 tgccggcatc gcctatccgg gcaacggcac catcatcgcc ctcgatccgg acattccgga
     1681 atcggtgcag cgtgtgcgct tcatggtctc gccgcaagta gccggctatc gctggcacct
     1741 ggatgacgag gagatcggcg tgtccgatca gccgatattc tgggtgccgc ggcccggaag
     1801 ccacgaactg tcgatcgtgg accaaagcgg cacagcagtg gatcgggtgc gcttcgaagt
     1861 gcgcagcagc atccgccagt cgcaggtgca gtaggaattc gcggccgcat gtcacgaaca
     1921 gtcaccccgc ttttaacccc ggatcccggc ctgatcgagc ccctgccgca gcaaatctga
     1981 cgctcccaga tgtcgtgcgc ccacagtgcc ttgcgggctg ttagaatacg ccgccaaaat
     2041 caataacaaa cagggtaggg aagccccgat cgagttgcgc aacgcgcgtc gcggtactcg
     2101 atcggggttt cccccgctac acaaacaata atcattgagg aggagactca attggccact
     2161 acattttccc cggtgttcga gaaggcgacc tatgacaagg ttgcatggcg cctgattccg
     2221 ttcctgttcc tgtgctacat cgttgcgttc ctggaccgcg tgaacgtcgg cttcgcaaaa
     2281 ctgcagatgg cgcccgacct gaagttcagc gacacgatct acggctttgg cgccggcatc
     2341 tttttcatcg gctacttcat cttcgaggtg ccgagcaacg tgatcctgga gcgcgttgga
     2401 gcccggatat ggatcgcgcg catcatgatc acctggggga tcatttcttc ggcgttcatg
     2461 ttcaccaatg acattcattg gggcgtgatc gcgacgatgt tcaattgcac cgacgcggaa
     2521 ttcactttct atttcctgcg cttcctgctc ggcgtggccg aggccggttt cttccccggc
     2581 atcatcctgt atctgaccta ctggttcccg gcgtcgcggc gcgcgaagat ggtggcgctg
     2641 ttcatgacgg cgatcgcgat ctccaacgca atcggctcgc cgatttcggg cgcgatcatg
     2701 cagtatgtcg acggagccaa cggctggcgc ggctggcagt ggctgttcct gctcgaaggc
     2761 attccgtcgg tgatcatcgg ggcgctggtg ttcgtgctgc tcgacaatgg ccccaagacc
     2821 tcgaaatggc tcaccgatgc ggagaggaac gtgctcgcgc atcggcttga ggaagacgat
     2881 gtcggcaagc aggagctcgg caggcgccac agtttcctgg acgcgttccg cgacggccgg
     2941 gtctgggcgc tggcggtggt gtatttctgc ggcgtggtgt gtttctatgc ggtgaacttc
     3001 tggatgccca ccatcatcca ggaagtgggc atcgccaaga ccgattacct caaggtcggt
     3061 ctgctgagca tgattccctg gggccttgcc gcggtggcga tggtgatctg gggtgcccat
     3121 tccgatcgca ccggcgaacg gcgctggcat gcatcggggg ggcttttgct cgccataggt
     3181 ggaatgatcg gcctcgcgct ggtcggcaag ggcaatgtgg tggtttccct gacggcgttg
     3241 agcatggtca cggtcggcat cctgtcctgg gtggcgacgt tctggtcgct gccgacgagc
     3301 ttcctctcgg gtacggcggc agccgctggc attgcctgga tcaactcggt cggcaacctt
     3361 ggcgggcact tcggccccga cctgatcgga cgcatccgca cggcgaccgg gggatcgagc
     3421 gaagctgcct tcttcaccct ggctgcggtt gcgttcgtcg gcgtagtgat cattctgctt
     3481 ttgcctcgga cccagaaggc agtggcggct tctgccatcg ccaagtagaa caggggctcg
     3541 cgccaaccgg cagataattt gctgcaaaaa ctagaaaagc ccgccgcttg gcgggctttt
     3601 gttctcttgc acaatgcgtg tgccgggcat catcgagcaa ggcgttcggc cagagcgagt
     3661 gacccacgtg tgctcgccgg tccggcgcag cggacggcca tgcaagcgcc ttgtaacctc
     3721 gcagtaaacc ggattcctgg taaagcgttt gccaaaaggt gattgatctt caaaccgaaa
     3781 tcggtataat tccggccttc cttgcttcac cgggtccgca tcccgggagg ctttccgccc
     3841 gatccgtcgg gcaaacccac aaggagatat attgatgcgg cattacgaga tcgttttcat
     3901 cgtccatccc gaccagagcg agcaggtgcc cgggatgatc gagcgctacc ggcagatggt
     3961 cacttcccgc gccgggcgca tccatcgcct tgaagactgg ggacgcaggc aactcgcctt
     4021 cccgatccag aaagtccaca aggcgcatta cgtgctgatg aacatcgaat gcgatcagga
     4081 aaccctgacc gaactcgagc acgcctacaa gttcaacgac gcggtggtgc gccacctgat
     4141 cgtgaacatg acccgcgccg tgaccgagcc ttcaccgatg atgaaggaag agaagtcgcg
     4201 taccgtggtg ggcgagagcg
//
LOCUS       DASQOP010000109         4860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_447761, whole genome shotgun sequence.
ACCESSION   DASQOP010000109 DASQOP010000000
VERSION     DASQOP010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447761"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VE030_04360"
     CDS             complement(<1..436)
                     /locus_tag="VE030_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22685.1"
                     /translation="MKLLPGSLFGRIALILLGGLVAVQLLTTAIHIAERDDLVVRTVA
                     SRAAARIGDVVRVLNAVTPAQRPRIMDAIADATLKLNDGKPAGGDSAASDEETELHRL
                     AREALALALDPGTRFRVVDAQTVNINPDSWYAREFGERAGMRI"
     gene            complement(433..1149)
                     /locus_tag="VE030_04365"
     CDS             complement(433..1149)
                     /locus_tag="VE030_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZF22686.1"
                     /translation="MRDRILIVDDDEDIRKLLEEYLRKNGFDAQAVADGPAMREALLA
                     RPASLVVLDLMLPGEDGLSLCRQLRARSQVPVLMLTAKGDPVDRIIGLETGADDYLAK
                     PFDPRELLARIRSVLRRAKALPADTEINVPESFSFSGWRLDTRERNLRAPDGVVVPLS
                     GAEYRLLLIFLQNPNAVLSRDQLSNFTFGRDADPLDRTIDMQVSRLRERLRDGAREPA
                     IIKTVRGKGYVLAVRVESQS"
     gene            1309..2631
                     /locus_tag="VE030_04370"
     CDS             1309..2631
                     /locus_tag="VE030_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HZF22687.1"
                     /translation="MSTAPLPPPAAEELIPAGMQRSAGPAAWWQRTWVWLLVLAILGG
                     TAYFVFAKPEAGKPAGMQQSGGKPGMNAMNRSQPVVAAAAKVGDINVYLNGLGTVVPL
                     NTVTVKTRVDGQLSAVMFREGQMVKQGELLAQIDPRPYQVQLAQAEGTMARDQALLKN
                     AQADLDRYRTLFEQDSIAKQQLDTQASLVRQYEGAIKADQAQIDSAKLQLTYSRITAP
                     LSGRLGLRQVDAGNIVHASDTNGIVVITQLQPVTVVYTLPEDNISAVMKKLHAGQKLT
                     VDAYDRAGKTKLASGMLLTVDNQIDPATGTIKLKAQFANDDSSLFPNQFVNARMLLDV
                     MREIILIPSAAVQRGTQGTFVYVVRDDQSVAVRTVKLGPTEGETAAIESGLSAGEIVV
                     VDGTDKLRDGAKVEVGERNAAAAPSDGGTPKQGKRRRDGDASPSASGS"
     gene            2644..>4860
                     /locus_tag="VE030_04375"
     CDS             2644..>4860
                     /locus_tag="VE030_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZF22688.1"
                     /translation="MNPSRPFILRPVATSLMMVAILLSGIVAYRLLPLSALPEVDYPT
                     IQVVTLYPGASPDVTTSSITAPLERQFGQMPGLNQMSSTSSGGASVITLQFSLDLTLD
                     VAEQEVQAAINAATSLLPQDLPSPPIYSKVNPADTPILSLAITSSSLPLPKVQDLIDT
                     RIAQKISQLPGVGLVSLSGGQRPAVRVQANPKALAAYGMSLDDLRTAIGNANVNQAKG
                     SFDGPSRASTIDANDQLKSADEYQRLIIAYKNGAPIRLTDVADVVDDAENVRLAAWAN
                     DKAALILNIQRQPGANVIVVVDRIKKLLPQLQAALPGSVDIVMLTDRTTTIRASVKDV
                     QFELLLAVALVVMVIFLFLRNVAATIIPSVAVPLSLVGTFGVIYLAGFSINNLTLMAL
                     TIATGFVVDDAIVMIENIARYIEEGMKPLEAALKGAEQIGFTIISLTFSLIAVLIPLL
                     FMGDVVGRLFREFAITLAVAILISAFVSLTLTPMMCARLLHHIPKSEQSRFYRSSGRF
                     FDAVIARYGRMLDWVLDRQAATLLVALGTLALTVLLYFFVPKGFFPVQDTGLIQGISE
                     APQSISFAAMAQRQQDLAKIVLQDPAVESLSSFIGVDGNNATLNSGRMLINLKPLKAR
                     GIDATGVIRRLQPQLAQVAGISLYMQPVQDLTIEDRVSRTQYQFSVEDANADNLGIWV
                     PKLVERLRAATQLADVASDLQDRGLQAFVEIDRSTASRLGITPAAIDNALYNAFGQR"
BASE COUNT          908 a         1640 c         1466 g          846 t
ORIGIN      
        1 ggatgcgcat cccggcacgc tcgccgaatt cacgcgcgta ccaggaatcc gggttgatgt
       61 tcaccgtctg cgcgtcgacc acccggaatc gcgtgccggg atcgagcgcc agtgccagcg
      121 cttcgcgcgc caggcggtgg agttcggttt cctcgtccga ggcggcagaa tcgccgcccg
      181 ccggcttacc gtcattcaat ttcagcgttg cgtcggcaat cgcgtccatg attcgtggcc
      241 gctgcgccgg ggtcacagca ttgagcacgc gcacgacgtc gccgatcctg gccgccgccc
      301 gcgatgcaac ggtgcggaca accagatcgt cgcgttcggc gatgtgaatc gcggtagtaa
      361 gcagctggac ggcgacaagg ccgcccaaca ggatcagcgc gatgcggccg aacagcgagc
      421 ccggcagcag cttcaagatt ggctctccac gcgcaccgcc aggacatagc ctttgccgcg
      481 cacggtcttg atgatcgccg gctcgcgcgc cccgtcccgc aggcgttcgc gcaggcggct
      541 cacctgcatg tcgatggtgc ggtcgagcgg gtcggcatcg cgcccgaacg tgaaattgga
      601 aagctggtcg cgtgacagga ccgcgttcgg gttctgcagg aagatcagca gcagccggta
      661 ctcggcgccc gacaagggca ccaccacgcc gtcaggcgcg cgcagattgc gctcccgtgt
      721 atccagccgc cagccggaga aactgaagga ttccggcacg ttgatctcgg tatcggcggg
      781 cagcgccttg gcgcgccgca ggacgctgcg gatgcgcgcc agcagttcgc gcggatcgaa
      841 aggcttggcc agataatcgt cggctcccgt ctccagccca ataattcggt ccaccggatc
      901 gcccttcgca gtgagcatca gcaccggcac ctgcgagcga gcgcgcaact gccggcacag
      961 cgacagaccg tcctcgcccg gcagcatcag gtccagcacc accagactcg ccggcctggc
     1021 cagcagggcc tcgcgcatgg ccggcccgtc cgccactgcc tgcgcatcga acccgttctt
     1081 gcgcaggtat tcctccagca gtttgcggat gtcttcgtcg tcgtcaacaa tgagtatgcg
     1141 atctcgcatg gctgccctta tacctccgac tgaaatcatt tgggagaagt tttgtcattt
     1201 actgtaaccc tcgccagacc ccggacattt cgttacatag cgcaccgggt catgacatac
     1261 agttgccact ccccagaaaa gcattcggaa tcatcccgga accaaccgat gagcaccgcc
     1321 ccccttccgc cgccggcggc tgaagagctg attcccgccg gcatgcaacg cagcgccgga
     1381 cccgctgcct ggtggcagcg cacctgggtc tggttgctgg tgctcgccat cctgggcggc
     1441 accgcctatt tcgttttcgc caaacccgag gccggcaagc cggccggcat gcagcagtcc
     1501 ggcggcaagc caggcatgaa tgcgatgaac cgcagccagc cggtggtcgc agccgcggcc
     1561 aaggttggcg acatcaacgt ttacctgaac ggactcggta cggtcgtgcc gctcaacacc
     1621 gtcacggtaa agacacgtgt cgacggccaa ctcagcgcgg tgatgttccg cgagggccag
     1681 atggtaaagc aaggcgaact gctcgcgcag atcgatccac gtccttacca ggtgcaactg
     1741 gcgcaggccg aaggcaccat ggcccgcgac caggcgttgc tgaaaaacgc ccaggccgac
     1801 ctcgatcgtt atcgcacgct gttcgagcag gactccatcg ccaaacagca actcgatacc
     1861 caggcctcgc tggttcgcca gtacgaaggc gcaatcaaag ccgaccaggc gcagatagac
     1921 agcgccaagc tgcaactcac ctattcccgc atcaccgctc cgctcagcgg acggctcggc
     1981 ctgcgccagg tcgacgccgg caacatcgtc cacgccagcg ataccaacgg catcgtggtc
     2041 atcacgcagc tgcagccggt cacggtggtc tacacccttc ccgaagacaa catctctgcc
     2101 gtaatgaaga aattgcatgc cggtcagaag ctgacggtgg atgcgtacga tcgagcgggc
     2161 aagacgaagc tcgccagcgg aatgctgctc acggtggaca accagatcga tcccgccacc
     2221 ggcacgatca agctgaaagc gcagttcgcc aacgacgatt ccagcctgtt cccgaaccag
     2281 ttcgtgaacg cgcgcatgct gctcgacgtc atgcgcgaaa tcatcctcat cccgtcggct
     2341 gcagtccagc gcggtacgca aggcacgttc gtctacgtag tcagggacga ccagagcgtt
     2401 gccgtgcgca cggtgaagct tgggccgacc gaaggagaga ccgccgccat cgagtcgggg
     2461 ctgtcggccg gagagatcgt ggtggtggac ggtaccgaca agcttcgcga cggcgccaag
     2521 gtggaggtcg gcgagcgcaa tgccgccgcc gcgccttcag acggcgggac gccgaaacaa
     2581 ggcaagcgcc gccgcgatgg cgatgcatcg ccatccgcat ccggcagctg atccgcgcgg
     2641 cgcatgaacc cgtcgcgtcc gtttattctg cgtccggtgg cgacctcgct gatgatggtc
     2701 gccattctgc tttccggcat cgtcgcctat cgcttgctgc ccctgtcggc gctgccggag
     2761 gtcgactatc ccaccatcca ggtcgtgacc ctgtatccgg gcgcgagtcc cgacgtcacc
     2821 acctcttcga tcaccgcgcc gctggagcgc cagttcggcc agatgccggg actgaaccag
     2881 atgtcgtcca cgagttccgg tggcgcatcg gtcattacgt tgcagttcag cctcgatctg
     2941 acactcgacg tcgccgaaca ggaggtgcaa gccgccatca acgcagccac ctcgctgctg
     3001 ccgcaggacc tgccgtcgcc gccgatctac agcaaggtca atcccgccga cacgcccatc
     3061 ctgtctctgg ccatcacctc gagctcgttg ccgctgccca aagtgcagga cctgatcgac
     3121 acccgcatcg cgcagaaaat ttcgcagctt cccggcgtgg ggctggtgag tctgtccggc
     3181 ggccagcgcc ccgcggtgcg cgtccaggcc aatcccaaag cgctggcagc ctatggaatg
     3241 agcctggatg atctgcgtac cgcgatcggc aatgccaacg tcaatcaggc caaaggcagc
     3301 ttcgacgggc cgtcacgcgc ctccacgatc gatgccaacg accagctcaa gtccgccgac
     3361 gagtaccagc gcctgatcat cgcctacaag aacggcgcgc cgatccggct gaccgacgtc
     3421 gcggatgtcg tagacgatgc ggagaacgtg cggctcgccg cctgggccaa cgacaaggcg
     3481 gcgctcatcc tcaacatcca gcgtcagccg ggcgccaatg tgatcgtggt ggtcgatcgc
     3541 atcaagaaac tgcttccgca gttgcaggcc gcgctgcccg gttcggtgga catcgtaatg
     3601 cttaccgacc gaacgacgac aatccgcgcg tcggtcaagg acgtgcagtt cgaactgctg
     3661 ctggcggttg cgctggtggt catggtgatc ttcctgtttc tgcgcaacgt cgccgccacc
     3721 atcattccga gcgtggccgt gccgctgtcg ctcgtgggca ccttcggcgt catttacctg
     3781 gccggattca gcatcaacaa cctcacgctg atggcgctga ccattgccac cggtttcgtc
     3841 gtcgacgacg ccatcgtcat gatcgagaac atcgcgcgct acatcgaaga aggcatgaaa
     3901 ccgctggaag ccgcgctcaa gggcgccgag cagatcggtt tcacgatcat ctcgctgacc
     3961 ttctcgttga tcgcggttct gattccgctg ctgttcatgg gcgacgtggt cggccgcctg
     4021 ttccgcgaat tcgccatcac gctcgcggtc gcgatcctga tttccgcatt cgtgtcgctc
     4081 acgcttacgc cgatgatgtg cgcgcggctg ttgcaccata tccccaaaag cgagcaaagc
     4141 cgcttttacc gctcgagcgg ccgcttcttc gacgcggtga tcgcacgcta cggcaggatg
     4201 ctcgactggg tgctggaccg ccaggccgcc accctgctgg tcgcgctcgg cacgctggcg
     4261 ttgaccgtgc tgctctattt tttcgtgccg aaaggttttt ttccggtgca ggataccggc
     4321 ctgatccagg gcatctcgga agcgccgcaa tcgatctcct tcgcggcgat ggcgcagcgc
     4381 cagcaggacc ttgccaaaat tgtgctgcag gaccccgcgg tggaaagctt gtcgtcgttc
     4441 atcggtgtgg atggcaacaa tgcgacgctg aacagcggcc gcatgctgat caacctcaag
     4501 ccgctgaagg cgcgcggcat cgatgccacc ggagtcatcc gcagattgca accgcaactc
     4561 gcgcaggtcg cgggcatttc gctctacatg cagccggtgc aggacctgac catcgaggat
     4621 cgcgtcagcc gcacgcaata tcagttcagc gtcgaagacg cgaatgcgga caacctgggc
     4681 atctgggtgc cgaaactggt cgagcgcctg cgcgccgcta cgcagctcgc cgatgtcgcc
     4741 agcgacctgc aggaccgggg attgcaggcc ttcgttgaga tcgaccgcag taccgcgagc
     4801 cggctcggca tcacgccggc cgcgatcgac aacgcgcttt acaacgcctt cggccagcgc
//
LOCUS       DASQOP010000110         2177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2058335, whole genome shotgun sequence.
ACCESSION   DASQOP010000110 DASQOP010000000
VERSION     DASQOP010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2058335"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1558)
                     /locus_tag="VE030_04380"
     CDS             complement(<1..1558)
                     /locus_tag="VE030_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZF22689.1"
                     /translation="MDISRFFIDRPIFAAVLSIVIFVAGAIAIPLLPISEYPEVVPPT
                     VVVQATYPGANPKVIADTVSSPLEEQINGVENMMYMKSVAGSDGVLQMTITFAPGTDP
                     NKAQVDVQNRVSQALSRLPSEVVSLGVTTQKQSPAFLTLITLISDGKYDPLYLRNYAN
                     IRVKDQLARIPGVGQIRLFGGGDYAMRVWLDPDKIASRGMTAGDVVRTIQEQNIQVSA
                     GQLSAEPIKGGSDFLIAINAQGRLRTAEEFGDIVLKTGSNGEVVRLSDVARIELGASD
                     YTLRGQLDHYNTSIIGVFQAPGANALATRDAVIAKMDELARQFPPGVSYRSDYDATIF
                     VRESIAAVIHTLFEAIALVVLVVILFLQTWRASIIPLIAVPVSVVGTFAVLYPLGFSI
                     NTLSLFGLVLAIGIVVDDAIVVVENVERNIEEGRTPLEAAHKAMTEVSGPIVAIALVL
                     CAVFVPMAFLTGVTGTFYKQFALTIAISTVISAINSLTLSPALAAKLLHSHDAPKDML
                     SRLIERLLGWF"
     gene            complement(1578..>2177)
                     /locus_tag="VE030_04385"
     CDS             complement(1578..>2177)
                     /locus_tag="VE030_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006752587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZF22690.1"
                     /translation="SRALLTLGNLVQADQTVLTSVVSQDPVYVYFQPDEQSFLGYREL
                     ARKGERANSDNPVRVGLASETGFPHSGTVDFVNNQIDAATGTINVRATVPNPDGVFVP
                     GLYARVQLEGRAEHMAMLIDDKAIMTDQDRKYVYVLGLEDKAKRKDIVLGSVHDGLRV
                     VQSGLDANDKVIVAGLQKIFAPDTKVKPNLVAMGARPQS"
BASE COUNT          419 a          663 c          704 g          391 t
ORIGIN      
        1 agaaccagcc gagcaggcgc tcgatcaggc gcgaaagcat atccttcggc gcgtcgtgcg
       61 aatgcagcaa tttggcggcg agcgccggcg acaaagtcag cgaattgatc gccgagatca
      121 cggtcgagat cgcgatcgtc agggcgaact gcttgtagaa agtgccggtg acaccggtca
      181 ggaacgccat cggcacgaac accgcgcaca acaccagcgc gatcgcgacg atcgggccgg
      241 atacttcggt catcgccttg tgcgcggcct caagcggcgt gcggccttcc tcgatgttac
      301 gctcgacatt ctcgaccacc acgattgcat catcgaccac aatgccgatc gccagcacca
      361 ggccgaacag ggaaagcgtg ttaatggaaa agccgagcgg atagagcacg gcgaaggtgc
      421 cgacgaccga caccggcacg gcgatcaatg gaatgatcga ggcgcgccag gtttgcagga
      481 acaggatcac caccagcacg acgagcgcga tcgcctcgaa cagcgtgtgg atgacggccg
      541 cgatcgattc tcgcacgaag atcgtggcat cataatccga tcggtagctc acgccgggcg
      601 ggaactgcct ggcgagctcg tccatcttcg cgatcaccgc gtcccgcgtc gccagcgcgt
      661 tggcgccggg cgcctggaac acgccgatga tggacgtgtt gtaatggtca agctggccac
      721 gcagggtgta gtcgctggcg ccaagctcga tgcgcgcgac atcggaaagc cgcacgacct
      781 cgccgttcga gccggttttc agcacgatat cgccgaactc ctccgccgtg cgcaggcggc
      841 cctgcgcgtt gatcgcaatc aggaaatcgc tgccgccctt gatcggttcg gcgctgagct
      901 ggccggccga gacctggatg ttctgttcct ggatggtgcg caccacgtcg cccgcggtca
      961 tgccgcgcga agcgatcttg tccggatcga gccagacgcg catcgcatag tcgccgccgc
     1021 cgaacaaccg tatttggccg acgccgggaa tgcgcgcgag ctggtccttg acccggatat
     1081 tggcgtagtt gcgcaggtaa agcggatcat acttgccatc cgagatcagc gtaatcaacg
     1141 tgaggaaggc tggtgactgc ttctgcgtgg tcaccccgag gctgaccact tccgacggca
     1201 ggcgcgacaa cgcctgggaa acgcggttct gcacgtccac ctgcgccttg ttcggatcgg
     1261 tgccgggggc gaaggtgatt gtcatctgca gcacgccgtc ggagcccgct accgacttca
     1321 tgtacatcat gttttcgacg ccgttgatct gctcctccag aggagacgac acggtatccg
     1381 cgatgacctt ggggttggcg cccggatagg tcgcctgcac gacgacggtg ggcggaacga
     1441 cctcgggata ttcgctgatt ggcaatagcg ggatcgcgat cgcgccggca acgaagatca
     1501 cgatcgacag caccgccgca aagatcgggc gatcgatgaa aaatctggag atgtccaagg
     1561 atgcgtctcc ttacgggtta cgactggggc cgggcgccca tcgcaaccag gttgggtttg
     1621 accttggtgt cgggtgcgaa gatcttctgg agaccggcga caatgacctt gtcgttcgca
     1681 tcaaggccgg actgcaccac ccgcaagccg tcatggacgc taccaaggac gatgtccttg
     1741 cgcttggctt tgtcttctag tccgaggaca tagacgtatt tgcgatcctg atcggtcatg
     1801 atggccttgt cgtcgatcag catggccatg tgttcggcgc gcccttcgag ctgcacgcgc
     1861 gcatagaggc cgggcacgaa cacgccgtca gggttcggca ccgtcgcgcg cacgttgatc
     1921 gtgccggtcg ccgcatcgat ctggttgttg acgaagtcca ccgtgcccga atgcggaaag
     1981 ccggtttcgc tggcgaggcc cacccgcacc gggttgtccg agttggcgcg ttcgcccttg
     2041 cgggcgagct cgcgatagcc caggaaggac tgctcgtccg gctggaagta gacatagacc
     2101 gggtcctgcg agacgacgct ggtgagcacg gtctggtcgg cctggacgag gttgccgagc
     2161 gtcagcagcg cgcggct
//
LOCUS       DASQOP010000111         2762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2260082, whole genome shotgun sequence.
ACCESSION   DASQOP010000111 DASQOP010000000
VERSION     DASQOP010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2260082"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="VE030_04390"
     CDS             complement(<1..875)
                     /locus_tag="VE030_04390"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HZF22691.1"
                     /translation="MNSFTLLLVVITVTLVFEFSNGWHDAANSIATVVSTRVLTPLRA
                     VIWAAFFNFVAAFVFGTAVAKTITKGLVHLEMVDEKVILAGLLGAITWNLITLILGLP
                     TSSSHALIAGYGGAAVAHAGFKAIIFGGWLKPVLFIFIAPFIGMFVAVLVTVLTSWIV
                     RRQRPLKVDRWFRRLQLVSAALYSLGHGTNDAQKGMGIITAALVTDGLLKSSEVVPYW
                     VIVCCHLAMAGGTMAGGWRIIKTMGQRITKLNSFGGFAAETASAVTLFATAKFGIPVS
                     TTHTVTGAIVGVGATR"
     gene            complement(879..1505)
                     /locus_tag="VE030_04395"
     CDS             complement(879..1505)
                     /locus_tag="VE030_04395"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HZF22692.1"
                     /translation="MPGLLQRFLPREEGFFVLFAKQAANIHAGAEALLKMLSHYTGVP
                     EQVQIVKAIEHEGDEITHALFTKLNQTFITPFDREDIYELCSRLDDVIDLIDAAASRF
                     VLYRVDTIRIGTIDLVKVLVSATVEVELAIRALEKPQEALKHAIEINRYENDSDRLCR
                     TLIAQLFDEEKDPVQIIKWKEIFEVIETAVDKCEDVANVIEGVILKSA"
     gene            complement(1676..>2762)
                     /gene="groEL"
                     /locus_tag="VE030_04400"
     CDS             complement(1676..>2762)
                     /gene="groEL"
                     /locus_tag="VE030_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HZF22693.1"
                     /translation="FEVVEGMQFDRGYLFPYFITDLERMECVLEDVRILIHEKKISSM
                     KDLLPLLEQTAKMGKPLLIIAEDVEGEALATLVVNKLRGTLQCAAVKAPGFGDRRKAM
                     LEDIATLTGGKAITEDLGIKLENVKLEDLGRAKKITIDKDNSTIIEGAGKSADIEGRV
                     KQIRSQVENTTSDYDKEKLQERLAKLVGGVAVIKVGAATETELKEKKARVEDAMHATR
                     AAVEEGIVPGGGSALLRCIAVVEKLKLHDDEAVGAGIVRRSLEEPSRQIALNAGHEGA
                     VVIGRIRDSKEENFGFNAETGEYGDMIKMGIIDPAKVTRLALQNAASIAGLMLTTEAL
                     IADIKEETKAAAAGGGAPGAGGMGGMY"
BASE COUNT          543 a          720 c          920 g          579 t
ORIGIN      
        1 cgagtagcgc cgacgccgac aatggcgccg gtgacggtgt gcgtggtgct gacagggatg
       61 ccgaattttg cggtggcaaa gagcgtgacg gcgctggcgg tctcggccgc gaagccgccg
      121 aaagaattga gcttggtgat gcgctggccc atggttttga tgatgcgcca gccgccagcc
      181 atggtgccgc cggccatcgc caagtggcaa catacgatga cccaataggg gacgacttcc
      241 gaggacttca gcaggccatc cgttaccaaa gccgcggtga taatccccat gcctttctga
      301 gcgtcgttgg tgccatggcc gaggctatag agagccgcgg agacaagctg caggcggcgg
      361 aaccagcgat cgacctttag cggccgctgc cggcgcacaa tccaactggt aagcaccgtg
      421 actaaaacgg ccacgaacat cccgatgaaa ggggcgatga agatgaaaag aacgggcttg
      481 agccagccgc cgaagataat cgccttgaat ccggcgtggg cgacagcggc acctccgtaa
      541 ccggcgatca gagcgtgaga agagctggtg ggcaggccga ggattagggt gatgagattc
      601 caggtgatgg cgccgagcag gccggcaaga atcacttttt cgtcgaccat ttcgagatga
      661 accagacctt tggtgatggt cttggcgacg gcagtgccga agacgaatgc ggcgacgaaa
      721 ttgaaaaaag cggcccaaat gacggcgcgg agaggcgtga ggacccgcgt ggagacgacc
      781 gtggcgatgg aattggcggc gtcgtgccag ccattggaaa attcaaaaac gagagtgacg
      841 gtgatgacga cgaggaggag agtgaagctg ttcacaggtc aggcgctctt gaggatgacg
      901 ccttcgatga cgttggcgac gtcttcgcac ttgtcgacgg cggtttcgat gacctcgaag
      961 atctctttcc acttgatgat ctggacggga tctttttctt cgtcgaagag ctgggcgatg
     1021 agggtgcggc agaggcggtc gctgtcgttt tcgtaacgat tgatttcgat ggcgtgcttc
     1081 agcgcttctt gcggtttttc gagcgcgcga atggccagtt cgacttcgac ggtggcggaa
     1141 accaggactt tgacgaggtc gatcgttccg atacggatgg tatcgacgcg atagagcaca
     1201 aagcggctgg cggcggcgtc aataaggtcg ataacgtcgt caagccggct gcagagttcg
     1261 tagatatctt cgcggtcgaa gggggtgata aaggtttggt tcagcttggt gaaaagggcg
     1321 tgggtaatct cgtcgccttc atgttcgatg gccttgacga tttgcacctg ttccgggacg
     1381 ccggtgtagt gcgagagcat tttcagcagg gcttcagcgc ccgcatggat attggctgct
     1441 tgtttggcga agaggacgaa gaaaccctct tcgcgcggca agaatcgctg caaaagtcct
     1501 ggcattgttg actctcccct gaagagtcgg tagtgaggca gagggacgat aacagaggcg
     1561 ggtaacggta ggcaagtaag gggattggag ttagagaaga ggggatagac aaacaaaaag
     1621 ggcggcccgt gaggaccgcc ctttaggatt gaggttgtgg gcgagctggt gaaccttagt
     1681 acatgccgcc catgccgcct gcgccggggg ctccgccgcc ggctgccgca gccttggttt
     1741 cttccttgat gtcggcgatg agggcttcgg tggtcagcat gaggcccgcg atggacgctg
     1801 cgttctgcaa cgcgaggcgg gtgaccttgg ccgggtcgat gatgcccatc ttgatcatgt
     1861 cgccgtactc gccggtctcg gcgttgaagc cgaagttttc ttccttggag tcgcggatcc
     1921 tgccgatcac aacggcgcct tcgtggccgg cgttgagagc gatctggcgg gaaggctctt
     1981 cgagggagcg ccgcacgatg cctgcaccaa cggcttcgtc atcgtggagc ttgagcttct
     2041 cgacgaccgc gatgcaacgg agcagcgccg agccgccgcc ggggacaata ccttcctcga
     2101 cagccgcgcg cgtggcgtgc atagcgtctt cgactcgcgc cttcttttcc ttcagttcgg
     2161 tttcggtcgc tgcgccgact ttaatgacgg cgacgccgcc gaccagcttg gcaaggcgct
     2221 cctggagctt ttccttgtcg taatcgctgg tggtgttctc gacctgcgag cggatctgct
     2281 tcacacggcc ttcgatgtcc gcggacttgc cagcgccttc gatgatcgtg gaattatcct
     2341 tgtcgatggt gattttcttg gcgcggccga gatcctcaag cttgacgttc tcgagcttga
     2401 tgccgaggtc ttcggtgatg gccttgccgc cggtgagggt ggcgatgtct tcgagcatgg
     2461 ccttgcggcg gtcgccaaag ccaggggcct tgacggcggc gcactgcaac gtgccgcgga
     2521 gcttgttcac cacgagggtg gccagcgctt cgccttccac gtcctcagcg atgatcaaga
     2581 ggggcttgcc catcttggcg gtctgctcga gcaacgggag cagatccttc atcgagctga
     2641 ttttcttttc gtggatgagg atgcgaacat cttcaaggac gcattccatg cgctcgagat
     2701 cagtgatgaa gtaggggaag aggtagccgc gatcaaactg catgccttcg actacttcga
     2761 ag
//
LOCUS       DASQOP010000112         2621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_647387, whole genome shotgun sequence.
ACCESSION   DASQOP010000112 DASQOP010000000
VERSION     DASQOP010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647387"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1420)
                     /locus_tag="VE030_04405"
     CDS             complement(<1..1420)
                     /locus_tag="VE030_04405"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZF22694.1"
                     /translation="MSSPAESPPGAYQEFAKANQALVEDFLRQLPSSGRLEFTELMRM
                     LAFGSDEQREKLRSLQERLYREHLALWGSLLGGGNDTVQDAAASGDHRFDAPEWSEVP
                     FFRYLRQAYLLNARFLDELGELAPLHGHAKRRLQFVLRQFADAIAPTNFAATNPEVIK
                     LASESGGESLSQGMKLLAADLARGRISMTDERTFEVGRNLAVTPGAVVLENEVMQLIQ
                     YAPSTRQVFERPLLIVPPFINKYYILDLQPENSFARYCVEQGFTTFIVSWRNTPPELG
                     HLTWDDYVREGVLAPLDAVRAITGAGKVNTLGFCVGGTLLATALAVMAHKNDASVASL
                     TLLASMLDFSETGDISVFVDREFVERAEREFRETGSMPGSRLAATFATLRANDLVWRF
                     VINNYLKGREPKPFDLLYWNADGANLPGRLYTYYLRNMYLENSLCVPNRLTMCNTAVD
                     LGNIDMPSYVLATMEDHIVPWKA"
     gene            complement(1508..1648)
                     /locus_tag="VE030_04410"
     CDS             complement(1508..1648)
                     /locus_tag="VE030_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22695.1"
                     /translation="MSATPPVRCSGFLEILPHALEQVSLISLGIGSIALVGFLAKGHG
                     WD"
     gene            complement(1653..2393)
                     /gene="pgeF"
                     /locus_tag="VE030_04415"
     CDS             complement(1653..2393)
                     /gene="pgeF"
                     /locus_tag="VE030_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HZF22696.1"
                     /translation="MLDPSRDWIVPDWPAPANVRALITTRRGGVSTGPFASMNLGQRV
                     DDDLQSVRTNRASLRDLLPAEPKWLMQVHGVDVVNADRLQQLVEADAAVAWNPGSVCA
                     VMVADCLPVLLTDRAGRVVAAAHAGWRGLAAGVVENTVRRMHTAADELLAYLGPAIGP
                     SAFEVGADVRDAFLAWSADAASAFVAHRPGKWLADLFELARQRLRESGVMQIYGGGLC
                     TYSDPRRFFSHRRDKVTGRMAALIWLAD"
     gene            complement(2386..>2621)
                     /locus_tag="VE030_04420"
     CDS             complement(2386..>2621)
                     /locus_tag="VE030_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HZF22697.1"
                     /translation="HQIRVHLHSVGHPLVGDPVYRLNNRSTTLPPEVLDFPRQALHAA
                     RLALVHPQTGQTLTWQAPAPPDIVHLLWILRNA"
BASE COUNT          504 a          802 c          864 g          451 t
ORIGIN      
        1 ccgccttcca cggcacgatg tgatcctcca tggtggccag cacgtagctg ggcatgtcga
       61 tgtttcccag gtcgactgcc gtattgcaca tcgtcaggcg gttcggcacg cacaggctgt
      121 tctcgaggta catgtttcgc aggtaatagg tgtagagacg gcccggcagg ttggctccgt
      181 cagcgttcca gtagagcagg tcgaaaggtt tcggttccct gcctttcaga tagttgttga
      241 tcacgaaacg ccacacaagg tcgttcgcgc gcagcgtcgc aaaggtcgcg gcgagccggc
      301 ttccgggcat cgagccggtt tcgcgaaatt cccgctcggc gcgctcgacg aattcccggt
      361 ccacgaagac gctgatgtcc ccggtttcgg aaaagtccag catcgaggcg agcaaagtga
      421 gcgaggcaac cgaagcatcg ttcttgtgcg ccatgactgc gagcgcggtc gcaagcagcg
      481 tgccgccgac gcagaaaccc agcgtgttga cctttccggc gccggtgatc gcgcgcaccg
      541 catcgagcgg ggccagtact ccttcgcgga cgtagtcatc ccaagtgaga tgtccgagtt
      601 ccggcggggt gttgcgccag gagacgatga aagtcgtgaa gccctgctcg acgcagtagc
      661 gcgcgaagga attttccggc tgcaggtcga ggatgtagta cttgttgatg aacggcggca
      721 cgatgagcag cggccgttcg aaaacctggc gggtggacgg ggcatactgg atcagctgca
      781 tcacttcgtt ctccagtacc accgcgcccg gtgtgactgc gagattacgg ccgacttcga
      841 aggtgcgctc gtcggtcatc gaaatgcggc cgcgcgcgag gtccgcggcg agaagcttca
      901 tcccctgcga caggctttcg ccgccgcttt cgctggcgag cttgatgact tcaggattgg
      961 tcgcggcgaa attggtgggc gcgatcgcgt ctgcgaactg gcgcaacacg aattgcagcc
     1021 gccgcttggc gtggccgtgg agcggcgcca gttcgcccag ttcgtcgagg aaacgcgcgt
     1081 tcaaaaggta agcctgccgc aggtaacgga agaacggcac ctcgctccat tccggagcat
     1141 cgaagcggtg atcgccggaa gcggcggcat cctgcacggt atcgttgccg ccgccgagca
     1201 gactgcccca tagcgcgagg tgctcgcgat agaggcgttc ctggagggag cgcaatttct
     1261 cgcgctgttc atccgaaccg aatgccagca tgcgcatcaa ttcggtgaat tcgaggcggc
     1321 cggatgaggg aagctggcgc agaaaatctt cgaccagggc ctgattggct ttggcgaact
     1381 cctgataggc gccgggaggg gattctgcag gtgaactcat gtcccggtgg acgccggaag
     1441 aatcgaaatc acaacacgtt cgggattctt gaccgcaccc ctggtcaagt caagctgcac
     1501 acgccgctta atcccagcca tgaccctttg cgaggaagcc aaccagcgcg atcgagccga
     1561 tgcccagcga aatgagcgac acctgctcga gcgcgtgcgg caggatttcg aggaaccccg
     1621 agcagcgcac cggtggcgta gctgacaaac cctcagtcgg ccagccagat cagggcggcc
     1681 atgcgaccag tgactttgtc tcgccgatgg gagaagaaac gccgcgggtc ggagtaagta
     1741 cagaggccgc caccgtaaat ctgcatcacc ccgctctccc ggaggcgctg gcgggcgagc
     1801 tcgaacaggt cggccagcca tttgcccggc cggtgggcga cgaatgcgga ggctgcatct
     1861 gcgctccagg ccaagaacgc gtcgcgaacg tcggcgccaa cctcgaaggc ggaagggccg
     1921 atggcaggtc cgaggtaggc gagcagctca tcggcagcgg tgtgcatccg gcgtaccgta
     1981 ttttccacta cgcccgcggc cagcccgcgc cacccggcgt gtgccgccgc gactaccctg
     2041 ccggcgcgat cggtcagcaa caccggcaga cagtccgcca ccatcacagc gcacaccgaa
     2101 cccgggttcc aggcgaccgc ggcgtccgct tccaccagct gctggagcct gtcggcattc
     2161 accacgtcga cgccgtggac ctgcatcagc cacttgggtt cggcgggaag aagatcccgc
     2221 agcgatgcgc ggttggtccg caccgactgt aagtcgtcat cgacgcgttg accaaggttc
     2281 atgctggcga aaggacccgt gctcacgccg cccctgcggg tggtgatgag cgcgcgcacg
     2341 tttgccggag ccggccagtc ggggacgatc cagtcgcgag acgggtcaag catttctcag
     2401 tatccacagc agatgcacga tatccggggg tgccggcgcc tgccatgtca gcgtttggcc
     2461 ggtctgcgga tgaacgagcg cgagcctggc ggcgtgcagc gcctggcggg gaaaatccag
     2521 cacctccgga ggcagggtgg tgcttcgatt gttgaggcga tagaccggat cgccgaccaa
     2581 cggatgtccg accgagtgca aatgcacgcg aatctgatgg g
//
LOCUS       DASQOP010000113        11002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1256507, whole genome shotgun sequence.
ACCESSION   DASQOP010000113 DASQOP010000000
VERSION     DASQOP010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11002
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1256507"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(393..2039)
                     /locus_tag="VE030_04425"
     CDS             complement(393..2039)
                     /locus_tag="VE030_04425"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZF22698.1"
                     /translation="MTSDIVNAPEGNSRHGRTLDALVIGAGFSGLYQLLCLRDRLGLS
                     VQVLEAADGVGGTWYWNRYPGARCDSESHSYCYSFSDELMREWEWSERYPGPPEILRY
                     LNHVADRFDLKRNIRFNTCVTAAHYDEAANRWRATTDAGDVYTAQFLVTAVGCLSAAN
                     VPNIPGLETFSGRWYHTGQWPHEGVDFRGKRVGQIGTGCTGIQVAPVIAETATHLTVF
                     QRTANYSVPARNGPLTPEFKRYVRENTAEIRRVTQSSVNGHPWFINERSALEVSPVER
                     QALYEAAWETGGLRFRGIFRDLVTSNEANATAVAFLKDKIRKIVKDAATAEKLADIDH
                     PYAAKRPPIDTDYFETFNRDNVMLVDVRADPIGRITQNGIRTKSGAEYPLDIIVFATG
                     FDGMTGSLLRMDLRGRDGLPLAEAWKAGPSNYLGLQVAGFPNLFTVTGPGSPSVLCNM
                     PVAIEQHVEWITDCIAHMRKKGLTRIEATEESMEDWVAKVNAAANATVLPQAKHSWYL
                     GANIPGKPRVFMPYAGGMAHYRKICTEVAARNYEGFTLSA"
     gene            2221..2502
                     /locus_tag="VE030_04430"
     CDS             2221..2502
                     /locus_tag="VE030_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HZF22699.1"
                     /translation="MIREAPAMTVRQNLGELLNEVQYRNGKILITKAGKPVAALVDIA
                     LFERIRKLDEEFDQMTADFAKAFAGKGEKTVNALVDEAVKAVRRKPSRK"
     gene            2499..2906
                     /locus_tag="VE030_04435"
     CDS             2499..2906
                     /locus_tag="VE030_04435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HZF22700.1"
                     /translation="MKVVLDTNVLLSGLMFPDGAPGRVVAAWRQVRFELVISVPQLAE
                     IGRTLAYPKIQRILGWDDRRIEQFIRQLYVRAQIVDLAGTSVEVPGDPDDAPILATLV
                     AAKADVLVTGDGDLLALRDRHAIQTPAEFMRRL"
     gene            3054..3683
                     /locus_tag="VE030_04440"
     CDS             3054..3683
                     /locus_tag="VE030_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HZF22701.1"
                     /translation="MGRVKKASAVRTKKPRSLADALFSTTQQRVLGLLFGQPDRSFYA
                     TELIGLAGGGSGAVQRELASLEQSGLVTVRRLGTQKHYQANPDSPLFSELCGIARKTV
                     GLAEPLRAALAPLASRIVMAFVYGSVAKRRDTASSDIDLMVVSDDLSYAEVFEALEAI
                     GARLGRVVNPSVYTRRELAKRVKDGNAFVKRVLAQPKVWVIGTEDDLAA"
     gene            3667..4089
                     /locus_tag="VE030_04445"
     CDS             3667..4089
                     /locus_tag="VE030_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22702.1"
                     /translation="MTSPLERLAGPGKALAAEPPDAKEFAWLKRSGLNRLNDACNTAI
                     SLEGRFDLAYNAAHALCLAALRHHGFRSGNRYVVFEVLPHTLGLGPEVWRVLAKCHEI
                     RNLGEYEGDLDVNERIVTDLISACRAVAAKLGALPPLT"
     gene            complement(4203..5594)
                     /locus_tag="VE030_04450"
     CDS             complement(4203..5594)
                     /locus_tag="VE030_04450"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HZF22703.1"
                     /translation="MNAKLLYAVPVLLSAGFFLFQAQFVSSQTIRARDPGVRGGDPAA
                     GGPIAGLSATEGAFFNTGKDEFEEVDPADEGLGPRMNLDSCGGCHAQPAVGGTSPSVN
                     PQVAFANSLGATNGVPSFITSDGPVREARFQRSPDGTADGGVHALFTLAGRWDAPGCV
                     LTQPDFESELARRNVIFRIPTPLFGAGLIEQIPDSVILANQAADATRKAALGIFGRPN
                     FIVPGNTISGQPNKNGNDGTIARFGWKAQNKSLLLFSGEAYNVEMGITNELFQTERDE
                     TPTCQFATVPNGVTNTNAYTLIEGISAIEKFAFFQRFLAPPTPSPYAPGGALSITRGR
                     SLFNGIGCSACHSPSLQTGNSTVEALRNQPVNLYSDLLLHDMGPRLADGVRQGQAGPR
                     EFRTAPLWGLGQRIFFLHDGRTSDLLEAIYAHQSGTLLNNNASEANEVIKNFNFLLRE
                     SQKQDILNFLRSL"
     gene            complement(5745..6203)
                     /locus_tag="VE030_04455"
     CDS             complement(5745..6203)
                     /locus_tag="VE030_04455"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HZF22704.1"
                     /translation="MGFPESEAQNLALRSDLMIRIEKLVRDSGMTQQQAAKTLGVTQP
                     RLNALLKGKIADFTLHPSPFTHSPFTPSLLFAPSPFTLHAFTAFRPFPLHPSRLHCFS
                     ALHSCAAEASAGGDDEIRWHSMACPSRTASRRFTTTPAGDERAEDFGPSA"
     gene            complement(6334..7506)
                     /gene="dgoD"
                     /locus_tag="VE030_04460"
     CDS             complement(6334..7506)
                     /gene="dgoD"
                     /locus_tag="VE030_04460"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF010624.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HZF22705.1"
                     /translation="MKITAIKTSVCNAEMRNWVFVRVETDQPGLYGWGEATLEWKTRA
                     VVGAVQDLEPLLLGHDPRDIENNVRILTKHSFWRLGVIGNTAISGIEMALWDIWGKEL
                     NVPVWRLLGGKTRDKVKIYTHLGFGDMTAVYESMDRKSVAERALEVIAKGYRALKVVF
                     IPYTHYTASPRELDHVGGMMQALREAVGNDVEIMIDFHGRCASTSVALQYIHALAPYR
                     PLFVEEPIQPGDSLGMKQIMDKSPVPIATGERLVDRREFDDLFRLRAINIAQPDLCHV
                     GGFSEARKIAAMAETAGVGIAPHNPLGPLAGVAALHFDIATPNFVIQEEMSGAVPWYH
                     DVVQSPIKAKDGHWLIPERPGLGVEVSEATVAKHPFKQEIMHAGNAVLADGTIVDW"
     gene            7659..9287
                     /locus_tag="VE030_04465"
     CDS             7659..9287
                     /locus_tag="VE030_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HZF22706.1"
                     /translation="MAQSLGPFDYVIVGAGSAGCLLANRLSADPDVRVLLLEAGGKDD
                     YFWIHVPVGYLYTIGNPRTDWCYKTEPDPGLNGRSLGYARGRVLGGCSSINAMIYMRG
                     QKSDYDHWAALGNKGWSWDEVLPYFKRTEDYQHGADELHGAGGELRVEERRVNWEILD
                     AWREAAEECGIPKIEEFNRGDNFGNAYFQMNQRRGVRWSNTKAFLRPVMHRPNLTVLT
                     RAHVKRVRMQTMGFLKRATGVEFSLGPRGNQFAEAKGEVILAAGAIGSPQILQLSGIG
                     PGALLQDLGINVEHNLPGVGENLHDHLQIRLMYKVKNVRTLNERANSLFGKMAMGLEY
                     LFFRTGPLTMPPSQLGAFAKSDPAQPSANIEWHVQPLSLDKFGSPLHSFPAITPSVCN
                     LRPTSRGHVRIKSSDPNAHPAITLNYLSTIEDKKVAVDSIRFTRRIMQAKALAKYQPE
                     EFKPGPDFKTDIALMSAAGDLGTTIFHPVGTCRMGHDELAVVDDRLRVRGIAGLRVID
                     ASVMPRITSGNTNAPTTMIAEKGAEFVKEDARRR"
     gene            9379..9675
                     /locus_tag="VE030_04470"
     CDS             9379..9675
                     /locus_tag="VE030_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015878014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HZF22707.1"
                     /translation="MPAYLISQIEVHDPVGYEEYRKLVPSSLTKYGGRFIARGGKIDV
                     LEGDWSPRRVVICEFESIERARQWYESEEYRPAMEIRKKTSTARIIVVEGAPPR"
     gene            complement(9933..>11002)
                     /locus_tag="VE030_04475"
     CDS             complement(9933..>11002)
                     /locus_tag="VE030_04475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZF22708.1"
                     /translation="HRLAREALALALDPGTRFRVVDAQTVNINPDSWYAREFGERAGM
                     RIHAAVLLGDGTWITADSTYPPRTTNWFTRLLRNLAIVDGVMLVLLFFAVRLVTKPLS
                     VLARAAEELGRNIERPPLPETGTVELARASRALNTMQDRLKRYVETRLKVLGAMSHDL
                     KTPITRMRLRAEMLEDAELRTRFIRDLDAMQEMVGSTLDYMRGLSEGGEELHPIDLNA
                     LISSLKQDAEEAGHTVTIAGATPGPVMGHAQALKRCLQNLLDNALAYGQRADITVRDE
                     GRAVNVAIADSGPGIPERDIERVFDPFYRVEGSRNRNSGGSGLGLSIARNIAQAHGGA
                     VRLRNLPQGGLEATLVLPRGA"
BASE COUNT         2079 a         3391 c         3430 g         2102 t
ORIGIN      
        1 acctccgggg cagacccctt tgaaggaatt gttattcagt aatacaactc cgcgcttgct
       61 acgcaagcct cgcctgctgg actgccggcg cacatcatcc agcgcggaaa cgataaggtc
      121 gtgatggcgt gaatgagtga agggtgaagg gtaacgtcgc aagagcccag tcccactgac
      181 ttcatcagtt gcccttgatc ggggctatct gtcgttccga ctaagaatct ttcgatccgc
      241 tatgtgttga tggccaatca ctttcatctg ctgctcagtg aacgggatct tgcctgtgta
      301 gccgtccacg attcttgcaa gcggttgtca aatccgttat ttcccgacct tgaggaagct
      361 attcgaatct tcgcactccc gccccgtgaa gctcaagcgc tcagggtgaa gccctcgtag
      421 ttcctcgcgg caacctcggt gcagatcttc cggtagtgcg ccatcccgcc ggcgtacggc
      481 atgaaaacac gcggcttgcc gggtatgttc gccccgaggt accaggagtg tttggcctgc
      541 ggcagcacgg ttgcgttggc agctgcattg accttcgcta cccagtcttc catggattcc
      601 tccgtagcct cgatgcgcgt caaccctttc tttcgcatgt gggcgatgca atccgtgatc
      661 cactccacat gctgctcgat cgcgaccggc atgttgcaaa gcaccgacgg actgccgggc
      721 ccggtcacgg tgaagaggtt cggaaatcca gcaacctgca ggcccagata attgcttggc
      781 ccggctttcc aggcttccgc caatggcagg ccgtcgcgcc cgcggaggtc catgcgcaat
      841 agcgagccgg tcatgccgtc gaatcccgtg gcgaagacga tgatgtccag cggatactcg
      901 gccccgcttt tcgttcgaat gccgttttgc gtgatgcgcc cgatggggtc agcgcgcaca
      961 tctaccagca tcacgttgtc gcgattgaag gtctcgaagt agtccgtgtc gatcggcggc
     1021 cgcttcgcgg catagggatg gtcgatgtcg gcaagcttct ccgccgtggc cgcatctttc
     1081 acgatcttcc ggatcttgtc cttgaggaag gcgaccgcag tggcgttcgc ctcgttgctg
     1141 gtcaccaggt cgcggaagat cccgcgaaat cgaagcccgc cggtctccca ggctgcttcg
     1201 tacaaggcct gccgttctac gggtgagacc tccagggcgg agcgctcgtt gatgaaccac
     1261 ggatggccat tgacggagga ttgcgtcacc cgccggatct ctgccgtgtt ctcccttacg
     1321 tagcgcttga actccggggt cagtggacca ttgcgggccg gcacgctgta atttgcggtg
     1381 cgctggaaga cagtcagatg agtggcggtc tcggcgatca ccggtgcaac ctggatgccg
     1441 gtgcaaccgg tgccgatctg cccgacgcgt ttgccgcgga agtccacgcc ttcatgcggc
     1501 cattgccctg tgtgatacca gcggccggag aaagtctcga ggcccgggat gttcggaaca
     1561 ttggccgccg acaggcagcc gacagcggtg accaggaact gcgcggtgta gacatcgccg
     1621 gcgtcggtgg tcgcgcgcca gcggttcgct gcttcatcgt agtgcgcggc cgtcacgcag
     1681 gtattgaagc ggatgttccg cttcaggtcg aagcggtcgg cgacatggtt gagatagcgc
     1741 aggatctccg gcgggccggg atagcgttcc gaccattccc attcccgcat caactcatcg
     1801 gagaacgagt aacagtagga gtggctttcc gaatcgcaac gcgcaccggg gtaacggttc
     1861 cagtaccagg tccctccgac cccgtcggcc gcctccagca cctggactga aagaccaagc
     1921 cgatcgcgca ggcaaagcaa ttgatagagg ccggaaaacc cggcgccgat gaccagcgca
     1981 tcgagggtgc gaccgtggcg cgaattgccc tccggagcgt tcacgatgtc gcttgtcatt
     2041 gccattcgaa tccttgatcg atctcgattg caatcctgaa tgcattatcc cgcaatggcc
     2101 ggcagcatgg cggcgctttc caagatcggc ttggtgcgat aaagataagg ggcgaacttc
     2161 gggcattcaa tcatgtcaat tgatataatt gcatcaacca tatcaattga aggagagagc
     2221 atgatccgcg aagcccccgc catgacggtc cggcagaatc tgggcgaact gctcaatgag
     2281 gtccagtatc gaaacggcaa aatcctgatc accaaggccg gcaagccggt ggcggcgctg
     2341 gtggacatcg cccttttcga gcggatccgg aagctggacg aggaattcga ccagatgacg
     2401 gcggatttcg cgaaggcgtt cgcaggaaaa ggcgagaaga cggtcaatgc cctggtggat
     2461 gaggcggtga aggccgtacg ccgcaagccc tccaggaagt gaaggtcgtc ctcgacacca
     2521 atgtgctgct gtccggcctg atgtttccgg atggcgcgcc ggggcgggtc gtggccgcct
     2581 ggcgtcaggt gcgattcgag ctcgttattt cggtacccca gctggcggaa atcggcagga
     2641 cgctggccta tccgaagatc caacgcatac tcggctggga cgacagacgc atcgagcagt
     2701 tcatccggca gctctatgtc agagcccaga tcgtggatct cgccggaacc tcagtggaag
     2761 tgcccggcga tccggacgat gcccctatcc tggccacgct cgttgccgca aaggcggatg
     2821 ttctggttac gggagacggc gatttgctcg cgttgcgtga tcgacatgct atacagacgc
     2881 ccgcggaatt tatgcgcagg ctgtgaacgt gaagggtgaa ggatcaagga tgaaggcgga
     2941 aggatgaagg atgaagaaaa acacggacga gggatgaagg gcgggaagaa catcagaact
     3001 gactaaaggc gaagaacttg aacataccca gaatgggtag taatatgccc agaatgggta
     3061 gggtcaaaaa agcaagtgcc gtacgcacga aaaaaccgcg aagcctggcg gacgcactgt
     3121 tctccacgac gcagcagcgc gtgctcgggc tgttgttcgg tcagccggat cgcagcttct
     3181 acgcgaccga actgatcggt ctggcggggg gtggctccgg tgcggtccag cgggaactgg
     3241 cgagtcttga acagagcggt ctggtgaccg ttcgccggct gggcacacag aaacactacc
     3301 aagcgaaccc ggactcgcca ctcttcagcg agttgtgcgg catcgcgcgc aagacggtcg
     3361 gtctggcgga gccgttgcgt gcggcactgg cgccgttggc ttcccgcatc gtcatggcct
     3421 tcgtgtacgg ctccgtggca aaacgccggg acacggcatc cagcgacatc gatctgatgg
     3481 tcgtgagcga cgacctgagc tacgccgaag tttttgaagc gctggaggcg attggcgcca
     3541 ggctcggtcg tgtggtcaac ccctccgtgt atacccgtag ggaattggcc aaacgcgtca
     3601 aggatggcaa cgccttcgtg aagcgcgtgc tcgcgcagcc gaaggtctgg gtgatcggga
     3661 cggaagatga cctcgccgct tgagcgcctg gccgggccgg gcaaagcact cgcggcggag
     3721 ccgccggatg cgaaagaatt cgcctggctc aagcgctccg ggttgaaccg cctcaatgac
     3781 gcctgcaaca cggcgatctc actcgaaggg cgcttcgacc tcgcctacaa tgccgcgcac
     3841 gcgctgtgcc tggctgcatt gcgccatcac ggattccgat ccggcaatcg ctacgtcgtg
     3901 ttcgaagttt tgccgcatac gctcggcttg gggccggagg tctggcgggt gctggcgaag
     3961 tgccacgaga tccgcaacct gggcgaatac gagggtgacc tcgacgtcaa cgaacggatt
     4021 gtcacggact tgatctcagc ctgtcgtgcc gtggctgcca aacttggcgc cttgccaccg
     4081 ttgacctgag gcaggccgct tttcgtccgc cccgcgaagc ccacggggtc aaagcccacg
     4141 ggctcaggtc ttccgtgtcc cgatccttcg tctttcctcg cccctttgct tttccgcgac
     4201 ggctacagcg agcgcaggaa attgaggatg tcctgcttct ggctctcccg caggaggaag
     4261 ttgaagttct tgatgacctc gttcgcttca gacgcgttat tgttgagcaa agtcccgctc
     4321 tgatgagcat agatcgcttc cagcaagtcg gacgtccgtc cgtcgtgcag gaaaaaaatc
     4381 cgctgtccca agccccacag cggggcggtc cgaaactccc tgggtccggc ctgcccttgt
     4441 ctgaccccat cggccaggcg cgggcccatg tcatgaagca acagatcgga gtaaagattg
     4501 acgggctgat tgcgcagcgc ctcgaccgtg gaattgccgg tctggaggga tggcgagtga
     4561 cacgccgaac acccgatgcc gttgaacagg ctgcggcctc tggtaatgga cagagccccg
     4621 cccggtgcat agggagaagg tgtcggcggc gccaggaaac gctggaagaa ggcgaacttc
     4681 tcgatcgcac tgattccttc gatgagcgta taggcattgg tgttcgtgac gccattaggc
     4741 accgtcgcga actggcatgt cggggtttcg tcccgctcgg tctggaagag ctcgttggtg
     4801 attcccattt ctacgttata ggcttcgccg gagaacagga ggagcgactt gttctgagcc
     4861 ttccacccga accgggcgat cgtgccgtca ttgccgttct tgttcggctg accggagatg
     4921 gtattgcccg ggacgatgaa attcggccgg ccgaagatcc cgagcgccgc cttgcgcgtt
     4981 gcgtcggcgg cctggttcgc gaggatcacg ctatccggga tctgttcgat caggcccgct
     5041 ccgaacagcg gggtgggaat ccggaagatc acgttgcggc gtgcgagctc gctctcgaag
     5101 tcaggttgcg tgagtacaca gcccggcgcg tcccagcggc cggcgagcgt aaacagagcg
     5161 tggacaccac catccgccgt tccgtcgggg cttctctgga accttgcctc acgcactggc
     5221 ccgtctgacg tgatgaatga tggaaccccg ttggttgcgc ccaagctgtt ggcgaacgca
     5281 acctggggat tgactgacgg gctcgttccg ccgaccgccg gctgcgcatg gcaaccgccg
     5341 cagctgtcca ggttcatcct cggcccaagg ccttcgtcgg ccggatccac ttcctcgaat
     5401 tcgtccttgc ctgtgttaaa aaacgcgcct tccgtcgcgc tcaatcctgc gataggtccg
     5461 cccgcagcgg ggtcgccgcc gcgcacgccg ggatctcgtg ctctaatcgt ctgggaggat
     5521 acaaactgcg cctggaacaa gaagaatccg gctgacagca agacgggaac cgcgtagagc
     5581 aacttagcgt tcatggcttt gtctccgtgt caggaatgcg ccgcgaacca cccccgtgcg
     5641 tgcggggtac ccaatcaatc cgcagccggt ttcttgttct tcgcctcggc cgcggtaatg
     5701 caaatcacgc acggctcatg ccagacggtg tcttgtgatc acaatcaggc agatgggcca
     5761 aagtcttcgg ctcgctcgtc accagcgggc gtcgttgtga aacgacgcga tgcagttctg
     5821 gaaggacacg ccattgaatg ccagcgtatt tcatcgtcgc cgccggcaga cgcttctgct
     5881 gcgcaagagt gaagggccga aaagcagtga aggcgtgaag ggtgaagggg gaaggggcga
     5941 aatgcagtga aggcgtgaag ggtgaagggt gaaggggcga aaagcagtga aggcgtgaag
     6001 ggtgaatgag tgaagggtga agggtgaagg gtgaaatctg cgatttttcc cttgagcaac
     6061 gcgttaagcc tgggctgggt aacgccaagc gttttggcgg cctgttgctg cgtcatgccg
     6121 ctatcccgca ccagcttttc aatgcggatc atcagatctg aacgcagggc gagattctgt
     6181 gcttcgctct caggaaagcc cagatcggcg aagacattgc ggctgctttt ttgtaagtac
     6241 ggttcatgtg ctcatttgtg cttcctttcg tttgccagtt gccgaaatcg ctttgcggca
     6301 agctcaatgt cccgccggag tgtcttctcg cgttcaccaa tccacgatcg tgccgtccgc
     6361 cagcaccgcg ttgccggcgt gcatgatctc ctgcttgaac ggatgtttcg ccaccgtcgc
     6421 ctcgctgact tcgacgccga ggccggggcg ttcggggatc agccagtgcc cgtccttggc
     6481 cttgatcggg ctttgcacca cgtcgtgata ccacggcacc gcgcctgaca tttcttcctg
     6541 gatcacgaag ttcggcgtgg cgatgtcgaa gtgcagcgcc gccaccccgg caagcggccc
     6601 gagcggattg tgcggggcga tgccgacacc cgcggtctcc gccattgccg cgatcttgcg
     6661 cgcttcgctg aagccgccga cgtggcacag gtcgggctgg gcgatgttga tcgcgcgcag
     6721 gcggaacagg tcgtcgaatt cgcggcgatc caccaggcgt tcgccggtgg cgatcggcac
     6781 cggcgatttg tccatgatct gcttcatgcc cagggaatcg ccgggctgga tcggttcctc
     6841 gacaaacagc ggccggtacg gcgccaacgc gtggatgtac tgcagcgcca cgctggtcga
     6901 ggcgcagcgg ccgtgaaaat cgatcatgat ctcgacgtcg ttgccgaccg cctcgcgcaa
     6961 cgcctgcatc atgccgccga cgtggtccag ttccctgggc gatgcggtgt agtgcgtgta
     7021 agggatgaac acgactttga gcgcgcgata gcccttcgcg atcacttcca gcgcgcgctc
     7081 cgccaccgac ttgcgatcca tgctctcgta caccgcagtc atgtcgccga agccgaggtg
     7141 ggtgtagatc ttgaccttgt cgcgggtctt gccgcccagc aggcgccaca ccggtacatt
     7201 gagttccttg ccccagatgt cccacagcgc catctcgatg ccgctgatgg cggtgttgcc
     7261 gatcaccccc agccgccaga acgagtgctt ggtaagaatc cgcacattgt tctcgatgtc
     7321 gcgcgggtcg tgtccgagca gcagcggttc caggtcctgg accgcgccga ccaccgcgcg
     7381 ggtcttccat tcgagcgtgg cttcgcccca gccgtacagc cccggctggt cggtttccac
     7441 ccgcacgaac acccagttgc gcatctcggc gttgcagacc gaggtcttga tcgcggtgat
     7501 tttcattttt ctcctcccgg gggacactct gaaggcgaaa gattaaagcc aagccccgat
     7561 cttatccagc gcaccgattc gggcataatc tctgccgccg caaggggcgc cgcgtgcgga
     7621 ctgtgcggat tcatccatat atatagcgaa aggaagcaat ggcccaatct ctgggaccat
     7681 tcgattacgt catcgtcggc gccggctcgg ccggctgttt gctcgccaat cgcctgagtg
     7741 ccgatccgga cgtgcgcgtg ctcctgctcg aggccggcgg caaggacgac tacttctgga
     7801 tccacgttcc ggtcggttat ctctacacca tcggcaaccc gcgcaccgac tggtgctaca
     7861 agaccgaacc cgacccggga ctgaacggcc gttcgctcgg ctatgcccgc gggcgcgtgc
     7921 tcggcggctg ctcctcgatc aacgcgatga tctacatgcg cggacagaag agcgattacg
     7981 accactgggc cgcgctcggt aacaagggct ggtcgtggga cgaagtgctg ccctacttca
     8041 agcgcaccga ggattaccag cacggcgccg acgagctgca tggcgccggc ggcgaactgc
     8101 gcgtcgagga gcggcgagtg aactgggaga tcctcgatgc ctggcgcgag gccgcggagg
     8161 aatgcggtat acccaagatc gaggaattca accgcggcga caatttcggc aacgcctatt
     8221 tccagatgaa ccagcggcgc ggcgtgcgct ggagcaatac caaggccttt ctgcggccgg
     8281 tgatgcaccg gccgaacctg actgtgctca cccgtgcgca tgtgaagcgc gtgcgcatgc
     8341 aaaccatggg atttctcaag cgcgccaccg gcgtagagtt cagcctcggc ccgcgcggca
     8401 accagtttgc cgaagcgaag ggagaagtga tcctcgccgc cggcgcgatc ggctcgccgc
     8461 agatccttca gctctccggc atcggccccg gcgcgctgct gcaggacctc ggcatcaacg
     8521 tcgagcacaa cctgccaggg gtgggcgaaa acctgcacga ccacctgcag atccggctga
     8581 tgtacaaggt gaagaacgtc cgcaccctca acgagcgcgc caacagcctg ttcgggaaaa
     8641 tggcgatggg gctcgagtac ctgttcttcc gcaccgggcc gctgaccatg ccgccctcgc
     8701 aactgggcgc gttcgcgaaa agcgatccgg cgcagccgtc ggcgaacatc gaatggcacg
     8761 tgcagccgct gtcgctggac aagttcggct cgccgctgca cagctttccc gcgatcacgc
     8821 cttcggtatg caacctgcgc cccaccagcc gcggccacgt gcgcatcaag agctccgacc
     8881 cgaacgcaca tccggccatc acgctcaact atctttccac catcgaggac aagaaagtcg
     8941 cagtggactc gatccgcttc acccgccgca tcatgcaggc caaggcgctg gcgaaatacc
     9001 agccggagga attcaaaccc gggccggatt tcaagaccga catcgcgctg atgagtgcgg
     9061 ctggcgatct cggcacgact atcttccacc cggtcggcac ctgcaggatg ggccacgacg
     9121 aactcgcagt cgtggacgac cgcctgcgcg tgcgcggcat cgccgggctg cgcgtgatcg
     9181 atgcatcggt gatgccgcgc atcacctccg gcaacaccaa cgcgccgacc accatgattg
     9241 ccgagaaggg cgccgagttc gtgaaggaag atgcccggag gcgctgaaac ggcattcagc
     9301 gccgccagct tccggatcta ccgctccccg tcgccattac ggtttcgcag agctaacttt
     9361 ttctttggag gacaaatcat gccggcttac ctgatttcgc agatcgaggt tcacgacccc
     9421 gtgggttacg aggagtaccg caagctcgtc ccgtcgtcgc tcacgaagta cggcggtaga
     9481 ttcattgcgc gcggtggcaa aatcgacgtg ctcgaaggcg actggtcgcc caggcgcgtg
     9541 gtgatctgcg agttcgaaag catcgaacgc gcgcgccaat ggtacgaatc agaggaatac
     9601 cggccggcaa tggagatccg caagaagacc tcgaccgcac ggatcatcgt cgtcgagggc
     9661 gccccacccc gctaacccgc cctcgcccct ctccacaatt cgaacgactc accaataacg
     9721 cactcaccgc cagggacgcc aaggacgcca gggaaaagca tatggggtga aagcgcggct
     9781 gagttagggt gacgcaacac agatactggc acggggattg gacactcacc tgatccttat
     9841 atttgccttt ccctggcgtc cttggcgtcc ctttgcgtcc tttgcgtcaa cgctgtggtc
     9901 gccggccaat ggccggccat gattgaagcg cgtcaggcgc cccgcggcag cacaagtgtg
     9961 gcctccagcc cgccctgcgg caggttacgc agccgcaccg cgccgccgtg cgcctgagcg
    10021 atgttgcggg caatcgacaa tcccagcccg ctgccgccgc tgttgcggtt gcgcgagcct
    10081 tcgacgcgat agaacggatc gaagacgcgc tcgatgtcgc gttccgggat gcccggaccg
    10141 ctgtcggcga tggcgacatt caccgcccga ccttcgtcgc gcaccgtgat gtcggcgcgc
    10201 tggccgtaag ccagcgcatt gtccagcagg ttctgcaggc aacgcttgag cgcctgcgcg
    10261 tgccccatca ccgggcccgg cgttgcgccg gcaatggtga cggtatggcc ggcctcctcg
    10321 gcgtcctgtt tcaggctcga gatcagggcg ttgagatcga tcggatgcaa ctcttccccg
    10381 ccttcgctca ggccccgcat gtagtccagg gtggacccaa ccatctcctg catcgcgtcg
    10441 aggtcgcgga taaagcgcgt cctgagctcg gcgtcttcca gcatttcggc gcgcagccgc
    10501 atgcgcgtga tcggcgtctt gaggtcatgc gacattgcgc ccaatacctt gagccgcgtc
    10561 tcgacgtagc gcttcaggcg gtcctgcatg gtgttgagcg cgcgcgacgc gcgcgcaagt
    10621 tccaccgttc ccgtttccgg aagcggcggc cgctcgatgt tgcggccgag ttcctccgcc
    10681 gcccgcgcca gcaccgacaa gggtttggtg accagtcgca ccgcaaaaaa gagcagtacc
    10741 agcatgacgc catccacgat cgcaagattg cgcagcaacc gggtgaacca gttcgtcgtg
    10801 cgcggggggt aggtgctgtc cgcggttatc caggtgccgt cgccaagcaa tacggcagca
    10861 tggatgcgca tcccggcacg ctcgccgaat tcacgcgcgt accaggaatc cgggttgatg
    10921 ttcaccgtct gcgcgtcgac cacccggaat cgcgtgccgg gatcgagcgc cagtgccagc
    10981 gcttcgcgcg ccaggcggtg ga
//
LOCUS       DASQOP010000114         4682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2865130, whole genome shotgun sequence.
ACCESSION   DASQOP010000114 DASQOP010000000
VERSION     DASQOP010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4682
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2865130"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VE030_04480"
     CDS             complement(<1..371)
                     /locus_tag="VE030_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZF22709.1"
                     /translation="MFGIHAALKPTYDAVIIGAGGHGLAAAYYLARDHGIGNVAVLER
                     AYIGGGNTGRNTTIIRSNYLTPEGVAFYDESLRLWQDLAADFDLNLFYSTRGHFTLAH
                     TDSAVRTMRWRAEVNKHLGVD"
     gene            605..1927
                     /gene="gshA"
                     /locus_tag="VE030_04485"
     CDS             605..1927
                     /gene="gshA"
                     /locus_tag="VE030_04485"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HZF22710.1"
                     /translation="MTVPRLATAQIGPVLSLEKQILDGMPSIEHWFRGQWQEHGAPFY
                     ASVDLRNSGFKLAPVDTNLFPGGFNNLNKEFLPLCVQAAMTAIEKVCPDARGFLVIPE
                     NHTRNIHYLQNVAALQNILQLAGMKVRIGSLLPEITAPIALDLPDGKKLVLEPLVRRG
                     NRLGLADFDPCTILLNNDLSAGVPDILKNLEQPVIPPLFAGWSTRLKSNHFAAYKEVA
                     EEFAGLLGIDSWLINPYFATCGEINFQERVGEECLSGWVDHILTQTRAKYREYGIQYE
                     PFVIVKADAGTYGMGIMTVKDASEVQGLNRKQRNKMAVVKEGLQVSNVMVQEGVYTFE
                     TVNDGVAEPVIYMIDHFVVGGFYRVHTQRDVDENLNAPGMRFEPLAFEDSCSLPDLDG
                     NPDCAPNRFYAYGVVARLALLAAAIELEHMEAALEQQPFPQAMVAANT"
     gene            1986..2936
                     /gene="gshB"
                     /locus_tag="VE030_04490"
     CDS             1986..2936
                     /gene="gshB"
                     /locus_tag="VE030_04490"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HZF22711.1"
                     /translation="MNIAFVLDPLDTIKTYKDSSYAMMREAAARGHALAALQQEDLIW
                     RDGRVLGYARDLSLTSNDKHWYHVGDRKPVPLETFDVVLMRKDPPFDMEYLYSTYLLE
                     LAEKQGARVVNSPRAVRDHNEKLAIARFAQFTIPTLVTRVEQLIRDFLAEHGDIILKP
                     LDGMGGASVFRLRGQDPNIGVVIETLTHYGRRTVMAQRFIAEIVKGDKRVLLIDGKPV
                     PYSLARIPKQGETRGNLAAGATGVAQPLTSRDREIAEAVGPELKAQGLMLVGLDVIGD
                     YLTEINVTSPTCMREIADQTGFNVAALMIDALEKAVKGKA"
     gene            3128..3544
                     /locus_tag="VE030_04495"
     CDS             3128..3544
                     /locus_tag="VE030_04495"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HZF22712.1"
                     /translation="MVGILIVAHGTLGESLIHCASHVMGSRPPYLMQIGVSMHDDPQL
                     VLPQAIKLVRSLDQGGGVLVLSDAYGATPCNIASKLLIPGKIEGVAGVNLPMLVRALT
                     YRKEPLRTVVAKAISGGIEGVMQIPAAQVKNAATGN"
     gene            3525..3794
                     /locus_tag="VE030_04500"
     CDS             3525..3794
                     /locus_tag="VE030_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010101417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HZF22713.1"
                     /translation="MQQREIEITNKLGLHARASAKLTQLAGKFAADVWVTRDDRRVNA
                     KSIMGVMMLAAAKGATVVVETNGPDEAEAMMALVGLINDRFGEGE"
     gene            3901..>4682
                     /locus_tag="VE030_04505"
     CDS             3901..>4682
                     /locus_tag="VE030_04505"
                     /inference="COORDINATES: protein motif:HMM:NF017346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HZF22714.1"
                     /translation="MSFTIHGIGVSGGIAIGHAQLVSHAQLEVAHYNISESRVADEQA
                     RFDAALVTARAELASLGQHVPAGAPAEFEAFLNLHLMILDDPTLSEVPKQLIETQQCN
                     AEWALKLQMEALMDQFERIEDGYIRERKTDVIQVVERVLKVLMGRPGEVLAHSAPEEN
                     SILVAHDLSPADVILFKSHKFASFVTDLGGATSHTAIIARSLNIPSVVALHHARTLIR
                     ENEVLIVDGTNGVVIINPDRTVLGEYKLRQEQWELERLKLRRL"
BASE COUNT          958 a         1430 c         1440 g          854 t
ORIGIN      
        1 ctgtcaacgc cgaggtgctt attgacctcg gcacgccagc gcatggtgcg gaccgccgag
       61 tcggtgtggg cgagggtgaa atgtccgcgc gttgagtaaa agagattcag gtcgaagtcg
      121 gcagccaggt cctgccacag gcgcaatgac tcgtcgtaaa acgccacgcc ctcgggggtc
      181 aggtaattcg agcggatgat ggtggtattg cggccggtgt tgccgccgcc gatataagcc
      241 ctctccagga cggcgacatt gccgatcccg tggtcccttg cgagatagta agcggccgcc
      301 aacccgtgcc cgcccgcgcc gatgattacc gcgtcgtagg tcggtttgag cgccgcgtgg
      361 attccgaaca ttctcggttc ggagtagcga tgggccaggg caaagcgcaa caaccgtagg
      421 ggcatggcgg caagtcagga tttgcgagcg cgagaaacgg cttccatact ggattttttt
      481 tgatttcgag tcaacaccaa taggaatctt tgtttatcaa agagaaactt ctgaattggt
      541 attggaaagc gggttgcgaa gacgtgtctg ctacaatccg gccgaaccca acctgttgac
      601 gcacatgacc gttccccgcc ttgctaccgc ccagatcggc cctgttcttt cgctcgagaa
      661 gcagattctc gacggcatgc cgagcatcga gcattggttc cgcggccaat ggcaggagca
      721 tggggcaccg ttttacgcat cggtggatct gcgcaacagc ggcttcaagc tggcgccggt
      781 ggataccaac ctgttccccg ggggattcaa caacctgaac aaggaattcc tgccgctgtg
      841 cgtacaggcc gcgatgaccg cgatcgaaaa ggtgtgcccg gacgcacgcg gttttctggt
      901 aatccccgag aaccataccc gcaacatcca ttatctgcag aacgtggcgg cattgcagaa
      961 catcctgcag ctcgccggca tgaaggtgcg catcggctcg ctgttgccgg agatcaccgc
     1021 gccgatcgcg ctggacctgc ccgacggcaa gaagctcgtg ctggagccgc tggttcgtcg
     1081 cggcaaccgc ctcgggctgg ccgatttcga cccctgcacc attctgctca acaacgattt
     1141 gtcggccggc gtcccggaca tcctcaagaa cctcgagcag ccggtgattc cgccgctctt
     1201 cgcaggctgg tcgacacggt tgaaatcgaa ccatttcgcc gcgtacaagg aggtcgccga
     1261 agagttcgcc gggctgttgg gtatcgactc ctggctgatc aatccctact ttgccacctg
     1321 cggcgagatc aacttccagg agcgcgtcgg cgaggaatgc ctctcaggct gggtcgacca
     1381 catcctgacc cagacgcgcg cgaaataccg cgagtacggc atccagtacg agcctttcgt
     1441 gatcgtcaag gccgacgcag gcacttatgg gatggggatc atgaccgtca aggacgcctc
     1501 cgaagtgcag gggttgaatc gcaagcagcg caacaagatg gcggtggtga aggaaggcct
     1561 gcaggtttcc aacgtaatgg tgcaggaagg cgtctacacc ttcgagaccg tcaacgacgg
     1621 ggtcgccgaa ccggtcattt acatgatcga tcactttgtg gtcggcggct tctatcgcgt
     1681 gcacacccag cgcgacgtcg acgagaacct caacgcaccg ggcatgcgct tcgagccgct
     1741 cgctttcgag gacagctgct cgcttccaga cctggacggc aatcccgact gtgcacccaa
     1801 ccgcttttac gcgtacggcg tggtagcgcg gctggccttg ctcgccgccg cgatcgagct
     1861 ggagcacatg gaagcggctc tcgaacagca gccgtttccg caggccatgg tggctgccaa
     1921 cacctgaacg cggttccggc acgcttgcgg gtgcggcgca gttggcgcgc tcccgcgata
     1981 acaacatgaa tatcgccttc gttctcgatc cgctcgacac catcaagact tacaaggact
     2041 cttcctacgc gatgatgcgc gaggcggcgg cccgcggcca tgcgctcgca gcgctgcagc
     2101 aggaggacct gatctggcgc gatggccgcg tgcttggcta cgcgcgcgac ctttcactca
     2161 cctccaacga caagcactgg taccacgtcg gcgacaggaa gccggtgccg cttgagactt
     2221 tcgatgtggt attgatgcgc aaggacccgc cgttcgacat ggaatacctc tacagcactt
     2281 atctgctgga gctggcggaa aaacagggcg cgcgagtcgt caacagcccg cgtgccgtgc
     2341 gcgaccataa cgagaaactg gcgatcgcca ggtttgcgca gttcacgatt ccgaccctgg
     2401 tcacgcgcgt ggagcagttg atccgcgact ttctcgccga gcacggcgat atcatcctca
     2461 aaccgcttga cggcatgggc ggcgcttcgg tgttccgcct gcgcgggcag gatcccaaca
     2521 tcggggtggt gatcgaaacc cttacccatt acggccggcg aaccgtgatg gcgcagcgct
     2581 tcatcgccga gatcgtcaag ggagacaaac gcgtcctgct gatagatggc aagcccgtgc
     2641 cctactcgct tgcgcgcatt cccaaacagg gggaaacccg cggcaatctc gctgccggtg
     2701 ccaccggcgt ggcgcagccg ttgacttccc gcgaccggga gatcgcagag gccgtcggcc
     2761 ccgaactcaa ggcacaggga ctgatgctgg tcgggctgga cgtgatcggc gattacctga
     2821 ccgagatcaa cgtcaccagc cccacctgca tgcgtgaaat tgccgaccag accggattca
     2881 atgtcgcggc gctgatgatc gatgcgctgg aaaaagcggt gaagggcaag gcgtgagagg
     2941 tgagcggttg aggggtgatc aagttattaa gtgattgagt gactggcttc aatcacctaa
     3001 ttactgaatc acctaatcac cgattcttgg cccctcgccc ctcagctgac atctctcgcc
     3061 tttcttcgct caggcactcc gggggtagaa tctctacggt attctccgct gcggttcaaa
     3121 gggacttatg gttggaatac tgatcgttgc ccacggcacg ctgggtgaga gcctgattca
     3181 ctgcgccagc cacgtgatgg gcagccggcc gccgtaccta atgcagatcg gggtgagcat
     3241 gcacgacgat cctcagctag tgctgccaca ggcaataaag ctggtgcgca gcctcgacca
     3301 gggcggcggc gtactggtgc tttccgacgc ttacggtgcc acgccgtgca acatcgccag
     3361 caagttgctg atcccgggca agatcgaagg tgtcgcgggg gtaaatctgc cgatgctggt
     3421 gcgcgcactg acttatcgca aggaacccct gaggaccgtg gtcgcgaaag cgatctccgg
     3481 gggcatcgaa ggcgtcatgc aaattcccgc cgcgcaggtg aaaaatgcag caacgggaaa
     3541 ttgagatcac caacaagctg ggactgcacg cgcgcgcgtc ggccaagctc acgcagctcg
     3601 ctggcaaatt cgccgccgat gtatgggtca ctcgcgacga ccgccgggtc aatgccaaga
     3661 gcatcatggg cgtgatgatg ctcgccgccg ccaaaggcgc gaccgtcgtg gtcgagacca
     3721 acggtccgga tgaagccgag gcgatgatgg cgttggtcgg gctgatcaac gacagattcg
     3781 gggaagggga atgaaccagg ggcgaggggc gaggggcgag gaacgagtca aaaccgcgca
     3841 gcctaaatca acgtttcact tactcgcccc tcgcccctcg cccctcgccc cagctcgcgc
     3901 atgagcttca ccatccacgg catcggggta tcggggggca tcgccatcgg ccatgcccaa
     3961 ctggtatcgc acgcccagct cgaagtcgcg cattacaaca tctccgagtc gcgggtcgcg
     4021 gatgagcagg cccgcttcga cgcggcactg gtaacggcgc gcgctgaact tgccagcctc
     4081 ggccagcacg ttccggccgg cgcgccggcg gagttcgaag cctttctcaa tctgcatctg
     4141 atgatcctcg acgatccgac gctatcagag gtgccgaaac agctcatcga gacccagcag
     4201 tgcaacgcgg aatgggcgtt gaagctgcag atggaagcgc tgatggatca gttcgagcgc
     4261 atcgaggacg gttatatacg cgagcgcaag accgatgtca tccaggtcgt ggaacgggta
     4321 ctgaaggtcc tgatggggcg gcccggcgaa gtcctcgcgc acagcgcgcc cgaggagaac
     4381 agcatcctgg ttgcgcacga cctctcgccg gcggatgtca tcctcttcaa gagccacaag
     4441 ttcgccagtt tcgtcacaga ccttggcggc gccacgtcgc acaccgcgat catcgcccgc
     4501 agcctgaaca tcccgtccgt ggtggccctg caccacgcac gcacactgat ccgcgagaac
     4561 gaagtgctga tcgtggacgg caccaacggc gtggtcatca tcaatcccga caggaccgtg
     4621 ctgggcgagt acaaactgcg ccaggaacag tgggagctcg aacgcctgaa actcaggcgg
     4681 ct
//
LOCUS       DASQOP010000115         3163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2262866, whole genome shotgun sequence.
ACCESSION   DASQOP010000115 DASQOP010000000
VERSION     DASQOP010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2262866"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VE030_04510"
     CDS             <1..209
                     /locus_tag="VE030_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HZF22715.1"
                     /translation="RMSAEPPLSYLVVYTPPSREFVCVEPVSNTVDWLNLGHLETAQI
                     GGMVLSPGATITGRCRFEPDFGAT"
     gene            complement(388..1158)
                     /locus_tag="VE030_04515"
     CDS             complement(388..1158)
                     /locus_tag="VE030_04515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZF22716.1"
                     /translation="MTQDRLAIYPSLKNKVVFITGGGSGIGASVVEGFVRQGAKVGFV
                     DIDESASKELVRKLEGTGRAPRFIRCDVRDIGQLRAAIKATQSESGDIGVLVNNAARD
                     DRHSIESVTVEFWDERIAVNLRHQFFASQAVVEQMKRLGGGSIINFGSISWMTATGGM
                     PAYTTSKAAVHGLTRGLARDLGPFNIRANTVSPGWVMTERQKKLWVTPEGEKQMDALQ
                     CLKGRLQPEDLANMVLFLASEDAKMITSQNFVVDAGWT"
     gene            1451..2710
                     /locus_tag="VE030_04520"
     CDS             1451..2710
                     /locus_tag="VE030_04520"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZF22717.1"
                     /translation="MYTNRREFLKAVAALLAAAAAQQGRFAYAGDADLQRIADALLRQ
                     AAEAGDVPGVIATATTRDSTIYEGAFGSRVLGQDAPMTMDTIVWIASMTKALTGTAAM
                     QLVEQGKLELDSPASRWVPELADAKVLEGWANGQPVTRPPKRPITLRHLLTHTAGFSY
                     ELWSTDIQQYQKAMGIPGIISCQNAALRTPLLFDPGERWDYGINIDWAGKMVEAVSGK
                     RLGDYLRENLFNPLGMNSTAFKITPEMRARLAKIHQRGADGAFTPTEIEIPQEPEFQM
                     GGGGLYSTAGDYLKFVRMILNRGKGNGNQVLKPDTVDLMSRNAMGNIKVTMLKTAIPA
                     LTNDAEFFPGMPKSWGLSFMINEQTAPTGRSAGSLAWAGLANSYFWIDRNKGVGGVYL
                     TQVLPFADEKALPLFYAFEKSVYQSLG"
BASE COUNT          611 a         1006 c          954 g          592 t
ORIGIN      
        1 tgcgcatgag cgccgaaccg ccgttgtcct acctggtggt ctacaccccg ccgtcgcgcg
       61 agttcgtgtg tgtcgagccg gtcagcaata cggtggactg gctgaatctc gggcatctcg
      121 aaaccgcgca gattggcggc atggtgctat cgcccggcgc gacgatcacg ggccgctgcc
      181 gcttcgaacc cgatttcgga gcaacctaac gggtgaatga gggaagggtg ggaaaggcgt
      241 gaaggtgtga aggagtgaat gtgtgaatga gtaagagcgg cttcgccttc acgtcgtatc
      301 aatcttcagg cttttgccct tcactcattc actcgttcac tcattcacac cttcacgcct
      361 ttcccaccct tcccccttca ccgctcttca cgtccagccc gcatccacga cgaaattctg
      421 cgacgtaatc atcttcgcgt cctccgacgc aaggaacagc accatgttgg caaggtcttc
      481 gggctgcaac cggcctttca ggcactggag cgcgtccatc tgcttctcgc cctccggcgt
      541 cacccagagt ttcttctgcc gctcggtcat gacccagcct ggggacactg tatttgcgcg
      601 gatgttgaac ggtccgaggt cgcgcgccag tccgcgcgtc aggccgtgga ccgcggcttt
      661 gctggtggtg taagccggca tgccgccggt tgcggtcatc caggaaatcg atccgaagtt
      721 gatgatcgat ccgccgccga ggcgcttcat ctgctcgacg acggcctgcg acgcgaagaa
      781 ctgatggcgc aggttgaccg caatgcgctc atcccagaat tcgaccgtca cgctctcgat
      841 cgaatggcga tcgtcgcgag cggcgttgtt caccagtaca ccgatgtcgc cggattccga
      901 ctgcgtcgct ttgattgccg cgcgcaactg cccgatatcg cgcacatcgc agcggatgaa
      961 gcgcggtgcc cgcccggtgc cttcgagctt ccgaacaagc tctttgctgg cggattcgtc
     1021 gatgtccacg aacccgacct tcgcgccctg gcgcacaaat ccctcgacga cggaggcgcc
     1081 gatccccgat ccacctccgg tgatgaaaac aaccttgttc ttgaggctgg ggtagatggc
     1141 gagacgatcc tgtgtcatga ctgtttccgg cagcgaatga aaaaataggc cggtcacggc
     1201 cggcaggttt gacctcacca gtatatccgg gagacttgcc cgaaagcagg aggcctgccg
     1261 ggctgcgcag tgccgccgtt ccctgccccg ggcactgagc acctgccgat cttcatcctt
     1321 catcccgcac ggcaatcatg ccttgtccgg cggcaagcaa caggtctatt cttcctttcg
     1381 cggccgcgtc atggtgattg ccagtcgtgc gacaaggccg acgaactcaa aaatcacagg
     1441 gaggaaacgt atgtatacca accgtcggga gttcttgaag gccgtggccg ccctgctggc
     1501 cgctgcggca gcgcagcagg gccgctttgc ttacgctggc gacgccgatc tgcagcggat
     1561 cgccgatgcg ctgctgcggc aggcggcgga agctggcgac gtcccgggcg tgatcgccac
     1621 ggctactacc cgcgacagca ccatctacga aggggcgttc ggcagccgcg tgctcggcca
     1681 ggacgcaccg atgacgatgg ataccatcgt ctggatcgcg tcgatgacca aggcgctgac
     1741 cggcaccgcg gcgatgcagc tggtcgaaca gggcaagctc gaactcgaca gccccgcgag
     1801 ccgctgggtg ccggagctgg ccgacgcaaa agtactggaa ggctgggcca acggccagcc
     1861 ggtcacccgc cccccgaagc ggccgatcac gctgcgccat ttgctcacgc acaccgcggg
     1921 cttttcctac gaactctgga gcacggacat ccagcaatac cagaaagcca tgggcattcc
     1981 gggaatcatc tcgtgccaga acgccgcgct gcgtacgccg ttgctgttcg acccgggcga
     2041 gcgctgggat tacggtatca acatcgactg ggccggaaag atggtcgagg cggtgagcgg
     2101 caagcggctc ggcgactatc ttcgggagaa cctcttcaac ccgctcggca tgaacagcac
     2161 ggcgttcaag atcacgcccg agatgcgggc gcgcctggcg aagatccatc agcgcggcgc
     2221 agacggcgcg ttcaccccga cggagatcga gattccgcag gagccggagt tccagatggg
     2281 cggcgggggt ctctactcga ccgcgggcga ttacctgaag ttcgtgcgca tgatcctcaa
     2341 tcgcggcaag ggcaacggca accaggtgct aaagcccgat acggtggacc tcatgtcgcg
     2401 caacgccatg ggcaatatca aggtcacgat gctcaagacc gcgatcccag cgctgaccaa
     2461 cgacgcggag ttcttccccg gcatgccgaa gagttggggc ttgagtttca tgatcaacga
     2521 gcagaccgcc cccacgggcc gctcggccgg cagccttgcc tgggccgggc tggcgaacag
     2581 ctacttctgg atcgaccgta acaagggtgt cggaggcgtg tacctgaccc aggttctgcc
     2641 gttcgccgac gagaaggcat tgccgctctt ctacgccttc gagaagagcg tctatcagtc
     2701 gctcggctga gacagccttt gcgatgcccg agcacgcgct gcggagttgc cgcggcgcgt
     2761 cgttgggaaa aaggggaatg gggaatggga aattaatgca gcgaattgac ggttggcgat
     2821 ttcactttca attttcgcct ttcacctttc atctttgagc tttcaccttt tgtgcgcact
     2881 gcactcagaa ctcagtactt agcactcagc accggcctta tcagtcaccg cattccccgc
     2941 cgtcgccgac cggagacacg ccgatcggag gaaggtctct tgcgaactcg atgcggcaac
     3001 gcgccactaa atcgatgacg cggcctccag tgtccccacc cgcgcgcgcc aacacatggc
     3061 gcgagtgtcc aaacgcgcgg gatcgactcg ggtcagacag caggaatccg acacgcacgc
     3121 tcaagaactg aattcaggaa gtttcttgcg tacgccgcga gct
//
LOCUS       DASQOP010000116        19672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2467443, whole genome shotgun sequence.
ACCESSION   DASQOP010000116 DASQOP010000000
VERSION     DASQOP010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 19672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19672
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2467443"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..639
                     /locus_tag="VE030_04525"
     CDS             211..639
                     /locus_tag="VE030_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004553866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HZF22718.1"
                     /translation="MFNPSREDVRRFFFETWRKYRTQAPLEGLEKTALEVALLHPEYH
                     ALLDSEDRNVDRDFLPEGGQLNPFLHLSLHLALAEQISIDQPRGIRGLHAQLLTHAQS
                     DHDALHLLLECLGETLWRAQRDHTAPDEEAYLDCIRRKLG"
     gene            complement(1038..1367)
                     /locus_tag="VE030_04530"
     CDS             complement(1038..1367)
                     /locus_tag="VE030_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZF22719.1"
                     /translation="MNKLLIAVIVGAGFAVAGGAVAADAAAGKEKSQTCAACHGPDGN
                     TPMTPEFPKLAGQPADYLEKALRDYKKGARKNPMMMPMAQGLSKQDIKDLAAYYSSLK
                     GDLKVKY"
     gene            complement(1395..1727)
                     /locus_tag="VE030_04535"
     CDS             complement(1395..1727)
                     /locus_tag="VE030_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZF22720.1"
                     /translation="MKFRSLAIAGLLAVLASVAAPLLAEGDPAAGKNKTSMCAGCHGI
                     PGFRAAYPETYHVPKLGGQNPGYIVAALKAYKAGERSHPTMKAIASSLSEQDMADLAA
                     YYADAGRK"
     gene            complement(1836..2672)
                     /locus_tag="VE030_04540"
     CDS             complement(1836..2672)
                     /locus_tag="VE030_04540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated SoxYZ-like
                     carrier"
                     /protein_id="HZF22721.1"
                     /translation="MRYGTHRVGTLLAALVFAVPVLANEKAEPDPARSPYWEPIRQLM
                     FGDRPINADDRDVIRVYLNMRADDASTVPVAVKSQIDQTEKDFIKSIYLIVERNPSPT
                     AGVFHFTPSSGRAQVETRLRFEDFSFVRAIAEMNDGKLYMSQRWVKAAGGCSAPNARN
                     FVPEALLGKMKLRFDDDSITYGKPNLVQLMIRHPNESALAADFDAEKVPQFVRSVKVS
                     FDGRPVMDAEVDFSISDNPNFRFFFNPQGKGELKIEVEDTHDRKFTHSVPISEGAPLP
                     GG"
     gene            complement(2747..3691)
                     /locus_tag="VE030_04545"
     CDS             complement(2747..3691)
                     /locus_tag="VE030_04545"
                     /inference="COORDINATES: protein motif:HMM:TIGR04559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein relay system zinc metallohydrolase
                     2"
                     /protein_id="HZF22722.1"
                     /translation="MKRIKVTAAMLGAVAVVAAQTAMAVDPAQIGSFEVTEVAPGNYV
                     HYGSFEERSPQNLGDNANIGFIVGERCVLVVDAGGSLPVGRALKKAIRRVTQVPICHV
                     VLTHVHPDHFFGAAAFLDGGAQFIAHRNYPRQLAARARSYLNYLRRDLGELAEGSDVV
                     QPTLLIDGRLELDLGGRSVVVQGWPPAHTDDDLTVFDRATRTFWLADLLFVGHTPVID
                     ATITGFLAVMDDLRKIDAAHYVAGHGRSQAPWPEVLDPQRRYFTVILEETRAAIRNRK
                     TLQQATDEVGHSEAKNWAAFDTFHRRNVTTAYTELEWE"
     gene            3799..4767
                     /locus_tag="VE030_04550"
     CDS             3799..4767
                     /locus_tag="VE030_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZF22723.1"
                     /translation="MISVPANAVRLATIALLLACPARAADELRPEKVADGVYAFVADT
                     GEISAANRGHVGNSGFIVGPTGVVVIDTGVSYRHGRRMLTAIGRVTAKPVELVVVTHA
                     VQEFLFGNAAFEERGVAILAHSETAALMKARCAHCLDNLRPVLGDELEGTRLVLPRRQ
                     VSDTTTIEAGGRQLELLYLGWASTPGDLAVFDRTTGTLFAGGLVAVNRIPDIRDCDFE
                     GWTRALEALRELPVKRVVPGHGAVSGAEAIRSTTDYLHALDEKINALYARSSSLLESV
                     DNAALPAYGAWAMYATTHRQNALHRYLQLEIEELGGDPRSTALPQR"
     gene            4853..5416
                     /locus_tag="VE030_04555"
     CDS             4853..5416
                     /locus_tag="VE030_04555"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011544630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HZF22724.1"
                     /translation="MGHRLTKIYTRTGDAGTTGLGDGSRVSKDSLRIEAIGAVDELNS
                     VIGVLLAETPADKVHQCLHDVQHDLFDLGGELSIPGYTSVGDTHVARLERLLDGFNAD
                     LPALKEFILPGGSRAAALCHVARTVCRRAERRVVALAAAEQLSPALQRYLNRLSDLLF
                     VLCRVLNRDAGVTDVYWQKGKNRSEPA"
     gene            5422..5979
                     /locus_tag="VE030_04560"
     CDS             5422..5979
                     /locus_tag="VE030_04560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZF22725.1"
                     /translation="MDAEQLSGLLSRCVLRDQQAFRTLYQHSSAKLFAVAVRITRRRD
                     WAEEVLQESFINIWNHASGYNAAKSAPMTWMTAIVRNRALDLLRRPREVEIDEGHEDL
                     MASIPDESPGPEELLKQSMEAGALAECMKQLTGEQQKSIALAFFNGLSHGELARQMQK
                     PLGTVKTWIRRGLDRLKSCLDAHGI"
     gene            6019..6735
                     /locus_tag="VE030_04565"
     CDS             6019..6735
                     /locus_tag="VE030_04565"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HZF22726.1"
                     /translation="MRFASEELRSKLAAEYVLGTMSAHARRRFEINLKEHPALRRAVA
                     EWEKRLMPLALTLPEVEPPSRVWQAIESRIRPGRQGRPGFWENLSFWRFSSIASGLVA
                     LALLILVVVPGPEAPPADGTRMVVVMNDLKTRSPAMTVSWEPGMPGKRVMRIRVIGHA
                     EMAPGTAWELWMLPGKDQNPVSLGLITTHDAQTVIVPEPLATKLDQAQGLAMSVEAAG
                     GSPTGLPTGPVLYAGQCVKT"
     gene            complement(6975..7850)
                     /locus_tag="VE030_04570"
     CDS             complement(6975..7850)
                     /locus_tag="VE030_04570"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HZF22727.1"
                     /translation="MRRLFFFLNLVFQFFAVTAAAAVEVIDDRGVVLRRDWPAQRVIA
                     LAPHLTELVFAVGAGNRLVGKVRGSDFPPGARALAEVGDAAGLDFERILALRPDLVLA
                     WGSGNRVADVERLERLGFAVLVLEPRRLEDIPRHMRMLGVLLGSGRRAQAAALAFDSH
                     AERLRERYRGKSAVSVLFEAWHHPLITVNAEHLISDVLRLCGARNAFGDLSQLAAVVS
                     PEQVLAADPDAIIIGSEAEDAGSQGWQRYPYLKSVRMGAIFTVSADLITRQTPRVLDA
                     AEKICADLDTLRTRP"
     gene            complement(7866..8366)
                     /locus_tag="VE030_04575"
     CDS             complement(7866..8366)
                     /locus_tag="VE030_04575"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HZF22728.1"
                     /translation="MLLNTLEHLGEGTALAQQIFDMIGHSKFFADFTREDVEKLTGFM
                     QIYRAEAGQMIIREGDIDDYMLLIIQGRVEIVKTDSHGHLQPMTTVGSGATLGEMSMI
                     DGEPRFATCMGLEPTTFAVLSRDSMVQIILEEPSLGAKVLVKLVTLLSQRLRQTSSNL
                     LHYMER"
     gene            complement(8529..9656)
                     /locus_tag="VE030_04580"
     CDS             complement(8529..9656)
                     /locus_tag="VE030_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22729.1"
                     /translation="MILILSDSADPQSPEYEQLMQALALLPNIKTRIHRIQGTERSVT
                     EVYLLGDTKALAIEDMQSLPCVERVIRVSEEYRVLGRHPGDTRPTHFDYQGLRFGQDT
                     LHVFAGLCAVDSREHVEIMMRALKDNGQQCTRMGAYKPRTSPYAFQGHGKACLPYVFE
                     LAGKYGIKVIAMEVTHESHVEEIRQAMRDTGNPTGVMLQIGTRNTQNFELLKIVGRQK
                     EFPVLIKRGFGITLDESLNAAEYLASEGNHKVLFGLRGMKTNLGDPHRNFVDFSHVPA
                     VKRLTRMPVCVDPSHSVGSRMAGPDGILDVMHVTAQGVLAGANMVLVDFHPNPAKALV
                     DGPQALRLDELPHFLADVAIAREACVKRTELARRYRDTATS"
     gene            complement(9810..11270)
                     /gene="tldD"
                     /locus_tag="VE030_04585"
     CDS             complement(9810..11270)
                     /gene="tldD"
                     /locus_tag="VE030_04585"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HZF22730.1"
                     /translation="MPNDPTNNHELLFATADSTLLAPYGLDAGGLARVFGQIMSHSVD
                     YADLYFQYSRSEGWSLEEGMVKSGSFNIDQGVGVRAVSGEKTAFAYSDDISVPALESA
                     AYATRAIARQGGVGTTRVAKRTQGRELYLPQDPLGSLSDQDKVKLLEQLERHARALDS
                     RVVQVMATLAGEYEVILVARSDGVLAADVRPLTRLSLQVIVEENGRREQGSAGGGGRF
                     DYAYFSDDILREYAAKAVHQAATNLSARPAPAGTMTVVLGPGWPGVLLHEAIGHGLEG
                     DFNRKGSSAFSGRIGERVAAAGVTVVDDGTIARRRGSLNVDDEGNPTQRNVLIEDGIL
                     MGYMQDALNARLMGASPTGNGRRESYAHITMPRMTNTYMLNGDKDPQEIIASVKHGLY
                     AVNFGGGQVDIVSGKFVFSAAEAYMIENGKVTYPVKGATLIGNGPDALTRVTMIGNDM
                     SLDSGIGTCGKEGQSVPVGVGQPTLRIDGLTVGGTA"
     gene            complement(11460..12359)
                     /locus_tag="VE030_04590"
     CDS             complement(11460..12359)
                     /locus_tag="VE030_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HZF22731.1"
                     /translation="MATINRSGFGRAKGRGAREHSPAGASKNPPHGFRIAGVQMASGP
                     NVSANLNEAGRLIEMAVAEGAKLVALPEYFAIMGMRPTDKVKVREKDGDGPIQAFLAE
                     TAARLGIWLVGCSVPMETPVEGKIYNSCQVYDDTGKMVARYDKIHLFSLDLGKEKFAE
                     QGTIEPGKQVKVVDSPFGRIGLSVCYDLRFPELYRAMKDVDIIVVPSAFTETTGRAHW
                     EALVRARAIENLSYVLAPAQGGYHLSGRETHGDSMIVDPWGTVLDRLPRGSGIVSAWI
                     NPAYQASLRKSLPALTHRAIHRI"
     gene            complement(12415..16227)
                     /locus_tag="VE030_04595"
     CDS             complement(12415..16227)
                     /locus_tag="VE030_04595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HZF22732.1"
                     /translation="MVLKHSLFILKHGSLWTYRLATYAVLAAGIVFVALVIGLRYVVL
                     PNINDYREAIAQAVSRAAGQRVSIGAITGSWQGYQPELSFQDVRVFDTRDQQALSLGR
                     VEAVLSWLSLLDAEVRFESLEIVEPELEVRRDAAGELWLAGIAVERSQGRGGGFADWL
                     LTQRQVLVRSAQIVWRDETRAAPDLRLTSVNIRLDSDGESHRFGLNASPPAEVASQIV
                     ARGELWGRSAHDLQAWRGRLYLEFGYADLAQAKTWIAVPFDVARGLGSLHVWMDLEGP
                     GVAAATADLRLVNVQARLAPDLPELALANLQGRLAWNSRGGRTQISAQALGFTTADGL
                     TLAPMQLSFTRTGTGDGGGHSELRLEKLDLAPVVELAEYLPLDASLRGRLGRSSPSGM
                     VDEALFSWEGEWNAERPYRAHARFSGLTLKPDGVFPGFRGINGQFDADQGGGTVSLSA
                     RDAHVELPRVFSEEITLDVLTAEAGWKFQDGHTLVTLKNVAFTNAHLAGNVYGWYRTD
                     PEGDRGSADLAGSMVRVDARQLWRYIPVFAPVTQAWLRRALLAGESRDVRFRLQGPLN
                     DFPFENDKTGLFEVKTRVTGVTLDYADGWPPLTNAVADVVFRGRRMEVRPQAGSILGL
                     RLSDVLASIPELGKHDEHLLVKGVAEGPTAEFLRFVESSPVSRHINRFTEDMKASGNA
                     RLDVELDLPLRQIADSVISGVLVLRDNQITVDPRLPPLEGFGARMAFTKKTVSVKDGR
                     ARLAGNPISFEASNQKDGGMVLNVAGTLDATRLRSVSNHPALQFVEGQTEWRGNIGVR
                     DKMASLRIDSSLVGIGSTLPAPFAKAAGTSLPLHLELRERPGRQRLLAIDLGKVASAR
                     VLLDSADPDGIKRGMVSLGATASLPAADGLWVNGSLDVVDVDSWRPIFAGGSGSPSVD
                     LAGADLQIGILDAHRRRFHDLRVNATRQDSAWQAALSGREIAGQLSWASAGEGRLSAR
                     LTRLTLPPLTTTLETGAADSFSSERRPSVDLVAESFTFEGKDLGQLTVLADPEPSGWR
                     LQRLEITNPESKLSINGRWAVVVVSRTDVEVRLEVNDIGKFFSRVGWHEGVKGGSALL
                     EGPVAWAGSPYRVDIPSLSGQLKLEAKNGRFRQIEPGVAKLLGILSLQALPRRASLDF
                     QDVFSKGFTFDRISANLSIANGVVHTEDFMMEGSAARVGMRGRVDLAAETQNLIVRVT
                     PSLSESIAVAGAIVNPAVGVAAYLAQKALKDPLSKIASFEYSVTGTWADPVVARINKP
                     VESPPSRR"
     gene            16447..19152
                     /gene="glnE"
                     /locus_tag="VE030_04600"
     CDS             16447..19152
                     /gene="glnE"
                     /locus_tag="VE030_04600"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HZF22733.1"
                     /translation="MSSPALSNGTQSESIARAARLSRYARHLLSANPALAAEAAVDLP
                     FAPDEMRARLSGADFGDEDALKTGLRRLRQAVMLRLITRDLGGRADLAEVVATATALA
                     EESLRTAAARLAQWLTRDFGRPVGGASGDVQELHVIGMGKLGGAELNVSSDIDLIFAY
                     PEDGETDGARRISNNEYFTRLARKLIAAIGEVTADGFVFRVDTRLRPYGDSGPLVASF
                     DMLEDYLATQGREWERYAWVKGRTISGDRGAQLEAIVAPFVYRRHLDFSALASLRELQ
                     AQIRQEVQRREIADNIKIGRGGIREIEFLVQVFQIIRGGREPQLRLRPTLKALALLGE
                     KQLLPAAAVGELQAAYLFLRNLEHRLQYLDDQQTQMLPAGAEDRQLVAEMMGYPAYAD
                     FDRDLAGHRGVVSRHFEAIFAGQREEATADPCADLWLQQLPEKDALARIAALGYADPA
                     RILLLISDIRSSTRYRRMAASGQARLDQLMPKLLKLATTLPGPDATFERVLQVVESIG
                     RRESYLALLLEYPLALARLARLASASPWAAGYMAQHPILLDELITPQSGQAPDWPQLR
                     SALRASLDEHAGNTERQMDILRHFKQTQTLRLLALDLGGNLPLETLSDHLSDLACVVL
                     EEVLRLAWDGLRVRHCDQPRFAIVGYGKLGGKELGYASDLDIIFLYDDAHESAPEIYA
                     RLAQRINTLLTSTTSAGVLYETDLRLRPNGVAGLLVSHIDAFRDYQFKDAWIWEHQAL
                     TRARAVAGDAQIGARFEANRIDLLRRRRDLTLLSQEIRAMRDKMRQSHPNDTRLFDLK
                     HDRGGIVDVEFMVQYLVLGEAHHHAELTGNIGNLALLKLAARLGLIPEDLAAAAHAAY
                     RQFRRLQHQLRLQGERYARVDPATVAEHAQAVVKLWDKVFSEQPA"
     gene            complement(19408..>19672)
                     /locus_tag="VE030_04605"
     CDS             complement(19408..>19672)
                     /locus_tag="VE030_04605"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZF22734.1"
                     /translation="QLHIGIATGPAIAGVIGATRFIYDLWGDTVNLSSRLTAEAAGGI
                     ILVDKTTYRRLGHRYSFEEPRTISIKGKGETTVYRLTGKLYSG"
BASE COUNT         3637 a         6394 c         6034 g         3607 t
ORIGIN      
        1 aggatgaagg atgaaggatg aaggatgaag gatgaaggat tggacctctc gcctggagag
       61 atgcgctgct tctgtccacc ccagagcccg gctccacggt caattgatcc ccacaatttg
      121 atcctggccg gctatgctac accccgcctt gagcgtcgat catcctgcca agcacactcg
      181 cttccgcctt catccttcat acttgctttc atgtttaatc cgtcgcgcga agacgtgcgc
      241 cgtttctttt tcgaaacctg gcgcaaatac cgcacgcagg cccctttgga aggattggag
      301 aaaaccgcgc tcgaagtggc gctgctgcac cccgagtatc acgcgctgct ggatagcgaa
      361 gaccgcaatg tcgaccgcga tttccttccc gaaggcggtc aactcaatcc cttcctgcac
      421 ctgtcgctgc atctcgctct cgccgagcag atctcgatcg atcagccgcg cggaatccgc
      481 gggctgcacg cgcagcttct tacgcacgca cagtcggacc acgatgcgct gcacctgctg
      541 ctggaatgtc tgggggaaac gctatggcgg gcacagcgcg atcacacggc gccggatgaa
      601 gaggcttatc tggactgcat tcgccgcaag ctaggatgag cggcacgctc tatcagtcaa
      661 aaaaaggatt gttcggacat tcttgtgggc aataggtgac cggcagctgg aggattcacc
      721 ggctggcgaa agaacacgct cgtcaccaaa cccgtactgc caagcgttaa ccgcaaggga
      781 cgccaaggac gcccgggaaa ggcaaatgca aaaagcaggg ggttttgaac gctgacgcaa
      841 gtatctgagc ttcgaccaac ccgcgccgct tacggttacc ctcgccttcc tgccctttcc
      901 tttccctggc gtccttggcg tccttggcgg tgaatgcgta gttggtgaat catttgaggc
      961 ccaaagataa gtatgatggc ccaaaaaaaa tgaggagcga tcggctcctc ttttttcttc
     1021 acgcttacgc atccaactca gtacttgacc ttcagatcgc ccttcaggct ggagtagtag
     1081 gcggccagat ccttgatgtc ctgcttggaa aggccctgcg ccatgggcat catcatcggg
     1141 ttcttgcgcg cgcccttctt gtaatcacgc aacgcctttt ccaggtaatc ggccggctgt
     1201 ccggccagct tgggaaactc cggcgtcatc ggcgtattgc cgtccgggcc gtggcaggcc
     1261 gcgcacgtct gggatttctc cttgcccgcc gccgcatccg cggcaacggc gccaccggcg
     1321 acggcgaaac ctgctccgac aatgaccgca atcagtagct tgttcatatc ttccttttcg
     1381 cttcttcggc cggcttactt cctgccggca tcggcgtagt acgcagccag atcggccatg
     1441 tcctgctcac taaggctgga agcgattgcc ttcatggtcg gatggctgcg ctcgcccgct
     1501 ttgtaggcct tcagcgcggc cacgatataa ccgggattct gtccgccaag cttgggaacg
     1561 tggtaggtct cgggataagc ggcgcgaaac ccgggaatgc cgtggcaacc ggcacacatc
     1621 gaagttttgt tcttgcctgc cgcgggatcc ccttcggcga gcagtggtgc ggccacggac
     1681 gccaacacgg cgagcagacc ggcaatggcg aggctgcgga atttcatgtc ttggagtttt
     1741 cctggttttg gcaataaaaa aaccgcgcaa ctttagccga agcggatacc ccggtcaacg
     1801 ctgatgcggc ccttggaacg accggaaccc ggggatcagc ccccgggcag aggagccccc
     1861 tcggatatcg gcaccgaatg ggtgaatttg cggtcgtggg tatcttccac ctcgattttc
     1921 aactcgccct tgccctgcgg attgaagaag aaccggaagt tgggattgtc cgatatcgag
     1981 aaatccacct ctgcatccat caccggcctg ccgtcgaacg aaaccttcac gctgcgcacg
     2041 aactgcggca ctttttccgc atcgaagtcc gctgccagcg cggactcatt ggggtggcgg
     2101 atcatcagtt gcaccagatt gggcttgcca taggtgatgg aatcgtcatc gaaacgcagc
     2161 ttcattttcc cgagcaacgc ctccggcacg aagttcctgg cgttgggcgc cgaacagccg
     2221 ccggccgcct tcacccagcg ctgcgacatg tagagctttc cgtcgttcat ttccgcgatc
     2281 gcgcgcacga agctgaaatc ttcgaagcgc aggcgagtct cgacctgggc gcggccgctc
     2341 gatggagtga agtgaaacac gcctgccgtc ggagaggggt tgcgttcgac gatcaggtat
     2401 atcgacttga tgaagtcctt ttcggtctga tcgatctgcg acttgacggc taccggcacg
     2461 gtcgaggcat cgtcggctcg catgttgaga tagacgcgga tcacgtcccg gtcgtcggca
     2521 ttgatcggcc ggtcgccgaa catcagttgc ctgatcggct cccagtaggg gctacgggcc
     2581 ggatcgggtt cggccttttc gttcgcgagc acaggcacgg cgaacaccaa ggccgccaat
     2641 aacgtgccaa cccgatgcgt gccgtatctc atccgcgccc tctcttaaat tctgcctggc
     2701 aattccgtgg gaagtataac ccagtccccc aagaaatcag gcgccctcat tcccattcga
     2761 gctcggtata cgccgtggtc acgttgcggc ggtggaaggt gtcgaaggcg gcccagttct
     2821 tcgcctcgga atggccaact tcgtcagtag cctgctgaag cgtttttctg ttccggatcg
     2881 cggcgcgcgt ttcctcgagg atcaccgtga aatagcgccg ctgcggatcg agaacctctg
     2941 gccaaggtgc ctgcgagcgt ccgtgccccg caacgtagtg cgccgcatcg atcttgcgca
     3001 ggtcgtccat cacggcgaga aagccggtaa tggtggcgtc gatcaccggc gtatggccca
     3061 cgaacagcaa gtccgccagc caaaacgtgc gggttgcgcg gtcgaacacc gtgagatcgt
     3121 cgtcggtatg cgccggcggc cacccctgca ccaccacgga tcggccgccg agatcgagtt
     3181 cgagcctgcc gtcgatcagc agcgtcggct gtacgacgtc gctgccctcg gccaattcgc
     3241 ccagatcacg tcgcaaataa ttgagatagg atcgcgcgcg agcggcgagt tgccgcggat
     3301 agttccggtg ggcgatgaat tgcgccccgc cgtcgaggaa agccgccgcg ccaaaaaaat
     3361 ggtccgggtg cacgtgagtc agcaccacgt ggcatatcgg cacctgcgtc acccgccgga
     3421 tcgctttttt cagtgcccgg ccgaccggaa gcgatcctcc cgcatccacc accagcacgc
     3481 atcgctctcc gacgatgaaa ccgatgttgg cattgtcgcc caggttctgc ggggaccgct
     3541 cctcgaaact gccgtaatgc acgtagttcc cgggcgcgac ttcggtcact tcgaagctcc
     3601 cgatctgcgc cggatctaca gccattgcag tctgcgcggc cacgacggcc accgcaccca
     3661 gcatggcggc ggtcaccttg atccgtttca cgcgttgtcc ccgtgtgtgc accggcccat
     3721 tagaacagaa cccttttgcg cttgcccgcg ctgcgggtct cgattacggt gagcacttta
     3781 ctcagggctt gcttgcacat gatttcagtt ccggccaacg cggtgcggct ggcgacgatt
     3841 gcgctgctgc tcgcctgccc ggcccgcgcc gcagacgaac tcaggccgga aaaggttgcc
     3901 gatggtgtgt acgcattcgt cgccgacacc ggcgagatct ccgcggcaaa tcgcggccac
     3961 gtgggcaaca gcggcttcat cgtcgggccc accggcgtgg tggtcatcga caccggcgtc
     4021 tcataccgcc acggccgacg catgctgacg gcaatcggcc gggtgacggc caagccggtc
     4081 gaactggtcg tggtcacgca cgctgtgcag gaattcctgt tcggcaacgc cgcattcgaa
     4141 gaacgcggcg ttgccatcct cgcgcacagc gaaactgccg cgctgatgaa agcgcgctgt
     4201 gcacattgcc tggataacct gcgcccggtt ctcggcgatg agctcgaggg cacccgcctg
     4261 gtgctacctc ggcggcaggt gagcgacacc acgacgatcg aggccggcgg gcggcagctc
     4321 gagctgcttt acctgggctg ggcgagcaca cctggcgacc tggctgtgtt cgaccgcacc
     4381 actggcacct tgttcgccgg cggcctggtc gcggtaaacc gcattccgga catccgcgac
     4441 tgcgatttcg aaggctggac ccgcgcgctc gaagcgctgc gggaactgcc ggtgaagcgc
     4501 gtcgtgccag ggcacggcgc cgtgtccggt gccgaggcga tccggtcaac tacggattac
     4561 ctgcacgccc tggatgagaa aatcaacgct ctgtacgctc ggagttcgag cctgctcgaa
     4621 tcggtggata acgcggcgct gccggcctac ggcgcctggg ccatgtacgc cacgacccac
     4681 cggcagaacg cgctgcatcg ctacctgcaa ctggagatcg aggaactcgg tggcgacccc
     4741 cgctcgactg cactaccgca gcgctgaaat ccccggatta ctgaatccgg attccgggcc
     4801 gcaacgtatc gatcccctat aatgcggcga acagccgctc ttcctggaac acatgggtca
     4861 ccgcctgacc aagatataca cccgcaccgg cgatgccggc accaccggac tcggcgacgg
     4921 ttcgcgcgta tccaaggaca gtctgcgcat cgaggcaatc ggcgcggtgg acgaactgaa
     4981 cagcgtcatc ggagtactgc tcgccgagac gccggcggac aaggtgcatc aatgcctgca
     5041 cgacgtgcag catgacttgt tcgatctcgg cggcgaactc agcatcccgg gatataccag
     5101 cgtcggcgat acgcacgtag cgcggctgga gcgcctgctt gacggtttca acgccgatct
     5161 gcccgcgctc aaggaattca tcctgccggg cggttcgcgc gccgctgcac tgtgccatgt
     5221 ggcgcgcacc gtttgccgcc gcgccgaacg ccgagtggtt gctctcgccg ccgcggaaca
     5281 gttgtccccg gctttgcagc gctatctcaa ccggctctcc gatctgctgt ttgtgctttg
     5341 ccgggtactc aaccgcgacg ccggcgtgac cgacgtgtac tggcaaaagg gcaagaaccg
     5401 cagtgagccc gcgtgacagc catggatgcg gaacaactct ccggcctgct ttcccgctgc
     5461 gtcctgcgcg atcagcaggc cttcaggacg ctgtatcagc acagctcggc gaaactgttt
     5521 gccgttgcgg tacgtattac ccgtagaagg gactgggccg aagaagtact gcaggagagt
     5581 ttcatcaata tctggaacca tgcctcgggt tacaacgcgg cgaaaagtgc accgatgacc
     5641 tggatgaccg ctatcgtgcg caaccgggcg ctggacctgc tgcgccggcc gcgggaagtc
     5701 gaaatcgacg aaggccacga ggacctgatg gcatcgatcc cggatgagtc ccccggcccg
     5761 gaggaactgc tcaagcagtc gatggaggcc ggcgcgctgg cggagtgcat gaagcagctg
     5821 accggcgagc agcagaaaag cattgcgctg gctttcttca acggtctttc tcacggggaa
     5881 cttgcgcggc agatgcagaa accgctcggc accgtgaaga cctggattcg ccgcgggctg
     5941 gatcgcctga aaagctgcct tgacgcacat ggcatatgac gcaatcctgc tgacaacccg
     6001 gtacatcggg tcgcgaacat gagattcgcc agcgaagagc tgcgcagcaa actggctgcc
     6061 gagtacgtgc tcggcaccat gtccgcgcac gctcgccggc gtttcgaaat caacctgaag
     6121 gagcatccgg ctttgcggcg cgcggtggca gagtgggaga aacgcctgat gccactggcg
     6181 ctcacgctgc cggaagtcga gccgccgtca cgcgtctggc aagccatcga gtccaggatc
     6241 cgccccggca ggcagggcag gccgggcttc tgggaaaacc tgtcgttctg gcgtttctcg
     6301 tccattgcta gcgggcttgt ggcgctggcg ttgctgatcc tggtagtggt gccgggcccg
     6361 gaagcgcccc cggctgatgg caccaggatg gtggtggtca tgaacgacct gaagaccagg
     6421 agcccggcga tgacagtgtc ctgggaaccc ggcatgccgg gaaaacgcgt catgcgcatt
     6481 cgcgtcatcg gccacgctga aatggcaccc ggcaccgcgt gggagctttg gatgctgcca
     6541 ggcaaggatc agaatccggt atcgctcgga ctgatcacca cccacgatgc ccagacggta
     6601 attgtccccg agccgctggc cacaaagctg gaccaggcgc agggcctggc gatgagcgtg
     6661 gaagccgctg gcggttcgcc caccggcttg cctaccggcc cggtgctcta cgccggccag
     6721 tgcgtaaaga catagacctt cgccacagcg ccagttcaaa cgcgttcaac gcaaaggacg
     6781 ccaaggtacg caaaggaaaa ccaaagtacg ggggtgaagt gcggaatcga gaatgcccgg
     6841 aaagtggatg ccgtcggacc gccggttggg tacgcgagta acgatcaggc cgacatctcc
     6901 actgagtaca aatttctggt tccctttttc ttgccctcct tcgcgtacct ttgcgtgctt
     6961 tgcgttgaac gccctcaagg tctagttctt aaggtgtcga ggtcagcgca gatcttttcc
     7021 gcggcatcga gaacgcgcgg cgtttgccgg gtgatgaggt cggcggaaac ggtgaaaatc
     7081 gcccccatcc gtaccgactt gagatagggg taacgctgcc agccttgtga gccggcgtct
     7141 tcggcttcgg agccgatgat gatggcatcc gggtccgcgg ccagaacctg ctccggcgac
     7201 acgactgcgg ccagttgcga cagatcgccg aacgcattgc gcgcaccgca gagccgcagc
     7261 acatcgctga tcagatgctc ggcgttgacg gtgatcagcg ggtgatgcca cgcctcgaac
     7321 aacacgctca ccgcagactt tccgcggtag cgctcgcgca accgctccgc gtgcgagtca
     7381 aaagccagcg ccgcagcctg cgcccgcctg ccgctcccca gcaacacgcc cagcatccgc
     7441 atatggcgcg ggatgtcctc gagacgccgc ggttccagta ccagcacggc gaacccgagc
     7501 ctctccagac gttccacatc cgcaacgcga ttgccgctgc cccacgccag taccaggtcc
     7561 gggcgcaatg ccaggatacg ctcgaagtcg agtcctgcag catcgccgac ttccgcgagg
     7621 gcccgcgccc cgggcgggaa atcgctgccc cgcacttttc cgaccagacg attgccagcg
     7681 ccgacggcga agaccagttc ggtgagatgc ggcgccagcg caatgacccg ctgcgccggc
     7741 caatcgcggc gcagcacaac gccgcgatcg tcgatgacct cgacggccgc cgcggcggtt
     7801 accgcgaaga actggaagac cagatttagg aaaaaaaaca ggcgccgcat tggcagcgca
     7861 agggctcagc gttccatgta gtggagcagg ttggaactgg tctggcgcag gcgctgcgac
     7921 agcagggtta caagcttgac caggaccttg gcgccgaggc tcggctcctc gaggatgatc
     7981 tgcaccatgc tgtcgcgcga cagcactgca aatgtagtgg gctccagccc catgcaggtg
     8041 gcaaagcgag gttcgccgtc gatcatcgac atctcgccga gcgttgcgcc ggacccgacc
     8101 gtcgtcatcg gctggagatg gccgtgcgaa tcggtcttca cgatttccac ccgtccctga
     8161 atgatgagca gcatgtaatc gtcgatgtcg ccttcgcgga tgatcatctg ccctgcttca
     8221 gcacggtaga tctgcatgaa accggtcagc ttctcgacgt cctcgcgggt gaagtccgca
     8281 aagaatttcg agtggccgat catgtcgaaa atctgttgcg cgagcgcggt cccctctccc
     8341 aggtgttcga gggtgttcag aagcatgttt tgcggctcct ggagttcggt atcagcaacg
     8401 ttcattggaa gtctggtcgg ggtctctcgc tccgtcattg ccgaagttgc cccccggaac
     8461 gcattcgacc cattatgcgg gagcggccgc aggcggtaaa gcccgatcca tgcggccgcg
     8521 ggtcgggttc aggaggtcgc cgtatcccgg taacgccgcg ccagctcggt acgcttgacg
     8581 caggcctcgc gggcaatcgc gacgtcggcg aggaaatggg gcagttcgtc cagtcgcagc
     8641 gcctgcggcc cgtccaccag cgctttggcc gggttgggat gaaaatccac cagcaccatg
     8701 ttcgcacccg ccagcacgcc ctgcgcggtg acgtgcatga cgtcgaggat gccgtcgggc
     8761 ccggccatgc ggctgcccac cgaatggctt gggtcgacgc ataccggcat gcgggtaagc
     8821 ctcttgaccg ccggcacatg ggagaaatcc acgaagttgc ggtgcgggtc gccgaggttg
     8881 gttttcatgc cacgcagacc gaacaacact ttgtgattgc cctcgctggc gaggtattcg
     8941 gcggcgttca gcgactcgtc gagcgttatg ccgaatccgc gcttgatcag caccggaaac
     9001 tccttctggc gcccgacgat cttcaacagc tcgaagttct gggtattgcg cgttcctatc
     9061 tgcagcatga ccccggtcgg gttgccggtg tcgcgcattg cctggcgaat ctcttccacg
     9121 tgcgattcgt gagtcacctc catcgcgatc accttgatgc cgtatttgcc ggcgagctcg
     9181 aatacgtatg gcaggcaggc cttgccgtgg ccctgaaagg catacggact ggtgcgcggc
     9241 ttgtaggcgc ccatgcgggt gcactgctgg ccgttgtcct tgagcgcacg catcatgatt
     9301 tcgacgtgct cgcggctgtc caccgcgcac agtccggcaa acacgtgcag cgtgtcctgg
     9361 ccgaagcgca gtccctggta gtcgaaatgc gtgggccgcg tgtcgccggg gtggcgtccg
     9421 aggacacggt attcctccga gaccctgatc acccgttcga cgcagggaag gctctgcatg
     9481 tcttcgatgg cgagcgcctt ggtatcgccg agcagataga cctcggtaac cgaccgctcg
     9541 gtgccctgga ttcggtgtat ccgcgtcttg atgttcggca ataacgcaag cgcctgcatc
     9601 aactgctcgt attccgggct ttgcggatcg gcgctgtcgc tgaggatcag aatcatgtgg
     9661 atacggtgat tgggcgattg ggtgattggg tgattggtta ttgagtgatt gggtaatttg
     9721 gtgaaacgcc gttgactcaa tcacttaatc actgaatcac tgaatcactg aattaccgaa
     9781 tcactgaatc actgaatcac cgcccttcgt cacgccgtgc ccccgacggt gagtccgtca
     9841 atgcgcagcg tcggttgccc cacacccacc ggcacgctct gaccttcctt gccgcaagtg
     9901 ccgatgccgg aatcgaggct catatcgttg ccgatcatcg tcacgcgcgt cagcgcgtcc
     9961 gggccgttgc cgatcagggt ggcgcccttg accgggtaag tcactttccc gttctcgatc
    10021 atgtaggcct cggcggcgga gaacacgaat ttgccgctga cgatgtccac ctgcccgccg
    10081 ccaaagttga ctgcgtacag gccgtgtttc accgaggcga tgatctcctg cggatccttg
    10141 tcgccgttga gcatgtaggt gttggtcatg cgaggcatgg tgatgtgcgc atacgattca
    10201 cgccggccgt tgccggtcgg ggacgcaccc atcagtctcg cgttgagcgc gtcctgcatg
    10261 tagcccatga ggatgccatc ctcgatcaga acgttgcgtt gcgtcggatt gccctcgtca
    10321 tccacgttga gcgacccgcg ccgccgggcg atagtgccgt catccaccac cgtgaccccg
    10381 gctgcggcga cgcgctcgcc gatacgcccg gaaaatgccg agctgccctt gcggttgaaa
    10441 tcgccttcga ggccgtgccc gatggcttcg tgcagcagca cgccgggcca gcccggcccg
    10501 agcaccacgg tcatggtgcc ggccggggcc ggccgcgccg acaagttggt cgccgcctgg
    10561 tgcaccgcct tggcggcgta ttccctgaga atgtcgtcgc tgaagtaagc gtaatcgaat
    10621 cgcccgccgc cgccggcact gccctgttcg cgccggccgt tctcctcgac gattacctgt
    10681 agcgacaggc gcgtgagcgg ccgcacgtcg gcggcaagaa cgccgtcgga gcgtgccacc
    10741 aggatcacct cgtactctcc ggccagcgtg gccatcacct gcaccacccg gctgtcgagc
    10801 gcgcgcgcat ggcgctccag ctgttccagc agcttcacct tgtcctggtc cgagaggctt
    10861 cccagcggat cctgcggcag atacaactcg cggccctggg ttcgtttcgc cacccgggtc
    10921 gtgcccacac ctccttggcg cgcgatggcg cgcgtggcgt aggccgctga ttcgagtgcc
    10981 ggcacgctga tgtcatcgga gtaggcgaac gcggtctttt cgccgctcac cgcgcgtacg
    11041 cccacgccct ggtcgatgtt gaagctgcca gacttgacca tgccttcttc caggctccag
    11101 ccctcggagc gcgaatactg gaagtacaag tcggcgtagt ccacgctgtg gctcatgatc
    11161 tgaccgaaca cgcgtgccag cccgccggcg tcaagcccat aaggagcaag cagcgtgctg
    11221 tcggcggtgg cgaacaacag ttcgtggttg tttgtgggat cgttgggcat ggagattcag
    11281 gggcaagggg cgagggacga gggtcgaggg gcgagggaag gaagactaga aatctggaaa
    11341 tgtcataggt tcgtgtttta tcttaattcg gggctcttac ccaagaattc aaatctgttt
    11401 tacgtagttc tccagtccct atctctcgcc ctcgcccctc gcccctcgac cctcgtccct
    11461 caaattctat gaatcgccct atgcgtaagc gccggcaggc tcttgcgcag gctggcctgg
    11521 taggccggat tgatccaggc gctgacgatg cccgaaccgc gcggcagccg gtcgagcacg
    11581 gttccccacg ggtccacgat catcgaatcg ccatgcgttt cgcgtccgct caggtgatag
    11641 cctccctggg ccggcgccag gacgtaggac aggttttcga tcgcacgggc ccggaccagc
    11701 gcttcccagt gggcgcgacc ggtcgtttcg gtgaacgcgg atggaaccac gatgatgtcc
    11761 acgtccttca tggcacgata cagctcggga aaacgcaggt cgtagcacac cgaaaggccg
    11821 atgcgcccga atggcgagtc caccaccttc acctgcttac ccggttcgat ggtgccctgt
    11881 tcagcgaact tctccttgcc caggtccagg ctgaacaaat ggatcttgtc gtagcgcgcc
    11941 accatttttc cggtgtcgtc gtagacctga caggaattgt agatcttgcc ctcaaccggc
    12001 gtttccatcg gcacggagca gcccaccagc cagatcccga gtctggccgc ggtttcggcg
    12061 agaaacgcct ggattggtcc gtcaccgtct ttctcgcgga ccttgacctt gtccgtgggg
    12121 cgcattccca tgatggcgaa atactccggc aacgccacca gtttcgcgcc ttccgccacc
    12181 gccatttcga tcagccggcc agcttcattc aggtttgcgg acacgttcgg cccggaggcc
    12241 atctgcaccc cggcaatgcg aaagccgtgc ggcgggttct tcgacgcgcc agcgggagaa
    12301 tgttcgcgtg cgccgcgccc tttggcgcgg ccgaatcccg atctgttgat ggttgccata
    12361 tctgctctgg accgttgcaa cgagcgcgaa ttgtaacctg ctacgagtct tcccctagcg
    12421 ccgcgacggg ggactctcga cgggcttgtt gatgcgcgcc actaccgggt cggcccaggt
    12481 gcccgttacc gaatactcga acgacgcgat cttgctcaac gggtccttga gcgctttctg
    12541 ggcaaggtag gccgccacgc ccaccgccgg gttcacgatc gcgccggcca ccgcaatgct
    12601 ttccgacagc gaaggcgtga cccgcacgat cagattctgg gtctcggccg cgaggtccac
    12661 ccggccgcgc atgcccacgc gcgccgccga gccttccatc atgaagtctt cggtatgcac
    12721 cacgccgttg gcaatgctca gattggcgct gatgcggtcg aacgtgaagc ccttgctgaa
    12781 aacgtcctgg aaatccagcg acgcccggcg cggcaacgcc tgcaggctga ggatgccaag
    12841 cagctttgcc acacccggct cgatctggcg aaatcggcca ttcttggctt ccagtttgag
    12901 ctgaccggaa agcgacggga tgtccacccg atacgggcta cccgcccaag cgaccggccc
    12961 ttccagcagt gctgagccgc ccttcacgcc ctcatgccag ccgaccctcg aaaagaattt
    13021 gccgatgtcg ttcacttcca gccgcacttc cacgtccgtg cgcgatacca cgacaaccgc
    13081 ccatcgcccg ttgatgctca gcttgctctc cgggttggtg atctccagac gctgcaggcg
    13141 ccagcccgac ggctcaggat cggcgagaac ggtaagctgt ccgagatcct tgccctcaaa
    13201 cgtgaaactc tcggcaacca ggtcgaccga cggccggcgc tcactggaaa agctgtccgc
    13261 tgcgccggtc tcaagagtgg tggtcagcgg cggcagcgtg agccgggtca ggcgcgcaga
    13321 caggcgcccc tcgcctgccg atgcccatga caactgaccg gcgatctcgc gccccgatag
    13381 cgccgcctgc catgccgagt cctgccttgt cgcattgacc ctgaggtcgt gaaatcgcct
    13441 gcgatgcgca tcgaggatgc caatctgcag atcggcgccg gcaagatcga cagacgggct
    13501 gccagaacca cctgcgaaaa tggggcgcca gctatccacg tccaccacat ccaggctacc
    13561 gttcacccac aagccatcgg ccgccggcag cgaagcagtt gcgccgaggc ttaccatgcc
    13621 gcgctttatg ccatcaggat ccgccgaatc cagcagcacc cgcgccgagg ccaccttgcc
    13681 caggtcgatc gcgagcaggc gctggcgccc ggggcgctcg cgcaactcca ggtgcaaggg
    13741 caggctggtg cccgcggcct ttgcaaacgg ggcgggcaat gtcgagccga ttcccaccag
    13801 actcgaatcg atcctgaggc tggccatctt gtcgcgtacg ccgatgttgc cgcgccattc
    13861 ggtctgccct tccacgaact gcagcgcggg atggttcgat acgctgcgca agcgggtcgc
    13921 gtccagcgtg cccgcgacat tgagcaccat gccgccgtcc ttttggttgg aggcttcgaa
    13981 ggaaatagga ttgccggcga gccgggcacg gccatccttg acgctcaccg tttttttggt
    14041 aaaggccata cgcgcgccaa atccttccag cggcggcagg cgcgggtcca cggtaatctg
    14101 attgtcgcgc aacaccagga ccccggagat cacggagtcg gcgatctgcc gcaacggcag
    14161 gtcgagctcg acatcgagcc gggcattacc gctcgccttc atgtcctcgg tgaaccggtt
    14221 gatatggcgc gacaccgggc tggattcgac gaagcgcaga aactccgcgg ttggcccttc
    14281 ggccacccct ttcaccagca gatgctcatc gtgcttgccg agttcgggga tggaggccag
    14341 cacgtcggat agccgcagcc ccaggatcga acctgcctga gggcgtactt ccatgcgccg
    14401 gccgcgaaat accacgtcgg cgacggcgtt cgtcagcggt ggccagccgt cggcatagtc
    14461 gagcgtgacg cccgtcaccc gtgtcttcac ctcgaacaat ccggtcttat cgttctcgaa
    14521 cggaaaatca ttgagcggac cctgcaggcg gaaacgcacg tcgcgcgact cgcctgccag
    14581 caacgcccgc ctgagccacg cctgtgtcac cggggcgaac accgggatgt agcgccacag
    14641 ttggcgcgca tcgacgcgca ccatcgagcc cgccagatcg gcgctgccgc ggtctccttc
    14701 gggatcggtg cggtaccatc cgtacacatt gccggccagg tgcgcgttgg taaacgcaac
    14761 gttcttgagg gtaaccagtg tgtgcccgtc ctggaacttc cagcctgcct cagcggtaag
    14821 aacgtccagc gtgatttctt cgctgaacac gcgcggcaat tcgacgtgag catcgcgggc
    14881 gctgagcgac accgtgccgc caccctgatc ggcgtcaaac tggccattga tgccgcgaaa
    14941 tccggggaat accccgtccg gcttcaacgt gaggccggag aaccgcgcgt gggcacggta
    15001 gggccgctcg gcgttccact cgccctccca ggaaaacagc gcttcgtcga ccatgccact
    15061 cggtgaggag cgccccagcc ggcctcgcag cgatgcgtcg aggggaaggt attcggccag
    15121 ttccaccacc ggcgcaagat cgagcttttc caggcgcagt tccgagtggc cgccaccatc
    15181 gcccgtccca gtgcgggtaa aggacaattg catgggcgcc agcgtcaatc cgtcggcggt
    15241 ggtaaaaccg agcgcctgtg cgctgatctg cgtgcgcccc ccgcgactgt tccaggcaag
    15301 ccgaccctgc agattggcaa gcgcaagctc gggaagatcg ggcgcgagcc gcgcctgcac
    15361 gttgaccaga cgcaagtcgg cggtcgccgc ggctacgccg gggccctcga ggtccatcca
    15421 gacgtgcagc gaaccgagcc cgcgcgcaac atcgaagggc actgcaatcc aggtcttagc
    15481 ctgcgccaga tcggcgtaac cgaactccag gtacaggcgg ccgcgccagg cctgcagatc
    15541 gtgcgcgctg cggccccaca gctcaccgcg agccacgatc tgcgaggcga cttcggcagg
    15601 aggcgaggcg ttcagtccaa agcgatggct ttcgccgtcg ctgtcgagac gaatgttcac
    15661 gctggtgagc ctcagatccg gcgcggcgcg ggtttcgtcg cgccagacga tctgcgcact
    15721 gcgcaccagc acctgacgct gcgtcagcag ccagtccgca aatccgccgc cccgcccctg
    15781 gctgcgctcg accgcaatgc cggcgagcca cagctcgccg gcggcgtcgc gtctgacttc
    15841 cagttccggt tcgacgatct cgagcgactc gaaccgcacc tcggcatcga gcaacgacaa
    15901 ccacgacagc accgcttcga cccggcccag actcagcgct tgctggtccc gcgtatcgaa
    15961 gacgcgcaca tcctgaaaac tcagctcggg ctggtagccc tgccaactgc cggtgatggc
    16021 gccgatcgag acccgctgtc ccgccgcccg gctgaccgcc tgcgcaattg cctcgcggta
    16081 atcgttgatg ttcggcagca ccacatatcg cagtccgatc accagcgcga cgaacacgat
    16141 gccggcggcc agcaccgcgt aagtcgcgag gcgataagtc cagagcgaac catgcttgag
    16201 aatgaagagg ctatgtttga gaaccacggc gcggcgttgt cagggcaaag gaaaaacggt
    16261 cttcagttaa gatacacggg cggcaataag attccccgga tgctctggct ttgcccgcat
    16321 gatgcctgcc aaacttgccg gcgcaccgtt gacgcgcgag acgccgatgc gttgacatcg
    16381 gccgggcgcc aaccccgatt cgggtatcct gctctcaagg atttccggat tctagactac
    16441 ccgggcatgt cttcccccgc actttcgaac ggcacgcaat ccgaaagcat cgcgcgtgca
    16501 gcgcggctgt cgcgctacgc gcgtcacctg ctgtccgcca accccgcgct cgcggccgaa
    16561 gcggctgtgg atctgccgtt cgcgccagac gagatgcgcg cgcgcctttc cggcgccgac
    16621 ttcggcgacg aggacgcgct caagaccggc ctgcgccggc tgcgtcaggc ggtcatgctg
    16681 cgtctcatca cccgcgactt gggcgggcgt gccgatcttg ccgaggtagt cgcgaccgcc
    16741 acggcgctag cggaggaatc cctgcgcacc gcggccgcac gcctcgcgca atggctgaca
    16801 cgcgatttcg gccggcccgt tggcggtgca tcgggagacg tacaggaatt gcacgtgatc
    16861 ggcatgggta aactcggcgg cgccgaactc aacgtgtcct cggacatcga tctcattttt
    16921 gcctatcccg aagacggaga aaccgacggc gcgcgccgga tctccaacaa cgaatacttc
    16981 actcgtctgg cgcgcaaact aatcgccgcg atcggcgagg tcaccgcgga cggcttcgtg
    17041 ttccgggtcg acacgcggct caggccctac ggcgacagcg ggcccctggt ggcgagcttc
    17101 gacatgctgg aagactacct cgccacccag ggacgcgaat gggaacgcta cgcctgggta
    17161 aaagggcgga ccatcagcgg cgaccgcggc gcgcagctcg aggccatcgt cgccccgttc
    17221 gtgtaccggc gccacctcga cttcagcgcg ctggccagcc tgcgcgagct gcaggcgcag
    17281 atccgccagg aagtgcaacg gcgcgagatc gccgacaaca tcaagatcgg ccgcggcggc
    17341 atccgcgaga tcgagttcct ggtacaggta tttcagatca ttcgcggcgg gcgcgagccg
    17401 cagttgcgcc tgcggccgac gctgaaagcg ctggccctgc ttggagagaa acagttactg
    17461 ccggcggcgg cggtcgggga actgcaagcg gcgtacctgt ttctgcgcaa cctcgaacac
    17521 cgcttgcagt acctggatga ccagcagacg cagatgctgc ccgcgggcgc ggaagaccgg
    17581 cagctggtcg cagaaatgat gggctatccc gcctatgccg acttcgaccg cgacctggcg
    17641 ggacatcgag gcgtggtgtc ccggcatttt gaagcgatct tcgccggaca acgcgaagaa
    17701 gcgacggcag atccctgcgc cgacctctgg ctgcaacagt tacccgagaa agatgcgctg
    17761 gcccggattg ccgcgttagg ctacgccgac ccggcgcgca tactcctgct gatttcggac
    17821 atccggtcca gcacccgcta tcgccgcatg gcggcctccg gccaggcgcg tctggaccag
    17881 ttgatgccga aattgctgaa gctcgccacc accctccccg gcccggacgc gactttcgag
    17941 cgggtgctgc aggtggtgga aagcattggt cggcgtgagt cctacctggc gcttctgctc
    18001 gagtatccgc tggcgctggc gcgtctcgcc cgtctggctt ccgcaagccc ctgggcggcc
    18061 gggtatatgg cacagcaccc gatcctgctc gacgaactga tcacgccgca aagtggacag
    18121 gcgccggact ggccccagtt gcgcagcgcg ctgcgcgcca gcctcgacga acacgccggc
    18181 aataccgagc ggcagatgga tatcctgcgc cacttcaagc agacgcagac gctgcgcctg
    18241 cttgcactcg accttggcgg aaacctgccg ctggaaacgc tgtccgacca cctcagcgat
    18301 ctcgcctgcg tggtgctgga ggaagtgttg cggctggcct gggacggcct gagggtacgc
    18361 cactgcgacc aaccgcgctt tgccatcgtc ggttacggca agcttggtgg caaggaattg
    18421 ggatacgcgt cggacctcga catcatcttt ctctatgacg acgcgcatga gagcgcgccg
    18481 gaaatctacg ccaggctcgc gcagcgcatc aacaccctgc tcaccagcac cacctccgcc
    18541 ggcgtgctct acgaaaccga cctgcggctg cggcccaacg gcgtggccgg cttgctggtg
    18601 agccacatcg acgcattccg cgactaccaa ttcaaggacg cgtggatctg ggaacaccag
    18661 gcacttacgc gcgcgcgcgc agtcgccgga gacgcgcaaa tcggcgcccg cttcgaggcc
    18721 aacaggatcg atctgctgcg caggcggcgc gacctgactt tgctcagcca ggaaatccgc
    18781 gcgatgcgcg acaagatgcg ccagagccat cccaacgaca cccggctgtt cgatctgaaa
    18841 cacgatcgcg gcggcatcgt cgacgtcgag ttcatggtcc agtacctggt gctcggcgaa
    18901 gcgcaccacc atgccgagct gaccggcaac atcggcaatc tggctttgct caagctcgct
    18961 gcccggctcg ggctgatacc ggaagacctg gcagcggccg cgcatgcggc ctatcgccag
    19021 ttccggcgcc tgcagcacca gttgcgcctg cagggcgagc gctacgcgcg cgtcgacccg
    19081 gcgaccgtgg ccgaacatgc gcaagccgtg gtcaagctat gggataaggt cttctcagaa
    19141 caaccggcct aaaaaaaagc gctcaccacg gaggacgcgg aggacacgga ggaaaaccag
    19201 aaacgaggaa aaacatgtgg gggttaaagg cagacgcggt gctgaagtga cctccgatgg
    19261 caagttttag ctctcgaaga ttccttttct gttctcgtct ttcccccgtg tcctccgcgt
    19321 cctccgtggt gagtgtttgt ctgttacacg gacccgttgc ccggacaccc acaggaccat
    19381 ctatcgaatc atttggatta gaaaagccta gccggagtag agcttgccgg taagacggta
    19441 aaccgtggtc tcgcccttgc ctttgatcga gatcgtgcgc ggctcctcga aggaatagcg
    19501 gtgcccgagc cggcgataag tggtcttgtc gaccaggatt atgccgccgg ccgcctcggc
    19561 ggtgagccgg ctggacaggt tgacagtatc gccccacagg tcgtagatga agcgcgtggc
    19621 gccgatcacg cccgcgatcg ctgggccggt cgcgatcccg atgtgaagct gc
//
LOCUS       DASQOP010000117         4436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1857989, whole genome shotgun sequence.
ACCESSION   DASQOP010000117 DASQOP010000000
VERSION     DASQOP010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1857989"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1627
                     /locus_tag="VE030_04610"
     CDS             <1..1627
                     /locus_tag="VE030_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22735.1"
                     /translation="EPLKAVATLQALPEEHITQKCLSMGPESDAPGSDAPFLTNARLT
                     LNARGDAVEISTDSPVSSPTLALVLRVQCPGAFFYARHFTMLIPPAPKAAQASPQRPP
                     RGFTLRVGAGESVESLAVSVFPRSRNLQRLLVDEVVKLNPQVFPSRTLRPVAPGTVLF
                     FPELRGLPERAGRPAASGRAAMPPARKVPAATQPDTVIATPVKRSVRRRAVAAKPGEP
                     VTLRRALELGERPGPEECRALMPLCGVEQALGLVPPALEEKARGIESGVQELRLKQES
                     IDSQLARLEQSLAALRKIVETAPVQPAPPAPAPKPEIRTVVKTEPMPWYYWAWFAAIV
                     AACAVGGFAFGRRQAYTGTLGEADERLDQMLASAATAIRELDAEPTLTPKRVVARPEP
                     PPPKAKPEPAPARPARTDTKPLAQAGPQKANVDLAPVTQAIKPGTNVDVKLESAPAAQ
                     SVGLSSKVLFEMDQALDNTRSMFTDVDRFIALGRTQNAISLLQFQVHKDPKDRDSWIK
                     LMAIYRQEKMDAEFAAAVREFKRHFPGDDASTG"
     gene            complement(1643..3031)
                     /gene="dnaB"
                     /locus_tag="VE030_04615"
     CDS             complement(1643..3031)
                     /gene="dnaB"
                     /locus_tag="VE030_04615"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006163610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HZF22736.1"
                     /translation="MAQFATQRIADTQVELIKLPPHSVEAEQSVLGGLLLDNSAWDKI
                     ADMISESDFYRADHRLIYRSISRLIEHSRPADVITVAESLESTKELETVGGIAYVGAL
                     AQNTPTAANIRRYAEIVRERAVMRKLAEVGTEIADAAYNPMGKEAGQLLDEAESKVFA
                     ISEEGARGRQGFVEMPPLLTQVVERIDLLYNRDNPSDVTGVATGFADLDRMTSGLQPG
                     DLVIIAGRPSMGKTSLALNIAEHVALEAGLPVGIFSMEMSGTQLVMRMLGSVGRLDQH
                     KVRTGRLADEDWRRLTDAVGKLNEAPIHIDETAALNALELRARARRLHRQYGRLGLIV
                     VDYLQLMSASAQGENRATEISEISRSLKALAKELNVPVVALSQLNRSLEQRPNKRPVM
                     SDLRESGAIEQDADVILFIYRDEVYNPDSPEKGKSEVIIGKQRNGPIGTVTLVFQGEY
                     TRFANFADPGRF"
     gene            complement(3183..3638)
                     /gene="rplI"
                     /locus_tag="VE030_04620"
     CDS             complement(3183..3638)
                     /gene="rplI"
                     /locus_tag="VE030_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010809515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HZF22737.1"
                     /translation="MQVILMEKVHNLGQLGDIVKVKDGFARNFLIPQGKAKRATDRAI
                     TEFQSRRAELELQQGKALAESQARAARLEGLMIQITQNAGVDGRLFGSVTNYDIAETL
                     KAQGHEVTKAMVRMPQGPLKQIGDYPITLDLHADVTVTITVSVLGETVS"
     gene            complement(3651..3932)
                     /gene="rpsR"
                     /locus_tag="VE030_04625"
     CDS             complement(3651..3932)
                     /gene="rpsR"
                     /locus_tag="VE030_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HZF22738.1"
                     /translation="MPRFGGSKGKGKDKGKERSNRPLFRRRKFCRFTAEGIKEVDYKD
                     VEILKDFINENGRIIPARITGTKTHFQRQLSVAIKRARFLALMPYTDLH"
     gene            complement(3945..4241)
                     /gene="priB"
                     /locus_tag="VE030_04630"
     CDS             complement(3945..4241)
                     /gene="priB"
                     /locus_tag="VE030_04630"
                     /inference="COORDINATES: protein motif:HMM:TIGR04418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HZF22739.1"
                     /translation="MSRNQVVLTGKLIERNELRHTPAGLPALNFRIGHISEQVEAGRS
                     RSVNLEIEGVALGEVAQKLSKAVQEAEYRFEGFLAMRSKLSRQPVLHVSRFELN"
     gene            complement(4246..>4436)
                     /locus_tag="VE030_04635"
     CDS             complement(4246..>4436)
                     /locus_tag="VE030_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HZF22740.1"
                     /translation="TLTELEHAYKFNDAVVRHLIVNMTRAVTEPSPMMKEEKSRTVVG
                     ESVKPEAAAEKPAPAAAQ"
BASE COUNT          765 a         1422 c         1378 g          871 t
ORIGIN      
        1 tgagccgctc aaagcggtgg ccaccctgca ggcgctcccg gaagaacaca tcacccagaa
       61 atgtctgtcc atgggcccgg agagcgatgc accgggctcc gatgcccctt tcctgaccaa
      121 tgcccgcctc acgctgaacg ccaggggcga cgcagttgaa atttccaccg actcccccgt
      181 ttcctccccc accctggcac tggtccttcg ggtccagtgt ccaggggcct ttttttacgc
      241 gcgccatttc acgatgttga tcccgcctgc cccgaaggca gcgcaggcat cgccgcaacg
      301 cccgccgcgg gggttcacgc tgagggtcgg tgctggtgaa tcggtcgaaa gcctcgccgt
      361 ctcggttttt ccgcgcagcc gcaatctgca acgcctgctg gtcgatgaag tcgtcaagct
      421 gaatccacag gttttcccga gcaggacgct taggccggtc gcgccgggga cggtgctatt
      481 tttccccgag ctccgcggtc tgcccgaacg cgccggcaga cctgcggcga gcggccgcgc
      541 cgcgatgccg ccggcgcgga aagtacccgc ggcaacgcaa cctgataccg taatcgcgac
      601 cccggtcaaa cggtccgtca gacggcgggc cgtcgccgcc aagccgggag aaccggtcac
      661 gctgcgccgg gctctcgagc ttggcgagag gccggggccg gaggaatgcc gggccctgat
      721 gccgttgtgc ggcgtcgaac aggcgctggg cttggtcccg cccgcactcg aggagaaagc
      781 tcgcggcatc gagtccggag tgcaggaact gcgcttgaaa caggagagca tagacagcca
      841 actcgcccgg ctggaacagt cgttggcggc gctgcggaaa atcgtggaga cggccccagt
      901 gcagccagcg ccgccggcac cggcgccgaa gccggaaatc cgcaccgtgg tcaagaccga
      961 accgatgccg tggtattact gggcgtggtt tgccgcaata gtcgcggcct gcgctgtcgg
     1021 cggctttgcc ttcggtcgcc ggcaggcgta taccgggacg ctgggcgagg ccgacgaacg
     1081 cctggatcag atgctggcgt ccgccgcgac cgcaatacgc gagctggatg ccgagccgac
     1141 gctgacgccc aaacgcgttg tggcgcgccc cgagccgccg ccaccgaaag caaaaccgga
     1201 accggcgccg gcacgcccgg cgcgcaccga taccaagccg ctggcgcagg ccgggcccca
     1261 gaaggcaaac gttgatttgg cgccggtcac ccaggcgatc aagcccggca ccaacgtgga
     1321 cgtgaaactg gaaagcgccc cggctgcgca gtcggttggg ctatcgagca aagtcctgtt
     1381 cgaaatggac caggccctgg acaacacccg ctcgatgttc accgacgtgg accggttcat
     1441 cgcgctggga cgcacgcaga acgccatcag cctgctgcaa ttccaggtcc acaaggatcc
     1501 gaaagaccgc gattcgtgga tcaagctgat ggcgatttac cgccaggaaa aaatggatgc
     1561 ggaattcgcc gccgcggtgc gcgaattcaa gaggcacttc ccgggcgacg atgcgtcgac
     1621 aggctagtgc gggctaacga tttcagaagc gcccgggatc ggcgaaattg gcgaagcggg
     1681 tgtactcgcc ctggaacacc agggtcacgg tgccgatcgg accgttacgc tgcttgccga
     1741 tgatgacttc cgacttgccc ttctccggag agtcggggtt gtaaacttcg tcgcggtaga
     1801 tgaacaggat cacgtccgcg tcctgttcta tggctcccga ttcgcgcaga tcagacatca
     1861 ccggccgctt gttgggccgc tgctcgagac tgcgattgag ctgcgacagc gccactaccg
     1921 gcacgttcag ctccttggcc agcgccttga gcgagcgcga gatctccgaa atttcggtgg
     1981 cgcggttctc gccctgcgcg ctcgccgaca tcaattgcag gtagtccacc acgatcaggc
     2041 cgagcctgcc gtactggcgg tgcaagcgac gcgcacgcgc gcgcagttcc agcgcgttga
     2101 gcgcggcggt ttcgtcgatg tgtataggcg cttcgttgag cttgccaacc gcatcggtga
     2161 gccggcgcca gtcctcgtcc gccagccgtc cagtacgcac tttgtgctgg tcgagccggc
     2221 cgacgctccc aagcatgcgc atcaccagct gcgtgcccga catttccatg gaaaatatac
     2281 ccaccggcag tccggcctcc agcgccacgt gctcggcgat attcaaggcc agtgaggtct
     2341 tgcccatcga cggccttccg gcaatgatca ccaaatcgcc cggctgcaac ccggatgtca
     2401 ttcggtcgag atcggcgaag ccggtcgcta cgccggtgac gtccgagggg ttgtcgcggt
     2461 tgtacaggag atcgatgcgt tcgaccactt gcgtgagcag cggcggcatt tccacgaatc
     2521 cctgtcggcc gcgcgcgccc tcctccgata tcgcgaatac tttcgactcg gcctcgtcga
     2581 gaagctgtcc ggcctccttg cccatcgggt tgtacgcggc gtcggcgatc tcggtgccca
     2641 cctcggcaag cttgcgcatc accgcgcgtt cccgcacgat ctccgcgtag cggcggatgt
     2701 tggcggcggt gggagtgttc tgcgccagcg cacccacata ggcgatgccg ccgacggttt
     2761 ccagttcctt ggtgctctcc aggctttccg caacggtgat gacgtccgcg ggccgggagt
     2821 gttcgatcag tctggagatg ctgcgataga tgagccggtg gtcggcgcgg tagaaatcgg
     2881 actcgctgat catgtcggcg attttgtccc acgccgagtt gtcgagcagc aggccgccca
     2941 ggacggattg ctcggcctcg accgaatgtg gcggcagctt gatcagctcg acctgggtgt
     3001 ccgctattct ttgtgtcgcg aactgcgcca tttgtgccgg agatgttttg ggtgggtgaa
     3061 caaatgtggc gtcaaacagg aaccgtcgcc gactattatt cgtaacgcga tgaggcgcgc
     3121 aagcggaaaa ataaaaaggg ctgttttcac agcccttttt tttgcgccga agccctgcgg
     3181 attcagctga cggtctctcc cagcaccgat acggtgatgg tcaccgtgac atcggcatga
     3241 agatcgagcg taatcgggta gtcgccgatc tgcttcagcg gcccttgcgg catccgcacc
     3301 atcgccttgg tgacctcgtg gccctgcgcc ttcagcgtct cggcaatgtc gtagttggtc
     3361 accgagccga acaggcgccc gtccacgccg gcgttctggg tgatctggat catcagccct
     3421 tcgagcctgg ccgcgcgcgc ctgcgactcc gcgagcgcct tgccctgctg caactcgagt
     3481 tcggcgcggc ggctttggaa ctcggtgatc gcacggtccg tggcgcgctt ggccttgcct
     3541 tgcggaatca ggaagttgcg cgcaaagccg tctttcactt tgacgatgtc gccaagctgg
     3601 ccgaggttgt gcactttttc catcagaatg acttgcatgg ctacggttcc tcagtgcagg
     3661 tcggtgtacg gcatcagcgc aaggaaacgc gcgcgcttga tggcaaccga caactgacgc
     3721 tggaagtgcg tcttggtgcc ggtaatacgg gccggaatga tcctgccgtt ctcgttgatg
     3781 aaatccttca ggatctcgac gtccttgtaa tccacttcct tgatgccttc ggcagtgaag
     3841 cggcagaact tgcggcggcg gaacaacggg cgattgctgc gttccttgcc cttgtccttg
     3901 cctttacctt tgctacctcc aaatcgtggc atggctctct atcctcaatt caattcgaat
     3961 ctgctcacgt gcaacaccgg ttgtcgggac agcttgcttc tcatcgccag gaagccttcg
     4021 aacctgtatt ccgcttcctg caccgctttg ctcaatttct gcgccacttc gccgagcgcc
     4081 acgccttcga tctcgaggtt gacgctcctg gacctgccgg cttcgacctg ttccgaaata
     4141 tggccgatgc ggaaattcag cgccggcagg ccggcgggtg tatgacgcag ttcgttgcgt
     4201 tcgatcagct tgcctgtcag gacaacctgg ttgcgactca ctgcgtcact gggcagctgc
     4261 cggcgccggt ttttccgccg cggcttccgg cttgacgctc tcgcccacca cggtacgcga
     4321 cttctcttcc ttcatcatcg gtgaaggctc ggtcacggcg cgggtcatgt tcacgatcag
     4381 gtggcgcacc accgcgtcgt tgaacttgta ggcgtgctcg agttcggtca gggttt
//
LOCUS       DASQOP010000118         7790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_452745, whole genome shotgun sequence.
ACCESSION   DASQOP010000118 DASQOP010000000
VERSION     DASQOP010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7790
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452745"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VE030_04640"
     CDS             <1..271
                     /locus_tag="VE030_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase N C-terminal domain-containing
                     protein"
                     /protein_id="HZF22741.1"
                     /translation="VIGSFCQGNHVRFHTADGSGYAFAADQVIALDPLNPQVAARLAR
                     SFDRWRKFDTIHQKHARAALERIRETAGLSMDTTEVVTKALATSP"
     gene            complement(348..1487)
                     /locus_tag="VE030_04645"
     CDS             complement(348..1487)
                     /locus_tag="VE030_04645"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZF22742.1"
                     /translation="MRNAIAGFLASSVVVLATAVPSQAADPIVFGLVDEVTGPQAEAG
                     MLTAYGARLAQEEINASGGILGRPVELQIEDNQSTNPGTVLAYSKLVGKKVVAVIGPL
                     RSTQVQAVSPTIAKARIPALIGGSDPSLTRVNNPWIFRVRPNDLYSSKVMAEFGVKKL
                     NGAKWAIIHSTDTFGTGGKNALVDALKAYGIEPVLVQGYTNNQQDFTPIVLAVKKSGA
                     NVIGSYMTNAPDVGIFARQLRQLGVTIPWVGSTSIVTETAIRLAEEALWGTYGVSDFV
                     VDANQESKMFAANFRKKHGFEPDLFSAWSYGGMYLLKHAIEKAGTTDADAVREAMLGI
                     RGLKGVEGTYNFNSNGDGAHGYNIVKNEKGKVVFIEHIEFPPTSP"
     gene            complement(1613..2554)
                     /locus_tag="VE030_04650"
     CDS             complement(1613..2554)
                     /locus_tag="VE030_04650"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZF22743.1"
                     /translation="MAALPSLRQLHYLVEVADKRNFRAAAEASFVTQSTLSAGIKELE
                     TLLGVELVERDPRGVRLTPAGEEVTRRARTLLAAAQDLVETARGAGAPLSGLFRLGVI
                     PTIAPFLLSGVLAALRQAYPELKLYLREDQTERLLEKLRAGELDATLIALPFDTGELT
                     VRPLFRDEFWFVARQDDPHAKSKEVLVRDLEPGSVVLLEEGHCLRDHAIAACGARGAK
                     AKAMVEATSLHTLIQMVEGGLGVTLLPELTLKAGILNGTQLIARPFATQVPARTVALV
                     ARPTGARAADVDLLAVFFEGHARKAARLRVPSRRKNR"
     gene            2676..3215
                     /locus_tag="VE030_04655"
     CDS             2676..3215
                     /locus_tag="VE030_04655"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZF22744.1"
                     /translation="MLTIGDRLPAFDLAAVVSTEQDKAFTRITGASNPGKWKVLFFWP
                     KDFTFVCPTEIAAFGKLNADFNDRDALIYGVSIDSEFVHLAWRNNHADLTDLPFPMLA
                     DIKRELSQALGVLDKKEGVALRATFIVDPEGMIRFVSVNDMNVGRNPEEVLRVLDALQ
                     TDELCPCNWKKGDEFLKAA"
     gene            3460..4011
                     /locus_tag="VE030_04660"
     CDS             3460..4011
                     /locus_tag="VE030_04660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZF22745.1"
                     /translation="MSIQTLKDRLPEYAKDLKLNLGSLAAETVLNDTLRAGTFVAAAF
                     ASRNAEVIAAVNAEFAGKLAPADLTAAKAAAAIMGMNNVYYRFLHLSENAEYQRLPAK
                     LRMNVIAAPGTARENFELWSLAVSAINGCGLCITAHDKVVRAAGITAEQVQAAVRIAA
                     VVHAVAVTLESEAAANVELAAAA"
     gene            complement(4108..4554)
                     /locus_tag="VE030_04665"
     CDS             complement(4108..4554)
                     /locus_tag="VE030_04665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HZF22746.1"
                     /translation="MPVMEWDDNLVLDQGVMDDTHREFIGLLNRLADAPDSETLAVLD
                     EFIAHTEAHFSQEQRWMEQMSFPPAECHVREHDGVLEIAREVHRRAAAGETGFGRVLA
                     KAVAEWFANHAASMDNVLALYMKEVGFEPDTHLSPALSPQRAEREG"
     gene            4727..5611
                     /locus_tag="VE030_04670"
     CDS             4727..5611
                     /locus_tag="VE030_04670"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HZF22747.1"
                     /translation="MPELPDIVVYIENLESRIQGRILERIRLLNPFVLRTAIPPISSA
                     EGRKVLGLRRLGKRIVIGLEGDLFLVLHLMVAGRLRWLEGEARLPGKIALAAVEFDGG
                     TLVFTEAGTKRRASIHLVQGEAAMNALHAGGMEVLESGQAAFSQRLKAENHTLKRALT
                     DPHIFSGIGNAYSDEILHRARLSPIALTGTLGDEEVLRLFEATRSVLGEWTDRLRKET
                     GGGFPEKITAFREGMAVHGRYGKPCPVCGSPVQRIVYADNETNYCARCQTGGRILADR
                     ALSRLLKKSWPRSLEEIE"
     gene            complement(5622..6386)
                     /locus_tag="VE030_04675"
     CDS             complement(5622..6386)
                     /locus_tag="VE030_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009935950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HZF22748.1"
                     /translation="MSVFSPSEVEYFESNGFVIARNLAAHELCERMKSLAQRHLAQAV
                     QPVEYEAQTKYPGSPVSLDAPGGKTVRRLLQAYARDPVFGDWATSRPIGGRLRLLLGP
                     QVELSQAHHNCVMTKNPAFSSATSWHQDIRYWSFEKPELISAWLALGQEHEENGCLLL
                     IPGSHRIEFRREQFDDALFLREDVEENRRILDGRMTAELDEGDVLFFHCRLLHAAGRN
                     RTGLTKFAAVFTYHSTGNRPLPGTRSASLPEISLKA"
     gene            complement(6534..7589)
                     /locus_tag="VE030_04680"
     CDS             complement(6534..7589)
                     /locus_tag="VE030_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HZF22749.1"
                     /translation="MTYIAKPRLHHPQLALNKLGYTRRDYEGAISTLCAGCGHDSISA
                     AVVQACFELDLPPHRIAKLSGIGCSSKTPTYFLGSSHGFNSVHGRMPSVLTGANLANR
                     DLIYLGVSGDGDSASIGLGQFAHSLRRGVNMVYIVENNGVYGLTKGQFSATSDRGSKS
                     KKGALNTDAAIDMVMLAMELGATYVARGFSGDKTQLVPLIKGAIVHRGAAFIDCISPC
                     VAFNNHVGSTKGFDYVREHNEAVNRLDVMMPRESITADYAPGEVEEVTQHDGSVLRLR
                     KLHAEYDVHDRIAAMNYLQERKAAGEIVTGVLHVDPDPEDLHAHLETVETPFNRLAER
                     DLCPGVEALEKFNAAHR"
BASE COUNT         1485 a         2474 c         2339 g         1492 t
ORIGIN      
        1 ggtgatcggc agcttctgcc agggcaacca cgtgcgcttc cacaccgcag acggttccgg
       61 ctatgcgttt gccgccgatc aggtgatcgc gctcgatccg ctcaatccac aggtcgccgc
      121 gcggctggcc cgctcgttcg accgctggcg caaattcgac acgatccacc agaaacacgc
      181 cagggcagca ttggagcgca tccgcgaaac cgccggcctg tcgatggata ccaccgaagt
      241 cgtcacgaaa gcactggcca cctcaccctg acgcgcggac acgttgctga caaggtcagc
      301 cgaatcttaa ttcagaactc tgttccctct ccgctccggg gagagggcta gggagaggtg
      361 ggcgggaact cgatgtgctc gatgaaaacc actttgcctt tttcgttctt gacgatgttg
      421 tatccgtgcg cgccgtcgcc attggaattg aagttgtaag taccttccac tcccttgagc
      481 ccgcgaatgc ccagcatcgc ctcgcgcacg gcgtcggcgt ccgtggtccc cgctttctcg
      541 atcgcatgct tgagcaggta catgcctcca tacgaccagg ccgaaaacag gtccggctcg
      601 aagccgtgtt tcttgcggaa attggcggcg aacatcttgc tctcctggtt ggcgtccacc
      661 acgaagtcgg atacgccgta agtaccccac agcgcctcct ccgcgagcct gatcgcggtt
      721 tcggtgacta tggatgtgga acccacccag ggaatggtga cgccgagttg gcgcaattgc
      781 ctggcgaaaa tgcccacgtc cggcgcattg gtcatatagc tgccgatcac attcgcgccg
      841 gatttcttca ccgcgagcac gatgggggtg aagtcctgct ggttgttggt gtagccctgc
      901 accagcacag gctcgatccc gtacgccttc aacgcgtcta ccagcgcatt cttgccgccc
      961 gtgccgaagg tatccgtcga gtggatgatc gcccactttg ctccgttgag cttcttgacc
     1021 ccgaactctg ccattacttt cgaggagtac aggtcgttcg gacgaacacg gaaaatccat
     1081 ggattgttca cgcgggtaag gctgggatcg ctgcctccga tcagggccgg aatgcgcgcc
     1141 ttggcgatgg tgggggagac tgcctgcacc tgcgtgctgc gcagcggacc gatcactgcc
     1201 acaactttct tgcctaccag cttggagtag gccagcaccg tgcccggatt ggtgctctgg
     1261 ttatcctcga tttgaagctc caccggccgg ccaagaatgc cgccgctggc attaatctct
     1321 tcctgcgcca gcctggcgcc gtaggcggtg agcatcccgg cttccgcctg cggacccgtg
     1381 acttcgtcca ccaagccgaa cacgatcgga tcggcggcct gggagggtac ggccgtggca
     1441 agcacgacga cagaagatgc aagaaaaccg gctattgcgt tacgcatgct gctcctcccc
     1501 ttatttgcct tgttgactgc tgcatccgca ccaccggata ttctgccctc aactgcgacc
     1561 cgtgtggaac tgcccccgac cgggcgggcg ctacggggtc gattcggagc gcctaacggt
     1621 tcttccgccg cgacggtacg cgcaacctgg cggccttgcg ggcgtgccct tcgaaaaaca
     1681 cggcaagcag atcgacatcc gcggcgcgcg cgccggtcgg tcgcgccacc agcgcgacgg
     1741 tgcgcgccgg cacctgggtt gcgaacgggc gcgcgatcag ttgggtgccg ttcagtattc
     1801 ccgccttcag tgtgagctcc ggcagcagcg tcacgcccag tcctccctcc accatctgga
     1861 tcagcgtgtg caggctggtg gcttccacca ttgccttcgc cttggctcca cgggccccgc
     1921 aggccgcaat ggcatgatcg cgcagacagt gaccttcttc cagtagcacc acgctgccgg
     1981 gttcgaggtc gcgcaccagc acctccttgc tcttggcgtg cggatcgtcc tggcgcgcca
     2041 cgaaccagaa ttcgtcgcgg aacaatggcc tgacggtcaa ctccccggtg tcgaacggca
     2101 acgcgatcaa agtggcatcg agctcaccgg cgcgcagctt ttccagcaac cgctcggtct
     2161 ggtcctcacg caaataaagc ttcagctccg gataggcttg tctcagggcg gcaagcacac
     2221 ccgacaacag gaaaggcgcg atcgtcggga tgacgccgag ccggaacagt cccgacagcg
     2281 gcgcaccggc gccgcgcgcc gtctccacca ggtcctgggc ggcggcgagc agggtgcgcg
     2341 cgcgacgggt gacttcctcg ccggcagggg tgaggcgcac gccacgggga tcgcgttcga
     2401 ccagctccac acccagcagc gtctccagtt ccttgatgcc ggctgacaga gtggactggg
     2461 tcacgaagct ggcttcggcc gcggcgcgga agttgcgctt gtcggcgacc tccaccaggt
     2521 agtgcagctg gcgcagcgaa ggcaatgcgg ccatgccgtc tccatgatcg aataattcga
     2581 tcagtttaaa cgaatttact cattggatcg attggaccgg ccggcgcaga ctgcgcttac
     2641 ggtacttttt caacagaaac tgaaaggaaa cctccatgct caccattggc gacagactgc
     2701 cggcttttga cctggccgcg gtcgtttcga ccgaacagga caaggccttt acccgcatca
     2761 ccggggcgtc caaccccggc aagtggaaag tgctgttctt ctggccgaag gacttcacat
     2821 tcgtgtgtcc gaccgaaatt gccgcgttcg gcaagctgaa cgccgacttc aacgatcgcg
     2881 acgcgctgat ctacggtgtg tcgatcgaca gcgaattcgt gcacctggcc tggcgcaaca
     2941 accacgctga cctcacggac ctgccgtttc cgatgctggc cgacatcaag cgcgaactgt
     3001 cgcaggcgct gggcgtgctg gacaagaagg aaggcgtggc tctgcgcgcc accttcatcg
     3061 tcgatcccga gggcatgatc cgcttcgtgt cggtgaacga catgaacgtc ggccgcaacc
     3121 ccgaggaagt gctgcgcgtg ctggatgcgc tgcagaccga cgaactgtgc ccgtgcaact
     3181 ggaagaaggg cgacgagttc ctcaaagccg cctgagccgg ctttccggac gcagtacgaa
     3241 accccgaacc acggaggaca cggaggacac ggagaaaaac gaggaaaacg aactggggat
     3301 agaagcgggg taactgttcg caatccaatg ccgccaattt tggctttgtt gcagcatccg
     3361 ccaatcggag attttcaccc tcgtttgttt tcttgctctt gcctttcccc cgtgtcctcc
     3421 gtggttcgga aatcagcaat acaaagaaag gattcaacca tgtcgatcca gaccctgaaa
     3481 gaccggctgc cggaatatgc caaggacctg aaactgaacc tgggctcgct tgccgccgag
     3541 accgttctca acgataccct cagggcaggc accttcgtcg ccgcggcctt tgcctcgcgc
     3601 aacgccgagg tgatcgccgc cgtgaacgcg gaattcgccg gcaagctggc gccagccgat
     3661 ttaaccgctg ccaaggccgc agccgcgatc atgggcatga acaatgtcta ttaccgcttc
     3721 ctgcacctgt cggagaacgc ggagtaccag cgcctgccgg ccaagctgcg catgaacgta
     3781 atcgccgcac cgggcaccgc cagggagaac ttcgagttgt ggtcgctggc cgtttctgca
     3841 atcaacggct gcggtctgtg catcaccgcg catgacaagg ttgtgcgcgc cgccggcatc
     3901 actgcggaac aagttcaggc tgcggtgcgg attgccgcgg tggtacatgc cgtcgccgtg
     3961 acactggaat cggaagcagc ggcaaacgtg gaacttgctg cagcagcgta accccccact
     4021 tcacccattc ccgctccccc tttactgaag ggagtcggaa aaagtaaaag aactgaaacg
     4081 ggcgtccgca cggtcgcccg tttgcattta tccctccctc tccgcccttt ggggggagag
     4141 ggccggggag aggtgggtgt ctggttcgaa tccaacttcc ttcatgtaga gcgccaagac
     4201 gttgtccatg ctggccgcat gattggcgaa ccactccgcc acagccttgg ccaatacgcg
     4261 gccgaagccg gtttcgcccg cggctgcgcg tctgtgcact tcgcgtgcta tttccagaac
     4321 accgtcgtgt tccctcacgt ggcattcggc gggcggaaag gacatctgct ccatccaccg
     4381 ctgctcctgg gaaaagtgcg cttcggtgtg cgcaatgaac tcgtcgagca ctgccagcgt
     4441 ctcgctatcc ggcgcatcgg ccagccggtt caacaggccg atgaattcgc ggtgggtgtc
     4501 gtccatgact ccctggtcga gcaccaggtt gtcgtcccat tccattaccg gcatgaggtc
     4561 tcctgcggag aagcagcgaa tgccgcagtc tagcgcatca ttccccagcc ggttcggggt
     4621 cggcggcacc gagtctgcgc ccagcacgct tcgattgaca gagccgatgt gccgtaagac
     4681 cctgcacatg tatggggcct acgcgtccgg atgaagaatt tgcccgatgc ccgaacttcc
     4741 cgacatcgtc gtctacatcg agaacctgga atccaggatt cagggcagaa tcctcgagcg
     4801 catccggcta ctgaatcctt ttgttctgcg caccgcgatc ccgccaatct cgtcggccga
     4861 gggcaggaag gtcctgggcc tgaggcgact gggcaagcgc atcgtcatcg gactggaggg
     4921 cgatcttttc ctggtgctgc atctcatggt tgcgggcagg ctgcgctggc tcgaaggcga
     4981 ggcgaggctt cccggcaaga tcgcgctggc cgcggtcgaa ttcgacggcg gaacgctggt
     5041 attcaccgaa gccggcacca aacgccgcgc ttcgatccat ctggttcagg gtgaagctgc
     5101 gatgaacgcg ctgcatgccg gcggaatgga agtgctggag tcggggcagg cggccttctc
     5161 gcaacggctc aaggctgaaa accacaccct caagcgcgcg cttaccgacc cgcacatctt
     5221 cagcggcatc ggcaacgctt attccgacga gatcctgcac cgcgccagac tctccccgat
     5281 cgcgttgacc gggacactgg gcgacgagga ggttttacgc ctgtttgagg ccacgcgttc
     5341 cgtattggga gagtggaccg accgactgag gaaggagacc ggcggcgggt ttccggaaaa
     5401 aatcaccgcc ttccgcgagg gtatggcggt gcacggccgt tatggcaagc cctgcccggt
     5461 atgcggttcg ccggtgcagc gaatcgtcta cgccgacaac gagactaact actgtgcccg
     5521 ctgccagacc ggcggcagga tcctcgccga ccgtgcactg tcgcggctgc tcaagaaaag
     5581 ctggccgcgt tcgctggagg aaatcgagtg aatcggcgct tctacgcctt tagcgagatt
     5641 tcgggcaatg atgccgaacg ggtccccggc aacggccggt tcccggtact gtgataggtg
     5701 aacaccgcgg cgaacttggt gaggccggtg cggttcctgc cggcggcatg cagcaggcgg
     5761 cagtgaaaga acagcacgtc tccctcgtct agttccgcgg tcatccgtcc gtcgaggatc
     5821 ctgcggttct cctcgacatc ttcgcgcagg aaaagcgcgt catcgaactg ctcgcgccgg
     5881 aactcgatcc ggtgcgaacc cggtatcagc agcagacaac cgttttcctc gtgttcctgc
     5941 ccgagcgcca gccacgccga aatcagttcc ggtttttcaa aagaccagta acggatgtcc
     6001 tgatgccagg aagtcgcgct gctgaacgcg ggattcttgg tcatcacgca attgtgatgc
     6061 gcttgcgaca gctccacctg aggacccagc aacagtctta gccgaccccc gatcggacgc
     6121 gaagttgccc agtccccgaa caccggatcg cgggcgtaag cctgcaacag ccgtcgcacc
     6181 gtcttgccgc ccggtgcgtc gagcgacacc ggtgaaccgg ggtacttcgt ctgggcttcg
     6241 tactcgaccg gctggacagc ctgcgcaagg tgccgctgcg cgagtgactt catccgctca
     6301 cacagttcat gcgccgcaag gtttctcgcg atcacgaaac cattgctctc gaagtattcg
     6361 acctcagagg gggaaaatac cgacacggaa tttcagaaag aaaggaggcg ttgcgagcaa
     6421 ccaacccgaa ttgtacgact tgccgaagac ctcgcggatc agcgttctgg gcgtcgggta
     6481 ctggcgagac gcgtttaccc ttcacccttc acgcattcac ccttcaccgc cgctcagcgg
     6541 tgtgcggcgt tgaacttctc cagcgcttcg acaccgggac agagatcgcg ttcggccagc
     6601 ctgttgaacg gcgtctcaac ggtctccagg tgcgcgtgca gatcttccgg atcggggtcc
     6661 acgtgcagca ccccggtcac gatttccccg gccgccttgc gctcctgcag gtaattcatc
     6721 gcagcaatgc ggtcgtgcac gtcgtactcg gcgtggagct tgcgcaaccg cagcaccgag
     6781 ccgtcgtgct gcgtgacttc ttcaacctcg cctggcgcgt agtcggcggt gatagattcg
     6841 cgcggcatca tgacatcgag ccggttgacc gcctcattgt gctcgcgcac gtaatcgaaa
     6901 cccttggtcg agccgacgtg gttgttgaac gccacgcacg gcgagatgca gtcgatgaac
     6961 gccgcgccgc ggtgcacgat cgcccccttg atcagcggca ccagttgcgt cttgtcgccg
     7021 gagaaaccgc gtgccacata agtggcgcca agttccatcg ccagcatcac catgtcgatc
     7081 gcggcgtcgg tattcaatgc gcctttcttg ctcttcgagc cccggtccga ggtggcggag
     7141 aattggccct tggtgagccc gtacacgccg ttgttctcga cgatgtacac catgttcacg
     7201 ccgcggcgca ggctgtgggc gaactggccg agcccgatcg aagccgaatc gccgtcgccg
     7261 gaaacgccca ggtagatcag gtcgcggttg gcgaggttgg cgccggtcag caccgagggc
     7321 atgcgcccgt gcacgctgtt gaacccgtgc gaactgccga gaaagtaggt cggcgtcttc
     7381 gacgagcagc caattcccga gagcttggcg atccggtgag gaggcagatc cagctcgaag
     7441 caggcctgaa cgaccgccgc gctgatcgag tcgtgtccgc aaccggcgca caacgtcgag
     7501 atcgcaccct cgtagtcgcg ccgggtgtag ccgagcttgt tgagcgcaag ctgggggtga
     7561 tgaagtctgg gcttggcaat gtaggtcata gaaattcagg cgctagcctc taggcacgag
     7621 gcgcgaggaa ggacacagca tgttttgctc tcatttcgat tgctcccgat atttgatcaa
     7681 accgccaagc attcgagaca cttcttcaca actggcgatc aatcgacttg cgtcacccga
     7741 ctccaggtat ccaacccccg tagcgagatc ggcctgcgac atcagttcca
//
LOCUS       DASQOP010000119         3272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2666015, whole genome shotgun sequence.
ACCESSION   DASQOP010000119 DASQOP010000000
VERSION     DASQOP010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2666015"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..634)
                     /locus_tag="VE030_04685"
     CDS             complement(164..634)
                     /locus_tag="VE030_04685"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HZF22750.1"
                     /translation="MKKIIIAFAMVMALFSAGAMAQGTQGLMFGGKAGFQKPHGSDND
                     AGFSIGAVLGKPIQGELSWEAELMLGAIDGKIGNQDWSINSVAGYAVYRSQGDVHVKA
                     KLGVSYWDDDIDNDVNLTAGIGLGFRIGRGILDVEYTQVNPRTDYITVGFIMPF"
     gene            complement(898..2379)
                     /locus_tag="VE030_04690"
     CDS             complement(898..2379)
                     /locus_tag="VE030_04690"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HZF22751.1"
                     /translation="MVECAGSCACSPVLPGHKVRAMTQEIVLYETVANSISGMISAGT
                     LRPGERVPSVRRLSNQRRVSIATVLRAYEVLENRGLIEARPNAGYYVRHRSPPVGEPS
                     ISNPPAAPHLVGISALVQKVLDSRRPGVVSFGAACPGPDHMPTAKLQRLISSIARRKP
                     LSLTHYVLPPGNEELRRQIARKSLDLGCNLAAADIIVTHGCMEALNLCVRAVAKPGDT
                     IALESPTYFGLLQILESMGVKALEIPTHPREGISLEALEFALDRSRIAAVIAMPNAQN
                     PLGFTMSDDNKKRLVKLLDKRGVPLIEDDVYGDLHYGDERPLPAKAFDRSGNVMLCSS
                     FTKTVAPGFRLGWVAPGRWHANVQMLKFINSVGCAELLQLAIAEFLANGGYDRQLRSL
                     RRVFREQVSHVSAAVSEHFPSGTRITRPAGGFILWVELPEGCNSEELFQQAIRNNISF
                     GPGVLFSATDRYRNCIRMGCAEPWSPRIEQAIIKLGGLAQRQL"
     gene            2460..2753
                     /locus_tag="VE030_04695"
     CDS             2460..2753
                     /locus_tag="VE030_04695"
                     /inference="COORDINATES: protein motif:HMM:NF022592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HZF22752.1"
                     /translation="MTTMKVEIENAAIELEPAQWLRLKGAAGVRIVCRSGTVWVTQEG
                     VLRDDFLCSSEALTLRSRGVTLVQAEGRALISIEAGPRIGAATATLSALPVAG"
     gene            complement(2848..>3272)
                     /locus_tag="VE030_04700"
     CDS             complement(2848..>3272)
                     /locus_tag="VE030_04700"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HZF22753.1"
                     /translation="FVQKHYESGNMQGLQARYTQKGVVWLGINSTSPRHSNYRDPARS
                     QAILKEWKSAPTAFVLDPDGKVGKQYGARTTPHMYVIDPKGTLVYVGGIDDRPSTSQR
                     DIATARNLVAAALDESLSGKPVGTPSATPYGCSVKYDN"
BASE COUNT          615 a          967 c         1054 g          636 t
ORIGIN      
        1 ccggcgtgtt atttacgcgg tgatctgggc gatcgagctg ccgacatcgg cggctgatct
       61 gcttgatcgg tagaaaaaaa cagcccgcgc atcggatgat gcgcgggctt tttctttggc
      121 cgggcgcgcg attggcccgg agcgggtacc agcgattccg gaatcagaac ggcattatga
      181 agccgacggt gatgtagtcg gttcgtgggt tgacctgggt gtattcgacg tcaagaatac
      241 ctcgccctat acggaagccc agaccgattc cggcggtcaa gttgacatcg ttgtcgatgt
      301 cgtcgtccca ataggaaacg ccaagcttgg ccttgacgtg aacgtcgccc tggctgcgat
      361 acaccgcgta gcctgcgacc gagttgatgc tccaatcctg attgccaatt ttaccatcga
      421 tggcgccgag catcagttct gcttcccacg acagctcgcc ctggatcggt ttgcccagca
      481 ccgcgccgat gctgaatccg gcgtcgttgt cggagccatg cggcttctga aagccggctt
      541 tgccgccgaa catcagtccc tgagtccctt gcgccatcgc cccggccgag aacaacgcca
      601 taaccatcgc gaaggctatg atgattttct tcattggatc tcctcgttgt gtagtggaac
      661 agaaaacaca ggcagattcc gccacctgtc gagattgccc agggacccgc ggtttccggt
      721 cgatgcgcgg cacgccccgg ctgaacatcc gcaggcattg tgtcattccg tccgatgatt
      781 gaaaactgtc gccatcgggc gacagttgcc aatgcaattc ggcgtgcgct ggccgcgaag
      841 tgaattgccc gcgcggattg ccaggttggg ggaagtgtcg tcgcgccggc tccgacctta
      901 gagttgccgc tgtgccagtc cgccgagctt gatgatcgcc tgttcgatgc gcggcgacca
      961 gggctcggcg cagcccatgc ggatgcagtt gcgatagcgg tcggtggcgg agaacaatac
     1021 gcccggcccg aacgagatgt tgttccggat ggcttgctga aacaattctt ccgaattgca
     1081 gccttccggc agttcgaccc agagaatgaa gccgcccgcc gggcgggtga tgcgcgtacc
     1141 ggaaggaaag tgctccgaga ccgcggcaga aacgtgggac acctgctcgc ggaacacgcg
     1201 ccgcagactg cgcaattgcc ggtcgtaacc gccattggcg aggaactccg caatcgccag
     1261 ttgcaacaac tcggcgcagc cgaccgagtt gatgaatttc agcatctgca cgttggcgtg
     1321 ccagcgaccg ggcgccaccc agcccagccg gaaccctggt gctacggtct tggtgaacga
     1381 ggagcacagc atgacattac cgctgcggtc gaaagccttg gccggaagcg gccgctcatc
     1441 gccatagtgc agatcgccgt acacgtcgtc ctcgatcagc ggcacgccgc gcttgtccag
     1501 caatttcacc aggcgtttct tgttatcgtc ggacatggtg aaaccgagcg ggttctgcgc
     1561 attcggcatg gcgatcacgg ccgcgatcct ggagcgatca agcgcgaact cgagcgcctc
     1621 cagcgagatg ccttcgcgcg gatgggtggg aatctccagc gctttgacgc ccatgctctc
     1681 gaggatctgc agcaggccga aatacgtcgg cgactccagc gcgatggtat cgccgggctt
     1741 ggcaaccgcg cgcacgcaga gattgagcgc ctccatgcag ccgtgggtca cgatgatgtc
     1801 ggctgcggcg agattgcagc cgaggtccag agacttgcgc gcaatctggc ggcgtagttc
     1861 ctcgttgccc ggcggcagca cgtaatgcgt gagcgacaac ggtttgcggc gggcgatcga
     1921 agagatcagg cgctgcagct ttgcagtcgg catatggtcc gggccagggc aggcggcgcc
     1981 gaaggacacc acgccgggcc ggcgcgaatc cagaactttc tgcaccaggg cgctgatgcc
     2041 aaccaggtgc ggcgcagccg gcgggttgga gatggacggc tcgccgaccg gtggcgagcg
     2101 atggcgtaca tagtagccgg cgttcggccg cgcctcgatc agcccgcggt tctcaagcac
     2161 ttcgtaggcg cgcaggaccg tggcgatcga gacgcggcgc tggttggaca ggcgccgcac
     2221 tgaaggcacg cgctcgccgg gccgcagcgt acccgcggag atcattcccg agatcgagtt
     2281 ggcgacggtt tcgtaaagga cgatttcctg agtcatggcg cgcactttat ggccgggcag
     2341 cacgggcgaa caggcacagc tgccggcaca ttcgaccata acagttttct gtttggcaaa
     2401 ctgttatgta cacaatttca gatttctgaa tctgttatgg ccaatatcgg ggccctaaga
     2461 tgaccaccat gaaagtggaa atcgaaaacg ctgccatcga actcgagccg gcgcagtggc
     2521 tgcgcctgaa aggcgcggcc ggggtgcgca tcgtctgtcg cagcgggacc gtgtgggtga
     2581 cgcaggaggg tgtgcttcgc gatgattttc tgtgctccag cgaggcgctg accttgcgca
     2641 gccgcggcgt gacgctggtg caggcggagg gtcgcgcatt gatttcgatc gaagcggggc
     2701 cgcggatagg cgccgcgacc gctacgctgt ctgcattgcc cgttgccgga taacggtcaa
     2761 gtcaaaatca cagatctcac cacgaaggcg cgaaggacac gaagaagtac aagatcagaa
     2821 aaaaactgaa ttccgtacct gaaatcgtca gttgtcgtac ttcaccgagc agccgtaggg
     2881 cgtggcgctg ggcgtgccga ccggcttgcc gctgagtgac tcgtcgagcg ccgcggcgac
     2941 gaggtttctt gcggtggcga tgtcgcgttg gctggtcgag ggcctgtcgt cgattccgcc
     3001 gacgtagact aatgtgcctt tcgggtcgat cacgtacatg tgcggcgtgg tgcgggcgcc
     3061 gtactgcttg ccgaccttgc cgtcgggatc gagcacgaac gcggtgggcg ccgacttcca
     3121 ctccttgaga atagcctgcg aacgcgccgg gtcgcggtaa ttgctgtgcc tgggactggt
     3181 tgaattgatt cccagccaca ccacgccctt ctgcgtgtac ctggcctgca gcccctgcat
     3241 gtttccgctt tcgtagtgct tctgcacgaa cg
//
LOCUS       DASQOP010000120        16535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2469660, whole genome shotgun sequence.
ACCESSION   DASQOP010000120 DASQOP010000000
VERSION     DASQOP010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 16535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16535
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2469660"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..130)
                     /locus_tag="VE030_04705"
     tRNA            complement(57..130)
                     /locus_tag="VE030_04705"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(96..98),aa:Cys,seq:gca)
     gene            complement(279..353)
                     /locus_tag="VE030_04710"
     tRNA            complement(279..353)
                     /locus_tag="VE030_04710"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(319..321),aa:Gly,seq:gcc)
     gene            complement(413..982)
                     /gene="pgsA"
                     /locus_tag="VE030_04715"
     CDS             complement(413..982)
                     /gene="pgsA"
                     /locus_tag="VE030_04715"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HZF22754.1"
                     /translation="MPINLPNLLTWLRILMIPLLVGVYYVPHEWIADHDKNLVATAVF
                     SFAAITDWLDGYLARTLNQTSSFGAFLDPVADKLMVATALIMLVWIGHLEGFIAVIII
                     GREIAISALREWMARIGKSKSVAVSFLGKVKTVSQMAAIIMLLYHDEFLTLSMHAIGT
                     ILMYVAAVLTLWSMVYYLKIALPQAMKSG"
     gene            complement(990..2831)
                     /gene="uvrC"
                     /locus_tag="VE030_04720"
     CDS             complement(990..2831)
                     /gene="uvrC"
                     /locus_tag="VE030_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HZF22755.1"
                     /translation="MKSDPELPRTAGPAFDPDEAVASLPHLPGVYRMLNAAGEVLYVG
                     KARDLKKRVASYFQKTGSLAPRILLMMTQVGGLETTVTRSEAEALLLENNLIKSLSPR
                     YNILYRDDKSYPYIVLTGHQFPRLGFHRGTLDKIHRYFGPFPNAGAVRESIQLMQKVF
                     RLRTCEDTVFANRSRPCLLYQIKRCTGPCVGLISETDYSEDVRSAELFLQGREDEAAD
                     RLEERMHRAAAEQAYEQAAGYRDQIRSLSKVRETQFVTSSRSVDADIVALAATGGLIC
                     VNLVMVRGGIHRGDKSFFPQNAQDQDEQIALEAFLTQHYVNRDVPPAIIVNRPVEAAA
                     LEELLSQQAGTKVQINANPAGERRIWLRMAEKNAELAIAQRLAAHATQETRLTALQQA
                     LGLADNVRRIECFDISHTMGEATVASCVVYDGAAMQPGEYRRYNINGVEPGDDYGAMR
                     QVLDRRYRKIVEGEGKLPDLILIDGGKGQVSTAFGVLAELGLSDLPMIGVAKGEERKA
                     GLEQLIFPDRNEPLRLASDHPGLHLIQQIRDEAHRFAISGHRNRRAKARNRSTLEEIV
                     GIGAKRRQRLLSRFGGLKGVASASVEELAQVEGISTGLAEKIYQQLH"
     gene            complement(2892..3557)
                     /locus_tag="VE030_04725"
     CDS             complement(2892..3557)
                     /locus_tag="VE030_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HZF22756.1"
                     /translation="MKTGHTDIFSTDCRRCGRLAHFLDGVRKEFPGYFSRPVPPFGDA
                     EARLLIVGLAPGMHGANRSGRPFTGDYAGLLLYRTLHKFGFANRAQSVHVDDGLLLTG
                     CRITNAVKCLPPENKPALDEVKRCNAYLAAELAVLPTGAAVLALGGIAHKAVLMAAGE
                     KAANFKFAHGAQHPLECGLTLFDSYHCSRYNTQTRRLTEPMFEQVFAAIGRFFQDPAA
                     CRS"
     gene            complement(3609..4550)
                     /locus_tag="VE030_04730"
     CDS             complement(3609..4550)
                     /locus_tag="VE030_04730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HZF22757.1"
                     /translation="MASHAPQHHHSHGAGRTLLIALAVTLGYAVVEFVGGRIFGSLAL
                     MSDAGHMLSDSLALGLAAFAARVAARPAGVRHSYGFARAEVVAAFVNSLAMLLIVVLI
                     VVEAVARLLEPRPVAGLGVMVVAFCGLVVNLGVAFLIGRGEHNMNTRAALVHVVGDLI
                     GSVAAITAGAVIYFTGWQPIDALLSVVIALLILASTVGLSRDALHVLMEGVPAGVRLE
                     EVGQALSQIEGVRAVHDLHVWNISSGQVALSAHVDLEDLANWPVLLESARRMLQARFR
                     IAHVTLQPELAGGINPGYRARVKIVPHREEHRHKDGK"
     gene            complement(4543..5601)
                     /gene="nagZ"
                     /locus_tag="VE030_04735"
     CDS             complement(4543..5601)
                     /gene="nagZ"
                     /locus_tag="VE030_04735"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HZF22758.1"
                     /translation="MLDLEGKALTREDRGRLLHPSTGGVILFSRNYESPRQIAELTAE
                     IHAVRDPPLLIAVDHEGGRVQRFREGFTLLPPMRALGEIWDQHPQRARHLAHAAGYVL
                     AAELRACGVDLSFTPVLDLDFGSSAVIGNRAFHGDPQAVCELAVALVEGLRQGGMAAV
                     GKHFPGHGFVAADSHTDVPVDSRGYAEIEQADLIPFQHLFRHGLSAVMPAHVIYAKVD
                     SKPAGFSSVWLSQVLRGQMRFQGVIFSDDLSMEGARVAGTVVERAQAALDAGCDMVLV
                     CNNPLAADELLAGLGRKASPMSLARLARLHGRAQAPTLTALKETEPYTRAVHAIAGVG
                     FSSGELPLAGTPRVGERG"
     gene            complement(5639..6016)
                     /gene="acpS"
                     /locus_tag="VE030_04740"
     CDS             complement(5639..6016)
                     /gene="acpS"
                     /locus_tag="VE030_04740"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HZF22759.1"
                     /translation="MIIGIGVDLADAVRIERMLVEYGSRFAERILAPSECAAYRISSR
                     PIWFLANRFAAKEALSKALGTGLRFPVTLHAISVQSDAIGKPEFQFHGALPEYLAGRG
                     VVRCHLTLSHEKDLACAMVVVEG"
     gene            complement(6013..6753)
                     /locus_tag="VE030_04745"
     CDS             complement(6013..6753)
                     /locus_tag="VE030_04745"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HZF22760.1"
                     /translation="MTRLSVNVNKVALLRNSRPLDIPSVVRAAEIAIDAGAHGITVHP
                     RPDARHIRPADVHSLHKLARARGVEFNIEGNPFHPMLELVRAVRPDQCTLVPDDQDAL
                     TSDHGWDLGRNGARLRPVIAELKTLGVRVSLFMDAEEQAMAAARAVGADRVELYTESY
                     ARAFGKTEQAPVLARYAAAARAAQREGLGVNAGHDLNRDNLPQFLRAVPGVLEVSIGH
                     ALIADAIEFGMKQTVALYLGAVSGGRAA"
     gene            complement(6750..7484)
                     /gene="recO"
                     /locus_tag="VE030_04750"
     CDS             complement(6750..7484)
                     /gene="recO"
                     /locus_tag="VE030_04750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HZF22761.1"
                     /translation="MATVSGDRQRQDGQPAFVLHTYPFRETSLVVEVFTRNAGRVGLV
                     ARGARRPRSALRGLLMAFQPLLLSWAGRSELRTLHKAEWQGGTPQLRGAELMCGFYLN
                     ELLLKFLARDDPHDALFTAYHDAIVALAQRLEPAVTLRRFEKHLLKELGYAPTLDREA
                     DTGRPVEPQKRYTYVIERGPVALNGRDGARVEVTGKTLLDLACDNFTDPGTLAQSKAL
                     MRFLMNHYLDNQELHTRQLLRELQQI"
     gene            complement(7489..8379)
                     /gene="era"
                     /locus_tag="VE030_04755"
     CDS             complement(7489..8379)
                     /gene="era"
                     /locus_tag="VE030_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HZF22762.1"
                     /translation="MNASVHRSGYVAIVGRPNVGKSTLLNRLVGTNVSITSRRPQTTR
                     HRITGIVSEPDVQLVLVDTPGFQTAHGGALNKLMNRVVTDSMEGVDAVLFVVESGRFG
                     ADDRKMLSLLPQEIPVVLVINKIDRTKDKSRLLPFIARVSTAFDFAQVVPVSAAKGTQ
                     VGRLLDAVKPYLPEGPAQYGEDEVTDRPERFLAAELLREKLFRLLGEELPYAATVVVE
                     KFETETGLRRIHAAIVVDKPNHKAMVIGKGGAKLKDIATQARVDMEKLFGGKVFLEVW
                     VKVKGGWADSLKALKNLGYE"
     gene            complement(8403..9050)
                     /gene="rnc"
                     /locus_tag="VE030_04760"
                     /pseudo
     CDS             complement(8403..9050)
                     /gene="rnc"
                     /locus_tag="VE030_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011851961.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
     gene            complement(9075..9428)
                     /locus_tag="VE030_04765"
     CDS             complement(9075..9428)
                     /locus_tag="VE030_04765"
                     /inference="COORDINATES: protein motif:HMM:NF027463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HZF22763.1"
                     /translation="MKRQQGVTFVGMIFIATLIVLGAIIALKLVPAYIEYATVVQHLR
                     EIARSPEARGSPREIQTLFSKRAQIDDIKAITANDIEVTRDGDSVVLTAVYQTKVKLF
                     GNLSACIDFEASSSK"
     gene            complement(9440..10243)
                     /gene="lepB"
                     /locus_tag="VE030_04770"
     CDS             complement(9440..10243)
                     /gene="lepB"
                     /locus_tag="VE030_04770"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HZF22764.1"
                     /translation="MNFALLMLVLLLVTGAVWLLDIFVLRKRRGRDAREPWWVEYPKS
                     FFPIILLVFLLRSFLVEPFKIPSGSMIPTLLVGDFILVNKFTYGIRLPVINKKIVDIN
                     QPGRGEVMVFRYPENPTLDYIKRVVGLPGDHVEYRNKRLSINGKLVPVEAAQDFQYVD
                     GGLSFVNAVRFWETLDQHRHYIIVNPEAPPLQLAGVKQFPQRENCDYNEREFSCVVPP
                     GHYFMMGDNRDSSSDSRYWGFVPEENIVGKAFLIWWNFGELKRIGNRIN"
     gene            complement(10279..12072)
                     /gene="lepA"
                     /locus_tag="VE030_04775"
     CDS             complement(10279..12072)
                     /gene="lepA"
                     /locus_tag="VE030_04775"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HZF22765.1"
                     /translation="MQHIRNFSIIAHIDHGKSTLADRIIQLCGGLSDREMEEQVLDSM
                     DLERERGITIKAQTAALRYRARDGQQYHLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIEQGVEVVPVLNKIDLPSADPDRVISEIEDIIGIPAKEAVR
                     ASAKTGEGVEDILDAVIARIPPPRGTLAAPLKALIIDSWFDNYVGVVMLVRVVDGTLK
                     PKDRLLLMSNKSTYLCEQVGVFTPKSQSRDSLSAGEVGFVIAGIKELQAAQVGDTVTL
                     ADEPASAPLAGFKEIKPQVFAGLYPVESNQYEALRDALEKLHLNDSSLHYEPETSQAL
                     GFGFRCGFLGLLHLDIVQERLEREYDMALITTAPTVVYQVLMRDGTVVQIENPSKLPD
                     PSRIDEIREPIITATILMPQEYVGPVMTLCNNKRGAHKNMQYMGRQVMLTYELPLNEV
                     VMDFFDKLKSVSRGYASLDYEFREFRAADLVRLDILINGEKVDALSLIVHRSNSQYRG
                     RELASRMRELIPRQMFDVAVQAAIGSHIIARETVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKKRMKQIGTVEIPQEAFLAILQVESK"
     gene            complement(12191..13576)
                     /locus_tag="VE030_04780"
     CDS             complement(12191..13576)
                     /locus_tag="VE030_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HZF22766.1"
                     /translation="MSRKALLAFCLLLFVPLAGVAQVQGLPDFTELVEKQGPAVVNVS
                     TTSAARSGPQSPVPEDDPFYDFFRRFGPPQPRDYEARSLGSGFIISADGYILTNAHVV
                     EAAEDITVKLNDKREFKAKVIGSDKRTDVAVIKIEASGLPAVKIGDPERLKVGEWVLA
                     IGSPFGFESTVTAGIVSAKGRSLPQENYVPFIQTDVAINPGNSGGPLFNLKGEVVGIN
                     SQIYSRTGGFMGLSFAIPIDVAMDITHQLRTAGRVSRGRIGVVIQEVTKELAESFGLP
                     KAAGALVNSVEKGSSADKAGLEPSDVILKFDGKTVNSSADLPRMVAQTRPGSKVTMQI
                     WRKGAAKDVSVTVGEMPEEKVAQRAGRRDSKGGNVVARLGLTVSELSAEQRKELGIGG
                     GLLVEDVQGAAAKAGIRRGDVLMALNNQDIKSVEQLTQLLNQYDKAKSVALLIRRGDG
                     ALYVPLRLDGN"
     gene            complement(13630..14616)
                     /locus_tag="VE030_04785"
     CDS             complement(13630..14616)
                     /locus_tag="VE030_04785"
                     /inference="COORDINATES: protein motif:HMM:NF015825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HZF22767.1"
                     /translation="MTARSALLLPALFGLGFSAGVALADPLQETEALAMLQKIADAAR
                     ELNYAGTFVYQHSDQVETSRIVHFADASGEYEKLETLDGPRREIIRNNDEVMCYYLDA
                     KVMRSEKRVARRTFPALLPDQLSALTEYYQIRMGQPERIAGFDSQALVLEPRDGMRYG
                     HKFWAETNSGLLLKARMVNERHNLVEQFSFTQLAIGSGISRDMVKPSFSVSFPEWRVD
                     RFAHNLTSNVESGWAVRNFPAGFRKIMEMRRSKLGNSIPVTHMVYSDGLAAVSVFIEP
                     MANRARINEGLSHQGAINIYTRTIDDQVVTVLGEAPASTVTQIANSVSFRGK"
     gene            complement(14616..15125)
                     /locus_tag="VE030_04790"
     CDS             complement(14616..15125)
                     /locus_tag="VE030_04790"
                     /inference="COORDINATES: protein motif:HMM:NF015810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HZF22768.1"
                     /translation="MERISAFMDGELGDHEAASQVRRLKEDPELRTAWETYHLIGDAM
                     RGEAGYSSGLVVKISARLAAEPTILAPRPRIQLRNVRRVALSAAACLGGVALVTWLAL
                     FDSPFAPQQKVAVNQAPPGVQTQLAATPANDAVNDYLLAHQQFSPSTAMQGVASYVRT
                     VSGQNPEQR"
     gene            complement(15128..15727)
                     /gene="rpoE"
                     /locus_tag="VE030_04795"
     CDS             complement(15128..15727)
                     /gene="rpoE"
                     /locus_tag="VE030_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HZF22769.1"
                     /translation="MGDREIDQQLVERAQRGDKHAFELLVAKYQRKLGRLLSRFIRDP
                     AEVEDVAQEAFIKAYRALPSFRGDSAFYTWLYRIGINTAKNYLVAMGRRAPTTTEFDS
                     EEAENFEDGDQLRDLNTPEAELMTRQIAATVNQAMEELPEELRTAITLREIEGMSYED
                     IANVMNCPIGTVRSRIFRARETIADRLRPLLETSKDKRW"
     gene            15952..>16535
                     /locus_tag="VE030_04800"
     CDS             15952..>16535
                     /locus_tag="VE030_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZF22770.1"
                     /translation="MRRFDVAIVGSGLAGLTVALNLADRKKVGIVTKREFLDGASNWA
                     QGGIAAVLAKDDSPEDHIRDTEIAGAGLCNAEVTRYVVERGRQAIEWLIGQGVPFTRD
                     ETSDVGYHLTREGGHSHRRVIHAADATGHAVQTTLEAKVKAHPNITVLDHYMAVDLIT
                     GPKLGQPERRCHGLYALDENAGRVVTIGAEQVVLA"
BASE COUNT         3033 a         5308 c         5037 g         3157 t
ORIGIN      
        1 ccgtgagcgc tcctagactc taggacactt gatgatagac ggctgactcg tgagtttgga
       61 ggcgggggtc ggaatcgaac cggcgtacac ggctttgcag gccgctgcat gaccactctg
      121 ccaccccgcc gggggcacgc agcatgatag ctgcgccgga cacactcttg cagtaatcgt
      181 tgaaaggata gtgctcggtc cgccgcaaga actgcaaaga gaaagcggca agatttaatt
      241 ctggtgaatt aaagctggcc gctttccaca ggggaacctg gagcgggaaa cgaggctcga
      301 actcgcgacc ccaaccttgg caaggttgtg ctctaccact gagctattcc cgcgaaaaac
      361 agagttggca ttatagggag catggccatt ttgtcaagaa aaagggccgt gtttaaccgc
      421 tcttcatcgc ctgcggcaat gctattttca ggtagtaaac catggaccac aaagtcagca
      481 ctgccgcgac gtacatgagg atggttccga tcgcatgcat gctcaaggtc aggaactcgt
      541 cgtggtacag cagcatgatg atagccgcca tctgcgacac ggtcttcacc tttcccagaa
      601 acgacactgc cacgcttttg ctcttgccga tgcgtgccat ccactctcgc aacgcggaga
      661 tcgcgatctc gcggccgatg atgatcacgg cgatgaagcc ctcgagatgg ccgatccaga
      721 ccagcatgat cagcgcggtg gcaaccatca gcttgtcggc caccggatcg aggaaggcgc
      781 cgaaagacga tgtctgattc agcgtgcgcg ccaggtagcc gtccagccag tcggtgatcg
      841 cggcaaaaga gaaaaccgcc gtggccacga gattcttgtc gtggtcggcg atccactcgt
      901 gcggcacgta atacaccccc accagcaacg ggatcatcag gatgcgcagc caggtgagga
      961 gattcgggag gttgatcggc atgagggcgt cagtgcagct gctggtagat tttttcggca
     1021 agtccagtac tgattccttc cacttgcgca agttcctcca cgcttgcgga agcaacccct
     1081 ttcaggccgc cgaaacgcga caacagccgc tgcctgcgct tggcgccgat cccgactatt
     1141 tcttccagcg tcgagcgatt gcgggccttg gcgcggcggt tgcggtgacc ggagattgca
     1201 aagcgatgtg cttcgtcgcg gatctgctgg atcaggtgca aacccgggtg gtccgatgcc
     1261 agccgcagcg gctcgttgcg gtccgggaaa atcagctgct cgaggccggc cttgcgctct
     1321 tcgcccttgg ccacgccgat catcggcaga tcgctcaggc ccaattcggc gagcaccccg
     1381 aaggccgtgc tcacctgccc tttgccgcca tcgatcagga tcagatcggg cagtttgccc
     1441 tcgccttcca cgatcttgcg atagcgccgg tccagcacct ggcgcatcgc gccgtaatcg
     1501 tcgcccggct ccacgccgtt gatgttgtag cgacggtatt cgccgggctg catcgccgcg
     1561 ccgtcgtaca ccacgcacga tgccacggtg gcctctccca tggtatggct gatgtcgaaa
     1621 cactcgatgc ggcgcacgtt gtcggcaagt cccagcgcct gttgtagcgc ggtgaggcgg
     1681 gtttcctgcg tggcgtgggc cgccagccgc tgcgcgatcg ccaactcggc gtttttctcc
     1741 gccatgcgca gccagatgcg gcgctcgccc gccgggttgg cgttgatctg taccttcgtc
     1801 cccgcctgct gggacagcag ttcttcgagt gccgccgctt cgaccggacg gttcacgatg
     1861 atcgccggcg gcacgtcacg gttgacgtag tgctgcgtca ggaaagcttc gagcgcgatc
     1921 tgttcgtcct gatcctgcgc attctgcggg aaaaagctct tgtcgccgcg atgaatcccg
     1981 ccccggacca tcaccaggtt cacgcagatc agccccccgg tggcggccag cgcgacgata
     2041 tcggcgtcca cgctccggct gctggttacg aactgggttt cgcgcacctt ggaaagcgaa
     2101 cggatctggt cacggtatcc cgcggcctgt tcgtaagcct gctccgccgc tgctcggtgc
     2161 atgcgctctt ccaagcggtc ggctgcctcg tcttccctgc cctgtaggaa cagttccgca
     2221 ctcctgacgt cctcggagta gtcggtttcg ctgatcaggc ccacgcaggg tcctgtacaa
     2281 cgcttgatct ggtacagcag acaaggccgc gagcgattgg caaacaccgt gtcctcgcag
     2341 gtgcgcagcc tgaatacttt ctgcatcagc tggatgcttt cgcgcaccgc gccggcattg
     2401 ggaaacgggc cgaagtaacg atggatcttg tccagagtgc cacgatggaa tccgagcctg
     2461 gggaactggt ggccggtcag gacgatataa ggataagatt tatcatctct atacagtatg
     2521 ttatagcgtg gacttaaact cttaattaag ttgttttcca gcagcagcgc ctcggcctcg
     2581 gagcgcgtca cggtggtctc cagtccgccg acctgggtca tcatgagcag aatccgggga
     2641 gccagagacc cggttttctg gaaataggaa gccacccgct tcttcaggtc gcgcgccttg
     2701 ccgacgtaga gcacctcccc cgccgcgttg agcatacggt aaacccccgg caggtgcgga
     2761 aggctggcta cggcctcgtc cgggtcgaac gcgggtccgg cagtccgggg cagttccggg
     2821 tcactcttca tcgggatgtt tcgctgcgac tgtctggccc tggctacccg ccgcgacagc
     2881 aacctggaaa ttcacgatcg gcatgcggca gggtcctgaa aaaagcgccc gatggccgcg
     2941 aagacctgct cgaacatggg ctcggtcagg cgcctggtct gggtattgta tcggctgcaa
     3001 tggtagctat cgaacagcgt caatccgcat tccagaggat gttgtgcgcc gtgcgcaaac
     3061 ttgaagttcg ccgccttctc tccggcggcc atcaacactg ccttgtgagc gatgccgccc
     3121 agcgccagga cggcggctcc cgtcggcagc accgccagct cggcggccag ataggcattg
     3181 cagcgcttca cctcgtctaa agccggcttg ttttccggcg ggaggcattt aaccgcgttg
     3241 gtgatcctgc agccggtaag cagcagcccg tcgtccacgt gcaccgactg ggcacgattg
     3301 gcgaacccga acttgtgcag cgtgcggtaa agcagcagcc cggcgtaatc cccggtaaac
     3361 gggcgaccgg aacggttggc gccgtgcatt cccggcgcca ggccgacgat cagcaggcgc
     3421 gcttcagcat caccgaaagg cggtacgggc ctggagaaat agccgggaaa ttccttcctg
     3481 accccatcga gaaagtgcgc gagccggccg cagcgccggc aatcggtgga gaaaatgtct
     3541 gtatgtccag tcttcatcgt aaccctggtg tccgtgatgc ttgatcgatc gggccagacc
     3601 gtccgccgtc attttccgtc tttgtggcga tgctcctcgc ggtgcggaac gattttcact
     3661 cgcgcgcgat agccagggtt gatgccgccc gccaattccg gttgcagggt cacgtgcgcg
     3721 atgcggaagc gagcctgcag catgcgcctt gccgattcca gcagcaccgg ccagttcgcc
     3781 agatcctcca ggtcgacgtg cgcagacagg gccacctgcc cggaggaaat gttccagacg
     3841 tgcaggtcgt gcaccgcgcg cacgccctcg atctggctca gcgcctgccc tacttcttcc
     3901 agcctcacac cggccggcac accttccatc agcacgtgca gcgcatcgcg cgacagaccc
     3961 accgtggacg cgaggatcag cagcgcaatc accaccgaca gcagcgcgtc gatcggctgc
     4021 cagccggtga aatagatcac cgcgccggcc gtgatcgcgg cgaccgaacc gatcagatcg
     4081 ccgacgacgt ggacaagcgc ggcgcgtgtg ttcatattat gttcgccgcg accgatcagg
     4141 aacgccacgc ccagattcac taccaagccg caaaacgcca ccaccatcac gcccaggccg
     4201 gcgaccggcc ggggttcgag caggcgcgct accgcttcga ccacgatcaa caccacgatc
     4261 agcagcatcg ccagactgtt gacgaatgcc gctacgactt ccgctcgggc aaatccgtaa
     4321 gagtgccgga cgccggctgg ccgggcggcg acccgggcag cgaatgcagc gagacccagc
     4381 gccagggaat ccgacagcat gtggccggcg tcgctcatca gcgccagcga accgaaaatc
     4441 ctgccgccga caaactccac cacagcgtag cctagcgtaa ccgcaagcgc gatcagcagc
     4501 gtgcggcctg cgccgtgact gtggtggtgc tggggcgcgt ggctagccac gttctccgac
     4561 ccgcggtgtt cctgccagcg gcaattcccc gctgctgaag ccaacgccgg caatggcgtg
     4621 taccgcccgc gtgtacggct cggtctcctt caacgcggtg agcgtgggag cctgcgcccg
     4681 gccatgcagg cgcgccagcc gtgccagcga catcggtgag gccttgcggc ccagtcccgc
     4741 cagcagttca tcggcagcga gcgggttgtt gcataccagc accatgtcac aaccggcatc
     4801 gagagcagct tgcgcgcgct cgaccacagt acctgcgacc cgtgcgccct ccatgctcag
     4861 atcgtcgctg aaaatcacgc cctggaaacg catctgcccg cgcaacacct gcgacagcca
     4921 gacgctcgag aagccggccg gcttgctgtc cactttcgcg taaatcacgt gagccggcat
     4981 gactgcggac aggccatggc ggaaaagatg ctggaaagga atcagatcgg cctgttcgat
     5041 ttcggcataa ccgcggctgt ccacgggcac gtcggtatgc gaatccgcgg cgacgaagcc
     5101 atgacccgga aaatgcttgc ccaccgccgc catgccacct tgccgcagcc cctccaccag
     5161 tgccaccgcc agttcgcaaa ccgcctgcgg atcgccgtga aatgcgcggt tgccaatcac
     5221 tgcactgctg ccgaagtcca ggtccaggac cggagtgaaa ctcaaatcaa ccccgcaggc
     5281 gcgcagttcc gccgcaagca catagccggc agcatgagcc agatggcgcg cccgttgtgg
     5341 atgctggtcc catatttcgc ccagcgcgcg catcggcggc agcagagtaa aaccttcgcg
     5401 gaagcgctgc accctgccgc cttcgtgatc caccgcgatc agcaacggcg ggtcgcgcac
     5461 cgcgtggatt tcggcggtca actcggcgat ctgacgcggc gattcgtagt tgcgcgaaaa
     5521 caggatgacg ccgccggtcg acgggtgcag cagccgcccg cggtcctcgc gtgtcagtgc
     5581 ctttccctcg aggtcgagca tgaccgggcc cagcgatgtc ggcgctgcac tcaacgcctc
     5641 aaccctcaac caccaccatc gcgcatgcca ggtccttttc gtgtgacaag gtcaggtggc
     5701 agcggactac gccgcggccg gccaggtact ccggcaatgc gccgtgaaac tggaactcgg
     5761 gtttaccgat cgcgtcggat tgcacgctga tggcgtgcag cgtcaccggg aaacgcagtc
     5821 cggtacccag agccttcgac aaggcttcct ttgccgcgaa gcgattggcc aggaaccaga
     5881 tcgggcggct gctgatcctg tacgcagcgc actcggatgg cgccaggatg cgctcggcga
     5941 aacggctgcc gtattcgacc agcatgcgct cgatgcggac cgcatccgcg agatccacgc
     6001 cgatgccgat gatcatgcgg cgcgccctcc ggaaaccgcg ccgagataga gcgcgacggt
     6061 ttgtttcatg ccgaattcga tcgcgtctgc aataagggcg tgaccgatcg agacctccag
     6121 aacgcccgga acggcgcgca gaaactgcgg cagattgtcg cggttgaggt catggcccgc
     6181 atttacgccc aggccttcgc gttgtgcggc tctggctgcc gcagcatagc gcgccaggac
     6241 cggcgcctgc tcggttttgc caaacgcgcg cgcgtaggac tccgtataca gctcgacgcg
     6301 atcggcgcca accgcgcgtg ccgccgccat cgcctgttcc tctgcatcca tgaacaggct
     6361 gacgcgcacg cccagcgtct tcaattcggc gatcaccggc cgcaagcggg caccgttacg
     6421 cccgagatcc cagccatggt ccgaagtcaa cgcgtcctga tcgtccggaa ccagcgtgca
     6481 ctggtcgggt cggacggcgc gcaccagttc cagcatgggg tgaaacggat taccctcgat
     6541 gttgaactcc acgccgcgcg cgcgcgccag cttgtgcagt gaatggacat cggccgggcg
     6601 gatatgacgg gcgtccgggc gcggatgaac ggtgatgccg tgcgctccgg cgtctatggc
     6661 gatctcggcg gcgcgcacga cactgggaat gtcgagaggg cgcgagttgc gcaacagcgc
     6721 gaccttgttg acgttgacgc tgagcctggt catatctgct ggagttcacg taacagttgc
     6781 cgtgtgtgca attcctgatt gtccaggtaa tgattcatca gaaagcgcat caaggccttg
     6841 ctctgcgcga gcgtgccggg atcggtgaaa ttgtcgcatg ccagatcgag cagcgtcttg
     6901 cccgtcactt ccacccgggc cccgtcgcgt ccgttcaacg ccaccggccc gcgctcgatc
     6961 acataggtgt agcgcttctg cggctcgacg ggcctgccgg tatcggcttc ccggtcgagt
     7021 gtaggcgcat agcccagttc cttgagcagg tgcttctcga aacgacgcaa ggtcaccgcc
     7081 ggctcgagcc tctgagcgag cgcgacgatg gcgtcgtggt aggccgtgaa cagcgcatcg
     7141 tggggatcgt cgcgggccag gaacttgagc agcagttcgt tcagatagaa gccgcacatc
     7201 agctccgcgc cccgcaactg cggagtgccg ccctgccatt cggctttgtg cagcgtgcgc
     7261 aactcggatc tcccggccca ggacagcaac agcggctgga acgccatcag caggccccgc
     7321 aatgccgacc tgggacggcg ggcgccgcgg gccaccagac cgacacgtcc ggcattgcgg
     7381 gtgaacacct ccacgaccag gctggtttcc cgaaacggat aagtgtgcag cacaaaagcc
     7441 ggctgcccgt cctgcctctg cctgtcgccg ctcactgtcg ccacgaattt actcgtatcc
     7501 caggttcttc agcgccttga ggctgtcggc ccagccgcct ttcaccttga cccagacttc
     7561 gagaaagacc ttgccgccga acagcttctc catgtccacg cgcgcctggg tggcgatgtc
     7621 cttcagcttt gcgccgcctt tgccgatgac catcgccttg tggttcggct tgtccaccac
     7681 gatggccgcg tggatgcggc gcaggccggt ctcggtttcg aatttttcca ccaccacggt
     7741 cgccgcatac ggtaactcct cgccgagcag ccggaacagt ttttcgcgca gcagttcggc
     7801 ggcgagaaaa cgctccggcc ggtcggtgac ctcgtcttca ccgtactgcg ccggaccttc
     7861 cggcaggtat ggcttgacgg catccagcag cctcccgact tgcgtgccct tggctgcgct
     7921 gaccggcacc acctgcgcaa aatcgaatgc cgtgctcacg cgggcgatga agggaagcaa
     7981 acggcttttg tccttggtgc gatcgatctt gttgatcacc agcactaccg gaatctcctg
     8041 cggcagcagc gatagcatct tgcggtcgtc cgcaccgaac ctgcctgact cgaccacgaa
     8101 cagcacagca tccacgccct ccatgctgtc ggttacgacc cggttcatca gcttgttgag
     8161 cgcgccgcca tgggcggtct ggaaacccgg cgtgtcgacc agaaccagtt gcacgtcggg
     8221 ttcgctgacg atgccggtga tgcggtggcg cgtggtctga ggacgccgcg acgtgatact
     8281 gacgttcgtg ccgaccaggc gattgagcag ggtggatttt ccgacgttcg gacgcccgac
     8341 gatggctaca tagccgctgc ggtgtacgga cgcgttcatt tggtctccgc cgcaagcgcg
     8401 taagcggacc tggctgcctc ttgttccgcg gcgcgccggt tcgcgccttc accgtaggca
     8461 tgaatgccca gttcgggaac cgcgcactcc acgcggaaat gctgttcgtg cgcctccccg
     8521 gaaaccgtca cgatcgcgta ttgcggcagc gccaggcgct ttgcttgcag gtattcttgc
     8581 aaccgcgtct tgggatcctt gccgagcgcc gtggggtcgg ttgtcgccag cagcgggtgg
     8641 tacagccgcc ccagcacctg ctcggcagac tcgaaaccgc cgtcgaggag aatggcgccg
     8701 atcagcgcct cgaaggcatc ggccagtatc gagggacggc ggaagccgcc gctcttcagt
     8761 tccccttccc cgagacgcaa atgctcgccg agcgacaggg atgcggcgat ctccgccagc
     8821 gattgctggt tgaccaggct ggcgcgcagt ctcgagagat cgccctcggg cagcttcgcg
     8881 aactgttcga agagcagccg cgcgacgcag aaattcaaca gcccgtcgcc cagaaactcg
     8941 aggcgttcgt tgtgcggcgt tccgtaactg cggtgggtaa gcgcctggcg gagcaaagac
     9001 gcatcgcgga accggtagcc cagccggcgt tcgatcgcct cgtcaggcat ggggtgacgg
     9061 gcggcgcgac agcgtcactt gctgctgctc gcctcgaaat cgatgcaggc gctgaggttg
     9121 ccgaacaact ttactttggt ctggtaaacg gcggtcagaa ccaccgaatc gccatcgcga
     9181 gtgacctcga tgtcattggc cgtgatcgcc ttgatatcgt cgatctgggc gcgcttgctg
     9241 aacagcgtct ggatttcgcg gggagaaccg cgcgcttccg gggaacgcgc aatttcacgc
     9301 aggtgctgaa ccacggttgc gtattcgatg tacgccggta ccagcttgag cgcgatgatc
     9361 gcgcccagaa cgatcagtgt agctatgaaa atcatgccga cgaaagtcac gccctgttga
     9421 cgcttcatgt gtgctcccgc tagttgatcc ggttgccgat gcgcttcagt tcaccgaagt
     9481 tccaccatat caggaaggcc ttgccgacga tgttttcttc cggcacgaac ccccagtagc
     9541 ggctgtccga actcgagtcg cgattgtctc ccatcatgaa ataatgcccg ggcggcacca
     9601 cgcagctgaa ctcgcgttca ttgtaatcgc agttctcacg ctgcgggaac tgcttgaccc
     9661 ccgccagttg cagcggcggc gcttccggat tgacgatgat gtagtgccgg tgctggtcca
     9721 gcgtttccca gaaacgcacg gcattgacga agctcaagcc accgtcgacg tactggaagt
     9781 cttgcgcagc ctccaccggc accagtttcc cgttgatgct gagccgcttg ttgcggtatt
     9841 ccacgtgatc gccgggcaga ccgaccaccc gcttgatgta atcgagcgtc gggttctccg
     9901 ggtagcggaa caccatcacc tcgccgcgtc caggctgatt gatgtcgacg atcttcttgt
     9961 tgatgaccgg cagccggatc ccgtaggtga acttgttcac gaggatgaaa tcgccgacca
    10021 gcagcgtcgg gatcatcgag cccgagggta tcttgaacgg ttccaccaga aaggagcgca
    10081 gcagaaacac cagcagaatg atcgggaaga agcttttggg gtattcgacc caccagggtt
    10141 ccctggcgtc ccggccccgg cgtttgcgca ggacgaaaat gtccagtagc cagactgcac
    10201 cggtcaccag caacagcacc agcatgagta gtgcgaaatt catcgaatgt tcaccttgtt
    10261 atccaagccg ggcgcaggct acttgctttc cacctgcagg atggcgagga acgcctcctg
    10321 cggaatttcc acggtgccga tctgcttcat gcgcttcttg cccgccttct gcttttccag
    10381 gagttttttc ttgcgcgtga tatcgccgcc gtaacacttg gcgaggacgt tcttgcgcag
    10441 cgctttcacg gtttcgcgcg cgatgatgtg cgagccgatc gcggcctgca cggcgacatc
    10501 gaacatctgc cgcggaatga gctcgcgcat gcggctggcg agttcacgcc cgcggtactg
    10561 gctgttggag cggtgcacga tcagcgacaa cgcgtccacc ttttcgccgt tgatcaggat
    10621 gtcgagcctg accaggtcgg cggcgcggaa ttccctgaac tcgtagtcca gcgaggcata
    10681 gccgcggctc accgacttga gcttgtcgaa aaaatccatg accacctcgt tgagcggcag
    10741 ttcgtaggta agcatcacct gccggcccat gtactgcatg ttcttgtgcg cgccgcgctt
    10801 gttgttgcac agggtcatca ccggcccgac atactcctgc ggcatcagga tggtggcagt
    10861 gatgatcggc tcgcggattt catcgatcct ggaaggatcg ggaagcttgg acgggttctc
    10921 gatttgcacc accgtgccgt cgcgcatcag cacctggtac accaccgtcg gcgcggtggt
    10981 gatcagcgcc atgtcgtact cgcgctccaa gcgctcctgc acgatgtcca agtgcagcag
    11041 tccgaggaat ccgcagcgga agccgaagcc cagcgcctgc gatgtctccg gctcgtaatg
    11101 cagcgacgaa tcgttcaggt gcagtttttc cagcgcgtcg cgcagcgcct cgtactggtt
    11161 ggattccacc ggatacagac cggcgaacac ctgtggcttg atttccttga aaccggccag
    11221 cggcgccgat gccggctcgt ccgccagcgt cacggtgtcg cccacctgcg ccgcctggag
    11281 ttccttgatg ccggcgatca cgaaacccac ttcgcccgcg gatagcgagt cgcgcgattg
    11341 cgatttgggc gtgaacaccc cgacctgctc gcaaaggtac gtggacttgt tggacatcaa
    11401 cagcagcctg tctttgggct tgagcgtgcc gtccaccacc cgaaccagca tcaccacgcc
    11461 gacgtaattg tcgaaccagg aatcgatgat cagcgctttc agcggcgcag ccagggttcc
    11521 gcgcggcggc gggatgcgcg cgatcacggc gtccaggatg tcttccaccc cttccccggt
    11581 cttggcgctg gcccttaccg cctccttggc ggggatgccg atgatgtcct ctatctcgga
    11641 aatcacgcga tccggatcgg cagaaggcag gtcgatcttg ttgagtaccg gcaccacttc
    11701 cacgccttgc tcgatcgcgg tatagcagtt ggcaaccgtt tgcgcttcca ccccctgcga
    11761 agcgtccacc accagaagcg cgccctcgca ggcagacagc gagcggctga cttcgtagga
    11821 gaagtccacg tgtccaggag tgtcgatcag gttgaggtgg tactgctggc cgtcgcgggc
    11881 acggtagcgc agcgccgccg tctgagcctt gatggtgatg ccgcgctccc gctccaggtc
    11941 catcgagtcg agaacctgtt cctccatctc ccggtcggac aatcccccgc aaagctggat
    12001 gatgcggtcc gcaagcgtgg atttgccatg gtcgatatgg gcaatgatcg aaaaattgcg
    12061 gatatgctgc atcagaaaaa agggcacacg acgtgccctt tacatgtggc tttcaaagcc
    12121 gcaaattgta gcggattttg cccgggaacg aggcggtttc gcggctattc cggccgatcc
    12181 gaggtcttgc tcagttgccg tccagccgca gcggcacgta caaagcgccg tcgccgcgcc
    12241 ggatcagcag cgccacgctc ttggccttgt cgtactgatt gagcaactgg gtcagttgct
    12301 cgacggattt gatgtcctgg ttgttgagcg ccatgaggac gtcgccccgg cggattccgg
    12361 ccttggccgc agccccctgc acgtcttcca ccagcagccc gccgccgata ccgagttcct
    12421 tgcgctgctc cgcggacagc tcgcttaccg tcaagccgag ccttgcgacc acgttgccgc
    12481 ccttgctgtc gcggcggccc gcgcgctgtg ccactttctc ctcgggcatc tcgcctaccg
    12541 tgacgctcac atctttggcc gcgcccttgc gccagatctg catcgtgacc ttggaaccag
    12601 gccgggtctg cgcgaccatg cgcggcaggt cggcggacga gttcaccgtt ttgccgtcga
    12661 atttgaggat cacgtcggaa ggctcaagcc ccgccttgtc cgccgagctg cctttctcca
    12721 ccgaattgac cagcgcgcca gccgccttcg gtaagccgaa cgattcggcc agctccttgg
    12781 tgacttcctg gatgactacg ccgatgcggc cgcggctgac gcgcccggcg gtccggagct
    12841 gatgggtaat gtccatcgcc acgtcgatcg ggatcgcgaa cgacaggccc atgaatccgc
    12901 cagtgcggct gtagatctgc gaattgatgc cgactacctc gcccttgaga ttgaacagtg
    12961 ggccgccgga gttgccgggg ttgatcgcca cgtcggtctg aatgaaagga acgtaatttt
    13021 cctgcggcag cgagcgaccc ttggcgctga cgatgcccgc tgtcacggta ctctcgaaac
    13081 cgaacggaga tccgatcgcc agcacccatt cgccgacctt gagacgctca ggatcgccga
    13141 tcttcaccgc cggcaatccg cttgcctcga tcttgatcac cgcgacgtcg gtgcgcttgt
    13201 ccgagccgat gaccttggcc ttgaactcgc gcttgtcgtt gagcttgacc gtgatgtcct
    13261 cggcggcttc aaccacatgg gcattggtga ggatgtaacc gtcggcgctg atgatgaagc
    13321 ccgacccgag cgaacgtgct tcgtaatcgc gcggctgtgg cggtccgaag cggcgaaaaa
    13381 agtcgtagaa cgggtcatcc tccggcaccg gcgactgcgg gccgctgcgc gccgccgagg
    13441 tcgtgctgac gttcaccacc gccgggccct gcttttccac gagctcggtg aaatccggca
    13501 gtccctggac ctgtgcgacg cccgcgagcg gcacgaacag cagcaggcag aaagcgagga
    13561 gggcttttct tgacatcgga aatttcctct gtgggcaaaa ctgagttgaa gaaggcgacg
    13621 agaacggcgt tacttgccgc ggaaggacac ggaattagcg atctgcgtga cagtggatgc
    13681 gggggcttcg ccgagcacgg tgacgacctg gtcgtcgatg gtgcgtgtgt agatgttgat
    13741 ggcaccctga tgcgacaggc cctcgttgat ccgggcccgg tttgccatcg gctcgatgaa
    13801 caccgacacc gccgccagtc cgtccgaata caccatgtgc gtcaccggaa tcgaattgcc
    13861 gagcttgctg cggcgcattt ccatgatctt gcggaagccg gccgggaaat tcctcaccgc
    13921 ccagccggac tcgacattgc tggtcaggtt gtgggcgaaa cgatccaccc gccattcggg
    13981 gaagctcacg ctgaacgacg gcttgaccat gtcgcggctg atcccgctgc cgatggcgag
    14041 ctgggtaaag ctgaattgct ccaccaggtt gtggcgttcg ttgaccatgc gcgccttgag
    14101 caacagcccg gaattcgtct ccgcccagaa cttgtggcca tagcgcatgc cgtcgcgcgg
    14161 ttccagcacc agcgcctgcg aatcgaagcc ggctatgcgc tccggctggc ccatgcggat
    14221 ctgatagtac tcggtgagcg ccgagagctg atccggcagc agcgccggga aggtgcgccg
    14281 cgccacgcgc ttctcgctgc gcatgacctt ggcgtcgagg taatagcaca tgacttcatc
    14341 gttgttgcgg atgatctcgc ggcgcgggcc gtccagggtt tcgagtttct cgtattcacc
    14401 gctggcgtcc gcaaaatgca cgatgcgcga ggtttcgacc tggtcagagt gctggtaaac
    14461 gaaggtgccc gcgtagttaa gctcgcgtgc cgcgtcggcg atcttctgca acatcgccag
    14521 cgcttcagtt tcctgtaacg ggtcggccag cgcgaccccg gcgctaaatc caagcccgaa
    14581 cagcgcggga agcaggagcg ccgagcgagc cgtcatcaac gttgttccgg attttgcccg
    14641 gatacggtcc tgacgtaaga agccacaccc tgcatcgcgg tgctcgggga aaactgctga
    14701 tgcgcgagca ggtagtcgtt gacggcatcg ttggccggcg tggcagccaa ctgggtttgg
    14761 actcccggtg gcgcttgatt gaccgcgact ttctgctgcg gagcgaaggg actgtcgaac
    14821 agtgcgagcc atgtgaccag cgccaccccg ccgagacacg cggccgccga aagcgctacg
    14881 cggcgtacgt ttcgcaactg tattcgcggc ctgggcgcca atatcgtagg ctcggcggcg
    14941 agcctggcgc tgatcttgac caccagaccg gatgagtacc ccgcttcacc tcgcatggca
    15001 tctccgatca gatggtaggt ctcccaagcg gttcgtagtt ccggatcctc cttcagccgc
    15061 ctcacctgac tggcggcctc gtggtcaccg agttcgccat ccatgaatgc agagattcgc
    15121 tccatactta ccacctttta tccttgctcg tctccagcaa cgggcgcagc ctgtcggcga
    15181 tggtttcgcg ggcccggaaa atccgtgagc ggaccgtgcc gatggggcag ttcatcacgt
    15241 ttgcgatgtc ttcgtaactc attccctcga tctcgcgcag cgtaatcgcg gtgcgcaact
    15301 cttcaggcaa ctcttccatc gcctggttca cggtggccgc tatctgcctg gtcatcagtt
    15361 ccgcttcggg ggtattcagg tcccggagct ggtcgccatc ctcaaagttc tcagcctcct
    15421 cgctgtcgaa ctcggtagtg gtgggggcgc ggcgccccat cgccaccaga taattcttcg
    15481 cggtgttgat cccgatccgg tacagccagg tgtaaaacgc gctgtcgccc cggaatgagg
    15541 gcaacgcccg gtaggccttg atgaaagcct cctgggccac gtcttccact tcggcagggt
    15601 cccgaatgaa acgggacagc aggcgcccga gcttgcgctg atacttcgct accaacagct
    15661 cgaaagcgtg cttgtcgccg cgttgagccc gctcgaccaa ctgctggtcg atttcacggt
    15721 cgcccatcta gccacatttc ctgaatattc tggttgttgg ttgcactggc gcctcgaatg
    15781 ggcgcctaag tatacccgcc aagacttgtc caggaaacgc accgcgattt gctaagccag
    15841 agacagcgat tgtccggcat tagttcacct gcggacgcgg ttatttgccc ggggccgatc
    15901 gcgcgccggc ggttaggtta cactgccgcg ctccgaacca gcgagaaaca catgaggcgt
    15961 ttcgatgtcg cgatcgtcgg cagcggcctg gccggcctta cggtcgcgct caatctggcc
    16021 gaccgcaaga aagtcggcat cgtcaccaag cgcgaattcc tggatggcgc ctccaactgg
    16081 gcccagggtg gcattgccgc ggtgcttgcc aaggacgact cgcccgagga tcacatccgc
    16141 gacacggaaa tcgccggcgc gggactgtgc aacgccgagg tcacccgcta cgtggtcgag
    16201 cgcggacgcc aggccatcga atggctgatc gggcaaggtg ttcccttcac ccgggatgaa
    16261 accagcgacg tcggttatca cctcacccgc gagggtggcc acagccaccg ccgagtaatc
    16321 cacgcggccg acgctaccgg acacgcggtc cagaccacgc tggaagccaa ggtcaaggcg
    16381 catccgaaca tcacggtgct ggaccattac atggccgtgg atctgatcac cgggcccaag
    16441 ctcggacagc ccgagcggcg ttgccacggg ctctacgcgc tcgacgagaa cgccggacgg
    16501 gtggtcacga tcggcgccga acaggtggtg ctcgc
//
LOCUS       DASQOP010000121        16787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_51347, whole genome shotgun sequence.
ACCESSION   DASQOP010000121 DASQOP010000000
VERSION     DASQOP010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 16787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16787
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51347"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1084)
                     /locus_tag="VE030_04805"
     CDS             complement(<1..1084)
                     /locus_tag="VE030_04805"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007731040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HZF22771.1"
                     /translation="MAAVAETLAGATRDGGGTVPLFELRDISKSFPGVQALDGVSLEI
                     RAGEVHVLLGENGAGKSSLMKVLCGAYQADKGEFLYRGERVAIRSPSDARALGIAVIF
                     QEFSLVPYLNVAQNIFLGREPPGRIPGGVDHGRMHARARELLHLLGLEVDTRALIHTL
                     GVAQQQLVEIAKALSQEARILVLDEPTSALSGRESERLFAMIRRLQSQGVAMIYISHR
                     LAEVFQIGDRITVLRDGRKVGSLKPKDTTPDALVGLMVGRKVDTTYARQYCESPGEVV
                     LEVRGMAAANGVNGIDLVVRAGEIVGLSGLVGAGRTEVARALFGADRVTAGEVRILGR
                     SRIGGPDRAVRLGMALVPEKRKQEGLA"
     gene            complement(1112..2113)
                     /locus_tag="VE030_04810"
     CDS             complement(1112..2113)
                     /locus_tag="VE030_04810"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HZF22772.1"
                     /translation="MKLGVAGTGGMDFNNPTGTRGLVAVDKVGGKVRLYEPGTYREMA
                     AIEVERLPHEVAISPDHQIAYVSIYGAGTFGNNPNPAQTIAVIDLDRREQVDAISVAP
                     YFAPHGMMVAATGKLWVSCDASAKLIVVDPETRAIEEAFDTGSFGSHWIALTPDGAKI
                     YISNKTDSVGVFDVATRALAATIALPSEKQGTEGIAASPDGSRVLVVDNFDTRVHVID
                     AVTDAVIDSVALAGNPPTNPKRSRNVRVRYSLDGRYVLTSNYASGVIYIHDADDLRRQ
                     TPLIVAKGPMGFAFAPDGNAAIVANHDSGIATVIDLAERRITGYFDGGSGIETLAFY"
     gene            2422..3726
                     /locus_tag="VE030_04815"
     CDS             2422..3726
                     /locus_tag="VE030_04815"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HZF22773.1"
                     /translation="MAKKKTREPRVVHPDDIDPHHNWSRPIQAPGHTQVDFEERVNFR
                     RLHDYRLSRVRAALAGSGLGALLSFDQHNIRYTTSTVIGEWARDKLTRYSLLTGTGEP
                     YIWDFGSAAKHHRLYAPWLHQDHCRAGLLGMRGAVGTDLVLFRDAAREVKSILDAEGV
                     GDMPLGLDVVEPPMLFELQKLGIEVRDGQQVMLQAREVKSADELTLLNMAAAMVDGVY
                     QDIAEALKPGVRESQIVALATGRLYEMGSDCVEAINSISGERCSPHPHNFTDRLIRPG
                     DQAFFDVIQSFMGYRTCYYRTFSVGRATPAQRTAYRKAREWMDRAIDLLRPGVTSDKI
                     ARAFPKAQEIGFASEMDAFGLNFCHGLGLGLHERPVVSRLNSFKEPIELKVGMVFAVE
                     TYCPAKDGVSAARIEEEVILTPTGPQIISLYPAQELPVANPY"
     gene            3710..4639
                     /locus_tag="VE030_04820"
     CDS             3710..4639
                     /locus_tag="VE030_04820"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZF22774.1"
                     /translation="MQTPIDAKVGWIGTGRMGFKMAQRLARAGCDLTVWNRTRAKAEP
                     LARDGARIANLLADLGGCDVVFCMLSTWDDVKQVMAGTGGLLSHDIRMPLTVIECSSI
                     SLQGSAELRTILAARGIELLAAPVSGNAKVVAAGKLSFVVSGPKAAYQRARPLFDLIG
                     QSSTWVGEGELARIVKICHNVFLGVIAQSLAEVTVLAQKAGVERHAFLDFLNKSVMGS
                     MFTRYKTPAFVNLDFKVTFTPELLRKDMDLGLDLSRRLAVPMPLAAVSREQIQSLIGH
                     GFAEQDFAALLVLQARASGIELEPEHAAVDDGL"
     gene            complement(4658..5419)
                     /locus_tag="VE030_04825"
     CDS             complement(4658..5419)
                     /locus_tag="VE030_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22775.1"
                     /translation="MNRIYLSLLAVFLVSAGISERALSQDTKALANAVVTAADRSPED
                     RALDAGRKPVELLAFLGVRPGWRIADLGAGGGYTTELLQRAVGSEGVVYGQNSKKLLD
                     MFLEKPWSARLAKPAMNGVVRLDRDFDDPFPDGLRPLDAVTMVLFYHDTVWLGVNRAA
                     MNKAVFAALKPGGVFVIVDHSARPGDGIDVADTFHRIEENVVKSEVQAAGFVLDGQAD
                     FLRNKDDTRDWNDSPRAAGERRGTSDRFVLRFRKP"
     gene            5804..6295
                     /gene="msrB"
                     /locus_tag="VE030_04830"
     CDS             5804..6295
                     /gene="msrB"
                     /locus_tag="VE030_04830"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HZF22776.1"
                     /translation="MNRRQLLASIPALLVGLPALAKENRVDPLVKPKSEWKTLLPAMS
                     YRVLFEEDTERAGTSPLNKEKRAGTYVCAACYLPIFSSETKYDSGTGWPSFFDYLPGA
                     LGTKKDTRLIWPRTEYHCVRCGGHQGHVFDDGPKPIGLRYCNNGVALRFVPKEEKLPE
                     LRT"
     gene            complement(6417..7457)
                     /locus_tag="VE030_04835"
     CDS             complement(6417..7457)
                     /locus_tag="VE030_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22777.1"
                     /translation="MNARSGILPPLAASPWFAAAPAAAALVYPLLLAGLHRGGRMLQG
                     DVVAGSAVIVVSLLLVLAVPAFGFYTAVRLGRIAQPTIAEARARGFAHLAYACPPLFT
                     LIGVLLYVNSSSISDLAVWAAIWLAAGAAILVSASRDEAAPRRHTSTGLRFAHGASAA
                     AILAIFLVLHIGNHLVGLWGIEAHKAVMKILRHWYRNPVVEPALVLLIVFQILSGAWL
                     LRAKLAQRAGFFETLQSATGAYLGIFLTSHVTAVLVLGRSFVGIDTDFYWASGEAAGL
                     ITDAWNVRLIPHYLLAVWGIITHAGCGLRTVMLSHHVRDTNADKVAGWVSACGALVAI
                     AIVSAQSGLRLG"
     gene            7659..8306
                     /gene="can"
                     /locus_tag="VE030_04840"
     CDS             7659..8306
                     /gene="can"
                     /locus_tag="VE030_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HZF22778.1"
                     /translation="MNDLSHLLKNNRAWAESVRRREPDFFDQLAQLQTPQYLWIGCSD
                     SRVPANQITGLAPGEVFVHRNVANVVVHADLNCLSVLQYAVDVLKVKHVIVCGHYGCG
                     GVIAAHDHVKLGLIDNWLRHVQDVGQKHRDLLAREKAREKRVARLCELNVIEQVVNVA
                     QTTVVQDAWARAQALAVHGWIYGIRDGLIRDLGMSVSKAEDLQSRYASAVGAAER"
     gene            8564..8941
                     /locus_tag="VE030_04845"
     CDS             8564..8941
                     /locus_tag="VE030_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22779.1"
                     /translation="MSIVLPLIILALALSPSGEAAGAKDEIRAAETSTAEFAVYALSR
                     GKGVPEPTRGVFRKARTLLEGAKRRGEVIRVKETRIGLEGETRLCVEAKDAAAARALL
                     RELRAIAENVELFNVVVEPCLKK"
     gene            8992..10050
                     /locus_tag="VE030_04850"
     CDS             8992..10050
                     /locus_tag="VE030_04850"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HZF22780.1"
                     /translation="MKYSLAALPGACVLACSFLLLTGCEPATGAGNSADTECAMPTTK
                     DELQPRASEGHAGTQIRPATERVEATPETVAQAMAQMERRAKEECRQWADKAFEEFER
                     STYREPFPGGKYIVSGDIAISDRQRLREFFETRIKGESSETVPGALIVHRVGGRDAIW
                     DSPLRKHLTYCVSSAFGDRRQQVIRDMEAASGAWEAVADVDFIHVTAEDADCTPANGN
                     VVFDIRPVNVDGQYLARAFFPNEERSERNLLIDESSFRLDPHGALQLVGILRHEIGHT
                     LGWRHEHTRPESGTCFEDNNWRPLTDYDRFSVMHYPQCNGGADWSLKLTHMDKNGAAC
                     VYGAAPGFNVDPQICPRG"
     gene            complement(10129..10443)
                     /locus_tag="VE030_04855"
     CDS             complement(10129..10443)
                     /locus_tag="VE030_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22781.1"
                     /translation="MPTLRFDERSGSPGTVVVSESDHELGTIRFESPSGRYKFTPTTP
                     KLWPIRCLVDADPKELNARIEALVCALMEISASIAAKAASRHAAHGGLTSGQETQDPP
                     GE"
     gene            complement(10589..10984)
                     /locus_tag="VE030_04860"
     CDS             complement(10589..10984)
                     /locus_tag="VE030_04860"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HZF22782.1"
                     /translation="MKFFFAAVALIAVPAHAENWKEIDRDATATYYVDADSLSRNEEI
                     VSLIKKAVYRVSLWPVQLSDNATVKETAGVIEENCRNRHHRVLSMDMFNLFGERIWSS
                     GKMRRIWESVEPNSNGERTHEFACAWTPG"
     gene            11476..12501
                     /gene="dctP"
                     /locus_tag="VE030_04865"
     CDS             11476..12501
                     /gene="dctP"
                     /locus_tag="VE030_04865"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HZF22783.1"
                     /translation="MIHSLKVVARSATLAVLAGLTLASAAGTAEAQHKWKVQNLYGPN
                     TTVYKDFQAFAARVKEATKGEVEIQPFPVATIVPQAEALDAIKAGLLDGAVGTMAYQG
                     GKEAAAAFWDMTAAYDYPLHLLMWYRQGGGMEVMNKISAKWDAVFLGPVILGLESIPS
                     KKPIRSIADFKGVKMRAPDGIPAEIFALLGASVSVMPGTEVYTALDTGKIEATDWGTL
                     SVNDEAGYNRIAPYAIYPGIHSMNSVDFVVSKRKWNALSAEQKAIVTAAVDEWCLRTW
                     MDQELEDRKAAAKRDPGTLIQWGEKEKGEFRQVSRKVWEKWSKKSPLAKEIYESQTKF
                     LKSIGKL"
     gene            12539..13111
                     /locus_tag="VE030_04870"
     CDS             12539..13111
                     /locus_tag="VE030_04870"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HZF22784.1"
                     /translation="MSRARFVPADNKKKGGIMAESAPDAEATGIGAVDRAIEAIAKAL
                     SFTFALSIVVTIYDVVADVVFKSPTVWVYDVVTTAIAVAFLIGGSYSLMRREHIRITA
                     VHDRLSRRTRLRCDIATSILASVYLVAFAWFSFNMAWLAVQDWEVGGSAWAQPTPVIV
                     KVSMVIGALLMIVQMISNIRGDLKALRNEA"
     gene            13115..14431
                     /locus_tag="VE030_04875"
     CDS             13115..14431
                     /locus_tag="VE030_04875"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HZF22785.1"
                     /translation="MGIETISLLMLAAFVLLIIVLGMPIAFATGLIAVVFCALFADLD
                     SLAIVTFRTYGFVNSYVLLSVPMFLMMANILDKSGVAHDLYEALKIWAGRLPGGVAVM
                     TLIAASVMAAMTGIIGGEVILLGLVALPQMLRLRYDSKLAIGVVCAGGSLGTMIPPSL
                     VLVFYGLTTSTSIGDLFLASTIPGIVLALIYIIYVLIRCGLNPKLGPPAPPELRNIPL
                     RAKFAALRSVILPLLIIFSVLGSIYTGITSVSEAAAMGVAGVTVAAAIRRTLTLAMLR
                     DSLYSAMSTCGLLIWLSIGANAMVGVYNLLGGITYLKSIMTGLPLAPIGVILIMMAIF
                     LVLGMFMDWFSIMLLTMPIFAPTVTALGYDLIWFGVLFNITMQVGYLSPPFGQAAFYL
                     KSVAPPEISLNHIFASLFPFIGLQIIGLIIVLFIPEVAMFLPRLLH"
     gene            complement(14489..16648)
                     /locus_tag="VE030_04880"
     CDS             complement(14489..16648)
                     /locus_tag="VE030_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZF22786.1"
                     /translation="MTTGALRRGIAATAIALLLAVCATGTGLCDDSTKIRIADNDLGG
                     VVKSTNGPEAGVWVIAETSDLPTRFAKIVVTDDQGRYLMPDLPKASYSVWVRGYGLVD
                     SPKVQATPGKRLDLSAVVAPSAAAAAEYYPAIYWYSLLKIPAKSEFPGTGIDGNGIDP
                     GMKTQTHWLNTIKTNGCMSCHALGTVGTRTMPKEFADMNSTEAWGRRIASGQAMNNMV
                     TIAGRLGTGRALAQWADWTDRIAAGELPFAKPERPQGIERNLVLTLWDWSRPHAYMHD
                     LIGTDKRMPTVNTNGKFYGAPENSTDYWPVLEPLKHTATEVKSPVRDPNTPSHRTDPM
                     APSPYWGPDPIWDSQTSTHNQMMDEKGRVWAAARIRHPNNPDACRKGSDHPSAKVFPV
                     ESASRHLSMYDPATGKWTLISTCFPTHHLIFAEDANNTLWASAGVSGPGVVGWLNRKM
                     FEETGDEMKSQGWTPFILDTNRNGRRDEWVEPDQPLDPAKDKRVAVNLYSVVVNPVDG
                     SVWGTSLAPFPSYAVRVAPGSDPTHTAITEVYEPPLPAYGARGGDIDRNGVFWASFAS
                     GHLGSFDRRKCKAPLNGPNATGKHCPEGWTLYRFPGPQFRDVQDDGSAEASYYVWVDW
                     FNTLGLGNNVPIATGNTSDSYFALVDGKFVTLRVPYPMGFFAKWGEGRIDDPKAGWKG
                     RALWATDSTRAPFHSEGGTQRTAKVYKIQLRPDPLAR"
BASE COUNT         3144 a         5145 c         5328 g         3170 t
ORIGIN      
        1 gtgcaagtcc ctcctgcttg cgtttttctg gcacgagcgc catgcccagg cgcacggcgc
       61 gatccggccc gccgatccgg ctccggccca ggatccgcac ctctccagcg gtcacccggt
      121 cggcgccgaa gagtgcgcgt gcgacctccg tccggcctgc gcccacgaga ccgctcagac
      181 cgacgatctc gcccgcgcgc acgaccaagt caatgccgtt cacgccattg gcggccgcca
      241 tgcccctgac ctcaagtacg acctcaccgg gggactcgca gtattggcgc gcataggtcg
      301 tgtccacctt gcgcccgacc atcaggccca ccagcgcgtc cggcgttgtg tccttgggct
      361 tcaagctccc gactttgcgc ccgtcgcgca aaacggtgat ccgatcgccg atttggaaca
      421 cctcggcaag acggtgagag atatagatca tcgccacgcc ctgcgattgc aaacgccgga
      481 tcatggcgaa gaggcgctcg ctttcgcgcc cggagagcgc cgaggtcggc tcgtcgagta
      541 cgaggatccg cgcctcctgc gagagcgcct tggcgatttc gaccaactgc tgctgcgcta
      601 caccgagcgt atggatcagc gcacgcgtgt cgacctccag gcccagtaga tgcagaagtt
      661 cacgcgcccg tgcgtgcatc ctcccgtggt caacgccgcc tggaattcgt ccgggcggct
      721 cacgtccaag aaagatgttc tgcgcgacgt tcaggtaagg cacgagggag aattcctgga
      781 agatgaccgc gatgccgagc gcgcgcgcat ccgacgggga acgaatcgcg acccgctcgc
      841 cccgatagag aaactctccc ttgtcggcct ggtaggcgcc gcacagcact ttcatgagcg
      901 aggacttccc ggcgccgttt tcacccagta acacgtgaac ttcgccggcg cggatctcga
      961 gcgacacacc gtcgagcgcc tggaccccgg ggaacgattt gctgatgtcg cgcagctcga
     1021 ataggggcac cgtgcccccg ccgtcccgtg tcgctccggc aagcgtttcc gcaacagccg
     1081 ccactacgcc tccctctctc gcgtgctgcg ctcagtagaa cgccagcgtc tcgatcccgc
     1141 tgccgccgtc gaaatacccc gtgatccggc gctccgcaag gtcaatgacc gtggcgatgc
     1201 cgctatcgtg attcgccacg atcgccgcgt tcccgtcggg tgcaaatgcg aaccccattg
     1261 gccctttggc gacgatcagc ggcgtctgcc ggcgcagatc gtcggcgtcg tggatgtaga
     1321 tcacgccaga ggcatagttc gacgtcagaa cgtagcggcc gtcgagcgaa taccggaccc
     1381 gcacgttgcg gctgcgcttc ggattggtcg gcgggttccc ggcgagcgcg acggagtcga
     1441 tgaccgcgtc ggtcaccgcg tcaatgacat gcacgcgcgt atcaaagttg tcgacgacca
     1501 gcacgcggct gccgtccggc gatgcagcga tgccttcggt gccctgtttt tcgcttggca
     1561 gcgcgatggt cgcggcgagc gcgcgcgttg cgacgtcgaa cacgccgacc gaatcggtct
     1621 tgttggagat gtaaatcttg gccccatccg gtgtcagcgc gatccagtgg ctgccgaacg
     1681 agccggtgtc gaacgcctcc tcgatcgcgc gcgtttcggg atcgacgacg atcagcttgg
     1741 cggaggcgtc gcacgacacc cagagcttcc cggttgcggc caccatcatc ccatgcggcg
     1801 cgaaatacgg cgccacgctg atcgcgtcaa cctgctcgcg ccggtcgagg tcgatgaccg
     1861 cgatggtctg cgccgggttc ggattgttgc caaaggtgcc ggcgccgtag atgctgacgt
     1921 acgcgatctg gtggtcgggc gaaatggcga cttcatgcgg cagcctctcg acctcgatcg
     1981 cggccatctc tcggtacgtg ccgggctcgt agaggcgcac cttgccgccg accttgtcca
     2041 cggcgacgag accgcgcgtg ccggttggat tgttgaagtc catgccgccg gtgccggcga
     2101 cgccgagctt catgcctccc cctttagtca cgcactcgag aggtgcggac gagagcttct
     2161 acatatacac gcggggaaag cgaagcaccc gctctaccgc gtcaggcgaa ctgcagcttg
     2221 ttcggacacg aagcggctgt caagtgagcg tgtctgaagt ccatacggtc cggctcccgc
     2281 gcgatcgcga cgatggcgga tcgggtgtaa tctacagtgg cagacgcgtc cgaggctcgg
     2341 cgcacctggc cgatttgcga gaaagtgttc aaggagtgaa gagcgaaatc aggacacggc
     2401 aaaacaggag cagtgaccac aatggcgaag aagaaaacac gggagccgag agtcgttcac
     2461 cccgacgaca ttgatcccca tcacaactgg agcagaccga tccaggcccc ggggcacacg
     2521 caggttgatt ttgaggagcg cgtcaatttt cgccggctgc acgattaccg cctgtcgcgg
     2581 gtgcgtgcgg cgctggctgg ttccgggctc ggcgcgctgc tgtcgttcga ccagcacaac
     2641 atccgctaca cgaccagcac cgtgatcggc gagtgggccc gcgacaagct cacgcgctac
     2701 tctcttctga ccggcaccgg cgagccttac atctgggatt tcgggtcggc ggcgaaacac
     2761 catcgcctgt acgcgccctg gctgcaccag gaccactgcc gtgccggact gctcggcatg
     2821 cgcggcgcgg tgggcacgga cctcgtcctg ttccgtgatg ccgcgaggga ggtcaaatcc
     2881 atcctcgacg cagaaggcgt aggcgatatg ccgctgggcc tggatgtcgt tgagcccccc
     2941 atgctattcg agttgcagaa gctgggaatt gaagtgcgcg acggtcagca ggtgatgtta
     3001 caggcgcgcg aggtgaagag cgcagatgaa ttgacgctgc tcaacatggc cgcggcgatg
     3061 gtggacggcg tataccagga catcgcagag gcgctcaaac cgggcgttcg ggagagtcag
     3121 atcgttgcgc tcgccaccgg ccgtctgtat gaaatgggat cggactgcgt cgaggcgatc
     3181 aactcgatct cgggcgaacg ctgctcgccc catccgcaca atttcacgga ccggctgatc
     3241 cggccgggtg accaagcgtt cttcgatgtc atccagtcgt tcatggggta tcgcacctgc
     3301 tactaccgaa ccttcagcgt cggacgcgcc accccggcgc aacgtaccgc ctaccgcaaa
     3361 gcgcgcgagt ggatggacag agccatcgac ctgttgcgtc ccggcgtgac gagcgacaag
     3421 atcgccagag cgtttcccaa ggcgcaggag atcggctttg ccagcgagat ggacgcattc
     3481 ggacttaact tctgtcatgg cctgggcctc gggctgcatg agcgccccgt agtgagccgg
     3541 ctcaactcct tcaaggagcc cattgagctc aaggtgggga tggtgtttgc cgtggagact
     3601 tactgcccgg caaaagacgg cgtgtctgcc gcccgtatcg aggaggaagt gatccttact
     3661 ccaacgggtc cgcagatcat ctcgctttat cccgcacagg agctgcccgt tgcaaacccc
     3721 tattgatgcc aaggtaggct ggatcggcac cgggcgcatg ggattcaaaa tggcgcagcg
     3781 cctggcgcgc gcgggctgcg acttgacggt gtggaaccgc acccgcgcca aggcggagcc
     3841 gcttgcgcgg gacggtgcca ggattgcgaa tttacttgcg gatctcggcg gctgcgacgt
     3901 ggtgttctgc atgctgtcca cctgggacga cgtgaagcag gtgatggcag gaaccggtgg
     3961 gttgctatcg cacgacatcc gaatgccgct aacagtgatc gagtgctcct cgatctcctt
     4021 gcagggctcg gcagaactgc gcaccatcct tgcggcacgc ggcatcgagc tgctcgcggc
     4081 gccagtcagc ggcaacgcga aagtcgtggc agcaggcaag ctttccttcg tagtgtccgg
     4141 gcccaaggcg gcctaccagc gggctcggcc gctcttcgat ctaatcggcc aaagctccac
     4201 ttgggtgggt gaaggcgagc tcgcgcgcat cgtcaagatt tgccacaacg tcttcctcgg
     4261 cgtgattgca caatcgctcg cagaggttac cgtgctggct caaaaggcgg gggtggagcg
     4321 gcatgcattt ctggattttc tcaacaagag cgtgatgggc tcgatgttca cgcgctacaa
     4381 gacgcccgcc ttcgtcaatc tcgacttcaa ggtcacgttc acgcccgagc tgctgcgtaa
     4441 ggatatggac ctcgggctcg acctaagccg gaggttggcc gtgccgatgc cgctggcggc
     4501 tgtcagcaga gagcagatcc agtctctcat cggacatgga tttgcggagc aagacttcgc
     4561 cgctctattg gtactgcagg cgcgcgcatc gggaattgaa ctcgagccgg aacatgcagc
     4621 agtagacgac ggactttgat aatttttggg tcaatggtca gggcttccgg aagcgcagta
     4681 cgaaccgatc gctggtgccg cggcgctctc cggcggcacg cggcgaatcg ttccaatccc
     4741 gggtgtcgtc cttgttgcgc aggaaatcgg cttgcccatc gagtacgaac cccgccgcct
     4801 gaacttccga cttgacgaca ttctcctcga tccgatgaaa cgtatcggcc acatcgatac
     4861 cgtctccggg tcgcgcgctg tgatcgacaa tcacaaatac gccacccggc ttgagtgcgg
     4921 cgaacacagc cttgttcatg gccgcgcgat tcaccccgag ccagacggtg tcgtgataga
     4981 acagcaccat cgtgaccgcg tcgagcggac gcaagccgtc tggaaaggga tcgtcgaaat
     5041 cgcgatccag acgcacgacg ccgttcattg ccggcttggc gagccgcgcg ctccagggct
     5101 tttccagaaa catgtccagc aatttcttgg aattttgccc gtagacgacg ccttcgctgc
     5161 ctaccgcccg ctggagtaac tcggttgtat agccgccgcc tgcacccagg tcggcgatac
     5221 gccaacccgg ccgtacgccc agaaacgcga gcagctcgac cggtttgcga ccggcgtcca
     5281 gcgcacgatc ctcagggctg cggtctgccg ccgtgacgac agcgtttgca agcgccttcg
     5341 tatcctgact caacgcgcgt tcagagatgc cggcggatac caggaacacg gccagcaacg
     5401 acaggtagat tctattcatg catttctcca atcagtgaag atccattttg aatcgccaga
     5461 acgagggtat ttctttcgga cagagatggt agcgcaaaaa tatgtcgctc gttatcgcca
     5521 aactccccct ccggttttca cgccccggga gctccgcttt atgacctccg cgatcccggc
     5581 agacgtgtag tgccgcagtg ctctccgcat ttcgtatgaa cggatttggc acccagttgt
     5641 ctgtttcggc atccaatttg ttgcatccac catggtgcgc ggggacgtat atttggccgg
     5701 aacgtgcctc tgtgtcccga tcgtcgtcga ggcgtccggc tgtacggtac tgacgcatcg
     5761 acaggtcaac atgtagtcga tacagcattt ataaggacat cccatgaata gacgccaatt
     5821 acttgcgagc attcccgccc tccttgtcgg gctaccggcc ctggcgaagg aaaaccgtgt
     5881 ggatcccctg gtgaaaccta aatccgaatg gaagaccctg cttcccgcca tgagttacag
     5941 ggttcttttc gaagaagaca ccgagcgcgc gggaaccagc ccgttgaaca aggagaagcg
     6001 cgcaggcact tatgtgtgcg ccgcgtgcta tctgccgatc ttcagcagcg agacgaagta
     6061 tgacagcggc acggggtggc cgagtttctt tgattatttg ccgggcgcgc tcggcacaaa
     6121 aaaggatacg agactcattt ggccgcgcac cgaatatcac tgcgtgcgct gcggcgggca
     6181 tcaaggccat gtcttcgacg acggtcccaa acccatcggt ttgcgctact gcaacaacgg
     6241 cgtggcgctg cgcttcgtgc cgaaggaaga aaagctgccg gaactgcgaa cctgaaacgg
     6301 tggtgccggg ctgaatcgta cagcgcgacc atgcagtagg ccgtcagggc ccggattcca
     6361 aatggattgg aaccggactc cgtcgttgcg agagcctcta tggcgaaccg ctggactcaa
     6421 cccagacgca gcccggactg ggccgaaacg atcgcgatcg cgacgagcgc gccgcacgcg
     6481 ctgacccacc cggcaacttt gtctgcgttc gtgtcgcgca cgtggtggga gagcatcacc
     6541 gtgcgcaagc cgcaacccgc gtgggtgatg attccccata ccgcaagcaa gtagtgcggg
     6601 atgagccgta cgttccatgc gtcggtgatc aatccggccg cttccccgga ggcccagtag
     6661 aaatcggtgt cgataccgac aaaactgcga ccgagcacca gcacagcggt gacatgcgac
     6721 gtgaggaaga taccgagata agcgcccgta gcgctctgta gggtctcgaa gaagccggcg
     6781 cgctgcgcaa gctttgcgcg cagcagccac gcgccgctca gaatctgaaa cacgatcaaa
     6841 agaaccagcg ccggctcgac taccggattg cggtaccagt ggcgcaggat cttcatcact
     6901 gccttgtgcg cttcgatccc ccacagccca acgaggtgat taccgatgtg cagcacgaga
     6961 aagattgcca gaattgccgc cgccgatgcg ccgtgcgcaa agcgcagccc tgtcgacgtg
     7021 tgccgtcggg gtgcagcttc gtcccgcgat gcggacacga gaatcgcggc gcccgcggcc
     7081 aaccagatag cggcccagac ggccaggtcg gaaatcgaag aggaattgac gtagagcaga
     7141 accccgatca gcgtaaacaa cggcgggcag gcgtaggcca ggtgcgcgaa cccccgtgca
     7201 cgcgcttccg ctatcgtggg ctgcgcgatc cgcccgagac ggaccgcggt ataaaaccca
     7261 aacgccggaa ccgccagcac cagcaggagc gagaccacga taacagccga tccggccacg
     7321 acatcgccct gcagcattct gccgcctcga tgcagacctg ccagcagcaa tggatagaca
     7381 agcgccgccg ctgccggtgc agccgcaaac cacggacttg ccgcgagcgg cggcaggata
     7441 ccgcttcgag cgttcaccgc gtattcctcc aacttgaggc atcattcccg gaaaggatcc
     7501 gggatcgcct tgctgcggaa gagaaacctg gggttaattt accaccatcc ttgcatctcg
     7561 agcggtgcga gaaaccggcg gagttctacg ccttataatc tagcgtcaca aagtctgaag
     7621 ccgaacaaga tcgtggcgat tcctcccggg gctcacgcat gaacgacctg agtcatcttc
     7681 tcaagaacaa tcgcgcctgg gcggaaagcg tgcgccggcg cgaaccggat ttcttcgacc
     7741 agctcgcaca gctgcaaacg ccgcagtacc tttggatcgg ctgctccgac agccgcgttc
     7801 ccgcgaacca gatcaccggt cttgcgcccg gcgaggtgtt cgtccatcgc aacgtcgcca
     7861 acgtggtggt acacgccgac ctgaattgcc tgtcggtgct gcagtacgcg gtggacgtgc
     7921 tgaaagtcaa gcacgtcatt gtttgcggcc attacggctg cggcggcgtg atcgccgcgc
     7981 acgaccatgt caagctgggg ctgatcgaca actggctgcg ccacgtgcag gacgtcggcc
     8041 agaagcaccg cgacttgctc gctagagaaa aggcaaggga aaaacgtgtc gcccgcctgt
     8101 gcgagcttaa cgtgatcgaa caggtggtga acgtcgccca gactacggta gtgcaggacg
     8161 cctgggcccg cgcccaggcg ctcgccgtgc atggctggat ctacggcatc cgggatggat
     8221 tgatacgcga ccttggaatg agcgtctcga aggcagaaga tctgcaatcg cgctatgcct
     8281 cagcggtcgg cgcggcagaa cgctagccgc ttcggctgga atagcagtca accgtcgggg
     8341 aaacagcaga tcgcgggtga gcgaggccgt gaggacctga cgtagcgcga agggaccacg
     8401 ggacgcggcg aactttgtgt cgagaaagcc tgacaaagag agacagatcc tgacggcgtt
     8461 ttacaggccg ccgggccggg agtggacgat gcgggaagag gatgcgaggg tcccatgttt
     8521 cgcggcacga gccaagaacg ccgcgaacgg ttgtctgcgt cccttgtcga tcgttctgcc
     8581 tctgatcatc cttgccctgg cactgtcccc gtctggtgag gctgccgggg caaaggacga
     8641 aatacgggcg gcggagacat cgaccgccga gttcgcagtc tatgcgctgt cgcgcggcaa
     8701 gggcgtgcct gaaccgacgc gcggtgtatt tcggaaagca cgtacgctgc tcgagggcgc
     8761 gaagcggcgc ggcgaggtga tcagggtcaa ggagacgcgc atcggcctgg aaggggagac
     8821 gcgcctctgc gtcgaggcaa aggacgctgc ggcagcgcgc gcgctcctgc gggagctgcg
     8881 cgcgattgcg gaaaacgtcg agctgttcaa tgtcgtagtg gaaccttgtc tgaagaagtg
     8941 aggaatcgtt gactttgcgc aggggaaaac ccggcgtttg agaggtgggt catgaagtac
     9001 tctctcgcgg cgctgccagg cgcatgcgtt cttgcctgca gtttccttct cctcaccggc
     9061 tgtgagcccg ccaccggtgc cgggaacagc gccgataccg agtgcgcgat gccgaccacc
     9121 aaagacgaat tgcagcctcg cgcgagcgag ggtcatgccg ggacgcagat tcgtccggca
     9181 acggagcggg tggaagccac gcccgagacc gtcgcccagg caatggcgca aatggagcgc
     9241 agggcaaaag aagagtgccg gcaatgggcg gacaaggcct tcgaggagtt cgagcggtcc
     9301 acctacaggg agcccttccc gggcggcaag tacatcgtca gcggcgacat cgcgatctcg
     9361 gatcgccagc ggctgcggga attctttgaa acacgcataa agggggaatc gtccgagaca
     9421 gtaccgggtg cgctgatcgt gcatcgggtg ggcggccggg atgcgatctg ggattcgccc
     9481 ctgaggaagc atctgaccta ctgcgtgagc agcgctttcg gcgaccggcg gcaacaggtg
     9541 atcagggaca tggaggccgc ctccggcgcc tgggaagcgg tcgcggatgt ggatttcatc
     9601 cacgtaacgg ccgaggacgc cgactgcacg cccgccaatg gcaacgtcgt gttcgatatc
     9661 cggccagtca acgtggatgg gcaatatctg gcccgggcgt tcttcccgaa cgaagagcgc
     9721 tccgagcgga atttactgat cgacgaatcc tcgttccgtc tggaccccca cggggccttg
     9781 caactggtcg ggatcctgcg ccacgagatt ggccacacgc tcggttggcg gcacgagcac
     9841 acccgcccgg aatccggtac ctgcttcgag gataacaact ggcggccgct taccgactac
     9901 gaccgcttct ccgtcatgca ttacccgcag tgcaatggcg gcgctgactg gtcgctgaaa
     9961 ctgacgcaca tggacaagaa cggagcggcc tgcgtctatg gtgccgcgcc gggcttcaac
    10021 gtggacccgc agatctgccc gcgggggtaa gggcaaggat gaaggataaa ggatgaagga
    10081 tgaaggcgga gggatgaagg aacggcatta ggtgggtgct gcccgttcct actccccggg
    10141 cggatcctgc gtttcctggc cggaggtaag gccgccatgt gctgcgtgcc tgctcgccgc
    10201 cttggcggca atggaagcgg agatctccat cagtgcgcat acgagtgcct cgattcgtgc
    10261 attgagctct ttcggatcgg cgtccaccag acagcggatc ggccagagtt taggcgtggt
    10321 gggtgtgaac ttgtaccgcc cggacggaga ttcgaatcgg atcgtcccca attcgtgatc
    10381 cgattccgat accactaccg tgcccggact gccgctacgc tcatcaaagc gcaaggtggg
    10441 catgcttatc ctccgcttcc gtccccggtg cgatgcaggc agcggacagg cgcgagaatg
    10501 ctgcaaaccg ggtagtgcat ctgtttccgc cgtttgcgcg gtacttcatg gtattttcag
    10561 tgccacgttg cgtacccggg aagtcgtgct atcccggagt ccaggcacag gcgaattcat
    10621 gagtccgctc cccattggag ttgggctcga cgctttccca gatgcgcctc atctttccgg
    10681 acgaccagat acgctcgccg aaaagattga acatgtccat cgagagcacg cgatggtgcc
    10741 tgttcctgca gttctcctcg atcaccccgg ctgtttcctt gaccgtggcg ttgtccgata
    10801 gttgcaccgg ccacagggac acgcggtaaa ccgccttttt gataagggag acgatttcct
    10861 cattgcggct gagcgaatcg gcgtcgacgt aatacgttgc ggtggcgtcg cggtcgattt
    10921 ccttccaatt ctccgcgtgc gcagggacgg cgatcagagc aacggctgca aagaaaaatt
    10981 tcatggcgcc tccttccggt accggcaagg cgggcgggga aaaatccttc aggtccgctg
    11041 ccgcgcgccg gatgattcag ttgatcatgg cggcatgatc cgctggcgac gttatgccga
    11101 ttgcatctgg aggggagtcc gtccggagca tgcccggttc gactttgtag cgatgccttg
    11161 tcaaggcttc cgcacatctc agggaaagaa cttatgaggt cctcagggag ggaccctaaa
    11221 aggaggggcg agggaagggg gatggggaat gagcgattgg gcgattaggt gttgggcgat
    11281 caggtgactg aatcactgaa tccctcaatc actgaatcac tgacgcgggg gattgggcga
    11341 ttaggcgatt gaatcgctga atccctcaat cgctgaatca ctgcggggcg gtgtggtatg
    11401 gttgaaattc ccgtccgagt cccgtggcag gcatgggtcc gaagaactgt cgccacaatt
    11461 atggggagga accagatgat tcattcgttg aaggtggtcg cgcggtcggc cactctcgcc
    11521 gtattggcag gacttacact ggccagcgcg gccggcacgg cggaagcaca gcacaagtgg
    11581 aaagtgcaaa acctctacgg gccgaacacc accgtctaca aggattttca ggcctttgcc
    11641 gcacgggtga aggaggccac caagggcgag gtggaaatcc agcccttccc ggtcgcgacc
    11701 atcgtgccgc aggccgaagc gctcgacgcg atcaaggcag gtttgctcga cggcgccgtg
    11761 gggacgatgg cgtaccaggg cggcaaggag gcagcggcgg cattctggga catgacggct
    11821 gcctatgatt acccgctgca cctgctgatg tggtaccggc aaggcggcgg gatggaggtc
    11881 atgaacaaga tttccgcaaa gtgggacgcc gttttcctgg gcccggtgat ccttgggctg
    11941 gagtctatcc cgtcgaaaaa gccgatccgc tcgatcgccg acttcaaggg cgtgaaaatg
    12001 cgtgcacccg acggtattcc ggcggagatc ttcgccttgc tgggcgcatc ggtctcggtg
    12061 atgcccggca cggaggtgta cacggcgctg gataccggca agatcgaggc gaccgactgg
    12121 ggcacgctgt cggtgaacga cgaggccggc tacaaccgga tcgctcctta tgcgatctac
    12181 ccgggcatcc actccatgaa ctcggtcgac ttcgtcgtca gcaaacgcaa gtggaatgcg
    12241 ttgtccgccg aacagaaggc gatcgtgacg gccgcggtgg acgagtggtg cctgcgcacg
    12301 tggatggacc aggaactcga ggatcgcaag gccgccgcca agcgcgatcc gggcacgctg
    12361 atccagtggg gcgagaagga gaagggcgaa ttccgccagg tttcccggaa ggtatgggag
    12421 aagtggtcga agaaaagccc gctggccaag gagatctacg aaagccagac gaaattcctg
    12481 aaatcgatcg gcaagctata gacatatgca agcaagggac tgagcgaacg ggcgcggctt
    12541 gagccgcgcc cgcttcgttc ccgcggacaa caagaagaag gggggaatca tggcggaatc
    12601 ggcgcccgat gccgaagcga ccgggatagg cgctgtcgat cgcgcgatcg aggcgatcgc
    12661 gaaggcgttg agtttcacct tcgcgctgtc gatcgtcgtg acgatctacg acgtcgtcgc
    12721 cgacgtggtc ttcaagtcgc cgaccgtgtg ggtctacgac gtcgtcacca cggcgatcgc
    12781 cgtggccttc ctcatcggcg gctcctattc gctgatgcgc cgcgagcata tccgcatcac
    12841 cgcggtccac gatcgcttgt cgcggcgcac gaggctgcgc tgcgatatcg ccacctcgat
    12901 tctcgcgagc gtttatctcg tcgcgttcgc ctggttctcc ttcaacatgg cgtggctggc
    12961 ggtccaggac tgggaggtcg gcgggtccgc atgggcgcag cccacaccgg tcatcgtcaa
    13021 ggtttcaatg gtgatcggcg ccctgctgat gatcgtgcag atgatatcca atatccgcgg
    13081 cgacctgaaa gcgctgcgca acgaggcctg agcgatgggc atcgagacca tctcgctgtt
    13141 gatgctcgcg gccttcgtgc tgctgatcat cgtcctgggc atgccgatcg cgttcgcgac
    13201 cgggctgatc gcggtcgtgt tctgcgcgct gttcgccgac ctggacagtc tcgccatcgt
    13261 caccttccgc acttacgggt tcgtcaattc ctatgtgctg ctgtcggtgc cgatgtttct
    13321 gatgatggcc aacatcctcg acaaatccgg agtcgcgcac gatctgtacg aagcgttgaa
    13381 gatctgggcc gggcggcttc ccggtggtgt ggcggtcatg accttgatcg cggcgtcggt
    13441 gatggcggcg atgaccggca tcatcggcgg cgaggtcatc ctgctcggcc tggtcgcgct
    13501 gcctcagatg ctgcggctgc gctacgactc gaagctcgcg atcggcgtgg tatgcgccgg
    13561 cggctcgctg ggaacgatga ttccgccaag cctggtactg gtgttctacg ggcttacgac
    13621 ctcgacttcg atcggagacc tgttcctggc gtccacgatt cccggcatcg tgctggcgct
    13681 gatctacatt atctatgtgc tgatccgctg cgggctcaac ccgaagctcg ggccgccggc
    13741 gccgccggag ctgcgcaaca tccctttgag ggccaaattc gcggcgctga ggagtgtcat
    13801 cctgccgctg ttgatcatct tttccgtgct cggcagcatc tacaccggca tcacgtcggt
    13861 gagcgaggcg gcggcgatgg gtgttgccgg agtgaccgta gcggccgcga tccggcgcac
    13921 gctgaccctc gcgatgctgc gcgactcgct gtattcggcg atgtcgacct gcggcctgct
    13981 gatctggctg tcgatcggcg ccaacgcgat ggtgggcgtt tacaatctcc tgggggggat
    14041 cacttacctg aagagcatca tgaccggcct gccgctggcg ccgatcggcg tcatcctgat
    14101 catgatggcg atcttcctgg tgctcggcat gttcatggac tggttctcga tcatgctgct
    14161 gaccatgccg atcttcgcgc ctacggtgac ggcactgggc tacgacctga tctggttcgg
    14221 ggtcctgttc aacatcacca tgcaggtggg ctacctgtca ccgccattcg gccaggcagc
    14281 gttctacctg aagtctgtgg cgccgcccga gatcagcctc aatcacattt tcgcctctct
    14341 gttccctttc atcgggttgc agatcatcgg cctgatcatc gtgctcttca ttccggaggt
    14401 ggcgatgttc ctgccgcgcc tgctgcacta gctggtgggc aagatcgacc gacccgctcg
    14461 tcgcgccacg ccatatatat agcagcgctc accgggcgag cggatcgggc cggagctgga
    14521 tcttgtaaac cttggcagtg cgttgcgttc cgccttccga atgaaacggg gcccgggtgg
    14581 aatctgtcgc ccacagcgcc cggcctttcc atccggcttt cggatcgtcg atccggcctt
    14641 caccccactt cgcgaagaag cccatggggt agggcacgcg cagcgtcacg aacttgccgt
    14701 ccacgagcgc gaagtacgaa tcgctggtgt tgccggtcgc aatcggcacg ttgttgccga
    14761 gaccgagcgt gttgaaccag tccacccaga cgtagtaact cgcctccgcg ctgccgtcgt
    14821 cctgcacgtc ccggaactgc ggtccgggga accggtacag cgtccagccc tcggggcagt
    14881 gcttgccggt cgcattcggc ccgttcaacg gcgccttgca cttgcgacgg tcgaagctgc
    14941 cgagatgacc gctcgcgaac gacgcccaga acacgccgtt gcggtcgatg tcgccgccgc
    15001 gcgcgccgta ggcgggcaac ggcggttcgt agacctcggt gatcgcagtg tgcgtcggat
    15061 cggatcccgg ggcgacgcgc acggcgtagc tggggaacgg cgccagcgaa gttccccaca
    15121 ccgagccatc gaccggattc acgacgacgc tgtagagatt gaccgcgacg cgcttgtctt
    15181 tcgccgggtc cagcggctgg tcgggctcga cccattcgtc gcgcctgccg ttgcggttgg
    15241 tgtcgaggat gaagggcgtc cagccctgtg acttcatctc gtcaccggtt tcctcgaaca
    15301 ttttccggtt cagccagccg accacacctg ggccggagac cccggccgag gcccacagcg
    15361 tattgttggc atcctcggcg aagatcaagt ggtgcgtcgg gaagcaggtc gagatcagcg
    15421 tccacttgcc ggtggccggg tcgtacatcg acagatgccg gctcgcgctc tcgaccggaa
    15481 acactttcgc cgacggatgg tccgagcctt ttctgcaggc gtcggggttg ttcgggtgcc
    15541 ggatgcgcgc cgcagcccat acccgtccct tctcgtccat catctggttg tgcgtgctgg
    15601 tctggctgtc ccagatcgga tccggacccc agtaaggcga gggcgccatc ggatcggtgc
    15661 ggtgcgaagg cgtattcgga tcgcgcacgg gactcttcac ctccgtcgcg gtgtgcttca
    15721 gcggttcgag gaccggccag taatccgtgc tgttctcggg cgcgccgtag aacttcccgt
    15781 tggtattgac cgttggcatg cgcttgtcgg tcccgatcaa gtcatgcatg taggcgtggg
    15841 gtcggctcca gtcccacagc gtcagcacga gattgcgttc gataccctgc ggccgctcgg
    15901 gcttcgcgaa gggcagctcg ccggcggcga tccggtcggt ccagtcggcc cactgcgcga
    15961 gcgcacgccc ggtaccgagc cggcccgcga tggtgaccat gttgttcatc gcctgtcccg
    16021 aggcgatgcg ccgtccccag gcctcggtgg aattcatatc cgcgaattcc ttcggcatgg
    16081 tgcgggtgcc gacggtgccc aacgcgtgac aagacatgca gccgttggtc ttgatggtgt
    16141 tgagccagtg ggtctgcgtc ttcatgcccg ggtcgatgcc gttgccgtcg attcccgtgc
    16201 ctggaaattc gcttttcgcc ggtatcttca gcagcgagta ccagtagatc gccgggtaat
    16261 attccgcggc cgcggcagcg ctcggcgcca ccaccgcgct caagtcgaga cgtttgccgg
    16321 gcgtggcctg gactttgggc gaatcgacca gcccgtaacc gcgcacccag acgctgtaac
    16381 tcgccttcgg cagatcgggc atgaggtagc gcccttgatc gtcggtaacc acgatcttgg
    16441 caaatctggt gggtaggtcc gaggtttccg cgatcaccca tacaccggct tccggtccgt
    16501 tcgtgctctt caccactccg ccgagatcgt tgtcggcgat gcggatcttc gtgctgtcgt
    16561 cgcataagcc ggtcccggtt gcgcagacag cgagcagcag cgcgattgcg gtcgccgcga
    16621 ttccgcggcg taatgctcca gtcgtcatgt tggtgctccc ctcctgatat cgctcgatgg
    16681 tattgggcga aacattgtag aaccgaaggg aagccatggg gtgaagtctc ccattattca
    16741 tccgctcatc gcgccgcatt gtgtgcaaca tggcagaatt gatccct
//
LOCUS       DASQOP010000122         7216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_250007, whole genome shotgun sequence.
ACCESSION   DASQOP010000122 DASQOP010000000
VERSION     DASQOP010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7216
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250007"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            359..1375
                     /locus_tag="VE030_04885"
     CDS             359..1375
                     /locus_tag="VE030_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZF22787.1"
                     /translation="MSYTVERATRASRIGGVLALILVAFLATFPFWADHGWMREFNEM
                     ACYLIFAMMWNLLAGYGGMVSIGQQAFLGIGGYALLVMGNFLDVNPFVAVPLAAAFTM
                     LVALPVSKVAFRLQGGYFAIGTWVIAEVFRLSIANVSAVGGGSGTSLTALRGIEKATR
                     VSLTFWLCLTGAVTSVALVYAFLRSRHGLALLAIRDSEIASESQGVNVRATKLGVYLV
                     AAFGAGLAGALYFLGNLRISPDAAFTVNWTAFSIFIVLIGGIGTIEGPIVGVILFYLL
                     SKFFSDYGTWYLVGLGLLAIVVTIRFPRGLWGWLSNKYDLHFFPVQRRLVLPPDMRTD
                     QHRN"
     gene            1385..2587
                     /gene="pcaF"
                     /locus_tag="VE030_04890"
     CDS             1385..2587
                     /gene="pcaF"
                     /locus_tag="VE030_04890"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HZF22788.1"
                     /translation="MAEAFICSALRTPIGRYGGALAMVRPDDLAAVPLRALMERHKSV
                     DWNALDDVVFGCANQAGEDNRNVARMALLLAGLPKEVPASTINRLCGSGMDAVGIAAR
                     AIKSGEAQLTVAGGVESMSRAPFVMPKADTAFSRTNAVYDTTIGWRFVNPAMKQLHGT
                     DSMPETAENVAAEFKVSRADQDAFGLRSQARASSAQKNGRLAKEIVAVTIPARKGDAT
                     VVDKDEHPRETTLEALAKLKPIVKPEGTVTAGNASGVNDGACALILASEAAARRHGLT
                     PLARVVGIATAGCAPRIMGMGPVPATQKLLERTGIKLDAIDVIELNEAFAAQGIAVLR
                     QLGIRDDDARVNPNGGAIALGHPLGMSGARLVATATYELHERKARYALTTMCIGVGQG
                     IAALIERI"
     gene            2635..4368
                     /locus_tag="VE030_04895"
     CDS             2635..4368
                     /locus_tag="VE030_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZF22789.1"
                     /translation="MATHKQKEKKQGASRRDFLKVAGAGTAAATFGALTLSAKEAKAQ
                     AFDAEYDIVVCGGGGAGLPVALFSRWLGNKVIVLEKAATLGGTSFKAAYWYWVPNNAA
                     MRKAGIPDDKQHFLRYVARLSQPQNFDLNHPRYGLSEWEYSMCEAIYDSSSVGTELLS
                     EKGALPYRHVAPVPDYFAEMETIGATGRVLFPKDGAPSMSDGGQVATRTLSTACRRDG
                     VTIRIGHRVQKVIRNDRGEVIGVEAKNDDGVIRIKARKAVIFATGGFTHDAELRKNFL
                     SIPAYGGCAAMTNEGDFVRISSTLGVQLRNMNYAWMCPISFEKAIAKDPSLIGCFSVA
                     GDSMIFVDKRGKRVVNEKLHYNELAQKFFEWEGANAQFPYLILTSIWDQRAQDNSASH
                     EYGRLIVPPGIDDRHVIKGATLEELAANIDTRLQKYAGQNGGMRITSDFVPNLKASIA
                     RFNEFARTGKDLDFHRGERGVDLLFNGNVKEEPGRKNPTMWPISDAGPYYAALVTGGT
                     LDTKGGPKTNPEGQVLDLADKPIPGLYGVGNCVASASGRAYWAGGGTLGPIIGFAHRA
                     ANQANKEPVRG"
     gene            4430..4870
                     /locus_tag="VE030_04900"
     CDS             4430..4870
                     /locus_tag="VE030_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HZF22790.1"
                     /translation="MDSFFESEHWEGMMSKFVAAVKAVRIGAVVTTFVATATPLFAAD
                     DLAAAKEYTVRYCIQCHTFEQGDKHGQGPNLFGLIGREAASAPGFVYSPGIKEALKGK
                     VWDRDLLDAWLTDTLAVAPKAQMIYFQDDPKARAKLIRYLESLK"
     gene            4906..5766
                     /locus_tag="VE030_04905"
     CDS             4906..5766
                     /locus_tag="VE030_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007730166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HZF22791.1"
                     /translation="MSNLTEQNLVNAVLEKLEGAKDPRFKQVMTSLIKHLHAFVRETE
                     LTEAEWFRGIQFLTATGKKCDDKRQEYILLSDTLGVSMLVDAINHRKPGGATETTVLG
                     PFYVSGAKDVPMWADIAGDAPGEPAYVFGRVLDVGGKPIAGATIDVWQTDGEGFYDVQ
                     RPGGDESYARGKFKTVADGRYGFRTVKPVSYPVPTDGPVGKMLLGMGRHPYRPAHVHA
                     IVSAPGYEKVATHLFVKGDTYLDSDAVFGVKQSLVVDFKPHAAGPTPDGGKSAAPFCT
                     AEYDFRLVRT"
     gene            5766..6158
                     /locus_tag="VE030_04910"
     CDS             5766..6158
                     /locus_tag="VE030_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZF22792.1"
                     /translation="MGGWRDLPSAPAPGTVLCALEEIAEGGGKEVVFGTGHEAFRVML
                     LRTGAGVVAYHNCCPHFSLPLNYEPDVFHIFDGDSLMCAHHTAMFKIATGECFDGPCA
                     GAKLTAIPIAVEGETVVIRESGPGQSRI"
     gene            complement(6155..7141)
                     /locus_tag="VE030_04915"
     CDS             complement(6155..7141)
                     /locus_tag="VE030_04915"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZF22793.1"
                     /translation="MRVGLAGAGAISQYHLVGWSEMADARVVAICDTDEAKARAKAQA
                     FSIPKVYTDFRAMIGQEKLDAVDIVTPVGSHAPLTRLAADAGLHVCCQKPLTPTVKEA
                     EELIAYVGDRVRFMVHENYRFRPHYVRVKQWLDGGRVGDILHARMVVRGQSMIEVNGV
                     PPFLLNRQPYLKDFRRLLIFEVLIHNLDALRALLGPLEVVATQVARVNKELAGEDVAV
                     ILMRGRGGLGVVLDGSISAPGYGPLPVDRLEIMGSKGTLIYDRDRLYMVGSDKPPEMF
                     DLAKNYQVCFSSAVREFVQGIRSGNPFPTDRLDNLETLKLMESCYVAAGVKF"
BASE COUNT         1373 a         2192 c         2340 g         1311 t
ORIGIN      
        1 cggcacgctc gccggcgcca tcattctcgg cgtcacccag gccatcggct tccgcttcga
       61 tccgggctgg ggaatctggt tcggccacat cgtttttctg gcgatgctgc tgttccggcc
      121 ttcaggtctt tttcccaaaa cccgctagcg cggatcaagg gcgtcaacgc aaaggacgca
      181 agaggaaaga aagttcgcaa agaaggacgg caatgatgaa ttttgaaaga gaacgcgatg
      241 acgaaagcag gcaaatacgc acctcgaaga gctgtgtgga ccagcattgt tttcacctcc
      301 atatttctga atttctttgc gttgcgtcga gggctggatg acgtcgcgag cgacagccgt
      361 gagctacacg gtagagcggg ccacgcgggc gagccggatc ggcggcgtgc tggcgctgat
      421 cctggtggcg ttcctcgcga catttccgtt ctgggccgat cacggctgga tgcgcgagtt
      481 caacgagatg gcctgctatc tcatattcgc gatgatgtgg aacctgctgg ccggttacgg
      541 cggcatggtc tcgatcgggc agcaggcctt tcttggaatc ggcggctacg cgctgctggt
      601 gatggggaac ttcctggatg tgaatccgtt cgtggcggtg ccgctcgcgg cggcgttcac
      661 gatgctggtc gcgctgcccg tatccaaagt ggcgttccgc ctgcagggcg gttatttcgc
      721 catcggcacc tgggtgatcg ccgaagtgtt ccggctctcg atcgccaacg tctccgcggt
      781 gggcggcggc tcgggcacca gcctgactgc gctgcgcggc atcgaaaagg cgacgcgcgt
      841 atcgctcacg ttctggttgt gcctgaccgg cgcggtgacc tcggtggcgc tggtctacgc
      901 cttccttcga tcgcggcacg ggctggcctt gctggcgatc cgggacagcg agatcgcatc
      961 cgagagccag ggcgtcaacg tgcgggcgac caagctgggc gtgtacctgg tcgcggcatt
     1021 cggcgcagga ctcgccggcg cgctctattt tctcggcaac ctgcgcattt ccccggatgc
     1081 cgcgttcacg gtgaactgga ccgcgttctc gattttcatc gtgctgatcg gcggcatcgg
     1141 caccatcgaa ggtccgatcg tcggcgtgat cctgttttac ctgttgagca agttcttttc
     1201 cgactacggc acctggtacc tggtcggcct gggattgctc gcgatcgtcg tgaccatccg
     1261 ctttccgcgc gggttgtggg gctggctctc gaacaaatac gatctgcatt tctttccggt
     1321 tcagcggcgg ctggttttgc cgccggacat gcgaaccgac caacacagaa actgaggaaa
     1381 cggcatggct gaagcattca tctgttctgc gctacggact ccgatcggcc gttacggcgg
     1441 cgcgctggcg atggtccggc ccgacgatct cgctgccgtg ccgctgcgtg cgctgatgga
     1501 gcggcacaaa tcggttgact ggaacgccct cgacgacgtg gtgttcggct gcgccaacca
     1561 ggcgggcgag gataatcgca atgtcgcgcg catggcgctg ctgcttgcag ggctgccgaa
     1621 ggaagtgccc gcaagcacca tcaatcgcct gtgtggctcg ggaatggacg cggttggcat
     1681 cgccgcccgc gcgatcaagt ccggagaagc gcagctcacc gttgccggcg gggtggaaag
     1741 catgtcgcgc gcgccgttcg tgatgccgaa ggcggatacc gcattctcgc gcaccaatgc
     1801 ggtgtacgac acaaccatcg gctggcgctt cgtcaacccg gccatgaaac aattgcacgg
     1861 aaccgactcg atgcccgaga ccgccgagaa tgtcgcggcg gaattcaagg tgtcgcgcgc
     1921 ggaccaggat gcgttcggtt tgcgttcgca ggcccgggcc tcgagcgccc agaaaaacgg
     1981 gcggctggca aaggagatcg tcgcggtgac aatacccgct agaaagggcg atgccacagt
     2041 cgtggacaag gacgaacacc cgcgcgagac cactctcgaa gccctggcca agctcaagcc
     2101 gatcgtgaag ccggagggca cggtcaccgc cggcaacgcg tccggggtga acgacggcgc
     2161 ctgcgctctg atactcgcca gcgaagcggc agccaggcgg cacgggctca cgccgctggc
     2221 gcgcgtggtc gggatcgcga ccgcggggtg cgcgccgcgc atcatgggaa tgggcccggt
     2281 gcccgcaaca cagaagctgc tggagcgcac gggcatcaag ctcgacgcga tcgatgtgat
     2341 cgaactgaac gaggctttcg ccgcccaggg catcgcggtg ttgcggcagc tcggcatccg
     2401 cgacgacgat gcccgcgtca atcccaacgg gggcgccatc gcgctcggcc atccgctggg
     2461 catgagcggc gcgcgcctgg tggccaccgc aacttacgaa ctgcacgagc gcaaagcgcg
     2521 ctatgcgctg accaccatgt gcatcggtgt ggggcagggc attgccgcgc tgatcgagag
     2581 gatctgattt ttcgatgcaa gacgtttcgc tgtcaactca cggaggagga aaaaatggct
     2641 acgcataaac agaaggaaaa gaaacaaggt gcatcacggc gcgattttct gaaagtcgcc
     2701 ggggcgggta cggcggccgc aacgttcgga gccttgaccc tgagcgcgaa ggaggcgaag
     2761 gcgcaggcgt tcgacgcgga atacgacatc gtcgtctgcg gtggcggcgg ggcggggttg
     2821 ccggtggcgt tgttctcgcg ctggctcggc aacaaagtca tagtgctgga aaaggcggcg
     2881 actctggggg gcacatcgtt caaggcggcc tactggtact gggtacccaa taatgccgcc
     2941 atgcgcaaag ccggcattcc ggacgacaag cagcacttcc tgcgctacgt cgcgcgcttg
     3001 agccagccgc agaacttcga tctcaaccat ccccgctatg gcctttccga gtgggaatac
     3061 agcatgtgcg aggccattta cgacagctcg tcggtgggca cggaactgct gtcggagaaa
     3121 ggcgcactgc cgtaccggca cgtagccccg gtgccggact acttcgcgga aatggagacc
     3181 atcggcgcga ccggccgcgt gctctttccc aaggacggcg cgccctccat gtccgacggc
     3241 ggacaggtgg cgacccgcac tttgtccacg gcgtgccggc gcgacggtgt gaccatccgg
     3301 atcgggcacc gggtgcagaa ggtgatccgc aacgacaggg gcgaggtcat cggagtcgag
     3361 gccaagaacg atgacggcgt gatccgcatc aaggcccgca aggccgtgat cttcgcgacc
     3421 ggcggattca cccacgatgc ggaactgcgc aagaacttcc tctccattcc ggcctatggc
     3481 gggtgtgccg caatgaccaa cgaaggcgac ttcgtgcgga tttcgagcac cctcggcgtg
     3541 cagttgcgca acatgaacta tgcgtggatg tgcccgatct cgttcgagaa ggcgatcgcc
     3601 aaggatcctt cgctgatcgg ctgcttctcg gtcgcgggcg attcgatgat tttcgtggac
     3661 aagcgcggca agcgcgtggt caacgaaaag ctgcattaca acgaactggc gcagaaattc
     3721 ttcgagtggg agggcgccaa cgcacagttc ccttatctca tcctgacttc gatctgggat
     3781 cagcgcgctc aggacaattc ggcaagccat gaatacggcc gtttgatcgt gccgcccggc
     3841 atcgatgacc gccatgtcat caagggtgcc acgctggagg aactcgccgc gaacatcgac
     3901 acgcgcctgc agaaatacgc gggccagaac ggcggcatgc gcattacttc cgacttcgtc
     3961 cccaatctca aggcgagcat cgcgcgcttc aacgaattcg ccaggacggg caaggacctc
     4021 gatttccatc gcggcgagcg cggcgtcgat ctgctgttca atggcaacgt caaggaggag
     4081 ccggggcgca agaacccgac catgtggccg atcagcgacg cgggaccgta ctacgcggcg
     4141 ttggtcaccg gcggcacgct cgacaccaag ggcggaccga aaaccaaccc cgagggccag
     4201 gtgctggacc tcgcggacaa gccgattccc ggtctctacg gcgtgggcaa ttgcgtggcc
     4261 tccgcatcgg gtcgcgccta ttgggcgggt ggcggcacgt tggggcccat catcgggttc
     4321 gcgcatcgcg cggcgaacca ggccaacaag gaaccggtga ggggctgatt gcaggcgaac
     4381 ggagtcgggg cgggtgcatc cgccccggcg tcccgaacgg gcggaagtca tggattcatt
     4441 cttcgaatcg gaacattggg aggggatgat gagtaagttc gttgccgccg tgaaggcggt
     4501 caggattggc gcagtcgtca cgacgttcgt tgcaacggcg acaccattgt tcgcggcgga
     4561 cgatctggcc gcggcgaagg agtacaccgt tcgatactgc atccaatgcc atacgttcga
     4621 acagggagac aaacacggcc agggcccgaa cttgttcggg ttgatcgggc gcgaggcggc
     4681 gagcgcgccc ggatttgtgt attcgccagg catcaaggaa gcgttgaaag gaaaggtgtg
     4741 ggatcgcgac ttgctcgacg cctggctgac cgataccctt gccgttgcgc ccaaagcgca
     4801 gatgatctac ttccaggacg atccgaaggc acgcgcgaaa ctgattcgtt atctcgagtc
     4861 gctgaaatag atgtgcgcgt aaaggacctt cacggagaat tacccatgag caatctgacc
     4921 gaacagaatc tcgtcaatgc ggtgctggaa aaactggaag gcgcgaagga tccgcgcttc
     4981 aagcaggtca tgaccagcct catcaagcac cttcacgcct tcgtgcgcga gaccgaactg
     5041 accgaagccg agtggttcag aggcatccag ttcctcaccg ccaccgggaa gaagtgcgac
     5101 gacaagcgtc aggaatatat cctgctctcc gacacgctgg gcgtgtcgat gctggtggat
     5161 gcgatcaatc acagaaagcc cggtggtgcg acggaaacca cggtgctggg cccgttctac
     5221 gtttcgggcg cgaaggacgt gccgatgtgg gccgatatcg cgggagatgc gcccggcgag
     5281 cctgcctacg tcttcgggcg cgtgctcgat gtgggcggca aaccgatcgc cggcgcaacg
     5341 atcgacgtct ggcaaaccga cggcgaaggt ttctacgacg ttcaacggcc gggcggagat
     5401 gagtcctatg cgcgcggcaa attcaaaaca gtggccgatg ggcgctacgg ttttcgcacc
     5461 gtgaagccgg tcagttaccc ggttcccacc gacgggccgg tgggcaagat gctgctgggg
     5521 atgggccgcc acccctaccg tccggcccat gtgcacgcga tcgtttccgc gccgggatac
     5581 gagaaagtgg ccacccacct gttcgtaaag ggcgatacct acctggattc cgacgccgtt
     5641 ttcggcgtga agcaatcact ggtggtggac ttcaagccgc acgcggcggg tccgacaccc
     5701 gacggcggga aatccgccgc gccgttctgt accgccgagt acgacttcag gctggttcga
     5761 acttgatggg cggctggcgc gacttgccct cggcgccggc gccgggcacg gtattgtgcg
     5821 cgctggaaga aatagcggaa ggcggcggca aggaagtggt gttcggtacg ggccatgagg
     5881 cattccgtgt aatgctgctg cgcaccggcg cgggtgtggt cgcgtaccac aactgctgcc
     5941 cgcacttttc gctgccgctc aactatgagc cggacgtttt ccacatcttc gacggcgaca
     6001 gcctgatgtg cgcgcaccac acggcaatgt tcaagattgc caccggggaa tgtttcgacg
     6061 gcccctgcgc cggcgcgaaa ctcaccgcga ttccgattgc cgtcgagggc gagaccgtgg
     6121 tgattcggga aagtggcccg ggacaatccc gaatctagaa cttcacgcca gctgcgacgt
     6181 agcaggattc catcagcttc agcgtctcca gattatccag tcgatcggtc gggaagggat
     6241 tgccgctgcg gattccctgc acgaactcgc gcacggcgct gctgaagcac acctgatagt
     6301 ttttcgcgag gtcgaacatt tccggcggct tgtccgagcc gaccatgtag agccggtcac
     6361 ggtcataaat cagcgtgcct ttgctcccca tgatttccag ccggtccact ggcaatggtc
     6421 catagccggg cgccgagatg ctgccgtcca gcaccacacc aagcccgccg cggccgcgca
     6481 tcaggatgac cgctacgtcc tcgccggcaa gctctttgtt gaccctggcg acctgggtcg
     6541 cgacgacttc gagcggcccg agcagggcgc gcagggcgtc gagattgtgg atcagcacct
     6601 cgaaaatgag gaggcgccga aagtccttga gatagggctg gcgattgagc agaaatggcg
     6661 gcacgccgtt gacttcgatc atgctctggc cgcgcaccac catgcgcgcg tgcaggatgt
     6721 cgcctacgcg cccgccatcg agccactgtt tcacccgcac atagtgcggc cggaagcggt
     6781 aattctcgtg caccatgaag cgcacccgat cgcccacgta ggcgatcaat tcttcggctt
     6841 ccttcaccgt cggcgtaagc ggtttctggc agcacacgtg cagtccggca tcggccgcca
     6901 gtcttgtcag cggcgcgtgg ctgccaaccg gcgtgacgat gtccaccgcg tcgagcttct
     6961 cctgcccgat cattgcgcgg aaatcggtgt agaccttggg gatgctgaat gcctgcgcct
     7021 tggcgcgcgc cttggcttca tcggtatcgc aaatcgccac gacccgcgca tccgccattt
     7081 ccgaccagcc gaccaggtga tactgactga tggcgccggc gccggctaaa cctacgcgca
     7141 atttttccac cgtgcctccc ctcgttgccc aatacagggc ttcacaaaac gaagaccccg
     7201 ctggcttcgc ggggcc
//
LOCUS       DASQOP010000123        11820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2266081, whole genome shotgun sequence.
ACCESSION   DASQOP010000123 DASQOP010000000
VERSION     DASQOP010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11820
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2266081"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VE030_04920"
     CDS             <1..532
                     /locus_tag="VE030_04920"
                     /inference="COORDINATES: protein motif:HMM:NF024562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3999 family protein"
                     /protein_id="HZF22794.1"
                     /translation="ENTIVPVRFLSRGKPEDRWTPVTHTVAYRLNQNGRELVSPDIAV
                     GVNSHRYWLLRVDPAAGGIGPGSLIVRAGWTPREIVFTARGTGPFRLAYGNSKAQAGS
                     LGIEALVPGWRTDQVPKMSVAATGPVQKLAGEAAALPRADLKKWGLWSALFAGVAVLA
                     WMAWRLSKQMQRPDTK"
     gene            complement(586..876)
                     /locus_tag="VE030_04925"
     CDS             complement(586..876)
                     /locus_tag="VE030_04925"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HZF22795.1"
                     /translation="MTRLVLSPKAVDDIDRLSDFLLDSDPSTASKTGGILVSGLQILK
                     DHPLIGRQVERGFQELLISRGRTGYVALYRFDVVKDIAIVLSVRHQREAGYI"
     gene            complement(886..1167)
                     /locus_tag="VE030_04930"
     CDS             complement(886..1167)
                     /locus_tag="VE030_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22796.1"
                     /translation="MSASTTLKLPEKLKRRITTLAKAAGKTPHAFMIDALETQAVLAE
                     RRREFVASAIAAEEEVAQYGLVYDADEVFSYMKAKLDGRRARRPKPAKL"
     gene            complement(1236..1544)
                     /locus_tag="VE030_04935"
     CDS             complement(1236..1544)
                     /locus_tag="VE030_04935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HZF22797.1"
                     /translation="MIHVIATVELAPGTREKFLAEFRKLIPAVKAEAGCIEYGPTIDA
                     DTDIAIQAKMGPDRVAVVEKWETVAALKAHGVAPHMQAYRAKVKDFVKGMELRVLKPV
                     "
     gene            complement(1833..2195)
                     /locus_tag="VE030_04940"
     CDS             complement(1833..2195)
                     /locus_tag="VE030_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22798.1"
                     /translation="MASKARYIFIASMDVDSEKEDLFNEVYDTEHIPLLLKVPGVISA
                     KRYKTEPLTVFVRGAKQTVVHEDEPRYSAVYEIESPKILVSDAWAKAVDKGRWPTEVR
                     PYVRNRRLVLRKLLEPGS"
     gene            complement(2200..2955)
                     /locus_tag="VE030_04945"
     CDS             complement(2200..2955)
                     /locus_tag="VE030_04945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZF22799.1"
                     /translation="MGAHNKIAIVTGAGSGIGKSVALTLLKDGYSVALAGRRKDALEQ
                     TVKESGAGTRALAVPTDVSDPQSVKALFAKAKEAFGRLDLLFNNAGVNAPGIPLEDLT
                     VEQWRNVVNINLSGMFFCIQEAFRTMKNQNPRGGRIINNGSISAHAPRPNSSPYTSTK
                     HAVTGLTKCASLDGRKYDIACGQIDIGNALTEMAARMTKGVPQANGEIKVEPTMDVQR
                     VADAVLHMASLPLDTNMQFVTIMATKMPFVGRG"
     gene            3084..3857
                     /locus_tag="VE030_04950"
     CDS             3084..3857
                     /locus_tag="VE030_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22800.1"
                     /translation="MPIGRILGAVLATLLTAIGPAAADALKDDAIARLAASHEPDVPM
                     AVGRLAAKQAGLGAARTLLARRGRDAGLGRGWNAAAPEWQDAEAHFSRIVDGIIARGI
                     EDPDWLHTIWADQAARVLNAEEADEIATHFATPVGREQRIVIEVKVVGELLLTSYTFT
                     DRISDRVPGSEREFARMQAIWAEREPFKVRNFDGDAGAARFGSRNPGVKYVRMLAIQG
                     VDAMLGHINAVCAEAVLAVSRAAAQADPFIESYRRRTGN"
     gene            complement(3869..4558)
                     /locus_tag="VE030_04955"
     CDS             complement(3869..4558)
                     /locus_tag="VE030_04955"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HZF22801.1"
                     /translation="MDTGSLFEHLRRFDTPTIANALEIARGQRTITGFTRQTLIAAFP
                     QLPPIVGFARTVTIRSSTPYEPALRRKNQLAYYEYLAQPEQPTVAVVQDIDSQPGLGA
                     FWGEVNTHIHRGLGCVGAVTNGSMRDLDAMHPQFQCLAASLSPSHAWVQVVDFGRQVD
                     VSGLVVSDGEVVHADRHGAIAIPRDLLDKLPAAIDLMARREKVLLDASRKPGFTMATL
                     RQAFAASDQIK"
     gene            4688..5317
                     /locus_tag="VE030_04960"
     CDS             4688..5317
                     /locus_tag="VE030_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22802.1"
                     /translation="MLHTGNYLADQLLIPFVLWFFIVVAVVGIALGVCLIASSARTLR
                     FLGTTNRWISLRTRFRPMEAPHDIGKAIYGHRRWFGAAFVVGGAFMMFMLLASVKGAA
                     VVSALGRSATPAVVAWIVESLRWILLAGGALAVVTGVMLVFSSDALRALEARTNRWYS
                     ASQLGKGADTMYLTLDQWVETFPRTAGWMLGLGAAIVLIASIIVWFGQR"
     gene            5772..6143
                     /locus_tag="VE030_04965"
     CDS             5772..6143
                     /locus_tag="VE030_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22803.1"
                     /translation="MNTESASDEVTDSIAHIEMHRQEMAKEASLASACSEALRKLHEL
                     LKHEQEELQKNGPHSQHTANIAALTGAIEKVKRLTGVKNAGPPSKDRRPEGRRQTPMR
                     GANPGATRNKGRRTMGRRGER"
     gene            complement(6341..7507)
                     /locus_tag="VE030_04970"
     CDS             complement(6341..7507)
                     /locus_tag="VE030_04970"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZF22804.1"
                     /translation="MDFSFTPDQLAIKEAIAKLCARFDALYWLERDRSGGFPEDFYRA
                     VAESGWLGIAMPQQYGGAGLGITEAALMMQAVAESGAGFSGASALHMNIFGLNPVVVF
                     GTDEQKKRWLPALIRGEQKACFAVTEPDAGLNTAKLATSARREGSHYFLRGQKIWIST
                     AQVADKMLILARTAAAQGGGKPTDGLSLFYTDLDRRYVEVREIEKMGRNAVDSNMLFI
                     EELPVPVQDRIGEGGKGFDCILHGLNPERILIAAEAVGLGHAALARAVAYANERVVFD
                     RPIGQNQAIQHPLARCWMELEAANLMVFKAAALYDKDEPCGAESNAAKYLAAEACFSA
                     CEQALLTHGGMGYAKEYHIERYLREAMIPRLAPVSPQLILCFIAEKVLGLPKSY"
     gene            complement(7530..8720)
                     /locus_tag="VE030_04975"
     CDS             complement(7530..8720)
                     /locus_tag="VE030_04975"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZF22805.1"
                     /translation="MPGPLSGVRVLDLTTVVMGPFATQLLAELGADVIKVEAHEGDNM
                     RHPAPMKTPGMGYMFLNLNRGKRGIVLDLKKPEGRKALMRLIPATDVLIYNVRPQAMA
                     RLGLSYEEVRAANPRIIYVGTYGYSQRGPYAAKAAYDDLIQGGTAIPWLVSKTGAKAP
                     NYTPVNLADRVTGLHAVYAVTAALFHRSRSGLGQSVEVPMFESLAHFVLGDHSAGLTF
                     DPPAGDAGYARLLARRPYPTKDGHLCVLVYNDKQWKTFTDATGRPEIMQDARFATQAS
                     RARHISAIYATLAELFLTRTTSEWIELLEKADIPVARLNSVEDLVADPHLAATGFFTL
                     EAHPSEGIVRTMRTPTDWSESQPEGQRPAPRLGEHSAEVLREAGYNETEITELANKGV
                     TRLG"
     gene            complement(8735..9691)
                     /locus_tag="VE030_04980"
     CDS             complement(8735..9691)
                     /locus_tag="VE030_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZF22806.1"
                     /translation="MRLVTFTLAIVFSAPSPQALAEAYPSKPVKLVIAFAAGSATDSA
                     GRILAQELSQRLGQNVIVENRAGANGQIAAVFVARSTADGYTLFMTTNSTHGANPHLY
                     KKLAYDPIKDFEPIARVGTLPFMLVVNPELPANNTAEFIAYAKAHPGGLSYGTASTAS
                     LVGAETINHLAGLDLVRVGYSSSPQAILDLVAGRLQVMVADFTTAMPHVKAGKLRVLA
                     VTTAKPTTLLPEVPPIAATLTGFDLTSWNGIFAPAGTPKEIVARLERDTLEILSRSEI
                     RKRFAAIGFEIDPMNTSEFSRYVREQIAYWGKLVRDAGIPPE"
     gene            complement(10033..10422)
                     /locus_tag="VE030_04985"
     CDS             complement(10033..10422)
                     /locus_tag="VE030_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZF22807.1"
                     /translation="MSLPIPSRYFVRPQDMPPYHPANHTGTVNRRLIGAGNVGAAQVE
                     VVLGVIEKGKGALPHSHSGIEQVCYLLEGRARAEVGGEVCELGPGDCCFFPPGMRHTF
                     TVVSDEPVKVLVIYSPPYGEQPAHGRQ"
     gene            complement(10487..11269)
                     /locus_tag="VE030_04990"
     CDS             complement(10487..11269)
                     /locus_tag="VE030_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HZF22808.1"
                     /translation="MQRIVNPAKELLSKGAIAIGIGVRAVRGVEIARVMKTAGFDWLF
                     IDLEHGPTSVETAYSISVAALDAGIAPLVRVPAGELALATRCLDGGALGIVMPHVDTP
                     EQARAMVDAFRFTPLGHRSIAGGYPQFGFAAAPPNEVVAEMNQATLVVAMLETPRAIE
                     NAEKIAAVPGIDVLLIGTNDLCLEIGIPGQLEHERVVAAIDAVTGACRKHGKWPGLGG
                     VYTKDLLKRYIGRGMRMILAGNDLGLLLGAARDQASFVRSCL"
     gene            complement(11660..11803)
                     /locus_tag="VE030_04995"
     CDS             complement(11660..11803)
                     /locus_tag="VE030_04995"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22809.1"
                     /translation="MQGEAFSRAIRGEISLPYGVEDAVQSMRVIDALFRSEKSGRWEE
                     VQG"
BASE COUNT         2137 a         3656 c         3713 g         2314 t
ORIGIN      
        1 ggaaaacacc atcgtgcccg tccggtttct gtcccgtggc aagcccgagg acaggtggac
       61 cccggtgacg cacacggttg cctatcgcct gaatcagaac ggacgcgagc tggtcagccc
      121 ggacatcgcg gtcggcgtga attcacatcg ctactggctg ttgcgcgtgg acccggcggc
      181 cggcggcatc ggcccgggat cgctgatcgt gagggcaggc tggacaccgc gcgagatcgt
      241 tttcacggcg cgcggcaccg gcccgttccg gctggcctac ggcaactcga aggcgcaggc
      301 gggatcgctc ggcatcgagg ccctggtgcc gggctggcgc accgaccagg tgccgaagat
      361 gtccgtggct gcgacgggcc cggtgcagaa actcgccggc gaagcggcgg cgctcccgcg
      421 cgccgacctg aagaaatggg gtctgtggtc ggcgttgttc gcgggcgtgg ccgtgctcgc
      481 gtggatggcg tggcggctgt cgaagcagat gcagcggccc gacacgaagt aacgaaacag
      541 atcgtgcgcg taacgatgct ggcaactacc tcgatcccgg cttcttcaga tatagccggc
      601 ctcgcgctga tggcgaaccg agaggacgat ggcgatgtcc tttactacgt cgaatctgta
      661 aagtgccaca tagccggtgc ggccacgtga gattaggagt tcctggaaac cgcgttccac
      721 ttggcggcca atcaggggat gatctttcag gatctgaaga ccgctgacca ggataccgcc
      781 tgtcttgctt gcggtgctcg gatccgaatc cagcaggaaa tccgacagcc ggtcgatgtc
      841 gtcaaccgct ttgggcgaca ggacgagacg ggtcacgcgc aatgctcata gctttgcggg
      901 tttgggtcgc ctggctcgcc ggccatcgag ctttgccttc atgtaactga aaacctcgtc
      961 ggcgtcgtaa accaatccgt actgcgccac ttcctcttct gcagcgattg ccgacgcgac
     1021 aaactctctg cgacgttccg caagcactgc ctgtgtctcc agagcgtcga tcatgaaagc
     1081 gtgtggtgtt ttaccagccg ctttagcgag cgtcgtaatg cgacgcttga gcttttccgg
     1141 gagttttaac gtcgttgatg cagacataac cagtcctcaa tgaagtgtta caaaagtaac
     1201 accggagact ctcgggagtc aagcgtggtg cccgctcaaa ccggtttcaa cacccgcagt
     1261 tccataccct tgacgaagtc cttcaccttg gcgcggtagg cctgcatgtg cggcgcgacg
     1321 ccgtgcgcct tgagcgccgc gacggtttcc catttctcca ccacggccac gcggtccggg
     1381 cccatcttcg cctggatggc gatgtcggtg tcggcgtcga tggtcgggcc gtactcgatg
     1441 cagccggcct cggcctttac ggcagggatg agcttgcgga actcggccag gaatttttct
     1501 cgcgttccgg gcgcgagctc gactgtggca atgacgtgaa tcattttttt cctttggctg
     1561 gttggggaca tccgtgggcg cctggtgcgt ccgcaacaaa gcataaaacc gctcatacca
     1621 cggaagacac ggagaacacg gaggaaaacc aacaacgagg aagggcaata tgggataaac
     1681 ggcgaagtga atgtacttca tcctctgccg ccaattcttg ctcccgcaga ctgttcgtcc
     1741 agtgaaggtc ttcactatcg aataccttat ttctcttgtc cttcctccgt gtactccgtg
     1801 tcctccgtgg tgagcgacgc agtgtggtgg ggtcacgacc cgggttcgag cagcttgcgc
     1861 agcaccaggc ggcggttgcg aacgtaaggc cgcacctcgg ttggccagcg gcccttgtcc
     1921 accgctttgg cccaggcgtc gctcaccaga atcttcgggc tttctatttc gtaaacggcg
     1981 ctgtagcgcg gctcgtcctc atggacgacg gtctgtttcg cgccgcgcac gaacaccgtc
     2041 agcggttcgg tcttgtatcg cttcgccgaa atcacgccgg gcactttcag aagcagcggg
     2101 atatgttcgg tgtcgtagac ctcgttgaac aagtcttcct tttccgagtc cacgtccatg
     2161 ctggcgatga agatgtagcg cgctttggag gccatggctt cacccgcgtc cgacgaaggg
     2221 catcttggtg gccatgatgg tcacgaactg catgttcgtg tccagcggca ggctggccat
     2281 gtgcagcacc gcgtccgcta cgcgctgcac gtccatggtc ggctcgacct tgatctctcc
     2341 gttggcctga ggcacgccct tggtcatcct tgccgccatt tcggtcaacg cattgccgat
     2401 gtcgatctgg ccgcaggcga tgtcgtactt gcgtccgtcg agtgaagcgc acttggtgag
     2461 tccggtcacg gcgtgcttgg tagaggtgta aggcgatgag ttggggcgcg gcgcatgcgc
     2521 ggagatcgag ccgttgttga tgatccgtcc gccgcgcgga ttctggttct tcatcgtccg
     2581 gaacgcctcc tggatgcaga agaacatgcc ggagaggttg atgttcacta cgttgcgcca
     2641 ttgctcgacg gtcaggtcct cgagcgggat ccccggcgca ttaacaccgg cgttgttgaa
     2701 gagcaggtcc aggcggccaa aggcctcttt cgctttcgcg aataacgcct tcacggattg
     2761 cggatcgctc acgtcggtcg gcaccgccag tgcccgcgtg cccgctcccg attccttcac
     2821 ggtctgctcc agcgcatcct tgcgccgtcc cgcgagtgca acgctgtaac cgtccttcag
     2881 aagcgtgagc gcaacgctct tgccgatgcc gctgcctgcg cccgtaacga tggctatctt
     2941 gttgtgcgcg cccatttgcc tctcctcctc gaatagtgtc cgcaaaggcc gcatgctacc
     3001 gcatcgcgcc ggcaatttcc cgggggtagg agatcggcgg gggccgcaga cggtagaatg
     3061 cgcggcgaag gaggaagatc gtgatgccaa tcggaaggat tctgggagcg gtgctggcca
     3121 cgctgctgac tgcaatcggg ccggcggcgg cggacgcgct caaggacgac gccatagcaa
     3181 ggctggcggc gagccatgag cccgatgttc caatggctgt cggccgcctt gcggcgaagc
     3241 aggccggcct cggtgcggcg cggacgctgc tcgcccggcg ggggcgcgat gccggactcg
     3301 gccgcggctg gaatgcggct gcgccggagt ggcaggatgc cgaggcacac ttcagccgta
     3361 tcgtcgatgg catcatcgca cgcggcatcg aggatccgga ctggttgcat acgatctggg
     3421 ccgatcaggc agcccgggtg ctgaacgccg aggaagccga tgagattgcc acccatttcg
     3481 ctacgcctgt cggccgcgag cagcgcatcg tcatcgaagt caaggtggtg ggtgagctgc
     3541 tgctgaccag ctacacgttc acagaccgga tcagcgaccg ggttcccggg tcggagcgcg
     3601 aattcgctcg catgcaggcg atctgggcgg agcgcgaacc tttcaaggtg agaaacttcg
     3661 acggcgacgc cggcgccgcc cgcttcggct cgcgcaaccc gggcgtgaaa tacgtcagga
     3721 tgctcgcgat ccagggcgtc gacgcaatgc ttggacacat caacgcggtg tgcgccgagg
     3781 cggtgcttgc cgtgtcgcgg gctgccgcgc aagccgaccc cttcatcgaa tcctatcgac
     3841 ggcgcacggg gaactgagcc gaacgcccct atttgatctg atcggatgca gcaaaagcct
     3901 ggcgcagcgt cgccatggtg aatccgggct tgcgcgacgc atcgagtagc actttctccc
     3961 gtcgcgccat gagatcgatc gccgcaggca gcttgtcgag caggtcgcgc ggtatcgcga
     4021 tggcgccgtg gcggtcggca tggaccactt cgccgtcgga tacgaccagg ccggatacat
     4081 ccacctgtct gccgaaatcg acgacctgca cccaggcatg gctggggctc aacgatgcgg
     4141 ccaggcactg gaactgcggg tgcatcgcgt cgagatcgcg catcgagcca ttggttactg
     4201 cgccgacgca gcccaggccc cggtggatat gggtgttcac ttccccccag aacgcaccca
     4261 gcccgggctg actgtcgata tcctggacca cggcaaccgt cggctgctcc ggttgcgcta
     4321 gatactcgta gtaggcaagc tgatttttcc tgcgcagcgc cggttcgtaa ggcgtggaag
     4381 aacggatcgt caccgtcctg gcgaagccga cgatcggcgg cagttgcgga aacgcggcga
     4441 tcagcgtctg ccgggtgaag ccggtgatgg tgcgctgccc gcgcgcgatt tccagtgcgt
     4501 tggcgatggt aggcgtgtcg aagcggcgca gatgttcgaa caacgagcca gtgtccacgt
     4561 gaatcccccc tctttggttt atagtgagcc tagcggaatt gtcgcccggc ctggcgtttg
     4621 caggaccagg gaccgctatg ggtccgtagc ctcacggcga tacatgatcc caggattcgg
     4681 aggtctcatg ctgcataccg gtaactatct ggccgaccaa ctgctgattc cattcgtcct
     4741 gtggtttttc atcgtcgtgg ccgtcgtcgg gatcgccctg ggggtgtgct tgatcgccag
     4801 cagcgcccgg actttgcgct ttctcggcac cacgaaccgc tggatttcac tgcgaacccg
     4861 tttcaggccg atggaagcac cgcacgatat cggcaaggcc atatacgggc atcggcgctg
     4921 gttcggcgct gccttcgtgg tcggcggcgc ttttatgatg ttcatgctgc ttgcaagcgt
     4981 caagggcgcg gcggtcgtgt ccgcgctggg caggagtgct acccccgcag tcgtcgcctg
     5041 gatagttgaa agcctgaggt ggattctgct tgcaggcggc gcattggccg tcgtcaccgg
     5101 cgtaatgctg gtcttctcca gcgacgcatt gcgcgcattg gaagcgcgca cgaaccggtg
     5161 gtactcagcc agccaactcg gcaaaggcgc ggacaccatg tatctgacgc tcgaccaatg
     5221 ggtcgagacc tttccccgca ccgccggttg gatgctcggg ctcggtgccg cgatcgtatt
     5281 gatcgcctcg atcatcgtgt ggttcgggca gcgctaggca agcccgtcca gcggccgcag
     5341 gcgccgccgg ttcctagggc gcaaccgagg caggtgccgc cgtagggctt gaggtagact
     5401 gtaggcagtt catgccaggc gtaggcgccg attgccagct tcataataac gaccattcct
     5461 atagtcgagg attgaccggt gctggcgtat gctgtgtggc gaccgagact ttccttaccc
     5521 ccttgttgcc cgcatcatgg cgcgtgcata gatttcggcg cggtgcccgg aaaaaagtac
     5581 acccgggctg tccgggggag ccctgtacaa gtggcgtacc ggcggcaacg gcccagtgaa
     5641 gtcccggctc cccacccggg cctgcgtgtg aacgcaagcg cccgcggcgg caggccgccg
     5701 ttgttgcacg ccgccctggc agtgccgcgc ggaagaattg aaaaagtgca ctgaagaagg
     5761 agaatcagat agtgaatacg gaatcggcaa gcgacgaagt caccgacagc atcgcgcaca
     5821 tcgagatgca ccggcaggaa atggcgaagg aggccagcct ggcatcggcg tgcagcgagg
     5881 cgctgcgcaa gctgcacgaa ctcctcaagc acgagcagga agaattgcag aagaacggcc
     5941 cgcattccca acatacggcg aatatcgcgg cgctgactgg cgcaatcgaa aaggtgaaaa
     6001 gactgacggg cgtcaagaac gcggggccgc cttccaagga ccggcgtccg gagggacggc
     6061 gtcaaactcc catgcgcggc gcaaacccgg gcgcgacgag gaacaagggc cgcagaacga
     6121 tgggacggcg cggtgagcgt tgacaggttg tcagtgccac ggcgacagac caacacttac
     6181 cacggaggac gcggaggaaa ggcaagagaa gggaaaacct tcgagagtca aaacctgcca
     6241 tcggaagtca cttcgggacc agcgctactc ttaacgccca caagcttttt cctcgtttct
     6301 ggttttcctc cgtgtcctcc gtggtgagcg ctttcggctc tcagtacgac ttcggcaaac
     6361 ccagcacttt ctcggcgatg aagcagagga tgagctgagg gctgaccggc gcgagacggg
     6421 gaatcatcgc ttcgcgcagg tagcgctcga tgtgatactc cttggcatag cccatgccgc
     6481 catgcgtgag cagtgcctgt tcgcaggcgc tgaagcaggc ttccgcggcg agatacttgg
     6541 ccgcattcga ttccgcgccg cagggctcgt ctttgtcgta cagcgccgcc gccttgaaca
     6601 ccatcaggtt cgctgcctcg agttccatcc agcatcgcgc cagcggatgc tggatcgcct
     6661 ggttctgccc gatgggccgg tcgaaaacta cccgctcgtt ggcgtaggct accgcgcgcg
     6721 cgagtgccgc gtgcccgagg ccgaccgcct cggcggcgat caggatgcgt tcggggttga
     6781 gcccgtgcag gatgcagtcg aaacccttgc cgccctcgcc gatgcggtcc tgcaccggca
     6841 ccggcaactc ctcgatgaac agcatgttcg agtccaccgc gtttcggccc attttttcga
     6901 tttcccgcac ttcgacgtag cgccggtcga gatcggtgta gaacaacgac aggccgtcgg
     6961 tgggcttgcc gccgccctgc gcggctgcgg tgcgggcaag gatcagcatc ttgtccgcca
     7021 cctgcgcggt agagatccag atcttctggc cgcgcaggaa atagtgacta ccttcgcgcc
     7081 gcgccgacgt cgcaagcttc gcggtattga ggccggcatc cggctcggtc accgcgaaac
     7141 aggctttttg ctcgccgcga atcagcgccg gcagccagcg tttcttctgc tcgtctgttc
     7201 cgaacacgac gacgggattt agcccgaaga tgttcatgtg caacgcggac gcgccggaga
     7261 acccagcgcc ggactcggcc acggcctgca tcatcagcgc cgcctcggtg atccccagcc
     7321 cagcgccgcc gtactgctgc ggcatggcga tgccgagcca gccggattcg gcgacggcgc
     7381 ggtagaaatc ctcggggaag ccgccactgc gatcccgctc gagccagtac agcgcatcga
     7441 aacgcgcgca caacttcgcg atcgcctctt tgatggctag ttggtccggt gtgaaagaaa
     7501 aatccatggg caggggtgag gggcgagtgc taaccgaggc gtgtcacccc tttgtttgcc
     7561 aactcggtga tctccgtttc gttgtatccg gcttcgcgca gcacttcggc cgagtgttcg
     7621 cccagtcgcg gcgcaggccg ttgtccttcg ggctgcgatt cggaccagtc ggtgggcgtg
     7681 cgcatggtgc ggacaatccc ttcgctgggg tgcgcttcca aggtgaagaa tccggtcgcg
     7741 gcaaggtgcg gatcggcgac caggtcctcg accgaattca accgtgccac cgggatatcg
     7801 gccttctcca gcaattcgat ccattccgaa gtagttcgtg tcaggaacag ctccgcgaga
     7861 gtggcgtaga tcgcggagat atgccgggca cggctggctt gggtggcgaa gcgcgcgtcc
     7921 tgcatgattt ccggccggcc ggtggcatcg gtgaaagtct tccactgctt gtcgttgtaa
     7981 accagtacgc aaagatgccc gtccttggtg gggtaggggc ggcgggcgag cagccgggcg
     8041 taaccggcgt cgcccgcggg aggatcgaaa gtcagcccgg ccgagtgatc gcccagcacg
     8101 aaatgcgcaa ggctctcgaa catcggcact tccaccgact gtccgaggcc gctacggctg
     8161 cggtggaaca gtgccgcggt caccgcatag accgcgtgca atccggtgac ccggtcggcc
     8221 agattcaccg gcgtgtaatt cggggccttc gccccggtct tcgataccag ccacggaatg
     8281 gcggtgccgc cctggatcag gtcgtcgtaa gcggccttag ccgcgtacgg cccgcgctgc
     8341 gagtagccgt aggtgccgac gtagatgatc ctcgggttgg ccgcgcggac ttcctcgtag
     8401 gaaagcccga gccgtgccat cgcctgcggg cgcacgttgt agatcagcac gtcggtggcg
     8461 ggaatcaggc gcatcagcgc tttgcgcccc tcgggcttct tcaggtcgag cacgatgccg
     8521 cgcttgcccc ggttcaggtt caggaacatg tatcccatgc cgggggtttt catcggtgcc
     8581 ggatggcgca tgttgtcgcc ctcgtgcgct tcgaccttga tcacgtcggc gcccagctcg
     8641 gcgagaagct gggttgcaaa cggacccatg acgacggtgg tgagatcgag aacccttact
     8701 ccggataatg ggcctggcat tttttgactt gtgtttattc gggcggaatt cccgcgtcac
     8761 gcacgagttt accccagtac gcgatctgct cgcgcacgta gcggctgaac tcgctggtgt
     8821 tcatcggatc gatttcgaaa ccgatcgccg cgaatctttt cctgatctcc gaccgggaca
     8881 ggatttccag agtgtcgcgt tccagccgtg caacgatttc cttcggcgtc ccggccggcg
     8941 caaatatgcc gttccacgag gtgagatcga agccggtgag cgtcgccgcg atcggcggca
     9001 cctcgggcag cagcgtcgtg ggttttgccg tggtcaccgc cagtaccctg agcttgccgg
     9061 ccttaacgtg tggcatcgcg gtagtgaagt cggcgaccat gacctgcaag cgcccggcga
     9121 ccagatcgag aatcgcctgc gggctggaac tgtatccgac acggaccagg tcgagcccgg
     9181 cgagatggtt gatggtttca gccccgacca gcgacgcggt actcgccgtg ccgtaggaca
     9241 gcccgccggg atgggccttc gcatacgcga tgaactccgc ggtgttgttg gctggcagtt
     9301 ccgggttgac taccagcatg aacggcagcg tgccgacgcg cgcgatcggc tcgaagtctt
     9361 ttatcgggtc gtaggcaagc ttcttgtaga ggtgggggtt cgcgccatgg gtcgagttgg
     9421 tggtcatgaa cagcgtatag ccgtcggccg tgctcctggc cacgaacacc gcggcgatct
     9481 ggccattggc gcctgctcga ttctcgacga tcacgttctg gccgagccgc tgcgacaatt
     9541 cctgcgcgag aatgcggccg gcggaatcgg tcgcgctccc cgccgcgaac gcgatcacca
     9601 gtttgaccgg cttcgacgga taggcttccg ccagagcttg cggggatggt gccgagaaaa
     9661 cgatcgcgag cgtgaaagtg accagtctca ttatgtgatc aatgctcggc gctgaaaacc
     9721 aacagccaac gcagaggcgc agaggacgcg aaggccgcag agaaaagcag aattcggtgt
     9781 gtgtgaaaaa tcatgagtca agaaatgtcc gggagtgact gttgattacc aaaatcaggg
     9841 tcctcaacgc aatgccaact tccttgccac agggtaaaca gagtacacag agaaaaagcc
     9901 agactattct gctatcccga accgcgcatc ttccggccaa ccgtcggagg cgacgagctt
     9961 ctatttttcc cacatcttgg tttcctttgc gtcctttcct gttcttcctc tgcgtcctct
    10021 gcgtcaacgc ttttattgcc ggccatgggc cggctgctcg ccgtacggcg gcgagtagat
    10081 caccagcacc ttcaccggct cgtcgctgac caccgtgaac gtgtgccgca tgccgggcgg
    10141 aaagaagcaa cagtcccctg gaccgagctc gcacacttcg ccgccgacct cggcccgggc
    10201 gcggccttcg agcaggtagc acacctgctc tatgccggaa tgcgaatgcg gcaatgcacc
    10261 cttgcctttc tcgatgacgc cgagcacgac ttcgacctgg gctgcgccga cgttgccggc
    10321 gccgatcagg cggcgattga ctgtgccggt gtggttggcg ggatggtacg gcggcatatc
    10381 ctgcggacgg acgaagtacc gcgacgggat tggcaacgac atcgcttctc ctctcattcg
    10441 ggttcttgac aaaacaattt ccgaccgcac caggtgtttt tgcgactcac aggcaactac
    10501 gtacgaagct ggcctggtcc cgggccgcgc cgagcagcag gccaaggtcg ttgcccgcga
    10561 ggatcatgcg catcccgcgg ccgatgtaac gcttgagcaa atctttcgtg tagaccccgc
    10621 ccagtcccgg ccacttcccg tgcttccggc aggctccggt gacggcgtct atcgcggcca
    10681 ccacgcgttc gtgctccaac tggccgggaa tcccgatctc gagacaaaga tcgttcgtgc
    10741 cgatcagcag aacgtcgata ccggggaccg cggcaatctt ctcggcgttc tcgatcgcgc
    10801 gcggtgtttc cagcatcgcc accaccagag tcgcctggtt catttctgca acgacttcat
    10861 tgggcggcgc cgcggcaaaa ccgaactgcg gatagccgcc ggctatggac cgatgtccga
    10921 gcggcgtgaa tctgaacgca tccaccatgg cgcgcgcctg ttccggcgta tccacgtgcg
    10981 gcatcacgat gccgagcgcg ccgccgtcca ggcagcgggt ggcgagcgcg agttcgccgg
    11041 ccggcacgcg caccagaggc gcgatgccgg cgtcgagcgc cgccaccgag atcgaatacg
    11101 cggtctccac cgaggtcggc ccgtgctcca ggtcgatgaa cagccagtcg aagcccgcgg
    11161 ttttcatcac tctggcgatc tcgacgccgc gcaccgcccg cacgccgatc ccgatcgcga
    11221 tcgcaccctt cgatagcagc tcttttgccg gattcactat gcgttgcatt ttctctccta
    11281 ggtattgttg tgcccagtgc tcagtgcagt agatcaacac ccaacgcaga ggcgcagagg
    11341 acgcagaggg cgcagagaaa tacagaattc ggtgtgaacg aaattcatga ctcttgagat
    11401 gtgcaatggt gtctgatgat cgccaacgtc agagtcctta acgcaatcga aaacggatct
    11461 ttctgccaca gaacaacgga gagaaaaacc agacgatccc gccgtcttga tccgcgcatg
    11521 tccgagccaa ccgtatttct gctttttaca cacctggttt cctccgcgcc ctttcttgca
    11581 tttcctctgc gccctctgcg tcaacgctct tatcgccggc catcggccga ctgttccttc
    11641 atccttcatc cttccgcctt catccctgca cttcctccca tcttccgctt ttctcggagc
    11701 gaaacaacgc gtcgatcacg cgcatgctct gcaccgcgtc ttctacgccg tagggcagtg
    11761 atatttcgcc gcgaatcgcg cgcgaaaacg cctctccctg caaagtgtac tggtcgctct
//
LOCUS       DASQOP010000124         3902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_652567, whole genome shotgun sequence.
ACCESSION   DASQOP010000124 DASQOP010000000
VERSION     DASQOP010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652567"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="VE030_05000"
     CDS             <1..955
                     /locus_tag="VE030_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22810.1"
                     /translation="ELQRADATAEEKARAAAQARDAIEQASMEAAAAREGLVRLAEAV
                     RPNPRLEKAAEALRAGRTEEALALLRQMRAEAGGQDVRTLEKDGTRPLSDSEKGLDRG
                     IEERLAEGGRDLSSINARVDQDALDRVVNALQEANQRLEVQSRVIEVKRRMQEAMIPT
                     SQRSALTASQFDSSDNAPNATASPDTGNADIRGGAMFRQGAVTRRNDDEPARDGNMSG
                     SAAGNSEALALEGAATLRLDAQLKLEAIHRRDDAGDDEKADQGWFYSASQEQKSLLGF
                     EDVRARQGFDRENAMSVERVPIRQKHAVRDYFLNLHESEKK"
     gene            952..1947
                     /locus_tag="VE030_05005"
     CDS             952..1947
                     /locus_tag="VE030_05005"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZF22811.1"
                     /translation="MNTVVDRQIQAFRDQFQLVRDEISRVIVGNDEIISGVMTCLLAR
                     GHVLLEGIPGVGKTKLVQTMADVLHLKFSRIQFTPDLMPGDIIGTNIVREDEAGRKAF
                     EFQHGPIFANMVLADEINRATPKTQSALLEAMQENSVSAAGRTHALDQPFFVLATQNP
                     IEMEGTYPLPEAQMDRFFFKLKLDFPDVEQLHLIVDRTTGQKEPTVQRVLQQGEILEM
                     RETARAIPVARPVQEYAIRVTLATHPESSQAHEMSRKYVRFGSSPRGTQALILGAKVH
                     ALMNDRVYVSCEDVRAVALPALRHRILLNFEAEAGRIDTDTILDRILESVPEPKE"
     gene            2014..2901
                     /locus_tag="VE030_05010"
     CDS             2014..2901
                     /locus_tag="VE030_05010"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZF22812.1"
                     /translation="MAEESLLFSEEFLRQLEHLSMLTRRPVAGHLRGHHRSRRTGSGM
                     VFTDYRPYSPGDDTRNLDWGTYLRLDRLILRLFEEEADLPVYIFLDVSRSMDFGAPSK
                     FDFARKIAAALAYISLINHDRVSIVAFSDGVVTEMPARRGKNQVWRALHFLERIEAGG
                     GTSLQSAFQSFFGARRTRGLTVVISDFLDREGFERSFQVIRRFRHDVFAVHVMSPDEI
                     DPVLADEVLLVDSEQGGVARVHVTHDLVKAYHDTFLRYCAEIEAFCRSHGWGYLRTTT
                     AMPFEDLMLKALREQGLLR"
     gene            2898..>3902
                     /locus_tag="VE030_05015"
     CDS             2898..>3902
                     /locus_tag="VE030_05015"
                     /inference="COORDINATES: protein motif:HMM:NF019220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HZF22813.1"
                     /translation="MNLLSAAPLWAAIAALATVLVVTALYLLKPPPRRMLVPSSLIWD
                     RVLRQSHRSSDRLRWWLSLLLAAVIAVSIVAAVTRPQLTGAGEAADRLVLVLDNSPTL
                     ATRTTDGATRWSHALAKARARIAARGPTTQVMLVDSMRRIATPGFEDRDAALERLQQL
                     HVAYGGTPRVPDIAQAGDAQTVVVSDGVQITGVPKSAQLESVFEPVENAGITAFEIRP
                     LPADPGRYQAFVEVSNAGGADRSIEIAITGVGGRRIVRTVNVPAGGARAQLIDISILE
                     AGPVRASLTMPGDGLEADDVAYALLPMRRVVRVGLVSNGNPYLEKSLHAQPRVRLVAI
                     S"
BASE COUNT          718 a         1256 c         1302 g          626 t
ORIGIN      
        1 ggaattgcag cgcgctgacg cgaccgccga agagaaggcg cgagcagccg cgcaggcgcg
       61 cgatgcgatc gagcaggcca gcatggaagc tgcagctgcg cgcgagggcc tggtcaggct
      121 ggccgaggcc gtgaggccga atccgcgctt ggagaaggcc gcggaagccc ttcgcgccgg
      181 gcgcaccgaa gaggcgctgg cgttgctccg gcaaatgcgc gccgaggccg gcggccagga
      241 cgtacgtacg ctggagaagg acgggacccg gccgctgtca gacagtgaga aaggcctgga
      301 ccgcggcatc gaggaaaggc tcgcggaagg cggccgcgac ctgagcagca tcaacgccag
      361 ggtcgaccag gatgcactcg accgcgtggt gaacgcgctc caggaagcga accagcggct
      421 cgaagtacag agccgcgtaa tcgaagtcaa gcggcggatg caggaggcaa tgatcccgac
      481 gtcgcagcgg agcgcgctga ccgcgagcca gttcgattcc agcgacaacg cgcccaatgc
      541 gaccgcttcg ccggacaccg gcaacgcgga catccgcggc ggcgccatgt ttcgccaggg
      601 cgcggtgacg cgcagaaatg acgatgaacc cgcccgcgac ggtaacatga gcggcagtgc
      661 cgccggcaac tccgaagccc tggcgctgga gggtgcagcg accctgcgcc tggatgcgca
      721 actgaagctc gaagccatcc atcgccgcga cgatgccggg gacgacgaaa aggccgacca
      781 gggctggttt tattccgcca gccaggagca aaaatcgctg ctcggcttcg aggacgtgcg
      841 cgcccgccag ggcttcgacc gcgagaacgc gatgagcgtc gagcgcgtgc cgatccgcca
      901 gaaacatgcc gtaagggatt atttcctgaa cctgcacgaa agtgagaaga aatgaatacc
      961 gtcgtcgacc gccagatcca ggccttccgc gatcagttcc agcttgtccg cgacgagatt
     1021 tcgcgggtca tcgtcggtaa cgacgagatc atttccgggg tgatgacttg cctgcttgcg
     1081 cgcggccacg tgctgctgga gggaatccca ggcgtgggca aaaccaagct ggtgcagacc
     1141 atggcggacg tgctgcacct gaagttctcg cgcatccagt tcacccccga cctgatgccg
     1201 ggcgatatca tcggcacgaa catcgtccgc gaagacgagg ccggccgcaa agccttcgaa
     1261 ttccagcacg gcccgatctt tgccaacatg gtgctggccg acgaaatcaa ccgcgccacg
     1321 cccaagaccc aatcggcatt gctggaggcg atgcaggaga acagcgtttc cgcagccggc
     1381 cgcacccacg cgctggacca gcccttcttc gtgctggcaa cccagaaccc gatcgagatg
     1441 gaaggcacgt atccgctgcc cgaggcgcag atggatcgct ttttcttcaa actcaagctc
     1501 gatttcccgg acgtggagca gctgcatctg atcgtcgacc gcactaccgg gcagaaggaa
     1561 ccgacggtac agcgggtgct gcaacagggc gagatcctgg agatgcgcga aacggcacgc
     1621 gctattccgg tggcgcggcc ggtgcaagag tacgcgatca gggtgacgct ggcgacccat
     1681 ccggaatcct cgcaggcgca cgaaatgagc aggaagtatg tgcgtttcgg ttccagcccg
     1741 cgcggaaccc aggcgctgat cctgggtgcg aaagtacacg cattgatgaa cgaccgcgtc
     1801 tacgtgtcct gcgaggacgt gcgcgccgtg gcattgccgg cgctgcggca ccggatcctg
     1861 ctgaacttcg aggccgaagc cggccgcatc gataccgaca cgatcctcga caggatcctc
     1921 gagtcggtgc cggaaccgaa ggaatgaccg ccgactgagg atatccaccg gccccatggc
     1981 ggctgccatg cggccccact gaggcatttc aatttggccg aggaaagcct gctgttcagc
     2041 gaagaatttc ttcgccagct cgagcatctc agcatgctta cccgccgtcc cgtggcgggc
     2101 cacctgcgcg gccatcatcg gtcccgccgc accggctcgg ggatggtgtt caccgactat
     2161 cggccgtact cgccgggcga tgacacgcgc aatctcgatt ggggcacgta cctgcggctg
     2221 gaccggctga tcctgaggct gttcgaggaa gaggcggacc tgccggtcta cattttcctc
     2281 gacgtcagcc gttcgatgga tttcggcgcc ccgtccaagt tcgacttcgc ccgcaagatc
     2341 gccgccgcgc tggcctacat aagcctgatc aaccacgacc gggtcagcat cgtcgccttt
     2401 tccgacgggg tcgtcacgga gatgccggcg cggcgcggca agaaccaggt gtggcgcgcg
     2461 ctgcattttc tcgagcgtat cgaggccggc ggcggcacca gcttgcagtc ggcgttccag
     2521 agtttcttcg gtgcccggcg cacccgcggg ctgacggtgg tgatctccga cttcctcgac
     2581 cgcgagggtt tcgagcggag cttccaggtg atacgccgtt tccgccacga tgtattcgcg
     2641 gtgcacgtga tgagccccga cgaaatcgac ccggtgctcg cggatgaagt gctgctggtg
     2701 gactcggagc aaggcggcgt cgcgcgggtg cacgtgacgc acgacctggt gaaggcctat
     2761 cacgacacgt tcctgcgcta ttgcgccgag atcgaagcct tctgccgcag tcacggctgg
     2821 ggctatctgc gcaccaccac ggccatgccg ttcgaagacc tgatgctcaa ggcgttgcgc
     2881 gagcaaggct tgttgcggtg aacctgcttt cggctgcgcc tctgtgggcc gcgatcgcgg
     2941 cgcttgccac ggtgctggtc gtaaccgcgc tgtatctgct gaagccgcct ccacgcagaa
     3001 tgctggtgcc ctccagcctt atctgggacc gggtgttgcg ccaaagccat cgcagcagcg
     3061 accgcttgcg ctggtggctg tcgttgctgc tggccgcggt catcgccgta agcatcgtcg
     3121 ccgccgtgac cagaccgcag ttgaccggcg cgggagaggc cgctgacagg cttgtcctcg
     3181 tgctcgacaa ctcgccgacg ctggccaccc gcacaaccga cggtgcgacc cgctggagtc
     3241 acgcgctggc caaggcgcgc gcgcgaatcg ccgcgcgcgg cccgactacc caggtgatgc
     3301 tggtcgattc catgcggcgc atcgccactc ccggtttcga agaccgcgac gcggcgcttg
     3361 agcggctgca gcaacttcat gtggcttacg gcggcacgcc gcgggtgccg gacattgcgc
     3421 aggccggcga tgcgcaaacc gtggtggtca gcgacggggt gcagatcacc ggggtgccga
     3481 agtcagccca actcgagtcg gtgttcgagc cggtggaaaa cgccggcatt actgcgttcg
     3541 aaatccggcc gctgcctgca gacccggggc gctaccaggc cttcgtcgag gtcagcaacg
     3601 ccggcggcgc ggacagaagc atcgagatcg cgattaccgg tgtcggcggc cggcgcattg
     3661 tcaggaccgt gaacgtgccc gcaggcggcg cgcgcgcgca actgatcgac atttccattc
     3721 tggaggccgg tccggtgcgc gcctcgctca ccatgcccgg ggacggcctg gaggccgatg
     3781 acgtcgctta cgcactgctg ccgatgcggc gcgtggtgcg agtgggcctg gtgagcaatg
     3841 gtaatcccta cctcgagaaa tccctgcatg cgcagccgcg agtgcggctc gtggcgattt
     3901 cg
//
LOCUS       DASQOP010000125         9017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_454763, whole genome shotgun sequence.
ACCESSION   DASQOP010000125 DASQOP010000000
VERSION     DASQOP010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9017
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454763"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..177)
                     /locus_tag="VE030_05020"
     CDS             complement(<1..177)
                     /locus_tag="VE030_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZF22814.1"
                     /translation="MKVRVKWIEHVAFLGETESGHALVMDGAPEGGGRNLGPRPMETM
                     LAGTGGCTAYDVVTI"
     gene            444..1040
                     /gene="petA"
                     /locus_tag="VE030_05025"
     CDS             444..1040
                     /gene="petA"
                     /locus_tag="VE030_05025"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HZF22815.1"
                     /translation="MADSSVDRRKRHFLVAATSVAGGVASVAVAVPFVASMNPSARAR
                     AAGAPVEVDLSKVEPGMMVTVEWRGQPVWVVHRTREMIDSLPKLDEVVADPKSSMPMQ
                     PDYAKNEVRSIKPEYLVLVGICTHLGCSPSQKLAPGPESDLGKDWPGGFFCPCHGSKF
                     DLAGRVYKGVPAPANLRVPPYTYLSDTRLIIGEDSKGA"
     gene            1045..2316
                     /locus_tag="VE030_05030"
     CDS             1045..2316
                     /locus_tag="VE030_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013211301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HZF22816.1"
                     /translation="MGKMTALLNWIDARFPLTATWRTHLSEYYAPKNFNFWYYFGSLA
                     MLVLVMQIVTGIFLTMHYKPDAAQAFASVEYIMRDVDWGWAIRYMHSTGASLFFVVVY
                     LHMFRGLIYGSYRRPRELLWLLGVLIYLCLMAEAFFGYLLPWGQMSYWGAQVIVNLFA
                     AIPFVGPDLAVWIRGDYVISDATLNRFFAFHVIAIPLVLLGLVFAHLVALHEVGSNNP
                     DGIEIKNKKDPKTGIPLDGIPFFPYYVVKDIVGVVVFLMVFSVIVFFAPDMGGYFLEH
                     NNFVPADSLKTPAHIAPVWYFTPFYSILRAVPPLFGSQFPGVLAMGLAVLIFFFLPWL
                     DRGAVKSVRYRGTVYKTALALLVISFVVLGYLGTVPTNVWGQFGGWLGGAERATVVAR
                     IFTIIYFLFFLLMPWYTKIDKTKPEPARVTW"
     gene            2310..3056
                     /locus_tag="VE030_05035"
     CDS             2310..3056
                     /locus_tag="VE030_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HZF22817.1"
                     /translation="MVKRFILALLFAPAAAHAAGGDIRLDRASIDSGDVLSIQRGARV
                     FVNYCLNCHSAAAMRYNRLTDLGLTERQIKDNLLFAGEKVGDTMQIAAGKKDQQEWFG
                     VAPPDLSVIARSRGVDWLYTYMRSFYRDPARVTGWNNLAFPNVGMPHVLWQLQGTQVL
                     KSEIEEHGGREVEHRKLVLESPGILSPLEYDKLARDLVNYLSYMGEPAQARRVQIGIV
                     VLLFLSLLLVLSWLLKTEYWKDVHRPSTSR"
     gene            3063..3662
                     /locus_tag="VE030_05040"
     CDS             3063..3662
                     /locus_tag="VE030_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HZF22818.1"
                     /translation="MMTLYSGTTCPFSQRCRIVLYEKGMDFQIIDVDLYNKPEDLAVM
                     NPYNRTPVLVERDLILYESNIINEYIDDRFPHPQLMPADPVMRARARLFLFRFENELF
                     SNIDAIEGGTQKQAEKARQIIRDNLTQIAPVFVKQKYMLGEEFSMLDVAIAPLLWRLD
                     FYGIQLPKQAAPLMKYAERLFSRPAFIEALTASEKVMRK"
     gene            3659..4084
                     /locus_tag="VE030_05045"
     CDS             3659..4084
                     /locus_tag="VE030_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HZF22819.1"
                     /translation="MKETATTPYLIRAIYEWCCDNGLTPYVAVKVDETTKVPTEYVKN
                     GEIVLNVSPDATRNLKIGNDRIQFSARFGGTSREISVPITAVAGIFAKENGHGLAFQT
                     SPSLPAPVVAPPKAHPSSAPDDHRPKPPAGRRPRLQVIK"
     gene            4136..4211
                     /locus_tag="VE030_05050"
     tRNA            4136..4211
                     /locus_tag="VE030_05050"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4169..4171,aa:Thr,seq:tgt)
     gene            4296..4652
                     /locus_tag="VE030_05055"
     CDS             4296..4652
                     /locus_tag="VE030_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22820.1"
                     /translation="MIIAVTAFAQSAETPILTIEQAQQRAQYAREQMTAAERKVRAAE
                     KKEQAAYKDLEAAQKRMEDAQRAYEEAKVRAEQATQASRALQAEFDQARAKHQQEYQN
                     LKRAHDAAEAARKAPR"
     gene            complement(4649..5197)
                     /locus_tag="VE030_05060"
     CDS             complement(4649..5197)
                     /locus_tag="VE030_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZF22821.1"
                     /translation="MNTRPATDNRLLVVDDDAVFAAVLARALARRGFEVESAAGVGEA
                     VEKAGRRGPQFAVVDLKMPDGSGLALVEQLKRLNPAMRIVVLTGFASVATAVEAIKLG
                     AVHYLPKPADADDIVAAFDRDAGDAAVAVKHRPLSVERLEWEHIHKVLAENGGNISGA
                     ARALGMHRRTLQRKLRKKPGRN"
     gene            complement(5194..6453)
                     /locus_tag="VE030_05065"
     CDS             complement(5194..6453)
                     /locus_tag="VE030_05065"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZF22822.1"
                     /translation="MNAPANAHSNLRRLFFLRSITVGGQVLVIAAAVLMFDTPLPLGA
                     MGAIIVLQTAANGRTWMRLRKAAPVSEAELFLELLADVGALTALLYLSGGSTNPFVSL
                     YLLPLIIAATALTARYAWAMAGLTIACYSALMFFYLPLGHGHEMHSNAFNLHVLGMWA
                     NFLVSAVLIASFVTTMSASIRGRDRELAIARARALRDEQVVALGTFAAGAAHELGTPL
                     STIAVLTKELERQYAADPGLREDLQLLRTQVDNCKRIITSLTTAAGLARAGQAKRQSV
                     EEFLAGVAEKWALIRPQVRLDLRCGGAGAAPQIVGEETVRQTLINILNNAADASPHAV
                     EIEGTWNAAELTIEVRDRGPGITDDIAAKAGRAFFSTKAAGRGIGLFLANATIERLGG
                     SVALFNRDGGGGCTRVTIPLTRLAADA"
     gene            6603..7550
                     /locus_tag="VE030_05070"
     CDS             6603..7550
                     /locus_tag="VE030_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22823.1"
                     /translation="MLATIRWSSFAILAGLLPLPAWAGCGSAVCSINTNWDAQGAWTE
                     PGARFDLRYEFIDQDQPMSGDSRVGVGEIPRHHDEVRTINRNWIATLDYAFSQRLGVS
                     VSLPVVDRSHDHIHNHGGGQFPENWNFTEAGDARVLGRYHVGESAEAGMGLLLGVKLP
                     TGSFKVVNDDGERAERTLQPGTGTTDALLGAYYRAPGSGHGAWFAQVLWQAALGERED
                     FRPGDRSSVDLGYRHQLNERFAALLQLNLLYRDRDGGAQAEPEDSGGRFAFIAPGLSY
                     NLGRDTQLYGFVQVPVYQYVNGVQLTADWSLVAGFATRF"
     gene            7734..8894
                     /gene="sucC"
                     /locus_tag="VE030_05075"
     CDS             7734..8894
                     /gene="sucC"
                     /locus_tag="VE030_05075"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HZF22824.1"
                     /translation="MKIHEYQAKEILRKYGVPTPRGTPCFSVEEAVSAARKLGGKVWV
                     VKAQTHAGGRGKGGGVKLAKSIDEVRDWAGKILGMQLITHQTGPKGQKVRRLLIEEGA
                     DIDRELYIGMVVDRGTQTVCVMASSQGGMDIEEVAAHTPEKIHKVFVDPERGLLDAEA
                     DDLARRIGIPAASVAKARTVLKNLYRAFWDTDASLAEINPMILTGGGDVVALDAKMNF
                     DDNALFRHEDIVAMRDLDEEDPAEVEASRHGLSYISLDGNIGCLVNGAGLAMATMDTI
                     KLFGGEPANFLDVGGGATTEKVTEAFKLMLRNPNLSAILVNIFGGIMKCDVIATGVVE
                     AAHQVKLGVPLVVRMKGTNEDLGRKILADSGLPIISADNMAEAAQKVVAAAK"
BASE COUNT         1656 a         2855 c         2839 g         1667 t
ORIGIN      
        1 gatagtcacg acgtcgtaag ccgtacaacc gccggtgccc gccagcatcg tttccatcgg
       61 ccgcggcccg agattgcggc cgccgccctc cggcgcgcca tccattacca gcgcatgccc
      121 gctctcggtc tcacccagga acgcaacgtg ctcgatccat ttaaccctga ctttcatcga
      181 cgtcctcgaa gccggtttgc agcccgtatg cgtcctcatc cactgccacg gagcgcgagg
      241 cgcagatggt accagccgag gccgacactg cgcgcaggca aagttgcacg aacagatttt
      301 ccggaggcct ggcagcaaat tctgtccaaa gtttgctggc ggcgatttct ctagaattta
      361 catggactta agcaacgtgg ggtgtaattt gcccaggcac ctactgtaaa atgcaacgct
      421 tttgaaacct gacggaccgg aacatggctg acagcagcgt cgatcgccgc aaacggcatt
      481 ttcttgtagc cgccaccagc gttgccggcg gcgtggcgag tgtcgccgtc gccgtgccct
      541 tcgtggcgag catgaacccg agcgcgcggg cgcgcgctgc cggcgcgccg gtcgaggtgg
      601 atctgtccaa ggtcgagcct gggatgatgg ttacggtgga atggcgcggc cagccggtgt
      661 gggtcgtcca tcgcactagg gaaatgatcg acagcctgcc caagctcgac gaggtggtgg
      721 ccgatcccaa atcctcaatg ccgatgcagc cggattatgc aaagaacgaa gtccgctcga
      781 tcaaacccga atacctcgtg ctggtcggca tctgcactca cctcggctgt tcgccgtctc
      841 agaaactcgc acccggaccc gaaagcgatc tgggcaagga ctggccggga ggtttctttt
      901 gtccctgtca cggttccaag ttcgatctcg ccggccgcgt ctacaagggc gtaccggccc
      961 ccgcgaatct cagggttccc ccgtatacct acctgagcga cacgcgtctg atcattggtg
     1021 aagacagcaa aggagcatga cgcgatgggc aagatgacgg cgctgctcaa ctggatcgac
     1081 gcgcgctttc cgctgacggc gacctggagg acgcaccttt ccgagtatta cgcgccgaag
     1141 aacttcaact tctggtacta cttcggctcg ctggcaatgc tggtgctggt gatgcagatc
     1201 gtcaccggca ttttcctcac catgcactac aagcccgacg ccgcgcaggc tttcgcttcg
     1261 gtcgagtaca tcatgcgcga cgtcgactgg ggatgggcga tacgctacat gcattccacc
     1321 ggcgcgtcct tgttcttcgt ggtggtgtac ctgcacatgt ttcgcggcct gatctacggt
     1381 tcctaccgca ggccgcgcga gttgctgtgg ctgctgggcg tgctgatcta cctgtgcctg
     1441 atggcggagg ccttctttgg ctacctgctg ccgtggggcc agatgtccta ctggggcgcg
     1501 caggtgatcg tcaatctgtt cgcggctatc cccttcgttg gtcccgacct ggcggtgtgg
     1561 atccgcggcg actacgtgat ttccgacgcg acgctgaatc gttttttcgc ttttcacgtg
     1621 atcgcgatcc cactggtgct gctgggcttg gtctttgcgc acttggtggc gctgcacgaa
     1681 gtcggctcca acaatccgga cggcatcgag atcaagaaca agaaggatcc gaagaccggc
     1741 attccgctcg acggcatccc cttcttcccc tattacgtgg tgaaagacat cgtcggcgtg
     1801 gtggtgttcc tgatggtgtt cagcgtcatc gtgttctttg cgccggatat gggcggctac
     1861 ttcctcgagc acaacaattt cgtgcctgcc gattcgctca agaccccggc ccacatcgcc
     1921 ccggtgtggt atttcacgcc gttctattcg atcctgcgcg ctgtgccgcc gttgtttggc
     1981 tcgcagtttc cgggcgtgct ggcaatgggt cttgcggtgt tgatcttctt cttcctgccg
     2041 tggctggatc gcggcgccgt caagtccgtc cgctaccgcg ggacggtcta caagaccgcg
     2101 ctcgcgctgt tggtgatctc cttcgtcgtg ctgggctatc tcggcaccgt gccgaccaat
     2161 gtctggggcc agttcggagg ctggctgggc ggcgcggaac gcgcgaccgt ggtggcgcgc
     2221 atcttcacga tcatctattt cctgttcttc ctgctgatgc cctggtatac caagatcgac
     2281 aagaccaagc cggagcctgc gagggtgaca tggtgaaaag attcattctc gcgttgctgt
     2341 tcgcgccggc tgcggcccat gccgccggcg gcgatatccg gctcgatcgc gccagcatcg
     2401 attccggcga cgtgctgtcg atccagcgcg gcgcaagggt gttcgtcaat tactgcctga
     2461 actgtcactc cgcggcggcg atgcgctaca accgcctgac ggatcttggg ctgaccgaac
     2521 ggcagatcaa ggacaacctg ctgttcgcgg gcgaaaaggt cggtgatacg atgcaaatcg
     2581 ccgccggcaa gaaagaccag caggagtggt tcggcgtcgc gccgccggat ctttccgtga
     2641 tcgcccgctc gcgcggcgtg gactggctct acacctacat gcgcagtttc tatcgcgacc
     2701 ccgcgcgcgt gaccggctgg aacaacctgg cgtttccgaa cgtcggaatg ccgcacgtgc
     2761 tctggcagct gcagggcacc caggtgctga aatccgagat cgaggaacac ggcgggcgcg
     2821 aggtcgagca caggaagctc gtgctggaat ccccgggaat cctgtcgccg ctggagtacg
     2881 acaagctcgc gcgcgatctg gtgaactacc tttcctacat gggcgaaccg gcccaggccc
     2941 ggcgcgtcca gatcggcatc gtcgtactgc tcttcctttc gctcctgctt gtgctcagtt
     3001 ggctgctcaa gacggagtac tggaaagacg tgcaccgacc atcaacatct aggtaacggc
     3061 ccatgatgac cctttattcg ggcaccacct gcccgttcag ccaacgctgc cgcatcgtcc
     3121 tctacgaaaa ggggatggat tttcagatca tcgacgtgga cctctacaac aagccggagg
     3181 atctcgcggt gatgaacccg tacaaccgca ccccggtgct ggtggaacgc gacctgatac
     3241 tttacgagtc gaacatcatc aacgagtaca tcgacgaccg cttcccgcat ccgcaactga
     3301 tgccggccga tcccgtgatg cgcgcgcgcg cgcggctgtt cctgttccgt ttcgagaacg
     3361 agctgttctc caacatcgat gcgatcgagg gcggcaccca gaagcaggcc gaaaaggcgc
     3421 gccagatcat ccgcgacaat ctcacgcaga tcgcaccggt gttcgtcaag cagaagtaca
     3481 tgctcggcga agaattctcg atgctggacg tggcgatcgc gccgctgctg tggcgactgg
     3541 atttctacgg catccagctg cccaagcagg ccgcacccct gatgaagtac gccgagcggc
     3601 tgttcagccg tcccgctttc atcgaggcgc tcacggcttc cgaaaaggtg atgcgcaagt
     3661 gaaggaaacc gcaaccaccc cctacctgat ccgcgcgatc tacgagtggt gctgcgacaa
     3721 cgggctgact ccgtacgttg cggtcaaggt ggacgaaacc accaaggtac cgacggagta
     3781 cgtcaagaac ggcgaaatcg tgctcaacgt aagtcccgac gccacccgca atctgaagat
     3841 cggcaacgac cggatccagt tctcggcgcg tttcggcggt acttcgcgcg agatctcggt
     3901 gccgatcacc gcggtggcag gaatcttcgc caaggaaaac gggcatggcc tggcgttcca
     3961 gacttcaccg tcgctgcccg ccccggtggt cgcgccgccg aaggcccacc ccagttctgc
     4021 tccggacgac caccgtccca agccgccggc gggccgccgg ccgcggctgc aggtaatcaa
     4081 gtaactgact ggcgcggttt cgtggccgca acgctgggcc tataatgcgg cgcaagccgg
     4141 catagctcag ttggcagagc agttgatttg taatcaaaag gtcgcgggtt cgattcctgc
     4201 tgccggcacc agcgataacc ctgccttcct gaattagcgt cgaaacagcg ggcgccggag
     4261 aaaacatgcc gagacttttt ccagcaggcg cattgatgat catcgcggtc actgccttcg
     4321 cgcagtcggc cgaaacgccc atattgacca tcgagcaggc acagcagcgt gcccagtacg
     4381 cccgcgagca gatgacagcc gccgaacgca aggtgcgcgc cgcagagaaa aaggaacagg
     4441 ccgcttacaa ggacctggag gccgcgcaaa aacgcatgga ggatgcgcag cgcgcctacg
     4501 aggaggccaa ggttcgcgcc gagcaggcga cccaggcgtc gcgcgcactg caggccgagt
     4561 ttgatcaggc gcgcgccaag catcaacagg aataccagaa tctgaaacgc gcccacgatg
     4621 ccgccgaagc ggcccgcaag gcgccccgct agttccggcc cggtttcttt cgcagctttc
     4681 tctgcagtgt gcgccggtgc atgccgagcg cgcgcgcggc gccggaaata ttgccgccgt
     4741 tttcggcgag cactttgtgg atgtgctccc actccagccg ctcgaccgac agcggcctgt
     4801 gcttgaccgc gaccgcggcg tcacccgcgt cgcggtcgaa agccgccaca atgtcgtcgg
     4861 cgtctgctgg tttgggcagg tagtggaccg cgcccagctt gattgcctcg accgcggtgg
     4921 caacgctggc gaagccggtg aggacaacga tgcgcatcgc cggattgaga cgcttgagct
     4981 gctccaccag tgcgagtccg gagccatccg gcatcttcag gtccaccacc gcaaactggg
     5041 gaccgcggcg ccccgccttc tcgactgcct cgccaacacc ggccgcggac tcgacctcga
     5101 atccgcgccg ggcgagtgca cgcgccagca ctgcggcaaa caccgcgtca tcgtccacca
     5161 ccagcaggcg gttgtcggtt gccggcctcg tgttcatgcg tcggcggcca gccgcgtcag
     5221 tggaatcgtc acccgcgtgc agccgccgcc gccgtcgcgg ttgaacagcg ccacgctgcc
     5281 acccaggcgc tcgatggtgg cattggccag gaacagtccg attccgcgtc ccgccgcctt
     5341 ggtgctgaag aaagcgcgtc ccgccttcgc ggcgatgtca tcggtaatgc ctggaccgcg
     5401 atcgcgcact tcgatggtga gttcggcagc gttccaggtg ccctcgatct ccaccgcgtg
     5461 cggggaggcg tccgcggcgt tgttgagaat gttgatcagg gtctgacgca cggtttcctc
     5521 gccgacgatc tgcggcgcgg cgcccgcgcc gccgcagcgc aggtcgagcc gcacctgcgg
     5581 gcggatcagg gcccactttt ccgccacgcc ggccaggaac tcctcgacgc tctgccgctt
     5641 ggcctgtccg gcacgcgcca gcccggccgc ggtggtcagg ctggtgatga tgcgcttgca
     5701 gttgtcaact tgtgtgcgta acagttgcag gtcctcgcgc aggcccgggt cggcggcgta
     5761 ctggcgctcc agttcctttg tgagcacggc tatggtcgag agcggcgtgc ccagttcgtg
     5821 cgccgcgccc gcggcaaacg tgcccagcgc gaccacctgc tcatcgcgca gcgcccgcgc
     5881 ccgcgcgatg gcgagttcgc ggtcgcgccc gcggatggac gccgacatgg tggtgacgaa
     5941 gctcgcgatc agcaccgcgc tcaccaggaa gttcgcccac attcccagca cgtgcaggtt
     6001 gaatgcgttc gaatgcatct cgtggccatg acccagcgga aggtagaaaa acatcagcgc
     6061 cgaatagcag gcgattgtca acccggccat ggcccaggcg taacgcgccg tcagcgcagt
     6121 cgcggcgatg atgagcggca acaggtacag agacacaaag gggttggtgg agccgccact
     6181 cagatagagc agcgcggtca gtgccccgac atcggcgagc aactcgagga acagttccgc
     6241 ttcgcttacc ggcgcggctt tgcgcagccg catccaggtg cggccgttgg cggcggtctg
     6301 gagcacgatg atcgcgccca tcgcacccag cggcagcggc gtgtcgaaca tcaggactgc
     6361 ggcggcgatc accagcacct gtccgcctac ggtgatgctg cgcaggaaaa acaggcgccg
     6421 cagattggaa tgggcgttgg ctggagcatt catggcggcg accggacctg ggggaaagtg
     6481 gagattttat tcttttgcgg accatttgcc gggctgcggc aatttgtcgc gcggcaacgt
     6541 gtcgcattcc gctcatccgg tcgcgcgtct agcattgcgc ttttcgatcg gaagtcacgg
     6601 tgatgctcgc aacaattcgg tggtcatcgt ttgcgatcct ggccggactg ttgccgctgc
     6661 cggcgtgggc cgggtgcggc tcggcggtct gttcgatcaa taccaactgg gacgcgcagg
     6721 gcgcatggac cgagccgggc gcgcgcttcg atctgcgtta cgagttcatc gatcaggatc
     6781 agccgatgtc cggagattcg cgcgtcggcg tgggcgagat cccccgccac catgacgagg
     6841 tccgcaccat caaccgcaac tggatcgcca cgctcgatta cgcgttcagc cagcggctcg
     6901 gcgtctcggt cagcctgccg gtggtggacc gcagccacga tcacatccac aaccacggtg
     6961 gaggccagtt tcccgagaac tggaacttca cggaagccgg tgatgcccgg gtactcgggc
     7021 gctaccacgt tggcgagtcc gccgaggcgg gcatgggact gctgctcggc gtgaagctgc
     7081 cgaccggtag tttcaaagtg gtgaacgacg atggcgagcg cgctgaacgc actctgcagc
     7141 ccggcaccgg caccaccgac gcgctgctag gcgcctacta ccgtgcgccc ggctccggtc
     7201 acggcgcctg gttcgcgcag gtgctgtggc aggcggcgct gggcgagcgc gaggactttc
     7261 ggccgggaga tcgctctagc gtggacctgg gctatcgcca ccagctgaac gagcggtttg
     7321 ccgcgctgtt gcagttgaat ctgctctatc gcgaccgcga cggcggcgcg caagccgaac
     7381 cggaggacag cggcggccgc tttgccttca tcgccccggg actcagctac aaccttggcc
     7441 gcgacacgca gctctatggt ttcgttcagg tgccggtcta ccagtacgtc aacggcgtgc
     7501 agttgaccgc cgattggtcg ctcgtggcgg ggttcgcgac ccgcttctga gcgttgccgc
     7561 gttgcagcgg cggcgcgggc ctttgcggta tacttcccgc gctttcccgc gcggtcggct
     7621 tgttctgcga ccttccagct ggcgatccct tgttccgttc cggcgtgcgc cgcatgcacg
     7681 cgggaattcg gcgtgccagc ccgccgcggg tccgattcag gcaagggccg gcgatgaaaa
     7741 tccacgaata ccaggcgaaa gaaatcctca ggaagtacgg tgtccctacg ccccgcggca
     7801 cgccctgctt cagcgtggaa gaagcggtga gcgctgcaag gaaactcggc ggcaaggtat
     7861 gggtggtgaa ggcacaaacc cacgccggcg gccgcggcaa gggcggcggg gtgaaactcg
     7921 cgaaatccat cgacgaggtg cgcgactggg ctggaaaaat cctcggcatg caactgataa
     7981 cccatcagac cggacctaag ggccagaagg tgcggcggct gctgatcgag gaaggcgcgg
     8041 acatcgacag ggagctttac atcggcatgg tggtggaccg cggcacgcag accgtgtgcg
     8101 tcatggcgtc ctcgcaaggc gggatggaca tcgaggaagt cgccgcgcac acgcccgaga
     8161 agatccacaa ggtgttcgtc gatcccgagc ggggcctgct tgatgccgaa gccgacgatc
     8221 tggcccgcag gattggcatt cccgctgcca gcgtggccaa ggcgcgtact gtgctcaaga
     8281 acctctatcg ggcattctgg gacaccgatg catcgctcgc cgaaatcaac ccgatgatcc
     8341 tgaccggcgg cggcgatgtg gtcgcgctcg acgccaagat gaacttcgac gacaacgcac
     8401 tcttccggca cgaggatatc gtcgccatgc gtgacctcga cgaggaagac ccggccgagg
     8461 tggaagcgtc caggcacggg ctgtcttata tatcgctcga cggcaatatc ggctgcttgg
     8521 tgaacggcgc cggcttggcg atggccacca tggacacaat aaagttattc ggtggcgagc
     8581 cggccaactt cctcgatgtc ggcggcggcg ccacgacgga gaaggtgacc gaagccttca
     8641 agctgatgct gcgcaacccc aacctcagcg cgattctggt caacatcttc ggcggcatca
     8701 tgaaatgcga cgtgatcgcc accggggtgg tcgaggcggc ccatcaggtg aagctcgggg
     8761 tgccgctggt ggtgcgcatg aagggtacca acgaggacct cggcagaaag attcttgcgg
     8821 attccggatt gccgatcatt tccgccgaca acatggcgga agcagcacag aaggtggtcg
     8881 cggctgctaa gtgacaagct gaccacagag acacagagaa aagcttgaga acgaatcaac
     8941 tcaccgccaa gacgccaagt acgccaagag cagcattgag tacgccaaat ttctttcatt
     9001 atttttcgcg tttcgaa
//
LOCUS       DASQOP010000126         7971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2471192, whole genome shotgun sequence.
ACCESSION   DASQOP010000126 DASQOP010000000
VERSION     DASQOP010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7971
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2471192"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1114)
                     /locus_tag="VE030_05080"
     CDS             complement(<1..1114)
                     /locus_tag="VE030_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006712609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZF22825.1"
                     /translation="MISPSDGQAFASIARGARSDVDAAVVAARRAFEEGAWGRMTAVE
                     RGRLMAKLGEAILAHHEELAQLEARDCGKPMKQARADITAAARYFEFYGAAADKFHGE
                     TIPFLDGYMVTVLREPKGVTGHIIPWNYPAQMFGRSLAPALAMGNAVVMKPAEEACVV
                     PLRLAQLAQDVGFPEGAINVVTGIGEEAGAALASHAGIDFLSFTGSPEVGTLVQKAAA
                     VNHIGCVLELGGKSPQILFEDADIEAAAPVVVSAIVQNGGQTCSAGSRVLIQKSVYDT
                     FVAKIAARFTQLRVGSHAMDLDCGPLISANQKKRVQGFIEQAKSDGVPVLAQAQIADD
                     VPAGGFYVAPILFGPVPRANPLACEEVFGPVLSIIPF"
     gene            complement(1236..1898)
                     /locus_tag="VE030_05085"
     CDS             complement(1236..1898)
                     /locus_tag="VE030_05085"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZF22826.1"
                     /translation="MRKFLIAVAASALALGACKTIDPFTGEEKYGSTTKGAVIGAGAG
                     AVIGAISGRDAQDRRKRALIGAGVGALAGGAVGAYMDKQEADLRKQMQGTGVSVTRQG
                     ENLILNMPSNITFQTGSADMNASFFKVLDGVALVLKRYDKSVIEVAGHTDNVGKTDYN
                     QALSERRANAVAQYLISKGIKQERIITIGGGENHPVASNDTEAGRAQNRRVELTLMPL
                     KQ"
     gene            complement(2034..2648)
                     /locus_tag="VE030_05090"
     CDS             complement(2034..2648)
                     /locus_tag="VE030_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HZF22827.1"
                     /translation="MKADVDTVLFDLGAVLIDWNPRYLYQPLFNGDDQAMERFLAEIV
                     PPEWNLQIDAGKPFSEAVAERIRDHPQHAELIALWKDDWTKMLREPIAESVAILGELR
                     QRGHRLYALTNWSAETFPVARARFGFLEWFEDIVVSGEVKLAKPDPRIFTLAIERCRL
                     DPARTVFIDDSPHNVAAGSNAGLNALHFTGPKQLRSDLVRLGLI"
     gene            complement(2645..3361)
                     /locus_tag="VE030_05095"
     CDS             complement(2645..3361)
                     /locus_tag="VE030_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease activity regulator RraA"
                     /protein_id="HZF22828.1"
                     /translation="MKPEPIARETLEKLKRISTATLTSQLFKLGFRNTFLNGVKPLNP
                     HLRMVGEAATVRFVPAREDLASFDAIATNEYPQRKAIDEIRPGQVLVFDCRGVDSVAA
                     AGEILMSRLKVRGAAGAVTDGAMRDYSSIQALDFPVYAKGMAAPAHVHRHLAVDMNVP
                     VGCAEVLVMPGDVLVGDEEGVVCIPRHVADQVAQSGLELEQLEAFVLEKMKAGAPLRG
                     TYPPDAATRSEYEAWKNKQR"
     gene            complement(3467..4030)
                     /locus_tag="VE030_05100"
     CDS             complement(3467..4030)
                     /locus_tag="VE030_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22829.1"
                     /translation="MPGRSLIIAPGIVAAALSAAGAFDNSLLLAMSIEVPALVLLGWS
                     LAEIFPSPRCLERCNSGGLTGLLLAALLAALWMIPSAMDWVKIDGAMRVARIVSLIFG
                     VGYALRVSWPLAGTLIRWAWKLEAAGMMIRFGIAYLFVERTLCSGVSSSDQRAAGIVL
                     CVSGIAFLVGFNRKFMSPRESVNADGS"
     gene            complement(4034..4645)
                     /locus_tag="VE030_05105"
     CDS             complement(4034..4645)
                     /locus_tag="VE030_05105"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HZF22830.1"
                     /translation="MASTCVALAMGGSIWKATAGFRALALDDVRQIEFAGRQAPLPRA
                     TLTADDGRHFDIRQLAGRVAIVEFFYTRCPTVCSTLGSVLATVQREYPDAIAAGRLQL
                     LSISFDLAHDDSVAVAAYRRRFGSPAGWLTAIPDGPGSVRALQRTFGFLIQDTGDITV
                     AHSSKLYLISPHGVIVGAFDADESRELSAAVHGLLRPVGMQGV"
     gene            complement(4677..5264)
                     /locus_tag="VE030_05110"
     CDS             complement(4677..5264)
                     /locus_tag="VE030_05110"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZF22831.1"
                     /translation="MVPEHPSRIRERPEPYEEAHVIPAWLAILALVLAVWGVVYLALY
                     AGNDWGNYGDRRTLAALEARQTSAAGAGAMDGKALYMTHCSACHQASGLGVPGAFPPV
                     AGSGQVIGIPDNLVKIVLLGVTGQLTVKGVTYNGQMPSFAQLGDAEIAAIGSYERSSF
                     GNAAPPFDAALVARVRGELKGRTKPLAGDEELRLP"
     gene            complement(5288..5890)
                     /locus_tag="VE030_05115"
     CDS             complement(5288..5890)
                     /locus_tag="VE030_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013207902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HZF22832.1"
                     /translation="MNRTTDIIVGAILMLALVTMVLVVFPFAQLRPIKPPPGLKPYTE
                     AQLRGRAVYVSEGCMYCHSQQPRDPAQAPDMQRGWGRPSTPADYYYDDPVLLGTMRTG
                     PDLFNIGQRQPSKTWQLIHLYQPRAVVPGSIMPAFQYLFRVTGEKPTDVEPLALPEQY
                     QVPGYVVPTREALDLVEYLVSLDHTYPILQGDAAARDTAR"
     gene            complement(5887..7527)
                     /locus_tag="VE030_05120"
     CDS             complement(5887..7527)
                     /locus_tag="VE030_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HZF22833.1"
                     /translation="MELSGLGVPILVAVSFALSVTGLYIFIVALSHNLLWSQDEGANV
                     IFPPGEENAPEEPAASGEEARGLRAVTQGRQAQEGELADRIAADRSSATAVFVFLVLG
                     ALWLVIGSIAGLIASLTIHMPDWLAAYAPLTFGRMRVVHLNVVVYGWVSMTEMGIALW
                     LLPRMLRTELAASHYAIAGAAFWNSGLLLGVIAILFGWSDGLEYLEIPWQIASLFVIG
                     GALTAIPLFLTLARRRVQHLYVSVWYLGAALVWFPILFLIAKTPVHFGAEQAAMNWWY
                     GHNVLAYWMTPLALAAAYYLVPKVLGRPIYSYNLSLIAFWAQALFYGQAGVHHLIGGP
                     VPTWLVSVGVVASVMMVLPVLAFSVNYFQTLRGHWGTLRFSPTMRFVAIGAVSYVLVS
                     LQGSLEATRWFNTIVHFTHYTVAHAHLGLYGFVSFILFGAIYFAMPRIVDYEWPYPKL
                     IAAHFWLSAVGFGIYFVALTIGGYLQGLAMMDAKRPFMESVALTVPYLEARSLGGALM
                     VLGHLIFVFHFLVMALRLGPRRDAPPMLGLQIWGGRTL"
     gene            7732..>7971
                     /locus_tag="VE030_05125"
     CDS             7732..>7971
                     /locus_tag="VE030_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22834.1"
                     /translation="MPDIRIACAGPNRGSAVAVFETTGSAYRGNIKMNMGGKNMSMSE
                     TQVAEESATAASDPAIPQGIGPHSNVIMPIVRINVA"
BASE COUNT         1527 a         2638 c         2465 g         1341 t
ORIGIN      
        1 cgaacgggat gatcgacagc accgggccga acacctcttc gcaagccagt gggtttgcgc
       61 gcggcaccgg gccgaacagg atcggcgcca catagaagcc tcctgccggt acgtcgtcgg
      121 caatctgcgc ctgcgccagc accggcacgc catcgctctt cgcctgctcg atgaagccct
      181 gcacccgctt tttctgattg gcggagatca gcgggccgca gtccaggtcc atggcatgac
      241 tgccgacccg caattgcgtg aagcgcgccg cgatcttggc gacgaaagtg tcgtagacgg
      301 acttctggat cagcacgcgg ctgcccgcgg agcaagtctg cccgccgttc tgcacgatgg
      361 cgctcaccac caccggcgcc gccgcttcga tatcggcatc ctcgaacagg atctgcggcg
      421 acttgccgcc cagctcgagc acgcaaccga tgtgattcac cgccgccgcc ttctgcacca
      481 gcgtgccgac ttcgggcgaa ccggtgaaag acaggaaatc gattcccgca tggctcgcaa
      541 gcgccgcacc ggcctcttcg ccgatgccgg tcaccacgtt gatcgccccc tcagggaaac
      601 ccacgtcctg ggccagttgc gccaggcgca gcggcaccac gcaggcttcc tccgccggct
      661 tcatcaccac ggcgttgccc attgccagag cgggcgccag gctgcggccg aacatctgcg
      721 ccgggtagtt ccaggggatg atatggccgg ttacgccctt cggctcgcgc aggacggtga
      781 ccatgtagcc gtcgagaaac gggatggttt caccgtggaa tttgtcggcg gccgcgccgt
      841 agaactcgaa atagcgcgcc gccgcggtga tgtcggcccg cgcctgcttc atcggcttgc
      901 cgcagtctcg cgcttcgagc tgcgccaact cctcgtggtg cgccaggatc gcttcgccca
      961 gcttcgccat cagacgtccg cgctcgaccg cggtcatgcg accccacgcg ccttcctcga
     1021 aggcgcggcg tgcggccacg acggcggcgt ccacatcgga ccgggcgcca cgcgcgatgc
     1081 ttgcgaacgc ctgcccgtca ctggggctga tcatgtccag cgtgcggccg tcggaggctg
     1141 gctgccatct gcccccgatc agattctgaa atgccgtgac ggacggttcc gccacgcgtc
     1201 ttgcggtggt aacgctcatg aattcgattc tccggtcatt gcttcagagg catcagcgtc
     1261 aattccacgc gccggttctg cgcgcgcccg gcttccgtat cgttgctcgc caccggatga
     1321 ttctcgccgc ctccgatggt gatgatgcgc tcctgtttga tgcctttgct gatcaaatac
     1381 tgggcaaccg cgttggcgcg gcgctccgaa agcgcttggt tgtaatcggt cttgccgaca
     1441 ttgtcagtgt ggccggcaac ttcgattacc gacttgtcgt acctcttcag aaccagcgcg
     1501 acgccgtcca gaactttgaa gaacgaggcg ttcatgtccg cactgccggt ctggaaagtg
     1561 atgttgctcg gcatgttgag aatgaggttt tccccctggc gggtcaccga cacgccggtt
     1621 ccctgcatct gcttgcgcag gtcggcctcc tgcttgtcca tgtaggcgcc caccgcgccg
     1681 ccggcaagcg ctcccacgcc ggcgccgatc aacgcgcgct tgcggcgatc ctgcgcatcc
     1741 ctgccgctga tcgcgccgat gaccgcgccg gcgccggcgc cgatcaccgc acccttggta
     1801 gtcgagccgt atttttcttc cccggtgaac ggatcgatgg tcttgcacgc gcccaatgcg
     1861 agcgcgctgg cggcaactgc gatcaaaaac ttgcgcattc tggactcctt tgctggttct
     1921 tgtttcggtg gcggcgccgg caatgccgga atccgttcgt ccggcatttt accaataccc
     1981 ctgcacccga gcggacggat cacgcgccga cagcccggca ggcggcctgc gagtcagatc
     2041 aatcccaatc gcaccaaatc ggaccgcaat tgcttcgggc cggtgaaatg cagcgcgttc
     2101 aggccggcgt tgctccctgc cgcgacgttg tgcgggctgt cgtcgatgaa tacggtgcgg
     2161 gccggatcga gccggcagcg ctcgatggcc agcgtgaaga tgcgcggatc cggcttcgcc
     2221 agcttcactt cgccggacac gacgatgtcc tcgaaccatt cgagaaaccc gaaccttgcg
     2281 cgtgccaccg gaaacgtttc cgcggaccag ttggtcagcg cgtacagccg atgtcctcgc
     2341 tgccgcagct cgccgagaat ggcgacgctt tcggcgatcg gctcgcgcaa catctttgtc
     2401 cagtcgtctt tccacaaggc gatcaattcc gcgtgctgcg ggtgatcgcg gatgcgctcg
     2461 gccaccgcct cggagaacgg cttgccggca tcgatctgaa ggttccattc cggcggtacg
     2521 atctcggcaa ggaagcgctc catcgcctga tcgtcgccgt tgaacagcgg ctggtagaga
     2581 taacgcgggt tccagtcgat cagcaccgcg cccaggtcga acagcacggt atccacgtca
     2641 gccttcatcg ctgcttgttc ttccacgctt catactcgga tcgggtcgca gcatcgggag
     2701 gataggtgcc gcgcagcggc gcgccggctt tcattttctc cagcacgaag gcctcgagtt
     2761 gctccagctc cagtccgctt tgcgcgacct ggtccgccac gtggcgcggg atgcatacca
     2821 cgccctcctc gtctcccacc aggacatcgc cgggcatgac cagcacctcg gcgcagccta
     2881 cggggacgtt catgtccacc gccagatggc ggtgcacgtg tgccggcgcg gccatgccct
     2941 tcgcgtaaac gggaaaatcc agcgcctgga tcgagctata gtcgcgcatt gcgccgtcgg
     3001 tcaccgcgcc tgccgcgccg cgcactttca gccgcgacat cagaatttcg ccggcagcgg
     3061 cgaccgagtc cacgccccgg cagtcgaaca ccagcacctg ccccggccgg atctcgtcga
     3121 tggccttgcg ttgcggatac tcgttggtag cgatcgcatc gaagctggcg agatcctcgc
     3181 gtgccggcac gaaacgcacg gtcgccgctt ccccgaccat gcgcaggtgc ggattcagcg
     3241 gcttcacgcc attcaggaag gtgttgcgaa aaccgagctt gaacaactgc gaagtcagcg
     3301 tcgccgtgga aatccgcttc agcttctcca gcgtctcgcg ggcgatgggt tccggtttca
     3361 tcggctggtc ctccttgcgg gcgtaagcgc gactatccta acccagaaga aacaatactc
     3421 gctcggtacc catcaagcct ggccgcggct tgttgcaccg gagaactcac gatccatccg
     3481 cgtttacgga ctctcgcgga ctcatgaatt tccggttgaa cccgacaaga aaggcaatac
     3541 ccgaaacgca gagtacgatg cccgccgctc tctgatccga agacgaaacg cccgagcaga
     3601 gtgtgcgttc gacgaacagg taagcgatgc cgaaccggat catcattccc gccgcctcga
     3661 gtttccacgc ccagcggatg agggtgcccg caagtggcca gctcacgcgc agtgcatagc
     3721 cgacgccgaa aatgagcgag acgatacgcg caacacgcat cgcgccgtcg atcttcaccc
     3781 agtccatcgc ggacgggatc atccacagcg cggccaacag cgccgcgagc aagagcccgg
     3841 tgagaccgcc ggagttgcag cgctccaggc agcggggcga ggggaatatc tcagcaaggc
     3901 tccatcccaa aagcaccaga gcgggaacct cgatgctcat cgcaagcagc aggctgttgt
     3961 cgaaggcgcc cgcagcgctc aacgctgcag cgacgatgcc tggagcaatg atcagtgatc
     4021 gaccgggcaa cacctatact ccctgcatgc cgacaggccg gagcaagcca tgcacggcgg
     4081 cgctcagctc cctgctttcg tcggcatcga aggcgccgac aatcactccg tggggcgaga
     4141 tcagatagag cttggatgaa tgcgccaccg tgatatcgcc cgtgtcctga atcaggaatc
     4201 cgaaggttcg ctgcaacgcg cgcacgctgc caggcccgtc agggatcgcc gtgagccagc
     4261 ccgcagggct gccgaagcgg cggcgatacg ccgcgaccgc aaccgagtcg tcatgtgcga
     4321 gatcgaagga aatgctcagc aattgaagtc gcccggcggc gatcgcatcg gggtactcgc
     4381 gttgcaccgt tgcgagcacg ctccccagcg tcgagcagac ggtggggcag cgggtataga
     4441 agaactcgac gatagccacc cggccggcaa gctgccgaat gtcgaagtgt cggccgtcat
     4501 cggcggtcag cgtcgcccgc ggcaacggcg cttggcgccc ggcaaattcg atttgcctga
     4561 cgtcgtcgag cgcgagcgcg cggaagccgg cggtcgcctt ccagatactg ccgcccatcg
     4621 cgagcgccac gcaggtgctt gccacgagcg tccatccgag cgagctcgtg ctccgcttac
     4681 gggagtcgaa gttcctcatc gccggccagg ggcttggtgc gtcccttcaa ttcgccgcgt
     4741 acccttgcca ccagcgccgc gtcgaacggc ggcgccgcgt tgccgaagct gctgcgttcg
     4801 tagcttccga tcgcggcgat ttcagcatcg ccaagctggg cgaacgaagg catctgaccg
     4861 ttgtatgtga cgcctttgac ggtgagctgg ccagtgacgc caaggagcac gattttgacc
     4921 agattgtcgg ggatgccgat gacttgcccg gatccggcga caggcggaaa tgcgcccgga
     4981 acgcccagcc cggatgcctg gtggcaagcg ctgcaatgag tcatgtacaa cgccttgccg
     5041 tccatcgcgc cggctccggc cgcgctcgtc tgccgagcct cgagcgccgc cagcgtgcgg
     5101 cgatcgccat aattgcccca gtcgttgccg gcgtacaacg ccaggtaaac cacaccccac
     5161 accgccagca ccagcgcgag gatcgcaagc cacgccggga ttacgtgcgc ttcctcgtaa
     5221 ggctcgggac gctctcggat ccgggaggga tgctcgggaa ccatggctcg cgctctcctc
     5281 tatggaccta tcgcgccgtg tcccgagcgg cagcatcgcc ctggagaatc gggtaggtat
     5341 ggtcgagaga gaccaggtac tcgaccagat cgagggcctc gcgggtcggc accacatagc
     5401 ccggcacctg gtattgctcc ggcagcgcga gcggctcgac atctgtcggc ttctcgcccg
     5461 tcacccggaa caggtactga aaggcgggca tgatgctgcc cggcacgacg gcgcgcggtt
     5521 gatagagatg aatcagctgc caggttttgc tgggttgccg ctggccgatg ttgaacagat
     5581 cgggaccggt gcgcatggtg ccgaggagga ccggatcgtc atagtagtag tccgcaggcg
     5641 tggacggacg accccagccg cgctgcatgt ccggcgcctg cgccgggtcg cgcggttgtt
     5701 gactgtggca atacatgcag ccctcgctca cgtagacggc gcggccgcgc agctgcgcct
     5761 cggtgtacgg cttgagaccc ggcggcggtt tgatcgggcg caactgcgcg aatgggaaca
     5821 cgaccagaac catagtcacc agcgcgagca tcaggattgc gccaacgatg atgtccgtgg
     5881 tgcggttcat aacgttctcc caccccagat ctgcaagccg agcatcggcg gcgcgtcgcg
     5941 acgcggcccc aagcgcagcg ccatcacgag gaaatgaaat acgaagatca ggtggccgag
     6001 caccatcaag gcaccgccga gcgagcgcgc ctcgaggtag ggaacagtca gcgccaccga
     6061 ctccatgaac gggcgcttcg cgtccatcat ggcgagcccc tgcaggtatc cgccgatggt
     6121 caacgcgacg aagtagattc cgaatcccac cgccgacagc cagaagtggg cagcgatcaa
     6181 cttcggataa ggccactcgt aatccacgat gcgcggcatg gcgaagtaga tcgcgccgaa
     6241 caggatgaaa ctcacgaagc cgtagagccc gagatgggca tgcgcaaccg tgtagtgggt
     6301 gaagtgcacg atggtgttga accagcgcgt cgcctccagc gacccctgga gcgagactag
     6361 cacgtagctg accgcgccga tcgcgacgaa gcgcatggtt ggggaaaagc gcaacgttcc
     6421 ccaatggccg cgcaaggtct ggaaatagtt cactgagaaa gccagcaccg gcagaaccat
     6481 catcacgctc gcgaccacgc cgacactcac cagccaggtc ggcacggggc cgccgatgag
     6541 atggtgcacg cccgcctggc cgtagaacag cgcctgcgcc cagaacgcga tcagtgaaag
     6601 gttgtacgag tagatcggcc tgccgagcac tttcggcacc aggtagtagg cggcagcgag
     6661 cgcgagcggc gtcatccagt atgcaagcac gttgtgtccg taccaccagt tcatcgccgc
     6721 ctgctctgcg ccgaagtgca ccggcgtctt cgcgatgagg aacagaatcg ggaaccacac
     6781 caatgccgcg ccgaggtacc acaccgacac gtacaagtgc tggacccgac gccgcgccag
     6841 cgtcaggaaa agcggaatcg ccgtaagcgc gccgccgatg acgaagagcg acgcgatctg
     6901 ccacgggatc tcgagatatt cgagtccgtc gctccagccg aaaagaatgg cgatcacccc
     6961 gagcagcaga cccgaattcc agaatgcggc gccggcaatg gcgtagtgcg aggccgccag
     7021 ctcggtgcga agcatccgcg gcaggagcca cagcgcgatt cccatctcgg tcatcgagac
     7081 ccagccgtag accacgacgt tcagatgaac cacgcgcatg cggccgaagg tgagcggagc
     7141 gtaagccgcc agccagtcgg gcatgtggat cgtgagcgat gcgataagcc cggcgatcga
     7201 accgatcacc agccataggg cgccgagcac caagaagacg aaaacggcgg tcgcggatga
     7261 gcggtcggcc gcaatacgat ccgcgagctc gccctcttgc gcttgccgcc cctgcgtgac
     7321 tgcccgcagc ccgcgggcct cttcgccgct cgctgccggt tcctcgggag cgttctcttc
     7381 accgggagga aagatgacgt ttgcgccttc gtcctgcgac cacaaaaggt tgtgcgagag
     7441 cgcaacgatg aagatgtaca ggccggttac agaaagcgca aagctaaccg caaccaggat
     7501 gggaacaccg agaccggaca gttccatgcc acaccccgca tcctaattcc gcaccttggc
     7561 cggatcgatc gatccggcca ccgctcggat tcacctccca agactctggt cgagcctgga
     7621 gccatctttg cgtggggact gcgggtgttg ctcgtggcct tgccagggac taccaggtag
     7681 caagggccac tatatagcag gacaatcggc ggcaggaaca aaagtgtcaa catgcctgac
     7741 atccgcatcg cgtgcgccgg accgaaccgg ggcagcgcag tagccgtgtt cgagaccacg
     7801 ggcagcgcgt atcgcggcaa catcaaaatg aacatgggcg ggaagaacat gtccatgtcc
     7861 gagacgcagg tggcagaaga atctgcgacc gccgctagcg accctgcgat accccagggc
     7921 atcggtccgc acagcaatgt cataatgccc attgtccgga tcaatgtcgc c
//
LOCUS       DASQOP010000127         4315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1060194, whole genome shotgun sequence.
ACCESSION   DASQOP010000127 DASQOP010000000
VERSION     DASQOP010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060194"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..875)
                     /locus_tag="VE030_05130"
                     /pseudo
     CDS             complement(1..875)
                     /locus_tag="VE030_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800401.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
     gene            complement(950..2548)
                     /locus_tag="VE030_05135"
     CDS             complement(950..2548)
                     /locus_tag="VE030_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZF22835.1"
                     /translation="MQGRLKFWGWGLESEVLRPEEVSALEAAYAHRFGIAAYDVTPAP
                     RAEEIRLRAPRVTPPESLREVCSTDHYQRLLHSYGRSFFDSARIFARDFANPPDVVAF
                     PRDEREIVAVLDWCDSVNAVAIPWGGGSSVVGGVEPPRTDRYVVTIDLKHLGRVLEID
                     AVSQAARIQGGTFGPALESQLKPSGLTLRHFPQSFEFSTLGGWIATRSGGHYATLYTH
                     IDDFVESLRVVTPAGTLESFRLPGSGAGPSPDRLFIGSEGILGIITEAWMRLRPRPTF
                     RAATSARFKDFYQAAQCVRAIGQSHLYPANTRLLDAGEALVNGAGDGRHSILVLTFES
                     ADRPQEENLQRALEIVRANGGEYDKRALNTDGAQRQGAAGQWRDSFLRMPYYREVLTP
                     RGIIADTFETSITWDRFPDFHARVKAATERAIEEVTGKKGVVTCRFTHVYPDGPAPYF
                     SFHALGDKRRLVEQCWAIKIAASEVLNALGGTITHHHAVGRDHRRWYDKQRPELFAGA
                     LRAAKKSLDPRGMMNPGVLVDPLA"
     gene            complement(2558..3010)
                     /locus_tag="VE030_05140"
     CDS             complement(2558..3010)
                     /locus_tag="VE030_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HZF22836.1"
                     /translation="MNAFRSVLLGAAVLALLVGNAFADIKSPVGVWRSIDDTTKKERS
                     IIRITEVNGEFSGVVEKLFDQPGDDPAHLCKDCKGERKDRPIIGMTILWGLKKDGDTW
                     AGGEILDPHNGKTYRCKMTLSNDGKSLNVRGFIGISLFGRSQIWLREQ"
     gene            complement(3190..>4315)
                     /locus_tag="VE030_05145"
     CDS             complement(3190..>4315)
                     /locus_tag="VE030_05145"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HZF22837.1"
                     /translation="RNVRPDDMLAHVLTSVLAQAPGLDPSAIDDVVVGCAMPEAEQGM
                     NVARIGLLLAGFPNSVSGMTINRFCSSGLQAVAIAADRIRLGEAEVMIGAGTESMSMI
                     PMGGNKIAANEQIFLKDENVGIAYGMGITAEKVAEQWKVSREAQDQFATESHRRALAA
                     IEKGEFGDEITPYTVNERIPDFEHHQVATRSKVVSVDEGPRAGTTAEVLAKLRPAFAA
                     NGTVTAGNSSQTSDGAGAVILASEAAVKRLNLQPLGRFLGFAVAGVPPEIMGIGPKFA
                     IPKVLKQVGLKQDALDWIELNEAFAAQSLAVIGDLGLDRTKVNPLGGAIALGHPLGAT
                     GAIRTATLLHGMRRRGQKYGMVTMCIGTGMGAAGVFESLR"
BASE COUNT          731 a         1420 c         1349 g          815 t
ORIGIN      
        1 gccgggcaga acgaagcccg ggttgtagaa ctcgaaaagc agcccgtaca cgccgagcag
       61 cagcaggatg taggctatgc tcggatcggt gatcagcgca ggaagcgact gcgccagtcc
      121 ggctcaatcg caatggtctc catgcccttg gtccgcagga tccgatccag ccccgcgacg
      181 ttgagcccgc ggccgtcgag cttcttgaga agatcgggca ggtcggcagc gaccaggtcg
      241 atcaccttga gcttcagcgc ttcctcggct gacaggctta ccgcctcgcg cacggccttc
      301 tccgcccact cggcattacg gccgcgcaat tgggccagtc cgcgaatgta agccgccgcg
      361 tcgaacaccg ccttgcgccg atgcgcatca ctcatccagt ccttctcggc atccttggtc
      421 tggtctgcct tttttccggt cttgcgctcc ggttcctgcg gctggcgctg cggttccggc
      481 gatccgggca tgccaccgat cgagatgggg gttgccgcgc ccaggtttgt ggcgggcgcc
      541 atcgcggcga catggctggc gtaaagaatg taggtgccag cgctggcggc gcgcgcaccg
      601 ctcggcgcca caaaagtggc cacaggaacg gatgaagcga ggatgtcctt gatgatcgag
      661 cgcatcgagg tgtcgagccc ccccggagta tccattttca gcaccaccag ttgcgcctgc
      721 tgttccgcgg cccgctcgag attgcggtgg acatagtcgg cggtcgcggg gccgatcgca
      781 ccgttgagcg acagcgccac gaccgggccc tccgcgccga gcgccaggcc cgcgcccaac
      841 aacaagagga tcagcgcaac taaaagaact cgcatcggat caattgcaac gtgggttttt
      901 gctgcgtcca ctatagtcct aacaccggcg caagcaaacg cgcgaagagt caggcaagcg
      961 gatcgaccag gactccggga ttcatcatgc cgcgcggatc gagggacttt ttcgcggcgc
     1021 gcagcgcgcc ggcaaacaac tccggccgtt gtttgtcgta ccagcgccgg tggtcgcgtc
     1081 cgaccgcgtg gtgatgggtg atggtgccgc cgagcgcgtt gagcacctcg gaagccgcga
     1141 tcttgatcgc ccagcattgc tcgaccagtc ggcgcttgtc gccaagcgcg tggaaactga
     1201 aatagggcgc cggcccatcc gggtagacgt gggtgaagcg gcaggtgacc acgccttttt
     1261 tcccggtgac ttcttcgatt gcacgctcgg ttgcggcttt gaccctggca tggaaatcgg
     1321 gaaagcggtc ccaggtgatc gatgtctcga aggtgtcggc aatgattccg cgcggcgtca
     1381 ggacttcgcg gtagtagggc atgcgcagga agctgtcgcg ccattgtccg gccgctccct
     1441 ggcgctgtgc tccgtcggta ttaagcgccc gtttgtcgta ctcgccgccg tttgcgcgca
     1501 cgatctcgag cgcgcgttgc agattctcct cctgcggccg gtccgcggat tcgaaagtca
     1561 gcaccagaat cgaatgcctg ccgtcgccgg cgccgttcac caacgcctcg ccggcatcga
     1621 gcagtcgcgt attggccgga tacagatgcg actggccgat ggcgcgcacg cactgcgccg
     1681 cctggtaaaa atccttgaat cgcgcggagg tcgcggcgcg aaaggtcggg cgcggccgca
     1741 gccgcatcca ggcttcggtg atgatgccga gtattccttc ggaaccgatg aaaagcctgt
     1801 cgggactggg cccggcgccc gagcccggca gccggaaaga ttccagggta ccggccggag
     1861 tcaccacgcg cagactctcg acgaaatcgt caatgtgggt gtagagcgtg gcgtagtggc
     1921 cgccgctgcg ggtggcgatc cagccgccga gcgtcgagaa ctcgaaggac tgcgggaaat
     1981 ggcgcagcgt caggccgctc ggcttgagct ggctttccag cgccggcccg aaagtgccgc
     2041 cctgtatgcg agccgcctgc gaaaccgcgt cgatctcgag cacgcggccg aggtgcttca
     2101 ggtcaatggt gaccacgtac cggtcggtgc gcggcggctc gacaccgccg accacgctcg
     2161 aacctccgcc ccagggaatc gcaacggcgt tcaccgaatc gcaccagtcg agaacggcga
     2221 cgatctcgcg ctcgtcgcgc gggaaagcca ccacatccgg cggattggcg aagtcgcgcg
     2281 caaaaatcct ggcgctgtcg aagaaagacc ggccgtagct gtgcagcagc cgctggtaat
     2341 ggtcggtgga gcacacttcg cgcaggcttt ccggcggcgt cacgcgcggt gcgcgcaagc
     2401 ggatttcctc tgcgcgcggg gccggagtca cgtcgtaagc cgcaataccg aaacgatgcg
     2461 cgtatgcagc ctcgagcgcg ctcacctcct ccgggcgcag cacctcgctt tccaggcccc
     2521 agccccagaa cttgagcctc ccctgcaccg gcgtttccta ttgctcgcgc aaccagatct
     2581 gcgagcgtcc aaacagcgat ataccgataa agccgcgcac gttcaggctt ttgccatcat
     2641 tggagagcgt cattttgcat cgataggtct tgccgttatg gggatccagg atttctccgc
     2701 cggcccaggt atcaccgtct tttttcagcc cccacaggat ggtcatgccg atgatcggtc
     2761 tgtccttgcg ctcgcccttg caatccttgc acaggtgcgc gggatcgtcg ccgggctgat
     2821 cgaagagctt ctccaccacg ccactgaatt cgccgttgac ttctgtgatg cggatgatcg
     2881 agcgctcctt cttggtcgtg tcgtcgatgc tgcgccatac tcccactgga gacttgatgt
     2941 cggcaaaagc attccccacc agcaacgcca gcactgcggc gccgagcaag acggacctga
     3001 acgcattcat gattgattcc cctccttgtg gttgcgcccg gcaaccgggc gcgccggggt
     3061 cgccccggca ggttccggcc gggcaattct atcgccgtgt cattccttgc tgcaccttcg
     3121 cctgatacgt ctcttttgct gcattctgca tagagcgccg accgacagtg cgctgggccg
     3181 tgccagcgtt caccgcagag actcgaagac gccggctgca cccatcccgg taccgatgca
     3241 catcgtgacc atgccgtact tctggccgcg ccggcgcatc ccgtgcagga gcgtcgcggt
     3301 gcggatcgcg ccggtggcgc ccagcggatg accaagtgcg atcgctccgc ccagcggatt
     3361 caccttggtc cggtcaagcc cgaggtcacc gatcaccgcc aggctctgcg cagcaaatgc
     3421 ctcgttcaac tcgatccagt ccagcgcatc ctgtttcagc cccacctgct tcaacacctt
     3481 gggtatggcg aacttcggac cgattcccat tatttccggc ggcacgccgg ccaccgcgaa
     3541 gccgagaaag cgtcccagcg gttgcaggtt gaggcgcttg acggcggctt cgctggcgag
     3601 aatcaccgcc ccggccccgt ccgaggtttg cgaactgttg ccggcggtga cggtgccgtt
     3661 ggcggcgaac gcgggcctca gtttcgccag cacttccgcg gttgtgccgg cacgcgggcc
     3721 ttcgtctacg ctgaccactt tgctgcgggt cgcgacttgg tggtgttcga aatctgggat
     3781 gcgttcgttg acggtataag gcgtgatctc gtctccgaac tcgccctttt cgatcgccgc
     3841 aagcgcgcgc cggtgcgatt cggtggcgaa ctggtcctgg gcctcgcggc tgactttcca
     3901 ttgctccgcc accttctccg cggtgatgcc catgccgtag gcgatgccga cgttttcgtc
     3961 cttgaggaag atctgctcgt tcgcagcgat cttgttgccg cccatcggaa tcatgctcat
     4021 gctttcggtg ccggcgccga tcatgacttc ggcctcgccg aggcggatgc ggtccgctgc
     4081 gatcgccacc gcctgcaatc ccgacgaaca aaatcgattg atggtcattc ccgaaacgct
     4141 gttggggaat ccggcgagga gcaggccgat acgcgcgacg ttcatcccct gctcggcctc
     4201 cggcattgcg caaccaacca cgacgtcgtc tatcgccgaa ggatccagcc cgggagcctg
     4261 cgcgagcacg ctcgtcagca catgcgccag catgtcgtcg gggcggacgt tcctg
//
LOCUS       DASQOP010000128        12674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2065823, whole genome shotgun sequence.
ACCESSION   DASQOP010000128 DASQOP010000000
VERSION     DASQOP010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12674
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2065823"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1237
                     /locus_tag="VE030_05150"
     CDS             <1..1237
                     /locus_tag="VE030_05150"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZF22838.1"
                     /translation="GQWQDDPWPDLWRVPAASLLIGLLLEGVAARRRGIRISPRIPER
                     VMLGQPATAELVLGNDGAGPLQVELQQIFSNGLRGSDAIAAYIVPAHGTAAHAFSFTA
                     QKLGTLEWQPVYTRTRGLFGLAWWSRKFDIPQRLKVVPARLRGAERGPGTRPQGERAQ
                     RGAGAGFELLGFRDYRPGDPMRAIDWKATARSQRYTVRMHSEERNLELLVLLDAGRTS
                     QLQAGTLSRLHHYVNVAARLTERAILNGDHVGIIVFADRPLAALPPAHGAAALLGVRR
                     ILEQARPVGTESNPLAAALLARRLLHQRSLVVLLTDVDESQDPAQLLRAVQLLAPKHL
                     PLIAGTLDEETLALRHRQSSHWLHPYQAFAAAEMLQSVRGMTLRLRRSGAQAVTTVPA
                     QLDGAVLRSYEVLRSQRQI"
     gene            complement(1220..2218)
                     /locus_tag="VE030_05155"
     CDS             complement(1220..2218)
                     /locus_tag="VE030_05155"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HZF22839.1"
                     /translation="MASRPEAALTAWLQGRAAQWQRLSRILAEVSSRRQAELPQLKEL
                     VSGYRALARDVSLARAAAPDAKLVARLEALLTQAREVIHRRPGNARDALAGLFRDGAA
                     RAAFELRGRIAAVTALFLASAAAGWWLVHSYPELAALFASEQMINRVQRGELWTDGLI
                     NIVPSALLSLGIMTNNIVVSLFAFTLGALYGLGTLYIVGMNGLMLGGVFALTAQHGLA
                     GWLAEFVVAHGMVEISVILLAAAAGVELGEALARPGNRSRAAAFRDAVRRAGQLLPVC
                     VVFLVGAGLIEGYVSPDPHFTMAQRVTIGVVYWLFLLLVLSGRLRRRPHGRSDLPL"
     gene            complement(2202..2945)
                     /locus_tag="VE030_05160"
     CDS             complement(2202..2945)
                     /locus_tag="VE030_05160"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HZF22840.1"
                     /translation="MEDKLVVQAADAVEVELPVAGIGSRSYAFLIDWHLRFLLALAWL
                     ALVAVLVGLLSPAEDFFSPLLKAGKVALYAALLPAAAIYFLYHPLLEIVMQGRTPGKR
                     MAGIRIVTERALTPDAGALIIRNVFRLVDSLPAFYVLGLVVAMFTARQVRIGDLAAGT
                     LLVYDTGKRSLDFDGVIGFSSSRLSPADRELLDDLLERWRGLDAPLRRGLAEKFLARI
                     GEPVAQGGTESELRKRLRKLRDESRGKPA"
     gene            complement(2966..4630)
                     /gene="recN"
                     /locus_tag="VE030_05165"
     CDS             complement(2966..4630)
                     /gene="recN"
                     /locus_tag="VE030_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008325483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HZF22841.1"
                     /translation="MLRSLSIRDFVIVDKLDLEFQPGFTALTGETGAGKSILIDALAT
                     VLGSRAESGQVREGKDKAEVSAEFSTGEAPAVSDYLTENDLSGDNGVCLLRRVIESSG
                     RSRAYVNGRPATVQQLREIGERLVDIHGQHAHQSLLRPPAQRDLLDGHAGAADLAYEV
                     AEAFRAWQDIRHRRLALETDAQALEAEREQLQWQVKDLDALGFRAEEWEELQAEHKRL
                     AHAASLIEAVQFSLDVLSEGEMSTLAAVASVNSRLDGMTDFDPQLKEILAALEPAQIQ
                     LQEAVYGLRHYQSRLDLDPQRLREVETRLDAVHTASRKFRVNPLEMPERLAKMKARLT
                     ELETGFDPEALRQRENLASAHWQERARKLSAARRKAAADFAKRVTASMQSLAMGGGAF
                     EIALHPLSEGNAHGLEQIEFLVAGHAGATPLALAKVASGGELSRISLAIQTVTSQVAA
                     VPTLIFDEVDAGIGGRVAEIVGRMLKELGSRHQVMCVTHLPQVAAAGNQQWQVSKSAA
                     DGKVASQVAVLDRASRVEEIARMLGGVKITETTRKHAAELLGFGAR"
     gene            complement(4667..5539)
                     /locus_tag="VE030_05170"
     CDS             complement(4667..5539)
                     /locus_tag="VE030_05170"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HZF22842.1"
                     /translation="MSSAFKTIALIGKYKSPEIAEPLLRLGDFLKGRGVRIVLDPLTA
                     AHIGKNNHAVLPLEDVGRTAELAIVIGGDGTMLNIARTFAPYNVALVGINQGRLGFLT
                     DLSMDTMFEAIGSILDGSFITETRMLLQAAVLSGERQVLDVLAFNDVSVNKGAEGSLI
                     EIEVHIDGQFVYNLRSDGLIVASPTGSTAYALSSGGPILHPSLNVIGLVPVCPHTLSN
                     RPIVVSSESVIEILIYKAADARAHFDSHSHFELQERDKVIVRRCPHAIRLLHPLGHNY
                     YHMLREKLHWSETL"
     gene            5636..6673
                     /gene="hrcA"
                     /locus_tag="VE030_05175"
     CDS             5636..6673
                     /gene="hrcA"
                     /locus_tag="VE030_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HZF22843.1"
                     /translation="MATNDSTPSERAQILLKLLVERYITDGQPVGSRALAKFSGLDLS
                     PASIRNIMADLEELGFIASPHTSAGRIPTPRGYRFFVDTLLKVRPLDQMEIDQLEGQL
                     QPDHTQKLVSQASHLLSDLTRFAGVVMTPKRRSAAFRHIEFLRLSEKRILLIIVAPDG
                     DVQNRIILTDRAYEPSELTEAANFLNQNFAGLTFDEIKARIHDELRKLREDMTRLMTQ
                     ALEASTEVMNQNSEDYVLSGEHNLLHVQDLSSNMASLRRLFDMFEQKTSLLQLLDISS
                     RAHGVQIFIGGESGLVPLDECSVVTAPYEVDGQVVGTVGVVGPTRMAYERVIPIVDIT
                     AKLLSSALSQH"
     gene            6769..7872
                     /gene="hemH"
                     /locus_tag="VE030_05180"
     CDS             6769..7872
                     /gene="hemH"
                     /locus_tag="VE030_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HZF22844.1"
                     /translation="MSFLPEPRFQHGTIAKAGILLINLGTPTAPTARALKPYLKQFLS
                     DPRVVEIPRLVWWPILNGIILNTRPKQSAAKYAKIWTRDGSPLRVHTEKQATLLKGYL
                     GERVKTPHSIAWAMRYGEPSIGTVLRELRGQGCDRIVVLPLYPQYAASTTASAFDAVF
                     DAVKRMRNAPALRLVKHYHDHPGYITALAKDINDYWMANGRPDKLVMSFHGVPRFTLD
                     RGDPYHCECQVTGRLLAQELGLKPEQYMVTFQSRFGRSEWLKPYTIDTIGTLGKRKTS
                     RVDVVCPGFVSDCLETLEEIGIENKAAFLKAGGGEFHYIPCLNQRHEWIQALARIAAE
                     NLHGWASTDWDQAPALRAAAESRTRAVAMGAKN"
     gene            8096..8665
                     /gene="grpE"
                     /locus_tag="VE030_05185"
     CDS             8096..8665
                     /gene="grpE"
                     /locus_tag="VE030_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HZF22845.1"
                     /translation="MQDQEKLAAVPQNDSGTASLAAEDAGYDAPAVETMPGMEELLKK
                     AELAAQEHHDAWLRAKAETENVRRRTQTDIANAHKYALEGFASELLAVRDSLEAALAP
                     GNATPDGLRSGVELTLKQLTNAFEKFSIKEINPLGEKFDPHKHQGIGLVEADADPNTV
                     VSVLQKGYSLNDRVIRPALVTIAKSRASA"
     gene            8732..10666
                     /gene="dnaK"
                     /locus_tag="VE030_05190"
     CDS             8732..10666
                     /gene="dnaK"
                     /locus_tag="VE030_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HZF22846.1"
                     /translation="MGKIIGIDLGTTNSCVAVMENGQPKVIENSEGARTTPSIVAYME
                     DGEILVGAPAKRQAITNPKNTLFAVKRLIGRRFIEPEVQKDIDLMPYKIVQNSNGDAW
                     IEVRGKKISPPEVSAQVLVKMKKTAEDYLGEPVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKKEGDRKIAVYDLGGGTFDISIIEIAVVDKEHQFEV
                     LSTNGDTFLGGEDFDQRLMDYLADEFKKEQGIDLRKDVLALQRLKEAAEKAKIELSSS
                     QQTEINLPYITADASGPKHLAIKITRAKFESLVDELIQKTVEPCRTAIKDAGVKLSDI
                     SDVILVGGMTRMPKVQEKVKEIFGKEPRKDVNPDEAVAIGAAIQAGVLQGDVKDVLLL
                     DVTPLSLGIETLGGVMTKLIQKNTTIPTKAQQVFSTADDNQTAVTIHVLQGEREMASG
                     NKSLGQFNLSDIPPAPRGLPQIEVTFDIDANGILHVSAKDKATGKENKIKIQANSGLK
                     EDEIQRMVKDAEAHADEDRKARELVEARNQCDALIHAVKKSLTEHGDKVTADEKSGIE
                     AAIKAAEEAIKSGDKDVIEAKSKALAEASHKLAEKMYSQQQAQGGGAQPQSESGGAKP
                     EDGNVVDAEYEEVKDQKKSA"
     gene            10765..11889
                     /gene="dnaJ"
                     /locus_tag="VE030_05195"
     CDS             10765..11889
                     /gene="dnaJ"
                     /locus_tag="VE030_05195"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HZF22847.1"
                     /translation="MSKRDYYEVLGVNRDASEEEIKKAYRKLAMKHHPDRNPDNPKSE
                     GLFKEAKEAYEVLSDAGKRGAYDQYGHAGVDPRAGMGAGAAGAGFGGFADAFGDIFGD
                     IFGAGRQRSNVYRGADLRYNLEISLEEAARGTETRVRIPTMEECVTCGGSGAKPGTQP
                     MTCPTCGGQGQVRITQGFFSIQQTCPKCHGSGKVVQSPCATCNGAGRVKRQKTLSVKI
                     PAGVDEGDRIRLSGEGEAGVNGGPSGDLYVVIHIRAHEVFSREGNDLHCEMPISLTKA
                     ALGGEIEIPTLDGYAKIKIPLETQTGKVFRLRGKGIKGVRSNAYGDLLCHVVVETPVN
                     LTPRQKELLQELEEINDRDNGRHNPRAKSWMDRVREFFAE"
     gene            complement(11951..>12674)
                     /locus_tag="VE030_05200"
     CDS             complement(11951..>12674)
                     /locus_tag="VE030_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DALR domain-containing protein"
                     /protein_id="HZF22848.1"
                     /translation="YLGSHFDIHGGGQDLQFPHHENEIAQSEGAHGHAFVNYWMHNGF
                     VRVDNEKMSKSLGNFFTVREILTKYDAEVVRFFIVRAHYRSPLNYSDQHLDDARQALT
                     RLYTALKAVPAQAGDVDWNEPQAARFQAAMNDDFNTPEAVAVLFDLANEVNRSRSSNT
                     AALLKSLGAVLGLLQRDPDEFLRGDLPAGWTVARVEEMIAARAQAKKARNYAESDRIR
                     DQLLAAGIVLEDGPAGTTWRRK"
BASE COUNT         2524 a         4095 c         3993 g         2062 t
ORIGIN      
        1 cggccagtgg caggacgatc cctggccgga cttgtggcgc gtgccggcgg cgagcctgct
       61 gatcggactg ttgctggaag gcgtggccgc acggcggcgc ggaatcagaa tctcgccccg
      121 catcccggag cgcgtcatgc ttggccagcc ggccactgcg gaactggttc tcggcaacga
      181 tggcgcaggg ccactccagg tcgagctgca gcaaattttc tcgaacggcc tgaggggcag
      241 cgacgcgatc gcggcctaca tagtgcctgc gcacggcacg gcggcgcacg cgttctcttt
      301 tacggcgcag aaactgggca cgctggagtg gcagccggtc tatacccgca cgcgcggcct
      361 gttcggtctt gcctggtggt cgcgcaagtt cgacattccg caacgcttga aggtagtgcc
      421 ggcgcggctg cgcggtgccg agcgcggacc cggcacccgg ccccaggggg aacgcgcgca
      481 acgcggcgcg ggcgccggct tcgagctgct cgggttccgg gactatcggc ccggcgatcc
      541 gatgcgggcg atcgactgga aagccaccgc gcgcagccag cgctatacgg tgcgcatgca
      601 cagcgaagag cgcaacctgg aattgctggt gttgctggac gcgggcagga cctcgcaact
      661 gcaggccggg acgctgagcc ggctgcacca ttacgtcaac gttgcggcca ggctcacgga
      721 aagagcaatc ctgaatggcg atcacgtcgg aatcatcgtg ttcgccgacc gtccgttggc
      781 ggcactgccg ccggcacacg gggccgcggc cctgctcggg gtgcgccgca ttctcgagca
      841 ggcgcgtccg gttggcaccg aatccaaccc gcttgcggcg gcactgctgg cgcgacgcct
      901 gctgcaccag cgcagcctgg tggtgctcct caccgacgtg gacgaaagcc aggatcctgc
      961 gcagctgttg cgcgccgtac agttgctggc gcccaagcat ctacccttga ttgcgggcac
     1021 tctcgatgag gagacgctgg cgttgcgcca ccggcaatcc agccactggc tccatcccta
     1081 ccaggccttt gccgccgcag aaatgctgca gtcggtgcgg ggcatgactt tgcggttgcg
     1141 ccgcagcggt gcgcaagcgg tcacgaccgt accggctcag ctcgatggcg ccgtgttgcg
     1201 ctcctacgaa gtcctgcggt cacagcggca gatctgaacg gccgtgcggc cgccgccgca
     1261 acctgccgct gagcaccagc aagaggaaca gccaatagac cacgccgatg gttacgcgct
     1321 gtgccatggt gaaatgcgga tcgggcgaaa cgtatccttc gatcaatcct gcgccgacca
     1381 ggaaaaccac gcacacaggc agcaattgtc cggcgcggcg caccgcgtct cggaacgccg
     1441 cggcgcggct gcggtttccc ggacgcgcca gcgcctctcc gagttcgact ccggccgctg
     1501 cggcgagcag gatcaccgag atttcgacca taccatgcgc gaccacgaac tcggcaagcc
     1561 agcctgccag gccatgctgc gccgtcagcg caaacacccc gcccagcatc aacccgttca
     1621 tgccgacgat gtatagcgtg cccaatccgt acagagcgcc gagcgtaaac gcgaacagcg
     1681 aaaccacgat gttgttggtc atgatgccca gcgacagcag cgccgacgga acgatgttga
     1741 tcaatccgtc ggtccagagt tcgccgcgct gcacgcggtt gatcatctgc tcggaagcga
     1801 acagcgccgc cagctccggg taactgtgca ccagccacca gccggcggcg gcgctggcga
     1861 gaaagagcgc cgtgaccgcg gcgatgcggc cgcgcaactc gaacgccgcc cgcgcggcgc
     1921 cgtcgcgaaa caatccggcc agggcgtcgc gcgcattgcc cggcctgcgg tggatcacct
     1981 cgcgcgcctg cgtaagcagc gcctccagcc gcgccaccag cttggcgtcg ggcgccgctg
     2041 cgcgcgccag cgacacgtcg cgcgccaacg cgcggtagcc gcttaccaat tccttcaatt
     2101 gcggcagttc tgcctgccgg cgcgagctga cctcggcaag tatcctgctc aggcgctgcc
     2161 attgcgcggc gcgcccctgc aaccaggccg tcagcgcggc ctcaggccgg cttgccacgg
     2221 gactcgtccc tgagcttgcg caaccgcttg cgcagctcgg actcggtacc gccctgcgcc
     2281 accggctcgc cgatgcgggc caggaatttt tccgcgaggc cacggcgcag cggcgcatcg
     2341 agcccgcgcc agcgctcgag cagatcgtcc agcaattccc gatccgcggg cgacagccgc
     2401 gaggaagaaa agccgatcac gccgtcgaaa tcgaggctgc gtttgccagt gtcgtagacc
     2461 agcagcgtgc cggcggcaag atcgccgatg cgcacctggc gcgcggtgaa catcgccacg
     2521 accaggccta gcacgtagaa cgcgggcagc gaatccacca gcctgaagac gttgcggatg
     2581 atcagcgcgc cggcatcggg ggtgagtgcg cgctcggtga cgatgcgtat cccggccatg
     2641 cgctttcccg gcgtacggcc ctgcatcacg atttcgagca gcgggtggta gagaaaatag
     2701 atcgcggccg ccggcagcaa cgccgcgtac aaggccacct tgccggcctt cagcaaaggc
     2761 gagaagaaat cctccgctgg cgacagcagg ccgaccagca ccgcgaccag cgccagccag
     2821 gccagcgcca gcagaaagcg gaggtgccag tcgatcagga aagcgtagct gcggctgccg
     2881 atgccggcaa ctggcagctc gacttcgacc gcgtcggctg cttgcacaac gagtttgtct
     2941 tccacgaggg ccttctccga tcgcgctaac gcgcgccgaa tcccagtaat tccgcggcgt
     3001 gcttgcgcgt ggtctcggta atcttcacgc cgccgagcat gcgtgcaatt tcctcgaccc
     3061 gggaggcacg gtccagcacc gctacctggc tcgccacctt gccgtcggcc gccgacttgc
     3121 tcacctgcca ctgctggttg ccggccgccg ccacctgcgg caggtgggtg acgcacatca
     3181 cctggtgacg gctgccgagt tccttgagca tcctgccgac gatttcggcg acgcgcccgc
     3241 cgatgccggc atccacttcg tcgaaaatca gcgtcggcac cgcggccacc tggctggtca
     3301 cggtctggat cgccaggctt atgcgcgaga gctcgccgcc cgaggccacc ttcgccagcg
     3361 ccagcggggt ggcgccggca tggccggcaa ccaggaactc gatctgctcc agtccgtgcg
     3421 cgttgccttc gctcagggga tggagtgcaa tctcgaatgc gccgccgccc atcgccaggc
     3481 tctgcatcga cgcggtgact ctcttggcaa aatccgccgc tgccttcctg cgcgccgcag
     3541 agagcttcct ggcgcgctcc tgccagtgcg ccgaggccag attctcacgt tggcgcagcg
     3601 cttccgggtc gaacccggtt tccagctcgg tcagccgcgc cttcattttt gccagacgct
     3661 cgggcatctc aagcggattg accctgaact tgcgcgacgc cgtgtgcacc gcatcgagcc
     3721 gggtttccac ctcgcgcaac cgctgcggat ccagatccag gcggctctga taatggcgca
     3781 ggccgtaaac cgcctcctgc aactggatct gcgccggctc gagcgcagcg agaatttcct
     3841 tcagctgcgg gtcgaaatcc gtcatgccgt cgaggcgcga attcaccgaa gcgaccgccg
     3901 ccagcgtcga catctcgccc tcggacagca cgtcgaggct gaattgcacc gcttcgatca
     3961 ggctggcggc atgtgccagc cgcttgtgct ccgcctgcag ctcctcccat tcctcggccc
     4021 ggaaaccaag cgcatccagg tccttcacct gccactgcag ttgttcgcgc tcggcttcca
     4081 gcgcctgcgc atcggtctcc agcgccaggc gccggtggcg gatgtcctgc caggcgcgga
     4141 atgcctccgc aacttcatac gccagatcgg cggcgccggc gtggccgtcc agcaggtcgc
     4201 gctgcgcggg tgggcgcaac agcgactgat gcgcgtgctg gccatggata tccaccaggc
     4261 gttcgccgat ctccctgagc tgctgcaccg tggccggacg gccgttgacg tacgcgcgcg
     4321 agcggcccga agactcgatc acccggcgca gcaggcacac gccgttatcg ccgctgagat
     4381 cgttttccgt caggtaatcc gaaacggcgg gcgcttctcc ggtcgagaat tccgcgctca
     4441 cttcggcctt gtcttttcct tctcgcacct gtcccgactc ggcgcggctg cccagcaccg
     4501 tggccagcgc gtcgatcagg atggacttgc cggcaccggt ctctccggtc agcgcggtga
     4561 agccgggctg aaattcgagg tcgagcttgt cgacgatgac gaagtcgcgg atgctgaggc
     4621 tgcgcaacat ggctgcagaa aaaccggcgg cgatgacgga tcggcgttac agggtttcgc
     4681 tccaatggag tttttcccgg agcatgtgat agtagttgtg tcccagcgga tggagcaggc
     4741 ggatcgcgtg tgggcaacgg cgcacgatga ccttgtcgcg ctcctggagt tcgaagtgcg
     4801 aatggctgtc gaagtgcgcg cgggcatccg ccgccttgta aatcaggatt tcgatcaccg
     4861 actcgctgct taccacgatc gggcggttcg acagcgtgtg cggacagacc gggaccaggc
     4921 cgatgacgtt caggctggga tgcagaatcg gcccgccgga agaaagcgcg tacgcggtcg
     4981 agccggtggg agaggccacg atcagcccgt ccgagcgcag gttgtagacg aactgcccgt
     5041 cgatgtgcac ctcgatctcg atcaggctgc cctccgcgcc cttgttcacc gacacgtcgt
     5101 tgaatgccag cacgtccagc acctggcgtt cgccgctcaa caccgcggcc tgcagcagca
     5161 tgcgcgtttc ggtgatgaag ctgccgtcga ggatggagcc gatggcctcg aacatagtgt
     5221 ccatcgacag atcggtgaga aagccgagcc gcccctggtt gatccctacc agcgccacgt
     5281 tgtaaggcgc aaaggtgcgg gcgatgttga gcatggtgcc gtcgccgccg atgacgatgg
     5341 caagttcggc ggtacggccg acgtcttcga gcggcagcac ggcgtgattg ttcttgccga
     5401 tgtgcgcggc cgtcagcgga tcgagcacga tcctcacgcc ccgtcctttc agaaaatccc
     5461 ccagccgcag gagcggctcg gcgatctcgg ggctcttgta cttgccgatc agggcgatgg
     5521 tcttgaatgc gctcgacatg cctcgaatta aagcacattt ccccccgaac cgccggttcc
     5581 ggaaacactc caacgaaggc tacttcgagc ccgaaaagct ttgtagaata gccacatggc
     5641 aacgaacgat tccacgccta gcgaacgcgc gcagatcctg ctgaaattgc tggtcgagcg
     5701 ctacattacc gacgggcagc cggtcggctc gcgggcgctc gccaagtttt ccggactcga
     5761 cctgagcccg gcgagcatcc gcaacatcat ggcagacctc gaagaactgg gattcatcgc
     5821 cagcccccac acatccgccg gccgcatacc gacgccgcgc ggttaccgct tcttcgtcga
     5881 cacgctgctc aaagtcaggc cgctcgacca gatggagatc gaccagctgg aaggccagtt
     5941 gcaacccgat cacacccaga agctggtgtc gcaggcctcg cacctgcttt ccgatctcac
     6001 ccgcttcgcc ggcgtggtga tgacgcccaa gcgcaggagc gccgcattcc gccacatcga
     6061 gtttctgcgc ctgtcggaaa agcgcatcct gctgatcatt gtcgccccgg acggcgacgt
     6121 gcaaaaccgc atcatcctca ccgatcgcgc atacgagcct tcggagctta ccgaggccgc
     6181 caacttcctg aaccagaact tcgccgggtt gacgttcgac gagatcaagg cgcgcattca
     6241 tgatgaactg cgcaagctgc gcgaggacat gacgcggctc atgacccaag cgctcgaggc
     6301 cagcaccgag gtaatgaacc agaactcgga ggattacgtg ctttccgggg aacacaacct
     6361 gctgcacgtg caggatctgt cttcgaacat ggcgagcctg agacggctgt tcgacatgtt
     6421 cgagcagaag accagcctcc tgcagttgct ggacatcagc agccgcgcgc acggagtgca
     6481 gattttcatc ggcggcgaat ccggcctggt tccgctggac gagtgcagcg tggtgaccgc
     6541 gccctacgaa gtcgacggcc aggtggtcgg cacggtgggc gtggtcggcc ccacccgcat
     6601 ggcctacgag cgcgtgatcc cgatcgtgga catcaccgcc aaactgctct cgagcgcgct
     6661 ctcacaacat tgaattttgt tgaggcagga tgcccaagat aaaactttgg ggcattgcgc
     6721 gcggtactca cgcaacctga cgcctctcgc cttgcccctc gccccctaat gtcctttctt
     6781 cccgaaccca ggttccagca cggcacgatt gccaaggccg gaatcctgct gatcaacctc
     6841 gggacgccca ccgcaccgac cgccagggcg ctaaagccgt atctcaaaca attcctgtcc
     6901 gatccgcgcg tggtcgagat accgaggctg gtctggtggc cgatcctcaa cggcatcatc
     6961 ctcaataccc ggcccaagca gtcggccgcg aagtacgcaa agatctggac cagagacggt
     7021 tcaccgctga gggttcacac cgaaaagcag gcgacgctgt tgaaaggata tctcggcgag
     7081 cgcgtgaaga ccccgcacag catcgcctgg gcgatgcgct acggcgaacc gtcgatcggt
     7141 accgtcctgc gcgaactgcg cggccaggga tgcgaccgca tagtggtgct gccgctgtac
     7201 ccgcagtatg cggctagcac caccgcgagc gccttcgacg cggtgttcga tgcagtcaag
     7261 agaatgcgca acgcgccggc gctacggctg gtcaagcact accacgacca ccccggctat
     7321 atcaccgcgc tggccaagga catcaatgat tactggatgg ctaacggccg gccggacaag
     7381 ctggtgatga gctttcacgg cgtgccgcgc ttcacgctgg atcgtggcga tccctaccac
     7441 tgtgagtgcc aagtgaccgg ccggttgctg gcccaggaac tggggctcaa gcccgaacaa
     7501 tatatggtga ctttccagtc gcgcttcggc cgcagcgaat ggctcaagcc ttacaccatc
     7561 gataccatcg gaacgctggg caagcgcaag acttctcgag tggacgtggt gtgccctgga
     7621 ttcgtgtccg attgcctgga aaccctggag gagatcggca tcgagaacaa ggccgccttc
     7681 ctcaaggcag ggggcggaga attccactat atcccctgcc tgaaccagcg gcacgaatgg
     7741 atccaggcgc tggcccgcat cgccgcggaa aacctgcacg gctgggcgtc caccgactgg
     7801 gatcaggccc cggcgctgcg cgccgctgcg gagagccgca cgcgcgcggt tgcgatgggc
     7861 gcaaagaact agctcatcag gttatggcag cgtcgcgcct tgattgcggc caccggtaaa
     7921 agcggctggc agtatagtca tccagcgttc gcgcccgaaa ccataaagta agcccttcct
     7981 cgcacaatga actcctgaaa tcaggcggtg tggggccgtg ttcagcgtcg ggccagggtt
     8041 gaaatagcgc tatcggaccc caacatgact ggcagtttgg acccaggagg agctgatgca
     8101 ggaccaggaa aaattggcgg ccgtgccgca gaatgacagt ggcacagcct cgctggcggc
     8161 ggaggacgca ggatatgacg ccccggcagt cgagaccatg ccgggtatgg aagagttgct
     8221 gaagaaggcg gagcttgcgg cccaggaaca ccacgatgcc tggttgcgcg ccaaggccga
     8281 gaccgaaaat gtgcgcaggc gcacgcagac cgacattgcc aacgcccaca agtacgccct
     8341 tgagggtttc gccagcgaac tgcttgcggt acgcgacagc ctggaggcgg cgctcgctcc
     8401 cggcaacgcc accccggatg ggctgagaag cggcgttgag ttgaccctga aacagttgac
     8461 caacgcgttc gagaaatttt caattaaaga aatcaatccg ttaggggaaa aattcgaccc
     8521 ccacaaacac cagggaatcg gcctggtgga agccgatgcc gatcccaaca ccgtggtcag
     8581 cgtgctgcag aaaggctaca gcctgaacga ccgggtgatc cgcccggcac tagtcaccat
     8641 cgccaaatcg cgggcctctg cttgattttc aaaacggaag accaaatctt cgatatcaaa
     8701 ggcattctca ctttcgaaca aaggaaagac catgggaaag attatcggta ttgacctcgg
     8761 caccacgaat tcatgcgtgg ccgtgatgga gaacggccag cccaaggtga tcgagaactc
     8821 cgaaggcgcg cgcaccacgc cctcgatcgt cgcctacatg gaagacggcg aaatcctggt
     8881 cggcgcacca gccaagcgcc aggcgatcac caaccccaag aacacgcttt tcgcggtcaa
     8941 gcgcctgatc ggccgccgct tcatcgaacc ggaagtacag aaggacatcg acctgatgcc
     9001 ctacaagatc gtccagaaca gcaacggcga cgcctggatc gaggtacgcg ggaaaaagat
     9061 ctcgccgccc gaggtctctg cacaggtgct ggtgaagatg aagaagaccg ccgaggacta
     9121 tctcggcgag ccggtcaccg aggcagtgat cacggtgccc gcctacttca acgattcgca
     9181 acgccaggcc accaaggacg ccggccgcat cgccggtctg gacgtcaagc gcatcatcaa
     9241 cgagccgacc gcggcggcgc tcgccttcgg catggacaag aaggaaggcg accgcaagat
     9301 cgcggtatat gacctgggcg gcggcacgtt cgacatctcc atcatcgaaa tcgccgtggt
     9361 ggacaaggaa caccagttcg aggtgctctc gaccaatggc gacaccttcc tcggcggcga
     9421 ggacttcgac cagcggctga tggactatct cgccgatgag ttcaagaagg aacagggaat
     9481 cgacctgcgc aaggacgtgc tggcgctgca gcgtctcaag gaagcggccg agaaggccaa
     9541 gatcgagttg tcgtccagcc agcagaccga gatcaacctg ccgtacatca ccgccgacgc
     9601 ctcgggaccc aagcacctgg cgatcaagat cacccgcgcc aagttcgaaa gcctggtcga
     9661 tgagctgatc cagaagacgg tggaaccgtg ccgcaccgcc atcaaggatg ccggcgtgaa
     9721 gctctccgac atctccgacg tgatcctggt gggcggcatg acccgcatgc cgaaggtcca
     9781 ggagaaggtg aaggaaattt tcggcaagga accgcgcaag gacgtgaatc cagatgaagc
     9841 agtcgcgatc ggcgcggcga ttcaggctgg cgtgttgcag ggcgacgtca aggatgtcct
     9901 cctgctcgac gtcacgccgc tcagcctggg catcgaaacg ctgggtgggg tgatgaccaa
     9961 gctgatccag aagaacacca cgatcccgac caaggcgcag caggtgttct cgaccgccga
    10021 cgacaaccag acggcggtaa cgatccatgt gctgcagggc gagcgcgaaa tggcctccgg
    10081 caacaagagc ctcggccagt tcaacctgtc cgacatcccg ccggcgccgc gtggactgcc
    10141 gcagatcgaa gtcacattcg acatcgacgc caacggcatc ctgcacgtct cggccaagga
    10201 caaggccacc ggcaaggaaa acaagatcaa gatccaggcc aattcgggtc tcaaggaaga
    10261 cgagatccag cgcatggtga aggacgccga agcgcacgcc gacgaggacc gcaaggcccg
    10321 cgagctggtg gaagcgcgca accagtgcga cgcgctcatc cacgcggtga agaaatcgct
    10381 caccgagcat ggcgacaagg tcaccgcgga cgagaagtcc ggcatcgaag cggcgatcaa
    10441 ggcggcggaa gaagccatca agagcggcga caaggacgtg atcgaggcca aatccaaggc
    10501 cctggccgaa gcgtcgcaca agctggcgga gaagatgtac tcccagcagc aggcgcaagg
    10561 cggcggcgcc cagccccagt cagaatccgg cggcgccaag cccgaggacg gcaacgtggt
    10621 ggatgcggaa tacgaggaag tgaaggacca gaagaaatcg gcgtgataag gtaaggctta
    10681 acgcgtgagg ggtgaggcgg atttccggct caccccttgc cttgtctaaa aaatagagcg
    10741 acggcccagc caagaaggca ctgaatgtcg aaacgcgact attacgaggt actcggcgtc
    10801 aatcgcgacg cttccgaaga ggaaatcaaa aaggcctacc ggaagctggc gatgaagcac
    10861 catccggatc gcaatccgga caatccgaaa tccgaggggc tgttcaagga ggcgaaggaa
    10921 gcctacgaag tgctatccga cgccggcaag cgcggggcct acgatcaata cggccacgcc
    10981 ggagtcgatc ctcgggccgg aatgggagcg ggagcggccg gtgcgggctt cggtggtttc
    11041 gccgatgctt tcggcgatat cttcggcgac atcttcggtg cggggcgcca gcgctccaac
    11101 gtctaccgtg gcgccgacct gcgctacaac ctggaaatct cgctggaaga agccgcccgc
    11161 ggcaccgaga cccgggtgcg catcccgacc atggaggagt gcgtgacctg tggcggttcg
    11221 ggcgcaaaac ccggcacgca gccgatgacc tgtccgactt gcggcggtca gggacaggtg
    11281 cgcataaccc agggattctt ctcgatccag cagacttgcc cgaaatgcca tggcagcggc
    11341 aaagtcgtgc agagtccctg cgctacgtgt aatggcgcgg gccgggtcaa gcgccagaag
    11401 acgctgtcgg tcaagattcc cgccggcgtg gacgagggcg accgcatccg cctttccggg
    11461 gaaggcgaag ccggggtcaa cggcggtcca tcaggcgatc tgtacgtggt gatccacatc
    11521 cgtgcgcacg aggtgttcag ccgcgagggc aacgacctgc actgcgagat gccgatcagc
    11581 ctcaccaagg cggcgctggg cggggagatc gagattccga cgctggacgg ttacgccaag
    11641 atcaagattc ctctggagac ccagacgggg aaagtattcc gtttgcgcgg caagggcatc
    11701 aaaggcgtgc gcagcaacgc ctacggcgac ctgctttgcc acgtggtggt ggaaacgccg
    11761 gtgaatctca cgccgcggca gaaggaattg cttcaggaac tcgaagagat caacgatcgc
    11821 gacaacggcc gccacaaccc gcgcgccaaa agctggatgg accgcgtgag ggaattcttc
    11881 gccgaataga agtcgcgact tcaagaaaag ggccgccggt gcggcccttt ttcatgctca
    11941 cgacaccgcg ttacttgcgg cgccaggtcg tgcctgcggg cccgtcctcg agcacgatcc
    12001 ctgccgcgag cagttggtcg cggatgcggt cggactcggc gtagttcctg gccttctttg
    12061 cttgcgcgcg cgccgcgatc atttcttcga cccgtgccac ggtccagccg gcgggtagat
    12121 cgccgcgcaa gaactcgtcg ggatcgcgtt gcagcaggcc caacactgcg ccgagcgatt
    12181 tgagcagcgc ggccgtgttc gaggaccggc tgcgattgac ttcgttagca agatcgaaca
    12241 gcacggcaac cgcttccggg gtattgaaat cgtcgttcat cgccgcttgg aaacgcgccg
    12301 cctgcggctc gttccagtcc acatcgccgg cctgcgccgg caccgctttg agcgcagtat
    12361 aaagccgggt cagcgcctgc ctggcgtcat cgaggtgctg gtcggaatag ttcagcggac
    12421 tgcgatagtg cgcgcgtacg atgaagaagc gcaccacttc tgcgtcgtac ttcgtcagga
    12481 tttcgcgcac ggtgaagaaa ttaccgagcg acttcgacat cttctcgttg tctacgcgca
    12541 cgaagccgtt gtgcatccag tagttcacga aggcgtggcc gtgcgcgcct tccgactgag
    12601 cgatctcgtt ctcgtggtgc ggaaactgca ggtcctggcc gccgccgtgg atgtcgaagt
    12661 ggctgccgag gtag
//
LOCUS       DASQOP010000129        20904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2471703, whole genome shotgun sequence.
ACCESSION   DASQOP010000129 DASQOP010000000
VERSION     DASQOP010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 20904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20904
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2471703"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VE030_05205"
     CDS             complement(<1..506)
                     /locus_tag="VE030_05205"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HZF22849.1"
                     /translation="MTLDDLRRLDPRDIGSWPVAPKLGALLILLLFIVFAGYWFDWRN
                     QLEELAQARDQEQELRTAFLDKQKQAVNLEAYRKQLAEIEESFGEMLKQLPNKSEMEA
                     LLTDINQAGLGRGLQFELFRPAQKENMSEFYAELPITIKLTGKYHDIGAFASDVSQLS
                     RIVTLNDIA"
     gene            complement(512..1129)
                     /locus_tag="VE030_05210"
     CDS             complement(512..1129)
                     /locus_tag="VE030_05210"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HZF22850.1"
                     /translation="MIPINLLPHRAERRKAQTRQFAVLAVMTVVLAGAIVGAVHTLFA
                     SRIENQLSRNKYLQTEIAALDKQIDEIKKLKEQTQALLARKRVVETLQTNRTETVRLL
                     DQMVRQLPDGVHLKTVKQTGQKVNVVGYATSNARVSTLMRNLEASPWLEAPSLVEVKA
                     TRLGKDNIILNEFILNVTISRPKEEAAGAKPAAPATPRAATAPKA"
     gene            complement(1126..2232)
                     /locus_tag="VE030_05215"
     CDS             complement(1126..2232)
                     /locus_tag="VE030_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HZF22851.1"
                     /translation="MLKGKFEFNADFLGGLLKAKSPPLVGVDVSSSSVKMVELTGSGR
                     GRYRLERYSIEPLPKDAVVDGNIMNLEVVGDALRRGWKRMGTRVKNLAMALPSAAVIT
                     KKIVVPAGQTEDQLEAQVETEANQYIPFALEEVNLDFQILGPAPNSSEDVEVLIAASR
                     KEKVDDRVAVAESVGLKAVVMDIDSFASQNAFELMQGQLPEGGHDQAIALVDIGASMM
                     NINVLRNNQSVYMREQPFGGNQLTQEIMRHYNLSAEEAEAAKRNGGLPDTYDSEVLAP
                     FMDTLALEVARALQFFFTSTQYNQVHHIFLSGGSATIPGLPEVVAERTQVNTLVANPF
                     AGMEVSSKVRQAQLAVDSPALLVACGLALRRFDE"
     gene            2391..4721
                     /locus_tag="VE030_05220"
     CDS             2391..4721
                     /locus_tag="VE030_05220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HZF22852.1"
                     /translation="MSSRWWFYGLFGLAAIAAAIVLVVGYAMVVAYPNLPSLEALTSY
                     QPKIPLQIFSVEGDLIGEFGEEKRAFVKIEQTPDILRKAIIAAEDERFYEHGGVDYIG
                     VVRATLSNFAAASARQGASTITMQVARNFFLSTEKTFSRKFNEALLAFKIEHSLTKDE
                     ILQLYINQIYLGQRAYGFGAAALVYFGKPLDQLNLGEIAMLAGLPKAPSRYNPVINMN
                     RAKQRQRYVLRRMLELNHIDAEQFKQAEAQPIIVTRQVQEFATRADHFAEMVRQAIYD
                     AYKEEAYTRGFRVYTTLSKVHQDAAYRALRQGVLEYDERQNYRGAEGYFDLPKEEIGE
                     EALEDSLQDELESDDIYPAIALEVDAKAVKAYRKGGEFVTITGDGLKFASRMIGNRAP
                     AKLRLRRGALIRAQPDGKGGWKIVQLPQVEAALVSVNTNDGAIRAMVGGFDFYRNKYN
                     HVTQARRQPGSSFKPFIYSAALEKGFTPATIINDAPLTIDASETGSVNWEPRNFDGTF
                     DGPIRMRTALTRSKNLVSIRILQAITPQYAQDYISRFGFDPKQHPPYLTMALGAGSTT
                     PLEMVMGYSVFANGGFRVTPYFIERIEDAQGEILGKANPIQVGKGAERVIDSRNAFIM
                     ANMMQDVIRGGTGARAMALGRVDLAGKTGTTNEQLDAWFAGFQRNLATVVWIGYDIPR
                     TLGDHETGAVAALPIWMSYMGTVLKGQPEELWVAPQGVVAVAINPQTGLRDRHAATPM
                     IEYFYHENVPPEQESSPEDQLGDSAKPPEEVRDQLY"
     gene            4728..5363
                     /locus_tag="VE030_05225"
     CDS             4728..5363
                     /locus_tag="VE030_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--alanine ligase"
                     /protein_id="HZF22853.1"
                     /translation="MSRENGNNRQMRERIAQLAARLMAVDGIDDFALAKRKAARQAGA
                     PDTRNLPNNEEVEQALRAYQQLYQADEQQARLRRLRQNAREMMQLLAQFDPHLSGSVL
                     SGSAGKYSDINIHLFTDSVKDVEMFLLNRQIPYRSRERRVYIGGEQRSVPNFTVSTDD
                     ADFDITVFAPRDLRYQLRTTAEGKPFDRARIDWLNSVLESARTAGAATNLL"
     gene            complement(5350..6354)
                     /gene="hemB"
                     /locus_tag="VE030_05230"
     CDS             complement(5350..6354)
                     /gene="hemB"
                     /locus_tag="VE030_05230"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HZF22854.1"
                     /translation="MHVLGKFPVRRMRRLRRDDFSRRMVREHRLSSDDFIYPVFVIDG
                     QHRSEPVPSMPGVERLTVDALLRQAERCVELGVPAIALFPVVDNKLRTADAREAFNPD
                     GLAPRSIRALKSRFPQLGVITDIALDPYTSHGQDGLIDDSGYVLNDETIEALVKQALT
                     HAEAGVDIVAPSDMMDGRVRALRRALDESGHIYTRILAYAAKYASSFYGPFRDAVGSA
                     ANLGRGDKYTYQIDPANSDEALWEVGMDLEEGADMVMVKPGMPYLDIVRRVKDEFGAP
                     TFVYQVSGEYAMLQAAGVNGWIDRRKCVLESLLAFKRAGADGILTYFALEAAAWLKED
                     "
     gene            complement(6609..7214)
                     /gene="yihA"
                     /locus_tag="VE030_05235"
     CDS             complement(6609..7214)
                     /gene="yihA"
                     /locus_tag="VE030_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HZF22855.1"
                     /translation="MSRTRLFNDVAFFATAAHERDLPASSGVEVAFAGRSNAGKSSAL
                     NALARRHRLAFVSKTPGRTQQINFFSLGVGRFLVDLPGYGYARVPGATKRSWQELLSG
                     YLQARGQLRGLVVVMDARHPLTPLDRQLLDWFAPTGRPVHVLLTKADKLSKQEASRQL
                     RIVGERLAAEYHGSSVQLFSATTKQGVDEAETLLADWFDAG"
     gene            7290..7901
                     /locus_tag="VE030_05240"
     CDS             7290..7901
                     /locus_tag="VE030_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZF22856.1"
                     /translation="MNRLIAAAVVALISVTSAVAADYGDPAKAQSIANQICAACHGAD
                     GNSPLPMNPSLAGQHPEYLFKQLNEFKSGNRNNAVMMGMVAGLSAEDMRNLSAYYAAQ
                     KLREAAAKDKDLVAHGRKLFRGGNLATGVAACAGCHSPNGAGIPSQYPRLAGQHPEYV
                     VAQLKAFRAGERANDTNNMMRAVAARLTDKEIAAVAEYLSGLR"
     gene            complement(7914..9203)
                     /gene="hemL"
                     /locus_tag="VE030_05245"
     CDS             complement(7914..9203)
                     /gene="hemL"
                     /locus_tag="VE030_05245"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HZF22857.1"
                     /translation="MKTRNEILFEKSQTVIPGGVNSPVRAFRSVGGTPLFVSRGEGAC
                     VWDADGRRYVDYVGSWGPLVLGHAHPQVVEAVREAASRGLSFGAPTEAELEMAELITR
                     LLPSMQQVRLVNSGTEATMSAIRLARGFTGRAKIVKFEGCYHGHADSLLVKAGSGSLT
                     FGQPSSAGVPAEITTHTLVLDYNDAGMLERAFGAQGDEIAAVIVEAVAGNMNLVKPRP
                     EFLRALRDLCTKHGAVLIFDEVMTGFRVGLQGAQGLYRITPDLTTLGKVIGGGMPVGA
                     FGGRRDIMQFIAPLGPVYQAGTLSGNPVAVAAGLATLKLVQAPGFFDRLGNAAAALVQ
                     GLQKAGRDAGLAFSAQSVGGMFGMYFRASSPESYAQVMECDKNAFNRFFHAMLVQGVY
                     LAPSAFEAGFVSAAHGDAEISTTIAAASAAFAKVAAA"
     gene            complement(9214..9846)
                     /gene="thiE"
                     /locus_tag="VE030_05250"
     CDS             complement(9214..9846)
                     /gene="thiE"
                     /locus_tag="VE030_05250"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HZF22858.1"
                     /translation="MRKTVSGLYAVTPGLADSALLTSKVEAALRGGAHVVQYRNKTAD
                     ERLRREQALRIARLCREVGACFIVNDSIELAREVAADGVHLGKDDDGVGAARALLGPG
                     KLIGVSCYNQLWRARDAVVQGADYIAFGSFFPSPTKPGAVTAGPDLLRAARKFSRPIV
                     AIGGITPDNASGLIEAGADAVAVVAAVFDAHDVESAARRIAGLFAERSES"
     gene            complement(9830..10663)
                     /locus_tag="VE030_05255"
     CDS             complement(9830..10663)
                     /locus_tag="VE030_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HZF22859.1"
                     /translation="MPDSLPIVLVFAATDPSGGAGVQADILTLSSMGCHPLSVITAVT
                     IQDTMGVEDVLPIDAEWVADQARCVLEDVPVAVFKVGLLGSVEAIAAIAEVVSDYPDI
                     PLVLDPVLASGRGDELASEDMISAMRELLIPQTTVITPNSLEARRLAQDDSNENDDPD
                     LNECARRLLNLGCEFVLVTGTHENTAQVVNTLHDQQGVVRTDSWQRLPGIFHGSGCTL
                     ASAVAATIANGLGVPEAVRDAQEYTWQTLKFAYRTGMGQLIPDRLFWAREEEPEDAQD
                     G"
     gene            10739..10927
                     /locus_tag="VE030_05260"
     CDS             10739..10927
                     /locus_tag="VE030_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HZF22860.1"
                     /translation="MAAGEKEEYKTYMCLICGFIYDEATGLPEEGLSAGTRWEDVPVN
                     WVCPECGARKEDFELVEI"
     gene            11031..11417
                     /gene="gloA"
                     /locus_tag="VE030_05265"
     CDS             11031..11417
                     /gene="gloA"
                     /locus_tag="VE030_05265"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019247766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HZF22861.1"
                     /translation="MRILHTMLRVGDLKHSIDFYTKVLGMKVLRQKDYPDGRFTLAFI
                     GYGDEDKEAVLELTHNWDTAKYDLGNGYGHVALEVDDAYKACEEVKKRGGKVTRDAGP
                     MKHGTTVIAFVEDPDGYKIEFIQRKR"
     gene            complement(11414..12196)
                     /gene="rsmA"
                     /locus_tag="VE030_05270"
     CDS             complement(11414..12196)
                     /gene="rsmA"
                     /locus_tag="VE030_05270"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HZF22862.1"
                     /translation="MQATHTPRKRFSQNFLEDRGIVGQIVAAIDPRREDRIIEIGPGP
                     GALTAPLLDRVARLDVIEIDRDIVRALRARFPPESLQVHQGDVLEFDFSAFGAGIRVV
                     GNLPYNISTPILFHLNAQVALLRDCHFMLQREVVERMAAEPGSPEYGRLSVMLQYRWV
                     IESLFDVPPTAFHPQPKVWSSVARMTPYASPPHRASNESLFADVVTRAFGQRRKTLRN
                     ALRELLEAGELEQLGIEPGARGETLGVPAFVLIANYLEARRS"
     gene            complement(12237..13541)
                     /locus_tag="VE030_05275"
     CDS             complement(12237..13541)
                     /locus_tag="VE030_05275"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZF22863.1"
                     /translation="MRILALFLALLGIAPAGAAQGERTHGDPALLDRVVAVVNNEVIT
                     RLDLDEQVRVALQQLRRQNTPLPAQDVLERQLLERLVTARVLAQTAKETGLRVDDAQL
                     QRSLERIAQENKLTPDAFRKALESDGVNFNRFRDELRNEILITRLKEREVDSKILITD
                     AEIDNYLKTQQNLGGKDDEYSLAHILVLVPEQASPEQIQAKRSAAEKALAQLRGGADF
                     RQVSAGVSDAQNALEGGPLGWRPASRLPQIFVDVVRTMKVGDLSAVLRSANGFHILKL
                     LDKRGNETPVIVQQTRTRHILVRLNEVVSEADAKQRLFHLKERIDNGADFAELARLQS
                     EDASSSRGGDLGWLSPGDTVPEFERAMNALEPGQVSEPVQSSFGWHLIQVLERRNEDM
                     SKERQRLLARQAIRARKSDEAYQEWVRQQRDRAYVELRLEER"
     gene            complement(13615..15831)
                     /locus_tag="VE030_05280"
     CDS             complement(13615..15831)
                     /locus_tag="VE030_05280"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HZF22864.1"
                     /translation="MHRPRFASPIVLLICFLAPGAGADEPGLKLKIQPALIPYSEGSN
                     QPTPMFLEADRLQGHQERELEAEGNVSLRKRGAAVFADYLYYAFPERELTVTGHVRFE
                     KEGDVVTGEKLFYNLDSESGYIEKPAYNFQQFRARGSADRMVMESRTKIRADKATYTN
                     CDVGDDDWFLRVDKLDLDRQRDVGVAHNATVVFKGLPVLYSPYLDFSLSGRRKTGLLP
                     PTIGSTGQSGFEVTQPFYWNIAPNRDATIAPRILAKRGVLLNTELRYLEPSLSGEVRT
                     EYLPNDRQKQESRYGYSWQHQELFGRGISGGFNLQGVSDDTFFTDLSDKIAVTSQTNL
                     PREGNLLYDGDWWNLNARVQRFQTLQDPLAPVTPPYARAPQLTLNVNRQTDYYLDLGL
                     QGEYVDFDHPFLLNGRRQILYPSISVPLQTSYFYLTPKLGYHNTRYSFEDPNLPDETR
                     GLPIYSLDSAVTLERNARLWGRDFIQTLEPRLYYVYIPFRPQDQLPVFDTAVADFNLA
                     QIFTENQFTGGDRINDANQLTAALSSRLINPGSGEEQLRFVLGQRYYFKEQQVSLSTS
                     RRDFNRSDILAAMTGRITPNWVADVGLQYSAITSRTERSNVALRYQPAIGKMMNFGYR
                     FTRDTLEQVDLSSQWPIAGRWTGLARWNYTLRDKRLLEGLAGVEYNAGCWAARFVVHR
                     FVSATQEYVNAMFFQVELNGVSKIGSNPLELLRQNVTGYTKTNEPPPAERNPFPAY"
     gene            15897..16889
                     /locus_tag="VE030_05285"
     CDS             15897..16889
                     /locus_tag="VE030_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HZF22865.1"
                     /translation="MDRYASLQDWIARQFPGITFTLSPASVDASFRRYFRVTFPDSTL
                     IAMDAPPAQEDCRPFVRVAALMAEAKVHVPKVLAHDLARGFLLLSDLGATTYLAALNP
                     GNADQLFGDAIDALIRWQLASREGVLPAYDKTSLKRELDLFPEWYLDRHLGIKLSSLQ
                     RESLEKIFSLILASNLAQPKVYVHRDFMPRNLMVSNPNPGVLDFQDAVFGPIGYDVAS
                     LFKDAFLSWEEERVLDWTVRYWEKAKRAGLPVDADFGVFYRDVEWMGLQRHIKVLGIF
                     ARICHRDNKSGYVEDAPRFLDYVRVVARRYAALAPLLRLLDQFQDREPAVGHTF"
     gene            16919..17602
                     /locus_tag="VE030_05290"
     CDS             16919..17602
                     /locus_tag="VE030_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HZF22866.1"
                     /translation="MILAAGRGNRMRPLTDLTPKPLLAVGGKPLLFRHLEKLAHAGFG
                     TVTINHAHLGAQIEAAVGDGGRWNLQVRYSAEAESLETAGGIRNALPLIGGRTFAVIN
                     GDVYCDYDYAGLAAAIERLADSQALAHLVLVDNPPHHPNGDFCLEAGLVRSSGWQPLT
                     FSGLGAYRAELFESLARGAKHALAPLLRSQLPQDNVTGEHYRGLWTDVGTPGRLAELN
                     ARLQPGART"
     gene            17599..18219
                     /locus_tag="VE030_05295"
     CDS             17599..18219
                     /locus_tag="VE030_05295"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HZF22867.1"
                     /translation="MIHIPEHFAEHDIARLHALVRAHNFATLVSAHQGEIQVSHLPFL
                     LDAGGKLLRAHMARANRQWRTFSPEREVVVIFHGPHHYVSPAWYASHPSVPTWNYAVV
                     HAQGRPRLIEDRERLAALLRELVTRHEAASPEPWKMDLPPDYQDKMLSAIVGFEIEIT
                     RLDGKFKLSQNRPESDRPLVIEALERLGSDDALGVAALMRENQGKL"
     gene            18283..19584
                     /locus_tag="VE030_05300"
     CDS             18283..19584
                     /locus_tag="VE030_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HZF22868.1"
                     /translation="MDNHRLQAQRRERLVSLIKQGVAVVRTAPERARNRDSTYPYRFD
                     SYFYYLSGFPEPEAVLVLIAGAKPKSILFCREKNPEREIWDGFRYGPDQAREIFAFDE
                     AHPISDLDELMPQLLADQPSVFCDVGSDGDWDSRVIGWINKVRAQVRSGVTAPAEICD
                     IRLFVDDMRLIKDPHELGTMRRAAAISTEAHRRAMRATRPGLREYEVEAEIVHEFRRR
                     GAQAPAYTPIVAGGANSCVLHYVQNDAILKDGDLLLIDAGCELDGYASDITRTFPVGG
                     KFAGPQRDIYEMVLAAQAAAIAEVKPGAEWENPHNAAVKVLAQGMVDLGLCKGTVNSA
                     IESGDYRRFFMHRTGHWLGLDVHDAGDYKRNGKWVELNPGMVLTVEPGCYVRPGEGVP
                     EKFWNIGVRIEDDALVTGNGCEIITTAAPKKIEDIESLMRA"
     gene            19605..20771
                     /locus_tag="VE030_05305"
     CDS             19605..20771
                     /locus_tag="VE030_05305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZF22869.1"
                     /translation="MSESEHDLVIVGGGPVGAALAIALSDASHDVLLLEARPELDTAS
                     DGRTLAMSYGSRLILARLGAWKRVEPATSIHSIHISQRGGFGRALLAAEDAGLPALGY
                     VVAHARLQKMLGEALASSRATLALGASVADIEFSADRVAIRFRRGDQEHCVGARLLVI
                     ADGGASLGKRAGATIKVRDYRQDAIVATVATSRPHRQRAFERFTADGPVALLPFEDRY
                     ALVWTAAPEAAARFTEMNDKSFLERLQLHFGDRAGRFLSVTARARFPLALRYAANPVL
                     PRSVLLGNAAQALHPIAGQGFNLGLRDAWELAELLARRDDRDPGSAACLSAFRSARRL
                     DRLGGIAITDSLVRIFSNDFVPLRAARGAGLALLDIAPPVKRAFMQPMIFGASL"
BASE COUNT         3959 a         6689 c         6334 g         3922 t
ORIGIN      
        1 gcgatgtcgt tcagggtcac gatccgcgac aattgggaaa cgtcgctcgc gaacgcgccg
       61 atatcgtggt atttcccggt gagtttgatg gtgatcggca gttccgcgta gaactccgac
      121 atgttttcct tctgagccgg acggaacagc tcgaactgta ggccgcgacc caacccagcc
      181 tgattgatgt cggtaagcag cgcttccatt tccgacttgt tgggcagttg cttgagcatc
      241 tccccgaagg attcctcgat ttccgcaagt tgcttgcggt aagcctcaag attcacggcc
      301 tgtttttgct tgtcgaggaa cgccgtacgc agctcctgct cttggtcgcg cgcctgcgcc
      361 agttcttcca gttggttcct ccagtcgaac cagtacccgg caaatacgat gaacagaagc
      421 aaaatgagca atgctcccag cttgggcgcc accggccagc tgccgatgtc cctcgggtcc
      481 agtcgcctca ggtcgtcgag cgtcatgatc gtcaggcttt cggcgcggtc gcagcgcgcg
      541 gcgtggctgg cgcagccggt ttggctcccg cagcctcttc cttgggccgc gagatggtca
      601 cgttgagaat gaattcgttg agaatgatgt tgtccttgcc cagcctggta gccttgactt
      661 caaccaggga cggggcttcc agccagggcg aggcttcgag attgcgcatc agcgtcgaca
      721 cccttgcatt ggaggtcgca tagccgacca cgttgacctt ctgcccggtc tgcttcaccg
      781 tcttcagatg cacgccgtcc ggcagttgcc tcaccatctg atcgagcagt cgcacggtct
      841 cggtgcggtt ggtctgcagg gtttccacca cgcgtttgcg cgccagcagc gcctgtgtct
      901 gctccttgag cttcttgatc tcgtcgatct gcttgtcgag cgctgcgatc tcggtttgca
      961 ggtatttgtt ccgactcagc tgattctcga tgcggctggc gaacagcgta tgaaccgcgc
     1021 cgacgatcgc acccgccaga accacggtca tgaccgccag cacagcgaac tggcgcgtct
     1081 gcgccttgcg ccgctccgcg cggtgcggca gtaggttgat tgggatcatt cgtcgaatct
     1141 ccgcagtgcg agtccgcagg caaccagcag cgcgggcgaa tccaccgcta gctgggcctg
     1201 tcgaactttg gaggaaacct ccattccagc gaacggattg gcgaccaggg tattgacctg
     1261 agtgcgctct gccacaactt ccggcagacc gggaatggtc gcggagccgc cggaaagaaa
     1321 gatgtggtgg acctgattgt actgggtgga ggtaaaaaag aactgaagtg ctctggcgac
     1381 ttcgagcgcc aaggtgtcca tgaacggagc caggacttcg gagtcatagg tatccggcag
     1441 cccgccgttg cgcttggcgg cctcggcttc ctcggccgag agattgtagt ggcgcatgat
     1501 ctcctgggtg agctgattgc ctccaaatgg ctgctcgcgc atgtagaccg attgattgtt
     1561 gcgcaacacg ttgatgttca tcatcgaggc gccgatatcc accagggcga tggcctgatc
     1621 gtggccaccc tcgggtagct ggccctgcat cagttcgaac gcgttctggg aggcaaagga
     1681 gtcgatatcc atcaccactg ccttgagacc gaccgactcg gccacggcca cgcggtcgtc
     1741 gactttctcc ttgcgcgagg cggcgatcag gacttccaca tcctcgctgc tgttcggggc
     1801 tgggccgaga atctggaagt cgaggttgac ttcctccagg gcaaacggaa tgtactggtt
     1861 ggcttcggtt tcgacttggg cttcgagctg gtcttcggtc tggcctgcgg gaacgacgat
     1921 cttcttggtg atgactgccg cggagggcag ggccatcgcc agattcttga cccgggtgcc
     1981 catacgcttc cagccgcgcc tgagcgcatc gcccaccact tcgaggttca tgatgttgcc
     2041 gtcaaccacc gcgtccttcg gcaacggctc gatggaatag cgctcgaggc gatagcggcc
     2101 cctgccggaa ccggtcaact cgaccatttt cacggacgag gaactgacgt cgacgccgac
     2161 aagaggaggc gacttggcct tcagcagacc gccgagaaag tccgcgttga actcgaattt
     2221 tcctttgagc attggccctt taggcgcgat acgtataatc tacattaaat gctagcaaca
     2281 actattttgc aagtaaagcg atttaacagc ccccttctgt cgggtctggg caacatctat
     2341 atataatgcc cgccgttccc cctgccggca ccccccctta gatctatccc atgtcctccc
     2401 gctggtggtt ttatggcctg ttcgggcttg ccgcgatcgc ggccgcgatc gttctggtgg
     2461 tcggatatgc catggtggtg gcctatccga acctgccttc cctcgaggca ctgacctcct
     2521 atcagcccaa gattccactg cagatcttca gcgtcgaggg cgacctgatc ggggagttcg
     2581 gcgaggaaaa gcgggccttc gtcaagatcg agcagacccc cgacatactg cgcaaggcaa
     2641 tcatcgcggc cgaggacgag cgcttttacg aacacggtgg ggtcgattac atcggtgtgg
     2701 tacgcgccac actgtccaat ttcgccgccg ccagtgcccg acagggcgcc agcaccatca
     2761 ccatgcaagt ggcacgaaac ttctttcttt ccaccgagaa gactttcagc cgcaagttca
     2821 atgaagcgct gctcgcgttc aagatcgagc acagcctgac caaagacgaa attctgcagc
     2881 tttatatcaa ccagatttac ctcggccagc gcgcctacgg cttcggcgcg gccgcgctgg
     2941 tgtacttcgg caaaccgctc gaccagctga accttggcga gatcgcgatg cttgccgggc
     3001 tgcctaaggc gccgtcgcgt tacaacccgg tgatcaacat gaaccgcgcc aagcagcggc
     3061 agcgctacgt gctgcgccgc atgctcgagc tcaatcatat cgacgccgag cagttcaagc
     3121 aggcggaggc ccagccgatc atcgtcaccc gccaagtcca ggagttcgcg acgcgggcgg
     3181 accacttcgc cgagatggtg cgtcaggcga tctacgacgc ctacaaggaa gaagcctata
     3241 cccgcggctt ccgggtctac accacgctgt ccaaggtgca ccaagacgcc gcttatcggg
     3301 cgctgcgcca gggggtgctg gaatacgacg agcgtcagaa ttatcgcggc gcagagggtt
     3361 attttgatct tcccaaggaa gaaatcgggg aagaagcgct tgaggattcg ctgcaggacg
     3421 aactggaaag cgacgacatc tacccggcca tcgcgctgga ggtcgatgcc aaggcagtga
     3481 aggcctaccg caagggcggc gaattcgtca ccatcaccgg cgacgggctg aagttcgcca
     3541 gccgcatgat cggcaatcgc gcgcctgcca aactcaggct gcgccgcggc gcgctgatcc
     3601 gggcgcaacc cgacggcaag ggcggctgga aaatcgtgca gttgccgcag gttgaagccg
     3661 cgctggtgtc cgtaaacacc aacgacggcg caatccgcgc catggtcggc ggattcgact
     3721 tctaccgcaa caagtacaac cacgtaaccc aggcgcgccg gcagcccggt tcgagcttca
     3781 agccgttcat ttattcggcg gcgctggaaa agggtttcac gccagccacc atcatcaacg
     3841 acgcgccgct gaccatcgac gccagcgaaa ccggatcggt gaactgggag ccgagaaact
     3901 tcgacggcac cttcgatggc ccgatccgca tgcgcaccgc gctgaccaga tcgaagaacc
     3961 tggtgtccat acggattctg caggcgatca ctccgcagta cgcacaggac tacatctccc
     4021 gcttcggttt cgatccgaag caacatccgc cctatctgac catggcgttg ggtgccggct
     4081 caaccacgcc cctggaaatg gtgatgggct actcggtgtt cgccaacggc ggtttccgtg
     4141 tgacgcctta tttcatcgag cgcatcgagg acgcacaggg cgaaatcctc ggcaaggcca
     4201 atccgatcca ggttggcaag ggcgcggaac gggtaatcga ttcccgcaac gcgttcatca
     4261 tggcaaacat gatgcaggac gtaatccgcg gtggcaccgg ggcgcgcgcc atggcactcg
     4321 gccgcgtgga ccttgccggc aagaccggca cgaccaacga gcagttggac gcgtggtttg
     4381 ccggattcca gcgcaacctt gcgacagtgg tgtggatagg ctacgacatc ccgcgcacgc
     4441 tgggtgatca tgaaaccggc gcggtggcgg ccctgccgat ctggatgtcg tacatgggga
     4501 cggtcctgaa aggccagccg gaagaactct gggtcgcgcc ccaaggcgtg gtcgcagtcg
     4561 cgatcaatcc gcaaacgggc cttcgcgacc ggcacgccgc cactccgatg atcgagtact
     4621 tctaccacga gaacgtgccg ccggagcagg agagcagccc ggaagaccag cttggcgact
     4681 ctgccaagcc gccggaggaa gtcagggacc agctatactg aacacagatg tcgagggaga
     4741 acggcaataa ccgacagatg cgcgagcgca tcgcccaact tgcggcgcgc ctgatggccg
     4801 tggacgggat cgatgatttt gcgctcgcca agcgcaaggc ggcacgtcag gccggggcgc
     4861 cggacacccg caacctgccc aacaatgagg aggtggagca ggccctgcgc gcctaccagc
     4921 agctctacca ggccgacgag cagcaggcgc gcctgcgccg cctgcggcag aacgcccgcg
     4981 agatgatgca actgctcgcg cagttcgatc cccatttgtc cggttcggtg ctgtccggca
     5041 gcgcgggcaa gtactccgac atcaacatcc accttttcac cgacagcgtg aaggacgtgg
     5101 aaatgtttct gctcaaccgc cagatcccct atcgcagccg cgaacggcgc gtttacattg
     5161 gcggggaaca gcgcagcgta ccgaatttca ccgtcagtac cgacgacgcc gatttcgaca
     5221 tcaccgtgtt cgcgccgcgc gatctgcgct atcagctgcg caccaccgcc gagggcaagc
     5281 cctttgaccg tgcccgcatc gactggctga actcggtgct cgaaagcgct cgaactgcgg
     5341 gcgctgcgac taatcttctt tgagccaggc ggccgcttcc agggcgaaat aagtcaggat
     5401 gccgtcggcg ccggcacgct tgaaggccag caacgactcc agaacgcatt tgcgacggtc
     5461 gatccagccg ttcacccccg cggcctggag catcgcgtac tcgccgctga cctgatacac
     5521 gaaggtcggc gcgccgaact cgtctttcac cctgcgcacg atgtcgaggt aaggcattcc
     5581 aggcttgacc atcaccatgt ccgcaccctc ttctaggtcc attcccactt cccaaagggc
     5641 ttcgtcgctg tttgccgggt cgatctggta agtgtatttg tcgcccctgc cgagatttgc
     5701 cgccgagccg accgcgtcgc gaaatgggcc atagaaactc gacgcatatt tggccgcgta
     5761 agccagtatg cgcgtgtaaa tatgcccact ctcatccagc gcccgacgca gcgccctgac
     5821 gcgcccgtcc atcatgtcgg agggcgcgac gatatccacc ccggcttctg catgcgtcag
     5881 cgcctgtttg accaacgcct cgatggtctc gtcattgagc acgtaaccgc tgtcgtcgat
     5941 caaaccgtcc tggccgtggc tggtataggg atcgagggcg atatcggtga tgacgcccaa
     6001 ttgcgggaag cggcttttca gcgcccggat ggatcgcgga gcgagcccgt cgggattgaa
     6061 cgcctctctg gcatcggcgg tcctgagctt gttgtccacg accgggaaca acgcgatcgc
     6121 cggcacccca agctcgacgc agcgctcggc ctgccggagt aacgcgtcga ccgtcagccg
     6181 ttccacccct ggcatcgacg gaaccggctc gctgcggtgc tgtccgtcaa ttacgaacac
     6241 cgggtagatg aaatcgtccg aagacagtcg atgctctcgc accatgcgtc gggaaaaatc
     6301 gtcgcggcgc agcctgcgca tcctgcggac tggaaacttt ccgagtacat gcatgatcgg
     6361 ccctcgattc cccgcccctt ccggtctccg gttgcaaagc gagaaactat ctcgttgaat
     6421 ttaattataa atcgtcggtt gtgcaaagcg gaactttttt gcgactccag gactctcaat
     6481 caatagacag tgatgtctcc cgcttctcct ccctgagcgg gtgccttaat ccaattgagg
     6541 ctttgtttcg cccccggttt tagtcccctt taaccggggg ttttttattt tagctgtctc
     6601 tgcccaccct acccggcatc gaaccagtcg gccaatagcg tctcggcttc gtcgacgccc
     6661 tgtttggtcg tcgcagaaaa aagttgaacg ctgctgccgt ggtattcggc ggcgagcctt
     6721 tcgcccacta tgcgcaattg tctgctcgct tcctgtttcg acaatttgtc tgccttggtg
     6781 agcagcacgt gcaccggccg gcctgtcggg gcgaaccagt ccagtagctg ccggtccagc
     6841 ggcgtcagcg gatgacgggc atccattacc accacgagcc cgcgcaactg gccacgagcc
     6901 tgcaagtaac cggacagcag ttcctgccag gatcgtttcg tcgctcccgg cacccgcgcg
     6961 tacccgtacc cgggcaagtc caccagaaaa cgcccgaccc ccagactaaa gaaattgatc
     7021 tgctgcgttc ggcccggagt cttgctgacg aaggccaggc ggtgacgccg ggcaagcgcg
     7081 ttgagtgcgc ttgatttgcc cgcgttcgag cgtccggcaa atgccacttc gactccagac
     7141 gaagccggca ggtcgcgctc gtgcgccgct gtagcgaaga aggcgacatc gttgaacaaa
     7201 cgggtgcggc tcatcactta cgctaaaatg ccacctctcg cggggaccag cgcacgcccg
     7261 ggactcggca caaaatccaa ggagtattca tgaatcgtct catcgcggcg gccgtggtcg
     7321 cgctcatatc cgtcacctcg gccgtcgcag ctgattacgg cgacccagcc aaggctcaat
     7381 ccatcgccaa ccaaatctgc gccgcctgcc acggggccga cggcaacagc ccgctgccga
     7441 tgaatccgag tctcgccgga cagcatcccg agtatctgtt caagcagctg aacgaattca
     7501 aatcgggcaa ccgtaacaac gccgtgatga tgggcatggt agccggtctg tctgccgagg
     7561 atatgcgcaa tctttccgct tattacgccg cgcagaaact ccgcgaagcc gcggcgaaag
     7621 acaaggacct ggttgctcat gggcgcaagc tgtttcgcgg cggcaacctc gccaccggcg
     7681 tggcagcctg cgccggctgc cattcgccga acggtgccgg aattccttcc cagtatccgc
     7741 ggcttgccgg tcaacatccc gagtacgtcg ttgcgcaatt gaaagcattc cgtgccggag
     7801 agcgcgccaa cgacaccaat aacatgatgc gcgccgtcgc ggcccggctc accgacaagg
     7861 aaattgccgc cgtcgccgag tacctctccg gtttgcgctg acctcacgac ggttcacgca
     7921 gccgcgactt tcgcgaatgc tgccgatgcc gcggcgatgg tggtgctgat ttcggcgtcg
     7981 ccgtgagcgg cggagacaaa ccccgcttcg aatgccgacg gcgccaggta cacgccttga
     8041 accagcatgg catggaaaaa acggttaaag gcattcttgt cgcattccat gacttgggca
     8101 taactctccg gcgacgaggc gcggaaatac atgccgaaca tcccgccgac cgattgcgcg
     8161 gaaaacgcca atccggcatc tctaccggcc ttctgcaggc cctgcaccag cgccgcggct
     8221 gcattgccaa gccggtcgaa aaaaccgggc gcctggacaa gcttgagcgt ggccaaaccg
     8281 gcagcgaccg cgaccggatt tcccgacagc gttccggcct ggtacaccgg tccaagcggc
     8341 gcaatgaact gcatgatatc gcgccgtccg ccgaatgcac ccaccggcat gccgccgccg
     8401 atgaccttgc ccagcgtggt caggtcggga gtgatgcggt aaagcccctg agctccctgg
     8461 agcccgaccc ggaatcccgt catcacttca tcgaaaatca acaccgcgcc gtgcttcgtg
     8521 caaaggtccc gaagcgcgcg caggaattcc ggcctgggct tgaccagatt catgttgccc
     8581 gccaccgcct cgacgatcac cgcggcaatt tcatcgcctt gcgcgccgaa tgcgcgttcc
     8641 agcatgccag cgtcgttgta atcgagcacc agcgtgtgcg tcgtgatttc tgcaggcacg
     8701 cctgcggaac tgggctgacc gaaagtgagc gagccggaac cggcttttac cagcaacgaa
     8761 tcggcgtgac cgtggtagca gccctcgaac ttcacgatct tcgcgcgccc cgtgaagcca
     8821 cgcgccaacc ggatcgcgct catggtcgcc tcggtgccgg aattcaccag gcgcacctgc
     8881 tgcatcgacg gcagcagccg ggtgatgagt tccgccattt ccagttcggc ttccgtcggg
     8941 gcgccaaagc tcaagccgcg tgaggcagcc tcccgcaccg cttccaccac ttgcggatgc
     9001 gcgtggccaa gcaccagcgg cccccaggaa ccgacgtagt cgacgtaacg cctgccgtcc
     9061 gcgtcccaca cgcaggcgcc ctccccgcgg ctcacgaaca acggcgtgcc gcccaccgag
     9121 cggaaggcgc gtaccggcga gtttacgccg ccgggaatca ccgtctgcga tttctcgaaa
     9181 agaatctcgt ttctggtttt catgcttgca gtctcaggac tcactgcgtt ccgcgaacag
     9241 cccggcaatg cggcgcgccg cgctctcaac gtcgtgcgca tcaaacactg cagcgaccac
     9301 tgccaccgcg tcggcgccgg cttcgatcag tccggaagca ttgtccggcg tgatgccacc
     9361 gatcgcgacg atcgggcgcg aaaacttcct tgccgcgcgc aacaggtccg gcccagccgt
     9421 taccgcgccg ggcttggtcg gcgacggaaa gaaactgccg aacgcgatgt agtccgcgcc
     9481 ctgcaccacc gcatcgcgcg cacgccacaa ctggttgtag cacgacacgc caatcagttt
     9541 gccgggaccg agcagggcac gcgctgcgcc gacgccgtcg tcgtccttgc ccaggtgcac
     9601 tccgtcggca gccacctcgc gcgctagctc gatactgtcg ttaacgatga aacaagcgcc
     9661 gacctcgcgg cacaaccgcg caatccgaag cgcctgttcg cgtcgtaacc gctcgtcggc
     9721 agtcttgttg cggtactgca ccacatgcgc gccgccgcgc aacgcagcct cgaccttgct
     9781 cgtcaaaagc gcggagtcgg caagcccggg cgtcaccgca tacaagccgc taaccgtctt
     9841 gcgcatcctc cggctcttcc tcgcgcgccc agaacaaccg gtccgggatc aactgcccca
     9901 tgccggtccg atacgcgaac ttgagcgtct gccaggtgta ttcctgtgca tcgcgtacag
     9961 cctcgggcac gccgagtccg ttggcgatcg tcgccgcgac cgccgaggcg agcgtgcagc
    10021 ccgagccatg gaaaatgccg ggcaggcgtt gccaggagtc ggttcgtacc acgccctgct
    10081 ggtcgtggag tgtgttgacc acctgcgccg tgttttcgtg ggtcccggtc accagaacaa
    10141 actcgcagcc gagattgagc aggcgccgcg cgcattcgtt gagatcgggg tcgtcgttct
    10201 cgttgctgtc gtcttgcgcc agtcgccggg cctcgaggct gttgggcgtg atcactgtgg
    10261 tctgcgggat cagcaactcg cgcatggccg agatcatgtc ctcggaagcc agttcgtcac
    10321 cgcggcccga ggcgagtacg ggatcgagca ccagcgggat atcgggataa tccgaaacta
    10381 cttcggcaat cgccgctatc gcttcgacac ttccgagcag gccgactttg aacaccgcga
    10441 ccggcacgtc ctcgagcacg cagcgcgcct ggtcggcaac ccactcggca tcgatcggaa
    10501 gcacgtcttc cacgcccatc gtgtcctgga tggtcactgc ggtgatcacc gacaacggat
    10561 ggcaacccat gctcgacagg gtgaggatgt ccgcctgaac tcccgctccg ccgctgggat
    10621 cagtcgccgc gaatacgagc acgatcggaa gagaatctgg cacggcgatg tttcgcggca
    10681 gcgtttcgac ttaaaatgga attttaactg aaaagctccg tcattcccca aaccaacaat
    10741 ggctgcaggc gaaaaggaag aatacaagac ctacatgtgc ctgatctgcg gattcatcta
    10801 cgacgaggca accggcctgc ccgaagaggg cctttctgcc ggcacacgct gggaggatgt
    10861 gccggtcaac tgggtttgcc cggaatgcgg ggcgcgcaag gaagacttcg aactggtgga
    10921 gatatagaat gttgagtgtt aaatgtttag ttttgagtta tcccagatgc cccggcgact
    10981 gaccggaaac ttaacattac attcaaaact caacacttaa cattgcgcgt atgcgtattc
    11041 tgcacacgat gctgagggtg ggcgacctga agcactcgat cgatttctat accaaggtgc
    11101 tcggcatgaa ggtgctgcgc cagaaggact atccggacgg ccgtttcacg ctggctttca
    11161 tcggctacgg cgacgaagac aaggaagcgg tgctcgaact cacccacaat tgggacactg
    11221 cgaaatacga cctgggcaac ggctacggcc atgtcgcgct cgaggtggac gatgcctaca
    11281 aggcctgcga agaagtgaaa aagcgcggcg gcaaagtcac acgcgacgcc ggcccgatga
    11341 agcatggcac cacagtgatt gccttcgtcg aggatccgga cggctacaag atcgaattca
    11401 tccagcgcaa gcgctagctc cgtcgcgctt ccagatagtt cgcgatgagc acgaatgccg
    11461 ggacgcccag cgtctcgcct ctggcgcctg gttcgattcc caactgttcc agttccccgg
    11521 cttcgagcaa ctcccgcaac gcgttgcgca gcgtcttgcg gcgctggccg aacgcgcgcg
    11581 tcaccacatc ggcgaacaag gactcattgc tggcacggtg aggcgggctc gcgtagggag
    11641 tcatccgcgc gaccgaggac cagactttgg gttgcggatg aaatgccgtc ggcggtacat
    11701 cgaacagcga ctcgatgacc caccggtact gcaacatcac tgacaggcgg ccgtactctg
    11761 gcgaaccggg ttcggcggcc atcctctcga cgacctcgcg ctggagcatg aaatggcagt
    11821 cgcgcagcaa ggccacctgc gcgttcaggt gaaacaggat cggagtcgaa atgttgtagg
    11881 ggaggttgcc gactacgcga atgcctgcgc caaacgcgga aaaatcgaat tccagcacat
    11941 ctccctgatg gacttgcagg ctctccggcg ggaaacgcgc gcgcagagcg cggacgatat
    12001 cgcggtcgat ttcgataacg tccagacggg caacgcggtc gagcagcggc gcagtgagtg
    12061 cgcctgggcc cgggccgatc tcgatgatgc ggtcttcgcg ccggggatca atggcggcga
    12121 cgatctgccc gacgatgccg cgatcctcca ggaagttctg gctgaacctc tttctgggag
    12181 tatgcgttgc ctgcaagggg ctggtccgga ccaggcacga ggtccttcga ttacgctcag
    12241 cgctcttcga ggcgcagttc aacgtaggcg cggtcgcgct gctgccgtac ccactcctga
    12301 taggcttcgt cggatttgcg cgcacggatt gcctggcggg ccagcagccg ttgacgctct
    12361 ttggacatgt cctcgttgcg ccgctcgagc acctggatca gatgccagcc gaacgacgac
    12421 tgtaccggct cgctcacctg tcccggttca agtgcgttca tcgcgcgctc gaactccggc
    12481 accgtgtctc ccggcgacag ccagccgaga tcgccgccgc gcgaggaact cgcgtcctcg
    12541 gactgcagcc gcgccagttc ggcgaaatcg gcgccgttgt cgatgcgctc cttgagatgg
    12601 aacaggcgct gtttcgcgtc ggcctcggaa acgacttcgt tcagacgcac caggatgtgg
    12661 cgtgtgcggg tctgctggac aatcaccggc gtttcgttgc cgcgcttgtc gaggagcttc
    12721 aaaatgtgga agccgttggc gctgcgcagc actgcgctca agtcgccgac cttcatggtt
    12781 ctcaccacgt cgacgaagat ctgcggcagg cgcgaagccg ggcgccaacc cagcgggccg
    12841 ccttccagcg cattctgcgc gtcggacact cccgccgaca cctggcgaaa atcagcaccg
    12901 cccctgagtt gcgccagcgc cttctccgcc gcagagcgct tggcctggat ttgctccggg
    12961 ctggcctgct cggggaccag caccaggatg tgcgcgaggc tgtactcgtc atcctttccg
    13021 cccaggttct gctgggtctt gagatagttg tcgatctctg catctgtgat cagtatcttg
    13081 ctgtcgactt cgcgctcctt gaggcgcgtg atcaggatct cgttgcgcag ctcgtcgcgg
    13141 aagcggttga aattgacgcc gtcgctttcc aacgccttgc gaaaggcgtc gggcgtcagt
    13201 ttgttctcct gggcgatgcg ttccagcgag cgctgcagct gggcatcgtc cacccgcaaa
    13261 ccggtctcct tggcagtctg cgccagcacc cgggcggtaa ccagccgctc cagcaactgg
    13321 cgctccagca cgtcctgagc gggcagtggg gtgttctggc gccgcagctg ctgcagcgcc
    13381 accctcactt gctcatcgag atcgagccgg gtgatgactt cgttgttgac cacggccacc
    13441 acgcgatcca gaagtgcggg gtcgccgtgc gtacgctcgc cctgagccgc gccggccggc
    13501 gcgataccaa gcaatgcaag aaacaaagcc agtattcgca ttttcgatgt catggcaaga
    13561 cgatgccggc actgatgccg ccagcagggg cgtcagcagc tgacattacc gctttcaata
    13621 cgccgggaac ggattgcgtt ctgccggcgg cggttcgttg gtcttggtgt aaccggtgac
    13681 gttctgccgc agcagctcga gcgggttgga gccgatcttc gacacgccgt tcaactcgac
    13741 ctgaaagaac atcgcgttga cgtattcctg cgtcgcgctg acgaaccgat gcaccacaaa
    13801 acgggccgcc cagcagccag cattgtactc gacccccgcc agaccctcca gcagacgctt
    13861 gtcgcgcaag gtgtagttcc agcgcgccaa cccggtccag cgcccggcga tcggccactg
    13921 cgacgacaga tccacctgct ccagggtatc gcgcgtaaag cgatagccga aattcatcat
    13981 cttgccgatt gctggctggt agcgcagcgc cacattgctg cgttccgttc ggctggtaat
    14041 ggcgctgtac tgcaggccga catcggccac ccagttgggg gtgatcctgc cggtcatcgc
    14101 ggccaggata tcggaacgat taaaatcccg ccgcgacgtg ctgagggaaa cctgctgctc
    14161 tttgaaatag tagcgctggc cgagaacgaa gcgaagttgc tcctctccgc tgccgggatt
    14221 gatcagccgg ctcgacagcg ccgccgtgag ctgattggcg tcgttgatgc ggtcgccgcc
    14281 ggtaaactgg ttttccgtga agatctgcgc caggttgaag tctgccacgg ccgtgtcgaa
    14341 gactggcaac tggtcctgcg gacgaaacgg aatgtagaca tagtaaaggc gcggttccag
    14401 cgtctggatg aaatcgcggc cccagagccg ggcattgcgc tcgagagtta ctgcgctgtc
    14461 gaggctgtag atcggcaatc cccgcgtttc atcggggaga ttcggatcct cgaaggaata
    14521 gcgggtattg tgatagccga gcttgggcgt caggtagaaa tacgaggtct gcagcggcac
    14581 gctgatcgaa ggatagagaa tctggcgtct gccgttcagc aggaacggat gatcgaagtc
    14641 cacgtactcg ccctgcaggc cgagatctag gtaatagtca gtctggcgat tgacgttcag
    14701 ggtaagctgc ggcgcgcgcg catacggcgg tgtgaccggg gccagcggat cctgcagggt
    14761 ctgaaagcgt tgcacgcggg cgttcaggtt ccaccagtcg ccgtcgtaca acaaattgcc
    14821 ttcgcgcggc aggttggtct gcgaagtcac cgcgatcttg tcgctcaggt cggtgaaaaa
    14881 ggtgtcgtct gatacgccct gcaggttgaa tccccccgag ataccacgac cgaatagctc
    14941 ctggtgttgc caggagtagc catagcgact ttcctgtttc tgccggtcgt tcggaaggta
    15001 ttccgtgcgc acttcaccgc tcagactcgg ctcgagatag cgcagttccg tgttgagcag
    15061 cacaccgcgc ttggcaagga tgcgcggcgc gatggtggcg tcgcgattgg gtgcgatgtt
    15121 ccagtagaac ggctgcgtaa cctcgaagcc gctttgcccg gtgctgccga tggtcggcgg
    15181 cagcaaaccg gtcttgcgac ggccgctcag cgaaaagtcg agataaggtg aatacagcac
    15241 cggcaagccc ttgaatacca cagtagcgtt atgcgccacg cccacatcgc gttgccggtc
    15301 cagatccagc ttgtccaccc gtaggaacca gtcgtcgtcg ccgacgtcgc agttggtgta
    15361 ggtagccttg tccgcccgta tcttggtgcg gctttccatc accatccgat cggcactgcc
    15421 gcgcgcccgg aactgctgga aattgtatgc cggcttctca atgtaaccgc tttcgctgtc
    15481 gaggttgtaa aacaacttct cgccggtgac cacgtcgcct tctttttcga agcggacatg
    15541 gcccgtgacc gtcagttccc gctccggaaa ggcgtaatac agatagtccg cgaaaaccgc
    15601 tgctccgcgc ttgcgcaggc ttacgttgcc ttcggcctcg agctcgcgct cctgatggcc
    15661 ctgcagccgg tcggcttcga gaaacatcgg cgtgggctgg ttgctgccct cggaatacgg
    15721 aatcaacgcc ggctgaattt tcagcttgag accaggttcg tcggcgccgg cgccgggggc
    15781 aaggaagcag atcaacaaga cgatcggcga ggcgaagcgt ggtcgatgca tgcgggctgt
    15841 tgatcgaggc gaggtgctaa aatctcacaa aatcacagtc aaggcaacga agccccttgg
    15901 accgctacgc ttctctacag gattggatcg cgcgccagtt tcccggaata acgttcaccc
    15961 tctcgccggc ttctgtcgac gccagcttcc gccgctactt tcgcgtcact ttccccgatt
    16021 ccacgctgat agcgatggat gcgccgccag cgcaggaaga ctgccgtcct ttcgttcgag
    16081 tggccgcact gatggccgaa gcgaaagttc acgtgcccaa ggtgttggcg cacgaccttg
    16141 cgcgcggctt tctgctgcta tcggatctgg gcgccaccac ttacctcgcg gcgctcaatc
    16201 ccggcaacgc ggaccagctt ttcggcgacg cgatcgacgc gttgatccgc tggcagcttg
    16261 ccagccgcga aggcgtactg cccgcctacg acaagacctc gctgaagcgg gaactcgatc
    16321 tttttcccga gtggtacctc gaccgccacc tcggcatcaa gctaagctcc ctgcaacgcg
    16381 agtcgctcga aaagatcttc agcctgatcc tcgccagcaa tctggcgcag ccgaaagtct
    16441 atgtccatcg cgactttatg ccgcgcaatc tgatggtgag caacccgaat cccggcgtcc
    16501 tcgactttca ggacgccgta tttggaccga tcggctacga tgtggcgtcg ctgttcaagg
    16561 acgcgttcct gagctgggaa gaggaaaggg tgctggactg gacggtgcgc tactgggaaa
    16621 aggcgaagcg tgcgggattg ccggtggatg ccgatttcgg cgtattctac cgcgacgtcg
    16681 aatggatggg gctgcaaaga catatcaagg tgctcggcat ctttgcgcgc atctgccacc
    16741 gcgacaacaa gtccggctac gtcgaggacg caccgcgctt cctggactac gtacgcgttg
    16801 tggcgcggcg ttatgctgcg ctcgcgccgt tgttgcggtt gctcgatcaa ttccaagacc
    16861 gcgagccggc cgtcggtcac accttctgag cgtggccgtg ccttccgctc cgtccgcgat
    16921 gatccttgcc gccggccgcg gcaaccgcat gcgccctctg accgatctca cgcccaaacc
    16981 cctgctcgcg gtgggcggca agccgctgtt gttccggcat ctggagaaac tcgcgcacgc
    17041 cggattcgga acggtcacga tcaaccacgc ccatctcggc gcgcagatcg aagcggcggt
    17101 gggtgatgga ggccgctgga acctgcaggt gcgctactct gccgaagccg aatcgctgga
    17161 aacagccggc ggcatccgca atgcgttacc gctgatcggc ggtcgcactt ttgccgtgat
    17221 aaacggcgac gtgtattgcg actacgacta tgcgggcctc gccgccgcga tcgagcggct
    17281 cgcggattcg caggccctcg cgcatctggt gctggtggac aatccgccgc accaccctaa
    17341 cggagatttc tgtctcgagg ccggactggt gcgctcctcc ggctggcaac cgctgacatt
    17401 cagcggcctg ggcgcgtatc gcgccgaact gttcgagtcg cttgcacgcg gagccaagca
    17461 cgcgctggcg ccgctgctgc gcagtcagct gccccaggac aatgtcaccg gcgagcacta
    17521 ccgcggcctg tggaccgacg tcggcactcc cgggcgcctg gcggaactca acgcgcgact
    17581 gcaaccggga gcacggacgt gattcacatt cccgaacatt tcgccgagca cgacatcgcc
    17641 cgccttcacg cgctggtccg tgcccacaat ttcgccacgc tggtgtcggc gcaccagggc
    17701 gagatccagg tttcccatct gcctttcctg ctggacgcgg ggggcaaact gctgcgcgcc
    17761 cacatggcgc gcgccaaccg gcagtggcgg acattctcgc ccgagcgcga ggtagtggtg
    17821 atcttccacg gcccgcatca ctacgtctct ccggcctggt acgcatccca cccgagcgtg
    17881 ccgacctgga actacgcggt ggtgcacgcg cagggccggc caaggctgat cgaagaccgc
    17941 gagcggctgg cggcgctgct ccgggaactg gttacgcgcc acgaagccgc ctcacccgaa
    18001 ccctggaaaa tggacttgcc tccggactac caggacaaga tgctgtcggc catcgtcggt
    18061 ttcgagatcg agatcacgcg actggacggc aagttcaagc tgagccagaa ccgcccggag
    18121 agcgatcggc cgctggtgat cgaggcgctg gagcggctcg gcagcgacga cgcactcggt
    18181 gtcgccgcgc tgatgcgcga gaatcaaggg aagctctgag ccggaacgga ctgccgcggc
    18241 gcaggccgta taatctccgt tcgtttcccg tacttcccga agatggacaa ccacagactg
    18301 caagcgcaac gccgcgaacg gctggtctcg ctgatcaagc aaggcgttgc cgtggttcgc
    18361 accgcgcccg agcgggcgcg caaccgcgat tccacctacc cgtatcgctt cgacagctac
    18421 ttctactatc tgtcgggttt cccggagccg gaagcggtgc tggtcctgat cgccggcgcc
    18481 aagccaaaga gcatcctgtt ctgccgcgag aagaatccgg agcgggaaat ctgggatggc
    18541 ttccgttacg gtccggacca ggcgcgcgag atcttcgcat tcgacgaggc gcatccgata
    18601 tccgatctcg acgagctgat gccgcagttg ctcgccgacc agccgtcggt attctgcgat
    18661 gtcgggtcgg atggcgactg ggacagccgc gtgatcggct ggatcaacaa ggtacgggcg
    18721 caggttcgca gcggcgtcac cgcgcccgcg gagatctgcg acattcgtct attcgtcgac
    18781 gacatgcgcc tgatcaagga cccgcacgaa ctcggcacca tgcgccgcgc cgccgccatc
    18841 tccaccgaag cacatcgccg cgcgatgcgc gcgaccaggc ccgggctgcg cgagtacgag
    18901 gtcgaagccg aaatcgttca cgagttccgc cgccgcggag cacaagcccc cgcctatact
    18961 ccgatcgtcg ccggcggcgc caactcctgc gtgctgcact acgtgcagaa cgacgccatt
    19021 ctcaaggatg gcgatctgct cctgatcgat gccggctgcg aactcgatgg ttacgcctcg
    19081 gacatcaccc gtactttccc ggtcggcggc aagttcgcgg ggccgcagcg cgacatctac
    19141 gaaatggtgc tcgcggcgca ggctgcggcc atcgccgagg tcaagccagg ggccgaatgg
    19201 gaaaacccgc acaacgcggc ggtgaaagtg ctggcgcagg gaatggtgga cctcgggctg
    19261 tgcaaaggta ccgtgaacag tgcgatcgaa tccggagact accggcgttt cttcatgcac
    19321 cgcaccggcc actggctggg cctggacgtg cacgatgccg gcgattacaa gcgcaacggc
    19381 aagtgggtgg aactcaatcc gggaatggtg ctgacggtcg agcccggctg ctatgtgcgg
    19441 ccgggcgagg gcgtgccgga gaagttctgg aacatcggcg tgcgcatcga ggacgatgcg
    19501 ctggtcaccg gcaacggttg cgagatcatc accacggcgg cgccgaagaa gatcgaagac
    19561 atcgagtcgc tgatgcgcgc ctgacaccgg ccctcttcgg caccatgagc gaaagcgaac
    19621 acgacctcgt catcgtcggc ggcggtccgg tcggggccgc attggcaatt gcgctttccg
    19681 acgccagcca cgacgtgctg ctgctggaag cgcggccgga gctggacacg gcaagcgacg
    19741 gtcgcacgct cgccatgtcc tacggcagcc gcctaatact cgcgcggctc ggcgcctgga
    19801 agcgcgtcga gccggcgaca tcgatccata gcattcacat ctcgcagcga ggcgggttcg
    19861 gccgcgcgct gctggccgcc gaagatgccg gtctgccggc gctcggttac gtcgtcgccc
    19921 atgcgcggct gcagaagatg cttggcgagg cgctcgcctc gagccgggcg acgctggcac
    19981 tgggcgccag cgttgctgac atcgagttca gcgccgatcg tgtcgccatt cgtttccgtc
    20041 gcggcgatca ggagcactgc gtaggtgcgc gcctgctggt cattgccgac ggcggcgcca
    20101 gcctgggaaa acgcgccggt gcaactatca aggttcgcga ctaccggcaa gacgccatcg
    20161 tcgcaactgt cgcaacctcg agaccgcacc gccaacgcgc attcgagcgc ttcaccgctg
    20221 acggtccggt ggccttgctg ccgttcgaag accgctacgc gctggtctgg actgcggcac
    20281 ctgaagccgc ggcccgattc accgagatga acgacaagag cttcctcgag cgcctgcagc
    20341 tgcacttcgg cgaccgcgcc gggcgcttcc tgtccgtcac ggcgcgcgcc cggtttccgc
    20401 tcgcgctcag gtatgccgcc aatcccgtcc ttccgcgcag cgtgctgctt ggcaatgccg
    20461 cccaggcgct gcacccgatc gcgggccagg gattcaatct gggcttgcgc gatgcgtggg
    20521 aactcgccga gctgctggcg cgccgtgatg atcgcgatcc gggaagtgcg gcctgccttt
    20581 ccgctttccg ctcggctcgc agactggaca ggctgggagg aatcgccatt accgactcgc
    20641 tggtcagaat cttttccaac gatttcgtgc ctttgcgcgc tgcacgcggc gccgggctgg
    20701 cgctgctcga catcgcgccg ccggtcaagc gcgcattcat gcaaccgatg atcttcggcg
    20761 cgagcttgta accaaggttg taacaacggg tagcaattgc gcgccctcaa caccgcatcc
    20821 tttacgctaa aatggcgctc ctctttcggt gcccgcataa atgcacatcg gtccgcacgt
    20881 tctcaaaaac aatctgatcg tcgc
//
LOCUS       DASQOP010000130        11594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2472275, whole genome shotgun sequence.
ACCESSION   DASQOP010000130 DASQOP010000000
VERSION     DASQOP010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11594
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2472275"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VE030_05310"
     CDS             complement(<1..767)
                     /locus_tag="VE030_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HZF22870.1"
                     /translation="MAITEEQVRTALQELTDPVTGKDYVSGKEARNIKVEGNDVSLDI
                     LLGYPAKSVIDVVRRDVIAKLKTIAGAGNVSANVQVKIIAHAVRPGVKLMPGVKNVIA
                     VASGKGGVGKSTTAVNLALALAAEGAQVGLLDADIYGPSQQMMMGLSGRPESADGKTI
                     EPKLKYGVQAMSIGLLIDVDTPMVWRGPMVTQALEQLLRDTQWKDLDYMVVDLPPGTG
                     DIQLTLSQKVPVTGAVIVTTPQDIALIDARKGLKMFE"
     gene            878..2965
                     /gene="metG"
                     /locus_tag="VE030_05315"
     CDS             878..2965
                     /gene="metG"
                     /locus_tag="VE030_05315"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HZF22871.1"
                     /translation="MSRRILATSALPYANGSIHLGHLVEYIQTDIWVRFQKMQGHEVH
                     YVCADDTHGTPIMLRADKEGITPEQLIARVYGEHTRDFAGFHVAFDNYYTTNSPENQA
                     FCEDIYRQLDKRDLIARRSVEQFYDPVKQMFLPDRYIKGECPNCHSKDQYGDSCEVCG
                     KTYTPTDLIDPYSVVSGAKPQRKASEHHFFKLSDERCEKFLREFTQTSDALQPEAANK
                     MKEWLGEPGDSKLADWDISRDAPYFGFPIPGTNNQKFFYVWLDAPVGYFGSFGNYVAK
                     QKAASKPIDQDAFLRPGGDTEMVHFIGKDILYFHALFWPAMLKFSGYRVPTKVFAHGF
                     LTVDGQKMSKSRGTFITAESYLKQGLNPEWLRYYYAAKLNSTMEDIDLNLEDFVARVN
                     SDLIGKYVNIASRAASFITRFFNGELAYHSDTKSLVEGARASAFLSKEAYDAREFGKA
                     IRQAMEIADRINEWFDKTQPWNLAKEPSKAKELQDVCSRCLHGFQLLTVILAPVLPKV
                     AERVARDLFGMEREFNWDDASALPTRINPYQHLMTRVDPKQITALVEANKESLQPALQ
                     SHSPQRHAQHQEKAVETTTAPSFISIDDFNRVELRVGRIVSAEHVEGADKLLKLQLDL
                     GNETRQVFAGIKAAYDPAKLVGRLTVLVANLQPRKMRFGESQGMVLAASGDGPGIFLL
                     SPDDGAQPGMRVK"
     gene            2975..3256
                     /locus_tag="VE030_05320"
     CDS             2975..3256
                     /locus_tag="VE030_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HZF22872.1"
                     /translation="MKITRRLRIRGQVQGVNLREAMLLRAEQLKVTGWVQNRLDGSVE
                     AIVQGEAFAIDAIVDWAHQGPPGARVDSLDVETADAEGDYDSFDKKPTA"
     gene            complement(3306..7643)
                     /locus_tag="VE030_05325"
     CDS             complement(3306..7643)
                     /locus_tag="VE030_05325"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZF22873.1"
                     /translation="MSALENFHPAVARWFGRAFAAPTEPQTQAWPAIQSGNHVLIAAP
                     TGSGKTLAAFLAAIDALVRLGVRGELRDETHVVYVSPLKALSNDIRKNLEEPLAGIRQ
                     ELAAAGLPDLEIRTLVRTGDTPRADRERMRRKPPHILVTTPESLYILLTSDSGRRMLS
                     TARIAIVDEIHALAPNKRGVHLALSLERLQHLTGGRLTRVGLSATQKPIEDVARFLVG
                     GGHSGQLLGPGACTIIDSGHVRRRDLAIEVPESPLEAVMSAEVWTQVYDRLAELIREH
                     RSTLVFVNTRRLAERVARALSERLGEDQVTSHHGSLAREQRLNAEQRLKRGELKALVA
                     TASLELGIDIGHVDLVCQISTPRSISALLQRVGRSGHAVGGLPKGRLFPLSRDDLVEC
                     AALLDGVRRGELDRLRIPRQPLDVLAQQIAAEVAGGEWDEDALYELVRRAWPYRDLTR
                     EEYSEIVRMLAEGFSMRRGRNRALLHRDAVNRKLRPRPGLKLTAVTSGGTIPDTADYT
                     VVLSPQDVVIGTVNEDFAVESLAGDVFQLGNTSYRILRIEAGRVRVEDAAGMPPSIPF
                     WLGEAPARSDEVSAAVSRLRADLEARLDDRAAALSYLVEEVGVAAEASEQIVDYFTTA
                     RAVLGTLPTQQRLVLERFFDESGGTQLVIHSPYGNRINRAWGLALRKRFCRKFNFELQ
                     AAATEDAIILSLTSTHSFELEEVIRYLHSNNARQVLVQALLDAPMFMTRWRWDASIAL
                     ALPRFRGGTKVPPQLQRMQAEDLVAAVFPDQLACQENVVGDREIPDHPLVRQTIDDCL
                     HEAMDIEGFERLLRALERGELEVVARDLVEPSPFALEILSARPYAYLDDAPLEERRTQ
                     AVVSRRWLDPESAADIGRLDPEAIARVKEEAWPQADTADELHDALAWLGLLTEAEVDA
                     QPAWRELLNRLGDDKRVTRFLPSERPDSAGFFVIAERLPEFSAVHPGAVLKPSVEAPA
                     GYAKTWSRDEALVEIVRGRLEGLGPATAAELAGPIAVPVADIDRALLALEAEGFVMRG
                     SFTAIGIAQWCERRLLARITRYTVKRLRQEIEPVSAADFMRFLLDWQRVSERMEGPDA
                     VAAVVSQLEGFEAAAASWETELLPARVAGYEPDWLDDLCRAGRVVWTRLDAPRLDPQR
                     GHGPSPVRSTPITLMTRKNLPLWMALANGSNETVHLSTRAQAVADYLAQQGASFFDDI
                     AGGLDLPRTFVEDALRELTAAGLVNSDGFIGLRALLLPADRRKPISGSRRRRTAAFGV
                     EDAGRWALIRRKAAGAEISSTLARETAEQIARTLLKRYGVVFWRLIAREADWLPPWRE
                     LLMAYRRLETRGEIRGGRFVAGFSGEQFALPEAVGVLRGVRNQEKTGLPVAVSGVDPL
                     NLAGVLLPGSRVPAVYSNRILYRDGVAIAALVAGEVQYFENLEPEKAWELRNVLLRVP
                     APASLAALM"
     gene            complement(7677..8168)
                     /locus_tag="VE030_05330"
     CDS             complement(7677..8168)
                     /locus_tag="VE030_05330"
                     /inference="COORDINATES: protein motif:HMM:TIGR04366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HZF22874.1"
                     /translation="MNEPTVIGPALLDQIWGQARESPRKRKNFNFHLRESDPASRLLN
                     AVEPDSYVRPHRHMDPAKDETIIAVRGSFGVVFFDERGTVTKSLIIRAGGETMGVNIP
                     HGIYHTLVALDSASVFFEAKAGPYVPVSMAEVASWAPAEGDPGALAYLEKLRGLFDFQ
                     LAD"
     gene            complement(8165..11593)
                     /gene="dnaE"
                     /locus_tag="VE030_05335"
     CDS             complement(8165..11593)
                     /gene="dnaE"
                     /locus_tag="VE030_05335"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HZF22875.1"
                     /translation="MQPKFIHLRLHSEYSIVDGIARLDGAVATAKADSMPALALTDLS
                     NVFGMVKFYQAARSQGLKPIMGCDVWISNDADRDKPFRVLLLCASSAGYLRLCDWLTR
                     AYRNNQHRGRAEIHKKWLSETGTEGLIVLSGAHLGDIGHALLADNPQQAQKLAQDWAQ
                     LFPKRFYIELQRAGHAHAEHYVKRAVRLASGLKLPVVATHPVQFLKREDFVAHEARVC
                     IAEGYVLSDQRRPRHFTEEQYFKTQAEMTELFADLPQALDNSVEIARRCNFPLDLGKS
                     RLPLFPTPEGVSLEQYLHERSVAGLELRMEALYPEARAEHVPRYRERLEFEIKTIVHM
                     GFPGYFLIVADFINWAKANGIPVGPGRGSGAGSLVAYSLGITDLDPLRYDLLFERFLN
                     PERVSMPDFDVDFCQDGRDRVIEYVRHKYGEESVSQIVTFGTMAAKAVVRDVGRVMEW
                     NYSRTDELAKLIPFQPGKLVTLKDAREMEPRLKEREESEEETRELLALAEQLEGLTRN
                     VGMHAGGVLIAPGKLTDFCPLYAPSGSDSAVSQLDKDDVEAIGLVKFDFLGLTTLTIL
                     DWTLRFIKRLDPDSEIRLEQLPLDDPAAFKVFAGANTTAVFQFESRGMRELLKQARPD
                     RFEDIIALVALFRPGPMELIPDFVKRKQGQERFEYLDKRLETILSPTYGIMVYQEQVM
                     QIAQVIGGYSLGAADLLRRAMGKKKPEEMAQHRDIFKAGAMKNGVSEHRAEQLFDLME
                     KFAGYGFNKSHAAAYALVAYQTAYFKAHHAAAFMAANMSAVMDDTDKVQQFCEDTIAN
                     GLEVLPPDIHAAEYRFVPVDAKRIRYGLGAVKGTGESAITHIIEARQQGGSFKDLFDF
                     CRRVDKRVVNRRVIESLVRAGAFDSINDHRASLMASVGIAMEAAEQENRAASQNSLFG
                     DASATAEGPKLIEAARWEAKEQLQNEKLALGYYFSGHPFQSYAAEVEAFARTRLANIQ
                     PQGQTVIVAGVIYSIRQQQTRRGRMAVVVLDDGTARVELTVFNELFESHRNWLKEDQL
                     LIVEGKASFDNFTQGLRLTADRVYDLAAARSRFARKVRLHCNGQSSGSKLKELLAPYR
                     DAGDGGCPVSVVYSNDAAVAEIELGDAWRVKLDDGLINSLGNWIEPKNVQIVYP"
BASE COUNT         2128 a         3780 c         3591 g         2095 t
ORIGIN      
        1 ttctcgaaca tcttgaggcc cttgcgggca tcgatcagcg caatgtcctg cggcgtggtc
       61 acgatcaccg cgccggtcac cggcaccttc tgcgacagcg tgagctggat gtctccggtc
      121 ccgggaggca ggtccactac catatagtcc aggtccttcc actgggtgtc gcggagcagc
      181 tgctccagcg cctgcgtcac catcgggccg cgccagacca tcggcgtgtc cacgtcgatc
      241 agcaatccga tcgacatcgc ctgcacgccg tatttcagct tcggctcgat ggtcttgccg
      301 tcggcggact ccggccggcc ggagagtccc atcatcatct gctgggaggg gccatagata
      361 tcggcgtcga gcaagccgac ctgggcgcct tcggccgcga gcgccagcgc caagtttacc
      421 gcagtggtgg atttgcccac cccgcctttg ccggacgcca cggcgatcac gttcttgacg
      481 ccgggcatca gcttgacgcc gggccgcacc gcgtgggcga tgatcttgac ctggacgttg
      541 gcgctgacat tccccgcgcc ggcgatggtc ttgagcttgg cgatgacatc ccgccgcacc
      601 acgtcgatca cgcttttggc cgggtaaccg agcaggatgt cgagggagac gtcgttgccc
      661 tcgaccttga tgttgcgggc ttccttgccc gagacgtagt ccttcccggt caccgggtcg
      721 gtgagttcct gcaaagccgt cctgacctgc tcttcggtga ttgccacgcc tgctgccctg
      781 gattttaagg ggcgaatttt aagcttaatt cccggcgcgt aaagggcttt ctcggggtcc
      841 atttgataga atccgagctt ttccgcgtct ccacgaaatg tcgcgcagga tcctcgccac
      901 ctcggcgctg ccgtacgcca acggcagcat ccacctcggc cacctagtgg aatacatcca
      961 gaccgacatc tgggtgcgct tccagaagat gcagggccat gaggtgcatt acgtatgcgc
     1021 cgacgacacc cacggtacgc cgatcatgct gcgcgccgac aaggaaggca tcaccccgga
     1081 gcaactgatc gcccgcgtat acggcgagca cacccgcgac tttgccggtt tccacgtggc
     1141 gttcgacaac tactacacca cgaattcccc ggagaaccag gctttctgcg aggacatcta
     1201 ccgccagctg gacaagaggg atctgatcgc acggcgttca gtcgagcagt tctacgaccc
     1261 ggtcaagcag atgttcttgc cggaccgcta tatcaagggc gagtgcccga actgccactc
     1321 caaggaccag tacggcgatt cctgcgaagt ctgcggcaaa acctacaccc cgaccgatct
     1381 catcgaccct tattccgtcg tgtcgggagc caagccgcag cgcaaggcgt cggagcatca
     1441 tttcttcaag ctctccgatg agcgctgcga gaaatttctg cgcgagttca cgcagaccag
     1501 cgatgccctc cagcccgaag cggcaaacaa gatgaaagag tggctgggtg agcccggcga
     1561 tagcaagctg gcagactggg acatttcccg cgacgccccc tacttcggct ttccgatccc
     1621 tggcaccaac aaccagaagt ttttctatgt ctggctggat gcgccggtgg gctacttcgg
     1681 cagtttcggc aactacgttg ccaaacagaa agcagcgagc aagcccattg accaggacgc
     1741 cttcctgcgc ccgggcggcg ataccgagat ggtccacttc atcggcaaag acattctgta
     1801 tttccatgcc ctgttctggc cggcgatgct gaaattctcc ggctaccgcg tgccgaccaa
     1861 agtctttgcc cacggatttc taacggtgga cggacagaaa atgtccaagt cccggggcac
     1921 cttcatcacg gcggagagct acctcaagca agggctgaat cccgagtggc tgcgctacta
     1981 ctacgcggcc aagctcaaca gcacgatgga ggacatcgac ctcaacctcg aagacttcgt
     2041 cgcgcgggtg aacagcgatt tgatcggaaa gtatgtgaac atcgcgagcc gggcggcgag
     2101 tttcatcaca aggtttttca acggggaact tgcatatcat tcggatacca agtctctagt
     2161 tgaaggcgct cgcgcctcag catttctctc taaagaagca tatgacgcac gtgaatttgg
     2221 aaaagcaatt cgtcaggcga tggaaattgc tgaccgaatt aacgaatggt tcgacaaaac
     2281 ccaaccatgg aacctcgcaa aggagccgtc caaggctaag gaattacagg acgtatgctc
     2341 ccgatgctta catggttttc agttgttaac cgtaattctt gcgccagtgc ttcccaaagt
     2401 cgccgagcga gtagcgcgcg atctttttgg catggaacgt gaatttaatt gggatgatgc
     2461 gtcggcgttg cccacacgta tcaatccgta ccagcacctg atgacccgtg tcgatccgaa
     2521 acagatcacc gcgctggtgg aagccaacaa ggaaagtctt caaccggcgc tgcagagcca
     2581 ttcgccgcag cgtcacgccc agcaccagga gaaagccgtg gaaaccacta ccgccccgag
     2641 tttcatcagt atcgatgact tcaacagggt cgagctgcgc gtcggtcgca tcgtcagcgc
     2701 cgagcacgtg gaaggcgccg acaagctgct caaactgcag ctcgatctcg gcaacgaaac
     2761 ccgccaggtg ttcgccggca tcaaggcggc ttacgatccg gcgaagctgg tcgggcggct
     2821 tacggtgttg gtcgccaacc tgcagccgcg caagatgcgc ttcggcgaat cgcagggcat
     2881 ggtactggcc gccagcggcg acgggcctgg cattttcctg ctctcgcccg acgatggcgc
     2941 tcagccgggc atgcgcgtaa agtaaacaaa ccccatgaaa atcacccgac gacttcgcat
     3001 ccgtggccag gtccagggcg tgaacctgcg cgaagccatg ctcctgcgcg ccgagcagtt
     3061 gaaggtcaca gggtgggtgc agaaccgtct ggatggatca gtagaggcga tcgtgcaggg
     3121 ggaggctttc gcaattgacg ctatcgtcga ttgggcacat caaggcccgc ccggtgcgcg
     3181 ggtggatagc ctggatgtcg aaacggccga cgccgaaggc gactacgact ccttcgacaa
     3241 gaaaccgacc gcgtgaaaat caatctctca ccgtgtttag cgcttaattg acgcgccgac
     3301 gcgcgtcaca ttagagcggc aaggcttgcc ggcgcgggaa cgcgcagcag aacgttgcgc
     3361 agctcccatg ccttttccgg ttccagattc tcgaagtact gcacttctcc cgccaccagc
     3421 gcagcgatcg ccacaccatc gcggtacaga atgcggttgc tgtaaactgc cggcacccgc
     3481 gaaccgggta acaaaacccc cgccaggttg agcggatcga ctccgctgac cgcgaccgga
     3541 agaccggtct tctcctgatt gcgcaccccg cgcagcacgc cgaccgcctc cggcagcgcg
     3601 aactgctccc ctgaaaaccc tgccacgaag cgcccgccgc ggatctcgcc gcgcgtctcc
     3661 aatcgacgat acgccatcag cagttcacgc catggcggca gccagtctgc ctcgcgggca
     3721 atcaagcgcc aaaacacaac gccatagcgt tttagcagcg tgcgcgcaat ttgctccgcg
     3781 gtttcgcggg ctagcgtcga agatatttcg gcgcctgcgg ccttgcgccg gatcagcgcc
     3841 cagcgcccgg cgtcctcgac accgaacgcc gcagtacggc gccggcgcga acccgatatc
     3901 ggcttgcgcc gatcggcggg cagcagcagc gcgcgcaagc cgatgaagcc gtctgaattc
     3961 accaggcccg ctgcggtcaa ctcacgcagg gcatcttcga caaaggtgcg cggcagatcg
     4021 aggccgccgg cgatgtcgtc gaagaaggaa gcaccttgct gcgcgagata gtcagccact
     4081 gcttgggcgc gcgtgctcaa gtgcacagtt tcattcgatc cattggcaag cgccatccac
     4141 agcggaagat tcttgcgggt catcagggtg atcggggtgc tccgcaccgg actcggtccg
     4201 tggccgcgct gcggatcgag tcgcggcgcg tcgagccggg tccagaccac ccgcccggcg
     4261 cggcacaaat cgtcgagcca gtccggctcg tagccggcga cgcgcgccgg gagcaattcg
     4321 gtttcccacg aagcggccgc ggcctcgaat ccttcgagtt gcgaaaccac tgcagccacc
     4381 gcatccggac cttccatgcg ttccgagacg cgctgccagt cgagcaggaa gcgcatgaag
     4441 tcggcggcgg agaccggttc gatctcctgc cgcagtcgct tgaccgtgta gcgggtaatg
     4501 cgcgccagca atctgcgctc gcaccattgc gcgatgccga ttgccgtgaa actgccgcgc
     4561 atgacgaagc cctccgcttc gagcgcgagc agggcgcgat cgatgtcggc caccggcacc
     4621 gcgatcggtc cggccagttc ggccgctgta gccggtccca acccctccag ccgtccgcgc
     4681 acgatttcga cgagtgcttc atcgcgcgac caggtcttcg cgtacccggc gggggcttcg
     4741 acagaaggtt tcaaaacggc gccgggatgc acggcactga actcaggcag ccgctccgct
     4801 attacgaaga aaccagccga gtccggccgc tccgagggca agaaacgcgt gacccgcttg
     4861 tcgtcgccga gtcgattcag cagttcgcgc cacgccggct gcgcatcgac ttccgcctcg
     4921 gtcagcaagc cgagccaggc cagggcgtcg tgcagttcgt ccgcggtgtc ggcctgcggc
     4981 caggcttcct ccttcacgcg ggcgatcgcc tcgggatcga gcctgccgat gtccgctgcg
     5041 ctctcgggat cgagccagcg ccggctgacc accgcctgcg tgcgccgctc ctcgagtggc
     5101 gcatcgtcga gatacgcgta cggccgcgcg gacaggatct cgagcgcgaa cggtgaaggt
     5161 tcgaccaggt cacgtgcgac aacctccaat tcgccgcgct caagcgcgcg cagcagacgc
     5221 tcgaagcctt cgatgtccat ggcctcgtgc aggcagtcgt cgatcgtctg cctgactaag
     5281 ggatgatccg ggatctcgcg atcgccgacg acgttctcct ggcaggcgag ctggtccggg
     5341 aataccgccg cgaccaggtc ctcggcctgc atgcgctgca actgcggcgg gaccttggtg
     5401 ccaccgcgga agcgcggcag cgccagcgcg atcgaggcat cccagcgcca gcgcgtcatg
     5461 aacatgggtg cgtcgagcaa cgcctgcacc agcacctggc gcgcgttgtt cgagtgcaga
     5521 tagcggatca cttcctcgag ttcgaagctg tgcgtggacg tcaacgacag gatgattgca
     5581 tcttcggtcg ccgccgcctg cagctcgaaa ttgaatttgc ggcagaagcg cttgcgcagc
     5641 gccagtcccc aggcgcggtt gatgcggttg ccgtagggcg agtggatgac gagctgggta
     5701 ccgccgcttt cgtcaaagaa gcgctccagc accagccgtt gctgcgtcgg cagcgttccc
     5761 agcacggctc tcgctgtggt gaagtagtcg acgatctgct cggaggcttc ggcagcgacc
     5821 ccgacctcct cgaccagata ggacagcgct gccgcccgat cgtcgagccg cgcttcgagg
     5881 tccgcgcgca accgtgatac cgcggccgag acttcgtcgc tgcgcgccgg cgcctcgccg
     5941 agccagaacg gaatcgacgg cggcatgcct gcggcatctt ccacgcgcac gcggccggcc
     6001 tcgatccgca ggatgcggta cgacgtgttg ccgagctgga acacgtctcc ggccaggctc
     6061 tccaccgcaa agtcctcgtt gacggtgccg atcacgacgt cctgcggcga gagcaccacg
     6121 gtgtaatcgg cggtgtccgg gatcgttcca ccggaagtga cggcggtcag cttcagcccc
     6181 ggccgcggcc gcagcttgcg atttaccgcg tcgcgatgca ggagcgcccg gttgcggccg
     6241 cggcgcatcg agaagccttc ggccagcatt cgtacgatct cgctatattc ctcgcgtgta
     6301 aggtcccggt agggccaggc gcgccgcacc agttcataga gggcgtcctc gtcccattcg
     6361 ccgcccgcca cctcggcagc gatctgctgc gccagcacgt cgaggggctg ccgggggatg
     6421 cgcagccgat ccagctcgcc gcggcgcacg ccgtcgagca gtgcggcgca ttccaccagg
     6481 tcgtcgcgcg acagcggaaa gaggcgccct ttcggcagcc cgccgaccgc gtggcccgag
     6541 cggccgaccc gctgcagcag cgcggagatc gagcgcggcg tggagatctg gcagaccagg
     6601 tcgacgtggc cgatgtctat gccgagctcg agcgatgcgg tggcgaccag cgccttgagc
     6661 tcgccgcgtt tcagtctttg ctccgcattc aatcgctgtt cgcgcgcgag actgccgtgg
     6721 tgcgatgtga cctggtcttc gcccagacgc tccgacagcg cccgcgcaac gcgctcggcc
     6781 agcctgcggg tgttgacgaa taccagcgtc gagcggtgct cgcggatcaa ttcggcaagg
     6841 cggtcgtaga cctgggtcca cacctccgcc gacatgaccg cttcgagcgg cgattccgga
     6901 acctcgatgg cgagatcgcg ccgtcgcacg tggccgctgt cgatgatcgt gcaggcgccc
     6961 ggaccaagca gctgcccgga atgtccgccg ccgacgagaa aacgcgcgac gtcttcgatc
     7021 ggcttctgcg tcgcggacag gccgacgcgg gtcagccgcc cgccggtcag gtgctgcagg
     7081 cgctcgagcg aaagcgcaag atgcacgccg cgcttgttgg gtgccagcgc gtggatttcg
     7141 tccacgatcg cgatgcgtgc ggtcgaaagc atgcgtctgc ccgaatcgct ggtcagcagg
     7201 atgtacagcg actccggcgt ggtcaccagg atgtgcggcg gtttgcgccg catacgctcc
     7261 cggtcggccc gcggcgtgtc cccggtacgc accaatgtgc ggatctccag gtcaggcaga
     7321 ccggcggcgg cgagttcctg ccggatgccg gcaagcggtt cctcgagatt cttccggatg
     7381 tcgttcgaca gcgccttcag cggcgagacg tagacgacgt gagtctcgtc gcgcaattcg
     7441 ccgcgcacac cgagccgcac cagcgcgtcg atcgccgcga ggaaggctgc cagggtcttg
     7501 cccgagccgg tcggcgccgc aatcaggacg tggttgccgg actggatcgc cggccacgcc
     7561 tgcgtctgcg gctcggtcgg tgccgcaaac gctcggccaa accagcgcgc aaccgcagga
     7621 tggaagtttt cgagggcgct cacagattga ttgtccccat ggactttctg caatggctaa
     7681 tccgccagct gaaagtcgaa caatccccgc aatttctcca gataggcaag cgccccgggg
     7741 tcgccttccg ccggcgccca ggacgcgacc tcggccattg agaccggcac ataaggcccc
     7801 gccttcgcct cgaagaacac cgaggctgaa tccagcgcca caagggtgtg gtaaatgccg
     7861 tggggaatgt tcacacccat cgtctcgccg ccggcgcgaa tgatcaggga cttggtcacc
     7921 gtgccgcgtt cgtcgaaaaa caccaccccg aaactgccgc gcacggcaat gatggtttcg
     7981 tccttcgccg ggtccatgtg ccggtgcggg cggacatagg aatcgggctc gacagcgttg
     8041 agcagccggc tcgccggatc cgactcccgc aagtgaaagt tgaaattctt gcgcttgcgc
     8101 gggctctcgc gggcctgccc ccatatctgg tcgagcagcg ccgggccgat gacggtcggt
     8161 tcgttcatgg gtaaacgatc tggacgttct tcggctcgat ccagttgccg agcgaattga
     8221 tcagtccgtc atccagtttt acccgccagg catcgcccaa ctcgatttcg gcgaccgcag
     8281 cgtcgttgct gtacaccacc gagaccgggc agccgccatc cccggcgtcg cggtagggcg
     8341 ctaagagttc cttgagcttg ctgccgctcg actggccgtt gcagtgcagc cgcaccttgc
     8401 gcgcaaagcg gctgcgcgcg gcggcaagat cgtagacccg gtccgcggtt aggcgcaggc
     8461 cctgggtgaa attgtcgaag cttgccttgc cctcgacgat cagcaactga tcctccttga
     8521 gccagttgcg gtggctctcg aacagttcgt tgaataccgt gagctcaacg cgcgctgtac
     8581 cgtcgtcgag cacgaccact gccatgcgcc cgcgccgggt ctgctgctgc cggatgctgt
     8641 agatcacgcc cgcgacgatc accgtctgtc cctgcggctg gatattggcg agccgagtcc
     8701 tggcaaaggc ctccacttcc gcggcgtagc tttggaaagg gtggccgctg aagtaatagc
     8761 cgagcgcgag tttctcgttc tgcagttgct ctttcgcctc ccagcgcgcc gcttcgatca
     8821 gcttcggccc ttcggccgtt gccgacgcat cgccgaagag gctgttctgc gaggcggccc
     8881 ggttctcctg ctcggccgcc tccatggcga tgccgacgga ggccatcagg ctggcgcggt
     8941 gatcgttaat ggaatcgaac gctccggcac gcaccaaaga ttcgatcacc cggcgattga
     9001 cgacacgctt gtccaccctg cggcagaaat cgaacaggtc cttgaaagat ccgccctgct
     9061 ggcgcgcctc gatgatgtgg gtaatcgccg attctccggt ccctttcacc gcccccagcc
     9121 catagcggat gcgcttggca tccaccggca cgaagcggta ctcggccgcg tgaatatccg
     9181 gtggcagcac ttccaggccg ttggcaatcg tatcctcgca gaactgctgc accttgtcgg
     9241 tatcgtccat gaccgccgac atgttggcag ccatgaacgc cgccgcgtga tgcgccttga
     9301 aatacgccgt ctggtaggcg accaaggcgt aggctgcggc atgcgatttg ttgaagccgt
     9361 agccggcgaa cttctccatc aggtcgaaca actgctcggc gcggtgctcg gacacaccgt
     9421 tcttcatcgc gccggccttg aagatgtcgc ggtgctgcgc catttcctcg ggtttcttct
     9481 tgcccatcgc gcggcgtagc aggtcggcgg caccgaggct gtaaccgccg atcacctggg
     9541 cgatttgcat cacctgttcc tggtacacca tgatgccgta ggtcggcgac aggatcgtct
     9601 ccaggcgctt gtcgagatac tcgaagcgct cctggccctg cttgcgcttg acgaagtcgg
     9661 ggatgagctc catcggtccg gggcggaaca aggcgaccag cgcgatgatg tcttcgaagc
     9721 ggtcgggcct ggcctgtttc agcagctcgc gcatgccacg cgattcaaac tggaacaccg
     9781 ccgtggtatt ggcgccggca aacaccttga acgcggccgg gtcgtcgagc ggaagctgct
     9841 cgagccggat ctcggaatcg ggatcgaggc gcttgatgaa gcgcagcgtc cagtcgagga
     9901 tggtaagcgt ggtcaggccg aggaaatcga atttcaccag tccgatcgct tccacgtcgt
     9961 ccttgtcgag ctgcgacacc gcgctgtccg aaccggaagg cgcatataac gggcagaagt
    10021 cggtgagctt gccgggcgcg atcagcacgc cgccggcatg catgccgaca ttgcgggtca
    10081 agccttcgag ttgttccgcg agcgccagca gctcgcgcgt ttcctcctcc gattcctcgc
    10141 gctccttcaa ccgtggctcc atctcgcgcg catccttgag cgtgacgagc ttgcccggct
    10201 ggaacgggat cagcttggcg agctcgtcgg tgcggctgta gttccattcc atgacgcggc
    10261 ccacatcgcg caccaccgcc ttcgccgcca tcgtgccaaa ggtcacgatc tgcgacaccg
    10321 actcctcgcc gtacttgtgg cggacatact cgatcacgcg gtcgcggccg tcctggcaga
    10381 agtccacgtc gaaatccggc atcgataccc gctcggggtt gaggaagcgt tcgaacagca
    10441 ggtcgtagcg caacggatcg aggtcggtga tgcccagcga atacgcgacc agcgagcccg
    10501 cgccggaacc gcggcccggc cccaccggta taccgttcgc cttggcccaa ttgatgaaat
    10561 cggcgacgat gagaaagtag cccgggaagc ccatgtgcac gatggtcttg atctcgaact
    10621 caaggcgttc gcgataacgc ggcacgtgct cggcgcgtgc ctccgggtag agcgcctcca
    10681 tgcgcagttc cagaccggca accgagcgct catgcagata ctgctcgaga ctgactccct
    10741 cgggagtggg aaacagcggt agcctcgact tgccgagatc gagcgggaaa ttgcagcggc
    10801 gggcaatctc caccgagtta tccagcgcct gcggcaggtc ggcgaagagt tccgtcatct
    10861 cggcctgggt cttgaaatac tgctcttcgg taaagtggcg cgggcggcgc tggtcggaaa
    10921 ggacataccc ctcggcaatg cacactcgcg cctcgtgggc gacgaaatcc tcgcgcttga
    10981 gaaactgcac cggatgcgtc gcgaccaccg gcagtttcag gccggaagca agacgtaccg
    11041 cgcgcttgac gtagtgctcg gcatgggcat gaccggcgcg ttgcaactcg atatagaagc
    11101 gcttcgggaa aagctgcgcc cagtcctgtg ccagcttctg cgcctgctgc gggttatcgg
    11161 caagcagcgc atggccgata tcgccgagat gcgcgcccga cagcacgatc aatccttcgg
    11221 tgccggtctc gctgagccat ttcttgtgga tttcggcgcg gccgcggtgc tgattgttgc
    11281 ggtaggcgcg cgtcagccag tcgcacagcc gcaggtagcc cgcggaagag gcacacagca
    11341 gcaacaccct gaacggcttg tcacggtcgg cgtcgttgct gatccagacg tcgcagccca
    11401 tgatcggctt gagtccctgg ctgcgcgccg cctggtagaa cttcaccatg ccgaagacgt
    11461 tggagaggtc ggtcaacgcc agcgcgggca tcgaatccgc ctttgctgtc gccactgcgc
    11521 cgtcgagccg ggcgatgccg tcaacgatcg aatattcgct gtgcaagcgc agatggatga
    11581 acttcggttg catg
//
LOCUS       DASQOP010000131         3324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2870592, whole genome shotgun sequence.
ACCESSION   DASQOP010000131 DASQOP010000000
VERSION     DASQOP010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2870592"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VE030_05340"
     CDS             complement(<1..248)
                     /locus_tag="VE030_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HZF22876.1"
                     /translation="MRFRRGNRIRLLRNVEEYLPALEREIDAATSEIFLETYIFEAGD
                     TERRVTAALVRAARRGVLVHLMIDGFGSRLFPEDQRRQL"
     gene            complement(245..997)
                     /locus_tag="VE030_05345"
     CDS             complement(245..997)
                     /locus_tag="VE030_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HZF22877.1"
                     /translation="METLHVVTYNIHKGFSQFKRRMAVHDLRDKLRNIGADLVFLQEV
                     QGLHERHAERHHNWPVSPQYEFLADSIWNDFAYGRNAVYDSGHHGNAILSKYPIVRWD
                     NEDVSMHRAERRGLLHCEIAIPGWSQPLHCVNVHLGLLGHWRKKQIVLLKTRIERLVP
                     PEAPLVVAGDFNDWRRRASHALEQSLNVSEVFETTAGRPARSYPALLPFLHLDRIYVR
                     GFGIRIAHAHRGRAWSRVSDHIALSALLTRKA"
     gene            complement(1115..1684)
                     /locus_tag="VE030_05350"
     CDS             complement(1115..1684)
                     /locus_tag="VE030_05350"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZF22878.1"
                     /translation="MHHLLWLALVFPALCHAADISLRAARYGGTFEVEAAAEFEAEVT
                     VAWAVLTDYDRLSEFIPGMHSSRIVSRSKRRVIVDQSGEARLLFLSFPIQVRLEVEEF
                     PYQRIVSRAIEGNFKDLRGAYTLEAHGRRLLLHYTGSLTPDFSIPPLIGTILVRNTVE
                     KRFGAMVDEIMRRQRLRELPVTKSSGSGG"
     gene            complement(1744..2841)
                     /gene="nadA"
                     /locus_tag="VE030_05355"
     CDS             complement(1744..2841)
                     /gene="nadA"
                     /locus_tag="VE030_05355"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006883.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HZF22879.1"
                     /translation="MQASATAFEQYSRLQDDDCEARIVAAKIKLGKRAALLAHHYQRA
                     DVYKHADLTGDSLKLSKLASQTDAEFIVFCGVHFMAEVADILCKSGQITVLPDMAAGC
                     SMADMANVSKVERAWRELGEVLDPDQHVTPATYINSAADLKAFCGEHGGIVCTSSNAK
                     QILDWSFARREKVLFFPDQHLGRWTGHKMGIPLEEMVVWDPDEPMGGLSVEQIKAARL
                     LLWKGHCSVHQMFQPQHILKFRNQHPDGLVISHPECKFEVCKLSDYVGSTETIINTIR
                     NAPADTRWLVGTELNLVNRIAEEFKPQGKQIQFMAPLVCMCSTMARIDPQHLAWTLEN
                     LTEGRVVNRIKVPAHDAELAGVALDRMLAVS"
     gene            complement(2914..>3324)
                     /gene="nudB"
                     /locus_tag="VE030_05360"
     CDS             complement(2914..>3324)
                     /gene="nudB"
                     /locus_tag="VE030_05360"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HZF22880.1"
                     /translation="PDLQVLLLERADHPGFWQSVTGSQDEGETLRQTAAREVAEETGL
                     DAEAFGLTDWNMRNEYEIYPEWRWRYAPGVTRNVEHVFGLRLPGALPVKVAPGEHLGY
                     VWMPHDRAADKVFSWSNAQVLRTLPEHLSNRLQQ"
BASE COUNT          587 a         1049 c         1068 g          620 t
ORIGIN      
        1 agttgccggc gctggtcttc gggaaacagg cgcgatccga agccgtcgat catcaggtgc
       61 accagcaccc cgcgccgcgc cgcgcgcacc agcgctgcgg tgacgcgccg ttcggtgtcg
      121 ccagcctcga atatgtacgt ctccaggaaa atttcgcttg tcgcagcgtc gatctcgcgc
      181 tcgagcgccg ggaggtattc ctcgacgttg cgcagcagcc ggatccggtt tccgcgccgg
      241 aatctcatgc cttccgggtc agcaacgccg acaacgcgat gtgatcggag acccgggacc
      301 aggcgcggcc acggtgcgca tgagcgatgc ggatcccgaa gccgcgcacg taaatgcggt
      361 ccagatgcag gaacggcagc agtgcgggat aactgcgcgc ggggcggccc gccgtcgtct
      421 cgaacacttc ggagacgttg agggactgtt ccagcgcatg gctcgcgcgt ctgcgccagt
      481 cgttgaaatc gccggcaacg accaacgggg cctcgggcgg caccaggcgt tcgattcggg
      541 tcttgagaag cacgatttgt ttcttgcgcc agtggccgag caatccgagg tgcacgttga
      601 cgcaatgcaa gggctggctc cagccgggaa tggcgatctc gcaatggagc aggccgcggc
      661 gttcggcgcg atgcatcgaa acgtcctcgt tgtcccatct gacgatcggg tacttgctca
      721 ggatggcgtt gccgtgatgg ccgctgtcat agaccgcgtt cctgccgtag gcaaaatcgt
      781 tccaaatcga atcggccagg aattcgtact gcgggctgac gggccagttg tggtggcgct
      841 cggcgtggcg ttcgtgcaag ccctggacct cctgcaaaaa caccaggtcc gcgccgatgt
      901 tgcgcagctt gtcgcgcagg tcgtgaacag ccatgcgccg cttgaactgc gagaagcctt
      961 tgtggatgtt gtaagtgacg acgtgcagcg tttccatgga tgccctgctg ttgagcccga
     1021 tgccgcgacc cgccggccca agattcaatc gccggcgtcg aacgcaatat tacctgcgga
     1081 cggactgcgc cgccgcactc ggccctggcc gcgcctagcc accactgccg gaggatttgg
     1141 tcacgggaag ttcgcgcagg cgctgccggc gcatgatttc gtccaccatc gcgccgaagc
     1201 gcttttccac ggtgttgcgc accagaatgg taccgatcag cggcgggatg ctgaagtccg
     1261 gcgtcagcga gccggtgtag tgcagcagca agcggcgccc gtgtgcttcg agagtgtaag
     1321 ccccgcgcag gtccttgaag ttgccctcga ttgcgcgcga gacgatgcgc tggtagggaa
     1381 actcctcgac ttcgagccgc acctggatgg gaaaggagag aaacagcagc ctggcttcgc
     1441 cgctttggtc gacgatgact ctgcgtttgc tgcgggagac gatccggctc gagtgcatgc
     1501 cggggatgaa ttccgacagc cggtcgtagt cggtcaggac agcccaggcc acggtcactt
     1561 ccgcctcgaa ctcggcggcc gcctcgactt cgaaggtgcc gccataacga gccgcccgca
     1621 ggctgatgtc ggccgcgtga cacagggccg gaaataccag cgcaagccac aggaggtgat
     1681 gcatcgtcgg cctgcggcgc ctctactgaa atcgttgccc ccgccaggcg gagccggcgc
     1741 ggttcaggac accgcgagca tgcggtcgag cgcaaccccg gccaactcgg cgtcgtgcgc
     1801 cggcaccttg atccggttca ccacccttcc ctccgtcagg ttttccaacg tccaggccag
     1861 gtgttgcggg tcgatgcgcg ccatggtcga gcacatgcac accagcggcg ccatgaactg
     1921 gatctgcttt ccctgcggct tgaactcctc ggcaatgcga ttgacaagat tcagctccgt
     1981 gccgaccagc cagcgggtgt ccgccggcgc gttgcggatg gtgttgatga tcgtttcggt
     2041 cgagcccacg tagtccgaga gcttgcacac ctcgaatttg cattcggggt gcgaaattac
     2101 cagcccgtcc ggatgctgat tgcggaactt gagaatgtgc tgcggctgga acatttgatg
     2161 taccgaacag tgtcctttcc acagaagcag gcgggcagct ttgatctgct cgaccgacag
     2221 gccgcccatc ggttcgtccg ggtcccaaac caccatttcc tcgagcggga tgcccatctt
     2281 gtggccggtc cagcggccca ggtgctggtc gggaaagaac agcaccttct cgcggcgcgc
     2341 aaacgaccaa tccaggatct gtttcgcgtt cgacgacgtg cagacgatgc cgccgtgctc
     2401 gccgcagaaa gccttcagat cggcagccga gttgatgtac gtggccggcg tgacgtgctg
     2461 gtcgggatcg agcacttcgc ccagttcgcg ccaggcgcgc tcgactttcg acacgttggc
     2521 catgtccgcc atcgagcaac cggcggccat gtcggggagc acggtgatct ggccggattt
     2581 gcacaggatg tccgcgactt cggccatgaa gtgcacgccg cagaacacga tgaactcggc
     2641 gtcggtttgc gaggccagct tggagagctt gagtgagtcg ccggtcaggt ctgcgtgctt
     2701 gtagacatcg gcgcgctggt aatggtgggc gagaagagct gcacgcttgc cgagctttat
     2761 cttggccgca acaatgcgtg cctcgcagtc gtcatcctgc aggcggctgt actgctcaaa
     2821 agcggtcgcg ctggcttgca tgctgtgcgg cccctcatcg gccccggtaa atcagtagcg
     2881 aaaaccttat cacactagga ccgcaaatcg cgatcattgt tgcagccggt ttgacaggtg
     2941 ttccggcaac gtcctgagaa cctgcgcgtt gctccaggaa aagaccttgt ccgcggctcg
     3001 gtcatgcggc atccagacgt agccaagatg ctcgcctggc gcaactttta ccggcagcgc
     3061 cccgggcagg cgcaagccga acacatgctc gacgttgcgg gtgacgcccg gtgcatagcg
     3121 ccagcgccac tccggataga tctcgtactc gttgcgcatg ttccagtcgg tcagcccgaa
     3181 ggcctcggca tccaggccgg tctcttccgc cacttcccgc gcagcggtct ggcgcagcgt
     3241 ctcgccctcg tcctggctgc cggtgaccga ctgccagaag cccgggtgat ccgcgcgctc
     3301 cagcagcagc acctgcaggt cggg
//
LOCUS       DASQOP010000132         3487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1862173, whole genome shotgun sequence.
ACCESSION   DASQOP010000132 DASQOP010000000
VERSION     DASQOP010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1862173"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VE030_05365"
     CDS             complement(<1..450)
                     /locus_tag="VE030_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZF22881.1"
                     /translation="MPELRRMLPSSNALFVFDAAARNGSFTAAAAELNVTQPAVSRML
                     GRFEQYLGVRLFDRKAGGAILTEEGELLYRCVLDGFRSIESGLIEIERRRRGTETVTL
                     SLSSAFTTHWLMPRMDKLQRRFPNVDLRFQLISGSLRGPVENVDFGMR"
     gene            705..1787
                     /locus_tag="VE030_05370"
     CDS             705..1787
                     /locus_tag="VE030_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZF22882.1"
                     /translation="MGTGGESVEIRSASKSYGAVRALDNVTLNVAPREFVSLLGPSGS
                     GKTTLLGLLGGFIQPSSGSIHFGDRDVTLEPPHKRDIGIVFQNYALFPHMNVSENVAF
                     PLRARRLPKASWSEKVRTALAMVGLAGYEERGVAQLSGGQRQRVALARAIIFEPRLIL
                     MDEPLSALDKQLRESMQIELRELHKRIGATIIYVTHDQREALTMSDRVAVLKGGRIVQ
                     IDRPERLHDHPADSFVASFIGEASLLPVRRIDSGSVALGPAILRSARTVPNGDALILA
                     VHSEKLLIDDGAADAARNRLTGTVTDVVYQGESLRIFLALPDGTALSLRQPSHHEAYR
                     RIPRLGGSLTVTLHPEDTIIVPKAPE"
     gene            1832..2530
                     /locus_tag="VE030_05375"
     CDS             1832..2530
                     /locus_tag="VE030_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZF22883.1"
                     /translation="MSLSNFKVLTFDVVGTLIDFETGVLSAVRKISGRSVAELSDEQI
                     FASYKRGRDAHPERSSEVMFDVYRFLAKELGLPADDLACDAFQLAVLRWQPFADSVEA
                     LKRLRTKFRLVAMTNADRVALSCYAHALGDPFDDTVCADDTGVAKPNPEFFAYNKGRQ
                     AAFGYKQFDILHVAQSQYHDIGIARKLGYKVCWIERRQGMAGFGGTPDVPALTKPDFH
                     FATMKAFADAAVGH"
     gene            2550..>3487
                     /locus_tag="VE030_05380"
     CDS             2550..>3487
                     /locus_tag="VE030_05380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZF22884.1"
                     /translation="MAQVWTPLASATSLWASTAEPLRAFPSLSSEVQADVVIIGAGYT
                     GLSAAHHIAKSGLTPIILEANRPGWGASGRNGGVITAKFRQSFRGIDAAHGRAMAKRM
                     YEIAHESTEIVEELVSEFGIVGARLTRSGQVKAAHNHATLRAAVDEADWMKREMGDAD
                     VRILNADQVREETGSQAFVGGVLNPGSGGIHPLNYLHGLAEGLAQRGVAIFQDSPALR
                     LRRENGLIFAETPQGAVRARQVIIATNSYSDLTPATRHMQHTLVPFRSAIIATDKLPH
                     NLAGHLMPTGRTYTETKRMMRWFRMVDDRVVFGGRGA"
BASE COUNT          678 a         1113 c         1073 g          623 t
ORIGIN      
        1 tcgcataccg aagtccacgt tctcgaccgg gcctcgcaat gagccggata tcagctggaa
       61 ccgcagatca acattcggaa accggcgctg cagcttgtcc atgcgtggca tcagccagtg
      121 cgtggtgaag gccgaggaga gcgagagcgt gacggtttca gtaccccggc ggcggcgctc
      181 gatctcgatc aggccgcttt cgatgctgcg aaatccgtcg aggacacacc ggtagagtaa
      241 ctcaccctcc tccgtcagaa tcgcaccacc cgccttgcga tcaaaaagac gtacgccgag
      301 atattgttcg aaacgtccca acatgcggct aaccgccggc tgggtcacat tcagttcggc
      361 cgccgccgcc gtgaagctgc cgttgcgtgc agccgcatcg aagacgaaca gcgcattgct
      421 ggaaggcagc atccggcgaa gctcaggcat aacatcagat tatgatgcgg gtgataattt
      481 ggcaattgct ccgctcaacg aattttggtt gcatgatgaa agggaaagcc tgccgggaag
      541 cggagtgccc tgatcaacct gcggcagggg aagctctgtg tcgcgcagga tgcgtccgcc
      601 tataaattga gatcatgcca attcaactgc ccctggccta ggcgagggcg tcggggatcc
      661 gcgttggtgc gaccatcggc gaggagcaac aggaaggtcg ttcgttgggc accggcggcg
      721 aaagcgtcga aatcagatct gcgagcaaga gctacggcgc tgtccgtgcg ctcgacaacg
      781 tcacgctcaa tgtcgcaccg cgtgaattcg tctctcttct ggggccatcc ggatccggga
      841 agacgacgct tctcggctta ctcggcggat tcatccagcc ctcgtcgggc tcgattcatt
      901 tcggcgaccg cgacgtcacc ctggagcccc cgcacaagcg cgacatcgga atcgtgttcc
      961 aaaactatgc gctgtttccg catatgaacg tcagcgagaa cgttgccttc ccgctgcgcg
     1021 cgcgacggct tcccaaggcg agttggtcgg agaaggtgcg tactgcactg gcgatggtcg
     1081 gcctcgcggg ctacgaggag cgcggcgtgg cccagctctc cggcggccag cgccagcgcg
     1141 ttgcgctcgc aagggcgatc atttttgagc cgcgactgat tctgatggac gagccgcttt
     1201 ccgcgctcga caagcagctg cgggaatcga tgcaaatcga gctgcgcgaa ctccacaagc
     1261 gcattggcgc caccatcatc tatgtcacgc atgaccagcg tgaggcactg acgatgagcg
     1321 accgggtcgc cgtcctgaaa ggcgggcgca tcgtccagat cgaccggccg gagcgcctgc
     1381 acgatcatcc ggcggattcc ttcgtggcca gcttcattgg cgaggcgagc ctgctgcccg
     1441 tccgccgcat cgactccggc agcgtggcgc tgggacccgc catcctgcgc agcgcccgga
     1501 ccgtcccgaa cggcgacgcg ctgatcctgg cggtacatag cgaaaaactg ctgatcgacg
     1561 acggcgcagc cgacgcagcg cgcaaccgcc tgaccggcac cgtcacggac gtcgtctacc
     1621 aaggcgaaag cctgcgcatc tttctcgcgc tcccggatgg caccgcattg agcctgcgcc
     1681 agccgagcca ccacgaagcc tatcgccgca ttcctcgact tggcggcagc ctcacggtga
     1741 ccctccaccc ggaggacacg atcatcgtgc cgaaggcacc cgaatgaagg tccatcacca
     1801 gcacagtccc agcaccagag aagaaacgcc catgtcgctc agcaatttca aggttctcac
     1861 ttttgacgtc gtcggcaccc tgatcgactt cgaaaccggc gtgctcagcg ccgtccgcaa
     1921 gatctccggc cgaagcgtgg cagaactcag cgatgaacag atcttcgcat cctacaagcg
     1981 cggccgcgat gcgcatccgg agcgatccag tgaagtgatg tttgatgtct atcgcttcct
     2041 ggcgaaggaa ctcggcttgc cggctgatga tctggcctgc gacgctttcc agcttgcggt
     2101 gctgcgctgg cagccgttcg ccgactcggt cgaggcgctg aaacgcttgc gcacgaaatt
     2161 tcggctggtc gcgatgacca atgcggatcg cgtcgcgctg tcgtgctacg cgcacgccct
     2221 gggcgacccg ttcgacgata ccgtttgcgc cgacgatacc ggcgtcgcca aaccgaatcc
     2281 cgaattcttt gcctacaaca agggcaggca ggcagccttc ggctacaagc agttcgatat
     2341 tctgcatgtg gctcagagcc agtaccacga tatcgggatc gctcgtaagc tcggctacaa
     2401 ggtgtgctgg atcgaacgcc gccagggcat ggcgggattc ggtggaacgc ccgacgtacc
     2461 ggcgctgacc aagcctgatt ttcatttcgc aacgatgaag gccttcgccg acgcggcggt
     2521 tgggcactga acggagcgag tgcgcggcca tggcacaggt ctggacccca ttggcatcgg
     2581 ctacctcgct ctgggcgagc acagccgagc ccttgcgtgc ctttccttct ctgtcgagcg
     2641 aggttcaggc cgatgtcgtc atcatcggcg ccggctatac cgggctctcc gccgcgcatc
     2701 acatcgccaa gagcggactg acgccgatca tccttgaggc caatcgtccc ggctgggggg
     2761 cgagcggccg caatggcggc gtcatcaccg cgaaatttcg tcagtccttc cgcggcatcg
     2821 acgcggccca cggccgcgcg atggcgaaac gcatgtacga gatcgcgcat gaatcgacag
     2881 agatagtcga ggaactggtt tccgaatttg gcattgtcgg cgcgcggttg acgcgaagcg
     2941 ggcaggtcaa ggcggcacac aatcacgcga cgcttcgagc cgcggttgac gaagccgatt
     3001 ggatgaagcg tgagatgggc gatgcggatg tccgaatact caacgccgat caggtgcgcg
     3061 aggaaaccgg ctcgcaggcg tttgtcggcg gcgtgctcaa tccgggttcc ggcggcatcc
     3121 atccgctgaa ttatttgcat ggccttgccg aaggcctggc gcaacgcggc gtcgccatct
     3181 tccaggacag ccccgcgctc cggttacggc gcgaaaatgg gctgatcttc gccgagacgc
     3241 cgcaaggcgc ggtgcgggcg cggcaggtca tcatcgcgac caacagctat tccgacctga
     3301 cgcctgcaac gcgtcatatg cagcacacct tggtgccgtt ccgcagcgcc atcatcgcaa
     3361 ccgacaagct gccgcataac cttgccggcc acctgatgcc gaccggccga acctataccg
     3421 agaccaagcg catgatgcgc tggttccgca tggtcgacga tcgcgtggtc ttcggcggcc
     3481 gcggcgc
//
LOCUS       DASQOP010000133         3377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_252147, whole genome shotgun sequence.
ACCESSION   DASQOP010000133 DASQOP010000000
VERSION     DASQOP010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252147"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VE030_05385"
     CDS             <1..531
                     /locus_tag="VE030_05385"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HZF22885.1"
                     /translation="PEQIQAANKATVEALLAVANTQFTALEKLATIQASAVKSAFEDS
                     VANARALLGAKDVQEFVTLQNSFTQPAIEKAIAYSKSVYEVATEANAELSKVAERRVA
                     EWNENFVTLLDKVSKNAPAGSDVAVAAVKSMLAAANSAYDNLSKAAKQATEIAEANVA
                     AATETVKGLAKPKKAA"
     gene            complement(641..1381)
                     /locus_tag="VE030_05390"
     CDS             complement(641..1381)
                     /locus_tag="VE030_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HZF22886.1"
                     /translation="MAIGAFIIGDEMLVGKRQDKHLAFLIGALTRRGLRLAWAQYLGD
                     DPERLTAALSHSFASGDVVFSFGGIGATPDDHTRECAAAAARLALRLHPDAEREIRAR
                     FGGDATPQRLKMGEFPEGARIIPNPVNRIPGFSLREHHFVPGFPQMAAPMVEWVLDTQ
                     YQHLFDRDRWAERSILVYEAGESQLIPAMEAVGARFPDVKVFSLPSMGEDGSRIHVEL
                     GVRGNPARVGDAIEALCHAVSKGGFPYK"
     gene            complement(1383..2120)
                     /locus_tag="VE030_05395"
     CDS             complement(1383..2120)
                     /locus_tag="VE030_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HZF22887.1"
                     /translation="MFANREGQRVPQVTFRIRAANEWESVTSDDIFNGKTVVVFSLPG
                     AFTPTCSSVHVPRYNELAPAFYANGVDKIVCVSVNDPFVMSEWAAAQEAGNITMLPDG
                     NGEFTEKMGLLVDKSELGFGKRSWRYSMLVKDGVIQKMFIEPQKPGDPYEVSDADTML
                     NYVNPKAKKPDQVAILTREGCDYCAKAKVLLKDLGYDYAEVPLDHRIRTRVIGAITGR
                     GTVPQVFINGKHIGGLEDLQQWAKKAA"
     gene            complement(2223..2558)
                     /locus_tag="VE030_05400"
     CDS             complement(2223..2558)
                     /locus_tag="VE030_05400"
                     /inference="COORDINATES: protein motif:HMM:NF018917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HZF22888.1"
                     /translation="MERLDRAFGMLGLGILTLPLWLLPPLWPLIPVAIFGWGNQRLLR
                     YDALAEHADRAEMARIFRERRGGLYLLGLQLALLAYVPVLGFFAPVVFGLAFIYYLLG
                     ALQAARRHD"
     gene            complement(2471..2941)
                     /locus_tag="VE030_05405"
     CDS             complement(2471..2941)
                     /locus_tag="VE030_05405"
                     /inference="COORDINATES: protein motif:HMM:NF018917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HZF22889.1"
                     /translation="MSIARSLLYGVANLLHPRMLWLMLWPMLIALAIWGTVAIALWVR
                     VATLIAGVLQRWLESAMFFANFNLGDVTLIAAHVLLFLLFVPLVYLTALFILGIFGMQ
                     AMVDHVARRSFPDLERRRGGGVAGSGWNGLIALLGCSALASLPCRSGCCRRCGP"
     gene            complement(2938..3330)
                     /locus_tag="VE030_05410"
                     /pseudo
     CDS             complement(2938..3330)
                     /locus_tag="VE030_05410"
                     /inference="COORDINATES: protein motif:HMM:NF023326.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
BASE COUNT          698 a         1074 c         1047 g          558 t
ORIGIN      
        1 ccggaacaga ttcaagcggc caacaaggcc actgtagaag cacttcttgc cgtcgccaac
       61 acgcagttca ctgcattgga aaagctggcg actatccagg caagcgccgt aaagagtgcg
      121 ttcgaagaca gcgttgccaa cgcccgtgcg ctgctcggcg cgaaggatgt gcaggagttc
      181 gttacgctgc agaactcatt tacccagccc gccatcgaga aggcaatcgc ctactcgaag
      241 agcgtctacg aagtcgcgac cgaggcgaat gccgagttgt cgaaagtggc ggagcgccgt
      301 gtcgccgagt ggaatgagaa cttcgtgacg ttgctcgaca aggtctcgaa gaacgcgccg
      361 gccgggtcgg acgtcgcggt cgccgcggtg aagtcgatgc tcgccgccgc gaattcggcc
      421 tacgacaacc tcagcaaggc agcgaagcaa gcgaccgaga tcgccgaggc gaacgttgct
      481 gccgcaaccg agactgtaaa gggcctcgcg aagccaaaaa aggccgcgta agcgccaaac
      541 cgggcaagag caacaacgcc cgatctagcg cgaagcgcag ccgcacaaag gctgccaggt
      601 ccaaaaagaa gtaaggaagc cccgcttcaa gcggggcttt tcatttgtag gggaaaccgc
      661 cctttgatac cgcgtggcac agtgcttcga tcgcgtcgcc gactcgggca gggttgcccc
      721 gcacgcccag ttcgacatga attcggcttc cgtcctcccc catcgagggc aggctgaaca
      781 ccttcacatc cggaaagcgc gcccccactg cttccatcgc cgggatcagc tggctctcgc
      841 cagcctcgta aaccaggatt gatctttccg cccagcggtc gcggtcaaac aggtgctggt
      901 actgggtatc aagcacccat tcgaccattg gcgctgccat ctgcggaaag cccggtacga
      961 aatggtgctc gcgcagcgag aatcctggga tccgattcac cggattggga atgatccgcg
     1021 cgccttctgg gaattcgccc atcttcagcc gctgcggagt cgcgtcgcca ccgaagcgcg
     1081 cacggatctc ccgctcggca tccggatgca ggcgcaacgc caaacgcgcc gccgccgccg
     1141 cacactcgcg tgtgtgatcg tcaggcgtcg ccccgatccc cccgaagctg aagacgacgt
     1201 cgcccgaggc gaaactgtga ctgagcgccg ccgttaggcg ctcggggtcg tcgccgagat
     1261 attgcgccca ggcaagccgc agcccgcgcc ttgtgagggc gccaatgagg aaggcgagat
     1321 gtttgtcctg acgctttccc accagcattt catccccgat gatgaacgcg ccgattgcca
     1381 aatcacgctg cctttttggc ccattgctgc agatcctcca ggccgccgat atgcttgccg
     1441 ttgataaata cctgcggcac ggtgccgcgg ccagtgatgg cgccgatcac gcgcgtgcgg
     1501 attcggtggt caagcggtac ctcggcgtaa tcgtagccaa gatccttgag tagaaccttc
     1561 gctttcgcgc agtagtcgca gccttcgcgc gtgagaatcg ccacctgatc tggcttcttc
     1621 gccttgggat tcacgtagtt cagcatggtg tcggcgtccg acacctcgta ggggtcgcct
     1681 ggcttttgcg gctcgatgaa catcttctgg atcacgccgt ccttcaccag catcgaatag
     1741 cgccatgagc gcttgccgaa gccgagctcg gacttgtcta ccagcaatcc catcttctcc
     1801 gtgaactcgc cgttcccgtc cggcagcatt gtgatgttgc ccgcctcctg cgcggccgcc
     1861 cactcgctca tgacgaatgg atcattgacc gagacgcaga cgatcttgtc cacgccgtta
     1921 gcgtagaacg ccggcgccag ctcgttatag cgcggcacgt gcacggacga gcaggtcggc
     1981 gtgaacgccc cgggtagaga aaataccacc acggtcttgc cgttgaaaat gtcatcggag
     2041 gtgacgcttt cccactcgtt ggcggcgcgg atgcggaacg tgacctgagg aactctttgt
     2101 ccttcgcggt tggcgaacat gatctgactc ctggctctgg gattcaaaat ggaaagacgg
     2161 agtctaggaa agcgcgttcc gggtggccaa tggtttgttc ggatggcggc aatagctgca
     2221 gcctaatcgt ggcgccgcgc ggcctgcagg gcgccgagca ggtagtaaat gaaggcgagc
     2281 ccgaaaacca ccggcgcgaa aaagccgagg accggtacgt aggccagcag cgccaactgg
     2341 aggccgagca ggtagaggcc gccgcgccgc tcgcggaaga tccgcgccat ctcggcccgg
     2401 tcggcatgct cggcaagggc atcatagcgc agcagccttt gattgcccca gccgaagatc
     2461 gccaccggga tcaggggcca cagcggcggc agcagccaga gcggcagggt aaggatgcca
     2521 aggccgagca tcccaaaagc gcgatcaagc cgttccaccc gctccccgcg accccgccgc
     2581 cgcggcgccg ctccagatcg gggaaggagc ggcgcgccac gtgatcgacc attgcttgca
     2641 tgccgaagat gccgaggatg aataaagcag tgaggtaaac cagcggcacg aacaggagga
     2701 acagcagcac atgcgcggcg atgagggtga cgtcgccgag gttgaagttc gcgaagaaca
     2761 tcgccgattc gagccagcgc tgcagcaccc cggcaatcag ggtcgcaaca cgcacccaca
     2821 gcgcgatcgc cacagtgccc cagatggcga gcgcgatcag catcggccac aacatcagcc
     2881 agagcatgcg gggatgcagc agattggcga cgccgtacaa caacgagcgc gcgatgctca
     2941 tttgcgcaga gtctgccaga gttttgccag gcgcttgacc gacacaggga ccggggtgcg
     3001 cagctcctgt gcgaagagcg aaacacgcag ctcctcaatc agccagccga actgctcgat
     3061 ctgcagagtg accgcgccct gccgcaagcg cgtggcaagc tcccgccgct aaggctgctc
     3121 gacggacgcc agctccacgg ccagacgcgc atcgcgcgct ggatcggcgc gcagcttgtc
     3181 gacgcgtaat gacgccgctt tcacatagcg cggcaggtgc tgcaagcgct cccatggagt
     3241 gcgagcgagc cagccgggcg cgaggagccg gccaagctgc tgtttaatat catcaccgac
     3301 ctgcgggagt gccttcagtg cggggggcaa ttttttttcc agtgccgcgc gctcggccag
     3361 gatgcccgag gcggcgc
//
LOCUS       DASQOP010000134        14902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_654579, whole genome shotgun sequence.
ACCESSION   DASQOP010000134 DASQOP010000000
VERSION     DASQOP010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14902
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654579"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..119)
                     /locus_tag="VE030_05415"
     CDS             complement(<1..119)
                     /locus_tag="VE030_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HZF22890.1"
                     /translation="MEVNAMSRSLQGTGASRRLRRTGKVPGIVYGGEQPAQNI"
     gene            complement(275..1225)
                     /locus_tag="VE030_05420"
     CDS             complement(275..1225)
                     /locus_tag="VE030_05420"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HZF22891.1"
                     /translation="MAYDSLMVFTGNANPKLAEDVVKHLNIPLGRATVGRFSDGEVMV
                     ELLENVRGKDVFVLQSTCTPTNDSLMEVMLMVDALKRSSAGRITTAIPYLGYSRQDRR
                     PRSARVAISAKVVANMLVVAGVDRLLTMDLHADQIQGFFDIPVDNIYSAPILLGDIWK
                     RGHQDLVVVSPDVGGVVRARALAKRLESDLAIIDKRRPRPNVATVMNIIGDVADRTCV
                     IMDDMVDTANTLCEGALALKEAGAVKVLAYCTHPVLSGKAIERIENSVLDEVVVTDTI
                     PLREEARCCKRIRQLSIAALLAETMLRISNEDSVSSLFME"
     gene            complement(1358..1432)
                     /locus_tag="VE030_05425"
     tRNA            complement(1358..1432)
                     /locus_tag="VE030_05425"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1398..1400),aa:Gln,seq:ttg)
     gene            complement(1439..2308)
                     /gene="ispE"
                     /locus_tag="VE030_05430"
     CDS             complement(1439..2308)
                     /gene="ispE"
                     /locus_tag="VE030_05430"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HZF22892.1"
                     /translation="MSLAVKSGAAGTAAARFAAPAKLNLFLHVVGRREDGYHLLQTVF
                     RFIDYGDELGFTVRDDGAVRRVTELAGVPAAADLCVRAARLLKERASSRLGVDIELVK
                     RLPMGGGLGGGSSDAATTLIVLNRLWQTGLSRAQLQELAVELGADVPVFVFGRSAFAE
                     GAGERLAALQLPPAWYLVLVPETAVSTAEIFSAPELTRSTNAITIAAFSVGQGRNDLE
                     PVVCRRYPRVARHLEWLRQYGDARMTGSGACVFCGFETEAQAQRVLSELPADMRGFVA
                     RGLDRHPLWSEGN"
     gene            complement(2305..2880)
                     /gene="lolB"
                     /locus_tag="VE030_05435"
     CDS             complement(2305..2880)
                     /gene="lolB"
                     /locus_tag="VE030_05435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HZF22893.1"
                     /translation="MPLSFRLFTIGFGLAVVAGCATPGAPQALPASNAGIDRFSLTGR
                     VAVKLEQRGYSAKLNWRHAAGGDRLRLLSPVGSVIAELEADASGAMLTTGDKKVYRSG
                     DVQALTREVLGWDLPLAGLQHWVVGRADPEIPVQAEEHDARGRLTRLTQNDWRISFLA
                     YAGDSALPARMALAYDSLSLRLIIDRWDLTE"
     gene            complement(2890..4731)
                     /locus_tag="VE030_05440"
     CDS             complement(2890..4731)
                     /locus_tag="VE030_05440"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZF22894.1"
                     /translation="MLSLPSCLASLLTVACLALSACAQPGARAGLPALAADGVVASEA
                     ALDWSAQATDPSERSGPEDSFEERARRVFGTQSLSPGDLPGRDLDSDLLYKFLLAEIA
                     GQRGSYQVAAQAYLELARSTRDPRIARRATELALYGRQTEMALEAAKIWLAAEKGSTA
                     ARQTLVALFVTTNNLQAARPLLEEILASESTNVGPSLMQLHALVAKHPDKNAVLTLVS
                     DLVKPYRQLPEAHFAVAQAALAANKYDAALVEIREGLRIRPDWEAAALFQAQALTSES
                     RAKSLDYMKGFLAANPKAQEVRLNYARQLIGEKKYPEARAQFQRLLDDNPQNPDVAVT
                     VALLSVQINDLDAAEMQLKRVLELNYKDPDSVRFHLGQVNEERKRFDEAARWYLAVEG
                     GEQFVMAHARYAFMLARQNRVADARAHLQSLEAQNEAQRVQIVQAEAQLMREAKAYKE
                     SFDILKTALEAHADNPDLLYDIALAAEKLDRLDVVETSLRRLIALKPDHAQAYNALGY
                     TLADRTDRLTEARDYIEKALKLSPEDPFILDSMGWVEFRLGNHAEGLQYLQRAFDQRP
                     DPEIAAHLGEVLWVKGKKGEAEKVWRDSLRDNPGNEELQKVIKKFLP"
     gene            4976..5938
                     /locus_tag="VE030_05445"
     CDS             4976..5938
                     /locus_tag="VE030_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZF22895.1"
                     /translation="MTTTASSELTVSARALTRTYGAHRAVREVSLELRRGEVLGLLGP
                     NGAGKTTTMQMLTGNLAPSSGEVRICGIDLIDLPTQAKARIGYLPEVPPLYRELKVDE
                     YLRLAGRLHKLKKSALKPAVERAKIRCGLADMGKRLIGSLSKGYQQRVGIAQAIIHDP
                     DVIILDEPTIGLDPNQVREIRTLIRDLGRDHSVILSTHILPEVEAVCDRVQILHRGEV
                     VFYDSIAGLKQFRGGQALQIGLRNPPPVAELAGAADAEVDAINANLFRVRPRGGVDPT
                     DTLVRAAVERNWGLYLLQPALASLEEVFVHLTQREDDQSEKASA"
     gene            5935..6678
                     /locus_tag="VE030_05450"
     CDS             5935..6678
                     /locus_tag="VE030_05450"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZF22896.1"
                     /translation="MIWTIVTKELRTLFASPLAWVVLAFLQIILGWIFLTRVDYFLTI
                     QAQLSRVPNAPGLTEIVVVPMFGIASIVLLMSVPLLTMRLVAEERRNQTMAMLMSAPI
                     SMTQIVLGKFLATLAFLAIVGGLIALMGLSLLLGGKLDLGLIAANLLGLLLLTGSFAA
                     IGLFVSCLTTHPVVAAIGTLAAFLVLWLANIAASDPDSPLHLLSLLRHFENFMNGSIA
                     VTDLIYFALLTATFLILSIRRLDADRLRA"
     gene            6675..8024
                     /locus_tag="VE030_05455"
     CDS             6675..8024
                     /locus_tag="VE030_05455"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HZF22897.1"
                     /translation="MKLNRKLRLQLLLQNGLFVVLLLGLAAAIAWVTRDVKVQWDVTQ
                     SQRNTLSQASAEVLKQLTGPIAVTAYATAQDAEGDVRKTVQTFLAPYQRAKKDFMLVF
                     VDPREQPQKAQAAGVRMNGELVIEYNGRNEHLTSLTEQELTNLLVRLARSSERQVMSL
                     DGHGERKLGGRGNHDLGEFGNQLAAKGFKTAALNLAQAQDIPDNLSVLIIAGPGVDLL
                     PGEVGKIRRWVEKGGNLLWLIDSGSLRGLQALADELGLDLISGTVVDPRAGGLNLPAT
                     FALASGYGPHRITENSSITSVFPYARRIAANEGTKWHFQPLVEAAQSGWLETGGINDK
                     VAFDRNRDIRGPIVVAAALERDLGSRKQRVVIAGTGQFLSNQYVGLLGNLDLGVNMVN
                     WLAGDEALITVQPRARSDLSLELSRAGLALIGIGFLVVLPLVFLVAGGVIWWRRRQA"
     gene            8021..8878
                     /locus_tag="VE030_05460"
     CDS             8021..8878
                     /locus_tag="VE030_05460"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HZF22898.1"
                     /translation="MKSKVLVNLVLVLLLTGLVLYAFFRPKEQSDPGIRLTQLKRDDI
                     TRITVERRGSPTIQLEKRDGGWRISAPLQTRADPIQVERLADLTTATAKHKLPPGDPG
                     RYQLDPPQVRLTLNDQAFAFGRINDVTNEQYVATAGAIYLVAPFFGYGIPTEVGKLVS
                     RKLLDDAEVPIAFDFGRYRIARDERGTWTIEGVSTERQGKALSQDDFNRWADEWRFTS
                     SLGAEPRRGARGREHLVVHFRDGKKVAMEIVQKQPEFLLVRSDENMQYRFGAEVGRRL
                     LDPHVVAEK"
     gene            8888..9703
                     /gene="mutM"
                     /locus_tag="VE030_05465"
     CDS             8888..9703
                     /gene="mutM"
                     /locus_tag="VE030_05465"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003269880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HZF22899.1"
                     /translation="MPELPEVEITRRGLVPHLVGRRVETVVVRNRNLRWPIARNLGTC
                     ISGCTVRTVERRAKYLLIGCSSGWLIVHLGMSGSLRVLPHATPAGKHDHFDLVLDSGM
                     SARLTDPRRFGAVLWKAQDPLAHPLLADLAPEPLSAEFDAGWLHARTRGRSAAIKTVL
                     MDSHTVVGVGNIYASESLFRAGIHPTTAAGKISRERCDRLVRAIRETLSAAIDAGGST
                     LRDFVGSDGKPGYFQQNYYAYGRSGEPCRVCGKAIRLLRQGQRATFYCPRCQR"
     gene            9982..11721
                     /locus_tag="VE030_05470"
     CDS             9982..11721
                     /locus_tag="VE030_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZF22900.1"
                     /translation="MDSNKIRYARAALAAALFVAGSAIAADVDGNRLLNADKELEKGN
                     WLTYHGSYKSWHYSPLDQINSKNVSKLTEAWSHTASRANRGLQSFPLAIDGVLYYSSP
                     YNQVYALDGATGQILWTYKQKLNEDLVARQTHSPYNRGIAAGYGNIFIGTLDGKLAAI
                     DMKTGKLSWETKLIDSEKLTVGFTGAPLLVKDKVIIGSQGGEWPYRGPIFGVNAKTGE
                     KVWEFFTVGGNDGTKSDRRDTWGNDSWKVGGGGGWMAGAYDPETNSVWWGTANPAPLY
                     DWSGDNWMTEGARPGINLYITSVILLDPDTGNLKAYHQELPHDAWDFDSSMGEFVFLE
                     RDGKKYVVHPNKGGFIFVYDRSGKPVNVYKGVDAINFVKDIKPDGTLVGRVDYKEGKH
                     ENLCPAIAGGYSWNAGTYNPKTGLFYRVGYEWCIDLTVVKTEPITEPVVQLNIGADFT
                     FVPPKGKKSMGGHIRARDPITGKVKFEINYPGATPHASLLSTGGNVLFVPEADGTLAA
                     YDATNGKKLWTHNDGQGHNGGIISYSAKGKQYVAVMTGWGSLVGDGYGDWFGEPWKSM
                     PKDSGVLKVFALQ"
     gene            11744..12244
                     /locus_tag="VE030_05475"
     CDS             11744..12244
                     /locus_tag="VE030_05475"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZF22901.1"
                     /translation="MPASIRAGSFVPALFYRRRKLRKCVISARRPELIQRRVPNIQFG
                     KRMSALRTAHFPIRGLLAGAALAVSVAGGALAEETKTPPKPFDVKSTFRNICGFCHEN
                     YGRKAAKGPQLMNSERTDEFLFNRIKNGMPGRMAAFGSAFNDEQIHQIVIFIRNLKPG
                     EEPQNP"
     gene            12241..13041
                     /locus_tag="VE030_05480"
     CDS             12241..13041
                     /locus_tag="VE030_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZF22902.1"
                     /translation="MNRSMNMPRMRGGFALACTCAALSLPAQDADARTLAEVKSLSAI
                     SMCANRDALPYASNKPDAPGFQIEIGRAIAEGLGVPLNIEWILPRRRANVVNCDMMLD
                     SVNDPEVHEGRLRLSRPYQKSGVALGLGRDAEPIGDFKELKKGQKIGVMVSSYAGMVL
                     GKAGKSISPYAFQADLVEDVVKGELYGAAVSTAAISYYILQHPESGLRLVNAFDSEPQ
                     LTWEVSVGLRKSDQALVDAVNEVLDRMLADGSLTRIYAKYGVEHRRPQ"
     gene            complement(13061..13327)
                     /locus_tag="VE030_05485"
     CDS             complement(13061..13327)
                     /locus_tag="VE030_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HZF22903.1"
                     /translation="MALMITDECINCDVCEPECPNNAIFQGEEIYQIDPDKCTECVGH
                     YDRPQCQEVCPVDCIPLNPALPETREQLYDRYLRLTQQTVKTST"
     gene            complement(13335..13826)
                     /gene="coaD"
                     /locus_tag="VE030_05490"
     CDS             complement(13335..13826)
                     /gene="coaD"
                     /locus_tag="VE030_05490"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HZF22904.1"
                     /translation="MKTRASRAVYPGTFDPITRGHEDLVRRASTLFDEMIVAVADSRG
                     KSPFFTVEERVTMAKETLAPFDNVRVEGFSGLLMDFLHRHDAKIILRGLRAVSDFEYE
                     FQMAGMNRNLFPDVETLFLTPAEQYMFISATMVREIASLGGDVSPFVNPIVIPRLKAK
                     IRK"
     gene            complement(13823..14374)
                     /gene="rsmD"
                     /locus_tag="VE030_05495"
     CDS             complement(13823..14374)
                     /gene="rsmD"
                     /locus_tag="VE030_05495"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HZF22905.1"
                     /translation="MPARGNSVRIIGGEWRRRLIHFPGAAELRPTPDRVRETLFNWLG
                     QDLTGEICLDLFAGSGALGFEAASRGARQVVMVERDAQIFRALQATHTMLGAVQVELM
                     RADALAFLRSDARRYGVVFLDPPYHLDLLPRLLPLLPRRLNGGARVYLEADRPPDLPK
                     GYEVLRQSRAGRVHALLIKWVQS"
     gene            complement(14390..>14902)
                     /locus_tag="VE030_05500"
     CDS             complement(14390..>14902)
                     /locus_tag="VE030_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HZF22906.1"
                     /translation="GQPGIKRLDPDYFPLWLGNYVLGGGGFSSRLNEEIRQKRGFAYS
                     AYSYFNPLAQEGPFQIGLQTKKDQAAEALKVARDTLVKFVADGPTQQEFEAAKQNIVG
                     GFPLRIDSNAKILEYLSVIGFYRLPPTYLDDFPARIEAVTLAEVKDAWRRRIDPTRIA
                     TVVVGASARP"
BASE COUNT         2888 a         4737 c         4572 g         2705 t
ORIGIN      
        1 tcgatgttct gcgccggttg ctcgcctcca tagacgattc ccggcacctt gcccgttctg
       61 cgcagacggc ggctcgcacc cgttccctgc agcgagcggg acatcgcatt gacttcgatc
      121 tttgccatgt tgctctccta aaagttgacg ccatccgcga ccagatggcg ttataaaaaa
      181 ccagtgttga gtgttgagtt ttcagttttg agttgaagag cgaattccag cctggcactc
      241 aaaactcaac actcaacact caacattcag ttcgctattc catgaataac gaactgacag
      301 agtcctcgtt gctgattcgc agcatcgtct cggccagcag cgccgcgatg ctcaactggc
      361 ggatgcgttt gcagcacctg gcttcctcgc gcagcgggat ggtatcggtc accaccacct
      421 cgtccagaac ggaattctcg atgcgctcga tcgccttgcc cgataatacc gggtgagtac
      481 agtaagccag tacctttaca gcgcccgcct ctttcagcgc caacgcccct tcacacagcg
      541 tattggcggt gtccaccatg tcgtccatga tgacgcaggt gcggtccgcc acgtcgccga
      601 tgatgttcat caccgtcgcc acgttgggac gcggacgacg cttgtcgatg atggccaagt
      661 ccgattccag ccgcttggcc agcgcacggg cgcgcaccac gccgccgacg tcgggcgaga
      721 ccaccaccag atcctgatgg ccgcgcttcc agatgtcgcc cagcagaatc ggcgccgagt
      781 aaatgttgtc caccgggatg tcgaaaaacc cctggatctg gtccgcatgc agatccatcg
      841 tcagcagccg gtccacaccc gccaccacca gcatgttggc caccaccttg gcgctgatcg
      901 ccacccgcgc cgagcgcgga cgccggtcct gacgcgagta gcccaggtag ggaatcgccg
      961 tggtgatgcg cccggccgag gagcgcttca gcgcatccac catcagcatg acctccatca
     1021 ggctgtcgtt ggtcggcgtg caggtcgact gcagcacgaa tacgtccttg ccgcgcacgt
     1081 tttccagcag ctccaccatg acctcgccgt cgctgaagcg gcctacggtg gcccgcccaa
     1141 gcggaatgtt gaggtgtttg acgacgtcct cggcaagctt gggatttgcg ttgccggtaa
     1201 acaccatcaa gctgtcgtag gccattctag aaactggaaa ctggagactg gagactggaa
     1261 aaaaacaaac cctcagggcg gcaaaacaac atccaccgca aatactccgg aaactggcgc
     1321 tttccagtct ctagcctcaa gccactagtc tctagactgg ctggggaggt agggatcgaa
     1381 cctacgaatg ccggaatcaa aatccggtgc cttaccactt ggcgactccc caagaatact
     1441 agttgccctc cgaccacaga gggtgccggt ccagtccgcg cgccacgaat ccccgcatgt
     1501 ctgccggcag ctccgacagg acccgctgag cttgcgcctc ggtttcgaac ccgcagaata
     1561 cgcaggcccc ggatccggtc atgcgggcgt cgccgtactg gcgcaaccac tcgagatgac
     1621 gggccactcg cggatagcgc cggcacacca ccggctcgag atcattgcgg ccctggccga
     1681 ccgaaaaggc cgctattgtg attgcattcg tactacgtgt caattcaggc gcggagaaga
     1741 tttccgcagt ggaaacagcc gtttccggca ccagaaccag gtaccacgca ggcggcagct
     1801 ggagcgcggc gaggcgctcg ccggcccctt cggcaaaggc gctgcgaccg aacacgaaca
     1861 ccggaacgtc ggcgcccagt tccaccgcaa gctcctgaag ttgcgcgcga gagagcccgg
     1921 tttgccacaa ccggttcaac acgatgagcg tggtcgcggc atcggaactg ccgccgccca
     1981 ggccgcctcc catgggcagc ctcttgacca gctcaatatc cacgccgagg cgggagcttg
     2041 cgcgttcctt gagcagtcgt gccgcacgta cgcacaggtc ggccgcggcg ggcacgccgg
     2101 caagctcggt aactctgcgc accgcgccgt cgtctcgcac ggtgaatccc agttcgtcgc
     2161 cgtagtcgat gaagcgaaac accgtctgca acaagtgata gccgtcttca cgccgcccga
     2221 ccacgtgcag gaataagttg agcttggccg gcgcagcaaa ccgcgccgcg gcggtacccg
     2281 ccgcaccaga ctttaccgcg aggctcattc ggtcaggtcc cagcggtcga tgatgagccg
     2341 caggctgaga ctgtcatacg cgagcgccat gcgcgccggc agcgcgctgt ccccggcata
     2401 ggcaagaaag ctgatgcgcc aatcgttctg cgtgagcctg gtcagccggc cgcgcgcgtc
     2461 gtgctcctcg gcctgcaccg gaatttccgg atcggcgcgt ccaactaccc agtgctgcag
     2521 gcctgcgagc ggcaggtccc agcccaatac ctcgcgggtc agcgcctgca cgtcgccgga
     2581 gcgatacacc tttttgtctc cggtcgtcag catcgcaccg gatgcatcgg cttcgagttc
     2641 ggcgatcacc gagccgaccg gggacaacag acgcagccga tcgccgccag ccgcatgccg
     2701 ccagttcaac ttggccgagt agccgcgttg ttccagcttg accgcgacgc gaccagtgag
     2761 ggagaaccgg tcgatgccgg cattggaggc cggcagcgcc tgcggcgccc ccggggtcgc
     2821 gcaaccggcc accacggcga gcccgaaacc gatcgtgaat agacgaaaag aaagaggcac
     2881 caatgcgcct cagggcagga atttcttgat gaccttctgc agctcttcgt taccggggtt
     2941 gtcacgcagc gagtcgcgcc agaccttttc ggcctcgccc ttcttgccct tcacccacaa
     3001 tacttcgccc aggtgcgcgg cgatctcggg gtcgggacgc tggtcgaatg cgcgctgcag
     3061 gtattgcagt ccttcggcgt gattgccgag gcgaaattcc acccagccca tgctgtcgag
     3121 aatgaaaggg tcctcgggcg aaagtttgag tgccttctcg atatagtcgc gcgcctccgt
     3181 gagccgatcg gtgcggtccg ccagcgtgta gccgagcgcg ttgtaggcct gcgcgtggtc
     3241 cggcttgagc gcgatcagcc gccgcaggct agtctctacc acgtcgaggc ggtcgagttt
     3301 ctccgccgcc agggcgatgt cgtagagcag atccggattg tccgcatggg cttcgagtgc
     3361 ggtcttgagg atgtcgaacg attccttgta ggcttttgct tcgcgcatca gttgcgcctc
     3421 ggcctgcacg atctgcacgc gctgcgcctc attctgcgcc tccaggctct gcagatgggc
     3481 gcgtgcgtca gccacgcggt tctgccgcgc aagcatgaac gcgtagcgcg catgcgccat
     3541 cacgaattgc tctccgcctt cgaccgcgag gtaccatcgg gccgcctcgt cgaagcgctt
     3601 gcgctcctcg ttgacctgcc cgaggtggaa acgcacgctg tcggggtcct tgtaatttaa
     3661 ttccagcacc cgcttcaact gcatttccgc cgcgtccaga tcgttgattt gcaccgacag
     3721 cagcgctacc gtcaccgcga cgtcgggatt ctgcggattg tcgtcgagca atcgctggaa
     3781 ctgggcgcgc gcctcgggat acttcttctc gccgatcagc tggcgggcat aattcaggcg
     3841 cacttcctgc gctttcggat tggcagccag gaaacccttc atgtagtcga gtgacttggc
     3901 gcgcgactca ctggtcagag cctgcgcctg gaatagcgcc gccgcctccc agtccggcct
     3961 gatgcgcaac ccttcgcgga tttccaccag cgccgcatca tacttattgg ccgccagcgc
     4021 cgcctgcgcc accgcgaagt gtgcctcggg caactgtcgg tacggcttga ccaagtcact
     4081 caccagcgtc aggacggcgt tcttgtcggg gtgctttgcc accaatgcgt gcaactgcat
     4141 cagcgacggc ccgacgttgg tgctctcgga ggcaagaatt tcctccagca acggcctggc
     4201 tgcctgcagg ttattggtgg taacgaacag ggccaccagc gtctgccgcg ccgcggtcga
     4261 tcccttctcc gcggccagcc agatcttggc ggcttccagc gccatctcgg tctgtcgtcc
     4321 gtacaacgcc aactcggtgg cgcgccgggc aatgcgcggg tcgcgtgtgg acctggccaa
     4381 ctcgagatag gcttgcgccg caacctggta gctgccgcgc tgacccgcaa tctcggccag
     4441 cagaaacttg tagagcagat ccgaatccaa atcgcgaccg ggcaggtcgc caggagacag
     4501 cgattgcgtg ccgaagacgc gccgggcgcg ctcttcaaag ctgtcctccg gaccagatcg
     4561 ttcgctaggg tctgtggcct gcgccgacca gtccagggcc gcctcggaag ccaccactcc
     4621 gtccgcggca agcgcgggca agccagcgcg cgctcccggc tgggcacagg cgctcagcgc
     4681 caggcaggcc acggtcaaca acgatgcgag acaggagggc aatgacagca tggagtggtt
     4741 ggggccgtaa gatggaataa acgcgcgtaa tcgaatctta gaggacatgg ccgcgcgcga
     4801 agttcttcaa cccggagcac agtttaggta tatttggcgg ccgtcacaag taaccccaac
     4861 ccatccgccg acatccgttg cggcgttgcc gcccggatcc agaaggcgct gaacgcaaaa
     4921 ggggttgcaa cagcagcggc acgtagtctc cacaattttc agacaaggcg agggcatgac
     4981 caccacggca agctctgaac tcactgtttc cgcgcgggca ctgacgcgca cctacggcgc
     5041 ccatcgcgcg gtgcgcgaag tatcgctgga actcaggcgc ggcgaagtgc tgggcctgct
     5101 cgggcccaac ggcgccggca agaccaccac catgcagatg ctcaccggca atctcgcgcc
     5161 gagcagcggc gaagtgcgca tctgcggcat cgacctgatc gacctgccta cccaggcgaa
     5221 agcgcgcatc ggctatctgc ccgaagtgcc gccgctgtat cgggaactca aggtggacga
     5281 gtacctgcgc ctggcagggc gcctgcacaa actcaagaaa agcgcgctga aaccggcggt
     5341 ggagcgcgcc aagattcgct gtgggctcgc ggacatgggc aagcggctga tcggctcgct
     5401 gtcgaagggc taccagcagc gcgtcggcat cgcccaggcg atcatccacg atcccgacgt
     5461 gatcattctc gacgagccca ccatcggcct ggatcctaac caggttcgcg agatccgcac
     5521 gctgatacgc gacctcggtc gcgatcacag cgtcatcctg tccacccaca tcctgcccga
     5581 agtggaagcg gtgtgcgacc gggtgcagat cctgcaccgc ggtgaagtgg tgttctacga
     5641 cagcatcgcc ggactcaagc agttccgcgg cggccaggcc ctgcagatcg gactgcgcaa
     5701 tccaccgccg gttgcagagc tcgcgggcgc ggccgatgcc gaagtcgacg ccatcaacgc
     5761 caacctgttc cgggtacgtc cgcgcggcgg cgttgatcca accgacaccc tggtgcgggc
     5821 cgcggtcgag cgcaactggg ggctgtatct gctgcagccg gcgctggcga gcctggaaga
     5881 ggtgtttgtc catctgaccc agcgcgaaga tgatcaaagc gaaaaggcgt ccgcatgatc
     5941 tggaccatcg ttaccaagga actgagaaca ctgttcgcct cgccgctggc gtgggtggtg
     6001 ctggcgttcc tgcagatcat cctggggtgg atctttctca cccgggtgga ctacttcctg
     6061 accattcagg cgcagctatc gcgcgtgccc aatgcacccg ggctcactga gatcgtggtg
     6121 gtgccgatgt tcggtatcgc ctcgatcgtg ctgctgatgt cggtgccgct gctgaccatg
     6181 cggctggtgg ccgaagagcg ccgcaaccag accatggcca tgctcatgtc tgcgccgata
     6241 tcgatgacgc aaatcgtgct cggcaagttc ctcgccacgc tggccttcct cgccatcgtc
     6301 ggtggcctga ttgcgctgat gggactgtcg ctgctgctgg gcggcaaact ggacctgggg
     6361 ctgatcgccg ccaacctgct cggcctgctg ctgctgaccg gaagcttcgc cgcgatcggc
     6421 ctgttcgtat cctgcctgac cacccacccg gtggtggcgg ccatcggcac gctggcagcg
     6481 tttctggtgc tgtggctggc gaacatcgcc gcctccgacc cggacagccc gctacacctg
     6541 ctgtcgctgt tgaggcattt cgagaacttc atgaacggtt cgatcgcggt cacggatctg
     6601 atctacttcg cgctgctgac tgcaaccttt ctcatccttt cgatccggcg tcttgatgcc
     6661 gatcgcttgc gcgcatgaaa ctgaatcgca aactcagatt gcaactgctg ctccagaacg
     6721 gcttgttcgt ggtgctgctg ctcggactgg ccgccgccat cgcttgggtt acccgggacg
     6781 taaaggtcca gtgggatgtg acgcaaagtc aacgcaacac cctgtcgcag gcttccgccg
     6841 aggtgctcaa acagttgacc ggaccgattg ctgtcactgc ctacgccacg gcacaggatg
     6901 ccgagggcga cgtgcgcaaa accgtccaga ccttcctcgc cccgtatcag cgcgccaaga
     6961 aagacttcat gctggtattc gtcgatccgc gcgagcagcc gcagaaagcg caagcggcgg
     7021 gggtgcgcat gaacggcgag ttggtgatcg agtacaacgg cagaaacgaa cacctgacca
     7081 gcctcaccga gcaagaactc accaacctgc tggtcagatt ggcgcgctcc tcggagcgcc
     7141 aggtgatgtc cctggacggg cacggcgagc gcaagctcgg cggtcgtggc aatcacgatc
     7201 tgggcgagtt cggcaatcag cttgccgcca agggattcaa gaccgcggcg ttgaacctgg
     7261 cacaggcgca ggatattccc gacaacctga gcgtattgat cattgccgga ccgggcgtgg
     7321 acctgctgcc tggcgaagta ggtaagatca ggcgctgggt ggagaagggc ggcaatttgt
     7381 tgtggctgat cgacagcggt tcgctgcgcg gactgcaggc gctcgccgac gaactgggtc
     7441 tggacctgat ttccggcaca gtggtggacc cacgcgccgg cggtctgaac ctgccggcga
     7501 ccttcgcact ggcctcgggc tacggaccgc atcgcatcac cgagaattcc tcgattacca
     7561 gcgtgtttcc ctacgcgcgc cgtatcgcgg ccaacgaggg aacgaagtgg catttccagc
     7621 cgctggtgga agccgcgcaa agcggctggc tggagaccgg tggcatcaac gacaaagtcg
     7681 ccttcgaccg caatcgcgac atccgtggcc cgatcgtggt cgcagcggcg ctggagcgcg
     7741 accttggcag ccgtaagcaa agagtcgtaa ttgccggcac cggccagttc ctatcgaacc
     7801 agtatgtcgg cttgctcggc aacctcgatc tgggcgtcaa catggtcaac tggctggcag
     7861 gggacgaggc tctgatcacg gttcagccgc gcgcgcgttc ggatctttct ctggaattgt
     7921 cacgcgccgg cctggcgctg atcggcatcg gtttcctggt ggtgttgccg ctggttttcc
     7981 tggtcgccgg cggcgtgatc tggtggcgcc gccgccaggc atgaagtcca aagtcctcgt
     8041 gaatctggtg ctggtcctgt tgctgacggg gctggtgctg tacgccttct tccgtcccaa
     8101 ggaacaatcc gatccgggaa taagactgac acagctgaag cgcgacgaca tcacccgcat
     8161 caccgtggaa cgccgcggca gcccgaccat ccagctggaa aagcgcgacg gcggctggcg
     8221 catcagtgcg ccgctgcaaa cccgcgccga tccgatccag gtggagcggc tggccgacct
     8281 caccacggcc accgccaagc acaaattgcc gcccggtgat cccggccgct accagctcga
     8341 cccgccgcag gtcaggctca cgctcaacga tcaggccttc gccttcggcc gcatcaacga
     8401 cgtcaccaac gaacagtatg tggcgaccgc gggagcgatc tatctggtgg cacctttttt
     8461 tggctatgga attcccaccg aggtgggcaa gctggtgagc cgcaaactgc tcgacgacgc
     8521 ggaagtgccg atcgccttcg acttcggacg ctatcgcatc gccagggatg aacggggcac
     8581 gtggaccatc gaaggcgtgt ccacggagcg acagggcaag gcgctatcgc aggacgattt
     8641 caaccgctgg gccgacgagt ggcgttttac ctccagtctc ggcgccgagc cgcggcgcgg
     8701 cgcgcgcggc agggagcacc tggtggtgca cttcagggat gggaagaaag tcgctatgga
     8761 aatcgtgcag aagcaaccgg agttcctgct ggtgcgcagc gacgaaaaca tgcagtaccg
     8821 cttcggcgcc gaagtcggca gacgtctgct cgaccctcac gtagtcgccg agaagtaaac
     8881 gcgacacgtg cctgaactgc ccgaggtaga gatcacccgc cggggactgg tcccccacct
     8941 ggtcggcagg cgcgtggaga cggtggtggt gcgcaaccgc aatctgcgct ggccgattgc
     9001 gcgcaatctc ggcacgtgca tcagcggttg caccgtgcgc acggtggagc gccgcgccaa
     9061 gtatctgctg atcggctgca gcagcggctg gctgatcgtg cacttgggaa tgtcgggcag
     9121 cctgcgagtg ttgccgcacg caacgccggc cgggaagcac gatcacttcg acctggtgct
     9181 cgactccgga atgagcgcca ggcttaccga tccgcgccgc ttcggcgcgg tattgtggaa
     9241 ggcgcaggat ccgctggccc accccttgct cgccgatctc gcgccggaac cgctcagtgc
     9301 cgagttcgat gctggctggc tgcacgcccg cacccgcggc cgcagcgccg caataaaaac
     9361 ggtgctgatg gacagccata ccgtggtggg cgtcggcaac atctacgcca gcgagtcgct
     9421 gttccgcgct ggcatccatc cgacaacggc ggcgggcaaa atttctcgtg aacgctgcga
     9481 ccgtctggtg cgggcaatac gggagacgct cagcgcagcc atcgacgccg gcggcagcac
     9541 gctgcgcgac tttgtcggct ccgatggcaa acccggttat ttccagcaaa attactacgc
     9601 ctatggcaga agcggcgagc cctgccgcgt gtgcggaaag gctatccggc tgctgcgtca
     9661 gggtcagcgc gcgacctttt attgcccgcg gtgccagcgc taacgccatc cgctgcccat
     9721 tgattcaact agtgttcgtt agcaatggct cactttccct tgactagcca ggtaaacatc
     9781 ccgagaatcg cgcggtaccg aagtgcgaaa ccgggccctc gaacccgggt tcgaggctgt
     9841 tgtcgcgcct ccagcagttt ggcactagga gtggatcagg tacggcaagt cgcctgcctt
     9901 ccggtgttaa tccaaagaac gacccaacgg cgacattgca gtttcttggg gaagagctaa
     9961 aacttttaga ggaaaatcat tatggacagc aataagattc gctacgcgcg cgctgcgctc
    10021 gcggcggccc tgtttgtagc gggcagcgca attgccgcag atgtggacgg caatcgactg
    10081 ctcaatgcag acaaggagct cgaaaaaggc aactggctaa cgtatcacgg ctcctacaag
    10141 tcctggcatt acagtccgct cgaccagatc aacagcaaaa atgtcagcaa gcttacggaa
    10201 gcctggtcgc acacggcttc gcgcgccaat cgcggcctgc aaagcttccc gctggccatc
    10261 gacggcgtgc tgtattactc cagcccgtac aaccaggttt atgcactgga cggcgccacc
    10321 ggtcagatcc tgtggaccta caagcagaag ctgaacgaag acctggtggc gcggcagacc
    10381 cactcgccgt ataaccgcgg tattgccgcc ggctacggca atatcttcat cggcaccctg
    10441 gacggcaagc tggccgcgat cgacatgaag accggcaagc tcagctggga aaccaagctg
    10501 atcgactccg agaaactcac ggtcggattc accggcgccc cgttgctggt caaggacaag
    10561 gtgatcatcg gttcgcaagg cggcgagtgg ccctaccgcg gcccgatctt cggcgtcaac
    10621 gccaagaccg gcgagaaggt ctgggaattc ttcaccgtcg gtggtaacga tggaaccaag
    10681 agcgaccggc gcgacacctg gggcaatgac tcctggaagg tcggcggcgg cggcggctgg
    10741 atggccggtg cctacgatcc cgaaaccaat tcggtgtggt ggggtaccgc caatccggct
    10801 cctctgtacg actggtcggg cgataattgg atgaccgaag gtgcgcgtcc cggcatcaac
    10861 ctgtacatca cgtcggtcat cctgctggat ccggataccg gcaacctgaa ggcatatcac
    10921 caggaactgc cgcacgacgc ctgggacttc gactcgtcga tgggcgaatt cgtgtttctg
    10981 gagcgcgacg gcaagaagta tgtcgtgcac cccaacaaag gcggtttcat tttcgtctat
    11041 gaccgttccg gcaagcccgt caatgtgtac aagggcgtcg atgccatcaa tttcgtcaaa
    11101 gacatcaaac ccgacggcac attggtcggg cgcgtcgact acaaggaagg caagcacgag
    11161 aacctctgcc cggcaattgc gggagggtat agctggaacg cggggaccta caatccgaag
    11221 accggcctgt tctaccgggt cggctacgag tggtgtatcg acctcaccgt tgtaaagacc
    11281 gagccgatta cggaaccggt ggtacagttg aacatcggcg cggacttcac gttcgttccg
    11341 cccaagggca aaaaatcgat gggcggccac atccgtgcgc gcgatccgat caccggcaag
    11401 gtcaagttcg aaatcaacta ccccggtgca accccccacg ccagcctgct gagcaccggt
    11461 ggcaacgtgc tcttcgtgcc cgaggcggac ggcacgttgg ccgcgtatga cgcgaccaat
    11521 ggcaagaagc tgtggacgca caacgacggc cagggccaca acggcggcat catcagctat
    11581 tcggccaagg gcaagcagta cgtcgcggtg atgaccggct ggggttcgct ggtcggcgac
    11641 ggctacggcg actggtttgg cgagccgtgg aagagcatgc cgaaggactc cggagtgctg
    11701 aaggtgtttg cgctgcaata accgggaata caaccggcta taattgcctg catcaatcag
    11761 ggcggggagt ttcgtccccg ccctttttta tcgtcgtcga aaactgcgca agtgcgttat
    11821 tagcgcgcgc cgtccggaat tgattcagcg ccgggtacct aacattcaat ttggtaaaag
    11881 gatgtctgct ttgagaacag ctcatttccc gatcaggggt ctgctggcgg gcgccgcctt
    11941 ggccgtcagc gtggccggcg gggctttagc cgaagagacc aagactccgc ccaaaccctt
    12001 tgacgtgaag agcacgtttc gaaacatctg cggcttctgc cacgaaaact acggccgcaa
    12061 ggccgccaag ggaccgcaac tgatgaactc ggagcgtacc gacgagttcc tgttcaaccg
    12121 catcaaaaac ggcatgccgg gccgcatggc ggctttcggc agcgccttca acgacgagca
    12181 gatccaccag atcgtgatat tcatccgcaa tctcaaaccc ggcgaagaac cccagaaccc
    12241 atgaaccgat cgatgaacat gccccggatg cgcggcgggt ttgcgttggc ctgcacgtgt
    12301 gccgcgttgt cgttgcccgc ccaggacgcc gatgcgcgca cgcttgccga agtgaagtcg
    12361 ctgagcgcca tttccatgtg cgccaatcgg gacgcgctcc cctacgccag caacaaaccg
    12421 gatgcgcccg gattccagat cgaaatcgga cgtgccatcg ccgaaggcct cggggttccg
    12481 ctcaacatag aatggatcct gccgcgccgg cgcgccaacg tcgtcaattg cgacatgatg
    12541 ctcgacagcg tcaatgaccc ggaagtccac gaaggcagac tgcgcctgtc gcgtccttac
    12601 cagaaatcgg gggttgcgct cggtctcggc cgggatgctg agcccatcgg cgacttcaag
    12661 gaacttaaga agggccagaa gatcggcgtg atggtcagtt cgtatgccgg catggtgctc
    12721 ggcaaggctg gcaaaagcat ctcgccgtac gctttccagg ccgacctggt ggaagatgtg
    12781 gtgaagggcg agttgtacgg ggctgcggta tcaacagcgg ccatcagcta ctacattctc
    12841 cagcacccgg aatccgggtt gcggctggtg aatgccttcg acagcgaacc tcagctcacc
    12901 tgggaagtct cggtgggatt gcgaaaatcg gaccaggcac tcgtggatgc cgtcaacgag
    12961 gtgctcgaca gaatgctggc cgacggcagc ctcacccgca tctatgccaa gtacggcgtc
    13021 gagcatcgcc ggccgcagta ggccccggcc ggtgcgcggc tcaggtggaa gtcttgactg
    13081 tctgctgggt caggcgcagg tacctgtcgt agagctgctc cctcgtttcc ggcaatgccg
    13141 ggttcagcgg gatgcaatcc acggggcaga cttcctggca ttgcgggcgg tcgtagtggc
    13201 cgacgcactc ggtgcatttg tccggatcga tctggtagat ctcttcgccc tggaaaatgg
    13261 cgttgttcgg gcactccggc tcgcacacgt cgcagttgat gcattcgtcg gtgatcatca
    13321 gggccataag gaactcactt ccgaattttc gccttcaacc gcgggatcac gatcggattg
    13381 acgaacggag atacgtctcc gcccaggctg gcaatctcgc gcaccatggt cgcggagatg
    13441 aacatgtatt gttcggccgg cgtcaggaac agggtttcga catcgggaaa caggttgcgg
    13501 ttcattccgg ccatctgaaa ctcgtactcg aaatcggaca cggcacgcag tccccgcagg
    13561 atgatcttgg cgtcgtgcct gtgcaggaag tccatcagca gtcctgaaaa gccttccacc
    13621 ctcacgttgt cgaaaggcgc gagcgtttcc ttggccatcg tcacgcgctc ttcgacggtg
    13681 aaaaacgggc tcttcccccg gctgtcggcc accgcgacga tcatctcgtc gaacaaagtc
    13741 gaagcgcggc gcaccaggtc ctcgtggccg cgcgtgatcg ggtcgaaggt gccgggatag
    13801 accgccctgc tggctcgcgt cttcatgatt ggacccattt tatcaggaga gcgtgcacgc
    13861 gcccggcgcg ggattggcgc agcacctcgt agccctttgg caaatccggc gggcggtcgg
    13921 cttcgaggta gacgcgcgcg ccgccgttaa gccggcgagg cagcagcggc aacaggcgcg
    13981 gcagcagatc cagatggtag ggcggatcca gaaacaccac gccgtaccgg cgcgcatcgg
    14041 atcgcagaaa cgccagcgca tcggcacgca tcagttcaac ctgcacggct ccaagcattg
    14101 tatgagtcgc ttgcagcgcg cggaatatct gcgcgtcccg ttccaccatc accacttgcc
    14161 tggcgccgcg cgaggcggcc tcaaacccga gggcgccgct gccggcaaac agatccagac
    14221 agatctcccc ggtgaggtcc tgtcccagcc agttgaacag ggtttcgcgc acccgatccg
    14281 gagtaggtcg cagctccgcc gcgccgggaa agtggatgag gcgacggcgc cactcgccgc
    14341 cgatgatgcg aacgctgttg ccacgcgccg gcatcgcgcc ttcaccggtt caaggccttg
    14401 ccgacgcgcc gacgaccacg gtggcgatgc gggtggggtc gatacgccgg cgccaggcat
    14461 ctttcacttc cgccaaggtc accgcctcga tccgagcggg aaagtcatcc agataagtcg
    14521 gcggcaggcg atagaagccg atcaccgaca agtactcgag gatttttgcg ttgctgtcga
    14581 tgcgcagggg aaatccgccg acgatgttct gtttcgccgc ctcgaattcc tgctgcgttg
    14641 gaccatcggc gacgaatttc accagcgtgt cgcgcgccac cttgagcgcc tccgcggcct
    14701 gatccttttt ggtctgcaac ccgatctgga acggcccttc ctgcgcgagc ggattaaagt
    14761 aactgtaggc gctgtaggca aagccgcgct tctggcggat ttcctcattg agccgggagg
    14821 agaacccgcc accaccgagc acgtagttgc ccagccaaag cggaaagtaa tcaggatcga
    14881 ggcgcttgat tcccggctgg cc
//
LOCUS       DASQOP010000135         2906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_54784, whole genome shotgun sequence.
ACCESSION   DASQOP010000135 DASQOP010000000
VERSION     DASQOP010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54784"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..168)
                     /locus_tag="VE030_05505"
     CDS             complement(<1..168)
                     /locus_tag="VE030_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011656385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZF22907.1"
                     /translation="MISFSILDLSPIPRGATASEALRNTLALAQRADKLGYKRYWLAE
                     HHNMPGIASAAT"
     gene            complement(200..706)
                     /locus_tag="VE030_05510"
     CDS             complement(200..706)
                     /locus_tag="VE030_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HZF22908.1"
                     /translation="MSLSMFKASVPVFTRQLANLSAILHKAEAHAAARKIDPAVFINA
                     RLAPDMFPLSRQVQSASDSAKGCAARLAGVEIPSYADTETTFPELQARIGKTVAFLQS
                     LGPELIDGSEERAIVLKLRGQETKFSGQYYLLNFALPNFFFHAVTAYDILRHNGLEIG
                     KQDFLGSF"
     gene            827..1375
                     /locus_tag="VE030_05515"
     CDS             827..1375
                     /locus_tag="VE030_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZF22909.1"
                     /translation="MQRKSFGKMQCPIARGLERVGEWWSILILRDALHGLTRFDQFEQ
                     SLGIAPNMLARRLAGLVKAGLLERRRYSERPPRYEYVLTERGRDFQPVIVAMHAWGNR
                     HFAPEGQSVLLVNARTGEPADPVIVDRNSKHPLADTEFRVAPGPAAAERTRRKLASSA
                     WGIALHPPTIDKRGARKRRNRA"
     gene            1372..2808
                     /locus_tag="VE030_05520"
     CDS             1372..2808
                     /locus_tag="VE030_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZF22910.1"
                     /translation="MNSSIAATADASPPPAIRSREGMNFRTRLLLEGRIVPTLFLLAW
                     PNILVMLAQASTGLVETWFVSRLGTDALAGMALVFPGFMMMQMLSAGAVGGGISSAIA
                     RALGGGHRDDADALVLHAIIINLALGVLFSVLVLVFGRVLYASMGGEDASLEAALSYS
                     NVVFAGAWLVWLMNALASVLRGTGNMLVPSVAICAGFVLLVPLSPVLIFGFGPIPAMG
                     IAGGGIAVVVTVALSTAVMAWYLWSGRSAVHPKFAPLRKVLFVDILRVGAVGSVSTLQ
                     TTLVFVLTTALVGAAVGPGAVAGYGTAARLEYLLIPLVFGLGAPLVALVGTNIGAGQQ
                     ARALRIALTGGAIAFVITEAIGLVAAIWPEAWLGLFGDDMRMIETGSAYLRVVGPAYG
                     FFGLGLALYFASQGAGRLFWPLLSGFFRLIIATGGGWILLRLTGSLNWLFAALSLALV
                     VYGVSLVAAIRSGAWFANDRVHIPQGHK"
BASE COUNT          449 a          928 c          957 g          572 t
ORIGIN      
        1 ggtcgcggcg ctggcgatcc ccggcatgtt gtgatgctcc gcgagccagt agcgcttgta
       61 ccccagcttg tcggcgcgct gcgccagcgc aagcgtgttg cgcaatgcct cggacgcggt
      121 cgcgcccctg gggatcggtg agaggtcgag tatggaaaaa gagatcatgc gtaacgattc
      181 ctggtgtgcc ggctgtcact cagaagctgc cgaggaaatc ctgcttgccg atctccaggc
      241 cattatggcg gaggatgtcg taggcggtga cggcatgaaa gaagaaatta ggcagggcga
      301 aattcagaag ataatactgg ccggaaaatt tcgtttcctg gccacggagc ttgaggacga
      361 tcgcgcgttc ctcgctgccg tcgatcaact cggggccaag gctttgcagg aacgccacgg
      421 tcttgccgat gcgtgcctgc agttcgggaa aggtggtctc ggtatccgca tagctgggta
      481 tctcgacgcc tgcgagacgg gccgcgcagc ctttcgccga atcgctggcc gactgcacct
      541 ggcgcgagag cgggaacatg tcgggcgcga gcctcgcatt gatgaagacc gccggatcga
      601 ttttccgtgc cgcagcgtgc gcctccgcct tgtgcaggat cgcggaaagg ttcgccagtt
      661 ggcgggtgaa cacggggacg gaagccttga acatggatag ggacatggca gttctcctgg
      721 atgataaagc ggcattgtgc gcgacttcgc cgctgcgcgc cgcaccggac tccgccagcg
      781 gccaaagtaa cttgcattat aatagttact tggctatcct gccgccatgc agcgcaaaag
      841 cttcggaaaa atgcaatgcc cgatcgcccg cggcctggaa cgggtcggcg agtggtggag
      901 cattctcatc ctccgcgacg ccctgcacgg actgacgcgc ttcgaccagt tcgagcaaag
      961 cctgggcatt gcgcccaaca tgctggcgcg gcgtcttgcc ggactggtca aggccggtct
     1021 tctcgaacgg cgccgttaca gcgagcgccc gccgcgctac gagtatgtgc tgacggagcg
     1081 aggccgggat ttccagcctg tcatcgtcgc catgcatgcc tggggcaaca ggcatttcgc
     1141 gcccgaaggc cagagcgtgc tgctggtcaa tgcgcgaacc ggcgagcccg cggatcctgt
     1201 gatcgtcgat cgcaacagca agcacccgct tgccgacacc gagttcaggg tggcacccgg
     1261 accggccgcg gccgaacgca cccggcgcaa gcttgcctcc agcgcctggg gcattgcgct
     1321 gcacccgccg accatcgaca agcgcggcgc gcgcaagcgg cgaaaccggg catgaattcc
     1381 tccatcgccg ccacggcgga cgcgagcccg ccgcccgcca tccgttctcg cgaagggatg
     1441 aatttccgca cccgcctgct gctggaaggg cggatcgtgc ccacactatt cttgttggcg
     1501 tggcccaaca tcctggtgat gctggcgcag gcctccacgg gattggtcga gacctggttc
     1561 gtctcgcgcc tcggcaccga tgcgctggct ggcatggcgc tggtgttccc cggcttcatg
     1621 atgatgcaga tgctctctgc gggtgctgtc ggcggcggaa tttcctcggc aatcgcgcgc
     1681 gcactgggcg gcgggcatcg cgacgacgcc gatgcgctgg tgctgcacgc catcatcatc
     1741 aatcttgcgc tcggtgtgtt gttctccgtg ctggtacttg tcttcggccg cgtcctctac
     1801 gcttccatgg gcggagaaga cgcatccctg gaagcggcgc tcagctactc gaacgtggtc
     1861 ttcgccggcg cctggctagt ctggctgatg aacgcgctgg caagcgtact gcgcggtacc
     1921 ggcaacatgc tggtgccttc ggtggcgatc tgcgccggat tcgtcctgct ggtgccgctt
     1981 tcgcccgtgc tgatattcgg attcgggccg atccccgcga tgggcatcgc cggcggcggc
     2041 atcgcggtcg tggtcaccgt cgcgctcagt actgcagtca tggcctggta cctctggtcc
     2101 ggccgcagcg ccgtgcatcc gaaattcgcg ccgctgcgca aagtcctgtt cgtcgacatc
     2161 ctgcgcgtcg gtgcagtggg ttcggtcagc acgctgcaaa cgacgctggt cttcgtgttg
     2221 accacggcgc tggtcggcgc cgccgtcggg ccgggagccg tcgccgggta tggcaccgct
     2281 gcgcgcctgg aatatctgct cattccgctc gtattcggcc tgggtgctcc gctggtcgcg
     2341 ctggtcggta ccaacattgg cgcgggccag caggcacgcg cactgcgcat tgcgttgacg
     2401 ggtggcgcga tcgccttcgt catcaccgaa gcaatcggcc ttgtcgcggc gatctggcct
     2461 gaagcgtggt tggggctgtt cggcgacgac atgcggatga tcgaaaccgg cagcgcctat
     2521 ctgcgcgtcg tcggccccgc gtacggattc ttcggcctgg gcctggcgct ttattttgcc
     2581 tcgcagggcg cgggccgcct gttctggcct ttgctcagcg gcttcttccg cctcatcatt
     2641 gcgaccggcg gcggctggat cctgctgcgc ctcaccgggt cgttgaactg gctgtttgcg
     2701 gcgctttccc tggcactcgt cgtctacggg gtctcgctcg tcgcggcgat ccgttccggc
     2761 gcgtggttcg ccaacgaccg ggtgcacatt cctcaagggc acaaatgacc gaacccgcca
     2821 agactgtccg cgtttccaac tggccgctgg tgatcgccgc ccccctcttc ccaggcctct
     2881 cccgccaggg gagaggggga attgaa
//
LOCUS       DASQOP010000136         7294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1667226, whole genome shotgun sequence.
ACCESSION   DASQOP010000136 DASQOP010000000
VERSION     DASQOP010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7294
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1667226"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(278..3748)
                     /locus_tag="VE030_05525"
     CDS             complement(278..3748)
                     /locus_tag="VE030_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HZF22911.1"
                     /translation="MALAQVSLDDKYTLQKGRVFLTGTQALVRMPMMQHLRDKAAGTN
                     TACYISGYRGSPLGGYDQQLWAAKKQLAGHSIVFQPGVNEDLAATACWGTQQAHFEGD
                     NKYDGVFAIWYGKGPGVDRTGDAFRHGNLAGSASLGGVLCLMGDDHTCESSTTAHQSE
                     FAMVDAMIPILNPAGVQEVLDYGLYGWALSRYSGCWVGLKCMKDTIDASASVDVSPAR
                     VRIVVPTDFQMPAGGLNIRWPDTPLEQEARLHTWKLEAAKAFARTNKLDQIVVDSPDA
                     KLGIATCGKSYMDVRQALAYLGIDDAEAARLGLRIYKVGMTWPLEPAGAVKFAEGLQK
                     IIVVEEKRALIETQLKETLYGSAAAPEVIGKRDELGKILFQSNAALDPNEVAIAIGRR
                     ILERVNDAQLAARVAEQEQLAARVPEKPAMDRTPYFCSGCPHNTGTRVPEGSRALAGI
                     GCHFMAQWMDRNTAGFTQMGGEGASWIGEAPFVKTPHVFQNIGDGTYYHSGLLAVRAA
                     AASGVNVTYKILYNDAVAMTGGQHVDGPLTVPQITLQVYSEGANKIAVVTDEPEKYPS
                     TVEWAPGVTIHHRDDYDQVQRQFRQVPGLSVIIYDQTCAAEKRRRRKRNQYPDPAKRA
                     FINDLVCEGCGDCGVKSNCVSVLPLETEFGRKRMIDQSSCNKDYSCVNGFCPSFVTVH
                     GGGVRKGKSAPAGQNVDTLFAAVPEPRLPSLDQPYGIMVTGVGGTGVVTIGAIVGMAA
                     HLEGKGFGGLDMAGLAQKGGSVWSHLQIAADPSDIKTVRLGSGSARLVLGCDLVVSAS
                     QKTLDTARTGTRVVANTHQQMTGEFTRNANMQFPAQSLKRTITKGVGEGNAEFVDATR
                     IATALIGDSIATNMFMLGYAFQKGLVPVSSAAINKAIELNGAAMKMNQTAFLWGRRAA
                     ADLAAVERLVAPGPETAATTTISQTLEEMIARRVEFLSGYQNAAYADKYRSLVERVKQ
                     AETTKAKGLSGLAETVARYYFKLLAYKDEYEVARLYADPAFMEKIKSQFEGDYKLHFH
                     LAPPLFSKRDPVTGELRKSEYGTWVFSAFKVLAKLRPLRGTALDIFGYTPERRMERRL
                     IGEYEAVIEEIIAKLGPDNHALAMKIASIPEEIRGYGHVKERNLKAAKLMEADLLLAL
                     RSPEKVRSAA"
     gene            complement(3867..4328)
                     /gene="ssb"
                     /locus_tag="VE030_05530"
     CDS             complement(3867..4328)
                     /gene="ssb"
                     /locus_tag="VE030_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HZF22912.1"
                     /translation="MASVNKVILIGNLGRDPEVRYMPSGDAVANISIATTETWKDKNG
                     EKQEKTEWHRVAMFGKTAEIAGEYLKKGSQVYIEGRLETRKWTDKEGQERYTTEIRAD
                     RMQMLGSRSGGSERMAPPEDDAPKAAAVPAKKSGGAAKGSSLEDLEDDIPF"
     gene            complement(4331..5683)
                     /locus_tag="VE030_05535"
     CDS             complement(4331..5683)
                     /locus_tag="VE030_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZF22913.1"
                     /translation="MTPQELRASVGLAGIFGLRMLGMFIILPVFALYAERLPGGHDHT
                     LVGLALGAYGLTQACLQVPFGWLSDRWGRKRTIYLGLVIFALGSFLAALAQDITVVIL
                     GRVIQGAGAISAAVIALTADLTRDDQRTKAMAIIGMTIGATFALSMVAGPVLDHMMGV
                     PGIFALTGFLALAAIVVVRFVIPDPSQSRPGRTPEGAAAPVGRVLLDQQLLRLNYGIF
                     ALHAVLMALFVVVPFSLRAQLPAGSHWMVYLPVMCGAIVLMLPPMLAAERKGLQKPVF
                     VGAVGVLIVALLLLALGSNGLAGLAAGLLMFFAAFNLLEATLPSLISKLAPAGSKGTA
                     IGVYSSVQFIGTFTGAAVGGWLSQHYGAEAVFGFCAALSVVWLLLTLGMRVPKSLDTR
                     RYQLPKMDSQAVPGLTRRLAGLPGVREVMLSGEGVAYLKVDSQGFDEQNVIKLLGGEI
                     "
     gene            5786..>7294
                     /locus_tag="VE030_05540"
     CDS             5786..>7294
                     /locus_tag="VE030_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010090680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZF22914.1"
                     /translation="MDYIRVRGARTHNLKNIDLDLPRNRLIVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLELMEKPDVDLIEGLSPAIAIEQKATSHNPRSTVGTVT
                     EIHDYLRLLYARVGEPHCPDHGIALSAQSVSQMVDHVLQLPQDTRLMILAPLLVGRKG
                     ENLELFEDLRAQGFVRLRVDGEVHDIDSVPKLAKTRKHTIEIVVDRLKIRPEAKQRLA
                     ESFETALRHADGRALAVEMDSGKEHLFSARFACPICSYSLPELEPRLFSFNNPMGACP
                     RCDGLGSITFFDPKRVVAFPHLSLAAGAIKGWDRRNQFYFQMLASLGEQYGFDVDTPF
                     EQLPKNVQDLVLYGSGRDKVRFLYVGERGNKYQREHPFEGIVPNLERRYRETDSIVVR
                     EELAKFLASQPCPECKGTRLRREARYVFVAGKPIYEVSAMPLKRALQFFQEIELEGAR
                     LAIAEKIVREIANRLQFLVNVGLDYLTLDRSADTLSGGEAQRIRLASQIGSGL"
BASE COUNT         1318 a         2420 c         2241 g         1315 t
ORIGIN      
        1 aacagcccgg ggccggcgcg cagcagcgcg gcctggatgg tgtcgccggc atatccggga
       61 tcggcgggtt tcagctttgg ccttttcgca gtgattgcgt gcacggagcc aagcctaatc
      121 ctgaattgtt acaggaagat gatcaccacc tctctccccg ccccctacgc cgcacggagg
      181 gagcatcaac aaaaaaaaca ggcgaccgcg tgatcgcccg cttccgccct ttcaccctct
      241 ccgcctttca agggggagag ggcggggaga ggtggtgtta cgccgccgag cgtacctttt
      301 cgggcgaacg caacgccagc aacagatccg cttccatcag cttggccgcc ttgagattgc
      361 gttccttgac gtggccgtag ccgcggattt cctcgggaat ggaggcgatc ttcatggcca
      421 gcgcatggtt gtccggtccc agcttggcga tgatttcctc gatcacggct tcgtattcgc
      481 cgatcagccg cctctccatc ctgcgctcgg gggtgtagcc gaaaatgtcc agcgcggtgc
      541 cgcgcagtgg gcgcagcttc gccagcacct tgaacgcgga gaacacccag gtgccgtact
      601 cggacttgcg caactcgccg gtcaccggat cgcgtttcga gaacagcggc ggcgccagat
      661 ggaagtgcag cttgtagtcg ccttcgaact gcgacttgat cttttccatg aacgccggat
      721 cggcatacag gcgcgcgact tcgtactcgt ccttgtaggc cagcagcttg aagtaatagc
      781 gcgccaccgt ctcggcgagc ccgctaagac ctttcgcttt ggtggtctcg gcctgcttca
      841 cgcgctcgac cagggaacga tatttatcag catatgcagc gttctggtaa ccggacagga
      901 attcgactcg ccgcgcgatc atttcctcga gcgtctgcga aatggtcgtg gtcgcagccg
      961 tttccggccc cggcgccacc agtcgctcga ccgcggccag gtccgccgca gcccgccgtc
     1021 cccacaggaa agcggtctgg ttcattttca tcgcggcacc gttcaattcg atcgccttgt
     1081 tgatcgcggc gctcgagacc ggcaccaggc ccttctggaa tgcgtatccg agcatgaaca
     1141 tgttggtggc gatcgagtcg ccgatcagcg cggtggcgat ccgcgtcgca tccacgaatt
     1201 ccgcgttgcc ttcgccgacg cccttggtga tggtgcgctt gagcgactgc gccgggaact
     1261 gcatgttggc gttgcgggtg aattcgccgg tcatctgctg gtgagtgttg gccaccacgc
     1321 gcgtgccggt acgcgcggta tccagggtct tctggctggc ggacaccacc aggtcgcaac
     1381 cgagcaccag cctggcgctc ccggagccga ggcgcacggt cttgatgtcg gacggatcgg
     1441 ccgcaatctg caggtgactc cacaccgagc cgcccttctg ggcgaggccg gccatgtcca
     1501 gcccgccgaa acccttgcct tcgaggtgcg ccgccatgcc gacgattgcg ccgatggtca
     1561 ccaccccggt gccgccgacg ccggtcacca tgattccgta aggctgatcc agcgacggca
     1621 gacgcggttc aggaacggcg gcgaacaacg tgtcgacgtt ctgcccggcg ggggcgctct
     1681 ttcccttgcg cacgccgccg ccgtggacgg tgacgaagct cgggcagaag ccgttgacgc
     1741 aggaataatc cttgttgcat gacgactggt cgatcatgcg cttgcggccg aattcggtct
     1801 caagcggcag caccgacacg cagttggatt tcactccgca gtcgccgcag ccttcgcaca
     1861 ccagatcgtt gatgaacgcg cgcttggccg gatccgggta ctggttgcgt ttgcgccggc
     1921 ggcgcttttc cgccgcgcaa gtctgatcgt aaatgatcac cgacaggccc gggacctggc
     1981 ggaactggcg ttgcacctgg tcatagtcgt cgcgatgatg gatcgtgacg cccggcgccc
     2041 actcgacggt cgaggggtac ttctccggct cgtcggtgac gaccgcgatc ttgttcgcgc
     2101 cttcggagta cacctgcagc gtgatctgcg gcaccgtcag cggaccatcg acgtgctggc
     2161 cgccggtcat ggcaacggca tcgttgtaga ggatcttgta ggtgacattg acgcccgacg
     2221 cggcggccgc gcgcaccgcc agcagccccg agtggtagta ggtgccgtcg ccgatgttct
     2281 ggaatacatg cggcgtctta acgaatggcg cctcgccgat ccagctcgcc ccttcgccgc
     2341 ccatctgggt gaacccggcg gtgttgcggt ccatccactg cgccatgaag tggcagccga
     2401 ttccggcgag cgcacgcgaa ccctcgggca cgcgggtgcc ggtattgtgc ggacagcccg
     2461 aacaaaagta cggcgtgcga tccatcgccg gtttctccgg cacgcgcgcg gccagttgct
     2521 cctgctccgc cacgcgcgcc gccagttgcg catcgttcac ccgctcgaga atgcggcgtc
     2581 cgatggcaat cgcgacctcg ttgggatcga gcgcggcgtt ggactggaac aggatcttgc
     2641 cgagctcatc gcgcttgccg atcacttccg gcgcggcggc gctgccgtac agtgtttcct
     2701 tcaactgggt ctcgatcagc gcgcgctttt cttcgacgac gatgatcttc tgcagccctt
     2761 ccgcgaactt caccgcgccg gcgggctcga gcggccaggt catgccgacc ttatagatgc
     2821 gcaggcccag gcgcgcggcc tccgcgtcgt cgatgccgag ataggcaagc gcctgccgca
     2881 catccatata ggacttgccg caggtggcga tgccaagttt cgcatccggt gaatccacca
     2941 ctatctggtc cagcttgttg gtccgcgcaa acgccttggc ggcctcaagc ttccacgtat
     3001 gcaaacgtgc ttcctgctcc agcggcgtgt ccggccagcg gatgttcaac ccgcccgcgg
     3061 gcatctggaa atcggtcggg accacgatgc gcacgcgcgc cgggctgacg tcgaccgagg
     3121 cactggcgtc gatcgtatct ttcatgcact tcagccccac ccagcaaccg gagtagcgcg
     3181 acagcgccca gccgtacaga ccgtagtcga gcacttcctg cactccggca ggattcagga
     3241 tcgggatcat cgcatccacc attgcgaatt cgctctggtg cgcggtggtg gaggattcgc
     3301 aggtgtggtc atcgcccatc aggcacagca cgccgcccaa cgatgcggat ccggccagat
     3361 tgccatggcg gaaggcgtcg ccggtgcggt ccacgcccgg acccttgccg taccagatcg
     3421 cgaacacgcc gtcgtacttg ttgtcgccct cgaaatgcgc ctgctgcgta ccccagcaag
     3481 cagtggcggc aaggtcctcg ttcacgcccg gctgaaacac gatgctgtgc ccggccaact
     3541 gcttcttggc cgcccacagc tgctggtcgt agccgcccag cgggctgccg cgatagccgg
     3601 agatatagca ggctgtgttg gtacccgcgg ccttatcgcg caggtgctgc atcatcggca
     3661 tgcgcaccaa cgcctgcgtg ccggtcagaa aaacgcggcc tttctgaagc gtgtatttgt
     3721 cgtcgagcga aacttgcgcc agtgccatga ctaacctcta agaaacgttc ggaagcagcg
     3781 gatgcacgtc aaccggcgcg cgccccgccg cggtaggaca gaaaacatcg gtattggtgc
     3841 cggtcggcgc cgaaaatccc cggacatcaa aagggaatat cgtcctcgag atcctccagg
     3901 ctgctccctt tggctgcacc accggatttc ttcgccggta cggccgccgc cttgggagcg
     3961 tcgtcttccg gcggcgccat gcgttcggac ccgccggagc gcgaacccag catttgcatg
     4021 cgatcggcgc gaatttccgt ggtgtagcgc tcctggcctt ccttgtcggt ccacttgcgc
     4081 gtctccagcc tgccctcgat gtaaacctgg gagcccttct tcaggtattc gccggcgatc
     4141 tcggcggtct tgccgaacat cgccacgcgg tgccattcgg tcttctcctg cttctcgccg
     4201 ttcttgtcct tccacgtctc ggtggtggca atgctgatgt tggccacggc gtcgccgctg
     4261 ggcatgtagc gcacttcggg gtcgcgcccg aggttgccga tcaggatcac tttgtttacc
     4321 gaagccatat tcagatctcc ccgcctaaaa gtttgatgac attttgttcg tcgaagccct
     4381 ggctgtccac cttgagatac gctacgccct cgcccgagag catcacttcg cgcacgcccg
     4441 gcaggcccgc gagtcgccgc gtcaatcccg gcaccgcctg cgaatccatc ttcggcagct
     4501 ggtaacggcg cgtgtcaagg cttttcggca cgcgcatacc cagcgtcaac aatagccaga
     4561 ccaccgacag cgcggcgcag aacccgaaca ctgcctccgc gccgtaatgc tgcgacagcc
     4621 agccgcccac tgccgcaccc gtgaacgtac cgatgaactg cacgctgctg tacacaccga
     4681 tcgccgtgcc cttgctgccg gccggcgcaa gcttcgaaat cagcgagggc agcgtggcct
     4741 cgagcagatt gaacgcggcg aagaacatca gcaatccggc ggccagtccc gccagaccgt
     4801 tgctgccgag cgcaagcaga agcagcgcca cgatcagcac gccgaccgca ccgacgaaaa
     4861 ccggcttctg caatcccttg cgttccgccg ccagcatcgg cggcagcatc agcacgatag
     4921 cgccgcacat gaccggcaga taaaccatcc agtggctgcc tgcgggcagc tgcgcgcgca
     4981 acgaaaacgg cacgaccacg aacagcgcca tcagcaccgc gtgcagcgcg aatatgccgt
     5041 agttgagccg cagcaactgc tgatccagca gcaccctgcc gactggcgcc gccgctccct
     5101 caggggttct gccgggacgg ctctgcgacg gatcgggaat gacgaagcgc accaccacga
     5161 tcgccgcaag tgccaggaaa ccggtcagcg cgaaaatgcc tggcacgccc atcatatgat
     5221 ccagcaccgg accggccacc attgaaagtg cgaacgtggc gcctatggtc atgccgatga
     5281 tggccatcgc tttggtgcgc tgatcgtcgc gggtaagatc ggcggtgagc gcgatcaccg
     5341 ctgccgaaat cgcacccgcg ccctgtatca cccggcccag gatcaccacg gtgatgtcct
     5401 gcgctagagc ggccaggaag ctgccgagcg cgaagatcac cagccccaga tagatggtgc
     5461 gcttgcgccc ccagcggtcg gagagccagc cgaacggcac ctgcaggcag gcctgggtca
     5521 ggccataggc cccgagcgcg agcccgacca gcgtgtggtc atggccgccg ggaaggcgct
     5581 ccgcgtacag cgcaaaaacc ggaagaatga tgaacatccc cagcatgcgc aacccgaaaa
     5641 tgccggccag gccgacactt gcgcgcagtt cctggggcgt catccggtcc gggaagagca
     5701 ttcggcgaaa gggggtaaac cgcggattgg gaaaagaaat tgaagtgcag tatattaaca
     5761 ggttgactca ttcaccttcc agcgaatgga ctacatccgc gtccgcggcg cacgcaccca
     5821 caacctcaag aacatcgatc tcgacctgcc gcgcaaccgg ctgatcgtga tcaccgggct
     5881 gtccgggtcc ggcaaatcct cgctcgcatt cgacaccctc tacgccgagg gccagcgccg
     5941 ttacgtcgag tcgctgtccg cctacgcgcg gcaattcctg gagttgatgg aaaagccgga
     6001 cgtggatctg atcgaaggct tgtcgccggc gattgccatc gagcagaagg ccaccagcca
     6061 caacccgcgt tccaccgtag gcaccgtcac cgagatccac gattacctgc ggctgctgta
     6121 tgcgcgcgtc ggcgagccgc actgcccgga ccatggcata gcgctgtcgg cgcagagcgt
     6181 ctcgcagatg gtggatcacg tactgcaact accgcaagac acgcgactga tgatcctggc
     6241 gccgctgctg gtgggccgca agggcgaaaa cctggaactc ttcgaggacc tgcgtgcgca
     6301 gggcttcgtg cgactgcgcg tggacggcga ggtgcacgac atcgactccg tgcccaagct
     6361 cgccaagacc cgcaagcaca cgatagaaat cgtggtggac cgcctgaaga tccggccaga
     6421 agcgaagcag cggctggcgg aatcgttcga gacggcgctg cgccacgccg acgggcgcgc
     6481 gctggcggtg gagatggaca gcggcaagga gcatctgttt tcggcgcgct tcgcctgccc
     6541 gatttgcagc tattccttgc ccgagctcga gccgcgcctg ttctcgttca acaacccgat
     6601 gggggcgtgt ccgcgctgcg acgggctggg gagcatcacc ttcttcgatc cgaaaagggt
     6661 ggtggccttt ccgcacctgt cgctcgccgc cggcgccatc aagggctggg accggcgcaa
     6721 ccagttctat tttcagatgc tcgccagcct cggcgaacaa tacgggttcg acgtcgacac
     6781 gccgtttgag cagctgccga agaacgtcca ggacctggtg ctgtacgggt cggggcggga
     6841 caaggtgcgc ttcctctacg tcggcgagcg cggcaacaag taccagcgcg aacatccgtt
     6901 cgaaggcatc gtaccgaacc tggaacggcg ctatcgcgaa acggattcga tcgtggtacg
     6961 cgaggaactg gcgaagtttc ttgccagcca gccctgcccc gagtgcaagg gcacgcgact
     7021 gcgccgcgaa gcgcgttacg tcttcgtggc cggcaagccg atctacgaag tgagtgccat
     7081 gccgctgaaa cgtgcgcttc agttcttcca ggagatcgaa ctcgagggcg ccaggctggc
     7141 gatcgccgaa aagatcgtgc gcgaaatcgc caatcggctt cagttcctgg tcaacgtcgg
     7201 cctcgactac ctcacgctcg accgctcggc agacacgctc tccggcggtg aagcgcagcg
     7261 catcaggctg gcctcgcaga tcggttccgg cctc
//
LOCUS       DASQOP010000137         5465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2271030, whole genome shotgun sequence.
ACCESSION   DASQOP010000137 DASQOP010000000
VERSION     DASQOP010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5465
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2271030"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="VE030_05545"
     CDS             complement(<1..958)
                     /locus_tag="VE030_05545"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZF22915.1"
                     /translation="MSSRLRRIFTLSASGGEGRGEVGERSLVFVLAAIQFTHVMDFMI
                     MMPLGPQLMRVMLISPQQFGLLVSAYTLTAAVAALAVAFYTDRFDRRKTLVFLYAGFV
                     ISTLLCGIAPGYGALLAARAVAGAFGGVAGATVHSIIGDAIPEQRRGAATGMIMSAFA
                     LSSIVGVPIGLLLAAHFSWRAPFLLLVVVSTLVLILTWKILPPMRGHIVEGQSHRPLE
                     QMRAVFGTANHLRAFVFMFALMFAGFSVIPFISPYMVANVGLKETDLPYLYFFGGLAT
                     VFTSRYIGKLADRHGKRQIFTLIGLISIAPLLITTNLPPVPVP"
     gene            complement(1048..1722)
                     /locus_tag="VE030_05550"
     CDS             complement(1048..1722)
                     /locus_tag="VE030_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22916.1"
                     /translation="MFRRCLIALALVAAFVPIARAAAEPVPIEQEPRHRLKFENAHVR
                     FFDVQLEPGYQSLYHWHHNDGVFVNIASSPTIAQDWGAEPTTRGERAIGEAYFIAYAA
                     KPKAHRVANPGPGMYRVIDAEIMSSCGPMNAARESPNQTLVVDNERVFVTRLILHPGE
                     STELYAPCGMLVSVSGGNVVFDTPDGKEKLAMDRGEFKWRKLQQTVKLTNVGTNVFHG
                     VDIRIN"
     gene            complement(1850..2029)
                     /locus_tag="VE030_05555"
     CDS             complement(1850..2029)
                     /locus_tag="VE030_05555"
                     /inference="COORDINATES: protein motif:HMM:NF023369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HZF22917.1"
                     /translation="MKGYESDVTRFIREFLAKHPEVVAKQEQARATWWDRPQDFKENA
                     ALEAAEVPRKSYEYY"
     gene            2141..4186
                     /locus_tag="VE030_05560"
     CDS             2141..4186
                     /locus_tag="VE030_05560"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZF22918.1"
                     /translation="MRRSPIRVPPLAKRVPHVTTVHGERRVDDYHWLRNKASPSVRAY
                     LRAENAYTTAMMKPSEGLQKKLYKEMLARIKETDTDVPYREGAYYYYSRTEKGKQYRI
                     FCRRQGSPDAPEAVILDVNRLAQGHKFYSVGAVEISPDGNVLAYSSDTSGFREYTMQL
                     KDLRNGRVLSDRIEKVRSAAWASDNRTLFYVREDGTKRAYRLYRHLVGSKDDQLIYEE
                     ADARFSLSVARSRSRAYLFLTSHSATTSEVRYLRADAPAFPWQLVAARQPEHEYYVDH
                     GQGLFYIVTNDKGRNFRLVTAPAEDPSPGNWKEKIPHRAEVMLEGVDVFARHLVAHER
                     AGGFPRLRVMRLADGESHEIAMPEPVYSVGGGANFEFDTDVFRFHYDSYITPDTEYDY
                     HLDRRERKLLKQREVRGYEPAKYRSELHYSVAPDGTRVPISLVYRIDRRADAAQPLLL
                     YGYGAYGFPLDVDFSSARLSLLDRGVIYGVAHVRGGGEMGKRWHDEGRMLKKMNTFTD
                     FISCAEHLIGEHYTDPGKLVIQGGSAGGLLVGAVTNMRPDLFRAVVADVPFVDVINTM
                     LDDRLPLTTGEYEEWGNPKDKVYYDYMKSYSPYDNLAAKDYPAMLAETSLNDSQVMYW
                     EPAKYVARLRRLKTDGNPLLFRINMDAGHGGASGRYEFLKEIAFTYSFIIGVLGLIK"
     gene            4209..4502
                     /locus_tag="VE030_05565"
     CDS             4209..4502
                     /locus_tag="VE030_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22919.1"
                     /translation="MTDPLRPTSQTGEQLAGQIRLAEKFLAKYPRHAGGEEISFVLAI
                     HYVQAACAAERETDKAAFAHKARAMAGEFAKTYPESLRAAALPHVLEGLPAGR"
     gene            complement(4510..4920)
                     /locus_tag="VE030_05570"
     CDS             complement(4510..4920)
                     /locus_tag="VE030_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22920.1"
                     /translation="MNPGNTASLLPRGATLRSAGRRVAAAAAVALCALFACPALAEQA
                     MVIWKNRECGYFILQMKSGYGIFRWIDGPQPNDGDVLEGDFRSAGERLVDNKTADVPT
                     TILLYAFALNRSAIAARMPAKCKALPDYIPFKGR"
     gene            complement(4917..5288)
                     /locus_tag="VE030_05575"
     CDS             complement(4917..5288)
                     /locus_tag="VE030_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22921.1"
                     /translation="MLRKTIRALALCLLCAGPLGAQTEIPQDIQKGELIWRDPSCFFF
                     VLKVDEHYALFEFLGGPSPMVGHIFEGKLTGFGSRRIENTTEGKPTMVYSEAYDLSKS
                     QMEKKIPKFCKKRKEFEALEG"
BASE COUNT         1076 a         1688 c         1725 g          976 t
ORIGIN      
        1 cgggcaccgg caccggcggc aaattggtgg taatcagcag cggcgcgatc gaaatcaggc
       61 cgataagtgt gaatatctgc cgcttgccgt ggcggtccgc cagcttgcct atgtagcggg
      121 acgtgaacac ggtggcgagt ccgccgaaaa agtacagata gggcaggtcg gtctccttca
      181 atccgacatt ggctaccata taggggctga tgaacgggat caccgaaaac cccgcgaaca
      241 tcaatgcaaa catgaatacg aaggcgcgca ggtgattggc ggttccgaac accgctctca
      301 tctgctccag aggccgatgc gactggcctt cgacgatatg gccgcgcatg ggaggaagaa
      361 ttttccaggt gagaatcaga accagcgtcg agacgacgac gaggagcagg aacggcgcgc
      421 gccaggagaa atgggcggca agtaacaatc cgattggaac gccgacgatg gaagacagcg
      481 cgaacgcgga catgatcatg ccggtcgccg cgcctcgacg ttgttcggga atcgcgtcgc
      541 cgatgatgga gtgtacggtc gcaccggcca cgccgccgaa ggcgccggcg accgcgcgcg
      601 cggccagcag cgcgccatag cccggcgcga ttccgcacag cagagtcgat atcacgaagc
      661 ccgcgtagag aaagaccagc gttttgcggc gatcgaagcg gtcggtgtag aaggccacgg
      721 ccagcgctgc gaccgctgcc gtaagggtgt aggccgaaac caggagcccg aactgctggg
      781 gcgagatgag catgacgcgc atcaattgcg ggccgagcgg catcatgatc atgaagtcca
      841 tgacgtgggt gaactggatg gcggccagca cgaagacgag cgaccgttcg cccacctcac
      901 cccggccctc tcccccggag gcggagaggg tgaaaatgcg ccgcaatcgt gacgacatgc
      961 ttcgtatccc ggttattgcc ttaagcccgc gcagcgcacc ttctccgctc cggggagaag
     1021 gcgggggatg aggtggttgg ggtgtggcta gttgatgcgg atgtctaccc cgtggaacac
     1081 gttggttccg acgttggtga gtttcacggt ctgctgcagc ttgcgccatt tgaattcgcc
     1141 acggtccatg gcgagcttct ccttgccgtc aggagtgtcg aacacgacgt tgccgccgga
     1201 taccgaaacc agcatgccgc aaggtgcgta gagttcggtg gattcgccgg gatgcaggat
     1261 caaccgggtg acgaacacgc gttcgttgtc gacgaccagc gtctgattcg gactttcgcg
     1321 cgccgcattc atcgggccgc aggagctcat gatctcggca tcgataacgc ggtacatacc
     1381 cggaccagga ttcgcaaccc ggtgcgcctt cggtttggcc gcgtaagcga tgaaataagc
     1441 ctcgccgatg gcacgctcgc cgcgcgtcgt cggctcggca ccccagtcct gggcgatggt
     1501 cggcgaagaa gcgatgttga cgaatacgcc gtcgttgtgg tgccagtgat agagcgactg
     1561 gtatccgggt tcgagctgca cgtcgaaaaa gcgcacgtgc gcgttctcga acttcagacg
     1621 atggcgcggc tcctgctcga tcggcaccgg ctctgcggcg gcgcgcgcaa ttgggacgaa
     1681 cgcggcgacg agcgcaagcg caatcaggca gcgacgaaac atttttaccc tccccctctc
     1741 cccaccctct ccggctaggg gagagggagc ttgaaaatca atcctggccc gcgcagcgca
     1801 cgttctccgc cccggggaga aggcggggga tgaggtggtt gggtgggtct caatagtact
     1861 cgtaggattt cctgggaacc tcggcggctt ccagcgccgc attttccttg aagtcctgcg
     1921 gccggtccca ccaggtggcg cgggcttgtt cctgtttcgc gaccacctcg gggtgcttgg
     1981 caagaaattc ccggatgaag cgggtgacgt ccgactcgta gcccttcata ctgctttccg
     2041 attcgttgat cgagccgcaa ttgtaaggtc tgccgggccc gggtcctatg gatttcagcc
     2101 tgcaggtgtg attaggccgt gtcatcccag gagcgttcaa gtgcgtcgaa gccccattcg
     2161 cgtgccaccg ctagccaagc gcgtacccca cgtgacgacc gtgcacgggg agcgccgcgt
     2221 cgacgactac cactggctgc gcaacaaggc cagcccttct gtacgcgcct acctgcgcgc
     2281 cgagaacgcg tacaccacgg cgatgatgaa gccctcggaa ggcctgcaga agaagttgta
     2341 caaggagatg ctggcgcgca tcaaggaaac cgacaccgac gtcccgtacc gcgagggtgc
     2401 ctattactat tactcccgca ccgaaaaagg caagcagtac cgcattttct gccgcaggca
     2461 aggcagcccg gacgctcccg aggccgtgat cctggacgtg aaccgcctgg cgcagggtca
     2521 caagttctac tccgtgggag cggtcgaaat cagccccgac ggcaacgtgc tcgcctattc
     2581 gtcggatacc agcggcttcc gcgaatacac gatgcaactg aaggacctgc gcaatggcag
     2641 ggtgctctcc gaccgcatcg agaaagtgcg ctcggcggcg tgggcgagcg ataaccgcac
     2701 tctgttctac gtcagggagg acggcaccaa gcgcgcctac cgcctctatc gccacctggt
     2761 agggagcaaa gacgaccagc ttatttacga ggaggccgat gcgcgtttct cgctttcggt
     2821 cgcgcgctcg cgcagccgcg cttacctgtt cctgacttcc cacagcgcca ccacttccga
     2881 agtgcgctat ctgcgggccg atgcgcctgc gttcccctgg caactggtgg cggcgcgcca
     2941 gccggagcac gaatactacg tggaccatgg acaggggctg ttctatatcg tcaccaacga
     3001 caaggggcgc aacttccgcc tggtcacggc gcccgcggaa gatccctctc ccgggaactg
     3061 gaaagaaaag atcccgcacc gcgcggaagt gatgctggag ggcgtggacg tattcgcccg
     3121 gcatctggtc gcgcacgaac gcgccggcgg ctttcccagg ctgcgcgtca tgcgcctcgc
     3181 ggacggcgaa tcccacgaaa tcgccatgcc cgagccggtg tactcggtgg gcggcggagc
     3241 caatttcgag ttcgataccg acgtattccg ctttcactac gattcgtaca tcactcccga
     3301 tacggagtac gactatcacc tcgaccgccg cgaacgcaaa ttgctcaagc aacgcgaagt
     3361 tcgcggttac gagccggcga agtatcgctc ggagttgcac tactcggtcg ccccggacgg
     3421 cacgcgcgtg ccgatttcgc tggtgtatcg catcgaccgt cgcgccgacg ccgcacagcc
     3481 gctgctgctc tacgggtacg gcgcctacgg ctttccgctg gacgtggatt tcagttcggc
     3541 gcggctgtcg ctgctcgatc gcggcgtaat ctatggcgtg gcgcacgtgc gcggcggcgg
     3601 cgagatgggc aagcgctggc acgacgaggg gcggatgctc aagaaaatga acacgtttac
     3661 cgatttcatt tcctgcgcgg agcatctgat cggcgagcac tacaccgatc cgggcaagct
     3721 ggtgatccag ggaggcagcg ccggggggct gctggtgggc gcagtaacca acatgcggcc
     3781 cgatcttttc cgcgcggtcg tcgccgacgt gccgttcgtg gacgtcatca acaccatgct
     3841 cgacgaccgg ctgccgctta ccaccggcga atacgaggag tggggcaatc cgaaggacaa
     3901 ggtttactac gattacatga agtcgtattc gccctacgac aacctggccg cgaaagacta
     3961 tccggcgatg ctggccgaga cttcgctcaa cgacagccag gtgatgtact gggaacccgc
     4021 caagtacgtg gccaggctgc ggcgcctgaa gaccgacggc aatccgctgc tgtttcggat
     4081 caatatggac gccggacacg ggggcgcctc ggggcgatac gagttcctca aggagatcgc
     4141 gtttacctat tcgttcatca tcggcgtgct cggcctcata aaatagcgcc ccgtggccgc
     4201 cgcgcacgtt gacagacccg ctgcggccga cctcacagac gggggagcag cttgccgggc
     4261 agatcaggct cgccgagaag tttctcgcga agtaccctcg gcatgccggc ggcgaagaga
     4321 tatccttcgt cctcgcaatt cactacgtgc aggcggcgtg cgccgcggaa cgggaaacag
     4381 acaaggccgc gtttgcgcac aaggcgcggg ccatggccgg cgagttcgcg aagacctatc
     4441 cggaaagcct gcgcgccgcg gcgctgcctc acgtgctcga agggctgccg gccggcaggt
     4501 gagggggtgt tagcggcctt tgaagggaat gtagtccggc aatgccttgc acttcgccgg
     4561 catgcgggcg gcgatcgcgc tgcgattcag cgcaaacgca tacagcagga tcgtggtcgg
     4621 cacatcggcg gtcttgttgt cgaccaggcg ctccccggcg gacctgaaat cgccttccag
     4681 cacgtcgccg tcattcggct gcggtccgtc tatccaccgg aaaatgccgt agcccgactt
     4741 catctgcagg atgaagtacc cacattcgcg gttcttccag atcaccatgg cttgttccgc
     4801 aagcgcgggg catgcgaaca gggcgcagag agcaactgcg gcggccgcgg ccactcgcct
     4861 cccggcactg cgcaacgtcg cgccgcgcgg cagcaagctg gccgtattgc cggggttcat
     4921 ccctccagag cctcgaactc cttgcgcttc ttgcagaact tcggaatctt tttctccatc
     4981 tgcgacttcg acaggtcgta cgcctccgag tacaccatgg tcggcttgcc ttcggtcgta
     5041 ttctcgatgc gtcgcgaacc gaatccggtg agcttgcctt cgaagatatg gcccaccatc
     5101 gggctcggtc cgcccagaaa ttcgaacagc gcgtaatgct cgtccacctt gagtacgaag
     5161 aaaaagcagc tcggatcgcg ccagatcagt tcgcctttct gaatgtcttg cgggatttcg
     5221 gtctgtgccc ccagcgggcc ggcacagagc aggcacagcg ccagcgcccg aatagttttg
     5281 cgtaacattg gaaatccttg cgagatatga tgattgaccc gaaccgcgcg cgggctcacc
     5341 ggagcgccgg gcgggaactc cgagccaatg atacccccgc aggctttggc acgggcaaac
     5401 aaaggcatat ccgctggtgt aacattctgc ttcacacaag aaaccgcccg cgtgatcatg
     5461 ccggg
//
LOCUS       DASQOP010000138         5844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1064350, whole genome shotgun sequence.
ACCESSION   DASQOP010000138 DASQOP010000000
VERSION     DASQOP010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5844
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064350"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VE030_05580"
     CDS             <1..457
                     /locus_tag="VE030_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZF22922.1"
                     /translation="KLFQNNDAGVAPLLKHESGVLALSDAGAHLIFMCDAGFGLHFLR
                     HWVRETGTFTLEEGVRRLTSDPAAKYRIPDRGRIAPGAWADLLLFDPAKVGQSALVRR
                     KDLPAGGARMLRHPEGVHGVWVNSIQIHDGRDYLPLESGPGQVLTKFLQ"
     gene            454..1275
                     /locus_tag="VE030_05585"
     CDS             454..1275
                     /locus_tag="VE030_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HZF22923.1"
                     /translation="MITVRRVAGALGAEVDGVDLSKPLSAGLVAELRQAWLENLVLFF
                     RDQPLTPGQYLSFAQNFGTPADYPLLKGIDGFPKIIEVKKLEHERINFGGIWHSDTAY
                     LDTPPMASMLLARETPPHGGDTLFANMYLAWDALSEGMRRMLRGLKAVNTSAKADASK
                     TREDRIREEGKPAAEFTAEHPVARTHPETGRTVLYVNAGHTVRFAGMTEEESRPLLDY
                     LFRHQVRPEFTCRFAWRPGSIALWDNRCTQHNPVNDYHGYRRVMHRITLAGDIPR"
     gene            complement(1335..1565)
                     /locus_tag="VE030_05590"
     CDS             complement(1335..1565)
                     /locus_tag="VE030_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22924.1"
                     /translation="MRGERHRVRASGCDYEPVGRIAVKRGRQRPVALSGLSSRFDRKN
                     HAHKARRLAPIAALAVILLGILMPVLYAKFDA"
     gene            1740..2597
                     /locus_tag="VE030_05595"
     CDS             1740..2597
                     /locus_tag="VE030_05595"
                     /inference="COORDINATES: protein motif:HMM:NF015147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HZF22925.1"
                     /translation="MRTLASHRLAAVALIGASFLSGCQTIEGILESSAKPTARIIGTE
                     MKNLSLERIDLVFNVEVANPYTVDLPLLDLAYNVGSGGTSLLQGTIKPSGAVQARGTS
                     VIQLPARITFASLVRTLKGVKPGSVVPYQADLTLGVDAPVVGRLTLPLSKSGDLPVPA
                     APEVELASFEIAKLGMDETIATAKLQVKNTNPFVLNLSKLSIELALGANKVANTSLAN
                     SAKIAPGQSVAVDVPLSFSPRSFGLAVFNLLRGNKAAYSLVGSLEAGTPYGPLLLPFN
                     RNGNTIIKK"
     gene            2610..4064
                     /locus_tag="VE030_05600"
     CDS             2610..4064
                     /locus_tag="VE030_05600"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF004816.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZF22926.1"
                     /translation="MTDEVAFASAKKLASLIRRRKIGCVELLDLYLKRVARLNPKINA
                     VVVLDDKRARERARKADRAAAKKDWWGPLHGVPMTVKESFDVEGLPTTWGRADMKGNI
                     ATRHALSVHRLMGAGAVIFGKTNVPVMLADWQTFNPVYGTTGNPWDVTRIPGGSSGGA
                     GAALAAGLTGLELGSDIGASIRNPAHYCGVFGHKPTFELCPIHGHNLPPNLSTRDMMV
                     IGPLARSADDLEIVLGIIGQPDEMASRGVKIALAKPKPSVKKLKIAVLANAENAEVDQ
                     SVQAAVLAAAKCFADSGATVSDTARPDIDLLEAHKTFVHLVRGATSTALTDEQFKSQQ
                     ELAAKFAHGDDSYPAWMMRANTMSHRDWHGWNEKRELMRLKWAEFFREWDLLLCPAAA
                     TAAFPHNQKGERWERMVVVNGKPQPSTTQMFWAGYPGLAWLPSSVAPAGFTEQRLPVG
                     VQIVGPQYGDLATIAAAKFLEQEFQPFVAPPGFV"
     gene            complement(4287..4553)
                     /locus_tag="VE030_05605"
     CDS             complement(4287..4553)
                     /locus_tag="VE030_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22927.1"
                     /translation="MKFSKLIVTAGFAIAASGAFAETGVSIADPGYVANVYGRASIPA
                     VKTAGPLVIRPADVNVAGREPVHGTTSMVVSTRKVDVNEVFGRT"
     gene            complement(4764..5228)
                     /locus_tag="VE030_05610"
     CDS             complement(4764..5228)
                     /locus_tag="VE030_05610"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HZF22928.1"
                     /translation="MIKKIVGNFFLVLAVATGAANAADWEVLNAQPEEAMLLDENSIH
                     HDGSVWKAWAVQSYKRTEYLGEPVYPHRSRVTLYEVDCKIGQLGYAAWSFQSGELGGG
                     TTVWADTARGVMYFRPDADSSESALLERVCGAALAHDGDRLVSDSRPNAAQE"
     gene            complement(5458..5817)
                     /locus_tag="VE030_05615"
     CDS             complement(5458..5817)
                     /locus_tag="VE030_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22929.1"
                     /translation="MTKKRLLEEAIIKITKQAGVETSGTFHLTSVSRDHGGLFIMEVM
                     GPERKRLCEEVKRAVAGFPSDTESGCIVLLPDVVDEIIFAAQSEPAVFLPASSRTVTG
                     KALLDVHRTGDENVPQV"
BASE COUNT         1140 a         1855 c         1725 g         1124 t
ORIGIN      
        1 caaactgttc cagaacaacg atgccggcgt ggcgccactg ctcaaacacg aatccggcgt
       61 gctggcactc tcggacgctg gcgcgcatct catcttcatg tgcgacgcgg gattcggcct
      121 gcacttcctg cgccactggg tgcgcgagac cggaacgttc acgctcgaag aaggcgtgcg
      181 ccgattgacc tccgatccgg cggcgaaata ccgcatcccg gatcgcggcc gcatcgcacc
      241 gggcgcgtgg gcggatctgt tgttgttcga tccggctaaa gtgggacagt ccgcgctagt
      301 acgccggaag gacctgcccg cgggcggcgc acgcatgctc cgccatccgg aaggcgttca
      361 cggcgtctgg gtgaacagca tacagattca cgatgggcgg gattacctgc cgctcgaatc
      421 cggtcccgga caggtgctca cgaagttcct gcaatgatca cagtcaggcg ggtggccggc
      481 gcgctcggcg cagaagtcga cggcgtggat ctctcgaaac cgctttctgc cggattggtg
      541 gcggaacttc gccaggcgtg gctggaaaac ctggtgctgt tcttccgcga tcagccgctc
      601 acccccgggc agtatctgtc gttcgcgcaa aatttcggaa cgccagccga ctacccgctg
      661 ctcaagggca tcgacggatt cccgaagatc atcgaagtca agaaactgga gcacgagcgc
      721 atcaatttcg gcggcatctg gcactcggac acagcctatc tcgacacgcc gccgatggcc
      781 tcgatgctgc tcgcgcggga aacgccgccc cacggcggcg acacgttgtt cgccaacatg
      841 tacctggcat gggatgcgtt atccgaaggc atgcgccgca tgctgcgtgg actcaaggca
      901 gtcaacacat cggcaaaagc ggatgcctcg aaaacccgcg aagaccgcat tcgggaagag
      961 ggcaagccgg cggcggaatt caccgcggaa catccggtgg cgcgcacgca ccccgagacc
     1021 ggtcgcaccg tgctctacgt gaatgccggc cacacggtgc ggttcgcggg catgaccgaa
     1081 gaggagagcc ggccgctgct cgattacctg ttccgtcacc aggtgcgccc ggaattcacc
     1141 tgtcgtttcg cctggcggcc gggatcgatc gcgttatggg acaaccgctg cacacagcac
     1201 aatccggtga acgactacca tggctaccgg cgcgtcatgc accggatcac gctggccgga
     1261 gacattccgc gctgaggcgc ggaatcgggc gaacccttcc cacccgactg ttcgtcaacg
     1321 acgcagcgag gtgctcaggc gtcgaatttt gcgtaaagaa ccggcatgag gatgccgaga
     1381 aggatcaccg ccagcgccgc gatcggcgcg agccgccgtg ccttgtgcgc gtggttttta
     1441 cggtcgaaac gagagctcag gccagataat gcgactggtc gctgccggcc tcgtttcacg
     1501 gcgatccggc cgacaggctc atagtcgcaa ccgctcgcgc gcacgcgatg ccgctcgcca
     1561 cgcatgacgc tgcaattcgc cgctcgcggg ccgcgaaaat ctggaagcca tgaactcctc
     1621 aaggtaagag ccgaaaggcg gaacggtgcc ttcgggtatt gtctttcctc ttttcctctc
     1681 gcttctcgcc tctcaccgtt ttccgttagc attgccggac tttcatatcc aaacagccca
     1741 tgcgcaccct cgccagtcat cgccttgccg ctgttgccct gatcggcgcc tctttcctca
     1801 gcggttgcca gaccatcgaa ggcatccttg aatccagcgc caagccgacg gcacgcatta
     1861 tcggcaccga aatgaagaat ctgtctctgg agaggatcga cctagtcttc aacgtggagg
     1921 tcgccaatcc ctatacggtg gacttgccgc tgctggacct cgcttacaac gtgggcagcg
     1981 gaggcaccag ccttctgcag ggaaccatca agccttccgg cgccgtgcag gcacggggca
     2041 ccagcgtaat ccagcttcct gccaggatca cctttgcttc gctggtcagg accctcaaag
     2101 gtgtgaagcc gggatcggtc gtgccctacc aggccgatct gaccctgggc gtcgatgcgc
     2161 cggtggtggg aaggctgacg ctgccgctat cgaaatccgg cgatctgccg gttccggccg
     2221 cgccggaggt cgagcttgcc tctttcgaga tcgccaaact cgggatggac gaaaccattg
     2281 ccaccgcaaa gttgcaagtg aagaacacca acccatttgt gctcaatctg tccaagctca
     2341 gcatcgaact cgcgctcggc gctaacaaag tcgccaatac cagtctcgcg aattcggcga
     2401 agattgcgcc cggccagtcc gttgccgtgg acgtgccgct gtcgttctcg ccgcgctcct
     2461 ttggtctcgc ggttttcaat ttgctgcgcg gaaacaaggc cgcttacagt ctggtcggct
     2521 cgctcgaagc gggcacgccg tatggtccgc tgttgctgcc gttcaaccgc aacggcaaca
     2581 cgataatcaa aaaataagga ggctccgaaa tgacggatga agtcgcattc gcgtccgcga
     2641 aaaaactcgc ttcgctgatc cgccgcagga aaatcggctg cgtcgagctg ctcgatctgt
     2701 atctgaaacg agtcgcgcgc ctgaatccta agatcaacgc ggtggtcgtg ctcgacgaca
     2761 agcgcgcccg cgagcgggca cgcaaagccg accgcgctgc cgcaaagaag gactggtggg
     2821 gtccgctgca cggcgtgccg atgacggtca aggaatcttt cgacgtcgag gggttgccca
     2881 ccacctgggg ccgcgccgac atgaagggca acatcgccac gcgccatgcg ctttccgtcc
     2941 atcgactgat gggtgccggc gccgtcatat tcggcaagac caatgtgccg gtaatgctcg
     3001 ccgactggca aaccttcaat ccggtttacg gcaccaccgg caacccctgg gacgtcacgc
     3061 gtattccggg cggctcttcc ggtggcgccg gcgccgccct cgccgccggg ctgaccggac
     3121 tcgaactggg cagcgacatc ggcgcctcga tccgcaatcc ggcgcattac tgcggcgtgt
     3181 tcgggcataa accgactttc gagctgtgcc cgattcacgg acacaacctg ccgccgaacc
     3241 tgtccacgag agacatgatg gtgatcggcc cactggctcg cagcgccgac gatctcgaaa
     3301 tcgtgctcgg gatcatcggc cagccggacg agatggcgtc gcgcggcgtg aagatcgcgc
     3361 ttgccaagcc caagccttca gtgaagaaac tgaagatcgc agtgctggcc aacgccgaga
     3421 atgcggaagt ggatcagtcg gtgcaggcgg ccgtgctggc ggcggccaaa tgcttcgcgg
     3481 acagcggtgc aaccgtcagc gacacggcgc gcccggacat cgacctgctg gaagcccaca
     3541 agactttcgt ccatctggtg cggggcgcga cttcgacggc gctcaccgac gaacagttca
     3601 agagccagca ggagctggcg gcgaagtttg cgcacggcga cgacagctac ccggcgtgga
     3661 tgatgcgcgc caataccatg tcgcatcgcg actggcacgg ctggaacgag aagcgggagc
     3721 tgatgcgcct caagtgggcg gagttcttcc gcgaatggga cctgctgctc tgcccagccg
     3781 ccgcgaccgc agctttcccg cacaaccaga aaggcgaacg ctgggagcgg atggtcgtgg
     3841 tcaacggcaa gccccagcct tccacgaccc aaatgttctg ggccggatac ccgggactgg
     3901 cctggttgcc ttcgagcgtc gcgcccgccg gattcaccga acagcgcctg ccggtcgggg
     3961 tgcagatcgt cggtccgcag tacggcgacc tcgccaccat cgccgccgca aagtttctgg
     4021 aacaggagtt ccagcctttc gtggcaccgc cgggattcgt gtgaggaagt gccgggatga
     4081 gtgatgagtg atgggtgatg ggtgatgggt gatgggtgat gggtaaagtc taatcaatcc
     4141 tgttttccac ttcgggtttc ctttgcgtag ctttgcggcc tttgcgttaa cgcttttgtg
     4201 ccggccggca ctttgacacg cgttcccgaa agaaaaacgc cgcacggatg aataccgtgc
     4261 ggcgttcgtt tgtcggcact ccgggtttat gtgcggccga agacctcgtt gacgtcgact
     4321 ttcctcgtgg acaccaccat cgaggtcgtg ccatgcacgg gctcgcggcc ggcgacgttc
     4381 acatccgcgg gacggataac cagcgggccg gcggtcttca ctgccggaat gctggcgcgg
     4441 ccataaacgt tcgctacgta gccgggatcc gcaatgctca cgcccgtttc ggcaaacgcg
     4501 ccgctggcgg caatggcaaa tccggcagta acaatcagtt ttgaaaactt catctataac
     4561 tcctttcgat gcttacaacg ataacaagga tgaaaaaccg gcacttcatc gtgtgaagcg
     4621 accggcgcta ccgcgggcgg aaagacgatc ttcccgtcct tggtccggtc agtcaggcga
     4681 tgccgaatgt gtctctccgt ggaacactac cgtcatctca cgggcaatac cttccaaatt
     4741 cgggaaacac gtcggcgcct gtcctattcc tgagccgcgt tgggccgcga gtcgctcacc
     4801 aggcgatcgc cgtcgtgtgc cagcgcggcc ccgcagactc gctcaagtaa tgcagattcc
     4861 gaagagtcgg cgtcaggccg gaagtacatc acgcctcgtg ccgtgtcagc ccacacggtg
     4921 gttcctccac caagctcgcc cgattgaaag ctccaggccg cgtagccgag ttgccctatc
     4981 ttgcaatcga cctcgtacag cgtgaccctc gaccggtgcg gatacaccgg ctcgcccagg
     5041 tattcggtcc tcttgtagct ctgcactgcc caggctttcc aaacggatcc gtcgtgatga
     5101 atgctgttct cgtccagcag catggcctcc tcaggctgcg cgttaagaac ttcccagtcc
     5161 gccgcgttag cggcgccggt cgcgacggcg agcaccagaa aaaaattccc aacaatcttc
     5221 tttatcacta gaacctcctc gattgaatac ctgcaacttg cgcaacccgg gcacagccag
     5281 gaatccgcac gaccataatc cgtcgtcgtt cattctttcc cagcaccgtt acacgaattt
     5341 gacaggattc gatcgctcgc gtgaatgctt gcgcgcgaag accggatcgt tggcgctgaa
     5401 acggttcaca atttatttcc gttcctgcct actcttatcg gtgctgcagg aaatccgtca
     5461 cacttggggt acgttttcgt ctccggtgcg atgcacgtcc agcagcgctt ttcccgtcac
     5521 tgtccggctc gatgccggaa ggaaaaccgc cggctcggac tgcgcggcga aaataatttc
     5581 gtctacaacg tccggcagca acacgatgca gcccgattca gtgtctgacg gaaaacctgc
     5641 tacggcgcgc ttgacctcct cgcagagacg ttttctttcg ggccccataa cctccatgat
     5701 gaaaagacca ccgtggtccc gggacaccga cgtgaggtga aacgtgcctg acgtctccac
     5761 gcccgcctgt ttcgttattt ttatgatcgc ttcttccagc agtcgctttt ttgtcatgtt
     5821 gctctcctac ggatcttatt gagt
//
LOCUS       DASQOP010000139        20344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2875183, whole genome shotgun sequence.
ACCESSION   DASQOP010000139 DASQOP010000000
VERSION     DASQOP010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 20344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20344
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2875183"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..1233
                     /locus_tag="VE030_05620"
     CDS             130..1233
                     /locus_tag="VE030_05620"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HZF22930.1"
                     /translation="MLAALVAALAAGCASTPTAAPVLERSPNASRAPAVAPVATPTTA
                     APVATAPAATAPAAGIPQTSAPPPAGAPTVSTVHKTETRVSAATEEPIIKPYREGDWR
                     PEFHVVKKGDTLYSIALDYGQDYRELAAWNNLDDPALIRLDQRLRLFPPDSVGELALP
                     QAVPLEMPPAPVAVPVKTEPKARKLPYSEQALAQLKAATAKPAGPPPASTVPSPPPPT
                     AALPAKPAATAPATPAPKEADSANVKLVWEWPTQGKLIYGFGEGTNQKGVGIEGRSGQ
                     AILASAPGKVVYSGSGLRGYGKLIIIKHNTSYLSVYAHNSQVLVKEGQSVAKGQKIAE
                     MGKSDSDRVALHFEIRRLGKPIDPLQYLPERNS"
     gene            1230..2153
                     /gene="rpoS"
                     /locus_tag="VE030_05625"
     CDS             1230..2153
                     /gene="rpoS"
                     /locus_tag="VE030_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HZF22931.1"
                     /translation="MNEENYSSQEDGVEDSPPVEQSAVDDYFGTDILSDVTQLYFNDI
                     GQNALLTVEEEVRYARLVKQGDFLSRQKMIEHNLRLVVNIAKHYTGRGLQLLDLIEEG
                     NLGLIHALEKFDPERGFRFSTYATWWIRQSIERAIMYQSRTIRLPVHVIKELNTVLRA
                     MRHLETHSECDPSADDVAHLVDFRVEDVRRLLSLNERTTSLDAPLEIDPMLSVGESIA
                     DETTPQPDIRLENAEIEIYVREWLNQLNERQRSVIERRYGLNGSEIHTLEQLAQNLNL
                     TRERVRQIQIEALASLRKILRRSGMSRETLL"
     gene            complement(2185..4137)
                     /locus_tag="VE030_05630"
     CDS             complement(2185..4137)
                     /locus_tag="VE030_05630"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HZF22932.1"
                     /translation="MSSAAGLSPINLAALLFSLALVIAPHAQHLPVWIPAFGATLLLA
                     RFHLGYRRKPLPSRWLLLAAALAAVAGVALSYRTLYGRDVGVALLTVMAALKLMEMSK
                     ARDTMVVVLLAYFLVITNFFYSQSIPTALYMLVIVWLITATMISLQHQAGPPRPAAVL
                     RHAASLIAQSVPVTLALFLLFPRVEGPLWGLPQVNYSAKSGLSDVMSPGDVSALSLSD
                     EVAFRVLFETAPEKPSQLYWRGPVLWDFDGRAWRAGQIYTAGTIEYEALGPPLNYSVT
                     LEPHDRYWMFAIDLPSRAAPNSYFSVDYQMLARRPVRARVRYDMQSVPTYRVGVNEHR
                     MQLRRALRLPDGAAPRAQALAASWRAELSSDRDIMLRALTMFREQPFIYTLAPPELGD
                     NPVDQFLFETRSGFCEHYASAFTYLMRAAGVPARVVTGYLGGEINPVDGYLMVRQSEA
                     HAWTEVWLPGEGWVRVDPTAAVSPLRIERGLAAAVPETDRASLRARNRFEWLRQARFA
                     WDAVANSWNQWVLGYNAERQVRFLSQVGLAQATWQNMVIALIATSGIILLVLALLLLV
                     RLTTLRVDPVQRAYARFCRAMARRGAPRKLSEGPRDFAARVSLQFPQLRETVQHIAAL
                     YVVLRYGGEKNPRVLEEFRQAVNSLR"
     gene            complement(4139..5173)
                     /locus_tag="VE030_05635"
     CDS             complement(4139..5173)
                     /locus_tag="VE030_05635"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZF22933.1"
                     /translation="MKRKAVAGTWFGVLGFASHLAPRASRLPLRDRIGDWIFPPKGPE
                     AGPVVLGQRRVYILPTGAGLMFAVTLVLLLIGSINYNLSLGYILTFLLAGLGVVSILH
                     TWRNLARVALRPAKTRPVFAGDSARFHIVVENPGAHQRLSLAIRLSDQNAVYFDVAPR
                     DAQDVEAGIPAARRGVLRPGRFRIFTVYPVGLFYAWANVELDLHCLVYARPEAGHVPL
                     PPPQAASGQGGASGVGEEDFAGLRTYHPGDSPRRIAWKAYARSDVFLTKQFSGSVASE
                     LWLDLADIPQTLGLEARLSRLTRWVVEAESMGQGFGLRLPGIEFEPEYGVLHRDRCLQ
                     AIALYKPARG"
     gene            complement(5170..6087)
                     /locus_tag="VE030_05640"
     CDS             complement(5170..6087)
                     /locus_tag="VE030_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZF22934.1"
                     /translation="MPNPLDPVVVQVGRIILGKERQIRLTLACLLARGHLLIEDLPGV
                     GKTTLAHALAKTLGLDFQRIQFTSDLLPADILGVSVFDRESSTFKFHPGPVFSHVILA
                     DEVNRATPKAQSALLEAMEEHQVTTDGQTRPLPEPFFVIATQNPSHQVGTFPLPESQL
                     DRFLMRIHLGYPDGAAERTLLEGRERRDMISDLAPAFTVSELLTLQKKVRDILTAPAL
                     LDYLQALIAYTRQSPDFVNGLSPRAGLFLLNAARAWAFMDGRREVIPEDVQAVLPSVA
                     GHRLYPVNGYASLGSTQLAERLIAEVAIP"
     gene            complement(6183..6767)
                     /locus_tag="VE030_05645"
     CDS             complement(6183..6767)
                     /locus_tag="VE030_05645"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZF22935.1"
                     /translation="MADKVPYPVGLVRNWTLRDGTRIVIRPIRPEDRQIERDFVRNLS
                     DESRYFRFFNALRDLSETALTRFTQVDYDKGMALIAVICENGHETEIGVARYSINPDG
                     RSCEFAIVVADAWQRRGIGSKLMHSLMDAARGRGLETMEGWVLSGNTRMLALMDGLGF
                     TVDANAGDPSLRHVVKKLATRDIPAGTAANREAT"
     gene            6892..7815
                     /locus_tag="VE030_05650"
     CDS             6892..7815
                     /locus_tag="VE030_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HZF22936.1"
                     /translation="MPTALVHHRDCALHDMGSHHPECPARITAIEDHLLATGILEHLV
                     DVDAPPATREQLLRVHDPDYVDAIEASVPQRGLVHLDPDTAMSPHSHHAALRAAGAVI
                     MATDMVMSGAVENAFCNVRPPGHHAERARAMGFCFFNNVAVGAAQALEKHGLERVAVV
                     DFDVHHGNGTEDIFRDDPRVMMVSTFQHPFYPYSGVEGRSERMVNIPLAAYSGGRQFR
                     EAVEKHWLPALGRFKPEMIFISAGFDAHRDDDMASLSLVESDYAWVTGCIKEIAEQHG
                     KGRIVSTLEGGYELTALARSVAEHIRVLSGL"
     gene            complement(7886..8254)
                     /locus_tag="VE030_05655"
     CDS             complement(7886..8254)
                     /locus_tag="VE030_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HZF22937.1"
                     /translation="MKLHLARSDNQNTFTGYGEGYVMVNAVRYERSLVVLPDLPVGAW
                     PVAGVETLREEDFEFLAGLGAEIVLVGTGDRLRFLHPRLMQSLARARVGVEVMDTRAA
                     CRTYNILVAEGRKVAAALIL"
     gene            8381..9592
                     /gene="alaC"
                     /locus_tag="VE030_05660"
     CDS             8381..9592
                     /gene="alaC"
                     /locus_tag="VE030_05660"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HZF22938.1"
                     /translation="MKDFPRIRRLPPYVFNITSELKAAARSRGEDIIDMSMGNPDGVT
                     PRHIVDKLVEASQRIDTHGYSVSKGIPRLRRAICNWYRSRFGVTLDPDSEAIVTIGSK
                     EGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGADIRSVRMTPGIDFIQELQRAIR
                     ESIPKPKMMILGFPSNPTAQCVELDFFERVVALAKDYGILVVHDLAYADICFDGWKAP
                     SIMQVLGAREVAVEFFTMSKSYNMAGWRVGFMVGNRELVSALARIKSYHDYGSFTPIQ
                     VASIAALEGPQDCVREICEKYQKRRDVLARGLHEAGWMVQVPKASMYIWAEIPAPYKE
                     MGSLEFAKKLLADAKVAVSPGIGFGEYGDSHVRFALIENEARTRQAVRGIKEMFRRDG
                     LLMPGPRLVSA"
     gene            9700..10155
                     /locus_tag="VE030_05665"
     CDS             9700..10155
                     /locus_tag="VE030_05665"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZF22939.1"
                     /translation="MAADALRIVQITGKNGEVLEREWLAGAEAVHRQLRPQLPADYSA
                     KMQRIFRDGGEMCVAVRNKKVVGVAVYREFENTHAGRRFYVDDLVTDESERSSGVGRA
                     LIVYLQQVARERGCPGLDLESGTQRMRAHRFYFREGFFITSFSFRKDFQ"
     gene            10152..11468
                     /locus_tag="VE030_05670"
     CDS             10152..11468
                     /locus_tag="VE030_05670"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HZF22940.1"
                     /translation="MKAIHVGLLGIGTVGGGTFAVLRRNQEEISRRAGRGIVIKMVAD
                     KDLGRARKVVGDAAEITDDAYKVVRNPEIDIVVELIGGTKVAMDLILAAIDGGKHVVT
                     ANKALLATHGNEIFLAAQKKGVMVAFEAAVAGGVPIIKALREGLTANRIEWIAGIING
                     TSNFILSEMREKGSSFKEVLAEAQRLGYAEADPTFDIEGTDAAHKLTIMAALAYGIPM
                     QLDKAYTEGISGLTKEDIRYAGELGYRIKLLGITRRTPAGIELRVHPTLIPSRRLIAN
                     VEGVMNAILVKGDAVGATLYYGAGAGAEPTASAVVADLVDVTRMHTADPEHRVPHLAF
                     QPDRMSNTPILPMEEVETSYYLRLRVLDRPGVLADITRILADLSISIDAMVQKEPSEG
                     QEQVDIIMLTHMTQEKNINSAIKRIELLPVVAGKITRIRLEQLGRD"
     gene            11679..12803
                     /gene="thrC"
                     /locus_tag="VE030_05675"
     CDS             11679..12803
                     /gene="thrC"
                     /locus_tag="VE030_05675"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004674289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HZF22941.1"
                     /translation="MTAHYTGLINRYRDRLPVGPNTPVITLNEGNTPLIELTNLPKIL
                     RKDVRIFCKFEGLNPTGSFKDRGMTVAVTKAVEAGARAIICASTGNTSAAAAAYAARA
                     GITCYVLIPEGKIALGKLSQAMMHGSVVIQIRGNFDDGMRLVQEVAQSMPLNIVNSIN
                     PYRLQGQKTIAFEVIETLGDAPDYHALPVGNAGNISAHWTGYSEAVGKNTASCPHCDG
                     DCSYLTKSIATHRPIMLGYQAAGAAPFLRGAMVDEPETIATAIRIGHPVSWKLAWAAK
                     SESGGWFNECTDEEILRAQKLLAETEGIFCEPASGASVAGVLKDLELGVIGEGTTIVC
                     TLTGHGLKDPDSAIAQSIKPVVVDATRAAIERVISENLPR"
     gene            complement(12844..13677)
                     /gene="nadC"
                     /locus_tag="VE030_05680"
     CDS             complement(12844..13677)
                     /gene="nadC"
                     /locus_tag="VE030_05680"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HZF22942.1"
                     /translation="MDLAPVIRSDVARALAEDIGSGDVTASLVPRNQAAHAHVVTREA
                     AVLCGCAWFDEVFRQIDPETRVNWRAKDGDRVSPNQMLCEIDGRARSLLTGERAALNF
                     LQTLSGVATRTRTFVDAVAGTKATILDTRKTLPGLRQTLKYAVRCGGASNHRMGLYDG
                     VLIKENHIAATGGIGPALRQASQVAGNVPIQIEVESIAQLNAAIEAGAKLILLDNFDL
                     AGLREAVKVTAGRAALEASGGVNLDTVRAIAETGVDRISIGSLTKDVRAVDLSMRFAP
                     A"
     gene            13724..13903
                     /locus_tag="VE030_05685"
     CDS             13724..13903
                     /locus_tag="VE030_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22943.1"
                     /translation="MSRLMGVVSYGIGTIPGLILVGLAVEEKYLRVANYAINMLYPYP
                     KTGLEFRLPVVQTVT"
     gene            complement(13896..14570)
                     /locus_tag="VE030_05690"
     CDS             complement(13896..14570)
                     /locus_tag="VE030_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011402229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HZF22944.1"
                     /translation="MDSSPALKALVFDAYGTLFDVHSVIAECEALFPGTGAALSQLWR
                     GKQLEYTWLRSLMGRYADFSAITRAALTTSCNMLRLELSEAAARRLMDAYLVLKAFPD
                     VVDTLSRLRGRKLAILSNGSPDMLNAVVRHAGLDQLLDAVISVDALSIFKPHPSVYAY
                     AAQKLQAPAGAIGFVSSNFWDVAGATSFGFRTFWINRAGGAPDDLGYQPAAVLSRLSE
                     LPALLK"
     gene            complement(14570..14842)
                     /locus_tag="VE030_05695"
     CDS             complement(14570..14842)
                     /locus_tag="VE030_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZF22945.1"
                     /translation="MKNLRLVPILVLGTLLPPAVADDLTVAAHATASGLMLWSQHRGA
                     SGFDRLLDDGVTIWFAGESLHAISAPGHAPGSTCYPSRARLRSGAG"
     gene            15008..17614
                     /gene="clpB"
                     /locus_tag="VE030_05700"
     CDS             15008..17614
                     /gene="clpB"
                     /locus_tag="VE030_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HZF22946.1"
                     /translation="MRFDKLTTRFQQALADAQSKAVGQDNPYIEPQHLLLALLEQDDG
                     GTASLLARAGVNVPPLKEALKASIGRLPKVEGQGGEISVSRELTSLLNLTDKEAQKRG
                     DQFIASELFLLALADDKGETGKLLKSHGLNRKALEQAIEAVRGGEAVQSQEAEGSREA
                     LKKYTLDLTERARIGKLDPVIGRDDEIRRVIQILQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINEEVPETLKGKRVLSLDMAALLAGTKYRGEFEERLKSVLKEISQEEGRVIVF
                     IDELHTMVGAGKAEGAMDAGNMLKPALARGELHCVGATTLDEYRKYIEKDAALERRFQ
                     KVLVDEPSVESTIAILRGLQEKYELHHGVEITDPAIVAAAELSHRYITDRFLPDKAID
                     LIDEAAARIKMEIDSKPEQMDKLDRRIIQLKIEREAVKKEKDEASRKRLKALEEEIKR
                     LEKEYSDLEEIWKAEKAQVQGSQHIKEELEKLKLEMEAAKRKGDWQKVSELQYGKIPQ
                     LESQLKKAETPADQPKFRLLRTQVGAEEIAEVVSRATGIPVSKMMQGEREKLIKMEEH
                     LHQRVVGQDEAVRLVADAIRRSRAGLADPNRPYGSFLFLGPTGVGKTELCKALAEFLF
                     DSQDHLIRIDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRKPYSVILLDEVEK
                     AHPDVFNVLLQVLDDGRMTDGQGRTVDFKNTVIVMTSNLGSQMIQQMAGDDYQLIKLA
                     VMGEVKSYFRPEFINRIDEVVVFHALDEKHIKSIAKIQLGHLEKRLAAMDLKLEVDDG
                     VLAELAHVGFDPVFGARPLKRAIQATIENPLAKEILEGRFAPKDVIRVTSAGGKLRFE
                     KGKQAAHAVGGS"
     gene            17762..18562
                     /locus_tag="VE030_05705"
     CDS             17762..18562
                     /locus_tag="VE030_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HZF22947.1"
                     /translation="MTVESPAEPLALFRGEPVHELPGDLYIPPDALRVFLETFEGPLD
                     LLLYLIRKHNLDVLDIPMASLTRQYMEYVEVMRQHQLELAAEYLLMAALLIEIKSRML
                     LPRPTQVSPEEEDPRAELMRRLLEYERMKLAGHQLNELPLAGRDFALVQVWLDREVEQ
                     RLPDVNPEDLRNAWLGLIARARVTRHHRITREQLSVREHMSRILRSLQHLRFVRFEDL
                     FDETAGVPVLVVSFLAILELARESLVQVTQQGAFSPIYVKLNHGITSV"
     gene            18543..19064
                     /gene="scpB"
                     /locus_tag="VE030_05710"
                     /pseudo
     CDS             18543..19064
                     /gene="scpB"
                     /locus_tag="VE030_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212744.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
     gene            19247..19873
                     /locus_tag="VE030_05715"
     CDS             19247..19873
                     /locus_tag="VE030_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008115777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZF22948.1"
                     /translation="MRAVLVANPKGGSGKTTVATNLAGSLATRGEKVYLWDLDRQQSS
                     RNWLTIRPADRPAIHRLDARDDHEPSMPRGSSWLVLDSAAGLHGKNLAHALKLAQKVL
                     VPVQPSVFDMAATSTFLQTLMEEKAIRKHKTFVGIVGMRVDPRTKAAATLEAFLAQFK
                     LPVLTYLRDTQVYVNAAFNGLSVFDLPDYLSARDIEQWQALLAWLEED"
     gene            19980..20056
                     /locus_tag="VE030_05720"
     tRNA            19980..20056
                     /locus_tag="VE030_05720"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20014..20016,aa:Met,seq:cat)
BASE COUNT         4220 a         6083 c         6414 g         3627 t
ORIGIN      
        1 ggtgctggtc gagcgcggcc gcgacggatt cagccagtcg gtgctggacg acgtcaattt
       61 cgttccgctg attgcggggg tttcggcttg actatcgttt tccgctgcgg ggaattttcg
      121 cggccgctca tgcttgcggc attggttgcc gcgctcgccg cgggttgcgc atccacgcct
      181 actgcggcgc cggtgctcga gcgttcgccg aatgcgtccc gcgcgccggc cgtagctccg
      241 gtcgccacac ccacgacagc cgcacctgtg gccaccgctc cggctgcgac cgctccggct
      301 gccggcatac cccagacgtc tgcacccccc cctgcagggg cgccaaccgt ctcgactgtc
      361 cacaaaacgg agacacgggt ttccgcagcc acggaagagc cgatcatcaa accctatcga
      421 gaaggcgatt ggcgcccgga attccatgtc gtcaagaaag gcgacacgct ctacagcatc
      481 gcgctggatt acgggcagga ttaccgtgag ctcgcggcct ggaacaatct ggacgatccg
      541 gcgctgattc gtttggatca acgtttgcgg ctgttcccgc cggacagcgt cggagaactg
      601 gcgctgccgc aggccgtgcc gctggagatg ccgcccgcgc cggttgcggt accggtcaag
      661 accgagccga aagcacgcaa gctgccttac tcggaacagg cgctggcgca gttgaaagcg
      721 gcaacagcga agccggccgg gccaccgccc gcgtcaacag tgccgtctcc gccgccgccc
      781 acggcagcgc ttcccgccaa gcctgcagcg acggcgccgg caacgcctgc gcccaaagaa
      841 gccgacagcg cgaacgtgaa gctggtttgg gagtggccga cccagggaaa attgatttac
      901 ggtttcgggg aaggaacgaa ccagaagggc gtgggaatcg aaggccgctc agggcaggcg
      961 atactcgcaa gtgcgccggg aaaggtcgtg tacagcggca gcggcctgcg cggttacggc
     1021 aaactgatta tcatcaagca taatacgtcg tacttgagcg tgtacgctca caatagccag
     1081 gtcctggtga aggaagggca atcggtggcc aagggccaga aaattgcaga aatgggcaag
     1141 tccgacagcg accgggtcgc cctgcatttc gaaatccgca ggctcggcaa accgatcgac
     1201 ccgctgcagt atctgcctga gcggaattca tgaacgagga gaattactct tcacaggaag
     1261 acggcgtcga ggacagcccg cccgtcgaac agtccgctgt cgatgactat ttcggcaccg
     1321 acatcctcag cgacgtcacg cagctctatt tcaacgacat cgggcagaac gcgctgctga
     1381 ccgtagagga agaggtcagg tacgcgcggc tggtcaagca aggggatttc ctgtcacgcc
     1441 agaagatgat cgaacacaat ctgcggctgg ttgtgaacat cgccaagcac tacaccggcc
     1501 gtggactgca actgctggac ctgatcgaag aaggcaatct cggcctgatc cacgcgctcg
     1561 agaaattcga tcccgaacgc ggattccgct tctccaccta tgccacttgg tggatccgcc
     1621 aaagcatcga acgcgccatc atgtaccagt cgcgcaccat ccgcctgccc gtgcatgtaa
     1681 tcaaggaact gaacaccgtg ctgcgggcga tgcgccatct cgagacccat tccgagtgcg
     1741 accccagcgc ggatgatgtt gcgcatctgg tggacttccg ggtcgaggac gtccgacgcc
     1801 tgctgtcgct caacgagcgc acgacgtcgc tggatgcgcc cctggagatc gatcccatgc
     1861 tttcggtcgg cgaatcgatc gccgacgaaa ccaccccgca gcctgacatc aggctggaga
     1921 acgcggaaat cgagatctac gtgcgcgagt ggctgaatca gttgaacgaa cgccaacgca
     1981 gcgtgatcga gcggcgctac gggctaaacg gttccgagat tcataccctc gagcaactgg
     2041 cgcaaaattt gaacctgacc agggaaagag tgcggcagat ccagatcgag gcgctcgcct
     2101 cgctgcgcaa gattctgcgc cgcagcggaa tgtcgaggga aaccttactc tagcgatcgg
     2161 tgccaggccg cgctcgactg cagttcacct caggctgttc accgcctggc ggaattcctc
     2221 cagaacgcgc ggattctttt cgccgccgta acgcagcacc acgtacagcg cggcgatgtg
     2281 ctgaaccgtc tccctcagtt gcggaaactg cagcgacacc cgggccgcga agtcacgtgg
     2341 tccttcgctg agcttgcgcg gtgcgccgcg ccgggccatc gcccggcaga atcgggcata
     2401 agcgcgctgc accggatcca cgcgtagcgt ggtgagccgc acaagtagca gcaacgccag
     2461 caccagcagg atgatgccgc tggtggcaat gagcgcgatc accatatttt gccaggttgc
     2521 ctgtgccagt ccgacttgcg acaggaaacg cacctgcctc tccgcgttgt agcccagcac
     2581 ccattggttc cacgagttgg cgacggcgtc ccacgcgaaa cgggcctgac gcagccattc
     2641 gaaacgattg cgcgcccgca acgatgcccg atcggtttcc ggtaccgccg cagccagtcc
     2701 gcgttcgatg cgcaacggtg aaaccgccgc cgtcgggtcg acccgcaccc aaccctcgcc
     2761 gggcagccaa acctcggtcc aggcgtgcgc ctcggactgg cgcaccatga ggtaaccgtc
     2821 cacgggattg atctcgccgc ccaggtagcc ggttaccacg cgcgcaggaa cgccggcggc
     2881 gcgcatgagg taggtgaatg ccgaagcgta atgctcacag aagccgctac gggtttcgaa
     2941 caggaactga tcgacaggat tgtcaccgag ttccggcggc gccagtgtgt agatgaacgg
     3001 ctgttcccgg aacatcgtca gcgcccgtag catgatgtcg cggtcgctgg acaattcggc
     3061 gcgccaggaa gcggccagcg cctgcgcccg cggagcggcg ccatccggaa gccgaagcgc
     3121 gcgacgcaac tgcatcctat gctcattgac ccccacacgg tacgttggta cggactgcat
     3181 gtcgtagcgg acacgtgcgc gcaccggccg tcgcgccagc atctggtagt ccacggaaaa
     3241 gtaactgttg ggtgcggcgc gcgacggcag gtctatggcg aacatccagt accggtcgtg
     3301 aggttcaagc gttaccgaat agttaagcgg cggacccagc gcctcgtatt cgattgtacc
     3361 ggcggtgtaa atctggccgg cacgccaggc gcggccgtcg aaatcccaca gcactggtcc
     3421 gcgccagtag agttgcgagg gtttttccgg cgctgtttcg aacaacacac ggaaagcaac
     3481 ctcgtcggac aggctgagcg cgcttacgtc accgggagac atgacgtccg acaaacccga
     3541 cttggcgctg tagttgacct gaggcaatcc ccagagcggg ccctccaccc gcggaaataa
     3601 caggaagagc gcaagcgtga caggaacgct ttgcgcgatc agactggctg catggcgcag
     3661 caccgccgcc ggcctgggtg ggcccgcctg gtgctgaagg ctgatcatgg tcgcggtgat
     3721 gagccagacg atgaccagca tgtacaacgc ggtgggaatc gactgcgagt agaaaaaatt
     3781 ggtgatgacc aggaagtagg cgagcagcac cacgaccatg gtgtcgcgcg cctttgacat
     3841 ctccatcagt ttcagagccg ccatcacggt caacagcgcg actcccacgt cgcggccgta
     3901 aagcgtccga tatgacaggg ccacgccggc gacggcggcc agcgcggcgg caagcagcag
     3961 ccagcgactg ggcaacggct tgcgccggta gcccagatgg aaacgtgcca gcagcagcgt
     4021 ggcgccgaaa gccggaatcc agaccggcag atgctgcgcg tgcggcgcaa taaccagtgc
     4081 cagcgagaac agcagcgcgg cgagattgat cgggctcaag ccggcggcgc tcgacatgtc
     4141 atccgcgcgc cggcttgtac aacgcgatcg cctgcaggca acgatctcga tgcaatacac
     4201 cgtactcggg ctcgaactcg attccgggca ggcgcagacc gaagccttgg cccatggatt
     4261 ccgcctcgac aacccagcgg gtgagccggg aaagcctggc ttccagaccc agcgtttgcg
     4321 gaatgtcggc gagatccagc cacaactccg aggccacgct tccggaaaac tgcttggtga
     4381 gaaatacatc actgcgcgcg tacgccttcc aggcgatgcg acgtggagaa tcaccgggat
     4441 gataggtgcg caggccggca aaatcctctt cgccaacgcc gctggccccg ccttgcccgc
     4501 tggcggcctg cggcggcggc agcggaacat ggccggcctc ggggcgcgca tacaccaggc
     4561 agtgcaagtc aagttccaca ttggcccagg cgtagaacag gccgaccgga tagaccgtga
     4621 aaatgcgaaa acgcccgggc cgcagcacac cgcgtcttgc ggcggggatg cctgcttcca
     4681 cgtcctgcgc atcacgcggc gccacgtcga agtacaccgc gttctggtcg gacagccgga
     4741 tcgccaggct cagccgctga tgcgcgcccg gattctcgac cacgatatga aagcgggcgg
     4801 aatctccggc gaaaaccggc cgcgttttgg cggggcgcag cgcgacgcgc gccaggttgc
     4861 gccaagtatg caggatcgac accacgccga ggccggcgag caggaaggtg aggatatatc
     4921 cgaggctgag gttgtaattg atcgagccga tcagcagcaa caccagggtg acggcgaaca
     4981 tgagcccggc gccggtcggg agaatgtaga cacgccgctg gccgagtacc accggccccg
     5041 cttccggtcc cttgggcggg aagatccagt caccgatccg gtcgcgaagc ggaaggcgcg
     5101 aggcgcgagg cgcgaggtgt gaggcgaacc ccaatacgcc gaaccaagta cctgccacgg
     5161 ccttgcgctt cacggaatgg cgacttccgc gatcagccgc tcggcaagtt gcgtgctgcc
     5221 caatgaggcg taaccgttga caggatagag ccggtgcccg gccacgctcg gcagcaccgc
     5281 ctgcacgtct tccgggatga cttcgcggcg cccatccatg aatgcccagg cgcgggcggc
     5341 gttcagcagg aacaagcccg cgcgcggcga cagtccgttg acaaaatccg gggactggcg
     5401 ggtataggcg atcagcgcct gtaggtaatc cagcagcgcc ggcgcagtca ggatatcgcg
     5461 aacttttttc tgcaacgtga gcagttccga cactgtgaat gccggcgcca ggtcggaaat
     5521 catgtcgcgg cgctcgcgtc cctccagcag cgtgcgctcg gccgcgccgt ccggatatcc
     5581 cagatgaatg cgcatcagaa aacggtcgag ctgcgattcc ggcaatggaa atgtgccaac
     5641 ctggtgcgac gggttctggg tggcgataac gaaaaacggc tcgggcagcg gccgggtctg
     5701 gccatcggtg gtgacctgat gctcttccat cgcttccagc agcgcgcttt gtgcttttgg
     5761 cgtggcgcgg ttgacctcgt cggcaaggat cacgtgcgag aacaccgggc cgggatggaa
     5821 tttgaaggtc gagctctcgc gatcgaacac cgataccccg agtatgtctg cgggcagcag
     5881 atcgctggtg aattggatgc gctggaaatc gaggcccagg gtcttggcca gcgcgtgcgc
     5941 gagcgttgtc ttgcctactc ccggcaggtc ttcgatcagc agatgcccgc gtgcgagcag
     6001 gcaggcgagc gtgaggcgga tctggcgttc cttgccgagg atgatgcggc cgacctgaac
     6061 gacaacggga tcgagcggat tgggcatgcg tcagggatgg atatctgaat gcgaattccg
     6121 cgaagccatg ctcgcatggg aacgtgcggc cggtccggag ttcccggaaa ccctagttta
     6181 cctcaggtcg cttcccggtt cgcggccgtt ccggccggaa tgtcgcgcgt cgccagtttc
     6241 ttgactacat gccgcagcga cgggtcgccc gcgttcgcgt cgacagtgaa accaagtcca
     6301 tccatcaggg ccagcattct ggtattgccc gacaggaccc agccttccat tgtttcgagc
     6361 ccacggccgc gggcggcgtc catcaggctg tgcatgagtt tcgagcctat gcccctgcgc
     6421 tgccaggcat cggcaacgac gattgcgaac tcgcagctcc tgccgtccgg attgatggag
     6481 taacgggcta cgccgatttc cgtttcgtgg ccgttctcgc agatgaccgc aatcagcgcc
     6541 atccccttgt cgtaatcgac ctgtgtgaag cgcgtgagcg ccgtttcgct gagatcccga
     6601 agagcattga aaaaacgaaa atagcgcgat tcgtcggaga ggttgcgcac aaagtcccgc
     6661 tcgatctggc gatcctccgg ccgaatcgga cgaatgacga tacgagtgcc gtcgcgcagc
     6721 gtccaattcc gcaccagtcc gacagggtag ggtactttat cagccacgtg cagacgcaag
     6781 ggggtaaatg ctcaaaggtt agcccaatgc aaatcccgcg tcatggacaa gcgcggtggc
     6841 cctgaataag atgcggcgag gcccggattc ccgttaccgc ccgccaacat tatgccgacc
     6901 gcccttgttc accatcgaga ctgcgcgctg cacgacatgg gctcccatca ccccgagtgt
     6961 ccggcgcgga taactgcgat cgaagatcac ctgctcgcca ccggaattct ggagcatctg
     7021 gtggatgtgg acgcgcctcc ggcgacgcgc gagcagctgt tgcgggtaca tgatcccgac
     7081 tacgtggatg cgatcgaagc cagcgtcccg cagcgcggac ttgtccatct cgatcccgat
     7141 accgcgatga gcccgcactc gcatcatgcc gccctgcggg ctgcaggtgc ggtgatcatg
     7201 gcgaccgaca tggtgatgag cggcgcggtc gagaacgcct tctgcaacgt gcgtccaccc
     7261 gggcatcatg ccgaacgcgc tcgcgcgatg ggattctgct ttttcaacaa cgtcgcggtg
     7321 ggcgcggccc aggcgctgga aaagcacggt ctggagcgcg tggcggtggt ggacttcgac
     7381 gtgcaccacg gcaacggcac cgaggatatc tttcgcgatg atccccgggt aatgatggtc
     7441 tccacgtttc agcatccgtt ctatccttac agcggggtgg agggacgttc cgagcgcatg
     7501 gtaaacattc cgcttgcggc atacagcggt ggccggcagt ttcgcgaagc ggtcgaaaag
     7561 cactggctgc ctgcgttggg tcgcttcaag ccagagatga tattcatttc cgccgggttt
     7621 gatgcccacc gggacgacga catggcgtcg ttgagcctgg tggaaagcga ttacgcctgg
     7681 gtgaccgggt gcatcaagga aattgccgaa caacacggca aagggcggat cgtttcgact
     7741 ctggagggcg gctacgaatt gaccgcgctg gcacgcagcg tcgccgagca tatccgcgtg
     7801 ctgagtggac tgtaaagaaa atactgggct gggccgaccg ggatgaattc cgattcgtgt
     7861 ccggtaaatg aaagattcgg cagacttaga gaattaacgc tgcagcgact ttacggcctt
     7921 cggccaccag gatgttgtag gtccggcagg ccgcgcgcgt atccatcacc tcgaccccga
     7981 cgcgagcccg cgccaggctt tgcattaaac gggggtgaag gaagcgcagc ctatctccag
     8041 tgccgacaag cactatttcc gcgcctaagc cggcgagaaa ctcgaaatcc tcttcccgca
     8101 gcgtttctac gccggcaaca ggccaagccc cgactggcag atcgggcagg accacgagac
     8161 tgcgctcgta gcgcactgcg ttcaccatca cgtagccctc accgtaaccg gtgaaggtat
     8221 tctggttgtc gcttctggca aggtgaagtt tcaaggcgcg caggttcttt gccgcaatgc
     8281 ggatgatccg gtaggattat aacttttcaa tcgccccgac ttccatcgct tgtacagtcg
     8341 gttacgaggc gccacccagc gccggtccga acccatgcct atgaaggatt tcccccgcat
     8401 acgacgcctg cctccctacg tcttcaacat caccagcgag ttgaaggcgg cggcacgaag
     8461 ccgtggcgaa gatatcatcg acatgagcat gggcaatccg gacggggtga cgcccaggca
     8521 catcgtggac aagctggtgg aggcgtcgca acgcatcgac acccacggct attcggtctc
     8581 caaaggtatt ccgcggttgc gccgcgcgat ttgcaactgg tatcggtccc gctttggcgt
     8641 gacgctggac ccggattccg aggccatcgt cacgatcggc tcgaaggagg gcctcgcgca
     8701 tctgatgctc gccacgctgg atcacggcga cactgtgctg gtgccaaatc cgagctaccc
     8761 gattcatatt tacggcgcgg tgatcgccgg cgcggatata cgctcggtgc gcatgacgcc
     8821 ggggatcgat ttcatccagg agctgcaacg ggcgatccgg gaatccatcc ccaagccgaa
     8881 gatgatgatc ctgggtttcc cgagcaaccc gaccgcgcaa tgcgtggaac tcgatttctt
     8941 cgagcgcgtc gtggcgctgg cgaaggatta cggcatcctg gtggtgcacg atctggccta
     9001 cgccgacatc tgcttcgacg gctggaaggc gccatcgatc atgcaggtgc tcggtgcccg
     9061 tgaagtcgca gtcgaattct tcaccatgag caagagctac aacatggccg gctggcgcgt
     9121 cggtttcatg gtcggcaaca gggaactggt gtccgcgctg gcacgcatca agagctacca
     9181 tgactacggc agcttcacgc ccatccaggt ggcatccatc gccgcgctcg agggcccgca
     9241 ggactgcgtg cgcgagatct gcgagaaata tcagaaacgc cgcgacgtgc tcgcacgggg
     9301 tctgcatgaa gccggatgga tggtgcaggt gccgaaagcg tcgatgtaca tctgggcgga
     9361 gattccggct ccttacaaag agatgggatc gctcgagttc gccaagaaac tcctggcgga
     9421 cgccaaggtg gcggtttccc ccggcatcgg ctttggcgag tatggcgaca gtcacgtgcg
     9481 cttcgcgctg atcgagaacg aagcgcgcac ccgccaggcc gtgcggggga tcaaggaaat
     9541 gttccgccgc gacggattgc tgatgccagg cccgcgcctc gtttccgcat aggcatacgc
     9601 gcccgggcca gcgtgacatt gcttacggcg ggacggattt ttcaggtttc cgatccgatc
     9661 gggcgcgcgc cgcccgatct ttttatgtag agattgatca tggcagccga cgccttgcgc
     9721 atcgtgcaga tcaccgggaa gaatggcgaa gtgctggagc gcgagtggct cgccggcgcc
     9781 gaggccgtgc atcggcaact gcgcccgcaa ctgcctgccg attattccgc caagatgcaa
     9841 cggattttcc gtgacggggg cgaaatgtgt gtcgcggtga gaaacaagaa agttgtcggc
     9901 gtggcggtct accgcgagtt cgaaaatacg catgccggcc gccggttcta cgtcgatgat
     9961 ctggtgacgg atgaaagcga gcgttcctcc ggagtcgggc gggcgttgat cgtttatctt
    10021 caacaggtcg cccgtgaacg cggctgcccg ggattggacc tggaatcagg gacgcagcgc
    10081 atgcgcgcgc atcgttttta cttccgcgag ggattcttca tcacctcatt ttcattcaga
    10141 aaagattttc aatgaaagcg atacacgtgg gcctgctcgg catcggcaca gtgggcggcg
    10201 gaacgttcgc cgtgctccgc cgcaaccagg aagagatcag ccggcgggcc ggccgcggca
    10261 tcgtcatcaa gatggtcgcc gacaaggacc tgggcagggc gcgcaaggtc gttggcgatg
    10321 ccgccgagat caccgatgac gcatacaagg tggtacgcaa tcccgaaatc gacatcgtgg
    10381 tcgaattgat cgggggcacc aaagttgcga tggacctgat tctggcggcg atcgacggcg
    10441 gcaagcacgt cgtaaccgcc aacaaggcgc tgctggcgac acatggcaac gagatttttc
    10501 tggccgcgca aaagaaagga gtcatggtcg ccttcgaagc ggcggttgcc ggaggcgtgc
    10561 cgatcatcaa ggcgcttcgt gagggcctga ctgccaaccg catcgagtgg atcgccggaa
    10621 tcatcaacgg gacctcgaac ttcatcttgt cggagatgcg cgagaagggt tcgagcttca
    10681 aagaggtgtt ggccgaggcg cagcgcctgg gatatgcgga agccgatccg acgttcgata
    10741 tcgagggtac ggatgccgcg cacaaactca ccatcatggc ggcgctcgcg tacggcatcc
    10801 cgatgcagtt ggacaaggcc tatacagagg gcatttccgg tcttaccaag gaggatatcc
    10861 gctacgccgg ggaactcggc taccggatca agttgctcgg catcacccgg cgcacgccgg
    10921 ccgggatcga attgcgggtg catcctaccc tgatcccttc gcggcgactg atcgctaacg
    10981 tggagggggt catgaacgcc atcctggtca agggcgacgc ggtcggggcc acgctgtact
    11041 acggtgccgg tgccggcgcc gagccgaccg cctcggcggt ggtggccgac ttggtggacg
    11101 tgacccggat gcataccgcc gacccggagc accgggtgcc ccatctggcg tttcagccgg
    11161 accggatgtc caatacaccg attctgccaa tggaggaagt cgagacctcg tactacctgc
    11221 ggctgcgggt gctggatcgt cctggtgtcc tggctgacat cacccgcatc ctagccgacc
    11281 tgtccatctc gatcgatgcg atggtgcaga aggagccgag cgagggccag gaacaggtgg
    11341 acatcatcat gctgacccat atgacccagg aaaaaaacat caactccgcg atcaagcgca
    11401 tcgagttact gccggtggtg gccggcaaaa ttacccgcat ccgcctcgaa caactcggtc
    11461 gcgactaggg ccagatcctt caacgaggcg ccaaggcgca aaggatgcgc aaagaaatgc
    11521 attaacgaga atgttcggcg ataggaagag ccaacgcacg gccgccacga aaatgctctg
    11581 ggaattgttt cgacatccgg aaggaatttg ctttcgattt tctttgcgtt acctttgcgt
    11641 cttcgcgcct ttgcggttaa ccatttttcc gaataaacat gacagctcac tacaccggcc
    11701 tcatcaaccg ctaccgcgac cgactcccgg tcgggcccaa tacgcccgtc atcacgctca
    11761 acgaaggcaa cacgccgctg atcgaactca caaatctgcc gaagatcctc aggaaggacg
    11821 tgcgcatctt ctgcaagttc gaagggctga atccgaccgg ttcgttcaag gaccgcggca
    11881 tgaccgtggc ggtgacgaag gcggtggaag caggggctcg cgcgatcatc tgtgcttcca
    11941 ccggcaatac gtcggcggcc gcggctgcct acgccgctcg tgcaggcatc acctgctatg
    12001 tgttgatacc agaaggcaag attgcgctcg ggaagctctc gcaggcgatg atgcatggat
    12061 cggtggtcat ccagatccgc ggcaatttcg atgacggcat gcggctggtg caggaagtgg
    12121 cgcagagcat gccgctgaac atcgtcaact cgatcaatcc ctatcggctg cagggccaga
    12181 aaaccatcgc cttcgaggtg atcgagacgc tgggcgacgc gccggattat cacgcgctgc
    12241 cggtcggcaa tgccggcaac atctcggcgc actggacggg ctacagcgag gcggtgggca
    12301 agaacaccgc gagttgcccg cactgcgacg gcgattgcag ttacctgacc aagtccatcg
    12361 ccacccatcg gccgatcatg ctcggctacc aggcggcggg cgccgcaccg tttctgcgcg
    12421 gtgcaatggt ggatgagccc gagaccattg ccaccgcaat ccgcatcggc catccggtat
    12481 cgtggaaact ggcgtgggca gcgaagagcg aatccggggg ctggttcaac gagtgcaccg
    12541 acgaagagat cctgagggca cagaaactcc tggcggaaac ggaaggaatt ttctgcgagc
    12601 cggcctctgg ggcctcggtt gccggtgtac tgaaggacct ggaactcggc gtgatcggcg
    12661 aaggtacgac aattgtgtgc acgttgaccg gccacggact gaaagacccg gacagcgcga
    12721 ttgctcagag catcaaacct gtcgtagtgg acgccacgcg tgcggcgatt gagcgcgtga
    12781 tcagcgagaa tttgcctcgg tagtacaagg atttccgccc ttttatgcga agcgccaggc
    12841 tgatcaagcg ggcgcaaaac gcatcgatag atccactgcg cgcacgtcct tggtaagcga
    12901 cccgatggat atgcggtcca ctccggtttc cgcgatggcc cgcacggtgt cgagattcac
    12961 tccgcccgaa gcctccagcg ccgcgcgtcc cgcggtcacc ttcacggctt cccgcaagcc
    13021 cgcgagatcg aagttgtcga gcaggataag cttggcgccc gcttcgattg ccgcgttcaa
    13081 ctgagcgatc gattccactt cgatctggat cgggacattc ccggcaactt gactggcctg
    13141 ccggagcgcc gggccgattc ctccagtcgc ggcgatgtgg ttttccttga tcagaacccc
    13201 gtcatataga cccatgcgat gattgctggc accgccgcag cgcaccgcgt acttgagcgt
    13261 ctggcgcagc cccggcagcg tcttgcgggt atcgaggatg gtcgctttcg ttcccgcaac
    13321 ggcatccacg aacgttcgcg tgcgagttgc cacgccggac agggtctgca ggaaattgag
    13381 cgcagcgcgt tcgccggtga gcaggctgcg ggcgcggccg tcgatttcgc acagcatctg
    13441 attcggggac acgcggtcgc cgtctttggc gcgccagttc acccgcgtct ctgggtcgat
    13501 ctggcggaac acttcatcga accacgcaca accgcataac accgccgctt cgcgggtaac
    13561 gacatgcgca tgggcagcct ggttcctggg caccaggctg gcggtaacgt cgccgctgcc
    13621 gatgtcttcc gccagcgccc tggcaacgtc gctcctgatc accggtgcca aatccatgcg
    13681 attcccagaa actgaaggcg gcattctatc gcgggaggcg ttgatgtcgc gcttaatggg
    13741 tgtggtctcg tacggaatcg gcaccattcc cggcctcatc ctggtcggtc tggcggtgga
    13801 agaaaagtat ctgcgggtgg cgaactacgc gatcaacatg ctgtatccct atccgaaaac
    13861 cggactggag ttcaggctgc cggtggtcca gaccgtcact tgagcagggc gggcagttcg
    13921 ctgagccgtg atagcactgc ggccggctga tagccgaggt cgtcgggtgc acctcccgcg
    13981 cggttgatcc agaaggtgcg gaatccgaac gatgtggcgc cagccacatc ccagaagttc
    14041 gaggacacga aaccgattgc gcctgccggc gcctgcaatt tttgcgcggc gtaagcgtag
    14101 acgctcggat ggggtttgaa gatactcaac gcatcgacgc tgatcacggc gtcgagcagt
    14161 tggtcgagcc cggcgtgtcg aactacggcg ttcagcatat ccggtgaccc attggacaga
    14221 atggcgagct tgcgcccgcg gagccgactg agcgtgtcca cgacgtccgg gaaggccttc
    14281 aataccaggt aagcatccat cagccgcctc gccgcggctt cgctcagttc cagacgcagc
    14341 atgttgcacg aggtcgtcag cgcggcgcgg gtgatcgcgg aaaaatcggc gtagcggccc
    14401 atcaggctgc gcagccaggt gtattcgagc tgtttcccgc gccatagttg ggaaagcgcc
    14461 gcgcccgttc ccgggaataa cgcttcgcac tcggcgatca ccgaatgtac gtcgaacaaa
    14521 gtcccgtagg catcgaaaac aagcgctttc agcgcgggag acgaatccac tagcctgccc
    14581 cgctgcgcag gcgggcgcgg gacggataac aagtgctccc tggagcgtgg ccgggggcag
    14641 agatggcgtg cagcgattcg ccagcaaacc agatggtgac gccgtcgtcg agcagccgat
    14701 cgaagccgct ggcgccgcgg tgctgactcc atagcatcag gccggacgca gtggcgtgtg
    14761 cggcgacggt caagtcgtcg gccacggccg gtggcaacag cgtgcccagc acgagtatcg
    14821 ggaccagacg gaggttcttc atgtattcgc gcaatcctgg agagccactc tcagcctaat
    14881 ctgccagtat tacggccggc cggcaacgtc gagcggccgg attgtgcaat tcggctggat
    14941 ccaaaccgtt gaaatcggcc gagtgccgcc caatctatgc ggcgataact gaaaccagga
    15001 aacagccatg cgctttgaca aactgacaac ccggttccaa caggcgctgg ccgacgccca
    15061 gagcaaggcg gtcggccagg acaatccgta catcgagcct cagcatctgc tgctagcgct
    15121 gctggagcag gatgacggcg gtacggcgtc attgctcgcc cgcgccgggg tcaatgtgcc
    15181 acccttgaag gaggccctca aggccagcat cggccggcta ccgaaggtgg aaggccaggg
    15241 cggcgagatc agcgtttcgc gcgaactgac cagcctgttg aatctcaccg acaaggaagc
    15301 gcagaagcgt ggcgaccagt tcatcgcctc cgagctgttt cttctggcgc tggcggacga
    15361 caagggcgaa accggcaaac tgctaaaaag ccacggactc aatcgtaagg cgctcgaaca
    15421 ggccatcgag gcggtgcgcg gcggcgaggc cgtgcagagc caggaggcag aagggagccg
    15481 cgaagcactc aagaaataca ccctggacct gaccgagcgc gcccgcatcg gcaaactaga
    15541 cccggtgatc ggtcgcgacg acgagattcg cagagtgatc cagattctcc agcgccgcac
    15601 gaagaacaac ccggtgctga tcggtgagcc aggagtggga aagaccgcga tcgtggaagg
    15661 cctggctcag cgcatcatca acgaagaagt gccagagacc ctgaaaggca agcgcgtcct
    15721 cagcctcgac atggcggcgc tgttggcggg aaccaagtac cggggagaat tcgaggaacg
    15781 gctcaaatcc gtgctcaagg aaatctcgca ggaagaaggc cgcgtcatcg tattcatcga
    15841 cgaactccac acgatggttg gcgcgggcaa ggccgagggc gccatggacg cgggcaacat
    15901 gctcaaaccc gcgctggcgc gtggcgaact gcattgtgtc ggcgcgacaa cgctggacga
    15961 ataccgcaaa tacatcgaaa aggacgcggc actcgaacgg cgcttccaga aagtgctggt
    16021 cgatgagccc agcgttgaaa gcaccattgc catcctgcgc ggattgcagg agaagtacga
    16081 gttgcatcat ggcgtggaaa tcaccgatcc cgccattgtt gccgcagcgg agctttcgca
    16141 tcgttacatc accgaccgct tcctgcccga caaggccatc gacctgatcg acgaggcggc
    16201 tgcacgcatc aagatggaaa tcgattccaa gccggaacag atggataaac tcgaccgccg
    16261 gatcatccag ttgaagatcg agcgggaggc ggtaaagaag gaaaaggacg aagcctcgcg
    16321 caaacgcctc aaggcactcg aggaggagat caagcggctg gagaaggaat actcggatct
    16381 ggaagaaatc tggaaagcag aaaaagccca ggttcagggt tcgcagcaca tcaaggaaga
    16441 attggagaag ctgaagctgg aaatggaagc tgccaaacgc aagggcgatt ggcagaaggt
    16501 ctccgagctc caatacggca agatcccgca actggaatcg cagctcaaga aagcggaaac
    16561 cccggccgat cagccgaagt tcaggcttct gcgcactcag gtgggtgccg aagaaatcgc
    16621 cgaagtggtt tcgcgcgcca ccgggatacc ggtatccaag atgatgcagg gtgagcgcga
    16681 gaagctgatc aagatggaag agcacctgca tcagcgcgtg gtgggtcagg acgaagcggt
    16741 acggctcgtc gccgacgcga tccggcgctc gcgtgccggc ttggcagacc caaaccggcc
    16801 gtacggttct tttctgttcc tgggaccgac gggcgtcggc aagaccgaac tgtgcaaggc
    16861 gctggctgag ttccttttcg attcgcagga tcacctgatc cgtatcgaca tgtccgagtt
    16921 catggaaaaa cattcggtgg cccgcctgat cggcgcgccg cccggctacg tcggttacga
    16981 agagggcggg tatctgaccg aggcggtacg ccgcaagccc tattcggtga tcctgctcga
    17041 tgaggtcgag aaggcgcatc cggacgtgtt caacgtcctg ttgcaggtgc tggacgacgg
    17101 ccgcatgacc gacggtcagg gccgcacagt ggatttcaag aacacggtga tcgtgatgac
    17161 ttcgaatctg ggcagccaga tgatccagca gatggctggc gacgactatc agttgatcaa
    17221 gctggcggtg atgggcgagg tcaagtccta tttccggccg gaattcatca accgtatcga
    17281 cgaggtggtg gttttccacg cgcttgacga gaagcacatc aaatcgattg cgaagataca
    17341 gctcggccat ctggagaagc gcctggctgc gatggacctg aaactggaag tggacgatgg
    17401 cgtattggcc gaactggccc atgtcggctt cgatccggtg ttcggtgcgc gtccgttgaa
    17461 gcgcgccatc caggcgacga tcgagaatcc gctggctaag gaaatcctcg aaggacggtt
    17521 cgctcccaag gatgtgattc gcgtaacttc ggcgggaggc aagcttcgct tcgaaaaggg
    17581 aaaacaagcg gcccatgcag taggcgggag ctgaaaattg gtcggcggct ggatagagcc
    17641 gcgcttttga ccgataattc gtgccttgaa acgtgccggt cctctgcgaa agtgagcggt
    17701 aaggttgcgc tttggcctcc gagttgcaga gaattccggc ggcatctcac catacaagaa
    17761 catgaccgtc gaatcccccg ccgagccgct tgccttattc cgcggcgaac cggtgcacga
    17821 gctgcccggc gatctctaca tcccgccgga cgcgctgcgg gtttttctcg agaccttcga
    17881 gggtccgctt gacctgctcc tctacctgat ccgcaagcac aacctcgatg tgctcgacat
    17941 ccctatggcg agcctgaccc gccagtacat ggagtacgtc gaagtgatgc ggcagcatca
    18001 gctcgaactg gccgccgagt atctgctgat ggctgcactg ttgatagaga tcaagtcgcg
    18061 catgctgctg ccgcgaccga ctcaagtgag cccggaggaa gaggatccgc gcgcagaact
    18121 gatgcgccgg ttgctggaat acgagcgcat gaaactcgcc ggccaccagc tcaacgagtt
    18181 gcccctggcc ggacgggatt ttgctctggt gcaggtctgg ctcgaccggg aggtcgagca
    18241 gagactgccg gacgtcaatc ccgaagactt acgcaacgca tggctgggac tgatcgcgcg
    18301 cgcgcgcgtg acgcgccacc acaggatcac ccgcgaacag ctgtcggtgc gcgaacatat
    18361 gagccgcata ttgcgtagcc tgcagcacct gcgattcgtc cgcttcgagg accttttcga
    18421 tgaaacggca ggcgtacccg tcctggtagt cagttttctc gccatactcg aattggcccg
    18481 cgaaagcctc gtacaggtca cccagcaggg tgcgttttca cctatttatg tgaagttgaa
    18541 tcatggaatt acctctgtct gacccggccc agatcaagcg cgtgctggaa gcagcattgt
    18601 tgagtagtgc cgaacccttg cccgtggccg atctcagaaa gatgtttgac gacgacgtcg
    18661 gtcccgatgt cattcgccgc atgctggaag aactgcgcat ggaatggggc gagaaaggcg
    18721 cggaactggt gaatgtcgcc agcggatggc gttttcaggt ccggcccggc tatcagaagt
    18781 tcctggaccg gctgaatccg ctaaaaccgc cgaagtattc gagggcggtg ctggagacgc
    18841 tcgctatcat cgcctaccgg cagccggtca cgcgcggaga tatcgaggaa attcggggag
    18901 ttacggtatc cagcggcata ctcaaggcgc tcgaggcgcg cggctggatc gacgaggtcg
    18961 gtcaccgcga agtacccggc cggccggtgc tttactccac gacgcggacg tttctggacg
    19021 atttgaatgt gcgctcgctg gatgaactac cgccgctgga ggatctcgtc ccgctgatcg
    19081 aggcggaaca gccggctgtg ccggcgcaac atctcgaggt atccggagga ggtgcagccg
    19141 gatcggctca gaacgaggag gagaagtcct cagagacaca agccaatccg gcttgaatag
    19201 ccgctgcccg gcagcggtca atgccggtgc cgtactggag aacaacatga gagccgttct
    19261 tgttgccaat cccaaaggcg gatccggcaa gactacagtt gcgaccaacc tcgcggggtc
    19321 cctggcgacg cgaggggaga aagtctatct ctgggatctc gatcgccagc agtcttcgcg
    19381 caactggctg acgatccgtc cggcagaccg gccggcgatt caccggctgg acgcgcgaga
    19441 tgaccacgag ccttccatgc cgcgcggctc gagctggctc gttctggact ccgcagccgg
    19501 gttgcatggc aagaacctcg cgcacgcact gaaactcgcc cagaaagtgc tggttccggt
    19561 gcagccatcg gttttcgaca tggcggccac gagtactttc ctgcaaacct tgatggaaga
    19621 gaaggcgata cgaaagcaca agacctttgt aggcatcgtt ggcatgcggg tggatccgag
    19681 aacaaaggcc gcggcgactc ttgaagcctt tctagcccaa ttcaaattgc ccgtgttgac
    19741 ctatcttcgc gacacgcagg tctatgtgaa cgcggctttc aatggtctca gcgtgttcga
    19801 cttgccagat tacctgagcg cccgagacat agagcagtgg caggcgttac tggcctggtt
    19861 ggaagaggac tgaaaagccg gtacctcgcg accggacccc gctctttggt tgacgcacat
    19921 ctcgttacat tgctatcatc ccgcgtcttg gaactcgatt tttcgggttt caatccggtc
    19981 gcggggtgga gcagtctggc agctcgtcgg gctcataacc cgaaggtcgt aggttcaaat
    20041 cctgcccccg caaccaacct gctctttaaa aaatcaaaca gccgatgcgt gtgggtgctt
    20101 cgtgtggtgg ggcgcttgcg cggggtgggt gggtcatcgc ccggttctta ggtcttgaag
    20161 aagacgttag gacaagccgc tctgcgaaga agctttggtc tatgaagtgc tcacgcgtaa
    20221 gtaatttgta gttgtgaatt gctttgagtg agttgctggt cccgattggg gattggcaca
    20281 aacgaggatt gaactgaaga gtttgatcct ggctcagatt gaacgctggc ggaatgcttt
    20341 acac
//
LOCUS       DASQOP010000140         3585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1669442, whole genome shotgun sequence.
ACCESSION   DASQOP010000140 DASQOP010000000
VERSION     DASQOP010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1669442"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            268..1794
                     /locus_tag="VE030_05725"
     CDS             268..1794
                     /locus_tag="VE030_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HZF22949.1"
                     /translation="MTVIRQEDLIESVADALQYISYYHPMDYIKSLADAYRREESPAA
                     RDAIAQILTNSRMCAEGKRPICQDTGIVVAFLRVGMEVRWDGASMSVSDMVNEGVRRA
                     YLHPDNKLRASILADPAGTRKNTRDNTPAVIHMELVPGDTVDVRIAAKGGGSENKAKF
                     VMLNPSDSIVDWVLKTVPAMGAGWCPPGMLGLGVGGSAEKAMVLAKEALMQPIDMHEL
                     KQRGPKSRIEELRIEIFDKVNALGIGAQGLGGLTTVLDVKILDYPTHAASLPVAVIPN
                     CAATRHAHFTLDGSGPARLDPPSLDDWPDVKWAPAPASRKVDLSKVTREEIVAWKPGE
                     TLLLNGRILTGRDAAHKRIQELFARGETLPAGVDFRNRFIYYVGPVDPVRDEVVGPAG
                     PTTATRMDKFTDMMLAKTGLLGMIGKGERGPAAIEAIRKHKAVYLMAVGGAAYLVSKA
                     IKSSRVVAFADLGMEAIYEFEVKDMPVTVAVDVNGTSVHQTGPREWQARIGKIPVSVR
                     "
     gene            1896..1993
                     /gene="ffs"
                     /locus_tag="VE030_05730"
     ncRNA           1896..1993
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VE030_05730"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            2098..>3585
                     /gene="dnaX"
                     /locus_tag="VE030_05735"
     CDS             2098..>3585
                     /gene="dnaX"
                     /locus_tag="VE030_05735"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HZF22950.1"
                     /translation="MTLALARKWRPRNFAELIGQDHVVRAISNALTQNRLHHAYLLTG
                     TRGVGKTTLARIIAKALNCEIGVSADPCGKCGACTEIDAGRHVDVIELDAASNTQVDN
                     MRDLLENALYAPTSGRFKVYIIDEVHMLSRNAFNAMLKTLEEPPAHIKFILATTDPQK
                     VPVTVLSRCLQFNLKQIPPGLIRGRLEYVLGKEGVPFETPALNLLARAAEGSMRDALS
                     LLDQAIAHGAGKVEETSVREMLGAVDRSYLFSLLKALASGQGPALLAEAERMAARSLS
                     FDAALQELATLLHRVALAQTLPEALADDEPERDTLLGLAGAFSEEDAQLLYQIAIHGR
                     RDLGLAPDEFAGFTMTLMRMLAFVPVEPGESALAATPGTGRPASKAAASAGTGAATGT
                     QQATGKASVNAERELADWRTLVGRIKIGGMARMLADNCEFRSLEGDRLELAVPEPHKH
                     LLEKVYTDKLQAALAEYFGRKLRLRISTGGTGNTPAEIENQERQAK"
BASE COUNT          714 a         1115 c         1154 g          602 t
ORIGIN      
        1 gataagccgc cattccggag acgttggcct gcttgacgaa gtccgcaggc ggcgggaaaa
       61 cgcggtgttc ctgcaggacc gattcgatcg tactcattgc atcctcccca tcgtatttcc
      121 tgatagtcgg ctcttcaacg gattatagcc agaacttccg gtatccagtc ctgcgtgacg
      181 cgcatcgggg cggcaggcaa gcgttgccgg tagtgtaaaa tccgaatgag cgcataagca
      241 attcgccgag ccgccccgga ttcatccatg accgtcatcc gccaggaaga cctgatcgaa
      301 agcgtcgccg acgccctgca gtacatctct tactatcatc cgatggacta catcaagtcc
      361 ctcgcggatg cctataggcg tgaagagtcg ccggcggccc gcgatgcgat cgcgcaaatc
      421 ctcaccaact cgcgcatgtg cgcggaggga aagcggccga tctgccagga caccggaatc
      481 gtggtcgcat ttttgcgcgt cggcatggag gtgcgctggg acggtgccag catgagtgtt
      541 tccgacatgg tcaacgaagg cgtgcgccgc gcctacctgc acccggacaa caagctgcgc
      601 gcgtccatcc tcgccgatcc ggcgggcacc cggaaaaaca cgcgcgacaa cacgcctgcg
      661 gtgattcaca tggaactcgt tccgggcgat accgtggatg tccggatcgc ggccaagggt
      721 ggcggttccg agaacaaagc gaaattcgtc atgctcaacc cgtccgacag catcgtggac
      781 tgggtattga agaccgtgcc ggccatgggc gcaggatggt gcccgccggg catgctcggg
      841 ctgggtgtcg gcggcagcgc ggagaaggcg atggtgctgg ccaaggaagc gctgatgcag
      901 ccgatcgaca tgcacgaact gaaacagcgc gggccgaaaa gccgcatcga ggaactgcgc
      961 atcgagatct tcgacaaggt caatgcgctc ggcatcggcg cgcagggcct cggcgggctg
     1021 accacggtgc tcgacgtcaa gattctcgat tacccgacgc acgcggcatc gctgccggtg
     1081 gcggtgattc ccaattgcgc tgctacccgg cacgcgcact tcacgctcga cggcagcggt
     1141 ccggcgcggc tggatccgcc cagcctcgat gactggccgg acgttaaatg ggcgccggcg
     1201 ccggcctcgc gcaaggtcga cctttccaaa gtcacacgcg aggagatcgt ggcctggaag
     1261 cccggcgaga ccctgctgct gaacggccgg atccttaccg gacgcgatgc cgcgcacaag
     1321 cgcatccagg aactgttcgc caggggcgaa acgctgcccg caggcgtgga cttccgcaac
     1381 cgcttcattt actacgtcgg tccggtggac ccggtgcgcg atgaagtcgt cggcccggcc
     1441 ggtccgacga ccgccacccg catggacaag ttcaccgata tgatgctggc gaaaaccggg
     1501 ctgctcggca tgatcggcaa aggcgagcgc ggccccgcgg cgatcgaagc cattcgcaag
     1561 cacaaggcgg tgtacctgat ggcggtgggt ggggcagctt atcttgtctc caaggccatc
     1621 aaatcgtcgc gcgtggtcgc gttcgcggat ctcggcatgg aagcgatcta cgagttcgag
     1681 gtgaaggata tgccggtcac cgtcgcagtg gacgtcaacg gcacttcggt gcaccagacc
     1741 ggtccgcgcg agtggcaggc gagaatcggc aagattccgg tgtcggtccg gtgacggaca
     1801 cgaggcgaga ggctgtaggc aagaggctag aaggaatcct attggatttc ctctcacctc
     1861 acgcctctcg cctcgcgcct tacgaaagtt tcaatggcgg gcctccccgc atcgcatggt
     1921 agtgaacctg gtcaggtccg gaaggaagca gccacagcta ttctttgcga gtgacggggt
     1981 aaggctcgcc acccctatga aacgtgaggc ggcaagcgtg aggcatgaac ggtttggtca
     2041 ctcgaacagc tttgccgcag gaataattca ccgataaatt tgcggtccaa aaacaaagtg
     2101 acgcttgcac tcgcgcgcaa atggcgcccc aggaatttcg ctgaactgat cgggcaggac
     2161 catgtggtcc gcgccatcag caacgcgctc acgcagaatc gcctgcatca cgcgtatctg
     2221 ctgaccggca cccgcggtgt gggcaaaacc acgctggcgc ggatcattgc aaaggctctg
     2281 aattgcgaaa tcggcgtttc cgcggatccc tgcggcaagt gcggcgcctg caccgagatc
     2341 gacgccggac gccacgtgga cgtgatcgaa ctcgacgccg cctccaacac ccaggtggac
     2401 aacatgcgcg atctgctcga gaacgcgctc tatgcgccga cttccggccg cttcaaggtg
     2461 tacatcatcg acgaagtgca catgctgtcg cgcaacgcct tcaacgcgat gctcaagacc
     2521 ctcgaagagc cgccggccca tatcaagttc atacttgcga ccaccgatcc gcagaaagtc
     2581 ccggtgacgg tgttgtcgcg ctgcctgcaa ttcaatctca agcagattcc cccggggctg
     2641 atccgcggcc ggctcgaata cgtgctcggc aaggaaggcg tgcctttcga gacgccggcg
     2701 ctcaatctgc tggcgcgcgc cgccgagggc agcatgcgcg atgcattgtc gctgctggac
     2761 caggcgatcg cgcacggcgc cggcaaggtc gaagagacct cggtcaggga gatgctgggc
     2821 gcggtggacc gcagctacct gttcagcctg ctcaaggcgc tcgcgagcgg ccagggtccg
     2881 gcattgctgg ccgaggccga gcgcatggcg gcgaggagcc tgtcgttcga cgcggcgctg
     2941 caggaactgg cgaccctgct gcaccgggtt gctttggctc aaaccttgcc ggaagcgctg
     3001 gcggacgacg agcccgagcg cgatacgttg ctcggactgg ccggcgcctt cagcgaagag
     3061 gatgcacaac tgttgtacca gatcgcgatc cacggacggc gcgatctggg gctcgccccg
     3121 gatgaatttg ccggcttcac catgacgctg atgcgcatgc tcgcattcgt gccggtcgag
     3181 ccaggagagt ccgcgttggc cgccacgcct ggcactggcc gtcccgcgtc aaaggccgca
     3241 gcatctgccg gaactggggc cgcgacgggc acacagcaag cgactggcaa agcttccgtc
     3301 aacgcggagc gggaactcgc cgattggcgc acgctggtcg ggcgcatcaa gatcggcggc
     3361 atggcacgca tgctggcgga caactgcgag tttcgatcgc tcgaaggcga ccggctggaa
     3421 ctcgcggtgc cggagccgca caaacatctg ctggaaaagg tgtacaccga caagttgcag
     3481 gcggcgctcg cggagtattt cggtcgtaag ttgcggcttc ggatctcgac cggcggcacc
     3541 ggcaatacgc ccgccgaaat cgagaaccag gaacgccagg cgaaa
//
LOCUS       DASQOP010000141         5022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1466946, whole genome shotgun sequence.
ACCESSION   DASQOP010000141 DASQOP010000000
VERSION     DASQOP010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5022
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1466946"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..753
                     /locus_tag="VE030_05740"
     CDS             4..753
                     /locus_tag="VE030_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZF22951.1"
                     /translation="MVGFIVGMTGVGGGSLMTPLLVLVFGINPATAVGTDLLYASLTK
                     SAGVWVHSKRGNVDWKLVGWLAAGSLPAALLCVVLLRVLGIETSRVSGIITGSLGVAL
                     ILTALSLLFKERLQRLASRKVLEGESWRQRHVAAVTVATGAVLGALVTLSSVGAGALG
                     VTALLFLFPLLPAARIVGSDIAHAVPLTAVAGLGHLSMGTVDLGLLGSLLVGSLPGIY
                     LGSHASSRVPERFLRPALASLLVIIGGKLVW"
     gene            790..2505
                     /locus_tag="VE030_05745"
     CDS             790..2505
                     /locus_tag="VE030_05745"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HZF22952.1"
                     /translation="MYVYDQVDRNLVAERVAQYRDQVRRFLAGDLTEDEFRPLRLQNG
                     LYIQKYAPMLRVAVPYGLLSAAQLRMLAYIARRYDRGYGHFSTRQNIQYNWPRLEDSP
                     DILADLASVEMHAIQTSGNCVRNITSDEFAGVAPDEIVDPRPLCEILRQWSTFHPEFS
                     FLPRKFKIAVNASVEDRSATAFHDIGLYLRRAPDGELGLRVLVGGGMGRTPVVGTVIR
                     EFLPWQHMMTYVECILRVYNRYGRRDNIYKARIKILVKALGAEEFARQVEEEWALVKD
                     GPGTLTGEEFNRVSAYFVAPDYHPRPEDEGQLARRIAESRGFARWAGRAVRPHRVPGY
                     RAVVLSLKKTGVAPGDATAEQMDRVADWSERFGFGEIRVSHEQNLILPDVRAGDLYTL
                     WEEARQAGLATPNLGLLTDIIACPGGDFCSLANAKSIPIAEAIQRRFDDLDYLHDIGE
                     LNLNISGCMNSCGHHHAGHIGILGVDKDGEEWYQVSIGGADGSDAGHGRSAIGKVIGP
                     SFKAEDMPDVVSRLVEIYLEKREADERFVDTVERLGIAPFKAGVYGTPARTKKEPSEG
                     VVTDG"
     gene            2498..3031
                     /locus_tag="VE030_05750"
     CDS             2498..3031
                     /locus_tag="VE030_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HZF22953.1"
                     /translation="MAEIIRGGAVVADDWQILRPAPETPLAGVEIPAGRFVVPLALWL
                     ERHAELIARGDVGAWLAGADDPSPIAPWLPQLPLVAVDFPKFTDGRGYSVAYLLRSRF
                     GYRGELRAIGDVLPDQLFFMRRVGFDAFAVRPDKDIRQALRSLQPFSDAYQGSWDNPV
                     PAFRRHVRAWPAPPQGN"
     gene            3047..3784
                     /locus_tag="VE030_05755"
     CDS             3047..3784
                     /locus_tag="VE030_05755"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HZF22954.1"
                     /translation="MNAPSPAIAAAALYPYQTLTARLEAIAAEFPHAAFASSLGAEDM
                     VVTDAILSGGLPIAIFTLETGRLPRETLDLLERVRSHYGHEIEAFQPDPDAVARYVKE
                     HGLNAFYESVDLRERCCHIRKVEPLARALAGRDAWITGLRRAQSVTRNELPLRERDTV
                     HGIPKFNPLADWSGAEVWACIRERGVPYNALHDRGYPSIGCEPCTRAIRPGEDIRAGR
                     WWWESSDAKECGLHVSPLGKPSEGRTS"
     gene            3781..4713
                     /gene="cysD"
                     /locus_tag="VE030_05760"
     CDS             3781..4713
                     /gene="cysD"
                     /locus_tag="VE030_05760"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HZF22955.1"
                     /translation="MSAVLERIEESSSRIDHLDWLESEAIHILREVAAECRNPALLFS
                     GGKDSICLLRLAEKAFRPGKFPFPLLHIDTGHNFPEVIAFRDARAAELGERLVVGHVE
                     ESMRKGRVRLRSEDESRNKHQSVTLLDTIAEHGFDACIGGARRDEEKARAKERIFSFR
                     DEFGQWDPKNQRPELWSLFNTRVHPGENIRVFPISNWTELDVWQYIQREKLAVPSIYY
                     AHRREVIRKPGGLLPLSELVQPKPGEAVETLSVRFRTVGDMTCTCPVESTAVNAEDII
                     AETAITRITERGATRMDDQTSEASMELRKKEGYF"
BASE COUNT          879 a         1567 c         1649 g          927 t
ORIGIN      
        1 ttcgtggtcg ggttcatcgt cggcatgacc ggcgtgggcg gcggttcgtt gatgaccccg
       61 ctgctggtcc tggtattcgg catcaatccg gcgaccgcgg tgggcaccga tcttctgtat
      121 gcctcgctta ccaagagcgc cggcgtctgg gtgcattcga agcgcggtaa cgtcgactgg
      181 aaactggtgg gctggcttgc ggcggggagt ctgcccgccg cattgctttg cgtagtgttg
      241 ttgcgggtgt tgggcatcga gacctcgcgc gtgtctggaa tcatcaccgg ctcgcttggc
      301 gtggcgctga ttctgactgc gctttcgctg ctgttcaagg agcgcttgca gcggctggcc
      361 tcgcgcaaag tcctcgaggg cgagagctgg cgccagcgtc atgtggccgc agtgacggtt
      421 gccaccggtg cggtgctggg cgctctggtc acgctgagtt cggtgggggc gggtgcgttg
      481 ggcgtgaccg cgctgttgtt cctgttcccg ttgctgccgg cggctcgcat cgtcggcagc
      541 gatattgccc acgctgttcc gctaactgcg gtggcggggt tgggtcacct gtcgatgggc
      601 acggtggacc tcggcctgct cggcagcctg ctggtcggtt cgctgcccgg aatctacctc
      661 ggcagtcacg caagttcgag ggttccggaa cggttcctga gacccgcgct ggcatcgctg
      721 ctggtaatca tcggcggcaa gctggtgtgg tgacgcggca gtggttgttt cacgaaacga
      781 aggatgtcga tgtacgtata cgatcaagtt gaccgcaatc tcgtcgccga gcgcgtggcg
      841 cagtaccgcg accaggtgcg gcgcttcctc gcaggggatc tgaccgagga cgagttccgg
      901 ccgctgcgcc tgcagaacgg gctgtacata cagaaatatg cgcccatgct gcgcgtggcg
      961 gttccctacg gccttctgtc ggctgcgcag ctgcgcatgc tggcgtacat cgcgcgccgt
     1021 tacgaccgcg gctacgggca tttctccacg cgccagaaca tccagtacaa ctggccgcgc
     1081 ctcgaggatt cacccgacat cctcgccgac ctcgcgtcgg tggagatgca tgccatccag
     1141 accagcggca actgcgtgcg caatatcacc tcggacgagt tcgccggcgt tgcgcccgac
     1201 gagatcgtcg atccgcgccc gctgtgcgag atcctgcgcc agtggtccac cttccacccg
     1261 gaattcagtt tcctgccgcg caagttcaag atcgcggtca acgcttccgt ggaagatcgt
     1321 tccgccaccg ccttccacga catcggcctg tacctgcggc gcgcgccgga cggcgaactc
     1381 gggctgcgcg ttctggtggg gggcggcatg ggccgcacgc cggtcgtggg cacggtgatc
     1441 cgcgagttcc tgccctggca gcacatgatg acttacgtcg agtgcatcct gcgcgtctac
     1501 aaccgctacg ggcgccgcga caacatctac aaggcgcgca tcaagatcct ggtgaaggct
     1561 ctcggtgccg aagagttcgc gcgtcaggtg gaggaagagt gggctctggt gaaggatggc
     1621 ccgggcacgc tcaccggaga ggaattcaac cgcgtctccg catatttcgt cgcgccggac
     1681 taccacccgc ggccggagga tgaaggccaa ctggcacgcc ggatcgccga gagccgcggc
     1741 ttcgcccgtt gggcggggcg ggcagtgcgc ccgcaccgtg ttccgggcta ccgggcggtg
     1801 gtcctgtcgc tgaaaaagac cggcgtggcg ccgggcgatg cgactgcgga gcagatggat
     1861 cgggtggccg actggtccga acgcttcggt tttggcgaaa tccgcgtttc gcacgaacag
     1921 aacctgatcc tgcccgatgt gcgcgccggt gacttgtata cgctttggga agaggcgcgg
     1981 caggccgggc tggccacccc gaacctcggg ctgctcaccg atatcatcgc ctgccccggc
     2041 ggcgacttct gctcgcttgc caacgccaag tcgatcccga ttgccgaggc gatccagcgc
     2101 cgcttcgacg acctggacta tctccacgac atcggcgagt tgaacctcaa catttccggc
     2161 tgcatgaact cgtgcggcca ccatcacgcc ggccacatcg gcatcctcgg cgtcgacaag
     2221 gatggcgagg agtggtacca ggtgtcgatc ggcggcgccg acggttccga cgccgggcac
     2281 ggccgcagcg cgatcggcaa ggtaatcggg ccgtcgttca aggccgaaga catgcccgac
     2341 gtggtatcgc gtctggtgga gatctacctg gaaaagcgcg aagccgacga acgtttcgtg
     2401 gacaccgtgg agcggctggg catcgccccg ttcaaggccg gggtgtacgg gactccggcg
     2461 cgcacgaaaa aagaaccgag tgaaggagtg gtcaccgatg gctgaaatca ttcgcggcgg
     2521 cgccgtcgtt gcggacgact ggcagattct gcgcccggcg ccggaaacgc ctctggccgg
     2581 ggtcgagatc cccgcaggcc gtttcgtagt tccgctcgcc ctgtggctgg aacggcacgc
     2641 cgagctgatc gcgcgtggcg acgtcggcgc gtggctcgcc ggcgccgacg atccgtcacc
     2701 gatcgcgccg tggctgccgc agttgcccct ggtggcggtg gactttccga agtttaccga
     2761 cgggcgcggc tattccgtgg cttacctgct gcgctcgcgg ttcggctatc ggggtgaatt
     2821 gcgcgcaatc ggtgacgtgc tgcccgacca gctttttttc atgcgtcgag tgggcttcga
     2881 tgcgtttgcc gtgcgcccgg acaaagacat ccgccaggcg ctgcgttccc tgcaaccgtt
     2941 ctcggacgcc taccagggct cgtgggacaa cccggtgccc gcattccgcc gccacgtgcg
     3001 cgcctggccg gcgccgccac agggcaacta aaaagagacc gcggaaatga acgcaccatc
     3061 tcccgccatc gcagcggctg ccctctaccc gtaccagact ctgaccgcac ggctcgaagc
     3121 catcgctgcc gaatttcccc atgccgcctt tgccagcagt ctgggtgcgg aagacatggt
     3181 cgtgaccgat gcgatcctga gcggcggtct gccgatcgcg atcttcaccc ttgaaaccgg
     3241 acgcctgcct cgcgaaacgc tggatctgct ggaacgcgtg cgcagccact acggccatga
     3301 gatcgaagcg ttccagccgg accccgatgc ggtggcacgc tacgtgaagg aacacggctt
     3361 gaacgctttt tacgagagcg tcgatctgcg cgagcgttgc tgccatatcc gtaaagtcga
     3421 gccgctggca cgggcgcttg ccggcaggga cgcctggatc acgggactgc gccgtgcgca
     3481 atcggtgacc cggaacgaac tgccgctgcg cgagcgcgac acggtgcacg gcatcccgaa
     3541 attcaacccg cttgccgact ggagcggggc cgaggtctgg gcctgcatca gagagcgtgg
     3601 tgtgccgtac aacgcgctgc acgatcgcgg ctacccctcg atcggctgcg aaccctgtac
     3661 ccgcgcaatc cggcccggcg aggacatccg cgccggacgc tggtggtggg aaagttccga
     3721 cgcgaaagag tgcgggctgc atgtttcacc cttgggcaaa ccgagcgaag gaaggacatc
     3781 gtgagtgccg tgttggaaag aatagaagaa tcgtcgtcgc gtatcgatca tctggactgg
     3841 ctcgaatccg aggccatcca catcctgcgc gaggtggcgg ccgagtgtcg caacccggca
     3901 ttgctgtttt ccggtggcaa ggattcgatc tgcctgctgc ggctggcgga gaaggcattc
     3961 cgtcccggga agtttccgtt tccgttgctg cacatcgata ccgggcacaa cttccccgaa
     4021 gtgatcgcct tccgcgacgc gcgcgcggcg gaactgggcg agaggctggt cgtcggccac
     4081 gtcgaggaat ccatgcgcaa gggcagggtg cggctgcgct ccgaagacga gagccgaaac
     4141 aagcatcagt cggtgacgtt gctcgacacc attgccgagc acggtttcga tgcctgcatc
     4201 ggcggagcgc gccgcgacga ggagaaggcg cgcgcaaaag aacgcatctt ctcgttccgc
     4261 gacgagttcg gccagtggga cccgaaaaat cagcggccgg aactatggag cctgttcaac
     4321 acccgcgttc acccgggcga gaacatccgc gttttcccga tcagcaactg gaccgaactg
     4381 gacgtctggc aatacatcca gcgcgaaaag cttgccgtgc cgtcgatcta ctacgcgcac
     4441 cggcgcgaag tcatccgcaa gcccggcggc ctgttgccgt tgtccgaact ggtgcaaccc
     4501 aaacccggcg aggcggtgga gacgctttcg gtgcgtttcc gcaccgtcgg cgacatgacc
     4561 tgcacctgcc cggtcgagtc caccgccgta aacgccgaag acatcatcgc cgagacggcg
     4621 atcacccgga tcaccgaacg gggcgcaacg cgcatggacg accagacctc ggaagcgtcg
     4681 atggaactgc gcaagaagga aggctatttc taaagactag ctttcaccgc aaagacgcaa
     4741 aggactactg gaaaattatg aagacgagag actggaaatt ccgtggttgc cacagcgccg
     4801 ggaccaatat gcgttttcca atacagcttt ctttgcgttc ctttgcgtct ttgcggtgag
     4861 aatcttggtg gttaaatgtc tgcaatagag aatattcagc ccgttgacac cggcatgctg
     4921 cgcttcacca gcgcgggcag cgtggatgac ggcaagagca cgctgatcgg gcggctactg
     4981 ttcgatacca aggcaatttt cgaggatcag ctttcttcga tc
//
LOCUS       DASQOP010000142        32607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_461056, whole genome shotgun sequence.
ACCESSION   DASQOP010000142 DASQOP010000000
VERSION     DASQOP010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 32607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32607
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461056"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..1933)
                     /locus_tag="VE030_05765"
     CDS             complement(35..1933)
                     /locus_tag="VE030_05765"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZF22956.1"
                     /translation="MSDLRPVQLLCRVGMDIAAWFCMPAVFLFVYVNHYSESADAVLP
                     HVRMMALVLLGLVLVRLLLSFLIPSRALSRFAVSIVTSALLATIFSYYALVLIGLQSW
                     GRVISWDLITSYSSQVLELADALEISATSAIAGFVLAYLGLLGVTWTYFYYFDWAPFV
                     STTGPKWLLALFLVSGCMVLAIERYSFLAAPWVLDLEPISLTFFPMEAARNLQGHAID
                     RLSSGNRDRMEESARASYKPMLKAGRRNLILIVVDALRPDHMGVYGYERDTTPNLSRL
                     MNAGMVRKALTVHASCSSSACGLLSLASSKFVHQFSNHPFILHEALKRQGYRVHMVLS
                     GDHTNFYALKRIYGDVDSYYDGMSARRFRYMNDDQLVLDRLGTFPVWDKEPVMIHFHL
                     MATHLLGKRHGITNKYLPAANYALPENQDSATGGRVSERIANYYDNAVLQADLEIHTI
                     LEILRGKGYLNDALVVVTADHGESLGEHGVFQHTNSVYEQVLRIPFLLISYGRQHGRP
                     INRRTASQVDIAPTIFAELGMPRPATWVGLPMQNASDGEFSYFQEQSEVGLFDHRDPR
                     NLWKYWINSRTGKEYAFNISLDPEESVSMTGSVVLKNLREWRLKVLPAASVAFDRPTP
                     ANGSAIAQ"
     gene            complement(1926..2546)
                     /locus_tag="VE030_05770"
     CDS             complement(1926..2546)
                     /locus_tag="VE030_05770"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZF22957.1"
                     /translation="MQKISQDNVIRAYRFYAPFYDYLYGSVLEPGRRAMSEAVDLLRP
                     ASLLEIGVGTGLMLARYPAYSKVVGIDISAEMLEIAWQRVRRLTGRDIRLEAMDAEET
                     DFPDGFFDCVTIPYALSVTPDPERLIAEARRVCRKNGTILILNHFSGSRFWWLLEQAL
                     QSIASRVGFRFDFSFADQILKYDWEIRSVKEVNFFGLSRLVEIRNV"
     gene            complement(2706..2782)
                     /locus_tag="VE030_05775"
     tRNA            complement(2706..2782)
                     /locus_tag="VE030_05775"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2746..2748),aa:Arg,seq:ccg)
     gene            2897..3445
                     /locus_tag="VE030_05780"
     CDS             2897..3445
                     /locus_tag="VE030_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZF22958.1"
                     /translation="MAEVHMEERSSPIKTPKQLVVAIVLAFLVPVLGIVMIAELATSG
                     LKVDPKSSAMSDEAVARRLKPVGEVVIGEAGAPVPATTTKSTAGGPTQIAAAAKSAGG
                     AADGKKTYDAVCAMCHAAGVAGAPKTGDKAAWKPRIAQGKDALYTSALKGKNLMPPKG
                     GAASLPDADIKAAVDYLVSQAK"
     gene            3579..4391
                     /locus_tag="VE030_05785"
     CDS             3579..4391
                     /locus_tag="VE030_05785"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HZF22959.1"
                     /translation="MSTYAIGDLQGCYDPLRELLEEIGFSATRDRLWFVGDLVNRGPQ
                     SQEVLRFVKGLGSRAVTVLGNHDLHLLMVAEGRAKLHKGDTLAAILEAPDREELLAWM
                     RGLQLMHVEGEYAMVHAGLLPSWSVPKALDLGHEAEEVLRGPGYRSLMAHMYGNQPDR
                     WDDALTGYDRLRVIINVMTRLRICTPDGRMEFSHKGRLEDIPEGYVPWFAAPNRKSAN
                     ATVICGHWSALGLLAEKNLLALDSGCLWGRRLSAVRLEDRRTFQVTCPKFAD"
     gene            complement(4366..5145)
                     /locus_tag="VE030_05790"
     CDS             complement(4366..5145)
                     /locus_tag="VE030_05790"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZF22960.1"
                     /translation="MALHDREQTSIPVRFYRATLLLFHLFAGIATVALLFPFYGNARR
                     RLAVARWSARILAAVNVEPRLRGVPPEVRDRAAVLVANHVSWLDIPLVHSVWQVRFIA
                     KSEVRRWPLIGWLSARTGTLFIRRGKSRHAARINEAIHAAFLAGDAIGVFPEGTTSDG
                     TGLQPFHASLLQPAVDERALVFPVALRYLDEKRRVNVNASYVGDTSLIESMAMILRER
                     KIVAEMIFLPAIDAEGKSRRDLAREAHAAITAAFSRRTSGT"
     gene            complement(5190..6125)
                     /locus_tag="VE030_05795"
     CDS             complement(5190..6125)
                     /locus_tag="VE030_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HZF22961.1"
                     /translation="MRTLICGSIAYDTIMVFGDYFKNHILPDQIHILNVAFLVPDLRR
                     EFGGCAGNIAYTLKMLGGDPLIMATVGEDYQPYAYRLEKLRLSQAHVRQVAGTFTAQA
                     FITTDLDDNQITAFHPGAMNSSHLNRVADARDVGLGIVAPDGREGMLQHAREFHQAGV
                     PFIFDPGQGLPMFNSEELLNFIRQAEYVATNDYEAKLLQERTGRTVEELAKLVTAFVV
                     TQGAQGSLVYSGGGIERIPSVKPARVVDPTGCGDAYRAGLLYGIANDLDWPTTGRLAS
                     LLGSVKVAERGAQNHQFSRDEIGARFRDAFGRSPW"
     gene            complement(6147..7031)
                     /locus_tag="VE030_05800"
     CDS             complement(6147..7031)
                     /locus_tag="VE030_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HZF22962.1"
                     /translation="MPESFTRPSFLAPRPSAIVIAIFVAMMIAGCGSVPSRPGSKPGG
                     YYLDDGPDANPPADLDSMPEPAPKIEPINKYTSRPYTVLGRTYTPYTQLTAYKQRGVA
                     TWYGKRYHGQKTSSGEVYDMYALSAAHTLLPLPSYARVTNVANGKSVVVRVNDRGPFH
                     EDRLIDLSYAAAHRIGIIGQGSAVVEVEAIIPGSGEAPVVASVPAAPAPVAVPVSNEA
                     GGIFVQLGAFSVEQNADAFLRKMRMDLGWLAGSMQLYRGEGLYKVHAGPYPNRGEAER
                     AAERIRQELGFTPFVLKR"
     gene            complement(7124..8218)
                     /gene="rodA"
                     /locus_tag="VE030_05805"
     CDS             complement(7124..8218)
                     /gene="rodA"
                     /locus_tag="VE030_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HZF22963.1"
                     /translation="MINRLYAYLVKHLDRYLLTLVAILLVMGLLILYSASGTSLERVA
                     AQMGNICAALVVMWIAANVPLHYLSRIALPLYVLGLALLIAVAAFGEIVNGARRWLDL
                     GFTRIQPSELMKIAVPLMLAWYFDRYEAVLKMRDFAVGALLVLVPVLLIAKQPDLGTA
                     LLVTAAGFFVLFLAGLSWKILVGLALAGGASLPFLWSMLHDYQRQRILTLLDPSQDPL
                     GAGYHTIQSTIALGSGGFFGKGWLNGTQSHLDFLPERTTDFIFAVYAEEFGLFGNLVL
                     MVLFLLVVGRGLVIAANAPTLFTRLLAGGITLTFFTYAVVNMGMVSGMLPVVGVPLPL
                     ISYGGTSLVTILFSVGLLMSIHTHRRLVQT"
     gene            complement(8211..10199)
                     /gene="mrdA"
                     /locus_tag="VE030_05810"
     CDS             complement(8211..10199)
                     /gene="mrdA"
                     /locus_tag="VE030_05810"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HZF22964.1"
                     /translation="MNRAVELRDHQRELYNFQLRLAIAGGAVLFAFFVLFVRFFYVQV
                     IQHDYYHTLAEQNRIFIVPVVPNRGLIVDRNGVVLAHNFAAYTLEIMPTQVADFDDMI
                     NRLSILVDITPKDRKRFQKLLEEGHDFASVPIRTRLNDEEVARFAVNAYRFPGVELNA
                     RLFRHYPNGEIASHVVGYIGRISDPDVADLKNKGMAANYSASDYIGKVGLEQRYEKDL
                     RGVTGYEHVETDANGRSVRTLRSILPVSGNDLRLALDARLQQVAEQVFGTHRGSLVAI
                     EPATGGVLALVSRPGFDPNLFVEGIDPQNWEALNNSPDHPLNNRALQGVYPPGSTFKP
                     FMALAGLESGKRKPGFTISDPGYFVLGGGGSGRVYRDWKAGGHGIVNLHKAVVESCDT
                     YFYGLAHELGIDFIHEFIGQFGLGSTTGIDIEGEVGGLLPSQEWKQRRFQQKWFVGDT
                     ISVGIGQGYNLTTPLQLAFATAILANDGRIFRPHVVQHIQDGQTGELTSIEPEPIGEV
                     ALKPENVKRVRDAMVDVMKPGGTAAWAGMGAQYLFAGKTGTAQVIGLKGQKYDEDRVH
                     ARHRDHALFIGYAPADDPKIALAVLVENGGHGSSTAAPIARQVIDFYLLGKEPQPVKP
                     ATRKPVREPAQETVPELAPEQERALEHVLEPDPEREGD"
     gene            complement(10244..10774)
                     /gene="mreD"
                     /locus_tag="VE030_05815"
     CDS             complement(10244..10774)
                     /gene="mreD"
                     /locus_tag="VE030_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HZF22965.1"
                     /translation="MRGSGFSNLSTQEILLPVRPLYVVVTLAAALLLNLLPLAGWVLA
                     LRPDFVALTLLYWGINQPRKIGFLPAWLLGLAVDVAEGSLFGQHALAYAVMMFSAFAL
                     HRRVAMFGVRHQIPHVLVILLLMQVIVLAVRAAAGGEYPGWWYFVPSVVGALLWPVTR
                     FLFTIPLRSRRDPDEV"
     gene            complement(10774..11676)
                     /gene="mreC"
                     /locus_tag="VE030_05820"
     CDS             complement(10774..11676)
                     /gene="mreC"
                     /locus_tag="VE030_05820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HZF22966.1"
                     /translation="MEYTPPPFFKRGPSLIARLSFFVLLSLVLLYADARFHYMEGLRK
                     AIGVVLYPLQRIVDVPAEVASGIGSFFVSQSYLQRENERLVRENFLNAGLLQTQQELI
                     AENRHLRQLLDMKERVGPASAAAEILYFGRDPFSRKVIIDKGSSNKVEEGAAVVDDTG
                     LIGQVSRAFPWTAEIALITDREQAVPVQVVRNGLRAAVFGTGYDGALDLRFMPVNADI
                     EHGDVLVTSGIDGVYPAGLPVAVVASIERNAAYPFARITCTPAAGVNRHRQVLVLARL
                     APLPEYPPAAQQKKVKPRKSRRDG"
     gene            complement(11833..12876)
                     /locus_tag="VE030_05825"
     CDS             complement(11833..12876)
                     /locus_tag="VE030_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HZF22967.1"
                     /translation="MFGFLNGYFSNDLAIDLGTANTLIYVQGKGIVLNEPSVVAIRQE
                     GGPNGKKVIQEVGLAAKQMLGRTPGNITAIRPMKDGVIADFTVTEQMLKMFIKRVHDS
                     RLFSPSPRIVICVPCGSTQVERRAIRESAYGAGARKVELIEEPMAAAIGAGLPVEEAT
                     GSMVVDIGGGTTEVGVISLGGIVYSGSVRVGGDKFDEAIINYIRRNYGMLIGETTAEE
                     IKKEIGSAFPGSEVREKEVKGRNLAEGIPRSFTISSNEILEALTDPLNSIVSAVKSAL
                     EQTPPELGADIAEKGMVLTGGGALLRDIDRLLMEETGLPVIVADDPLTCVVRGSGRAL
                     EKIDKLGTIFTND"
     gene            13056..13343
                     /gene="gatC"
                     /locus_tag="VE030_05830"
     CDS             13056..13343
                     /gene="gatC"
                     /locus_tag="VE030_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011353557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HZF22968.1"
                     /translation="MALTLSDVKRIAHLARIEVTDAEAGQALGQLNDIFRLIEQLQSV
                     DTSGIEPMSHPLGGSQRLREDVVSSTENREANMRNAPSQLDGLFLVPRVIE"
     gene            13354..14811
                     /gene="gatA"
                     /locus_tag="VE030_05835"
     CDS             13354..14811
                     /gene="gatA"
                     /locus_tag="VE030_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HZF22969.1"
                     /translation="MFKKSVAELGRALIAGEVSAVELARFFLERIESHKDLNAFLDVR
                     PEVTLAQARAADARIARGEATPLTGVPIAHKDIFVTRDFHSTASSRMLEGYLSPFDAT
                     VVEKLGSAGMVALGKLNCDEFAMGSSNENSHYGNVLNPWDKTAVPGGSSGGSAAAVAA
                     RITPAATATDTGGSIREPAAFSGVTGIKPTYGRASRWGMIAFASSLDQAGIMTRSAED
                     AALIFNAMLGFDPKDSTSVERAHEDYTRSLDVDLKGLRIGVPKEFFGDGLQPDVEKAV
                     REALAEYVKLGVKLVDISLPNAALGIPVYYVIAPAECSSNLSRFDGVRYGHRAARYSD
                     LIDMMKKSRSEGFGPEPKRRILIGTYVLSHGYYDAYYIQAQKVRRIIADEFQRAFREC
                     DLIAGPVTTSVAFGFGEKAADPVAMYLSDLYTIPGSLAGIPGMSIPCGFGAKNRPVGL
                     QLMANYFDEARLLGAAHRYQQATDWHLRVPPAAKE"
     gene            14896..16428
                     /gene="gatB"
                     /locus_tag="VE030_05840"
     CDS             14896..16428
                     /gene="gatB"
                     /locus_tag="VE030_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HZF22970.1"
                     /translation="MDWEIVIGIETHAQLSTRSKIFSRASTAFGAAPNTQACAIDMAL
                     PGTLPVLNKGAVERAIRFGLAVGGTINPRSIFARKNYFYPDLPKGYQISQYEIPIVQG
                     GSLAIETKGARKVIRLTRAHLEEDAGKSIHGEALSGVIGKGVVGRMVVGDSIDLSNVS
                     AIDLNRAGTPLLEIVSEPDMRSAEEAVAYAKTLHVLVRWIDICDGNMEEGSFRCDANV
                     SVRRPGAPLGTRREIKNLNSFRFLQQAIEYEVKWQIETLEDGGSIQQATVLFDPESGE
                     TRMMRTKEDAHDYRYFPDPDLLPLEVSETWIEDCRTKLPELPEAKRKRYEDGYHLFSQ
                     DAVALTASIDTANYFEAVLAELRKIPGIAYGTQSPDAANAKHVANWVINDVGALLNRN
                     DLSILQSPVSASQLASLLARVFDNTLSSKLAKTVFEAMWAGEGSADEIIEKRGLKQIS
                     DSGAIGKIVDEVLGANPQQVADYKGGKEKAFNSLVGQVMKASKGKANPALVNEMLKNK
                     LA"
     gene            complement(16436..17050)
                     /locus_tag="VE030_05845"
     CDS             complement(16436..17050)
                     /locus_tag="VE030_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22971.1"
                     /translation="MKVIRSLVSLTIATALAVPALAQQKPTPGGSGPVTYKCVDGRGK
                     IYYSDQFTPECGQTQEMNRQGRILKKHETAKPAADGTGGAKDSAERQRRDRALMSTYT
                     SEEEIDLARDRSLMIPLQAVKTVENRLDKANSQLFDLKSQADRLAGKQKAIPPNLLEE
                     IDGKQKEVSALEAELQQKKSYADSVRARYEGDKLRYRELNTASK"
     gene            complement(17047..17691)
                     /gene="pyrE"
                     /locus_tag="VE030_05850"
     CDS             complement(17047..17691)
                     /gene="pyrE"
                     /locus_tag="VE030_05850"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HZF22972.1"
                     /translation="MKNFRQEFIEFALARGVLCFGEFKTKAGRLSPYFFNAGLFNDGE
                     ALRRLGQFYAKAIVAADATFDMLFGPAYKGIPLVSATAVALAEIGRNLPFCFNRKEAK
                     DHGEGGMTVGAPLAGQVLVVDDVISAGTSVRESVQIIRAAGAAPCGVIIALDRMERGS
                     GTQSAAQEVRSQFGIPVISIATLADLTVFLRARADMARQLQAVERYRKEYGVSA"
     gene            17691..18476
                     /locus_tag="VE030_05855"
     CDS             17691..18476
                     /locus_tag="VE030_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008647203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HZF22973.1"
                     /translation="MPDVRIITLNLNGIRSAVNKGFLEWMSAQDADAICVQEIKAQDR
                     DLVEAIRNPDGYRGYFHCADRPGYSGVGIYCRKKPDRVIEGLGIPDIDAEGRYLEAQF
                     GNLSIVSVYVPSGSSSAERLAIKFSFMERFIAQLHRLAQSGREVILCGDWNVAHKEID
                     LKNWRSNQKNSGFLPEEREWFSGVLDKLGYVDVFRRLNQEPEQYTWWSNRGQAWAKNV
                     GWRIDYQLATPGIAAKARRAAIYKAQRFSDHAPLIIDYDYPLA"
     gene            18606..19964
                     /locus_tag="VE030_05860"
     CDS             18606..19964
                     /locus_tag="VE030_05860"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZF22974.1"
                     /translation="MDQNTTTALRDWRSRLPEGVRPYFESAPLAALFLGISSGFGFAM
                     IGATLTTRLAQHGITKSAVTAFALTVLAYNFKFLWAPIVDNVRLPVIGRFGQRRSWLW
                     LVGVLVMAAVAFLGIADPDEALYIVAIAAITVGVAGATFDIIIDAYRIELLEPRQLGV
                     GSGMSQYGWRIGAAAAGALALVIAARLGWSAAYFACAVFALPGMLVGLVMGEPERHRE
                     PAKPRGVVEAIVAYFSPLLEFLKRQGALVVLLFVLIHKIGDTLANLTFRLLFKDLGFS
                     NDEVAYYDVGVGFIALLIGIFAGGILYARLGMKRAVLVSLILMAVSNFSFAALAAVGH
                     TNTGMAAAIGFENFASGMGGVTVVAYLSALCNLRFTATQYALLSALASIAGRFLTGTS
                     AGALIEAIGYVNFYLLTTVIAFPGVILFWFMIRSGLADLSIGSAGKEGKVDARVDKAA
                     DA"
     gene            complement(20099..20719)
                     /gene="metW"
                     /locus_tag="VE030_05865"
     CDS             complement(20099..20719)
                     /gene="metW"
                     /locus_tag="VE030_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HZF22975.1"
                     /translation="MSASAAPGTRSGRSDFAAIAKWIKPNAQVLDLGCGDGSLLRHLK
                     QARGVTGYGIEIDDARVLACVRNGVNVLQNDLESGLSGFESNSFDYVILSQTLQAVRH
                     TERILKDMLRVGREGIVTFPNFGYWRHRLQILAGRMPVSKELPYEWYDTPNIHLFTIR
                     DFQVYCRTHGVRILERLVMAGAKPVSVLPNLLGALAVFRFDAGSRA"
     gene            complement(20716..21858)
                     /locus_tag="VE030_05870"
     CDS             complement(20716..21858)
                     /locus_tag="VE030_05870"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HZF22976.1"
                     /translation="MPPEGSVGVVSPKLAHFDEPIELKSGARLDSYDLAYETYGALNA
                     ARSNAVLVCHALNASHHVAGVYADDPENVGWWDNLIGPGKPVDTNRFFVIGVNNLGGC
                     HGSTGPSSINPATGRPWGADFPVVTVDDWVQAQARLATRLGIERFAAVMGGSLGAMQA
                     LQWAISQPERVRHSIVIAAAPKLSAQNIAFNEVARQAITTDPDFHGGNYYSYGVVPRR
                     GLRIARMIGHITYLSDDTMAEKFGRELREDTLKFNFDVEFQIESYLRHQADKFAERFD
                     ANTYLRITKALDYFDPAAACGGDLSATLKPAKAGFLLVSFTSDWRFSPARSREIIKAL
                     VDNRLGVSYAEIDAPHGHDAFLMDDPRYHNLIQTYFGNIVLQGPGT"
     gene            complement(21904..22485)
                     /gene="lptE"
                     /locus_tag="VE030_05875"
     CDS             complement(21904..22485)
                     /gene="lptE"
                     /locus_tag="VE030_05875"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HZF22977.1"
                     /translation="MKTDVCQRSWSAMKAMNRFLRDFPSPLVPRPSSLVIATFLVAMT
                     LSACGFQLRGQAAIPFQTVRVEAPGFSAFANDLERAIRAGSKTRIVESRDQAEAVVQI
                     VGESQEKHILSLSSGGKVREFELLYRIAYRLTNRAGVDLALPGEIVLRRDMTYDDTVV
                     LAKESEELLLFRDMKTDAVQQMLRRLSLVKPAT"
     gene            complement(22527..25157)
                     /gene="leuS"
                     /locus_tag="VE030_05880"
     CDS             complement(22527..25157)
                     /gene="leuS"
                     /locus_tag="VE030_05880"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012363082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HZF22978.1"
                     /translation="MQPQYQPAAIEQQVQQEWADKKSFRAVDASHKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRYYRMRGYNVLQPMGWDAFGLPAENAAMANNVPPAKWTY
                     DNIAYMKKQLKSLGFAIDWERELATCRPDYYRWNQWLFLRMLEKRLAYKKTGTVNWDP
                     VDQTVLANEQVIDGRGWRTGALVEKREIPMYYLAITRYAEELLSSLDRLPGWPERVKT
                     MQANWIGKSFGVRLAFPYVLGGKAGKLWVFTTRADTIMGVTFVAVAAEHALAAEAARR
                     NPKLAEFVEECKRGGVQEAEIATQEKKGMPTGIFVEHPLTGENIEVWVGNYVLMSYGE
                     GAVMGVPGHDERDFAFAKKYGLRIKQVIDVEGRPYSLDAWQPWYADYGVCVNSARYDG
                     MPYQQSVDMIAADLKAKGLGEKQVTWRLRDWGISRQRYWGCPIPIVHCDSCGDVPVPD
                     DQLPVVLPEDCVPDGSGNPLNKRPDFLNTACPKCGKPAKRETDTMDTFVDSSWYFLRF
                     ACADQHERMVDERVNYWLPVDQYIGGIEHAILHLLYSRFWTKVMRDFGLVKLDEPFTR
                     LLTQGMVLNEIFFRKTDGGRAQYFNPADVEVQFDDQGKRKAAVLKEDGKPLESGGIGT
                     MSKSKNNGVDPQALIDQYGADTARLFVMFASPPEQTLEWSDDGVEGASRFLRRLWLFA
                     AGRGKDNTKFNLSSAFISITDWSSQPSAIRDFRRGIHLSLKQASYDYERMQYNTVVSA
                     CMKMLNLLEGFSGGQGLGSVNAVSQASSEALDILLRVLYPVTPHITCVLWKELGYGAD
                     IIDAPWPAVDEAALVQDEIDLVLQVNGKLRGNIRVARTADRAAIERLAVENPNAQKHI
                     AGQTVKKVVVVPGRLVNIVV"
     gene            25272..26150
                     /locus_tag="VE030_05885"
     CDS             25272..26150
                     /locus_tag="VE030_05885"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HZF22979.1"
                     /translation="MLIRAGIAALLIAAAMPLQAAPDAMVEGVQLPAWLIRDGQREPL
                     TAGTELKARDEIATGSNSRLLLRLGDGSLVKLGENGWLQIAELAQRQAKNFLSATLKV
                     FAGAFRYTTAPEPAADSEVTVQFTTLIARFNAADIWGKKLSDHQVLVLIDGKVSVARV
                     AESSASPPLDMKDALTFLQAPRSGAGKIEPVQIEQLKIWAAETEISEGQGAIRRGGLW
                     KLNLAKYANQIQALSLYDSLRREGYPALIQQEPREEGPAYLVRIAGFPSELEANLLGA
                     RLKQINPRLEPIASLQ"
     gene            complement(26139..26261)
                     /locus_tag="VE030_05890"
     CDS             complement(26139..26261)
                     /locus_tag="VE030_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22980.1"
                     /translation="MKAVFFDPRRIDALALEDAFEGLPLGRRFGGGRGYKKLLQ"
     gene            26456..28642
                     /locus_tag="VE030_05895"
     CDS             26456..28642
                     /locus_tag="VE030_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HZF22981.1"
                     /translation="MSILEGLNPQQLEAVTLPHQSALILAGAGSGKTRVLTTRIAWLI
                     QTGQIGPLSVMAVTFTNKAAREMLTRISAMLPINTRGMWVGTFHGLCNRILRAHYRDA
                     GLPQTFQILDSADQLAVIKRVMKAMNVDDEKYPPRQAQNFINSAKEQGLRAAQVDAPD
                     DFSRRLLTVYEAFEAQCNREGVVDFAELLLRCYELLGRNELLRDHYRGRFRHILVDEF
                     QDTNRLQYHWLKLLAGPNNTIFAVGDDDQSIYAFRGAHAGNMLDLERDFGIEKVIKLE
                     QNYRSHGNILDAANALIRHNRTRLGKNLWTSEGGGEPLRVYQAMTDIEEAAYIVDEVR
                     QLHNEGVALSDMTLLYRSNAQSRVLEHALFSAGISYRVYGGLRFFERQEIKHALAYLR
                     LTANPDDDGAFLRVVNFPTRGIGNRSLEQLQEAAAGRGISLWQAACANTPSGKAGASI
                     AAFIQLIEAMRKACENLPLPEIVDHVVATSGLIAQYQTEKEGADRIENLNELTNAAAA
                     FVGQDGPITLISAEGEIRSEGLDPLNAFLAHAALEAGEHQAGEGADALQLMTVHSAKG
                     LEFHTVFISGLEEGLFPHENSVSEQDGLEEERRLMYVALTRARRRLYLTHAQTRMLHG
                     QTRYNVPSRFFDEIPDELLKRINTTKFSGFVAAKGYEAGSKARAPMVETGASGWRIGQ
                     SVVHPKFGAGVIVNAEGRGSDARVQVNFRDAGLKWLALDYAKLVAA"
     gene            complement(28654..29379)
                     /locus_tag="VE030_05900"
     CDS             complement(28654..29379)
                     /locus_tag="VE030_05900"
                     /inference="COORDINATES: protein motif:HMM:NF041043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HZF22982.1"
                     /translation="MNIRSVAPGRGWQWITAAFQLFRKNPVIWVVLNMALMLIGVGLA
                     MLPVIGTYVLYLLTPVFLGGIMAAAKDLEAGQDIEIAHLFRGFRHNAAHLVTVGGVYL
                     VGQVVISGVMLTVGGPEFQESVKAGIAALDASALTPEGATRIFQAMLVGTVLFMPLAM
                     AVWFAPALVILDDQPGFQALWTSLLACFRNALPLLLYSIVSSVLLLFAVIPFGLGLIL
                     WIPVMLLTIYTSYRDVFVSGQAN"
     gene            complement(29396..30361)
                     /locus_tag="VE030_05905"
     CDS             complement(29396..30361)
                     /locus_tag="VE030_05905"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF003558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HZF22983.1"
                     /translation="MSVFTTVTPEQLSAWLRNYSIGVLTDLQGILSGIENTNYFVTTT
                     HGKFVLTLFEKLTRQELPFYLNLMAHLSSHGLPCPKPIANLQNELLGTLNGKPAAIVT
                     CLEGQQVTEPTPEHCAQVGGVLADMHLAGRTFKSHLDNLRGEKWWTACAPEIYPFLTA
                     EDAELLESEIRFQAQQVREGLPRGVVHADLFRDNVLIQHDQNHGPHVGGFIDFYFACV
                     DCFIYDVGITVNDWCTNPDATLDPARTQALLKAYTAVRPFTDQERDAWPWMLRAGALR
                     FWVSRLYDFHLPRPGELTHAHDPERFRRILLNHRKAADRPQRQTV"
     gene            complement(30369..31097)
                     /locus_tag="VE030_05910"
     CDS             complement(30369..31097)
                     /locus_tag="VE030_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004530319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HZF22984.1"
                     /translation="MSDKQKVPVPLAPELVEKAWSARESWRVFGIMAEFVEATERLAN
                     VRPAVSIFGSARIPAGHPYYRLTEEIARQLSDAGFSVISGGGPGVMEAANKGAFFGRS
                     LSVGLNIQLPHEQISNQYQDVSQTFRHFFARKVMFVKFASAYVVVPGGFGTMDELMEA
                     LTLVQTQKIRRIPIILVQESYWRGLIDWMRDRMISEGMIDAADMDLIKVIDEPRQVVE
                     AIFSHYEKRGFEPSAAEREAQLNL"
     gene            31123..>32607
                     /locus_tag="VE030_05915"
     CDS             31123..>32607
                     /locus_tag="VE030_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006409467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HZF22985.1"
                     /translation="MKTLLLVDGSSYLYRAFHALPDLRNRNNEPTGAVYGVLNMLRRL
                     RKETAADYIACVFDAKGKTFRDDWYAEYKANRPSMPEELAAQIEPLHRSVRAMGWPLL
                     MVEGVEADDVIGTLAKQAEAKCVQTVISTGDKDITQLVSPKVRLVNTMSNENLDEKGV
                     EEKFGVKPEQMVDYLTLVGDSVDNVPGVEKVGPKTAVKWLKQYGTLDQVVAHANEIAG
                     VVGENLRKALDWLPQAKKLLTIKCDVELPVKLEDLTPQPQDTAKLSELFDRLEFKSWL
                     KELQARGPGHEARGEAESPASSPHALDSGPDAAPLTPHASPARNYETILTDEQLRAWL
                     SRIEAAELVSIDTETTGLDPMTARLVGISASVEPHHAAYIPVGHNYAGAPEQLALDRV
                     LAVLKDWMQSPKHFKVGQNLKYDKHVFANHGVDLAGIVHDTLLQSYVLESHQSHDMDS
                     LAARHLGIKTISYDEVTGKGASRIPFEQVALERATEYSGEDADVT"
BASE COUNT         6349 a        10247 c        10039 g         5972 t
ORIGIN      
        1 ctgaaaaaca acccgctcgg cagaaacgaa tcgctcactg agcgatcgcg gaaccgttgg
       61 ctggagtcgg ccgatcgaaa gccacggatg cggccggtag caccttcagc cgccactctc
      121 gcaagttctt cagaaccacg ctccctgtca tgctcacgct ctcctccgga tccagactga
      181 tattgaacgc gtattccttg cctgttctgg agttaatcca gtacttccat aagttccggg
      241 gatcgcggtg atcaaacagc cccacttccg attgctcctg gaaatagctg aattccccgt
      301 cggaggcgtt ttgcatgggc agacccaccc acgtcgcggg gcggggcatg ccaagctccg
      361 cgaagatggt gggagcaata tccacctggg acgccgttcg cctgttgatt ggccggccat
      421 gctggcggcc atatgaaatc aacaagaagg ggatgcgcag aacctgctca tacacgctat
      481 ttgtgtgttg aaaaacccca tgttccccaa gcgattcgcc atgatccgcc gtaacaacga
      541 ccagggcatc attgagatag cctttacccc gaagaatctc caggattgtg tgaatttcaa
      601 gatccgcctg cagtactgca ttgtcgtaat aattggcaat gcgttcgctg acgcgccctc
      661 ccgttgcgga atcctgattt tcaggaagtg catagttggc agccggcaga tatttgttcg
      721 ttatcccatg ccgcttgccc aacaagtgcg tagccatcaa gtgaaaatga atcatgaccg
      781 gttctttgtc ccacaccggg aaagtaccga gccggtccag cacaagctga tcatcgttca
      841 tatatctgaa gcgccgggcg gacatgccat catagtaaga gtcgacatca ccatagatgc
      901 gcttcaaggc ataaaaattc gtatggtcgc cgctcaagac catatggacc cgatagcctt
      961 gtctcttcag ggcctcatgc aaaatgaagg gatgattgga aaactgatgc acaaattttg
     1021 aacttgcaag gctcagcagc ccgcatgcgg aactggagca cgatgcatgc acggtgagcg
     1081 ccttgcgcac cattccggca ttcatgagcc ggctcaggtt gggcgttgtg tcgcgttcat
     1141 atccatagac tcccatgtgg tcgggccgca gtgcatcgac aacgatcaat atcagatttc
     1201 tgcggcctgc cttgagcatt ggtttgtagg aggcccgtgc agattcttcc atgcggtcac
     1261 ggtttcccga gctcagcctg tcgatggcgt gcccttggag gttgcgagcc gcctccattg
     1321 ggaaaaaggt aaggctgatc ggttcaaggt ccagaaccca gggagctgcg aggaagctat
     1381 agcgctcgat cgccagaacc atgcaaccag aaaccagaaa aagtgccaac agccacttcg
     1441 gaccagtcgt tgaaacaaac ggtgcccagt caaagtagta aaaataagtc cacgtaacgc
     1501 ccagcaatcc cagatatgcc agtacgaaac cagctatggc gctggtggcc gaaatttcaa
     1561 gtgcgtcggc aagttccagg acttgcgaac tgtagcttgt aatcaagtcc catgagatca
     1621 cccgccccca cgactgcagg ccgatgagca ccagtgcata gtaggagaag atggtcgcga
     1681 gcaaagcaga cgtaacaata gaaactgcaa agcgcgacaa ggcccggctt ggaatcagga
     1741 aggacaacag caacctgaca aggaccagac ccagcaacac caacgccatc atgcgaacat
     1801 gcggcaatac cgcatcggca gactctgagt agtgattgac gtacacgaac aggaaaaccg
     1861 ccggcatgca gaaccaggcc gctatatcca ttcccacgcg gcaaagcaat tgcactgggc
     1921 gcagatcaga cattccggat ctcgaccagc ctggacagcc cgaagaaatt gacttccttt
     1981 accgaccgga tttcccagtc gtatttgaga atttgatccg caaaactgaa gtcaaaacga
     2041 aaacctaccc tgctcgctat tgactgcagg gcttgttcga gcaaccacca gaagcggctg
     2101 ccgctgaagt gattgaggat caggattgtc ccgtttttcc ggcacaccct tctggcttct
     2161 gcgatgagcc gctccgggtc aggcgtcacc gacaaggcat agggaatcgt gacgcaatca
     2221 aagaatccat ccggaaaatc cgtttcctct gcatccattg cctccaggcg gatatcacgc
     2281 ccggtcagcc gcctcacacg ctgccacgca atctccagca tttcggccga tatatcgatt
     2341 ccgacgactt tcgagtaggc agggtatcgg gccagcatca gcccggtacc taccccgatt
     2401 tccaatagcg aggccggacg cagcaaatca accgcctccg acatcgcgcg cctcccgggc
     2461 tcaagcacag atccataaag atagtcgtaa aaaggagcgt agaaccggta ggcacgaatg
     2521 acgttgtctt gcgatatctt ttgcatcgaa gtgtttaatg aaagagatcg ggggctggca
     2581 tcgagcctcc tgcaaagatt cgcattggtt tttttccgcc cacacttgtc cccgatggca
     2641 gcgggattag tggctgcggc cttaggaagt caaatcggga acaagggcgt gacagttgaa
     2701 gaatctggcg cgcccggcgg gagtcgaacc cacgaccttc ggcttcggag accgacactc
     2761 tatccagctg agctacgggc gcaatgaggc gctagtttac gggcaaactt ggttttgcgt
     2821 ccatcggggt cctgtttcgg acagcgcgga tgacctataa tccgcccctt ccaacgcgtc
     2881 aatccaaggg tttttcatgg ccgaggtcca catggaggag cgctcgtcgc caatcaagac
     2941 gccgaagcaa ctcgtcgtcg ccatcgtgct cgccttcctg gtaccggtac tcgggatcgt
     3001 catgatcgca gagctcgcca ccagcgggct gaaggttgat ccaaagagtt ccgcgatgag
     3061 cgacgaagca gttgcacggc ggctcaagcc ggtcggcgaa gtcgtcatcg gggaagccgg
     3121 cgcccccgtg cccgccacca caacaaaatc gacggccggc ggcccgacgc agatcgcggc
     3181 tgcggcgaag tcggcgggcg gcgcggcaga cggcaagaaa acctacgatg cggtctgcgc
     3241 catgtgccac gctgccggag tcgctggcgc tcccaagact ggcgacaagg cggcgtggaa
     3301 gccacgcatc gcgcaaggca aggatgcgct gtacaccagc gcactcaagg gcaagaatct
     3361 gatgccgccg aaaggcggcg cagcctcgct tccagacgcc gacatcaagg ccgcggtgga
     3421 ttacctggtc agccaggcga agtgattcgg taaatgggaa atagggaata gtgaactgtc
     3481 ctggagggcg cagctttttc ctgcgccttt tttgttttca tgcgtcttgt gcgacattac
     3541 ccatttacca ttcaccgttc accattcacg gcttccaaat gtcgacatac gcgataggcg
     3601 atctgcaggg ctgttacgat ccgctgcggg aactgctcga agagatcggt ttcagcgcga
     3661 cacgcgaccg cctgtggttc gtcggcgatc tggtcaaccg cggcccgcag tcgcaggagg
     3721 tgctgcgttt cgtcaaaggc ctcggcagcc gcgcggtcac ggtgctcggc aatcacgatc
     3781 tgcatctgct gatggtcgcc gagggccgcg caaagttgca caagggcgac actcttgccg
     3841 ccatcctcga ggcacccgat cgtgaagaac tgcttgcatg gatgcggggg cttcagttga
     3901 tgcacgtgga aggcgagtac gcgatggtcc acgccggatt gctgccttcg tggagcgtgc
     3961 ccaaggcgct cgacctcggg cacgaagccg aggaagttct gcgcggtccc gggtaccgga
     4021 gcctgatggc gcacatgtac ggcaaccagc cggatcgctg ggatgatgcg ttgaccgggt
     4081 atgaccgcct gcgcgtgatc atcaacgtga tgacccggct gagaatatgc acccccgacg
     4141 gccgcatgga gttcagccac aaagggcggc tcgaggacat cccggaaggt tacgttccgt
     4201 ggtttgcggc gccgaaccgc aagagtgcca acgcgaccgt tatctgcggt cactggtcgg
     4261 cgctcggctt gctggccgag aaaaaccttc tggccctgga ttcgggttgc ctgtggggcc
     4321 ggcgcctgtc ggcggtccgc ctggaggatc ggcggacatt ccaggtcacg tgcccgaagt
     4381 tcgccgacta aacgcggcag tgattgccgc gtgcgcctcg cgcgcaaggt cccgccgcga
     4441 cttgccttcg gcatcgattg ccggcaggaa aatcatctcc gccacgatct tgcgttcgcg
     4501 caggatcatc gccatggatt cgatcaggct ggtgtctccg acatagctgg cgttgacgtt
     4561 caccctgcgt ttttcgtcca ggtatcgcag cgccaccggg aacaccaatg cgcgctcgtc
     4621 caccgcaggc tgcagcaggg aagcgtggaa gggttgcagg ccggtgccgt cgctggtcgt
     4681 gccttcggga aacacgccga tggcgtcccc ggcgaggaac gccgcgtgga tcgcctcatt
     4741 gatgcgcgcg gcgtggcggc ttttgccgcg ccggatgaac agcgtcccgg tcctggccga
     4801 aagccagccg atcagcggcc accggcgcac ttccgatttg gcgatgaagc gcacctgcca
     4861 caccgaatgc accagcggga tgtcgagcca ggaaacgtga ttcgcgacca gcaccgctgc
     4921 acgatccctg acctcgggag gaacgccgcg caagcgcggc tcgacgttga cggcggcgag
     4981 gatgcgcgcc gaccagcgcg ccacggccag ccggcgccgc gcgtttccat agaaaggaaa
     5041 cagcagggcc acggtggcaa tgccggcgaa gaggtggaac agaagcagcg tcgcgcgata
     5101 gaaacggacc ggaatcgagg tctgttctct atcgtggagg gccatgcttc aggaaatgcc
     5161 ggcagcgggc aggacgccgc tatttgcgct caccacggcg atctgccgaa cgcgtcgcga
     5221 aaacgcgcgc cgatttcgtc gcgcgaaaac tggtggttct gcgcgccgcg ttctgcgacc
     5281 ttgaccgatc ccagcagcga ggccagcctg ccggtggtcg gccagtccag atcgttggcg
     5341 atgccgtaca acaagccggc gcggtaagca tcgccgcagc cggtcggatc caccactcgc
     5401 gccggcttga cgctgggtat cctttcgatc ccgccgccgg aatacaccag cgacccctgc
     5461 gccccctgcg tgaccacgaa ggccgtcacc agttttgcca gctcttcaac ggtcctgccg
     5521 gtgcgttcct gcagcagctt cgcctcgtaa tcgttggtag ccacgtactc ggcctgcctg
     5581 atgaaattca acagctcctc gctgttgaac atcggcaggc cctggccggg atcgaatatg
     5641 aacgggacgc cggcctggtg gaattcgcgg gcatgctgca gcatgccctc gcgcccgtcc
     5701 ggcgccacga tgcccaaacc cacgtcgcgt gcatcggcga cacggttcag atgcgaggaa
     5761 ttcatcgccc ccggatggaa cgcggtgatc tggttgtcgt ccaggtcggt ggtgatgaaa
     5821 gcctgcgcgg tgaacgtgcc cgctacctgg cgcacgtgcg cctgcgacag ccgcagcttt
     5881 tccaggcggt aagcgtaagg ctgatagtcc tcgccgacgg tcgccatgat cagcgggtcg
     5941 ccgcccagca tcttcagcgt gtaggcgatg ttgccggcgc agccgccgaa ctcgcggcgc
     6001 aagtccggca ccaggaaggc gacgttgagg atgtggatct ggtccggaag gatgtggttc
     6061 ttgaagtagt cgccgaacac catgatggtg tcgtaggcaa tcgatccgca gatcagtgtg
     6121 cgcatgctcc gccaggcccg ctgatttcag cgtttcagca caaaaggcgt gaatccgagt
     6181 tcctgccgga tgcgttcggc ggcgcgctcg gcctctccgc gattggggta aggccccgca
     6241 tgcactttgt aaagtccttc gccacggtac agttgcatcg aaccggcaag ccagcccagg
     6301 tccatgcgca tcttgcgcag gaatgcgtcg gcgttttgct ccaccgagaa ggcgccgagt
     6361 tgcacgaaga tgccgccggc ctcgttgctc accggcaccg caaccggcgc gggtgccgcc
     6421 ggcacgctcg caaccaccgg ggcttcgccg ctgccgggaa tgatggcttc cacttcaacc
     6481 acggcgctgc cctggccgat gatgccgatg cggtgcgcgg cggcgtagga aagatcgatc
     6541 aaccggtcct cgtggaacgg gccgcggtcg ttcactcgca cgaccaccga tttgccgttg
     6601 gcgacattgg tgacccgggc gtaactcggg agcggcagca gcgtgtgcgc cgcactgagt
     6661 gcgtacatgt cgtaaacctc gcccgaggag gtcttctggc cgtggtagcg cttgccatac
     6721 caggtggcca cgccgcgctg cttgtacgca gtcaactgcg tgtaaggcgt gtaggtccgg
     6781 ccgagcaccg tgtacgggcg gctggtgtac ttgttgatcg gctctatttt cggagccggc
     6841 tctggcatcg aatccagatc cgccggcggg ttggcgtccg gcccgtcgtc gagataataa
     6901 ccgccgggct tcgacccggg acgggaggga acgctgccgc agcccgcgat catcatcgca
     6961 acgaagatcg cgatgacgat cgcggaggga cgaggggcga ggaatgaggg gcgagtgaag
     7021 gattcaggca ccgaccgcaa tgcgttcata ggcgttataa attcaagtgc cgagctgtgt
     7081 atgtatgcat ttcccttaat ccctcgcccc tcgtccctcg cccctacgtc tgcaccagac
     7141 gtcgatgcgt atggatgctc atgagcaatc cgacgctgaa tagtatcgtc accagcgagg
     7201 tgccgccata gcttatcagc ggcagcggca cgcccaccac cggcagcatt ccgctcacca
     7261 tccccatgtt gacgacggcg taagtgaaga aggtcagcgt gatcccaccg gcgagcagcc
     7321 gggtaaacag cgtcggggcg ttggctgcaa tcaccagccc gcggccgacc accagcagga
     7381 acaacaccat cagcaccagg ttgccgaaca ggccgaattc ttccgcgtac accgcgaaga
     7441 tgaagtcggt ggttcgctcc ggcaggaagt cgagatgcga ctgcgtgccg ttgagccagc
     7501 ctttgccgaa aaatccgccg gatccgagcg cgatggtgga ctggatggtg tgatagcccg
     7561 cacctagcgg gtcctgcgag ggatcgagca gcgtcaggat ccgctggcgc tgataatcgt
     7621 gcagcatgga ccacaggaac ggaaggctcg ccccgcccgc caacgcaagg cccaccagga
     7681 ttttccacga cagcccggcc agaaacagta cgaagaatcc ggccgcggtt accagcagcg
     7741 cggtgccgag gtcgggctgc ttcgcgatca gcagcaccgg caccagtacc agcagtgctc
     7801 ccacggcgaa gtcgcgcatt ttcagcaccg cctcatagcg gtcgaagtac caggccagca
     7861 tcaagggcac cgcaattttc atcagttccg agggctggat gcgggtgaat ccgagatcga
     7921 gccagcggcg cgcgccgttc acgatctcgc cgaacgccgc caccgctatc aggagcgcca
     7981 ggccgagcac gtacaacggc agggcgatgc gggagaggta atgcaagggc acgttggcgg
     8041 cgatccacat caccaccagc gcagcgcaga tgttgcccat ctgcgccgct acgcgctcga
     8101 ggctcgtccc cgaggcgctg tacaggatca gcaaacccat caccagcagg atggccacca
     8161 aggtcagcag ataacggtcg aggtgcttga ccagataggc gtacagacgg ttaatcacct
     8221 tcgcgctccg ggtccggttc cagcacgtgc tccagcgccc gctcctgctc cggtgccagc
     8281 tccgggaccg tttcctgggc cggttctcgc accggtttcc tggtcgccgg cttcaccggt
     8341 tgcggctcct tgcccagcag atagaaatcg atgacctggc gcgcgatcgg cgcggcggtg
     8401 ctgctgccgt gtccgccgtt ttccaccagc acggccagag cgatcttggg atcgtctgcc
     8461 ggagcgtaac cgatgaacag cgcgtggtcg cgatggcgcg catggacgcg atcctcgtcg
     8521 tatttctgcc ctttcaatcc gatcacctgc gcggtgccgg tcttgccggc aaacaggtac
     8581 tgcgctccca tgcccgccca ggccgcggtt ccgcccggtt tcatcacgtc caccatcgcg
     8641 tcgcgcacgc gcttgacgtt ctcgggcttc aacgccactt cgccgatcgg ctccggctcg
     8701 atgctggtca actcgccggt ctggccgtcc tgtatgtgct ggaccacgtg cgggcggaag
     8761 atacggccgt cgttggccag aatcgccgtg gcgaacgcaa gctgcagcgg cgtggtcagg
     8821 ttatacccct ggccgatgcc caccgagatg gtgtcgccta cgaaccactt ttgctggaag
     8881 cgccgctgtt tccattcttg agagggcaac agcccgccga cctcgccctc gatgtcgatg
     8941 ccggtcgtac tgccgaggcc gaactggccg atgaattcgt ggatgaaatc gatgccgagt
     9001 tcgtgcgcca ggccgtagaa ataggtatcg caggactcga cgacggcctt gtgcaagttc
     9061 acgatgccgt gaccgcccgc cttccagtcg cggtagacgc gcccgctgcc gccgccgccg
     9121 agcacgaaat aacccggatc gctgatggta aacccgggtt tgcgctttcc cgactcgagc
     9181 ccggccagcg ccatgaaggg cttgaaggtc gagcccggcg gatacacacc ctgcaaagcg
     9241 cggttgttga gcggatggtc gggtgaattg ttcagcgcct cccagttctg cggatcgatg
     9301 ccctcgacga acagattggg gtcgaagccc ggtctggata ccagcgccag aacgccgccg
     9361 gtggcaggct cgatcgcaac cagcgaaccg cggtgagtgc cgaacacctg ctccgccacc
     9421 tgctgcagcc gcgcatccag tgccagccgc aggtcgttgc cggacaccgg caggatgctg
     9481 cgcagtgtgc gcaccgagcg cccgttggcg tcggtctcca cgtgttcgta gccggttacc
     9541 ccgcgcaggt ctttctcgta acgctgctcg agcccgacct tgccgatgta gtccgaggca
     9601 ctgtagttcg ccgccatccc cttgtttttg agatccgcca cgtccggatc gctgatccgg
     9661 ccgatgtaac cgaccacatg cgaggcgatc tcgccattgg ggtagtggcg gaacagccgc
     9721 gcgttcaact ccacgccggg gaaacggtat gcgttgaccg cgaagcgcgc cacctcctcg
     9781 tcgtttagcc gcgtgcggat cggcacgctg gcaaagtcgt ggccttcctc gagcagtttc
     9841 tggaaacgct tgcggtcctt gggggtgatg tcgaccagta tcgacagcct gttgatcatg
     9901 tcatcgaagt cagccacctg cgtgggcatg atctccaggg tataagcggc aaagttgtgt
     9961 gccagcacca cgccgttgcg gtccacgatc aacccgcggt tgggcaccac cgggacgatg
    10021 aaaatgcggt tctgctcggc cagcgtatgg taatagtcgt gctggatcac ctgcacgtag
    10081 aaaaaacgca cgaacaggac aaagaaggcg aacagcacgg cgccgccggc gatcgccagt
    10141 cgcagttgga agttgtagag ctcgcgttga tggtcgcgaa gctcgactgc acggttcacc
    10201 gcggctcggg ggaatgtcgc caggcgcatg cgcggcggac gccttagacc tcgtcaggat
    10261 cccgccgcga gcgcagcgga atcgtgaaca ggaaccgggt taccggccac agcagcgcac
    10321 cgacaacgct gggcacgaaa taccaccagc ccgggtactc gccgcccgcc gccgcccgca
    10381 ccgccagcac gatcacttgc atcagcagca ggatcaccag cacgtgcgga atctggtggc
    10441 gcacgccgaa catcgccacg cgccggtgca aggcgaaggc gctgaacatc atcaccgcgt
    10501 aggccagcgc gtgctggccg aacaggctgc cctcggccac gtccaccgcc aggccgagca
    10561 gccaggcggg aaggaacccg atcttgcgcg gctggttgat cccccagtac agcaacgtga
    10621 gggcgacgaa atcggggcgc agcgccagca cccagccggc aagcggcagc aggttcagca
    10681 gcagcgcggc agccagggtc acaacgacgt acagcggtct gaccggaagc aggatctcct
    10741 gggtcgaaag gttgctgaat ccggagccgc gcatcagccg tccctgcgcg atttacgcgg
    10801 cttgaccttc ttttgctggg ccgccggggg gtactcgggc agcggcgcca gcctggcaag
    10861 aaccaggacc tggcggtggc ggttgacgcc ggcggcggga gtgcaggtga tgcgcgcaaa
    10921 agggtaggcg gcattgcgtt ctatgctggc aaccaccgcc accggcaatc cggccgggta
    10981 aacgccgtcg atgccggagg ttaccagtac gtcgccgtgc tcgatgtcgg cgttgaccgg
    11041 catgaagcgc aggtccagcg cgccgtcgta gccggtgccg aaaaccgcag cacgcaggcc
    11101 gtttctcacc acctggaccg gcacggcctg ttcgcggtcg gtgatgagtg cgatctcggc
    11161 agtccaggga aatgcgcggc tcacctggcc gatcaatccg gtgtcgtcaa cgaccgcggc
    11221 accttcctcg accttgttcg acgaaccctt gtcgatgatc accttgcggc tgaagggatc
    11281 gcgcccgaag tacagtattt ccgccgccgc cgaggccggt ccgacgcgct ccttcatatc
    11341 caggagctgg cgcaggtgac ggttctcggc gatcagttcc tgttgtgtct gcaacagccc
    11401 ggcgttgagg aaattctcgc gcaccaggcg ctcgttctcg cgctgcaggt aggattggct
    11461 gacaaagaag ctgccgatgc ctgaagccac ttctgccggc acatccacga ttcgctgcaa
    11521 aggataaagc accacgccga tcgcctttcg caacccctcc atgtagtgga agcgcgcatc
    11581 ggcgtacagc aggaccaggg agagcaacac gaagaacgac agtcttgcaa tgaggcttgg
    11641 gccccgcttg aagaacggcg gtggggtgta ctccatccta gggacaaggg gcgagggacg
    11701 agggacgagc ggtgaagcct aatgaagaaa actgatgctt gagaaaagct ctgcgaagtg
    11761 ccgacgcgat tcctgtagta atccgccgtt ccctcacccc tcgcccctca cacctcgccc
    11821 ctcctgaaat cctcagtcgt tcgtgaatat ggtaccgagt ttgtcgatct tttccagggc
    11881 ccggccggag ccgcgcacca cgcaggtgag cggatcatcg gcgacgatca ccggcaaccc
    11941 ggtttcttcc atcagcagac ggtcgatgtc gcgcaacagc gcgccgccgc cggtgagcac
    12001 catgcctttt tcggcgatat cggcgccgag ttccggcgga gtctgctcga gcgccgactt
    12061 gaccgcgctg acgatgctgt tgagagggtc ggtcagtgct tccaggattt cgttgctgga
    12121 aatggtgaag ctgcgcggga tgccttccgc gaggttgcgg cccttgactt ctttttcgcg
    12181 cacttcagag cccgggaatg cggaaccgat ctccttcttg atttcctcgg cggtggtttc
    12241 gccgatcaac atgccgtagt tgcggcggat gtaattgatg atcgcctcgt cgaacttgtc
    12301 gcctccgacc cgcaccgaac cggagtagac gatgccgccc agcgagatca ctccgacttc
    12361 cgtggtgccg ccgccgatgt ccaccaccat cgagccggtg gcttcctcca ccggcaggcc
    12421 ggcgccgatt gctgccgcca tcggctcctc gatcagctcc accttgcgcg cgccggcgcc
    12481 gtaggcggac tcgcggatcg cccgccgctc cacctgggtg gagccgcagg gcacgcagat
    12541 gacgatgcgc ggactggggg agaacaggcg cgaatcatgg accctcttga tgaacatctt
    12601 gagcatctgt tcggtcacgg taaagtcggc gatcacgccg tccttcatcg gccggatggc
    12661 ggtgatgttg ccgggcgtac ggccgagcat ctgcttggcg gcgagcccca cctcctggat
    12721 cactttcttg ccgttcgggc ccccttcctg ccggatcgcc actaccgagg gttcgttgag
    12781 aacgatgccc ttgccctgca cgtagatcag ggtattggcg gtgccgaggt cgatggcaag
    12841 gtcgttggaa aaatagccgt tgagaaatcc aaacataaag taagcggtgg tgccgccgcg
    12901 gtgcgaaaaa tgaggaggtt atgataccct atcgtactgc ggcggttaac aaaaaacggg
    12961 tttcccagga gccacccggg cgcggccacc ggccttgtgt cctccttcct gtcccgttcc
    13021 tgaacccagc cgcgaccacg gaagcctccc acgaaatggc ccttactctc agcgacgtca
    13081 agcgcatcgc tcatctcgcg cgcatcgagg tgaccgacgc ggaagccggg caggcgctcg
    13141 gacagctcaa cgacatcttc aggctcatcg agcagctgca atcggtggac acgagcggaa
    13201 tcgaaccgat gtctcatccg ctcggcggtt cgcagcggct gcgcgaggac gtggtcagtt
    13261 cgacggaaaa ccgcgaggcc aacatgagga acgcgccttc gcagctggac ggcctgttcc
    13321 tcgtgcccag ggtcatcgag taggggtcgc gcgatgttca agaagtcggt ggccgagctg
    13381 ggccgcgcgc tgattgccgg ggaagtctcc gccgtcgagc tcgcccggtt tttcctggaa
    13441 cgcatcgaaa gccacaagga tctcaacgcc tttctggacg tgcgcccgga agtcacgctg
    13501 gcgcaggcgc gcgccgccga cgcccgcatc gccagaggcg aggcgacgcc gctgaccggc
    13561 gtgccgatcg cgcacaagga cattttcgtc acccgcgatt tccattcgac cgcctcgtcg
    13621 cggatgctcg aaggctacct gagcccgttc gacgccacgg tcgtcgagaa actgggaagc
    13681 gccggaatgg tggcgctcgg caagctcaac tgcgacgagt tcgcgatggg ctcgtccaac
    13741 gagaacagcc attacggcaa cgtgctcaat ccctgggata aaaccgcggt accgggtgga
    13801 agctcggggg gttcggcggc ggcggtcgcc gcgagaatca ccccggccgc aaccgccacc
    13861 gacaccggcg ggtccattcg cgagccggcg gcgttttcgg gcgttaccgg catcaagccg
    13921 acctacggcc gtgcgtcgcg ctggggcatg atcgcgttcg cctccagcct ggaccaggcc
    13981 ggcatcatga cccgctcggc agaggatgcc gcgctgatct tcaacgccat gctcggtttc
    14041 gatccgaagg attcgaccag cgtcgagcgt gcccacgagg actacacccg ctcgctcgat
    14101 gtcgatctca agggcttgcg catcggcgtg ccaaaggagt tcttcggcga cggcctgcaa
    14161 cccgacgtcg aaaaggcggt tcgggaggcg ctcgccgagt acgtcaagct gggcgtaaag
    14221 ctggtggaca tctcgcttcc gaatgcagcg cttggcatcc cggtctatta cgtgatcgca
    14281 ccggcggaat gctcctcgaa cctgagccgc ttcgatggcg tgcgctacgg ccaccgcgcc
    14341 gcgcgctaca gcgacctcat cgacatgatg aagaaatcgc gctcggaagg tttcggtccg
    14401 gagcccaaac gccgcatcct gataggcacc tacgtactgt cgcacggcta ctacgatgcc
    14461 tactacatcc aggcgcagaa ggtgcgccgg atcatcgccg acgagttcca gcgcgccttc
    14521 cgcgagtgcg acctgatcgc cggaccggtc accaccagcg tggcattcgg attcggcgag
    14581 aaggccgccg atccggtggc gatgtatctg tcggacctgt acaccattcc cggcagcctg
    14641 gccggcattc cggggatgag cattccctgc ggcttcggcg cgaagaaccg cccggtcgga
    14701 ctgcagctga tggccaatta cttcgatgaa gcccggctgc tcggcgccgc gcaccgttac
    14761 cagcaggcga ccgactggca tttgcgggtt ccacccgcgg cgaaagagtg atgagcgatg
    14821 cgtgatgagt caagaacaag ggcattgaat cgcccctcac ccctcgcccc tcacccctca
    14881 cccgagcgat agcgtatgga ctgggaaatc gtcatcggca tcgaaacgca cgcgcaactg
    14941 tcgacgcgct cgaagatatt ctcgagggcg tcgactgcgt tcggcgcggc acccaatacc
    15001 caggcctgcg cgatcgacat ggcgttgccc ggcacgttgc cggtgctcaa caagggcgca
    15061 gtggagcggg cgatccgctt tggtctggcg gtaggcggaa ccatcaaccc gcgctcgatt
    15121 tttgcacgca agaactactt ctaccccgac ctccccaagg gctaccagat cagccagtac
    15181 gagattccca tcgtgcaggg cggcagcctc gccatagaaa caaaaggtgc acgaaaagtt
    15241 attcgtctca ctcgtgcaca tttggaagaa gacgccggaa agtccatcca cggagaagca
    15301 ttgtctggcg ttatcggaaa aggagtggtt ggcaggatgg tcgtgggcga ctcaatcgat
    15361 ttgtctaatg taagcgccat tgatttgaac cgcgccggca cgccgttgct ggaaatcgtg
    15421 tccgagccgg acatgcgttc cgcggaagag gcggtcgctt acgccaagac gctgcatgtg
    15481 ctggtacgct ggatagacat ctgcgacggc aacatggagg aaggatcttt tcgctgcgac
    15541 gccaacgttt cggtgcgccg cccgggcgcg ccgctgggca cgcgccgcga gatcaagaac
    15601 ctgaattcct tccgcttcct gcagcaggcg atcgaatacg aagtgaaatg gcagatcgag
    15661 acgctggaag acggcgggag catccagcag gcaacggtgc tgttcgatcc cgagagcggc
    15721 gagacgcgca tgatgcgcac taaagaagat gcgcacgact accgctattt ccccgatccc
    15781 gatctactgc cgttggaagt gagtgaaacg tggattgaag actgtcgtac taagttaccc
    15841 gaattgcctg aagctaagcg caagcgatat gaagatgggt atcacctttt ctctcaagat
    15901 gccgtggcgt tgaccgcttc gattgatacg gctaattatt ttgaagcggt tttggctgag
    15961 cttagaaaaa ttcctggaat tgcgtacgga actcaatctc cggacgctgc aaatgccaaa
    16021 catgtcgcca actgggtgat taatgacgtg ggggcactcc tgaatagaaa cgacctttct
    16081 atccttcaaa gtcctgtctc agccagtcaa ctcgctagct tgttagcgcg tgttttcgac
    16141 aacacgcttt cctctaagct ggcgaagact gtttttgaag caatgtgggc gggcgagggc
    16201 agcgccgatg agatcatcga gaagaggggc ttgaagcaga tttccgactc gggcgcgatt
    16261 gggaagatcg tggacgaagt gcttggcgcc aatccgcaac aggtggcgga ttacaagggc
    16321 ggcaaagaga aagctttcaa ctcgctggtc ggccaggtaa tgaaggccag caagggcaag
    16381 gccaatcccg ccctggtcaa cgagatgctg aagaacaaac tggcttaaaa accgcctatt
    16441 tgctggccgt attcagttca cggtaacgca gtttgtcgcc ttcgtaccgg gcgcgtaccg
    16501 aatcagcgta gctctttttc tgctgcagct cggcctcgag cgctgaaact tccttctgct
    16561 tgccgtcgat ctcttcaagc agattcggcg ggatggcttt ctgctttccc gccaggcggt
    16621 cagcctgcga cttcagatcg aaaagctggc tgttcgcctt gtccagcctg ttttcgaccg
    16681 tcttgacggc ctgcagcgga atcatcaggc tgcggtcgcg cgccaggtcg atctcttctt
    16741 ccgacgtgta agtcgacatc agagcacgat cgcggcgttg tctttctgcc gagtccttgg
    16801 cgcctccggt cccgtccgcc gcgggcttgg cggtttcgtg ctttttcaaa atcctgccct
    16861 gacgattcat ttcctgggtc tgcccacact ccggtgtgaa ctggtccgag taatagatct
    16921 tgccccggcc gtccacgcac ttgtacgtga ccgggccgga accacccggc gtgggtttct
    16981 gctgcgccag tgccgggacg gcgagggccg tggcgatggt caggctcacc agcgaacgaa
    17041 tcactttcat gcggataccc cgtattcttt gcggtagcgt tcgactgcct gcaactgccg
    17101 tgccatatcc gcgcgcgccc gcaggaacac ggtaaggtcc gcgagcgtgg cgatgctgat
    17161 gaccgggatg ccgaactggc tgcgcacctc ctgggcggcg gactgcgtgc cgctgccgcg
    17221 ctccatccgg tcgagcgcaa tgatcacgcc gcagggcgcc gcgccggctg cgcgaatgat
    17281 ctgcaccgac tcgcgcaccg aagtccctgc ggaaatgaca tcgtccacga ccagcacctg
    17341 cccggcgagc ggtgcgccga cggtcatgcc gccctcgcca tgatccttgg cctccttgcg
    17401 attgaagcag aacggcaaat ttcgcccaat ttcggcaagc gcaacggcgg tggcggaaac
    17461 cagcgggatg cccttgtaag ccggaccgaa cagcatgtcg aaggtagcat cggcggcgac
    17521 aatggctttc gcgtagaatt gcccgagcct tcgcagcgcc tcgccgtcgt tgaacaggcc
    17581 ggcgttgaag aaatagggcg acagacgccc ggccttggtc ttgaactcgc cgaagcagag
    17641 aacaccgcgg gccaaggcga attcaataaa ctcctgcctg aaatttttca ttgcccgacg
    17701 tgcgaatcat caccctcaat ctaaacggca tacgttcggc agtcaacaaa ggctttctcg
    17761 agtggatgtc cgcacaggac gccgacgcga tctgcgtcca ggagatcaag gcgcaggacc
    17821 gcgacctcgt agaggcgatc cgcaatcccg acggataccg cggctacttt cattgcgccg
    17881 atcggcccgg ctacagcggt gtcggcatct attgccgcaa aaaaccggat cgcgtgatcg
    17941 agggcctcgg cattcccgat atcgatgccg aaggccgtta cctcgaggcg cagttcggca
    18001 acctcagcat cgtgtcggtg tacgtgccct cgggttccag ctcggcggaa cgcctggcga
    18061 tcaagttcag cttcatggag cgtttcattg cgcagttgca caggcttgcg cagagcggcc
    18121 gtgaagtcat actctgcggc gactggaatg ttgcgcacaa ggagatcgac ctcaagaact
    18181 ggcgctccaa ccagaagaac tccggcttcc tgccagagga gcgcgagtgg ttttcggggg
    18241 tgctcgacaa gctcgggtac gtggacgtgt tccgccgcct caaccaggaa ccggagcaat
    18301 acacctggtg gtccaaccgc ggccaggcct gggccaagaa cgtgggatgg cgcatcgatt
    18361 atcagctcgc cacacccggc atcgccgcca aagccagacg cgccgcaatc tacaaggcgc
    18421 agcgtttcag cgaccacgca cctcttatca tcgactatga ctacccgctg gcttagcgac
    18481 ccgcgcgcgc gtgcgcaggc gtccgcggcc cgcatgctgt tgctcggctt ctcgggtaat
    18541 tgggaagcgt aggcgtgacg cgcaaaaccg cttctcgccg gtgggcgaag cgcaaaaaga
    18601 ggggcatgga tcagaacact acgaccgcgc tacgcgactg gcgcagcagg ctgccggaag
    18661 gcgtacgccc ctattttgag tccgcgcccc tcgcggcatt gttcttgggc atctcctccg
    18721 gcttcggctt cgcgatgatc ggcgcgactc tgaccacgcg cctcgcacag cacggcatca
    18781 ccaagagcgc ggtgaccgct ttcgcgctca ctgtcctcgc ctacaatttc aagttcctat
    18841 gggcaccgat cgtcgacaac gtccggctgc cggtgatcgg caggttcgga caacggcgca
    18901 gctggctgtg gctggtcggc gtgctggtga tggccgccgt cgcatttctc ggaatcgccg
    18961 atccggacga agcgctttat atcgtcgcga tcgccgcgat taccgtgggc gttgccggcg
    19021 ccacgttcga catcatcatc gacgcctacc gcatcgagct gctcgagccg cgccaactcg
    19081 gcgtcggctc cggcatgtcg cagtacggct ggcgcatcgg cgccgcggcg gccggcgccc
    19141 tggcactggt catcgccgcc cgtctcggct ggagcgctgc ctatttcgcc tgcgcggtgt
    19201 tcgcgctccc ggggatgctg gtcgggctgg taatgggcga gcccgagcgg caccgcgagc
    19261 cggccaagcc gcgcggcgtc gtcgaggcta tcgtcgccta tttcagcccg ttgctcgagt
    19321 tcctcaagcg ccagggggcg ctggtggtgc tgctgttcgt gctcatccac aagatcggcg
    19381 acactctcgc caaccttacg ttcaggctgc tgttcaagga cctcggcttc agcaacgacg
    19441 aggtggccta ctacgacgtc ggcgtcggct tcatcgcact gctgatcggc attttcgccg
    19501 gcggcattct gtacgcgcgc ttgggcatga agcgcgcggt cctggtcagc ctgatcctga
    19561 tggcggtgtc gaatttcagc ttcgccgcac tggcggcggt cggccatacc aacaccggca
    19621 tggcggcggc gatcggcttc gagaatttcg cgagcggcat gggcggcgtg acggtggtcg
    19681 cctacctctc cgcgctgtgc aacctgcgct tcacggcaac ccaatacgcc ctgctctccg
    19741 cgctggcaag catcgccggc cgcttcctca ccgggacgag cgccggcgcg ctgatcgagg
    19801 cgatcggcta cgtgaacttc tatctgctca ccaccgtcat cgcgttcccg ggcgtgatcc
    19861 tgttctggtt catgatccgc tcggggctcg ccgacctgtc gatcggctcg gcaggcaagg
    19921 agggcaaagt agacgcgcgc gttgacaagg cggctgatgc ttaggttcat cgccgggtgg
    19981 tgcggcctgg cggtggatac attcggctac caggcgttat ttgccggctc ggcgtgccgg
    20041 catggctggc tgcccgctcc catgtcggcg gctcggcgcc cagggaaagc tggtaaccct
    20101 acgcgcgcga acccgcgtcg aagcgaaaga ccgcgagcgc gcccagcagg ttgggcaata
    20161 cgcttaccgg cttggcgccg gccatcacca ggcgttcgag aatgcgcacg ccgtgggttc
    20221 tgcagtaaac ctggaaatcc ctgatagtga aaaggtggat attcggggtg tcgtaccact
    20281 cgtaaggcag ttccttcgac accggcatgc gtccagccag aatctgcagc cggtgccgcc
    20341 agtagccgaa gttcggaaac gtgacgattc cttcgcgtcc gacccgcagc atgtccttga
    20401 ggatgcgttc ggtgtggcgc accgcctgca gcgtctgcga caggatcacg taatcgaagg
    20461 aattcgactc gaaccccgac agccccgact ccaggtcgtt ctgcagcacg ttcacgccgt
    20521 tgcgcacgca ggccagcacc ctggcatcgt cgatctcgat cccgtagccg gtcacgccgc
    20581 gcgcctgctt taaatggcgc agcagcgatc cgtcgccgca accgaggtcg agcacctgcg
    20641 catttggctt gatccacttt gcgatcgcgg caaagtcgga acggccggac cgcgtcccag
    20701 gggctgcaga cgcgctcatg tcccggggcc ctgcaggacg atgttgccga agtaggtctg
    20761 gatcaggtta tggtagcgcg gatcgtccat caggaaggcg tcgtggccgt gcggcgcatc
    20821 gatctccgcg tagctcacgc ccaggcggtt gtccaccagc gccttgatga tctcgcgcga
    20881 acgcgccggc gaaaaacgcc agtccgaagt gaacgacacc aggaggaagc ccgccttcgc
    20941 gggtttcaag gttgcagaca ggtcgccgcc gcaggctgca gccgggtcga agtagtcgag
    21001 cgccttggtg atgcgcaggt aagtgttggc gtcgaagcgt tccgcaaact tgtcggcctg
    21061 gtggcgcagg taagactcga tctggaattc gacgtcgaag ttgaacttga gcgtgtcttc
    21121 ccgcaattcg cggccgaatt tctccgccat cgtgtcgtcg gacaggtagg tgatgtgtcc
    21181 gatcatgcgt gcgatgcgaa gcccccgcct cggcaccact ccgtacgagt agtaattgcc
    21241 gccgtggaaa tccggatcgg tggtgatcgc ctggcgcgcc acctcgttga aggcgatgtt
    21301 ctgcgccgaa agtttgggtg cggcggcaat cacgatggaa tggcgcacgc gctccggctg
    21361 gctgatggcc cattgcagcg cctgcatcgc gccgaggctc ccgcccatca ccgcggcgaa
    21421 gcgctcgatg cccagccggg tggccagccg tgcctgcgcc tgcacccagt cgtccaccgt
    21481 caccaccgga aaatcggcac cccatggcct gccggtcgcg ggattgatgc tcgaaggtcc
    21541 ggtggagccg tgacagccgc ccaggttatt gacgccaatg acgaagaagc ggttggtgtc
    21601 caccggcttt cccggcccga tcaggttgtc ccaccacccg acgttctccg gatcgtcggc
    21661 atagacccct gcgacgtggt gcgaggcgtt cagtgcgtgg caaaccagca ctgcattgct
    21721 gcgcgcggcg ttgagcgcgc cgtaggtttc gtacgccaga tcgtacgaat ccaggcgtgc
    21781 gccgctcttg agctcgatcg gctcatcgaa gtgcgcgagt ttcgggctga ctacgccgac
    21841 ggaaccttcg ggtggcatgg aaatgcaagg gggcttgttt gcgcggatta tatccgccgg
    21901 ccgttatgtc gcgggcttca ccagcgacaa ccgccgcagc atttgctgga ccgcgtctgt
    21961 cttcatgtcg cggaacagca gcagttcctc cgattccttg gcgagcacca cggtatcgtc
    22021 gtaggtcatg tcgcggcgca gaacgatttc tcccggcagc gccaggtcga cgccggcgcg
    22081 attggtcagc cggtaagcga tgcgataaag cagttcgaac tcccgcacct tgccgccgct
    22141 ggacaacgac aggatgtgct tttcctgcga ttctccgacg atttggacca ccgcttcggc
    22201 ttggtcgcgg ctttcgacga tacgggtctt gcttccggca cggatggcac gttccagatc
    22261 attggcaaaa gccgagaacc ccggcgcctc gacgcgaacg gtctggaacg ggatggcggc
    22321 ctgccctcgc aattggaagc cgcaggcgga gagcgtcatc gcgacaagga aagtcgcgat
    22381 gaccagggac gagggacgag ggacgagggg cgagggaaaa tctcgcaaga atctgttcat
    22441 cgccttcatc gcgctccagg agcgctggca cacgtcggtt ttcattcgcc cttcatccct
    22501 cacccctcgt cccccgtccc tcgtccctac acaacaatat tcaccagccg tccgggcacc
    22561 accaccactt ttttgaccgt ctgaccggca atgtgtttct gcgcgttcgg gttttccacc
    22621 gccagccgct cgatggcggc gcggtccgcg gtgcgcgcca cccggatgtt gccgcgcagc
    22681 ttgccgttga cctgcagcac caggtcgatt tcgtcctgca ctagcgcggc ctcatccact
    22741 gccggccagg gcgcatcgat gatgtcggcg ccgtaaccca gctccttcca caacacacag
    22801 gtgatgtgtg gtgtcaccgg gtaaagcact cgcaacagga tatccagcgc ctccgaggag
    22861 gcctgagaca ccgcatttac gctcccaagt ccttgtcctc ccgagaatcc ttcaagcaaa
    22921 ttgagcatct tcatgcaggc ggatacgacg gtgttgtact gcattcgttc gtagtcatag
    22981 gacgcttgct tcaatgacag gtggattcct ctacgaaaat cgcgtatcgc tgatggctgt
    23041 gaagaccaat ccgtgattga aataaaagcg ctgctcaaat taaactttgt gttgtccttt
    23101 ccccgtccgg cagcgaacaa ccaaagtctc cgcagaaacc ggctggcacc ttcaacgccg
    23161 tcgtcggacc actcaagggt ttgctccggt ggactcgcga acatgacgaa caggcgtgct
    23221 gtgtcggcac cgtattgatc gatcaacgcc tgcggatcca cgccgttgtt cttcgatttt
    23281 gacatagtgc caataccgcc cgattccagc ggcttcccgt cctctttcag caccgctgcc
    23341 ttgcgcttgc cttggtcgtc gaattgcact tccacatccg cgggattgaa gtactgcgcc
    23401 cggccgccgt cggtcttgcg gaagaaaatc tcgttgagca ccatgccctg ggtgagcagc
    23461 cgggtgaacg gctcgtccag cttcaccagc ccgaagtcgc gcattacctt ggtccagaag
    23521 cgcgagtaca gcaggtgcag aatcgcgtgc tcgatgccgc cgatgtactg gtccaccggc
    23581 agccagtagt tcacccgctc gtccaccatc ctttcgtgct gatcggcgca ggcgaagcgc
    23641 aggaagtacc aggacgaatc cacgaaggta tccatggtgt cggtctcgcg cttggccggt
    23701 ttgccgcact tcggacaggc ggtgttgagg aaatccggcc gcttgttgag cggattgccg
    23761 ctgccgtccg gcacgcagtc ctcaggcagc accaccggca gctgatcgtc gggcacgggc
    23821 acgtcgccgc aggaatcgca atgcacgatc gggatcgggc aaccccagta acgctggcgc
    23881 gaaattcccc agtcgcgcag gcgccaggtc acctgttttt cgccaaggcc tttcgccttg
    23941 aggtccgccg cgatcatgtc caccgactgc tgatacggca tgccgtcgta cctggcggag
    24001 ttcacgcaca cgccgtagtc agcgtaccag ggctgccacg cgtccagcga atacggccgg
    24061 ccctcgacgt cgatcacctg cttgatccgc agaccgtact tcttggcaaa ggcgaaatcg
    24121 cgctcgtcgt gcccgggcac acccatcacc gcaccttcgc cataactcat cagcacgtaa
    24181 ttgccgaccc acacctcgat attctctccg gtcagcggat gctccacaaa tatcccggtg
    24241 ggcatgcctt ttttttcctg ggtggcgatc tcggcttcct gcacgccgcc gcgcttacac
    24301 tcctcgacga attcggcgag cttgggattg cgtctcgccg cctcggcggc cagcgcatgc
    24361 tccgcggcga ccgcgacgaa agtcacgccc atgatggtgt cggcgcgagt ggtgaacacc
    24421 cagagctttc cggccttgcc gccgagcacg tacgggaagg ccagccgcac gccgaagctc
    24481 ttgccgatcc agttggcctg catggttttc acgcgctcgg gccagccggg aagccggtcg
    24541 agcgaggaca gcagctcctc cgcgtagcgc gtgatcgcca agtaatacat cgggatttct
    24601 cgcttttcca cgagcgcgcc ggtgcgccag ccgcggccgt cgatcacctg ctcgttggcg
    24661 agcacggtct ggtccaccgg atcccagttc accgtgccgg tctttttgta ggccagccgc
    24721 ttctccagca tgcgcaggaa cagccactgg ttccagcgat agtagtccgg ccggcaagtc
    24781 gcgagctcgc gttcccagtc aatagcgaag cccagcgact tgagttgctt cttcatgtag
    24841 gcgatgttgt cgtaagtcca cttcgccggc ggcacgttgt tggccatggc cgcgttctcg
    24901 gcgggcagcc cgaatgcgtc ccagcccatc ggctggagca cgttgtagcc gcgcatgcgg
    24961 tagtagcgcg acagcacatc gccgatggtg taattgcgca cgtggcccat gtgcagcttc
    25021 cccgacggat aggggaacat cgacaggcag tagtacttgg gcttgtgcga ggcatcgacc
    25081 gcgcggaagg atttcttgtc cgcccactct tgctggacct gctgctcgat ggcggcgggc
    25141 tgatattgcg gctgcatgat gaatgaaacc aaagcctgac aaaggcggat tatagcgttc
    25201 gggcacgcat ccgttgggcg tttcaggccg tatctgtcgg cataaacatg gcaccggaaa
    25261 ggggactcat tatgctcatt cgcgcaggga tcgccgctct tttgatagcc gccgccatgc
    25321 cgttgcaggc ggctcccgac gccatggtcg aaggcgtcca gttaccggcc tggctgatcc
    25381 gcgacggcca gcgtgagccg ctcaccgccg gcaccgaact caaggcgcgt gacgaaatcg
    25441 ccaccgggtc caattcgcgc ctgctgctgc ggcttgggga cggcagtctg gtcaagctgg
    25501 gcgagaacgg gtggctgcag attgccgaac tggcgcaacg ccaggcgaag aatttcctca
    25561 gcgcgactct caaagtgttc gccggcgctt tccgttacac caccgcgccg gagccggccg
    25621 cggatagcga agtgacggtc cagttcacca ccctcatcgc caggttcaac gccgccgaca
    25681 tttggggcaa gaaactcagc gaccatcagg tgctggtgct catcgacggg aaggttagcg
    25741 tcgcccgcgt tgccgaaagt tcggcgagcc cgccgctgga catgaaagac gcgctcactt
    25801 tcctgcaggc gccgagaagc ggcgccggga agatcgagcc cgtccagatc gaacaactca
    25861 agatctgggc cgccgaaacc gaaatctcgg agggccaggg cgcaattcgc cggggcggcc
    25921 tctggaaact caatctcgcc aagtatgcga atcaaatcca ggcgctgtcg ctgtacgaca
    25981 gcctccgccg cgaaggttac ccggcgctca tccagcaaga gcctcgcgaa gaaggcccgg
    26041 cttacctggt gcgcatcgcc ggctttccca gcgagctgga agccaacctg ctcggcgcac
    26101 ggctgaagca gatcaatccc cggctggagc cgatcgcctc actgcagtaa ttttttgtaa
    26161 ccgcggccac caccaaaccg gcggccgagc ggaagacctt cgaaggcatc ttcgagcgcg
    26221 agcgcgtcaa tgcggcgcgg atcgaagaag acagctttca cattcgccga tgccaatgga
    26281 gtgaactttc cccacctgct ggcgatgatc gacagggaga cgaaaaacta gagacacgcg
    26341 acctgttggt gcgaggtcgc ctttccctca ccttctccgc ccgcttacgg ggggagaggg
    26401 ccggggttag gtgggtataa ttcggcacca aaacgttcct cccgccgcgt cccgaatgtc
    26461 gattctcgaa ggtctgaatc cccagcagct cgaagccgtc acgctcccgc accagtccgc
    26521 gctgatcctt gccggcgcgg gctcgggaaa gacccgcgtg ctcaccaccc gcatcgcgtg
    26581 gctgattcag accggccaga tcgggccgct gagtgtcatg gcggtgacct tcaccaataa
    26641 ggctgcgcgc gaaatgctca cccgcatttc ggcgatgctg ccgatcaaca cgcgcggcat
    26701 gtgggtcggc actttccacg ggctgtgcaa ccgcatcctg cgcgcgcact accgtgacgc
    26761 cggattgccg cagaccttcc agatcctcga ttccgccgac caactggcgg tgatcaagcg
    26821 ggtgatgaag gcgatgaacg tggacgacga gaaatacccg ccacggcagg cgcagaactt
    26881 catcaactcg gccaaggagc agggcttgcg ggcggcgcaa gtggacgcgc ccgacgactt
    26941 ctcgcggcgg ctgctcactg tctacgaagc cttcgaggcg cagtgcaatc gcgaaggcgt
    27001 ggtggatttc gccgaactgc tgctgcgttg ctacgaactg ctgggcagga acgagctgct
    27061 gcgagaccat taccgcgggc gtttcaggca catcctggtg gacgagttcc aggacaccaa
    27121 ccggcttcag taccactggc tgaagctgct ggccggcccg aacaacacca ttttcgccgt
    27181 cggcgacgac gatcagtcca tctatgcgtt ccgtggagcg cacgccggca acatgctcga
    27241 tctggagagg gacttcggta tcgagaaagt catcaagctg gagcagaact accgcagcca
    27301 cggcaacatc ctcgatgccg ccaatgcgct gatccgccac aaccgcaccc ggctgggcaa
    27361 gaacctgtgg acctcggaag gcggcggcga gccgctgcgg gtgtaccagg ccatgaccga
    27421 catcgaggaa gccgcctaca tcgtcgacga ggtgcgccag cttcacaatg agggcgtggc
    27481 attgtccgac atgacgctgc tgtaccgctc caacgcgcag tcccgcgtgc tcgagcacgc
    27541 gttgttcagt gccgggattt cttatcgcgt gtacgggggg ctgcgctttt tcgagcgcca
    27601 ggagatcaag cacgcgctcg cctacctgcg cctcaccgca aatcccgacg acgacggcgc
    27661 tttcctgcgg gtggtcaact ttcccacgcg cggaatcggc aaccgcagcc tcgagcaact
    27721 gcaggaggcc gccgcagggc gcggcatctc gctgtggcag gccgcctgcg ccaatacgcc
    27781 gagcggcaag gccggcgcca gcatcgccgc tttcatccag ttgatcgagg cgatgcgcaa
    27841 agcgtgcgag aacctgccgc tgcccgagat cgtcgaccac gtggttgcga ccagcggcct
    27901 gattgcgcag taccagaccg agaaggaggg cgcggaccgg atcgaaaacc tcaatgaact
    27961 gaccaacgcg gcggcggcgt tcgtcggcca ggacggcccg attacgttga tcagcgcgga
    28021 aggcgaaatc aggtcggaag gtctggaccc gctgaatgcg ttcctggcgc acgcggcgct
    28081 cgaagccggc gaacaccagg cgggcgaggg cgcggatgca ttgcagttga tgacggtgca
    28141 ctcggccaag ggcctggagt ttcacacggt gttcatcagc ggcctggagg aaggcttgtt
    28201 cccgcacgag aacagcgttt cggagcaaga cggcctggag gaggaacggc ggttgatgta
    28261 cgtcgcgctc acccgcgcgc gccgcaggct ttacctcacc cacgcgcaga cgcgcatgct
    28321 gcacggccag acccgctata acgtgccctc gcgctttttc gacgagattc ccgacgagtt
    28381 gctgaaacga atcaacacca cgaagttctc cggcttcgtc gccgccaagg gatatgaagc
    28441 cggctccaaa gccagggcgc cgatggtcga gaccggcgca tcaggctggc gcatcggtca
    28501 gagcgtggtg catccgaagt tcggcgccgg ggtgatcgtc aacgccgagg gccgcggctc
    28561 ggacgcgcgg gtccaggtca atttccgcga cgcgggactg aagtggctcg cgctcgacta
    28621 cgccaagctc gttgcagcgt aatctcaagc gtttcagttc gcctggccgg atacgaacac
    28681 atcgcgatag ctggtgtaga tggtcagcag catcaccggg atccacagga tcagaccgag
    28741 gccgaagggg atcacggcga acagcagcag gacggaactg acgatgctgt agagcaacag
    28801 gggcagggcg ttgcgaaagc aggccagcag gctggtccac aacgcctgga agccgggctg
    28861 gtcgtcgagg atcaccaacg ccggcgcgaa ccacaccgcc atcgccagcg gcatgaacaa
    28921 taccgttccc accagcatcg cctggaaaat gcgcgttgcg ccctctggcg tgagcgccga
    28981 ggcatcgagc gcagcgatcc cggccttcac cgattcctgg aactccggac caccgaccgt
    29041 cagcatcacg ccggagatca ccacctgtcc caccagatac accccgccga ccgtcaccag
    29101 atgcgcggcg ttgtggcgaa agccgcggaa caggtgtgcg atctcgatgt cctgcccggc
    29161 ctcgaggtcc tttgcggccg ccatgatccc gcccaggaat accggggtga gcaggtacag
    29221 cacgtaagtg ccgattaccg gcagcatggc cagccccacg ccgatcagca tcagcgccat
    29281 attgagcacc acccagatca ccgggttctt gcggaacaat tgaaaggcgg cggtgatcca
    29341 ctgccagccg cggccgggcg cgacactccg gatgttcatg aagattgccc cgggtttata
    29401 cggtctgccg ctgcggccgg tccgcggcct tgcggtgatt gagcagaatg cggcggaaac
    29461 gctccggatc gtgggcgtgg gtgagttcgc ccggacgcgg cagatggaaa tcgtaaagcc
    29521 gcgaaaccca gaatcgcagc gcgcccgcgc gcagcatcca tggccaggca tcgcgctcct
    29581 gatcggtaaa cggccgcacc gcggtgtacg ccttcaacag cgcctgggtg cgtgccgggt
    29641 cgagcgtcgc gtccgggttg gtacaccagt cgttcaccgt gatccccacg tcgtagatga
    29701 aacaatcgac gcaggcaaaa tagaaatcga tgaagccacc gacgtggggg ccatgatttt
    29761 gatcgtgctg gatcagcacg ttgtcgcgga acaggtcggc atggaccacg ccgcgcggca
    29821 ggccttctcg cacctgctgc gcctgaaagc ggatttccga ctccagcagt tcagcgtcct
    29881 cggcggtgag gaacggatag atctccggcg cgcaggcggt ccaccacttc tcgccccgaa
    29941 ggttgtcgag atggcttttg aatgtccggc cggcgaggtg catgtccgcc agtaccccgc
    30001 cgacttgcgc gcaatgctcg ggcgttggtt cggtgacctg ttggccttcg agacaggtga
    30061 cgatcgccgc cggcttgccg ttgagggtgc ccagcagttc gttctgcagg ttggcgatcg
    30121 gcttcgggca gggcaggccg tggctggaaa gatgcgccat caggttcaga taaaacggca
    30181 gttcctggcg ggtgagcttc tcgaacagcg tcagcacgaa cttgccgtgc gtggtggtga
    30241 cgaaataatt ggtgttctcg atcccggaca ggatgccctg tagatcggtg agcacgccga
    30301 tcgagtaatt gcggagccag gccgagagct gctccggcgt aacggtggtg aagacggaca
    30361 tgaacgggct acagattcag ttgcgcttcg cgctccgcgg cggacggttc gaagccgcgc
    30421 ttctcgtaat gactgaagat cgcctcgact acttgcctgg gttcgtcgat gaccttgatc
    30481 aggtccatgt cggccgcatc gatcatgcct tcactgatca tgcgatcacg catccagtcg
    30541 atcaggccgc gccagtagga ttcctgcacc aggatgatcg gaatgcggcg gatcttctgc
    30601 gtctgcacca gcgtcagcgc ctccatcagt tcgtccatgg tcccgaatcc gccaggcacg
    30661 actacgtatg cggatgcgaa tttcacgaac atgaccttgc gcgcgaaaaa gtgacggaag
    30721 gtctggctga cgtcctggta ctggttggag atctgctcgt gcggcaactg aatgttgagc
    30781 ccgaccgaaa gcgagcggcc gaagaaggcg cccttgttgg ccgcctccat cacccccggg
    30841 ccgccgccgg agatgacgct gaagccggcg tcggacaact ggcgcgcgat ctcctcggtg
    30901 aggcggtagt agggatggcc ggcggggatc cgcgcgctgc cgaatatgct gaccgcgggc
    30961 cggacgttgg cgaggcgttc ggtggcctcg acgaattctg ccataatccc gaacacccgc
    31021 caggattcgc gcgccgacca cgctttctcg accaactctg gggcgagcgg taccggaacc
    31081 ttctgcttgt cgctcatgct ggattttccg cgctgatcat cgatgaaaac gctcttgctg
    31141 gtcgacggtt cgtcctacct ctaccgcgca ttccacgcgc ttcccgacct gcgcaacagg
    31201 aacaacgaac cgaccggcgc cgtgtacggc gtgctcaaca tgctgcgccg gctgcgtaag
    31261 gaaactgcgg cggattatat cgcgtgcgta ttcgacgcaa aaggaaagac cttccgcgac
    31321 gactggtacg ccgagtacaa ggccaaccgc ccgtccatgc cggaggagct ggcggcgcag
    31381 atcgaacctc tgcaccggag cgtgcgcgcg atgggctggc cgctgctgat ggtcgagggc
    31441 gtcgaagccg acgacgtcat cggcacgctg gcaaagcagg ccgaggcgaa atgtgtgcaa
    31501 accgtcatat ccaccggcga caaggacatt acccagctgg tcagcccgaa ggtcaggctg
    31561 gtgaacacca tgtccaacga gaacctcgac gaaaagggcg tcgaggagaa attcggcgtc
    31621 aaaccggagc agatggtgga ctatctcacg ctggtcggcg acagcgtgga caacgtcccg
    31681 ggcgtggaga aggtcgggcc caaaaccgca gtgaaatggc tgaagcagta cgggacgctg
    31741 gaccaggtgg tcgcccacgc caacgagatc gccggcgtgg tcggcgagaa cttgcgcaag
    31801 gcgctggact ggctgccgca ggcgaagaaa ctgctcacca tcaagtgcga cgtcgagctg
    31861 ccggtgaagc tcgaagacct caccccgcag ccgcaggaca cggcgaaact ttcagaactc
    31921 ttcgatcgcc tcgaattcaa gagctggttg aaggagcttc aggcacgagg cccggggcac
    31981 gaggcacgag gtgaagccga gtctccggct tcatcgcctc acgccctcga ttccggccct
    32041 gacgccgcac ccctcacgcc tcacgcctca cctgcgcgca attacgaaac cattctgact
    32101 gatgaacaat tgcgcgcctg gctctcccgc atcgaagccg ccgaactggt ctcgatcgac
    32161 actgaaacca ccggcctcga tcccatgacc gcgagactgg tcggcatctc ggcgtcggtc
    32221 gagccccatc acgccgcgta cataccggtc gggcacaact acgccggcgc gccggagcag
    32281 ctcgcgctgg atcgggtgct cgctgtgctg aaggactgga tgcagagccc gaaacatttc
    32341 aaggtcggtc agaacctgaa gtacgacaag cacgtgtttg ccaatcacgg cgtcgatctc
    32401 gcgggcatcg tgcacgacac actgttgcag tcctacgtgc tggaaagcca ccagtcgcac
    32461 gatatggaca gcctcgcggc ccgacacctg ggcatcaaga ccatcagcta cgacgaagtc
    32521 accggcaagg gcgcgagccg cattcccttc gagcaagtcg ccctggagcg cgccaccgag
    32581 tactcgggcg aagacgcgga cgtgacg
//
LOCUS       DASQOP010000143         2048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_58254, whole genome shotgun sequence.
ACCESSION   DASQOP010000143 DASQOP010000000
VERSION     DASQOP010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58254"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VE030_05920"
     CDS             <1..455
                     /locus_tag="VE030_05920"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZF22986.1"
                     /translation="VVLSTVGVGDYFGELVLDGGQRSASIMTLEPCRFFVIPRGDIEG
                     LIDRNPAFARHLIRMLIGKVRSLTQKVLDLALKDVYGRFAKFIDESAIEQKGTRVVPE
                     RLTQHDIAARIGGSREMVNRIVKDLTAGGYISVDAKQITVHKKLPSHW"
     gene            631..861
                     /locus_tag="VE030_05925"
     CDS             631..861
                     /locus_tag="VE030_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22987.1"
                     /translation="MMNRKNLSGESAFFAVTAIAICAVLVAAAAGLLAPAAATSGVST
                     NSTATYAAPSGYFPDQFVNQAKEVEPMPVMYE"
     gene            complement(992..>2048)
                     /locus_tag="VE030_05930"
     CDS             complement(992..>2048)
                     /locus_tag="VE030_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22988.1"
                     /translation="RCLAVALLAATPWLIIWPYALYQRSPELFMQWFWVNNLGRYFGF
                     AHLGAQSEPWFYTRTLPWFAWPALPLALWGAWRLGPEGLKRPELQLPVLAVIVMLAVL
                     GASASAGTQYALPVLVPLSVLAAGAADLIPQRAAATLDWLSRVVFGLLAVALWWGWME
                     MMFRGHPPEWSWLTSHLPADFRPTFQGFAFAVAAAFTLAWILLLPRLKEWKIRPLLSW
                     TAGLTLVWGLLSTLWLPWLDNAKSYRGMFASMQRALPMDHGCLASRNLGEHERAMLEY
                     VTGIVSLRREVRPDARCDFILLQDKAADQRVPLQGGWQLVWKGSRPGDNRERFSLFGR
                     NSEEAGKLITASGTRPE"
BASE COUNT          426 a          661 c          642 g          319 t
ORIGIN      
        1 aggtggtgct cagcaccgtc ggcgtgggcg actacttcgg cgagctggtg ctcgacggag
       61 gtcagcgttc cgcttcgatc atgactctcg agccgtgccg ctttttcgtg atcccgcgcg
      121 gcgacatcga gggattgatc gatcgcaacc cggcgttcgc gcgccacctg atccgcatgc
      181 tgatcggcaa ggtgcgcagc ctcacccaga aggtcctcga cctggccctg aaggacgtct
      241 acggccgctt cgccaagttc atcgacgaga gcgcaatcga gcaaaagggc acgcgcgtag
      301 tgcccgagcg cctgacccag cacgatatcg ccgcgcgaat cggcggctcg cgcgagatgg
      361 tgaaccgcat cgtcaaggac ctcaccgcgg gcggctacat ttcggtcgac gccaagcaga
      421 tcacggttca caagaagctc ccttcgcatt ggtgaggcgc ggggcgccgc ccgcgcatcg
      481 gctcgcgccg ccgccctggc ggcacgaaat gtgcatttgg cacaaaaccc ctgtgtcagg
      541 cgacggcgag gaaggacgat caaaacccta aagtggtctc catcggacag gcgatttccg
      601 ccggcccgga aacgagacaa ggagaccacc atgatgaacc gcaaaaatct ctccggcgaa
      661 tccgcgtttt tcgccgtcac cgcgatcgcg atctgcgccg tcctggtagc ggcagcggca
      721 ggcctgctgg ccccggcggc agcgacctcc ggcgtttcga ccaacagcac cgcgacttac
      781 gccgccccca gcggctattt cccggaccag ttcgtcaatc aggccaaaga agtcgaaccg
      841 atgccggtga tgtacgagta agccagcgag cgacgcgaac atgctgggcc tggaccgccg
      901 gcggcggggg agggaataaa gcgctgccgg cgggatcggg gacaagcctg gagaacttcc
      961 aggctttttt tcgttcgaga cgttcgcgtg gtcattcggg cctggtgccc gaagccgtga
     1021 tcagcttccc cgcttcttcg gagttgcgcc cgaagagcga gaatcgctcc ctgttgtcgc
     1081 ccggtcggct ccctttccaa accagctgcc agccgccttg cagagggacg cgctgatctg
     1141 cggccttgtc ttgaagcagg atgaaatcgc atcgagcatc gggccggact tcgcgcctca
     1201 gcgagacgat tccggtcacg tactccagca tggcgcgctc gtgctcaccg agattccggc
     1261 tggcgagaca gccatgatcc atcggcaggg cgcgctgcat ggacgcaaac atcccgcggt
     1321 agctctttgc gttatcgagc cacggcagcc acagcgtcga gagcagaccc caaaccagtg
     1381 tgagtcccgc cgtccagctg aggagggggc ggattttcca ttccttcaac ctgggcagga
     1441 gcaggatcca ggccaaggtg aacgccgcgg ccacggcaaa ggcgaatccc tggaacgtgg
     1501 gcctgaaatc ggcaggcaag tgagaagtca accaggacca ttcgggtgga tgtccccgga
     1561 acatcatctc catccagccc caccatagcg ccacggcgag caaaccgaac acgacgcggg
     1621 acagccagtc gagtgttgcg gcggcgcgct gcggaatcaa gtcggccgca cccgccgcaa
     1681 gcaccgacag tggcaccagg accggcaacg catattgcgt acctgcagaa gcagaagcgc
     1741 cgagaactgc aagcatcacg atgacggcaa gcaccggcag ttgcagttcc ggccgtttca
     1801 atccttccgg cccgagccgc cacgcgcccc acagcgccaa gggcagcgcc ggccaggcaa
     1861 accacggcaa agttcgggtg taaaaccaag gttcggattg cgcccccagg tgagcgaatc
     1921 cgaaataacg cccgagattg ttgacccaga accattgcat gaacagctcg ggggatctct
     1981 ggtagagagc gtagggccag atgatcagcc aaggcgttgc ggcgaggagg gcaaccgcaa
     2041 ggcagcgc
//
LOCUS       DASQOP010000144         2157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2674289, whole genome shotgun sequence.
ACCESSION   DASQOP010000144 DASQOP010000000
VERSION     DASQOP010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2674289"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VE030_05935"
     CDS             <1..512
                     /locus_tag="VE030_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HZF22989.1"
                     /translation="LVNTHPFMRELWGRYWIFAHNGTLENFHPDFGNHYRPVGTTDSE
                     QALCYLLDTLRLRYPEGQPPLPELYGTMATLSAEIARYGRFNYILSNGDYLFAHCTDN
                     LTYVLRHAPFATAHLKDQDIEVDFSEVTTPMDRIAVIATEPLTDNETWTKFAPGKLLA
                     FQDGAPLSL"
     gene            complement(645..1544)
                     /gene="gluQRS"
                     /locus_tag="VE030_05940"
     CDS             complement(645..1544)
                     /gene="gluQRS"
                     /locus_tag="VE030_05940"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HZF22990.1"
                     /translation="MTIYRGRFAPTPSGPLHFGSMVAAVGSFLDARSRGGIWLVRIDD
                     LDPPRTVPGAADAILECLDAYGMHWDGAVVYQNTRFDAYHAALHQLRQSGRTYPCACS
                     RKEISDAGLAGVDGAVYPGTCRNGLAAGREARALRLHTDGAVVEFDDGLQGHVRQALP
                     VEIGDFVVYRADHVFAYHLACAVDDAEQGITDVVRGADLIASTPRQIYLQKLLGLPTP
                     AYLHLPAAVNGQGEKLSKQTRAPAVDLSDPAATLDRVLRLLGHAPPAELASAGIAGLW
                     QWAVKNWQRERLPRARTIAVTKS"
     gene            complement(1765..2109)
                     /locus_tag="VE030_05945"
     CDS             complement(1765..2109)
                     /locus_tag="VE030_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HZF22991.1"
                     /translation="MRRLLQVISSGALVALIFLCLAWELWLAPLRVGGSSLAFKALPL
                     LLPLFGVLRGKRYTYQWSSMLILAYFTEGVVRAWSERGAGQALALAEIMLSVVFFTTA
                     IFYARFTHALPR"
BASE COUNT          426 a          694 c          647 g          390 t
ORIGIN      
        1 cgctggtcaa cacccatccg ttcatgcgcg aactgtgggg acgttactgg atcttcgcgc
       61 acaacggcac gctcgagaat ttccacccgg attttggcaa ccactaccgg ccggtgggca
      121 cgaccgacag cgagcaggcg ctctgctacc tgctggacac cttgcgcctg cgctaccccg
      181 agggacagcc gccgctgccc gagctgtacg gcacgatggc cacgctgtcc gccgagatcg
      241 ccaggtacgg caggttcaac tacatcctgt ccaacggcga ttacctgttt gcgcactgca
      301 ccgacaatct tacctatgtg ctgcgccatg cgccgttcgc gaccgcgcat ctgaaggatc
      361 aggacatcga agtggatttc agcgaagtca cgaccccgat ggaccgcatt gcggtgatcg
      421 ccaccgagcc attgaccgac aacgagactt ggacgaagtt tgcccccggc aagttgctgg
      481 cgttccaaga cggcgcgccg ctatctcttt aactatcggc cggccaaagg ccggcgaaaa
      541 gagcgttcga gagaaactca tcccctatcg attttctttg cgtacctttg cgtctttgcg
      601 gtgagattcg ggatttacgg cgcgatttgt tctctggcac ggcgtcacga tttggtgacc
      661 gcgatggtcc gggcgcgcgg cagccgttcg cgctgccagt tcttgaccgc ccactgccac
      721 agtcccgcaa tgcctgcgct ggcgagttcc gcgggcggtg cgtgacccag taagcgcagc
      781 acgcgatcga gcgtggcagc cggatcagat aggtccactg caggcgcgcg cgtctgcttc
      841 gagagttttt ctccctggcc attcaccgcc gccggcaggt gcagataagc cggcgttggc
      901 agccccaata atttctgcag atagatttgc cgtggcgtcg aggcaatgag gtcggcaccg
      961 cgcacgacat cggtgatgcc ttgctcggca tcgtccacgg cgcaggcgag gtggtaggcg
     1021 aacacatggt ccgcacggta aaccacgaaa tcgccgattt cgaccggcaa agcctggcgc
     1081 acgtgcccct gcaatccgtc gtcgaattcg accaccgcgc catcggtgtg tagccgcagt
     1141 gcgcgcgctt cgcgcccggc tgccagccca ttgcggcaag tgccggggta aaccgcacca
     1201 tccacgccgg cgaggcccgc atcgctgatc tctttgcggc tgcaggcgca gggatacgtg
     1261 cggcccgatt gccgcaactg atgcaacgcc gcgtgatacg cgtcgaagcg tgtgttttgg
     1321 tacaccactg cgccatccca atgcatgccg tatgcatcca ggcattccag gatcgcgtcg
     1381 gcggcccccg gaacggtgcg cggcggatca aggtcgtcga tgcgcaccag ccagatgccg
     1441 ccgcgcgacc gggcgtcgag gaaactgccc accgccgcca ccatcgaacc gaagtgcagc
     1501 ggtcccgacg gcgtcggcgc aaatctccca cggtagattg tcacggaagt cttgttcgag
     1561 aataataaat cttcaccgca aaggcgcaaa gacgcaaagg aaacgcgaag gaaaaaagat
     1621 cggtatgatt cttgatggca gaattcgacg gaggtgcagc aggtgcgaat ggctttccgg
     1681 attcgaagtg ctcaatactc ccttctcttc cttgttcttc ttggcgttcc ctttgcgtct
     1741 tcgcgccttt gcggttaaag ggacttatcg cggcagcgcg tgagtgaagc gggcgtagaa
     1801 gatcgctgtg gtgaagaaga caaccgacag catgatctcg gccagcgcca acgcctggcc
     1861 ggcaccgcgt tcgctccagg cgcgcaccac gccctcggtg aagtaggcga ggatcagcat
     1921 cgacgaccac tggtaggtat agcgtttccc ccgcaggacg ccgaacaacg gcaacagcag
     1981 cggcagcgcc ttgaaagcca gcgacgaacc gcccacgcgc agcggcgcca gccacagttc
     2041 ccaggcgagg cacaggaaga tcagcgccac cagtgctccg ctggatatga cctgcaacaa
     2101 gcgcctcaca cgcggctcgc ctttgccaga ttcaccagtt cggcgcgggc cgcaccg
//
LOCUS       DASQOP010000145         7986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1670243, whole genome shotgun sequence.
ACCESSION   DASQOP010000145 DASQOP010000000
VERSION     DASQOP010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7986
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1670243"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="VE030_05950"
     CDS             <1..587
                     /locus_tag="VE030_05950"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZF22992.1"
                     /translation="GSGLVGRTLGVIGVGNIGKEIFRLAQPFGMRLLGCDPHVPQSAV
                     ASLNVTMVDKDKLLREADFISVNTLLDEQTRGLIGAREFALMKPNAYFINTARGPVVV
                     ESALIDALRSNRIAGAAIDVFEKEPVAPDNPLLKLDNVILAPHAICHTDECMSLLGQG
                     AFRAAVELAHGRKPHLIVNTEVLRHPAWIGKFHA"
     gene            759..1559
                     /locus_tag="VE030_05955"
     CDS             759..1559
                     /locus_tag="VE030_05955"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HZF22993.1"
                     /translation="MRTNPVKTKLLAGQPVFGSFGWEFLVPGLPQIVKAAGAEFLLLD
                     MEHAGHSYETIKDQAAACRGIDLVPMARIPTNQYHYISRALDLGIMGIMAPMVGSAEE
                     AQYIVSCTRYPPAGRRGAAFGFAADDYIGGSVADKIKIANERTLVMCLIETAEGIENV
                     EKIAAVPGVDVLWLGHFDLTNFLGIPAQFDHPKYVAAIKKMVAAASKHGKILACMTAD
                     EKWSREYWEKGFRLFSVGIDSMLLQGALKQGIGVLQTLEETGKKVRKK"
     gene            1621..2640
                     /locus_tag="VE030_05960"
     CDS             1621..2640
                     /locus_tag="VE030_05960"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZF22994.1"
                     /translation="MAQAKFRIGITRDNLKPDGKPIFDESAFKILDDPRIEYEFLPEI
                     EPELTPETAARYDALAVMLARITRKTVSGANRRLKLIARFGVGYDTVDVPACTESGVI
                     LSITPDGVRRPLATAELTLVLMLAQKVLIKDKLTREGRWQERMNHFGTGLSGRVLGSI
                     GVGNVGAELFRLAAPFGMKHLACDPYVTQESVAPLGVRLTDMESVFRESDFVVVNCPL
                     NEETRKLVGARQFSVMKPTAFFINTARGPIVDEKALLDTLANRRIAGAGLDVFEQEPT
                     PADNPLLKLDSIVVTPHHICLTDECINTIAASVFSACRDLAHARVPRNVVNQEVLGKV
                     PYFHR"
     gene            2757..3533
                     /locus_tag="VE030_05965"
     CDS             2757..3533
                     /locus_tag="VE030_05965"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HZF22995.1"
                     /translation="MRPNPLKKRLLDGASAYGLFAFEFFTPGLAQIAKEAGAECVLFD
                     MEHSGAGIDAIKQQLAYCRGLDIVPFVRVPTHQYHFVARALDAGALGIMVPMVETARQ
                     ARDIVSWTRYPPRGRRGAVLGAAHDDYSGGSMKEKFALANERCFLMLQVETEVGVENV
                     EEIAAVPDVDCICIGYADLSNFLGVPGELKHPKYLGAVKRIAAAANKHGRVLATAAPD
                     EIFAKEYAALGFRMFFFGTDAHLLQSALARRIAAMREGGR"
     gene            3607..4467
                     /locus_tag="VE030_05970"
     CDS             3607..4467
                     /locus_tag="VE030_05970"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HZF22996.1"
                     /translation="MIAVRKSDACMGAEISGVDLSRPLSDRISGAVRDALHRHHVLAF
                     RGQSLEPESFLEFASRFGRPEPHVLDQFHHPEYTDILVLSNVKKNGEPIGLADGGTYW
                     HSDYSYLQIPARATLLYSIQVPKIGGDTLFADQEQAYEELAEAMKKRIDGMVTLNVYG
                     NRDDLDVSSRTSAFAPTEEQKEKRGAALIRHPLVRRHPYTGRKALYAVSGTSFAIEGM
                     PDDEGLSLLRELAAHSTQPKYRYRMKYGVGDMVVWDNASVLHSATLTDPDDARTLFRI
                     TVKEAQRPVA"
     gene            4460..5236
                     /locus_tag="VE030_05975"
     CDS             4460..5236
                     /locus_tag="VE030_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF22997.1"
                     /translation="MPERKTPWRKNAKKGGNKYELPEGAAFRHECSMNLPVDTRIYRK
                     VLRVSLRLCAFASKSGFLGVCFTAARAAALLGVVLVAAAAWSADSMLVIENTAIEKKL
                     REQFFTDKGRFWLNNADVCNQSWLESPKVTAAAGRLRIGVLFNGRVGAQVGGQCVGGS
                     DAFNVTASGKPFFRDGRLGVEDIRIDSLSKEMYRPLLQPVFSSMVPKALDVNLKETVV
                     GLLAGRTAPYEVQINEIVATEVSADADRVNVRLQFDLRVR"
     gene            5455..6543
                     /locus_tag="VE030_05980"
     CDS             5455..6543
                     /locus_tag="VE030_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZF22998.1"
                     /translation="MLFLRDPRVLRGSLFSLFLALAAAQASPYPEPVHGDFVIRDFRF
                     AGGEVLPELKLHYFTLGNPARDTNGRVTNAVLLLHGTGGKGTNFLTDGFAGELFGAGQ
                     PLDARRFYIIVPDNLGHGKSTKPSDGLRAKFPRYGYADMVAAQRRLVTDGLGVRHLRL
                     IIGTSMGGMHAWMWAESEPDFMDAVLPMVCLPDQISGRNRMERRLMVDAIRNDPEWNG
                     GDYAKQPQGLITAFKMVIIGAGSVKQYYLEAPTQEATDRLLDRQLQQRLQGTDANDFL
                     YAWEASRDYDPGPGLERIRAAVTAVNFEDDERNPPELGIMERAIKRVARGKYVLVPAS
                     ERTRGHASFYDVGLWKEDLEKLLERSAR"
     gene            complement(6832..7497)
                     /locus_tag="VE030_05985"
     CDS             complement(6832..7497)
                     /locus_tag="VE030_05985"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZF22999.1"
                     /translation="MKNDRLQTLKEIGTGTAASKATEILADMPARPALADIFMLRMDK
                     PKFGMPFAAGSHCVQCATLALKAGMDEETVLACLLHDVGMVVMRPDHGWWGAQLVEPY
                     VSEKVAWAIRYHQALRYYPDESAGYEYPAMYVAMFGENYRPPEHIEAAYRYARNHPWY
                     MVARQITVFDDYSFDRNAPWPLEPFADIIGRHFRQPREGLGNDSSPTAHMWRTIIDPN
                     KPL"
     gene            complement(7532..7780)
                     /locus_tag="VE030_05990"
     CDS             complement(7532..7780)
                     /locus_tag="VE030_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23000.1"
                     /translation="MKLNGHARPIKTERDFKGAVSVVRTIVNQPDRETTAEKRLQSLI
                     REMEKFDDAVDDDMPSIPEEDSHGGPKRRWSDDNSDPE"
     gene            complement(7840..>7986)
                     /locus_tag="VE030_05995"
     CDS             complement(7840..>7986)
                     /locus_tag="VE030_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZF23001.1"
                     /translation="VHRPTSLLKRYATPEEVAGLVAYVCSPLSSATNGAALRVDGGVV
                     KACF"
BASE COUNT         1588 a         2373 c         2495 g         1530 t
ORIGIN      
        1 tcggcagcgg cctggtagga cgcacgctcg gcgtgatcgg cgtgggcaac atcggcaagg
       61 agatcttccg gctggcgcag cccttcggca tgcgcctgct gggttgcgat ccgcacgttc
      121 cgcaatcggc cgttgcatcg cttaacgtta ccatggtgga caaggacaag ttgttgcgcg
      181 aagcagattt tatttcggtc aatacgcttc tcgatgaaca aaccagaggc ttgatcggcg
      241 cgcgtgaatt cgcactgatg aagccgaacg catacttcat caataccgcc cgcgggccgg
      301 tggtggtgga atccgcgctt atagatgccc tcagaagcaa ccggatcgcc ggtgcggcga
      361 tcgatgtgtt cgagaaggaa ccggtggcgc cggacaaccc gctgctgaaa ctggataacg
      421 tcatcctcgc cccgcatgcg atctgccaca ccgacgagtg catgagcctg ctcggacagg
      481 gggcgttcag ggcggcggtg gagctggcgc atgggcgcaa gccgcacctg atcgtcaaca
      541 cggaagtgtt gaggcatccg gcgtggatag ggaagtttca tgcatgaagt gcaggggaga
      601 gggccgaggg gcgagggacg agggaaaacc aggaaagctc tatctggaaa gcattgtaag
      661 cagacctgtc tcgtcctagt gttttccagc ccccctctcc cctcgtccct cgcccctctt
      721 cccttgtgaa atcacctacc aacagcgagt aacgataaat gcgaaccaac ccagtaaaga
      781 caaaacttct ggctggccag ccggtgttcg gtagtttcgg ctgggagttc ctggttcccg
      841 gcctgccgca gatcgtgaaa gcagcgggag cggagttcct gctcctcgac atggaacatg
      901 ccgggcacag ttacgaaacc ataaaggacc aggcggcggc gtgccgtggc atcgacctgg
      961 tgccgatggc gcggatacca accaaccagt accactacat ttctcgcgcg ctcgacctcg
     1021 gcatcatggg gatcatggcg cccatggtcg ggtccgccga ggaggcccaa tacatcgtct
     1081 cttgcacccg ctatccgcca gcgggccggc gcggcgcggc gttcggattt gcggccgacg
     1141 attacatcgg cggaagcgtt gccgacaaga tcaagatcgc caacgagcgt actctggtga
     1201 tgtgcctgat cgaaaccgcc gagggcatcg agaacgtgga aaagattgcc gcggtaccgg
     1261 gcgtggacgt gctctggctc gggcacttcg acctgaccaa tttcctcggc atcccggcgc
     1321 agttcgacca tcccaaatac gtcgccgcca tcaagaaaat ggtcgctgcc gcaagcaagc
     1381 acggcaagat cctcgcctgc atgaccgcgg acgaaaaatg gagccgcgag tactgggaaa
     1441 aaggcttccg cctgttctcg gtcgggatag actcgatgct gctgcagggt gcgttgaagc
     1501 agggcatcgg cgtgctgcag acgctcgaag aaaccggaaa gaaagtgaga aaaaagtaag
     1561 ctgccaggaa gcggcctgcc gaaaaaaaca gagcggctgc aacagtcaag gaggagagaa
     1621 atggctcagg ccaagttccg catcggcatt acccgcgaca acctcaagcc ggacggcaag
     1681 ccgattttcg acgaatcggc cttcaagatc ctggacgatc cgcggatcga atatgaattc
     1741 cttcccgaga tcgagccgga gctcaccccg gagaccgctg caaggtacga cgcactggcg
     1801 gtcatgctgg ccaggatcac ccgcaagacc gtctccggcg cgaatcgcag gctcaagctg
     1861 atcgcacgct tcggcgtcgg ttacgacacg gtggacgtgc cggcctgtac cgagagtggc
     1921 gtgatcctca gcatcacccc ggacggcgtg cgccggccgc tggcgactgc cgagctcacg
     1981 ctggtcctga tgctggcgca gaaagtgctg atcaaggaca agctcacccg cgaagggcgc
     2041 tggcaggagc gaatgaatca tttcggcacc ggcctgagcg ggcgagtgct cggctcgatc
     2101 ggcgtaggca atgtcggcgc ggagctgttc cggttggccg ccccgttcgg gatgaagcat
     2161 ctggcctgcg atccgtatgt cacccaggaa tcggtggcgc cgctgggtgt gagactcacg
     2221 gacatggaat cggtgttccg cgaatccgat ttcgtcgtgg tgaactgccc gctcaacgag
     2281 gaaacacgca agctggtcgg cgccaggcag ttctctgtga tgaagccgac tgcgtttttc
     2341 atcaatacgg cgcgaggacc gattgtcgac gagaaggccc tgctcgacac gcttgccaac
     2401 cgccgcatcg ccggcgccgg ccttgacgtg ttcgagcagg aacccactcc ggcggacaat
     2461 ccgctgctga agctcgacag catcgtggtc accccgcacc acatctgcct taccgacgag
     2521 tgcatcaaca ccatcgccgc cagcgtgttc agcgcctgcc gcgatctcgc ccacgcccgc
     2581 gtgcccagga atgtggtgaa ccaggaagtt ctcggcaagg tgccgtattt ccacagatag
     2641 aggccgtgac tacgtgactg ccgtaacgac gtgactcctg ggaaacactg accaagcaac
     2701 tgccttactt gcgacagtca cgacagtcac gacagtcacg aagtcacggc atccttatga
     2761 gacccaaccc actcaagaaa agactgcttg atggtgccag cgcttacggc ctgttcgcgt
     2821 tcgaattctt tacgccggga ctggcacaga tcgctaagga ggccggtgcc gagtgcgttc
     2881 tattcgacat ggagcattcc ggggccggca tcgacgccat caagcagcag ctggcgtact
     2941 gtcgcggact cgacattgtt ccgttcgtgc gcgtgccgac gcatcagtat cactttgttg
     3001 cgcgggcatt ggacgcggga gcgctcggaa tcatggtgcc gatggtcgaa accgcgcggc
     3061 aggcgcgcga catcgtgtcg tggacccgct atcctccgcg cggccgccgg ggtgcggtgc
     3121 ttggcgccgc ccacgacgat tacagcggcg gcagcatgaa ggagaagttc gcgctggcaa
     3181 acgaacggtg cttcctgatg ctccaggtcg aaaccgaggt gggcgtggag aacgtcgaag
     3241 agatcgcggc ggttccggac gtggactgca tctgcattgg ttacgcagat ctctcgaact
     3301 tcctcggcgt gccgggagag ctgaagcatc caaagtatct cggtgcggtc aagcggattg
     3361 ccgcggcggc aaacaagcat ggaagggtcc ttgccactgc cgcaccggac gagatcttcg
     3421 caaaggaata tgccgcgctg ggcttcagga tgttcttctt cggcaccgat gcgcacctgc
     3481 tgcaatcggc actcgcccga cggattgccg caatgcggga aggcgggcgc tgacagcttg
     3541 ccggtggcct gccggaccgg caaactatgg gcctgcgggc gacaacagag ggaaggagaa
     3601 gcagctgtga ttgcggtacg aaaatcggat gcctgcatgg gggcggagat ctccggagtc
     3661 gatctgtcgc gtccgttgtc cgaccggatt tccggcgcag tgcgcgatgc gctgcaccgc
     3721 caccacgtgc tggcgttccg cggccagtcg ctggagcctg aaagcttcct ggaattcgcc
     3781 agccgtttcg gccggcccga gccgcatgtg ctcgaccagt ttcatcatcc cgagtacacc
     3841 gacatcctgg tactgtcgaa cgtgaagaag aatggcgagc ccataggatt ggccgacggc
     3901 ggcacgtact ggcacagcga ttattcctac ctgcagatcc ccgcgcgcgc tacgctgctg
     3961 tattcgatcc aggtgccgaa gatcggcggc gacacgctgt tcgcggacca ggagcaggcc
     4021 tacgaagagt tggccgaggc gatgaaaaag cgcatagacg gaatggtgac gctgaacgtc
     4081 tacggcaacc gcgacgacct cgatgtgagt tcgcgtactt cggcattcgc gccgaccgag
     4141 gagcagaagg agaagcgcgg tgcggccctg atccgccatc cgctggtgcg ccgccatccc
     4201 tacaccgggc gcaaggcgtt atatgcggta tcgggtacct cgttcgccat cgagggcatg
     4261 cccgacgacg agggcctctc tttgctgcgc gagctggccg cgcattcgac ccagcctaag
     4321 taccggtacc ggatgaagta cggtgtaggc gacatggtgg tgtgggacaa cgcatcggtg
     4381 ctgcactcgg cgaccctcac cgatccggac gatgcacgta cgctgttccg catcaccgtg
     4441 aaggaagcac aacggcctgt tgcctgaaag aaaaacccct tggcgcaaaa acgcgaagaa
     4501 aggcggaaat aagtatgaat tgccggaggg cgcagcgttc cgacatgagt gttccatgaa
     4561 cttgcccgtt gacacgagga tataccgtaa ggtcctccgt gtttctttgc gtctttgcgc
     4621 ctttgcgtcg aagtcgggat tcttgggtgt ttgttttacg gccgcacgtg ctgctgctct
     4681 gctgggcgtc gtgctcgtcg cggcggcggc atggtcggcc gacagcatgc tcgtcatcga
     4741 aaacaccgcc atcgaaaaga agctgcgcga gcagttcttc accgacaagg gccgtttctg
     4801 gctcaacaac gcggatgtgt gcaaccagtc ctggctggaa tcgccgaaag tcacggcggc
     4861 ggcaggcagg ctgcgcatcg gcgtgttgtt caacggccgc gtgggcgcgc aggtcggcgg
     4921 ccagtgcgtt ggcggctcgg acgccttcaa cgttacggct tccggcaagc cttttttccg
     4981 ggatggccgt ctgggcgtcg aagacatccg catcgactct ctgtccaagg agatgtaccg
     5041 gccgttgctg caacctgtat tcagttcgat ggtgcccaag gcgctggatg tgaacctcaa
     5101 ggaaacggtg gttgggctgc tggccgggcg caccgcgccg tacgaggtgc aaatcaatga
     5161 gatcgttgcc actgaggtca gcgcggatgc ggatcgcgtg aatgtcaggc ttcagttcga
     5221 tctgcgcgtg cgttgagaag gaaaatgacc gggccggttg ctcgacggaa aatcgcggca
     5281 cgtggtgcca aatgaatcaa gattcgctac ctgaaccacg gaggacacgg aggacacgga
     5341 gaattccagg agactcgatt ccagaactcc agaaaacccg gcgttcggtc aacgatggtc
     5401 tcagattcca atgtctttga gtctggccgc attcgttcac ctctaattga tctcatgctt
     5461 ttcctccgtg acccccgtgt cctccgtggt tcactctttt cgctcttcct ggcgttggcg
     5521 gcggcgcagg cgtcgccgta tcccgagccg gtccacggcg atttcgtgat ccgcgatttc
     5581 cgttttgcgg gcggcgaagt gctccccgaa ctgaagctgc actatttcac gctcggcaac
     5641 cccgcccggg ataccaatgg ccgcgtgacc aacgcggtgc tgctgctgca cggcactggc
     5701 ggcaaaggga cgaactttct gaccgacggt tttgccggcg aactcttcgg cgcgggccag
     5761 ccgctggatg cgcgccgttt ttacatcatc gttccggaca acctgggaca cggaaaatcc
     5821 accaaaccca gcgacgggtt gcgcgcgaag tttccgcgct acgggtatgc ggacatggtc
     5881 gcggcgcagc gtcggctggt gacggatgga ttgggggtcc ggcatctgcg gctgatcatc
     5941 ggcacctcga tgggcggaat gcacgcctgg atgtgggcag aaagcgaacc ggatttcatg
     6001 gacgccgtcc tgccgatggt ctgtctgccc gatcagattt ccgggcgcaa ccgcatggag
     6061 cggcggctca tggtcgatgc catccgcaac gatccggagt ggaacggcgg cgactacgcg
     6121 aagcagccgc aggggctgat aaccgcattc aaaatggtga tcatcggcgc gggcagcgta
     6181 aaacagtatt accttgaggc gccgacccag gaggcgaccg accgcctgtt ggaccggcaa
     6241 ctgcagcaac gacttcaggg cacggacgcc aacgatttcc tttacgcatg ggaggcatcg
     6301 cgcgactacg atcccggccc ggggctggag cgtatccgcg cggcggtgac cgcggtcaat
     6361 ttcgaggacg acgagcgcaa cccgcccgaa ctcggcatca tggagcgcgc catcaagcgc
     6421 gtcgcgcgcg gaaaatacgt gctcgtaccc gcaagtgagc gcactcgcgg ccatgcgagt
     6481 ttctacgacg tcggtttgtg gaaggaggac ctggagaagt tgctggagcg gtctgcgcgc
     6541 tgagctaggg cctaaataca ctacacctca acgcaaagac gcaaagagcg taaaggaacg
     6601 caaagaaaga aaacgaaaat ctcaatgcag cggcaggacg atgggtaatc cgagctttgt
     6661 ggtttctacc tgcgctcaag ttgtgcggct agatgctgaa ttcccaatgc actgcacttt
     6721 tttcagcgtt cgtttcattc ttcccgtttt tctttgcgtc ctttcttgct tttactttgc
     6781 gatctttgcg tcaacactct ttatgccggc ccttggccgg ctttcgacta gtcaaagcgg
     6841 cttgttagga tcgatgatgg tgcgccacat gtgcgcagtg ggactcgaat cgttgcccag
     6901 gccttccctg ggctggcgaa agtgccggcc gatgatgtcg gcaaaaggct cgagcggcca
     6961 cggcgcattc cggtcgaagc tgtagtcgtc gaacaccgtg atctgcctcg cgaccatgta
     7021 ccaggggtgg ttgcgagcgt agcgatacgc cgcttcgatg tgttccggcg gccggtagtt
     7081 ctctccgaac atcgcgacgt acatcgccgg atactcgtaa ccggccgact cgtccgggta
     7141 gtaacgcagc gcctggtggt agcggatcgc ccaggcgact ttctcggaga cataaggctc
     7201 caccaactgc gcaccccacc aaccgtggtc gggccgcatg accaccatcc ccacgtcgtg
     7261 cagcaggcag gccagcacgg tttcctcgtc catgcccgct ttcagcgcca gcgttgcgca
     7321 ttgcacgcaa tgcgatccgg ccgcgaacgg cattccgaat ttgggcttgt ccatccgcaa
     7381 catgaatata tccgccagcg cgggccgggc cggcatgtcc gccaggatct cggtcgcttt
     7441 ggacgcagcg gtgccggtgc cgatctcttt cagggtttgc aggcggtcat ttttcatggt
     7501 gccggagacc tgaggtctgc aaccgtcctc cctattccgg gtcggaattg tcgtccgacc
     7561 accggcgttt cggcccgccg tgggaatctt cctccgggat cgaaggcatg tcgtcgtcga
     7621 ccgcgtcgtc gaacttctcc atctcccgga tcagcgattg cagccgcttt tccgcggtag
     7681 tttcccgatc cggttgattg acgatggtcc tgacgacgga aaccgcgcct ttgaagtctc
     7741 gctcggtctt gatcggacgg gcatgtccgt ttagtttcat tgggctgatt cgcgacatgt
     7801 gtgcctcccc tgaaaatcca gccttccgat gcctcgccgt cagaaacacg ccttgacgac
     7861 cccgccgtcc actcgcaatg cggcgccgtt ggtggcggag gaaagcgggc tgcaaacgta
     7921 ggcaaccagg ccggcgactt cctcgggagt agcataacgc tttagcaggg aagtggggcg
     7981 atggac
//
LOCUS       DASQOP010000146        14417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_659470, whole genome shotgun sequence.
ACCESSION   DASQOP010000146 DASQOP010000000
VERSION     DASQOP010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14417
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659470"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VE030_06000"
     CDS             <1..708
                     /locus_tag="VE030_06000"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF003743.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HZF23002.1"
                     /translation="FISDLHLSPDRSAATEALMRFLRDSAPAAEALYVLGDLFEYWIG
                     DDGLGHPFARSIADAFRKLSDGGVPVSFMHGNRDFLIGNRFARESGMQLLSDPTLISL
                     YGPRTLLMHGDTLCSDDVEYQKFRAMVRNPAWQQAFLARPLAERARMAQDVRGKSEQA
                     KQVKDMVIMDVAPATVEDVLRSHAYPRLIHGHTHRPARHEHVVDGRLCERWVLADWYE
                     QGSYLLCDAAGCRVVPI"
     gene            complement(718..1899)
                     /locus_tag="VE030_06005"
     CDS             complement(718..1899)
                     /locus_tag="VE030_06005"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HZF23003.1"
                     /translation="MRWDELELETLAVRAGTHRSQFNEHSEALYLTSSFVFKDAAEAA
                     ARFSGEDPGMVYSRYTNPTVSALQERLAVLEGAECCIATSSGMSAILGCVMALMKAGT
                     HIVSSRSIFGATVQLFGNMMQRFGVDTTFVPATDVAAWEKAIRPNTRLLFLETPSNPL
                     TEVSDVAALADVAHRHGALLVVDNCFCSPALQRPLELGADIVMHSATKYLDGQGRVLG
                     GAVLGRRELILDGMTAFLRSCGPTLSPFNAWVILKGLETLGLRMQAHSKGALELAQWL
                     EQHPKVERVYYPGLASHPQHALAMRQQKSGGGIVSFDVKGGREAAWKVVDSTQLISIT
                     ANLGDTKTTITHPASTTHGRVAPEARKAAGIGEGLLRIAVGLEAVSDVKVDLARGLSK
                     L"
     gene            complement(2114..3634)
                     /gene="purF"
                     /locus_tag="VE030_06010"
     CDS             complement(2114..3634)
                     /gene="purF"
                     /locus_tag="VE030_06010"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HZF23004.1"
                     /translation="MCGLLGVVAKSPVNQLLYDGLQVLQHRGQDAAGIVTAEGSKFQM
                     HKGGGLVRDVFRTRDMRHLVGNIGIAHCRYPTAGSASSFAEAQPLYVNSPFGIVLGHN
                     GNLTNAEQLKRDLFRQDLRHVNTNSDSEVLLNVLAHELQEAANGYQLDPPTIFQAVSG
                     VHRRCRGAYAVVAMIAGYGLLAFRDPYGIRPLVVGMTKTEKGAEYLVASESVALDTLG
                     FHRVRDVAPGEAIFIDYEGNFYSRQCAANPQHNPCIFEFVYLARPDSVIDGISVYETR
                     MRMGASLAEKIKREYRDLDIDVVIPIPDSSRPSAMELAENLGLPYREGFIKNRYIGRT
                     FIMPGQEVRKKSVRQKLNAIGMEFKGRNVMLVDDSIVRGTTSREIVQMARDAGARKVY
                     FASAAPPVRYPNVYGIDMPTSRELIATGRTDDEIAREIGADRLIYQDLDALIDDVRSV
                     NPKIEHFETSCFNGVYVTGDITPEYLADVEAQRNDDELSRREANVTQLDLNLATAE"
     gene            complement(3709..4200)
                     /locus_tag="VE030_06015"
     CDS             complement(3709..4200)
                     /locus_tag="VE030_06015"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HZF23005.1"
                     /translation="MTVFDLIVIAIVAASVGISIWRGMVREVMALASWIGAFWIAKEL
                     AGFTATWLPDSVSNPGLRLMFGFIAVMLASLLVFSLIGMFIVHLVKVAGLTASDRMLG
                     AAFGLLRGVAIAVTVVLLGGMTSAPREPYWRDALLSAPLETAALWVKPWLPRDVARRV
                     SFE"
     gene            complement(4290..4892)
                     /locus_tag="VE030_06020"
     CDS             complement(4290..4892)
                     /locus_tag="VE030_06020"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HZF23006.1"
                     /translation="MAKAISDEELQLKKRARRRLVGAIALVLLVVVFLPMFLDSEPKP
                     LNQDVAIIIPPLPGPDSSSRPATAAPAGLAPSGAVVPPAKTETAPLRPAPVPVALPKA
                     ETPKPEPSPAPVQLAKTSEDFVVQLGAFSNAANARALQRKLQENKFKAYTEVVKSSGT
                     ERTRVRVGPYDSREAAEKARQRIRTLKLAIGDATVVRQGD"
     gene            complement(4916..6193)
                     /gene="folC"
                     /locus_tag="VE030_06025"
     CDS             complement(4916..6193)
                     /gene="folC"
                     /locus_tag="VE030_06025"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HZF23007.1"
                     /translation="MSIDRATAPQSLSDWLSYLERIHPWAVDMGLERVARVRDRLGLV
                     STFPIIAVAGTNGKGSVCAMLESILSSAGYKIGVYTSPHLLRYNERVRVQGQEAADEE
                     LVRAFTRIEQVRGEVSLTYFEFGTLAAMDVFIRERVDLAVLEVGLGGRLDAVNAFDSD
                     CAVVTSVDFDHMEFLGNSRDSIGAEKAGIFRAGRPAICGEGDAPAGLLGRAAEIGVKL
                     HLIGRDYGFNADPNQWQFWSAQGKRSALPYPALRGDYQLGNAATALCALEQLKDRLPV
                     DMGAIRRGLLDVQLPGRFQVLPGRPMVILDVGHNPHAARALASSLRRLPGGGRTIAVF
                     AMLKDKDIEGVAAAMRDDVDEWLVAGLSGPRGADAERIRQALAAARVGVPVAKFDTPL
                     GAYQHAIRSGGQNDKILVFGSFYTVGAVLAARG"
     gene            complement(6233..7096)
                     /gene="accD"
                     /locus_tag="VE030_06030"
     CDS             complement(6233..7096)
                     /gene="accD"
                     /locus_tag="VE030_06030"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HZF23008.1"
                     /translation="MSWLQKLLPPKIKRSAGAVKKAVPEGLWSKCEVCETVLYRADLD
                     KNLNVCPKCGHHNRLTARERIDLTLDPEGQFEIAAEVAPIDFLKFVDSKRYTERLEQA
                     KAETEEDDALVVVQGSIKNVPLVAAAFEFRFLGGSMGSVVGERFVRGVQSCCEQNLPF
                     VCFSASGGARMQEGLMSLMQMAKTTAALTQLSRARLPFISVLTDPTMGGVSASFAMIG
                     DVVIAEPGALIGFAGPRVIEQTVRQTLPEGFQRAEFLLEHGAVDMIVDRRQMRDKLAG
                     LITLLMRAPAA"
     gene            complement(7167..7967)
                     /gene="trpA"
                     /locus_tag="VE030_06035"
     CDS             complement(7167..7967)
                     /gene="trpA"
                     /locus_tag="VE030_06035"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012217295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HZF23009.1"
                     /translation="MSRIPDTFKKLQSQGKKALIPFVTAGDPNPAMTVPIMHALVGAG
                     ADIIELGVPFSDPMADGPIIQRSSERALKHGVGLKHVLGMVREFRTTNRMTPVVLMGY
                     ANPIEAMGCAAFAETGQAAGVDGVLVVDYPPEESRQLTDLLKARGIDSIFLLAPTSED
                     SRMEQVGRMATGYIYYVSLKGVTGAAHLDLQQVTAKLPQIRSHIRLPIGVGFGIRDAQ
                     TAKAIASISDAVVIGSRLVQEIESSGPDQVLGNLSALLGGIRRAMDGT"
     gene            complement(8060..9271)
                     /gene="trpB"
                     /locus_tag="VE030_06040"
     CDS             complement(8060..9271)
                     /gene="trpB"
                     /locus_tag="VE030_06040"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017329990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HZF23010.1"
                     /translation="MYETLKAYNLPDAKGHFGPYGGVFVAETLMHALDELRTQYERMR
                     NDAGFVAEFESELKHYVGRPSPVYHARRLTEQAGGAQIWLKREDLNHTGAHKINNTIG
                     QALLAKHMGKKRVIAETGAGQHGVAAATVAARYGMECVVYMGSEDVKRQSMNVFRMKL
                     LGATVVPVESGSKTLKDALNEAMRDWVTNVESTFYIIGTVAGPHPYPMMVRDFNAVVG
                     RELRTQMPEAIGRQPDAILACVGGGSNAMGAFYPYIEDKSVRLIGVEAGGLGLASGKH
                     AATLCAGKPGVLHGNRTYLIQDENGQIIETHSISAGLDYPGVGPEHAWLKDSGRAEYV
                     SVTDDEALNAFHTLTRMEGIMPALESAHAVAYALKLAPTLPKGQVLLVNLSGRGDKDM
                     NTVAERSGLKL"
     gene            complement(9264..9887)
                     /locus_tag="VE030_06045"
     CDS             complement(9264..9887)
                     /locus_tag="VE030_06045"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HZF23011.1"
                     /translation="MATAVKICGITRIEDALAAAHAGAHAIGLIFAAESPRRVSTGLA
                     RKVVEALPAFVTPVALFVDPQPAEVERVLGEVGPQLLQFHGDETPDFCVRFGVPYIKA
                     ARVGPGVDLLQYAGRHRAAKGLLLDAFVEGSRGGTGSTFDWSLIPAELPLPLILAGGL
                     DPDNVGDAVRRVRPWAVDVSSGVEAATGIKDAAKIAAFIRGVRHADV"
     gene            complement(9910..10710)
                     /gene="truA"
                     /locus_tag="VE030_06050"
     CDS             complement(9910..10710)
                     /gene="truA"
                     /locus_tag="VE030_06050"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HZF23012.1"
                     /translation="MRIALGLEYDGAAFCGWQTQPDGCAVQDALERALSGVAGKNIET
                     TCAGRTDAGVHALAQVVHFDFDVERSPSAWIRGGNALLPDAIAVNWARAVSPDFHARF
                     SATGRRYVYWLLNRAQRPGLLRGRVGWFYRHLDEQAMQQAAQHLIGSHDFSAFRAAEC
                     QAKTPVKTLRQLEICRRSDLIRFEFAADAFLQHMVRNIVGALVYVGSGRQPAGWIGEV
                     LESRDRNRAAPTFAASGLYLAAVEYDASWALPSRPDIASIEELLIAAE"
     gene            complement(10823..13555)
                     /locus_tag="VE030_06055"
     CDS             complement(10823..13555)
                     /locus_tag="VE030_06055"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HZF23013.1"
                     /translation="MGKLNVTSALGEPLDAEIELFVEKKEISSLAARLATPEAFGRAG
                     LLYSNALDKAKVSIEKRANGEPYLRLTTTQPVNEPFVDLLVELSWSSGRISREFTALL
                     DPPSLIAERARQKAAEAQVRASPPKPEPQPEPIPEPPAQAPAAAAPAPEAAPAVESPA
                     AAQAPAKTEAPAVTEPAPSAPVETIGGPMATFLTESNINSTSRSSAATSAATGDSYGP
                     VTRGDTLAKIALATKPAGVSVEQMLVLLLRGNPDAFTGKNMNRLKTGKIIKLPPPDQY
                     GSVAQADARKEVLVQARDWHAYREQLAAKAGEAKPAAEPPQQAAAGKITAKVEDQAAA
                     ANQQPKEVLKLSQGEPAAAAAAPAGDAKAGQGQARVRALEEELVARDKAVKDANERVS
                     KLEKTIKDLQALLEIKNKGMADLQKPAAPPPAAATTPAQPAPAQPAPKPAAKPETHAT
                     VPVAPASAPEAKPAPAQAPVAPATPQPKIETAPPSQPAPPPKPAPKSAPPPAPEPSLL
                     DRVLEQPAYLGAGLGVLVLIGALAVRAVKRRRESKAEAEEAGPAPVVGAAAPSSPAFN
                     STDTDLSAASARAASDIAEEVDPVAEAEIFLAYGRDAQAEDLLKEALAASPTRYEIHL
                     KLLQIYANRKDTNAFEQLARELQKGTGGSGYLWDQAVTLGYQIDPDNPRYAAGRPSGG
                     ETAAVAAVAAGGAADNVDFNVGFEDPNATTATDIDLGDDGRKLDRTQVIDPSTLESHK
                     DSTILEPGAAPVMNFSVELPPAGTTAEEAEASKKQEGNTIDFDFDLNALAAPSASVPA
                     AEEPKPAGGGLDFDMSGISLDASGAPKTDSGAVQEIDLSGINLDLGTGSSPQVSAAGK
                     DDRWYDVQTKFDLAKAYQEMGDKDGAREILKEVLQEGDAEQKAAAQTVLANLDS"
     gene            complement(13788..>14417)
                     /locus_tag="VE030_06060"
     CDS             complement(13788..>14417)
                     /locus_tag="VE030_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012338485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HZF23014.1"
                     /translation="QAASGAGAQNMRELVSQMGYIHGAAKSLLEDPASSILEIDRKVS
                     DALRSRALPSEHFGLPLAGSLIPWIDKDLGNGQSREEWKAQAEGNKILGREANPVPID
                     GVCVRIGAMRCHSQALTIKLTKELPLEEIEGLLASANQWVKVVPNQREASMRELTPAA
                     VTGTLMVPIGRLRKLPMGGEYLAAFTVGDQLLWGAAEPLRRMLRILLEQ"
BASE COUNT         2465 a         4712 c         4565 g         2675 t
ORIGIN      
        1 ttcatctccg atctgcatct gtctccggac cggtcggcgg cgaccgaggc gctgatgcgc
       61 tttctgcgcg actccgcgcc tgcggccgag gccttgtacg tgctcggcga cctgttcgag
      121 tattggatcg gcgacgacgg gctggggcat ccgtttgccc gcagcatcgc cgacgcattc
      181 cgcaagctgt ctgatggcgg cgtgcccgta tccttcatgc acggcaatcg cgacttcctg
      241 atcggcaacc ggttcgcgcg cgaaagtggc atgcaactgt tgtccgaccc gaccttgatc
      301 agcctgtacg gtccgcgcac cctgctgatg cacggagaca cgctgtgcag cgacgacgtg
      361 gagtatcaga aattccgcgc catggtgcgc aaccccgcct ggcagcaggc cttcctcgcc
      421 aggccgctgg ccgaacgcgc gcgcatggcg caggacgttc ggggcaagag cgaacaggcc
      481 aagcaggtca aggacatggt gatcatggac gtggcgccgg caacggtcga agacgtgttg
      541 aggagccacg cctacccacg gctgatccac ggccacaccc acaggccggc ccggcacgag
      601 catgtcgtcg atggccgctt gtgcgaacgc tgggtgctgg ccgactggta cgaacagggg
      661 tcgtacctgc tttgcgatgc ggccggttgc cgcgtcgtgc cgatctagta accggcgcta
      721 cagcttcgac agaccgcgcg ccaggtccac tttgacgtcc gaaacggctt ccaggcccac
      781 cgcgatgcgc agcaagccct cgccgattcc cgccgccttg cgcgcctcag gcgcaacgcg
      841 cccgtgcgtg gtgctggccg gatgggtgat cgtggtcttg gtatcgccca agttcgcggt
      901 aatggaaatc aactgcgtcg aatccaccac cttccacgcg gcttcacgtc cgcccttcac
      961 atcgaaggag acgatgccgc cgccgctctt ctgctggcgc atcgccagtg catgctgcgg
     1021 atgcgaagcc agtcccggat aatagacgcg ttccactttc gggtgctgct ccagccactg
     1081 cgccagctcc agcgccccct tggagtgcgc ctgcatgcgc aggcccagcg tctccaggcc
     1141 tttcaggatc acccaggcat tgaacggcga cagcgtcgga ccgcaactgc gcaggaaagc
     1201 agtcatgccg tcgagaatca gctcgcgcct gcccagtact gcgccgccga gaacgcggcc
     1261 ctggccgtcc agatacttgg ttgcagaatg catgacgatg tcggccccga gttcgagcgg
     1321 ccgctgcagc gccgggctgc agaagcagtt atccaccacc agcagtgcgc catgccggtg
     1381 cgccacatcg gccaatgccg ccacgtcgga aacctctgtc aagggattgg acggcgtttc
     1441 caggaacagc aggcgcgtgt tcggccgtat cgccttttcc catgcggcca cgtcggtcgc
     1501 gggcacgaac gtggtgtcca cgccgaagcg ctgcatcatg ttgccgaaca actgcacggt
     1561 cgcgccgaaa atgctgcgcg acgagacgat atgggtgccg gccttcatca gtgccatcac
     1621 gcaaccgagg atcgccgaca tgccggatga cgtggcgatg cagcactccg cgccctcgag
     1681 tacggcgaga cgttcctgca gcgcgcttac cgtggggttg gtgtagcgcg agtacaccat
     1741 ccccgggtcc tcgccggaaa agcgcgccgc cgcctcggcc gcgtccttga agacgaagct
     1801 tgacgtcagg tacagcgctt ccgaatgctc gttgaactgg ctgcggtgtg tgcccgcgcg
     1861 cacagccagc gtttccagtt ccagttcgtc ccatctcatc gcattgtccc gatcgcgttg
     1921 cacgccgcgt tcgcggcagc cggaaaaaaa caaaacccgc ttgagctttc gctaagcggg
     1981 tccggaatcc gacacacgct ttagcagtat ttgttacgcg cccgcaagct gttcgtcaaa
     2041 tcggcgcgaa atcacacgct actcctggcg cgcgggcctg tcaatcgccg gcaccggagc
     2101 agatacgaga ggtctattcg gcggtcgcca ggttcaggtc cagttgggtg acgttggcct
     2161 cgcggcgcga caactcgtca tcgttgcgct gcgcctcgac gtcggcgagg tactccggcg
     2221 tgatgtcgcc ggtgacatag acgccgttga agcacgaggt ttcgaaatgc tcgatcttcg
     2281 gattgaccga gcgcacgtcg tcgatcaacg catccagatc ctggtaaatc aagcggtccg
     2341 cgccgatctc gcgggcgatc tcgtcgtcgg tacggccggt agcgatgagc tcgcgcgagg
     2401 tcggcatgtc gatcccatag acgtttggat agcgcaccgg cggagccgcg gacgcgaaat
     2461 agaccttgcg cgcacccgcg tcgcgcgcca tctgcacgat ctcccggctg gtggtgccgc
     2521 gaactatgga atcgtccacc agcatcacat tcctgccctt gaactccatg ccgatggcgt
     2581 tcagtttctg gcgcaccgac ttcttgcgca cttcctggcc cggcatgatg aaagtgcggc
     2641 cgatgtagcg gttcttgatg aagccctcgc ggtaaggcag cccgaggttc tcggcaagct
     2701 ccatcgcaga ggggcggctg gagtccggga tcgggatcac cacgtcgatg tccaggtcgc
     2761 gatactcgcg cttgatcttc tcggcgaggc tcgcgcccat gcgcatgcgc gtctcgtaca
     2821 ccgaaatgcc gtcgatcacc gagtcggggc gggccaggta cacgaactcg aaaatgcagg
     2881 gattgtgctg gggattcgcg gcgcactgac ggctatagaa gttgccctcg taatcgatga
     2941 atatcgcctc gccgggcgcc acgtcgcgca cccgatggaa acccagggta tcgagcgcca
     3001 cgctctccga ggccaccagg tattccgcgc ctttttctgt tttcgtcatg cccaccacca
     3061 gcgggcggat tccgtaagga tcacggaacg ccagcaaccc atagcccgcg atcatcgcta
     3121 ccaccgcgta agcgccgcga cagcggcggt gcacgccgga caccgcctgg aagatggtcg
     3181 gtggatcgag ctggtagccg ttggcagcct cctgcagttc gtgcgcgagc acgttgagca
     3241 gcacttccga gtcggaattg gtgttgacgt ggcgcaggtc ctggcggaac agatcgcgct
     3301 tgagctgctc ggcgttggtg agattgccgt tgtggccgag cacgatgccg aacggagaat
     3361 tcacgtacag cggctgggct tcggcaaaag aagaggcgga tccggcggtg ggataacggc
     3421 aatgggcgat gccgatattg ccgactaggt ggcgcatgtc gcgggtgcgg aacacatccc
     3481 gtaccaaccc gccccccttg tgcatctgga atttactgcc ttcggcggtg acgattccgg
     3541 cggcgtcctg accccggtgc tgcagcacct gcagcccatc atagagcagc tggttaaccg
     3601 gcgatttggc tacaacgccc agaagtccac acatcgctca ggcctccaca cccactggcg
     3661 ccggcccggc agccggcgca atgcaggtgc aagtatagtc taatccgctc actcgaagct
     3721 gaccctgcgg gccacgtcgc gcggcagcca gggctttacc cacagcgccg cggtctccag
     3781 cggcgccgac aacagcgcat cgcgccagta aggctcgcgc ggcgccgaag tcatcccacc
     3841 gagcagcacc acggtcaccg cgatcgcgac accgcgcaac agcccgaacg cggcgcccag
     3901 catccggtcc gaggcggtaa ggcccgcgac tttcaccagg tgcacgatga acatgccgat
     3961 aaggctgaac accagcaggc tggcgagcat caccgcgatg aaaccgaaca tcagccgcaa
     4021 cccgggattg gacaccgaat ccggcagcca ggtcgcggta aatccggcca gttccttggc
     4081 aatccagaat gccccgatcc acgaggccag ggccatcact tcacgcacca ttccgcgcca
     4141 gatgctgatc cccaccgatg ccgccacgat cgcgattacg atgaggtcga aaaccgtcat
     4201 cgggagcggt tcgctccccg gtgaaccggg tagatggtca ttgcgggacg gggattcaag
     4261 tgccttcgca tagtccccgg tcttcggact cagtcgccct gccgcaccac ggtggcgtcg
     4321 ccaatcgcca gcttcagggt gcgtatgcgc tggcgcgcct tttcggcggc ttcgcgtgag
     4381 tcgtagggac ccacgcgcac gcgcgttctt tcggtgccgg aacttttgac gacctcggtg
     4441 taggccttga acttgttttc ctgcagcttg cgctgcagcg ccctggcatt ggcggcattg
     4501 gaaaaggcgc cgagttgcac tacgaagtcc tcgctggtct tggccagctg caccggtgcc
     4561 ggactaggtt cgggcttggg cgtctcggcc ttcggcagcg ctaccggcac gggcgcaggc
     4621 cggagcggcg cggtctcggt cttggcgggg ggaacgacgg cgcccgaggg ggccaggccg
     4681 gccggcgcag cggttgcggg ccgcgaggac gaatcggggc cgggtagcgg aggaatgatg
     4741 atggcgacgt cctggttcag cggcttcggc tcgctgtcga gaaacatcgg cagaaacacg
     4801 acgaccagga gcaccagtgc tattgccccg accagccgcc gccgtgcacg tttcttcagt
     4861 tgcagttctt cgtcgctaat cgctttcgcc atatcgttgt gaatcggcgc cggattcacc
     4921 cgcgcgctgc cagcaccgca cccaccgtgt aaaaggagcc aaacaccaaa attttatcat
     4981 tttggccgcc ggatctgatc gcatgttgat acgcgccgag cggggtgtcg aacttcgcca
     5041 ccggcacgcc gacccgcgcc gctgccagcg cctgccggat tcgttcggcg tcggcaccgc
     5101 gaggcccgga aaggccggcg acaagccact cgtccacgtc gtcccgcatt gctgccgcca
     5161 cgccttcgat atccttgtcc ttgagcatcg cgaacaccgc aatcgtcctg ccgccgccgg
     5221 gcagccgccg cagactggat gccagtgcac gcgcagcgtg cgggttatgg ccgacatcga
     5281 ggatcaccat gggccggccg ggcaatacct gaaagcgccc cggcaactgc acgtcgagca
     5341 gtccgcgccg gattgcaccc atgtccaccg gcaaacggtc cttcagttgc tccaacgcgc
     5401 acaatgcggt agcagcgttg ccgagctgat aatcgccacg cagcgcgggg tacggcagcg
     5461 ccgagcgctt gccctgcgca ctccagaact gccattgatt cggatcggca ttgaaaccgt
     5521 agtcgcggcc gatcaaatgc agcttcacgc cgatttccgc cgcgcgccca agcagtccgg
     5581 cgggcgcatc cccctcgccg cagattgccg gccggccggc acggaaaatc ccggccttct
     5641 cggctccgat gctgtcgcgg ctgttgccga ggaattccat atggtcgaaa tccacgctgg
     5701 tcaccaccgc acaatcggaa tcgaacgcgt tcaccgcgtc cagcctgccg ccgaggccga
     5761 cttcgaggac ggctagatcc acccgctcgc ggatgaaaac gtccatcgcc gccagcgtgc
     5821 cgaactcgaa gtaagtgagc gagacctcgc cgcgcacctg ctcgatgcgc gtaaacgcgc
     5881 gtaccagttc ctcgtccgcc gcttcctggc cctgtacgcg cacccgctcg ttgtaacgca
     5941 gcaggtgcgg cgaggtgtag acgccgatct tgtagccggc gctggaaaga atgctctcca
     6001 gcatcgcgca caccgagcct ttgccgttgg tgccggccac tgcaatgatc gggaaagtgg
     6061 agaccaggcc gagacgatcg cgcacgcggg caacgcgctc cagtcccata tcgacagccc
     6121 aggggtggat gcgctcgagg taggaaagcc agtcggaaag cgactgcggc gctgtggcgc
     6181 gatcgatgga cacgtcgagt gccggcctgc gggggccggt aagggaatag agctaagccg
     6241 ctggggcgcg catcagcagc gtgatcaggc cggcgagctt gtcgcgcatc tgccggcgat
     6301 cgacgatcat gtcgacggcg ccatgctcga gcagaaactc ggcgcgctga aagccctccg
     6361 gcaaagtctg gcgcacggtc tgttcgatca cccgcgggcc ggcgaaaccg atcagcgctc
     6421 caggctcggc gatcaccacg tcgccgatca tcgcgaagct tgccgacacg ccacccatgg
     6481 ttgggtcggt cagcaccgag atgaacggca atctcgccct ggaaagctgg gtgagcgcgg
     6541 cggtggtctt ggccatctgc atcagcgaca tcagaccttc ctgcatgcgc gcaccaccgc
     6601 tcgcggaaaa gcagacgaag ggaagattct gttcgcaaca actctgtacg ccgcgcacga
     6661 accgctcgcc caccacggaa cccatcgagc cgcccaggaa acggaactcg aaggccgccg
     6721 ccaccagcgg cacgttcttg atcgagccct gtaccaccac taaggcgtca tcctcctcgg
     6781 tttccgcctt ggcctgctcg aggcgctcgg tatagcgctt gctgtccacg aacttgagga
     6841 agtcgatggg cgcgacctcc gcggcgattt cgaactggcc ttccgggtcg agcgtgaggt
     6901 cgatgcgctc gcgggcggtg agccggttgt ggtggccgca cttgggacag acgttgaggt
     6961 tcttgtccag atcggcgcga tagagcaccg tctcgcagac ctcgcacttg ctccacagcc
     7021 cctccggcac ggccttcttg acggcgccgg cgctgcgctt gatcttcggc ggaagcagct
     7081 tctgcaacca gctcatccac gtccccccgt aactcgagcg acccgcaagc gcgggcgcga
     7141 ttcaattgcg cgtttcgaaa acagactcac gtaccgtcca tggcgcgacg gatgccgcca
     7201 agcaacgccg agagattgcc gagcacctgg tcgggaccgg aggattcgat ctcctgcacc
     7261 aggcggctgc cgatcaccac cgcgtcggaa atcgaggcaa tcgccttggc ggtctgcgcg
     7321 tcgcggatgc cgaagcccac tccgatggga agccggatgt gcgagcggat ctgcggaagc
     7381 ttggccgtga cctgttgcag atcgaggtgg gcagcaccgg tcacgccctt gagcgacacg
     7441 tagtatatgt atcccgtcgc catgcgcccg acctgctcca tgcggctgtc ttccgatgtc
     7501 ggcgccagca ggaaaatgga atcgatcccg cgcgccttga gcagatcggt cagctgcctg
     7561 ctttcctcgg gtggatagtc gaccaccagc accccgtcca cgcccgccgc ctgtccggtt
     7621 tctgcaaacg cggcacaacc catcgcctcg atcggattgg catagcccat cagcacgacc
     7681 ggagtcatcc gattggtggt gcggaattcg cgcaccatgc cgagcacatg tttcagtccc
     7741 acgccgtgct tgagcgcgcg ttcgctggag cgctggatga tcggcccgtc ggccatcgga
     7801 tcggaaaagg gcacgcccag ttcgatgatg tccgcgccgg cgccaaccag cgcgtgcatg
     7861 atcggcacgg tcatggccgg attggggtcc ccggcggtga cgaagggaat cagcgccttc
     7921 ttcccctgtg actggagttt cttgaacgta tccggaatgc gggacatgtt taggcaaggt
     7981 ggaaggatga aggatgaagg cggaagtgaa aaacctgggt tacgttcgtc tttccgtctt
     8041 ccggcttcat ctttggttgt tacagcttga gaccggaacg ctcggcgacg gtattcatgt
     8101 ccttgtcgcc gcggccggaa agattcacca acagcacctg ccccttgggc agcgtgggcg
     8161 ccagcttcag tgcgtacgca accgcgtgcg cggactccag cgccggcatg atgccttcca
     8221 tccgggtcag cgtgtgaaag gcattgagcg cctcgtcatc ggtgaccgag acgtactcgg
     8281 cgcgcccgct gtccttgagc cacgcgtgct ccgggcccac gcccggatag tcgagaccgg
     8341 cggaaataga atgggtttcg ataatctgcc cgttctcgtc ctggatcaga taggtgcgat
     8401 tgccgtgcag cactccgggt ttgccggcgc acagcgttgc cgcatgctta ccggatgcga
     8461 ggcccagtcc gccggcttcg acgccgatca gccgcaccga cttgtcctcg atgtagggat
     8521 agaacgcgcc catggcgttc gagccgccgc cgacgcaggc gaggatcgcg tccggctggc
     8581 ggccgatcgc ttccggcatc tgggtgcgca gctcgcgccc gacgactgca ttgaagtcgc
     8641 gcaccatcat cgggtaggga tgcgggccgg cgaccgtgcc gatgatgtaa aaagtgctct
     8701 cgacgttggt gacccagtcc cgcatcgctt cgttgagcgc gtccttcagc gttttcgagc
     8761 cactttccac cggcaccacc gtggcaccca gcagcttcat ccggaacacg ttcatcgact
     8821 ggcgcttgac gtcttccgac cccatgtaca ccacacactc catgccgtag cgggccgcga
     8881 cggtagcggc ggccacgccg tgctgaccag cgccggtttc ggcaatcacc cgcttcttgc
     8941 ccatgtgctt ggcgagcagc gcctggccga tggtgttgtt gatcttgtgc gcgccggtgt
     9001 gattcaggtc ttcgcgcttg agccagatct gcgcgccgcc ggcctgttct gtcaggcgtc
     9061 tggcgtgata caccgggctg ggacggccga cgtagtgctt gagttcggac tcgaattcgg
     9121 cgacgaatcc ggcgtcgttg cgcatgcgct catactgcgt gcgcaactcg tcgagcgcgt
     9181 gcatcagcgt ctcggcgacg aacacgccgc cgtacggtcc gaagtggccc ttggcgtcgg
     9241 gaaggttgta agccttgagg gtctcataca tctgcatgtc tcactcctcg gatgaaggca
     9301 gcgatcttgg ccgcgtcctt tattcctgtt gcggcctcga caccgctgct cacatcgacc
     9361 gcccacgggc gcacgcggcg cacggcgtcg cccacgttgt cgggatcgag cccgccggca
     9421 aggatcagcg gcaggggaag ctcggccggg ataagcgacc agtcgaatgt ggatccggtg
     9481 ccgccacggc tgccttccac gaacgcatcg agcagaaggc ctttggcggc gcgatgacgg
     9541 ccggcgtatt gtagcaaatc cactcccggc ccgaccctcg cggccttgat gtagggcacg
     9601 ccgaatcgca cgcaaaaatc cggcgtctcg tcgccgtgaa actgcagcag ctgcggacca
     9661 acttcgccga gcacgcgttc gacttctgca ggctgcggat caacgaacaa cgccaccggc
     9721 gtaacgaatg ccggcagcgc ctcgacgacc ttacgcgcga gcccggtcga caccctccgc
     9781 ggactttcgg cggcgaagat caggccgatc gcatgcgcgc cggcgtgcgc ggcggcaagc
     9841 gcgtcttcga tacgggtaat gccgcagatc ttgaccgccg tggccatccc gactcaggac
     9901 tcctgaaaat tattctgccg cgatcagtaa ttcttcgata ctggcaatgt ccggacgcga
     9961 tggcagcgcc caggacgcat cgtattctac cgctgcaagg tagagtcccg aggccgcaaa
    10021 tgtgggtgcg gcacgattgc gatcgcgact ttccagcact tcgccgatcc agccggcggg
    10081 ctgtctgccg ctgccgacat agaccagcgc gccgacgatg ttgcgcacca tatgctgcag
    10141 gaaagcgtcg gcggcaaact cgaagcgaat caggtcgctg cggcggcaga tctcgagttg
    10201 gcgcagggtc ttgaccggcg tcttcgcctg gcattcggcg gcgcggaaag cgctgaaatc
    10261 atggctgccg atcaggtgtt gcgcggcctg ctgcattgcc tgttcatcca gatgccggta
    10321 aaaccagcct acgcgccccc gcaggagccc cgggcgctgt gcacggttga gcagccaata
    10381 gacgtagcgc ctgccggtgg cggaaaagcg cgcgtgaaaa tccgggctca ctgcgcgcgc
    10441 ccagttcaca gcaatggcat cgggaagcag cgcgttgccg cccctgatcc atgcgctcgg
    10501 cgaacgctcg acatcaaagt cgaagtgcac cacctgcgcg agcgcatgca caccggcatc
    10561 ggtgcgtccg gcgcacgtcg tttcgatgtt cttgcccgcc acccccgaca gcgcgcgttc
    10621 gagcgcgtcc tgcaccgcgc aaccgtccgg ctgggtctgc cagccgcaga acgcagcgcc
    10681 gtcgtattcg agtccgagag caattctcaa agcgcgggtt ccgaagcggt tgccggtgcg
    10741 cagcaagagc gctgcgaatc aattgacggt ggatttgtgc ggcaaggcgc cgccacggga
    10801 aacggccgca gcggaaggaa cgtcaggaat cgagattggc gagcaccgtc tgcgcggcgg
    10861 ctttctgttc ggcgtcgcct tcctgaagca cttccttgag tatctcgcgt gcgccgtcct
    10921 tgtcgcccat ttcctggtac gccttggcga ggtcaaactt ggtctgcacg tcataccaac
    10981 gatcgtcctt gcccgcggct gaaacctggg gagagcttcc ggtgcccaga tcgaggttga
    11041 taccggacaa atcgatttcc tgcaccgcgc cggaatccgt cttgggtgcg ccggatgcgt
    11101 cgagcgagat cccgctcatg tcgaaatcca gtccgccgcc agcgggtttg ggttcctcgg
    11161 ccgcgggcac cgaggcagac ggcgcggcaa gggcgttgag gtcgaaatcg aaatcgatgg
    11221 tgttgccttc ctgcttcttc gacgcctcgg cttcctcagc agttgttcct gccggcggca
    11281 gttcgacgct gaaattcatc accggtgccg caccgggttc cagaatggtg ctgtccttat
    11341 gagactcgag cgtcgagggg tcgatgacct gggtcctgtc caactttctg ccgtcgtcgc
    11401 cgagatcgat gtcggtcgcg gtggtcgcgt tcgggtcttc gaagccgacg ttgaaatcga
    11461 cattatcggc ggctccgcct gcggcaactg ccgctaccgc agcggtttcg ccgccgctgg
    11521 gccggccggc tgcgtaacgc gggttgtccg gatcgatctg gtatcccagc gtgacggcct
    11581 ggtcccatag atatccgctg ccgccggttc ctttctgcaa ttcgcgcgcc agctgttcga
    11641 acgcgttcgt gtctttgcga ttggcataaa tctgcagcag cttgaggtgg atttcgtagc
    11701 gcgtgggaga tgccgcgagc gcttccttca gcagatcctc ggcctgggca tcacggccgt
    11761 aggcaaggaa aatctccgcc tccgcgaccg gatcgacttc ctcggcgatg tcgcttgccg
    11821 cgcgcgcgct ggccgcgctc aggtcggtgt cagtgctgtt gaacgcgggc gacgacgggg
    11881 ctgcagcgcc gactaccggc gcagggccag cttcttcggc ttcggccttc gattcccggc
    11941 ggcgtttgac ggcacgcacc gcgagcgcac cgatcagtac cagcacaccg agtccggcgc
    12001 cgagataggc gggttgctcc aacacccgat ccaacaggct gggctcgggt gccggtggcg
    12061 gcgcggattt gggcgcgggc ttcggtggcg gagcgggttg ggacggcggc gcggtctcga
    12121 ttttcggttg cggcgttgcc ggagccaccg gcgcctgggc cggagccggt ttggcctcgg
    12181 gtgctgatgc gggcgcaacg ggcacagtcg catgcgtttc cggcttcgcg gccggcttgg
    12241 gcgccggttg agccggcgcc ggttgtgccg gggttgtggc cgcagccgga ggcggggcag
    12301 ccggcttctg caggtcggcc atgcccttgt tcttgatctc cagcagggcc tgcaggtcct
    12361 tgatggtctt ttcgagcttg cttacgcgtt cgttggcatc cttgactgct ttgtcgcgtg
    12421 ccaccagttc ctcttccagc gcgcgcacgc gggcctgacc ctggcctgcc ttcgcgtctc
    12481 cggcgggagc cgcggccgcc gccgccggtt cgccttgcga aagcttcagc acttccttcg
    12541 gttgttggtt cgctgcggcg gcctgatcct caaccttcgc cgtaattttc ccggcggccg
    12601 cctgctgggg tggctctgcg gccggcttcg cttcgcccgc cttggccgcc agttgctcgc
    12661 ggtatgcatg ccagtcgcgt gcctgcacca gtacttcctt gcgcgcgtcg gcctgcgcga
    12721 cgctgccgta ctgatccggc ggcggcagct tgatgatctt tccggtcttc agccggttca
    12781 tgttcttgcc ggtgaaggca tccggattgc cccgcaacag cagcaccagc atttgttcca
    12841 cgctcacgcc cgcaggcttg gtggcaagcg cgatcttggc cagcgtatcg ccacgcgtca
    12901 ccgggccata gctgtcgcca gttgcagcgc ttgttgcagc gctgctgcgg gaggtgctgt
    12961 tgatattcga ttcggtgagg aaggtcgcca tcggcccgcc gatggtttct accggtgccg
    13021 atggggcggg ctcggtgact gcgggcgcct cggtcttcgc cggcgcctgc gccgctgcag
    13081 gagattccac ggcaggtgcg gcttccggag caggcgctgc agcagcgggc gcttgtgctg
    13141 gcggttcggg aatcggctcg ggctgtggct ccggcttggg tggggaagcg cgtacctggg
    13201 cttcggcggc cttctgccgg gcacgttcgg cgatcaacga tgggggatcg agcagcgcag
    13261 tgaactcgcg gctgatcctc ccggaagacc aggacaattc caccagcaaa tcgacgaagg
    13321 gctcgttgac cggttgagtg gtcgtcagcc gcaggtaggg ctcgccgttc gcgcgttttt
    13381 cgatgctgac cttggccttg tcgagcgcgt tcgagtaaag caggccggcg cgaccgaacg
    13441 cctccggagt ggcgagacgc gcagcgagcg aactgatttc ttttttctcg acgaacagct
    13501 cgatctccgc atcgagcggt tcaccgagcg ccgacgtgac gttgagcttg cccaaacctg
    13561 ctccgtgcgc tactgcagag acagcgagca acgacgcggc gaggatccat gcgcgaagag
    13621 acgttatgcc caaggcgccc accctgatcg tttttgtata aagggtaatt aaaataacat
    13681 catggattta gagatgcaag ctcaactttc ctcttacatt ctccggtaac ccaagacttt
    13741 ttcgatcaaa tttgaggtat cccccgggga cccaccgagg cgaaccatta ctgctcgagc
    13801 aggattcgca gcatgcgacg cagcggttcc gccgctcccc acagcaactg gtcgcccacg
    13861 gtaaatgcag ccaggtactc gccgcccatc ggcagtttgc gcaatcgccc gatcggcacc
    13921 atgagcgtgc cggtgaccgc cgctggcgtc aactcccgca tcgaagcctc acgctgattg
    13981 ggcaccacct tcacccactg attggctgat gccagcagcc cttcgatttc ttcgagtggc
    14041 agctccttcg tcagcttgat ggtgagcgcc tggctgtggc agcgcatggc gccgatgcgc
    14101 acgcacacgc cgtcgatcgg gaccggattg gcttcgcgtc ccaggatctt gttgccctcc
    14161 gcctgcgctt tccactcctc gcggctctga ccgttgccaa ggtccttgtc gatccacggt
    14221 atcaggctgc ctgccagcgg cagcccgaag tgctcgctcg gcaatgcgcg gctgcgcagc
    14281 gcgtcgctca ccttgcggtc gatttccaga atcgaggagg ccggatcttc gagcagcgat
    14341 tttgcggcgc catggatgta gcccatctgt gacaccagct cgcgcatgtt ctgggcgccg
    14401 gcgccggagg cggcctg
//
LOCUS       DASQOP010000147         3251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1067203, whole genome shotgun sequence.
ACCESSION   DASQOP010000147 DASQOP010000000
VERSION     DASQOP010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067203"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..1840
                     /gene="ggt"
                     /locus_tag="VE030_06065"
     CDS             104..1840
                     /gene="ggt"
                     /locus_tag="VE030_06065"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HZF23015.1"
                     /translation="MQNPRTGVRWMVAAITSISITAFAPGHAASVAPVTAENGMVVSA
                     QHLATMVGVDVLKRGGNAVDAAVAVGYALAVVYPAAGNLGGGGFMTIQLADGRKTFLD
                     FRETAPKGATANMYLDKDGNVIKGLSTKGHLAVGVPGSVSGMEYAREKYGTMKRADLI
                     APALQLAEDGFALDQGDVDMLLTATKDFQEDPASAAIFLNNGKPFQVGEKLVQHELAE
                     TLREISKRGTDGFYKGWVGSAIVASSQAGKGLITQEDLDGYKTRELAPVECDYRGYHV
                     VSAPPPSSGGVIICEILNVLEGYPLKELGYHSAEAVHYQIEAMRHAYVDRNSYLGDPD
                     FVKNPLDRLLDKNYAARIRAVIDPKQAAVSKDIKPGVEPHEGSNTTHYSIADKDGNAV
                     SVTYTLNDWFGAQVTAAKTGVLLNDEMDDFTAKIGVPNLYGLVQGEANAIAPGKRPLS
                     SMSPTIVTKDGKPVIVVGTPGGSRIITAVLQTMINAIDYDMNAQEAADMPRFHQQWLP
                     DLTNLENYALSPDTRKILEGKGHKFGPPQPANHLAVIIIGAPSLTGKPVGNNRFYGAN
                     DPRRNTGLAAGY"
     gene            complement(1921..2166)
                     /locus_tag="VE030_06070"
     CDS             complement(1921..2166)
                     /locus_tag="VE030_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23016.1"
                     /translation="MSIRIASVPPAVLFQSCLEHQIGVLLDWHRVMHEIEYLVKGFLR
                     VVLESNVMPCILGDQLSKSRELAPKNSFRACSVACLR"
     gene            complement(2297..3127)
                     /locus_tag="VE030_06075"
     CDS             complement(2297..3127)
                     /locus_tag="VE030_06075"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014752504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZF23017.1"
                     /translation="MSGNPSPRTPSQRIAWTTTIVASTLVFIFLMAPILAIMPLSFSS
                     GSYLTYPLPGLSLRWYEDFISSPRWMMSLKNSMIIGVASTVLSMVLGTLAALGLTQWK
                     SKFKPLVLAVVLSPVVVPGVITAVGLYFFFAPIGLTGSYLGLILAHTALATPFVVIPV
                     SATLQSFDMNLARAAASLGASPLYAFRRVILPLILPGLASGALFAFATSFDEVVIVLF
                     MAGPEQRTLPREMFSGIRENISPTITAAAVILTTVSVILLATMEGLRRRNERLKRNNA
                     "
BASE COUNT          671 a         1031 c         1049 g          500 t
ORIGIN      
        1 gccgaacggt gatgacccca tggtgcgcgg gcgcgcgccc tgatagacct atgtcttcaa
       61 tagcaaccta ggctgcgaag gccggcgttt ccgaggagct cccatgcaaa accccagaac
      121 aggcgtgcgg tggatggtcg ccgcgatcac gtcgatcagc atcacggcct tcgcgcccgg
      181 ccacgcggcg tccgtggcgc ccgtcaccgc cgaaaacggg atggtggttt ccgcccagca
      241 cttggccacg atggtgggtg tggatgtgct caagcgcggc ggcaatgcgg tcgacgcggc
      301 ggtagcggtt gggtatgcgc tcgccgtggt ctatccggcg gccggcaatt tgggcggcgg
      361 tggattcatg accatccagc ttgccgatgg ccgcaagacg ttcctggatt tccgcgagac
      421 ggcgcccaag ggcgccaccg ccaacatgta cctcgacaag gacggcaacg tcatcaaggg
      481 cctgtccacc aaagggcacc tcgccgtcgg cgtgcccggc agcgtttccg gcatggaata
      541 cgccagggag aagtacggca ccatgaagcg cgcggatctg atcgcgccgg ccctccaact
      601 ggccgaggat ggcttcgcac tcgaccaggg cgacgtcgac atgctgctca cggcgaccaa
      661 ggattttcag gaggatcctg cgtcagccgc catcttcctc aacaacggca agccgttcca
      721 ggtaggcgag aagctcgtgc agcatgagct cgccgagacc ttgcgcgaaa tcagcaagcg
      781 gggcaccgac ggcttctaca agggctgggt cggcagtgcc atcgtggcat cgagccaggc
      841 cggcaaaggc ctgatcacgc aggaggacct ggacggctac aagacccgtg agctggcacc
      901 cgtcgaatgc gactaccgcg gctaccacgt ggtctccgcg ccgccgccaa gctcgggcgg
      961 cgtgatcatc tgcgagatcc tgaacgtgct ggagggctat ccgctcaagg agctgggcta
     1021 ccactccgct gaggccgtgc actaccagat cgaagccatg cgccacgcct atgtcgaccg
     1081 caacagctat ctcggcgacc cggacttcgt gaaaaacccg ctcgaccgcc tgctcgacaa
     1141 gaattacgcg gccaggatcc gtgccgtgat cgacccgaaa caggcggccg tgtccaagga
     1201 catcaaaccg ggcgtcgagc cgcatgaggg cagcaacacc acgcactatt cgatcgccga
     1261 caaggacggc aacgcggtgt cggtcaccta cacactgaac gactggtttg gggcccaggt
     1321 gacggcggcc aagaccggcg tgcttctgaa cgatgaaatg gacgacttca ccgccaagat
     1381 cggcgtaccg aacctctatg gcctggtaca gggcgaggcg aacgccatcg cgcccggcaa
     1441 gcgtccgctg tcctccatga gcccgaccat cgtcaccaag gacggcaagc cggtgatcgt
     1501 ggtgggcacc ccgggcggga gccgcatcat cacggccgtg ctgcagacga tgatcaacgc
     1561 catcgattac gacatgaacg cgcaggaagc cgccgatatg ccgcgcttcc accagcagtg
     1621 gctcccggac ctgaccaatc tggagaatta cgcgctgtcg ccggacaccc gcaagatcct
     1681 ggagggcaag ggccacaagt tcggtccgcc gcaaccggcc aaccacctgg cggtaatcat
     1741 catcggcgcg ccgtcgctga cgggcaagcc ggtcggcaac aatcgcttct acggggcgaa
     1801 cgacccgcgc cgcaacaccg ggctggctgc cggctactga tcgtcatcgc ccgatccaac
     1861 aaggtggctt cggcgggtgc atctcatcgc gacgcgagcg cagcggacga cgcgacatcg
     1921 tcaacgcaag caggcgaccg aacatgcccg aaagctattc ttgggtgcca attcacgcga
     1981 tttcgacagc tgatcgccaa gaatgcaggg catgacgtta ctctcgagaa caacgcgcaa
     2041 gaaacctttc accagatact cgatttcgtg catcacgcgg tgccagtcca acagcacgcc
     2101 gatctgatgc tctaaacaac tctggaaaag taccgcgggc ggtacgcttg cgatccggat
     2161 gctcaagacg ccaagcttga atgtggctct ctgatcaaga tggcggctcg cggattggga
     2221 acgctcaaca gctttcagga gaagagccag cggacggacg gtgctcccgg tccgccgcaa
     2281 gggataagga cgccggtcaa gcgttgttgc gtttcagccg ctcgttgcgg cggcgcaagc
     2341 cttccatggt ggcgagcagg attactgaca ctgtcgtcag gatcaccgcc gccgcggtga
     2401 tggtcgggct gatgttctcg cggatgccgc tgaacatctc gcggggcagg gtgcgctgtt
     2461 ccggcccggc catgaacagc acgatcacca cctcatcgaa gctggtcgca aaggcgaaca
     2521 gcgcgcccga cgccaagccg ggcaggatca gcggcaggat cactcggcgg aaggcgtaga
     2581 gcggcgaggc gccgagcgag gctgcggcgc gcgccaggtt catgtcgaag ctttgcagcg
     2641 tcgcgctgac cgggatcacc acaaagggcg tcgccagcgc ggtgtgcgct aggatcaaac
     2701 ccaggtaact gccggtaagc ccgatcgggg cgaagaagaa gtacaggccg accgcggtga
     2761 tgacgcccgg cacgacgacc ggcgacagca cgaccgccag cacgagcggc ttgaacttgc
     2821 tcttccactg cgtcagaccc agcgcggcta gcgtgccgag caccatggac agcacggtcg
     2881 aggcgacgcc gatgatcatg ctgttcttca gcgacatcat ccagcgcggg gaactgatga
     2941 agtcctcgta ccagcgcagg gagaggcccg gcagcggata ggtcaggtag gagccggagc
     3001 tgaaggacag cggcatgatc gccaggattg gcgccatcag gaagatgaac accagcgtgg
     3061 aagcaacaat ggtcgtggtc cacgcgatgc gctggctggg cgtgcggggc gaagggttgc
     3121 cgctcaattc ttcattcctc ctgtgacttg ctggccatgg accagttttc cgtagacggc
     3181 ggcgagcagc agggtggcga gcagcagcac cgcgcccagc gccgaggcca ggccccagtt
     3241 gaccgtttcg g
//
LOCUS       DASQOP010000148         9758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1671029, whole genome shotgun sequence.
ACCESSION   DASQOP010000148 DASQOP010000000
VERSION     DASQOP010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9758
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1671029"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="VE030_06080"
     CDS             <1..1023
                     /locus_tag="VE030_06080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011884003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZF23018.1"
                     /translation="SPIPRGATASEALRNTLALAQRADKLGYKRYWLAEHHNMPGIAS
                     AATAIVIAHVAGGTRTIRVGSGGIMLPNHSPLVIAEQFGTLASLYPGRIDLGLGRAPG
                     TDMLTAQALRRTMEDSAEQFPQDVLELQSYFAAAQPGQHVRAVPGAGLEVPIWLLGSS
                     LFSAQLAAMLGLPFAFASHFAPDLMKQALEIYRTRFQPSRQLDRAYVMLGANVLAADS
                     DAEARRQFTSQQQSFINLRRGSPGQVPPPIDDIDSYWSPAEKAMVERSLAVSFVGSPE
                     TVQRGLAAFIESTRPDELMITAHVHDQPARLRSIELVSNVRENLASAERESEKAPREV
                     AANTKR"
     gene            1020..2057
                     /locus_tag="VE030_06085"
     CDS             1020..2057
                     /locus_tag="VE030_06085"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HZF23019.1"
                     /translation="MKLSELLGIELPIIQAPMAGAQGHALAVAVSNAGALGSIGCATL
                     GIDAMRGELAAIKAGTGGPFNVNFFCHVPPVPSTERESAWRAALLPYYRKYGIDPEKI
                     PAGPGRAPFSGEAADVLDEIRPAVVSFHFGLPPKELLVRVKRLGAKILSSATTVDEAL
                     WLEAHGVDAIVAQGVEAGGHRGMFLSDDLTTQAGTFALLPQVVRAVKLPVIAAGGIVD
                     AQGVAAAMELGAAGVQIGTAFLLCPEATISAVHRAALKSKAARHTALTNLFTGRPARG
                     IMNYVMKELGPINSAAPAFPLATSAIAPLRAKAEAEGSGDFSPLWSGQNASGCKEIPA
                     AQLTRELAAGL"
     gene            complement(2078..3511)
                     /locus_tag="VE030_06090"
     CDS             complement(2078..3511)
                     /locus_tag="VE030_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZF23020.1"
                     /translation="MGRFAEYEQYDALGLADLVRRKEVTPSELLEAAIERVEARNPAV
                     NAVVMKLYDYGKKAIAESLPDGPFRGVPLLMKDLTSPVAGVRMTRGSKFFADTPPSPA
                     DSEHVKRLKRAGLVIFGRTNTCELGLSLTCEPQLHGPTRNPWNVAHISGGSSGGAAAA
                     VAARMLPIAHASDGFGSIRAPAACCGLVGLKPTRSRNTFAPFAGEGLGGVSTEHAVSL
                     TVRDSAALLDATRGPGPGDPYVAPAPVEPYLKEVGANPGELRIAWTKRAPNGARIEAE
                     CLRLLAETVELCADLGHHVEERDPEIDGAAVVPTFLTLASANTVVNLASHPAAGRPAR
                     EAEVERITWLTAKMGEKISAPDYVRATQTAHRLGRQMAAFHSEWDVLLTPGLASPAVK
                     LGWLDMMMDDVDEYWRRVFAFSPFTVWFNLTGQPGLMLPLGQTSGGLPLAVQLVAPYG
                     GEATLFRLGAQLEAARPWFGRKPALAS"
     gene            complement(3680..5092)
                     /locus_tag="VE030_06095"
     CDS             complement(3680..5092)
                     /locus_tag="VE030_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HZF23021.1"
                     /translation="MDTQIVKRYLPWVVATALFMEQLDSTIVNTAIPAMATSLQVAPL
                     SLKAVVTSYILSLAVCIPISGWMADRFGTRRVFAIAVALFTLSSILCGLSVNVPMLVA
                     ARTLQGVGAAMMMPVGRLAIIRTYPKSELLMAMNFVIIPALIGPLLGPTVGGLIVHWL
                     SWREIFFVNIPVGLAALYLIHRYMPDYRGESTRPLDLVGLVLFGSGMALLSWLLEIFG
                     EHHIDATSAAFVSLISVALLAAYVFHARQAKYPLLRLGLFRVRTFRVSVAGGFITRLG
                     VGGLPFLLPLLYQIGMGLPAWQSGLLMMPTAAAAMGMKLIAPKVLGRHGFRQVLVVNT
                     LMIGLTICLFSLVTPSSPITLIVGLSLAVGFFNSLQFSAMNSMAYADTEARDSSMAST
                     IASSMQQLSMSFGLACGSLIAAWYLGDAPQTDGAAVTSALHYAFLTLGVLTALSSLSF
                     WTLHAEDGANVSRGALSEAP"
     gene            complement(5310..7022)
                     /locus_tag="VE030_06100"
     CDS             complement(5310..7022)
                     /locus_tag="VE030_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZF23022.1"
                     /translation="MNQEIAMVSELYEKRREQMFPKLTAMQISRLEAHGRRLRTQAGK
                     VLVEPGQRHHDLFVVLAGSLEIGLPGMRGEELVTVLLPGDFAGEMSTLRGVPGFTRIR
                     VRDSGEVLAISEENLRNVVQTDAEISETLMRAFILRRMGLVASGQSEAMLLGSRHSAS
                     TLRLREFLTRNSYPYVSVDVEADSGVQALLERFQVSVNEIPVLIGRCGRVFRNPSIEE
                     VADFLQMNPTIDDTRVHDLVVVGAGPAGLAAAVYAASEGLDVLVVEANAPGGQAGTSS
                     RIENYLGFPTGISGMALAGRALVQSQKFGAKVSVACHATRLRCDRRPYSLELSDGRAV
                     QASTVIVATGAKYRELAIADLPKFIGAGVYYAATHLEAKLCEGEEIVVVGGGNSAGQA
                     AVFLAGACRHVHILVRSGGLADSMSRYLIRRIEENPRITLHTRTQIMALEGNEHLERI
                     GWRNDATGQVENRDIGNVFLMTGAVPNTAWLEGCVALDNKEFVRTGSELQAEDLTAAG
                     WLLPRPPHLLETSLPGVFAVGDVRSGSVKRIASAVGEGSICVQFVHRALREISVPARQ
                     MAAA"
     gene            complement(7019..7285)
                     /locus_tag="VE030_06105"
     CDS             complement(7019..7285)
                     /locus_tag="VE030_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HZF23023.1"
                     /translation="MPAQCTHLNLVKTGRPNSDGCEECLATGDTWVHLRLCRTCGHVG
                     CCDSSRNKHATKHFHATRHPIITSLEPGEDWSWCYVDELELLLE"
     gene            complement(7314..7874)
                     /locus_tag="VE030_06110"
     CDS             complement(7314..7874)
                     /locus_tag="VE030_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HZF23024.1"
                     /translation="MSQHQKFELSVPVSEIDHALGPAHAPVTLVEYGDFECPICKQAA
                     PAVKLMLKHFSGRLRVVFRHFPQEEVHPHALQAALAAEAAGGQGKFWLMHDLLFENQS
                     HLKSQHLRSYAEQLELDMVRFDADMEDELYLQRIREHTEGGVLSGVRATPTFFINGWI
                     HDASFGMQSLMDGIDAALRTVPPSHK"
     gene            complement(8060..9202)
                     /locus_tag="VE030_06115"
     CDS             complement(8060..9202)
                     /locus_tag="VE030_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZF23025.1"
                     /translation="MTPLEKTVLPDGIRSRFVNDINGLNMHVLEAGFEVSNRPCVLLL
                     HGFPELAYSWRKVMLPLAQAGFHVVAPDQRGYGRTTGWDGDYGGDVGSFRMLNLVRDA
                     LGLVSALGYRSVKAVVGHDFGSPVAAWCSLLRPDVFRSVVLMSAPFAGPPALPFNTDN
                     DAAKPSAADIHEELAALRRPRKHYQWYYSTPEANDDMWRCPQGVHAFLRAYYHHKSAD
                     WRNNRPFPLKSWSAGELEKMPTYYIMDLDKGMAETVAPQMPSSSEIATNKWLPDEELA
                     VYSAEYRRNGFQGGLQWYRCMTGSQYIAELQTFSGRTIDVPSMFIAGKSDWGSHQVPG
                     ALEKMQNSACTQMRGCHLLDGAGHWVQQEQPEQVSTSLIRFLQSAH"
     gene            9359..>9758
                     /locus_tag="VE030_06120"
     CDS             9359..>9758
                     /locus_tag="VE030_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HZF23026.1"
                     /translation="MPSENTEPKDAADTPWQPSQRYPDSAVQIIDQSFARYRLNLAAV
                     ERIAHGFRWSEGPVWFGDGRYLLWSDIPNNRIMKWEEETGAVSIFRRPSNFANGNTRD
                     RQGRLVTCEHGGRRVTRTEYDGTISVIAGAF"
BASE COUNT         1785 a         3096 c         3130 g         1747 t
ORIGIN      
        1 tcaccgatcc ccaggggcgc gaccgcgtcc gaggcattgc gcaacacgct tgcgctggcg
       61 cagcgcgccg acaagctggg gtacaagcgc tactggctcg cggagcatca caacatgccg
      121 gggatcgcca gcgccgcgac cgcgatcgtc attgcccacg tggccggcgg caccaggacc
      181 atccgtgtgg gctccggcgg catcatgcta ccgaatcact cgcccctggt gatcgccgag
      241 cagttcggta cgctcgcctc actgtatccg gggcgcatcg acctgggcct gggccgggcg
      301 cccggtaccg acatgttgac ggcgcaggcg cttcgccgca cgatggagga cagcgcggag
      361 cagttcccgc aggacgtgct ggaactgcag agttatttcg ccgcggcaca accgggacag
      421 cacgttcgcg cggtgccggg tgcgggtctg gaggtgccga tctggttgct gggttcgagc
      481 ctgttcagcg cgcaactcgc ggccatgctc ggattgccgt tcgctttcgc ctcgcatttc
      541 gcgcccgacc tgatgaaaca ggcgctcgaa atctaccgca cccgcttcca gccctccaga
      601 cagttggacc gtgcttacgt catgctcggc gcgaacgtgc tcgccgccga tagcgacgcg
      661 gaagcgcgcc ggcagtttac ctcgcaacag caatcgttca tcaatctcag gcgcgggtca
      721 ccgggacagg tgccgccgcc gatcgacgac atcgattctt actggtctcc cgcggagaag
      781 gcaatggtcg aacgttcgct cgccgtgtcg ttcgtgggct cgccagagac ggtccagcgc
      841 ggtcttgcgg ccttcatcga atccacgcgg ccggacgaac tgatgatcac cgcacacgtt
      901 cacgatcagc cggcgcgtct gcgttcgatc gagctcgttt ccaatgtgcg ggaaaatctg
      961 gcttcagcgg agcgcgaaag cgagaaggcg ccgcgcgagg tcgcggcgaa tacgaaacga
     1021 tgaaactgtc cgaactcctc ggcatcgagt tgccgatcat ccaggcgccc atggcgggcg
     1081 cgcagggcca cgcgctggcg gtggcagtct ccaatgccgg cgctctcggc tcgatcggct
     1141 gcgccacgct cggcattgac gcgatgcgcg gcgaactcgc ggcgatcaag gcggggaccg
     1201 ggggaccttt caacgtaaac ttcttctgcc acgtgccacc cgtgcccagc acggagcgcg
     1261 aatcggcctg gcgtgcggcg ttgttgcctt actacaggaa gtacggaatc gatccggaaa
     1321 aaattcccgc cggaccgggc cgtgcgccct tcagcggcga ggctgccgac gtgctggatg
     1381 aaatcaggcc cgcggtggtg agctttcatt tcgggttgcc gccaaaggaa ctgctggtgc
     1441 gggtgaaacg cctgggcgcg aaaatactgt cctcggcgac caccgtcgac gaggcgctct
     1501 ggctcgaagc gcacggggtt gacgccatcg ttgcccaagg ggtggaggcg ggcgggcatc
     1561 gcggcatgtt cctgtcggat gatctgacca cgcaggccgg aacgtttgcg ttgctgccgc
     1621 aggtggtgcg cgcggtgaag ctgccggtca tagctgcggg cggcatcgtg gatgcgcaag
     1681 gcgtggcggc ggccatggaa ctgggcgcag ccggcgtgca gatcggaacc gctttcctgt
     1741 tgtgcccgga agccaccatc agtgcggtgc atcgcgcggc gctgaaaagc aaagccgcgc
     1801 gccacacggc gctcacgaac ctgttcacgg gccggcctgc gcggggaatc atgaactacg
     1861 tcatgaaaga gctcggcccc atcaacagcg cggcgccggc gtttccgctg gcgacctcgg
     1921 ccatcgcccc cttgcgcgcc aaagcggaag ccgaaggctc gggtgatttc tcgccgctct
     1981 ggtccggaca gaacgccagc ggctgcaagg agataccggc cgcgcagctc acgcgggagc
     2041 tggccgcagg actttagcca tcgattctgc gcactgctca ggacgcgagc gccggcttgc
     2101 gtccgaacca ggggcgcgcc gcttcgagct gtgcaccgag gcggaagagc gtcgcctcgc
     2161 cgccgtaagg cgcgacgagc tgtaccgcga gcggcagccc gcctgaggtc tggccgagcg
     2221 gaagcatcaa tccgggctgg ccggtgaggt tgaaccagac ggtgaaggga gagaacgcaa
     2281 acacccgccg ccagtattca tccacgtcgt ccatcatcat gtcgagccag ccgagcttca
     2341 ccgcgggcga agccagtccc ggcgtcagca gcacgtccca ttccgagtgg aaggccgcca
     2401 tctgccgtcc gaggcgatgc gccgtttgcg ttgcgcgaac gtagtcgggg gcgctgattt
     2461 tctcgcccat cttcgcggtc agccaggtga tgcgctccac ttcagcttcg cgtgccgggc
     2521 gtccggcagc cggatggctg gcgagattca cgacggtgtt cgccgacgcc agcgtgagaa
     2581 aggtcggcac gaccgccgcg ccatcgatct ccggatcgcg ttcctcgacg tggtggccga
     2641 gatcggcgca cagttccaca gtctcggcga gcaggcgcag gcactccgct tcgatccggg
     2701 caccgttggg cgccctcttt gtccacgcga tgcgcagctc gccggggttc gcaccgactt
     2761 ccttgagata gggttccacg ggcgcgggcg cgacataggg atcacccgga ccggggccgc
     2821 gcgtcgcgtc cagcagcgcg gcggaatcac gcacggtgag gcttaccgcg tgttcggtcg
     2881 aaacaccgcc cagtccttcg ccggcgaatg gagcgaaggt gttgcggctg cgcgtcggtt
     2941 tgaggccgac cagtccgcag catgccgcgg gcgctcggat cgagccgaac ccgtcagagg
     3001 cgtgcgcgat cggcaacatg cgggccgcga cggccgcagc tgcaccgccg ctcgagcctc
     3061 cggagatatg tgcgacgttc cagggattgc gcgtcggacc gtggagttgc ggctcgcagg
     3121 tcagcgagag accgagttcg caagtgttgg tccgtccgaa gatgacgagc cctgcccgct
     3181 tcaggcgctt cacgtgctcg ctgtcggcag gtgaaggcgg cgtgtcggcg aagaacttcg
     3241 agccgcgcgt catccggaca cccgcaaccg gtgaggtgag atccttcatc agcaagggca
     3301 cgccgcggaa cggtccgtcg ggaaggctct cggcgatcgc cttcttgccg tagtcgtaga
     3361 gtttcatgac tacggcgttg actgccggat tgcgcgcctc gacccgctcg atcgccgctt
     3421 caagcaactc ggagggcgtg acctccttgc gtcgcaccag atccgcgaga cccagcgcat
     3481 cgtattgttc gtattctgcg aatcggccca tttctttcct cccggaaagg ggcaagggta
     3541 aagggaaaag gtgaaaggga aaaggtgaaa gaggaaaggc gaaaggtgaa ggggaaaggg
     3601 gaaagggacc ggctagactt ttctcatttc tcatttctca tttctcattt ctcattcctc
     3661 gttcctcgta ccttgtctct caaggcgcct cgctgagcgc gccccggctg acattggcgc
     3721 catcctcggc gtgtaatgtc caaaaggata gcgatgacaa agccgtcagc acgcccagcg
     3781 tgaggaacgc atagtgcagg gcgctggtga cggcggcgcc gtctgtttgt ggtgcgtcgc
     3841 ccaggtacca ggcggcgatc aacgagccgc aagccaggcc gaaagacatc gagagttgct
     3901 gcatcgaact ggcgatggtg ctggccatgc tcgaatcgcg cgcctcggtg tcggcatacg
     3961 ccatcgaatt catggcggaa aattgcagag agttgaagaa gccgacagcc aggctcaggc
     4021 cgacgatcaa agtaatcggg ctggacggag tcaccaggga aaacaggcag atggtcaggc
     4081 caatcatgag cgtgttcacc accagtacct gcctgaatcc atggcggccg agaactttgg
     4141 gcgcaatgag cttcattccc atggcggccg ccgcggttgg catcatcagc aagcccgact
     4201 gccaggcggg caggcccatg ccgatctggt aaagaagcgg aagcaggaag ggaagtccgc
     4261 cgacgcccag ccgggtaatg aacccgccgg cgacggacac ccggaacgtg cggacccgga
     4321 aaagaccaag gcgaagcaac ggatacttcg cctgacgggc atgaaagaca taggcggcca
     4381 gcagtgcaac ggagatcaga gacacaaagg ccgccgaagt cgcatcgatg tgatgctcgc
     4441 cgaatatctc cagcagccac gacagaagcg ccatgccgct gccgaacagg accagtccca
     4501 ccaggtccag aggtcgcgta gactcgccgc ggtaatcggg catgtagcga tgaatgagat
     4561 agagcgccgc caggcccacc ggtatgttga cgaagaatat ttcccgccac gacagccagt
     4621 gcacgatcag gccgccgacg gtcggaccga gcagcgggcc gatcagcgcc gggatgatga
     4681 cgaagttcat cgccatcagc agctcggatt tcgggtaggt ccggatgatc gcgagcctgc
     4741 cgacgggcat catcattgcc gccccgacgc cctgcagcgt gcgtgcagcg accagcatgg
     4801 ggacgttcac ggacaagccg cagagaatcg aagaaagggt gaatagcgcc acggcaatcg
     4861 caaacacgcg ccgtgtgccg aagcggtcgg ccatccagcc gctgattgga atgcaaacgg
     4921 ccagactcag gatgtagctg gtgacgaccg ccttgagact gaggggcgcc acctgcaggc
     4981 tcgtggccat ggccgggatg gccgtgttca cgatggtgga atcgagctgc tccatgaaca
     5041 aggccgtggc gactacccac ggcaggtagc gcttgactat ctgggtatcc atgaggctcg
     5101 gcagagggga atctggcatt gcggttcggt caaatagtac cttacgtctt cgtaaggcct
     5161 gtttgcccgg cccccgcgcg acagcaccca gcgctgcttt tactttcctc ttttagcttt
     5221 tgactgcggc gtgttgtagt tccggcataa cttgaaagtt agcctccact gcaacaagcc
     5281 atacggcgtg ttgtctcttt cctctgcggc tacgccgccg ccatctgccg cgccggcacc
     5341 gaaatctccc gcagtgcgcg gtgtacgaac tgcacgcaga tcgatccttc acccaccgca
     5401 gacgcgatgc gcttcacgct gccggaacgc acgtcgccca ccgcgaacac gcccggcagg
     5461 ctggtctcca gcaggtgcgg cggtcgcggt agcagccagc ccgccgcggt caggtcttcg
     5521 gcctgcaact ccgatcccgt gcgcacgaat tccttgttgt ctagcgccac gcagccttcc
     5581 agccaggcgg tattcggcac ggctccggtc atcaggaaga cgttgccgat gtcgcgattc
     5641 tcgacctggc ccgtggcatc gttgcgccag ccgatgcgct ccagatgttc attgccctcg
     5701 agcgccatga tctgggtgcg ggtatgcagc gtgatccgcg ggttttcctc gatgcgccgg
     5761 atcaggtagc gcgacatgct gtcggccagg ccgcccgagc ggaccaggat atgcacgtgg
     5821 cggcaggcgc cggcgagaaa gaccgcggcc tggcccgcgg aattgccgcc gccgacgacg
     5881 acaatttcct caccttcgca cagtttcgct tcgagatgcg tggcggcgta atagacaccc
     5941 gccccgatga acttcggcag gtctgcaatg gccagctcgc gatacttcgc tcccgttgcg
     6001 acgatgacgg tactggcttg cacggcgcga ccgtccgaga gttccagaga atacggccgg
     6061 cgatcgcagc gcaggcgcgt ggcatgacag gcgacgctta ctttcgcccc gaacttctgc
     6121 gattgcacca gcgcgcggcc ggccaacgcc atgcccgaga ttccggtggg aaagccgaga
     6181 tagttctcga tcctggagct ggtcccggcc tggccgcccg gtgcgttcgc ttcgaccacc
     6241 agcacgtcga gcccctccga cgccgcatag accgcggcgg caagtcccgc cgggccggcc
     6301 ccgaccacga ccaggtcgtg gaccctggta tcgtcgatgg tcggattcat ctgcagaaaa
     6361 tcggcgactt cctcgatgct tggattcctg aacacccgtc cgcagcggcc gatgagcacg
     6421 ggaatctcgt tcaccgaaac ctggaagcgt tcgagcaggg cctggacgcc gctgtcggct
     6481 tcgacatcga cgctgacata gggatagctg ttgcgcgtca ggaattcgcg cagccgcagc
     6541 gtgctggctg aatggcgcga gccgagcagc atcgcttcgc tttgtcccga tgccaccagt
     6601 cccatgcgcc gcaggatgaa ggcacgcatc agagtctcgc tgatttccgc atccgtctgc
     6661 actacgttgc gcaggttctc ctcgctgatc gcgagaactt ctcccgagtc ccgcacgcga
     6721 atgcgggtga aacccggcac gccgcgcagc gtgctcattt ccccggcgaa atcacccggc
     6781 agcaggacgg tcaccagttc ctccccgcgc atgccgggaa gcccgatctc caggctgccg
     6841 gccagtacga cgaacaggtc gtgatggcgc tggcccggct cgaccagcac cttgccggcc
     6901 tgcgttcgca gacgccgccc atgcgcttcg aggcgggaaa tctgcattgc cgtgagcttg
     6961 ggaaacatct gctcgcggcg cttttcatag agttcgctga ccatcgctat ctcctggttc
     7021 actccagcag caattccagt tcatcgacgt agcaccagct ccagtcctcg cccggttcca
     7081 gcgaggtgat gatcggatgc cgggttgcgt ggaagtgttt ggtggcgtgc ttgttcctcg
     7141 agctgtcgca gcaaccgacg tggccgcagg tgcggcacag gcgcagatgc acccaggtgt
     7201 cgcccgtggc gaggcattcc tcgcagccgt cgctgttcgg ccggccggtc ttcacgaggt
     7261 tcagatgggt gcattgagct ggcatggcta gcgatccggc ggttttggtt tggctacttg
     7321 tgcgacggcg gcaccgtgcg caaggcggca tcgatgccgt ccatcaggga ttgcatgcca
     7381 aaggacgcgt cgtgaatcca tccattgatg aagaacgtgg gcgtggcgcg cacaccgctc
     7441 agcacgccgc cttcggtgtg ctcgcggatg cgctgcagat agagctcgtc ttccatgtcg
     7501 gcgtcgaagc gcaccatgtc gagttcgagc tgttcggcgt agctgcgcag gtgctgggat
     7561 ttcaggtggc tctgattttc gaacaggagg tcgtgcatga gccagaactt gccctggccg
     7621 cccgccgcct ccgcggcgag cgcggcctgc aaggcatgag gatgcacttc ttcctgcggg
     7681 aaatggcgga agaccacgcg cagcctgccg gagaaatgct tcagcatcag cttcactgcc
     7741 ggcgcggcct gcttgcaaat tggacactcg aaatcgccgt attcgaccag cgtgacgggg
     7801 gcgtgcgccg gcccgagcgc atgatcgatc tcgctcaccg gtaccgacag ttcaaatttt
     7861 tgatgttggc tcatgagttt ttgcgtgcat gtctggttgc ccattgtacc gagtagtcgg
     7921 ctacttcctc ccttctaggc tccacgcaga gaaagtgcgg gcggccgggg aatgacttca
     7981 cggccgtgac gtaccgcgat ttgcgcccgg cctgaaacga aaagggctcg ccgaaggcta
     8041 gattagagga atcagggggt cagtgtgcgc tttgcagaaa ccggatcagc gaagtgctca
     8101 cttgctccgg ctgctcctgc tgcacccagt gtcctgcacc gtcgagcaga tggcagccgc
     8161 gcatttgcgt gcatgcgctg ttctgcattt tctccagcgc accgggcacc tggtggctgc
     8221 cccagtcgct tttgccggcg atgaacatcg acggcacatc gattgtgcgg cctgagaacg
     8281 tctgaagttc ggcgatgtat tggcttccgg tcatgcaccg ataccactgc agtccgccct
     8341 ggaacccgtt cctgcggtac tccgcgctgt acacggcgag ctcctcgtcg ggaagccatt
     8401 tgttcgtcgc aatctcggaa ctggagggca tctggggcgc cacggtctcc gccatcccct
     8461 tgtccagatc catgatgtag taggtcggca ttttctccag ttcgcctgcg ctccaagact
     8521 tgagcggaaa gggtctgttg ttcctccagt ccgcactctt gtggtggtag taggcgcgca
     8581 ggaaggcgtg aacaccctgt ggacatctcc acatgtcgtc gttggcctcg ggcgtggagt
     8641 agtaccactg gtaatgcttg cgcgggcggc gcagtgccgc cagttcctcg tggatgtcgg
     8701 cggcggacgg cttcgctgca tcgttatcag tgttgaacgg taacgccggc gggcccgcga
     8761 acggcgcgct catcagcacc accgagcgga acacgtccgg ccgcagcagt gaacaccagg
     8821 ccgcgaccgg agagccgaag tcgtgcccga ctaccgcctt cacactgcgg tatccgagcg
     8881 cggagaccag cccaagcgca tccctgacca ggttgagcat gcggaaggaa ccgacgtcgc
     8941 caccgtagtc gccgtcccaa ccggtggtgc gcccatagcc gcgctggtcc ggcgcgacca
     9001 cgtgaaagcc cgcctgagcc agcggcagca tcaccttcct ccagctatac gcaagctccg
     9061 gaaagccgtg cagcagcagc acgcagggcc ggttactgac ttcgaacccg gcctccagca
     9121 cgtgcatgtt gagaccattg atgtcgttca cgaaccggga gcgtatgccg tcagggagaa
     9181 cagtcttttc aagtggagtc atcttgtcga ttttcgtctg caagaatgtt tgacggatat
     9241 taccggatca ctgtgccgca gtttcttccg gacgacctct ctcggttgcc cgcgggtggt
     9301 tgctgcgggc tgccgcgctg taaactgcac gaaaactctc cacggaaccg gaacatctat
     9361 gccatctgag aacactgaac ccaaagatgc tgccgatacg ccctggcagc ccagccagcg
     9421 ctacccggat tccgcagtcc agatcatcga ccagagcttc gccaggtacc gcctcaatct
     9481 cgcggcggtc gagcggatcg cccacggctt tcgctggagc gagggaccgg tgtggttcgg
     9541 tgacgggcgt tatctgctat ggagcgacat ccccaacaac cggatcatga agtgggagga
     9601 agaaaccggc gccgtgagca tttttcgccg gccttccaac ttcgccaacg gcaacacgcg
     9661 cgaccggcag gggcggctcg tcacctgcga gcacggcggg cggcgcgtca cgcgcaccga
     9721 gtacgacggc acgatctcgg tgatcgccgg cgccttcg
//
LOCUS       DASQOP010000149         8142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2675467, whole genome shotgun sequence.
ACCESSION   DASQOP010000149 DASQOP010000000
VERSION     DASQOP010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8142
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2675467"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /locus_tag="VE030_06125"
     CDS             complement(<1..1152)
                     /locus_tag="VE030_06125"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HZF23027.1"
                     /translation="MDATILLIFAVVYLGMILGGLPFLQLDRTGIALLGAIALIGVGA
                     LSLGEAWAAVHAPTLILLFAFMVVSAQLRLGGFYTWVTHKTGNLPLPPAQLLGALIVI
                     VAGLSAVFSNDIICLAMAPVLIDVCLARRLNPVPFLLALACSANVGSALTLIGNPQNM
                     LIGEKLHLSFSGYLLEAAIPVVFGLAVVWGLIAWRSRGSWELEPAEPATAAVPPHPEP
                     PPPVDRWQSAKGIAVAAAAFLLFLFAPLPREVVALTAAGVLMMSRKLHSHKMLGLVDW
                     QLLVLFVGLFVVNHALDKTGVTAQAVGRLAALGVDLHQPGPLFVAAFLLSNVVSNVPA
                     VMLLLPVATHELAGLLLALASTLAGNLLIVGSIANIIVVDAAARRGIRID"
     gene            1364..1726
                     /locus_tag="VE030_06130"
     CDS             1364..1726
                     /locus_tag="VE030_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23028.1"
                     /translation="MQIKYFAAALVLMSFLGAESRAQEGPVTWSDPTCGFFVVQLPPG
                     DPADAFGLYSWKVHPGPQVGDVVEGENMAAAKELVLVNQRSGDKHVVIHWANAKAAEM
                     LIRNTPVQCASKWKKKKK"
     gene            1749..2327
                     /locus_tag="VE030_06135"
     CDS             1749..2327
                     /locus_tag="VE030_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23029.1"
                     /translation="MNPATVLLIVLGAIFGPVYLAYCEYLSGETAETHTLTERADRWE
                     LPDGSILRLRGGRGYRPVALELTPDLNLYRFRFTFDVARSRASPKDPDNEYQVSVLQG
                     DVGIYERSFRAAGDGAVSVALDPMEILYSGSYVLLLEEVGAPRLQVAGVKLEVLNKVE
                     KKRTWLVWCGLVMLLVGAAIMVRDLVMHTLKK"
     gene            2324..2983
                     /locus_tag="VE030_06140"
     CDS             2324..2983
                     /locus_tag="VE030_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HZF23030.1"
                     /translation="MKAFDPDQFHGLSTAVLSDVMDVLGLMNQAMRPFVRPLDENLVL
                     AGRARTGLYMLAYEVREGENPYEVEIALVDDLKPGDVPVLACNGPTQRIAPWGELLST
                     AAQARGAAGCITDGLVRDVKQIREMRFPVFHGGIGPLDTKGRARMVQRDGPVECAGVK
                     VSNGDILFGDVDGVVVIPQERAAEVLAKAKEKVAGEGRTRDELRQGRLLREVFEKYRI
                     L"
     gene            3017..3574
                     /locus_tag="VE030_06145"
     CDS             3017..3574
                     /locus_tag="VE030_06145"
                     /inference="COORDINATES: protein motif:HMM:NF018949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HZF23031.1"
                     /translation="MYWLIAGLVIFFGVHIFSDFRSAREKTIQRLGEKRYKAAYSLLA
                     VAGFILIVIGVGKSEPVLLWAPAEWGRYAAVWFMPFAFISLAAAFIPSNFRRFTAHPM
                     LWSVTLWALLHLLANGNLTGLILFGAFGLYAVHAMSSQNARGARPSQFRRPLINDIGV
                     VVAGLILYWLFLHFHARLFGVPAVY"
     gene            3611..4054
                     /locus_tag="VE030_06150"
     CDS             3611..4054
                     /locus_tag="VE030_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23032.1"
                     /translation="MAKPIPPVLLTYVEGLKTHDIRRIAGTASGELTFISATRMLDKP
                     QFLKMLTALYTGFPDWNYAYDGIEDRGQGNYAIKWRQGGTHTAIWAMPGMDPIPPTGR
                     KVSIPEHYFFYRVSGDKLTLIFPEPIPGGAPRGILEQIGVKLPPL"
     gene            complement(4297..5769)
                     /locus_tag="VE030_06155"
     CDS             complement(4297..5769)
                     /locus_tag="VE030_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HZF23033.1"
                     /translation="MSERCGNSCDHDHVAEEVQETAREAPQVDESRRSFVCGALASGG
                     AAVSAATMTIPYVSTAQAQGLDPVKSGVRNHYYVAATDKSVHWGYFSKRLKPIVQIAS
                     GDFVTIEAITHHAYDDFERMIKGDPGVESIFYWDKNKKNVDRRGAGPMDASVLGRGAG
                     EGFGVHVCTGPVYVKGAEYGDILEVRIVDVSPRPCANPAHKGKSFGSNAAAWWGFHYN
                     DLIEEPKKREVITIYELDSSKERNWAKAVYNYRWVPQTDPFGVVHKIIDYPGVPVDHS
                     LVKENHGILKDVRIPVRPHFGVIGLAPKEADIVDSIPPGPFGGNIDNWRIGKGATMYY
                     PVQVDGALFSVGDPHASQGDSELCGTAIESSLTGTFQLILHKKATLVGSSLDDLKYPL
                     LETQDEWVLHGFSFANYLEELGPEAQSKIYSKSSIDLAMRDAFRKMRHFLMTTQGLTE
                     DEAISLMTVGVDFGITQVVDGNWGVHGIVKKSLFAGRTDF"
     gene            complement(6092..6586)
                     /locus_tag="VE030_06160"
     CDS             complement(6092..6586)
                     /locus_tag="VE030_06160"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZF23034.1"
                     /translation="MIRPCKDTDFAAIFEIVNDAAQAYRGAIPADCWQDPYMPEQELR
                     HELASGVVFWGYEENGELAGVMGIQDVADVTLIRHAYVRTARRRRGIGAALLFHLRKL
                     AEKPILIGTWKAATWAIHFYSKHGFRVLPEEEKNRLLRKYWTIPDRQIKTSLVLAEVQ
                     RPLR"
     gene            complement(6706..7230)
                     /locus_tag="VE030_06165"
     CDS             complement(6706..7230)
                     /locus_tag="VE030_06165"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZF23035.1"
                     /translation="MTAEAWVGQAQAAVARMKKPGVSSLDELRSRSGLEFLRDIAAGV
                     LPLPPIGKVLNFFLLEADEGRVVFQGTPTLDFYNPIGTVHGGWAATLLDSCMACAVQT
                     TLKKGQGYTTAELKLNLVRPLTDQTGPVRAEGKVIHAGRQIATSEGRLFGPDGKLYAH
                     GTTTCMIFQIPGPA"
     gene            7419..7706
                     /locus_tag="VE030_06170"
     CDS             7419..7706
                     /locus_tag="VE030_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23036.1"
                     /translation="MRIFVSAGFAVLLAVAAPAWGAEPEYDGQCAMSMAEGTGISTDC
                     AVVWLSPEGKIYCFFNQAAKEKFLLAPQENLQRAQAFWDNPAVLKQIRRQQ"
     gene            complement(7948..>8142)
                     /locus_tag="VE030_06175"
     CDS             complement(7948..>8142)
                     /locus_tag="VE030_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HZF23037.1"
                     /translation="LLRVVVPDIQVLEDFILNFLSRVPGVGNIKSSFALKQVKYQTAL
                     PLPAAAGTGREARATRGKKR"
BASE COUNT         1575 a         2527 c         2492 g         1548 t
ORIGIN      
        1 gtctatgcgg atgccgcgcc gcgccgcggc gtccacaacg atgatgttgg cgatgctgcc
       61 gacgatcagc aggttccccg ccagcgtgct cgccagcgcc agcaacaacc ccgccagttc
      121 gtgtgtggcc accggcagca gcagcatcac cgccggcacg ttggaaacga cgttggaaag
      181 gaggaaggcg gcgacgaaca acgggcccgg ctgatgcagg tccacaccca gcgccgccag
      241 gcgcccgacc gcctgcgcgg tgactccggt cttgtccagc gcgtgattca ccacgaacaa
      301 gccgacgaac agcaccagca actgccaatc caccagcccg agcatcttgt gcgaatgcag
      361 cttgcggctc atcatcagca cgccggcggc ggtgagcgcc accacttccc gcggcagcgg
      421 cgcgaacagg aacagcagga acgccgctgc cgcgacagcg atgcccttgg cgctttgcca
      481 gcgatccacc ggcggcggcg gttccgggtg cggcggcacc gccgcggtgg ccggctcggc
      541 cggctcgagt tcccacgacc cgcggctgcg ccacgcgatc agcccccaga ccaccgccag
      601 cccaaacacg accggaatcg ctgcctccag cagatagccg gaaaacgaca aatgcagctt
      661 ctcgccgatc agcatgttct gcgggttgcc gatcagcgtt agcgcagagc caacgttggc
      721 gctgcaggcg agtgcgagca gaaagggaac cggattgagc cgtcgtgcca ggcacacgtc
      781 gatcagcacc ggcgccatcg ccaggcagat gatgtcgttg ctgaacaccg cggacaggcc
      841 ggcgacgatt acaatcaacg cgccgagcag ttgtgccggc ggcagcggca ggttgccggt
      901 cttgtgcgtc acccaggtat agaagccgcc caggcgcaat tgcgcggaca ccaccatgaa
      961 ggcgaacagc aggatcagag tgggcgcgtg caccgctgcc catgcctccc cgaggctcag
     1021 cgcgccgacg ccgatcagag cgatcgcgcc cagcagcgcg atgccggtgc ggtccaactg
     1081 caggaaaggc aggccgccca gaatcatacc caggtaaacg actgcgaaaa tcaggagaat
     1141 ggttgcgtcc atttttgttc ttcgcgttcc cggccgagcg caagtctacg cgaactgcat
     1201 cgggctgcgc acgcgccgac agaccggatt gcctggctgt cccgcaggga ttgcaccctt
     1261 gcgcgaacgg cctccaattc aatttggaat tgtcggataa tctccttggg cggcgctgca
     1321 gccgtcccta acaagaatgc cggtaaataa aagggagaac gatatgcaga tcaaatactt
     1381 tgcggctgcg ctggtgctga tgtcctttct gggcgcggag tcccgggcgc aagagggccc
     1441 ggtgacctgg agcgatccga cctgcggttt tttcgtggtg cagttgccgc ccggagatcc
     1501 cgccgacgcc ttcggactgt attcctggaa ggtgcacccg ggtccgcagg tcggcgatgt
     1561 cgtcgaaggc gaaaatatgg ctgccgcgaa ggaactggtg ctggtgaacc agcgtagcgg
     1621 cgacaagcac gtggtcatcc actgggcgaa cgccaaggcg gcggaaatgc tgatccgcaa
     1681 cactccggtt caatgcgcaa gcaagtggaa aaagaaaaag aaatgacgcg gttcgccgtg
     1741 gcagcggcgt gaacccggcc acggtgctgc tgatcgtcct cggcgctatc ttcggtcccg
     1801 tttatctggc ctactgcgag tacctgagcg gcgagaccgc ggaaacgcat acgctcaccg
     1861 aacgtgcaga tcgttgggaa cttcccgatg ggtcgatcct gcgcttgcgc ggaggacggg
     1921 gttaccggcc ggttgcgctg gaactcacgc cggacttgaa cctttaccgc ttccgcttca
     1981 ctttcgacgt cgcgcgcagc cgagcgtctc cgaaagaccc ggacaacgag tatcaagtga
     2041 gcgtgctgca aggtgatgtc ggcatttacg agcgcagctt ccgcgcggcc ggcgacggcg
     2101 cggtttccgt cgcactcgac ccgatggaga tcctctacag cggcagctat gtgctgctgc
     2161 tcgaggaagt cggcgcgccg cggcttcaag ttgcgggcgt gaagctggag gtgcttaaca
     2221 aggtggagaa aaagcgcacg tggctggttt ggtgcgggct ggtcatgctt cttgtcggcg
     2281 cggcaatcat ggttcgtgat ctcgtgatgc atacactgaa aaaatgaagg ctttcgaccc
     2341 cgatcagttt cacggcctga gtaccgcggt gctctcggac gtcatggacg ttctgggcct
     2401 gatgaaccag gcgatgcgac cgttcgtccg cccgctcgac gaaaacctgg tgctggccgg
     2461 acgcgcccgc accgggttgt acatgctcgc ctacgaagtg cgcgagggtg aaaatcccta
     2521 cgaagtcgaa atcgcactgg tggacgatct caaacccggc gacgtgccgg tgctcgcctg
     2581 caacggcccg actcaaagaa ttgcaccctg gggtgaactg ctgtccaccg cggcgcaggc
     2641 gcggggcgcg gccggttgca tcaccgatgg cctggtgcgc gacgtgaagc agatccgcga
     2701 gatgcgtttc ccggtcttcc acggcgggat cggtccgctc gataccaagg gccgagccag
     2761 gatggtgcag cgcgacgggc cggtcgaatg cgccggcgtg aaagtgagta acggcgacat
     2821 tttgttcggc gacgtggatg gcgtcgtggt cattccgcag gaacgcgccg ccgaagttct
     2881 cgcaaaggca aaggagaaag tggcgggaga gggccgtacg cgcgacgagc tgcggcaggg
     2941 ccgtctgctg cgcgaggtgt tcgagaagta ccggattctc tgagccgccc cgacaccgac
     3001 gaaaggagaa tgaataatgt actggctgat cgctggactg gtcattttct tcggggtaca
     3061 tatcttctcg gatttccgca gcgcgcgcga aaaaacaatc cagcgtctgg gcgagaagcg
     3121 atacaaggcg gcttactcgc tgctggccgt tgccggcttc atactcatcg tgatcggcgt
     3181 cggcaagtcc gagccggtgc ttttgtgggc gccggccgaa tgggggcgtt acgccgcggt
     3241 atggttcatg ccgttcgcct tcatttcgct ggcggctgcg ttcatcccga gcaacttccg
     3301 gcgcttcacg gcgcatccca tgctgtggag cgtcacgctt tgggccttgc tgcacctgct
     3361 tgccaacggc aatcttaccg gcctgatcct gttcggcgcc ttcgggttgt acgcggttca
     3421 tgcgatgtcc tcgcagaatg cccgcggtgc gcggccgtcg cagttcaggc gcccgctgat
     3481 caacgacatc ggagtagttg ttgcgggttt gattctgtac tggctgttcc tgcatttcca
     3541 cgccagactg ttcggcgttc cggctgttta ctgaccaaaa tcgataacga caacagggag
     3601 gaaaaacgcc gtggcaaagc cgatcccgcc ggtgttgctg acctatgtcg agggactgaa
     3661 aacgcacgac atccggagga tcgccggaac cgcgtccgga gagctgacgt tcatctcggc
     3721 gacgcgcatg ctcgacaagc cccagttcct gaagatgctc accgcgctct acaccgggtt
     3781 cccggactgg aactacgcct acgacgggat cgaggaccgc ggccagggta attacgcgat
     3841 caaatggcgc cagggcggca cccacaccgc aatatgggcc atgccgggaa tggatccgat
     3901 cccgcccacg ggcaggaaag tcagcatccc cgagcattac ttcttttacc gcgtctccgg
     3961 cgacaagctg acgctcatct tccccgaacc gattcccggc ggagcgccca ggggaattct
     4021 ggaacagatc ggcgtaaagc ttccgcctct gtaaggcaac cgcgcttacc gcgctgaaaa
     4081 atcatacttc tcaccgcgaa tacacgaaga acacgaagga acacaaggaa accaaaaaga
     4141 agacgggcaa gaaaataaag tttcagcgat gattcacggc cagatcgttt tgctgccaat
     4201 acccgatatt agaaacaaga agttgttctt cttcgtgaac cttcgtgtct tcgtgtcttc
     4261 gtgtcttcgt ggtgatattt tgttagttga gatcgctcaa aagtcggtgc gtccggcgaa
     4321 cagcgatttc ttgacgatgc cgtgcacgcc ccagtttccg tccaccacct gggtgatgcc
     4381 gaagtccaca cccacggtca tcaacgaaat cgcctcgtct tccgtcagtc cttgtgtagt
     4441 catgaggaaa tgccgcatct tgcggaaggc gtcgcgcatc gccaggtcga tcgaggattt
     4501 cgaatagatc ttcgactgcg cctccggacc cagttcctcg agataattgg cgaagctgaa
     4561 gccgtgcagg acccattcgt cctgggtttc gagcaacggg tatttcaggt catcgagcga
     4621 ggagccgaca agcgtcgcct tcttgtgcaa gatcagctga aaggtgccgg tcagcgacga
     4681 ttcgatggcg gtgccgcaca gctcggaatc gccctgcgaa gcgtgcgggt cgcccacgga
     4741 gaacagcgcg ccgtccacct gcaccgggta gtacatggta gcgcccttgc cgatgcgcca
     4801 gttgtcgatg ttgccgccga acggcccggg tgggatcgaa tccacgatgt cggcttcttt
     4861 tggcgccagg ccgatcacgc cgaaatgtgg ccgcacggga atgcgcacgt ccttcagtat
     4921 cccgtggttt tccttcacca ggctgtgatc caccggcacg cccggatagt cgatgatctt
     4981 gtggaccacg ccgaacgggt ccgtctgcgg cacccagcgg tagttgtaaa ccgcctttgc
     5041 ccagttgcgc tccttcgacg aatccagttc gtagatggtg atgacttcgc gcttcttcgg
     5101 ctcctcgatc agatcgttgt agtgaaatcc ccaccacgca gcggcgttgc tgccaaagct
     5161 cttccccttg tgagccggat tcgcgcacgg acgcgggctg acgtcgacga tgcgcacttc
     5221 gagaatatcg ccatactccg cccctttcac gtacaccgga ccggtacaga cgtgtacgcc
     5281 gaagccttct cccgcgccac gccccagcac cgatgcatcc atcggccccg cgccgcgccg
     5341 gtcgacgttc tttttattct tgtcccagta gaagatgctc tcgacacccg gatcgccctt
     5401 gatcatgcgc tcgaaatcgt cgtacgcatg gtgggtaatc gcttcgatgg tgacgaagtc
     5461 gccggaggca atctgcacga tgggcttgag cctcttgctg aagtaacccc agtgcacgct
     5521 cttgtcggtg gcggcgacgt agtagtggtt gcgcacgccg gacttgaccg gatccagacc
     5581 ctgcgcctgc gccgtggaaa cgtacggtat ggtcatcgtt gcggcggaaa ccgccgcgcc
     5641 gccggacgcc agcgcgccgc acacgaagct tctcctgctt tcgtcgactt gcggcgcttc
     5701 ccgcgcggtt tcctggactt cttcggcaac gtggtcgtga tcgcatgaat taccgcaacg
     5761 ctcagacatg aattcttctc cggttaagtg aacgcgtcgc ctgaaatcat cgctcaactg
     5821 gacacgatga atcgccgggg cgccgatccg ttgatcatct cgcgaaatcg caataatcaa
     5881 gccagcacgc gcgtggccca ttgccgggga gaaaaaacac ctggtaacag aggaaatccg
     5941 aattgaatcg cgaacttgca ggaatgcctg gaggggacgg cggaacggga agacgctgag
     6001 aactcgggca ccggcatcga gcgcagcaaa ggccgcagcg cctgatgatt gtgcatcggc
     6061 gcggacgccg gggtgcgtcg cgcggccggg cttaacgcag cggccgctgc acttccgcca
     6121 gcaccagcga ggtcttgatc tgacgatccg gaatcgtcca gtacttcctg agaaggcgat
     6181 tcttttcttc ttccggtagc acgcggaaac catgcttact gtagaagtgg attgcccagg
     6241 ttgcggcttt ccaggtgccg atcaggatcg gcttctcggc aagcttgcgc agatggaaga
     6301 gcagcgcggc gcctatcccg cgacggcggc gtgcagtacg cacgtaggca tggcggatca
     6361 gcgtgacatc ggcgacatcc tgaataccca tcacgccggc cagttcgcca ttctcctcgt
     6421 agccccagaa caccacaccg ctcgcgagtt catgccgcaa ttcctgttcc ggcatgtatg
     6481 gatcttgcca gcagtcggcc ggaatcgcgc cgcggtaggc ttgcgcggcg tcgttgacga
     6541 tctcgaagat ggcggcgaaa tccgtatcct tgcaagggcg aatcattgtt acgggtgacg
     6601 agtcaggagt gaggagtgag gggcaaagcc tagtcgattt attgtcgcgt ttccgtttcc
     6661 tgtcaaggct ccaaggtccc ggttcctcga aagagcgcgt tgcgctcaag ctggaccggg
     6721 aatctgaaaa atcatgcacg tcgtggtgcc gtgggcatag agcttgccgt cgggaccgaa
     6781 caggcgccct tccgaggtgg cgatctgccg gcccgcgtgg atgaccttgc cttcggcgcg
     6841 caccggcccg gtctggtcgg tcagcggacg cacgaggttc agcttcagtt cggcggtggt
     6901 gtagccctgg ccttttttca gcgtcgtctg caccgcgcag gccatacagg agtctagcag
     6961 cgtcgcggcc cagccgccgt gcacagtgcc gataggattg tagaagtcca acgtcggtgt
     7021 tccctggaac accacccgcc cttcgtccgc ttccagcaag aaaaaattca acactttgcc
     7081 gatcggcggc agcggcagca cgcccgcggc gatgtcccgc aggaactcca gccccgaacg
     7141 gctgcgcaat tcgtccagcg agctcacgcc cggcttcttc atccgcgcca ccgcggcttg
     7201 cgcctgcccg acccatgctt ctgccgtcat cttcccatcc ttatctgctt ggtccgttag
     7261 cggataatgc ccccaagggg cgcctcgtcc gttgcggccg tgacgcggac aactgaccgc
     7321 gccttgtgcc ttcagtatca tgtttggcca gcgtaacgca gccgggctgc ccgaaaaagg
     7381 ggcttgacgc cgggttgtcg atttgcgaag gagtgggaat gagaattttc gtgtccgccg
     7441 gtttcgccgt tctgctcgcg gtggcggcgc cggcatgggg cgcagaaccc gaatacgacg
     7501 gccaatgcgc gatgagcatg gccgaaggaa ccgggatttc caccgactgc gccgtcgtct
     7561 ggctctcgcc cgagggcaag atctactgtt tcttcaatca ggccgccaaa gagaagttcc
     7621 tgctggcgcc gcaggagaat ctgcagcgcg cgcaggcatt ctgggacaat ccggccgttc
     7681 tgaaacaaat cagacgccag caataacgcg aggcttcgaa ccgcgtacgg gcgcaggtta
     7741 atcggcgcct cacctgccgc ctcctgccgg gtcgcggccg caacgtttca cgagttcacg
     7801 aggcactagg cgtcaggcac gaggcgcgag acaagcacca atcaagtcgc cgccaatact
     7861 ctcgtttttc cctctcgctc tctcttgctt ttcctccgcg tcctctgcgt tgaacgcccc
     7921 taacgccggc ccttggtcgg cctacgctca gcgcttctta cccctcgtgg ctcgcgcctc
     7981 gcgcccggtg cctgcggccg ccggcagcgg cagcgccgtc tggtatttca cctgcttcaa
     8041 cgcgaagctc gacttgatgt tgcccactcc aggcacgcga gacaggaagt tgaggatgaa
     8101 gtcttcgaga acctggatgt cgggcaccac tacgcgcaac aa
//
LOCUS       DASQOP010000150        15577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2479926, whole genome shotgun sequence.
ACCESSION   DASQOP010000150 DASQOP010000000
VERSION     DASQOP010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 15577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15577
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2479926"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VE030_06180"
     CDS             <1..366
                     /locus_tag="VE030_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZF23038.1"
                     /translation="VHLPYALARKYPRAGYEWIWQYVFPSKNRSADPDDGVIRRHHLD
                     ESVLQRAVKKAVRIVGISKLVHCHTFRHSLATHVLQSGYDIRTVQELLGHSDVSTTMI
                     YTHVLNRGGRGVISPLDAR"
     gene            553..1020
                     /locus_tag="VE030_06185"
     CDS             553..1020
                     /locus_tag="VE030_06185"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HZF23039.1"
                     /translation="MAQFPNNSIAESLNHPRSNCALCAQAGGEVLWDDGFARVALIGD
                     ADHPAYCRVILNAHRKEMTDLAEAERERLMNIVFVVERVLRELLAPEKINLASFGNMV
                     PHLHWHIIPRFADDPHYPNPVFGARQRDGAHPLPAGFVRSMRQQLSGLLEKTP"
     gene            complement(1017..2351)
                     /gene="phoR"
                     /locus_tag="VE030_06190"
     CDS             complement(1017..2351)
                     /gene="phoR"
                     /locus_tag="VE030_06190"
                     /inference="COORDINATES: protein motif:HMM:NF008235.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HZF23040.1"
                     /translation="MWGRPLSTVVLIGILALVSWPILGSEGALLVLSALLLALLFHHL
                     FNLSALYRWLQNARPENAPVGSGAWEYVFSHLLRMLKRHKQSEVRLSEALSRFQLAGA
                     ALPDAVVILGEGDRIEWCNPKAEDYFGLDNERDRGQQITYILRQPQFVEHLSSGQVGE
                     SLVLRVSREQGEMILSVQLVPYGDRHKLVLGRDITRWERLETTRRDFVANVSHELRTP
                     LTVVGGFLETLADMKNPDAEMTQRSIMLMAQQTSRMTRLVEDLLTLSRLESTHNPLRE
                     EDVNVPDLVKALHQDAIALSAGRHKMRVRIELNDWLKGNTEELRSAFGNLISNAVRYT
                     PENGEVEIRWERHEGHSVFAVRDTGIGIEPQHIDRLTERFYRVDGSRSRETGGTGLGL
                     AIVKHVLNRHQARLEVQSNLGRGSTFSVIFPDTRRLPAKGLAATIQEPAISR"
     gene            complement(2433..3119)
                     /gene="phoB"
                     /locus_tag="VE030_06195"
     CDS             complement(2433..3119)
                     /gene="phoB"
                     /locus_tag="VE030_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HZF23041.1"
                     /translation="MAATILVVEDEPAIQELIAYNLKQAGHQPLRADNAEQAMNLVQS
                     ALPDLVLLDWMLPGQSGIDFARRLRTDKRTRTVPIIMLTARSDEQDKLMGLDAGADDY
                     ITKPFSPRELNARIKAVLRRRAPQMTDDVVQLGGLKLDPASHRISGNGEPVILGPTEF
                     RLLHFLMTHPERVHSRTQLLDQVWGDHVFVEERTVDVHIRRLRKALEPTRLDRLVQTV
                     RGTGYRFSAI"
     gene            3295..3717
                     /locus_tag="VE030_06200"
     CDS             3295..3717
                     /locus_tag="VE030_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HZF23042.1"
                     /translation="MAGLQPGAPRPTEIKLHQKSRVLEIAFDDGKSFRLECELLRVYS
                     PSAEVRGHGPGQEVLQIGKKDVEITAVEPVGNYAVRLVFSDGHDTGLYSWDYLYALGE
                     DKERLWKEYLDRMAKEGVSREAAPTTAQSQPKPSWKKL"
     gene            3929..4666
                     /gene="ubiE"
                     /locus_tag="VE030_06205"
     CDS             3929..4666
                     /gene="ubiE"
                     /locus_tag="VE030_06205"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HZF23043.1"
                     /translation="MDKKTTHFGYQEVDEEDKAKKVAGVFRSVAQRYDLMNDLMSAGM
                     HRLWKRFAVQMSGVREAGRVLDVAGGTGDLSALFAARVGMTGQVWLTDINGAMLVRGR
                     DRLLDRGRVVPVAQCDAERLPFPDDYFDCAVVGFGLRNMTHKESALAEMLRVLRAGGR
                     LLVLEFSQVWKPLKPLYDGYSFKVLPLLGKLVADDADSYRYLAESIRRHPGQEELKRM
                     MEQAGCARVEYYNLGAGVVALHRGHKI"
     gene            4672..5256
                     /locus_tag="VE030_06210"
     CDS             4672..5256
                     /locus_tag="VE030_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sterol-binding protein"
                     /protein_id="HZF23044.1"
                     /translation="MLAAAFVAFANHLLESAPWARDKLSLHAGKTALFDVFPVRLAFS
                     VAADGRLQPAPPEASPAVTIRLSHLTLIAVLADGEEAWRKAEVVGDTDFAAVISQVAS
                     NLRWDVEEDLSRVVGDIAAHRMAQAGRAAAAWPKQAAVSMAENAAEYLTEEKAVLVTP
                     LQAAEFMREVDELRDAVERMDKRIEHLSRPARGR"
     gene            5291..6814
                     /gene="ubiB"
                     /locus_tag="VE030_06215"
     CDS             5291..6814
                     /gene="ubiB"
                     /locus_tag="VE030_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HZF23045.1"
                     /translation="MHLLRLFKIVYVSLRFGLDEFFLGHARVRGLRALTAGLLFWRRL
                     GRPRGERLRLALEALGPIFVKFGQVLSTRRDLIPLDIADELAKLQDQVPPFAAAQVFQ
                     TLERAYGKPASAVFERFDAAAVASASVAQVHFAVLPGGREVAVKILRPGISRVIVEDI
                     ALLNVAAGLIERLWKDGRRLKPREVVGEFEKHLNDELDLLREAANASQLRRNFERSPL
                     LRVPEIFWDYCSSQVMVMQRMSGTPVSQVEKLRDQGIDIPTLARAGVEIFFTQVFRDG
                     FFHADMHPGNIFVAPDGRYIALDFGIMGTLTEVDKNYLAQNFLAFFRRDYRRVAQVHI
                     ESGWIPPQTRVDEFETAIRAVCEPIFDKPLKEISFGKVLLGLFQTSRRFNIEIQPQLV
                     LLQKTLLNIEGLGRDLDPDLDLWKTAKPYLERWMSEQVGWRGLLRNLRNEAPQWAVLL
                     PQLPRLVHRALSDRPANDLRAAMDALLQSQQRHTRLLTGILSVLLIILLLQIAAWLT"
     gene            7000..8499
                     /locus_tag="VE030_06220"
     CDS             7000..8499
                     /locus_tag="VE030_06220"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HZF23046.1"
                     /translation="MPNATKLQNSRPAPAPAKRLALAASIAALFCGFSASAFSAETET
                     LLDKLREKGVLTEEEYQEMRTEARTERRAKALKEASEEEKKTKKAESAASEITGKFKD
                     GFTFESGDGRHAFSVNGRIQGDYRAFEKSTSANTFDVRRAYLGVKGKFYDDITFNVTG
                     DFAQSSGNVGSGTTAIQLDEGYVNFGWIKWAQFRVGQFQMPFSLEKITSDLFSNFQER
                     NIGDVMLPDKLRGVMIHGSPTTGLYYGVGVTNGNGAKNTNDTNTVVDGKEAVGRLAAN
                     IAQMLDHKNSIYHFGVSYAETSMAPGAAFSGRTEARGITFFTPIAFTGSNVERKQAGL
                     ETALAYGPVKLQAESITTNYTGTLTGGTGYDKDIKAYYASINWLITGEKFADAYTNGV
                     FGRIKPNANFTTGGGTGAWEVGVRLSQWDAGDFVGAPGSTAAGGFDSAKFTSKAKATT
                     VGLKWITNPNTRILLNYIDTKFDTPVLISAAQGSVDREKALTLRAQFDF"
     gene            8671..9756
                     /locus_tag="VE030_06225"
     CDS             8671..9756
                     /locus_tag="VE030_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZF23047.1"
                     /translation="MALLEIGNVTRRFGDFTAVNDVSISIEAGEFFTLLGPSGCGKTT
                     LLRMIAGFDVPDDGSIVVDGQDMAGIPPEARNIHTVFQSYALFPHMNVEDNIAFPLRM
                     AKADPAQISRRVEETLEDVRLTGMNKRYPDELSGGQKQRVAVARALINRPKLLLLDEP
                     LAALDAKLKESVQLELIGLQKEIGVTFVYVTHDQGEALALSHRIAVMNHGRVEQVDEP
                     SKIYSFPKNRFVADFIGQCNLLEGEIKACKGERMTVHLDGIGEVSALNVEEDQPGLRG
                     VLALRPEKVAIGRDLPVGPDLNRFAGKVYDFLYLGDVTVYIVELAGGKRIEALLPNSA
                     AGRTKFFEAGDDVQVSWRYDAGHFLND"
     gene            9973..10869
                     /locus_tag="VE030_06230"
     CDS             9973..10869
                     /locus_tag="VE030_06230"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZF23048.1"
                     /translation="MTSGVKQNRLHKWLISGPPLAYLLVFFAIPVLIMVVASFRHPGD
                     FGGLAPLLYRDEAGAHLGLTAENYGRFFSDFLYTRLFIKSFAYAAITTLVCLVIAYPL
                     SMLIARSPKRHRDLLVLLVILPFWSNFLIRVYAWMIILGPQSVFVRVLNAFIGFFGFE
                     PVSLLFTPFAVIVGLVYVHLPFMVLPLYANLEKHDAALLDAAQDLGANAWQRFWRVTF
                     PLSMPGVYAGAALVFIPALGIFAIPDILGGTEGIMIGNVIKQQFLDTRDWPFGSVLSM
                     VLTVVAVGLAALAAWVARRSRV"
     gene            10866..11639
                     /locus_tag="VE030_06235"
     CDS             10866..11639
                     /locus_tag="VE030_06235"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZF23049.1"
                     /translation="MNRRSPWLWLASLFAYAFLYAPLVIVVAYSFNDSRLNAEWVGFT
                     FDWYQKLFHNEKMLTAAGNSLVIGLTASAVSTVLGTMAGYAMYRYKMRLLPVLVLAPI
                     AIPEILMGVSLLIFFVMLNITLGMVSIILSHIAFCVGFVAIVVRSRLAGMDESLTEAA
                     RDLGATPWQAFRLVTLPLIMPGVVAGALMAFTLSIDDFVITFFTAGVGSSTLPLQIYT
                     MVKIAATPEVNAVSTLLMLLTLALTLIASKLSPGALRSQ"
     gene            11760..12818
                     /locus_tag="VE030_06240"
     CDS             11760..12818
                     /locus_tag="VE030_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HZF23050.1"
                     /translation="MKKLLPLLLLLASLPAPAKDELHLYNWNDYIAPEAVKGFEQQCK
                     CKVVQDFYGDNEELVAKLAAGAKGYDVMIPTSNYVAGMAKAGWLQPLDHGKIPNFKNL
                     MPQYLNTEFDPGNKYSAPYAMSITLIGYNEQKMKQLGITVDSWAVIFDPRILQKIKGK
                     VTVLDSQGELMAAALKYHGYSVNDRDPDHWQQAKETILKAKPYWAAFMAQGYIKELST
                     GNIWVSHGYSNDIFQADNGAREAKQTFRIRHALPKEGAVLALDAMVIAKDAPRPDLAH
                     QFINFMLDGRNSAALTNITGSGNPNADAMKYIKPEIKNNKAVFPDAESAKRLEQQKDM
                     TSKQRQLLNRIWTEIKAK"
     gene            complement(12846..13823)
                     /locus_tag="VE030_06245"
     CDS             complement(12846..13823)
                     /locus_tag="VE030_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HZF23051.1"
                     /translation="MRFYDIFNGDADGICALHQLRLAEPRDAELVTGVKRDIELLARV
                     EPRRGDEIAVLDVSLESNRGHLIRMLETGVRCRYFDHHFAGAVPSHPLLDAHIDTSPS
                     VCTALIVDRYLHGEHRAWAVVAAFGDNLPEAALAAAADLHISAHELEVLRELGECLNY
                     NAYGESAEDLYFHPAELYRRLHDYPDPFAFAARASEFDVLRRAYAGDLEQARKLPVLQ
                     RGESCIAVFMPDEDWCRRVAGAFANDLSLRHPARAVALLVRRQGGYRVSLRAPQSAAQ
                     GIQHVARQFNSGGGRERAAGIQFLAEHDVERLLELLERTYAAKTPATGV"
     gene            13883..14992
                     /locus_tag="VE030_06250"
     CDS             13883..14992
                     /locus_tag="VE030_06250"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HZF23052.1"
                     /translation="MSRPFFSPATRYSRRSKLVRYLAVAYPLLVAYASLYPFSGWRAP
                     VEDATAFVLADWPFYVTFSDITLNVLAYLPLGLLLTLTFMGRTPRWAAAMLGVLTGTL
                     FSFLIELAQGYLPSRIASNVDLLTNAGGTVLGAAAAYAFGERWLLSGELFRLRGRYFL
                     PGATTDYGFVLLGLWLFTQLNADIWLFGNGDLRHLVPGEVGVSYSAESYRYLEAGVAA
                     LNFAGVAFLLTAIARSFLGAAVSVIALTAAALALKTIASSALFIPGNPSLWLTPGSLL
                     GLAIGFAAWLLLAGAPRSVLIRAAVVCLALGVVVVNLAPENPYLVAALKVWRHGHYVS
                     FNGMTRLLSALWPFLTLGYLILLAREAEGAPAARI"
     gene            15007..15339
                     /locus_tag="VE030_06255"
     CDS             15007..15339
                     /locus_tag="VE030_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HZF23053.1"
                     /translation="MITTPDSLSELRERLRQFAAERDWEQFHVPKNLAMSVAIEAAEI
                     MEHFQWLTPDESAALPEPQRSAVAHEIADVLLYLVRLADVLDIDLVEAALDKMAINAH
                     KYPVVRGI"
     gene            15359..>15577
                     /locus_tag="VE030_06260"
     CDS             15359..>15577
                     /locus_tag="VE030_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015877126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HZF23054.1"
                     /translation="MFPVSHYKYHVFFCCNQREPGAACCNNHHAQEMRDYAKARVKSL
                     QLAGPGKVRVNQAGCMDRCDEGPVIAIYP"
BASE COUNT         2900 a         4780 c         4852 g         3045 t
ORIGIN      
        1 gttcatctgc cttatgcgct ggcgaggaag tatccgcgcg cgggatacga atggatctgg
       61 caatacgttt ttccctcgaa gaaccgctcg gcagatcccg acgacggcgt aatccggcgg
      121 catcatctcg atgagtcggt cctccagcgt gcggtcaaga aggccgttcg catcgtcggt
      181 atatcaaaac tggttcactg ccatacattc cgccacagtc ttgccacgca cgtattgcaa
      241 agtgggtatg acatccgtac ggtgcaggaa ctattgggtc attcggacgt atcgacaacc
      301 atgatctaca cgcacgtact gaatcgtggg ggccgaggag tgataagccc gctcgatgcg
      361 cgttgattta agtgattggg tgattgggtg attgggtgat tgagtgattg agtaattgag
      421 tgatcaggtg atcaggtgac caattcatgc ggcatcgcgc accgcccgtt gtttgatggc
      481 tcaacccgcc aatcactcag tcactgaatc actcaatcac cctagcagca actgcgcgcc
      541 gcccctcgtt cgatggctca atttcccaat aactcaatcg ctgaatcact caatcaccct
      601 cgcagcaact gcgcgttgtg cgcgcaggcc ggcggagagg tcctctggga cgacggtttc
      661 gcccgggtgg cgctgatcgg cgatgccgat catcccgcct attgccgggt gatcttgaac
      721 gcccacagga aagagatgac cgacctcgcc gaagccgagc gcgagcgtct gatgaacatt
      781 gtgttcgtcg tggagcgcgt gctgcgcgag ctgctcgctc cggaaaagat caacctggcc
      841 agcttcggca acatggtgcc gcacctgcac tggcacatca ttccgcgttt tgccgatgat
      901 ccgcattatc ccaacccggt attcggagcc aggcaacgtg acggtgccca tccgctgccg
      961 gcgggattcg tccgctcgat gcgacagcag ttgtccgggt tactggagaa aaccccctag
     1021 cgggaaatcg cgggttcctg gatcgtagcc gccagccctt tggcgggcag gcgccgggtg
     1081 tccggaaaaa tgaccgagaa ggtgctgccg cgtcccaggt tgctctggac ctcgagccgc
     1141 gcctggtggc gattgagcac gtgcttgacg atagccaggc ccagtccggt gccgccggtt
     1201 tcgcgcgagc gactgccgtc cacccggtag aagcgctcgg tcaggcggtc gatgtgctgc
     1261 ggctcgatgc cgatgccggt gtcccgcacc gcgaacaccg aatggccttc atgtctttcc
     1321 cagcgtattt ccacctcacc gttttccggc gtatagcgca ccgcattgct gatcaggttg
     1381 ccgaaggcgc tgcgcagctc ctcggtgttt cccttcagcc agtcgttcaa ttcgatcctg
     1441 acgcgcatct tgtgccggcc ggcggaaagc gcgatcgcgt cctggtgcag cgccttgacg
     1501 aggtccggaa cgttgacgtc ctcctcgcgc agcggattgt gcgtgctctc cagccgcgac
     1561 aacgtcaaca agtcttccac cagccgggtc atgcggctgg tttgctgcgc catgagcatg
     1621 atcgagcgct gcgtcatttc cgcatccgga tttttcatgt cggccagggt ttccagaaag
     1681 ccgccgacca cggtcagcgg cgttcgcaac tcgtgcgaca cgttggcgac gaaatcgcgc
     1741 cgggtcgtct ccagccgttc ccagcgggtg atgtcgcgcc caagaacgag tttgtgccgg
     1801 tcgccgtagg gaaccagttg caccgacaga atcatctcgc cctgctcgcg cgaaacccgc
     1861 agtaccagcg attcaccgac ctggccggag gacaggtgtt cgacgaactg cggctggcgc
     1921 aggatgtagg tgatctgctg gccgcggtcg cgttcattgt cgagaccgaa gtagtcctcc
     1981 gccttggggt tgcaccactc gatgcggtcg ccttcgccca ggattaccac cgcatccgga
     2041 agcgccgcac ccgcgagttg aaagcgcgac agcgcctcgc tcagccgcac ttcgctttgc
     2101 ttgtggcgct tgagcatcct gagcaggtgc gagaacacgt actcccaggc cccgctgcct
     2161 accggcgcgt tctccgggcg ggcgttctgc agccagcggt aaagcgccga aaggttgaaa
     2221 aggtgatgga aaagcagggc caggagaagc gcggagagga ccagcaaggc accctcgctg
     2281 ccgagaatcg gccaggagac cagcgccaga atgccgatca ggacgacggt ggagaggggt
     2341 ctgccccaca tggacttcaa gcgttccgtc ctctgggtta gccagggcgg attatcccag
     2401 aattcaccgg agcggacccc cggagacgcc ggtcagattg ccgagaagcg gtagccggtg
     2461 ccgcgcacgg tttgcaccag gcggtcgagc cgggtgggtt cgagcgcctt gcgcagtcgc
     2521 cggatgtgca cgtccacggt gcgctcttcc acgaagacat ggtcgcccca tacctggtcc
     2581 agcaactggg tacgcgaatg cacgcgctcc gggtgcgtca tcagaaaatg cagcaagcgg
     2641 aattcggtgg ggcccagtat caccggttcg ccgttgcccg agatgcggtg gctggccgga
     2701 tcgagcttca gtccccccag ttgcactaca tcgtcggtca tctgcggcgc acgtcgccgc
     2761 aacactgcct tgatgcgcgc gttcagctcc cgcggcgaga acggcttggt gatgtagtcg
     2821 tccgcaccgg cgtccaggcc catcagcttg tcctgctcgt cggagcgcgc ggtgagcatg
     2881 atgatcggca cggtccgggt tcgcttgtcg gttcgcagcc tgcgggcaaa atcgatcccg
     2941 gactgtccgg gcagcatcca gtccagcagt accagatcag gcagggcgct ctggaccagg
     3001 ttcatcgcct gctcggcgtt gtcggcgcgc aatggctggt ggccggcctg tttcaggttg
     3061 tacgcgatca actcctgaat tgccggttcg tcttcgacca cgaggatggt ggctgccatg
     3121 ggctatacca gtcggatttg agcgtttgca atactatgaa gcaaatgtta caaccacatg
     3181 accatttttg acgggtaata acaggtcgct cacatgaccg aggccccgtc cgcggctatg
     3241 atgcagtccc gcgcctgcat tcctaggcat tgaattctac gcacgaaata tcccgtggcc
     3301 ggacttcaac ccggggcgcc ccgccccact gagatcaagc tccaccagaa gtcgcgcgtc
     3361 ctcgaaatcg cgttcgacga cggcaagtcg ttccgactgg agtgcgagct gttgcgtgtt
     3421 tactcgccat ctgcggaggt gcgcggccac gggccgggcc aggaggtgct gcaaatcggc
     3481 aagaaggacg tcgagatcac ggcggtcgag ccggtgggca attacgcggt gcggctggtg
     3541 ttcagcgacg gacacgacac cgggttgtac tcgtgggact atctttacgc actcggcgaa
     3601 gacaaggaaa ggttgtggaa ggaatatctg gaccggatgg cgaaggaagg cgtcagccgc
     3661 gaggccgcgc cgaccaccgc ccagtcacag cccaaaccca gctggaaaaa gctctgacct
     3721 accctgacaa gaacaactct gaacgcagag gacgcagggg acgcgaagga aaaatctgaa
     3781 atgatgacaa gcttgggcag gccgcgagca atacggagaa gttgtttgct tactgcgcac
     3841 attctttgca tccttacgct gcagttcccc ttttttgttg gggtaccttt gcgtcctccg
     3901 cgtcctctgc ggtgagggtt tgaatttcat ggacaagaag accacgcact tcggatatca
     3961 ggaagtcgac gaggaagaca aggcgaagaa agtcgctggg gtgttccgct cggtggcgca
     4021 gcgttacgac ctgatgaacg atctgatgtc ggcgggaatg caccggctgt ggaagcgctt
     4081 cgcggtgcag atgagcgggg tgagggaagc cggccgggtg ctggacgtgg ccggcggaac
     4141 cggggatctg tccgcgctgt tcgcggcgcg ggtcggcatg accgggcagg tgtggctgac
     4201 cgatatcaac ggcgcgatgc tcgtgcgcgg gcgcgaccgg ctgctcgacc gcggccgcgt
     4261 cgtgccagtc gcgcagtgcg acgccgaacg cctgccgttt ccggacgatt atttcgattg
     4321 cgcggtggtg ggcttcgggc tgcgcaacat gacgcacaag gaaagcgcgc tggcggaaat
     4381 gctgcgcgtg ctgcgcgccg gcggacgcct gctggtgctg gagttctcgc aggtgtggaa
     4441 gccgctcaag ccgctgtacg acggctactc gttcaaggtg ctgccgctgc tgggcaagct
     4501 tgtagctgat gatgcggaca gctatcgcta cctcgccgag tcgattcgcc gccatcccgg
     4561 gcaggaagaa ctgaagcgga tgatggaaca ggccggctgc gcacgggtcg agtactacaa
     4621 cctcggcgcc ggggtggtgg cactgcaccg cggccacaag atctgaagcc gatgcttgcc
     4681 gccgcgtttg tagcgttcgc caatcacctg ctcgagtccg cgccctgggc gcgcgacaaa
     4741 ctgagcttac acgccggcaa gactgcgctg ttcgacgtgt ttccggtccg gctggcgttc
     4801 tcggtggcgg cggacgggcg gttgcagccg gcgccgccgg aagcctcgcc ggcggtgacg
     4861 atccgcctct cgcacctgac cctgatcgcg gtgttggctg acggcgagga ggcctggcgc
     4921 aaggccgaag tcgtcggcga taccgatttt gccgcggtca tttcccaggt cgcgtccaac
     4981 ctgcgctggg atgttgaaga ggacctgagc cgggtggtcg gcgatatcgc ggcgcatcgc
     5041 atggcgcagg ccggacgggc ggccgcggcg tggccaaagc aggccgccgt gagcatggcc
     5101 gagaacgccg cggaatatct caccgaggag aaggcggtac tggttacgcc gttacaagcc
     5161 gccgagttca tgcgcgaggt cgacgaactg cgcgatgccg tggagcgcat ggacaagcgc
     5221 atcgagcacc tgtcgcggcc ggcgcgcggc agatagaaaa acaaaggaag cgcggcgcaa
     5281 tcgccctgtc atgcacctgc tgcggctgtt caagatcgtg tacgtctcgc tgcgcttcgg
     5341 gctggacgaa ttcttccttg gccatgcccg ggtgcgcggg ttgcgtgcgc tgaccgcggg
     5401 gttgctgttc tggcgccgcc tcgggcggcc gcgcggagaa cgcctgcgcc tcgcgctgga
     5461 agcactcggg ccgatattcg tgaagttcgg gcaggtgctg tccacccgcc gcgaccttat
     5521 cccgctcgac attgccgacg aactggcgaa actccaggac caggtcccgc cgttcgcggc
     5581 cgcgcaggtg ttccagaccc tggagcgcgc ctacggcaag cctgcctcgg cggtgttcga
     5641 gcgcttcgat gcggcggcgg tcgccagcgc ctcggtggca caggtgcatt tcgcggtcct
     5701 gcccggcggg cgggaagttg ctgtgaagat cctcaggccg gggattagtc gcgtcatcgt
     5761 cgaagacatt gcgctgctca atgtggccgc cggcttgata gagcgcctgt ggaaggatgg
     5821 ccgccgcctc aagccacggg aagtggtcgg cgagttcgag aagcacctca atgacgaact
     5881 cgacttgctg cgcgaggcgg cgaacgccag ccaactacgc cgcaacttcg agcgttctcc
     5941 cctcctgcgg gtgccggaga ttttctggga ctattgcagc agccaggtga tggtgatgca
     6001 gcgcatgagc ggcacgccgg taagccaggt cgaaaaactg cgggatcagg gtatcgacat
     6061 cccgacactt gcgcgcgccg gtgtggagat ctttttcacc caggtgttcc gcgacggctt
     6121 cttccacgcc gacatgcacc ccggcaacat cttcgtcgcc ccggacgggc gctatatcgc
     6181 gctggatttc ggcatcatgg gcacgctcac cgaggtggac aagaattacc tggcgcagaa
     6241 tttcctcgcc tttttccggc gtgactaccg gcgcgtggcg caggtccaca tcgaatccgg
     6301 ctggatcccg ccgcagaccc gcgtggacga attcgaaacc gcgattcgcg cagtttgcga
     6361 gcccatcttc gacaagcccc tgaaggagat ttccttcggc aaagtgctgc tgggcctgtt
     6421 ccagacttcg cgccgtttca acatcgagat ccagccgcaa ctggtgctgc tgcaaaagac
     6481 cctgctcaac atcgaaggcc tgggcaggga cctcgatccc gacctggatc tctggaaaac
     6541 ggccaaaccc tatctcgagc gctggatgtc ggagcaggtc ggctggcgcg gcctcttgcg
     6601 caacctccgc aacgaggcgc cgcagtgggc ggtactcctg ccgcagctgc cgcgcctggt
     6661 gcatcgcgcg ctttcggatc gtcctgccaa cgacttgcgc gcggcaatgg atgcgttgct
     6721 gcaaagtcag cagcgccata cccgccttct gaccggaatc ctctccgtcc tactgattat
     6781 tctgctgttg cagatcgcag cctggctcac ctaagtcgcg tccgcggcaa tgttcctgtc
     6841 acgacgggga catgtttcgt taatcagatt gtcatatttg gcctccagaa tccggtcccg
     6901 ttggggcgcc ggcttcgtgc ctgcgtgccg cctcccgtga aatccgggca ttcgctgccg
     6961 cgcaacgggt gcgcaagttt tcaccaccag gagacatcga tgccaaacgc aaccaaattg
     7021 caaaattcca ggccggcacc tgccccggcg aagcgccttg cactcgcagc ctcgatcgcc
     7081 gcgctgttct gtggcttttc cgcctcggcg ttttccgcgg agaccgagac tctgctcgac
     7141 aagcttcgcg agaaaggcgt cctgaccgag gaggaatacc aggaaatgcg caccgaggcg
     7201 cgcaccgagc ggcgcgcgaa ggcgctgaaa gaagccagcg aagaggagaa gaagaccaag
     7261 aaagccgaat ctgccgcctc ggaaattacc ggcaagttca aggacggttt caccttcgaa
     7321 tccggagacg gtcggcacgc gttttcggtg aatggccgga tccagggcga ctaccgcgcg
     7381 ttcgaaaaaa gtaccagcgc caacacgttt gacgtccgcc gcgcatatct cggggtcaaa
     7441 ggcaagttct acgacgacat taccttcaac gtgaccggcg atttcgccca gtccagcggc
     7501 aatgtcgggt ccggcaccac tgcaattcag ttggatgaag gttacgtgaa ctttggctgg
     7561 atcaagtggg cgcagttcag ggtcggccag tttcaaatgc ccttcagcct ggaaaagatc
     7621 accagcgatc tgttcagcaa tttccaggag cgcaatatcg gtgacgtgat gttgcccgac
     7681 aaactgcgtg gcgtgatgat ccacggcagc ccgaccaccg gcctctacta cggggttggc
     7741 gtgacgaacg gcaacggggc aaagaacact aacgacacca ataccgtggt tgatggaaaa
     7801 gaggcggtgg gccgccttgc cgcgaacatc gcgcagatgc ttgatcataa gaattccatt
     7861 tatcatttcg gcgtatccta cgcggagact tccatggcgc ctggtgcggc cttctccggc
     7921 cggaccgaag cgcgcgggat cacttttttc acgccgatcg cattcaccgg ctccaacgtc
     7981 gaacgcaaac aggcgggatt ggagacggcg ctggcctatg gcccggtgaa gctccaggcg
     8041 gaatccatta ctacgaatta cacgggcact ctcaccggcg gaaccggtta cgacaaggac
     8101 atcaaggcct attacgccag catcaattgg ttgatcaccg gggagaaatt cgcggacgcc
     8161 tataccaacg gcgtgtttgg gcgcatcaag ccgaacgcca atttcactac tggcggcgga
     8221 accggcgcct gggaagtcgg agtccgtctc agccagtggg acgccggtga tttcgtcgga
     8281 gccccgggca gcacagcggc gggaggattc gactcggcca agttcaccag caaggccaag
     8341 gcgacaaccg tcggcctgaa gtggatcacc aacccgaata cccgcattct gctcaactac
     8401 atcgatacca aattcgacac gccggttctg atctctgcgg cgcaaggttc agtggatcgt
     8461 gaaaaagcgc tgactctgcg cgcgcaattc gacttctgag agactgagac tccttcaagc
     8521 ttgagccggt aaaccctgct gccgcgggtt cctcaaggga cccgcggttt tttttgccgt
     8581 tgccgcgaat acccggccgt ttccccgcat cattcatggg gttggtagaa ttgccgcttt
     8641 gcaaaatcag ggcgggacac agggcggggc atggcgctac tcgagatcgg caacgtcacc
     8701 cgtcgtttcg gcgatttcac cgccgtcaac gatgtcagca tcagcatcga agccggagag
     8761 ttcttcaccc tgttgggtcc gtccggctgc ggcaagacca cgctgttgag gatgatcgcc
     8821 ggattcgacg tgcccgatga cggaagtatc gtcgtcgacg gccaggatat ggccggcatt
     8881 ccaccggaag cgcgcaatat ccatacggtg ttccagagct acgcgctgtt cccgcacatg
     8941 aatgtcgagg acaacatcgc cttcccgttg cggatggcga aggcggaccc ggcgcaaatc
     9001 agccggcggg tggaagagac gctggaagac gtgcgcctca ccggcatgaa caagcgctat
     9061 cccgatgaac tttccggcgg ccagaagcag cgcgtggcgg tggcgcgggc gcttatcaac
     9121 cggcccaagc tgttgctgct cgacgaaccg ctggcggcgc tggacgcaaa gctcaaggaa
     9181 agcgtacaac tcgagctcat cggcctgcag aaggaaatcg gcgtcacttt cgtctacgtg
     9241 acccacgatc agggcgaagc actggcactg tcgcaccgga tcgcggtgat gaaccacggg
     9301 cgcgtcgaac aggtcgacga gccgtcgaag atctacagtt ttccgaagaa ccgcttcgtc
     9361 gccgacttca tcggccagtg caacttgctc gagggcgaaa tcaaggcctg taagggcgag
     9421 cgcatgacgg tgcacctcga cggcatcggc gaggtttccg cgctcaatgt ggaggaggat
     9481 caaccggggt tacggggcgt gctggcgttg cgtccagaga aagtggcgat cggccgcgat
     9541 cttcccgtcg ggccggacct gaaccgtttc gcgggcaagg tctacgactt cctttatctc
     9601 ggcgacgtca ccgtgtacat cgtcgagctt gccggcggca agcgcatcga ggcgctgctg
     9661 cccaactccg ccgcggggcg caccaagttc ttcgaggccg gcgacgacgt tcaggtgagc
     9721 tggcgctacg acgccggcca ttttctgaat gactgacatc gaaaagctca ccacgaaggc
     9781 acgaagacac gaggaagaac aggaatcctt aaacgaattg gggatagaag cgcggtgact
     9841 gttcgtgacc ctctcccgcc agcttcgact ttgatggaat atccgccaat tggagctttt
     9901 tacacacatt tgttttttcg ctcttcttcg tgtcttcgtg ccttcgtggt gagatttgcc
     9961 gtttgacgga acatgacatc cggagttaaa cagaaccgcc tgcacaagtg gctgatcagc
    10021 ggcccgccgc tcgcctatct gctggtgttc ttcgctattc ccgtgctgat catggtggtg
    10081 gcgtcgtttc gccacccggg cgatttcggc ggtctggcgc cgctgctgta tcgcgacgaa
    10141 gccggcgctc acctcggcct taccgcagag aactatggcc gcttcttctc cgatttcctc
    10201 tatacccggc ttttcatcaa gtcgtttgcc tacgcggcga tcacgacgct ggtatgcctg
    10261 gtcatcgcct atccgctgtc gatgctgatc gcccgcagcc cgaaaagaca ccgcgatctg
    10321 ctggtgctgc tggtgatcct gccgttctgg tcgaatttcc tgatccgcgt ctacgcgtgg
    10381 atgatcatcc tcggaccgca gtcggtgttc gtgcgggtgc tgaacgcctt catcggcttc
    10441 ttcggcttcg agccggtgtc gttgctgttt acgcccttcg cggtcatcgt cggcctggtc
    10501 tacgtgcatc tgccgttcat ggtgctgccg ctgtacgcga atctggagaa gcacgacgcg
    10561 gctctgctcg acgcggcgca ggatctgggc gcgaatgcct ggcagcgctt ctggcgggtg
    10621 actttcccgc tgtcgatgcc cggcgtgtac gccggcgcgg cgctggtgtt catccccgcg
    10681 ctcgggatct tcgccatccc cgatatcctc ggcggcaccg agggcatcat gatcggcaac
    10741 gtgatcaagc agcagttcct cgacacccgc gactggccgt tcgggagcgt gctgtcgatg
    10801 gtgctcacgg tagtggccgt ggggctggcc gcgctcgcag cctgggtggc gcggagatcg
    10861 cgggtatgaa ccgccgttcg ccgtggctct ggcttgccag cctcttcgcc tacgcctttc
    10921 tgtatgcacc gctggtaatc gtggtcgcct attccttcaa cgattcgcgt ctgaacgccg
    10981 aatgggtcgg attcaccttc gactggtacc agaagctgtt ccacaacgag aagatgctca
    11041 cggcggcggg gaactcgctc gtgatcgggt tgaccgccag cgcggtctcc accgtcctcg
    11101 gcaccatggc cgggtatgcg atgtaccgct acaaaatgcg gctgctgccg gtgctggtgc
    11161 tggctccgat agcaattccc gaaatcctca tgggcgtgtc gttgctgatt ttcttcgtca
    11221 tgctcaacat cacgctgggc atggtgtcga tcatcctgtc ccacatcgcc ttctgcgtcg
    11281 gcttcgtcgc catcgtggtg cgctcgcggc ttgccggcat ggacgagagc ctgaccgaag
    11341 cggcccgcga tctgggcgcc acgccgtggc aggcctttcg ccttgtgacc ctgccgctga
    11401 tcatgccggg ggtggtcgcc ggcgcgctga tggcgttcac gttgtcgatc gacgacttcg
    11461 tcatcacctt cttcactgcg ggggtcggct cctccacgct gccgttgcag atctacacca
    11521 tggtaaagat cgcggcgacg ccggaagtca acgccgtgtc aactctgctg atgctgttga
    11581 cgttggcgct gacactgatt gcctcgaagt tgtcccccgg cgccctgcgc agccagtgag
    11641 gatgcacgca atcgccgtca tctcccggac ttactgacca ttccctctgg actgcgacgc
    11701 tcgtatttca tactctttgc gtttcgagga cggatatcgc ctgactccgg agagcgaaca
    11761 tgaagaagct gctacccctg ctgctgctgc tcgcaagcct tccggcgccg gcgaaggacg
    11821 agttgcacct ctacaactgg aatgactaca ttgcgcccga agcggtcaag ggcttcgagc
    11881 agcaatgcaa gtgcaaggtg gtgcaggact tctatggtga caacgaggag ctggtcgcca
    11941 agctcgccgc cggtgccaag ggatacgacg tgatgatccc gacctccaat tacgtggccg
    12001 gcatggccaa ggccggatgg ctgcagccgc tcgaccacgg caagatcccg aatttcaaga
    12061 atctgatgcc ccagtacctg aacaccgaat tcgaccccgg taacaaatac tccgccccct
    12121 atgcgatgtc gatcacgctg atcggctaca acgagcagaa gatgaagcaa ctgggtatca
    12181 ccgtagactc ctgggcagtg atcttcgatc ccaggatcct gcaaaagatc aagggcaagg
    12241 tgacggtgct cgacagccag ggcgaattga tggcggcggc gctcaagtat cacggctatt
    12301 cggtcaacga ccgcgaccct gaccattggc aacaggcaaa ggaaaccatt ctcaaggcca
    12361 agccctattg ggcggccttc atggcgcagg gctacatcaa ggaactctcc accggaaata
    12421 tctgggtatc ccacggttac tcgaacgaca tcttccaggc ggacaacgga gcccgggaag
    12481 ccaagcagac attccgcatt cgtcatgccc tgcccaagga aggcgcagtg ctggcgctgg
    12541 atgcgatggt cattgccaag gatgccccgc ggccggacct cgcgcaccag ttcatcaatt
    12601 tcatgctcga cggcaggaac tccgccgccc tcaccaacat taccggctcg ggcaatccga
    12661 acgccgacgc gatgaaatac atcaagccgg aaatcaagaa caacaaggcg gtgttcccgg
    12721 acgcggaatc agccaaacgc ctggagcagc agaaggatat gaccagcaag cagcggcagc
    12781 tgctgaaccg gatctggacc gaaatcaagg cgaaatagca cgcgatggcg ccgccgcaag
    12841 ttcggctaaa cgccggtggc gggggtcttc gccgcgtagg tccgctcgag caattcgagc
    12901 agccgctcga cgtcgtgctc cgccaggaac tggatgccgg cagcacgttc ccggccgccg
    12961 ccggagttga attgacgggc gacatgctgg attccctgcg ctgcgctttg cggggcacgc
    13021 agacttaccc gatatccgcc ttgccggcgc accagcaacg cgaccgcgcg tgccggatga
    13081 cgcagtgata ggtcgttggc gaatgcgccg gcgacgcgcc ggcaccagtc ttcgtccggc
    13141 atgaacaccg caatgcaact ctcgcctcgt tgcaacaccg gtagcttgcg cgcctgctca
    13201 aggtcgccgg cgtaggcgcg gcgcagtacg tcgaattcgg acgcgcgcgc ggcaaatgcg
    13261 aacgggtcgg gatagtcgtg caagcggcgg taaagttcag cgggatgaaa atacaggtcc
    13321 tccgccgatt ctccgtaggc attgtagttc agacattccc ccagttcgcg cagcacctcg
    13381 agttcgtgcg ccgaaatgtg caggtcggcg gctgcggcaa gcgcggcttc cggcaggttg
    13441 tccccgaacg cagcgacgac cgcccacgcc cggtgctccc cgtgcagata acgatcgacg
    13501 atcagggcgg tgcagaccga aggtgaggtg tcgatatgcg cgtcgagaag cgggtggctg
    13561 ggaaccgcac cggcgaagtg atgatcgaaa taccggcaac gtaccccggt ctccagcatg
    13621 cgaatcagat ggccacggtt agattccagc gaaacgtcga gcaccgcaat ttcatctccg
    13681 cgccggggct ctacgcgggc gagcaactcg atatcgcgct tgaccccggt gacgagctcc
    13741 gcgtcgcgcg gctcggcaag gcgcaattga tgcagagcgc agatgccgtc ggcgtcaccg
    13801 ttgaagatgt catagaatct caatcagagg ttccaatggc tgcggtatcg tttgattgcg
    13861 cccggcgcgc tcccacaagt acatgagccg tccgtttttc tccccggcaa cgcgttactc
    13921 gcgcagatcc aagctggtgc gttacctcgc ggtagcttac ccgctgctgg tggcgtatgc
    13981 cagcctctac ccgttctccg gctggcgtgc gccggtcgaa gacgccaccg ctttcgttct
    14041 cgccgactgg cccttctacg tgaccttttc cgatatcacg ctcaacgtac tcgcctatct
    14101 acccctcgga ctgctgctca ccctgacgtt catggggcgc acgccgcgct gggcggcggc
    14161 gatgctcgga gtgttgaccg ggacgctgtt cagttttctc atcgagctgg cgcagggcta
    14221 tttaccgtcg cgcattgctt ccaacgtcga tctgctcacc aacgctggcg ggacggtgct
    14281 cggtgccgcc gcagcctacg cgttcgggga acgctggctg ctctccggcg aactgttccg
    14341 gctgcgtggc cgttactttc tgcccggcgc gacgaccgac tacggcttcg tgcttctggg
    14401 gctgtggctg tttacccagc tcaacgcgga catctggctg ttcggcaatg gcgatctgcg
    14461 ccacctggtg ccgggtgagg tcggcgtgag ttattcagcc gagtcctacc gttacctgga
    14521 agctggcgtt gccgcgctca attttgccgg tgtggctttt ctgctcaccg caatcgcgcg
    14581 atcgttcctc ggtgccgcgg tctcggtcat cgcgctgacg gcagcggccc tggcactgaa
    14641 aacgatcgct tcctcggcgt tgttcattcc cggcaatcct tcgctgtggc tgacgccggg
    14701 gtcgctgctg gggctcgcta tcggcttcgc ggcgtggctg ctgctggctg gcgctccccg
    14761 gagtgttctc atccgggctg cggtggtctg ccttgcactc ggagtggtcg ttgtgaacct
    14821 cgcacccgag aatccctatc tggtcgcggc attgaaagtg tggcggcacg gacattacgt
    14881 cagcttcaac ggcatgacac ggctgctctc ggcattgtgg ccctttctca cgctcggtta
    14941 cctgattctt cttgcgcgcg aggccgaagg cgcgcccgct gcccggatct agggaaggca
    15001 aagacgatga ttacaactcc cgattctctg agtgaattgc gcgaacggtt gcggcagttc
    15061 gccgccgagc gcgactggga gcagttccat gttccgaaga acctggcgat gtcggtggcg
    15121 atcgaggccg ccgaaatcat ggaacacttc cagtggctga ccccggacga gagcgctgcg
    15181 ctgcccgagc cgcagcgctc cgccgtggcg cacgagatcg ccgacgttct gctttacctg
    15241 gtgcggctcg cggacgtgct ggacatcgat ctggtggagg ccgcgctgga caagatggcg
    15301 atcaacgccc acaagtatcc ggtcgtgcgc ggcatataat atgcggccat agcgaaggat
    15361 ttttccggtg agccattata agtatcacgt gttcttttgc tgcaaccagc gcgaaccagg
    15421 cgccgcgtgc tgcaacaacc atcacgcgca ggaaatgcgc gattacgcca aggcccgcgt
    15481 caagtcgctg cagctcgcag gcccgggcaa ggtgcgggtg aaccaggcgg gctgcatgga
    15541 ccgctgcgac gagggcccgg tgatcgcgat ttatcct
//
LOCUS       DASQOP010000151         4913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2480502, whole genome shotgun sequence.
ACCESSION   DASQOP010000151 DASQOP010000000
VERSION     DASQOP010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4913
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2480502"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1388)
                     /locus_tag="VE030_06265"
     CDS             complement(<1..1388)
                     /locus_tag="VE030_06265"
                     /inference="COORDINATES: protein motif:HMM:NF027664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HZF23055.1"
                     /translation="MRKLAVVFLLIFAAGCAAHTAMIEGRKLLEQGRTEEAIRRMERG
                     VKANPDDTELRNYYARNRDLYVNQLLYEGEKARMLGRSEGAIAAFQRVLELSAGNVRA
                     QAGLEAARAEQSQRAAVAQAQAMFDEGKPEEALELLRKVLGSNPSQKDARALQRRIEE
                     SRARSVVAAPRLKPEFRKPVTLEFRDAPLKSIFEVLSRTSGINFVFDRDVRPDLRATI
                     YVRDTSIEDAVNFLLVTNQLEKKVLNENTILLYPNLPNKTRDYQELVVKSFYLGNADV
                     KQTLNLIKTVVKTRDVFVDERLNLLVMRDTPDAIRLAEKLIAAQDLAEPEVILELEVL
                     EVTRSKMQNLGLQYPTQISAGVEGAAGKPGQITLTEWLNRSSGLVNLSITDPAFVLNL
                     KRQDGDTNLLANPRIRVKNREKAKVHIGDRLPVITTTSTANVGVSESVQYLDVGLKLD
                     VEPNVYLDDQVA"
     gene            complement(1385..1939)
                     /locus_tag="VE030_06270"
     CDS             complement(1385..1939)
                     /locus_tag="VE030_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23056.1"
                     /translation="MKLHTRQRWLVVAGLLTATLAAAAWIRDSAVPSGTEVVAASDSS
                     AAPAPRPSAAGTDGEAPAINLEKLKSRELGRAIRDPFTTGAPRAAKSKPAAAAPPAQV
                     VVAPAPSPPPLSAPPLPFTYMGKLVQGGDLAVFLVQGERNLIVHEGDTIDSLYKVERI
                     AEGGITLLFLPLNQRQTIIIGEPQ"
     gene            complement(1936..2493)
                     /locus_tag="VE030_06275"
     CDS             complement(1936..2493)
                     /locus_tag="VE030_06275"
                     /inference="COORDINATES: protein motif:HMM:NF022201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspMb/PilO family protein"
                     /protein_id="HZF23057.1"
                     /translation="MDAALKRIRWRLQRALHAMGWAAIGGVALAILAAGFVASNLMPL
                     QADVTALRDRVQRLELRAGNQARIVEVGRPDAGMTAFYEQLPAAEQAPEVVRRLHSYA
                     RDAGLVLERGEYRPLPDPSGKLMRYQVVVPVKGSYPQVKRFLTQAMHDTPGLALDAIS
                     MQRGQGGSPALEVQLRFTVFMRSAA"
     gene            complement(2478..3002)
                     /locus_tag="VE030_06280"
     CDS             complement(2478..3002)
                     /locus_tag="VE030_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23058.1"
                     /translation="MRAVQLDFLQAARRSSGGGIVLLAAAVVAAFVVLSFHADLKSEA
                     RHLESQASKLERRARGLAPAGPRVDESLKQEIRRVNEMIDRLALPWDRLFRSVEAAAT
                     DRVALLGIVPDAKSGIVQISAEASDAETMFDYVRRLEQQSELSQVYLLQHERERQNAV
                     RPIRFLVSASWMPR"
     gene            complement(3005..3817)
                     /locus_tag="VE030_06285"
     CDS             complement(3005..3817)
                     /locus_tag="VE030_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23059.1"
                     /translation="MSLSWRERVRIALSPHQVAMVRFSRGLGPRVVDRKTMPCAEAKG
                     HENWAGAVEVLRDLLPHPNVDKGDATLILSSHFVRYVVLPWSSELVTEAEELEFARTR
                     FIQVFGQSARDWTIAMSPAPAGASRLCAAVDQALIGAATGAVAASGLRLQSVQPALMA
                     QFNEWRRRIGADGWLVIAEQGRLLVAWLSAGQWRSVRVRPLHDASVSLAQVLEQEQLL
                     LSAGSANKVFLAKLGNVAVETDGLKVERLALPAPLARAAGANAGLALAMCGL"
     gene            complement(3798..>4913)
                     /locus_tag="VE030_06290"
     CDS             complement(3798..>4913)
                     /locus_tag="VE030_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HZF23060.1"
                     /translation="IHLESAGNRLAIKYRIDGVLSQVGVAQGADMAEQIVSRVKVMAE
                     LDIAERRIPQDGRFKVRVKGREVDFRVSIMPSIFGEDAVLRILDKQTLADEFHGLRVD
                     VLGFDEGTVAWLRKLACEPYGMLLVTGPTGSGKTTTLYAILSEIHNGRDKIITIEDPV
                     EYQLPGILQIPVNEKKGLTFARGLRSILRHDPDKIMVGEIRDPETAQIAVQSALTGHL
                     VFTTVHANNVFDVIGRFMHMGLDPYSFVSALNGVAAQRLVRIACTQCSIEERPAAALL
                     AASGVTAEQAEHWRFRTGKGCGQCRGTGYRGRKAIAETLILNDEMRELISSRQPVRAV
                     KEAARKLGTRSLREAALDLVACGETTLEEVNRVTFVA"
BASE COUNT          841 a         1625 c         1530 g          917 t
ORIGIN      
        1 atggcgacct ggtcatcgag gtaaacgttg ggctcgacgt cgagcttgag tccaacgtcc
       61 agatactgca ccgactcgga cacgccgacg ttcgcggtcg acgtggtcgt gattaccgga
      121 agccggtcgc cgatgtggac cttcgccttc tcgcggttct tcacccggat gcgcgggttg
      181 gcaagaaggt tggtgtcgcc gtcctgacgc ttcagattga ggacaaaggc cggatcggtg
      241 atactgaggt tcaccaggcc cgagctgcgg ttgagccatt ccgtgagcgt gatctggccc
      301 ggtttgccgg ccgcgccctc tacaccggcc gaaatctgcg tcggatattg caaccccagg
      361 ttctgcatct tgctgcgcgt aacctcgagc acctcgagct cgagtatgac ttcgggctcg
      421 gcaaggtcct gggccgcgat cagcttctcg gcgagccgta tcgcatccgg cgtgtcgcgc
      481 atgaccagca ggttcagccg ctcatccaca aacacgtcgc gggtcttgac caccgtcttg
      541 atgagattca gcgtctgctt cacgtcggca ttgccgaggt agaagctttt caccaccagt
      601 tcctggtagt cgcgcgtctt gttgggcaga ttcgggtaga gcaggatggt gttctcgttg
      661 agcaccttct tctcgagttg gttagtcacc agcaggaagt tcaccgcatc ctctatggag
      721 gtgtcgcgaa cgtagatagt cgcccgcagg tccgggcgca cgtcgcggtc gaacacgaag
      781 ttgatgccgg aggtgcgcga caggacctcg aatatgcttt tcagcggcgc atcacgaaac
      841 tcaagcgtga ccggtttcct gaattcgggt ttgagccgcg gtgccgcgac gaccgatctg
      901 gcccggctct cctcgatgcg gcgctgcagc gcccgcgcat ccttttgcga cggattactc
      961 ccgagcacct tcctcagtaa ttcaagcgcc tcctccggtt ttccctcatc gaacatcgcc
     1021 tgcgcctgtg caaccgccgc gcgctggctt tgctcggccc gggccgcctc cagcccggct
     1081 tgcgcgcgga cattgccggc acttaactcc agcacgcgct ggaacgccgc gatcgctcct
     1141 tcgctgcgac cgagcatgcg cgccttttcg ccttcataga gtagctggtt gacgtacaga
     1201 tcgcggttgc gcgcgtaata gttgcgcaac tcggtgtcgt ccggattcgc cttgaccccg
     1261 cgttccatgc ggcgaatggc ctcttccgtg cgtccctgct cgaggagttt tcggccttcg
     1321 atcattgcgg tatgcgcggc gcagcccgca gcgaaaatca gcaagaagac aaccgccagt
     1381 tttctcactg cggctctccg atgatgatag tctggcgctg gttgagcggc aggaacagca
     1441 gggtgatgcc accttcggcg atgcgttcga ccttgtaaag agaatcgatc gtgtcgcctt
     1501 catgcacgat caggttgcgc tcgccctgga ccagaaagac ggcgagatcg cctccttgca
     1561 ccagcttgcc catataggta aatggcagcg gcggcgcgct cagcggcggt ggactcggcg
     1621 ccggcgcaac tacgacctgc gcggggggcg ccgcggcggc gggtttcgat ttggccgcac
     1681 gcggcgcccc tgtcgtgaac ggatcccgta tcgcccttcc gagttcgcgg gatttcagct
     1741 tttcgagatt gatggccggt gcttcgccgt ctgttccggc agccgacggc cggggcgccg
     1801 gcgccgcgct ggaatccgaa gccgcgacga cctccgtccc ggaggggacc gcactgtccc
     1861 ggatccaggc ggccgcggcc agcgtcgcag tcaacaggcc agcgacaacc agccagcgct
     1921 gacgggtgtg aagtttcatg cggcagatct catgaagacg gtgaatcgca attgcacctc
     1981 cagcgccggg gagccgccct ggccacgctg catgctgatc gcgtcgaggg caaggcccgg
     2041 cgtgtcgtgc atcgcctgcg taaggaaacg cttcacctgc ggataacttc ccttcacggg
     2101 taccacgact tgatagcgca tcagcttgcc cgacgggtca ggcagcggcc ggtattcgcc
     2161 gcgttcaaga accaggccgg cgtcacgggc gtaggagtgc agccggcgca cgacttccgg
     2221 cgcctgttct gccgcaggca actgttcgta gaaggcggtc ataccggcat cggggcgtcc
     2281 cacctcgacg atccgtgctt gatttcctgc ccgcaattcg agtcgctgca cgcgatcgcg
     2341 cagtgccgtc acgtcggcct gcaacggcat gaggttcgac gcgacgaagc cggcggcaag
     2401 gatcgccagc gcgacgccgc cgatggcggc ccagcccatg gcatgcaggg cgcgctgcag
     2461 ccgccagcgg atccgtttca gcgcggcatc catgacgcgc tcaccaggaa acggatcggc
     2521 cgcacggcgt tttgccgttc gcgttcatgc tgcagcagat agacctgcga aagttcgctt
     2581 tgctgctcga gccgcctgac gtagtcgaac atggtctctg cgtcggacgc ctccgcactg
     2641 atctgcacga tccccgactt cgcatccggg acgatgccga gcagcgcgac ccggtcggtc
     2701 gcggcagcct cgaccgagcg gaacaatctg tcccacggca gcgccagccg gtcgatcatt
     2761 tcgttaaccc ggcggatttc ctgcttgagg gactcgtcca cccgtggccc ggccggcgcg
     2821 agtccgcgcg ctctgcgctc gagtttcgac gcctgcgact cgagatgtcg tgcttcgctt
     2881 ttgagatcgg cgtgaaacga caacacgacg aatgcggcca ccacagccgc ggccagcagc
     2941 actatcccgc ctccggagga gcggcgtgca gcctgcagga aatcgagttg cacggcacgc
     3001 atggtcatag tccgcacatc gccagcgcga ggccggcatt ggctcccgcg gccctggcca
     3061 agggcgccgg gagcgcaagc cgctcgacct tcaatccatc ggtctccacg gcgacattgc
     3121 ccagctttgc caggaacact ttattggcgc taccggccga aagcagaagc tgctcctgct
     3181 cgagcacctg cgccagggag accgaagcgt cgtgcagcgg acgcacgcgc accgaacgcc
     3241 actggcccgc ggaaagccac gccacgagca gccgcccttg ctcggcgatt accagccagc
     3301 cgtcggctcc gatccggcgc cgccactcgt tgaactgcgc catcaacgcc ggctgcacgg
     3361 actgcaggcg caatccggag gccgcgaccg cgccggtggc ggcaccgatc agggcctggt
     3421 ccacggctgc gcacaaacga ctggcgccgg ccggtgccgg actcatcgcg atcgtccaat
     3481 cgcgtgccga ctgaccgaat acctggatga agcgcgtgcg cgcgaattca agttcttcgg
     3541 cctcggtgac gagctcgctg ctccagggca gcacgacgta acgaacgaaa tggctcgaca
     3601 ggattagcgt agcgtcgccc ttgtccacgt tcgggtgagg cagaagatcc cgcaagactt
     3661 ccaccgcgcc agcccagttc tcgtgcccct tggcttcggc gcagggcatg gtcttgcgat
     3721 cgacgacgcg cggcccgagt ccgcgcgaaa accgcaccat tgccacctgg tgaggtgaca
     3781 gggcgatgcg gacgcgctca cgccacgaaa gtgacacgat tgacctcctc gagggtcgtt
     3841 tccccgcagg cgaccagatc aagggcggct tcgcgcaggc tgcgggtacc gagcttgcgc
     3901 gccgcctcct tgactgcacg caccggttgg cgcgagctga tgagttcgcg catttcgtcg
     3961 ttgaggatga gcgtttccgc gatcgccttg cggccacggt agcccgtgcc gcgacactgg
     4021 ccgcagcctt tgccggtgcg aaaccgccag tgttcggcct gttccgcggt aacacccgag
     4081 gcggcgagca gcgccgccgc cggccgttcc tcgatcgagc attgcgtaca tgcgatcctg
     4141 accaggcgct gtgccgcgac cccgttcagc gcgctcacga aactgtaggg atccaggccc
     4201 atgtgcatga accggccgat cacgtcgaag acgttgttgg cgtgcaccgt ggtgaaaacc
     4261 aggtgtccag tcagcgccga ctgcaccgct atctgcgcgg tttccggatc gcggatctcc
     4321 cccaccatga tcttgtccgg gtcgtgccgc agaatcgagc gcaagccgcg cgcgaaggta
     4381 agcccctttt tttcgtttac gggaatctgc aggattcccg gaagctggta ttcgaccgga
     4441 tcttcaatgg tgatgatctt gtcccgtccg ttgtggatct cggacaggat ggcataaagc
     4501 gtagtggtct tgccgctgcc ggtcgggccg gtcaccagca gcatcccgta aggctcgcac
     4561 gctagcttgc gcagccacgc cacggtgcct tcatcaaagc ctagtacgtc caccctcagt
     4621 ccgtggaact cgtcggctag ggtctgcttg tcgagaatgc gcaggaccgc gtcctctccg
     4681 aagatggacg gcatgatgga gacacggaag tccacctcgc ggcccttgac ccgcacctta
     4741 aagcggccat cctggggaat ccggcgttcg gcgatgtcga gctcggccat gactttcaca
     4801 cgcgacacga tctgctccgc catgtccgcg ccctgcgcga cgccgacctg gctcagcacc
     4861 ccatcgatgc ggtatttaat cgcgagccgg ttgcccgccg attcgagatg aat
//
LOCUS       DASQOP010000152         2176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1270088, whole genome shotgun sequence.
ACCESSION   DASQOP010000152 DASQOP010000000
VERSION     DASQOP010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270088"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..123
                     /locus_tag="VE030_06295"
     CDS             28..123
                     /locus_tag="VE030_06295"
                     /inference="COORDINATES: protein motif:HMM:NF018933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23061.1"
                     /translation="MCGAIHDRNVNAAHNILAAGHRRLAEGILGP"
     gene            complement(144..290)
                     /locus_tag="VE030_06300"
     CDS             complement(144..290)
                     /locus_tag="VE030_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23062.1"
                     /translation="MELGILTYDVQPFGARSVALGYQIDFSRSYDVIGEVGMLSVQRE
                     TGCQ"
     gene            744..>2176
                     /locus_tag="VE030_06305"
     CDS             744..>2176
                     /locus_tag="VE030_06305"
                     /inference="COORDINATES: protein motif:HMM:NF012531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HZF23063.1"
                     /translation="MIASLDDQASHWVLQTAISSLNRLTHRGAIAADGKTGDGCGLLI
                     KQPTAFLQAAAAEAGFKLAPLFAAGLVFLNRDPELAGQARRILTEQLQLEKLQVAGFR
                     VLPVDSQACGPEALKTLPAIEQIFVNAGGDLDEATFNRKLFVARRRTEKKLEAGDPIF
                     YIPSLSASTIVYKGMVMPQFLAQFYPDLEDPRMEASVVVFHQRFSTNTMPQWRLAHPY
                     RYLAHNGEINTVQGNRSWAVARGPLFRSPVLPDLTDILPVVSLTGSDSQSLDNMLELL
                     LVGGLDPMHAMRMLVPPAWQSVDMIDPDLKAFYEYYATHMEPWDGPAGIVLTDGRYAC
                     CTLDRNGLRPARWVITKNRHITIASEAGVWDYNPEDVVKKGKLGPGEMLALDLQTAEL
                     MDTKRVDDLLKSRHPYKAWLKKGVRYLQTDLIDPRLAAEPFDRDTLAVFQKMYNVSAE
                     ERDEIIRVLAEDESEAVGSMGDDTPMPV"
BASE COUNT          417 a          655 c          683 g          421 t
ORIGIN      
        1 cttggaataa ggggatggca gtgcactgtg tgcggagcga tccatgatcg taacgtcaat
       61 gcagctcaca atatcctcgc ggcgggacat cgccgtcttg cagaaggaat cctcggccct
      121 tgagggcgag gaggatgtca acatcattgg cagccagtct ctctctgcac ggaaagcatc
      181 ccgacctcgc cgatcacatc gtacgatcgg ctgaagtcga tctgatagcc aagcgcgacg
      241 cttcgcgcac cgaatggttg aacgtcatag gtcaagattc caagttccat gaccagtcgg
      301 ttactacgct gaccagtcga cgaaactact gccacgcttc cgaaaggttg gaaaggccgg
      361 ctatctgccc ccaggctgac tttagcgcgc ggagggcgcc gaacgctcac tgacggtccc
      421 agcggcagcg gcggagacga cgccctcaat gaaacggcga ttgacggtga ttaccacgta
      481 ggtattatgg ttcgcataca caggcgcgcc taacggcgtt tgccccacct tggcgctcga
      541 cgttcgagtg ccttgaaccg gtttaagttg ttgtttttaa aaggctctgc gggtacctcg
      601 cagggcgggc tttagcatgg gcgcaagata tggcagagcg agacactgca gcgtttgaaa
      661 ccagccgggg cggcggggac ccgcgtattg gggattgggg tctttaccgg tcggcctatg
      721 agcgggattc ctgtggattc ggcttgatcg caagtctcga cgatcaggcc agtcactggg
      781 ttttgcagac ggccatcagc tccctcaatc gcctgactca ccgcggtgcg attgccgcgg
      841 acggcaagac cggggatggc tgcggtctct tgatcaagca gccgactgct tttctgcagg
      901 ccgcggccgc ggaggccggg ttcaagctgg ccccgctgtt tgccgccggc ttggtgttcc
      961 tcaatcggga cccggaactt gcgggtcagg cgcgaaggat tctcaccgag cagctgcaac
     1021 tcgagaagtt gcaggtcgcc ggcttccgtg tcctgccggt ggacagccaa gcctgtggtc
     1081 cggaggcgct caagaccctg ccggccatcg agcagatttt cgtgaatgcg ggcggcgacc
     1141 tcgatgaggc caccttcaac cgcaagctgt ttgtggcgcg tcggcgcacc gaaaagaaac
     1201 tggaagccgg cgacccgatc ttttatatcc cgtccttgtc ggccagcacc atcgtctaca
     1261 agggcatggt gatgccgcag ttcctggcgc agttctatcc cgacctcgag gatccgcgga
     1321 tggaagcgtc ggtggtggtt ttccatcaac gcttttccac caacaccatg ccgcagtggc
     1381 gcctcgccca tccctaccgg tacctcgccc acaacggcga gatcaacacg gtgcaaggca
     1441 accggagctg ggcggtggcg cgcggtccct tgtttcgctc gccggtgctg cctgatctca
     1501 ccgacatcct gcccgtggtc tcattgacgg gttcggattc gcagtcgctc gacaacatgc
     1561 tcgaactctt gctcgtgggc ggcctcgatc ccatgcatgc gatgcgcatg ctggtgccgc
     1621 cggcgtggca gtcggtggac atgatcgacc cggacctcaa ggcgttctac gagtactacg
     1681 ccacgcacat ggaaccctgg gacggcccgg ccggcatcgt gctcaccgac ggccgctacg
     1741 cctgctgcac cttggaccgc aacgggctgc gtcccgcgcg gtgggtcatc accaagaatc
     1801 ggcacatcac cattgcctcg gaagcgggcg tctgggatta caaccctgag gacgtggtca
     1861 agaagggcaa gttggggccg ggcgagatgc tggcgctcga cctgcagacc gcggagctca
     1921 tggacaccaa gcgagtcgat gatctcttga agagccgcca tccctacaag gcgtggctca
     1981 agaagggcgt gcggtatctg caaaccgatc tcatcgatcc caggctggcg gcggaaccct
     2041 tcgatcgcga caccctggcg gtgttccaaa agatgtacaa cgtgtcggcc gaggagcgcg
     2101 acgagatcat tcgcgtgttg gctgaagacg agagtgaagc ggtcggctcg atgggcgatg
     2161 acacgccgat gccggt
//
LOCUS       DASQOP010000153         2848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2277114, whole genome shotgun sequence.
ACCESSION   DASQOP010000153 DASQOP010000000
VERSION     DASQOP010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2277114"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..477)
                     /locus_tag="VE030_06310"
     CDS             complement(67..477)
                     /locus_tag="VE030_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZF23064.1"
                     /translation="MKFIQDNIWLVLIALASGGVLVWPWISRLLSGAPEVGPLEAVQL
                     INRKDAMMLDVREAGEFNAGHAPNAKNVPLGQLDKRLGEIAKFKARPVVVTCQTGSRS
                     HAATSALKKAGFGEVVVLSGGFGAWQQANLPVEK"
     gene            complement(474..2330)
                     /locus_tag="VE030_06315"
     CDS             complement(474..2330)
                     /locus_tag="VE030_06315"
                     /inference="COORDINATES: protein motif:HMM:NF023274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy polymerase domain-containing protein"
                     /protein_id="HZF23065.1"
                     /translation="MASAAAHCRQQSLLYPNSAWKGPDLTNSYTGAATPFAARLALVL
                     VGLAWVLPFLSPNFREPISSFYGEATAAVIGLAAFACVLSRSLWAGIELPRSSLVFLG
                     FAGLILLHVLLGRSVYLQQNLLAVLYLLWATALAMLAWRLKQMLGMETVVVALCWFVV
                     AGALANALIGLLQLWGVQSPLSPYVLPPVNGRIFANTGQPNHLANYLCLGLASLAYLY
                     GVRRLALPLALAAAAPLLLVLTASGSRSAWLYLIALVILGVVLQRLRPQPAASRILGF
                     GIACVGGFLATQWFAEFLFPATEFPVVTMRTRLGVEGMQSADRLRLWYEAWRMFLDAP
                     FFGMGFRQFAWQHFLLNAQLPAPATERGIADNAHNLLFQTIAEFGIAGVAVLCAGLAS
                     LLLAMRRQEPSVHLWWLCAVLAILGIHSMLEYPLWYAYFLGIAAVALGVSETVAVKVG
                     DRSRGRLLLVLMLLLGCMAAANIYQDYRTLQSLHRIEPHPSGATARSGESTAAVLLEL
                     QQHSLFAPFVELALSRLIVLNREQIDDKLALNGLVMRFAPSADVVYRHAILLALKGEQ
                     QEARAQWDLAEANYLSDRGAVIRIMETIAAGGEKGVQDLIRYTTTGSAKEPK"
     gene            2423..>2848
                     /locus_tag="VE030_06320"
     CDS             2423..>2848
                     /locus_tag="VE030_06320"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HZF23066.1"
                     /translation="MKKIVLLRHGESVWNRENRFTGWTDVDLSDQGVVEAREAGRLLK
                     EAGFGFDLALGSVLKRAIRTMWIVLDEMDLMWVPQQLSWRLNERHYGALQGLNKAEMA
                     AKYGEGQILVWRRSYDVAPPPLARSDPRYPGNDPRYRDMK"
BASE COUNT          593 a          929 c          877 g          449 t
ORIGIN      
        1 gagcagccgt tccgcggcaa tgcagtaggg acaccaggcc gtgcagtaca tgctgatccg
       61 cggcatctat ttctccaccg gaagattggc ctgttgccag gctccgaagc cgcctgacaa
      121 caccaccacc tcaccgaagc cggctttctt gagcgccgaa gtggcggcat gcgagcggct
      181 gccggtctgg caggtcacga ccaccggccg ggctttgaac ttcgcgatct cgccgaggcg
      241 cttgtcgagc tggcccagcg gcacgttttt cgcgttcggt gcatgtccgg cgttgaattc
      301 gcccgcctcg cgcacatcca gcatcatcgc atccttgcgg ttgatcagct gcaccgcctc
      361 cagcgggccg acttcgggag ccccgctcag aagccggctg atccacggcc agaccagaac
      421 gccgccgctc gccagcgcga tcagcaccag ccagatattg tcctgaatga acttcacttc
      481 ggttcctttg cgcttcctgt cgttgtgtat cggatcagat cctgcacgcc cttttcaccg
      541 cccgccgcta tggtttccat gatccggatc actgcgccgc ggtctgacag gtaatttgcc
      601 tccgcaagat cccactgggc ccgcgcctcc tgctgttcgc ccttcaatgc aagcagaatg
      661 gcatgccggt agaccacatc cgccgacggc gcaaaccgca tgaccagtcc gtttaacgcc
      721 agcttgtcgt cgatttgctc tcggttaagc actatcagac gcgacagcgc caattccacg
      781 aagggagcga acagagaatg ctgttgcagc tccagcagaa ccgccgccgt gctctcgccg
      841 ctccgagccg tggcgccgga tgggtgcggc tcaatccggt gcagcgactg gagggtgcga
      901 tagtcctgat aaatattggc tgccgccata cagccgagga gcaacatcag caccagcagc
      961 aaccggccgc gactgcgatc gccgaccttc accgcaacag tctcggacac gccgagcgca
     1021 accgctgcaa tgccgaggaa ataggcgtac cagagcgggt actctagcat actgtggatg
     1081 cccagaatcg cgagcaccgc gcacaaccac cacagatgca cgctcggttc ctgacggcgc
     1141 attgcgagca gcagggacgc cagtccggcg cacaggactg ccaccccggc gatgccgaat
     1201 tcagcgatgg tctggaacag cagattgtgc gcattgtcgg cgatccccct ctccgtcgcc
     1261 ggcgctggca attgtgcgtt gagcaggaaa tgctgccagg caaactgccg gaatcccatg
     1321 ccgaagaatg gcgcgtcgag aaacatgcgc caggcctcgt accaaaggcg cagccggtct
     1381 gcggattgca tgccctcgac gcccaggcgc gtccgcatgg ttacgaccgg aaattccgtt
     1441 gccgggaaga ggaattcggc gaaccactgc gttgccagga agccgccgac gcaggcgatg
     1501 ccgaagccga ggatgcggga agccgccggc tgcggccgca gccgctgcaa caccacgccg
     1561 aggatgacca gcgcaatcag atagagccag gcgctgcgcg agccggacgc cgtcagcacc
     1621 agcagcaacg gcgccgctgc ggcaagggcg agcggcaagg ccagccgccg cacaccgtag
     1681 agataagcca gcgaggcgag cccgaggcac aggtaattgg ccagatgatt gggttgcccg
     1741 gtattggcga agatgcggcc attgactggc ggcagcacat agggcgaaag cggtgactgg
     1801 accccccaca gttgcagcag tccaatcagt gcattcgcca gcgctccggc aaccacgaac
     1861 cagcacaacg ccacgaccac ggtctccatc ccgagcatct gcttgagccg ccacgccagc
     1921 atcgccagcg cggtggccca caataaatac agcaccgcca gcagattttg ctgcagatag
     1981 accgagcgtc cgagcaggac gtgcaaaaga atcagcccgg caaagccgag aaaaaccagg
     2041 ctcgaacgcg gcaattcgat gcctgcccac aacgaacggc tgagcacgca cgcgaatgcg
     2101 gcgagtccga tgaccgccgc agtcgcctcg ccgtagaaac tcgagatggg ctcgcggaaa
     2161 ttggggctga ggaacggcag cacccaggcc agtccgacca gtaccagggc aagccttgca
     2221 gcgaacggag tagcggcgcc ggtgtacgaa ttggtcaggt ctggcccttt ccaggcactg
     2281 ttggggtaaa gaaggctttg ttgccggcaa tgcgcggcgg cggatgccac gaccggcgcg
     2341 acacgcgcgc aagccgatac tacaattcta tcttcgaagc gcgcgcttcc gtgcgcgcag
     2401 aattcaagat cgatcgcacc ggatgaaaaa aatcgttctg ctccgccacg gcgaaagcgt
     2461 gtggaacagg gaaaaccgct ttaccggctg gaccgatgtg gacctgtcgg accagggcgt
     2521 ggtcgaggcg cgcgaggccg gccggctgct caaggaggcc ggattcggtt tcgacctcgc
     2581 actgggctcc gtgctcaagc gcgccatccg caccatgtgg attgtcctcg acgagatgga
     2641 tctgatgtgg gtcccgcagc agctctcctg gcggctcaat gagcgtcact acggcgcgct
     2701 gcaggggctg aacaaggccg agatggcggc gaagtacggc gaaggccaga ttctggtgtg
     2761 gcgccgcagc tacgacgtcg cgccgccgcc gcttgcgcgg agcgatccgc gctaccccgg
     2821 aaacgacccg cgctatcgcg acatgaag
//
LOCUS       DASQOP010000154         3012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2481510, whole genome shotgun sequence.
ACCESSION   DASQOP010000154 DASQOP010000000
VERSION     DASQOP010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2481510"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VE030_06325"
     CDS             complement(<1..336)
                     /locus_tag="VE030_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZF23067.1"
                     /translation="MNPSPYVPPPSIIALVVLLALGWGVNWPVMKLGVQQLPPIFFRA
                     ICIAGGLVVLWLYARVAGISLAVPRRAWRDVVRLALPNVIIWHLLVVVALKILPAGRA
                     AILGYTMPVW"
     gene            434..1189
                     /locus_tag="VE030_06330"
     CDS             434..1189
                     /locus_tag="VE030_06330"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HZF23068.1"
                     /translation="MLYLYGLLSSLPIWRYCIAQITLDIDCFPCRILFVAVQYIHRSS
                     TNAPQNKSTTEITASIPPSAVLITEMIMNFTPEQIASTNKAGIEAVLGLAHTQFAAFE
                     RLSALYFNATKAAFEEGVGHAKALLNAKDPQEYLNLNAAATQPTMEKAIAYSRSLYEV
                     AAQTNGELAKFLEAQAAEFNKNLVGLLDKVSKNTPAGSDVAVAAVKSALAAANTAYES
                     FNKVAKQATEIAEANFAAATSATKESKKQRATA"
     gene            complement(1336..2085)
                     /locus_tag="VE030_06335"
     CDS             complement(1336..2085)
                     /locus_tag="VE030_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HZF23069.1"
                     /translation="MRVGAIVIGEEIIRGKREDKHFPKLISILGSRGMRLHWCEYLGD
                     MPDLITATLKRTFASGDLVFSFGGIGATPDDHTRQCAADALGLELLLHPDAEAAIRSR
                     TDMEITPQRLKMGEFPRGSRIIPNPYNRIPGFSLEDHHFVPGFPVMAWPMVEWVLDHR
                     YADLFHLTPEAEAAIIVYDLPESTITPLMLEVESSFRGLKSFSLPSMGEQGVRRHIEL
                     GVRGAPEEVTPAIEQMKLGVAALGGRWEEKP"
     gene            complement(2082..2828)
                     /locus_tag="VE030_06340"
     CDS             complement(2082..2828)
                     /locus_tag="VE030_06340"
                     /inference="COORDINATES: protein motif:HMM:NF018917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HZF23070.1"
                     /translation="MNAILNALARALVSLMHPRMLWLMVWPVLVALVIWVALAALYWS
                     EAAKWIDFQLHQWPVYEWAVSIWPFTLVAAWFGWLLLLLLFVPAVLITAVLIISVVSM
                     PAMAAHVGERDYPGLARRKGGTLAGSVWNAVAALVLFGLLFAVSLPLWLVPPLWPVLP
                     VVLFGYFNQRVFRYDALAEHATAAEIAAVIRRSRGELFLLGVALALIGHIPLVGFLMP
                     VYGGLAFVHYGLARLGELRSEPIQGTARRV"
BASE COUNT          664 a          992 c          864 g          492 t
ORIGIN      
        1 ccacaccggc atcgtgtagc cgaggatcgc ggcgcgtccg gccggcagga tcttcagcgc
       61 aaccacaacc agcaagtgcc agatgatcac attgggcagc gcgagccgca ccacgtcgcg
      121 ccacgcgcgc cgcggcacgg cgagcgaaat cccggcgacc cgtgcataca gccacagcac
      181 aacaagaccg ccggcgatgc aaatggcacg aaagaaaatc ggcggcaact gctgcacgcc
      241 cagcttcatc accggccagt tgactcccca gccaagggca agtaggacaa ccagggcaat
      301 gatcgaaggg ggcggtacgt agggactcgg gttcacgatt ctgccttgca tccggagtaa
      361 atggacagac tctttttaaa acaaatagtt ggatgtaggt gtccactgac ttctaccgtg
      421 gcagcccact gcaatgctct atttatatgg acttttatcg tcccttccca tatggcgcta
      481 ttgcatcgca caaattaccc ttgacatcga ttgttttcct tgtagaatcc tgtttgttgc
      541 agtgcaatat atccaccgaa gcagcaccaa cgcaccacag aacaagagca ctactgagat
      601 aacggcaagc attccaccta gcgcagtttt aattacggag atgattatga acttcacgcc
      661 cgaacaaatc gccagcacga acaaggccgg catcgaagcc gtcctaggtc tcgcccacac
      721 gcagttcgcc gctttcgagc gcctgtccgc gctgtatttc aacgcgacca aggccgcgtt
      781 tgaagaaggc gtcggccacg ccaaagcgct gctcaacgcc aaagaccccc aggaatacct
      841 caacctgaac gccgcggcaa cccagccgac gatggaaaaa gccatcgcct actcgcgcag
      901 cctgtacgaa gtcgccgccc aaacgaacgg cgaattggcc aagttcctcg aagcgcaagc
      961 cgccgagttt aacaagaacc tggtcggcct tctcgacaag gtctcgaaga acacgccggc
     1021 cggttccgac gtcgccgtcg cggccgtgaa atccgcgctt gccgcggcca acaccgccta
     1081 cgagagcttc aacaaggtcg caaagcaagc caccgagatc gccgaggcga atttcgccgc
     1141 ggcgacttct gccaccaagg aatccaagaa acagcgcgcg acagcctgac ctgccggctc
     1201 ctcgcgtcca cggtcccctc ctcctctccg tgggcgccct aaagccctgg cgcaagccag
     1261 ggctttttgt tttggggaca gaccacgttt ttcgtcaagc cgccgaaggg aaaaacgtgg
     1321 tctgtccccg gtttactaag gcttttcctc ccacctgccg ccgagcgcgg ctaccccaag
     1381 cttcatctgc tcgatagccg gcgtcacttc ttccggcgcg ccccgcacgc cgagttcgat
     1441 atgacggcgc acgccttgtt cgcccatgct gggcaggctg aaggacttca gcccgcgaaa
     1501 gctcgactcg acttccagca tgagcggcgt gatcgtcgat tccggcaagt catagacgat
     1561 gatcgcagcc tcggcttcgg gggtaaggtg aaacaggtcc gcatagcgat ggtcgagcac
     1621 ccactcgacc atgggccagg ccatcaccgg gaatcccggg acgaaatggt gatcttccag
     1681 ggaaaacccc gggatgcggt tgtacggatt gggaatgatg cgcgaaccgc gcggaaattc
     1741 gcccattttg agccgctgcg gcgtgatttc catgtcggtg cgcgagcgaa tcgccgcttc
     1801 cgcatcgggg tgcagcagca attcgaggcc gagcgcgtcc gccgcgcact ggcgcgtgtg
     1861 atcgtccggc gtcgcgccga tgccgccgaa gctgaagacg aggtctcccg aagcgaacgt
     1921 gcgcttgagc gtcgcagtga tgagatccgg catgtcgccg aggtactcgc accagtggag
     1981 cctcattccc cgcgagccga ggatcgagat cagcttgggg aaatgcttgt cctcgcgctt
     2041 gccgcgaatg atttcctcgc cgatgacgat cgcgccgacc ctcacacccg cctcgccgta
     2101 ccctggatcg gttcgctgcg cagctcgccc agtcgcgcga ggccgtagtg gacgaaagcg
     2161 agcccgccgt acaccggcat gagaaacccg accaaaggta tgtgcccgat gagtgcgagc
     2221 gctacaccca gcaggaacag ctcgccgcgg ctgcgtctga taaccgcggc aatttcggcg
     2281 gcggtcgcgt gctcggcgag agcgtcatag cggaacacgc gctggttgaa atagccgaac
     2341 aggacgacag gcagcaccgg ccacaacggc ggaaccagcc acagcggcag gctgaccgcg
     2401 aagagcagcc caaacaggac gagcgctgcg accgcgttcc agacgctgcc ggccagcgtg
     2461 ccgccctttc tccgggcgag ccccggataa tcgcgctcgc cgacgtgagc ggccatcgcc
     2521 ggcatggaca cgacgctgat gatcaacacc gccgtgatca aaaccgcggg tacgaacagg
     2581 agcagcaaca gaagccatcc gaaccacgcg gcgactagcg taaagggcca gatcgagacg
     2641 gcccattcat agaccggcca ctggtgcagc tgaaagtcga tccacttcgc cgcctcgctc
     2701 cagtacagcg ccgcgagcgc cacccagatg accagcgcga cgagcaccgg ccacaccatg
     2761 agccacagca ttctcggatg catcaggctc accagcgcac gcgccagggc attgagtatc
     2821 gcgttcatat ggaccgcccg aggcggtgga aatcagcgtt gaatagtttg ccacatcttc
     2881 gacagccgct tcaccgagac cggcacggag gtcttcagct cctgggcgaa aagctgaacg
     2941 cgcagctcct cgagcaacca ccggaactgc tcgagctgcg ggtcgcggtt gcccgacttg
     3001 agctgcctcg cc
//
LOCUS       DASQOP010000155         8828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_663442, whole genome shotgun sequence.
ACCESSION   DASQOP010000155 DASQOP010000000
VERSION     DASQOP010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8828
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663442"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..108)
                     /locus_tag="VE030_06345"
     CDS             complement(<1..108)
                     /locus_tag="VE030_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HZF23071.1"
                     /translation="MTATLIDGVAVSKAIRAQWQQRVGALKARGVAPGLA"
     gene            complement(198..2315)
                     /locus_tag="VE030_06350"
     CDS             complement(198..2315)
                     /locus_tag="VE030_06350"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZF23072.1"
                     /translation="MGSIKEAVENRLRVARVACPHDCPDACLMRVTVDTLSGRAIKVE
                     GDPTHPVTRGYLCNKVNHYLDLVYNDRRVLFPHRRTGPKGPGAKFLRITWDEALTEVA
                     ARLKAVIAEHGAEAVQPFSYSGTLGILGFLGMGERFFNKMGAARLERTICTAAGAAAE
                     MHTFGRVGDANIEDLPQMDLVILWGTNLVSTGVHAMPFVNAARDNGAKIIAIDPRVTR
                     TTAFADWHIQPRPGTDAALALGMMKIIVDRELHDLDFLRSHTVGWEKLLEERLPEYPP
                     ERVEAITGIAAADVEKLALLYGGTKQSFIRLNWGIQRHDNGGMMTKAIKLLPVITGAM
                     RGKGGVCMSTGGEMRRVDLRKLQRTDLLAGRKPRTINMIQLGNALNDPDLSPPVKALF
                     CWNADPANCVPDTIAARKGLAREDLFTVVHDTFWSDTASYADIVLPADTALEHVDLLA
                     AYGNYYFSLSEQAIAKQGESLDNQEMFRRLARAMGYDEPCFSQSDEDMIRELIDPEVN
                     PLFEGVSFEQLQRNGWARANTDSPRRLGVNSGKWPTPSGKIEIYSEALAELGVDPLPA
                     HVDETEGLSSAAASGRYPLQVISAATHYFIGASFQHVPRLQEMMARPTFEVSAADAGA
                     RGIGNGDYCRLYNDRGEVFGHALIVDGLLPGVIGAQKQLQGSRQRGGVNVNALISQRE
                     ADMGRGPVFFSTLAQIERVRSEE"
     gene            2412..4502
                     /locus_tag="VE030_06355"
     CDS             2412..4502
                     /locus_tag="VE030_06355"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HZF23073.1"
                     /translation="MQHFRAFLALVFAASFTFTSTAEARLTRLEISRREIVAAGMDFG
                     TAGAYEKLTGRAWFEADPTLERNAAVFDLNLAPLNGRGAVEFSADMVILKPVDMARGA
                     GTLFFEVNNRGRKISFGRMHDTASDADMNNPMAARDFGNGFLMKRGYVLAWVGWGADI
                     APGDSRLTVDFPIAQENGKPVAERILTEFSDRNFNGGKPTSLPLSGSPAFKSFPAVTT
                     DKKEAQAELYVLASDSPRPSGPGIPRGESVSDDEWAFADCPDGWPGKPSVEHICLKDG
                     FRNDRNYHLLYRATGSPVMGLGYLTSRDFVSFLRNDQKDAAGNPNPVFGIKTTLCQGI
                     SSSGMYLRDYLYHGFNVDEKERRVCDGMHIHVAGVQKLFLNYRFAQPNAFTQQHRERY
                     VPDTSFPRQYAVRINPFTNIPDGILKRPLTDPKIIHTDTSNEYWQFRASLTGASEGGT
                     RDFNESSKVRRYLLSSLQHGSFKPDAAHRGIGNRQCEQLSNPVHPGPLLRALVVALED
                     WVKKDTAPPDTKVPKVADGTLVHASELKPPKIPGLTYEGLYNGSGDRNFGPRVRGNSG
                     VIDLLIPEAVATHKVLVPQVDAIGNDIAGIRHLFVEAPAATLLGWNTRTAEFGGPDLC
                     DLLGSMIPLPKSRAEARGKGDPRPTLEELYGTHEGYVRKVAEAARKLREQRLMLSEDV
                     RLIIQEAEAGDVLR"
     gene            4589..8551
                     /locus_tag="VE030_06360"
     CDS             4589..8551
                     /locus_tag="VE030_06360"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HZF23074.1"
                     /translation="MPTDIQRTLFRRMRRWALWTAASLFLIAVLGFAYTTFVGITVDA
                     SLLRGPIATVFSDTLGRTVRFDGPMELEISARPKLRVGGLHIANAPGFEGAEFASLGE
                     ARLALDLWPLLQKRLQIEEVTGSHVRVRLQRRADGANNWTFLRARAAQQRVSEPGTPA
                     AVSADRALALLDIQQVTLENLNVEYVGPSGISHYFDLHRLSAQSPSGQPLKVSLNGAV
                     EKQFPYRLDFTGGVPADLSDPRKPWPVELTLTFLSSTLTASGTLSGRSGQIGFGLGTE
                     NLLEFERLFQTKLPAVGASGLAGTLTFEPGKVSLRQLGAAMGGTALIGDLDFDYSATR
                     PRVTGALTVPVLDLRPFLVDQPIEEAPPAPPKSLAEVYRELSQASFTLTELTRADADV
                     TLRVGRWASLPGDVSDVSLRVKIDNGQLQTPMQATVTGVKLEGSAQVDGTALPPTFRL
                     ALGTRDSQLGGLAELLAGVRGVEGKLGRFDLRLSARGDQVNELIRSLDVRLDIERGRL
                     SYGNIEGGRPVEFTLEKLSVALPAGKALTGEMRGSLLGNPVTAQLRSGALEPLMLEDR
                     VPIDFTARSGGVRAHIHGVLQLPSESRGPELNFEISAPRAGEVASWFGLRPGAEAHAA
                     LSGRAALRSRQWSLADFSLQLGRTTLTAEALHTGIGTQSLLKVRLAADQIDVAELESL
                     LPKAQKEDSSGPVLNVPILPQGIDLTDADVEVRLKRFLGSPLEVRDVSFDGRIRDGYM
                     YPSQFAANVAETALRGAILLDLRSREPNVGLWLFANNADIGNVLRKLDLARNIDATLD
                     LMQIYLASRSTRLGDLIARADLTGNFAGGRVTLRDPNTRAEARIALESGLLRAAPGDK
                     VRLDLKGSIDDIPVAIGVETASAHDLMNPKLPVPFKLSTDAAATRMELSGAIARPLGN
                     REVELALEVRGTRFDTLDKLVRASLPPWGPWSAVGKFRMSAGGYEVDDLVLQVRESAL
                     HGKGRFETGTGRPRIEIALAAPNIQLDDFRFDGWSPFEKKPANESDKTLNADELRAKA
                     TEASKQAQELLSPAMLHRQDAYLTVQVDQVLAGHDKLGSGKLVAKLENGRADIGPVEL
                     NVPGGSAKLSLGYEPTEQDVKVDTHIEVNKFDYGILARRVKPDADLRGTFSLNADVTS
                     RTRYLADILRHGSGRIDFAVWPQNMQSGIFDMWAVNVLVALIPVVDPGKASKVNCAIG
                     RFDLNDGKLVDRTILLDTGRMRVTGKGRADFGTETLNLRMRPQTKTAQFLSLATPIEV
                     SGTFRNFKISVSPGDVLETIGRLATSIIWVPLQKIAGKKIPRDGSDVCGDSLLTLPAP
                     "
     gene            complement(8560..>8828)
                     /locus_tag="VE030_06365"
     CDS             complement(8560..>8828)
                     /locus_tag="VE030_06365"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HZF23075.1"
                     /translation="IGIELAAKAGYDPNAAVSLWDKMAKLGSGSSRLDFLSTHPAPQK
                     RMETLAALVPKMMPYYLDKSPRPIYQIRASASARVVLAQCATAR"
BASE COUNT         1572 a         2901 c         2761 g         1594 t
ORIGIN      
        1 cgccagcccc ggggccacgc cgcgcgcctt gagcgctccg acgcgttgtt gccactgcgc
       61 gcggatcgcc ttcgaaactg cgacaccgtc gatgagggtg gcggtcattg gtgtgctccg
      121 tgcacaggga cgagggacga gggattacct ggatcgttct agcgtttgac ttcccctcgc
      181 ccctcgcccc tcgcccctca ttcctcactc ctgactcgct cgatctgcgc gagagtggag
      241 aaaaacaccg ggccgcggcc catgtccgct tcgcgttggc tgatcagcgc gtttacgttg
      301 accccgccgc gttgcctgga cccctgcaat tgtttctgcg cgccgatcac gcccgggagc
      361 agtccgtcga cgatgagcgc atggccgaac acctcgccgc gatcgttgta caggcggcag
      421 tagtcgccgt tccctatccc ccgcgccccg gcatccgctg cgctgacctc gaaggtcggg
      481 cgcgccatca tttcctgcag ccttggcacg tgctgaaaac ttgctccgat gaaataatgc
      541 gtcgccgcgc tgatcacctg cagcggatac cggccgctcg ccgcggcact ggacaggcct
      601 tcggtttcgt cgacgtgggc gggcagcgga tcgacgccga gttccgcgag ggcttcggaa
      661 tagatttcga tcttgcccga aggggtcggc cacttgccgc tgttcacgcc caatctgcgc
      721 ggagagtccg tgttggcgcg tgcccagccg ttgcgctgca gttgctcgaa actcacgccc
      781 tcgaacaaag ggtttacttc ggggtcgatc agttcgcgga tcatgtcttc gtccgactgc
      841 gagaagcacg gttcgtcgta gcccatcgcg cgcgccagcc ggcggaacat ctcctggttg
      901 tccaggctct cgccctgttt cgcgatcgcc tgctcggaaa ggctgaagta gtagttgccg
      961 tacgccgcga gcaagtccac gtgttcgagc gcggtatcgg cgggcagcac gatgtcggcg
     1021 taggacgcgg tgtcgctcca gaaggtgtcg tgcaccacgg tgaacaggtc ttctcgtgcc
     1081 aggcctttgc gcgctgcgat cgtgtccggc acgcagttgg ccggatcggc gttccagcag
     1141 aacagcgcct tgaccggcgg gctcagatcc ggatcgttga gcgcgttgcc aagctggatc
     1201 atgttgatgg tccgcggctt gcgcccggcc agcaggtcgg tgcgttgcag tttgcgcaga
     1261 tccacccggc gcatctcgcc cccagtggac atgcacacgc cgcccttgcc gcgcatcgcc
     1321 ccggtgataa cgggcagcag cttgatcgcc ttggtcatca tcccgccgtt gtcgtgacgc
     1381 tgtatgcccc agttcaggcg gatgaacgat tgtttcgtgc ccccgtagag cagtgccagc
     1441 ttttcgacgt ccgccgccgc tattccggtg atagcttcga cccgttcggg cggatactcg
     1501 ggcaggcgtt cctcgagcag tttttcccaa cccaccgtgt ggctgcgcag gaaatccagg
     1561 tcgtgcaact cgcgatcgac gatgattttc atcatgccca gcgccagcgc cgcatcggtg
     1621 ccgggccgcg gctggatatg ccagtccgcg aacgcggtgg tgcgcgttac gcgcggatcg
     1681 atggcgatga ttttcgcgcc gttgtcgcgc gccgcgttga cgaagggcat cgcgtgcacg
     1741 ccggttgaca ccagattagt gccccacagg atcaccaggt ccatttgcgg caggtcttcg
     1801 atgttggcat cgcccacccg cccgaaggtg tgcatttcgg ccgcggcgcc ggcggcggtg
     1861 cagatggtgc gctccagccg cgctgcgccc atcttgttga agaaacgttc acccatgccg
     1921 aggaaaccga gtatccccag cgtgcccgag tacgagaacg gctggacggc ttccgcgcca
     1981 tgctcggcga tgactgcctt cagcctggcg gcgacctcgg tgagcgcctc atcccaggtg
     2041 atgcgcagaa acttcgcccc gggtcccttg ggtccggtcc tgcggtgcgg gaaaagcacc
     2101 cgccggtcgt tgtaaaccag atcgaggtag tgattgacct tgttgcagag gtacccgcgc
     2161 gtcaccgggt gcgtggggtc gccttcgacc ttgatcgcgc gccccgacag cgtatccacg
     2221 gtgacccgca tcaggcaggc gtccgggcag tcgtgcgggc aggctactcg cgccacgcgc
     2281 agtctgttct ctaccgcttc cttgatgcta cccatcggta cctcgctcga atccggattg
     2341 tgcgatattg tacccccccg cccggccatg ccgcgcgcgc cgaagataac agaacaattt
     2401 cagaggagtt tatgcagcac ttccgcgcat tccttgcgct ggtattcgcc gccagcttta
     2461 cctttacctc gaccgcggaa gcaaggctca cgcggctgga aatcagcagg cgcgaaatcg
     2521 tcgccgccgg catggacttc ggaaccgccg gtgcctacga gaagctcacc ggccgcgctt
     2581 ggttcgaggc cgatcccact ctcgagcgca atgccgcggt gttcgacctc aacctcgcgc
     2641 cgctcaacgg tagaggcgcg gtcgagtttt ccgccgatat ggtgattctc aagcctgtgg
     2701 acatggcaag gggtgccggc acgttgtttt tcgaagtcaa caaccgcggc cgcaagatct
     2761 ccttcgggcg catgcacgac accgcgtcgg acgccgacat gaacaacccg atggcggcgc
     2821 gggatttcgg caacggcttc ctgatgaaac gcggttacgt gctggcctgg gtcggctggg
     2881 gcgctgacat cgcgcccggc gacagccgcc tgacggtcga ctttccgatc gcgcaggaga
     2941 acggcaagcc ggtagccgaa cgcatcctca ccgaattctc cgatcgcaat ttcaacggcg
     3001 gcaaacccac ttcattgccg ctgtccggca gcccggcgtt caagtctttc ccggccgtga
     3061 ccaccgacaa gaaggaagcg caggcagaac tgtacgtgct cgccagcgat tcaccgcggc
     3121 ccagcggccc cggtattcca cgcggagagt cggtaagcga cgacgaatgg gcgtttgccg
     3181 attgccccga cggctggccc ggcaaaccca gtgttgaaca tatctgcctc aaagacgggt
     3241 tccgtaacga ccgcaattac catttgctgt atcgcgccac cggctcgcca gtgatgggac
     3301 tgggttatct caccagccgg gacttcgttt ccttcctcag gaatgaccag aaagacgccg
     3361 ccggcaatcc gaaccccgtg ttcggcatca agaccacgct gtgccaggga atttcgtcgt
     3421 cgggaatgta cctgcgcgac tatctctacc acggcttcaa cgtcgacgag aaggagcggc
     3481 gggtgtgcga cggcatgcac atccacgtcg ccggggtaca aaagctgttc ctcaactatc
     3541 ggttcgcgca gcccaatgcc ttcacccagc agcaccgcga gcgttatgtt ccggacacca
     3601 gcttcccgcg ccagtacgcg gtgcgcatca atccgttcac caatattccc gacggcatac
     3661 tcaagcgccc gctgaccgac ccgaagatca tccacaccga cacgtcgaac gagtactggc
     3721 agttccgcgc ctcgctcacc ggcgcgagcg aaggcgggac gagggacttc aacgaatcgt
     3781 cgaaagtgcg ccgatacctg ctttccagcc tgcagcacgg cagcttcaaa cccgacgcgg
     3841 cgcatcgcgg catcggcaac cgccagtgcg agcaactcag caacccagtg caccccgggc
     3901 ccctgctgcg cgcattggtg gtggcgctgg aggactgggt gaagaaagac acggcgccgc
     3961 cggacaccaa ggtgcccaaa gtcgcagatg gcacgctcgt gcatgcttcc gaactgaagc
     4021 cgccgaagat ccccggactg acttacgaag gcctttacaa cggctccggt gaccgcaatt
     4081 tcggcccgcg tgtgcgcggc aattcaggcg tgatcgacct gttgattccc gaggccgtag
     4141 ccacccacaa ggtgctggtg ccgcaggtgg atgctatcgg caatgacatc gccggaatcc
     4201 gccatctctt cgtcgaggcg ccggccgcga cgctgctggg ctggaacacc cgcaccgcgg
     4261 agttcggcgg tccggacctg tgcgacctcc tcgggtcgat gattccgttg ccaaagagca
     4321 gggctgaggc gcggggcaaa ggcgacccgc gtcccacgct cgaagaactt tacggcaccc
     4381 acgaaggcta tgtgcgcaag gtcgccgaag cagctagaaa actgcgggag cagcggttga
     4441 tgctgtccga agatgtgaga ttgattattc aggaggccga agccggcgac gtgctgcgct
     4501 gatcacgtgc cgtacgcgcg agcgtattcc cccgaattga accggcactg cgcagtgcgg
     4561 tcccatcggt ttatcggata atcgcgccat gccgaccgac atccagagga ccctgtttcg
     4621 ccggatgcgg cgctgggcgc tatggacggc ggcttcgctg ttcctgatcg cggtgctggg
     4681 gtttgcttac accacctttg tcggcataac ggtcgatgcc tcgcttctgc gcgggccgat
     4741 tgccacggtc ttctccgata ccctcgggcg cacggtgcgc ttcgacggcc cgatggaact
     4801 cgagatctcc gcgcggccga aattgcgggt cggcggactg cacattgcca acgcgccggg
     4861 tttcgaggga gcagagtttg ccagcctcgg cgaggcgcgc ctcgccctcg acctctggcc
     4921 gctgctgcaa aaacgcctgc agatcgagga agtcaccgga agccacgtgc gcgtgcgcct
     4981 tcaaaggcgc gctgatggcg ctaacaactg gactttcctc cgggcgcgcg cggcacagca
     5041 gcgcgtttcc gaacccggca cacccgccgc cgtcagtgcc gaccgcgcgc tcgccctgct
     5101 ggatatccag caggtcacgc tcgagaacct gaacgtcgag tatgtcggcc cgtcggggat
     5161 tagccactac ttcgatcttc acaggctgtc cgcgcagtca ccctccggcc agccgctcaa
     5221 ggtgagcttg aatggcgcgg tggagaagca atttccttac cggctcgact tcaccggtgg
     5281 agtgccggcg gatctgtccg atccccgcaa accctggccg gtggaattga cgctcacttt
     5341 cctgagttcg acgcttaccg cgagcggcac tctgtcggga cgcagcggcc agatcggttt
     5401 cggcctcggc accgaaaacc tgctcgaatt cgagcggctg ttccagacca agcttcccgc
     5461 ggttggcgcc agcggccttg ccggcaccct caccttcgaa ccgggcaagg tctcgctcag
     5521 gcagctcggc gccgcaatgg gcggcaccgc gctgatcggc gacctcgatt tcgactattc
     5581 cgcaactcgg ccgagagtca cgggcgcgct tactgttccc gtgctggatc tgcgcccctt
     5641 tctggtcgac cagccaatcg aggaagcgcc gcccgcgccg ccgaagagtc tcgccgaggt
     5701 ttatcgtgaa ttgtcgcaag cgagcttcac cctgaccgaa ctcacgcgcg ccgatgccga
     5761 cgtcacgctg cgtgtggggc gctgggcgag cctgcccggc gacgtgagcg acgtttcgct
     5821 gcgggtgaaa atcgacaatg gccaactgca aacgccgatg caggcgaccg tcaccggcgt
     5881 caagctggag ggcagcgcgc aggtggacgg cacggccttg ccgccgacct tccgactcgc
     5941 gcttggcact cgcgactccc aactcggtgg tcttgccgaa ctgctggcgg gcgtgcgcgg
     6001 cgtcgaagga aaactcggcc gattcgatct gcgactgtcc gcccgcggcg accaggtcaa
     6061 cgaactgata cgcagcctcg acgtgcgact cgacatcgaa cgcggacgcc tttcttacgg
     6121 caacatcgag ggaggacggc cggtcgagtt cacgctcgag aaactttccg tggcgctgcc
     6181 cgccggcaag gcgctcacgg gggaaatgcg cggttcgctg ctgggtaacc ccgtgaccgc
     6241 gcaactgcgc agcggcgccc tcgagccgtt gatgctggag gatcgcgtac cgatcgattt
     6301 caccgcgcgt tccggcggcg tgcgggcgca catccacggc gtgctgcagc tgccctccga
     6361 gagccgtgga ccggaattga atttcgagat ctccgcgccg cgcgccggcg aggtggcaag
     6421 ctggttcggc ctgcggccag gagccgaggc acacgccgca ctctcgggca gggccgcctt
     6481 gcgcagccgc cagtggagcc tcgccgattt ttccctgcaa ctcgggcgca ccacgcttac
     6541 cgccgaggcc ctgcacactg gcatcggcac gcaatcgctg ctgaaagtgc gcctggctgc
     6601 cgaccagata gacgtcgcgg agctggaatc cctgttgccg aaggcgcaga aagaagactc
     6661 gtccggtccg gtgctgaacg ttcccattct gccccagggc atcgacctga ccgacgccga
     6721 cgtcgaagtg cgcctgaaac gctttctcgg gagcccgctc gaagtgcgcg acgtttcctt
     6781 cgacggccgc atccgcgacg gctacatgta cccttcgcag tttgcggcca atgtcgccga
     6841 aacggcattg cgcggcgcga tcctgctcga cctgcgcagc cgagaaccga atgtagggct
     6901 atggctgttt gccaacaatg ccgatatcgg caacgttttg cgcaagctcg atctcgcgcg
     6961 caacatcgat gccacgctcg atctgatgca gatctacctg gccagccgct ccacccggct
     7021 gggcgacttg atcgcacgcg ccgacctgac cgggaatttc gccggtggcc gtgtcacgtt
     7081 gcgcgatccg aacacccgcg ccgaggcgag gatcgcgctc gaaagtggac tgttgcgcgc
     7141 cgcgccgggc gacaaggtgc gcctcgatct caaaggttcg atcgacgaca ttccggttgc
     7201 aatcggcgta gaaaccgcct cggcacacga cctgatgaac ccgaagctgc ccgtgccgtt
     7261 caagctcagc accgatgcgg ccgcaacccg gatggaactc tccggcgcca ttgcgcgacc
     7321 gctgggaaac cgagaggtcg aactggcgct cgaagtgcgc ggaacgcgtt tcgatacgct
     7381 ggacaagctg gtgcgcgcat cgctgccgcc gtgggggcca tggtccgcgg tcggaaaatt
     7441 ccgcatgtct gcaggtggat acgaagtgga cgatctggtg cttcaggtcc gcgaaagcgc
     7501 acttcacggc aagggccggt tcgagaccgg tacggggcgt ccacgaatcg aaatcgccct
     7561 ggccgcaccg aacatccagc tcgatgattt caggttcgac ggctggtcgc cgttcgaaaa
     7621 gaagcccgcc aacgaaagcg acaagacgct taatgcggac gagctgcgtg ccaaggcgac
     7681 ggaggcgagc aagcaggcgc aggagcttct cagccccgca atgctgcacc ggcaggacgc
     7741 ctatcttacg gtgcaggtgg accaggtact ggcgggccac gacaagcttg gcagcggcaa
     7801 actggtggcg aagctagaaa acggccgcgc cgacatcggc ccggtggaac tcaacgtccc
     7861 cggcggctcg gccaagctgt cgctaggcta cgaaccgacc gagcaggacg tcaaggtgga
     7921 cacccatatc gaggtaaaca aattcgatta cggcattctg gcgcgccgcg tcaagccgga
     7981 tgccgacttg cgcggcacct tcagcttgaa cgcggacgta acctcgcgta cacgctatct
     8041 ggcggacatc ctccgccacg gcagcggacg catcgacttt gcggtgtggc cgcagaacat
     8101 gcagtcgggg attttcgaca tgtgggcggt gaacgtgctg gtcgcgctga taccggtggt
     8161 ggatcctggc aaggcctcca aagtcaattg cgcgatcggg cgcttcgatc tcaatgacgg
     8221 caagctggtg gaccggacca tcctgctcga taccgggcgc atgcgcgtca ccggcaaggg
     8281 cagggcggat ttcggcaccg aaacgctcaa cctgcgcatg cgcccccaga cgaagaccgc
     8341 gcagttcttg agcctcgcca cgccgatcga ggtcagcggc acgttcagga atttcaagat
     8401 cagcgtctct cccggcgatg tgctcgaaac gatcggccgg ctggcgactt cgatcatctg
     8461 ggtgccgctg cagaagattg ccggcaagaa gattccacgt gacggcagcg acgtatgcgg
     8521 tgactcgctc ctgacgctgc cggcgccctg actacgcggt taccgcgctg ttgcgcactg
     8581 cgctaagacc acgcgcgcac tggcggacgc ccgtatctga taaatcgggc gcggggactt
     8641 gtccaggtag tacggcatca tcttcggcac cagtgcggcg agcgtttcca tgcgcttctg
     8701 cggcgccggg tgagtggaaa ggaaatctaa gcgtgaactg ccgcttccga gtttcgccat
     8761 cttgtcccat agtgacaccg ctgcgttcgg atcgtagccg gctttcgccg ccagttcgat
     8821 gccgatgc
//
LOCUS       DASQOP010000156         5764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_664003, whole genome shotgun sequence.
ACCESSION   DASQOP010000156 DASQOP010000000
VERSION     DASQOP010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5764
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664003"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /gene="dnaG"
                     /locus_tag="VE030_06370"
     CDS             complement(<1..686)
                     /gene="dnaG"
                     /locus_tag="VE030_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HZF23076.1"
                     /translation="MIPQSFIQDLLNRVDIVDLIESYVPLKRAGSNYAARCPFHSEKS
                     PSFTVSPTKQFYHCFGCGAHGTAISFLMEYQGMGFVDAVKELAGRAGMTVPAQVPDPR
                     QKEKVGAAEDLTEVMLKAAHYYKAQLKSSERAVAYLKGRGLSGEIAACYGLGYAPDGW
                     QNLESVFADYQSKSLSEAGLVIVNEQGRRYDRFRDRIMFPIHNQRGVIIGFGGRIIDQ
                     GEPKYLNSPET"
     gene            complement(969..1415)
                     /locus_tag="VE030_06375"
     CDS             complement(969..1415)
                     /locus_tag="VE030_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HZF23077.1"
                     /translation="MTLKARITDDMKTAMRAKDAQRLSAIRLLLAAIKQREVDERKDM
                     SDTDVMSVIEKMIKQRRDSIEQFQKGGRQDLVDVEQFELSVLQAYMPQALSDAAVEAA
                     VATAIQTSGGRGMADMGKVMAVLKAQLAGRADMGKVSALVKAKLSA"
     gene            complement(1509..1721)
                     /gene="rpsU"
                     /locus_tag="VE030_06380"
     CDS             complement(1509..1721)
                     /gene="rpsU"
                     /locus_tag="VE030_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HZF23078.1"
                     /translation="MPTIRVKENEPFEVAMRRFKRTVEKTGLLTELRAREFYEKPTAE
                     RKRKLAAAIKRHHKRLRNQLLPPKLY"
     gene            1892..2914
                     /gene="tsaD"
                     /locus_tag="VE030_06385"
     CDS             1892..2914
                     /gene="tsaD"
                     /locus_tag="VE030_06385"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HZF23079.1"
                     /translation="MLVLGIETSCDETGVALYDTGTGLLGHTLFSQVTMHAEYGGVVP
                     ELASRDHVRRLLPLTRQLLERCGRRLVDIDAIAYTQGPGLAGALLVGAAVAASLGYAL
                     KVPVVGLHHLEGHLLSPLLASPAPRFPFIALLVSGGHTQLMRVDAVGEYQLLGETVDD
                     AAGEAFDKTAKLLGLPYPGGPALSQLAQSGNPKRFKLPRPMLASGDLNFSFSGLKTAV
                     LTLVRGADPDSRTKADIAASFQQAIVEVLVAKSVSAVAREGLDQLVVAGGVGANRPLR
                     EAMSKKAEREGFEVFYPPQDLCTDNGAMIAFAGALRFAAGAPGAMGFTVRARWDLEDL
                     KTVSTA"
     gene            complement(2892..3500)
                     /gene="plsY"
                     /locus_tag="VE030_06390"
     CDS             complement(2892..3500)
                     /gene="plsY"
                     /locus_tag="VE030_06390"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HZF23080.1"
                     /translation="MASALFVLAAYLIGSISFAVVVSRFFGLPDPHTYGSGNPGATNV
                     LRTGKRAAAVLTLIGDAAKGAVALWLAQQFATRFDVGESALAAVALAVFLGHLFPLYF
                     RFRGGKGVATAAGILLAMDWRIALTLLVMWVLVAVFSRYSSLASVVAAALSPLAAFHF
                     LGWGPMTWAVLVIAVLLVWRHRGNIERLLAGNESRIRLLRRS"
     gene            complement(3523..4419)
                     /gene="xerD"
                     /locus_tag="VE030_06395"
     CDS             complement(3523..4419)
                     /gene="xerD"
                     /locus_tag="VE030_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HZF23081.1"
                     /translation="MAADDGLLDEFCDALWLEDGLSRNTLESYRRDLRLFAVWLASER
                     GKTLPEAERVDLLGYLAHKFQRHAKPRSAARLLSSLKRLYQYLLREGGITADPTLQVE
                     APKLPRSLPKSLTEEDIERLLAAPDVDTPLGLRDKAMLEALYASGLRVSELVSVGVVQ
                     VSQDMGVVRIVGKGSKERLVPLGEEALAWIRRYVTQARPKILDGKRTDALFVTARGDS
                     MTRQAFWHLIRRYVAKAGLSKLVSPHTLRHAFATHLLNHGADLRVVQLLLGHADISTT
                     QIYTHVARERLKQLHAKHHPRG"
     gene            complement(4419..4895)
                     /locus_tag="VE030_06400"
     CDS             complement(4419..4895)
                     /locus_tag="VE030_06400"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HZF23082.1"
                     /translation="MLESHQARMQSPFAVLGIRVIGERLTHIEYLPRGAATLKPQTPF
                     AKEVCRQLAAYLKDPDFEFDLPFEYDGTEYQRKVWQTVHAIPPGKVLSYLQVARRIDS
                     APRPVGSACGANRIPILIPCHRVIASDGIGGFMHTRRGSPIEIKRWLLHHENPGAY"
     gene            5036..5749
                     /locus_tag="VE030_06405"
     CDS             5036..5749
                     /locus_tag="VE030_06405"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HZF23083.1"
                     /translation="MNTLDPSNISTCSLESMQLRVGDRLQLELQTDSTRSHYYTTLIG
                     YVSGHSVLIRTPQVQNLPIPVREGEPVLVRTFSGRHAYTFGSKVDRVCRTPYPYLHLA
                     YPAQVQQTLIRGALRVRVNLTATALNAANQGEALPSALTIADLSISGALLEAEKSLGE
                     VGETLELAFKFLVQPNNYEVKLATPVEIQSVRKTRRQRRPEEVFTHGVRFAKLRSTEG
                     LLLQSYVQQVLLDDRSRVV"
BASE COUNT         1055 a         1813 c         1811 g         1085 t
ORIGIN      
        1 gtttccggcg agttcagata cttgggctcg ccctgatcga tgatgcgccc gccgaagccg
       61 atgatcacgc cccgctgatt gtgaatcgga aacatgatgc ggtcgcggaa acgatcgtag
      121 cgccgcccct gctcgttcac gatcaccagc ccggcttcgc tcagcgattt cgactgatag
      181 tcggcgaata ccgactccag gttctgccag ccgtcggggg cgtaaccgag gccgtagcag
      241 gcggcgattt cgccgctgag cccgcggccc ttcagatacg ccaccgcccg ctccgaggac
      301 ttgagctgcg ccttgtagta atgcgccgcc ttgagcatga cttcagtgag atcctcggcg
      361 gccccgacct tttccttctg gcgcgggtca gggacctgtg cgggcacggt catgccggca
      421 cgaccggcca gttccttgac cgcgtcgaca aaacccatgc cctggtactc catcaaaaag
      481 ctgatcgcgg tgccgtgcgc gccgcagccg aagcagtgat agaactgctt ggtggggctc
      541 acggtgaacg acggagattt ctcgctgtgg aaggggcagc gggcggcgta gttggatccg
      601 gcccgcttga gcggcacgta ggactcgatc aagtccacga tatccacccg gttgaggagg
      661 tcctggatga aggactgtgg aatcatttcg tcgggatggt ctcgcctcgt ttccccctga
      721 caaccggggt tggtcgccgt cagcggaact cctcggggct ttcgaagaat tataggttac
      781 tttccacgga tttccgggaa agccaccagc gccgaaatct ggcagtttta tattatcttt
      841 taatcacttg cctgatctcc actaccttga ccaggggcga aaccgcagcc agcttggcgc
      901 ttcctgggcc cgcaagcccg tcaagcgacc cgtggcggga ggcggcgcca gggcgccgca
      961 gccttcgatt acgccgataa cttcgccttg accaaggctg atactttgcc catgtcggcg
     1021 cgcccggcca gttgggcttt gagcacggcc atcactttgc ccatgtccgc catgcccctg
     1081 ccgccgctcg tctggatggc cgtggcaacc gccgcctcga ccgccgcatc ggagagtgcc
     1141 tgcggcatgt aggcctgcaa aaccgacagc tcgaattgct cgacatcgac cagatcctgg
     1201 cgcccgccct tctgaaactg ctcgatcgaa tcgcgccgct gtttgatcat cttctcgatg
     1261 accgacatca cgtcggtatc gctcatgtcc ttgcgctcgt ccacctcgcg ctgcttgatc
     1321 gcggcgagca gcaagcgaat ggcggacagg cgctgcgcat ccttcgcgcg catcgcggtt
     1381 ttcatgtcgt cggttatgcg ggcctttaaa gtcatgtgaa ggataaatgg tgaatgcgtg
     1441 aagagtagaa gcgaaaaggg tgaaggtctt acccttcacc cttcactgtt cacccttcgc
     1501 aggattgctc aatagagctt gggcggaagg agttggttac gcaggcgctt gtgatggcgc
     1561 ttgatggccg cagcgagttt gcgtttgcgc tcggcagtgg gcttctcgta gaattcgcgc
     1621 gcccgcagtt cggtcagcag accggttttt tccaccgtgc gcttgaagcg gcgcatggcc
     1681 acttcaaacg gctcgttttc cttgactcga atcgtcggca tagaaagtaa agcagtcctg
     1741 aaagttgtcg gtcaaaacaa aggggcgcat tctagcaata atcccctgat ttgcaaatac
     1801 aaccaggcga ctgaccggcc ttgcggccag cgccgcgcag cgccggcgta cgcctgttgc
     1861 gctcaaccat tcccctataa ctcaatgcct tatgctggtt ctcgggatcg aaacgtcttg
     1921 cgatgaaacc ggggtcgccc tctacgacac cgggaccggg ctgctcggtc acacgctttt
     1981 ctcgcaggtg acgatgcacg ccgagtacgg cggagtggtg ccggaactcg cgtcccgcga
     2041 tcacgtgcgc agacttttgc ctttgacccg gcagctgctg gagcgctgcg gacgccggct
     2101 tgtcgatatc gacgcgatcg cctacaccca gggaccgggg ctggcaggcg cgctgctggt
     2161 gggcgcggcc gtggcagcga gcctgggtta tgcgctcaag gtgccggtgg tcggactgca
     2221 ccatctcgaa ggccacctgc tctcgccgct gctcgccagc ccggcgccgc gttttccttt
     2281 catcgctctg ctggtttccg gcggccacac acagctaatg cgggtggacg ctgtgggcga
     2341 gtaccagttg cttggcgaaa cggtggacga cgccgccggc gaggccttcg acaagactgc
     2401 caagctgctg ggcttgccct accccggggg tcccgcgttg tcgcaattgg cgcagtccgg
     2461 caatcccaag cgtttcaagc tgccgcggcc catgctcgcc agcggcgatc tgaatttcag
     2521 tttctccgga ctgaaaaccg cggtactgac gctggtgcgc ggggccgatc cggacagtcg
     2581 caccaaagcc gacattgccg catcgtttca gcaggcgatc gtcgaagtcc tggtggccaa
     2641 gtcggtcagc gccgtcgccc gggaaggact ggatcaactg gtggtcgctg gcggcgtcgg
     2701 cgccaatcgc ccgttacgcg aggccatgtc aaagaaagcc gaacgcgagg gcttcgaggt
     2761 gttctacccg ccgcaggatc tgtgcaccga caacggggca atgatcgctt tcgccggtgc
     2821 gctgcgtttc gcggccggcg cacctggcgc aatgggcttc acggtacggg cacgctggga
     2881 tttggaagac ctcaagaccg tctcaacagc ctgatcctgc tttcgttgcc tgcgagcaac
     2941 ctttcgatgt tgccgcggtg gcgccatacc agcagaaccg caataaccaa caccgcccag
     3001 gtcatcggcc cccagccgag aaagtggaaa gctgcgagtg gagacagcgc cgccgcgacg
     3061 accgacgcca gcgacgaata gcgcgagaac accgcgacca gcacccacat taccagcagc
     3121 gtgagtgcga tgcgccagtc catcgccagc agaatccccg ccgcagtggc gacccctttg
     3181 ccaccgcgga agcggaaata gagcgggaac aggtgcccga gaaacaccgc gagcgctact
     3241 gcggcaagcg ccgattcgcc gacatcgaac cgtgttgcaa actgctgcgc cagccacagc
     3301 gcgactgcgc ccttcgccgc atcgccgatc agcgtcagca ccgccgcggc tctcttcccg
     3361 gtgcgcaaca cgttggtcgc ccccgggttg cccgagccgt aagtgtgggg atccggcagc
     3421 ccgaagaacc tgctcaccac caccgcgaag gaaatcgagc cgatgagata ggcagccagg
     3481 acgaacagcg cggaggccat ggaattgggt tctccttgag tatcagccgc gggggtgatg
     3541 tttcgcgtgc agctgcttca gccgttcgcg cgcgacgtga gtgtaaatct gcgtggtgga
     3601 aatgtcggcg tggccgagca gcaattgcac cacgcgtagg tcggcgccgt ggttgagcag
     3661 atgggtcgcg aacgcatggc gcagggtgtg cggcgaaacc agcttgctca agcccgcttt
     3721 ggcgacatag cgacggatca gatgccagaa cgcctggcgc gtcatcgaat cgccgcgcgc
     3781 agtgacgaac agtgcatcgg tgcgtttccc gtcaaggatc ttcgggcgcg cctgcgtaac
     3841 atagcggcgg atccacgcca gcgcctcctc acccagcggt accagccgct ctttcgatcc
     3901 cttgccgact atgcgcacca cacccatgtc ctggcttact tgcacaacac ctacggacac
     3961 caattcggag acccgcaacc cgctcgcgta aagcgcctcc agcatcgcct tgtcgcgcag
     4021 gcccagcggc gtgtcaacgt cgggcgcagc gagcaaccgt tcgatatctt cctcggtcag
     4081 gctcttgggc aggctacgcg gcagtttcgg cgcttccacc tgcagcgtcg gatcggccgt
     4141 gatgccgcct tcgcgcaaca ggtattggta aaggcgcttg aggctggaaa gcagtcgcgc
     4201 cgcggagcgc ggcttggcat ggcgctggaa cttgtgggcg agataaccaa gcaggtcgac
     4261 gcgttcggct tccggcaggg tcttgccgcg ctccgatgcc agccacacgg cgaacagccg
     4321 caggtcgcga cggtaacttt ccagcgtgtt gcgcgacagg ccgtcctcga gccagagcgc
     4381 gtcgcagaat tcgtccagaa gaccgtcgtc cgcggccatc agtaggcgcc cggattctcg
     4441 tgatgcagca gccagcgctt gatctcgatc ggcgagccgc ggcgagtgtg catgaagccg
     4501 ccgattccgt cgctggcgat gacgcggtgg cagggaatca ggatcgggat gcggttcgcg
     4561 ccgcaggcgc tgcccaccgg ccgcggcgcc gaatcgatgc gcctggccac ttgcaggtag
     4621 gacagcacct tgccgggcgg gatggcatgc accgtctgcc agaccttgcg ctgatactcc
     4681 gtaccgtcgt attcgaatgg caggtcgaat tcgaaatcgg gatccttgag gtacgcggca
     4741 agttgccggc acacttcttt ggcgaacggc gtctgcggtt tcagcgtcgc cgcgccgcgc
     4801 ggcaggtact cgatgtgggt cagacgctcg ccaatgacgc ggataccgag caccgcgaac
     4861 ggcgactgca tcctggcctg atgagattcg agcatcgggt tcatcggctt gagtgcaccg
     4921 ggggagacgg tgatctgtgt tgggctccgg tattgtagca acgatgccgg ctgcaggccg
     4981 cggcgctact tggctatact ctgggttgca ggtacctatt ccacaaccgg cccaagtgaa
     5041 tactctcgat ccatccaata tctccacttg cagcctggag agcatgcagt tgcgtgtggg
     5101 agaccggctg caactggagc tgcaaaccga cagcacgcgc agccactact acaccacgct
     5161 gatcggctat gtgtccggtc acagcgtgct gatacgcacg ccgcaggtcc agaacctgcc
     5221 gatcccggtg cgcgagggcg agccggtgct ggtgcgaact ttctccggcc gccacgctta
     5281 caccttcgga tccaaggtgg atcgtgtctg ccgcacgccg tatccctacc ttcatcttgc
     5341 ctaccccgca caggtccagc agacgttgat tcgcggagcg ctgcgggtac gggtgaacct
     5401 gaccgccact gcgttgaatg cggcaaatca gggcgaggcc ctgccgtcgg cgttgacgat
     5461 cgccgacctg agcatctcgg gggcgctgct ggaggcggag aagagcctgg gcgaggtggg
     5521 cgagacgctg gagcttgcat tcaagttcct ggtgcagccg aacaattacg aagtgaagct
     5581 ggctacgccg gtggaaatcc agagcgtgcg caagaccagg aggcagcgca gaccggagga
     5641 agtgttcacc catggcgtgc gcttcgccaa gctgcgctcg accgagggtt tgctgctgca
     5701 aagctacgtc cagcaggtgc tgctcgatga ccgctcgcgc gtggtgtgaa gaattcgcgg
     5761 cgcc
//
LOCUS       DASQOP010000157         4137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1472152, whole genome shotgun sequence.
ACCESSION   DASQOP010000157 DASQOP010000000
VERSION     DASQOP010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1472152"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="VE030_06410"
     CDS             complement(<1..898)
                     /locus_tag="VE030_06410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HZF23084.1"
                     /translation="MSSEIVLGWDLGGAHLKAAAVARSGKVVWVRQLPCPLWLGLEHL
                     DRAVASVLEDQPGASRHAVTMTGELTDLFRNRAQGVTGILSCFGRHVPARHTLVYAGE
                     SGFFTPQRAARNTEAIASANWAASAQLVASHVPDALFVDIGSTTTDIVPIRDGKLAVR
                     GHNDYERLACEELVYTGIVRTPVMALAERIPFEGNWIVPMAEYFATTADVYRLTGELP
                     EGTDQLPAADNAGKTGRDSARRLARMLGRDIESAPLAMWRQCARYLAELQLRKIGHAC
                     DRVLSRGEVANRAPLVGAGVGRF"
     gene            complement(888..1880)
                     /locus_tag="VE030_06415"
     CDS             complement(888..1880)
                     /locus_tag="VE030_06415"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HZF23085.1"
                     /translation="MRIFVYEHITGGGMLDDPQVAALAPEGEMMLRALVNDLTAIPGV
                     EVTILRDARLSADLPASIHTVSTAEAFWPAFRRAASEADAVWPIAPEQDGTLARITRE
                     VIDCDRVLLGCRPDAVEITTSKRATAEVLGRGGIPVIPVYAEEDAVPPEVNEIVAKPD
                     DGAGCQDTRLFQDRTELRDWLQAHPDPKRVLQPYLHGEARSLSMLCCDGRARLLACNR
                     QKVETVGGAFRFGGVSVNAISDRDGRYAELASRVARALPGLWGYCGVDFIETVCGPVV
                     VEVNPRLTTSYAGLNRAIGVNPAKLVLGLPASLETSNEPMRESRSIEVELAHVV"
     gene            2140..2841
                     /locus_tag="VE030_06420"
     CDS             2140..2841
                     /locus_tag="VE030_06420"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HZF23086.1"
                     /translation="MIPVVDLLKGQVVHAREGRRAEYRPVQSRLCEGADTETVVDALL
                     RLHPFRSLYVADLDAIQRQGSNVDTLKRIRRCFPHLDLWVDSGIADERGLDAWLRANI
                     GRAVIGSETLVDAEFMSHARQRCRDLSPVLSLDFIAEEFKGPQALLADPARYWPERLL
                     AMNLRRVGSDAGPDLALIATLAAKAPACEVYAAGGVRCVEDLERAAAAGAAGALIATA
                     LHDGRLGPSHLAQFT"
     gene            complement(3131..3643)
                     /gene="fae"
                     /locus_tag="VE030_06425"
     CDS             complement(3131..3643)
                     /gene="fae"
                     /locus_tag="VE030_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016840 - carbon-nitrogen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-activating enzyme"
                     /protein_id="HZF23087.1"
                     /translation="MAKINRVCVGESLVGEGNEVAHIDLLIGPRGSAVESAFCNALTN
                     NKDGFTSLLAVVAPNLITKPNTVMFNKVTIKGAKQAVQMFGPAQRAVAMAVADSVAEG
                     VIPMAEADDLFLCVGVFIHWQAADDKKIQDYNYKATKEAIARAVNGDPKAKDVVAKKG
                     TVDHPFQAHK"
     gene            complement(3716..>4137)
                     /locus_tag="VE030_06430"
     CDS             complement(3716..>4137)
                     /locus_tag="VE030_06430"
                     /inference="COORDINATES: protein motif:HMM:NF013991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HZF23088.1"
                     /translation="ERDLIARQYVTDYRDVFDTGMPALRGALARWARPEWAAVAVYLE
                     FLANFPDSHVAGKHGGEIAAAVSGEAGLLVQALGRASDPAQEMPALEAFDRQLKARSI
                     NPGTSADMTVATLVAMDLEDSLVRRFHECSAEVAAGE"
BASE COUNT          755 a         1357 c         1237 g          788 t
ORIGIN      
        1 ggaatctgcc gacgcccgcc cccaccagcg gcgcacggtt ggctacttcg ccacgcgaaa
       61 gaacgcggtc gcatgcgtgg ccaatcttcc gtagctgcaa ctccgccaga tagcgcgcgc
      121 attgccgcca catggcaagc ggtgcggatt cgatatcacg ccctagcatt cgcgcaagcc
      181 gccgcgcgct gtcgcgcccc gtcttgccgg cgttgtcagc ggcgggaagc tggtccgtgc
      241 cctcgggcaa ctcgccggtc aggcgataga cgtcagcagt ggtggcaaag tattccgcca
      301 tcggtacgat ccagtttcct tcgaaaggaa tgcgctccgc cagcgccatg accggtgtgc
      361 gcacgatgcc ggtatagacc agttcctcgc aggcgagacg ttcgtagtcg ttgtggccac
      421 gcactgcgag cttgccgtct cggatcggga caatgtcggt ggtcgtgctg ccgatgtcca
      481 cgaacagcgc gtccggcaca tgactcgcca ccagttgcgc gctggctgcc cagttggcgg
      541 aggcgatcgc ttcagtattc cttgcagcac gctgcggcgt gaagaatccg gattctcctg
      601 cataaaccag agtgtgccgt gctggtacgt gtctgccgaa gcaggacagg atgccggtca
      661 caccttgcgc ccgattcctg aagagatcgg tcagttcgcc ggtcattgtg accgcatgcc
      721 gactggcgcc gggctggtct tcgagcacgc tcgccaccgc gcgatcgaga tgctccagcc
      781 ccagccacag cgggcacggc aactggcgca cccacacaac ctttccactg cgagcaaccg
      841 ccgcggcctt gagatgcgcg ccacccaggt cccagccgag aacgatctca gacgacatgg
      901 gcaagctcga cctcgattga gcgtgattcg cgcattggtt cgttcgaggt ttcaagcgac
      961 gcgggcaggc cgagcacgag tttggcgggg ttcacaccaa tcgcgcggtt cagacctgca
     1021 taggaagtgg tgagccgcgg attcacttcg accaccaccg ggccgcaaac agtctcgatg
     1081 aaatcgaccc cgcagtaacc ccacaggccg ggcaacgcgc gcgcgactcg cgacgccagt
     1141 tcggcgtaac gaccgtcgcg atccgaaatc gcgttgacac tgacaccgcc gaatcggaat
     1201 gcgccgccga cggtctcgac cttctgccga ttgcaggcca gaagccgggc gcgtccgtca
     1261 caacaaagca tggacaggct cctcgcctcc ccgtgcaaat agggctggag tacccgcttt
     1321 ggatccggat gggcttgcag ccagtcacgt agttcggtgc gatcctggaa aagccgggtg
     1381 tcctggcagc ctgcgccgtc gtcgggcttc gccacgatct cgttcacttc cgggggaaca
     1441 gcgtcctcct cggcatagac cggaatcacg ggaattccgc cacggccaag cacttccgcg
     1501 gtggcacgct tgctggtcgt gatctcaact gcatcggggc ggcagccgag cagcacgcgg
     1561 tcacaatcaa tcacttcccg cgtgatgcgg gcgagggtgc catcctgctc cggcgcaatc
     1621 ggccacacgg catcggcttc gcttgcggcg cgacggaacg caggccagaa cgcctcggcc
     1681 gtactcaccg tatggatgga cgccggcaga tcggctgaca accttgcgtc gcgaaggatt
     1741 gtaacttcca cgccgggaat cgccgtgaga tcattcacca gcgcgcgaag catcatctcg
     1801 ccttccggcg ccagcgcggc gacctgcgga tcgtccagca tcccgccgcc ggttatgtgc
     1861 tcataaacaa aaatccgcat gggaaggaca aactctttca acacgaagga tcacgaagga
     1921 aaaatacaag gcacacgagg gagaagcaaa atccaataga attgaatgtc actcatatgg
     1981 gggatggccg ccctcaaagt aagtaacagt ctgcatgact ttacgggatg gcgacatacc
     2041 atcctgtggt tcattgtctt tccttcgtgt accttcgcct ttccttcgtg acccttcgtg
     2101 ttaaccgcgt ttcgcccttc ctctgtggct aagttgaact tgatccctgt cgttgacctg
     2161 ctgaaagggc aggtggtgca tgcgcgcgaa ggtcggcgcg ccgagtaccg gccggtgcag
     2221 tcgcgccttt gcgaaggcgc ggacaccgaa acggtcgtgg atgcgctgtt gcggctgcac
     2281 cccttccgtt cgctctatgt cgccgacctg gatgcgatcc agcgccaggg cagcaatgtc
     2341 gataccctca agcgaatccg ccgctgcttc ccgcatctcg atctgtgggt ggactcgggg
     2401 atcgcggacg aacgcggcct cgatgcgtgg cttcgcgcca acatcggccg ggcggtaatt
     2461 ggcagcgaaa cccttgtcga tgcggaattc atgtcccatg ccagacaacg ctgcagagac
     2521 ctgtcgccgg tgctttcact ggacttcatc gccgaggagt tcaaaggccc gcaagcgctg
     2581 ctcgccgacc cggcacgcta ctggccggag cgcctgctgg cgatgaacct ccggcgggtc
     2641 ggcagcgatg cagggcccga ccttgcgctg atcgcgaccc ttgccgcaaa ggcgccggcc
     2701 tgcgaggtgt acgcggcggg cggcgtccgc tgcgtcgaag atctcgaacg cgctgccgcg
     2761 gcaggcgccg cgggtgcgtt gatcgcgacc gctcttcacg acggccgcct cggcccctcg
     2821 cacctcgcgc aatttacgta agaccaccac tcaccacgga ggacgcggag gacacggagg
     2881 aaaggcaaga gcaagaaaac atgctcgggt caaaaggctt tcattggttg atgacccatc
     2941 gccgtgaaag ttgacggcac aggttcgcgg acagtcaccc cgcttttaac tccagtttgc
     3001 ttttcctagt ttttcttttc ctccgtgtcc tccgcgtcct ccgtggtgag cgctgccctt
     3061 cttaagagag aaaccgtgaa aaagcccgct ccgggaaagc ggagcgggcc tggttcgatt
     3121 tcgtgccgga ttacttgtgg gcctggaagg ggtggtccac ggttcctttc ttggcgacca
     3181 cgtccttggc tttcgggtcg ccgttgaccg cgcgcgcgat tgcttccttg gtggccttgt
     3241 agttgtagtc ctggatcttc ttatcgtcgg ccgcctgcca gtggatgaac acgccgacgc
     3301 agaggaacag gtcgtccgcc tcggccatgg gaatcacgcc ttcggcgacg ctgtcggcaa
     3361 ccgccattgc cactgcgcgc tgggccggac cgaacatctg caccgcctgc ttggcgccct
     3421 tgatcgtcac cttgttgaac atcacggtat tcggcttggt tatcaggttc ggcgccacca
     3481 ccgcgagcag cgaggtgaaa ccgtccttgt tgttggtcaa tgcgttgcag aacgccgatt
     3541 ccaccgcact gccgcgcggc ccgatcagca agtcgatgtg ggcgacttcg ttgccctccc
     3601 cgaccagcga ctcgcccacg catacacgat ttatcttcgc catcttgttc ctccttctca
     3661 ggtttcttga agttggttgc tgaacacggg ccgagacccg ccactcactg cgagctcatt
     3721 cgccggcggc gacttccgcg ctgcactcgt gaaaacgcct caccaacgaa tcttccaggt
     3781 ccatcgcgac cagggtggcc accgtcatgt cggcgctggt acccgggttg atggaccttg
     3841 ccttgagctg gcggtcgaac gcctccagcg cgggcatttc ctgcgccggg tcgctcgcac
     3901 gccccagcgc ctgcactaac aaacctgctt caccgctcac tgccgccgcg atctccccgc
     3961 cgtgcttgcc cgcgacgtga ctgtcgggaa agttcgcaag gaactccaaa tacacggcca
     4021 ctgcggccca ctcgggacgg gcccagcgcg ccaacgctcc gcgcagcgca ggcatgccgg
     4081 tatcgaacac atcgcggtaa tccgtgacgt actggcgagc gatgagatcc cgctcgc
//
LOCUS       DASQOP010000158        13967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_870622, whole genome shotgun sequence.
ACCESSION   DASQOP010000158 DASQOP010000000
VERSION     DASQOP010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13967
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_870622"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VE030_06435"
     CDS             complement(<1..570)
                     /locus_tag="VE030_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23089.1"
                     /translation="MIFHMPHLTNADIVVGERIRMRSFASRHLTRALLLGLLVAFSLA
                     WPALAAEKRTKPKQNGVESPGMYSNYVDKGQSAVDQYLGDTRALTEAELLPALLSAID
                     QLSKYPRPTFVPEIFRVPHERLQELACTSKCGVLATYQPGEGIFLDERLKPETNLFDR
                     SVLLHELVHYVQELNHERGDARPCERWYYR"
     gene            complement(791..1657)
                     /locus_tag="VE030_06440"
     CDS             complement(791..1657)
                     /locus_tag="VE030_06440"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZF23090.1"
                     /translation="MDSRAVLTAALISFSVCAFALDQEETFINLTPGQDMLDPANLAQ
                     LLRQGDVRAMNNVGLLWAKGYNGKQSYIEALNWWKEAAKRGYTVAMNNIGLMYAKGHG
                     VEQDFKEAFAWWHQAAFLGNAWAMNNVGDLYENGHGVEQNLTMAMTWYQSAAEQGERM
                     AMYNIGSLYEAGKGVDRDYAAAMTWFRKAADKGDASAMHSIGAMYAEGRGVGPDPVEA
                     HAWYSVAGLRYPAEDADEAKRNRHDLDELGARLDPEQLARAKDRAAALEALTRPVAPE
                     PPKPLAPGEKAT"
     gene            1855..2712
                     /locus_tag="VE030_06445"
     CDS             1855..2712
                     /locus_tag="VE030_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011494342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZF23091.1"
                     /translation="MSVVSFAFDVLAYSAIFILVVMGLGVIASMMGIFNFAHGEFVLL
                     GAYTVYLFQERGLPVWAGMLAAPFAVAVVGLLLERTVIRRFYAAPVAAMLGTYAIGLS
                     LREGVRGLLAGQFYSVISPLVGSFDLGDISVSKWKAAVILMTLLVMVGSWLLLTRTRF
                     GLQVRATLENPALARASGISTDKIYAATFSFGAALAGLAGGLMVPIYSLNADMGVPFL
                     IKSFLAVMLGGAGGFEGSMAGAAFVGASSAALPWVIKPVVADVLVFVIAIAIVKFRPE
                     GLLSRWRSS"
     gene            2713..3942
                     /locus_tag="VE030_06450"
     CDS             2713..3942
                     /locus_tag="VE030_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011494341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding protein"
                     /protein_id="HZF23092.1"
                     /translation="MDQDDYKPRLQSYSRRLFVQRAGLLSGMVALGGGSYLLNPTGAW
                     AASPIKVGIATDLTGPISWGGIPNSNVAKMVIQDINASGGLLGRPLQMILEDTATNEA
                     LAVTKVRKLVQKDKVDVVFGGITSSMRNAIKDTIVNRGKTLYIYPQLYEGKECTQYLY
                     CTGPTPAQQCDTFIPWLIKNGGRKFYLPSADYVWPHVLNAYARNVIEKNGGEVVGEEY
                     FPVDHNEYSATVQKIMSNKVNVVFNTTIPPGVAAFFKQLYEAGFQKNGGRLACVYYDE
                     NALNITPAHEIEGLASCLDYFKAVNDPYGNKLQEKYSKLFGDKNPFTAGSAATGMYRG
                     IRLWAEAVRTAKSVKREPVAAALDHARIADGPGGGSEVVPGTRHLKMNMYTAVAKSGK
                     YEIVEKSNGPVMPGECA"
     gene            4192..5196
                     /locus_tag="VE030_06455"
     CDS             4192..5196
                     /locus_tag="VE030_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011494340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZF23093.1"
                     /translation="MIPWVEGTVLVLFFLLPVVLDDFWVLFFTRVLILGLLALSFDLV
                     WGYAGILSLGQALFFGMAGYSCALLATELGITSILILLPTAALVGLTVALVLAAMVIL
                     GKRVPTTVFVALGTLTGSYVVERLARGWSYVGGQNGIPSLPRLTLGQHEITEGRVYYY
                     LALAILTAIYLALRWLVRSQFGLVLAGIREQETRMPFLGYKVQNYKGVVFALAGLIAG
                     VSGGLYAFHEGYVGPGQLGPVLSTQVVLYVLFGGVGTLIGAIIGVLTIDLFSFHLSEQ
                     WETGWPIVLGLLLLAVVTLRPTGVIGIFVSERERVGSFGKPDLSDSRAEVSGNEPGKP
                     "
     gene            5193..5927
                     /locus_tag="VE030_06460"
     CDS             5193..5927
                     /locus_tag="VE030_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011494339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZF23094.1"
                     /translation="MILLRVQGVRKVYATLTALDGVDLTVMKNSFHGLIGPNGSGKST
                     LLKCIAGAEFANQGSVTFAGRDITRATPAARSRAGMSLKFQITSVLPSLSVYDNILLA
                     LQAQEVMGTLALSRSRGRLHGRVIDLLAQFSLFEKRHDLAGSLSHGQQQWLEIAMAIS
                     RDPALLLLDEPTAGMSRQERHITGELLIPIKQRCSVLIVEHDVDFIKDICDTLTVLDQ
                     GTVVESGTTQHVQNSERVREAYLTHV"
     gene            5920..6624
                     /locus_tag="VE030_06465"
     CDS             5920..6624
                     /locus_tag="VE030_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011494338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZF23095.1"
                     /translation="MSEANLLEASGLCTHYGTSQALFDVGLAVPARGGVAVLGRNGAG
                     KTTLLKTLAGELKPSRGRVYFDGVDAMRLSTEQRIRRGIGYVPQEQGTFSRLTVKENL
                     LIGAMGAPGGDRRIDEVVELFPQLGQRLRQQAGTLSGGERKMLAIGRALLGRPHLLML
                     DEPTEGVWHGVIEEIAERLERLAKEIALVLVEQNIRLALRICRYCYVMDRGHIGLEGP
                     AGRIGDDPELVRLLAP"
     gene            6697..8211
                     /locus_tag="VE030_06470"
     CDS             6697..8211
                     /locus_tag="VE030_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZF23096.1"
                     /translation="MAVQIPTPQQVREAAAEVGLSLTDADVQSYIGLMKGNIAAYNLV
                     DAMPDNLPAVKYPRTPGYFPPPEENKHNAWYVKTSIKGAESGPLRGRRVAVKDNVMVA
                     GVPMMNGCSIMEGYVPEVDATVVARLLDAGAEIVGKTHCESYCISGGSHTGSKGPVHN
                     PYKMGYSAGGSSAGSGVVVALGEADMALGCDQGGSIRMPSGWCGIYGMKPTHGLVPYT
                     GIMPIEIFVDHVGPMTRTVKDNALMLEVIAGPDGYDPRQYAPKVHKYSQMLDAGVKGM
                     KIAVVKEGFGRPESESTVDEKVKKGAELFRKLGATVDEVSIPMHLMGPVIWTPIGVEG
                     IAQTMMFGDGYAQSRPDLYIVSLMDKLHGWTARAGELSETTKILTTLGVYIKKYHGSR
                     YYGKAMNVTRRLVEAYDKVLEKYSLLLMPTLPMKPTPLPPPDANRELYVQRALEMIGN
                     TCPFDITHHPAMTLPCGMIDGLPVGLMLVGKHYDEPTIYRAAQAFEQTGDWKKM"
     gene            complement(8250..9101)
                     /locus_tag="VE030_06475"
     CDS             complement(8250..9101)
                     /locus_tag="VE030_06475"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZF23097.1"
                     /translation="MQQQTIRANGVRINAWVGGKGDPVVLLHGYPQTGQMWRKVVPTL
                     LDQFAVVCPDLRGYGDSDKPRDGYDKRSMAKDIREVMSALGFESYGLVGHDRGARVAH
                     RLAMDYPGEVKNLAVLDIVPTHTVFRDTGKELAAAYWHWFFFQVPDLPEIMLAASAEP
                     FLRHMFRALCFRQDSIEEPMFQEYLRAFRLPGTIRAGLEDYRAAATVDIADDERDLGK
                     KLACPVLAIWGEYGKMHTLFDVLGTWKEKAANVRGHPVPCGHFIPEEAPDELLGDLLP
                     FLRKGNK"
     gene            complement(9268..10464)
                     /locus_tag="VE030_06480"
     CDS             complement(9268..10464)
                     /locus_tag="VE030_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020693826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HZF23098.1"
                     /translation="MNRPEKLPRNASPHIASRMGGIEPFHVMALIARAKELEAQGRSI
                     SNMVVGEPDAPTPPLIAEAGVRAIQAGRVGYTQSLGITELRDAISEWYRTRYGVDVPR
                     SRIAVTTGSSGALLLTMGAILSPGERVLMTDPGYPCNRHFVRTMEGDPVGVPVDASTA
                     YQLTAKLVEKHWTPKTAAVLIASPSNPTGTLIAHDELQRIHAVVRARGGVLIVDEIYH
                     GLTYGQRDRSALEFADDVFIINSFSKYFGMTGWRLGWMVVPEAIMPAVDKLAQNLFIS
                     SSEVAQKAALAAFHPDTTALLDQRRDAFRAQRDFLVPELRSLGFDIPVMPEGAFYIYA
                     NCERLVSDSYQFCRDVLEKAGVAIAPGIDFGEYRAKQHVRFSYPKSIPVLAEGVERLR
                     KYLGSK"
     gene            10639..11574
                     /locus_tag="VE030_06485"
     CDS             10639..11574
                     /locus_tag="VE030_06485"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HZF23099.1"
                     /translation="MKIAIIGSGGVGGYFGARLAGSGQDVTFVARGAHLEALQKNGLK
                     VLSALGDLHLPEVKATKDTTVIGPVDVVMIAVKLWGTEEAAQSAKPLIGPNTVAVSFQ
                     NGVVAVDTLLPILGKEHVMGGVANIAALIEAPGVIRHNGNMANLFFGELDGKPSPRSQ
                     ALLVACKIANIQAELVTDINGAIWEKFVRLVTLSALTSLTRMPVGPIRSDPDTRVLMQ
                     QVMEEVVAIGKARGVKFDTGIIAEQMARVDGYPATMVASMCGDLRRGNRLELPWLSGT
                     VAKLGEELGIPTPANHFVYAALKLYANGRPADAQV"
     gene            11777..12865
                     /locus_tag="VE030_06490"
     CDS             11777..12865
                     /locus_tag="VE030_06490"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HZF23100.1"
                     /translation="MKLKIKNIRARAVMVPLKRPPVSASGALPEAALVLVDVETGEGI
                     TGHSYLAAYGKPMLKPLVSTTEAVAEMIKGDALVPYEIDAKLRKRFTLFDTPGLLGLA
                     LAGIDMAAWDAYTKGLGVPLVCALGGQIKPVRAYNSCGLWIKDPALLADEAAELLAEG
                     GYSAVKLRIGRPNFKDDLTAVRNVKKRIGGDISLMTDFNQSQTVTEAILRGRALDDEG
                     LYWIEEPVRHDNYAGCAKIALEVNTPIQIGENLLNTFEMQKAIEAKAAEYYMPDVQRI
                     GGVTGWMRAAALAHAHHLEMSSHLFPEISAHLLAVTPTCHWLEYMDWANPILREPFEV
                     KNGNVIIPDRPGAGIAWNEDAVQRYAPA"
     gene            13387..13656
                     /locus_tag="VE030_06495"
     CDS             13387..13656
                     /locus_tag="VE030_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23101.1"
                     /translation="MMRLGFLLALPLVLASLSFAADDSKKTRVGTCTDAKSQMEYFCN
                     PANAASDSMVAIGTACTNARNNVKEACEGIVEPDKKYEFDDKKKE"
     gene            13719..>13967
                     /locus_tag="VE030_06500"
     CDS             13719..>13967
                     /locus_tag="VE030_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23102.1"
                     /translation="MKAHSLITALVLGLAATVAFAAEKDTRGGTCDDAKKQHEYYCEG
                     KNDPNDIFTQANIACNNAKRNMAAACEGKVAPDAEYKFE"
BASE COUNT         2807 a         4105 c         4255 g         2800 t
ORIGIN      
        1 gcggtagtac cagcgctcgc agggcctggc gtctccgcgt tcgtggttga gctcctgcac
       61 gtaatgcacc agttcgtgca acagcacgga acgatcgaac aggttggttt ccggtttgag
      121 gcgctcgtcc agaaaaatcc cctcgcccgg ctggtacgtg gccagcacgc cgcacttgga
      181 ggtacaagcc aattcctgca gcctctcgtg cggaacgcga aagatttccg gtacgaaagt
      241 cggccgcggg tatttggaca actggtcgat cgcggacagc aaggccggta gcaattcggc
      301 ttcggtgagc gcacgggtgt cgcccagata ctggtcgacc gctgactgac ctttatccac
      361 ataattgctg tacatgcctg gggactcgac gccgttctgc tttggcttcg tgcgtttttc
      421 ggcggcgagg gcgggccacg caaggctgaa cgcgaccagg agtcccagca acaaagcccg
      481 ggtcagatga cgagaagcga acgatcgcat gcgaatcctt tccccgacca cgatatcagc
      541 gttggtgagg tgcggcatgt gaaagatcac gaaccccggt taccacgaag gcacgaagac
      601 acgaaggaaa agcaaaacga atgaagtgag atgcagaata ttgcgcggtt cgagacaaac
      661 ttcgtaaaag tttgcctacc gtaagtaggg acatcgaact cccttccgct cttcttccct
      721 gttcttcgta ttgcgtacct gattttcctt cgtgacttcg tgccttcgtg gtacgactgt
      781 attcagaatc tcaggtggcc ttctcgccgg gggcgagagg tttcggtggc tcaggtgcaa
      841 ctggacgggt cagcgcctcg agggcggccg ctctgtcctt tgcgcgcgca agctgttccg
      901 gatcgagccg tgcgccgagt tcgtcgaggt cgtgccggtt ccgcttcgct tcgtcggcat
      961 cctcggccgg ataacgcaag ccggcaacgc tataccacgc gtgcgcttcc acgggatcgg
     1021 gaccgacgcc acgcccttcc gcatacatcg cgccgatgct gtgcatcgcc gaagcgtcac
     1081 ccttgtccgc agccttgcgg aaccaggtca ttgccgcggc ataatcgcga tcgacgccct
     1141 tgcctgcttc gtagagcgag ccgatgttgt acatcgccat gcgctcgccc tgctcggcgg
     1201 cgctctggta ccaggtcatc gccatggtca ggttctgctc gacgccgtga ccgttctcgt
     1261 agaggtcgcc gacgttgttc attgcccagg cgttgccgag aaacgccgcc tggtgccacc
     1321 acgcgaacgc ctccttgaag tcctgctcca cgccgtggcc cttggcatac atcagcccga
     1381 tgttgttcat cgccacggta tagccgcgct tggccgcctc cttccaccag ttgagcgctt
     1441 caatgtagct ttgcttgccg ttgtagcctt tggcccagag caggccgacg ttgttcatcg
     1501 cgcggacgtc gccctggcgc agcagctgcg caaggttggc cggatcgagc atgtcctggc
     1561 cgggcgtgag attgatgaag gtctcttcct ggtccagcgc aaaagcgcaa acggaaaatg
     1621 aaatcagggc tgcagtcagg acggcgcgtg aatccacaaa tttttctcca agcctgaaca
     1681 agtaacggac ccttacctgc agtgcatgat gccactccat gccgcgatgc gtaaagccgc
     1741 aagtccctgc tttgggcttg atttgtccaa ttgcaagcgc gccggacaaa gtgctagcat
     1801 ccgcgaacgc tttgtgcgtt cctctggcgc gattctccct tctctgggtg gtcaatgagc
     1861 gtcgtatctt ttgccttcga cgtgctggcc tacagcgcca ttttcatcct ggttgtgatg
     1921 ggcttgggcg tgatcgcgag catgatgggc attttcaatt tcgcccatgg cgaattcgtg
     1981 ctccttggcg cgtacaccgt ctacctgttt caagagcgcg ggttgccggt ttgggcgggg
     2041 atgctcgcgg cgccatttgc tgtagcggtc gtcggcctgc ttcttgagcg tacggtgatt
     2101 cgccgcttct atgccgcgcc tgtagctgca atgctgggca cgtacgcgat cggtctgtcg
     2161 ttgcgagagg gcgtgcgcgg tctgcttgca ggtcagttct actcggtaat ctcacccctt
     2221 gtgggctcgt tcgacctggg cgatatctcg gtctccaagt ggaaggccgc cgtcattctg
     2281 atgacgctgc tggtcatggt gggcagctgg ttgctactca cccgcaccag gttcggcctt
     2341 caagtgcgcg cgacgctcga gaaccccgcc ttggcccgcg cttcgggcat ctccacggac
     2401 aagatatacg ctgccacatt cagcttcgga gctgctctgg ccggactggc gggcggactc
     2461 atggtgccga tatattcgtt gaatgccgac atgggggttc cgttcctgat caaatccttc
     2521 ctcgccgtca tgcttggcgg cgcaggcggg ttcgagggtt ccatggcagg ggctgcgttt
     2581 gtcggtgcaa gcagcgcggc gctgccttgg gttatcaagc cagtagttgc agacgtactg
     2641 gtcttcgtga tcgcaatcgc gatcgtgaag tttcggccgg aaggcctgtt atctcggtgg
     2701 aggagttcct aaatggatca agatgattac aaaccaaggc tccaatccta ttcgcgtcgt
     2761 ctattcgtgc agcgcgccgg cctcctttcc ggaatggtcg cgctcggcgg cggcagttac
     2821 ctgctgaatc ccacgggtgc atgggcagcc agtcctataa aagtcgggat agcaaccgat
     2881 ctcaccgggc cgatcagctg gggaggcatt cccaactcga acgtggcaaa gatggtaatt
     2941 caagacatca atgcgagcgg cggcctgttg ggacggccgt tacagatgat tctcgaggac
     3001 accgccacta acgaggcact ggccgtcaca aaggtccgca agctggtgca aaaggacaaa
     3061 gtggatgttg ttttcggcgg aatcaccagt tccatgcgaa acgccatcaa ggacactatc
     3121 gtcaaccgcg gcaaaacgct ctatatctat ccgcaattgt atgaaggcaa ggaatgcacg
     3181 caatacttgt actgcaccgg ccccacgccg gcacaacagt gcgacacctt cattccgtgg
     3241 ctgatcaaga acgggggcag gaagttctat ctcccttccg ccgactacgt ctggccgcac
     3301 gtgctgaacg catacgcgcg caacgtcatc gagaagaatg gcggcgaagt cgtcggcgag
     3361 gaatactttc cggtggatca caacgaatac agcgccaccg tgcagaagat catgtcgaac
     3421 aaggtcaatg tggttttcaa cacgaccatc ccaccgggcg tcgcggcctt cttcaagcag
     3481 ctttacgagg caggattcca gaaaaacggc ggtcgcctgg cgtgcgttta ttacgacgaa
     3541 aacgcgctga atatcacccc ggcccatgag atcgagggac tcgccagttg cctggactac
     3601 ttcaaggcag taaacgaccc ttacggcaac aagctgcagg aaaagtacag caagcttttt
     3661 ggcgacaaga atcccttcac cgcaggcagc gcggcaactg gaatgtaccg cggcatccgc
     3721 ctctgggctg aagcggtcag gacggcgaaa agcgtgaagc gcgaaccggt cgccgccgcg
     3781 ctcgatcacg ccaggatcgc cgatggtccc ggtggtggca gcgaggtagt cccgggaaca
     3841 cggcacttga agatgaacat gtatacggcc gtggcaaaga gcgggaaata cgaaatcgtc
     3901 gaaaaaagca atggtccggt catgccgggc gagtgcgctt gatttccgtg ccgggcaatg
     3961 cctgagacgc accctccccc ggatgtgccg ggggaggtct tcgtcgacgt gccggaacgc
     4021 gcttgccggc atcgaagatt tcattcgtgg acttgagcgc tattgcaatc gcatcataga
     4081 cggtcgggtc gcgcgccagg gatgggactt tgcatttctg gcccgacaac cgttagccct
     4141 tccggatcca tgacgagatc gattttcttc gccaaaaata agagccgacg gatgatccca
     4201 tgggtcgaag gcacggttct ggtcttgttt tttctgcttc cggtggtgtt ggacgacttc
     4261 tgggtgttgt tcttcacccg cgttcttatt ctggggctgc tcgccttgtc gttcgatctg
     4321 gtgtggggtt atgccggtat cttgtcgcta ggtcaggctc tttttttcgg aatggccgga
     4381 tatagctgcg cgttgctcgc taccgaactg ggcatcactt cgattctgat cctgctgccc
     4441 accgccgcac tcgtgggatt gactgtggcc ctggttcttg ccgccatggt gattcttggc
     4501 aagcgcgttc cgaccaccgt cttcgtggct ctgggcacgc tgaccgggtc gtacgtcgtg
     4561 gagcgactcg cacgcggctg gtcctacgtg ggaggtcaaa acggcattcc ttcgttgccg
     4621 cgtttgacgc tcggccagca cgaaatcacc gaaggtcgcg tctattacta cctggcgctc
     4681 gcaattctga ctgcgattta cctggcgctt cgctggctgg tgcgttcgca gttcgggctg
     4741 gtgctcgcgg ggataaggga acaggaaaca cgaatgcctt ttctcggcta caaggtgcag
     4801 aactacaagg gggtcgtgtt cgcgcttgcc ggtctgatcg ccggcgtcag cgggggctta
     4861 tacgcatttc acgaaggtta tgtcggtcca ggccaactcg gccccgtgct gtccacccag
     4921 gtcgtactct acgttctgtt cggaggcgtc ggtaccctca tcggtgccat catcggtgtg
     4981 ctgaccatcg acttgttcag ttttcacctc tccgagcagt gggagaccgg ctggccgatc
     5041 gtcctggggc tgttgttgct ggcggtggtt acattgcgcc ccacgggtgt catcgggata
     5101 tttgtgtcgg aacgcgagcg ggtggggtct tttggaaaac ctgatctgag cgattcgaga
     5161 gccgaggttt caggcaacga accgggaaaa ccatgatctt gctccgcgta cagggcgtca
     5221 gaaaggtata cgccacgctc accgcattgg atggggtcga tctgacggta atgaagaatt
     5281 ccttccacgg cctgatcggc ccgaacggat ccggcaaaag cacgcttctg aaatgcattg
     5341 ccggtgccga gttcgcgaat caggggtcgg taacatttgc aggccgagac atcacccgtg
     5401 cgactcctgc ggcgcggtcc cgcgccggta tgagcctgaa attccaaatt accagcgtgc
     5461 tgcccagcct gagcgtgtac gacaacattc tgctggcgct tcaagctcaa gaggtcatgg
     5521 gaactctcgc attatcccgg tcgcgcggcc gattgcacgg tcgcgtgatc gatctgctcg
     5581 cgcagttcag ccttttcgaa aagcggcacg atcttgcggg ttcgctttcg cacggccagc
     5641 agcaatggct ggaaatcgcc atggcgattt cgcgcgatcc ggccctgctg cttctggacg
     5701 aacccactgc cggcatgagc cggcaggagc gccatatcac cggtgagctg ctgattccga
     5761 tcaagcaacg ctgctccgtt ctcatcgtcg aacacgacgt ggatttcatc aaggatatct
     5821 gcgacacctt gacggtgctc gatcaaggta cggttgtcga gagtggcacc acgcaacacg
     5881 tccagaacag cgaacgggta agggaggctt acctcaccca tgtctgaggc gaaccttctg
     5941 gaagctagcg ggctttgtac tcactacggt accagccagg cattgttcga cgtgggtctt
     6001 gccgtgcccg caaggggcgg cgtcgccgtg ctgggccgta atggtgcggg caagaccaca
     6061 ctactcaaga ctctggcggg cgaactcaag ccctcgcgcg ggcgcgtgta ttttgacggc
     6121 gtagacgcca tgcgcctatc caccgagcag cgcattcggc gcggaatcgg ttacgtacca
     6181 caggagcaag ggacgttttc ccggctgacc gtgaaggaaa atctgcttat cggcgccatg
     6241 ggtgcccccg ggggcgaccg ccggatcgac gaagtggtcg agctgtttcc tcaactggga
     6301 caacggctcc ggcagcaggc tggcacgctg tcgggaggcg agcgcaaaat gctggcgatc
     6361 ggccgcgcgc tactcggcag accacatctg ctcatgctgg atgagcctac ggagggcgta
     6421 tggcacggtg tcatcgagga gattgccgaa cggctcgagc gcttggcaaa agagatcgcg
     6481 ttggtgctag tggagcagaa catcagactg gcattgcgta tctgccgcta ttgttacgtg
     6541 atggatcggg gccacatcgg actcgagggg cctgcaggtc ggatcgggga tgatccggaa
     6601 ctggtgcggt tgctggcgcc atagattccc gggcaaacac aagcacccgg caattgcaat
     6661 ccttgtgctg tttctcacga caaaaggaga ctgaaaatgg ccgtacaaat acctacaccg
     6721 caacaggtcc gggaggccgc cgcggaagtc ggactctcgc tcaccgacgc ggatgttcaa
     6781 tcgtacatcg gtctgatgaa aggcaacatc gctgcctaca atcttgtaga cgccatgccc
     6841 gacaaccttc cggccgtgaa atatccgcgc acgccgggat actttccccc gccggaggaa
     6901 aacaaacaca acgcgtggta cgtgaaaacg tcgatcaagg gagcagagtc cggtccgctt
     6961 cggggaagga gagtggcagt caaggacaac gtgatggtgg ccggggtccc gatgatgaac
     7021 gggtgctcga tcatggaggg ctatgttccg gaagtcgatg cgaccgtggt agcccggctt
     7081 ctcgacgcgg gcgccgagat cgtgggcaaa acgcattgcg agtcctattg catttcgggt
     7141 ggcagccata ccgggtcaaa aggcccggtg cacaatcctt acaagatggg ttactcggcc
     7201 ggcggctcat cggccggaag cggcgtagtg gtggcgctcg gcgaagccga catggcgctc
     7261 ggctgcgacc agggcggttc gatccgcatg ccctccggat ggtgcggcat ctacggcatg
     7321 aagcccacgc acggcctggt tccatacaca ggcatcatgc ccatcgaaat ctttgtggac
     7381 catgtcggcc cgatgacgcg caccgtgaag gacaatgcgc tgatgctgga agtcattgcc
     7441 ggccccgacg gctacgaccc caggcaatac gcgccgaaag tgcacaagta ttcgcagatg
     7501 ctcgatgccg gcgtcaaagg catgaagatc gcggtcgtca aggaaggttt cggtcgcccc
     7561 gaatccgaat cgacggtgga cgagaaggtg aagaaggggg ccgaactatt caggaagctc
     7621 ggcgccacgg tcgacgaagt atcgatcccg atgcacctga tgggcccggt gatttggact
     7681 ccgattggcg tagaaggcat cgcccagaca atgatgttcg gcgacggtta tgcgcaatcc
     7741 cggcccgatc tttacattgt cagtctgatg gacaaacttc acggatggac cgcacgcgcc
     7801 ggcgaacttt ccgagacaac gaaaatcctt acgacgctgg gggtttacat caagaaatat
     7861 catggcagcc gttactacgg caaagcgatg aacgtcacgc gccgcctggt cgaggcctat
     7921 gacaaggtac tcgagaaata cagccttttg ttgatgccga cactaccgat gaaacccacg
     7981 ccgttaccac cgccggatgc aaaccgtgag ctctatgtgc aacgcgcgtt ggagatgatc
     8041 ggcaatacct gccccttcga tattacccac catccggcca tgacgctgcc gtgcggcatg
     8101 atcgatgggc ttccggtggg cctgatgctg gtagggaaac actacgacga acccacaatt
     8161 taccgcgccg cccaagcgtt cgagcaaacg ggtgactgga aaaagatgta gaacgcgacg
     8221 cgttcccttt gcagggagaa tcgggatttc tatttgttgc ctttccgaag gaacggcagc
     8281 aaatccccga gcagttcgtc cggcgcttct tcgggaatga aatggccgca cggcaccggg
     8341 tggccgcgta cgttggcggc tttctccttc caggtgccga gcacgtcgaa cagggtgtgc
     8401 atcttgccgt actcgcccca gatcgccagc accgggcagg cgagcttttt ccccaggtcg
     8461 cgttcgtcgt cggcaatatc cacggtcgcc gccgcgcgat agtcctcgag acctgcgcgg
     8521 atcgtcccgg gcagcctgaa ggcgcgcagg tattcctgga acatcggctc ctcgatggag
     8581 tcctggcgaa agcacaacgc ccggaacata tgccgcagaa atggctcggc gctagcggca
     8641 agcatgatct cgggcagatc cggcacctgg aagaagaacc aatgccagta cgcggcggcg
     8701 agttccttgc cggtgtcccg gaaaacggta tgcgtgggca cgatgtcgag aacggcgagg
     8761 tttttcacct cgcccgggta atccatcgcc agccggtgcg ccacccgcgc gccgcggtcg
     8821 tgtccgacca gtccatagct ttcgaatccc agcgcggaca tcacttcgcg gatgtctttc
     8881 gccatgctgc gcttgtcgta accgtcgcgc ggcttgtcgg agtcgccgta cccgcgcaag
     8941 tcggggcaca ccaccgcgaa ctgatcgagc agggtgggca cgactttgcg ccacatctgt
     9001 ccggtctgcg gataaccgtg gagcagcacc accggatcgc ctttgccgcc cacccatgcg
     9061 ttgatgcgca cgccgttggc gcgaatggtc tgctgctgca ttcccggcat aaaggacata
     9121 ggtcagtgaa tgagtgaagg tgtgaaggtg tgaagggtga agggtgaagg gtaatagctc
     9181 tagaattttc tccgcttttc gccgaatcac gcaactgagc gatagggttt ctgtttttac
     9241 cccttcacac attcaccctt cacgccttca cttagaaccc agatatttgc gcaaccgctc
     9301 cacgccttcg gcaagtacag ggatcgactt cggatacgag aagcgcacgt gctgcttcgc
     9361 ccgatactcg ccgaagtcga ttcccggcgc gatcgccacg ccagctttct ccagcacgtc
     9421 ccggcagaac tgatagctgt cggacacgag gcgttcgcag ttcgcataga tatagaacgc
     9481 cccctccggc atcaccggga tgtcgaagcc caggctgcgc agttccggca ccaggaaatc
     9541 gcgctgcgcg cggaaagcat cgcggcgttg gtcgagtagt gcggtggtat cggggtggaa
     9601 cgcggcgagc gcggccttct gcgccacttc cgaggaagag atgaacaggt tctgcgccag
     9661 cttgtccacc gccggcatga ttgcctcagg caccaccatc caccccaggc gccagccggt
     9721 catgccgaaa tacttggaga agctgttgat gatgaagaca tcatcggcga actcgagcgc
     9781 actgcggtcg cgctgtccgt acgtaaggcc gtggtagatc tcgtccacga tcagcactcc
     9841 gccgcgcgcg cgcaccaccg cgtgaatgcg ctggagctca tcgtgcgcga tcagcgtgcc
     9901 ggtcggattc gagggcgaag cgatcagcac cgccgccgtt ttcggcgtcc aatgcttctc
     9961 caccaatttg gcggtgagct ggtaggcggt cgaagcatcg acagggacgc cgaccggatc
    10021 gccctccatg gtgcgcacga agtgccggtt gcacggatag ccgggatcgg tcatcagcac
    10081 cctctctccg ggcgaaagaa tcgcacccat cgtcagcagc agcgcgcccg aagagccggt
    10141 ggtcacggca atgcgcgaac gcggcacatc cacgccgtag cgcgtgcggt accactccga
    10201 gatcgcgtcc ctcaattcgg taatgcccag cgactgggta tagccgaccc ggccggcctg
    10261 gatcgcacgg acgccggcct ctgcaatcaa cggcggggtg ggtgcatcgg gctcgcccac
    10321 caccatgttc gagatgctcc gcccctgcgc ctccagctcc tttgcccgcg cgatgagtgc
    10381 catcacgtgg aacggctcga tgccgcccat gcgcgaagcg atgtgcgggc tggcattgcg
    10441 gggaagcttt tcggggcgat tcatccggtc ctcggattgt tgcgctgtcc gcgccatgct
    10501 cgattgccgg ttcggcggac tgaggctcga attttatgcc cccccaggcg tccgccgcaa
    10561 cgaagccggt tcaaaaaaca gcgccgagcg tctaccatgt cgatcctctc aacatttgat
    10621 ctgaacggga aaccggcaat gaagatcgcg atcatcggca gcggcggggt cggcggctat
    10681 ttcggagccc gcctggctgg gagcggacag gatgtcactt tcgttgcccg cggtgcgcac
    10741 ctcgaagccc tgcagaaaaa cggcctcaag gtgttgagcg cgctcggcga cctgcatcta
    10801 cccgaagtga aggccaccaa agatacgacg gtcatcggtc ccgttgacgt ggtcatgatc
    10861 gcggtaaagc tctggggcac cgaggaagcg gcgcagtccg ccaagccgct gatcggccct
    10921 aacaccgtcg cggtgtcttt ccagaacggc gtggtcgccg tggatacgct gctgccgatc
    10981 ctgggcaagg aacacgtcat gggtggggtg gcgaacatcg ccgcgctcat cgaagcgcct
    11041 ggcgtgatcc gccacaacgg caacatggcc aacctgttct tcggagaact cgacggcaaa
    11101 ccctccccac gatcgcaggc gcttctcgtt gcctgcaaaa tagccaacat ccaggccgag
    11161 ctggtcaccg atatcaacgg ggcgatctgg gaaaagttcg tgcgcctggt tacgctgtcg
    11221 gcactgactt cgctgacccg catgccggtc ggaccgatcc gctccgaccc cgatacccgc
    11281 gtactgatgc agcaggtaat ggaagaagtg gtggcgatcg gcaaagccag aggcgttaaa
    11341 ttcgataccg ggatcatcgc cgagcagatg gctcgggtcg atggctatcc ggctacgatg
    11401 gtcgcatcga tgtgcggcga tctgcgccgc ggcaaccggc tggaactgcc gtggctgtcc
    11461 ggcacagtgg ccaagctcgg cgaggaactc ggcatcccga caccggcgaa tcatttcgtg
    11521 tatgcggcgc tcaagcttta cgccaacggc cgccccgccg atgcgcaggt ctgaatctgt
    11581 taccacagag acacagaggc acagaggcac agaggcacag agaaaatctt gtatacgtgc
    11641 aaaaactgcg aatccatgtt agcgggagaa ctatcttggg cattcccaaa ctttttttcc
    11701 atcgcatggc ttggttccat gtttctctct gtgcctctgt gtctctgtgg tgaaagactt
    11761 ttgcggggag gttgaaatga aactgaaaat aaagaacatc cgtgccaggg cagtgatggt
    11821 gccgctgaag cgcccgccgg tatcggcgtc gggcgcgctg ccggaagcgg cgctggtgct
    11881 ggtggacgtt gaaaccggcg aaggcatcac cgggcattcg tatctggcgg cgtacggcaa
    11941 gccgatgctg aagcccctgg tcagcaccac cgaagccgtg gccgaaatga tcaagggcga
    12001 tgcgctggtg ccgtacgaga tcgacgccaa gcttcgcaag cgcttcacgt tgttcgacac
    12061 gcccgggctg ttgggccttg cgctcgcggg tatcgacatg gcggcatggg acgcctacac
    12121 caagggactc ggcgtgccgc tggtctgcgc gctgggcgga cagatcaagc cggtgcgcgc
    12181 ctacaacagt tgcggtttgt ggatcaagga tccggcgttg ctcgcggacg aagcggcgga
    12241 actgcttgcg gagggcggct acagcgcggt caagctgcgc atcggccggc cgaatttcaa
    12301 ggatgacctg accgccgtgc gcaatgtgaa gaagcgcatc ggcggcgaca tttctctgat
    12361 gaccgacttc aaccagagcc agacggtaac cgaggccatc ctgcgcggtc gcgcgctcga
    12421 cgatgaaggc ctgtactgga ttgaggaacc ggtccgccac gacaactacg ccggttgcgc
    12481 gaagattgcg ctggaggtga acacgcctat ccagattggc gagaacctgc tcaacacctt
    12541 cgagatgcag aaggcgatcg aagcaaaagc tgccgagtac tacatgcccg acgttcagcg
    12601 catcggcggc gtcaccggct ggatgcgtgc cgccgcgctt gcgcacgcgc accacctcga
    12661 gatgtccagc cacctgtttc cggaaatcag cgcccatctg ctggcggtga cgccgacctg
    12721 ccactggctg gagtacatgg actgggccaa tccgatcctg cgggagccgt tcgaggtgaa
    12781 aaacggcaac gtgatcatcc ccgaccgtcc gggcgccggc atagcctgga atgaggacgc
    12841 tgtccaacgg tacgctcctg cgtaaaaccg gctgacagtt atcggatagg gcgcgggatc
    12901 ggaggcaggc acgaatccaa atccttctcg cggtagaccg ctacgtcttc tcgcgtaggc
    12961 ccctgacgta tcccctcctt cgtcctacac ggtttcgaag gagggaacgc gtggccgggc
    13021 tggcggtatc tcggtccgcc tgcctgaccc gtaggcactg ttcgcagcag tcgaaaaccg
    13081 ctgaattccc cgtccgcgcg ctcccggcaa tgaacttacg ccgtcagggt gaaatttcgt
    13141 tgacggtgca tttttggcaa gcgtagagtt caccacacgg gtagcctgat cgccggggtc
    13201 tcgttgcctg attcagacga gcgttgacgc gccgcagcag accgcgacta cggacgcgaa
    13261 cttgagtgaa cgcgcttcgg cattgaacgc ggcagaccgt ttacgcaagc ctctgctgaa
    13321 ttcccgacgg atcggaccca acttttcgcg ggctgcgtat ttttcaactc gggaggagaa
    13381 aaaaatatga tgcgactcgg ctttttactt gccctgcctc tggtgctcgc ctcactgtct
    13441 tttgccgcgg acgattcgaa gaaaacgcga gtcggcacat gcacggatgc caagagccag
    13501 atggaatact tctgcaatcc cgccaatgcg gcgagcgaca gcatggtagc tatcggcacc
    13561 gcctgcacga atgccaggaa caacgtcaaa gaagcctgtg aagggattgt cgagccggac
    13621 aagaagtatg agttcgatga caagaagaag gagtaattac cccggcgccg caacttgaat
    13681 tgcactgccg aaggttctgt catgaaaagg aggtaccttt gaaagcacat tccttgatca
    13741 ccgcattggt cctcggactt gccgcgaccg tcgcatttgc ggcggaaaaa gacacgcgag
    13801 gcggcacgtg cgatgacgcg aagaaacagc acgagtacta ctgtgaaggc aagaatgatc
    13861 ccaacgatat tttcacccag gcgaatatcg cttgtaacaa cgccaagagg aatatggccg
    13921 cagcctgcga aggcaaggtc gcgccggatg ccgagtacaa gttcgag
//
LOCUS       DASQOP010000159         3205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1872885, whole genome shotgun sequence.
ACCESSION   DASQOP010000159 DASQOP010000000
VERSION     DASQOP010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1872885"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..1573)
                     /locus_tag="VE030_06505"
     CDS             complement(44..1573)
                     /locus_tag="VE030_06505"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003261917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HZF23103.1"
                     /translation="MKEQLIIFDTTMRDGEQSPGASMTREEKLRIGRQLERMRVNVIE
                     AGFPAASNGDFESVKAVAQAVKDSIVCGLARATENDIRRAGEAVKPANASRIHTFIAT
                     SPIHMQKKLRMQPDQVIEQAVKSVKLARSLCEDVEFSPEDAGRSEIDFLCRILEKVIA
                     AGARTINIPDTVGYTMPEQFSAMIRQLRERIPNADKVVWSVHCHNDLGLAVANSLSAV
                     MAGARQVECTINGLGERAGNASLEEVVMAVRTRQDFFNCDTRIDATQIVPASKMVAAV
                     TGFAVQPNKAIVGANAFAHESGIHQDGVLKSRETYEIMRAEDVGWSTNKLVLGKHSGR
                     NAFKSRLQQLGIELESEEALNAAFMRFKELADKKHEIFDDDLLMLASDDAVTQEHFKL
                     VSLLAHSETGEKPVARIVVSEDGREKQAESRGSGPVDATFKAIETIVASGADLQLFSV
                     NNITSGTDSQGEVSVRLSKSGRIVNGQGADTDIVVASAKAYLNALNKLHSKLERLNPQ
                     V"
     gene            complement(1832..2629)
                     /gene="pssA"
                     /locus_tag="VE030_06510"
     CDS             complement(1832..2629)
                     /gene="pssA"
                     /locus_tag="VE030_06510"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HZF23104.1"
                     /translation="MNHPAPKPQWLTRTGIRHRGIYWLPNAITTCALFAGFYAIVQAM
                     NLRFELAAAGVFAAMLFDALDGRVARLTRTQSEFGAEYDSLSDMVSFGAAPALVIYEW
                     SLKGMGRLGWVAAFIYCACAALRLARFNTNIDVVDKRYFQGLPSPAAAGLLAGLVWAM
                     EAYDVARADVPWLAWVLTLFAGFTMVSNIRFYSGKDINMRKSVSFGVVVMIALAVVAL
                     MQVANNLPELLFSLFAIYAASGYAMWAWEHLRKKPTAPPPASSGSDL"
     gene            complement(2636..>3205)
                     /locus_tag="VE030_06515"
     CDS             complement(2636..>3205)
                     /locus_tag="VE030_06515"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HZF23105.1"
                     /translation="VGYFWGWWSVPFWLAALFILQFFRDPPRNVPNDPDAVVSPADGR
                     IVAVEKSRDPYLDRDSLKISVFMNVFDVHSNRSPVDGEVKKVWYYGGTFLNAALDKAS
                     LENERNALWIRADRGVDITCVQVAGLIARRILCYVKPHEKLTRGQRFGFIRFGSRVDV
                     YLPPGATPRAAVGDKVYAGETILAWFPAN"
BASE COUNT          576 a         1008 c         1014 g          607 t
ORIGIN      
        1 aacaagcccg ctcatcgagc gggcttgttc gcagcggcga acgtcacacc tgcgggttca
       61 gacgttccag cttggaatgc agcttgttga gcgcattcag ataggccttc gccgaagcca
      121 cgacgatgtc tgtgtccgcg ccctgcccgt tgacgatgcg gcccgacttg gacagccgca
      181 cgctgacctc gccctgcgaa tcggtgccgc tggtgatgtt gttcaccgag aacagttgca
      241 gatccgcgcc gctggcgacg atggtttcga tcgccttgaa ggtcgcatcc accggaccgc
      301 tgccgcgcga ttcggcctgc ttttccctgc cgtcctcgga aaccacgatg cgggcgaccg
      361 gtttttcgcc ggtctcggaa tgcgccagca gcgacaccag cttgaaatgc tcctgggtca
      421 cggcatcgtc ggaagcgagc atcagcaggt cgtcatcgaa gatctcgtgc ttcttgtcgg
      481 cgagttcctt gaagcgcatg aacgcggcat tgagagcctc ttcggactcc agttcgattc
      541 ccagttgctg caggcggctc ttgaaggcgt tgcgcccgga gtgcttgccc agcaccagct
      601 tgttggtgct ccagcccaca tcctccgcgc gcatgatttc gtaagtctcg cggctcttga
      661 ggacgccgtc ctggtgaatt ccggattcgt gcgcaaaagc gttcgcgccg acgatggcct
      721 tgttcggctg caccgcgaag ccggtgaccg ccgcgaccat cttgctggcc ggtacgatct
      781 gcgtggcatc gatgcgggtg tcgcagttga agaaatcctg gcgggtgcgc accgccatca
      841 ccacttcttc cagcgaggcg ttgccggcac gctcgcccag gccgttgatg gtgcactcga
      901 cctggcgcgc gcccgccatc accgccgaca gcgagttcgc caccgccaga ccgaggtcgt
      961 tgtggcagtg caccgaccac accactttat cggcattggg aatgcgttcg cgcagttgcc
     1021 gaatcatggc tgaaaactgc tccggcatgg tgtagcccac tgtgtccgga atgttgatgg
     1081 tccgggcacc ggcggcaatc accttctcca ggatgcggca caggaaatcg atctcggaac
     1141 gcccggcgtc ctcgggcgag aactcgacgt cctcgcacaa cgatcgcgcc agcttgaccg
     1201 acttcaccgc ctgctcgatc acctgatcgg gctgcatgcg cagcttcttt tgcatgtgaa
     1261 tcggcgaagt ggcgatgaaa gtgtggatgc gcgaggcgtt ggcgggcttt accgcctccc
     1321 cggcgcggcg gatgtcgttt tcggtggcgc gcgcgaggcc gcacacgatg ctgtccttca
     1381 cggcctgcgc aaccgctttc accgactcga agtcaccgtt gctcgcggcg gggaagccgg
     1441 cctcgatgac gtttacgcgc attcgctcca gttgccggcc gatgcgcagc ttttcctcgc
     1501 gggtcatcga agcgcccgga ctctgctcgc cgtcgcgcat ggtggtatca aaaatgatca
     1561 attgttcttt catggtcgtt ctccgctgcg aaatcgtttc ggactggcgc ctcgctgtcg
     1621 gattgccgga cgcgggcgta ttggtgcctg tttttcctgc ttcgggcttt ttgcagaaac
     1681 ccggcaaact gttggggaag taggttttta gcgcccgggg cgcagcagca ggccgcccag
     1741 cagaatgccg ggaagaaggg aagcgagttg gccaagctgt tggggaagca tgtggcggac
     1801 tataaggagt tttccccggc gctgcaagct tttacagatc cgaaccggac gacgcaggcg
     1861 gtggggcggt cggctttttg cgcaggtgtt cccacgccca catggcatag ccggatgcgg
     1921 cgtagatcgc gaacaggctg aacagcagct ccggcaggtt gttggcgacc tgcatcagcg
     1981 ccaccaccgc aagcgcgatc atcaccacca ccccgaagga cacgctcttg cgcatgttga
     2041 tgtccttgcc gctgtagaag cggatgttgc tcaccatggt gaagcccgcg aacagcgtga
     2101 gcacccacgc cagccagggc acgtcggcgc gcgccacgtc gtaggcctcc atcgcccaca
     2161 ccagtcccgc caacaatccc gcggcagccg ggctcggcag cccctggaag tagcgcttgt
     2221 ccaccacgtc gatgttggtg ttgaagcgcg cgagccgcaa cgctgcgcag gcgcaataga
     2281 tgaaggcggc gacccagccg agccttccca tgcccttgag cgaccattcg tagatcacca
     2341 gggcgggcgc cgcaccgaag gacaccatgt cggaaagact gtcgtactcg gcgccgaatt
     2401 cgctttgcgt gcgggtgagc cgcgcgacgc ggccatcgag cgcatcgaac agcatggcgg
     2461 cgaacacccc tgccgcggcc agttcgaagc gcaggttcat cgcctggaca atggcataaa
     2521 agccggcgaa caacgcgcag gtggtaatgg cgttgggaag ccagtaaatg ccgcggtggc
     2581 gaatgccggt gcgggtgagc cactgcggtt tgggtgccgg gtgattcatg tccgattagt
     2641 tggccggaaa ccacgccaga atggtttccc cagcatacac cttgtcgccg accgcggcac
     2701 gcggggtcgc gccgggcggc aaatagacgt ccacccgcga accgaagcgg atgaagccga
     2761 agcgctgccc gcgtgtgagt ttttcatgcg gcttgacgta gcacaggatg cggcgggcga
     2821 tcagcccggc cacctgcacg caggtgatgt ccacgccgcg gtcggcacgg atccacagcg
     2881 cattgcgttc gttttcgagc gaagccttgt cgagcgcggc attgaggaac gtgccgccgt
     2941 agtaccagac cttcttcacc tcgccatcca ccgggctgcg gttggagtgc acgtcgaaca
     3001 cgttcatgaa cacgctgatt ttcagtgaat cgcggtcgag ataggggtcg cgggattttt
     3061 ccaccgcgac gatgcgcccg tcggcgggcg agaccacggc gtcgggatcg ttcggaacat
     3121 tgcgcggcgg gtcgcggaag aactgcagga tgaaaagcgc cgccagccag aacggaaccg
     3181 accaccatcc ccagaagtac ccgac
//
LOCUS       DASQOP010000160         2717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1676366, whole genome shotgun sequence.
ACCESSION   DASQOP010000160 DASQOP010000000
VERSION     DASQOP010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1676366"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VE030_06520"
     CDS             complement(<1..597)
                     /locus_tag="VE030_06520"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZF23106.1"
                     /translation="MQLLFLGSGSAHTVGADNFQSNMMLIADSGRRLLIDCGSDVRWS
                     LARQGLSHRDVTDIYISHLHADHIGGLEYVGFQTKFDPRCERPRLYIEASLAAPLWNC
                     SLRGGMGVISGTETELDTFFDVRIVTANQPFEWEGARLEPVPAVHVSSPRATVHSYGL
                     LIEHDGHRTFLTTDTQFTPGHLAACYASADLIFHDCETG"
     gene            666..1358
                     /locus_tag="VE030_06525"
     CDS             666..1358
                     /locus_tag="VE030_06525"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZF23107.1"
                     /translation="MADTLIDSLARVWLFNALANDDLVRLSQLAKTRVYKPRETIVAK
                     GDPATEFFVLLRGRAKVTAQGADGADTAINVMGPGEVFGEIGILDGQPRSASVTTLEE
                     CEMAVVDKRAFDGLLAASPPVAIKLLAVLAGRIRELTIRIEDRAFLDVPARLAKQLLW
                     LASNHGTESGGRVRIDLKLSQQELGDFIGATRESVNKNLRDWSRSGFIKHERDYLEIL
                     DLDALRAIAERR"
     gene            complement(1367..>2717)
                     /locus_tag="VE030_06530"
     CDS             complement(1367..>2717)
                     /locus_tag="VE030_06530"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZF23108.1"
                     /translation="SADFTERLVKALERIGPTLHLSAQRLDALLNRPGIAAAEEEDAA
                     GIRLTAWLDEQESHHQFLIYETDGTPSLWTRRCLRQADEILLVAHAGSDPAPGTVEKT
                     LLDTKDGISKARQNLVLLHPDGSRLPSGTSRWFANRNVQRNFHVRLDAENDFGRVARC
                     LGDAAIALVLGGGGARGLAHIGVVRALREAGVPIDMIGGTSMGAVMASLVAMDQDWDQ
                     MLETNREAWLRRKPHKEYSLPFISLIRSRRLDSMAKKIWGEIEIEDLWISFFCVSCNL
                     STSSTMIHERGLLWKAIRASASLPGVFVPVLSNGNILVDGGLVNNLPGDVMRERACRT
                     LIVVDVGSEHEFTFNLPEFPSPWQFLRSRILPFAARIEVPHIVDVLIRTTDVSSSQKT
                     RDVKRNADVCLRPPIDAYGVLQFESLDEIAEVGYRYTKSKMEDLRGKKSLAEMFEAM"
BASE COUNT          521 a          841 c          860 g          495 t
ORIGIN      
        1 gccggtctcg cagtcgtgaa aaatgaggtc ggcgctcgcg tagcaggcgg ccaagtgccc
       61 cggagtaaac tgggtatccg ttgtgaggaa cgttcgatgg ccgtcgtgct cgatcaacag
      121 gccgtagctg tggacagtcg cgcgcgggct ggaaacatgg actgcgggga cgggttcaag
      181 gcgggcgcct tcccattcga acggctgatt cgcggtgacg atgcgcacgt cgaagaaggt
      241 atcgagttca gtctcggtgc cggaaatgac gcccatgcct ccgcgcagcg agcagttcca
      301 gagcggagcg gccagagacg cttcgatgta gagacgggga cgctcgcaac gcgggtcgaa
      361 tttcgtctgg aatccaacgt attcgagccc gccgatgtga tccgcatgca gatggctgat
      421 gtagatgtcg gtaacatccc ggtgggaaag accttgtctc gccagcgacc agcgaacatc
      481 cgaaccgcag tcgatcagca ggcgccggcc gctgtcggcg atcagcatca tgttcgattg
      541 gaagttatcg gcgcccacgg tatgcgcgga gcctgaaccg aggaagagga gttgcatgtg
      601 ccgaagtcta acaagccgta tgcaggcttt cctatgaact gcttcacaga cgcaggccaa
      661 gaaccgtggc ggatacgctg atcgacagcc tggcgcgcgt ctggctcttc aacgcgctcg
      721 caaacgacga tctcgtccgc ctgtcgcaac tggcaaaaac cagggtgtac aagccgaggg
      781 agacgatcgt cgccaagggc gacccggcca ccgagttctt cgtgctgctg cgcggccgcg
      841 cgaaagtcac tgcgcagggc gccgacgggg ccgacaccgc aattaacgtc atgggtcccg
      901 gagaagtctt cggcgaaatc ggcattctcg acggccagcc ccgttccgcc agtgtaacca
      961 cgctggagga gtgcgagatg gcggtggtgg acaaacgcgc attcgacggt ctgctcgcag
     1021 cctcgccgcc agtcgcgatc aagctcctag ccgtgctcgc aggccgcatc agggaactca
     1081 ccatccgcat cgaggatcgc gccttcctgg acgttcccgc caggctggcg aagcaactgc
     1141 tatggctggc gagcaatcac gggaccgaga gcggcgggag agtgcggatc gatctcaagc
     1201 tgtcgcagca ggaactgggc gacttcatcg gcgcgacgcg ggaaagcgtg aacaaaaacc
     1261 tgcgcgactg gtctcgaagc gggttcatca agcacgagcg cgattacctc gaaattctgg
     1321 accttgacgc gctgcgcgcg attgccgaac gccggtaaac cgggccttac atcgcctcga
     1381 acatctctgc cagcgatttt ttcccccgca aatcttccat cttcgatttg gtgtagcgat
     1441 agccgacttc ggcgatctcg tcgagcgact cgaactgcag gacgccgtac gcgtcgatcg
     1501 gcggtctgag gcaaacgtcg gcgtttctct tgacgtcgcg cgtcttctgg ctgctgctga
     1561 catcggtcgt gcggatgagc acgtcgacga tgtgcgggac ttcgattcgc gctgcgaatg
     1621 ggaggatgcg gctgcgaagg aactgccagg gcgaaggaaa ctcaggcaga ttgaaggtga
     1681 attcgtgctc cgatccgacg tcgaccacga tcagcgtccg gcaggcccgc tcgcgcatga
     1741 cgtccccggg caggttgttg accagccctc catccaccag gatgttgccg ttgctcagga
     1801 ccggcacgaa cactccgggc agcgaggcgc tggcgcgaat cgctttccac agcaatccgc
     1861 gttcgtggat cattgtggag ctggtggaga ggttgcagga cacgcagaaa aaactgatcc
     1921 acaggtcttc gatctcgatc tcgccccaga ttttttttgc catgctgtcc agccgccggc
     1981 tgcggatcag cgagatgaac ggcagcgaat attccttgtg cggtttcctg cgcagccacg
     2041 cctcccggtt ggtctccagc atctgatccc agtcctggtc catcgccact agcgaagcca
     2101 tcaccgcccc catgctggtg ccgccgatca tgtcgatcgg cactcccgct tcgcgcagcg
     2161 cgcgaaccac gccgatatgc gccagtccgc gcgcaccgcc gccgccgaga accagtgcga
     2221 ttgcggcatc tcccaggcag cgcgccacgc gcccgaaatc gttctccgcg tcgaggcgga
     2281 cgtggaaatt gcgctggaca ttgcggttcg cgaaccaacg cgaggtccct gacggcaaac
     2341 ggctgccgtc ggggtgcaac agaaccaggt tctgccgggc ctttgagatg ccgtccttcg
     2401 tatcaagaag ggtcttttcc actgtgccgg gcgccgggtc cgaaccggcg tgcgctacca
     2461 gcagaatctc gtccgcctgg cggaggcaac gccgcgtcca gagggagggc gtgccatcgg
     2521 tttcgtagat gaggaactgg tgatgcgatt cctgttcgtc caaccatgcc gtcagccgga
     2581 tgccggccgc atcctcctct tcggctgcgg cgattccggg ccggttcagc agcgcgtcca
     2641 gccgctgcgc cgacaaatgc agcgtggggc cgatacgttc gagcgctttc accagccgct
     2701 cggtaaagtc cgcgctc
//
LOCUS       DASQOP010000161         5679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2680987, whole genome shotgun sequence.
ACCESSION   DASQOP010000161 DASQOP010000000
VERSION     DASQOP010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5679
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2680987"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VE030_06535"
     CDS             <1..479
                     /locus_tag="VE030_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HZF23109.1"
                     /translation="LVVVGGWYVSGHIGHLAEDPNTLQEAFVATNSGRMESFSFVSPM
                     AYTLDYLMLWTDKSKIITYGIASAAGVIAGSAAYALATRTFRWEGFRDAEDTASHIIG
                     GMLMGFGGICALGCTIGQAISGVSTLALGSIITFVFIVLGSAATMKLQYWRMARED"
     gene            501..576
                     /locus_tag="VE030_06540"
     tRNA            501..576
                     /locus_tag="VE030_06540"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:534..536,aa:Lys,seq:ttt)
     gene            607..1605
                     /locus_tag="VE030_06545"
     CDS             607..1605
                     /locus_tag="VE030_06545"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HZF23110.1"
                     /translation="MNAAWYEKQGPARHVLVVGTLPDPEPEPGEVRIRVAASGINPGD
                     VKKRQNAFGYGMPFPRVIPHSDGAGTIDRIGQGVARPRIGERVWCYGAQSYRAFGTAA
                     EYVVVPSSQAVPLPDGVAFEQGACLGIPGITAHRAVHAAGPVAGRTLLVQGGAGAVGV
                     CAVGLARLAGARVLATVRSDADAAVARAAGAHEVVQFGGCSRDEFIRDLRALAPGGMD
                     HVVEVAFGANIEADCEVLANDGSIAAYATETATPAIPFWPLVFKNVRIFFLGSDDFPQ
                     AAKAAAAESINAMLAGGWPGFRIDKRVSLANIAEAHEYLEQRHSNGRAVVITASSE"
     gene            complement(1815..4001)
                     /locus_tag="VE030_06550"
     CDS             complement(1815..4001)
                     /locus_tag="VE030_06550"
                     /inference="COORDINATES: protein motif:HMM:NF023683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23111.1"
                     /translation="MKRALLSALMIFTLTSSAWAQHSPAVHARFDLASVSGAPFPSDL
                     YTIADPSNRTGRRVNLPLPGCSARPSDCNDLAVVNTLDGFNQMPRISVPFDGDIDAES
                     VSSSSMFIAELAQEGEEAIVVKTVGINQVVWNPATLTLHVESDEVLRQTARYAVIVTN
                     GIHDASGRPVQASDAFDDFRHDLNYGQTRSEELKEYRKALITTLAAVERLGVSKRDVV
                     ALSVFTTQTATATLENVRDYLKGSPPPPPAKFDIGPLGSRAVFARSSLQTITFRQQMG
                     GTIPFVNATVPLPALNAYTTGAVSTVAWGTFVSPQFIDSNAVMTAHGTLEVPTQLGEA
                     TLEFVLFLPSGTRPASGWPVAFLGHGGTTNIHTAAVWNFASSLAAHGFASIAINAIGR
                     GFGPLGTLIIKKTDGTTVEINAGGRTIDQDGNGVYVNQEGAAAQAPYLIVGQRDSVRQ
                     VAIDYMQLVRVIQVGIDVDNDGSPDLDASNMVFLGNSFAVGYEMCFLAVEPDVGIAAV
                     GSPGGLPGRFDLLSMRPSGRTMVGAGLAARTPSLLNAAYGLTSLGGIPATAPFYNENI
                     PLRDLAPVTNTIPGAMAIQEYFDRIEWVQASSDAATQAPHLEFEPLTGVPAKSILVLY
                     AKGDMTAPNPRTTALVRAGVLREKTIFFRNDIAFAEDPTTVPKDPHTFLQPLTTAGIT
                     GQIARGGQATIAQFFVSGGTTINHPEPARFFEFPIVIMPEDYSYIP"
     gene            4108..5046
                     /locus_tag="VE030_06555"
     CDS             4108..5046
                     /locus_tag="VE030_06555"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZF23112.1"
                     /translation="MSLNPKALVALGAVITEGTVTAAAQRMHRTQPVVSRLIAQLESA
                     VGFALFRREGGRLLPTAEGLSFYRESERAFAVLSEIESAARNIRERRDVPLRILAQAH
                     VAHGLLQLALGPFCARNPGFRFSIEIRLREYINHWIANRQFDVGFAPQPLNDPRVDAE
                     PLVKAPVCVVLPGRHPLARKQRIGIADIGKEPIIATRPGSPLRTRLDALFAAAGMRPV
                     VRGETATSLSACQLAGQGAGATIAEPFICNLFIEDPTICIRLLHPRMDIEYSVLRPSG
                     QVPSPIVQQFIESVRQTAREVVEAVVRHAAGRRRSR"
     gene            complement(5073..>5679)
                     /locus_tag="VE030_06560"
     CDS             complement(5073..>5679)
                     /locus_tag="VE030_06560"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HZF23113.1"
                     /translation="SIPYVLVVHPSVPARRMSDLIELARARPGALNFGSAGPGSPLHL
                     AMELLKLQAQIDLHHVPYRGAAPAQTALLGGEVDTAFLVPPLVQGHVKTGSLRALAVS
                     STARSPMFSEVPTLQEAGITGYEALQWHGFFVPAKTPAPVVDRLYREVATALAAPDMA
                     KRLAAEGASVVGSTPAQFAAFFRAEVGKWSEVVRVSGVKFD"
BASE COUNT          993 a         1782 c         1879 g         1025 t
ORIGIN      
        1 ggctggtggt ggtcggcggc tggtatgtca gcgggcacat cggccacctg gctgaagacc
       61 cgaacacgct gcaggaggct ttcgtcgcca ccaattccgg gcgcatggaa tcgttctcgt
      121 tcgtctcgcc gatggcgtac acgctcgact acctgatgct gtggaccgac aagtcgaaga
      181 tcatcaccta cggcatcgcc tccgcggccg gcgtgatcgc cggctccgcc gcgtatgcgc
      241 tcgccacacg gaccttccgc tgggaaggtt tccgcgatgc cgaggacacc gcgagccaca
      301 tcatcggcgg aatgctgatg ggcttcggcg gcatctgcgc gctcggctgc accatcggcc
      361 aggcgatctc cggcgtctcg accctcgcgc tgggctcgat catcactttc gtgttcatcg
      421 tgctcggctc cgccgcgacg atgaagctcc agtactggcg aatggcccgc gaggactgat
      481 aaaatttcgc cctttcccgc gggtcgttag ctcagctggt agagcagcgg acttttaatc
      541 cgttggtcgg cggttcgaat ccgccacgac ctaccaatct ctttcaagcg gttgccgcag
      601 agctggatga acgccgcatg gtacgaaaag cagggtccag cccgacacgt tctcgtcgtc
      661 ggcacgctgc cggaccctga gccggagcca ggcgaggtgc gtatccgggt cgctgcatcc
      721 ggaatcaatc cgggtgacgt taagaagcga cagaacgctt tcggctacgg catgccgttc
      781 ccgcgcgtga ttccccacag cgatggagcg gggaccatcg accgcattgg gcaaggcgtc
      841 gcgcgcccgc gaatcggcga gcgcgtgtgg tgctacgggg cgcagtccta tcgcgcgttc
      901 ggcaccgcgg ccgagtatgt cgtcgtgccc tcctcgcagg ccgtaccttt gccggacggc
      961 gttgcgttcg agcaaggcgc ttgcctcggc ataccgggca tcaccgcgca tcgagcggtt
     1021 catgcggcgg ggccagttgc tgggcgtacg ctcctggtcc agggtggcgc cggtgcagtc
     1081 ggcgtctgcg cggtcggtct ggcgcggctc gcaggcgctc gggtactcgc caccgtgcgt
     1141 tccgatgccg acgccgcggt agctcgtgcg gccggcgcgc acgaggttgt ccagttcggc
     1201 gggtgttccc gcgatgagtt cattcgagat ctgcgcgctc ttgcgccggg cgggatggat
     1261 cacgtagtgg aagtggcctt cggcgcaaac atcgaggcgg actgcgaagt tctggcgaat
     1321 gacggttcga ttgctgccta cgcgacggag acggccacgc cggcaatccc attctggccg
     1381 ctggtcttca agaatgtccg gatcttcttt ctcggcagcg acgactttcc ccaggccgcg
     1441 aaagccgctg ccgctgaaag catcaacgcc atgctggctg gcggatggcc gggattcagg
     1501 atcgacaagc gggtttcgct cgccaacatc gcggaggcgc atgagtatct cgagcagcgc
     1561 cacagcaacg gacgagcagt cgtaattacc gccagcagcg aataagacgt ggggaaggcc
     1621 gccagctttt aatccgttgg tcggcggttc gaatccgcca cgacctacca atcctcttct
     1681 caagccgctt ccggcaccac tgcgaaggcg ctgccgaagg cggaactcat cgccgttgga
     1741 aatatcgccg tgcgcgggct cgccgaacca gtcagcgtct tttcgatatt gccaaagccg
     1801 cgcgccctgt gcgactacgg gatgtaggag tagtcctccg gcatgatcac gatcggaaat
     1861 tcaaagaagc gggccggttc ggggtggttg atggtggtcc cgcccgagac aaagaactgg
     1921 gcgatcgtcg cctgaccgcc gcgggcgatc tgaccggtga tgccggcggt cgtgagcggc
     1981 tgcaggaagg tatggggatc cttgggtacc gttgttggat cctcggcaaa ggcgatgtcg
     2041 ttgcggaaga agatcgtctt ttcgcgcagc acgccagcgc gtaccaaggc cgtggtcctg
     2101 ggattgggag ccgtcatgtc gcccttggcg tacaggacga ggatggattt cgcgggaaca
     2161 ccggtcagag gctcgaattc gaggtgggga gcctgcgtgg ccgcgtcact gctcgcctgc
     2221 acccactcga tgcggtcgaa gtactcctgg attgccattg caccggggat cgtgttggtc
     2281 acgggcgcaa gatcgcgcag cgggatgttc tcattgtaga acggcgcagt ggcagggatg
     2341 ccgccgagag atgtcagacc ataggccgcg ttcagaagcg acggagtgcg cgcggcgagc
     2401 ccagcgccga ccatcgtgcg tcccgagggg cgcatgctga gcaggtcgaa gcggccggga
     2461 agtccgccgg gcgagccaac cgcggcaatg ccgacgtcgg gctcgaccgc gaggaagcac
     2521 atctcatagc cgaccgcgaa cgagttgccg agaaacacca tgttcgaggc gtcgaggtcg
     2581 ggtgagccgt cattgtcgac gtcaattcca acctggatga cacggacgag ttgcatgtag
     2641 tcgatagcga cctgtcgcac agagtcccgc tggccgacga tcaggtaggg cgcctgcgca
     2701 gccgcgcctt cctggttcac atagacgccg tttccgtcct gatcgatcgt tcgtccgcca
     2761 gcgttgattt cgaccgtcgt cccgtcggtt ttcttgataa tcaacgtgcc cagcggacca
     2821 aagccacggc cgatggcatt gatggcgatc gaggcaaaac cgtgggccgc gagcgagctg
     2881 gcaaagttcc aaaccgcggc ggtgtggata ttggtggtgc caccgtgccc gaggaaggcg
     2941 accggccaac cagacgcggg gcgtgtgccg ctgggcagga atagcacgaa ctcgagggtt
     3001 gcctcaccga gctgcgtcgg cacctcaagc gtcccgtggg cggtcatcac cgcattgctg
     3061 tcgatgaact gcggagagac gaaggttccc cacgcgacgg tgctcacggc tccggtggtg
     3121 taggcgttca gagcggggag cggcactgtc gcgttcacga aagggatcgt cccccccatc
     3181 tgctgcctga aggtgatggt ttggagcgat gaccgggcga agaccgcgcg tgacccgagg
     3241 ggaccgatat caaatttggc gggaggtggg ggcggggagc ccttcaggta atcgcgaacg
     3301 ttctcgagcg ttgcggtggc ggtctgggta gtgaaaacgc taagcgccac cacgtctcgc
     3361 ttggagacgc ccaaccgttc gacggccgcc agagtcgtaa tcagcgcttt tcggtactcc
     3421 ttgagctcct cgctcctggt ctgtccataa ttgaggtcgt gcctgaaatc gtcgaaggcg
     3481 tccgatgctt gcaccggccg ccccgacgcg tcatggatcc cgttggtgac gatcactgcg
     3541 tagcgggcgg tttggcggag cacttcatcg gactcgacgt gcagggtgag tgtcgccgga
     3601 ttccagacaa cctggttgat tccaacagtc ttgacgacga ttgcctcctc tccttcctgc
     3661 gccaactcgg ctatgaacat ggagctggag gaaacgctct cggcatcgat atcgccgtca
     3721 aacgggacgg agatgcgcgg catctggttg aagccgtcca gcgtgttcac cacggcgaga
     3781 tcattgcaat ccgacggtcg tgccgagcaa cccggaagcg gcaggttcac ccttcgtccc
     3841 gttctattgc tcgggtccgc aatggtgtag agatcgctcg ggaaaggggc gccgctgacg
     3901 gaagccaagt caaaccgggc atggacagct gggctgtgct gtgcccacgc cgacgaagtc
     3961 agcgtgaaga tcatcagcgc cgaaaggagc gctcgtttca tggttcgcct cccccccggc
     4021 ggcgatcttg tccctttcac cttatgcagt ccaatgcaaa attaggcggg atcatgcaga
     4081 attcgcatgg ttggaaacgg agctcccgtg agcctcaatc cgaaagcgct cgttgccctg
     4141 ggggcggtga tcactgaagg cacggtcaca gcggcggcgc agcgcatgca ccgcacgcag
     4201 ccggtcgtga gccgcctgat cgcgcagctc gaatcggcgg tcgggtttgc gctcttccgg
     4261 cgcgagggcg ggcggctcct gcctacggcg gagggcctga gcttttaccg cgaaagcgag
     4321 cgcgcctttg ccgtcctctc cgaaatcgaa tcggcggcgc ggaacatccg cgagcggcgc
     4381 gatgtaccgc tgcgcatcct tgcgcaggcg catgtcgccc acggcttgct gcagctggcg
     4441 ctggggcctt tctgcgcccg caaccccggt tttcgcttct ccatcgagat ccgcctgcgc
     4501 gagtacatca accactggat cgcaaaccgg cagttcgacg tgggatttgc gccgcagccg
     4561 ctgaatgatc ccagggtcga cgcggagccg ctggtaaagg cgccggtatg cgtggtcctg
     4621 cccggccggc atccgctagc ccggaagcag cgcatcggca tcgccgacat cgggaaggag
     4681 ccgatcatcg cgacccgccc cgggtctccc ctacgcaccc gcctggatgc gctgtttgcc
     4741 gccgccggca tgaggccagt cgtgcgcggc gaaaccgcaa catcgctttc cgcgtgccag
     4801 ctcgccggtc agggtgcggg cgcgacgatt gccgaaccgt tcatctgcaa ccttttcatc
     4861 gaagacccga cgatctgcat ccgcctgttg catccccgca tggacatcga atattccgtg
     4921 cttcgccctt ccgggcaggt ccccagtccg atcgtccagc agttcataga aagcgtaaga
     4981 cagacggccc gggaggtggt cgaggccgtc gtgcgccacg cggctggacg gcgccggagc
     5041 cgataagccg ccgtggtcgt ccagctggat gttcagtcga acttcacccc ggaaaccctc
     5101 acgacttcgg accacttgcc gacctcggcg cggaaaaagg cggcgaactg cgccggcgtg
     5161 ctgccgacga cgctggcgcc ttcggccgcc aggcgctttg ccatatccgg cgcggcgagc
     5221 gccgtcgcga cctcgcggta caggcgatcg acgacgggcg ccggcgtttt cgccggaacg
     5281 aagaagccgt gccattgcag cgcctcgtag ccggtgattc cggcttcctg gagcgtcggc
     5341 acctcggaaa acatcggcga acgcgcggtg gaagacactg ccagggcgcg caaacttccc
     5401 gtcttcacgt ggccctggac cagcggcggc accagaaacg ccgtgtcgac ttctccgccg
     5461 agcagcgcgg tctgagccgg ggccgccccg cgatacggga cgtgatgcag gtcgatctgc
     5521 gcctgcagct tgagcaactc catcgccaga tgcagcggcg agccgggccc ggcggagccg
     5581 aaattgaggg ctcccggtct cgccctggcg agttcgatca ggtcgctcat ccgccgcgca
     5641 gggacggacg ggtggaccac cagcacatag ggaatcgaa
//
LOCUS       DASQOP010000162         4280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1677490, whole genome shotgun sequence.
ACCESSION   DASQOP010000162 DASQOP010000000
VERSION     DASQOP010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1677490"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2197)
                     /locus_tag="VE030_06565"
     CDS             complement(<1..2197)
                     /locus_tag="VE030_06565"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HZF23114.1"
                     /translation="MDLELESAAIEYHEQPTPGKISVVPTKGLTNQRDLALAYSPGVA
                     FACKAIEADPSRAAQLTSRANLVGVVTNGTAVLGLGNIGPLAGKPVMEGKACLFKKFA
                     GIDVFDIELAENDPDKLVDMIAALEPTLGGINLEDIKAPECFYIESKLRERMKIPVFH
                     DDQHGTAIIVGAAVTNGLKVVGKDIAKVKLVCSGAGASAIACLDLLVKLGLKRENIIV
                     CDSKGVIFEGRDKSMEPNKARYARKTDACRLAEVMPGADIFLGLSGPGAMKQEWIKTM
                     AAKPLVLALANPTPEILPEEVKAVRPDAVIATGRSDYPNQVNNVLCFPFIFRGALDVG
                     ATKITEEMKLACVKAIAELTQAEQSDVVAMAYEGEELSFGPEYIIPKPFDPRLITRIA
                     PAVARAAMDSGVATRPITDFDAYRQQLNNFVYHSGFVMKPVFAAAKAAPRRVIYAEGE
                     DERVLRAVQVVVDERLANPILIGRPEVVEMRLERFGLRIKPGKDFELVNPESDARYRE
                     VWTEYYKAMNRKGVSPDDARVHVRQSTTLIGAMLLRRGDGDAMLCGTFGRHKDHLRHI
                     ANVIGLKTGASIFAAMNVLLLAKRTLFISDTYVNEDPGVEQLAEIAIMAAEEVKRFGV
                     APRVALLSHSNFGSDDSPAALKMSRARDLIEKLAPDLEVEGEMHGDAALSESIRNHVH
                     PESRLKGEANLLIMPNIDAANISFNLLKMTGGEGVTVGPILLGAAKPVHI"
     gene            2401..4227
                     /locus_tag="VE030_06570"
     CDS             2401..4227
                     /locus_tag="VE030_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HZF23115.1"
                     /translation="MKLETPEQVTTAKPAALARLRALAVLWRFATPYRARMLVFALAL
                     VVAAACFLVIGQGLKQVIDRGFAGGDPQALNQALFFLLGVIVVMSVATWVRFYLISWL
                     GERVIADIRRAVFSRLLSLSPGWFEQARTGDVISRLTTDTALLEQVVGTSVSMALRNG
                     LLGLGALAMLMLTSWKLTALVLLMVPVVIGPIVVFGRQVRKLARASQDRVADLGSYVG
                     EALHEVRTVQAYGHEDEDRRLFGSRAEAAFDTARSRIRVRASLISAVIVLVFTGIGVI
                     LWIGGHDVLTGSITPGELSAFVFYAAMVASAAGALSEVIGDLQRGAGAAERLIEILDT
                     RPEITAPAQPLALPEPPIGSVQLQAVTFHYPSRPNQAALQDFSLDVRPGESLALVGPS
                     GAGKSTVFQLLLRFYDPERGTVRVDGVDVKRVDPQALRKRIALVAQEPAIFAASVAEN
                     VRYGRPDASDEEVRRACHAAFATEFVEMLPQGFDTWLGERGVRLSGGQRQRIAIARAI
                     LRSPALLLLDEATSALDAESEQVVQKALERLMEGRTTIVIAHRLATVQKCDRIVVMDH
                     GRIVAVGSHGELVRQGGLYARLAALQFDQPLELTPRSSADPV"
BASE COUNT          664 a         1346 c         1415 g          855 t
ORIGIN      
        1 ggatgtgcac cggcttggcg gcgcccagca gaatcggccc cacggtcacc ccttcgccgc
       61 cggtcatttt cagcagattg aaggaaatgt tcgcggcgtc gatattgggc atgatcagca
      121 ggttcgcttc gcccttcagc cgggattccg gatgcacgtg gttgcggatc gattccgaaa
      181 gcgcggcatc tccgtgcatt tcgccttcga cctcgagatc cggggcaagc ttctcgatca
      241 ggtcgcgggc ccggctcatt ttcagcgcgg cgggtgagtc atcgctgccg aagttcgaat
      301 gggacagcag ggcgaccctg ggcgcgaccc cgaagcgctt cacttcttcg gccgccatga
      361 ttgcgatctc ggccagttgt tcgacgcccg ggtcttcgtt gacataggtg tccgagatga
      421 acagcgtgcg ttttgccagc agcagcacgt tcatcgcggc gaatatgctc gcgccggttt
      481 tcaggccgat gacgttggcg atatggcgca ggtggtcctt gtggcggccg aacgtgccgc
      541 acagcatggc gtcgccgtcg cccctgcgca gcagcatggc gccgatcagc gtcgtggact
      601 ggcgtacgtg caccctggcg tcgtcggggg aaacgccttt gcggttcatc gctttgtagt
      661 attcggtcca tacttcccgg taacgcgcgt cgctctccgg gttgacaagc tcgaaatcct
      721 tccccggctt gatgcgcagc ccgaaacgtt cgagccgcat ttccaccact tccggccgcc
      781 cgatgaggat cggattggcg aggcgctcgt ccaccaccac ttgcacggcg cgcagcacgc
      841 gctcatcctc gccctccgcg taaatcaccc gccgcggtgc ggccttcgcc gcggcgaaca
      901 ctggcttcat cacgaacccg gagtggtaaa cgaagttgtt gagctgctgg cggtaagcgt
      961 cgaaatccgt gatcgggcgc gttgccacgc cgctgtccat tgccgctctc gccaccgccg
     1021 gtgcgatccg ggtgatgagc cgcggatcga aaggcttggg aatgatgtac tccggaccga
     1081 atgacaattc ctcgccttcg taggccatgg ccaccacgtc cgactgctcg gcctgggtga
     1141 gttcggcgat cgccttcacg caggccagtt tcatttcctc ggtgatcttg gttgcgccga
     1201 cgtcgagtgc gccgcggaag atgaacggga agcacagcac gttgttcacc tggttgggat
     1261 aatcggagcg gccggtggcg atcaccgcat cgggtcgcac cgctttcact tcctctggca
     1321 ggatttccgg cgtcgggttg gcgagcgcca gcaccagcgg cttcgccgcc atggtcttga
     1381 tccactcctg tttcattgcg cccggacccg agagtccgag gaagatgtcc gcgccgggca
     1441 tcacctcggc aagcctgcac gcgtcggtct tgcgcgcgta acgcgccttg ttcggctcca
     1501 tgctcttgtc gcggccttcg aatatgacgc ctttcgagtc gcagacgatg atgttctcgc
     1561 gtttcagccc gagtttcacc aacaggtcga ggcaggcgat cgccgaagcg ccggcgccgg
     1621 agcacaccag ttttaccttc gcaatgtcct tgcccaccac cttgagcccg ttggtcaccg
     1681 ccgcgccgac gatgatcgcg gtaccgtgct ggtcgtcgtg aaacaccgga atcttcatgc
     1741 gctcgcgcag tttgctttct atatagaagc actcgggcgc cttgatgtcc tcgagattga
     1801 tgccgcccaa agtgggttcc agcgcggcga tcatgtcgac cagcttgtcc gggtcgttct
     1861 cggccagctc gatgtcgaat acgtcgatgc cggcgaattt cttgaacagg cacgccttgc
     1921 cttccatcac cggcttgcct gccagcgggc cgatgttgcc caggccgagc accgcggtgc
     1981 cgttagtcac cacgccgacc agattggcgc gtgaggtcag ttgtgccgcg cgcgagggat
     2041 ccgcttcgat ggccttgcag gcgaacgcga cgcccggcga atacgcgagc gcgaggtcgc
     2101 gctgattggt gaggcccttg gtgggaacga cggagatttt ccccggggtc ggctgctcgt
     2161 ggtattcgat cgccgctgac tcaagttcca aatccattct tgtctcctct gggtatcgtt
     2221 gtaggttcgc tgcaccaacg gtcggcgaaa ccatcttttt cggctgtctg cagccgataa
     2281 gatactgcgg gttgcggtcc gatgggtggc ttcgttgtcc cggacggcga aatgcgcggg
     2341 atcgaggttt atgatgcgca gggtcagaat gcgcggacag gtgcccggat agccgagccg
     2401 atgaaacttg agaccccgga acaagtcacg acggcgaagc cggcggcgct cgcgcgcctg
     2461 cgcgcgctgg cggtgctgtg gcgcttcgct acgccatacc gcgcgcgcat gctggtgttc
     2521 gcgctggcgc tggtggtcgc ggcagcatgt ttcctggtga tcggccaggg actcaagcaa
     2581 gtcatcgacc ggggattcgc gggcggcgat ccgcaggcgc taaatcaggc gctgttcttc
     2641 ctgctgggcg tgatcgtggt gatgtcggtc gcaacctggg tgcgcttcta tctgatttca
     2701 tggctgggcg agcgggtgat cgccgacatc cgccgcgcgg tcttctcgcg cctgctgagc
     2761 ctgtcgccag gctggttcga gcaggcgcgc accggcgacg tgatttcgcg gctgaccacc
     2821 gataccgcgc tgctggaaca ggtggtcggc accagcgtgt cgatggcgct gcgcaacggg
     2881 ctgctggggc tgggcgcgct ggcgatgctg atgctgacca gctggaagct tacggctctg
     2941 gtgctgttga tggtgcccgt ggtgatcggg ccgattgtgg tcttcggccg acaagtgcgt
     3001 aaactcgccc gagccagcca ggatcgcgtg gcggatcttg gcagttatgt gggcgaggcc
     3061 ctgcatgaag tgcgcacggt gcaggcctac gggcacgaag acgaggaccg gcgcctgttc
     3121 gggagccgcg ccgaagcggc gttcgatacc gcgcgcagcc gcatccgggt gcgcgcttcg
     3181 ctgatctccg cggtgatcgt gctggtgttc acaggcatcg gagtcattct ctggatcggc
     3241 gggcatgacg tgctgacggg cagcattacg cccggcgaac tgtcggcctt cgtgttttac
     3301 gccgcgatgg tggcgagcgc tgccggcgcg ctgagcgagg tgatcggcga cttgcagcgc
     3361 ggcgccggtg cggccgagcg gctgatcgag atcctcgaca cgcgtccgga gatcaccgcg
     3421 ccggcccagc cgctcgcgct gcccgagccg ccgatcggat cggtacaact gcaggcggtg
     3481 actttccact acccgtcgcg tcccaaccag gcggcattgc aggatttttc gctcgatgtc
     3541 cggccgggag agagtctcgc gctggtcggc ccttcgggcg cgggcaagtc cacggtgttt
     3601 caactgctgc tgcgcttcta cgatcccgag cgcgggacgg tgcgagtcga cggcgtggac
     3661 gtaaagcgtg tcgatcccca ggcgttgcgc aagcgtatcg cgctggtggc gcaggagccc
     3721 gccattttcg cggccagcgt tgccgagaat gtacgctacg gccgcccgga cgcctccgac
     3781 gaagaagtcc ggcgtgcctg ccatgcggca ttcgcgacag agttcgtcga gatgctgcct
     3841 cagggattcg atacctggct gggcgaacgc ggcgtgcggc tttccggcgg ccagcgccag
     3901 cgcatcgcaa tcgcgcgcgc catcctgcgc agccccgccc tgctgctgct cgacgaggcc
     3961 acatccgccc tcgacgcgga aagcgaacag gtggtccaga aggcgctgga acgcctgatg
     4021 gaaggccgca ccaccatcgt catcgcccac cgcctggcaa cggtgcagaa atgcgatcgc
     4081 atcgtggtga tggatcatgg ccggatcgtc gccgtcggga gccatggcga gttggtgcgc
     4141 cagggcggac tctacgcccg gcttgccgcc ttgcagttcg accagcctct cgagttgacg
     4201 ccgcgctcta gcgctgatcc agtttgagtt cgatgcgccg attgcgacgc aagatatcat
     4261 cgctgtcgcc ttcggccagg
//
LOCUS       DASQOP010000163         3347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2280786, whole genome shotgun sequence.
ACCESSION   DASQOP010000163 DASQOP010000000
VERSION     DASQOP010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2280786"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VE030_06575"
     CDS             complement(<1..233)
                     /locus_tag="VE030_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23116.1"
                     /translation="MRRQFLATAASFSIIVPAYSQTHSDLLIDEVVVTATRFEDISSD
                     KPLNMTVITSEEIRHSPARTLPQLLSYQSGIGGR"
     regulatory      complement(349..671)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            846..1046
                     /locus_tag="VE030_06580"
     CDS             846..1046
                     /locus_tag="VE030_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23117.1"
                     /translation="MEAELNALDDKVTQLAQLCQKLRKDNNQLRQQLVTAQSESKRLA
                     EKVNTATTRLEGLLNQIPDGAE"
     gene            1043..1354
                     /locus_tag="VE030_06585"
     CDS             1043..1354
                     /locus_tag="VE030_06585"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HZF23118.1"
                     /translation="MSKEARGLDVNIMGREFRVSCTEEERRDLLKAVDYLNDKMREIR
                     DTGKVIGVERIAIMAALNISHEFLSTHMNGGFDVGEFKRRMLSMQAIIDQAMNGQDEL
                     F"
     gene            1374..1550
                     /gene="ssrS"
                     /locus_tag="VE030_06590"
     ncRNA           1374..1550
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VE030_06590"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            1677..2132
                     /locus_tag="VE030_06595"
     CDS             1677..2132
                     /locus_tag="VE030_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HZF23119.1"
                     /translation="MNYWLMKSEPDVFGIDHLAAAPEQTAAWWGVRNYQARNFMRDRM
                     KVGDRAFFYHSSCAEPGLAGIVRVSRPAHPDMTQFDRKSTYFDPKATRDSPRWFNVDV
                     TLVKKTRLVGLGELRSHPGLASMRVLQRGNRLSITPVEAREWDFIMGIL"
     gene            2149..2943
                     /locus_tag="VE030_06600"
     CDS             2149..2943
                     /locus_tag="VE030_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZF23120.1"
                     /translation="MEWWIVYLLMGLFVGFFAGMLGVGGGLILVTLMVFLFSLQGFPA
                     DRVVHIALGTSLTSIVFTSISSLRAHHRHGAVRWDIVKMATPGLVAGTLLGTFVADRM
                     ASKYLAIFFVAFVYYSAVQMFADMRPKPTRTLPGLAGMSVAAIITGIISSLVGAGGGL
                     LSIPLMTMCNVPMRNAVGTSAALGLPIAIAGAAGYIYNGLGQDHLPPLTLGYVYVPAL
                     VGIVIGTFVTVPAGAKAAHTMPVPRLKKIFSVILFLMATKMLWTLF"
     gene            complement(2947..>3347)
                     /locus_tag="VE030_06605"
     CDS             complement(2947..>3347)
                     /locus_tag="VE030_06605"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HZF23121.1"
                     /translation="DLADATAPQRRCLAHFETLRLALNRELQLGLLEFECHFSRYAQG
                     AFYRKHLDQFRGDRRRRLSCALYLNENWGREDGGELRLYLDAGEAGKFKDVLPVGGTL
                     VLFLSERFAHEVLPGKRERLSLAGWFKTRS"
BASE COUNT          659 a         1001 c         1028 g          659 t
ORIGIN      
        1 cgtcccccga tgccagactg atacgaaagc agttgcggca gcgtgcgggc tggagaatgt
       61 cggatttctt cggaagtgat gacggtcatg ttcagcggct tgtcgctcga gatatcctcg
      121 aatcgcgtag ccgtgacgac tacctcgtcg atgagcaggt cagaatgggt ttgcgaataa
      181 gccggaacga tgatgctgaa actggcagcg gttgccagaa attggcgtct cattgagatc
      241 tccctgattt gcatgcccgc cgcatgcggg ttgatgacag ggagttcccg ggggatgagg
      301 gacgagcggc aggtgcagct ccgctccgga tacccgcccc gcgcggaaac tccgttcgcg
      361 ttccagggcc ggtatccggg cttgcgaacc gggcgtgccg ccttcccatg ggatattccg
      421 gctttcagtc agccggcgtc gcccacagtg gcatcgtggc acgcccctac ggtcgcttac
      481 cgttgcgggg gcagcacagg aataaggtga ccactcggtt ccgccaacgt tccgacttcg
      541 gcagcccgtc gtaccggtac taccggcgac gggccgcggc ggtgcttgca gtgcgagtgg
      601 tcacccgcac ctgtttcccg ttgaaccccc ccggagaaac cggatggggc acccgagaac
      661 agcggagatt ctaccgcatc ggagtgggtt caaagccagg gaaagcggat cggaaagatc
      721 cccgggaggc cttgggtacc cccaatttcg gggtgggcgg agagcccgtg cggatatgtc
      781 aaagcacatt caaaaaaaac cgttagcagt tgacttaaca agatttttat cactatagtg
      841 tactcatgga agccgagctt aacgccctcg acgacaaagt tacccaactc gctcagttgt
      901 gccagaaact ccgcaaggac aacaaccagt tgcggcagca gctggtcacg gcacagagcg
      961 agagcaagcg cttggcggaa aaggtaaaca ccgccacgac ccgcctcgaa ggtttgctca
     1021 accagattcc cgatggagcc gaatgagcaa ggaagccagg gggctcgacg tgaacatcat
     1081 gggacgcgaa tttcgcgttt catgcacgga ggaagagcgg cgcgacctgc tcaaggcagt
     1141 cgactatctg aacgacaaga tgcgcgagat tcgcgacacc ggaaaagtca tcggcgtgga
     1201 acgcatcgcc atcatggcgg cgctgaacat cagccacgag tttctctcga cccacatgaa
     1261 tggcgggttt gacgtcggcg agttcaagcg tagaatgttg agcatgcagg caatcatcga
     1321 ccaggcgatg aacggccagg acgagttgtt ctgagggttt ttgcgacgat caatgtcccc
     1381 tgcggtgttg ttgggctcta cattctttga accaatgctc cttgccttgg ttgcggccta
     1441 aagcagcact gttgtgcgcg tcccagcggg aagcgcctga tgtgctcgga aagcgaccac
     1501 cttgaaccct aggttcagga tgccagcctg acggcaatcg cggggggcac ccccttccgg
     1561 atcagcgaca acaataggac ccgagcggca ctgccgcttt ttttgttttc acgcatgcac
     1621 cacaacgaga tagagttcca gcgcagcggg actctatttt tttgcctgtc gggatgatga
     1681 attactggct gatgaaatcc gagccggacg tattcggcat cgatcacctc gcggctgcgc
     1741 cggagcagac cgccgcctgg tggggcgtac gcaactacca ggcgcgcaat ttcatgcgcg
     1801 accggatgaa agtcggcgac cgcgcgttct tctatcactc cagctgcgca gagcccggcc
     1861 tcgccggcat cgtacgggtg tccagacctg cgcatccgga catgacgcag ttcgaccgca
     1921 agagcacgta cttcgatccg aaggccacac gggacagtcc acgctggttc aacgtcgacg
     1981 tcaccctggt gaaaaagacg cgcctggtcg gccttggaga actgcgttcc catccggggc
     2041 ttgcttcgat gcgggtgctg cagcgcggca accgcctgtc gatcaccccg gtagaggcgc
     2101 gcgagtggga tttcatcatg ggcattctct agaagcgcga cgaccgcaat ggaatggtgg
     2161 atcgtctatc tgctgatggg cctgtttgtc ggcttcttcg ccggcatgct cggggtcggc
     2221 ggtggattga tcctcgtcac actgatggtg tttctgttca gcctgcaggg atttcccgcg
     2281 gaccgcgtcg tgcacatcgc gctcggcacg tcgctgacca gcatcgtctt tacttcgatc
     2341 tccagcctgc gcgcgcatca ccgccacggc gcggtgcgct gggacatcgt caaaatggcc
     2401 acgcccgggc tggtcgcggg cacgctgctc ggcaccttcg tcgctgaccg gatggcatcg
     2461 aagtatctgg caatcttctt cgttgccttc gtgtactact ccgcggtgca gatgttcgcg
     2521 gacatgaggc ccaagccgac acgcactctt cccgggctgg cgggaatgtc ggtggcagca
     2581 atcatcactg gtatcatttc gagcctggtg ggcgccggcg gcggcttgct gtcgattccg
     2641 ctgatgacca tgtgcaacgt gccgatgcgc aacgcggtcg gcacctcggc cgcactgggg
     2701 ctgccgatcg cgatcgccgg cgcagcgggc tacatctata acgggctagg ccaggatcac
     2761 ctgccgccgc ttacgctcgg ctacgtttac gtcccggcgc tggtcgggat cgtaatcggc
     2821 actttcgtca ccgtcccggc gggcgcgaag gcagcgcaca ccatgccggt gccgcggctg
     2881 aaaaagattt tttccgtgat cctgttcctg atggcgacca agatgttgtg gacgctgttc
     2941 tgatatttac gatctggtct taaaccagcc tgcgaggctc agccgttcac gctttccggg
     3001 caggacttcg tgggcaaagc gctcgctcag aaaaagcacc agcgtcccgc cgaccggcag
     3061 tacgtccttg aattttcccg cttccccggc gtcgagatag agccgcagtt caccgccatc
     3121 ctcgcgcccc cagttctcgt tcagatagag cgcgcaggaa agccggcggc ggcggtcgcc
     3181 acgaaactgg tcgagatgtt tccggtagaa ggcgccctgc gcgtagcgcg agaaatggca
     3241 ttcgaactcg agcaacccga gctgcaactc gcggttgagc gccagccgca acgtctcgaa
     3301 gtgcgcaaga cagcggcgct gtggcgcagt cgcatccgct aagtcca
//
LOCUS       DASQOP010000164         6825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1678079, whole genome shotgun sequence.
ACCESSION   DASQOP010000164 DASQOP010000000
VERSION     DASQOP010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6825
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1678079"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VE030_06610"
     CDS             complement(<1..340)
                     /locus_tag="VE030_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23122.1"
                     /translation="MSKRPTPEFLRAVAAFRRYVHPERTEAWIERFAFGLADIEQVFE
                     AQVAGDPMLSPPRPPQYLGFEPGTYQPVSMSSDLFFAGVVQIAHQVRQRAMSALEIAT
                     LFLDRIDANRH"
     gene            complement(500..1615)
                     /locus_tag="VE030_06615"
     CDS             complement(500..1615)
                     /locus_tag="VE030_06615"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HZF23123.1"
                     /translation="MTQTFSCGHATSPNWRDAAAQCLQQAGKGPAGANLGFLYITDNF
                     AGEVAGILAYFRTNTGILHWTGTVGMGVVATGREYFDEPALAIMLCAFDDASFRVFSG
                     VRAPQDLERQPLHFGGLPANFAIVHGDPRNAQLGRLIGDFAGVMESGFIAGGLTSSRR
                     EVVQIADGIAKGGLSGVLFSDEVVVSTRLTQGCSPIGPRHTITGSRQNILISLDGRAA
                     LDVFREDVGELLSRNLTQVGGTIFAGLRVGGSGTGDYLVRNVVGIDPVHKLLAIGDVP
                     QAGGEIMFCRRDETSATEDMARMLDSIRSGLFGRPRGGLYYSCVARGPNLFGDESEEV
                     GMIRQALGDFPLVGFFCNGEISHNRLYGYTGVLTLFL"
     gene            complement(1615..2850)
                     /locus_tag="VE030_06620"
     CDS             complement(1615..2850)
                     /locus_tag="VE030_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HZF23124.1"
                     /translation="MDENHTNSAFANEQLRRRSLITAVRQRDAREAAKLLMSELPHVA
                     GQVLLELNPGQTSDILERVSEDFGARVLATVPTEYARQWARNQVYPHGSVSRMMDPAI
                     AVFPPHISVGHAIERLRSLIKTSFVTYGYITDENGKLVGLITMRDLLFAPHERTLAEI
                     MLEAPFSLGTEMQLADAMRPVLTRHYPVYPVCDERGTLVGLVRGSIMFEEQTLEISAQ
                     PATMVGVEEERLTTPWSQSLKFRHPWLQLNLLTAFLAAAVVGIFQDTLDKWVILALFL
                     PVLAGQSGNTGCQALAVTLRGITLGELRAGREAALVLKEASLGLLNGALVGITAGLGM
                     YIAARSQHNPQALLLGFIVFLAMIGSCVVSGISGAMIPLTLKRLGADPATASSIFLTT
                     ATDVASMGLFLGLATVLIR"
     gene            2981..3160
                     /locus_tag="VE030_06625"
                     /pseudo
     CDS             2981..3160
                     /locus_tag="VE030_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930982.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
     gene            3228..6305
                     /locus_tag="VE030_06630"
     CDS             3228..6305
                     /locus_tag="VE030_06630"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZF23125.1"
                     /translation="MTPQARAANQRPRERERLLLYLIPGLAALLVATLFTLAAFNLSK
                     ARQADLNDAGRATQNLAQVLEEQTARALQAVDVVLLNLADTWAQVPALRASSNPQMHA
                     VLQQKLATLTFARALFIVDSRGVITHHSDPLPAATQHFSDREYFRWHRTRPHGLYVGK
                     PIVSRTTGAWFVAASRRLPTADGSFGGVIVVALEPRAFQAFYRNVDVGHGGAIELLHQ
                     DGELIARTPAAPTWVGMATGSNLTLKDLARSGSLTFRTVSPVDGEARIYSARPVAGAP
                     LMVQVGLSEEEALAGWREQKSVTIIVLTGFTLAIAVLAWLLMQELRRRSELTRSAAQS
                     EGLLRQVIDILPVGVWVADGDGTVVLGNAASKKIWGQTPPAGTHQNGGLKGWRKDTGK
                     RLEWSDWGLARALAKGETTGDQVIDIESCDGSRKTILDSAAPILSTDGEVLGAISLSE
                     DITERRVLTETLCESEARYRALFGHSIDAILLTRPGGAILAANDAACRLLDYGEEELR
                     AIGRDDIVDGTDPRAQALLHAGDPAGAARGEVRLRRKDGALVPVEFACAPFSDRNGEA
                     CASLIIRDITDRKRAEEHIEYLAYHDELTGIPNRAYFHRAFEHALATTQRYGLRCALM
                     LVDLDRFKNINDTIGHEAGDQLLKQVAARLRACLRDSDIIARLGGDEFVILMQDASTV
                     DAVNAVALKILEATSRPLLIDDQEFLITASIGISTYPHDGADMQTLLKNSDVAMYRAK
                     ESGKNGFQYFSPEMNVHTRERLSIESALRRGLERKEFTVHFQPKVRVSSRTVAGMEAL
                     VRWRNPEKGLMQPSEFIPIAEETGIVVQIGDWVLEEACRQGQMLRESGHISLRVSVNL
                     SVRQLFDHGFAQRVADILEQTGFPAQYLELEITESMVMRDAERAVTLLQALRDTGVRL
                     AIDDFGTGYSSLAYLKRFPIDCVKIDRSFIRDLPEDRDDASITRSIIAMAHNMKLDVV
                     AEGVETPEQLEFLHAYGCDEIQGFLFSEPLAADAFERFLGAPAQHAPATYLHA"
     gene            6365..>6825
                     /locus_tag="VE030_06635"
     CDS             6365..>6825
                     /locus_tag="VE030_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZF23126.1"
                     /translation="MSAVLQASNEPVLVRNDAGGIATVTLNRPKQYNALSEELLTELQ
                     SALDSIEKDPSVRVVVIAGNGAAFCAGHDLKQMRANPKKAYYKKLFAQCSEMMMTLTR
                     LPQPVIARVHGIATAAGCQLVAQCDLAVASDNARFAVSGINVALFCSTPSVP"
BASE COUNT         1282 a         2232 c         2182 g         1129 t
ORIGIN      
        1 gatgccggtt ggcatcgatg cggtcgagaa acaaggtggc gatttccagc gccgacattg
       61 cccgctgcct gacctgatga gcgatctgca ccacaccggc aaagaacagg tccgaggaca
      121 tcgacaccgg ctggtaggta ccgggctcga agccgaggta ctggggaggg cgcggcgggg
      181 aaagcatcgg atcgcccgcg acctgcgcct cgaacacctg ctcgatatct gccagcccga
      241 aagcgaagcg ctctatccag gcttcggtgc gttcaggatg gacataacgg cgaaatgccg
      301 cgacggcacg cagaaactcg ggagtggggc gttttgacat gggaatccgt gactatcgtg
      361 acgacgtgac tatcgtgact cgtaatctat caagatgttt tgcgaggtgg aatcatcttg
      421 cttccagcgt acaccgaaac gcgctttccg cgcttttgcg atagtcacga tagtcacgtc
      481 gtcacgccag tcacgtcttc tacaaaaaca acgtgagaac gccggtatac ccatacagcc
      541 tgttgtggga aatctcgccg ttgcagaaaa atccaaccag cggaaaatca ccgagcgcct
      601 gacggatcat gccgacctcc tcggactcgt cgccgaagag attcggaccg cgggctacac
      661 aggaataata caggccgccg cgcggacgcc cgaacagccc gctcctgatg ctgtccagca
      721 tgcgcgccat gtcctcggtt gcgctggttt cgtcgcggcg gcagaacatg atctcgcccc
      781 cggcctgcgg cacgtcgccg atcgccagca gcttgtgcac cgggtcgatg cccacgacat
      841 tgcgcaccag gtagtcgccg gtgccggagc cgccgacgcg cagcccggca aaaatggtgc
      901 cgccgacctg cgtcaggttg cgcgacagca gctcgcctac atcctccctg aaaacatcga
      961 gcgctgcgcg cccgtcgagc gagatcagga tgttctggcg gctgccggtg atagtgtgac
     1021 ggggaccgat cggcgaacaa ccctgggtaa gccgcgtgga caccacgacc tcatcggaga
     1081 acagcacgcc ggaaaggccg cctttggcaa tgccgtcggc aatctgcacc acttcgcggc
     1141 gcgagctggt cagccctccg gcaatgaatc cgctttccat gactccggcg aagtcgccga
     1201 tcagacgccc cagctgggcg ttgcgcgggt cgccgtggac gatggcgaag ttggccggca
     1261 gcccgccgaa gtgcagcggc tgccgctcca ggtcctgggg cgcgcgcacc ccggagaaga
     1321 cccggaagct tgcgtcgtcg aacgcgcaca gcatgatcgc cagcgccggc tcgtcgaagt
     1381 actcgcgccc agtggcaacc acgcccattc ccacggtgcc ggtccagtgc aggatgccgg
     1441 tattggtgcg gaagtaggcc agaatgcccg cgacttcgcc ggcgaagttg tcggtgatgt
     1501 aaagaaagcc gaggttcgcc cccgccggtc ccttgccggc ctgctgcagg cactgcgcgg
     1561 ccgcatcacg ccagttgggc gaagtagcgt ggccgcagga aaaagtttgg gtcatcagcg
     1621 gatgagcacg gtcgccagcc cgaggaacag gcccatgctg gcgacgtcgg ttgcagtcgt
     1681 gagaaagatg ctggaggccg tggcggggtc ggcccccagc cgcttcagcg tcagcgggat
     1741 catggcgccg gagatgccgc ttaccacgca actgccgatc atcgccagaa agacgatgaa
     1801 gccgagcagc agtgcctgcg ggttgtgctg tgagcgcgcg gcgatataca tgcccagccc
     1861 ggcggtgatg ccgaccagcg cgccattgag cagtccgagc gaagcctctt ttagcaccag
     1921 cgcagcctcc ctgccggcgc gcaactctcc gagtgtaatg ccgcgcaacg tcacggccag
     1981 agcctgacag ccggtattgc ccgactgtcc ggccagcacc ggcaggaaca gcgccaggat
     2041 cacccatttg tcgagcgtgt cctggaagat gccgaccacg gcagcggcca ggaatgcagt
     2101 cagcagattc aattgcagcc acgggtggcg gaatttcagg ctttgtgacc agggcgtggt
     2161 gagccgctcc tcctcgacgc ccaccatggt ggccggctgg gcgctgatct ccagggtctg
     2221 ctcctcgaac atgatcgatc cgcgcaccag accgaccagt gtgccccgtt cgtcgcacac
     2281 cggataaacc gggtaatggc gggtcagtac cggcctcatg gcgtccgcga gttgcatttc
     2341 tgtacccagg ctgaacggcg cctcgagcat gatctcggcc agggtgcgct cgtgcggagc
     2401 gaacagcagg tcgcgcatgg tgatcagccc caccagtttg ccgttctcgt cggtgatgta
     2461 gccataggtg acgaacgaag tcttgatcag gctgcgcagc cgctcgatgg catggccgac
     2521 gctgatgtgc ggcgggaaca ccgcgatcgc cggatccatc atgcggctca cgctgccatg
     2581 cgggtaaacc tggttacgcg cccactggcg ggcatactcg gtcgggacgg tggccagaac
     2641 ccgcgcaccg aaatcctccg acacgcgctc caggatgtcg ctggtctgcc ccggattgag
     2701 ctcgagcagc acctgtcccg cgacgtgggg cagttcgctc atcagcagct tcgcggcttc
     2761 gcgcgcatcg cgctgcctga ccgcggtgat caacgaccgg cggcgcagct gctcgttggc
     2821 gaatgcagag ttggtatggt tttcgtccat cggccgcacc tgtctgggaa tatccgaagt
     2881 gcttcgagtt taccgcgggc cgctcgccgg gggcgctagg ccggtgcaga gtgcggcggt
     2941 atccgtatag ccagcgtcct tttactgcgg aaatccggcc atgaatctcg accgcgtcac
     3001 atcagggcgg agtctacccg acgacttcaa tgtgatcatc gaaattccaa tgaacgcaga
     3061 cccgatcaag tacgaggtgg acaaggatac cggcgcgctg ttcgtggacc gcttcatgat
     3121 gaccgccatg cactacccct acaattacgg ctacatcccc ctcaaattgg gttccctgca
     3181 gggcaaggtc gcggtacact ctgcgccgtg actgtcaccc gatcgccgtg acgccgcagg
     3241 cccgcgctgc caaccagcgc ccgcgagagc gggaacgcct gctgctctac ctgatcccag
     3301 ggctcgccgc cctgctggtc gccaccctct tcacccttgc tgctttcaat ctgtcgaaag
     3361 cgcgccaggc cgatctgaat gacgccgggc gcgccaccca gaacctggcc caggtgctgg
     3421 aagaacagac cgcgcgcgcg ttgcaggcgg tggacgtggt gctgctcaac cttgccgaca
     3481 cgtgggcgca ggtaccggcg ctgcgcgcaa gcagcaaccc gcagatgcac gccgtgctgc
     3541 agcagaagct cgccacgctt acctttgccc gcgccctgtt catcgtcgat tcccgcggcg
     3601 tgatcacgca ccactccgac ccgctcccgg ccgcgaccca gcacttttcc gatcgcgagt
     3661 atttcaggtg gcaccgcacg cggccacacg gactttatgt cggcaagccg atcgtcagcc
     3721 gcaccacggg ggcgtggttc gtcgcggcaa gccgccgttt gcccactgcg gacggcagtt
     3781 tcggcggcgt gatcgtggtg gcgctggagc cgcgcgcgtt ccaggccttt taccgaaatg
     3841 tagacgtcgg ccacggcggc gccatcgagt tgctgcatca ggatggcgag ttgatcgccc
     3901 gcactccggc cgctcccacc tgggtaggca tggctaccgg cagcaacctg acgctgaagg
     3961 acctcgcccg ctctggcagc ctcaccttcc gcacggtaag cccggtggac ggcgaggcac
     4021 gcatctacag cgcgcgcccg gtggcgggcg ccccgctgat ggtgcaggtg ggactgtcag
     4081 aagaggaggc gctggccggc tggcgcgagc agaagagcgt gaccatcatc gtgctgaccg
     4141 gctttaccct ggccatcgcg gtgctcgcct ggctgctgat gcaggagctg cggcgcagga
     4201 gcgagctgac gcgctcggct gcgcaaagcg aagggctgct gcgccaggta atcgacatcc
     4261 tgccggtcgg cgtgtgggtc gccgacggcg atggcactgt ggtgctgggc aatgccgcaa
     4321 gcaaaaaaat ctggggacag acgcctccgg cgggcacgca ccaaaacggc gggctgaagg
     4381 gctggcgcaa ggatacgggc aagcgcctgg agtggtccga ctggggcctg gcacgagcac
     4441 tcgccaaggg ggaaacgacc ggagaccagg tcatcgacat cgagtcctgc gacggcagcc
     4501 gcaagaccat cctcgattct gccgcgccga ttctgtccac ggacggcgag gttcttggcg
     4561 ccatatcgct gagcgaagac atcaccgaac ggcgcgtgct gacggaaacg ttgtgcgaga
     4621 gcgaagctcg ctaccgggca ctgttcgggc atagcatcga cgcgattctc ctgacccggc
     4681 ctggcggcgc catcctggcc gccaacgacg cggcctgccg gctgctggac tatggcgagg
     4741 aggaactgcg tgcaattggt cgcgacgaca tcgtggacgg gaccgatccc cgggcgcagg
     4801 cgctgctgca cgcgggggac cccgccggcg cggcgcgcgg cgaagtgagg ctgcgccgca
     4861 aggatggcgc cctggtaccg gtggagttcg cctgcgcccc cttcagtgac cgcaacggcg
     4921 aggcgtgtgc cagcctgatc atccgcgaca tcaccgaccg caagcgggcc gaagagcata
     4981 tcgaatatct tgcctaccac gacgaactga ccggcattcc caaccgcgcc tacttccacc
     5041 gggcctttga gcacgctctc gccaccaccc agcgttacgg attgcgctgc gcgctgatgc
     5101 tggtggacct ggaccgcttc aagaacataa acgacaccat cggccacgaa gccggcgacc
     5161 agttgctcaa gcaggtggcg gcacggctgc gcgcgtgcct gcgcgacagc gacatcatcg
     5221 ccaggctcgg cggcgacgag ttcgtgatcc tgatgcagga cgcctccacc gtcgacgccg
     5281 taaacgcggt agccctcaag atcctggagg ccacctcccg gcccctgctg atcgacgacc
     5341 aggagttcct gatcaccgcc agcatcggca tcagcaccta tccgcacgat ggagccgaca
     5401 tgcagacgct gctcaagaac agcgacgtgg cgatgtaccg cgccaaggag tcaggcaaga
     5461 acgggttcca gtacttctcg ccggaaatga acgtgcatac ccgcgagcgg ctgtcgatcg
     5521 agtcggcgtt gcggcggggg ctggagcgca aggagttcac ggtgcacttc cagcccaagg
     5581 tgcgggtttc ctcgcgcacg gtggcgggca tggaagcgct ggtgcgctgg cgcaacccgg
     5641 aaaagggcct gatgcagccg agcgaattca tcccgatcgc ggaggagacc ggaatcgtcg
     5701 tgcagatcgg tgactgggtg ctggaagagg cctgccgcca gggccagatg ctgcgcgagt
     5761 ccgggcacat cagcctgcgc gtgtcggtga acctgtccgt gcggcaattg ttcgaccacg
     5821 gcttcgcgca gcgcgttgcc gacattctcg agcagaccgg gtttccggcg cagtatctcg
     5881 agctggaaat caccgagagc atggtaatgc gcgacgccga gcgcgcagtg acacttctgc
     5941 aggccctgcg tgacaccggg gtgcgcctgg cgatcgacga cttcggcacc ggctactcgt
     6001 cgctcgccta cctgaagcgc ttcccgatcg actgcgtgaa gatcgaccgc tccttcatcc
     6061 gcgacctgcc ggaagaccgc gacgacgcct cgatcacccg cagcatcatc gccatggcgc
     6121 acaacatgaa actcgacgtg gtcgcggaag gcgtggaaac gccggagcag ctcgaattcc
     6181 tgcacgccta cggctgcgac gagatccagg gcttcctgtt cagcgagccg ctcgcggcgg
     6241 atgcattcga gcgtttcctc ggcgctccgg cccagcacgc gcctgcgacc tacctgcacg
     6301 cctagaaaac cccgctgctg gcgcctataa ttcatgctcc gggaggggca cagcggagaa
     6361 ttccatgtct gccgtactgc aagcgagcaa tgaaccggtg ctagtgcgca atgacgcggg
     6421 cggaatcgcc accgtcacgc tcaaccgtcc gaaacagtac aacgcgctct ccgaagaact
     6481 gctgaccgaa ctgcagtccg cactcgattc gattgaaaag gacccatcgg tacgtgtggt
     6541 ggtcattgcc ggcaacggcg cagctttctg cgccggtcat gacctcaagc aaatgcgcgc
     6601 caacccgaaa aaagcgtatt acaagaagct tttcgcccag tgcagcgaga tgatgatgac
     6661 cctcacccgt ttgccgcagc cggtgatcgc ccgggtgcac ggcatcgcca ccgccgccgg
     6721 ctgccagttg gtggcgcagt gcgacctggc ggtggcctcg gacaatgcgc gcttcgccgt
     6781 gtccggaatc aatgtcgccc tgttctgctc gacgcccagc gtgcc
//
LOCUS       DASQOP010000165        10871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_68418, whole genome shotgun sequence.
ACCESSION   DASQOP010000165 DASQOP010000000
VERSION     DASQOP010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10871
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68418"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(236..1522)
                     /locus_tag="VE030_06640"
     CDS             complement(236..1522)
                     /locus_tag="VE030_06640"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZF23127.1"
                     /translation="MNDATPSKIGKYPVIREIGRGATSRVYLGRDPFANRDVAIKLVS
                     TEAGVDTDLRRRFRKVFMNEASLVGKLIHPHIMAIYDADVADAYSYIVMEYVDGPTLE
                     QYCNVASLLPVERVVEVAFKCSLALEFAHRHGVIHRDIKPANILISAQSDIKISDFGV
                     ALQAEAEHTHLQGVGSPSYMSPEQVQDKELSHQTDIYSLGVVMYQLLTGKLPFIAATK
                     ASLLYQIINIEPLPPSTHRTDISPDLDRVVMRALNKDVRNRYQTWGDFSRDLTQTYRH
                     LKLPPESISDTEKFNAIKALPFFRDFRDVEIWETVRIASFRRIQPGKTVIREGDVGES
                     FFIIATGEARVAKAGNLLDTLRAGDCFGEILYFEDTRARRITTISSTTALALIEIKAA
                     ALQKATDACQKQFNQAFLRILIDRLTWANSRLSARS"
     gene            complement(1558..2166)
                     /locus_tag="VE030_06645"
                     /pseudo
     CDS             complement(1558..2166)
                     /locus_tag="VE030_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069601.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
     gene            2453..2650
                     /locus_tag="VE030_06650"
     CDS             2453..2650
                     /locus_tag="VE030_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HZF23128.1"
                     /translation="MLKWLLTLVIALLLLGVLSPWLRKLGFGRLPGDIDIQRNGRRYS
                     FPVGSTVMLSLLASLIYWMLR"
     gene            complement(2700..3161)
                     /locus_tag="VE030_06655"
     CDS             complement(2700..3161)
                     /locus_tag="VE030_06655"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HZF23129.1"
                     /translation="MAASKLTIALAAAVALFVPHLAISADEHGSRMEARFKAADKDND
                     GSLTREEAKAMPRVAKNFDAIDADKSGTVTLAEIQAAMKKMVKEAHDRNVEHFKAADK
                     DNDGSLTREEAKALPRVAKNFDAIDTDKSGTVTLKEIRAYMKAHRQERKRS"
     gene            complement(3211..4434)
                     /locus_tag="VE030_06660"
     CDS             complement(3211..4434)
                     /locus_tag="VE030_06660"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HZF23130.1"
                     /translation="MKIYAVGGAVRDRLLGREVADQDYVVVGATPEQMEQLGYRPVGK
                     DFPVFLHPQTHEEYALARTERKTARGYKGFDVYAAPEVTLEQDLARRDLTINAIARDE
                     KGNIIDPFHGVADLKAGILRHVSPAFVEDPVRVLRVARFAARFGFTVAPETLELMREM
                     VRNGEVDHLVAERAWLEISRGLMEATPSRMFLTLRECGALARILPEVDALFGVPQPPE
                     HHPEVDTGVHIMLALDYAAANKFPLPVRFAALTHDLGKGVTPKALWPKHHGHEHKSVE
                     LAGRLCQRLRAPNECRDLAVLVARYHGDAHRAEELRPATILKLLSATDVYRKPERFEE
                     FVHACACDFHGRTGNERRPYPAADILRRAAAAARAVDAGAIALGVGDPGRISAAIEAA
                     RLKAVTEALAATRQR"
     gene            complement(4431..5084)
                     /locus_tag="VE030_06665"
     CDS             complement(4431..5084)
                     /locus_tag="VE030_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HZF23131.1"
                     /translation="MSNPTLVIGNKAYSSWSLRPWLLMKHAGIAFDEVRIPLYRQDSK
                     ETILRYSPAGRVPVLVDGSLTVWDSLAICEYLAEKHPEKRLWPADTRARANARSISAE
                     MHSGFANLRGQMAMNVRRRMPGRGRTPEVLAEISRIQAIWNDCRARFGAAGPFLYGGF
                     SAADAMYAPVVSRFHTYEVELKGGAREYAEFMYALAPMQEWITGANAEAEVISEYEP"
     gene            complement(5132..6082)
                     /locus_tag="VE030_06670"
     CDS             complement(5132..6082)
                     /locus_tag="VE030_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HZF23132.1"
                     /translation="MRVNSVCVIGGSGFLGRHIAHQLADFGIRVHLPTRHRERAKQLI
                     VLPTVDVITADVNDPATLSRLVAGNDAVVNLAGILHERRKGDFARVHTELPRKIVNAC
                     REQGVRRLLHVSALGAAHDAPSEYLRSKAGGEQQIRVAQAAGIRTTIFRPSVIFGRED
                     SFLNLFARLVRSLPVIVLASPQARFQPVFVEDVAHAVVLSLGEARTADQSYELCGPGI
                     FTLQELVEYVCRVLGVERPIIPLNDPLSYLQAWMMEWLPVKLMTRDNYYSMKVDSVCA
                     CPFPRVFGFEPTPLEAVVPLYLAENTPRARYRWFRFRARR"
     gene            complement(6152..8056)
                     /locus_tag="VE030_06675"
     CDS             complement(6152..8056)
                     /locus_tag="VE030_06675"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HZF23133.1"
                     /translation="MKKSLAVLLFASCAALAAPDDEVFLEAREAFQRGDIVRLDRLAK
                     QLDDNYPLALYVQYWQLRSRLADLSAGEIENFLARHNNSLAADRLRADWLRQLAKDRD
                     WPGFLREYPQLTTEDLELVCFAYQARLALGESGILTQARPLWFTAQVLPESCLPLFEL
                     MFKKDMLTAEDSWTRIRLALEIGNLSGARMLLGYLPAGQRPDAKTLDSIARRPQHFLD
                     RAPLPLKTRAQRELVIYALYKTAEGWPQTAADRLHRIAKDLPDTEREYAWAQIASVAA
                     RSLHPDALTWFRNAGNGMNDRQLTWRARSALRAGDWNEVFAAIEAMSPTEYKYAQWRY
                     WKARALSALGRQSEANALLAPLSNQFNFYGQLAAEELGTSISTVPQTYRPDEDEIEAT
                     AKDPGIVRALTLYAIGLRYEGALEWQWAVKDYEDKRLLAAAELASRNEWYERTIDTAE
                     RTVLLHDFGLRYPTPYREVVQDYASQLDLDEAWVYGLVRQESRFAHTARSTAGASGLM
                     QLMPSTARWVAKRLGLSGHHAKLTATADSNIVLGTYYLRQMMDSLDNQMVLASAGYNA
                     GPRRAREWIAGRSLEGAVYIETIPFAETREYVKRVMNNTLYYARLLHQAGPGLRERLG
                     TVPARMPQNN"
     gene            complement(8134..9645)
                     /gene="ilvA"
                     /locus_tag="VE030_06680"
     CDS             complement(8134..9645)
                     /gene="ilvA"
                     /locus_tag="VE030_06680"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HZF23134.1"
                     /translation="MKIDYLERILTARVYDVAVETPLELAPALSGRLGNTLLLKREDM
                     QPVFSFKLRGAYNKMANLSAAQRRRGVIAASAGNHAQGVALAANHLRCRATIVMPATT
                     PQIKIAAVAARGARVVLFGDSYDEAYAHSLKIARREKLTFVHPYDDPDVIAGQGTIGM
                     EILRQHGGPIDAIFVAVGGGGLIAGIAAYVKRLRPEIRIIGVEPVDADAMTRSLAAGH
                     RVRLAQVGLFADGVAVKQVGSETFRLCRELVDEMILVDNDAVCAAIKDVFEDTRSILE
                     PAGALAIAGAKAYAERHKHKGRTFVAVACGANMNFDRLRFVAERAELGEKREAILAVT
                     IPERPGSFKQFCSLLGARNVTEFNYRYADPVEAHVFVGVQVRNRDETRKLIARLRRKG
                     LPTLDFSDNEMAKLHVRHLVGGRAPSVENERLYRFEFPERPGALMNFLDSMSHDWNIS
                     LFHYRNHGADYGRVLVGMQVPPRDQAAFRRFLSQLGYAHSDETRNPAYSLFLA"
     gene            9768..10427
                     /gene="rpiA"
                     /locus_tag="VE030_06685"
     CDS             9768..10427
                     /gene="rpiA"
                     /locus_tag="VE030_06685"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HZF23135.1"
                     /translation="MTQDELKKAVAKAAIAHVPEDCIVGVGTGSTANFFIDELARIKG
                     RLEGAVASSEASAARLKVAGIRVFDLNSVDELPVYVDGADEITRHLHMIKGGGGALTR
                     EKIVAAVASKFVCIADEAKLKDVLGAFPLPVEVIPMARSYVARELVRLGGHPELRQGF
                     TTDNGNIILDVRGLAIVNPVALEGEINQIVGVVANGLFARRGADVLLLGTRDGVKTLT
                     R"
     gene            10500..>10871
                     /locus_tag="VE030_06690"
     CDS             10500..>10871
                     /locus_tag="VE030_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HZF23136.1"
                     /translation="MTNSEHTSKQFDAELEAVRARVLHMGGLVEEQIKLALDALISGD
                     LELCTKVENNDHMVNGLEVGIDEDCSTIIARRHPAAGDLRMLMTVVKTITDLERIGDE
                     AAKIARMAKLIYSADRLQQPRT"
BASE COUNT         1915 a         3367 c         3499 g         2090 t
ORIGIN      
        1 gcgaccaacg ttgtcgaagt catgaatgcc tgcatcagat cgttcgtggc aaaaaaggcg
       61 ccattgtagc gttttgcccg ctccgggcgc attttcaagc gcgtactccg gaggaaacct
      121 gcaagttttg aatcacacag aaatccttgc ctcgtttttt ctctgtgccc tctgtgttct
      181 ctgtggcgca aggttttgat ttttctttca gtcaaccggt gtttcgcatt gcctgctaac
      241 ttcttgccga gagccggctg ttcgcccagg tcagccggtc gatgaggatg cgcagaaacg
      301 cctgattgaa ctgcttctgg caggcgtcgg tggctttctg taacgccgcg gccttgatct
      361 cgatgagcgc gagagccgtg gtcgaggaaa tcgtggtgat ccgccgcgcg cgggtatcct
      421 cgaaatagag tatttctccg aagcaatcgc cggcacgcag cgtgtcgagc aggttccccg
      481 ccttggctac ccgcgcctcg ccggtggcta tgatgaaaaa actctcgccg acatcgccct
      541 cgcggattac cgtcttgccg ggctggatgc ggcggaagga ggcaatccgc accgtctccc
      601 agatctccac gtcgcggaaa tcccggaaaa agggcagcgc cttgatggca ttgaatttct
      661 cggtgtctga aatcgactcc ggcggcaatt tcaggtggcg gtaagtctgg gtaagatcgc
      721 ggctgaagtc tccccaggtc tggtagcggt ttcttacgtc cttgttgagc gcgcgcatca
      781 ccacgcggtc gaggtcggga gagatatcgg tgcgatgcgt tgaaggcggc agcggctcga
      841 tgttgatgat ctggtagagc aggctcgcct ttgtggcggc gatgaatggc agcttgccgg
      901 tcaacaactg gtacatcacc acgcccagcg agtagatgtc ggtttggtgc gacaactcct
      961 tgtcctgcac ttgctcggga gacatgtacg acggtgaacc gacgccctga aggtgagtgt
     1021 gctcggcttc cgcctgcaat gccacgccga aatcgctgat cttgatgtca ctctgcgcgc
     1081 tgatcaagat gtttgccggc ttgatgtcgc ggtggatcac cccatgccgg tgtgcgaatt
     1141 ccagtgccag gctgcacttg aacgcgactt ccaccacccg ctccaccggc aggagactcg
     1201 ctacgttgca atactgttcc aaagtcggtc catccacgta ctccatgacg atatagctgt
     1261 aagcgtctgc cacgtcggca tcgtagatgg ccatgatgtg cggatggatc agtttgccga
     1321 ccagcgaggc ctcgttcatg aataccttgc gaaagcgtcg ccgcaaatcc gtatccacgc
     1381 cggcctcggt gctcaccagc ttgatcgcaa cgtcccggtt ggcaaacgga tcgcggccca
     1441 gatagacccg gctggtcgcg ccgcgcccga tctcccggat caccggatac ttgcctatct
     1501 tggagggagt cgcgtcgttc atggattcat gacggcgacg tgtcagcgtg gtgccgggac
     1561 tttcatgccg cgctgcacag ccgggcgtgc cccgatcgtc tcataccagc gcttgacgtt
     1621 cggaaactcg gccagctcga ccttgtgcca gtcatggcgg gcgacccacg gataggtggc
     1681 gatgtcggcc accgaataat ccgcggcgag ccaatcgcca gcggccagac gctggtccag
     1741 taccgcatac agtctgcgtg tttccttggt gtagcgcgcg atcgcgtacg gaacgggttc
     1801 cggcgcggcg cgcaggaagt gatgggtctg gccgaacatc gggccgaccc cgcccatctg
     1861 gaacatcagc cactgaatgg cgtcgaagcg ctcggccgtt cgcgtggaca ggaatcggcc
     1921 ggtcttttcg gccaggtaga gcaggatcgc gccggactcg aacagcgcat aaggcttggc
     1981 ctccggcccc tcgctgtcca cgatcgccgg aatcttgctg ttgggactca tggctacgaa
     2041 ttccggcctg aactgatcgc ctttgccgat atcgacagcg tgtactgcgt agggcaaatc
     2101 caattcttcg agcatgatgg aaaccttgcg gccgtttggc gtgctccacg tgtacaaatc
     2161 gatcaacgcg cgctcctggt gtattgcggg gaagggtgcc gattttatag gaaagcagga
     2221 accgcgcccg tataataggg actcggctgc ttgccacgaa gacgcgaata acacgaagaa
     2281 aacgagttct tggtgcagac atgggcttca ggccatgtcc tggccggtat gccccaaaca
     2341 ggaatttctt ctcgcttctt tgcgtattcg cttcgggagg tccggttttt gtctggccgg
     2401 tcttcgtgtc ttcgcgggat aaatttgtgt tgccggacga acggagagcg aagtgctgaa
     2461 atggctgctg acgttggtca tcgcgctgtt gctgctgggc gtgctgtctc cctggctgcg
     2521 caaactcggt ttcgggcgcc tgcccggcga catcgatatc cagcgcaacg gccggcgcta
     2581 ctcgtttccc gtcggctcca cggtcatgct gtcgctgctg gcgagcctga tctactggat
     2641 gctgagatga aaaaacggac aggcgaacct gtccgttttt ggtacggctg tcgccgcctt
     2701 cagctcctct tgcgttcctg gcggtgcgcc ttcatgtagg cgcgaatttc cttcagggtg
     2761 accgtgccgc tcttgtcggt gtcgatcgca tcgaagttct tcgccactct gggcagcgcc
     2821 ttggcttcct cgcgcgtcag gctgccgtcg ttatccttgt cagctgcctt gaagtgctcc
     2881 acgttgcggt cgtgcgcttc cttgaccatt tttttcatcg ccgcctgaat ttcggccagc
     2941 gtgaccgtgc cgctcttatc ggcgtcgatg gcgtcgaagt tctttgccac ccgcggcatc
     3001 gccttggctt cctcgcgcgt caggctgccg tcgttgtcct tatcggccgc tttgaagcgt
     3061 gcctccatgc gggagccgtg ttcgtccgcc gaaatggcaa gatgggggac gaaaagcgcg
     3121 actgcagctg cgagcgcaat cgtcaacttg gatgcagcca tatgagcctc cattggtatt
     3181 gatgaccccg caacaacggc tcgtgccggt ttagcgttga cgcgtggccg ccagcgcttc
     3241 ggtgacggcc ttcaggcggg cggcctcgat tgcagcggaa atccggcccg ggtctccgac
     3301 tccaagggcg attgcgccgg cgtccaccgc gcgcgcagcg gcggcggcgc gtcgcagaat
     3361 atccgcagca ggatatgggc gccgctcatt accggttcgg ccatgaaagt cgcaggcgca
     3421 ggcgtggacg aattcctcga accgttccgg tttgcggtat acgtcggttg cggaaagcag
     3481 cttcagtatc gtggccgggc gcagttcttc ggcgcggtgc gcgtcgccgt ggtagcgcgc
     3541 caccagcacc gccaggtcgc ggcattcgtt gggcgcgcgc agccgctggc acaatctccc
     3601 ggccagctcg acgctcttgt gctcgtggcc atggtgcttc ggccacagcg ccttcggagt
     3661 cacgcccttg cccagatcgt gggtgagcgc ggcaaaacgc accggcagcg ggaatttatt
     3721 ggcggcggcg taatccagcg ccagcatgat gtgcaccccg gtgtccactt ccgggtgatg
     3781 ttccggcggc tgcggcacgc cgaatagcgc gtcgacttcg ggaaggattc gcgccagcgc
     3841 cccgcactcg cgcagcgtga ggaacatgcg cgagggcgtg gcttccatca gtccgcggga
     3901 gatttccagc caggcgcgtt ccgcaaccaa gtgatccact tcgccgttgc ggaccatctc
     3961 ccgcatcagt tccagcgttt ccggcgcgac agtgaatccg aagcgggcgg cgaaacgcgc
     4021 cacgcgcagc acccttaccg gatcctcgac gaaggccggg ctgacgtggc gcagtatgcc
     4081 ggccttgagg tcggccacgc cgtgaaaggg gtcgatgatg ttgcctttct catcgcgggc
     4141 gatcgcattg atggtgagat cgcggcgcgc cagatcctgc tccagcgtga cctccggggc
     4201 agcgtacaca tcgaagccct tgtagccgcg cgcggtcttc ctttcggttc gtgccagcgc
     4261 gtattcctcg tgggtctgcg gatgcaggaa aaccgggaaa tccttgccca ccggccgata
     4321 gcccaactgt tccatttgtt cgggcgttgc gccgaccacg acgtagtcct ggtccgccac
     4381 ctcgcgtccg agcagacggt cacgcaccgc accgccgacg gcgtagatct tcatggttcg
     4441 tattcggaga tgacttcggc ctcggcattc gccccggtga tccactcctg cattggcgca
     4501 agcgcgtaca tgaactcggc atactcgcgc gcgccgcctt tcagttccac ttcgtaagtg
     4561 tgaaagcgcg acaccaccgg ggcatacatg gcgtcggccg ctgaaaaccc gccgtagaga
     4621 aacggcccgg cggcgccgaa gcgcgcgcgg cagtcgttcc atatcgcctg gatgcgcgaa
     4681 atctctgcca gcacctcggg cgttctgccg cgccccggca tccggcgccg cacgttcatc
     4741 gccatctgac cgcgcaggtt ggcgaatccg gaatgcattt cggcgctgat cgagcgtgcg
     4801 ttggcccgtg cccgggtgtc cgccggccag agccgcttct ccggatgctt ctcggccaga
     4861 tattcgcaga tcgccagcga gtcccacacc gtcaagctgc cgtccaccaa cacgggtacg
     4921 cggcctgctg gcgagtaacg caggatggtt tccttgctgt cttggcgata gagcggaatg
     4981 cgaacttcgt cgaaggcgat tccggcgtgc ttcatcagca accacgggcg cagcgaccag
     5041 gaggaatagg ccttgttgcc gattaccagg gtgggattgg acatcgaaca actcctgaag
     5101 gacgcacggg gcgggatggc gcggtttgaa atcaacggcg ggcgcggaaa cggaaccacc
     5161 ggtaccgcgc cctcggagta ttctcggcga gataaagcgg caccaccgcc tcgagcggcg
     5221 tcggttcgaa accgaacacg cggggaaagg ggcaggcaca gacgctgtcc actttcatcg
     5281 agtaataatt gtcccgcgtc atcagcttca ccgggagcca ctccatcatc caggcctgca
     5341 ggtaggaaag cggatcgttg agcgggatga tcggccgctc cactccgagc acgcggcaga
     5401 cgtactcgac caactcctgc aaggtgaaaa tccccgggcc gcaaagctcg taactttggt
     5461 cggcggtgcg cgcttccccg aggctcagca ccacggcgtg cgccacgtcc tcgacgaaca
     5521 ccggctggaa gcgtgcctgc gggcttgcca gcacgatcac cggcaagctg cgcaccaggc
     5581 gcgcgaacag gttgagaaag ctgtcctcgc gcccgaagat caccgagggg cggaagatcg
     5641 tggtgcggat gccggcggcc tgcgccacgc ggatctgctg ctcgccgccg gccttcgagc
     5701 gcaggtattc gctgggggca tcgtgcgctg ccccgagcgc ggaaacgtgc agcagccggc
     5761 gcacgccctg ctcgcggcag gcattcacga tcttccgcgg cagttcggtg tggacgcgcg
     5821 cgaaatcgcc cttgcgcctc tcgtgcagga tgccggccag gttcaccacc gcgtcgttgc
     5881 ctgcgaccag gcgcgaaagc gtcgccggat cgttgacgtc ggcagtgatc acgtcgaccg
     5941 tgggcagaac gatgagctgt ttggcgcgct cgcgatggcg ggtgggtagg tgcacccgga
     6001 ttccgaagtc ggcgagctga tgggcgatat ggcggcccag aaaacccgag cctccgatga
     6061 cgcagaccga gttgactctc atgccttcga tacgaagaat tttttccgcg atggccgcgg
     6121 ggcgaaaagt catggggcac agagccgcgg ctcagttgtt ctgcggcatc ctggccggga
     6181 cggtgccgag ccgctcgcgc agaccgggtc ccgcctggtg caaaagccgc gcatagtaca
     6241 gcgtattgtt catgacccgc ttgacgtact cgcgcgtttc ggcgaacggt atggtctcaa
     6301 tataaaccgc accctccaga gagcggccgg ctatccattc ccgcgcgcgg cgcgggccgg
     6361 cgttgtagcc ggcggaggca agcaccattt ggttgtccag ggagtccatc atctggcgca
     6421 ggtagtaggt gcccagcacg atgttggagt ccgccgttgc ggtgagcttg gcgtgatggc
     6481 cgctcaagcc caggcgcttc gccacccagc gtgcggtcga cggcatcaac tgcatcaatc
     6541 cgctggcgcc ggcagtggat cgggcggtgt gcgcgaagcg gctctcctgg cgcaccaggc
     6601 cgtagaccca ggcctcgtcg aggtcgagct ggctggcgta atcctgcacg acctcgcgat
     6661 acggggtggg gtagcgcaat ccgaagtcgt gcagcaacac ggtgcgttcc gcggtatcga
     6721 tagtgcgctc ataccattcg ttgcgtgatg ccagttcagc ggcggccagc aagcgcttgt
     6781 cttcatagtc cttgacggcc cactgccatt ccagcgcgcc ttcgtagcgc agaccgatgg
     6841 cgtacagcgt cagtgcgcgt acgatgccgg ggtccttggc ggtcgcctcg atttcgtcct
     6901 cgtcgggccg gtaggtttgc ggcaccgtgc tgatcgaggt gccgagttcc tccgcggcga
     6961 gctggccgta gaagttgaac tggttggaaa gcggcgccag cagcgcgttg gcttccgact
     7021 ggcggcccag cgccgacaac gcgcgcgctt tccagtagcg ccattgcgca tatttgtact
     7081 ccgtcggcga catcgcctcg atggcggcga acacctcgtt ccagtcgcct gcgcgcagcg
     7141 cggagcgggc acgccaggtg agctggcgat cgttcatgcc gttgcccgcg ttcctgaacc
     7201 aggtaagcgc gtccggatga aggctgcgcg cggcgaccga ggcgatttgc gcccacgcgt
     7261 actcacgctc ggtgtccggc aaatccttgg cgattctgtg cagccggtcc gcggcggttt
     7321 gcggccagcc ctctgcggtc ttgtacagcg cgtagatcac cagttcgcgc tgagcgcggg
     7381 tcttcaacgg cagcggggcg cggtccagaa aatgctgcgg ccggcgcgct atcgaatcga
     7441 gggtcttcgc atccggtcgt tgcccggccg gcagataacc gagcaacatc ctggcgccgc
     7501 tcaagttgcc gatttccagc gcaagcctga tccgggtcca gctgtcctcg gctgtgagca
     7561 tgtctttctt gaacatcaat tcgaacaagg gcaggcagct ttccgggagc acctgcgcgg
     7621 tgaaccacag cggccgtgcc tgcgtcagta tcccggactc gcccagcgca aggcgcgcct
     7681 gataggcaaa gcagaccagc tcgaggtctt cggtggtgag ctgcgggtat tcgcgcagga
     7741 aacccggcca atcgcgatcc ttcgccagtt gccgcagcca gtccgcgcgc aagcggtcgg
     7801 cggccaggct gttgttgtga cgggccagga agttctctat ctcgccggca gacaggtcgg
     7861 cgaggcgcga acgcaattgc cagtactgca catacagggc tagcggatag ttgtcgtcga
     7921 gctgcttggc aaggcggtcc agccgcacga tgtcgccgcg ctggaacgct tcgcgcgcct
     7981 cgaggaaaac ttcgtcgtcc ggcgccgcca atgcagcgca ggaggcgaac agcagtacgg
     8041 caaggctttt tttcaaggtg tcagtctgtg agcagcagga tgagtcggtc aacgcgtaat
     8101 tataaaaggg cggcctgaga aatagagcat gatttaggcc agaaacagcg aataggccgg
     8161 gttcctggtc tcgtccgagt gcgcgtagcc gagttgcgag agaaacctgc ggaacgccgc
     8221 ctgatcgcgc ggcggcacct gcattcccac cagcacgcgc ccgtaatcgg cgccgtgatt
     8281 gcggtagtgg aacaggctga tgttccagtc gtgactcatg ctgtcgagga aattcatcag
     8341 cgctcccggg cgctccggga actcgaaacg gtacagcctc tcgttctcga ccgagggcgc
     8401 acggccgccc accaggtggc gcacatgcag cttggccatt tcgttgtcgc tgaaatcgag
     8461 cgtcggcagc cctttgcggc gcagccgcgc aatgagcttt cgggtctcat cgcggttgcg
     8521 cacctgcacg ccgacgaaca catgggcttc cacggggtcg gcgtagcgat agttgaattc
     8581 ggtgacattg cgcgccccta gcagggagca gaactgcttg aagctgccgg gacgctcggg
     8641 gatcgtgacc gccaggatgg cttcgcgctt ctcgcccagt tcggcgcgct cggcgacgaa
     8701 gcgcagccgg tcgaagttca tgttggctcc gcaggcgacc gcgacgaacg tccggccttt
     8761 gtgcttgtgg cgctcggcgt aagccttggc gccggcgatc gcgagcgcgc ccgccggttc
     8821 caggatcgag cgggtgtcct cgaatacgtc cttgatcgcg gcgcagacgg catcgttgtc
     8881 caccaggatc atctcgtcca ccagctcccg gcaaagacgg aaggtttcgg agccgacctg
     8941 cttgaccgcc acgccgtcgg caaaaagtcc gacttgcgcg agcctcaccc gatggccggc
     9001 agcaagggag cgggtcatcg cgtcggcatc gaccggttcc acgccgatga tccggatctc
     9061 cggacgcagg cgcttgacgt aagccgcgat tcctgcgatc agcccgccgc cgcccacggc
     9121 gacgaaaatg gcgtcgatcg gcccaccgtg ctggcgcagg atctccatgc cgatggtacc
     9181 ctgcccggcg atcacatccg ggtcgtcgta gggatgcacg aaagtgagct tctcgcgcct
     9241 ggcgatcttc agcgagtgcg cgtaggcctc gtcgtaggag tcgccgaaca gcaccacccg
     9301 cgcaccgcgc gccgccaccg ccgcaatctt gatctgcggc gtggtggcag gcatgacgat
     9361 ggtggcgcgg cagcgcagat gatttgccgc cagcgccacg ccttgcgcgt gattgcccgc
     9421 cgaggcggcg atcacgccgc gccggcgctg ggcagcgctc aggttcgcca tcttgttgta
     9481 ggcgccgcgc agcttgaagg agaacaccgg ctgcatatcc tcgcgcttga gcagcagggt
     9541 gttacccagc cggccggaaa gcgccggcgc cagctccaga ggcgtttcga ctgcaacgtc
     9601 ataaacgcgc gcagtcagga tcctttccag gtaatcgatc ttcatcgctt ctcccaggtt
     9661 tccttcgagc gtaacagccg ggaacgaacg ggcgcaaccg gggcgcttat ttgtgccgat
     9721 caggcgttga cgctatgctt gcaggcagtt ccagacgcag caggaccatg actcaggacg
     9781 aactcaagaa ggcggtcgcg aaagcggcga tcgcccacgt gccggaagac tgcatcgtcg
     9841 gcgtcggcac cggctcgacc gcgaatttct tcatcgacga actggccagg atcaagggac
     9901 ggctggaagg cgcagtggcc agctcggagg cgagcgcagc ccggctgaaa gtcgcgggaa
     9961 tccgcgtgtt cgacctgaac tccgtggacg aactgccggt ctacgtggac ggcgccgacg
    10021 agatcacgcg gcatctgcac atgatcaaag gcggaggcgg tgcgctcacc cgggagaaga
    10081 tcgtggcggc ggtggcgagc aagttcgtct gcattgccga cgaagccaag ctgaaggacg
    10141 ttctgggcgc gtttccgctg cctgtggaag tgattcccat ggcgcgttcc tacgtcgccc
    10201 gtgagctggt caggctgggc gggcacccgg aattgcgcca gggctttacc acggacaacg
    10261 gcaatatcat cctggacgtg cgcgggctgg caatcgtcaa tccggtggcg ctggagggcg
    10321 aaatcaacca gatcgtcggg gtggtggcga acggcctgtt tgcccgccgc ggcgccgatg
    10381 tgctgctgct cggcacccgg gacggggtga agacgttgac ccggtgaaac ttttttgtaa
    10441 cgcaatcgac gtacactggg acatttgccg gtgcgcggta cggaaggggg ttcgaagtga
    10501 tgaccaattc tgagcacaca tccaagcagt tcgatgccga actggaggcg gtgcgcgccc
    10561 gcgtgttgca catgggcggg ctcgtggaag agcagatcaa gctggcgctg gacgccctga
    10621 tttcgggcga cctcgagctt tgcaccaagg tcgagaacaa cgatcacatg gtcaacggcc
    10681 tcgaagtcgg catcgacgag gactgcagca ccatcatcgc ccgccgccac ccggccgccg
    10741 gcgacctgcg catgctgatg acggtggtga agaccattac cgacctggag cgcatcggcg
    10801 acgaagcggc gaagatcgcg cgcatggcga aattgatcta ttccgccgac cggctgcagc
    10861 agccgcgcac c
//
LOCUS       DASQOP010000166         3023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2885542, whole genome shotgun sequence.
ACCESSION   DASQOP010000166 DASQOP010000000
VERSION     DASQOP010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2885542"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..131)
                     /locus_tag="VE030_06695"
     CDS             complement(<1..131)
                     /locus_tag="VE030_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZF23137.1"
                     /translation="MEAFLHQVLSGLATGGIYASLALALVMIYQSTSHINFAQGEMA"
     gene            complement(366..1076)
                     /locus_tag="VE030_06700"
     CDS             complement(366..1076)
                     /locus_tag="VE030_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZF23138.1"
                     /translation="MALLEVSGLHAGYSAADVLRGVAFAVEQDGITTVLGANGAGKTT
                     LLRAVSGMVRRQGSVRFDGRPIETLATEEIARLGIGHVPDGRGTFFDFTTEENLRIGA
                     YTRKDRHAVARDFERVYNYFPRLKERRQQQAGTLSGGEQQMLAIARALMLSPRLLLLD
                     EPSFGLAPLVVQDIFRIMRSINAEEGVSVLLVEQNAALALDLADHAYLLETGEVVMSG
                     SADTIRSNETVRRAYLGY"
     gene            complement(1077..1877)
                     /locus_tag="VE030_06705"
     CDS             complement(1077..1877)
                     /locus_tag="VE030_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZF23139.1"
                     /translation="MDNAAPGGDPRGQPLLRVSGLSVQFGGIVALEDVSFDVMQGQIA
                     GVIGPNGAGKTTLFNCLSRLYPFQGGSIYFRAQSLLEIPRHRIADCGIGRTFQNLALF
                     RTMSVLDNIMVGAHCRSRGGFLANALRMPVVAREESRVETKARGLIELLDLQAVAQKR
                     VADLPFGTQKRVELGRALAAEPRLLLLDEPAGGLNHEEVGSLSALIRRIRDQLGVTIL
                     LVEHHMSLVMQLSDKVVALNFGRKIADGTPAEVQRNPDVIRAYLGKDA"
     gene            2376..>3023
                     /locus_tag="VE030_06710"
     CDS             2376..>3023
                     /locus_tag="VE030_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017330058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZF23140.1"
                     /translation="MEPSHYRFWPENVPREIALPRASVYHNLASAAAREPDKTALIYY
                     GSLLTYRTFKSEADALAGFLQRRCGVDKGDRVLLFMQNSPQSMIAFYAILRADAMVVP
                     VNPMNLTDELRHYVADSDARVAITGQELFPQLRPLIGAGLDHVLVAAYSDYLAQPAGL
                     KVPEAAAAPRQTIREPGVTLWGDALAANIAPGPHRAGPDDLCVMPYTSGTTGKPKG"
BASE COUNT          585 a          982 c          921 g          535 t
ORIGIN      
        1 atcgccattt cgccctgcgc gaaattgatg tggctggtgg actggtagat catcaccagc
       61 gcaagcgcca ggctggcgta aatgccgccg gtggccagtc ccgacaacac ctgatgcaga
      121 aacgcttcca ttgccgctcc ccgactagtg tcctgttccg ctaattcgcg gctgaaaacc
      181 cggcgagaat cggcgccatg cggtgttacg agtcctcgcc gtggtgcccg ccactgtcaa
      241 ggactcgcgc cttgcctgac accgatttcg cgggttttct gatcgatgac ggatcgttgt
      301 catatgtata ggaggtctaa cttccaaagt tcgcaagttg tcccacgaat aatcggaaca
      361 ggacactagt aacccagata ggcgcgccgc acggtttcgt tggagcggat ggtatcggcg
      421 ctgcccgaca tcaccacctc cccggtttcg agcagatagg cgtgatcggc gagatccagc
      481 gccagtgccg cgttctgctc caccagaaga acgctgaccc cctcctctgc gttgatactg
      541 cgcatgatgc ggaaaatatc ctgaaccacc agcggcgcca agccgaagga aggctcgtcg
      601 agcagcagca gcctcggaga gagcatgagc gcccgggcaa tcgccagcat ctgctgttcg
      661 ccgcccgaga gtgtgcctgc ctgctgctgc cgccgctcct tgagccgcgg gaaatagttg
      721 tagacgcgct cgaagtcgcg tgccaccgcg tgccggtcct tgcgggtata ggcgccgatg
      781 cgcaggtttt cctcggtggt gaaatcgaaa aaagttccgc ggccatcggg cacatgcccg
      841 attcccagcc gcgcaatctc ctcggtggcc agggtctcga tcggccggcc gtcaaagcgc
      901 accgagccct gcctcctcac catgccgctc acggcgcgca gtagcgtggt cttgccggca
      961 ccattggcgc cgaggacagt agtgatgccg tcctgctcga cggcgaaagc cacaccgcgc
     1021 agaacgtcgg cggcgctgta accggcgtgc aagcccgaga cctcgagtaa cgccattcag
     1081 gcgtcctttc ccaggtaggc gcgaatcacg tccgggttgc gctgcacctc cgccggggtg
     1141 ccgtcggcga ttttcctgcc gaaattgagc gctaccacct tgtcggagag ctgcatcacc
     1201 aggctcatgt gatgttccac cagcaggatg gtcaccccca actggtcgcg gatgcggcgg
     1261 ataagcgcgg aaagcgatcc cacttcctcg tggttcagcc cgcccgccgg ttcatcgaga
     1321 agcagcaacc ggggctcggc tgccagggcg cgtccgagtt ccacgcgttt ttgcgtaccg
     1381 aacggcaggt ccgccactcg tttctgcgca accgcctgca gatccagcag ctcgatcagg
     1441 ccccgtgctt tcgtctcgac ccgcgattcc tcgcgcgcca ccaccggcat gcgtagggcg
     1501 ttggcgagga aaccgccgcg gctgcggcag tgggcgccga ccatgatgtt gtccagaaca
     1561 ctcatggtcc ggaacagcgc aagattctgg aaggtgcggc cgatgccgca gtcggcaatg
     1621 cggtggcgcg ggatttcgag cagcgactgc gcgcggaagt aaatgctgcc gccctgaaac
     1681 ggatagaggc gcgacaggca gttgaacagg gtggtcttgc cggcgccgtt gggaccgatc
     1741 acgccggcga tctggccctg catcacatcg aaggacacgt cttccagcgc aacgatgccc
     1801 ccgaactgaa cgctcagtcc ggaaacccgc aacaacggtt ggccacgagg gtcgcccccc
     1861 ggcgcggcat tgtccatttt ttcttcgccc acggtgctcg ccaactccaa gccctccccg
     1921 atgtgggatg cgccataccg aaacgcgcac tggatactgg cacaaccgcc gtgaccggta
     1981 aagttatcgc cggaccggtc ttcgctgcat tgcaatgcgg ttcatcttgc ccgaaccttg
     2041 aaccacggag gacacagagg acacagagga cacggaggaa aaccagaatc aaggaaaaac
     2101 atgtgggagt taagagcaag cccggtgccg aagtgacctc ccagccttaa ctttcgaacg
     2161 ctctttgccg gcccttgcct ttcctccgcg tcctctgtgt cctccgtggt tcacgaactt
     2221 gacctatttc ggaggagcag cgttgaccgc ggaggcgggc atgtccaaca tagtcgggca
     2281 cggcgccgca aggaccggtc cgcgagaaac tgccaaggca ggtccgacga caaaaagagg
     2341 cctctgcaaa gatacggcac gaggaggaac aagaggtgga accgagtcat taccgtttct
     2401 ggccggagaa cgtgccgcga gaaatcgcgc tgcctcgggc gagcgtctac cacaatctgg
     2461 cgagcgcggc cgcacgggaa cccgacaaga ctgcgctgat ctactacggt tcgctgctca
     2521 cctaccggac tttcaagagc gaggcagacg cgctcgccgg attcctgcaa cggcgttgcg
     2581 gcgtggacaa gggcgatcgc gtgctgctgt tcatgcagaa cagtccgcag tccatgattg
     2641 cgttctacgc gatcctgcgc gcggatgcga tggtcgtgcc ggtgaacccg atgaacctca
     2701 ccgatgaact gcgccactac gttgccgaca gcgatgccag ggtggcgatc accgggcagg
     2761 agctctttcc ccagctccgc ccgctgatcg gcgcgggcct cgatcacgtc ctggtggccg
     2821 cctattccga ttatctggcc cagcctgccg gcctgaaggt gccggaagcg gcggccgcgc
     2881 cgcgacagac aatccgtgaa ccgggggtca cactgtgggg cgacgccctc gctgccaata
     2941 ttgcgcccgg gccgcaccgt gccggcccgg acgacttgtg cgtcatgccc tatacttcgg
     3001 gcaccaccgg caagcccaag ggc
//
LOCUS       DASQOP010000167         7968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2283599, whole genome shotgun sequence.
ACCESSION   DASQOP010000167 DASQOP010000000
VERSION     DASQOP010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7968
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2283599"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(402..1619)
                     /locus_tag="VE030_06715"
     CDS             complement(402..1619)
                     /locus_tag="VE030_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HZF23141.1"
                     /translation="MKITAIETIGLEEFPNILWVHIHTDEGIVGLGETFYAVAPVAEH
                     IHQTCAPYLLGKNPLEIDRHSRQFLNTYLGFNSVGVEMRAASAVDVALWDLFGQFTGQ
                     PIYQLLGGASRDKVRVYNTCAGYQYVRAKPTQGTENFGLPAKADKKNRPYEDLMAFKT
                     DAGELAKSLLEMNITGMKIWPFDEAAEASNGSYISNADLKKAMRPFEQIRKAVGDKMD
                     IHVEFHSMWNLPTAIRIAEALEQFDPFWYEDPIKMNNIDAVADYAHRTNVWVTASETL
                     GTRWGFRELFEKQAVSVCMLDVGWTGGLSEAKKIATMAEAYALPVAPHDCTGPILLTA
                     SVHLSLNCPNTLVQEMVRAFYYDWYDNLVTQLPPVKDGYITAPKGPGLGTKLQASVLK
                     RKDVVRRISKLGK"
     gene            1895..2371
                     /locus_tag="VE030_06720"
     CDS             1895..2371
                     /locus_tag="VE030_06720"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZF23142.1"
                     /translation="MFREDLSRNFGFILNDVARLMRTTFDRRVKSLGLTRSQWWVLNH
                     LFRNDGVTQSELADILEVKKATLGRLLDRMEQKGWIRREGHAGDRRAKRVFLTDEVEP
                     AIKTMRAAAAEVRRDALSGLSSAQQDQFVDALLAIKGNLSKQDNGANGDGAKKRKR"
     gene            2373..3500
                     /locus_tag="VE030_06725"
     CDS             2373..3500
                     /locus_tag="VE030_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HZF23143.1"
                     /translation="MSEAASRAVIVVHRAVRILLLVVVPLVAVVAGLYVYATGGREVE
                     TDNAYVKANIVPISASVNGRVIEVLARDNQPVEAGALLFRLDPEPYQIAVDGARAQLD
                     VVRTDVQSLRAEYRATLQEAAEVRSRIDFLEKQLARQEYLKEKGMSRGDTYDEARHNV
                     DAARRRLDSIREKSNRVIAELSGNPDMPVERLPRYAEARAAYDAAMLDLSRALIKAPF
                     AGVVSNMKLQVGEFVEKGAPMFSLIESGPLWIEANYKETQLTHMTVGQSAKVVADAYP
                     DHEWPASVEAIAPATGAEFAVLPPQNATGNWVKVVQRVPVRIKVEQPPGKPQLRAGMT
                     VTVTVDTGRPRGLPRTLQKLVDDGWLPRFLVPSSAIAGTRR"
     gene            3502..5034
                     /locus_tag="VE030_06730"
     CDS             3502..5034
                     /locus_tag="VE030_06730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HZF23144.1"
                     /translation="MTGSSRHPALITASVGLASFLYSIDWTIAAVALPHMRGTFSATQ
                     DQIAWVITSYIVASALSIPAAGWLSLRFGRKRVFMWAVAVFLLASVACGAANSLAVEV
                     SARIVQGLGGAFLIPLSHAIILDTYPPEEQGKAMALWGMAAVMGSFVGPTLGGYVTEY
                     LSWRYIFYINVPFGLLALAGAAAFLPETKRDPGRRLDWFGFLSLSLGVGSLQLMLDRG
                     GQLDWFESWEIITEACLAVLGLYMFNVHCLTAKQPFLDPRLLAQRSFFLGLVFAFIYG
                     SITTPPMVLMPSFLDQVRGYPIDTIGLLQAPRGIGLFCAMIIGGRTTGRIDPRKLIAF
                     GLLCLAYSSWEMSTWTAEVGVWPLVWTNFMQGIGGGIILVPIQVIAFPSLEPHRRTEA
                     TAVYNLVRSIGASIGVSGALAMYVRASSVMHAQLVEHATPFNRALQAQGHEGWGMATT
                     QALARLEREISLQSAVIGYTGDFWLFALVALAALPLLLFIGRQTLPSGAGDRAGGLTI
                     GE"
     gene            5092..5961
                     /locus_tag="VE030_06735"
     CDS             5092..5961
                     /locus_tag="VE030_06735"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HZF23145.1"
                     /translation="MNTVDRPIRPRRSLLFVPGLRPDRFAKALDSGADIVCVDMEDAV
                     ARPRKDEGRALTLPLFEKNTHPHVEQMVRINGLSTPDGLKDLTAIIESEAPPPAIMIP
                     KIRSAEEVQLIETLLSAGPARNIRFCVIIETNQGLERALEITRASSRIDSVILGAVDM
                     SAELRCEKSWEPLLYTRSRLVHAAASAGIDLLDVPFLNLDDPEGLRQEAQACARLGFT
                     GKASIHPNQIPIINAAFTPDEKTIAKARKVCAAFEQDKTGLVVVDGELIELPVVRSMY
                     RVLAIASRVTRAA"
     gene            complement(6023..6436)
                     /locus_tag="VE030_06740"
     CDS             complement(6023..6436)
                     /locus_tag="VE030_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23146.1"
                     /translation="MSRIKKSRFLAATLVAMAVVAPAQAGDFTTLKENIFVCISPQAY
                     DEAMVRVRELGGRNIEPLKKELGENKLCMFVDPEMADNIMAPFAVVLQRDGTKVQVQF
                     IVTFRKKVEFLHRLINRYVLVGWTDESNLETRRVL"
     gene            complement(6433..6879)
                     /locus_tag="VE030_06745"
     CDS             complement(6433..6879)
                     /locus_tag="VE030_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZF23147.1"
                     /translation="MRIASIVFVSVAALGVSHAGLLAAMEQGKLEPKIAQTQTAQAEN
                     GPVKVPSSNPLSGDPEAIDSGKHLYFSWCVQCHGPKADGDSPRFGKYAGNLTKFWRGY
                     PEFVNTVKNGRPGKQMPPWKEFMDEDTIAKLGAYLETLAVEGANWK"
     gene            complement(7054..>7968)
                     /locus_tag="VE030_06750"
     CDS             complement(7054..>7968)
                     /locus_tag="VE030_06750"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZF23148.1"
                     /translation="DPNADGWRSGDALYTSSTVALDLNTGKLNWYFQEAHADPYDYDA
                     APGEYVILGDKVVHPGKNGFNAVHQTKGGKVLAVYPDMKSFNWTTGYNIAKGEWENML
                     WPQPGEKTLVCPAIDGGHSWQAGSYSPKTKLFYRVAQEWCMYLTAQPKSGGTTISAGA
                     ETRTLQPFAQAFMSAEWIGTDPPGDKSHGRLTARDPVTGKLAWEKRYDLIPHSTLMST
                     GSDLLFVGTSDGFVEALDAKNGNVLWRYNVGSGVHGGIISYAVDGKQYIAVASGHGTY
                     VGGAIKALNKEKLGTMNESMALVVFALP"
BASE COUNT         1393 a         2505 c         2474 g         1596 t
ORIGIN      
        1 ttcttgaacg cgaacttgtg gccgacttca acgcgggcct tgagcgtcac ggactggccc
       61 ttcaccgaca gcgtcgtgcc cgaagcgatg tccgacttgg cgatcgccac gttgtcgccc
      121 ggcgccaata ccatcactcg cgcgtcggtg ctcgttgcaa tttcgttcat atctaaacct
      181 caataaggac taggcgctca ccgcagaggg cgcggaggtc gcagaggaaa cgcgagagca
      241 agaatatgct cgaggtgaaa cccttcattg actgttgatc catcgtagtt aaagttgact
      301 catgcgttcg cgaactgttt cccgcttttt acccacaatt tggttttcct cgtttttgtt
      361 tttcctccgt gtcctctgcg tcctccgcgg tgagtgttgt cctatttccc caatttcgaa
      421 atccgccgta cgacatcctt gcgtttcagc accgatgcct gcagcttggt gcccagccca
      481 ggccccttgg gcgcggtgat gtagccgtcc ttcaccggcg gcaactgggt gaccagattg
      541 tcgtaccagt catagtagaa cgcccgcacc atctcctgca ccaatgtatt gggacagttc
      601 agcgacaggt gcaccgaagc ggtcagcagg atcggcccgg tgcagtcgtg cggagcgacc
      661 ggcaacgcgt aggcttccgc catggttgcg attttcttgg cctcggacaa tcctccggtc
      721 cagccgacgt ccagcatgca caccgagacg gcttgtttct cgaacagctc gcggaagccc
      781 cagcgcgttc cgagggtttc cgacgcggtg acccagacat tggtgcgatg ggcgtagtcc
      841 gcaaccgcgt cgatgttgtt cattttgatc ggatcttcgt accagaacgg atcgaactgc
      901 tccaatgctt ccgcgatgcg aatcgcggtg gggagattcc acatcgaatg gaattcgacg
      961 tggatgtcca ttttgtcgcc tacggccttg cggatctgct cgaacggccg catggccttc
     1021 ttcaggtcgg cattcgagat gtagctgccg ttggaggctt ccgcggcctc gtcgaacggc
     1081 cagatcttca tgccggtgat attcatctcc agcagtgact tcgccagttc gccggcatcg
     1141 gtcttgaacg ccatcaagtc ctcgtaaggc cggttcttct tgtcggcttt cgccggcagg
     1201 ccgaaattct cggtgccctg ggtcggcttg gcgcgcacgt actggtagcc ggcgcaggtg
     1261 ttgtagacgc gcaccttgtc gcgcgaagcg ccgccgagca actggtagat cggctggccg
     1321 gtgaactggc cgaacaggtc ccacaaggcg acgtcgaccg cagaagccgc gcgcatttcc
     1381 acgccgacgc tgttgaatcc gagataggta ttgaggaact ggcggctgtg cctgtcgatc
     1441 tccagcgggt tcttccccag caggtatggg gcgcaggtct gatggatgtg ctcggccacc
     1501 ggggcgaccg catagaaggt ctcgcccagt ccgacgatgc cttcgtcggt gtggatgtgc
     1561 acccagagaa tattggggaa ctcctcgagg ccgatggtct cgatagcggt tattttcatc
     1621 ttgtggttct tcctccctga ggtgacgccg attctagcag ggcgttaaca aaaagaactc
     1681 accgccaagg acgccaggga cgccaaggaa ggtcaaaggc aagatcgggc aaaaacagca
     1741 ccccgcgagt ccgggccagc agacattcct tgttactcct tggttttcct tcgcgtcctt
     1801 ggcgtccttg gcgtccttgg cggtgaatgg tcttgcggat gataacctgg catatagtaa
     1861 gctaggcgac caatttgggg tagaatccgc agccatgttc cgcgaagacc tgtcgcgcaa
     1921 tttcggcttc atcctgaacg acgtcgcccg tctgatgcgc accaccttcg accggcgcgt
     1981 aaagtcgctc ggcctgacac gctcgcagtg gtgggtgctc aaccatctgt tccgcaacga
     2041 cggcgtgacc cagtccgagc tggccgacat cctcgaagtg aaaaaggcga cgctgggacg
     2101 cctgcttgac cgcatggagc agaaaggctg gattcgccgc gaaggccatg ccggcgaccg
     2161 gcgtgccaag cgcgtgttcc tgaccgacga agtggagccc gccatcaaga ccatgcgcgc
     2221 ggccgcggcg gaagtccggc gcgacgcgct gtccggcctg tcttccgccc agcaggacca
     2281 gttcgtggac gcgttgctcg ccatcaaggg taatttgtcc aaacaggaca acggcgccaa
     2341 cggcgacggc gccaaaaagc gcaagcgctg acatgtccga ggcagccagc agggcggtga
     2401 tcgtcgtgca tcgcgcagtg cgcattctgc tgctcgtggt cgtgccgctg gtcgcggtcg
     2461 tggccggcct ttatgtctat gcgaccggcg ggcgggaggt cgagaccgac aatgcctacg
     2521 tcaaggccaa catcgttccg atcagcgcat cggtgaacgg gcgtgtcatc gaggtgctgg
     2581 cacgcgacaa ccagccggtc gaggcgggcg cgttgctatt ccgcctcgac cccgagccct
     2641 accagatcgc cgtggacggt gcgcgcgccc agttggatgt ggtgcgaacc gatgtgcagt
     2701 cgctgcgcgc cgaatatcga gcgacgctgc aggaagccgc cgaagtgcgc agccgcatcg
     2761 actttctcga gaagcagctc gcgcgccagg aatacctcaa ggaaaaaggc atgtcgcgcg
     2821 gcgacaccta cgacgaggcg cgccacaacg tcgacgccgc caggcgccgg ctggacagca
     2881 ttcgtgaaaa gagcaaccgc gtgatagcgg aactgtcggg caatcccgat atgccggtcg
     2941 aacgccttcc gcgttacgct gaggcgcgcg cggcctacga cgcggccatg ctcgacctgt
     3001 cgcgtgcgct gatcaaggcg ccgttcgccg gcgtggtgag caacatgaaa ctgcaggtcg
     3061 gcgaattcgt ggagaagggc gcgccgatgt tcagcctcat cgaaagcggc ccgttgtgga
     3121 tcgaggccaa ttacaaggaa acgcagctta cccacatgac ggtcggtcaa tccgccaagg
     3181 tggtggcgga tgcgtatccg gatcatgaat ggccggccag cgtcgaggcc atcgcgccgg
     3241 ccaccggcgc cgaattcgcg gtgctgccgc cgcagaacgc aaccggaaac tgggtgaagg
     3301 tcgtgcagcg cgtgccggtt cgcatcaagg tcgagcagcc gcccggaaag ccgcagcttc
     3361 gtgccggcat gacggtgacc gtgacggtgg ataccgggcg gccgcgcgga ctgccgcgca
     3421 cgctgcagaa actcgtagac gacggctggt tgccgcgctt cctcgtgccg tcttcggcca
     3481 tcgcaggcac ccgccggtaa cgtgaccggt tcctccagac atcctgccct gatcaccgcc
     3541 tcggtcgggc tggcgagttt cctgtattcg atcgactgga ccatcgccgc cgtggcgttg
     3601 ccgcacatgc ggggcacgtt ctcggcgacg caggatcaga ttgcctgggt catcacttcc
     3661 tacatcgttg cctccgcgct ctccattccc gctgcgggat ggctgagcct gcgcttcggc
     3721 cgcaagcgcg tattcatgtg ggcggtcgct gtttttctgc ttgcctcggt cgcatgcggc
     3781 gcggcgaatt cgctggcggt tgaagtctcc gcgcgcatcg tgcaggggct gggcggcgcg
     3841 ttcctgattc cgctgtcgca cgccatcatc ctcgatacct atccgccgga agagcagggc
     3901 aaggccatgg cgctatgggg catggccgcg gtgatgggtt ccttcgtcgg gcccacgctc
     3961 ggcggttacg tcaccgagta cctgagctgg cgttatattt tctatatcaa tgttccgttc
     4021 ggcctgctgg cgctggccgg cgctgcggcg ttcctgcccg agacgaagcg cgatcccggg
     4081 cgccggctcg actggttcgg attcctcagc ctgtcgctgg gtgtcggttc gctgcaattg
     4141 atgctggacc gcggcgggca actcgactgg ttcgaatcct gggagatcat cactgaagcc
     4201 tgtctcgcgg tcctgggcct ttacatgttc aacgtgcact gcctgacggc gaagcaacct
     4261 ttcctcgatc cgcgcctgct ggcgcaacgc agtttctttc tcgggctggt attcgccttc
     4321 atctacggtt ccattaccac gccgccgatg gtgctgatgc catcgttcct cgatcaggtg
     4381 cggggctacc ccatcgacac gatcggcctg ctgcaggcgc cgcgcggcat cggcctgttc
     4441 tgcgcgatga tcatcggcgg acgcaccacg gggcgcatcg atccgcgcaa gctgatcgcg
     4501 ttcggcctgc tgtgcctcgc ctactcgagc tgggagatgt cgacctggac cgcggaggtc
     4561 ggcgtgtggc cgctggtgtg gacgaatttc atgcagggca tcggcggcgg catcatcctg
     4621 gtgccgatcc aggtcatcgc atttccgtcc ctggagccgc atcgccgcac cgaggccacc
     4681 gcggtctaca acctggtgcg cagcatcggc gcgagcatcg gcgtgtcggg cgcgttggca
     4741 atgtatgtgc gcgccagcag tgtgatgcat gcgcaactcg tcgagcacgc cacgcctttc
     4801 aaccgcgcat tgcaggcaca gggacacgaa ggatggggca tggccacgac gcaggcgctg
     4861 gccaggctcg aacgcgaaat cagcctgcag tccgctgtga tcggctacac gggtgatttc
     4921 tggctgtttg cgctggtcgc gctcgcagcg cttccgttgc tgttgttcat cgggcgacag
     4981 acgcttccca gcggcgcggg cgatcgcgcc ggggggctca cgatcggcga atagactggc
     5041 gcgccggctc cccggcagct agcattcggg tttttccctc gcgaccccgg catgaatacc
     5101 gttgatcgtc cgatacgtcc gcgccgcagc ctgcttttcg tgccggggct gcggccggat
     5161 cgtttcgcca aagcgctcga tagcggagcc gacatcgtct gcgtggacat ggaggatgcg
     5221 gtggcgcgcc cccgcaagga cgaagggcgc gcgctgactc tgccgctgtt cgaaaaaaac
     5281 acccaccccc acgtcgagca gatggtgcgt atcaacgggc tctcgacgcc ggacgggctc
     5341 aaggacctga ccgcgatcat cgaatccgaa gccccacccc cggcgatcat gatcccgaag
     5401 atcaggtcgg ccgaggaagt gcagctgatc gagaccctgc tctccgccgg ccccgcgcgc
     5461 aacatccgct tctgcgtgat catcgagacc aatcagggtc tggagcgcgc cctggagatc
     5521 acgcgggcat cctcgcgcat cgactcggtg attctcggcg cggtggacat gtccgccgaa
     5581 ctgcgttgcg agaaatcctg ggagccgctg ctctataccc gctcgcggct ggtgcatgcg
     5641 gcggcctccg ccggcatcga tctcctggat gtgccgttcc tgaacctcga cgatccggaa
     5701 gggttgcggc aggaggcgca ggcctgcgcc cgtctgggtt tcaccggcaa ggcctcgatt
     5761 catcccaacc agatcccgat cattaacgcg gctttcacgc cggacgagaa gactatcgcg
     5821 aaggcccgca aggtctgcgc cgctttcgaa caggacaaga ccggcctggt ggtggtggac
     5881 ggagaactga tcgagttgcc ggtggtgcgt tcgatgtacc gggtactggc gatcgccagt
     5941 cgagttacgc gagcggcgtg aggcgggaag acaattcgtt accgggactc gcgacctcta
     6001 cacgtcacag ccgcgagcag cgctacagga cgcgcctcgt ctccaggttc gactcgtccg
     6061 tccagccgac gagtacgtag cggttgatca gccggtgcag aaactcgact ttcttgcgaa
     6121 acgtgacgat gaactggact tgcaccttgg tgccgtctct ctgcagcacg acggcgaagg
     6181 gcgccatgat gttgtctgcc atctcgggat cgacgaacat gcatagcttg ttctcgccca
     6241 gttccttctt caacggctcg atgttgcggc cgccgagttc gcgcacgcgc accatggcct
     6301 cgtcgtaggc ctgaggcgag atgcacacga aaatgttctc cttcagcgtg gtgaaatcgc
     6361 ccgcctgcgc cggtgcaacc actgccatgg ctaccagcgt tgccgccaga aagcggctct
     6421 tcttgattcg gctcatttcc agttcgctcc ttccactgcc agcgtttcca ggtaggcgcc
     6481 caacttggcg atggtgtcct catccataaa ttccttccag ggcggcatct gctttcccgg
     6541 gcggccgttc ttgacggtgt ttacaaactc cgggtagccg cgccagaact tcgtcaggtt
     6601 gccggcatac ttaccgaaac gcggcgaatc accgtcggcc ttcgggccgt ggcactggac
     6661 gcaccaactg aagtacaggt gcttgcccga atcgatggct tcgggatctc ccgacaaggg
     6721 attggaagag gggaccttga cggggccgtt ctctgcctgg gccgtttggg tctgggcgat
     6781 cttgggctcg agttttcctt gttccattgc cgcgagcagc cccgcgtggg acacgccgag
     6841 tgcggcgacg gacacgaata cgatggacgc aatgcgcatt ttggatttca taccccatca
     6901 aaaattctcg ccttcgcgcc atccggacgg cggctgcgaa gacgagaagg acactaccac
     6961 acaatttccg ccgggcttcg aggcgccggc ggagcaaaaa aacgccgggg cggcaggagg
     7021 ttaccctccg tccgtccccg gcaggggaga gggctatgga agtgcgaata ccaccaatgc
     7081 catgctctca ttcatagttc cgagcttctc cttgttcagg gccttgatcg ccccgcccac
     7141 gtacgtgccg tgtccggatg ccactgcaat gtactgcttg ccgtcgaccg cgtaggagat
     7201 gatgccgccg tgcacacctg agccgacgtt ataccgccac agaacgttgc cgttcttggc
     7261 gtccagcgct tcgacgaagc catcagaggt gccgacgaac agcaggtcgc tccccgtgga
     7321 catcaacgtc gaatgcggga tcaggtcata gcgcttctcc caggcgagtt tcccggtaac
     7381 cgggtcacgc gcggtcaggc gaccatgact cttgtctccc ggcgggtcgg taccgatcca
     7441 ttcggcactc atgaacgcct gggcgaaagg ctgcagggtc cgggtctcgg cgcccgccga
     7501 gatggtggtc ccgccgctct tcggttgcgc ggtcaagtac atgcaccatt cctgcgcgac
     7561 gcgatagaac agcttggtct tcgggctgta gctcccagcc tgccagctat gcccaccgtc
     7621 gatcgccggg caaaccaggg tcttctcgcc gggctgtggc cacagcatgt tttcccattc
     7681 acccttggcg atgttgtagc ccgtcgtcca gttgaaactc ttcatgtctg gataaacggc
     7741 caggaccttg ccgcccttgg tctggtgcac agcgttgaag ccgttcttgc ccggatgcac
     7801 caccttgtct ccgagaatga catactcgcc cggtgcggcg tcgtagtcgt acggatcggc
     7861 gtgcgcctcc tggaagtacc agttgagctt tccggtgttc agatccagcg cgaccgtgga
     7921 cgacgtatac agcgcgtcac ccgagcgcca gccatcggca ttcggatc
//
LOCUS       DASQOP010000168        24497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_69697, whole genome shotgun sequence.
ACCESSION   DASQOP010000168 DASQOP010000000
VERSION     DASQOP010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 24497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24497
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69697"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(290..937)
                     /locus_tag="VE030_06755"
                     /pseudo
     CDS             complement(290..937)
                     /locus_tag="VE030_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
     gene            complement(981..1736)
                     /gene="surE"
                     /locus_tag="VE030_06760"
     CDS             complement(981..1736)
                     /gene="surE"
                     /locus_tag="VE030_06760"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001489.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HZF23149.1"
                     /translation="MRILLSNDDGYFAPGLAVLAEALSKHAEIIVVAPERDRSGASNS
                     LTLDRPLQVRRSHNGFHYVNGTPTDCVHLAVTGMLDRLPDMVISGINHGANMGDDTIY
                     SGTVAAATEGFLLGIPSIAVSLASKAAGNYPAAAQAVCDIVERFRHRPIAFPVLLNVN
                     VPDVPADQLAGTAVTRLGRRHKSEPVIKSTNPRGETVYWIGAAGDAEDAGEGTDFHAV
                     ANRMVSVTPLQIDLTQYARLESVRAWLKEPALT"
     gene            complement(1867..1943)
                     /locus_tag="VE030_06765"
     tRNA            complement(1867..1943)
                     /locus_tag="VE030_06765"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1907..1909),aa:Pro,seq:ggg)
     gene            complement(1987..2358)
                     /locus_tag="VE030_06770"
     CDS             complement(1987..2358)
                     /locus_tag="VE030_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZF23150.1"
                     /translation="MDSSNHNSSSPKTPLPPIPAKRYFTIGEVSELCGVKPHVLRYWE
                     QEFTQLKPVKRRGNRRYYQHHEVLLIRRIRELLYEQGFTISGARARLDEAAVQLKPSS
                     SRFNSALLRREIQSLLDILRA"
     gene            complement(2324..2644)
                     /locus_tag="VE030_06775"
     CDS             complement(2324..2644)
                     /locus_tag="VE030_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HZF23151.1"
                     /translation="MTLTKADLADLLFEKVGLNKREAKDMVESFFEEVRIALEAGEGV
                     KLSGFGNFQLRDKPQRPGRNPKTGEEIPITARRVVTFHASQKLKAMVEKNYGGQQQPQ
                     QQQS"
     gene            complement(2654..5068)
                     /gene="pheT"
                     /locus_tag="VE030_06780"
     CDS             complement(2654..5068)
                     /gene="pheT"
                     /locus_tag="VE030_06780"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010115709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HZF23152.1"
                     /translation="MQFSEKWLRTLVDPPLTTDALAHLLSMSGLEVESSVSVAPPFTG
                     VVVGRVLSVEKHPSADKLTVCRVDVGSGQPLSIVCGAPNVVAGMKAPCALVGARLPGA
                     SADIPFEIKVATMRGVESRGMLCSARELGLSDDHSGLLVISGDAAPGSDVRAALGLDD
                     KVLTIKLTPNRADCLSVLGVAREVAALTGAPLNPPDTHSVPATNDARFPVRISEPKGC
                     GRFTGRVIRNVNAEALTPEWMKQRLERAGQHSISALVDVTNYVMLELGRPLHVYDLDK
                     LSGGIDVRSGRKGEKLKLLNEQVVEIDESVLAITDASGPVGLAGIMGGDSTKADLETH
                     NIFLEAAFFFPGAIAGRARRYNFVSDASHRFERGVDFNNNVDGIERATRLILDICGGE
                     PGPVDDTVAQLPQRQPVRMRVARAHKVIGVPVGAEDMADIFRRLNLAFRREGTGAEEV
                     FLVTPPSYRFDIEIEEDLIEEVARVYGFERIPAQPPVAPSTMLPLSECRRSLHDLRLQ
                     MAAAEYQEVVNYSFVDASWEADFAGNADPIRLLNPIASQLAVMRSTLIGGLVANVAYN
                     LNRKQERVRAFEVGRAFLRDPAVRDGQLDVAGIRQPVRVAAVAYGTAVEEQWGIPARG
                     VDFFDVKNDLETLIAPRGARFEPVMHPALHPGRGARVMLDDAVIGWIGELHPRWQKKY
                     ELPEPLVAFEVDAEPLLRLPLPRYRELSRFPPVRRDLAVTVDEAAPVQAILDGLLRAK
                     PAIVHAVRVFDVYRGKGIENGKKGLAFRVLLQDTQKTLTDAEVDAAIAGLRKVLEQEY
                     GAKLRQ"
     gene            complement(5107..6105)
                     /locus_tag="VE030_06785"
     CDS             complement(5107..6105)
                     /locus_tag="VE030_06785"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008113575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HZF23153.1"
                     /translation="MDNLEKIVADAKSAFERVADLAHLEQAKARFLGKTGALTEQLKG
                     LGKLPAADRPSAGALINQAKEKVEALLAARRDAIQAQSLEARLAQEALDVTLPGRGRG
                     RGGIHPVIRTWQRIEGIFRSAGFDVADGPEIETDWYNFTALNQPENHPARSMHDTFYI
                     EGGLLLRTHTSPIQVRYARAHKPPIKVIALGRTYRVDSDATHSPMFHQVEGLWIDEYV
                     SFADLKGIYTDFLRRFFETDDLQVRFRPSFFPFTEPSAEIDMAFPSGPLEGRWLEISG
                     AGQVHPNVVRNFGLDPERYIGFAFGSGLERLTMLRYGINDLRLFFENDLRFLKQFN"
     gene            complement(6172..6531)
                     /gene="rplT"
                     /locus_tag="VE030_06790"
     CDS             complement(6172..6531)
                     /gene="rplT"
                     /locus_tag="VE030_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HZF23154.1"
                     /translation="MPRVKRGVTAHARHKKVLAKAKGFRGRRGNVYRIAKQAVMRAGQ
                     YAYRDRRQKKRDFRALWIVRINAAAREFGMTYSTLINGLKKASVEVDRKVLADIAVFD
                     KQAFERIASQAKASLGA"
     gene            complement(6543..6740)
                     /gene="rpmI"
                     /locus_tag="VE030_06795"
     CDS             complement(6543..6740)
                     /gene="rpmI"
                     /locus_tag="VE030_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HZF23155.1"
                     /translation="MPKMKTKSGAAKRFRALGNGRFKRSQANLRHILTKKSTKRKRHL
                     RGTTAVKASDHGHIRAMLPYA"
     gene            complement(6905..7423)
                     /gene="infC"
                     /locus_tag="VE030_06800"
     CDS             complement(6905..7423)
                     /gene="infC"
                     /locus_tag="VE030_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HZF23156.1"
                     /translation="MAQDKDVRINGEITAPEVRLIGIKGEPIGIVSLAQANAMAEELE
                     IDLVEIAPTAKPPVARLMDFGKFKYSEAKKRHEAKLKQKQIQVKEVKFRPGTDEGDYK
                     IKLRNLIRFLEEGDKTKVTLRFRGREMAHQEFGVKLLERVKADLEPYGAVEQFPKMEG
                     RQMVMVLSPKKK"
     gene            complement(7445..9361)
                     /gene="thrS"
                     /locus_tag="VE030_06805"
     CDS             complement(7445..9361)
                     /gene="thrS"
                     /locus_tag="VE030_06805"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HZF23157.1"
                     /translation="MPIVRLPDGSQKTFDKPVTVAEAAAAIGAGLARAALAGRVDGKL
                     VDTSYLIDRDADLAVVTDKDPDGLEILRHSTAHLLAHAVKELYPDAQVTIGPVIEDGF
                     YYDFAYKRPFTPEDLAAIEKRMAEIAKRDVKVERRVWNRAEAINFFKNQGEHYKARII
                     EAIPGDETISLYTQANFTDLCRGPHVPSTGKLKVFKLLKVAGAYWRGDSKNEMLQRVY
                     GTAWAKKEDLDAYLFRIEEAEKRDHRKLGRQLDLFHLQDEAPGMVFWHPKGWAIWQQV
                     EQYMREILSRHGYREVRTPQLMDRVLWEKSGHWDNYRELMFTTESEKREYAVKPMNCP
                     GHVQIFNQGIKSYRDLPLRLAEFGACHRNEPSGALHGIMRVRGFTQDDAHIFCTEDQV
                     QAEASAFIDLLQKVYADFGFSEILIKLSTRPAKRIGSDETWDRAEAALKAALDAKGLR
                     WDLNPGEGAFYGPKIEFSLKDSIGRVWQCGTLQIDFELPNRLGAEYIAEDNSRKTPVM
                     LHRAILGSMERFIGILIENHAGAMPLWLAPQQVVVMNISEHQTEYAAGVAERLKAAGL
                     RAFADLRNEKITYKIREHSLQKLPYQVIVGDKEVAANAVAVRTRGGADLGQMSFDAFV
                     ARLRSELPHAGSTA"
     gene            complement(9506..9582)
                     /locus_tag="VE030_06810"
     tRNA            complement(9506..9582)
                     /locus_tag="VE030_06810"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9546..9548),aa:Val,seq:gac)
     gene            complement(9629..11806)
                     /locus_tag="VE030_06815"
     CDS             complement(9629..11806)
                     /locus_tag="VE030_06815"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HZF23158.1"
                     /translation="MVKVREITSDTASAEWLEVVTVRLSQAERELVVRADLWAQEYYA
                     DSRHPAGTRWIEHVRSAVGILSALRVDGEAVGATLLLGVPVSSRAQREALAAQFGPAI
                     AALVEGVGSMAQIQVLRGRVESGPRQADRAAQLESLRKMLLAMVQDVRVVLIKLADQV
                     QLLRDLAATGDARARENAAGDTMDLFAPLANRLGVWQIKWELEDLAFRCLEPHTYKNL
                     ARQLDEKRVDRESYITNLVQLLRAELAAAGIAAEVAGRPKHIYSIWRKIQSKGGGLEN
                     LFDVRALRVLVNDVKACYAVLGLVHNLWTPVPREFDDYIAKPKANDYRSLHTAVVGPE
                     GKVLEVQIRTHEMHQHAEFGVAAHWRYKEAVKGDTTFDDRIAWLRRILDWRDELADAG
                     ELAEYFKTELFQDSVYVVTPQGRVIGLPRGATPVDFAYHVHSELGHRCRGAKVNGQIV
                     PLTYALSNGQRVEIIAAREGGPSRDWLNPALGYLKSNRARAKVRQWFNSQQLEEATTR
                     GRAVLERELHRLGKTGQNLDELAARLNFTKVEELFAALGRAEVTPRQLQSALGADDVP
                     VAPAVETRVPSQPRPGAGGILVVGVDKLLTVLARCCKPAPPDPIVGFVTRGRGVTVHR
                     RDCSNVARLATERLIAADWGKTGEARFQVDVEIIAGAHPALLRDILDIFTREKVRVIT
                     SKAVSRDLNARIALTLEVEGLPQLKRLLAQVRDVPGVESARRR"
     gene            complement(11823..14168)
                     /locus_tag="VE030_06820"
     CDS             complement(11823..14168)
                     /locus_tag="VE030_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HZF23159.1"
                     /translation="MIVNVAALTAGAWWLQQQPLLPDLTGALVLVPLAALWLLAARRK
                     GAAAGLAARAAALAASAIAGFFWSAAIGHWSLAHALPEQWEGRDIELSGVIAEMTQPA
                     ERGVRFAFDVERAHTPGAEVPPRISLTWYQSGDTAQAPDLHPGQRWRLSARLRRPHGN
                     ANPDGFDFEAWMLERGIRAVGYVRADAPAALMAARVNRPAYVLERAREAVRARLLRAL
                     DGRPYAGVIVALAVGDQRAIPPSQWRVFTRTGVNHLMSISGLHITMVASLAYLLVLRL
                     WRQSETLTLRLPAARAAAAAGLAAALAYAALSGFAVPAQRTVFMLAIVAVALWLGWMT
                     RPLAVLAAAAALVIVLDPMAVISAGFWLSFGAVAVIMLAGGGRIGRSSWLGTWARTQW
                     AVTLALVPLLLAMFQQVSLVSPIANAVAIPLVSLVVVPLTLLAAVLPLDWLAHAAHAV
                     MALCMIVLDTLGRVPDAAWQQHAPPSWAVPLAMIGALWILLPRGFPARWVGAALMLPL
                     FLWLPPRPSPGELWLTVLDVGQGLAVVVRTHGHALLYDTGPAFSDQVSAGERLVVPYL
                     RASGVRGLDGIIVSHDDLDHSGGALAVLQAMPVQWLSSPLSDVHPISEAAAHGRRCSA
                     GEGWEWDGVAFEMLHPDAESYNEFDLKDNDRGCVLRIVSPYGAVLLPADIERRSEIAL
                     LRDSPGRLSADVLIAPHHGSRTSSTPEFVRAVAPRLVVFSVGYRNRFGHPHPAVVRRY
                     REQGSDMARTDLAGAVLVKMGSSGIETSGWRQLERRYWQGR"
     gene            14201..14761
                     /locus_tag="VE030_06825"
     CDS             14201..14761
                     /locus_tag="VE030_06825"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HZF23160.1"
                     /translation="MPRKFFRKYLPSHESVRQNRHIRFFGTALHHPNLWHLNRKSVAG
                     GTAVGLFCGLVPGPLQMLSAALLAILFRVNLPVSVILTWYTNPFTIVPLYYIAYKLGM
                     FVTGGNGHVLPGGELDLLALPIMEWIPALYHWLAAMGKPFALGLFLLALILAVAGYLL
                     VIAAWRVHVTLAWNKRRRMRIFAARK"
     gene            14871..15488
                     /locus_tag="VE030_06830"
     CDS             14871..15488
                     /locus_tag="VE030_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HZF23161.1"
                     /translation="MTPSYWNDATRDLASRDSVLKILIRNLEGPQLQSRSDAFTTLAR
                     SIIGQQISVQAAESVWQKLLESVGSLGPATIVRMDPSKLRACGLSSKKSTYLIDLAEN
                     FLDGSLELERWSDHDDEQLIAELTRVKGIGRWTAEMFLIFFMMRPNILPVDDIGLQRA
                     MGLHYNNGKPLSKLKMRQIATEWQPWRSVATWYLWRSLDPIPVEY"
     gene            complement(15634..16314)
                     /gene="lolD"
                     /locus_tag="VE030_06835"
     CDS             complement(15634..16314)
                     /gene="lolD"
                     /locus_tag="VE030_06835"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HZF23162.1"
                     /translation="MSEVVLSCRNLQKTFHQGEYAVPVLLGIDIDVARGETVAIVGAS
                     GSGKSTLLHLLGGLDAASAGEVTVLRHLLDSLNEKEKGRLRNRALGFVYQFHHLLPEF
                     TALENVAMPLLIRRLDSKEAGKRAAGILQRVGLSARMTHTPGELSGGERQRAALARAL
                     VTEPACVLADEPTGNLDRHTAEGMLDLMLELNRDLATSFVVVTHDPTIAAKMQRTLRL
                     EDGKLREA"
     gene            complement(16307..17554)
                     /locus_tag="VE030_06840"
     CDS             complement(16307..17554)
                     /locus_tag="VE030_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HZF23163.1"
                     /translation="MLTPYELFVGLRYTRAKRRNHFISFISITSMLGVALGVAALIVV
                     LSVMNGFQKELRTRILGVASHVQIMGLDGKLSDWRKIAAQAQSHSEVIAAAPYITAQG
                     LISHHGSVQGTLVRGVLPAAEAKVADVGQHMRSGSLDDLRPGRFGIVLGVDLAMSVGA
                     RVGDKIVLIAPQGQVTPAGILPRLKQFEVVGIFEVDMAEYDAGLAFMHLEDAQKLYRL
                     EDKVSGVRLKLHDLFKAPRVARDLMRTLSSDVYATDWTRSHANFFRAVQIEKRVMFII
                     LVLIVAVAAFNIVSTLVMAVTDKRADIAILRTLGAAPGEIMRIFIIQGALIGAVGMIA
                     GVICGVLLAANIEVVIPAIEHVLGVQFLAKDVYYISDLPSDLHLQDVVVIGLTSFGLS
                     LLATMYPSLRASRVNPAEALRYE"
     gene            17705..18754
                     /locus_tag="VE030_06845"
     CDS             17705..18754
                     /locus_tag="VE030_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23164.1"
                     /translation="MHTVTLFVPSLLWPETGDPDAYPGPRLPALETLLSRGSAAESAC
                     ENEPAWLCERFGVQKQIDWPVAPLAFAGDGGEAGADYWLCADPVHLRLHNDGLILLAP
                     ESLELAEGESRELTGALNRQFETEGFVFHAPHPRRWYLKLPQAPKIRTVTLKQAIGRD
                     VNRLLPEGEERLRWHRIFNEVQMLLHAEPVNAAREDRGVAVANSLWFWGGGTLPASRS
                     SFDAMQSDDPLAVGLARLAGIISAPLPVDGGFAAGNNTLVDLRDAEREFMRGNAAGWR
                     HALETLETRWFAPLFDCLRAGRIGKAVIATVADGRRYEWSVTRGVRWQIWKRPRPLAH
                     HAGNVLHRHDPARLA"
     gene            18751..20454
                     /gene="recJ"
                     /locus_tag="VE030_06850"
     CDS             18751..20454
                     /gene="recJ"
                     /locus_tag="VE030_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HZF23165.1"
                     /translation="MITRRIVTRSVPQPAFDSLCANGVHPVLARAYAARGVSTPRQLD
                     TSFNGLAPAERLKNCVAMARLLADAIAARQRLLIVADYDADGATACALGMIGLQSFGA
                     QVDYLVPNRFEYGYGLTPEIVALAAARRPDFLITVDNGIASIDGVEAARKLGIRVLIT
                     DHHLPTAQLPAAACIVNPNQPGCDFPSRNLAGVGVMFYVLLALRAELRARGWFGGRDE
                     PNLADLLDLVALGTVADVVRLDDNNRILVDQGLKRIRAARARPGITALLRVAGRDPAR
                     VVAYDLGFVLGPRLNAAGRLTDMSVGIECLLARDSDRAAELAAQLDALNRERRDIEAG
                     MQETALALIERIAPADAYSLSLYDETWHQGVVGILAARLRERFNRPVIAFAPGGNGEA
                     KGSGRSIPGLHLRDALDLVSKRHPGLILRFGGHATAAGLTVRSADMAAFENAFEQTVR
                     GLLTPADLEHVIETDGGLEPDDVSLPFAELLAAQVWGQGFPPPSFFDEFEVTEQKVVG
                     SRHLKLRLRRRLAEQAFNAILFGRSAPLPSRVSAVYRVHVNEFNGMRSVQLVLDHWQA
                     G"
     gene            20472..21731
                     /locus_tag="VE030_06855"
     CDS             20472..21731
                     /locus_tag="VE030_06855"
                     /inference="COORDINATES: protein motif:HMM:NF024592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HZF23166.1"
                     /translation="MDFYWLQGTELERLPAFAISLAIGLLIGLERERHPDTNAGLRTF
                     ALTALFAAVAALLSDETGSPAILIIGLVIVAAMIVTANARDREQHDPGTTTVIALLLC
                     YGLSVMTWFEYQTLAVMLAIVTTSLLYFKAELRGIAERLERRDLISILQFAVLMFIVL
                     PILPNRDFGPHGALNPYQIWTMVVLISGVSLAGYVAVRLVGLRYGAPLLGLLGGLVSS
                     TATTLIYARHARAQERLSGLAVLVIVLANLVLLVRLAVLTAFVAPGLLGTLLPLLGGG
                     LLLGLASLGYLYARQGRDNTAPIPDIRNPAELRTALTFGAIYALVLLLAAWLSGLAGA
                     AGLYAVALIAGLTDVDAITLASLRLFSVGTVEAHEVAIAVVLAIGANIVFKLGMVLIV
                     GGQSLFARCAGSMAAVAAGMIVALLFT"
     gene            21809..22913
                     /gene="prfB"
                     /locus_tag="VE030_06860"
     CDS             join(21809..21889,21891..22913)
                     /gene="prfB"
                     /locus_tag="VE030_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435212.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HZF23167.1"
                     /translation="MEAERLNAIEKQLDDLDTRAKELRRYLDFDGKRNRLIEVVKLTE
                     DPAFWNDAKRAQDLGRERKNLESLVTTLEKLDQGLKDARELFQMARSENDDATLNAVA
                     DDVAGLERIVADMEFRRMFSNPMDPNNCFLDIQSGSGGTEAQDWASMLERMYLKFCER
                     RGFKVEVLEESSGEVAGIKSATLKVNGDYAYGTLRTESGVHRLVRKSPFDSNARRHTS
                     FASVFVYPEVDDTIEIAINPADLRVDTFRASGAGGQHINKTDSAIRITHVPTGIVVQC
                     QNDRSQHRNRAEAMAMLKARLYELELRKRNEEKQAMEDSKTDIAWGHQIRSYVLDQSR
                     IKDLRTNVEIGNTQAVLDGALDPFIEASLKQGV"
     gene            23035..>24497
                     /gene="lysS"
                     /locus_tag="VE030_06865"
     CDS             23035..>24497
                     /gene="lysS"
                     /locus_tag="VE030_06865"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HZF23168.1"
                     /translation="MEEKNQLPIVDTNQIVEERRAKLRELRAQGNAFPNDFRRRHLAA
                     DLHRAYGATPNEELEPLKAEATVAGRMMLKRVMGKASFATIQDMSGRIQIYVTNDVTG
                     EPVHTAFKHWDLGDIVGATGTLFKTRTGELTVKAAGVHLLAKSLRPLPEKFHGLADQE
                     AKYRQRYLDLITNPEAQRVFKTRSRMIQAMREFFVARGYLEVETPMMQPIPGGAAARP
                     FVTHHNALDMDLYLRIAPELYLKRLVVGGIEKVFEINRNFRNEGISTRHNPEFTMLEF
                     YEAYQDYNYLMGLTEELLRQVAREVLGTTTFSYQGETIDLGKRFDRLTMAEAIAKYNP
                     EYPMAELGKTAFLRTALDAFKGEVFSTDGLGLLQLKLFEQTTEDKLIQPTFIVAHPTD
                     VSPLARRNDANPAVTDRFELFVTGREMANGFSELNDPEDQAERFSEQARAKEAGDEEA
                     MYYDADYVRALEYGLPPTAGEGIGIDRLVMLFTDSPS"
BASE COUNT         4451 a         8025 c         7540 g         4481 t
ORIGIN      
        1 tccgtggctg cggaaacccg tgtctccgtt ttgtggacag tcgagacggt tggcgcccct
       61 gcaggggggg gtgcagacgt ctggggtatg ccggcagccg gagcggtggc cacaggtgcg
      121 gctgtcgtgc gtatggcgac cggagctacg gccggcgcgc gggacgcatt cggcgaacgc
      181 tcgagcaccg gcgcggcagt aggcgtggat gcgcaacccg cgccgagcgc ggcaaccaat
      241 gctgcaagca tgagcggccg cgaaaattcc ccgcagcgga aaacgatagt caagccgaaa
      301 cccccgcaat cagcggaacg aaattgacgt cgtccagcac cgactggctg aatccgtcgc
      361 ggccgcgctc gaccagcacc agacgctgct cgcgcgtgcc caacggcagc acgaggcgcc
      421 cgccctccac cagttgagca gccagcgcct cgggtatgtg cgtcgccgcc gccgccatca
      481 cgatggcgtc gaacgggccg ccgtccttca ggccgagatg accgtcgcca tgcctgagtt
      541 tgatgttgcg aattccgagt tcgcgcaatc tcacccgcgc cttggacaac aggccggcga
      601 gacgctcgat cgaataaact tcgcgcgcca cccgcgataa gaccgcggcc tggtaaccgc
      661 agccggtgcc gatctcgagc actttgccga gcgcgcgacc agcgcgcgcc agttcgacca
      721 tgcgggcgac agcgaacgga ttggagatgg tctggccgaa accgatgggt agcgatacgt
      781 catcgtaggc acggcttgcc agcgcctcgt cgacgaacat gtggcgaggg acatccatca
      841 tcgcacccag caccacttcg tcctttacgc cctggtcgcg caggcgctcc accatgcgca
      901 tgcgggtgcg ttgggaggtc atgccgatgc cgctgatcct gctgttcata tcgtgtcgaa
      961 atggccgccg cgcaaatatt ctaggtgagt gccggttctt tcagccaggc gcgcacgctt
     1021 tcgagcctgg cgtactgcgt gagatcaatt tgcagcggcg tcaccgaaac catccggttc
     1081 gcgaccgcgt gaaagtccgt gccctcgcct gcatcctcgg catcgcctgc ggcgccgatc
     1141 cagtacaccg tttcgccgcg cgggttggtg gacttgatga ccggctcgga cttgtgccgc
     1201 cgtcccagcc tggtcactgc cgttcctgcc agttgatcgg ctggcacatc cgggacattg
     1261 acgttgagca ggaccggaaa cgcaatcggt cggtggcgaa aacgctcgac tatgtcgcac
     1321 accgcctgcg cggcggccgg atagttgccc gccgctttgc tcgccagcga caccgcgata
     1381 gagggaatgc ccaggagaaa tccttccgtt gccgccgcca ccgttccgga gtatatggtg
     1441 tcgtccccca tgttggcacc atgattgatg ccggaaatca ccatatccgg gagccggtcc
     1501 agcatgccgg tcacggcgag gtgtacgcag tcggtgggcg taccgtttac gtagtgaaag
     1561 ccgttgtgcg agcgcctgac ctgcagcggg cgatcgaggg tgagcgaatt gctggcgccg
     1621 ctgcggtcgc gctcgggcgc gaccacgatg atctcggcat gtttcgacag cgcctcggcg
     1681 agcacggcga ggccgggcgc gaagtacccg tcgtcgttgc tcaggagaat gcgcatcgga
     1741 gaaaaaggct gcggatcagc cttcgatcga gggcgccccg cgaaataaca gacctggaaa
     1801 ccggatcttc ccgtccgcgc atctttctcg cgagcccgcg ggcccctcgt ctttaccgcg
     1861 atagtttggt cggggcgaga ggatttgaac ctccgaccac ctgcacccca tgcaggtacg
     1921 ctaccaggct gcgctacgcc ccgaatcgag agcggattat agcagaggcc ccgcgcgcgg
     1981 aacgtctcag gcgcgcagga tgtcgaggag actctgaatt tcccggcgca atagcgcgga
     2041 gttgaatctg gaggaagacg gtttgagttg cactgcagcc tcgtccagcc gcgcccgcgc
     2101 gccgctgatg gtgaacccct gctcgtacag cagttcgcgg atgcgccgga tcagcagcac
     2161 ttcatggtgc tgataatagc gccgattgcc gcgccgctta accggcttca actgggtaaa
     2221 ctcctgctcc cagtagcgca gcacgtgtgg tttgaccccg catagctcgc tgacctcacc
     2281 gatggtgaag taacgttttg cgggaatcgg gggcagcggg gtcttaggac tgctgctgtt
     2341 gtggttgctg ctgtccaccg tagttctttt ccaccatcgc cttcagtttc tggctggcgt
     2401 gaaaggttac gactcgacgc gccgtaatcg gaatttcctc gccggttttg gggttgcggc
     2461 ccggtcgctg cggcttgtcg cgcaactgga aattaccgaa accggacagc ttcacgccct
     2521 cccccgcctc gagtgcgatg cgcacttcct cgaagaacga ctccaccatg tctttggctt
     2581 cgcgcttgtt caaaccgact ttctcgaaca acagatccgc caggtctgct ttggtcagtg
     2641 tcatgacttc accttattgt ctcagcttgg caccgtattc ttgttccagg accttgcgca
     2701 gacccgcaat cgcggcgtcc acctcggcat ctgtcagagt cttttgagta tcttgcaata
     2761 acacacggaa agcaagacct tttttcccgt tttcgatacc cttgccgcgg tacacgtcga
     2821 acacccgaac ggcgtgaacg atggccggtt tcgcccgcaa aagcccgtcc agaatggctt
     2881 gcaccggggc agcctcgtcc acggtgaccg ccaggtcgcg gcgcaccggg ggaaatctgg
     2941 acagctctcg ataccgcggc agcggtagcc gcagcagcgg ttcggcatcg acttcgaaag
     3001 caaccagcgg ctcaggcaac tcgtacttct tctgccatct cgggtgcaat tcgccgatcc
     3061 agcctatgac cgcgtcatcc agcataaccc gggccccacg cccggggtgc aacgccgggt
     3121 gcataaccgg ctcgaagcgc gcgcccctgg gtgcgatcag cgtttccagg tcgttcttga
     3181 cgtcgaagaa atcgacgccg cgcgccggaa tgccccactg ttcttcgacc gcagtgccgt
     3241 acgcaacggc cgccacgcgc accggctggc ggatcccggc gacatccagt tggccgtccc
     3301 tgacggcggg atcgcgcagg aacgcccgcc ccacctcgaa cgcacgcacc cgctcctgtt
     3361 tgcggttcag attgtaggca acgttcgcca ccagcccgcc gatcaacgtg gaacgcatga
     3421 ccgccagctg gctggcgatc gggttgagca accggatcgg atcggcattg ccggcaaaat
     3481 ccgcctccca ggaagcatcc acgaaactgt agttgaccac ttcctggtat tcggcggccg
     3541 ccatctgcag ccgcaggtcg tgcagcgagc gccggcactc ggagagcggc aacatcgtac
     3601 tcggggccac cgggggctgc gcgggaatgc gttcgaagcc atacacccgc gccacctcct
     3661 cgatcaggtc ctcctcgatt tcgatgtcga agcggtacga aggcggggtg accagaaaga
     3721 cctcctcggc gcccgtgccc tcgcgccgga acgcgagatt cagacgcctg aagatatccg
     3781 ccatgtcctc cgcgccgacc ggcacgccga tgaccttgtg cgcacgagcc acccgcatac
     3841 gcaccggttg gcgctgcggc aactgcgcca ccgtgtcgtc caccggaccg ggctcgccac
     3901 cgcaaatatc aagaatcagc cgggtcgcgc gctcgatgcc gtcgacgttg ttgttgaagt
     3961 cgacgccgcg ttcaaagcgg tgcgaggcgt cgctgacgaa gttgtagcgc cgcgcgcgtc
     4021 cggcgatcgc ccccggaaag aagaacgcgg cctcgaggaa gatattgtgg gtttccaggt
     4081 ccgccttggt gctgtccccg cccatgatgc ccgcaagtcc gaccggcccg ctggcatcgg
     4141 tgatcgccag cacgctctcg tcgatctcca cgacctgctc gttgagcagc ttgagtttct
     4201 cgcccttgcg gccggagcga acgtcgatgc cgccgctgag cttgtccagg tcgtagacgt
     4261 gcagcggccg gcccagctcg agcatcacgt aattggtgac gtccaccagc gccgaaatcg
     4321 aatgctgccc ggcacgctcc agccgctgtt tcatccattc cggcgtgagc gcttcggcat
     4381 tgacgttgcg gatcacccgc ccggtgaagc gtccgcatcc tttgggttcg gatatccgca
     4441 ctggaaacct ggcgtcgttc gtggcgggta ccgagtgcgt gtccggtggg ttcagcggcg
     4501 caccggtcag cgccgcaact tcgcgtgcca cgccaagcac tgacaggcag tcggcgcgat
     4561 tgggggtgag cttgatcgtc agcaccttgt catccagccc cagcgccgcg cgcacgtcgc
     4621 ttccgggcgc ggcatccccg gaaattacca gcagaccgct gtgatcgtcc gacagtccga
     4681 gttcgcgcgc cgagcacagc atgccgcggc tttccactcc gcgcatggtc gcgaccttga
     4741 tttcgaacgg tatgtctgcc gatgcacccg gcagcctggc gccgaccagc gcacacggag
     4801 ccttcatccc ggcgaccacg ttgggcgcac cgcagacaat gctgagcggc tgaccgctgc
     4861 cgacgtcgac ccggcacacc gtcagtttgt ccgcactcgg gtgtttctcg acgctgagca
     4921 cgcggcccac caccactccg gtgaacggcg gcgccacgct cacgctactc tccacttcca
     4981 ggcccgacat gctcagcaga tgcgcgagcg catccgtggt caaaggtgga tcgaccagtg
     5041 tgcgcaacca tttttccgag aactgcattc ttgtctctta gtgaatcttc ctggcgtctg
     5101 ggcgattcaa ttgaattgct tgaggaagcg cagatcgttt tcgaagaaca accgcaggtc
     5161 gttgatgcca tagcgcagca tggtcagccg ctcgagcccg ctgccgaagg cgaatccgat
     5221 gtagcgctcc gggtcgaggc cgaagttgcg cacgacgttc ggatgcacct gcccggcccc
     5281 ggaaatttcc agccagcggc cctccagcgg cccgctggga aacgccatgt cgatttcggc
     5341 ggagggctcg gtaaacggaa aaaacgatgg gcggaagcgc acctgcaggt cgtcggtctc
     5401 gaagaaacgg cgcaggaaat cggtgtatat gcccttcaga tccgcaaaac tgacgtattc
     5461 gtctatccac agcccctcga cctggtgaaa catcggcgag tgggtcgcat ccgaatccac
     5521 gcggtaggtg cgtcccagcg cgatcacctt gatcggcggc ttgtgcgcgc gcgcgtaacg
     5581 cacctggatc gggctggtgt gggtacgcag cagcaatcct ccctcgatgt agaaggtgtc
     5641 atgcatcgag cgggcgggat ggttctcggg ttggttgagc gcggtgaagt tgtaccagtc
     5701 ggtttcgatt tccgggccat cggcgacatc gaatcccgcc gaacgaaaaa tgccttcgat
     5761 gcgctgccac gtgcggatga ccgggtgaat gccgccacgg ccgcggcctc gccccggcag
     5821 cgtgacgtcc agggcttcct gtgccaggcg ggcttcgagc gactgtgctt ggatggcatc
     5881 acggcgcgcg gcgagaagcg cttctacctt ctcctttgcc tgattgatca gcgcacctgc
     5941 gctcgggcgg tccgccgccg gcagcttgcc aagacccttc aattgttcgg tgagcgcgcc
     6001 ggtcttcccc aggaagcgcg ccttcgcctg ctccagatgt gcgagatctg caacccgctc
     6061 gaacgcggat ttggcatcgg cgacgatctt ttccaggttg tccatgggtc actgccgtcc
     6121 ggtgaataaa aaaaggaggc cctgaagcct ccttttcgct gcttgtccga atcaggcacc
     6181 gaggctggct ttcgcctgac tcgcaatcct ttcaaaagcc tgtttgtcga acacggcaat
     6241 gtcggcgagc accttgcggt ccacctcgac cgatgccttc ttcaggccgt tgatcagcgt
     6301 gctgtaggtc atgccgaact cgcgagctgc ggcattgatc cgcacgatcc acagcgcgcg
     6361 gaaatcacgc tttttctggc ggcggtcacg gtaggcatat tggccggccc gcatcaccgc
     6421 ctgtttggcg atacggtaga cgttgccgcg gcggccgcgg aaacccttgg ctttggcgag
     6481 gactttcttg tgccgcgcgt gcgcggttac tccacgtttg actctgggca tggctgtccc
     6541 ccttacgcgt acggaagcat ggcacggata tgcccgtggt ccgatgcctt cacggcagtc
     6601 gtgccgcgca gatggcgctt gcgcttggtc gacttcttgg taaggatgtg gcgcaggttc
     6661 gcctgcgagc gtttgaagcg gccgttaccc agcgcgcgga agcgcttggc tgcgccgctt
     6721 ttggtcttca tcttgggcat gaatcaactc ctttgtttta gcctgacgcc gggtggccgc
     6781 aggcggcgct ttggaacccg gacatcactt gtttgacaac ggtgactggt gatgcgtaat
     6841 cggtaattgg caaaaacctt tcgccgatcc cactggacac caatcactaa ttactaatca
     6901 cgaatcactt tttcttgggg gacagcacca tgaccatctg ccggccctcc atcttcggaa
     6961 actgttcgac cgcgccgtag ggctcgaggt ccgccttgac acgctccagc agcttgacgc
     7021 cgaactcctg atgcgccatt tcgcggccgc gaaagcgcaa cgtcaccttg gttttgtcgc
     7081 cttcttcgag gaaacggatc aggttgcgca gcttgatctt gtaatcgcct tcgtcggtgc
     7141 cgggccggaa cttcacttcc ttgacctgga tctgtttctg tttgagcttt gcctcgtgcc
     7201 tcttcttggc ttcgctgtac ttgaacttgc cgaaatccat cagccgggca accggcggtt
     7261 tggcggtagg tgcgatttcc accaggtcga tttccagttc ctccgccatg gcattcgcct
     7321 gggcgagact gacaatcccg attggctcac ccttgatgcc gatgagccgg acctccggcg
     7381 cagtgatctc gccgttgatc ctgacgtcct tgtcctgagc tatgagcaga cctccatcaa
     7441 aaaattaagc cgtacttccc gcatggggca actcggatct cagccgtgcg acgaaggcgt
     7501 cgaacgacat ctggccgaga tcagcacccc cccgggtacg cacggcaacg gcgtttgcag
     7561 cgacctcctt atccccgacg atcacctgat aaggcagttt ctgcaaacta tgttctcgaa
     7621 ttttataggt tattttctcg tttctcaagt ccgcaaaggc gcgtagccca gccgccttaa
     7681 ggcgctcggc caccccggcg gcatattccg tctgatgctc ggagatgttc atgaccacca
     7741 cctgctgcgg agccagccac agcggcatcg ccccggcgtg gttttcgatc aggatgccga
     7801 tgaagcgctc catcgatccc aaaatcgccc ggtggagcat aaccggcgtc ttcctgctgt
     7861 tatcttcggc gatgtattcg gcgccaagcc ggttggggag ctcgaaatcg atctgcaggg
     7921 tgccgcactg ccacaccctg ccgatcgagt ccttgagcga aaactcgatc ttcggaccat
     7981 agaacgcgcc ctcgccgggg ttgagatccc atctaaggcc tttggcgtcc agcgccgctt
     8041 tgagcgccgc ttcggcccgg tcccaggtct cgtccgaacc gatgcgtttg gccggccggg
     8101 tcgacagctt gatcaggatc tcgctgaaac cgaagtcggc gtacaccttc tgcagcaggt
     8161 cgatgaacgc cgacgcctct gcctgcacct gatcctcggt acagaaaata tgcgcatcgt
     8221 cctgggtgaa tccgcgcacc cgcatgatcc cgtgcaaggc cccggacggc tcgttgcggt
     8281 ggcaggcgcc gaactcggcc agacgcaaag gcaggtcgcg gtagctcttg ataccctggt
     8341 tgaagatctg cacgtgcccc gggcagttca tcggcttcac cgcgtactcg cgcttctccg
     8401 actcggtagt gaacatcagt tcgcgatagt tgtcccagtg cccggatttt tcccagagca
     8461 cgcggtccat cagctgcggg gtgcgcacct cccggtagcc gtgccgcgac aggatttcgc
     8521 gcatgtactg ctcgacctgc tgccagatgg cccagccctt gggatgccaa aacaccatgc
     8581 cgggcgcctc gtcctgcagg tggaacaggt cgagttgcct gccgagcttg cggtggtcgc
     8641 gtttttccgc ctcttcgatc cggaacagat aggcgtcgag atcttccttc ttcgcccagg
     8701 ctgtgccata gacccgctgc agcatttcgt tcttcgagtc gccgcgccag tacgcaccgg
     8761 cgaccttgag cagcttgaag accttgagct tgccggtgga gggcacgtgc gggccgcggc
     8821 acaggtcggt gaagttcgcc tgggtataaa gggaaatcgt ctcgtcgccg gggattgcct
     8881 cgatgattcg cgccttgtag tgttcgccct ggttcttgaa aaaattgatc gcctcggcgc
     8941 gattccatac ccgccgctcg accttgacgt cgcgcttggc gatttccgcc atgcgtttct
     9001 cgatcgcggc aaggtcctcg ggggtgaagg ggcgcttgta ggcgaaatcg tagtagaagc
     9061 cgtcctcgat gaccgggccg atggtgacct gcgcgtcggg atagagttcc ttgaccgcgt
     9121 gcgccaacag gtgcgcggtc gagtggcgca ggatctccag cccgtcggga tccttgtcgg
     9181 tgaccaccgc aaggtcggcg tcccggtcaa tcaggtacga ggtgtcgacc agcttgccgt
     9241 cgacgcggcc ggcgagcgcg gcacgcgcca gtcccgcgcc gatcgcggcg gccgcctcgg
     9301 cgactgtgac cggcttgtcg aaagtctttt gcgaaccgtc tggcaatcgg acgatgggca
     9361 tggttggtgg tctgaatcgg tgtaaaaaaa aagtgcggcc gaaccgcact ttttcgtcaa
     9421 aggaaataca ggttcagcga cgataccgca tcggcccggt cgtgcaagtt cggataccca
     9481 ctttgcctct gcttcccgtg gaagttggta ggcgcgattg gactcgaacc aacgaccccc
     9541 accatgtcaa ggtgatgctc taaccagctg agctacgcgc ctaatgaagg gcggattcta
     9601 gccgaaagga taagcccgtt caaacttctt atcggcgtcg cgccgactcc acgccgggaa
     9661 cgtcccggac ctgcgccagc aggcgcttga gttgcggcaa gccctccact tccagagtga
     9721 gcgcgatcct ggcgttgaga tcgcggctca ccgccttgct ggtgatgacc cgcactttct
     9781 cgcgcgtgaa aatatcgaga atgtcgcgca gcagcgcagg gtgagcgccg gcgatgatct
     9841 ccacgtccac ctgaaagcgg gcttccccgg tctttcccca atcagccgcg atgaggcgtt
     9901 cggtcgccag gcgcgcgacg ttgctgcaat cgcgccggtg caccgtcacg ccgcgcccac
     9961 gtgtgacgaa accgacgatg gggtccggtg gcgcgggctt gcagcagcgt gccaggacgg
    10021 tgagcagttt gtccacgccg acgaccagga ttcctccagc gccgggcctc ggctgcgacg
    10081 gcaccctggt ctcgacggcc ggtgcaaccg gaacgtcatc tgcgccgagc gcggactgca
    10141 gttgccgcgg cgtcacctcg gcgcgcccca atgctgcaaa caactcctcc actttcgtga
    10201 agttcagccg tgccgccagt tcatcgaggt tctggccggt cttgccgaga cgatgcagct
    10261 cccgttccag cacggcccgg ccgcgggtgg tcgcctcctc gagctgctgc gaattgaacc
    10321 actgccgaac cttggcgcgc gcgcggttgc tcttgaggta gccgagcgcg ggattgagcc
    10381 agtcgcgcga cgggccgcct tccctggcag cgatgatttc tacgcgctgg ccgttggaca
    10441 gcgcgtaggt cagcggcacg atctggccgt tgaccttggc gccgcggcag cgatggccga
    10501 gctcggagtg cacgtgatag gcgaagtcga cgggcgtggc gccgcgcggc aggccgatta
    10561 cccgtccttg tggcgtcacc acgtagacgc tgtcctggaa cagctcggtc ttgaagtatt
    10621 cggccagctc gcccgcgtcc gcgagttcat cgcgccaatc gagtatgcgg cgcagccagg
    10681 cgatgcgatc gtcgaacgtg gtgtcgccct tcacggcttc cttgtagcgc cagtgtgcgg
    10741 cgacaccgaa ctcggcgtgc tggtgcatct cgtgagtgcg gatctgaact tccagcacct
    10801 tgccttccgg ccccacgacc gcggtatgca acgagcggta atcattggct ttcggcttgg
    10861 cgatgtaatc gtcgaactcg cgcggcaccg gggtccacag attatgcacc aggccgagca
    10921 cggcgtagca cgccttgacg tcgtttacca gcacccgcag cgcacgcacg tcgaacagat
    10981 tttccagccc cccacccttg ctctgaatct tgcgccagat gctgtagatg tgtttcggcc
    11041 gccccgcgac ttcggcggcg atgccggcgg ccgcgagttc cgcgcgcagc aactggacca
    11101 ggttggtgat gtagctctcg cgatccacgc gtttctcgtc cagctgacgc gcgagattct
    11161 tgtaggtgtg cggctcgagg cagcggaacg ccagatcctc aagctcccac ttgatctgcc
    11221 acacgccaag ccggttggcg agcggcgcga acaggtccat ggtatcgccg gcggcgttct
    11281 cgcgagcccg cgcgtcgcca gttgccgcga gatcccgcag caactgcacc tgatcggcga
    11341 gcttgatcag caccacccgc acgtcctgga ccattgccag cagcatcttg cgcagcgact
    11401 cgagctgcgc cgcgcggtcg gcctggcggg gaccggactc gacccgtccg cgcagcacct
    11461 ggatctgcgc catgctcccc acgccttcca ccagcgcggc aatcgccggc ccaaattgcg
    11521 ccgccagagc ttcgcgctgc gcgcgggacg acaccggcac accgagcagg agtgtcgcgc
    11581 caacggcttc cccatcgacg cgcagcgcgc tgagtatgcc gacggctgag cgcacgtgct
    11641 cgatccagcg cgtacccgcc ggatgccggc tgtcggcgta gtattcctgc gcccagaggt
    11701 cggcgcgcac caccagttcg cgctcagctt gcgacaggcg caccgtcacc acctccagcc
    11761 attcggcgga tgcggtatcg ctcgtaattt ccctgacctt gaccatatat cgataggcga
    11821 aatcaacgcc cttgccagta acgccgttcg agctgcctcc agcccgacgt ctcgatcccc
    11881 gaactgccca ttttgaccag aaccgccccg gccagatcgg tgcgagccat gtcactaccc
    11941 tgttcgcggt aacggcgcac cacggccggg tgcggatgcc cgaaacggtt gcggtacccg
    12001 accgaaaaga cgaccagccg cggtgccacc gcacgcacga attccggcgt ggaggaagtg
    12061 cggctgccat gatggggtgc gatcagcacg tcggcggaca aacgccccgg ggaatcgcgc
    12121 agcagcgcga tttcgctgcg tcgttcgatg tctgccggta gcagcacggc tccgtaaggg
    12181 gaaacgatcc tgagcacgca accacgatcg ttgtccttga gatcgaattc attataactt
    12241 tcggcatcgg gatgcagcat ttcgaaagcc accccgtccc actcccagcc ctctccggcg
    12301 ctgcaacgcc ggccgtgtgc tgctgcttcg ctgatcggat gaacgtcgga cagcggggag
    12361 gacaaccact gcaccggcat cgcctgcaat accgccagtg cgccaccgct gtggtccagg
    12421 tcgtcgtgac tgacaatgat gccgtccaga ccgcgtacgc cgcttgcccg cagataaggc
    12481 accacgaggc gttcaccggc gctcacctga tctgaaaatg ccggcccggt gtcgtagagc
    12541 agcgcgtgcc catgggtgcg caccaccacc gccaacccct ggccgacatc caatacggtc
    12601 agccacagct cgccgggcga gggccgcggc ggcaaccaca ggaacagcgg cagcatgagt
    12661 gcggcaccca cccaacgcgc gggaaaaccc ctgggtagca gtatccacag cgcgcctatc
    12721 atcgccagcg gcaccgccca ggaaggcggt gcatgttgct gccacgccgc atcgggcaca
    12781 cggcccagcg tatccagcac aatcatgcaa agcgccatga ccgcgtgcgc ggcgtgcgcg
    12841 agccaatcga gcggcagaac ggcggcaagc agcgttagcg gcaccaccac cagactcacc
    12901 aacggaatcg ctaccgcgtt cgcgatcggc gacaccagcg atacctgctg aaacatcgcc
    12961 agcaacagcg gcaccagcgc cagcgtgacc gcccactggg tgcgcgccca cgtacccagc
    13021 caggaagacc ggccgatgcg accgccgccg gcgagcatga tcaccgcgac cgcgccgaaa
    13081 gacagccaga acccggcgga aattaccgcc atgggatcga ggacgatcac cagcgccgcc
    13141 gccgctgcca gcaccgcaag cggccgcgtc atccatccca gccagagcgc cactgccaca
    13201 atcgccagca tgaaaacggt gcgttgcgcc ggcaccgcga atcccgacag cgctgcgtag
    13261 gcaagcgcgg ccgcaaggcc ggcggcggcg gcggcgcgcg ccgcgggcag ccgcagagtc
    13321 agcgtttcgg actggcgcca caaacgcaac accagcaggt acgccaggct cgcgaccatg
    13381 gtgatgtgca gcccggaaat gctcatcaga tgattgacgc cggtgcgcgt gaaaacccgc
    13441 cactgcgacg gcggaatggc acgctggtca cccaccgcaa gggcaacgat cactccggcg
    13501 taaggccggc catcgagcgc acgcagcagt ctggcgcgca ccgcttcgcg cgcgcgctca
    13561 agaacatatg cgggacggtt cacccgcgcc gccatcaggg ccgccggcgc atcggctctc
    13621 acgtatccga ccgcgcgtat gccacgctcc agcatccatg cctcgaagtc gaatccgtcc
    13681 ggattggcgt tgccatgagg gcgtcgcagg cgcgcgctca ggcgccagcg ttgccccggg
    13741 tgcaggtcgg gagcctgcgc ggtgtcgccc gactgatacc aggtgaggga aatccgtggc
    13801 ggcacttcgg caccgggtgt gtgcgccctt tcaacgtcga aggcgaagcg cacgccgcgc
    13861 tcggcaggct gggtcatttc agcgatgact cccgacaact cgatatcgcg cccttcccac
    13921 tgttccggca gcgcgtgcgc aagactccag tggccgatcg cggcggacca gaagaacccg
    13981 gcgatcgctg aagcagccaa agcggcggcg cgtgctgcca accctgcggc tgcgcctttt
    14041 ctgcgcgccg caagcagcca cagcgccgcc aaaggaacca gtaccagcgc accggtcaaa
    14101 tcaggaagca ggggctgttg ctgcagccac caggcgcccg cagtcagggc ggcaacgtta
    14161 acgatcatgc agagcggttc cggtacaatc catcacctcc atgccacgaa agtttttccg
    14221 caagtacctg ccgagccacg agagcgtccg ccagaaccgg cacattcgtt ttttcggcac
    14281 ggcgttgcat caccccaacc tgtggcacct gaaccgcaag tcggtggcgg gcggcaccgc
    14341 cgtcggcctg ttttgcggac tggtgccggg gccgctgcag atgctttcgg cggcgctgct
    14401 cgcgatcctg ttccgcgtga atctgccggt gtccgtgatc ctcacctggt acaccaaccc
    14461 gttcaccatc gttccgcttt attacatcgc ctacaagctc ggcatgttcg tcaccggcgg
    14521 caacggccat gtccttccgg gcggcgaact cgatctgctt gcgctgccga tcatggaatg
    14581 gataccggcg ctgtaccact ggctggctgc gatggggaag ccgttcgcgc tcggcctgtt
    14641 cctgctggcc ctgattctcg cagtcgccgg ctatctcctg gtgattgctg cgtggcgggt
    14701 gcacgttacg ctggcgtgga acaaacgccg gcgcatgcgt atcttcgcag cgagaaaatg
    14761 acaggcgcgg cgccttctgt cacgtgccgg gactggcgtg tatattcgaa ccgggcacgt
    14821 ttcctcccag tgaggtccga ctcggcagcc aggcaaccat ctgttgaatc atgacgccat
    14881 cctattggaa tgatgccacc cgcgatctgg cgagccgcga ttccgttctg aaaatactga
    14941 tccgaaacct tgaagggccg cagttgcaga gccgcagcga cgccttcacg acgctagcac
    15001 gctccatcat cggccagcaa atatcggtgc aagcggcgga aagcgtgtgg cagaaactgc
    15061 tggaatcggt cggatcgctc ggaccggcga ccatcgtacg catggacccc tcgaaactgc
    15121 gtgcctgcgg attgtccagc aagaagtcca cttacctgat cgacctggcc gagaattttc
    15181 tcgacggctc gctggagctc gagcgttgga gcgaccacga cgacgagcaa ctaatcgccg
    15241 agctcaccag ggtcaaggga atcgggcgct ggaccgcgga gatgttcctc atctttttca
    15301 tgatgcgacc gaacatcctg ccggtggacg acatcggcct gcagcgcgcc atgggtctgc
    15361 attacaacaa cggcaagccg ctctcgaagc tgaagatgcg ccagatcgcc accgagtggc
    15421 aaccgtggcg cagcgtggcc acctggtatc tgtggcgctc actcgatccg ataccggtcg
    15481 agtactagcc ggtgaggcga taggcgtgag gcgttaggcg tgaggcggga ggggtgatgg
    15541 gaaaagtact ataaataaaa cctctcgccc ggacttccag tccccaaagc gtatcgtcag
    15601 tcctccctgc tcttccgcct ctcgcctcac ccctcacgcc tcacggagtt tcccgtcctc
    15661 cagccgcagc gtccgctgca tcttcgccgc aatcgtcgga tcgtgagtga ccaccacgaa
    15721 actggtggcg agatcgcggt tcagttcgag catcaggtcg agcatgcctt ccgcggtgtg
    15781 gcggtcgagg ttgccggtgg gttcgtcggc gagcacgcag gccggttcgg tgaccagcgc
    15841 tcgcgccagc gccgcgcgct gccgttcgcc gccggagagc tctcccggcg tatgcgtcat
    15901 gcgcgcgctc aggccgaccc gctgcagaat cccggctgcg cgtttgccgg cctccttcga
    15961 gtcaaggcgc cggatcagca gcggcatcgc cacgttctcc agcgcggtga actccggcaa
    16021 caggtggtgg aactggtaaa cgaagccgag cgcgcggttg cgtaacctcc ccttttcctt
    16081 ctcgttcaac gagtccagca ggtggcggag cacggtgacc tctcccgcgc tcgcagcgtc
    16141 gagcccgccg agcaggtgta gcagtgtgct tttgcccgag cccgacgcgc cgacgatcgc
    16201 caccgtttcg ccgcgcgcga cgtcgatgtc gatgcccagc aacaccggga ccgcgtactc
    16261 gccctggtga aaggttttct gcagattgcg gcaggagaga acaacctcac tcatagcgca
    16321 gggcttcggc cggattcacc cgcgatgccc gcaggctggg atacatggta gccagcaggc
    16381 tcagcccgaa cgaggtcagg ccgatcacga ccacgtcctg cagatgtaga tcggagggca
    16441 ggtcgctgat gtagtacacg tccttggcga ggaactggac gcccaggaca tgctcgatcg
    16501 ccgggatcac gacttcgatg ttcgccgcca gcagcacacc gcagatcacc ccggcgatca
    16561 tgccgactgc gccgatcaac gcgccctgga taatgaagat ccgcatgatc tcccccggcg
    16621 ccgctcccag cgtgcgcaga atggcgatgt cggcgcgctt gtcggtgacc gccatcacca
    16681 gcgtggaaac gatgttgaac gccgccaccg caacgatgag caccaggatg atgaacatca
    16741 cccgcttctc gatctgcacg gcacgaaaga aattggcgtg gctgcgcgtc caatccgtgg
    16801 catagacgtc gctgctcagg gtgcgcatca gatcgcgtgc cacccgcggg gccttgaaca
    16861 ggtcgtggag cttgagccgc acccccgaca ccttgtcttc cagacgataa agcttctgcg
    16921 cgtcttcgag gtgcatgaac gcaaggccag cgtcgtattc ggccatgtcc acttcgaaaa
    16981 tgccgaccac ttcgaattgc ttgagccgcg gcaggatgcc tgcgggtgtt acctgtccct
    17041 gcggcgcgat cagcacgatc ttgtcgccga cgcgggcgcc tacgctcatc gccagatcga
    17101 cgccgagaac gatgccgaag cgccccggcc tcaggtcgtc caggctgccc gatcgcatgt
    17161 gctggcctac gtcggcgact tttgcttcgg cggcgggcag aaccccgcgc accagcgtgc
    17221 cctgcacgct cccgtggtgg gaaatcagcc cctgcgccgt gatgtacggc gcagccgcga
    17281 tcacttccga atggctttgc gcttgcgcgg cgattttccg ccagtcggac agcttgccgt
    17341 ccagtcccat gatctgcacg tgcgaagcaa ccccaaggat gcgcgtgcgc agttccttct
    17401 ggaatccgtt catcaccgag agcacgacga tcaacgccgc gacgcccagc gcgacgccca
    17461 gcatcgaggt gatggaaatg aaggagatga aatgattgcg ccgcttggcg cgcgtatagc
    17521 gcagcccgac aaacagctcg tacggcgtca gcatgaaagc ttcaaataag cgcggtggcg
    17581 cgatcggatg gcgaaacaaa gtctgccaca ttccggcgcg gatgactaag cagcctgcac
    17641 gccgccaccg gcggcggctc cgatgtcgcg tgctaaactc ggcacagttc cagtcctctc
    17701 tcccgtgcac accgtcaccc tgtttgttcc ttccctgctc tggcccgaaa cgggcgatcc
    17761 ggacgcctat cctggaccga gactgcccgc gctggaaacg ctcttgagcc gcggcagcgc
    17821 ggccgaaagt gcatgcgaaa acgagcccgc gtggctgtgc gaacgcttcg gagtacagaa
    17881 acagatcgac tggccggtcg cgccgctcgc ttttgccggt gatggcggag aagccggtgc
    17941 cgactactgg ttgtgcgccg acccggtgca tctgcgcctg cacaacgacg ggctgatcct
    18001 gctggccccg gagtccctgg agttggccga gggcgaatcg cgcgaattga ctggggcact
    18061 caatcggcag ttcgagaccg agggttttgt ctttcacgca ccgcatcctc gacgctggta
    18121 cttgaaattg ccgcaagcgc cgaagatccg taccgtcacg ctcaagcagg cgatcggccg
    18181 cgacgtcaac cgtctgctgc cagaaggcga ggagcgcctg cgttggcacc ggattttcaa
    18241 tgaagtgcag atgctgctgc acgccgagcc agtcaacgca gcgcgcgaag atcgcggtgt
    18301 ggcggtggcg aacagtctgt ggttctgggg cgggggcacc ctgcccgcat cgagaagctc
    18361 cttcgatgcg atgcagagcg acgatcccct tgccgtgggt ctggcaaggc tggcgggcat
    18421 catctccgct ccgcttcccg tcgacggcgg gttcgccgcc gggaacaaca cgcttgtcga
    18481 tcttcgggac gcggagcgcg aattcatgcg cggcaacgca gccggctggc ggcatgcgct
    18541 cgaaaccctg gaaacgcggt ggttcgcgcc gcttttcgac tgcttgcgag cgggcaggat
    18601 tggcaaggcg gtaatcgcga ccgtcgccga tggccgcaga tacgagtggt cggtcacgcg
    18661 cggcgttcgc tggcagattt ggaagaggcc acgaccgttg gctcaccatg ccggcaatgt
    18721 tctgcaccgc cacgacccgg cacggcttgc atgatcactc ggcgcatcgt cacccgcagc
    18781 gtaccgcagc cggctttcga ttcgctgtgt gcgaacggcg tgcacccggt gttggcgcgc
    18841 gcctatgccg cgcggggcgt aagcacgccg cggcagctcg atacctcgtt caatgggctg
    18901 gctccggccg agcgcctgaa aaactgcgtt gcgatggccc gcctgctcgc cgatgcaatc
    18961 gccgccaggc agcgcctgtt gatcgtcgcc gattacgacg ccgacggcgc cacggcctgc
    19021 gccctgggga tgatcggtct gcagagcttc ggagcgcagg tggactacct ggtacccaac
    19081 cgcttcgagt acggctatgg cctcacgccg gaaatcgttg ccctggcagc agcgcgccgg
    19141 cccgatttcc tgatcacggt cgacaacggc atcgccagca tcgacggcgt cgaggccgca
    19201 cgcaagctcg gtatccgggt gctcatcacc gatcaccatc tgcccaccgc acaactgccc
    19261 gccgcggcct gcatcgtcaa cccgaatcag ccgggctgcg atttcccgag caggaacctg
    19321 gcaggggtgg gcgtgatgtt ctacgtgttg ctggcgctgc gcgccgagtt gcgtgcgcgc
    19381 ggctggttcg gcggccgcga cgaacccaac ctcgcagacc tgctggacct ggttgcgctc
    19441 ggcaccgtcg ccgacgtggt gcggctcgac gacaacaacc gcatcctggt ggaccagggc
    19501 ctgaagcgca tccgggccgc ccgcgcccgc cctggaatca ccgcattgtt gcgggtggct
    19561 ggccgcgatc cggcacgcgt cgtggcttac gatctcggct tcgtcctcgg accgcggctc
    19621 aatgccgccg gccgactcac cgacatgagc gtcggcatcg agtgcctgct ggcacgcgat
    19681 agcgaccgcg ccgccgaact ggcggcacag ctcgacgcgc tcaaccgcga gcgccgcgac
    19741 atcgaagccg ggatgcagga gaccgcgctg gcgctgatag agcgaatcgc gcccgcggat
    19801 gcctacagcc tgagcctgta tgacgagact tggcatcagg gcgtggtcgg gatcctcgcc
    19861 gcgcgcctgc gtgaacgctt caatcggccg gtgatcgcgt tcgcgcccgg tggcaacggc
    19921 gaggcgaaag gctcgggccg ctcgataccg ggtctgcacc tgcgcgacgc gctcgatctg
    19981 gtatcgaagc gccaccccgg cctgatcctg cgcttcggcg gccacgccac cgcggcgggc
    20041 cttacggtgc gtagcgccga catggccgct ttcgagaatg cgttcgaaca gaccgtacgc
    20101 gggctgctga cgcctgcaga cctggagcac gtgatcgaaa ccgacggcgg actggaacct
    20161 gacgacgtca gcttgccgtt cgcggagttg ctcgccgcgc aggtctgggg ccagggattc
    20221 ccgcccccgt cattcttcga cgaattcgaa gtgacggaac agaaagtcgt cggcagccgg
    20281 cacctgaagc tgcgtctgcg ccgcaggctg gccgaacagg cattcaacgc catcctgttc
    20341 ggccgcagcg caccccttcc ctcgcgcgtg tccgcggttt accgtgtgca tgtcaacgag
    20401 ttcaacggca tgcgctcggt gcaactggtg ttggatcact ggcaggcggg ctaggcttag
    20461 ggagacaggc agtggacttc tactggctgc agggcacgga actggagcgg ttgcccgcct
    20521 ttgccatcag cctggccatc ggcctgctga tcgggctgga acgcgaacgc cacccggata
    20581 ccaacgccgg gttgcgcacc ttcgccctca ccgctttgtt cgcggcagtg gccgctctgc
    20641 tcagcgacga gaccggctcg ccggcaattc tgatcatcgg gctggtgatc gtcgccgcaa
    20701 tgattgtcac cgccaacgcc cgcgaccggg aacagcacga tcccggcacc accacggtca
    20761 tcgccctgtt gctgtgctac gggctgtccg tcatgacgtg gttcgagtac cagacgctgg
    20821 cggtgatgct ggcgatcgtc accaccagcc tgctgtactt caaggccgaa ttgcgcggca
    20881 tcgccgagag gctcgagcga cgcgatctga tttcgatcct gcagttcgcg gtgctgatgt
    20941 tcattgtgct gcccattctg ccgaatcggg atttcggccc gcacggcgca ctcaatccct
    21001 accagatctg gaccatggtg gtgctcattt ccggggtcag tctcgccggt tacgttgccg
    21061 tgcgcctggt cggcctgcgt tacggcgcac ctttgcttgg attgctgggc gggctggtgt
    21121 ccagcaccgc caccacgctg atttacgcgc gccatgcccg ggcgcaggaa agactcagcg
    21181 gcttagctgt gctggtcatc gtgctggcca acctggtgct gctggtgcgg ctggcggtgc
    21241 tcactgcttt cgtcgcgccg ggactgctgg gcactttact gccgctcctc ggcggcggtc
    21301 tgctgctcgg cctggcgagc ttgggctatc tatatgcacg tcaggggcgg gacaacacgg
    21361 cacctatccc ggatatcagg aatcctgccg agctgcgcac cgcgctgacc tttggcgcaa
    21421 tctacgcact ggtgctgctg ctcgccgcct ggctttcggg cctggctgga gctgccgggc
    21481 tgtatgcggt agcactgatt gccggactca ccgacgtgga tgcaatcacg ctggcgagcc
    21541 tgcggctctt cagcgtcggc actgtcgagg ctcacgaggt agcgatcgcc gtggtgctgg
    21601 cgatcggggc aaacattgta ttcaagctcg gcatggtact gattgtgggc ggccagtcgc
    21661 tgtttgcgcg ttgtgcggga tcaatggccg cggtcgcggc cggtatgatc gtcgcgttgt
    21721 tgttcacatg acgaactgcc cggtgcgcgg catcggttaa aatgacccgc tccttagctt
    21781 cgtctcaacc cccggatcgg aatccaaaat ggaagcagaa cgccttaacg ctatcgaaaa
    21841 acaactcgac gacctcgata cccgggccaa agaactgcgg aggtatcttt gacttcgatg
    21901 gcaaacgcaa ccgcctgatc gaagtcgtca agctgaccga agaccccgcg ttctggaacg
    21961 acgccaagcg cgcccaggac ctcgggcgcg agcgcaagaa cctggagtcg ctggtcacga
    22021 ccctggaaaa gctcgaccag gggctgaagg atgcgcgcga actgttccag atggcgcggt
    22081 cggaaaacga tgacgccacc ctgaacgccg ttgccgacga cgttgccggc ttggagagga
    22141 tcgtggcgga catggagttc cgccgcatgt tctccaaccc catggatccg aacaactgtt
    22201 tcctggatat ccagtccggg tcgggcggca ccgaagcgca ggactgggcg tcaatgctgg
    22261 aacgcatgta cctcaagttc tgcgaacgcc gcggcttcaa ggtcgaggtg ctggaggagt
    22321 cctcgggaga agtggccggg atcaagagcg ccacgctcaa ggtcaacggc gactacgcct
    22381 acggcacgct gcgcaccgag agcggcgtgc accgcctggt gcgcaagtcc ccgttcgact
    22441 ccaatgcgcg ccgccatacc tccttcgcca gcgttttcgt ctatccggaa gtagacgaca
    22501 cgatcgaaat cgcgatcaat ccagcggacc tacgcgtcga cacctttcgc gccagcggcg
    22561 ccggtggcca gcacatcaac aagaccgact cggcgatccg catcacccat gtgcccacag
    22621 gtatagtcgt gcagtgccag aacgaccgct cccagcatcg caaccgcgcc gaggcgatgg
    22681 caatgctgaa ggcgcgcctg tatgaactcg agctgcgcaa gcgcaacgag gaaaagcagg
    22741 cgatggagga ttccaagacc gatatcgcct ggggacacca gatccgttct tacgtgctcg
    22801 accagtcgcg catcaaggat ctgcgtacca acgtcgagat cggtaacacc caggcggtgc
    22861 tcgatggtgc cctcgatccg ttcatcgagg ccagcctgaa acaaggcgtt taacaccgtg
    22921 actatcgcga cgatcgtgac tggcgtgaat ggcaacacgc gattcattgg ccccgccctg
    22981 catctgcgac agtcaagaca attgcgactg tcacgtcatt aaaggccaaa agacatggaa
    23041 gaaaaaaacc aactgccgat tgttgacacc aaccagatcg tcgaggagcg ccgtgccaag
    23101 ctgcgcgaat tgcgtgcgca gggcaatgcc tttcccaacg atttccgccg ccgccacctc
    23161 gccgccgacc tgcatcgcgc ctacggcgcc acgccgaacg aagaactgga gccgctgaag
    23221 gccgaagcga cggttgccgg gcgaatgatg ctcaagcgcg tgatggggaa ggcaagtttc
    23281 gccaccatcc aggacatgtc cgggcgcatc cagatctatg tcaccaatga cgtcaccggc
    23341 gaaccggtac acaccgcatt caagcactgg gacctgggcg acatcgtcgg cgcgaccgga
    23401 acgctgttca agaccaggac cggcgaactc accgtgaaag ccgccggcgt gcacctgcta
    23461 gccaagtcgt tgcggccttt gccggagaag ttccacggtc tggccgacca ggaagccaaa
    23521 taccgtcagc gatatctcga tctgatcacg aatcccgaag cgcaacgcgt gttcaaaacg
    23581 cgctcgcgga tgattcaggc aatgcgcgaa ttcttcgtcg cacgcggtta tctcgaagtc
    23641 gaaacgccga tgatgcagcc catccccggc ggtgcggcgg cgcggccgtt cgtcacgcat
    23701 cacaacgcgc tcgacatgga cctctacctg cgcatcgcac ccgagcttta cctcaagcgg
    23761 ctggtggtgg gcggaatcga gaaggttttc gagatcaacc gcaatttccg caacgaaggc
    23821 atctcgaccc ggcataaccc ggagttcacc atgctggagt tctacgaggc ctaccaggac
    23881 tacaactatc tgatgggcct caccgaagaa ctgttgcgcc aggtcgcgcg ggaggtgctc
    23941 ggaacaacca cgttcagcta tcagggtgag accatagatc tgggcaaacg cttcgatcgg
    24001 ctgaccatgg ccgaggccat cgccaagtac aacccggaat acccgatggc ggaacttggc
    24061 aagaccgcgt tcctgcgtac agcgctcgat gctttcaaag gcgaggtttt ctctactgac
    24121 ggcttgggat tgctgcaact caagctgttc gagcagacca cggaggacaa gctcatccag
    24181 ccgaccttca tcgtcgccca tccgaccgac gtatcgccct tggcccgccg caacgacgct
    24241 aatccggctg tcaccgaccg tttcgagctg ttcgtcaccg gccgcgagat ggcgaacgga
    24301 ttctcggaac tgaacgaccc ggaagatcag gcagagcggt tctcggagca ggccagggcc
    24361 aaggaggccg gcgacgagga ggcgatgtac tacgacgccg attacgtgcg cgcactcgag
    24421 tacggtctgc cgcccacggc gggagaaggc attggcatcg atcgcctggt gatgcttttc
    24481 accgatagtc cctcgat
//
LOCUS       DASQOP010000169         4733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1478027, whole genome shotgun sequence.
ACCESSION   DASQOP010000169 DASQOP010000000
VERSION     DASQOP010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4733
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478027"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="VE030_06870"
     CDS             complement(<1..821)
                     /locus_tag="VE030_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZF23169.1"
                     /translation="MAQSTKHSRLLILGSGPAGYTAAIYAARANLKPVLVTGLAQGGQ
                     LMTTTDVDNWPADVMGVQGPELMERFHKHAERFDTEIIFDHIHSVKLGHRPFQLTGDQ
                     ALYTCDALIIATGASAKYLGLPSEQAYMGKGVSACATCDGFFYKGQDVAVVGGGNTAV
                     EEALYLSNIAKNVTVVHRRDKFRAEGMLIDRLMEKTRSGNVKIEWNHTLDEVLGDKKG
                     VHGVRIRPTHGDKARELPLHGVFIAIGHTPNTQIFRGQVEMVNGYIVTKGGSEGNA"
     gene            961..3258
                     /locus_tag="VE030_06875"
     CDS             961..3258
                     /locus_tag="VE030_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HZF23170.1"
                     /translation="MRTLSASRSAAHKPPRKPLPPKIAALLRESWWLLLVGAALYLVL
                     ILFTYSKSDPGWSHQATGSLVANAGGKAGAWMADILLYVFGLSAWWGVFFLLAAVVWG
                     YRRLDAPEMDRRSLVVACSGFLLLVTGSSALESLRLYTLHVSLPHGPGGVLGDLVSGL
                     FSKAFGFTGSTLLLLLLCGVGLSLCTGMSWVAFIEKLGAAIEGAYTFLVERWQTRQDK
                     KAGEQAVVEREELVEVERKRFEEHDPIRIESPRTVIPKSDRVIKEKQKPLFEDLPDSP
                     LPPLHLLDEPPADLELLSAETLEYTSRLIEKKLGDFDIEVNVVAAQPGPVITRYEIEP
                     AVGVKGSQIINLVKDLARALSVVSVRVVETIPGKSCMGLEIPNPKRQVVRLSEILSSK
                     VYAEMGSALTLALGKDIAGNPVVADLGRMPHLLVAGTTGSGKSVALNAMILSLVYKAP
                     AKDVRLILVDPKMLELSVYEGIPHLLAPVVTDMKQAAHALNWCVGEMDRRYRLMSTLG
                     VRNLSGFNQKIKEAEKAGRPLTNPFTITPETPEPLTELPLIVVVIDELADLMMVVGKK
                     VEELIARLAQKARAAGVHLILATQRPSVDVITGLIKANIPTRVAFQVSSKVDSRTILD
                     QMGAEQLLGQGDMLYLPPGTGYPHRVHGAFVADQEVHKVVEYLKARGQPQYIDDVLEG
                     GETDAEEGAAAGEAEGEADPLYDQAVEIVLKSRRASISLVQRHLRIGYNRAARLIEQM
                     ERAGMVSTMQPNGNREVLVPSRTEA"
     gene            3323..3910
                     /locus_tag="VE030_06880"
     CDS             3323..3910
                     /locus_tag="VE030_06880"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HZF23171.1"
                     /translation="MRKLLILIAFQALAPAAIADVYKWVDRDGGVHYGDTPPANTGSR
                     PVDLRFAGSATTTEAISCCAAKEVPPEPAGQPTYIIFPAEPPARSPASRGMDFGVFIM
                     LRHGMSEGELLQRAGPPDFQSADGYAVRPIVVTRRGRPIGQLVDWRGQGALSYGVSNQ
                     IVKTFYYYPTISNPFTTVLTLTGGRITDLQRTRQF"
     gene            3910..4530
                     /gene="lolA"
                     /locus_tag="VE030_06885"
     CDS             3910..4530
                     /gene="lolA"
                     /locus_tag="VE030_06885"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HZF23172.1"
                     /translation="MRRAFPISALFFSGLLAFPAHAGPIEKLHAFIEQTRSARATFTQ
                     TVADSSGQITQQASGTVQFQRPGRFRWTYSKPYEQLIVGDGEKLWIYDKDLNQVTQRK
                     LDKALGSSPAALLAGADDVEKYFSLNALGKKGKLDWLEVKPYDEDSLFDKVRMGFNRN
                     MLEVMELYDHFGQKTTIKLSRLERNPVTRADTYTFTIPDGADVVTE"
BASE COUNT          920 a         1435 c         1504 g          874 t
ORIGIN      
        1 gcgttgccct cgctgccacc cttggtgacg atgtagccgt tcaccatctc gacctggccc
       61 cggaagatct gcgtgttcgg ggtgtggccg atggcgatga ataccccgtg caacggcaac
      121 tccctggctt tgtcgccgtg cgttggcctg atgcgcacgc cgtggacacc tttcttgtcg
      181 ccgagcacct cgtccagcgt atggttccat tcgatcttca cgttgccgga cctggtcttt
      241 tccatcaggc ggtcgatcag catcccctcg gcgcggaatt tgtcacggcg gtgcaccacc
      301 gtcacgttct tggcgatgtt ggacaggtac agcgcttcct cgacggcggt gttgccgccg
      361 ccgaccaccg ctacgtcctg tcccttgtag aaaaagccgt cgcaggttgc gcacgccgat
      421 acgcccttgc ccatgtaagc ttgctccgag ggcagaccga ggtacttcgc cgaagcaccg
      481 gttgcgatga tcagcgcgtc acaggtatag agggcctgat cgccggtcaa ctggaacggc
      541 cgatggccca acttcacaga atgaatgtgg tcgaagatga tctcggtgtc gaagcgttcc
      601 gcgtgcttat ggaagcgttc catgagttcc ggtccctgca cgcccatgac gtcggcgggc
      661 cagttgtcca cgtcggtggt ggtcatgagt tgcccgccct gagcgaggcc ggtgaccagg
      721 accggcttga ggttggcgcg ggccgcatag atcgcggcgg tgtagccggc cggaccggaa
      781 cccagaatga gcaaacggga atgctttgtt gattgagcca tggcagtgtt aaatcggggt
      841 aaaggcaaaa atagcaagac tgtagcagcc gcgcgaagtt cgtgtccacg cgtgcaaaat
      901 gggctgcgtt gagaccgcgg ccctgggata taattctttg aaactgaaaa ggtaatttgc
      961 ttgcgaacac tttcggcgtc cagatccgca gcccataagc cgccgcgcaa gccgctgcca
     1021 ccgaagatcg ccgccctgtt gcgggaatcc tggtggctgc ttctggtcgg cgcggcgctc
     1081 tatctggttc tcatcctctt cacctacagc aagtccgatc cgggctggtc gcaccaggcc
     1141 accggctcgc tggttgccaa cgccggaggc aaggccggcg cgtggatggc cgacatcctg
     1201 ctctatgtat tcgggctgtc ggcgtggtgg ggggtgttct tcctgctcgc tgccgtggtc
     1261 tggggctatc gccgcctcga cgcaccggaa atggaccgcc gctctctggt cgtcgcgtgc
     1321 agcggtttcc tgctcctggt caccggcagc agcgcgctcg agtcgctgcg cctgtacacg
     1381 ctgcacgtgt cgctgccgca cgggccggga ggcgtgctcg gcgacctggt gagcggtttg
     1441 ttctcgaagg cattcggctt taccggcagt accctgctgc tgctgttgct gtgcggggtg
     1501 gggttgtctc tctgcaccgg catgtcctgg gtcgcgttca tcgagaagct cggcgcggcg
     1561 atcgagggcg cctacacctt cctggtggag agatggcaga cccgccagga caaaaaggcc
     1621 ggcgagcagg ccgtggtcga gcgcgaagag ctggtggaag tcgagcgcaa gcgcttcgaa
     1681 gaacacgacc cgatccgcat cgagtctccc cggaccgtga tccccaagtc cgaccgcgtc
     1741 atcaaggaaa agcagaagcc gctgttcgaa gacctgccgg attccccgct gccccctttg
     1801 cacctgctcg atgagccgcc tgccgacctg gaattgctgt ctgccgagac gctggagtac
     1861 acctccaggc tgatcgagaa gaagctcggc gacttcgaca tcgaggtgaa cgtggtggcc
     1921 gcgcaaccgg gcccggtgat cacgcgctac gaaatcgagc cggcggtggg cgtgaagggc
     1981 agccaaatca tcaatttggt gaaggacctg gcgcgcgcgt tgtcggtggt gagcgtacgt
     2041 gtagtcgaaa ctattcccgg caaatcatgc atgggactgg agatccccaa ccccaaacgg
     2101 caggtggtgc ggctctcgga gatcctcagt tcgaaggtgt acgccgagat gggttcggcg
     2161 ctcacgctgg cactgggcaa ggacatcgcc ggcaatccgg tcgtcgcaga cctcggcaga
     2221 atgccgcatc tgctcgttgc cggcaccacc gggtcgggca agtcggtggc gctcaacgcc
     2281 atgatccttt ctctggtgta caaggcgccg gcgaaagatg tacggctgat cctggtggac
     2341 ccgaagatgc tcgaactgtc ggtgtacgaa ggcattccgc atctgctggc gccggtagtg
     2401 accgacatga agcaggccgc gcacgcgctc aactggtgcg tcggtgaaat ggaccgccgc
     2461 tacaggctga tgtccacgct gggcgtgcgc aatctgtctg gattcaacca gaagatcaag
     2521 gaagcggaaa aggccggcag gccgctgacc aatccgttca cgatcacgcc ggaaactccc
     2581 gaaccgctaa cggagctgcc cctgatcgtg gtggtcatcg acgagctcgc cgacctgatg
     2641 atggtcgtag gcaagaaagt cgaggagctg attgcgcgcc tggcgcagaa ggcccgcgca
     2701 gccggcgtac acctcattct cgcgacccag cggccgtcgg tggacgtcat caccggatta
     2761 atcaaggcaa atattccgac ccgggtcgcg ttccaggttt cgagcaaggt cgactcgcgc
     2821 actatcctcg accagatggg cgcggaacag ctgctcggac agggcgacat gctatatctg
     2881 ccgccgggca ccggctatcc acaccgtgtg cacggcgcgt tcgtcgccga ccaggaagtg
     2941 cacaaagtgg tggagtacct gaaagcgcgc ggtcaaccgc agtacatcga cgacgttctg
     3001 gaaggtggcg agaccgacgc ggaagaaggc gctgcggcgg gcgaggcgga aggcgaagcc
     3061 gatccgctct acgaccaggc cgtggaaatt gtgctcaagt cgcggcgggc gtccatctca
     3121 ctggtgcagc gacatttgcg catcggctat aaccgggcag cgcgcctgat cgagcaaatg
     3181 gagcgtgccg gaatggtttc caccatgcag cccaacggca accgtgaagt gctggtgccg
     3241 tcgagaacgg aagcgtgagc ctgcccccga atgctgctat cggggggcga gaggcgagag
     3301 gcgagaggcg aaggaggagg tcatgcgaaa acttctgata ctgattgcat tccaggcgct
     3361 ggcgcccgcc gcgattgccg acgtatacaa atgggtggac cgcgacggcg gcgtgcacta
     3421 cggcgatacg ccgcccgcaa acaccgggtc ccggcccgtg gacttgcgtt ttgccggcag
     3481 cgccacgacg accgaagcga tttcctgctg cgctgcgaag gaggttccgc cagagccggc
     3541 agggcagcct acgtacatta tcttccccgc cgaacccccg gcacgcagcc cggcgtcgcg
     3601 cgggatggac ttcggcgtat tcatcatgct gcgccatggt atgagcgaag gtgaactgct
     3661 gcagcgcgcc ggtccgcccg atttccagag cgcggatggg tacgcggttc ggccgatcgt
     3721 ggtaacgcgg cgcggacggc cgatcggcca gttggtcgac tggcgcggcc agggggcgct
     3781 ctcttacggc gtcagcaacc agatcgtcaa gaccttctac tactacccga cgatctccaa
     3841 cccgttcact acagtgctta ccttgaccgg agggcggatc accgacctgc agaggacgag
     3901 gcaattctga tgaggcgcgc gttccccatc agcgcgcttt ttttcagcgg cctgctcgca
     3961 tttccggcac acgccggtcc gatcgaaaaa ctgcatgcct tcatcgaaca gacgcgctcg
     4021 gcgcgtgcaa ccttcaccca gactgtggcg gactcgagcg gccagatcac ccagcaggcg
     4081 agcggcacgg tccagtttca acgtcccggc agattccgct ggacttacag caagccttac
     4141 gagcaactga tcgtcggcga tggcgagaag ctgtggatct acgacaagga cctgaaccag
     4201 gtcacccagc gcaaactcga caaggccctc ggcagcagcc ccgcggcgct gctcgccggc
     4261 gcggacgacg tcgagaagta tttctcgctg aacgcgctcg ggaagaaggg caaactggac
     4321 tggctggaag tcaaacccta cgacgaggac agcctgttcg acaaggtgcg aatgggcttc
     4381 aacagaaaca tgctcgaagt tatggagctt tacgatcact tcgggcagaa gaccaccatc
     4441 aaactgtcca ggctcgagcg caatcccgtc acgcgtgcag atacctacac cttcaccatc
     4501 cccgacggcg cggatgtggt gacggaatga gttcaccaca gagacacaga ggcacagaga
     4561 aaagacacga gaatggcata gtcgaatgcg gaagacgcgg atgtctaata gtgccactcg
     4621 gtttttcaca cgatcaaggt ttggccttga atttctccgt gcctctgtgt ctctgtggta
     4681 agaactttca gtgactgatc ttttcgaagc caggcagccg gcagcgccgc tcg
//
LOCUS       DASQOP010000170         4976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_472214, whole genome shotgun sequence.
ACCESSION   DASQOP010000170 DASQOP010000000
VERSION     DASQOP010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472214"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2585)
                     /gene="pepN"
                     /locus_tag="VE030_06890"
     CDS             complement(<1..2585)
                     /gene="pepN"
                     /locus_tag="VE030_06890"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HZF23173.1"
                     /translation="MLTPAPKTIHLKDYTPPAFLVSTVDLDVDIRDDDALVKATLAVS
                     RNPQAAGSSAPLELDGDELELLSVALDGRPLDSSAYTLDVKHLTIPGVPVRFTLETVA
                     RIRPKQNSKLMGLFASKDGFFSQCEAEGFRRITFFPDRPDVMARYTTTIRADRAEYPW
                     LLSNGNLVAQGDEAHFTPTFSSASGGGGGKLQRQGPRHWARWVDPFPKPSYLFAMVAA
                     RLDRLEDSFVTKSGRKVKLLVFVEPGKLDQCKFAMAALKRAMKWDEEVFGLELDLDQY
                     MIVAVGDFNMGAMENKGLNIFNTKYVLARADTATDNDFMYLDRVVAHEYFHNWTGNRV
                     TCRDWFQLSLKEGLTVFRDQEYGADTYSRAVQRIQEVRGLRGAQFPEDAGPMAHPVRP
                     ASYMEISNFYTATVYEKGAEVVRMIHTLIGAVNFRKGMDLYFQRHDGQAVRTEEFVQA
                     MQDASGLDLTQFKSWYDQAGTPLLEVRGNYDAASGRQTLTVKQSCPPTPGQEEKLPFH
                     IPLAVGLVGPDGSDVPLQLDGENRAAGTTRVLSVKRAEEEFVFLNVTRQPVPSLLRGF
                     SAPVNLRFEYSDADLTHLMAHDSDAFSRWEAGQRLALNLLLRGIASYLAGHAPAFPDS
                     FVKAFSRVLADAAQDPAFAAEALGLPSEGYVAEQMEEVVADAIHAVRVALRKHLATSL
                     KGELLATYQAFTAPGPYSPDAKSAGQRSLRNLCLSYLMELEDATVRTLCVRQFDSADN
                     MTDSMAALTALANTDCPERFEALDKFYAKWKDESLVMDKWFAVQATSRLPDTLAAVKR
                     LLLHPAFNLKNPNKVRAVIGSFCQGNHVRFHTADGSGYAFAADQVIALDPLNPQVAAR
                     LARSF"
     gene            complement(2845..3483)
                     /locus_tag="VE030_06895"
     CDS             complement(2845..3483)
                     /locus_tag="VE030_06895"
                     /inference="COORDINATES: protein motif:HMM:NF017269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HZF23174.1"
                     /translation="MDKKIAVPLLLAACAAPGYAYDYTDTAEVVSAVPIYETVSEPRQ
                     QCWTESVTYYEESGRSNGGAIIGGITGGLLGAQVGRGNGRIAAAATAAAIGALVGDRL
                     DNRYSYSSPVTRPVQRCNVVDNYRKTISGYQVTYHYNGRNATVILPYDPGPRVRIGVG
                     ISDNGGANTYIVPQSHMVLPSVTHITYIDDRNVPVWKKKPYKRPKHDRDWDD"
     gene            complement(3628..4137)
                     /locus_tag="VE030_06900"
     CDS             complement(3628..4137)
                     /locus_tag="VE030_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZF23175.1"
                     /translation="MHPLVVRSLRTLACVALAALCTRTWTAHADVFADGDHVMLQAGP
                     YVYHRNYDPNHNNEPRLVGVEWESASRWELGASYFKNSFYQPCMYIYAGRRWFLRSAD
                     NGLYFKLSGGPLYGYKEPYEDKIPVNDNGFGLAILPAIGYQYQRANAQLVILGTAGLM
                     LTFGYDFWK"
     gene            4527..>4976
                     /locus_tag="VE030_06905"
     CDS             4527..>4976
                     /locus_tag="VE030_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZF23176.1"
                     /translation="MATLGNIINYIPINRRLLLIIWPFLVIVVILVWLSSESMDILMA
                     TRAYSEGESLWSKGQKQAIFYLLRYSETQSEVDFRKYRERIAVTLGDKKARLELEKPD
                     PDYSIAWQGFVEGDNHPEDIPRMIMLYRRFRHIDFMAKVIDLWTEGDR"
BASE COUNT          984 a         1463 c         1536 g          993 t
ORIGIN      
        1 tcgaacgagc gggccagccg cgcggcgacc tgtggattga gcggatcgag cgcgatcacc
       61 tgatcggcgg caaacgcata gccggaaccg tctgcggtgt ggaagcgcac gtggttgccc
      121 tggcagaagc tgccgatcac cgcgcgcacc ttgttgggat tcttcaagtt gaacgccgga
      181 tgcaacaaaa ggcgcttcac tgcggcgagc gtatcgggca gccgggacgt ggcctgtacc
      241 gcaaaccact tgtccatgac cagcgactcg tccttccatt tcgcataaaa cttgtccagc
      301 gcctcgaatc gctcaggaca gtcggtattg gccagagcgg tcagcgccgc catcgagtcg
      361 gtcatgttgt ccgcggaatc gaactgcctg acgcacaacg ttcgcaccgt ggcatcttcc
      421 agttccatca gatacgacag gcagagattg cgcaggctgc gttgccctgc cgactttgcg
      481 tcagggctgt aggggccggg tgccgtaaag gcctggtagg tggcaagcag ctcgcccttg
      541 agcgacgtag ccaggtgttt gcgcagcgcc acgcgcacgg cgtggatcgc atcggcaacc
      601 acctcttcca tctgctcggc gacgtatccc tcagacggca ggccgagtgc ttcggcagcg
      661 aacgccgggt cctgcgccgc gtcggcgagc acgcgcgaga acgctttgac aaaggaatcg
      721 ggaaacgccg gtgcatgacc cgcgagatac gacgcgatgc cgcgcaggag caaattgagt
      781 gcgagtcgct gtcccgcttc ccagcggctg aaggcgtcgg aatcgtgcgc catcagatgg
      841 gtgagatcgg cgtcgctgta ttcgaagcgc aagtttaccg gcgccgagaa gccgcgcagt
      901 aatgaaggca ccggttgccg ggtcacgttg aggaacacga attcttcctc ggcgcgcttc
      961 acggacaaca cgcgtgtggt gccggcggcc cgattctcgc cgtccagttg caaggggaca
     1021 tcgctgccgt ccgggccgac cagtccgacc gccagcggga tgtggaacgg cagcttctct
     1081 tcctgccccg gtgtcggcgg gcaggactgc tttacggtca gcgtttggcg cccggaggct
     1141 gcgtcgtaat tccctcgcac ctccagcaac ggcgtgccgg cctggtcata ccagctcttg
     1201 aactgggtga ggtccagacc cgaggcgtcc tgcatggcct ggacgaattc ctcggttcgc
     1261 actgcctggc cgtcgtggcg ctggaagtac agatccatac ccttgcggaa atttacggct
     1321 ccaatcagcg tgtggatcat gcgcaccact tccgccccct tctcgtacac ggtggcggtg
     1381 tagaagttgc taatctccat gtacgacgcg ggccgtaccg ggtgcgccat cggaccggca
     1441 tcctccggaa actgcgcgcc gcgcagccca cgtacttcct ggatgcgctg caccgcacgc
     1501 gagtaggtat cggcgccgta ttcctggtcg cggaacacgg tgagaccctc cttgaggctt
     1561 aactggaacc agtcgcggca ggtcacccgg ttgcccgtcc agttgtggaa gtactcgtgt
     1621 gcaaccaccc ggtcgaggta catgaagtcg ttgtcggtgg cagtatcggc gcgggccagg
     1681 acgtacttgg tgttgaagat attgaggcct ttgttctcca tcgcgcccat gttgaagtca
     1741 ccgaccgcga cgatcatgta ctggtccaga tcgagctcca ggccgaacac ctcttcatcc
     1801 catttcatgg cgcgcttcag cgcggccatc gcgaacttgc actggtcgag cttgcccggc
     1861 tcgacgaaaa caagaagttt cactttgcgc ccggacttgg tgacgaaact gtcctcgagc
     1921 ctgtcgagcc ttgccgccac catcgcgaac aaatacgagg gcttcgggaa cgggtccacc
     1981 caccgtgccc aatgacgcgg gccttgccgt tgaagttttc ctccccctcc gccgcttgcg
     2041 gaggaaaagg tcggggtgaa gtgggcctca tcgccctgcg cgaccagatt gccgttggac
     2101 agcagccacg ggtactctgc gcggtctgcg cggatggtgg tggtgtagcg cgccatcacg
     2161 tccggccggt cggggaagaa ggtgatgcgg cggaaacctt ccgcttcgca ctggctgaag
     2221 aaaccgtcct tggaagcgaa cagacccatc aatttggagt tctgcttcgg tcggatacgc
     2281 gccacggttt ccagcgtgaa ccggaccggc acgccgggaa tcgtcagatg ctttacatcc
     2341 agcgtataag cgctgctgtc gagcggcctg ccgtccaacg cgaccgacaa caactccagc
     2401 tcgtcgccgt caagttcgag cggcgcgctt gaaccggcgg cctgcggatt gcgtgagaca
     2461 gcgagcgtgg cctttaccag cgcgtcgtcg tcgcggatgt ccacgtccag gtccacggtg
     2521 gaaaccagga acgccggcgg cgtgtagtcc ttcaaatgga tggtcttcgg cgcaggggta
     2581 agcattctgg tagcgtgagt cggcgcaagg tcggtcatgg aaatgcaagg atgaaggcgg
     2641 aaggatgaag gatgaagatc ggaagagcaa gaatgaagac ggaatgcaag gacgagggaa
     2701 aagaggccag tataaatgca tcatgacatc gcaaattgcc gatgtcatga cggtactcga
     2761 ccacgagatg tcaatgatca ggagcttttt tcggacgtct gccttttcac ttcatccttc
     2821 atccttccgc cttcatcctt ggtatcagtc gtcccagtcg cgatcgtgct tgggacgctt
     2881 atacggtttc tttttccaca ccggcacatt acggtcgtcg atgtaggtga tgtgcgtcac
     2941 cgaaggaagg accatgtgcg attgcggcac gatgtaggtg tttgcgccgc cgttgtcgct
     3001 aatgcctacg ccgatgcgaa cgcggggacc tggatcgtag ggcaggatca ctgtcgcgtt
     3061 gcggccattg tagtgatacg tgacctggta accgctgatc gtcttgcggt agttgtccac
     3121 cacattgcaa cgttgcaccg gtcgggtcac cggcgaggag tagctgtagc ggttatcgag
     3181 gcggtcaccg accagcgcgc caattgcagc ggcagtggcg gcagcggcaa tcctgccgtt
     3241 gcccctgcca acctgggcgc cgagcaagcc gccggtaatg ccgccgatga tggcgccgcc
     3301 gttggaacga ccggattcct cgtaataggt caccgactcg gtccagcatt gctgccgcgg
     3361 ctcgctgacc gtctcgtaaa tcgggacggc agaaaccact tcggcggtgt cggtgtagtc
     3421 gtaggcatag cccggggcgg cgcacgcagc caggagcagg ggaacagcga ttttcttgtc
     3481 cacgtcaagc ctcctttttt gcagaagata cgcctccaca aacggccgga gacggtggac
     3541 gtttaccacg tgacaattgg ttacgcagcc ggcggtgccc tccggcgagc acggccacgg
     3601 accgccttac cgacgcggat tttcccgcta cttccagaaa tcgtagccga aagtgagcat
     3661 cagtccggca gtaccgagaa taaccaactg cgcgttcgcc cgctgatact ggtaaccaat
     3721 tgcggggagg attgcgagcc cgaatccgtt gtcgttcacg gggatcttgt cttcgtacgg
     3781 ttccttgtat ccgtataggg gcccccccga gagcttgaaa tacaagccgt tgtctgccga
     3841 tctcaagaac cagcgcctgc cggcatagat gtacatgcac ggctgataga aggagttctt
     3901 gaagtaactc gccccgagtt cccagcgcga cgccgattcc cattccacgc caacgagacg
     3961 cggctcgttg ttgtggttgg ggtcatagtt gcggtgatac acataaggtc cggcctgcaa
     4021 catgacatgg tcgccatctg cgaacacatc ggcatgtgcg gtccaggtgc gcgtgcacag
     4081 agcggccaga gcgacacagg ccaaggtgcg cagcgagcgc actacaagcg ggtgcatcgc
     4141 gagccattgt tcaacgaggc ctcgggaact gcgagtaccg ggttttctgg aatattggcg
     4201 caacggattc tttccgtgtg gtgccatcgg ccgccgggtg gacgaaccgc taaacgctca
     4261 aggtccgcgc aggatatcag agccgaactt ctgcccgttg caatcgttgc gtcgggacac
     4321 ttcctcagaa aacaccgcag atgcttgatc taacgtcttt tatctactgg cattttcgtc
     4381 gtcgaggcgg tatgctttcg ggtagtgccg tggaggcact cgccgggaat ccggctcgcc
     4441 aagcaaattc tggattcagg tacgactcgc gcagacgcgc gggccggccg gtccacgttc
     4501 aaaggtccaa acgcggtctc cgggatatgg caacgctggg caacatcatc aactacatac
     4561 cgatcaacag gcggttgctt ctgatcatct ggccattcct ggtcatcgtc gtgattctgg
     4621 tatggctgtc cagcgagagc atggacatcc tgatggccac gcgggcgtat tcagaggggg
     4681 aaagcctgtg gtccaaaggg cagaagcagg cgatttttta cttgcttcgt tattcggaga
     4741 cccagtccga agtcgacttc cgaaaatacc gcgagcgaat cgccgtgacc ctgggcgaca
     4801 agaaggcccg cttggaactg gagaaaccag accccgatta ttcgattgca tggcagggat
     4861 tcgtcgaggg ggacaaccac cccgaggaca ttcccaggat gatcatgctc tatcgccgct
     4921 tccgccacat agatttcatg gcgaaggtaa ttgatctttg gaccgagggc gatcgg
//
LOCUS       DASQOP010000171         2197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2284528, whole genome shotgun sequence.
ACCESSION   DASQOP010000171 DASQOP010000000
VERSION     DASQOP010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2284528"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VE030_06910"
     CDS             <1..397
                     /locus_tag="VE030_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HZF23177.1"
                     /translation="NPGRLDGEDLRVMQSHARIGYDMLKDSSSGYLQMAAVIALGHHE
                     KYDGSGYPTGLRGEHIPLEARIVAIADVYDALTSVRPYKPAWPVEEALVYMNEHAGRH
                     FDPQLVEILARNREEVLSIGERFADRLAQ"
     gene            416..>2197
                     /locus_tag="VE030_06915"
     CDS             416..>2197
                     /locus_tag="VE030_06915"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZF23178.1"
                     /translation="MALNSILAGIRARFRGRPDTEHEQAIVRLLIAAILFLYLLPQAF
                     GGSEPNLPLFGAMICYFTLCGTVFAWIYVFPAASPARRVFGAILDVGSTSAFMYYLGE
                     YGAVLYIAYLVVTFGHGFRYGKPYLYNSLVLSVVGFALVLTFSDYWIQNRVLGIGLMV
                     GMILLSLFQGKLVTRLFDSLRREEAANQAKRRFLSTVSHEMRTPLNAIVGMNDLLRDT
                     RLDTEQAEMVKAMHEASRSMLKLIEDVLDISKIEAGKVNIEETDFDLHSLINGTAAVL
                     APQAEIRGLLFRTHVMPEVPHALRGDPYHLRQVLYNLIGNGVKFTQSGSVVLSVSSLG
                     ESEHAVRLKFSIEDTGIGIAPEAQERIFESFVQADDSTTRRYGGTGLGTTISKQLVEM
                     MGGQIGLNSTLGKGSTFWFDLPFKKQMTGQAAEASLRLNDSRIMLIGFSDVELQALEQ
                     DLATWGARTGRAAGVEAGAARLAEAQSLGHPYHLVLLREDTSIAPGRNLGRLRNSRSQ
                     KAPPTVLCTAGVSQIRSAEALAGGFVAVLEMPFKKRLLFNVIHSATAVQGSQEGVISL
                     SDYYATRDANRRRYQILVAEDNAVNQRV"
BASE COUNT          409 a          697 c          694 g          397 t
ORIGIN      
        1 gaatcccgga aggctggacg gcgaggacct gcgagtcatg cagtcccacg cgcgcatcgg
       61 ctacgacatg ctgaaggaca gctcttccgg atatttgcaa atggccgcag tgatcgcgct
      121 cggccatcac gagaagtacg acggcagcgg ctacccgacc ggcttgaggg gcgagcatat
      181 cccgcttgag gcgcggatcg tcgccatcgc ggatgtctac gatgcgctca cctcggtccg
      241 cccctataaa ccggcctggc ccgtggagga agctctcgtc tacatgaacg agcacgccgg
      301 caggcatttt gatccgcaac tcgtggaaat cctggctcga aacagagaag aagtcctttc
      361 catcggcgag cggttcgccg atcggctggc gcagtagacc ccgggacccg gccggatggc
      421 gctgaattcg atccttgcag ggattcgtgc ccgctttcgc gggcgccccg acaccgagca
      481 cgagcaggcg atcgttcggc tgttgatcgc ggcaatactg ttcctgtatc tcctgccgca
      541 ggcctttggc ggcagcgaac ccaatctgcc gctgttcggc gccatgatct gctacttcac
      601 gctatgcggc acggtattcg cgtggatcta cgtcttcccg gcggcatcgc ctgcgcgccg
      661 tgttttcggc gcaattctgg acgtcggctc gaccagcgcg ttcatgtatt acctgggcga
      721 atacggcgcg gtgctgtaca tcgcttatct ggtggtcacc ttcggccacg gtttccggta
      781 cggcaagccc tatctgtaca actcgctggt actcagcgtc gtgggtttcg ccctggtcct
      841 gacgttcagc gattactgga tacaaaaccg cgtgctcggc atcggcctga tggtgggcat
      901 gatcctgttg tcgctcttcc aaggcaagct cgtgacccgc ctgttcgatt cgctgcgtcg
      961 cgaggaagcc gcgaatcagg cgaagcggcg cttcctctcc acggtcagcc acgagatgcg
     1021 caccccgctc aacgccatcg tcgggatgaa cgacctgctg cgcgacacac gcttggacac
     1081 ggaacaggcc gaaatggtga aggcgatgca cgaggcctcg cgatcgatgc tcaagctgat
     1141 cgaggacgtg ctggatattt ccaagatcga agccggtaag gtcaacatcg aagaaacgga
     1201 tttcgatctg cacagcctga tcaacgggac cgcggcggtg ctcgcgccgc aagccgagat
     1261 ccgcgggctg ctgttcagga cccacgtgat gccggaagtg ccgcacgcgc tgcgcggcga
     1321 tccctatcat ctgcgccagg tgctctacaa cctcatcgga aacggcgtca agttcaccca
     1381 gtccggcagc gtcgtcctga gcgtttcgag cctgggcgag agcgagcatg cggtgcggct
     1441 gaaattttcc atcgaggaca cggggatagg gatcgcgccg gaagctcagg aacgcatttt
     1501 cgagagtttc gtccaggccg acgactcgac cacgcgccgc tacggcggca ccgggctcgg
     1561 caccaccatc tcgaagcagc tggtggagat gatgggcggg cagatcggcc tgaacagcac
     1621 tctcgggaag ggcagcacat tctggttcga cctgcccttc aagaagcaga tgacggggca
     1681 ggcagccgag gcttcgttgc ggctcaacga tagccggatc atgctgatag gtttctccga
     1741 cgtggagctg caggccctcg agcaagacct cgccacctgg ggggcgcgca ccgggcgcgc
     1801 ggcgggcgtc gaggcagggg ccgcgcgcct cgccgaagcg cagtcactgg ggcatcccta
     1861 ccacctggtg ctcctgcgcg aagacacgtc gatcgcgccg ggccggaatc tggggcggct
     1921 gcgcaacagc cgcagccaga aggcacctcc cacggtgctg tgcaccgcgg gcgtatcgca
     1981 gattcgcagc gccgaagcgc tcgcgggcgg tttcgtggcg gtgctggaaa tgccgttcaa
     2041 gaaacgcctc ctgttcaacg tcattcattc cgcgactgcg gtccaaggca gccaggaagg
     2101 cgtcatttcg ctcagcgact actacgctac ccgcgacgcc aaccggcgcc gctaccagat
     2161 cctggtggcc gaagacaacg ccgtcaatca gcgcgtc
//
LOCUS       DASQOP010000172         5933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1878854, whole genome shotgun sequence.
ACCESSION   DASQOP010000172 DASQOP010000000
VERSION     DASQOP010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5933
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1878854"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VE030_06920"
     CDS             <1..223
                     /locus_tag="VE030_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23179.1"
                     /translation="AINQHATSAEVPHWAKQMHIFVLEDMGEHETAKLLLGGLLASGA
                     ITDAHEARFLTQRLNAMQGAEKSSQTSGK"
     gene            500..1222
                     /locus_tag="VE030_06925"
     CDS             500..1222
                     /locus_tag="VE030_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HZF23180.1"
                     /translation="MNRKKNQGFTLIEIAIVLVIIGLLLGGILKGQELITSARVRNMI
                     SQQDGVKAAFFGFQDRFRALPGDYTTASINLKCQATCLNGNGNGQIESGAVPVSGSEV
                     HEDLLSWTHLTAANFLNGSYNMTAGDAAATDANSPKNAYSIYMQLIYDDNYADGGTAV
                     RKHNLKTGNQVPVEILAEVDRKIDDGNPLQGGFRFSRYTPASPTGLPAPAAAYAAGVC
                     ASTTGTWFATNGETNCGAASLF"
     gene            1395..2096
                     /locus_tag="VE030_06930"
     CDS             1395..2096
                     /locus_tag="VE030_06930"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HZF23181.1"
                     /translation="MSSRQTQGFTLVEIAIALIIIGLLLGSILRASELITSARVRGLI
                     SQQDGIRAAYYGFLERFREIPGDYSQATFNIAGATQNGNGNGQIEISTAPNETILAWE
                     HLSRAGFLAGNYTYNATESDTTSPKNRYGIYLQLVYDGNYGGGTVATPNPLMHNIKSG
                     SQIPVEIVAEMDRKIDDGAPNAGTFQFSRYQGNGSAAPTDGTTAAPACTSATTPTGIW
                     NSTNGSSNCGGASLL"
     gene            2166..3146
                     /locus_tag="VE030_06935"
     CDS             2166..3146
                     /locus_tag="VE030_06935"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZF23182.1"
                     /translation="MVENSGRTLVCSVLFIDIVEYSKQSVAEQLQFKQAFNRVLGGAL
                     EAVAPRDRVLIDTGDGAAVAFLGDPEDALFVALAIRDAHLPVRMGINLGPVRLVKDLN
                     GQVNIIGDGINVAQRVMSFSEPGQLLVSRSFYEVASRLSNDYAELFTHEGSRTDKHVR
                     EHEVYSVTVDSRADRRIATESRRQTRGNADRQSPADALLTTPGVNENIANLPAHVFDA
                     GANLIISGYSRSSVQDALNKLADSGARVISPITQVSNKWMATCEHPNVPVSACKVETL
                     GHRRIVTGPTREAVSAKVEELVQFGAILVRDIQSANGVWIAICETDGPVR"
     gene            3287..4276
                     /locus_tag="VE030_06940"
     CDS             3287..4276
                     /locus_tag="VE030_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HZF23183.1"
                     /translation="MTALGKIAPAQAIALGRRVLEIEARAVVKLSERLDDSFVRALEI
                     VLGCSGRIVVSGIGKSGHIARKIASTLASTGTPAFFVHPAEASHGDLGMVTRDDVMIA
                     LSNSGESAELLVIVPLIKRQGARLIAITGNPRSTLAAEADVHLDANVAEEACPLGLAP
                     TASTTAALALGDALAVALLEARGFGEEDFARSHPGGSLGRRLLTHVSDVMRSAEAIPR
                     VSDNASLSEAVLEMSKKGMGMTAIVDHGDCLVGIFTDGDLRRTLDKLGDLRKQKVSAV
                     MTPRPRTIRPNQLAVEAVQLMEEYKITQLLAVDTDMRLVGALNMHDLMRAKVI"
     gene            4324..4851
                     /locus_tag="VE030_06945"
     CDS             4324..4851
                     /locus_tag="VE030_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZF23184.1"
                     /translation="MNKRLISRARRIRLAIFDVDGVLTDGALHLGKHGEQIKTFNILD
                     GLGLKMLAASGVGLALLSGRKSPMVAQRARETGIAHVLQGRTDKLEAYHGLLRKLKLA
                     EEETSFMGDDLPDLPVLRRCGLAFSVPDALPIVRSHAHYVTENAGGRGAVREACEFLM
                     RAQGTLEAQLATYLR"
     gene            4848..5447
                     /gene="lptC"
                     /locus_tag="VE030_06950"
     CDS             4848..5447
                     /gene="lptC"
                     /locus_tag="VE030_06950"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HZF23185.1"
                     /translation="MNDRLVAWSPLILLALLAALTFWLDRKVQPPARLPDGSTRHDPD
                     FYVEGFSAMKMNPDGTRRYALSAKRMVHYPDDNSTHLELPRLVYFDYERAPVTVRSDT
                     AEVAHGGDEVFFNGDVQVVRSAYASNAELGMFTSFLHVIPDKDLAKTDKPVRMVEGNS
                     TASSVGLEFDNRSRQIKLLSEVKASYEVPRRVQKRPSRP"
     gene            5419..>5933
                     /gene="lptA"
                     /locus_tag="VE030_06955"
     CDS             5419..>5933
                     /gene="lptA"
                     /locus_tag="VE030_06955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HZF23186.1"
                     /translation="MYKRDLPDRRLARALVWAALACAMNAPPAVWAERADREKPVNLE
                     ADRVDLDDAKKEAVFEGNVTLTQGTLMIKADKIIVKQDADGFQYGIAYGGPAHFRQKR
                     EGFDDFIEGFSERLEYDGKADKVQMFGNARIQRGRDEVRGDYISYNAITEFFQVIGGG
                     KTVATPGNPQGR"
BASE COUNT         1253 a         1827 c         1785 g         1068 t
ORIGIN      
        1 cgcaatcaac cagcatgcca cctcggctga ggttccccac tgggccaagc aaatgcacat
       61 ttttgtgctc gaagacatgg gcgaacacga gaccgccaag ctccttctcg ggggcttgct
      121 ggcaagcggc gcgatcaccg acgcgcacga ggcccgcttc ctcacccagc gcctgaacgc
      181 aatgcaaggt gctgaaaaat cgtcacaaac gtcgggaaaa tgactctttg cgaaaaaccc
      241 atgccgggtg cggcagtcca ctccagcccc ggttgcgggc ttctgaggtg acaataaaat
      301 tgatgctaat cacatagata tgaaaaaagc atcaatccac aactgcaccg cccaccctac
      361 gactgcagct cgggcacagt ctcggcagaa tactagtagc ttattgtaaa tactatgatt
      421 ttctgattac ctgccccgtt ggcacggttc tagcctgaca aggctcacca aaacgttcct
      481 catatggagg ttcacgatca tgaaccgaaa aaaaaatcag ggctttaccc tcattgaaat
      541 cgccatcgtg ctggtcatca tcggcctgct gctcggcggc attctcaagg gccaggaact
      601 catcactagc gcccgtgtgc gcaacatgat ctcccagcag gacggggtca aggctgcgtt
      661 cttcggcttc caggatcggt tccgtgcgct tccaggagac tatacgaccg catccatcaa
      721 cttgaagtgc caggcgacct gcctcaatgg caacggcaat ggccaaatcg aatctggcgc
      781 ggtccccgtc agcggcagcg aggttcacga agaccttttg tcatggacac atctgacggc
      841 ggcaaacttc cttaacggaa gctacaacat gaccgccggc gacgcagcgg ccaccgatgc
      901 caatagcccg aagaatgcgt acagcattta catgcaactg atttacgacg acaactatgc
      961 cgacggcggt accgcagtca ggaaacataa tctcaagacc ggaaaccagg tgcccgtcga
     1021 gattctcgct gaagtggatc gcaaaattga cgacgggaac ccgctgcagg gtggcttccg
     1081 tttctcccgg tacacaccag catctccgac aggcctcccg gcgcccgcgg cagcgtacgc
     1141 cgccggtgtc tgcgcaagta ctaccgggac gtggttcgcc accaacggcg aaacgaactg
     1201 tggtgcggcg agtctgttct aataggtcga atggcactcc ggaaggcccg ccttgcgcgg
     1261 gctttctttt tggggtctag ccctgcgtca gaagcatcct tgcgaccgcc aggcgtggca
     1321 tctaccgacc atggttgtgg cgaaaaatcc ggcgtcgcga tacgccgcga gcttcatatt
     1381 ggccagcccc agcgatgagt tcaaggcaaa cacaagggtt cacactcgtc gagatcgcta
     1441 tcgcgctgat catcatcggc ctcctgctcg gtagcattct ccgggcatcg gaactgatca
     1501 ccagcgcgcg cgttcgcggt ctgatttcgc aacaggacgg tatcagggcc gcgtactacg
     1561 gcttcctgga gcgtttccgc gagatcccgg gtgactactc gcaagcgacg ttcaacattg
     1621 ccggcgccac ccagaacggc aatggtaacg gccagattga aatatccacc gctccgaacg
     1681 agaccatttt ggcgtgggaa catctgtcac gggcgggttt cctggctgga aactacacct
     1741 acaatgcgac ggagtccgac acgaccagtc cgaagaatcg atatggaatc tatctgcagc
     1801 tcgtatacga cggcaattat ggaggcggaa ctgtggccac gccgaatccg ctcatgcaca
     1861 acatcaagag cggctcacag attccggtgg agatagtggc ggaaatggac cgcaagattg
     1921 acgatggtgc gcccaacgcc gggactttcc aattctcccg atatcaaggc aacggcagcg
     1981 ccgcacctac cgacgggacg accgccgctc ccgcttgcac cagcgcgaca acgccaaccg
     2041 ggatctggaa ctccaccaac ggcagcagca actgcggcgg cgccagcctg ctgtaaactc
     2101 caacggagtc cgcggtagaa tgactgccgc ttcatgctca cattttccct ttcggcagcc
     2161 tagcaatggt ggagaattcc ggccgcacgc tggtgtgcag cgtgctgttt atcgacatcg
     2221 tcgagtactc aaaacagtcg gttgccgagc agctgcagtt caagcaggca tttaaccgcg
     2281 tgctcggcgg cgccctcgaa gcggtcgcgc cgcgcgaccg ggtgctgatc gacaccggcg
     2341 acggcgcggc ggtagccttc cttggcgacc ctgaggatgc gctcttcgtc gccctcgcga
     2401 tccgtgatgc tcacctgccg gtccgcatgg gaatcaacct cggcccggta cgcctggtga
     2461 aggacctcaa cggccaggta aacatcatcg gcgacggcat caacgtcgcg cagcgggtga
     2521 tgagcttctc cgagcccggg cagctactgg tgtcgcgctc gttctacgag gttgcgtccc
     2581 ggctgtcgaa tgactacgcc gaactgttca cgcacgaggg ctcgcgcact gacaagcatg
     2641 tgcgcgagca tgaggtgtac tcggtcaccg tggattcacg ggccgatcgc cgtatcgcaa
     2701 ccgaatcacg gaggcaaacg cggggcaacg ctgatcgtca atccccagcg gatgccctgc
     2761 tcacgacgcc cggtgttaat gaaaatattg caaatctgcc tgctcatgtt tttgatgcag
     2821 gagcaaacct catcatctcc ggatatagca gatcaagcgt tcaggatgca ttgaacaagc
     2881 tcgcggatag cggagcgcga gtgatttcgc caatcacgca ggtcagcaat aagtggatgg
     2941 cgacctgcga acacccaaat gtgcccgtaa gtgcctgcaa agttgaaaca ttgggacaca
     3001 ggcgaatcgt gactggaccg acccgcgaag ccgtctctgc gaaagtcgaa gaattggtcc
     3061 agttcggcgc catcctcgtc cgcgacatac aatctgcaaa cggcgtgtgg atagcaattt
     3121 gcgaaaccga cgggcccgtg cgttaattgc gaactgccgc agcagaaaat tcgcgcgaac
     3181 ggttttttcc tagccctgca tcaggaactc taacacagga cttttccggc tcttcgagat
     3241 gcacgcggag gggctcgccc gatgccatgg gttatactcc gtcaccatga cggcactggg
     3301 aaagatcgca ccggcgcagg cgatcgcatt gggtcggcgg gtgctcgaga tcgaggcgcg
     3361 cgccgtggtg aagctgtcgg agcggctcga tgacagtttc gtgcgcgcac tcgaaatcgt
     3421 gctcggttgc agcggtcgca tcgtggtgag cggaatcggc aagtccggcc acatcgcccg
     3481 caagattgca tccaccctgg cgagtaccgg cactccagca ttcttcgtcc atccggccga
     3541 agccagccac ggcgatctcg gcatggttac gcgcgacgac gtgatgatcg ccctgtcgaa
     3601 ttcgggagag agcgccgagc ttctggtgat cgtcccgctg atcaagcgcc agggcgccag
     3661 gctgatcgcc atcaccggca atccgcgctc gacgctggcc gccgaggccg acgtccatct
     3721 cgacgccaac gtcgccgagg aagcctgccc gctcgggctc gcgccaaccg ccagcaccac
     3781 ggcagcgctc gcgctcggcg atgcgctcgc ggttgcgctg ctggaagcgc gcggattcgg
     3841 cgaggaagat tttgcccgct cccatcccgg cggcagcctc ggccggcgtc tgctcaccca
     3901 cgtctcggat gtgatgcgca gcgctgaggc gattccgcgc gtgtcggata acgcgtcgct
     3961 ttccgaggcg gtgctggaga tgtcgaaaaa aggaatgggc atgacagcca tcgtggatca
     4021 cggcgattgt cttgtcggca tcttcaccga cggcgacctg cggcgcacgc tggacaaact
     4081 cggcgatctg cgcaagcaga aagtgagcgc agtcatgacc ccccgcccgc gcacgatccg
     4141 gcccaatcag ctcgccgtgg aagcggtgca gctcatggag gagtacaaga tcacccagct
     4201 gcttgctgtg gacaccgaca tgaggctggt tggcgcgctc aacatgcacg acctgatgcg
     4261 ggcaaaggtc atatgagagt ggtcgcgtcc ccgcggcgct gtgaccggcg aggaccgtga
     4321 ccggtgaaca aacggctgat ctcgcgcgct cgtcgcatcc gtctagcgat tttcgacgtt
     4381 gacggcgtgc tgaccgacgg cgcgctgcat ctcggcaagc acggcgagca gatcaagaca
     4441 ttcaacatcc tcgacggcct cgggctgaaa atgctggctg caagcggtgt aggattggca
     4501 ttgctgtccg ggcgcaagtc acccatggtc gcgcagcgcg ccagggaaac gggcatcgcg
     4561 cacgtgctgc aaggcagaac cgacaagctc gaggcctatc acggcctgct gcgcaagctc
     4621 aagctcgccg aagaggaaac ttcgttcatg ggggacgatc tgcccgacct gccggtgttg
     4681 cgccgatgcg gcttggcttt cagcgtgccg gacgcgctgc cgatcgtccg cagccacgcc
     4741 cactatgtca ccgagaacgc ggggggccgc ggagcggttc gcgaagcctg cgagttcctg
     4801 atgcgcgcgc agggaaccct ggaagcccaa ctggcgactt acctgcgatg aacgacagac
     4861 tggtagcctg gtctccgctg atcctgctgg cgctgctcgc ggcgctgacg ttctggctcg
     4921 atcgcaaagt ccagccgccg gcgcggctgc ccgacggcag cacgcggcac gacccggatt
     4981 tttacgtcga gggcttctcg gcgatgaaga tgaacccgga cggaacgcgc cgctacgcgc
     5041 tgtcggcaaa acgcatggtg cactacccgg acgacaacag cactcacctg gagctgccgc
     5101 gcctggttta cttcgactac gagcgcgccc cggtgacggt gcgttccgat accgcggaag
     5161 tggcacacgg cggggatgag gtgtttttca acggcgacgt tcaggtggtg cgctctgcct
     5221 acgcaagcaa cgccgagctc ggcatgttca ccagcttcct gcatgtgatt cccgacaagg
     5281 atctcgccaa gaccgacaag ccggtgagaa tggtggaagg aaattccacg gccagctcgg
     5341 ttggcttaga attcgataat cggagccgcc agatcaagct gttgtcggag gtcaaggcca
     5401 gttatgaagt tcccaggcgt gtacaaaaga gaccttcccg accgtaggct ggcgcgagcg
     5461 ctggtctggg ccgcgctcgc ctgcgccatg aatgcgccgc ccgcggtgtg ggccgagcgc
     5521 gcggatcgcg aaaagccggt caatctggag gccgaccgcg tggacctcga cgatgccaag
     5581 aaagaggcgg tgttcgaggg caacgtgacg cttacgcagg gtacgctgat gatcaaggct
     5641 gacaagatca tcgtcaagca ggatgccgac ggcttccagt acgggattgc ctacggcggc
     5701 ccggcccact tccggcagaa gcgcgaaggc ttcgacgatt tcatcgaggg cttttccgag
     5761 cgcctcgaat acgacggcaa ggccgacaag gtgcagatgt tcggcaatgc acgcatccag
     5821 cgcggccgcg acgaagtgcg cggggactac atttcctaca acgcgatcac cgagttcttc
     5881 caggtcatcg gcggaggcaa gacggtggcc acgccgggca atccgcaggg ccg
//
LOCUS       DASQOP010000173         8741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1078609, whole genome shotgun sequence.
ACCESSION   DASQOP010000173 DASQOP010000000
VERSION     DASQOP010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8741
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078609"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..431)
                     /locus_tag="VE030_06960"
     CDS             complement(51..431)
                     /locus_tag="VE030_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23187.1"
                     /translation="MSNAPVGRSFDEMVQSRVGREAAFAEALLQMAVQEMLAGDLDVA
                     RSLIRDVIKGSIGHAELSRRTGTPEKSLVRMFGPSGNPTAANIFSVLAQLQRASGVSL
                     QVKSVRAQGKRSIKPRSGSARRAA"
     gene            complement(598..1194)
                     /locus_tag="VE030_06965"
     CDS             complement(598..1194)
                     /locus_tag="VE030_06965"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HZF23188.1"
                     /translation="MFTGIIEAIGRVAAVKPAGQSAALTIDAGGLNMSDVAVGDSIGC
                     NGVCLTVTSLVPKGFTVDVSQETLRVTAGFAAGNAINLEKSLRLSDRLGGHLVSGHVD
                     GAGTVTAAKPVDANRELIVRYPTELGRYVARKGSIAVNGVSLTVNVVTTDTFSVNLIP
                     HTLAVTNLKDLKQGDRVNLEIDLVARYVERMFDRDDQR"
     gene            complement(1198..2304)
                     /gene="ribD"
                     /locus_tag="VE030_06970"
     CDS             complement(1198..2304)
                     /gene="ribD"
                     /locus_tag="VE030_06970"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HZF23189.1"
                     /translation="MPFSADDFKFMAHALRLAERGLYTTTPNPRVGCVIVKDGRIVGE
                     GWHERAGGPHAEVVALTAGGANAQGATAYASFEPCSHHGRTPPCADALIAAKVARVVA
                     AMQDPNPQVAGSGLRKLREAGIAAEAGILETEARELNIGFVSRMTRGRPWMRVKIAAS
                     LDGRTALKNKVSQWITGADARRDGHRWRARSCAVMTGIGTLTDDDPRLTVRDVETSRQ
                     PLRIVVDSRLRITPEAKILEGEGVLVATATRDEDKNRALARKGAEIVVLPNAEHKVDL
                     LDLAKFLAGRGINEVLVEAGLNLNSALLRAGVVDELLIYLAPHMLGDAARGMFDLGEL
                     TEMNKRLQLNIQETRMIGPDLRILARPVVLNPGA"
     gene            complement(2309..2782)
                     /gene="nrdR"
                     /locus_tag="VE030_06975"
     CDS             complement(2309..2782)
                     /gene="nrdR"
                     /locus_tag="VE030_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HZF23190.1"
                     /translation="MKCPFCKHPDTQVVDSRVNEEGDSIRRRRRCPSCDKRFTTYENV
                     ELRMPQIIKSTGYREEFEEEKLRVGFTRALHKRPVSADLVDDAIERVKQKLLSLGQRE
                     VESRRIGEMVMRELYKLDKVAYIRFASVYRAFQDVEDFRDAVKEVQKPAARRKSK"
     gene            complement(2877..4124)
                     /gene="glyA"
                     /locus_tag="VE030_06980"
     CDS             complement(2877..4124)
                     /gene="glyA"
                     /locus_tag="VE030_06980"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HZF23191.1"
                     /translation="MFPADQNIQDFDPELWRAMEAERQRQEDHIELIASENYTSPRVL
                     QAQGSVLTNKYAEGYPGKRYYGGCEYVDIAEQLAIDRAKTLFKAEIANVQPHSGSQAN
                     QAVYAAALQAGDTILGMSLAHGGHLTHGSPVNLSGKLYHAVSYGLTANEEIDYDQVAH
                     LAAQHNPKMIVAGASAYSLTIDWQRFRDIADSVGAYLFVDMAHYAGLVAAGLYPSPVG
                     IADFVTSTTHKTLRGPRGGIILANVEHEKLINSAIFPGIQGGPLMHVIAAKAVALKEA
                     MAPEFRKYQAQVIANARTMTKTLQSRGLRIVSGRTDCHMFLVDLRAKKITGKDAEAAL
                     GRANITVNKNAIPNDPEKPFVTSGVRIGSPAMTTRGFQELEAEKLSNLIADVLEAPND
                     EANIVRVGSEVVAMCQKFPVYAK"
     gene            4328..6412
                     /locus_tag="VE030_06985"
     CDS             4328..6412
                     /locus_tag="VE030_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HZF23192.1"
                     /translation="MPKTILARFVWLGIAILGASAIGGIAIHRGESINAIWFVVAAAC
                     TYLIAFRFYSAWICAKVLALDETRATPAERFNNGRDFVPTNRWVVFGHHFAAIAGPGP
                     LIGPTLAMQFGYLPGTLWILVGAVLGGCVQDMVIMFCSTRRDGRSLGQMARDELGPVG
                     GWAAMIGTMTIMLILIAVLGLVIVNAMKHSPWATATVLGTIPVAMLIGIYMRGIRPGR
                     VLEGSIIGIVLLILCVLGGGWVDHNETLRGLFDWSGESVAWFVMIYGFAAATLPVWLL
                     LAPRDYLSTFLKLGVVFLLAISILVLAPEMKMPALTQFIDGTGPIFKGTLFPFVFITI
                     ACGAISGFHSLVSSGTTPKLLQNERDIRMIGYGSMALESFVGIMAVIAACVLEPGVFF
                     AINSPAGVVGKEAAAAVATISSWGFPVTVEQMQILAKEMGEATLFARTGGAPSLAVGM
                     ATIFSTTFGGTLLAMWYHFAIMFEVLFILTTLDAGTRVGRFMLQDMLKYFWKPMGNVS
                     SYPMTVISSLIFVTAWGYFLWQGVKDPLGGVNILWPLFGISNQLLAGIALSVATGIII
                     KMGKLRYAWVTGLPLTWLAIICTWAAWEKVFSPNPAIGFLSGAASMAEKLAAGTLSPE
                     RAAVAPQLIIGQRIDAALAFLFAVILWVVIVDMLRMSMRMRSGKPVLPLSESKYIRTQ
                     LDESMISTGQHA"
     gene            6420..6602
                     /locus_tag="VE030_06990"
     CDS             6420..6602
                     /locus_tag="VE030_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HZF23193.1"
                     /translation="MIGAELKQIWRYLRQVSGDDAYERYLAHHRQAHPGEAPMTQEQY
                     FRKRQDEKWSKVSRCC"
     gene            complement(6608..6886)
                     /locus_tag="VE030_06995"
     CDS             complement(6608..6886)
                     /locus_tag="VE030_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23194.1"
                     /translation="MKEKIAAIRKALSKSFNAAPVEYVNADGRHRFNLELPKVTHRVH
                     FSLEVLDGHQATEFKRLCKGASNYLQRNPTGLPKQLFVTDTGVLEEKS"
     gene            7136..7723
                     /locus_tag="VE030_07000"
     CDS             7136..7723
                     /locus_tag="VE030_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23195.1"
                     /translation="MRILLLLLLLPATALALDNDYVRVTRNAATCLQADMPGCGDRVI
                     VALDDVEIFVGYVKRKLSRGEIMVFERGRAFPPPVGEFFEVVIKPDHPPVLSPPEIIP
                     PEKNALLYDGERFFVFEEKLAPGDTRARHSHSQRVVIQLNKTRLQQWPDGEPEKFVEV
                     PVEQPGFSPPVIHVVKNVGDAPLYGIVIEFKPEKK"
     gene            complement(7808..8206)
                     /locus_tag="VE030_07005"
     CDS             complement(7808..8206)
                     /locus_tag="VE030_07005"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZF23196.1"
                     /translation="MMDFATKRLAQVPDVVAPDGSDVRILARTGRGGMAHFELAPGRA
                     SDAIRHRTVDEIWFVLSGRGEIWRKQGDREEIVALEPGVSLTIPVGTHFQFRATGAKV
                     LEAVGVTMPPWPGDTEAVPVPGKWQSTKSS"
     gene            8339..>8741
                     /locus_tag="VE030_07010"
     CDS             8339..>8741
                     /locus_tag="VE030_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23197.1"
                     /translation="MIPKTRKHTARHRDLGLSAREFSILMSLSTPRKIQDYLNAIPIN
                     HEIGGQTILSVREVLRQRRAHCIEGAFFAACALWVHGEPPLVMHLDCDLSDFPHVIAL
                     FRRSGHWGAISKTNGAPLRYRDPIYRSPRELV"
BASE COUNT         1524 a         2807 c         2656 g         1754 t
ORIGIN      
        1 tgatgaagcc gaaatactgc gggcaccaat tcggttcgcg ctccaaaccc tcacgccgcg
       61 cggcgtgcgg acccggatct cggtttgatc gaccgcttcc cttgagcacg cacagacttc
      121 acctgcagac tcacgccgct cgcccgctgc agttgtgcca acacactgaa tatgttggcg
      181 gctgtcggat tgccgctcgg gccaaacatg cgcaccagac tcttctccgg cgttccggtc
      241 cgccggctca attcggcatg acctatcgaa cccttgatca catcgcgaat gaggctgcgg
      301 gcaacgtcga gatccccggc gagcatttcc tgcacggcca tttgcagcag cgcctcggcg
      361 aaggccgctt cgcggccaac tcgagactgc accatttcgt cgaatgaacg tcccactgga
      421 gcattgctca taatccttgc ctcttaactg tttctgcgcc gcttgaaatc cgcccatcgg
      481 gcctgtgcgg cagcggcgtg aacgtccagt tcaccaaacc agcgttggaa cggttcgcgg
      541 ccctccggcg cgccggccgc cgagggccaa aagggccgtg aataacgtga aatctagcta
      601 ccgctggtca tccctgtcga acatgcgttc gacgtaacgc gccaccaggt ctatttcgag
      661 attgacgcgg tcgccctgct tcaggtcttt cagattggtt accgcgagcg tgtgcggaat
      721 caggtttacc gaaaaagtat cggtcgtcac gacattgacg gtcaggctca cgccgttcac
      781 ggcgattgac cctttgcgcg cgacatagcg gccaagctcc gtgggatagc gaactatgag
      841 ttcccggttg gcatccacag gtttcgccgc cgttaccgtg ccggcgccat ccacatggcc
      901 gctcacaaga tgtccgccga gacggtccga gagcctcagc gacttctcca gattgatggc
      961 attgccggcg gcgaatcccg cggtcacccg caaagtctcc tgcgatacgt cgaccgtgaa
     1021 gccttttggc accagcgaag tgacagtcag gcacaccccg ttacagccga tgctgtcgcc
     1081 gaccgcgaca tcgctcatgt tcagaccacc ggcgtcaatc gtcagcgcgg ccgattgtcc
     1141 ggcaggcttg actgccgcga cgcggccgat agcttcgatg attcctgtga acacttctca
     1201 ggctccggga ttcaagacta ccggccgcgc gagaatcctc aggtccggtc cgatcatgcg
     1261 ggtctcctga atgttcaact gcaggcgctt gttcatttca gtcaattccc ccaggtcgaa
     1321 catgccgcgc gccgcgtctc cgagcatatg cggagcaagg taaatcagca gctcgtccac
     1381 cactccggca cgcagcaatg ccgaattcaa attgagaccg gcttccacca gcacctcgtt
     1441 gatgccgcgc ccggcgagaa acttcgccag atccagcagg tccaccttgt gctccgcgtt
     1501 gggcagcacc acgatttcgg cgcctttcct cgccagcgcg cggttcttgt cctcatcgcg
     1561 cgttgccgtg gccaccagaa caccctcacc ctccagaatc ttcgcctccg gcgtgatccg
     1621 cagccggctg tccaccacga tgcgcagcgg ctggcgcgag gtttccacgt cgcgcaccgt
     1681 gaggcgcggg tcgtcatccg taagcgtgcc gatgccggtc attaccgcgc aggaacgggc
     1741 acgccagcgg tgtccgtcgc ggcgcgcgtc cgctcctgtg atccactggc tgaccttgtt
     1801 cttcagtgcg gtcctgccgt ccaggctggc cgcgattttc acgcgcatcc acggccgccc
     1861 gcgggtcatg cgcgagacga agccgatgtt cagttcgcgc gcctcggttt ccagaattcc
     1921 ggcctcggcc gcgatgcccg cttcgcgcag tttccgcagt ccgcttccgg caacctgggg
     1981 attcgggtcc tgcatcgccg cgaccacgcg cgccaccttc gcggcaatca aagcatccgc
     2041 gcatggcggc gtgcggccgt ggtggctgca aggctcgaat gaggcatacg cggtggcgcc
     2101 ctgcgcgttt gcgcctccgg cggtcaatgc caccacttcg gcatgcgggc ctccggcgcg
     2161 ctcatgccag ccctcgccca cgatccggcc gtccttgacg atcacgcaac cgactcgcgg
     2221 gttcggagtc gtggtgtaga gcccgcgttc ggcgagccgc agcgcgtgcg ccatgaattt
     2281 gaagtcatcg gcactgaagg gcatgcgctt acttgctctt gcggcgcgcg gcgggcttct
     2341 gcacttcctt caccgcgtcg cgaaaatcct cgacatcctg aaaggcgcga tacaccgacg
     2401 cgaagcggat ataggccact ttgtcgagct tgtagagttc gcgcatcacc atctcgccaa
     2461 tgcggcgcga ctccacctcg cgctggccca gcgacagcag cttctgcttg acgcgttcga
     2521 tcgcatcatc gaccaggtcc gcggagaccg gccgcttgtg cagcgcgcgg gtgaaaccaa
     2581 cgcgcagctt ttcttcttcg aactcttcgc ggtaaccggt gctcttgatg atctgcggca
     2641 tccgcagctc gacgttctcg taggtggtga agcgcttgtc gcacgacggg cagcgccgcc
     2701 gccggcgaat gctgtccccc tcctcgttga cccgggagtc gacgacttgc gtatccggat
     2761 gcttgcagaa cgggcacttc atgccgaggg acgaggggcg agggacgagg aacgagtaaa
     2821 ggcacgaagc tctatagccc cctcgtccct cgtccctcgt ccctcgcccc tgctgttcac
     2881 ttcgcgtaca ccggaaactt ctgacacatg gcaacaacct cactcccaac tcgtacgata
     2941 ttcgcttcat cgttcggcgc ttcgagcacg tctgcgatga ggttggagag cttttcggct
     3001 tcgagttcct ggaagccgcg ggtggtcatc gccggcgaac cgatgcgcac accgctggtg
     3061 acgaaaggct tttcgggatc gttggggatc gcgttcttgt tcacggtgat gttggcgcgg
     3121 ccaagagcag cttcggcgtc cttgccggtg atcttcttgg cgcgcaggtc caccaggaac
     3181 atgtggcagt cggtgcgccc cgacacgatg cgaagcccgc ggctctgcag cgtcttggtc
     3241 atggttctcg cgttggcaat cacctgcgcc tggtatttgc gaaattccgg tgccatcgct
     3301 tccttgagtg cgaccgcctt ggcggcgatc acgtgcatca gggggccgcc ctggatgccg
     3361 ggaaagatcg ccgagttgat gagcttttcg tgctcgacgt tggccaggat aatgccgccg
     3421 cgcgggccgc gcagcgtctt gtgggtggtg ctggtaacga aatcggcgat ccccaccgga
     3481 ctcgggtaga ggccggcggc aaccagtccc gcgtaatgcg ccatgtccac gaacaggtag
     3541 gcgccgaccg agtcggcgat atcgcggaag cgctgccagt cgatggtcag agaatacgcc
     3601 gaggcgccgg cgacgatcat tttcgggttg tgctgtgccg ccagatgcgc gacctggtcg
     3661 taatcgattt cctcgttcgc ggtcagcccg taagagaccg catggtagag tttgccggac
     3721 aggttcactg gcgagccgtg ggtgagatgg ccgccgtgcg cgagcgacat cccgagaatg
     3781 gtatcgccgg cctggagtgc ggcagcgtaa accgcctggt tggcctgcga gcccgagtgc
     3841 ggctgcacgt tggcgatctc cgccttgaac agcgtcttgg cgcgatcgat tgccaactgt
     3901 tccgcgatat ccacgtactc gcagccgccg taatagcgct tcccagggta gccttcggcg
     3961 tacttgttgg tgagcaccga gccctgtgcc tgcagcacgc gcgggctggt gtagttttcc
     4021 gaggcgatca gttcgatgtg gtcttcctgg cgctggcgtt ccgcctccat tgcccgccac
     4081 aattctgggt cgaaatcctg gatgttctgg tcagcgggaa acatgatggc ctgagaggga
     4141 aagtgagggc tccggaaagc ggcgaattgt accattccgg tcaaaaggac cccggaatca
     4201 agagcgttga cgcatcgcaa gtccggcttg ctgctattat tggcgggctc atggatacgg
     4261 cgccagacgc ggggccgcaa gcaaacgtcc gcatcgaggc agcctataaa aatctggggg
     4321 agacgacatg ccaaaaacca tcttagccag gttcgtctgg ctgggtatcg cgattctcgg
     4381 tgcgtccgcc atcggcggta tcgccatcca ccgcggcgaa tccatcaacg ccatctggtt
     4441 tgtcgtggcg gccgcgtgca cctatctgat cgccttccgc ttctacagcg catggatttg
     4501 cgccaaggta ctggcgctcg acgaaacccg cgccacgccg gccgaacgct tcaacaacgg
     4561 tcgcgatttc gtcccgacta acaggtgggt ggtattcgga caccacttcg cggcgatcgc
     4621 cgggccggga ccgctgatcg gcccgaccct ggcgatgcaa ttcggctatc tccccggcac
     4681 gctctggatc ctggtcggtg cggtcctcgg cggctgcgtg caggacatgg tgatcatgtt
     4741 ctgctccacg cgccgcgacg gccgcagcct cggccagatg gcgcgcgacg aacttgggcc
     4801 cgtcggcggc tgggcggcga tgatcggcac catgaccatc atgctgatcc tgatcgcggt
     4861 gctgggcctg gtgatcgtca acgccatgaa gcacagcccc tgggcgacgg caacggtgct
     4921 gggcactatt ccggtagcga tgctgatcgg catctacatg cgcggcatcc gccccggccg
     4981 tgtcctcgaa ggatcgatca tcggtatcgt gctgctgatc ctgtgcgtat tgggcggcgg
     5041 ctgggtggac cacaacgaaa cgttgcgtgg gctgttcgac tggagcggcg aatccgtcgc
     5101 ctggttcgtg atgatctatg gtttcgccgc cgcaacgctg cccgtgtggt tgctgttggc
     5161 gccgcgcgac tatctttcca cgttcctgaa actgggggtg gtgttcctgc tcgccatctc
     5221 aatcctggtg ttggcccctg aaatgaagat gccggcgctg acccagttca tcgacggcac
     5281 cggcccgatc ttcaagggca ccttgttccc gttcgtgttc atcaccatcg cctgcggcgc
     5341 gatctccggt ttccattcgc tggtgtcctc gggcactaca ccgaaactgc tgcagaacga
     5401 gcgggacatc cgcatgatcg gctatggctc gatggcgctg gaatccttcg tcggaataat
     5461 ggcggtaatc gcggcctgcg ttctcgagcc gggcgtattc ttcgcgatca acagcccggc
     5521 tggcgtggtc ggcaaggaag ccgcggccgc ggtcgccacc atttcctcgt ggggcttccc
     5581 ggtcacggtc gagcagatgc aaatcctggc aaaagaaatg ggtgaagcca cgctattcgc
     5641 ccgcaccggc ggcgcgccgt cgctggcggt gggcatggcg accatcttct cgaccacgtt
     5701 cggcggcacg ctgctggcga tgtggtacca cttcgccatc atgttcgaag tgctgttcat
     5761 ccttaccacg ctggatgcgg gcacgagggt gggacgtttc atgttgcagg atatgctcaa
     5821 gtatttctgg aaaccgatgg gcaacgtatc ctcctacccg atgacggtga tctcgagcct
     5881 gatcttcgtt accgcctggg gttacttcct ttggcagggc gtcaaggacc cgctcggcgg
     5941 cgtgaacatc ctgtggccgc tgttcggcat ttccaaccag ttgctggccg gcatcgcgct
     6001 gtcggttgcc accggtatca tcatcaagat gggcaagctg cgttacgcct gggtcacggg
     6061 actgccgctg acctggctgg cgatcatctg cacctgggcg gcgtgggaga aagttttcag
     6121 tcccaacccc gcaatcggct tcctgtccgg cgccgccagc atggccgaaa agctcgccgc
     6181 cggcacgctg tcaccggaac gggccgcggt ggctccgcag ttgatcatcg gccagagaat
     6241 cgatgccgcg ctggccttcc tgttcgccgt cattctgtgg gtggtgatcg tcgacatgct
     6301 gcgcatgtcc atgcgcatgc gctccggcaa gccggtgctg ccgttgtccg agtcgaagta
     6361 catcaggacg cagctggacg agagcatgat ttccacgggg cagcacgcct gaggccacga
     6421 tgatcggcgc cgaactcaag caaatctgga gatacctgcg gcaggtcagc ggcgacgacg
     6481 cttacgagcg ctaccttgcg caccatcggc aggcgcatcc cggcgaagcg ccgatgacgc
     6541 aggagcagta cttcaggaaa cgtcaggacg agaagtggag caaggtgtcg cgctgctgct
     6601 gagaaattca ggacttctcc tcgagaacac cggtgtccgt gacgaataat tgcttcggca
     6661 atcccgtggg attgcgctgc aggtagtttg aagccccttt gcacagccgc ttgaattccg
     6721 tggcctggtg cccgtcaagg acctccagcg agaagtgcac ccggtgcgtg accttcggga
     6781 gttccaggtt gaacctgtgg cgtccgtccg cattcacgta ctcgacgggc gcagcgttga
     6841 aactcttgct cagtgccttc cgaattgccg caatcttttc tttcatatgg cctctaaagg
     6901 tagcccctcg gcgcggaccc gcggactggg gatacttacc ccctaccggc cgcacctcga
     6961 tcatgtatcg ccctccacta taggcttccg ggaggctctt taggcactaa atgacgtgac
     7021 gagacgggga catgccccat cactcgctcg gcgcgcgccg gtggtaaagt gcatttggcc
     7081 ccgatagatg cgcgttcgac gctcccgcga cgaccaattc cgaccgaggt gcttcatgag
     7141 aattttgctg ctgttgcttc tcctgccggc caccgcgctg gcactggaca acgactatgt
     7201 gcgggtgacg cgcaatgcgg cgacctgcct gcaggccgat atgcccggct gcggcgaccg
     7261 ggtgatcgtg gcgttggacg acgttgagat cttcgtcggc tacgtcaaac gcaaactctc
     7321 gcgcggcgag atcatggtgt tcgaacgcgg aagggccttt ccgcccccgg taggggagtt
     7381 tttcgaagtc gtcatcaagc ccgatcaccc accggtgctc tcgccgccgg aaatcatccc
     7441 gccggaaaaa aacgccctgc tttacgacgg cgaacgcttc ttcgtattcg aggaaaagct
     7501 tgcgcccggg gacacgcggg cgcgccacag tcacagccag cgcgtggtga tccagctcaa
     7561 caagacgcgc ctgcagcagt ggcctgacgg cgagccggag aagtttgtcg aagtgccggt
     7621 cgaacagccc ggtttcagtc cgccagtgat ccacgtggtg aaaaatgtcg gcgacgctcc
     7681 gctgtacggc atcgtgatcg agttcaagcc ggagaaaaag tagccgcggg tcgcggatag
     7741 gctggaggtc tggacacgac gcgaaatccg cgttcgcaaa gccggcgcaa acggggcgct
     7801 tgcgctctca ggaagatttc gtcgattgcc acttgcccgg caccggcacg gcttcggtgt
     7861 cgcccggcca cggcggcatg gtgacgccga cagcttccag caccttggcg ccggtcgcac
     7921 gaaactggaa atgcgttccg accgggatcg tcagactgac gcccggctcc agtgcgacga
     7981 tttcttcgcg gtcgccttgc tttcgccaga tctcgccgcg gcccgacagc acgaaccaga
     8041 tttcgtccac cgtgcgatgc cggatggcgt ccgaggcgcg ccccggcgcc agttcgaagt
     8101 gagccatgcc gccccgtccg gttctcgcca gtattcggac atccgacccg tcgggtgcaa
     8161 caacatcagg cacttgcgcc aggcgcttgg tcgcgaaatc catcatctat gccgatcctt
     8221 cctggtatcg ctgttcgtcg gccaactgct taggtagtat ttccgcactg tcgctccata
     8281 acaggatacc gtccgtggct cagaccccgc ttcccggtgg ttctctacga accgtggcat
     8341 gattcccaaa acccgcaaac atactgcacg ccaccgcgat ctcgggctca gcgcgcgcga
     8401 gttctcgatc ctgatgagct tgtccacgcc ccggaaaatc caggattacc tgaacgcgat
     8461 tccgatcaat cacgaaatcg gcggacagac catcctgtcg gtgcgcgaag tactcaggca
     8521 gcgccgcgcg cattgcatcg aaggcgcctt tttcgccgct tgcgcgctct gggtgcacgg
     8581 cgagccgccg ctggtgatgc acctggactg cgatctgagc gacttcccgc acgtcattgc
     8641 gttgttccgt cgcagcggcc actggggcgc gatctccaag accaacggcg cgccgctgcg
     8701 ttaccgcgac ccgatctatc gcagcccgcg cgaactggtc a
//
LOCUS       DASQOP010000174         3373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2286537, whole genome shotgun sequence.
ACCESSION   DASQOP010000174 DASQOP010000000
VERSION     DASQOP010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2286537"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VE030_07015"
     CDS             complement(<1..323)
                     /locus_tag="VE030_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF520 family protein"
                     /protein_id="HZF23198.1"
                     /translation="MPSFDIVSEVNQVELRNAIDQTNKEISNRFDFKGSDARVELADK
                     ALTVFADDDFKIAQVYDVLIARMAKRNVDVRALQRAKSEKIGGDKVKQVITVKVGVDQ
                     ELGKK"
     gene            complement(393..1634)
                     /locus_tag="VE030_07020"
     CDS             complement(393..1634)
                     /locus_tag="VE030_07020"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HZF23199.1"
                     /translation="MTKKASTRPIRKVVLAYSGGLDTSVILKWLQDTYGCEVITFTAD
                     IGQGEELEPARRKARKFGVKQIFIEDLREEFVRDFVFPMFRANAVYEGEYLLGTSIAR
                     PLIAKHLVEIARKTGADAISHGATGKGNDQVRFELGAYALMPDVKVIAPWREWDLLSR
                     EKLLAYAEKHGIPVDFKKRKGGGAPYSMDANLLHISYEGGILEDPDFEPEESMWRITV
                     SPEKAPNRPEHIDLTYRRGDIVAVNGSRMTPAQVLAKLNQLGGKHGIGRLDIVENRYV
                     GMKSRGCYETPGGTIMLRAHRAIESITLDREVLALKDDLMPRYARMIYNGYWFSPERV
                     LLQSLIDNSQQTVNGTVRLKLYKGTVLVVGRASKTDSLFDSRIATFDDDRGAYNQADA
                     AGFIRLIALRLRIGARRNKGK"
     gene            complement(1875..2387)
                     /locus_tag="VE030_07025"
     CDS             complement(1875..2387)
                     /locus_tag="VE030_07025"
                     /inference="COORDINATES: protein motif:HMM:NF014543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23200.1"
                     /translation="MIDRIVIPRIFLLLALVLPTFGTMAAGDEDVAWIADSRGCKVAN
                     PFPQPGETIKWSGSCRNGLADGNGLLEWFIDGKPADRYEGPLKQGWAEGKGTLLREGG
                     RYAGDWKRSLQDGNGRYDAADGSWYEGEWKDGQPHGHGQYQTPDGRLFSGTWENGEYQ
                     GDMENDPNRT"
     gene            complement(2489..>3373)
                     /gene="argF"
                     /locus_tag="VE030_07030"
     CDS             complement(2489..>3373)
                     /gene="argF"
                     /locus_tag="VE030_07030"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HZF23201.1"
                     /translation="EYLFGRTRWIKQQFKAYQPHNPLFDRTLVMIFEKQSTRTRLSFE
                     AGMHQLGGSAIFLSTRDSQLGRGEPVEDAAQVISRMCDIVMVRTFEQEIIERFAAHSR
                     VPVINGLTNEYHPCQILADIYTFIERRGPVRGKTVAWIGDSNNVCNTWLQAAEVLGFN
                     VHVSTPPGYEVEPERAGLYGTDHYEEFDDPMEAARGADLVTTDVWTSMGFEAENEERK
                     RDFEDWQVDAEMMRLASPDALFMHCLPAHRGEEVAGDVIDGPQSVVWEEAENRLHTQK
                     ALMEYLLLGRMEENRKSK"
BASE COUNT          545 a         1065 c         1007 g          756 t
ORIGIN      
        1 atcttcttgc cgagctcctg gtccacgccg accttgacgg tgatcacctg cttcaccttg
       61 tcgccgccga ttttctcgga cttggcgcgt tgcagcgcgc gtacgtccac gttgcgcttg
      121 gccattctgg caatcaacac gtcatagacc tgcgcgatct tgaagtcatc gtcggcaaac
      181 acggtcagcg ccttgtctgc gagctcgacg cgggcgtccg aacccttgaa gtcgaaacga
      241 ttggaaattt ccttgttggt ctggtcgatg gcgttcctca gctcgacctg gttgacctcg
      301 gaaacgatgt cgaaactggg catgatgatt tccctcccgg cttggtgttt cttcggctgc
      361 ccgcgggcgc cccacggtag cccggccagc cgctatttcc ccttattgcg cctggctcca
      421 atgcgcaggc gcagcgcgat cagccggatg aagccggccg catcggcctg attataggca
      481 cccctgtcgt catcgaaagt ggcgatcctc gagtcgaaca gggaatccgt cttcgaggcg
      541 cgcccgacga ccaataccgt gcccttgtac agcttgagcc gcaccgtgcc gttcacagtt
      601 tgttgcgagt tatcgatcag cgattgcagc agcacgcgtt ccgggctgaa ccagtagccg
      661 ttgtagatca tgcgcgcata acggggcatc aggtcgtcct tcagcgcgag cacttcccga
      721 tccagcgtga tcgattcgat tgcccggtgc gctctgagca tgatggtgcc gcccggggtc
      781 tcgtagcaac cgcgcgattt catgccgacg tagcggtttt cgacgatgtc gagccggccg
      841 atgccgtgtt tgccgccgag ctggttgagc ttggcgagca cctgcgccgg cgtcatcctg
      901 ctgccgttca cggcgacgat gtcgcccctg cggtaggtca ggtctatgtg ttcgggccgg
      961 ttcggcgcct tttccggaga gacggtaatg cgccacatcg actcttcggg ctcgaagtcc
     1021 gggtcctcga gaatcccgcc ctcgtaggaa atgtgcagca ggtttgcgtc catcgaatag
     1081 ggggcgccgc cgcctttcct cttcttgaaa tccaccggga tcccatgctt ttcggcatac
     1141 gccagcagct tctcgcgcga cagcaggtcc cattcgcgcc acggagcgat cacttttaca
     1201 tccggcatca acgcgtaggc gccgagttcg aagcgcacct ggtcgttgcc cttgccggtt
     1261 gcgccgtggg agatcgcgtc tgcgccggtc ttgcgcgcga tctcgaccag gtgcttggcg
     1321 atcagcgggc gcgcgatcga ggtgccgagc aggtattcgc cctcgtacac ggcgttggcg
     1381 cggaacatcg ggaagacgaa atcgcgaacg aattcttcgc gcagatcttc gatgaatatc
     1441 tgcttgacgc cgaacttgcg ggctttccgg cgtgccggct ccaactcctc accctggccg
     1501 atgtcggcgg tgaaggtgat gacttcgcag ccgtaggtgt cctgcagcca cttcaggatc
     1561 accgatgtgt ccagcccgcc ggagtaggcg agcaccacct ttctgatggg tctcgttgat
     1621 gctttcttcg tcacgttgtc tttagtgtcc tgttccgtta attcgtggct gaaaacccgg
     1681 cgagaatcga cgccatgctg tgttacgagt cctcgccgtg ggacccacca ctgtcgagga
     1741 ctcgcgcctt gcctgacgcc gatttcgcga gttttccaat tgatggtgga ttgctgtcaa
     1801 atagtttata gaccgggttt ccaaggttcg ggagttatcc cacgaattag cggaacagga
     1861 cactaggtcc tttctcaagt cctgttggga tcgttttcca tatcgccctg gtactcgccg
     1921 ttctcccagg tgccgctgaa caggcgtccg tccggcgtct ggtactgtcc atgcccgtgc
     1981 ggctggccat ccttccactc gccctcgtac cagctgccgt cggctgcgtc gtagcggccg
     2041 ttgccgtcct ggaggctgcg cttccagtcg ccggcatagc ggccgccttc gcgcagcagc
     2101 gttcccttgc cctcggccca gccttgcttg agcgggcctt cgtagcggtc ggccggtttg
     2161 ccgtcgatga accattccag caggccgttg ccgtcggcca ggccgttcct gcaggaaccg
     2221 ctccatttga tggtttcgcc cggctgcggg aacggattgg cgaccttgca gccgcggcta
     2281 tcggcgatcc aggcgacatc ctcgtcgccg gcggccatcg taccgaaggt gggaagcaca
     2341 agtgccagca gcagaaaaat gcggggtatg acgattctgt cgatcatgtt cggctcctgt
     2401 gagtaatttg cgttgttccg gatcgcgatg tctgtatgcg ccgcgaccgc ctgtccccct
     2461 gcccggctgc tgccaaaaac cgttctgtct attttgattt ccgattttct tccatgcgac
     2521 ccagaagcag gtattccatc aacgctttct gggtatgcaa tctattctcc gcttcctccc
     2581 agaccacgct ttgcgggccg tcgatcacgt cgccagcgac ttcctccccg cgatgggcgg
     2641 gcaggcagtg catgaacagg gcatcggggc tggcaagccg catcatttcc gcatccactt
     2701 gccaatcctc gaaatcgcgc ttgcgctctt cgttctccgc ctcgaagccc atgctggtcc
     2761 acacgtcggt ggtcaccagg tcggcgccgc gcgccgcttc catgggatcg tcgaactcct
     2821 cgtagtgatc ggtgccatag agcccggcgc gctccggctc gacctcgtag ccgggcgggg
     2881 tggaaacgtg tacgttgaag cccaatacct cggcggcctg cagccaggtg ttgcagacat
     2941 tgttcgagtc cccgatccac gcgacggtct tgccgcgcac cggcccgcgc cgctcgatga
     3001 aggtgtagat gtccgcaagg atctggcagg ggtggtattc gttggtcagg ccgttgatca
     3061 ccggcacgcg cgagtgcgcg gcgaaacgtt cgatgatctc ctgctcgaag gtacggacca
     3121 tcacgatgtc gcacatgcgc gagatcacct gtgccgcatc ttccacaggt tcgccgcggc
     3181 cgagctgcga atcgcgggtg ctgaggaaga tcgccgagcc gccgagctgg tgcatgccgg
     3241 cctcgaaaga cagccgcgtg cgcgtgctct gcttctcgaa aatcatcacc agcgtgcgat
     3301 cgaaaagcgg gttgtgcggc tggtaggcct tgaactgctg cttgatccag cgcgtgcgcc
     3361 cgaacaggta ttc
//
LOCUS       DASQOP010000175        24831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1880507, whole genome shotgun sequence.
ACCESSION   DASQOP010000175 DASQOP010000000
VERSION     DASQOP010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 24831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24831
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1880507"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VE030_07035"
     CDS             complement(<1..602)
                     /locus_tag="VE030_07035"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23202.1"
                     /translation="MKEQTMNMNDKAVREMSSHPRWVSSLYEFVAPHWDNLVNGQWAQ
                     SVATARLSVEQMQGWILQIYPFIYDFPKFLAEGLIKVEDDFSRTFLIENIRIERAHAE
                     HWIWMGEGFGLHRQQMLNLAEGDTPILRDVQSLTDWVWRVNTKGSLAEAVAATSFAIE
                     GVAGALARKIATGFESYRDRPGVDMNPKTYKWIREHCHYD"
     gene            complement(599..2833)
                     /locus_tag="VE030_07040"
     CDS             complement(599..2833)
                     /locus_tag="VE030_07040"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZF23203.1"
                     /translation="MLEETSVRLLYREKFWGTDTAMEYVDIEAGMTAFTRVIQWFAKS
                     KRMLLIVSPFLAIVAVLVWLAIVSMDILAAGRAYVEGESLWSKNQKEAVFHLMRFATT
                     GSESDFRGYRKAIMVPMGFRMARVELEKPHPDDPTVRAGFIQGGTHPDDIPGVISLYR
                     RFHRVSYMKNVLDIWKTGDEFMERLDAAAEELHALQVAGGVDFTGRDTVLNRIIQINE
                     ELRPWQNRFSNTLGEATRWIRNTLLLVILTVAGILIPFGIFLTQRMVSQVDRAERELK
                     ELKLGQEYSKKLAYHASHDPLTNLVNRLEFEKRLALALHTAATQSRQHIVMYLDLDQF
                     KIVNDTCGHAAGDQLLRQVGALLKDQVREGDTLARLGGDEFGVLLENCPMQEAIGIAD
                     KLRQCIADFRFVGEGKAFSIGASIGVVQVADGMLNLTDILSAADAACYTAKEKGRNRV
                     QFYRPHDSEVSMRRGELEWVPRLQGALEQGRFVLYAQQIMSIGAHSPQKSHHEILVRM
                     LDEHGKVVPPMAFIPAAERYSIMPMVDQWVVQNAFAAISSLGPDCGTYAINISGTSIG
                     DERFLEFVREQFRRYVMPPRSVCFEITETAAIANFDKAARFFGEMKSLGCLFSLDDFG
                     AGMSSFGYLKHLPVDFIKIDGSFVKDVAHDPVAVAMVRAINDVGHVMGKKTIGEFVDG
                     EIVLNVLREIGVDFAQGHWISPPSPLAAKPPVAELPVRTGTESAPLPQARTAVREIIL
                     NSTI"
     gene            complement(3159..3944)
                     /locus_tag="VE030_07045"
     CDS             complement(3159..3944)
                     /locus_tag="VE030_07045"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZF23204.1"
                     /translation="MSAVVLLSVERGVATILLNRPQVLNAMDGEMMQQLRPIAESVQN
                     DPAVRAVVVRGEGPAFMAGGDVSVFHSHLHELPELIVHWGREMHFAFLALRRMGKPVL
                     ASVHGAVAGAGFSLMCAADLAIAAADTKFTLAYANIGASPDGGSTHFLPRLLGYRKAM
                     ELALLPERFDAETARLLGLVNWVVAGDKLAEETAKIAQRLASGPTKAYAEAKRLINQS
                     FATTIETQMEEELQAFSRCARGADLKEGVTAFVEKRKPVFKGV"
     gene            complement(3941..6439)
                     /locus_tag="VE030_07050"
     CDS             complement(3941..6439)
                     /locus_tag="VE030_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZF23205.1"
                     /translation="MNGLLWFAAAVTVFWALAYWRVAAPAWLIGVGSYLGALTLWSGA
                     DAETKVLLWTIFLVVAGVLTVTSLRKALISDPLLNWFRKALPQVSQTEQEALDAGTVW
                     WDGELFSGNPDWSKLFGTPKPRLTPDEQAFLDGPVEQLCRICDDWKITHELNDLPPEV
                     WQFIKDKGFLGMIIPRQYGGLGFSAYAHSQVVQKLATRNATACVSVMVPNSLGPAELL
                     LHYGTEEQKNHYLPRLAKGLEMPCFALTGPDAGSDAGSIPDFGLVCKGVHEGREVLGI
                     RLTWEKRYITLGPVATILGLAFKLHDPDHLLGGEEDIGITLALIPTSHPGVNIGRRHL
                     PLNASFMNGPNWGKDVFIPMSWVIGGKEYAGQGWRMLMECLAAGRSISLPSSSAGAAK
                     MAARTTGAYSRVRSQFKIPIGKFEGIEEALARIGGNTYAIDAARTMTAGAVDLGEKPS
                     VISAIVKYHCTERGRQVINDAMDVHGGKGICLGPNNYLGRAYQQIPIGITVEGANILT
                     RSMMIFGQGAIRCHPYVLKEIAATRTQNPAEASGKFDDAFWDHVRFVLANAARALWLS
                     LTGARLVAAVPGAPELRRHVQQLTRLAAGFAFVADVAMLFLGGDLKRKEKLSGRLGDI
                     LSQLYIASTVLKRFEDDGRPQADLPLAQWALEDCLNRIQDAFYGVFGNFPSRIAAWKM
                     RFLVFPWGKVFSPPSDRRGHEVARLLLEPSQVRDRLTANMFLHKHEDDPVGRLELAMQ
                     AAPAGEAVEAKIRNAAKAGVISGLTEESRTASALEKGIIGAEEAAQYRRFSALRRACI
                     MVDDFPHDVGRMQSAREPANVAEMGSVVSQKTAA"
     gene            complement(6436..6825)
                     /locus_tag="VE030_07055"
     CDS             complement(6436..6825)
                     /locus_tag="VE030_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZF23206.1"
                     /translation="MERGYAATSPRMITAKAKVHLAAVNYHFDSKDALIREVFERRLG
                     PLNSARIAHLDGFESGARGRPLTTGRIMEAIVTPALQVNKDPLGAGAAAAGRAQARGV
                     AAQDRQCFRPQGCLKTIHGFAILGARK"
     gene            complement(6942..8735)
                     /locus_tag="VE030_07060"
     CDS             complement(6942..8735)
                     /locus_tag="VE030_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZF23207.1"
                     /translation="MSTYAAPIRDMKFVINELVGLDQISALPGCEEVTPDLVDAVLEE
                     AGKFAAGVLDPLNKLGDRTGARLDNNAVTAAPGFKEAFRQFSEGGWTGLNCDPQFGGQ
                     GLPHIISAQTSEMWNSANMSFCLCPMLTAGVVAALMRHGSDHQKALYLPNLVSGKWTG
                     TMNLTEPQAGSDLAAVRTRAVPEGDHYRLHGTKIFITWGEHDLAENIIHMVLARTPDA
                     PEGVKGISLFVVPKFLVNKDGSLGKRNDVKCVSIEHKLGIHGSPTCVMAYGDTDGAIG
                     HLVGEENRGLEFMFTMMNFARLEVGIEGVAIAERAYQHALEYARTRVQGRETGVKGGD
                     RVTIIHHPDVRRMLMSMKAQTEAMRAIATLASAYLDAALLHPDKTERARNQALFDLLT
                     PVVKGWCTEQSIEIASIGVQVHGGMGFVEEAGAAQYLRDARITTIYEGTTGIQANDLI
                     GRKLAYEKGATIKLMIAEMRALDGELAKVTHPGIAVIRRSLSDGVAALTEASGWLLAT
                     FPHNMKAAAAGAVPFLKLFGVVAGGWQMARAALIAKRRLDEGAEDYDFYRAKIGTARF
                     YAEHVLPLAQAYKQEIVNGSTSVLALEEAQF"
     gene            complement(9018..9947)
                     /locus_tag="VE030_07065"
     CDS             complement(9018..9947)
                     /locus_tag="VE030_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZF23208.1"
                     /translation="MRTLVVAEHDHAALKPAVLNTITAAQRIGGEIDILVAGHNCAEA
                     AKSAAQIAGVKKVLVADGVQYAHPLAENIAALIVSIAKAYSHVLAPATSFGKNVMPRV
                     AALLDVAQVSDIVKVETPDTFVRPIYAGNALATVQSGDAVKVITVRSTGFDAAAATGG
                     SAAVEQLATVADTGLARMVGQELTKSARPELTAARVIVSGGRGMGSGENFRILEALAD
                     KLGAAVGASRAAVDSGFVPNDYQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKVIV
                     AINKDAEAPIFQVADYGLVADLFQAVPELTSQL"
     gene            complement(9947..10696)
                     /locus_tag="VE030_07070"
     CDS             complement(9947..10696)
                     /locus_tag="VE030_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HZF23209.1"
                     /translation="MKVLVPVKRVVDFNVKVRVRADGTGVETANVKMSMNPFDEIAVE
                     EAVRLKEAGAATEIIVISVGSAQCQETLRTALAIGADRAILVETAAETQPLAVAKILK
                     AIVAREQPGLVIMGKQAIDDDSNQTGQMLAALLNWSQATFASKVKVSGNALEVTREVD
                     GGLETLSVNLPAVVTADLRLNEPRYVTLPNIMKAKKKPFEALTPDALGVDVAPRLKTL
                     KVQEPPKRNAGQMVKSVPELVQKLKNEAKVI"
     gene            complement(10959..12590)
                     /locus_tag="VE030_07075"
     CDS             complement(10959..12590)
                     /locus_tag="VE030_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HZF23210.1"
                     /translation="MSHVNPYRTGLDRNPANHVALSPLSFLARAAEVYPQRTAVIHGE
                     RRLTWAQTYQRCRRLASALARHGVGVGDTVAVMAPNVPAMFETHYGVPMTGAILNTLN
                     TRLDAEAIAFMLVHGEARVLIADREYSSTIARALRQIERKPLVIDIDDVLGAGGELLG
                     EVEYENFIAGGDPDFDWRLPDDEWDAIALSYTSGTTGNPKGVVTHHRGAYLNAISNIV
                     TWAMPHFPVYLWTLPMFHCNGWCFPWTLAALAGTSVCLRRVEAAAIFDLIRRHKVTHF
                     CGAPVMHNMLINAPAELRAGIDHKVSAMVAGAAPPQAVIEGMERIGVDLTHVYGLTEV
                     YGPASVCAKHPEWDALPPDQRAELNGRQGVRYVLQEAMDVLDPKTLKPVPRDGKSMGE
                     IFFRGNITMKGYLKNPQATEEAFEGGWFHTGDLAVMYPDGYARIKDRSKDIIISGGEN
                     ISTIEVEDVLYRHPHVLEAAVVARPDEKWGETPCAFVTLKNGAPAVTEQDIIAFCRER
                     LAGFKCPRKVVFGPLPKTSTGKIQKNVLRDQAKTA"
     gene            12885..14003
                     /locus_tag="VE030_07080"
     CDS             12885..14003
                     /locus_tag="VE030_07080"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZF23211.1"
                     /translation="MMPERANRTFICSVLFLDIAEYSRKPVSEQIQLKDQFNALIAEA
                     IRDIAPNDRIILDTGDGVSINFLGDPEDALFVAMSLRESFAPRPNEPPKLPARIGVNL
                     GPVRLVRDLNGQPNIIGDGINVAQRVMGFALPGQILVSRSYFDMVSHISEGYAKLFRN
                     EGSHTDKHVREHEIYSVGYSNADDTTIRSPAERAQVSSREGIRQQATATIATGTGGWL
                     AVIEPWLNNRTIAYGTATLSVGAFALAVLLNFLGQSNLPAAPVKHEAQTAQKKSHAAG
                     KAEEPAVSGFPGPTEKKPAAREAAKPAGTGVIALAIAPWGEVYVDGDRVGVSPPVNEV
                     EVAPGRRKIEIRNGRFPVYTQVVDVKADQKVRIKHKFN"
     gene            14018..14407
                     /locus_tag="VE030_07085"
     CDS             14018..14407
                     /locus_tag="VE030_07085"
                     /inference="COORDINATES: protein motif:HMM:NF038027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TssQ family T6SS-associated lipoprotein"
                     /protein_id="HZF23212.1"
                     /translation="MPTIRLALILILAANLAGGCATGSFRDLFHARTAGSNYLEEGVR
                     NYEEGNYRTSARRIQYALEEGLSRPDRVKAHKYLAFIACVSGQQLTCREEFSIALELD
                     PSFELDAAEAGHPIWGPVYKSVKAKQK"
     gene            14421..15758
                     /locus_tag="VE030_07090"
     CDS             14421..15758
                     /locus_tag="VE030_07090"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZF23213.1"
                     /translation="MSPNSSIATLGRYEILAELGQGAMGVVYKARDPMLDRVVAIKTV
                     NLTLPKDELAEYEARFYQEAKAAGGLSHRNIVTIYDIGRSERIAYMAMELLEGDELRK
                     LLQARAPIPLSRALDIAAQVAEGLQYAHERQIIHRDIKPANIMVLQDGLVKITDFGIA
                     RMRNNEVKTMTGMILGSPKYMSPEQVSGKRADTRSDIFSLGVVIYEMLTGTSPFVADN
                     IHGVMYQTMNFNPPAPKTLNPDLPEVLNFIIAKALAKNLDDRYQKANDLAHDLREAMA
                     TLIGASATPEVVSGPVEGGPFVLPESALTRQQDRAARILGTAGAQQEQQSGEEQPDPS
                     ASETTQPALGLSKAFDSFDATMRLAALTGMEKELDEFSETQKIARLKALSQAKRAAAP
                     ATSPLSASMTWQRSGSLIRSVGSGGQSASANLKVVWIGSALLALAAAALFLLH"
     gene            complement(15898..17247)
                     /gene="radA"
                     /locus_tag="VE030_07095"
     CDS             complement(15898..17247)
                     /gene="radA"
                     /locus_tag="VE030_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006754543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HZF23214.1"
                     /translation="MAKAKTVYACTECGGQTLKWQGQCPHCSAWNTLVETIAEKASSR
                     FQALAQSSKLQKLADIEAQEEPRRPTGIGELDRVLGGGLVHGAVVLLGGDPGIGKSTL
                     LLQALSLMGGSCNAVYVSGEESVQQIALRAKRLAGGAETLNLLAEIQLEKVLSVLAAQ
                     KPDVAVIDSIQTIYSEALQSAPGSVAQVRECAAQLTRYAKTSGATLLLVGHVTKEGAL
                     AGPRVLEHMVDAVLYFEGDAHSAFRLVRATKNRFGAVNELGVFAMTDRGLRGVSNPSA
                     LFLSQHGTPVAGSCVMVTQEGTRPLLVEVQALVDEAHAPNPRRLSVGLEQNRLAMLLA
                     VLHRHAGIAAFDQDVFVNAVGGVKIDEPAADLPVLLAIVSSLRNKPLPEKLVAFGEIG
                     LAGEVRPVQRGQERLREAAKLGFRTAIIPKANAPRTAIEGIEVVTVDRVEEAVARSR"
     gene            complement(17252..18328)
                     /gene="alr"
                     /locus_tag="VE030_07100"
     CDS             complement(17252..18328)
                     /gene="alr"
                     /locus_tag="VE030_07100"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HZF23215.1"
                     /translation="MARPIQASFDLAALRSNFAIARRLAGVAKVMAVVKANAYGHGLL
                     RVTRALADADGFALLELDDAVRLRDAGYRQRILLLEGFFRADELPVILHQRFSVVIHN
                     DEQMAMLGKLHGDGKLEVFVKFNTGMNRLGFPAAGAGRVLEQLKTLPCVGEVVLTTHF
                     SDAEGVSGIEWQMREFERHVARADYARSLANSAALLRFPEARGEWVRPGIMLYGASPL
                     EDRRAAQIGLRPAMSLTSEIIGIQNLKRGDTVGYGSLFTAHRETRIGVVACGYADGYP
                     RHAYNGTPVVVDGVRVPMVGRVSMDMLCVDLSDIPRAGIGSPVTLWGAGLPVEEVAAA
                     AGTVSYELLCALARRVPVEEHRGD"
     gene            18529..19761
                     /gene="lplT"
                     /locus_tag="VE030_07105"
     CDS             18529..19761
                     /gene="lplT"
                     /locus_tag="VE030_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HZF23216.1"
                     /translation="MNRGFYTILAAQFFSALADNALLFAAIALLASLHAPAWQTPVLQ
                     QFFVFAYIVLAPFVGPFADSLPKGRVMFVANAVKLAGCLAMLLGMHPLFAYGIVGVGA
                     AMYSPAKYGILTEYLPHQRLVFANGWMEGLTVAAIILGAILGGIMLGPEFADYVLKHG
                     ELPGVDLWIDTAPEVAIVCILTLYFVAAIINLYIPKVAIDHRLPKRNPLFILQDFWHS
                     LSLLWRDPLGQVSLAVTTLFWGAGATLRLIVLTWAALALKFDLKRATQLTAVVAIGIA
                     LGSILAAKYVSLQRAVRVLPVGIAMGLVVVSMMFVTDWRVAIGLLVLVGAMGGYFVVP
                     MNALLQHRGHLLMGAGHSIAVQNFNENLSILLLLGIYALMIKSELSMNSMIVAFGLFI
                     TTTMAWITIKHWHDQDPQ"
     gene            complement(19777..20334)
                     /locus_tag="VE030_07110"
     CDS             complement(19777..20334)
                     /locus_tag="VE030_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HZF23217.1"
                     /translation="MIQHLVEHYGYLAVFVGALLEGETVLVMGGFAAHRGLLELPWVL
                     ALAAVGGFLGDQFFFFMGRRHGRAMLSRFPSLVQSSTRVQSLLTRYDAAAIFSVRFIY
                     GLRIAGPVLIGMSGVRPWRFVCFNLLGAGAWAAAIGGAGYLFGTAMEAFLSDLRRYEA
                     VILILIAVCGGALWLWALLRKRRSR"
     gene            complement(20350..20847)
                     /locus_tag="VE030_07115"
     CDS             complement(20350..20847)
                     /locus_tag="VE030_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HZF23218.1"
                     /translation="MKLARMLRHLTMPHWRARQLLPRGALLRIEHAIGESESRRSGQI
                     RFAVENALDLHALLRGQSARERAIEVFAALRVWDTEHNDGVLIYLLLADRDVEIVADR
                     GIHPKVGSQGWEDICREMEAAFRVGRFEYGVLDGIERVARHLQTHFPRNVPGPNELPD
                     RPVVL"
     gene            complement(20849..21700)
                     /locus_tag="VE030_07120"
     CDS             complement(20849..21700)
                     /locus_tag="VE030_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HZF23219.1"
                     /translation="MMLLMRAVAAAALLLAFCAVAEDASIPPLTGRVVDLNSTLSAEQ
                     KRELEASLEAFEKRKGSQIAVLITGTTFPEPIESFAMRVAEAWKIGRKGVDDGIVVVI
                     AKSDQAMRIEVGYGLEGAVPDAVAKRLIEEEFIPKFREGDFYGGLRAGLDRLVRVIDG
                     EPLPAPKQEAGRGSEPRSIEAYFVLFMAIVIAVGGLLRTLFGRLPAATIVGAGTGLLA
                     WLIVAPVVVALLVGIVGFIFTLAGGGHRGGWGGGVGGGFGGGGFGGGGFSGGGGGFGG
                     GGASGRW"
     gene            complement(21705..22319)
                     /locus_tag="VE030_07125"
     CDS             complement(21705..22319)
                     /locus_tag="VE030_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HZF23220.1"
                     /translation="MKRFLALPFLLVISFGLAGCGYNTLQTRDEQIKAAWAEVLNQYQ
                     RRADLVPNLVNTVKGFAAQEKDVLLGVTNARARVGSMQATPELVNDPEAFSKFIAAQG
                     AMTSALSRLLLVAENYPQLKSDANFRDLQAQLEGTENRIAVARQRYIRAVQDYNVTVR
                     SFPSNLTAMLFGYKVKPTFTVENEQVISKPPTVDFSKPEPAQNK"
     gene            complement(22349..22924)
                     /locus_tag="VE030_07130"
                     /pseudo
     CDS             complement(22349..22924)
                     /locus_tag="VE030_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011851828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
     gene            complement(23101..23562)
                     /gene="rimI"
                     /locus_tag="VE030_07135"
     CDS             complement(23101..23562)
                     /gene="rimI"
                     /locus_tag="VE030_07135"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HZF23221.1"
                     /translation="MSAQLDSLPKLRPMRASDLDRIMEIEPVIYTHPWTRGNFDDSIR
                     VGYSCWVIDCSAVVVGYGVLMIGVREAHLLNLSVAPNWQGRGLGRSLLEHFVRIGRDS
                     DAQQMFLEVRPSNASARRLYADFGFRDISVRRGYYPVAGGREDAILMGLML"
     gene            complement(23559..24233)
                     /gene="tsaB"
                     /locus_tag="VE030_07140"
     CDS             complement(23559..24233)
                     /gene="tsaB"
                     /locus_tag="VE030_07140"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HZF23222.1"
                     /translation="MNILALDTSTEYCSVALLHDGKLTFREELAGQTHSELILAMIDA
                     LLRTSNLGLAHLHGIAFGAGPGSFTGLRIACGVAQGLAFGAGLPVVPVGTLLALAEAA
                     GAPRVVACLDARMGEIYHAAYQRNGAGWSETVMPNVCKADAAPALEGRDWVGCGSAFA
                     VYGDALAARYHGQLARVQSQAFPHAREVAQLALPVLAAGHGVPAEQAVPLYVRDKVAR
                     KMHEQP"
BASE COUNT         4729 a         8066 c         7335 g         4701 t
ORIGIN      
        1 tcgtcgtagt gacaatgctc ccgaatccac ttataggtct tcggattcat gtccacgccc
       61 ggacgatccc ggtaggactc gaagcccgtg gcaatttttc tcgcgagcgc acccgcgacg
      121 ccttcgatgg cgaagctcgt cgcggccacc gcttcggcaa gagaaccttt ggtgtttact
      181 cgccacaccc agtcggtaag cgactgcacg tcccgcagga tgggtgtatc gccctcggcc
      241 agattcaaca tttgctggcg atgcaggccg aatccctcgc ccatccatat ccaatgttcc
      301 gcgtgcgctc tttcaatacg gatgttttca atcagaaatg tccgggaaaa gtcgtcctct
      361 accttgatca agccctcggc gagaaacttc gggaaatcat agatgaacgg gtagatctga
      421 agaatccatc cttgcatctg ttcgacactg agacgcgccg tagcgaccga ctgcgcccat
      481 tgcccgttga ccagattatc ccagtggggg gccacgaact cgtataacga agaaacccac
      541 cgggggtgcg aagacatttc ccgaaccgcc ttgtcattca tgttcatggt ctgctctttc
      601 atattgtgga gttaagaatt atttcccgga cagcagtccg cgcctgcggc agaggagcgg
      661 attctgtccc ggtacgcact ggaagctctg caacgggggg ctttgctgct aacggactgg
      721 gcggagatat ccagtgcccc tgcgcgaagt ccactccgat ctcgcgtaat acattgagta
      781 cgatctctcc gtccacaaac tcgccgattg ttttcttgcc catcacatgc cccacgtcgt
      841 tgatggcccg caccatcgcg acggcaaccg ggtcatgcgc tacgtccttg acgaaactgc
      901 cgtcgatctt gatgaagtcc acgggtagat gcttgaggta tccgaaagag gacattccgg
      961 ccccgaaatc gtccaatgag aacaggcagc caagcgattt catttcgcca aagaaccgcg
     1021 ccgccttgtc aaagttggca atcgcggcgg tttccgtgat ctcgaaacaa acgctccgcg
     1081 ggggcatgac atatctccgg aactgttctc ggacgaattc aaggaagcgt tcgtccccga
     1141 tcgatgtgcc cgaaatattg attgcatagg ttccgcaatc cgggcccaag gaactgatcg
     1201 ccgcgaacgc gttttgaaca acccactggt ctaccatcgg catgatgctg tagcgttccg
     1261 cggcagggat gaacgccatc gggggaacga ccttgccatg ctcgtcgagc atgcgcacga
     1321 gaatctcgtg atgactcttc tgaggagagt gcgccccgat cgacatgatc tgctgagcgt
     1381 aaagcacgaa gcggccttgc tcgagcgccc cttgcagtcg gggcacccat tccagctcgc
     1441 cacggcgcat ggaaacctca ctgtcgtgcg gtcgatagaa ctgaacgcga ttgcgcccct
     1501 tttccttcgc cgtgtagcag gcggcatcgg cggcgctcag tatgtcggta aggttgagca
     1561 tcccatccgc gacctggacc acgccaatgc ttgcgccgat gctgaacgct ttgccttcgc
     1621 cgacaaagcg aaaatcggct atgcactggc gcagcttgtc ggcgatgcca atcgcctcct
     1681 gcattggaca gttttcaagc aatacaccga actcgtcccc acccagacgc gcaagagtat
     1741 cgccttcgcg cacctggtcc tttagtaatg cacccacctg ccgcaataat tgatccccgg
     1801 ccgcgtgccc gcaggtatcg ttgacgattt tgaactgatc gaggtccagg tacatgacga
     1861 tgtgctgccg cgactgagtg gccgcggtgt gtaaagccag tgccaggcgc ttttcgaatt
     1921 caaggcggtt aaccagattg gtgagtgggt cgtggctggc gtggtaggca agcttcttgg
     1981 agtattcctg ccccagcttg agttccttga gttcgcgttc cgcgcgatcg acttgactga
     2041 ccatgcgctg cgtaagaaaa ataccgaacg gaatcagaat tcccgctacc gtgagaatca
     2101 ccagcaacaa cgtgttgcgg atccagcgcg tggcctcacc caatgtgttt gaaaaccggt
     2161 tctgccacgg cctgagttct tcgttgatct ggatgatgcg gttcaatacg gtatcgcgcc
     2221 cagtgaagtc cactccaccg gccacctgca atgcatggag ttcttcagcc gccgcatcaa
     2281 gtctctccat gaactcgtct ccggttttcc agatatcgag cacgttcttc atgtagctca
     2341 cacggtgaaa gcgcctgtac aggctaatga cacccgggat gtcgtccggg tgggttccgc
     2401 cctggatgaa tccggcccgc acggtggggt catccggatg gggcttttca agttcaacgc
     2461 gagccatacg gaaacccatc ggcaccatga tcgctttgcg atagccgcgg aaatccgatt
     2521 cggagccagt cgtggcaaaa cgcattaagt ggaataccgc ttccttctgg ttcttcgacc
     2581 acaggctctc gccttcgacg tatgcgcgac ccgcggccag gatgtccatg ctgacaatgg
     2641 cgagccagac cagtactgcg acgatcgcca ggaacggcga aacgataagc agcatgcgtt
     2701 tgctctttgc gaaccactga atgactctcg taaacgcggt catccctgcc tcgatatcaa
     2761 cgtattccat cgctgtgtcc gtcccccaga atttttcgcg gtaaagtaag cgaacgctgg
     2821 tttcctcgag caaaccgcgc cccctaacgt taagagcagg ttgcatgcct gaaagcggga
     2881 acgaacgggt cgtgaagcca gaaaacgttg caattcaggg gcaaagtccg aatggatcct
     2941 tgaataggca tgtcgccagg cagcgaccac ccggccggaa acgcgtagcc tccgacactt
     3001 agcgacagga acaaggcggg cgtgtggaaa tttcgacagc cactggctgc cgaacttatt
     3061 ggactaagtc tagattatta ccattgacca ggctggaatt ctccgcttta cgcccgttag
     3121 tttttgattc ccttcgtatc ctgggcggag attgcgtttc aaacgccctt gaaaacgggt
     3181 tttcgttttt caacgaatgc ggtcacacct tccttcaaat cggcaccccg cgcgcaccgg
     3241 gaaaatgcct gcaattcctc ctccatctgc gtctcgatcg ttgtggcgaa cgactggttg
     3301 atcagccgct tggcctcggc ataggccttg gtcggcccgc tggccaggcg ctgcgcgatc
     3361 ttcgcagttt cttcggcaag tttgtcgccg gcaaccaccc agttcaccaa acctagcaac
     3421 cgcgccgttt ccgcatcgaa gcgctccggc agtaatgcca gttccatcgc cttgcggtag
     3481 cccagcaggc gcggcaggaa atgcgtgctg ccgccgtccg ggctggcgcc gatgttggcg
     3541 taggcaagcg tgaatttggt gtccgccgcc gcaatcgcca ggtccgcggc gcacatcagg
     3601 ctgaatccgg cgccggcaac ggcgccgtgc acgctggcca gaaccggctt gcccatccgc
     3661 cgcagcgcca gaaacgcgaa atgcatctcc cgcccccaat gcacgatcag ctcagggagt
     3721 tcatgcagat gagaatggaa taccgacacg tcgccgccgg ccatgaaggc ggggccttct
     3781 ccgcgcacga ctacggcccg caccgccgga tcattctgca ccgactccgc aatcggcctc
     3841 agttgctgca tcatctcgcc gtccatcgcg ttcagcacct gcggacgatt gagcaagata
     3901 gtggctacac cccgctccac cgacagcagg acgaccgcgc tcatgccgcg gtcttctgcg
     3961 aaacgacgct acccatttcc gcgacgttcg ccggttcgcg tgccgactgc atgcgcccga
     4021 cgtcatgcgg gaagtcgtcg accatgatgc aggcgcgccg cagcgcgctg aagcgccggt
     4081 attgtgcggc ttcctccgcg ccgatgatgc ccttctccag cgccgatgcc gtacgcgact
     4141 cctcggttaa gccggagatc acgccggcct tcgccgcatt tctgatcttc gcttccaccg
     4201 cctcgccggc tggtgccgcc tgcatcgcca gttcaagccg tcccaccggg tcgtcctcgt
     4261 gcttgtgcag aaacatgttc gcggtcaggc ggtcgcgcac ctgcgaaggc tccagaagca
     4321 gccgtgccac ctcgtgacca cggcgatcgc tgggcgggct gaacaccttg ccccagggga
     4381 agaccaggaa acgcattttc caggccgcga tgcggctcgg gaaattcccg aacacgccgt
     4441 agaacgcatc ctggatgcgg ttgagacaat cttccagcgc ccactgcgcg agcggcagat
     4501 cggcctgcgg ccggccatcg tcctcgaatc gcttcaatac ggtggaagcg atgtagagct
     4561 ggctcagaat gtcgcccaac cgtcccgaca gtttttcctt gcgtttcagg tcgccgccga
     4621 ggaacagcat ggcgacgtcc gccacgaacg caaaaccggc ggccagccgg gtcagttgct
     4681 ggacatggcg gcgcagttcc ggcgcgccgg gtaccgcggc gaccaggcgt gcgccggtca
     4741 ggctcagcca aagcgcccgg gcggcgttgg cgagcacaaa acgcacgtga tcccagaatg
     4801 cgtcatcgaa tttcccgctc gcttccgctg gattttgcgt tcgggtcgcc gcgatttcct
     4861 tgagcacgta aggatggcag cggattgcgc cctgtccgaa gatcatcatg cttcgggtga
     4921 ggatgttggc gccttcgaca gttatgccga tcggaatctg ctggtaggcg cggcccagat
     4981 agttgttggg gccgaggcag atgcctttgc cgccgtgcac gtccatagca tcgttgatga
     5041 cctggcggcc gcgctcggtg cagtgatact tgacgatcgc ggatatgacc gacggtttct
     5101 cgcccaagtc caccgcgccc gcggtcatgg tgcgcgccgc gtcgatggcg taggtattgc
     5161 ccccgatgcg cgccagcgcc tcttcgatac cctcgaactt gccgatggga atcttgaact
     5221 ggctgcgcac ccggctatag gcgccggtgg tgcgtgcggc catcttggct gcgccggcgc
     5281 tcgaggacgg cagcgagatc gagcgcccgg ccgccagaca ctccatcagc atgcgccagc
     5341 cctggccggc gtattccttg cctccgatca cccagctcat gggtatgaat acgtccttgc
     5401 cccagttcgg gccgttcatg aacgaggcat tgagcggcag gtggcggcgg ccgatattca
     5461 cgcccggatg actggtcgga atcaatgcca gcgtaatacc gatgtcttcc tcgccgccca
     5521 gcagatggtc cggatcatgg agcttgaacg ccagaccgag gatcgtcgcc accgggccga
     5581 gcgtgatgta acgcttctcc catgtaaggc gaatgcccag gacttccctg ccttcgtgca
     5641 cgcccttgca gaccaggccg aagtcgggga tcgagccggc gtcggagccg gcgtcgggac
     5701 cggtgagcgc gaagcacggc atctcgagac cctttgccag ccgcggcagg taatgattct
     5761 tctgctcttc ggtaccgtaa tggagcagga gttcggctgg tcccagcgaa ttcggcacca
     5821 tcaccgatac gcatgccgtg gcgttacgcg tggcaagctt ctgtaccacc tgcgaatgcg
     5881 cgtatgcgga aaatcccagg ccgccgtact ggcgcggaat gatcatgccg aggaagccct
     5941 tgtccttgat gaactgccag acttccggcg gcagatcgtt cagctcgtgg gtgatcttcc
     6001 agtcgtcgca gatgcggcac aattgctcca ccggaccgtc gaggaaggcc tgctcgtccg
     6061 gggtcaggcg cggcttcggt gtgccgaaca gcttgctcca gtctggattg ccgctgaaca
     6121 attcgccgtc ccaccacacc gttccagcat cgagcgcctc ctgctcggtc tgtgaaacct
     6181 ggggcagcgc cttgcggaac cagttgagca acggatcgct gatcagcgcc ttgcgcaatg
     6241 acgtcaccgt cagcacgccg gccactacca gaaaaatggt ccacagcagc actttggttt
     6301 cggcatccgc acccgaccag agcgtcaatg cgccgaggta ggaacccaca ccgatcaacc
     6361 aggccggcgc ggcaacgcgc cagtaggcca gcgcccagaa caccgtcacc gcagcggcaa
     6421 accagagtaa tccgttcatt ttctagctcc caaaatcgcg aatccgtgaa tagtcttcag
     6481 gcagccttgc ggccggaaac actgccgatc ttgcgcggcg acgccccgcg cttgtgctct
     6541 gcctgcggca gcggcgcctg cgccgagcgg atctttgttg acctgcaacg ccggggtaac
     6601 gatcgcttcc atgatccgcc cagtggtgag cggcctgccg cgagcgccgg attcaaaacc
     6661 atcgagatgg gcgatgcgtg cggaattcag cggaccgaga cggcgctcga acacttcgcg
     6721 gatcaacgcg tccttcgaat cgaagtgata attgaccgcc gcgagatgga ctttcgcctt
     6781 ggcggtgatc atccgcggcg aggtggcggc ataaccgcgt tccataacaa gtgtttgaat
     6841 gttggcggct gagtccgggc ttgtcaacga aaaccttcag gagaaaagct cacttctcaa
     6901 gtggtgagat tttggtgtat ggattgcggc acgcccgcgg gttagaactg ggcttcctca
     6961 agcgccagaa cgctggtcga gccattcacg atttcctgct tgtaggcctg ggcgagcggc
     7021 aggacatgtt ccgcatagaa acgcgccgtg ccgatcttgg cgcggtaaaa atcgtaatcc
     7081 tcggcacctt catcaagacg gcgcttggcg atcagcgcgg cgcgcgccat ctgccatccg
     7141 cccgcgacga cgccgaacag cttcaggaaa ggcaccgccc cggcggcggc ggctttcatg
     7201 ttgtgtggaa acgtcgccag cagccagccg ctcgcctccg tcaatgccgc cacgccatcg
     7261 gacagcgaac ggcggatgac cgcgatgccc ggatgggtga ctttcgccag ttcgccgtcc
     7321 agcgcacgca tctccgcgat catcagcttg atcgtcgcgc ccttttcgta cgcgagcttg
     7381 cggccgatca ggtcgttcgc ctggatcccg gtggtgccct cgtaaatcgt ggtgatgcgc
     7441 gcgtcgcgta ggtactgcgc cgctccggct tcctccacga aacccatgcc gccgtggacc
     7501 tgcacgccga tggaggcaat ctcgatgctt tgttcggtgc accagccctt gaccaccggg
     7561 gtgagcagat cgaacagcgc ctggttccgc gcgcgttcgg tcttgtccgg gtgcagcagt
     7621 gccgcgtcca gatacgccga agcgagggtc gcgatcgccc gcatcgcctc ggtctgcgcc
     7681 ttcatgctca tcagcatgcg gcgcacgtcg gggtggtgga tgatggtgac gcgatcgccg
     7741 cccttgacgc cggtctcacg gccctgcacg cgggtcctcg cgtactccag cgcgtgctga
     7801 taggcgcgct ccgcaatggc cacgccttcg atgccgacct caagccgcgc gaagttcatc
     7861 atggtgaaca tgaactccag tccgcggttc tcttcaccga ccagatggcc gatcgcgcca
     7921 tccgtgtcgc cgtaagccat cacgcaggtt gggctgccgt ggatgccgag cttgtgctcg
     7981 atggacacgc atttgacatc gttgcgcttg cccaggctgc cgtccttgtt caccagaaac
     8041 ttcggcacca cgaacagcga aatgccctta actccttcgg gcgcgtccgg cgttcgtgcc
     8101 agcaccatgt ggatgatgtt ctccgccaag tcgtgctcgc cccaggtgat gaagatcttg
     8161 gtgccatgga gccggtagtg gtcgccctcg ggaacggcgc gcgtgcgcac cgctgcgaga
     8221 tcggaacccg cctgcggctc ggtgagattc atggtcccgg tccacttgcc tgacaccagg
     8281 ttcggcagat acagcgcctt ctggtggtcc gagccgtggc gcatcagcgc ggcaaccaca
     8341 cccgcggtaa gcatcgggca caggcagaac gacatgttgg ccgagttcca catttcggag
     8401 gtctgcgccg agatgatgtg cggcagcccc tgcccgccaa actgcgggtc gcagttcagt
     8461 ccggtccaac cgccttcgga gaattgccgg aacgcttcct tgaaacccgg cgcggcggtg
     8521 acggcattgt tgtcgagcct ggcgccggtc cggtcgccga gcttgttgag cggatccagt
     8581 acgccggcgg cgaacttgcc ggcttcctcc agcaccgcgt caaccaggtc cggggtaact
     8641 tcctcgcatc cgggcaacgc cgaaatctgg tcgaggccga ccaactcgtt aatgacgaac
     8701 ttcatgtcac ggatcggcgc ggcgtatgtg gacatgtctt actcccgaat cgttgaaacc
     8761 gaagaacctc aagaaaaacc tttcaccacg gaggacacgg aggaaagact aaagaggaaa
     8821 aagcaatcga gagttaacag cgggaccgat ggcgaattac cgttactgcg aagttcgatc
     8881 gttcgcacgc tgtcaagagc gaaaaactgg tgcggcgcta ccgtcaccac aggccacggt
     8941 tttcactcca gtttgtcgtt tcttttcctc tgtgtcctcc gtgtcctccg tggtccagga
     9001 tctttgttcg cagttgatca gagctggctc gtcaactcgg gcacggcctg gaacaagtcg
     9061 gcgaccagcc cgtagtcggc cacctggaag atcggcgctt ccgcgtcctt attgatcgcc
     9121 acaatcacct tgctgtcctt cattccagcg aggtgctgga tcgcaccgga gatcccgacc
     9181 gcgatgtaga gctcgggcgc gacgatcttg ccggtctggc cgacctgata gtcgttcggc
     9241 acgaaacccg agtccactgc cgcacgcgaa gcgccgactg ccgcgccgag cttgtccgcc
     9301 agagcttcca gaatcctgaa attctcgccg ctgcccatgc cgcggccgcc ggagacgatc
     9361 acgcgcgccg ccgtcaactc cggccgcgcc gacttggtaa gttcctggcc taccatcctg
     9421 gcaagcccgg tgtcggcgac agtcgctagc tgttcaaccg cagccgaacc gccggtcgcg
     9481 gcggcggcgt cgaaaccggt cgagcgaacg gtgatcactt tgaccgcgtc gccggactgg
     9541 acggtcgcga gcgcgttgcc cgcgtaaatc ggccgcacga acgtatcggg cgtttcgacc
     9601 ttgacgatgt cggaaacctg cgccacatcg agcagcgccg ccacgcgcgg cattacgttc
     9661 ttgccgaaac tggtggccgg cgccagcacg tgggagtaag ccttcgcgat cgagacgatc
     9721 agcgccgcga tattctccgc cagcggatgc gcgtactgca cgccatccgc gaccagcact
     9781 tttttcactc cggcaatctg cgccgcggac tttgcagctt cagcgcagtt gtgcccggca
     9841 accaggatgt cgatttcgcc gccgattctc tgcgccgcgg ttatggtatt gagtaccgcg
     9901 ggcttcaacg ccgcgtggtc atgttcggca acgaccagag tacgcatcag atgaccttcg
     9961 cttcattctt cagcttttgc acgagttccg gcacgctctt caccatctgg ccggcgttgc
    10021 gcttcggcgg ttcctgcacc ttgagcgtct tcagccgcgg cgcgacgtcc acgccgagcg
    10081 catccggcgt cagggcctcg aacggctttt tcttcgcttt catgatattc ggcagcgtaa
    10141 cgtagcgcgg ctcgttcaag cgcaggtcgg cggtcaccac cgccggcaga ttgacggata
    10201 gcgtctccag gccgccgtct acttcacggg tcacttccag ggcgttgccg ctcaccttca
    10261 ctttcgaggc gaaagtggcc tgtgaccagt tgagcagcgc cgccagcatc tgtccggtct
    10321 ggttagaatc gtcgtcgatc gcctgcttgc ccatgatgac cagtcccggc tgctctctgg
    10381 cgacgatcgc cttgagaatt ttggctacgg ccagcggttg cgtttccgcg gcggtttcca
    10441 ccagaattgc gcggtcggcg ccgattgcca gcgcagtacg cagcgtttcc tggcattggg
    10501 cagagccgac ggagatcacg atgatctcgg tcgcggcgcc cgcctccttc agtcgcaccg
    10561 cttcttcgac ggcaatctcg tcgaatggat tcatcgacat tttcacgttg gcggtctcga
    10621 ccccggtgcc gtcagccctc acccgcacct tgacattgaa gtccactacc cgctttaccg
    10681 gaaccagtac cttcatttca tggaccttcg cttgcgtgaa agggatgaga ttctaccgca
    10741 gcaacgtcaa ataccttcac cgtcaacaac gcccaccact agaacatttt caccgcaaag
    10801 acgcaaagga cgcgaagaaa tacaaaacca gcatgctggc cgcctcaaaa ccagagcgcc
    10861 cggcacttca gatcacgagt gattccgcct gtcgatccac taaattgcgt ttttcctgtt
    10921 cttctctgcg ttctttgcgt ctctgcggtg aaagggggtc acgcggtttt cgcctggtca
    10981 cgcagaacgt ttttctggat cttgccggtg gaggtcttgg gcagcgggcc gaacaccacc
    11041 ttgcgcgggc acttgaagcc cgccagccgc tcgcggcaga aggcgatgat gtcctgctcg
    11101 gtgaccgccg gcgcgccatt cttgagcgtc acgaacgcgc acggtgtctc cccccatttc
    11161 tcatccggcc tcgctaccac cgccgcttcc agcacgtgcg ggtgccggta gagcacatcc
    11221 tccacttcga tggtggaaat gttttctccg ccggagatga tgatgtcctt cgaccggtcc
    11281 ttgatcctgg cgtagccatc cgggtacatc accgccagat cgccggtgtg aaaccatccg
    11341 ccctcgaaag cctcttcggt ggcctgcgga ttcttgaggt agcccttcat agtgatgttg
    11401 ccgcgaaaga agatttcgcc catggacttt ccgtcgcgcg gcaccggctt cagcgtcttg
    11461 ggatcgagca cgtccattgc ctcctgcagg acgtaacgca caccctgccg tccgttcagt
    11521 tctgcgcgct ggtccggcgg cagcgcatcc cactccggat gcttggcgca caccgatgcc
    11581 gggccgtaga cctcggtcag accatagaca tgagtcagat ccacgccgat gcgctccatg
    11641 ccttcgatga ccgcctgcgg cggcgcggca cccgccacca tcgccgaaac cttgtgatcg
    11701 attccggcgc gcagttcggc cggcgcattg attagcatgt tatgcatcac cggcgcgccg
    11761 cagaaatgcg tgaccttgtg gcgtcggatc aggtcgaaaa ttgctgcggc ttcgacccgt
    11821 cgcaggcaca cgctggtgcc ggccagcgcg gccaatgtcc acgggaaaca ccaaccgttg
    11881 caatggaaca tcggcagcgt ccacaggtac accgggaagt gcggcatggc ccaggtgacg
    11941 atgttgctga tcgcattgag ataggcgccg cggtgatgcg tcacgacgcc cttgggattt
    12001 ccagtcgtgc ccgaggtgta ggacagcgca atcgcgtccc actcatcgtc cggaagcctc
    12061 cagtcgaagt ccggatcgcc gccagcaatg aaattttcgt attcgacctc gcccagcagt
    12121 tcgccgcccg cgccaagcac atcgtcgatg tcgatgacca gcggcttgcg ctcgatctgc
    12181 cgcagcgccc tggcaatcgt cgaggaatac tcgcggtcgg cgatgagcac cctggcctcg
    12241 ccgtgaacaa gcatgaacgc gatcgcctcg gcatccaggc gcgtgttgag cgtattcaga
    12301 atcgcacccg tcatcggcac accgtagtgc gtctcgaaca tcgccggcac gttgggcgcc
    12361 atgacggcga ccgtgtctcc aacaccgacg ccgtgcctgg cgagcgccga ggccagcctg
    12421 cggcagcgtt ggtaggtttg cgcccaggtc aggcgccgct cgccgtgaat caccgcggtg
    12481 cgctgcggat agacctccgc ggcgcgcgcc agaaacgaca gcggtgacag agcgacgtgg
    12541 ttggccggat ttcggtccag gccggtgcgg taaggattga cgtgactcaa agctgctccg
    12601 gaaatctgca aaattcaggg cgccggggtt tgttgccgcg tccagccccg gcgctccggc
    12661 gggacaccca ataataaggg attgcgtcga atccacggcc gcaggaggtg cctgcaaaac
    12721 ctcgaaaacc gtggaagatt gttcgcaaag agcttgactc aagtgaataa atttattccg
    12781 gacgcttgct ggaccgacac tgaatgatga taagattaac gccccccgga agcgtgtgcc
    12841 cgccaaggaa ggccgtttga agccgacaac gcaggtgatg ttcgatgatg cctgagcgag
    12901 ccaaccgcac attcatttgc agtgtgctgt tcctggacat cgccgaatac tcgaggaagc
    12961 cggtgtcgga gcagatacag ctcaaggatc agttcaatgc gctgatcgcc gaggccattc
    13021 gcgacatcgc gccgaacgac cgcatcattc tggataccgg cgacggggtt tcgatcaatt
    13081 tcctgggcga ccccgaggat gcgctgttcg tggcgatgag cctgcgcgag tcgttcgctc
    13141 ccaggcccaa cgaaccgccc aaacttccag cccgcatcgg cgtcaatctc ggcccggtca
    13201 ggctggtccg ggatctcaac gggcagccca acatcatcgg cgacggcatc aacgtggcgc
    13261 aacgcgtcat gggatttgcg ttgcccgggc agattctggt gtcgcgctct tacttcgata
    13321 tggtgtcgca catttccgag ggctacgcca agctgttccg caatgaaggt tcgcacaccg
    13381 acaagcacgt gcgagagcac gagatctaca gcgtcggcta cagcaacgcc gatgacacca
    13441 ccatacgttc tccggccgag cgcgcccagg tatcatcccg cgagggcata cgccagcagg
    13501 ccaccgcaac cattgccacc gggaccggcg gctggctggc cgtaatcgaa ccctggctga
    13561 acaacaggac cattgcctac ggcaccgcaa cattgtcggt gggcgctttc gcgctggcgg
    13621 tcctgcttaa ctttctcggc cagagcaacc tgccggcggc gccggtgaag cacgaagccc
    13681 agacagcgca gaagaaatcc cacgccgccg gcaaagccga ggaacccgct gtttccggat
    13741 ttcccgggcc gacggagaag aaacccgctg ccagggaagc cgccaagcca gccggtaccg
    13801 gcgtcatcgc gctggcgatc gcgccatggg gggaggtcta tgtggacggc gaccgcgtcg
    13861 gagtcagtcc gccggtaaac gaagtggaag tcgcaccggg caggcgcaag atcgaaatcc
    13921 gcaatggcag attcccggtt tacacccagg tagtcgacgt gaaagcggac cagaaagtgc
    13981 gcatcaagca caagttcaac tagaacaata cgaaaccatg cccacgatac gacttgcact
    14041 gatcctgatc ctggccgcga atcttgcagg cggctgcgcg actggttcgt tccgggattt
    14101 attccatgcc aggacagccg gcagcaatta cctcgaggaa ggcgtccgca actacgagga
    14161 gggaaactac cggacatccg cccggcggat ccagtacgcg ttggaagaag gcctgagccg
    14221 ccctgaccgc gtcaaggcgc acaaatatct cgcgttcatc gcctgcgtgt caggccaaca
    14281 gctcacctgt cgcgaggagt tcagcatcgc actggagctc gatccctcgt tcgaactcga
    14341 cgccgcggaa gccggacacc cgatctgggg gccggtatac aagagcgtga aggcgaaaca
    14401 gaagtgatgg atgtcgcccg atgagtccga atagctcgat cgccacactg ggtcgctacg
    14461 aaatcctggc cgaactcggc cagggtgcga tgggcgtggt ctacaaggcg cgcgacccga
    14521 tgctcgatcg cgtggtcgcg atcaagacgg tgaacctgac cctgcccaag gacgaactgg
    14581 cggagtacga agcgcgcttc taccaggagg ccaaggccgc cgggggactc tcccaccgca
    14641 acatcgtcac catctacgac atcggccgca gcgaacgcat cgcctatatg gctatggagc
    14701 ttctcgaagg cgatgaactg cgcaagctgt tgcaggcgcg cgcaccgatt ccgctttcac
    14761 gtgcactgga catcgccgcc caggtcgccg aggggctgca gtacgctcat gagcgccaga
    14821 tcatccatcg cgacatcaag ccggccaaca tcatggtgtt acaggacgga ctggtgaaaa
    14881 tcaccgattt cggcatcgcg cgcatgcgca ataacgaagt caagaccatg accggcatga
    14941 tcctgggttc ccccaagtac atgtcgccgg aacaggtgtc gggcaagcgc gcggataccc
    15001 ggtccgacat cttttccctc ggcgtggtga tttacgagat gctcaccggc acttcgcctt
    15061 tcgtcgccga caacatccac ggcgtgatgt atcagaccat gaacttcaat ccgccggcgc
    15121 cgaaaacgct caatcccgac cttccggagg tgctgaattt catcattgcc aaagccctgg
    15181 cgaagaatct cgacgaccgt taccagaagg ccaacgacct cgcccacgat ctgcgcgagg
    15241 ccatggcgac gctgatcggc gcgtccgcga cgccggaggt ggtctcaggt ccggtcgaag
    15301 gcgggccctt tgtgctgccc gaatctgcgc tcacccgcca gcaagacagg gccgcgcgta
    15361 tcctcggcac tgccggcgcg cagcaggagc aacagtccgg cgaagagcaa ccggacccct
    15421 ccgcctcgga aaccacgcag ccggcgctgg gcttgtcgaa ggcgttcgat tccttcgatg
    15481 ccaccatgcg gctggcggcg ctgaccggga tggagaaaga gctggacgag ttctccgaga
    15541 cacagaaaat cgcgcggctc aaggcgctgt cgcaggccaa gcgcgcggct gcaccggcga
    15601 cctccccgtt gtccgccagc atgacctggc agcgatccgg cagcctgatt cgatcggtgg
    15661 gcagcggcgg ccagtccgcc agcgccaacc tcaaggtggt gtggataggc agcgcgctgc
    15721 tggcgcttgc cgcggccgcg ctgttcttgc tgcattgagg tacttcgtgt gattgagtga
    15781 ttcagtgatt cagtgattca gtgattcagt gattcagtga ttcagtgatt cagtgaccag
    15841 acctttgaac tcactcaatc actcaatcac ctgatcactg aatcactgca tcacccgtca
    15901 ccgagatctt gccaccgctt cctcgacccg atccaccgtc accacttcga ttccttcgat
    15961 cgccgtcctc ggcgcgttcg ccttgggaat gattgcggtc ctgaacccaa gcttggcggc
    16021 ttcacgcaat cgctcctgcc cgcgctgcac cggccgcacc tccccggcga ggccgatctc
    16081 tccgaacgcg accagctttt ccggaagcgg cttgttccgc agcgacgaca cgattgccag
    16141 cagcaccggc aaatcggccg ccggctcgtc gattttcact ccgcccacgg cgttgacgaa
    16201 cacgtcctgg tcgaaagccg caatgccggc gtggcggtgc agtaccgcga gcagcatcgc
    16261 cagccggttt tgttccagtc cgacgctcag ccgccgcggg ttcggcgcgt gcgcttcgtc
    16321 caccagcgcc tgaacctcca ccagcagcgg ccgcgtgcct tcctgggtca ccatcacgca
    16381 ggaacccgcc accggggtgc cgtgttggga caggaatagc gccgagggat tggacacgcc
    16441 gcgcaggccc cggtcggtca tggcaaacac cccgagttcg ttgaccgcgc cgaagcggtt
    16501 cttggtcgcc cgcaccagcc gaaaggccga atgcgcgtcg ccctcgaagt acagcaccgc
    16561 atctaccatg tgctccagca cgcgcggtcc ggcgagcgcc ccttccttgg tgacgtgccc
    16621 gaccagcaac agcgtggcgc cggaagtctt ggcatagcgg gtgagttgcg cggcgcattc
    16681 gcgcacttgc gcgacgctgc ccggcgccga ctgcaacgct tcggaataaa tggtctgaat
    16741 cgagtcgatc accgccacat ccggcttctg cgccgccagc accgacagca ctttttccag
    16801 ctggatttcg gccagcagat tgagcgtctc ggcaccgccc gcaaggcgct tggcgcgcag
    16861 ggcgatctgc tggaccgatt cctcgccgct gacgtacacc gcgttgcacg agccgcccat
    16921 gagcgacaac gcctgcagca gcagcgtgga tttgccgatc ccgggatcgc cgcccagcag
    16981 caccaccgct ccgtgcacca ggccgcctcc gagcacgcgg tcgagttcgc cgatcccggt
    17041 tggccggcgc ggctcctcct gcgcctcgat gtcggcgagt ttttgcagtt tgctcgactg
    17101 cgccagtgcc tggaagcgcg acgaggcttt ttccgcgatg gtttccacca gggtgttcca
    17161 ggcgctgcaa tgcgggcatt gcccctgcca tttgagcgtc tgcccgccgc actcggtgca
    17221 ggcgtaaacg gtcttcgcct tggccatcgc gtcagtctcc gcggtgctcc tcgaccggga
    17281 cgcgccgcgc cagcgcacac agcaactcgt agctcaccgt gccggcagct gccgccactt
    17341 cctccaccgg cagccccgct ccccacagcg tcaccgggct gcctattccc gcccgcggaa
    17401 tatcgctcaa gtccacgcac agcatgtcca tcgaaacccg cccgaccatc ggcacccgca
    17461 ccccatcgac caccaccggc gtgccgttgt aggcatggcg cggatacccg tcggcataac
    17521 cgcaggcgac cacgccgatg cgcgtttccc ggtgagcggt gaacagactg ccgtagccca
    17581 ccgtgtctcc gcgcttcagg ttctggatgc cgatgatctc gctggtaagc gacatcgccg
    17641 gcctaaggcc gatctgcgcc gccctgcggt cctcgagcgg gcttgcgccg tagagcatga
    17701 tgcccgggcg cacccactcg ccgcgcgcct ccgggaaacg caacagcgcc gccgaattgg
    17761 cgagcgagcg cgcatagtcc gcacgcgcga catggcgctc gaattcccgc atctgccatt
    17821 cgattccgct gacgccctcg gcgtcggaaa agtgggtggt caggaccact tcaccgacgc
    17881 acggcagcgt tttcaattgt tcgagcacgc gaccggcgcc ggcggccggg aagccgagcc
    17941 ggttcatgcc agtattgaat ttgacgaaca cttcgagctt tccgtcaccg tgcaatttcc
    18001 caagcatcgc catctgctcg tcgttgtgaa tcaccacgga aaacctctgg tgcaggatca
    18061 ccggcagttc gtcggcccgg aaaaaacctt ccagcagcag gatgcgctgg cgatagccgg
    18121 cgtcgcgcag ccgcaccgcg tcatcgagtt ccagcagcgc aaatccatcg gcatcggcga
    18181 gcgcacgtgt aacacgcagt aatccgtgcc cataggcgtt ggccttgacc accgccatca
    18241 ctttggccac gccggccagg cgccgggcga tggcgaagtt gctcctgagc gcggcgagat
    18301 cgaacgaggc ctggatcggg cgggccatgg aatttgttgt tgtagaggca ggaaaagggg
    18361 ccgaactgcc gctattctat gcgcttagcc gtcggcaacg aacaaatcga gtcggcgtcc
    18421 ggcttcgagg gattgcccgc acttctatgg tataaagccg ccggcctgcc attaccggtt
    18481 acgctccgac agaatccagg aaacagcgcc ctctgcctcc ccgacctcat gaatcgcggg
    18541 ttctacacca ttctggccgc ccagtttttc tccgcgctcg cggataacgc gctgttgttc
    18601 gccgccatcg cgctgcttgc gtcgctgcat gcgccggcgt ggcagacgcc ggtgctgcag
    18661 cagtttttcg tgtttgccta tatcgtgctg gctcccttcg tcggtccctt cgccgattcg
    18721 ctccccaaag gccgtgtcat gttcgtggcc aatgcggtca agcttgccgg ctgcctggcc
    18781 atgctgctgg gcatgcatcc gctgttcgcc tatggcatcg tcggggtcgg tgcggcgatg
    18841 tactcgccgg caaagtacgg catcctcacc gaatatctgc cgcatcagag actggtgttc
    18901 gccaacggct ggatggaggg cctgaccgtt gccgccatca tcctcggcgc aatcctcggc
    18961 ggcattatgc tcgggccgga gtttgccgac tatgtgctca agcacgggga gcttccagga
    19021 gtggatctgt ggatagacac cgcgcccgaa gtcgccatcg tctgcattct gacgctttat
    19081 ttcgtcgcgg cgatcatcaa cctgtacatt ccaaaggtcg cgatcgatca ccggctgccc
    19141 aagcgcaatc cgttgttcat cctgcaagac ttctggcaca gcctcagctt gttgtggcgc
    19201 gatccgctcg gacaggtttc gctggcggtg actacgctgt tctggggcgc gggggcgaca
    19261 ttgcggctga tcgtgctcac ctgggctgcg ctggccctga agttcgacct caagagagcc
    19321 acccagctta ccgcggtcgt cgccatcgga atcgcgctcg gctccatact ggccgccaaa
    19381 tacgtgagcc tgcaacgcgc ggtgcgggtg ctgccggtgg gaatcgccat gggcctggtg
    19441 gtggtgtcga tgatgttcgt caccgactgg cgggtcgcga tcgggctgct ggtgctggtc
    19501 ggcgccatgg gcggttactt cgtcgtgccg atgaacgcgc tgttacagca ccggggccac
    19561 ctgctgatgg gcgcagggca ctcgatcgcg gtgcagaatt tcaacgagaa cctttcgatc
    19621 ctgctgctgc tcggcatcta tgcgctgatg atcaaatccg agctatcgat gaactcgatg
    19681 atcgtggcgt ttggtttgtt tatcacgaca acgatggcct ggataaccat caagcactgg
    19741 cacgatcagg atccgcagta gttcagagca attggctcag cgcgatcggc gcttgcgcag
    19801 aagtgcccac agccacaatg ccccgccgca cacggcaatc aggatcagaa tcaccgcttc
    19861 gtagcgccgc aggtcgctaa ggaaggcctc catggcggta ccgaacagat agcctgcacc
    19921 gccgatcgcg gccgcccagg cgcctgcacc cagcaggttg aaacagacaa agcgccacgg
    19981 gcgtacgccg ctcatcccga tcagcacggg gccggcaatc cgcaatccgt agataaaacg
    20041 cacggaaaaa atcgcggcgg cgtcgtaacg cgtcaacaac gactgcactc gcgtactcga
    20101 ttgcaccagt gaagggaaac gcgacagcat cgcccggccg tgccgccggc ccatgaagaa
    20161 gaagaactgg tcgccgagga agccgccgac agctgctagc gccagcaccc acggcaactc
    20221 cagcagcccg cgatgagcgg cgaagccgcc catgaccaga acggtttctc cttccagcag
    20281 cgctcccaca aacaccgcca ggtaaccgta gtgctcgaca aggtgctgaa tcacccgcga
    20341 cgacccgtct cagagcacaa ccggtcggtc cggaagctcg ttcggccccg gtacgttgcg
    20401 tggaaaatgc gtctgcaggt gacgcgcaac ccgctcgatt ccgtcaagca cgccgtactc
    20461 gaaacggccg acccggaacg cagcctccat ctcacggcag atatcctccc agccctggct
    20521 gccgactttc ggatgaatgc cgcggtcggc gacgatctcg acgtcacggt cggcaagcaa
    20581 caggtaaatg agcacgccgt cgttgtgttc ggtgtcccag actcgcaacg ccgcaaacac
    20641 ttcgatggcg cgctcgcgcg ctgattgacc gcgaagcaag gcatggagat cgagcgcgtt
    20701 ttcgacggca aaccggattt gtccggagcg gcgcgactcc gattcgccaa tggcatgctc
    20761 gattcgcagc agtgctcccc tcggcaatag ctggcgcgcc cgccagtgcg gcatcgtcag
    20821 gtgacgcaac atgcgcgcga gtttcatttc accagcgccc cgaagcacca ccgccgccaa
    20881 acccgccgcc gccgccgctg aatccgcccc cgccgaaacc cccgcccccg aagccacctc
    20941 ccaccccgcc gccccatccg ccgcgatgcc cgccgccagc cagcgtaaaa ataaagccga
    21001 cgatgcccac cagtaacgcg accaccaccg gcgcgacgat cagccaggcg agcaagccgg
    21061 taccggcgcc gacgatcgtc gccgcgggca gccgcccgaa cagcgttcgc aacaagccgc
    21121 caaccgcgat tacgattgcc atgaacagga cgaaataggc ctcgatcgag cgcggctcgc
    21181 tgccacgacc agcttcctgc ttcggcgcag gcagcggctc gccgtcgatg actcgcacca
    21241 ggcgatcaag ccccgcgcgc aagcccccat aaaaatcgcc ctcgcgaaat ttggggatga
    21301 attcttcctc gatcaggcgt ttggcgacgg catcaggcac tgcgccttcc aatccataac
    21361 cgacttcgat gcgcatcgcc tggtcgctct tcgcgatgac cacgacgatg ccatcgtcca
    21421 cgcccttgcg gccgatcttc catgcctccg ccacacgcat ggcaaatgat tctatcggct
    21481 cgggaaaggt tgttccggtg atcagtaccg cgatctggct gcccttgcgc ttttcgaacg
    21541 cttccaggct cgcttccagt tcacgcttct gttcggcgct cagcgtgcta ttcaggtcca
    21601 ccacgcgccc ggtcagaggc ggaattgacg cgtcttccgc cacggcacag aaggcaagca
    21661 gcagcgcggc cgccgctacg gcgcgcatca gcagcatcat ttcgtcactt gttctgcgcc
    21721 ggttcgggct tggagaaatc caccgtcgga ggcttggaga tcacttgttc gttttcgacc
    21781 gtgaaagtcg gcttgacctt gtagccgaac agcatcgccg tcaagttgct ggggaaggaa
    21841 cgcacggtga cgttgtagtc ctgcaccgcc cggatgtaac gctggcgtgc cacggcgatg
    21901 cggttctcgg taccttcgag ttgcgcttgc aggtcgcgga aattggcatc ggatttcagt
    21961 tgcggatagt tctccgccac cagcaacagc cgcgacagtg ccgaggtcat cgccccctgc
    22021 gcggcgatga acttggagaa agcctccgga tcgttgacca gttccggagt agcctgcatg
    22081 ctcccaacgc gggcccgggc attggtcacg cccagcagca catccttctc ctgcgcggcg
    22141 aaacccttca cggtattcac cagattcggc accagatcgg cgcggcgctg gtactggttg
    22201 agcacttccg cccacgccgc cttgatctgc tcgtcgcggg tttgcagcgt gttgtagccg
    22261 cagccggcga gaccgaacga aataaccagc aggaacggca gggcaagaaa acgtttcatc
    22321 aaattctccc ggttggaggc gacaacgatc aattgggggt gctgccgccc tgtttcaacg
    22381 ctttcatggt gtgcaccgcg aagcataggt cttcccaggc cttggccttg tcggtgaggt
    22441 tgcgcagcag gtaggctgga tggtaggtca cgatcaaggg cgtgccgcga taagcgtgcg
    22501 cacggccgcg caggctggca atggtcgctt cggtgcccag caggttctgc gccgcgatct
    22561 tgccaagcgc gatgatcaga cgcggcttga tgagcgcgat ctggcgtgcg aggtagggtt
    22621 cgcaggcatt ggcctcggcg ggctcggggg tgcgattgcc cggaggccgg cacttgacga
    22681 cgttggcgat gtagacgttg tcgccgcgct tcaattcgat cgcggcgagc atgttgtcca
    22741 gcagcttgcc agcctgaccg acgaagggct ctccgcgcgc atcttcttcg gcgccgggac
    22801 cttcgccgac gaacagccat tcggcgcgct cgtcgcccac tccgaacacg gcctggcgcc
    22861 gctgcttgtg cagaacgcat gaggtacagg cggcaaccgc ggacttgagc tgttgccagt
    22921 tcatcgcccc gacttgcgcg ccgcggtcat ctgcggggag cagttgcgcc gttggcagcg
    22981 gtggcagatc gcgggtcacg gcgttttgaa ccgcgtccgt ttccccctca gccgtcgccg
    23041 cgcgcctaag cacccacaaa ggcgtgagtc ccagttcggt gaggaccagc gcccggcggc
    23101 tcacagcatc agtcccatca ggatcgcatc ctcgcgcccg ccagctaccg ggtaatagcc
    23161 gcggcgaacg ctgatgtcgc gaaagccgaa atccgcgtac agtcgccgcg ccgatgcatt
    23221 ggaaggtcgt acttccagga acatctgttg tgcgtcgctg tcgcggccaa tgcgcacgaa
    23281 atgctccagc agcgagcggc ccagcccgcg cccctgccag ttcggcgcga cgctcaggtt
    23341 gagtagatgc gcctccctga cgccgatcat gagcacaccg tagccgacca ccaccgcgct
    23401 gcagtcgatc acccagcagc tgtagccgac tcgtatcgaa tcgtcgaaat tgccccgcgt
    23461 ccacgggtgc gtatagatga ccggctcgat ctccatgatc cgatcgagat cggaggcgcg
    23521 catagggcgc agtttcggca ggctgtcaag ctgggcactc atggttgttc gtgcatttta
    23581 cgggcgacct tgtcgcgcac atagagcggt accgcctgtt ctgccggaac accgtggccg
    23641 gcggcaagga ccggcaacgc cagctgcgcg acctcccggg cgtgcggaaa ggcctgcgac
    23701 tgcacgcgcg caagctggcc atgatagcgc gcagccaagg catcgccgta caccgcgaaa
    23761 gcgctgccgc aacccaccca gtccctgccc tccagtgccg gcgctgcgtc ggccttgcat
    23821 acgttgggca tcaccgtttc gctccatccc gcgccgttgc gctggtaggc ggcatgataa
    23881 atctctccca tgcgcgcatc gaggcaggcc accaccctcg gtgcgccggc ggcctctgcc
    23941 agcgctagca gcgttccgac cggcacaaca ggaagccccg caccgaaagc caggccctgc
    24001 gcaactccgc aggcgatgcg caggccggtg aacgatcccg gtccggcgcc gaaggcgatg
    24061 ccatgcagat gcgccaaccc aagattcgat gtgcgcaaca gcgcgtcaat catcgccaga
    24121 atcagttccg agtgcgtctg tccagcgagt tcttcgcgga aagtgagctt tccgtcatgc
    24181 agcagcgcga ccgagcagta ttccgtcgaa gtgtcgaggg cgaggatgtt caaggagggt
    24241 ccatcagcgc ttacgaagcg cgaaacaagt gtcctgttcc gctgattcgt gggacaactt
    24301 gcgaaccttg gagcgcagac cccatatcca tttggcaaca atccatcatt gatcggaaaa
    24361 cccgcgaaat cggtgtcagg caaggcgcga gtcctcgaca gtggcggggg ccggtggaac
    24421 gtggagcagg gccccgcttg cggggatgcg accgtgcaac cggccaatag cgtgttgagc
    24481 accggcctga ctcgtgtctc cgcggtcaga tccccggagg ggcccctctt ccgcgctccg
    24541 acacgcacgg cgaggactgg taacactgca tggcgccgat tctcgccggg ttttcagccg
    24601 cgaattagcg gaacaggacg ctagacacgc agattgtaga cgaatgagga cggacaacag
    24661 ctggcgttgc caaacgggca tacttgcgta aatattcggg gctcaagccc gttgccgtga
    24721 ctgagaattg cagattgacc gaggagcgtg tctatggctg gcgagaacaa gaccaaagtg
    24781 accatcctga ccggaaccta ccggatcaag gggcacattg aattgaccct g
//
LOCUS       DASQOP010000176        25858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_476024, whole genome shotgun sequence.
ACCESSION   DASQOP010000176 DASQOP010000000
VERSION     DASQOP010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 25858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25858
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476024"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /gene="pilO"
                     /locus_tag="VE030_07145"
     CDS             <1..280
                     /gene="pilO"
                     /locus_tag="VE030_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HZF23223.1"
                     /translation="PAQKENMSEFYAELPITIKLTGKYHDIGAFASDVSQLSRIVTLN
                     DIALTPNQAGDLVLDAVAKTFRYLDESETAAQRKAARDAAAAQRGGAK"
     gene            277..795
                     /locus_tag="VE030_07150"
     CDS             277..795
                     /locus_tag="VE030_07150"
                     /inference="COORDINATES: protein motif:HMM:NF016254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HZF23224.1"
                     /translation="MRRSLIGLAVAALLVTGCGRDEFSDLKQFVKESGEGLRGRVEPI
                     PEVKQFEPFAYSAFDLPDPFKPRTTVVDSRTPGGGPQPDLNRRKEALEAFPLESLQMV
                     GTLEQKRVQYALIKTPDKSLYRVKAGNYVGQNFGVITTVSDSAVTLKEVIQDPSSGSW
                     TERTSSLQLLEQ"
     gene            813..2942
                     /locus_tag="VE030_07155"
     CDS             813..2942
                     /locus_tag="VE030_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HZF23225.1"
                     /translation="MKRVGRLVHMVAAPIGLLLVLSSLAAHGQDSKTGNGGTGNVIQS
                     IDVSTQAGTVTVRLGLKEAMPNPPAAFTVNNPPRVALDFPNTANGLGKSTQDVNQGDL
                     RTIRFGQSGGRTRVVFNLAKMLKYDTTVEGNTVVVTLQGGGATSAAAATTQFAESKSS
                     GDQHSVQDVDFRRGKNGEAQIVVDLSDTATGIDLRQQGRTVLVDFLQTMLPPALERRF
                     DVTDFGTQVDFFEVTRQGNNTRITVTPKGRWEQSAYQTDKRFIIEIKPVIEEAGRPKE
                     GKGYTGEKLSLNFQNIEVRAVLQVIADFTSLNIITSDTVSGNLTLRLKDVPWDQALDI
                     ILQAKGLDMRKTGNVVWIAPRDELATKEKLALEARQQIADLEPLRTESFQLNYQRAED
                     IQKIITASDQKLLSKRGSAVVDPRTNTIFVQDIVDKLDDVRRLLKQIDVAVRQVLIEA
                     RIVEASDTFSRNLGARLGFVDATGRGERIGGTHRITAGGGLASTGALTGQISGTPDFF
                     TDGLSTNMPAPGLGGVLPGQFSLILMNDAMSKFLNLEITALQADGKGKIISSPRVITA
                     DNVEATIEQGTEIPYQQATSSGATSVSFRKATLSLKVKPQITPNDNIIMKLHVNKDSV
                     GANTSAGPSIDTKQISTEVLVDSGGTVGIGGIFEQEESSTRTKVPMLGDIPVIGILFR
                     QDLRRNDKRELLIFVTPRVVKDTLTVR"
     gene            3062..3619
                     /gene="aroK"
                     /locus_tag="VE030_07160"
     CDS             3062..3619
                     /gene="aroK"
                     /locus_tag="VE030_07160"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HZF23226.1"
                     /translation="MQARVDLPGSIFLVGMMGAGKTSVGRVLAKRLQKTFYDSDHVIE
                     DRTGVKIPVIFEIEGEAGFRVRESAVVDELTALCDIVLATGGGAVLSEKNRERMRTRG
                     MVVYLRASVRDLLNRTRHDKNRPLLQATDPRVRLTELYEKRDPLYREVAHLTVDTGNQ
                     SLTSLVNRLCQLLLETRSEQRTQAP"
     gene            3632..4720
                     /gene="aroB"
                     /locus_tag="VE030_07165"
     CDS             3632..4720
                     /gene="aroB"
                     /locus_tag="VE030_07165"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HZF23227.1"
                     /translation="MRTITVGLGERSYPIYIDRGLLARTELLLPHLGQRKVVIVTNST
                     IAPLYVEPLTSALGNAAVEVVPIVVPDGEDHKNWQTLNTIFDLLLTHRCERRTTIIAL
                     GGGVVGDLAGFAAATYQRGLPFIQMPTTLLAQVDSSVGGKTAINHPRGKNMIGAFHQP
                     RLVVADIATLDTLPARELSAGLAEVIKYGLIMDAPFLSWLEVNMEKLRVHDVDALAYA
                     VARSCENKARVVAADEREAGARALLNLGHTFGHAIEAGLGFGTWLHGEAVAAGIILAA
                     RLSERMGLLNSAEVERVARLMSRAGLPTEAPDLGVARYLELMGHDKKVQDGKLRLVLL
                     RALGEAFVTSDFHPEDLRQVLTGAAAHA"
     gene            4713..5834
                     /locus_tag="VE030_07170"
     CDS             4713..5834
                     /locus_tag="VE030_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HZF23228.1"
                     /translation="MRELAAYGSDPAASRGRRIAEPAPTGRTEFQRDRDRIIHSAAFR
                     RLEYKTQVFVNHEGDLFRTRLTHSIEVAQIGRSVARNLGLNEDLVEAISLAHDLGHTP
                     FGHAGQDALNECMKEYGGFEHNLQSLRVVDLLEQRYGAFDGLNLCFETREGILKHCSI
                     KNAEKLGGVGERFLKNQRPSMEAQLANLADEIAYNNHDVDDGLRSALISVDQLSSVRI
                     FARHLDAVRGQYPELVDRRLIHETVRRMINTLVSDLTERSRALIAEHRPDSVDQVRLA
                     PPLIAFSPAIREEQHELKKFLRKHLYQHYRVARMSNKAKRIVRDLFQAFMADPRLLPP
                     EFQSRAEADLPRAIADYVAGMTDRYAIREHRRLFAVEES"
     gene            6011..10663
                     /gene="gltB"
                     /locus_tag="VE030_07175"
     CDS             6011..10663
                     /gene="gltB"
                     /locus_tag="VE030_07175"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010092911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HZF23229.1"
                     /translation="MTNPALPARQGLYDPANEHDACGVGFIAHIKGAKSHTIIEQGLQ
                     ILKNLTHRGAVGADPKAGDGAGILIQIPDPFFREELAKQGIKLPPPGEYGVGMVFLPQ
                     EPASRLACEYEIERAIKDEGQVLLGWRDVPRDGSSLGESVKRIEPVIRQVFIGRGAGV
                     TVTDALERKLYIIRKVSGHAIQALKLEHGKEFYVPSMSARTVLYKGMLLAGQVGSYYK
                     DLQDARVVSALALVHQRFSTNTFPTWDLAHPFRMIAHNGEINTLRGNVNWIRARQGAI
                     SSPILGRDLDKLWPLIYDGQSDSASFDNALELLTMGGYSIAHAMMLLIPEAWEKHAHM
                     DPSRRAFYEYHAPMMEPWDGPAAVAFTDGRQIGATLDRNGLRPARYLVTSDDMVVMAS
                     ETGVLPVPEEKIIKKWRLQPGKMFLIDLEAGRIIDDKELKDTLANAKPYKAWIDRIRI
                     KLDDLPVSTKIVEQSKVPLLDRQQAFGYTQEDLKFILSPMAQQGEEAVGSMGNDLPLA
                     VLSHKNKTLYHYFKQLFAQVTNPPIDPIREELVTSLVSFIGPKPNLLGIDESNPPMRL
                     EVSQPVLDFNQMETIRHIDYYTGGKFKSFEIDITYPVAWGNHAIEARLASLCAQAEDA
                     VRSGYTILVVSDRKLDATHVAIPALLALSAIHQHLVGKGLRTSAGLVMETGSAREVHH
                     FALLAGYGAEAVHPCLAMETILHADESIPDAYRGEKGVANYVKALGKGLMKVMSKMGI
                     STYMSYTGAQIFEAVGLAKVLVDKYFTGTTSSVEGIGVFEVAEEAIRQHRAAFGEDPV
                     LGNALDVGGEYAWRVRGEEHMWTPDAIARLQQSTRSNSYTTYKEYARIINDQSKRHMT
                     LRGLFEFKVDGRVPVAIEEVESAAEIVKRFATGAMSLGSISTEAHTTLAIAMNRIGGK
                     SNTGEGGEDYRRYAPVKAGESLMSRFGRERVEVDIPLAAGDSLKSKIKQVASGRFGVT
                     AEYLVSAEMLQIKIAQGAKPGEGGQLPGRKVSAYIAELRYATPGVELISPPPHHDIYS
                     IEDIAQLIHDLKNANPDAAISVKLVSEVGVGTVAAGVSKAKADHVTIAGHDGGTGASP
                     WSSIMHAGTPWELGLAETQQTLVLNRLRGRIAVQVDGQLKTGRDVVIGAILGADGFGF
                     STAPLVVEGCIMMRKCHLNTCPVGIATQDPALRRKFEGKPEHVINYFFFVAEEARELM
                     AKLGIRKFNDLIGRTDLLDTRKGIEHWKARGLDFSKIFCIPQMPADVARYNIEKQYHG
                     LEKALDHRLIELAKPALQRGEKVTIEMPIRNINRTVGTMLSGEIAKRYGHDGLPDDTI
                     HIRFAGSAGQSFGAFLAKGVTLDLIGDTNDYCGKGLSGGRISVQPSPKFRGEPTENII
                     TGNVVLYGAIAGEAYFRGVAGERFAVRNSGARAVVEGVGDHGCEYMTGGTVVVLGMTG
                     RNFAAGMSGGIAYVLDEDSSFKTRCNPSMVQLEPVLSEAEQEAKVPQDVWHMNRADET
                     ILKGAIEKHAGYTASARAREILSNWAAYRTKFVKVFPSEYRRALGELAAKRRELAA"
     gene            10753..12216
                     /locus_tag="VE030_07180"
     CDS             10753..12216
                     /locus_tag="VE030_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HZF23230.1"
                     /translation="MGKITGFMEYQRLDEPHEPKEARKKHYREFYARLSDEAAATQGA
                     RCMDCGVPFCMSGCPVNNIIPDWNDLVYRHNWKHAIDVLHSTNNFPEFTGRICPAPCE
                     EACVLRINADAVSIKSIEHAIIDKAWEEGWVKPQPAAHKTGKKIAVVGSGGAGLACAQ
                     QLARAGHEVTVFEKSDRIGGLLRYGIPDFKLDKSVIDRRLDQMRAEGVEFRPRVFVGV
                     RSPGKGVVNDARETIAPEKLMAEFDAVVLAGGAEQPRDLPIPGRSLDGVHFAMEFLPL
                     QNKRVAGDSSVPELWGTNKHVVIIGGGDTGSDCVGTSNRHGARSVTQFELLPMPPDVE
                     RNPVWPFWPTRLRTSSSHEEGVNRDWSVATKEFIGSNNRVTALRAVRLEWKEGKPHEV
                     PGSQIDMPADLVLLAMGFVSPLQSMLEEFGVEKDARSNVKADTEGPKAYATSVPKVFA
                     AGDMRRGQSLVVWAIREGRQCARAVDEFLMGDSVLPR"
     gene            12404..13465
                     /gene="hemE"
                     /locus_tag="VE030_07185"
     CDS             12404..13465
                     /gene="hemE"
                     /locus_tag="VE030_07185"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HZF23231.1"
                     /translation="MSKLKNDTFIRALLRESTPYTPVWIMRQAGRYLPEYNQARTRAG
                     SFLDLCKNPDLATEVTLQPLARFPLDAAILFSDILTIPDAMGLGLSFAEGEGPRFERP
                     LRDERQIRNLRVPDPDMHLRYVLDAVSQIRRALAGEVPLIGFSGSPFTLACYMIEGRG
                     ESDFHETKRMLYQRPELLHHVLDVNARAVAAYLNAQIESGAQAVMLFDTWGGALSGEA
                     FHEFSLCYIEHVFAQLRREHDGEQVPRIVFTKGGGQWLESLAHCGADAVGLDWTVDIG
                     KARARVGDRVALQGNLDPAVLLSAPEVIRREVGKILAGFGHGNGHVFNLGHGVSQFTP
                     PEHVAALVETVHSGSPQFH"
     gene            13804..15978
                     /locus_tag="VE030_07190"
     CDS             13804..15978
                     /locus_tag="VE030_07190"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012327608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HZF23232.1"
                     /translation="MTIARIALDVPLETLFDYRTGDLAPVPGQLVVVPFGRRRDVGVV
                     VELADRSEIADARLRRIERFLPLDPLPEDLLALTRFCSDYYHHPFGRALLSTLPSLIK
                     RAGYSGPKRQWQYALTSAGRALRADQFPPGAAVKRRLLAALSTSAALGQAQVRALSAR
                     APALLQQWMEAGWVEKRPATIPDAPVSRSEQGPQLTGEQRRAVEMISATFGTFSPWLL
                     DGVTGSGKTEVYFRLIEAAIVHGSQSLLMVPEINLTPQLEARFLQRFPGVRLVSLHSG
                     LSDGERCERWLAAFAGDTQVVLGTRLSVFTPLPRLGLVIVDEEHDASFKQQEGLRYSA
                     RDVALFMARQRRVPIVLGSATPALESYHNARSGRFGHLHLSTRPAASPPQIRLIETRA
                     ARQTHGMSEPLLQAIRERLLRGEQSLVFLNRRGFAPALVCGACGWVAQCGRCAARLVW
                     HLRKERLRCHYCGHEEHLSQVCPDCGNQDLKGLGQGTQRLEEALIGIFPGARILRIDR
                     DSTRRKRSWQAMREDIHGDRVDILVGTQMLAKGHDFPKLTLVAVVNPDSALFSADFRA
                     AEKLFQQLMQVTGRTGRADLSGEALVQTQFPAHPLYQALLLQDYRAFADELLAERKHS
                     GFPPFVYQALLRAEALSERALMDFLTEAVHRAAGHDEDVTLYDPVPAAIARVAGHHRA
                     HLLVQSSSRGALQRFLSNWVPALAQKKAGRVRWSLDVDPLEL"
     gene            16034..17779
                     /gene="argS"
                     /locus_tag="VE030_07195"
     CDS             16034..17779
                     /gene="argS"
                     /locus_tag="VE030_07195"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HZF23233.1"
                     /translation="MSNAPALDLRSHFTGLLRSALVEIAPDAANLPLILERPKSAQHG
                     DYACSVAMQLAKPMKRNPRDIAQALVKALPQSPYLEKAEIAGAGFVNLYLKPAAKQQI
                     LRLVLQQRHAYGRVDVGGGRKVQVEFVSANPTGPLHVGHGRGAAYGASLASLLEAAGF
                     AVTREYYVNDAGRQMDILALSTWLRYVEIAGARLEFPANAYQGDYVRDMARQIFEAHG
                     KRYLHPVESMMRDAPPAQSDPEARLDYLIASAKRVLGPDYDYVHDFVLAEQLGDCRKD
                     LQEFGVTFDSWFSEKSLFDSGLVVRAVEQLQASGHVYRKDGARWFRSTVFGDEKDRVV
                     QRDNGQFTYFASDIAYHLNKFQRGFDLLVNVWGADHHGYIARVKGALQALRLDPAQLT
                     IALVQFAVLYRDGKKVSMSTRAGEFVTLRELRSEVGNDAARFFYVLRKSDQHLDFDLD
                     LAKSQSNDNPVYYIQYAHARVCSVLEQWGGDASTLVNADPGPLVGEQELEVLQKLLDY
                     PETIESAARDLAPHQLAFYLKELAAAFHSYYNSTRVLVDDESIRLARLALANAVRQVL
                     ANGLAILGVSAPEKM"
     gene            17816..18406
                     /locus_tag="VE030_07200"
     CDS             17816..18406
                     /locus_tag="VE030_07200"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HZF23234.1"
                     /translation="MSRDYKNSGSAAPNGRGNPMGVGILIGLLLGLCIALGVALYINK
                     GANPFVQKAKPVEVTGRETPATDAPKLPPPADKPAKTAEAKPRFDFYKILPGTEEAVT
                     DKEFKRTPPASSKEVYFLQVAAFQNPADADNLKARLALAGIEAQIQTATLPDGKVWHR
                     VRVGPFPNKDELGKSRAALKENNLEANLIKVREPTQ"
     gene            18432..19085
                     /locus_tag="VE030_07205"
     CDS             18432..19085
                     /locus_tag="VE030_07205"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HZF23235.1"
                     /translation="MNRFHTALMVASAFLCLVFAPAGLARPTPDVDYKAITPPQPVAG
                     KGVEVLEFFNYACSHCYDFEPLLKLWLKTKPKDVEFRYVPAVFNERMLPLAKTYYALE
                     ELELLDRMHERVYEAIHEKHVNLADRGVMMKWIAEQGVDARKFEATYDSFSVDSKAQR
                     AGQLTRSYRITATPYVAVAGKFLTAPSLTLRADGNIDHNRFFQVLNDLVIMGRGGAK"
     gene            19189..19740
                     /locus_tag="VE030_07210"
     CDS             19189..19740
                     /locus_tag="VE030_07210"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HZF23236.1"
                     /translation="MDYVLIPLQPGASGGRIEVIEFFYYGCDSCYRFEPMLSVWLNEP
                     APDVSFRRVPALRRMEWVPLTRLYFALEEAGALDRLHGEVYKAIHEKGINLGDAGELL
                     KWAEMNGLERASFGKILNSESMSAKVQKARDTTVAYGIRATPSIVVDGKYLTSGGMLG
                     SLDSLLPVVDGLIDKARRERAVK"
     gene            19737..20513
                     /locus_tag="VE030_07215"
     CDS             19737..20513
                     /locus_tag="VE030_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010090518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZF23237.1"
                     /translation="MTEAPLRVVITGASSGIGNALAHEYAKHGATLGLIARRGDLLTR
                     LAGSLPVRSYTYTADVRDARALAVAANDFIGRVGCPDVVIANAGVSAGTLTGNPQDNQ
                     VFEEILAINVTGMMLTFQPFIEPMKACEHGTLAGIASIAGFRGLPGAAAYSASKAAAI
                     SYLESLRVELKRSGISVVTICPGYIATPMTGSNPYRMPFLMDADVAARKIAGAIARRR
                     RLYVLPWQMAVAGWFMRRLPRPLYDSLFSRAPHKPRRSVT"
     gene            complement(20527..21180)
                     /locus_tag="VE030_07220"
     CDS             complement(20527..21180)
                     /locus_tag="VE030_07220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HZF23238.1"
                     /translation="MPNPAVRAVLWDFGGVMTESPFVAFRRFEQARGLPAHFLREINA
                     RNSDGNAWARFERSELTPGEFDRTYAAETRAAGHEVRGLDVIDLLYGEVRTEMVRALR
                     ACKQHYTNACVTNNVNAGPGHGFDRDPQRGAQCQTVLDLFDTVIESSKLGARKPEPRF
                     FELVCTQLRIAPEQAVYLDDLGSNLKPAKAMGMRTIKVEDPATALAELETALGIVLR"
     gene            complement(21188..21664)
                     /locus_tag="VE030_07225"
     CDS             complement(21188..21664)
                     /locus_tag="VE030_07225"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZF23239.1"
                     /translation="MKNPLKSLFGVLLAALLVAFVPLAAANLGDDDAAASDDPNFQEG
                     KKAVESQDWNKAVELLSKAVQSDPKNADAHNFLGYAYRKAGKFDASFNHYNEALKLNP
                     KHKHAHEYIGEAYLLADNLPKAEQHLAELQKLCTPIPCEEYKELKRAVDTYKKNKK"
     gene            complement(21754..23583)
                     /gene="glmS"
                     /locus_tag="VE030_07230"
     CDS             complement(21754..23583)
                     /gene="glmS"
                     /locus_tag="VE030_07230"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HZF23240.1"
                     /translation="MCGIVGAVARRNVVPILLEGLRKLEYRGYDSAGLAVVNGGMHRL
                     RSVGRVAALAEMAEQAKVHGSLGIAHTRWATHGAPTERNAHPHVSEDGLAVVHNGIIE
                     NHEQIRERLSAAGYKFVSDTDTEVVAHLIHLNLRRGGGLFAAVRKSAVELQGAYAIAV
                     LSEKHPGTLVAARKGAPLLLGLGKGENFVASDASALLQVTKRIVYLEEGDVAELKVDG
                     HHVFDAHGRETDRAIHESKLSADAVELGPYRHYMQKEIFEQPGAVANTLEMVTGAQSI
                     APQLFGVEAETMMKRVNAVTLLACGTSHHAGLVARYWIEAIAGIPCSVEIASEYRYRE
                     SVPNPDALIVTVSQSGETADTIAALQYAKSLGQNLSLAICNVAESTLIRLASLKFLTR
                     AGPEIGVASTKAFTTQLAALFLLTLVLAKLRGRLSAEQEKQHLHALRHLPSALQQVLT
                     LEPEVTKWAKRFSGKQHALFLGRGMHYPIAMEGALKLKEISYIHAEAYPAGELKHGPL
                     ALVDKDMPVIAIAPNDALLEKLKSNLQEVRARGGELYVFADADSRIQAGDGIHVIRMT
                     GHAGYLSPILHTVPLQLLAYHAALAKDTDVDKPRNLAKSVTVE"
     gene            complement(23676..25055)
                     /gene="glmU"
                     /locus_tag="VE030_07235"
     CDS             complement(23676..25055)
                     /gene="glmU"
                     /locus_tag="VE030_07235"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HZF23241.1"
                     /translation="MKSAPLNVVVLAAGKGTRMYSRLPKVLHALGGKPLLAHVLDRAR
                     ALHADRTIVVYGHGGDAVPAAFADPVLKFVLQEPQLGTGHAVQQALPHLLPGGSTLIL
                     YGDVPLTRNETLARLASNPAELSLLTALLDDPTGYGRIVRDADGAIERIVEEKDASAK
                     EKKIREVNTGMLCAASARLGEWLARLTNRNAQREYYLTDIVSLAIASRTPVTSVYPAE
                     TWEILGVNGKEQLAYLERVYQLECARELMRKGVTLADPWRFDLRGELVCGHDVSIDVN
                     CIFEGKVSLADDAKVGAGCVLKDAEIGAGTVIAPYSHLDRVVVGRHCRIGPYARLRPG
                     TQLADEVHVGNFVEIKATKLGHKSKANHLTYLGDSTVGRNVNIGAGTITCNYDGANKH
                     RTVIEDDVFVGSDTQLVAPVTVGRGSTIGAGSTITRDTPPGELTLSRSKQVSVPGWKR
                     PKKTVKSDE"
     gene            complement(25115..>25858)
                     /locus_tag="VE030_07240"
     CDS             complement(25115..>25858)
                     /locus_tag="VE030_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HZF23242.1"
                     /translation="LEVAEIRVWGRGAARRDCGRTLMLRPCALCGGLFLAAAAFAAGD
                     DGVPTRQLEFVPPPAGSYRLEHIMTAPDGTVLDTDDQPKPLTRFTTGKVTVLSFMYTS
                     CSDAWGCPFAYQVMQELKKSLDHETALLPHLRFVSLSFDPEHDTPEAMRLYGGSHMAD
                     TGGLPWYFLTTLSPVGLRPMLEGFGQDLSVVLDENGKSTGSLSHVLKVFLIDRRGSIR
                     EIYSTSFLLPQVVLNDIRTLLIEQGISVN"
BASE COUNT         4919 a         7963 c         8171 g         4805 t
ORIGIN      
        1 tccggctcag aaggaaaaca tgtcggagtt ctacgcggaa ctgccgatca ccatcaaact
       61 caccgggaaa taccacgata tcggcgcgtt cgcgagcgac gtttcccaat tgtcgcggat
      121 cgtgaccctg aacgacatcg cgttgacccc caaccaggcc ggcgatctgg tgctggacgc
      181 cgtagccaag accttccgct atctggacga gagcgagacc gcggcgcaac gcaaggccgc
      241 ccgtgatgcc gctgccgcgc agagaggcgg cgcaaagtga ggcgttctct gattggcttg
      301 gcggttgccg cgttgctggt caccggttgc ggcagggatg agttttccga tctcaagcag
      361 ttcgtcaagg aatccggaga aggattgcgc ggccgggtcg agccgatacc ggaagtcaag
      421 caattcgagc cctttgctta cagcgcgttc gacctgcccg atcctttcaa gccgcgcacg
      481 acggtcgtcg atagccgcac ccccggcggc gggccgcagc cggacctgaa ccgccgcaag
      541 gaggcgctcg aggcattccc ccttgagagc ctgcagatgg tcggtacgct tgagcagaag
      601 cgggtccaat atgcgttaat caagacgccg gacaagagtc tttaccgtgt caaggccggc
      661 aactacgtcg ggcagaactt cggcgtcatc accactgtca gcgattccgc tgtgaccctc
      721 aaggaggtca tccaggatcc ttcgtcgggt tcttggacag agcgcaccag cagtttgcag
      781 ctgctcgagc aataaacaag gagcatgcga atatgaaacg cgtcggtcgt ttggttcata
      841 tggtcgccgc cccgataggg ctgctactgg tactgagcag tttagcggcg cacggccagg
      901 actccaagac gggcaacggc ggtaccggca acgtcattca gtcgatcgac gtttccacac
      961 aggccgggac ggttaccgtc aggcttggcc tcaaggaggc catgccgaat cctcccgcgg
     1021 ccttcaccgt gaataatccg ccccgggtag cgctcgattt tcccaatacg gcaaacggtc
     1081 tcggcaagag cactcaggac gtcaaccagg gcgacctgcg cacgatacgg ttcggccagt
     1141 cgggagggcg gacgcgggtg gtgttcaacc ttgccaagat gctcaagtac gacaccacgg
     1201 tggaaggcaa taccgtcgtg gtgactctgc agggtggagg cgcaacttcc gccgcggcgg
     1261 cgaccacgca gttcgccgag tccaaatcca gcggcgacca gcacagcgtc caggacgtcg
     1321 atttccgccg cggcaagaat ggcgaggcgc agatcgtcgt cgacctctcc gatacggcca
     1381 ccggtatcga tctgcgccag caaggccgca cggttctggt ggactttctt cagaccatgc
     1441 tgccgccggc gctggagcgc cgcttcgacg tcaccgactt cggcacccag gtcgattttt
     1501 tcgaggttac ccgtcagggc aacaacaccc gcatcaccgt caccccgaag gggcgctggg
     1561 agcagtcggc gtatcagacc gacaagcgct tcatcatcga aatcaagccg gtgatcgagg
     1621 aagccggccg gcctaaggaa ggtaaaggct ataccggcga gaaactgtcg ctgaacttcc
     1681 agaacatcga agtgcgtgcg gtgctgcagg tgatcgcgga tttcaccagc ctcaacatca
     1741 ttaccagcga cacggtgagc ggaaacctga cgttgcgctt gaaagatgtc ccgtgggacc
     1801 aggcgctgga catcattctc caggcgaagg ggctggacat gcgcaagacc ggcaacgtcg
     1861 tctggatcgc gccacgcgac gaacttgcga ccaaggagaa gcttgcgttg gaagcgcggc
     1921 aacagatcgc ggacctcgag ccgctgcgta ccgaaagctt tcagctcaac taccagcgtg
     1981 ccgaggacat tcagaagatc atcacggcgt cggaccagaa gctcctttcc aagcgtggca
     2041 gcgcggtcgt ggatccgcgg accaacacga ttttcgtgca ggatatcgtg gacaagcttg
     2101 atgacgtccg ccggctgctc aagcagatcg acgttgccgt acgccaggtc ctgatcgagg
     2161 cgcgcatcgt cgaggccagc gataccttca gccgcaacct cggggccagg ctgggattcg
     2221 tcgatgcgac cggccgcggc gaacggatcg gcggcacgca ccggatcacc gccggcggcg
     2281 gcttggcgag taccggcgcc ttgaccggtc agatttccgg cacacctgat ttcttcaccg
     2341 acgggctttc caccaacatg ccggcgccgg gtctcggcgg cgtgctgccg gggcaattct
     2401 cgctaatcct gatgaatgac gcgatgtcga aattcctcaa cctcgagatc accgccctgc
     2461 aggcggacgg caagggcaaa atcatctcca gcccgcgggt catcacggcg gacaacgtgg
     2521 aggccactat agagcagggc accgaaattc cgtaccagca ggcgacgagc agcggcgcga
     2581 ccagcgtgtc gttccgtaag gccacgctga gcctgaaggt gaagccgcag atcacgccca
     2641 acgacaacat catcatgaaa ttgcacgtta acaaggacag cgtcggtgcc aacaccagcg
     2701 ccggtccgtc gatcgacacc aagcagattt ccaccgaagt gctggtggat agcggcggca
     2761 cggtcggcat cggcggcatc ttcgaacagg aggagtccag caccaggacc aaggtgccga
     2821 tgctgggcga cataccggtc atcggcatcc tgttccgcca ggacctgcgg cgcaacgata
     2881 agcgcgaact gttgattttc gtgacgccca gggtggtcaa ggacacgctg accgtccgct
     2941 gaacccaacg ctctcccgga acggccgcat cgtttgcggc cgttctgttt ttagcggtct
     3001 gaccgtgcac gaccggtttc gctagaatgg ccgtatgcac gaggcaaccc tggaaaatca
     3061 aatgcaagcg cgggtcgacc tgccgggcag catcttcctg gtcggaatga tgggtgcggg
     3121 caagacctcg gtcggccggg tcctggcgaa acgcctgcag aaaacattct acgactcgga
     3181 tcatgtcatc gaagatcgca ccggcgtgaa gataccggtg atcttcgaga tcgagggcga
     3241 ggccggtttt cgcgtccgcg aatctgcggt ggtggacgaa ctcacggcgc tgtgtgacat
     3301 cgtgcttgcc accggcggcg gtgcggtatt gagcgagaaa aaccgcgaga ggatgaggac
     3361 caggggcatg gtggtgtacc tgcgtgcctc cgtcagagac ctgctcaacc gcactcgcca
     3421 cgataagaac cgcccgcttc tgcaggcgac ggatccgcga gtgcgactca cagaacttta
     3481 cgagaaacgc gatccgctct atcgcgaggt cgcgcacctc accgtcgaca ccggaaacca
     3541 gagccttacc tcgctcgtca accggctgtg tcagctcctg ctcgaaacgc ggtccgagca
     3601 gcggactcag gcgccgtagg tctagcatcc aatgcgaacc ataacggtcg ggctaggcga
     3661 gcgttcctac ccgatttaca tcgacagggg gttgctcgca cgcaccgagc ttctgctgcc
     3721 gcatctgggg cagcgcaaag tcgtgatcgt cactaattcg acgattgccc cgctgtacgt
     3781 ggaaccgctg acctcggcgc tcggcaacgc cgcagtcgag gtcgtgccga tcgtcgttcc
     3841 cgacggcgaa gatcacaaga actggcaaac gctgaatacc attttcgatc tgctgctgac
     3901 gcatcgctgc gaacgcagga ccaccatcat cgcgctgggg ggcggcgtgg tgggtgatct
     3961 cgccggtttt gccgctgcaa cctaccagcg cggcttgcca ttcattcaga tgcctaccac
     4021 tctgctcgcc caggtcgatt cctctgtggg cggcaagacc gcgatcaacc atccgcgcgg
     4081 caaaaatatg atcggcgcct tccaccagcc gcgcctggtg gtcgccgata tcgcgactct
     4141 ggatacgttg ccggcgcgcg agctctcggc aggccttgcc gaagtgatca agtatggttt
     4201 gatcatggat gcccccttcc tgtcgtggct ggaggtcaat atggaaaagc tgcgcgtgca
     4261 cgatgttgac gccctcgcct atgcggttgc ccgttcctgc gagaacaagg ctcgggtggt
     4321 tgcggcggac gagcgcgaag ccggcgcgcg cgcgctgctc aacctcggac ataccttcgg
     4381 ccatgcgatc gaagccggct tgggattcgg tacctggttg cacggcgaag cggttgctgc
     4441 cggaatcatc ctcgccgccc ggctctccga gcgcatgggc ctgctcaact ctgcggaggt
     4501 cgaacgagtg gcccggctca tgagccgggc cggcctgccg accgaggcgc ccgacctggg
     4561 cgtagcccgt tacctggagc tgatgggtca cgacaagaaa gtacaggacg gcaagctgcg
     4621 tctggtgctg ctcagagcgc ttggcgaggc atttgttacg tctgatttcc accccgagga
     4681 cctgcgccag gttcttacgg gagccgcggc gcatgcgtga gctggcagcc tacggctccg
     4741 atcctgcagc ctcgcgcggc cgacgcattg cggagcccgc tccgaccggg cgaacagaat
     4801 ttcagcgcga ccgcgatcgc atcatccatt ccgccgcctt ccgacggctc gagtacaaaa
     4861 cgcaggtttt cgtcaaccat gaaggcgacc tgtttcgcac gcggctgaca cacagtatcg
     4921 aagtggcgca gatcggccgt tcggtggcgc gcaacctcgg gctgaacgag gatctggtcg
     4981 aggcgatttc gctggcgcac gacctcggcc acacgccgtt cggacacgcc ggccaggacg
     5041 cgctcaacga gtgcatgaaa gaatacggtg gcttcgagca caacctgcag tcgttgcggg
     5101 tggtcgacct gctggaacag cgttacggcg cgttcgacgg tctgaatctg tgtttcgaga
     5161 cccgcgaagg catcctcaag cactgctcga tcaagaacgc cgagaagctg ggcggggtcg
     5221 gggagcggtt tctgaagaac cagcggccgt ccatggaggc tcagcttgcc aacctcgccg
     5281 acgagatcgc ctacaacaat cacgacgtcg acgacggcct gcgttccgcg ctgatttcgg
     5341 tcgatcagct gtcctcagtg cgtatctttg cgcgccacct cgatgccgtg cgcggacagt
     5401 acccggaact ggtggaccgg cggctgatcc acgagaccgt tcgtcgcatg atcaatacgc
     5461 tggtctcgga tctgacagag cgaagtcgag ccctgatcgc cgagcatcgt ccggacagcg
     5521 tagaccaggt gcggctggcg ccgccgctga tcgcgttcag tcctgcgatc cgcgaagagc
     5581 agcacgaact caagaaattt ctcaggaaac atctgtatca gcactaccgg gttgcgcgca
     5641 tgagcaacaa ggccaagcgc atcgtccgcg acctgtttca ggccttcatg gccgacccgc
     5701 gtctcctgcc cccggaattc cagtcccgtg ccgaggcgga tctgcccagg gcgatcgccg
     5761 attacgttgc cgggatgacc gaccgctacg cgatccgcga gcaccggcgc ctgttcgccg
     5821 tcgaggagtc ctgaacggcc cgccttaggg ggcaacggcc cggcttttgc cgcgtttatt
     5881 gataggtcgg ctgcaatcgc ggtaacattt acggctcgct tgaatttttg cgcgttccgt
     5941 cgttgtccac ccaggctgcc ggtctggggc gacggcgcgg gcgccaactg aagtagtaca
     6001 gaggtgagac atgaccaatc cggctttgcc tgcgcgacag ggactttacg accccgcaaa
     6061 cgaacacgac gcctgcgggg tggggttcat tgcgcacatc aaaggcgcga agagtcacac
     6121 gatcatcgag cagggcctgc aaattctgaa gaatctgacc caccgcggcg cggtaggtgc
     6181 ggatcccaag gccggcgacg gcgccggcat tcttatccag atcccggacc cgttcttccg
     6241 cgaggaactg gccaagcagg ggatcaaact gccgccgccc ggagaatacg gcgtaggcat
     6301 ggtattcctt ccgcaggaac cggcctcgcg gcttgcttgc gagtacgaga tcgagcgcgc
     6361 catcaaggac gagggtcagg tactgctcgg ctggcgcgac gtaccgcgcg acggttccag
     6421 cctcggcgaa tcggtcaaga gaatcgagcc ggtcatccgc caggtgttta tcggccgtgg
     6481 cgcaggcgtt accgtcaccg acgcgctcga gcgaaagctc tacatcatcc gcaaggtttc
     6541 ggggcacgcc atccaggcgc tcaagctcga gcacggcaaa gagttctacg tgccctcgat
     6601 gtcggcccga accgtccttt acaaaggcat gctgctagcg gggcaggtcg gcagctacta
     6661 caaggacctg caggatgcgc gcgtcgtttc cgcgctggcg ctagtgcacc agcgcttctc
     6721 gaccaataca ttcccgacct gggatctggc gcatccgttc cggatgatcg cccacaacgg
     6781 cgagatcaat accctgcgcg gcaacgtcaa ctggatccgc gcgcgtcagg gcgccatctc
     6841 cagcccgatt ctcgggcgtg atctcgacaa gctctggccg ttgatctatg acggccagtc
     6901 cgactccgct tcgttcgaca acgcactcga gctgctcacc atgggtggct attcgatcgc
     6961 gcacgcgatg atgctgctga ttccggaggc gtgggagaag cacgcccaca tggacccgag
     7021 ccggcgcgcg ttctacgagt accacgcccc gatgatggag ccttgggatg gtccggcggc
     7081 ggtggcgttc accgacggcc gccagattgg cgccacgctc gaccgcaacg gcttgcggcc
     7141 ggcgcgctac ctggtaacca gcgacgacat ggtggtgatg gcttccgaaa ccggcgtgct
     7201 gcccgtgccc gaggagaaga tcatcaagaa gtggcggttg caacccggca agatgttcct
     7261 gatcgatctc gaggcaggcc gcatcatcga cgacaaggaa ctcaaggaca cgctcgccaa
     7321 cgccaagccc tacaaggcgt ggatcgaccg catccgcatc aagctcgacg acctgccggt
     7381 ctccaccaag atagtggagc agagcaaggt gcccttgctt gaccgccagc aggccttcgg
     7441 ttacacgcag gaagacctga agttcatcct cagtccgatg gcacagcagg gtgaagaagc
     7501 ggtcggctcg atgggcaacg acttgccgct tgcggtgctg tcccacaaga acaagacgct
     7561 ctaccactat ttcaagcaat tgttcgccca ggtcactaac ccgccgatcg acccgatccg
     7621 cgaggagctc gtgacctcgc tcgtttcttt catcggaccc aagcccaacc tgcttggcat
     7681 cgacgaatcg aatccgccga tgcgcctcga ggtcagccag ccggtactgg acttcaacca
     7741 gatggagacc atccgacaca tcgattacta caccggcggc aagttcaagt ccttcgagat
     7801 cgacattacc tacccggtcg cctggggcaa ccacgcgatc gaagcaaggc tggccagcct
     7861 gtgcgcacag gccgaggatg cggttcgctc cggctacacc atcctggttg tgtcggatcg
     7921 caaactggac gcaacgcatg tcgcgatccc tgcgctgttg gccctgtccg cgattcacca
     7981 gcacctggtg ggcaagggct tgcgcacctc cgccggcctg gtgatggaaa ccgggtccgc
     8041 gcgcgaggtg catcacttcg cgctgctcgc gggttacggc gcggaagcgg tgcatccctg
     8101 cctagcgatg gagaccattc tccatgcaga cgaatcgatc ccggacgcct accgcggcga
     8161 gaagggagtc gcgaactacg tcaaggcact cggcaagggc ctgatgaagg tcatgtcgaa
     8221 aatgggcatc tccacgtaca tgtcctatac cggagcgcag atattcgagg cggtaggctt
     8281 ggcgaaagtc ctggtggaca agtatttcac cgggaccact tccagcgtcg agggcatcgg
     8341 cgtgttcgag gttgccgagg aggcgatacg gcagcatcgt gccgcgttcg gcgaagaccc
     8401 ggtgctgggc aacgcgctgg atgtgggcgg tgagtacgcc tggcgcgtac gcggcgaaga
     8461 gcacatgtgg acgccggacg cgatcgcaag gctgcagcag tccacacgca gtaattccta
     8521 tactacctac aaggagtacg cgcggatcat caacgatcag tcgaagcggc acatgacgct
     8581 ccgcgggctg ttcgagttca aggtcgacgg gcgcgtaccg gtggcgatcg aggaggtcga
     8641 atccgcggcg gagatcgtca agcgcttcgc caccggcgcc atgtcgctgg gctcgatttc
     8701 cacggaggcg cacaccacgc ttgccatcgc catgaaccgc atcggcggca aatccaacac
     8761 cggcgaaggg ggcgaggact ataggcgcta cgcgccggtg aaggccggcg agagcctgat
     8821 gtcgcggttc ggccgcgaac gcgtggaagt cgacataccg ttggccgcgg gggactccct
     8881 caagtcgaag atcaaacagg ttgcttctgg tcgttttggc gtcactgctg agtatctggt
     8941 gtccgccgag atgctgcaga tcaagatcgc acagggggcg aaacccggcg agggcgggca
     9001 gctgccaggc cgcaaagtgt ccgcgtacat cgccgagctg cgctacgcga cgccgggagt
     9061 cgaactgatc tcgccgccgc cgcaccacga catctactcc atcgaagaca tcgcgcagct
     9121 cattcacgac ctgaaaaacg ccaatcccga tgctgcgatc agcgtaaaac tcgtgtcaga
     9181 agtgggtgtc ggcaccgtgg ccgcgggcgt gtccaaggcg aaggccgatc acgtcactat
     9241 tgccggccat gacggtggca ccggcgcctc cccttggtcg tcaatcatgc acgcgggcac
     9301 cccgtgggaa ctcggactcg ccgagaccca gcagacgttg gtgctcaatc gcctgcgcgg
     9361 ccgcattgca gtccaagtgg acggccagtt gaagactggc cgcgatgtgg tgatcggcgc
     9421 catcctcgga gctgacggat ttggcttctc caccgcgcca ctggtggtcg agggttgcat
     9481 catgatgcgc aaatgccacc tcaacacctg tccggtgggg atagcgacgc aggacccggc
     9541 gctgcgcagg aagttcgaag gcaagccgga gcacgtgatc aattacttct ttttcgtcgc
     9601 cgaggaagcg cgcgaactga tggcgaagct cggcatccgg aaattcaacg atctcatcgg
     9661 ccgcaccgac ctactggaca cgaggaaggg tatcgagcac tggaaggcga gggggctcga
     9721 cttctcgaag atcttctgca ttccgcagat gccggccgat gtcgcgcgct acaacatcga
     9781 gaagcagtac cacggactgg agaaggcgct cgaccaccgc ttgatcgagt tggcgaagcc
     9841 ggcactgcaa cgcggcgaga aggtcacgat cgagatgcct atccgcaaca ttaaccgcac
     9901 cgttggcacc atgctctctg gcgaaatcgc caagcgttat ggccatgacg gcttgccgga
     9961 cgacaccata cacatccgct tcgcgggctc tgcgggacag agcttcgggg cgtttctggc
    10021 gaagggtgtc accctcgacc tcatcggcga taccaatgac tattgcggca agggactgtc
    10081 gggtgggcgc atctcggtcc agccctcgcc aaagttccgc ggcgaaccga ctgagaacat
    10141 catcaccggc aacgtggtgc tctacggggc gatcgctggc gaagcttatt tccgcggtgt
    10201 tgctggcgag cgcttcgccg tcaggaactc gggcgcgcgc gccgtggtcg agggcgttgg
    10261 cgatcatggc tgcgaataca tgaccggcgg cacggtagtc gtgctcggca tgaccgggcg
    10321 caacttcgct gccggcatgt cgggcgggat cgcgtacgtg ctggacgagg acagcagctt
    10381 caagacgcgc tgcaacccgt cgatggtgca gctcgagccg gtattgtccg aagccgagca
    10441 agaggccaag gtaccgcagg atgtatggca catgaatagg gctgacgaga cgattctcaa
    10501 gggcgcgatc gagaagcatg ccggctatac cgcgagcgca cgcgccaggg aaatactttc
    10561 caactgggcc gcttaccgca ccaagttcgt caaggtgttt ccgtccgaat atcgccgcgc
    10621 gctgggcgaa ctggcggcca aacgccgcga gctggcagcg taaaaactca ccactgaggc
    10681 gcagagaaca cgggctcccg tgttctcccg gcgcctctgt gtctctgttg tagggatgca
    10741 agggactgaa gaatggggaa gatcaccggt ttcatggagt accagcgcct cgacgagccg
    10801 cacgagccca aagaggcccg caagaagcac taccgcgagt tctatgcacg cctgtcggac
    10861 gaggcggccg cgactcaggg cgcgcgctgc atggattgcg gcgtcccgtt ctgcatgtcg
    10921 ggctgtccgg tcaacaacat cattccagac tggaacgacc tggtttaccg gcacaactgg
    10981 aagcatgcga tcgatgttct gcactcgacc aacaatttcc cggagttcac cggtcgcatc
    11041 tgccccgcgc cgtgcgagga agcctgcgtg ctgcgcatca acgccgatgc cgtcagcatc
    11101 aagtcgatcg agcacgcgat catcgacaaa gcgtgggaag aaggttgggt aaagccgcaa
    11161 ccggcggcgc acaagaccgg caaaaaaatc gcggtggtcg gctcgggcgg cgcagggctc
    11221 gcttgcgcgc agcagctcgc acgcgccggg cacgaggtca cggtattcga gaaaagcgac
    11281 cgcatcggcg ggctgctgcg ctacggaatc cccgacttca agctcgacaa gagcgtgatc
    11341 gaccggcgcc tagaccagat gcgcgccgag ggtgtcgagt tccgccccag ggtattcgtc
    11401 ggcgtgcgat cccccggcaa gggcgtggtg aacgacgcgc gcgaaaccat cgctcccgag
    11461 aaactgatgg ccgagttcga tgctgtcgtg cttgccggcg gcgccgagca gccgcgcgac
    11521 ctgccgatcc cggggcgcag cctcgacggc gttcatttcg cgatggagtt cctgccgttg
    11581 cagaacaagc gtgttgcggg cgactcgagc gttcccgagc tgtgggggac caacaagcat
    11641 gtcgttatca tcggcggcgg cgacaccggc tcggactgcg tcggcacctc caaccgccac
    11701 ggcgcgcgct cggtgacgca gttcgaactg ctaccgatgc cacccgatgt cgagcgcaat
    11761 ccggtgtggc cgttctggcc gacgcggctg cgcacctcct cctcgcacga ggagggcgta
    11821 aaccgcgact ggtcggtcgc gaccaaggaa tttattggaa gcaacaatcg cgttaccgcc
    11881 ctgagggcgg ttcggctgga atggaaagaa ggcaaaccgc acgaggtgcc gggctcgcag
    11941 atcgatatgc ctgccgatct ggtgctgctg gcgatgggct tcgtttcgcc cttgcagtcg
    12001 atgctcgagg aattcggcgt cgaaaaggac gctcgcagta acgtcaaggc cgacaccgag
    12061 ggcccgaagg cctacgcgac gtcggtacca aaggtgtttg ccgcggggga catgcgtcgc
    12121 ggccagtcgc tggtggtgtg ggcgatccgc gaaggacgcc agtgcgcgcg cgcagtggac
    12181 gagttcctga tgggcgactc cgtgttgccg cgctgaaaga tctacttcgt ccccgccctg
    12241 tcgacggcgt ggagagtgcg ccgagtatgt tggtgccgat atcttgactg acgcggtttc
    12301 accaccctct ccgcctgagt accaacagga ccgggtactc agtgcatgtc gaaagtacga
    12361 aaatcctttt ggtagtcagg gggagaggga cggggtgagg tggatgtcca agctcaagaa
    12421 cgatacgttc atccgcgcac tgctgcgcga gtccacgccg tatacgccgg tctggatcat
    12481 gcgacaggcc ggtcgctatc ttccggagta caaccaggcc agaacgcgtg caggcagctt
    12541 cctcgacctg tgcaagaacc cggacctggc caccgaagtc acgctgcagc cgttggcgcg
    12601 cttccccctc gacgccgcca tcctgttctc cgacatcctc accatccccg atgcgatggg
    12661 actgggattg tcctttgccg aaggcgaggg gccgcgtttc gagcggccgc ttcgcgacga
    12721 acggcaaatc cgcaatctaa gggttcccga ccccgacatg cacttgcgct atgtgctgga
    12781 tgccgtgtcc cagatccgca gggctctcgc cggtgaagtc ccgctgatcg gcttctcggg
    12841 cagccccttt acgctggcct gctacatgat cgagggtcgc ggagagtcgg attttcatga
    12901 gaccaagcgc atgctctacc agcgcccgga actgctgcac catgtgctcg acgttaatgc
    12961 gcgcgccgtc gcggcctacc tgaatgcgca gatcgagtcg ggtgcgcagg cggtgatgct
    13021 gttcgatact tggggcgggg cgctttccgg cgaggcgttt cacgaattct cgctgtgcta
    13081 catcgagcat gtgttcgcac aactgcggcg cgagcatgac ggcgagcagg tgccgcgcat
    13141 cgtcttcacc aaaggcggcg ggcagtggct ggaaagcttg gcgcactgcg gtgctgatgc
    13201 ggtgggcctg gactggacgg tggacatcgg caaagcccgg gcccgagtag gcgatcgcgt
    13261 ggcgttgcag ggaaaccttg atccggcggt gctgctgtcc gcgccggaag tcatccgccg
    13321 cgaagtcggc aagattcttg cgggcttcgg ccatggcaac ggtcatgtgt tcaatctggg
    13381 ccacggggtg tcccagttca cgcctccgga gcacgtcgcg gcgctggtcg aaacggtgca
    13441 ctctggaagc ccgcaatttc actaggttta cgagggggtg ccctgccgaa ccccagtatt
    13501 tatgcacaat tcggcagggt cccctgcgaa aacgcaggcg cagtaaggct ttccgcgagg
    13561 ctgttatata agtttttgaa aaatatgtgg aattatgagg ttgcagcgcg aatcccgcgc
    13621 ccgatctagg tttcagcggg atttcagacg aggcgcgccg ccggtttaat caacagattt
    13681 atccacaacc gttccgtgct cggaaaaagc ccttccgcgc aagtgattta ggtgcgtaac
    13741 cgcagacagt acgcgagagg ccgccagtaa acttctgctc ggtgctttgc gtagcggatc
    13801 tcaatgacca tcgccaggat tgcactcgac gtcccgctag agacgctttt cgattaccgt
    13861 acgggggatc tcgcaccggt tcccggtcag ctcgtggtcg tacccttcgg caggcgcaga
    13921 gacgtgggcg tggtcgtgga actcgccgat cgttccgaga ttgccgatgc gcgcctgcgc
    13981 cgtattgagc gcttcctccc gcttgatccg ttgccggagg atctgctggc actgacccgg
    14041 ttctgcagcg actactatca tcaccctttc ggtcgggcct tgttgtccac gttgccgtcg
    14101 ctcatcaagc gcgccggcta cagcggaccg aagcgccaat ggcagtatgc gctcacctct
    14161 gcggggcgcg cgctgcgtgc agatcaattt ccgcccggcg cagcggtaaa gcgtaggctg
    14221 ctggcagcgt tgtcgacgtc ggcagcgctt gggcaggcgc aggtgcgagc gctttcggcc
    14281 cgggcaccgg cgttgctgca gcagtggatg gaggcgggat gggtcgagaa acgccccgcc
    14341 acaataccgg atgcgccggt gtcccgatct gagcagggtc cccaactgac cggcgagcag
    14401 cgccgcgcag tcgagatgat ctcggctacc ttcggaacgt tctctccctg gttgcttgac
    14461 ggcgtcaccg gcagcggcaa gaccgaggtg tactttcgcc tgatcgaggc tgccatcgtg
    14521 cacggttcgc aatctctgtt gatggttccc gagatcaatc tcacgccgca actagaggcg
    14581 cggtttctac agcgtttccc gggagtaagg ctggtcagcc tgcatagcgg gctgtcggac
    14641 ggtgaacgct gtgagcgttg gttggcggcg tttgcgggcg acacgcaggt cgtgctcggg
    14701 acgcgattgt cggtgtttac gccgctaccg cgactggggc tggtaatcgt ggacgaggag
    14761 cacgatgctt ccttcaagca acaggaaggg ctgcgttact cggcgcgcga tgttgctctt
    14821 ttcatggcgc ggcaacgccg cgtgccgatc gtgctgggct ccgccacgcc agcccttgag
    14881 agctaccaca acgcccgcag cgggcgattt ggccatctgc acctgagtac tcgtcctgcc
    14941 gccagccccc cgcaaattcg gctgatcgaa acccgcgcag cacggcagac gcacgggatg
    15001 tcggaaccgc tgttgcaggc aatacgcgaa cgcctgttgc gcggcgagca gagcctggtg
    15061 ttcctgaatc gccgcggctt cgcgccggcg ctggtgtgcg gcgcctgcgg ctgggttgcg
    15121 cagtgcgggc gttgcgctgc gcgtctggta tggcatctgc gcaaagaacg gttgcgatgt
    15181 cactactgcg gacacgaaga acacctgtcg caggtgtgcc cggactgcgg caatcaggat
    15241 ctgaagggct tggggcaggg tacccagcgt ctcgaggaag cgcttatcgg aattttcccc
    15301 ggcgcgcgca tcctgcgcat cgaccgcgac agcacccgcc gcaagcgctc gtggcaggcg
    15361 atgcgcgagg atatccacgg cgaccgcgtc gacattctgg tcgggaccca gatgctcgcc
    15421 aaggggcacg attttccgaa actgacgctg gttgccgtcg tcaatccgga cagcgcgttg
    15481 ttttccgccg atttccgtgc agccgagaag ctgttccagc agttgatgca ggtgaccgga
    15541 cgaaccggcc gcgccgacct gtcgggcgag gcgctggtgc aaacgcagtt ccccgcacat
    15601 ccgttgtatc aggcgctgtt gctccaggat taccgggcct tcgcggacga attgcttgcg
    15661 gaacgcaagc acagcggctt tccgccgttc gtgtatcagg cgctgttgcg cgccgaggca
    15721 ctcagcgagc gcgcgttgat ggattttctg acggaggcag ttcatcgtgc tgcaggtcat
    15781 gacgaggatg tgacgcttta tgacccggtg ccggcggcga tcgcgcgggt tgccgggcac
    15841 catcgcgccc atttactcgt gcagtcctcc tcacgcggcg cgctgcagcg cttcctgtcg
    15901 aactgggttc cggcgctcgc gcagaagaag gcgggccggg tgcgctggtc gctcgacgtc
    15961 gacccgctcg agttgtgaga agtccggcgt gtccgggccg gtataatcgc acttttcccc
    16021 gacgccccac gcaatgagca acgcccccgc gctggatctg cgctcccatt tcaccgggct
    16081 gttgcgatct gcccttgtcg agattgcgcc cgacgcagcc aatctgccgc tgattctgga
    16141 gcgccccaag tccgcgcagc acggcgacta cgcctgcagc gtggcgatgc agctcgccaa
    16201 gccgatgaag cgcaacccgc gcgacatcgc gcaggcgctg gtgaaggcgc tgccgcaatc
    16261 gccatacctg gagaaggcgg agatcgccgg tgccggcttc gtcaatctct atctcaagcc
    16321 tgccgccaag caacagatct tgcgtctggt gctgcaacag cgccacgcct acggacgcgt
    16381 cgatgtcggt ggcggcagaa aagtgcaggt ggaattcgtt tccgcgaatc cgacgggccc
    16441 gctgcacgtg ggccacggcc gaggcgccgc atacggcgcc agcctcgcca gtctcctgga
    16501 agcggctggt ttcgccgtca cgcgagagta ctacgtcaac gacgctggcc ggcagatgga
    16561 catcctggcg ctatcgacct ggctgcgcta tgtggagatt gccggcgcgc ggttggagtt
    16621 tccggcgaat gcctaccagg gcgattacgt gcgcgacatg gcgcggcaga tcttcgaggc
    16681 gcatggcaaa cgctacctcc accccgtcga gtcgatgatg cgcgatgctc cgccggcgca
    16741 gagcgacccg gaagcgcgcc tcgactacct gatcgccagt gccaagaggg tgctcggccc
    16801 cgactacgat tacgtgcacg atttcgtgct cgccgagcaa ctcggcgatt gccgcaagga
    16861 tctccaggaa ttcggtgtga ccttcgactc ctggttctcg gaaaaatcgt tgttcgactc
    16921 aggcttggtg gtgcgcgcag tcgagcaact tcaggcaagc gggcacgtct accggaagga
    16981 cggcgcaaga tggtttcgct ccactgtttt cggcgacgaa aaggaccgcg tggtccagcg
    17041 cgacaacggc cagttcacct actttgcgtc cgacatcgcc tatcacctca acaagtttca
    17101 gcgtggcttc gacctgctgg tcaacgtctg gggtgccgac catcacggtt acattgcgcg
    17161 cgtgaagggt gcgctgcagg cgttgaggct cgaccccgcg caactcacca ttgcgctggt
    17221 acaattcgcg gtactctacc gtgacggcaa gaaagtctcg atgtccacac gcgccggcga
    17281 attcgtgacc ttgcgcgagt tgcgtagtga ggtcggcaac gacgcagcga gatttttcta
    17341 cgtgctgcgc aaaagcgatc agcatctcga tttcgacctc gatctggcca aatcgcagag
    17401 caacgacaat ccggtgtact acatccagta tgcccacgcg cgcgtatgct cggtcctcga
    17461 gcaatggggg ggcgacgcat ccacgctggt caacgccgat ccggggccgc tcgttggtga
    17521 acaggagctc gaggtcctgc agaagctgtt ggattacccg gagacgatcg agagcgcggc
    17581 acgagacctc gcaccgcacc agttggcctt ttacctgaag gaactggccg ccgcgttcca
    17641 cagctactac aactcgaccc gcgtgctggt cgatgacgag agcatccgac tggcgcgcct
    17701 cgcgctggcc aacgcagtga gacaggtact ggccaacggt cttgcaatcc tcggggtgag
    17761 cgcccctgag aagatgtgag cttgcgttcg aatctctgag gtaaacttcg atttcatgag
    17821 ccgcgactac aagaacagcg ggtctgctgc gcccaacggc cgcggcaatc cgatgggggt
    17881 cgggatcctg atcgggctat tgctcggtct gtgcattgcg ctcggcgtgg cgctgtacat
    17941 caacaagggc gccaatccct tcgtgcagaa agcgaagccc gtcgaggtta ctggacggga
    18001 aacgccggcc acggatgcac ccaagttgcc gccccccgcc gacaagcccg ccaagaccgc
    18061 ggaggccaaa ccacgctttg atttttacaa gatactgccg ggcaccgagg aagcggtaac
    18121 cgacaaggaa ttcaagcgca cgccgccggc ctcgagcaag gaggtctact ttctccaggt
    18181 ggcggcgttc cagaacccgg cggatgcgga caatctcaaa gcgcggcttg cgctcgccgg
    18241 gatcgaggcg cagatccaga ccgccacgct gcccgacggt aaggtgtggc accgggtccg
    18301 ggtgggtccg ttcccgaaca aggatgaact gggcaagtcc cgcgccgccc tgaaagaaaa
    18361 caatctggaa gcgaacctga tcaaggttcg cgaaccgaca cagtaatctc caaaccgcta
    18421 cgggaatatc catgaaccgt tttcacacag cgctgatggt cgcgtccgct tttctctgcc
    18481 tggtattcgc tccggccggg ctcgccaggc cgactcccga cgtagactac aaagcgatca
    18541 ctcctccgca gccggtcgca ggcaagggag tcgaggtgct ggagtttttc aactatgcct
    18601 gctcgcactg ttacgacttc gagcccctgc tgaaattgtg gctgaagacc aagcccaagg
    18661 acgtggagtt ccgctacgtg ccggcggtgt tcaatgagcg catgttgccg ctggcgaaga
    18721 cttattacgc ccttgaagag ttggagctgc tcgacaggat gcacgaaagg gtctacgagg
    18781 caattcacga gaagcacgtc aatcttgccg accggggcgt gatgatgaag tggatcgccg
    18841 agcagggcgt ggatgccagg aagttcgagg ctacttacga ctcctttagc gtggacagca
    18901 aggcgcagcg tgccgggcaa ctcactcgca gttaccgcat taccgccaca ccctacgtcg
    18961 cggtcgcagg caagtttctg accgcccctt cgctgacgct gcgtgcggac ggaaacatcg
    19021 atcacaaccg ctttttccag gtactcaacg acctggttat catggggcgg ggcggcgcca
    19081 aataggcccg cggcaggcat tcccgtgggg cgctttactg ccgcgctgag tttcgcatta
    19141 atctttgcag cgttcggcgt aactgcttcg gcagagccga tggaggacgt ggattacgtt
    19201 ctgatcccgc ttcagccggg cgcaagcggc gggcgcatcg aagtcattga attcttttat
    19261 tacggttgtg attcctgcta tcgcttcgag ccgatgcttt ctgtctggct caatgagccg
    19321 gcgcccgacg tttctttcag gcgggtgccg gcattgcggc gcatggaatg ggtgcccctg
    19381 accagactgt acttcgcact cgaagaagct ggcgcactcg atcgactgca tggtgaggtc
    19441 tacaaggcga tccacgagaa gggcatcaac ctcggtgatg cgggcgaact gctcaagtgg
    19501 gcggaaatga acggactgga gcgcgccagt ttcgggaaga ttctgaattc cgaaagcatg
    19561 agcgcgaagg tgcagaaggc acgcgacacg accgtcgcgt acggaatccg cgccacaccg
    19621 tccatcgtgg tggacggaaa gtatctgacc agcggcggca tgctcggcag cctcgactcg
    19681 ctgttgccgg tggtggacgg cctgatcgac aaagcccgca gggaacgcgc ggtgaaatga
    19741 ccgaagcgcc gctcagagtc gtcatcaccg gcgcttcgag cggaatcggc aacgcgctgg
    19801 cacacgagta cgccaaacac ggcgccaccc ttggattgat cgcgcgccgg ggagatttgc
    19861 tcacccggct cgcgggctcg ctcccggtgc gctcctacac gtataccgca gacgttcgcg
    19921 atgcgcgggc tttggcagtc gcggcgaacg acttcatcgg gcgagttggc tgcccggacg
    19981 tggtcatcgc caacgccggc gtgagtgccg gtacgctgac cggtaatccg caggacaacc
    20041 aggtattcga agagatcctg gcaatcaatg tcaccggaat gatgctcacc tttcagcctt
    20101 tcatcgaacc gatgaaggcg tgcgaacacg gcacgctggc tggaatcgcg agtatcgccg
    20161 gattccgcgg cttgcccgga gcggcagcgt attcggcctc gaaagctgcg gcgatttcct
    20221 atctcgagag cctgcgcgtc gagctaaaga gaagcggcat ttcggtggtg accatctgtc
    20281 cgggttacat cgccacgccg atgacgggca gcaatccata ccggatgccg ttcctgatgg
    20341 atgcggatgt ggcggcaagg aaaatcgccg gtgcgatcgc acggcggagg cgcctctacg
    20401 tgctgccgtg gcagatggcg gtggccggat ggttcatgcg gcgattgcca cgcccgctct
    20461 acgattctct gttcagtcgc gcgccgcaca agccgcgccg cagcgtcact taggccacga
    20521 gcgcactcag cggagcacga tccccagcgc cgtttccagc tccgcgagcg cggttgccgg
    20581 atcttcgact ttgatggtgc gcatgcccat tgccttcgcg ggcttgaggt tggagcccag
    20641 gtcgtcgaga tagacggctt gttcgggagc gatgcggagc tgcgtgcaca ccagctcgaa
    20701 aaaacgcggc tcgggtttgc gtgcgccgag cttagaggat tcgatcaccg tatcgaacag
    20761 gtcgagcacc gtctggcatt gcgcgccacg ttgcgggtcg cggtcgaacc cgtggcctgg
    20821 cccggcgttg acgttattgg ttacacaggc gttggtgtaa tgctgtttgc acgcccggag
    20881 cgcacgcacc atctccgtcc gcacttcccc atagagcagg tcgatgacat cgagtccgcg
    20941 tacttcgtgt cccgcggcgc gcgtttccgc tgcgtacgtc cgatcgaact ccccgggggt
    21001 gagttcggaa cgctcgaagc gggcccaggc gttaccgtcg gaattgcggg cattgatttc
    21061 gcgcaggaag tgcgccggca gcccacgagc ctgctcgaaa cgacggaagg cgacaaaagg
    21121 actttcggtc atcacgccgc cgaagtccca gagcacggcg cgcaccgccg ggtttggcat
    21181 ccgcgcccta cttcttgttc ttcttgtagg tgtccaccgc gcgcttgagt tctttgtact
    21241 cttcgcacgg gataggcgtg cacagcttct gcaactcggc gagatgctgc tcggccttgg
    21301 gtaggttgtc ggccaacaga taggcctcgc cgatgtactc atgtgcatgc ttgtgcttgg
    21361 gattgagttt gagcgcttcg ttgtagtggt tgaaggacgc atcgaatttg ccagccttgc
    21421 gataagcgta gccgaggaag ttgtgcgcat ccgcattctt ggggtcggat tgaaccgctt
    21481 tcgagagcag ttcgaccgcc ttattccaat cctgcgattc cacggctttt ttgccttcct
    21541 ggaaattcgg gtcgtcgctc gcggccgcgt catcgtcgcc caggttggct gccgccaggg
    21601 gaacgaacgc gaccaggagc gccgccagca gcacaccgaa gagcgatttc aatgggttct
    21661 tcattctctc ctccttccgg cccggcaacg gggctagcta cggcattatt tataacgtat
    21721 cggcagccgg tttgggaggc tgcagatgcg tttttattcg actgtcactg acttcgccag
    21781 attcctcggt ttgtccacgt cggtgtcctt ggcgagcgcc gcatgatagg caagaagctg
    21841 caggggcacg gtgtgcagga tcgggctcag atatcctgcg tgcccggtca tgcggatgac
    21901 gtggatgccg tcgccggcct ggatgcggct gtcggcgtcg gcgaatacgt acagctcgcc
    21961 acctcgggcg cgaacttcct gcaggttgga tttgagcttc tccagcaacg catcattggg
    22021 cgcgatcgcg atcaccggca tgtccttgtc caccagcgcc agcgggccat gcttgagttc
    22081 tccggccgga taggcctcgg catggatgta ggatatctcc ttgagtttga gcgcgccttc
    22141 catcgcgatc gggtagtgca tgccgcgacc gagaaaaagc gcatgctgct tgccggaaaa
    22201 gcgtttggcc cacttcgtta cttccggctc gagcgtcaga acctgctgca aagccgacgg
    22261 caggtggcgc agcgcatgca gatgctgctt ttcctgctcc gccgacagcc gcccgcgcag
    22321 cttagcaagc accagcgtca acaggaacag cgccgccagc tgggtagtga acgccttggt
    22381 cgaagccacc ccgatctctg ggccggcgcg ggtgagaaat ttgaggctgg cgaggcgaat
    22441 cagcgtactt tccgcaacat tgcagatcgc cagactcagg ttctggccga gggattttgc
    22501 gtactgtagc gcggcaatgg tgtcggctgt ctcccccgac tgcgagacgg tgacgatcaa
    22561 cgcatccggg ttgggtacgc tttcgcggta gcggtactcg ctggcgattt ccaccgagca
    22621 gggtatgccc gcgattgcct cgatccagta acgcgccacc aggcccgcat ggtggctggt
    22681 gccacaggcg agcagcgtga ccgcgttgac gcgcttcatc atggtttcgg cctccacccc
    22741 aaacagctgc ggcgcgatcg attgtgcgcc ggtcaccatt tccagggtgt tggccaccgc
    22801 acccggctgc tcgaaaatct ccttctgcat gtagtggcgg tacggcccca gttcgaccgc
    22861 gtcggcggag agcttgctct cgtggatggc acggtcggtt tctcgaccat gtgcgtcgaa
    22921 aacgtgatgg ccgtctactt tgagctccgc cacgtcacct tcctccagat agacgattct
    22981 cttggtgacc tgcaacagcg ccgaggcatc cgacgccaca aaattctcgc ccttgcccaa
    23041 tcccagcaga agcggcgcgc ccttgcgtgc cgccaccaac gtgcccggat gcttctcgga
    23101 cagcaccgca attgcgtagg caccctggag ttcgacggcg gacttgcgca ctgccgcgaa
    23161 cagaccacct cccctcctga gattgaggtg gatcagatgc gcaacgactt cggtgtcggt
    23221 gtcggagacg aatttgtagc cggccgcgct gagtcgctct cggatctgtt cgtggttctc
    23281 gatgattccg ttgtggacca ccgccagtcc gtcctcgctg acgtgcggat gcgcattgcg
    23341 ttcggtcggc gcgccatggg tggcccagcg ggtgtgggcg atgcccaggc taccatgcac
    23401 cttggcctgt tccgccattt cggcgagcgc cgccacccgg ccaacgctgc gcagccggtg
    23461 catgccgccg ttgacgaccg cgagccccgc cgaatcgtaa ccgcggtatt cgagcttgcg
    23521 caggccttcg agcaggatgg gaacgacgtt acgccgcgct acggctccta cgattccgca
    23581 catgctagtt cggtaatgag tgacgagtaa tgagtgatgg gtagaaacgc ctcactggag
    23641 agccgccgct ctcacaggga aatgcctgca gcgcattact catcactctt taccgtcttt
    23701 ttgggtcgct tccagccggg caccgaaacc tgcttcgagc gcgacagcgt cagttcgcct
    23761 ggcggggtgt ccctggtaat ggtgctgccg gcaccgatgg tgctgccgcg gccgacggta
    23821 acgggtgcga ctaattgtgt gtccgagccg acgaacacat cgtcctcgat caccgtgcga
    23881 tgcttgttgg cgccgtcgta gttgcaggtg atggtgccgg cgccgatatt gacgttgcgt
    23941 ccgacggtgg aatcgcccag ataggtgagg tgattggcct tggacttgtg gccaagtttc
    24001 gtcgccttga tctcgacgaa attgccgacg tggacctcgt cggccagttg cgtgcccggg
    24061 cgcagccggg cataggggcc gattctgcaa tgcctgccga ccacgacgcg gtcgaggtgc
    24121 gagtaagggg cgatcacggt gccggcgccg atctcggcgt ctttcaacac gcagccggcg
    24181 cccactttcg cgtcatcggc cagcgacacc ttgccctcga aaatgcagtt cacatcgatg
    24241 gaaacatcgt ggccgcacac caactcgccg cgcaagtcga aacgccacgg atcggccagc
    24301 gttacgcctt tgcgcatcag ttcgcgcgcg cattccaact gatagacccg ctccaggtag
    24361 gcgagttgtt ccttgccgtt gacgccgaga atttcccagg tctcggccgg atacaccgag
    24421 gttaccggtg tcctcgaggc aatggccagc gacacgatgt cggtaaggta gtactctctc
    24481 tgcgcattgc gattggtgag cctggccagc cattcaccga gcctggcgct tgccgcgcac
    24541 agcatgccgg tgttgacctc gcggatcttc ttttctttcg cgctcgcgtc tttttcctcg
    24601 acgatgcgct caatcgcgcc gtctgcgtcg cgcacgatgc ggccgtaacc ggtcggatcg
    24661 tcgagcagcg cggtgagcag cgacagctcc gcgggatttg atgccagtcg cgccaacgtt
    24721 tcgtttcgtg tgagcggcac gtcgccgtaa agaatcaggg tggacccgcc gggcaacaaa
    24781 tgcggcagcg cctgttgcac tgcgtgaccg gtaccgagtt gcggttcctg caggacgaac
    24841 ttcagcacgg gatcggcgaa tgctgcaggc acagcatcgc cgccgtggcc gtaaacgacg
    24901 atggtgcggt cggcgtgcag cgcccgggcg cggtccagta cgtgagcaag cagcggctta
    24961 ccgccgagcg cgtgcaagac cttcggcaat cgggaataca tgcgtgtgcc cttgccggca
    25021 gcgaggacca cgacgttgag gggggcgctt ttcacagggg gcacggaaac cgcagaggat
    25081 tatcgcataa cgccccgcaa agacgatgct gccgctaatt gacgctgatg ccttgctcga
    25141 taagcagcgt tctgatgtcg ttcagcacca cctggggcaa caaaaaggaa gtgctgtaaa
    25201 tctcccggat cgaaccgcgc cggtcgatca ggaacacctt gagtacgtgc gacagcgacc
    25261 cggtcgactt gccgttttcg tcgagcacca cggacaggtc ctggccgaat ccttccagca
    25321 tgggccgcag ccccaccggt gaaagcgtgg tcaggaagta ccagggcagc ccgcccgtgt
    25381 cggccatatg gctgcccccg tacaggcgca tggcctccgg cgtatcgtgt tcggggtcga
    25441 acgacaggct gacaaaacgc aagtgcggca gcagcgcggt ttcgtgatcg agcgactttt
    25501 tcagttcttg cattacctgg tacgcgaaag gacagcccca ggcatcgctg caactggtgt
    25561 acatgaacga cagtaccgtg actttgccgg tggtgaagcg cgtaagcggc ttgggttggt
    25621 cgtcggtatc gagcactgtt ccgtccggtg cggtcatgat gtgctcgagc cggtaggagc
    25681 cagcgggcgg cggcacgaat tcgagttggc gggtcggcac cccatcgtca ccggcagcaa
    25741 acgcggcggc ggccagaaac aggccgccgc acaatgcaca gggacgcagc atcagcgtgc
    25801 ggccgcaatc tcgccgggcg gctccgcgtc cccaaacgcg gatttcagcg acttcgag
//
LOCUS       DASQOP010000177         5870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1482530, whole genome shotgun sequence.
ACCESSION   DASQOP010000177 DASQOP010000000
VERSION     DASQOP010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5870
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482530"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VE030_07245"
     CDS             complement(<1..564)
                     /locus_tag="VE030_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HZF23243.1"
                     /translation="MSKRNAPPFRADHVGSFLRPQGLLDARDRFKQSALSKAELRAVE
                     DGAIRDIVKFQEDLGLQGVTDGEFRRTYFHIDFLEQLDGVETRGGIAASFHGKAGAVN
                     FAPPVMQVTGKLRHARPIQTEDFKFLKSVTKRMPKVTIPSPTMLHFRGGRAAISKEAY
                     PDMDAFYDDVARCYRDELKQLGTEGCTY"
     gene            736..1890
                     /locus_tag="VE030_07250"
     CDS             736..1890
                     /locus_tag="VE030_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZF23244.1"
                     /translation="MFGMTVIVAGAFAVAPVHAEEPIKVGMVLEMSGPFADIGRQILD
                     GARAYMKANGDTVAGRRIELIVRDTTGAAPEIAKRQAQQLIANDRVDFLAGFGLTPNA
                     LAAAPVATTGKTPMIVMNAATSIITTKSPYIARVSFTLPQITAPMATWAIKNGIKEVY
                     TLVSDYGPGIDAETQFRKTFEAAGGKIAGDVRVPLRNPDFSPFLQRIKDAKPQAVFIF
                     LPAGEQPVAFMKGFNERELDRAGIRLIATGDVTEDGTIEAMGDTPLGVVTSHHYSVAH
                     DSAQNKAFLKAFADLDNAHRPNFMAVGGWDGMTAIYEVVRRLNGKLDGDKAMEVLKGL
                     KLDSPRGPVTIDSETRDAIQTIYIRRVQKVNGKLYNVEFEQVPNFKDPGK"
     gene            2141..3772
                     /locus_tag="VE030_07255"
     CDS             2141..3772
                     /locus_tag="VE030_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HZF23245.1"
                     /translation="MSDISREVLAERVEQRRTFAIISHPDAGKTTLTEKLLLFAGAIH
                     IAGSVKARKATRYATSDWMEIEKQRGISVASSVMQMEYRGKVVNLLDTPGHQDFSEDT
                     YRVLTAVDAALMVIDAGNGVEAQTLRLLEVCRSRGTPILTFINKMDREVREPLDLTDE
                     IERVLGMAAVPFSWPIGMGKTFHGVYDLAGDRIRVFRAGDDRLGQADEVISGLDHPSL
                     RERFGTEFERARHDIDLIRMATPAFSREEFLAGRQTPVFFGSAINNFGVREVLDALVD
                     IAPPPQPRVAMQGKVDPHGKNFSGVVFKIQANMDPAHRDRVVFVRVCSGRFERGMRVK
                     IQRTGKEIRPNNVVSFLSQRRELLDEAYAGDIIGIPTHGGIQLGDTLTDGAELQYAGL
                     PYFAPEIFHTIEVLNPLRAKQLRTGLMQLGEEGAIQVFRPLRNSMMLLGAVGTLQFDV
                     AAHRLQHEYGVEARLMPASYTCARWVSADDPAELERFVEANAGRLARDAADALAVLTN
                     TKYEMKVVQDRWPNIRFHARREHAGLVLDRIANPA"
     gene            4107..4355
                     /locus_tag="VE030_07260"
     CDS             4107..4355
                     /locus_tag="VE030_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003213503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23246.1"
                     /translation="MDVHRRLVSAAIATAAAALFATGVVGTATAQEVKVKCYGANACK
                     GKSECKTSMSGCKGKNGCKGQGFEQMTEKACVEKLGRT"
     gene            4573..5151
                     /locus_tag="VE030_07265"
     CDS             4573..5151
                     /locus_tag="VE030_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZF23247.1"
                     /translation="MEQRAEFKSMKESTKADWDIIVARYQPFAKELPSRILAHLRLLG
                     GDSGGFAVDRLQHSLLTATLAHKAGEDEEYVVCALLHDVGDTLGSWNHPEVSAAVLKP
                     FVSEANHWMVEKHGIFQGYFFFQHVGMDRNMREQFRGHPHFERTARFCEIYDNPAFDP
                     GMECAPLEFFEPMLRNVFSFPKNSIYKNTCQT"
     gene            5213..5476
                     /locus_tag="VE030_07270"
     CDS             5213..5476
                     /locus_tag="VE030_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HZF23248.1"
                     /translation="MAGRCKHLSEVRDVTPSAQGCEDCLKTGDTWLHLRMCLSCGHVG
                     CCDSSKNKHATKHSHTTSHPVMKSFQPGEHWGWCYVDEIYLQF"
     gene            complement(5597..>5870)
                     /locus_tag="VE030_07275"
     CDS             complement(5597..>5870)
                     /locus_tag="VE030_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23249.1"
                     /translation="LHPKIALEIVKRYATTEKMQTRVMLAAKRSIQLLHNALLTGYQA
                     YSPTQIRKGRAVGDKRGQDRRLAYQAVGFPDRRFNERRGRSRRAVA"
BASE COUNT         1145 a         1802 c         1842 g         1081 t
ORIGIN      
        1 gtaggtgcag ccctccgtcc cgagttgctt gagctcatcg cgataacacc gggccacatc
       61 gtcgtagaag gcgtccatat ccgggtaggc ctccttgctg attgcggcgc gcccgccgcg
      121 gaaatgcagc atggtgggcg acggaatcgt cactttcggc atgcgcttcg tgaccgactt
      181 gaggaacttg aagtcctcag tctggatcgg ccgtgcatgc cgaagcttgc cggtaacctg
      241 catcaccggc ggggcgaagt tcaccgcgcc ggccttgccg tggaagctcg ctgcgatccc
      301 cccgcgcgtc tcgacgccgt ccagttgctc cagaaagtcg atatgaaagt aggtgcgcct
      361 gaattcgccg tcggtgacgc cctgcagacc gaggtcctcc tggaacttca cgatgtcgcg
      421 gatcgcgccg tcctcgaccg cgcgcagctc cgccttcgag agggcgctct gtttgaaccg
      481 gtcgcgggcg tcgagcagcc cctggggccg caggaaactg ccgacgtgat ccgcccggaa
      541 cggcggcgcg ttgcgtttgc tcatgatgcc ccttccggat cgctcgattt atgcaggcat
      601 ttcaggtcat cgcggggtgc ggtgtcaacc gcaagttggg tgatacgata cttgttatcc
      661 cagtcttcgc catcgtgatg cgaagcacaa atcgcgcagt cggaggagaa agcgatgtca
      721 acaggaaaac ggggcatgtt cggcatgacg gtcatcgtgg ccggcgcctt tgcggtcgcg
      781 ccggttcacg ccgaggagcc gatcaaggtc ggcatggtgc tggaaatgag cggccccttt
      841 gccgacatcg gtcgccagat cctcgacggc gcgcgggcct atatgaaggc gaacggggac
      901 acggtcgccg gccgcaggat cgagctgatc gtcagggaca ccaccggcgc cgcgccggaa
      961 atcgccaagc gccaggccca gcagctgatc gccaacgaca gagtggactt tctcgcgggt
     1021 ttcggcctga cgccgaacgc gctcgccgcc gcgccggtgg cgaccacggg aaaaacgccg
     1081 atgatcgtga tgaacgcggc gacctcgatc atcaccacga aatcacccta tatcgcacgc
     1141 gtttccttca ccctgccgca gatcactgcg ccgatggcga cctgggcgat caagaacggc
     1201 atcaaggagg tctataccct ggtgtcggac tacggcccgg gcatcgatgc cgagacacag
     1261 ttcaggaaga cctttgaagc agcgggtgga aagattgccg gggatgtgcg cgtgccgctg
     1321 cgcaatccgg atttttcgcc cttcctgcag cggatcaagg acgcgaagcc gcaggcggtg
     1381 ttcatcttcc tgccggcagg cgagcagcct gtggctttca tgaagggctt taacgagcgg
     1441 gagctggaca gggcgggcat ccggctgatc gcaaccggcg acgtcaccga agacggaacg
     1501 atcgaagcga tgggtgacac gccgctcggc gtagtgactt cgcatcacta ttccgtggcc
     1561 catgacagcg cgcagaacaa ggcatttctc aaggctttcg ccgatctgga caacgcacac
     1621 aggccgaact tcatggccgt gggcggctgg gatggcatga cggcgatcta tgaagtggtg
     1681 cgccggctga acggcaaact cgacggcgac aaggcaatgg aagtgctcaa gggcctgaag
     1741 ctcgacagcc cgcgcgggcc ggtcaccatc gactcggaaa cccgcgacgc aatccagacc
     1801 atctatatcc ggcgggtgca gaaggtgaac ggcaagctct acaacgtcga gttcgagcag
     1861 gtgccgaatt tcaaggatcc gggaaaataa cctgtgctgg gtgctgggtg ctgggtgctg
     1921 ggtgctgggt gctgggtgca aaaagtctac tttccgccat ttccacctat tattttcctc
     1981 tgcgtccttt cttgcccttc ctctgcgccc tctgcgtaaa cgcttttcgt gccggcccgt
     2041 ggccggccgg gtggactgag caagggcgaa cccgtagaat gcgcggcttt ctccaatctc
     2101 caggcttggg cgcggatacc tcccggccgg ccagcggctc atgagtgaca tctccaggga
     2161 agttcttgcc gaaagagtcg agcagcggcg gaccttcgcc atcatttcgc acccggatgc
     2221 gggtaagacc acgcttacag aaaagctgct tcttttcgcc ggcgcgatcc acatcgccgg
     2281 cagcgtcaag gcgcgcaaag ccacccgcta cgccacctcg gactggatgg aaatcgaaaa
     2341 gcaacgtggc atttcggtcg ccagctcggt gatgcagatg gagtaccggg gcaaggtcgt
     2401 gaacctgctc gatacgcccg gccaccagga cttttccgaa gatacgtacc gggtgctgac
     2461 cgccgtggac gcggcgctca tggtgatcga cgccggcaac ggcgtggaag cacagacgct
     2521 gcggctgctg gaagtgtgcc gctcgcgcgg cacgcccatc ctcaccttca tcaacaagat
     2581 ggaccgggaa gtgcgcgaac cgctggacct gaccgatgaa atcgagcgtg tgctcggcat
     2641 ggccgcagta ccgttttcct ggccgatcgg catgggcaag acgtttcacg gcgtttacga
     2701 cctggcgggg gaccgcatcc gcgtgttccg cgccggcgat gaccggctgg ggcaggccga
     2761 cgaagtcatc agcggactgg accatccttc gctgcgtgaa cgcttcggca ccgagttcga
     2821 gcgagcgcgc cacgacatcg acctcattcg catggccacg cccgccttct cgcgcgagga
     2881 gtttctcgcc ggccggcaga caccggtgtt cttcggttcg gcgatcaaca acttcggcgt
     2941 tcgcgaagtg ctggatgcgc tggtggacat cgcgccgccc ccgcaaccga gagtggcgat
     3001 gcagggcaag gtcgatccgc atggcaagaa tttctccggt gtcgttttca agatccaggc
     3061 caacatggat cccgcgcatc gcgaccgcgt ggtgttcgtg cgcgtgtgct ccggacgttt
     3121 cgaacgcggc atgcgcgtca agattcagcg caccggcaag gagatccgcc caaacaacgt
     3181 ggtctccttc ctgtcgcagc gacgcgaact gctggacgaa gcctacgccg gcgacatcat
     3241 cggcattccg acccacggcg gcatccagct cggtgacaca ctgaccgacg gggcggaact
     3301 gcaatacgcc gggctgccat acttcgctcc ggaaatcttc cataccatcg aggtgttgaa
     3361 tcccttgcgt gccaagcagc ttcgcaccgg gctgatgcag cttggcgagg aaggtgcgat
     3421 ccaggtgttc cgcccattgc gcaactccat gatgctgctg ggcgcggtgg ggacccttca
     3481 gttcgatgtc gccgcgcacc gcctgcagca cgaatacgga gtggaagcgc ggttgatgcc
     3541 ggcttcatac acctgcgctc gctgggtgtc ggccgacgat cccgcggagc tcgagaggtt
     3601 cgtggaagcc aatgccggcc ggttggcgcg cgatgccgcc gatgcgctgg cggtactgac
     3661 caataccaag tacgaaatga aagtggtcca ggaccgctgg cccaacatca ggttccacgc
     3721 ccgacgcgaa cacgccgggt tagtccttga ccgaattgcc aatccggcct gacctccagc
     3781 gggaaatgcc agccctgcca cgcgcagatg ctgatcatgt cgtggaacgc cgcctcgccg
     3841 tattgatgaa ccacttcacc acatcgttcg gcgattgtta cgccgctctc gccggcatgc
     3901 atccaactca ctgacccgtg cgtaaatgca gttggacgcg ccgtcacggc ggcagaaaac
     3961 gcagccggga gtcagcgttc gtgccgggcg ccgggttacg gccggctcgc ggtcaccgcg
     4021 ggacgcggga cactcagccg ggcgcaaaca agaccggccg aagggcccga cccatctgaa
     4081 gaatctttca aagaaggagg ctgggaatgg acgttcatcg cagattggtc agcgcagcaa
     4141 tcgcaaccgc ggcagcggcg ctgtttgcaa ccggagtggt cggcaccgcg accgcccagg
     4201 aggtcaaggt caagtgttac ggggccaacg cctgcaaggg caagagcgag tgcaagactt
     4261 ccatgagcgg gtgcaagggc aaaaacggct gcaagggcca gggcttcgaa cagatgaccg
     4321 aaaaggcctg cgtcgagaaa ctgggccgca cttgatccat ggtctgtgaa tagagggcgc
     4381 cttcgggcgt cctctttttt cgtgcccccg gcagaaaacc tgaaatctca ccacgaaggc
     4441 gcgaggaaga ccttcgcatt gttcctgctt tgtgtccttg cctcattgcg gtgaaaggga
     4501 ttttcgttgg ggctatactg ccccattccg ttttgatcga ccgaaccggg ggatgcgatg
     4561 ggtgacatgc tgatggagca gcgcgcagaa ttcaaatcca tgaaggagag cacgaaggcg
     4621 gactgggaca tcatcgttgc gcgctaccag cccttcgcga aggaactccc gagccggatc
     4681 ctcgcgcact tgcggctgct cggcggcgac tccggcggct ttgcggtaga ccgcctgcaa
     4741 cactcgctgc tcaccgccac gctcgcgcac aaggccggag aggacgaaga gtacgtggta
     4801 tgcgcgctac tgcacgacgt cggcgatacg ctgggctcct ggaatcaccc ggaagtctcc
     4861 gccgccgtcc tcaagccttt cgtatcggag gccaaccact ggatggttga gaagcacggg
     4921 attttccagg gctacttctt cttccagcac gtcggcatgg atcggaacat gcgcgagcag
     4981 tttcgcggcc acccgcactt cgaacgcacg gcacgtttct gcgaaatcta cgacaaccct
     5041 gcattcgacc ccgggatgga atgtgcgccg ctggagtttt tcgaaccgat gctgcggaac
     5101 gtattttcgt tcccgaagaa ttcgatctac aagaatacgt gccagacgta gggcccggta
     5161 ccaggcctga tgatgggctg cctttatcct ccaactcgat ccttcgctga caatggccgg
     5221 tagatgcaag catctgtctg aagtccgcga cgtgacgccg agtgcgcagg gctgcgagga
     5281 ctgtctgaag accggcgaca cctggttgca cctgcgcatg tgcctgagct gcggccatgt
     5341 cggctgttgc gacagttcga agaacaagca cgccacgaaa cattcccaca ccacctccca
     5401 tccggtcatg aagtccttcc agccgggcga gcattgggga tggtgctacg tggacgagat
     5461 ttaccttcag ttttagaaga caggtcgccg ccgtcacgcg gacgggctga cgatgctggt
     5521 cgcgggaatg aaaacgggca ccgcaaggtg cccgtaccgt tatctgtcct gtgtcggcgg
     5581 ccggcagacc gcagattcaa gcgacggcgc gccgggaacg gccgcgccgt tcattaaagc
     5641 gccggtcggg gaatccgaca gcctgatacg ccaggcgacg gtcttgcccg cgcttatcac
     5701 cgaccgcccg tccttttctg atctgcgtcg gggaataagc ctgatacccg gtcaacaggg
     5761 cattgtgcaa gagttgaatg ctgcgctttg cggcaagcat gacccgcgtc tgcatcttct
     5821 ccgtcgtggc gtagcgcttg acgatctcca gtgcgatctt gggatgcaga
//
LOCUS       DASQOP010000178         4994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1686592, whole genome shotgun sequence.
ACCESSION   DASQOP010000178 DASQOP010000000
VERSION     DASQOP010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4994
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1686592"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /gene="ybgF"
                     /locus_tag="VE030_07280"
     CDS             <1..1007
                     /gene="ybgF"
                     /locus_tag="VE030_07280"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HZF23250.1"
                     /translation="RYDGLYDVQKDLNTLLVSLHEDHAKLTGEYKELHDNHQELQDRF
                     SLILDQVQTLSEEQKIAGQKLAQVESEGANRGVVQLLNRVEGLNTDMNRLRGQIEVLT
                     NDINNAQKRQRDMYVDLDTRLRRMEQQGAAARKDQEALPALEERIRKLEQAAAGLTIP
                     APVASAAAATSAASPGAVTAVSAPAVAPAATTVAAVPPSLPPANLSAVDQAAIQRAYD
                     NAYSNYRISDYQGAIRGFDGFLKTYPKHQLAPNAQYWIGESYFHLHDYRAAIEAQRRL
                     LGTYPDSAKVPDALLVIGTSETSLGDSAAARKTFEELIARYPSTESAEKAKGRLAKLK
                     "
     gene            1176..2288
                     /locus_tag="VE030_07285"
     CDS             1176..2288
                     /locus_tag="VE030_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HZF23251.1"
                     /translation="MGNFAHLANLVVALGFILGLVFGAVAHKANFCTMGAVADVVNVG
                     DWGRMRMWLLAIAVAVAGANALDWAGMVDLSRSAYPAARCPWIAYIVGGFLFGVGMTL
                     ASGCGSRSVVRVGGGNLKSLVVLVFLAISADMTMHGLFAPARVEVLSAVAIHFDQGQD
                     LPWVVATQFGVGRHGLQIWITALIVIPLLFFIFKDRTFRATREQVFGGFVIGLAVVAG
                     WYVTGHIGFVAEDPDTLQEAYLGSRTARPESLTYVGPVANTLEWLQLWADNSTKLSFG
                     VAVVAGTLLGSFAYAILTRNFRWESFASAGDTRNHIIGGILMGFGGVTAMGCTIGQGI
                     TGLSTLAVGSFLAFFSIIAGAAATMKVQYWRMLKEA"
     gene            2379..2454
                     /locus_tag="VE030_07290"
     tRNA            2379..2454
                     /locus_tag="VE030_07290"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2412..2414,aa:Lys,seq:ttt)
     gene            2843..4471
                     /locus_tag="VE030_07295"
     CDS             2843..4471
                     /locus_tag="VE030_07295"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZF23252.1"
                     /translation="MDRALKILLVEDVETDAELQVRELKRAGLNFDAHRVQSESAFLA
                     EISEFEPDIIMSDFSIPGFGGMRALELACNRVPETPFVFVSGTIGEETAVESLRRGAT
                     DYVLKTNLARLGPVVRRAIAEARERRGRFAAEQRAHALTNLYAALSESNDALARAQGR
                     DELFRDICRIAVARGGFKYAWVGLVDGEGGMVRPVVHFGAGSGAVERAEVPLDARQPG
                     GRGPMAMAAREGRSSVCNDILADRRFGAVHERMRVQEFRSTAALPLLLEGQPIGGFGL
                     YASEASYFDDERMHLLSDLAGNIAFALDRFEQEARRRRAETAILEARQQLQALSTRLI
                     EAQEDERREIARELHDEIGQALTLVKIKLQQAARLREGDTENLLEDCIEIADYTLKQV
                     RDMSLDLRPPLLDDLGLEAALEWTLQRREQAAGWQTAIATDPLPRRLAPEIETACFRI
                     AQEALTNVTRHAQAKGVDVSLRIVGNELVLAVRDDGRGFDPEAVRLRPAQRASLGLVS
                     MKERAALVGGRLEISTSDGGGTEVRATFPLRWRN"
     gene            4486..>4994
                     /locus_tag="VE030_07300"
     CDS             4486..>4994
                     /locus_tag="VE030_07300"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZF23253.1"
                     /translation="MKKTRVLLADDHTLMRAGMRVLLDDMAGYEVVAEAGDGDEALEL
                     LRQHRPDVVLMDIDMKRMNGLEAMATAKREFPNLHVIMLSMHAEKQYVMQALRDGASG
                     YLLKDAATVELELALNVVMRGDVYLSPGVSKQVAEGFVRGAQSVDPVASLTPRQREIL
                     TLIAEGHNT"
BASE COUNT          910 a         1526 c         1624 g          934 t
ORIGIN      
        1 tccgttacga cggcctgtac gacgttcaga aggatctcaa cacgcttttg gtgtcgttgc
       61 acgaagatca cgccaagctc accggcgagt acaaggagct tcacgacaac catcaggagc
      121 tgcaggatcg attttcgttg atcctggacc aggtgcagac tctcagcgag gaacagaaaa
      181 tcgccgggca gaaactcgcc caggtcgagt ccgagggcgc caatcgcggc gttgtgcagc
      241 tgctcaaccg cgtggagggt ctcaataccg atatgaaccg gttgcgcggc cagatcgaag
      301 tgctgaccaa cgacatcaac aacgcgcaga agcgccagcg cgacatgtac gtggatctgg
      361 acacgcggct gcgccgcatg gagcagcaag gcgccgccgc cagaaaggac caggaggcgc
      421 tgcccgccct cgaggaacgc atccgcaaac tcgaacaggc cgcagctggt ctgacgattc
      481 cggctccggt tgcgtccgcc gccgccgcaa catccgccgc ctcgccgggt gctgtgaccg
      541 cggtgtccgc acccgcggtt gcgcccgcgg ccacgaccgt ggcagcggtg ccgccttcgc
      601 tgcctccggc caacctttcc gcagtggatc aggcggcaat ccagcgcgcc tacgacaatg
      661 cctacagcaa ctatcgcatc agcgactatc agggtgcgat ccgcggtttc gacgggtttc
      721 taaagaccta cccgaagcac cagttggctc ccaacgcgca gtactggatc ggcgaatcct
      781 actttcatct gcacgactac cgggctgcaa tcgaagcgca gcgccggctt ctcggtactt
      841 atcccgacag cgccaaggta ccggatgcgc tgctggtcat cggcacctcc gaaaccagcc
      901 tgggagacag tgctgctgcc agaaagacct ttgaggaact cattgccagg taccccagta
      961 ccgagtctgc ggaaaaggcc aagggccgcc tcgccaagct caagtaggcg cctattacgt
     1021 acctcctccg gctctccccc tgaaggcgga gagcgaagaa gttcgttgcg ttgcattctg
     1081 gcggtcttga attcgtgccc accttcgcct ctacgcgccg gcgcgccgcg cagtgctaga
     1141 attttctccc gcacccggat gcgctgatcg actcgatggg aaactttgcc caccttgcaa
     1201 acctggtcgt cgcgcttgga tttatcctcg gattggtctt cggtgcggtc gcccacaagg
     1261 ccaatttctg caccatgggt gcggtggccg acgtggtcaa tgtcggcgac tggggccgca
     1321 tgcgcatgtg gctgctcgcc attgcggtcg cggtagcggg ggccaacgcg ctcgactggg
     1381 cgggtatggt ggatttgtcg cgctccgcct atccggcggc acgctgcccg tggatcgcat
     1441 atatcgtcgg cggattcctg ttcggcgtcg gcatgacgct ggcctccggc tgcggcagcc
     1501 gcagcgtggt ccgggttggg ggcggtaacc tcaagtcgct ggtggttctg gtgttcctcg
     1561 ccatttccgc ggacatgacc atgcatggcc tgttcgcgcc ggcgcgcgtg gaagtgttga
     1621 gcgcagtagc gattcatttc gaccagggcc aggatctgcc ttgggtcgtc gccacgcaat
     1681 tcggcgtggg ccggcacggc ttgcagatat ggataaccgc gttgattgtg atcccgctgc
     1741 ttttctttat atttaaggac aggacgttcc gcgccacgcg cgaacaggtg ttcggggggt
     1801 ttgtgatcgg gctggcagtc gtcgccggct ggtacgtcac cgggcatatt ggcttcgtcg
     1861 ccgaagatcc ggatacgctg caggaagcct acctgggttc caggaccgcc cgcccggagt
     1921 cgctgaccta tgtcggcccg gtcgccaaca cgctcgagtg gctgcagctc tgggccgaca
     1981 actccacgaa actgagcttc ggcgtcgccg tcgtagccgg cacgctgctc gggtcgttcg
     2041 cctacgcaat tctcacccga aacttccgct gggaaagctt cgcatccgcg ggtgataccc
     2101 gcaatcacat catcggcggc attctgatgg gcttcggcgg cgtcaccgcg atgggctgta
     2161 ccatcggcca gggcataacc ggtctttcca cgctcgcggt cggctctttt ctcgcgttct
     2221 tctcgatcat cgccggcgcc gccgcgacca tgaaggttca atactggcgc atgctcaagg
     2281 aagcctgaac gcgcgcagaa caagaatcca cgcggttttc cggacattcg cgtttgacgc
     2341 accaacggcg ctgccggtac aatgcgcgct ctttttttgg gcggttagct cagtcggtag
     2401 agcagcggac ttttaatccg ttggtcgcga gttcgaatct cgcaccgcct accagcatga
     2461 aaagccggcg ctgacctggc tttttttggc ccattcgcga tcggacaatt tgcgattcgg
     2521 gaactgggcc acgccggctc tgaagtttcc cggagcggcg ctcggcactt gcgtgacgat
     2581 gcgtcggctc ccgtcaaccg atttcctcgt tgcttcggcg tcattaaaat ggttttgcgg
     2641 ctgcgcgtgt taccctcacg ggcaggggca cgtcgggaca gcatgcgctt gtgcgagttt
     2701 tgcctacaca gcggcggtgc tgatgcggga gaatcagtat tgcattccgg caaatgcccg
     2761 gaagtggtgg cggcgcgcgt cttgtgccgg caccggaagg cgatgcaatg aactggaatc
     2821 gttgggcata gcaaccctaa ccatggaccg tgcgctcaaa attctgctgg tcgaggatgt
     2881 cgaaaccgat gccgaactgc aagtgcgcga actcaagcgc gccggcctga acttcgatgc
     2941 gcaccgggtg cagtccgaat cagcgtttct cgcagaaatc tccgagttcg agcccgacat
     3001 catcatgtcg gatttctcca ttcccggctt cggcggaatg cgggcgctcg agcttgcctg
     3061 taaccgcgtt ccggaaaccc cgtttgtctt cgtttccgga accatcggcg aggaaacagc
     3121 cgtcgagtcg ctcaggcgcg gcgccaccga ctatgtgctg aagaccaacc tggcgcgtct
     3181 cggcccggta gtgcggcgcg cgatcgcaga ggcgcgggag cggcgtggac gtttcgcggc
     3241 cgaacagcgc gcccacgcgc tgaccaatct gtacgcggca ctttccgaaa gcaatgatgc
     3301 gttggcacgg gcgcaggggc gcgacgagct gttccgcgac atctgccgca tcgcggtggc
     3361 gcgcggcggc ttcaagtatg cttgggtggg attggtggac ggcgaaggcg gaatggtcag
     3421 accggtggtg catttcggtg ccggcagcgg cgcagtcgag cgcgccgagg taccgctcga
     3481 cgcgcgccag cccggcggcc gcgggccgat ggcgatggct gcgcgcgagg gccggtccag
     3541 cgtttgcaat gacatcctgg ccgatcgtag attcggcgcc gtgcacgagc gcatgcgcgt
     3601 gcaggaattt cgctccacgg ctgcactgcc gctgctgctt gaaggccagc ccatcggcgg
     3661 atttggcctg tatgccagcg aggcgagtta ttttgacgac gagcgcatgc atctgctgag
     3721 cgatctggcc ggcaacattg cctttgcact tgaccgcttc gaacaggaag cgcggcgcag
     3781 gcgcgcagaa acggcaatac tcgaagcaag gcagcagttg caggcgctgt ctacgcggct
     3841 catcgaagcg caggaggacg agcggcgcga gatcgcgcgg gagttgcacg atgagatcgg
     3901 gcaggcgttg acgctggtta aaatcaagtt gcaacaggca gcgcggctgc gcgagggcga
     3961 tacggagaac ctactggagg attgcatcga gatcgccgat tacacgctca agcaggtgcg
     4021 tgacatgtcg ctggacctgc gcccgccgct gctcgacgac ctcggcctcg aggcggcgct
     4081 ggagtggacc ctgcagcgcc gggagcaggc tgccggatgg cagaccgcaa ttgccacgga
     4141 tccgctgccc agacgtctgg caccggagat cgagaccgcc tgtttccgca tagcccagga
     4201 ggcgctaacc aacgtgacgc gccacgccca ggcgaaaggg gtggacgtga gcctgcgcat
     4261 cgtcggcaat gagttggtgc tcgcggtgcg tgacgatggc aggggattcg atccggaggc
     4321 ggtgaggctg cgcccggcgc agcgcgccag cctcggcctg gtgtcgatga aggagcgcgc
     4381 cgcgctggtc ggcggccggc tcgaaatcag cacctcggac ggcggcggca ccgaagtccg
     4441 ggccacattt ccgctgcgct ggcgtaactg acgcatggac gatcgatgaa gaaaacccgc
     4501 gtgttgctgg cggatgacca cacgctgatg cgtgcgggca tgcgggtatt gctcgacgat
     4561 atggccggct atgaagtcgt cgccgaggcc ggcgacggcg atgaggcact cgaactcctc
     4621 cgtcagcatc ggccggacgt agtgctcatg gacatcgaca tgaagcgcat gaatggcctg
     4681 gaggccatgg cgacggcaaa acgggagttc ccaaatctgc atgtaatcat gctctcgatg
     4741 cacgcagaaa agcagtacgt gatgcaagcg ttgcgcgacg gcgcatccgg atacttgctc
     4801 aaggatgcgg caacggtaga gctcgaactg gcgctcaacg tggtgatgag gggcgacgtg
     4861 tacctgagcc ctggggtgtc gaagcaggtg gccgagggtt tcgtgcgtgg ggcccaatcc
     4921 gtggatccgg ttgcttcact gacgccgcgc cagcgcgaaa tcctcacgct catcgccgaa
     4981 ggccacaaca ccaa
//
LOCUS       DASQOP010000179        18020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1284481, whole genome shotgun sequence.
ACCESSION   DASQOP010000179 DASQOP010000000
VERSION     DASQOP010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 18020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18020
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284481"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..2025
                     /gene="pgaB"
                     /locus_tag="VE030_07305"
     CDS             55..2025
                     /gene="pgaB"
                     /locus_tag="VE030_07305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HZF23254.1"
                     /translation="MSLRAFPFALLALRLAAGAMGMTVIGTAAQAAESAVSYRVLCYH
                     DVQEDVRLNPDPYAVGSAQLVAQFAWMKDNGYRVVSLDDVIAAREGRRALPAKAVVLT
                     FDDGYRSVYTRVFPLLKLFNYPAIVALSGRWLDAAPGETVEYEGKQIARERFLSWEQI
                     REMADSGLVEMASHSYDLHRGIPANPQGSLIPALIAHRYDNGRYEDEPAHIARLRADF
                     ARNSTLIESKAGRRPRVMVWPFGRDSIQAIAVARELGMPVTMSLVSGANNLKDDLSRI
                     RRDELVHNPPLRDFISVVSKDPDPLPERVVQVDLDYVYDADPRQQQANIDGLLDRILA
                     LRVTTVYLQAFADADGNGQAEAMYFPNRHLPLRADLFSYVAWQLSTRAFVKVYAWMPV
                     LAFELPSSNSAAALTVQSADADAASAHPRYRRLSPFSADAQRVIGEIYEDLARHASFD
                     GLLFHDDAVLDDFEDASPAALQTYREWGLPASIEAIRSDPQLLRRWTELKTRALIDFT
                     GELTERVRDYQGAIKTARNLYARPVLEPAAQAWFAQSLPDFLAAYDYTAVMAMPLMEG
                     AAKPDAWLDALVRKVSAEPGALKKTVFELQSMDWNTRERVPTRTLAGQMNRLQRLGAI
                     NFGYYPDDFHADHPGLAGIKPAISLQSFLRND"
     gene            2044..3309
                     /gene="pgaC"
                     /locus_tag="VE030_07310"
     CDS             2044..3309
                     /gene="pgaC"
                     /locus_tag="VE030_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HZF23255.1"
                     /translation="MTDTFGTIGAILFGFAFYYPLFMAYNWMIGALYYFFHYEAHQGK
                     VDQPPGLERYPGVTFLVPCFNDEKIVAETIEYLLQQKYPDFEVIAINDGSRDRTGDIL
                     DALAVKHPRLRVIHHAANQGKAMALTSGALLTPNEYLICIDSDALLDQHAARWMMWHF
                     MSSTRVGAVTGNPRIRNRSTLFGRIQVGEFSSTAGLLKRAQRTYGRLFTISGVIAGFR
                     KMALHQAGYWSQDMLTEDIDVTWKLEIAKWDVRFEPRALCWILMPETLSGLWRQRLRW
                     AMGGAQVIFRHFRKLHDWRERRMWPIYLEYFTSMLWAYTMATTVILWGFGKLIEVPQA
                     LQVPTLLPGFSGVLIGITCMLQVAVGLLMDSRYEKGLFRYYFWMIWYPVAFWIINVLT
                     TVVATPRVLMRRNGKRATWRSPDRGIHTT"
     gene            3306..3776
                     /gene="pgaD"
                     /locus_tag="VE030_07315"
     CDS             3306..3776
                     /gene="pgaD"
                     /locus_tag="VE030_07315"
                     /inference="COORDINATES: protein motif:HMM:TIGR03940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HZF23256.1"
                     /translation="MNDIVFDLRNIIIDRPELQSKGKRAIFGVLTAGLWSFYLYLLLP
                     LASLLAWWLGFTAVYEELVMRRGWEALLELIGVYGAIVLAMGLVQVSWALGNWLRFYG
                     ARDRRRERPQEVNQEFTDPLFLMDTGEFPVWMNAKRLVVHHHPKYPRITSVEAG"
     gene            4009..5427
                     /locus_tag="VE030_07320"
     CDS             4009..5427
                     /locus_tag="VE030_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HZF23257.1"
                     /translation="MQLDQESCYQALVSRDPRFDGLMFVGVSSTGIYCRLICNSRRPL
                     RRNCSFYPNAAAAEQAGFRPCLRCRPELAPGNALVDSRNRLAVALARRIEDGALNEGS
                     VSALAAEMGISDRHLRRVIQDEFGVSPNKLLETQRLLTAKLLLTDTTLPVIDVAFASG
                     FSSLRRFNDAFKSHYRLSPTGFRRSTACAGSGRFVCELAYRPPLDWDAMLEFLSKRLY
                     DGVEAIRDSSYLRTVSLGGRTGWIRVRPSAQRHALTVELAPELGRAIPAVLSRVRRLF
                     DLGAEPQRIAQRLGKLAARQPGLRLPGAFDGFEVAVRAVLGQQISVKAASTLAGRFAA
                     AFGEPVTMPFPELGRLMPTAQRVASAAPPALTKIGVTSARARTLGNVARAMVANPGLL
                     DPGADVEAALSRIKAIPGIGEWTAQYLGMRAFSWPDAFPATDLGIRKALGTDDEREIL
                     ARAEAWRPWRGYAAMHLWRTLT"
     gene            5424..5978
                     /locus_tag="VE030_07325"
     CDS             5424..5978
                     /locus_tag="VE030_07325"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HZF23258.1"
                     /translation="MTTFYTTFESPVGPLLLMSDGQSLTGLHTDSDKQRPVMRPDWIR
                     DEAAVPFAEVIAQLRGYFEGRVTEFDLPLAPNGSEFQLRVWRELRGIPYGETISYAEL
                     ARRIGRPQASRAVGHANARNPVSIIVPCHRVIGADNSLTGYAGGLERKRMLLAHEAKH
                     RGMRDEGREARGKSNKGQLSLAGM"
     gene            6149..7603
                     /locus_tag="VE030_07330"
     CDS             6149..7603
                     /locus_tag="VE030_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HZF23259.1"
                     /translation="MPRTAHRVERDLLGERNVPATAYYGIHTLRALENFPISGTSIAL
                     HGDLITALACVKQACAISNHKLGFLDRSRARAIIAACEEIRAGKLRAEFVVDVIQGGA
                     GTSTNMNANEVIANRALEHLGRRRGEYEYLHPLEHVNMSQSTNDVYPTAVCLGLHFAI
                     DGLLEAMALLQRAFNGKARAFVKVIKLGRTQLQDAVPMTLGQEFSTYAVMLGEDRMRL
                     REASRLIEEINLGATAIGTGINTVPGYARLVRRELTRISGVKLITAPNLVEATQDAGA
                     FVQLSGVLKRIAVKLSKVCNDLRLLSSGPMAGFNEINLPPMQAGSSIMPGKVNPVIPE
                     VVNQVAFEVIGHDVTVSFAAEGGQLQLNAFEPIIAHSLFTSVSHLRRGCVVLAERCVK
                     GITANQKHLARMVKDSPSIATALNPYIGYEKATYIAKKATTTGQSVYDLVVNEGLLSR
                     KEVDKILRPEVLTQPQPLRAPSMRRRSTKRPNAR"
     gene            complement(7639..9144)
                     /locus_tag="VE030_07335"
     CDS             complement(7639..9144)
                     /locus_tag="VE030_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZF23260.1"
                     /translation="MNLHHEVQILLNALGASAGDSTEGLCARSPIDGSRIGGVVLDDE
                     ASIREKVARSREAFRHWRMTPAPKRGELIRLFGEAVRNNKAALGRLVSIESGKILQEG
                     LGEVQEVIDICEFAVGLSRQLHGLTIASERPGHRMMETWHPLGPGVVITAFNFPVAVW
                     AWNAALALVCGNTMLWKPSEKTPLTAVAMQALLERTCREIGNAPHQLNEVVFGASDQG
                     RILLDQPEIRLASATGSTRMGRAVAAQLAPRFARTILELGGNNAAIVAPSADLALTER
                     AILFSAVGTAGQRCTSLRRLFVHRSLFDGFLGRLKSLYARAKVGNPLDSDTLVGPLID
                     SNAYAGMRAALDRANVDARWIAGGERVDTEYPGGYYVRPALAAMRSQTDIVLEETFAP
                     ILYAMPYDTLDEAIQLHNAARHGLSSSIFTQDLREAELFMSSAGSDCGIANVNIGTSG
                     AEIGGAFGGEKDTGGGRESGSDAWKAYMRRATNTINYSGELPLAQGIRFEL"
     gene            complement(9152..10243)
                     /locus_tag="VE030_07340"
     CDS             complement(9152..10243)
                     /locus_tag="VE030_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HZF23261.1"
                     /translation="MARRVLLLGTGHIGRAVAHLLVTAEGEPRYEVAVGDRLVDPELR
                     SLFSDRWFEIDGDDIGSFARRIQGHDIVINALPFHLADTVANAAARAKAHYLDLTEDV
                     STTRAIRALAEGAPIAFMPQCGLAPGFIGIAGYALSQGFDKLERLHMRVGALPQYPTN
                     ALKYNLTWSVDGLINEYVHPCEAIVDGRRIELPALEGYETFALDGVNYEAFNTSGGLG
                     TLAETLEGHVQYLDYKTIRYPGHRDIAKLLLQDFGLAGKRETMKDILQTAIPATEQDV
                     VVVFVTASGLRGGKRTQVSFARKVYAQTWSGMPMTAIQITTASGVCAVMDLFVQGKLP
                     QSGFLRQEQVALTDFLSNRFGRAYEAAAT"
     gene            10553..11449
                     /locus_tag="VE030_07345"
     CDS             10553..11449
                     /locus_tag="VE030_07345"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZF23262.1"
                     /translation="MEQEMFGFVGVGKMGSLMSSRLLNAGQRVCVFDTDEKAVAALEK
                     AGAQRAGSAAEVAGIAQTVFLSLPNPEIVQNVALGRGGVIEGTRVKHVADFSTIGPRT
                     AGIVARALAERKITYVDAPVSGGLRGAREGTLAVMVSCPRNVYAVLEPVLKTFGKPFH
                     LGEVAGQAQTMKLANNLLAAAAIAVSSEAMVMGVKAGLDPQMMLDILNAGTGRNSATQ
                     DKFPRAILPGTFDFGFTTGLSYKDVRLCIDEAESLGVPMVVGAAVRQMLAVTNAVYGA
                     DSDFTSMCRVVESWAGVEVRSK"
     gene            11463..12854
                     /locus_tag="VE030_07350"
     CDS             11463..12854
                     /locus_tag="VE030_07350"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HZF23263.1"
                     /translation="MVTVHPVKRGPAAQTGEPLAARIAHSVVSMSLNDLPAEATDKVK
                     LCLFDLIGCAFESRDLPWSRQAAALAEGTGGAATVIGSPHPASHADAAFANAVMGHGL
                     VREDMHSGSISHLGIVVLPALLALSELRHVRGRNFITAAVVGYEVGGQIGRAVMDAEV
                     ARIHRPTGITGPVAAAAAGARLLGLSTDAVISAIALGANTTVGFNQWAHTGGSEMFFH
                     AGYAARDAVTAARLAEMGAFASPSALDGEAGLFAALGKRPAAAKIALFQGQPEILSVY
                     HKPVPACNFAQTASQAALAIAHEHKPKPERIAAITVRVPRAGALYPGCDYTGPFAHVL
                     QAKMSIQYNVAAALIEGGVSEQNFTRLQDPGLQRLIGVMKLEIDEAMTRAYPGKQGGE
                     VEVRESGGATHRIRLEDVVNASAADVRARFRAAAENVLGAARASEIESFIDGIELRKD
                     VAQLGALLRAPAT"
     gene            13008..13964
                     /locus_tag="VE030_07355"
     CDS             13008..13964
                     /locus_tag="VE030_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZF23264.1"
                     /translation="MTAAQLLENFLQALTAGLLTGAIYGLMCVGLSVIFGVMRVINFA
                     QGDFMMLGMYVAYYAFGLFGAHAVFGTAMDPYIAALLAGPVIFVAGVIVHKLLVSRVT
                     GIRGSALESEGHYAQLILTLGVALILQNGGLYVFGSTPVSIATPLSSSAWALGPLYRD
                     ATVFVNQGQAVAAVIAVVAVLGFVLFMARSRLGKSLRAAADNAEAAIYMGIDVDRAHC
                     LAFGFGVGMTAIGGGLLASGGPFQPYVGLEYVIVMYAGVVLGGLGSVSGAFFGGLTIG
                     LVQQLSTLFLPNQLQNTAIFVVFLVIVLLRPQGLFGRAARRT"
     gene            13990..15756
                     /locus_tag="VE030_07360"
     CDS             13990..15756
                     /locus_tag="VE030_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HZF23265.1"
                     /translation="MNLRRQALLIAAFGIVYAVATAFAENSYYQLIMTVVPVWAVMGL
                     AWNVLSGYSGLVSFGHAAFFGLGAYSLALAQIHWGLSPWLGIPLAGMVGALAGLVVGF
                     PTFRLRGHYFALAMLAYPLALLYVFEWLGYQELTLPMQRQAPLAYMQFEDHRIYAWLA
                     LALMLGAMVLTGLIERSRFGMSLLAIKQDEAAAEAAGIRTLAWKLRAITLSGAMAGTI
                     GGFYAVVLLVVTPPAVFGMLVSAQALIVAMFGGVGTLWGPVIGAVILVPLSELLHAKL
                     GNVIPGIQGVVYGIAIVAVILLAPEGVFWKVKDLMLRRRVGKNLDQPQSAGPASAVVA
                     ELPLCKKGGDGAVVLEVRNVSKAFGGLQAVRDVSMKVVSGEILGIIGPNGAGKTTFFN
                     LLNGFIRPDQGQILLEGRDIVGARPYEICAAGVGRTFQVVRPFRRMTVLENVVVGAYV
                     HAASDREARANAEAALARVGLDRDAQLLAGSLSNKQLRLLELARALAGRPKVLLLDET
                     LAGLGGGEVEEVIALVRKLAARGVTIVIIEHTMQAMVRLVDRFVVLNDGAVLAEGQPA
                     DITRNPAVIDAYLGKRWRVANA"
     gene            15749..16456
                     /locus_tag="VE030_07365"
     CDS             15749..16456
                     /locus_tag="VE030_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZF23266.1"
                     /translation="MLEIEALGAGYGAVPVLNDVNIRVGAGEFVAIVGPNGAGKTTLF
                     KTISGIVPPMSGVIRFEGRDLLAVAPSARPHLGIAHVPEGRQVFPSLTVLENLEMGAA
                     TETGRRNWAENLEKVYTWFPVLAERAKTQAGALSGGQQQMLAIARGLASSPKLLMLDE
                     PSLGLAPAVADEIFERIVAIHAGSGLTVLMVEQRVAEALHFATRGYVLEAGRVVLEGS
                     HDELKSNDRIRQAYLGM"
     gene            16511..17749
                     /locus_tag="VE030_07370"
     CDS             16511..17749
                     /locus_tag="VE030_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZF23267.1"
                     /translation="MKINALGRHAVRALLAAAMVAAAITATGQTKEVEVGLIAPMSGP
                     YARQGQVMKMAADMAIETVNQQGGIKSMGGAKLKLSVFDAGDSVERAKNAAQRMVANA
                     PDLVVATGAYLSSFTLAVTEVTERAELPVITFSYSDLITSRGFKYVFQTSATGVQQAE
                     GSLPALMEMAEKASGKRPQTIGIVMDNTAASVSFVKPMRDHQFAKLGLKLVVDETFTP
                     PLANATPLIQKVRSARPDILILLPTVISDAKLLLEKMNEFGLGRGKLPTIANGAAILD
                     PDLRNNMNIEQLEGVMAVVANWTTSAQKDFVAEVKAKTNEPWPTQHFVTTYGDILIFK
                     AALEAAGKADRQAVAQALRTMNLSGGAANFFPGNGKLRFEENGRRADASLLIVQWQKG
                     VPITVYPPKDALAGPAWPTQ"
BASE COUNT         3253 a         5631 c         5857 g         3279 t
ORIGIN      
        1 gcgtgcgcga gtacgccaac ttttacaacc tgtcgctgaa ttggagattt tgagttgagc
       61 ctgcgcgcct tcccgttcgc tctcctcgcg ctccggctcg ccgccggtgc gatgggcatg
      121 acggtgatcg gcacggccgc gcaggccgcc gagagcgcgg tgtcgtatcg cgtgctgtgc
      181 tatcacgacg tgcaggaaga cgtgcggctg aacccagacc cctacgcggt gggcagcgcg
      241 caactggtgg cgcagttcgc ctggatgaag gacaacggct accgcgtggt gagcctcgac
      301 gatgtgattg ccgccagaga agggcgccgg gcgctgccgg cgaaagcggt ggtgctgact
      361 ttcgacgacg gttatcgcag cgtctacacg cgcgtgtttc cgctgctgaa gctgttcaac
      421 tatccggcca tcgtcgcgct ctccggccgg tggctcgatg cggcgccggg cgagaccgtc
      481 gagtacgaag gcaaacagat tgcgcgcgaa cgcttcctgt cgtgggagca gatcagggaa
      541 atggccgatt cggggctggt cgaaatggcc tcgcacagct acgacctgca ccgcggcatt
      601 cccgccaatc cgcagggcag cctgattccg gcgctgatcg cgcaccgcta cgacaatggc
      661 aggtatgagg acgagcccgc ccacatcgcg cgactgcgcg ccgacttcgc ccgcaactcc
      721 acgctgatcg agtcaaaggc cgggcgccgg ccccgggtga tggtgtggcc attcggccgc
      781 gacagcattc aggcgatcgc agtcgcgcgc gaactcggca tgccggtcac catgagtctg
      841 gtgagcggcg ccaacaacct caaggacgat ctgagccgga ttcgacgcga tgagctggtc
      901 cacaacccac cgctgcgcga cttcatctcg gtggtctcca aggaccccga cccgctgccg
      961 gaacgggtgg ttcaggtgga cctcgactat gtttacgacg ccgacccgcg ccagcaacag
     1021 gcgaacatcg acggcctgct cgatcgcatc ctcgcgctgc gcgtgaccac cgtgtatctg
     1081 caggcattcg ccgacgccga cggcaacggc caggcggagg cgatgtattt cccgaaccgg
     1141 catctgccgc tgcgcgccga cttgttctcc tacgtcgcct ggcagctttc gacgcgcgcc
     1201 ttcgtcaagg tctacgcctg gatgccggtg ctcgcgttcg agctgccgtc cagcaattcc
     1261 gcggccgcgc tcacggtgca gtcggccgat gccgatgccg cgtcggcgca cccgcgctac
     1321 cggcgcctgt ccccgttcag cgccgatgcg cagcgcgtga tcggggaaat ctacgaagac
     1381 ctcgcccgcc acgcctcctt cgacggcctg ctgttccacg acgatgccgt gttagacgat
     1441 ttcgaggacg caagcccggc ggcgctgcag acctaccggg agtggggact gccggcatcg
     1501 atcgaggcga tccgttccga tccgcaactg ctgcggcgct ggaccgaact gaagacccgc
     1561 gcgctgatcg atttcaccgg cgagctcacg gagcgcgtgc gcgattacca gggcgccatc
     1621 aagaccgcgc gcaatctcta cgcccgtccg gtgctggagc ccgcagcgca ggcctggttc
     1681 gcgcaatcgc tgcccgattt tctcgccgcc tacgattaca ccgcagtgat ggcgatgccg
     1741 ctgatggaag gcgcggcgaa accggacgcg tggctcgacg cgctggtacg caaagtgtcc
     1801 gccgaaccag gagcgctgaa gaagaccgtg ttcgaactgc aaagcatgga ctggaacacg
     1861 cgcgagcgcg tgcccacgcg gacgctggcc ggacagatga accgcctgca gcgcctgggc
     1921 gcgatcaact tcggctatta cccggacgat ttccacgccg atcatcccgg gctggcgggc
     1981 atcaagccgg cgatttcgct gcagagtttt ctacggaacg actgatggcc aggagtgcag
     2041 atcatgactg ataccttcgg caccatcgga gccatcctgt tcgggttcgc gttctactac
     2101 ccgctgttca tggcctacaa ctggatgatc ggcgcgctct actacttctt ccactacgaa
     2161 gcgcaccagg gcaaggtgga ccagccgcca gggctggaaa ggtatcctgg cgtgactttc
     2221 ctggtgccat gcttcaacga cgaaaagatc gttgccgaaa ccatcgagta cctgttgcag
     2281 cagaagtatc cggacttcga ggtgatcgcc atcaacgacg gcagccgcga ccggaccggg
     2341 gacatcctcg atgcgctcgc ggtgaagcat ccgcggctgc gcgtcatcca ccacgccgcc
     2401 aaccagggca aggccatggc gcttaccagc ggcgcgctgc tgaccccgaa cgaatacctg
     2461 atctgcatcg attccgacgc gctgctcgac cagcacgcgg cgcgctggat gatgtggcat
     2521 ttcatgtcga gcacgcgcgt cggcgcggtc accggaaacc cgcgcatccg caaccgttcc
     2581 accctgttcg gccgcatcca ggtcggtgaa ttttcttcca ccgccggcct gctcaagcgc
     2641 gcgcaacgca cttacggtcg gctgttcacc atatcgggcg tgatcgcagg cttccgcaag
     2701 atggcgctgc accaggccgg ctactggagc caggacatgc tcaccgagga catcgacgtt
     2761 acctggaagc tcgagatcgc caagtgggac gtgcgcttcg agccgcgcgc gctgtgctgg
     2821 atcctgatgc cggaaacgct ctccggcctg tggcgtcagc gcctgcgctg ggccatgggc
     2881 ggcgcgcagg tcatcttccg ccatttccgc aaattgcatg actggcgcga gcggcgcatg
     2941 tggccgatct acctggaata cttcaccagc atgctgtggg cctacacgat ggcgaccacc
     3001 gtgatcttgt ggggattcgg aaagctcatc gaggtgccgc aggcgctgca ggtgccgacg
     3061 ctgctgcccg gcttcagcgg cgtgctgatc ggtatcacct gcatgctgca ggtcgccgtc
     3121 ggcctgctga tggattcgcg ctacgaaaaa ggtttgttcc gttactactt ctggatgatc
     3181 tggtacccgg tcgcgttctg gatcatcaac gttctcacca ccgtcgtcgc cacgccccgg
     3241 gtcctcatgc gccggaacgg gaagcgtgcc acctggcgca gtcccgaccg aggtatccac
     3301 acgacatgaa cgacatcgtc ttcgaccttc gcaacatcat catcgaccgg cccgagctcc
     3361 agtcgaaggg caagcgcgcg attttcggcg tgctcaccgc gggactctgg agcttctacc
     3421 tttatctgtt gttgccgctg gcgagcttgc ttgcatggtg gctcggattc acggcggtgt
     3481 acgaagagtt ggtgatgcgt cgcggctggg aagcactgct cgagctgatc ggcgtctacg
     3541 gcgccatcgt cctggcgatg ggtctggtgc aggtgagctg ggcgctgggc aactggctgc
     3601 gtttctacgg cgcgcgcgac cgccgtcgag agcgcccgca ggaggtgaac caggaattta
     3661 ccgacccgct gttcctgatg gataccggcg aattccccgt atggatgaac gccaagcgtc
     3721 tggtcgttca tcaccacccc aagtacccgc ggatcacgtc agtcgaagcc ggctgacccc
     3781 gcctgcccaa atcttcacca cagagacaca gagacgcaga ggaaaaccga caacaggcgt
     3841 aaatgggcaa caagcagaat tccggtaatg tacgttcaat acttgggatt ccctctacta
     3901 tatcttgtag ccctccgtgc ctctgtgtct ctgtggtaaa agttccgtcc tgaactgcat
     3961 gtccgaaaat ggctagcgcc tgcccgagag agagggcaac atgggcgcat gcaactcgat
     4021 caggaaagct gttaccaggc gctggtgagc cgggatcccc gcttcgacgg gctcatgttc
     4081 gtcggcgtct cctccaccgg aatctactgc cgcctcatct gtaattcgcg gcggcctttg
     4141 cgccgcaact gcagcttcta tccgaacgcc gccgcggcgg agcaggcggg cttccgtccc
     4201 tgcctgcgct gccgtcccga gctcgcgccc ggtaacgcgc tggtcgattc ccgcaaccgt
     4261 ctcgcggtag cgctggcccg gcgcatcgag gacggcgccc tcaacgaggg cagcgtgagc
     4321 gcgctggcgg cggagatggg catcagcgac cgccacctgc ggcgcgtgat ccaggacgag
     4381 ttcggcgtgt cgccgaacaa gctgctcgaa acccagcgcc tgctcaccgc caagctgttg
     4441 ctcaccgaca ccacattgcc ggttatcgac gtggctttcg ccagcggctt ctccagcctg
     4501 cggcgattca acgacgcctt caagagccat tatcgcctca gcccgaccgg cttccgccgc
     4561 agcaccgctt gcgccggtag cggccgcttc gtctgcgaac tggcatatcg gccgccgctc
     4621 gactgggacg cgatgctcga gttcctgtcg aagcggctgt acgacggcgt cgaggcgatc
     4681 cgcgattcga gctatttgcg cacggtgagc cttggcgggc gtaccggctg gatccgcgtg
     4741 cggccgagcg ctcagcgtca tgcgctgacg gtagagctgg cgccggaact tgggcgcgcg
     4801 attcccgcgg tgctgagcag ggtgcggcgg ctgttcgacc tgggcgcgga accgcagcgt
     4861 atcgcgcagc gcctgggcaa gctcgctgcg cggcagccgg gcctgaggct gcccggtgcg
     4921 ttcgacggtt tcgaggtggc ggtgcgtgcg gtactcgggc agcagatcag cgtaaaggcg
     4981 gccagcacgc ttgccggtcg cttcgccgca gcattcggcg agccggttac gatgccgttt
     5041 ccggaattgg ggcggctcat gcccaccgcg cagcgcgtcg cctccgccgc gcccccagcg
     5101 ttaacaaaaa tcggcgtgac ctcggcgcgg gcgcgtacgc ttggcaacgt cgccagggcg
     5161 atggtggcaa atccgggcct gctcgatcca ggcgccgacg tggaggccgc gctgtcgcgc
     5221 atcaaggcga tcccgggtat cggcgagtgg actgcgcaat accttggaat gcgcgctttt
     5281 tcctggccgg acgcgtttcc ggccaccgat ctcggcatcc gcaaggcgct gggcaccgac
     5341 gacgagcgcg agattctggc gcgcgcggag gcctggcggc cctggcgcgg ctacgctgca
     5401 atgcatctct ggagaacact gacatgacaa ctttttatac gacattcgaa agccccgtcg
     5461 gtccgctgct gctgatgtcc gatggacaat cgctcaccgg cctgcacacc gacagcgaca
     5521 agcagcgtcc cgtgatgcgg cccgactgga ttcgcgacga agccgccgtg ccgttcgcgg
     5581 aagtgatagc acaattacgt ggctatttcg aaggccgggt gaccgaattc gatctgccgc
     5641 tggcaccgaa tggcagcgaa ttccagttgc gcgtgtggcg ggaactgcgc ggcatcccct
     5701 acggcgagac catctcttac gcggaactgg cgcgccgcat aggacggccg caggcctcac
     5761 gcgcggtcgg ccacgccaat gcgcgcaacc cggtttccat catcgtcccc tgccatcgcg
     5821 tcatcggagc cgacaactcg ttgaccggtt acgcgggcgg cctggagcgc aagcgcatgc
     5881 tgctcgcgca tgaagcgaag catcgaggga tgagggacga gggccgagag gcgaggggaa
     5941 agtcgaataa agggcaactc tccctggctg gcatgtaagg cgcgagattc cgcgccgctg
     6001 ttcgccggag attcattctc acggcgcgcg catccgttgg aacgcggctc aaggaggtgg
     6061 aaatcaccaa tggcgtgagc gcggacctgg cgctccgcgc gctgactccc aatgtatgct
     6121 atcgctttga gcacaaggga gtaacgcgat gccaagaacc gcccacaggg ttgagcgcga
     6181 tttgctcggg gagcgcaatg ttcccgcaac tgcctactat ggcatccaca cgctccgggc
     6241 cctggagaat tttcccatca gcggtacttc catcgctctg catggggatc tgatcacagc
     6301 cctggcctgt gtgaagcagg cttgcgcaat atccaatcac aaacttggtt ttctcgatcg
     6361 cagtcgtgcc cgggcaatta ttgcggcgtg tgaagagatt cgtgccggca agctgcgtgc
     6421 ggagttcgtg gtggatgtca tccaaggcgg tgcgggaacg tccaccaaca tgaacgccaa
     6481 cgaggtgatc gccaaccgtg cgctggagca cctggggcgt cgccgtggcg agtatgaata
     6541 tctgcatcct ctggaacatg taaacatgag ccagagcacc aacgacgtgt atcccactgc
     6601 ggtgtgcctg ggccttcatt tcgccatcga tggtctgttg gaggccatgg cgttgcttca
     6661 acgagccttc aacgggaagg cgagggcctt cgtaaaggtc atcaagctgg ggcgcacgca
     6721 attgcaggac gcggtgccca tgactctggg gcaggagttc agcacctacg cggtgatgtt
     6781 gggcgaggac cgtatgcgac tgcgcgaggc ttcgcgtttg atcgaggaaa tcaacctggg
     6841 ggccaccgcg atcggcaccg ggatcaacac cgtgcccggc tatgcgcgcc tcgtgcgcag
     6901 ggaattgact cgaatctccg gggtcaaact catcaccgca cccaatctcg tggaagcgac
     6961 ccaggacgcg ggggctttcg tccaactctc tggcgtgctc aagcgcatcg ccgtaaaact
     7021 atccaaggtc tgcaacgatc tgcgcctgtt gtcctcagga cccatggcgg gattcaacga
     7081 gatcaatctg cccccgatgc aggcgggttc cagcatcatg cccggcaagg tcaacccggt
     7141 gattccggag gtcgtgaatc aggttgcctt tgaggtcatc gggcacgatg tcacggtgag
     7201 ttttgccgcg gagggcggac agctgcaact caacgccttc gagcccatca tcgcccatag
     7261 cctgttcact tcggtgtctc acctgcggcg cggctgcgta gtgttagcgg agcgctgcgt
     7321 caagggaatt accgcgaatc agaaacatct ggccagaatg gtcaaggact cgcctagtat
     7381 cgccaccgcg ctcaatcctt atatcggtta cgagaaggcc acctacatcg cgaagaaagc
     7441 gacaacaacg ggtcagtctg tctacgacct ggtggtgaat gaggggctct tgtcccgaaa
     7501 ggaggtggac aagatcctca ggccggaagt gctcacgcag ccgcagcccc tccgagcccc
     7561 ttcgatgaga cgccgaagca ctaaacgccc gaacgcacgc tgagcgcccg ggctttcgca
     7621 aacttgtgca tcatgcactc atagttcgaa gcgaatgccc tgggccaatg gaagttcgcc
     7681 ggagtaattg atggtgttgg tggctcgccg catgtaagcc ttccatgcat ccgatccgga
     7741 ctcgcgtcca ccacccgtat ccttttctcc accgaaagca ccgccgatct cagctcccga
     7801 ggtgccgatg ttgacgttgg caatgccgca atccgatccc gcgctgctca tgaacagctc
     7861 ggcttcccga agatcctggg tgaagatgga cgaggacaag ccgtgccgcg cggcattgtg
     7921 cagttggata gcctcgtcga gcgtatcgta gggcatagcg tagagaatgg gtgcgaaggt
     7981 ttcctccagt acgatgtcgg tctggctgcg catcgccgcc agcgccgggc gaacgtaata
     8041 cccgccggga tactctgtat cgacacgctc accacctgcg atccaccggg catcgacatt
     8101 ggcccgatcc agtgcggcgc gcatgccagc ataggcgttc gagtcgatca acggacccac
     8161 cagcgtgtcc gaatccagtg ggttgcccac tttggcgcgc gcgtatagcg atttcaaacg
     8221 gccaagaaat ccatcgaaga gacttcgatg cacgaacagc cggcgcaggc ttgtgcaacg
     8281 ctggccggcg gttcccactg ccgagaacag gatcgcgcgc tcggtcaagg cgaggtccgc
     8341 cgacggtgcg acgatggccg cgttgtttcc gcccaactcc aggatcgtgc gcgcgaagcg
     8401 gggcgccaac tgagccgcga cggccctgcc catgcgcgtt gagcccgttg ccgaagcaag
     8461 tcgaatctcc ggttgatcca gcagaatgcg tccctgatcg ctcgcaccga atacgacttc
     8521 attgagctga tgcggggcat tcccgatttc ccgacaggtc cgctccagca gtgcctgcat
     8581 ggctactgcg gtgagtggcg ttttctccga gggtttccac agcatggtgt tgccgcacac
     8641 gagggcgagc gcggcgttcc aggcccatac agcgacaggg aaattaaagg cggtgatgac
     8701 cacgcctgga cccagtggat gccaggtttc catcatgcga tgacccggcc gctcactggc
     8761 gatggtaagc ccatgcagct gccgcgagag cccgaccgcg aactcacaga tatcgatgac
     8821 ctcctgcacc tcaccgagac cttcctggag aatttttccc gattcgatac tgaccaacct
     8881 gccgagcgcc gctttgttgt tccgaaccgc ttcgccgaag aggcgaatga gctcgccgcg
     8941 cttgggcgcc ggtgtcatgc gccagtgccg gaaggcctcc cgcgatcggg caaccttttc
     9001 tcgaatactc gcttcgtcgt cgagcacgac cccgccgatc cgcgaaccgt caatgggtga
     9061 gcgggcgcaa agaccttccg tggagtcgcc ggcagaggcg ccaagggcat tcagcagaat
     9121 ctgtacttcg tgatgtagat tcatgtcgcc ctcatgtggc cgcggcctcg taggcgcggc
     9181 caaagcgatt ggacagaaaa tcggtaagcg cgacctgctc ctgccgaagg aaaccggatt
     9241 gcggaagttt gccctggacg aaaagatcca tcacggcgca tacaccgctg gcggtggtaa
     9301 tctggatcgc tgtcatgggc attccagacc aggtctgtgc atacactttg cgggcgaaag
     9361 acacctgagt gcgtttcccg cctcgcaagc ccgacgccgt cacgaacacc acgaccacat
     9421 cctgctcggt agcaggaatg gctgtttgca gtatgtcttt catggtctcg cgttttcctg
     9481 cgagcccgaa gtcttgcagc aacagcttgg cgatatcgcg gtggcccggg tagcggatcg
     9541 tcttatagtc gagatactgc acgtgacctt ccaaagtctc ggcaagagta cccaaaccgc
     9601 ccgaggtatt gaaggcttcg taattcacgc cgtccaacgc gaaggtctcg tagccttcca
     9661 gcgccggtag ttcgatgcgc cttccgtcca ctatggcctc gcagggatga acgtactcgt
     9721 tgatgagccc atccacggac caggtgaggt tgtacttcag cgcattagtg ggatattgcg
     9781 gcagggcgcc cacccgcatg tgcagccgtt ccagtttgtc gaagccttgc gacagcgcat
     9841 agcccgcaat cccaatgaag cccggcgcca gtccgcattg cggcatgaag gcaatggggg
     9901 ctccctcggc caacgcgcgg atagcacgcg tggtggagac gtcctcggtc aaatcaagat
     9961 agtgtgcctt ggcccgcgcg gcggcattgg ccaccgtatc ggcaagatgg aagggcaggg
    10021 cattgataac gatgtcatgg ccttggatac ggcgcgcaaa gctgccgata tcgtcgccat
    10081 cgatctcgaa ccagcgatcc gagaacaggg agcgtagctc gggatctacc aagcggtcgc
    10141 cgaccgcgac ttcgtaacgc ggttcgccct cggctgtaac gagcagatgt gcgacggcgc
    10201 ggccaatgtg gccggtgcca agcagcagaa ctcgtctcgc catggatcgt cgctccttgt
    10261 ttatgggaga actcgggaat cgggaagcaa gcttagttta ggccacactg cgccatgtca
    10321 gtgggattgc ccggcccagg tagccgtcac ggccacagcg ccgcggcatt cggatgttgc
    10381 gccgcaacgc ccggtatctg catccgatca cccgttgttt gttgccttcg gggatgtcgg
    10441 atcagcgcgt ccccgatact atgtcgcctc tggagcggag cggacggcgg accgggtgtt
    10501 cacgaaggcg ccgccgcttc ctggcgcaaa tcgaattgac gggggaatgg caatggaaca
    10561 ggaaatgttc ggattcgtcg gtgtgggaaa aatggggtcg ctcatgtcct cgcggctgct
    10621 caacgcggga cagcgcgtgt gcgtgttcga taccgatgag aaggccgtcg ccgcgctgga
    10681 gaaggccggc gcgcaaaggg ccggctcggc ggccgaagtg gccggcattg cacagaccgt
    10741 cttcctcagc cttcccaatc cggagatcgt gcagaacgtt gcgctgggcc gcggcggcgt
    10801 gatcgaggga acgcgcgtca agcacgtcgc cgatttctcc accatcggac cgcgcaccgc
    10861 gggcatcgtc gcgagggcgc tcgccgagcg caagattacg tacgtggatg caccggtgag
    10921 cggcggcctc aggggcgcgc gcgaaggcac gcttgcggtc atggtttcgt gtccgagaaa
    10981 tgtgtacgcg gtacttgagc cggtgctcaa gaccttcggc aagccctttc atctcggcga
    11041 ggtcgccgga caggcgcaga ccatgaaact cgccaataat cttttggcgg cggccgccat
    11101 tgcggtatct tccgaagcga tggtgatggg ggtgaaagcc gggctggatc cgcaaatgat
    11161 gctcgacatc ctcaatgccg gcaccggccg caacagcgcg acccaggaca agttcccgcg
    11221 cgccatcctg ccgggcacct tcgatttcgg tttcaccacc ggcttgtcgt acaaggacgt
    11281 gcggctttgc atcgacgaag ccgagtcgct aggcgtgccg atggtggtcg gcgcagcggt
    11341 gcgccagatg ctggcggtga ccaacgcggt ttacggtgcc gattcggatt tcacctcgat
    11401 gtgcagggta gtcgagtcgt gggccggcgt ggaagtgcgc agcaagtgac ggggagaatg
    11461 caatggtaac cgtacatccc gtgaaaaggg gcccggcggc ccagaccggc gagccgctcg
    11521 cagcgcgcat cgcgcactcg gtcgtctcga tgtcgctgaa cgacctgccc gccgaagcaa
    11581 ccgacaaggt gaagctctgc ctgttcgacc tcatcggctg cgccttcgag tcgcgcgatc
    11641 tgccgtggag ccggcaggcg gccgcgctgg cggaaggaac gggcggcgcc gcgacggtga
    11701 tcggatcgcc gcaccccgcg tcccacgccg atgcggcgtt cgcgaacgcg gtgatgggcc
    11761 acggcctggt gcgcgaggac atgcactccg gcagcatcag ccacctcggc atcgtggtgc
    11821 tgcccgcgct gctggcgctt tccgagcttc gccatgtgcg tggacgcaac ttcatcaccg
    11881 ccgcagtggt cggctacgaa gtcggcgggc agatcggccg cgcggtcatg gacgccgaag
    11941 tggcgcgcat ccaccgtccc accggaatca ccgggccggt cgccgcggct gccgcgggag
    12001 cgcgtctgct gggcttgagc accgacgcgg taatcagcgc gatcgcgctc ggcgccaaca
    12061 ctacggtcgg tttcaaccag tgggcgcata ccggcgggag cgaaatgttc ttccacgccg
    12121 gctacgctgc ccgcgacgcg gtgaccgcgg cgcgtctggc cgaaatgggc gcgttcgcat
    12181 cgccctcggc gctggacggc gaagccgggc tgttcgccgc gctgggcaag cgtccggctg
    12241 cggcaaagat cgcgttgttc cagggccagc cggaaatcct gtccgtttat cacaaacccg
    12301 ttcccgcctg taatttcgcg cagaccgcca gccaggcggc gctcgcgatt gcccacgaac
    12361 acaagcccaa gcctgagcgc atcgccgcca tcacggtgcg cgtgccgcgc gccggcgcgc
    12421 tctatcccgg ctgcgactac accggaccgt tcgcgcacgt gctgcaggcg aagatgagca
    12481 tccagtacaa cgtcgccgcg gctctgatcg aaggcggcgt gtccgagcag aacttcacgc
    12541 ggctgcagga cccggggttg cagcggctga tcggcgtcat gaagctggag atcgacgagg
    12601 cgatgaccag ggcgtatccc ggaaaacagg gcggcgaagt ggaagtgcgc gagagcggcg
    12661 gtgcgacgca tcgcatccgc ctggaggacg tcgtcaacgc gagtgccgcc gacgtgcggg
    12721 caagattccg tgcggcggcc gaaaacgtgc tcggcgcggc gcgggcgagc gaaatcgagt
    12781 ccttcataga cggcatcgaa ctgcgcaagg acgtcgcgca actcggcgcg ctgctccgcg
    12841 cccccgcaac ttagaaattt ttcaccacag agacagagag gtgcagagaa gtgcaaacaa
    12901 aagaagaacg cttggtcccg acgtgaaatc agatatgtcc cggcattctt tgttggattc
    12961 cctccgcgtc tctgtgtctc tgtggtgaag gaagtgagcg tcctggcatg actgctgcgc
    13021 agttgctgga aaacttcctc caggccctga ccgcgggcct gctgaccggg gcaatctatg
    13081 gcctgatgtg cgtgggcctg agtgtgatct tcggcgtcat gcgcgtcatc aacttcgcgc
    13141 agggcgactt catgatgctc ggcatgtacg tcgcctatta cgcgttcggc ctgttcggcg
    13201 cacatgccgt gttcggcacg gcgatggatc catacatcgc ggcgctgctc gccgggcctg
    13261 tgatcttcgt cgccggcgtc atcgtgcata agctgctggt gagccgggtg accggcatcc
    13321 gcggcagcgc gctcgagtcc gaggggcatt acgcgcagtt gatcctcacc ctcggcgtgg
    13381 cgctgatcct gcaaaacggc gggctgtacg tgttcggctc gacgccggtc tcgatcgcca
    13441 cgccactttc atcgagcgct tgggcgctgg gtccgctcta ccgcgacgcc accgttttcg
    13501 tgaatcaggg gcaggccgtc gccgcggtga tcgcggtggt ggcggtgctc ggattcgtgc
    13561 tgttcatggc caggtcgcgg ctcggcaagt cgctgcgtgc ggccgccgac aatgccgaag
    13621 cggcgatcta catgggcatc gacgtcgatc gcgcgcactg cctcgctttc ggcttcggcg
    13681 tgggcatgac cgcaatcggc ggcgggctgc ttgcatccgg cggtccgttc cagccgtacg
    13741 tcggtctcga gtacgtgatc gtcatgtacg ccggcgtcgt gctcggcgga ttgggcagcg
    13801 tgtcgggcgc ctttttcggc ggactgacga tcggcctggt ccagcagtta tcgacgctgt
    13861 tccttcccaa ccagttgcag aacaccgcga tcttcgtggt gttcctggtc atcgtgctgc
    13921 tacggccgca aggcctgttc ggcagggcgg cacgcaggac atgaagaggc gaacctgaaa
    13981 ataaccggga tgaatctgcg ccgccaggcg ctgctgatcg cggcattcgg catcgtctat
    14041 gccgtagcga ccgccttcgc ggagaactcc tactaccagt tgatcatgac ggtggttccg
    14101 gtgtgggcgg tgatggggct ggcgtggaac gtcttgagcg gctacagcgg tctggtgtcc
    14161 ttcggccatg cggcgttctt cggcctcggc gcgtactcgc tggcgctggc gcagatccac
    14221 tggggcctgt cgccctggct ggggattccg ctggcgggga tggtgggcgc gctggccggg
    14281 ctggtggtcg gtttccccac gtttcgactg cgcggtcatt actttgcgct ggcgatgctc
    14341 gcctatccgc tggcgctgct gtacgtgttc gagtggctgg gctaccagga gctgacgctg
    14401 ccgatgcaac gccaggcgcc gctcgcgtac atgcagttcg aggaccaccg catctacgcg
    14461 tggctggcgt tggcgctgat gcttggcgcg atggtgctga ccggcctgat cgagcgttcg
    14521 cgcttcggca tgtcgctgct cgcgatcaag caagacgaag ccgcggccga ggccgcaggg
    14581 atcaggacgc tggcgtggaa actcagggcg atcacgctga gcggcgcgat ggccggcacg
    14641 atcggcggtt tctacgcggt ggtgctgctg gtggtgacgc cgcctgcggt gttcggcatg
    14701 ctggtgtcgg cgcaggcgct gatcgtcgcc atgttcggag gcgtgggcac tctgtggggg
    14761 ccggtgatcg gcgcggtgat ccttgtgccg ctgagcgaac tgctgcatgc gaagctgggc
    14821 aacgtcatac ccggcatcca gggcgtggtc tacgggatcg cgatcgtcgc agtcattttg
    14881 ctggcgccag aaggcgtgtt ctggaaagtg aaggacctga tgttgcgccg gcgtgtgggc
    14941 aaaaatctcg accagccgca gtccgccggc ccggcatcgg cggtcgtcgc ggaactgccg
    15001 ctgtgcaaga agggcggcga cggcgcagtg gtgctcgagg tgcgaaatgt ttcgaaagcg
    15061 ttcggcggcc tccaggcggt gcgcgacgtc agcatgaagg tcgtgagcgg agagatcctc
    15121 ggcatcatcg gcccgaacgg cgccggcaag actacgttct tcaatttatt gaacggtttc
    15181 atccgccctg accaggggca gatcctgctc gagggcaggg acatcgtcgg cgcgcgcccg
    15241 tacgagatct gcgccgccgg agttggccgc acgtttcaag tagtcaggcc gttccggcgc
    15301 atgacggtgc tggaaaacgt cgtcgttggc gcgtacgtgc acgcggcctc cgaccgggag
    15361 gcgcgcgcca atgccgaagc cgcactcgcc cgggtcgggc tcgaccgcga cgcgcagctt
    15421 ctcgccggca gtctgtcgaa caagcaattg cgcctgctgg agctcgcccg cgcgctggcc
    15481 ggcagaccga aggtgctgct gctcgacgaa acgctggcgg gactgggggg cggcgaagtt
    15541 gaggaagtga tcgcgctggt gcgcaagctt gccgcgcgcg gcgtcaccat cgtcatcatc
    15601 gagcacacca tgcaggcgat ggtgcgcctg gtggaccgct tcgtcgtgct caacgatggc
    15661 gctgtgctgg ccgaggggca acccgccgac attacccgta atccggcggt gatcgacgcg
    15721 tacctgggaa agcgctggag agtcgccaat gcttgagatc gaggcccttg gcgcaggtta
    15781 cggcgccgtg ccggtgctga acgatgtcaa catacgcgtc ggcgccgggg aatttgttgc
    15841 gatcgtcggg cccaacggcg ccggcaagac cacgctgttc aagaccattt ccggtatcgt
    15901 gccgccgatg tccggcgtca ttcgcttcga gggcagggac ctgctggcgg ttgcgccttc
    15961 cgcgcggccg cacctgggca tcgcccacgt tcccgagggg cgccaggtgt ttccttcgct
    16021 gacggtgctc gagaacctgg aaatgggcgc cgccaccgag accggacgcc gcaactgggc
    16081 ggagaatctg gaaaaggtct acacctggtt tccggtgctg gcggaacgcg cgaagactca
    16141 ggcgggagcc ttgtccggcg gacagcagca gatgctggcc attgccaggg ggctggcgtc
    16201 gtcgcccaag ctgctgatgc tcgatgagcc gtcgctggga ttggcaccgg cggtcgccga
    16261 cgaaatcttc gagcgcatcg tcgccatcca cgccggctcc gggctcaccg tgctgatggt
    16321 cgagcaacgc gttgcggagg cgctgcactt cgccactcgc ggctatgtgc tggaagcggg
    16381 gcgcgtggtg ctcgaaggca gccatgacga actcaaaagc aacgaccgca tcaggcaggc
    16441 gtatcttgga atgtaactga gtcggtttcc tcgcagtcga acggaaccgg gggaggatga
    16501 aatgcacaac atgaaaataa acgcgctcgg aaggcacgcc gtccgcgcgc tgctcgctgc
    16561 ggcgatggtg gccgccgcga tcacggcgac cgggcagacg aaggaagtgg aggtcgggct
    16621 gatcgcgccg atgtcggggc cttacgcccg gcagggccag gtgatgaaga tggccgccga
    16681 catggcgatc gaaactgtca accagcaggg cggtatcaag tccatgggcg gagcgaaact
    16741 caagctatcg gtgttcgacg cgggcgattc ggtcgaaagg gcgaaaaatg cagcccagcg
    16801 catggtggcc aatgcgcccg acctggtggt cgcaaccggc gcgtatctca gctccttcac
    16861 gctggcggtc accgaagtca ccgagcgcgc cgagttgccg gtgatcacgt tctcgtactc
    16921 ggacctgatc acctcgcgcg gcttcaagta cgtgttccag acttccgcca ccggcgtgca
    16981 gcaggcggaa gggtcgctgc cggcgctgat ggagatggcg gagaaggcct ccggcaagcg
    17041 gccgcagacc atcggcatcg tcatggacaa caccgccgcg tcggtgtctt tcgtcaagcc
    17101 gatgcgcgac caccagttcg ccaagctcgg attgaaactg gtggtggacg agactttcac
    17161 gccgccgctg gcgaacgcca cgccgctgat ccagaaggtg cgctcggcgc ggccggacat
    17221 cctcatcctg ttgcccacgg tgatttcgga cgccaagctg ctgctggaaa aaatgaacga
    17281 gttcgggctc gggcgcggca aattgccaac catcgcaaat ggcgctgcga tcctcgaccc
    17341 ggacctgcgc aacaacatga acatcgagca actggaaggc gtcatggcag tggttgccaa
    17401 ctggacgacc agcgcgcaga aggatttcgt cgcggaagtg aaggccaaga ccaacgagcc
    17461 gtggccgacc cagcatttcg tgacgaccta tggcgacata ctgatcttca aggcggcgct
    17521 ggaagccgcc ggcaaagccg accggcaggc ggtggcccag gcgctgcgta cgatgaacct
    17581 cagcggcggc gcggcgaatt tcttccccgg caacggcaag ctgcggttcg aggagaacgg
    17641 ccggcgtgct gatgcgagcc tgttgatcgt ccaatggcag aagggtgtgc cgatcaccgt
    17701 ctatccgccc aaggatgccc tcgccgggcc cgcctggcct acgcaatagc gtgcggcgtt
    17761 ttttttagac agagcaccgc tcaccacgga ggacgcggag gacacggagg aagggcagga
    17821 gaaaaagcac ttgagaggta aaaccttcaa ttggcgaaca atccaccaaa gcagaaatta
    17881 gcagcagagg gtgacaacag ccgccttgct tttaacccca aatcgccttt cctcggtttt
    17941 gtctttcctc cgtgtcctcc gcgtcctccg tggtgagcgg tgctctgtct aaaaaaaaac
    18001 gccgcacgct attgcgtagg
//
LOCUS       DASQOP010000180         3656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2291297, whole genome shotgun sequence.
ACCESSION   DASQOP010000180 DASQOP010000000
VERSION     DASQOP010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2291297"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..567)
                     /locus_tag="VE030_07375"
     CDS             complement(103..567)
                     /locus_tag="VE030_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HZF23268.1"
                     /translation="MPRDNPIGPESRLRLAPLGIRLLSLAYEGVLLFGIVFVAAYLFL
                     GLARDAQSGLPRLVFQVYMLAVCGTYFVFCWARSGQTLPMKTWHIRLVTEQGKPLTVD
                     RAFRRYLLALPGVVSGLGVLWAFIDRDRQFLHDRLAKTRIVKDEGTRMKDEG"
     gene            complement(573..1022)
                     /locus_tag="VE030_07380"
     CDS             complement(573..1022)
                     /locus_tag="VE030_07380"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HZF23269.1"
                     /translation="MLVSLLYGDPVHAGEVHAQAGAPSWAQLSPEQQKILAPLQQEWD
                     RLEPDRRKRWLDLAARYPNLPAERQLRMQQRMQEWASLTPEQRAQARERYKQLKQLPP
                     EKRQQLHQRWQEYQHLPEEKRQRLREKRAPGPADPNAGKPGDQTSPR"
     gene            complement(1022..1411)
                     /locus_tag="VE030_07385"
     CDS             complement(1022..1411)
                     /locus_tag="VE030_07385"
                     /inference="COORDINATES: protein motif:HMM:NF023699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HZF23270.1"
                     /translation="MNENDLARRIVARLDRSLAELPTSTAQKLEAVRAAALARYRPGS
                     SPPGRSWVDRLVGSQGLRQGLAVRLVLPVAIVIASLAGLIYWQTSSHFEEELDTGLLA
                     GELPLHAYIDPGFETWLTHTSHTPPQQ"
     gene            complement(1408..1974)
                     /locus_tag="VE030_07390"
     CDS             complement(1408..1974)
                     /locus_tag="VE030_07390"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZF23271.1"
                     /translation="MATQQELAAFLADVERRAFKQAVFAVRDQHVALDIVQDAMLKLV
                     DKYAGRPAVELPLLFQRILQNTVRDFYRRQKVRSLWTTLFSSFHPRDEAEDYDPLETM
                     ETDGPGGRGDSPADRLERTQIGNLIGQALEKLPPRQRQAFLLRYWEELDVAETAAAMG
                     CSEGSVKTHCSRATHALAVFLKKKGIEP"
     gene            2159..2725
                     /locus_tag="VE030_07395"
     CDS             2159..2725
                     /locus_tag="VE030_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HZF23272.1"
                     /translation="MQSVNLTNHFLIAMPNMADPHFARSLTYICEHNDQGALGVVVNR
                     PTDMTLGSLFEQIEIPLKNQTLGSLPVYFGGPVQVDRGFVLHRPVGGWQSTLSVNGEV
                     GLTTSKDILQALSHGDGPGADNILVSLGYAGWAPGQLEHELGQNAWLTVRAEPSVMFD
                     RPPEERVAAAMELLGINPASLSEDAGHA"
     gene            2759..3229
                     /gene="ruvX"
                     /locus_tag="VE030_07400"
     CDS             2759..3229
                     /gene="ruvX"
                     /locus_tag="VE030_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HZF23273.1"
                     /translation="MESQRPAFPHAAHSTEHPQFGTVLAFDFGERRVGVAVGELQLGI
                     AHPLETIDAAANDARFARIAALVQEWRPVLLVVGLPLALDGGEHRLTGLARKFAQRLR
                     GRFGIETRLVDERLTSVEAQRETRASGLTARDSKRHVDPLAAKLILEALFNENA"
     gene            3216..>3656
                     /gene="pyrR"
                     /locus_tag="VE030_07405"
     CDS             3216..>3656
                     /gene="pyrR"
                     /locus_tag="VE030_07405"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HZF23274.1"
                     /translation="MRMPDAEALLDQLAERMRPQVSADAALIGIYTGGVWLAERLHRT
                     LGLKQPLGTIDVSFYRDDYQSRGLTARVKPSDIPFEVADADVILVDDVLYTGRTIRAA
                     INELFDYGRPRRIRLAALVDRGGRELPIAPQFLGAEVKVADNEQI"
BASE COUNT          665 a         1177 c         1146 g          668 t
ORIGIN      
        1 gcagacagag aaccaggaat tgctgaaaat cttcgtcacg atcgcaaagt cttccaagaa
       61 acaatccaga aaaggtacca agtgaaagcc tgtcttcatc cttcatcctt catccttcat
      121 ccttgtccct tcatccttga ctattcgtgt ctttgccagc cggtcgtgca ggaattgccg
      181 gtcgcgatcg atgaaagccc aaagcacgcc gaggcccgaa actacgccgg gcagggccag
      241 caggtagcgc cggaacgccc ggtcgaccgt caacggcttg ccctgctccg tgaccagccg
      301 gatgtgccag gtcttcatcg gtagcgtctg tccgcttcgc gcccagcaga atacgaagta
      361 agttccgcat accgcgagca tgtagacctg gaacaccaga cgcggcaggc cggactgtgc
      421 gtcgcgtgcg aggccgagga acaggtacgc cgcgacaaac acgatcccga acaacagcac
      481 gccttcgtag gcgagactca acagccgtat tcccaagggc gccaaccgca accggctctc
      541 cggaccgatc ggattgtcgc ggggcacgga aatcaacgcg gtgaagtctg gtcgcccggc
      601 ttaccggcat tggggtccgc cggtccgggg gcgcgcttct ccctcaggcg ttggcgcttt
      661 tcctcgggca agtgctggta ctcctgccaa cgctgatgca gttgctggcg tttttccgga
      721 ggaagctgct tgagctgctt gtagcgttcg cgggcttgtg cgcgctgttc cggagtcagc
      781 gatgcccact cttgcatgcg ctgctgcatg cgcaactggc gttctgccgg caggttcgga
      841 tagcgcgctg ccaggtcaag ccagcgcttg cgccggtcgg gctcaaggcg atcccattcc
      901 tgttgcagcg gcgccagaat tttttgctgc tcgggcgaaa gctgtgccca cgacggtgcg
      961 ccggcctgcg catgcacttc accggcatgc accggatccc cgtaaagaag cgaaaccagc
     1021 actattgctg cggcggtgtg tgcgacgtat gcgttagcca cgtttcaaag ccggggtcga
     1081 tataggcgtg taacggcagt tcgccggcaa gcaggccggt atccagctct tcttcgaaat
     1141 ggctcgaggt ttgccagtag atgagcccgg ccaggctggc aatgacaatc gccaccggca
     1201 gcaccaggcg cacggcaagg ccttgccgca gaccctggct gcccaccagt ctatccaccc
     1261 aggaccggcc gggcggcgaa ctgccggggc gataccgcgc cagggcagcc gcccggactg
     1321 cctccagctt ttgcgcggtc gaggtcggca actcggccag gctacggtcg agtcgcgcaa
     1381 cgatccgcct ggcaagatcg ttctcattca tggttcgatt cccttcttct tcaggaagac
     1441 agcgagggca tgggtggcac gcgagcagtg cgtcttcaca ctgccctccg agcaccccat
     1501 cgccgccgcg gtttccgcta cgtccaactc ctcccagtaa cgcaaaagaa aagcctggcg
     1561 ttgacggggg ggcaactttt caagggcttg tccgatcaaa ttcccaattt gcgtgcgctc
     1621 caggcgatcg gccgggctgt cgcccctgcc cccgggaccg tcggtctcca tggtctcgag
     1681 cgggtcgtaa tcctcggctt cgtccctggg atgaaacgag gaaaacagcg tggtccacag
     1741 cgaccgtact ttctggcgtc ggtagaagtc gcggacggtg ttctgcagga tgcgctggaa
     1801 cagcaggggc agctccacag ccggtcttcc tgcgtacttg tcgaccagtt tcagcatggc
     1861 gtcctggacg atatccagcg cgacgtgctg atcgcgcacc gcaaacacgg cctgtttgaa
     1921 cgcccgccgc tctacgtccg cgaggaacgc ggccagttct tgttgggtag ccagaaatgt
     1981 ccccgagcct ctttggaaaa tcgctggccg atgcgttgcc ggccggtgca ggcaagcctg
     2041 cgcaaccggc catatccgct ggtcctgccg gccgattctc aagacctgat tataccgggg
     2101 cacgcagccg ggtttgtggc ggccatgccg gggcgggtaa aatggcgctg gcgcatccat
     2161 gcaaagcgta aatctcacca atcacttcct gatcgccatg cccaacatgg ccgatcccca
     2221 ctttgccaga tcgctgacct atatctgcga acacaacgat cagggtgcgc tcggggtggt
     2281 ggtcaatcgc cccaccgaca tgacgctcgg ctccctgttc gagcagatcg agattcctct
     2341 gaagaaccag accctcggga gcctgccggt ttacttcggc ggcccggtac aggtggatcg
     2401 cggtttcgtc ctgcatcggc cggtgggggg ctggcagtcc acgctgtcgg tgaacgggga
     2461 ggtcgggctg accacctcca aagatatctt gcaagctttg agccatggcg acggccccgg
     2521 cgccgacaac atcctggtgt ccctcggtta tgccgggtgg gcacccgggc aactggagca
     2581 cgaactcggg caaaacgcct ggctgacggt gcgcgccgag ccctcggtca tgttcgaccg
     2641 gccgcctgag gagcgtgtgg ctgcggcgat ggagctgctt gggataaatc ctgccagcct
     2701 ttcagaggat gccgggcatg cgtgacgtga attctgcggg cgcacccgag tgaggccgat
     2761 ggagagccag cggccggcgt ttccgcacgc agcacacagc actgagcacc cacaattcgg
     2821 caccgtactt gccttcgact ttggcgaacg tcgcgtcggt gtagctgtcg gggaactgca
     2881 gctcggcatc gcgcacccgc tcgagaccat cgatgccgcg gccaacgatg cgcgctttgc
     2941 ccgcatcgcc gcgctggtac aggaatggcg gccggtactg ctggtggtgg ggctgccgct
     3001 ggccctggac ggaggcgagc accggttgac cgggctggcg cgcaagtttg cgcagcggct
     3061 gcgcggccgc tttggcatcg aaacgcggct ggtcgacgaa cgtctgacct cggtggaagc
     3121 gcagcgcgag acgcgcgcga gcggcctgac tgcccgcgac agcaaacgcc atgtggatcc
     3181 gctcgccgcg aaactcatcc tggaagctct tttcaatgag aatgcctgac gccgaagccc
     3241 tgctcgatca gcttgccgag cgcatgcgcc cgcaagtcag cgcggacgcc gcgctgatcg
     3301 gaatctacac cggcggcgtg tggctggccg agcgcctgca ccggacgctg gggctgaagc
     3361 agccgctggg aaccatcgac gtgtccttct accgcgatga ctaccaaagc cgcgggctga
     3421 ccgcgcgggt caagccttcc gacataccct tcgaagtggc cgatgccgac gtgatcctgg
     3481 tcgacgatgt cctctatacc ggccgtacca tacgtgccgc gatcaacgaa ctgttcgact
     3541 acgggcggcc gcggcgcata cgcctggcgg cactggtcga ccgcggcggc cgcgaactcc
     3601 cgattgcacc ccagttcctc ggcgccgagg tcaaggtcgc ggacaacgag cagatc
//
LOCUS       DASQOP010000181        31335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_883934, whole genome shotgun sequence.
ACCESSION   DASQOP010000181 DASQOP010000000
VERSION     DASQOP010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 31335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31335
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883934"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1224)
                     /locus_tag="VE030_07410"
     CDS             complement(<1..1224)
                     /locus_tag="VE030_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6513 domain-containing protein"
                     /protein_id="HZF23275.1"
                     /translation="MPEHLLFLTGRLAEKSLHRVLESMQPAPFSHAVHVLSIQVAGLM
                     TSDMIRRRLPDALGVNRVIVPGRCRGDLEPLSAHFGVPFQRGPEDLKDLPRFFGREGK
                     PLDLSKYDVRIFAEIVDAPNMSIEVILEQARACRDSGADVIDLGCLPKTPFPHLEESV
                     AALHAAGHRVSVDSMDEDELLRGGRAGADYLLSLKNDTLWIADEVASTPVLIPSKAGN
                     LRSLLRAIEAIDKKGRAYLADPILDPIHFGFTESLTRYQELRRRRPDLQIMMGTGNIT
                     ELTDADTLGMQALLMGIISELRITNILTTQVSAHASGVVQEANAARRMMYAAREADDL
                     PRAFGEALLALRSLDPFPYSAEEIATTAAAIRDPSFRIQISPEGLHVYNRDGLRTASD
                     PFKLFPHLGLEADGSH"
     gene            complement(1217..1768)
                     /locus_tag="VE030_07415"
     CDS             complement(1217..1768)
                     /locus_tag="VE030_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HZF23276.1"
                     /translation="MAKVKEPRIAWALTGSGHYLKECLDIVRTLSNVDLFLTKAAAEV
                     LKMYGYDLKKLKEDIHIYRDTTASAVPVTFLYEGNYHSVVIAPATSNTVAKCVAGISD
                     TLATNIYAQAGKCRIASIVFACDTAPELISEAPGGDVWVYPRPIDLENVGKLGKFEHT
                     TVVISIDELRIALKKRVERGRIA"
     gene            2047..2421
                     /locus_tag="VE030_07420"
     CDS             2047..2421
                     /locus_tag="VE030_07420"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HZF23277.1"
                     /translation="MEPAEKAYSEAEVKQRLAVELPHWYYEDGWIRRKFKTGGWKGTL
                     MVVNTVGHLAEAAWHHPDLTVSYAFVIVKLVTHSAKGITDKDLALAKKIEEVIQWQPG
                     KEGGVLEGTPNDDQRFKYIKYD"
     gene            2492..2842
                     /locus_tag="VE030_07425"
     CDS             2492..2842
                     /locus_tag="VE030_07425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HZF23278.1"
                     /translation="MDIIFVRDIRLETLIGAFEHERRQPRTLQVNVEIGRPAIRACKT
                     DQLADTIDYAAVVSVVQELFASHRFHLLEPLADTLADLMLSRFEAQWVRVEISKIGVV
                     PNARDVGVRIERSR"
     gene            3466..5508
                     /locus_tag="VE030_07430"
     CDS             3466..5508
                     /locus_tag="VE030_07430"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HZF23279.1"
                     /translation="MNHNQINTDVTAIRRAYGQFLEKGDVPEGVVPDFIKRSWQRSVA
                     FGVPYDRIREVGRIDETDLRVVVSQYGSLINLAAPVMENLHHQLTGSGSVVLLCSGEG
                     LILHSMGDPDFIPRAQHVALKPGVSWAEEQKGTNAIGTAIVEREPVVIYAGHHFIRQN
                     HFLACSASPVLDPNGDVAGVLDITCDYRGHQRHTIALVQMAVRVIERQMFQHRFCRDV
                     VFNVHPERNYLGSPFDVQVAFSPHGDYLAATPATCGQLDLRPGAFRGLFDELFDASYG
                     QVLRKLGSVSPPVIQVRLRHNGMPVYLQLAQPTVVAKTGHGTPVDSPAPKRQPSPDGQ
                     RTLHELRLDTLGASDPVTLFNIEKARRTLGRDIPILIEGETGVGKEWLARAIHNSGPR
                     AGGEFVALNCASISEGPLEAELFGYEEGAFGAKHKAATSKIQQADGGTLFLDEVGDMP
                     LNLQARLLRVLQERAITPVDGKPVNVDLAVICATHHRLHDLVRAGGFREDLYYRLNGL
                     TLFLPPLRERKDVLELATALAAKGCVAPRCIEFSDEVMDIFAHHPWPGNLRQMHSVIR
                     TALAMMDAGEKLGRRHLPEDFLEELTLAPAAAAAREALAAQAVMSSMYADSLEQIELQ
                     AIQETIRQCKGNISAAARQLNVSRTTLYRKLKNSSFATPDPDRRGAARKRGDDLHG"
     gene            5858..7654
                     /locus_tag="VE030_07435"
     CDS             5858..7654
                     /locus_tag="VE030_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol/ethanol family PQQ-dependent
                     dehydrogenase"
                     /protein_id="HZF23280.1"
                     /translation="MRVTLRYLVLPLALTAAAGVAQANSDVERLTANPKNWAMQAGDM
                     YNQRYSKLGQINAGNVNKLQVAWTFSTGVLRGHEGSPLVIGDTMYLHSPFPNRVFAID
                     LNTNKIKWKYTPKQDPAVIPQMCCDTVNRGLAYAEGKVFLQQADSTLVAFDANSGKVL
                     WSVKNGDPSVGAVNTNAPHVFKDKVITGISGGEWGVRGYVAAYNINTGDLVWKGYSTG
                     PDDEMLIDPGTTTTWMDGRVQPVGEDSSLKTWKEDLWKIGGGTTWGWYSYDKALNLMY
                     YGTGNPSTWNPAQRPGDNKWSMSVWARDVDTGKVKWVYQMTPYDEWDFDGINEMILAD
                     IPVKGKMTKALVHFDRNGFGYTLDRTNGALLVAEKYDPKVNWATHVDMKSGRPQVVSK
                     YSTAQNGPDVNTKGVCPAALGSKDEQPAAYSPKTRLFYVPTNHVCMDYEPFAVEYTAG
                     QPYVGATLSMYPPPGEKHMGNFIAWDAGTGKIVWSKPEKFSVWSGALATAGDVIFYGT
                     LEGYLKAVDNKGNELWKFKTPSGIIGNVFTYEHKGKQYVGVFSGIGGWAGIGMAAGLE
                     KDTDGLGAVGGYKELNQYTELGGSLTVFALGN"
     gene            7741..8154
                     /locus_tag="VE030_07440"
     CDS             7741..8154
                     /locus_tag="VE030_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZF23281.1"
                     /translation="MSTPMSGRRILVAVFAVIATLQAGADESAGSNLYTVVDGYKVDP
                     ATMKGFRAWRAAACDRCHGANQEGLVGPSLINSLKVLSKEEFVKTVTEGRLDKGMVSF
                     KTSKIVMDNLDNLYAYLKGRSDGAITRAKVEELKP"
     gene            8151..9050
                     /locus_tag="VE030_07445"
     CDS             8151..9050
                     /locus_tag="VE030_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated putative
                     ABC transporter substrate-binding protein"
                     /protein_id="HZF23282.1"
                     /translation="MTLCGSHTGVLQASQASNDRRGQGAIAFLAACVFVVVGAGAAAD
                     EPDEAERKVFKVCADPNNLPFSNQQGEGYENKLAELFGREMGLPVETYFQAQRLNFVR
                     NTLRFKLPGEDYRCDIMMGVPVGFEQVATTRPYYRSTYVLVYIKGRGLDAVNSVADFL
                     ALPREQLRRLKIGVFDRSPASQWLKNHDLVDSGVPYRMMNARPDYYPGEIVEKDLVQG
                     KVDVAVLWGPIAGFAATRVKDHEVVVVPMPSEPGVKFDYQVAMGVRHGETKWKQRIDG
                     LIESKLPQIREILQSYRVPLQDP"
     gene            9149..9973
                     /locus_tag="VE030_07450"
     CDS             9149..9973
                     /locus_tag="VE030_07450"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HZF23283.1"
                     /translation="MLTLYLLACASVWADPGRAQVLKLAPSLARVEANKTTGGYAFGT
                     AVAIAPDRVVTNCHVTREAQAIYVVKGGLRWDARAQLADVDHDLCLLWVPGLDARAAP
                     LATSASLHLGQPVAAIGYGGATGIQISIGDVISLHALDGGQVIRSSAAFSGGASGGGL
                     FDEDGRLVGILSFRLQARGAYFFSVPVEWFSERVLDLSRYQPVAPLGEARSFWERPQA
                     EQPYFMRATSLEAAHDWPELLRFTDTWVKAEPGNPDPQYFRDKALNAPLNGNRELQ"
     gene            9970..10335
                     /locus_tag="VE030_07455"
     CDS             9970..10335
                     /locus_tag="VE030_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23284.1"
                     /translation="MKMRLPVLLGFLGFAATAPAADAAGNDFPTVARVEYVQECMRNH
                     PGSRYEMLYKCSCALDRIAAEIRYDEYVELWTAANAVSIGGERGAQLRDAQMSQDMAK
                     KFRSIEGKAQKDCFLRKEE"
     gene            10332..11192
                     /locus_tag="VE030_07460"
     CDS             10332..11192
                     /locus_tag="VE030_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated SoxYZ-like
                     carrier"
                     /protein_id="HZF23285.1"
                     /translation="MTLGAMRRGMRKGCGPLLLGLGLALSMPAAQGAEAVPDTDIWLK
                     VRESFFGARPIRADADQVIRLEVPYRAQDAATVPIAIRTRIAQAGEPYIRTVYLVVDR
                     NPSPIAAIFRFTPDSGRAEIETRIRIEEYTPVRAIAEMSDGELHGSARFVKAAGGCSA
                     PAGKDPEAALARLGRMKFHLDQSVVLGAPNLVQLMISHPNHSGLAMDQLTHLYVPAYF
                     VRTVNVTYAGKPVLGAEVDFSISENPNFRFYFVPREEGELKAEVVDTKDLRFEYVLAV
                     RRGLSTKASE"
     gene            complement(11447..12367)
                     /locus_tag="VE030_07465"
     CDS             complement(11447..12367)
                     /locus_tag="VE030_07465"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HZF23286.1"
                     /translation="MATQLTFEGVYPILATPFDDNENLDLESFDRLVRFMAGLGVDGI
                     TVLGVLGEANRLLDSERAELIRTAVAAAGAMPVIVGASHSGTRAATELAQMAENLGAM
                     AVMVTPHAETVPNDDRIFEYYRSIAEGIRIPIVAQDHPASTQVHMTAALLLRIIRELP
                     QVASIKEEAVPTAPKIRALAAGMKERKVPILTGLGALYGLFDLQAGSAGFNTGFAFPE
                     VLMAMVKAARAGNWARAQEIYTRFLPLVVFEQQPGVAIRKEILRLRGGLKTSKVRHPG
                     AALQPATAEQLRKLIDALLPGQDLSRPVSL"
     gene            12490..13254
                     /locus_tag="VE030_07470"
     CDS             12490..13254
                     /locus_tag="VE030_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HZF23287.1"
                     /translation="MDMPVNQLKRALREGRPQVGCWLSLASHASAEICAGAGFDWVLV
                     DTEHAPADLPQVHHQLHAIAAYPVSALVRPYWNDTVVLKRLLDIGVQSFVIPYVQSAE
                     EAKRAVAAVRYPPRGVRGVSTNSRSNRFGRVKDYFAHADNEICLIVQVETRAALAEIE
                     KTAAIDGIDGLFIGPQDLAADFGHLGNPGHPEVQAAMAEAIKRIRKAGKAPGILAFAE
                     ADAKRWLELGALFVAVTSDQFLLARESAAVAARFKG"
     gene            complement(13537..14076)
                     /locus_tag="VE030_07475"
     CDS             complement(13537..14076)
                     /locus_tag="VE030_07475"
                     /inference="COORDINATES: protein motif:HMM:NF023113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3280 domain-containing protein"
                     /protein_id="HZF23288.1"
                     /translation="MSAIDRYVAFSVLFCLATPLAASETDAPAPRIAAVMEFELIDDM
                     RDYERPETRDAQNRRIGLISDALRRELLQRGMYRIADNSAAESLISGLKAQGELRECN
                     GCEIDIGRALSADVIIIGWVQKVSNLILNINIEVKEVSSGRMLYVKSVDLRSNTDNSW
                     LRGIRYMVDSIEEKKQHLR"
     gene            complement(14073..14588)
                     /locus_tag="VE030_07480"
     CDS             complement(14073..14588)
                     /locus_tag="VE030_07480"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HZF23289.1"
                     /translation="MIVVLLGVSGAGKTTIGELLSQRLGWPLLDADDFHPPANIEKMR
                     SGIALTDEDRWPWLDRLNGLLKEKDSNGQNVLLACSALKQKYRDRLAAGCSNLRWVHL
                     KGSFELIEWRLKTRKGHYMKAGLLESQFAALEEPIDAITVDVGAAPEAIADTVEAALN
                     GARMPAAGSRR"
     gene            complement(14585..15502)
                     /gene="gnd"
                     /locus_tag="VE030_07485"
     CDS             complement(14585..15502)
                     /gene="gnd"
                     /locus_tag="VE030_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HZF23290.1"
                     /translation="MDIGIVGLGRMGANMARRLARKGVRVAAHDVSPVARSAVEHERG
                     ITAHPELKNVIAALPAPRVVWVMLPAGEVTERALEQLALLLAKGDVLVDGGNALYKDS
                     QRRARWLAERDIGFVDAGVSGGVWGLDNGYALMLGGAKEAFTRVEPFARLLAPAPDHG
                     WTHCGPAGSGHYVKMIHNGIEYGMMQAYAEGLSVLKAKSEFGLDLAAITEMWRHGSVV
                     RSWLLDLIADFLRNERSLERIKPLVADSGEGRWTAIEAVELGVPAPVMSLALMARFAS
                     QGQDDYASKLLAMMRASFGGHPVHAAKSK"
     gene            15617..16078
                     /locus_tag="VE030_07490"
     CDS             15617..16078
                     /locus_tag="VE030_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HZF23291.1"
                     /translation="MARAAFRNLEELAAKVGQEVVVSDWLEVTQERIDQFADATGDHQ
                     WIHVDVERARRESPFGTTIAHGFLTLSLLSHFLNNSLEFGNSKMGVNYGCNRLRFTAP
                     VKAGSRLRARFKLKEFQRIEGGVQMIWDVAMECEGQQKPVLVAEWVGRRYG"
     gene            16071..16856
                     /locus_tag="VE030_07495"
     CDS             16071..16856
                     /locus_tag="VE030_07495"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HZF23292.1"
                     /translation="MAERLPAIFVSHGAPLLAIQPGPAREFLSGLGRTLGKPKAILTV
                     SAHWESGDAAVSAVARPETIHDFYGFPDELYRIEYPAPGAPELAVQAKALLGEKGIGA
                     RLHTTRGLDHGAWVPLMLMYPDADIPAAQLTVQTALGPAHHFRLGEALRGLRDQGVLI
                     LGSGGATHNLREFGRHPEGAAPPQWVTGFQEWLAQTIEGGKYDDLVRYRSLAPEAARN
                     HPTEEHFLPLFVPAGAGSPGVAGKRIHRSHTFGILAMDAYRFD"
     gene            16878..17330
                     /locus_tag="VE030_07500"
     CDS             16878..17330
                     /locus_tag="VE030_07500"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016254048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZF23293.1"
                     /translation="MARTDFRFAHRMRVRWAEVDKQGIVFNGHYLMYFDVAITEYYRA
                     LGYPYPEGLARHGTDLYVKKVEVEYQASAGYDDEIDVCVRVARLGRSSFEFLLEIYRA
                     DQLLVGGRVVYVNADPETRKSAALPEFLRSGIKAFEHTAPTEAVANPA"
     gene            17467..18681
                     /locus_tag="VE030_07505"
     CDS             17467..18681
                     /locus_tag="VE030_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZF23294.1"
                     /translation="MDFNFSDRVRDLQKKVGAFMGAHVYPNEHRFFEEIEENRRKGNA
                     WIPTRIIEDLKPKARAAGLWNLWRPKDHGGALTNLEYAPLCEIMGRLAWAPEVFNCSA
                     PDTGNMEVLLRYGTPEQQKQWLTPLLEGQMRSAFLMTEPEVASSDATNIQCRIERQGN
                     EYVINGRKWWSSGAGDPRCKIYILMGKTDPGNADRHKQQSMILVPADAKGVKVVRALS
                     VFGYDDAPHGHMDVTLENVRVPTTNLLLGEGRGFEIAQGRLGPGRIHHCMRIIGQAER
                     ALEFMCKRLKSRVAFGKPVAEQSVWHERIAESRIMIEQARLLTLKAAFMMDTVGNKEA
                     KAEIAMIKVIAPNMAQQIIDWAIQAHGGGGVCQDFPLSWMYAWNRTLRLADGPDEVHR
                     SQVARLELKKHA"
     gene            19227..19676
                     /locus_tag="VE030_07510"
     CDS             19227..19676
                     /locus_tag="VE030_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZF23295.1"
                     /translation="MKISFNVNGKQSSVDVPPDTPLLWVLRDTLGLTGTKFGCGKALC
                     GACTVHLNGMAVRSCQTPVGSVSGQKVTTIEGLGGNHKVQKAWIALDVPQCGYCQPGQ
                     MMSAAALLAANKKPTDDDIDAFMSGNICRCGTYPRIRAAIKEAAKMA"
     gene            19688..21889
                     /locus_tag="VE030_07515"
     CDS             19688..21889
                     /locus_tag="VE030_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HZF23296.1"
                     /translation="MENIIKNETRRDFLKVSGTVAGGLALGFYLPTGPRIAQAAVQTN
                     SPNAWIKIGTDNSVTIMCARSEMGQDVYTSMTMLVVEELEVDINKVKVEFAPPAEVYI
                     NALLGGQLTGGSTAVRDAWEKLRKAGASARMMLVAAAASEWGVDASQLKAANGMVTGP
                     GGKQATYGKLAAKAATMEVPKDVPLKPSSQFKVVGNVRQKRLDTAAKVNGTAQFGIDT
                     RVPGMLYAAVAMAPMIGGKVASYDDSRAKSVPGVKAVVQYSRGVAVVADSYWQAKKGK
                     DLLLIQWDAGPNADNDQKKIWEGIRNASQQPGAVFREHGDVAAGMGQAAKTVEATYQL
                     PFLSHSPMEPQNTTADVRADKAVIITPTQFQQLIPHVVAGATGLKPEQVEVHTTFLGG
                     GFGRRVEVDYAIDAAEISKAVGAPVKMVWSREDDMTHDSYRPAGLYKLTAGLDSGGKP
                     VAMRFHSTSPSISARLFPSVVKDGIDPFAVEGIDNYPYETPNLKFTYQMHDTGVTPGY
                     WRAVSHNLNAVALECFIDEMAQAAGKDPIQYRIDMLDMGSTKHQWSGLSAGVPVGTRM
                     KRVLEEVRAKSGWSKKMPEGKGMGVAVMEGYNTVIAMVAEVTVSSSYDVSVDKITAVI
                     DAGTLVHPDQALAQMQSTINFGQSACMWGEITIKNGGVEQTNFDMYRVARMNENPKVL
                     DIHFIKSDSVPGGLGEPGTAVVQPAIGNAIFAACGKRCRTLPFTPENIGGA"
     gene            22047..23075
                     /locus_tag="VE030_07520"
     CDS             22047..23075
                     /locus_tag="VE030_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HZF23297.1"
                     /translation="MDSVDLQVLRSSVTWLSQGHRVILATIVETWGSSPRPAGAMLAV
                     RGDGLVAGSVSGGCVEDDLIFRIRNKSLAVERPEIATYGISKDEATRYGLPCGGKLEL
                     VLEPIRDNDSVIDLLNRVDRHELVARTLDLDTGRVALRPASRADAPLSFDRKRLTTVH
                     GPRWRLLIIGAGQLSQYLAQMAQALDYSVTVCDPREEYADTWNVEGAVLERGMPDDVV
                     LAMGLDSHSAVVALTHDPKLDDLALMEALKSPAFYVGALGSRLNSQKRRERLTMFDLS
                     QGELDRLHGPIGLSIGSKTPPEIAVSILAEMTAVRHGVDPNGVGARKSGEVAADGNQQ
                     VCATVSRA"
     gene            23168..23923
                     /locus_tag="VE030_07525"
     CDS             23168..23923
                     /locus_tag="VE030_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZF23298.1"
                     /translation="MRLKNKIAIVTGAGSGFGEGIAKRFAEEGCKIVVNDINVEGGER
                     VAGEINAAGGKAAFFKGDVSRDADWKSMLKVALDTFSGLNIVVNNAGTTHRNQPLLNV
                     AEEQFDRVYAVNVKSLFHSARNIVPHFRQKGGGVFITVASTAGVRPRPGLVWYNSSKG
                     AAIVASRSMAVELAPDKIRVNVVNPVAGDTPLLVEFMGADTPEMRAKFVATIPLGRFS
                     KPLDVANACLYLASDEAEFITGACLEVDGGRCV"
     gene            complement(24144..25334)
                     /locus_tag="VE030_07530"
     CDS             complement(24144..25334)
                     /locus_tag="VE030_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZF23299.1"
                     /translation="MNAPLTRPSFRWDDPLFFDEQLSDEERMVRDSARAYCQEKLMPR
                     VVEANRHEKFDRAVLSEMGSLGFFGPTIEGYGCAGLNHVCYGLITRELERVDSGYRSS
                     MSVQSSLVMYPIYTYGTEAQRQKYLPGLAKGDLIGAFGLTEPDYGSDAGGMISRARKV
                     DGGYLLKGAKMWITHSPVADVMVVWAKDDRDVIRGFIVERGMKGLSTPKIEGKFSLRV
                     SVTGEIVMDDVFVPDGNLLPNVEGLKGPFGCLNKARYGISWGALGAAEFCWHAARDYV
                     LDRKQFGRPLAANQLIQLKLANMQTEITLGLQTVLRVGRLMDEGRAAPEMISLIKRNS
                     AGKALDIARLARDMHGGNGVADEYHVIRHAMNLEAVNTYEGTHDIHALILGRAQTGIQ
                     AFTA"
     gene            complement(25498..25740)
                     /locus_tag="VE030_07535"
     CDS             complement(25498..25740)
                     /locus_tag="VE030_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23300.1"
                     /translation="MKRLSLLLAAALLHACAVQKKEPNVNLGGYPPEFRAGYLDGCES
                     AKRTVGHKKDEQRFKREPQYVTGWHDGFDICGKQKK"
     gene            complement(25745..26581)
                     /locus_tag="VE030_07540"
     CDS             complement(25745..26581)
                     /locus_tag="VE030_07540"
                     /inference="COORDINATES: protein motif:HMM:NF033920.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA2778 family cysteine peptidase"
                     /protein_id="HZF23301.1"
                     /translation="MDQSPFFPQEEFQCGPAALATTLNAAGISAMPEDLARQVFLPAR
                     RGSLQVEMLAAARRHDALAVTLSPRLDALLAEVANGTPVIVLQNLALDWVPMWHYAVV
                     VGYDLAADQIILRSGRTRRQVMAMPTFERTWARSRYWAMVTVPPGTIPATVGESDYVQ
                     AAVAFEKVASATAARHVYSAATEKWPHNQLALLGLGNTAFSLQDWQAAETAFTKLADA
                     ASDPVPALNNLALTLDAQGQLTQALEVARRAVSLGGQFAAQSRNTLATLVEKTRDDGR
                     NR"
     gene            complement(26823..27371)
                     /locus_tag="VE030_07545"
     CDS             complement(26823..27371)
                     /locus_tag="VE030_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23302.1"
                     /translation="MDQKNRLLPLLTIAAAASVVAFSSIGIAAITGHLPIARSSLNPF
                     SAFGGAPAVPTSGSDRVPSSLIHEGLTRSAGETAAQGKPVSFRSGARLPARKPSCPNC
                     GVVDSIKAQQASDGDGKLAAALGTTGRAYQIDSEKLSSFSFVVIVRMENGTVRTIHEN
                     QRPPFSIGERVKLVNGTVIPLG"
     gene            complement(27668..29014)
                     /locus_tag="VE030_07550"
     CDS             complement(27668..29014)
                     /locus_tag="VE030_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HZF23303.1"
                     /translation="MSKARILIVDDDPDLLRLLTFRLEGAGYAVESADSAERALARLS
                     ASTPQLVITDLRMSGMDGMALFENIHKSNPALPVIILTAHGTIPDAVAATQRGVFGYL
                     TKPFDSKELLLQAEKAITLSGNHAEKATGNTDWRVEIITRSPAMESILGKAKLVAASD
                     ASVLIRGDSGTGKELLARAVHRASPRAEKPFVAVNCGAIPEQLLESELFGHMKGSFTG
                     AARDYKGLFQSAEGGTVFLDEIGDMPLPLQVKLLRVLQEKEIRAIGSTQTIPVDVRII
                     SATHRNLEDAIKIGAFREDLYYRLNVVSFALPSLAERREDIPVLAAYFLAQLADKYKK
                     PLNGFASEAMEILVKSSWPGNVRELYNVVEQAVALSTTQIIPPALVENAIRGQTAELS
                     SFEAARSEFEREYLAKLLKITGGNVTQAARLAKRNRTEFYKLLQRHHLDPKLFKAL"
     gene            complement(29017..29571)
                     /locus_tag="VE030_07555"
     CDS             complement(29017..29571)
                     /locus_tag="VE030_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23304.1"
                     /translation="MIRLFIVSFVVLATLIGGCAATTGPNTRRDPWYQVVVEPMQGND
                     ARALRFYENVLMLKGAELARELNTTRQEFDKDQSELNRVRLALLLSLPGTGFRDDQTA
                     LNLVQPFVNDKNYENSVLRALALMLHTQLSELKRLDEAAQQQTAKAKEEQRRADALQQ
                     KLEAILDMEMKMIEREQTTPPKRR"
     gene            complement(29568..31013)
                     /locus_tag="VE030_07560"
     CDS             complement(29568..31013)
                     /locus_tag="VE030_07560"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZF23305.1"
                     /translation="MQLYYPRSFLKLILAGFSLAVLPLIFALINNAFSIHQLATHSQR
                     AVYQAVQATQNSRFLIEQINNMERSVRQFAIIGEPSLLQGYGLAHERFVDTARRMATL
                     PLDARQKQALEALRAREAVLFDRIGQSGAKADVIRSAVAELAALSEVARALDEQGNLL
                     VDREVDTMQKMAGEVQRFIYWQLLALVPIALLLVIAATLLISKPIAQLESAIQNLGEG
                     RFDAPVSVNGPADLESLGHQLDWLRLRLLDLEEQKSRFLRHVSHELKTPLTALREGVE
                     LLSDEVTGKMTAGQFEIARILRQNTLRLQKLIEDLLNYHSVQFHTSALTFSQFDLKTM
                     VTRVAQQHSLTMRSKNLSLNLSASDLQIEADENKIEAIIDNLLSNAIKFSPVGGTIRV
                     QLRRRGADAVIDVMDEGPGISPEERGRVFEPFYQGNTVYTGPVKGTGLGLAIVREYAT
                     VHQGSAEIIDDGSPGAHLRVILPTRQMEAAA"
BASE COUNT         6106 a         9326 c         9916 g         5987 t
ORIGIN      
        1 atgcgaaccg tcggcttcga gcccgagatg cgggaacagc ttgaacggat cggaggcggt
       61 acgaagcccg tcgcggttgt acacgtgcag cccctcgggg ctgatctgga tgcggaagct
      121 cgggtccctg atggccgcgg cggtggtggc gatctcttcg gcactatagg ggaagggatc
      181 cagagaacgc aatgccaaca gcgcttcgcc gaaggcccgc ggcagatcgt cggcttcgcg
      241 cgcggcgtac atcatgcgcc gggcggcgtt cgcctcctgt actacgcccg aggcgtgcgc
      301 gcttacctgg gtagtgagga tgttggtgat gcgcagctcg gatatgatgc ccatcagcag
      361 cgcctgcatg cccagcgtgt cggcatcggt cagctcggtg atgttgccgg tgcccatcat
      421 gatctgcagg tccggccgcc gccggcgcag ctcctgatag cgcgtcaggg attcggtgaa
      481 tccgaaatga attggatcga gaatcgggtc ggcgagatag gcgcgccctt tcttgtcaat
      541 cgcttcgatt gcgcgcagca gagaacgcaa gtttccggcc ttcgacggga tcagcaccgg
      601 ggtcgaggcg acttcgtcgg cgatccacag cgtatcgttc ttcagcgaga gcaggtaatc
      661 agcgcccgcg cgaccgccgc gcagcagctc gtcctcgtcc atcgagtcca cgctgacccg
      721 atgcccggcg gcatgcagcg cggcgacgga ctcttccaga tgcgggaacg gcgtcttcgg
      781 caggcagccc agatcgatca cgtccgcacc ggaatcgcga caggcacgag cctgctcgag
      841 aatcacctcg atgctcatgt tcggcgcgtc cacgatttcg gcgaagatgc gcacgtcgta
      901 cttcgaaagg tccagcggct tgccttcgcg gccgaagaag cgcggcaggt ccttgaggtc
      961 ttcgggcccg cgctggaaag gcacgccgaa atgtgcgctc aagggttcaa ggtcgccgcg
     1021 gcagcggccc ggcacgatca cccggttcac gccaagcgca tccggcagcc tgcgccggat
     1081 catgtccgag gtcatcagtc ccgccacctg gatggacagg acgtgcacgg cgtgcgagaa
     1141 gggcgccggc tgcatcgact ccagcacccg gtgcaggctt ttctcggcga gccggccggt
     1201 caggaacagg aggtgttcag gcaatccggc ctctttccac tctctttttc agagcgatcc
     1261 gaagttcgtc gatcgatatc accactgtgg tgtgttcgaa cttccctagt ttgcccacgt
     1321 tttccagatc gatcgggcgc ggatagaccc agacgtcacc gcccggggcc tcggagatga
     1381 gttcgggtgc cgtgtcgcag gcaaacacga tcgaagcaat gcggcacttt cccgcctgcg
     1441 cgtaaatgtt ggtcgcgagc gtgtcggaaa tgccggccac gcacttggcc acggtgttcg
     1501 aggtcgcggg tgcgatgacg acgctatggt aattgccttc gtacagaaag gtcaccggca
     1561 ctgcgctcgc ggtcgtgtcg cggtagatgt ggatgtcctc cttgagtttc ttcagatcgt
     1621 agccatacat tttcagaact tccgctgccg ccttggtcag aaacaggtcg acgttggaca
     1681 gcgtgcgcac gatgtccagg cactccttga ggtaatggcc ggagccggtg agtgcccagg
     1741 cgatgcgtgg ctccttgact ttagccataa cagtgaatgc tacgcgcgct gcgttgagcg
     1801 cggttgcggg agggaaaagg attcatcgga tcgaagatcc tctggttggg tcccgcgcgg
     1861 agagctgttc accgcatctg ttcaataatg gaacgcccgc gcgaccggaa cgcatggcgc
     1921 cgatgttagc cgtgttcggt gggaaacgga acacgctggc atcgctgcga tagttggata
     1981 gaatggcccg gagcgaaaac cgtagcgaat ttgtgagagt gtctgtggac gcaggaggat
     2041 gagatggtgg aaccagccga gaaagcctac agcgaggccg aggtcaagca gcgcctggcc
     2101 gtggaactgc cgcattggta ttacgaagac ggctggatca gaaggaagtt caagaccggc
     2161 ggctggaaag gcacgctcat ggtggtcaac accgtcggcc acctcgcgga agctgcgtgg
     2221 caccatcctg acctcaccgt ctcgtacgcg ttcgtgatcg tgaagctcgt cacccattcg
     2281 gcaaagggca tcaccgacaa agacctcgcg ctggcgaaaa aaatcgagga agtgattcag
     2341 tggcaaccgg gcaaggaagg tggcgtgctg gaaggtacgc cgaacgatga ccagcgcttc
     2401 aagtacatca agtatgactg agggcgtagt ccggccatcc gtgcgggcag acaggaattc
     2461 atccggaacc atgacaggcg ccgctccgcc catggacatc atcttcgttc gcgacatccg
     2521 gctcgaaacg ctgatcggcg ccttcgagca cgagcgccgc cagccgagaa ccttgcaggt
     2581 caacgtcgaa atcggccgcc cggcgattcg cgcgtgcaag actgaccaac tcgccgatac
     2641 gatcgactat gcggccgtgg tgagcgtggt ccaggaattg tttgcttcgc atcgcttcca
     2701 cctgcttgaa ccgctggccg acacacttgc ggacctgatg ctctctagat tcgaagcgca
     2761 gtgggtgcgg gtcgagattt ccaagatcgg tgtcgtgccc aacgcccgcg acgtcggcgt
     2821 gcgcatcgag cgtagtcgct gaataagaca agaaccaaat caagagcgtt gacgcaaagg
     2881 gcgcaaaggg aaagaaagtt acgcaaagga atgcgggact ggatgcggga gcggtttgcg
     2941 ttacttggca tggttttcct tcgcgtacct ttgcgaccat tgcgttgaac gcgtttcggg
     3001 tttgaacctt tctgtgaccc atgtgagagg aggggaaccg ataggctgga aacggatatt
     3061 gtgctgcgca atggattctc ccgatgcgag gctgtaggga aggcgacgca gcgtcgatgt
     3121 gtggctgtcg aaggtttccg tttctcagga gtaaatcaag aaattcattc gcttgcggat
     3181 gcactgcggg ctccacggaa gattgcctgg agcccaaatc ggcattgtga agaaatcgtg
     3241 cagtgcaaaa tgaatttttc ctcctgcgta ccggcagggc tggcacgttg accctgtcta
     3301 gggtctgtgc ttgactgctt gcaagcgccc gctggaatgc cggtttcctt acccgagatt
     3361 cccggttccg gtgaatagtc aaaaagggtt catcaggacc cctgggcata agcgctcttg
     3421 tccgactgaa tctcaagaca agtccggaca ggggaggagg tcggtatgaa tcacaaccag
     3481 atcaacaccg acgtcacggc aatccgccgt gcatacggtc aattcctcga gaagggggat
     3541 gttcccgagg gtgttgtccc ggatttcatc aagcggtcgt ggcaacgcag cgtggctttc
     3601 ggcgtgccct acgatcgcat ccgcgaagtc gggcgcatcg atgaaacgga tctgcgggtg
     3661 gtggtgagcc agtacggcag cctgatcaat ctcgcggctc ccgtgatgga aaatctccac
     3721 caccagctca ccggatcggg cagcgtagtg ctcctgtgca gcggcgaggg cctgatcctg
     3781 cattcgatgg gcgatccgga tttcattccc cgggcccagc atgtcgcgct caagcctggt
     3841 gtttcctggg ctgaggagca gaagggcacc aacgccatcg gtaccgcgat cgtcgaacgc
     3901 gaaccggtgg tgatctacgc cgggcaccac ttcatccggc agaatcattt cctggcctgt
     3961 tccgcctcgc cggtgctcga tccgaacggc gatgtggccg gcgtgctcga catcacctgc
     4021 gactaccgcg gccaccagcg ccataccatc gcgctggtac agatggcggt gcgggtgatc
     4081 gagaggcaga tgttccaaca tcgcttttgc cgcgacgtgg ttttcaacgt acaccctgag
     4141 cggaactatc tcggcagccc gttcgacgtg caggtagctt tttcgcctca tggcgattac
     4201 ctggcggcaa cgccggcaac ctgcgggcag ctcgacctgc ggccgggtgc ctttcgcggt
     4261 ctgttcgacg agctgttcga cgcgtcttac gggcaggtgc tgcgcaaact cggatcggtc
     4321 tcgccgccgg tgatacaggt gcgcctgcgg cacaacggca tgccggtgta tctgcaattg
     4381 gcgcagccca cggtggtcgc gaaaacaggt catggtacgc ccgtggattc cccggcgccg
     4441 aaacggcaac cttcgcccga cggccagcgt acgctgcacg aactgaggct cgataccctt
     4501 ggcgcatccg atccggtgac gttgttcaac atcgaaaagg cgcgccgcac gctcggccgc
     4561 gacattccga tcctgatcga aggcgaaacc ggggtcggca aagagtggct cgcgcgcgcg
     4621 atccacaata gcggcccgcg cgccggcggc gagttcgtgg cgctgaactg cgcctccata
     4681 tcggaaggtc cgctcgaggc ggaactgttc ggctatgagg agggcgcgtt cggcgccaag
     4741 cacaaggcag cgaccagcaa gatccagcag gccgacggcg gcacgctttt cctggacgag
     4801 gtcggggaca tgccgctgaa cctgcaggcg cggttgctgc gcgtcctcca ggagcgtgcc
     4861 atcacgccgg tcgacggcaa gccggtgaac gtcgacctag cggtcatctg cgccactcac
     4921 cacaggctgc acgacttggt gcgggcgggc ggctttcgcg aggacctgta ctaccgtctc
     4981 aacgggctca cgctgttcct gccgccgttg cgggaacgca aggacgtgct tgagctggcg
     5041 acggcgctgg ccgcgaaggg atgcgttgcc ccgcgctgca tcgagttctc cgacgaggtc
     5101 atggacatct tcgcgcatca tccctggccg ggaaacctgc ggcagatgca ttcggtgatc
     5161 cgcaccgcgc tggcgatgat ggacgcgggc gagaaactcg gacgccggca tcttccggaa
     5221 gattttctcg aagagctgac gctcgcgccc gccgctgcag ccgcgcgcga agcgcttgct
     5281 gcgcaagcgg tcatgtcgtc gatgtacgcg gacagtctgg aacagatcga actgcaggca
     5341 atccaggaaa cgatccgcca atgcaaaggc aacatctccg ccgcggcacg ccagctcaac
     5401 gtgagccgca ctacgctcta ccgcaagctc aagaactcct ccttcgcgac gcctgatccc
     5461 gaccggcgcg gcgccgctcg caagcgcggc gacgacctgc acggttgacg cgcctgcgcg
     5521 cgcaattcat ccggacatcg cgaagccgcg cgtgaaatgc agcgacgggc acgccggaga
     5581 tgctgatgca acctgcaaca gttgtccaga actgaaacac tcctcgaaga ttgccgtcgt
     5641 ttcaccttcg agcgcattcg atttccattc aggaaaagtg ttgaccctat aaaagcactg
     5701 tgcttgtata gggatacacg ttcggtgaga tttccgtccg gaaatgcatg tgccggaaat
     5761 gtgtgtgcca ttgcggtgaa gaagggtgat acgcaggcac cgtgaggcaa acaaaacgct
     5821 ctgtggtcaa cttcaaacac tataagcagg aggaggcatg agagtcactt tacgttatct
     5881 ggttttgccg ttggccctga ccgccgcagc tggcgtggca caggccaact cggacgttga
     5941 aagactcact gcaaatccga agaactgggc aatgcaggct ggcgacatgt acaaccagcg
     6001 ctacagcaag ctcggccaga tcaacgccgg caacgtcaac aaacttcagg tcgcctggac
     6061 tttttccacg ggcgtattgc gcggtcacga aggctctccg ctggtcatcg gcgacacgat
     6121 gtatctgcac tcgccattcc cgaacagggt cttcgcgatc gatctgaata ccaacaagat
     6181 caagtggaag tacaccccca agcaggatcc tgcggtcatt ccgcagatgt gctgtgacac
     6241 ggtgaatcgc ggtctcgcct acgcggaagg caaggtattc ctgcagcagg ccgacagcac
     6301 gctggtcgct ttcgatgcca actccggcaa ggtcttgtgg tcagtgaaga acggcgatcc
     6361 cagtgtgggt gcggtaaata ccaacgctcc gcacgtgttc aaggacaagg taatcaccgg
     6421 catcagcgga ggcgaatggg gcgtacgcgg ttacgtggcc gcttacaaca tcaacaccgg
     6481 cgacctggtc tggaagggct acagcaccgg accggacgac gagatgctga tcgatcccgg
     6541 gaccaccacc acctggatgg acggaagggt gcagccggtc ggagaggact cttcgctcaa
     6601 gacctggaag gaagacctgt ggaagatcgg cggcggcacc acctggggct ggtacagcta
     6661 cgataaggcg ctcaatctca tgtactacgg caccggcaac ccgagcacct ggaacccggc
     6721 gcagcgtccc ggcgacaaca agtggagcat gtcagtctgg gcccgcgacg tggataccgg
     6781 caaggtgaaa tgggtttatc agatgacgcc gtacgacgag tgggatttcg acggcatcaa
     6841 cgagatgatc ctcgccgaca ttccggtcaa gggcaagatg accaaggcgc tggtgcattt
     6901 cgaccgcaac ggcttcggct acacgctcga ccgtaccaac ggcgcgctgc tggtcgcaga
     6961 gaagtatgac ccgaaggtga actgggcaac ccatgtggac atgaagagcg gccgtccaca
     7021 ggtcgtttcc aagtactcga ccgcgcaaaa cggtccagac gtcaatacca agggagtctg
     7081 tcccgcggca ttggggtcca aggacgagca gccggcagcg tactcgccca agaccaggct
     7141 cttctacgtg cccaccaacc acgtgtgcat ggattacgag cccttcgcgg tcgagtacac
     7201 ggcgggccag ccgtatgtgg gtgcgacgct gtccatgtat cccccgcccg gcgaaaaaca
     7261 catgggcaac ttcatcgcct gggatgccgg caccggcaag atcgtctggt cgaagccgga
     7321 gaagttctcg gtgtggagtg gcgcacttgc cacggccggt gacgtgatct tctacggaac
     7381 cctggaaggc tacctcaagg cggtggacaa caagggcaac gagctgtgga agttcaagac
     7441 cccgtccggg atcatcggca acgtgttcac ctatgaacac aagggcaagc agtatgtcgg
     7501 cgtgttctcc ggcatcggcg gctgggccgg catcggcatg gcagccggct tggagaagga
     7561 taccgacggc ctgggtgcgg taggcggtta taaagaactg aaccagtaca ccgagctggg
     7621 cggctcgctc acggtgtttg ctctcggaaa ctgacaagcc aaaaaaagtt cgtatgcgaa
     7681 atggcatccc ggtgcgttta cgggatgcca tttctttatc accccgggga tttacccatc
     7741 atgagcactc caatgtcggg aaggaggatt ctggtcgccg tgttcgcggt gattgcaacg
     7801 ctgcaggcag gggccgatga atccgctgga agcaatctgt acaccgtggt agatggctac
     7861 aaggtggatc cggccacgat gaaaggattc cgcgcctggc gtgctgcggc ctgcgaccgc
     7921 tgtcacggcg ccaaccagga gggcctggtc gggccttcgc tgatcaacag cctgaaggtg
     7981 ctcagcaagg aggagttcgt caagacggtc accgagggca ggctcgacaa agggatggtc
     8041 tccttcaaga ccagcaagat cgtaatggac aacctggaca acctctatgc ctatctcaag
     8101 ggccgttccg acggcgccat cacccgcgcc aaggtcgagg aactgaaacc ttgacgctct
     8161 gcggcagtca taccggcgtg ttgcaggctt cgcaagcatc gaatgaccgg cgaggacagg
     8221 gcgcgattgc tttcctggcc gcgtgtgtgt tcgttgtggt cggggcgggg gcggcggcgg
     8281 acgaacccga cgaggccgaa cgcaaagtgt tcaaggtgtg cgccgacccc aacaatctgc
     8341 cgttttccaa tcagcagggc gaaggttacg aaaacaagct cgccgagctt ttcgggcgtg
     8401 aaatgggcct gccggtcgag acctactttc aggcgcagcg gctgaatttc gtgcgcaata
     8461 cactgcggtt caagctgccg ggggaagact accgctgcga catcatgatg ggcgtgccgg
     8521 tgggattcga gcaggtcgct accacgcggc cctattaccg ctcgacttac gtgctggttt
     8581 acatcaaggg ccgtggactg gacgcggtga acagcgttgc ggattttctg gcgttgccga
     8641 gagagcagtt gcggcgcctc aagatcggcg tgttcgaccg gtcgcctgcg agccaatggc
     8701 tgaagaatca tgacctggtc gatagcggcg tgccctaccg catgatgaat gcgcggccgg
     8761 attattatcc cggggagatc gtggaaaagg acctggtgca gggtaaggtg gacgtggcgg
     8821 tactgtgggg tccgatcgcc gggttcgcag ccacgcgggt caaggatcac gaggtggtgg
     8881 tggtgccgat gccgtccgaa ccgggggtga agttcgacta ccaggtggcg atgggcgtgc
     8941 gccacgggga aacgaaatgg aagcagcgca tcgatggcct gatcgaatcg aagttgccgc
     9001 agattcgcga aatcctgcag agttaccggg tcccgcttca ggacccgtga ggcgcgatca
     9061 cgaggcggca cggaattgcg ccgcgatcgg gcggaaaagc cggatctgta ttgaggcgaa
     9121 gacactgttt tcggctctga gccgggcgat gctgacgctg tacctgcttg cctgtgcaag
     9181 cgtgtgggcg gaccccggtc gtgcgcaggt gttgaaattg gcgccaagcc tggcgcgggt
     9241 tgaagccaac aagacgaccg gcgggtatgc atttggtacc gcggtcgcga tcgcgccgga
     9301 tcgcgtcgtc accaactgcc acgtcacgcg ggaagcgcag gcgatttacg tggtgaaggg
     9361 cggtttgcgc tgggatgcgc gcgcccaact cgccgatgtc gatcacgacc tgtgcctgct
     9421 atgggttccc gggctggatg ccagagcggc gccgcttgcc accagcgcga gtctgcacct
     9481 agggcagccg gtcgcggcga tcggttacgg cggcgcaacc ggaatccaga tctcgatcgg
     9541 cgacgtgatc agtctgcatg cgctcgacgg cggccaggtg atccggagca gtgcagcctt
     9601 ttccggtggt gccagcggcg gagggctgtt cgacgaggat ggcaggctgg ttggcattct
     9661 ctccttccgt cttcaggcaa ggggggccta cttcttctca gtgccggtcg aatggttcag
     9721 tgagcgcgtc cttgacctgt cccgttacca gcctgtggcg cctcttggcg aagcaagatc
     9781 gttctgggag cggccgcaag cagagcagcc ctatttcatg cgtgccactt cgctggaagc
     9841 tgcgcacgat tggcctgagt tgctgcgctt caccgatacc tgggtgaagg ccgagccggg
     9901 aaatccggat ccgcaatatt ttcgcgacaa ggcattgaac gcacccctaa acggaaacag
     9961 ggaactgcaa tgaaaatgcg cctgccagta ctgctgggat tcctcgggtt cgcggcgact
    10021 gcaccggctg ccgatgccgc cgggaacgat tttccgacgg tggcccgggt ggagtacgtg
    10081 caggaatgca tgcgcaacca tcccggcagc cgctacgaga tgctctacaa atgctcctgc
    10141 gcgctcgacc gcatcgccgc ggaaatccgc tacgacgagt acgtcgagct gtggacggcg
    10201 gcgaacgcgg tctcgatcgg cggcgagcgc ggcgcgcagt tgcgtgacgc gcagatgtcg
    10261 caggacatgg cgaagaagtt ccggtccatc gagggaaaag cgcagaaaga ttgctttctg
    10321 cgcaaggagg aatgacgttg ggcgcaatgc ggcgtggcat gcgcaagggc tgcggtccac
    10381 tattgctggg actgggcttg gcgctgtcca tgcctgcggc acagggcgcg gaggcggttc
    10441 ccgataccga tatctggctg aaggtgcgcg agagcttctt cggcgcgcga ccgatcaggg
    10501 ccgacgccga ccaggtgatc cggctggagg tgccttaccg ggcgcaggac gcggccaccg
    10561 ttccgatcgc gatccgtact cggattgcgc aggccggcga accctacatc cggaccgtct
    10621 atctcgtcgt ggacaggaat ccgtcgccga tcgccgcgat tttccgtttc acgccggaca
    10681 gcggtcgcgc ggaaatcgag acccgcatcc gcatcgagga gtacacgccg gtgagggcga
    10741 ttgccgaaat gagcgacggg gaactgcacg gctcggcgcg gttcgtcaag gcggcgggag
    10801 gctgttcggc accggcgggc aaggatcccg aggccgcgct ggcgcggctg ggccgcatga
    10861 agtttcatct tgatcagtcg gtggtgctgg gcgcgcccaa cctggtgcaa ctgatgatca
    10921 gccacccgaa tcactccggt ctggcgatgg accagttgac tcacctctac gtgccggcct
    10981 acttcgtcag gaccgtcaac gtcacctacg ccggcaagcc ggttctcggc gccgaagtgg
    11041 atttttcgat cagcgagaat ccgaacttcc gtttttactt cgtgccgagg gaagagggcg
    11101 aactgaaagc ggaagtggtg gacaccaaag acctcaggtt cgagtacgtg ctcgcggtcc
    11161 ggcgcggcct gtccaccaag gcgagcgaat gaagaaactc tgcttcttca ccgcagaggc
    11221 gcaaaggcgc agaggaaacg cagagaaaat cataaagggg aatgaattga gatcgctgct
    11281 cgttttcatt tggctcgggt agtttgtccg aatcacacgc atctaccgga accaggaccc
    11341 gccatccccc tgccgcgttt cgggcaatac aggcacgaac cgaatccatt ccatcctggt
    11401 tttcctttgc gtttcctctg cgcctttgcg cctctgcggt gaaggtctag agagaaacag
    11461 gccgggaaag atcctgaccg ggcagcaggg catcgatcag cttgcgcagt tgctccgcgg
    11521 ttgccggttg cagcgcggcg ccgggatggc gcaccttgct ggtcttgagc ccgcccctca
    11581 gccgtagaat ttccttcctg atggcgaccc ccggttgctg ctcgaacacg accagcggca
    11641 ggaagcgcgt atagatctcc tgcgctcttg cccagttgcc cgcgcgggcc gctttcacca
    11701 tcgccatcag cacctcggga aacgcgaagc cggtgttgaa accggccgag ccagcctgca
    11761 agtcaaacag tccgtacagc gcgccgagtc cggtcaggat cggcaccttg cgttccttca
    11821 tgccggcggc cagtgcgcgg attttaggtg cggtcggcac agcctcctcc ttgatgctgg
    11881 cgacctgcgg caattccctg atgatcctca gcagcagcgc cgccgtcata tgcacctgcg
    11941 tggaggccgg gtgatcctgc gccacgatcg gaatgcggat gccttccgcg atgctcctgt
    12001 agtactcgaa gatgcggtcg tcgttcggca cggtctcggc gtggggcgtg accatcacgg
    12061 ccatggcgcc gagattctcc gccatctgcg caagttccgt cgcggcgcgc gtgccgctgt
    12121 ggctggcgcc gacgatcacc ggcattgcgc ccgccgcggc aaccgcggtg cggatcagct
    12181 cggcgcgctc gctgtcgagc aggcggttgg cctcccccag cacgccgagg acggtgatgc
    12241 cgtccacgcc gagaccggcc atgaagcgca ccagccggtc gaaggattcg aggtccaggt
    12301 tttcattgtc gtcgaagggc gtcgcaagga tcgggtacac gccttcgaag gtaagctgcg
    12361 tggccattgt tcttatctcc gcgatggcgg cattctcagg cgcgcggggg ccctctgtaa
    12421 agggcgggcg cgctgcaaga gacggtacaa tcgcgcctct cacacgcaaa gaatccgggg
    12481 agggacttca tggatatgcc cgtcaatcag ctcaagcgcg cgttgcgcga gggacggccg
    12541 caagtcggat gctggctgag cctcgccagc cacgcgtcgg cggaaatctg cgccggcgcc
    12601 ggcttcgact gggtgctggt ggacaccgag cacgcaccgg cggacctgcc gcaggtgcac
    12661 caccagttgc acgcgatcgc ggcatatccg gtctccgcgc tggtgcggcc gtactggaac
    12721 gatacggtcg tgctcaagcg gctgctcgac atcggcgtgc aatcgttcgt cattccctac
    12781 gtgcagagtg ccgaggaggc gaagcgcgcg gtggcggcgg tgcgttaccc gccgaggggc
    12841 gtacgcggcg tgtcgaccaa ttcacgctcc aaccgcttcg gacgggtaaa ggattacttt
    12901 gcccacgccg acaacgagat ctgcctgatc gtgcaggtgg aaacccgggc ggcgctggcc
    12961 gagatcgaaa agacggcggc gatcgacggc atcgacggcc ttttcatcgg gccgcaggac
    13021 ctcgctgctg acttcggcca tctgggcaac ccgggccatc ccgaagtgca ggcggcaatg
    13081 gcggaggcca tcaaacgcat tcgcaaggcc ggcaaggcgc cggggatcct ggcctttgcc
    13141 gaagcagacg ccaagcgctg gctcgagctc ggcgcgctgt tcgtcgccgt aacctcggat
    13201 cagttcctgc ttgcccgcga aagcgcggcg gtcgccgctc gctttaaagg ctgaaaataa
    13261 cacggccttt tacaaacagt agttcttcac cgcagaggcg caaaggcgca gaggaaacgc
    13321 agagaatcac aatagggtat tgaatccaga tcggcactcg tatttacccg gatcgggtcg
    13381 cctctcaaaa tcacacgcat ctacgggaac cagaacccgc tatttctcta cccagaatcg
    13441 ggtaacgggg acccgaaccg aattaccagc ccatccttgt tttcctttgc gtttcctctg
    13501 cgcctttgcg cctctgcggt gaaaaggtct tccgcattac cgaaggtgct gtttcttctc
    13561 ctcgatgctg tccaccatgt aacggatgcc gcgcagccat gagttgtcgg tgttgctgcg
    13621 caggtccacc gatttcacgt acagcatcct gccagagctg acttccttga cctcgatgtt
    13681 gatattgagg atcaggttgc tcactttctg cacccagccg atgatgatga catcggcgct
    13741 gagcgctctg ccgatgtcga tctcgcagcc gttgcattcg cgcagttccc cttgcgcctt
    13801 cagtccggag atcaggcttt cggccgcgct gttgtccgca atccggtaca ttccgcgttg
    13861 aagcaattcc cggcgcaagg catcgctgat caggccgatc ctccggttct gcgcatccct
    13921 ggtctcggga cgttcgtagt cccgcatgtc gtcgatcagc tcgaactcca tgactgccgc
    13981 aattctgggc gccggcgcat cggtctccga cgcagctaac ggcgtggcca ggcagaacaa
    14041 caccgaaaat gcaacgtatc gatcgattgc actcatcgac gagaacccgc agccggcatg
    14101 cgtgcgccat tgagcgcggc ctccaccgtg tcggcaatcg cctcgggagc agcgccgacg
    14161 tcgacagtga tcgcgtcaat gggttcttcg agcgcggcaa actgcgactc gagcagcccc
    14221 gccttcatgt aatgcccctt gcgggtcttc agcctccact cgataagttc gaagctgcct
    14281 ttaaggtgca cccagcgcaa gttgctgcag ccggcagcga gccggtcccg atatttctgc
    14341 ttgagcgccg aacaggccag cagcacgttc tggccattcg agtccttttc tttaaggagg
    14401 ccattgagcc ggtccaacca cggccagcga tcctcgtcgg tgagcgctat gccggagcgc
    14461 atcttttcga tattggccgg cgggtgaaaa tcgtccgcgt cgagcagcgg ccagccgagc
    14521 cgttgggaaa gcagttctcc aatcgtggtc ttgccggcgc ccgagacgcc caataacacc
    14581 acgatcattt gcttttcgcc gcatgcacgg gatggccgcc gaaactggca cgcatcatcg
    14641 ccagcagctt cgaggcgtaa tcgtcctgcc cctggctggc aaagcgggcc atcagtgcca
    14701 ggctcatcac cggggcgggt acccccagtt ccaccgcttc gatcgcggtc cagcggcctt
    14761 ccccggaatc ggcgaccagc ggcttgatcc tttccagaga acgctcgttc ctgaggaagt
    14821 cggcaatcag gtccagcagc caggagcgca ccacgctgcc gtgacgccac atctcggtga
    14881 tcgcggcgag atccaggccg aattcggatt tggccttgag caccgacagc ccttcggcat
    14941 aggcctgcat cattccgtac tcgatgccgt tgtggatcat ctttacgtag tgacccgagc
    15001 ccgccggtcc gcagtgcgtc cacccgtgat cgggcgccgg cgccagcagg cgcgcgaacg
    15061 gctcgacgcg cgtgaacgcc tccttggcgc cacccagcat cagcgcatag ccattgtcga
    15121 gtccccagac gccgccggag actccggcgt cgacgaagcc gatgtcgcgc tcggcaagcc
    15181 acctggctcg ccgctgcgaa tccttgtaga gcgcattgcc gccgtccacc agcacatcgc
    15241 ctttggcgag cagcagagca agttgttcca gtgcccgctc tgtgacttct cccgccggca
    15301 gcattaccca caccacccgc ggcgccggca gggccgcgat cacgttcttc agttcggggt
    15361 gcgccgtgat gccgcgctcg tgttcgactg cgctgcgtgc cacggggctg acgtcgtgcg
    15421 ccgccacccg cacacctttg cgcgccagcc gtcgcgccat gttggcgccc atgcgcccaa
    15481 gacccacgat gccgatatcc atttgtgtcc cccggtccat ttatgcggcc attctccggg
    15541 gcacggggtt ccggcgcaat gcgtacccgt aacgaatccg gtaatctgcg cctctcggga
    15601 acaggagagg atcaagatgg cgcgagcggc attccggaat ctggaagagc tggcggcaaa
    15661 ggtcgggcag gaggtcgtag tgagcgactg gctcgaagtc acgcaggaac gcatcgacca
    15721 atttgccgac gccaccggcg accaccagtg gatccacgtc gacgtcgagc gcgcacgccg
    15781 cgagtcgccg ttcggcacaa ccattgcgca cggattcctg accctgtcgt tgctaagtca
    15841 tttcctcaac aattcgctgg agttcggtaa ttcgaaaatg ggcgtgaact atggctgcaa
    15901 ccggctgcgc ttcacggcac cggtgaaagc aggatcgcgg ctgcgcgccc ggttcaaact
    15961 gaaggagttt cagcggatag aaggcggcgt gcagatgatc tgggacgtgg cgatggaatg
    16021 cgaaggccag caaaaaccgg tgctcgtcgc ggaatgggtc gggcggcggt atggctgagc
    16081 gcttgcccgc gatttttgta tcccacggcg cgccgctgct ggcgatccag ccgggcccgg
    16141 cccgcgagtt cctttccgga ctgggcagga cgctcggcaa gcccaaggcg attctaacgg
    16201 tgtccgctca ctgggaaagc ggcgacgcgg cggtgagcgc cgtcgcgcga ccagagacaa
    16261 ttcacgattt ctacggattt cccgatgagc tctatcgaat cgagtacccc gcccccggcg
    16321 cacccgagct tgcggtgcag gcgaaggcat tgctcggcga gaaggggatc ggcgcacgct
    16381 tgcataccac ccgcgggctg gaccacggcg cctgggtgcc gctgatgctg atgtatccgg
    16441 acgccgacat tccggccgcg caacttaccg tacagaccgc gctgggcccg gcgcatcact
    16501 tcaggctagg cgaagcgctg cgcggcctgc gcgaccaggg cgtgctgatt ctcggctccg
    16561 gcggcgccac gcacaatctg cgtgagttcg gccgccatcc cgaaggcgcc gcgccgccgc
    16621 agtgggtgac gggattccag gagtggctgg cgcagaccat cgagggcgga aaatacgacg
    16681 atctagtgcg ttatcgcagt ctggcgccgg aggccgcgcg caatcatccg accgaggaac
    16741 atttcctgcc gctgttcgtg cccgcggggg cgggttcgcc cggtgtggcg ggcaagcgca
    16801 ttcaccgcag ccacacgttc ggcatcctgg cgatggatgc gtatcgcttc gactgacatc
    16861 cggccgcata aacgacgatg gcaagaacag acttcagatt cgcgcaccgt atgcgggtgc
    16921 gctgggccga ggtggacaag cagggcatcg ttttcaacgg ccattacctg atgtacttcg
    16981 acgtggcgat caccgagtat taccgcgcgc tgggctaccc ctatcccgag gggctggcgc
    17041 ggcacggaac cgatctctac gtaaagaagg tcgaggtcga gtaccaggca tcggccggct
    17101 acgatgacga gatcgacgtc tgcgtgcggg tcgcgcggtt ggggcgatcg agcttcgagt
    17161 tcctgcttga gatctatcgc gcggatcagc ttctggtcgg cggcagggtg gtctacgtca
    17221 atgccgaccc ggaaacccgc aaatcggcgg cactgccgga atttcttcgc agcggcatca
    17281 aggccttcga gcacacggca ccgaccgagg cggttgcgaa tccggcttaa aaaagcgggg
    17341 ccggggtgtc atacttcatg caattacgat ttctggcgcc ttccctgaaa tagtccgtcg
    17401 cgcgacaggg agattccggc tgccttccct tgaaaatacc acccgcataa actaaggaca
    17461 aaagacatgg atttcaattt ctccgacagg gtcagggatc tgcagaaaaa agtcggcgcc
    17521 ttcatgggcg cacacgtcta tcccaacgag caccgcttct tcgaggaaat cgaggaaaac
    17581 cgacgcaagg gcaacgcctg gatacccacg cgcatcatcg aggatctcaa gcccaaggca
    17641 cgtgccgccg gactttggaa tctatggcgg cccaaggacc acggcggcgc gctcaccaac
    17701 ctggagtacg cgccgctgtg cgagatcatg ggccggctgg cgtgggcgcc cgaggtattc
    17761 aattgctcgg cgcccgatac cggcaacatg gaagtgctgc tgcgttacgg cacgccggaa
    17821 cagcagaagc agtggctcac gccactgctc gaaggacaga tgcgttcggc cttcctgatg
    17881 accgagccgg aggtggcctc atcggacgcg accaacatcc agtgccgaat cgagcgccaa
    17941 ggcaacgaat acgtgatcaa cgggcgcaag tggtggtctt cgggcgcggg cgatccgcgc
    18001 tgcaaaatct acatcctcat gggaaagacc gaccccggca acgccgaccg tcacaagcag
    18061 cagtcgatga tcctggtgcc ggccgacgcc aaaggagtga aagtggtgcg agcgctgagc
    18121 gtgttcggtt acgacgatgc gccgcacggc cacatggacg tcaccctgga gaacgtgcgc
    18181 gtcccgacga cgaacctgct gctgggtgag ggccgcggct tcgagatcgc ccaagggcgg
    18241 ctcggacctg ggcgcatcca ccactgcatg cgcatcatcg gccaagccga gcgcgcgctc
    18301 gagttcatgt gcaagcggtt gaagtcgcgg gttgcgtttg gcaagccggt ggccgagcaa
    18361 agcgtctggc acgagcgcat cgccgaatcg cgaatcatga tcgagcaggc acgcctgctt
    18421 accctcaaag ctgcgttcat gatggatacg gtgggcaaca aggaggcgaa ggcggaaatc
    18481 gccatgatca aggtgatcgc gccgaacatg gcgcagcaga tcatcgactg ggcgatccag
    18541 gcacatggcg gcggcggcgt gtgccaggat tttccgctgt cctggatgta cgcgtggaat
    18601 cgcacgctgc ggctggcgga tgggccggat gaagtgcatc gcagccaggt tgcacgcctg
    18661 gaactgaaga agcacgctta acggccaaga acaaaaacag cgggacacac ccacagcggc
    18721 ctgatcgcaa tgatcgggca cgatgcgcct cgacggggaa atacttcaga aggcaccggt
    18781 cggttttccg tgtgcggccg ttcattgccg gccacgattt cagttacgcg gattgcgcgg
    18841 cctatgcgcg cctgacgcag catggtcatg gtgcagtgca cgcgcgagtc tcttgccccg
    18901 gaattcgacg actgcagtct gatcgctcca cggtcacaat ttcacgatac ggttattgca
    18961 acgcaacatc gcagctctgc cgcgacctca acacatgttc cgaataggct gaattttcgc
    19021 aacaaagccc gtcgcccgaa aaaccgcgtg gggagcggct ttgaggccat cggcaattta
    19081 cgttgcaatg tctttccgcc acgcgaacaa tgcgccgtaa agagttgaaa gacattttct
    19141 attcgtgtat tttgcccgag taaccaatag tagttgttac gcctctaagt ttcattcctc
    19201 actactgctc aagggggagt tttgcaatga agatctcgtt taacgttaat ggaaaacaat
    19261 cgagcgtaga cgtcccgcca gatacccctc tgctgtgggt gctccgcgac accttgggcc
    19321 tcaccggcac caagtttggt tgcggcaagg cgctctgcgg agcctgtacc gtgcatctga
    19381 acggaatggc agtgcgctcc tgtcaaaccc cggtcggttc cgtatccggc cagaaggtca
    19441 ccaccatcga aggactgggc gggaaccaca aggttcagaa ggcatggatt gcgctggatg
    19501 tcccgcagtg cggctattgc cagcctggcc aaatgatgtc ggcggccgcg ctgctcgcag
    19561 ccaacaagaa acccaccgat gatgatatcg atgccttcat gtccggcaac atttgccgct
    19621 gtggcacgta tccgcggatt cgcgcagcga tcaaagaagc tgcgaagatg gcctaaggag
    19681 gtgaaacatg gaaaacatca tcaaaaacga aacgcgtcgt gacttcctga aggtcagcgg
    19741 caccgtcgcc ggaggattgg cactcggttt ctatctgccc accgggccgc gcattgcgca
    19801 agcggccgtg caaaccaatt caccgaatgc ctggatcaag atcggcaccg acaacagcgt
    19861 caccatcatg tgcgcccggt ccgaaatggg tcaggacgta tatacctcga tgacgatgtt
    19921 ggtggtcgaa gaactcgaag tcgacatcaa caaggtcaag gtggagtttg ctccgcctgc
    19981 cgaggtgtat atcaatgccc ttctgggtgg tcagctcacc ggcggttcga ctgcggtgcg
    20041 cgacgcctgg gagaaactgc gcaaggcggg agcttctgcc cgcatgatgc tggttgccgc
    20101 tgcagccagc gaatggggcg ttgacgcttc tcaactgaag gcggccaacg gcatggtcac
    20161 cggccccggt ggcaagcagg ccacctacgg taagctcgcc gccaaggcgg cgacgatgga
    20221 agtgccgaag gacgtcccgc tcaagccgtc gagccagttc aaggtggtcg gcaacgtcag
    20281 gcagaaacgc ctcgatacgg ctgccaaagt gaacggtacc gcgcaattcg gtatcgacac
    20341 ccgcgtgccc ggcatgcttt acgccgcggt tgcgatggcg ccgatgatcg gcggcaaggt
    20401 cgccagctac gacgattcgc gcgccaagtc ggtgcccgga gtcaaggcag tggtgcaata
    20461 cagccgcggt gtcgcagtgg tggcggactc ttactggcag gccaagaagg gtaaggatct
    20521 gctgttgatc cagtgggatg ccggcccgaa tgccgacaac gatcagaaga agatctggga
    20581 aggcatccgt aatgcgtcac agcagcccgg tgcggtgttc cgtgagcacg gcgatgtggc
    20641 cgcgggcatg ggtcaggcgg cgaagactgt cgaggccacg taccagttgc cgttcctgtc
    20701 gcactcgccc atggagccgc agaacaccac ggctgacgtg cgcgccgaca aggcagtgat
    20761 catcaccccg acccagttcc agcaactcat cccgcacgtt gtcgcgggcg ccacgggtct
    20821 gaagcccgag caggtcgaag tgcataccac tttcctgggt ggcggtttcg gtcgccgcgt
    20881 ggaagtggac tatgccatcg acgcagcgga aatctccaaa gcagtcggtg cgccggtgaa
    20941 gatggtatgg agccgcgaag acgacatgac gcacgattcc taccgcccgg caggtctgta
    21001 caagctgacg gccggcctgg attcaggcgg caagccggtg gcgatgcgtt tccattccac
    21061 cagcccgtcg atctcggcac gtctgttccc gagcgtcgtg aaggacggca tcgacccgtt
    21121 tgcggtggaa ggcatcgaca attacccgta cgaaacgccc aacctcaagt tcacctacca
    21181 gatgcacgat actggcgtga ccccgggtta ctggcgcgcg gtgtcgcaca acctgaacgc
    21241 ggtggccctc gagtgcttca tcgacgagat ggcgcaagct gcgggcaagg acccgatcca
    21301 gtaccgcatc gacatgctcg acatgggctc gaccaagcat cagtggtccg gcctgtccgc
    21361 aggcgtgccg gtcggtaccc gcatgaagcg cgtcctcgag gaagttcgcg ccaagtccgg
    21421 ctggagcaag aagatgccgg agggcaaggg catgggcgtg gcagtgatgg aaggctataa
    21481 caccgtgatc gcaatggtcg cggaagtgac cgtgtcttca agctacgacg tgtccgtgga
    21541 caagatcacc gcggtgatcg atgccggtac cctggttcac ccggatcagg cgctggccca
    21601 gatgcagtcg accatcaact tcggtcagtc ggcatgtatg tggggcgaga tcaccatcaa
    21661 gaacggtggt gtcgagcaga ccaacttcga catgtaccgc gttgcgcgga tgaatgagaa
    21721 tccgaaggtt ctggatattc acttcatcaa gagcgacagc gtgcccggtg gtctgggcga
    21781 gcccggaacc gcggtggtgc agccggccat cggcaatgcg atctttgccg cttgcggcaa
    21841 gcgctgccgt accctgccgt tcaccccgga aaacatcggc ggcgcgtaac agcagagtcg
    21901 ttgcccggcc ttagaccggg caggagaaaa ggctccatga ccccccaacg ttttgcgttg
    21961 gggggttttt tattggctat cccggggccg gctggtttag aatcgttatt cccgttcgag
    22021 cagaacggta gcaatagagg aaagttatgg acagcgtgga cttgcaggtt ctgcgaagca
    22081 gcgtgacctg gctgagccag ggccatcgcg tgatccttgc cactatcgtg gagacctggg
    22141 gctcttcgcc gcggccggct ggcgcgatgc tcgcggtccg tggtgacggc ttggtggccg
    22201 gttcggtttc gggcggttgc gtagaagatg atctgatctt caggattcgc aacaagtcgc
    22261 tggcggtgga gaggccggaa atcgccacct acggaatcag caaggacgag gcaacgcgct
    22321 acggccttcc ctgcggcggc aagctggaat tggtactgga gccgatcagg gacaacgaca
    22381 gcgtcatcga cctgctcaat cgcgtcgatc gccacgagct ggtggcgcga accctggatc
    22441 tcgataccgg cagggtcgcg ctgcggccgg cgagccgcgc tgatgctccg ctcagtttcg
    22501 accgcaagcg gctgaccacc gtgcacgggc cgcgctggcg gctgctgatc atcggggccg
    22561 gtcagttgtc gcaatatctg gcgcagatgg cgcaggcgct cgactacagc gtcacagtat
    22621 gcgatccgcg cgaagagtat gccgatacct ggaacgtgga gggcgcggtc ctggagcgcg
    22681 ggatgccgga tgacgttgtc ttggcgatgg gcctggattc ccacagcgca gtggtggcgc
    22741 tgacccacga cccgaagctg gatgatctgg cgttgatgga ggcgctcaag tcaccggcgt
    22801 tctacgtcgg cgccttgggc tcgcgcctga attcgcagaa gcgccgcgag cggctcacga
    22861 tgttcgacct ctcgcaggga gagcttgacc gccttcacgg gccgatcggg ctgagtatcg
    22921 gcagcaagac tccgccggaa atcgcggtgt ccattcttgc ggagatgacc gctgtgcgtc
    22981 acggtgtgga cccgaatgga gtcggcgcca ggaaaagcgg ggaagttgcg gccgacggca
    23041 atcagcaggt atgcgcgacc gtttcgcggg cctgacccgc cggagcggat cataatgcga
    23101 tgggagcctg tgaggctccc atttttttta tcgagtttcg gggcgtgaca agggagggga
    23161 gaggctgatg aggttgaaga acaagatcgc aatcgttacc ggcgcgggct cgggtttcgg
    23221 cgaaggcatc gccaagcgct tcgcggagga aggctgcaag atcgtggtga acgacatcaa
    23281 cgtcgagggt ggcgagcgcg tggcgggcga aatcaatgcc gccggcggca aggcggcgtt
    23341 cttcaaaggc gacgtgtcga gggatgcgga ctggaagtcg atgctgaagg tcgcgctgga
    23401 taccttttcc ggattgaaca tcgtggtcaa caatgccggt accacgcacc gcaaccaacc
    23461 cctgctcaac gttgccgaag agcagttcga ccgcgtctac gcggtgaacg tcaagagcct
    23521 gttccacagt gcgcgcaaca tcgtgccgca cttccgccag aagggcggtg gcgtgttcat
    23581 taccgtggcg tccaccgccg gcgtgcgccc gcgcccgggg ctggtctggt acaacagcag
    23641 caagggcgcc gcgattgtcg ccagccgctc gatggcggtt gaacttgcgc ccgacaagat
    23701 ccgggtgaac gtggtcaatc cggtggcggg cgacacgccg ctgttagtcg agttcatggg
    23761 agccgacacg ccggaaatgc gcgcgaaatt cgttgcgacg attccccttg gacggttcag
    23821 caagccgctg gacgtcgcca acgcgtgcct gtatctcgca tcagacgagg cggagttcat
    23881 taccggggcg tgcctcgagg ttgacggcgg ccgctgcgtc tgaccttcac ttcagagtcg
    23941 acttctcacc gcagaggcgc aaaggcgcag aggaaacgca aagtaaatca agaaaaacat
    24001 gaatggggga aagaacataa actctgggtt tttcgttgat gaatggtccg tacacttcga
    24061 tgtccccttt tgcgccgatc agatttttgc ccagtcaagt tcttctttgc gtttcctctg
    24121 cgcctttgcg gttaatgctt cccctaagcg gtgaaggcct ggatgccggt ttgcgcccgg
    24181 ccgaggatca atgcgtggat gtcgtgcgtg ccctcgtagg tattcaccgc ctcgaggttc
    24241 atggcatggc ggatgacgtg gtattcatcg gcgacgccgt tgccgccgtg catgtcgcgc
    24301 gccagccgtg cgatgtcgag cgcttttccc gctgaattgc gcttgatcag ggagatcatt
    24361 tccggggctg cgcggccctc gtccatcagc cttccgacgc gtagcaccgt ctgcaacccc
    24421 agcgtaattt cggtctgcat gttggcgagt ttgagctgga tcaattgatt cgccgccagt
    24481 ggccggccga actgcttgcg gtccaacacg tagtcgcggg ctgcgtgcca gcagaattcg
    24541 gcggcgccaa gcgcgcccca ggatatgccg taacgcgcct tgttcagaca gccgaacggg
    24601 cccttcagcc cctcgacgtt gggaagcaga ttcccgtccg gaacgaagac atcgtccatc
    24661 acgatctctc cggtaaccga tacccgcagg ctgaacttgc cttctatctt cggagtgctc
    24721 agccccttca tcccgcgttc gacaatgaag ccgcggataa cgtcgcggtc gtccttggcc
    24781 caaaccacca tcacatcggc gaccggggag tgagtgatcc acatcttggc gcctttcaga
    24841 aggtatccac cgtcgacttt cctcgcccgg gaaatcatgc cgcccgcgtc ggagccatag
    24901 tccggctcgg tcagcccgaa ggcgccgatc aagtcgccct tcgccaggcc gggcagatac
    24961 ttctgccgct gtgcctcggt gccataggta tagatcgggt acatcaccag cgaggactgg
    25021 acgctcatcg aagagcggta gccgctgtcc acgcgctcca gttcacgggt gatgaggccg
    25081 taacacacgt gattgagacc tgcgcaaccg tagccttcta tcgtcgggcc gaaaaatccc
    25141 aggctgccca tttcggagag caccgcgcgg tcgaattttt cgtgacgatt ggcctccacc
    25201 acgcgcggca tcaatttttc ctggcaatag gcgcgcgccg aatcgcgcac cattcgctcc
    25261 tcgtcgctga gttgttcgtc gaaaaacaga ggatcatccc agcggaaaga ggggcgggtg
    25321 agaggagcgt tcatcgcggt ctcctggaga aatacgctga atggtgaagc aactccggac
    25381 gcgaccagcc cgccgggcgg tcacgcactc ggtacggcag gaagtataca cagcagtgcg
    25441 catgtgccgc gatggctgac gaaggtgaga cggcggggcc ggaaccccgg agccgggcta
    25501 ctttttctgt ttgccgcaga tgtcgaaacc atcgtgccag ccggtgacgt attggggctc
    25561 gcgcttgaaa cgctgctcgt ctttcttgtg gccgactgtg cgcttggcac tctcgcagcc
    25621 gtcgagatag ccggcacgga actcgggcgg gtacccgccc agattgacat tgggctcttt
    25681 cttctggacc gcgcacgcgt gcagcaaggc ggccgccagc agcaacgaca gtctcttcat
    25741 gaattcaccg gttccggccg tcgtccctgg tcttttcgac gagcgtcgcc aaagtgtttc
    25801 gggactgggc ggcaaactgc ccgccaaggc taactgcccg gcgcgcgact tcgagtgcct
    25861 gcgtgagctg tccctgcgca tccaatgtga gcgcaagatt gttcaatgcc ggcaccgggt
    25921 cggaggccgc atcggccagc ttcgtgaacg cagtttcggc ggcttgccaa tcctgaaggg
    25981 aaaacgcggt attgccaagc cccagcaacg ccagttgatt gtgcggccat ttttccgttg
    26041 cggcggaata gacatgccga gctgccgttg cggaagcgac tttctcgaac gcgacggcgg
    26101 cctgcacata atcgctctcc ccgacggttg cagggatggt tccaggcggc acagtcacca
    26161 tggcccagta gcggctgcgc gcccaggtgc gctcgaacgt cggcatcgcc atgacttgtc
    26221 gtctggttcg gcccgagcga agaatgatct ggtcggcggc gaggtcgtag ccgaccacaa
    26281 ctgcgtaatg ccacatcggt acccagtcca gggcaaggtt ctgcagcacg atgaccggtg
    26341 tgccgttggc tacttccgcg agcaacgcgt ccagtctggg cgaaagcgta actgccagcg
    26401 cgtcgtgacg gcgggctgcg gccagcatct ccacttgcag gctgccgcga cgggccggca
    26461 ggaaaacctg ccgcgcaagg tcctcgggca tcgcgcttat gccggcggcg ttgagtgtgg
    26521 tggcaagtgc ggccggtccg cactgaaatt cctcctgggg gaaaaacggt gactggtcca
    26581 tttcgacggc gccggacagc agcggcgggg gtgcactctg gagcgcgcgg ctttgcgggg
    26641 cggcacagcc ggcgagcagg gcgcagataa aaaaaacgcc cgataaggga ggaaatcggg
    26701 cgtcaaacac gcaaaagcgc atcgaaggag tttatttgat cgggtgggta aagctgaaga
    26761 ctttagtaaa tcctagaatg tcggttatga gaaggttgtt tcaaatcgac gcacgcttta
    26821 catcaaccca gcgggatgac cgtgccattg accaacttga cgcgctcacc gatgctaaac
    26881 ggcggacgtt gattttcgtg gatcgtgcgt accgtgccat tctccatgcg cactatgacg
    26941 acgaagctaa aggaactgag tttttcgctg tcgatctgat aggccctgcc ggttgtaccg
    27001 agtgccgcgg caagcttgcc gtcgccgtcg cttgcctgtt gcgctttgat ggagtccacg
    27061 acaccgcagt ttgggcagga aggcttacgt gcggggaggc gtgcgccgga gcggaagctt
    27121 accggtttgc cctgcgccgc ggtttccccg gcgctgcggg tcaacccctc gtggataaga
    27181 ctggaaggca cccggtccga accggacgtc ggtaccgcag gtgcgccgcc gaatgcggaa
    27241 aaggggttca ggctcgacct ggcaatcggc aggtgacccg ttattgcggc gattccgatg
    27301 ctgctgaacg cgactacgga agcggccgcg gcgatcgtaa ggaggggtaa gaggcggttt
    27361 ttctggtcca ttttctttta gttggtttct tttagttggg ttcttgtaat tgattgtttc
    27421 gattcgatcg gccagcggac gcggccccgt tcggagccgc cttccgcgca gccgttttct
    27481 taacccttta aagcaggctt catgccagaa aacattaagg taatcgcggc agatagttat
    27541 gcggcgccaa ggacttggca ccggccgagg ttgtcgccgg aaagcgacgc cgccgcccgg
    27601 atcgggccta actgcttaat caaaaatgga aattctatgt cgtccgggga cgacacgacc
    27661 ttgccggcta aagcgccttg aacagcttgg gatcaaggtg gtgcctttgc agcagtttgt
    27721 agaattcggt gcgattgcgc ttggccagtc gcgccgcctg ggtgacgtta ccgcccgtga
    27781 tctttaggag tttggcgaga tattcgcgtt cgaactcgct gcgcgcagcc tcgaaggatg
    27841 acagttcggc agtctgcccg cggatggcat tttccaccag tgccggcgga atgatttgcg
    27901 tggtggacaa cgcaaccgcc tgctcgacga cattgtacag ttcacgtacg ttgccgggcc
    27961 acgatgattt caccagtatt tccatcgcct cggaggcaaa tccgttgagc ggtttcttgt
    28021 acttgtcggc aagttgcgcg aggaagtacg cggcgagcac cggaatgtcc tcgcgccgct
    28081 ccgcgagcga gggcagcgcg aacgaaacga cgttgaggcg gtaatacagg tcttcgcgga
    28141 acgccccgat cttgattgcg tcctcgaggt tgcgatgagt tgcggagatg atgcgcacgt
    28201 ccacgggaat agtctgggtc gagccgattg cccgtatctc tttttcctgc agcacgcgca
    28261 gcagttttac ctgcagcggc agcggcatgt cgccgatctc gtcgaggaaa accgtgcctc
    28321 cttcggctga ctggaacagc cccttgtaat cccgggcggc accggtgaac gagcctttca
    28381 tgtggccgaa caactcggac tccaacagtt gttcgggtat ggcgccgcag ttgacggcaa
    28441 caaatggctt ctcggcgcgc gggcttgccc ggtgcaccgc ccgtgccagc agttccttgc
    28501 cggtgccgct gtcgccgcgg atcagcacgc tggcatcgct ggcagccact agcttggcct
    28561 tgccgagaat gctctccatc gccgggctgc gggttatgat ctcgacgcgc cagtcggtgt
    28621 taccggtcgc cttttcggca tggttgccgc tcagggtgat ggccttttcg gcctgcagca
    28681 acagttcctt gctgtcgaaa ggcttggtca gatatccgaa aacaccgcgc tgagtcgccg
    28741 caaccgcatc cggtatcgtg ccatgcgcgg tgaggataat gacgggcagc gccgggttgc
    28801 tcttgtggat gttctcgaac agcgccatgc cgtccattcc gctcatgcgc agatcggtta
    28861 tcaccagttg cggggtggac gcggatagtc gcgccaacgc gcgctccgcg ctgtccgcgc
    28921 tctccacggc gtagcctgcg ccttccaggc ggaatgtcag aaggcgcaga agatcggggt
    28981 cgtcgtcgac gatgagtatt cgtgcttttg acatgtctac cttctctttg gtggcgtcgt
    29041 ctgctcgcgc tcgatcatct tcatttccat gtcgagaata gcctccagtt tctgctgcag
    29101 ggcgtccgcc cggcgttgtt cttccttggc ttttgccgtt tgctgctgag ccgcctcatc
    29161 caggcgcttg agttctgaaa gctgtgtatg gagcatgagg gccagcgcac gcagcacgga
    29221 gttttcatag ttcttatcgt tcacaaaggg ctgaaccagg ttcaatgctg tttggtcatc
    29281 gcgaaacccc gtgccgggca gcgacagcag taatgcgagc cgtacccggt tgagttcgga
    29341 ctggtcttta tcgaattcct gacgagtcgt attgagctcg cgcgcaagtt ctgcaccttt
    29401 gagcatgagg acattttcgt aaaagcgcag cgctcgggcg tcattgcctt gcatgggctc
    29461 gaccaccacc tggtaccacg ggtcgcgacg cgtgtttggt ccggtggttg ccgcgcatcc
    29521 gccaataagt gtagccagga caacgaacga cactatgaac agcctgatca cgcggctgcc
    29581 tccatctgcc gggtcggcaa aatcactcgc aggtgtgcgc cggggctgcc gtcgtcgatg
    29641 atttctgcgc tgccctgatg aaccgtcgca tattcgcgca cgatcgccag cccaagcccc
    29701 gtgcctttca cgggcccggt gtacacggtg ttgccctggt agaagggttc gaaaacgcgt
    29761 ccgcgttctt cgggagatat accgggaccc tcgtccatga cgtcaatcac cgcatcggcg
    29821 ccgcgtcggc gcagctgcac gcgaatagtg ccgccaacgg gcgagaattt gatggcattg
    29881 gacagcaggt tgtctatgat cgcttctatc ttgttctcgt cggcttcaat ctggaggtcc
    29941 gaggcggaca ggttcaagct cagattcttg cttcgcatgg tcaatgaatg ctgttgcgca
    30001 acgcgcgtca ccatggtttt cagatcgaat tgcgagaacg tcagtgccga agtatgaaac
    30061 tgtacgctgt gatagtttaa caagtcctcg atcagcttct gcagccgcaa cgtgttttgc
    30121 ctgaggatgc gggcgatctc gaactggcct gcggtcattt tcccggttac ctcatcggac
    30181 agcagttcca cgccttcccg cagcgccgtc aggggcgttt tcagttcgtg cgatacatgg
    30241 cgcagaaagc gagatttctg ttcttccaga tcgagcagac gcagccgcag ccagtcgagc
    30301 tggtgtccca gggattccag gtcggcaggg ccgttgacgc tgacgggtgc atcgaatcta
    30361 ccctcaccca ggttctggat cgccgattcg agttgggcga tcggcttgga aatcagcaac
    30421 gtcgccgcaa tgaccagcag tagcgcgatc ggcaccaatg ccagcagctg ccagtagatg
    30481 aagcgctgta cttcgccggc cattttctgc atggtgtcaa cttcgcggtc caccagcagg
    30541 ttgccttgct cgtcaagtgc gcgcgccacc tctgacaggg cggcaagctc cgccaccgcc
    30601 gaccggatta cgtccgcctt tgctcccgac tgtccaatgc gatcgaaaag tacagcctcg
    30661 cgggcgcgca atgcctcgag tgcctgtttc tgccgcgcat ccagcggcag cgtcgccatc
    30721 ctgcgggcgg tatccacgaa tcgttcgtgg gcgagtccgt aaccctgcag cagcgacggc
    30781 tcgccgatga ttgcgaactg cctgacgctg cgctccatgt tgttgatctg ctcgatcagg
    30841 aaccggctgt tctgggtggc ctgtaccgcc tggtaaaccg cgcgctggct atgtgtggca
    30901 agctggtgaa tcgagaaggc gttgttgatg agcgcgaaaa tgagcggcag gacggcaaga
    30961 gaaaagccgg ccagaatcaa cttcagaaac gagcgcgggt aataaagctg catacggact
    31021 catatcctct tggtcgccgt atcggtttgg gccgcttgcc agacctggtc ttcccgtgtc
    31081 ccacgcggag cggcgaatcc tcggatcacc cgagcgcgtc aatcgttgcg cccggacaac
    31141 gcctactatc gcatggaact cgtctccgtg ccacgcggac ccttgatttt atgggaaagt
    31201 tcgtctctgc tgctagggtg cgaggcgcgc ttcaacgcgt ccgaacagac caaaatccgc
    31261 gaccacgtga tcgccaggcg cgatctccac cggcaccagg caagtgcccg tcgttaccac
    31321 atgtccggcg cgtag
//
LOCUS       DASQOP010000182        12010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_78578, whole genome shotgun sequence.
ACCESSION   DASQOP010000182 DASQOP010000000
VERSION     DASQOP010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12010
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78578"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VE030_07565"
     CDS             <1..229
                     /locus_tag="VE030_07565"
                     /inference="COORDINATES: protein motif:HMM:NF018717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HZF23306.1"
                     /translation="GRVVSAGTGPLREMHGDTTRRQLIVVEFPSLEVFEQFIDEAENQ
                     NIHHIRENSTTDYIWTLYEPWDMKKWVKVES"
     gene            299..670
                     /locus_tag="VE030_07570"
     CDS             299..670
                     /locus_tag="VE030_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZF23307.1"
                     /translation="MSARTPDWKDESKLKALSSRYVKTDSLPWRKTAFPGVEIKVLLE
                     DKDSGLLTSLTRMAPGAVLPLHEHADVEQTYVIEGRLVDEEGEVAAGEYVWRPKGSTH
                     VATAPEGALLLGMFLRPNRFL"
     gene            complement(702..1691)
                     /gene="dhaK"
                     /locus_tag="VE030_07575"
     CDS             complement(702..1691)
                     /gene="dhaK"
                     /locus_tag="VE030_07575"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HZF23308.1"
                     /translation="MKKFLNTVDTVLSDSLRGFGQAHADIVAVNFEPNFVFRVNKPKG
                     KVALVSGGGSGHEPLHAGFVGYGMLDAACPGQVFTSPTPDQINAAAEAVNTGAGVLFI
                     VKNYSGDVMNFEMAAELFSGEHASVITNDDVAVENSTYTTGRRGVAATLVVEKIAGAA
                     AEAGTDLVACRALGERVNRRCGSMGVALTSCTVPAAGKPTFSLSEAEMEMGVGIHGEP
                     GRRRVAMTNADSIAAELVGAIVGNLKPKTGEPLLLFVNGFGGTPSMELYLMYDAAARQ
                     LQKRGLDIARSLVGNYVTSLEMAGCSITLSRMDEEMLRLWDAPVHTAALRWGR"
     gene            complement(1691..2137)
                     /locus_tag="VE030_07580"
     CDS             complement(1691..2137)
                     /locus_tag="VE030_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23309.1"
                     /translation="MAETASATEKIAAYKTILKEYIDRRPSGMRQKLAAALGKHKSFV
                     SQITGPSYAMPIPVAHLQTIFEICHFSPAERKAFLAAYSAAHPARRKPLQNVAAPARI
                     AHRILQLEIPLLADPGQQKAAEELLKEFAGRVFALIQSSQSRNGRS"
     gene            complement(2148..4067)
                     /gene="ptsP"
                     /locus_tag="VE030_07585"
     CDS             complement(2148..4067)
                     /gene="ptsP"
                     /locus_tag="VE030_07585"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HZF23310.1"
                     /translation="MTSPGSSIAVNEAAEGAAVINNHSGLHARPAVKLTKLAKRFSAA
                     VRMRIGPHGDWVDAKSVVKVMGMRAAMGEMLYFSADGPDAANAVQALLELTRRDFDEG
                     SPAERQFKGQVASSGFAVGVIARPVRAIAIRGGTIEPKISRSQLERAIACASRDLEGL
                     LTASDRMASEILEFQLEFLKDTKLTEPAFEAITQGSAAAEAWTRTVDRELAGYRAADN
                     EYFRARTSDLADLRDRVLAALNPDAAAEVEIPEGAVYLAEDLPPSRFLELDWSKYRGA
                     ALLGGSVASHVSMLARSRGVPLLVQVAPEAGELLNGAPVILDAEGGHLVAYASGSSLQ
                     SAQKRISARETKRQAADFYRTLPAIGRGGQRIRVYINVEDPEQLTRVDPAECDGIGLV
                     RTEFLFRGVQGLPDEETQFQFYRRLVEWARGRPVTIRTLDAGGDKPIAGLTPENETNP
                     FLGVRGVRLSLARPEVFRQQLRALARVAVLGPVRVMLPMVTVPGELEKAREMLAEEVD
                     NLTRDGIPTRMPELGMMVEVPAAALTAAAFDADFYSIGSNDLIQYTMAASRDCSGVAN
                     LYDARNGAVLELIARVAAHGAASGRDVSLCGEMASDPTAIAPLLDAGLTALSVTPVAL
                     AQVKFAIAEHGRKTE"
     gene            complement(4064..4453)
                     /gene="dhaM"
                     /locus_tag="VE030_07590"
     CDS             complement(4064..4453)
                     /gene="dhaM"
                     /locus_tag="VE030_07590"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: protein motif:HMM:TIGR02364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HZF23311.1"
                     /translation="MNNNVAIVIVSHSIDVAKGTADMVRQMVGDEVRVAWCGGNPDGG
                     LGTSMPDIKRCIETVYGPRGVAVLVDLGGAETNSEMAIEMLPGQWQQNVVICNAPIVE
                     GAVMAATEAAGGSDLAKVRATAEELSP"
     gene            complement(4450..5100)
                     /gene="dhaL"
                     /locus_tag="VE030_07595"
     CDS             complement(4450..5100)
                     /gene="dhaL"
                     /locus_tag="VE030_07595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HZF23312.1"
                     /translation="MRTGIPSTQLMAHLIEAMAGTIDAHVELLTGLDQAIGDGDHGLN
                     MKRGFAAIHEAAEELAALAPPEALLKAGLILVSKVGGASGPLYGSLFMAMGKSGAGRT
                     LDRATVVNMLADGVAAIKKRGKSEAGEKTMLEVLVPVLAALSDGVANDVRLAPLLDQV
                     RQAARGGLEASRPLLATKGRASFLGDRSIGHLDPGAQSSYLLVDAVCNVFEEGATQ"
     gene            complement(5104..6117)
                     /locus_tag="VE030_07600"
     CDS             complement(5104..6117)
                     /locus_tag="VE030_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZF23313.1"
                     /translation="MAEISLQSLNKRFGDTQAIADFTLTVADGEFFVLLGPTGAGKTT
                     LLRLAAGLERADSGSIAIGATDVTRMQPAHRDVTFVFQQYSLYPHYTVFDNLAFPLRS
                     PRHRLPENQIARKVNQVAELLGIGEKLQNRATRLSGGEMQRVAIGRALVRNPKAYLMD
                     EPLSSLDAKLRADLRVELKRIQADLGATIIYVTHDQMEAMTLANRIGVMNQGRLIQVA
                     TPREIYSEPEDTYVACRLGSPRINLVPAQMLPTVDTPPGTVTIGVRPEDVVLGGDGPH
                     GQVSLVETLGAETVIEIDCAGEEVRALLAHGQRFHSGETVRIAPRAGRAIFFDAGGRR
                     IAA"
     gene            complement(6110..7207)
                     /locus_tag="VE030_07605"
     CDS             complement(6110..7207)
                     /locus_tag="VE030_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZF23314.1"
                     /translation="MAEIRIENLHKSFAHFTAVRDSTMTIGNGQFFVLLGPSGCGKTT
                     TLRMIAGLEIPTSGKILLGGDDVTFRRAAQRDIAFVFQFFALYPHMNVRENLAFPLKT
                     QLTARAETRRRVDEVAQMLRIEHLLPLPVGGLAGGDRQRVALGRAIIRRPKAFLMDEP
                     LGALDAEFREMMCGELRRLHDRIGATTVYVTHDQTEAMAMGDRIAVMNKGEVLQAGPP
                     QDVYDRPRNMFVAGFIGSPAMNFLPIDGGAANGAHEVRVAGAHISVPRLHAGVKEGKG
                     VIGVRPEHIRIDANGALRGNVFAVEYMGSTQLVTVDTPAGRLRLKAPNTIQVEMRSTV
                     GLQMDATRVVVFDKDSEQALPSDLINGNGHG"
     gene            complement(7210..7392)
                     /locus_tag="VE030_07610"
     CDS             complement(7210..7392)
                     /locus_tag="VE030_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23315.1"
                     /translation="MRNLRRWFRRGPWEGAATLLIGLGVVMLMQPFSKFAYANGFTVL
                     LMGTIGFVIVSHFPEQ"
     gene            complement(7389..8375)
                     /locus_tag="VE030_07615"
     CDS             complement(7389..8375)
                     /locus_tag="VE030_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HZF23316.1"
                     /translation="MDRAAAEVATVPAGHSTARNPTESSPAVKWLAGIVVAIYAVITL
                     IPVAWIAMTSFKDVTEAVAYPPVVFFEPTLEGYVNLFTTRTRATPEYMESLPPPATWY
                     DRIVRSRNMVIAGPSPFAGRFLNSVIIGFGSTFLAVFLGTLAAYAFSRFRVPLKDDLM
                     FFILSTRMMPPIAVAIPIFLMYRQLGLADTHLGLILLYTAINVSFAVWLLKGFIDEIP
                     REYEEAAMIDGYTRLQAFVKVVLPQAATGIAATAIFCLITAWNEYAFALLLTTRTAQT
                     APPFIPNVIGEGGLDWAAVSAGTMLFLLPVMIFTLLLRKHLLRGITFGAVRK"
     gene            complement(8380..9306)
                     /locus_tag="VE030_07620"
     CDS             complement(8380..9306)
                     /locus_tag="VE030_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HZF23317.1"
                     /translation="MPSSVPADRLATAAVSAGLSDRVITWLFVAPTIVLLLAINIFPL
                     FWTIYLSFTNFRANQPGRAVEWVGLDNYRSLFDSDAVWSYLQNTAHFVVWSITLQVLI
                     GFGLALLLHRQFRGHATLTTLILLPMMLSPALVGLFWRYLFDPQAGIFNYVVNYFGGF
                     GTFSMLSSTQLAPWAIILVDTWMWAPYVMLLCLAGLRSVPPYLYEAAEVDRASRLQQF
                     WHITLPMVMPLLMVAVLFRAIECFKVFDMVIELTSGGPGETTELASLSLKRAAFEKWR
                     TGYASAFAILLFVVVFGASNLYVKYLNRVKSR"
     gene            complement(9417..10739)
                     /locus_tag="VE030_07625"
     CDS             complement(9417..10739)
                     /locus_tag="VE030_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZF23318.1"
                     /translation="MSRMKLALLAAALGLSFSVAQAQEKSITVGWAAWDPANALIELS
                     KDFTKETGIAVRGEFIPWPTFQEKIFTELNAKGSTYDVLIGDSQWIGAGATYGHYIEL
                     TDFFKSNKIDLEKDFAPLPVFAYSTWPKGSKHYWALPAEGDGLAFTYRKDWFERADLR
                     AEFKKQYQYDLAAPKTWEQLRDIGKFFQGRTIDGKKVYGMAINTKRAGDGITMGVTNT
                     LYTWGTQYENPKKPYDLDGYFNSKASAEALEFYKTLYDCCTPPGWGDAYMQENLDAYT
                     SGQVAMAMNWYAFFPGISKHPAVGKVTGYFSMPSHSGKSSATLGGQGMSIVKYSKKQD
                     LAKEYLKWFARPEVQKKWWALGGYSCHNVVLNDPNFEKSAAYARGFKETMLKVRDFWQ
                     EPVYAELLDSSQKNFHIYVVEGKGTAQSTLDNIVKEWKDVFKADGKYN"
     gene            complement(11168..11620)
                     /locus_tag="VE030_07630"
     CDS             complement(11168..11620)
                     /locus_tag="VE030_07630"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZF23319.1"
                     /translation="MTTRICISAVIDAPIDKVWAKVRDFNNLPEWHPKFSRSHIEGGL
                     PSDQVGCVRNFDIAAGGGTIRERLLALSDTEHYFRYCILTSPLPVRDYVAQLTLYPVT
                     MGNKTFGIWIAECQVTHGDEAGLVDAVGNGTFGKAFEVLNEFFARQRH"
     gene            complement(11630..>12010)
                     /locus_tag="VE030_07635"
     CDS             complement(11630..>12010)
                     /locus_tag="VE030_07635"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZF23320.1"
                     /translation="RDFNGLPKWHPAIGKSAIEGGLPGDRVGCVRRFTLKKDGGLLRE
                     KLLTLSDSARELTYNILVSPMPVANYVATMRVTPVTVGNRSFAEWWAEFDVTSGPEHV
                     QVSDIGDNVFVAGFAAMNRKLARK"
BASE COUNT         2226 a         3756 c         3739 g         2289 t
ORIGIN      
        1 cgggagggtg gtgagcgccg gcaccggtcc gctgcgcgag atgcacggcg ataccactcg
       61 ccgccagctg atcgtggtcg agtttcccag cctggaagtg ttcgaacagt tcatcgacga
      121 ggcggagaat cagaacattc atcacattcg cgagaattcg accaccgatt acatctggac
      181 cttgtatgag ccgtgggaca tgaagaaatg ggtaaaggtg gaaagctaat gggtccgcga
      241 caaaacacaa cccttcgcaa gctccaacac agatgtagca accaaacgga ggacgatcgt
      301 gagcgcacgc acaccagact ggaaagatga aagcaagctg aaggcgcttt cgtcacgcta
      361 cgtcaagacg gattcactgc cgtggcgaaa gacggccttc cccggagtgg agatcaaggt
      421 gctgctcgaa gacaaggaca gcggattgct gacttcgctc acgcgcatgg ccccgggcgc
      481 agtgctgccg ttgcacgaac acgccgatgt ggagcagacc tacgtgatcg agggacgcct
      541 ggtggacgaa gagggcgagg tcgcggccgg ggaatatgtc tggcggccga agggcagtac
      601 ccacgttgcg accgcgccgg aaggagcgct gctcctcggc atgttcctgc ggccgaatcg
      661 ctttctctga tccgccgccg ggttacgccc gatccatgag cttagcggcc ccagcgcaat
      721 gctgcggtgt gtaccggcgc atcccatagc cggagcattt cctcgtccat gcggctcagg
      781 gttatcgagc atccggccat ctcaagagaa gtgacatagt tgccgaccag cgaacgcgcg
      841 atgtccagtc cacgcttctg caattgtcgg gcagccgcgt cgtacatcag gtaaagctcc
      901 atcgagggcg tgccgccgaa gccgttcacg aataacagca gcggctcgcc cgtcttcggt
      961 ttgagattgc cgacgattgc gccgaccagc tcggcggcaa tcgaatcggc gttggtcatc
     1021 gcgacgcggc ggcgacccgg ttctccgtgg atgcccacgc ccatttccat ctccgcttcg
     1081 gaaaggctga atgtcggctt cccggcggca gggaccgtac acgaagtcag cgccacgccc
     1141 atcgagccac agcgccggtt gacgcgttcg ccgagcgccc ggcaggccac cagatcggtg
     1201 cccgcctcgg cggctgcgcc cgcgatcttt tccaccacca gcgtggcggc cacgccacgc
     1261 cttccggtcg tatacgtgga gttctcgact gcaacatcgt cgttggtgat tacgctggcg
     1321 tgctctccgg agaacaactc ggcagccatc tcgaaattca tcacgtcgcc ggaataattc
     1381 ttgacgatga acagcacgcc ggcaccggtg ttcactgctt ctgcagcggc attgatctgg
     1441 tccggggtgg gggaggtgaa cacctggccg ggacaggcgg catcgagcat gccatagccg
     1501 acgaagccgg cgtgcagcgg ttcatgaccg ctcccgccgc cggaaacgag agccactttg
     1561 cctttgggct tgttcacccg gaacacgaaa ttcggctcaa agttcaccgc aacgatgtcg
     1621 gcatgggcct gcccgaaacc gcgcaggctg tcgctcagga ccgtgtcgac cgtgttgagg
     1681 aatttcttca tcaggacctc ccgttccttg attggctgga ctggatcagt gcaaataccc
     1741 tgccggcaaa ctccttgagc agttcctcgg ctgccttttg ctggcccggg tccgcaagca
     1801 gcgggatctc cagttgcaga atcctgtgcg caatgcgtgc cggtgcggcg acgttctgca
     1861 gcggcttgcg gcgcgccgga tgggcggccg aataagcggc aagaaaggct ttgcgttccg
     1921 ccggcgaaaa gtgacagatt tcgaatatcg tctgaaggtg cgcaaccggg atcggcattg
     1981 cataggaagg cccggtgatc tgcgagacga agcttttgtg cttgcccagg gcggccgcga
     2041 gcttctgccg catcccggag ggcctgcggt cgatgtattc cttgagaatg gtcttgtaag
     2101 cggcgatttt ctccgtggcc gaagccgttt ccgccatacg cgcccggtta ctccgttttc
     2161 ctgccatgtt ctgcgatcgc aaatttgacc tgggccagcg ccacgggggt taccgatagc
     2221 gcggtgagcc cggcatcgag cagcggcgca atcgccgtcg ggtcggatgc catttctccg
     2281 cacaggctga catcgcgccc gctggcggcg ccgtgcgccg ccacgcgcgc gatcagctcc
     2341 agcactgcac cattgcgcgc gtcataaagg ttggcgacac cagaacagtc gcggctcgcc
     2401 gccatggtgt attggatcag gtcgttgctg ccgatcgaat agaaatcggc atcgaacgcc
     2461 gcagctgtga gggccgccgc cggaacctcc accatcatac cgagctccgg catgcgtgtc
     2521 gggatgccgt cacgggtgag attgtcgacc tcttcggcga gcatttcccg cgccttctcc
     2581 aattccccgg gcaccgtgac catcggtagc atgacgcgca ccggacccag cactgcaact
     2641 cgagccaggg cacgcagctg ctggcggaat acctccggcc ttgccaggct caggcgcacc
     2701 ccgcgcacgc cgaggaaggg gttggtctcg ttttccggcg tgagacccgc gatcggcttg
     2761 tctcctcccg catcgagggt gcgaatggtc accggccggc cacgcgccca ttcgaccagc
     2821 cgccggtaaa actgaaactg ggtttcctca tccgggagtc cctgtacgcc gcgaaacagg
     2881 aactcggtgc ggacgagccc gatgccgtcg cattcagccg gatcgacgcg ggtcaattgc
     2941 tccggatctt ccacgttgat atacacgcgt atacgctgac cgccgcggcc gattgccggc
     3001 agcgtccgat agaaatcggc ggcctgacgt ttggtttcac gcgcggagat tcgcttctgt
     3061 gcggactgca gactggatcc tgaagcgtac gcaaccagat ggccgccttc ggcatcgagg
     3121 atgaccggcg cgccgttcag gagctcgccc gcctcgggtg ccacctgcac cagcagcggc
     3181 acgccgcgcg agcgcgccag catcgacacg tggctggcga cgctcccgcc gagcagcgcc
     3241 gctccccgat atttgctcca gtccaactcg aggaacctcg acggcggcag gtcctcagcc
     3301 aggtagaccg ctccttcggg aatttcaact tccgcagccg catccgggtt caacgccgca
     3361 agcacccggt cccgcagatc cgcgaggtcg ctggttcggg cgcggaagta ttcgttgtct
     3421 gcggcacggt aaccggcaag ttcgcgatcc acagtcctgg tccaggcctc ggcggcagca
     3481 ctgccttggg tgatcgcttc gaacgcgggc tcggtcaatt tggtgtcctt caggaactcg
     3541 agctggaatt cgagaatttc agatgccatg cggtcgctgg cggtcaacag cccttcaaga
     3601 tcgcggctgg cgcaggcgat cgcccgctcg agctggctgc gcgagatttt cggctcgatg
     3661 gtgccgccgc gaatggcgat cgcgcggacc gggcgggcga tcacgccgac ggcaaatccg
     3721 ctggatgcaa cctggccctt gaactggcgt tcggccggcg agccttcatc gaaatcgcgg
     3781 cgggtgagtt cgagcagcgc ctgcaccgcg ttcgcggcgt ccggcccgtc ggcggagaaa
     3841 tacagcattt ctcccatggc tgcgcgcatg cccatcacct tgaccacgct cttggcgtcg
     3901 acccagtcgc cgtgcggccc gatacgcatc ctcaccgccg cgctgaagcg cttggccagc
     3961 ttggtgagct tgaccgcggg ccgggcgtgc aggccgctgt gattgttgat gaccgcggcg
     4021 ccttcggctg cctcgttcac cgcgatgctt gatcccggac tggtcatggc gacaattctt
     4081 ctgcagtggc ccggaccttg gcgagatcgc tgcctccggc tgcctcggtg gcagccatga
     4141 ccgcaccctc gacgatcggc gcattgcaga tgacgacatt ctgctgccac tgtccgggca
     4201 gcatttcgat cgccatttcc gagttcgttt ccgcgcctcc caggtccacc agcaccgcca
     4261 cgccgcgagg cccgtagacg gtctcgatgc agcgcttgat atccggcatg ctggtgccca
     4321 ggccgccgtc cggattgcct ccgcaccagg caacgcggac ctcgtcgccg accatctggc
     4381 gcaccatatc ggccgtcccc ttggccacat ctatcgagtg ggaaacgatg acgatggcca
     4441 cattgttgtt cattgcgtcg caccctcttc aaagacgttg cagaccgcgt ccacgagcag
     4501 gtaactgctc tgcgcccccg ggtcgagatg accgatcgac cgatcgccga ggaacgaggc
     4561 gcgccctttg gttgccagca acgggcggct ggcttccagg ccgccgcgcg ccgcctgcct
     4621 cacttgatcg agcaaaggcg cgagccgcac atcgttggct acgccgtcgc tgagcgcggc
     4681 gagcaccggc accagaactt ccagcatggt cttctccccg gcttccgact tgccgcgttt
     4741 ttttatcgcc gccacgccgt ccgcgagcat gttcacgact gtggcgcggt ccagcgtacg
     4801 gcccgccccg gacttgccca ttgccatgaa caggctgccg tagagcggac ccgaagcgcc
     4861 gccgactttt gaaaccagga tcaacccggc cttcagcagc gcctcgggtg gcgccagtgc
     4921 ggcgagttcc tccgcggctt cgtgtatggc ggcaaagcca cgtttcatgt tcaggccatg
     4981 gtctccgtcg ccgatcgcct gatcgagtcc ggtcagcaac tccacatgcg cgtcaatggt
     5041 ccctgccatc gcttcgatga gatgtgccat caattgcgtg gaaggaattc ctgtacgcat
     5101 ttttcaggcg gcgatgcggc gcccgccggc gtcgaagaat atcgcgcggc ctgcgcgcgg
     5161 ggcgatacgt accgtctcgc cgctgtgaaa cctctgcccg tgcgcaagca gtgcccgtac
     5221 ttcctcaccc gcgcagtcga tctcgatcac ggtttcggcg cccagagttt ccaccagtga
     5281 aacctgtccg tgcggcccgt cgccgccgag gaccacgtct tcggggcgca cgcctatggt
     5341 caccgtgccc gggggtgtgt ccacagtggg cagcatttgc gccggaacca gattgatgcg
     5401 cggcgagccg agccggcagg caacgtaggt gtcctccggc tcggagtaga tctcgcgcgg
     5461 ggtcgccact tgaattagcc ggccctggtt catcaccccg atccggttcg ccagcgtcat
     5521 tgcctccatc tggtcatgcg tcacatagat gatggtcgcg cccaggtccg cctggatgcg
     5581 tttgagttcc acgcgcagat ctgcccgtag cttggcatca agcgaggaca gcggttcatc
     5641 catcagatag gccttcgggt tgcgtaccag tgcgcggccg attgccacac gctgcatttc
     5701 cccgccggaa aggcgcgtgg cgcgattttg cagcttctcg ccgatcccga gcagctcggc
     5761 tacttggttt accttgcgcg cgatctggtt ctcagggagc cggtgccgag gggagcgcaa
     5821 ggggaaggcg agattgtcga ataccgtgta atgcggatag agcgaatact gctggaacac
     5881 gaaagtgacg tccctgtgcg ccggctgcat gcgggttaca tccgttgccc cgatcgcaat
     5941 gcttccggag tcggcgcgtt ccagcccggc cgcgagccgc aacagagtgg ttttgcccgc
     6001 gccggtaggg ccgagcagga cgaagaactc gccgtcggcg acagtcaggg taaagtccgc
     6061 gattgcctgc gtatcgccga agcgcttgtt gagggactgc agcgagatct cagccatgcc
     6121 cgttcccatt gatcaggtcg cttggcaggg cctgctcgct gtccttgtcg aaaacgacca
     6181 ccctggttgc gtccatctgc aggccgaccg tgctgcgcat ttcgacctga atggtgttcg
     6241 gcgctttcag gcgcaggcgt cctgcgggcg tgtcgacggt gaccaactgc gtcgatccca
     6301 tgtattcgac cgcaaacacg ttgccgcgca gcgctccgtt tgcatcgatg cgaatgtgtt
     6361 ccgggcgtac accgattacg cctttccctt ccttgacgcc ggcatgaagg cgcggcacgc
     6421 tgatgtgcgc accggcaacc cggacttcat gcgcaccatt ggcggcgcca ccatcgatcg
     6481 ggaggaagtt catcgccggg ctgccgatga acccggcgac gaacatgttg cgcggccggt
     6541 cgtacacgtc ctggggcggg ccggcctgca gcacttcccc cttgttcatt acggcaatgc
     6601 gatcacccat ggccattgcc tcggtctggt cgtgggtcac gtacaccgtg gtggcgccaa
     6661 tgcggtcgtg cagccggcgc agttcaccgc acatcatttc gcggaactct gcatccagtg
     6721 caccgagcgg ttcgtccatc agaaatgcct tcggccggcg gatgatcgcg cgtccgagcg
     6781 ccacgcgctg gcggtcgccc cccgccaaac cgccgaccgg cagcggcagg aggtgttcga
     6841 tgcgaagcat ctgtgcgacc tcatcgacac gcctgcgggt ctcggcgcgc gcggtgagct
     6901 gggtcttcag cggaaaggcg aggttctcgc gcacgttcat gtgcgggtac agcgcgaaga
     6961 actggaacac gaaggcgatg tcgcgttgcg ccgcgcggcg gaaagtcaca tcgtcgccgc
     7021 caagaaggat cttcccggag gtggggattt cgaggccggc gatcatgcgc agggtcgtgg
     7081 tcttgccgca tcccgaaggg ccgagcagga cgaagaactg cccattgccg atggtcatgg
     7141 tgctgtcgcg caccgccgtg aaatgcgcga agctcttgtg caggttctcg atgcggatct
     7201 cagccattct cattgctccg gaaaatgact gacgatcacg aagccgatgg tgcccatcag
     7261 cagcaccgtg aagccattgg cgtacgcgaa cttggagaaa ggctgcatca gcatgaccac
     7321 gcccaaaccg atcagcagcg ttgccgcccc ctcccatggt ccgcgccgga accagcgccg
     7381 cagattcctc atttgcgcac cgcgccgaag gtgatgccgc gcagtaaatg cttgcgcagc
     7441 agcagcgtga agatcatcac cggcagcagg aacaacatgg tgccggcgga aaccgccgcc
     7501 cagtccagac ctccttcgcc gatgacgttg ggaatgaagg gcggcgccgt ctgcgcggtg
     7561 cgtgtggtaa gaagcagcgc gaatgcgtac tcgttccagg cggtgatcag gcagaagatc
     7621 gcggtggcgg cgatgccggt ggcagcctgc ggcaggacca ctttgacgaa ggcttgcagg
     7681 cgcgtgtaac cgtctatcat cgctgcctct tcatactctc tggggatctc gtcgatgaag
     7741 cccttgagca gccacacggc aaagctcacg ttgattgcgg tatacagcag gatcaggccg
     7801 aggtgcgtgt cggcaagccc cagttggcgg tacatcagga agatcggaat ggccaccgcg
     7861 atgggcggca tcatccgggt cgagaggatg aaaaacatca ggtcgtcctt cagcggcacc
     7921 cggaagcgcg agaaggcgta ggcggcaagc gtgcccagaa acaccgcaag aaaagtggac
     7981 ccgaagccga tgatcacgga attcaggaag cgtccggcaa aaggagacgg gccggcgatc
     8041 accatgttgc ggctgcggac gatgcggtcg taccaggtcg ccggcggcgg caggctttcc
     8101 atgtactcgg gcgtggcgcg ggtgcgtgtg gtgaacagat tgacgtaacc ctccagcgtc
     8161 ggttcgaaga acaccaccgg cggataggcc actgcctcgg tcacgtcctt gaacgaggtc
     8221 atcgcgatcc aggccaccgg gattagcgtg atcactgcat agatcgccac cacgatgccg
     8281 gcaagccact tcacggccgg cgaggattcg gtcgggttcc gcgccgtcga atgtccggcg
     8341 gggactgtgg cgacctcggc tgcggccctg tccattttcc tatctcgatt tgacacggtt
     8401 cagatacttc acgtacaggt tggacgcgcc gaataccacc acgaacaaca ggatcgcgaa
     8461 ggcggacgcg taaccggtgc gccacttttc gaaggcggcg cgcttgagcg acagcgaggc
     8521 cagctcggtg gtttcgccgg ggccgccgct ggtgagctcg atcaccatgt cgaacacctt
     8581 gaagcactcg atggcgcgga acaatacggc gaccatcaga agcggcatca ccatcggcag
     8641 cgtgatatgc cagaactgct gcagcctcga ggcgcgatcc acctcggccg cctcgtagag
     8701 ataaggcggg accgaacgca gcccggcaag gcacagcagc attacgtaag gtgcccacat
     8761 ccaggtgtcc accaggatga tcgcccacgg ggcgagctgg gtgctcgaca acatgctgaa
     8821 agtgccgaag ccgccgaaat aattcacgac gtaattgaag atccccgcct gcggatcgaa
     8881 aaggtaacgc cagaacaacc cgacgagcgc gggcgacagc atcatcggca gcaggatcag
     8941 ggtagtgagc gtggcgtggc cgcggaactg ccggtgcagc agcagcgcca ggccgaagcc
     9001 gatcaggacc tgcaaagtga tcgaccacac gacgaaatgc gcggtgttct gcaaatacga
     9061 ccacaccgcg tcggaatcga acagcgaccg gtagttgtca agacccaccc attccacggc
     9121 acggccgggc tggttggcac ggaaattggt gaaactgaga tagatggtcc agaacagcgg
     9181 aaaaatattg atcgcaagca gcaggacgat ggtcggcgcc acgaacagcc aggtgatgac
     9241 acggtccgac aggccggcgg ataccgccgc agtggcgagg cgatcggctg gaacggaact
     9301 gggcatggtc agtacgtccg ggttggaagc gcgccgggac catcccggcg cgcccgttgc
     9361 attgtgccgg aacaggcgcg aacccggctg cgatccgcgc gatccgtcct tccaactcag
     9421 ttgtacttgc cgtccgcctt gaaaacgtcc ttccactctt tgactatgtt gtcgagcgtg
     9481 ctttgcgccg tgcctttgcc ctcaacgacg tagatgtgga agttcttctg gctgctgtcg
     9541 agcagttcgg cgtacaccgg ctcctgccag aaatcgcgca ccttgagcat ggtctccttg
     9601 aagccgcggg cgtaggccgc gctcttttcg aagttcgggt cgttcaacac cacgttgtgg
     9661 caggaataac cgcccagtgc ccaccacttc ttctggactt cgggacgcgc gaaccacttc
     9721 agatattcct ttgccagatc ctgcttcttc gagtacttga cgatcgacat gccctgtccg
     9781 ccgagcgttg ccgaagactt gccgctgtgc gacggcatcg agaagtagcc ggtaaccttg
     9841 cctacggccg gatgtttgct gattccgggg aagaacgcat accagttcat ggccatcgcc
     9901 acctggccgc tggtgtaggc gtcgaggttc tcctgcatgt aggcatcgcc ccagcccggt
     9961 ggggtgcagc aatcgtacag cgtcttgtag aattccagcg cttccgccga cgccttgctg
    10021 ttgaagtagc cgtccaggtc atagggcttc ttcggatttt cgtactgcgt tccccatgta
    10081 tagagcgtgt tggtcacgcc catggtgatg ccgtcgcctg cccgcttggt gttgatggcc
    10141 atcccataaa ctttcttgcc gtcgatggtg cggccctgga agaacttgcc gatgtcgcgc
    10201 aattgctccc aggtcttcgg tgccgcgaga tcgtactgat actgtttctt gaattcggct
    10261 ctcaggtccg cgcgttcaaa ccagtccttg cggtaggtga acgcgaggcc atcgccttcc
    10321 gccggcaggg cccagtagtg cttggacccc ttcggccagg tggagtaggc aaacaccggc
    10381 agcggcgcaa agtccttctc caagtcaatc ttgttgctct tgaagaaatc ggtgagctcg
    10441 atgtagtgac cgtaggtggc gccggcgccg atccattggg aatcgccgat caggacgtcg
    10501 taggttgaac ccttggcgtt cagttcggtg aagatcttct cctggaaggt cggccaggga
    10561 atgaactctc cccgtactgc aatgccggtt tccttggtga agtccttgga cagttcaatc
    10621 agggcatttg ccgggtccca ggcggcccaa ccgacagtga tcgacttctc ctgggcctgt
    10681 gccacggaaa acgaaagtcc cagcgcggca gcaagcagtg ctagcttcat cctactcatg
    10741 gtgaatcctc cttggtgatg gtgaacttcg aacgcgcggg gaggagacag ccggctacgg
    10801 ctgttgggcg gaaattcctc ccccggtctt ggttcgggcg gtttagtttc ttggtttagt
    10861 tttgccagaa atataaacta aactaaactt tcgtcaagca gattttgtcg ggtcgggtga
    10921 cccgggtgat ccttggtgcg gcgcgcaatg cgctgggccg tcgacagtcg gatctcggtc
    10981 ctggccattt ccggagcggc aaaggcaggc gcggaaagcg ctgaacaaaa cagtttagta
    11041 aggtttagta acgacgcaaa tcttgcgctg cagcagcctg attttttatt gcccgggacc
    11101 ttctgtgagg ctctatgacg actgcgcagt tgctgaatca gtcgccggag atgggtctgg
    11161 ggagggctta atgccgctgc ctggcgaaga actcattcaa aacttcgaag gccttgccaa
    11221 aggtgccgtt gccgaccgcg tccacgagac cggcctcatc gccgtgggtg acctggcact
    11281 cggcgatcca gatgccgaag gtcttgttgc ccatggtgac cgggtagagc gtgagctgcg
    11341 ccacgtagtc ccttaccggc agcggcgaag tgaggatgca gtatctgaag taatgctcgg
    11401 tgtcggacag cgccagcagc cgctcgcgga tggtgccgcc gccagcggcg atgtcgaaat
    11461 tgcgcacgca accgacctgg tcgctcggta gccccccttc gatatggctg cgcgaaaact
    11521 tcggatgcca ctctggcagg ttgttgaagt cgcgtacctt ggcccagact ttatcgatcg
    11581 gggcgtcgat caccgcactg atgcagatcc tggtggtcat ggcgcctccc tatttcctcg
    11641 cgagcttgcg gttcatcgcg gcaaacccgg cgacgaagac gttgtcgccg atatcgctca
    11701 cctggacgtg ctcgggtccg gaggtgacat cgaactcggc ccaccactcg gcgaaactcc
    11761 tattgcccac cgtgaccgga gtgacgcgca tggtcgccac gtagttcgcg accggcatcg
    11821 gcgaaaccag gatgttgtaa gtcagctccc gggccgaatc cgagagcgtc agcagttttt
    11881 cgcgcagcaa cccgccatcc ttcttgaggg taaagcggcg cacgcagccg acgcggtctc
    11941 ccggcagccc gccttcgata gccgattttc cgatcgccgg gtgccacttc ggcagtccgt
    12001 tgaagtcccg
//
LOCUS       DASQOP010000183         2097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_884668, whole genome shotgun sequence.
ACCESSION   DASQOP010000183 DASQOP010000000
VERSION     DASQOP010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884668"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /gene="soxC"
                     /locus_tag="VE030_07640"
     CDS             complement(<1..1070)
                     /gene="soxC"
                     /locus_tag="VE030_07640"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR04555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HZF23321.1"
                     /translation="MKSLVAVAGNGLLDRRAFLRGGAALAAAMTGYTLAGSAAAAPLA
                     DDPWSRAPGTNVPAYGVPSRFEKSVVRTLSNPNGEPRTQHGRTPHHLLHGTSTPNGLH
                     FVISHAGDADIDPEKHRLVIHGLVKRPLLFGLDALARYPMVSRMTFVECGGNSAPLFS
                     REPIQASVQALHGLASCAEWTGVMLSTLLEETGVDPKAKWLVAEGADAPALSRSVPIA
                     KALDDAMIALYQNGERLMPGNGYPLRLLLPGWEGNMNVKWLRRLKLVEAPAMSYYESR
                     TYAPILPNGKAYQFYFLQEVKSFITHPSPGLMLKAPGFYEISGIAYSGNGRISKVMVS
                     ADGGQSWAQAALHEPVLSKAFTR"
     gene            complement(1108..>2097)
                     /locus_tag="VE030_07645"
     CDS             complement(1108..>2097)
                     /locus_tag="VE030_07645"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZF23322.1"
                     /translation="ARQFWDDIVRWDCTLFQYIGELCRYLLRTDPLPHEAEHRVRMCC
                     GNGLRPDVWREFKSRFRIPRILEFYAATEGNVSLFNVEGKPGAIGRVPSFLAHRFPAR
                     LVKFDVEKDAPVRDEQGWCMRCAANEIGEAIGRIHADASNIAGRFDGYTSQEDSEKKI
                     LRSAFEPGDAWFRTGDLMRQDEAGYFYFVDRIGDTFRWKGENVATSEVSEAICEFRGV
                     TDANVYGVQIPGTEGRAGMAALVAEDDLDLAAFRAHLIARLPAYARPLFLRLRSRMEL
                     TATFKYTKHEFVRQGYDPAATSDLLYFDDPERQAFVRLDQALYERIQTGQFRL"
BASE COUNT          377 a          679 c          676 g          365 t
ORIGIN      
        1 cgcgtaaagg ccttgctgag caccggttcg tgcaaggccg cttgcgccca gctctggccg
       61 ccgtcggccg agaccatcac tttggaaatg cggccgttgc ccgaataagc gatgccggat
      121 atctcgtaga agcccggcgc cttcagcatc agccccggtg acgggtgggt gatgaaggac
      181 ttcacctcct gcaggaaata aaactggtac gcctttccgt tcggcaggat gggcgcgtac
      241 gtccgcgact cgtagtagct catcgcgggc gcctcgacca gcttcaggcg gcgcagccat
      301 ttcacgttca tgttgccttc ccagccggga agcagcaggc gcagcggata gccgttgccg
      361 ggcatcagcc gctcgccgtt ctggtagagc gcgatcatgg catcgtcgag cgccttggcg
      421 atcggcacgc tgcggctcag cgccggcgcg tcggcgcctt cggcgaccag ccatttcgcc
      481 ttggggtcga caccggtttc ctcgagcagg gtcgacagca tcaccccggt ccattccgcg
      541 caggaggcaa gcccgtgcag cgcctgcacg ctcgcctgga tcggctcgcg ggaaaacagc
      601 ggagcactgt tgccgccgca ttcgacaaag gtcatgcgcg acaccatcgg atagcgcgcc
      661 agcgcgtcga ggccgaacag cagcggccgt ttcaccaggc catggatcac caggcggtgc
      721 ttctccggat cgatatcggc gtcaccggcg tgcgaaatga cgaagtgaag gccgttcggg
      781 gtggacgtgc catgaagcag gtgatgcggc gtacggccgt gctgagttcg gggctcgccg
      841 ttcgggttgc tgagtgtgcg cacgacgctt ttctcgaacc gcgacggaac gccgtaagcc
      901 gggacgttcg tgcccggcgc cctgctccac gggtcgtcgg ccaggggcgc ggcggccgcc
      961 gatccggcca gcgtgtagcc cgtcatggcc gccgcgagcg cggcgccgcc gcggagaaag
     1021 gcgcgacggt cgagcagccc gtttcccgca accgctacaa ggcttttcat aatccctccc
     1081 cgttcgagca acataccgca aagccagtca caagcggaac tggccggtct ggatgcgctc
     1141 atagagcgcc tggtcgagcc gtacgaatgc ctggcgttcg ggatcgtcaa agtagaggag
     1201 gtcggaggtg gccgccggat cgtagccttg gcgcacgaat tcatgcttcg tgtacttgaa
     1261 ggtggcggtc agttccatcc tgctgcggag acgcaggaac aaaggacgcg cgtacgcggg
     1321 caggcgggcg atcaggtgcg cccggaatgc cgccagatcc aggtcatcct cggcgaccag
     1381 ggcggccatt ccggcccggc cctcggtacc cggaatctgc accccataga cattggcgtc
     1441 cgtcacgccg cggaactcgc agatcgcttc ggaaacctcg gaagtcgcga cattctcgcc
     1501 cttccagcgg aaggtatcgc cgatccggtc gacaaaatag aagtagcccg cctcgtcctg
     1561 ccgcatcagg tcgccggtgc gaaaccaggc gtctccaggc tcgaacgcgc tgcgcaggat
     1621 cttcttttcc gagtcctcct ggctggtgta accatcgaat cgtccggcga tattcgacgc
     1681 atccgcgtgg atcctgccga tcgcttctcc gatctcgttg gcggcgcagc gcatgcacca
     1741 tccctgctca tcgcggaccg gcgcgtcctt ctcgacgtcg aatttcacca gcctggccgg
     1801 gaatcgatgc gcgagaaacg agggaacgcg gccgatggcg cccggtttgc cttcgacatt
     1861 gaacagggaa acgttgcctt cggtggctgc gtaaaactcg agaatccgcg gaatgcggaa
     1921 gcgcgacttg aactcgcgcc atacatcggg tctcaacccg ttgccgcagc acatcctgac
     1981 gcggtgctcc gcctcatgcg gaaggggatc ggtacgcagc agataacgac acagctcgcc
     2041 gatgtactgg aacagcgtgc agtcccagcg gacgatgtcg tcccagaatt ggcgggc
//
LOCUS       DASQOP010000184         2858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2895593, whole genome shotgun sequence.
ACCESSION   DASQOP010000184 DASQOP010000000
VERSION     DASQOP010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2895593"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1775)
                     /locus_tag="VE030_07650"
     CDS             complement(<1..1775)
                     /locus_tag="VE030_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HZF23323.1"
                     /translation="MTVASANPLALSVLDRPNSYIGKVVPRPNLERLMQGRGLYVSDM
                     ELPRMAHVVFLRSPHAHARITGIDATAARRVPGVVAIVTGEQLAAVITPWVGVLSHLK
                     GLKSAPQHAIAIDHVCWQGEAVAAVVASSRAVAEDAAEIVSVEYQELDAVTDMRTALD
                     PETPVIHPSLGDNLAFERNLDAGAVDQAFADSDAVVEADFTFGRHTGVTLEPRAVVAD
                     WNAAEARLTIYQGTQAPHMVQNIAALHLGLEESQVRVVCKDVGGSFGIKVHIYADEMA
                     TYALSKMLRRPVKFVADRVESFNTDIHARDHRCKGRIGVKRDGTIMAFEIDDLTGIGP
                     YSMYPRTSAIEANQVVNLVGGPYVTRNYRARARVVFQNKNVMCQYRAVGHPIACSVTE
                     GLVDLAALRIGMDPVEIRRRNLIADDAYPCASPSGLRFEQLSHHAAINKLMAMMDYRS
                     LRAEQAALRIKNIHRGIGIASFIEVTNPSAAFYGVGGARISSQDGVAVRLDATGSVIC
                     QTSITEQGQGSESLTAQIVGSVLGVSMERVRVILGDTDNTPYGGGTWASRGAGIGGEA
                     ALQAAKILRKNILDVAAAILQSSPA"
     gene            complement(1772..2245)
                     /locus_tag="VE030_07655"
     CDS             complement(1772..2245)
                     /locus_tag="VE030_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZF23324.1"
                     /translation="MPISLDVNGEHVDAHVLPRLNLADFLREHLQLTGTHVGCEHGVC
                     GACTVRVNGEIVRSCLMLAVQTHGASVETIEGLSDSGEIADLQAAFRARNALQCGFCT
                     PAMLMAAQDLLKQHTEPDRERIREHLSGNYCRCTGYQAIVDAVETTALARMGRTR"
     gene            complement(2254..2856)
                     /locus_tag="VE030_07660"
     CDS             complement(2254..2856)
                     /locus_tag="VE030_07660"
                     /inference="COORDINATES: protein motif:HMM:NF015415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HZF23325.1"
                     /translation="MTEAIAHVAHPAIRNRGTLGGSLAHADPASELPACMVALNATMI
                     IRGQSGERRIAAENFFTGIYETALSPQELLVAVELPIGRKNSVHFFREFARRHGDYAI
                     VGLAAQAIVERDVFADLGLAFFAVGDRPVSARAAKKLINVAITPAVLSDASTALAEEL
                     DPQSDQQASASMRRHLAKVLLARCVAALLGRPDLNAGGPA"
BASE COUNT          536 a          932 c          879 g          511 t
ORIGIN      
        1 tcggcaggtg aggattgcag gatggcggct gcgacatcca ggatgttctt gcgcagaatc
       61 ttggccgcct gcaatgccgc ctcgcctccg atcccggcgc cgcgcgaggc ccaggtgccg
      121 ccgccataag gcgtgttgtc ggtatcgccc agaatgacgc ggacccgctc catcgagacg
      181 cccagcacgc tgccgacgat ctgcgccgtg agcgattccg agccctgtcc ctgctcggtg
      241 atgctggtct ggcagatcac cgagcccgtg gcgtccagtc gaacagcaac gccatcctgc
      301 gacgagatgc gggcgccgcc gacgccgtag aacgcagcac tcggattggt gacctcgatg
      361 aagctggcaa tgccgatgcc gcgatgaatg ttcttaattc gcaatgccgc ctgctcggcg
      421 cgcaagctcc tgtaatccat catcgccatc agcttgttga tcgcggcatg atgcgacagc
      481 tgctcaaacc tcagccctga gggcgatgcg cagggatagg cgtcatcggc aatgagatta
      541 cggcggcgga tttccaccgg atccatcccg atccgcaaag ccgccagatc gaccagtcct
      601 tccgtcaccg agcacgcgat cggatggccg acggcgcgat actggcacat cacgttttta
      661 ttctggaaca cgacgcgggc ccgcgcgcga taattcctgg tcacataggg gccgccaacc
      721 agattgacga cctggttggc ctcgatggcg ctggtgcgcg gatacatcga atagggaccg
      781 atgccggtga gatcgtcgat ctcgaaggcc atgatcgtcc cgtcccgctt gacgccaatc
      841 cgacccttgc agcgatgatc acgcgcatga atatcggtgt tgaagctttc gacccggtcg
      901 gcgacaaact tgaccggccg ccgcagcatt ttcgacagcg cataagtggc catttcgtcg
      961 gcgtaaatgt ggaccttgat gccgaaggag ccgccgacgt ccttgcagac cacgcgaacc
     1021 tgcgactcct caagcccgag atggagcgcc gcgatgttct gcaccatgtg cggcgcctgt
     1081 gtgccctggt agatcgtgag cctcgcctcg gcggcgttcc agtcggccac cacggcgcgc
     1141 ggctccaggg tcacgccggt gtgccgtcca aaggtgaaat ccgcttccac cactgcgtcg
     1201 gaatcggcaa acgcctggtc cacagcgcca gcgtcgaggt tgcgctcgaa ggcaaggttg
     1261 tcgccgagcg aaggatgaat gaccggggtt tccggatcga gcgcggtgcg catgtccgtc
     1321 accgcgtcca gttcctggta ttcaaccgag acgatttccg ccgcgtcctc tgcgacggcg
     1381 cggctgctcg ccacgacggc cgcgacggct tcgccctgcc agcagacatg gtcgatcgcg
     1441 atcgcatgct gcggcgccga tttgaggccc ttcaggtgcg acagcacgcc aacccacggc
     1501 gttatgacgg ccgctagctg ttcgcctgtc acgatggcga cgacgccggg cacgcgcctc
     1561 gccgcggttg cgtcgatccc agtgattctc gcatgggcat ggggcgagcg caggaatacc
     1621 acatgcgcca tccgcggcag ttccatgtcg ctgacataga gcccgcgtcc ctgcatcagc
     1681 cgctcaaggt tcggccgtgg gaccaccttg ccgatatacg aattcggccg atccagcacc
     1741 gagagcgcca agggatttgc ggatgccacg gtcatcgcgt gcgtcccatg cgcgccagtg
     1801 ctgtggtctc gaccgcatcg acaatggcct ggtagccggt gcagcggcag taattgccgg
     1861 aaagatgctc gcgtatccgc tcccggtctg gctcagtatg ctgtttcaat agatcctgcg
     1921 cggccatcag cattgccggc gtgcaaaacc cgcattgcaa cgcattgcgc gcgcgaaaag
     1981 cggcctgcag atcggcgatc tcgccgctgt cggataatcc ctcgatggtt tcgacggacg
     2041 cgccgtgggt ctgcaccgcg agcatcaggc aggaacgaac gatctccccg ttcacgcgca
     2101 cggtgcaggc tccgcaaacc ccatgctcgc aaccgacatg ggtcccggtc agttgcagat
     2161 gctctcgcaa gaagtctgcg agattgagcc gtggcaggac atgcgcgtcg acgtgctcgc
     2221 cgttgacgtc aagcgagatt ggcatcgcag ccgtcacgca ggtcctccgg cattgaggtc
     2281 ggggcggccc agaagcgcgg cgacgcaacg ggccagcaac accttcgcaa gatgccggcg
     2341 catggaagcc gaggcctgct gatcgctttg gggatcgagc tcttcggcga gcgccgtcga
     2401 tgcatccgac aacacggccg gcgtgatggc gacgttgatg agtttcttcg cggccctggc
     2461 tgatacgggt cgatcgccga cggcaaagaa cgccagacca agatcggcga acacatcgcg
     2521 ttcaacgatc gcctgagccg ccaggccgac gatggcgtag tcaccgtgcc gccgcgcgaa
     2581 ctcgcggaaa aaatgcacgg aattcttccg cccgatcggc agctcaactg cgaccagtaa
     2641 ttcctgcggt gacaacgccg tctcataaat tcccgtgaaa aaattctcgg ccgcaattcg
     2701 acgttcgccg ctctgacccc tgatgatcat ggtcgcattc agcgccacca tacaagccgg
     2761 caactccgac gcgggatcgg catgcgcaag gctgccaccg agggtccccc tgttgcggat
     2821 cgcggggtgc gcgacatgcg caatcgcttc cgtcagca
//
LOCUS       DASQOP010000185        10355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_79449, whole genome shotgun sequence.
ACCESSION   DASQOP010000185 DASQOP010000000
VERSION     DASQOP010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10355
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79449"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="VE030_07665"
     CDS             <1..793
                     /locus_tag="VE030_07665"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HZF23326.1"
                     /translation="WMQVAAGIIPAAVPRILGEDRETGMFAMQFLDSATYPVWKAQLR
                     DGEISADTARDVGHRIAAIHGRTAGDPAMALTFATDHIFFPIRLEPYLAATADKHPDC
                     AERLRSLMRVTGATRKALVHGDVSPKNILAGPDGPVFLDAECAWYGDPAFDLAFCLNH
                     LLLKCVWRPHWQARYLVCFDALAQAYLAAVTWESRTDIEARTAHLLPGLFLGRVDGKS
                     PVEYIDSDEGRERVRRVARALLSAPVDKLSDVRAAWQHEIATWNK"
     gene            801..2087
                     /gene="eno"
                     /locus_tag="VE030_07670"
     CDS             801..2087
                     /gene="eno"
                     /locus_tag="VE030_07670"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HZF23327.1"
                     /translation="MTDTTIARMHGRRVWDSRGRPTVEVEVHLASGFTGRAIAPAGAS
                     RGSNEAVDLRDGGTAFGGMGVSRAVAHVNGEISRLLIGRNALQQAAIDEELIALDGTP
                     GKSRLGGNAMIATSMAVLHAAAGAHGMPLYAYLAQGKGVRLPLPEIQIFGGGAHAGRR
                     VDIQDFMVMALGARSFDDAVAMTAEVYRAAGELMKEAGKLQGVADEGGFWPAFASNEE
                     ALTMLMRAIERAGYHPDEDIGISLDVAASELGRNGRYRLGLESRELDSDDLCELLLGW
                     LARYPIVSIEDPLAEDDAEGMKKFTAAAGSRAQIIGDDFLVTNAARVKNAAAAGACNA
                     VLIKPNQAGTVTETKAALDAGKRAGWGTIVSARSGETEDVTIVHLAVGWDAGQLKVGS
                     FARSERMAKWNEVLRIEEALGANAVFAGRAVLPRLA"
     gene            2245..2388
                     /locus_tag="VE030_07675"
     CDS             2245..2388
                     /locus_tag="VE030_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23328.1"
                     /translation="MTTDHVHHRRTARGILLILAAVFMFSGMVTLGFMRRLSDASDQS
                     GTH"
     gene            2402..2848
                     /locus_tag="VE030_07680"
     CDS             2402..2848
                     /locus_tag="VE030_07680"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZF23329.1"
                     /translation="MNRPAPENRNSYRHFQSIPTRWMDNDVYGHVNNVVYYSYFDTVV
                     NQYLIEQRALNIDTSKVIGLVVETQCRYFASITFPDVVHAGLRVATLGNSSVRYEIGL
                     FRNDEQTASAQGYFIHVYVDRATRRAVSLPADLRAALERIPIQSKP"
     gene            complement(3015..3524)
                     /locus_tag="VE030_07685"
     CDS             complement(3015..3524)
                     /locus_tag="VE030_07685"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HZF23330.1"
                     /translation="MPHIRDERPRDEAAIHAVVAQAFGRATEADLVKSLRANGKVVVS
                     LVAEEEGQVLGHVLFSPVVIEAVTARHAALGLAPLAVLPAFQRLGIGSALVSAGLARC
                     RALGHTRVVVLGEPAYYERFGFSTATDFGIRYPGAAPRAAFMALALVPGALDGRAGVA
                     RYAAEFEKL"
     gene            complement(3536..4543)
                     /locus_tag="VE030_07690"
     CDS             complement(3536..4543)
                     /locus_tag="VE030_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZF23331.1"
                     /translation="MEYRTLGFSGVKVSPLCLGTMMFGDRTEEKEAERIVGSALDAGV
                     NFIDTADQYAKGESERITGKLIAARRDYWVLATKVGNPMGDGPNERGVGRKWMMQAID
                     ASLKRLGTDYVDIYYLHLDYEDIPLEETVAAMAEILRTGKARYFGLSNFRGWRIAEVM
                     HLCRSMNVPRPVVCQPYYNAMNRQPEVEILPACHYYGIGVVPYSPLARGVLTGKYQPG
                     ETPPGDTRAGRGDKRMMETEFRKESLAIAQKIKIFAEKKGMSAGHFAVNWVLNNAIVS
                     SVIGGPRTLEQWQDYLKALEFSIDTKGEALVDSLVRPGHPSTPGFNDPRYPLSGRIVR
                     S"
     gene            4706..5689
                     /locus_tag="VE030_07695"
     CDS             4706..5689
                     /locus_tag="VE030_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HZF23332.1"
                     /translation="MRAIEITAPGGPEVLRVTRRPVPVPAVNEVLIEVVAAGINRPDV
                     LQRKGGYPPPPGASDIPGLEVAGTIVAAGAEVNGWRPGDRVCALVTGGGYAEYVAAPA
                     PQCLPIPRGLTLVEAAGLPETFFTVWVNVFERAGLKDSETLLIQGGSSGIGVTAIQMA
                     RAFGHRVFVTAGSADKCAACEKLGASRAINYRTEDFVAVVKALTGGKGVDVILDMVGG
                     DYVPRELACLADDGRLSLIAFLGGTRATIDMTDILRRRLTVTGSTLRARSVEVKGAIA
                     QVLKEKVWPLIDAGKIRPVIYRTFRLEEASAAHALMESSGHVGKIILEVRS"
     gene            complement(5686..6636)
                     /locus_tag="VE030_07700"
     CDS             complement(5686..6636)
                     /locus_tag="VE030_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23333.1"
                     /translation="MERRNDYDVTNQVNTTDPASVITEVNRIFLELYPNSSTHVLNRS
                     FRDLTRLHKGEYPGYRACDTGYHNLQHSLDVTLAMARLMDGYERSHDSHRTEGLGAKL
                     FRFGVVTALFHDIGYLRKVNDMRHKNGAEYTLKHVTRGGKFLEEYMSRLGMAELAPIA
                     SQIIHFTGYERPIRKIHVPNLTFRLLGNMLGTADIIAQMADRCYLEKCRDRLYPEFVE
                     GGLAARDDTDHRAGVMFKSAEDLVNKTPKFYETATVRLNDHLGSAYGYADKHFGGQNL
                     YLDEITKNIDYAVEVAEQHDLSLLRRTPPHLGEDGVDVNN"
     gene            6953..7150
                     /locus_tag="VE030_07705"
     CDS             6953..7150
                     /locus_tag="VE030_07705"
                     /inference="COORDINATES: protein motif:HMM:NF012755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HZF23334.1"
                     /translation="MSGLPREPRETLALSAPAPARGSYREYTVMTPRRKDRGARRIVA
                     VKRAEFHYTKDHYRSFRRIIE"
     gene            7147..7590
                     /locus_tag="VE030_07710"
     CDS             7147..7590
                     /locus_tag="VE030_07710"
                     /inference="COORDINATES: protein motif:HMM:NF013500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HZF23335.1"
                     /translation="MKTAFSKVVETGASGVYRCHSALAEVELGAAAQRGMRVEKVVLV
                     GARDKNAFLNAVAKALVFPDYFGHNWDAFYDCLVDLEHDKGEGLLVVLRDASGFARAE
                     PDEFAAAVDALQDAADFWEDEQKTLLVVVELEAAVLAPELPEVSG"
     gene            complement(7580..8110)
                     /locus_tag="VE030_07715"
     CDS             complement(7580..8110)
                     /locus_tag="VE030_07715"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HZF23336.1"
                     /translation="MIDRDGYRPNVGIVLCNGKNEVFWGKRVKEHAWQFPQGGIKSGE
                     TPEQAMYRELKEEVGLDPCHVKILGRTREWLRYEVPQTWVRRESRSVYRGQKQIWFLL
                     RLIGRDCDVCLRKSEHPEFDAWRWHEYWVPLESVIEFKREVYSRALQELARYLSSRAR
                     KEHAQRASRAASAINR"
     gene            complement(8191..8970)
                     /locus_tag="VE030_07720"
     CDS             complement(8191..8970)
                     /locus_tag="VE030_07720"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF003380.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HZF23337.1"
                     /translation="MFGKWKIHRSVDHEISVEVSEKDGIRSLHLGSETVQSSMKLADP
                     TELVLSYTRAMMAFLLFDPRPQNILMIGLGGGSLAKFVYRHLPSAQITVVESQLRVIA
                     AARQYFHVPSDDGRFRVELAEGSQWVAAHPRGCDVLLVDGYDGREQVPELCSESFYAD
                     ARAALTEGGILVVNLWSSDSRFDAYLQRIERVFDALACVPAERRGNVIVLAFARSPER
                     PRWDDLRLRARALQARYGLEFLQFVEGLRELNPHAEGRLTL"
     gene            9036..>10355
                     /locus_tag="VE030_07725"
     CDS             9036..>10355
                     /locus_tag="VE030_07725"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HZF23338.1"
                     /translation="MRTSQFFISTLKEAPSEAELISHKLMLRAGLIRKLGSGLYTWMP
                     LGLRVLRKVESIVREEMNRAGAVELLMPAIQPAELWQESGRWEQFGPQMLKIKDRHER
                     DFCFGPTHEEVITDIARKEIRSYRQLPLNFYQIQTKFRDEIRPRFGVMRAREFVMKDA
                     YSFHAGARDLAREYRNMYDTYSRIFSRLGLKFRAVAADTGAIGGTGSHEFHVLADSGE
                     DAIAYSPASDYAANVEFAEAVAPVKPRGAATQKMQKVPTPGKTTCEEVTAMLGLPLSQ
                     SVKALAVMHEERMLMLLVRGDHQLNETKVAKLPGLNPFRFATEKEIETRIGCRPGYIG
                     PVGLPKEITVIADRSVAAMKDFVCGANEAGYHLTGVNFGRDLEEPASVADIRNAVAGD
                     PSPDGKGLLEICRGIEVGHIFQLGSKYSKAMSATFLDESGKTQVMEMG"
BASE COUNT         1952 a         3302 c         3217 g         1884 t
ORIGIN      
        1 atggatgcag gtcgcggcgg gcatcatccc cgccgcggtg ccgcgcatcc tcggcgagga
       61 tcgggaaacc ggaatgttcg caatgcagtt cctggattcc gcgacttatc cggtgtggaa
      121 ggcacaactg cgcgacggcg aaatttccgc cgacaccgcg cgcgatgtcg gccaccgcat
      181 tgcggccatc cacggccgca ctgcgggcga ccccgccatg gcgctcacgt tcgccaccga
      241 tcacatcttc ttcccgatcc ggctcgaacc ctatcttgcc gccaccgcgg acaagcatcc
      301 cgactgcgcg gaacgcctgc gcagcctgat gcgggtgacc ggggccaccc ggaaagcact
      361 ggttcacggc gacgtcagcc cgaagaacat cctggccgga ccggatgggc cggttttcct
      421 cgacgccgag tgcgcatggt atggcgatcc cgccttcgat cttgcgttct gcctgaacca
      481 tctgctgctc aagtgcgtct ggcggccgca ttggcaggcg cgttacctgg tctgtttcga
      541 cgcgctcgcg caggcctatc tcgcagcggt gacgtgggag tcgcgcaccg acatcgaagc
      601 gcgcaccgcg cacctgctgc cgggactgtt tctcggtcga gtggacggca aatctccggt
      661 cgaatacatc gacagcgatg aagggcgcga acgcgtacgc cgggtcgcgc gcgcacttct
      721 gagcgcgccg gtcgacaaac tctccgacgt gcgggctgcc tggcagcacg aaatcgccac
      781 ttggaataag tgagccggac atgaccgata cgacaatcgc gagaatgcat ggcaggcggg
      841 tgtgggattc gcgcgggcgt ccgaccgtgg aagtggaagt tcacctcgca tccggcttca
      901 ccggacgcgc catcgcgccg gcgggcgctt cgcgcggctc caacgaggcg gtggatctgc
      961 gcgacggtgg cacggcattc ggcgggatgg gtgtcagcag ggcagtcgcg cacgtgaacg
     1021 gcgaaatttc ccgtctgctg atcggacgca atgcgctgca gcaggccgcg atcgacgagg
     1081 agctgatcgc gctggacggc actcccggca agtcacggct gggcggcaac gccatgatcg
     1141 ccacgtcgat ggcggtactg cacgccgcgg ccggcgccca cggcatgccg ctgtatgcct
     1201 acctcgccca agggaaaggc gtgcgcttgc cgctgccgga gatccagatc ttcggcggcg
     1261 gcgcgcatgc cggacggcgc gtggacattc aggatttcat ggtcatggca ctgggcgcac
     1321 gcagcttcga cgatgcggtc gcgatgacgg cggaggtcta ccgtgccgca ggcgaattga
     1381 tgaaggaagc gggaaagctg cagggggtcg ccgacgaggg cggtttctgg cccgcattcg
     1441 cctcgaacga agaggcgctg accatgttga tgcgtgcgat cgagcgcgcc ggttaccacc
     1501 ccgacgaaga catcggcatc tcgctcgatg tcgccgcctc cgagttaggc cgcaacggac
     1561 gttaccggct cggcctggag tcccgcgaac tcgacagcga cgatttgtgc gaattgctgc
     1621 tcggctggct tgcgcgctac ccgatcgttt caatcgagga tccgcttgcc gaagatgacg
     1681 ccgaaggcat gaagaaattc accgctgctg caggaagccg ggcccagatc atcggcgatg
     1741 attttctggt caccaacgcc gcgcgggtga agaacgcagc cgccgctgga gcctgcaatg
     1801 cagtgctgat caagcccaat caggcgggca ccgtcaccga gaccaaggcc gcgctcgatg
     1861 cgggcaagcg cgccggctgg ggcaccatcg tctccgcccg ctcgggcgag accgaggacg
     1921 tcaccatcgt ccacctcgcg gttggctggg atgccggcca gctcaaggtg ggatcgttcg
     1981 cgcgctccga acggatggcc aagtggaacg aggtactgcg aatcgaggag gcgctaggtg
     2041 ccaacgcggt cttcgccgga cgagcggtat tgccgcgact ggcttgaacc acagagacac
     2101 agaggcacgg agaattccgg aacgaaaacc ttgatacgga aaaatttggt gcagcaaact
     2161 cgctacgagt tacttctgat ttcaatcaag tttgcggttc tccgtgcctc tgtgtctctg
     2221 tggtgaagaa ttgcttgatc tacaatgacc acagaccacg tccaccatcg ccgcactgcg
     2281 cgcggcattc tgctgatcct ggcggcggtg ttcatgttct ccggcatggt cacgctcgga
     2341 ttcatgcgac gattgagcga tgccagcgac caatccggaa cccactgaaa cccgaaaacc
     2401 catgaacaga ccggcgcccg agaaccgcaa ctcctatcgc catttccagt cgatccccac
     2461 ccgatggatg gataacgacg tttacggcca cgtcaacaac gtcgtctact acagctactt
     2521 cgacacggtg gtgaaccagt acctgatcga gcagcgcgcc ctgaacatcg acaccagcaa
     2581 ggtcatcggg ctggtagtgg aaacgcaatg ccggtacttc gcctccatca ccttccccga
     2641 tgtggtgcac gccggactga gggtggcgac actgggtaac agcagcgtac gctacgagat
     2701 cgggctattc cgcaacgacg aacagaccgc gtcggcccag ggctacttca tccacgtcta
     2761 tgtggaccgc gccactcgtc gcgctgtttc tttgcccgcc gacctgcgcg ccgcgctgga
     2821 aagaatcccg atccaatcca aaccctgagc cacagaggac acagagagca cagagaaaag
     2881 cgaagaacgc gaacaaaaat gaaaagcggc acaatcattc gcgcatgcgg tagcgaacgt
     2941 acgtgttttc tttttatggc tcagattttc tgctctttcc tctgtgctct gtgtcctctg
     3001 tggcgaatga gttttcacag cttttcaaat tcggcagcat agcgcgcgac gcctgcgcgc
     3061 ccgtcgagcg caccgggcac cagtgccagg gccatgaagg ctgcgcgcgg cgcggcgccg
     3121 ggatagcgga tgccgaagtc cgttgccgtg gaaaacccga atcgctcata gtaggccggc
     3181 tcgccgagca cgaccacacg ggtatggcca agcgcgcggc agcgggccag cccggcgctg
     3241 accaacgcgc tgccgatgcc caggcgctgg aatgccggca acaccgcgag cggcgccagt
     3301 cccaatgccg catgcctggc cgtgaccgct tcgatgacga ccgggctgaa caacacgtgc
     3361 ccgagcacct ggccctcttc ctctgccacc agcgatacca ctaccttgcc attcgcgcgc
     3421 aggcttttca ccagatcggc ttcggttgcg cggccgaatg cctgcgcaac gaccgcatga
     3481 atcgccgcct catcgcgcgg ccgctcgtcg cggatgtgcg gcatcaagct ttcggctagc
     3541 tgcggacgat gcggccactg agcggatagc gcgggtcgtt gaagcccggc gtggatggat
     3601 gcccggggcg caccagcgaa tccaccagcg cctctccctt ggtatcgata ctgaattcga
     3661 gcgccttcag atagtcctgc cactgctcca gcgtgcgcgg gccgccgatg acactgctga
     3721 cgatcgcgtt gttgagcacc cagttgaccg cgaaatggcc ggcgctcatc cccttcttct
     3781 ccgcgaaaat cttgatcttc tgcgcgatcg ccagcgactc cttgcggaat tcggtctcca
     3841 tcatgcgctt gtcgccgcgg ccggcacggg tatccccggg gggcgtttca ccgggctgat
     3901 acttgccggt gagcacgccg cgcgccagcg ggctgtaggg caccacgccg atgccgtagt
     3961 aatggcaggc cggcaggatt tccacttcgg gttggcggtt catcgcattg taatatggct
     4021 ggcacaccac cggcctgggg acattcatcg agcggcacag gtgcatcacc tcggcgatgc
     4081 gccagccgcg gaaattcgaa agaccgaagt aacgcgcctt gcccgtgcgc aggatctccg
     4141 ccatcgccgc caccgtctct tccagcggga tgtcctcgta gtcgaggtgc aggtaataga
     4201 tgtccacgta atcggtgccg aggcgcttca ggctggcgtc gatcgcctgc atcatccatt
     4261 tgcggccgac tccgcgctcg ttcggcccgt cccccatcgg attgccgacc ttggtggcga
     4321 gcacccagta atcgcgacgg gcggcgatca gtttcccggt gatgcgctcc gactcgccct
     4381 tggcgtactg atcggcggtg tcgatgaaat tgaccccggc gtcgagcgcc gagccgacga
     4441 tgcgctcggc ttccttttcc tcggtgcggt ccccgaacat catggtaccg aggcaaagcg
     4501 gcgagacctt cacgccgcta aagccaagcg tgcgatattc cattgcgtcc tcccgcggaa
     4561 tgtttgggtg aaatgcggcg atgatgccgc aagcccaccg ccggcgcaaa cgcttgaggc
     4621 agccgttgcg ccaatgctat aagagttctt cgccacaacc gtcgacaatc ttcagcccat
     4681 catgcctgca acgttctctg cgcaaatgcg cgcaatcgaa atcaccgcgc cgggcggacc
     4741 cgaggtcctg cgagtcaccc ggcgtccggt ccccgttccc gcagtcaatg aagtgctgat
     4801 cgaggtcgtc gcggccggca tcaaccgtcc cgacgtcctg cagcgcaagg gcggctaccc
     4861 gcccccgccc ggcgcatccg acatccccgg gctcgaggtc gccggcacga tcgtcgcagc
     4921 cggcgcggaa gtgaatggat ggagacccgg tgaccgggtc tgcgcgctgg tgacgggcgg
     4981 gggctacgcc gagtatgtcg ccgccccggc gccgcagtgc ttgccgatcc cgcgcggcct
     5041 cacgctcgtc gaagccgcgg ggcttccgga gacctttttc actgtgtggg tcaatgtgtt
     5101 cgagcgggcg gggctgaaag attcggagac gctgctgata caaggaggat cgagcggcat
     5161 cggcgtgacg gcgatccaga tggcgcgggc attcggccat cgcgtgttcg taaccgcggg
     5221 ttcggccgac aagtgtgctg cctgcgagaa gctcggcgcg agccgcgcga tcaactaccg
     5281 gacggaggat ttcgtggcgg tggtgaaggc actgaccggc ggcaagggcg tggacgtgat
     5341 cctggacatg gtgggcggcg attacgtgcc gcgtgaactg gcctgcctcg ccgacgacgg
     5401 gcggctgtcg ctgatcgcct tcctcggcgg caccagagcc acgatcgaca tgaccgacat
     5461 cctgcgccgg cgcctgaccg tcaccggttc gacgctgcgc gcccgctccg tcgaagtcaa
     5521 aggagcgata gcgcaggtgt tgaaggagaa agtctggccg ctgatcgacg ccggaaaaat
     5581 caggccggtg atctaccgga ccttcaggct ggaggaggcc tctgcagcgc acgcgctcat
     5641 ggaatcgagc gggcacgtcg gcaagattat cctcgaagtg aggtcctaat tattgacgtc
     5701 gacgccatct tcgccaaggt gcggcggcgt gcgccgcaac agtgacagat cgtgctgctc
     5761 ggcgacttcc acggcgtagt cgatattctt ggtgatttcg tccagataca gattctggcc
     5821 gccgaaatgc ttgtcggcgt agccgtaagc gctgccgaga tgatcgttca gtcttaccgt
     5881 cgccgtctcg tagaatttcg gcgtcttgtt gaccaggtct tcggccgact tgaacatcac
     5941 gccggcgcgg tggtctgtgt catcgcgcgc cgccagcccg ccttcgacga actccggata
     6001 aagccggtcg cggcatttct ccagatagca gcgatccgcc atctgcgcaa tgatgtccgc
     6061 ggtgccgagc atgttgccca gcagccggaa agtgagattc gggacgtgga ttttccggat
     6121 cggcctttcg tagccggtga agtgaatgat ctgcgaggcg attggcgcga gctccgccat
     6181 ccccagcctg ctcatgtact cttccaggaa cttgccgccg cgggtgacgt gcttgagcgt
     6241 gtattcagcg ccgttcttgt ggcgcatgtc gttgaccttg cgcaagtagc cgatgtcgtg
     6301 aaacagcgcc gtcaccaccc cgaaacggaa caacttggca ccgaggccct cggtgcgatg
     6361 actgtcgtgc gaacgctcgt atccgtccat cagccgcgcc atggcaagcg tgacgtcaag
     6421 cgagtgctgg agattgtgat agccggtatc gcaggcccgg taacccggat actcgccctt
     6481 atgcaggcgc gtgagatccc tgaacgaccg gttcaggacg tgggtggaag agttcggata
     6541 gagctccagg aagatacgat tgacttcggt gatcaccgac gccgggtccg tggtgttgac
     6601 ctggttggtc acatcgtagt cattccgccg ttccatttag cccttgtccg gtgaattccg
     6661 cccgcatgac caacacatgg taacctcgcc ccctcggccc aatcaaggcg cttattctcc
     6721 ccgcaaggcg ggcaccgcgc cggtcctcct gcgaccgatt tcaaaaagct cgtggtttct
     6781 ggtaatcacc tgaatatccg tgagaatatt cccggccgag gcaggacgcc aataagttct
     6841 cggcgtctag ctctggttct gggcagactc ctgattctcg gctgcgctcg cagctctggc
     6901 cgcgcagccg cacgaccgcg aagcacccta tcccgccact cccggaatcc gggtgtccgg
     6961 tctgccgcgc gagccacgcg agacgctggc cttatccgcg ccggcgcccg cgcgtggcag
     7021 ctatcgcgag tacaccgtca tgacaccaag gaggaaggat cgtggcgccc gcagaattgt
     7081 tgccgtcaag cgcgccgagt tccactacac taaagatcat taccgcagct tcagacgcat
     7141 catcgaatga aaaccgcctt ttccaaagtg gtcgaaaccg gcgcgagcgg cgtgtatcgc
     7201 tgccactcgg cgttggcgga agtggagctg ggcgcagccg cgcagcgcgg catgcgcgtg
     7261 gaaaaggtgg tgcttgtggg ggcgcgcgac aagaatgcat tcctgaacgc ggttgccaag
     7321 gcgctggtgt tcccggacta cttcggccac aactgggacg ccttttacga ttgcctcgtg
     7381 gacctggaac acgacaaggg cgagggattg ctggtagtgc tgcgcgacgc gtccgggttt
     7441 gcccgcgccg aaccggacga gttcgccgct gcggtggatg cgctgcagga cgccgcggac
     7501 ttctgggagg acgagcagaa aacgctgctg gtggtggtcg agctggaggc agccgtgctg
     7561 gcgcccgagt tgcccgaagt cagcggttga tagcgctggc cgcccgcgat gcacgctgtg
     7621 cgtgctcttt tcgcgcgcgg gaactgagat aacgggcgag ttcctgcagc gcgcggctgt
     7681 atacctcgcg cttgaactcg atcacgcttt ccagcgggac ccagtactcg tgccagcgcc
     7741 aggcatcgaa ctccggatgc tcggatttgc gcagacacac gtcgcaatcg cgcccgatca
     7801 aacgcagcag gaaccagatc tgtttttggc cgcgatacac gctgcggctc tcgcgccgca
     7861 cccaggtctg cggcacctcg taacgaagcc actcgcgggt acggccgaga atcttcacat
     7921 ggcagggatc gaggccgacc tcttccttca actcccggta catcgcctgc tccggtgttt
     7981 caccggactt gatgccgccc tgtggaaact gccaggcgtg ctccttgacc cgcttgcccc
     8041 agaagacctc gtttttgccg ttgcacagaa caattcctac attcgggcga tagccgtcac
     8101 ggtcgatcat gtcagccacc ccaatcacca atccagttga ttggattctt ccataaatac
     8161 agcaacgaag gaaggcgctg gcgatggtgg tcagagcgtg aggcggcctt ccgcatgggg
     8221 gttaagttcg cgtaaaccct cgacaaactg caggaattca aggccgtacc gggcttggag
     8281 ggcgcgcgcc ctgagccgca aatcgtccca acgcgggcgt tccggggagc gcgcaaaggc
     8341 cagcacaatg acgttgccgc gcctttccgc tggcacgcac gccagggcat cgaatacccg
     8401 ctcgatgcgc tgcaggtagg catcgaagcg gctatccgaa ctccacaggt tcaccaccag
     8461 tattccgcct tcggtcagcg ccgcgcgcgc atcggcgtaa aaactctccg agcaaagctc
     8521 gggaacctgt tcgcggccgt cgtatccgtc caccagcaac acgtcgcaac cgcgcggatg
     8581 agcggccacc cattgactac cttcggccag ttcgacgcga aaccggccat cgtccgacgg
     8641 cacgtggaaa tactggcgcg ctgccgcgat cacccgcagt tgcgattcga ccacggtgat
     8701 ttgcgccgac ggcaggtggc gatagacgaa cttggccagc gacccgccgc ccaagccgat
     8761 catcagaatg ttttgcgggc gcggatcgaa cagcaggaac gccatcatgg ccctggtata
     8821 cgacagcacc agctcggtgg gatcggccag cttcatcgaa ctctgcaccg tctcactgcc
     8881 caggtgcagc gagcgaatgc cgtccttctc gcttacttcg acgctgattt catgatcgac
     8941 cgaacgatga atcttccatt ttccgaacac cgctgctctc cacttcggga tggctctcta
     9001 ccgtagaata gcggcttttc gagctcgggc aagacatgcg cacttcgcag ttcttcattt
     9061 ccacgctcaa ggaagctccc agcgaagcgg aactcatcag ccacaaactg atgctcaggg
     9121 cggggctgat caggaaactc ggcagcggcc tgtatacctg gatgccgctc ggtctgcggg
     9181 tgctgcgcaa ggtggagagc atcgtgcgcg aggagatgaa ccgcgccggc gccgtggagc
     9241 tgctgatgcc ggcgatccag ccagccgaac tgtggcagga gtccggccgc tgggaacagt
     9301 tcggcccgca gatgctcaag atcaaggatc gccacgaacg cgacttctgc ttcggcccga
     9361 cccatgagga agtcatcacc gacatcgcgc gcaaggagat ccgctcctac cgccagctgc
     9421 cgctcaattt ctaccagatc cagaccaagt tccgcgacga aatccggccg cgtttcggcg
     9481 tgatgcgcgc acgcgaattc gtgatgaagg acgcctactc ctttcacgcc ggcgcccggg
     9541 atctggctcg cgaataccgc aacatgtacg acacttattc gcgcatcttc tcccgtctgg
     9601 ggctgaagtt ccgcgcggtc gccgccgaca ctggcgccat cggcggcacc ggctcgcacg
     9661 agtttcacgt gcttgccgac tccggcgagg acgcgatcgc ctactccccc gcctcggatt
     9721 acgccgccaa cgtcgagttc gcggaagcgg ttgcgccggt gaagccgcgc ggcgcggcca
     9781 cgcagaaaat gcagaaggtt cccacgcccg gcaagaccac ctgtgaggaa gtcaccgcga
     9841 tgcttggact gccgctgtcg cagtcggtca aggcgctcgc ggtcatgcat gaagagcgga
     9901 tgctcatgtt gctggtgcgt ggcgaccacc agctgaatga aacgaaggtg gcgaagcttc
     9961 ccggcctgaa tccgttccgt ttcgccaccg aaaaggagat cgaaacacgc atcggctgcc
    10021 gaccgggcta catcggtccg gttggcttgc cgaaggaaat taccgtgatt gcggatcgct
    10081 cggtcgcggc gatgaaagat ttcgtctgcg gcgccaatga ggcaggttat cacctgaccg
    10141 gcgtgaactt cggccgtgac ctcgaggaac cagcatcggt cgccgacatc cgcaatgccg
    10201 tggcaggcga cccgagccct gacggcaagg gcttgctgga gatctgccgc ggcatcgagg
    10261 tcggacacat tttccagttg ggcagcaaat actccaaggc gatgagcgct accttcctgg
    10321 acgagtccgg caagacacag gtaatggaaa tgggc
//
LOCUS       DASQOP010000186         6115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_276712, whole genome shotgun sequence.
ACCESSION   DASQOP010000186 DASQOP010000000
VERSION     DASQOP010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6115
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276712"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VE030_07730"
     CDS             complement(<1..313)
                     /locus_tag="VE030_07730"
                     /inference="COORDINATES: protein motif:HMM:NF022592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HZF23339.1"
                     /translation="MHSVMISWNAWRVNGAAGPQLEVSTMQIEINRSTLSLADEGLVA
                     IRDGQGTRIVCHAGSLWITQECDVKDSVISAGESFTVRNPGLTLLTALSASELTIIEP
                     RA"
     gene            375..1346
                     /gene="gcvA"
                     /locus_tag="VE030_07735"
     CDS             375..1346
                     /gene="gcvA"
                     /locus_tag="VE030_07735"
                     /inference="COORDINATES: protein motif:HMM:NF008352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HZF23340.1"
                     /translation="MANRVPALPSLDFFKGFEAAARHLSFTKAAEELFVTQSAVSRQI
                     QALEQRLGVTLFLRRNRGLALTEAGEQLRRAVDSALRILHQAVEQVSPGATPKMVTVT
                     SSIAFSSLWLIPRLSGFRRLHPDVDVRISANNQVLDLDRERIDLAIRYCPSRAAPAGS
                     VRLFGEEILPVCSPALLRDRNRPLKSPQDLRHHVLLHYDEPQRPVPWLTWNVWLETAG
                     VPDLEPADSLRFNHYDQTIGAALGGQGVALGRRPLVKKLLADGALVAPFPLEAVTGRA
                     YFIVRTPITSGRPEVTHFAEWLIAEAAAMTARESDGVRKPRGGKKVD"
     gene            1417..1728
                     /locus_tag="VE030_07740"
     CDS             1417..1728
                     /locus_tag="VE030_07740"
                     /inference="COORDINATES: protein motif:HMM:NF025691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HZF23341.1"
                     /translation="MENPLQQMNDSSRWARLLYMILFAVLYKVAEFIMWVVVLFQVVV
                     TLVTGSPNERALKFGKQLSVYVYGLWAYLTYNTEKKPFPFSDWPTTTAPPSPPQGDAR
                     A"
     gene            complement(1843..2946)
                     /locus_tag="VE030_07745"
     CDS             complement(1843..2946)
                     /locus_tag="VE030_07745"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HZF23342.1"
                     /translation="MDEKLVYNVNVTAQDVLPTPEEIKRRVPLTDKAEMTVLEGRHAL
                     ERILDRMDHRLMVVIGPCSIHDPVAAMDYARRLKGLADEVGDTLFIVMRVYFEKPRTT
                     TGWKGLINDPRMNDSFHIEEGLQLARKVLRDINELGLPAGTEALDPISPQYLGDLVTW
                     SAIGARTTESQTHREMASGLSTPVGFKNGTDGGLQVAINALLSVSRPHSFLGINQSGQ
                     VSVIRTKGNGYGHIVLRGGAKGPNYDSVTIAVVEKELAKNKLSANIAVDCSHANSNKD
                     HNLQPLVASDCAHQIMEGNRSIVGLMLESNIKAGNQSIPADLKQLKYGVSVTDACIDW
                     PTTEKLLREIRQKLKDVLPKRVPRTAEIRKISG"
     gene            complement(3180..3506)
                     /locus_tag="VE030_07750"
     CDS             complement(3180..3506)
                     /locus_tag="VE030_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007732618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HZF23343.1"
                     /translation="MIYELRIYHACPGKLPALNARFSNHTLKIWERFGIRQVGFWTTA
                     IGESNNDLYYILEWENLAEREQKWNAFVTHPDWLAIRAETEKDGQLVNHFTNMILTPT
                     AYSKMR"
     gene            complement(3615..4184)
                     /locus_tag="VE030_07755"
     CDS             complement(3615..4184)
                     /locus_tag="VE030_07755"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HZF23344.1"
                     /translation="MLALFEGKALLAVDGGKPRTVRAGESYAGVKLISANSEEAIVSV
                     NGKRQRLRIGEGVYSAVSPQRERTTVTLTTDGGGHFVTTGSINGATMNFLVDTGATAV
                     SMNLNDARRAGVNYLAGAKAYSQTANGVVPIYKVKLGSVKLGDITLTDVDGFVHQSDA
                     LPVVLLGMSFLGRLEMRREGESLTLTKKQ"
     gene            4279..4986
                     /locus_tag="VE030_07760"
     CDS             4279..4986
                     /locus_tag="VE030_07760"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HZF23345.1"
                     /translation="MKPVAIFRHSPTEGPGYFATYLESRNIPWHLIAVDQGNAVPPDP
                     QQFGGLVFMGGPMSVNDELPWIAPVLQLIRQAAALDVPVLGHCLGGQLISKAFGGTVA
                     RNPVKEIGWGEVLIADNPVARQWFGPGIERFESFHWHGETFTIPQGAVRIMGNRYCEN
                     QGFVIGNSLGMQCHVEMTEELVKSWCETGADEVLNSAGPAVQSPQRMQRGMQEKLAAL
                     RSVADRLYDQWVKGLKA"
     gene            4992..5870
                     /locus_tag="VE030_07765"
     CDS             4992..5870
                     /locus_tag="VE030_07765"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZF23346.1"
                     /translation="MALVEVYRSDRRADCDERALVLAAVGVASELVSLGDSFALLVPE
                     DSVTAAMTHLKDYDEEGRSRQREPGPIRAHPDAWLGSAGYAVVLVTVADCAARGLLGF
                     DWFSSGELVSGLTRSGELWRVVTALTLHVDSGHLITNLGFGMLFGYLAGQLLGPGIAW
                     ASILAAGALGNLFDALLMPPTHRAIGASTAVFATLGLLAAYSWRRRSGRSARWPYRWA
                     PLIAAVALLALIGTGGEHTDVLAHLTGFFAGTVFGVVHGSLAVPGSGRALTQLLAGAS
                     TLAVIVGAWLLALIRA"
     gene            complement(6027..>6115)
                     /locus_tag="VE030_07770"
     CDS             complement(6027..>6115)
                     /locus_tag="VE030_07770"
                     /inference="COORDINATES: protein motif:HMM:NF016355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF520 family protein"
                     /protein_id="HZF23347.1"
                     /translation="KRDDLQQVITMMKKNVTDYPLQFANFRD"
BASE COUNT         1033 a         1848 c         1930 g         1304 t
ORIGIN      
        1 cagcgcgcgg ctcgatgatg gtgagttcgg aagcgctcag cgcggtcagc agcgtcaacc
       61 cgggattgcg tacggtgaac gattcgccgg ccgaaatgac ggagtctttc acgtcgcact
      121 cctgcgtgat ccacaggctg cccgcgtggc agacgatgcg ggtgccctgg ccgtcgcgga
      181 ttgccaccag cccctcatcg gcgaggctca gcgtggaacg attgatctcg atctgcatgg
      241 ttgacacctc cagttgtggc ccggcggcgc cattgacgcg ccaggcattc caggaaatca
      301 ttacggaatg cattttccgc agtgcacagc agggcacaaa cgatatttca gaatgcttcg
      361 catgagttgc gagaatggct aacagagtgc ctgcgctgcc gtccctggac ttcttcaagg
      421 gcttcgaggc tgccgcccgg cacttgagct tcaccaaggc cgccgaggaa ctgttcgtca
      481 cccaatcagc ggtcagccgc cagatccagg cgctggagca gcggctgggc gtgacgctgt
      541 tcctgcggcg aaatcgcggg ctggccctca ccgaggcggg cgagcagttg cggcgcgccg
      601 tcgacagcgc gctgcgcata ctgcatcagg cggtcgagca ggtttcgccg ggtgctacgc
      661 cgaaaatggt cacggtgacc tcatcgatcg ccttcagttc gctgtggctg attccgcggc
      721 tgtccggctt tcgccggctg catccggacg tggacgtgcg catctccgcc aacaaccagg
      781 tactcgacct cgatcgcgag cgcatcgatc tcgctatccg ttattgtccg tcgcgtgccg
      841 cgcctgccgg atcggtgcgt ctgttcggcg aggagatcct gccggtgtgc agtcccgcgc
      901 tgctgaggga tcggaaccgg ccgctgaaat caccgcagga cctgcggcac cacgtgctgt
      961 tgcactacga cgaaccgcag cgcccggtac catggctcac ttggaacgtc tggctcgaga
     1021 ccgcgggcgt gcccgacctc gagccggcgg attctctgcg cttcaatcac tatgaccaga
     1081 cgataggcgc ggcgctgggc gggcagggcg tggcgctggg ccgcaggccg ctggtgaaga
     1141 aactcctggc cgacggggcg ctggttgcgc catttccgct cgaagcggta accggccggg
     1201 cctacttcat cgtcagaacc ccgatcacgt cgggtcgtcc ggaagtgacc catttcgcgg
     1261 agtggctgat cgccgaagcc gcggccatga ccgccaggga aagcgacggc gtgcgcaaac
     1321 cccgtggcgg gaaaaaagta gactgagttt cccggtgggg atcccatcta ccacaggggt
     1381 ccggtttcag cagccgcggg tggcaaagga gaagcaatgg aaaatccgct acagcagatg
     1441 aacgattctt cgcgatgggc gcggttgttg tacatgatcc tgtttgcggt tctgtacaaa
     1501 gtggccgaat tcatcatgtg ggtcgtggtc ctgttccagg tcgtcgttac cctggtaacg
     1561 ggcagtccca acgaacgcgc gctgaaattc ggcaagcagc tttcggttta tgtctatggc
     1621 ctgtgggcct acctgaccta caacaccgag aagaagccct ttccgttctc ggactggccg
     1681 accaccaccg cgccgccgtc tcctcctcag ggcgatgcgc gagcgtaact gcagggctca
     1741 gagcgatggc cgtctgtcct aaatgcaaac gggcgaccag ttggtcgccc gctgtcaatc
     1801 cgttgacttc tccgcccgat tgcggagatc gaccgacggt agtcagccgc tgatctttct
     1861 gatttccgcg gttctcggaa cccgttttgg caacacgtcc ttcagcttct gccggatttc
     1921 gcgcagcagc ttttccgtcg tcggccagtc gatgcaggca tcggtgacgg acaccccgta
     1981 cttgagctgt ttcaggtcgg ccgggatcga ctggttgccg gctttgatat tgctctccag
     2041 catcaagccc acgatcgacc ggttgccttc catgatctgg tgcgcgcagt cgctggccac
     2101 cagcggctgc aggttgtggt ctttgttgga gttggcgtgg ctgcagtcga ccgcgatgtt
     2161 ggcggacagt ttgtttttcg ccagctcctt ttccaccacc gcgatcgtca ccgaatcgta
     2221 attcgggccc ttggcgccgc cgcgcaggac gatgtggccg tagccgttgc ccttggtgcg
     2281 aatcaccgaa acctggccgc tctggttgat gccgaggaaa ctgtgcggcc gcgataccga
     2341 caacagcgcg ttgatcgcca cctgcagccc gccgtcggtg ccgttcttga agccgaccgg
     2401 cgttgacagg ccgctggcca tctcgcggtg cgtctgtgat tccgtggtgc gcgcgccgat
     2461 tgcactccag gtcaccaggt cgccgagata ctgcgggctg atcggatcga gcgcctcggt
     2521 gccggcggga agtcccagtt cgttgatgtc gcgcagcacc ttgcgggcca gctgcaggcc
     2581 ttcctcgata tggaaagagt cgttcatgcg cgggtcattg atcaggccct tccagcccgt
     2641 ggtggtgcgc ggcttctcga aatagacgcg catcacgatg aacagcgtat cgcccacttc
     2701 atcggcgagc cccttcagcc gacgggcgta atccatcgcc gccaccgggt cgtggatcga
     2761 gcacgggccg atcactacca tcagccggtg atccatgcgg tcgagaatgc gttctaaggc
     2821 gtgccggcct tccaggaccg tcatttccgc tttgtcggtc aagggcacgc ggcgcttgat
     2881 ttcctccggg gtggggagga cgtcctgggc cgtgacgttg acgttgtata cgagtttctc
     2941 gtccatgatg agcgcctgcg ctgcctgaaa acacggaccg cgttgtgcga ccctgtcgga
     3001 gcattctact taaagcccgg aggccgtcaa agccgtcgtt ccggggacga cctcaccacc
     3061 tcatctcccg ccctctccac agagcagaga aggtacgctc cgcgggcgta tggcgctttt
     3121 gaccatcaat ttccctctcc cctcgcggga gagggtcagg gagaggggga ggggtggctc
     3181 tagcgcattt tcgagtatgc agtcggtgtc aggatcatgt tggtgaagtg gttcaccagc
     3241 tggccgtcct tttctgtttc ggcacggatc gccagccagt cgggatgggt gacgaaggcg
     3301 ttccacttct gctcgcgctc ggccagattc tcccattcga ggatgtagta caggtcgttg
     3361 ttgctttcgc cgatggccgt tgtccagaaa cccacctggc ggatgccgaa gcgctcccat
     3421 attttcaggg tatggttgga aaagcgtgcg ttcagggccg gcagtttgcc ggggcaggcg
     3481 tggtaaatgc gcagttcgta gatcattgct cctccgcggt tgtttcaaat ttttcgttgt
     3541 ggggatgctg ggatgacggg tgcccggcgc gccatcggtt tccggcgccg gcgcaatgtg
     3601 caaaggtgtc agtattattg tttcttggtg agcgtaagac tctcgccctc ccggcgcatt
     3661 tccaggcgcc cgaggaaact cattccgagc agcacgacgg gcagcgcgtc gctttgatgc
     3721 acgaaaccgt cgacgtcggt cagggtgata tcccccagtt ttacgctgcc gagttttact
     3781 ttgtagatcg gcacgacgcc gttcgcggtc tgcgagtaag ctttcgctcc ggccaggtag
     3841 ttgacgcccg cccgtcgcgc atcgttcagg ttcatcgaca ccgccgtggc gccggtgtcg
     3901 accaggaaat tcattgttgc gccgttaatg ctcccggtgg tgacgaaatg tccgcccccg
     3961 tccgtagtaa gcgtgaccgt tgtacgttcc ctttgcgggg ataccgcgct gtaaacaccc
     4021 tcgccgatcc tgagccgctg acgtttgccg ttgacggaga cgatggcttc ctcgctgttg
     4081 gcgctgatga gcttgacgcc ggcgtaggat tccccggcac gtacggtgcg cggcttgccg
     4141 ccgtccaccg ccagcaaggc tttgccttcg aacagtgcga gcacgctcac ttcctgcgcg
     4201 gctgccgggc aggttaggag ggcaacaata attgcagtaa cattcgcggc cttgaaccgc
     4261 atgaggatcc ctttggcgat gaaacctgtt gcaatattcc gccactcgcc cacggaagga
     4321 ccaggttatt ttgccacgta tctggaatcg cggaacatcc cgtggcacct gatagccgtg
     4381 gaccagggca atgcggttcc gcccgacccg cagcaattcg gcggtctggt gttcatgggc
     4441 gggccgatga gcgtgaacga cgaattgccc tggatcgcgc cggtgttgca gctcatccga
     4501 caggcggccg cgctcgatgt tcctgtcctc ggtcattgcc tcggcggaca gttgatttcg
     4561 aaggcattcg gtggcacggt cgcccgcaat ccggtcaagg agatcggctg gggcgaagtc
     4621 ctgattgcgg acaatccggt cgcgaggcag tggttcggac ccggcatcga gcgcttcgaa
     4681 tcgtttcact ggcacggcga aacgttcacc attccgcagg gcgcggtgcg catcatgggc
     4741 aaccgctact gcgagaacca gggcttcgtg atcggcaaca gcctgggcat gcagtgccac
     4801 gtcgaaatga ccgaggaact ggtcaaaagc tggtgcgaga ccggggcgga cgaggtctta
     4861 aacagcgccg gtccggcggt gcaatcgccg cagcggatgc agcgtggaat gcaagagaag
     4921 ctggcggccc tgcgcagcgt ggcggaccgg ctctacgatc aatgggtcaa gggactgaag
     4981 gcgtaacagg cgtggcgctc gtcgaagtct atcgatccga tcgtcgcgct gactgcgacg
     5041 aacgcgcctt agtccttgcc gccgtcggtg tggcatcgga gcttgtatcc ttgggcgaca
     5101 gcttcgcact gcttgtgcca gaggactcgg tcaccgcggc gatgactcac ctcaaggact
     5161 atgacgagga gggccgctct cgccaacggg agcccggccc gatcagggcg caccccgacg
     5221 cgtggcttgg ttcagcagga tacgcagtgg tcctcgtaac cgtcgcagac tgcgccgcac
     5281 gcggtcttct gggcttcgat tggttttcgt cgggtgagct tgtctcggga cttacaagaa
     5341 gcggggagtt gtggcgcgtg gtcaccgcgc ttacgctgca cgttgactcc ggccacctga
     5401 tcacaaattt aggcttcggc atgttgttcg gttatctcgc gggccaattg ctcgggccgg
     5461 ggatcgcctg ggcgagcatt ctcgcggcgg gtgcacttgg gaatttgttt gacgcgctgc
     5521 tcatgccgcc aacccatcgt gcaatcggcg catcgactgc agtattcgcg acgcttggtc
     5581 ttcttgccgc gtactcctgg cgtcggcgct ccggacgcag tgcacgctgg ccctatcggt
     5641 gggcgccatt gattgccgcc gttgctctgc ttgcgctcat cggtaccgga ggggagcaca
     5701 cggatgtgct cgcgcatctc acgggattct ttgccggcac ggtgttcggt gttgtacatg
     5761 gaagcctggc cgtacccgga tccggtcgcg ccctgacgca actgctggcc ggcgcgtcga
     5821 cgttggcggt aattgttggc gcatggctgt tggccctgat ccgcgcgtag cggcaccgcg
     5881 tatcagctca agcctgagct actacacgtt attcacaaag gtagaggtac cctacagggc
     5941 tgacttcgga ccccgcttcg atcctcattc acagaattct ttgcgtcttt ctttttcctt
     6001 tgcgaccttt gcgtcgaacg ccttaatcag tcgcggaaat tggcgaactg cagcggatag
     6061 tccgtcacgt tcttcttcat catcgtgatc acctgctgaa gatcgtcacg cttgg
//
LOCUS       DASQOP010000187         8632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1487991, whole genome shotgun sequence.
ACCESSION   DASQOP010000187 DASQOP010000000
VERSION     DASQOP010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8632
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487991"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..618
                     /locus_tag="VE030_07775"
     CDS             172..618
                     /locus_tag="VE030_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23348.1"
                     /translation="MFDRETMEETTAFLDVVTRESGIHGRSNILIYVHSSAPIFHVEY
                     HGFIDCFKEIAKKPGHQIALLADSKDLYISHEYLELRARQYGLNVRNFRNEAAALEWL
                     ANRRRAPDRRGQQERRWRQEPLKHQERRLHPERRQRMRRGGLESPL"
     gene            858..2237
                     /locus_tag="VE030_07780"
     CDS             858..2237
                     /locus_tag="VE030_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23349.1"
                     /translation="MERRVRGRLAVGPEGVLRAPVVAACLVILSACNASPSQDLVAKG
                     RQIFFNETFQGNGRTCGTCHPAENNLTLDPAFIATLAPNDPLFVAEFNPDLRKNFENP
                     RLMRQFGLILENLDGFVDLDKKFVMRGVPHMLGLRTSVDSPQGPRTGWSGDGAPGDGS
                     LRSFATGAVIQHFTKTLNRIPGQDFRLPTEEELDAIEAFSLSLGRQQDLALPLPLKGI
                     VPKRGQEIFLDNSLGKCNRCHANAGATANFGAGSIGNANFNTGVENLPDQPADLSGEL
                     NPADDGFRTPGDGTFNTPPLVEAAETGPFFHNNAIETIEGAVAFYDGEAFNSSPAGKL
                     LAAADPDGVGIELDGTQIVAIAAFLRVINALENIRQSVELLESSRNKGIARRTEAKEL
                     IARSLNETQDSIRVLEGGGLHPEAVAHLREAQTFAKKAARSLFFRDRLTKQAIEEQNK
                     ARVQLVESS"
     gene            2287..2688
                     /locus_tag="VE030_07785"
     CDS             2287..2688
                     /locus_tag="VE030_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23350.1"
                     /translation="MRKLLAGIAVMGLWLACLAQAHAQQMTEMYIPIGQSPGVSGKSS
                     LMGAIESVNPQKHTLAVSGPSGIHEILLTDRTRIWLDRTQARLPNLTGTLANLHKGLK
                     VEVKFRDADQKRVAEWIKVQVAESGAPARKP"
     gene            complement(2727..3542)
                     /locus_tag="VE030_07790"
     CDS             complement(2727..3542)
                     /locus_tag="VE030_07790"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HZF23351.1"
                     /translation="MTGLGTEIVGTVVIVAAVILANIVISAALRGRGWLSRETKLRAS
                     VFWRNFSFLIAFLALLFIWRTELRAAALSLAALSVALVLGGKELLTSMLGYIHRTTSG
                     SFEFGDVIEINGIKGEVIDQTLFSTTVLEMSDEHLFTGRVVQFPNSFYVSQALKNHSR
                     LGNYQLALVAVPLAPGSSLETAKEVLIEAARVVCSEFVAPAQKALRDLEGEQFIVMPS
                     ADPRVTVRLGDAGKVTLLLRFPCLTNQRTRTEQDILARYLKAVPGDGKTRDEG"
     gene            complement(3794..6253)
                     /gene="lon"
                     /locus_tag="VE030_07795"
     CDS             complement(3794..6253)
                     /gene="lon"
                     /locus_tag="VE030_07795"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HZF23352.1"
                     /translation="MNFWPTMQKMLSPDDAGKSAPEPNAGRSATALKPLPEDALIIIP
                     LRNAVLFPGIMSPVTVGRPSSVAAAQEAARSERRVGFLLQRDPEKNELKPDDLHWVGT
                     AGQVVRYITGAEGAHHLVVQGQSRFRVLEFLDGWPFMVARVAIVEPAEESSPEIEARF
                     LQLKERAVEAIQLLPNVPDELAGVVQAMNSPALLADMVANLLDVKNEEKQTVLETFDL
                     KRRLDKVLALLGERVEVLRLSKEIGDKTRKEFDERQREHVLREQMRQIQKELGEGEET
                     AAEIEELKKAIDDAGMPEDVHKHAVKELKRLQRMGEGSAEGSMLRTYLEWLSELPWKA
                     EQGRPIDIAEARKVLDEDHYGLEKIKRRILEYLAVKKLNPDGKSPILCFVGPPGVGKT
                     SLGQSIARATGRKFQRVSLGGTHDEAEIRGHRRTYIGALPGNIIQAIRRAESRTGVLM
                     LDEIDKLGAGGFHGDPSSALLEVLDPEQNSKFRDNYLGVDFDLSKVMFIATANVLDTI
                     PGPLRDRMEVIQLPGYTEEEKLEIARRYLLKRQLEANGLKPEQAMLTDAAIKAIIRDY
                     TREAGVRNLEREIGATLRSAAMKIAEVQAQSVVVDAPDLQAILGPRKFEDELALRTAL
                     PGVATGLAWTPVGGDILFIEATKVPGSGKLILTGQLGEVMKESAQAALTLAKMYSGES
                     LEKTDLHVHVPAGATPKDGPSAGVAMFLALVSLLAHKPVRSDVAMTGEISLRGLVLPI
                     GGVKEKVLAALRAGIRTVMLPKRNQKDLEDVPAAAREKLEFVWLETVEDAMRCAVGGD
                     PAQLFKSKDEGDNTPGGLVAA"
     gene            complement(6250..6669)
                     /locus_tag="VE030_07800"
     CDS             complement(6250..6669)
                     /locus_tag="VE030_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HZF23353.1"
                     /translation="MTSRDLTRLMWSEALSLLEQADRLHRQFIRPAAAEAPAWEPPVD
                     IVETADEVRVHVALPGAAADSVAVKLEPGEITISAHRPFPLRTSESECTARIHALEIP
                     YGRFERRIGLPTQALALAGRKLQDGCLTLIFSKKESS"
     gene            complement(6875..8179)
                     /locus_tag="VE030_07805"
     CDS             complement(6875..8179)
                     /locus_tag="VE030_07805"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HZF23354.1"
                     /translation="MIGKSFQRISTRDLWMAVAPALVAVVLALVFAFRFIEPAPPDRV
                     VMATGAVDGGYHMFAQRYKEILARDGVTVDLRISAGSQENVSRLLDEKSDVEVGFFQG
                     GSAFAANAPNLRSLGSIYYEPLWVFYRGADIHDFGGLKGKRLAIGPEESGTHALALQM
                     LAVNATVMPPTVLLPESGLKANEMLLQGELDAVFMVGPPESPLVEQLVSARGIRLLSL
                     ERAEAYTRRFPYLTRLTLPRGVFDFVSNNPAQDVTLVSPTANLLARDTLHPALAFLLM
                     RAAREIHGNPGLLNKIGDFPAPLNSEFPLSSEAERYYKTGPPFLQRYLPYWIAVMVDR
                     LWIILLPALALLVPLTKIIPPVYRWRVRSRIYRWYARLKEVELELEDDPPPEKLRELL
                     KRLDQIERSVNGINTPLAYSDNLYAFRQNVNLVRQRVQARLV"
     gene            complement(8261..>8632)
                     /locus_tag="VE030_07810"
     CDS             complement(8261..>8632)
                     /locus_tag="VE030_07810"
                     /inference="COORDINATES: protein motif:HMM:NF022307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2652 domain-containing protein"
                     /protein_id="HZF23355.1"
                     /translation="KFVAHLGEVAIQRVKRISKLAGLDVILVHRMLKNTVPLPEYMLL
                     SEPLFRLSDEKIRTHTQSLPQEFEGLGPVQTYFVDIKDVAAELPPKPEVTRLAQMRET
                     WGVIIRTLPYLIGLKKPRFAV"
BASE COUNT         1652 a         2738 c         2600 g         1642 t
ORIGIN      
        1 agcagccgca agtaccccgc gcctggttag cgcgaaaacg gtgtgcgccc ttccctgtgc
       61 gcgaagagaa aacgctggtg actttttcgt accgcgcaaa cttatggtgt agtacctcac
      121 aaacttatgg tgtaccggat aacaatcgag agtaaatttc tgagagcaga gttgttcgac
      181 cgcgaaacca tggaagaaac aaccgcattt ctcgacgtcg ttactcggga gagcggcatt
      241 catgggcgct caaatatcct catttacgtt cattcgtcag cgccgatatt tcatgttgaa
      301 tatcacgggt tcatcgattg cttcaaggag attgcaaaga aacccggaca ccagatagct
      361 ttgctcgcgg atagcaagga tctatatatt tcgcacgaat acctggagtt gcgcgcgcga
      421 cagtatgggt tgaatgttcg caattttcga aatgaggcgg cagcactcga atggcttgca
      481 aaccggcgtc gggcgccaga ccggcgtggg caacaggaac gacgctggcg tcaggaacct
      541 ctcaagcatc aggaacggcg tctgcacccg gaacggcgtc agaggatgcg gcgtggcggc
      601 ctggaaagtc cgctttagcc cgatagtaat cggcgcagca aaaatgcgga cgcagcccgg
      661 cttctcgtca cgggcttgat catccttgct cttgaatcat gccgcgcacg atttttgcgt
      721 gagcaacgat gtcgaacgtg aaagagcaga caaataacgc gtcccgataa gcaattcagc
      781 gtcggacgac acggcgacct gatgccctgg ctggatagcg cgagcgcgca acggtgcaaa
      841 aagatcagga ggaacaaatg gaaaggcgcg ttcgcggtcg gcttgcagtg gggcccgaag
      901 gcgttctgcg cgcgccggtt gttgcggctt gcctggttat tctcagcgcg tgcaatgcgt
      961 caccttccca ggatcttgtc gccaaggggc gacagatctt cttcaacgaa acctttcagg
     1021 gcaatggcag aacctgcggg acctgtcatc cggccgagaa taacctgacg ctcgatcccg
     1081 cgttcatcgc cacgcttgct ccgaacgacc cgctgttcgt ggccgaattc aacccggacc
     1141 tcaggaagaa tttcgagaat ccgagactga tgcgccagtt cgggctgatc ctggaaaacc
     1201 tggacggttt cgtggacctg gacaagaagt ttgtcatgcg tggtgtgccg catatgctcg
     1261 ggcttcggac ttcggtcgat agcccccaag ggccgcgcac cggctggtcc ggcgatggcg
     1321 caccggggga cggttcgctc cggtcatttg ccaccggcgc agtcatccag catttcacca
     1381 agaccctgaa ccggatcccg gggcaggatt ttcgtctgcc gacggaagag gaactcgacg
     1441 caatcgaggc attctcgctc tcccttggcc ggcagcagga cctggccttg cccctacccc
     1501 tgaagggcat cgtgccaaag cgcgggcagg agatcttcct cgacaactca ctcggaaagt
     1561 gcaaccgctg ccatgccaat gccggggcga ccgccaactt cggcgccggg agcatcggca
     1621 acgctaactt caacacgggg gtggaaaatc ttccggatca gcccgccgac ctgagcggcg
     1681 aattgaatcc agccgatgac ggattccgca ccccgggcga cggtactttc aatacgccgc
     1741 cgctggtcga ggccgccgaa accggcccgt tctttcacaa caacgccatc gagaccatcg
     1801 agggggcagt cgctttttac gacggcgagg cattcaacag ctcacccgcg ggcaaacttc
     1861 tggccgctgc agatcccgat ggcgtcggca tagaactcga cggcacgcag atcgtcgcca
     1921 ttgccgcttt cctgcgggta atcaatgcgc tcgagaacat caggcagagc gtcgaactcc
     1981 tggagtccag ccggaacaaa ggcattgcgc gtcgcacgga ggcgaaggaa ctcatcgctc
     2041 gctcgctcaa cgagacgcag gacagcatcc gggtgctcga gggcggcggt ctgcatccgg
     2101 aagccgtcgc gcaccttcgt gaagcgcaga cgttcgcgaa aaaagcggcc cgcagtctat
     2161 tcttccggga tcgactgacc aaacaggcca tcgaagaaca gaataaggca cgggttcaac
     2221 tcgtcgaatc gtcctgacga gaccgtgccg atgccaatga tcacgaatcg aaggaggttg
     2281 gcagcaatgc gcaagctctt ggcgggaatt gccgtcatgg gactctggct cgcttgtctg
     2341 gcgcaggcac acgcgcagca aatgaccgag atgtatatcc cgatcgggca atcacccgga
     2401 gtgtccggaa agtccagcct gatgggcgcg atcgaatcgg tcaacccgca gaagcacacg
     2461 ctggcggttt ccggaccttc gggaatccac gagatactcc tgaccgaccg cacccgcatc
     2521 tggctggacc ggactcaggc gcgactgccc aacctgaccg gaaccctggc gaatctgcac
     2581 aaggggctga aggtcgaagt gaaatttcgc gacgcggatc agaagcgggt cgcagaatgg
     2641 atcaaggtgc aggtcgccga atccggcgcg cccgcccgca agccatgaca cgcgagtgat
     2701 tgttcacgcc tgtcttgcct catccctcat ccttcatccc tggttttccc gtcacccggc
     2761 accgctttta agtatcgggc caggatgtcc tgctcggtcc gggtacgctg attggtaagg
     2821 cagggaaagc gcaataacag cgtcaccttg ccggcgtcac ccagccgcac ggttacgcgc
     2881 gggtccgcag acggcatcac gatgaactgc tcaccctcca gatcgcgcag ggctttctgg
     2941 gcgggagcaa cgaattccga gcacaccacc ctggccgctt cgatgagtac ctccttcgcg
     3001 gtttcaagac tgctgcccgg cgcgagcggt acagcgacca gcgcgagctg atagttaccc
     3061 agacgcgaat gatttttcag cgcctggctt acgtaaaagc tgttggggaa ctgcaccact
     3121 cgcccggtga aaagatgctc gtcgctcatc tccagcacgg tggtggaaaa cagcgtctgg
     3181 tcgatgacct cgcccttgat accgttgatc tcgatgacgt cgccgaattc gaaacttccc
     3241 gacgtggtgc gatggatgta accgagcatc gaggtcagca gctcttttcc gcccagcacc
     3301 agagccacgc tcaacgctgc gagggaaagc gcggcggcac gcagctccgt gcgccagatg
     3361 aacaacagcg caaggaaggc gatgaggaat gagaagttgc gccagaaaac cgacgcgcgg
     3421 agcttggtct cccgtgacag ccaccccctg ccccgcaacg cggccgagat gacgatgttg
     3481 gcgaggatta ccgcggcgac gatgacgacc gtgccgacga tttccgtccc aagtccggtc
     3541 ataaaggcat cgaataacat attccatggt cttccaggtc aggcagggcc acatggccat
     3601 gccgcagacc cgtcattcta gcgaatgaaa cccgaaaggc taaaggggag aggggaaagg
     3661 gaccgcaact tactcgagat agtttttact ttcacctgct gtttcgcggc gctcagtact
     3721 gatctattgg ctatccttca tcctttgcct ccggcgtaac cttgatccga gcttcgcacg
     3781 gctaaaggtc agcctacgcc gcaacaagcc ctccgggcgt gttgtctcct tcatccttgc
     3841 tcttaaagag ctgcgccggg tcaccgccga cggcgcagcg catcgcatcc tcgacggttt
     3901 ccagccacac gaattccagc ttctcacgcg cggccgcggg cacgtcttcc aggtctttct
     3961 ggttgcgctt cggaagcatc acggtcctga tgcccgcgcg cagcgccgcg agcaccttct
     4021 ccttcacgcc gccgatcggc agcaccaggc cgcgcagcga gatctcaccg gtcatcgcca
     4081 cgtcggaacg caccggcttg tgggcgagca acgacactag cgccaggaac atcgccactc
     4141 cggcgctcgg gccatccttc ggcgtcgcgc ctgcgggcac gtgcacgtgc aagtcggtct
     4201 tctccagcga ttcgccgctg tacatcttcg ccagcgtcaa cgccgcctgt gccgactcct
     4261 tcatcacctc gcccagttgc ccggtgagga tgagcttgcc ggaccccggc accttggtgg
     4321 cctcgatgaa caggatgtcc ccgcccaccg gcgtccacgc caacccggtc gccacgcccg
     4381 gcagcgccgt acgcagcgcc agttcatcct cgaacttgcg cgggccgagg atggcttgca
     4441 ggtccggagc atccacgacc acgctctgag cctgtacttc ggcgattttc atcgccgccg
     4501 aacgcagcgt cgccccgatc tcgcgttcga gattgcgcac gccagcctcg cgcgtgtagt
     4561 cgcggatgat ggccttgatc gctgcatcgg tgagcatcgc ctgttccggc ttgaggccgt
     4621 tggcttcgag ctggcgcttg agcaggtaac gcctggcgat ctccagcttc tcttcctcgg
     4681 tgtaacctgg aagctggatc acctccatgc gatcgcgcag tggccctgga atcgtgtcga
     4741 gcacgttggc ggtggcgatg aacatcacct tcgacaggtc gaagtccacg ccaaggtagt
     4801 tgtcgcggaa tttcgagttc tgctccgggt cgagcacctc cagcagcgcg ctcgacggat
     4861 ctccatggaa accgcctgcg ccgagcttgt cgatttcgtc cagcatcagc acgccggtac
     4921 gcgattccgc acgccggatc gcctggatga tgttgcccgg cagcgcgccg atgtaggtac
     4981 ggcggtggcc acggatctcc gcctcgtcgt gggtgccgcc cagagacacg cgctggaact
     5041 tgcgcccggt ggcacgtgcg atcgactgcc caagcgaggt cttgcccaca cccggcgggc
     5101 cgacgaagca caggatcgga ctttttccat ccgggttgag cttcttcacc gcaagatatt
     5161 ctaggatgcg gcgcttgatc ttctccaggc cgtagtgatc ctcgtcgagc accttcctcg
     5221 cttccgcgat gtcgatcggc ctgccctgct ccgctttcca gggcaattcg ctcaaccact
     5281 ccagataggt gcgcaacatc gagccctcgg cgctgccttc gcccattctc tgcaggcgtt
     5341 tgagctcttt cacggcgtgc ttgtgcacgt cctcgggcat gccggcatcg tcgatggcct
     5401 tcttcagttc ctcgatctcg gctgcggttt cctcgccctc gcccagttcc ttctggatct
     5461 ggcgcatctg ctcgcgcagc acgtgctcgc gctggcgctc gtcgaactcc ttgcgggtct
     5521 tgtcgccgat ctccttggaa agcctcagca cctcgacacg ctcgcccagc agcgccagca
     5581 ccttgtcgag gcgccgcttg aggtcgaagg tttcgagtac ggtctgtttt tcttcgttct
     5641 tcacgtccag caggttggcc accatgtcgg ccagcagcgc gggcgaattc atcgcctgca
     5701 ccacgcccgc cagttcgtcg ggcacgttcg gcagcaactg aatggcctcg accgcacgct
     5761 ctttcagctg cagaaagcgc gcctcgatct cggggctgga ttcttccgcc ggctccacaa
     5821 tcgccacgcg ggccaccatg aagggccagc cgtccagaaa ctccagtacg cggaaacgcg
     5881 actgcccctg gaccaccagg tggtgggcgc cttcggcgcc ggtgatgtag cgcactacct
     5941 ggccggcggt gccgacccag tgcaggtcgt ccggcttgag ttcgtttttc tccgggtcgc
     6001 gttgcaacag aaatccgact cggcgctcgc tgcgggccgc ttcctgcgcc gcagcgaccg
     6061 aggacgggcg accgaccgtg accggcgaca tgatgcctgg gaaaagcacc gcattgcgca
     6121 gcggaatgat gatcagcgcg tcctccggca gcggtttcaa tgccgtagcg ctgcgcccgg
     6181 catttggctc cggtgcggac ttgcctgcgt cgtctggcga aagcattttc tgcatcgtgg
     6241 gccagaaatt catgaagact ccttcttgct gaaaatgagc gtgagacacc cgtcctgcaa
     6301 cttcctgccg gccagtgcga gcgcctgcgt gggcaggccg atccggcgct cgaagcgtcc
     6361 gtacggaatt tccagcgcat ggatgcgcgc cgtgcattcg ctctcggatg ttcggagcgg
     6421 gaatggcctg tgtgcggata tggttatctc accgggttcg agcttcaccg cgaccgaatc
     6481 agcagccgcg cccggcagcg ccacgtgcac ccggacttcg tcggcagtct cgacgatgtc
     6541 caccggcggc tcccaggcag gcgcttcggc cgcagccggg cgaatgaact gccggtgcag
     6601 ccggtcggct tgctccagga gcgaaagggc ttcgctccac atcaggcggg taaggtcgcg
     6661 cgaggtcatc cagaggtctc cgtaatgaac cctacatggc ggcgatcagg ccggaatcaa
     6721 gcgctctgtc cggtagggga atatctggtc agatcagtga cttcggtccg gtcgccggat
     6781 cgcgaactgg cggggctgct cgaaaaacgg ggtcgaggct cgagtgccgg gtcctgcctt
     6841 caggccgtca cgtacgctac aaccttgccg gactttacac caatctcgcc tgcactctct
     6901 ggcgtaccag attcacgttc tgccggaagg cgtaaaggtt gtccgaatac gcgagcggcg
     6961 tattgatgcc attgaccgag cgttcgatct ggtccagccg cttcagcagc tcccgcagtt
     7021 tttccggcgg gggatcgtct tcgagttcga gctccacttc cttgagccgt gcgtaccagc
     7081 gatagatgcg cgagcgcacg cgccagcggt acaccggcgg aatgatcttc gtgagcggca
     7141 ccagcagcgc cagcgccggc agcaggatga tccacagccg gtcgaccatc accgcgatcc
     7201 agtatggcag gtagcgctgc aggaacggcg gcccggtctt gtagtaacgc tccgcctcgc
     7261 tgctcagcgg gaactcggag ttcagcggcg ccgggaagtc gccgatctta ttcagcaacc
     7321 ccgggtttcc gtggatttcc ctggcggcac gcatcagcag gaacgccagc gccggatgca
     7381 gcgtgtcgcg ggcgagcagg ttggcggtgg gtgacaccag cgtcacgtcc tgcgccgggt
     7441 tgttgctgac gaagtcgaac acccctcgcg gcaaggtcag cctggtcagg taaggaaagc
     7501 ggcgcgtata ggcttccgcg cgctcgaggc tgagcagacg gatacccctg gcggacacca
     7561 gttgctcgac cagcggcgac tccggcggac ctaccatgaa caccgcatcg agttcgcctt
     7621 gcagcagcat ttcgttggcc ttcagtccgc tctcgggaag gagcacggtc ggcggcataa
     7681 cggtggcatt caccgccagc atttgcagcg cgagcgcgtg cgtgccgctt tcctcgggtc
     7741 caatcgccag ccgtttgcct ttcaggccac cgaaatcatg aatatccgcg ccgcggtaga
     7801 acacccatag cggttcgtaa tagatgctgc ccaacgaacg caggttcggc gcattggcgg
     7861 caaaagcgga gccgccctgg aaaaagccca cctccacgtc ggatttttcg tccagcaggc
     7921 ggctcacgtt ctcctgcgag ccggccgaga tgcgcagatc gaccgtgacg ccatcgcgcg
     7981 ccaggatctc cttgtagcgc tgggcgaaca tgtgataacc gccgtccacc gcgccggtcg
     8041 ccatcaccac tctgtccggc ggcgccggct cgatgaaacg gaatgcgaag accagagcca
     8101 gtacgacggc aacgagtgcc ggcgcgaccg ccatccagag atcgcgcgta ctgatccgct
     8161 ggaacgattt tccgatcatg cggcgattgt agccggaggc cgctggagag gcgcgggggc
     8221 cgattgtggc ggcgctctgt gcgccgtcca actcggtgat tcaaaccgca aagcgcggtt
     8281 tcttgagccc gatcaggtag ggcagcgtcc gaatgatgac gccccaggtc tcgcgcatct
     8341 gggcaagccg ggtgacctcg ggtttgggcg gaagctcggc cgcgacgtcc ttgatgtcga
     8401 cgaaataggt ctgcaccggt cccaggcctt cgaattcctg cggcagcgac tgggtgtgcg
     8461 tgcgaatctt ttcatcgctc aggcggaaca acggctcgga cagcagcatg tactccggca
     8521 gcggcacggt attcttgagc atgcggtgga ccaggatcac atccagtccg gcgagcttgc
     8581 tgatccgctt tacgcgctgg atggcgactt cgccgaggtg cgccacgaac tt
//
LOCUS       DASQOP010000188         3284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1488621, whole genome shotgun sequence.
ACCESSION   DASQOP010000188 DASQOP010000000
VERSION     DASQOP010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1488621"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VE030_07815"
     CDS             complement(<1..190)
                     /locus_tag="VE030_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23356.1"
                     /translation="MDISILQAATQLLDQRLSLTPITDLPEPLRPRDESQGYQLQRVL
                     NGLLTVAGMGEQVGHKIGC"
     gene            464..1939
                     /locus_tag="VE030_07820"
     CDS             464..1939
                     /locus_tag="VE030_07820"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZF23357.1"
                     /translation="MRDQSTLLRRPSLLKGLLLSIATAVAVASAGVAGAQNGAKTAVE
                     AAKKICSGKTITIVWEAGLQSLDPLNFSGPLWEKETGCKVKVVEVPTAEMFTKIMQEY
                     RAGTGAYDALNVIPAWMPDLAQAGALEVLDPLVDKYGFRSELQLIAPTYRDNQMTVNG
                     KIYGFPDDGDVFLFYYRKDIFARDDLKKDFKAKHGYELAPPKTWKQFDDIGSFLTEKL
                     KKEGIYGAAFFREPPYTMFMFQERFRVEGGKFFDADMKAAVNSPIGVKVFTEMRKENR
                     FMPPGVEKFGFVENLATFLKGESAMTISWPPYGRFAAGYLANEKALDWVPKSTIAGKV
                     GYALPPGGHPELAAGFALSVASTSKNKEAAYLFIQWLNSEEISGKRVRLPYALRDPFR
                     DSHFKDPEYMKLWPDAKDYLAALGQGAKTGLLDLSLIQTDKYEEALRQGISRLWAGED
                     PKKILDDVAKQWDGVTMQVGVNKQKAVYAAWAKKSGAYPKK"
     gene            2074..2970
                     /locus_tag="VE030_07825"
     CDS             2074..2970
                     /locus_tag="VE030_07825"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HZF23358.1"
                     /translation="MTSLSATSRASFGERNFKYLLIGPAIFVLLLIGIFPLVYLLLVS
                     FQGITMTETSTDFIGFANYTQLFEDTRLWEALLHTVIFTVTALPLELLLGLAMAQLFL
                     ERLPGRQIFIALLVLPVVVSPIVSGATWSLMFNHRFGPINQIIGWIWGEDVTLLWTIS
                     PHLVYPAILAAEVWQWTPFMFLLLLAALANVDKSQLEAAAIDGAGWWRTFFRIVLPAI
                     WPVMAIAILIRGLDLFRLFDIVWALTKGGPGTMTETISIFTYIKGFQQFETSYTAAVA
                     FLVIILLSVVVMLALKRVELSR"
     gene            2967..>3284
                     /locus_tag="VE030_07830"
     CDS             2967..>3284
                     /locus_tag="VE030_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23359.1"
                     /translation="MMTVKQRYALRTGVRLAAAVAVTALFLFPIYWLFMISFKTPEEI
                     YSFPPKWYPGRIHFDNYAVLFKDGDAITVWNSFVVAGVSTVIAMFLGTIGAYSLARFR
                     TGGE"
BASE COUNT          651 a          978 c         1000 g          655 t
ORIGIN      
        1 tgcagccgat cttatggccc acttgttcgc ccattcccgc cacggtcagc aggccattga
       61 gcactctctg caattgatag ccctgcgatt cgtcccgggg tcgcaggggt tccggcaggt
      121 cggtgatcgg cgtaagcgac aaccgctggt cgagcaactg cgttgccgcc tggagtatcg
      181 agatgtccaa ggaagttcct tcgaagtatg cggaaagcgg aaacgtgtat tgcaccacaa
      241 aagtccccct gggacgcgag gccgaggttt ccctgtgcgg gcgaatcgtc gatgcatcgg
      301 ggactctttc cagccctggc ttcgctgcaa tgcggcgaaa gtgcgcttgt tccgggtttg
      361 cttgacactc tggggggcgg ttcaacataa tcggcacgct gtgctgaatc gctcaccata
      421 aagagcgaac aatcaaaaac ttataactca cggaggaggg atcatgaggg atcagtcaac
      481 actccttcgc cggccaagtc tgctcaaagg tttgctgctg tcgatcgcta ctgccgtggc
      541 tgttgcctcg gctggcgtag ccggtgcgca aaacggagcg aagactgccg tcgaagccgc
      601 caagaaaatt tgctccggca agaccatcac catcgtctgg gaagcgggtt tgcagtcgct
      661 cgacccactg aatttctcgg gaccgctgtg ggagaaggaa accggctgca aggtgaaagt
      721 ggtcgaagtg ccgaccgcgg aaatgtttac caagatcatg caggaatatc gcgccggcac
      781 cggcgcgtac gacgcgctca acgtcattcc cgcgtggatg cccgacctgg cgcaggccgg
      841 cgcgctggaa gttctggatc cgctggtgga caagtacgga ttccgttccg agctgcaatt
      901 gatcgcgccc acctaccgtg acaaccagat gacggtaaat gggaaaatct acggcttccc
      961 cgatgacggc gacgtgttcc tgttctacta ccgcaaggac atctttgccc gcgacgatct
     1021 caagaaggac ttcaaagcca agcatgggta cgagctcgca ccgccgaaga cctggaagca
     1081 gttcgacgat atcggatcgt tcctcaccga aaaactgaag aaggaaggca tctacggcgc
     1141 cgcgttcttc cgcgaaccgc cgtacaccat gttcatgttc caggagcgct tccgtgtcga
     1201 aggcggcaag ttcttcgacg ccgacatgaa ggcagccgtg aacagcccga tcggagtcaa
     1261 ggtgttcacc gagatgcgca aggagaaccg cttcatgcct cccggtgtcg agaagttcgg
     1321 cttcgtcgag aacctggcga ccttcctcaa gggcgaaagc gcgatgacta tctcgtggcc
     1381 gccttatggc cgcttcgcgg ccggttatct cgccaacgag aaggcgctcg actgggtacc
     1441 gaaatccacg atcgccggca aggtgggata tgcgctaccg cccggcgggc atcccgaact
     1501 ggccgcgggc ttcgcgctgt cggttgcctc caccagcaag aacaaggagg ccgcctatct
     1561 gttcatccag tggttgaaca gcgaagagat cagcggcaag cgcgtaagac tgccgtatgc
     1621 actgcgcgac ccgttccgcg actcgcactt caaggatccg gagtacatga agctgtggcc
     1681 ggacgcgaag gactatctcg cggcattggg ccagggcgcg aaaaccggtc tcctggatct
     1741 ttcgctgatt cagaccgaca agtacgaaga ggcgctgcgc cagggcatca gccggctgtg
     1801 ggcgggcgaa gatccgaaga aaattctcga cgacgtggcc aagcagtggg atggagtcac
     1861 catgcaggtc ggggtgaaca agcagaaggc cgtgtacgcc gcctgggcca agaaatccgg
     1921 cgcctacccg aagaaatgaa ctggcggccc gcgtcgcctt cataagatgg atcgtccgcg
     1981 gcgggcgcca atcggagcgg ccgcggcgag acaaaatggt gggagcgccg tcacgctggc
     2041 gctccctttt ttgtctctgc aagggctctg acattgactt cactatcggc aacctcccgc
     2101 gccagcttcg gcgagcgcaa tttcaaatac ctgctgatcg ggccggccat attcgtgctg
     2161 ctgctgatcg gcatattccc gctggtctat ctgctgctgg tgagcttcca gggcatcacc
     2221 atgaccgaaa ccagcaccga tttcatcggc ttcgccaatt acacgcagtt gttcgaggac
     2281 acgcgcctgt gggaagcgct gttgcacacc gtgatcttca ccgtgaccgc actgccgctc
     2341 gaactgctgc tcgggctggc gatggcgcag ttattcctcg agcggctgcc gggccggcag
     2401 attttcatcg cgctgctggt cctgccggtg gtggtatcgc cgatcgtctc gggcgcgacc
     2461 tggtcgctca tgttcaacca ccgcttcggg ccgatcaacc agatcatcgg ctggatctgg
     2521 ggcgaggacg tgacgctgct gtggaccatc agcccgcacc tggtctatcc ggcgatcctg
     2581 gcggccgagg tctggcagtg gacgccgttc atgtttctgc tgctgctcgc cgcactcgcc
     2641 aatgtggaca agtcgcaact cgaggccgcc gcgatcgacg gtgccggctg gtggcgcaca
     2701 ttcttcagga tcgtgctgcc ggcgatctgg ccggtgatgg cgatcgccat cctgatccgc
     2761 ggactggacc tgttccgcct gttcgacatc gtctgggcac tcaccaaggg cggccccgga
     2821 accatgaccg agaccatttc gatcttcacc tacatcaagg gtttccagca attcgagacc
     2881 agttacaccg cggcagtggc cttcctggta atcatcctgc tgtcggtggt agtgatgctg
     2941 gcgttaaaga gggtcgaatt gtccagatga tgaccgtcaa gcaacgctac gcactgcgca
     3001 ccggtgtgcg tctggccgcc gcggtggcgg tcacggcgct gttcctgttc ccgatctact
     3061 ggctgttcat gatttctttc aagacgccgg aggagatcta ctcgttcccg cccaagtggt
     3121 atccgggccg gatccatttc gacaattacg cggtgctgtt caaggacggc gacgcgatca
     3181 cggtatggaa cagcttcgtg gtcgccgggg taagcaccgt gatcgcgatg ttcctgggta
     3241 ccatcggcgc ctacagcctg gcaagattcc ggaccggcgg tgaa
//
LOCUS       DASQOP010000189         2468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_81330, whole genome shotgun sequence.
ACCESSION   DASQOP010000189 DASQOP010000000
VERSION     DASQOP010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81330"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VE030_07835"
     CDS             <1..345
                     /locus_tag="VE030_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006416029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyquinol 1,2-dioxygenase"
                     /protein_id="HZF23360.1"
                     /translation="SDVTWNPSVTSVCRNSLFCPTTEEYILPIIHGNDRVEWFVQLSD
                     EITWEVADRETGRPRARLTMKAGDVAAMPADIRHQGFSKKRSMLLVWENATPDLPKRY
                     ERGELKPYPVEF"
     gene            493..1959
                     /locus_tag="VE030_07840"
     CDS             493..1959
                     /locus_tag="VE030_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014900394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZF23361.1"
                     /translation="MQNNLFIDGEFVDAESKARIPVLNPHDNSLITEVAEAQKADIDR
                     AVEAARKAFPAWSKMAAADRGRLLLKLADAIEANADELAKLESLDTGHPLRDTRGLDV
                     VRTALTFRYFGGMADKFQGSVVPVEQGFLNYVLREPVGVVGQIVPWNFPLMFTSWKMG
                     PALAAGNTVVLKPAELTPLSSLRVAALAREVGFPKGVMNIVPGYGNIAGQYLAEHAGV
                     DKIAFTGSTAVGRKIVQASSGNLKRVQLELGGKGANIVFDDAFMPAAINGSAFAIFHN
                     QGQACIAGSRLILHEKIAEQFLDGFLKLARSIKLGNPMNADTEMGPLTSQMHRDRVLA
                     YVKVAKEQGGEILLGGKPPERDDLSKGCYVEPTVVRAKPTDRVSQEEVFGPFVTVTTF
                     RDDEEVLAIANGTDYGLGGGLWTTNLQRAHRTAAAMRSGMVWINCYKRVNPGSPFGGI
                     GRSGYGREMGFEAMHDYTEAKSVWVNVDAKIPPYYPRG"
     gene            2142..>2468
                     /locus_tag="VE030_07845"
     CDS             2142..>2468
                     /locus_tag="VE030_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HZF23362.1"
                     /translation="MINKIVSTVEEAVAGIDDGATVMIGGFGPAGQPAELVDALIAQG
                     AKDLTIVNNNAGNGETGLAALLKARRVRKIICSFPRQVDSQVFDALYRAGDIELELVP
                     QGNLAER"
BASE COUNT          485 a          768 c          794 g          421 t
ORIGIN      
        1 tcggacgtga cctggaaccc ctcggtgacc tcggtgtgca ggaacagcct gttctgcccg
       61 accaccgagg agtacatcct gccgatcatc cacggcaacg atcgcgtcga gtggttcgtg
      121 caactctcgg acgagatcac ctgggaagtc gcagatcgcg agaccggacg gccgcgcgcg
      181 cgcctgacca tgaaggccgg cgacgttgcg gcgatgccgg ccgacatccg ccaccaggga
      241 ttctccaaga agcgctcgat gctgctggtg tgggagaacg ccacgcccga cctgccgaag
      301 cgctacgagc gcggggaact caagccgtat ccggtggagt tctgaaaaag gcaggggaga
      361 ggggagaggg acgaggggcg agggaacgaa cgccgcgacg cggcgccgtt cttacccttc
      421 actcattcac tcattcaccc ttcactgtct ctcacccctt cacccttcac gccttcacga
      481 agaatgcaca tgattcaaaa caaccttttc atcgacggcg agttcgtcga tgccgagtcc
      541 aaggcgcgca taccggttct caatccgcac gacaactcgc tcatcaccga ggttgccgag
      601 gcgcagaaag ccgatatcga tcgcgccgtg gaggccgcgc gcaaggcgtt ccccgcctgg
      661 tcgaaaatgg ctgccgcgga tcgcggccgc ctgctgctga aactggccga cgcgatcgag
      721 gcgaacgcgg acgagctggc caagctggaa tcgctcgata ccggccatcc gctgcgcgac
      781 acccgcggcc tggacgtggt gcgcaccgcg ctgaccttcc gctacttcgg cggcatggcc
      841 gacaagttcc aaggcagcgt ggtgccggtg gagcagggct ttctaaacta cgttttgcgt
      901 gaaccggtcg gcgtcgtcgg ccagatcgtg ccgtggaact tcccgctgat gttcaccagc
      961 tggaagatgg gtccggcgct cgcggcgggc aacacggtgg tactgaaacc ggcggaactc
     1021 accccgctgt ccagcctgcg cgttgccgcg ctggcgcgcg aggtcggctt cccgaaagga
     1081 gtgatgaaca tcgtgccggg ctacggcaac atcgccggcc agtaccttgc cgagcatgcg
     1141 ggcgtggaca agatcgcctt caccggctcg accgcggtgg ggcgcaagat cgtgcaggcg
     1201 tccagcggca acctcaagcg ggtgcaactc gagctgggcg gcaagggcgc caacatcgtc
     1261 ttcgacgacg cgttcatgcc ggcggcaatc aacggttcgg cgttcgccat cttccacaac
     1321 cagggacagg cctgcattgc cgggtcgagg ctgatcctcc acgaaaaaat cgccgaacag
     1381 tttctcgacg gctttctcaa gctggcgcgc tcgatcaagc tcggcaatcc gatgaatgcc
     1441 gataccgaga tggggccgct cacctcgcag atgcaccgcg accgcgtgct cgcctacgtc
     1501 aaagtggcaa aggaacaggg tggcgaaatc ctgctgggcg gcaagccccc ggaacgcgac
     1561 gatctttcca aaggctgcta cgtcgaaccg accgtggtcc gtgcgaaacc aaccgaccgg
     1621 gtctctcagg aggaagtgtt cggacccttc gtcaccgtca ccactttccg cgacgacgaa
     1681 gaagtgctgg cgatcgccaa cggcaccgat tacggcctgg gcggcgggct gtggaccacc
     1741 aacctgcagc gcgcgcatcg caccgcggcg gcaatgcgca gcggcatggt ctggatcaat
     1801 tgctacaagc gggtgaatcc cggctcgccg ttcggcggca tcggcaggtc ggggtacggg
     1861 cgcgagatgg gtttcgaggc gatgcacgac tacaccgagg cgaaatcggt gtgggtcaac
     1921 gtggacgcca agattccgcc ttattacccg cgcggttgat gcacaagaat tttcaccaca
     1981 gagacacaga gtcacagaga aaggcgtaga gaaaattggg aaatgaaccg cgggtcaagg
     2041 cgtggcagtt tcccgaacgt gtcttggctc gggctttgtt ccggggcctc gttcagtttt
     2101 cctccgtgcc tctgtgtctc tgtggtcaaa gggtctgaac catgatcaac aagattgttt
     2161 cgacggtcga ggaagcagtg gcgggcatcg acgacggtgc cacggtgatg atcggcggct
     2221 tcggtcctgc gggtcagccg gcggaactgg tcgatgcgct gatcgcgcag ggcgcgaaag
     2281 acctgaccat cgtcaacaac aatgccggca acggcgagac cggcctcgcg gcgctgctca
     2341 aggcgcggcg ggtgcgcaag atcatctgct cttttccgcg ccaggtggat tcgcaggtgt
     2401 tcgatgcgct ctatcgcgcc ggcgacatcg agctggaact ggtgccgcaa ggcaatctcg
     2461 ccgagcgc
//
LOCUS       DASQOP010000190         4884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2094188, whole genome shotgun sequence.
ACCESSION   DASQOP010000190 DASQOP010000000
VERSION     DASQOP010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2094188"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VE030_07850"
     CDS             <1..215
                     /locus_tag="VE030_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HZF23363.1"
                     /translation="RRWYWEQLAWISLATLAYLPATAAPIGRTSGGLPVGMQIIGPYF
                     EDRSCIEFAKLLADVAGGFEAPPGFS"
     gene            complement(235..693)
                     /locus_tag="VE030_07855"
     CDS             complement(235..693)
                     /locus_tag="VE030_07855"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HZF23364.1"
                     /translation="MLSVVFALAACAGSPPRVASATAAASSDNVDVSRAADHALAMVG
                     APYRYGGVDPGGFDCSGLVHYSFRKIGIALPRDTYSQRKVGIEIEMDDLAKGDLVFFD
                     QEGKKSSHVGIYLGDGRFVHAPSTGGKVRADKIDLTYWRKHLNEARRIRM"
     gene            complement(1003..2460)
                     /locus_tag="VE030_07860"
     CDS             complement(1003..2460)
                     /locus_tag="VE030_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013951859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HZF23365.1"
                     /translation="MLTSKTVRRLAIAGILPVLIAAYYAVAVPGTPPAHAGAQSGELQ
                     NAATATQQLPDFTRIVEQNGPAVVNVSVSRKAQAGIQTPFQGIPEDDPMFEFFRRFAP
                     PQQQGEQPMQRGQGSGFIVSADGVILTNAHVAADATDIVVKLTDRREFEAKVIGVDRR
                     SDIAVLKIEAKNLPVVKIGDPARLKVGEWVAAIGSPFGLENTVTSGIVSAKARALPDE
                     NYVPFIQTDVAVNPGNSGGPLFNMNGEVVGINSQIFSRTGGYMGLSFAIPIDVAMNIK
                     DQLAHNGKVTRGRIGVQIQEVNQALANSFGLPKPQGALVSAVEPGSPADKAGLKSGDV
                     VLGVNGRDIDQLSELTSTIAGIKPGTNARLQVWRNGSRQDINVTVGELSEQKVAALGA
                     GEPGGADSLGLTVRELVPEERAKLKTEGGLVVQNTSGAAARAGIHAGDVILALNDTPV
                     KSVDQLKQLVKKSSKTVALLVQRDEARIYVPVHIG"
     gene            complement(2950..4017)
                     /locus_tag="VE030_07865"
     CDS             complement(2950..4017)
                     /locus_tag="VE030_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23366.1"
                     /translation="MTGASMPQSMAVGPDHVDITWMSIANIHYELGPLRILTDGYISR
                     IPEREFHGGGGGYAYTRHPWKPDVAAVSRVLDAIGGASEVNLLLTGHSHWDHSFDTAT
                     WSKLTNAPIIGSITTCFEVMAENIPAERCTPVYGLEKIPLGEGVTLRVVRWNHSGDPA
                     KNWEQHDPVELDAVPRPDPATGGLRAGVAEDFPNGGGNRGFLFTVEGRQGRFSWFYQN
                     SASAIDLHVPIIINGVDYGAPIENLKIAMKDAGLQSVDLWIGTGGVPVAQLVVPVIRP
                     KAYIPIHWDGLWEPFEAGMPWPFSDAALEEILAKSGVKLLKPGQYMDKWRLDRSGIGP
                     VANTRIKRALGFPEVQPFPRQ"
     gene            complement(4295..4819)
                     /locus_tag="VE030_07870"
     CDS             complement(4295..4819)
                     /locus_tag="VE030_07870"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HZF23367.1"
                     /translation="MQFCGVPDTMTAYYGAFPAWISLRLSPLLLIAALAGCAGTPEVA
                     ETPARRASDESALKAVAFAREMIGKPYRYAGDTPAGFDCSGLVRYSYGRAGISMPRAT
                     PAQRNATTLISVRSLRPGDLLFFDQEGKKSSHVGIYLGDGRFVHAPSTGGRVRTDSLS
                     AEFWKKHFVEARRV"
BASE COUNT          904 a         1521 c         1497 g          962 t
ORIGIN      
        1 agcgccggtg gtactgggag caactcgcct ggatttcgct tgccacgctg gcctatctgc
       61 cggcgaccgc cgcgccgatt ggacgtacgt ccggcggact gccagtggga atgcagatca
      121 tcggcccgta tttcgaggac cgcagctgca tcgaattcgc caaattgctg gctgacgtag
      181 ccgggggctt cgaagcgcca ccgggttttt cgtaggcgtc cgggaggagc agcgtcacat
      241 cctgatcctg cgtgcctcgt tgaggtgctt gcgccagtac gtcagatcta tcttgtccgc
      301 gcgtaccttg ccgcccgtcg agggcgcatg cacgaatctg ccgtcaccga ggtagatgcc
      361 gacgtgggag gatttctttc cttcctggtc gaagaacacc agatcgcctt tggcaagatc
      421 atccatttcg atctcgatac cgactttgcg ctgcgaatac gtgtcgcgcg gaagcgcgat
      481 cccgatcttg cggaagctgt aatgaaccag gccgctgcaa tcgaagccgc cgggatcgac
      541 gccgccgtag cgatacggtg cgccgaccat cgccagcgcg tggtcggcgg cgcggctcac
      601 atccacattg tcggagcttg ccgcagcggt tgcagatgcc actcgcggcg gcgacccggc
      661 gcaggctgca agcgcgaaaa cgacgctcaa cattaagacc ggacggaagg atttgcaggc
      721 cacggcgact cctccttttc ggatagtcga cgcaaagtcc atgccgagaa aaatgcgttc
      781 ccggtccatc gatccggcgc ttgtctgacg gtccacgaca cttgggtgac gttcggcgtc
      841 gctccatcga ctgtcgtttg caggcgaccg ggcgtagggc cgcgcgagac ttgtaatttg
      901 cgaaaccgga gcaaggcgaa aagctgatcc cgccgtccgc gcctcacgaa aaaaaagcgc
      961 gccgcgatac gcggcgcgcc ctgatgcagt acttctctta ggtcagccga tatgcaccgg
     1021 cacgtagatc cgcgcctcat cgcgctggac cagcagtgcc acggtcttgg aggacttctt
     1081 caccagttgt ttcagctgat cgacggattt gaccggtgtg tcgttcaacg ccagaatcac
     1141 gtcgcctgca tgaatgccgg cacgcgctgc agcgccggag gtgttctgca ccaccagccc
     1201 gccttcggtc ttcagcttcg cgcgttcctc cggcacaagc tcgcgcaccg tcagcccgag
     1261 gctgtcggca ccgccaggct cgccggcgcc gagcgcggct actttttgct cggagagttc
     1321 gccgaccgtc acattgatgt cctgacggct gccgttacgc catacctgaa ggcgtgcatt
     1381 ggtacccggc ttgatgccgg cgattgtgct cgtcagctct gacaactggt cgatatccct
     1441 gccattgacg ccgagcacca cgtcgcccga cttcaatccg gccttgtcgg cgggactgcc
     1501 tggctccacc gcactgacca gtgcgccctg tggtttgggc aggccgaacg aattggccag
     1561 cgcctggttc acttcctgga tctgcacgcc aatgcgaccg cgcgtgacct tgccgttgtg
     1621 tgccagttgg tccttgatat tcatcgccac gtcgatcggg attgcgaaag agagccccat
     1681 gtaaccgccg gtgcggctga agatctgcga gttgatgccg accacttcgc cgttcatgtt
     1741 gaacagtggg ccgccggaat tgccggggtt gaccgcaaca tcggtctgga tgaacggcac
     1801 atagttctcg tccggcaacg cgcgcgcctt ggcactgacg atgccggagg tgaccgtgtt
     1861 ctccaggccg aacggcgaac cgatggccgc aacccactcg cccaccttga ggcgcgcggg
     1921 gtcgccgatt ttcacgaccg gcaggttctt cgcctcgatt ttcagtaccg cgatgtcgga
     1981 gcgccgatcc acgccgatca ccttggcctc gaactcgcgc ctgtcggtga gtttgacgac
     2041 gatgtcggtg gcgtcggcag ccacatgcgc gttggtgaga atgacaccgt cggcgctgac
     2101 gatgaaaccg gagccctggc cgcgctgcat cggttgctcc ccttgctgct ggggcggagc
     2161 gaagcggcgg aaaaactcga acatcgggtc atcttccggg atgccctgga acggcgtctg
     2221 aatgccggcc tgagctttgc gcgatacgct gacgttgacc acagccggcc cattctgttc
     2281 gacgatccgg gtgaagtcgg gtagttgctg tgttgccgtc gcggcgttct gcaactcgcc
     2341 gctttgcgcg ccggcatggg cgggcggcgt gcccggcacg gcaacagcgt aataggctgc
     2401 gatcagcacg ggcagaatcc cggcgatggc aagccgtctg acagtcttgc tggtaagcat
     2461 cgtgacctcc tgaaatgcaa cgcgggcgga acgcccgcgc tgtgcgaact tgaaattcac
     2521 gacacttcag accagcctgg cgcgccggag ttctcgattt tttcgcacca cggggtcacg
     2581 ccgaccccgc cggttcggta gaagaagtgg ggtcaaacgc aacgccagga accctggttg
     2641 accggtagct ggttgacgcg gctacgccta cgcgttcgac tccgaaagtg cgcatccggt
     2701 tcattcgcgg tcgaaaagtt tcacctccga attcactcac cacggaggac gcggaggaaa
     2761 aacaaaacga ggaaaagcaa atcggggata agtgcgaggt aaccgtttgc gaccctgtgg
     2821 tggcaagttt cgcagcaaca gatcatcaga caatgaaagg tttttacccc cgtttatttt
     2881 cttgctcccg cccttcctcc gcgtcctccg tggtgagcgt ggttcttttc gtttctcgcc
     2941 ggccgccggt tactggcgcg ggaagggctg gacttcgggg aatcccagag cccgcttgat
     3001 gcgggtgttg gccaccggcc cgatgccaga acggtccagc cgccacttgt ccatatactg
     3061 gccgggcttg agcagcttca cgccggactt ggcgagaatc tcctcgagcg cagcgtcgga
     3121 aaacggccag ggcatgccgg cctcgaaggg ttcccacagg ccgtcccagt gaatcgggat
     3181 gtaggccttg ggtctgatca ccggcactac cagttgcgcg accggcaccc cgccggtgcc
     3241 gatccacaga tccaccgact gcaatcccgc gtccttcatc gcgatcttga ggttctcgat
     3301 cggcgcgccg tagtccactc cattgatgat gatcggcacg tgcaggtcta tcgcgctggc
     3361 cgagttctga tagaaccagc tgaaacggcc ttgccgacct tcgacggtaa ataggaagcc
     3421 gcgattgccg ccgccattgg ggaagtcctc cgcaacgccc gcgcgcaatc cgccggttgc
     3481 agggtccggg cggggaaccg cgtcgagctc gaccgggtcg tgctgctccc agttcttcgc
     3541 cggatcgccg ctgtggttcc agcgcaccac gcgcagcgtc accccttcac ccaggggaat
     3601 cttctccagc ccgtatacgg gagtgcagcg ctcggccgga atgttctcgg ccatcacttc
     3661 gaaacacgtg gtgatcgaac cgatgatcgg cgcattggtg agtttcgacc aggtcgccgt
     3721 atcgaaagaa tggtcccaat ggctgtggcc ggtgagcagc aaattcactt cgctggcccc
     3781 gccaattgcg tcgagcaccc gggacaccgc tgcaacgtcg ggcttccagg gatggcgggt
     3841 gtaggcatat ccgccgccgc cgccgtggaa ctcccgttcc gggatgcggc tgatgtaacc
     3901 atcggtaaga atcctcagcg gcccaagctc gtagtggatg ttggcgatgg acatccaggt
     3961 gatatccacg tggtcaggcc ccacagccat gctctgcggc atggatgcac cagtcatcag
     4021 tgcgaaaact attgcggtgg atgttcttct catcaacggc tcccgaagat ccaaagtctt
     4081 accactaagt tccaaagtct caccacggag gacacggagg aaaggcaaga gtaagagaaa
     4141 gcaaacgagt gtaaaacccc caattggtag atgttgcaac aaagtcggaa ttggcggcag
     4201 aagatcacga acccggcttc cattcccaat tcgtttttcc ccgttttttg gttttcctcg
     4261 gtgtcctccg cgtcctctgt agtgagattt acttctatac ccggcgggcc tcgacgaagt
     4321 gtttcttcca gaattccgcg ctgaggctgt cggtgcgcac gcgcccgccg gtggagggcg
     4381 catgcacgaa tctgccgtcc ccgaggtaga tgccgacgtg ggaggatttc tttccttcct
     4441 gatcgaaaaa cagcaggtcg cccgggcgca ggctgcgcac tgaaatcagc gtggtggcgt
     4501 tacgttgcgc cggggtggcg cgcggcatgc tgatgccggc gcggccgtag ctgtagcgca
     4561 ccagcccgct gcagtcgaac cccgcgggcg tatcgccggc atagcgatag ggcttgccga
     4621 tcatctcgcg tgcaaaggca acagccttga gcgccgactc gtcactggcc cgtcttgccg
     4681 gcgtctcggc gacttccggc gtgccggcgc agccggccag cgccgcaatc agcagcagcg
     4741 gcgaaagccg cagcgaaatc caggcaggaa aggcgccgta ataagcggtc atcgtgtccg
     4801 gcactccaca aaactgcatg ctgtgtagga tagcagaaca ctgcgatggt cccggatacg
     4861 gggcagggct tgtttaacgg cccg
//
LOCUS       DASQOP010000191         2597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1692266, whole genome shotgun sequence.
ACCESSION   DASQOP010000191 DASQOP010000000
VERSION     DASQOP010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1692266"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="VE030_07875"
     CDS             <1..919
                     /locus_tag="VE030_07875"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HZF23368.1"
                     /translation="MQGYGRAFLGAGDEIIVSQLEHHSNIVPWQMLCEEKGARLRVIP
                     CTDAGELLLDEYARLFNANTRFVAITHVSNALGTINPVKDMVATAHRHGVLVLVDGAQ
                     AVPHMKVDVQDLDCDFYAFSGHKMCGPTGIGILYGKAQLLEKMKPFEGGGDMILSVTF
                     EKTTYNAIPHKFEAGTPPIAAAIGLGAAVDYLESVGLDRIAAHEHDLLTYATEAFTQL
                     KGVRIVGTAGNKAAVVSFTLQGVHPHDVGTILNEDGVAVRTGHHCAQPVMQRFGIPAT
                     ARASFYLYNTFAEVDVLIAGIRRVQKIFG"
     gene            930..1406
                     /locus_tag="VE030_07880"
     CDS             930..1406
                     /locus_tag="VE030_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HZF23369.1"
                     /translation="MDSKQLYQEVILDHNKKPRNWGKLADASHKAEGLNPLCGDHIWL
                     SLKLDDKRVENIAFEGEGCAICKASSSMMTTNVKGKTVAEAEQMVREFRDMATGKLDI
                     GNLPNHLGRLTVFAGVRDLPSRVKCAILPWHTLQAALHSESVVSTEGDQDPVAAAS"
     gene            1411..1986
                     /locus_tag="VE030_07885"
     CDS             1411..1986
                     /locus_tag="VE030_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HZF23370.1"
                     /translation="MPKIADLEYTPNPNARKFVLKEPLTNGLARSFENAEQAQRDELA
                     SKLFAIPHVSNVFYVDNWITVTQDGEADWQDLLRQIAAPIREAPAAEKASEEYAQAAA
                     WLAKGEGATELSETDKLKLDQINELLDEEVRPYLQGDGGDLYVVGLEGSTLKVHYQGA
                     CGSCPSSISGTLAGIESLVKRVDPEMEVVAV"
     gene            2113..2436
                     /locus_tag="VE030_07890"
     CDS             2113..2436
                     /locus_tag="VE030_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HZF23371.1"
                     /translation="MSDWVFVADAASISPGHYETVDLEGTLAAVFNVDGEFYAIEDVC
                     THDGTVLTSGNPIEGDEIVCPRHGARFCVKTGEALSPPAYEPTATFPVKVEDGKVYVR
                     DNRWD"
BASE COUNT          557 a          762 c          798 g          480 t
ORIGIN      
        1 gatgcagggt tacgggcggg cgttcctggg cgccggcgac gagatcatcg tgtcccagct
       61 agagcaccat tccaatatcg tgccctggca gatgttgtgc gaggagaagg gtgccaggct
      121 gcgcgtgatc ccgtgcaccg acgccggcga actgctgctc gacgaatatg caaggctgtt
      181 caacgccaat accaggttcg tcgccatcac ccatgtctcg aacgcactgg ggactatcaa
      241 cccggtcaag gacatggtcg ccaccgcgca ccgccacggc gtgcttgtgc tggtggatgg
      301 ggcacaggcc gtgccgcaca tgaaagtgga tgtgcaggac ctggactgcg atttctacgc
      361 cttttcaggt cacaagatgt gcggaccgac cggcatcggc attctctacg gcaaggccca
      421 gctcctggag aaaatgaagc ccttcgaggg cggtggcgac atgatcctgt cggtgacgtt
      481 cgagaaaacg acgtacaacg cgatccccca caagtttgag gccggaacgc cgccgatcgc
      541 tgcagcaatc ggactgggtg ctgcggtgga ttacctggaa agcgtagggt tggacagaat
      601 tgccgcgcat gaacacgatc tgctcacgta tgcgaccgag gcgtttacgc aactcaaggg
      661 cgtgcgcatc gtggggaccg cgggaaacaa ggctgctgtc gtgtcgttca cgctgcaggg
      721 cgtacatccc cacgatgtcg gcaccattct gaatgaagac ggcgtggccg tgcgtaccgg
      781 ccatcactgc gcccaaccgg tgatgcagcg cttcggcatc ccggcgacgg cgcgcgcctc
      841 gttttatctt tacaacacct ttgccgaggt ggatgtgctg atcgccggca ttcgcagagt
      901 gcagaaaatt ttcggatagg ccggcttgaa tggattccaa gcagctgtat caggaagtga
      961 tcctggatca caacaagaaa ccgcgcaact ggggcaagct cgccgatgcg agccacaagg
     1021 cggagggcct gaatccactg tgcggcgacc acatctggct ttcgctgaag cttgacgaca
     1081 agcgcgtcga gaacatcgct ttcgagggcg aaggctgtgc gatctgcaag gcctcatcgt
     1141 cgatgatgac caccaatgta aagggcaaga ccgtggcaga ggccgagcaa atggtccggg
     1201 agttccgcga catggccacc ggcaaactcg acatcggcaa tctgcccaac cacctcggca
     1261 ggctcacggt ttttgccggc gtgcgcgatc tgccgtcgcg ggttaaatgc gcgatcctgc
     1321 cttggcacac gctgcaagcg gcgctgcatt ccgaatctgt agtttccacc gaaggcgacc
     1381 aggacccggt tgccgccgca agctgagcca atgccaaaaa tcgcagacct cgaatacacg
     1441 ccgaatccta acgcacgcaa gttcgttctg aaggagccgt taaccaatgg tttggcgcgt
     1501 tctttcgaga acgcggagca agcgcagcgc gacgagctcg cgtccaagct gttcgcgatt
     1561 cctcacgtca gcaacgtttt ttacgtcgat aactggatca ccgtgaccca ggacggcgaa
     1621 gccgactggc aggacttgtt gcgccagatt gctgcgccga tccgcgaggc gccggctgcc
     1681 gagaaagcat ccgaggagta tgcccaggcc gctgcctggc tggcgaaggg cgagggcgcc
     1741 acggaactgt cggagaccga caagctcaag ctcgatcaga tcaatgagtt gctggacgag
     1801 gaagtgcggc cttatcttca gggcgacggc ggagacctgt atgtcgttgg cctggaaggc
     1861 agtaccctga aagtgcacta ccagggcgcg tgtggcagtt gccccagttc catttccggc
     1921 acgctcgccg gcatagaaag cctggtcaag cgcgtcgacc ccgaaatgga agtggtcgcg
     1981 gtctgaaagg ccgtgactga cgtgaatatc gtgaatgtcg taaaaccagg gtgctcgatc
     2041 cagcgaatgg gcactagaag aaagttacga cattcacggc attcacgata gtcacgatct
     2101 tcacggttcc tcatgtccga ctgggtattt gttgccgacg cagccagcat cagtcccggc
     2161 cattatgaaa cggtcgatct cgaaggcaca ttagctgcgg tgttcaacgt cgacggcgag
     2221 ttttatgcca tcgaggacgt gtgcacccac gacggcacgg tgcttaccag cggcaatccg
     2281 atcgagggcg acgagatcgt ctgcccccga cacggcgcgc gcttctgcgt caagaccggc
     2341 gaggctctct cgccaccggc gtacgaaccc actgccacgt ttccagtgaa ggtcgaagac
     2401 gggaaagtct acgtccgcga caatcgctgg gactgaagaa gtccagccgt gtcaccgcgg
     2461 ctgcggacga cccgaaagtc agccgagctc tttgagaaca gcgcgtgcct gcgacacgcg
     2521 ggcggcaatg tcgggcattg cagcttccac ccgaagccga tcctgcccgc tcaacttgaa
     2581 gttcttgcgg ctctgta
//
LOCUS       DASQOP010000192         8866 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2899147, whole genome shotgun sequence.
ACCESSION   DASQOP010000192 DASQOP010000000
VERSION     DASQOP010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8866
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2899147"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VE030_07895"
     CDS             complement(<1..355)
                     /locus_tag="VE030_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23372.1"
                     /translation="MTRLAALLSLGAFVAGNAVPVEQGLGPHEVGVSAPAQITYSLDA
                     RPKPERLDFDFRLGDSHAWINDNGDFQVSSWIKHTGLLCATYRMGLRFGTGSPGCLNV
                     EWITEPLFVTVEIQCN"
     gene            complement(451..1296)
                     /locus_tag="VE030_07900"
     CDS             complement(451..1296)
                     /locus_tag="VE030_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004548450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZF23373.1"
                     /translation="MKLVRYGEAGKEKAGILDAQGAIRDLSGVVKDIDATTVSPEGLA
                     KLRGVKVDGLPKVSGKPRLGPPIANVPKLVCIGLNYADHAKESNLPIPAEPVVFMKAI
                     SAITGPNDDVKLPKGSKKGDWEVELAFVIGKKAQSVSEADALNYVAGYMICNDVSERE
                     WQLEHGSQWSKGKSFDTFAPIGPWFVTADEIEDPQNLSMWLDVNGQRKQTGNTSTMIF
                     GVKKLVSYLSYICTLTPGDVVSTGTPPGVGMGIKPAPQFLKSGDEMRLGIDGLGEQKQ
                     KVIAG"
     gene            complement(1429..2169)
                     /locus_tag="VE030_07905"
     CDS             complement(1429..2169)
                     /locus_tag="VE030_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZF23374.1"
                     /translation="MAGRLSGKVAFVTAAAQGIGRATALAFAREGAKVWATDVNEAKL
                     AELKGKPGIEVRKLDVLNDAEIEKLAKEIGAPNVIFNCAGYVHHGTILQCSDKDWDFS
                     FNLNVRSHYVVIRTFLPKMLEKGGGSIINVASVAGSIKGIPNRFVYGASKAAVIGLTK
                     SVAIDYVKQGIRCNALCPGTVDTPSLGERINAFDDPVQARKDFIARQPMGRLAKAEEL
                     AELSVFLASDESVFMTGQAVIMDGGITI"
     gene            2394..3458
                     /gene="ugpC"
                     /locus_tag="VE030_07910"
     CDS             2394..3458
                     /gene="ugpC"
                     /locus_tag="VE030_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HZF23375.1"
                     /translation="MASVELKQVRKSFGSTQVVHGVDISIADGEFVVLVGPSGCGKST
                     LLRMIAGLEEISEGEIMIGDRVVNNVPPKQRDIAMVFQNYALYPHMKVFDNMAFSMKL
                     AGRSTEEMRQRVDKAAQILGLADYLDRYPRQLSGGQRQRVAMGRAIVRDPQVFLFDEP
                     LSNLDAKLRVQMRTEIKELHQRLKTTSIYVTHDQIEAMTMADTIVVMNGGRVEQIGTP
                     LDLYDEPANLFVAGFIGSPAMNFLPGRIVASPPGRGVQIAEGCVIPVPCSGGLRDREV
                     ILGVRPEHFNIGESCVNVQVIVVEPTGADTQVFCKLAGHNVSAISRERHTFRPGDTIS
                     LKPFEGKTYLFDPDSGARIA"
     gene            3479..4792
                     /locus_tag="VE030_07915"
     CDS             3479..4792
                     /locus_tag="VE030_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZF23376.1"
                     /translation="MTRITRRRFLQASALAGATLGAPMLARDSHAQELSFKPEPGAKL
                     RVLRWKRFVKGDEDQWMANTRAFTARTGVEVTVDNENWEDLRPKAAVAANVGSGPDII
                     LSTNDDSHQYPDKLLEIGDLCEYLGKKYGPWYPSALAYGRNGGRWIALPLGAGGNCMN
                     YRVSHLKAAGFKEFPKDTDGFLKLCKALRKNGTPPGFALGNATGDANGWCHWLIWAFG
                     GKMVDEQNRLALNSKETAAALEYARELYPTFISGTLSWLDPNNNKAFLAGDISLTNNG
                     ISIYYAAKTSQDAKMRELAKDIDHAAFPIGPVGHPTASALFFTSVIFKYTKYPNAAKE
                     YLRFMMEMEQYESWQKAAIGFVTQPLTFYERNPVWTEDPKHTPFKYSCKSMLPNGYAG
                     ALGTASAGCIADFIVVNMVAEAASGAATVKDAMSRAEKRAKRYYK"
     gene            4841..5752
                     /locus_tag="VE030_07920"
     CDS             4841..5752
                     /locus_tag="VE030_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HZF23377.1"
                     /translation="MNNALSRFFDNRNVLGLLFMLPAAALLLVFLTYPLGLGVWLGFT
                     DTKVGRAGGWIGIENYEFLVTDSVFTLSVFNTFVYTVVASVIKFILGLWLALLLNKHI
                     PFKSFVRAIVLLPFIVPTVLSAIAFWWIYDAQFSIVSWVLMKVGIIDQYINFLGEPNL
                     ARMSTIIANIWRGVPFVAICLLAGLQTIPPSMYEAASIDGASPWQQFWHVTLPMLSPI
                     IAVVMTFSVLFTFTDFQLIWVLTRGGPINATHLMATLSFQRAIPGGALGEGAAIATAM
                     IPFLLAAILFSYFGLQRRRWQQGGSDK"
     gene            5867..6736
                     /locus_tag="VE030_07925"
     CDS             5867..6736
                     /locus_tag="VE030_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HZF23378.1"
                     /translation="MASTEPREGMQYLESLPRRWVTVYIPLLVFLFVLLFPFYWMVIT
                     SFKPNAELLSSAGNPFWVINPTLEHFRKLFFDTSYPEWMWNTVLVSVVSTFVSLVASV
                     FAAYAIERLRFRGSRQTGLAIFLAYLIPPSILFIPLATMVFQLGIFDTRWALILTYPT
                     FLIPFCTWLLMGYFRSIPFELEECALIDGATRWQILTKIVLPLAVPGLISAGIFAFTL
                     SWNEFIYALTFISASENKTVPVGVITELVEGDVYHWGSLMAGALVGSLPVAVMYSFFV
                     EYYVAGMTGAVKE"
     gene            6932..8659
                     /locus_tag="VE030_07930"
     CDS             6932..8659
                     /locus_tag="VE030_07930"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HZF23379.1"
                     /translation="MAARRKLRSYEWFGGPGIDSMVHRSWMKNQGLPRHLFEGRPVIG
                     ICNTWSELTPCNAHFRVIADRVKRGVYEAGGFPLEFPVMSLGEPVMRPTAMLFRNLAS
                     MDVEESIRANPLDGVVLLTGCDKTTPALMMGAASCDVPTVVVSGGPMLNGRWRDETVG
                     SGTHVWKFKEMLKRGEMTDEDLLDAEQAMSRSAGHCMTMGTASTMASMAEALGMALPT
                     NAAIPAVDSRRYVLAHMAGQRIVQMVKEGLRMSKILTKQAFENAIRVNGAIGGSTNAV
                     VHLLALAGRIGVKLTLDDWDRLGRDLPCLVNLMPSGEYLMEDFYYAGGLPVVIKELGR
                     AGLLHRKAPTVNGQSIWENVKDAKNWNAKVIFPVDKPFKKQGGIAVLRGNLVPRGAVI
                     KPSAATPKLMKHRGRAVVFENIEDYKKRIDDPKLKVDETCVLVLKNCGPKGYPGFPEV
                     GNFVLPAKILKTGITDMVRISDARMSGTAYGTVVLHSAPEAAAGGTLALVQDGDMIEI
                     DVPKRKLHVDVSDAELARRRKKWGAPKLMADRGYVKIYVDHVNQADEGVDLDFLVGGS
                     GTPLLRESH"
BASE COUNT         1698 a         2638 c         2724 g         1806 t
ORIGIN      
        1 cgttgcactg tatttcgaca gtcacgaaca gcggctcggt gatccattcc acgttcaggc
       61 accccggcga accggtgccg aatcgcaggc ccatgcggta agtggcacac agcaagccag
      121 tgtgcttgat ccaactgctc acctgaaagt cgccattgtc gttgatccag gcgtgcgaat
      181 cgcccaagcg aaagtcgaaa tccagccgct ccggtttggg cctggcgtcc agcgaatacg
      241 tgatctgcgc gggtgcggaa acgccaacct catgcgggcc gagtccttgc tcgaccggca
      301 cggcattccc ggccacaaaa gcgccgagtg acagtagtgc cgcgaggcga gtcatgagag
      361 ttttcctggg gcggtatcgg gccatcatag cccgaatcgg gcgacaaaaa aggcgcaccg
      421 cagtacgcct tttcttggtg ccgagcacca ctaaccagca attacttttt gtttctgttc
      481 acccaggccg tcgattccca gccgcatctc gtcgcctgac ttgaggaatt ggggcgccgg
      541 cttgatgccc atgccgacgc cgggcggagt gccggtgctg actacgtcac cgggtgtcag
      601 cgtgcatatg taggacaggt aggagaccag cttcttcacg ccgaagatca tggtactggt
      661 gttgcctgtc tgcttgcgct ggccgttcac gtccagccac atcgacaggt tctgcggatc
      721 ctcgatctcg tcggcagtga cgaaccaggg gccaatcggg gcaaaggtgt cgaagctctt
      781 gcccttggac cactggctgc cgtgctcgag ctgccactcg cgttcggaaa catcgttgca
      841 gatcatgtag cctgccacgt agttcagtgc gtcggcttcg ctcacgctct gtgccttctt
      901 gccgatcaca aaggcgagtt cgacttccca gtcgcccttc ttcgaaccct tcggcaattt
      961 cacgtcgtcg ttcggtccgg tgatggcgct gattgctttc atgaatacca ccggctcggc
     1021 cgggatcggc agattggact ccttggcgtg gtccgcatag ttcagcccga tgcagacgag
     1081 cttgggcacg tttgctatcg gcgggccgag gcgcggcttg ccgcttacct tcggcaggcc
     1141 gtccaccttc acgccgcgca gcttggccag gccctccggc gacacggtgg tcgcgtcgat
     1201 gtccttcacc acgccggaca ggtcgcgtat cgccccctgc gcatccagaa ttcccgcctt
     1261 ttcttttcct gcttccccgt aacgtaccag tttcattttt tatctcctgc gatgaaattg
     1321 aacttcaaat gcgtgaaggg tgaaagagtg aagggtgaag ggtgaataga gtgaagggta
     1381 agggctaatc tgcttttccc tcatccctca tccctcggcc ctcgggcctc aaatcgtgat
     1441 gccgccgtcc atgatcacgg cctggccggt catgaaaacc gattcgtcgg aggcgaggaa
     1501 caccgacagt tcggcgagtt cctcggcctt tgccagcctg cccatcggct ggcgcgcgat
     1561 gaagtccttg cgcgcctgca ccggatcatc gaaggcgttg atgcgctccc ccagcgacgg
     1621 cgtatccacc gtgcccggac acagcgcatt gcagcgtatt ccctgcttca cgtaatcgat
     1681 cgccaccgac ttggtgagcc cgatcaccgc tgccttcgat gcgccgtaaa cgaaccggtt
     1741 cgggatgccc ttgatgctgc cggccacgga ggccacgttg atgatcgagc cgccgccttt
     1801 ttcgagcatc ttcggcagga aagtgcggat gaccacatag tggctgcgca cgttcaggtt
     1861 gaaggagaag tcccagtcct tgtccgaaca ttgcagtatg gtgccgtggt gaacatagcc
     1921 ggcgcagttg aagatgacgt tgggcgcgcc gatttccttt gcgagttttt cgatttcggc
     1981 gtcgttgagc acgtccagtt tgcgcacttc gatgccgggt ttacccttga gctccgcgag
     2041 ttttgcctcg ttgacgtcgg tggcccagac ttttgcgcct tcgcgcgcga aggcaagcgc
     2101 ggtcgcacgg ccaatgcctt gtgccgcggc ggtgacgaaa gccaccttgc cgctgagtct
     2161 tccagccact gaatctcccc cttttatttc tttggaacga ccgagagtgg caagtatgcc
     2221 acaggcagcc tgctcacctg acctgccctt gctgctgcgc tgcagtttac tttagactgc
     2281 aatggacaaa tccattacaa ggggagaggg ggatggactg cgacacggcc cttcctgtcg
     2341 gcgcgatccg cgcgccgcga ccatcggatc ggcgccgctc caggttcgcg taaatggctt
     2401 cggtcgagct caagcaggtc cgcaaatcct tcggcagtac gcaggtagta cacggtgtgg
     2461 acatctccat cgccgacggc gagtttgtag tgctggtcgg cccaagcggg tgcgggaagt
     2521 ccacgctgtt gcgcatgatc gccggcctcg aggagatttc cgaaggcgag atcatgatcg
     2581 gcgatcgcgt cgtcaacaat gttccgccca aacagcgcga catcgccatg gtctttcaga
     2641 actacgcgct gtatccgcac atgaaggtct tcgacaacat ggccttctcg atgaagctgg
     2701 ccggcagaag cacggaggag atgcgccagc gcgtggacaa ggcggcgcag atcctcggac
     2761 tcgcggatta cctcgaccgt tacccgcgtc agctctcggg cggccagcgc cagcgcgtag
     2821 ccatgggccg cgccatcgtg cgcgacccgc aggtattctt gttcgacgaa ccgctgtcga
     2881 acctggatgc caagttgcgg gtgcagatgc gcaccgagat caaggaactg caccagcggc
     2941 ttaagaccac ttcgatttac gtcacgcacg atcagatcga agccatgacc atggccgaca
     3001 ccatcgtggt gatgaacggc ggccgggtcg aacagatcgg cacgcccctg gatctgtacg
     3061 acgaaccggc caatctgttc gtggccgggt tcatcggttc gccggcgatg aattttctgc
     3121 ccggcaggat agtggccagc ccaccaggcc gcggtgtgca gattgcggaa ggttgcgtca
     3181 tcccggtacc gtgcagcgga gggttacgcg atcgggaggt cattctcggc gtgcgcccgg
     3241 agcatttcaa catcggtgaa tcctgcgtga atgtgcaggt gatcgtggtc gagccgaccg
     3301 gcgcggatac ccaggttttc tgcaagctgg cgggccacaa tgtgtccgcg atttctcgcg
     3361 agcgccacac tttccgtccc ggcgatacca tcagcctcaa acccttcgaa ggcaagacct
     3421 acctgttcga tccggattcc ggcgcacgca tcgcctgatc cggacccgga ggcacaatat
     3481 gactcgaatc acaaggcgtc gttttctcca ggcttccgct ctcgcaggcg ccacactcgg
     3541 cgcgccgatg ctggcgcgcg actctcacgc ccaggagctt tctttcaaac ccgagccggg
     3601 cgcgaaactc cgggtgctgc gctggaagcg cttcgtcaag ggcgacgagg atcagtggat
     3661 ggccaacacc agggcattta ccgcaaggac cggcgtagag gtcacggtgg acaacgaaaa
     3721 ctgggaggac ctgcgtccca aggccgcggt cgccgcgaac gtgggcagcg gacccgacat
     3781 cattctctcc accaacgacg actcgcacca gtatcccgac aaactgctcg agatcggcga
     3841 tctgtgcgaa tacctgggaa agaaatacgg tccctggtat ccgagcgcgc ttgcttatgg
     3901 caggaatggc ggacgctgga tcgcgctgcc gctcggtgcc ggcggtaact gcatgaacta
     3961 ccgcgtcagc cacttgaagg ccgccggctt caaggaattc cccaaagaca cggacgggtt
     4021 cctgaagctg tgcaaggcac tgaggaagaa tggcaccccg cccggattcg cgctcggcaa
     4081 tgccaccggg gacgccaacg gctggtgcca ctggttgatc tgggcattcg gcggcaagat
     4141 ggtggatgaa cagaaccggc tggcgctcaa ttcgaaggag accgcggctg cgctggagta
     4201 cgccagggag ctgtatccga ctttcatttc gggcacgctg tcctggctcg atccgaacaa
     4261 caacaaggcc tttctcgccg gggatatcag cctcaccaac aacggcatct cgatctatta
     4321 cgcggcgaaa acttcgcagg acgcgaaaat gcgggagttg gcgaaggaca tcgaccacgc
     4381 agcctttccg atcggtccgg tcgggcaccc aaccgcatcg gcgcttttct tcaccagtgt
     4441 gatcttcaag tacacaaagt atccgaacgc ggccaaagag tacctgcgct tcatgatgga
     4501 gatggagcag tacgagtcgt ggcagaaggc ggcgatcggt tttgttaccc agccgttgac
     4561 gttctacgag cgcaaccccg tgtggacaga ggacccgaaa cacacgccgt tcaagtactc
     4621 ctgcaagagc atgttgccca acggctatgc cggtgcgctc ggcaccgcgt cggcgggttg
     4681 catcgccgat ttcatcgtgg tcaacatggt tgccgaggcc gcctccggtg ccgcaaccgt
     4741 caaggatgcg atgtccaggg cggagaagcg ggcaaagcgg tactacaagt aaggcgagag
     4801 gcgtgaggac tggggaaaag aatccagggg aagccggcgc atgaacaacg cgctgtcgcg
     4861 atttttcgat aaccgcaatg ttctcggtct gttgttcatg ctgcccgcag cagcactgct
     4921 gctggtgttc ctgacctatc cgctggggct gggtgtttgg ctgggtttca ccgacaccaa
     4981 ggtcggacgt gccggcggtt ggataggcat cgagaactac gagttcctgg tgaccgacag
     5041 cgtcttcacg ctgtcggtgt tcaatacctt cgtgtacacg gtggtggcga gcgtcatcaa
     5101 gttcattctt ggactgtggc tggccttgct gctcaacaag cacattccat tcaaatcgtt
     5161 cgtccgcgcc atcgtgctgc tgccgttcat cgtgcccacg gtgctctccg cgatcgcgtt
     5221 ctggtggatc tacgatgccc agttctcgat cgtgagctgg gtgttgatga aggtcgggat
     5281 catcgaccag tacataaatt ttctcggcga gccgaacctc gcgcgcatgt cgacgatcat
     5341 tgccaacatc tggcgtgggg tgcctttcgt ggcgatctgc ctgctcgccg gtctgcagac
     5401 cataccgccg tcgatgtacg aagcagcgag tatcgacggg gcgagcccct ggcagcagtt
     5461 ctggcatgtc acgctgccca tgctgtcgcc gatcatcgca gtggtcatga ctttctcggt
     5521 cttgttcacc tttaccgatt tccagttgat ctgggtgctg acccgcggcg ggccgatcaa
     5581 tgccacacat ttgatggcca cgctttcatt tcagcgggcc atccccggtg gggcattggg
     5641 tgaaggcgcg gcgattgcaa ccgcgatgat tccgttcctg ctggcggcga tcctgttctc
     5701 gtacttcggg ttgcagagga gacggtggca gcagggagga agcgacaaat gataagggcc
     5761 gagagacgag ggacgagggg cgagggtggg caagagcttt ttctaaaggg ctggtacccc
     5821 cttgcccctc ttccctcgcc cctctcccct cgctcagcga gcgcccatgg caagcactga
     5881 gccccgcgaa ggcatgcaat acctggagtc gctgccgcgg cgctgggtga cggtttacat
     5941 cccgttgctc gttttcctgt tcgtgctgct gtttccgttc tactggatgg tgatcacgtc
     6001 gttcaagccg aacgcggaac tgctgtccag cgccggcaac cctttctggg tcatcaaccc
     6061 gacgctggag cacttcagga aactcttctt cgacacctcc tatccggagt ggatgtggaa
     6121 tacggtgctg gtatcggtgg tatccacatt cgtttcactg gtcgccagcg tgttcgccgc
     6181 ctacgccatc gagcgtctgc gtttcagggg atcgcggcag accgggctgg cgatttttct
     6241 cgcctacctg attccgcctt ccatcctgtt tattccgctg gcaaccatgg tgttccagct
     6301 cgggatattc gacacgcgct gggcgctgat cctgacttat ccgacattcc tgatcccgtt
     6361 ctgtacctgg ctgctgatgg gctacttccg ctcgatcccc ttcgaactgg aggagtgcgc
     6421 actgatcgac ggcgccacgc gctggcagat cctcaccaag atcgtactgc cgctggcggt
     6481 gccgggactg atatcggcgg gcatctttgc gttcacgctg tcctggaacg agttcatcta
     6541 tgcgctcaca ttcatttcgg cttccgagaa caagaccgtc ccggtggggg tgatcaccga
     6601 actggtcgag ggcgacgtgt atcactgggg ctcgctgatg gcgggcgcgc tggtcggctc
     6661 gctcccggtg gccgtgatgt attcattctt tgtcgagtat tacgtcgccg gcatgaccgg
     6721 cgctgtcaag gagtaattct caccacagag acacagaggc acggagaaaa actggggcaa
     6781 tacaaagaaa tcttcggagt acgaagactt cccgagcggt cggttcttta atcttgttga
     6841 aattttgtct tccctgcggt cactactcca tcgtatttct ctgtgcctct gtggtggatt
     6901 ttttgaatct ctttttggtt agggagagga catggctgca aggagaaagc tgcgtagtta
     6961 cgagtggttc ggcggacccg ggatcgacag catggtgcac cggtcctgga tgaagaatca
     7021 gggcctgccg cgacacttgt tcgaaggccg tccggtgatc ggcatctgca atacctggtc
     7081 cgaactcacg ccctgcaatg cacatttccg cgtgatcgcc gatcgcgtca agcgcggcgt
     7141 ctacgaggcc ggaggcttcc cgctcgaatt cccggtgatg tcgctgggcg agccagtgat
     7201 gcgccccacc gcgatgcttt tccgcaacct ggcgtcgatg gatgtcgagg aatcgatccg
     7261 cgccaatccg ctcgacggcg tggtgctgct gaccggctgt gacaagacca cgccggcact
     7321 gatgatgggc gcggcatcct gcgatgtgcc gaccgtcgta gtgtccggcg gcccgatgct
     7381 caatggacgc tggcgcgatg agaccgtcgg ttccggcacg cacgtgtgga aattcaagga
     7441 aatgctcaag cgcggcgaga tgaccgacga ggatctgctg gatgccgagc aggccatgtc
     7501 gcgttcggcc ggtcattgca tgaccatggg caccgcttcg acgatggcat cgatggccga
     7561 ggcgctcggc atggcgctgc cgaccaatgc tgcgattccc gcagtggatt cgcgccgcta
     7621 cgtgctcgcg cacatggccg ggcagcggat cgtgcagatg gtgaaggaag gcctgcgcat
     7681 gtcgaagatc ctgaccaaac aggccttcga gaatgcgatc cgtgtcaacg gcgccatcgg
     7741 cggctcgacc aacgcggtgg tgcatctgct ggcgctggcc ggccgcatcg gcgtcaagct
     7801 cacgctggat gactgggatc gcctcggccg cgacctaccc tgcctggtaa acctgatgcc
     7861 ctccggcgag tacctcatgg aagacttcta ttacgccggc ggcctgccgg ttgtgatcaa
     7921 ggaactgggc agggccggcc tgctgcaccg gaaggcgcca accgtgaacg gccagagcat
     7981 ctgggaaaac gtcaaggacg ccaagaactg gaacgcgaaa gtgatcttcc cggtggacaa
     8041 gccgttcaag aagcagggcg gcatagcggt actgcgcggc aacctcgtgc ctaggggcgc
     8101 ggtgatcaag ccctcggctg cgacgccaaa gctgatgaag catcgtggcc gcgccgtggt
     8161 gttcgagaac atcgaggact acaagaagcg catcgacgat ccgaagctga aggtggacga
     8221 aacctgcgtg ctggtgctca agaactgcgg gccgaaaggc tatccgggct ttccggaggt
     8281 cggaaacttt gtgctgccag ccaagatcct gaaaacgggc atcaccgaca tggtgcgaat
     8341 ctccgatgcg cgcatgagcg gcaccgcgta cggcaccgtg gtgctgcaca gcgccccgga
     8401 agcggcggca ggcgggacgc tggcgctggt gcaagacggc gacatgatcg agatcgatgt
     8461 gccgaagaga aaattgcacg tggacgtgtc ggatgcagag ctggctaggc ggcgcaagaa
     8521 atggggggcg cctaaactca tggcggaccg cggctacgtg aagatctacg tcgatcacgt
     8581 caatcaggcg gacgaaggcg tggatctcga tttcctggtg ggcggcagcg gcacgccgct
     8641 cctgcgcgaa tcgcactaga agcagggacg aggaacgagg ggcgacgaaa ggaaaaaggg
     8701 gaacgataga agagctaaag agggatacat accgaaaatc ggggcgctgc ggcgccgccc
     8761 ccggcgggtt acctccggag ctgccactcc cccaaaccgg gcgcaccagc ataaaatgac
     8821 ggtttggact ggcgtggctc ccattttcct cttagccagc cagggc
//
LOCUS       DASQOP010000193         4601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2297564, whole genome shotgun sequence.
ACCESSION   DASQOP010000193 DASQOP010000000
VERSION     DASQOP010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2297564"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /gene="ptsP"
                     /locus_tag="VE030_07935"
     CDS             <1..1084
                     /gene="ptsP"
                     /locus_tag="VE030_07935"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004637088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HZF23380.1"
                     /translation="TLIRENEVLIVDGTNGVVIINPDRTVLGEYKLRQEQWELERLKL
                     RRLRKTRTTTLDGTAIELHANIELPDDVAGAKENGADGVGLFRTEFLYLNRGDLPTED
                     EQFEAYRKVVQDMDGLPVTIRTFDLGADKRIDNTERVAPNPALGLRAIRLCLAEPQMF
                     HHQLRAILRTSHYGKVRILIPMLSSAHELNQTLHLIDEAKKSLHDDGIAYDKKIKIGG
                     MIEIPAAALSLSTFTRKLDFLSIGTNDLIQYTLAIDRADDTVAHLYDPLHPAVLNLVA
                     HIIRSANRADVPVAVCGEMAGDVRLTRVLLGFGLRQFSMHPAYLLSVKQQVLKSDLQQ
                     VMGLTQKMLRSDDRDKLEQLLYRMNR"
     gene            1092..2153
                     /gene="holA"
                     /locus_tag="VE030_07940"
     CDS             1092..2153
                     /gene="holA"
                     /locus_tag="VE030_07940"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HZF23381.1"
                     /translation="MRIDSEQLAHHLTRGLKSLYVVFGEELLLALEAADRIRAAALEQ
                     GYDERRVLFVESGFDWGEVAMVGNSLSLFAPRRVLDLRIGSGKPGKDGSEALQRLAGS
                     LPEDTIVLITLPGLDRQQLASRWFDALERAGVAVHAAAVKREQLAQWLSGRLAQQDQR
                     ADVETLEFLVSRVEGNLMAAYQEVKKLALLFPAGTLPFEEVRDAVLDVARYNVFEIGA
                     TLLKGERAHFVRMLDGLCGEGAAPPLVLWAIAEEVRAMARVKAAVDAGQTLAQALREA
                     RVWGARQELMPRTLQRLTTIQLVASLRRAAEVDRMIKGLADGDVWDALLQLGLSLMTP
                     AQGGIPPGIRGKIPGTIRG"
     gene            2159..3415
                     /locus_tag="VE030_07945"
     CDS             2159..3415
                     /locus_tag="VE030_07945"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006413382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HZF23382.1"
                     /translation="MDIQSYMHSVGRAARAASREMAKADTRAKNDALLTMAQAVERNS
                     ARLLDANRKDVEAARARGLEAAMLDRLTLTPKSVASMAEGLRQIAALPDPVGEISGLN
                     YRPTGIQVGRMRVPLGVIGIIYEARPNVTADAAGLCLKSGNAAILRGGSEAIHSNQAI
                     GACVHEGLRKSLLPETAVQVIETTDRAAVGELITMKDYVDIIVPRGGKGLIERISSES
                     RIPVLKHLDGVCHVYIDDRADIDKAIRIADNAKTQRYGTCNTMETLLVHKNIAAAVLP
                     ALAKIYSDKGVELRGDQQARTLVSQMKPATEEDWYTEYLAPILAVRVVDDLDHAIEHI
                     AVYGSQHTDAIVTEDITRARRFLREVDSSSVLVNASTRFADGFEFGLGAEIGISTDKL
                     HARGPVGLEGLTSLKFIVLGDGHVRE"
     gene            3591..>4601
                     /locus_tag="VE030_07950"
     CDS             3591..>4601
                     /locus_tag="VE030_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZF23383.1"
                     /translation="MNHLIEIKVPDIGDFKNIPVIEVLVKPGDRINKEDALITLESDK
                     ATMEVPSPQSGVIREIKVKVGDKLSEGSVVLLLEGTSAEAPAAKAEAPKPAPAATVPK
                     APAAPAVTSSGPVTKGDIHSEVMVLGSGPGGYTAAFRAADLGKQVVLVERYPVLGGVC
                     LNVGCIPSKALLHAARVISEAEEMSPFGIAFGKPKIEIAKLRDWKDGVVDKSIRGLAG
                     LAKQRKVQVVQGIGKFTSPHMIEVKTPNGVKKVSFDYAIIAAGSSVARIPGFPYDDPR
                     LMDSTGALKLEDVPKRLLVIGGGIIGLEMATVYDAIGSKVTVVELLDGLIPGADRDLI
                     RPL"
BASE COUNT          930 a         1445 c         1444 g          782 t
ORIGIN      
        1 cacactgatc cgcgagaacg aagtgctgat cgtggacggc accaacggcg tggtcatcat
       61 caatcccgac aggaccgtgc tgggcgagta caaactgcgc caggaacagt gggagctcga
      121 acgcctgaaa ctcaggcggc ttagaaaaac caggaccacc acgctggacg gcaccgcgat
      181 cgaactgcac gccaacatcg agttgccgga cgacgtcgcc ggcgcgaagg agaacggcgc
      241 cgacggtgtc ggcctgttcc gcaccgagtt cctgtatctg aatcgcggcg acctgccgac
      301 cgaggacgaa cagttcgaag cctatcgcaa ggtggtgcag gacatggacg gcctgcccgt
      361 caccatccgc acctttgacc tgggcgcaga caagagaatt gacaacaccg aacgcgtcgc
      421 gcccaatccg gcgctgggtc tgcgcgcgat ccgcctgtgc ctggccgagc cgcaaatgtt
      481 ccatcatcag ctgcgtgcca tcctgcgcac ctcgcactac ggcaaggtgc gcatcctgat
      541 tccgatgctg tcctcggcgc acgagctgaa ccagactttg cacctgatcg acgaggccaa
      601 gaagagcctt cacgacgatg gcatcgccta cgacaagaag atcaagatcg gcggcatgat
      661 cgaaattccc gccgcggcgc tgtcgctctc cacgttcacc cgcaagctcg atttcctgtc
      721 gatcggcacc aacgacctga tccagtacac gcttgcgatc gaccgtgccg acgacacggt
      781 tgcgcacctc tacgacccgc tgcatccggc cgtgctgaac ctggtggcac acatcatccg
      841 cagcgccaat cgcgccgatg tgccggtcgc agtgtgcggc gagatggccg gcgacgtgcg
      901 cctcacaagg gtgctgctgg gtttcggact gcggcagttc tcgatgcatc cggcgtactt
      961 gctgtcggtg aagcagcagg tgttgaagtc cgacctgcag caagtcatgg ggctcaccca
     1021 gaagatgttg aggagcgacg atcgcgacaa gctggaacaa ctcctgtacc gcatgaaccg
     1081 ctaaaacaga aatgcggata gattcggagc aactggccca ccatctcacg cgcgggctga
     1141 aatccctcta tgtcgtattc ggcgaggagt tgttgctcgc gctcgaagct gccgatcgca
     1201 ttcgtgccgc ggcgctcgag cagggctacg acgaacgccg tgtactgttt gtcgaatccg
     1261 gattcgactg gggagaggtc gcgatggtcg gcaacagcct gtcgctgttc gcacccaggc
     1321 gcgtgctcga cctgcgtatt ggctccggca agcccggcaa ggacggatcc gaagccctgc
     1381 agcggctggc cggttcgctg cccgaggaca ccattgtgct gatcacgctg cccgggttgg
     1441 atcgccagca gctggcttcc agatggttcg acgcgctgga gcgcgccgga gtggccgtgc
     1501 atgccgccgc ggtcaagcgc gagcagctgg cgcagtggct atccggacgg ctggcacaac
     1561 aggatcaacg ggccgacgtc gaaactctcg aattcctggt aagccgggtc gaaggcaatc
     1621 tcatggcggc gtaccaggaa gtgaagaagc ttgccctgct ttttcccgcc ggcaccctgc
     1681 ctttcgaaga agtcagggac gcggtgctgg atgtagcgcg ctacaacgtt ttcgagatcg
     1741 gtgccaccct gctcaaaggc gagcgcgcgc acttcgtgcg catgctcgac ggcctctgcg
     1801 gtgagggcgc cgcaccgccg ctggtgctgt gggcgatcgc cgaggaagtg cgggcgatgg
     1861 caagggtaaa agcagcggtg gacgcggggc aaacgctggc gcaggcgctg cgggaggcgc
     1921 gcgtgtgggg cgcgcgccag gaactgatgc cacgcacgct gcagcgactg actacgatcc
     1981 agctcgtcgc ctcgctgcgg cgggccgccg aggtcgaccg catgatcaag gggctcgccg
     2041 acggcgacgt atgggacgcg ctgctgcagc tcggattgag cctgatgacg ccggcgcaag
     2101 gcggcatccc accgggaatt aggggtaaaa tccccggcac aattcgaggt tagtactcat
     2161 ggacattcag tcctacatgc actcggtcgg acgcgccgcg cgcgctgcct cgcgcgagat
     2221 ggcgaaggcc gacaccaggg caaagaacga tgcattgctc accatggcgc aggccgtcga
     2281 gcgtaacagc gctcgtctgc tggatgcgaa caggaaggat gtcgaggctg cgcgcgccag
     2341 gggactcgaa gccgcgatgc tggatcggct gacgctgaca ccgaagagcg ttgcatccat
     2401 ggccgagggg ttgcgccaga tcgccgcgtt gcccgatccg gtcggcgaaa tctccggact
     2461 gaactaccgg cccaccggca tccaggtggg gagaatgcgc gtgccgctcg gcgtcatcgg
     2521 catcatctac gaagcgcgtc ccaacgtcac cgcggacgcc gccgggttgt gcctgaagtc
     2581 cggcaacgcc gcaatcctgc gcggcggctc cgaggcgatc cattccaacc aggcgatcgg
     2641 cgcctgcgtc cacgagggat tgcgcaagtc gctactgccg gaaaccgcgg tccaggtgat
     2701 cgaaaccacg gaccgtgccg cggtcggtga actaatcacc atgaaagact acgtggacat
     2761 catcgtgccg cgcggcggca agggcctgat cgaacgcatc tccagcgagt cgcgcattcc
     2821 cgtgctcaag catctcgacg gcgtctgcca cgtgtacatc gacgatcgag ccgacatcga
     2881 caaagcgatc cgcatcgccg acaacgccaa gacgcagcgc tacggcacct gcaacaccat
     2941 ggaaacgctg ctggtgcaca agaacatcgc cgcagccgtg ctgccggcgc tggcgaagat
     3001 ttactccgac aaaggcgtgg aactgcgcgg cgaccagcag gcgcgcacgc tggtgtcgca
     3061 gatgaaaccc gcgaccgagg aagactggta caccgagtac ctagccccca tcctcgccgt
     3121 gcgcgtggtg gatgacctag atcatgcgat cgaacatatc gccgtttacg gttcgcagca
     3181 caccgacgcc atcgtcaccg aagacatcac ccgggcgcgg cgcttcctgc gcgaagtgga
     3241 ttccagttcc gtgctcgtca acgcgtccac ccgctttgcc gatggtttcg agttcggcct
     3301 gggtgcggag atcggcattt ccaccgacaa gctgcatgcc cgcggccccg tgggcctgga
     3361 aggcctgacg tcgctcaagt tcatcgtcct cggtgacggc cacgttcgtg agtaatctct
     3421 cgccgcggtc tggctaggaa ccatagaaca gaaaatctca ccacgaagac acgaaggttc
     3481 acgaagaagg gcaaaccccg gattttgtat ttctttctgt tacttcgtgg cttcgtggta
     3541 agaatttacg cacttgcttc tgctttgacg gttaatcacc aatagtcctc atgaaccatc
     3601 tgatcgaaat caaggtcccc gacatcggcg atttcaagaa cattccagtg atcgaagtgc
     3661 tggtcaaacc cggcgaccgg atcaacaagg aagacgcgct gatcactctg gaatccgaca
     3721 aggcgaccat ggaagtaccc tcgccgcaat ccggcgtgat cagggaaatc aaggtcaaag
     3781 tcggcgacaa gctttcggaa ggcagcgtgg tgctgctcct tgaaggcacc agtgccgagg
     3841 caccggcggc gaaggctgag gcacccaagc ccgcacccgc cgcaaccgtg ccaaaggcgc
     3901 ctgccgctcc tgcggtcacg tcatccggac cggtcacgaa aggcgacatt cattccgaag
     3961 tgatggtcct gggttccggc cccggcggct acaccgccgc gttccgcgcc gccgatctcg
     4021 gcaagcaggt ggtgctggtc gagcgttacc cggtactggg cggtgtgtgc ctgaacgtcg
     4081 gctgcattcc gtcgaaagca ttgctgcacg cggcgcgtgt catctctgaa gccgaggaaa
     4141 tgagtccctt cggcatcgca ttcggcaagc ccaaaatcga gatcgccaaa ctgcgcgact
     4201 ggaaggacgg cgtggtggac aagtcgatcc gcggtctcgc cgggcttgcc aagcaacgca
     4261 aggtccaggt ggtgcagggt ataggcaagt tcacctctcc ccacatgatc gaagtcaaga
     4321 caccgaacgg cgtgaagaaa gtgtcgttcg actacgctat catcgccgcc ggttcgagcg
     4381 tcgcgcgcat tcccggtttc ccgtacgacg atccgcggct gatggattcg accggcgcgc
     4441 tcaaactcga ggacgtgccc aagcggctgc tggtgatcgg cggcggcatc atcggcctgg
     4501 agatggccac ggtctacgac gccatcggct cgaaggtcac ggtggtggaa ttgctcgacg
     4561 gcctgatccc gggcgccgac cgagacctga tcaggccgtt g
//
LOCUS       DASQOP010000194         4795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_280375, whole genome shotgun sequence.
ACCESSION   DASQOP010000194 DASQOP010000000
VERSION     DASQOP010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280375"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            471..1802
                     /locus_tag="VE030_07955"
     CDS             471..1802
                     /locus_tag="VE030_07955"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZF23384.1"
                     /translation="MEFNILQHFKGLDELPKDPFNGNETGTSLFLTGGMGPKISQKMD
                     GYAKKAGMSRRNFIKSSLGFSAAMLAANEITGMKFFEVGAAEAADSGAKNEAIQVARA
                     SSDFIVDVHTHVCTRPGHYQLGVNTTERGMWFVQLLDDLGKAFGLPNGTRDMNVENYG
                     KLILQESDTTVGIFNPFGFREHYGGQDMIPMEYQVGVRNNWPKSTLMLAGGLTPNQGL
                     SVTLDRLTMYVEKYKISGLKLYTFDATAKKGWWFDDEKLAYPIWEKCRKLGIKNIGCH
                     KGIPFGQFYARYAHAEDFDAAADDFLDLNFIAYHSAWPYHNELAALKTFKPQRKNLYC
                     EVGSTFAATVTSRPLECAHVIGTLLRDLGPDYVLWGTDSLLWGNPQWQIEAFRKFQIP
                     DQLVEGHGYPKITDEIRRKVFGENAARIWNIDKQAAMQAKAEILSNKTSFA"
     gene            1884..2171
                     /locus_tag="VE030_07960"
     CDS             1884..2171
                     /locus_tag="VE030_07960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZF23385.1"
                     /translation="MPTYEYACDPCRVIYQTRHGIKDPRPEHCPKCKGELRKVLSAPS
                     LNTRNHSSPTQAKYAKLSESDEIAKEKDLQKVYETVWIPPEVKHNPWDHDH"
     gene            2322..3701
                     /locus_tag="VE030_07965"
     CDS             2322..3701
                     /locus_tag="VE030_07965"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HZF23386.1"
                     /translation="MGATWLACGSSEVLDPAALTAGSFTSPEAGANAFSIPIAMLDRE
                     QAAQFAKGREQFHEAWVVAPDPSGVWGLGPTFNEDRCAHCHENNGRARAPAQRQEAVR
                     GMLVRLSLPGQGPHGEPRPHPAYGDQLQNRGIPGRVPAEGQAIFSYTKREVGFSDGET
                     VTLRVPKIEFEGLQFGELGADIMISPRIAPAMVGLGLLEAVPEQSILAIAREQEKLGV
                     SGKPNYVWDYENSRTVLGRFGWKAGQPNLRQQTAVAFLNDIGATSHFFPEENCPSVQV
                     QCLDVPSASKCGGQGGCTGNYRPEVVPSRLSNITLYLQALAVPARRNVNDPDVKRGEQ
                     LFFQSRCSACHVPELRTGGKTAIPAAADLPIHAYTDLLLHDMGEGLADGRPEYRADGR
                     EWRTPPLWGIGLVPEVNGHGDLLHDGRARNIAEAILWHGGQAEPSRDMFRALPKADRE
                     ALVKFVSSL"
     gene            3725..4507
                     /locus_tag="VE030_07970"
     CDS             3725..4507
                     /locus_tag="VE030_07970"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZF23387.1"
                     /translation="MAIHEDDGAVTAVSSPAYADRELREFNRRFYDSLWTDARLVEPE
                     RFNTWPLVRSLVAQARCRLEVAPGLRPRLPIEGTHFVDISGPALAKLRGRAASVTLDS
                     ISELPFADSVFDLVCAFDIVEHVDDDDLALSELSRVCAPGGAFLLSVPLHTSRWTVFD
                     EFVGHRRRYEPARLLVQLREHGFAVESSAVYGMQPQSSWMLDLGMWWLTHRREQAMWW
                     YNRVMMPLGVRFQKKLALCPGMIDTSKVDEILLVCRKQGSAQ"
     gene            complement(4514..>4795)
                     /locus_tag="VE030_07975"
     CDS             complement(4514..>4795)
                     /locus_tag="VE030_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdenumtransferase MoeA"
                     /protein_id="HZF23388.1"
                     /translation="LLYLQGQATVAPLPTLKVTCTSAIKKAPGRTEFQRGVLSRAADG
                     QWTVRTTGDQGSGILSSMSQADCFIVLPTEAGNLPAGAVVEVQLLEGLI"
BASE COUNT          953 a         1461 c         1500 g          881 t
ORIGIN      
        1 gtaggcgtcg gcattctgcc ggctggtcca tccgtgccag attctgctga tcatgggtga
       61 ttccatcagt cgtaggttca aggttggcag atagatcgaa tcctaatgcg atccgcgtcg
      121 tcgccggtgc tgcgctgccg catcacaaag gccgtgtttc ccactgccct ttgaacgagt
      181 ccctcgggca gcctccagcc ccgcgtgcta cgccgaagcg tttgtttttc cgtgctttca
      241 aagtgccagg aacgattgaa ggaatctgtc caccctcact aaccgttgtc cgggaaacgt
      301 ctggccataa agtgaccggg caagcgttgc aacgcgcgtg cgagaagagt agcgtgaccg
      361 aaaacgagga ttgcattatc ggtgtcggaa tgagcggccg gcccggtttt tctggcgtgc
      421 cgttccgtgg tcaaaacaga aattttgata acaaacggag gaggagcgcg atggagttca
      481 atatcctgca gcacttcaag ggccttgacg agcttcccaa ggacccgttc aacggtaacg
      541 aaaccggcac ttcattgttc ctcaccgggg gcatgggtcc gaagatctcc cagaagatgg
      601 acggctatgc caagaaggcc ggcatgtccc gacgcaattt catcaaatca tccctgggtt
      661 tctcggcggc gatgctcgct gccaacgaaa tcacgggaat gaagttcttc gaagtcggcg
      721 cggccgaagc tgccgactct ggcgccaaga acgaagccat ccaggtcgct cgcgccagct
      781 ccgacttcat cgtcgacgtt cacacgcacg tctgtacccg cccgggccac tatcaactcg
      841 gcgtcaacac caccgaacgt ggcatgtggt tcgtgcagtt gctggacgac ctcggcaagg
      901 ccttcggcct gcctaacggc acacgcgaca tgaacgtcga gaactacgga aagctgatcc
      961 tgcaggaaag cgacaccacc gtcggcatct tcaacccgtt cggcttccgc gagcattacg
     1021 gcggccagga catgatcccg atggagtacc aggtcggggt gcgcaacaac tggccgaagt
     1081 ccaccctgat gctggctggc gggctgacgc cgaaccaggg cctgagcgtg acgctggatc
     1141 gcctgaccat gtacgtcgag aagtacaaga tctcgggcct caagctctac accttcgacg
     1201 cgactgccaa gaagggctgg tggttcgacg acgagaaact ggcgtacccg atctgggaga
     1261 agtgccgtaa gctgggtatc aagaacatcg gctgccacaa gggcatcccg ttcggccagt
     1321 tctacgcacg ctatgcgcac gccgaggatt tcgacgccgc ggcggatgac ttcctggatc
     1381 tgaacttcat cgcctatcac tcggcctggc cgtatcacaa tgagctggcg gcgctgaaga
     1441 ccttcaagcc gcagcgcaag aacctgtact gcgaagtcgg ctcgacgttc gccgcgacgg
     1501 tcaccagccg gccgctggag tgcgcgcacg tgatcggcac cctgttgcgc gacctcggtc
     1561 cggattacgt gctgtggggc accgactcgt tgctgtgggg caatccgcaa tggcagatcg
     1621 aggcgttccg caagttccag atccccgatc aactggtcga gggtcatggt tatccgaaga
     1681 tcaccgatga aatccgccgc aaggtctttg gcgagaatgc ggcgcgcatc tggaacatcg
     1741 acaagcaggc tgcgatgcag gcgaaagccg aaatactgtc caacaagacc agcttcgcat
     1801 aaaacaacgc gggtatcatc gatggggccg gttccaggtg gagtcggccc cattaatttg
     1861 agaacagcgg ccggagacac gaagtgccaa catacgaata cgcctgcgac ccttgtcgcg
     1921 tcatttacca gacgcgtcac gggatcaagg acccgcgccc ggagcattgc cccaagtgca
     1981 aaggcgaact gcgcaaggtt ctgtcggcgc ccagccttaa tacccgcaac cattccagtc
     2041 cgacgcaagc caagtacgcg aagctgtcgg aaagcgacga aatcgccaag gagaaggacc
     2101 tccagaaggt ctatgaaacc gtctggatcc cgccggaagt gaagcacaat ccgtgggacc
     2161 acgaccactg atcgccgcgc agttcatgcg cttgatcacg cccaaagcag ccaggccgcg
     2221 tgcagaggaa gcctcgcgtg tttcctgagt ccgctcgagg gggacctgcc cggaaacgga
     2281 ctgccgtcag cgcgacaagc ctgcttgcat cggcctgcct gttgggggcg acatggctgg
     2341 cgtgcggaag ctccgaagtt ctggatcctg ccgcgctcac cgcgggcagt ttcacctctc
     2401 ccgaggccgg cgccaacgcc ttttccattc ccatcgccat gctcgacagg gaacaggctg
     2461 cgcagttcgc caaaggcagg gagcagttcc acgaagcctg ggtggttgct cccgatcctt
     2521 ccggtgtgtg gggactgggg ccgactttca acgaggatcg ctgcgcgcat tgccatgaaa
     2581 acaacggccg cgctcgggcg ccggcacaac ggcaagaggc tgtgcgcgga atgctcgtgc
     2641 gcttgagcct gcccggccag ggaccgcacg gggagccccg gccgcacccg gcgtacggag
     2701 accagttgca aaaccgcggc attcccggcc gcgtgccagc cgaaggtcag gcgattttca
     2761 gctacacgaa acgcgaggtc ggcttttccg acggcgaaac tgtgacgctg cgggtgccga
     2821 agatcgagtt cgaggggctg cagtttggcg agctcggagc cgacatcatg atttcgccgc
     2881 gcatcgcgcc ggccatggta gggctgggat tactggaagc ggtgcccgaa caatcgatac
     2941 ttgccatcgc cagggaacag gaaaagctcg gcgtgagcgg caagccgaac tacgtttggg
     3001 attacgagaa cagcaggacc gtgctcggac gcttcggctg gaaggcgggg cagcccaatc
     3061 tgcgccagca gacagcggtg gcgtttctga acgacatcgg cgcgacctct cacttttttc
     3121 cggaggagaa ttgtccgagt gtgcaggtgc aatgcctgga cgtgccgtcc gcaagcaagt
     3181 gcggcgggca gggcggatgt accggaaact atcggccgga ggtcgtcccg agccgcctga
     3241 gcaacatcac gctatacctg caggcgctcg cggttccagc ccggcgcaat gtaaacgacc
     3301 ctgatgtgaa gcggggcgaa cagctgttct tccaatccag gtgcagtgcc tgccatgtgc
     3361 cggaactcag gaccggtggc aaaacagcga tccctgccgc ggcggacctg ccgatccacg
     3421 cctacaccga cctgttgctg cacgacatgg gtgaggggct cgctgacggt cgccccgaat
     3481 accgggccga cggccgcgaa tggcgcacgc ccccgctctg ggggatcggg ttggtgcccg
     3541 aggtaaacgg ccacggcgac ctgctgcatg acggccgtgc acgcaatatc gccgaagcga
     3601 ttctgtggca cggcggacag gcagagccgt cgcgggatat gtttcgcgca ctgcctaaag
     3661 ccgatcgcga agccttggtg aagttcgtca gttccctctg acgcaggcgt gcacgaggcc
     3721 aatagtggct atccatgagg atgacggtgc ggtgaccgcg gtatcttcgc cggcgtacgc
     3781 ggaccgggaa cttcgtgaat ttaatcgcag gttctatgat tcattgtgga cggacgcgcg
     3841 tctggtcgag cccgagcgct tcaatacctg gccgctggtg cgctctctcg ttgcgcaggc
     3901 gcggtgccgg ctcgaagtcg ctccgggcct gcgcccgcgg cttccgatcg agggaacgca
     3961 cttcgtggac atcagcgggc cggcgcttgc gaaactgcgc ggacgcgcgg cgagcgtcac
     4021 gctcgactcg atttccgagc ttccgtttgc cgactccgtg ttcgaccttg tgtgcgcctt
     4081 cgacatcgtc gagcacgtcg acgatgacga cctcgcgctg tcggagctat cccgcgtttg
     4141 cgcacccggc ggggcatttc ttctttccgt ccctctgcat acgtcgcgtt ggacggtgtt
     4201 cgatgaattc gtggggcacc ggcgccgtta tgagccggcc cgtctgcttg tccagctcag
     4261 ggagcacggc ttcgcggtgg aaagcagcgc cgtttacgga atgcagccac agtcctcgtg
     4321 gatgctcgat cttggcatgt ggtggctcac gcaccggcgt gagcaagcga tgtggtggta
     4381 caaccgtgta atgatgccgc ttggggtgcg cttccagaag aaactggccc tctgtccggg
     4441 gatgatcgat accagcaaag tcgacgagat tctgctggtg tgccgcaaac aaggctctgc
     4501 acagtgaacc tttctagatc aaaccttcga gcaactgaac ttccaccacg gcgccggcgg
     4561 gcaagttgcc cgcctcggtc ggcagaacga tgaagcagtc ggcctggctc atcgacgaaa
     4621 gaattcccga accctggtct ccagtggtgc gcaccgtcca ttgcccgtcc gcagcgcggc
     4681 tgaggacgcc gcgctggaat tcggtgcgcc cgggcgcttt cttgatcgcg gacgtgcacg
     4741 tcaccttgag ggtcggcagc ggcgcaaccg tggcctgtcc ctgcaggtag agcag
//
LOCUS       DASQOP010000195         6374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_84796, whole genome shotgun sequence.
ACCESSION   DASQOP010000195 DASQOP010000000
VERSION     DASQOP010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84796"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1819
                     /locus_tag="VE030_07980"
     CDS             <1..1819
                     /locus_tag="VE030_07980"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HZF23389.1"
                     /translation="VDVLMPEPDRMSPVNLAGLIERLDAGLARRLAQLPDNDAVLAES
                     LRRYPVALGIAGVEQAEAGAAATGRTTPFRVHGPDPTPHLRQFAGTLRSLPQLDAAAR
                     GHGLLSVDPRGGVVHRVPLVASVSGVLTPALSIELLRIASDLPAFSIAADGGGARSIG
                     VGDTVIPANRDGTVWVHFGAHDEARFVSALDAIRGDIDPELIRGKIALVGATGLGLLD
                     HQTTPLGERIPGIEIHAQILENIYEDSLLRRPRHFDHTERLLLLGCGLILILAVPAAR
                     ARMAALIYGTLVAGLLMLGFALYYWKQLLLDVAWPTTAASVLFGVMLTGALAEADRQR
                     RALRRALEAERDAAARAAGELEAARRIQMGMLPPPSADFYGDPRFSLQALIETAKSVG
                     GDLYDFFKLDADRLFFMVGDVAGKGLPASIFMAVSKALYKSAALRSRLEGAAVISEVM
                     RAANGEIARDNPEMLFVTVFAGILDLKSGELSYCNAGHDAPCLLHAQRPAPLRLESDS
                     GPPLCVIEGYEYQSERYRMTPGESLCLYTDGVTEAMNAGGELYGRQRLFSVLTAVLAA
                     GDAVKLVDAVRDDVREFVSASERSDDLTILALRWNGGSL"
     gene            complement(1824..2300)
                     /locus_tag="VE030_07985"
     CDS             complement(1824..2300)
                     /locus_tag="VE030_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HZF23390.1"
                     /translation="MSKVLMLQREFLMFSFLLLLCALVALPSLAAEAGRVKVSKGEVQ
                     IERDGKRLPAPVGATIQARDIVKTGPDGSVGITFQDNSLLSAGPNSELVIERFVFDST
                     THKGEFDTALRKGTLAVVSGKIVKQSPEAMRIKTPAAIMGVRGTEFVVKVDESATK"
     gene            2418..2864
                     /locus_tag="VE030_07990"
     CDS             2418..2864
                     /locus_tag="VE030_07990"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZF23391.1"
                     /translation="MPVHSRNSVKRLSRTFPAQLSAFEQLKALIEEFGDAAGLGREDR
                     HKLTLIVEELFTNTVTHGHRGDSNAPVQVILEDTAAGIHLTYEDTAPQYDPLAASRRT
                     DIESTINERRVGGLGVFLTIGLANGADYRFIEGRNRISLTLVPSRE"
     gene            complement(2839..5445)
                     /locus_tag="VE030_07995"
     CDS             complement(2839..5445)
                     /locus_tag="VE030_07995"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZF23392.1"
                     /translation="MAISIQHSWPLLLLLAIPLLWWGARRTRTNLGRRHLAVVTVLRS
                     LAVAVLALALMRPQWHAGSNDVAVVYALDISRSVSSSFLYSALAWIGNADREGKPARA
                     RYLAFADRPVLLEKLEQVRNLAVTEGRPREDAIDQAATNLERALDEALLGLDRDRVQR
                     LVLFTDGNQTQGDVWRVLARLKEANVRVFPIPAKVRDDRDAWLESIDVPQGIRQGEPV
                     TVTVRAFSPGEARARVVLRTGNTVLGDRAVRLTAGLNRVSFEIQLSSAGSNTLAAELI
                     AEGDRVPDNNRVQQSAWVAKAPRVLYVEGQPGAAGYLHDALARQGIEVSVAAPVDIPE
                     SAAAFALYDAVILSDAPAKAIAPAKMRAIESYVRDGGGGLLFAAGENVYGEQGYSGTP
                     VEKVLPVQFRAQEKRKDLALVIALDRSYSMRGRKMELAKEATRAALDLLEEQHQFAVV
                     AFDSQAHISVPMQFVRAKRKAEDQIARIQASGQTNIYPALGIVYRLLQKTDAKAKHVI
                     LLSDGDTHPADFEGLVKRMADEKMIVSTVAIGEGADRELMSNIAKWGGGRAYSTVSPE
                     AVPQIFVEETQRAVRSNLLEDAFKPVVKRRMEAFRGVDFEQAPPLKGYVSATARDNAE
                     VFLVSQSGSPVLARWQYGLGKVVVFTSDVKNRWAVNWLDWPGYGKLWAQLTRETMRRD
                     SGEELDFRVSREDGEAVISLNALTADGHFRDDLAPRVRVTGPDGTAATAELHHSGPGA
                     YRLRVPLASATAARFELADSPGLSRRAVDRAGSRRLYHAYPDEYRALPPNLELLRTLA
                     EETGGKLAPSIAEVFAPNGDHSRVSHMLWPWLALLALLLYLLDIAVRRAPLAWRRLGD
                     DAAPATRGSAPG"
     gene            complement(5448..>6374)
                     /locus_tag="VE030_08000"
     CDS             complement(5448..>6374)
                     /locus_tag="VE030_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23393.1"
                     /translation="DGLEADDVAYALLPMRRVVRVGLVSNGNPYLEKSLHAQPRVRLV
                     AISPRGFTERRDIDLWVFDRFAPRTQPGTPALLFRPNPVPWLPAPGKESTDVTVAAWD
                     GAHPLLENLSLRDLVVERAVAARPASGSRERDVVLVSARGHVPLVVAHEGAARWVWFA
                     FGLEDSNFALHAEFPIFLGNALVWLAGEHGAFAAGLGVVEVPVAKARVVAPDGIELPA
                     QAIPGGTVFEVTEPGMFTAVSASQRLRVVANLLDRRVTDVNRSPLAPLQSLAVESTQA
                     PRLPLDSWAALLLAAVLLLVFEWWSWNRRATV"
BASE COUNT         1117 a         2188 c         1986 g         1083 t
ORIGIN      
        1 cgtggacgtg ctgatgccgg aacccgaccg catgtcgccc gtcaacctag cggggctgat
       61 cgagcgcctg gacgccggac tcgcccgccg cctggcacag ttgcccgaca atgacgccgt
      121 ccttgcggaa tcgctgcgcc gttacccagt ggctctcggg atcgccggcg tagaacaggc
      181 agaagcgggc gccgcggcaa ccggccgcac taccccgttt cgcgttcacg gcccggaccc
      241 cacgccgcac ctgcggcagt ttgccgggac actgcgcagc ctcccccaac tcgatgccgc
      301 cgcacgcggc catggcctgc tgagcgtgga tccgcgcggc ggagtggtgc accgcgtgcc
      361 gctggtcgca tcggtatccg gcgtgctgac tccggcactg tcgatagagc tgctgcgaat
      421 agccagcgac ctgccggcgt tctcgattgc ggcggacggc ggcggggctc ggagcatcgg
      481 cgtcggagac accgtgattc ccgccaatcg cgacggcacg gtatgggtgc acttcggtgc
      541 ccacgacgag gcccgcttcg tatcggcgct cgacgcaatc cgtggcgaca tagatcccga
      601 actcatccgc ggcaagattg cgctggtcgg cgcaaccgga ctgggattgc tcgaccacca
      661 gaccacgccc ctgggcgaac gcataccggg aatcgagatt cacgcgcaga tcctcgagaa
      721 catatatgag gacagcctgc tgcgccggcc ccgccatttc gatcacacgg agcgcttgct
      781 gctgctcggc tgcgggctga tcctgatact cgcagtcccg gcagcaagag cgcggatggc
      841 cgcgctgatt tacgggaccc tcgtcgccgg gctgctcatg ctcggcttcg ccctgtacta
      901 ttggaagcaa ttgctgctcg acgttgcctg gccaacgacc gcggcgagcg tccttttcgg
      961 cgtcatgctg accggggcgc tggccgaggc cgaccgccag cgccgtgcgc tcaggcgagc
     1021 gctggaagcc gaacgcgacg cggcggctcg cgctgccggc gaactggaag cggcacgacg
     1081 catccagatg gggatgctgc cgccaccatc ggcagatttc tacggcgatc cgcgcttttc
     1141 actgcaggcg ctgatcgaaa cggcgaaatc ggtcggcggc gatctctacg atttcttcaa
     1201 gctcgacgcc gaccggctct tcttcatggt cggtgacgtt gccggcaaag gcctgccggc
     1261 gagcatcttc atggcggtca gcaaggccct gtacaagagt gcggccttgc gcagccgcct
     1321 ggagggcgct gcggtcatca gcgaagtgat gcgggccgcc aatggcgaga ttgcgcgcga
     1381 caacccggag atgctgtttg ttaccgtatt cgccggcatc ctcgatctga aatccggtga
     1441 actcagctac tgcaatgccg ggcacgacgc cccttgcctg ctgcatgcgc aacgcccggc
     1501 gccgttacga ctggagagcg attcggggcc gccgttgtgc gtcatcgaag gctacgagta
     1561 ccagtccgag cgctaccgga tgacgccggg cgaatcactt tgcctttata ccgatggcgt
     1621 gaccgaagcc atgaatgcag gcggtgagct ctacgggcgc cagcggctgt tctcggtgct
     1681 tacagcggtg cttgccgccg gcgatgcggt aaagctggtg gatgcggtgc gcgatgacgt
     1741 gcgcgaattc gtcagcgcca gcgagcgctc ggacgatctc accatcctgg cgctgcgctg
     1801 gaacggcggc agcctatgag aaattactta gtggccgatt cgtcaacttt gacgacgaac
     1861 tcggtgccgc gcacgcccat gattgctgct ggcgttttaa tccgcatcgc ttcaggcgac
     1921 tgcttgacga tcttgccgga gaccaccgcg agcgtgccct tcctcagcgc cgtatcgaac
     1981 tcgcccttgt gggtggtact gtcgaacacg aagcgctcga tcaccagttc gctgttgggg
     2041 cccgccgaca acaggctgtt gtcttggaag gtgatgccca ccgagccatc ggggccggtc
     2101 tttacgatat cgcgcgcctg gatcgtggca ccgaccggcg cgggaaggcg cttgccgtcg
     2161 cgttcgatct gcacctcgcc cttcgacact ttgacgcgtc ccgcttccgc cgccagcgag
     2221 ggcaatgcga ccagtgcgca gagcagcagc agaaagctga acatcaggaa ttctctttgc
     2281 aacatcaata ccttgctcat ttgtctcccc ttcgtcggta aggcggcagg cagaggatgc
     2341 cggcgcacag attatcgagc aagaagcatg cacgttcccg tcgtaacaac gggtccggcc
     2401 cttgctggaa agtgcaaatg cccgtccatt ccaggaacag cgtgaagcgc ctgagccgca
     2461 cattccccgc ccaattgagc gctttcgagc agttgaaggc gctgatcgag gaattcggag
     2521 acgcggccgg actcggccgc gaggatcgcc acaaactgac gctaattgtc gaggaactgt
     2581 tcaccaatac cgtgacccac gggcaccgcg gggacagcaa tgcgccggtg caggtcatcc
     2641 tggaagatac cgcggccggc atacatctga cctacgaaga taccgcgccc cagtacgacc
     2701 ccctggccgc cagccggcgc accgacatcg agtccaccat caacgaacgc cgcgtcggcg
     2761 gcctcggcgt gttcctgacc atcggcctgg ccaacggagc ggactaccgt ttcatcgaag
     2821 gccgcaatcg catttcgctc accctggtgc cgagccgcga gtagcgggcg cggcatcatc
     2881 tcccagccgg cgccacgcca gcggtgcacg acgcaccgcg atatccagca gatacagcaa
     2941 caaagccagc agcgccagcc acggccacag catgtgcgaa acgcggctgt ggtcgccatt
     3001 cggcgcaaac acttcggcga tgctgggtgc cagcttgccc cccgtctcct cggccagcgt
     3061 gcgcagcagt tcgagattcg gcggcaatgc ccggtattcg tccgggtagg cgtgataaag
     3121 cctgcggctg cccgcgcggt ccaccgcccg ccgcgacagg cccggcgaat ccgccagttc
     3181 gaaacgcgcc gccgtggcac tcgccagcgg tacgcgcagc cggtacgcac ctggcccgct
     3241 gtggtgcagt tccgccgttg ctgccgtccc gtcgggcccg gtcacccgca cccgcggcgc
     3301 cagatcatcg cggaagtgcc cgtccgcggt gagcgcattc aggctgatca cggcttcgcc
     3361 gtcttcacgc gatacacgga aatcgagctc ttccccggaa tcgcggcgca tggtttcgcg
     3421 cgtaagctgc gcccagagct tgccgtaacc cggccaatcg agccagttca ccgcccagcg
     3481 attcttcacg tcggaagtaa acaccaccac tttgccgagg ccgtattgcc agcgcgccag
     3541 caccggcgat ccggattgcg agaccaggaa gacttcggcg ttgtcgcgcg ccgtggcgct
     3601 cacgtagccc ttcagcggcg gggcctgctc gaagtccacg ccgcggaaag cctccatgcg
     3661 gcgcttgacc accggcttga atgcatcttc gagcagattc gagcgcaccg cacgctgcgt
     3721 ttcttcgacg aaaatctgcg ggacagcttc gggactgacg gtggaatagg cgcgtccccc
     3781 gccccacttg gcgatattgc tcatcagttc gcggtcggcg ccttcgccga tcgccacggt
     3841 ggaaacgatc atcttttcat cggccatgcg cttgaccagc ccctcgaaat cggcagggtg
     3901 ggtatcgccg tcggacagca ggatcacatg cttggccttg gcatcggtct tttgcagcaa
     3961 ccggtagacg atgccgagag cgggatagat gttggtctgg ccgctcgcct ggatgcgggc
     4021 aatctggtct tcggccttgc gtttggcgcg cacgaactgc atcggcaccg aaatgtgcgc
     4081 ctgagagtcg aatgccacca ccgcgaactg gtgctgttcc tccagcagat cgagcgcggc
     4141 gcgggtggcc tccttcgcca gctccatctt gcgcccccgc atgctgtagg agcggtcgag
     4201 cgcaatcacc agcgcaaggt ccttgcgttt ctcctgggca cggaactgca ccggcagcac
     4261 tttctccacc ggcgtgccgc tgtagccctg ttcgccgtag acattctcgc cggccgcaaa
     4321 caacaatcca ccgccgccat cgcgcacgta ggactcgatc gcccgcattt tcgcgggcgc
     4381 gatcgccttt gccggcgcat cgctcaggat caccgcgtcg tagagcgcaa atgccgctgc
     4441 cgattcggga atgtcaacgg gtgctgcgac gctgacttcg atgccctggc gcgccagcgc
     4501 atcgtgcagg tatccggcgg ctccgggctg gccctcgaca tacagcaccc gcggcgcctt
     4561 ggcgacccat gcggactgct gcacacgatt gttgtcgggt acgcggtcgc cttcggcaat
     4621 gagttccgcc gccagcgtgt tgctgccggc gctggacagc tgaatctcga aggaaacgcg
     4681 attcagccct gccgtcagtc gcacggcgcg gtcgccgagc accgtattac cggtcctgag
     4741 caccacgcgc gcgcgcgcct cgcccggcga gaacgcacgc acggtcaccg ttaccggttc
     4801 gccttggcgt atgccttggg gcacgtcgat gctttccagc caggcgtcgc ggtcatcgcg
     4861 cactttcgcc gggatcggaa acacccggac gttggcttcc ttgagccgcg ccaacacgcg
     4921 ccagacatcg ccttgggtct ggttaccgtc agtaaacaac accaatcgct ggacgcgatc
     4981 gcggtccaag cccagcagcg cctcgtcgag agcgcgctcg aggttggtcg cagcctggtc
     5041 gatcgcgtct tcgcgcgggc ggccttcggt gaccgcgaga ttgcgcacct gttccagttt
     5101 ctccagcagc accggccgat cggcgaacgc gaggtagcgc gcccgcgccg gcttgccttc
     5161 gcgatcggca ttgccgatcc aggcgagcgc cgagtagagg aaggacgaag acacgctgcg
     5221 tgatatatcc agcgcataca ccactgcgac atcgttcgat cctgcgtgcc actgcggccg
     5281 catcagcgcc agcgccagca cggcaaccgc caggctgcgc aagacagtaa ccactgcgag
     5341 gtgcctgcgg ccgaggttgg tgcgggttct ccgcgcgccc caccacaaca gcggaatcgc
     5401 caggagcagc agcaacggcc agctgtgctg aatgctgatc gccatcgtca taccgttgcc
     5461 cgccggttcc agctccacca ctcgaatacc agcaatagca ccgccgccaa cagcaatgcc
     5521 gcccaggaat cgagcggcaa tcgcggcgcc tgcgtcgatt cgactgccag ggactgcagc
     5581 ggcgccagcg gcgaacggtt gacatcggtt acgcgccggt cgagcaggtt ggccaccact
     5641 cgcagccgct ggctggcgga tacggcggtg aacatacccg gttcggtgac ttcgaacaca
     5701 gtgccgccgg gtatggcctg cgccggcagc tcgatgccat ccggcgccac gactcgcgcc
     5761 tttgcgacgg gcacttcgac cacacccaat cctgctgcga aagcgccgtg ctcgcccgcg
     5821 agccaaacca gcgcgttgcc gaggaatatc ggaaattcgg cgtgcagtgc gaagttggaa
     5881 tcttccagtc cgaaagcgaa ccacacccag cgcgcagcac cctcgtgcgc caccaccagc
     5941 ggcacatggc cgcgcgcgct caccagcacc acgtcgcgct cgcggctgcc gcttgccggt
     6001 cgcgccgcca ccgcacgttc gacgacgaga tcgcgcagcg acaggttctc gagcagcggg
     6061 tgcgcgccat cccaggccgc caccgtgacg tcggtggact ccttgcccgg cgccggcaac
     6121 cacggcaccg gatttggccg gaacagcagc gccggcgtac ccggctgcgt ccttggagcg
     6181 aaccggtcga atacccataa gtcgatgtca cggcgctccg tgaagccccg cggcgaaatc
     6241 gccacgagcc gcactcgcgg ctgcgcatgc agggatttct cgaggtaggg attaccattg
     6301 ctcaccaggc ccactcgcac cacgcgccgc atcggcagca gtgcgtaagc gacgtcatcg
     6361 gcctccaggc cgtc
//
LOCUS       DASQOP010000196         9297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1092473, whole genome shotgun sequence.
ACCESSION   DASQOP010000196 DASQOP010000000
VERSION     DASQOP010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9297
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092473"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            339..1862
                     /locus_tag="VE030_08005"
     CDS             339..1862
                     /locus_tag="VE030_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HZF23394.1"
                     /translation="MLQAADLTIRLNPADDVVIARVELPASTEIIKEKIRTAVTIPAG
                     HKIATHDIGEGSPVRRYNQIIGFAVKPIKAGDHVHVHNLAMGDFQRDYAFCADYKPVD
                     YVANPATFQGIVREDGRVATRNYLGIISSVNCSAHVCRSIAKYFDEDFMAQYPNVDGV
                     VPIHHKTGCGMGSEGEPVELLRRVLAGYAVHQNFHSSLMIGLGCEANQINNLLASQGL
                     KRSKQLQAFTIQETGGTRKTVADGIARIKELLPDANNVKRETVPASHLTLGLQCGGSD
                     GYSGISANPALGAAVDILVRNGGSAILSETPEIYGAEHLLTRRARSREVGEKLVKRIK
                     WWEDYTTRLGGEMNNNPSPGNKAGGLTTILEKSLGAVAKGGTQALMDVYEYAERVTAK
                     GFVFMDTPGYDPVSATGQVAGGANMICFTTGRGSAYGCKPSPSLKLATNSAMFNKQEE
                     DMDINCGVIVDGKASIQEVGEQFFQMILRTASGEQSKSEAMGYGEDEFAPWVLGPTM"
     gene            2048..2347
                     /locus_tag="VE030_08010"
     CDS             2048..2347
                     /locus_tag="VE030_08010"
                     /inference="COORDINATES: protein motif:HMM:NF043076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhaM family polyhydroxyalkanoate granule
                     multifunctional regulatory protein"
                     /protein_id="HZF23395.1"
                     /translation="MAEDSSKELFEFFQKMWNPMSFPIPGMFQPTMSVEEVEKKIAEL
                     QSVENWLKMNLTFVQMTMKTMEMQKSALQSIQESAAATSQKAPKKPKDEGRGTKT"
     gene            2713..2919
                     /locus_tag="VE030_08015"
     CDS             2713..2919
                     /locus_tag="VE030_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23396.1"
                     /translation="MISAFRRLFNTRRPQISVRSRVNLLGTFGLLERTDGVVESIIGD
                     EAYVEWANGARSVESTRRLVRISA"
     gene            complement(3121..4113)
                     /gene="pip"
                     /locus_tag="VE030_08020"
     CDS             complement(3121..4113)
                     /gene="pip"
                     /locus_tag="VE030_08020"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HZF23397.1"
                     /translation="MDYAAPGIRTELYPDIEPHQSGYLRLDQRHEMYWEISGNDDGVP
                     VVFLHGGPGAGASPMHRRFFDPAHYRIVIFDQRGAGRSRPHGELAGNTTPNLIADMEK
                     LRVRLGIDKWIVFGGSWGSTLALAYGEAHPQRCMALVLRGIFLCRKLEIDWFLYGMRF
                     VYPEAWRKFAEFVPEAERKDLLTAYSKRLNDPDPEVHMPAAHQWSIYEGSCSTLLPSP
                     STVAHFAEDIVALGLARVEAHYFSNSIFLPENSLLENAGRLRSIPGIIVQGRYDMVCP
                     IVSADDLHRAWPEAKYIIVPDAGHSVWEPGISARLVAAMERLKSRGEGRGTGGE"
     gene            complement(4116..5021)
                     /locus_tag="VE030_08025"
     CDS             complement(4116..5021)
                     /locus_tag="VE030_08025"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HZF23398.1"
                     /translation="MHRSIARHLSRLLLLVAALAAGNVLAQKTGATPLIWEVRSAGNA
                     VYLLGSIHLGRSDMYPMGPVVEKAYQASKIVALEADPTDQQAVMAAISGSLYLPPETL
                     QKNLPGPLLARLSRTLERYGIPLEQAQQMKPFLVAITLASIEYAKAGFDPSLGVDMHL
                     ARRAKQDGKRLVELESLGGQIELMNNMSDRLQENLLQVTLESIDNGEIPAVVDSMVKA
                     WRSGDGKKLQDAVSAEERKLPAALAQEFHRKLMSDRNIAMAEKIETILKGSDAHFIAV
                     GAAHLLGQDNVLQILAQKGYRVRPL"
     gene            5211..7490
                     /locus_tag="VE030_08030"
     CDS             5211..7490
                     /locus_tag="VE030_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HZF23399.1"
                     /translation="MDEHLRKSALDYHRLPIPGKISVVPTKGLINQRDLALAYSPGVA
                     AACEEIVNDPGSARNYTARGNLVGVITNGTAVLGLGAIGPLAAKPVMEGKGVLFKKFA
                     GIDVFDIEINERDPDKLVEIIASLEPTFGGLNLEDIKAPECFYIEKQLRSRLKIPVFH
                     DDQHGTAIIVGAAVLNGLKVVGKDIGKVKLVCSGAGAAALACLDLLVSLGLPMKNIWV
                     SDIKGVVYSGRKEEMDDNKVRYAKDTKARTLADIIADADIFLGLSAAKVLKPEMVKKM
                     AQKPLILALANPEPEILPDQAKAVRPDAVIATGRSDYPNQVNNVLCFPFIFRGALDVG
                     ATTINEQMKLAAVHAIAELAQAEQSDIVAIAYGEQDLSFGPEYLIPKPFDPRLIVKIA
                     PAVARAAMESGVATRPIGDFAAYHQQLSQFVYHSGLIMRPVFAVAKKNPSRVVFCEGE
                     DERVLRAVQVVVDEGVARPILIARPEVLNPRIEKFGLRIRPGRDFEMVNPNSDPRFRD
                     YWQEYYALVQRKGVSMEYARREVTRRTTLIGALMVRRGDADAMLCGTIAEHSLHLGYV
                     ANVIGLRKGVNQFAAMNMLILPKRTLFICDTYVNPDPTATQLAEIAMLAADEVQRFGI
                     VPKIALLSHSNFGSSRNPSAQKMSAAREILEQKAPHLEVEGEMHGDAALDEDIRMRLF
                     PNSRLKGEANLLIMPTLDAANISFNLLKITAGEGITVGPMLLGAAKPVHILTPTASVR
                     RLVNMAALASVDALSQRQI"
     gene            7744..8919
                     /locus_tag="VE030_08035"
     CDS             7744..8919
                     /locus_tag="VE030_08035"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZF23400.1"
                     /translation="MTDPGTHPVLFEERRADNGVRAGVARLNAEKSLNSLSLEMIDLL
                     FERLSAWAADPGVAFVVLEAAGEKAFCAGADLHKLYSTMVDHHASAQRDDIRGNAYAL
                     AFFSREYRLDYLIHAYPKPVLCWGHGIVMGGGLGLMSGASHRVVTEHSRVAMPEITIG
                     LYPDVGGTWLLNRAPGRSGLFLALTGAQLNAADTLFARFADYQIAHADKARVMQSLMR
                     QPWTADAADGHRLLTDVLRSVAIALPAPGPLREHLDLIGELCGYATLPEIVAAVTGMK
                     TQDAWLAKAAATLAAGSPGSAALSHALQRRMRHASLAEVFRVELVASTTCAARPDLAE
                     GVRALLIDKDRRPRWRPATLAQVTPEWLEGFFASPWQAHEHPLADLGASSPKASSTR"
     gene            9157..>9297
                     /locus_tag="VE030_08040"
     CDS             9157..>9297
                     /locus_tag="VE030_08040"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZF23401.1"
                     /translation="MDKRLALVTGANRGIGYEICRQLARKGLRVVLTSRDPETGREAR
                     KKL"
BASE COUNT         1940 a         2827 c         2796 g         1734 t
ORIGIN      
        1 cacggaggac acggagaaaa aaacgattcg gaagaagaat gagaatttgg gacggagtat
       61 ttggcgaacg aatgcaatgt gcaccggtgg tgcgaatcgc actagaagca gggacgagga
      121 acgaggggcg aggggccacg aaaggcaaaa ggggaacgat agaagagcaa agagggatac
      181 ataccgaaaa tcgaggcgct gcggcgccgc cccccgccgg gttacctccg gagctgccac
      241 tcccccaaac cgggcgcacc agcataaaat gacggtttgg accggcgtgg ctcccatttt
      301 cctcttagcc agccagggcc caccgaggag actacaaaat gttgcaagca gccgatctga
      361 ccatccgcct caatcccgct gacgacgtgg tgatcgcgcg ggtggagctg cccgccagca
      421 ccgaaatcat caaggaaaaa atccgcactg ccgtgaccat cccggctggt cacaagattg
      481 ccacccacga catcggcgaa ggctcgccgg tgcgccgcta caatcagatc atcggtttcg
      541 cggtcaagcc gatcaaggcc ggagaccacg tacacgtgca caacctggcg atgggtgact
      601 tccagcgcga ctatgccttc tgtgccgact acaagccggt cgattacgtc gccaacccgg
      661 ccactttcca gggcatcgtc cgcgaagacg gtcgtgtggc gacccgcaac tacctcggca
      721 tcatctccag cgtgaactgc tcggcgcacg tctgccgcag catcgccaag tacttcgacg
      781 aagatttcat ggcgcaatac ccgaacgtgg acggcgtggt gccgatccat cacaagaccg
      841 gctgcggcat gggttccgaa ggcgaaccgg tggaactgct gcgccgcgtg ctggcgggct
      901 acgccgtcca ccagaacttc cattcctcgc tgatgatcgg cctgggctgc gaggccaacc
      961 agatcaacaa cctgctggct tcgcagggcc tgaagcgttc gaagcagttg caggccttca
     1021 ccatccagga aaccggcggc accaggaaaa ccgttgccga cggcatcgcc cgcatcaagg
     1081 aactgctgcc cgacgccaac aacgtcaagc gcgagacggt tcccgcgagc catctgactc
     1141 tcgggctgca gtgcggcggc tcggacggct actccgggat ttccgcaaat cccgcgctcg
     1201 gtgctgccgt cgacatcctg gtgcgcaacg gcggcagcgc gattctttcc gaaacgcccg
     1261 agatctacgg cgccgagcac ctgctgacgc gccgcgcgcg ctcgcgcgaa gtcggcgaga
     1321 agctggtgaa gcgcatcaag tggtgggagg actacaccac caggctgggc ggcgagatga
     1381 acaacaatcc ttcgccgggc aacaaggccg gtggcctcac caccatcctg gagaagtcgc
     1441 tgggcgcggt cgccaagggc ggcacgcagg cgctgatgga tgtatacgag tacgccgaac
     1501 gcgttaccgc caaaggcttc gttttcatgg acaccccggg ttacgatccg gtgtcggcga
     1561 ccggccaggt tgctggcggc gccaatatga tctgtttcac caccggccgc ggttcggcct
     1621 acggctgcaa gccgtcgcct tcgctcaagc ttgccaccaa cagcgcgatg ttcaacaagc
     1681 aggaagagga catggacatc aactgcggcg tgatcgtgga cggaaaagcc agcatccagg
     1741 aagtcggcga gcagttcttc cagatgatcc tcaggacagc ttccggcgag cagagcaaaa
     1801 gcgaggcgat gggttacggc gaagacgaat tcgcaccttg ggtgctcggg ccgacgatgt
     1861 aaactcagtg acgagtgacg agtgacaagt gacgagtgac tagcatcgcc actatcttcg
     1921 cctgaagaaa ttgcagtccg ctgcaagccc gaattacaag atcgacaacg acaaaatcgt
     1981 gacgttgagg gcgtttgacc tcgactcgtc acccgtcact tgtcactcgt cactgcggag
     2041 caaagcaatg gctgaagatt catcgaagga gttgttcgag ttcttccaga agatgtggaa
     2101 tccgatgtcg ttccccatcc ccggcatgtt ccagccaacc atgagcgtcg aggaagtgga
     2161 gaaaaaaatc gcggaactgc agtcggtcga gaactggctg aagatgaacc tgaccttcgt
     2221 gcagatgacc atgaagacga tggagatgca gaaatcggca ttgcagtcga tccaggaatc
     2281 tgccgcggcg acctcacaga aggccccaaa gaaacccaaa gacgaggggc gcggaacaaa
     2341 gacttagtgt cctgttccgc taattcgtgg ggactcgcat acccgcgcag tccttcgccc
     2401 tgactgtccc ccgtattgct ctatccactc tccgtttcat gctttttttg catagcagca
     2461 atgccgtccg agctatatac aacccctctc tatggacgat aatggcgcag tgcaatatag
     2521 aagacggggg tcacagtgag tattcttagc catgttttgg aagcattcga taaactattg
     2581 catcaacgtc aaattctcag gcgtgaggcg attctcaccc aggctgaggc cacggaggac
     2641 ttggagtacc ggttgcgccg tctcgacgct aaggccagcc atccggtcct gtacctttaa
     2701 gcggaggccg gcatgatttc agcctttcgt cgcttgttca atacgcgtcg tccgcagatc
     2761 tcggtgcgtt cgcgcgtgaa tcttctgggc acgttcggtt tgctcgagcg taccgacggc
     2821 gtagtcgagt ccataatcgg tgacgaggct tacgtcgagt gggcgaacgg cgcccgctcg
     2881 gtcgaaagca cccggcgcct ggtacggatc agcgcctgat cgagggtcag accgaaacag
     2941 aaaagcccgg cttcgccggg cttttttttg caaattcgat aaaacacgtt taacacgaag
     3001 ggcacgaagg aaattcaagg acgcacgaag gaaggcaaaa aacggatctg atacattcca
     3061 cccctattcc gcctcatcag tggaagtttg gcgcgtgtcc tgcgatagat tctttggttc
     3121 ttactcgccc cccgtccctc gcccctcgcc cctgctctta agtctttcca tcgccgccac
     3181 cagtcgcgcg ctgatgcccg gttcccacac cgagtgaccg gcatcgggca cgatgatgta
     3241 cttcgcttcc ggccaggcgc gatgcaggtc gtctgcggac acgatcggac acaccatgtc
     3301 gtagcgtccc tgaacgatga ttcccggaat agaccgcaac cggcccgcgt tctccagcag
     3361 cgagttctcc ggcagaaaga tgctgttgga aaagtagtgc gcctcgacgc gggctagccc
     3421 cagcgcgacg atgtcctcgg caaagtgcgc aaccgtggaa ggactgggca gcagagtcga
     3481 acaggaacct tcgtagatgc tccactgatg cgctgccggc atatgcactt ccggatcggg
     3541 gtcgttcagc cttttcgagt aagcggtgag caggtccttg cgctccgcct cgggaacgaa
     3601 ctcggcgaac ttgcgccacg cctccggata gacgaagcgc atgccgtaga ggaaccagtc
     3661 gatctccagc ttgcggcaca gaaagatgcc ccgcagtacc agcgccatgc agcgttgcgg
     3721 atgcgcttcg ccataggcca gcgccagcgt gctaccccat gaacctccaa acacaatcca
     3781 cttgtcgatg ccgaggcgca cgcgcagttt ctccatgtcg gcgatcaggt tcggggtggt
     3841 gttgccggcg agttcgccgt gtggacgcga gcggccagcg ccgcgttggt cgaagatcac
     3901 gatgcgatag tgcgccggat cgaagaagcg ccggtgcatc gggctcgcgc ccgcgcccgg
     3961 cccgccgtgc aagaacacca ctggcacgcc gtcgtcgttt cccgatattt cccagtacat
     4021 ctcgtggcgc tggtcgagcc tgagatagcc ggactgatgc ggctctatgt cgggatagag
     4081 ttcggtgcgg attcctgggg cggcgtagtc catcgtcaga gcgggcgcac ccggtaaccc
     4141 ttttgcgcca ggatctgcag aacgttgtcc tgtcccagca aatgcgccgc gccgacggcg
     4201 atgaaatgcg catcggatcc cttgagaatc gtttcgattt tttccgccat cgcgatattg
     4261 cgatccgaca tgagtttccg gtgaaactcc tgcgcgagtg cggccggcag cttgcgctcc
     4321 tctgcggata ccgcgtcctg cagttttttc ccgtcgccgg acctccatgc ttttaccatt
     4381 gaatcgacca ctgccgggat ttccccgttg tctatggatt ccagcgttac ctgcagcaga
     4441 ttttcctgca ggcggtccga catgttgttc atcaattcta tctgacctcc gagcgattcg
     4501 agttccacca gacgcttacc gtcctgcttg gctcgtcggg cgagatgcat gtctacccct
     4561 aaagacgggt cgaatcccgc cttggcgtac tcgatcgaag caagtgtgat cgccaccaga
     4621 aacggcttca tctgttgggc ctgttccagc ggaatcccgt agcgttccag cgtcctggaa
     4681 agtctggcca gcaacggccc cggcaggttc ttctgcagag tttccggcgg cagataaagg
     4741 gagcccgaga tcgccgccat gaccgcctgc tggtcggtag ggtcggcttc gagcgcgacg
     4801 atcttcgatg cctgataggc cttttcgacc accggcccca tcgggtacat gtcgctgcgc
     4861 ccgaggtgaa tcgatcccag cagatacacg gcgttgcccg cagagcgtac ttcccagatc
     4921 agcggggtgg cgcctgtttt ctgcgccagg acattcccgg ctgccagggc cgcgacgagc
     4981 aataacaggc gggagagatg ccgggcgata gaacgatgca tgagacctcg ctgcggaaaa
     5041 tcgggcgacg caccaaccaa catccggccg cattgctgcg gatcggcctt gcagccaacc
     5101 ataacaaaaa ccccgtcctc ccacaaacgc actcatcggg ccgcaagggc cgcagtagtc
     5161 gcgaagtggc tcccgtataa tcgcagcaac ctgttgatta cggagtatcc atggacgagc
     5221 acttgcgcaa gagtgcgctc gactaccacc gcttacccat ccccggcaag atttccgtcg
     5281 ttcccaccaa aggcctgatc aaccagcgcg atctcgcgct tgcctattcg cccggcgtcg
     5341 ccgcagcctg cgaagagatc gtcaacgacc ccggcagcgc gcgcaactac accgcgcgcg
     5401 gcaacctggt cggcgtgatc accaacggaa ctgcggtgct cggactgggc gcaatcggac
     5461 cgctcgcagc caagccggtg atggaaggca agggtgtgct gttcaagaag ttcgccggca
     5521 tcgacgtatt cgacatagaa atcaacgagc gcgatccgga caagctggtg gaaatcatcg
     5581 ccagcctcga gcccacattt ggcggactca atctcgagga catcaaggcg ccggagtgct
     5641 tctacatcga gaagcagctg cgcagccggc tgaagattcc ggtgttccac gacgatcagc
     5701 acggcaccgc catcatcgtc ggcgcagcgg tgctgaacgg cctcaaggta gtcggcaagg
     5761 acatcggcaa ggtgaagctg gtgtgctcgg gggccggagc ggcggcgctg gcctgcctgg
     5821 atctgctggt gagcctcggg ctgccgatga agaacatatg ggtttccgac atcaagggcg
     5881 tggtgtacag cggccgcaag gaggagatgg acgacaataa agtccgctat gccaaagaca
     5941 ccaaggcgcg cacgttagcc gacatcattg cggatgcgga catctttctc ggcctgtcgg
     6001 cagccaaggt gctgaagccg gagatggtga agaaaatggc gcagaagccg ctgatccttg
     6061 cgctcgccaa tcctgagccc gagatcctgc cggaccaggc gaaggcggtg cggccggacg
     6121 ccgtcattgc aaccggccgt tcggattacc cgaatcaggt gaacaacgtg ctgtgctttc
     6181 cattcatctt ccgtggcgcg ctggacgtgg gcgcgaccac catcaacgag cagatgaagc
     6241 tcgcggcggt gcacgcgatc gccgagctgg cgcaggcgga acaatccgac atcgtggcca
     6301 tcgcgtatgg cgagcaggac ctttcgttcg gccccgaata cctgatcccg aagcctttcg
     6361 atccgcggct tatcgtcaag atcgcgccgg cagtggcgag agcggcgatg gaaagcggtg
     6421 tcgccacgcg tccgatcggg gatttcgccg cctaccacca gcaactttcc caattcgtct
     6481 accactccgg tctcatcatg agaccggtat tcgccgtcgc gaagaaaaac cccagccgcg
     6541 tggtgttctg cgagggcgag gacgaacgcg tgctgcgagc cgttcaagtc gtggtggacg
     6601 agggcgtggc caggccgatc ctgatcgccc gcccggaggt gctgaatccg agaatcgaga
     6661 agttcgggct gcgaattcgg cccggccgcg acttcgagat ggtgaacccg aactccgatc
     6721 cgcggtttcg cgactactgg caggagtact acgccctggt gcaacgcaaa ggcgtatcga
     6781 tggaatacgc caggcgtgaa gttacacgcc gcactacgct gatcggcgca ctgatggtac
     6841 gccggggcga cgccgatgcg atgctgtgcg ggaccatcgc cgagcattct ctgcacctcg
     6901 gttacgtcgc caacgtgatc gggttgcgca agggcgtgaa ccagttcgcc gcgatgaaca
     6961 tgctgatcct gccgaaacgc acgctgttca tctgcgatac ctacgtgaac ccggatccga
     7021 ccgcgactca gctggccgag atcgccatgc ttgctgccga cgaagtgcag cgttttggca
     7081 tagtccccaa gatcgcgctg ctgtcgcatt ccaatttcgg cagttcgcgc aatccgtcgg
     7141 cgcagaagat gagcgcggca cgcgagattc tcgaacagaa ggcaccgcat cttgaggtcg
     7201 aaggcgaaat gcacggcgac gcggcgctgg acgaggacat ccgcatgagg ctcttcccga
     7261 actcgcggct caagggcgag gcaaacctgc tgatcatgcc cacgctagac gcggccaata
     7321 tttccttcaa cctgctcaag atcactgccg gcgaaggcat caccgtcgga ccgatgctgc
     7381 tgggcgcggc caagccggtg cacattctca cgcccaccgc cagcgtgcgg cggctggtga
     7441 acatggcggc gctggcctcg gtcgacgcct tatctcaaag gcagatctga aacaccttga
     7501 acacgaaggg tcacgaagga aagacgaagg aacacgaagg agaatcaaaa agcaggaaac
     7561 gcatgaagat aaaaaatgtc ctggaactag ccgcctgtct ggctcataga cacaccgaat
     7621 cgcgccaatg tcatcgacac cgaccgaact agacgtccag ttccgttcgc tttccttcgt
     7681 gtaactttcc ttcgtgatcc ttcgtgttaa ccagcctttt tctgcatcag gattgaacgt
     7741 cgcatgaccg atcccgggac ccatccggta ctattcgaag aacgacgagc ggacaacggc
     7801 gtacgagcgg gcgtcgcgcg cctgaacgcc gagaagtcgc tgaattccct gtcgctggaa
     7861 atgatcgacc tgctcttcga gcgcctatcg gcgtgggcgg ctgatcccgg cgtcgcattc
     7921 gtggtcctgg aggcggccgg cgagaaggcg ttttgcgcag gtgccgacct tcacaaactt
     7981 tacagcacca tggtggatca tcatgcgtcg gcccagcgcg acgatattcg aggcaacgcc
     8041 tacgcactcg cgttcttctc ccgggagtac cggctcgatt acctgattca cgcttatccg
     8101 aagccggtgc tgtgctgggg gcacgggatc gtgatgggag gcggccttgg gctgatgtcg
     8161 ggtgcaagcc atcgcgtggt gaccgagcac tcgcgcgtgg ccatgccgga gatcaccatc
     8221 ggcctgtatc ccgatgtcgg tggtacctgg ctgcttaacc gcgcaccggg acgcagcggc
     8281 ctctttctcg cgctcacggg tgcccagttg aacgctgccg atacgctgtt cgcgcgcttt
     8341 gcggattacc agatcgcaca cgccgacaag gcccgggtca tgcaaagcct gatgcggcaa
     8401 ccgtggactg cggacgccgc ggacggtcat cgcctgctca cggacgtgct gcgcagcgtc
     8461 gcgattgcgc ttccggcgcc cggcccgctg cgcgagcatc tcgacctcat cggcgagttg
     8521 tgcggatacg caacgctgcc ggaaatcgtc gcggccgtca ccggcatgaa aacgcaggat
     8581 gcgtggctgg caaaggctgc tgcgacgctt gccgctggat cgcccggttc ggctgcgttg
     8641 tcgcatgccc tgcagcggcg catgcggcat gcctcgctgg ctgaagtttt ccgcgtcgaa
     8701 ttggtggcgt cgacgacttg cgccgcccgc ccggatcttg ccgagggtgt ccgcgcgctg
     8761 cttatcgaca aggatcgtcg cccgcgctgg cggccggcga cgctggcgca agtgacgcct
     8821 gaatggctcg agggtttctt cgccagtccc tggcaggcgc acgagcatcc gcttgccgac
     8881 ctcggggcgt catcccccaa ggcgtcttca acacgataga tgcttttgcc acagaggaca
     8941 cagagagcac agaggaaaga caggaacaaa gaaatgccgg ggttagaacc tttcatgggc
     9001 tgacgatcca tcacataaca gttgatggtg taggttcgtg aacggtcacc cctcctaatt
     9061 cattttctct ttcctcgttt ttgttcttcc tctgtgttct ctgtgtcctc tgtggctaac
     9121 gagtttgagt cttatcgagg aactgaaatg gcgacaatgg acaaacggct ggcgctggtg
     9181 accggagcca acaggggaat cggctacgag atttgccgtc agcttgcgcg caaggggttg
     9241 cgggtagtgc tgaccagccg cgacccggag acgggccggg aggcccggaa aaagcta
//
LOCUS       DASQOP010000197         6999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_685122, whole genome shotgun sequence.
ACCESSION   DASQOP010000197 DASQOP010000000
VERSION     DASQOP010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6999
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685122"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..1166
                     /locus_tag="VE030_08045"
     CDS             147..1166
                     /locus_tag="VE030_08045"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZF23402.1"
                     /translation="MKKLRILAAFILPGMAAIPAVAQPEKPAVTIAVGGKASLYYLPL
                     SIAERLGYFADEGLKVEILDFAGGAKSLQAMMGGSADVVSGGFDHVLVMRARGQKLKA
                     FVLQIATPAISLGVAKKHAATFRSPKDLKGMKIGVTAPGSSTHAFVNHLLASAGLTSD
                     DVSIIGVGSGPGAAAAMQAGHLDAIANIEPTITLLENSGTIQVMVETVTVQGAKAVFG
                     SPLPAGSLYTREEYVMANPKTVQALTNAMVRALRWLNAASAEDVAKTVPPEYLLGDRG
                     LYLSALRRMRESYSKDGLFPPTGVEAQLKVLIGFEPAVREAKNLNPADSFTNEFVLKA
                     LEKYR"
     gene            1328..1645
                     /locus_tag="VE030_08050"
     CDS             1328..1645
                     /locus_tag="VE030_08050"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZF23403.1"
                     /translation="MLLIIVPMAATAQTDPARAKEIFEQMCAGCHGTYGNGQEGTKSG
                     FVPRIGTLANKEYMASVPDDYLTMIIKKGGAYMGKIAAMPAWEKRLSDDEIRSIVAHI
                     RTF"
     gene            1776..3434
                     /locus_tag="VE030_08055"
     CDS             1776..3434
                     /locus_tag="VE030_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23404.1"
                     /translation="MKILGYAAILALAVGSVSGSAFAQDRAKAEPGGPAGKWSTRTPV
                     KPDPSKIKVPAGYKVGVFASGLDTVTSITVDKNDNVWVAISGNTFGFPPEGIDKPHVK
                     IFDKSGKLIKDNVGLGMFKSFALNEIGYCPENGRTYVGDYSYGVWEIDGVNGTPKLIM
                     NEVPIGDHALGGITCRDGWLYYAVGAPSNSGFSDPNIHGWTDAVDPYWEKRTDAGVPP
                     LPRDPPCRDIVLTGLNIRDTEGNLTGAYLPKGTASKPGQVIKAQKPCGGAIHRAKLKA
                     DSSYTRDDWEVYAMGLRNSSGVAFGPKGSRFEHALVVSDNGMNDKGNRRVANGAEKLF
                     VFTEKGQDAGFPDKEGLNFVNIKRYGPDVYRGNKYDSTRPNPQLYIGDKPFVPTLPPY
                     RFIDHSIGVRGTPLIVANPNPNGYVNPVMEWDTNNPMDGLAWAPKAFGAPGDTIFTAV
                     FGIIDNGPESLRPMWPAIVKIEFLNPTGVKWSIFAENIDPGPNAYQKKENRGGFERTN
                     DVEFSMDGKTMYIADYGELYVNYQMESPFYTTPKSAVVWTITKQ"
     gene            complement(3551..4006)
                     /locus_tag="VE030_08060"
     CDS             complement(3551..4006)
                     /locus_tag="VE030_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23405.1"
                     /translation="MRFVLKASPNLLVTFAALLGFWAVPMTIRAEPPAPHPGHAAPVE
                     AQFRGCESAGWCRFWIEPPDPLAESLYLVRPDGVSRMPGDDAISIAVRDRLNALLASM
                     IHQSKHIVLHDLRALGDGTFAATVTVNEANLASDPILMELREKFTGTIR"
     gene            4176..>6999
                     /gene="uvrA"
                     /locus_tag="VE030_08065"
     CDS             4176..>6999
                     /gene="uvrA"
                     /locus_tag="VE030_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZF23406.1"
                     /translation="MHDVIRIRGARQNNLKKLDLDLPLNELLVVTGVSGSGKSSLVFD
                     TLYAEGQRRYVETFSPYARQFLERMDKPQVDRIEGIPPAIAIDQTNPVRTSRSTVGTM
                     TELTDHLKLLYARAAQLYCRGCGQPVRRDSPDTIFEAMRKRAGEAGDPRLIVTFPLTV
                     PKNFSEAEVKQLLEQQGYTRIHSHRKNFLEVVQDRLRMSSADRSRVIEALEAALRVGQ
                     GKVNIYPLAEDDASRERAGTPHPSPLTPHAPWRFSTALHCAECDIHYADPTPNLFSFN
                     SPLGACETCRGFGRVIGIDFGLIVPDESKTLAGGAIKPWQTPSFHECQDDIEKYAKKR
                     GFPLDKPWRDLSDAHKHWVLEGEPEWVSWKKSWPGTWYGVRRFFDWLETKSYKMHIRV
                     LLSKYRAYTPCSACNGARLKPDALLWRLGTKEDADGVLSAGKRFMPRGLKWSVAQLAA
                     LPGLTVHDLMLLPIDKTRAFFGSLHLPTPLDEATDQLLGEIRARLDYLDRVGLGYLTL
                     DRQSRTLSGGEVQRINLTTALGTSLTNTLFVLDEPSIGLHPRDMGRVIGIMQRLRDSG
                     NSLVVVEHDPQIMLEADRILDIGPGPGERGGEVVFFGTPSELARNGKSLTGDYLSGRK
                     RADAGIAPAPVDSKAARLEVLGAAEHNLKNIDVAIPLNRLVCVTGVSGSGKSTLIQDV
                     LYPALLKHKGKPTELPGAHREIRGHERIAEVVMVDQSPIGRTTRSNPASYVGAWDPIR
                     ELYAKEPAAKERGYTPGTFSFNSGNGRCPTCGGNGFEHVEMQFLSDVYLRCPDCDGKR
                     FRAEVLELTVKGKSIADVLEMTVSEAAVFFAHRPEVIARLQPLVDVGLEYMRLGQPVP
                     TLSGGEAQRLKLAGHLAGARNFSASRLTPNASPEGSLFLFDEPTTGLHFEDVAKLLRA
                     LRKLMAAGHSLVVIEHNLDVIRAADWI"
BASE COUNT         1419 a         2136 c         2199 g         1245 t
ORIGIN      
        1 ggtgcgacac tgcagtttaa tgcgagccag tggtttgtgg cgagtccgct gccgagcagc
       61 ggcaactgca gggtcgacgg ttcccgggta gaatttccgc ccgatcgttc gacccgccta
      121 gcgtttcctc cgtggtagaa aagacgatga aaaaactgcg gatcctcgct gccttcattc
      181 tgcccggtat ggcggcgatc cctgccgttg cgcagccgga gaagcctgcc gtaaccatcg
      241 cggtgggcgg caaggcctcg ctctactacc tgccgctgtc catcgccgag cggctcggct
      301 atttcgcgga tgaaggattg aaggtggaga tcctggattt tgccggcggt gccaagtcgc
      361 tgcaagccat gatgggcggc agtgccgacg tcgtctccgg cggcttcgac cacgtgctgg
      421 tgatgcgcgc gcgcgggcag aaactcaagg catttgtgct gcagattgcc acgccggcga
      481 tttcgctggg tgtggcgaag aagcacgcgg caacgttccg ctcgccgaag gacctcaagg
      541 gcatgaaaat cggcgtgacg gcgccgggat ccagcaccca cgccttcgtc aaccatctgc
      601 tggcatcagc gggcctgact tccgacgacg tctccatcat cggcgtgggc tccggcccgg
      661 gtgcggcggc cgcgatgcag gccggacacc tcgacgcgat cgcgaacatc gaacctacca
      721 tcacgctgct ggaaaactcc ggcacgatcc aggtcatggt ggaaacggtg acggtacagg
      781 gcgccaaggc tgtgttcggc agtcccttgc cggcgggatc gctgtacacg cgcgaggagt
      841 acgtcatggc caatccgaag acggtgcagg cgctgaccaa cgcgatggtg cgggcgctga
      901 gatggctgaa cgctgcttcg gcagaggatg ttgcgaagac cgtgccaccg gagtatctgc
      961 tcggcgaccg ggggctatat ctgtccgcac tgcggagaat gcgcgagagc tactcgaagg
     1021 acggactgtt tccgccgacg ggggttgagg cgcagctcaa ggtgctgatc ggtttcgagc
     1081 cagcagtcag ggaagcaaaa aatctgaatc cggcggattc gtttaccaac gaattcgtgc
     1141 tcaaggcgct ggaaaaatac agataaaggt gaaagtgagt ctcggcccat cctcaatccc
     1201 gcgggccgat cccgtatccc cacttgagcc ctcccgcgac acgtgatacg gtcgcattcc
     1261 ctatccgtca acgacgtttc tttcggtccc gaagacgcga atgaagtatc caataacaat
     1321 cataaccatg ctgctgatca tcgtgccaat ggcggccacg gcacagaccg acccggccag
     1381 agctaaggaa atcttcgagc agatgtgcgc cggatgccac ggcacctacg gtaacggcca
     1441 ggaagggacg aagagcgggt tcgtgccccg catcggcacg ctcgccaaca aggaatacat
     1501 ggcctcggtg cccgatgact atctgacgat gatcatcaag aagggcggcg cctacatggg
     1561 caaaatcgcg gccatgcccg cgtgggagaa gcggctgagc gatgatgaaa tccgcagcat
     1621 cgtcgcgcac atccggacgt tctgactggg accgttctcc gtttcgtcat cgcgttggcc
     1681 agatcggaga cgcattacga aggatctgaa gtggctggtg tgtgggtagc aaggcgggtg
     1741 aagtggattt tcaaccaagc aaggaggagg atgaaatgaa aatattagga tacgcggcaa
     1801 tactcgcatt ggctgtgggg tccgtgtcgg gatccgcttt cgcccaagac cgcgcgaagg
     1861 cggagccggg cggcccggcg ggaaaatggt ctacgcgcac gcccgtcaag ccggatccga
     1921 gcaagatcaa ggtaccggca gggtacaagg tcggagtatt cgcctccggc ctggatacgg
     1981 tcacgtcgat cactgtggac aagaacgaca acgtctgggt cgccatttcg ggaaacacct
     2041 tcggcttccc gccggaaggc atcgacaagc cgcacgtgaa gatcttcgac aagtccggca
     2101 agctgatcaa ggacaacgtc ggtctgggca tgttcaagtc atttgccctg aacgaaatcg
     2161 gctattgccc ggaaaacggc cgtacctacg tcggagacta cagctacgga gtctgggaaa
     2221 tcgacggcgt gaacggcaca cccaagctga taatgaatga ggttccgatc ggtgaccacg
     2281 cgctgggcgg cattacttgt cgggatggct ggttgtatta cgcggtgggc gcgccatcga
     2341 attcgggctt ttccgatccg aacatccatg gctggacgga tgcggtcgat ccctactggg
     2401 aaaagcgcac ggatgccggc gttcccccgt tgccgcgcga cccgccctgc cgggacattg
     2461 tgctgacggg actcaacatc cgcgacaccg aaggcaacct caccggcgcg tacctgccga
     2521 aaggcacggc ttccaagcct ggccaggtga tcaaggcgca aaaaccctgt ggcggcgcca
     2581 ttcaccgggc gaagctgaaa gccgacagca gctatacccg cgacgattgg gaagtgtatg
     2641 cgatgggatt gcgcaactcg tccggtgtcg cgttcggacc caagggctcg cgcttcgagc
     2701 acgcgctggt cgtgagcgac aatggaatga acgacaaggg taatcgccgt gttgccaacg
     2761 gcgccgagaa actgttcgtg ttcaccgaga agggtcagga tgcgggtttt ccggacaagg
     2821 aaggcctgaa cttcgtgaac atcaagcgct acgggcccga cgtttaccgc ggaaacaagt
     2881 acgacagcac acggccgaat cctcagctct acatcgggga caagccattc gtgccgacgc
     2941 ttccgcccta tcgtttcatc gaccattcga tcggggttcg gggcactccg ctgatcgtgg
     3001 cgaatcccaa ccccaatggc tacgtcaacc cggtcatgga gtgggatacc aacaacccta
     3061 tggacgggct ggcttgggcg ccaaaggcat tcggcgcacc tggggacacg atattcaccg
     3121 ccgtgttcgg catcatcgac aacggtccag aaagcctgcg gccgatgtgg ccggcgatcg
     3181 tgaagatcga gttcctcaac ccgaccggcg tcaagtggtc gatctttgcc gagaacatcg
     3241 atcccggtcc caatgcctat cagaagaagg aaaaccgggg cgggttcgag cgaaccaacg
     3301 acgtcgagtt cagcatggat ggcaagacga tgtacattgc ggactacggc gaactctacg
     3361 tgaactacca gatggagtcg ccgttctata cgacgccaaa atccgcggtc gtttggacca
     3421 tcacgaagca gtaggacagg tcagttcttc ccatcccggc ggctgcggcc gccgggaatt
     3481 tttcataaca gggtttccct gcaaatgatt cggccgtcca gggacgccga cagcaaaccc
     3541 gattgcgtta ctaccggatc gtccccgtaa acttctcgcg caattccata agaatcggat
     3601 ctgacgccag atttgcctca ttaacggtca cggtggctgc gaacgttccg tcgcccagtg
     3661 cgcgcagatc atgcagcacg atgtgcttgg attgatggat catgctggcg agcaaggcat
     3721 tgaggcggtc gcgcactgca atggaaatgg catcgtcgcc gggcattcgc gagacgccat
     3781 cgggacgcac gagatacagc gactccgcca ggggatccgg cggttcgatc cagaaccggc
     3841 accagcccgc cgactcacag ccgcgaaact gggcctccac cggtgcggcg tgtccggggt
     3901 ggggtgcggg tggttctgcg cgaatggtca tgggcacggc ccaaaaaccg agcaatgcgg
     3961 cgaaggtaac gagcaagttc ggtgacgctt tgagcacgaa gcgcatgaac ggcgttatac
     4021 cagatatcat tttctggaag caggaacgaa gggaccgatt cctccagcca cggataatgt
     4081 cgcgccggta gaatggcgcc ctttgacggg aacttgcggc ccatgtctgc ctgtcaaagc
     4141 acccggtcac ctgccccgca cccatgccca cacacataca cgacgtcatc cgcatccgcg
     4201 gcgcgcgcca gaataacctc aagaagctcg atctcgatct tccgttgaat gaattgctgg
     4261 tcgtgaccgg cgtgtccggc tccggcaagt cgtcgctggt gttcgacacc ctctatgccg
     4321 aaggccagcg ccgctacgtg gagacattct cgccgtacgc gcgccagttc ctggagcgca
     4381 tggacaagcc tcaggtggac cggatcgagg gcattccacc ggcgatcgcg atcgaccaga
     4441 ccaacccggt gcgcacctcg cgttccacgg ttggcaccat gaccgagctc accgatcact
     4501 tgaaactgct gtacgcgcgt gccgcgcaat tgtactgccg cggttgcggg cagccggtga
     4561 ggcgcgattc gccggatacg atcttcgagg cgatgcgaaa gcgggcaggc gaagccggcg
     4621 atccgcgcct gatcgtcacc tttccgctca ccgtgccgaa gaacttctcc gaggcagagg
     4681 tcaagcagct tctcgagcag cagggctata cacggatcca ctcccaccgt aaaaatttcc
     4741 tggaggtggt gcaggaccgc ctgcgcatgt cgtcggccga ccgctcccgc gtgatcgagg
     4801 cgctggaagc ggcgctcagg gtggggcagg gcaaggtcaa catttatccg ctggccgaag
     4861 acgatgcatc acgcgaacgc gcagggactc ctcacccctc acccctcacg cctcacgcgc
     4921 cgtggagatt ctccacggca ttgcattgcg ccgaatgcga catccattac gccgacccga
     4981 cgccgaacct gttctcgttc aattcgccgc tcggtgcgtg cgaaacctgc cgcggcttcg
     5041 gcagggtgat cggcatcgac ttcgggctga tcgttcccga cgaatcgaag acgctcgcgg
     5101 gcggcgcgat caaaccctgg cagacgccga gctttcacga atgccaggac gacatcgaga
     5161 agtatgcgaa gaagcggggg ttcccgctgg acaagccgtg gcgcgacctg agcgacgcgc
     5221 acaagcattg ggtgctcgaa ggcgaaccgg aatgggtgag ctggaagaaa tcctggcccg
     5281 gcacctggta cggcgtgcgc cgtttcttcg actggctcga aaccaagagc tacaagatgc
     5341 acattcgcgt gctgctctcc aagtatcgcg cctacacccc gtgcagcgcc tgcaacggcg
     5401 cccggcttaa gccggatgcg ctgctgtggc ggctggggac gaaggaagat gccgatgggg
     5461 tactttctgc gggaaagcgc ttcatgccgc gtggcctgaa gtggagcgtt gcgcagctcg
     5521 ccgcgctgcc gggcctgacg gtgcacgacc tgatgctgct gccgatcgac aagacgcggg
     5581 cattcttcgg ttcgctgcat cttcccaccc cgctggatga ggccacggat cagttgctcg
     5641 gggaaatccg cgcgcggctc gactatctcg accgggtcgg gctcggctac ctgacgctgg
     5701 accgccagtc gcgcacgctc tcgggcggcg aagtccagcg catcaatctc acaacggcgc
     5761 tcgggacatc gctcaccaat acgctgttcg tactggacga gccgtcgatc ggcctgcatc
     5821 cgcgcgacat gggcagggtg atcggcatca tgcagcggct ccgcgattcc ggcaattcgc
     5881 tggtggtggt ggagcacgat ccgcagatca tgctcgaagc tgaccgcatt ctcgacatcg
     5941 gccccggtcc gggcgagcgc ggcggtgaag tggtgttctt cggcacgccg tccgagctcg
     6001 cgcgcaacgg caagtcgctc accggcgatt atctttccgg acgcaagcgc gccgacgccg
     6061 gcatcgcgcc ggcgccggtc gattccaagg cggcgaggct cgaggttctg ggcgccgccg
     6121 aacacaatct aaagaacatc gacgtcgcga ttccgctcaa tcgcctggtc tgcgtcaccg
     6181 gcgtcagcgg gtccggtaag tccacgctga tccaggacgt gctttatccg gcgttgttga
     6241 agcacaaggg caagccgacc gagttgcccg gcgcgcaccg ggaaattcgc gggcacgagc
     6301 gcattgccga agtggtgatg gtggaccaga gcccgatcgg acgcaccacg cgctccaatc
     6361 cggcgagcta cgtgggcgcc tgggatccga tccgcgaact ttacgcaaag gagccggcgg
     6421 caaaggagcg cggctatacc cccggcacct tcagcttcaa ctccggcaac ggccgctgcc
     6481 cgacctgcgg cggcaatggc ttcgaacacg tggagatgca gttcctgtcc gacgtgtacc
     6541 tgcgttgccc cgactgcgac ggcaaacgtt ttcgtgccga ggtgctggag ctgaccgtca
     6601 agggcaagtc gatcgcggac gtactggaga tgacggtctc cgaggcggcg gtatttttcg
     6661 cccaccgccc ggaagtcatt gcacggctgc aaccgctggt ggatgtgggc ctggagtaca
     6721 tgaggctcgg ccaaccggtg ccgacgcttt ccgggggcga ggcgcaacgg cttaagctcg
     6781 cgggtcacct cgccggagca agaaattttt ccgcctctcg cctcacgcct aacgcctcgc
     6841 ccgaagggtc cctgttttta ttcgacgagc ccaccaccgg cctgcacttc gaggacgtgg
     6901 ccaagctgct gcgcgccctg cgcaagctga tggcggctgg ccattcgctg gtggtgatcg
     6961 agcacaacct cgacgtgatc cgcgccgccg actggatcg
//
LOCUS       DASQOP010000198         6499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_487494, whole genome shotgun sequence.
ACCESSION   DASQOP010000198 DASQOP010000000
VERSION     DASQOP010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6499
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487494"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            722..2056
                     /locus_tag="VE030_08070"
     CDS             722..2056
                     /locus_tag="VE030_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HZF23407.1"
                     /translation="MNPQNFKRLPLPAKLVTVSWHDLLVIGVPVLLVTALAAWLAVKF
                     IGPAPPDSIVMLAGPKESSYYSIAERYAKIISRSGVKVRVVETEGAQDNLRRLADRKI
                     YADVGFVLGGVTEGIETSGLMSLGSVFVQPVLVFYRGKESVERLSQLEGKRVAIGPEG
                     SGTHALAMKFLVANGMGKGPTRLLELDGDDAIEALKQGKIDAAFVMGELVRGQVTRDL
                     LKVPGIRLMNFSQADGYMRRLKFLSHLTLPKGAFDLGQDLPPHAVDLVGPTVELIARE
                     DLHPALSDLLISAAKEIHGPAGMFRKAGEFPAPLERDFPISEDAQRFYRAGTPFLYKK
                     LPFWMASLVDRVLVVLVPLVVIVIPVIRIVPPLYRWRVRSRIYRWYGALMALERDMFA
                     QPTPERAQEILKRLEDIQEAVNNMRTPLAFADQLYVLREHISGVRNRLAGQH"
     gene            2170..2748
                     /locus_tag="VE030_08075"
     CDS             2170..2748
                     /locus_tag="VE030_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HZF23408.1"
                     /translation="MERLGFNLLAALLLLIGSSGAAWPDDYTDTIKIFREAGASSGFF
                     DNSYGYAIFPTIGKAGIGIGGAYGKGRVYRKGAYVGDTSMKQLTVGWQLGGQGYSQIV
                     FFQDQRAFDEFTSGKFEFGVQATAVAITAGASASANTAGSSASASGGKHDATTAGSYN
                     KGMATFTVAKGGLMFEASIGGQKFTYTPKKRT"
     gene            complement(3091..3318)
                     /locus_tag="VE030_08080"
     CDS             complement(3091..3318)
                     /locus_tag="VE030_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23409.1"
                     /translation="MASRKGAKPMSNSNRQSVASSSAATPSPDLEAIVKWLRGLSAAD
                     FEELSEALTNHNAEKLARFETRIQTIPYRSG"
     gene            complement(3691..4629)
                     /locus_tag="VE030_08085"
     CDS             complement(3691..4629)
                     /locus_tag="VE030_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017330314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HZF23410.1"
                     /translation="MGAVLPFCAAADSPRLQSWPLKTPRRTGIHDVAPAADSGVWFTA
                     QASGHLGYFDPATGRTELIPLGPGSAPHGVIRGPDKAAWITDGGQQAIVRVGWPDRAI
                     RVFPLPEKTPYANLNTCAFDGDGDLWFTGQSGVVGKLAMKTGRTTVWGAPRGRGPYGI
                     CSTPKGDIWWCSLAGSFIATIDRRSGQSTVVEPPTRNQGARRVWSDSRGRIWVSEWLS
                     GNLSMYDPASKNWRTFKVPGDNPQSYAVYVDERDHAWVSEWTGNAVYRFDPATEKFER
                     FGFPRDGANIRQILGRPGEVWLPESGTEFISVIRTV"
     gene            complement(4816..5409)
                     /locus_tag="VE030_08090"
     CDS             complement(4816..5409)
                     /locus_tag="VE030_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23411.1"
                     /translation="MRLLTFLEYAAIVVGAIGILAGRHFALAKGIHLGILLVGVGIAA
                     GGIESLYSRQMSLRFSEEAAQGYDGFPALVWGLMLLLAGGALIGYAYLLDTGAWPRAA
                     GLLKQYPGIVYAAAGLLLAGFSVLLFADPGAHRKRWQTLLLRVPRVILAVVALTCGIA
                     TMASGAWQIADPQGYAQVERQVRARVDAAFKVIIRKS"
     gene            complement(5483..6100)
                     /locus_tag="VE030_08095"
     CDS             complement(5483..6100)
                     /locus_tag="VE030_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23412.1"
                     /translation="MLKTSMQGRIVAVQPRIRLLRSFDQRQHNYPGYALLIERLNEQK
                     QIWIGIGPATHARYGFCAGGSFSAEVEPVANPELEAVDYYKAAGIRYIPGDLQLVSQP
                     PWLGVPPDLPTYRARGHRRLDVRTYENLCYRCIWGCRMAVEMTIDQWNPREKRYRKET
                     FCYGPKSCSKYQAGPVRQVPGRNGTTWQEEDWLDEDATSHRGPDD"
     gene            complement(6209..>6499)
                     /locus_tag="VE030_08100"
     CDS             complement(6209..>6499)
                     /locus_tag="VE030_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23413.1"
                     /translation="KGASLRLLRWKRFVQGDEEQFMANTRRFTELTGVKVRVDSENRR
                     DRLLRIDGDGVVLIDQAATWDLRYGACSPSARLEILASTKAILTSMSGCLRT"
BASE COUNT         1278 a         2008 c         1944 g         1269 t
ORIGIN      
        1 ttgctgctta ttcaaggagt ttgttcattc cgggtttgag agggtagctc taactatcta
       61 ataatgaatg acattctcaa gaaagaccgt cccgtcgccg gctgcatgcg atgcggccgc
      121 gcctacaccg gtagcgtcga aagaaccgtc atgtgtttcg ttcccaacgc cagcgggatg
      181 ccctgcggcg ggagaattgc ctggcgcgcg aacccggagg actggaccga atgtccagcg
      241 tgtagcggca caggcgtgag cgtagaaaaa tcctgtcagc gctgccacgg tgacgggtgg
      301 ctgatgggaa ggcactagcc ttcgaatcaa aaaagcaggc gtcgggccga tttgccctgg
      361 cacagagtca acgaccggcg cggtctgcac ctccaagtcg acggctcacg gaccggcgat
      421 tgcgcaatcg accgtaaagt ccatctccct tttttctgag tcggaaaaaa gctggaacga
      481 attcttgcaa gctacgcgac ctgacttcat ctctctcgca gtcctcgtcg gaaaccctat
      541 acaagtcact cacagcgcga gcgtcattgc gagcgattac ctatggttgg gtgaaggacg
      601 cactcatcac tcatcaccca tcacccttca ccgcactatg ggtaatgcga tcgcggccta
      661 tactgccaat tgcaccgttg atgaagcaaa gaacaggaca ctaaaagaag caggacacta
      721 agtgaacccc caaaatttca aacgcctgcc actgccggcc aaactggtga ccgtgtcctg
      781 gcacgacctg ctcgtgattg gcgtgccggt gctgctggtc accgcgctcg ccgcctggct
      841 ggcggtcaaa ttcatcggcc cggcgccgcc ggattcgatc gtgatgctcg ccgggccgaa
      901 ggaaagcagc tactacagca tcgccgaacg ctacgcgaag atcatctcgc gcagcggcgt
      961 gaaggtgcgg gtggtcgaaa ccgagggcgc acaggacaac ctgcggcggt tggcggatcg
     1021 caagatctac gccgacgtag gattcgtgct gggcggcgtg acggaaggca tcgaaaccag
     1081 cggcctgatg tctctgggca gcgtattcgt gcagccggtc ctggtcttct atcgcggcaa
     1141 ggaatcggtg gaacggctct cccaactgga aggcaagcgc gtcgccatcg gaccggaagg
     1201 cagcggaacc cacgcgctgg cgatgaagtt cctggttgcg aacggcatgg gaaaaggccc
     1261 cacgcgcctg ctggaactgg atggggatga tgcgatcgag gcgctcaagc aggggaagat
     1321 cgacgccgct ttcgtcatgg gcgaactcgt gcgcggccag gtgacgcgcg atctgctgaa
     1381 agtgccgggg atcaggctga tgaacttctc acaggccgac ggctacatgc gccgcctgaa
     1441 gtttctttcc cacctgacgc tgccgaaagg cgcctttgat ctggggcagg accttccacc
     1501 gcacgccgtc gaccttgtag gcccgaccgt tgaactgatc gctcgcgagg acctgcaccc
     1561 cgcgctgtcg gacctgctga tcagcgcagc gaaggagatt cacggccccg cgggcatgtt
     1621 ccggaaggcc ggagaattcc cggcgccgct ggagcgtgat tttccgatca gcgaggatgc
     1681 gcagcgcttc tatcgtgcgg ggacgccctt cctctacaag aaattgccgt tctggatggc
     1741 gagcctggtg gaccgcgtgc tggtggtgct ggtgccgctg gtggtgatcg tgatcccggt
     1801 catacgcatc gtgccgccgc tgtatcgctg gcgggtgcga tcgcgcatct acaggtggta
     1861 cggtgcgctg atggcgctcg agcgtgacat gttcgcccaa ccgacaccgg agcgcgcaca
     1921 ggaaattctc aagcggctcg aagatatcca ggaggcggtg aacaatatgc gcacgccgct
     1981 cgcgttcgcg gaccagctct acgtactgcg cgaacacatc agcggggtgc gtaatcgtct
     2041 ggccgggcag cactgacttc accgttcact cttcaccctt cacccttcac tcttcaccct
     2101 tcacccttca ctcttcaccc ttcactcatt cacgccttca cgccttcacg ccttcacgag
     2161 gtactcgaca tggaacgact tggattcaac cttctcgcag ccctgttgct tttgatcggt
     2221 tcgtcgggtg ccgcgtggcc agacgattac acagatacca tcaagatatt cagggaagcc
     2281 ggggcgagtt cgggcttttt cgacaacagc tacggttacg cgatttttcc gaccatcggc
     2341 aaggccggca taggcatcgg cggcgcctac ggcaagggcc gggtctacag gaaaggcgca
     2401 tacgtcggcg acacctcgat gaaacagctg acggttggtt ggcagctcgg cggccaaggc
     2461 tatagccaga tcgtgttctt tcaggaccag cgcgccttcg acgaattcac ctccggcaag
     2521 ttcgagttcg gcgtccaggc aaccgcggtc gccatcacgg ccggcgcgtc ggcctccgcg
     2581 aacacggccg gcagctccgc cagcgccagc ggaggcaaac atgacgccac gacggccggt
     2641 tcttacaaca agggcatggc aacgttcact gttgccaagg gcgggctgat gttcgaagcg
     2701 agcatcggcg gtcagaaatt cacttatacc cccaagaaac gaacgtaggg ctacacaggc
     2761 acctattgct gtgttattgc tgtgcttgac cctattgctg tgctcggcgt tcctgctgac
     2821 gacgatcccg aaacggaatg tcgacttgca tccggcggcg atccgatctg gggggacgac
     2881 ggtaattgcc gttcggaaat gtcatgtgcc gctcaggctc cccaatggaa ccgcacacgc
     2941 gtacttcaca gggctgatgg gaatcggccc atgccagggt gaacaatatt gcctgggcct
     3001 tagtcggaaa cggaaacggc tgttcgccag ggcttagctc tacttgccaa cccgttgaag
     3061 gcaacggtaa gactttcacc acgcttggga tcatccggag cgataaggaa tggtttgaat
     3121 ccgggtttcg aaacgggcaa gtttttcggc gttgtgattg gttaatgcct cgctcaactc
     3181 ttcaaaatct gcggccgata acccgcgtag ccacttgacg atggcttcga gatccgggct
     3241 gggggtcgcc gcggaactcg acgctactga ttgacggtta gagtttgaca tcggcttcgc
     3301 ccccttgcga gaagccattg tgttttggca ggaagtgccc acgcgaaatt cgtgccatcg
     3361 aactggaacc tttttcgttg gccattgaac gccgattggg gagccaagcg ctcaaaacct
     3421 tgcactgatg gcgatggcct gacaaccctt gacgaaaacc caacagcatc cggtcggcgg
     3481 caagtcgaat gaatggggtt taccatccag atcgttcact cgatgtaatg ggtggcacgg
     3541 agcattcacg tttgaccggg gtgtggccca tggggtccgc cgggctcgtt gaactgacca
     3601 ctcggccccg gaatgccgcc cccatcgtct tgtatcaccc gctgcgtccg ggatggtgcg
     3661 ctgccgcaag atgcccgatg ttgagcggct tcaaaccgtc cggatgaccg agatgaattc
     3721 ggtgccgctc tcggggagcc acacttcgcc aggacggccg aggatctgcc gaatgtttgc
     3781 gccgtcgcga ggaaatccaa agcgctcgaa cttctcggta gcgggatcga agcgataaac
     3841 cgcattgccg gtccactcgc tcacccacgc gtggtcgcgc tcatccacgt agacagcgta
     3901 agactgcggg ttatcgcccg ggaccttgaa agtgcgccag ttcttcgacg cggggtcgta
     3961 catcgagaga ttgccgctca gccattcgct gacccagatg cgcccgcgac tgtcgctcca
     4021 gacacggcgg gcgccttgat tcctggtggg cggttccacc acggtggact gcccgctgcg
     4081 gcggtcgatg gtcgcgatga aggagcccgc cagcgagcac caccatatgt ctcccttagg
     4141 agtgctgcag atgccgtagg gtccgcggcc gcgcggagca ccccacacgg tcgtccttcc
     4201 agttttcatt gcgagcttgc cgaccacgcc gctctgcccc gtgaaccaga gatcaccgtc
     4261 gccatcgaag gcgcaggtgt tgagattggc gtaaggcgtt ttctcgggaa gcgggaatac
     4321 cctgatggcg cgatcgggcc agcccacgcg cacgatcgcc tgctggccgc cgtcggtgat
     4381 ccaggccgcc ttgtcgggac cgcgaatgac gccgtgcggt gccgagccgg gccccagcgg
     4441 gatgagttcg gtgcgtcccg tcgcgggatc gaaatagccg agatgcccgc tcgcctgcgc
     4501 cgtgaaccag acgccgctgt cggcagcagg cgccacgtcg tggatgccgg tgcggcgagg
     4561 cgtcttcagc ggccaggatt gcaggcgcgg ggaatccgcg gctgcgcaga atgggagtac
     4621 tgcgcccatt gccatggcgc ctatcacact caatgcctca cgtcgcgaga tccctttcat
     4681 gctttgctcc ttcaccttca ttcacccttc accctttccc cttcactcat tcacgcctgc
     4741 acgattctta tcgcttgcgt gagggatgcg tcgaagtttg gatctcgcgc agaagggctc
     4801 ttaccattca caccttcacg attttcttat gattaccttg aaggcagcat ccacgcgcgc
     4861 gcgaacctgg cgttcgactt gcgcatatcc ctgcgggtcg gcgatctgcc aggcacccga
     4921 cgccatcgtt gcaatcccgc acgtcagcgc caccaccgca aggatgacgc gcggcacgcg
     4981 cagcagaagc gtctgccacc gcttgcggtg cgcgcccgga tcggcgaaca gcaacaccga
     5041 aaacccggcc agcaacagac ccgcagcggc gtacacgatt ccgggatact gcttcagaag
     5101 gccggccgct cgtggccaag cgccggtatc gagcaggtat gcgtagccga tgagcgcacc
     5161 gcccgcgagc agcagcatca agccccacac gagcgccgga aagccatcgt agccctgcgc
     5221 ggcttcctcg gaaaatcgca ggctcatctg ccgcgaatac agcgactcga ttccgcccgc
     5281 cgcgatcccc acaccgacca gcagaatgcc gagatgaatg cctttcgcca gggcaaagtg
     5341 ccggccggcg aggattccga ttgcgccgac cacgatcgcc gcgtattcga gaaaagtgag
     5401 gagacgcatg caattcagtg tagtcgattc cccgtctccg ctgcgggtga gggcatggag
     5461 tggtcatcag cacgtacgca tcttagtcgt caggcccgcg atgcgaagtt gcatcttcgt
     5521 ccaaccagtc ttcttcttgc caggtcgttc cgtttcggcc cgggacctgg cgaaccgggc
     5581 ctgcctggta cttcgagcag gatttcgggc cgtaacagaa cgtttccttc cggtaccgtt
     5641 tctcccgtgg attccactgg tcgatggtca tttccaccgc catccggcag ccccagatgc
     5701 agcggtagca caaattctcg taagtccgga catcgaggcg acgatgacct cgggcgcggt
     5761 aggtcggcag atcagggggc acgccaagcc acggaggctg cgaaaccaac tgaagatctc
     5821 cgggtatgta ccggattccc gcggccttgt agtagtcgac ggcctccagt tccggattcg
     5881 cgaccggctc gacttccgcc gagaaactcc ctccggcgca gaagccatat cttgcatgag
     5941 tcgcagggcc gatcccgatc cagatctgct tctgctcgtt gagtctttcg atcaacaggg
     6001 catagcctgg ataattgtgc tggcgctggt cgaaggatcg cagcagccgg atgcgcggct
     6061 ggactgcaac gatgcggcct tgcatgcttg tcttcaacat ccagaatgcc ggacgttcga
     6121 aaggccagga acagcgggag tcatggtttc cttcaggcgg gtggcggtaa cttgtcgagc
     6181 gcggtggcca ccgctttgca tgccgcgatc aagtccgtaa gcagccgctc atcgacgtta
     6241 agatcgcctt cgtactcgcc aagatttcta acctggcact tggcgagcac gccccatacc
     6301 gcaggtccca ggtggccgcc tggtcaatga ggacaacacc gtcgccatca attcgaagga
     6361 gacgatcacg gcggttctcg ctgtccaccc gcaccttcac gccggtcagc tcggtgaagc
     6421 ggcgcgtgtt ggccatgaac tgctcttcgt cgccctggac gaagcgcttc cagcgcagca
     6481 atcgcagact cgcgccttt
//
LOCUS       DASQOP010000199         2693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1294410, whole genome shotgun sequence.
ACCESSION   DASQOP010000199 DASQOP010000000
VERSION     DASQOP010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294410"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /gene="tolQ"
                     /locus_tag="VE030_08105"
     CDS             <1..618
                     /gene="tolQ"
                     /locus_tag="VE030_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007584943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HZF23414.1"
                     /translation="FRKMFVIRDAVRRTDQFERSFWSGADLGALYQQAAGSRQNAGSM
                     ERIFEAGFREFVKLRKQTGTNLAVLMDGTRRAMRATYQREMDHLEAHLSFLATVGSVS
                     PYVGLFGTVWGIMNAFRGLANVGQATLAHVAPGIAEALVATAMGLFAAIPAVIAFNHF
                     VRHIERLSTRFESFMEEFSNILQRQANAAAQPPVVGQAQVAATGT"
     gene            625..1041
                     /gene="tolR"
                     /locus_tag="VE030_08110"
     CDS             625..1041
                     /gene="tolR"
                     /locus_tag="VE030_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HZF23415.1"
                     /translation="MRRHPRLMNQINVVPYIDVMLVLLVIFMVTAPLMNPGVIDLPTI
                     GKASAPPAQPIEISIRASGALSYIDRDLSNAERPLRDRDELLNVIKSKQSKKPNQPVV
                     ISADKDVKYEFVIQVMDLLQQNRVKRVGLLAKPRSG"
     gene            1044..2012
                     /gene="tolA"
                     /locus_tag="VE030_08115"
     CDS             1044..2012
                     /gene="tolA"
                     /locus_tag="VE030_08115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HZF23416.1"
                     /translation="MHTVDEPGRVASGILALVVHLLFFGMLVVGISWQKKIVGPVVVD
                     LCEELPEPVTKVSPPPRMVQAPPKPAPEVKKEPPPPPPKVEAPTKPSPAEIELREKVR
                     KQKEEEEAKRLEEKKALEAEKRRLEKEHRAEEEAKRLEQEKLKHEEEARRLEEQKQLE
                     EQRRAEEGRRLEEDAKRLEQEKLKREEERRERAAAVERERQQQEAQRKQREADETKRR
                     AEIAMRAAQQKLIDDWRARIQAKVTARIVMPPDLKGRPEGVYEVTVLPGGDILEIQLK
                     RSSGVPAYDAAVERAINLSSPLPVPDDSDLFQQYFRKFNFTFKPPS"
     gene            2037..>2693
                     /locus_tag="VE030_08120"
     CDS             2037..>2693
                     /locus_tag="VE030_08120"
                     /inference="COORDINATES: protein motif:HMM:NF015980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HZF23417.1"
                     /translation="MFGRLRFFVSLALAFSLHMQEAAHAQLNIDLIGGGANQIPVTIL
                     PFAGEEGFVQRTSQIVAADLQRSGMFRLGSVGSVRPFPTEPSEINYRYWKNEGAEALV
                     IGSVIEKADGHAEVRFRMMDVGKQSQILGFSYTVTVSQLRVTAHKIADLIYEKLTGDV
                     GVFATKICFVTKNADRFELQVADADGYNPEFILAHKEPIISPQWSPDGTRVAYVSFER
                     R"
BASE COUNT          573 a          808 c          863 g          449 t
ORIGIN      
        1 ttccgcaaga tgttcgtgat tcgcgacgcc gtccgccgca ccgaccagtt cgagcgcagc
       61 ttctggagcg gcgccgacct gggcgcgctg tatcagcagg cggcgggctc gcgccaaaac
      121 gccggcagca tggagcgcat tttcgaggcc ggattccgcg aattcgtgaa actgcgcaag
      181 cagaccggga ccaacctggc ggtgctcatg gacggcaccc gccgcgccat gcgcgccacc
      241 taccagcgcg agatggacca tctcgaggcg cacctttcct ttctcgccac ggtgggatcg
      301 gtcagcccct acgtcggcct gttcggcaca gtgtggggaa tcatgaatgc gttccgcggg
      361 ctggcgaacg tcggccaggc taccctggcg cacgtggcgc ccggcatcgc cgaagcgctg
      421 gtcgcgaccg cgatgggtct gtttgccgcg atcccggcag tcatcgcctt caaccacttc
      481 gtacgccaca tcgagcggct ctcgacccgc ttcgagagtt tcatggaaga gttttccaac
      541 atcctgcagc ggcaggccaa cgcggcggcg cagccgccgg tggtgggtca ggcgcaggtc
      601 gcggcaaccg gaacctgacc gcgcatgcgc cgccacccgc gcctgatgaa ccagatcaac
      661 gtcgtgcctt acatcgacgt gatgctggtg ctactggtga tcttcatggt caccgcgccg
      721 ctgatgaatc ccggcgtcat cgatctgccg accatcggca aggcttcggc gccgccggcg
      781 cagccgatcg aaatctcgat ccgcgccagc ggggcgctat cctacatcga tcgcgacctc
      841 tccaacgccg aaaggccgtt gagggaccgg gacgagctgc tgaacgtgat caagagcaaa
      901 cagtccaaga agccaaacca gccggtcgtc atttccgccg acaaggacgt caagtacgag
      961 ttcgtgatcc aggtgatgga cctgctgcaa cagaaccggg tcaaaagggt gggactgctg
     1021 gccaaacccc ggtcgggttg acgatgcaca cggttgacga acccggtcgc gtcgcctccg
     1081 gcatcctcgc gctggtggtg catctgctgt tcttcggcat gctggtagtc ggcatcagtt
     1141 ggcagaagaa aatcgtcgga cccgtggtgg ttgacctatg cgaagaattg cccgagccgg
     1201 tcacgaaagt gagtccgccg ccaaggatgg tgcaggcgcc gcccaagccg gctcccgagg
     1261 tgaagaagga gccgccgcct ccgccaccga aggtggaggc gccgaccaag ccctcgccgg
     1321 ccgagatcga gttacgggaa aaggtccgca agcagaagga ggaggaggaa gccaaacgcc
     1381 tggaggagaa gaaagcgctc gaggcggaga agcgccgact ggaaaaggag caccgtgccg
     1441 aggaagaggc gaagcgcctg gagcaggaaa aactgaagca cgaggaagag gctcgccggc
     1501 tggaagagca gaaacagctt gaagagcagc gccgcgcgga agaagggcgt cggctggagg
     1561 aagatgcgaa gcgactggag caggagaaac tgaaacgcga agaggagcgc cgcgagcgcg
     1621 ccgccgccgt cgagcgcgag cgtcagcaac aggaagcgca gcgcaagcag cgcgaggccg
     1681 atgaaaccaa acgccgcgcc gagatcgcca tgcgcgccgc gcagcagaag ctgatcgatg
     1741 attggagggc gcgcattcag gccaaagtca cagccagaat cgttatgccg ccggacctaa
     1801 aagggcgtcc ggagggtgtc tacgaagtta cagtcctgcc tgggggagat attcttgaaa
     1861 tccagttgaa acgctcgagc ggggtgccgg cctacgatgc tgccgtggaa agggcaatca
     1921 atttgtccag cccgcttccg gtaccggatg attccgacct gtttcagcag tacttcagaa
     1981 aattcaactt tacgttcaag ccaccgtctt aacgtgatat gaaaggatca acgaagatgt
     2041 ttggaaggct gcgcttcttt gtatccctag cgctcgcctt ttccctgcac atgcaggaag
     2101 cggcgcatgc gcaattaaat attgacctga ttggcggcgg cgccaaccag attccggtca
     2161 ccatcctgcc ttttgccggg gaagagggct tcgttcagcg caccagccag atcgtggccg
     2221 ccgatttgca gcgcagcggt atgttccggc tgggctcggt gggcagcgtg cggccctttc
     2281 ccactgagcc ttcggagatc aattaccgct actggaagaa cgagggcgcc gaagcgctgg
     2341 tcatcggcag cgtgatcgag aaagccgacg gacatgcaga ggtgcgtttt cgcatgatgg
     2401 acgtgggcaa gcagtcgcag atcctgggtt tttcctacac ggtgacagtg tcgcagctgc
     2461 gcgtcaccgc ccacaagatt gccgacctga tttacgagaa gctgactggc gacgtcggcg
     2521 tatttgccac caagatctgt ttcgtgacca agaacgccga tcgtttcgaa ctgcaggtgg
     2581 cggatgccga cggctacaac ccggaattca tcctggcgca caaggaaccg atcatctcgc
     2641 cgcaatggtc gcccgacggc actcgcgtcg cctacgtttc cttcgagcgc agg
//
LOCUS       DASQOP010000200         8972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2506701, whole genome shotgun sequence.
ACCESSION   DASQOP010000200 DASQOP010000000
VERSION     DASQOP010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8972
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2506701"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VE030_08125"
     CDS             complement(<1..251)
                     /locus_tag="VE030_08125"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZF23418.1"
                     /translation="MRGSFDLFHCAGAFALALLLLGPARAGDEPKNPFAGDAAAAAEG
                     KHLFLKTGCYSCHGHEAEGAVGPDLTDDEWIYKPTDSMI"
     gene            complement(321..2024)
                     /locus_tag="VE030_08130"
     CDS             complement(321..2024)
                     /locus_tag="VE030_08130"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HZF23419.1"
                     /translation="MTFRIPHGLSRRASGALLALPLALAANQGLAQGVTDDMLLNAHK
                     ESNNWRMVGRDYMSTRFSPLNQVNTRNVKRLVPKWTFSFGVLDAQNTTPLIHNGVMYV
                     TSSHGRTFAVNARTGSMIWQYNHQLPEDVGKNMCCDIGNRGAALYGDKVFVATPDAHV
                     VALDMKTGKPVWDVGLGDYKNAYTMTVAPLVVKGKVIVGLSGAEYPTRLFIEALDANT
                     GKQAWRRYTIPAPGEPGSDTWGSNPDNLKYGGASAWITGSYDPALDTIYWGTGNPNPD
                     WDGVGREGDNLYSNSTLALDPDTGAIKFHFQYTPFDVWDFDGVNEHILADIGGRKIWL
                     HGDRNGFLYSIDRSNGKFVYGKEISKVNWATGFTPEGRPIVNPDKVPSYTYEAKDICP
                     ASEGGKWWNPMSYSPPKQMVFVPSREICTDIKSAKGDRVEGKPHWGIGSIKWNKGHGQ
                     LVAFDAGSGNKKWTVEAPSPFMSGVLTTGGGLVFAGTPEGEFKAYDQESGKELWSYQT
                     GSGIVGSPVSYSIDGKQYVAVPSGFGGWTGLSTIGGGGAPHLKDSRKGGYVTVFGLFD
                     E"
     gene            2347..3501
                     /locus_tag="VE030_08135"
     CDS             2347..3501
                     /locus_tag="VE030_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23420.1"
                     /translation="MAKNVLFFVHGIGVHKIGWINEPDGPVAALNAAMDLYPACFAGK
                     ELSDYLDLVEIRYDDIFDTVLKTWQNLGNSLPANGGFDWTGEVQSLLQSVNGNRETFV
                     RYGGDVLLYCGFDLVARAVRLRVSTIIATKIYQAWLAAGGSPGRVPRFAVVAHSLGTT
                     VAQDALYQLAQAKWDDDLAEVSAKRPALTDNAALSGRDKAAFDSVVAGSRANPDKPIP
                     VGLSALYLVSNTIPLLRQVDGDYALVQSGGTFDCARVYNINHALDPVSRIGGGAANPN
                     PRPGWTNVTVRHIHQRNIHGFGHYLSNPAVHAPIFARLIDPGFTAGCAQSAAALAQAP
                     EWTGFGGTLAGLDQQARQILENELRGLITGESSVAALRAAIEQFIKQLGD"
     gene            3504..4496
                     /locus_tag="VE030_08140"
     CDS             3504..4496
                     /locus_tag="VE030_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23421.1"
                     /translation="MKGMTVPAIVLLLGCAVAHGATLDNDSLAMCDRVFRLTPELRPS
                     ALMEAAAVDLDSGRFDRVYATFASRLDAALTTTNRTSPAATRLLANIRAGTLPPHAFD
                     VVPSDDKQKEIIFESRADQIEIDCAQVAPHEPDMAAVAIMTGWVLGQQLLPALEARAR
                     FVARQSAAHQALLNNGLPMWPWELWLNGKRLGQSDWEPLFKTQWVLMRPTAGIEINTR
                     SRAEGDLEASVAIEPLGLVRYRSDDYSKWWGASLVVTSSTREGIGLGALLRWNNLVFG
                     VTRHQADSPAKSDGTFVFIGVELYQLITKKREDFETWKDLQGRRVNELLNGKTP"
     gene            complement(4527..5783)
                     /locus_tag="VE030_08145"
     CDS             complement(4527..5783)
                     /locus_tag="VE030_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HZF23422.1"
                     /translation="MKLQRLALTLFVAGTAALAATSSFAADSVKIAVIDPLSGPFANV
                     GEAMVRYTQLAADAINARGGVLGGAKIEIIALDSKSSPQEAQLALKAAIDQGVRFINQ
                     SNGSNVAGALVDAVNKHNERNPAQVVLYLNTGAVDPALTNDRCSFWHFRFDADADMKM
                     AALTDAIAQNANTKKVYLLNQDYAFGQAVARAAKEMLPKKRPDIQIVGDELHPLGKIK
                     DFSPYVAKIKASGADTVITGNWGNDMALLVKASREAGFKPDFYTYYAGGLGSPPALGD
                     AGIGHLKQVTQWHANIGNEKVTKHYLAYRDKYKNVKDDYYWTTQYQALEMLAKAMEQT
                     KSADPTKVAKALEGMSFDSDTGEVTMRADNHQLIQPLFVSTYTKVDGKDVKYDVERTG
                     NGFKTDRRIEGKDTVMPTTCKMDRPQ"
     gene            6042..7097
                     /locus_tag="VE030_08150"
     CDS             6042..7097
                     /locus_tag="VE030_08150"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HZF23423.1"
                     /translation="MLSYDVVEWGKPLQKAEKPTPKPAGTEVLLKLKYCGVCHSDVHI
                     REGYFDLGGGKRFNMSDRGMHPPVTLGHEPYGTVIAAGPDAKDAQVGKDRLVCPWIGC
                     GQCARCLEGMDNHCMAPRWIGIQRPGGYADHLLIPHPKYLVDASGIDPMWAATLSCSG
                     LTTYSAVSQLKPIPRDEWVMLLGAGGLGLMAIEMLKALGHEKIISVDIDDAKLAVAKK
                     EGAAATLNSRDPEAARKLQQLAGGPLYGAVDLVGAADTASLALGALRKGGKLIVVGLY
                     GGEIPVSLVLVVQRAWRIQGSSVGSVAELKEVIALARAGKIKPIPIEKRPLSEVSRTL
                     DQLKAGQITGRVVAAIG"
     gene            7312..7758
                     /locus_tag="VE030_08155"
     CDS             7312..7758
                     /locus_tag="VE030_08155"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HZF23424.1"
                     /translation="MKPTASRMSAQDIKEIAAYHNKVRADVGVGPLQWSPALANYAQE
                     WADHLASTNCGMEHRTEGLYGENLFQATAGAYTAVDGAKAWESEKKDYHGGALTRSNW
                     QPSGHYTQMVWRDTRMLGCGEAVCNTMLIVACNYDPPGNVLGRKPF"
     gene            7797..8153
                     /locus_tag="VE030_08160"
     CDS             7797..8153
                     /locus_tag="VE030_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23425.1"
                     /translation="MMGPGKSLTIRPRVIACIVLASAAFLPCMVSAADNTKEEDLRAD
                     IVYRCYNQMGEFGADGVEACVKGELSAMQALKMYPQEASEIVQRCTTRLQPIGWEMVK
                     SCADRDIAAAREAKKD"
     gene            complement(8187..8855)
                     /locus_tag="VE030_08165"
     CDS             complement(8187..8855)
                     /locus_tag="VE030_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23426.1"
                     /translation="MTPKTRKHTARRRDLGLSARAFSILMRLSTPRKIQDYLNAIPIN
                     HEIGGQTILSVREVLRQRRAHCIEGAFFAACALWVHGEPPLVMHLDCDLSDFPHVIAL
                     FRRSGHWGAISKTNGAPLRYRDPIYRSLRELVMSYFHEYYNKRGYKTLRSYSGSFDMR
                     RFEPEHWVTSEKSCWAAHDWLADLRHFPLISSHQARLLSRRDPFERRAAKMVQYPKPA
                     ARKK"
BASE COUNT         1687 a         2632 c         2908 g         1745 t
ORIGIN      
        1 aagatcatag agtcggtcgg cttgtaaatc cattcgtcat cggtgagatc cgggccgacc
       61 gcgccttcgg cttcgtgacc gtgacaggaa tagcatcccg ttttcaggaa caggtgtttg
      121 ccttcggccg cggcggcggc atccccggcg aaaggattct tgggctcgtc cccggcacgc
      181 gcaggaccga gcagcagcaa tgccagcgcg aacgcgccgg cgcagtgaaa caagtcaaaa
      241 gaaccccgca tagtgttttt ctcacgctca aaaaaaggaa gcggcgaacc cgccgccgct
      301 tctcgcatcg acgatcgtat ttattcgtcg aacaggccga acaccgtcac gtaaccaccc
      361 ttgcgactgt ccttgagatg aggtgcgccg ccgccaccga tggtggacaa gccggtccat
      421 ccgccgaagc ccgatggcac cgcgacgtac tgcttgccgt cgatggagta gctgaccggg
      481 ctgccgacga tgccggagcc ggtctggtac gaccacagtt ccttgccgct ttcctggtcg
      541 taggctttga actcaccctc gggcgttccg gcgaatacca ggccgccgcc ggtggtcagc
      601 actccgctca tgaagggcga gggcgcttcc acggtccatt tcttgttgcc gctcccggca
      661 tcgaaagcga ccagttggcc atggcccttg ttccacttga tgctgccgat gccccaatgc
      721 ggcttgcctt cgacgcgatc gcccttcgcg ctcttgatgt ccgtgcatat ttcccggctg
      781 ggaacgaaca ccatctgctt aggggggctg tagctcatcg ggttccacca cttgccgcct
      841 tccgaggccg ggcagatgtc cttcgcctca taggtgtagc tcggcacttt gtcaggattg
      901 acgatgggcc ggccttcggg cgtgaacccg gttgcccagt tcaccttgga gatttccttg
      961 ccgtagacga acttgccgtt gctgcgatcg atgctgtaaa ggaagccgtt gcggtcgccg
     1021 tgcagccaga tcttgcgccc gccgatatcg gcgaggatgt gttcgttcac gccgtcgaaa
     1081 tcccacacgt cgaagggcgt gtactggaag tggaacttga tcgcgccggt gtccgggtcc
     1141 aatgcgaggg tggaattgga gtagaggttg tcgccctcgc gaccgacgcc gtcccagtcg
     1201 ggattcgggt tgccggtgcc ccagtagatg gtgtcgagcg ccgggtcgta tgaaccggtg
     1261 atccaggccg acgctccccc gtacttgaga ttgtcaggat tggaacccca cgtatcggat
     1321 cccggttcgc ccggcgcggg gatcgtgtac cggcgccacg cctgcttacc cgtgttggca
     1381 tcgagcgctt cgatgaacaa ccgcgtcggg tattccgctc ccgagaggcc tacgatcacc
     1441 ttgcccttca cgaccagcgg cgccacggtc atcgtgtagg cgttcttgta gtcgccgagt
     1501 ccaacatccc acaccggctt gccggtcttc atgtcgagcg caacaacgtg ggcatccggg
     1561 gtggccacaa agaccttgtc cccgtagagc gcagcgccac ggttgccgat gtcgcagcac
     1621 atgtttttgc cgacgtcttc cggcagttga tggttgtatt gccagatcat cgagccagtc
     1681 ctggcgttca ccgcgaaggt gcggccgtga gacgaagtga cgtacatcac gccgttgtgt
     1741 atcagcggcg tggtgttctg cgcgtcgagc acgccgaagg aaaacgtcca cttcggaacc
     1801 aggcgcttca cgttcctggt gttgacctgg ttcaagggac tgaagcgcgt gctcatgtaa
     1861 tcgcgtccga ccatgcgcca gttgttggat tccttgtgcg cgttgagcag catgtcgtcg
     1921 gtgacgccct gcgcgagacc ctgattcgcg gccagcgcga gcggaagtgc gagcagagct
     1981 ccggaagcac gacgggaaag accgtgcggt attctgaatg tcatgatttc ttcccctaat
     2041 ctattgttca aactcaaagt aaagaaacta tttcgtcccg ccccgacgtg actcccgatg
     2101 gcgcaagctt gagaaacccg aatacttcgc gttgcttgaa atgacacggc acccgagggt
     2161 cgatcgccgc gagtatccta cactaggcag tgcacgaggg actatccggc gcaggaaaaa
     2221 gtggagtggt gcggctcacg tgactgcgca gtgtgaaccc cgatgatttc ggctggtgaa
     2281 atcgggatac tggaattgca tgagcatcct ggagcggttc gctatcgcac ccaatcggag
     2341 ggggaaatgg ccaagaatgt tctgttcttt gtccacggca tcggcgtgca caagatagga
     2401 tggatcaacg agccggatgg gccggtcgcg gcgctgaacg cggccatgga tttgtatccg
     2461 gcgtgctttg cgggcaagga attgtcggac tacctggacc tggtggagat tcgctacgac
     2521 gacattttcg ataccgtgct caagacctgg cagaacctcg ggaattcgct gcccgcaaac
     2581 ggcgggttcg attggaccgg cgaagtgcag agtcttttgc agtcggtgaa cggcaatcgc
     2641 gaaaccttcg tgcgctatgg cggtgacgtc ctgctctact gcggtttcga tctggtcgcg
     2701 cgcgccgtcc gtcttcgtgt cagtaccatc attgccacga aaatttacca ggcgtggctg
     2761 gccgccgggg gttcgcccgg gcgcgtgccg cgattcgcgg tcgttgcgca ttcattggga
     2821 acgaccgtgg cccaggacgc tctttaccag ctcgcacagg cgaaatggga cgacgatctc
     2881 gccgaggtgt ctgccaagcg gcctgcgctc accgacaatg ctgcgctatc cggcagagac
     2941 aaggccgctt tcgattcggt ggtcgccggt tcccgcgcca atccggacaa gccgattccg
     3001 gtcgggctgt ccgcgcttta tctcgtctcc aataccattc cgcttctgcg gcaagtcgat
     3061 ggagactatg cgctggtgca aagcggaggc acgttcgact gtgcccgggt ctacaacatc
     3121 aatcacgcgc tggatccggt cagccggatc ggtggcggcg cggccaaccc gaacccgcgg
     3181 cccggttgga ccaacgtcac ggtccgccac atccaccaac gcaatatcca tggcttcggc
     3241 cactatctct ccaatcccgc ggtacatgcg cccatttttg cgcggctgat cgaccccgga
     3301 ttcactgccg ggtgcgcaca gtccgcagcg gcgctggcgc aggcgccgga atggacggga
     3361 ttcgggggca cgctcgcggg cctcgaccag caagcaaggc agatcctgga gaacgagttg
     3421 cgaggcctga ttaccggtga gtcgtcggtt gctgccctgc gggccgcgat cgagcagttc
     3481 atcaaacaac tcggagactg atcatgaagg ggatgacggt tccagccatc gttcttttgc
     3541 ttggctgtgc ggttgcgcat ggcgcgacgc tcgacaacga cagtctggcg atgtgcgacc
     3601 gggtatttcg gctcacgccg gaactgcgcc cctccgcgct gatggaggcg gcggccgtgg
     3661 acctcgacag cgggcgcttc gaccgagtgt acgcaacttt tgccagccga ctggatgccg
     3721 cgctcactac aacaaacagg acgtctcccg cggcaacccg gcttctggcc aatatccgtg
     3781 cgggaacgct gccgccgcat gccttcgacg tggtgcccag cgacgacaaa cagaaagaga
     3841 tcatttttga aagccgcgcc gaccagatcg agatcgactg cgcacaagtt gcgccgcacg
     3901 agccggacat ggccgcggtc gcgatcatga ccggctgggt gcttggtcaa caactcctgc
     3961 ccgcactgga ggccagagcg cgattcgtgg cgcggcaatc ggcagcgcat caggctctgc
     4021 tcaacaacgg cctgccgatg tggccgtggg agctgtggct caacggaaag cgcctgggtc
     4081 agagcgactg ggaaccgtta ttcaagaccc agtgggtgct gatgcggccg acggcgggaa
     4141 tcgagatcaa tacgcgcagc cgcgcggaag gcgacctgga ggcgagcgtc gcgatcgaac
     4201 ccctgggcct ggtgcgctac cgcagcgacg actattcaaa atggtggggt gcatcgctgg
     4261 tcgtcacgtc gagtacgcgc gagggcatcg gcctcggggc gcttctgcgc tggaacaacc
     4321 tggtcttcgg ggttacgcgg caccaagccg attcgccggc gaaatccgac ggcactttcg
     4381 tctttatcgg tgtcgagctt tatcagctca tcacgaagaa acgcgaggat ttcgaaacgt
     4441 ggaaggattt acaggggcga agggtgaacg aattgctcaa tggcaagacg ccgtaagccc
     4501 gaggccggcg atggagcagt ccggcactat tgtggtcgat ccatcttgca cgtggtcggc
     4561 atcacggtgt ccttgccttc gatacggcga tcggtcttga atccgttgcc ggtgcgttcg
     4621 acgtcgtact tgacgtcctt gccgtccacc ttggtgtagg tcgaaacgaa cagcggctgg
     4681 atcaattggt gattgtccgc gcgcatcgtc acttcgccgg tgtcgctgtc gaatgacatg
     4741 ccctccagcg ccttggcgac ttttgtcggg tctgcagact tggtttgctc catcgctttc
     4801 gccagcattt ccaacgcctg gtactgggtg gtccagtaat agtcgtcttt cacattcttg
     4861 tacttgtcgc ggtaggccag gtagtgcttc gtgaccttct cgttgccgat gttggcgtgc
     4921 cactgggtga cctgcttgag atggccgatg cccgcgtcgc cgagcgcggg cggggaaccc
     4981 agcccgccgg catagtaggt gtagaaatcg ggtttgaagc cggcttcgcg gcttgctttc
     5041 actagaagcg ccatgtcatt gccccagttg ccggtgatga cggtgtcggc gccggaggcc
     5101 tttattttag cgacgtaagg cgagaaatcc ttgattttgc cgagtggatg caactcatcg
     5161 ccaacgattt ggatgtccgg ccgctttttg gggagcattt ccttcgccgc ccttgcgacc
     5221 gcctgcccga acgcgtaatc ctggttgagc aaatacacct ttttggtatt cgcgttttgc
     5281 gcgatcgcat cagtcagcgc cgccattttc atgtcggcat cggcgtcgaa gcgaaagtgc
     5341 cagaagctgc acctgtcgtt ggtgagcgcc ggatcgacgg caccggtgtt gaggtacaag
     5401 acgacctggg cgggattgcg ctcgttgtgc ttgttgacgg catcgaccag cgcgccggca
     5461 acatttgaac cgttgctctg gttgataaag cgaaccccct ggtcgatggc tgccttgagc
     5521 gcgagttgcg cttcctgcgg actgctcttg ctgtcgagcg caatgatttc tatcttggcg
     5581 ccgccgagca ctccgccgcg tgcattgatg gcatcggcgg caagctgtgt gtagcgcacc
     5641 atggcctcgc cgacgttggc aaacggcccc gacagcggat cgatgaccgc gatcttgacg
     5701 ctgtctgcgg cgaaagatga cgttgcagcc agcgccgcgg tgcccgccac aaacaaggtg
     5761 agcgcaagtc gttgcagttt catggcagtt tcctcctcgt tatgcttctt ttgtgccgcc
     5821 cgctgcctgg ggcggcaggt aacggcaaag acaatattgt gaggccgaaa acaccggaac
     5881 aggaccggcg aaacagccga cgtcagatga taaaccctgg tggctgtaat atgctgctcg
     5941 ctaacgtgta gtcatgcaag gcagctccaa ggcgtttgca tggagcgcgg ccgcccagcc
     6001 ggttcaggcc tgcacgtctg tacattcaga acggaggaga catgctgagt tacgacgtag
     6061 tcgaatgggg caagccgctg caaaaagcgg aaaagccgac gcccaagccg gccgggaccg
     6121 aggtgctgct gaagctgaag tattgcggcg tgtgccacag cgacgtgcac atccgcgagg
     6181 gctatttcga cctcggcggc ggaaagcgtt tcaacatgag cgatcgcggg atgcatccgc
     6241 cggtgacgtt gggccatgag ccctacggca ccgtgatagc ggccgggccg gacgcgaagg
     6301 atgcgcaggt cggcaaggac cgcctggtgt gcccgtggat cggctgcggc cagtgcgcgc
     6361 gctgcctgga gggcatggac aatcactgca tggcgccacg ctggatcggc atccagcgcc
     6421 ccggcggcta tgccgaccat ctgctgattc cgcatccgaa gtacctggtg gacgcgagcg
     6481 gcatagatcc catgtgggcg gcgacgctgt cgtgctccgg gctcacgacg tattccgcag
     6541 tatcgcagct caagccgatt ccccgcgacg agtgggtaat gttgctgggt gcgggcggtc
     6601 tgggactgat ggcgatcgag atgctcaagg ccctcggaca cgagaagatc atttcggtgg
     6661 atatcgacga tgcgaagctg gcggtggcga agaaggaagg tgccgcggcg acgctgaata
     6721 gccgtgaccc ggaggctgcc aggaaactgc agcagctcgc cggcggccct ttgtacggcg
     6781 cggtggatct ggtcggtgcg gcggacactg cgagcctggc gctgggagcg ttgcgcaagg
     6841 gcggaaagct gatcgtggtc ggactttacg gtggggaaat cccggtatcg ctggtgctgg
     6901 tggtgcaacg cgcgtggagg atccagggct ccagcgtcgg cagcgtcgcc gaactgaaag
     6961 aggtgatcgc gctggcgcgc gccggcaaga tcaagccgat cccgatagaa aagcggccgc
     7021 tgtcggaggt cagccgcacg ctcgaccagc tcaaggcggg gcagatcacc ggacgcgtgg
     7081 tcgccgcgat cggatgatcc ggcggaatcg acggcaggcg gggctgatcg tatgaacctg
     7141 cgcggaacgg aagtcacgtt cttgcgggca cgacgagcac gcagaggcct tcgcgtctca
     7201 gtcatcgttt ccgcgctgtc gattctggtg atggccggtt gtgctgctcc cccggcccgg
     7261 ccgcctgccg cgccgacgcc cgctccgccg gcggtaccgg aggcgccggc agtgaaaccc
     7321 accgcatcca ggatgtcggc gcaggacatc aaagagattg ctgcgtatca caacaaggtg
     7381 cgtgccgacg tcggcgtcgg tccgctgcag tggtcgccgg ccctggcgaa ctacgcacag
     7441 gaatgggccg accacctcgc gagcacgaat tgcgggatgg agcatcgcac cgagggtttg
     7501 tacggcgaga atctgttcca ggccaccgcg ggcgcctaca ctgcggtgga cggggccaag
     7561 gcctgggaga gcgaaaagaa ggactatcac ggcggcgccc tgacgcgatc caactggcaa
     7621 ccgtccggac actacacgca gatggtttgg cgtgacaccc ggatgctcgg ctgcggcgag
     7681 gcggtgtgca acacgatgct gatcgtggct tgcaactacg acccgcccgg gaacgtcctc
     7741 gggcgcaagc cattctgata ccggctgcgc gcaaacggag aaatcgggaa acggaaatga
     7801 tgggacccgg gaaatcattg accattcggc cacgggtaat cgcctgcatc gtgttggcaa
     7861 gcgccgcatt cctgccctgt atggtttccg ccgctgataa caccaaagag gaggacctgc
     7921 gggcggacat cgtgtatcgc tgctacaacc agatgggcga attcggggcc gacggggtgg
     7981 aagcctgtgt gaaaggagaa ctttccgcga tgcaggcttt gaagatgtat ccgcaggagg
     8041 ccagtgaaat cgtgcaacgc tgtacgacac ggctgcagcc tataggctgg gaaatggtga
     8101 agtcatgcgc cgacagggac atcgcagccg cgcgggaagc gaaaaaggac tgatagccgg
     8161 gcgaacaccg tttcagccgg cccgattcac ttcttgcgcg cagccggctt gggatactgg
     8221 accatctttg cggcgcggcg ctcgaaggga tcgcggcgcg acagcaggcg cgcctggtgg
     8281 gaggaaatga gcgggaagtg gcgcaggtcc gccagccagt cgtgcgctgc ccagcatgat
     8341 ttttcgctgg tgacccaatg ttcgggttcg aagcgccgca tgtcgaacga tcccgaatag
     8401 ctgcgcagag tcttgtaacc gcgcttgttg tagtactcgt gaaaatacga catgaccagt
     8461 tcgcgcaggc tgcgatagat cgggtcgcgg taacgcagcg gcgcgccgtt ggtcttggag
     8521 atcgcgcccc agtggccgct gcggcggaac aacgcaatga cgtgcgggaa gtcgctcaga
     8581 tcgcagtcca ggtgcatcac cagcggcggc tcgccgtgca cccagagcgc gcaggcggcg
     8641 aaaaaggcgc cttcgatgca atgcgcgcgg cgctgcctga gcacttcgcg caccgacagg
     8701 atggtctgtc cgccgatttc gtgattgatc ggaatcgcgt tcaggtaatc ctggattttc
     8761 cggggcgtgg acaagcgcat caggatcgag aacgcgcgcg cgctgagccc gagatcgcgg
     8821 cggcgtgcag tatgcttgcg ggttttggga gtcatgccac ggttcgtaga gaactaccgg
     8881 agggcggggt ctgagccacg gacggtatcc tgttatggag cgacagtgcg gaaatactac
     8941 ctaagcagtt ggccgacgaa cagcgatacc ag
//
LOCUS       DASQOP010000201        19411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1497335, whole genome shotgun sequence.
ACCESSION   DASQOP010000201 DASQOP010000000
VERSION     DASQOP010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 19411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19411
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497335"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..1267)
                     /locus_tag="VE030_08170"
     CDS             complement(14..1267)
                     /locus_tag="VE030_08170"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HZF23427.1"
                     /translation="MAEIRNYTMNFGPQHPAAHGVLRLVLELDGEVIQRADPHIGLLH
                     RATEKLAEHKTFLQSVPYMDRLDYVSMMCNEHAYVMAIEKLLGIEVPQRAQYIRVMFD
                     EITRVLNHLLWLGAHGLDVGAMTVFLYCFREREDLMDCYEAVSGARLHAAYYRPGGVY
                     RDLPDAMPRYQASKIHDEKAVHELNKNRQGSLLDFIEDFSERFPRYVDEYETLLTDNR
                     IWKQRTVGIGVVAPERALALGFSGPMLRGSGVEWDLRKKRPYAVYDRMDFDIPVGVNG
                     DCYDRYLVRIEEMRQSNHIVRQCIDWLRKNPGPVITDDHKVASPSRVDMKMNMEELIH
                     HFKLFTEGMHVPAGETYAAVEAPKGEFGIYLISDGANKPYRMKIRAPGFAHLAALDEM
                     ARGHMIADVVAIIGTQDIVFGEVDR"
     gene            complement(1742..2341)
                     /locus_tag="VE030_08175"
     CDS             complement(1742..2341)
                     /locus_tag="VE030_08175"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HZF23428.1"
                     /translation="MNTKLAALAENLKSVLGDRIAGIDESHGELTMVVNATDLLWAAA
                     QLRDHAQLRFEQLVDLCGVDYLEFGNRPREGARFAVVYHLLSISRNQRLRLRVFAAED
                     DFPSVDSVNGIWPSANWYERETFDLFGIVFPGHPDLRRILTDYGFIGHPFRKDFPLSG
                     YVEMRYDPDQSRVVYQPVSIEPRETVPRVVREANYGDVE"
     gene            complement(2353..2829)
                     /locus_tag="VE030_08180"
     CDS             complement(2353..2829)
                     /locus_tag="VE030_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HZF23429.1"
                     /translation="MTIEGVTEKGFVTTTIDNVINWTRTGSMWPMTFGLACCAVEMMH
                     AGAARYDLDRFGVVFRPSPRQSDVMIVAGTLCNKMAPALRKVYDQMAEPRWVISMGSC
                     ANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALLYGIIQLQNKIRRTNTIAR"
     gene            complement(2834..3202)
                     /gene="ndhC"
                     /locus_tag="VE030_08185"
     CDS             complement(2834..3202)
                     /gene="ndhC"
                     /locus_tag="VE030_08185"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HZF23430.1"
                     /translation="MLEQYFPILLFIFVGLAVGVALPLLGGGLSRLLGVHRPDPEKLS
                     PYECGFEAFEDARMKFDVRYYLVAILFILFDLEIAFLFPWAVVLGEIGTFGFVSMVVF
                     LAILVIGFVYEWKKGALEWD"
     gene            complement(3307..3391)
                     /locus_tag="VE030_08190"
     tRNA            complement(3307..3391)
                     /locus_tag="VE030_08190"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3355..3357),aa:Leu,seq:gag)
     gene            complement(3481..3846)
                     /gene="secG"
                     /locus_tag="VE030_08195"
     CDS             complement(3481..3846)
                     /gene="secG"
                     /locus_tag="VE030_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HZF23431.1"
                     /translation="MDTLILVLHVAAALCVIGLVLLQHGKGADMGAAFGSGSAGSLFG
                     SSGSANFLSRTTAVLAAVFFLTSLGLTYVASHKGRPEGVMSTHQEAPVEPAKSIPAPT
                     GAMPQAGDGSDSKANEVPK"
     gene            complement(3878..4651)
                     /gene="tpiA"
                     /locus_tag="VE030_08200"
     CDS             complement(3878..4651)
                     /gene="tpiA"
                     /locus_tag="VE030_08200"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HZF23432.1"
                     /translation="MRQRLAVGNWKMHGSLPENQALLDTVAAAVTGLYKATCAVCVPF
                     PYLAQTQSALAGSKLAWGAQNVSQYSKGAFTGEVSAGMLREFGCRYVIVGHSERRQLF
                     GEDDRTVALKAKAVLAAGMVPIICVGETLAERESGVTEDVVGRQVAAVLDELGGEVLK
                     GAVLAYEPVWAIGTGRTATPEQAQAVHTYIRGLLAKRDGNLGDVAPILYGGSVKAANA
                     AELFAIPDVDGGLIGGASLVAQEFIAICKAADAPAGRER"
     gene            complement(4962..5762)
                     /gene="pstB"
                     /locus_tag="VE030_08205"
     CDS             complement(4962..5762)
                     /gene="pstB"
                     /locus_tag="VE030_08205"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HZF23433.1"
                     /translation="MSKITEGTAAKVSLVVKAEALNLSFYYGAFNALKRINMPIYEKR
                     VTALIGPSGCGKSTYLRCFNRMHDLYAGNRYEGEIRLQPDNTNLLNQQVDPIEVRMRI
                     SMVFQKPNPFPKSIYENVAYGLRVRGVRSVATLDEKVEQALRGAALWDEVKDRLNDLA
                     FNLSGGQQQRLCIARALATEPEILLFDEPTSALDPIATASIEELIADLKHKVTILIVT
                     HNMQQAARVSDFTAYMYLGEMIEFDATDTIFIKPKNKATEDYITGRFG"
     gene            complement(5772..6695)
                     /gene="pstA"
                     /locus_tag="VE030_08210"
     CDS             complement(5772..6695)
                     /gene="pstA"
                     /locus_tag="VE030_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HZF23434.1"
                     /translation="MADMTVAEIRALIVKHKRWDLIFATVGVLALMVGVLTFASLFAG
                     MAIDGAHRLSPEFFTNFPSRRPEQAGILSAWVGTTLVMIVTAVTAVPLGVAGAVYLEE
                     YAPKNWMTDIIEINVTNLAGVPSIVYGLLALGLFVYQFGFGQSILSAGLTLALLILPV
                     VIVATREAIRSIPAVIREGAYALGATKWQTTADHILPYSIPGILTGVIIGMARAIGET
                     APVITIGALTFIAFLPPAPFSGDPPAGLFDWLFAPFTVMPIQMFNWTSRPEAAFHQNA
                     AAAGLVLVVMTLSMNALAIYIRYRLRKKIKW"
     gene            complement(6697..7650)
                     /gene="pstC"
                     /locus_tag="VE030_08215"
     CDS             complement(6697..7650)
                     /gene="pstC"
                     /locus_tag="VE030_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HZF23435.1"
                     /translation="MSSVASTHSLNPLADNTVSARLAKRKSRHIRERFIEVILFLAAS
                     VSVAVTVGIVAILVAESYQFFQHVPLRDFLTDTQWTPLFDDAHYGIMVLLSGTLVSSF
                     VALSVAIPLGTIIAIYLSEFAPFSIRELAKPFLELLGGVPTIVYGYFALLYVTPALQY
                     FYPGLPGFNLLSAGLVMGIMIIPYVSSVSEDAMRAVPMSMREGSYAMGATRFQTAIRV
                     VTPAAFSGIAAAYILGISRAVGETMILAVAAGMQPNLTWDPTEPAATITAYIVQVALG
                     DLPHGSIGYQTIFAAGLTLMLLTLLFNILGHMLKRRYRQAY"
     gene            complement(7756..8763)
                     /locus_tag="VE030_08220"
     CDS             complement(7756..8763)
                     /locus_tag="VE030_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HZF23436.1"
                     /translation="MLKTHRFTLLVVAGAFAGALGANAVPAHAQTVKIDGSSTVFPIT
                     EAMAEDFQKAKKGKIRVTVGISGTGGGFKKFCRGETDISDASRPILKKEMDDCRAAGV
                     QYIELPVAYDALTVVIHPDNKWAQTLTVAELKKMWEPAAQGKVKIWKQVNPAWPDQPL
                     KLFGPGADSGTFDYFTEAVNGKAKSSRGDFTASEDDNVLVQGVSRDVNALGYFGFAYY
                     VENKDKLRAVRIADKAGQGVEPSFETVINGTYQPLARPIFIYINSKSLERPEVREFVE
                     FYMNNAEALVREVKYVPLPAKAYAYNVEHANKKVLGTKFGGGNKVGLTIEQLMQLEAK
                     L"
     gene            complement(8875..10239)
                     /gene="glmM"
                     /locus_tag="VE030_08225"
     CDS             complement(8875..10239)
                     /gene="glmM"
                     /locus_tag="VE030_08225"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HZF23437.1"
                     /translation="MTRKYFGTDGVRGRVGELPITPDLVMRLGYAAGKVFAAERDLPS
                     GEHAAVLIGKDTRISGYMLESALQAGLSAAGVDIYLSGPMPTPAVAYLTRALRLQAGI
                     VISASHNPFEDNGIKFFSADGNKLPDAVEHAIEARLDEPLQTMPSAQLGKARRIDDAA
                     GRYIEFCKSTFPNDLDLRGLRLVVDCAHGATYHIAAHVFHELGADVISIGNHPDGLNI
                     NRDYGATHPEALVAAVKAHRAHLGIAFDGDGDRLVMADQAGGLYDGDHSLYVIARFRH
                     GRGTLKGGVVGTLMTNLGLEQALAELGVPFTRAAVGDRYVLEKLIENGWQLGGENSGH
                     IICLDKHTTGDGIVSALQVLHALRAEGRPLADLCGDMRMYPQVLVNVKVRKGFDLQAN
                     GEVRAALKAAETRLKGTGRVLLRASGTEPVIRVMVEGEGRKHITQLADALAETIRAQA
                     HARE"
     gene            complement(10316..11179)
                     /gene="folP"
                     /locus_tag="VE030_08230"
     CDS             complement(10316..11179)
                     /gene="folP"
                     /locus_tag="VE030_08230"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HZF23438.1"
                     /translation="MALPHSSPLTPHAVLKLGRFSLTLERPLIMGVVNVTPDSFSDGG
                     RFFGTQQAVEYARMLVEEGADILDIGGESSRPGAEPVGLDEELRRVMPVLDQLAQLPI
                     PVSVDTCKPEVMRRAIAAGAAMVNDINALREPGALEAVAESQAAVCLMHMQGEPRTMQ
                     QKPHYQDLLTEVTGFLAQRVDTARQAGIARERIVIDPGFGFGKKTGHNLELLRGLRRI
                     AGLGQPVLVGLSRKSLFGKITGKAVADRVYASVAAALLAAERGAALVRVHDVAATRDA
                     LLVWKAVMGYE"
     gene            complement(11294..13186)
                     /gene="ftsH"
                     /locus_tag="VE030_08235"
     CDS             complement(11294..13186)
                     /gene="ftsH"
                     /locus_tag="VE030_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HZF23439.1"
                     /translation="MNNLIKNVAIWLVIALVLMTVFNQFSARQHQTTSMEYSQFIDEV
                     KQGNVAKVTIEGRVLKGVRNDGRKFTTYSPSDPWLVSDLLKAGVIIEAKPEEEPSLLM
                     NIFVSWFPMLLLIGVWIFFMRQMQGGGRGGAFSFGKSRARMLDESTNTVTFADVAGCE
                     EAKEEVSELVDFLRDPGKFQKLGGRIPRGVLMVGSPGTGKTLLARAIAGEAKVPFFSI
                     SGSDFVEMFVGVGAARVRDMFEQAKKHAPCIVFIDEIDAVGRQRGAGLGGGNDEREQT
                     LNQLLVEMDGFETGSGVIVIAATNRPDVLDPALLRPGRFDRQVVVPLPDIRGREQIIM
                     VHMRKVPVAPDVRPDILARGTPGFSGADLANLVNEAALFAARSNKRLVDMEDFERAKD
                     KIMMGAERKSMVMPEHERRNTAYHESGHTVVAKLLPRTDPVHKVTIIPRGRALGVTMQ
                     LPQEDRYSMDREQILQNISVLFGGRIAEEIFMGQMTTGASNDFERATEMARRMVTQWG
                     MSDKLGPMVYGENEGEVFLGRSITTHKNVSETTMQKVDAEIRRIIDEQYNLARKLIED
                     NRDKVEAMARALLEWETLDSDQINDIMEGRPPRPPKPTGAPSTPPSSQGNAPSPAVTA
                     TPAREA"
     gene            complement(13281..13907)
                     /locus_tag="VE030_08240"
     CDS             complement(13281..13907)
                     /locus_tag="VE030_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018025659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HZF23440.1"
                     /translation="MARTKTSKAWMHEHVTDPYVRRAKQEGYRSRAAYKLIEMLEKDK
                     LVRPGMTVVDLGAAPGGWSQVLAPLVGSTGRVIALDVLEMEPVEGVAFIRGDFSETET
                     LERLEKELAGRRIDLVISDMAPNISGVGLADQARSIGLAELALDFACNRLKPGGSFLV
                     KLFQGSGIDEFRKQLAGAFSTAVVRKPKASRGRSSELYLLARGRRPST"
     gene            13939..14292
                     /gene="yhbY"
                     /locus_tag="VE030_08245"
     CDS             13939..14292
                     /gene="yhbY"
                     /locus_tag="VE030_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HZF23441.1"
                     /translation="MANQLTPGERRALKARAHALDPVVLIGDGGLTAAVLKEIDRSLN
                     AHELIKVRVAGEDYDTRSDTLRRVCEDLGATAVQHIGKILIIYRQHPEPDAKSRTRPG
                     RKPRRALKRSFQNRA"
     gene            complement(14326..14802)
                     /gene="greA"
                     /locus_tag="VE030_08250"
     CDS             complement(14326..14802)
                     /gene="greA"
                     /locus_tag="VE030_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003267441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HZF23442.1"
                     /translation="MNKVPLTVKGAELMRAELQQLKTVERPKVIASIQEARTHGDLSE
                     NAEYDAAKERQSFIEGRIVELEGKLANAQIIDPAHVDADGRCVFGATVDLEEVGSGET
                     VTYQIVGDDEADIKSGKISISSPIARALIGKYSGDVAEVKAPGGIKEYEILDVKYL"
     gene            complement(14904..18110)
                     /gene="carB"
                     /locus_tag="VE030_08255"
     CDS             complement(14904..18110)
                     /gene="carB"
                     /locus_tag="VE030_08255"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HZF23443.1"
                     /translation="MPKRTDIKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADVTYIEPITWQMLEKIIAVERPDALLPTMGGQTALNCALDL
                     ARQGVLEKHGVEMIGASREAIDKAEDREKFKKAMTKIGLASPRSSIAHSMEEAMQVQA
                     MVGFPTIIRPSFTLGGTGGGIAYNKEEFVAICERGLDASPTHELLIEESVIGWKEFEM
                     EVVRDRKDNCIIVCSIENLDPMGIHTGDSITVAPAQTLTDKEYQIMRDASIACLREIG
                     VDTGGSNVQFGIDPKDGRMVIIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LRNDITGGATPASFEPTIDYVVTKLPRFAFEKFPQADSRLTTQMKSVGEVMAIGRTFQ
                     ESIQKALRGLETGIDGFDEKTTERETIETELADAGPERILFLADALRIGMSREEAHQL
                     TRIDPWFLAQIEDIIRQEKALAGRLIGDLDRDTLWTLKRAGFSDRRLAKLLNATQDAV
                     RAKRWELNVRPVYKRVDTCAAEFDTRTAYMYSTYEEECEARPTDRRKVMVLGGGPNRI
                     GQGIEFDYCCVHAALALREDGYETIMVNCNPETVSTDYDTSDRLYFEPLTLEDVLEIV
                     AVEKPLGVIVQYGGQTPLKLAQDLDANGVPIIGTSPDMIDCAEDRERFQKMLNRLRLR
                     QPPNRTARNPEAALALAQEIGYPLVVRPSYVLGGRAMEIVHEQRDLERYMREAVKVSN
                     DSPVLLDRFLNDAIEVDVDAICDGGQVLIGGIMEHIEQAGVHSGDSACSLPPYSLSKE
                     LQDELRRQTVAMAKALEVVGLMNVQFAIQNDVVFVLEVNPRASRTVPYVSKATGLALA
                     KIAARCMVGQTLESQGVRGEIVPPYYAVKEAVFPFIKFPGVDTILGPEMKSTGEVMGV
                     ADTFAEAFVKSQLAAGVRLPSGGKVFISVRNSDKPKVVEIARTLAALGCTLLATRGTA
                     AALAEAGLQVAAVNKVAEGRPHVVDMIKNGEIALIINTVEDRRSAIQDSYSIRRSALQ
                     ARVTYYTTVAGARAACTGMQHMRELTAYSLQDLHARLH"
     gene            complement(18275..>19411)
                     /gene="carA"
                     /locus_tag="VE030_08260"
     CDS             complement(18275..>19411)
                     /gene="carA"
                     /locus_tag="VE030_08260"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HZF23444.1"
                     /translation="PALDRQPAVLALADGTIFRGISIGAQTHTAGEVVFNTAITGYQE
                     ILTDPSYCRQIVTLTYPHIGNVGVNPEDIESGKVFAAGLVIRDLPVLASNFRRSQDLS
                     DYLKVQGVAGIAGIDTRKLTRILRVKGAQNGCLMAGQVDEAFAFKQARAFPGLAGMDL
                     AKVVSCSKSYEWNQTEWALGRGYGSQTSPKHHVVAYDYGIKYNILRKLASRGCRLTVL
                     PAQASADQALALKPDGIFLSNGPGDPEPCDYAIKAIRKFLDAGVPTFGICLGHQLLGL
                     ASGAKTVKMKFGHHGANHPVQDLDGGRVMITSQNHGFAVDAKTLPANVRVTHVSLFDG
                     SLQGFERTDKPAFCFQGHPEASPGPHDVDYLFDRFIDLMQKPHR"
BASE COUNT         3582 a         6136 c         5855 g         3838 t
ORIGIN      
        1 atcatcggcg cgttcatctg tccacttccc cgaaaacgat gtcctgggtg ccgatgatgg
       61 cgaccacgtc ggcgatcatg tggccgcgtg ccatttcatc gagcgccgcc agatgggcaa
      121 aacccggtgc gcggattttc atccggtaag gcttgttggc gccgtcggaa atgaggtaga
      181 tgccgaactc gcccttgggc gcctcgaccg cggcgtaggt ttcgcctgcc ggcacgtgca
      241 tgccttcggt gaagagcttg aagtggtgga tcagttcttc catgttcatc ttcatgtcca
      301 cgcgcgaggg cgacgccacc ttgtggtcgt cggtgatgac cgggccggga ttcttgcgca
      361 gccaatcgat gcattgcctg acgatgtggt tcgactggcg catttcctcg atgcgcacca
      421 gataacggtc gtagcagtcg ccgttgacgc cgaccgggat atcgaagtcc atccggtcgt
      481 aaaccgcgta tggacgtttc ttgcgcaggt cccactccac ccccgagccg cgcagcatcg
      541 gtccgctgaa tccgagcgcc agcgcacgtt cgggcgcgac cacgccgata cccaccgtgc
      601 gttgtttcca gatgcggttg tcggtgagca gcgtctcgta ttcgtccaca tatcggggaa
      661 agcgttcgct gaagtcctcg atgaagtcca gcagcgaacc ctgccggttc ttgttgagtt
      721 cgtgcaccgc tttttcgtcg tgaatcttgg aggcctggta acgcggcatg gcatcgggca
      781 ggtcgcgata gaccccgccc gggcgataat aagcagcatg caggcgcgca ccggagaccg
      841 cttcgtagca gtccatcagg tcctcgcgct cgcggaaaca gtacaggaac acggtcatgg
      901 cgccgacgtc gagaccatgc gcccccagcc agagcaggtg gttcagcacc cgggtgattt
      961 cgtcgaacat cacgcggatg tattgcgccc gctgcggaac ctcgatcccg agcagcttct
     1021 cgatcgccat cacataggcg tgctcgttgc acatcatcga cacgtagtcc aggcggtcca
     1081 tgtacggcac cgactggagg aaggtcttgt gctcggcgag tttctcggtc gcgcgatgca
     1141 gcaggccgat gtgcgggtcg gcgcgctgga tcacctcgcc gtccagttcc agcaccagcc
     1201 gcaacacccc gtgcgccgcc gggtgctgcg gcccaaagtt catcgtgtaa ttacgaatct
     1261 cagccatggc aaacccgtat cgacaccagg attcggaatc cgaaggctaa cggtggcaca
     1321 cgatgaactt cagtgcaggc taacttgcct tggcaagtta agtccgcagg acggccggag
     1381 ctaaaattca tggtgtagtt tctgatttcc gccatatctg cgttcgcctg aactacaccg
     1441 taagtaatgc agccacggct acaccatgca tttcagtgca ggctaacttg ccttggcaag
     1501 ttaagtccgc agggcggccg gagctaaagt tcatcgtgta attacgaatc tcagccatcc
     1561 gtattcacca cagagacaca gaggcgcgga ggaaaaccaa ggcaaataaa ctgccaagac
     1621 gccaggaacg cgtaaagaaa tggttggata cgcaccacaa catttgccca atcactcctg
     1681 cagccttctt tgtcggtcat tcggttctct ctgtgcctct gtgtctctgt ggtcagcaga
     1741 tttactcgac gtcgccgtaa tttgcttcac gcaccacccg cggcacggtc tcgcgcggct
     1801 cgatgctgac cggctggtag acgacacggc tctggtccgg gtcgtaacgc atctccacgt
     1861 accctgaaag cgggaagtcc ttgcggaaag gatggcctat gaacccatag tcggtgagga
     1921 tgcggcgcag gtcgggatgg cccggaaaca cgattccgaa cagatcgaaa gtttcgcgct
     1981 cgtaccagtt ggccgacggc cagatcccgt tgaccgagtc caccgagggg aaatcgtcct
     2041 cggcggcgaa gacccgcagc cgcaggcgct gattgcgcga aatcgaaagc aggtggtaga
     2101 caactgcgaa acgcgcgcct tcgcgcgggc gattgccgaa ttcgaggtaa tcgactccgc
     2161 acaagtcgac aagctgctcg aagcggagct gcgcgtggtc gcgcagttgc gcggcggccc
     2221 acaaaagatc cgtcgcattg accaccatcg tcagctcacc atgggactcg tcaatgccgg
     2281 cgatcctgtc gccgagcaca gacttcaggt tttcggcaag tgccgcgagc ttggtgttca
     2341 tggccggagg ggtcagcgcg cgatagtgtt ggtacgccgg atcttgttct ggagctggat
     2401 gatgccgtaa agcagcgctt cggcagtcgg agggcagccg ggaacgtaaa catctaccgg
     2461 aacaatgcgg tcgcagcccc gcaccaccga gtaggaatag tggtaatagc cgccgccgtt
     2521 ggcgcaggag cccatcgaga tgacccagcg cggctcggcc atctggtcgt aaaccttgcg
     2581 cagcgcaggc gccatcttgt tgcacagcgt gccggcgacg atcatcacgt cggattggcg
     2641 cgggctggga cggaatacca cgccgaagcg gtcgaggtcg taccgcgccg cgccggcgtg
     2701 catcatttcg accgcgcagc aggccaggcc gaaggtcatc ggccacatcg agccggtgcg
     2761 cgtccagttg atgacgttgt cgatggtggt ggtgacgaag cctttttcgg ttacgccttc
     2821 gatggtcatg gtctcaatcc cactctaacg cccccttttt ccactcgtag acaaacccga
     2881 tgacaaggat ggcaaggaat acgaccatgg aaacgaaacc aaaggtcccg atttccccga
     2941 gcacgaccgc ccaggggaaa agaaaggcga tttccaggtc gaacaggatg aacaggatgg
     3001 cgacgaggta atagcggacg tcgaacttca tcctggcatc ctcaaaggcc tcgaaaccgc
     3061 actcgtaggg ggaaagcttc tcgggatcgg gacggtgaac gccgagcaat ctgctcaaac
     3121 caccgcccaa caggggaagc gccactccga cggccaggcc gacgaagatg aacagcagaa
     3181 tcggaaagta ctgctcaagc attgaccact ctcgacctgt cagcagacag gatcagaatc
     3241 gattgcgcgc gccaggccgt gccctctgtc tgccgcggag tcatgtctgc cgcgtttttg
     3301 atgctttggt gccgacgggg agactcgaac tcccacgggt taccccacca ccccctcaag
     3361 atggcgtgtc taccaatttc accacgtcgg caatccttga gacgtgaggc atcaggcgtg
     3421 aggcggatcg tgaaagctcg agaatcctgt gcctcactcc tcacccctct cgcctcgccc
     3481 ttacttgggt acttcgtttg ccttcgaatc gctcccgtcg cccgcctgcg gcatcgcgcc
     3541 ggtaggcgcc ggaatgctct tggccggctc caccggcgct tcctggtgcg tggacatcac
     3601 gccctcgggc ctgcccttgt ggctggccac gtaagtcagt ccgaggctgg tgaggaagaa
     3661 taccgccgcc aggactgcgg tcgtgcggct cagaaaattg gccgagccgc tggagccgaa
     3721 caggctgcct gcagagccgc tgccgaatgc cgctcccata tctgcgccct tgccgtgctg
     3781 gaggagcacg agcccgatca cgcacaaggc cgcagccaca tgaagaacaa gaatcaaggt
     3841 atccataagt atcctgtcgt ttcagcgatc agttttttca gcgttcccgc ccggccggcg
     3901 catccgccgc cttgcagatc gcaatgaatt cctgagccac cagtgaagcg ccgccaatca
     3961 atccgccatc cacgtccgga atcgcaaaca attccgcggc gttcgcggcc ttgacgctgc
     4021 ccccgtaaag tatcggggcc acatcgccga ggttgccgtc ccgtttagcc agcaagcccc
     4081 gaatataagt gtgtaccgcc tgcgcctgtt ccggggtcgc agtcctgccg gtaccgatcg
     4141 cccagaccgg ttcgtacgcc aggaccgcgc ctttcagtac ctcgccaccc aattcgtcca
     4201 ggaccgcggc aacctgccgt cccaccacgt cttcggtcac gccgctctcg cgttcagcga
     4261 gcgtttcgcc gacgcaaatg atcgggacca ttcccgccgc cagcaccgct ttcgccttga
     4321 gcgctaccgt gcggtcgtcc tcgccaaaca actgcctgcg ctcggagtgt ccgacgatca
     4381 cgtatcggca gccaaactcg cgcagcatcc cggcagatac ttcgccggta aacgctcctt
     4441 tggaatactg gctgacattc tgggcgcccc atgccagttt cgacccggcc aacgctgact
     4501 gggtctgggc aaggtaagga aaagggacgc agaccgcgca ggtcgcctta tacagcccgg
     4561 tcactgccgc cgcgaccgtg tcgaggaggg cctggttttc tggaaggctg ccgtgcatct
     4621 tccagttgcc caccgccagc cgctgccgca tgcgctatcc caccggaaaa actggtgaat
     4681 tttacctgtc ccggggaagg ggggtcaacg ttgtgcgttg cgaaatggtg gcctgcgcgg
     4741 ccctggggaa cggtgctgag gagaatcagg gcgttagtga cgcaccggcc cgaccattcc
     4801 cgagtgatgg gattggccca agctgctaca gaaaactggc cgcttcgctt acccgaagcg
     4861 tccagttatg tagtggttcc ggccgtcgct ccaaggaacc gagcgactta actttgccgg
     4921 agcaaagtta gccttcgccc ttacagaacc ggaagatcgg atcaaccaaa tcttccggtt
     4981 atgtaatctt cagtggcctt gttcttgggc ttgatgaaga tggtatcggt ggcgtcgaac
     5041 tcgatcatct cgcccagata catgtaagcg gtaaagtccg atacgcgcgc cgcttgctgc
     5101 atattgtggg tgacgatcag gatcgtcacc ttgtgcttga ggtcggcgat cagttcctcg
     5161 atgctcgccg tggcgatcgg gtcgagcgcc gaagtgggtt cgtcgaacag caggatctcc
     5221 ggctcggtgg cgagcgcgcg cgcgatgcaa aggcgctgct gctgcccccc ggagaggttg
     5281 aacgccagat cattgagccg gtccttcact tcgtcccaca acgcggcgcc gcgcagcgcc
     5341 tgctctacct tctcgtcgag tgtcgcgacg ctcctcacgc cgcgcacgcg cagaccgtag
     5401 gccacgttct cgtagatcga tttcgggaac ggattgggct tttgaaacac catgctgatg
     5461 cgcatgcgca cctcaatggg gtccacctgc tggttgagca ggttggtatt gtcgggttgc
     5521 agccggatct cgccttcata gcggttgcct gcgtacaagt cgtgcatgcg gttgaaacag
     5581 cgcaggtacg tcgacttgcc gcacccggag ggtccgatca gcgcggtgac cctcttttcg
     5641 tagatcggca tgttgatgcg tttcaacgca ttgaacgcgc cgtagtagaa actcaggttc
     5701 agcgcctcgg ccttgacgac gaggctcacc ttcgcagcgg ttccttcagt aattttcgac
     5761 atggtgcttc cttaccactt gattttcttt ctgagccggt agcgaatgta aatcgccagg
     5821 gcattcatgg acagcgtcat caccaccagc accaggccgg cagcggcggc gttctggtgg
     5881 aacgccgcct ccggacgcga tgtccagttg aacatctgta tcggcatcac cgtaaacggc
     5941 gcgaacagcc agtcgaacag gccggccgga ggatcgccgc tgaacggcgc cggcggcagg
     6001 aaggcgatga atgtcagcgc gccgatggtg atgaccggcg ccgtctcgcc aatggcacgc
     6061 gccatgccga tgattacccc ggtgagaatt ccaggaatcg aatacggaag gatgtggtcc
     6121 gcagtggtct gccatttggt ggcgcccaat gcgtaagcac cctcgcgtat gactgccggt
     6181 atcgagcgga tcgcctcccg cgtggcgacg atcaccaccg gcaggatcaa cagcgccagg
     6241 gtcaggccgg cggaaagaat gctctgtccg aagccgaact ggtagacgaa caaccccaat
     6301 gcgagcaggc cgtagacgat cgagggcacg cccgccaggt tggtgacgtt gatctcgatg
     6361 atgtctgtca tccagttctt cggcgcgtac tcctcgagat aaaccgcgcc cgccacgccg
     6421 agcggcactg cagtgaccgc cgtgacgatc attaccagcg tagtaccgac ccaggctgaa
     6481 aggatgcccg cctgctcggg ccgccgcgac gggaaattgg tgaaaaattc cggcgacaga
     6541 cggtgcgcac cgtcgattgc catgccggcg aacagggagg caaaagtgag tacgcccacc
     6601 atcagcgcaa ggacgccgac ggtcgcaaag atgaggtccc agcgcttgtg cttgacgatc
     6661 agcgcccgga tctcggctac ggtcatgtcg gccatatcaa taggcctgcc ggtatctgcg
     6721 cttcagcata tgcccaagga tgttgaagag cagcgttaat aacatcaggg tgaggcccgc
     6781 ggcaaaaatg gtctgatagc cgatgctgcc gtgcggcaag tcgccgagcg ccacctgcac
     6841 gatatatgcg gtgatagtgg ctgccggttc cgtcgggtcc caggtcagat tcggctgcat
     6901 gccggcagcg accgcgagga tcatggtttc tcccacggcg cgcgaaatgc ccagaatgta
     6961 agcggcggct attcccgaaa atgccgccgg cgttaccacc cggatcgcgg tctgaaaccg
     7021 cgtggctccc attgcatacg aaccctcgcg catcgacatc ggcaccgcgc gcatcgcgtc
     7081 ttcggacacc gaactcacgt agggaatgat catgatgccc attaccaggc cggccgacaa
     7141 caggttgaag cccggcaggc cggggtagaa atactgcagc gccggggtta cgtacagcag
     7201 cgcaaaataa ccgtagacga tggtcggcac cccccccagc agttcgagga aaggcttcgc
     7261 caattcccga atggaaaacg gcgcgaactc cgacagatag atcgcgatga tggtgcccag
     7321 cgggattgcc accgatagcg cgacaaagga gctgaccagc gtgcctgaaa gcagcaccat
     7381 gatgccgtag tgcgcatcat cgaacagcgg cgtccattgc gtatcggtga ggaaatcccg
     7441 gagcgggacg tgctggaaaa actgatacga ctcggcaacc aggatggcga cgatgccgac
     7501 cgtaaccgca accgataccg atgccgcgag gaaaagaatg acctcgatga agcgctcgcg
     7561 aatatggcgg gatttcctct tcgcgagccg cgcgctgact gtgttatccg ccaagggatt
     7621 caggctgtgg gtggaggcga cggaactcat cggttcggag ccgcggctat tattacagaa
     7681 ttctcagagc acccggaaac cgggcaaaaa acgggagggg cagcgcccct cctgttttga
     7741 ttaaaccgac accgcttaca gcttggcttc gagctgcatc aactgttcta tcgtcagacc
     7801 gactttgttc ccgccgccga acttggtgcc gagtaccttt ttgttggcat gttcgacgtt
     7861 ataagcgtag gcctttgccg gcagcggcac atactttacc tcgcgcacca gcgcttccgc
     7921 gttgttcatg tagaactcga cgaattcccg cacttccggc cgctccagcg acttggaatt
     7981 gatgtaaatg aagatcggcc gtgccagcgg ctggtaagtg ccgttgatta ccgtttcgaa
     8041 cgagggttcg actccctggc cggctttgtc tgcaattctt acggcccgga gcttgtcctt
     8101 gttttcgacg tagtaggcga aaccgaaata gcccagcgcg ttgacgtcgc gggacacccc
     8161 ctgcaccagg acattgtcgt cctcggacgc ggtgaagtcg ccgcggctgg acttggcttt
     8221 gccgttgacc gcttcggtga aatagtcgaa ggtaccggag tcggcgccgg ggccgaacag
     8281 cttcagcggt tgatccggcc atgcgggatt cacctgcttc cagattttca ccttgccctg
     8341 cgcggctggc tcccacatct tcttgagctc ggccaccgtc agcgtctgcg cccacttgtt
     8401 gtcgggatga atcacgacgg tcagcgcatc ataggccacc ggcagctcaa tgtattgcac
     8461 gccggccgcc ctgcagtcgt ccatttcctt tttcaggatc ggacgggacg catcggatat
     8521 gtcggtctcg ccgcggcaga atttcttgaa gccgccgccg gtgccggaaa tgccgaccgt
     8581 taccctgatc ttgcctttct tggccttctg aaagtcctcg gccatggctt cggtgatcgg
     8641 gaatacggtc gaggaaccat cgatcttgac tgtttgggca tgcgccggaa cggcattcgc
     8701 gccgagggca cccgcgaacg cgccagcaac taccaacagc gtgaatcggt gagtcttgag
     8761 catgacgtca atctcctgtc attgtggaat ttcgcgagcc aacattgtat ggctgctctg
     8821 tgacagtttg attgcatggc cctccttgct gcggggccgc gggctcagcg aaactcactc
     8881 gcgagcatga gcctgtgccc ggatggtttc agcaagcgca tcggcgagct gtgtgatgtg
     8941 cttgcgcccc tccccttcca ccatgacccg gatcaccggc tcggttcccg aggcgcgcaa
     9001 gagcacacgg cccgtgcctt ttaggcgggt ttcggcggcc ttgagcgccg cccgcacctc
     9061 accgtttgcc tgcaaatcga agcccttacg caccttcacg ttcaccagca cctgcgggta
     9121 catgcgcatg tccccgcaca gatcagccag cggcctgccc tcggcgcgca atgcgtgcag
     9181 cacttgcagc gcggagacga tgccgtcgcc ggtggtgtgc ttgtcgaggc agatgatgtg
     9241 accggagttc tcgccgccaa gctgccagcc gttctcgatc agcttttcca gcacgtagcg
     9301 gtcacccact gcagcgcgcg tgaagggcac gccaagctcc gcgagcgcct gttccaatcc
     9361 caggttggtc atcagcgtgc cgacaacgcc gcctttgagc gtcccccgcc cgtggcggaa
     9421 gcgcgcgatc acgtaaagcg aatggtcgcc gtcgtatagc ccgcccgcct gatccgccat
     9481 caccagccgg tcgccatcgc catcgaacgc gattccgaga tgcgcccggt gcgccttgac
     9541 cgccgctacc agagcctccg ggtgtgtggc gccgtaatcg cgattgatgt tcagcccgtc
     9601 cgggtggttg ccgatggaga tcacgtccgc gccaagttcg tgaaaaacgt gggccgcgat
     9661 gtgataggtc gcgccgtgcg cgcagtcgac caccagcctc aagccgcgca gatcgagatc
     9721 attaggaaaa gtgctcttgc agaactcgat gtaacggccg gccgcatcgt ctattcgacg
     9781 cgccttgccc agttgcgccg aaggcatggt ttgcagcggc tcatccaggc gcgcttcgat
     9841 cgcatgctcg accgcgtccg gaagcttgtt gccgtcggcg gaaaagaact tgatgccgtt
     9901 gtcttcgaaa gggttgtgcg aggcggaaat aacgataccc gcctgcagcc tcagcgcgcg
     9961 ggtgagatag gctaccgccg gggtcggcat cggcccggag agataaatgt ccacgcccgc
    10021 agccgacagc ccggcctgca aggccgattc gagcatgtaa ccggagatgc gcgtgtcctt
    10081 tccgatcagc accgccgcgt gttcacccga gggcaaatcg cgttccgccg caaacacctt
    10141 gcccgcagca tagccgagac gcatcaccag gtccggcgtg atcggcagct ctcctacgcg
    10201 cccgcgcacg ccgtccgtgc cgaaatactt tctcgtcatg ggtgatgggt gatgggtgac
    10261 gggtgaacgg ccttcgttga catgaatgtt acagcggttt tggcctcatt acccattact
    10321 catagcccat taccgctttc cagaccagca acgcgtcgcg ggtcgccgcc acgtcgtgca
    10381 cgcgaaccag cgcggcgccg cgctcggccg caagaagcgc ggcggccacg ctcgcgtaaa
    10441 cccggtccgc caccgctttg ccggtgattt taccgaatag cgacttgcgg gacagtccca
    10501 ccagcaccgg ttgtccgaga ccggcgatcc tgcgcaggcc gcgcagcagc tcgaggttgt
    10561 gcccggtttt cttgccgaag ccgaagccgg gatcgataac gatgcgttcg cgcgcaatac
    10621 ctgcctgccg cgccgtgtcg acccgctgcg cgagaaaccc cgtcacctcc gtcagcaggt
    10681 cttggtaatg cggtttctgc tgcatggtgc gcggctcacc ctgcatgtgc atcaggcaca
    10741 ccgcggcctg actttccgcc actgcctcga gggcacccgg ctcgcgcaat gcattgatgt
    10801 cgttcaccat cgccgctccg gccgcaatcg cgcggcgcat cacttccggc ttgcaggtgt
    10861 cgaccgatac cggtatcggc agttgcgcga gttgatccag caccggcatc accctgcgca
    10921 attcctcgtc gaggcccacc ggttctgcac ccggccgcga ggactcgccg ccgatgtcga
    10981 gaatgtcggc gccctcctcg accagcattc gggcgtattc cacggcttgc tgcgttccga
    11041 agaagcgtcc gccgtcggaa aacgaatccg gagtgacgtt caccacaccc atgatcagcg
    11101 ggcgttccag cgtgagcgag aatcggccaa gcttcagaac ggcgtgaggg gtgaggggtg
    11161 aggagtgagg aagtgccatc acgttcccgc tggccatggt gtgaagattg tcgaagttca
    11221 agcgcgaatt caagaaacga caagggtgag gattttaccc ctcacccctc tctcctgacc
    11281 cctcacgggg ttgttacgct tcgcgtgccg gcgtggcggt cacagcgggc gacggcgcat
    11341 tgccctgtga ggaagggggc gtgctcggtg cacccgtagg tttcggcgga cgcggcgggc
    11401 ggccttccat gatatcgttg atctggtccg aatcgagcgt ctcccactcc agcagcgccc
    11461 tggccatcgc ctcgaccttg tcgcgattat cctcgatcag cttgcgagca aggttgtact
    11521 gttcgtcgat gatgcggcgg atttccgcgt ccaccttctg catggtggtc tcggacacgt
    11581 tcttgtgggt ggtgatcgaa cggccgagga agacttcgcc ctcgttttcg ccgtacacca
    11641 tcggtccgag cttgtcgctc atgccccact gcgtcaccat acggcgtgcc atctcggtgg
    11701 cgcgttcgaa atcgttcgat gcgccagtgg tcatctgtcc catgaaaatc tcttcggcga
    11761 tgcggccgcc gaacagcacc gagatgttct gcaggatctg ctcgcggtcc atgctgtaac
    11821 ggtcttcctg cggaagctgc atggtcaccc ccaatgcgcg cccgcgcggg atgatggtga
    11881 ctttgtgcac cgggtcggtc ctgggcagca gcttcgccac caccgtgtgt ccggactcgt
    11941 ggtacgcggt gttgcggcgc tcgtgctccg gcatcaccat ggacttgcgc tccgcgccca
    12001 tcattatctt gtccttggcg cgctcgaaat cctccatgtc caccagccgt ttgttcgacc
    12061 gtgccgcgaa cagcgcggct tcgttcacga ggttggccag atcggcgccg gagaaaccgg
    12121 gcgtaccgcg ggcaaggatg tcggggcgca cgtcgggtgc gaccgggacc ttgcgcatgt
    12181 gcaccatgat gatctgctcg cgcccgcgga tgtcgggcag cggcactacc acctggcggt
    12241 cgaaacgccc gggcctgagc agcgccgggt cgagcacatc cggccggttg gtcgcggcga
    12301 tgacgatcac gccggagccg gtctcgaagc cgtccatttc caccagcaac tggttgagcg
    12361 tctgttcgcg ctcgtcgttg ccgccgccga ggccggcgcc gcgctggcgg ccgacagcgt
    12421 cgatttcatc gatgaacacg atgcagggtg cgtgcttctt ggcctgctcg aacatgtcgc
    12481 gcacgcgcgc cgcgccgacg ccgacgaaca tctcgacgaa gtcggatccg gaaatcgaga
    12541 agaacggcac tttggcctcg ccggcgatgg cccgggcaag cagggtcttg ccggtacccg
    12601 gggagccgac catcagcaca ccgcgcggaa tgcggccgcc gagtttctgg aacttaccag
    12661 ggtcgcgcag gaaatccacc aattcggaca cttcttcctt cgcttcctcg cagccggcaa
    12721 catcggcaaa cgtcacggtg ttggtggact cgtcgagcat tcgtgcccgg ctcttgccga
    12781 acgagaacgc gccgccgcgc ccgccgccct gcatctggcg catgaaaaat atccatacgc
    12841 cgatcagcaa cagcatcggg aaccaggaca cgaagatgtt catcagcagc gacggctctt
    12901 cctcgggctt ggcctcgatg atgacgccgg ccttgagcag atcggacacc agccaggggt
    12961 cggagggcga atacgtggtg aacttgcggc cgtcgttcct gacgcctttg agcacgcgac
    13021 cttctatcgt caccttggcg acattgccct gcttcacctc gtcgatgaac tgggaatact
    13081 ccatcgacgt ggtctgatgc tggcgggcgc tgaactggtt gaaaaccgtc atcagcacca
    13141 gcgcaatgac cagccagatc gctacgttct tgatgagatt attcaaaatt gctccttgtg
    13201 ccgcggtgac aaaccgcgaa tttcctggct cattctaaac ccatttgcgc gtgctggccg
    13261 ccgcacgacg ccactgcagt tcacgtggag ggtcgtcttc ccctggccag caggtagagc
    13321 tcgctggagc ggccgcgtga tgccttgggc ttgcgcacca ccgccgtcga aaatgctccg
    13381 gcgagctgct ttctgaactc gtcgatcccc gaaccctgaa ataatttgac cagaaagctg
    13441 ccacccggtt tcagcctgtt gcaggcaaaa tcgagcgcca gttcagccag cccgattgaa
    13501 cgggcctgat ccgccaggcc cacaccgctg atattgggcg ccatgtccga aatcacaagg
    13561 tcgatgcggc ggccggccag ttccttttcc agccgttcca gggtttcggt ctcggagaaa
    13621 tcgccgcgaa tgaaagcaac cccctcgacc ggttccatct ccagcacatc gagcgcaatc
    13681 acgcgtcctg tgctacccac cagcggcgcc agcacctgcg accagccgcc aggcgccgcg
    13741 ccgaggtcca ccaccgtcat tccggggcgc accagcttgt ccttctccag catctcgatc
    13801 agtttatagg ccgcgcgcga gcggtaaccc tcctgctttg cgcgccgcac gtaggggtcg
    13861 gtaacatgct cgtgcatcca ggccttgctg gtcttggttc gtgccattgc cctaaaattt
    13921 ccctttttgg agactctctt ggcaaatcaa ttgacccccg gcgagcggcg agcgctcaag
    13981 gcccgcgccc acgccctcga tcccgtggtt ctgatcggcg acggcgggct caccgcggct
    14041 gtgctcaagg aaatcgaccg cagcctgaac gcgcatgaac tgatcaaggt ccgtgtcgcc
    14101 ggcgaagact atgacacccg gtcggatacg ctgcgccggg tatgcgaaga cctcggtgcg
    14161 actgccgtcc agcatatcgg caagatcctg attatttacc gccagcatcc cgagccggac
    14221 gcaaaatccc ggaccaggcc gggaagaaaa ccgcgtcgtg cgctcaagcg cagttttcag
    14281 aaccgcgctt gatttcccta gtccctagtc cctcatccct agcccctaaa gatacttgac
    14341 gtccaggatc tcgtactcct tgatgccgcc gggtgccttg acttccgcga catcgccgga
    14401 gtatttgccg atcagcgcac gcgcgatcgg cgagctgatg gatatctttc cgctcttgat
    14461 gtcggcttcg tcgtcgccga cgatctggta ggtcacggtc tcgccactgc cgacctcttc
    14521 caggtccacg gtggcaccaa acacgcagcg gccgtcggca tcgacatgcg ccgggtcgat
    14581 gatctgcgca ttggcgagct tgccctccag ttcgacaatg cggccttcga tgaagctctg
    14641 cctttccttc gccgcgtcgt actcggcgtt ttccgacagg tccccgtggg ttcgcgcctc
    14701 ctggatggat gcgatcactt tcggccgctc cacggtcttg agctgttgca actcggcacg
    14761 catcaattcg gcgcctttga cggtcagcgg gactttgttc atctttcaat acctctttgt
    14821 ggctgcgcgg cgtgcgtgct gccgatgatt gttctgccaa tcgcgttcgc gccggatcgg
    14881 cgttcgcggc ccaaccaggc ggctcagtgc agcctggcgt gcaggtcctg caacgaatac
    14941 gcggtgagct cacgcatgtg ctgcatgccg gtacatgcgg cacgcgcgcc cgcgacggtg
    15001 gtgtagtaag tcacgcgcgc ctgcagtgcc gaacgccgaa tcgaatagga atcctggatg
    15061 gcgctgcggc ggtcctccac cgtgttgatg atcagcgcaa tctcgccgtt cttgatcatg
    15121 tctaccacat gtgggcggcc ttccgccacc ttgttcacgg cggcgacctg cagccctgcc
    15181 tcggctagcg cggccgcagt gccgcgggtg gcaagcagcg tgcatcccag tgcggcaagc
    15241 gtgcgcgcga tctcgaccac cttgggttta tcggagttgc gcacgctgat gaacaccttg
    15301 ccgcccgagg gcaggcgcac gccggcggcg agctgcgatt tgacgaaagc ctccgcgaaa
    15361 gtatcggcca cccccatgac ttcgccggtg gacttcatct ccggaccgag gatggtatcc
    15421 acgcccggga acttgataaa ggggaatacc gcctccttga ccgcatagta cggcggaacg
    15481 atttcgcccc tgacgccctg gctttcgagg gtctggccca ccatgcaacg cgccgcaatc
    15541 ttggccagtg ccaggccggt cgccttggag acgtaaggta cggtgcgcga ggcgcgcgga
    15601 ttgacttcca gaacgaatac cacgtcgttc tgaattgcga actgcacgtt catcagtccc
    15661 accacttcca gcgctttcgc catcgccacg gtctgccggc gcagttcgtc ctgcagctct
    15721 ttcgacaggc tgtacggcgg cagcgagcac gccgaatcgc cggagtgcac gccggcctgc
    15781 tcgatgtgct ccatgatgcc gccgatcagg acctgcccgc cgtcgcagat ggcatccacg
    15841 tcgacttcga tggcgtcgtt gaggaagcgg tcgagaagca ccggcgagtc attggaaacc
    15901 ttcaccgcct cgcgcatata gcgctccagg tcgcgctgct cgtgcacgat ttccatcgcc
    15961 cgtccgccca gcacgtacga agggcgcacc accagcgggt agccgatttc ctgcgccagc
    16021 gccagcgccg cctcggggtt gcgcgcggtg cggttgggag gctgcctgag ccgaagccgg
    16081 ttcaacatct tctggaaacg ctcgcggtct tccgcgcagt cgatcatgtc cggactggtg
    16141 ccgatgatgg gcacaccgtt cgcatccagg tcctgggcga gtttcagcgg cgtctgcccg
    16201 ccgtattgca cgatcacgcc gagcggcttc tccaccgcga cgatctcgag cacgtcttcc
    16261 agagtcagcg gctcgaagta cagccggtcg gaggtgtcgt aatcggtgga caccgtttcc
    16321 gggttgcagt tgaccatgat ggtctcgtag ccgtcttcgc gcagcgccag cgccgcatgc
    16381 acgcagcagt agtcgaactc gatgccctgg ccgatccggt tcggcccgcc gcccagcacc
    16441 atcaccttcc tgcggtcggt cgggcgcgcc tcgcattcct cctcgtaggt ggagtacatg
    16501 taagcggtgc gggtgtcgaa ctcggcggcg caggtgtcca cccgcttgta caccggccgt
    16561 acgttgagct cccagcgctt ggcgcgcacc gcgtcctggg tggcgttcag cagcttcgcc
    16621 agcctgcggt cggagaagcc ggcgcgcttg agagtccaca gcgtgtcgcg atcgaggtcg
    16681 ccgataagcc tgcctgccag cgccttctcc tggcggatga tgtcctcgat ctgcgccagg
    16741 aaccagggat cgatccgggt cagttggtgc gcctcctccc ggctcatgcc gatgcgcagc
    16801 gcgtcggcga ggaacaggat gcgctccggg ccggcatcgg cgagttcggt ctcgatggtc
    16861 tcgcgctcag tggtcttttc gtcgaatccg tcgatgccgg tttccagccc gcgcagggct
    16921 ttctgtatcg actcctggaa ggtgcggccg atcgccatta cctcgcccac cgacttcatc
    16981 tgggtggtga gcctcgaatc ggcctgcggg aatttctcga aagcgaagcg cggaagcttg
    17041 gttaccacgt agtcgatggt gggctcgaac gaagccggcg tcgccccgcc ggtaatgtcg
    17101 ttgcgcagct cgtccagcgt gtaaccaact gcgagcttgg ccgcgacctt ggcgatcggg
    17161 aagccggtcg cttttgatgc cagcgcggac gaacgcgata cccgcggatt catctcgatg
    17221 atgaccatgc ggccgtcctt cgggtcgatg ccgaactgca cgttggaacc gccggtatcg
    17281 accccgatct cgcgcaggca cgcgatcgag gcatcgcgca tgatctggta ttccttgtcg
    17341 gtgagcgtct gcgccggcgc cacggtgatc gaatccccgg tatggatgcc catcgggtcc
    17401 aggttctcga tcgagcagac gatgatgcaa ttgtccttgc ggtcgcgcac cacctccatc
    17461 tcgaactctt tccagccgat caccgactcc tcgatgagca actcgtgggt gggcgaggcg
    17521 tccagcccgc gttcgcagat ggcgacgaac tcttccttgt tgtaagcaat gccgccgccg
    17581 gtgccgccga gcgtgaacga aggccggatg atggtgggaa agccgaccat cgcctgcacc
    17641 tgcatcgcct cctccatgct gtgggcgatg gacgagcgcg ggctcgcgag gccgatcttc
    17701 gtcatcgcct tcttgaattt ctcgcggtcc tcggccttgt cgatcgcctc cctggaggca
    17761 ccgatcattt ccacgccgtg cttttccagc acgccctgcc tggcgaggtc gagcgcgcag
    17821 ttgagcgcgg tctggccgcc catggtggga agcaacgcat ctgggcgttc gacggcgatt
    17881 attttctcca gcatctgcca ggtgatcggc tcgatgtagg taacgtccgc catttccggg
    17941 tcggtcatga tggtcgccgg attggaattc accaggatga cgcgataacc ctcctcgcgc
    18001 agcgccttgc aggcctgggc gccggagtag tcgaactcgc acgcctggcc gatgacgatg
    18061 gggccggcgc cgatgatcag aatgcttttt atgtcggtgc gctttggcat tgtgaattaa
    18121 ccacagagac acagaggcac ggagaaaatc gtaaagatcg aaaaacagga cacctgcaat
    18181 tgcgggcgct tcccgatctt gatttcctca ttacgcaagc ctctaatctg tctctccagg
    18241 tttcctctgt gtctcggtgt ctctgtggtg aaatctacct gtgcggcttt tgcatcaggt
    18301 caatgaagcg gtcgaacagg tagtcgacgt cgtgcggacc tgggctcgct tcgggatggc
    18361 cctggaagca gaatgccggc ttgtcggtgc gctcgaatcc ctgcaggctg ccgtcgaaca
    18421 acgatacgtg cgtgacccgg acatttgccg gcagcgtctt ggcgtccacc gcgaagccgt
    18481 ggttctggct ggtgatcatc acccgcccgc cgtccaggtc ctgcaccgga tggttggcgc
    18541 cgtggtggcc gaatttcatc ttgaccgtct ttgcgccgct ggccagtccc agcaactggt
    18601 ggccgaggca gatgccgaac gtgggaacgc cggcgtcgag gaacttacgg atggccttga
    18661 tcgcgtaatc gcagggctcg ggatcgccag gaccgttgga aagaaaaatc ccgtctggct
    18721 tgagcgccag tgcctgatcc gcggatgcct gcgccggcag gacggtcagg cggcagccgc
    18781 ggctggcgag cttgcgcagg atgttgtact tgatgccgta gtcgtacgcc accacgtgat
    18841 gcttcgggga agtctggctg ccgtatcccc gtcccagcgc ccattcggtc tggttccatt
    18901 cgtacgattt gctgcagctc accaccttcg ccaggtccat gccggccaac ccgggaaagg
    18961 cccgcgcctg cttgaaggca aacgcctcgt ccacctggcc tgccataagg cagccgtttt
    19021 gcgcaccctt tactcgcagt atccgggtga gcttgcgggt atcgatgccg gcgatccccg
    19081 caacgccctg caccttcagg tagtcggaca ggtcctggct gcggcggaag ttgctggcaa
    19141 gcaccggcag gtcgcggatc accaatcccg cggcaaagac cttgccggat tcaatgtctt
    19201 ccggattgac gccgacgttg ccgatgtgtg gatacgtcag ggtgacgatc tgccggcaat
    19261 acgaggggtc ggtaagaatt tcctggtagc cggtgatcgc ggtgttgaac acgacttcgc
    19321 cggcggtgtg ggtctgcgca ccgatggaaa tgccccggaa tatcgtgccg tcggctaacg
    19381 cgaggacagc aggctgacgg tcgagcgcag g
//
LOCUS       DASQOP010000202        10830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2303974, whole genome shotgun sequence.
ACCESSION   DASQOP010000202 DASQOP010000000
VERSION     DASQOP010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10830
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2303974"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VE030_08265"
     CDS             complement(<1..244)
                     /locus_tag="VE030_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HZF23445.1"
                     /translation="MPNFNANLSMMFNEVSFLERFGAAAKAGFRGVEFLFPYEFPAAQ
                     IKEQLDKHNLQMILFNMPPGDFAAGDRGMACEPAKVG"
     gene            365..1207
                     /locus_tag="VE030_08270"
     CDS             365..1207
                     /locus_tag="VE030_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZF23446.1"
                     /translation="MKFKGTETYVATEDLMMAVNAAVTLERPLLIKGEPGTGKTMLAI
                     EVAKALERPLYQWHVKSTSKAQQGLYEYDAVSRLRDSQLGDARVHDIANYIVRGPLWD
                     AFESDRPAVLLIDEVDKADIEFPNDLLRELDRMEFYVYETQKLVKARHRPAIIITSNN
                     EKELPDAFLRRCFFHYIKFPDKEIMERIVDVHFPNLKKSLLREALEVFFEIREVPGLK
                     KKPSTSELLDWLKLLMAEDISPETLRGQDQAKIIPPLAGALLKNEQDVHLFERLVFMS
                     RRAR"
     gene            1401..2579
                     /locus_tag="VE030_08275"
     CDS             1401..2579
                     /locus_tag="VE030_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZF23447.1"
                     /translation="MLTDFFLKLREGGVPASVKEYLTLMEALARHVTSGSLDEFYYLS
                     RACLVKDESNYDKFDRVFGAYFKGITSVPQQLLAQIPEEWLRRQAELLLSEEEKKLVE
                     SLGGWERLMETLKTRLEEQKGRHQGGSKWIGTGGTSPFGAYGYNPEGIRIGQEESRNR
                     RAVKVWDQREYRNLDDSVELGARNIKVALKRLRKFAREGAAEELDLDDTIRSTAKNAG
                     WLDIKMVPERHNSVKVLLFLDVGGSMEEHVKVCEELFSAARAEFKHLEYFYFHNFLYE
                     SAWRNNRRRNSERTPTFDVLHKYAHDYKLIFVGDATMSPYEIAYPGGSIEHNNEEAGM
                     AWLKRMLDVYHHAVWLNPTSEERWGYYESIGMVRQLMADRMFPLTLDGLGRAMRSLAR
                     "
     gene            complement(2533..7824)
                     /locus_tag="VE030_08280"
     CDS             complement(2533..7824)
                     /locus_tag="VE030_08280"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZF23448.1"
                     /translation="MNQLLEALRELPAPSPYRYAVRITGLALAYFVAARIGLEYAVAN
                     PVISTVWPASGIALAVVLLGGLRLLPAVFIGAFAVNCSIHGAITVSAITGLGNSLEVL
                     LGALLLRRVLDFDVRVSRLRDVVCLIVVAATIAPLPAALLGPFSLTLFGGLAWPEFIH
                     AFRIWWMGDSLGIVVVAPLLLTWVARPALDWVSPRFWYVTILLAIQFAVCYAVFGGLL
                     VNDFGLSRVTYFVLPFAVWIALAHDIRFTALANAGLFAIAVWGTAHGTGPFAGVSMEV
                     DLVLLHIFLVVYSITTLLVAAVNSERNRAVQDADSNAQRFRSLSELSSDWYWEQDQNL
                     RFTSISGSTNDRAGLSGAQSFGKTRFELPNEFESEEVRRYHAADLNARRPFRDLVLKR
                     FRPDGEVHYALISGQPIFDTHGAFMGYRGVGRDITALKLAELALRENEERLRLQFQDM
                     PIACIIHDKSFLITDWNPAAERIFGYTREEVIGRSPYDLMIPESARPDVAGRILILQQ
                     GSSPSADGVNENITKNGDTIICKWHNTVLSNADGELRGYMAMAEDVTAMVRAERAIRE
                     SEERFRALTALSSDWYWEQDEELRFSFLSSTVADATGVPADQRIGMRRWEIPGLVFDE
                     EQLKAHQELLAQRRPFQNFVYRRVMPDGSIIFVSATGSPIFDAAGDFKGYRGIGRDVT
                     ADVLIEQRVARLRDFYAALSKANEAIIHARDEQRLFDEICEVAVTHGRTIFARICLIN
                     ADTGFLDNVAVFGDHQGYPYNTSISVRTDIKEGQGPAGVALRTGIPYVGNDIQADDKN
                     HFWLPAMLATGARAMVVFPLRRGGEVVGSLHLYAAEKNWFDEELVALIGELVANISFA
                     LDNFERERARQRTEAALKESEERFRSLTDLSNDWYWEQDEHYRNTYMSPRYSARTGMA
                     MKSTLGETRFETNNVWESEEAKREHRKVLDAHLPFRDLQLARRDENGELHFMLISGQP
                     VFGPAGKFTGYRGVGKDITAQKTAEIRVARLRDFYAVLSEVNNAIIHAQDRSQLFAEV
                     CKAVAEHGRLQFVRIAMLDPSNQQVRTVAHSGDDHGLSARFYFSMDPNVPEGRAPSAR
                     ALREGHHFICNNVAEDKAGILGRELLLAAGLRSVATFPLPVGDAPAGALHLYATEPGF
                     FDDELVNLIDELAQNVSFALNNFEREAARRAAEKALRDSEQRFRDIAQAAGEFVWEND
                     NSGRFTYLSPRVVEVMGHSAEELVGHSAAEFMPAEEPERVRQWFADNMKPDRSFRDLE
                     HKIVSKSGDTLWLHISGVATSDERGNPTGHRGTARDVTEIKESEARISYLATRDPLTE
                     LPNRLLFNDRLEQGIINARRKVDALAVLFIDLDHFKNINDSLGHHVGDLLLKEVSSQM
                     TACIRKGDTLSRLGGDEFVVTLEGLSQAEDAAQVALKIIRALAKPLNVGGHMLNTTCS
                     IGISIFPNDAEDGRELMKNADTAMYHAKDRGRNNFQFFSPEMNVRAVERHQLESEMRR
                     ALELNQFVLHYQPQADMRTGKLVGMEALIRWHHPERGLVPPSSFIPVAEESGLIELIG
                     RWALKTACEQNREWQASGLPSLKVAVNISTRQFNDPRGFANEITRLLNATGLDPRYLE
                     LEMTESMLLKHVDENIAVLRRLGKLGTRLAVDDFGTGYSSLAYLKQLPIDTLKIDRTF
                     VRDIETDADDAAIIGAIIAMAHGLDVRVTAEGVETKGQMEALRKLGCDEFQGYLLSKP
                     LPAGEFTAQFLAPATKKVRRAAASARASASRGRARRA"
     gene            complement(7999..9822)
                     /locus_tag="VE030_08285"
     CDS             complement(7999..9822)
                     /locus_tag="VE030_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HZF23449.1"
                     /translation="MTPTSTLHKADQPQQQSDKRLTLEQLLADLVADKLVAQDAADKL
                     LALRRNFRSSDIHPLVMLADQKWQDPRNPKKLLNLEMLTEWLAGKVQLPYLHIDPFKI
                     DFAAVTKVMSNAYATRFRILPVGVTSTEALIATCEPYVREWETELGRILRLEIKRVLA
                     NPVDINSYLVEFYNLARSVKGAGQQHGTLLSDITNFEQLMQLGASGKVDANDQHIVRL
                     VDWLLQYAFDQRASDIHVEPRRDLANVRFRIDGVLHQVYQIPTPIVAAMTSRIKILGR
                     MDVVERRRPQDGRIKTRTADGEEIELRLSTMPTAFGEKLVMRIFNPDVLVKSFKDLGF
                     SDEDRERWNQVTSRPHGIVLVTGPTGSGKTTTLYSTLKQVASPDVNVCTIEDPIEMVE
                     PQFNQMQVQHGIGLDFATGVRTLMRQDPDIIMVGEIRDMETAEMAIQAALTGHLVFST
                     LHTNDAPSALTRLLDIGVPPYLLHGTILAAMAQRLVRTLCPLCREAGTIDDSAWNMLI
                     APWRSDKPKQVYNAKGCLECRMTGYTGRIGVYEILLMTAGQRPLVTAETDAALIREQA
                     YRDGMKPLRVAAALKVAAGVTTVDEVLKVTPPIAGERRKKS"
     gene            10226..10830
                     /locus_tag="VE030_08290"
                     /pseudo
     CDS             10226..10830
                     /locus_tag="VE030_08290"
                     /inference="COORDINATES: protein motif:HMM:NF013104.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
BASE COUNT         2038 a         3246 c         3446 g         2100 t
ORIGIN      
        1 ggccgacttt cgccggttcg caggccatgc cccggtctcc agccgcgaag tcgccgggcg
       61 gcatgttgaa caggatcatc tgcaggttgt gcttgtcgag ttgctccttg atttgcgcgg
      121 ctggaaattc gtaagggaac aggaattcga caccgcggaa tccggccttc gcggcggcgc
      181 caaagcgctc caggaagctg acctcattga acatcatcga cagattggcg ttgaaattgg
      241 gcattcatcc tcccggattt tttagtagca ggctggcggg agcggcaatt atagccgccc
      301 gcaggacggg cgcccgcgcg cggctataat ccgcaaggcg aaccggcgga aagcgagatt
      361 accgatgaag ttcaagggaa ccgagaccta cgtcgccacc gaggacctga tgatggcggt
      421 gaacgcagcc gtcacgctgg agcgcccgct gttgatcaag ggcgagccgg gcaccggcaa
      481 gaccatgctg gcgatcgagg tggcaaaggc gctggaacgg ccgctgtacc agtggcacgt
      541 caagtccacc tccaaggcac agcaggggct gtacgaatac gacgcggtgt cgcggctgcg
      601 cgattcccaa ctgggcgacg ctcgcgtaca cgatatcgcc aattacatcg tcaggggacc
      661 gctgtgggat gccttcgagt ccgaccgccc cgcggtgctg ttgatcgacg aggtggacaa
      721 ggcggacatc gagtttccca acgaccttct gcgcgagctc gaccgcatgg agttctacgt
      781 ctacgagacc cagaagctgg tcaaggcgag acaccggcct gcgatcatca tcaccagcaa
      841 caacgagaag gagttgccgg acgccttcct gcgccgctgc ttcttccact acatcaagtt
      901 cccggacaag gaaatcatgg agcgcatcgt cgacgtgcat ttcccgaacc tcaagaagtc
      961 gctgttgcgc gaggcgctgg aggtgttttt cgagattcgc gaggtgcccg gactgaagaa
     1021 gaagccgtcc acgtccgagc tgctcgactg gctgaagctg ctgatggcag aggacatctc
     1081 tccggagacg ctgcgcggcc aggatcaggc caagatcatc ccgccgctcg ccggcgcgct
     1141 gctcaagaac gagcaggacg tgcatctctt cgaacggctg gtcttcatgt ctcgccgcgc
     1201 gaggtaaatc agtccaaacc tgtttaccac gaagggcacg aagagacacg aaggaaatcg
     1261 aaaactcaat gcatgtgaaa agaatgcaac tacgagaaat tgggaatatt cgccggttga
     1321 ttggacttga attgtccgcg ctggcagttt tgggtttcct tcgtgtctct tcgtgtcctt
     1381 cgtggtgaaa gaagctttcc ttgctgactg acttttttct gaagctgagg gaaggcgggg
     1441 tacccgcgtc ggtgaaggaa tacctgaccc tgatggaggc gctcgccagg cacgtcacgt
     1501 ccggcagcct ggacgagttc tactatcttt cgcgcgcctg cctggtgaag gacgagtcga
     1561 actacgacaa gttcgaccgc gtcttcggcg cctatttcaa gggcatcact tcggtgccgc
     1621 aacaattgct ggcgcagatc cccgaggaat ggctgcgccg gcaggcggaa ctgttgctct
     1681 ccgaagaaga aaaaaaactg gtcgaatcgc tcggcggctg ggagaggctg atggagacgc
     1741 tgaagacgcg gctcgaggag cagaagggcc gccaccaggg cggctcgaag tggatcggta
     1801 ccggcggcac gtcgccgttc ggcgcctacg gctacaaccc ggaaggcatt cgcataggcc
     1861 aggaggagtc gcgcaaccgc cgcgcggtga aagtgtggga ccagcgcgag taccgtaatc
     1921 tcgacgattc ggtcgaactg ggcgcgcgca acatcaaggt tgcgctcaag cgcctgcgca
     1981 agttcgcccg tgagggcgcg gccgaggaat tggatctgga cgacaccatc cgctcgaccg
     2041 cgaagaacgc cggctggctc gacatcaaga tggtgccgga gcggcacaac tcggtgaagg
     2101 tgctgctgtt cctggacgtc ggcggttcga tggaggagca cgtaaaggtg tgcgaggaac
     2161 tgttctcggc cgcgcgcgcc gagttcaagc acctcgagta cttctacttc cacaacttcc
     2221 tctacgagtc ggcgtggcgc aacaatcgca ggcgcaacag cgaacgcacg ccgactttcg
     2281 acgtgctgca caagtacgcc cacgattaca agctgatctt cgtgggcgat gcgaccatga
     2341 gtccgtacga gatcgcctac ccggggggca gcatcgaaca caacaacgag gaggcaggga
     2401 tggcgtggct gaagcgcatg ctggacgtct accaccatgc ggtgtggctc aatccgacct
     2461 cggaagagcg ctggggctac tacgaatcga tcggcatggt ccggcaactg atggccgacc
     2521 ggatgttccc gctcacgctc gacgggctcg gccgcgcgat gcgctcgctc gcgcgctagc
     2581 ggcggccctg cggactttct tcgtagccgg cgcgaggaat tgcgcggtga attcccctgc
     2641 gggtagaggc ttggacagca gataaccctg gaattcgtcg catcccagct tgcgcagcgc
     2701 ttccatctgt cccttggtct ccactccttc cgcagtcacg cgcacatcca gcccgtgcgc
     2761 catcgcgatg atggccccga taatggcagc gtcatccgcg tccgtctcga tgtcccgcac
     2821 gaaggtgcgg tcgatcttca gggtatcgat cggcaactgc ttgaggtagg cgagcgagga
     2881 gtacccggtg ccgaagtcgt ccaccgcgag gcgggtgccg agtttaccca gccgccgcag
     2941 caccgcgatg ttttcgtcca cgtgtttcaa cagcatgctc tcggtcatct ccagctcgag
     3001 gtaacgcgga tccagtccag tggcattgag caagcgcgtg atttcgttgg cgaaacctct
     3061 cggatcgtta aactggcggg tggagatgtt gacggccacc ttcaggctcg gcaagccgct
     3121 cgcctgccac tcgcggttct gttcgcaggc ggtcttgagc gcccaccggc cgatcagctc
     3181 gatcagcccc gattcttccg cgacggggat gaagctcgac ggcggcacca ggccgcgctc
     3241 aggatggtgc cagcggatca gcgcctccat tccaacgagt ttcccggtgc gcatgtccgc
     3301 ctgcggctgg tagtgcagga cgaactggtt caattccaac gcccgccgca tttcgctttc
     3361 gagctgatgg cgttccaccg cgcgcacgtt catttccggg gagaagaact ggaaattgtt
     3421 gcgcccgcga tccttggcat ggtacatggc ggtgtccgca tttttcatca gttcgcggcc
     3481 gtcctcggca tcattgggga aaatgctgat gcctatgctg caggtggtgt tgagcatgtg
     3541 cccgccgaca ttcagcggct tggcaagcgc ccggatgatc ttgagcgcaa cttgggccgc
     3601 gtcctcggcc tggctcaggc cctcaagggt gacgacaaac tcgtcgccgc ccaggcgcga
     3661 caaggtatcg cccttgcgta tgcatgcggt catctggctg gagacttcct tgagcagcag
     3721 gtcgccgacg tggtggccga gcgaatcgtt gatgttcttg aagtggtcca ggtcgatgaa
     3781 cagcaccgcg agcgcatcga ccttgcggcg tgcgttgatg atcccctgtt cgagacggtc
     3841 gttgaacagc aggcggttgg gcagctcggt cagtggatcg cgggtggcga ggtagctgat
     3901 gcgggcctcc gattccttga tttcggtcac atcccgcgcc gttccccggt ggcctgttgg
     3961 attgccgcgc tcgtccgagg tcgcgacccc gctgatgtgc agccacagag tatcgccgga
     4021 cttggagacg atcttgtgct ccaggtcgcg gaaggagcgg tccggcttca tgttgtcggc
     4081 aaaccactgg cgcacccgct ccggctcctc cgcgggcatg aactcggctg cggaatggcc
     4141 gaccagttcc tcggcgctgt gacccatgac ttcgactacc ctgggggaga ggtaggtgaa
     4201 acggccggag ttgtcgttct cccatacgaa ctcgcccgcg gcctgggcga tgtcgcggaa
     4261 ccgctgttcg ctgtcccgca gcgctttttc cgcggcccgg cgtgcggcct cgcgctcgaa
     4321 gttattgagg gcaaacgaga cgttctgagc gagctcgtcg atgagattga ccagttcgtc
     4381 gtcgaaaaat cccggttcgg tcgcgtacag gtgcagggcg ccggccgggg cgtctccgac
     4441 cggtagcggg aaagtcgcaa cagaacgcag gcccgccgcg agcagcaatt ctctgccgag
     4501 aatgccagcc ttgtcttcgg caacgttgtt gcaaataaag tgatggcctt cgcgcagcgc
     4561 ccgcgccgaa ggcgcccggc cttcgggaac gttcgggtcc atcgaaaaat agaagcgtgc
     4621 cgacagtccg tggtcatcgc cgctgtgcgc gaccgtgcga acctgctggt tcgaggggtc
     4681 aagcatggca atacgtacga attgaagccg gccgtgttcg gccacggcct tgcagacctc
     4741 ggcgaagagc tggctgcgat cctgagcgtg aatgatcgcg ttgttgacct cgctcagcac
     4801 ggcataaaag tccctgagac gcgccacgcg gatttcggcg gtcttctggg cggtgatgtc
     4861 cttgcccacg ccgcggtagc cggtgaattt cccggcgggc ccgaaaaccg gctggccact
     4921 gatcagcatg aagtgcaatt cgccgttctc gtccctgcgc gcgagctgca ggtcgcggaa
     4981 cgggaggtgg gcatcgagca ccttccgatg ctcgcgcttg gcctcctcgg attcccatac
     5041 gttgtttgtc tcgaagcgcg tctcgccgag cgtggatttc atcgccatgc cggtcctggc
     5101 gctgtagcgc ggcgacatgt acgtattgcg gtagtgctcg tcctgctccc agtaccagtc
     5161 attcgacagg tcggtcaggc tgcggaagcg ctcctcgctc tccttgagcg ccgcttcggt
     5221 gcgctgccgc gccctttcac gctcgaagtt gtcgagcgca aaggaaatgt tggccaccag
     5281 ctcgccgata agcgcgacca gttcctcgtc gaaccagttc ttttccgctg cgtacaggtg
     5341 caacgagccg acgacttcac cgccgcgtcg gagcggaaac accaccatcg ctcgtgcgcc
     5401 ggttgccagc atagctggca gccagaagtg gttcttgtcg tcggcctgga tgtcgttgcc
     5461 gacgtacggg atgccggtgc gcagcgcgac cccggccgga ccctgacctt cctttatatc
     5521 tgtccgcacc gaaatgctgg tgttataggg gtagccctgg tggtcgccga atacggcgac
     5581 gttgtcgaga aaaccggtgt cggcattgat caggcagatg cgcgcaaaga tggtgcggcc
     5641 gtgcgtgacc gcgacttcgc aaatttcgtc gaacagccgc tgctcgtccc tggcgtggat
     5701 gatcgcttca ttggccttgg acagggcggc gtagaaatcg cgaaggcgcg cgacccgctg
     5761 ctcgatcagc acgtccgcag tgacgtccct gccgatgccg cgatagccct tgaagtcgcc
     5821 ggcagcatca aatatcggcg acccggtggc cgagacgaaa atgatcgagc catcgggcat
     5881 gacgcgcctg tagacgaagt tctggaacgg cctgcgctgg gcaagcaatt cctggtgcgc
     5941 cttcagttgc tcctcgtcga acaccaaccc cggaatctcc cagcggcgca tgccgatgcg
     6001 ttgatctgcg ggcactccgg tggcgtcggc gaccgtgctc gacaggaaag aaaagcgcaa
     6061 ttcctcgtcc tgctcccaat accagtcgga tgacagcgcg gtcaacgcgc ggaaacgctc
     6121 ctcgctttcg cggatcgcgc gttccgcccg caccattgcg gtgacgtcct ccgccatcgc
     6181 catgtatccc ctgagttctc cgtcggcgtt cgacagaacc gtgttgtgcc acttgcaaat
     6241 gatcgtgtcg ccgttcttgg tgatgttctc gttgacgccg tcggcggagg gggaggagcc
     6301 ttgctgcaga atcaggattc ggccggccac gtcggggcgt gcgctttcag gaatcatcag
     6361 gtcgtagggc gagcgtccga tcacctcctc ccgggtatag ccgaagatcc tctctgcggc
     6421 ggggttccag tcggtgatca ggaaggactt gtcgtggatg atgcaggcga tcggcatgtc
     6481 ctgaaactga agcctcaggc gctcttcgtt ctcccgcagc gccaattcgg cgagcttgag
     6541 ggcagtgatg tcgcgaccga cgccgcgata acccatgaat gcgccatgcg tgtcgaaaat
     6601 cggctggccg ctgatcaagg cgtagtgcac ttcgccatcc ggcctgaagc gcttcagcac
     6661 cagatcgcgg aaggggcgtc gcgcattcag atcggctgcg tggtagcggc gaacctcctc
     6721 ggattcgaat tcgttgggaa gttcgaatcg cgtcttgccg aatgactgcg ccccggaaag
     6781 cccggcccgg tcgttggtcg acccggaaat cgaagtaaat cgcaggttct ggtcctgctc
     6841 ccagtaccaa tccgaggaga gttccgacag gctccggaat cgctgggcgt tgctgtctgc
     6901 atcctggact gcgcgattgc gttcgctgtt caccgcagcg accaggaggg tggtgatcga
     6961 gtacacgacc aggaaaatgt gcagaagaac gagatcgact tccatcgaaa cgccggcgaa
     7021 aggaccggtg ccgtgggcag tcccccaaac ggcgattgcg aacaggccgg cgttggcgag
     7081 cgcggtgaag cggatgtcgt gggcaagcgc gatccatacc gcgaacggga gcacgaagta
     7141 ggtcacgcgg ctcaatccga agtcgttgac gaggagcccg ccgaacactg catagcacac
     7201 cgcaaactga atcgcgagca ggatcgttac gtaccagaat cgaggtgaga cccagtcgag
     7261 tgcaggcctc gccacccacg tcagaagcaa aggcgcgacc acgacaatgc ccagcgagtc
     7321 ccccatccac caaatgcgaa aggcgtgaat gaactcgggc caagcgaggc cgccgaacag
     7381 cgtgagactg aacggtccca gcagtgcggc cggcagcggt gcgattgtcg ccgccacgac
     7441 gatcaggcag accacgtcgc gcagcctgga cactcgcacg tcaaaatcga gaacgcggcg
     7501 cagcagcaag gcgccaagca gaacttccag cgaattgccg agtccggtga ttgcggagac
     7561 ggttatggct ccatggatcg agcagttcac ggcaaatgcg ccgatgaata ccgcgggcag
     7621 aagccgcaag cctcccagca gcaccacagc cagtgcgatg cccgaagcgg gccaaaccgt
     7681 ggaaatgacc ggattggcga cggcgtactc aagcccgatg cgcgcagcga cgaagtacgc
     7741 cagcgccagc ccggtgatgc gaaccgcgta gcggtacggc gagggagcag gaagctcccg
     7801 cagcgcttca agcaattgat tcatgactac cgcgggagag gatgaccggg tccggtggac
     7861 ggatggctga tacggacgcg cgcaggcagg tctcagaaaa ccgacgcaac acgagcagcc
     7921 ttgccccgca ctttcgtatt gaaacgtgcc gcgtcgatca cgagtggacg gtcctcttgg
     7981 ggatcaggtg aggcggaatc aggatttttt cctgcgctca cctgcaatcg gtggcgttac
     8041 cttgagcacc tcgtccaccg tcgtaacgcc ggcggcgact ttgagcgcgg cggcgacccg
     8101 cagcggtttc atgccgtcac ggtacgcctg ctcgcgtatc aacgcggcat cggtctcagc
     8161 cgtgacgagc ggccgctgtc ccgcggtcat cagcaggatt tcatagacgc caatccggcc
     8221 cgtgtaaccc gtcatccggc actcgaggca gcccttggcg ttgtaaacct gtttcggttt
     8281 atcagatcgc cacggcgcaa tcagcatgtt ccaggcgctg tcgtcgatag tgccggcctc
     8341 ccggcaaaga ggacagagcg tgcgcaccag ccgttgcgcc atagccgcca gaatggtgcc
     8401 gtgaagcaga taaggcggca cgccgatgtc gagcaggcgg gtgagcgcgg agggcgcgtc
     8461 gttggtgtgc agtgtggaaa acaccagatg gccggtgagc gccgcctgga tcgccatttc
     8521 cgcggtttcc atgtcgcgga tctcgccgac catgatgatg tcggggtcct ggcgcatcag
     8581 cgtgcgcaca ccggtggcaa agtcgagccc gatgccgtgt tgaacctgca tctggttgaa
     8641 ctgcggctcg accatttcga tggggtcctc gatggtgcac acgtttacat ccggcgaggc
     8701 cacctgtttc agggtcgagt acagcgtggt ggtcttgccg gagccggtcg gcccggtcac
     8761 cagcacgatg ccgtgaggac gcgatgtaac ctggttccag cgctcacgat cctcgtcgga
     8821 gaagcccaga tccttgaagc tcttcaccag cacgtccggg ttgaatatcc gcatcaccag
     8881 tttctcgccg aaggccgtgg gcatggtgga caggcgcagc tcgatctctt cgccatccgc
     8941 agtgcgcgtc ttgatgcggc cgtcctgtgg ccggcgcctt tccacgacgt ccatgcgccc
     9001 gaggatcttg atgcgactgg tcatcgcggc gacgatgggc gtggggatct gatacacctg
     9061 gtgcagcacg ccgtcgatgc ggaagcggac gttggccagg tcgcggcgcg gttcgacatg
     9121 gatgtcgctg gcgcgctggt caaaggcata ttgcagcagc cagtcgacca ggcggacgat
     9181 gtgctggtcg ttggcatcga ccttgccgct tgcgcccagc tgcatgagct gttcgaagtt
     9241 ggtgatgtcg gaaagcagtg tgccgtgctg ctggcctgcg cccttgaccg accgcgccag
     9301 gttgtagaac tcgaccaggt agctgttgat gtcgaccgga ttggccagca cacgcttgat
     9361 ctccagcctc aggatgcggc cgagctcggt ttcccattcg cgtacatacg gctcgcaggt
     9421 ggcgatcagc gcttcggtcg aagtcacgcc gaccggaagg atgcggaagc gggtggcata
     9481 ggcgttcgac atcaccttgg tgaccgcggc gaaatcgatc ttgaacggat cgatgtgcag
     9541 gtacggcagc tgcaccttgc cggccagcca ctcggtgagc atctccagat tcaggagttt
     9601 cttggggttg cgtggatctt gccacttctg gtcggcaagc atcaccagcg ggtgaatgtc
     9661 gctgctgcgg aagttgcggc gcagcgccag cagcttgtca gccgcgtctt gggccaccag
     9721 tttgtcggcg acaaggtcgg cgagcaattg ctcgagagtg agcctcttgt cgctctgttg
     9781 ctggggctgg tcggctttgt gcagggtcga ggtcggcgtc atgcccttat ctggagaagt
     9841 ggaagtgggc tgtcagggga ggcaatgtcg cctccaccga cgacggtgtc gcgcttgcgt
     9901 tgcactataa accgcctgtc tgcggcctcc aacacagact ggaccgtgga acccgaagcc
     9961 cgtccgggga gcgcgcccgg gaatacgtat gaacttcaat tgtgcctatt ttccgccaca
    10021 tgcaccatac cggttcctaa aggcgttgcc ggtgcttgat cacaccctta aagccgttat
    10081 cgtacagaca gttgtttttt tcggaatgag ggttgaatga agggatccga tttcacctgc
    10141 aagaacatgg gaggggagca tgaaagccct gaaaatcagc agtgacgcgc tgttcattct
    10201 gcttggcgcg gcgatggtgc tggcgatgca cgccgggttc gcgtttctgg aagtcggcac
    10261 cgtgcgcaag aaaaaccagg tgaatgcgct agtgaagatc ctcgcggatt tctccgtatc
    10321 caccgtggcg tatttcttca tcggctattc gctcgcctac ggcgtcaact tcttcgcggc
    10381 ggcacacaag ctcgcgacag acagcgggta tcaactggtc aagttctttt tcctgctgac
    10441 ttttgccgcc gccaccccgg caatcgctcc gggggcatcg ccgaacgcgc caagttcaac
    10501 tcgcaactgg cggcgacttt ccttctggtc ggattcatct atcccttctt cgaaggcatc
    10561 tcctggaacg gcaactacgg cgtgcaggga tggctggagg ccaccttcgg cgcgccgttt
    10621 catgactttg ccggttcgat cgtggtgcat gccgtaggcg gctgggtcgg gctggtcgcg
    10681 gtcatcctgc tcggtgcccg ccggggacgc tacaaccgcg aagggggcat ctccgcgcac
    10741 ccgccgtcga gcattccatt cctcgcactc ggtgcctggg tgctcaccgt gggctggttc
    10801 ggcttcaatg tcatgtctgc actgcagatt
//
LOCUS       DASQOP010000203         2017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2906658, whole genome shotgun sequence.
ACCESSION   DASQOP010000203 DASQOP010000000
VERSION     DASQOP010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2906658"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VE030_08295"
     CDS             complement(<1..908)
                     /locus_tag="VE030_08295"
                     /inference="COORDINATES: protein motif:HMM:NF016593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HZF23450.1"
                     /translation="MTEQEFKQLAAQGFNRIPVVLETFADLDTPLSVYLKLANKPYSY
                     LLESVQGGERFGRYSFIGLPAETRFEVRGHTCAEYRRGILVSQAEHDNPLDFVSACHA
                     RIRAASRPELPRFLGGLVGCFGYDTVRYVEKKLAATRKADTLHTPDILLLLSEQLVVL
                     DNLSGKLFLVVYADPREAGAYSRAQQRLQELLWMLRQPVVVPPEAPTAPSQPQSEFSE
                     DAFIEAVERAKRYIFDGDIMQVVLSQRMSLPYHASPLSLYRALRALNPSPYMFYFDMG
                     GFHVVGASPEILVRLESGMVTLRPIAG"
     gene            complement(1043..1729)
                     /locus_tag="VE030_08300"
     CDS             complement(1043..1729)
                     /locus_tag="VE030_08300"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: protein motif:HMM:NF009695.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HZF23451.1"
                     /translation="MRFPIEIKAIAFDLDGTLVDTLPDLHESANRMLADLGRPAVAES
                     AVRAYVGDGVDRLVRRLLTGDADGEPDTALFDRAGNAFRRHYASVLSRASRPFPGVVE
                     GLQEMRRRGLTLACITNKPSGFTDPLLRDIGLAPYLDLVLSGDSLPRKKPDPLPLLHC
                     AQVFGVPASRLLMIGDSPNDTRAARAAGCPVFCVPYGYRGTLGLHELDCDAIVPALLD
                     SMKLVEISRS"
     gene            complement(1900..>2017)
                     /locus_tag="VE030_08305"
     CDS             complement(1900..>2017)
                     /locus_tag="VE030_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HZF23452.1"
                     /translation="EIARAGADTFVAGSAIFGTKDYKATITAMRSELAKASP"
BASE COUNT          413 a          625 c          639 g          340 t
ORIGIN      
        1 ccggcaatcg gacgcagcgt gaccatgccg ctttccagcc gcaccaggat ctcgggcgag
       61 gcgccgacca cgtgaaatcc gcccatgtcg aaatagaaca tgtaaggcga ggggttgagc
      121 gcacgcaggg cacggtacag cgacaaaggc gaggcatggt aaggcaggct catgcgctgc
      181 gacagcacca cctgcatgat gtcgccgtcg aaaatgtagc gcttggcgcg ctccaccgcc
      241 tcgatgaatg cgtcttcgct gaactcggac tgaggctgtg acggcgccgt gggcgcttcc
      301 ggcggcacca ccaccggctg gcgcagcatc cacagcagtt cctgcaggcg ctgctgggca
      361 cgcgaatacg cgccggcttc gcgcggatcg gcatagacca ccagaaacag ctttccggaa
      421 agattgtcca gcaccaccag ttgctcggag agcagcagca ggatgtccgg ggtatggagg
      481 gtgtcggcct tccgcgtggc ggcgagcttc ttttccacgt aacgaaccgt gtcgtagcca
      541 aaacagccga ccaggccgcc gagaaagcgc ggcaactccg gccgggacgc cgccctgatg
      601 cgcgcatggc aagccgagac gaaatcgaga ggattgtcgt gctcggcctg gctcaccagt
      661 atgccgcgcc ggtactcggc acaggtatgg ccgcgcacct cgaagcgggt ctccgcgggc
      721 aagccgatga aggagtagcg gccgaaacgc tcgccgccct gcaccgattc cagcagatag
      781 gaatagggct tgttggccag cttgagataa acagaaagcg gggtgtcgag atcggcaaag
      841 gtttcgagga caaccgggat gcggttgaaa ccctgcgcgg caagctgctt gaattcctgc
      901 tcagtcatga cgaactcctg aaactgcgaa agacggtgaa acggaaaccg cgacgctcag
      961 atgcagcgcc agagcacgga ccggcgccac cagcgccagc cggctccgat ttccagcctc
     1021 aggttttcga gtttcaaaaa gttcatgacc gtgaaatttc gaccagtttc atggagtcca
     1081 gaagcgccgg tactatagca tcgcagtcca gttcgtgcag cccaagcgtg ccccggtagc
     1141 catagggcac gcagaacacc gggcaaccgg ccgcccgcgc cgcccgggtg tcgttggggg
     1201 aatcgccgat catcagcagg cgcgacgccg gcacgccaaa gacctgtgcg caatggagca
     1261 gcggcaatgg atcaggcttt ttgcgcggca ggctgtcccc gctcaatacc agatccagat
     1321 acggcgcgag cccgatatcg cgcagcagcg ggtcggtgaa ccccgacggc ttgttggtaa
     1381 tgcaggcgag cgtgagcccg cggcggcgca tttcctgcag cccctccacc accccgggaa
     1441 agggccgtga tgcacgcgac agcacacttg cgtagtgacg gcggaaggcg tttccggccc
     1501 gatcgaacag tgctgtatcc ggttcgccgt ctgcatcgcc ggtcaacagg cgtctgacca
     1561 ggcggtccac cccgtctccc acatacgccc gcaccgcgga ttcggccacc gcggggcgcc
     1621 cgaggtctgc gagcatccgg ttggccgatt cgtgcaggtc cggcagcgta tcgaccagcg
     1681 tgccgtccag atcgaacgct atcgctttaa tctcgatcgg gaatctcaaa gaaaactctc
     1741 accgccaagg acgcaaggga aagacgaaaa gggttatctc acggtgtcga aatcttttgt
     1801 tgatggttat gccggcgggc tttcctcgat gcacccctat cggacaggat ttgccgtgat
     1861 cgcctatcct tggcgtcctt ggcggtccgc tctggaactt tacggggacg ctttcgcaag
     1921 ttcggagcgc atggcggtga tggtggcctt gtagtccttg gtgccgaaga tcgcagatcc
     1981 ggcgacgaaa gtgtccgccc cggcgcgggc aatctcg
//
LOCUS       DASQOP010000204        14554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2907011, whole genome shotgun sequence.
ACCESSION   DASQOP010000204 DASQOP010000000
VERSION     DASQOP010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14554
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2907011"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..222)
                     /locus_tag="VE030_08310"
     tRNA            complement(136..222)
                     /locus_tag="VE030_08310"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(186..188),aa:Leu,seq:cag)
     gene            299..2527
                     /gene="rnr"
                     /locus_tag="VE030_08315"
     CDS             299..2527
                     /gene="rnr"
                     /locus_tag="VE030_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HZF23453.1"
                     /translation="MKTNKKTEIVSRKKSSAQARGTDVRSNDPHLEREQSRYEHPLPS
                     REYILQVLLEQGVPVAEDRLAQLLGVAPHEMDAFVRRLAAMERSGQIMRNRKNAICVV
                     EKLDLVNGRVQGHPDGFGFLIRDDRGEDLFLNPGEMSKVLHGDRVVAREGGVNARGRR
                     EGQIVEVLERANQKVVGRFLNEHGIGVVAAEDRRISQDILIPAGATGGAKPGQVVVAE
                     IIEQPKPHARPIGRIVEVLGNYADPGMEIEIALRKHDLPHVFSKEIEKVAAKFPAGVT
                     EKDLKGREDLRNLPLVTIDGETAKDFDDAVYAERNGKGFKLWVAIADVSHYVQHGDPI
                     DLEARLRGNSVYFPRRVIPMLPEVLSNGLCSLMPKVDRLCMVCEMDISARGDIGAYRF
                     YPAVMHSRARLTYTQVAKVLEDPQGEPTRELRQLVPQLQTLYALYQGLTKARALRGAI
                     DFETVETQMFFDGQGKIERIVPTERNDAHRLIEECMLAANVSASDFLSGHDHPALYRV
                     HEGPTPEKLAALREFLREFGLQLTGGDQPQAKDYARLLEKIKTRPDVQLLQTVMLRSL
                     RQAIYSPDNVGHFGLAYESYTHFTSPIRRYPDLVIHRSIKAVLAGKRYDPGDWHEIGV
                     HCSETERRADEATRDVEAWLKCYYMQDRISERFTGTVSGVTSFGVFVALDQVYVEGLV
                     HISELGSDYFHYDAAKHQLLGERTGKRFRLGDRVSVKLVRVDLETSKIDFVLDEPKSK
                     GK"
     gene            2524..3285
                     /gene="rlmB"
                     /locus_tag="VE030_08320"
     CDS             2524..3285
                     /gene="rlmB"
                     /locus_tag="VE030_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HZF23454.1"
                     /translation="MSGRRAIHGLHAVTAALRRRPEAVFEVFLDPGRNDPRARRVADL
                     AKAGSVRVHHADAARLDGLAKGSRHQGVVAFVEESAPAGSLEEVLESATEPPLLLVLD
                     GVQDPHNLGACLRVADAMGVHAVIAPKDRAAGLSATVHKVASGAAESVPFIVVTNLAR
                     ALRDLKDKGIWVLGAAEAGATTLFQSRLSGALALVLGAEGEGLRRLTRETCDELIAIP
                     MLGQVESLNVSVAAGICLYEARRQRHAATGGAVNP"
     gene            3496..4242
                     /gene="rpsB"
                     /locus_tag="VE030_08325"
     CDS             3496..4242
                     /gene="rpsB"
                     /locus_tag="VE030_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HZF23455.1"
                     /translation="MSVTMRQMLEAGVHFGHQTRYWNPKMAPFIFGHRSKIHIINLEK
                     TLAMYQDAVKFVRQLAANKGSILFVGTKRQAREIIREEALRAGTPFVDHRWLGGMLTN
                     FKTVKQSIKRLRDMEQMVQDGSIEKLSKKEQLNFQREMVKLDRSLGGIKEMNGLPDAL
                     FVVDVGYQKGAVTEANKLGIPVVGVVDTNHSPDGVDYVIPGNDDASRAVRLYVRGMAD
                     AILEGRSQIVQQIVSDEFVEVDSSEEQAPA"
     gene            4344..5228
                     /gene="tsf"
                     /locus_tag="VE030_08330"
     CDS             4344..5228
                     /gene="tsf"
                     /locus_tag="VE030_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HZF23456.1"
                     /translation="MAEITANMVKELRSRTGLGMMECKKALEEAGGDMNKAEDLLRIR
                     SGAKASKVAGRVAAEGTIGIHLSGDRKLGALVEVNCETDFVAKDQNFAGFAKAVAELA
                     AAGGITDVEALTRARLASGESVEATRQALVMKLGENIGVRRVAVHKAKGRLAHYLHGT
                     RIGVMVDLEGGDDTLGKDLAMHVAASKPISVSRDQVPAELIAREREIARARALESGKP
                     ANLVEKIVEGSVHKYLAEVTLLGQPFVKDDKTTVEKLLAAKGAKVHAFTLFIVGEGIE
                     KKATDFAAEVMAQAAQAR"
     gene            5249..5962
                     /gene="pyrH"
                     /locus_tag="VE030_08335"
     CDS             5249..5962
                     /gene="pyrH"
                     /locus_tag="VE030_08335"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HZF23457.1"
                     /translation="MKPAYKRILLKLSGEALMGDDAYGVNRETIDRIVSEVKEVVGLG
                     VEVGVVIGGGNIFRGMAPAAAGMDRATADYMGMLATVMNALALQDAMRRQGLKSRVQS
                     AINIEQVAEPYIRGKAMRYLEEGRVVIFAAGTGNPFFTTDTAAALRGMEMNVDIVVKA
                     TKVDGVYTDDPKTHPDAMRYQRVSFDEAIVKKLKVMDATALTLCRDQKLPICVFSIFK
                     AGALKRVVLGEDEGTLVHA"
     gene            5984..6544
                     /gene="frr"
                     /locus_tag="VE030_08340"
     CDS             5984..6544
                     /gene="frr"
                     /locus_tag="VE030_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HZF23458.1"
                     /translation="MTIADVKKSADQKMHKTLDTLKADLGKIRTGRAHTGILDHIQVD
                     YYGNPTPLNQVANVSLIDARTIGVTPWEKKMVAVIDKAIREADLGLNPATMGDTVRVP
                     MPALTEERRRDLTKVVKGEAENARVAVRNIRRDANTALKELLKQKAVSEDDERRAQED
                     IQKLTDRYIAEIDKALAAKEADLMAV"
     gene            6552..7337
                     /gene="uppS"
                     /locus_tag="VE030_08345"
     CDS             6552..7337
                     /gene="uppS"
                     /locus_tag="VE030_08345"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HZF23459.1"
                     /translation="MFTSATLAVPEVRAVPRHIAIIMDGNGRWAKKRFLPRVAGHRRG
                     LEAVRATTKASIERGVEYLTLFAFSSENWRRPKDEVSFLMELFVMALEQEVSKLHANR
                     IRLRVVGDLSSFEPRLTTLISNAEQLTAGNDRLTLTIAANYGGRWDILQAVNRMVREL
                     PNPQDAYREAHLAPHLSMSYAPEPDLFIRTGGEQRISNFLLWQLAYTEFYFTDELWPD
                     FDAEALARAIDSYRSRERRFGRTSEQLGAESAPAGTSRGTVGE"
     gene            7384..8211
                     /locus_tag="VE030_08350"
     CDS             7384..8211
                     /locus_tag="VE030_08350"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HZF23460.1"
                     /translation="MLKQRLLTVAVVLPLLLACIFLLPNPGWALLMTVPTTLGALEWA
                     KLAGYGRGARALFIAVVLASCLMLIAVAAAPRLLALAMNLATALFMLALLFWAIAVPV
                     WLYSKWRVSQPLLLAVAGWIVLVPAWLAVVRLQQSAWLLLVVLGAVWVADTAAYFAGH
                     RFGRNKLAPQISPGKTWEGVIGAYIAVLGYALAASLILQPSANGYDRLGVLLFACVLT
                     AFGIVGDLFESWIKRQAGAKDSGQLLPGHGGVLDRIDSLTAALPFAALYFIQAAWSA"
     gene            8208..9389
                     /gene="ispC"
                     /locus_tag="VE030_08355"
     CDS             8208..9389
                     /gene="ispC"
                     /locus_tag="VE030_08355"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HZF23461.1"
                     /translation="MKRLAILGSTGSIGASTLAVVERHPDRFEVTALSADRQVDRLLA
                     QCLKFRPKYAALADAAAAEQLRVEVRAAGLKCEVLAGEAALAQIAAAPEVDAVMAAIV
                     GSPGLASTLAAARAGKQLLLANKEALVMAGALLMDAVRASGAVLLPIDSEHNAIFQAL
                     PREFDGDFGRAGVKRLWLTASGGPFLRAARAQLAQVTPEQACAHPNWVMGRKISVDSA
                     TLMNKGLEVIEASLLFRAKSAQIEVVIHPQSIVHSLVEYVDGSVLAQLGNPDMRTPIA
                     HALAYPERIDPGVEALDLFAVASLGFERPDLDRFPSLRLAYRALQAGGSAPILLNAAN
                     EVAVASFLERRLAFDRIPELVEEVLERVAAAPVQSLEDVLAADSLARRTAMQRLETVT
                     H"
     gene            9397..10761
                     /gene="rseP"
                     /locus_tag="VE030_08360"
     CDS             9397..10761
                     /gene="rseP"
                     /locus_tag="VE030_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HZF23462.1"
                     /translation="MSLISTLIAFVVALGVLIVFHEFGHYLVARLCGVKVLRFSVGFG
                     MPLWKRKFGRDGTEWTIAAFPLGGYVKMLDEREGPVAAEERHRAFNVQPVAKRFAVVL
                     AGPVANFLLAILLYWLLFVHGLPGIRPVVGAPLPDTPAAHAGFHAGDLIRRIGEVQVS
                     TWQDVRWVLLENALRKEAVRVELQSERGDINFGRLDLSRIGPEHIDGDLLQSVGITRY
                     QPPLKPVIGRLIAGGAGERDGLKPGDEVVEVDGRPIAHWDELVSAVSTAAGRALTMRV
                     RRTDTVFQLTVTPEAKTEGGKTVGRIGAAPHVDEAEMKRLVTEVRYGPAAAAVKALER
                     TWEVSLFTLKMLGKMIVGELSLKNLSGPITIADYAGQSAQMGWLAYLSFLALISISLG
                     VLNLLPVPLLDGGHLMYYVAEIIKGSPVSDRVMEIGQQLGIVLLFSLMAFAIYNDINR
                     LFGG"
     gene            10768..13035
                     /gene="bamA"
                     /locus_tag="VE030_08365"
     CDS             10768..13035
                     /gene="bamA"
                     /locus_tag="VE030_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HZF23463.1"
                     /translation="MKKRLLLLALSSLVSLSTLAFEPFQVKDIRVQGIQRTEAGTVFS
                     YLPVKVGDTIDQERATSAIKALYATGFFKDVRLEQDGDVLVVVVEERPAIAQIAIAGS
                     KEFDAKQLKDGLKQIGLAESRIFDRALLERAEQELKSQYISRGKYGVTITTTVTPLER
                     NRVAINFDISEGEVAKIRQINIVGNSVFKDDKLRDEFVLRTPGWLTWYSKNDQYSKPK
                     LQGDLEALRTFYLNQGYLEFTIDSTQVSITPDKRDIFITVNITEGKKFTVTDVKLAGE
                     LIAPEAELRKLIKLKKGDVFSRERLTETTKLIGDRLGNEGYAFASINPVPEIDRDKSE
                     VAFTLYVDPGRRVYVRRIGISGNTNTRDEVIRREMRQLESGWYSTEKLNRSKQRIDKL
                     GFFSDVQIDTPGVPGTSDQVDVDVKVNERATGNLTFGVGYSSAEKVILSAGVSQSNIF
                     GSGNALSFQVNTGSINRTYALSYTNPYYTDDGVSRGFDIYKRDVNATSLAIASYKTST
                     IGGGVRFGVPVTEYDTVNYGLGYERTELELQPNTPLRYVDFVNEFGARTDTILGSVGW
                     SRDKRDSVIYTTSGTFQRITADVGLPGADLTYYRTNLQHQWYHPLTRDYTLHFNGQVG
                     YADGYDKKPLPFYKVFYLGGVNSVRGYETASIGPKDSNGDSLGGSHQLLANLEFLFPF
                     PGLAKDRSVRLSLFADAGVVGEKYDFGEMRYSTGLAFNWFSPVGPLKLSFGKALNPQP
                     DDRLQRIQFSFGTTF"
     gene            13102..13593
                     /locus_tag="VE030_08370"
     CDS             13102..13593
                     /locus_tag="VE030_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HZF23464.1"
                     /translation="MSLAIAAVALLASSNAPAAAELKIGFIKTDRVFREAAPAVRALK
                     KIEKEFAPRDQEIQKLAKQTRDLQSVLEKEAMTMSETDRRDKEQELARMTRELQRMQR
                     EFREDLNLRRNEEMAAVLERAQKVIQQIAETEKFDLILQEAVYMSPRLDITDKVIKAL
                     ADK"
     gene            13677..>14554
                     /gene="lpxD"
                     /locus_tag="VE030_08375"
     CDS             13677..>14554
                     /gene="lpxD"
                     /locus_tag="VE030_08375"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HZF23465.1"
                     /translation="MTGDVRKSFSLAELADHFGGTVVGDAATRIRQVAPLETAGPEEL
                     SFLASRKYQKLLASTRARAVILGEEARDATALPRIVCKYPYAYYARVAALVNPEATPE
                     PGVHPQAAVQAGARVAASASVGACAVVEEGAEIGDGSVIGANCFVGRGARIGRRTRLH
                     ANSTVYHDCVIGDRVIIHSGAVIGADGFGMAMDEGRWLKIPQIGRVVIGDDVEVGANT
                     TIDRGALDDTVIEEGVKLDNQIQIGHNCRIGAHTAIAGCVGIAGSTRIGSYCQLGGSA
                     MISGHLTIADHVIVSGG"
BASE COUNT         2851 a         4422 c         4790 g         2491 t
ORIGIN      
        1 cgaggaaacg tgtggggtgc cgatagacct gatctcgacc ggtcccgacc gcgaagagat
       61 gatcgtgctg cgccatcctt tcgcgcctgc aaggtaacgg gagtctgcga agcggattgg
      121 gggaggatgt caggctggtg ccgagaagag gactcgaacc tccacagtgt tgccaccgcc
      181 aggacctgaa cctggtgcgt ctaccaattc cgccatctcg gcacgggagg gcgcagaatg
      241 taattggaaa gcgctgcttt gtcaacgcag tttgcgcttt ccggaaccca aactttccat
      301 gaaaacaaac aagaaaacag aaatcgtcag ccgcaagaaa tcttcggcgc aggcgcgcgg
      361 caccgacgtg cgcagcaacg acccgcatct cgagcgcgag cagtcgcgtt acgagcatcc
      421 gcttccaagc cgcgaataca ttctccaggt tctgctcgag cagggcgtac cggttgccga
      481 ggacagactc gcgcaactgc tcggcgtcgc gccgcacgag atggacgcat tcgtgcggcg
      541 gctggcggcg atggagcgca gcggccagat catgcgcaac cgcaagaacg cgatctgcgt
      601 ggtggagaag ctcgacctgg tgaacgggcg agtgcagggc cacccggacg ggttcggctt
      661 cctgatccgc gacgatcgcg gcgaggacct gtttctcaat ccgggagaaa tgtccaaggt
      721 actgcacggc gacagggtgg tggcgcgcga gggcggagtg aatgcgcgcg gccggcgcga
      781 aggccagatc gtcgaagtac tcgaacgcgc caatcagaaa gtggtgggcc gcttcctcaa
      841 cgaacacggc atcggcgtgg tggcggcgga agaccggcgc atcagccagg acatcctcat
      901 ccccgccggc gccaccggcg gcgcgaaacc cggacaggtc gtggtggccg agatcatcga
      961 gcagcccaag ccccacgcgc ggcccattgg ccgcatcgtc gaggtgctcg gcaattacgc
     1021 cgatcccgga atggagatcg agatcgcgct gagaaagcac gacctgccgc acgtattttc
     1081 caaggaaatt gagaaggtgg ctgcgaagtt ccctgccgga gtgacggaga aagatctcaa
     1141 aggccgcgaa gacctgcgca acctgccgct ggtcacgatc gacggcgaga ccgccaagga
     1201 ttttgacgac gcggtctatg ccgagcgtaa cggcaagggc ttcaagctgt gggtggccat
     1261 tgcggacgtc agccactatg tgcagcacgg cgatccgatc gacctcgaag cgcgcctgcg
     1321 cggaaactcg gtgtatttcc cgcggcgtgt gatcccgatg ctgcccgagg tattgtcgaa
     1381 cggcctgtgt tcgctgatgc cgaaggtcga ccgcctgtgc atggtgtgcg aaatggacat
     1441 ctccgcacgc ggcgacattg gggcctatcg attctatcca gccgtgatgc attcgcgcgc
     1501 gcgactcacc tacacccagg tcgcgaaggt actcgaggat ccgcaaggcg agcccacgcg
     1561 cgagctgcgg caactggttc cgcaacttca gacgttgtac gccctgtacc agggcctcac
     1621 caaggcgcgc gcactgcgcg gcgcgatcga ctttgaaacc gtcgagaccc agatgttctt
     1681 cgacggccag ggcaagatcg agcgcatcgt ccccaccgag cgcaacgatg cccaccgcct
     1741 gatcgaggaa tgcatgctgg cggccaatgt ctccgcctcc gatttcctgt ccggccacga
     1801 tcatccggcg ctgtaccgcg tgcacgaagg cccgactccg gaaaagctgg cggcgctgcg
     1861 cgagtttcta cgcgagttcg ggctgcagct cactggcggc gaccaacctc aggccaagga
     1921 ctacgcgcgc ttgctggaaa aaatcaagac ccgcccggat gtgcaattgc tgcagacggt
     1981 gatgctgcgc tcgctgcgcc aggcgatcta cagcccggac aacgtcggcc atttcgggct
     2041 ggcctacgag tcctacaccc atttcacttc accgatccgc cgctatcccg accttgtcat
     2101 tcatcgttcc ataaaagcag tgctggcagg caaacgttac gacccaggcg actggcacga
     2161 gatcggcgtg cactgttccg agacggaacg ccgtgccgac gaggccaccc gcgacgtcga
     2221 ggcctggctc aagtgctatt acatgcagga tcgcatcagc gagcgcttca ccggcaccgt
     2281 cagcggcgtt accagcttcg gcgtgttcgt cgcgctcgac caggtgtacg tggagggtct
     2341 ggtgcacatt tccgaactgg ggtcggatta ttttcattac gatgccgcca agcatcaact
     2401 gctgggagag cgcaccggca aacgattccg gctcggcgac cgggtgtccg tcaagctggt
     2461 gcgggtggat ctcgagacca gcaagatcga cttcgtgctc gacgagccga agtcgaaggg
     2521 caaatgagcg gcaggcgggc gatacacggg ctgcacgctg tcaccgcggc gctcaggcgc
     2581 cggccggagg cggtgttcga agtgtttctg gaccccgggc ggaatgatcc gcgtgcccgc
     2641 agggtggcgg atctggccaa ggccggcagc gtgcgcgttc accatgccga cgcagcgcgc
     2701 ctcgatgggc tggcgaaggg ctcgcggcac caaggcgtgg tcgcctttgt cgaggaatcg
     2761 gccccggcgg gttcgctgga agaggtgctg gaaagcgcga ccgaaccgcc gttgctgctg
     2821 gtgctggacg gggtgcagga cccgcacaac ctgggtgcct gtctgcgggt cgcggacgcg
     2881 atgggggtgc atgcggtgat cgcacccaag gaccgggcgg ccggcctcag cgccaccgtg
     2941 cacaaagtcg cttcgggcgc ggcggaaagc gtgcccttta tcgtcgtcac caacctggca
     3001 cgcgcgctca gagacctgaa agataaggga atatgggtac tcggcgcggc cgaagccggc
     3061 gccaccacat tgttccaatc caggctttcc ggtgcgctgg cgctggtgct cggcgcggag
     3121 ggagaagggc tgcgacggct tacgcgcgag acctgcgacg aattgatcgc gatcccgatg
     3181 ctgggccagg tggaaagcct gaacgtgtcg gtggcggcgg gcatctgcct gtacgaagcc
     3241 cgtcggcagc gtcacgccgc cacaggcggg gcggtaaacc cttgagtgcg cataaaaatg
     3301 cttgaggcaa ccgggctcgc gctgatataa ttttttgtta gctccggtgg ttttgatcgg
     3361 agcaattcgc acgcttgcca gttcgcgccg ggtcatgccg gcgtgagcga taaaaaatcc
     3421 ggctctgccg gatcggaagg gtgcccgact atcgggttcc ggcgagcgga ggctcaaccc
     3481 ttacaggaga agttcatgtc ggtcaccatg cgtcagatgc tcgaagccgg agttcacttc
     3541 gggcaccaaa cccgttactg gaatcccaag atggccccgt ttatcttcgg ccatcgcagc
     3601 aagattcaca tcatcaacct cgagaagacc ctggccatgt accaggatgc ggtgaagttc
     3661 gtgcgccagc tggccgcaaa caagggcagc atcctgttcg tcggcaccaa gcgccaggcg
     3721 cgcgagatca tccgcgagga ggcgctgcgc gccggcacgc ccttcgtcga tcaccgctgg
     3781 ctgggcggca tgctcaccaa tttcaagacg gttaagcagt cgatcaagcg cctgcgcgat
     3841 atggagcaga tggtgcagga cggctcgatc gagaaactgt cgaagaaaga gcagctcaat
     3901 ttccagcgcg agatggtcaa gttggaccgc agcctcggcg gcatcaagga aatgaacgga
     3961 ttgccggacg cgctgttcgt ggtggacgtg ggttaccaga agggcgcggt caccgaagcc
     4021 aacaagctcg gcatcccggt ggtcggggtg gtggacacca accattcccc cgacggcgtg
     4081 gactacgtta ttccgggcaa cgacgatgcc agccgcgcgg tgcgcctgta cgtgcgcggc
     4141 atggccgacg cgatcctgga gggccgcagc cagatcgtcc agcagatcgt cagcgacgaa
     4201 ttcgtggagg tggacagttc ggaggaacag gcgccggcct gatccgggcc gtcgcagaaa
     4261 aaagggcgta cgcccttttt ttttaaccga agattcagaa accgcgcggc aggcgcgcaa
     4321 ccaggaacca ggagcaatcg agaatggcgg aaatcaccgc aaacatggtc aaggaactgc
     4381 gcagccgcac cgggctcggc atgatggaat gcaagaaggc gctggaagag gcgggtggcg
     4441 acatgaacaa ggctgaggac ctgctgcgta tcaggagcgg cgcaaaggcg agcaaggtcg
     4501 ccggccgggt cgctgccgaa ggcacgatcg gcattcactt gtcgggcgac cgcaagctgg
     4561 gtgcgctggt ggaagtgaac tgcgagaccg acttcgtcgc caaggaccag aatttcgccg
     4621 ggtttgcgaa agcggtggcg gaactcgccg cagccggcgg catcaccgat gtcgaggcgc
     4681 tgactcgggc acggcttgcc tcgggcgaga gcgtggaggc gacgcgccag gcgctggtga
     4741 tgaagctcgg cgagaacatc ggcgtgcgcc gggtcgcggt gcacaaggcc aagggcaggc
     4801 ttgcgcacta tttgcatggc accaggatcg gcgtgatggt cgacctggag ggcggcgacg
     4861 atacgctcgg caaggacctg gcgatgcacg tggcggcttc caagccgatt tccgtgtccc
     4921 gggaccaggt cccggcggaa ttgatcgcac gcgaacgcga gatcgccagg gcccgggcgc
     4981 tggaatccgg caagccggcc aacctcgtgg aaaaaatcgt cgagggcagc gtgcacaagt
     5041 atcttgccga ggtcacgttg ctgggccagc cattcgtcaa ggacgacaag accacggtgg
     5101 agaagctgct cgcggccaag ggcgcaaagg tgcatgcgtt cacgctgttc atcgtcggcg
     5161 agggcatcga gaaaaaggca accgatttcg ctgccgaagt gatggcgcag gctgcccagg
     5221 cgcgctgatc cggaccggcc gcggcgcgat gaagccagcc tacaagcgca tcctgctcaa
     5281 gctgtcgggc gaagccctga tgggcgacga tgcctacggg gtcaatcgcg agaccatcga
     5341 ccgcatcgtt tcggaggtca aggaagtggt ggggctcggg gtcgaggtcg gcgtggtcat
     5401 cggcggcggc aacatctttc gcggcatggc gccggcggcg gccggcatgg accgtgcgac
     5461 ggcggattac atgggaatgc tggccacggt gatgaacgcc ttagcgctgc aggacgcgat
     5521 gcgcaggcaa gggctcaaga gccgggtgca gtcggcgatc aatatcgagc aggtcgccga
     5581 accctatatc cgcggcaagg cgatgcgtta cctggaagaa ggccgggtag tcatcttcgc
     5641 cgccggcacc ggcaacccgt tcttcacgac cgatacggcg gcagcgctgc gcggcatgga
     5701 aatgaacgtg gacatcgtcg tgaaggccac caaggtggac ggcgtctata ccgacgatcc
     5761 caagacccat ccggacgcga tgcgttatca gcgcgtcagc ttcgacgagg cgatcgtcaa
     5821 gaagctcaag gtgatggacg ccaccgcatt gaccttgtgc cgcgaccaga agctgccgat
     5881 ctgcgtgttc agcattttca aggcgggcgc actcaaacgg gtggtgctcg gcgaagacga
     5941 aggcacgctg gtccacgcct gagaagctga aaagagggag cctatgacaa ttgcagacgt
     6001 gaagaaatcg gcggatcaga agatgcacaa gacgctggat acgctcaagg ccgatctcgg
     6061 caagatccgc accggccgcg cccacaccgg tatcctggat cacatccagg tcgactatta
     6121 cggtaatccc acgccgctca accaggtggc gaacgtgtcg ttgatagatg cccgcaccat
     6181 cggcgtcacg ccctgggaaa agaaaatggt cgcggtgatc gacaaggcca tccgcgaggc
     6241 cgatctgggg ttgaaccccg cgaccatggg ggacacggtg cgggtgccga tgccggcgct
     6301 gaccgaggaa cgccgccgcg acctgaccaa ggtcgtcaag ggcgaggccg agaatgcgcg
     6361 cgtggcggtg cgcaatatcc gccgcgacgc caacacggcg ctcaaggaat tgctgaagca
     6421 aaaagcggtg tcggaagacg acgagcgccg tgcccaggaa gacatccaga aactgaccga
     6481 ccgctacatc gccgagatcg acaaggcctt agcggccaaa gaagctgacc tgatggcggt
     6541 ctgacgcaaa cgtgttcacc agcgccaccc ttgccgttcc cgaagtccgc gccgtcccgc
     6601 gccatatcgc catcatcatg gatggcaacg ggcgctgggc caagaagcgc ttcctgccgc
     6661 gcgtggcagg tcatcgtcgc gggctcgaag cggtgcgcgc gaccacaaag gccagcatcg
     6721 aacgcggcgt cgagtatctg acgctgtttg cgttcagcag cgaaaactgg cgccgtccaa
     6781 aggacgaagt ttcgttcctg atggagttgt tcgtgatggc gctcgaacag gaggtgagca
     6841 agctccacgc caaccggatc cgtctcaggg tggtgggcga cctgtccagc ttcgagccgc
     6901 gtttgaccac tctgatcagc aacgcggagc agctgaccgc cggcaacgac cggctcacgc
     6961 tcaccattgc cgccaattac ggcggacgct gggacatcct gcaggcggtc aaccgcatgg
     7021 tgcgggaact gccgaatccg caggatgcgt accgcgaggc ccatcttgcc ccgcacctgt
     7081 cgatgagcta cgcgcccgag cccgatctgt tcatccgtac cggcggcgag cagcgcatca
     7141 gcaacttcct gttgtggcag ttggcctata ccgagttcta cttcaccgac gagttgtggc
     7201 cggatttcga cgctgaggcg ctggctcgcg ccattgactc ctaccgcagc cgggaacgcc
     7261 ggttcggccg caccagcgaa caactcggcg cggagtctgc gccggccgga acctcgcgtg
     7321 ggaccgttgg ggaatagcga tgatccggct gcggcgcaaa cctccgctgc agcgccccgc
     7381 cgcatgctca aacaacgttt gcttaccgtg gcggtggtct tgccgctgct gctcgcctgc
     7441 attttcctgc ttcccaatcc cggctgggcg ctgctgatga ccgttcccac gacgctcggc
     7501 gcgcttgaat gggcgaagct cgccgggtac ggacgcggcg ctcgcgcgct gttcatcgcc
     7561 gtggtgctcg ccagttgcct gatgctgatc gcggttgcag ccgcgccgcg attgctggcc
     7621 ctggcgatga atctcgcgac ggcgctgttc atgctggcgc tgctgttctg ggcgatcgcg
     7681 gtgccggtct ggctctacag caagtggcgg gtatcacagc cgctgctgct tgccgttgcc
     7741 ggctggatcg tactggtccc ggcctggctg gcggtggtcc ggctgcagca gtcggcgtgg
     7801 ctgctgctgg tcgtactcgg ggcggtgtgg gtcgccgaca ccgcagcgta tttcgccggc
     7861 caccgtttcg gacgcaacaa gctggcgccg cagatcagcc ccggcaagac ctgggaaggc
     7921 gtcatcggcg cctacatcgc cgtgctcggc tatgcgctgg cggcaagcct gatcctgcag
     7981 ccatcggcaa acggttacga tcgactcggc gtgttgctgt tcgcgtgcgt actgaccgcg
     8041 ttcggcatcg tcggcgacct gttcgagtcg tggatcaagc gccaggcagg cgccaaggac
     8101 agcggtcagt tgcttcccgg ccacggcggc gtgctcgacc gcatcgacag cctcaccgcg
     8161 gcgttgccgt tcgccgcgct gtacttcatc caggcggcgt ggtcagcgtg aagcgcctgg
     8221 ccatcctcgg ttccaccggc agcatcggcg ccagtacgct ggcggtggtg gaacgccatc
     8281 ccgaccgttt cgaagtgact gcattgtccg ccgatcgcca ggtggaccgg ctgctggcgc
     8341 aatgcctgaa gttccggccg aaatatgccg cgctggcgga cgcggcggcc gcggagcaac
     8401 tgcgcgtcga ggtgcgcgca gccgggctga aatgcgaggt gctggcgggc gaggcggcgc
     8461 tggcgcagat cgccgcggcg cccgaggtcg acgcagtaat ggcggcgatc gttggttctc
     8521 cgggtttggc ctcgactctg gcggcggcgc gtgctggcaa gcagctcttg ctcgccaaca
     8581 aggaggcgct ggtcatggcg ggcgcactgc tcatggacgc ggtacgggcc agcggtgcgg
     8641 tgctgctgcc gatcgacagc gagcacaacg cgattttcca ggcactgccg cgcgagttcg
     8701 acggcgactt cggccgcgcc ggggtcaaac gcctgtggct taccgcgtcg ggcgggccgt
     8761 ttctgcgggc ggcgcgggcg cagttggcgc aggtcacgcc cgagcaggcg tgcgcgcatc
     8821 cgaactgggt catgggccgc aagatctcgg tggactcggc cacgttgatg aacaagggac
     8881 tggaagtgat cgaggcgagt ctgctgttcc gggcgaaatc ggcgcagatc gaagtggtca
     8941 tacatccgca aagcattgtg cattcgctgg tcgagtacgt ggatggttcc gtgcttgcgc
     9001 aactgggcaa tccggacatg cgtaccccga ttgcccacgc actcgcttat ccggagcgca
     9061 tcgatccggg cgtcgaggcg ctcgacctgt ttgcggtcgc ctcgctcggc ttcgaacgcc
     9121 cggatctgga ccgctttccc agcctgaggc tggcgtaccg ggcgctgcag gcgggcggca
     9181 gcgcgccaat cctgctcaat gccgcgaacg aagtcgcagt ggcaagcttc ctcgaacgca
     9241 ggcttgcgtt tgaccgcatc cccgagctgg tggaagaagt gctggagcgc gtggcggcgg
     9301 ctccggtgca atctctggaa gacgtgctgg ccgccgattc acttgcgcgt cgcacggcga
     9361 tgcaaagact tgaaaccgtt acccattgaa tagccgatgt cgctgatttc gaccctgatc
     9421 gcatttgtcg tcgcgcttgg cgtgctgatc gtgtttcacg aattcggcca ctatctggtc
     9481 gcgcgcctgt gcggcgtcaa agtattgcga ttctcggtgg gattcggcat gccgctgtgg
     9541 aagcgaaagt tcggccgcga tggcaccgag tggacgatcg ccgcgtttcc actcggcggt
     9601 tacgtgaaaa tgctggacga gcgcgaaggc cccgtggcgg cggaggagcg ccatcgcgcc
     9661 ttcaacgtgc agccggtggc caagcgtttc gccgtcgttc tcgccggacc ggtcgccaat
     9721 tttctgctgg caatcctgct ctactggttg ctgttcgttc acggtctgcc tggaatccgt
     9781 ccggtcgtcg gcgccccact gccggacacg ccggcggctc atgccggatt tcacgccggg
     9841 gacctgatca ggcgcatcgg cgaggtgcag gtgagcactt ggcaggatgt ccgctgggtg
     9901 ttgctcgaga atgcactccg caaggaagcg gtgcgcgtcg aactgcagag cgaacgcggc
     9961 gacatcaatt tcggacggct ggatctgtcg cgaattggcc cggagcacat cgacggcgac
    10021 ttgctgcagt cggtcggtat cacccgctat caaccgccgc tcaagccggt catcggacgc
    10081 ctgatcgcgg gcggcgcggg cgagcgcgac ggactgaaac cgggcgacga agtcgtcgaa
    10141 gtggacggcc ggccgatcgc gcactgggat gaactggtgt cggcggtcag cacggccgcg
    10201 ggccgcgcgc tgacgatgcg tgtcaggcgc acggatacgg tgttccagct caccgtcact
    10261 ccggaagcca agaccgaagg cggcaagacc gtgggacgca tcggcgcggc cccgcacgtc
    10321 gacgaggctg aaatgaaaag actggtcacc gaggtgcgct acggcccggc agcagcggcc
    10381 gtgaaggcgc ttgagcgcac ctgggaagta tccctgttca cactcaagat gctcggcaag
    10441 atgatcgttg gcgagctgtc gctgaagaat ctgagcggcc ccatcaccat cgcggactat
    10501 gcgggccagt cggcgcagat gggctggctc gcctatctca gttttctggc cctgatcagc
    10561 atcagcctcg gcgtgctgaa ccttttgccg gtgccgttat tggatggtgg acacctcatg
    10621 tattatgtcg ccgaaatcat caaaggcagc ccggtatcgg accgggtgat ggaaataggc
    10681 cagcagctag gaatcgttct gctgttcagc ttgatggcgt ttgcaatcta taacgatata
    10741 aaccgcctgt tcggtggctg agagcggatg aagaaacgct tactgttact ggccttatcg
    10801 agcctcgtca gcctatccac cctcgctttc gaaccttttc aggtaaaaga catccgcgtc
    10861 cagggcatcc agcgcaccga ggcgggaacg gtgttcagct acctgccggt caaggtcggt
    10921 gacaccatcg atcaggaacg cgcgacctcc gcaatcaaag cgctctacgc caccggtttc
    10981 ttcaaggacg tccggctgga acaggatggc gacgtgctgg tggtcgtagt cgaagagcgg
    11041 ccggccatcg cgcagatcgc aatcgccggc tccaaggagt tcgacgccaa gcaactcaag
    11101 gacggactga aacagatcgg ccttgccgaa tcgcggattt tcgaccgcgc gctgctggaa
    11161 cgcgccgagc aggagttgaa gagccagtac atcagtcgcg gcaagtacgg cgtcaccatc
    11221 accaccactg tgaccccgct ggaacgcaac cgggtggcga tcaatttcga catttcggag
    11281 ggcgaggtgg cgaaaatccg ccagatcaac atcgtcggca acagcgtatt caaggacgac
    11341 aagctgcgcg acgaattcgt attgcgcacc cccggatggc tcacctggta cagcaagaac
    11401 gaccagtact cgaagcccaa gctgcagggc gacctcgagg ctctgcgtac cttctatctg
    11461 aatcagggtt atctcgagtt caccatcgat tcgacccagg tctcgatcac gcccgacaag
    11521 cgcgatatct tcatcacagt caacatcacg gaaggcaaga agttcaccgt cacggatgtg
    11581 aaactcgccg gcgaactgat cgcgccggaa gcggaactgc gcaaactgat caagctgaaa
    11641 aagggcgacg tgttttcgcg ggaacgtttg accgagacca ccaagctgat cggcgaccgg
    11701 ctcggcaacg agggctatgc ctttgccagc atcaatcccg tgccggaaat cgatcgcgac
    11761 aaaagtgaag tggcgttcac gctctatgtc gacccaggcc gccgcgtcta tgtgcggcgc
    11821 atcggcatca gcggcaacac caatacccgg gacgaggtca tccgccggga gatgcggcaa
    11881 ctggaaagcg gctggtactc caccgaaaaa ctgaaccgct cgaaacagcg tatcgacaag
    11941 ctcggtttct tttccgatgt gcaaatcgac acgccgggcg tacccggaac ctccgaccag
    12001 gtagatgtcg atgtcaaggt caacgagcgc gccaccggga atctcacttt cggagtcggc
    12061 tattccagcg cggaaaaagt gattctgtcg gcgggcgtgt cgcagagcaa catcttcggc
    12121 agcggaaacg cgctgtcctt tcaggtaaac accggcagca tcaaccgcac gtacgcgctt
    12181 tcctacacca acccgtatta caccgacgac ggcgtgagcc gcggcttcga catttacaaa
    12241 cgtgacgtca acgcgacatc gctggcgatc gccagctaca agacgtccac gattggcggg
    12301 ggcgtgcgct tcggcgtgcc ggtcaccgag tacgacacgg tcaattacgg acttggctac
    12361 gagcgcaccg agctcgaact gcagccgaac accccgctgc gctatgtgga tttcgtgaac
    12421 gagttcggcg cgcggaccga cacgattctg ggcagcgtgg gctggtcgcg cgacaagcgc
    12481 gacagcgtga tctataccac cagtggcaca ttccagcgca tcacggcaga cgtgggcttg
    12541 cccggcgccg acctgaccta ctaccggacc aatctgcagc atcagtggta tcatcctctg
    12601 actcgggact acacgctgca tttcaacgga caggtcggct acgcggacgg atacgacaaa
    12661 aagccgctgc cgttctataa agtgttctat ctcgggggcg taaactcggt gcgcggctac
    12721 gagaccgcat ccatcggccc gaaggactcg aacggggatt ctctgggcgg cagccaccag
    12781 ctgctggcca atctcgagtt cctgtttccg tttccaggtc tcgccaaaga ccggtcggtg
    12841 cggctgagcc ttttcgcgga cgcgggtgtt gtcggggaga agtatgactt cggcgagatg
    12901 cgctactcga ccggcctggc gttcaactgg ttctcgccgg tcggcccgct gaagttgagt
    12961 ttcggcaagg cgctgaaccc gcaaccggac gaccgcctgc aacgcataca gttctcgttc
    13021 ggcacgacat tttgaatcgg gccgcctgca gcaggcggcg cttgctccat acagaccaga
    13081 ggagaacgga agttgaacaa gatctcgctt gcgattgccg ccgtggcgct cttggcgagc
    13141 tcgaatgcgc ccgccgccgc tgagctgaaa atcggcttca tcaagaccga tcgggtgttc
    13201 agggaagcgg cgccggcggt gagggcgctg aaaaaaattg aaaaggaatt tgcgccccgc
    13261 gaccaggaaa tccagaagct cgccaagcag acgcgcgatc tgcagtcggt cctggaaaag
    13321 gaagcgatga ccatgagcga aaccgaccgt cgcgacaagg agcaggaact ggcgcgcatg
    13381 acccgggagc tgcagcgcat gcagcgcgag ttccgcgaag acctgaattt gcgcaggaac
    13441 gaggagatgg ccgccgtgct ggagcgtgcc cagaaggtga tacagcagat cgcggaaacg
    13501 gaaaaattcg acctgattct gcaggaggcg gtttacatga gtccgcggct cgatatcacc
    13561 gacaaggtca tcaaggcgct cgcagacaag taagacgctg cggtgtgcga cggccctttc
    13621 ccgggaacca tgcctgagtc cacgcctccg gcagcgccca tcacggcgac gctcgcatga
    13681 cgggggacgt ccgcaagtct ttcagcctgg cggagctcgc cgaccacttt ggcggaaccg
    13741 tggtcggtga tgccgccact cgcatccgcc aggtggcgcc gctggagacg gcggggccgg
    13801 aagagctgtc ttttcttgcc agccgcaaat atcagaaact tctagcttcc acgcgcgccc
    13861 gcgccgtaat cctgggcgag gaagcgcgcg atgccacggc cctgccgcgc atcgtctgca
    13921 agtatcccta cgcctattac gcgcgcgtcg cggcgctggt caatccagag gccacgcccg
    13981 aacccggggt ccatccgcag gcggcggtgc aggccggtgc gcgcgtggcg gcttccgcgt
    14041 cggtcggcgc ctgcgcggtg gttgaagagg gggcggagat cggtgacggc agcgtgatcg
    14101 gcgccaactg cttcgttggg cgcggcgcgc gcatcggcag gcgcacgcgc ctgcatgcca
    14161 actccaccgt gtatcacgat tgcgtgatcg gtgaccgggt catcatccat tccggggcgg
    14221 tgatcggcgc agatggattc ggcatggcga tggacgaagg gcgttggctg aagatcccgc
    14281 agatagggcg ggtggttatc ggcgacgatg tagaagtcgg cgccaacacg actatagacc
    14341 gtggcgcgct cgacgacacc gtgatcgagg aaggcgtgaa gctggacaac cagatccaga
    14401 tcggacacaa ctgccgcatc ggtgcgcaca ccgccattgc cggctgcgtc ggcatcgccg
    14461 gcagcacacg catcggcagt tactgccagc tcggcggcag tgcgatgata tcgggccatc
    14521 tgacgatcgc cgaccacgtg atcgtctccg gggg
//
LOCUS       DASQOP010000205         2509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_492053, whole genome shotgun sequence.
ACCESSION   DASQOP010000205 DASQOP010000000
VERSION     DASQOP010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492053"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="VE030_08380"
     CDS             complement(<1..862)
                     /locus_tag="VE030_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23466.1"
                     /translation="MLAADSQTSGDSFDDRFPAPQFNDRFSGASEPLVPRQFSDALRK
                     RMAQAEPAPYRVASLAPTVPYQRPAREEQTTLVSLKSSAFPYLGNNPRTDEPFLNVTN
                     GDRRGHRGLGGKVFWQDETYNDNRVLMHVPENFDVRKPGVIVVFFHGNGATLERDVRD
                     RQLVPQQISDAGVNAVLLAPQLAVDAADSSAGKFWQRGGLKRFVTESSEHLARLYGDP
                     GTAKAFANMPIVIVGYSGGFVPAAWSLEVGGLGNRVRGVFLLDAVYGELDKFASWIVD
                     NRSGFFVSAYT"
     gene            1349..>2509
                     /locus_tag="VE030_08385"
     CDS             1349..>2509
                     /locus_tag="VE030_08385"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZF23467.1"
                     /translation="MAASGAESAAWPGRLSGAGTTVSVMVATAIVVADMVGVGVFTSL
                     GFQVKDIPSGFSILLLWTVGGVVALCGVFSYSELGAMFPRSSGEYNFLTRAFHPAFGF
                     LAGWVSATVGFAAPVALAAMAFGEYGKAVLPGAPPLVLAIGVVWLVSIVQLGGVRHSG
                     TFQLISTILKVVLIVAFLIAGFVIGTPQPVSFTPSVSDFAHITSAPFAIGLVFVMYSF
                     SGWNAATYIIGEMRTPQQNLPRALLAGTLIVLVLYVALNAVFLHTTPIDQLSGQLDVA
                     RIAGSHIFGEIGGRVVGAMICIGLVSSISAMMWIGPRVMMTMGEDMPALRVFARKSSS
                     GAPVYAILFQLAVANLLLFTRSFEAVLDFIQFALLFCSFFTVLGVIKLRITQP"
BASE COUNT          428 a          775 c          792 g          514 t
ORIGIN      
        1 gcgtataggc gctgacaaaa aagccggacc ggttgtcgac aatccaggat gcgaacttgt
       61 cgagctcgcc atacaccgcg tcgagcagga acacgccgcg cacgcggttg ccgaggccgc
      121 ccacttcgag gctccaggcc gccggcacaa agccgccgct gtagccgacg atgacgatcg
      181 gcatgttcgc gaaagccttt gccgtgcccg gatcgccgta gaggcgggcg aggtgctcgg
      241 atgactcggt cacgaagcgc ttgagcccgc cccgctgcca gaacttgccg gcgctggaat
      301 cggcggcatc gaccgccagc tgaggcgcca gcaacacggc attgacgccg gcatccgaaa
      361 tctgttgcgg cacgagttgc cggtcgcgca cgtcgcgctc cagcgtcgcg ccgttgccgt
      421 ggaagaatac aacgatcacg cccggtttgc ggacatcgaa attctccggg acatgcatga
      481 gaacccggtt atcgttgtag gtctcgtcct gccaaaaaac cttgcccccg agaccgcgat
      541 ggccgcggcg gtcgccattc gtaacgttga ggaacggctc gtcggtgcgc ggattgttgc
      601 cgagataggg aaatgccgag gatttcaggc tgaccagtgt ggtctgttct tctctggcgg
      661 ggcgctggta cgggaccgtt ggcgctagcg aggcaaccct gtaaggggcg ggctccgcct
      721 gagccatgcg cttgcggagc gcatccgaga actgtcgcgg caccaagggc tcactggcgc
      781 ccgagaagcg atcattgaat tgcggcgccg ggaaccgatc gtcgaacgaa tctcctgacg
      841 tctgggagtc ggcggccagc atttcagcgt tcggcgcctt gccgcattga acgagtacga
      901 gcgagagcgg gacaagcgcg gacagaaggg cgatccagcg ccatcgatga gaaccggcgc
      961 catgctgcgc aacccgtgtc gacgcatgcc gggtcgcgcc cggtggcgtc acctggccca
     1021 catgatcaga cgtctggaat ttcggattcg ctccgaccat ccaccccttg ccccaatatc
     1081 gccaaaggcg atcggcgtat gtcagcctgc caacgttgag ggaacgttaa gcacccggaa
     1141 aaactcccca cttccagaac gcgaaacgga gcaccaaatc gtgtcgcgat tgtgggccgg
     1201 ccactttgct ttcgcaatgt ccgccttcaa tggtacaaaa ctaccatatt catgctcaca
     1261 tttagagctt tgttagcatt gcttgaattg ccactgccca gcctgcacga tgaaaagatc
     1321 gcaggcgtga cccaaagcgt ggaccggtat ggcggcatca ggagcggaaa gcgcggcgtg
     1381 gcccggccgg ctctccgggg ccgggaccac ggtctccgtc atggtggcga ccgccatcgt
     1441 cgtggccgac atggttggcg tcggggtctt caccagcctg ggctttcagg tcaaggacat
     1501 cccctcgggc ttttccattc tgctgttatg gacggtcggc ggcgttgtcg cgctctgcgg
     1561 ggttttttcc tacagcgaac tcggcgcgat gtttccgcgc tcgagcggcg aatacaattt
     1621 cctgacccgc gcctttcatc cagctttcgg atttctggcg ggctgggtgt cggcgacggt
     1681 cggcttcgcc gcaccggtgg cgctggcggc gatggctttc ggggaatacg gcaaagcggt
     1741 gctgccgggc gcgccgccgc ttgtgcttgc aatcggtgtg gtctggctgg tgtcgattgt
     1801 tcagcttggc ggcgtcaggc actccggcac ctttcaattg atctcgacca ttttgaaagt
     1861 cgtgctgatc gtcgcgttcc tgattgcggg atttgtcatc ggcacgccgc aaccggtctc
     1921 gttcacgccg tcggtttcgg acttcgccca catcaccagc gcgcctttcg ccatcgggct
     1981 ggtgttcgtg atgtattcgt tttcggggtg gaacgccgcg acctacatca tcggcgagat
     2041 gcgcacgccg cagcaaaacc tgccgcgcgc gctgctggcg gggacgctga tcgtcctcgt
     2101 gctctatgtc gcgctgaacg cggtgttcct gcacaccacg ccaatcgacc aactgtccgg
     2161 gcagcttgat gtcgcgcgga tcgccggcag ccacattttc ggtgagatcg gcggccgcgt
     2221 cgtcggtgcg atgatttgta tcgggcttgt gtcttccatc agcgcgatga tgtggatcgg
     2281 cccgcgcgtc atgatgacca tgggggagga catgccggcg ctgcgggtat tcgcccgcaa
     2341 atcgagctct ggcgcgccgg tctatgcgat cctgttccag ctcgcggtcg cgaacctgtt
     2401 gctgttcacc cgcagtttcg aggccgtact cgatttcatc cagttcgcgc tgttgttctg
     2461 ctcgttcttt accgtgctcg gcgtcatcaa gctgcgcatc acgcagccc
//
LOCUS       DASQOP010000206         4871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1899833, whole genome shotgun sequence.
ACCESSION   DASQOP010000206 DASQOP010000000
VERSION     DASQOP010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1899833"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..666)
                     /gene="tatB"
                     /locus_tag="VE030_08390"
     CDS             complement(196..666)
                     /gene="tatB"
                     /locus_tag="VE030_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HZF23468.1"
                     /translation="MFDIGFAEIFVIGVVALIVIGPERLPRVAKTVGHLFGRMQRYVS
                     EVKADINREIELDELRKLQSTMQDAARSIEQSVTSQVSFIESEVKQAGTEVQKQVESA
                     VAPVSGIQLMPTTTPASGSEPPAEEPAQNAPVPEEPEPQLELGLDKPAPDDKRA"
     gene            complement(696..920)
                     /gene="tatA"
                     /locus_tag="VE030_08395"
     CDS             complement(696..920)
                     /gene="tatA"
                     /locus_tag="VE030_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HZF23469.1"
                     /translation="MGSFSIWHWLIVLLVVLLIFGTKKLRNMGSDLGGAVRGFKEGMK
                     SADDEKTTARIDSSGAGQTIEGEVKDKTKS"
     gene            complement(945..1304)
                     /locus_tag="VE030_08400"
     CDS             complement(945..1304)
                     /locus_tag="VE030_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HZF23470.1"
                     /translation="MAAENCVFCKIVRGEIPSRKVYEDDEILGFHDINPVAPVHFMLI
                     PKEHVASLAHVEDRHQQVLGKMLLLAHRLASEQGSPDGFRAIVNTGRVGRQDVFHVHM
                     HVLGGRDPLPGMIQRSK"
     gene            complement(1361..1684)
                     /locus_tag="VE030_08405"
     CDS             complement(1361..1684)
                     /locus_tag="VE030_08405"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HZF23471.1"
                     /translation="MIDANILRRLAEVIDSRKHGDPGNSYIASLFAKGHDAVLKKVGE
                     EAAETLLAAKDGDKLHIVRETADLWFHCLVMLAWHGLGPDDVLAELRRREGISGIDEK
                     SSRPK"
     gene            complement(1681..2076)
                     /gene="hisI"
                     /locus_tag="VE030_08410"
     CDS             complement(1681..2076)
                     /gene="hisI"
                     /locus_tag="VE030_08410"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HZF23472.1"
                     /translation="MSDAWLDEVAWDEHGLVPAIAQEAGTGKVLMLAWMNREALAETV
                     RSGRAVYWSRSRGKLWRKGEESGHVQYVGEVRLDCDGDVVVLEVQQVGGIACHTGRSR
                     CFFRRLDNGAWVITEPVVKSPPEICESKP"
     gene            complement(2073..2843)
                     /gene="hisF"
                     /locus_tag="VE030_08415"
     CDS             complement(2073..2843)
                     /gene="hisF"
                     /locus_tag="VE030_08415"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HZF23473.1"
                     /translation="MGLAKRIIPCLDVTDGRVVKGVNFVKLRDAGDPVEIARRYDEQG
                     ADELTFLDITASSDNRGLILGIVEQVASQVFIPLTVGGGVREVNDVRRLLNAGADKVS
                     INTAAVQNPRLVEDAAGRFGSQCIVVAVDAKRVPGSGSLRWEVFTHGGRRSTGLDAVE
                     WGKKMQAAGAGEVLLTSMDRDGTKAGFDIELTCAFSEALDIPVIASGGVGGLQHLVDG
                     IVLGKADAVLAASVFHYGELTVQQAKQYMARHDIEVRL"
     gene            complement(3016..3786)
                     /gene="hisA"
                     /locus_tag="VE030_08420"
     CDS             complement(3016..3786)
                     /gene="hisA"
                     /locus_tag="VE030_08420"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HZF23474.1"
                     /translation="MLIIPAIDLKDGHCVRLKQGSMADATVFSEDPGAMARHWLKLGA
                     RRLHLVDLNGAFAGKPRNEEAVKAVVKAVGEDIPVQLGGGIRDLDTIERYLDDGIAYI
                     IIGTAAVKTPGFLHDACYAFPGHIMVGLDARDGKVAVDGWSKVTGHDVIDLAKKFQDY
                     GVEAVIHTDIGRDGMMSGVNIKATVQLARELSVPVIASGGITNIKDVKALCEVESEGI
                     MGAITGRAIYEGTLDFTEAQKVADRMAEKTAKAKEGEA"
     gene            complement(3880..4566)
                     /gene="hisH"
                     /locus_tag="VE030_08425"
     CDS             complement(3880..4566)
                     /gene="hisH"
                     /locus_tag="VE030_08425"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HZF23475.1"
                     /translation="MTDIAVVDYGMGNLRSVAKALEHVAPRKVVSVTSDPVVLRSASR
                     VVFPGQGAMPDCMREMDARGLRAPLLEAASSKPFLGLCIGLQMLFESSEEGGVEGLGI
                     LPGKVGRFPAERMVGERGEKLKVPHMGWNQVYQTMSHPMWTGIPDGSRFYFVHSYYVN
                     EREQSLVAGTARYPFSFTCAVARNNIFAVQFHPEKSQSAGLRLLSNFVSWDGTSRASG
                     QKRAIPTASA"
     gene            complement(4651..>4871)
                     /locus_tag="VE030_08430"
     CDS             complement(4651..>4871)
                     /locus_tag="VE030_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HZF23476.1"
                     /translation="GLVGEFDIDLVHEFFQGFANHALVTLHIDNFKGRNAHHQAETIF
                     KAFGRALRMASEMDPRAAGALPSTKGSL"
BASE COUNT          938 a         1588 c         1374 g          971 t
ORIGIN      
        1 agcaagggtt ttgcaagcat ggagtagata ttgcgcgccc acggaaacag gcaaatgaac
       61 accagcacaa gcgcgatcac gcaccgtaac agccggtccc tgagttcgac caggtgcgac
      121 atgaaggaat cgggctcgat cattatggac cgctggtttg tgttactgaa gcgaggttcg
      181 acgcgctcct cggaatcagg cgcgcttgtc gtccggggcc ggcttgtcca atcccagttc
      241 gagctgcggc tccggctctt cgggcacagg tgcgttctgc gccggttctt cggcaggagg
      301 ttcgctcccg ctcgccggcg tggtggtcgg cattaactgt attccggaaa ccggggccac
      361 ggcggattcc acctgcttct ggacttcggt cccggcctgc ttgacttcgg attcgatgaa
      421 gctgacctgc gaggtgaccg actgttcgat ggagcgcgcc gcatcctgca tcgtggactg
      481 cagcttgcgc agctcatcga gttcgatttc acggttgatg tccgccttga cctcggaaac
      541 gtagcgttgc atgcggccaa acagatggcc gacggttttc gctacacgcg gcagacgctc
      601 ggggccgatt acgatcagcg caacaacccc gatgacgaag atctcggcaa aaccgatatc
      661 gaacatgacg ctcgccaaaa agcggccgta tcccgctagc tcttggtctt gtctttgact
      721 tcgccttcga tagtctgacc ggcgccgcta ctgtcgatcc tggccgtcgt tttctcgtcg
      781 tccgcgctct tcatcccttc cttgaagccg cgcactgcac cgccaaggtc ggaacccatg
      841 ttgcgaagct tcttggtgcc gaaaatcagc agcaccacca gaagaacgat tagccaatgc
      901 caaatgctga aggaacccat cttttcactc ccgatacgaa atgtttactt tgaccgctga
      961 atcattccag gcagcggatc cctgccgccg agcacatgca tgtgtacatg aaacacgtcc
     1021 tgccgtccca cccgcccggt attgacgatg gcgcggaacc cgtccggcga tccctgctcg
     1081 cttgccagcc ggtgtgcaag cagcagcatc ttccccagca cctgctggtg ccgatcctcg
     1141 acgtgtgcga gcgatgccac gtgctccttg gggatcagca tgaagtgcac cggcgccacc
     1201 gggttgatgt cgtgaaagcc cagtatctcg tcgtcctcgt acactttgcg gctggggatc
     1261 tcgcccctga cgatcttgca gaaaacgcaa ttctcggcgg ccatttttct ggttgattgc
     1321 cccgcaggtt cttcgataac gatccagtcc gctggccgga ttacttcggt cgggatgact
     1381 tctcgtctat cccggaaatg ccctcgcggc gtctcagttc ggcaagcacg tcgtcgggcc
     1441 cgagaccgtg ccatgcaagc atgaccaggc agtgaaacca caggtccgcg gtttctcgaa
     1501 cgatatggag tttatcaccg tccttcgcgg cgagcagcgt ctcggccgcc tcctcgccga
     1561 ccttcttgag cacggcatcg tggccttttg caaacaggct cgcgatgtag gagttgcccg
     1621 gatcgccgtg cttgcggctg tcgatgacct ccgcaagccg gcgcaggatg ttggcgtcta
     1681 tcatggcttc gactcgcaga tttccggcgg actcttgacc accggttcgg tgatgaccca
     1741 tgcgccattg tcgagcctgc gaaagaaaca gcgactgcgg ccggtgtggc aggcaattcc
     1801 gccgacctgt tgcacctcga gcaccacgac atcgccatcg cagtcgagcc tgacttcccc
     1861 gacatactgg acgtggccgg attcctcgcc cttgcgccag agtttgcctc gcgagcgcga
     1921 ccagtatacc gcccgccccg aacgcacggt ctcggcgagc gcttcccggt tcatccacgc
     1981 gagcatcaac accttcccgg tgcccgcctc ctgcgcaatc gctggaacca ggccatgctc
     2041 gtcccaggcc acctcgtcga gccaggcgtc gctcacagcc gtacctcgat gtcgtgtcgc
     2101 gccatgtact gcttggcttg ctgcaccgtc aactcaccgt aatggaacac gctggcggcg
     2161 agcacggcat cggccttacc aagaacgatt ccatccacca ggtgctgcag gccgccaacg
     2221 cccccgctcg cgatcacggg aatatcgagg gcttcggaaa atgcgcaagt aagctcgata
     2281 tcgaaccccg ctttggtgcc gtcccggtcc atgctggtaa gcagaacctc gcctgcgcct
     2341 gcggcctgca tttttttccc ccactcgact gcgtccaacc ctgtggagcg ccgtccgccg
     2401 tgcgtaaaca cttcccagcg caagctacca gagcccggaa cacgcttggc gtccaccgcg
     2461 accacgatgc attgtgaacc aaagcgtccc gctgcatctt ccaccagtcg cggattctgc
     2521 actgcagcgg tattgatact gaccttgtcg gcgccggcgt tgagcagccg gcgcacatca
     2581 ttgacctcgc gcacgccgcc gccgaccgtg agcgggatga acacctgcga cgcaacctgc
     2641 tcgacgatgc cgagaatgag cccgcgattg tccgagctcg cggtgatatc caggaaagtc
     2701 aattcatcgg caccctgctc gtcgtacctg cgggcaatct ccaccgggtc gccggcatca
     2761 cgcagcttta caaaattgac accctttacc acgcgcccat cggtgacatc gaggcagggg
     2821 atgatgcgtt tggcaaggcc catatgcgtc gtacgtgata tctcaggcaa gaggaaaagg
     2881 gtcgacgaag cacaccgcaa aagcgaccgg gtcgcggcgg gtcgcagtga atcgaatcaa
     2941 ggaagatcgc gtggaccgag cccgctgccg gaccgggcgc cgcgtatccg caactcgggc
     3001 ctcaccactt acacctcatg cctcgccctc cttcgccttg gccgtcttct ccgccatcct
     3061 gtcagcgact ttctgtgcct cggtaaaatc cagcgtaccc tcgtaaattg cacggccggt
     3121 aattgcgccc ataatccctt ccgattcgac ttcgcacagc gccttaacgt ccttgatgtt
     3181 ggtgatcccg ccgctggcga tcaccggaac cgacaattcg cgcgcgagct ggaccgtcgc
     3241 cttgatgttg accccgctca tcatgccatc gcggccgata tcggtatgga tgaccgcctc
     3301 aacgccataa tcctggaatt tcttggccag gtcgatcacg tcgtggccgg tcaccttcga
     3361 ccagccgtcc accgcaactt tgccgtctct ggcatcgagc ccgaccatga tgtgcccggg
     3421 aaaggcgtaa caggcatcgt gcagaaatcc cggggtcttc accgcggccg tgccgatgat
     3481 gatgtaagca atgccgtcgt caaggtagcg ctcgatggta tcgaggtcgc ggatgccgcc
     3541 gcccagttgc acgggaatat cctcaccaac ggctttaacg acggccttca cggcctcttc
     3601 gttcctgggc ttgccggcaa aggcgccgtt caggtccacg aggtgcaggc gccgggctcc
     3661 cagtttcagc caatggcgcg ccatggcacc gggatcttcg gaaaagaccg tggcgtcagc
     3721 catcgagcct tgcttcagac ggacacagtg tccgtccttg aggtcaatcg ccggaatgat
     3781 cagcatgggt tggatatcgc tcgctgcagg cgtcgcaagt ggcgcgcgcc agttgaggcc
     3841 gttgcgacag gcaaggggat ccttacgggt tacaaggggt taggcgctcg cggtgggaat
     3901 cgcccgtttt tggccggacg cgcggctggt tccgtcccag gagacgaaat tcgacaacag
     3961 gcgcaggccg gcagactggc ttttttcagg gtggaactgc acggcgaaaa tgttattcct
     4021 cgcaacggcg caggtaaagc tgaaaggata acgggcggta cccgccacca aagactgttc
     4081 ccgctcattc acgtagtagc tgtgcacaaa atagaagcgg ctgccatcag gaatacccgt
     4141 ccacatcggg tgggacatgg tctgatacac ctggttccag cccatgtgcg gcaccttgag
     4201 cttctcgccg cgctcgccta ccatgcgttc ggcggggaag cgaccgactt tcccaggcag
     4261 tatgcccaaa ccctcgacgc caccctcttc gctcgactca aacaacatct gcaagccgat
     4321 gcacagaccc agaaacggct tgctactcgc cgcctccagt aacggcgcgc gcaatccgcg
     4381 cgcgtccatc tcacgcatgc agtccggcat ggcgccctgt cccggaaaca ccaccctgga
     4441 tgctgagcgc agcactaccg gatcactggt cacgctaaca accttgcggg gggcgacatg
     4501 ctccaaggcc ttcgccaccg aacgcaggtt gcccattccg taatcaacga cagctatatc
     4561 ggtcatacac gcgcgcttgg tgattcgtgg gtcgtcttca acattacttg ccgaagcata
     4621 tgcgggcgga aacccgttcc gcaaaaacgc tcagaggctc cccttggtcg agggcaacgc
     4681 gccggccgcc cgcggatcca tctcggaagc catgcgcagc gcgcgcccga aagccttgaa
     4741 gatggtctcc gcctggtggt gcgcgttcct gcctttgaag ttgtcgatgt gcaaagtcac
     4801 cagcgcgtgg ttagcgaagc cctggaagaa ttcatggacc aggtcgatgt cgaattcgcc
     4861 gaccagcccg c
//
LOCUS       DASQOP010000207         7848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1299522, whole genome shotgun sequence.
ACCESSION   DASQOP010000207 DASQOP010000000
VERSION     DASQOP010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7848
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1299522"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..751
                     /locus_tag="VE030_08435"
     CDS             23..751
                     /locus_tag="VE030_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HZF23477.1"
                     /translation="MEVNAMSRSLQGTGASRRLRRTGKVPGIVYGGEQPAQNIELDHN
                     SLFHQLKQETFHASILSMDLDGKKDRVLLRDVQMHPWKQQVLHVDFQRVSANKKIHME
                     VPLHFINADIAPGVKTGGGIVSHVMNEIDITCLPDDLPEFVEVDLQHLELGHSVHLAD
                     VKLPPGVDSTQLVRGDNAVVATVQIPRALVEPEPEVAPVAEAAAVEGAPAADAAAAAP
                     GTAPAAAGAKKEPEKKEGDKGAKK"
     gene            833..1408
                     /gene="pth"
                     /locus_tag="VE030_08440"
     CDS             833..1408
                     /gene="pth"
                     /locus_tag="VE030_08440"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HZF23478.1"
                     /translation="MKLVVGLGNPGREHDGTRHNAGFWWVDRLAAHSHVQLRQEARFH
                     GQAAKLVEGGRECWLLKPNTFMNLSGRAVQALTQFYKIAPAEILVVHDELDLPPGQVK
                     LKNGGGVAGHNGLKDIAAHLGPEFWRLRIGIGHPGDRGQVTNYVLNAPRKEELPLIRG
                     AIERSLELWPLLLEGKTEAAMLKLHTKRDEQ"
     gene            1469..2563
                     /gene="ychF"
                     /locus_tag="VE030_08445"
     CDS             1469..2563
                     /gene="ychF"
                     /locus_tag="VE030_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HZF23479.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTRAGIAAENYPFCTIEPNVGIV
                     EVPDPRLAQIAAIAKPQKVVPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAVVH
                     VVRCFTDDNVVHVSGKIDPLSDIETIDTELALADLATADKALARYRKPAQSGDKDAKL
                     LVAVLEKVQSQLNQAKPVRSLALSKEELTNIKPFCLITAKPALYAANVAEHGFGDDNA
                     LLQLVRQHAAREGAPVVAFCARMEAEIADLSDEDKQVFLADMGLKEPGLSRLIRAGYD
                     LLGLQTFFTAGEKEVRAWTIHKGDTAPQAAGVIHTDFEKGFIRAEVIAFGDYIAYKGE
                     HGAKEAGKMRLEGKEYVVKDGDVMHFRFNV"
     gene            complement(2570..3481)
                     /locus_tag="VE030_08450"
     CDS             complement(2570..3481)
                     /locus_tag="VE030_08450"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZF23480.1"
                     /translation="MPYDHSRPSLAGTACVLLAAMAFSAKAVIVKLAYAHAVDAATLL
                     ALRMLFSAPFFALMALWSGRGAAAVSLTRKDWLSVALLGFIGYYLASYLDFLGLQYIT
                     AGLERLILFLYPTVVVLLSAWLLKKPVRRHHIAALVLSYGGIALVFAENLEFGGHSFE
                     VVLGGALVFASGVIYSFYLIGTGEVIGKLGAARFTSYAMLVACMVAILQFLVAHDLSA
                     LALPRQVYWLSLLMAVVSTVIPAWLMSEGIRRTGANHAAMVGSVGPVVTIYLGYRILN
                     EPISAIQLVGAALVLAGVTLVSVKKSS"
     gene            3719..4444
                     /locus_tag="VE030_08455"
     CDS             3719..4444
                     /locus_tag="VE030_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZF23481.1"
                     /translation="MAGHSKWANIKHKKAAADAKRGKIFTRLIKEITVAARLGGSDSS
                     TNPRLRLAMDKAYASNMPKDNVERAIKRGSGELEGVSYEEVRYEGYGIGGAAVMVDCM
                     TDNRIRTVADVRHAFIKHGGNLGTDGSVAFLFKHCGQLLFPPGTSEEKVMNAALDAGA
                     EDVIANDDGSIEVITAANDFMGVKEKLEKRGLRAEVAEVTMKPTVENVLAGDDAAKMQ
                     RLLDALEAIDDVQDIHTTAVMEG"
     gene            4726..5247
                     /gene="ruvC"
                     /locus_tag="VE030_08460"
     CDS             4726..5247
                     /gene="ruvC"
                     /locus_tag="VE030_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HZF23482.1"
                     /translation="MRILGIDPGLRVTGFGVLDKVGQKLTYVTSGCIRTSHDGELPER
                     IKAILEGLCQLIGEIKPQQVAVEKVFVNVNPQSTLLLGQARGAAICAAVISSLPISEY
                     TALQVKQAVVGNGHARKEQVQEMVRRLLKLSGDPSADAADALACAICHAHGGQGMGQI
                     ATRGYRMKRGRLV"
     gene            5244..5822
                     /gene="ruvA"
                     /locus_tag="VE030_08465"
     CDS             5244..5822
                     /gene="ruvA"
                     /locus_tag="VE030_08465"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HZF23483.1"
                     /translation="MIGRITGQLIEKRPPTVVVDVHGIAYEVDVPMSTFFVLPATGST
                     VALHTHLVVREDAHLLFGFSTEEERQVFRQLLKISGVGARTALAVLSGLSVSELYRAV
                     STQDGARLTKIPGIGRKTAERLLLELRDRLAGGVAPSASGSAGSVRGDVLNALSALGY
                     SDKEAGAALAKVSPEAAVPEAIRQALKLLSKA"
     gene            6031..6813
                     /locus_tag="VE030_08470"
     CDS             6031..6813
                     /locus_tag="VE030_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23484.1"
                     /translation="MIVDTRQVAAALLALAIALPVAANPETASGKNDQIAEIIDLHDL
                     KTSVSIGNYYLKQESLLAIRALLARLGREQNLGPEWNPAKPLWQRAEGKLLGAVTARI
                     AEDWASLDWLRPQWHDLDNREFSSEELDVLLSHLRSDVGRKQVRIMDHTVSTHVMMAL
                     SFSGKLRDVAGVEADRTRMQDLWNAEDAEMRFSIQDAANVEGQRFALSPLGKKYFVTA
                     ILKLTGIVNRRIDEVAGMLPKQVDAYADQVRPLLEEFKSGRG"
     gene            6848..>7848
                     /gene="ruvB"
                     /locus_tag="VE030_08475"
     CDS             6848..>7848
                     /gene="ruvB"
                     /locus_tag="VE030_08475"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HZF23485.1"
                     /translation="MIETDRLIQPVPISTQEEALERALRPKQLDEYIGQEKIRGQLQI
                     FIQAARNRSEALDHVLLFGPPGLGKTTLAHIIAREMGVNLRQTSGPVLERPGDLAALL
                     TNLEPNDVLFIDEIHRLSPVVEEILYPALEDFQLDIMIGEGPAARSVKLELPPFTLVG
                     ATTRAGMLTNPLRDRFGIVSRLEFYSPAELTQIVERSARLLVVDIAAGGALEIATRSR
                     GTPRIANRLLRRVRDFAEVKAQGKVTKDVADAALKMLDVDHIGLDVMDRKLLLAVLEK
                     FGGGPVGIENLAAAIGEERDTIEDVLEPFLIQQGYLQRTPRGRMATVLTYRHFGAALP
                     "
BASE COUNT         1639 a         2321 c         2572 g         1316 t
ORIGIN      
        1 ttaggagagc aacatggcaa agatcgaagt caatgcgatg tcccgctcgc tgcagggaac
       61 gggtgcgagc cgccgtctgc gcagaacggg caaggtgccg ggaatcgtct atggaggcga
      121 gcaaccggcg cagaacatcg agctggacca caattcgctg ttccatcagc tcaagcagga
      181 gaccttccac gcttcgattc tgagcatgga cctggacggc aagaaggacc gcgtgctgct
      241 gcgcgacgtg cagatgcatc cgtggaagca acaggtgttg catgtcgatt tccagcgggt
      301 atcggccaac aagaagatcc acatggaggt gccgttgcac ttcatcaacg ccgacatcgc
      361 cccgggggtg aagaccggag gcgggatcgt cagccatgtg atgaacgaaa tcgacatcac
      421 ctgcctgccg gacgatctgc ccgaattcgt tgaagtcgac ttgcagcatc tcgaactcgg
      481 ccattcggtg catcttgccg atgtgaagct gcccccgggt gtggattcga cgcaactggt
      541 gcgcggcgac aatgctgtag tcgcaaccgt gcagataccg cgtgcgctgg tcgagccgga
      601 gcccgaagtc gctcctgtcg ccgaagccgc cgcagtggaa ggtgcacccg ccgccgacgc
      661 cgcagcagcg gctccgggca cggcaccggc cgctgcaggc gccaagaagg agcccgaaaa
      721 gaaggaaggc gacaagggcg ccaagaagta attttcctct tcctggctgt acggcccgcc
      781 cgtccgtgcg tccggcgggc cgtttcattt tccggaatcg ccgacaaaca acatgaagct
      841 cgtcgtagga ctgggaaatc cgggacgcga acatgacggc acccgccaca acgccggttt
      901 ctggtgggtc gaccgtctgg ccgcgcacag ccacgtccag ttgcggcagg aagcgcgttt
      961 tcacggccag gccgccaagc tggtcgaggg cggccgcgag tgctggttgc tcaagcccaa
     1021 taccttcatg aacctcagcg gccgcgcggt gcaggcgctc acccagttct acaagatcgc
     1081 gccggcggag atcctggtag tccacgacga acttgacctg ccgccgggcc aggtcaagct
     1141 caagaacggt ggcggcgtgg ccgggcacaa cggtttgaag gatatcgccg cgcatctggg
     1201 gccggaattc tggcggttgc gcatcggtat cggccatccc ggcgatcgcg gacaggtgac
     1261 gaactatgta ttgaacgcgc cgcgcaagga agagctgccg ctgattcgtg gggcgatcga
     1321 gcgcagtctg gagctgtggc cgctgctgct ggaaggcaag accgaagcag cgatgctgaa
     1381 actgcacacg aagcgtgatg agcaatgagc aatgggaaat gagcaaacag ccacagttcc
     1441 catcgcccat ctctcataac ccgtcgccat gtcgctaaag tgcggaatcg tcggactgcc
     1501 caacgtcggc aagtccacgc tgttcaacgc gctcaccagg gccgggatcg cggcggagaa
     1561 ctatccgttc tgcaccatcg agccgaacgt gggcatcgtc gaagtgccgg atccccggct
     1621 ggcgcagatc gctgctatcg ccaagccgca gaaagtggtt cccgcgatcg tggagttcgt
     1681 ggacatcgcc ggtctggtgg ccggtgcctc caaaggcgaa ggtctgggca accagtttct
     1741 tgccaacatc cgtgagaccg atgcggtggt gcatgtggtg cgttgcttta cggatgacaa
     1801 cgtggtgcat gtctccggaa aaatagatcc gctctccgac atcgaaacca tagataccga
     1861 actggcgctg gcggacctgg ccacggcgga caaggcgctc gcccgatatc gcaagccggc
     1921 gcagtccggc gacaaggatg cgaagttgct ggttgccgtg ctggagaagg tgcaatcgca
     1981 actgaaccag gccaagccgg tgcgctcgct cgctctttcg aaggaggagc tgaccaacat
     2041 caagccgttc tgcctgatta cggccaagcc ggcgctgtat gccgccaacg tcgcggagca
     2101 cggattcggc gacgacaatg cgctacttca actggtcagg cagcacgccg cccgggaagg
     2161 cgcgccggtc gtggcgttct gtgcgcgcat ggaagcggaa atcgccgatc tttccgacga
     2221 agacaagcag gtgttcctcg ccgacatggg cttgaaggaa ccgggcctga gccggttgat
     2281 ccgcgccggc tacgacctgc tggggttgca gaccttcttc accgccgggg aaaaggaagt
     2341 gcgggcgtgg acgatccaca agggcgacac cgcgccgcag gccgccgggg ttattcacac
     2401 cgatttcgag aaaggcttca tccgcgcgga ggtcatcgcc ttcggcgact acatcgccta
     2461 caaaggggag catggcgcaa aggaagccgg aaagatgcgc ctcgagggca aggagtacgt
     2521 cgtcaaggac ggcgacgtca tgcacttccg atttaatgtc taggaaggtc taggaacttt
     2581 tctttacgct gaccagcgtc acgccggcaa gcaccagcgc cgcgccgacc agttgaatcg
     2641 cgctgatagg ctcattcagt atccggtaac cgagatagat cgtgactacc ggtccgaccg
     2701 agccgaccat cgcggcatgg ttggcgccag tgcggcggat gccctccgac atcagccatg
     2761 ccggaatgac tgtggaaacg accgccatca aaagcgacag ccagtagacc tggcgcggca
     2821 gcgccaacgc cgacaggtca tgggcgacca ggaactgcaa gatggccacc atgcaggcca
     2881 ccagcatggc gtaggaggtg aagcgcgcgg cgccaagctt gccgatcact tccccggtgc
     2941 cgatcaggta gaacgagtaa atcacaccgc tggcaaacac cagcgcgccg ccgagcacca
     3001 cttcgaacga gtgaccgcca aattccaggt tttctgcaaa caccagtgcg atgccgccgt
     3061 aggacagtac cagcgcggca atgtggtggc ggcgaacggg cttcttcagc aaccacgctg
     3121 acagcagcac caccacggtc gggtagagga acaggatcaa ccgctcgagg ccggcggtga
     3181 tgtactggag gccgaggaaa tccagataac tggcgaggta gtaaccgatg aatccgagca
     3241 gcgccaccga tagccaatcc ttgcgggtca gagataccgc ggccgcgccg cggcccgacc
     3301 acagcgccat gagcgcgaag aagggcgccg agaaaagcat gcgcagcgcc agcagggtgg
     3361 cggcatcgac cgcatgggca taggccagct tgacgatgac cgctttcgcg gagaaggcca
     3421 tcgccgcaag caggacacaa gccgtgccgg cgagcgaagg ccgggagtgg tcgtacggca
     3481 tgggcggccg cgttcagcgc cggctgccgc cgccgagtat cgagccgagc acgccgcgga
     3541 cgatgcggcg gccgacttcc gagccgatcg gcagagactg ttgcatcggt ttctccggga
     3601 ccggagagga tcgggtggta tgataaaatt ttaattatat cagccgtata cggtaccact
     3661 tcaggttggc gcggcgattt ggatccggcg ccgttgcggc gccttcgcag aggaagtcat
     3721 ggcaggccac agcaagtggg caaacatcaa gcacaagaaa gccgctgcgg atgccaagcg
     3781 cggcaagatt ttcacccgcc ttatcaagga aatcaccgtc gccgcccgtc tgggcggctc
     3841 cgactcgagc accaatccgc ggctgcgcct ggcgatggac aaggcgtatg ccagcaacat
     3901 gccgaaagac aatgtggagc gcgcgatcaa gcgcggcagc ggtgaactcg agggcgtgag
     3961 ttacgaggaa gtgcgctacg agggttacgg aattggcggc gcggcggtca tggtggactg
     4021 catgaccgac aatcgcatcc gcacggtcgc ggacgtgcgc catgcgttca tcaagcacgg
     4081 cggcaatctc ggcaccgatg gttcggtcgc cttcctgttc aagcattgcg gacaattgct
     4141 gttccctccc ggcaccagcg aggaaaaagt catgaatgcg gcgctggatg cgggcgccga
     4201 agacgtgatt gccaacgacg acggcagcat cgaggttatc accgcggcca acgatttcat
     4261 gggggtgaag gaaaagctgg agaagcgcgg cctgagggcg gaagtcgcgg aggtgaccat
     4321 gaagccaaca gtggagaatg tgctggcggg agacgacgcg gcgaaaatgc aaaggctcct
     4381 cgatgccttg gaggcaatcg acgatgtgca ggacatccac accactgcgg tgatggaagg
     4441 gtgaggcgag cgacaaaagg cgtgaggcga gaggcgtgat aagagacacg cggcctcacg
     4501 gggctccgca gagcggaaat ctccgccatc cgctcgacct gctgaaggaa cgctgaatga
     4561 ggttcaccgc tgcacgcctc tcgcctcacc gaccatgagg attctgggaa tcgatccggg
     4621 gttaagggtg acggggttta gctccggcgc aacgtgcggc tgtcgccgaa cattaacctc
     4681 ctctgaaact ggtgacggga aagtcctcct cgattgggac aacttgtgag aatcttgggt
     4741 attgatccgg gattgcgggt caccggtttt ggggttctgg acaaagttgg ccagaagctc
     4801 acttacgtta ccagcggctg tatccgcacc tcgcacgatg gcgaactgcc ggagcgcatc
     4861 aaggccattc tcgaggggtt gtgccagttg atcggcgaga tcaagccgca gcaagtcgcg
     4921 gtggaaaaag tattcgtcaa cgtgaatccg caatccacct tgctgctcgg ccaggcgcgc
     4981 ggcgccgcaa tttgtgccgc ggtgatcagc agcctgccga tatcggagta caccgcgtta
     5041 caggtcaagc aagccgtggt cggcaacggc cacgcgcgca aggaacaggt gcaggagatg
     5101 gtgcgccggc tgctcaagct gtcgggtgac cccagcgccg acgccgccga cgcactggcc
     5161 tgcgcgatct gtcacgcgca cggcggccag ggaatggggc agatcgcgac gcgggggtac
     5221 cgcatgaaac gcgggaggct ggtgtgatcg gccgtatcac cgggcagctg atcgaaaaac
     5281 gtccgcccac cgtggtggtc gacgtgcacg gcatcgccta cgaagtggac gtgccgatga
     5341 gcactttctt tgtgctgccg gcgaccggct cgaccgtggc gctgcacacc cacctggtgg
     5401 tgcgcgaaga cgcgcacctg ctgttcggct ttagcaccga ggaggagcgg caggtgtttc
     5461 ggcaattgct caagatcagc ggtgttggcg cccgcacggc gctggcggta ttgtctggtc
     5521 tgtcggtatc ggagctatac agggcagtgt ccacacagga tggcgcgcgt cttaccaaga
     5581 ttcccggcat tggcaggaaa accgcggagc gcttgttgct cgaacttcgc gataggctgg
     5641 ccggaggtgt ggcgccgtcc gcatcgggca gtgccgggtc tgtgaggggc gatgtgctca
     5701 acgcgctgag cgcgcttgga tacagcgaca aggaggccgg tgccgcgctt gccaaggttt
     5761 ctcctgaagc cgctgtcccg gaggcgatcc ggcaggccct gaagctgctc tcaaaagcgt
     5821 agcgacagca atcgccagtc tgtttcatgt gcgtcggcgc ggagatcggg ctgaatgaaa
     5881 atctcgagga aatcgaccgc cgcaatgtcg ccttgaaacg acaataaaac agcacgtgaa
     5941 gacagtgatt tgcaggaacg ctgggatata atcctgctca attcatggcg ccggcatggg
     6001 gcgccgcact cagccaggag cgggttcttc atgattgtgg acacacgaca ggtcgctgcg
     6061 gctttgctgg ccttggcgat cgcgctgccc gtcgcggcaa atcccgagac cgcgagcggc
     6121 aagaacgacc aaatcgccga aatcatcgac ctgcacgatc tcaagacctc ggtttcgatt
     6181 ggcaactact acctcaagca ggaaagcctg ctggccattc gcgcgctgct agcccgtctg
     6241 ggacgggaac agaacctcgg cccggaatgg aatcccgcca agccgctctg gcagcgcgcg
     6301 gaaggaaaac tgctcggagc ggtgacggcg agaattgccg aggattgggc aagtctcgac
     6361 tggctgcggc cgcagtggca tgaccttgac aatcgcgagt tttcttccga ggaactcgac
     6421 gtgctgttgt cgcacttgcg ctccgacgtg ggccgcaagc aggtcaggat catggatcat
     6481 acggtgtcga cccacgtgat gatggccctg tcattttccg gcaagctgag agacgttgcg
     6541 ggtgtcgagg ccgatcgcac gcgcatgcag gacttgtgga acgcggagga cgcggagatg
     6601 cgtttttcca tccaggatgc ggccaacgtc gaggggcagc gctttgccct gtcgccgctc
     6661 ggaaagaagt atttcgtgac tgcgatcctg aaactgaccg gcatcgtcaa ccggcgcatc
     6721 gacgaagtgg ccggcatgct gcccaagcag gtggatgcct atgccgacca ggtccggccg
     6781 ttgctagagg aattcaagag cggtcgcggc tgatgcctca aagacgtctg cgccgcggca
     6841 ccagaggatg atcgaaaccg accgtctgat tcagcccgtc ccgatctcga cgcaggagga
     6901 agcgctcgag cgcgcgttgc gtcccaagca gctcgacgag tacatcggcc aggagaagat
     6961 ccgcggacag ttgcagattt tcatccaggc cgcgaggaac cgcagcgaag ctttggacca
     7021 cgtgctgctg ttcggcccgc cgggactcgg caagaccacg ctggcgcaca ttatcgccag
     7081 ggaaatgggc gtgaatctgc gccagacttc gggcccggta ctggaacgac ccggcgatct
     7141 ggcggcgctg ctcaccaacc tggaacccaa cgatgtcctg ttcatcgatg agatccaccg
     7201 gctctcgccg gtagtggagg aaatcctcta ccctgcgctc gaggactttc agctcgacat
     7261 catgatcggc gagggtccgg cggcgcgctc ggtgaagctc gagttgccgc cgttcacgct
     7321 ggtaggcgcc accacccgcg ccggcatgct caccaatccg ctgcgcgacc gcttcggcat
     7381 cgtctcgcgg ctggagtttt actcaccagc agagttgacg cagatcgtcg agcgttcggc
     7441 gcgcctgctc gttgtggaca tcgcggccgg cggagcactg gaaatcgcta cgcgctcgcg
     7501 cggcacgccg cgcatcgcca accgcctgct gcgccgcgtg cgcgacttcg cggaggtgaa
     7561 agcgcagggc aaagtgacca aagacgtggc cgacgccgcg ctgaagatgc tcgacgtcga
     7621 ccacatcggg ctggatgtga tggaccgcaa acttctgctt gcggtgctgg agaagttcgg
     7681 cggcggaccg gttggcatag aaaacctggc ggcggcgatc ggcgaggagc gcgacaccat
     7741 cgaggatgtg ctggagccgt tcctgattca acaggggtat ctgcaacgca cgccgcgcgg
     7801 gcgcatggcc accgtgttga cctatcggca tttcggcgcc gccctgcc
//
LOCUS       DASQOP010000208         3713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_292080, whole genome shotgun sequence.
ACCESSION   DASQOP010000208 DASQOP010000000
VERSION     DASQOP010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292080"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VE030_08480"
     CDS             complement(<1..453)
                     /locus_tag="VE030_08480"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HZF23486.1"
                     /translation="MNRFAPPVCAVAFLLAGAANAAEFDCLIEARQQVDIRSPVEAVI
                     ESVQVQRGDLVKKGQVIATLESGPERAALELARSRATMQGELKAAEARMDLAQKKMVR
                     AEELFKQNFVSVNARDEAVAEYRFSTEQLRQAKENQQLAELEVRRSSEV"
     gene            977..2716
                     /locus_tag="VE030_08485"
     CDS             977..2716
                     /locus_tag="VE030_08485"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZF23487.1"
                     /translation="MRSGPFERSPRDRRKADVLQESEARFRALVQNSFDIITIHDENG
                     MTIYESPAASRVLGYPPGALIGKTPFETLHPKDVARARDAFDALLRGQAPRSPLLLRF
                     RHADGSWIHLEALGNNLLEHPGIRGIVLTSRDITGRKRAEERAQFLANYDVLTGLPNR
                     FLMQDRLTQAVAQAHRNRLRVAVMHIDLDRFKVVNETLGHYVGDALLKQAAERIRKST
                     REGDTVARVGGDEFTIVFPNVTSLQALSTTAEKILSDLAKPFPSDGQELFVSASIGLS
                     LYPDDAATVDEIIKHSDAAMYSAKHLGRNNFQFFTTDLNQEVQERMLIEGGLRTAIQR
                     DELSLLYQPKIDLATRQVYGAEALLRWVHPKLGMISPARFVPVAEEAGLVGQIGEWVL
                     RTACRQIREWQDAGFALQVAVNVSARQFQQYDVAELVMGIMNETGALPENLELELTES
                     AVMHDAETSIVTLERLAALGAQIAIDDFGTGYSSLSYLKRLPLDVLKIDQSFVRDISS
                     DPNDAAIVRAIITLARSLGIKVIAEGVENEAQLAFLNAYGCQYAQGYLFGEPLTTRQL
                     EQMIIGQLEKKAV"
     gene            complement(2713..>3713)
                     /locus_tag="VE030_08490"
     CDS             complement(2713..>3713)
                     /locus_tag="VE030_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZF23488.1"
                     /translation="EDLEDSPSSGAAKPSPILTDGSLLDAYSNAVVSAADRISPAVVK
                     IDVRRNAKQHRSESGGSGSGFLFTPDGLILTNSHVVSGASEVGVSLRDGHSLDADILG
                     DDPHTDLALVRVSGSKLPTVQFGTSRGIRVGQLAIAIGNPYGFECTVTAGVVSALGRS
                     MRAYSGRLIDDVIQTDAALNPGNSGGPLSNSRGEVIGVNTAIIMAAQGICFATAIDTA
                     KVVVAQILRHGRVRRGWLGIAGQNMPLSRRMVRYHELEIESGVRIASVESGSPAEKAG
                     LREGDVVVSYDGTAVAGIDDVHRLLTETRVGQEAPIVVLRRGEKLVRPIVATELVR"
BASE COUNT          682 a         1214 c         1148 g          669 t
ORIGIN      
        1 cacctcgctg gagcgccgta cttcgagctc cgccagttgc tgattctcct ttgcctggcg
       61 cagttgttcg gtggaaaacc ggtattcggc taccgcctcg tcgcgggcat tgaccgaaac
      121 gaagttctgc ttgaacagct cctcggcacg caccatcttc ttctgggcga ggtccatgcg
      181 tgcttcggcc gccttcaact cgccctgcat ggtggcccgc gagcgggcca gttcgagggc
      241 cgcgcgctcc gggccggatt ccagggtggc gatgacctgg cctttcttga ccaaatcgcc
      301 gcgctggacc tgtaccgatt cgatcaccgc ttccaccggg ctgcggatat ctacctgctg
      361 gcgtgcctcg atcaggcagt cgaattccgc agcattggcg gcgccggcca ataagaaggc
      421 aacggcgcaa accggtgggg cgaatcgatt catcgggaac agggggatgt gccttggggg
      481 tacaactgtt gattgtagcg aattgacctt gttgccgtaa cacgaaattc gtaaaaaaat
      541 cagagcccgg gcggggtcta taatgaattt ggttgagctg cctcggcagc ccgcccccgg
      601 ccattctcaa gagtgtgccg ggcgctcgaa gccacgcttg gagatccctg gacctgaggg
      661 tcggaatggc caagaagatc aagcgcgatg taaaaaaatg aacctaaaat gcgccgaatc
      721 aataacaacg ccaccatagt tccagtacta taggaacggg ggagcgccgg ttcccggcct
      781 tgcgggaatc cttttcctcg gcgaaagcac aattggacaa gacgaaaaaa ataggggccc
      841 gcggccgcga tgattgcggg agcacgctga tcaactccgc cggaatgtcc gcccgtcccg
      901 ctttaggcgg gggggactcg gttgcgttgc ctcggccccg ccgttccggc gtattggcct
      961 agccgggctg accatcatgc gatccggccc tttcgaacgt tctccgcgcg accgccgcaa
     1021 ggccgacgtg ctgcaggaaa gcgaagcacg cttccgcgcg ctggtccaga attcgttcga
     1081 catcattacc atccacgatg agaacgggat gaccatctac gagtcacccg cggcatcgcg
     1141 tgtgctgggg tatccccccg gcgcgctgat cggcaagacc ccgttcgaga ccctgcatcc
     1201 caaggacgtg gcgcgcgcgc gcgacgcgtt cgatgcgttg ctgcggggcc aggcgccgcg
     1261 ttcgccgttg cttctgcgct tccgccatgc cgacggctcc tggattcatc ttgaggcgct
     1321 gggcaacaac ctgctcgagc accccggcat ccgcggcatt gtcctgactt cgcgcgacat
     1381 caccggacgc aagcgcgccg aagagcgggc gcaattcctc gccaactacg acgtgctcac
     1441 cggcttgccc aaccgcttcc tgatgcagga ccgcctcacg caggcggtgg cgcaggcgca
     1501 ccgcaaccgc ctgcgggtcg ccgtcatgca catcgatctg gaccgcttca aggtggtcaa
     1561 cgagacgctg ggccactacg tcggcgacgc gctgctcaag caggcggccg aacgcatccg
     1621 caagtccacc cgcgaaggcg ataccgtggc gcgggtgggc ggcgacgagt tcaccatcgt
     1681 gttcccgaac gtcaccagcc tgcaggcgct gtccaccact gccgagaaga tcctcagtga
     1741 tctcgccaag ccgtttccca gcgacgggca ggaattgttc gtttccgcgt ctatcggcct
     1801 gagcctgtac ccggacgacg cagccactgt agacgagatc atcaagcatt ccgacgcggc
     1861 gatgtacagc gccaagcacc tgggacgcaa caatttccag ttcttcacca ccgacctgaa
     1921 ccaggaagtg caggaacgca tgctgatcga aggcggcttg cgcaccgcaa tacagcgcga
     1981 cgagctgagc ctgctgtatc aaccgaagat cgatctcgcc acgcgccagg tctacggcgc
     2041 cgaggcgctg ttgcgctggg ttcatcccaa gctcggcatg atctcgccgg cgcgtttcgt
     2101 gccggtggcg gaggaggcgg gcctggtagg acagatcggc gaatgggtgc tgcggaccgc
     2161 gtgccggcag atccgcgagt ggcaggacgc gggctttgcg ctgcaggtgg cggtcaacgt
     2221 ctcggcccgc cagtttcagc agtacgatgt cgccgagctg gtaatgggaa taatgaacga
     2281 aaccggagcg ttacccgaaa acctcgagct cgaactcacc gagagcgcgg tcatgcacga
     2341 cgccgagact tcgatcgtca cgctcgaacg gctggccgcc ttgggcgcgc agatcgcgat
     2401 cgacgatttc ggcaccggtt actcgagcct gtcgtacctc aagcggctgc cgctcgacgt
     2461 gctcaagatc gaccagtcgt tcgtgcgcga catttcctcg gacccgaacg acgcggcgat
     2521 cgtgcgcgcc atcatcacac tggcgcgcag cctcggcatc aaggtgatcg ccgagggcgt
     2581 cgagaacgag gcccagctcg ccttcctgaa cgcctacggc tgccagtacg cgcagggtta
     2641 cctgttcggg gagccgctca ccacccggca gctggaacag atgatcatcg ggcaattgga
     2701 gaaaaaagcg gtttagcgta ccagctcggt cgcaacgatc gggcgcacca gtttctctcc
     2761 acggcgcagc accacgatcg gtgcttcctg cccgaccctg gtttcggtca gcaggcggtg
     2821 cacatcgtcg attccggcca ctgccgtgcc gtcgtagctc accaccacgt cgccttcccg
     2881 cagtcccgcc ttttccgccg ggctgccgga ctccaccgag gcgatacgca cgccgctctc
     2941 gatctcgagc tcgtgatagc gcaccatgcg ccgcgacaac ggcatgttct gcccggcgat
     3001 gccgagccag ccgcgccgga cccggccgtg ccggagtatc tgtgcgacca ctactttcgc
     3061 ggtgtctatc gcggtggcga aacagatgcc ctgtgccgcc atgatgatcg cggtgttcac
     3121 tccgatcacc tcgccgcggg agttgctcaa cggcccgccg gagttaccgg gattcagcgc
     3181 ggcgtcggtc tggatcacgt cgtctatcaa ccgcccggaa taagcacgca tcgaacggcc
     3241 gagcgcgctg accactcccg cggtgacggt gcattcgaag ccgtatgggt tgccgatggc
     3301 gattgcgagc tgtcccacgc ggatgccgcg cgaagttccg aactgcaccg tgggcagctt
     3361 cgagccggac acccgcacca gcgcgagatc ggtgtgggga tcgtcgccga ggatgtcggc
     3421 gtccaggctg tgcccgtcgc gcagactgac cccaacttca gacgcgccgc tcacgacgtg
     3481 gctgttggtg aggatcaatc cgtccggagt gaacagaaaa ccggaaccgc tgccgccgct
     3541 ttcgctccgg tgttgcttgg cgttgcgacg cacgtcgatt ttcaccaccg ccgggctgat
     3601 cctgtccgca gccgatacca ccgcgttcga gtaggcgtcc agcagactgc cgtctgttag
     3661 aatcggcgac ggttttgccg cgcccgaaga cggggagtcc tcgaggtctt cta
//
LOCUS       DASQOP010000209         2233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1903691, whole genome shotgun sequence.
ACCESSION   DASQOP010000209 DASQOP010000000
VERSION     DASQOP010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1903691"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..1018)
                     /locus_tag="VE030_08495"
     CDS             complement(167..1018)
                     /locus_tag="VE030_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23489.1"
                     /translation="MNEVGPKLDELERVLRGIGPVAVAVSGGVDSLTLAAFAHRALGE
                     DVQMFHAVSPAVPHEATVRVQKLADGEGWALTVIDAGEFDDADYRSNPVNRCFYCKTN
                     LYGAVAPRTTAQIVSGTNLDDLGEYRPGLEAAKNYGVRHPFVEAGIDKNTVRAIARHL
                     DLAEVAELPAAPCLSSRIETGIAIDPGMLKLVHAAERLVSQALTPRTVRCRVRAKGVV
                     VELDEQNLAALDSSRRQSLSAQVGRLFSDGGFDYPVSLAPYRSGSAFLVRMASPPALS
                     TSGEGAN"
     gene            complement(1015..>2233)
                     /locus_tag="VE030_08500"
     CDS             complement(1015..>2233)
                     /locus_tag="VE030_08500"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LarC family nickel insertion protein"
                     /protein_id="HZF23490.1"
                     /translation="NPVMHIHLDAVGGVAGDMFIAAMLDAFPDLRDGMVSAIRAAGLP
                     AQVHVGVAEHCDHALTGLRFRVTEPLEAGALLGAHHHPHTPFAEIRAHLQASALEPAV
                     KEHAVGVFTVLAEAEARVHGKTVDTVSFHEVGEWDSIADVVGAAFLIDALAPATWSVG
                     ALPLGSGRVRSAHGSLPVPTPATVLLLEGFEFVDDGLDGERITPTGAAILRHLDCAQG
                     GDRKQRKLLRTGIGFGNRSFPGLSNVLRVLAFEEARTGSGGDEIAQILFEVDDQTPED
                     LAIAIDKLRAQPSVLDVLQTPAFGKKGRMTMHVQVLADPADLENVFDACFNETTTIGL
                     RYQVLQRRKLSRAGRTVEAGGRQVRVKISERPGKQTAKVEADDLLNVIGGRAGREQLR
                     REAEQVVLRKERK"
BASE COUNT          383 a          782 c          685 g          383 t
ORIGIN      
        1 gtcatgtcaa tccacccacc tcaccccaac gcgcggacac gttaccgaca aagtcggccg
       61 attgcgggta acgcgttatt gtttcgcctc ccgcccctct tgctccccgc aactgccgag
      121 tcggcgaaac ctctcccccc gcaagcgggc ggagagggtg caagaatcag ttggctccct
      181 ctccacttgt ggagagggcg gggggtgagg ccatcctgac caggaaggcg ctgccgctgc
      241 gataaggcgc aagcgacacc ggatagtcga aacctccgtc gctgaacagc cggcccacct
      301 gtgccgacag cgactgccgc cgcgatgaat cgagcgccgc aaggttctgt tcatccagtt
      361 ccaccactac accctttgcc cgaacccggc aacgcaccgt acgcggcgtc agtgcctgcg
      421 acacaagccg ctccgccgcg tgcaccagct tgagcatgcc cggatcgatc gcgattccgg
      481 tttctatccg gctcgacagg cagggtgccg ccggcagttc ggcgacctcc gccagatcga
      541 gatgccgcgc aatcgcgcgc accgtgttct tgtcgatacc ggcctcgacg aagggatggc
      601 gcacgccgta gttctttgcg gcctcgaggc cgggccgata ttcgccgaga tcgtcgaggt
      661 tggtgccgga gacgatctgc gcggtggtcc gcggcgcaac cgcgccatac aggttggtct
      721 tgcagtaaaa gcagcggttg accgggttgc tgcggtaatc cgcatcgtcg aattcgcccg
      781 cgtcgatcac cgtcagggcc cagccttcgc catccgccag cttttgcacc cgcacagtgg
      841 cttcgtgcgg aacggcaggc gacaccgcgt gaaacatctg cacatcctcg ccgagagccc
      901 ggtgcgcgaa cgccgccagc gtgaggctgt ccaccccgcc gctcaccgcg accgcgacgg
      961 gaccgatgcc gcgcagcacg cgctccagtt catcgagctt gggtccgact tcgttcactt
     1021 gcgttccttt ctcaacacaa cctgctccgc ctcccgccgc aactgctcgc gcccggcgcg
     1081 gccgccgatc acgttgagca ggtcgtcggc ctccaccttg gcggtctgct ttcccggccg
     1141 ttcggagatt ttcacccgga cctgccttcc gcccgcttcc accgtcctgc cggcacggga
     1201 tagtttgcgg cgctgcagga cctggtagcg caagccgatg gtcgtcgttt cgttgaagca
     1261 agcgtcgaat acgttttcca ggtccgccgg atcggcgagc acctggacgt gcatggtcat
     1321 cctgcctttc ttgccgaaag ccggcgtctg caacacgtcg agcaccgacg gctgcgcgcg
     1381 caacttatcg atggcgattg ccagatcctc cggcgtctgg tcgtcgactt cgaacaagat
     1441 ctgcgcgatt tcatcgccgc ccgacccggt gcgcgcctcc tcgaaggcca ggacgcgcag
     1501 cacgttgctg agaccaggga agctgcgatt gccgaaaccg ataccggtgc gcagcaactt
     1561 gcgctgcttg cgatcgccac cctgcgcgca atccagatgc ctgaggatcg ctgccccggt
     1621 cggcgtgatg cgctcgccat cgagtccgtc gtcgacaaat tcgaaacctt cgagcaacag
     1681 cacggttgca ggggtaggca ccggcagaga accatgcgcg cttctgaccc ggccgctgcc
     1741 cagcggcagc gcgcccaccg accaggtggc aggcgcaagt gcgtcgatga ggaaggcggc
     1801 accgacgacg tcggcgatcg agtcccattc gccgacttcg tggaagctca ccgtgtccac
     1861 ggtcttgccg tgcaccctgg cttcagcttc cgcgagcacc gtgaaaacgc cgactgcgtg
     1921 ctccttcacc gctggctcaa gcgcggacgc ctggaggtgc gcacggattt cggcgaacgg
     1981 cgtgtgcgga tgatgatgcg cgccgagcag cgcgccggcc tcgagcggct ccgtgacgcg
     2041 gaaacgcagc ccggtcagcg cgtggtcgca atgctcggcc accccgacgt gcacttgggc
     2101 aggcaggccg gcggcacgga tcgccgaaac catgccgtcg cgcagatccg gaaacgcgtc
     2161 gagcatcgcg gcaatgaaca tgtcgcccgc cacgccgccg accgcgtcta aatgaatatg
     2221 catcacgggg ttg
//
LOCUS       DASQOP010000210        23882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2108820, whole genome shotgun sequence.
ACCESSION   DASQOP010000210 DASQOP010000000
VERSION     DASQOP010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 23882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23882
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2108820"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VE030_08505"
     CDS             <1..558
                     /locus_tag="VE030_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZF23491.1"
                     /translation="VKQMKLPPLGIRFVFPHAPMRPVTINGGFVMRAWYDIAYQELAF
                     KEDERGLRDSQKLIEDLIARENKRGIPSSRIVLAGFSQGGVITLQAGLRQPKRLAGLM
                     ALSAYLPMSPMVEVERNAASNSVPIFMGHGIADDIVPLALAKMSRDTLIKLGYEVEWH
                     QYQMPHSVCPEELADIGAWLKRVLH"
     gene            complement(774..2312)
                     /locus_tag="VE030_08510"
     CDS             complement(774..2312)
                     /locus_tag="VE030_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23492.1"
                     /translation="MLNWIKGTQSDHPLAEDKAARELIAELPANDSFKALEELSYWLD
                     TLKGAEDLKLGRQFEIIDLIDQAAKNHQRKLSQDYLAGSARLQKFQEIRIWNTSFSFW
                     KQLSDAYQLCLTRYRAGAGGWSALKGPSPTIVARAMRALSLQLKWQLLRYGPVAPRVW
                     EEYGKLFAYAEEKGFTAAKVEVYPGKFGESTVQREFLKGLMLAISSTDSLLPMKLEVA
                     EGVVAQFSESFVIQRQPGKGCHYFIDISTGKGPARMVSRLQMTPGLRFFGPGSAAQEL
                     EKLITVLQADGAVPSNVNLGGVFDPEVVLEVLRHLARYWAAVPPARGEERRRSVSRIS
                     VIHDFDEILATISSDTEDLAFDNSAETWTVENESAGGYGALLPPAKGDWLKVGTLLGV
                     KLEHGAAWGVGIVRRLSAYDLKQRYVGIQVFSKGATVVKIAPANPGGSAGAQDAVLLP
                     SSTSDSSGTGEMSLLLRPGVFSEKKTYEMQAYGRRYLLVPKKLLEGGDDFDMARFRVM
                     QHAS"
     gene            complement(2410..3726)
                     /locus_tag="VE030_08515"
     CDS             complement(2410..3726)
                     /locus_tag="VE030_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZF23493.1"
                     /translation="MPVPSSKKNLIDDVRPREVWGWMMFDFANSGYTTVVITAVFNAY
                     FVAVVAGQAVWATFAWTAALSVSYALIVVSAPLIGAYADAHAAKKRLLALTTIGCVAG
                     TAALALVGPGDLALGVALLIISNFFYGTGENLIAAFLPELAKGRSLGKVSGWGWGLGY
                     IGGLVSLGASLAYVTWAQAQGQSAAEFIPVTMLITAALFAVSCLPTFLLLKERARPQQ
                     LPTGAYASAFARTWETLKHASRYRDLMRFLLCILFYQAGVQAVISLAAIYSEQEMKFS
                     TQQTIMLILVVNITASVGAVAFGQWQDKLGHVRTLALTLIGWCLTVVLAGLATTPALF
                     WVAANLAGVCLGASQSAGRALVGYLSPPDRRAEFFGLWGLAVKLSSILGPITYGLVSW
                     ISAGNHRLAILITGAYFLVGLALLAGVNARRGHRAARPLKSARQLQ"
     gene            complement(3794..5071)
                     /locus_tag="VE030_08520"
     CDS             complement(3794..5071)
                     /locus_tag="VE030_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23494.1"
                     /translation="MEYSEAVYSDIIITWLGAAGVWGYLNFVHHHRTGGALERNTLFL
                     LYCAFALMTLRGFYWLLRYPWLDYLRFFPTAVLPLAIVLFAESIVRRHVSLPMKIYAA
                     GGTVFFVLMTLLGQLHSGKILVIAFLCFEVSIIIAMAWMVWRADQTKLTTLEIRLNNA
                     IFVSAMFAVPLVITDFREELTWIPRRLGAAGALIFVYVLVRMSYRVDTRIIVINEVLN
                     IFLRASIMALSFMAITDNLTVREFFEFFPLSLMFVLVFTIFYRLKAVAVESRGASFLQ
                     WLLHAKMTGLENFVQSLRHLPLTQENVLLRMDDLERYDAAAIIEYLGAERRVLSLTVL
                     RERAESQESGSAEIFEQLVDILERHAMSHIGLASLKPPALLLFNSPLIPGAHFDEIKI
                     NLILKLCRILPQAPKAKEAPRQDAGAVTTIGTQ"
     gene            complement(5094..6032)
                     /locus_tag="VE030_08525"
     CDS             complement(5094..6032)
                     /locus_tag="VE030_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23495.1"
                     /translation="MIHLHKLGRKYLDFEFPLKNEVRLRIVERPGLWMGRGALDHLLA
                     DVRQVVKTVIPSGLEYGVLSGDKRRLDNAVLSILYDQASRKPIAFNAYSFLDVEVHGR
                     RVNVLHLGLIMVDPSFQAQGYTWVVVGLPCLLVFLRSGMGPVWITSVTQVPAIVGKVA
                     EAFADVYPSPSGKTRRSFAHLTIARQVMESHREVFGVGLQAQFDQDNFIIRDAYTGGS
                     DSLKKAYDETTKHRDDVVNEMCRDRLDYRRGDDFLQVGAINFACACDYLLRTVPRDSL
                     PSLLYRVMFVLAGRLLLPLWHWFSPNRQWRDLRPRR"
     gene            complement(6084..6758)
                     /locus_tag="VE030_08530"
     CDS             complement(6084..6758)
                     /locus_tag="VE030_08530"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZF23496.1"
                     /translation="MLEDGSHGSAPVTRKSRSRLRRERDKQRMRERVLDAARELFAVH
                     GYEAVTMRKIAERIEYTTTMIYQHFPDKETLIREIVAHDFQSFGQEFRRCAQIADPIA
                     RILAMARVFVDFGVQHPNHYRLMFMSPPSSEMKEANLAAIQADPAQDVYTFFKAAIEE
                     AMRKGLLRPELDDAELLVQSLWSGMHGVVSLHIAKGRDPWIEWRSLDRISSLLVELLL
                     RGLKKD"
     gene            complement(6837..8240)
                     /locus_tag="VE030_08535"
     CDS             complement(6837..8240)
                     /locus_tag="VE030_08535"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZF23497.1"
                     /translation="MSGAATKLMVGGRGGGACAQRPLPAPVRAWRMLRIAALFLLFAL
                     FTAVVTAFASVRRRERFTRWWAGRVLRALGVTLICGRSPGFGTGPALLVANHVSSIDP
                     ILICAIQPACFVAKSELGNSAWLRWLLAEAGTIFIDRGKRDGVLSALAAMHDAFDAGE
                     QVALFPEATICAAAEPRPFNSALLEAGSEGVPLYFFALSLTRADGTLCPEADFPWERS
                     FAAGFWRLAGVPALVARVEVVGPVDFQGVNRKRIARSAHDAIHYALRYRHAGRCSCPR
                     ALRAWGSRIAQSAIAASLCYALVQGPVLAEGVDIGAGFGLVSQQHGLEGGWDIQVGYE
                     FKETERFSLGVQWQWFKGWTSENRLGDHDQGGMAFSSNALYLTARPRHEALRWLQLKA
                     GLVNANYKIAEYDSAAGTVVVRSDRSGETGLALGLGLVFGSDKVRLHLLDYQRYSFAG
                     ESFDAFGISLVLLAGGH"
     gene            complement(8237..9022)
                     /locus_tag="VE030_08540"
     CDS             complement(8237..9022)
                     /locus_tag="VE030_08540"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HZF23498.1"
                     /translation="MRSREGSIGTRILGTASVRAAYRIVLAEDAHGVRQAQRLRFEVF
                     NLELGVGLARAYGAGLDEDEFDPRCDHLLLEHCDSGTIVSTYRLLPGRRVVTVASLYS
                     ATEFDLTAFGPLLPELGELGRACIQRRHRNPTALGLLWRGIAEYAASHGLRYLIGCSS
                     LHTVDAAVGAALYRDLAWQYLAPERFRTRPRSGYACPLETSAGVSVDPPKLLSAYLSL
                     GARICGAPAIDREFGSIDFPTVLDLETLGPRLHRYAAGTEGDT"
     gene            complement(9033..9623)
                     /locus_tag="VE030_08545"
     CDS             complement(9033..9623)
                     /locus_tag="VE030_08545"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZF23499.1"
                     /translation="MTTEEYLIYFALGVVLFVAGHQVYFWCQRYPLLPPRVFRPAIDE
                     LIPYRPEWVWIYSILYYAVIVAVFFIVDSERKFLLLASSYGLLLAAQMLFFLAFPVAT
                     PRDWRKRNLRRTWSEKLLALVQKADMRSNSFPSMHTSVATLTALHLYPLFGELVLAFP
                     VLIGLSCLFTKQHYVMDVPTGASLGYAIFMISQMIA"
     gene            complement(9652..9840)
                     /locus_tag="VE030_08550"
     CDS             complement(9652..9840)
                     /locus_tag="VE030_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23500.1"
                     /translation="METTVAVNQIATQDNRFDVLGADSGFEMDPDDSVCDAYEFLGAV
                     GFARGEAYSLAVDMIELS"
     gene            complement(9885..11735)
                     /locus_tag="VE030_08555"
     CDS             complement(9885..11735)
                     /locus_tag="VE030_08555"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HZF23501.1"
                     /translation="MSIAMTLDAKPAGVIPAMNAKGALVAGENLLVAFALAALALLPV
                     AEILLRAVFHVGIPGAATLTQHLVLIAGMLGAAIAARENRLLSLSSLPLILRGWTRSA
                     AHALSGCVGALVSALLAVASTQLVITEKLAGNLLVEGLPVWVVQAIMPPGFALIAVRL
                     VLHSGEGWRIRAACAAVALCAAVLAAYGPLSAEALRFPALIGLLVATILGAPVFTTLA
                     GAALILFWSDGLPIASIPIDLYRLTVNPSLPAIPLFTLAGYFLAEGGSPARLVAVFKA
                     LVGGLPGGSVIVVVATSTLFTAFTGASGVTILALGGLLMPLLLATRQDEGRALGIITS
                     AGSLGVLLPPSLPLILYAVVARVPMEDMFLAALLPALLMAVILAVYAARHEVRNAAAR
                     FDPVQAWRAISEAKWELLLPVVAFVALFGGFATPVEAAAMTATYAFMTAALVRRELHI
                     VRDVPRVVAECGLLVGGVLLILGVALGFTNYLVDAQLPDRMVEWATGTIESRWVFLLA
                     LNGFLLVVGCLMDVFSAIVVVAPLLIPVGLAFGIDPLHLGVIFLANMELGFLTPPVGM
                     NLFFASYRFGKPVAEVCRAVLAYFLVMAMGVIAITYLPALTTWLPAMFRG"
     gene            complement(11732..12790)
                     /gene="dctP"
                     /locus_tag="VE030_08560"
     CDS             complement(11732..12790)
                     /gene="dctP"
                     /locus_tag="VE030_08560"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HZF23502.1"
                     /translation="MNDIARIDTQRRRLCFAAGVATALPALTLRHQAQAADAMRIRLG
                     TLVPKGSSYHRALQEMGERWRKAQGAGATFTIFADGTQGGEADVVRRMRVGQLNAGLL
                     SVIGLLEIDRSVMALQYMPMVYRNWDEVDYVRERLGPTLEARLLEKGFVVLFWGDGGW
                     VRYFSNAPALRPADFRRMKMFVWSGDPQQVEIMKSMNYQPVPIETADILPGLQTGLID
                     AVPAAPFFALAGQFNGPAPYLLDVKWVPIVGACVVTRKAWDAMSETAREELKRAAAQA
                     GAEIRSRGRAEDLESIEAMKRRGLKVATMTPEIEAEWRRTAEEVYPRIRGTAVPAPIF
                     DEALRLVAEFRAAGLARK"
     gene            complement(12801..13760)
                     /locus_tag="VE030_08565"
     CDS             complement(12801..13760)
                     /locus_tag="VE030_08565"
                     /inference="COORDINATES: protein motif:HMM:NF028121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HZF23503.1"
                     /translation="MLASLSNWFGEWVFSGGGWTRLVLLLGLMIGLLPGCSVRQLAIN
                     KAGDALASSGIVFAADDDPELIRDAAPFSLKLMESLLAESPRHRGLLYATSSGFTRYA
                     YAFVHQDADRLEDQDLKAASEQRSRAQRMYRRARDYGLRGLDVAHPEFSRKLRLDPKS
                     AARIARKDDVPLLYWTAVSWAALISLSKHDPDLVADVPVVEALIDRAAVLDEAYDSGA
                     IHNFLITFEMVRQGATGDPATRARHHFDRAVSLTGGLQAGPYVSLAESVAVPRQDRSE
                     FEALLKQALAIKPGQRPEWRLANTVMQRRARWLLAQSDQLFAE"
     gene            complement(13992..14444)
                     /locus_tag="VE030_08570"
     CDS             complement(13992..14444)
                     /locus_tag="VE030_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZF23504.1"
                     /translation="MMLVKDILRIKGGQIFSIAPDALLPQAVGLMVERDIGSLLVMEK
                     GKMTGLLTFREVLAAVNRHRGDIHEVRVEEVMVMEPICANPDESADHMRTIMTDQHIR
                     YLPIMENGALIGVLSFHDVAKAALRAVNFENKLLKQYIKDWPEGDKAS"
     gene            complement(14493..15680)
                     /locus_tag="VE030_08575"
     CDS             complement(14493..15680)
                     /locus_tag="VE030_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010396205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HZF23505.1"
                     /translation="MGPLSDIKVLELGTLIAGPFCSRIFAEFGAQVIKIESPDGGDPL
                     RQWRKLHNGTSLWWYLQARNKKSVTVNMREPEGQEIVRKLAAGADIVIENFRPGALEK
                     WGLGWDRLAQLNPGLVMVRLSGYGQTGPYRERAGFGAIGEAMGGLRYVTGYPDRAPVR
                     VGISLGDAVASLYGVIGALMALRHREVNGGRGQVVDVALYEAVFSLMESLLPEFDMTG
                     FVRERSGASLPGIVPSNTYTTRDGKYVVIGANGDAIFRRMMTAIGREDLGNDPSLARN
                     DGRVARTAELDEVIGDWCAAHDLGHVLEVLAAAQVPSGKIYDIADIVKDLHYQARGMI
                     EQARLPDGEPMKVPGVVPKLAQTPGGTRWLGPALGEHTGEVLAHLGYDAARIADLKSR
                     GVI"
     gene            15797..16351
                     /locus_tag="VE030_08580"
     CDS             15797..16351
                     /locus_tag="VE030_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HZF23506.1"
                     /translation="MKRSPALLAALLFLTLISEVSMAACPDLLNHQMNNLDGKPVNLC
                     DYAGKVVLAVNTASYCGNTPQYKGLESLYQKYRDKGLVVVGFPANDFGSQEPGTAKEI
                     KDFCELTYGVKFPLMEKSSVVPGKANPVFQELTRMTGDAPEWNFHKYLIARDGKRAFS
                     FAARTQPETGEIVKQIEALLAEKP"
     gene            complement(16588..18264)
                     /locus_tag="VE030_08585"
     CDS             complement(16588..18264)
                     /locus_tag="VE030_08585"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZF23507.1"
                     /translation="MHDLIIENATIYDGLGSAPRRGSVAVTDGRIAEVGKVEGEARER
                     VNADGLALMPGIIDTHTHYDAQITWDPFANPSPALGVTTVVMGNCGFTIAPCRPPDRD
                     LTMRNLTHVEGMSLEALRAGIDWGFESFPEYVGMLEKNGVGPNVACFVGHSGVRTFVL
                     RGDAAKRAASPDEIAQMRRIVIEAMEAGACGFSTTRSYQHNGENGIPMPSRLADEHEF
                     LALSGALREGGKGLLMMTKASDTPVPWIESLAAAADRPYLVAALLHSNLTPEASFEDL
                     EHISRARGRGRRMYGAVSACPLNFEFTMHEPYVFEGLAAWKPLMKMEELEVKKALGDP
                     AFRIRFKDELSKRARRMFTGEWEKAFVAKVADPAHADYEGQSIGALARKQGAHPLDFM
                     LDLALSEDLDTLFTATLLNSNEDAVGRMLNDPNAIVSLSDAGAHLTFLCDAGFGLHLL
                     GHWVRERKLMPLEIAVKKLTSEPAQLLGIRGRAAITAGNWADLLLFNPHTVGRAPSQR
                     VYDLPAGGSRIHTPGIGVHGVWVNGELVADSNGILKDAPLAGSVLRDFSA"
     gene            complement(18512..19831)
                     /locus_tag="VE030_08590"
     CDS             complement(18512..19831)
                     /locus_tag="VE030_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZF23508.1"
                     /translation="MSEFSRRKALGAMGAAVISGAASALIGPHRAHAADLSYQPEKGA
                     ELRVLRWKRFVQGDEEQWLANSEKFYKATGVKVRVDSENFEDIRPKAAVAANVGSGPD
                     IVLGWYDDPHQYADKLLDLTELANYLDAKYGGWYDVVRRFCIHDGKWIAIGSGFLGGC
                     LVYRESAVKAAGFDKVPNDTQGFLRLCQALKKKGTPCGFALGNAVGDANAWTHWALWA
                     HGGRMVDESNRVVINSKETAAALEYAAALYQTFIPGTLSWLDPNNNKAFLSGDISLTL
                     NGVSIYYAVKTSKEPGVQKLLADIQHVNMPIGPVGHAAELNPFSPMFVFKYTKYPNAA
                     KEYLRFMMERSQYEAWQSASLGYVQQPLKAYADTPVWKSDPKLLPFRNVPGLTRDNGY
                     AGKLGSASAGAMADYIVVNMFAQAASGASTIKSAMEQAESRAKRYYR"
     gene            complement(20003..20506)
                     /locus_tag="VE030_08595"
     CDS             complement(20003..20506)
                     /locus_tag="VE030_08595"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HZF23509.1"
                     /translation="MSRAFVKDDNEALPSEELPERPVNSEPNYITPAGLAKLRRKVEK
                     LHDEHVRLKGAQEDFDRPRLAQVERDLRYYQARLESAILVDVSKQPRDEVHFGASVKT
                     EDEGGAVHSIAIVGEDETDAAHGRVSWQSPLAKALMGAKVGDTVTWNRPAGSTTLNVL
                     EIHYPRT"
     gene            complement(20643..22166)
                     /locus_tag="VE030_08600"
     CDS             complement(20643..22166)
                     /locus_tag="VE030_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23510.1"
                     /translation="MMRIRSRTRDRVAARRGRTCLVAVFVLALVTGCATLQNPIRANL
                     SDANEAVAQCAAWYRAMDQRIDQAGVRDGGEYRIPGFPYLRTDRFGASFRIEALAGED
                     LFQAWTGRLLRLEAEARAAELSNLPAGSLEDLAANRAQAGERTQACATTLAGHDFAVP
                     ERRTLLAARARVPDDYVTWQRALGLYAITRIPFWYGVDGWQKEAAETMRRSKEDMRGP
                     ATLSRYAPPDAAGTQAAKEILAGARRDPLGIPQLSAEQQEALFAAFAPVIEVETGGGY
                     DRIGALAWGEGKRPVVDTSRPVVYRKLALTRYGGETLVQLVYLAWFSERPRAKSLDLL
                     AGRLDGIFWRITLSTHGDPLVYDTIHPCGCFHMFFPVPGVEPLPPPATGIEWAFVPDR
                     APKLPDGARLVLRLATRTHYLVNMAADGGGAGLTYEFADYDELRSMPAGAGKRRSAFR
                     PDALVPGTERGERVFFWPMGVPSAGAMRQWGHHATAFVGRRHFDDVDLIERRFRLLP"
     gene            22377..>23882
                     /locus_tag="VE030_08605"
     CDS             22377..>23882
                     /locus_tag="VE030_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZF23511.1"
                     /translation="MTKGKLLLMVLIAALIAAFIVFDLGQYFSLSYLKSQQAAIEELY
                     RAKPLQTVAAFFLIYVAATGLSLPGATILTLAAGAIFGLLWGTVIVSFASSIGATLAF
                     LVSRFLLRDTVQAKFGEKLKAIDAGVDRDGPFYLFTLRLVPAFPFFVINLVMGLTSIK
                     TWTFYWVSQLGMLAGTIVYVNAGTQVAQIESAAGILSPALIASFTLLGIFPLIAKKIV
                     AMIKARKVYSRFTRPRKFDRNVIVIGAGSAGLVSAYIAAAVKAKVTLIEKDRMGGDCL
                     YTGCVPSKALIRSAKLLSHIKRAREFGLREATADFQFADAMERVQRVIKAIEPHDSVE
                     RYTSLGVECIKGEAKIISPWEVQVNGKTLTTRSIVIAAGARPFVPPIPGVENVGHFTS
                     DTVWNLRKLPARLVVLGGGPIGCELAQCFARLGSQVTQVEMLPRIMIREDPEFSEMVL
                     ARFREEGIDVRLEHKAKQFLVENGEKALICEHKGREVRIPFDEVLVAVGRIA"
BASE COUNT         4583 a         7776 c         7274 g         4249 t
ORIGIN      
        1 gtgaagcaga tgaagcttcc gccgctggga atccgcttcg tgtttccgca cgcgccgatg
       61 cgcccggtta ccatcaacgg cggcttcgtg atgcgcgcct ggtacgacat cgcctaccag
      121 gaacttgcct tcaaggaaga cgagcgcgga ctgcgcgact cgcagaagct gatcgaggac
      181 ctgatcgcgc gcgagaacaa gcgcggcatt ccttccagcc gcatcgtgct ggcgggattc
      241 tcccaaggcg gggtgatcac gctgcaagcc gggctgcgcc agccgaagcg gctggccggc
      301 ctgatggcgc tgtcggccta tctgccgatg tcgccgatgg tcgaggtcga gcgcaatgca
      361 gcaagcaact cggtgccgat cttcatgggc cacggcatcg ccgacgacat cgtgccgctg
      421 gccctggcca agatgtcacg cgacactttg atcaaactgg gttatgaggt cgaatggcac
      481 cagtaccaga tgccgcactc ggtctgccct gaggagctgg cggacatcgg cgcctggctc
      541 aagcgcgtcc tgcactaaat caacacctca ccgcaccaag tcaacacctc accgcagagg
      601 cgcaaaggcg caaagggaac gcagagaaac ccaaacggga tgttgaatgc cgcgagcgaa
      661 ccgtcatcgg acaaggtgtg agaactcaga aatgctccaa ccagctatta ccattctttc
      721 ttgctcttct ttgcgtttcc tttgcgcctt cgcgcctcgg cggttaagcg gttttaggaa
      781 gcgtgctgca tgacgcggaa acgggccata tcgaaatcat cgccgccctc gagcagcttc
      841 ttcggcacca gcaggtacct gcgaccgtag gcctgcatct cgtaggtctt tttctccgag
      901 aagacccccg gtctcaacaa caggctcatt tctccggtgc cgctgctgtc gctcgtgctc
      961 gaaggcaata acacggcgtc ctgggcgccg gcactgccgc cggggttggc gggcgcaatc
     1021 ttcaccacgg ttgcgccctt gctgaacacc tggatgccga catagcgctg cttgagatcg
     1081 taggctgaaa gccggcgcac gatacccacg ccccaggccg cgccgtgttc gagtttgact
     1141 cccaacagcg ttccaacttt gagccagtcg cccttggccg gcggcagcag tgctccgtaa
     1201 ccgcccgcgc tttcgttctc caccgtccag gtttcggccg aattgtcgaa tgccaggtcc
     1261 tcggtgtcgc tggatattgt ggcgagaatc tcgtcgaagt cgtgaatcac gctgatgcgc
     1321 gacaccgaac ggcgacgttc ctccccgcgc gccggcggta ccgccgccca ataacgcgcc
     1381 agatgccgca gtacttccag caccacctcc ggatcgaaaa cgccaccgag attgacattg
     1441 gacggcaccg cgccgtcggc ctgcagaacg gtgatcagtt tttccaactc ctgcgcggcg
     1501 gagccggggc cgaagaatcg cagtcccgga gtcatctgca ggcgcgagac catccgcgcc
     1561 ggccccttgc cggtggaaat gtcgatgaag tagtggcagc ctttgcccgg ctggcgctga
     1621 atcacgaagg actcggagaa ctgcgccacc accccctcgg caacttccag tttcataggc
     1681 agcaggctgt cggtggacga gatcgcgagc atcagtccct tgaggaactc gcgctgcacc
     1741 gtcgattcgc cgaacttccc cggatacacc tcgaccttgg ccgcggtgaa gcctttttcc
     1801 tcggcatagg cgaagagctt gccgtattcc tcccacaccc ttggcgccac cggcccgtag
     1861 cgcagcaact gccatttgag ctgcagcgag agcgcccgca tcgcccgcgc cacgatcgtc
     1921 ggcgacggac ccttcagcgc actccagccg ccggccccgg cccggtagcg cgtcagacac
     1981 aattgatagg cgtccgaaag ctgcttccag aagctgaacg acgtgttcca gatacggatt
     2041 tcctggaatt tttgcagccg cgcgctgccc gccagatagt cctgggaaag tttgcgctga
     2101 tggttttttg cagcctggtc gatcaggtcg atgatttcga actgccgccc gagcttgagg
     2161 tcttccgcgc ccttgagcgt atccagccag taggaaagtt cttcgagcgc cttgaaggaa
     2221 tcgttcgccg gcaattcggc gataagttcg cgggcggcct tatcctcggc cagcggatgg
     2281 tccgactgcg tccccttgat ccagttcagc atgtgaccac ccctcgcgcc ctggcgcatg
     2341 cgtaaaacct gcctgggcga aattataacg cgctgtttat cttggtctat taattcctga
     2401 aactgccgac tattgcaact gccgcgcgct cttcagcgga cgcgcggcac gatgcccgcg
     2461 gcgtgcgttg actcccgcca gaagtgccag cccgacaagg aaataggccc cggtaatgag
     2521 gatggcaagg cgatgattcc cggcggaaat ccaactcacc aggccgtagg tgatcggacc
     2581 cagaatggag gacagtttga ccgccagccc ccacaatccg aagaattcgg cgcggcggtc
     2641 cggcggactc aggtagccga ccaatgcgcg ccccgcggac tggctggcgc caagacagac
     2701 tccggcgagg ttcgccgcca cccagaacag ggccggggta gtcgccaatc cggccagcac
     2761 cacggtaagg caccagccga tcaatgtcag cgccagcgta cgcacatggc cgagcttgtc
     2821 ctgccactgt ccgaaggcga ctgcgcccac gctggcggtg atgttcacca ccaggatcag
     2881 catgatggtc tgctgtgtcg agaatttcat ctcctgttcg gaatagattg cggcgagcga
     2941 gatcaccgcc tgcacgccgg cctgatagaa caggatgcaa aggagaaaac gcatcaggtc
     3001 gcgatagcgg ctggcatgct tgagcgtttc ccaggtgcgt gcaaacgcgc tcgcgtacgc
     3061 gccggtgggc agttgctgcg gccgtgcacg ctctttgagc agaaggaagg tgggcagaca
     3121 cgagacggcg aaaagcgccg cggtgatcaa catggtgacc ggaatgaatt cggcggcgga
     3181 ctgcccctgc gcctgcgccc aggtgacgta ggcgagcgat gcgccgagac tgaccagccc
     3241 gccgatgtag cccagccccc aaccccaacc cgacaccttg cccaacgaac gccccttggc
     3301 gagctcggga agaaatgccg cgatcaggtt ctcgccggtg ccgtagaaga aattggagat
     3361 gatcagtagc gcgacgccca gcgccaggtc tccgggaccg accagcgcca gcgccgccgt
     3421 tccagccacg cagccgatcg tcgtcagcgc cagcagccgc ttcttggccg cgtgggcgtc
     3481 ggcataggcg ccgatcagcg gcgcgctgac cacgatcagc gcataggaca ccgacagggc
     3541 cgcggtccag gcgaaggtgg cccataccgc ctgacccgcc accaccgcca cgaagtaggc
     3601 gttgaacacc gcggtgatga ccaccgtggt gtaaccggag ttggcaaagt cgaacatcat
     3661 ccagccccac acttcgcggg gccggacatc gtcgatcagg tttttcttcg agctggggac
     3721 cggcaagatt ttgcgtgcgg cggttgaggt cggaaagccc agccgggact ctaaccgcgg
     3781 aacgcgcgaa acgctactga gtgccgatgg tcgtcaccgc gccggcgtcc tgccgcggcg
     3841 cttccttcgc ctttggcgcc tgcggcagga tgcggcacag cttgagaata aggttgatct
     3901 tgatctcgtc gaaatgcgcg ccgggaataa gcgggctgtt gaacagcagc agcgccggag
     3961 gcttcaggct cgccagtccg atatggctca tcgcatggcg ctccaggatg tccaccagtt
     4021 gctcgaagat ctcggcactg ccagattcct gagactcggc ccgctcgcgc agcaccgtaa
     4081 ggctcagcac ccggcgttca gcgccgagat actcgatgat cgcagccgca tcgtaacgct
     4141 ccaagtcgtc catgcgcagc agcacgttct cctgcgtcag cggaagatgg cgcagcgact
     4201 ggacgaaatt ctccaggccg gtcatcttgg cgtgcagcaa ccattgcaga aaagaggccc
     4261 cgcggctttc caccgccacc gccttgagcc ggtagaaaat ggtgaacacc agcacgaaca
     4321 tgagcgacag cggaaagaac tcgaagaact cgcgcaccgt gagattgtcg gtgatcgcca
     4381 tgaaagacag cgccatgatc gaggcgcgca ggaaaatatt gagcacctcg ttgattacga
     4441 tgatgcgcgt gtccacgcgg taactcatgc gcaccagcac gtaaacgaag atcagcgcgc
     4501 cggcagcgcc cagacgccgc ggaatccagg tcagctcttc gcggaaatcg gtgatcacca
     4561 ggggcactgc aaacatcgcc gacacgaaaa tcgcattgtt caggcggatc tccagggtag
     4621 tgagcttggt ctggtcggca cgccacacca tccatgccat ggcgatgatg attgacacct
     4681 cgaaacacag aaaggcgatt accaggatct tgccggaatg cagttgcccg agcagcgtca
     4741 tgaggacgaa aaacacggtg ccgcccgcgg cgtagatttt catcggcagg ctgacgtgcc
     4801 ggcgcacgat gctctcggcg aacagcacga ttgccagcgg caatacggcc gtggggaaga
     4861 agcgcaggta atcgagccag gggtagcgga gcagccagta gaagccgcgc agtgtcatga
     4921 gcgcgaacgc gcagtagagc agaaacagcg tattgcgctc gagcgcgccg ccggtgcggt
     4981 ggtggtggac gaaattgagg tagccccata cacccgccgc gccgagccag gtgatgatga
     5041 tgtcgctgta aaccgcttcg ctgtactcca tgttgcctcc tctgttcgta cctttagcgc
     5101 cggggacgca ggtctctcca ctgccggttg ggcgagaacc agtgccacag cggcagcagc
     5161 agccttccgg ccagcacgaa catcacgcga tacagcaggc tgggcagcga gtcgcgcggc
     5221 accgtgcgca gcagatagtc gcaggcacac gcgaagttga tcgcgccgac ctgcaggaag
     5281 tcgtcgccgc gccggtagtc gagacggtcg cggcacatct cgttgaccac gtcgtcgcga
     5341 tgcttggtgg tctcgtcgta ggctttcttg aggctgtccg agccgccggt gtaggcgtcg
     5401 cggatgatga agttgtcctg gtcgaactgc gcctgcaacc ccacgccgaa aacttcgcga
     5461 tgcgactcca tcacctgccg ggcgatggtc aggtgcgcaa agcttctgcg cgtcttgccg
     5521 gagggcgacg ggtagacgtc ggcgaaggcc tccgcgacct tgccgacgat cgccggtacc
     5581 tgggtcacgc ttgtgatcca caccgggccc ataccgctcc tcaggaaaac cagcagacag
     5641 ggcagcccga cgaccaccca ggtgtaaccc tgcgcctgga acgacgggtc caccatgatc
     5701 agcccgagat gcaggacgtt aacgcggcgc ccgtgaactt cgacgtcgag aaaggagtag
     5761 gcgttgaagg cgatgggctt gcggctcgcc tggtcgtaga gaatcgagag gacggcgttg
     5821 tcaagccgcc gcttgtcgcc gctgagcacg ccgtattcga ggccggaggg aatcaccgtc
     5881 ttcacgacct ggcgcacgtc ggcgagaaga tggtcaagcg ccccacgccc catccacaac
     5941 ccggggcgct ccacgatgcg caggcgcacc tcgttcttca acggaaattc gaaatcgaga
     6001 tacttccggc ccagcttgtg caggtggatc atggtccgcc ctccccaggc ggtactcact
     6061 tcccgctacg aatttaccgc ttgtcaatcc ttcttcagac cacgcaacag aagctcgacc
     6121 aggagcgaag agatgcggtc caggctgcgc cattctatcc aggggtccct gcccttcgcg
     6181 atgtgcagcg acaccacccc gtgcataccc gaccacaagc tttgcaccag caattccgcg
     6241 tcgtcgagtt ccgggcgcag caggcctttt cgcatggcct cctcgatcgc cgccttgaag
     6301 aaggtataga cgtcctgcgc cggatcggcc tggatcgcgg cgagattggc ctccttcatt
     6361 tccgaggacg gcggagacat gaacatgagc cggtaatgat tcggatgctg cacgccgaaa
     6421 tccacgaaca cccgggccat cgccaggatg cgcgcgatcg gatcggcaat ctgggcgcaa
     6481 cggcggaatt cctgtccgaa ggactggaag tcgtgcgcga caatctcccg gatcagcgtc
     6541 tccttatcgg ggaagtgctg gtagatcatc gtggtcgtgt actcgatgcg ctcggcgatc
     6601 ttgcgcatcg tcaccgcttc gtagccatga accgcgaaaa gctcgcgtgc ggcatcgagc
     6661 acccgctcgc gcatgcgttg tttgtcgcgc tcgcgccgca gcctcgatcg cgacttcctg
     6721 gttaccggtg cgctgccgtg gcttccgtcc tcaagcatct cgaatcgctc ctcgtgactg
     6781 catagcatac cggctcgcgg cgatgctagc ggcccccatt agagtggtcg taccggtcaa
     6841 tggccgcccg ccagcaacac cagcgaaatg ccgaaggcat cgaagctctc gccggcgaat
     6901 gaatagcgct gatagtccag caggtgcagc ctgaccttgt ccgagccgaa caccaggccc
     6961 aggccgagcg ccagccccgt ctcgccgctg cggtcgcttc tgaccaccac ggtgcccgcg
     7021 gcgctgtcgt actcggctat cttgtagttg gcgttaacga gcccggcctt cagttgcagc
     7081 catcgcaatg cttcatgcct ggggcgcgcg gtgaggtaca gggcgttgga gctgaaggcc
     7141 atgcccccct ggtcgtggtc gcccaacctg ttttcggagg tccagccctt gaaccactgc
     7201 cactgtacgc cgaggctgaa gcgttccgtc tccttgaatt cgtaaccgac ctggatatcc
     7261 catccgcctt cgagtccatg ctgctggctg accagcccga atcccgctcc gatgtccacg
     7321 ccctcggcca gcaccggacc ttgaaccagc gcatagcata acgaggcagc gatcgctgat
     7381 tgagcgatcc gactccccca tgcgcgaaga gcgcgcgggc agctacatct gcccgcatgg
     7441 cggtagcgaa gcgcgtaatg gatagcgtcg tgagcggaac gcgctatgcg cttgcgattc
     7501 acgccctgga aatcgacagg tccgacgact tcgacgcggg ccacaagggc gggtacgcca
     7561 gcaaggcgcc agaaaccggc ggcgaaactg cgttcccagg gaaagtcggc ttccgggcag
     7621 agcgttccgt cggcgcgcgt caagctcaga gcgaaaaagt aaagcggaac accttcgctt
     7681 cccgcttcca gcagcgccga gttgaatgga cgaggttccg ccgccgcaca gatggtggcc
     7741 tcggggaaca gcgcgacctg ctcgccggca tcgaaggcgt cgtgcattgc ggccagcgcg
     7801 ctcaggacgc cgtcacgttt gccgcgatcg atgaatatcg tgccggcctc ggcgagcagc
     7861 catctcagcc acgcactgtt acccagttcc gacttggcca cgaagcaggc cggctggatg
     7921 gcgcaaatca ggatcggatc gatcgaggaa acgtgattgg cgacgagcag cgccggcccc
     7981 gtgccgaaac ccgggctcct gccgcaaatc agcgtgacgc cgagcgcccg caataccctg
     8041 cccgcccacc agcgcgtgaa gcgctcgcgc cgccgcaccg atgcaaacgc ggtgaccacc
     8101 gccgtgaaca gcgcgaacag caggaacaac gcggcgatac gcagcatgcg ccatgcacgc
     8161 accggcgcgg gcagcggacg ctgggcgcag gcgccaccac cacggccgcc gaccatgagt
     8221 tttgttgccg cgccgctcat gtatctccct ccgttcccgc ggcgtagcga tgcagccgcg
     8281 ggcccagcgt ttcgaggtcc aggacggtcg ggaaatcgat gcttccgaac tcgcgatcga
     8341 tcgcgggtgc accgcagatg cgcgcaccca gggacaggta cgcggacaac agtttgggcg
     8401 gatcgacgga aacacccgcc gacgtttcga gaggacaggc atagccggat cgcggccggg
     8461 tacggaaacg ctcgggcgcg aggtactgcc aggcgagatc gcgatacagt gccgcgccca
     8521 ccgcggcatc gacggtatgc agggagctgc agccgatcag atagcgcagc ccgtggctcg
     8581 ccgcgtattc ggcaatgccg cgccacaaca gtccaagcgc ggtcgggttg cggtgccgtc
     8641 gctgtatgca cgcgcggccg agttccccca gttcgggcag cagcggcccg aacgccgtga
     8701 gatcgaactc ggtcgccgag tacagactcg cgactgtcac aacgcgccgc ccgggcagca
     8761 gccggtaggt gctgacgatc gtcccgctgt cgcagtgctc gagcagaagg tgatcgcagc
     8821 gcggatcgaa ctcgtcttcg tcgagaccgg cgccgtaagc gcgcgccagg cccaccccga
     8881 gttcgagatt gaaaacctcg aaacgcagcc gctgcgcctg ccgcacgccg tgcgcatctt
     8941 cggccagcac gatgcgatat gcggcccgga cggaggcggt accgaggatg cgtgtgccga
     9001 tcgaaccttc ccgtgatctc acgatttgtc cctcaggcga tcatttgcga aatcatgaaa
     9061 atcgcgtaac cgagcgacgc cccggtgggg acgtccatca cgtaatgctg cttggtgaac
     9121 agacagctca gcccgatcag caccggaaac gccagaacca actcgccgaa caatggatac
     9181 aggtgcagcg ccgtcagtgt tgcgaccgaa gtgtgcatgc tgggaaaact gttcgagcgc
     9241 atgtccgcct tctgcaccag ggcaagcaat ttctccgacc aggtgcggcg caggttgcgc
     9301 ttgcgccagt cgcgtggcgt ggcaaccgga aacgccagga agaacagcat ttgcgccgcc
     9361 agcagcaatc cataactgga cgcgagcagc aggaacttgc gctccgagtc cacgatgaag
     9421 aacaccgcca cgatcacggc gtaatagagg atgctgtaga tccacaccca ttccggccgg
     9481 tacgggatca gttcgtcgat cgccgggcga aatacccgtg gcggcagcaa cggatagcgc
     9541 tggcaccaga agtagacctg gtgccctgcc acgaacagca ccactccaag cgcaaagtag
     9601 atgaggtact cttcggtagt catggccgcc ttccgggtcg attggccgca atcaggacag
     9661 ctcgatcatg tcgaccgcca gcgagtacgc ttcgcccctg gcgaatccga cggcgccgag
     9721 aaactcgtac gcatcgcaca cgctgtcgtc ggggtccatc tcgaagcccg aatcggcccc
     9781 gagcacatcg aagcgattgt cctgcgtcgc aatctgattc actgccacgg tcgtttccat
     9841 cggtctctcc tccttgtgtg ggttgcggtc gaacggttac ggcttcagcc gcggaacatt
     9901 gcgggaagcc aggtagtaag tgccggcaga taggtaatgg cgatgacgcc cattgccatg
     9961 accaggaagt acgcaagcac tgcgcggcac acttcggcga caggtttgcc gaagcggtaa
    10021 gaagcgaaga acaggttcat tcccaccggc ggcgtcagga atccgagttc catgttggcc
    10081 aggaagatca cgccgagatg cagcgggtca atgccgaagg ccagtcccac ggggatcagc
    10141 agaggcgcca ccaccacgat ggcggagaac acgtccatca ggcagccgac caccagcagg
    10201 aagccgttga gcgccagcag gaacacccag cgcgattcga tcgtgccggt ggcccactcg
    10261 accatccgat ctggcaactg cgcgtccacc agataattgg tgaagccaag cgcgactccg
    10321 aggatcagca gcacgccacc caccagcagc ccgcactcgg ccaccacccg cggaacatcg
    10381 cgcacgatgt gcagttcgcg gcgtaccagt gccgctgtca tgaacgcgta ggtcgcggtc
    10441 atggcggcgg cttcgaccgg cgtggcgaag ccgccaaaca gcgcgacgaa cgcgaccacc
    10501 ggcagcagaa gttcccactt cgcctcgctg atcgcgcgcc aggcctgcac gggatcgaag
    10561 cgcgcggcgg catttctgac ttcgtggcgc gccgcgtaga cggcgagaat gacggccatc
    10621 aacagtgccg gcagcagcgc agcaagaaac atgtcttcca taggtacgcg cgcgaccacg
    10681 gcatacagga tcagcggcag gctcggaggc agcagcacgc cgagcgatcc ggcactggtg
    10741 atgatcccga gcgccctgcc ttcgtcttgt cgcgtcgcca gcagcaatgg catcagcaac
    10801 ccgccgagcg cgagaatggt cacgccggag gcgccagtaa acgcggtgaa cagtgtgctg
    10861 gtcgcgacca cgacgatcac cgaaccgccg ggcaatccac ccaccaacgc cttgaacacc
    10921 gccaccagcc gcgccggtga gccgccctcg gcaaggaaat atccggcgag cgtgaacaac
    10981 ggaatcgcgg gtagcgacgg attcaccgtg agccggtaga gatcgatcgg gatcgaggcg
    11041 atcggcagtc cgtcgctcca gaacaggatc agcgccgcgc cggccagcgt ggtgaatacc
    11101 ggtgcaccga ggatggtcgc cacgagcagg ccgatcagcg cgggaaaacg caacgcttcc
    11161 gcggacagcg gcccgtaagc ggccagcacc gcggcacaca gcgcaacggc ggcgcacgcg
    11221 gcacggatcc gccagccctc gcccgaatgc aggaccaggc gaaccgcgat cagcgcaaac
    11281 ccgggcggca tgatcgcctg caccacccac accggcaggc cttcgaccag cagattgccc
    11341 gcgagttttt cggtgatgac caactgcgtg ctcgccaccg cgagcagggc cgacaccagt
    11401 gcgccaacgc agccgctcag ggcgtgtgcg gccgaccgtg tccagccgcg cagaatcaac
    11461 ggcaggctgg acaaggaaag cagccggttc tcgcgcgccg cgatcgccgc gcccagcatg
    11521 cccgcgatca ggaccaggtg ctgcgtcagc gtcgccgcgc ccggaatgcc gacgtggaaa
    11581 accgcgcgca acaggatctc ggcaaccggc agcagcgcca atgccgccag tgcgaacgcg
    11641 accagcaggt tctcgccggc gacgagcgcg cccttcgcgt tcatcgccgg aatcacgccg
    11701 gcgggtttgg catcgagcgt catcgcgata ctcatttgcg cgccagcccg gccgccctga
    11761 actcggccac caggcgcagg gcctcatcga aaatcggggc ggggaccgcg gtgccgcgga
    11821 tgcgcggata aacttcttcc gcggtccggc gccactcggc ttcgatctcg ggagtcatgg
    11881 tcgccacctt cagtccccgc cgcttcatcg cctcgatcga ctcgaggtcc tctgcgcgtc
    11941 cccggctgcg tatctcggcg ccggcctgcg cggccgcgcg tttgagttcc tcgcgcgccg
    12001 tctccgacat cgcgtcccag gccttcctgg tcacgacgca tgcgccgacg atcggcaccc
    12061 acttgacgtc cagcagatag ggcgccgggc cgttgaactg gccggcaagc gcgaaaaatg
    12121 gcgcggccgg cacggcgtcg atcagacccg tctgcagccc cggaagaatg tcggcggttt
    12181 cgatcggcac cggctggtag ttcatacttt tcatgatttc cacctgctgc gggtcgccgc
    12241 tccagacgaa catcttcatg cgccggaaat ccgccgggcg cagcgccggt gcgttggaga
    12301 agtagcgcac ccagccgccg tctccccaga acagcacgac gaagcccttc tccagcaggc
    12361 gcgcttccag cgtcggcccc agccgctcgc gcacgtagtc cacctcgtcc cagttgcggt
    12421 aaaccatcgg catgtactgc agcgccatca ccgaacggtc gatctccagc aggccgatca
    12481 ccgacagcag cccggcgttg agctggccga cgcgcatgcg ccgcaccacg tccgcctcgc
    12541 cgccctgagt gccgtcggcg aaaatggtga aggtggcccc agccccttgc gccttgcgcc
    12601 acctctcccc catttcctgc aacgcgcggt gataactcga acccttcggc accagcgtgc
    12661 cgaggcggat gcgcatggcg tccgcggcct gcgcctggtg ccgcaacgtc agcgcaggaa
    12721 gcgcagttgc aaccccggcc gcgaaacaca ggcgccggcg ttgcgtatcg atacgggcaa
    12781 tgtcgttcat gacggctccc tcattcggca aagagttggt cggattgcgc caggagccag
    12841 cgcgcgcgcc gctgcatcac ggtgttggcc aggcgccact cgggccgctg gccgggcttg
    12901 atcgcgagcg cctgcttgag cagcgcttcg aattcactgc ggtcctgtct gggcacggca
    12961 accgactccg ccagcgacac atagggtcca gcctgcaagc cgccggtgag actcactgcg
    13021 cggtcaaagt gatggcgagc gcgggtggcc gggtcgcccg tggctccctg gcgcaccatt
    13081 tcgaaagtga tgaggaagtt gtggattgct ccggagtcgt aggcctcgtc gagcacagcc
    13141 gcgcgatcga tgagcgcttc caccaccggt acgtcggcga ccagatcggg gtcgtgcttc
    13201 gacagcgaga tcaatgccgc ccacgacact gccgtccagt acagcaaggg cacgtcgtcc
    13261 ttacgcgcaa tgcgcgccgc ggattttgga tccaggcgaa gcttgcggct gaattcggga
    13321 tgggctacat cgaggccgcg caacccgtaa tcgcgggcgc ggcgatacat tcgttgggcg
    13381 cggctgcgtt gctccgaggc ggccttcagg tcttggtctt cgagccggtc cgcgtcctgg
    13441 tggacaaatg cgtaagcgta tcgggtaaag ccgctgctgg tggcgtaaag caggccacga
    13501 tggcgcggac tttccgccag caggctttcc atgagtttca ggctgaacgg cgcagcgtcg
    13561 cgtatcagct ccggatcgtc gtcggccgca aacacgatgc cgcttgaagc cagagcatcg
    13621 ccggccttgt tgatggccaa ctgtcgcacc gagcagccgg gcagaaggcc aatcatgaga
    13681 cccagaagca ggaccagccg ggtccagcca cccccggaaa aaacccactc cccgaaccaa
    13741 ttgctgagcg atgccagcat gtccccaccc tcctccgagt ttctcgatat gttcttcaca
    13801 tcaattagtt aactaatcat catgatccat gttctttctt attacgatgt tattatacat
    13861 aacaaggtta ttttgtcaat aaaaaatagg ggcgccgtcg tcctgcgccc cacgttgtta
    13921 cgcaactcta tgatctgacg agattctata tttccttccg aaccggcccc gtcatgccgg
    13981 cgatgcgggc actaactggc cttatcgcct tcgggccagt ccttgatgta ctgcttgagc
    14041 agcttgtttt cgaagttgac cgcgcgcagc gccgccttcg caacatcgtg gaacgacaac
    14101 acgccgatca gcgcgccgtt ctccatgatc ggcaggtaac ggatatgttg gtcggtcatg
    14161 atggtgcgca tgtggtccgc gctctcgtcg ggattggcgc agatcggctc catgaccatt
    14221 acctcctcga cccggacttc gtggatatcg ccgcgatggc ggttcacggc ggcgagcacc
    14281 tcgcgaaacg tgagcagtcc ggtcattttt cccttttcca tcaccagcag cgagccgatg
    14341 tcgcgttcga ccatcagccc gaccgcttgc ggcagcagcg cgtccggggc gatgctgaag
    14401 atctggccac ccttgatcct gagtatgtcc ttgaccagca tcatgcccgt cctccttgtc
    14461 ccggccccat tgtcatcgga ccgcttgcta ctctaaatca cgccgcggga tttcaagtca
    14521 gcgattctcg ccgcgtcgta accgaggtgg gcaagcactt caccggtgtg ctcccccagg
    14581 gccggaccga gccagcgcgt acctcccggc gtctgcgcca acttcggcac cacgccgggc
    14641 actttcatcg gctcgccgtc cgggagccgt gcctgttcga tcatgccgcg cgcctggtaa
    14701 tgcaggtcct tcacgatgtc ggcgatgtcg tagattttcc ccgacggtac ctgcgcggca
    14761 gccagcacct ccagaacgtg accgaggtcg tgcgccgcgc accagtcgcc gatcacctcg
    14821 tccagttccg cggttcgtgc cacccgcccg tcgttgcggg cgagagacgg atcattgccg
    14881 agatcctcgc gaccgatcgc cgtcatcatg cgcctgaaaa tcgcatcgcc attcgcgccg
    14941 atgacgacat acttgccgtc gcgggtggta taggtattag aaggcacgat gcccggaagc
    15001 gaggcgccgc tgcgctccct gacgaaaccc gtcatatcga actccgggag cagactctcc
    15061 atcaggctga atactgcttc gtacagggcg acgtctacca cttgcccgcg gccgccgttg
    15121 acttcgcgat ggcgcaacgc catcagcgcg ccgatcacac cgtagagcga cgcgaccgca
    15181 tcgcccaggc tgatgcccac gcgcaccggc gcccggtccg gatagccggt gacatagcgc
    15241 agaccaccca tcgcttcacc gatcgcgccg aagcccgcac gctcgcggta cgggccggtc
    15301 tggccataac cggacaggcg caccatcacc aggcccggat tcaactgcgc cagcctgtcc
    15361 caccccagcc cccatttctc cagcgcgccc ggacggaagt tctcaatcac gatgtcggct
    15421 ccggccgcga gcttgcgcac gatttcctgg ccctccggtt ccctcatatt gacggtgacg
    15481 gatttcttgt tgcgcgcctg caggtaccac cacaacgatg tgccgttgtg gagcttgcgc
    15541 cactggcgca gaggatcgcc gccgtcgggc gactcgatct tgatcacctg cgcgccgaat
    15601 tcggcgaaaa tgcgcgaaca gaacggcccg gcgatcaatg tgccgagttc cagtaccttg
    15661 atgtcggata gagggcccat gctgcggtga aggcgtgaag gtgtgaaggc gtgaagggta
    15721 aaagcgcagc gcttggcgct tggagtgccg gacgcgctat tatctgcaaa aatccgcaaa
    15781 cacgggaatc gacacgatga aacgttctcc tgctctgctc gccgccctgc ttttcctgac
    15841 cctgatcagc gaggtttcca tggccgcatg tcccgatctg ctcaaccatc agatgaataa
    15901 tctcgacggt aaaccggtga atctgtgcga ttacgccgga aaagtcgtgc tcgcggtgaa
    15961 caccgccagc tactgcggca acacgccgca atacaagggg ctggaatcgc tttaccagaa
    16021 ataccgggac aagggcttgg tggtggtcgg gttcccggcc aacgacttcg gctcacagga
    16081 gccgggcacc gcgaaggaaa tcaaggattt ctgcgagctg acctacgggg tgaagtttcc
    16141 gctgatggag aagtccagcg tggttcccgg caaggccaac ccggtgttcc aggaactcac
    16201 ccggatgacc ggcgacgctc cggaatggaa tttccacaag tatctgatcg cgcgcgacgg
    16261 caagcgcgcc ttcagcttcg ccgcgcggac ccagcccgag accggcgaga tcgtcaaaca
    16321 gatcgaagct ctgctcgccg aaaaacctta gaaattcttt tcaccgcaaa gacgcaaagg
    16381 tacgcaagga aaaacaagaa acagagagac agatatttct gcacgaggtc ggcgtcaagt
    16441 gtcgaccgtt ttttcttcgg gataccatcg cgaaagtggg ctggatgtcg tctttcggcg
    16501 ggtccccaat ccagttgaac tggatttctt gagttacagg atttccttcg cgtgcctttg
    16561 cgtctttgcg gtgaaggtgt agatccgcta cgcggaaaag tcgcgaagca cggagccggc
    16621 gagaggcgcg tccttgagga tgccgttcga gtcggcgacg agttcgccgt ttacccacac
    16681 gccgtgcacg ccgatgccgg gcgtgtgaat ccgcgatcca cccgcgggca ggtcgtagac
    16741 gcgctgggac ggagcccggc ccacggtgtg aggattgaac agcagcagat ccgcccagtt
    16801 gcccgccgtg atcgcagcgc gtccgcggat gccgagcaac tgcgccggtt ccgaggtgag
    16861 cttcttcact gcaatttcaa gcggcatgag tttgcgttcc cggacccaat ggccgagcag
    16921 atgcagtcca aagcccgcat cgcacaggaa agtcaggtgc gcgccagcgt cggacagcga
    16981 cacgatcgcg ttcgggtcgt tgagcatcct gcccacggcg tcctcgtttg aattgaggag
    17041 cgtggcggtg aacagcgtat cgagatcttc ggaaagcgcg agatcgagca tgaagtccag
    17101 cggatgcgca ccctgctttc tcgccagggc gccgatcgat tgcccctcgt agtccgcatg
    17161 cgccgggtct gccacctttg ccacgaaggc cttttcccat tcgccggtga acatgcgccg
    17221 cgcgcgcttc gacaattcgt ccttgaagcg aatgcggaac gccggatcgc cgagcgcctt
    17281 cttgacctcc agctcttcca tcttcatgag cggcttccac gcggccagcc cctcgaacac
    17341 gtacggctcg tgcatggtga actcgaagtt gagcgggcac gcggagaccg cgccgtacat
    17401 gcgccggccg cggccgcgtg cccggctgat gtgttccagg tcctcgaacg acgcttcggg
    17461 cgtgaggttg gagtgaagca gcgcggcgac gagataaggc ctgtccgccg ccgcagccag
    17521 cgactcgatc cagggaaccg gtgtatccga cgccttggtc atcatcagga ggcctttccc
    17581 accttcgcga agcgcaccgg acagggccag aaattcgtgc tcgtccgcga ggcgcgaggg
    17641 catcgggatc ccgttttcgc cgttgtgctg gtacgagcgc gtcgtggaga agccacaggc
    17701 gccggcttcc atcgcctcga ttacgatgcg gcgcatctgc gcaatttcgt ccggcgacgc
    17761 ggcgcgcttc gcggcgtcgc cgcgcaacac gaaagtgcgc acgccggaat ggcccacgaa
    17821 gcaggcgacg ttcggcccga ccccattctt ttcgagcatc ccgacatatt ccgggaacga
    17881 ttcgaacccc cagtcgatgc cggcgcgcag cgcctccagc gacatgccct cgacgtgggt
    17941 gaggttgcgc atcgtgaggt cccgatccgg cggccggcac ggcgcaattg tgaaaccgca
    18001 gttgcccatg actaccgtgg tgacgccgag tgcgggagag ggattggcaa acgggtccca
    18061 ggtgatctgc gcgtcataat gggtgtgggt atcgatgatt cccggcatca gcgccagccc
    18121 gtcggcattg acgcgttcgc gtgcctcgcc ttcgaccttg ccgacttcgg caatccgtcc
    18181 gtctgttacc gcaacgctgc cgcgccgcgg cgcgctgccc aggccgtcgt atatcgtcgc
    18241 gttctcaatg atcagatcgt gcattttttg ccccgtctat ttaaaacctg aaaaggcatt
    18301 tgccacagag gtcacagaga acacagagga aatgcaaaaa cgagaataac aaatgtgcgc
    18361 caaaagaggc actgttcacg aacctgcatc gctaattttc accacgacag atcatgagca
    18421 aacgaacggt tttaacccct gtttcttttt tcttcttgat tctcctctgt gttctctgtg
    18481 tcctctgtgg ctaattatcg tcttttcgag atcaccggta gtagcgcttg gcgcggctct
    18541 ccgcctgttc catagcggat ttgatagtgg aagcaccgga tgccgcttgc gcaaacatgt
    18601 tgaccacgat gtagtcggcc atggcacccg ccgacgccga gccgagcttt ccggcatagc
    18661 cattgtcgcg cgtcagcccc ggcacgttgc ggaacggcaa cagtttcgga tcgctcttcc
    18721 acaccggcgt atcggcgtag gccttgagcg gttgctggac ataacccagc gaggccgact
    18781 gccaggcctc gtactggctg cgttccatca tgaaacgcag gtactccttc gccgcgttgg
    18841 gatacttcgt atatttgaat acgaacatcg ggctgaaggg attgagctct gcggcatggc
    18901 cgaccggtcc gatcggcatg ttcacgtgct ggatgtcggc aaggagcttc tgcacgccgg
    18961 gttccttcga cgtcttgacc gcgtaataga tcgacacccc gttcagagtc aggctgatgt
    19021 cgccggacag gaacgccttg ttgttgtttg gatccagcca cgacagcgtg ccgggaatga
    19081 aggtctggta cagcgccgcg gcatactcca gcgcggcggc cgtttccttc gaattgatga
    19141 cgacgcgatt gctctcgtcc accatcctgc cgccgtgggc ccacagcgcc caatgggtcc
    19201 aggcgttggc atcgcccacg gcgttgccga gcgcaaaacc gcacggcgtt cccttcttct
    19261 tcagcgcctg gcacagcctg aggaatccct gcgtatcgtt cggcaccttg tcaaagcctg
    19321 ccgccttgac cgcgctttcg cggtaaacca ggcagccgcc gagaaatccc gacccgatgg
    19381 cgatccactt gccgtcgtgg atgcaaaagc gcctgaccac gtcgtaccat ccgccgtact
    19441 tcgcgtcgag ataattggcc agttcggtga gatcgagcag tttgtccgcg tactggtgcg
    19501 gatcgtcgta ccagccgagc acgatatcgg gcccgcttcc aacgttggcc gccaccgccg
    19561 ctttcggccg gatgtcctcg aagttctcgg aatccacccg caccttcaca ccggtggcct
    19621 tgtagaactt ttcgctgttg gcgagccatt gttcctcgtc gccctggacg aagcgcttcc
    19681 agcgcaacac ccgcagttcc gcgccttttt ccggctggta gctcagatcg gcggcgtgcg
    19741 cgcgatgcgg cccgatcaat gcgcttgcgg cgcccgaaat gaccgccgcg cccatcgccc
    19801 caagcgcttt gcgacgcgag aattcgctca ttttcccctc ctcgtcagag ccgccactgt
    19861 tcggacggct gccgcggtcg agcccagacg gaatgcgcag gttgacggcc ccggcttgca
    19921 ggtccggggt acaacgcgca tcggctcggt cgcgctgact cgcgaccgag cctaccttcg
    19981 aaccaggccg acaaacgtgc gactacgtcc gcggatagtg aatttccagc acgttgagcg
    20041 tggtggagcc ggcggggcgg ttccaggtaa cggtatcacc cacttttgca cccatcagcg
    20101 ccttcgccag cggcgactgc cagctgacgc ggccgtgggc tgcgtcggtc tcgtcctcgc
    20161 cgacaatcgc tatggaatgc actgcgccgc cctcgtcctc ggtcttgacg ctggcgccga
    20221 agtgcacttc gtccctgggc tgcttggaaa catcgaccag gatcgctgat tccagccgcg
    20281 cctggtagta gcgcaggtcg cgctcgacct gcgcgagcct gggccggtcg aaatcttcct
    20341 gcgccccctt cagacgcaca tgttcgtcgt gcagcttttc gacttttctg cgcagtttcg
    20401 cgagtcccgc cggcgtaatg tagttcggct cggaattgac cgggcgctcc ggcaattctt
    20461 cgctgggcag ggcttcgttg tcgtctttta cgaaggcgcg gctcatcttt tgcctctcgt
    20521 ggaatcgtac gactgggcgc caggccatgc ccggcgtgag ggaactttgt cagagcttcc
    20581 ttaaggcaga tggtaccaag tatcgcacgg gcggcgttcg ctgtattggg ctggccgcgg
    20641 gcctatggca ggaggcggaa acgccgctcg atcagatcga cgtcgtcgaa gtggcggcgc
    20701 ccgacgaaag ccgtggcatg gtgtccccac tggcgcatgg cgccggcgct cggcaccccc
    20761 atgggccaga aaaagacgcg ctcaccgcgt tcggtacccg gcaccagggc gtccggccgg
    20821 aaggcgctgc ggcgttttcc agcgccggca ggcatagagc gcagttcgtc gtaatcggcg
    20881 aattcgtagg tcaggccagc gccgccgcca tcggctgcca tgttcaccag gtaatgggtt
    20941 cgagttgcca agcgcaacac caagcgcgca ccgtccggaa gtttcggtgc ccggtccggt
    21001 acgaaggccc actcgatgcc tgttgccggc ggcggcaacg gttccactcc cggaaccggg
    21061 aagaacatat ggaagcagcc acagggatgg atagtgtcgt aaaccagcgg atcaccgtgc
    21121 gtggacagcg tgattcgcca gaagatgcca tcgagccggc ccgccagcag gtcgagcgac
    21181 ttcgcgcgcg ggcgctcgct gaaccaggcc agatagacaa gctgcaccag cgtttcacca
    21241 ccgtagcggg taagcgcgag ctttcggtag accaccggcc gcgaagtatc cacgaccggc
    21301 cgcttgccct cgccccacgc aagcgcgccg atgcgatcgt agccgccgcc ggtttcgacc
    21361 tcgatgaccg gcgcaaacgc ggcgaacagc gcctcctgtt gctcggcgga aagttgcggg
    21421 atccccaacg gatcgcggcg cgcgcccgca aggatctcct tcgccgcttg cgtgccggcc
    21481 gcgtccggcg gcgcgtaacg cgaaagcgtc gccggcccgc gcatgtcttc cttcgagcgg
    21541 cgcatcgtct cggcagcttc cttttgccag ccatcgacgc cgtaccagaa cggaatcctg
    21601 gtgatggcat acaaacccaa cgccctctgc caggttacat agtcgtccgg cactcgcgcg
    21661 cgcgcggcca gcagcgtgcg cctttccggc acggcaaagt cgtgccctgc cagggttgtc
    21721 gcgcaagcct gcgtcctttc cccggcttgt gcgcggttcg cagccaggtc ctcgagcgaa
    21781 ccagccggca gattgctcag ttccgctgca cgtgcctcgg cctcaaggcg aagcaatcgc
    21841 cccgtccatg cctggaacaa atcttcgccc gcaagcgctt cgatgcggaa tgaagcgccg
    21901 aaacgatcgg tgcgcaaata gggaaaaccg gggatgcgat attcgccgcc atcgcgcacg
    21961 ccggcctggt cgatgcgctg atccatggcg cggtaccacg cggcacactg cgcaacggct
    22021 tcgtttgcgt ccgacaggtt cgcacggatc ggattctgta acgtcgcaca gcccgtaacc
    22081 aacgccagca cgaaaacggc aaccaggcag gttctgccgc gccgcgcagc cacccggtcc
    22141 cgcgttcggc ttcgaattcg catcatgccg cacatcgtaa cggccgcagg cgcgcgtgta
    22201 caatcgcccg gacgatgcgt cttccgcgaa gcggctggtg atatcgcatc cgggcgctgc
    22261 atccggacgt atatcgcgca cgcgcgggtt tcagcatcac cggcgcgtcg tatcaggttc
    22321 ggcgcgctca cccggccaag cgcagggcat gcgggaacaa caaagggggt cttgcggtga
    22381 ccaaaggaaa attgctgttg atggttttga tcgccgcgct gattgcagcg ttcatcgttt
    22441 tcgacctcgg ccaatacttc agcctgtctt acctcaagtc gcagcaggca gctatcgaag
    22501 aattgtatcg cgccaagccg ctgcagaccg tggcggcgtt cttccttata tatgtggcgg
    22561 cgaccggcct gtcattgccc ggcgcaacta tcctgacgct tgccgcgggg gccattttcg
    22621 gcctgctgtg gggcacggtg atcgtctcct tcgcctccag catcggcgcg acgctggcat
    22681 tcctcgtctc gcgcttcctc cttcgcgaca ccgttcaggc gaagttcggc gaaaagctga
    22741 aggcgatcga tgccggcgtc gacagggacg gcccgttcta tcttttcacc ttgcggctgg
    22801 tcccggcctt tccctttttc gtcatcaacc tggtgatggg gctcacttcc atcaagacct
    22861 ggaccttcta ctgggtaagc cagctgggca tgctcgcggg caccatcgtg tatgtgaatg
    22921 ccggcaccca ggtcgcgcag atcgaatcgg cggccgggat tctttcgccg gcgctgatcg
    22981 cctccttcac cctgctggga attttcccgc tcattgcgaa gaagatcgtt gccatgatca
    23041 aggcgcgcaa ggtgtactcg cgcttcaccc gtccgcgcaa attcgatcgt aacgtcatcg
    23101 tcatcggcgc cggctccgcc ggcctggttt cggcctacat cgccgccgcg gtgaaagcaa
    23161 aagtgacatt gatagagaaa gaccgcatgg gcggcgattg cctctatacc ggctgcgtgc
    23221 cctccaaggc gctgatccgc tctgcgaagt tgctgtcgca tatcaagcgc gcgcgtgaat
    23281 tcgggttgcg ggaggccacg gcggatttcc agttcgccga cgcaatggag cgggtgcagc
    23341 gggtaatcaa ggccatcgag ccgcacgatt ccgtggagcg ctacaccagc ctgggcgtgg
    23401 agtgcatcaa gggcgaggca aaaataatct caccgtggga agtgcaggtt aatggcaaga
    23461 cactcaccac acgctcgatc gtgatcgctg ccggcgcccg tcccttcgtg ccgccgattc
    23521 caggagtcga aaatgtcggg catttcacct cggacacggt ctggaacctg cgcaaactcc
    23581 ccgcgcgcct ggtggtactc ggaggcggcc cgatcgggtg cgaactggca caatgcttcg
    23641 cccgcctggg ttcgcaagtc acccaggtcg aaatgctgcc gcggatcatg attcgcgaag
    23701 accccgagtt ttccgaaatg gtcctggctc gcttccgcga agaaggcatc gacgtgcggc
    23761 tagagcacaa agccaaacag ttcctggtcg agaacgggga gaaggcgctc atctgcgagc
    23821 acaaaggccg ggaggtgcgc atccctttcg acgaagtgtt ggtggcggtg gggcgaatcg
    23881 cc
//
LOCUS       DASQOP010000211         2428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2311817, whole genome shotgun sequence.
ACCESSION   DASQOP010000211 DASQOP010000000
VERSION     DASQOP010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2311817"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..230)
                     /locus_tag="VE030_08610"
     tRNA            complement(155..230)
                     /locus_tag="VE030_08610"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(195..197),aa:Arg,seq:cct)
     gene            511..1683
                     /locus_tag="VE030_08615"
     CDS             511..1683
                     /locus_tag="VE030_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZF23512.1"
                     /translation="MKSRLPGTVIVLGFVSLFNDLASEMIVPLIPILLAGVLGAGPIA
                     LGLIEGVADAVASFLKLWSGRHSDRLGGRRKALTLAGYALSNIARPLLAFAGSWASVL
                     LLRSVDRIGKGLRSAPRDALVADATPADIKGYAYGFQRALDNSGAVGGSLVAAAVLAW
                     SGLPLAQVILLSAIPGFLAVALIAFGVREPPAAPAAVPRTLARLRWGALSPGMRRYLL
                     VLALFTFARASETFIVLLGYQKGAATVELLLLWAALNLAKALTSTAGGRLADSLGRGP
                     LLLLSWTAFAASFLLFGKVNSGIGLWMVTIAYGLFFGLGEGAEKALISDFATPAEQGT
                     AFGWYYLILGVAAIPAGLLFGAVWQFQSAAMAFFLAAAVAAVATLLLRVWAWPSRI"
     gene            1899..>2428
                     /locus_tag="VE030_08620"
     CDS             1899..>2428
                     /locus_tag="VE030_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZF23513.1"
                     /translation="MPIATFENVSLAYGHVALLDHADLVIEPGSRIGLIGRNGAGKSS
                     LLKILAGLAAPDDGRVWRRPGLRIAFVPQEPVLESGHTVFEATVAGLGELADLLASYH
                     SAARVLEHAPHDETAMAAMQSAHEELERRDGWNLQSRVETVLSRLGLSADARVEALSG
                     GTRKRVALARALVADP"
BASE COUNT          408 a          726 c          788 g          506 t
ORIGIN      
        1 acggtattga acttcatttt ttgccagtct cccaggcgca ctgacaggag cgaactgagg
       61 gaaaaggtgc ggcaccggga ggacgaaacg ggcaatgctg cggaagtcgc gaccatcgat
      121 ccacgcccgg aagcatccgg cgaagtcccg gtgatggcgc cccgaacacg aatcgaacgt
      181 gcgacctacc ccttaggagg gggttgctct atccactgag ctaccggggc gtaggggcga
      241 attctaaccg aagaaggccg caaaggccgt gaagcgagaa gacgtaaatg tcgtgaatat
      301 cgcaaggacc cgcaaaagcc cgtaagaccg tgaagcaaga tgacgtgaaa acgacgaatg
      361 gagtttcagt gaaggctaat gtctgcgtag ccgacgttat gccggagtta atcccgtaag
      421 gactcaaggg ctgtacggct gctgaattgg ttttgcgata ctcacgatat tcacggaatt
      481 cacggttttt cccggttacc attcgtggcc atgaaatccc gtctccccgg taccgtgatc
      541 gtcctcggct tcgtcagcct gttcaacgat ctcgcctccg aaatgatcgt gccgctgatc
      601 ccgatcctgc tcgccggcgt tctgggtgcc ggaccgatcg cattgggact gatcgagggc
      661 gttgccgatg cggtggcgag cttcctcaag ctctggtccg gccgtcactc cgaccgcctg
      721 ggaggacggc gcaaagcgtt gacgctggcg ggttacgcgt tgtcgaacat cgcccggcct
      781 ctgcttgcgt ttgccggttc atgggcgtcg gtgctgcttt tgcgcagcgt ggatcggatc
      841 ggcaagggac tgcgcagcgc gccgcgcgat gcgctggtgg ccgatgccac gcccgcggac
      901 atcaagggct atgcctacgg tttccagcgt gcgctcgaca attccggcgc ggtgggcggc
      961 agccttgttg ccgccgcggt actggcgtgg tccggattgc cgcttgcgca ggtgatcctg
     1021 ctttccgcga taccggggtt tctcgccgtg gctctgattg cgttcggcgt tcgcgaaccg
     1081 ccggctgcgc cggcagccgt gccgcgcacg ctggcgcggc tgcgctgggg tgcgctgtct
     1141 cccggtatgc gacgctacct gctggtgctg gcattgttca cgttcgcccg cgcctccgaa
     1201 acattcatcg ttctgctcgg ttaccagaag ggggccgcca cggttgaact gctgctgctc
     1261 tgggccgcgc tcaatctggc caaggcgctg acttcgaccg ctggtggcag gcttgccgac
     1321 agccttggaa gagggccgct gctgctgctc tcgtggaccg cgttcgcggc cagttttctg
     1381 ttgttcggca aagtgaattc cggcatcggg ttgtggatgg tgaccatcgc ctatggtttg
     1441 ttcttcggat tgggcgaagg cgcagagaag gcgctgatca gcgattttgc cacgcctgcg
     1501 gagcagggca cggcgttcgg ctggtactac ctgatactcg gcgttgccgc gatccctgcg
     1561 ggcctgctgt ttggcgccgt ttggcaattc cagagtgcgg caatggcgtt tttcctggcc
     1621 gcggccgttg ccgcagtcgc gacgctgttg ttgcgcgtct gggcctggcc ctcgaggatc
     1681 tagatcttca ccgcggaggc gcaaaggcgc agaggaaacg cagagaaaac caaacgaagc
     1741 gtgtcaaaat gggcaaggat ttcgatgcgc cgccacccaa tcgaaaaatt ggggtgtcgc
     1801 tactatccac ttcttatctt gccttttcac cacaagttat tttgctttcc tttgcgtttc
     1861 ctctgcgcct ttgcgcctct gcggttaatg tttagtcgat gccgatagca acattcgaaa
     1921 acgtctcgct cgcctatggc catgtggcgc tgctcgacca cgccgatctg gtgatcgagc
     1981 cgggcagccg catcggcctg atcggccgca acggtgcggg aaaatcgagc ctgctgaaaa
     2041 tcctcgccgg gctggccgcg cccgacgatg gccgcgtgtg gcggcgcccg gggctgcgca
     2101 tcgcgttcgt tccgcaggag ccggtgctcg aatccggcca caccgtgttc gaagccaccg
     2161 tcgcgggatt gggcgaactc gccgatctgc tcgcttcgta tcattcggcg gcgcgcgtgc
     2221 tcgaacatgc gccccacgat gaaacggcca tggccgccat gcaatcggcg cacgaggaac
     2281 tggagcgccg cgacggctgg aacctgcaaa gccgggtgga aacggtgttg tcgcggctgg
     2341 ggttatcggc ggatgcccgc gtcgaagcgc tttccggcgg cacccgcaaa cgcgttgcgc
     2401 tggcccgcgc gctggtcgcc gatccgga
//
LOCUS       DASQOP010000212         8964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_97259, whole genome shotgun sequence.
ACCESSION   DASQOP010000212 DASQOP010000000
VERSION     DASQOP010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8964
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97259"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..829
                     /locus_tag="VE030_08625"
     CDS             92..829
                     /locus_tag="VE030_08625"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZF23514.1"
                     /translation="MIPIRDTIPSRSAPVVTWALIAVNATVFLCELQFAPEQLEQFFY
                     LFGMVPARYSHPEWARAIGFPIDDYWPFLTSMFLHGGWLHLIANMWTLWLFGKAVENR
                     MGRMRFLLFYLLTGLAAGLTHWFTNVDSTMPAVGASGAIAGVLGAYFILYPRARIIVI
                     LPVFFLPFFFELPALIYIGFWFLSQVFGGTLSGLGPDDVGGVAWWAHVGGFLAGVVLY
                     RLFLLPPGRAPRKYEPDEYDIEQTWAR"
     gene            864..1706
                     /locus_tag="VE030_08630"
     CDS             864..1706
                     /locus_tag="VE030_08630"
                     /inference="COORDINATES: protein motif:HMM:NF014074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HZF23515.1"
                     /translation="MDIGQLIWLFFMLTALQPVIKQRLLESSRQRLIARIENRRRSRV
                     ILLVHRQETMSFLGFPLMRYIDINDSEDVMRACEMTDPEVPLDIVLHTPGGLVLASLQ
                     IARAIRRHKGKVTVFVPHYAMSGGTLIALAADEIVMSPHAVLGPVDPQLGQFPAASLL
                     KVVSRKPIAEVDDQTLILADVAEKAINQLRVSVKEILTRSQTPEKAEELADLLATGVW
                     THDYPITDEEARRFGLKVSTEIPADVFQLMGLYPQPLRRQPSVEYLPIPRRAQRTKPG
                     STTE"
     gene            1822..3795
                     /locus_tag="VE030_08635"
     CDS             1822..3795
                     /locus_tag="VE030_08635"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HZF23516.1"
                     /translation="MSAVVALAATGSNAAEPLLTAGLYQIEVRIALPNVQDVVAPMVL
                     NRCLSADDLKSGQAFFVRSDNPLRNCDQLDYQVTGGTASYRIACPGPNKGSAVAVFET
                     TAITYRGGIKMNMGGKNMTMSETQVAEKSATAASDPAIPQGIGPHSNVIMPIVRINVA
                     AMGLARFLRRCALPACCLLAAATWLVAAEAPRLDPAQLSGGILTVADESEAAYLQPAP
                     SLDGEALKLFAEGHKFFNTQWAFYWFEGGMWGRGPTSNADSCVACHRGNGRGSPDAPS
                     RDASAQRAHECSAPPQGPERDSSPQLLCRRIEASALSLILRLSLPGENAHGGPRPHPH
                     YGDQLQTFGVPRLLPAEGTVQVTWNERSVTLADGEVLQLRTPQIRITGLAFGPLGEDI
                     MISPRMAPPLIGMGLLEAIPDATIIALAEREPNDGIRGKTNVAWDMIAGKTSIGRFGL
                     KANQPTLRQQIAAAFIGDLGLSNDLFPEQNCPPIQKTCKEMMFAGNPEITVHRLTAVE
                     TYLRTLAVPARRNADDPQVVRGAQVFETARCAVCHVPQLQTGEFPAAPLLAHQTIRPY
                     TDLLLHDMGENLADHRPDYRAGGSEWRTPPLWGFGLSEKVNGHGALLHDGRARNTAEA
                     ILWHGGEAAVSRDAFRAMPKADRDALLAFLASL"
     gene            complement(4249..4635)
                     /locus_tag="VE030_08640"
     CDS             complement(4249..4635)
                     /locus_tag="VE030_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HZF23517.1"
                     /translation="MATKKKSARKSAKKSKKAAPKKAKKSAKKAAPKKAKKAAKKSGA
                     KRKPNAAFMKPMTPTASLAAVIGGSPMPRTEVTKKLWAYIKRNGLQDSKNRRNINADD
                     KLQDVFGGKKQVSMFEMTKLVSRHLK"
     gene            4912..5565
                     /locus_tag="VE030_08645"
     CDS             4912..5565
                     /locus_tag="VE030_08645"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HZF23518.1"
                     /translation="MRLTEEEQSLLSGRVAGLEARTGAQTVAAVIGKSDSYPEAPWKA
                     FALGTALAALYCAARSLIAYDWEPGDSALEHAVVILGSGAVLALLAVLLEPVARLFTD
                     RRRRDLEVTQYAKALFFDRGLDRTRGRTGILLLVSLFERKVVILADDGFEARIDRDDW
                     QRLTDRMTLLLSRGNVAGALHAGLEGLEALLLERGYRVDESAADELPNAVLQLKEER"
     gene            5562..6512
                     /locus_tag="VE030_08650"
     CDS             5562..6512
                     /locus_tag="VE030_08650"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HZF23519.1"
                     /translation="MSRIACALIALCWLAAPMAAQDTAIPYLSGRVVDQAGVLSTQAR
                     ERLATMLQAHERATGNQIVVLTVPTLGGESVEDYAVRVFAEWKLGSRDKDNGVLIVVV
                     PQDRRVRIEVGYGLEGTLTDATASRIIRDLMTPRFREGNYDVGIEAGVTAVIQILEGK
                     RSAGPDADWLAPEGQRSGFHVRAPDMPLAERILIGAFVFGIIGLFTFIGLVTPGGMGW
                     FLYFFLIPFWSMFPMFIFGSGAALKILGSYAVLYPLTKVFVSRQPWYRKAREDLKNKG
                     YAEIGGMVIRPGGGSRGFSSGGGFSGGGGRSGGGGASGSW"
     gene            complement(6661..8772)
                     /locus_tag="VE030_08655"
     CDS             complement(6661..8772)
                     /locus_tag="VE030_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HZF23520.1"
                     /translation="MKDVLREALRTVLDPKSVAIIGASENPDKLGGRPLLYLSRFGYK
                     GSVYPINSKRANVQGYKAYPSLTDLPEAPEVAIVVVPGDAAVEAVEQCARLGVRVVIV
                     MTSGFGETDAGAGKAKERHMREVARARGMRVIGPNCQGLANFGSGAILSFSTMYIEAP
                     PQDGPVGIVSQSGAMSVVPYGLLRQRGIGVRHSHATGNDCDVTVAELASVVAEDPDLK
                     LLLLYLEGISDTGHLAETARIARERDLPVIALKSGRTSAGQEAARSHTGALANEDRLV
                     DTFLAKHGIWRARDTAELVGSVEMYLKGWKPKGRRLVAISNSGAVCVMAADAATAVGM
                     PMAKIADQTRAELAKILPGFATTTNPIDITAALLTNSRLFSDILPVLAHDPAADAFLI
                     GIPVAGQGYDVDAFARDSAEFARATGKPLVAAVPQASIAAKFREHGLPVFTTEAEAVA
                     ALNQFLSHRELMQSARERGAALARVPHPLTGGTLLNEADSLARMARAGVPVVGHRLCK
                     SADEAAAALAELGAPVVVKGCSPEVVHKSELGLVRLGLDNEQAVRAAFRDMERTLRGQ
                     GARFDGAIVAKMASGRREMMIGARMDPVFGPVVLVGDGGKYVEAMPDVQVLLSPFNDS
                     EVKAALARLRIAPLLTGVRGEPALDIDAFCAAVLAICRLMMNPESRVSNLDLNPVLVG
                     AKGEGCVALDAVVYATVTSDG"
     gene            complement(8783..>8964)
                     /locus_tag="VE030_08660"
     CDS             complement(8783..>8964)
                     /locus_tag="VE030_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23521.1"
                     /translation="EKQYEERRIVVKAGELFAHLRRQYLLRPIIDPSNVSVRAFSLPL
                     SFLRVLRGSLLFSAY"
BASE COUNT         1610 a         2882 c         2743 g         1729 t
ORIGIN      
        1 acgacatcag aaacaacttg tcagtgccga acgacgtgcc tcgtgtaatt ggaaccttgt
       61 tatcggcggc cacaatggga gacaatgaaa cgtgattccc atccgcgaca ccatccccag
      121 ccgtagtgcg cctgtggtca cctgggcgct catcgcggtc aatgccactg tttttctctg
      181 cgagctccag ttcgccccgg aacagctgga gcagtttttc tacttgttcg gcatggtccc
      241 ggctcgctac agccatcccg aatgggcgcg cgcaatcgga ttcccgatcg acgactactg
      301 gcctttcctg accagcatgt ttctgcacgg aggctggctg cacctcatcg ccaacatgtg
      361 gactctctgg ctcttcggca aagccgtcga aaaccgcatg ggccgaatgc gtttcctcct
      421 gttctacctg ctcaccggcc tcgccgcagg cctcacgcac tggttcacca acgtcgactc
      481 gacgatgcca gcagtcgggg cgtcgggtgc gatagctggc gtgctcggcg catatttcat
      541 tctttatcct cgcgcccgca tcatcgtgat acttccggtt ttcttcctgc cgtttttctt
      601 cgaacttccg gccctcatct atatcggttt ctggtttctg agccaggtgt tcggcggcac
      661 gctgagcggc ctcgggccgg acgatgtcgg aggcgtagcc tggtgggcgc acgtcggcgg
      721 cttcctcgcc ggcgttgtgt tgtatcggct atttctgctc ccgcccggac gtgcaccgcg
      781 caaatacgag ccggatgagt acgacatcga gcagacttgg gctcgatagt gttgacgaag
      841 tgaaggtcat gaaaggatag cgaatggaca ttggtcagct catctggctt ttcttcatgc
      901 tgacggcgct ccaaccggtc atcaagcagc ggttgctcga gagctccagg cagcgcctca
      961 tcgcgaggat cgagaatcgg cgccgatcac gcgtcatcct gctggtgcac cgccaggaga
     1021 caatgagttt cctcggcttt ccgctcatgc gctacatcga catcaacgat tccgaggacg
     1081 tgatgcgcgc gtgcgagatg accgacccgg aagttccgct ggacattgtg ctgcacactc
     1141 caggcgggct cgtgctggcg tcgcttcaaa tcgcgcgcgc catccgcagg cacaagggca
     1201 aggtgacggt attcgttccc cactatgcga tgtcgggagg aacgctcatc gcgcttgccg
     1261 ccgacgagat cgtcatgtcg ccgcacgcgg tgttgggtcc ggtggacccc cagctcggac
     1321 agttccctgc cgcgtcgctg ctcaaggtgg tgtcgaggaa gccgattgcc gaagttgacg
     1381 atcagaccct catcctggcc gacgttgcgg agaaggcaat caaccagctc cgcgtgagcg
     1441 tcaaagagat actgacgagg agccagacac ccgagaaggc agaggagttg gccgatctgc
     1501 tggcgaccgg cgtctggacg cacgactatc caatcacaga cgaggaagcg aggcggttcg
     1561 gactcaaggt cagtacggaa atacccgcgg acgtgttcca gctcatgggc ctgtacccac
     1621 agcctttgcg gcggcaacct tcggtggagt atctgccgat tccccgcagg gcgcagcgga
     1681 ccaagccggg aagtaccact gaatagccgc tggaggactc cagcaggaat acggcagcaa
     1741 ttgccgcaag tccttagcta attgcgtttc gcaattgacg tcgtctctga aaggcagccg
     1801 ttgcacaatc tcatcgcggg gatgagcgca gttgtcgctc tggcggcaac ggggtccaac
     1861 gcggccgagc cactgctgac cgccgggctg taccagatcg aggtccgcat cgccctaccc
     1921 aacgtgcagg atgtggtggc gccgatggtc ttgaaccgat gcctgagcgc tgacgatctg
     1981 aaatcggggc aggcattttt cgtccggagc gacaatccgc tcaggaattg cgaccagctc
     2041 gactaccagg taaccggcgg tacggccagc tatcgtatcg cgtgcccggg accgaacaag
     2101 ggcagcgcag ttgccgtatt cgagaccaca gcaatcacgt atcgcggcgg catcaagatg
     2161 aacatgggcg ggaagaacat gaccatgtcc gagacgcagg tggcagaaaa atctgcgacc
     2221 gccgctagcg accctgcgat accccagggc atcggtccgc acagcaatgt cataatgccc
     2281 attgtccgga tcaatgtcgc cgccatggga ctcgcccgtt tcctgcgccg ctgcgccctg
     2341 cctgcctgct gcctgttggc cgccgccacc tggctggtcg ccgcggaagc tccgcgcctc
     2401 gatccagcgc agttgtccgg cggcattctt accgtcgccg acgaaagcga ggccgcttac
     2461 ctgcaaccgg ctccgtcgct cgacggggag gcgctgaagc ttttcgccga aggccacaag
     2521 tttttcaaca cgcaatgggc gttctactgg ttcgagggcg gcatgtgggg ccgcgggccg
     2581 acttcgaacg ccgattcctg cgttgcctgc catcgcggca acggccgcgg ttctccggac
     2641 gcaccgtcgc gcgatgcgtc ggcgcaacgc gcgcacgagt gcagcgcacc gccgcaaggg
     2701 cctgagcgcg acagttcgcc gcagctcctg tgccgccgca tcgaggcttc cgcgctgtcg
     2761 ctgatcttgc gcctgagcct gcctggcgag aacgcgcatg gcgggcccag gccgcatccg
     2821 cactacggcg accagctgca gaccttcggt gtgccgcggt tgttacccgc cgaaggcacc
     2881 gtacaagtca cctggaacga gcgcagtgtc acgctggcag acggcgaagt cctgcaactg
     2941 cgaacgccgc agatccgcat caccggcctg gcattcggac cgttgggtga ggacatcatg
     3001 atctcgccgc ggatggcgcc gccgctgatc ggcatgggcc tgctggaagc cattccggac
     3061 gcaaccatca tcgccctggc cgagcgcgag ccgaacgacg gcattcgcgg caagaccaac
     3121 gtggcgtggg acatgatcgc aggaaaaact tccatcgggc gcttcgggct gaaggccaat
     3181 cagccgaccc tgcggcagca gattgcggct gcgttcatcg gcgatctggg cctcagcaat
     3241 gacctgtttc cggagcagaa ctgtccgccg atccagaaga cctgcaagga aatgatgttc
     3301 gccggcaatc cggaaatcac cgtccaccgg ctgaccgcgg tggaaacgta tctgcgcacg
     3361 ctggcggtgc cggcgcgccg caacgcggac gacccgcaag tcgtgcgcgg cgcgcaagtc
     3421 ttcgagacgg cgcgctgcgc cgtctgccac gttccgcaac tgcagaccgg ggagttcccc
     3481 gcggcacctc tgctggccca tcagaccatt cgtccctaca ccgacctgtt gctgcacgac
     3541 atgggcgaga acctggcgga ccaccgtccg gactaccggg cgggcggcag cgagtggcgc
     3601 acaccgccac tatggggttt tgggctatcg gagaaagtaa acggccatgg cgcgctgctg
     3661 cacgatggcc gcgcgcgtaa taccgcggag gcgatcctct ggcacggcgg cgaggcggca
     3721 gtctcgcgcg atgccttccg tgccatgccg aaggccgatc gcgatgccct gctcgccttc
     3781 ctcgcttcgc tgtaaattca tttttcaccg caaagacgca aaggacgcga agaaagacaa
     3841 gatgccttta tcaaagggcg agacacggaa atcccactca ctcaccctct aacatttatc
     3901 gtggttcttc ttccgttcag tttgtttctg tttcctttgc gctctttgcg tctttgcggt
     3961 gagaatggtt ttcgttctag ctctggcccg acgctagtcg tagacagcga gatgagcgcg
     4021 tagatccacg cgaacatggc gcctcaggtc acattgcgct gtacgccagc tcggtgcggc
     4081 agcacgtgcc cttgcagctc atgaaatcca gtgtatcagg tgaggaagcg cggcaaaccc
     4141 ctcacctcgt gggaacgagg caaggcaaaa aaaactgtcg cggcagcaaa tgccgactgc
     4201 ccggaccggc atccgccggc ccgggtagcc gggttcagca ggagaagctt atttgaggtg
     4261 ccgggagacc agtttggtca tctcgaacat cgatacctgc ttcttgccgc cgaagacatc
     4321 ctgcagcttg tcgtctgcgt tgatgttgcg ccggttcttg ctgtcctgca ggccgttcct
     4381 cttgatgtag gcccacagtt tcttggtaac ctcggtgcgc ggcatggggg aaccgccgat
     4441 cactgcagcg agcgacgcgg tgggcgtcat cggcttcatg aaagccgcgt tgggcttgcg
     4501 cttggcgccg ctcttcttcg cagccttctt cgcctttttc ggggctgctt tcttcgcgga
     4561 ctttttggct ttcttcggtg ccgccttctt ggatttcttg gcggacttcc tcgcggactt
     4621 cttctttgtg gccattgtga tgcctccagt ctagggtttg gtcatgtctt aatctcaatt
     4681 gccttgccag aagcaaaggc acggcgagca gagaatgccg agtggttgca gcgttgtcaa
     4741 tcagtgaatg cacgtttttc ataggggaag caccgacgaa tcgcatcttt ttgaggccct
     4801 tctcctcctg aatacggtct ttcgccagtt tgcatcggcc gcaaaccgct tccagaaatg
     4861 cctcccgggc gttcacaata cgtgtcagca cccattccgc agacacagcc gatgcgcctt
     4921 accgaagagg aacaaagcct gctttccggc agagttgccg gactcgaagc ccggaccggc
     4981 gcccagacgg tggccgcagt aatcggtaaa tccgactcct acccggaggc gccgtggaag
     5041 gcgtttgcgc tcggcaccgc gctggcggcg ctctattgcg cagcccggtc gcttatcgcc
     5101 tacgactggg agccagggga ttccgcgctc gagcacgccg ttgtcatcct cggcagcggg
     5161 gcggtgctgg cgcttctcgc cgtgctgctc gaaccggttg cgcgactgtt cacggacaga
     5221 cggcgccgag acctcgaagt cacccagtac gccaaggcat tgttcttcga ccgcggactt
     5281 gaccgcacac ggggccgcac cggcattctg ctgttggtga gcctgttcga gcgcaaggtc
     5341 gtcattcttg ccgacgatgg gttcgaggcg cggatcgacc gcgacgactg gcagcgactg
     5401 accgaccgga tgacgctgct gctcagtcgc ggcaatgtcg ccggagcgtt gcacgccggg
     5461 ttggaagggc tggaggcatt gctcctggaa cgcggctacc gcgtcgacga aagcgccgcg
     5521 gacgagcttc cgaacgcggt gctgcagttg aaggaggaga gatgagccgg atcgcttgcg
     5581 cgctcatcgc cctgtgttgg ctcgccgctc cgatggctgc gcaggacacg gctattccct
     5641 atctgtccgg ccgcgtcgtg gaccaggccg gggttctttc aacccaggcg cgcgaacgct
     5701 tggccaccat gctgcaggcc cacgagcgag ccaccggaaa ccagatcgtc gtgctgaccg
     5761 tcccgactct gggcggggaa agcgtcgagg actatgcggt gcgggtgttc gcggaatgga
     5821 agctcggcag ccgcgacaag gacaacggtg tgttgatcgt ggtggtgccg caggatcggc
     5881 gcgtccgcat agaggtcggc tacggactcg agggcacgct caccgacgct accgccagcc
     5941 gcatcatccg cgacctgatg accccgcgct tccgcgaggg caactacgac gtcggcatcg
     6001 aggccggggt caccgccgtt atccagatcc tcgagggaaa gcggtccgcc ggtcccgacg
     6061 ccgactggtt ggcgcccgag gggcaaaggt ccggtttcca cgtgcgcgct ccggatatgc
     6121 cgctggccga gcgcatcctg atcggcgcct tcgtgttcgg cataatcggc ctgtttacct
     6181 tcatcggcct ggtcacgccg ggcggaatgg gctggttcct ctacttcttc ctgatcccgt
     6241 tctggtccat gttcccgatg ttcattttcg gcagtggcgc cgcgctcaag atactcggca
     6301 gctacgccgt gctctatccg ctcaccaagg tcttcgtttc ccgccagccc tggtatcgca
     6361 aggcacgcga agacctcaag aacaagggct atgccgaaat cggtggcatg gtcatccgcc
     6421 ccggcggcgg ctcgcggggt ttctccagcg gcggcggttt ctcgggggga ggcggacgtt
     6481 cgggcggtgg cggcgcgagc ggaagctggt aaatgcggtg atgggttatg ggtgatgggg
     6541 taacgaatga caacaggcag gtgccgagtt acagcggccg agtaatgacg taaaggcggt
     6601 ccaaaacgcg gtttccttgc cctttaaatc acggatcttc ggagagtctt tttttgccca
     6661 tcacccatca ctcgtcaccg tcgcgtatac gaccgcatcc agcgcgacac atccctcccc
     6721 cttcgcgccg accagcaccg gattgagatc gaggttcgac accctcgatt cgggattcat
     6781 catcagccgg catatcgcca gcacggccgc gcagaacgcg tctatatcca acgcgggctc
     6841 gccgcgcacc cccgtcagca gcggcgcgat gcggagcctg gcgagtgccg ctttcacttc
     6901 actgtcgtta aatggcgaca gcaacacctg cacatcgggc atcgcttcga cgtactttcc
     6961 gccgtcgccg accagcacca cgggaccgaa caccggatcc atgcgcgcac cgatcatcat
     7021 ctcccgtcgg ccgctggcca tcttcgccac gattgcacca tcgaaacgcg cgccctgccc
     7081 gcgcaaggtg cgttccatgt cccggaaggc cgcgcgcacc gcttgttcat tatcgagacc
     7141 gagccgcacc agtcccagct cggacttgtg caccacttcg ggtgaacagc ctttcaccac
     7201 tactggagcg cccagctcgg ccaaagccgc cgccgcttcg tccgccgatt tgcacagacg
     7261 gtgcccgacc accggtacgc cggcacgtgc catccgcgcc aggctgtctg cttcgttgag
     7321 cagggttcct cccgtaagcg ggtgaggcac gcgtgcgagc gcggcaccgc gctcgcgagc
     7381 gctctgcatc agttcgcgat gcgacaggaa ctggttgagc gcggcgaccg cctcggcttc
     7441 ggtggtgaat accggcaagc catgttccct gaatttcgcc gcgatgctcg cctgcggcac
     7501 cgccgccacc agcggcttgc cggtggcacg ggcaaattct gccgaatcgc gcgcgaaggc
     7561 atccacgtcg tagccctgcc ccgccacggg aatgccgatc aggaaggcgt cggccgcggg
     7621 gtcatgcgcc agcaccggca ggatgtccga gaacagcctg ctgttggtca gcagcgccgc
     7681 agtgatgtcg atcggattgg tggtggtggc aaagcccggc aggatctttg ccagttccgc
     7741 gcgggtctgg tcggcgatct tcgccatcgg catgccgacc gcggtggcgg cgtctgccgc
     7801 catcacgcac accgcacctg agttgctgat tgccaccagc ctgcgtccct tcggcttcca
     7861 gcctttcaga tacatctcga cgctgccgac cagttccgcg gtatcgcggg cacgccagat
     7921 gccgtgcttc gccaggaagg tgtccaccag gcgatcctcg ttggcgagcg cgccggtgtg
     7981 cgagcgcgcc gcttcctgtc ctgccgacgt gcgcccgctc ttcagcgcga taaccggcag
     8041 gtcgcgctcg cgggcaatgc gcgcggtctc ggcgaggtgc ccggtgtcgc tgatgccctc
     8101 gagatagagc aacagcaact tcaggtccgg atcttccgcc accaccgacg ccagttcggc
     8161 gacggtgacg tcgcagtcgt tgccggtggc gtgcgaatgg cgcacgccga tgccgcgttg
     8221 gcgcagcagg ccgtaaggca ctacgctcat cgcgcccgac tggctgacga tgcccaccgg
     8281 gccgtcctgg ggcggcgcct cgatgtacat ggtggaaaac gacagaatcg cgccgctgcc
     8341 gaagttggcc agcccctggc aattcggccc gatcacgcgc atgccgcgag cgcgcgcgac
     8401 ctcgcgcatg tgacgttcct tggccttgcc ggccccggca tcggtctcgc cgaaaccgga
     8461 agtcatgacg atcaccactc tcacacccag tcgcgcgcac tgctcgaccg cctccaccgc
     8521 ggcatcgccc ggcaccacga cgatcgcgac ttccggcgcc tcgggcaaat cggtcagtga
     8581 cggataggcc ttgtaaccct gtacgttggc ccgcttcgaa ttgatcggat acacgctgcc
     8641 cttgtaaccg aaacgggaca gataaagcag cgggcgtcca cccagcttat cgggattttc
     8701 tgaagccccg atgattgcga cggacttcgg atccagcacc gtgcgaagcg cctcgcgcaa
     8761 cacgtctttc attctttatc cttcaatacg cagaaaaaag cagtgaacca cggaggacac
     8821 ggaggaaaga caacggcaag gaaaatgctc ggacggaaac gtttgagggg tcgatgatcg
     8881 gtcgcagtaa atattggcgg cgcaaatgtg cgaacagttc tcctgccttg accacgattc
     8941 tcctttcctc atattgtttt tctc
//
LOCUS       DASQOP010000213        11734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1508289, whole genome shotgun sequence.
ACCESSION   DASQOP010000213 DASQOP010000000
VERSION     DASQOP010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11734
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1508289"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VE030_08665"
     CDS             <1..265
                     /locus_tag="VE030_08665"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HZF23522.1"
                     /translation="SGMVFSYLEAGVNAMWMKNTRIPLSVAFIDRDGRILNIADMEPF
                     SEQAHASAGEAAYALEMNRGWFRKRGVKAGDRVEGLRRLPPPR"
     gene            complement(316..795)
                     /locus_tag="VE030_08670"
     CDS             complement(316..795)
                     /locus_tag="VE030_08670"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HZF23523.1"
                     /translation="MEMLPGIYRWIHILAGVMWIGLLYYFNFVQVAALKAAQADGTAA
                     GITKHVAPRALLFFRWAAVVTWLAGAALLGAHFVPAFTFQAGWEKIGMGAWLGTIMLI
                     NVWALIWPNQKKILGLVQASDAEKNKARRVAFLTSRVNTMLSIPMLFFMVAQSHIGM"
     gene            complement(1176..1970)
                     /locus_tag="VE030_08675"
     CDS             complement(1176..1970)
                     /locus_tag="VE030_08675"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HZF23524.1"
                     /translation="MISEDPAATSPIRLGIMPGKPLLQLRFGHASITVPGKSNEDFYG
                     IVTVREEPEAQSRGIAVAIADGVSGSGGGRLASETTVRSLLRDFYGAPVSWNTAPTID
                     KVLRSVNDWLAAENARNPELEGVVSTLTMMLFKGNYYHLAHVGDTRAYRKRGRVLKQL
                     TIDHTWQRGDMRHVLKRAVGLDTHLVVDYSDGELVPGDVFVMVTDGVWEVLGERVVRE
                     VLDAGGDVQAIADELVQRSVRNQVQYMGRNDATAVVAAVDNSERQS"
     gene            complement(2038..3066)
                     /gene="ybiB"
                     /locus_tag="VE030_08680"
     CDS             complement(2038..3066)
                     /gene="ybiB"
                     /locus_tag="VE030_08680"
                     /inference="COORDINATES: protein motif:HMM:NF006005.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HZF23525.1"
                     /translation="MSVARYIRKLTDGSDGERDLTEEDAHDLFAAMLDGGVPDLELGA
                     CLVALRIKTGSAAELLGFYRAASERLYTLRPPATSLRPLIFATYNGARHEPNLLPLLV
                     LLLQRIGIPVLLHGTLEGSGRVASIYILRELGVLPSGSLAQAQRSLDDELLAFVPTAV
                     LCPGLANLLSLRSRLGIRNPAHDLVKLLDPFAGAGLRVVSASQAPHLVAAEAFLAATG
                     FDALLLRSTEGESFANPRQRPKIQLFRHGDTSVLFEEEWAPAKHIPSQPASIEAAVTA
                     QWIRQALAGQVPVPHPLVNQLACCLYAAGYTEDMNQAKAIAAVEAGGLTPSTPGRSAE
                     GMRPRTNT"
     gene            3718..4773
                     /locus_tag="VE030_08685"
     CDS             3718..4773
                     /locus_tag="VE030_08685"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZF23526.1"
                     /translation="MNFETVSDPLLRFALAAGLAVLVLSALMTLHILLLRLQLTLRQR
                     RENRFTARWRPRFLESMQAIPLRLPRIAHANWFTFLSLWNQYQESGSATARQRLKTLI
                     LQLRMDVAARKLLARGSIRVRLMAVLTLGHLGDRESWDTLHELSQSPHPLLSLAAARA
                     LLLIDASAALPVLLPAFTVRGDWPLARVETMLADAGAHKVSAQLAAAVDLTPPERLPR
                     LVALMAVARSDDAMPRIRELLEMSENEEVLTACLKSVHVPPDRELIARFARHASWQVR
                     TQAARALLRAARAGDEKLLVQMLADPVWWVRYRAAQSLVALPFLSNDDLWRLRWTLED
                     KFGVNMLDQVMAERKSP"
     gene            4770..6230
                     /locus_tag="VE030_08690"
     CDS             4770..6230
                     /locus_tag="VE030_08690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZF23527.1"
                     /translation="MNWQMSDLATWVGAMQWMFLLYFIGANGGYSLLNLLSLAGIRRH
                     LEERALEALPHIYSGFEPPVSILVPARNEEATIAASVRSLLQFEYPEFEVVVVNDGST
                     DGTLAALTREFALAPFPEAYWKRLPIQPLRGIYRSALHPNLRVLDKVNGGKADALNAG
                     INAARYPLYCALDANSILQPDSLKRVVQPLVQDPATVASGAAARIANGCTVNDGFIAE
                     IDLPGHPLALIQLVEYLRAFLFGRLGWSPMNAVLILSGALAVFRKEAVVGAGGYRCDV
                     VDEDMELVVRLHRLNRLAGLPYRIALVPDPILWTEVPESLVVLAKQRVRWQRGLSEAL
                     FLNIRLLLHPRGGAAGWLAFPFTALFEWLGPLLEVAAYVLMTAAWFFGLVSAQAFVAF
                     MLVAIGMGIMVSVGALLLEELSFHTYRKPGQLAWLLGAVIMENCGYRQLVAVWRLAGL
                     LRWMGGSATHRGEITGDSGWSKRRRPTAESTGRRRP"
     gene            6227..6361
                     /locus_tag="VE030_08695"
     CDS             6227..6361
                     /locus_tag="VE030_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23528.1"
                     /translation="MIYDLPFYRANFKRGPVAGLGVELPTYPEAADLAATIGSIVLSR
                     "
     gene            6361..7797
                     /locus_tag="VE030_08700"
     CDS             6361..7797
                     /locus_tag="VE030_08700"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HZF23529.1"
                     /translation="MNFKLYIPRPILAVLAVLGVLFAIDLYALAAYRQLEQAADELRT
                     RSEMLEALKPLPEIVLQMQSGMRGYLLTGDEAFLARYRAASESFPAAVAHALALVGDD
                     RAQRARLEEAARLTQEWLATYLSPLVVKRSASEGAHSTMAAVLNTVREGKGEALADRI
                     TGQLEEIERSEAARVAGAEGRIREELGSVAQWVLARGAALLLALAALALLLGRTIERL
                     GGQIRGREVAERAARQSEAARRAMDDASPLGMFVADVSGSCTHANAAFARISGLAQSA
                     LDGHGWQTVLHPDDRDRVLPAWNQAIAQHAGFASEHRFLHRSGRVVWVTMKAARISDG
                     DQLIGFVCTVEDVTERREAEEALRKSEERLHLALESSGLALFDWHLPSGEIFLSRQWG
                     VIAGDRQEPVTTTARRLAELVHPEDRERLREASIGAFKGSTPSFHAEFRIQVHGGPWK
                     WVYSQGQVTERDSIGRAVRLTGTIGADE"
     gene            8087..9295
                     /locus_tag="VE030_08705"
     CDS             8087..9295
                     /locus_tag="VE030_08705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZF23530.1"
                     /translation="MRPELSLLFRAAAAAVTLAALPACQPSQSNPANAEMPPEVSAVV
                     VQPRDLPATFEYVGQIAGVREVEVRPRVSGILERWNYQEGAKIAAGQSLFTIDSAPYR
                     AALARAEAQLASAEASLAQSSRDAARLKPLFEAKAVSQKDLDDAVSSAQVASANVKAA
                     QAAVTEARLNLSYTRVEAPISGITSRALQSEGSLVQAQQSLLTTISQIDPIHVIFSFT
                     ETEHLKFTRAVEEGRLVLPKGGKFDVKLKLADGSEYARAGKVDFTDIRVDPNTGTIEA
                     RAVIANPQGLLRPGQFARVALTGGVRPAAIAIPQRAVLEGPGTKIVLTVNAQGIVEPR
                     PVQVGDWSGEDWIVLGGLKAGDQVIVDGVVKARPGSPVKIAQAPPANPAQPGQVPAAK
                     PDAAQQPAKQ"
     gene            9314..>11734
                     /locus_tag="VE030_08710"
     CDS             9314..>11734
                     /locus_tag="VE030_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZF23531.1"
                     /translation="MFSRFFIDRPIFAAVLSIFLVLAGLASMRTLPIAQYPEIAPPVV
                     TVRAVYPGASAEVLEQTVAAPLENQINGVENMLYMASNSSSNGVVEINVTFEIGTNID
                     QAALNVNNRVKQAEARLPLEVRRQGVTVEKNSSSFLQVLAFNSPDGTFDDLYVSNFVT
                     LNVLDTIKRIPGTTNVQIFGAKDYAMRIWLRPDRMTQLKLTTGDLLRVLNEQNAQFAA
                     GKIGQAPTGGTQEMVYTITTKGRLADAREFENIIVRSNPDGSALRLKDVARVELASKD
                     YDFIGRVNGKESALLGIFLQPGANALDVAKDVGRTMQALSKRFPAGMEYQIVYDTTRF
                     VEVSIREVVKTLAEAMALVFLVVYLFLQNWRATLIPFAAVPVSLIGTFAGLFLLGYSI
                     NTLTLFGMVLAIGIVVDDAIVVLENVERIMHEEHKNAREAAIKAMSEVTGPVIAIVLT
                     LCAVFVPIAFLGGLTGELYRQFAVTIAIAVVLSGIVALTLTPSLCVLILKREHKAPGR
                     FFSWFNDWFRRVTGRYTGGAGWIIRRGAIGMVLFAVMVVVAAGLWKITPGSLVPDEDQ
                     GYYIGAVFLPDGATLQRTDRVVSEVLKAVQSNPANEYAVAFTGLDFIGGGFRNSAATI
                     FVTQKHWDERPGVGTQQLIGDYFMKTAHIKEGLVLAFAPPPIFGLGNAGGFEFYIQNR
                     GEGGPQRLAQEMGKFLAAANKDPQLAGVQTLWRAGVPQLHVDVDREKAKALGVPLNDL
                     FDTLAATLGSYYVNDFNKFGRTWQVLMSAEPSFRMRPDSIGELYVRSEKKGMVPIRAL
                     ANVSYTSGP"
BASE COUNT         2042 a         3780 c         3766 g         2146 t
ORIGIN      
        1 cagcgggatg gtgttcagtt accttgaagc cggcgtcaac gcgatgtgga tgaagaacac
       61 ccggattccc ctgtcggtcg cgttcatcga tcgcgacggc cgcattctca acatcgcgga
      121 catggaaccg ttctcagagc aagcgcacgc ctccgccggc gaggcggcct acgcactgga
      181 gatgaaccgc ggctggttca ggaaacgcgg cgtcaaagcc ggcgaccgtg tcgaaggctt
      241 gcgacgcctg ccgccgccaa gatgaaaaac gcccgggcac gggcccgggc gttgccatgg
      301 catccaccgc ggtttctaca tgccgatgtg cgactgcgcc accatgaaga acagcatcgg
      361 gatcgacagc atggtattga cccgcgaggt gaggaaggcc acacggcgcg ccttgttctt
      421 ttccgcgtcg ctggcctgaa ccaggccgag aatcttcttc tggttcggcc agataagcgc
      481 ccacacgttg atgagcatga tggtgccgag ccacgcaccc atgccgattt tttcccaacc
      541 ggcctggaag gtgaacgcgg gaacgaagtg tgcgccgagc agtgccgcgc cggccagcca
      601 ggtcaccacc gccgcccagc gaaagaacag caatgcgcgc ggggctacgt gcttggtaat
      661 tccggccgcg gtaccgtcgg cttgcgcggc cttcaatgct gctacttgaa cgaagttgaa
      721 gtagtaaagc aggccgatcc acatgacacc ggccaggatg tggatccagc gataaattcc
      781 cggaagcatt tccatggctc aacccccgat cagagacttg acgacgtaat acaggataac
      841 ggtgaggatc agtccggcga tcaccgtccc ccaaagcgaa tccagcgggt tcttcattgc
      901 ttgctccccc tttcgatgta agcgacaccg gcagtgtagg cgacggccca aatgggagca
      961 acagtcgaac gtcatgggcg gctaatccct ttccaagcaa ggggcaccgg gatgcaaagg
     1021 gaagcggctc gcgcggcatc cccgcgtctt taggggcccg cttcagccgc gcagatcagg
     1081 taaaaccctg tcgatgatat tccggtgcgg tcctaagccg gtccgggcaa ccccaatcgc
     1141 ctcggccgcg cacgtggaac ttgctcagag cttccctagc tttggcgttc agaattgtcc
     1201 accgccgcca cgaccgccgt ggcgtcgttg cgtcccatgt actggacctg gttcctgacc
     1261 gatcgctgca ccagctcgtc ggcgatggcc tgcacgtcgc cgcccgcatc gagcacttcg
     1321 cgcacgaccc gttcgcccag cacctcccag acgccgtcag ttaccatgac gaagacatcg
     1381 cctggcacca gttcgccatc gctgtaatcg accaccagat gcgtgtcgag tccgactgca
     1441 cgcttcagca catggcgcat gtcgccgcgc tgccaggtat ggtcgatggt cagttgcttt
     1501 aatacccgcc cacgcttgcg gtaggcgcgg gtatcgccga cgtgggcgag atggtagtag
     1561 ttgcctttga acagcatcat cgtaagcgtg ctgaccacgc cttccagctc gggattgcgc
     1621 gcattctccg ccgccagcca gtcgttgacc gatcgcaaga ccttgtcgat cgtcggcgcg
     1681 gtgttccagg ataccggcgc gccatagaag tcgcgcagca ggcttctcac ggtggtttcc
     1741 gaagcgagcc gtccgccgcc gcttccgctt acgccgtcgg cgatcgccac ggcaatgccc
     1801 ctgctttgcg cctccggctc ttcccgcacg gtaacgatgc cgtagaaatc ctcgttgctc
     1861 ttgcccggaa cggtgatgct ggcgtgaccg aatcgcagtt gcagcaaggg cttgcccggc
     1921 atgattccga ggcggatcgg actcgtcgcg gcaggatctt ctgaaatcat ttgtccctcg
     1981 gtgccagatg tgtgactcgg tgcgaatggg cgtgcggctt ggcgtcgcat cgattcttca
     2041 ggtattggta cgcgggcgca tgccctcggc ggaacggccg ggtgtagacg gcgtcagccc
     2101 gcccgcctcg accgcggcga tggccttcgc ctggttcatg tcctcagtgt atccggccgc
     2161 gtacaggcaa cacgccagtt gattaactag cggatgcggc accggtacct gcccggcgag
     2221 cgcctgacgg atccactgcg ccgtgaccgc ggcttcgatg ctcgccggct ggctcggtat
     2281 gtgtttggca ggagcccatt cctcctcgaa aagcacgctg gtgtcgccat ggcggaacaa
     2341 ttggattttc ggccgctgcc gggggttggc aaaggactcg ccctcggtgc tgcgcaacaa
     2401 cagcgcgtcg aagccggttg cggcaagaaa cgcctctgcc gcgacgagat gcggcgcctg
     2461 gctggcgctt accacgcgca agccggcgcc cgcgaacgga tcgagcagct tgaccaggtc
     2521 gtgcgcggga ttgcgaatac cgagccggct tctgagcgac agcagatttg caagtccggg
     2581 gcataagacc gctgtcggta caaatgccag caactcatcg tcgagggacc tctgcgcttg
     2641 cgcgagcgac ccactcggca acacgccaag ctcgcgcaat atgtatatgc tcgccacccg
     2701 tccgctacct tccagcgtgc cgtgcaatag cacagggatg ccgatccgct gcagcaacaa
     2761 caccagcagc ggcaacagat tcggctcatg ccgggcgccg ttgtaagtcg caaagatcag
     2821 cggcctgagg ctggtggcgg gtggccgcag cgtgtacagg cgttccgacg cggcccggta
     2881 aaagccgagt agctccgcag cggagccggt tttgatccgc agcgccacca ggcacgcacc
     2941 gagttcgaga tcgggcacgc caccatcgag catggcagcg aaaaggtcgt gagcgtcctc
     3001 ctcggtcaag tcccgctcgc catcactccc gtcggtgagt ttcctgatgt aacgcgcaac
     3061 gctcatggct tcccgtcaaa accgcagtga ttgtcgtcgg gtgcgcgata ctttgggcca
     3121 cgaacccgtc ctacctttgc tgcccacaac aaacgcaacc cccgtgccac cgctcctttc
     3181 cgtgatgaaa caatgcgttg gtgcagaggg catacggaac gtcggaaatg ctggtgcgaa
     3241 acccgatgct ggtgcatcaa cttggtgcgg cgagccgggc ctcctgtcgc ggtagcgcca
     3301 gaaacctggg tcgcctgcgt gcgccagcgg cagactggtc gtgcttacgc cgaagccgga
     3361 ccggtaatcg cgcaggccgc gcgccggggc ccggacgcgg ggtcgcggga cacgcgaggc
     3421 tataatgcaa gccgatatcg cgtcggtaac ccgcgtgcat atatcccgcc agcctttccg
     3481 gtctgctgca ctccatgctc gtcaccgaca acgacgcctc gccgtcgagc tgcggacacg
     3541 aagcttcgtg gatgcctgtc tcggccagct tgcgcgaaat tgcagccgat ggcccgtcga
     3601 ccgcgatgac cgccagcggc tcgacagaga tcccggtccg gcagcaccgg gtgcgcggtt
     3661 ctgcggcgga ccgctcccgg ccgcgcgatc aatgcaaagg gtgcgagggt attcgccttg
     3721 aatttcgaaa ccgtatccga tccccttctg cgcttcgcgt tggccgcagg cctggcagtg
     3781 ctggtgctgt ccgcgctcat gacgttgcac atattgctgc tgcgcctgca gctcacgctg
     3841 cgccagcgcc gtgaaaaccg cttcaccgcg cgctggcggc cgcggtttct ggagagcatg
     3901 caggccattc cgctgcgctt gccgcgaatt gcgcacgcca actggttcac tttcctttcg
     3961 ctttggaacc agtaccagga atcgggatcc gcaaccgcgc gccagcggct gaagacgctt
     4021 attctgcagc tgcgcatgga tgtcgcggcg cgcaagctcc tggcgcgcgg cagcatccgc
     4081 gtacgtctca tggcggtgct cacgctcggt catctgggcg accgcgaatc ctgggacacg
     4141 cttcatgagc tgtcgcagtc gcctcaccct ttgctgtcgc tggcggcggc acgcgccctg
     4201 ttgctgatcg acgcttccgc ggcgctgcca gtgctgttgc cggcgttcac cgtacgcggc
     4261 gactggcccc tggcgcgcgt cgagacgatg ctggcagacg caggcgcgca caaagtctca
     4321 gcccagcttg cggccgctgt ggatctcact ccgcccgagc gactgccgcg cctggtcgcg
     4381 ttgatggccg tggcgcgatc cgatgacgca atgcccagga tccgcgaact gctggaaatg
     4441 agcgagaacg aggaagtcct tacggcgtgc ctgaaatccg tgcatgtgcc gcccgaccgc
     4501 gagctgatcg cgcgttttgc gcgccacgcc agctggcagg tgcgcaccca ggcggcgcgc
     4561 gcgctattgc gtgccgcacg ggcgggcgat gaaaaactgc tggtgcagat gctggcggat
     4621 ccggtgtggt gggtgcgcta tcgcgcggcg cagtcgctgg tggcgctgcc gtttctgtcc
     4681 aacgacgact tatggcgact gcgctggacg ctggaggaca aattcggcgt caacatgctc
     4741 gaccaggtca tggccgaaag gaagtcgcca tgaactggca gatgagcgac ctggcgacct
     4801 gggtgggcgc gatgcagtgg atgttcctgc tctacttcat cggcgccaac ggcggctaca
     4861 gcctgctcaa cctgctgtcg ctcgccggca tcaggcgcca cctggaagag cgtgcactgg
     4921 aggcgctgcc acacatttac tcgggatttg agccgccggt ttctatcctg gtgccggcac
     4981 gcaacgagga ggctaccatc gccgcctcgg tgcgctcgct gttgcagttc gagtacccgg
     5041 agttcgaggt ggtggtggtc aacgacggct ccaccgatgg cacgctggcc gcgcttaccc
     5101 gcgaattcgc gctggcgccg ttccccgagg cgtactggaa gcggctgcca attcaacccc
     5161 tgcgcggcat ctaccgttcc gccctgcatc ccaatctgcg cgtgctggac aaggtcaacg
     5221 gcggcaaggc cgacgccctg aacgccggca tcaatgccgc gcgctatccg ctgtattgcg
     5281 cacttgacgc caactcgatc ctgcaacccg attcgctcaa gcgcgttgtg caaccgcttg
     5341 tgcaagaccc ggccacggtg gcttcgggcg cggctgcgcg cattgccaac ggctgcacgg
     5401 tgaatgacgg ctttatcgcc gagatcgatt tgcccggcca tccgctggcg ctgatccagc
     5461 tcgtcgagta tctgcgcgcc tttctgttcg gccggctggg gtggtcgccg atgaacgcgg
     5521 tgctgattct ctccggcgcg ttggcagtct tcagaaagga agcggtggtg ggggccggcg
     5581 gctatcgctg cgatgtcgtg gacgaggaca tggaactggt ggtgcgcttg catcggctca
     5641 atcgtctggc cggactgccc taccggattg cacttgttcc agatccgatt ctctggaccg
     5701 aagttccgga atcgctggtg gtgctcgcga aacagcgcgt gcgctggcag cgcggcctgt
     5761 cggaggcgct tttcctcaac atcaggctgc tgttgcatcc gcgcggcggc gcggccggct
     5821 ggctggcttt cccgttcacg gccctgttcg aatggctggg gccgctgctg gaagtggctg
     5881 cctatgtcct gatgacggcc gcatggttct tcggactggt gtccgcgcag gccttcgtcg
     5941 ccttcatgct ggtggcaatc ggcatgggta tcatggtgtc agtgggcgca ctgttgctcg
     6001 aagagctctc cttccacacc taccgcaagc cgggccagct agcttggctg ctcggcgcag
     6061 taatcatgga gaactgcggc taccggcaac tggtcgcggt gtggcggctg gccggtctgt
     6121 tgcgctggat gggtgggagc gcaacccatc gaggcgagat caccggggac agcggctggt
     6181 cgaagcgccg gcggccgacg gccgagtcga cgggtcgacg ccggccatga tttacgacct
     6241 gccattttac cgagcgaatt tcaagcgcgg gccggtggcc gggctcggcg tggagttgcc
     6301 cacttatccc gaagcggccg atctcgccgc aacgatcggt tcgatagtct tgagcagatg
     6361 atgaacttca aactttatat cccgcgtccg attcttgccg tgcttgcggt gctcggggtg
     6421 ttgttcgcaa tcgacctgta tgcgctggct gcctatcgcc aactcgagca ggctgccgac
     6481 gagttgcgca cccgcagcga gatgctcgag gctctgaaac cgctgccgga aatcgtactg
     6541 cagatgcaat ccggcatgcg cggttacctg ctcaccggcg acgaggcctt cctcgcacgc
     6601 taccgcgccg cttcggaaag ttttccggcc gcggtcgcgc atgcgctggc gttggtcggg
     6661 gacgaccggg cgcagcgcgc ccgcctggag gaagcagcga ggctgaccca ggagtggctg
     6721 gcgacgtatc tttcgccgct ggtggtcaag cgcagcgcca gcgaaggcgc ccacagcacc
     6781 atggcggcgg ttctgaacac cgtcagggaa ggcaaaggtg aggcgctcgc cgaccgcatt
     6841 accgggcaac tcgaagagat tgagcgttcc gaggcggcgc gcgtggcggg cgcggagggc
     6901 cggatacggg aggagctggg gagcgttgca cagtgggtgc tggcacgcgg agcggcgctc
     6961 ctgctggcgc tggcggcgct cgcgctgctg ctcgggcgca cgatcgagcg tctcggaggc
     7021 cagatccgcg gccgcgaagt cgccgagcgc gcggcgcggc aaagcgaggc ggcgcggcgt
     7081 gcgatggacg acgcgtcccc gctgggaatg ttcgtcgccg atgtctccgg ttcctgcacg
     7141 cacgccaatg ctgcgtttgc gcgcatttcc ggcctggcgc aatcggcgct ggacggccac
     7201 gggtggcaga ccgtgctgca tccggatgac cgcgaccgtg tactgcccgc ctggaaccag
     7261 gcgatcgcgc agcacgcggg attcgcctcc gaacaccgct tcctgcatcg cagcggccgc
     7321 gtggtctggg tgacgatgaa ggccgccagg attagcgacg gcgaccagtt gatcgggttc
     7381 gtctgcacgg tggaggatgt gaccgagcgg cgcgaggcgg aggaggcgct gcgcaagagc
     7441 gaagagcgcc tgcatctcgc cctggaaagc tccgggctgg ccctgttcga ctggcacctg
     7501 ccctcggggg aaattttttt gagccggcaa tggggagtga tcgcgggcga ccggcaggaa
     7561 ccggtcacga ccacggcgcg gcgactggcg gaactggtgc acccggaaga ccgggagcgg
     7621 ttgcgtgaag ccagcatcgg cgctttcaag ggcagtacgc cctctttcca tgccgaattc
     7681 cgcatccagg tccacggtgg gccgtggaag tgggtttaca gccagggcca ggtcaccgag
     7741 cgcgacagca tcggccgcgc ggtgcggctc accggcacta tcggcgcgga cgaataattc
     7801 ggttcagtcc aagggtgcag cgcgcgccgg tagcttgacc ggagcccgtt tcgccgctgt
     7861 ctgggcagca atcccggcgg ttcgttttca gcatttcagc aagaatgtac ttttcatgca
     7921 gtcgcacatg taagtaaact ccgcctgctc tgtggcctgg cgcacagtcg gcgcagcgcc
     7981 ctccggtgtc ccgtcccagt atccagccgc gtccgtccgc ggcgtaaccc ggcgcggatg
     8041 gcaatcatgg gatccctttt gccggaaata ccaatgggag tgtcaagtga gacctgaact
     8101 ttcgctgctc tttcgcgccg ccgcggcagc agttacgctg gcggccctac cggcctgcca
     8161 gccgtcgcaa tcgaacccgg ccaacgccga gatgccaccc gaggtttccg cggtcgtggt
     8221 gcaaccgcgc gatctgccgg caactttcga gtatgtcggg cagatcgccg gcgttcgcga
     8281 agtcgaggtt cgcccgcgcg tgtccggcat cctggagcgc tggaattacc aggaaggcgc
     8341 gaagatcgcc gccggacaga gcctgttcac catcgactca gcgccgtatc gtgccgcgct
     8401 ggcgcgggcg gaggcccagc tcgccagcgc agaggcgagc ctggcccagt cgagccgcga
     8461 cgccgcgcgc ctgaagccgc ttttcgaggc taaggcggtc agccagaagg atctcgacga
     8521 cgccgtctcc tcagcacagg tggcatcggc caacgtcaag gccgcccaag ccgcggtgac
     8581 cgaagcgagg ctgaatttgt cctacacccg cgtcgaagcg ccgatctccg gaattaccag
     8641 ccgcgccctt caatccgagg gcagcctggt gcaggcccag cagtcgctgc tcaccacgat
     8701 ctcgcagatc gacccgattc acgtgatctt cagtttcacc gaaaccgaac acctgaagtt
     8761 tacccgcgcc gtggaagaag gccgcctggt cctgcccaag gggggcaagt tcgacgtcaa
     8821 gctcaaactc gccgacggat ccgaatacgc ccgcgccggt aaggtggact tcaccgacat
     8881 ccgtgtcgat ccgaacaccg gcaccatcga agcccgtgcc gtcatcgcca atccgcaagg
     8941 gctgttacgt cccgggcagt tcgcccgcgt ggcacttacc ggcggcgtgc gccccgccgc
     9001 gattgcgatt ccacagcgcg ccgtgctgga gggtccgggc accaagatcg tgctgaccgt
     9061 caacgcccag ggcatcgtcg aaccgcgccc cgttcaggta ggcgactggt ccggagagga
     9121 ctggattgtc ctcggcggcc tcaaggccgg agaccaggta atcgtggatg gcgtggtaaa
     9181 ggcccgcccg ggctcgccgg tcaagatcgc acaggcgccc cccgccaatc ccgcccagcc
     9241 cggccaggtc ccggcggcca agccggatgc cgcccagcag cccgccaaac aataaaatca
     9301 aaggggcatt tgcatgttct cgcgcttctt tatcgaccgc ccgatctttg cggccgtcct
     9361 ctcgatattc cttgtgcttg cgggtctggc gtcgatgcgt acgctgccga tcgcgcagta
     9421 tccggagatt gccccgccgg tggtgaccgt gcgcgccgtg tatcccggcg cctccgccga
     9481 ggtgctggag cagacggtcg cggcacccct ggagaaccag atcaacggcg tcgagaacat
     9541 gctctacatg gcctcgaatt cgtcctccaa cggggtggtg gaaatcaacg tcaccttcga
     9601 gatcggcacc aacatcgacc aggcggcgct gaacgtcaat aaccgcgtca aacaggccga
     9661 agcgcggctg cctctggaag tgcgtcgcca gggcgtaacg gtggaaaaga attcctcgtc
     9721 gttcctgcag gtgctggcct tcaattcgcc ggacggcacc ttcgacgacc tgtacgtcag
     9781 caatttcgtc accctgaacg tactcgacac catcaagcgc attccgggca ccaccaacgt
     9841 gcagatcttc ggtgccaagg actatgcgat gcggatctgg ttgcgcccgg accgcatgac
     9901 ccagctcaag ctcaccaccg gcgatctgct gcgggtactg aacgaacaga acgcacagtt
     9961 cgccgccggc aagatcggcc aggcaccgac cggcgggacg caggagatgg tctataccat
    10021 caccaccaag ggccggctcg ctgacgcgcg cgagttcgag aacatcatcg ttcgctcgaa
    10081 cccggacggc tcggcgttgc gcctcaagga cgtggcgcgc gtggaactcg cttccaagga
    10141 ttacgacttc atcggccggg tcaacggcaa ggagtccgcc cttctgggta ttttcctgca
    10201 acccggcgcc aacgcactcg atgtcgccaa ggatgtcggg cggaccatgc aggccctgtc
    10261 caaacgcttt ccggcgggga tggagtacca gatcgtttac gacaccaccc gcttcgtcga
    10321 ggtgtcgatc cgcgaagtgg taaagaccct tgccgaggcg atggcgctgg tatttctggt
    10381 ggtgtacctg ttcctgcaga actggcgcgc caccctgatc ccctttgccg ccgtgccggt
    10441 gtcgctgatc ggcactttcg cgggcctgtt tctccttggc tactcgatca atacgctgac
    10501 cttgttcggc atggtgctgg cgatcggcat cgtggtggac gacgccatcg tggttctgga
    10561 aaacgtcgag cgcatcatgc acgaggagca caagaatgcg cgcgaagcgg cgatcaaggc
    10621 catgagcgag gttaccggcc cggtaattgc cattgtgttg acgctgtgcg cagtgttcgt
    10681 gccgatcgcc ttcctgggcg gtctcactgg cgagctctat cgccagttcg cggtcaccat
    10741 cgccatcgca gtagtgctct ccgggatcgt cgcactcacc ctgacgccga gcttgtgcgt
    10801 cctgatcctc aagcgcgagc acaaggcgcc ggggcgattc ttcagctggt tcaacgactg
    10861 gttccgccgc gtgaccgggc gctataccgg tggtgcgggc tggatcattc gtcgtggcgc
    10921 catcggcatg gttctgttcg ctgtcatggt ggtggtcgcc gccggcctgt ggaaaatcac
    10981 cccgggcagc ctggtgccgg acgaagatca gggttactac atcggcgcgg tgttcctgcc
    11041 ggacggcgcc actctgcaac gcaccgacag ggtcgtgagc gaagtgctca aggcggtgca
    11101 atccaatccc gccaacgagt acgccgtggc cttcaccggc ctggatttca tcggtggcgg
    11161 cttccgcaac agtgccgcga ccatcttcgt gacgcagaag cactgggacg aacgccccgg
    11221 tgtgggcacg cagcagttga tcggcgatta cttcatgaag accgcccaca tcaaggaagg
    11281 cctggtgctg gcgtttgcgc cgccgccgat cttcggcctg ggcaatgccg gtggattcga
    11341 gttctacatc cagaaccgcg gcgagggcgg gccacagcgg ctcgcccagg agatgggcaa
    11401 attcctagcc gccgccaaca aggatccgca gctcgccggt gtgcagactc tgtggcgcgc
    11461 cggcgtgccg cagctgcacg tggacgtgga ccgggagaag gccaaagcat tgggcgtgcc
    11521 gctaaatgat ttgttcgaca cgctggcggc tacgctcggc tcctactacg tcaacgattt
    11581 caacaagttt ggacgcacct ggcaggtgct gatgtccgcc gagccatcct tccgaatgcg
    11641 tcccgattcg atcggcgagc tctacgtgcg ttcggaaaaa aagggaatgg tgccaatcag
    11701 ggcgcttgcc aacgtcagct atacctccgg tccg
//
LOCUS       DASQOP010000214         4599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1508620, whole genome shotgun sequence.
ACCESSION   DASQOP010000214 DASQOP010000000
VERSION     DASQOP010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4599
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1508620"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VE030_08715"
     CDS             complement(<1..386)
                     /locus_tag="VE030_08715"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZF23532.1"
                     /translation="MQFNSNDFGSSLQSEINVTPLVDVVLVLLIIFMVVVPLLMEGYD
                     VDIPRPRASTDTSAQVAESQLILSIGSAACPILGPLAGEGIPKDCQVTLADQKIPAAE
                     LPARVAQILGGRPAEQRALFLAVDDRL"
     gene            complement(391..807)
                     /locus_tag="VE030_08720"
     CDS             complement(391..807)
                     /locus_tag="VE030_08720"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZF23533.1"
                     /translation="MRLGEPSKMQAAINITPLVDVVLVLLIIFMVMAPQMRKGPDVNV
                     PNTTKPNAQGDERGRILVSIDEAGGLWINDRSVAPEHFGEALNAAVGAEPNPKIVIRG
                     DAKLNFGEVRQAMLAIEQAGFRGVGLIAKRSGAEGK"
     gene            complement(794..1531)
                     /locus_tag="VE030_08725"
     CDS             complement(794..1531)
                     /locus_tag="VE030_08725"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HZF23534.1"
                     /translation="MSLTELLRHVGIFGGAVMVCLALLSVFSVGMIIDKQRRFGLAWR
                     QSEKFKPEFKKFLHGGDAQDLIEAGKLHENSYVAQVVSAGIIEYDGVRQRGGDPVASL
                     ELVTSALRDSISEMLIQLKGGLGLLATIGSTAPFIGLFGTVVGIINAFRSIAATGSGG
                     MSVVSGGIAEALVSTALGIFVAIPAVVAFNHFTGRIETFHVAMNRASSQLVNCLFKTP
                     ELKELDVEVDEVKEPRVKKEGPVYAAR"
     gene            complement(1528..2016)
                     /locus_tag="VE030_08730"
     CDS             complement(1528..2016)
                     /locus_tag="VE030_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23535.1"
                     /translation="MKKTSVHWILLGVLALTPAAVLAQQAAQPKTKAAQVQTEKPQAK
                     TEEAKKEEPKKQTEEAQAKTEEDGKLTPVPGGKALGMSILGNQEAPKALVIVPWKSSK
                     LGNGPNVSTLLDDSRLPIDKEVFMRMLNYYEIRSETARPSGATTDGNAAAPANAAQGR
                     KP"
     gene            complement(2013..>4599)
                     /locus_tag="VE030_08735"
     CDS             complement(2013..>4599)
                     /locus_tag="VE030_08735"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZF23536.1"
                     /translation="LGGPETMQEHFSKFGNRSYEDRIYSSLGEHYLGKLRYDDAAKTY
                     KAFVALYPFHRAAPRFSMRVVDTFTQGGFPKLVLESKREFASKYGLKAEYWRHFKPEE
                     SPEVLAYLKTNLKDLATHYHAQYQNANDANEKLTNYREASQWYGAYLESFPREADSAS
                     VNYQLADLLLENKDFGEAAKQYERTAYGYAQHPQSAAAGYAAIYAFRAQLKVADKKQQ
                     DAVKRDTVASSLKFADAFPQDEHAAAVLGAAADDMYEMKDYKAAIEADQRVIDKYPGA
                     GESIRRSAWMVLAHGSFELAEYPQAEHAYTQVLAVTPQGDESRAALVDNLAASIYKQG
                     ELANDAKDYRAAADHFLRIRTAAPTSSIRATAEYDAGAALIRMQDWKAAVDVLEAFRT
                     TFPENKLQLEATKQIAYAYRQNGELSRAASEYDRIASQSHDPALRSEALLDAGDLYAQ
                     SNSRDRALDAYNRYVKEFPKPVETAIETRFKIAEMYKAAHDETLYHQQLEEIVSVDAG
                     AGSERTGRTRTVAARSALVLAEQLYGNFVVVKLRQPFETSLQDKKQRMDATIAALGRL
                     VDYGIDEVTAAATYYIAETYSNFSHSLLDSERPDDLKPQDLEEFKNKLDEAAFPFEEK
                     TIKVHEKNMELLHAGVFNSWTEKSLTRLTELMPGRYAKRETSSGFLAAINSHVEESPL
                     SQVSNGSTPGNAITQGVAVTDEMRAAYESAVGMLKEERYEPGIALLLKMTEKMPALTA
                     AHIDLGIAYARTGDLDRAEASLNKALESGPKQPVAYNELGMVQRRKAQFAKARTSYEA
                     ALAQSADFQYAHRNLAILCDLYLGDYKCATEHYEAYSRIVPDDAEVVKWIADLRNRAK
                     KQEER"
BASE COUNT          815 a         1416 c         1343 g         1025 t
ORIGIN      
        1 aggcgatcgt cgacggccag gaatagcgcg cgctgttccg cgggccggcc gcccagaatc
       61 tgcgccacgc gcgcgggcaa ttcggctgca gggatctttt gatccgccag ggtcacctga
      121 cagtccttgg ggatcccctc ccctgccagc ggcccgagga tggggcacgc cgcggacccg
      181 atgctcagga ttaactggga ttcggcgacc tgagccgacg tatctgtgga ggctcgaggg
      241 cgagggatat cgacgtcgta gccctccatc aggagtggca cgacgaccat aaatatgatc
      301 agcagcacga gcaccacgtc gacgagcggc gtgacgttga tttcagactg cagactggag
      361 ccgaagtcgt tgctgttgaa ctgcatgatg tcactttcct tccgcgccgg agcgcttggc
      421 gatcagcccc accccgcgga agccggcctg ctcgatcgcg agcatggcct gccggacctc
      481 gccgaaattg agcttggcat cgccccggat cacgatcttg gggtttggct cggcgcccac
      541 cgcggcgttg agggcttcgc cgaaatgctc gggtgcgacg gaccgatcgt tgatccacag
      601 cccgccagcc tcgtctatgg agaccaggat tcggcctctc tcgtcgccct gcgcgttcgg
      661 tttcgtggtg ttcggcacgt tgacgtccgg tcccttgcgc atctgcggcg ccatcaccat
      721 gaagatgatc agcagcacca gcaccacgtc gaccagcgga gtgatgttga tggccgcctg
      781 catcttgctg ggttcaccga gccgcataga ccggcccttc ctttttgacc ctcggttcct
      841 tgacttcatc cacttcgacg tcaagctcct tgagctccgg tgttttgaac aaacagttca
      901 cgagctggga cgaagcccga ttcatcgcga catggaaagt ctcaatcctg cccgtgaagt
      961 ggttgaaagc gacgacggca ggaattgcga cgaagatacc cagggccgtt gagaccaggg
     1021 cctcagcgat gccgcccgaa acgacagaca ttccgccgga ccccgtggcc gcgatgctcc
     1081 gaaaagcgtt aatgattccg accacggtgc cgaaaagacc aatgaacggg gcagtggagc
     1141 cgatcgtagc aaggagcccg agacccccct tcaactgaat cagcatctcg gatatggagt
     1201 ccctgagtgc gctcgtgacg agttcgagcg aagcgaccgg atcgcctccc ctctggcgca
     1261 cgccgtcgta ctcgatgatg cccgccgaga ccacttgcgc cacgtaggaa ttctcgtgca
     1321 gcttcccggc ttcgatcagg tcctgggcat ctcccccgtg aaggaacttc ttgaactcgg
     1381 gcttgaactt ctccgactgg cgccaggcca gaccgaagcg gcgctgctta tctatgatca
     1441 tccccaccga aaaaacggaa agcagggcga ggcataccat caccgcgcct ccgaatatac
     1501 cgacgtgtcg taacagttca gtcaaactca tggcttcctc ccttgcgctg cattggcggg
     1561 cgcagccgca tttccgtccg tcgtcgcacc gctggggcgc gccgtttccg acctaatttc
     1621 gtagtaattc agcattcgca tgaatacctc cttgtctatg ggtagcctcg agtcatcgag
     1681 taacgtcgag acgttggggc cattgccgag tttcgagctc ttccagggaa cgatgaccag
     1741 tgctttaggc gcttcctgat tgcccaaaat ggacatgccg agcgcctttc ctcctgggac
     1801 gggcgtcaac ttgccgtctt cttccgtctt tgcctgcgcc tcttcagtct gtttctttgg
     1861 ctcctccttc tttgcctctt ctgtctttgc ctgcggcttt tcggtctgta cctgtgctgc
     1921 tttcgtcttt ggctgcgctg cttgctgcgc gagtacagcc gcgggcgtca aggcaaggac
     1981 cccaagcaaa atccagtgaa cgctagtctt cttcatcgct cctcctgttt ttttgcgcgg
     2041 ttgcgaagat cggcaatcca cttgacgacc tcggcatcgt ccggaacgat ccggctgtat
     2101 gcctcgtaat gctccgtcgc gcacttgtaa tctccgagat acaggtcgca gagaatcgca
     2161 agattcctgt gcgcatattg aaagtcggcg gactgtgcca gggccgcctc ataactcgtg
     2221 cgcgccttcg cgaactgtgc cttgcgccgc tgcaccatac cgagttcgtt ataggcgaca
     2281 ggctgttttg gacccgattc cagcgccttg ttcaggctgg cctcggcgcg gtccaggtcg
     2341 ccagtgcgcg cgtatgcaat gccgagatcg atgtgcgctg ccgtcaatgc aggcatcttc
     2401 tcggtcattt tgagcagcaa agcaatgccc ggttcgtacc gctcctcctt cagcatgccc
     2461 actgccgatt cgtaggccgc acgcatttca tccgtgacgg ccacgccctg cgtaatcgcg
     2521 ttgcccggcg tcgagccgtt ggaaacctgc gacagcggcg attcctccac gtgactgttg
     2581 atcgcggcga ggaagccgct gctcgtttcg cgtttcgcgt agcgacccgg catcagctcg
     2641 gtgagcctgg tgagactctt ctcggtccac gagttgaaga cgccggcgtg caataactcc
     2701 atgttcttct cgtgtacttt gatggttttt tcctcgaacg gaaacgctgc ctcatcgagc
     2761 ttgttcttga actcctccag atcctgtggt ttcaagtcgt ccggccgctc cgaatcgagc
     2821 agcgagtgac tgaaattgga gtaggtctcg gcgatgtaat aggtcgcggc cgccgtcacc
     2881 tcgtcgattc cgtaatccac cagccggccc agggccgcaa tcgtggcatc catgcgctgc
     2941 ttcttatcct ggaggctggt ctcgaagggt tgccgcagct tcacgacgac gaaattccca
     3001 tagagctgct ctgcgagaac gagagccgaa cgtgccgcaa ccgtgcgagt ccggccggtc
     3061 ctttccgatc ctgcgcccgc gtcgacgctt acgatttctt ccaactgctg gtgatagaga
     3121 gtttcgtcat gcgcggcctt gtacatctcg gcgatcttga accgggtctc gatggccgtt
     3181 tctacgggtt ttggaaactc tttaacgtac cggttgtatg catccagggc gcgatcccgg
     3241 gaattggatt gcgcgtagag gtctcccgca tcgagcagcg cctcgctgcg taacgccgga
     3301 tcgtgggact gggaagcgat gcggtcgtat tcgctggcgg cgcgcgataa ctcaccgttc
     3361 tgccggtagg cataagcgat ctgtttggtg gcctcgagct gcagtttgtt ttccgggaag
     3421 gtagtacgaa acgcttcaag cacgtccacc gccgccttcc agtcctgcat gcggattagc
     3481 gcggcgcccg cgtcgtactc agccgtcgca cggatagagg atgtaggcgc cgccgtgcga
     3541 atcctgagga agtggtctgc cgccgcgcga taatcctttg cgtcgtttgc gagttcaccc
     3601 tgtttgtaaa tcgaggcggc aaggttgtcg acgagcgcgg cgcgcgactc gtcgccctgc
     3661 ggcgtgaccg ccagcacctg ggtgtaggcg tgctcggcct gcgggtactc ggcgagctcg
     3721 aacgaaccat gcgcaagcac catccacgcg gaacgccgga tcgactcccc tgcgcccgga
     3781 tacttatcga tgacgcgctg gtcggcctcg atggcagcct tatagtcctt catctcgtac
     3841 atgtcatcgg cggccgctcc gaggaccgcc gcggcgtgtt cgtcctgcgg gaacgcatcg
     3901 gcaaatttga gcgagcttgc gactgtatcg cgcttgacgg cgtcctgctg cttcttgtcg
     3961 gcaaccttca gctgcgcgcg gaatgcgtag atcgcggcgt atcctgcagc tgccgactgc
     4021 ggatgctgcg cgtatccata agccgtgcgc tcatactgtt tggccgcctc gccgaaatcc
     4081 ttgttctcca aaagcaggtc cgcaagctga tagttgacgg atgccgaatc agcctccctc
     4141 ggaaaggact cgaggtaggc gccataccat tggctggcct cgcgataatt cgtgagcttc
     4201 tcgtttgcgt cgtttgcgtt ctgatactgg gcgtgataat gcgtcgcgag gtctttcagg
     4261 ttcgtcttca ggtaggccag aacttccggc gactcttccg gtttgaaatg tcgccagtat
     4321 tcggccttca ggccgtactt ggaagcgaac tcccgcttcg actccagtac cagcttgggg
     4381 aacccgccct gggtgaaagt atccacgacg cgcatgctga agcgcggcgc ggcgcggtgg
     4441 aacggataaa gcgctacgaa tgctttgtac gtcttggccg catcgtcgta gcgcagcttt
     4501 ccaagatagt gctcgccaag actggagtaa atgcggtcct cataggagcg attcccgaac
     4561 ttggagaagt gctcctgcat ggtttccggc ccgcctagg
//
LOCUS       DASQOP010000215         9123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_502026, whole genome shotgun sequence.
ACCESSION   DASQOP010000215 DASQOP010000000
VERSION     DASQOP010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9123
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502026"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="VE030_08740"
     CDS             <1..861
                     /locus_tag="VE030_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HZF23537.1"
                     /translation="LAGLYGIAIAATAMLSMTGMVVALDAYGPITDNAGGIAEMAGLP
                     SKIRDITDPLDAVGNTTKAVTKGYAIGSAGLAALVLFADYTHNLEAQGKLVAFSLSDP
                     AVIIGLFLGGLVPYLFGAMALEAVGRAAGSVVNEVRRQFREIKGIMEGTAKPDYSRAV
                     DMLTKAAIKEMIVPSLLPVLVPVVVAFGMNWLMGPGAGVKALGGLLIGTIVTGLFVAI
                     SMTTGGGAWDNAKKYIEDGHYGGKGSDAHKAAVTGDTVGDPYKDTAGPAVNPLIKIIN
                     IVALLLVPVL"
     gene            complement(879..1274)
                     /locus_tag="VE030_08745"
     CDS             complement(879..1274)
                     /locus_tag="VE030_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23538.1"
                     /translation="MRSTIRILLAITLSLGIAPIEVRGEEPGATTSPQLAKLRKEITD
                     LVGPARCMNLVQCRVAAIGIDSCGGPAGYLVYSWLSTDKGALETKIAEYDFLQEDLQK
                     KQQPAASCSALPEPTAVCVNGRCVLPGGR"
     gene            1361..1915
                     /locus_tag="VE030_08750"
     CDS             1361..1915
                     /locus_tag="VE030_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006713141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HZF23539.1"
                     /translation="MALKATIFKAELQIADMDRRYYGDHTLTIARHPSETDERMMVRL
                     LAFAMHADGALSFGRGLSTDDEPDLWQKDLTGTIEIWIDVGLPDEKRVRRACGRARQV
                     FVYSYGGRGAELWWDQVNAKLGRVENLSVISLPAASTQALAALAFRNMQLQFTIQEGQ
                     IWISDKDKVVEVGPATLKAPAPQR"
     gene            1999..2823
                     /locus_tag="VE030_08755"
     CDS             1999..2823
                     /locus_tag="VE030_08755"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HZF23540.1"
                     /translation="MESAVVSSLEALAAAIPDGALLAVPKDSSGVAMAATRELVRRGV
                     RDLRLVCVPVGGLQADILIGAGAVATIETSAVTLGEFGGAPRFAAAVREGAIRVLDAT
                     CPAIYAALQAGEKGIPFMPLRGLIGTDLLVHRQDWKVIDNPFAGQDRIVALPGIRPDI
                     SLFHAPLADRHGNVFVGAKRELMLMAHAARQTLVTVEEITDDNLLDDPQRSAGVLPAI
                     YVSRVAAAKHGAWPLAFWDLYPDDEAALANYVAAAGTAAGFRHFVDSWLDTRRAVA"
     gene            2820..3551
                     /locus_tag="VE030_08760"
     CDS             2820..3551
                     /locus_tag="VE030_08760"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HZF23541.1"
                     /translation="MSEFSRTELLITVIARLLRGCRHVAVGASSPIPGAGALLARTRA
                     REQMRVTVLGSLRHEGFTSGGVELFDMAAQGRIDAFFLGGGQIDGQANINLVGTGEYP
                     NNEVRWPGSFGSAYLYFLVPRVILFREEHSRRVMVPEVDFISAPGVSEPGVHRLGGPY
                     AMLTELGLFSFHRERRRFRLESVHPGRSVEEIRDNTGFEFDCPDRVPITPRPEPETLS
                     AIRSAIRSDIAETYPRFAARLAAAG"
     gene            complement(3568..3918)
                     /locus_tag="VE030_08765"
     CDS             complement(3568..3918)
                     /locus_tag="VE030_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23542.1"
                     /translation="MLPPAYIGRDGMCREEDRAMVAANGPARAIDGIMEVEKKIIRLA
                     EERNIHGLRFEWNDDLDFGHLLDPVPLNVFAAGGKSAEAEFSQAELAQVVAGPDAATA
                     TKIDRIVQAIATEA"
     gene            complement(4082..5179)
                     /locus_tag="VE030_08770"
     CDS             complement(4082..5179)
                     /locus_tag="VE030_08770"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF006707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HZF23543.1"
                     /translation="MSSVIAARTTGGTDIAALLGADADKLLSHVCKGIPKELLHLPGP
                     DFVDRIVAQTDRPGPVLRSLQQMFGHGRLAGTGYLSILPVDQGIEHSAGASFAKNPAY
                     FDPENIVKLAIEGGCNAVASTLGVLGSCSRKYAHKIPFLLKFNHNEFLTYPNKYDQIF
                     FAGVKQARDMGAVAVGATIYFGSDESARQIVEVSKAFEIAHEMGMATVLWCYLRNPAF
                     KSDKDYHVSADLTGQANHLGVTIEADIIKQKLPENNGGYNALNTKENPYGKIDKRVYT
                     DLTSDHPVDLTRYQVANCYMGRAGLINSGGASSGESDLAEAVRTAVINKRAGGMGLIS
                     GRKAFQRPMKEGVGILNAIQDVYLSKQVTVA"
     gene            5299..5823
                     /locus_tag="VE030_08775"
     CDS             5299..5823
                     /locus_tag="VE030_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HZF23544.1"
                     /translation="MIHSLGDRKVITRGDHWVAHNATVIGSVVLEKEVSIWFNAVVRG
                     DNDLITIGEGSNIQDHSVLHTDEGVKLTIGRNVTVGHHVMLHGCTIGDGSLIGIKSVI
                     LNNARIGKHCLIGANALITEGKEIPDRSLVVGAPGKVVRQLTDEEVARLLWNAEHYVN
                     NYKRYQRDFREQGA"
     gene            6051..6278
                     /locus_tag="VE030_08780"
     CDS             6051..6278
                     /locus_tag="VE030_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23545.1"
                     /translation="MMKFGLKIRTRSGLPVDNLVVHARDQAEAERKITQMYMHCEILE
                     CRQIQTATRDDATDLDGIISLISRQGDEEKT"
     gene            complement(6305..7078)
                     /locus_tag="VE030_08785"
     CDS             complement(6305..7078)
                     /locus_tag="VE030_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HZF23546.1"
                     /translation="MAKIAVFNQKGGVGKTTTSLNLTAALARRGYNPLSIDLDPQAHL
                     SYVCGSVVGNADDSVFGFYQNAKPLTQLIRPALGGWLVIPAHVELSKVDTQFGKGPNI
                     LNRLNTGIVKENLNTGRPIVLDCCPMLGVLSLSAIFASDRVLVPVSADYLAVQGVNQV
                     EKTFRALEHVLKKRVLRRYVITRFDGRRKMSHQIFEELKARHGDEVCDTRISENVSVA
                     ESPASKKDVFAHSPDSRGAQDYEALLNELVDSGFIEARN"
     gene            complement(7175..8677)
                     /gene="glpK"
                     /locus_tag="VE030_08790"
     CDS             complement(7175..8677)
                     /gene="glpK"
                     /locus_tag="VE030_08790"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014896124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HZF23547.1"
                     /translation="MSAILALDQGTTSSRAIVFDEAGQVLGTAQQELTQIYPQSGWVE
                     QDAEEIWSTQLATARAALAQAGLNAGNIAAIGITNQRETTVVWERATGRPIANAIVWQ
                     DRRTAGLCEQLRSQGVEALFTGRTGLLLDAYFSGTKVRWLLDHVEGARTRAENGALVF
                     GTVDTWLIWKLTGGRVHATDVSNASRTMMFNIHTRDWDDKLLGLLRVPRALLPKVVAS
                     SAVCGECDSAHFGRGLQIAGIAGDQQAALFGQQCTRSGMVKNTYGTGCFMLMHTGARP
                     VDSHNRLLTTVAWKIGETIEYALEGSVFIAGAAVQWLRDGLGIIRTSGEVEALARSVP
                     DTGGVYLVPAFAGLGAPYWDPHARGTIVGMSRGTTAAHVARATLESIAYQTADVLEAM
                     HADSGIAVTELRVDGGATQNDLLMQFQADVLEVPVVRPKIYETTALGAAYLAGLAVGF
                     WKNADALAAQWQLDRRFEPAMSAACRAALKAGWIKAVKRSRAWAEAAEGD"
     gene            8768..>9123
                     /locus_tag="VE030_08795"
     CDS             8768..>9123
                     /locus_tag="VE030_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HZF23548.1"
                     /translation="MSNDLFLTALARMGLLQAGERPAITPLTGGVSSDIVRVDLAAGP
                     ICVKRALAKLKVEADWRAPVERNRYEVQWMQVAAGIIPAAVPRILGEDRETGMFAMQF
                     LDSATYPVWKAQLRDG"
BASE COUNT         1657 a         2919 c         2854 g         1693 t
ORIGIN      
        1 ctggccggcc tgtatggcat tgccattgcc gccaccgcga tgctgtcgat gaccggcatg
       61 gtcgtggcgc tggacgccta cggtccgatc accgacaacg ccggcggcat cgccgagatg
      121 gccgggctgc cgtcgaagat ccgcgacatc accgatccgc tcgacgcggt gggtaacacc
      181 accaaggcgg tgaccaaggg ctacgccatc ggctccgccg gcctggccgc gctggtcctt
      241 tttgccgact acacccacaa cctcgaagcc cagggcaagc tggtggcatt cagcctgtcc
      301 gatccggcgg tgatcatcgg gctctttctc ggcggcctgg tgccctatct gtttggcgcg
      361 atggcgctgg aagcggtggg ccgggctgcc ggttcggtcg tgaacgaagt gcgccgccag
      421 tttcgcgaga tcaagggcat catggaaggc accgccaagc cggattattc gcgcgccgtg
      481 gacatgctga ccaaggccgc gatcaaggaa atgatcgtgc cgtcgctgct gccggtgctg
      541 gttccggtgg tcgttgcctt cggcatgaac tggctgatgg gcccgggtgc gggcgtcaag
      601 gcgctcgggg gtcttctgat cggcaccatc gtcaccggat tgttcgtggc gatctcgatg
      661 accactggcg ggggcgcatg ggacaacgcc aagaagtaca tcgaggacgg acattacggc
      721 ggcaagggtt ccgacgcgca caaggcggcg gtgaccggcg acaccgtcgg cgatccctac
      781 aaggataccg ccggtcccgc agtgaacccg ctgatcaaga taatcaacat cgtcgcgctg
      841 ctgctggtgc cggtgctgta gaagcaccag atttcgcgct agcggccgcc gggaagcacg
      901 cagcggccat tgacgcacac ggcggtcggc tcgggaagcg ccgaacagct tgctgccggc
      961 tgttgcttct tctgcaggtc ctcctgcagg aagtcgtatt ccgcgatctt ggtctcgagc
     1021 gcgcccttgt cagtcgagag ccacgaataa accaggtacc cggccggtcc gccgcaggaa
     1081 tcgattccaa ttgcggcgac acggcattgc accaggttca tgcagcgcgc aggcccaacc
     1141 aggtcggtaa tttccttgcg tagtttcgcg agctgcggcg atgtcgttgc gccgggctct
     1201 tcgccccgta cttcgatggg cgcaattccc agtgacaggg tgatcgccag caggatgcgt
     1261 atcgtcgagc gcatgtcgtc cccaagccaa gccggtcatt tcaactattt cacgtgccgg
     1321 ggtgacaatg caagcgcacg aaaaaggcat ccctgacccc atggccctca aagcaacaat
     1381 cttcaaggcg gaactgcaga tcgccgacat ggaccgtcgc tactacggcg accatacgct
     1441 gaccatcgcc cgccatccgt cggagaccga cgagcgcatg atggtgcggt tgctggcgtt
     1501 cgcaatgcac gccgatgggg ccttgtcgtt tggccgagga ttgagcaccg atgacgaacc
     1561 cgacctctgg cagaaggacc tgaccggcac catcgaaata tggatcgatg tcggtctgcc
     1621 cgatgagaaa cgcgttcgca gggcctgcgg tcgcgcccgg caggtgttcg tctacagcta
     1681 cggcggacgc ggcgccgagc tctggtggga tcaggtcaac gcgaaactcg ggcgggtgga
     1741 gaatctgtcc gtgatcagcc tgcctgcggc ctcaacccag gcgttggcgg cgctggcgtt
     1801 tcgtaacatg cagctgcaat tcaccatcca ggaaggccag atctggatat ccgacaagga
     1861 taaagtcgtc gaagtcggtc ccgcgacttt gaaagcgccg gcgccacaac gctgacgaag
     1921 agcggcttcc gcgtcataat gccctgccgg taacgctgga cgcagttgcg atttgccggg
     1981 gatccgcagg agccgaattt ggaatcagcc gtcgtgtcgt cgctcgaagc gctcgccgcc
     2041 gccatacccg atggcgcact gcttgccgtg cccaaggact cgtcgggcgt tgccatggct
     2101 gcaacgcgtg aacttgtgcg ccgcggtgtt cgcgacctgc gcctggtgtg cgtcccggtc
     2161 ggcgggctgc aagccgatat cctgatcggc gccggcgcag tcgcgaccat cgaaacttcc
     2221 gcggtaacac tgggtgaatt cggcggcgcg ccgcgcttcg ccgccgcggt acgcgaaggc
     2281 gcgatccgcg ttctcgacgc gacctgtccg gcgatctatg cggcgctgca ggccggtgag
     2341 aagggcattc cgttcatgcc gctgcgcggc ctcatcggca ccgacctgct ggttcaccgg
     2401 caggactgga aagtgatcga caatccgttc gccgggcagg accggatcgt cgcgcttccc
     2461 ggcatccgcc cggacatatc gctgtttcac gcgccgcttg ccgatcgtca cggcaacgtg
     2521 ttcgtcggcg ccaaacgtga actgatgctg atggcccacg ccgcaaggca aaccctggtg
     2581 acggtcgagg agatcaccga cgacaacctg ctggacgatc cgcaacggtc tgccggcgtg
     2641 ttgcctgcga tctacgtctc ccgggtggca gcggccaagc acggagcgtg gccgctggca
     2701 ttctgggatc tgtatccgga tgacgaggcg gccctggcga attatgtcgc ggccgccggc
     2761 acggccgcag gattcaggca ctttgtcgat tcctggctcg acacccgccg cgcggttgca
     2821 tgagcgaatt cagccgcacc gaattgctga tcacggtgat cgcgcggctg ttgcgcggct
     2881 gccgccacgt cgcagtcggc gcgtcctccc cgatcccggg cgcaggcgcg ctgctggcgc
     2941 gaacgcgggc gcgcgagcag atgcgggtca ccgtgctcgg ctcgctgcgc cacgaaggct
     3001 tcaccagcgg cggcgtcgag ttgttcgaca tggccgcaca gggccgcatc gacgcattct
     3061 tcctcggcgg cggccagatc gacggacagg ccaacatcaa cctggtcggt accggcgagt
     3121 atccgaacaa cgaagtgcgc tggcccggct ccttcggctc tgcttatttg tactttctgg
     3181 tgccgcgcgt gattctcttc cgcgaagagc acagccgccg cgtcatggtg ccggaagtgg
     3241 acttcatcag cgcgccgggc gtgagcgaac ccggcgtgca tcgccttggc ggtccgtacg
     3301 cgatgctgac cgaactggga ttattttcct tccaccgcga gcgccgccgc ttcaggctcg
     3361 aaagcgtgca ccccggccgc agcgtggaag aaatccgcga caacaccggc ttcgaattcg
     3421 attgcccgga tcgggtgccc atcacgccgc gtcccgagcc cgaaaccctg tccgcgattc
     3481 gcagtgccat ccgcagcgac atcgccgaaa cttacccgcg cttcgccgcc cgccttgcgg
     3541 ccgcgggctg acgcgagcgt atccgactca ggcttcggtt gcgatagcct ggacgattcg
     3601 atcgatcttg gtagcggtgg ccgcgtccgg ccccgcgaca acctgcgcga gttcggcttg
     3661 agaaaactcg gcctcggcgg atttgcctcc ggctgcaaaa acgttcagcg gcaccgggtc
     3721 cagcaagtgt ccgaaatcca ggtcgtcgtt ccattcgaaa cgcaggccat ggatattcct
     3781 ctcctctgcc aaccggatga tctttttctc aacttccatg atgccgtcta tggccctcgc
     3841 cgggccgttc gccgcaacca tcgcccgatc ctcctcgcgg cacataccgt ctcttccaat
     3901 ataggccggc ggcagcactt taaaagaagt ttgtgacggc gtccgcctcc cggattccgg
     3961 tccggcggca gccggcggaa cgcgaatcgc aaagcaggat ggtcactcgg gagcatgaag
     4021 cgcaacgggg tgtgccgcaa acggcacacc ccgtctctga aattgctctg cgacagatgg
     4081 tttaggcgac ggtcacctgt ttcgacaggt acacgtcctg aatcgcgttc aggatgccaa
     4141 cgccctcctt catcggccgc tggaaggcct tgcggccgga gatcaggccc atgccgccgg
     4201 cgcgcttgtt gatcactgcg gtgcgtaccg cttccgcgag atcgctttcg ccggaagaag
     4261 ccccgccgga attgatcagc cccgcgcggc ccatgtagca gttcgccacc tggtagcgcg
     4321 tcaggtcgac cgggtggtcg gaggtcagat cggtgtacac ccgcttgtcg atcttgccgt
     4381 acgggttttc cttggtattg agcgcgttgt agccgccgtt attttccggc agcttctgct
     4441 tgatgatgtc ggcctcgatg gtcacgccga ggtgattggc ctgaccggtc aggtcggcgg
     4501 aaacgtggta atccttgtcg gatttgaatg ccggattgcg caggtagcac cacagcacgg
     4561 tggccattcc catttcatgc gcgatctcga aggccttgct cacctcgacg atctgccgtg
     4621 ccgattcgtc ggagccgaaa tagatcgtcg cgcctactgc gaccgccccc atgtcgcgcg
     4681 cctgcttgac gccggcaaag aaaatctgat cgtacttgtt tggataggta aggaattcgt
     4741 tgtgattgaa cttgagcagg aaggggatct tgtgcgcgta cttcctggaa cacgagccga
     4801 gcacgcccag ggtcgaggcc acggcgttgc agccgccctc gatggcaagc ttgacgatgt
     4861 tctccggatc gaaataggcc gggttcttgg cgaacgacgc accagcggag tgctcgatgc
     4921 cctggtctac cggcaggatc gacagatagc cggttccagc cagccgcccg tgcccgaaca
     4981 tctgctgcag gctgcgcagc acggggccgg ggcggtcggt ctgcgccact atacggtcca
     5041 cgaagtccgg tcccggcagg tgcagcaatt ccttggggat gcccttgcat acgtggctaa
     5101 gcagcttgtc cgcgtctgct cccagcaatg cggcgatatc ggtgccgcct gtcgttctgg
     5161 ctgcgataac tgatgacatg accttggatc ctccctgaac ctgttgaccg gattatcccc
     5221 cttgcggctg gcgggcgtaa acgtgccgct ataattgagc cctttcccag ccttaaccct
     5281 gaccgaaatc aaagaagcat gattcattcc ctgggcgaca gaaaagtgat cacccgaggc
     5341 gaccactggg tcgcgcacaa cgccaccgtg atcggctcgg tagtgctcga aaaggaggtg
     5401 agcatctggt tcaacgccgt ggtgcgcggc gacaacgacc tgatcaccat cggcgaaggc
     5461 tccaacatcc aggaccattc ggtgctgcat accgacgagg gcgtcaagct caccatcggc
     5521 aggaacgtca ccgtcgggca ccacgtgatg ctgcacggct gcaccatcgg cgacggcagc
     5581 ctgatcggca tcaagtcggt gatcctcaac aacgcccgca tcggcaagca ctgcctgatc
     5641 ggcgccaatg ccctgatcac cgaaggcaag gaaatccccg accgctcgct ggtggtcggc
     5701 gcgccgggga aggtggtgcg gcaactgacc gacgaggaag tcgcgcgcct gctctggaac
     5761 gccgagcact acgtcaacaa ctacaagcgc taccagcggg acttcaggga acaaggcgct
     5821 tagcccccct ctccccttct ctctccccac acaaccaggt ggagagggtg atggaaacgc
     5881 gttgattcac gatcgtgtag ttttcggcac cctctcccgc ttgctggaga gcgcgaaggg
     5941 tgagttgatg ttctctgcgt cctctgtggc aaaaaacctt tgcaccaatt gagcccgcga
     6001 gccgggatag cgtctagaat gaccggcgta tgccactagc caatgccccg atgatgaagt
     6061 ttgggttgaa gatccgcacc cgcagcgggc tgccggtaga caacctggtg gtgcacgcgc
     6121 gcgatcaggc cgaggccgag cgcaagatca cgcagatgta catgcactgc gagatcctgg
     6181 agtgcaggca gattcagacg gcgacaaggg acgacgcaac cgatctcgac ggaatcatct
     6241 cgctcatctc ccggcaggga gacgaggaaa aaacctgagc gcggcgaaag cgaccgcccc
     6301 gagctcagtt tcgggcttcg ataaaaccgg aatccacgag ttcgttcagc agcgcttcgt
     6361 aatcctgggc accgcggctg tcgggggagt gtgcgaacac atctttcttg gaggcggggc
     6421 tctccgccac gctgacgttc tccgaaatcc tggtatcgca gacttcgtcg ccgtgacggg
     6481 ccttgagctc ttcgaagatc tggtgtgaca tcttgcgccg gccgtcgaag cgggtgatca
     6541 cgtaacgcct cagcacgcgc tttttcagta cgtgctcgag cgccctgaac gttttttcta
     6601 cttgattgac gccctgcacc gccagatagt ctgccgagac cggcaccagc acgcggtcgg
     6661 acgcgaagat cgcgctcaag ctgagcacgc ccagcatcgg gcagcaatcg agcacgattg
     6721 gccgtccggt attgaggttc tccttgacga tgccggtgtt gaggcgattc aggatgttcg
     6781 ggcccttgcc gaactgcgta tccaccttcg agagttccac gtgggccggg atcaccagcc
     6841 agccgcccag cgccggacga atcaactggg tgagcggctt ggcgttctgg tagaaaccga
     6901 ataccgaatc gtcggcgttg ccgacgacgc tgccgcaaac atacgaaagg tgcgcctgcg
     6961 ggtcgaggtc gatggacagc gggttgtagc cacggcgggc cagggcggcc gtaaggttga
     7021 gcgaagtcgt ggtcttgccg accccaccct tttgattgaa aacggcaatt tttgccatgg
     7081 ctgcccgacg ctgcgccgca ggcctggacg gccgaagcga ctgcttacaa tactaataaa
     7141 atcagtcgct tgtcaataac tttgaaactt tttttcagtc cccttcggcg gcctcggccc
     7201 aggcgcgcga gcgctttact gccttgatcc aacccgcctt gagcgcagcc ctgcacgcgg
     7261 cggacatcgc cggctcgaaa cgtcgatcca gttgccattg cgccgccagg gcatcggcat
     7321 ttttccagaa tcccaccgcc aggccggcca gataggcggc gcccagcgcg gttgtttcgt
     7381 agattttcgg ccgcaccacc ggcacctcca gcacgtcggc ctggaactgc atcaacaggt
     7441 cgttctgcgt cgcgccgccg tccacccgca gttcggtgac cgcgatgccg gagtcggcgt
     7501 gcatcgcctc cagcacatcc gcagtctggt aggcgatgga ttcgagcgtg gcgcgcgcca
     7561 cgtgcgcggc ggtggtgccg cgggacatgc cgacgatggt gccgcgcgca tgcggatccc
     7621 aatacggcgc acccaggccg gcgaacgccg gcacgagata aacgccgccg gtatccggca
     7681 ccgatcgcgc gagcgcctcg acttccccgg aagttcggat gatccccagc ccgtcgcgca
     7741 gccattgcac cgccgcaccg gcgatgaaca cgctgccttc cagcgcgtac tcgatggttt
     7801 cgccgatttt ccaggccacg gtggtcagca gccggttatg cgaatccacc gggcgagcgc
     7861 cggtatgcat cagcatgaag caaccggtgc cataagtgtt cttcaccatc cccgaacggg
     7921 tgcactgctg gccgaaaagc gcggcctgct ggtccccggc aatgccggcg atctggaggc
     7981 cacgaccgaa atgcgcggaa tcgcattcgc cacagactgc gctcgacgcg acgaccttcg
     8041 gcaacagcgc gcgcggcacg cgcagcaggc ccagcagctt gtcgtcccag tcgcgcgtgt
     8101 ggatgttgaa catcatggtg cgcgaggcat tgctgacgtc ggtggcgtgg acgcgtcctc
     8161 cggtgagctt ccagatcagc caggtatcca ccgttccgaa aaccagagcg ccgttctcgg
     8221 cgcgcgtgcg cgcgccctcc acgtggtcga gcagccagcg cactttggtt ccggagaaat
     8281 aggcatcgag cagaagcccc gtgcgcccgg tgaacaacgc ttccacgccc tgcgagcgca
     8341 gttgctcgca caggccggcg gtgcggcggt cctgccagac gatggcgttg gcgatcggcc
     8401 gcccggtggc gcgctcccac accaccgtgg tttcgcgctg gttggtgatg ccgatcgcag
     8461 cgatattccc ggcattcagg ccggcctgtg ccagagccgc tcgcgcagtg gctaactggg
     8521 ttgaccagat ttcttcggcg tcctgttcca cccagccgga ctgcggataa atctgcgtta
     8581 gttcctgctg cgcggtgccg agtacttgcc cggcttcatc gaatacgatc gcgcgtgagc
     8641 tggtcgtgcc ctgatccagt gccaggatgg cgctcatggc tgtgataaag ttcgcatctc
     8701 atttcccacg tatacatgat gcgccaacag cgcggcatcg gccaagcgcg ggaacctcga
     8761 agaaaaaatg agcaacgatc tcttcctcac tgcgctcgcg cgcatggggt tgctgcaagc
     8821 cggggaacgc cccgccatca cgccgcttac cggcggcgta tcttcggaca tcgtccgcgt
     8881 ggacctcgcc gccggaccga tctgcgtgaa gcgcgcattg gcgaaactca aggtcgaagc
     8941 cgactggcgg gcaccggtcg agcgcaatcg ctacgaagtg caatggatgc aggtcgcggc
     9001 gggcatcatc cccgccgcgg tgccgcgcat cctcggcgag gatcgggaaa ccggaatgtt
     9061 cgcaatgcag ttcctggatt ccgcgactta tccggtgtgg aaggcacaac tgcgcgacgg
     9121 cga
//
LOCUS       DASQOP010000216        13579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2520666, whole genome shotgun sequence.
ACCESSION   DASQOP010000216 DASQOP010000000
VERSION     DASQOP010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13579
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2520666"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VE030_08800"
     CDS             complement(<1..335)
                     /locus_tag="VE030_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HZF23549.1"
                     /translation="MNSVKCSLYPAVLITVFVGFTGTTPADLLLTYPLRIRNHEIRAE
                     VANTEESRRRGLMFRDSLGDSSGMVFSYLEAGVNAMWMKNTRIPLSVAFIDRDGRILN
                     IADMEPFSE"
     gene            832..2583
                     /locus_tag="VE030_08805"
     CDS             832..2583
                     /locus_tag="VE030_08805"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HZF23550.1"
                     /translation="MVKQSLHFKRAAVSLAAIAAFGASGVVSAQGVDVLESKLYPNYV
                     TDDMLLNADKDAQNWLHYGRDYQTTRYSPLSQINRDNVKKLRPAWNLSFGVLEGQDSQ
                     AVAVNGMIYVTTSFNKVIAVDAVSGKTQWKYERELPGDVFPKLCCDVVNRGVAVYKNK
                     VYLATLDAHIVALDNTTGKVVWDKKMGDYTYAETFTIMPMALRGKIIFGTAGAEYGVR
                     GWLAAIDADTGAPVWKTYTIPGPGEPGNDTWAGESWKYGGGSTWITGAYDKETNTLYW
                     PVGNPGPDFDRHVRLGDNLFTNSNLVLDPDTGKIRSYFNYTPNDPYDYDGVNEMILVD
                     VGGKKLWLHGSRNGHLYGIDRVNTAKGKAGNEHKCVWVSALQRVNWTKPITPANNCKP
                     IYNYPEKDVVYDKVTTDIAPSLDGGKEWHPMAYSHRTKMVYVPVYDFAMDLQAKKMEW
                     KRGEWYLGAKVITFNAGAGAIKGYDAATGKLGWQKSQSYPATSGILATAGGLVFYGDP
                     EGNVNAVNDETGEHLWSFQVGSGVHGNPTSFTGPDGKQYVAVVYGAGGGGIWPLFYGE
                     YLKKNQKGGGLMVFTVE"
     gene            2783..3625
                     /locus_tag="VE030_08810"
     CDS             2783..3625
                     /locus_tag="VE030_08810"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZF23551.1"
                     /translation="MRKLLTLSGLALLLILAAVVARAQVADPDEISDTLERSKAKGKL
                     IACADPYNFPYAAQNIDPPGFDIEIIRDIAKRGGMSLEMYWADTGTRGGTSRAFRNSI
                     LKKRCDVFLGLSDSGDDDMLMGQLAFTKPYLGLGYVLVVQGKAEGMKSIAELKKANIK
                     VGVPMSTPIDDYLFTNGVPRDLFLDNRRIMESMAKGELDASLVWATAIAVARREYPKV
                     TFRMVDGYVPEAEQRWNLNFVVRKQDKALMEFINGGIAELLSNGRMKQIVESYGVPFY
                     PPFS"
     gene            3652..4137
                     /locus_tag="VE030_08815"
     CDS             3652..4137
                     /locus_tag="VE030_08815"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZF23552.1"
                     /translation="MVGSKLKAFLGQWAALAVVALALGLGTAVQPAAAQHKANENDME
                     VPTNNMEIVQQGRAKYAQRCSFCHGGDGHGGKGPCLTCGVFKYTGNTNSEIYVTIAVG
                     VPRSLGGTMGAFGTTMSQEEILSVITFLRWEEKRRIAAGEVPDPKLNKGNELPVFPTT
                     N"
     gene            complement(4228..4677)
                     /gene="dut"
                     /locus_tag="VE030_08820"
     CDS             complement(4228..4677)
                     /gene="dut"
                     /locus_tag="VE030_08820"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HZF23553.1"
                     /translation="MHTIDVRILDVRLRDQLPAYATAGSAGLDLRACIDAPIAFTPGQ
                     TELIPSGLAIHLDDPGLAAVVLPRSGLGHKHGIVLGNLVGLIDSDYQGQIFVSVWNRG
                     SNAFTLNPMERIAQLVVVPVVQVGFKVVEQFAASVRGAGGFGSTGKH"
     gene            complement(4707..5915)
                     /gene="coaBC"
                     /locus_tag="VE030_08825"
     CDS             complement(4707..5915)
                     /gene="coaBC"
                     /locus_tag="VE030_08825"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HZF23554.1"
                     /translation="MTELSNKRILLGVTGGIAAYKSAELLRELQRQGADVQVIMTESA
                     CRFVTPVTMQALSGKPVFTDMWDSRTPNGMPHIELSRDRDIIVVAPATAGFIAKLVHG
                     AADDLLSTLCLARECPLMVAPAMNRQMWENPATQRNIEQLHRDGLVVLGPDSGDQACG
                     ETGMGRMLEPADLARSIGAWFQPKLLAGTHVLITAGPTFEPIDPVRGITNRSSGKMGY
                     AVARAALEAGAQVTLISGPVSLEAPASTHLVRVETARQMLDAVNAHVVRCDIFVGVAA
                     VADYHVSNNKPQKIKKDGAIPTLELVPNPDIIEEVARRPNPPFCVGFAAESENLEQNA
                     EAKRRRKKLPLLAANLAQAAIGADESELVLFDDDGVHRMVRASKIELARALIRHIAKL
                     YRPRKTPVTA"
     gene            5994..6668
                     /gene="radC"
                     /locus_tag="VE030_08830"
     CDS             5994..6668
                     /gene="radC"
                     /locus_tag="VE030_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HZF23555.1"
                     /translation="MAITDWPADDRPREKLLAKGPGGLSDAELLAIFLRTGVKGKSAV
                     DLARELLVRFGSLGELVAASHESFCSVPGLGDAKYVQLQAAMEMARRALVEDMKGGIN
                     LSSPKAVRDFLRLQLQNRPVEVFVGLFLDAQNRVIEVEELFSGTLTQTSVFPREVVRR
                     ALHHNAAGVIFAHNHPSGVAEPSHADETLTQALKQALALVDVRVLDHFVIGRGAALSF
                     AERGLL"
     gene            6790..7026
                     /gene="rpmB"
                     /locus_tag="VE030_08835"
     CDS             6790..7026
                     /gene="rpmB"
                     /locus_tag="VE030_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HZF23556.1"
                     /translation="MARVCEVTGKKPMGGNHVSHANNKTKRRFLPNLQYRRLWVESEN
                     RWVSLRLSQAGLRTIDKKGIDLVLAEMRARGNKS"
     gene            7045..7200
                     /gene="rpmG"
                     /locus_tag="VE030_08840"
     CDS             7045..7200
                     /gene="rpmG"
                     /locus_tag="VE030_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HZF23557.1"
                     /translation="MREKIKLESSAGTGHFYTTTKNKRTTPDKIEMKKFDPVARKHVL
                     YKETKLK"
     gene            complement(7266..8468)
                     /locus_tag="VE030_08845"
     CDS             complement(7266..8468)
                     /locus_tag="VE030_08845"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HZF23558.1"
                     /translation="MNLNGFTDLPGWAGGLINLPWWGYVVVALVFTHITIASVTIYLH
                     RYSAHRALDLHPVVAHFFRLWLWLTTGMSTKAWTAIHRKHHAKCESEEDPHSPQVLTL
                     KKVMLEGGELYRKEAKNQDTLDKYGHGTPEDWVERNVYSGIDKGGVALMLIVNILLFG
                     PIGLTVWAVQMLWIPVTAAGVINGVGHYYGYRNFQVEDASTNIVPWGILIGGEELHNN
                     HHAYASSARLSNKWYEFDIGWLYIRLMEIVGLAEVKKLAPTVKIATGKVRCDLQTLQA
                     VITHRYDVLARFTRLVKATCADELRNIRERRPGHTIDLQSVKRWLHLDAKLVPAKEKA
                     ELEEVLKTSKVLDTVYTMRQELVMLWQRSTATKEQLLSQLEDWCRRAERSGIAPLREF
                     SLTLRRYA"
     gene            complement(8629..9948)
                     /locus_tag="VE030_08850"
     CDS             complement(8629..9948)
                     /locus_tag="VE030_08850"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HZF23559.1"
                     /translation="MKNQSEISRLLTGLFEDLQQGVILWQVVVIALSLLAAWHLARRL
                     RKELHRAAGTDLDRTQRISVGGMNREVFPVTALLILLAGRWILHYFQPVHLLNIAIPL
                     VLAMVIIRGVVYLLRHTFNPGGALRSWEMAVTWIVWLGVALYLTGMLPEIGRFLEDTS
                     FRVGTQRVSLAMVLTALLTVVLTLLAALWIGRMIETRIMTLQDLEINLRFAFAKIIRS
                     VLVVTAVLIALPIVGIDITVLSLFGGAVGVGIGFGLQKVASNYISGFTILLDRSVSPG
                     DLVTIEGFYGEVTKLTARCIVVRGQDGTEAIIPNEKVVTSTVINHSYSNRQVLMRIPV
                     QISYNSNLETAMKLMLEAARSHPRVLREPAPAALLTSFADDGIKLELMAWIEDPERGK
                     LNLQSDLNLALYKGFAANEIEIPFPQRDVRIVGTVQTAVQSTQEPAK"
     gene            complement(9973..10554)
                     /locus_tag="VE030_08855"
     CDS             complement(9973..10554)
                     /locus_tag="VE030_08855"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HZF23560.1"
                     /translation="MKRTRPVRLYGLLLVLLLLAQPARAFESSDIVPGTGTIEYAFTP
                     GDDAAGLIVRAIDGARFQILVQAFSFTHAEIAGALIRGERRGLDVKVIADAEQIDLID
                     HNVIPQLVGAGIPVFTDAEHAAAHNKVIVIDAGAKTPVLITGSFNFTHAAQFRNAENL
                     LVLRGNHELTRAYLDNWMRHLKHSTRFRRGNGK"
     gene            complement(10551..11798)
                     /locus_tag="VE030_08860"
     CDS             complement(10551..11798)
                     /locus_tag="VE030_08860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HZF23561.1"
                     /translation="MIKAAAAAVDLVLPLTHPADVVLRIFFNANRELGSHGRAIVAET
                     VFTVLRHKRLLERLIAHPTPRKLVLGSLLKLQGYSLGQLEPLLKAEDRDWAVQLKSAD
                     VESLPPAVRLSLPNWLYERLVAEYAEQEAVAFGRASLKSAPLDLRVNTLLADRDQVLE
                     ELRVSGFAAEPTRWSPIGIRLAGKPAINRHPLFTRGAVEVQDEGSQLLGYLVAPKRRE
                     MVVDFCAGAGGKTLMMGALMQSAGRLYALDVSEKRLNNLKPRLKRSGLSNVHPQLIQG
                     EKDIKVKRLAGKIDRVLVDAPCSGLGTLRRNPDLKWRQSPEAVVELSAKQRAILNGAA
                     VLLKPGGRLVYATCSVLPEENQAIIEQFLGIHADFQLVSCQEVLDQQRIALESGVFLE
                     LRPQRHDTDAFFAAVLERRPRTK"
     gene            complement(11817..12509)
                     /locus_tag="VE030_08865"
     CDS             complement(11817..12509)
                     /locus_tag="VE030_08865"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HZF23562.1"
                     /translation="MRHVTLLILFLLAGPLAAAQPPQRVQIDFAVTSGSMQLGEGRDF
                     LEHDGKKYSVVSETKTVGVAALFYKLNIHRESRGLLTASGLRPLRFEENNSRKPRRAA
                     EFDWDTGQVKLTDGDNIDTVPLSANTFDPTSLPYAFAFAQSNQETMKVFIADGRRVVD
                     YEYRIIGKETLKTPLGELETLHFQKVRDADDKRGLEFWLSVGRHFLPVKIRFVEKNGT
                     IVDSTVTSITFQ"
     gene            complement(12515..13141)
                     /gene="purN"
                     /locus_tag="VE030_08870"
     CDS             complement(12515..13141)
                     /gene="purN"
                     /locus_tag="VE030_08870"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HZF23563.1"
                     /translation="MKRIVILISGRGSNLQSILEAKLDARIAAVISNNPAASGIDIAR
                     NFGVDTAVIDHRTFPDRASFDAALAEKADSYRPDLLVLAGFMRILTEAFIKRFGGRIM
                     NIHPSLLPALPGVDTHRRALQEGVRIHGCTVHFVTPELDHGPIIVQAAVPVLADDDEG
                     RLAARVLEQEHRIYPQAIRWFLDGKLKLEGNRVTVSAGRRAPDAMVCP"
BASE COUNT         2760 a         3968 c         4188 g         2663 t
ORIGIN      
        1 tgctctgaga acggttccat gtccgcgatg ttgagaatgc ggccgtcgcg atcgatgaac
       61 gcgaccgaca ggggaatccg ggtgttcttc atccacatcg cgttgacgcc ggcttcaagg
      121 taactgaaca ccatcccgct gctgtcgccg agactgtcgc gaaacatcag tccgcggcgc
      181 cggctctctt cagtgttggc aacttcggcg cggatttcgt gattcctgat tcgcagcgga
      241 taggtcagga gcagatcagc cggcgtggtc ccggtgaaac cgacgaacac ggtgatcagt
      301 actgcgggat acagcgagca tttcacggaa ttcatcgcga tttcgacagg tgaaacaaaa
      361 attggatgcg cagggatatc gagtttgacc gttcatccgg tatttcgtcc aggtcagcga
      421 gccgcagcgg caaggcgatg ccgcgcctgc acagagcgcg tttgcgtgtg gatgccggcc
      481 tgccagggag ggcgccgcgg tccgtggacg cgcgcagcgg caagcgcctg cttgccgcag
      541 gacatcgctt gctcacggcg tcgcatcgca ggaaggcgca atgcacggtc gatgtttgtg
      601 cggcgcaata aaccggaaac ccagtcccgt cgcggcttcc gggcagaaat gtgttgacaa
      661 cacccagggc ttgctcttat ttttgatctg ctgtgaagct tgtctgattc ctcagtttcc
      721 aaaggttggt tcagccttgc tttgcgcaat caagttgtaa atcaaaacca atgcgtggcg
      781 ccgcacttca cttcagcgtg ctacgcactt cttcgggagg ttttcaataa aatggtgaag
      841 caatcgttgc acttcaaacg tgctgcggtg tccctcgctg caatcgcggc gttcggtgct
      901 tccggagtag tctccgcgca aggtgtggat gtgcttgagt ccaagctcta tcccaactat
      961 gtcacggacg acatgctcct gaatgccgac aaggacgcgc agaactggct ccactacggt
     1021 cgtgactatc agaccacccg ctacagcccg ctgtcccaga tcaaccggga caacgtcaag
     1081 aagctgagac ccgcctggaa cctgtcgttc ggtgttctgg aaggtcaaga cagccaggca
     1141 gtcgcggtga acggaatgat ctatgtcacc acttccttca acaaggtcat tgcggtcgat
     1201 gccgtcagcg gcaaaaccca gtggaaatac gagcgcgaac tgcccggcga cgtgttcccg
     1261 aaactgtgct gcgacgtggt gaaccgcggt gtggccgtct acaagaacaa ggtctatctg
     1321 gcaactctcg acgctcacat cgttgcgctc gacaatacca ccggcaaagt ggtctgggac
     1381 aagaagatgg gtgactacac ctacgccgag actttcacca tcatgccgat ggcgctgcgc
     1441 ggcaaaatca tcttcggtac tgccggtgct gagtacggtg tgcgcggctg gcttgcggca
     1501 atcgacgccg acaccggcgc tcccgtctgg aagacctaca ccatccccgg acccggcgag
     1561 cctggtaacg atacctgggc tggcgaatcc tggaagtatg gtggcggttc cacctggatt
     1621 accggtgcct acgacaagga aaccaacacg ctgtactggc cggttggcaa ccctggtcct
     1681 gacttcgacc gccacgtgcg tctcggtgac aacctgttca ccaacagcaa cctggtgctc
     1741 gatccggaca ccggcaaaat caggagttat ttcaactaca cgcccaatga tccgtacgat
     1801 tacgacggcg tgaacgagat gatcctggtc gacgtcggcg gcaagaaact gtggctccat
     1861 ggctcgcgca acggtcacct gtacggtatc gatcgcgtca acacggcaaa gggcaaggcc
     1921 ggcaacgagc acaagtgcgt gtgggtcagc gcattgcagc gagtgaactg gaccaagccg
     1981 atcaccccgg ccaacaactg caagccgatc tacaactatc ctgagaagga tgtggtgtac
     2041 gacaaggtga ctaccgatat tgctccgtct cttgacggcg gcaaggaatg gcacccgatg
     2101 gcttacagcc accgcaccaa gatggtttac gtgccggtgt acgacttcgc gatggatctc
     2161 caggccaaga agatggaatg gaagcgcggc gagtggtacc tgggcgcgaa ggtcatcacc
     2221 ttcaacgcgg gcgcgggcgc gatcaagggt tatgacgcgg cgaccggcaa actgggctgg
     2281 caaaagtccc agagctatcc ggcaacgagc ggcattctgg caaccgccgg cggcctggtg
     2341 ttctatggtg atccggaagg taatgtcaac gctgtgaacg acgaaactgg cgaacacctg
     2401 tggtcgttcc aggtcggttc cggggtgcac ggcaatccga cttccttcac cggaccggat
     2461 ggcaagcagt acgttgccgt cgtttacggt gcgggcggcg gcggcatctg gcctctgttc
     2521 tacggtgagt atctcaagaa gaatcagaag ggcggcggcc tgatggtgtt cactgtcgag
     2581 tgacattgag caggaaacgc cgaaccagta atttgcactg acagctgtac caaggggggg
     2641 caaagaaact gccctccctt attttttgtt cagacaacgg cgaagaccgc tgcgccccgg
     2701 catcggggta aactgacaaa gcctcgagag cgttaacgca atgaaaaggg gaacgacctg
     2761 acagattatc aaggagttca aagtgcgtaa gttgttgaca ctatcagggc tcgccctgct
     2821 gttgatcctg gctgcagtcg tggcccgtgc acaagtagcc gatcccgatg aaatttccga
     2881 cacgctcgaa cgctcaaaag ccaagggtaa gctgatcgcc tgtgccgacc cctacaattt
     2941 cccctatgcg gcgcaaaaca tcgacccgcc gggtttcgac atcgagataa ttcgcgatat
     3001 tgccaagcgc ggcggcatgt cgctggagat gtactgggct gatacgggta cgcgcggtgg
     3061 cacgtcgagg gcattccgca attcgatcct gaagaagcgc tgtgacgtgt ttctcggcct
     3121 ttccgacagc ggcgacgatg acatgctcat ggggcaactg gccttcacca aaccctacct
     3181 gggtctgggc tacgtgctcg tggtccaggg caaggcagag ggcatgaaga gcattgccga
     3241 actgaagaag gccaacatca aggtaggcgt tcccatgtcc acgccgatcg acgactacct
     3301 gttcaccaac ggggtaccca gagatttgtt cctggacaac cgccgtatca tggaaagcat
     3361 ggccaagggc gagctcgacg cgagcctggt gtgggccact gcgatcgcgg tggcgcgtcg
     3421 cgaatatccg aaggtcacgt ttcgcatggt cgacggttac gtgccggaag ccgaacagcg
     3481 ctggaacctg aatttcgtag tgcgcaagca ggacaaggcg ctcatggagt ttatcaacgg
     3541 cggcattgca gaactgcttt ccaacggcag gatgaagcag atcgtcgaga gctacggcgt
     3601 gccgttttac ccgccgtttt cataagcaat tttcagggag gtttgttcga tatggttggt
     3661 tcgaaactca aggcgtttct cgggcagtgg gccgcgttgg ccgttgtggc gctcgccctt
     3721 ggcctgggga ccgcagttca acccgcggct gcacagcaca aggccaatga aaacgatatg
     3781 gaagtgccga cgaacaacat ggagatcgta cagcagggca gggccaagta cgcgcagcgc
     3841 tgctccttct gtcatggcgg cgatggccac ggcggcaagg gcccgtgcct gacctgcggt
     3901 gtcttcaagt ataccggtaa caccaactcg gagatttacg tcacgatcgc ggtcggtgtt
     3961 cccaggtcgc tgggaggcac gatgggagcc ttcggaacga ccatgtccca ggaagaaatt
     4021 ctcagcgtga ttacattcct gcgctgggag gagaaacgcc gcattgcggc gggggaggtt
     4081 cccgacccga agttgaacaa gggcaacgaa ctgccggtgt tcccgaccac gaactgaccg
     4141 ggaacagagc ggcacgcggc tgccgttcgc gagaaacgga aaagccggcg cgagccggct
     4201 tttccgttgc ggtcgtcgcg aggccggtca atgcttgccg gtactgccga agcctccggc
     4261 cccgcgcacg cttgctgcga actgttctac tactttgaat cccacctgta ccaccggtac
     4321 cacaaccagt tgggcgatgc gctccatcgg gttgagggta aaggcattcg atccacgatt
     4381 ccataccgag acgaagatct ggccctgata gtctgaatca attaagccga ccagattgcc
     4441 gagcacaatg ccgtgtttgt ggccgaggcc cgagcgcggc aggaccaccg cggcgagtcc
     4501 cgggtcatcc agatggatcg ccaggcccga ggggatcaac tcggtctgcc ccggcgtgaa
     4561 cgcgatcggt gcgtcgatgc aggcgcgcag gtcgagcccg gcagatccgg cggttgcgta
     4621 agcgggaagc tgatcgcgca ggcgcacgtc gagaatcctg acgtcgatgg tgtgcatgga
     4681 accttgttgt tgttgggacg ggacgcctaa gccgtaacgg gcgttttgcg tgggcggtaa
     4741 agtttggcga tatggcggat cagggcgcgt gccagctcga ttttcgatgc acgcaccatc
     4801 cggtgaaccc catcgtcgtc gaacaacacc agttcgcttt cgtcggcgcc gatcgcggcc
     4861 tgggcaaggt tcgccgcgag caacggcagc ttcttgcggc ggcgcttggc ttccgcgttc
     4921 tgctccaggt tttcgctctc ggccgcgaag ccgacgcaga acggagggtt gggacgccgg
     4981 gcaacctctt cgatgatgtc cggattgggc acaagttcca gcgtcgggat tgcgccgtct
     5041 ttcttgatct tctgcggttt gttattgctg acatgatagt cggccaccgc ggcgacgccg
     5101 acgaagatgt cacatctaac gacatgcgcg tttaccgcgt cgagcatctg tcgtgccgtc
     5161 tcgacgcgga ccagatgcgt ggatgccggt gcctcaagcg atactgggcc cgagatcagc
     5221 gttacctgtg cgcctgcctc cagcgcggcg cgcgcaacgg catagcccat tttccccgaa
     5281 ctgcggttgg tgatgcctcg taccgggtcg attggttcga aagtcggtcc ggcggtaatc
     5341 agcacgtgcg tgccggcaag cagcttcggt tggaaccatg cgccgatcga gcgggccaga
     5401 tccgccggtt cgagcatgcg ccccatcccc gtttccccgc aggcctggtc gccggaatcg
     5461 gggccgagga cgacgagccc gtcccgatgc aactgctcga tgttgcgctg ggtggcgggg
     5521 ttttcccaca tctgtcggtt catcgccggc gctaccatca gcgggcactc gcgcgccagg
     5581 caaagcgtgg acaataaatc gtctgcggcg ccgtgtacca gcttggcgat gaagccggca
     5641 gtcgcgggag cgaccacgat gatgtctcga tcgcgcgaca actcgatatg gggcatgccg
     5701 tttggcgtcc ggctgtccca catgtcggtg aacaccggct tgcccgacag cgcctgcatg
     5761 gtcaccgggg taacgaagcg gcacgccgat tcggtcatga tgacttgcac gtctgcgccc
     5821 tggcgctgca gttcgcgcag cagttccgcc gacttgtagg cggcaatgcc accagtgacc
     5881 cccaggagga tccgcttatt gctgagttcg gtcattacca tagcctaccg gactttccgg
     5941 atcggcagtg taaactgttg ttggcaaata caaaagtgcg gagggagcgg taaatggcga
     6001 tcaccgactg gccggcagac gacagaccgc gggagaaatt gctggccaag gggccgggcg
     6061 gcctttccga cgccgaactg ctcgcgatct tcctgcgcac gggggtcaag ggcaagagcg
     6121 cggtcgacct tgcgcgcgag ctgctcgttc gatttggctc gctgggagaa ttggtcgcag
     6181 ccagtcacga gagcttctgt tccgtacccg gtcttggaga cgccaagtac gtgcagttgc
     6241 aggccgcgat ggaaatggcg cgacgcgcgt tggtcgagga catgaagggc ggaatcaacc
     6301 tcagttcgcc caaggcagtg cgcgattttc tgcggctcca acttcagaat cgcccagtgg
     6361 aagtcttcgt gggcctgttt ctcgacgccc agaaccgggt catagaggtc gaggaattgt
     6421 tctccggaac actgacccag accagtgttt ttcccaggga agtggtgagg cgggcgctgc
     6481 atcacaatgc ggccggtgta atcttcgcgc acaaccaccc gtccggcgtg gcggagccca
     6541 gccatgctga cgaaaccctc acccaggcgc tgaagcaggc actggctttg gtggatgtcc
     6601 gcgtgctcga ccatttcgtc atcgggcgcg gcgcggccct gtccttcgcc gaacgcgggt
     6661 tgctctagac gaccgggcca tctgtggagg ttgagcaggt tgccggaagt gtgctataaa
     6721 gcgcgctctt tagaatttag tggggctgcg ctcggcagct tcatccggaa caaagctgga
     6781 gaagccatca tggcgagagt ttgtgaagtc accggcaaga agccgatggg cgggaaccat
     6841 gtgtcccacg ccaacaacaa gaccaagcgc cgtttcctgc ccaacttgca gtaccgccgc
     6901 ctttgggtcg agagcgagaa ccgctgggtg agcctgcgct tgagccaggc cggcctgcgc
     6961 accatcgata agaagggtat cgacttggtg ctggcggaaa tgcgcgcccg cggcaacaag
     7021 agctgatgca ggaggtaaga ggatatgcga gagaagatca aacttgaatc gtccgcgggc
     7081 accggacact tctataccac caccaagaac aagcgcacca ctccggacaa gatcgaaatg
     7141 aaaaaattcg atccggtggc acgcaaacac gttctctaca aggaaactaa gctcaagtaa
     7201 cccgtcctta gtcgcaggaa taaaaaaagc ccgcttgcgc gggctttttt ttcgtctgtc
     7261 gatccttacg cgtaacggcg cagcgtcagc gagaactcgc gtagcggcgc aatgccgctt
     7321 ctttcggcgc gacggcacca gtcctcgagc tgtgacagca actgttcctt ggttgcggta
     7381 gagcgttgcc acagcatgac cagctcctgg cgcatggtgt aaacggtgtc gagaaccttg
     7441 ctggtcttca gcacttcttc cagttcggcc ttctccttgg ccggaaccag cttggcgtcg
     7501 aggtgcagcc agcgcttgac gctctgcaaa tcaatggtat ggccgggcct gcgctcgcga
     7561 atattgcgca gttcgtcggc gcaggtagcc ttcaccaggc gggtgaagcg cgccaacacg
     7621 tcgtaacgat gggtgatcac tgcctgcagg gtctgcaggt cgcaccttac cttgccggtc
     7681 gcaattttga cggtaggggc gagttttttg acctcagcca gtccgacgat ctccatgagg
     7741 cgaatgtaca gccagccgat gtcgaactca taccacttgt tggacagtcg cgcggacgag
     7801 gcataggcat ggtgattgtt atgcaattcc tcgccaccga tcaggatgcc ccaaggcaca
     7861 atgttggtgc tggcgtcttc tacctggaag ttgcggtagc cgtagtaatg ccccacaccg
     7921 ttaattacgc ctgcggcggt gaccggtatc cacagcatct gcactgccca gaccgtcagc
     7981 ccgatcggtc cgaacagcag gatgttgacg atcaacatca gcgccacgcc gcctttatcg
     8041 atgccggaat agacgttacg ctcgacccag tcttccgggg taccgtgtcc gtacttgtcc
     8101 aaagtatcct ggtttttcgc ttccttccga tacagctcgc cgccctcaag cataaccttt
     8161 ttgagagtaa gcacttgcgg actatgcggg tcttcctcgg attcgcactt ggcgtggtgc
     8221 ttccggtgaa ttgcggtcca cgccttggtg gacataccgg tagtcagcca caaccatagg
     8281 cggaaaaaat gcgcaacaac agggtggagg tcgagtgcac gatgcgccga ataccggtgc
     8341 agatagatgg tcacggacgc gatggtgatg tgggtgaaaa cgagcgccac gacaacgtaa
     8401 ccccaccagg gtaggttgat cagcccgcca gcccagccag gcaggtccgt aaacccgttc
     8461 agattcatgc agtcaacccc gaataatcat gaagataagt atcattttac agtaaccttt
     8521 gcgacctgca agcttcaatt tgtaacgccc ggttggaaac agccaaaagc caacgtacat
     8581 cagatcgcta aagccgccta atttctccga tctacgacga aaatgcggct atttagccgg
     8641 ttcttgagta gattggacag cggtttgaac cgtccccacg atccggacgt cacgctgcgg
     8701 gaagggaatc tcgatctcgt tggcggcaaa gcccttgtag agcgccaagt tgaggtccga
     8761 ttgaaggttc aattttccgc gctccgggtc ctcgatccac gccatgagtt ccagcttgat
     8821 gccatcgtct gcgaatgagg tcagcagagc cgctggcgcc ggctccctca gcacccgcgg
     8881 atgggagcgg gcagcttcca gcatcagctt catcgccgtt tcgaggttgc tgttgtagct
     8941 gatctggacc gggatgcgca tcagcacctg gcggttggag taggaatgat tgatcaccgt
     9001 cgaagtgacc accttctcgt tggggatgat cgcttcggtg ccgtcctgcc cgcgcaccac
     9061 gatgcagcgc gcggtgagtt tggtcacctc gccgtagaaa ccctcgatcg tgaccaggtc
     9121 gccggggctc accgaacggt caagcagtat ggtgaaaccg ctgatgtagt tggatgcgac
     9181 tttctgcaga ccgaagccga tccccacgcc caccgccccg ccgaacagcg acaacactgt
     9241 gatgtcgata ccgacgatcg gcagagcaat gagcaccgcg gtgaccacca ggacactgcg
     9301 gatgatcttg gcgaacgcga atcgaagatt gatctcgagg tcttgcaagg tcatgatccg
     9361 cgtctcgatc atgcgcccga tccagagcgc ggccaggagc gtaagcacga ccgtaagcag
     9421 cgcggtgagc accatcgcaa gcgagacacg ctgggtgccg acgcggaagg acgtgtcctc
     9481 gaggaacctg ccaatctccg gaagcatccc ggtcaggtaa agtgctaccc ccagccagac
     9541 gatccatgtg accgccattt cccaggaccg cagtgccccg ccgggattga acgtgtgcct
     9601 gagcaggtag accacgccgc gaatgatcac catcgccagt accaggggaa tggcgatgtt
     9661 gaggaggtgc accggctgga aataatggag tatccagcgg ccggccagaa ggatcagcag
     9721 cgcagtcacc gggaagacct cgcggttcat cccgccgacg ctgattctct gggtgcggtc
     9781 gaggtcggta ccggctgcgc gatgcagttc tttgcgcaac ctgcgggcga gatgccacgc
     9841 tgcaagcagc gacagcgcga tgacgacgac ctgccagagg atcactccct gctgcagatc
     9901 ctcgaaaaga ccggtcagaa gacgactgat ttcggactga ttcttcatca cggtccctgg
     9961 cggaattcta cgtcacttcc cgttgccgcg gcgaaaccgg gtggagtgct tgaggtggcg
    10021 catccagttg tccaggtagg cgcgcgtgag ttcatgattt ccgcgtaaca ccagcagatt
    10081 ctcggcgtta cggaactggg cggcatgggt gaaattaaag ctgccggtga tcaggaccgg
    10141 tgtttttgcg ccggcgtcga tgactatgac tttattgtgg gcggccgcgt gctctgcgtc
    10201 ggtgaatacg ggtatgccgg ctccgactag ttgcggaatc acgttgtgat cgatgaggtc
    10261 gatctgctcc gcatcggcga tgaccttcac gtccaaaccg cggcgttcgc cgcgaatcag
    10321 cgcgccggca atctctgcgt gcgtgaagct gaacgcctgc accagaatct ggaaacgtgc
    10381 accatcgata gcgcgcacga tcagccccgc agcatcgtcg cccggggtaa acgcatactc
    10441 gatggtgccg gttccgggaa caatgtccga tgattcaaag gcgcgggccg gctgcgccag
    10501 aagcaggagt acaagcagaa gtccgtaaag ccggactggg cgcgtgcgct tcatttcgtg
    10561 cgaggacgcc gctcaagtac cgcggcgaag aatgcgtcgg tgtcgtgccg ttgcggacgc
    10621 agttccagaa acacgccgct ttcgagagcg atccgctgct ggtccagaac ctcctggcag
    10681 ctcaccagtt ggaagtccgc gtggatgccg aggaactgct cgatgatagc ctggttctcc
    10741 tccggcagca cgctgcaggt cgcgtaaacc aggcgcccgc cgggcttgag cagtaccgcc
    10801 gcgccgttca ggatcgcccg ctgcttggcg ctgagttcca ccaccgcctc gggcgactgg
    10861 cgccatttga ggtcggggtt gcgccgcagc gtgccgagcc cgctgcaggg agcatcgacc
    10921 agcacccgat cgattttgcc ggccaagcgt ttcaccttga tgtctttctc cccctggatc
    10981 agttgcgggt gcacgttgga aagtccggaa cgcttgagcc gcggtttgag attgttgagc
    11041 cgcttctctg aaacgtcgag tgcgtacagg cgccccgccg actgcatcag cgcgcccatc
    11101 atgagggtct tgccgcccgc cccggcacaa aaatccacca ccatttcgcg ccgcttcggc
    11161 gcgaccaggt atccgagcaa ctggctgccc tcgtcctgaa cctcgaccgc tccgcgggtg
    11221 aacaaagggt ggcggttgat ggcgggtttg ccggcaagcc ggatgccgat cggagaccag
    11281 cgtgtcggct cggcagcgaa tccactgacg cggagttcct cgagtacctg gtcccgatcc
    11341 gccagcagcg tgttgacccg gaggtccagc ggggcggact tgagcgaggc gcggccgaag
    11401 gcgactgctt cctgctccgc atactcggcg acaagacgtt cgtacagcca gttggggaga
    11461 ctaagcctga ctgccggagg cagcgattcc acatcagcgg acttcagctg aacggcccag
    11521 tcgcgatctt cagccttgag caggggttcg agttgaccga ggctgtaacc ctgcagcttg
    11581 agcaacgacc ccaacaccag cttgcgcggg gtggggtgcg caatcagcct ctcgagcaag
    11641 cgtttgtggc gaagcacggt gaaaaccgtt tcggcgacga tagcgcggcc gtggctgccg
    11701 agttcgcgat tggcgttgaa aaaaatgcgc aggacgacat cggccgggtg cgtcagcggc
    11761 agcaccagat ccacggcggc agcggccgcc ttaatcaagg ctgccgtggg ttgcatctac
    11821 tggaaagtga tgctggtgac ggtggaatcc acaatggtgc cgttcttttc gacgaagcga
    11881 atcttgacag gaagaaagtg gcggcccacc gaaagccaga attcgagccc gcgcttatcg
    11941 tcggcgtccc gcactttctg gaagtgcaaa gtttccagtt cgccgagcgg ggtcttgagc
    12001 gtctccttgc cgatgatcct gtactcgtag tcgaccacac gccggccgtc ggcgatgaac
    12061 accttcatgg tttcctgatt cgactgcgcg aatgcgaagg cgtaaggcag gctggtaggg
    12121 tcgaaggtgt tggcgctcag cggcacggtg tcaatattgt cgccgtcggt gagtttcact
    12181 tgccccgtat cccagtcgaa ttcagcggca cgcctgggct tgcgactgtt gttctcctcg
    12241 aatcgcagcg gccgcagccc gctggccgtg agtaaacccc ggctttcgcg gtggatgttg
    12301 agcttgtaga aaagcgcggc taccccaacc gtcttggtct cgctgaccac ggaatacttc
    12361 ttgccgtcgt gctcgagaaa atcgcggcct tcgcccagtt gcatcgagcc ggaagtcacg
    12421 gcaaagtcga tttgaaccct ttgcggcggc tgcgcggccg ccaacggacc tgccagcagg
    12481 aagagaatca gcaaagtgac gtgtctcatc gctttcaggg gcagaccatt gcgtccggag
    12541 cgcggcggcc cgcacttacc gtgacgcggt tgccttccaa cttgagcttg ccgtcgagga
    12601 accagcggat cgcctgcggg tagatgcggt gttcctgctc gagcacgcgc gcagcgaggc
    12661 gtccctcgtc atcgtcggcc agcaccggca ccgcggcctg cacgatgatc gggccatggt
    12721 cgagttcggg cgtgacgaag tgcaccgtgc agccgtgaat tctgacgcct tcctgcaacg
    12781 cgcgccggtg cgtgtccacg cccggaaggg ccggaagcag ggatggatgg atattcatga
    12841 tccggccgcc aaagcgcttg atgaaagcct cggtaagaat gcgcatgaat cccgccagca
    12901 ccagcaggtc gggacggtac gaatcagctt tttcggcaag cgcggcatcg aaactcgcgc
    12961 gatccgggaa cgtccggtga tcgatcaccg cagtgtccac tccgaagttg cgtgcgatgt
    13021 cgatgccgct tgccgcaggg ttgttgctga tcaccgcggc aatccgtgcg tcgagttttg
    13081 cttccaggat cgactgcagg ttgctgccgc ggcccgaaat caggataacg atgcgtttca
    13141 tcctgtgttt ctagtgtcct gttccgctga ttcgcggaat aactttcgaa ccgtggaagt
    13201 cggtcgcgcg acgtatttga cagcaatcca tcatcgatcg gaaaacctgc gaaatcggtg
    13261 tccggcaagg cgcgagccct tgacagtggc gggggccggt ggaacgtgga gcaggggccc
    13321 cgcttgcggg gatgcgaccg tgcaaccggc caatagcgtg tcgatcaccg gcctgacgcg
    13381 tgtctccgcg gtcagatccc cagaggggcc cctcttccgc gctccgacac gcacggcgag
    13441 gactcgtaac acagcatggc gccgattctc gtcgggtttt cagccgcgaa ttggcggaac
    13501 aggacactaa gtcatcatgc ctgcccgcct ttcgatgccg gccgtgagag ccggcgtcgc
    13561 actgtgcctg ctgctcgcg
//
LOCUS       DASQOP010000217         4310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1909806, whole genome shotgun sequence.
ACCESSION   DASQOP010000217 DASQOP010000000
VERSION     DASQOP010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1909806"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VE030_08875"
     CDS             <1..266
                     /locus_tag="VE030_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombination factor protein RarA"
                     /protein_id="HZF23564.1"
                     /translation="VQSDKSRPVPEHLRNAPTKLMKELGYGRTYRYAHDEPEAYAAGE
                     SYLPDGLDGVNFYQPTSRGLEGKLAEKLAHLRELDSKAKKNKD"
     gene            514..1797
                     /gene="serS"
                     /locus_tag="VE030_08880"
     CDS             514..1797
                     /gene="serS"
                     /locus_tag="VE030_08880"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HZF23565.1"
                     /translation="MLDIQLLRNDLDSVAARLATRGVTIDVAAFRALEEQRKHAQVRT
                     QELQAKRNASSKSIGELKRKGEDTSALLAEMAALGDQLKGIEAELEAIQAKLTDLLLG
                     IPNLPHPSVPVGKSPEDNVEVRREGEPRRFDFPVKDHVDVGAGLGMLDFDAAAKISGA
                     RFTLMKGPLARLHRAIAQFMLDIHTGEHGYTEVYVPYLVNADSMRGTGQLPKFEEDLF
                     AVPRVDGSKFYLIPTAEVPVTNIVRDEILPAGKLPLKFVCHSPCFRSEAGSYGKDTRG
                     MIRQHQFDKVELVQIVQPEQSYQGLEELTEHAETILRRLELPFRTVTLCTGDMGFAAA
                     KTYDIEVWLPGQNAYREISSCSNFEAFQARRMQARFKGEKGRNEFVHTLNGSGLAVGR
                     TLVAILENYQNKDGSITIPEALCPYMGGLAILRPI"
     gene            complement(1802..3142)
                     /gene="mltF"
                     /locus_tag="VE030_08885"
     CDS             complement(1802..3142)
                     /gene="mltF"
                     /locus_tag="VE030_08885"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008112.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HZF23566.1"
                     /translation="MLIERPALPFGTTDELVVLVRNGPTTRFLGADGKFSGMEQDMLE
                     IFAKDLGVRLRVIERSNFADILPALRRNIAHLAAAGLSAIDERRSGLIFGPAYLSVQK
                     VVAYNTDEPRPRGVRDLVGKRGAVVAGSSSADQLKLERASIPDLRWDEVPVADSMTLL
                     DQLSDGKYDFVMTDSNIVELAQNLSPNIGKAFILGGPETLAWALPSDTDPLLVNQVHD
                     FFKRINANGSLRILLDRYYGHIERLNQADVFAFLERRTSLLPRYRDVFHEAQELTGID
                     WRLLAALGFQESHWDPFATSPTGVRGLMMLTSGTADRMGVSNRLDARQNIIAGARYLK
                     ILKDALPGRIDEPDRTWLALASYNVGFGHLEDARILAQRRGLNPDSWFDLRKTLPQLA
                     RSDFYTTVKRGFARGGEAVILTENIRNYFDILVRYEDPYRPLFSSLTPEARAEQ"
     gene            3328..3421
                     /locus_tag="VE030_08890"
     tRNA            3328..3421
                     /locus_tag="VE030_08890"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3363..3365,aa:Ser,seq:cga)
     gene            complement(3776..3928)
                     /locus_tag="VE030_08895"
     CDS             complement(3776..3928)
                     /locus_tag="VE030_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23567.1"
                     /translation="MKRSRTESVASMLLRKALFVYEYPGHLVLADSAKLKKTIKRAYY
                     AAMRGA"
BASE COUNT          902 a         1264 c         1365 g          779 t
ORIGIN      
        1 acgtccagtc cgacaagtcc cgtccggtgc cggagcacct gcgcaatgcg cccaccaagc
       61 tcatgaagga actcggctac ggccggactt accgctacgc gcacgacgag ccggaagctt
      121 acgcggcagg cgagagctac ctgccggacg gcctagacgg ggtgaatttc taccagccaa
      181 cgtcgcgggg tctggaaggc aaactcgccg aaaagctcgc gcacctgcgc gagctggatt
      241 cgaaggcaaa gaagaataag gactgaaaag ccttcaccgc agaggcgcaa aggcgcagag
      301 gaaacgcaaa ggaaagaaaa gataagataa gtaatagtga tgaggcaggg taagtgaata
      361 gtcgcgacga atgcgattca tcgattgggc ggcgatggtc ctgaattcct cccaactctt
      421 ataccggatt cgtttggttt tctctgcgtt ccctccgcgc cttcgcgcct ctgcggtgaa
      481 gatgtagtgc tgaaagcgaa atttggggtt tagatgctcg acattcagtt gctcagaaac
      541 gatctcgatt cagtggcggc acggcttgcg acccgcggcg tcacgatcga cgttgccgca
      601 tttcgcgctc tcgaggagca gcgcaagcac gcgcaagtac gcacccagga actgcaggcc
      661 aaacgcaacg cgtcttcgaa gagcatcggc gaactcaagc gcaagggcga agacacctcc
      721 gcactgttgg cggagatggc cgcgctcggc gaccagctga aaggcatcga ggcggaactg
      781 gaggcgatcc aggcgaaact caccgacctg ctgctcggca ttcccaacct tccgcaccca
      841 agcgtgccgg tgggaaagtc cccggaggac aacgtggaag tgcggcggga gggcgagccg
      901 cgccgcttcg attttccggt gaaggaccac gtggacgtcg gcgccggtct gggcatgctg
      961 gatttcgatg ccgcggcgaa aatcagcggc gcgcgcttca cgctgatgaa ggggccgctg
     1021 gcgcggctgc atcgcgccat tgcccagttc atgctcgaca ttcataccgg cgagcacggc
     1081 tacacggaag tgtatgtgcc ttacctggtg aacgccgaca gcatgcgcgg caccgggcag
     1141 ttgccgaagt tcgaggagga cttgttcgcg gtgccgcggg tcgacgggag caagttctac
     1201 ctgattccga ccgcggaagt gccggtgacc aacatcgtgc gcgacgagat cctgccggcc
     1261 gggaagctac cgctgaaatt cgtctgccac tcgccctgtt tccgttccga ggccggctcc
     1321 tacggcaaag acacccgcgg catgatccgc cagcaccagt tcgacaaggt cgaactggtg
     1381 cagatcgtcc aacccgagca gtcctaccag ggactcgaag aactgaccga gcatgcggaa
     1441 accatcctca ggcgcctgga gttgccgttt cgcaccgtaa cgctttgcac cggcgacatg
     1501 ggattcgcgg ccgccaaaac gtatgacatc gaggtctggc tgccgggaca gaacgcgtac
     1561 cgggagatct catcctgcag caatttcgag gcattccagg cgcgacgcat gcaggcgcgc
     1621 ttcaagggcg agaagggcag gaacgagttc gtccacaccc tgaacggatc gggacttgcg
     1681 gtggggcgaa cgctggtcgc gatactggag aactatcaga acaaggatgg cagcatcacc
     1741 attcccgagg cgctgtgtcc ctatatgggc ggcctggcca tcctccggcc catttgaggg
     1801 atcactgctc ggcccgcgct tccggcgtga gcgacgagaa cagcggccgg tagggatcct
     1861 cgtaacgcac cagaatgtcg aagtagttgc ggatgttctc ggtaagaatc accgcttcgc
     1921 cgccgcgggc gaaaccgcgc ttgaccgtgg tgtagaagtc cgagcgtgcc agctgcggca
     1981 gcgtcttgcg aaggtcgaac caggagtccg ggttcaagcc gcgccgctgg gccagaatgc
     2041 gggcgtcttc caggtgtccg aagcccacgt tgtatgaggc cagggccagc caggtgcggt
     2101 ccggttcgtc gatgcgcccg ggaagcgcgt ccttgagtat cttcagatag cgcgcgccgg
     2161 cgatgatgtt ctggcgggcg tcgagccggt tgcttacccc cattcggtcg gcggttcccg
     2221 aggtaagcat catcaagccg cgcacgcccg tcggactggt ggcgaacggg tcccagtgcg
     2281 attcctggaa gcccagcgcg gcaagcagcc gccagtcgat tccggtgagc tcctgtgcct
     2341 cgtggaagac gtcgcggtat cgcggcaaca aggaggtacg gcgttcgagg aacgcgaaga
     2401 catcggcctg attcaggcgc tcgatgtgtc cgtagtaccg gtccagcagg atgcgcaacg
     2461 agccgttggc gttgatgcgt ttgaagaaat cgtgcacctg gttgaccagc agcgggtcgg
     2521 tgtcactggg cagcgcccag gccagcgtct cgggcccacc caggatgaag gctttaccga
     2581 tgttgggtga taaattctgc gccagttcga cgatgttgga atcggtcatc acgaaatcgt
     2641 atttgccgtc cgacaactgg tcgagcaggg tcatgctgtc ggccacgggc acttcatccc
     2701 aacgcaaatc cgggatactg gcgcgctcga gcttgagttg atccgcactg ctggacccgg
     2761 cgaccacagc gccgcgcttt cccacgaggt cgcgcactcc gcgcggtcgc ggctcatcgg
     2821 tgttgtaggc gaccaccttc tgcaccgaca gataggccgg tccgaagatg agcccgctgc
     2881 ggcgttcgtc gattgcgctc aggccggcgg cggcaagatg cgcgatattg cggcgaagtg
     2941 ccggaagaat gtccgcgaag ttcgatcttt cgatgacgcg cagacgcacg ccgagatcct
     3001 ttgcgaatat ctcgagcatg tcctgctcca tgccgctgaa cttgccgtcg gcgccgagga
     3061 agcgcgtggt cggaccgttg cgtacgagta ccaccagttc atcggttgtg ccgaatggaa
     3121 gcgccggtcg ttcgatgagc atgcctgggt cgcaaaaagg caggacgagc agcagcgccg
     3181 cggccgcgag cttgtgcgcg gacgacggtt ggcggcgcga gaccgccctg attgttcttc
     3241 gcctccgcgg ccggtaaaag gcgcggcgcg tgttcgcaaa gacctgcatc gctgttaaaa
     3301 tatcacgctc ctgcatgatt cgagttcgga gaggtaccga agtggccata acggcgctga
     3361 ctcgaaatca gatggacgga caaaatccgt cacgtgggtt cgaatcccac cctctccgcc
     3421 aattgaaaat gccgcctcgt gcggcatttt caattggcca cgaggatgtg gatgcgaacc
     3481 cactggttcg accacgaagc actgcttcgt gggacgcccg tagggcggtc cctcggcatg
     3541 catccgaggg atgactggat cgccataagg cgatccagtc acaatcccac cctcaagaat
     3601 ttggcgcgtc gaacgattgc caacgtgtgc ggcattttca attggccacg aggatgtaga
     3661 tgcgaaccca ctggttcgac cacgaagcac tgcttcgtgg gacgcccgta gggcggtccc
     3721 taggcatgta gccgagggat agccggatcg ccgtcgattc gcgtgtggcg ctcgatcacg
     3781 caccgcgcat cgcggcgtag taggctcgtt taatcgtctt cttcagcttc gcgctgtcgg
     3841 ccagcacgag gtgccccgga tactcataca cgaacagggc ctttctcaga agcatcgaag
     3901 caacagattc tgtgcgtgaa cgtttcattg gatactccca aaaaaatgtg ccagcaaatg
     3961 acggggcgcc tggggataat ccggagacac atgcctatag gcggcagtgg cttccgagtc
     4021 gtccggattt ccccggcgca ccctttattg cgcaatggcc atgccatcgg ggagtgtcga
     4081 aaaagtggca cttggaccgc ggttggaaag cactctgctt ccaagcgcaa aggagaggtg
     4141 tcggaaaacc gacggatgag tatgccgaac gctcagcaca aagcgcggcc gcaaagcgtc
     4201 acagaatgcc gaatgtcatt ggggcgcaga ctttctgtgc ctaaaaaaag gcgcccgata
     4261 agggaggtaa ccgggcgccc atttgcaaaa gcgcggctac ttaccgtcac
//
LOCUS       DASQOP010000218         9352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1309636, whole genome shotgun sequence.
ACCESSION   DASQOP010000218 DASQOP010000000
VERSION     DASQOP010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9352
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309636"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VE030_08900"
     CDS             <1..207
                     /locus_tag="VE030_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HZF23568.1"
                     /translation="LKRVEKEMLDAARNLEFETAARLRDQLKELKELAFGVAESDEAP
                     LAATGTDGKPAKVASIAAARKRSR"
     gene            211..705
                     /locus_tag="VE030_08905"
     CDS             211..705
                     /locus_tag="VE030_08905"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011657380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HZF23569.1"
                     /translation="MARIATPARVLFVCMGNICRSPTAEGVFRHAVASAGLKQKILAD
                     SAGTHGYHLGHPPDRRAQLAAARRGYDLANLRARQVTAQDFVEFDYILAMDLDNLSEL
                     KRLAPAQHHGEVCLFMEYSSARRGGEVPDPYYGGAQDFELVLDMAEDAAAGLLRHIKK
                     KLGV"
     gene            complement(756..3269)
                     /locus_tag="VE030_08910"
     CDS             complement(756..3269)
                     /locus_tag="VE030_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HZF23570.1"
                     /translation="MKRMLFNATHAEELRVAIVDGQKLIDLDIETAGKEQRKSNIYKG
                     VITRLEPSLEAAFVDYGSERHGFLPFKEVSRSFFQNPDADPARVSIKDALREGQELIV
                     QIDKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRVEGEDRNELRDTISQLDV
                     PPGMSIIARTAGIGRSVEELTWDLGYLMQLWRAIETAAKQQGGAFLIYQEGSLVIRAI
                     RDYFHQEIGEILIDTESIYDQARQFMGHVMPQNVNRVKMYKDDVPLFSRFQIEHQIET
                     AYSRQVPLPSGGAVVIDHTEALVSVDVNSARATRGADIEETALRTNLEAADEIARQLR
                     LRDLGGLVVIDFIDMESARNQRDVENRLRDALKYDRARVQTGKISRFGLLELSRQRLQ
                     TSLGETSHIACPRCHGTGHIRSIESSALHILRILQEEAMKENTAALHAQVPVDVATYL
                     LNEKRNEFHSIETRLKVNVLLVPNVNLQTPNYSVTRLRHDELNQTDVVQPSYRLVEVA
                     AEVAEPAATVPKPEPQRPQAAVQGITPAQPAPMRKEEPKPSILDKIFGWLTRKPEVKL
                     EPPKIRPRDPQQRRDHTRDGRGRGRGRLDEKRDERPPRQDGESRREHIRPRGQERAER
                     KPQERQQQHQQRPPRPEQGLAAVEQRQPGERPEGPREGEGRGRRRRGRRDRQGERQDN
                     VSQQPRADHPSNSPATAEMARPAREREHPAPTTPSPELQTAVLEPVMFTHEPVAAEPP
                     STIAPATLPQEPYLPETNTGEQQPEPEATAPDRQPWKMEPVALPPDMMMIETQADKAA
                     RMLEGERQDVPRPARAPRQRKPEPVIPDEPLQQVETRKE"
     gene            3621..4574
                     /locus_tag="VE030_08915"
     CDS             3621..4574
                     /locus_tag="VE030_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HZF23571.1"
                     /translation="MNTLSKGSVTWRQVDETAQDQRIDNFLHKILKGVPKSHIYRIVR
                     GGEVRVNGRRVEVAYRLQSGDKVRLPPVRTARPASEPGTRTVKAMFAGKVLLEDEDLL
                     VVDKPGRVAVHGGSGISSGVIEQLRQARSGIRFLELVHRLDRDTSGVLVLAKKRSALT
                     ALHAQLRDGAVKKHYLVLVLGEWKDAKRNVKLALHKYVSASGERRVVVDEDGAQAHTV
                     FRLERNFPGFSLLDAELKTGRTHQIRVHLAHLGFPIAGDDKYGDFDANKRLAKQGLKR
                     MFLHAASLEFRHPRSGATMRVQAPLAPELESFLRSLKTSGH"
     gene            4577..5236
                     /locus_tag="VE030_08920"
     CDS             4577..5236
                     /locus_tag="VE030_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HZF23572.1"
                     /translation="MTSGRFELLVFDWDGTLVDSAGHIVASLQAAFRDLDLPVPSPEA
                     ARHVIGLGLQDALAYLNPGLESSRYREVADRYSAHFLIGHEDIELFPLVEEGIAELNR
                     DGYLLAVATGKSRRGLDRALHSTALGAHFHASRCADEGFPKPHPDMLETLMRLLGVTR
                     ERTLMIGDTTHDLQMAANAGVPAVGLSYGAHPPEELDRLEPLACFGSFAELMQWLKQD
                     A"
     gene            5215..5580
                     /locus_tag="VE030_08925"
     CDS             5215..5580
                     /locus_tag="VE030_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HZF23573.1"
                     /translation="MAEARRLICAAGDLPERGRGVRFTVNTERGLAAAFAVRYRGRVH
                     GYVNRCAHVGVELDWQPGEFFDVSGLYLVCSTHGAAYLPDTGYCVGGPCIGQNLTKLP
                     IEERDGQVYLFENKDTLHG"
     gene            5573..6532
                     /locus_tag="VE030_08930"
     CDS             5573..6532
                     /locus_tag="VE030_08930"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HZF23574.1"
                     /translation="MAETDQGGSAGNWERDIIVKLARAGLDEQRKARHWGIFFKTLTF
                     IYLFLLMFAAFGWFGGREVAMPGNHTALVELNGVIAADTHANADNINHGLQDAFGDKS
                     TKGVILRINSPGGSPVQAATINIEIGRLREKYPDIPIYAVIEDICASGGYYVAVAADR
                     IYVDKASIVGSIGVLMDGFGFTGTMEKLGVERRLLTAGENKGFLDPFSPLDPAQREYA
                     RRMLGEIHQQFVEVVRKGRGKRLKEAPDTFSGLVWTGQKSIDLGLTDALGSVDFVARE
                     VIKAEKIVDFTPRENLAERFAKRFGASLGEALVRAAGQLSPVR"
     gene            complement(6494..7243)
                     /locus_tag="VE030_08935"
     CDS             complement(6494..7243)
                     /locus_tag="VE030_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012735014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HZF23575.1"
                     /translation="MDKGRLYLIPVDLVPGNTGDWIGTRLKTAVQALRFFVVEHPKSA
                     RQFLKALNLSLPELTLEVLDEHSGTEDVQRFVRKLEAGVDLGLLSEAGCPAIADPGAA
                     LVAEAHRIGARVVPMVGPSSIVLALMASGLNGQRFAFNGYLPARSPDRERGIRELEER
                     SRRLGQTEIFIEAPYRNDQLFAALLRSCDADTMLSLATDLTGATEQLATRTVAQWENA
                     SPNLDRRPTVFLLLAKRKVSAHRGKLPGGAH"
     gene            complement(7276..7872)
                     /locus_tag="VE030_08940"
     CDS             complement(7276..7872)
                     /locus_tag="VE030_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HZF23576.1"
                     /translation="MSSTNSGLQLILASTSRYRKDLLSRLGLAFETAAPEVDETPLDS
                     EQPTDTARRLALAKAQAVRTRFPGALIIGSDQVAVCDGVRLDKPLTHEVAVQQLRHVS
                     GRSVIFHSAVALLNASSGRIQWRLVPTTVKFRRLNDAEIERYLRREPAYDCAGSAKAE
                     GLGISLVESIESGDPTALIGLPLIALSSMLREEQLIIP"
     gene            7917..8414
                     /locus_tag="VE030_08945"
     CDS             7917..8414
                     /locus_tag="VE030_08945"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HZF23577.1"
                     /translation="MFDRAVVDSVAFARESGELRATVAVAGLARLHDALFDQSGAIAY
                     ELAGRVDQEGKPWLRLALDGELVLRCQRCLGPVRFRFEAVRSFLLVPADQALSDPADE
                     AEDTEQLYADPKLDVIALVEDEVILGLPMVAGHEEGACAAPLPKAQAGRRESPFDALA
                     DLKRQ"
     gene            8442..8621
                     /gene="rpmF"
                     /locus_tag="VE030_08950"
     CDS             8442..8621
                     /gene="rpmF"
                     /locus_tag="VE030_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HZF23578.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDFLTSPPLAVEPTTGEVHLRHHVSP
                     NGYYRGKKVLKTKGD"
     gene            8771..>9352
                     /locus_tag="VE030_08955"
     CDS             8771..>9352
                     /locus_tag="VE030_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HZF23579.1"
                     /translation="MAITIALDCMGGDHGAHVTVPAALDFVDRVDDAQVVLVGIPETI
                     ETELKRRRAKTGERIRIEAATEVVAMDEAPASALRGKRDSSMRLAIDLVKIERADACV
                     SAGNTGALMAMSRFVLKMSPGIERPAIASFLPTQRGRTCVLDLGANVDCSAEHLLQFG
                     AMGSSLVSAVERVERPTVGLLNIGEEEIKGNEVV"
BASE COUNT         1679 a         2822 c         3014 g         1837 t
ORIGIN      
        1 ttgaagcgcg tcgagaagga gatgctcgat gctgccagga acctcgaatt cgagactgcg
       61 gcgcggttgc gcgaccagtt gaaggaactc aaggagctgg cattcggcgt cgccgaaagc
      121 gacgaagcgc cgctcgcggc gaccggaacc gatggcaagc cggccaaggt cgcgtcgatt
      181 gcggccgcgc gcaagcgcag ccgatagagg atggcacgga tcgctacccc cgccagggtg
      241 ctgttcgtct gcatgggcaa tatttgccgg tcgcctacag ccgaaggcgt gtttcgccat
      301 gcggtggcgt cggcaggact gaagcagaaa attctcgccg actcggcggg cacccacggt
      361 tatcacctcg gccacccgcc cgatcgccgt gcccaattgg ccgcggcccg acgcggctac
      421 gatctcgcga atctgcgcgc ccgccaggtg accgcacaag atttcgtcga attcgattac
      481 atcctggcaa tggatctgga caatctctct gaactgaaac ggcttgcccc ggcgcaacat
      541 cacggcgagg tttgcctgtt catggaatac agcagcgcgc ggcgcggagg agaagtgccg
      601 gatccctact acggtggggc gcaggatttc gaactggtgc tggacatggc cgaggatgcg
      661 gccgcagggc tccttcgaca cataaaaaag aagctcgggg tctagccgag cttcggttgt
      721 gctgctgacg gcattggccg ccgtcgatcc gcggattatt ccttgcgcgt ttccacttgt
      781 tgcagcggct cgtctggaat gacgggctcg ggcttgcgct ggcggggcgc cctggcgggc
      841 cggggcacgt cctgtcgttc tccctcgagc atcctggccg ccttgtccgc ctgcgtctcg
      901 atcatcatca tgtcgggcgg cagcgcgacc ggctccatct tccagggttg ccggtcaggc
      961 gccgtggctt cgggttcagg ttgctgctcg ccagtgtttg tctcgggcag gtaaggttcc
     1021 tgcggcaggg tagccggagc gatggtcgac ggcggttcgg cggcaaccgg ctcgtgcgtg
     1081 aacatcaccg gttcgagaac agccgtctgg agctcaggtg aaggcgtcgt cggcgctgga
     1141 tgttcacgct cgcgggccgg cctggccatt tcggcggtcg caggagagtt cgaggggtga
     1201 tcggcgcgcg gctgctgact gacattgtcc tgacgctcac cttggcggtc gcgccggccg
     1261 cggcgcctgc ggccgcgacc ttctccttcg cgcgggcctt ccggtcgctc tcccggttgg
     1321 cgttgctcga ccgcagccag tccctgttcg ggacgcggtg gacgttgctg atgctgctgc
     1381 tggcgttcct ggggcttgcg ttcggcacgc tcctggccac gcggacggat atgttcgcgg
     1441 cggctttccc catcctggcg cggcgggcgc tcgtcacgct tttcatccag gcgaccccgg
     1501 ccgcgccccc ggccgtcgcg cgtgtggtca cggcgctgct gcggatcgcg cggccggatt
     1561 ttgggcggtt cgagcttgac ttccggcttt cgggtaagcc agccgaagat cttgtccagg
     1621 atcgaaggct tgggttcttc cttgcgcata ggggcaggct gggcaggcgt gattccctgc
     1681 actgcggcct gcggccgttg cggttccggc ttcggcacgg tcgccgcggg ctccgcgact
     1741 tcggcggcca cctctaccaa cctgtagctc ggttgcacca catcggtctg gttgagctcg
     1801 tcgtgccgca gccgagtgac ggaatagttg ggcgtctgca gattcacgtt cgggaccagc
     1861 agcacgttga ccttgagccg cgtctcgatc gagtggaatt cgttgcgctt ctcgttgagc
     1921 aggtaagtcg ccacgtccac cgggacctgc gcgtgcagcg ccgcggtgtt ctccttcatc
     1981 gcctcttcct ggaggatgcg caagatatgc agcgccgagg attcgatcga gcggatgtgg
     2041 ccggtgccat ggcagcgcgg gcaggcgatg tggctggttt cgccgagcga agtctgcagc
     2101 cgctggcgcg acagctccag cagcccgaag cgggagatct tgccggtctg cacgcgggcg
     2161 cggtcatact tcagcgcatc gcgcagccgg ttctccacgt cgcgctggtt gcgcgcggac
     2221 tccatgtcga tgaagtcgat caccaccagt ccccccagat cgcgcagcct gagctgccgg
     2281 gcgatttcgt ccgcggcttc caggttggtg cgcagcgcgg tttcttcgat gtcggcgccg
     2341 cgcgtggcgc gcgcggagtt gacgtccacc gacaccagcg cctcggtgtg gtcgatcacc
     2401 actgcgccgc cggacggcag cggcacctgc cgcgaatagg ccgtttcgat ttggtgctcg
     2461 atctggaatc gcgagaacag cggcacgtcg tccttgtaca tcttcacgcg gttgacgttc
     2521 tgcggcatca cgtggcccat gaactggcgg gcctgatcgt aaatgctctc ggtatcgatc
     2581 aggatttcac cgatctcctg gtggaaatag tcgcgaatgg cgcgaatgac caggctgcct
     2641 tcctggtaaa tcaggaacgc gccgccctgc tgcttggcgg cggtttcgat cgcccgccac
     2701 aactgcatca ggtagccaag atcccaggtg agttcctcca ccgaacggcc gatgccggcg
     2761 gtgcgggcga tgatgctcat gccgggcgga acatccagtt gcgagatagt gtcgcgcagt
     2821 tcgttgcggt cctcgccctc gacccggcgc gaaacgccgc cgccgcgggg attgttcggc
     2881 atcaggacca gatagcggcc ggccaggctg atgaacgttg tcagcgccgc gcctttgttg
     2941 ccgcgctcgt ccttgtctat ctggacgatg agttcctggc cttcgcgcag ggcgtccttg
     3001 atgctgacgc gggcggggtc ggcatcgggg ttctggaaaa aggagcggct gacttccttg
     3061 aacggaagaa agccatgacg ttcactgccg tagtcgacaa acgccgcttc gaggctgggc
     3121 tcgaggcggg tgatgacacc tttatagata ttgctcttgc gttgctcttt tccggcagtt
     3181 tcgatatcca aatcgatgag cttctggcca tcgacgatcg cgacgcgcag ttcttctgcg
     3241 tgggtcgcgt taaacaacat gcgcttcatt tttgttctcc cgcgctcaca tattcacggg
     3301 gaggacaaac ctggggggcg ccccggttgt cgaggggctc tagctttctg tcgtcgggaa
     3361 attcattggg tggaggagga cgagagattc tggcctgagg tttgtcaata cacatcctgc
     3421 ttcacgcagg agggtggaat ccacttttgg tgtgcgagcg cgtaattcat caattaccat
     3481 cgctactttt tcgggtgagc gaagttaacc cctcgttacc ggcgtgacat ggcgggagat
     3541 gaagccgctg gtttgcggcg tacgaggctc gaccgccaga ttctgaccga ttgcctgttt
     3601 gccggaatag tataagcaaa atgaatacct taagcaaagg atccgtaacc tggcggcagg
     3661 tggacgagac tgcacaagac cagcggatag acaatttcct gcacaagatc ctgaaagggg
     3721 tgccaaaaag ccacatctac cgcatcgttc gcggcggtga agtcagagtg aatggccggc
     3781 gggtggaggt tgcctaccgg cttcaatccg gggacaaggt cagacttccg ccggttcgta
     3841 ccgcccgccc agcatcggaa cccggaaccc ggacggtcaa agcgatgttc gcgggcaaag
     3901 tgctgcttga agacgaggac ctgctcgtcg tcgacaagcc ggggagggtg gcagttcatg
     3961 gcggcagcgg catttccagc ggcgtgatcg aacaattgcg tcaggcgcgg tcaggaatcc
     4021 gctttcttga gcttgtgcac cggcttgatc gcgatacctc gggcgtgctg gtgctggcca
     4081 agaaacgcag cgccctgacc gccttgcacg cgcagttgcg cgatggggcg gtgaaaaagc
     4141 attatcttgt gctggtgctg ggcgagtgga aggatgccaa acgcaacgtc aaactggcgc
     4201 tgcacaaata cgtatccgca tccggcgaac gccgggtggt ggtcgacgag gacggcgcgc
     4261 aggcgcatac cgtgttccgc ctggagcgca atttccccgg attcagcctg ctcgacgccg
     4321 agctcaagac cggccgcacc catcagattc gcgtacatct ggcgcacctc ggttttccga
     4381 tcgccggcga cgacaagtac ggtgatttcg acgctaacaa gcggctggca aagcagggcc
     4441 tgaagcgcat gttcctgcac gccgccagcc tcgaattccg ccacccccgg agcggcgcga
     4501 cgatgcgggt gcaagccccg ctcgcacccg agctggaaag cttcctgcgc tccctcaaga
     4561 ccagcgggca ctagaattga cgtccggacg cttcgaactg ctggtcttcg actgggatgg
     4621 cacgctggtg gactccgccg gtcatatcgt cgcctcgttg caggccgcgt tccgggacct
     4681 tgatcttcca gtgccgagcc cggaagcggc gcgccacgtg atcggactcg ggctgcagga
     4741 cgcgctggcc tatctcaacc ccggtctgga aagcagtcgt taccgcgaag tcgcggaccg
     4801 ctatagtgcc cactttctga ttggccacga ggacatcgag ctgtttccgc tggttgaaga
     4861 aggcatagcc gagctcaacc gcgacggtta ccttttggcg gtcgccaccg gcaagagccg
     4921 ccgcgggctg gaccgtgcct tacacagcac ggcgctcggg gcacattttc acgcttcgcg
     4981 ttgcgctgac gagggtttcc ccaaaccgca tcccgacatg ctggaaacgc tgatgcggtt
     5041 gctgggagtc acgcgagaac gcacgctgat gatcggagac accacgcacg acctgcagat
     5101 ggccgccaac gccggggtac ctgcggtcgg actgagctat ggcgcacacc cgcccgagga
     5161 gctcgatcgc ctggaaccgc tggcgtgttt cggcagtttc gccgaactga tgcaatggct
     5221 gaagcaagac gcctgatctg cgcggccggc gacctgcccg agcgcggcag gggcgtgcgc
     5281 tttaccgtga atacggagcg cgggctggcg gcggcgtttg ccgtgcgcta ccggggccgg
     5341 gtgcacggct atgtaaaccg ctgcgcccat gtcggggtcg aactcgactg gcagccaggt
     5401 gagttcttcg atgtgtccgg tctatacttg gtgtgttcga cgcacggtgc tgcgtatctt
     5461 ccggataccg gctactgcgt tggcggtccg tgcataggcc agaacctgac caaactgccg
     5521 atcgaggagc gggacggaca ggtttatctg ttcgaaaaca aggatacgct tcatggctga
     5581 aaccgatcag ggcggctccg caggcaactg ggaacgcgac atcatagtaa agcttgcacg
     5641 cgctgggctc gacgaacagc gcaaggcacg ccactggggt atctttttca agacgctcac
     5701 cttcatttat ctttttcttc tgatgttcgc cgcattcggc tggttcggcg ggcgtgaagt
     5761 ggcgatgccc ggcaaccata ccgcactggt ggaattgaac ggcgtcattg cggcggatac
     5821 gcacgccaat gccgacaaca tcaatcacgg gctccaggac gctttcgggg acaagagcac
     5881 caagggcgtg atcctgcgca tcaacagccc aggcggcagc ccggtgcagg ccgccaccat
     5941 caacatcgaa atcggccggc tgcgcgagaa atatccggac attccgatct atgcggtgat
     6001 cgaggacatc tgtgcctcgg gcggctacta cgtggcggtt gccgccgatc ggatctacgt
     6061 cgacaaggcg agcattgtcg gttcgatcgg cgtgctcatg gacggcttcg gttttaccgg
     6121 cacgatggag aaactcggcg tcgaacgcag gctgctcact gccggagaga acaagggatt
     6181 cctcgatccg ttctcgccac tcgatcccgc acaacgcgaa tacgccaggc gcatgcttgg
     6241 ggagattcac cagcagttcg tcgaagtggt ccgcaagggg cgcggcaagc gcctgaagga
     6301 agcgccggac acattttccg gcctggtgtg gacgggccag aaaagtatcg acctgggcct
     6361 gaccgatgcc cttgggagcg ttgatttcgt tgcccgtgaa gtcatcaagg ccgagaaaat
     6421 cgtcgacttc acgccgcgcg aaaatctggc cgagcgcttc gccaagcgct tcggtgcttc
     6481 gctcggcgag gcgctagtgc gcgccgccgg gcagctttcc ccggtgcgct gaaactttcc
     6541 gctttgccag gagcaggaat acggttggcc tgcggtccag gttcggcgat gcattttccc
     6601 attgcgccac ggttcgggtc gccagctgtt cggttgcgcc ggtaagatcg gttgccagcg
     6661 atagcatcgt atccgcgtcg cagcttcgca gcagcgcagc gaacaactga tcgttgcggt
     6721 agggcgcctc gatgaatatt tcggtctgcc ccaggcgtcg cgatctctcc tccagttccc
     6781 gaatgccgcg ctcgcgatca ggggagcgtg cgggcaggta gccattgaag gcaaaacgct
     6841 ggccattgag tccggaggcc atcaatgcca agacgatgga ggagggaccg accattggga
     6901 ccactcgcgc gcctatccgg tgcgcctcgg ctaccagcgc cgcgcccgga tcggcaatcg
     6961 ccggacagcc cgcttccgac agcagaccga gatcgactcc tgcttccagt ttgcgcacga
     7021 accgctgtac gtcttcggtt cctgaatgct catccagcac ttcgagggtc agttccggca
     7081 acgacagatt caatgctttc aggaactgcc gggctgactt cggatgctcc accacaaaaa
     7141 agcgcagagc ctgcaccgcg gttttcagtc gcgttccgat ccagtcgcct gtgtttccgg
     7201 ggaccagatc aactggaatc agataaagtc gccccttatc cattatttac atgaaaagaa
     7261 tacgataata cattgttatg gaataattaa ttgttcttcc ctaagcatgc tcgaaagggc
     7321 tataagcggc aggccgatga gcgcggtcgg gtcaccggac tcgatgcttt cgaccagtga
     7381 gatgcccaat ccttccgcct tggcgctgcc ggcgcagtcg tatgccggtt cgcggcgtaa
     7441 ataacgctcg atttccgcgt cgttgaggcg gcggaacttt accgtggtgg gcaccagccg
     7501 ccactgtatc cggccactgc tcgcgttgag tagtgccaca gcagaatgga aaatcacgga
     7561 cctgccgctg acatggcgca gttgctgaac ggccacctca tgggtcagcg gcttgtcgag
     7621 gcgcacgcca tcgcacaccg caacctgatc ggaaccgatg ataagcgcgc ccgggaatct
     7681 ggtgcgtacc gcttgggcct tggccagcgc gagtcggcgc gcggtgtcgg tgggttgctc
     7741 gctgtccaga ggtgtttcat ccacttccgg cgcggcggtt tcgaaggcca agccgagcct
     7801 cgaaagcaag tccttgcggt agcgcgaggt cgaggcgaga atcaactgca gaccactgtt
     7861 tgttgaagac attttgtttt gacagggtaa ttcaaaacta atattatgcg cggcttatgt
     7921 ttgaccgggc tgtcgtcgat agcgttgcgt ttgcgcgtga gtccggtgaa ctgcgcgcaa
     7981 cggtagcagt cgcggggctt gcgcgtttgc acgatgcgtt gttcgatcag tccggcgcaa
     8041 tcgcgtatga gctggcaggt cgtgtcgatc aggagggcaa accctggttg cgtcttgcgc
     8101 tcgacggcga gctcgtgctc cgctgccagc gttgcctcgg gccagttcgc tttcgattcg
     8161 aagcggtgcg cagcttcctg ctggttcccg cggaccaggc attgagcgac cccgccgatg
     8221 aggcggagga tacggaacag ctgtacgccg acccgaaact cgacgttatc gccttggtcg
     8281 aggacgaagt tattctaggt ctgccgatgg tggcgggcca cgaggagggc gcctgtgcgg
     8341 cgccgttgcc gaaagcgcaa gccggcaggc gggaatcgcc gttcgacgcg ttggcggacc
     8401 tcaagcggca gtgaaaaagt ttcaattgaa ggagtcatct catggcagtc caacagaaca
     8461 agaagtcgcc ctcgaaacgc ggtatgcatc gctcccacga ctttctgacc agcccgccgc
     8521 ttgccgtcga gcccaccact ggcgaagtgc atctgcgcca tcacgtcagc cccaacggct
     8581 attaccgcgg caagaaagtt ctcaagacca agggcgactg accgaccccg gcttttggcg
     8641 gcgaggccgc gcagctggat cgaatgagtc gaacgtccgc ttcgacccgc tacagcgggt
     8701 gcaacaaccg cccgagcgcg tatcctgcat ctccccgctg cggcgcgcta gccgaggcaa
     8761 cgcataatcc atggcgatca cgatagcgct cgactgcatg ggtggcgacc acggcgccca
     8821 cgttaccgtg cccgccgccc tcgattttgt cgaccgcgtc gatgacgcgc aggtcgtcct
     8881 ggtgggaatc cccgagacaa tcgaaaccga gctgaagcgg cgccgcgcaa agacaggcga
     8941 gcgcattcgc atcgaagctg caaccgaagt ggtggcaatg gacgaagcgc cggcctcggc
     9001 gctgcgggga aaacgcgact cgtcgatgcg gctcgcgatc gacctggtca aaatcgaacg
     9061 ggcggatgcc tgcgtaagcg ccggcaacac cggcgccctt atggcgatgt cgcggttcgt
     9121 gctcaagatg tcgcccggca tcgagcgtcc ggccatcgcc tctttcctgc cgacccagcg
     9181 tggccgcacc tgcgtgcttg atctcggcgc taatgtggac tgcagcgcgg aacatctgct
     9241 gcagttcggc gcgatgggat cgtcgctggt gagcgctgtg gaacgtgtcg agcgtccgac
     9301 ggtgggcctg ctcaacatcg gtgaagagga aatcaagggg aacgaagtgg tc
//
LOCUS       DASQOP010000219         2862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2115624, whole genome shotgun sequence.
ACCESSION   DASQOP010000219 DASQOP010000000
VERSION     DASQOP010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2115624"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VE030_08960"
     CDS             complement(<1..752)
                     /locus_tag="VE030_08960"
                     /inference="COORDINATES: protein motif:HMM:NF017932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HZF23580.1"
                     /translation="MQTTALMFGFRQWRRQRILRGGGVDDATWQRAVNRFSFAMRLTG
                     QERKQLRDLVVLFLHEKQISAAGGLVLDREMKLGIAIQACILVLNLGIDYYDGWVEII
                     VYPDEFVPRHEFRNEHGLVETDETSYAGQAWLHGPVILSWADVENTGDADGVNVVIHE
                     FAHKLDMLNGNANGFPPLHAGMSREAWAQAFTEAYKDLCRRVKAGEDTDIDPYATEAP
                     GEFFAVVSEAFFEMPDIVRETYPAVYAQLAAF"
     gene            complement(753..2198)
                     /gene="rmuC"
                     /locus_tag="VE030_08965"
     CDS             complement(753..2198)
                     /gene="rmuC"
                     /locus_tag="VE030_08965"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HZF23581.1"
                     /translation="MPELSLALIVSAATLLAVIVLILIVFSMARRQVSHPESLARVLE
                     EKHLAMLKDLNSGLNALGDRLSASQAELSERLRNTVSQELIQTRTALATLQLKQAEEL
                     SANRETLTQRLAQMNTELQGKHDQLRTEVLTRILQTLAEQNRAEQELIQTTMRNATAQ
                     LAASMELLSKTTDTRLEQISGRVSERLEEGFKKTNETFVSVMERLATIDEAQKKLDGL
                     TTNVVSLQELLGDKRSRGAFGEVQLENLVRNLLPEIAYEFQYTFKSNGARADCVLKLP
                     EPTGMVAVDSKFPMENYHRMFEPGVSEADRAVAQRQFRADVKKHVEDIASKYIIPGET
                     SDGAVMFVPAEAVFAEIHAYHPEVVDFAMQRRVWIVSPTTLMAVLNTARAVMKDVATR
                     EQVHIIKDELSKLGKDFARFDQRMKKLADHIRMAHQDAEDVRVSSDKITRRFSQIERV
                     ELDQLEQVREFESALLDSPQDDENGPGETRS"
     gene            complement(2382..>2862)
                     /locus_tag="VE030_08970"
     CDS             complement(2382..>2862)
                     /locus_tag="VE030_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HZF23582.1"
                     /translation="GFQGEFLTITRGMGLASHIFDDYGPVKGDMEERRNGVLISQDVG
                     EAVAYALWKLQDRGRMFVSPGDALYEGMVIGIHSRDNDLVVNPIKGKQLTNVRASGTD
                     EAVRLVPPIELTLEKAVEFIADDELVEITPKSIRIRKRHLKEHERKRASRDSTPAAA"
BASE COUNT          495 a          889 c          890 g          588 t
ORIGIN      
        1 tagaacgccg ccagttgcgc atacaccgca ggataggttt cgcgcacgat gtcgggcatc
       61 tcgaagaagg cctcgctcac caccgcgaag aattctcccg gcgcttcggt tgcatacgga
      121 tcgatgtcgg tgtcttcgcc ggctttcacg cgccggcaga gatccttata ggcctcggtg
      181 aacgcctgcg cccacgcctc gcggctcatg ccggcgtgca gcggcggaaa gccgttggca
      241 ttgccgttga gcatgtcgag cttgtgcgcg aactcgtgga tcaccacgtt gaccccgtcc
      301 gcatcgccgg tattttcgac gtccgcccag gagaggatca ccggcccgtg cagccaggcc
      361 tggccggcat acgaggtttc gtcggtctca accagtccgt gttcgttgcg gaattcgtgg
      421 cgcgggacga actcatccgg atagacgatg atttccaccc agccgtcgta gtagtcgatg
      481 cccaggttga gcaccaggat gcaggcctgg atcgcgatgc cgagcttcat ctcccggtcc
      541 agcaccaggc cccccgcggc ggaaatctgc ttctcgtgca gaaacaacac caccagatcg
      601 cgcaactgct tgcgctcctg tccggtgagc cgcattgcga aggaaaagcg gttgaccgcg
      661 cgttgccagg tcgcgtcgtc caccccgccg ccgcggagaa tgcgctgcct gcgccactgc
      721 ctgaaaccga acatcagagc cgtggtctgc actcaggaac gggtttcgcc gggcccattt
      781 tcatcgtcct gcggcgagtc gagcaaggca ctctcgaact cgcggacctg ttcgagctga
      841 tcgagctcga cgcgctcgat ctgggaaaag cggcgggtga tcttgtcgct tgagacgcga
      901 acgtcctcgg catcctggtg ggccatgcgg atgtggtctg cgagtttctt catgcgctgg
      961 tcgaaccggg cgaagtcctt gcccagcttg ctcagttcgt ccttgatgat gtggacctgc
     1021 tcccgagtgg cgacgtcctt catcaccgcc cgcgcggtgt tcagcaccgc catcagggtg
     1081 gtcggcgaga caatccagac gcgccgctgc atggcgaaat ccacgacctc cggatgatag
     1141 gcgtggattt ccgcgaacac tgcttccgcc ggcacgaaca tgaccgctcc gtcggaagtc
     1201 tcgccgggga tgatgtattt gctggcaatg tcctcgacat gcttcttcac gtcggcgcgg
     1261 aactgccgct gcgctaccgc gcggtccgct tcggaaacgc cgggctcgaa catgcggtgg
     1321 taattctcca tcggaaactt agaatccacc gctaccatgc cggtcggctc gggaagcttc
     1381 agcacgcagt cggcgcgcgc gccgttcgat ttgaaggtgt actggaattc gtaggcgatt
     1441 tcgggaagga ggttgcgcac caggttctcg agttgcactt cgccgaaagc gccgcgcgaa
     1501 cgcttgtcgc ccagcagttc ctgcaggctc accacgttgg tcgtcagtcc gtccagcttc
     1561 ttctgcgcct cgtctatggt ggcaaggcgt tccatcacgc tgacgaaagt ctcgttggtt
     1621 ttcttgaaac cttcttccag gcgctcgctg acgcgaccgc tgatctgctc caggcgggtg
     1681 tcggtggtct tggaaagcag ttccatcgac gccgcaagct gcgcggtagc gttgcgcatc
     1741 gtggtctgga tcagctcctg ctcggcgcgg ttctgttccg ccagcgtctg caggatccgg
     1801 gtgagcactt cggtgcggag ctgatcgtgc ttgccctgaa gttcggtatt catttgcgcg
     1861 aggcgctgcg tgagggtttc gcggttggcg gacagctcct cggcttgctt gagctgcagg
     1921 gtggcaagcg cggtcctggt ctggatcagc tcctgggaaa cggtgttgcg cagcctctcg
     1981 gaaagttcgg cctgcgaggc ggaaagccgg tcgccgagcg cgttgagccc ggagttcaga
     2041 tccttaagca tcgccaggtg tttctcttcc agcacgcggg cgagcgattc aggatggctc
     2101 acctggcggc gcgccatcga gaaaacgatg aggatcagga cgatcaccgc gagcagcgtc
     2161 gcggctgaaa cgatcaacgc aagcgaaagt tcaggcatca agagagtata aaccaagcat
     2221 caccccctct ccccggccct ctcccgccag gggagaggga gcttcaatcc tcaaataaca
     2281 tcaatcccca aataaaaaat gggcgaccac ttggtcgccc gtttttcttc caggactccc
     2341 tctcccctgg cgggagaggg ccggggagag ggggagaggg gctacgccgc cgcaggcgtg
     2401 ctgtcgcgcg aagcgcgctt gcgctcgtgt tccttgagat ggcgcttgcg gattcggatg
     2461 gatttgggcg tgatctcaac cagttcgtcg tcggcgatga actccaccgc tttttccagt
     2521 gtgagttcga tcggcggcac caggcgcacg gcttcatcgg taccggacgc gcgcacgttg
     2581 gtcagttgct tgcccttgat cgggttgacg accagatcgt tgtcgcgcga atggatgccg
     2641 atcaccatgc cttcgtacag cgcatcgccc ggcgagacga acatgcggcc gcggtcctgc
     2701 aatttccaca gggcgtaggc caccgcttcg ccaacgtcct ggctgatcag gacgccgttg
     2761 cggcgctctt ccatgtcacc ctttaccggg ccgtagtcgt cgaaaatgtg gctggccagc
     2821 cccatgcctc gcgtgatggt gaggaactcg ccctggaacc cg
//
LOCUS       DASQOP010000220         2188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1111850, whole genome shotgun sequence.
ACCESSION   DASQOP010000220 DASQOP010000000
VERSION     DASQOP010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111850"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="VE030_08975"
     CDS             <1..581
                     /locus_tag="VE030_08975"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HZF23583.1"
                     /translation="PMHKRPVTPEPPAEEPADDEALLRQALKDVAPIADAGKATLRRK
                     PPKPVPVQRLRDDEQVLQDSLSDHIPHEVGLETGDELVFLRKGLSNMVLRKLRRGHWV
                     NQDQLDLHGLRSEEARHLLVAFLNEALAKGWRCVRIVHGKGLRSENREPVLKRKVGNW
                     LAQRDEVLAFVQARPEDGGSGAVVVLLKAKRK"
     gene            complement(729..1067)
                     /locus_tag="VE030_08980"
     CDS             complement(729..1067)
                     /locus_tag="VE030_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HZF23584.1"
                     /translation="MKKIEAVVKPFKLDEVREALSAIGVTGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKVEVVIPDKLTDQAIEAIIKSARTGKIGDGKIFVTTVEQVVRI
                     RTGETDEAAV"
     gene            complement(1169..>2188)
                     /locus_tag="VE030_08985"
     CDS             complement(1169..>2188)
                     /locus_tag="VE030_08985"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003272417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HZF23585.1"
                     /translation="VFCNLVGGQDELVFDGASFVMDAHGKLIHQLPAFEEHLAFVDIE
                     GARPVPGQIVPEPSTDESIYKALCLGVRDYLGKNGFPGALLGLSGGIDSALTMCIAAD
                     ALGRDKVHAVMMPSQFTADMSVEDSRAMVKALGVRYSEIAIKPVFDAYMGALKPAFAD
                     RPFDTTEENLQSRIRGTLLMALSNKFGSIVLTTGNKSEMSTGYATLYGDMAGGFAVLK
                     DITKKYVYRLSRYRNTLSPVIPERVISRAPSAELRAGQVDQDSLPPYDVLDAIVERYV
                     EKDMSIADIIAEGFKRADVERVVRLVHLSEYKRRQAPIGIRITPRGFGKDWRFPITNK
                     YRGGL"
BASE COUNT          412 a          698 c          672 g          406 t
ORIGIN      
        1 aacccatgca caagcgccca gtcacaccgg aaccaccggc cgaggaaccc gcggacgacg
       61 aggcgttact gcgccaagcg ctgaaggacg tcgcgccgat tgcggacgcc ggcaaagcca
      121 cgctacgccg aaagcccccc aagccggtac cggtgcagcg gctgcgcgac gacgaacagg
      181 tcctgcaaga cagcctttcc gatcacattc cgcacgaagt cggcctggag accggtgacg
      241 aactggtatt cctgcgcaag gggctgtcga acatggtcct gcgcaagctg cggcgcggcc
      301 attgggtgaa ccaggatcag ctcgacctgc acggcctgcg cagcgaggaa gcgcgccact
      361 tgctggtggc cttcctcaat gaagcgctgg ccaaaggctg gcgctgcgtg cgcatcgtgc
      421 acggtaaggg cctgcgctcg gaaaatcgcg aaccggtgct gaaaagaaaa gtgggaaact
      481 ggctggcgca acgcgatgag gtactcgctt tcgtccaggc ccgccccgag gacggcggca
      541 gcggcgcggt ggtcgtgctg ctcaaggcga aacggaaatg acaagtactg agtgctgggt
      601 gctgggtgct gagtaaagtc aaatctggaa aaatctgagc cgcccgcagg cggctcattc
      661 aaatttcctc ttccggcggc gccgaggctg ttgtcccagc actcagcacg cagtactcag
      721 caccgcatct agactgccgc ctcatccgtc tccccggtgc ggatgcggac gacctgctca
      781 accgtggtga cgaagatctt gccgtcgccg atcttgccgg tgcgggccga tttgatgatc
      841 gcttcgattg cctgatccgt gagcttgtcc ggtatcacca cctcgacttt caccttgggc
      901 aggaagtcca ccacgtactc cgccccgcga tagagctcgg tatggccttt ctgccggccg
      961 aaacccttga cctcggtcac cgtcagcccg gtgaccccga tcgccgacag cgcttcgcgc
     1021 acttcgtcca gcttgaatgg cttgaccacc gcttcgattt ttttcatgac cactctcctg
     1081 taaatccgga cccgtgcgcc gcgccggctc gtggacggca agtatagact ccaattgagg
     1141 aatccggaaa ggttgcggcc ctacgccatc acaatccgcc ccggtatttg ttggtgatcg
     1201 ggaaacgcca gtccttgcca aagccgcgcg gcgtgatgcg gattccgatc ggggcctggc
     1261 ggcgcttgta ttcgctgagg tgcaccagtc tgaccacacg ttcaacatct gcgcgcttga
     1321 agccctcggc aatgatgtcg gctatgctca tgtccttctc gacgtagcgc tcgacgatcg
     1381 cgtcgagcac atcgtagggc ggcaggctgt cctggtccac ctggccggcg cgcagttccg
     1441 cgctcggcgc ccggctgatc acgcgctccg gaatgaccgg ggagagggtg ttgcgatagc
     1501 gcgacagccg gtaaacgtac ttcttggtga tgtccttgag caccgcgaat ccgccggcca
     1561 tgtcgccgta gagcgtggca taaccggtgc tcatctcgct cttgttgccc gtggtgagca
     1621 cgatcgagcc gaacttgttc gacagcgcca tcagcagcgt gccgcggatg cgcgactgca
     1681 gattctcctc ggtggtgtcg aatgggcgat cggcgaacgc cggcttgagc gcgcccatgt
     1741 atgcgtcgaa caccggcttg atggcgatct cgctgtagcg cacgccgagg gccttgacca
     1801 ttgcccgcga gtcttcgacg ctcatgtcgg cggtaaattg cgaaggcatc ataacggcgt
     1861 gcaccttgtc ccggccgagc gcatccgctg cgatgcacat ggtgagcgcc gagtcgattc
     1921 cgccggagag acccagtagt gcacctggga agccgttctt gccgaggtaa tcgcgtacgc
     1981 ccaggcacag cgccttgtaa atgctctcgt ccgtcgaggg ctccggcacg atctgtcccg
     2041 gaaccgggcg agcgccttcg atgtcaacga aggcgagatg ctcctcgaac gccggcaatt
     2101 gatggatgag cttgccgtgc gcatccatga cgaaggacgc gccgtcgaaa accagctcgt
     2161 cctgtccacc caccagattg cagaacac
//
LOCUS       DASQOP010000221         7570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2319275, whole genome shotgun sequence.
ACCESSION   DASQOP010000221 DASQOP010000000
VERSION     DASQOP010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7570
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2319275"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="VE030_08990"
     CDS             <1..1039
                     /locus_tag="VE030_08990"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23586.1"
                     /translation="AAALFSTAFGQTPSVPSDTKNETVVNSVAGTVTVLGNEKQQALI
                     QQYLDGVSSASQRQVLIEATIVEVRLSNNYQAGVDWSRLAASIGQGATFQQQLLGGPP
                     TGAPSGLTIGYTNPNSSVGDVRATIRLLEQFGNTRVLSSPKLMALNNQTALLKVVDNV
                     VYFNIEAQTNTNQTTSTTTFTTTAKTVPVGLVMSVLPQVTDSGTVNLTVRPTISRIQS
                     FVPDPNPSLRAQFTAAGVLTSPAIPNLIPQVQVREMESVLQAGSGQTIILGGLMQDDV
                     RRDRDAVPGLGRLPSPASDLFSFRDEAVSKTELVIFLKPTVISNPSLDSDELKFFQRF
                     LPVVDKTGKNP"
     gene            1036..2310
                     /locus_tag="VE030_08995"
     CDS             1036..2310
                     /locus_tag="VE030_08995"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZF23587.1"
                     /translation="MSLLLQALQKAAKSREDGEPGSGPAAADQLALEPIASEPRLREE
                     MPIHSPTPAQAATVVQASRMPPFDPIGYARDHYMIVFLAAAVLFAIVYGSYVYIQVSN
                     PGLLRSTPAPMTSPLASAPAPAAPVAATGVADSAAKISGMPANKATTAALTAGPVPSL
                     FDDSKTAAQPGTTATAPDRAATTRPRRATKATETTVASETAIVSLADAPVETVVIKTN
                     ETKGISVRRQPATLVPVDPTLMQAYEALQRGDLALARNLYQQVLLAEPRSIDALLGLG
                     AIAWKEGRIEEAGQHYQRVLELEPRNPYAQAGLIAIIGGADPKASESRLRQLIAREPS
                     AFLYFTLGNLYADQGQWPGAEQAYFQAYQLQPDNPDYAFNLAVGLERIGQIRPALDYY
                     RKALDLSFRKGRANFDQNLVIQRVGQLSARVE"
     gene            2330..4036
                     /locus_tag="VE030_09000"
     CDS             2330..4036
                     /locus_tag="VE030_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HZF23588.1"
                     /translation="MAEQRKKLRLGELLVQQGLITNDQLRIALTEQKTQNIPIGRLLV
                     RLGFVTEAVIRDIMARTIGQESIDLSQVLVDAEALRLVPQDFARRHRLLPIAFDAEQN
                     VMVVAITEVFNVVALDQLRATLGHNIQMKTVLAGQAQLDEYIDQFYGFELSVDGILRE
                     IETGEIDYQSMQQVGMDEYTQPIVRLVGALLVDAVKRGSSDIHFEPEFAFLRIRYRID
                     GVLEQIRSLHKNYWPGIAVRLKVISGMNIAETRAPQDGRLSLNLNGRPVDFRVSSQPT
                     IWGENIVLRVLDREKSIIGLEQMGLPKSVMAKLNLMLARPEGILVLTGPTGSGKTTTL
                     YSLLAKLNVESVNIMTLEDPVEYPVAMMRQTSVNETAKMDFGNGIRSIMRQDPDIILV
                     GEIRDRDTAEMAFRAAMTGHQVFTTLHTNSALGTFPRLLDIGIAPDIMAGNIIGIIAQ
                     RLVRLLCQHCKEAYTPSPEERLLLGGDIDAVLKIFRPVGCPRCNHRGYRGRTAIMEIL
                     HMDSDLDELVARRASPRELREAANTKNFRSLADEGVHKVVDGTTSLAEVARAIDLTGR
                     VQ"
     gene            4112..5317
                     /locus_tag="VE030_09005"
     CDS             4112..5317
                     /locus_tag="VE030_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZF23589.1"
                     /translation="MPAFEYKAVDRAGRSARGKIDAANEVDLELRLKKMGLDLITSRS
                     LRSHTPTLASGAITRQDLITFCFDLEQVSRAGIPILEGLRDLRDGMENPRFREILTSV
                     LEDIEGGKTLSQALAAFPRVFSAVFASLIRAGELSGTLTEVFENLGGTLKWQDELISQ
                     TRRLLVYPALVFVVVLGVLLFLLLYLVPQVTTLLKTMGLALPLQTRILIGLSNFTAAY
                     WPILLFLPVVIAIGISVIVRTNPRGRYWLDYAILRLPVTGAILQKIILARFANFFALM
                     YRSGITILEAVKTSEDIVANRVIAEGLQRAGQQINAGESLSEAFRNLGMFPPLVIRML
                     RVGESTGALDIALMNITYFYNRDVRDSVDKALKLLEPALTLVLGGMLALILFSVLTPI
                     YDIIGKIRV"
     gene            5324..6877
                     /locus_tag="VE030_09010"
     CDS             5324..6877
                     /locus_tag="VE030_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23590.1"
                     /translation="MAAKLLLCISADQATVASWRGRRLAGCKRFSNDETGWNAFSNFL
                     RAARGIPIHIMVDSVDEDYRFETLPHATGRDRAQMVGRKLKQLYRGTSYYSYSLQERS
                     AGKRKDDRYLFAALTNPELLSPWLHAIDVNGLPVAGVYPLPMVTTSLIERLRLKHANL
                     LIVTKNTAGVRQTFFKDLKFRISRLTPLRDAADVADQYHADEVSNTRMYLDALTVTHV
                     DDMLQIVILDQNDSLAGLPAAVARGRPNMQCHLLTRADIMSRFGIAATELDASADTLH
                     LQLLGEGKPAINFAPRHVTGGFQRYLGRRWVYGASAATLAGALLWSGVNTFEVMRLNE
                     LTENLRRQTQDYQARYQQVTAQFPQAPTTADNLRQTVELAEQIRSSLRTPESMFLVVS
                     HALDALPQIQLTRIAWHYGSLPAELEAGRSQPTTKGHAALESGTRVQSAIMRGEIRPF
                     NGDFKAAMGLINAFAAQLAADGRVAEVRAVRLPLNVSSDAGLSGSTIATGERNSAEFE
                     FAIIFKAGV"
     gene            6884..7495
                     /locus_tag="VE030_09015"
     CDS             6884..7495
                     /locus_tag="VE030_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23591.1"
                     /translation="MKLTSKDLVAMKLPLLVLAISIAASVALVRFSSSKREGAELQHR
                     AQITALQDARSRYQRSGEERETILRYLPAYQQLEKEGFVGTEQRINWLESLRAANEHA
                     ALFGVSYQLDAKKPFPLIGQNNSMSQQLQHSQMKLSFGLVHEGDLMRFLRVLEAQQTG
                     IFILNGCQLDRGGSAGAPAPKQANLNAQCELSWLTIDPGKGKS"
BASE COUNT         1523 a         2373 c         2280 g         1394 t
ORIGIN      
        1 tgccgccgcg ttgttcagca ccgccttcgg gcaaactccc agcgtccctt ccgataccaa
       61 gaacgagacc gtggtcaact ccgtcgccgg cacggtcacc gtgctcggca atgaaaagca
      121 gcaggcgctg attcaacaat acctggacgg cgtcagttcg gcatcgcagc ggcaagtgct
      181 gatcgaagcc actatcgtcg aagtccggct ctccaataat tatcaggccg gcgtggattg
      241 gagccggctg gctgcctcga tcggccaggg cgctacattt cagcagcaat tgctcggcgg
      301 tccgccgacg ggagctccca gcggcctgac gatcggctac accaatccca actccagcgt
      361 gggcgatgtc agagcgacca tccgcctgct cgagcaattc ggcaacacgc gggtgctgtc
      421 gagcccgaag ctgatggcgc tcaataacca gactgcgctg cttaaagtgg tggacaacgt
      481 cgtctacttc aatatcgaag cgcagaccaa caccaatcaa accacttcca ccaccacttt
      541 caccacaacc gcgaagacgg ttccggtggg actggtgatg agcgtcctgc cgcaggtcac
      601 cgattcggga acggtaaatc tgacggtgag accgacgatc tcgcgcattc agagttttgt
      661 tccggacccg aatccttcac tgcgggctca gttcaccgct gcgggagtcc tgacctcccc
      721 cgcgatacca aacctgatcc cgcaagtcca ggtcagggaa atggagtcgg tgctgcaggc
      781 cggcagcggc cagaccatca tactgggcgg tctgatgcag gatgacgtgc ggcgcgaccg
      841 cgatgccgtg cccggcctgg ggcggctgcc ctccccggcg agcgacttat tcagtttccg
      901 cgacgaagca gtgagcaaga ccgaactggt gatcttcctc aagccgacgg tgatcagcaa
      961 tccgtccctg gacagcgacg agttgaagtt cttccagcgt tttctcccgg ttgtggacaa
     1021 gaccggcaag aacccatgag ccttttgctc caggccctgc aaaaagccgc caagagccgc
     1081 gaagacggtg agccgggttc aggacccgcc gcggcagatc agctcgccct cgagccgatc
     1141 gcgtccgaac cgagactgcg tgaagagatg ccgatccatt cgccgactcc ggcgcaggcg
     1201 gctacggtag tgcaggcgag ccgcatgccg ccgttcgatc cgatcggtta tgcgcgcgat
     1261 cactacatga tcgtttttct cgcggcagcc gtactgttcg cgatcgttta cggcagctac
     1321 gtctacatcc aggtatcgaa cccggggttg ctgcgctcaa ctccggcacc gatgacctca
     1381 ccgctcgcgt cggcaccggc gccagctgcg cccgtggcag cgacgggcgt ggcggatagc
     1441 gccgcgaaga tttccgggat gcccgccaat aaagcaacaa cggcggcact gaccgccggg
     1501 cccgtgcctt cattgttcga cgattcgaag acagcggcac agcccggcac gacggcaacg
     1561 gcgcccgacc gggcggcaac gacgcgcccg cggcgtgcca ccaaggcaac ggagacaacg
     1621 gtggcgtcgg aaacggcgat cgtctccttg gccgatgcgc cggttgaaac cgtggtgatc
     1681 aagaccaacg aaaccaaggg catatcagtg cgccgccagc ctgctacgct ggtgccggtg
     1741 gatccgaccc tgatgcaggc ctacgaggcg ctgcagcggg gcgatctggc gctggcgcgc
     1801 aatctctatc agcaagtcct gctggccgag ccgaggagca tcgacgccct gctgggcctg
     1861 ggcgccatcg cctggaagga gggccggatc gaggaagcgg gccaacacta ccagcgggtg
     1921 ttggaactgg agccgcgcaa cccatatgcc caggccggtc tgatcgcgat catcggcggt
     1981 gccgacccga aggcaagcga gtcccggctc aggcaattga tcgcccgcga gccctcggcc
     2041 ttcctctatt tcaccctcgg caacctctac gccgaccagg gccagtggcc gggagcggaa
     2101 caggcttact tccaggctta tcagctgcag ccggacaacc ccgattacgc gttcaatctc
     2161 gccgtcggcc tcgaacgcat cggacagatc cgacccgcgc tcgattacta ccgcaaggcg
     2221 ctcgatctgt ccttccgcaa aggccgcgcc aacttcgacc aaaatctcgt catccagcgc
     2281 gttggccagc tctccgcccg cgtagagtag gtggcgaggt agcgcgacga tggccgagca
     2341 gcgcaaaaag cttcgcttag gcgagctgct ggtccagcag gggctgatca ccaacgatca
     2401 gttgcgcatc gcgctcaccg agcagaagac ccagaacatt ccaatcggcc ggctgctggt
     2461 gcggctgggc ttcgtcaccg aggcggtgat tcgcgacatc atggcgcgga ccatcggcca
     2521 ggagagcatc gacctgtccc aagtgctggt ggatgccgaa gcgttgaggc tggtgccgca
     2581 ggacttcgcc cgccgccacc ggctgctacc gatcgcgttc gatgcggaac agaacgtgat
     2641 ggtggtggcc attaccgagg tgttcaacgt agtggcgctt gatcagctgc gcgccaccct
     2701 cggccacaac atccagatga agacggtact cgcgggccag gcccagctcg acgagtacat
     2761 cgaccagttc tacggcttcg agctgtcggt cgacggcatc ctgcgcgaga tcgagaccgg
     2821 cgagatcgac tatcagagca tgcagcaagt tggcatggat gagtacaccc agccgatcgt
     2881 gcgtctggtc ggcgcactgc tggtggacgc cgtcaagcgc ggttcttccg acatccactt
     2941 cgagcccgaa ttcgccttcc tgcgcatccg ctatcgcatc gatggcgtgc ttgagcagat
     3001 ccgcagcctg cacaagaact actggcccgg gatcgcggtg cgtctgaaag tcatcagcgg
     3061 gatgaacatc gccgagacgc gcgcaccgca ggacgggcgg ctgtcgctga acctgaacgg
     3121 ccggccagtg gattttcgcg tgtcttctca gcccacaata tggggcgaga acatcgtgct
     3181 gcgtgtgctc gaccgcgaga aatcgatcat cggtctcgag cagatgggtt tgccgaagtc
     3241 ggtcatggcg aagctcaact tgatgctggc caggccggaa ggcattctgg tgctgaccgg
     3301 cccgaccggc agcggcaaga ccaccacgct ctattccctg ctcgccaaac tcaatgtcga
     3361 gtcagtgaat atcatgaccc tcgaggatcc ggtcgagtat ccggtagcga tgatgcgcca
     3421 gacctcggtg aacgaaacgg caaagatgga tttcggcaac ggaatccgct cgatcatgcg
     3481 ccaggacccg gacatcattc tggtcgggga aattcgcgac cgcgataccg cggaaatggc
     3541 gttccgggca gcaatgaccg gccaccaggt gttcaccacg ctgcacacca attcggcgtt
     3601 ggggactttc ccgcgcctgc tcgacatcgg catcgcgccg gacatcatgg ctggcaacat
     3661 tatcggcatc attgcgcagc gactggtgcg ccttctgtgc cagcattgca aggaagctta
     3721 cacgccgtcc ccggaagaac gactgttgct cggcggggat atcgatgccg tcctcaagat
     3781 ctttcgcccg gtgggctgtc cccgctgcaa tcaccggggc tatcggggcc gcaccgcaat
     3841 catggagata ctgcacatgg acagcgatct ggacgaactg gtcgcgcgcc gggcctcacc
     3901 gcgggagttg cgtgaggcgg ccaataccaa gaatttccgc tcgctcgctg acgagggggt
     3961 acataaggtg gtcgacggga caaccagcct ggcagaagtg gcgcgcgcca tcgaccttac
     4021 cggacgggtg cagtgagatg ctgcgcatga cacccgcgtt gcgccgcgca cgacgggtgt
     4081 tccgaaatta cggcgtgaca ccgaggcact gatgccagca ttcgagtaca aggcggtaga
     4141 cagagccggg cggtccgccc gcggcaagat cgacgccgcc aacgaagtcg acctcgagtt
     4201 gcggctcaag aagatggggc tcgacctgat cacctcccgg tcgctgcgca gccatacgcc
     4261 cacgctcgcc tccggggcca tcacccgcca ggatctgatt actttctgct ttgatctgga
     4321 acaggtctcg cgcgccggaa tcccgatcct tgaggggctt cgcgatctgc gcgacggcat
     4381 ggagaacccg cgcttccgcg aaatcctgac ttcagtgctc gaggacatcg aaggcggcaa
     4441 gactttgtcc caggcgctgg cggccttccc gcgggtgttc agcgccgtat tcgccagcct
     4501 gattcgcgct ggcgaactat cgggtaccct taccgaggta ttcgagaacc tcggcggaac
     4561 gctcaagtgg caggatgaac tgatttcgca gacgcgtcgt ttgttggtct atccagctct
     4621 ggtattcgtc gtggtactgg gcgtgctgct gttcctgctc ctctacctgg taccgcaagt
     4681 caccaccctg ctgaaaacca tgggcctcgc gctgcctctg cagaccagga tactgatcgg
     4741 gctgtccaat ttcaccgctg cttactggcc gatcctgctg ttcctaccgg tcgtgatcgc
     4801 catcggtatc agtgtgatag tacgtaccaa tccgcgagga cgctactggc tggactatgc
     4861 gatcctgcgc ctgccagtga cgggcgcaat tctgcagaag atcatcctgg cccgcttcgc
     4921 caacttcttt gcgctgatgt accgctccgg cattaccatc ctggaagcgg tcaaaaccag
     4981 cgaggatatc gtcgccaacc gcgttattgc ggaaggcctg caacgcgccg ggcaacagat
     5041 caacgccggc gaaagccttt ctgaagcatt ccggaatctc ggcatgtttc cgccgttggt
     5101 gatacgcatg ctgcgcgtcg gggaaagcac cggcgcgctc gacattgcgt tgatgaacat
     5161 cacttacttc tacaaccggg acgtccgcga ttcggtggac aaggcgctca agctgctcga
     5221 accggcgctt acgctggtac tgggcggcat gcttgctctg atcctgttct cagtgctgac
     5281 cccgatatac gatatcatcg ggaaaattcg ggtctgacag acgatggcag caaagctcct
     5341 gctctgtata tccgccgatc aagccacagt tgcttcatgg cgagggcgcc gtcttgccgg
     5401 atgcaagcgg ttcagcaacg acgagaccgg ctggaatgcg ttcagcaatt tcctccgcgc
     5461 cgcccgaggc ataccgatac acatcatggt cgactcggtg gatgaggatt accgtttcga
     5521 aacgctgccg cacgccacgg gccgcgaccg cgcgcaaatg gttggccgga aactgaagca
     5581 actctaccgc ggcacctcgt attactctta ttcgctgcag gaacgcagcg ccggtaagcg
     5641 caaagacgac cgttacttgt tcgctgcact caccaatccc gaactgttga gtccctggtt
     5701 gcacgccatt gatgtcaacg gcctgcctgt cgccggggtc tatccgctgc cgatggtgac
     5761 cacttcgctc atcgagcgct tgcggttgaa gcacgccaac ctgctgattg tcaccaagaa
     5821 cacagcgggt gtgcggcaga cattcttcaa ggacctcaag tttcgcatca gccgcctgac
     5881 gccattgcgt gatgcggccg atgtcgcgga tcagtaccac gccgacgaag tgagcaacac
     5941 ccggatgtac ctcgacgcgc tgacggtcac gcacgtggac gacatgctgc agatagtgat
     6001 tctcgaccag aacgattcgc tcgcgggctt gccggcagcg gtcgctcgcg gccgtccgaa
     6061 catgcagtgc cacttgctga cccgcgccga catcatgtcc aggttcggaa tcgcagctac
     6121 ggaactcgac gcatcggccg atacgctgca cctgcagctg ctgggcgagg gcaagccggc
     6181 aatcaatttc gcgccgaggc atgtcaccgg cgggtttcag cgctacttgg ggcgccgttg
     6241 ggtgtacggc gcgtcggccg cgacgctggc aggagcgctg ttgtggagcg gcgtcaatac
     6301 cttcgaagtg atgcgtctaa acgagttgac ggaaaacctg cgcaggcaga ctcaggacta
     6361 ccaggctcgg taccagcaag tgactgcgca gtttccgcaa gcaccaacca ctgccgacaa
     6421 cctgcgccag actgtcgaat tggcggagca gataaggtca agcctgcgca caccagagtc
     6481 gatgtttctg gtggtaagcc acgcacttga tgctttgccg cagattcaac ttacgcgtat
     6541 cgcctggcat tacggcagcc tcccggctga actcgaggcc ggccggtccc agccgactac
     6601 aaagggtcac gcggcattgg aatccgggac tcgggtgcag agcgcgatca tgcgcggaga
     6661 gatcaggccg ttcaacggcg acttcaaggc ggcgatgggg cttatcaatg cttttgccgc
     6721 gcaactagcg gccgacggca gggtggccga ggtgcgggcc gtacgcttgc cgctcaacgt
     6781 gagctcggat gcggggttga gcggcagcac cattgccaca ggcgagagaa acagcgccga
     6841 gttcgagttc gccatcatat tcaaagccgg ggtctaggaa tcgatgaaac tgactagcaa
     6901 agatcttgtg gcgatgaaac ttccgttgct ggtgctcgcg atctccatcg cagcctcggt
     6961 cgcgctcgtc aggttctcgt ccagcaaacg tgaaggggcc gagctacagc accgcgctca
     7021 gatcaccgca ttacaggatg cacgtagccg ctaccagcgc tccggcgagg agcgcgagac
     7081 catccttcgt taccttcccg cctatcaaca gctcgagaaa gagggtttcg tcggcaccga
     7141 gcaacggatc aactggcttg agagcctgcg tgcggccaac gaacatgcag cgttattcgg
     7201 cgtgagctat cagcttgacg ccaagaagcc gttccccctg atcggccaga ataattcgat
     7261 gtcgcagcag ttgcaacact cacagatgaa gctgtctttc ggcctggtac atgaaggtga
     7321 tctgatgcgc ttcctgcgcg ttctcgaagc acaacagacc ggcatattca ttcttaacgg
     7381 ctgccagctc gaccgcgggg gcagcgccgg cgcaccggca ccgaaacagg cgaacctgaa
     7441 cgctcaatgc gagctgtcct ggctgactat cgatccggga aagggcaagt cgtgacacac
     7501 ggcttgtgga ttatcctgat gattgcctcg atgctgccgt ggggcatcac gaattctgcc
     7561 gaacccctgg
//
LOCUS       DASQOP010000222         3116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_705108, whole genome shotgun sequence.
ACCESSION   DASQOP010000222 DASQOP010000000
VERSION     DASQOP010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705108"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /gene="prmC"
                     /locus_tag="VE030_09020"
     CDS             complement(<1..615)
                     /gene="prmC"
                     /locus_tag="VE030_09020"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HZF23592.1"
                     /translation="MIATVGALLEKAVRDLVADRDAADARRESQVLLGFALGVSRAWL
                     TTHPDHAADSESAAKFRTLVALRVSGHPVAYLLGVKEFYGLPLRVTPDVLIPRPETEE
                     LVEAALERLPQGEARKVLDLGTGSGCIAIAIARQRPAAKVAAVDSSSPALALARENAA
                     ALDVEAEFLQSNWFAALGRRRFDLIVANPPYVAADDPHLREGDLR"
     gene            complement(612..1709)
                     /gene="prfA"
                     /locus_tag="VE030_09025"
     CDS             complement(612..1709)
                     /gene="prfA"
                     /locus_tag="VE030_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HZF23593.1"
                     /translation="MKPSIASKLAQLSARLEELNALLSGEDVTKNLDNYRKLTREHAE
                     ITPVVELYNAYKVAERDIASALQLAGDPEMRELAEAEVREGKQRLAGIEDQLQRQLLP
                     RDPSDERNIFLEIRAGTGGDESALFAGDLFRMYTRYAERQGWKVDLISESAAELGGYK
                     EVIAKIIGYGAYSKLKFESGGHRVQRVPETETQGRIHTSACTVAVLPEVDAIEDVVLN
                     PAELRIDTFRASGAGGQHINKTDSAVRVTHIPTGIAVECQDDRSQHKNKAQALAVLAA
                     RIKDKQLREQQAKQAATRKSLIGSGDRSERIRTYNFPQGRVTDHRINLTLYKIDQIMD
                     GALDELIGALMAEHQAELLAAMADDLVATQT"
     gene            complement(1706..2959)
                     /gene="hemA"
                     /locus_tag="VE030_09030"
     CDS             complement(1706..2959)
                     /gene="hemA"
                     /locus_tag="VE030_09030"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HZF23594.1"
                     /translation="MQLFAFGVNHHTAPLAVREQVTFHAANLEEALRDLVNREPVREA
                     AIISTCNRTEVYCSTPEPDRAIDWLARFHHLESQSLEPFLYMLPRERAVAHAFRVASG
                     LDSMVLGEPQILGQMKDAVKSAEHAGTLGTVLHKLFQSTFSVAKEVRTQTEIGTNSVS
                     MAAAAVKVAERIFPSVGEQKVLFIGAGEMIELCAAHFAAKQPRYTTFANRTLERGEEL
                     AARYQGQAITLNDMPEQLAMHDIVVSCTASQLPILGKGMIERAIKARRHRPILMIDLA
                     VPRDVEAEVAELDDVFLYSVDDLGKIVQEGLDTRQAAVAQAEAIINASVHDFMHWFET
                     RELVPTIRALRDQAERMRRHEFERAAKLLAKGEDPQKVLEQLSHALTNKFMHAPTHAL
                     NSAEARERDSLVAALARLYQVNPPE"
BASE COUNT          529 a          962 c          999 g          626 t
ORIGIN      
        1 gcgcaggtcg ccctctcgaa ggtgtgggtc gtctgcggca acgtacggcg ggtttgccac
       61 gatgaggtcg aagcggcggc gtcccaatgc ggcgaaccag ttgctttgca gaaactccgc
      121 ctctacgtcg agggcggctg cgttctcgcg cgccaatgcc aacgcgggac tcgaactgtc
      181 caccgcggca acctttgccg caggccgttg gcgcgcgatg gcgatggcga tgcatccgct
      241 gccggtgccg agatcaagca ccttgcgcgc ttcaccctgc ggcagcctct ccagcgccgc
      301 ttccaccaac tcctcggttt cgggccgggg gatgagaacg tcgggcgtga cccgcaacgg
      361 cagcccgtag aattccttca caccgagcaa gtaagccacc gggtgaccac tcacccggag
      421 tgccaccaga gtgcggaatt ttgcagcgga ttcggaatcc gccgcgtggt ccgggtgcgt
      481 agtgagccag gcgcgcgata cgcccagcgc gaaacccaac agcacctgcg attcgcgccg
      541 cgcgtcggcc gcgtcccggt ctgcgaccag gtcccgcacc gctttctcga gaagcgcgcc
      601 gaccgttgca atcacgtttg ggtcgcgacc aggtcatctg ccattgcggc aagcagttcg
      661 gcctgatgct cggccatcaa ggcgccgatc aactcgtcga gcgcaccgtc catgatctga
      721 tcgatcttgt acagcgtgag gttgatacgg tgatcggtaa cgcgtccctg agggaagttg
      781 taggtgcgga tgcgttcgga gcggtcgccg ctgccgatca gcgacttgcg ggtcgctgcc
      841 tgttttgcct gctgctcgcg caactgcttg tccttgatgc gcgcagccag cacggcaagc
      901 gcctgagcct tgttcttgtg ctgggaacgg tcgtcctggc attcgaccgc gatgccggtc
      961 gggatgtggg ttacgcgcac ggcggaatcg gtcttgttga tgtgctggcc gcccgcgccg
     1021 gaagcgcgga aggtgtcgat gcgcaattcc gcgggattaa gcaccacgtc ctcgatcgca
     1081 tcgacttccg gcagcacggc cacagtgcag gcgctggtgt ggatgcgccc ctgggtctcg
     1141 gtctcgggca cgcgctgcac gcgatggccg ccggactcga acttgagctt cgagtacgcg
     1201 ccgtaaccga tgatcttggc gatcacctcc ttgtagccgc ccagttcggc tgcgctctcc
     1261 gaaatcagat ccactttcca gccctggcgt tcggcgtagc gcgtatacat gcggaacagg
     1321 tcgccagcga acagcgccga ttcgtcgccg ccggtgccgg cgcggatttc caggaagatg
     1381 ttgcgttcgt cgctcgggtc cctgggcagc agttgccttt gcagctggtc ttcgatgccc
     1441 gcgagcctct gtttgccttc gcgtacttcg gcttcagcca gttcgcgcat ttccgggtcg
     1501 ccggccaact gcaatgccga ggcgatgtcc cgctcggcta ccttgtaggc gttgtagagc
     1561 tcgaccaccg gcgtgatctc ggcatgctcg cgggtgagct tgcggtaatt gtcgagattc
     1621 ttcgtaacgt cttcgccgga gagcaaagcg ttgagttcct ccagcctggc ggacaattgc
     1681 gccagcttgg aagcaatcga gggtttcatt ccggcggatt gacctggtaa aggcgggcga
     1741 gcgccgcaac cagggaatcg cgctccctgg cttcggctga attgagcgcg tgcgtcggtg
     1801 catgcatgaa tttgttggtc agcgcatggg agagttgctc gagcactttc tgcgggtctt
     1861 cgccctttgc cagcagcttc gcggcacgct cgaactcgtg gcggcgcatg cgttcggcct
     1921 gatcgcgcag cgcgcgaatg gtgggcacca gctcgcgggt ctcgaaccag tgcatgaaat
     1981 cgtgaacact ggcattgatg atcgcttccg cctgcgccac cgccgcctgc cgggtgtcga
     2041 gaccttcctg aacgatcttg ccgaggtcgt ccacgctgta aagaaacacg tcgtcgagct
     2101 cggccacttc tgcttctacg tcgcgcggca ccgccagatc gatcatcagt atcggccggt
     2161 gccggcgcgc cttgatcgcg cgttcgatca tgcctttgcc gaggatcggc aactggcttg
     2221 cggtacagga cactacgatg tcgtgcattg ccaactgctc gggcatatcg ttgagggtga
     2281 tcgcctgccc ctggtagcgg gcggccagtt cctcgccgcg ttccagcgtg cgattggcaa
     2341 aagtcgtgta gcgcggctgt ttggcggcaa agtgcgcggc acagagttcg atcatttcgc
     2401 cggctccgat gaacaacact ttctgctcgc ctacgctcgg aaaaatgcgt tcggcgacct
     2461 ttaccgcggc ggcggccatc gacaccgagt tagtgccgat ctcggtctgg gtgcgcactt
     2521 ctttcgccac cgagaacgtc gactggaaaa gcttgtgaag cacggtgccg agcgtgccgg
     2581 catgctcggc ggatttgacc gcatctttca tctgcccgag gatctgcggc tcgcccagca
     2641 ccatggaatc cagcccgctc gccacccgga acgcgtgcgc gactgcgcgc tcgcgtggca
     2701 gcatataaag aaacggctcg agcgattgcg actcgagatg atgaaagcgc gccagccaat
     2761 cgatcgcgcg gtcgggttcc ggggtgctgc agtagacctc agtgcggttg caggtggaaa
     2821 tgatcgcggc ctccctgaca ggctcgcggt tcaccaggtc gcgcagcgct tcttccagat
     2881 tggcggcgtg aaaagtcacc tgctcgcgaa ccgcgagcgg ggccgtgtga tgattgacgc
     2941 cgaaagcaaa aagctgcatc ggaaccctga aacgacaagg gacctgacgg ccccggaaag
     3001 gccgtcaatt ataagccaaa tgggcggctt tcccttggat tcaggggttt ggaacaggct
     3061 ttcgaatgct tcaagaacgt actgcagcag aggccggtta ggttggtggc caaggg
//
LOCUS       DASQOP010000223         3502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_506879, whole genome shotgun sequence.
ACCESSION   DASQOP010000223 DASQOP010000000
VERSION     DASQOP010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506879"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..162
                     /locus_tag="VE030_09035"
     CDS             <1..162
                     /locus_tag="VE030_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23595.1"
                     /translation="GWSPSEELTREIQNHVKVRLAAHEYPRQVRYVDALPLTSTGKII
                     RRELRSLKD"
     gene            354..1109
                     /locus_tag="VE030_09040"
     CDS             354..1109
                     /locus_tag="VE030_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZF23596.1"
                     /translation="MQDQTILETRQLSREFSGFVAVNNVSLKVRQGSIHGLIGPNGAG
                     KTTVFNLLTKFLLPTSGTILYRGEDITGDRPARVARRGLVRSFQISSTFPHMTVLENV
                     RVALQRRLGNSFQFWRSQRSLGQLDERAMDILTSVELAGHAQLQATELPYGRKRALEF
                     ATTLALEPEVMLLDEPTQGMAHEDVGRVVGLIRKVASGRTILMVEHNLSVVEDLCDTI
                     TVLQRGAVLAEGTYREVSANPQVLEAYVGVADE"
     gene            1102..1812
                     /locus_tag="VE030_09045"
     CDS             1102..1812
                     /locus_tag="VE030_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZF23597.1"
                     /translation="MNDGAPILEVADLHAWYGESHVLHGMQFAVHRGELVTLLGRNGA
                     GRTTTLRAILGLTGKRSGSILVRGRQAIALATHRVARLGIGYCPEERGIFASLSAEEN
                     LRLPPRIGAGGMSIEDIYAMFPNLHERRKSPGSRLSGGEQQMLALARILRTGADLLLL
                     DEITEGLAPVLVKTLGRAVRSLKEKGYTIIMVEQNFRFAAPLADRHYVVELGRVVETV
                     NKSELDARLDLLHEYLGV"
     gene            1841..3067
                     /locus_tag="VE030_09050"
     CDS             1841..3067
                     /locus_tag="VE030_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZF23598.1"
                     /translation="MNRTRICALSCAAVFVACAAAAMAQQGPVKISDNVVRIGVLTDM
                     SGLYSDIGGQGSVVAAQMAVDDFGGKVLGKKVDVVATDHQNKADVAAQKVREWIDRGG
                     VDMITDALNSAVALATSKVVAEKKRILMDTGAASTRLTNEDCTRYTIHHTYDTFALAN
                     GTGRAVVKQGGDSWFFLTADYAFGHSLEQDTMNVVKENGGKVLGAVRHPLSASDFSSF
                     LLQAQASKAKIVGLANAGGDTINSIKAANEFGLTKTQTLAGLLVFVSDIHSLGLQATQ
                     GMYVTEAWYWDLNDQTRKWSKRFFDKTKRMPTMIHAGVYSATTMYLNAVKATGTDDAD
                     AVMSWMRASEHSDMFGTNMTLREDGRLVHDMYLFQVKKPSESKGPWDYYHLRETIAAD
                     HAFQPLSKSRCPLINR"
     gene            3390..>3502
                     /locus_tag="VE030_09055"
     CDS             3390..>3502
                     /locus_tag="VE030_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZF23599.1"
                     /translation="MELFGIPIQALLGQLLLGLVNGSFYAMLSLGLAVIFGL"
BASE COUNT          705 a         1051 c         1106 g          640 t
ORIGIN      
        1 ggttggagcc cgagcgagga gctgactcgc gagatccaga accatgtgaa ggtgaggctg
       61 gcggcccacg aatatccgcg ccaggtccgc tatgtcgatg cgctgccgct gaccagcacc
      121 ggcaagatca tccgccgcga gttgcgcagc ctcaaggact gacggtgcac gaccggcatc
      181 gatgcagtaa cctgtcggtc ccctggacca gaactggaaa cgtaaccacc aaggcaccaa
      241 ggaatgcaaa tgggaacttg gaaacattgt cagatcttct tggtgccttt atgtcttggt
      301 ggtcagaatt gattcggcca tcgagttttt caacagcctg ctgacagagc cagatgcaag
      361 accagaccat cctcgaaacc cggcagcttt ccagggagtt cagcggtttc gtggccgtga
      421 acaacgtcag cctcaaagtc cggcagggca gcattcacgg gctgattggc cccaatggcg
      481 cgggcaagac cacggtgttc aatttgttga ccaagttcct gctgccgacc tccggcacca
      541 tcctctaccg cggcgaggac atcaccggcg atcgtcccgc gcgggtggcg cgacgcggac
      601 tggtacgctc cttccagatc tcttcgacct tcccccacat gacggtgctc gagaacgtac
      661 gggtggcgct tcagcgcagg ctgggcaatt cgtttcagtt ctggcgttcg cagcgctcgc
      721 tcggacaact cgatgagcgc gcgatggaca tcctcaccag cgtcgaactc gccggacacg
      781 cgcagctcca ggccaccgag cttccctacg ggcgcaaacg cgcgctggag ttcgccacca
      841 cgctggcgct ggaaccggaa gtgatgctgc tcgatgagcc gacgcaggga atggcgcacg
      901 aggacgtggg ccgcgtggtc gggctgatcc gcaaagtcgc ctccgggcgc accatcctga
      961 tggtcgagca caacctgagc gtcgtggagg atctgtgcga caccatcacg gtgctgcaac
     1021 ggggggcggt gctggccgag ggaacttacc gggaagtgtc tgccaatcct caggtgctgg
     1081 aagcctacgt cggggtcgcc gatgaatgac ggcgctccga tcctggaggt cgccgacctg
     1141 cacgcctggt acggcgagtc gcacgtcctg cacggaatgc agttcgccgt gcaccgcggc
     1201 gagctggtca cgctgctcgg ccgcaacggc gcggggcgca cgaccacgtt gcgcgccata
     1261 ctcggactta ccggcaagcg cagcggctcg atcctggtgc gaggacggca ggcaatcgcc
     1321 ctggcgacgc atcgcgtcgc gcggctgggc atcggctact gcccggagga acgcggcatc
     1381 ttcgccagcc tgtcggccga ggaaaacctg cggcttccgc cgcggatcgg cgccggcggc
     1441 atgagcattg aagacatcta tgccatgttc ccgaatctgc acgaacgcag gaaaagtccc
     1501 ggaagccggc tttccggcgg cgagcagcag atgctggcgc tggcgcggat cctccgcacc
     1561 ggtgcggatc tgctgctgct ggacgaaatc accgaggggc tggctccggt gctggtcaag
     1621 actcttggcc gcgcggtccg cagcctcaaa gagaagggct acacgattat catggtggaa
     1681 cagaatttcc gtttcgcagc tccccttgcc gaccggcatt acgtggtcga gcttgggcgc
     1741 gtggtggaaa cggtcaacaa gagtgagctg gacgcccggc tcgatctgct gcacgagtat
     1801 ctgggcgtct gagcggattg cacatttgga ggagagaaaa atgaaccgaa caaggatctg
     1861 cgccctgtcg tgcgctgctg tgttcgttgc gtgcgcggcc gcggcgatgg cccagcaggg
     1921 gccggtaaag atatccgata acgtggtgag aatcggcgtg ctgaccgata tgtccgggtt
     1981 gtattccgat atcggcgggc agggttcggt ggtcgccgca cagatggcgg tggacgactt
     2041 cggcggcaaa gtgctcggca agaaggtcga tgtggtcgcc accgatcacc agaacaaggc
     2101 tgacgtggcc gcgcagaagg tgcgcgaatg gatcgaccgc ggcggcgtgg acatgatcac
     2161 cgacgcgctc aactcggcgg tggctctggc cacttccaag gtcgttgccg aaaagaagag
     2221 gatcctgatg gataccggcg cggcttcgac ccgtctcacc aacgaggact gcacgcgtta
     2281 caccatccac cacacctacg atacctttgc gcttgccaac ggcaccgggc gcgcggtggt
     2341 gaagcagggc ggggatagct ggttctttct cactgccgac tatgccttcg gccattcgct
     2401 cgagcaggac accatgaacg tggtgaagga aaacggcggc aaggtgctgg gtgccgtacg
     2461 gcacccgttg tccgcgtccg acttttcttc gttcctgctg caggcgcagg cctcgaaagc
     2521 caagatcgtc gggctggcga acgcgggcgg cgacaccatc aattccatca aggctgccaa
     2581 cgaattcggg ttgaccaaga cccagaccct cgcgggcctg ctggtgttcg tcagcgacat
     2641 ccacagcctt ggcctgcagg ccacgcaggg catgtacgtc accgaggcct ggtactggga
     2701 cctgaacgat cagacccgca agtggagcaa gcggttcttc gacaagacca agagaatgcc
     2761 gacgatgatc catgccggcg tgtattcggc gacgaccatg tatctcaacg cggtcaaggc
     2821 gactggcacc gacgatgccg atgccgtgat gtcgtggatg cgtgccagcg agcacagcga
     2881 tatgttcggc acgaacatga cgctgcgcga ggatggccgc ctggtgcacg acatgtacct
     2941 gttccaggtc aagaagccgt ccgagtcgaa gggaccgtgg gactactatc acctgcgcga
     3001 aacgatcgcg gccgaccacg ccttccagcc gctttcgaag tcgcgctgcc cgctgatcaa
     3061 tagatgaggc cgttccggac ggcgtccggt aagtcgggca cctgaaaaaa gcgctcacca
     3121 caaataagcg ctcaccacgg aggacgcgga ggacacggag gaaaggcaag agcaagaaaa
     3181 taagttcggg taaaaaccgt tcattggctc atgatccatc gcagcgaaaa ttggccgtac
     3241 aggttagcga acagttaccc cgcttttatc ctcaacttgc ttttcctcgt ttctgttttt
     3301 cctccgtgtc ctccgcgtcc tccgtggtaa gagtttgtct agactcgttg accggataac
     3361 catagaaaag gctgaggaat caaaacctga tggagctctt cggcataccg atccaggcac
     3421 tgctcggcca gcttttgctc ggtctggtca acggctcgtt ctacgcgatg ctgagcctcg
     3481 ggctcgcggt cattttcggc ct
//
LOCUS       DASQOP010000224         4917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1912614, whole genome shotgun sequence.
ACCESSION   DASQOP010000224 DASQOP010000000
VERSION     DASQOP010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4917
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1912614"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..552
                     /locus_tag="VE030_09060"
     CDS             148..552
                     /locus_tag="VE030_09060"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZF23600.1"
                     /translation="MTTAALIQEVLNPLFPGLMGVRLMEVTPERVVASMEVRPDLCTT
                     GNVLHGGAIMAFADTLGAIGTIANLPKDARTTTIESKTNFLRAAPEGTRITGECTAFH
                     RGKTTMVWQTQIKTDTGKLCAVVTQTQLVLPS"
     gene            complement(617..1948)
                     /locus_tag="VE030_09065"
     CDS             complement(617..1948)
                     /locus_tag="VE030_09065"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZF23601.1"
                     /translation="MRPDMIGVYDPELVVLSVVVAAIASYTALDLAGRVSESRGTSSW
                     IWLLGGAFSMGTGIWAMHFIGMLAFHLPVPLAYDVPINVLSWLIAMLVSGVALFVVRR
                     PTMSGGYLSIGAALMGVGICVMHYTGMAAMRMSPPIKYDPALFIASVIIAIAASMAAL
                     WIAFQLRRKQPGTAILAKIGSALLMGFAISGMHYTGMAAAEFAPDSICLAAGSGGGLS
                     NHMLGVVVGAATMSILMITLVISAFDSQIAARAAKHAESLQAANEQLRNIALYDSLTG
                     LPNRLLFEDRMEQAMVRSGRSGRPCALMFVDLDRFKPVNDTFGHRVGDELLKAAAGRL
                     ANCVRKEDTVARAGGDEFIVVLSELTKREDAALIGGKILQQLSRPFSVERHELEISGS
                     IGISVFPEDGKTLLELLNNADVAMYHAKKAGRNGYLFFAPDMGTTRPAPAR"
     gene            2434..3030
                     /locus_tag="VE030_09070"
     CDS             2434..3030
                     /locus_tag="VE030_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23602.1"
                     /translation="MKISTVLTAVCAVLLIAACASKSDKPTRFELRGFSFTAPAAVED
                     EKPWGVAKRTPDLLILGKPGEFQSEVFTVQATVVRLPALASPDALLQYVKSSQQRELD
                     PKRFRILKHELSLQPVNGESCALSHIEAVDRAMRSTTGPTANTMLETLTLTCAHPKDR
                     NTGINVTYSQQYFPEDKDPRFLERGSAILEGLRLDGQP"
     gene            complement(3057..4424)
                     /locus_tag="VE030_09075"
     CDS             complement(3057..4424)
                     /locus_tag="VE030_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZF23603.1"
                     /translation="MNLYAKLQERETNGKRLRVGLIGAGKFGAMYLAQVPKTPGLHLA
                     GIADLSPQNARTNLERVGWKAERYAAPSLDAAISSGLTHVGEDWQALVAHPAVDIIVE
                     ATGNPIAAVEHALAAFSHGKHVVMVTVEADAMCGPLLARRAREASVVYSLAYGDQPAL
                     ICDLVDWARAAGFNVVAAGRGHKWLPHFAQSTPETVWGHYGLTPEQAKIGGLNPKMFN
                     SFLDGSKPAIECTAVCNATGLTPAPWGLKFPPASVDDIPNAMRPVSEGGHLHHKGQVE
                     VISSLEADGRVIPYDIRFGVWVVFEGETEYIRNCFKEYMVRTDSSGRYACLYKRWHLI
                     GLEVGISVASVGLRREPTGCPTGFRADVVATAKRDLKPSEILDGEGGYTVVGRLMQAQ
                     ASLRIGGLPLGLAHGVKLLKPVAAGQPLRWTDVAVDESSHAVKVRREMERLFALHPEA
                     TSLTQ"
     gene            complement(4483..>4917)
                     /locus_tag="VE030_09080"
     CDS             complement(4483..>4917)
                     /locus_tag="VE030_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HZF23604.1"
                     /translation="VNEFNGPLMFIPGSHKLGVLDAQHDTSTTSYPLWTINHDSIRKL
                     VERGGIVAPKGPAGSMILFHSCLVHASSSNLSPWNRVSVYLSLCAVSNHIRRFKRPEY
                     IAHRDFTPIQCLPDDCLLRDYDVPLPWKDGTPEEALKPQLVA"
BASE COUNT         1009 a         1521 c         1470 g          917 t
ORIGIN      
        1 gtgacgggag cgaatctgga ggaagcgaat gttgacggga caatattcaa aggcgtgcgc
       61 gggctggatg ccgcgaaggg cttcgaccgc gccgaacacc gggacaacgc caaattctag
      121 aaagctcgat ctccgggaga aaatgtcatg accacggcag ccttgattca agaagtgttg
      181 aatcccctct ttcccggcct gatgggagtg cggctgatgg aagtcacccc agagcgggtg
      241 gtcgcatcga tggaggtgcg tccggacctg tgcaccacag gcaacgttct acacggtggt
      301 gcaatcatgg ccttcgccga caccttgggg gccattggca ccattgcgaa tctgccgaaa
      361 gatgcgcgca ccaccacgat cgaatcgaag accaacttcc tgcgtgcggc gccggaaggc
      421 acgcgcatca ccggggagtg caccgcgttt caccgcggca agacgacgat ggtgtggcag
      481 acacagatca agaccgacac cggtaagctg tgtgccgtcg tcacccagac acaactggtc
      541 ctcccatcgt gacctgccgg ccaatattac gggcaacgga tcgggctgat acggatgcga
      601 cgcggcgaaa tcctgcctat cgcgcgggag cgggccttgt tgttcccata tccggcgcga
      661 agaacagata gccgttgcgc cctgctttct tggcgtgata catcgccacg tcggcgttgt
      721 tcagcagttc cagcaaggtc tttccgtctt cagggaaaac gctgatcccg atgctgcccg
      781 aaatctccag ctcgtgacgc tcgacggaaa aaggccgcga taattgttga agaatcttcc
      841 cgccgatcag ggcggcgtcc tctcgctttg tcagctcgct cagcacgacg atgaattcgt
      901 ctccgccggc gcgcgccacc gtgtcttctt ttctgacgca gttggcaagg cgtcccgcag
      961 ccgccttcaa tagctcgtca ccaacacggt ggccgaaggt gtcgttgacg ggtttgaacc
     1021 tgtcgagatc gacgaacatc agtgcgcaag gcctgccact gcgtccggaa cgtaccattg
     1081 cctgctccat cctgtcttcg aacaacaagc ggttcggaag gcccgtcaga ctgtcgtaca
     1141 gcgcgatgtt gcgcagttgc tcgttcgcgg cctgcagcga ttccgcatgc ttcgcggcgc
     1201 gggcggcgat ctgcgagtcg aacgcggata tcaccagcgt gatcatcagg atgctcatgg
     1261 tcgccgcacc gacaacgacg ccaagcatgt ggttgctcag accgccgccg gaaccggcgg
     1321 caagacaaat gctgtcaggt gcaaactcgg ccgccgccat tcccgtatag tgcatgccgg
     1381 aaatggcgaa gcccataagc agtgcgctgc cgattttggc caggatggcg gtgccgggct
     1441 gcttgcgccg caactgaaaa gcgatccaaa gagcggccat ggacgctgct atcgcgatga
     1501 ttacggacgc gatgaaaagc gcagggtcat atttgatcgg gggcgacatc cgcatcgccg
     1561 ccatcccggt gtagtgcatg acgcaaatgc cgacacccat caacgcggcg ccgatggaga
     1621 gatacccacc gctcatggtg ggccgccgca ccacaaacag ggcaacgccc gataccagca
     1681 tcgctatgag ccacgacagc acattgatcg ggacatcgta ggccaacggg acaggcaaat
     1741 gaaaagccag catgccgatg aaatgcatcg cccagatgcc ggtgcccatg gagaacgcgc
     1801 cgccaagcag ccagatccac gagcttgtgc ccctggactc gctcacgcgt ccggcaagat
     1861 cgagagccgt ataggacgcg attgccgcga ccaccacgga aagaacgacc agctccgggt
     1921 catacacacc gatcatatcg ggcctcatgt cagcagacag gcacagcctt cgactacctc
     1981 gccctggcac ataatgctca agcgcctttg atgccccatt tcattcaata gcgttgcacg
     2041 ttttccggac ccctgcaaac tttttctaag gtgcaaatca cggggtcaat cccggatatt
     2101 gaacgccgtg aattttaggc ataaccttcg atcgcggacg aacaggatcg aatccgcccg
     2161 cacatccgtt tcttcgtgaa cggggacggc gtcacccgcc tggaccatcg cctgcaggcc
     2221 ggcgacgaag tgatcatcgt tgccgcgctg agcggcggat gaatcaaaac gttaaccacc
     2281 gaggcatcaa gacaccaaag gacgcaaatg gaaacgtgga aactttgtct gtttttcttg
     2341 gtgtctttgt ggcttggtgg taagaattgg ttttggccat cgagtttttc aacaacctat
     2401 tagggcagcc tgcccttttc taacgtgagt taaatgaaaa tttcaaccgt cctgactgct
     2461 gtttgcgcgg tgctcctgat tgccgcatgc gcatcgaaat ccgataaacc gactcgtttt
     2521 gaactgcggg gattctcgtt cactgcaccg gcagcggttg aggacgagaa gccatgggga
     2581 gtcgcaaagc gaacgcccga tcttctgatc ctcggcaaac ccggagaatt ccaaagcgag
     2641 gtctttaccg tgcaggcgac ggtcgtaagg cttccggcac tcgcatctcc cgacgcattg
     2701 ttgcaatacg tcaagtcgtc gcagcagcgc gagctggacc ccaagcgctt ccgcatcctc
     2761 aagcacgagc tcagtttgca accggtgaac ggggagtcct gtgcgctttc gcacatcgag
     2821 gcggtggacc gcgccatgcg cagcaccaca gggcccacgg caaacaccat gctggaaacg
     2881 ctgacgttga cctgcgcgca ccccaaggac cggaacaccg gaatcaacgt tacttactcg
     2941 cagcagtact ttccggaaga caaggacccc cggttcctcg aacggggcag cgccattctc
     3001 gaaggactca ggctggatgg acagccgtag ggaagctcta tccggaaacg tgacaactac
     3061 tgcgtcagcg aggtggcttc cggatgcaat gcgaacagcc gttccatttc cctgcgcacc
     3121 ttgacggcgt ggctggactc gtcgacggca acatcggtcc atcgcagcgg ctggcccgcg
     3181 gcaaccggct tgagcagctt cacgccgtgg gcgagcccga gcggcaggcc gccgatcctg
     3241 agggaagcct gagcctgcat gagtctgccg accaccgtgt agccgccctc gccgtccagg
     3301 atctcgctag gcttcagatc gcgcttggca gtggccacca cgtcggcgcg aaacccggtg
     3361 gggcagccgg ttggctcgcg ccgcaatccc actgacgcaa ccgagatgcc gacctccagg
     3421 ccgatcagat gccagcgctt atacaggcag gcatagcggc ccgatgagtc ggtgcgcacc
     3481 atgtactctt tgaagcagtt gcggatgtat tcggtttcgc cctcgaacac gacccacaca
     3541 ccgaaacgga tatcgtaggg aatcacccgc ccgtccgcct ccagcgagga aatcacctcc
     3601 acctgcccct tgtggtgcag gtggccgcct tcagaaaccg gacgcatcgc gttcggtatg
     3661 tcgtccacgc tggcgggcgg aaacttcagt ccccaaggcg ccggcgtcag gccagtggca
     3721 ttgcacacgg cggtgcactc gatcgccggc ttcgaaccgt cgaggaagga attgaacatc
     3781 ttcggattga ggcccccgat cttggcctgc tccggagtga gcccgtagtg gccccacacc
     3841 gtttccggcg tggactgggc gaaatgcggc aaccacttgt ggccacggcc ggcggcaacg
     3901 acgttgaatc cggcggcgcg cgcccagtcg accaggtcac agatcagcgc cggctgatcg
     3961 ccgtaagcca ggctgtagac cacgctggct tcccgcgccc ggcgcgcgag caatgggccg
     4021 cacatcgcgt ccgcttcgac ggtgaccatc accacgtgct tgccgtgact gaatgcggca
     4081 agtgcgtgct cgaccgcggc aatcggattg ccggtggctt cgacgatgat gtccaccgcg
     4141 gggtgcgcaa cgagcgcctg ccagtcctct cccacgtgcg tgagccctga ggatatcgca
     4201 gcgtcgagcg acggcgcggc gtaacgctcc gccttccagc ctacgcgctc gagattggtc
     4261 ctggcatttt gcggggacag gtcggcaatg ccggccagat gcaggccggg cgtctttggc
     4321 acctgggcaa ggtacatggc accgaacttt ccggcgccga tcagcccgac gcgcagccgc
     4381 ttgccgttcg tttcgcgctc ctgcagtttg gcgtacagat tcatggctcg ttccgttgaa
     4441 ctttcattga actgtcattt ccgattgcgc gggaatgaca atctatgcca caagctgggg
     4501 cttcaatgct tcttccggcg tgccgtcttt ccagggcaaa ggcacgtcgt agtcgcgcag
     4561 caggcagtcg tccggcagac actgtatcgg cgtgaaatcg cggtgcgcga tgtactcggg
     4621 gcgcttgaag cggcggatat ggttggagac cgcgcacagg ctcaggtaca cgctcacccg
     4681 gttccagggc gacaggttgg acgacgaggc atgcaccagg caggagtgga acaggatcat
     4741 cgagccggcc ggccctttgg gtgccacgat tccgccgcgt tccaccagct tgcggatgct
     4801 gtcgtggttg atggtccaca acggatagct cgtggtcgac gtgtcgtgct gcgcgtcgag
     4861 caccccgagc ttgtggctgc cgggaatgaa catcagcggc ccgttgaact cgttcac
//
LOCUS       DASQOP010000225         3300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_302733, whole genome shotgun sequence.
ACCESSION   DASQOP010000225 DASQOP010000000
VERSION     DASQOP010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302733"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VE030_09085"
     CDS             complement(<1..255)
                     /locus_tag="VE030_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZF23605.1"
                     /translation="MNRTLVLNVVGLTPALLAHAPNLSALAKRGGMRPLDTVVPALTT
                     TVQSTFVTGALPREHGIVGNGWYFRDLSEIWLWRQSNRLVG"
     gene            459..1172
                     /locus_tag="VE030_09090"
     CDS             459..1172
                     /locus_tag="VE030_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HZF23606.1"
                     /translation="MTPSSIRALTFDVFGTVVDWRGSIIREGRALGKQKNLKADWAAV
                     ADAWRDGYKPAMDRVRKRQLPWMSIDQLHRLILDDVLAKLRIEGLTPEDKDRFNLAWH
                     RLRPWADARPGLKRLKKRYVLATLSNGNVSLLVDMARKAGLPWDCVLSAELFRHYKPD
                     PEAYLGAAALLGLQPSEVMMVAAHKDDLQAAKRCGLRTAFVRRPMERGTGVKADLSPD
                     RSFDFNADDFNDLASQLEA"
     gene            1182..1874
                     /locus_tag="VE030_09095"
     CDS             1182..1874
                     /locus_tag="VE030_09095"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HZF23607.1"
                     /translation="MKAQDSSKVLETQATTTVWDLPLRIFHWVLVLLVISQVVTATIG
                     GNAMEYHALGGYTILTLVLFRILWGFSGGTHARFGDFLNGPAAVARYAHKLIKGKAET
                     YRGHNPLGGWSVALMLASLSVQAVTGLFANDDVMMEGPLAKHVSDDASALFTAIHDIN
                     AGILLALISLHILAVFFYLFRRKQDLIVPMITGRKPIELMDGRPPRAGNLWLAALLLA
                     ACSLFVYFVVTF"
     gene            complement(1864..2517)
                     /locus_tag="VE030_09100"
     CDS             complement(1864..2517)
                     /locus_tag="VE030_09100"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZF23608.1"
                     /translation="MSDLLSSRTKLGRYPVRGSHDFATIAAILDEGFFCHVGFVVKDQ
                     PYVIPTGFGRDGHTLYIHGSAASRMLNEAGKGVALSVTVTLVDGLVLARSAFHSSVNY
                     RSVVILGTAEPVPAADKLRALKTISDHIIPGRWESLRPVKPQELDQTAVLRMEIIEAS
                     AKVRTGPPLDDEEDYGLPIWAGTLDFAPLSTTPKPDPRLASGIPVPEHAQRYRRVKK"
     gene            complement(2614..3063)
                     /locus_tag="VE030_09105"
     CDS             complement(2614..3063)
                     /locus_tag="VE030_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZF23609.1"
                     /translation="MKRLAAALLFAVCASVFANAAFAQKPEDAIKFRRGVMAVIGWHF
                     GPMAAMAKGDRPYDAAAFARHAEIVAFMSKLPLEGFVPGSDTGETKAKPEIWLDMDDF
                     KAKLEKMQAEVAKLAEVAKAGDLNTSKAQLGETGKACKACHDKYRNK"
BASE COUNT          622 a         1026 c         1019 g          633 t
ORIGIN      
        1 gcccaccagg cggttggact ggcgccacag ccagatctcg gagagatcgc ggaaatacca
       61 gccgttgccg acgatgccgt gttcgcgcgg cagcgcgccg gtgacgaagg tggattgcac
      121 cgtcgtggtc agcgccggca ctaccgtgtc gagcggacgc atgccacccc gcttcgccaa
      181 cgcactcaga ttcggcgcat gtgcaagcaa cgccggcgta aggcccacca cgttcagtac
      241 cagcgtgcgg ttcatggctg cgattgtaac tctgccaagg tgacgaaagg cgagaggaaa
      301 tggggaatgg ggaatgggga atggaccggc atgacccgaa ctaatttctg ccggtcaacc
      361 gggcggacat tttttcccct gcccgcgccg tctataatcc ctgccatgcc tgcttcgcga
      421 ccgtttgcgg catcctgaat aattgagaag aatcgacaat gaccccctcg tcaattcgcg
      481 cgctgacttt cgacgtgttc ggtaccgttg tggactggcg cggcagcatc attcgcgaag
      541 gccgcgcact cggaaagcag aagaacctga aagcagattg ggcagccgtc gcggacgcat
      601 ggcgcgacgg ttacaaaccg gcgatggacc gggtgcgcaa gcggcaattg ccgtggatga
      661 gcatcgatca actgcaccgg ctgatcctcg acgacgtgct ggccaagttg cggatagaag
      721 gtctcactcc ggaggacaag gatcgcttca atctcgcctg gcatcgcctg cgcccatggg
      781 cggacgcgcg accggggctg aagcgcctga agaaacgcta cgtgcttgcc acgttgtcga
      841 acggcaacgt gtcgctgctc gtggacatgg ccaggaaagc gggtctgccg tgggactgcg
      901 tgctttcggc ggaactgttc cggcattaca agcccgatcc cgaagcctat ctcggcgcgg
      961 ctgcgctgct cggcctgcag ccctcggaag tgatgatggt ggccgcgcac aaggacgatc
     1021 tccaggcggc gaaacgttgc gggttgcgta ccgctttcgt gcgccggcca atggaacggg
     1081 gaacgggtgt gaaagccgat ctgtcgccgg accgctcctt cgatttcaat gccgacgact
     1141 tcaacgatct cgcctcgcaa ctcgaagcgt aaagccccgg gatgaaagca caggattcca
     1201 gcaaagtatt ggagacccag gccacgacca ccgtctggga cctgccgctg cgcatcttcc
     1261 actgggtgct ggtgctgctg gtgatctctc aagtcgtcac cgcgacgatc ggcggcaacg
     1321 cgatggaata ccacgcgctt gggggctata cgattctcac gttggtgctg tttcgcatcc
     1381 tgtggggatt ttccggggga acgcatgcgc ggttcggcga cttcctgaac gggccggcag
     1441 ccgtggcgcg ctacgcgcac aagctgatca agggcaaggc cgaaacctat cgcggccaca
     1501 acccgctggg cggctggagt gtcgcgctga tgcttgcgtc actatccgtg caggcggtga
     1561 ccgggctgtt cgccaacgac gacgtgatga tggaagggcc gctggcgaaa cacgtgtcgg
     1621 acgatgccag cgcactgttt acggccatcc acgacatcaa cgccggcatc ctgctggcgc
     1681 tgatttcgct ccacatcctg gcggtatttt tctacctgtt ccgcaggaaa caggacttga
     1741 tcgtgccgat gatcaccggc cgcaaaccga tcgagctgat ggacggaaga ccgcccaggg
     1801 ccggcaacct ctggctcgcc gcgctactcc tcgcggcatg ctcattattc gtttacttcg
     1861 tcgttacttt ttaacccgcc gatagcgctg cgcgtgctcc gggaccggga tccccgatgc
     1921 aaggcgcgga tcgggcttcg gcgtggtaga aagcggcgcg aaatccagcg tgcccgccca
     1981 gatcggcaga ccgtaatcct cctcgtcgtc caacggcggg ccggtgcgca ccttcgcgga
     2041 cgcctcgatg atttccattc tgagcacggc ggtctggtcg agttcctgcg gtttgaccgg
     2101 gcgcaggctc tcccagcgtc ccgggatgat gtgatccgag atcgtcttga gggcgcgcag
     2161 cttgtcggcc gcgggcaccg gctcggcggt gccgaggatc accaccgatc gataattcac
     2221 cgacgaatgg aaggccgagc gcgccagcac cagtccatcc accagcgtca ccgtcacgct
     2281 cagcgcgacg cccttccccg cttcgttcag catcctgctc gccgccgaac cgtggatgta
     2341 aagcgtatgg ccgtcgcgcc cgaaacccgt ggggatgacg tagggctggt ccttgacgac
     2401 gaacccgaca tggcagaaga aaccctcgtc gaggatcgcc gcgatggtcg cgaagtcgtg
     2461 gctgccgcgc accggatagc ggccgagttt agtgcggctg gagagaagat cggacataag
     2521 atcaggggag agggacgagg atcgagggac gagggaaatt ccaagattcg aaatcttctt
     2581 agccctcacc cctctcccct cgcccctcgc ccctcacttg ttcctgtatt tatcgtggca
     2641 agccttgcag gccttgccgg tctctccgag ctgcgccttg ctggtattga gatcgccggc
     2701 cttcgcgact tcggccaact tggcaacctc ggcctgcatc ttctccagct tggccttgaa
     2761 gtcgtccata tcgagccaga tttccggctt ggccttggtt tcgccggtgt ccgatccggg
     2821 cacgaagcct tccagcggca gtttgctcat gaaggcgacg atctccgcgt ggcgcgcgaa
     2881 tgcggcggcg tcgtacggcc ggtcgccctt cgccatcgcc gccatcggac cgaagtgcca
     2941 gccgatcact gccattactc ctcttcgaaa cttgatcgcg tcctcgggct tctgcgcgaa
     3001 tgcggcattg gcgaacacgg atgcacagac agcgaacaac agcgcagcgg caaggcgttt
     3061 cattttttct cctcctgaaa taataaaaag ttggggagat tctaacacca gccccctctc
     3121 cccacccctc tcccgcgagg ggagagggag ttctgaatcg agaaccagtc ctgcagttgc
     3181 gtccgtcgtt gctgaatcga ttggcgccga taaaccctcc ggctccgatg aacaaggagc
     3241 ctgtctgtac agttgaattt actccctctc ccctcgcggg agaggggtgg ggagaggggg
//
LOCUS       DASQOP010000226         3315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2924898, whole genome shotgun sequence.
ACCESSION   DASQOP010000226 DASQOP010000000
VERSION     DASQOP010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2924898"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1749)
                     /locus_tag="VE030_09110"
     CDS             complement(<1..1749)
                     /locus_tag="VE030_09110"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HZF23610.1"
                     /translation="MTNLKPVDVAIIGGGWTGLLTAKEITEKTSLSVAVFERGGPRRL
                     ADYAVTMDELDYNIRLRMMQNTAEETITHRHSLKDSAVPVRQHGSFHPGTGTGGAGEH
                     WGGASFRFLPDVFTLRTHLLQKHGAARLPEDLSIQDWAVTYDDIEPRYWKAEQMMGIS
                     GKAGNLRGQKIDGGNIFEGPRRQEYPTPPLKHSYASSMFEAAVRRLGYHPYPTPAANL
                     SEPYKNPDGIMRGACQYCGHCQRYGCMVGAKAQPSNLLMPVLARRKNFEFRTGSWVRR
                     IVHKDGRATGIQFTGENGEEFFQPASTVVLASFTLNNSRLLLLSKIGTPYDPNTRKGT
                     LGRNLTHQVGTNTRVFFDKPLNAFMGAGELGSRISDFDGDIGFTGAEGEPLRLGMIAL
                     SMNGRPPIGSFGIMPQGASKSNWGSEWKKTALEWTDRSASIGLAGEHLAWRENYMDLD
                     PAYTDKFGDPLLRFTLNWTDHEYKQREFAEQIQARIAREMGAKFDEGPRPRARYNVIN
                     YQSTHIQGGAIMGTSPENSVVNRYLQHWDVPNLFVIGASAFPHNAAPNPTLTVLALTY
                     MASETMINRYFKHPETL"
     gene            2002..2766
                     /gene="modA"
                     /locus_tag="VE030_09115"
     CDS             2002..2766
                     /gene="modA"
                     /locus_tag="VE030_09115"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HZF23611.1"
                     /translation="MKCAGAIVGLVLALATHVEAAEIKVISTGGARAVMTSLVPEFER
                     KSGHKVSIDFGTPGNMRDRLLQGEAADVAVAIAAILPDLEKAGKIASGTRMEFAASYV
                     GVAVRAGAPKLDLSTPDGVKRAMLMAKTVALSDPKAGTQLGATFIGVADRLGFGADLR
                     SRTKLILGPGSDVAEAVARGEAELGVTLISEILPVTGVALGGELPSNIMPPTVIHAFL
                     VSGAKDTETAKVFLDFLRSPEASKIIEAKGMKPGPR"
     gene            complement(2810..>3315)
                     /locus_tag="VE030_09120"
     CDS             complement(2810..>3315)
                     /locus_tag="VE030_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23612.1"
                     /translation="DLNAGGTGKIAQPTGPGAISLYSPGASEPLRKLNNYLRRDGNIL
                     GKGITELAILVAAREGDSQYIWSAHEPAALKAGVAQPVIDAVKYGKDVPAAGEKETVV
                     VRLGRQILREHKLDSATFAKAVDLFGKQGTVELVILMGDYTLNAMLLDALDQHLPADR
                     KPLLPPR"
BASE COUNT          588 a          988 c         1044 g          695 t
ORIGIN      
        1 aagtgtctcc ggatgcttga agtatcggtt gatcatggtc tcggacgcca tgtaagtcag
       61 cgccagaacg gtcagagtcg gattcggcgc ggcattatgc ggaaatgcgg atgcgccgat
      121 cacgaacaga ttcggcacat cccaatgctg gagataacgg ttgaccacgc tgttttcggg
      181 tgaggttccc atgatggctc cgccctgaat gtgcgtggac tggtagttga tcacgttata
      241 acgggcgcgg ggccgtgggc cttcgtcgaa tttcgcgccc atctcacgcg cgatcctggc
      301 ctggatctgc tcggcgaact cgcgctgctt gtactcgtgg tcggtccagt tgagcgtgaa
      361 gcgcagcagc ggatcgccga acttgtcggt gtaagccgga tccaggtcca tgtaattctc
      421 gcgccaggcc agatgctctc ccgccagacc aatggaggcg gagcgatccg tccattcaag
      481 cgccgtcttc ttccactccg agccccagtt ggatttgctc gcgccctgcg gcatgatgcc
      541 gaaggacccg ataggaggac ggccgttcat gctgagggcg atcatgccga ggcgcaaggg
      601 ctcgccttcg gcgccggtga atccgatgtc gccgtcaaaa tccgagatgc gcgatcccag
      661 ttccccggct cccataaagg cgttgagcgg cttgtcgaaa aacacgcgcg tattggttcc
      721 cacctggtgc gtgaggttgc ggcccagcgt gcccttgcgc gtgtttggat cgtagggcgt
      781 accgattttg gaaagcagga gcaggcgcga attgttcagc gtgaacgacg ccagcacgac
      841 cgtcgaggcg ggttggaaga actcctcgcc gttctcgccg gtgaactgga ttccggtggc
      901 gcggccgtcc ttgtgcacga tgcgccgcac ccagcttccg gtgcggaatt cgaaattctt
      961 gcgccgcgcg agcactggca tcagaaggtt gctcggctgc gctttggcgc cgaccatgca
     1021 gccgtaccgc tggcaatggc cgcaatactg gcacgcgccg cgcataatgc cgtccggatt
     1081 cttgtacggt tcgctcaagt tcgccgcggg agtgggatag ggatgatagc ccagcctgcg
     1141 cacggccgct tcaaacatgc tgctcgcgta ggagtgcttg agcggcggcg tgggatactc
     1201 ctgccggcgc ggcccttcaa agatgttgcc accatcgatt ttctggccgc gcagattgcc
     1261 ggctttcccg ctgatgccca tcatctgctc ggccttccag tagcgcggct cgatatcgtc
     1321 gtaggtgacc gcccaatcct gaatcgagag gtcctcgggc agcctggccg ctccgtgttt
     1381 ctgaagcagg tgggtgcgca gcgtgaacac atccggcagg aagcggaagg aggcgccgcc
     1441 ccaatgctcg cccgcgccgc ccgtgcccgt gccgggatgg aaggacccat gctgccgcac
     1501 cggaacggcg ctgtccttca gcgaatgccg gtgggtgatg gtttcctccg ccgtattttg
     1561 catcatgcgc agacggatgt tgtagtcgag ctcatccatc gtcaccgcgt agtccgcgag
     1621 cctgcgcgga ccgccgcgct caaagacggc caccgacagg gacgttttct cggtgatttc
     1681 cttggccgtc agtagaccgg tccagccgcc gccgatgatg gcgacgtcaa cgggcttgag
     1741 attggtcatg agagagcctc gcttccttct ccgctatcaa ttgttcggag cagtccgaac
     1801 catactgcca cgtgcagtcg gcgttgtcga ggttcgctta gaccaggtcg cgactcgtca
     1861 catgcatggt cagccggctc gcggaaactt gacgattcgc gttgcgtcct cgtcattcag
     1921 agcattcgcc tggttgatct ctctcttcgg ctcgtatata agcccctttg gtgctctaca
     1981 tgtcctcata ggagagtagg tatgaaatgc gcaggcgcta tcgttggttt ggttttggct
     2041 ctggccaccc acgtcgaggc agcggaaata aaggtcatct ccaccggcgg cgcgcgggcg
     2101 gtaatgacaa gcctcgtccc tgaattcgaa cgcaagtcgg gccacaaggt tagcatcgac
     2161 ttcgggacgc cgggaaacat gcgggacagg ctgcttcagg gcgaagccgc tgatgttgcg
     2221 gttgcgatcg cagcaatcct gcccgatctt gaaaaggccg gcaagattgc atccggcacg
     2281 cgcatggaat ttgccgccag ttacgttggc gttgcggtgc gagcaggcgc gccaaagctt
     2341 gacctgagta cgcctgacgg cgtcaagcga gcgatgctca tggcaaagac ggtcgcgctg
     2401 tctgatccaa aggccggcac ccagctcgga gccacattta ttggcgtcgc agatcgcctt
     2461 ggtttcggcg ccgatcttcg atcccgcaca aaactgatcc ttggcccagg atcggatgtg
     2521 gcggaagcgg tggcgagagg ggaggcggaa ctcggcgtca ccctgataag tgaaatattg
     2581 ccggtgaccg gcgttgcact tggcggtgaa ctgccgtcaa acatcatgcc gcctaccgtc
     2641 attcatgctt ttttggtgtc gggggcaaag gataccgaga ccgcgaaggt tttcctggat
     2701 tttctccgct ctcccgaagc cagcaaaata atcgaagcta agggaatgaa gccgggtccg
     2761 cgctaggatt ggactgcccg cgcgtccatt ccggtcggcc gcgcgctgac tagcgcggcg
     2821 gcaggagcgg cttccggtcg gctggcagat gctggtccaa agcatcgagc agcatggcgt
     2881 tcagcgtgta gtctcccatc aggatgacca actccacagt gccctgtttt ccgaagagat
     2941 ccacggcttt cgcgaacgtg gcggaatcca gtttgtgctc gcgaagtatc tggcggccca
     3001 agcggacgac caccgtctct ttttctcccg cagcagggac gtccttgccg tatttgaccg
     3061 cgtcgataac cggctgcgcg acgcctgctt tcagtgcggc cggctcgtgc gcgctccaaa
     3121 tgtattgcga atctccttcg cgcgcggcca ccaggatggc cagctcggtg atgcccttgc
     3181 ccaggatgtt cccgtctcgc cgcaaatagt tgttcagctt gcgcagcggc tcggacgctc
     3241 cggggctgta cagcgagatg gcgccgggac ccgtcggttg ggcgatcttg ccggtgccgc
     3301 cggcgttaag gtcgt
//
LOCUS       DASQOP010000227         2551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_510033, whole genome shotgun sequence.
ACCESSION   DASQOP010000227 DASQOP010000000
VERSION     DASQOP010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510033"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /gene="lexA"
                     /locus_tag="VE030_09125"
     CDS             complement(<1..610)
                     /gene="lexA"
                     /locus_tag="VE030_09125"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HZF23613.1"
                     /translation="MSDLTPRQTQILRLIQTRIADSGMPPTRAEIARELGFKSANAAE
                     EHLRALQRKGVIALIPGASRGIQLKDTMREQLGLPLIGRVAAGRPILAEEHIEGRFQI
                     DPQLFQPRPHYLLKVSGMSMRDAGIFDGDLVAVHRTAEVRNRQIVVARLENEVTVKRY
                     RQEGSIVWLLPENADFEPIRVDLKAQAMIIEGIVVGILRRVDG"
     gene            1134..1451
                     /locus_tag="VE030_09130"
     CDS             1134..1451
                     /locus_tag="VE030_09130"
                     /inference="COORDINATES: protein motif:HMM:NF040598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine-zipper protein"
                     /protein_id="HZF23614.1"
                     /translation="MKSAMMLKASTAVLCLGLMAACTDLKPIQAQIDELKSQVAKVGA
                     DATSAKDAANSASSAASAAQSAANGAQSTANQALSAAQASQSCCDATNEKIDRMFKKS
                     MSK"
     gene            complement(1528..2496)
                     /locus_tag="VE030_09135"
     CDS             complement(1528..2496)
                     /locus_tag="VE030_09135"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HZF23615.1"
                     /translation="MDLIRSVAPWSAGLMLAVSGAVHAEMFQVPGAGVDIVGLGEKTV
                     AQFEDTLPDIARRYGLGFEEIALANPGTDPWLPGEGKDIRLPTQHILPPAPRDGIVIN
                     LADHRLYYFPRQTGNEPAELITHPISVGQMDWRTPLGITQIVAKIKKPTWYPPATIRK
                     QHEDDGDPIPAAIPPGPDNPLGDYAMRLGIPGGAYLIHGTNKPVGVGMQVTHGCIRMY
                     PEDIESLFRMVKVGTQVRIINQPVKTGWNGGVLYLEVHPPLENSGSTPIDLTELTRIL
                     VAATRDRPVRINWPLAERTLLEARGMPMAISLPQAGIEARASSESE"
BASE COUNT          471 a          785 c          800 g          495 t
ORIGIN      
        1 cgccatcgac ccgccgcaag attccgacca cgatgccctc gatgatcatg gcctgcgcct
       61 tcagatcgac cctgatcggt tcgaagtccg cgttctccgg cagcagccac acgatggatc
      121 cttcctgtcg atagcgcttg acggtgactt cgttttccag ccgcgcgacc acgatctggc
      181 ggttgcgaac ctcggcagtg cgatgtaccg caaccaggtc gccgtcgaag atcccggcat
      241 cgcgcatgct catgccgctc accttcagca ggtagtgcgg acgcggctgg aacaactgcg
      301 gatcgatctg aaagcggccc tcgatatgct cctcggcgag gatcggtcga cctgccgcca
      361 ccctgccgat caatggcagt cccagctgct cgcgcatcgt gtccttgagc tggataccac
      421 gagaagctcc cgggatcagt gcaatgacac ctttgcgctg cagtgcgcgc agatgctctt
      481 cggcggcatt cgccgacttg aagccgagct cgcgcgcaat ctcggcgcgc gtcgggggca
      541 tgccgctgtc ggcgatgcgt gtctggatca ggcgcaggat ctgcgtttgc cgtggcgtca
      601 ggtcggacat gagggaaacc gcaacgattt atccgggctg tgattatata cagttgcgcg
      661 caatgcgcaa attccagcat ccagacgcgg cgccgcatcc accctgcccg cgctgctgat
      721 caacttcggg agcgaggatg tgattgcgcc gatgaatgca cggacactcg ctgagggatg
      781 aaaggcacgc atgcgtacac gtcgatgcgc agtcggccaa cctcatcgtg gatcgatagc
      841 tcgtgttcga gcagtgcgaa catgagaact gaatcatctg cgcctgcatg gtgcagttga
      901 tgcgccgaaa ccggagacag tttcgcgcgc gctgcgttgt agaacttgcg cggcaagtca
      961 atagttgtcg ctgcaggacg acaagggcgc gagtactatt gcatccggcg cgcaaatctg
     1021 ttcatttatg cgccgttccg gttcggctcg cgcgtgggct tcggcccgtg gtttgagctg
     1081 aagtgttgga cttcgggcta cccgagaatc tcacgaacga aaaggggact accatgaaga
     1141 gtgcaatgat gctgaaggct tcaacggccg tactgtgtct cggtttgatg gctgcttgca
     1201 cagacctcaa gccgatccag gcgcagatcg acgagctgaa gtcacaagtg gccaaggttg
     1261 gcgcagatgc gacatcggcg aaggatgcgg cgaactccgc ttccagcgca gcttcggcgg
     1321 cgcagtcggc cgcgaacggt gcgcagagca ctgcgaacca ggcgttgtcc gcagcgcagg
     1381 cgagtcagtc gtgctgtgac gcgaccaacg agaagatcga ccgcatgttc aagaagtcga
     1441 tgtcgaagta atcggtctta cgatcaatct ggcgccgcgg agctcaaggg cccgcggcgc
     1501 ttttttttgc acggccctca gcctcgtcta ttccgattcg ctgctggcgc gcgcctcgat
     1561 cccggcctgc ggcagcgaaa tcgccatcgg catgccgcgc gcctccagca gagtgcgttc
     1621 ggccagcggc cagttgatac gcaccggccg atcgcgggtc gccgccacca ggatgcgcgt
     1681 cagctcggtc agatcgatcg gcgtcgagcc actgttctcc agcggcggat gcacctcgag
     1741 atacaacacg ccgccattcc agcccgtctt gaccggctga ttgatgatgc ggacctgcgt
     1801 accgactttc accatgcgga acagcgactc gatgtcctcg ggatacatgc gaatacagcc
     1861 gtgcgtcact tgcatgccga cgcccaccgg tttgttggtg ccgtggatca ggtacgcacc
     1921 gcctggaatg cccagccgca tcgcatagtc gccgagcggg ttgtcggggc ccggaggaat
     1981 ggcggctgga atcggatcgc cgtcgtcctc gtgctgcttg cgaatcgtgg ccggtgggta
     2041 ccaggtgggc ttcttgatct tggctacgat ctgcgtgatg ccgagcggcg tgcgccagtc
     2101 catctggccc acgctgatcg ggtgagtgat gagctctgcc ggctcattgc cggtctggcg
     2161 cgggaaatag tagagacgat ggtccgcgag gttgatgacg atgccatcgc ggggcgccgg
     2221 cggcaggatg tgctgcgtgg gcagcctgat gtccttgccc tcacccggca accagggatc
     2281 cgtgcccggg ttcgccaacg cgatctcctc gaaaccgagg ccgtagcggc gagcgatgtc
     2341 tggcagcgta tcttcgaact gcgccaccgt tttctctccg agcccgacga tgtccacccc
     2401 ggctccaggt acctggaaca tctctgcgtg caccgcacca gaaacggcga gcatcaatcc
     2461 cgcgctccac ggcgcaactg agcggataag gtccatgaga cgcattctag gccagtattg
     2521 tgatgagtct cacgtccggc gaggccaccg t
//
LOCUS       DASQOP010000228         8560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_305475, whole genome shotgun sequence.
ACCESSION   DASQOP010000228 DASQOP010000000
VERSION     DASQOP010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8560
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305475"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..1641)
                     /locus_tag="VE030_09140"
     CDS             complement(115..1641)
                     /locus_tag="VE030_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZF23616.1"
                     /translation="MGANSTEFITGGREVDLPARPFRGRMLTDGAWIEAADCERIDRE
                     SPAHGVAVSSYPAAKTADVDRAVSAARKAFDRGAWPRLKGAQREAILRRVAEGIVRRK
                     EELALLETLESGKPISQARDEMSGCTDLWVYAAALARNLHGDSYNTLGEDMLGLVIKE
                     PIGVVSLITPWNFPLWILSQKLPFALAAGCTAVVKPSEFTSATTLILGEILLEAGVPA
                     GVVNIVTGTGPDCGAPMVSHPQVDMISFTGSTRVGKAAAATAAGALKKVALELGGKNP
                     QIVFPDCDWEAALDAVVFGVYFNAGECCNSGSRLLVHADIAERFTEAVIERARRVPVG
                     EPLNTRTKVGAIINRNQMRIIGKYLSEATSAGAQVRLGGEPISGVVGLFMQPTVLTGV
                     TREMRVAREEIFGPVLSVLTFETAEEAVAIANETLYGLSAAVWSRDFDICLTAARRIK
                     AGTIWINSFMDGYPELPFGGYRESGIGRELGRAAAEDYTETKTVQMHVGARTGWWLPR
                     "
     gene            complement(1643..2431)
                     /locus_tag="VE030_09145"
     CDS             complement(1643..2431)
                     /locus_tag="VE030_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZF23617.1"
                     /translation="MPDAGASQVRLQSDGAVAIVTLDRAEKLNALTPEMIDALAEAAS
                     RIDRDAGIRCAILTATGDKAFCVGADVNAWSGLEPLDMWRRWVKRGHQVFDQWARLRV
                     PVIAAINGHALGGGLELAAVADIRITVSAATFGLPEATIATCPGWSGTQRLAALIGSS
                     RVKYLALTGMRIDATEAWRVGIVHERVEGKDVLARAREIASGICKLAPVSVQLTKQVI
                     DAGQGEGLAAALEAMAGALAATTADAREGVASFLDKRQPDYRGK"
     gene            complement(2424..4013)
                     /locus_tag="VE030_09150"
     CDS             complement(2424..4013)
                     /locus_tag="VE030_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HZF23618.1"
                     /translation="MNKVISAEQAAQLVRDGDVVTVSSSSGLGCPDKTLAALGSRFAQ
                     QGHPHSLTTLHPIAAGDMYGIRGIDHLARPGMLKKVLAGSYPSGPSSLPMPDIWRLIV
                     EEKIAAYNLPSGVLFDMHRDAAARRPGVLTKVGLDTFVDPDRQGGAMNARAQSEPFVK
                     KVSFAGEEWLFYPAIVPRVAILRATTADERGNLSYEHEGAFLGSLDQALAARNNGGVV
                     IAQVKRVVRAGSLNPQLVHVPCTLVDHVVVDPEQMQTTQTAYDPAISGEIFRPDDSFE
                     LPAWGPEKVIARRAAQELAAGSAANLGFGISANVPRVLIEEGLHGEVTWVIEQGAVGG
                     VPLLGFAFGCAANADAIIPSPNQFTYFQGGGFDVALLSFLQIDRAGNVNVSKLGAKPY
                     LTAGCGGFVDITTHAAHIVFSGFFTAGAKLEVGHGQLKIRQEGKSQKLVDAVEHVTFS
                     GTVARRRQQKIRYVTERCVLDLLEDGLVVREIAPGVDLRRDVLDQAAFPLRVDAQLKT
                     MDAALFRDAPMGLRLKGRAGA"
     gene            complement(4031..5044)
                     /locus_tag="VE030_09155"
     CDS             complement(4031..5044)
                     /locus_tag="VE030_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZF23619.1"
                     /translation="MAKQEIGWGLVGASTIAREWAIDAIRAQAGNDVVAVMSADPARA
                     RDYAQANRIPVSYTSLAALLADPNVDAVYISTTNELHKAQTLACARAGKHVLCEKPLA
                     LTLADAREMVAACARAGVVMGTNHHLRNAATHRKIRELIQSGAIGTPLFARVFHAVYL
                     PPHLQGWRLSNASAGAGVILDITVHDADTLRFVLGAEPVEAIAVSQQAGMAKGGLEDG
                     VMAVLRFDNNVLAQLHDAFTVKFAGTGVEVHGTEGSIIARDVMTQRPIGQVALRTSQG
                     ETNVPVVHENLYARGLHAFVAAVRGEGAPAASGEDGVRSLATALAVLEASRSGRRVEI
                     KYQ"
     gene            5213..6253
                     /locus_tag="VE030_09160"
     CDS             5213..6253
                     /locus_tag="VE030_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZF23620.1"
                     /translation="MTQPQGNAKRASVTITDVAHAAGVSKSTVSLVLQGSPLIARETA
                     ARVRQSAIALGYVYNRRAADLRRKSSNMVGIVINDLGNPFFAEMLVGMEHRLVDAGYI
                     SLMAHTDERLDVQERVLTSMQEYHAAGLILCPAFDTPPGLLQRTQHSGIPLVIVVREP
                     GTGSYDFVGANHESGTFEVTRHLIGQGHRRIAFLGRIGGGPVYEQRRTGYQRAMTENG
                     IAVVPEWIVDIPPTREGGREGVRQVLAMPVRPTAAVCYNDVVAFGTLGELGEHGLVVG
                     RDFAITGFDGVAATAHSNPPLTTIDMRPGEQGAVAADMLLKRLADPAAAPLRYILEPR
                     LVVRQSSGPPPA"
     gene            6295..6771
                     /locus_tag="VE030_09165"
     CDS             6295..6771
                     /locus_tag="VE030_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-degrading domain-containing protein"
                     /protein_id="HZF23621.1"
                     /translation="MDNQQQLQLLLQQESELQFAEFTNDTAVALGLKLVEAARARNCS
                     VTVDVCRNGQQLFHCALPGTSADNDDWIKRKNNVVNRYGHSSLYVGTQFRARGAKFEE
                     SSRLDPGHYSAHGGAFPVTIRNVGVVGTVTVSGLPQVEDHELVVEVLRDFLGSGRH"
     gene            complement(6790..7287)
                     /locus_tag="VE030_09170"
     CDS             complement(6790..7287)
                     /locus_tag="VE030_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23622.1"
                     /translation="MEQRKDGFLSARFAAEQFAQMNREAAVVVAEDQRRIVSYACSAG
                     VNFSRQFPILDAMIAAFGRLTYLGMTLIDARVCIYGPVCVDRAWRGRGVLRGVVARMK
                     EELAGQFDVAAAFISKANSRSLAAHVEGLGMTVLGDYAFGTGRYWIVAFSIPPADSAC
                     GVGPR"
     gene            complement(7514..7912)
                     /locus_tag="VE030_09175"
     CDS             complement(7514..7912)
                     /locus_tag="VE030_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HZF23623.1"
                     /translation="MPFDPTSQRGNRPRNVLGGPLADCCNNPVTGFFRDGCCNTGPED
                     HGAHVVCVQVTKEFLEFSVARGNDLSTPMPDFGFPGLNPGDRWCLCAARWREALSAGM
                     APSVVLTATHERALDYVTLEALKKYALDLQ"
     gene            complement(7921..>8560)
                     /locus_tag="VE030_09180"
     CDS             complement(7921..>8560)
                     /locus_tag="VE030_09180"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HZF23624.1"
                     /translation="RRIELRLSADGTMTWLTDYRNNRPPITEEGRWNAISVEEIEVIV
                     ERRGGKQVEPFAVRLLKQGDTLRATGVSAAEFGSHGLLLKLAKAAVPPIAAPATAALS
                     PIGVWQWERLISSAEKVAVDRPERYKLQLQAGGKAQVLADCNRGQASYSFDGRSFSIK
                     ITGITRAACPAGSLSGRYLRVLETAVGQRVKGENLFLDLPADGGTMKFVRAK"
BASE COUNT         1541 a         2737 c         2680 g         1602 t
ORIGIN      
        1 aaccgtggca atcaatttcc tacgcatgtt ctcctcctgt tatcagtatt gaatgcccga
       61 ttacggccgg acaattgccg atctgcaaac tgcaaatttc ttgagtgtgc caaattatcg
      121 tggcaaccac catccggtac gcgctccgac atgcatctgg actgtcttgg tctcggtgta
      181 atcttccgcc gccgcccggc cgagttcgcg accgattccg ctttcgcggt agccgccgaa
      241 cggcaactcc ggatagccgt ccataaagct gtttatccat atcgtacccg cctttatgcg
      301 acgggccgca gtcaggcaga tgtcgaagtc ccggctccaa acagccgccg acagcccgta
      361 cagggtttcg ttggcgatcg cgaccgcttc ctccgcggtc tcgaaggtca gcaccgacag
      421 cactgggccg aagatctcct cccgggccac gcgcatttcc cgggtcaccc cggtgagcac
      481 ggtaggctgc atgaacaacc ccaccacgcc ggaaatcggt tcacctccca ggcgcacctg
      541 cgcacccgcg gatgttgcct ccgacagata cttgccgatg atacgcatct ggttgcggtt
      601 gatgatggcg ccgaccttgg tcctggtgtt cagcggctcg cccacgggga cgcgccgggc
      661 gcgctcgatg acggcctcgg taaaccgctc cgcgatatct gcatgaacca gcagccggct
      721 gccgctgttg cagcattcgc cagcgttgaa gtacacgccg aaaactaccg cgtcgagcgc
      781 agcctcccaa tcgcaatccg ggaacacgat ctgcgggttc ttgccgccca gttccagagc
      841 gacctttttg agcgcgccgg ccgcggtcgc ggcagcggcc ttgccgacgc gggtcgagcc
      901 agtgaatgag atcatgtcca cctgcgggtg gctgaccatg ggggcgccgc agtcgggacc
      961 ggtgccggtg acgatgttca ccacccccgc cggtactccg gcctcgagca agatctcgcc
     1021 caggatcagc gtcgtggcgg aggtgaattc gctcggtttg accacggccg tgcaaccggc
     1081 ggcaagcgcg aatggcagct tctggctcag aatccagagc ggaaaattcc acggcgtgat
     1141 caggctcacc acgccgatcg gttctttgat caccagcccg agcatgtctt cgccgagcgt
     1201 gttgtagctg tcgccgtgca ggttgcgggc aagtgccgcg gcatagaccc agagatcggt
     1261 gcagccggac atctcgtcac gcgcctggct gatcggtttg cccgattcca gcgtttcgag
     1321 caaggcgagt tcctccttgc ggcgcacgat tccttcggca acccggcgca gaatcgcctc
     1381 tcgttgcgca cccttgagac gcggccacgc gccacgatcg aaggctttgc gcgccgcgga
     1441 gaccgcgcga tccacgtcgg ccgtcttcgc tgccgggtaa ctggaaacgg cgacgccgtg
     1501 ggccgggctt tcgcggtcga tgcgttcgca gtccgcagcc tctatccagg ccccgtcagt
     1561 cagcatgcgg ccgcgaaacg gccgcgcagg cagatcgacc tcgcggccgc cggtgatgaa
     1621 ttccgtcgaa tttgcaccca ctttattttc ctctgtagtc gggctgccgc ttgtcgagaa
     1681 agctggccac accctcgcga gcgtctgctg tcgtggcagc cagcgcgccc gccattgcct
     1741 ccagtgcggc cgccagacct tcgccttggc cggcgtcgat cacctgcttg gtgagttgca
     1801 ccgagaccgg cgccagcttg catatgccag aggcgatctc ccgcgcccgc gccagcacgt
     1861 ctttgccttc gacgcgttcg tgcacgatac ctacgcgcca ggcttcggtg gcgtcgatac
     1921 gcatgccggt cagagcgaga tacttgaccc ggctgctgcc gatcagcgcg gcgagccgct
     1981 gcgttcccga ccaaccgggg caggtcgcga tcgtggcctc cggcagtccg aacgtcgccg
     2041 ctgaaacggt gattcggatg tcggcgaccg cggcgagctc gaggccgcca cccagtgcgt
     2101 ggccattaat cgcggcgatc acgggcacgc gtaaccgcgc ccactggtcg aatacctgat
     2161 gcccgcgctt tacccaacgg cgccacatgt cgagcggctc cagtcccgac caggcattga
     2221 catcggcgcc gacgcagaag gccttgtcgc ccgtggctgt caggatcgcg cagcggatcc
     2281 cggcatcgcg atcgatacgg ctggcggctt ccgccagcgc gtcgatcatc tccggcgtca
     2341 gcgcattgag cttttccgcg cggtcgagcg tgacgatcgc cactgcgccg tcgctttgca
     2401 gccgtacctg gcttgccccg gcgtcaggca ccggcccgcc ctttgagcct taggcccatc
     2461 ggcgcatcgc ggaacagcgc cgcatccatg gttttcagtt gcgcatcgac ccgcagcggg
     2521 aacgccgcct gatccagcac gtcgcgccgc aagtccacgc cgggcgcaat ctcccgcacc
     2581 acgagtccgt cttcgagcag atcgagcacg cagcgttcgg tgacgtagcg gattttctgc
     2641 tgccggcgcc gcgcgacggt gccgctgaac gtgacatgct cgaccgcgtc caccagtttc
     2701 tggctcttgc cttcctgccg gatcttgagc tggccgtggc cgacctcgag cttcgcgccc
     2761 gcggtgaaaa agccggaaaa gacaatgtgc gcggcgtggg tggtgatgtc gacgaaaccg
     2821 ccgcagccgg cagtaaggta aggcttggcc ccgagtttcg agacgtttac gttgccggcg
     2881 cggtcgatct gcagaaacga cagcagcgca acgtcaaatc cgccgccctg aaaataggtg
     2941 aactgattgg gagaagggat gatcgcgtcg gcgtttgccg cgcagccaaa ggcgaagccg
     3001 agcaacggta cgccgccgac cgcgccctgc tcgattaccc aggtgacttc gccgtgcagg
     3061 ccttcctcga tcagcacgcg cggcacgttg gcggagatgc cgaagccgag gttggcggcc
     3121 gagccagccg ccagctcctg cgcggcacgc cgcgcgataa ctttctccgg cccccatgcc
     3181 ggcagttcga aggaatcgtc agggcgaaat atttcgccgc tgattgccgg atcgtaagcc
     3241 gtctgcgtgg tctgcatctg ttccggatcg accacaacgt ggtctaccag tgtgcagggc
     3301 acgtgcacta gctgcggatt gagcgagccg gcgcgtacca cgcgcttgac ctgggcgata
     3361 accacgccgc cgttgttgcg ggccgcgagc gcctggtcca gcgagccgag gaaagcgcct
     3421 tcgtgctcgt aagagagatt gccgcgctca tccgctgtgg tggcgcgcag gatagccacc
     3481 cgcggcacga tcgccggata gaacaaccat tcttccccgg cgaacgacac tttcttcacg
     3541 aagggctcgg attgcgcgcg cgcgttcatg gcgccgcctt ggcggtccgg atcgacgaat
     3601 gtgtccagcc cgaccttggt aagcaccccg gggcgcctgg cggcggcgtc acggtgcatg
     3661 tcgaacagca cgccgcttgg cagattgtag gccgcgattt tttcttcgac gatcaggcgc
     3721 cagatatcgg gcatcggcaa cgaagacggg ccgctcgggt aggagccggc cagcactttc
     3781 ttcagcatcc ccgggcgggc taggtggtcg atgccgcgga tcccatacat gtcgcccgcg
     3841 gcgatcggat gcagtgtcgt gaggctgtgc ggatggcctt gctgggcaaa acggctccca
     3901 agcgcggcga gcgtcttgtc agggcagccg agcccgctcg aagagctgac agtgacgacg
     3961 tcgccgtcgc gaaccagttg cgcagcctgc tcggcggaaa tgactttgtt catgcgctgc
     4021 ttcgaagggt ttactggtat ttgatttcga cgcggcgacc gctacgggac gcctccagca
     4081 ccgccagggc ggtggcgagt gaccggacgc cgtcttctcc gctggcagcg ggcgcgcctt
     4141 ctccgcgcac ggcggcaacg aacgcgtgca gaccccgggc gtaaagattt tcgtgcacga
     4201 ccgggacatt cgtctcgccc tgggacgtgc gcagcgcgac ctggccgatc gggcgttggg
     4261 tcatgacgtc gcgggcgatg atcgaaccct cggtgccatg tacttcgacc ccggtgccgg
     4321 caaacttcac ggtgaaagcg tcgtgaagtt gcgccagcac gttgttgtcg aagcgcaaca
     4381 ccgccatcac cccgtcttcg agcccccctt ttgccatgcc tgcctgttgg gagacggcga
     4441 ttgcctctac cggctcggca ccgaggacga agcgcagcgt gtcagcgtcg tgcacggtaa
     4501 tgtcgaggat gaccccggca cccgcagacg cattgctgag ccgccagccc tgcaggtggg
     4561 gcggcaaata gacggcgtgg aaaacccggg cgaaaagcgg agtgccgatg gcgccggatt
     4621 gaatcagctc ccgaatcttg cgatgcgtgg cggcgttgcg caggtgatgg ttggtgccca
     4681 tgaccacccc ggctctggcg caggcagcga ccatttccct ggcatcggca agcgtgagcg
     4741 ccaggggctt ttcgcacagc acgtgcttcc cggctctagc gcaggccagg gtctgtgctt
     4801 tgtgcaattc gtttgtggtg ctgatgtaga ccgcatccac attcgggtcc gcgagcaggg
     4861 cggccaacga cgtgtagctc acggggatcc ggttcgcttg ggcgtagtcc cgggcgcgag
     4921 ccggatcggc actcatcacc gcgacaacgt cgttgccggc ttgggcgcga atcgcatcga
     4981 ttgcccattc cctggcgatg gtgctggcac ccactaatcc ccagccaatt tcctgctttg
     5041 ccatgggctc ccccattttt ccggagattc tcgtaaaagc cgagagttat cgtattttgt
     5101 atgctttata tcgttccaat aaagaacttg gaacgatcca attcagatat aatatggcgt
     5161 cccacgagaa tattgtcaat aatatttgcg gaatccttag ttggcagaat tcatgaccca
     5221 gccccaaggc aacgccaagc gcgcgtcggt caccattact gatgtcgcac acgcagcggg
     5281 tgtttccaag tcgaccgttt ccctggtttt acaaggcagc cccttgattg cccgcgagac
     5341 cgccgcgcgg gtgcgacaga gcgccatcgc gctcggttat gtgtacaacc ggcgggcagc
     5401 cgacctgcga cgcaagtcgt cgaacatggt tggcatcgtc atcaacgatc tcggcaaccc
     5461 gttctttgcc gagatgttgg tcggtatgga gcaccgcctg gtcgatgccg gttacatcag
     5521 cctcatggcc cataccgacg agcggctgga tgttcaggag agagtgctta cttcgatgca
     5581 ggagtaccac gccgcgggac tcatactatg tccggcattc gatacgccgc cagggctgct
     5641 gcagcggacc cagcattccg gcattccgct ggtgatcgtg gtacgcgaac cgggtaccgg
     5701 cagctacgac ttcgtcggcg ctaatcacga gagcggcact ttcgaggtga ctcgccacct
     5761 gatcgggcag gggcatcgcc ggattgcgtt cctgggccga atcggcggcg gaccggtcta
     5821 cgagcagcgg cgtaccggat atcagcgggc gatgacggaa aacggcatcg cggttgtgcc
     5881 cgaatggatc gtcgacattc ctcccacccg cgaaggcggc cgcgaaggcg tccggcaggt
     5941 gctggcgatg ccggtccgac cgacagcagc agtctgctac aacgacgtag tggcgttcgg
     6001 caccctgggc gaactgggcg agcacgggtt ggtggtcggc cgggatttcg cgatcaccgg
     6061 attcgacggc gtcgcggcga ccgcgcattc caacccaccg ctcaccacca tcgacatgcg
     6121 gccgggcgag caaggcgccg tggcggccga catgctgctc aaacggctcg ccgatccggc
     6181 ggcggcaccg ctgcgatata ttctcgaacc gcgactggtg gtgcgccagt cgtccggacc
     6241 gccgccggcg taaaggggcg catcagaaat tattgcaagc cggacaccgg tcgaatggat
     6301 aaccaacaac agctacagtt gctcctgcaa caagaatccg agttgcagtt cgcggagttc
     6361 accaacgaca ccgcggtggc actcgggctg aagctcgtcg aagcggcccg ggcacgcaac
     6421 tgttcagtca ccgtcgacgt gtgccgtaac ggccaacagt tgtttcactg cgcgcttcca
     6481 ggtacgtctg cggacaacga cgactggatc aagcgcaaga acaacgtagt caatcgctat
     6541 gggcacagct cgctttacgt cggcacgcag ttccgcgcca ggggcgccaa gttcgaggag
     6601 tcgtcgcggc tggatcccgg ccattattca gcgcacggcg gcgcgttccc ggtcactatc
     6661 cgaaacgtcg gcgtggtggg aactgtgacc gtctcgggct tgccgcaggt tgaagaccac
     6721 gaactggttg tcgaagtatt gcgcgatttt ctgggcagcg gtcggcacta aagacgctgt
     6781 ctatcggtat taccgaggtc caactccgca agcggaatcg gccggcggga tgctaaaggc
     6841 gacgatccag tagcgaccgg tcccgaatgc gtagtcgccg aggacggtca tccccaggcc
     6901 ttccacatgc gcggcgagcg agcgcgaatt ggccttggaa atgaaagccg ccgccacatc
     6961 gaactgcccg gccagttcct ccttcatcct ggcaaccact ccgcgcagca cgccgcgtcc
     7021 gcgccaggcg cgatcaacgc acaccggccc gtaaatgcat acgcgcgcat cgatcaaggt
     7081 catgcccagg taggtgagcc gcccgaaggc cgcgatcatg gcgtcgagta tcgggaattg
     7141 gcggctgaaa ttcacacctg ccgagcaggc gtaactgacg atgcggcgct ggtcttccgc
     7201 aaccactact gccgcctcgc ggttcatctg cgcaaattgc tccgcggcaa agcgcgccga
     7261 caggaatcca tccttacgct gttccatggg cagattttcg aaaagattgg tttcctgcag
     7321 ttcgagaagg ctggggaggt cgcgggggtg gagacggcgg aaaatcattc cgaaaagcag
     7381 ggcagagata cgagggccgg ggtccgagag gggaggattc agtgagaaag aagtcggatg
     7441 cttctcgatg tatttgcggc tttcgttgga ttgcgtttat ccgcccctgg tacttcggcc
     7501 ctcgaacctg ttgttattgc aagtcgagcg cgtatttctt gagggcttcg agggtcacgt
     7561 agtcgagcgc ccgctcgtgg gttgctgtaa ggaccactga cggcgccatt ccggcactca
     7621 gcgcttcacg ccagcgcgcg gcgcacaggc accagcggtc gccgggattc agccccggga
     7681 agccaaagtc aggcataggc gtgctgaggt cgttaccgcg tgcgaccgag aattccagga
     7741 attcctttgt gacttgaacg catacgacat gcgccccgtg atcttccggc ccggtgttgc
     7801 agcagccgtc gcggaagaaa ccggtcaccg gattgttgca gcaatcggcc agcggcccgc
     7861 cgagtacgtt acgggggcga ttacctcgct gggaggttgg atcgaagggc atggtcctcg
     7921 ttatttcgcc cgtacgaatt tcatagtgcc gccatcggcc ggcagatcga gaaacaggtt
     7981 ttcgcccttg acgcgctgcc cgactgccgt ttccagcacc cttagatagc ggccggagag
     8041 cgagcccgcc ggacaggcag cgcgggtaat tccggtgatc ttgatgctga agctgcgacc
     8101 gtcgaatgaa taactggcct ggccgcggtt gcaatccgca agcacctgcg ccttgccgcc
     8161 cgcctgtaat tgcagcttgt agcgctccgg ccgatcgacc gcaactttct cggcggagga
     8221 gatcagtctt tcccattgcc agaccccgat gggagaaagc gctgcggttg ccggcgcggc
     8281 aatcggcggc actgctgctt tggcaagctt caacagcagg ccgtggctgc cgaattcagc
     8341 cgccgatacc cccgtggcac gcagcgtatc cccctgcttc agcaggcgaa cagcgaaagg
     8401 ttcgacctgc ttgccgccgc ggcgctcgac gatgacctcg atctcttcga ccgagatggc
     8461 gttccagcgg ccttcctcgg taatcggcgg acgattgttg cggtaatcgg tgagccaggt
     8521 cattgttcca tcggccgaga gccgcagctc gatccttcga
//
LOCUS       DASQOP010000229         8677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2121091, whole genome shotgun sequence.
ACCESSION   DASQOP010000229 DASQOP010000000
VERSION     DASQOP010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8677
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2121091"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..794
                     /gene="gpmA"
                     /locus_tag="VE030_09185"
     CDS             45..794
                     /gene="gpmA"
                     /locus_tag="VE030_09185"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HZF23625.1"
                     /translation="MHKIVLLRHGESVWNRENRFTGWTDVDLSDQGVVEAREAGRLLK
                     QAGFGFDLALGSVLKRAIRTLWIVLDELDLMWIPQQLSWRLNERHYGALQGLNKAEMA
                     AQYGADQILLWRRSYDVAPPPLARSDPRYPGNDPRYRDMKQSDIPLSECLKDTVARFL
                     PYWNDVIAPQVQAGKRVIVVAHGNTIRALVKYLDNVPEKDIVALNIPTGIPLVYELDD
                     HLKPLSSYYLGDQEKARQAAAAVAAQARAGT"
     gene            806..1993
                     /locus_tag="VE030_09190"
     CDS             806..1993
                     /locus_tag="VE030_09190"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HZF23626.1"
                     /translation="MRRSAFIAFLAVEAGAALLFALAAPAHAAPKDELEELRGRIGAL
                     QKQLAESEESKAEAGDALRESERAVSETNRKLYQLADQRHHVNAALAKLRSRARTVES
                     DITGEQATLSKLLYQQYVTGQSDAIKLVLNREDPNEIARQLHYLGYVSRARAELLANL
                     RYNLANLEKLADETRAKSAELRALQNEEASQKRRLEKEKVERQRVYAQVSSEIAQSRK
                     QLSTLKRDEERLTRLIERLAKEFTRKKPGKRIANQALPSADAGSGPFRKLKGKLRLPV
                     LGELMNRFGSPRQDSGLSWKGLFISAEPGREVKAIAGGRVVYADWLRGFGNLMIVDHG
                     DGYMSLYGNNEALLKQVGDETRPGDTLAAIGNSGGNPDSGLYFELRYQGKPFDPLPWV
                     ILK"
     gene            2040..3443
                     /locus_tag="VE030_09195"
     CDS             2040..3443
                     /locus_tag="VE030_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HZF23627.1"
                     /translation="MHSRFRQVGLIVLGAILGVLISLNFSAVAQKGAGPLPIEDLRAF
                     TEVFGRIKNDYVEPVDDKRLISEAINGMLSGLDPHSAFLDAEAFRELQVGTQGEFGGL
                     GIEVGMEDGFVKVVAPIEDTPAWRAGLKSGDLIIKLDDTNVKGMTLNDAVKRMRGKPD
                     SQITLTFIRKGDTKPRVVTLTRAIIQIQSVKSKLLEPGYAYFRVTQFQEHSAETLAKA
                     IDKHFKENQGPMKGIILDLRNDPGGLLNGAVAISAAFLPSDALVVYTEGRTEDAKMKL
                     YARPEYYLRGSKDDYLKKLPKEVKTLPMVVLVNSGSASASEIVAGALQDHKRAVVMGT
                     QTFGKGSVQTILPLGNGTAIKLTTARYFTPNGRSIQAKGITPDIVVEEATLTEADAAL
                     KVRESDLQRHLSNPDGSAIENKQPAPQPQSAVPVPAPKAGEKAAAESPPDIVSKSDYQ
                     LNQALNLLKGLQILSKK"
     gene            3534..4283
                     /gene="moeB"
                     /locus_tag="VE030_09200"
     CDS             3534..4283
                     /gene="moeB"
                     /locus_tag="VE030_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HZF23628.1"
                     /translation="MDDDQLLRYSRHILLPEIGIDGQEKLLGAHALLIGAGGLGAPAS
                     LYLAASGVGRITLCDHDAVDLTNLQRQIVHFTESIGRKKVDSARATLARINPEISIEA
                     IAERVAGLRLEQLVQSADVVIDATDNFATRHAVNRACVKFARPLVSGAGVRFDGQIAV
                     FDLRRRDSPCYHCLFPENGDVEEMRCAVMGVFAPLVGVIGSMQAAEALKLLMGIGRDL
                     TGRLLLLDALAMEWRSVRLKKDSQCAVCGGG"
     gene            complement(4423..4935)
                     /locus_tag="VE030_09205"
     CDS             complement(4423..4935)
                     /locus_tag="VE030_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23629.1"
                     /translation="MAKHCLMDGCARLNRTARTIAAVFFLMCAGVALSGCGASDDRFA
                     KVLVGTWGSREVAPDDVIVETQFTLLPGGRVNWQGQVRLLVPPDFYLPNRPNFEVDNG
                     RLIYYFTASGNWTVREGYLHTRIEASTLPSLMPVGFAAAWKLREVTSKEMIYVSAANG
                     RTRVEYRKSD"
     gene            complement(4917..5489)
                     /gene="slmA"
                     /locus_tag="VE030_09210"
     CDS             complement(4917..5489)
                     /gene="slmA"
                     /locus_tag="VE030_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HZF23630.1"
                     /translation="MPTKPGERKLQILQTLAAMLEDPKAEKITTAGLAKRLDVSEAAL
                     YRHFASKAQMFEGLIEFIEQTLFGLVNKITAEEKSGLKQIEAVLTTLLGFAKKNPGMT
                     RVLIGDALVNEDERLQARINQLHDRLEATLKQALRFALSEKQIPEGVDPTAQANLMLA
                     FVVGRWHQFAKSGFRRDPTEYWSAQWPSIV"
     gene            complement(5815..6465)
                     /locus_tag="VE030_09215"
     CDS             complement(5815..6465)
                     /locus_tag="VE030_09215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HZF23631.1"
                     /translation="MQSRFSGRTWIFDLDNTLHDARPHLFPHIDRSMTAYLAEHLDLS
                     EYAANELRQFYWRRYGATLVGLIRHHGIDPHHFLRETHRFPQIGDLVVGRRELRSVLR
                     RLPGHKIVFSNAPAHYSRAILAALGVDDLFDDVFSIERTRFRPKPDAHGFRRLLRTHR
                     LLASRCIMVEDSLENLRTAKRLGMKTVWVDRSSRSPAWVDANVRDIVRLPRLLHLL"
     gene            complement(6471..7358)
                     /gene="argB"
                     /locus_tag="VE030_09220"
     CDS             complement(6471..7358)
                     /gene="argB"
                     /locus_tag="VE030_09220"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HZF23632.1"
                     /translation="MPLSPSDATHKAQILAEALPYIRRFHGKTIVIKYGGNAMTDEKL
                     KHSFARDVVLLKLVGMDPVVVHGGGPQIDDFLKRVGKKGEFIQGMRVTDRETMDVVEM
                     VLGGLVNKDIVNLINQHGGRAIGLTGKDGSFIRARKMLVQNEDNAAEMLDIGQVGEIE
                     SIDPEVISLLESREFIPVVAPIGVGEDGQSYNINADLVAGKLAEILRAEKLILLTNTA
                     GVLDKNGNLLTGLTPKQVDGLFADGTIHGGMLPKIASALDAAKSGVKSVHIIDGRVEH
                     ALLLEVLTDQGVGTLIRNR"
     gene            complement(7463..7888)
                     /locus_tag="VE030_09225"
     CDS             complement(7463..7888)
                     /locus_tag="VE030_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HZF23633.1"
                     /translation="MASLARYSYPLDETFDDLFKGFFVRPMTFENQVQGQFRVDVKED
                     DGAYVVQAEIPGVKKEDINVTINGNQVAISAEVKRERQDKQGEKVLRSERYYGKAYRA
                     FSLEQDVDEATAQAKYNDGVLELRLPKKAAVTAKKLSIQ"
     gene            8033..8452
                     /locus_tag="VE030_09230"
     CDS             8033..8452
                     /locus_tag="VE030_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23634.1"
                     /translation="MRLRRPDGLDCIQAPWRGVPVNAVRAAASVRAEPGLPAGGLGNR
                     GNSSITPQGEPLMEGKKLAGMALAATAAALFMTGCANMGGGSGTSAKVHCEGANACKG
                     MSDCKSANSSCHGQNACKGQGFVSLTPAECRKVSGRA"
     gene            8526..>8677
                     /locus_tag="VE030_09235"
     CDS             8526..>8677
                     /locus_tag="VE030_09235"
                     /inference="COORDINATES: protein motif:HMM:NF016970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HZF23635.1"
                     /translation="MTAQPALGFGLGLRTDHYEDILASPPNVDWFEALTENYLVPGGK
                     PLHYLDR"
BASE COUNT         1681 a         2748 c         2689 g         1559 t
ORIGIN      
        1 aaatgctttt tcgcactgcg aaacgcactt cggcaaaaac aaccatgcac aagatcgttc
       61 tgttgcggca cggcgagtcg gtctggaaca gggaaaaccg ctttaccggc tggaccgatg
      121 tggacctgtc ggaccagggc gtggtcgagg cgcgcgaggc cggccggctg ctcaagcagg
      181 ccggattcgg gttcgacctc gcactaggct ccgtgctcaa gcgtgccatc cgcaccctgt
      241 ggattgtcct cgacgagctg gacctgatgt ggatcccgca gcagctttcc tggcggctca
      301 atgagcgtca ctacggcgcg ctgcaggggc tgaacaaggc cgagatggcg gcgcagtacg
      361 gcgcagacca gatcctgctg tggcgccgca gctacgacgt cgcgccgccg cctcttgcgc
      421 ggagcgatcc gcgctacccg ggcaacgacc cgcgctatcg cgacatgaag caaagcgaca
      481 ttccgctcag cgaatgtctc aaggacacgg tggcgcgttt cctgccttac tggaacgatg
      541 tgatcgcccc gcaggtccaa gccggcaagc gcgtgatcgt ggttgcgcat ggcaacacca
      601 tccgcgctct ggtgaagtac ctcgacaatg tgccggagaa agacatcgtg gcgctgaaca
      661 ttccgaccgg tattccgctg gtgtacgagc tggacgacca cctgaagcca ctctccagtt
      721 actacctcgg cgaccaggag aaggccaggc aggcggccgc cgcggtggcc gcccaggcgc
      781 gcgccggaac ctgagcccgg agcgcgtgcg acgatccgca ttcatcgcct ttctcgctgt
      841 tgaagccggc gcggcgctgc tgtttgccct tgccgccccg gcccatgccg cgcccaagga
      901 cgaactcgag gaactgcgcg gccgcatcgg ggcgctgcag aaacaactcg cggagtccga
      961 agagtcgaaa gccgaggccg gcgacgcgct cagggaatcc gaacgggcgg tcagcgaaac
     1021 caaccgcaag ctctatcaac tcgcggacca gcggcaccac gtcaatgccg cgctggcaaa
     1081 gttgcggtcg cgggcgcgca cggtcgagag cgatatcacc ggagagcagg ccacgctgag
     1141 caaactgctc taccagcagt acgtgaccgg ccagtcggac gcgatcaagc tggtgctcaa
     1201 tcgcgaggat ccaaacgaaa tcgcgcgcca actgcactat ctgggctatg tttcccgcgc
     1261 ccgcgccgaa ctgctggcca acctcaggta caacctcgcg aacctcgaaa agctggccga
     1321 cgaaacccgt gccaagagcg ccgaattgcg ggcactgcag aacgaagagg caagccagaa
     1381 acgccggctg gaaaaggaga aggtcgagcg ccagcgcgtt tacgcccagg tttccagcga
     1441 gatcgcgcaa agccgcaagc agttgtcaac gctcaagcgc gacgaggagc gcctgacgcg
     1501 cctgatcgag cgtctggcca aggaattcac gcgcaagaag ccgggcaagc gcatcgccaa
     1561 ccaggcgctg ccgagcgcgg atgcgggatc cgggccgttc cgcaagctca agggcaagtt
     1621 gaggctgccg gtactcgggg aactcatgaa ccgcttcggc agcccaaggc aggacagcgg
     1681 attgtcgtgg aagggtttgt tcatctctgc cgaaccgggc cgggaggtca aggccatagc
     1741 cggcggccgg gtggtctatg ccgactggct gcggggtttc ggtaatctca tgatcgtgga
     1801 tcacggcgac ggctacatga gcctgtacgg caacaatgaa gcgttgctca aacaggtggg
     1861 cgacgaaacc aggcccggcg acacccttgc ggccatcggc aacagcggtg gcaacccgga
     1921 ttcaggttta tacttcgaac tcagatatca gggaaaaccc ttcgaccctc tgccctgggt
     1981 gatattgaag taagttcttg attaggggca actccctgcg cacaaaggag caagcaaaga
     2041 tgcatagcag atttcggcag gtcggcctga tcgttctggg cgcgatcctc ggtgtgctga
     2101 tcagcctcaa tttctccgcc gtggcgcaga agggggcggg tcccctgccg atcgaggatt
     2161 tgcgcgcgtt taccgaagtc ttcggccgga tcaagaacga ctacgtcgag ccggtggacg
     2221 acaagcggct catttccgag gccatcaacg gcatgctctc cggcctcgac ccgcactcgg
     2281 ccttcctgga cgccgaagcg ttccgcgagc tgcaggtggg cacgcagggc gagttcggcg
     2341 gtctcggcat tgaagtcggc atggaagacg gcttcgtgaa agttgtcgcc ccgatcgagg
     2401 acacccccgc atggcgcgca ggactgaagt ccggcgacct gatcatcaag ctcgacgaca
     2461 ccaacgtgaa aggcatgacg ctgaacgatg cggtaaagcg catgcgcggc aagccggaca
     2521 gtcagatcac gctgaccttc attcgcaagg gcgataccaa gccgcgcgtg gtgacgctga
     2581 cccgtgccat catccagatc cagagcgtca aatcgaaact gctcgagccc ggctacgcgt
     2641 acttccgcgt cacccagttc caggagcaca gcgccgagac gctggccaag gcgatcgaca
     2701 agcatttcaa ggaaaaccag gggccgatga aaggcatcat cctggatctg cgcaacgatc
     2761 cgggcggctt gctcaacggc gcggtggcga tttccgcggc gtttctgccc agcgatgcgc
     2821 tggtcgtata cacggagggc cgtaccgaag acgccaagat gaagctgtac gcgcggcccg
     2881 agtattacct gcggggcagc aaggacgact atctgaagaa gctgccgaag gaagtgaaaa
     2941 cgctgccgat ggtggtgctg gtcaacagcg gctcggcttc cgcatcggag atcgtggcgg
     3001 gggcactgca ggaccataaa cgcgcggtgg tgatgggcac gcagaccttc ggcaagggtt
     3061 cggtgcagac cattctgccg ctcggcaacg gcaccgcgat caagctcacc acggcgcgct
     3121 acttcacgcc caacggccgt tcgatccagg ccaaaggcat cacgcccgac atcgtggtgg
     3181 aagaggcgac cctgaccgag gctgacgccg ccttgaaggt gcgtgaatcc gatctgcagc
     3241 gtcacttgtc caacccggac ggcagcgcca tcgagaacaa gcaaccggcg ccgcagccgc
     3301 agtcggcagt accggtgccg gcacccaagg ccggtgagaa ggccgccgcc gaaagcccgc
     3361 cggatatcgt gtcgaagagc gactaccagc tcaatcaggc gctcaacttg ctgaagggtc
     3421 tgcagatcct cagcaagaaa taagcttccg tccgaacgat cgaatggcaa gcaaggcccg
     3481 cccagcgcgg gccttgccgc gttaagatgg ccggaatcac gcccttgcca gcgatggacg
     3541 acgaccagtt actgcgttac agccgccaca ttctcctgcc cgagatcggc atcgacgggc
     3601 aggagaagct gctcggcgcg cacgcgttgc tgatcggcgc cggtggactg ggcgcgccgg
     3661 cgtcgcttta ccttgccgcc agcggcgtgg gccgcatcac cctctgcgac cacgacgcgg
     3721 tggacctcac caatctgcag cggcagatcg tgcatttcac cgaatcgatc ggcaggaaaa
     3781 aagtcgattc cgcgcgtgcc acgctggccc gcatcaatcc cgaaatctcg atcgaggcga
     3841 tcgccgaacg cgtcgccggc ttgcggctcg agcaactggt gcagtccgcc gacgtcgtga
     3901 tcgacgcgac cgacaacttt gccacccgcc atgcggtcaa tcgcgcctgc gtgaagttcg
     3961 ccaggccgct ggtgtccggt gccggcgtgc gtttcgacgg gcagatcgcg gtattcgatt
     4021 tgcgccgccg ggacagtccc tgttaccact gcctgttccc ggaaaacggc gacgtggagg
     4081 aaatgcgctg cgcggtaatg ggcgtgttcg cgccgctggt gggggtcatc ggctccatgc
     4141 aggcggcgga agcgctgaaa ctgctgatgg gaatcggccg cgatctgacc gggcggctgc
     4201 tgctgctcga cgcgctggcg atggagtggc gcagcgtcag gctgaagaag gattcgcagt
     4261 gcgccgtctg cggcggcggc taagattcgg tgattaagtg attgggcgat tcagtaactc
     4321 agcgattaag tgattaagta actcaatcgc ccaatcgctc aatcgcccaa tcgctcaatc
     4381 gcccaatcgc ccaatcgctc aatcgctcaa tccctcaatc cctcaatcac tctttctata
     4441 ctccaccctt gtccgcccgt tggcggcgga gacgtagatc atttccttgc tggtgacttc
     4501 cctgagcttc caggcggccg cgaaaccgac cggcatcagg ctgggcagcg tggaagcttc
     4561 gatcctggtg tgcaggtagc cttcgcgcac cgtccagttg ccgctcgccg tgaagtagta
     4621 gatcagcctg ccattgtcca cttcaaaatt gggcctgttc ggcaaataga agtccggcgg
     4681 aaccagcaga cgcacctgcc cctgccagtt gacccgccct ccgggcagca gcgtgaactg
     4741 ggtctcgaca atcacgtcgt ccggtgccac ttcccgcgaa ccccatgtcc ccaccagaac
     4801 tttcgcgaac cggtcgtccg acgcgccgca gccggacagc gccacgcctg cgcacatcag
     4861 gaagaaaaca gccgcgatgg tccgcgcggt gcgattaagc cgggcgcaac cgtccatcaa
     4921 acaatgcttg gccactgcgc cgaccagtac tcggtcggat cgcgcctgaa accgctcttc
     4981 gcgaactggt gccagcggcc taccacgaaa gccagcataa gattcgcctg cgcagtggga
     5041 tccacacctt cggggatttg cttttccgaa agcgcaaaac gcaacgcctg cttgagcgtg
     5101 gcctcgagcc ggtcgtgcaa ctggttgatg cgcgcctgca ggcgctcgtc ctcgttcacc
     5161 agcgcatcgc cgatcagcac ccgcgtcatt ccggggttct tcttggcgaa gccgagcagc
     5221 gtggtcagca cggcttcaat ctgcttcagg ccgctcttct cttccgcggt gatcttgttg
     5281 accagcccga acagggtctg ctctatgaac tcgatgaggc cctcgaacat ctgcgccttg
     5341 ctggcgaaat gccggtacaa cgccgcttcg gagacgtcca gtcgcttggc caacccggcg
     5401 gtggtgattt tctcggcttt gggatcctcg agcatggccg cgagggtctg caggatctgg
     5461 agcttgcgtt ctcccggctt ggtcggcatg ttgcggaggg atggctaggg atgagtaagg
     5521 gaaaggttac ccgaacgccc gggaatttaa cacgatccag cggcgtttgt tcgcgctcag
     5581 gacaaacact taccacggag gacaagcact aaccgcggag gacgcagagg acgcggagga
     5641 aagacaagag caaaaacatg cttgggttga aatgtttcat tggctaatga tccaccgcag
     5701 tgatcgttgg cgccaagtgt tcgcgaacag tctctccgct cttaacccca ctttgctttt
     5761 cctcgttttt gttcttcctc cgcgtcctct gcgtcctccg tggtaagagc aggtctagag
     5821 caggtgcagc agccgcggca gccgtacgat gtcacggaca ttcgcgtcca cccaggcggg
     5881 cgagcggctc gaccgatcca cccagacggt cttcattccc aggcgtttgg cggtgcgcag
     5941 gttctcgagg ctgtcctcga ccatgatgca gcgcgacgcc agcagccggt gcgtgcgcag
     6001 cagtctgcgg aatccatgcg catcgggctt gggccggaag cgggtgcgct cgatcgagaa
     6061 cacgtcgtcg aacaggtcgt ccacacccag cgccgccagg atcgcccgcg agtaatgcgc
     6121 cggggcgttg gaaaaaacga tcttgtgccc gggcaggcgc cgcaacaccg atcgcagttc
     6181 gcggcggccc accaccaggt ccccgatttg agggaatcgg tgggtttcgc gcaggaaatg
     6241 atgcgggtcg atgccgtgat ggcgaatcag ccccaccaat gtggcgccat agcggcgcca
     6301 gtagaactgg cgcagttcgt tggccgcata ctcgctcaga tccagatgct cggcaagata
     6361 agccgtcatc gacctatcga tgtggggaaa gaggtgcggg cgggcgtcat gcagggtgtt
     6421 atccagatcg aagatccagg tcctgccgct gaagcgggat tgcacagcag ctaccggttg
     6481 cggatcagcg tacccacgcc ctgatcggtg agcacttcca gaagcagcgc gtgctcgacc
     6541 cgcccgtcga tgatgtgcac gctctttacc ccgctcttgg cggcatcgag cgccgatgcg
     6601 atcttcggca gcatgccgcc gtggatggtg ccgtcggcga acagtccgtc cacttgcttg
     6661 ggcgtgagtc cggtcaaaag attgccgttc ttgtcgagca cgcccgctgt attggtcagc
     6721 aggatcagct tttccgctcg cagtatctcg gcaagcttgc cggcaacgag gtcggcattg
     6781 atgttgtaag actggccgtc ttcgcccacg ccgatcggcg ccaccaccgg aatgaattcg
     6841 cggctctcca gcagcgagat cacttccgga tcgatgctct cgatctcgcc cacctggccg
     6901 atgtccagca tttcggcagc gttgtcctcg ttctgcacca gcatcttcct ggcgcggata
     6961 aaggacccgt ccttgcccgt cagcccgatg gcacggccgc cgtgctggtt gatcaggttg
     7021 acgatgtcct tgttcaccag cccgccgagc accatctcga ccacgtccat agtctcgcgg
     7081 tcggtgaccc gcatcccctg gatgaattcg cccttcttgc cgacgcgctt caggaaatcg
     7141 tcgatctgcg gcccgccgcc gtgcaccacc accgggtcca tgccgaccag cttcagcaac
     7201 accacgtcgc gcgcgaacga gtgcttcagt ttctcgtcgg tcatcgcatt gccgccgtac
     7261 ttgatgacga tggtcttgcc gtggaagcgg cggatgtagg gcagcgcctc ggcgagaatc
     7321 tgcgccttgt gcgtggcgtc ggagggagaa aggggcatgg tgcgctcgct tggtcgtgga
     7381 gcgcgaattc taccgcggga aattcagctt cagagaaaaa acaaaggcgg ccgcagccgc
     7441 ctttgcccgg gcaaacacgc aattactgga tcgagagttt ctttgcagtc accgcagcct
     7501 tcttcggcag ccgcagctcc agcacgccat cgttgtactt cgcctgcgcc gtggcttcgt
     7561 ccacgtcctg ctccaggctg aaggcgcgat acgccttgcc gtagtaacgc tcggaacgca
     7621 ggaccttttc accctgcttg tcctggcgct cgcgtttcac ctcggcgctg atcgccacct
     7681 ggttgccgtt gatggtgacg ttgatgtctt cctttttcac gcccgggatt tcggcctgga
     7741 cgacataggc accatcgtct tccttcacat cgacgcggaa ttgcccctgc acctggtttt
     7801 cgaaggtcat cgggcgcacg aaaaagccct tgaagagatc gtcgaaagtc tcgtcaagcg
     7861 ggtagctgta acgggccaaa cttgccatat ctgtctcctt tgcgattagg gattgtgtcg
     7921 ggcgtcgggc gcccatgccc gctatatgtg cgcgggcggg gccttttcaa ggccttcatc
     7981 agggccggcc ggcaccctgt ccgcgcgccc tggcatcact cgcgcgcgcc gtgtgcgcct
     8041 gcgccgcccg gacggcctcg actgcatcca ggcgccgtgg cgcggcgtac ctgtcaacgc
     8101 ggtacgcgcg gcggcatcgg tccgcgccga gcccggcctg ccagccggcg ggctgggcaa
     8161 ccggggcaat tcatccatca ctccacaggg ggaaccactt atggaaggca agaaactcgc
     8221 cggtatggcg ctcgcggcaa cggccgccgc tctgttcatg acaggttgtg caaacatggg
     8281 cggcggtagc ggaacctccg cgaaagtgca ttgcgaaggc gcgaatgcct gcaagggcat
     8341 gagcgactgc aagtccgcga acagctcctg ccatggccag aacgcctgca aggggcaggg
     8401 cttcgtttcg ctcacgccgg cggaatgccg gaaggtctcg ggcagggcgt aaacttggcc
     8461 gacccaggcg ccgcggggca tccattgctg cggatgcccc gtttttcgct catcatgcaa
     8521 tgtccatgac tgcacaacct gcgctcggct tcgggctggg cctgcgcacc gaccattacg
     8581 aggacatcct cgcgtcgccg cccaacgtcg actggttcga ggcgctgacc gaaaactacc
     8641 tggtgccggg cggcaagccg ctgcattacc tcgaccg
//
LOCUS       DASQOP010000230        25835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2323331, whole genome shotgun sequence.
ACCESSION   DASQOP010000230 DASQOP010000000
VERSION     DASQOP010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 25835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25835
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2323331"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            440..1723
                     /locus_tag="VE030_09240"
     CDS             440..1723
                     /locus_tag="VE030_09240"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZF23636.1"
                     /translation="MNRFLRSFGATTAFFAAALLGTPFASAQAPLRIGVITFLSGPAA
                     GPFGVPAKNASDVLMDALNKGGQIPGYERKGFGGRPLEVVYVDEAGGPTKVVTEYRNL
                     VARQDVDMVIGVISSGDCLAVAPVAEELKQFTVLFDCATNRIFEEASYKYVFRTTTMA
                     TQENVAAARYIAESFPNLKTFSGVNPNYAWGQDAWSDFTEAMKIIKPQVQIGTNVTPK
                     LGAGQYGTEVSAVLGSGAEIVQNSLWGGDLEAFILQAAPRGLLARMPNIMITGETVLD
                     RLGDQLPDGTMIGARGPNGPFAPPTTLNKWFQKAFKERFGVAPVYASYHMATAFMGAK
                     AAFEKAQAANKVLDPTIDQIIGAFEYLKFESVAGTVDMSLGKGHQAVQHVPYGTVRKV
                     DGKLTLTNIKYYTPEHISPPDGMKSIDWIRSGFKR"
     gene            1827..2897
                     /locus_tag="VE030_09245"
     CDS             1827..2897
                     /locus_tag="VE030_09245"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HZF23637.1"
                     /translation="MKEFNIALIPGDGIGKEVVPEGTRVLDAAAKAHGGLRFSYRSFP
                     WSCEYYQKHGRMMPGDGLDQLRPFDSIFLGAVGFPGVPDHVSLWELIAPIRRGFQQYA
                     CVRPVKLLPGVKTPLADRGPQDIDFVVVRENNEGEYSNMGGRIYSGTPNEVVSQISIF
                     TRMGTERIIRYAFELARRIGKKKVTSATKSNGINFSMPFWDEVFWQIAKGYPEIESEQ
                     MHIDALSARFVTHPQRFGVVVGSNLFGDILTDLGAAIAGSIGIAASANINPERQYPSM
                     FEPVHGSAPDIAGKGIANPLGQIWSGAMMLDHLGQAELASRIVSAMSGVMQSGLRTPD
                     LGGNANTRQVTDAVVAAILKAG"
     gene            2961..4238
                     /locus_tag="VE030_09250"
     CDS             2961..4238
                     /locus_tag="VE030_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZF23638.1"
                     /translation="MSKKTERTIAPGRRKLIKQTATLTVAAAAAQFGFPSILRAQSEA
                     IRIGHITPRTGFLGQVGEYGYKGAMLAVEEANAAGGVLGRKVEMIAEDSVNPATAVTK
                     ANKLVERDQVICLIGEISSASGLAIGEQAQRYKIPFFNTGCNSDALRGANCKRYMFHI
                     EGSNTMYTKTIGTWQLQKNLIRGARWCFLTADYAFGHDLYKVSSRFLEQNGGIILNND
                     FVPTNTADYSAYILKIRQLKPDFVYLNLAGVDQTTFLKQYKEYGLPYQLAGGVMDTAP
                     FWATGLDALSGHWQSLWYHGLNIPASRAFTERFVKKYGQPPENQAWGDYIGVRIVLQT
                     VAETKGTEAARIVQHLEKGAEFDLLKARKGKFRDWDHQLLQEMYVVNVKDKSKSKDQW
                     DIFDIVETVPGPKESLELIQPTQQENPCSLPAA"
     gene            4263..5129
                     /locus_tag="VE030_09255"
     CDS             4263..5129
                     /locus_tag="VE030_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZF23639.1"
                     /translation="MLLLEQIANGLLVGSYYIVLSLGLSLIFSLGGVVNLAHGAFYAV
                     GAYLAYEIERRLGFLGAMGLSPVGVALIGILIERFALRRLYSRDPMLGLLFTFGLALT
                     AEQSLRMIWGTTGLPFSLPEQLRGQLILGDFIYSYYRLFVLAVAITAVTGCWLLLNKT
                     AFGMVVRAGVRDPEMVRALGISLRPILTAVFALGVALAGLAGVLSAPLAGVQPAMGTE
                     ILTATFVVVVIGGLGSFWGVVISGLLVGAVRGITVYYYPPAAEASMYLLMVLILLFRP
                     RGLMGEKFEKFE"
     gene            5126..7660
                     /locus_tag="VE030_09260"
     CDS             5126..7660
                     /locus_tag="VE030_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HZF23640.1"
                     /translation="MKPGNFLRHPLVISAVALLALPHVVTGTGFTWGIATEIAIFALV
                     GLGYNLLLGYTGLLSFGHGMFFGLAAYATALSQMHWFKGSLWLPLGFAVLCSAAFGLV
                     AGFLVLRRRGVYFSLLTLAFTALTFYIVYRWTSFTGGENGLSGIQRAPIFGLDLDNRV
                     VFYHLIAVIVFLVAWIMWRVVHSPLGSVLAAIRENENRARFIGYPVLRYKLAAFVISA
                     TVTGLGGSLMAFLKLFVSADTVHVNFSGEILAMTIFGGVGSFLGPTLGAFFYIMFREV
                     LTGYTSAWQFWFGLLFMAFILFSPSGLVGVGARLLAPLRKKTEGVGAMAARVTPQPGQ
                     EIPAFLCEHSPVSGTILNCAGVVKRFGGFTAVAGVDLTLADRKLHALIGPNGAGKTTL
                     FNTLSGMFAPDAGHIVLVNTPIAGLPAEQVVKQGLARSFQITSLFPALSVWENLRLAV
                     QARDARRFNVWTRSNALPQVNVETRQLVKFLGLENTEHALISSLSYGGQRLVEIGMAL
                     AAKPRVLLLDEPLAGLAAAERERIGVLIRRLTGDMAVLLVEHDIDRVFGMADTVTVMN
                     EGRVLVEGDAETVRAHPEVQRVYIGSGHAHLAAHRTQTDPSSQKVLLQLDKINSFYGK
                     SHILHDVSLLVRARDVVGLLGRNGAGKSSTFKSILGIVQPASGTVTFNGQVISGSTPE
                     RIARSGIGLVPQGRRLFSGLTVEENLRLGALARQAGDGVMWDRDRIYGYFPRIREKLH
                     VKADLLSGGEQQMVAIARALSGNVKLLLMDEPFEGLSPAMVDEVFKSIEQLREEVAIL
                     IIEHHLDLVLSLADRAYVLDRGHVSHEGPARPLLTDLDFRKQVLWL"
     gene            7832..8608
                     /locus_tag="VE030_09265"
     CDS             7832..8608
                     /locus_tag="VE030_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZF23641.1"
                     /translation="MEISAKGKRVLITAGGAGIGRAMTDVFHKAGARVHICDVVQASL
                     DETLEAFPGVSATLADVSSLKDVDRLFADLKKRLGGLDCLINNAGIAGPTGKVEDIAV
                     EAWERCIAVDLNGMFYCTRLAVPMIKAAGGGSIINLSSAAGRLGFPFRTPYAAAKWGV
                     VGFTKSLSMEVGPDNIRVNAIQPGVVEGSRINRVIEAKAKAVGISFEEQRKISLERVS
                     MHRMVSPYDIANMALYLASDIGGNVTGQALSVCAGVEMLG"
     gene            8752..9687
                     /locus_tag="VE030_09270"
     CDS             8752..9687
                     /locus_tag="VE030_09270"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HZF23642.1"
                     /translation="MEKVAIIGAGLIGRAWAMVFARAGHPVKLYDSTPGATDKAIGLV
                     REGLEELQQFGLIKDSPDAVMKRVSGAATLAEAVRDADYVQENTSERLDVKREVYRQL
                     DEAAPAHCILASSTSTIQTSRFSDGLKGKHRCIVAHPVNPPHVVPIVEISPAPWTSPD
                     VVARTRALHDKVGQVAITVKKEVEGFILNRLQAALLLESWRLVKEDYVSVEDLDKCVK
                     DGLGLRWSFMGPFETIDLNAPGGVSDYAARYGQVLYGMMKDLEHEPWDNKLVARIEGE
                     RRKLMPQTQHAEREAWRDRRLMALIAHKRSMEPKD"
     gene            9899..10825
                     /locus_tag="VE030_09275"
     CDS             9899..10825
                     /locus_tag="VE030_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HZF23643.1"
                     /translation="MANKVIITCAVTGAIHTPSMSPHLPVTPDEIADAAIGAAKAGAA
                     IVHLHAREPKDGRPTQDPKMFLRFLPKIKAASDVVVNLTTGGAPTMSVEERLQPALQL
                     KPEVASLNMGSMNFGLYEMLQRFTEFKHDWEKPYLAGSDDRIFKNTFKDIAHILESCS
                     NNDTRFEIECYDIGHLYTAAHFLDRGLVKPPLFIQSVFGIRGGIGPHPEDVLHMKRTA
                     DRLFGEQYQWSVLGAGRNQMYIATQSAVMGGNVRVGLEDSLWIGKGQFAKSNADQVSK
                     IRRILEELGLEIATPDDARKILKLKGKNNVNF"
     gene            11006..12085
                     /locus_tag="VE030_09280"
     CDS             11006..12085
                     /locus_tag="VE030_09280"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007588794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HZF23644.1"
                     /translation="MKSYKIAAIPGDGIGTEVIAAGIEVLEMLAKQDGSFKLRFEHFD
                     WGSAYYLKHGEMMPADGLARLRNFDAIYFGAVGSREVPDHITLWGLRLNICQGFDQYA
                     NVRPARVLPGIEPPLKLKSPADLDWVIVRENTEGEYSGQGGRSHRGLPEEVATEISVF
                     TRAGVERIQRFAFGLARKRPKRHLTLITKSNAQRHGMVMWDEIFASVAKDYPEVETDK
                     MLVDAATMRMVLRPASLDVMVATNLHADILSDLAAALSGSLGIAPTANLNPERRFPSM
                     FEPIHGSALDIVGKGIANPIGTFWTAVMMLEHLGESGAAARLMGAIESVTQAHVFTPD
                     LDGKATTAQVTRALCKSIETLPIHA"
     gene            12415..14223
                     /locus_tag="VE030_09285"
     CDS             12415..14223
                     /locus_tag="VE030_09285"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZF23645.1"
                     /translation="MKRKLAAAQQLLRETNEKLESASAELLISSERLSDSQAITRSTL
                     DALKATLDREKLALAFAEIGTCEFDVRSRMVRASEMSLRLFGIRSDHPDVPYKQLLES
                     IHIEDRPHFTNALAECLGGTGNLDIEYRVSWPDGTHRWLRTKGDALLDLEGEPIKVLW
                     VTEDVSQRKDMDARVRFLAHHDLLTGLPNRTLFQDRLQQALASAKRMETRVALLFIDL
                     DRFKNVNDSFGHRAGDILLQTVAARLRGCVRETDTVCRHAGDEYLIVLSALREPAEAA
                     LVAGKVLSTFEEPFLLDTQEIQISASVGISVYPDDGQTMEDLIRNADAAMYYSKKSGR
                     NRYEFFTPELTAPVAERLVLANQLRRAIERNQLVLHYQPQFDTANGKLLGAEALVRWT
                     HDEHGLLFPESFIPLAEESDIIHLIGEWVLNEACRQINAWQTDGLRVVPVAVNYSAFQ
                     FRRANLVQGVAAAFARHNLKPSQLEIELTENAIMQDPKETARTLQMLHEMGVSLSIDD
                     FGTGYSSLNYLKRFPIDKLKIDRSFVEDLPHDLNDSAIVQAIINLAKSLRMTVVAEGV
                     ETRGQLDFLRALGCEAYQGYFGGRPMEAERFAAMLA"
     gene            complement(14230..15738)
                     /gene="glpD"
                     /locus_tag="VE030_09290"
     CDS             complement(14230..15738)
                     /gene="glpD"
                     /locus_tag="VE030_09290"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HZF23646.1"
                     /translation="MDTLDSEYDLLVIGGGINGVGLARDAAGRGVRVALCERGDLAGA
                     TSMASSKLIHGGLRYLEQRQWRLVRESLAEREVLMRLAPHLVRPIRFVLPHVPGLRPK
                     WMIRAGLWLYDRLGGASTLPRSRAVDLRAPPFEDDLDPRFGSGFSYSDCRVDDARLVV
                     VNALSAQTLGAKILPRTACVGLTATGKGWRADLEAADGRRGFLSAKAIVNAAGPWVEQ
                     LLREIAGLQVQARVRLVKGSHIVVPRRIAGDHAYILQNRDRRIVFLLPFERRFTLIGT
                     TEVALAQPPAVMEVSDEEVRYLCDAANRYLRTPINAGDVVWCYAGVRALHDDGKASST
                     EVTRDYVLQLDHSASGAPILSVFGGKITTYRRLAEHALDKLSSVLELPGRPWTATAPL
                     PGAATGHFDYVEFLKSMLTRCAALPTGLVEGIVRRHGSTAIDLIGDAQNVSDMGTHFG
                     AGLTAREVDHFIDREWARTAEDVLWRRSKIGLHLSAAERAAVEDYITRRISA"
     gene            complement(15702..15890)
                     /locus_tag="VE030_09295"
     CDS             complement(15702..15890)
                     /locus_tag="VE030_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23647.1"
                     /translation="MSRRIQVLQGDITRQQVEAIVNAANASLLGAERSLGWAALTRCI
                     PRGRSKRWIRWTANTIFW"
     gene            15980..16705
                     /gene="ompR"
                     /locus_tag="VE030_09300"
     CDS             15980..16705
                     /gene="ompR"
                     /locus_tag="VE030_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HZF23648.1"
                     /translation="MNAAITNILVVDDDQRLRDLLNRYLAEQGFGVHAVANGSAMERV
                     ISKENVDMIILDLMLPGEDGMSICRRLRAGGRRTPIIMLTAKGDEADRIAGLDVGADD
                     YLPKPFNPRELVARINAVLRRQSPASALPGAPDDSKAPVRFGSFEINFATRTLTRNGK
                     HVGLTTGEFAMLKALVTHSRTPLSRDRLMELARGREYGVFDRSIDVQVSRLRRVLEED
                     PANPRYIQTVWGVGYVFVPDDSR"
     gene            16722..18050
                     /locus_tag="VE030_09305"
     CDS             16722..18050
                     /locus_tag="VE030_09305"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZF23649.1"
                     /translation="MNWIPQSLLSRTVLVISALLVVSQLIWLGLYRSYNVRIQSEYLA
                     NQIASVIGTVSVALETMPPAVRRKFSERLPAHHNIKLLPATAWDDEDVPLPQSPLLRA
                     IGEALRREVADDAQALVLLEDATRALWIKIKVKNQAYWVVFPPESRGSASAWAWTGWS
                     VFGLTLAIAGGLLLVLRVNRPLRALASAAEQIGAGRTPPPLDEQGPTEIRTLSHAFNQ
                     MTSNLKQLDNDRAVLLAGISHDLRTPLSRLRLGIEMLGEAGDHGLKNSMIQDIEDMDA
                     IVNQFLDFAREGGNEPSRNGEDLNHIVSSVCERFSRMGKSVNTNLGELPPLTLKPTAT
                     QRMVTNLVDNALRYGGGEVEVRTECVDGHALVRVLDRGPGIPESEVWRVMQPFTRLEA
                     SRSGGNGSGLGLAIVERAARMNGGNVKLLRREGGGLEARVELPTDTQKAA"
     gene            complement(18227..18766)
                     /gene="thpR"
                     /locus_tag="VE030_09310"
     CDS             complement(18227..18766)
                     /gene="thpR"
                     /locus_tag="VE030_09310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HZF23650.1"
                     /translation="MNLHNLRVFYALWPDGNVNSDLAETARRMHRTVGGRRTRDDSIH
                     LTMAFVGDVDGARLAELLVPPAELTVTRFTLTLDRWGCWPRNGIAWVAPSRVPDPLRE
                     LAARMEAWLRDAGFEMDARPFNPHVTLVRKAECAPLPDSTPIIDWQVEDFVLVRSTLA
                     PDGSRYETIGRWPLAGVHS"
     gene            complement(18763..19260)
                     /gene="ispF"
                     /locus_tag="VE030_09315"
     CDS             complement(18763..19260)
                     /gene="ispF"
                     /locus_tag="VE030_09315"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HZF23651.1"
                     /translation="MRIGQGFDVHALVAGRPLILGGVAIPYEKGLAGHSDADVLLHAL
                     CDALLGAAALGDIGRHFPDTDPAYRGADSRWLLREVAALLEKNHFHVLNVDTTIVAQA
                     PKMAPHIPAMIANIAADLAIARERVNVKAKTTEKLGFAGRGEGIAAEAIALIESRPPV
                     EIGHR"
     gene            complement(19439..20128)
                     /gene="ispD"
                     /locus_tag="VE030_09320"
     CDS             complement(19439..20128)
                     /gene="ispD"
                     /locus_tag="VE030_09320"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HZF23652.1"
                     /translation="MSRYLALIPAAGAGSRMGQPLPKQYLPILNRPLLHYSISCLCAH
                     ALVERVYVVLAAADRWFNEYDWDPLGAKLMPIYAGGESRAETVRNGLQRIEREVSGDD
                     WILVHDAARPCVTPALLDRLIGEIGADQTGGLLAVPLADTLKRANSIARVVATEARAG
                     LWQAQTPQMFRYDLLTRALQCAGLADITDEASAIERLGMQPRLVASDITNLKVTYPED
                     LALAELILKHM"
     gene            20298..20633
                     /locus_tag="VE030_09325"
     CDS             20298..20633
                     /locus_tag="VE030_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HZF23653.1"
                     /translation="MTLRYHLVLALLAACALGAAPASAQETYTIVIKDHRMNPAELEV
                     PAGQKIKLLVDNQDPTPEEFESHSLNREKVIPGNSKAAIFIGPLKPGSYEFFGEFHQA
                     TAQGRIVAK"
     gene            20646..21440
                     /locus_tag="VE030_09330"
                     /pseudo
     CDS             20646..21440
                     /locus_tag="VE030_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010090450.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
     gene            22287..25703
                     /gene="mfd"
                     /locus_tag="VE030_09335"
     CDS             22287..25703
                     /gene="mfd"
                     /locus_tag="VE030_09335"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HZF23654.1"
                     /translation="MLLPPLIPTPGSRATFPPLHGSADALALAELARSARPLAIITET
                     AQDAERLREEIAFFDPTLRVFLLPDWETLPYDSFSPHQDLISERLATLYQITHDAFDV
                     AVVPITTALYRLPPAAYLAAYTFFLKQGDKLDANSMRRQLTLAGYSHVTQVVSPGEFS
                     FRGGVIDLFPMGSPLPYRIDLFDDEIESIRSFDVDTQRSIYKVKEVRLLPAREFPTDD
                     NARTTFRQRFREKFEGDPSKSRLYKDMSQGFVPAGIEYYLPLFFESTATLADYLPAGT
                     LVSLHQNVAKAISQFWRDTQSRYNLLRGDPDRPLLPPAELFLREDEFFGSLKPFPRIE
                     TASAANSESAAAPAPTQALPPVAVDRRADNPLAALSAFHGNYGGRVLLIAESMGRRET
                     LLGLLNEYQVRPKVCDGWQAFKDASERMMLTVGPIHAGFVDSGDAVALITENELYPSQ
                     IRTRVSRERRIATEGMLRDLSEVKIGDPVVHAQHGIGRYLGLANMNLGDGETEFLTLE
                     YDGGDKLYVPVSQLAVIGRYSGAAPEAAPLHKLGSGQWEKARRRAAAQVRDTAAELLN
                     LYALRAARLGYAFELKPHDYEAFREGFPFEETPDQAAAIQAVIGDMGAGKPMDRLVCG
                     DVGFGKTEVALRAAFVAVADSKQVAVLVPTTLLAEQHFQTFSDRFADWPVKIAELSRF
                     RSAKETSGALEGLEQGRVDIAIGTHKLLSRGVKFKDLGLVIIDEEHRFGVRQKEKLKA
                     LRAQVDVLTLTATPIPRTLAMALEGLRDFSVIATAPQRRLAIKTFVSPLSEGLIREAV
                     LRELKRGGQVYFLHNEIETIRNMEARVAKLLPEARIRTAHGQMPERELELVMRDFYQG
                     RFNLLLCTTIIETGIDVPTANTIIINRADKFGLAQLHQLRGRVGRSHHQAYAYLLTPD
                     EDAIGSQARKRLEAIQMMEELGSGFYLAMHDLEIRGAGEVLGESQSGEMQEVGFALYA
                     EMLNAAVKSLKAGKEPELSGNFNPGTEINLHAPALLPEDYCADIHERLVLYKRLANCE
                     TPEELEAMHEELVDRFGLLPDPAKVLLETHRLRITARQLGISRIDAGPESLLLQFIPN
                     PPLDPAKIIGLVQSRKNFKLSGQDRLRVEAAMPDIAARVSQARAVLKDLG"
BASE COUNT         5037 a         8081 c         7889 g         4828 t
ORIGIN      
        1 attccgtcga agtgtcgagg gtgaggatgt tcaaggcgcg tccatcagcg cttaagaggc
       61 gcgaaacaag tgtcctgctc cgctgattcg tgggacaact tgcgaacctt ggagcgcaga
      121 ccccatatcc attggcaaca atccatcatt gatcggaaaa cccgcgaaat cggtgtcagg
      181 caaggcgcga gtccttgaca gtggcgggcc ccacggcgag gactcgtaac accgcgtggc
      241 gccgattctc gtcgggtttt cagccgcgaa ttagcggaac aggacactaa ccggccacgc
      301 ggattctagc ggaaccgaat cgccaaaagt tagttgttat aatcgcagcc tgtcagccgc
      361 ggcgggtttg tgaccgccag gggcggcggg cggcgaccgc gccgtcaaaa aacataaaga
      421 cccatccgga ggaccagtca tgaaccgctt cctgcgcagc ttcggcgcaa ctaccgcatt
      481 tttcgcagcg gcgctccttg gcacgccgtt cgcctcggcg caagctccac tgcgaatcgg
      541 ggtcattacc ttcctgtccg gaccggctgc cggtcccttc ggcgtaccgg ccaagaatgc
      601 ctcggatgtg ctgatggacg cgctgaacaa gggcgggcag attccgggct atgaaaggaa
      661 gggcttcggc ggaaggccgc tcgaagtggt ctacgtggac gaggccggtg gaccgaccaa
      721 ggtcgtcacc gagtatcgca acctggtggc acgccaggat gtggacatgg tgatcggggt
      781 gatttccagc ggcgactgcc tggcggtggc gccggtcgcc gaggaattga agcagttcac
      841 cgtgttgttc gattgcgcca ccaaccgaat cttcgaggaa gcgagctaca agtacgtttt
      901 ccgcaccacc accatggcga cccaggaaaa cgtcgcggcg gcccgctaca ttgcggagag
      961 cttccccaat ctcaaaacct tctcgggggt caatccgaac tacgcctggg gccaggacgc
     1021 ctggtccgat ttcaccgagg cgatgaaaat catcaagccg caggtccaga tcggcaccaa
     1081 cgtcacgccc aagctcggcg cgggccagta cggcaccgaa gtctccgctg tgctgggcag
     1141 cggagccgaa atagtgcaga actcgctttg gggcggcgat ctcgaggcct tcatcctgca
     1201 ggcggcaccg cgcgggttgc tggcgcgcat gcccaacatc atgattaccg gcgaaactgt
     1261 gctcgaccgt ctcggcgacc agttgcccga cggcaccatg atcggtgcgc gcggccccaa
     1321 cggtccgttt gccccgccga ccacactcaa caagtggttc cagaaggcgt tcaaggaacg
     1381 cttcggcgtc gcaccggtat acgcttccta ccacatggcg accgcgttca tgggtgccaa
     1441 ggccgctttc gaaaaagcac aggccgccaa caaggtgctg gatccgacca tcgatcagat
     1501 catcggcgcg ttcgagtacc tgaagttcga gtcggtcgcc ggcacggtgg acatgtcgct
     1561 cggcaagggg catcaggcgg tgcagcacgt gccttacggc acggtcagga aggtcgacgg
     1621 gaaactcacg ctcaccaaca tcaagtacta cacgcccgag catatcagcc caccggacgg
     1681 aatgaagagc atcgactgga tccgctccgg tttcaaacgt tagaaactat cacctcaccc
     1741 cccaccctct ccactgacgt ggagagggag ccctaagaac atatgttgac gctttgcaga
     1801 cgtgccaccg tgattaacac catcccatga aagaattcaa catagcgctg atcccgggtg
     1861 acggcattgg caaggaagtc gtccccgaag gcacgcgcgt tctcgatgcc gcggcgaaag
     1921 cgcacggcgg gctcaggttc agctaccgca gcttcccgtg gagctgcgag tactaccaga
     1981 agcacgggcg catgatgccc ggggacggac tggaccagtt gcgccctttc gattccattt
     2041 ttctgggtgc cgtcgggttt cccggcgtgc ctgaccatgt ctcgctgtgg gagctgatcg
     2101 cgccgatacg ccgcggcttc cagcaatacg cctgtgtgcg gccggtaaag ttgctgcccg
     2161 gcgtgaaaac gccgctcgcc gaccgcggcc cgcaggacat cgacttcgtg gtggtgcgcg
     2221 aaaacaacga aggcgagtac tccaacatgg gcggccgcat ctactccggc acgcccaacg
     2281 aagtggtcag ccagatcagc atcttcaccc gcatgggcac cgagcgcatc atccgctatg
     2341 cgttcgagct tgcgcgcagg atcggcaaga agaaagtcac ctcggccacc aaatccaatg
     2401 gcatcaattt cagcatgccg ttctgggacg aggtgttctg gcaaatcgcc aaaggctacc
     2461 ccgagatcga aagcgagcag atgcacatcg acgcgctttc ggcgcgcttc gtcactcatc
     2521 cgcaacgctt cggcgtggtg gtgggcagca atctgttcgg tgacattctc accgacctgg
     2581 gggcggccat tgccggcagc atcggcattg cggcttccgc caatatcaat ccggaaaggc
     2641 agtatccttc catgttcgag ccggtgcacg gctcggcgcc ggatattgcc ggcaaaggca
     2701 ttgccaaccc gctcggccag atctggagcg gggcgatgat gctggatcat ttgggccagg
     2761 cggaacttgc aagccgcatc gtgtcagcca tgtcgggggt gatgcaatcc gggctgcgca
     2821 cgccggacct gggcggcaac gccaatacca ggcaggtaac cgacgccgta gtcgccgcca
     2881 ttctcaaggc gggctgaacc ggatcggaat cgaaccggca acccgaaaaa agggctgcca
     2941 tagaaatcgg gggaggaatc atgagcaaga aaaccgaaag gacgatagca ccaggccgcc
     3001 gcaagctgat caaacagacc gccacgctga ccgtcgctgc tgctgcggcg cagtttggtt
     3061 tcccgtcaat cctgcgcgcg caatccgagg cgatccgtat cggccacatc acgccgcgca
     3121 ccggctttct cggccaggtg ggcgagtacg gctacaaggg cgccatgctg gcggtcgaag
     3181 aagccaatgc ggcgggcggc gtgctcgggc gcaaagtgga gatgatcgcc gaggacagcg
     3241 tcaaccctgc caccgccgtg accaaggcga acaagttggt cgagcgtgac caggtgatct
     3301 gcctcatcgg ggagatcagt tcggcttcgg ggctcgccat cggcgagcag gcacagcgct
     3361 acaagatccc gttcttcaac accggctgca attccgatgc gctgcgcggc gccaactgca
     3421 aacgctacat gttccacatc gagggcagca acaccatgta caccaagacc atcggcacat
     3481 ggcaattgca gaagaacctg atcaggggcg cgcgctggtg ttttctcacc gcggactatg
     3541 cattcggcca tgatctctac aaggtttcct cgcgcttcct ggagcagaac ggcggcatca
     3601 tcctcaacaa cgatttcgtg ccgaccaaca ctgcggacta cagcgcctac attctcaaga
     3661 ttcgccagct caagcccgat ttcgtctacc tcaatctagc cggcgtggac cagacgacgt
     3721 tcctgaaaca gtacaaggaa tacggcctgc cctatcagct tgccggcggc gtgatggaca
     3781 ctgcgccgtt ctgggccacc ggcctcgatg cgctgtccgg ccattggcag agcctgtggt
     3841 atcacggctt gaacattccc gcgtcccggg catttaccga acgctttgtg aagaagtatg
     3901 gacaaccgcc ggagaatcag gcctggggcg attacatcgg cgtgcgcatc gtgctgcaga
     3961 cggttgcgga aaccaaggga accgaagccg ccaggatcgt ccagcatctg gagaaaggcg
     4021 cggaattcga tcttctcaag gcgcgcaagg gcaagttccg cgactgggac catcagttgc
     4081 tgcaggaaat gtacgtggtc aatgtgaagg acaagtcgaa gtcgaaggat cagtgggaca
     4141 tcttcgacat cgtcgagacc gtgccgggcc ccaaggagtc gctggagctg atccagccca
     4201 cacagcagga aaacccctgc agcctgccgg cggcgtgagt cgcggctgag ttcgtttcag
     4261 ccgtgcttct tctcgagcag atcgccaacg gcctgctggt cgggtcgtac tacatcgtcc
     4321 tgtcgctggg actttcgctg atcttcagtc tcggcggtgt ggtcaacctt gcccacggcg
     4381 cgttctatgc ggtgggcgcc tatctcgcct acgaaatcga aagacgactc ggctttctcg
     4441 gcgcgatggg gctctccccg gtgggcgtgg cgctgatcgg catcctgatc gagcgctttg
     4501 ccctgcgacg cctctacagc agggatccga tgctgggcct gttgttcact ttcggcctgg
     4561 cgctgaccgc cgagcaaagc ctgcgcatga tctggggtac caccggcctg ccgttctcgc
     4621 tgccggaaca gcttcgcggc cagttgattc tgggcgattt catctattcc tattaccgcc
     4681 tgttcgtact cgcggtggcg atcaccgccg tcaccgggtg ctggctgttg ctcaacaaga
     4741 ctgcattcgg aatggtggtg cgcgccgggg ttcgcgatcc ggaaatggtg cgtgcgctgg
     4801 gcatctcgct gcggccgatc ctgaccgcgg tgttcgcgct tggggtggcg ctcgcgggcc
     4861 tggcgggcgt tctctcggcc ccgctcgcag gcgtgcaacc cgcgatgggc accgaaatcc
     4921 tcaccgcaac tttcgtcgtg gtagtgatcg gcggattggg cagtttctgg ggcgtggtca
     4981 tttccggcct gctggtcggt gccgtgcgcg gcatcaccgt ttattactac ccgcccgcgg
     5041 cggaagcctc gatgtacctg ctgatggtgc tgatcctgct gttccgcccg cgcggattga
     5101 tgggcgagaa attcgagaag ttcgaatgaa gccgggcaac ttcctcagac atccgcttgt
     5161 catctccgcg gtggcgctgc tggcgctgcc gcatgtggtc accggcaccg gcttcacctg
     5221 gggcatcgcc accgagatcg cgatctttgc gctggtcggc ctgggctaca acctcctgct
     5281 cggttacacc ggactgctgt cctttggtca cggcatgttc ttcggactgg ccgcttacgc
     5341 cactgcgctg tcgcaaatgc attggttcaa aggcagcctg tggctgccgc tcggcttcgc
     5401 ggtactttgc tccgccgcgt tcggcctggt cgccggcttc ctggtgctgc gccggcgcgg
     5461 cgtgtacttt tccctgctca cgctcgcgtt cactgcactg accttctaca tcgtgtaccg
     5521 ctggacttcg ttcaccggtg gcgaaaacgg cctttccggg attcagcgtg cgccgatttt
     5581 cggactcgat ctcgataacc gggtggtttt ctaccacctg atcgctgtga tcgtatttct
     5641 cgttgcatgg atcatgtggc gggtcgtgca ctcgccgctc ggctcagtgc tcgcggccat
     5701 tcgcgagaac gagaaccgcg cccgcttcat cggctatccg gtgctacgct acaagctcgc
     5761 ggccttcgtc atctccgcca cggtcaccgg cctgggcggc agcctgatgg cttttctcaa
     5821 gctgttcgtt tccgcggaca cggtgcacgt caatttctcc ggggaaatcc tcgccatgac
     5881 catcttcggc ggcgtcggca gttttctcgg gccgacgctc ggcgcattct tctacatcat
     5941 gttccgcgag gttctgaccg gctacacctc ggcgtggcag ttctggttcg gccttctgtt
     6001 catggccttc atcctgtttt cgccttcggg actggtcggt gtcggcgcgc gcctgcttgc
     6061 gccgctgcgc aagaagacgg aaggcgtcgg cgcaatggcc gcgcgcgtaa ccccgcaacc
     6121 gggtcaggag atccctgctt ttctgtgcga gcattcaccg gtttccggca cgatcctgaa
     6181 ctgcgccggc gtggtcaagc gtttcggcgg cttcactgca gtcgcggggg tggacctgac
     6241 gctcgccgac cgcaagctgc atgcgctgat cggacccaac ggcgccggca agaccaccct
     6301 gttcaatacc ctctccggca tgttcgctcc cgatgcggga cacattgtgc tggtcaatac
     6361 cccgatcgca ggcctacctg ccgagcaagt ggtgaagcag ggtctggcgc gctcattcca
     6421 gatcaccagc ctgttcccgg cgctgtccgt ctgggagaac ctgcggttgg cggtgcaagc
     6481 gcgcgatgcg cggcgcttca acgtctggac gcgcagcaac gccctgccgc aagtgaacgt
     6541 cgaaacacgc caacttgtga aatttctcgg gctcgagaat accgagcacg cgctgatctc
     6601 cagcctttcc tacggcggac agaggctggt cgagatcggc atggcactgg cggcgaagcc
     6661 ccgcgtcctg ctgctggacg aaccgctggc cggcctcgcc gcggcggagc gcgagcgcat
     6721 cggcgtactg atacgccggc tgaccgggga tatggcggtg ctgctggtcg agcacgacat
     6781 cgatcgcgtg ttcggcatgg ccgacaccgt cacggtaatg aatgaaggca gagtgctggt
     6841 cgagggcgac gcggaaaccg tgcgcgcgca tcccgaagtt cagcgcgttt acatcggcag
     6901 cggtcatgcg catctcgccg cccatcgaac gcagacggat ccgtcctcgc agaaggtctt
     6961 gctgcagctg gacaagatca attccttcta cggcaagagc cacatacttc acgatgtgag
     7021 tctcctggtg cgcgcccgcg atgtcgtcgg cttgctgggc cgcaatggtg ccggcaagtc
     7081 gtcaaccttc aagagcatac tcggcattgt tcaacccgca tccggcaccg tgactttcaa
     7141 cggccaggtg atcagcggca gcacaccgga acggatcgca cgatcgggca tcggcctggt
     7201 gccacagggt cgcaggttgt tctccggcct gaccgtggag gaaaatctgc gcctcggcgc
     7261 acttgcgcgc caggcgggcg acggcgtcat gtgggatcgc gacaggattt acggctactt
     7321 cccgagaatc cgcgaaaagc ttcacgttaa agccgacctg ctctccggtg gcgaacaaca
     7381 gatggtcgcg attgcccgcg ccctctccgg caacgtcaag ctgctgctta tggacgagcc
     7441 gttcgaaggc ctgtcgccag ccatggtcga tgaagtcttc aagtcgatcg agcagctccg
     7501 ggaggaagtc gcaattttga tcatcgagca tcatctggac ctggtgctgt cgctcgccga
     7561 ccgcgcctac gtcctcgacc gcgggcacgt gtcgcacgaa ggaccggcgc gcccgctgct
     7621 taccgatctc gatttccgca agcaggtgtt gtggttgtga ctgcaagatt caccacggag
     7681 acaccgaggc acggagaagg gccaactctc ccaactcgaa ggaaagcctg ccgctacgat
     7741 gctggatttc ttggcgctct tagtttgatt ctggttccag tttctttgtt gcatttctct
     7801 gtgtctctgt ggttagtaaa aggataaata aatggaaatt tcagcaaagg gaaagcgggt
     7861 cctgatcacc gccggtgggg cgggaatcgg ccgggcgatg acggatgtgt ttcacaaggc
     7921 cggtgcacgg gtgcacatct gcgacgtcgt gcaggcttcg cttgacgaaa cgctggaagc
     7981 ctttccaggc gtcagcgcca cgctggcgga cgtgtccagc ctcaaggacg tggaccggct
     8041 gtttgccgat ttaaagaaac gtcttggcgg gctcgactgc ctcatcaaca acgccggcat
     8101 cgccgggcct acgggaaaag tcgaggacat tgcggtcgag gcttgggagc gctgcatcgc
     8161 ggtggacctg aacggcatgt tctattgcac gcgcctggcg gtgccgatga tcaaggccgc
     8221 cgggggcggc tcgatcatca atctgtcttc cgccgcaggg aggctgggat tcccgtttcg
     8281 caccccctat gccgccgcca agtggggcgt ggtcgggttc accaaaagcc tgtcgatgga
     8341 ggtcggtccg gacaacatcc gcgtcaacgc gatccagcct ggcgtggtcg agggcagccg
     8401 catcaaccgc gtcatcgagg ccaaagccaa ggcggtcggc atcagcttcg aggaacagag
     8461 gaagatctcg ctcgaacgcg tctcgatgca ccgcatggtc agtccctacg acatcgccaa
     8521 catggcgctt tacctcgcca gcgacatcgg cgggaacgtc accggtcagg cgctgtcggt
     8581 ctgcgccggc gtcgagatgt taggttagat tcgaatccac cacagaggca cagagaagaa
     8641 caaggcgagc ttggattttc ttgaatccct ttttttctaa tcaggatctt gcatattttc
     8701 gttcttctcc gcgcctctgt gtctctgtgg taaatttttg aaggaaatag aatggaaaaa
     8761 gtagcaatca ttggtgcggg attgataggc cgtgcctggg caatggtgtt cgcgcgcgcg
     8821 ggacatcccg taaagctcta cgacagtacc cccggtgcga ccgacaaggc catcggcctc
     8881 gtcagggagg ggctggaaga actgcaacag ttcgggctga tcaaggattc tcccgacgcc
     8941 gtgatgaagc gcgtcagcgg cgcggccacg ctggcagagg ccgtgagaga cgccgattat
     9001 gttcaggaga acacatctga gcggctggac gtcaagcgcg aggtttaccg tcaactcgac
     9061 gaagcggctc cggcgcactg catcctggcg agctcgactt cgaccatcca gacgtcgcgc
     9121 ttcagcgacg ggctcaaggg caagcatcgc tgcatcgtcg cgcatccggt caatccgccg
     9181 cacgtggtgc cgatcgtcga gatctcgccc gcgccatgga ccagtcccga tgtggtcgcc
     9241 aggacccgtg cgctgcacga caaggtcggt caggtggcca tcaccgtgaa gaaagaagtc
     9301 gagggattca ttctcaatcg cctgcaggct gcgctgttac tggaatcatg gcgactggtg
     9361 aaggaggact acgtctcggt cgaggacctc gacaaatgcg tcaaggacgg actgggattg
     9421 cgctggtcgt tcatgggccc cttcgagacc attgatctga acgcgcccgg cggcgtgtcc
     9481 gactatgccg cgcgctacgg ccaggtgctt tacggcatga tgaaagacct ggaacacgaa
     9541 ccctgggaca acaagctggt cgcgaggatc gaaggggaac ggcgcaagct catgccccaa
     9601 acccagcacg ccgagcgcga agcctggcgc gaccggcggc ttatggcgct catcgcccac
     9661 aaacgctcaa tggaaccgaa agactaaaat cttcaccgca aagacgcaaa gaacgcaaag
     9721 aaaagcagaa aaatagccgc ggaaaacctg cactgcgcag aacctgcttt cgtcgcaagt
     9781 atcggctatc caggtatcga acgacatgaa ccgctttttt gcttgttatc cgggtttcct
     9841 ttgcgctctt tgcgcctttg cggtgacaga atctgactgc taactggaga aactgaaaat
     9901 ggctaacaaa gtcatcatca cctgcgcagt aacgggcgcc atccacacgc cttcgatgtc
     9961 gccccacttg ccggtgacgc cggatgagat cgcggatgcg gcgatcggcg cggcgaaagc
    10021 cggcgcggca atcgtgcacc tgcatgcgcg cgagcccaag gacggccgcc ccacccagga
    10081 tccgaagatg tttctgcggt tcctgccgaa gatcaaggcg gcatccgacg tcgtggtcaa
    10141 tctcaccacc ggcggcgctc cgaccatgag cgtggaagaa cggctgcagc cggcgctgca
    10201 gctcaagccc gaggtggcgt cgctcaacat gggctcgatg aatttcggcc tgtacgagat
    10261 gttgcaacgt ttcaccgagt tcaagcacga ctgggaaaag ccctatctcg ccggttccga
    10321 cgatcgcatc ttcaagaaca ccttcaagga catcgcccac attctcgagt cctgcagcaa
    10381 caacgacacc cgcttcgaga tcgagtgcta cgacatcggc cacctgtaca ccgcggcgca
    10441 cttcctcgat cgcggcctgg tcaagccgcc gttgttcatc cagtcggtat tcggcatccg
    10501 cggcggcatc ggcccccacc cggaggacgt gctgcacatg aagcgcaccg cggaccggct
    10561 gttcggcgaa cagtaccagt ggtcggtgct cggcgcgggt cgaaaccaga tgtacattgc
    10621 cacgcagtct gcggtaatgg gcggcaacgt gcgcgtcggc ctggaagaca gcctgtggat
    10681 cggcaagggc cagttcgcca agtccaacgc cgaccaagtc tccaagatcc gccgcatctt
    10741 agaagaactg ggcctcgaaa tcgccacgcc cgacgacgcg cgcaagattc tcaaactcaa
    10801 gggcaagaac aacgtgaatt tctagactgg ccgccaagac gccaagagcg ccgagaagtg
    10861 caaaagccat gacatgggaa acggcgaaat gccaagtatt ggtacagatg cgcagacata
    10921 cccgtcacgt tgcgcatcag gctcttgcaa attctctctt gtcttgcctt ggcgctcttg
    10981 gcgtcttggc ggcgaaagga tttagatgaa atcatacaaa attgcagcga ttcccggaga
    11041 cggcatcggc accgaagtga tcgcggccgg catcgaggta ctggagatgc tggcaaagca
    11101 ggacggcagc ttcaagctga gattcgagca cttcgactgg ggcagcgcgt attacctcaa
    11161 gcacggcgaa atgatgcccg cggatggtct tgcgaggctc aggaacttcg atgcgatcta
    11221 cttcggcgcg gtcggctcca gggaagtgcc ggatcacatt acactgtggg gcctgcggct
    11281 caacatctgc cagggcttcg accaatacgc caacgtacgc cccgcccgcg tgttgcctgg
    11341 catcgagccg ccgctcaaac taaagtcgcc ggccgacctc gattgggtga tcgtgcgcga
    11401 gaacaccgag ggcgaatact ccggacaggg tggacgttcg catcgcggcc tgcccgagga
    11461 ggttgctacc gagatctcgg tatttacccg tgctggcgtg gagcgcatcc agcggttcgc
    11521 cttcggtctt gcgcgcaagc ggccgaaacg gcatctgacc ttgatcacca aatcaaacgc
    11581 acagcgccac gggatggtga tgtgggacga gatctttgcc tcggtggcga aggattatcc
    11641 ggaggtcgag accgacaaga tgctggtgga tgccgccact atgcgcatgg tgctccgtcc
    11701 cgcctctctc gatgtgatgg ttgccacaaa tctgcatgcc gacatcctgt cggatctcgc
    11761 cgcggcgctc tccggcagcc tcggcatcgc gcccacggcg aatctcaacc cggaacgccg
    11821 ctttccgtcg atgttcgagc cgatccacgg ctcggcactc gacatcgtcg gcaaaggcat
    11881 cgccaatccg atcgggactt tctggaccgc ggtgatgatg ctcgaacatc ttggcgagtc
    11941 cggggccgcc gcccgcctga tgggcgcgat cgaaagcgtc actcaggcgc acgtcttcac
    12001 ccctgacctt gacggcaagg ccacgaccgc ccaggttacc cgggcgctct gcaagtccat
    12061 cgaaacactg ccaatacacg cctgattctg acaatcggcg ccgcgaagct gccgcggcgc
    12121 ccttgactcc tgccttgatt cacgggcgcc ttcccgccgg ccgtgttgcc ggtggggcgg
    12181 gaatcactca gaatgtcgat ggcacggcgc ttgcagcgat aggacgggac cctctcattg
    12241 cgaccagttc gcgactcccc gatcgcgccg agcatggaac aggaaacgac tgagtcccat
    12301 cgagtgagcc ccgccaaaag caaaagacgc gccgcaagca aggcaaagcc cggtaaacgc
    12361 gcaagtcctc gcgggcgcaa acccatcccg cgcacgttgg cgcaggctgc tgacatgaaa
    12421 aggaagcttg cagctgccca gcagcttttg cgggaaacca acgaaaagct cgagagcgcc
    12481 agcgcggagc tgctgatatc ttcggagagg ttgagcgatt cgcaggccat cacccgaagc
    12541 actctcgatg cgctaaaggc caccctcgac cgcgagaagc tcgcgctggc gttcgctgag
    12601 atcggcacct gcgagttcga tgttcgaagc cggatggtgc gcgcttcgga aatgagcctg
    12661 cgtctgttcg gcattcgcag cgaccacccg gatgttccct acaaacagct gctcgaatcg
    12721 atccatatcg aagatcgtcc ccatttcacg aacgccctcg ccgaatgcct gggcggaacc
    12781 ggcaacctcg acatcgagta ccgggtgtcc tggcccgacg gcacccaccg ctggttgcgt
    12841 accaaggggg atgcactgct cgatctggag ggcgaaccga tcaaggtgct atgggttacc
    12901 gaggacgtgt cccaacgcaa ggacatggac gcccgggtgc gctttctcgc ccaccacgac
    12961 ctgctcacgg ggctacccaa ccgcacgctg tttcaggacc gcctgcagca ggctctggca
    13021 tcggcaaaac gcatggaaac cagggtcgcg ctgctgttca tcgacctgga ccgtttcaag
    13081 aatgtcaacg attccttcgg gcaccgtgcc ggcgacatcc tgttgcagac cgtagcggcg
    13141 cggctgcgcg gctgcgtgcg cgaaactgat acggtgtgcc ggcacgccgg cgacgagtac
    13201 ctcatcgtcc tttcggcatt gcgcgaaccg gccgaagcgg cgctggtcgc cggcaaggtg
    13261 ctctccacct tcgaggaacc gttcctgctc gacacgcagg aaatccagat ctcggcaagc
    13321 gtcggcatca gcgtctatcc cgatgacggg caaaccatgg aggatctgat ccgcaacgcg
    13381 gatgcggcaa tgtactactc gaagaagagc ggccgcaacc gctacgaatt cttcacgcct
    13441 gaactgactg caccggtggc cgagcgcctg gtgctggcca atcagctgcg ccgcgccatc
    13501 gaacgcaatc aactggtcct tcactatcag ccgcagttcg acaccgccaa tggcaagttg
    13561 ctgggtgccg aggcgctggt gcgctggacc cacgacgagc acggactgct gtttccggag
    13621 agtttcattc ccctggcgga agaatcggac atcatccatc tcatcggcga atgggtattg
    13681 aacgaggcct gccgccagat caacgcctgg cagacggacg gactgcgggt ggtccctgtg
    13741 gccgtcaact attctgcgtt ccagttccgc cgcgccaacc tggtccaggg cgtggcggcg
    13801 gcatttgccc gccataacct caagccgagc cagctcgaga tcgaactcac cgagaacgcg
    13861 atcatgcagg atcccaagga aaccgcgagg accctgcaga tgctgcatga gatgggggtg
    13921 agcctttcca tcgacgattt cggcactggc tactcgagct tgaattacct gaagcgcttc
    13981 ccgatcgaca aactgaagat cgaccgcagc ttcgtcgagg acctgccgca cgacctgaac
    14041 gattccgcga tcgtgcaggc aatcatcaac ctggcgaaga gcctgcgcat gacggtggtc
    14101 gccgaaggcg tggaaacccg gggccagctc gattttctgc gtgcgcttgg ctgcgaggct
    14161 taccagggct acttcggcgg ccggccgatg gaagccgaac gcttcgcggc catgctcgcg
    14221 tgaacgcact caggcggaga tccgtcgcgt gatgtagtcc tctacggcag cgcgttcggc
    14281 ggcgctcagg tgcaggccga tcttgctgcg ccgccacagc acgtcttccg cagtgcgcgc
    14341 ccattccctg tcgataaaat gatcgacttc gcgcgccgtc aatccagcgc cgaaatgtgt
    14401 ccccatgtcg ctgacgttct gtgcgtcgcc gatcaagtcg attgcagtcg atccgtgccg
    14461 ccgcacgata ccttcgacca gcccggtagg caacgcggcg cagcgagtca gcatgctctt
    14521 cagaaactcg acgtaatcga agtggccggt cgcggcaccg ggaagcggcg cagttgcggt
    14581 ccacggacgg cccggaagct ccagcaccga cgaaagttta tccagcgcgt gctcggcaag
    14641 gcgacggtat gtcgtgatct tgcccccgaa tacggacagg atgggcgcgc cgctggcgga
    14701 gtgatcgagc tgcaggacat agtcgcgcgt gacctcggta gagctcgcct tgccatcgtc
    14761 gtgtagcgcc cggactccgg cgtaacacca caccacgtcg ccagcgttta ttggcgtcct
    14821 cagatagcgg ttcgccgcgt cgcacagata gcgcacttct tcatccgaca cctccatgac
    14881 cgcgggcggc tgcgcgagtg ccacctcggt agtgccgatc agggtgaatc tgcgttcgaa
    14941 cggcagaaga aatacaatcc ggcggtcgcg gttctgcaaa atataggcat ggtcgcccgc
    15001 gatcctgcgc ggcacgacga tatggctgcc cttgaccagc cgcactcgcg cctgcacttg
    15061 caggccggcg atttcgcgca acagttgttc gacccagggt ccggcggcgt tgacgatcgc
    15121 tttggccgac aggaatccgc gccgtccatc ggcagcctcg aggtcggcgc gccagccttt
    15181 tcccgtggcg gtgagaccca cgcaggcggt gcgcggcaga attttcgccc cgagagtttg
    15241 cgcagagagc gcattgacca cgaccagacg cgcatcgtcc acccggcaat ccgagtagga
    15301 gaaaccgctg ccgaagcggg ggtcgagatc gtcttcgaag ggcggcgccc gcaaatcgac
    15361 cgcacggctt cgtggcaaag tgctggcgcc gccaagtcgg tcatagagcc acaagccggc
    15421 acggatcatc cacttcggcc gcaggccggg aacgtgagga agcacgaagc gtattggcct
    15481 aaccagatgc ggcgcgagcc gcatcagcac ctcgcgttcc gccagcgatt cgcgtaccag
    15541 acgccactgc cgctgctcca gatagcgcag gccgccgtga atcaatttgc tgctcgccat
    15601 ggaagtcgcg ccggcgagat cgccgcgttc gcacagggcg acgcgaacgc ccctgccggc
    15661 ggcatcgcgg gccagaccca ccccgttgat tccgccgccg atcaccagaa gatcgtattc
    15721 gctgtccagc gtatccatcg cttcgatcgc ccccggggaa tgcagcgtgt cagcgcagcc
    15781 cagccaagac ttcgctcagc gccgagcagg gaggcgttgg cagcattgac gatcgcctcc
    15841 acctgctggc gggtgatgtc gccctgcaac acctgtatgc gccggctcat gggacaagaa
    15901 taacctgttc ccggcgctat catgagcgcc atcaactgct tcataatttg cgggtatgct
    15961 gcagacaagc cgaaccgacg tgaacgccgc gatcacaaac attctggtag tagacgacga
    16021 ccaacgcctg cgcgatctcc tgaaccgcta tctcgccgag caaggattcg gcgtgcacgc
    16081 cgttgccaac ggcagcgcga tggaacgggt gatcagcaag gaaaacgtgg acatgatcat
    16141 cctcgacctg atgctaccgg gcgaagacgg catgagcatc tgccggcggc tgcgcgccgg
    16201 cggaaggcgc acaccgatca tcatgctcac ggcgaaaggg gacgaagcgg accgcatcgc
    16261 cggcctggac gtcggcgcag acgactatct gccgaaaccg ttcaacccgc gcgaactggt
    16321 cgcaaggatc aacgcagtct tgcgccgcca gtcacctgca tccgccctgc ccggcgctcc
    16381 cgatgacagc aaggcgccgg tgcgattcgg ctcattcgaa atcaattttg ccacccgcac
    16441 gctcacccgc aacggcaagc acgtcggatt gacgaccggc gaattcgcca tgctcaaggc
    16501 gctggtgacg cattcgcgca cgccgctatc gcgcgaccgc ttgatggaat tggcgcgcgg
    16561 acgcgagtac ggggtcttcg accgcagcat cgatgtacaa gtctcgcgcc tgcggcgagt
    16621 gctggaggaa gatccggcca acccccgcta catccagacg gtatggggcg tgggctacgt
    16681 tttcgttccc gacgattccc gttaggcttc cccgtcgtgt catgaactgg attccgcaat
    16741 cgctgctgtc ccgcacggta ctggtgatct cggcgctgct ggtggtcagc cagttgatct
    16801 ggctgggctt gtatcggtcc tacaacgtgc gcatccagtc cgaatacctc gccaaccaga
    16861 ttgccagcgt gatcggcacc gtgagcgtgg cgctggaaac catgccgccc gcggtccgcc
    16921 gcaagttcag cgaaagactt ccggcgcacc acaacatcaa gctgttgccg gccaccgcct
    16981 gggacgatga ggacgtaccg ctgccgcagt ccccgctgct gcgcgccatc ggcgaggcat
    17041 tgaggcgcga agtggccgac gacgcgcaag cgctggtgct gctggaggac gccacgcgcg
    17101 ccctgtggat caagatcaaa gtcaagaatc aggcctactg ggtggtattc ccgcccgagt
    17161 cgcgcggttc cgcgtcggcc tgggcctgga ccggttggag cgtattcggg ctgacactgg
    17221 caatcgccgg ggggctgttg ctggtgctgc gggtaaaccg gccgttacgg gcgctggcca
    17281 gcgccgcaga acagatcggt gccggcagga cgcctcctcc gctcgatgaa caaggcccca
    17341 cggaaattcg cacgctgtcc cacgcattca atcagatgac ttccaacctg aagcaactgg
    17401 acaacgatcg cgcggtcctg ctcgctggaa tttcccacga tttgcgcaca ccgctgtcca
    17461 ggttgcggct cggtatcgag atgctcggcg aggccggcga ccatggattg aagaacagta
    17521 tgatccagga tatcgaggat atggacgcga tcgtcaacca gttcctcgac tttgcccgtg
    17581 agggcggaaa cgaaccgtcg cgaaacggcg aggatctcaa ccacatcgtc tccagcgtgt
    17641 gcgagcgatt ctcgcgcatg ggtaaatcgg tgaacaccaa tctgggcgag ctgcctccgc
    17701 ttacgctgaa gccgaccgct acccagagga tggtgaccaa tctggtggac aacgcgctgc
    17761 gttacggcgg tggcgaggtc gaagtcagga ctgagtgcgt cgacggacat gcgctggtgc
    17821 gggtgctcga tcgcggcccc ggcattccgg aatcggaagt ctggcgcgtg atgcaacctt
    17881 ttacccgact ggaggcctcg cgtagcggag gcaacggctc cggacttgga ctggcgatcg
    17941 tcgagcgcgc cgcccgcatg aacggtggca acgtcaagct gctgcgacgt gagggtggcg
    18001 gcctggaagc ccgggtcgaa ctgcccaccg atacccagaa ggcggcatag atctcaataa
    18061 catccttcac cacggagaca cagaggcacg gagaaatgcc ggggattcaa agttccatca
    18121 atgcaattgg cgcgacgtgc acaaatggga tgattgaatt tgttttcaca tcaccacgtt
    18181 ttcgtttcga atggtttaga ttccgggatt ctcttgcctc tgcggttcaa gagtgaacgc
    18241 cagccaaagg ccagcggccg atggtttcgt agcgtgaacc gtcgggcgcg agcgtcgatc
    18301 tcaccagaac aaagtcttcc acttgccagt caataatcgg cgtcgaatcg ggcaagggcg
    18361 cacactctgc cttcctgacc agcgttacgt gcggattgaa gggccgagca tccatctcga
    18421 atcccgcgtc acgcaaccag gcttccattc gcgcggccag ttcgcgcagc gggtcgggaa
    18481 cgcgcgacgg cgctacccag gcgatgccgt tgcgcggcca gcaaccccaa cgatcgagcg
    18541 tcagcgtgaa tcgggtaaca gtcagctcgg ccggcggcac cagcaattca gcgagccgtg
    18601 cgccgtcaac gtctccgaca aaagccatcg tgagatgaat gctgtcatcg cgggtgcgtc
    18661 tgccgccgac ggtgcgatgc atgcgtcgcg ccgtttcggc aagatcgctg ttgacgttcc
    18721 catccggcca gagcgcgtaa aaaacgcgca ggttatgcag attcaacggt ggccgatttc
    18781 caccggcggc cgcgactcga tcaatgcaat cgcctccgcg gcaatgccct caccgcgacc
    18841 ggcaaagcca agcttttcgg tggtctttgc cttcacgttc acgcgctccc tggcgatggc
    18901 cagatcggcc gcgatgttgg cgatcattgc aggtatatgc ggggccatct tcggtgcctg
    18961 ggcgacgatg gtggtgtcca cattgagcac gtggaagtga ttcttctcca gcagggcggc
    19021 cacctcacgc aacagccaac ggctgtccgc accacgatat gccggatcgg tatcgggaaa
    19081 atgacggccg atgtcgccga gcgccgcggc gcccagcagc gcgtcgcaga gcgcgtgcaa
    19141 taacacgtcc gcgtcggagt gtcccgccaa tcctttctcg taggggatgg caactccgcc
    19201 aaggatcagc gggcgtcccg ccaccagcgc gtgcacatcg aatccctgcc ctattcgcat
    19261 tctagtattc ctttaccaca gagacacaga ggcactgaga aaaccggaaa gcatgtcgtt
    19321 tcacaaacac aacgttgatc tgcggtcagg attgaacttc ccgttgtgta cttcgcgtca
    19381 gctacattga ctgcacttcg agcccttcgc attctccgtg cctctgtgtc tctgtggttc
    19441 acatgtgctt gaggatcagt tcggcgagcg ccagatcctc cggataagtc accttgaggt
    19501 tggtgatgtc ggaagcaaca agacgcggtt gcattcccag cctttcgatc gccgaagcct
    19561 catcggtgat gtccgcaagg cccgcgcact gaagggcacg ggtcaacagg tcatagcgaa
    19621 acatctgggg ggtctgagcc tgccataggc cggcgcgcgc ctcggtggcc actacgcgcg
    19681 caatagaatt tgcgcgcttc agcgtatcgg caagcggcac agcgagcaag ccgccagttt
    19741 gatccgcccc gatttcgccg atcaagcggt caagcaatgc cggcgtgacg cacggacgcg
    19801 cagcatcgtg caccagaatc cagtcgtcac ccgacacctc cctttcgatc cgttgcaggc
    19861 cattgcgtac cgtctccgcg cgtgattctc cgccggcgta tatcggcatc agcttcgcgc
    19921 cgagcggatc ccagtcgtat tcgttgaacc atcgatccgc ggctgccagc acgacgtaca
    19981 cacgctccac cagcgcgtgt gcgcacaggc acgagatcga gtagtgaagg agcggtcgat
    20041 tcagtatcgg caggtattgc ttgggcaggg gctggcccat gcggctgccg gcgccggccg
    20101 ccgggatgag ggcgaggtat cgggacatag gcgaaaggtc agcattatca accggcaccg
    20161 ctggccgggc aacaaaactt gcgtcctttc aagttgttga ctgtgaaccg gggtattcga
    20221 tagtcttggc tcttgacttg taaatagaac tcactatcat ttgcattact ttattacatt
    20281 aggactggta caaaaacgtg accctgcgat atcaccttgt tttggcgtta ctcgctgctt
    20341 gcgcattggg tgccgctccc gcatctgccc aggaaaccta caccatcgtg atcaaggatc
    20401 accgcatgaa ccccgccgaa ctggaggttc ccgctgggca gaaaataaag ctgctggtcg
    20461 acaaccagga ccccaccccc gaagagttcg agagtcattc tctgaatcgg gaaaaagtca
    20521 tcccgggcaa ctcgaaggcc gccattttca tcggtccgct caagccggga agctacgagt
    20581 tctttggcga attccaccag gcgacggccc agggccgcat cgtcgcaaaa taggccgggg
    20641 ccacaatgtt cgccaccgca ctgatcgtct tccgggaagt cctcgaggct gctttgatca
    20701 tcggcatcgt gttggcggcc tgccgggcca taccgcgcag gggcctgatg gtatccggcg
    20761 gcatcacggc cggacttgcg ggcgccgtcg ttgttgccgc ggcggcaggc acgattgccg
    20821 aagccgcgtc cggaatgggg caggaaatcc tcaatgcctg catcctcggt gccgcggtgc
    20881 tcatgctcgg atggcacaac gtctggatga gccgccacgg ccgtgaaatt gcccggcaca
    20941 tgggatccgt cggcaggaat attgctcacg gcgccgcgcc ggtgtggatg ctcggcaccg
    21001 taatcgcgct tgcggtgctg cgcgagggat ccgaagtcgt gctgtttctg tatggcgttg
    21061 ccgccggggg agcggattcc gcacagatgc tcgccggcgg cgcggcaggc ctggcaaccg
    21121 gtgccatggt cggctcactc gtttacttcg gtttgcttgg catcccgttg cgttatttct
    21181 tcgcggtgac cagcgtgatg atcctgctgg tagcgtcggg actggcctcg caggcggccg
    21241 gaatgctgct gcaggccggc cttctcccgc cgctgggaga gccgctgtgg gacacctctt
    21301 ccctggtcgc ggacggctcc ataccgggcc ggatgctgca ggcattgatc ggctacagcg
    21361 cgcgtcctgc cggaattcag gttctttcat acgtcgtcac acttttcgct atcgggggat
    21421 tgatgaagtt tatcggccgc atgtcttcca agaccggatc tcacgcggtt accgcactcg
    21481 gcacagcagc cattgctgcc atgttggcgg tctccatgcc ggaaagctcc gaagctgcac
    21541 ctttcaaggt ttactcgccg aacgtcgtca agggagagaa cgagatcgag tatcgcggct
    21601 tccgtgattt cgacggcgat tcctcgcgcg acggcgcgga gatgcacaaa ctcggcgtgg
    21661 ggcgaggctt caccgatttc tggttcagcg agatctacgg cgtttacggg aaggaaccag
    21721 gcggctccta caagcatgaa gcgatcgaat gggaaaaccg tttccagttg accgagcagg
    21781 gaaagtactg ggccgactac gggctgctgg tcgagtacga agctaccgcg cacggcaacc
    21841 cggaccaact cgtcatcgca ccgatcatcg agaagaccgt cgatcgctgg gttggaacgg
    21901 tgaatctttt ttttgaacgc gaagtcggca gcggcaggtc ttcggatacc acgtttgcct
    21961 atgccgccag gttgaagtac cttctgcatc cgcgattcga gccggcaatc gaagcgttcg
    22021 gcgagccggg tcgctttaac cacgccggcg gattcaacgg ccaggaacac tgggccggcc
    22081 cggccgttta cggcgctgtc ggactgggcg ggactcacaa actcgtctac agcgcggcct
    22141 atctgttcgg cgagacctcg gtgtcgagcg acaaccgcgc aatactgcgg ctcgagtacg
    22201 agttctttta ggtcggctgc gcgtttcggc ggctcgttgg gccgccgcga tcctctataa
    22261 tcgcggctac tcttcaggcc gcaacgatgc ttctgcctcc gttgatcccg accccggggt
    22321 cgcgcgccac ctttcctccg ctgcacggat ccgcagacgc cttggcgctc gccgagctgg
    22381 cacgcagcgc gcgtccgctg gcgatcatca ccgagaccgc gcaggatgcg gagcggctgc
    22441 gtgaggaaat cgcctttttc gacccgacgc tgcgcgtatt cctgctgccg gattgggaaa
    22501 cgctgcccta cgacagtttc tcgccgcacc aggacctgat ttcggagcgc ctggccacgc
    22561 tctaccagat cactcacgat gccttcgacg tcgccgtggt gccgatcacc accgcgcttt
    22621 accgcctgcc accggcggcc tatctcgcgg cttacacgtt tttcctcaag cagggcgata
    22681 aactcgatgc gaactcgatg cgcaggcagt tgactctggc cggctactcg cacgtcaccc
    22741 aggtggtctc gcccggagaa ttcagttttc gcggcggcgt catcgatctg ttcccgatgg
    22801 gcagcccatt gccctaccgc atcgacctgt tcgacgacga gatcgagagc atccgctcct
    22861 tcgacgtgga tacccagcgc agcatctaca aggtcaagga ggtgcggctg ctgccggcgc
    22921 gcgagttccc aacggacgac aatgcccgca ccacttttcg ccagcgcttc cgggaaaagt
    22981 tcgaaggcga tccgtccaag agccgcctgt acaaggacat gagccagggt ttcgtccccg
    23041 cgggaatcga gtattacctg ccgttgttct tcgagtccac ggcaacgctc gctgattacc
    23101 ttcctgccgg aacgctggtg tccctgcatc agaacgtggc aaaggcgata agccagttct
    23161 ggcgggacac ccagtcgcgc tacaacctgc tgcgcggaga cccggatcgc ccgctgctgc
    23221 cgcctgccga gttgtttctg cgcgaggacg aatttttcgg cagcctcaag ccgttccccc
    23281 ggatcgaaac cgcttcggct gcgaacagtg agagcgctgc agcacctgcc ccgacgcagg
    23341 cgcttccgcc ggtggcagtc gaccgccgcg ctgacaatcc gctcgccgcc ttgtctgcgt
    23401 ttcacggcaa ctacggcggc cgtgtcctgc tgatcgcgga aagcatggga cggcgggaga
    23461 cactgctcgg gctgctgaac gagtaccagg tacggccgaa agtctgcgac ggctggcagg
    23521 ccttcaaaga tgcgtccgag cggatgatgc tcactgtagg tccgattcat gccggcttcg
    23581 tcgattccgg tgacgccgtt gcactgatca ccgaaaacga gctttatccg tcgcagattc
    23641 gcacacgggt ttcccgggag cgccggattg ccactgaggg catgctgcgc gacctgtcgg
    23701 aagtcaagat cggcgatcca gtggtacacg cccaacacgg catcggccgc tatctcggac
    23761 tcgccaacat gaacctcggc gacggtgaga ccgagttcct tacactcgag tacgacggcg
    23821 gcgacaagct gtacgttcca gtgtcgcaac ttgcggtgat cggccgttac agcggcgcgg
    23881 cacccgaagc tgcgccgctg cacaagctcg gcagcggcca gtgggagaag gcgcggcggc
    23941 gcgcggcggc tcaggtgcga gataccgcgg cggaactgct gaacctctac gccttgcgcg
    24001 cagcacgcct gggctacgct ttcgagctca agccccacga ctacgaggcg ttccgggaag
    24061 gcttcccctt cgaggaaacg ccagaccagg cggcggccat ccaggccgtg atcggcgaca
    24121 tgggggctgg caagccgatg gaccggctgg tgtgcggcga tgtgggcttc ggcaaaaccg
    24181 aagtcgccct gcgagctgct ttcgtcgccg tcgcggacag caagcaggtg gccgttctgg
    24241 tgcccaccac gctgctcgcc gaacagcatt tccagacttt ctccgaccgc tttgccgact
    24301 ggccggtgaa gatcgccgag ctgtcgcgct tccgctcggc gaaggagacc agcggcgcac
    24361 tcgaaggcct ggagcaaggc cgcgtcgata tcgccatcgg cacccacaag ctgctctccc
    24421 gcggcgtgaa gttcaaggat ctcggcctgg tgatcatcga cgaggagcac cgcttcggcg
    24481 tgcgccagaa ggaaaaactg aaggcattgc gcgcccaggt ggacgtgttg acgctcaccg
    24541 ccacgccgat cccgcgcaca ctggcaatgg cgctcgaagg actgcgcgat ttctcggtca
    24601 tcgccaccgc tccacagcgg cgcctggcga tcaagacctt tgtttcaccc ctgagcgagg
    24661 gcctgatccg cgaagccgtg ctgcgtgaac tgaagcgcgg cgggcaggtt tacttcctgc
    24721 acaacgagat cgagaccatc cgcaacatgg aggcacgggt ggcgaagctg ctgccggaag
    24781 cccgcattcg caccgctcac ggccagatgc ccgagcgcga attggaactg gtcatgcgcg
    24841 acttctacca gggccgcttc aacctgctgc tgtgcacgac tatcatcgag accggcatcg
    24901 atgtgccgac cgccaacacc atcatcatca atcgcgccga caagttcggt cttgcgcaat
    24961 tgcaccagtt gcgcggccgc gtcggccgct cgcaccacca ggcatatgcc tatctgctga
    25021 cgcccgatga agacgccatc ggcagccagg cgagaaaacg cctggaagcg atccagatga
    25081 tggaagagct ggggtcgggc ttttacctgg cgatgcacga cctcgaaatt cgcggcgccg
    25141 gggaagtgct gggtgagtcg cagagcggcg aaatgcagga agtgggattc gcgctatatg
    25201 ccgagatgct caacgcggcc gtgaagtcgc tcaaggccgg caaggagccg gagttgtccg
    25261 ggaatttcaa ccccggcacc gagatcaacc tgcacgcgcc cgcgctgctg cccgaggact
    25321 actgtgcaga catccacgag cgcctggtgc tgtacaagcg gctcgccaac tgcgagacgc
    25381 cggaagaact cgaagccatg cacgaagaac tggtggaccg cttcggctta ctgcccgacc
    25441 cggccaaagt cctgctggag acccaccggt tgcgcatcac cgccaggcag cttggcatct
    25501 cccgtatcga cgctggcccg gagagtcttc tgctccaatt cattcccaat ccgccgctgg
    25561 accccgccaa gatcatagga ctggtacaga gccgcaagaa cttcaagttg agcgggcagg
    25621 atcggcttcg ggtggaagct gcaatgcctg acattgccgc ccgcgtgtcg caggcacgcg
    25681 ctgttctcaa agatctcggc tgactttggg gtcgtccgca gccgcggtga cacggctgga
    25741 cttctcagtc ccagcgattg tcgcggacgt agactttccc gtcttcgagc ttcactggaa
    25801 acgtggcagt gggttcgtac gccggtggcg agagc
//
LOCUS       DASQOP010000231         5249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2725658, whole genome shotgun sequence.
ACCESSION   DASQOP010000231 DASQOP010000000
VERSION     DASQOP010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2725658"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..460
                     /locus_tag="VE030_09340"
     CDS             38..460
                     /locus_tag="VE030_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014900079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HZF23655.1"
                     /translation="MKALKGSKTEKNLKDAFAGESQANRRYLYFAAKADVEGQNDVAA
                     VFRSTAEGETGHAHGHLEYLEAVGDPATGLPIGKSRDNLKAAIAGETHEYTDMYPGMA
                     KGARDEGFDEIADWFETLAKAERSHANRFQKALDTLAD"
     gene            565..1908
                     /locus_tag="VE030_09345"
     CDS             565..1908
                     /locus_tag="VE030_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HZF23656.1"
                     /translation="MTVREGSLEAPTRHPIAWKSTDFYDEDKVFDEMHRVFDICHGCR
                     RCVNLCTTFPTLFDLIDEGKTGELDAVAKPDLWKVVDKCYLCDMCYMTKCPYVPPHPW
                     NVDFPHLMLRAKSVKFKKGETRFRDKLLTSTDAVGKLASIPVVVQVVNAVNKAKAARK
                     LMGSVLGVHKDRVLPEYSSARFRSHAKPDSSFPVRGGKNTPGKVAIFATCYVNYNEPG
                     IGHDLLSILFHNEVPAIIVEKEACCGMPKLELGDLDTVQKLKETNIPVLARLAKQGYA
                     ILAAVPSCTLMFKQELPLMFPDDADVKAVQEAMFDPFEYLVLRHKDGLLKTDFKKPLG
                     KVSYHVPCHLRVQNMGQKTREMFGLVPQTQVNTVERCAGHDGTWGVKKEYFDESMKIG
                     KPVFRQMAATEPDYVSSDCAIAGRHIKQGIADSGLDRGNAEKSHPLTLIRIAYGL"
     gene            1950..2537
                     /locus_tag="VE030_09350"
     CDS             1950..2537
                     /locus_tag="VE030_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HZF23657.1"
                     /translation="MAVITAESLMSLEQYAKARKEFRAKVLAHKKNRTVRPGEHVTLV
                     FEDELTMRYQIQEMLRVERIFEEEGIRDELDAYNPLVPDGSNWKATMLIEYPNVEERK
                     LALARLIGIEDRVWIGIAGHPRVYAIADEDLERENEEKTSSVHFLRFELAPPMKRALK
                     DGAGLSVGVDHPNYRASVDAVAAGVRSSLLNDLVF"
     gene            2626..3843
                     /locus_tag="VE030_09355"
     CDS             2626..3843
                     /locus_tag="VE030_09355"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HZF23658.1"
                     /translation="MLVAPMIGMLEDRLRRLHLPLSLTLWNGRIIQSGDSPELRLTVR
                     SPQALVSLINPSLGKIARSYVEGDLDLEGDIRKSVHIGEQLVAGDAGAYRRRSDIWKW
                     WRHTRPADRKAIQHHYDVGNEFYGLWLDRNRVYSCAYFRKPDDSLDLAQEQKLEHICR
                     KLDLKPGERFLDIGCGWGGLILWATRHYGVKALGITLSHEQHAYVTERIGALGLEGRC
                     EVRLMDYRDVPEDQPYDKIASVGMFEHVGKRNLPAYFGKIYRLLKPGGMVMNHGITTN
                     SLEDAALGSGIGDFVEEYVFPGGELVHISRVLGEMSKQGLEAWDTECLRPHYAKTLWH
                     WVSKLEARQEEARRIVGEKRFRTWRIYMAGSAHAFDRGWISIFQILGTKPPADGSVPY
                     PLTREHIYRDEGA"
     gene            3933..4418
                     /locus_tag="VE030_09360"
     CDS             3933..4418
                     /locus_tag="VE030_09360"
                     /inference="COORDINATES: protein motif:HMM:NF020331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HZF23659.1"
                     /translation="MARQRGMPEDIGGVPEVKEFKEDALNIPAYPDDAALIEFRPRGN
                     SKNRFYIDRNSVSLGSDWVVRYTAVIKSPSGVANVSYEAMRCKTSAYKVYAYGTRNGA
                     WTNAREPKWQDVDRTAASFRYSLWADYLCDGEGVRGRNAKDLIARLKGDPLNNFDLKE
                     R"
     gene            complement(4606..>5249)
                     /gene="proB"
                     /locus_tag="VE030_09365"
     CDS             complement(4606..>5249)
                     /gene="proB"
                     /locus_tag="VE030_09365"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HZF23660.1"
                     /translation="VTNLIEADALVILTDQSGLYNKDPRRYKDAELISEARADDPELE
                     KMAGGVGSHLGSGGMLTKVLAARRAARSGAATIIASGREPDVLVRLAGGEAIGTQLIA
                     ETMTLAARKQWLADHLQVRGKLKLDDGAVRALKVDGKSLLPIGVYELTGEFERGEVVS
                     CLDSQGREIARGLVNYGSAETRRILKAPSHEIEARLGYVDEPELIHRDNLVLL"
BASE COUNT         1087 a         1567 c         1627 g          968 t
ORIGIN      
        1 aaccgcagcc gaatcgaatc actgtacgga ggccaagatg aaagcactga agggttccaa
       61 gaccgagaag aatttgaaag acgcgtttgc cggcgaatcc caggcaaacc gccgttatct
      121 ctatttcgca gcgaaagccg acgtggaagg ccagaacgat gtcgcggcgg ttttccgctc
      181 caccgccgag ggtgaaaccg gtcacgcgca tgggcatctc gagtacctcg aagccgtggg
      241 cgacccggcg accgggctgc cgatcggcaa gtcgcgcgat aacctgaaag ccgcgattgc
      301 cggcgagacc catgagtaca ccgacatgta ccccggtatg gccaagggtg cccgcgacga
      361 aggcttcgac gagatagccg actggttcga aacccttgcc aaggcggagc gctcgcacgc
      421 caaccgtttc cagaaggccc tggacacgct ggcagactga agaggcaagg cggaaggatg
      481 aaggcggagg gcggaaggta agccttcgtc tttttcctcg tttcttcctt catccttcat
      541 ccttcatccc tcatccttgt attcatgacc gttcgcgaag gcagcctgga agcgcccacc
      601 agacatccca tcgcctggaa gtcgacggac ttctacgacg aagacaaggt gttcgatgaa
      661 atgcaccgcg tcttcgacat ctgtcacggg tgccgccgtt gtgtgaacct gtgcaccacg
      721 tttccgacgc ttttcgacct catcgacgaa ggcaagaccg gcgagctcga cgcggtggcg
      781 aagccggatc tgtggaaggt cgtggacaag tgttacctgt gcgacatgtg ctacatgacc
      841 aagtgcccgt acgtgccgcc gcacccctgg aacgtggatt tcccgcacct gatgctgcgc
      901 gccaagtcgg tgaagttcaa aaagggcgag acccggtttc gcgataagct gcttaccagt
      961 acggacgccg tcggcaagct cgcctcgatc ccggtggtgg ttcaggtcgt caacgcggtc
     1021 aacaaggcca aggccgcgcg caagctcatg gggtcggtgc tgggggtgca caaggatcgc
     1081 gtgctgcccg agtacagcag tgcccgattt cgcagccatg cgaaacccga ttcctcgttc
     1141 ccggtcaggg gcggcaagaa taccccgggc aaggtggcga tcttcgcgac ctgttacgtg
     1201 aattacaacg agcccggcat cggccacgat ctgctcagca tcctcttcca caacgaagtg
     1261 ccggccataa tcgtcgagaa ggaagcctgt tgcggaatgc ccaagctcga gctgggtgat
     1321 ctcgacaccg tgcagaagct gaaggaaacc aacataccgg tgctggcgag gctcgccaaa
     1381 cagggctacg ccatccttgc cgcggtgccg tcctgcaccc tgatgttcaa acaggaattg
     1441 ccgctcatgt tcccggacga tgccgacgta aaggcggtgc aggaggccat gttcgatccc
     1501 ttcgaatacc tggttctgcg gcacaaggat ggtctgctga agaccgactt caagaagccg
     1561 ctgggcaagg tgtcgtatca cgttccgtgt cacctgcggg tgcagaacat ggggcagaag
     1621 acgcgcgaga tgttcgggct tgtgccgcag acccaggtca acaccgtcga gcgctgcgcc
     1681 ggacacgacg gcacctgggg cgtgaagaag gagtacttcg acgaatctat gaagatcggc
     1741 aaaccggtgt tccgccagat ggcggccacc gagccggatt acgtcagctc ggattgcgcc
     1801 atcgccggcc gccacatcaa gcaaggcatc gccgacagcg gattggacag gggcaacgcg
     1861 gagaaaagcc accccctgac tttgatccgc atcgcctacg ggttgtgaag gccggaatgt
     1921 tgccggcaga gaccagcaag agatcagaga tggctgtcat taccgccgaa agcctgatgt
     1981 cgctcgaaca gtacgcgaag gcgcgcaagg aattccgcgc caaagtactg gcccacaaga
     2041 agaaccggac ggttcgaccg ggcgagcacg tcacgctggt gttcgaggac gaactcacca
     2101 tgcgctacca gatccaggag atgttgcggg tcgagcgcat attcgaggag gagggtattc
     2161 gcgacgagct cgatgcctac aacccgctgg tccccgacgg cagcaactgg aaggccacca
     2221 tgctgatcga gtaccccaac gtcgaggaac gcaagctcgc gctggcgaga ttgatcggaa
     2281 tcgaagaccg cgtctggatc gggatcgcag gtcatccgcg ggtgtacgcg attgccgacg
     2341 aagacctgga gcgggaaaac gaggaaaaga cttcgtcggt acatttcctg cgcttcgaac
     2401 ttgcgccgcc gatgaagcgg gcgttgaagg atggcgccgg cctttccgtc ggcgtggacc
     2461 atcccaacta cagggcatcg gttgacgccg ttgccgccgg ggtgaggagc tcgttgctca
     2521 acgaccttgt tttttaagtt cggcaacagg acactagcca gcaagtacaa tagcggcagc
     2581 ctttccggct gccgtttttt tgaatcgact gcttcgagga atgccatgct ggtagcgccc
     2641 atgatcggca tgcttgagga ccgcctgaga cgcctccacc tgccgttgag cctcacgctc
     2701 tggaatggca ggatcatcca gtccggcgac agcccggaac tgcgcctgac cgtgcgttca
     2761 ccgcaggcgc tggtgtcgct gatcaatccc agtctgggca agatcgcccg gagctatgtg
     2821 gagggcgatc tcgatctgga aggcgacatt cgcaagtcgg tgcacatcgg cgagcaactg
     2881 gtggccggcg acgcgggggc ctaccgccgg cgctcggaca tctggaagtg gtggcgacat
     2941 acccgtcccg ccgaccgcaa ggcgatccag caccactacg acgtgggcaa cgagttctac
     3001 ggtctctggc tcgacaggaa tcgggtctat tcctgcgcct atttcaggaa acccgatgat
     3061 tcgctcgatc ttgcacagga gcaaaaactc gagcacatct gccgcaagct cgatctgaag
     3121 cccggcgaac gtttcctcga catcggctgc ggctggggcg gactgatcct gtgggcgacc
     3181 cggcattacg gcgtgaaggc gcttggcatc acgctctcgc atgagcagca tgcgtatgtc
     3241 acagagcgca tcggtgcgct gggactcgaa ggccgctgcg aagtgcggct gatggactac
     3301 cgcgatgtgc cggaagacca gccttacgac aagatcgcga gcgtcggcat gttcgagcac
     3361 gtcggcaaac gcaatctgcc ggcgtatttc ggcaagattt accggctgct caagccgggc
     3421 gggatggtca tgaaccacgg catcactaca aattcgcttg aagacgctgc gctgggcagc
     3481 ggcatcggcg atttcgtcga ggaatacgta tttcccggcg gcgagctggt gcatatctcc
     3541 cgggtcctgg gcgaaatgtc gaagcaggga ctggaagcct gggacacgga gtgcctgcgt
     3601 ccgcattatg cgaagacgct ttggcactgg gttagcaaac tggaggcgcg ccaggaagaa
     3661 gcgcgcagga tcgtgggcga aaaacgcttc cgcacctggc gcatctacat ggcgggttcg
     3721 gcgcatgcgt tcgaccgcgg ctggatctcg atcttccaga ttctcgggac caaaccgcct
     3781 gccgacggct cggtgcccta tccgctcacc cgcgagcaca tatacagaga tgaaggcgca
     3841 tgacgcacaa acgatcacat cgtagtggtt cattccggct ggctgcggct gtctttttgc
     3901 tcgcgttggc gttgtacgaa ggcgatggag cgttggcccg gcagcgcggc atgcccgagg
     3961 acatcggcgg ggtgcccgag gtgaaggaat tcaaggaaga cgcgttgaac attcccgcct
     4021 atccggacga cgcggcactg atcgaattcc ggccgagggg gaattcgaag aaccggttct
     4081 acatcgatcg caattccgta tcgctgggat cggactgggt ggtgcgctac accgcggtca
     4141 tcaaatcacc ttccggggtc gccaatgtca gctacgaagc tatgcgctgc aagacgtcgg
     4201 cctacaaagt gtacgcgtac ggcacgcgca acggagcatg gacgaacgct cgcgaaccca
     4261 agtggcagga tgttgaccgc accgcagcca gtttccgcta ctcgctatgg gctgactatc
     4321 tgtgcgacgg cgagggcgtc aggggtcgaa acgcgaaaga cctgattgcc aggctcaaag
     4381 gcgatccgct gaacaacttt gatctgaagg agcggtagac ctacttttgc agaaaggcga
     4441 ggttgtagtt ccggcacact ctgcgagctt aacctgcccg ccacctgcga tcaggttagc
     4501 ctccactgca atcgtgtgct gctagagcag cacagattgt ggcttccacg taacgcggca
     4561 cagccgcatt agtgtccgcc gtaactgatt cctgctctga ggaattcaga gcaggactaa
     4621 gttatctcga tggatcaact cgggttcgtc gacgtagccc agccgtgcct cgatttcgtg
     4681 gctgggagcc ttgaggatgc ggcgggtttc cgccgagccg tagttcacca gcccgcgggc
     4741 gatttccctc ccctgcgaat cgaggcagct caccacctcc ccgcgctcga attcgccagt
     4801 cagttcgtat acgccgatcg gcagcaggct cttgccgtcc accttcagcg cccgcaccgc
     4861 gccgtcgtcg agcttgagct tgccgcgtac ctgcagatgg tcggccagcc actgcttgcg
     4921 ggcggcgagc gtcatggtct cggcgatcaa ttgggtaccg atggcttcgc ctccggcgag
     4981 ccgcaccaga acatccggct cccgtcccga agcgatgatg gtggcggcgc cgctgcgggc
     5041 cgcgcgccgc gcggcaagca ccttggtgag catgccgccg ctgccgagat ggctgcccac
     5101 gccgcccgcc attttttcca attcgggatc gtccgcccta gcttcgctga tcaattccgc
     5161 gtccttgtag cggcgcggat ccttgttgta cagaccggac tggtcggtga ggatcaccaa
     5221 ggcatcggcc tcgataaggt tggtcacca
//
LOCUS       DASQOP010000232        21306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2926163, whole genome shotgun sequence.
ACCESSION   DASQOP010000232 DASQOP010000000
VERSION     DASQOP010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 21306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21306
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2926163"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..2778
                     /gene="aceE"
                     /locus_tag="VE030_09370"
     CDS             97..2778
                     /gene="aceE"
                     /locus_tag="VE030_09370"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004538122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HZF23661.1"
                     /translation="MAALPDDDPQETNEWLDALHGVLRHEGPERAHYILEKLLEEARR
                     SGVYLPYSANTQYINTIPPGKEERSPGDHELEHRIRSYVRWNAAAMVLRANKQSSELG
                     GHIASFASAATLYDVGFNHFWHAPSENHGGDLVLFQGHSAPGVYARAFMLGRLTREQM
                     DNFRQEVDGRGLSSYPHPWLMPDFWQFPTVSMGLGPIMAIYQARFLKYLQDRGLANTE
                     GRKVWCFLGDGEVDEPESMGAIGVASREKLDNLIFVVNCNLQRLDGPVRGNGKIIQEL
                     ESDFRGAGWNVIKVIWGSYWDPLLAKDRNGLLVKRMEECVDGEYQTFKSKDGAYVRKH
                     FFGKYPELLEMVANMSDDDIWRLNRGGHDPHKIYAAYSAAVKHQGQPTVILAKTIKGY
                     GMGEAGEAQNITHQQKKMGTTSLKAFRDRFNLPVPDDKLEEVPYLTFQEGSAELEYMR
                     KHREALGGYLPMRRLKGDALEIPALSAFEPLLKSSEEREVSTTMAFVRILNILVKDKN
                     IGKRVVPIVPDESRTFGMEGMFRQLGIWSSVGQLYTPQDADQLMFYKEDKNGQILQEG
                     INEPGAMSSWIAAATSYSTHGVSMIPFYIYYSMFGFQRVGDLAWAAGDQRARGFLLGG
                     TSGRTTLNGEGLQHEDGHSHVLSSTIPNCISYDPTFSYEVAVIIQDGLRRMVKEQQDV
                     FFYITLLNENYTHPAMPKGAEDGILKGMYKLREGRGERAEGRGKKDTPRVQLLGSGAI
                     LREVIAAQDLLEKDFGVSADVWSATSFNELRREGLDVQRWNLLNPEQSPREAYVSQCL
                     NHAPGPVVAATDYMKIFADQIRPFIYKPYVVLGTDGFGRSDYRRQLRKYFEVDRYYVT
                     VAALKALADEGSVPASTVAKAIKKYTIDPGKPNPVTV"
     gene            2913..4220
                     /gene="aceF"
                     /locus_tag="VE030_09375"
     CDS             2913..4220
                     /gene="aceF"
                     /locus_tag="VE030_09375"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HZF23662.1"
                     /translation="MGSLRDVLVPDIGDFKNIPVIEVLVKPGDAVKAEDSLITLESDK
                     ATMEVPAPFAGVVKEMKIKPGDKVSEGMPILSLEISDGAPAPAAPHKPAAPAPAAQAT
                     AAVKPAPAPAPASPPSPQPAVGLIDEAGFSKAHASPSVRRFARELGVDLARVKGSGPK
                     ERILKEDITNFVKNALAHPASASGGGAGLALGLPAWPQVDFAKFGPVETQPMSRIKRI
                     SGPALHRNWVHIPHVTQNDEADITELEAFRKSMAEEAQKQGVKLTPLALIMKAVVAAL
                     KIHPEFNSSIAPSGDALILKRYYHIGVAVDTPGGLVVPVIRDVDQKGVLQLAKELGEA
                     SAKARDGKLGPADMQGGSFSISSLGGIGGTHFTPIINAPEVAILGVSKSTTKPVWKDG
                     QFVPRLMLPLSLSYDHRAVDGAQGARFITYLNSVLSDIRRVLL"
     gene            4400..5068
                     /gene="nadD"
                     /locus_tag="VE030_09380"
     CDS             4400..5068
                     /gene="nadD"
                     /locus_tag="VE030_09380"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HZF23663.1"
                     /translation="MSGCAPIGILGGTFDPIHFGHLRLAQELADALGLARVRFIPTGT
                     PPHRNSPQVSGAHRLQMVAVAIAGNPLFEADDREIRREGISYTYDTLTELREELGEGP
                     LCLLMGADAFGALTTWHRWQDLFDLAHVVIAHRPGFRLQELQAALPAPLRKIYLQRLA
                     RVPGMLRANAGSILTREITALDISATHIRAMLARGSSPRYLAPDTVLEYIDHNQLYRD
                     RDAR"
     gene            5058..5444
                     /gene="rsfS"
                     /locus_tag="VE030_09385"
     CDS             5058..5444
                     /gene="rsfS"
                     /locus_tag="VE030_09385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HZF23664.1"
                     /translation="MHVEAIKQAVVEALEEIKAADITVLDVRQMTSLFDYMIIASAGS
                     TRQTKALANNVQDKLRQIGARVLGVEGEQTGEWVLVDLGEIIVHVMQPAVRAYYNLEQ
                     LWGGTPPSPHPTNWRNAPGAEPSRAL"
     gene            5467..5949
                     /gene="rlmH"
                     /locus_tag="VE030_09390"
     CDS             5467..5949
                     /gene="rlmH"
                     /locus_tag="VE030_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HZF23665.1"
                     /translation="MKLHVVAVGRKPPDWLAAGFEEYARRMPRSARIDLIEVKPAPRG
                     RETPSEALVSRVLAEEKSRIFAAIPSGCVKIALDERGKQLSTAELARRLDGWMQGGRN
                     VAFVIGSADGLDRDLKAGADLLLSLSAMTLPHALVRVLLAEQLYRAVSLIQNHPYHRD
                     "
     gene            6001..6621
                     /locus_tag="VE030_09395"
     CDS             6001..6621
                     /locus_tag="VE030_09395"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HZF23666.1"
                     /translation="MPALEKHIYLASRSARRRDLLKQMGVNFEILLLREGAGRNADFN
                     ESELPGETPREYVVRVARLKAEAGWLRLEQRRLMRHPVLAADTTVALGDTILAKPKDR
                     EDAVAMLKQLSGTVHHVYTAMAIKFYDSLQEALSVTEVRFRELSDDEIRRYVATGEPL
                     DKAGAYGIQGKASMFIEAINGSYTGVVGLPVFETARLLGHFGFQLL"
     gene            6618..8072
                     /gene="rng"
                     /locus_tag="VE030_09400"
     CDS             6618..8072
                     /gene="rng"
                     /locus_tag="VE030_09400"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011545847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HZF23667.1"
                     /translation="MNEEILINVTPQETRVAMMQQGVAQELHIERSGSRGLVGNIYLG
                     EVSRVLPGMQSAFIQIGLDRAAFLHVADIWGHRQNHDESKPIERILHDGQKLLVQVIK
                     DPIGTKGARLSTQVSIAGRLLVYLPQESHIGISQRIEDEAERALLREKLQHLILADEP
                     GGFIVRTMAETASEKELGTDIEYLRKLWSDIKEKAAIATAPALLYQDLTLSLRVLRDF
                     VNDRCDRVLVDSRETFQRMQAFANDYTLSFADRIQHYSGDRPLFDLYGVEDEIERALA
                     RRVPLKSGGYLIIDQTEALTTIDVNTGGFVGGRTFDDTIFKTNLEAAQVIARQLRLRN
                     LGGIIILDFIDMDNEVHRDAVLSEFRKALALDRTRMTVNGFTQLGLVEMTRKRTRESL
                     AHILCEPCPTCQGRGEVKTAQTICYEILRELLREARQFDAREFRILASQMVIDMFLDE
                     ESQSLAMLEEFIGKPISLQVETIYTQEQYDIVLM"
     gene            complement(8127..8441)
                     /locus_tag="VE030_09405"
     CDS             complement(8127..8441)
                     /locus_tag="VE030_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HZF23668.1"
                     /translation="MAQDDILTGVLLDEVALNAEDLARACAVESRWVVERVEAGLLGS
                     ASVKTVEWRFVSADLARARRLAALERDFDANQELAALVVDLIEEVGRLRGRLKAAGLA
                     AD"
     gene            complement(8445..9383)
                     /locus_tag="VE030_09410"
     CDS             complement(8445..9383)
                     /locus_tag="VE030_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HZF23669.1"
                     /translation="MEFKDYYQILGVEKTAPADEIRKAYRKLARKYHPDVSKEPDAEK
                     RMKEVNEAYAVLSDPEKRAAYDQLGSRWQPGQDFQAPPGWDAGFEFSGDGAHSGGAAD
                     FSDFFASLFGRAGRDRGAGDFQMRGGDHHARILIGLADAYHGATRTITLRAAKQDASG
                     HVITEERALNVQIPKGVKEGQHIRLAGQGSPGMGGGPAGDLYLEVQFSPDPRFRVEGR
                     DVYESVPVAPWEATLGATIEVPTPSGNVQVKVPAGSQAGRKLRLKGRGIPGNPPGDLY
                     LVLEVVLPPADTDKARQIYQTMARELAFNPRQKLGV"
     gene            complement(9396..11339)
                     /locus_tag="VE030_09415"
     CDS             complement(9396..11339)
                     /locus_tag="VE030_09415"
                     /inference="COORDINATES: protein motif:HMM:NF012570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HZF23670.1"
                     /translation="MKISDLENQVTQYQQWRDELRACIEAYQSWLENHGHADIQRSLR
                     IYDLAESLRNDRMILAFLAEFSRGKTELINAMFFSDFKQRLLPSDIGRTTMCPTEIYY
                     DATEEPYIKLLPIESRKSDESIAALKHQTVEWVKIRLDLSSHEEMTKAMSSLAQVKSV
                     TVEEAGALGLLDESDFVTTTVVVKHGGRVEIPCWRHALVNYPHPLLKKGLVILDTPGL
                     NALGTEPELTLSMIPNAHAVLFLLAMDTGVTKSDLDVWQKYVQSYVTRRIAVLNKIDL
                     MWDDLKSEDQIEVGIGRQVDETAKILELPRDHVIALSAQKGLIARIRDDDELLRRSRI
                     GDLERLLAEEIIPSKQQILRAAVSREIGGMVETSLQSVTAQLASAKSELQEMSKLSGK
                     NRDLAKLLLAKLEADRANYLRHMESFKHSYGTVLKQGQVLLDTLSDERLDDIMRKSQQ
                     TIEGSWTTAGLMRSMQALFDTFSRQAEKILNFAGETRNYVDGVYNEFHKMYGFRKMTP
                     PALNLERHILRMHSLQQSTERFCKDPVNVMTEKHFLVRRFYNGLVAHARDLFRALRVE
                     LDSWIRSALLPLSMQLKEHQKLLERRVENLRKIAGDINTLQERVRQLEQQQLQLGKQV
                     EELTRIRDVLAREPATAKVAKVA"
     gene            complement(11336..11641)
                     /locus_tag="VE030_09420"
     CDS             complement(11336..11641)
                     /locus_tag="VE030_09420"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HZF23671.1"
                     /translation="MAESWYSYDPDRDLLRFIVRVQPNASRTEFAGFHGGYPKIRVAG
                     PAIEDRANALLLDFLKKKFDLPGGRVIITRGSRGRTKTIEISNPGQTLLVRLKELLQ"
     gene            complement(11645..12217)
                     /locus_tag="VE030_09425"
     CDS             complement(11645..12217)
                     /locus_tag="VE030_09425"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HZF23672.1"
                     /translation="MNQALVFLLETFLGLFALALLLRFYLQAVRAPARNPLSQFLAAL
                     TDFMVRPARRVIPGLWGYDMSTLLLAWAVEVLLIVSIRALSGFEMAPAVGAAIVGLIL
                     LAVVNLLKRFLYILMVAVIAQAILSWVNPHSPAMSMLVSLTRPFLRPFQRRIPPVGGV
                     DLSPLFVLVLCQLLLMWPVAPLIITFSRML"
     gene            complement(12236..13048)
                     /gene="proC"
                     /locus_tag="VE030_09430"
     CDS             complement(12236..13048)
                     /gene="proC"
                     /locus_tag="VE030_09430"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HZF23673.1"
                     /translation="MRILFIGGGNMASALIGGLLKQGLSASDIRVVEVSEDARHRLRQ
                     ALGVETQAGLDEAGVVEDVVLLAVKPQHLRDVAQWLRPHLHGQLVVSIAAGIRTADLS
                     RWLGGHGRIVRAMPNTPALMRAGITGLYAAPKATEADRKTAQRVLGAVGTVIWLDDEG
                     RMDGVTAVSGSGPAYVFYFIEALQQAAQELGFSEEQARSLAVETFVGAAKLAAGSTDP
                     PAVLRAHVTSKGGTTERALEVMESCELKGRVVDAVRAAARRSGELGDEYGKI"
     gene            complement(13164..13988)
                     /locus_tag="VE030_09435"
     CDS             complement(13164..13988)
                     /locus_tag="VE030_09435"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HZF23674.1"
                     /translation="MTTIASRLQAVKARISEAARVAGRAPGEITLVAVSKTFPPKAIA
                     EAYAAGQQAFGENYAQEGIEKIAALFDLLRQRNASPERPLLWHVASPNQDDERTPGDV
                     RPSAGVTSPEGPLLRPMVWHHIGPIQSNKTRAIAENFDWVHGIDRAKIAERLSVSRPD
                     DRPPLQVCIQVNVSGEASKSGVVPDAVFGLAQAIMRLPGLRLRGLMAIPEPTADTALL
                     RSRFRMLREIRDDFLRRGVALDTLSMGMSDDLEAAIAEGATVVRVGRAIFGERGAG"
     gene            14103..15143
                     /locus_tag="VE030_09440"
     CDS             14103..15143
                     /locus_tag="VE030_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HZF23675.1"
                     /translation="MDISELLAFVVKNKASDLHLSAGLPPMIRVHGDVRRINLPPMEH
                     KDVHAMIYDIMNDGQRKFYEENLECDFSFAIPNLARFRVNAFVQNRGAGAVMRTIPSK
                     VLTLEELKAPNIFKEIAQYPRGVVLVTGPTGSGKSTTLAAMVNDINENEFGHILTVED
                     PIEFVHESKKCLINQREVGPHTLSFNNALRSALREDPDIILVGELRDLETIRLALTAA
                     ETGHLVFGTLHTSSAAKTIDRIIDVFPAAEKEMVRAMLSESLRAVISQTLCKTKDGGG
                     RVAAHEIMIGTPAIRNLIREAKVAQMYSAIQTGQNLGMQTLDQNLSDMVKKNLITKEE
                     ARGKAASKDAFL"
     gene            15191..16327
                     /locus_tag="VE030_09445"
     CDS             15191..16327
                     /locus_tag="VE030_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HZF23676.1"
                     /translation="MEKDQAVRFIHELLRGMVARKASDLFITAGFPPAFKVDGKMTPV
                     SNQSLTPQHTQELARSLMNDKQAQEFERTKECNFAINPPGIGRFRVNAFVQQQRVGIV
                     LRTITTTIPKFEDLGLPPVLKDVAMTKRGLVIFVGGTGSGKSTSLAAMIGYRNENSYG
                     HIITIEDPIEYVHDHKNCLITQREVGVDTDSWEAALKNTLRQAPDVILIGEIRERETM
                     EHAIAFSETGHLCLGTMHANSANQALDRIINFFPEERRAQLLMDLSLNLKGLISQRLI
                     PLKDTKGRCAAIEILLNSPLISDLIFKGEVHEIKEIMKKSRELGMQTFDQALFELYEI
                     GKISYEDAMRNADSMNELRLQIKLHGKEAKDRDLTTGVDHLNIV"
     gene            complement(16438..17616)
                     /locus_tag="VE030_09450"
     CDS             complement(16438..17616)
                     /locus_tag="VE030_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HZF23677.1"
                     /translation="MKQERFFAPCPRGLEALLAAELGELGAADIGAVDGGVGFKGTLS
                     LAYCANLHSRIASRVLWEIGSGHYRNAEDVYLGVYALDWQRLFDPGLTIRVNVAAVAS
                     PLKSLEFVTLRIKDAACDKFRAQAGRRPSVDTAQPDVRIHAFLDVERFTIYLDTSGEP
                     LFKRGSRQMAGEAPLRENLAAGILKLAGWTPGTPLLDPMCGSGTLLIEAAQIARGIPA
                     GRNRRFAFEKLRSFDAAKWVSIRDEAEACIRNDAGTPIFGSDLFGDALKLARENLAAA
                     GLDDAVNLKQANVLEISAPAAGGVLVTNPPYGVRLGEQEKLAAFYPQLGNVLKKKFAG
                     WRACVFTADLRLPKLIGLSPSKRTPLYNGPLECRLYEFKIVAGAMRREKQRRGARDEG
                     "
     gene            complement(17814..18194)
                     /locus_tag="VE030_09455"
     CDS             complement(17814..18194)
                     /locus_tag="VE030_09455"
                     /inference="COORDINATES: protein motif:HMM:NF020376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HZF23678.1"
                     /translation="MTALVRGLLIAALWCACPLQAVQVTARILVQNSPLAGFQYYQGK
                     ELWGQMKVGDALALIREPDNPHDPLAVRVEWNGHKLGYVPRKENEAVALQLDRGNPLQ
                     ARIVRLTKHRDPWKRIEFEIFLGL"
     gene            complement(18200..18613)
                     /gene="hemJ"
                     /locus_tag="VE030_09460"
     CDS             complement(18200..18613)
                     /gene="hemJ"
                     /locus_tag="VE030_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HZF23679.1"
                     /translation="MLWIKSFHIIFMVTWFAGLFYLPRLFVYHAMSEDRPSIERFKIM
                     ERKLFFGIMTPGAVLTLLSGAWLWLGYGFHGGWLAAKLALVAVLVAYHLYCGRLMFDF
                     KRDRNRHGHVFYRWLNEFPVVLLVAIVLLVELQPF"
     gene            complement(18623..19408)
                     /locus_tag="VE030_09465"
     CDS             complement(18623..19408)
                     /locus_tag="VE030_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZF23680.1"
                     /translation="MRAKDFRPPVLSLRFAHVWRRNFLVWRKLAIPSMLGNLADPMLY
                     MLGLGYGLGALVGKVGGLPYITFLAAGTVCYSTMNTATFEALYSAFSRMHVQRTWDAI
                     MNAPINLDDVLLAEAVWAASKSFLSGCAILIIIWALGLSHAPLTLWIVPLAFLVGLTF
                     ACLGLVMNAVSPSYDFFLYYFTLLITPMVLLCGVFFPTDQLPGALQAVSAVLPLTHAV
                     QLARPLVVGVAPAEPAFHVLVLLGYAAVAFYVALVLTRRRLFF"
     gene            complement(19477..20388)
                     /locus_tag="VE030_09470"
     CDS             complement(19477..20388)
                     /locus_tag="VE030_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZF23681.1"
                     /translation="MPLLEVKDLRKSYGNLEVVRGVSLSLEAGECHGLLGPNGAGKTT
                     TLRLCLGLADPDAGEISLLGLPVPQAAREARIRLGVLPQLDNLDPDFTVLENLLVYGR
                     YFGQADAVTRARIPELLEFAGLTNRANSKIQTLSGGMKRRLSLARALINDPDLIFLDE
                     PTTGLDPQARHLIWERLKQLLNRGKTILLTTHYMDEAERLCRRLSIMDHGRIISTGSP
                     RELISRHIEPEVVEVYGEDVAGWAAEHGDKLAMRAEHTGETVFCYTTRAEPLIATLQN
                     RPGLRYLHRPANLEDVFLKLTGRDLRD"
     gene            complement(20459..21070)
                     /locus_tag="VE030_09475"
     CDS             complement(20459..21070)
                     /locus_tag="VE030_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZF23682.1"
                     /translation="MKSVTQSRLSIDGPAGRLEIVANDPGEARRGVALVAHPHPLYGG
                     TLDNKVVQTLAKAFFASGYCALRMNFRGVGASEGVFDEGRGEIEDFLVLAEHARRTYG
                     EGELALAGFSFGGFVAASVAGRLEAKHLVLVAPAVGRFPVGTVPAGTLVVHGEEDDVV
                     PLKDALDWARPQQLPIVVFPGTGHFFHGNLVTLQKLVEQHCRA"
     gene            complement(21067..>21306)
                     /locus_tag="VE030_09480"
     CDS             complement(21067..>21306)
                     /locus_tag="VE030_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HZF23683.1"
                     /translation="NHHAQEMRDYAKARVKSLQLAGPGKVRVNQAGCMDRCDEGPVIA
                     IYPQEIWYTYVDKSDIDEIIDEHLVHGRIVERLRI"
BASE COUNT         4221 a         6812 c         6481 g         3792 t
ORIGIN      
        1 ggcttgccgg tgatataatt tttgccaaaa tcgggcgcgg cgtcacgaac accgcagccc
       61 cggataacga cggcaacccc agcaagggca gaaactatgg cagcgcttcc ggacgacgat
      121 ccacaagaga cgaacgaatg gctggatgcc ctccacggtg tgctccggca cgaggggccg
      181 gaacgtgcgc actacatcct cgaaaaactg ctcgaggagg cgcgccgctc gggcgtgtat
      241 ctgccgtaca gcgccaatac ccagtacatc aacaccatcc cgcccggcaa ggaggagcgc
      301 tccccgggag accacgagct ggaacaccgg atccgctcgt acgtgcgctg gaacgccgcc
      361 gcgatggtgt tgcgcgccaa caagcagtcc tcggaattgg gcggccatat cgccagcttc
      421 gcctcggccg ccacgctcta cgatgtcggc ttcaatcact tctggcacgc gccttcggag
      481 aaccacggcg gcgacctggt gttgttccag ggacactctg cgcctggggt ctatgcccgg
      541 gccttcatgc tcgggcggct gaccagggaa cagatggaca acttccgcca ggaagtggac
      601 ggcagaggcc tgtcttccta tccgcacccg tggctgatgc cggacttctg gcagttcccg
      661 accgtatcga tgggtctggg gccgatcatg gcgatctacc aggcgcggtt cctgaagtac
      721 ctgcaggacc gcggtctggc caataccgag ggacgcaagg tctggtgctt cctcggtgat
      781 ggcgaggttg acgaacccga gtcgatgggc gcgatcggcg tggcgtcgcg cgaaaagctc
      841 gacaatctga tcttcgtggt gaactgtaac ttgcagcgcc tggacggccc ggtgcgcggc
      901 aacggcaaga tcattcagga gttggaatcg gatttccgcg gcgccggctg gaacgtgatc
      961 aaggtgatct ggggctcata ctgggatccg ctgctggcca aggacaggaa cggcttgctg
     1021 gtcaagcgca tggaagaatg cgtggacggc gagtaccaga cgttcaagtc caaggacggg
     1081 gcctacgtgc gcaagcactt cttcggcaag tacccggaat tgctggagat ggtggccaac
     1141 atgtccgacg acgacatctg gcggctgaac cgcggcggcc acgatccgca caagatttac
     1201 gccgcgtata gcgctgcggt aaagcaccag ggccagccga cggtgatcct ggccaagacc
     1261 atcaagggtt acgggatggg cgaggccggc gaggcgcaga acatcaccca ccagcagaag
     1321 aagatgggaa cgacctcgct gaaggcgttc cgcgaccgct tcaacctgcc ggtccccgac
     1381 gacaagctgg aggaagtgcc ttacctcact ttccaggaag gttctgccga actggaatac
     1441 atgcgcaagc atcgcgaagc gctgggcggc tacctgccga tgcgccgcct caaaggcgac
     1501 gcgctggaaa taccggcgct gtctgccttc gaaccgctgc tgaagagctc ggaggagcgc
     1561 gaggtttcta ccacgatggc gttcgtgcgc atcctcaaca ttctggtgaa ggacaagaac
     1621 atcggcaagc gagtggtgcc gatcgtgccg gacgaatccc gtactttcgg catggaaggc
     1681 atgttccggc agctcggcat ctggtcttcg gtcggccagc tctatacgcc gcaggacgcc
     1741 gaccagttga tgttctacaa ggaggacaag aacggccaga tcctgcagga aggcatcaac
     1801 gagcccgggg cgatgtcctc gtggatcgcc gcggcgacat cgtattcaac gcacggcgtg
     1861 tcgatgatcc cgttctacat ctactactcg atgttcggct tccagcgcgt cggcgacctg
     1921 gcctgggcgg ctggcgacca gcgtgcgcgc ggctttctgc tgggcgggac ttccggccgc
     1981 accacgctga acggcgaggg cctgcagcac gaggacggcc acagccatgt tctgtcttcg
     2041 accatcccga actgcatttc ctacgacccg accttctcgt acgaagtcgc cgtgatcatc
     2101 caggatggac tgcgccgcat ggtcaaggag cagcaggacg tcttcttcta catcactctg
     2161 ctcaacgaga attacaccca cccggcgatg ccgaagggcg ccgaagacgg tattctcaag
     2221 gggatgtata agctgcgcga ggggagaggg gagagggcag agggcagagg gaaaaaggac
     2281 accccaagag tgcagttgct cggcagcggc gcgattctgc gcgaagtcat cgccgcgcag
     2341 gacctgctgg aaaaggactt cggcgtatcc gccgacgtgt ggagcgcgac cagcttcaac
     2401 gaactgcgcc gcgagggtct cgacgtgcag cgctggaacc tgctgaaccc cgagcaaagt
     2461 ccgcgcgaag cctacgtgag ccagtgcctg aatcacgcgc ccggcccggt cgttgccgcg
     2521 accgattaca tgaagatctt cgccgatcag atccggccgt tcatctacaa gccttacgtg
     2581 gtactcggca ccgacggttt cggccgctcc gattaccggc ggcagttgcg caaatacttc
     2641 gaagtcgatc gctactacgt caccgtggcc gcgctcaaag cgctggcgga tgagggcagc
     2701 gtgccggcct cgacggtggc caaggcgatc aagaagtaca cgatcgatcc gggcaagccg
     2761 aacccggtaa cggtatgaaa atcggttaag gcgtgaaggg tgaatgtgtg aagggtaacg
     2821 gctccaaacc atgcccaaac ccacgcttca ctcatccact aatcccgttc acccttcact
     2881 cattcactca ttcacccttc actgactaaa ccatgggaag cttacgcgac gtcctcgttc
     2941 ccgacatcgg tgatttcaag aacatcccgg tgatcgaagt gctggtcaag cccggcgatg
     3001 ccgtcaaggc ggaagactcg ctgatcactc tcgaatccga caaggcgacg atggaagtcc
     3061 cggcgccttt tgccggcgtg gtcaaggaaa tgaagatcaa gcccggcgac aaggtatcgg
     3121 aaggcatgcc gatcttgagc ctggaaatca gtgacggtgc gccggctccc gcagccccgc
     3181 acaagcctgc cgctccggca ccagcggccc aagctaccgc ggccgtcaaa ccggcgcccg
     3241 cacctgctcc ggcatcaccg ccctcacccc agcctgccgt cgggctgatc gacgaggccg
     3301 gcttctccaa ggctcacgcc agcccttcgg tgcgccgctt cgcacgcgag ttgggcgtcg
     3361 atctcgcccg cgtcaaaggt agcggcccga aggagcgcat cctcaaggaa gacatcacca
     3421 acttcgtgaa gaacgcctta gcgcatccgg ccagcgcatc cggcggggga gccggtctgg
     3481 cattgggact cccggcctgg ccgcaggtgg actttgcgaa gttcggcccg gtcgaaaccc
     3541 agccgatgtc gcggatcaag aggatttccg gtccggccct gcatcgcaac tgggtgcata
     3601 tcccgcacgt gacccagaac gacgaagccg acatcaccga gctcgaagcc ttccgcaaga
     3661 gcatggccga ggaggcgcag aaacagggcg tcaagctgac gccgctcgcg ttgatcatga
     3721 aggccgtagt ggccgcgctc aagatccacc cggagttcaa ttcctcgatc gcgccctccg
     3781 gcgacgcgct gatcctcaaa cgctactacc atatcggggt tgccgtggac acaccgggcg
     3841 gcctggtggt gccggtgatt cgcgacgtgg accagaaggg cgtgctgcaa ctggcgaagg
     3901 aactgggcga agcgagcgcc aaggcgcgcg acggcaaact cggacccgcg gacatgcagg
     3961 gcggcagctt ctcgatatcc agcctcggcg gaatcggcgg cacacacttc acgccgatca
     4021 tcaacgcccc cgaagtcgcc atcctcggcg tatctaaatc gacaaccaaa ccggtgtgga
     4081 aggacggtca attcgtgccg cggctaatgc tgccgctgtc gctgtcctac gatcaccgtg
     4141 cggtcgacgg cgcgcagggg gcaaggttca ttacctacct caacagcgtg ttgtcggaca
     4201 tccgccgggt gctgctgtaa atcaaggatg gagagcaagg atgagggatg aaggcggaag
     4261 gatgaaggat gaaggatgaa ggcggaaggc tagaggcgga cgacaggagc gtggtgtacg
     4321 cagcgcgcac ggcgagttct gcttttactt catccttcat ccttcatccc tcatccttgc
     4381 ctttttgggc acgccgcact tgagcgggtg cgctccaatc ggtattctcg gcggaacctt
     4441 cgaccccatc catttcgggc atctgcggct ggcgcaggaa cttgcggacg cgctcggcct
     4501 ggcacgcgtg cgcttcatcc ccaccggcac cccgccgcac cgaaactcgc cgcaggtgag
     4561 cggcgcgcac cgcctgcaga tggtagccgt cgcgatcgcc ggcaatcccc tgttcgaagc
     4621 ggacgaccgg gaaatccgcc gcgagggtat cagctacacc tacgatacct tgaccgaatt
     4681 gcgcgaagag ctgggagagg gcccgctctg cttgctgatg ggggcggatg cattcggcgc
     4741 gctgaccacc tggcatcgct ggcaggacct gttcgatctc gcccatgtgg tgatcgcgca
     4801 tcgcccggga tttcggctcc aggaactgca ggccgcgctg cctgcaccgc tgcgaaaaat
     4861 ctacctgcag cgcctggccc gcgtacccgg catgctgcgt gcgaacgccg ggtcaattct
     4921 tacccgcgaa atcactgcgc tcgacatctc ggccactcac atccgcgcca tgctggcgcg
     4981 gggctccagc ccacgctatc tggcccccga taccgttctg gaatacattg accacaacca
     5041 gctttacagg gatcgcgatg cacgttgaag ccatcaagca ggccgtcgtg gaagcgctcg
     5101 aggaaatcaa ggcggccgat atcacggtgc tggacgtaag gcagatgacc tcgctgttcg
     5161 actacatgat catcgccagc gccggatcaa cccggcagac caaggctctg gcgaacaacg
     5221 tacaggacaa gctcaggcag atcggcgcgc gcgtgctcgg tgtcgaaggc gagcagacgg
     5281 gcgagtgggt gctggtggac ctcggcgaaa tcatcgtcca cgtgatgcag ccggcggtgc
     5341 gcgcctacta caacctcgag cagttgtggg gcgggacgcc accctcgccg cacccgacca
     5401 attggcgaaa cgcgcctgga gccgagcctt cacgcgctct gtaaccatcg gcacgctgcc
     5461 cgccttgtga agctgcacgt cgtcgcggta gggcgcaagc cgcccgactg gcttgcggcg
     5521 gggttcgagg agtatgcccg ccgcatgccg cgatccgcac gcatcgatct catcgaggtc
     5581 aagcccgccc cccgcggccg tgagaccccc tccgaagccc tggtctcccg ggtccttgcc
     5641 gaagagaaat cccgcatttt cgcggccatt ccctccggct gtgtaaaaat agcgctcgac
     5701 gaacgcggga aacagctctc caccgcggag ttggcccgca ggctggacgg ctggatgcag
     5761 ggggggcgca atgtcgcatt cgtgatcggc agcgcggacg ggctggatcg ggacctgaaa
     5821 gcgggcgccg atctcttgct gtcgctctcg gcaatgacgc tcccgcacgc gctcgtacgc
     5881 gtgctgcttg ccgagcagct ctaccgtgcg gtttcgctga tccagaatca tccgtaccac
     5941 cgcgattgac ggcgccgcat ctgggcgcta accgataacg agatctcgaa aacaacaacc
     6001 gtgccggcgc tggaaaaaca catctacctt gcctcgcgca gcgcgaggcg ccgcgatttg
     6061 ctgaagcaga tgggcgtgaa cttcgaaatc ctgcttctgc gcgaaggtgc tggacggaac
     6121 gcggatttca acgagtccga gcttccgggg gaaacaccgc gcgagtacgt ggtgcgggtg
     6181 gcgcgtctga aggccgaggc gggctggctg cgcctggagc agcgccgtct gatgcgccat
     6241 ccggtgcttg ccgccgacac cacggtcgcc ctgggcgaca ccatcctcgc caaacccaag
     6301 gaccgcgagg atgccgtggc catgctgaag cagctttccg gaaccgttca tcacgtctat
     6361 acggcgatgg cgatcaagtt ctacgactcg cttcaggaag cgctgtcggt gaccgaggtg
     6421 cgattccgcg aactctccga cgacgaaatc cggcgttacg tcgccaccgg ggagccgctg
     6481 gacaaggccg gcgcttacgg catccagggc aaggcatcga tgttcatcga ggccatcaac
     6541 ggcagctaca ccggggtggt cggcctgccg gtattcgaga ccgcgcggct gctgggccac
     6601 ttcggcttcc agctcctgtg aacgaagaaa tcctgatcaa cgtcacgccg caggagacgc
     6661 gggttgcgat gatgcagcag ggcgtggccc aggaactgca catcgagcgc agcggcagcc
     6721 gcggtctggt cggcaacatc taccttggcg aagtgagccg ggtgctgccg ggaatgcagt
     6781 cggctttcat ccagatcggg ctggaccgcg cggcattcct gcacgtggcc gacatctggg
     6841 gccaccgcca gaaccacgac gagtcgaagc cgatcgaacg catcctgcac gacggccaga
     6901 agctgctggt acaggtcatc aaggatccga tcggcaccaa gggcgcgagg ctttccaccc
     6961 aggtgagcat cgccggacgg ctgctggtct acctgccgca ggaatcccac atcggcatat
     7021 cgcagcgcat cgaagacgag gccgagcgcg cgctgctcag ggaaaagctg caacatctca
     7081 tcctggcgga cgaaccgggc ggcttcatcg tccgcaccat ggctgaaacg gcgagcgaga
     7141 aggaactcgg caccgacatc gagtacctgc gcaaactctg gtccgacatc aaggagaagg
     7201 ccgccatcgc caccgcgccg gcgctgcttt accaggacct gacgctgagc ctcagggtgc
     7261 tccgcgactt cgtcaacgac cgttgcgatc gcgtcctggt ggattcgcgc gaaaccttcc
     7321 agcgcatgca ggcgttcgcc aacgactaca cgctgtcctt cgccgaccgc atccagcatt
     7381 actcgggcga ccggccgctg ttcgatctgt acggcgtcga agacgagatc gagcgggcgc
     7441 tggcccgtcg cgtgccgctc aagtccggcg gttatctcat catcgaccag accgaggcgc
     7501 tgactaccat cgacgtcaat accggcggtt tcgtcggtgg ccgcaccttc gacgacacca
     7561 tattcaagac caacctggaa gctgcgcagg tgatcgcgcg ccagctcagg ctgcgcaacc
     7621 tgggcgggat catcatcctc gacttcatcg acatggacaa cgaagtgcac cgcgatgcgg
     7681 tgctctccga gttcaggaag gcgctcgcgc tcgaccgcac ccgcatgacc gtgaacggct
     7741 tcacccagct cggcctggtg gagatgaccc gcaagcgtac ccgcgagagc ctcgcccaca
     7801 tcctgtgcga gccctgcccg acctgccagg gacgcggcga ggtgaaaacc gcgcagacga
     7861 tctgttacga aatcctgcgg gagctgttgc gcgaggcacg ccagttcgac gctcgcgagt
     7921 ttcgcatcct cgcctcgcag atggtaatcg acatgtttct cgacgaggaa tcgcagagct
     7981 tggcgatgct cgaagaattc atcggcaaac ccatttcact gcaggtcgaa acgatctaca
     8041 cccaggagca gtacgacatc gtgttgatgt agcgccagga tccggcgcgg ttcagggaaa
     8101 ggaaaacttg ggatttgtgc aggccatcaa tccgccgcca gcccggcggc cttgagccgt
     8161 cccctgagac gccccacttc ctcgatcaaa tccactacga gtgcggcgag ttcctggttg
     8221 gcgtcgaaat cgcgttccaa cgccgcaagc cggcgcgcgc gcgccaggtc ggcactgacg
     8281 aagcgccatt ccacagtttt caccgaagca ctgcccagga ggccggcctc tacccgctcc
     8341 acgacccaac gggactcgac cgcgcaggcc cgggccaggt cttccgcgtt cagcgcgact
     8401 tcgtccagta aaacaccggt caggatgtca tcttgtgcca tggctcacac ccccaatttc
     8461 tgacgcggat tgaacgcaag ctcccgcgcc atggtctggt aaatctgtcg cgccttgtcc
     8521 gtgtcggcgg gcggcagcac cacttccagc accagataca gatctcccgg gggattcccg
     8581 ggaatgccgc gccctttcag gcgcagcttg cggcctgcct gggagccggc gggaactttt
     8641 acctgcacat tgcccgaggg cgtgggtact tcgatggttg cgccaagagt ggcttcccac
     8701 ggggccaccg ggactgactc gtacacatcc cgcccttcga cgcgaaagcg cggatcggga
     8761 ctgaattgca cttccagata caaatcgcct gccggacccc cgcccatgcc ggggctgcct
     8821 tgtcccgcga ggcggatgtg ctggccctct ttcacaccct tggggatctg cacattgagg
     8881 gcgcgctcct cggtgatcac atggcccgag gcgtcctgct tcgccgcacg cagggtgatc
     8941 gtccgcgtcg ccccgtgata cgcgtccgcg agaccgatca ggatcctggc gtggtgatcc
     9001 ccgccacgca tctgaaagtc tccggcgccc cggtcgcggc cggcgcggcc gaagaggctg
     9061 gcaaagaagt cgctgaagtc cgccgccccc ccgctgtgag cgccgtcgcc ggaaaactcg
     9121 aatcccgcgt cccagccggg cggagcctga aaatcctgtc ccggctgcca gcggctgccc
     9181 agttggtcgt aggccgctcg cttttcagga tcggaaagca ctgcgtaagc ctcgttgacc
     9241 tccttcatgc gcttttccgc atccggctcc ttgcttacat ccggatggta cttgcgggcc
     9301 agcttgcggt aggccttcct gatttcatcc gccggcgcgg tcttctcgac accgaggatc
     9361 tggtagtaat ccttgaattc caccggtcac cgccgtcagg ccaccttcgc caccttcgcc
     9421 gtcgccggct cgcgcgccag cacgtcgcgg atccgggtga gctcctcgac ctgtttgccg
     9481 agttgcaatt gctgctgctc cagctggcgc acgcgctcct gcagcgtgtt gatgtccccg
     9541 gcgatcttgc gcaggttctc cactcgccgc tccagcagct tctgatgttc cttgagctgc
     9601 atcgacagcg gcaacagcgc cgacctgatc cacgagtcca gctccactcg cagcgcgcgg
     9661 aacaggtcgc gcgcatgcgc aaccagtccg ttgtaaaaac gccgcaccag gaagtgcttc
     9721 tcggtcatca cgttgaccgg atccttgcag aagcgttcgg tgctctgttg cagcgagtgc
     9781 atgcgcagga tgtggcgttc gagattgagc gccggcgggg tcatcttgcg gaagccgtac
     9841 attttgtgga actcgttgta caccccgtcg acgtagttgc gggtttcgcc ggcgaagttg
     9901 aggattttct cggcctggcg cgaaaaggtg tcgaacagcg cctgcatgct gcgcatcagc
     9961 ccggcggtgg tccagctgcc ctcgatcgtc tgctggctct tgcgcatgat gtcgtcgagg
    10021 cgttcgtcgg acagcgtgtc gagaagtacc tgcccctgct tgagcactgt gccgtaggag
    10081 tgcttgaagc tctccatgtg gcgcaggtaa ttggccctgt cggcctccag cttggcgagc
    10141 agcaatttcg caaggtcgcg attcttgccg gagagcttgg acatttcctg cagttcgctc
    10201 ttggcgctgg ccagttgcgc cgtcaccgac tgcagcgacg tttccaccat gccgccgatc
    10261 tcccttgaaa ccgcggcgcg caggatctgc tgtttcgacg ggatgatttc ttcggccagc
    10321 aggcgttcga gatcgccgat acggcttctc ctcagcagct cgtcgtcgtc gcggatgcgc
    10381 gcaatcaggc ccttctgtgc tgacagcgcg atgacatggt cacgcgggag ttcgagtatc
    10441 ttcgcggtct cgtcgacctg cctcccgatt cccacctcga tctgatcctc gctcttgagg
    10501 tcgtcccaca tcaggtcgat cttgttgaga acggcgatgc ggcgggtgac atagctctgc
    10561 acgtacttct gccagacgtc cagatcggac ttggtcacac ccgtgtccat cgccagcagg
    10621 aacagcaccg cgtgcgcgtt ggggatcatg gaaagcgtca gctccggctc ggtgccgagg
    10681 gcgttcaggc caggcgtatc gaggatcacc agtcccttct tgagcagcgg gtgcggatag
    10741 ttgacgagcg catggcgcca acacggaatt tccaccctgc cgccgtgctt gaccaccacc
    10801 gtggtggtca cgaagtccga ttcgtcgagc aggcccagcg ccccggcttc ctcgaccgtt
    10861 accgacttga cctgggcgag actggacatc gccttggtca tttcctcgtg ggaactcagg
    10921 tcgagccgga tcttcaccca ctcgaccgtc tggtgcttca gcgcggcgat gctttcgtcg
    10981 ctcttgcgcg attcgatcgg caacagcttg atgtacggtt cctcggtggc gtcgtagtag
    11041 atttcggtgg ggcacatggt ggtgcgcccg atatctgaag gcagcagacg ctgcttgaaa
    11101 tcggaaaaga acatggcatt gatcagctcg gtcttgccgc gcgaaaactc ggccagaaaa
    11161 gcgaggatca tgcggtcgtt gcgcaggctc tcggccagat cgtagatgcg caggctgcgc
    11221 tgaatatcgg cgtggccgtg gttctcaagc caggattggt acgcctctat gcaggcacgc
    11281 agttcgtcgc gccactgctg gtactgcgtt acctggttct ctaggtcgct gatcttcatt
    11341 gcaggagctc tttcaatcga accagcaggg tctgccccgg gttcgagatc tcgatggtct
    11401 tggtacgccc gcggctgcca cgcgtaatga taacccgccc gcccggcaga tcaaattttt
    11461 tcttaagaaa atcaaggagc agcgcgttgg ctcggtcctc gattgcgggg cccgcaacgc
    11521 gtattttcgg gtacccgcca tggaacccgg cgaactcggt gcggctggcg ttaggctgga
    11581 cccgaacaat gaatctaagc aagtcccgat ccgggtcgta gctgtaccag gattcggcca
    11641 agcgtcacag catccggctg aaggtgatta tcagcggcgc aaccggccac atcagcaaca
    11701 actggcacaa aaccagtacg aacagcggcg atagatccac cccgccgaca ggagggatgc
    11761 ggcgctggaa cggccgcagg aaagggcgcg tcaggcttac cagcatactc attgccggac
    11821 tgtgcggatt cacccatgac aggatggcct gcgcgataac cgccaccatg agaatgtaca
    11881 gaaagcgctt caacaggttg accacggcca acaaaatcaa tcccacgatt gccgcgccca
    11941 ctgccggcgc catttcgaag ccgctcaagg ccctgatcga cacgatcaga agtacttcga
    12001 ccgcccacgc cagcagcagc gtggacatgt cgtaacccca cagaccgggt atgacgcggc
    12061 gtgccggccg gaccatgaaa tcggtcagtg cagcgaggaa ctgagacaac gggttgcgcg
    12121 ccggcgcgcg cactgcctgc agataaaagc gcagcaaaag cgcgagcgcg aacaaaccca
    12181 gaaaagtctc aagcaggaat accaatgcct ggttcatctt tgcgaagtcg atcttctaga
    12241 ttttgccgta ttcgtcgccc agctcgcccg aacgccgggc tgcggcgcgc accgcgtcca
    12301 ctacgcggcc cttcaattcg caggactcca tgacctcgag tgcacgttcg gttgtaccgc
    12361 ccttcgaagt aacgtgcgca cgcagtactg caggggggtc ggtgctgccg gccgcaagtt
    12421 tcgccgcgcc cacaaacgtc tccacggcaa ggctgcgcgc ctgctcctca ctgaacccga
    12481 gttcctgcgc cgcctgctgc aacgcctcga taaagtagaa cacgtaggct ggaccgctac
    12541 ccgacaccgc agtcacgccg tccatgcggc cctcgtcatc cagccagatg accgtgccga
    12601 ccgcacccaa tacacgttgt gcggttttcc tgtcggcctc agtggccttc ggtgccgcat
    12661 acaacccggt gatgccggca cgcatgagtg ccggggtgtt aggcatcgcc ctcacgatcc
    12721 gaccatgacc gccaagccag cgcgacaggt ccgcggtacg aatgccggct gcgatgctca
    12781 ccaccaactg tccgtgcaga tgtggacgca accattgcgc cacgtcgcgc aaatgctgcg
    12841 gcttgaccgc aagcagcacg acgtcctcca cgacgccggc ttcatcgagc cctgcctgcg
    12901 tttccacgcc aagcgcctgg cgcagacgat ggcgcgcatc ctcggacacc tcgacgacgc
    12961 gaatgtcgct ggccgagagc ccctgcttga gcaggccgcc gatcagtgcg gaggccatgt
    13021 tgccgccacc aatgaaaaga atgcgcatgt tgagaagctg gaatccgttt cgatagtggt
    13081 tacgttggcg ccggcactac attgccatcg gcacaccgcc gcggttgggc gacttcgtcc
    13141 gccaaccagt atataggcgg ccgctatccg gcgccgcgtt cgccgaagat ggcgcgaccg
    13201 acacggacca cggtcgcgcc ctcggcaatt gccgcttcga ggtcgtcgga catgcccatg
    13261 gacagcgtat cgagcgcgac tccccggcgc agaaagtcgt cccggatttc gcgcaacatg
    13321 cggaagcggc tgcgcagcag cgccgtgtcg gcggtcggtt cggggatcgc catcagaccg
    13381 cgcaggcgca ggccgggcag ccgcatgatc gcctgcgcga ggccgaaaac cgcatccggc
    13441 accacgccgc ttttgctggc ttcaccactg acgttcacct ggatgcagac ctgcagcggc
    13501 ggcctgtcat cggggcgcga caccgacagg cgctcggcta tcttcgcgcg gtcaatgccg
    13561 tggacccaat cgaaattctc cgcaatcgcc ctggtcttgt tgctctggat cgggcctatg
    13621 tggtgccaca ccatagggcg gagcaggggc ccctctgggg atgtgacccc ggcggagggg
    13681 cgcacgtcgc ccggagttct ttcgtcatct tgattcggtg atgcgacgtg ccagagcagg
    13741 ggccgttccg gggatgcgtt gcgctgccgg aggaggtcga aaagcgccgc aattttctcg
    13801 atcccttcct gcgcatagtt ctcgccaaat gcctgctggc cagcggcgta ggcttcggcg
    13861 attgccttgg gcgggaaggt cttgctgacc gcaaccaagg tgatctcccc gggcgcgcgc
    13921 ccggcgaccc tggctgcctc ggaaatacgt gccttaacag cttgcaagcg ggatgctatt
    13981 gttgtcataa tcgtcttgca gatttcaatg taagcatcga atgcagcaag cctttttgat
    14041 ccgcagcgtg gccaccggca cgctcttcgc acccccatct tgcctttgtc ggggaattat
    14101 aaatggacat ttctgaactc ctcgctttcg tcgtcaagaa caaggcttcc gacctgcatc
    14161 tgtcggccgg gcttccgccc atgatccgcg tccacggcga cgtgcggcgc atcaacctgc
    14221 cgccgatgga gcacaaggac gtgcacgcga tgatctacga catcatgaac gacggtcagc
    14281 gcaagtttta cgaagagaac ctggagtgcg acttctcgtt cgccatcccc aatctggcac
    14341 gattccgggt caacgctttc gtgcagaacc gcggcgccgg cgcggtgatg cggaccattc
    14401 cgtcgaaggt gctgacgctg gaggaactga aggcgccgaa cattttcaag gagattgcgc
    14461 agtatccgcg cggcgtggtg ctggtgaccg gccccaccgg atcgggcaaa tccaccacgc
    14521 tcgcggcgat ggtcaacgac atcaacgaga acgagttcgg ccacatcctt acggtcgagg
    14581 acccgatcga gttcgtgcac gagagcaaga aatgcctgat caaccagcgc gaagtcggac
    14641 cgcatacgct gtcgttcaac aatgccctgc gaagcgcgct gcgcgaagac ccggacatca
    14701 tcctggtggg cgaattgcgc gacctggaaa cgatccggct cgcgctgacc gcggccgaaa
    14761 ccggtcacct ggtgttcggc acgctacata ccagctcggc ggcaaagacc atcgaccgga
    14821 tcatcgacgt gttcccggcg gcggaaaagg aaatggtgcg ggcgatgctg tcggaatcgc
    14881 tgcgcgccgt gatctcgcaa acgctgtgca agaccaagga cgggggcggc cgcgttgcgg
    14941 cgcacgaaat catgatcggc accccggcaa tccgcaacct gatccgcgag gccaaggtgg
    15001 cacagatgta ttcggccatt cagaccggcc agaatctggg catgcagacg ctcgaccaaa
    15061 atctgtcgga catggtgaag aaaaacctca tcacgaaaga ggaagcccgc ggcaaagcgg
    15121 ccagcaagga cgctttcctc tgagcgcacc gtgcggcgac tctgaaaacc ctgccaggag
    15181 cattagttag atggaaaaag accaggccgt cagattcata cacgaactgc tgagggggat
    15241 ggtggcgaga aaggcatcgg accttttcat caccgcgggt ttcccgccgg cgttcaaagt
    15301 cgacggcaag atgacgccag tgtcgaacca gagcctgacc ccgcagcaca cccaggaact
    15361 ggcccgctcg ctgatgaacg acaaacaggc acaggagttc gaacgcacca aggagtgtaa
    15421 tttcgcgatc aacccacccg ggatcgggcg ctttcgcgtg aacgccttcg tgcagcagca
    15481 acgcgtgggc atcgtgctgc ggaccatcac cactaccatc cctaaattcg aggacctcgg
    15541 cctgcccccg gttctgaagg acgtggcgat gaccaagcgc ggactggtga ttttcgtcgg
    15601 cggtaccggc tccggcaaat ccacgtcgct ggcggcgatg atcggctacc gcaacgagaa
    15661 cagctacggc catatcatca cgatcgaaga cccgatcgag tacgtgcatg accacaagaa
    15721 ctgcctgatc acccaacggg aagtcggggt cgataccgac tcctgggaag cggcgctgaa
    15781 gaacacgctg cggcaggcgc ctgacgtgat cctgatcggc gaaatccgcg agcgcgagac
    15841 catggagcac gccatcgcct tctccgagac cggacacctg tgcctgggca ccatgcacgc
    15901 caacagcgcc aaccaggcgc tcgaccgcat catcaacttc ttcccggagg aacgccgcgc
    15961 ccagttgttg atggacctgt cgctgaacct caagggcctg atctcgcagc ggctgattcc
    16021 gctcaaggac accaagggcc gctgcgcggc gatcgagatc ctgctcaact cgccgctgat
    16081 ttccgacctg atattcaagg gcgaggtgca cgagatcaag gagatcatga agaagtcgag
    16141 ggaacttggc atgcagacct tcgaccaggc gctgttcgag ctctatgaga tcggaaaaat
    16201 cagttacgag gacgctatgc gaaacgccga ctcgatgaac gaactgcgac tgcagatcaa
    16261 gctgcacggc aaggaagcga aggaccgcga cctcaccacc ggggtggatc acctgaatat
    16321 tgtttaggga tgagggacga ggggcggggg gcgagggaag atctgcaaag caaagtttct
    16381 ggtccttcat tttccctatt ccttgatccc tgcttttcct tagtccctgc tttcccctca
    16441 cccctcgtcc ctcgcccctc gtcgctgttt ctcccttcgc atcgcgccgg cgacgatctt
    16501 gaactcgtac agccggcact ccagcggacc gttgtaaagc ggcgtgcgct tcgacggcga
    16561 cagcccgatc agcttgggta ggcgcaagtc ggcagtaaac acacaggcgc gccagccggc
    16621 aaacttcttt ttcaaaacgt tccccagctg cggatagaac gcggccagtt tctcctgctc
    16681 gcccaggcgc accccgtaag gcggattggt caccaacacg cctccggccg ccggcgccga
    16741 gatttccagc acgttggcct gtttgaggtt gaccgcatcg tctagtcctg ccgcagccaa
    16801 gttctcgcgc gccagcttga gcgcgtctcc gaacagatcg ctgccgaaga tcggcgtccc
    16861 tgcgtcattc ctgatacacg cttcagcttc gtccctgatg ctgacccact tcgcagcatc
    16921 gaagctgcgc agtttttcga acgcaaacct gcgattgcgt ccggccggaa tgccacgcgc
    16981 gatctgcgcc gcttcgatca gcagcgtgcc actgccgcac ataggatcga gcaacggcgt
    17041 tcccggcgtc cacccggcga gcttcaggat tccggcggcg agattctcgc gcagcggagc
    17101 ttcgcccgcc atctgcctcg atccgcgctt gaacagcggc tcgcccgagg tatcgaggta
    17161 aatcgtgaac cgctccacat cgagaaatgc atggatgcgc acgtcgggct gcgcggtgtc
    17221 cacgctggga cggcggccgg cttgcgcccg gaacttgtcg caggccgcgt ccttgatccg
    17281 cagcgtgacg aattccaggc tcttgagcgg gctcgcgacc gccgcgacat tcacccggat
    17341 ggtgagcccg ggatcgaaca ggcgctgcca gtcgagagcg taaacgccga gatagacgtc
    17401 ttccgcgttg cgatagtggc cggagccgat ctcccacagc acccggctgg cgatgcggct
    17461 gtgcaggttg gcgcaatagg cgagcgacag tgttccctta aagcccactc cgccatccac
    17521 cgcgccgatg tccgcggcgc ccagttcgcc gagttccgca gccagcaatg cctcgagccc
    17581 tcggggacag ggggcaaaga atcgttcttg cttcaaaaag aatcacccat tcaagacttc
    17641 accgcaaaga cgcaaagaac gcaaagaaaa gcagaaaaat agatggggaa tgtgcagaac
    17701 ccgctttcgt cgcgagtatc ggctaaccgg ttatcgaacg ttatgaaccg ttctttcccg
    17761 ttatccgggt ttcctttgcg ttctttgcgt ctttgctgtg aagatttcga gttttaaaga
    17821 ccgagaaaga tctcgaattc gatgcgtttc cagggatcgc ggtgtttggt caggcgcacg
    17881 atgcgcgctt gcagtgggtt gccgcggtcg agctggagcg ccaccgcttc attctccttg
    17941 cgcggcacgt agccgagctt gtggccgttc cactcgactc tcactgccaa cgggtcatgc
    18001 gggttgtccg gctccctgat caacgcgagc gcgtcgccaa ctttcatctg tccccacagt
    18061 tccttgccct gatagtactg gaagccggca agcggagagt tctgcaccag gatgcgcgcg
    18121 gtcacctgca ccgcctgcag cgggcacgcg caccataagg ccgcgatcag aaggccgcgc
    18181 acaagcgctg tcatgggcct cagaacggct gcaattccac cagcagcacg atggctacca
    18241 gcagcaccac cgggaattca ttgagccagc ggtagaagac gtggccgtgg cggttacggt
    18301 cgcgcttgaa gtcgaacatc aacctgccgc aatacagatg gtaggcgacc agcaccgcga
    18361 ccagcgccag cttggcagcc agccagccac cgtgaaagcc gtatcccagc cacaaccacg
    18421 cgcccgacag caaggtcaat acggcgccag gcgtcatgat gccgaagaac agcttgcgct
    18481 ccatgatctt gaaacgttcg atgctcggcc ggtcctcgct catcgcgtga tagacgaaca
    18541 gccgcggcag gtaaaacagc cccgcgaacc aggtgaccat gaagatgatg tgaaacgact
    18601 tgatccagag catcgcctcg cctcagaaga acaggcgccg ccgcgtcaga accagcgcaa
    18661 catagaacgc gaccgccgcg taacccagca gcaccaggac gtgaaatgcc ggctcggccg
    18721 gcgcgacgcc gacgaccagc ggacgggcca actggacggc atgggtgagc ggcagcaccg
    18781 cggagaccgc ctgtagtgcc ccgggcagct ggtcggtcgg gaagaatacc ccgcagagca
    18841 gcaccatcgg cgtaatcagc agcgtaaagt aataaagaaa gaaatcataa ctcggcgaaa
    18901 ccgcgttcat caccagtccg aggcaggcaa acgtcagccc caccaggaag gcgagcggaa
    18961 cgatccacaa cgtcagcggc gcgtgcgaca gcccgagcgc ccagatgatg atcaggatcg
    19021 cgcagccgga aagaaaactc ttgctcgccg cccacaccgc ttcggcgagt agcacgtcgt
    19081 cgaggttgat cggtgcattc atgatcgcat cccaggtcct ctgcacgtgc atgcgagaaa
    19141 atgcggaata cagcgcctcg aaggtcgcgg tgttcatggt gctgtagcac accgtgccgg
    19201 cggccaggaa agtgatgtac ggcagtccgc cgaccttgcc gaccagcgcg cccaggccat
    19261 agccgagccc gagcatgtac agcatcgggt cggcgagatt gccgagcatc gagggaatcg
    19321 cgagcttgcg ccagaccagg aagttgcggc gccatacgtg tgcgaaacgc aggctcagga
    19381 ccgggggacg gaaatctttc gcgcgcatcg ggtttctagc actcctttct gccgtttttc
    19441 aggttcaccc ttcccccttc acgttttctc tcaaacttag tccctcaggt cgcgtccggt
    19501 cagtttcaga aaaacgtctt ccagatttgc cggccggtgc agatagcgca agccaggacg
    19561 attctgcagc gtggcaatca gcggctcggc gcgggtggta tagcagaaca cggtttcgcc
    19621 ggtatgctcg gcacgcatgg caagtttgtc gccgtgctcg gccgcccatc ccgcaacgtc
    19681 ttccccgtat acctccacca cctccggctc gatgtgacgc gagatcagtt cgcgcgggct
    19741 gccggtggag atgatgcggc cgtggtccat gatcgacaag cgccggcaca ggcgttccgc
    19801 ctcgtccatg taatgcgtgg tcagcaggat ggtcttgccc cgattcagca attgcttgag
    19861 ccgctcccag atgagatggc gggcctgggg gtcgagtccg gtggtcggct catccaggaa
    19921 tatcaggtct gggtcgttga tcagcgcccg cgccagcgac aagcgccgct tcatcccgcc
    19981 ggagagggtc tggatcttcg aattcgcgcg attggtgagc ccggcaaatt ccagaagttc
    20041 ggggatgcgc gcgcgcgtga cggcgtcggc ctggccgaag taacgcccgt acaccagcag
    20101 gttttcgagc accgtgaagt ccgggtcgag gttgtcgagt tgcggcagta cgcccaggcg
    20161 gatacgggct tcgcgcgcgg cttgcggcac agggaggccg agcagcgaga tctcccccgc
    20221 atccggatcg gcaagtccca ggcacaggcg caacgtggtg gtcttgcccg caccgttggg
    20281 cccgagcagt ccgtggcact cgcccgcctc cagcgagagg cttaccccgc gcacgacctc
    20341 gaggttgccg taggacttgc gcaggtcttt tacttcaaga agcggcatct gaaatacaga
    20401 ttaaatcaaa agcgttgacg caaaggtcgc gaagaaaaaa gaaagggcgc tacggggatc
    20461 aggcgcgaca gtgttgctcg acgagtttct gcaaggtcac cagattcccg tggaaaaaat
    20521 gaccggtgcc gggaaacacg acgatcggga gctgttgcgg gcgggcccag tcgagcgcgt
    20581 ccttgagcgg caccacgtcg tcctcctcgc cgtgcaccac cagcgttccg gcgggaaccg
    20641 tgccgaccgg gaagcggccc accgcgggcg ccaccagcac cagatgcttc gcctcgagtc
    20701 ttccggcgac ggaggcggct acgaagccac cgaacgagaa tccagccagc gccagttcac
    20761 cctcgccata agtgcgccgc gcatgctccg cgagcaccag gaaatcctcg atttcgccgc
    20821 ggccctcgtc gaacacgccc tcgctggctc cgacgccgcg gaaattcata cgcagcgcgc
    20881 agtaccctga cgcgaaaaac gctttcgcca gtgtctgcac gaccttgtta tcgagcgtgc
    20941 cgccgtacag cggatgagga tgcgcaacca gcgccacgcc gcggcgcgcc tccccgggat
    21001 cgttggcgac gatctcgagc ctgcccgccg gtccgtcaat cgagagtcgg ctctgtgtta
    21061 cagatttcaa atgcgcaggc gctcgacgat gcggccgtgg accagatgct cgtcgatgat
    21121 ctcatcgatg tcgctcttgt ccacgtaggt gtaccagatc tcctgaggat aaatcgcgat
    21181 caccgggccc tcgtcgcagc ggtccatgca gcccgcctgg ttcacccgca ccttgcccgg
    21241 gcctgcgagc tgcagcgact tgacgcgggc cttggcgtaa tcgcgcattt cctgcgcgtg
    21301 atggtt
//
LOCUS       DASQOP010000233         6233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1317702, whole genome shotgun sequence.
ACCESSION   DASQOP010000233 DASQOP010000000
VERSION     DASQOP010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6233
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317702"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1309
                     /locus_tag="VE030_09485"
     CDS             <1..1309
                     /locus_tag="VE030_09485"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZF23684.1"
                     /translation="QTDETDLYAAMDWLLERQEFIEKKLAARHLSEGALALYDLSSSY
                     FEGNSCPLAKIGYSRDGKRNTPQVNYGLLTTRAGCPVAISVYEGNTADVSTLMPQVTQ
                     LREQFGLERLVLVGDRGMISHKAINELRALDGLAWITALKSAQIRALVQGGELQLGLF
                     DERNLFEFSHPDFPNERLMACRNVDLGKLRSHKRQALLEATEKELQKVRARIENGSLA
                     GRDKIGVRVGKVLNKYKVGKHFALTIEETGFEFHRLEQQIAAEAALDGLYVIRTSVPK
                     KQMTSAEAVRSYKALAEVERAFRSMKTIDLHIRPIHHRLESRVRAHIFLCMLAYYVEW
                     HMREAWRELMFADEDLERKKHRDPVAAAERSEAALDKVATRKLKNGSPVHSFRTLLEE
                     LSTIVRNTCEARVGQRSGSSFQMTTMPNPAQQRAMQLLQSITV"
     gene            complement(1435..1510)
                     /locus_tag="VE030_09490"
     tRNA            complement(1435..1510)
                     /locus_tag="VE030_09490"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1475..1477),aa:Thr,seq:cgt)
     gene            complement(1534..2514)
                     /gene="ispH"
                     /locus_tag="VE030_09495"
                     /gene_synonym="lytB"
     CDS             complement(1534..2514)
                     /gene="ispH"
                     /locus_tag="VE030_09495"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018026382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HZF23685.1"
                     /translation="MQIYLANPRGFCAGVDRAIDIVERAVVLFGAPIYVRHEVVHNKY
                     VVDRLRTIGAVFVDELNEVPDGATVIFSAHGVPRVVQQEAKRRLLNVFDATCPLVTKV
                     HMEITRYAREAREVFLIGHAGHPEVEGTIGQFDATLGGAIYLIQTVEDVERIQVRNAD
                     HLAFVTQTTLSVDDTACIVAALRARFPGLKAPVKEDICYATQNRQDAVKDLIHRCEVL
                     VVVGSKSSSNSNRLREMAGKNDKRGYLVDGPEDLHREWFDGVKTVGITAGASAPEILV
                     QGVIAQLRQWGGEVAEELQGRKEHVIFALPKALREVAKDRTSLSSVGPTG"
     gene            complement(2514..3011)
                     /gene="lspA"
                     /locus_tag="VE030_09500"
     CDS             complement(2514..3011)
                     /gene="lspA"
                     /locus_tag="VE030_09500"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019486629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HZF23686.1"
                     /translation="MTIGENRGRAEAWLLLSALVVLADQVSKAYIAGHFLEFEFVRIL
                     PVLDITCMHNVGAAFSFLASASGWQRWVFIGLAVVVSIGITVWLFRLPRGAHPLLAAG
                     LALVLGGAIGNVIDRIRLGYVIDFIHFHWERAYFPAFNVADSAITVGAACLLLDAFFE
                     ARRKN"
     gene            complement(3008..5794)
                     /gene="ileS"
                     /locus_tag="VE030_09505"
     CDS             complement(3008..5794)
                     /gene="ileS"
                     /locus_tag="VE030_09505"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HZF23687.1"
                     /translation="MKADLANREPGMLAAWERSGLYEKIRGVAKGRPSFVLTDGPPYA
                     NGAIHLGHAVNKILKDIIVKSRTLDGYDAPYVPGWDCHGLPIEMQVEKTHGRVGQKID
                     AKAFRAACREYAHKQVDAQRRDFKRLGVLGDWENPYLTMMPRFEAEQLRAFALIIKNG
                     HVYKGFKPVHWCLNCRSALAEAEVEYEDKTSPSIYVRFPVVDSADLAQRFGIEEARKA
                     AGERREVPTSVVIWTTTPWTLPANRAVAVHPQFDYALIEFDLGRGAERLVLAVELIDA
                     VMNALGFTEWSLIATVKGAALEHMLLRHPLYDRQVPVILGEHVTLDAGTGAVHTAPGH
                     GLDDYIVGRRYGLEIDNPVGGDGRFLPSTPLFAGEQVFDANAHVIKVLHDHGRLLKDE
                     PYHHSYPHCWRHKTPVIFRATPQWFIGMEQAGLRKGALEAISHVDWMPTWGEQRISGM
                     IAGRPDWCVSRQRTWGVPLPLFVDKESGELHPRTGELIEEVAKLVEHAGIDAWFDLDA
                     AALLGPDSSRYDKATDVMDVWFDSGVVHHCVSQIRPEIVAPSDLYLEGSDQHRGWFHS
                     SLLTSVAMHGRAPYRGVLTHGFTVDEKGRKMSKSIGNTLVPQKLTSTLGADVVRLWVA
                     ATDYANEMSVSDEILKRMADSYRRMRNTLRFLLGNLHGFDPARHAIAFDDLVAIDQWA
                     IAKAFALQNDVVTAYRNYEFHDIYQKVHNFCVVELGGFYLDIIKDRLYTTGAGSAPRR
                     SAQTAMYHVAQAMVRWLAPILSFTAEEVWGFLPGVGNESVFLNTWHQFPLGAERVPAI
                     DWPALITLKADVARDLERLRAAGAIGAPLEAEVTIYAPAAQAGRFKALRDELRFLLIT
                     SQAHVVESDTPSASTVPTSEPGVWIEVKPSTQPKCVRCWHLRSDVGSDPRHPELCARC
                     VINVEGPGEERQFA"
     gene            complement(5835..>6233)
                     /locus_tag="VE030_09510"
     CDS             complement(5835..>6233)
                     /locus_tag="VE030_09510"
                     /inference="COORDINATES: protein motif:HMM:NF013821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HZF23688.1"
                     /translation="ARLLGRPYRMIGRVVRGRRLGRALGFPTANLRLMRRKPPVWGIL
                     AVWVHGIDSRPLPGVASLGTRPTVNGVEPLLEAHVFDFSGDLYGRAIEVEFVAKLRDE
                     VKFESLDAMMVQMKIDGARARDLLSKVDSG"
BASE COUNT         1276 a         2011 c         1829 g         1117 t
ORIGIN      
        1 gcagacggat gagacggacc tgtacgcagc gatggactgg ttgctcgagc gccaggaatt
       61 tatcgagaaa aagctcgctg ctcgacacct gtccgagggt gcgctcgcgc tgtacgactt
      121 gagttcgagt tacttcgaag gcaatagctg tccgctggcg aagatcggct acagccgcga
      181 tggcaagcgc aatacgccgc aggtgaacta cggcctgttg accacgcgtg ccggctgccc
      241 ggtggccatc tcggtgtacg agggcaatac ggcggacgtg agcacgctga tgccgcaggt
      301 tacgcaactg cgcgaacagt ttggcctcga gcgcctggtg ctggtcgggg atcgcggcat
      361 gatctcgcac aaagcgatca atgagctgcg tgcgctcgac gggctggcgt ggatcacggc
      421 gctgaagagt gcgcagatcc gcgccctggt gcagggcggt gagctgcaac ttgggctttt
      481 cgatgagcgg aatctgtttg agttctcgca tccggacttt cccaacgaac ggttgatggc
      541 ctgtcgcaac gtcgatctcg gtaaactacg ttcgcacaag cgccaggcgc tgctcgaggc
      601 gacggagaag gaactgcaga aagtccgcgc ccgcatcgag aacggctcgc tcgccggtcg
      661 cgacaagatc ggcgtgcggg tcggcaaagt cctcaacaag tacaaagtcg gcaagcactt
      721 tgcgctcacc atcgaagaga ccggcttcga gttccatcgg cttgagcaac agatcgccgc
      781 cgaagccgcg ctcgatggac tctacgtgat ccgcaccagc gtaccgaaga agcagatgac
      841 atcggccgaa gcggtgcgca gctacaaagc gctggccgaa gtggagcgcg cgttccgctc
      901 gatgaaaacc atcgatctac acatccgtcc catccaccat cgtctggaga gccgcgtgcg
      961 ggcgcacatc ttcctgtgca tgctcgccta ctatgtcgag tggcacatgc gcgaggcgtg
     1021 gcgcgagttg atgtttgccg acgaggacct ggagcgcaaa aaacatcgcg atccggtcgc
     1081 cgccgccgag cgatccgagg ccgcgctcga caaagtcgcc actcgtaaac tcaaaaacgg
     1141 ttcacccgta cacagctttc gcacgctgct tgaggaactc tccaccatcg tgcgcaatac
     1201 ctgcgaggca cgagtcggtc agcgcagcgg ttcctcgttc caaatgacca cgatgccgaa
     1261 tcccgcccaa cagcgagcaa tgcagttgct gcagtcgatc actgtgtagc cacacgctcg
     1321 acaccgagaa atggctcaac tccctgatct accgagaaat ccccttcacc ccgaccaaga
     1381 actcccgact agaacactag cggcacgtat atgtctgaaa ttacaggtaa aaactggtgc
     1441 cgcaaagagg attcgaaccc ctgacccacg cattacgaat gcgttgctct accaactgag
     1501 ctattgcggc gtcgacccga aaggggccgc attctacccc gtgggaccca ccgacgaaag
     1561 actggtgcgg tcttttgcga cttcgcgcag agctttgggc agggcgaaaa tcacgtgttc
     1621 cttccgaccc tgaagctcct ccgccacttc accgccccat tggcgtagtt gcgcgatgac
     1681 cccctgtacc agaatttcgg gcgccgaggc gccggccgtt attccgacgg tcttgacgcc
     1741 gtcgaaccac tcgcgatgca gatcctccgg cccgtccacc aaataacccc gcttgtcgtt
     1801 tttgccggcc atttcacgca gccgattgga attggaactg ctcttcgagc cgaccacgac
     1861 cagcacttcg cagcggtgaa tcagatcttt gacggcatcc tggcggtttt gcgtggcgta
     1921 gcaaatgtcc tctttcaccg gcgccttgag acccggaaaa cgggcccgca atgcggcaac
     1981 gatgcaggcg gtatcgtcga ccgaaagggt ggtctgagtg acgaaggcca ggtgatcggc
     2041 gttgcgaacc tggatgcgct cgacatcctc gaccgtctga atcaaataga tcgcgccgcc
     2101 cagcgtcgcg tcgaactgcc ctatggtgcc ctccacttcc ggatggccgg catggccgat
     2161 caagaacacc tcgcgcgcct cccgggcata gcgcgtgatc tccatgtgca ccttggtgac
     2221 caagggacac gtcgcgtcga aaacgttgag caagcggcgc ttggcctcct gctgcaccac
     2281 gcgagggacg ccatgggcgc tgaaaatcac ggtggcgccg tcgggcacct cgttcaattc
     2341 atcgacgaag accgcgccga tggtgcgcag acggtccacc acgtacttgt tgtggaccac
     2401 ttcgtgccgc acatagatgg gtgcgccgaa caacacgacc gcccgctcga cgatgtcgat
     2461 ggcgcggtcc acaccggcgc agaatccgcg cggattggct aagtaaattt gcatcagttt
     2521 ttccgcctcg cctcaaagaa ggcatcgagt aataagcacg cagcgccaac cgtaatcgcc
     2581 gaatccgcga cgttgaacgc tggaaaatac gcccgttccc aatgaaagtg tatgaaatcg
     2641 atcacgtacc ccagtcgaat ccgatcgatg acattgccta tcgcgccgcc gagcaccagg
     2701 gcaaggccgg ccgcgagcaa gggatgcgcg ccgcgcggca gccggaacag ccaaaccgtg
     2761 atgccgatgc tgaccacgac ggcgagtccg atgaataccc agcgctgcca acccgaggcc
     2821 gaagcgagaa agctgaaggc ggcgccgaca ttgtgcatgc aagtgatgtc aaggacaggc
     2881 aggattcgca cgaattcaaa ctccagaaaa tggcccgcga tatacgcctt gctgacttgg
     2941 tccgccagca cgacgagcgc agacaacagc agccaggcct cggctcggcc gcgattctct
     3001 cctatggtca tgcgaattgt cgctcctcac cgggaccctc gacgttgatg acgcagcgcg
     3061 cgcatagttc cggatgccgc ggatcgctgc cgacatcgct gcgcagatgc cagcaccgca
     3121 cgcatttagg ctgcgtactc ggtttgacct cgatccacac gccgggttcg ctggtcggaa
     3181 ccgtggaagc ggaaggcgtg tcggactcga ccacgtgcgc ctggctggta atcaacagaa
     3241 atcgcagttc gtcgcgcagc gccttgaagc gcccggcttg cgccgcaggc gcgtaaatcg
     3301 tcacttccgc ctcgagggga gcgccaatgg cgccggccgc acgcaaccgc tcaagatccc
     3361 gcgcgacatc cgccttgagc gtgatcaagg cgggccaatc gatcgccggc acccgctcgg
     3421 cgcccaaggg aaattgatgc caggtattca ggaacaccga ttcgttcccg acccccggca
     3481 ggaagcccca cacttcttcg gcggtgaaac ttaagatggg tgcgagccag cgcaccatcg
     3541 cctgagccac atgatacatg gccgtttgcg cggagcggcg cggcgcgctg cccgcgcccg
     3601 tcgtgtagag ccgatccttg atgatgtcca gataaaaccc accgagttcg accacgcaga
     3661 agttgtggac cttctggtag atgtcgtgaa attcgtagtt gcggtaggcg gtgaccacgt
     3721 cgttttgcag ggcgaaggcc ttggctatcg cccactgatc gatggctacc agatcatcga
     3781 aggcgatcgc atgccgcgcg ggatcgaagc cgtgcagatt gcccaagaga aatcgcagcg
     3841 tgttgcgcat gcgccgataa gaatccgcca tgcgcttcaa gatctcgtcc gacacgctca
     3901 tctcgttggc gtagtcggtc gcggccaccc acaggcgcac cacgtccgcg cccaaggtgc
     3961 tcgtcaattt ctgcggcacc agcgtgttgc cgatcgattt cgacatcttg cggcccttct
     4021 cgtccacggt gaagccgtgc gtcagcacgc cgcgatacgg agcgcggcca tgcatggcca
     4081 ccgaggtgag cagcgaactg tggaaccaac cgcgatgctg atccgagcct tccagataca
     4141 gatcgctggg cgcgacgatc tccggccgta tttgcgacac gcagtgatgc accacgcccg
     4201 agtcgaacca gacgtccatc acgtcggtgg ccttgtcgta ccgactcgag tcgggtccca
     4261 gcaacgccgc ggcgtccaga tcgaaccaag cgtcgatgcc ggcgtgctcg accagcttgg
     4321 cgacctcctc gatcaactcg ccggttctcg ggtgcagctc gccggattcc ttgtccacga
     4381 acaacgggag gggcacgccc caggtacgtt gtctcgatac gcaccagtcc ggacggccgg
     4441 cgatcatacc gctgatccgc tgctcgcccc acgtgggcat ccaatcgaca tgcgaaatgg
     4501 cttcgagcgc gcccttgcgc aaacccgcct gttccatgcc gatgaaccac tgcggcgtcg
     4561 cacggaaaat caccggcgtc ttgtggcgcc agcagtgcgg ataggaatga tgatacggct
     4621 catctttcag caagcggccg tggtcatgaa gaaccttgat cacatgggcg ttggcgtcga
     4681 atacttgctc gcccgcgaac agcggcgtcg acgggagaaa tcggccgtcg ccgcccaccg
     4741 gattgtcgat ttccaatccg tagcggcgtc ccacgatgta gtcatccaag ccatgtccgg
     4801 gtgcggtgtg cacggcgccg gtaccggcat ccagagtcac atgttctccc aagatcaccg
     4861 gtacttgccg gtcgtacaag gggtggcgca gcagcatgtg ctcgagagcc gcgcctttga
     4921 cggtcgcgat caagctccac tcggtgaacc ctagggcgtt catgacggcg tcgatcaatt
     4981 cgaccgccag caccaggcgt tcggctccgc ggcccaaatc gaattcgatc agcgcgtagt
     5041 cgaattgcgg gtgaaccgcc actgcgcgat tcgccggcaa ggtccaggga gtggtggtcc
     5101 agatgaccac cgacgtcggc acttcccggc gctcgccggc tgccttccgg gcctcctcga
     5161 ttccgaaacg ttgcgccaga tcggcggagt cgaccaccgg gaagcgcacg taaatgctcg
     5221 gcgaggtctt atcctcgtac tcgacctcgg cctccgccag ggccgagcgg cagttcaggc
     5281 accagtgcac gggcttgaac cccttgtaca catggccatt cttgatgatg agggcaaagg
     5341 cacgcagctg ctcggcctca aaacgcggca tcatcgtcaa gtagggattt tcccagtcgc
     5401 cgagtacccc aagtcgcttg aaatcacggc gctgcgcatc gacctgctta tgcgcatatt
     5461 ccctgcacgc cgcacgaaac gccttggcgt cgatcttttg cccgacgcgc ccgtgcgttt
     5521 tctccacctg catctcgatg ggcagcccgt ggcagtccca acccgggaca tagggcgcgt
     5581 catagccgtc caaggtgcgc gacttgacga tgatgtcctt gagaatctta ttgaccgcgt
     5641 gacccaggtg aatcgcgccg ttcgcatagg ggggcccgtc ggtcagcacg aacgagggcc
     5701 ggcccttcgc gacgccgcga attttttcgt acaagccgct gcgttcccac gccgccagca
     5761 ttcccggctc gcggttggca agatcggcct tcatcgggaa acccgtgttc ggcaaattaa
     5821 tcgtatgttt gtaatcagcc actatccacc ttcgacaaga gatcgcgggc gcgggcaccg
     5881 tcgatcttca tttgcaccat catcgcatcc aaggactcga acttgacctc atcacgcagc
     5941 ttggccacga actcgacctc gatcgcgcgg ccgtagaggt cgcccgagaa gtcgaacaca
     6001 tgcgcttcga gcaatggctc gaccccgttg accgtcggcc gcgtgcccag actcgcgaca
     6061 ccgggaagag gacgcgaatc gattccatga acccacaccg ccaagatgcc ccacaccggc
     6121 ggcttgcggc gcatcaatcg caggttcgcg gtcggaaagc cgagcgcgcg gcccagccgc
     6181 cggccacgca ccacgcgccc tatcatccgg tacggccgcc ccaacaggcg cgc
//
LOCUS       DASQOP010000234         2640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_112312, whole genome shotgun sequence.
ACCESSION   DASQOP010000234 DASQOP010000000
VERSION     DASQOP010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112312"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VE030_09515"
     CDS             <1..290
                     /locus_tag="VE030_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZF23689.1"
                     /translation="TFALGSGLAGLGGALGIDVLGLDPTFPVKYMVYFLLVVSVGGAG
                     SIRGTLVAALILGVFDVAGKYYVPEVGAFVIYALMVLLLILFPAGLYGRRA"
     gene            287..1297
                     /locus_tag="VE030_09520"
     CDS             287..1297
                     /locus_tag="VE030_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZF23690.1"
                     /translation="MSVAATVPTPQAASPKPHRLPDGRWHRTEIIFWLLPLAAFFLLS
                     GHLVLGSQILIVALFALSLDLILGYAGIVSLGHAAFFGIGAYTAGLLAVHGWGEALSG
                     LLLAGAMAAVAGYLVSFLVVRGEDLARLMVTLGIGLLLYEAANKAAFITGGVDGLSGV
                     TMWRLLGVFPFDLGGKVAYAYSLAVLFLLFVLMRRLVNSPFGLSLRGLREGGKRMPAI
                     GAPVRRHQVLIFTIAAGVAGIAGGLLAQTTQFVGLEVLGFPRSAELLIMLVLGGAGRL
                     YGALIGAALFMVAQEYLSGLNPVYWQFWIGLLLVVVVLFARGGITGAAEALYTRPRGK
                     GK"
     gene            1294..2046
                     /locus_tag="VE030_09525"
     CDS             1294..2046
                     /locus_tag="VE030_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZF23691.1"
                     /translation="MNSALRTEGLSKQWGSFRANSDLSIALAVGARHALIGPNGAGKT
                     TFINLLTGVFPPTTGKVYLGGENVTYLPQHQRVKRGMTRTFQINTLFPGLTVLESVVL
                     AVCERRGIAGRWMTTVAKQREAVEEAEDLLESLHLLQDADALTRNLPYGKQRLVEIAL
                     ALATRPKILLLDEPAAGIPSAESTELFGVIAGLPADVTILLIEHDMDLVFRFAERITV
                     LVGGQVLTEGTPAEIAADERVKEVYLGEAQHG"
     gene            2039..>2640
                     /locus_tag="VE030_09530"
     CDS             2039..>2640
                     /locus_tag="VE030_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZF23692.1"
                     /translation="MAELLRLDNVSAGYGEAVVLEDISLSVAQGDSLALLGRNGMGKT
                     TLLVTLMGFTRLHRGAIRVLGADVGALPTHRRVHSGLGWVPQERCMFASLTVEEHLTA
                     VARPGYWNLRRVYQVFPGLEERRRNLGNQLSGGEQQMLAIARALMVNPRLLLLDEPME
                     GLAPVIVQDMMAVIRQLLADGGMAVILVEQHAKLALSLTS"
BASE COUNT          396 a          820 c          903 g          521 t
ORIGIN      
        1 tgacgttcgc gctcggttcg ggtcttgccg ggctgggcgg cgctctcggc atcgacgtgc
       61 tcggcctcga tccgactttc ccggtcaagt acatggtgta ttttcttctg gtcgtttccg
      121 tcggcggcgc cggcagcatc aggggcacgc tggtggcggc gctgattctc ggcgtgttcg
      181 acgtggcggg caagtattac gtgcccgagg tcggcgcctt cgtcatctac gcgctgatgg
      241 tgctgctcct gatcctgttc cccgccggtt tgtacggaag gcgggcatga gcgtcgcagc
      301 cactgtgccg acgccgcagg cggcgagtcc caagccgcac cgtcttcccg atggccgctg
      361 gcatcgcacc gaaatcattt tctggctgct gccgctggcc gcgttcttcc tgctgtccgg
      421 tcatctggtg ctcgggagcc agatcctgat cgtcgcgctg tttgcccttt cgctcgatct
      481 cattctcggc tacgccggca tcgtctcgct cgggcacgca gccttcttcg gcatcggcgc
      541 ctatacggcg ggactgctgg ccgtgcacgg ctggggcgaa gcgctgagcg gcctgctgct
      601 ggctggcgcc atggcagcag tcgccggtta tctggtcagc ttcctggtgg tgcgcggcga
      661 agacctcgcc cgcctgatgg tgacgctggg catcggcctg ctgctgtacg aggcggccaa
      721 caaggcggca ttcatcaccg gcggcgtgga cggtctttcc ggagtcacga tgtggagact
      781 gctgggcgta ttcccgttcg atctcggcgg caaggtcgct tacgcgtaca gcctggcggt
      841 gctgttcctg ctgttcgtac tgatgcgccg gctggtgaat tcgccgttcg gcctgagcct
      901 gcgtggtttg cgcgaaggcg gcaagcgcat gcccgccatt ggcgcgccgg tgcgcagaca
      961 ccaggtcctg atcttcacca tcgccgcggg cgtggccggc atcgccggcg gattgctggc
     1021 gcagaccacg cagttcgtcg gcctggaagt gctcggcttc ccgcgctccg cggagctgct
     1081 gatcatgctg gtgctgggcg gtgccgggcg cctgtacggt gcgttgatcg gcgcggcgct
     1141 gttcatggtc gcgcaggaat atctctccgg cctcaatccc gtttactggc agttctggat
     1201 cgggctgctg ctggtggtgg ttgtgctgtt cgcccgcggc ggcattacag gtgcggcgga
     1261 agctttgtac acgcggccgc gaggaaaagg aaaatgaatt ctgcgctgcg caccgaaggc
     1321 ctttcgaagc agtggggcag cttcagagcc aacagcgatc tgagcattgc gctggcggta
     1381 ggggcgcgcc acgcgctgat cggcccgaac ggcgcgggca agaccacgtt catcaatctg
     1441 ctgaccggag tgttcccgcc gaccacggga aaggtgtatc tgggcggcga gaacgtgacg
     1501 tacctccccc agcatcagcg ggtcaagcgc ggcatgaccc gcaccttcca gatcaacacg
     1561 ctgttccccg ggctcaccgt gttggaatcc gtcgtgctcg cggtctgcga acgcaggggt
     1621 atcgccggca gatggatgac caccgtggcg aaacaacgcg aagccgtgga ggaagcggag
     1681 gatctactcg agtcgctgca cctgctgcag gacgccgatg cactcacccg caatctgccc
     1741 tacggcaagc agcgcctggt ggagatcgcg ctggcactgg cgaccaggcc gaaaatcctg
     1801 ctgctcgatg agccggccgc gggcattccg tcggcggaga gcacggagct gtttggcgtc
     1861 attgccggac tgccggccga cgtgaccatc cttctcatcg aacacgacat ggacctggtg
     1921 ttccgtttcg ccgagcgcat caccgtgctg gtgggcgggc aggtactcac cgaaggcacg
     1981 cccgcggaga tcgcggccga cgaacgggtg aaggaagtct atctcgggga ggcgcagcat
     2041 ggctgaactg ctgcgcctcg acaacgtcag tgccggttat ggcgaagccg tggtgctgga
     2101 ggacatatcg ctttccgtcg cgcaaggaga cagcctcgcg ttgctgggcc gtaacggtat
     2161 gggcaagacc acgttgcttg ttacgttgat gggattcacg cggctgcatc gcggcgcgat
     2221 tcgcgtcctc ggtgccgacg tcggcgcgct gcccacgcac cggcgcgtgc attcggggct
     2281 gggctgggtg ccgcaggagc gctgcatgtt cgcctcgcta acagtagaag agcatctcac
     2341 cgcggtggcc aggccgggtt actggaacct ccggcgggtg taccaggtct ttccaggatt
     2401 ggaggaacgc cggcgcaatc tgggcaatca actctcgggc ggcgagcagc agatgctcgc
     2461 tatcgcccgc gcgctcatgg tcaatccgcg cctgttgcta ctcgacgagc cgatggaggg
     2521 cctcgccccg gtcatcgtgc aggatatgat ggcggtcatc cggcaactgt tagcggacgg
     2581 cggcatggcg gtgatactgg tggagcagca cgccaagctc gccttatcgc tcaccagcca
//
LOCUS       DASQOP010000235         2140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_514724, whole genome shotgun sequence.
ACCESSION   DASQOP010000235 DASQOP010000000
VERSION     DASQOP010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514724"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..732)
                     /locus_tag="VE030_09535"
     CDS             complement(67..732)
                     /locus_tag="VE030_09535"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HZF23693.1"
                     /translation="MTRSSAATRFMARIAFAVSCVLACAIAAAGEQKYEPLSASVQAA
                     LHRSVADKASPRLAFASGAEGVTWMADMSKRLEKHVPDATTREDLLTTVHYEATRAGL
                     DPQLVLGVIQVESGFRKYAVSKAGARGYMQVMPFWMTLIGESGHNLFNLRTNLRYGCT
                     ILRHYLDLERGDAFRALGRYNGSLGRSRYPNSVLRAWSGDWEYMAGDRVIATPTVYVG
                     ESR"
     gene            complement(735..>2140)
                     /locus_tag="VE030_09540"
     CDS             complement(735..>2140)
                     /locus_tag="VE030_09540"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HZF23694.1"
                     /translation="RDFCFGPTHEEVITDIARKEIRSYRQLPLNFYQIQTKFRDEIRP
                     RFGVMRAREFVMKDAYSFHAGPQDLAREYRNMYDTYSRIFSRLGLKFRAVAADTGAIG
                     GTGSHEFHVLADSGEDAIAYSPASDYAANVELAEVVAPLTPRGAASQKLQKVPTPGKT
                     TCEEVTAMLGLPLSQSVKALAVMHEERMFMLLVRGDHQLNETKVSKLPGLNPFRFATE
                     KEIEARIGCRPGYIGPVGLPKEITVIADRSVAAMNDFVCGANEAGHHLTGVNFGRDLA
                     EPAQVADIRNAVAGDPSPDGKGRLEICRGIEVGHIFQLGSKYSEAMSATFLDESGKAQ
                     VMEMGCYGIGVSRIVAAAVEQGNDERGIIWPQPMAPFQVAIAPIGYRKNAEVKKAADH
                     MHDELQSGGIEVLLDDRDERPGVMFADLELIGIPHRITVGERGLKDGKVEYQARTDAS
                     ARAIELQSAPNLLKSLL"
BASE COUNT          383 a          657 c          673 g          427 t
ORIGIN      
        1 gtttctctgt gccctctgtg gcaaatgctt tagaaagctg aaagcacgac tagagaagac
       61 gagacgttag cggctttcac ccacgtaaac cgtcggcgtg gcgatcacgc ggtcgccggc
      121 catgtactcc cagtcgcccg accaggcgcg caataccgag ttcggatagc gcgacctgcc
      181 gagcgagccg ttatagcggc cgagcgcacg gaaggcgtca ccgcgctcga ggtcgagata
      241 gtggcggagg atggtgcagc catagcgcag gttggtgcgc aggttgaaga ggttgtgccc
      301 ggattcgccg atcagcgtca tccagaacgg catcacctgc atgtagccgc gcgcgccggc
      361 tttggacacg gcgtacttgc ggaacccgct ttctacctgg atcacgccaa ggaccagttg
      421 cggatcgagc cccgcgcgag tcgcctcgta gtgcaccgtg gtcagaaggt cttcgcgcgt
      481 ggtggcgtcc ggaacgtgct tttccaggcg tttggacata tcggccatcc aggtcacgcc
      541 ttccgctccg gaggcgaacg cgagccgcgg actggcctta tcggcgaccg aacgatgcaa
      601 cgccgcctga acgctcgccg acagcggctc gtacttctgc tcgcctgccg cagcgattgc
      661 acaagcgagt acgcatgaca ccgcgaatgc aatacgcgcc atgaaacgcg tggctgcaga
      721 gcttcgtgtc attctcatag cagggatttt aggaggttcg gcgccgattg caactcaatt
      781 gctctcgccg aagcatcggt tctggcctga tactcgactt tcccatcctt caaaccacgc
      841 tcgccgacgg tgatgcgatg gggaatgccg atcagttcca ggtcggcaaa catcacgccc
      901 gggcgctcgt cgcggtcgtc cagcagcacc tcgattccgc ctgactgcaa ttcgtcgtgc
      961 atgtgatcgg ctgctttttt gacctcggcg ttcttcctgt atccgatcgg cgcaatggct
     1021 acctggaacg gcgccatggg ctgcggccag atgatgccgc gctcgtcgtt tccctgctcg
     1081 actgcggcgg cgacgatgcg cgaaacgccg attccatagc agcccatttc catcacctgt
     1141 gccttgccgg actcgtccag gaaggttgcg ctcatcgcct cggaatactt gctccccaac
     1201 tggaaaatgt gtccgacttc gatgccgcgg cagatttcca ggcggccctt gccgtcaggg
     1261 ctgggatcgc ccgccacggc gttgcggatg tcggcgacct gcgccggttc cgcgaggtcg
     1321 cggccgaagt tcacgccggt caggtgatgg cccgcctcgt tggcgccgca gacaaaatcg
     1381 ttcatcgccg ccaccgagcg atcggcaatg acggtgattt ccttcggcag gcccaccgga
     1441 ccgatgtagc ccgggcggca gccgatgcgt gcttcgatct ctttttcggt ggcgaatcga
     1501 aacggattca ggccaggaag cttcgacacc ttcgtttcat tcagctgatg gtcgccgcgc
     1561 accagcaaca tgaacattct ttcttcgtgc atgaccgcaa gcgccttgac cgactgcgac
     1621 agcggcagtc ccagcatcgc agtgacttcc tcgcaggtgg tcttgccggg cgtgggaact
     1681 ttctgcaatt tctgcgaggc cgcgccgcgc ggcgttagcg gcgcaaccac ttcagcgagt
     1741 tcgacattgg cggcgtaatc cgaagcgggc gagtaggcga tcgcgtcctc gccggagtcg
     1801 gcaagcacgt gaaattcgtg cgagccggtg ccgccgatgg cgccggtgtc ggcggcgacc
     1861 gcgcggaact tcagtcccag acgggagaag atgcgcgaat aagtgtcgta catgttgcgg
     1921 tattcgcgag ccaggtcctg ggggccggcg tgaaaggagt aggcgtcctt catcacgaat
     1981 tcgcgtgcgc gcatcacgcc gaatcgcggc cggatttcgt cgcggaactt ggtctgtatc
     2041 tgatagaaat tgagcggcag ctggcggtag gagcggatct ccttgcgcgc gatgtcggtg
     2101 atgacttcct catgggtcgg gccgaagcag aagtcgcgtt
//
LOCUS       DASQOP010000236         5284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2328425, whole genome shotgun sequence.
ACCESSION   DASQOP010000236 DASQOP010000000
VERSION     DASQOP010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5284
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2328425"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..1124
                     /gene="pdxA"
                     /locus_tag="VE030_09545"
     CDS             144..1124
                     /gene="pdxA"
                     /locus_tag="VE030_09545"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HZF23695.1"
                     /translation="MSSRIGITMGDPSGIGPEIIVKTLAEMDPKTRAVSAVVGSMDIL
                     RRADKLLGTGLRFVDGLDAKSGEVPVANVLTKDQDKIRDGQVGAAGGEAAYRYVEAAV
                     QLALDGKIAVIVTAPLNKAAMHAAGHHFDGHTELLAHLTGAKSSFMLLAGDKLNAVHV
                     TTHTSLRNAAEKPTMERVVATIRAGHQHFRELGVANPRIAVAGLNPHCGEGGIFGREE
                     IDRIQPAVEAARKEGIDVKGPISGDTVFYRALKGEFDLVVAQYHDQGHIPTKLIAFDT
                     TVNVSLGLPIRRTSVDHGTAHDIAWTGKASNVNMKAAIAYARKMADKIAD"
     gene            1388..2602
                     /locus_tag="VE030_09550"
     CDS             1388..2602
                     /locus_tag="VE030_09550"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HZF23696.1"
                     /translation="MVPPPRVLIVADDLTGAMDSAGPFAGIGVETWVVAAPMQCDPAT
                     IARARVVSVNTDSRHLPAVHAATRVREIVRRLGPGAFDVVVKKIDSTLRGNVVAETLA
                     LLETSGRRWAVIAPAFPAQGRTVKSGVVHVNGVPLAETAFAGDALSPPPLAPLQQVFA
                     EARPGLGVAIAGAADDFSSQSDIRIWIVDGVTDADLAATVSAVQGRLGELLLAGSAGI
                     TKPLARACFGTAVSQAVPEKLTGTIVFAVGSRAVQSAEQVESLMQEPGTRILRAPNGR
                     LSQKEFPAARNVVLKATADDAGGEGDAQQVAVDMARNAIDVARRTQAQALVATGGDTA
                     IAILTVTGNPALQVLGDLMPGIPYARIRVDGTLLWLVTKAGGFGGRDTFRQIARRLRA
                     ETALTTVVERKG"
     gene            2820..4205
                     /locus_tag="VE030_09555"
     CDS             2820..4205
                     /locus_tag="VE030_09555"
                     /inference="COORDINATES: protein motif:HMM:NF039222.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HZF23697.1"
                     /translation="MDAIKHFLNPASIAVVGAGERPTSSGGAVLRNLVASGYKGRIVP
                     VNPKGGEILGLPAKASLKELDKPVDLVTVLVKPDSILEVVSEAAQTGHRNILVLPGGF
                     VEAGEAGRKRDDALRKFAAENGLTIGGPNCAGVINLLDPKAPFAATFFGAMPRGGGVA
                     MISQSGAIIEEVISASHALNIPLGAVVSVGNSMHLGVIEYLDQLGREDKCKAILLYLE
                     SFGDAARFAQVAREVAKIKPVVALIGGRTVPGREAAFRHTGSRPADEEATADFCNKNG
                     IVRVKSLRRLLLAGKAFGAFPRGIGRRVLLLSNSGGPGVLAADQATDEGLTLPELPPA
                     MAGKIRGFLPPEASVANPLDLLADAREDRFSGTLAAALEFGRDHFDAILIIHVIPFMV
                     EGGAVVEALAGLCKGAKTPILHSMMGTLEHKTEWFARLEDARVPVFNDSEEMCIAAGL
                     LARHRELNTVR"
     gene            4249..4887
                     /gene="maiA"
                     /locus_tag="VE030_09560"
     CDS             4249..4887
                     /gene="maiA"
                     /locus_tag="VE030_09560"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HZF23698.1"
                     /translation="MKLYGYFRSSASYRVRIALNMKGLEYEQVSVHLAKGRQYDPEFS
                     AISPQNLVPVLEHDGRFLYQSLAIIQYLDEKFPEPRLLPKDSPGRNRVCSLALISACE
                     IHPLNNTRVLAYLTDTLKVTDEQKNAWYRHWVTVGFAGLEKRLATEKETGKFCHGDTP
                     GFADITLVPQVANANRLKVDLTPFPTIRRINDECLKLEPFRKAMPANQPDAE"
BASE COUNT         1058 a         1563 c         1735 g          928 t
ORIGIN      
        1 atcggcaacg ccggcgggga actggcgacg gcgagggatg tacgcgctgc gctggcccct
       61 gaagaccagg aaaccctcta gggaacaatc tgctcagcgc tctataatct cggcctctca
      121 actggcagaa ccacaaggaa agcatgagct ccaggatcgg catcaccatg ggcgacccgt
      181 ctggtatcgg gccggaaatc atcgtcaaga cgctggccga gatggatccg aagacgcgcg
      241 cggtttccgc cgtggtgggc agcatggaca tcctccgacg tgccgacaag ctgctcggca
      301 ccgggctcag gttcgtcgac ggcctggacg cgaaaagcgg cgaggtgccg gttgccaacg
      361 tgctgaccaa agaccaggac aagattcgcg acggccaggt tggcgcggcc ggcggcgagg
      421 cggcctaccg ctacgtcgaa gcggccgtcc aactggcgct ggacggcaag attgccgtga
      481 tcgtgaccgc gccgctcaac aaggcggcca tgcatgcggc gggtcatcac ttcgacggac
      541 acaccgaact gcttgcgcac cttaccggcg cgaaatcatc gttcatgctg ctcgccgggg
      601 acaagttgaa cgccgttcac gtcaccacgc atacttcgct caggaacgcg gcggaaaagc
      661 cgaccatgga gcgcgtggtc gcgactatcc gcgccggaca tcagcatttt cgtgaactgg
      721 gcgtcgccaa tccgcgcatt gcggtggccg ggctgaatcc gcactgcggc gagggcggca
      781 ttttcggccg cgaggagatc gaccgcatcc agcccgccgt cgaagcggca agaaaggaag
      841 gcatcgacgt caaggggccg atctcggggg acaccgtgtt ctaccgcgcg ctcaagggcg
      901 aattcgacct ggtggtggcg cagtaccacg accaggggca tatccccacc aagctgattg
      961 ccttcgacac cacggtgaac gtcagccttg gcctgccgat ccgccgcact tcggtggacc
     1021 acggcaccgc gcacgacatc gcctggaccg gcaaggcgag caacgtcaac atgaaagcgg
     1081 ccatcgccta cgcgcgcaag atggccgaca agatcgcgga ttgatggcga gatgtcgtcg
     1141 agtcccatgc ttttggagaa aattcgggac gtaatattaa ccacgaaggc acgaaggcac
     1201 gaagacacga agaaaaccgg aaacagagga atttgcccga gcccttcttg catcagtgga
     1261 tatcggaaca ttccaacccc ttccaatgca tccaaacatg aataaaggaa atgcatctgc
     1321 ttctcttcgt gtcttcgtgg tgagattttc gttcccgggt cttccatcgg tcgatcgtga
     1381 gagctgaatg gttccccctc cccgggtact gatcgttgcg gacgacctga ccggggcgat
     1441 ggacagcgcc gggccttttg ccggaatcgg cgtcgaaacc tgggtggtag cggcgccgat
     1501 gcaatgcgat ccggcgacga tcgcccgggc gcgcgtggtt tcggtcaata ccgacagccg
     1561 tcatctgccg gcggtgcacg cggcaacccg ggtgcgcgaa atcgtgcgtc gcctcggtcc
     1621 cggggctttc gacgtcgtcg tcaagaaaat cgattccacg ctgcgcggca atgtggtggc
     1681 cgaaaccctg gcattgctgg agaccagcgg tcgccgctgg gcggtcattg cgccggcgtt
     1741 tccggcgcag gggcgcacgg tgaaaagcgg cgtggtccat gtcaacggcg tgccgcttgc
     1801 agagaccgct tttgccggcg acgcactgtc gccgccgccg cttgcgccgc tgcagcaggt
     1861 ctttgccgaa gcccggccgg ggctgggggt cgcgattgcc ggagccgcgg atgatttcag
     1921 cagccagtcg gatatccgca tctggattgt agacggtgtc accgacgcag acctggcggc
     1981 cacagtgagc gcggtgcagg gccggctagg cgaactgctg ctggcgggtt cggcgggaat
     2041 caccaaaccg ctggcccgcg cctgtttcgg cacggcggta tcgcaggcgg tcccggagaa
     2101 acttaccggc accatcgtct tcgcggtcgg atcgcgtgcg gtacaaagcg ccgagcaggt
     2161 cgaatccctg atgcaggaac cgggtacccg gatactgcgt gctcccaacg gacggctttc
     2221 gcaaaaggaa tttccagccg cgcgcaacgt ggtcctgaag gccactgccg acgacgcggg
     2281 cggggaaggc gacgcgcaac aggtggcggt ggacatggcg agaaacgcga tcgacgtggc
     2341 ccggcgtacg caggcgcagg cactggtcgc taccggcggc gacacggcaa tcgccatcct
     2401 cacggtgacc ggcaatcccg cgctgcaggt gctcggcgat ctgatgccgg gaattccgta
     2461 tgcacggatc cgcgtggacg gcacgctgct gtggctggtg actaaagcgg gcggattcgg
     2521 ggggcgcgac acgtttcgac agattgcgcg acggctgcgc gctgaaacag cgctgacgac
     2581 tgttgtggaa aggaaggggt aatgtatgga aaaaccgaag ttcttaccac gaagacacgg
     2641 aggcacgaag aaaagcaaat gcaattccgg aattgatgtg gaattacgat acgcggagtt
     2701 gaaatggtcc gatatctact gatgcaacca gattcgaacc gaaacatatg ttcccggttt
     2761 tcttcgtgcc ttcgtggtga gtattggatt cgaggaattt tgtcgttacg gataaaagat
     2821 tggatgccat caagcacttc ctgaatccgg cgagcatagc ggtggtgggc gcaggcgaac
     2881 ggccgaccag ttccggcggt gccgtgctgc gcaacctggt ggcatcaggc tacaaggggc
     2941 gcatcgtccc ggtgaatccg aagggcggcg aaattctcgg gctgcccgcg aaagcgtcgc
     3001 tgaaggagct cgacaagccg gtggacctgg tcaccgtgct ggtgaagccg gacagcattc
     3061 tcgaagtggt cagcgaggcc gcgcagaccg ggcacaggaa catcctcgtg cttcccggcg
     3121 gcttcgtcga ggcaggtgaa gcgggccgca agcgcgacga cgcgcttcgc aagtttgccg
     3181 cagagaatgg tctgacgatc ggtggtccga attgcgccgg cgtcatcaac ctgctcgatc
     3241 cgaaggcgcc cttcgccgca actttttttg gtgccatgcc caggggcggc ggcgtggcga
     3301 tgatttcgca gtcgggtgcg atcatcgagg aggtgatctc ggcgagccac gcgctcaaca
     3361 ttccgctggg agcggtggtg tcggtgggca acagcatgca cctcggcgtg atcgagtacc
     3421 tcgaccagct cggccgcgaa gacaagtgca aggcgatcct gctttatctc gaatccttcg
     3481 gcgatgcggc gcggttcgcc caggtcgcgc gcgaagtcgc gaaaatcaag ccggtcgtgg
     3541 cgctgatcgg cggccgtacg gtgcccgggc gggaagccgc gtttcgccat accggatcgc
     3601 ggcctgccga tgaggaggca accgcggact tctgcaataa aaacggcata gtccgggtca
     3661 aaagcctgcg gcgcctgctg ctcgcaggca aggccttcgg cgcctttccg cggggcatcg
     3721 gcaggcgtgt gctgctgctg tcgaattccg gcggccccgg cgtgctggcc gccgatcagg
     3781 ccacggacga gggactgacg ttgccggagt tgccgccggc gatggccggt aagattcgcg
     3841 gctttcttcc gccggaagcg tcggtggcga atccgctgga tctgctggcg gacgcgagag
     3901 aggaccggtt ttcgggcacg cttgccgccg ccctcgaatt cggccgtgat catttcgacg
     3961 ccatactgat cattcacgtg attccattca tggtggaggg cggagcggtg gtcgaagcgc
     4021 tggccggtct ttgcaaaggg gcgaagacac cgattctgca ttcgatgatg gggacgcttg
     4081 agcacaagac ggagtggttc gcgcgcctgg aagacgcgcg cgtgccggtg ttcaacgact
     4141 cggaagagat gtgtatcgcc gccgggctgc tggcgcgcca tcgcgaactg aatacagttc
     4201 gctgaagacg gccgacattc aatgccgcaa cgcgatgagg gggaaggaat gaagctctac
     4261 ggatacttca gaagctcggc atcgtaccgg gtgcggattg cgctcaacat gaagggactc
     4321 gaatacgagc aggtgagcgt tcatctggcg aagggcagac aatacgaccc ggagttctcg
     4381 gcgatcagtc cgcagaacct ggtgccggtg ctcgaacacg atggccgatt cttgtaccag
     4441 tccctggcga tcatccagta cctggatgag aagttccccg agccgcgatt gttgccgaag
     4501 gattccccgg gccggaaccg cgtgtgttct ctggcgctga tcagcgcctg cgaaatccat
     4561 ccgctgaaca atacgcgtgt gcttgcctat ctgaccgata cccttaaagt gactgacgaa
     4621 cagaagaatg cctggtaccg gcattgggtg acggtaggct tcgcgggcct cgagaaacgg
     4681 ctcgctacgg aaaaggaaac cggaaagttc tgccacggcg acaccccggg cttcgccgac
     4741 attacgctgg tgccgcaggt ggcaaacgcc aaccgcctca aagtggatct gacgccgttt
     4801 ccgaccatcc ggcgtatcaa cgacgagtgc ctcaagctcg agcctttcag gaaggcgatg
     4861 cccgccaacc agcccgacgc cgagtagcgc gttgcgtccg tctgccgact ggctgaccca
     4921 gaccgggcgg atctacatcg ggattcagca ccgcttttag acgcccgaca gtggggtcgc
     4981 cagtcgatgg cggccgatgt cgcctacctc cctacgattc cgcgcgagta gactagtaac
     5041 ggagtcgtag cagtcaggcc gggtcaccgg ccgcacaaag gaaggggcaa tgatcgccgc
     5101 aaatcatcag acccttggcc cgctctgcac ggcatccggc cgctgcttga tggatggaat
     5161 gccgcttcaa ttgaaactgc tccgcccgga gcacgccaga ctactttttt gccggcaagt
     5221 tcgacgacct gaggtcacag gtcgatgatg cttgagaaag gcggtaagca tggcaacgcc
     5281 taca
//
LOCUS       DASQOP010000237         3566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1725278, whole genome shotgun sequence.
ACCESSION   DASQOP010000237 DASQOP010000000
VERSION     DASQOP010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1725278"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..127)
                     /locus_tag="VE030_09565"
     CDS             complement(<1..127)
                     /locus_tag="VE030_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004550866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HZF23699.1"
                     /translation="MRTEYCGLVSTRHLDHTVTLYGWVHRRRDHGGVIFIDLRDRE"
     gene            complement(206..820)
                     /locus_tag="VE030_09570"
     CDS             complement(206..820)
                     /locus_tag="VE030_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HZF23700.1"
                     /translation="MKKYLITGLLIWVPLVITVWVLSLLVDTLDLTLLLLPTSWQPRS
                     WLGFHIPGLGVLLTLAVVFVTGVFAANIIGQRLVMYWEGLLARIPIVKSLYSSVKQVS
                     DTLFSSSGVAFRKALLVQYPRQGSWTIAFLTGQPGGDVANYLKGEYVSVYVPTTPNPT
                     SGFFLMMPTADVVELDISVDAALKYIISMGVVPPEDKSATSDAA"
     gene            complement(820..1203)
                     /locus_tag="VE030_09575"
     CDS             complement(820..1203)
                     /locus_tag="VE030_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZF23701.1"
                     /translation="MPIYEYRCSSCGHEKEVLQKLSDPPLTECPACGKSTLRKLVSAA
                     GFHLKGSGWYATDFKGGVKPKEGSQEAQDKSASKDKQEGAKSKEQHAAKEDAKATKEP
                     TPVTTTTTATTTKAPLPATTAGKAD"
     gene            1354..2106
                     /gene="ugpQ"
                     /locus_tag="VE030_09580"
     CDS             1354..2106
                     /gene="ugpQ"
                     /locus_tag="VE030_09580"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017333574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HZF23702.1"
                     /translation="MAQSRSVWPYPRIIAHRGGGVLAPENTLAALELARNLGFEGVEF
                     DVKLSEDGTPILMHDDTLDRTTDGHGPVADYAYQEIAELDAGAWFGNEFIGEPVPAFA
                     AAAALCQQAGLWANVEIKPCAGRERETGEVVGRMAKLLWSGANLPPLLSSFSAIALEA
                     AAVEAPELPRALLVVEAPVNWQAQMQRLQCVSLHASHRHLNAALVKAVHDAGYGVLTY
                     TVNDSELALELLEWGVDALVTDQLDVITPHFA"
     gene            2261..3103
                     /locus_tag="VE030_09585"
     CDS             2261..3103
                     /locus_tag="VE030_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23703.1"
                     /translation="MISNSKTNVTVVQEELHRGAVLQKALDSNSNSMRSIPLAAALAV
                     ACVSAWSALPDEIQVYTDDLEAPGEHGMELHINTTPKGRSTPDYPGEVVPHHGLRITP
                     ELSFGLAQHWDAGIYLPFVRSGEGTEYFAGPRLRLKWLPVRPAEGNAGYFAGINWEVS
                     FVERKFEQARRTAEIRPIVGYRSADWLLAFNPNIDTDLAGSQKGELIFAPAFKAARNV
                     CSQTALGVEYYAEFGRLSNFAPQSQQSHTLFLVVDTHRFNFGIGRGLNGASDRWTVKG
                     IISF"
     gene            complement(3244..>3566)
                     /locus_tag="VE030_09590"
     CDS             complement(3244..>3566)
                     /locus_tag="VE030_09590"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HZF23704.1"
                     /translation="IYLSLTPDPPDLGMPEGLKIGHLLAYGWLMIWFAQIYRATGRRL
                     LFAAAFCALGIVLEYLQGMTDYRGFEYSDMLINSTGVVLGLALARTPLQDGLRTLEAI
                     LRAR"
BASE COUNT          714 a         1103 c         1096 g          653 t
ORIGIN      
        1 cttcccggtc cctgaggtcg atgaagatca cgccgccgtg gtcgcggcgc cggtgcaccc
       61 agccgtagag cgtgacggtg tggtcgagat gccgggtgga aacgagtccg cagtattcgg
      121 tgcgcataag ttcaggatcg gagattgaga atcggaggtc gcatgaggcc gcgcgcattt
      181 gcacgcacga aaaagggcaa aacggtcacg ccgcgtcgct ggtcgcgctc ttgtcttccg
      241 gcggcaccac gcccatcgag atgatgtact tgagcgcggc atcgacgctg atatcgagtt
      301 cgacgacatc cgcggtaggc atcatcagga agaacccgga agtcgggttg ggtgtggtcg
      361 gcacgtacac gcttacgtat tcgcctttca gatagtttgc tacatcgccg cccggctgcc
      421 ctgtgagaaa agcgatggtc catgaaccct ggcgtggata ctgcaccagc agcgccttgc
      481 gaaatgccac cccggacgag gaaaacagcg tgtccgaaac ctgcttgacg ctggaataaa
      541 gcgacttgac gatcgggatg cgggcgagca gaccttccca gtacatcacc aggcgctggc
      601 cgatgatgtt ggcggcgaac accccggtca cgaacacgac tgccagcgtc aacagcacgc
      661 ccaggccagg tatgtggaag cctagccacg atcgtggctg ccaggaagtc ggaagcagca
      721 gcagcgtcag gtcgagggtg tcgaccagaa gactcagcac ccacactgta atgaccagcg
      781 ggacccagat cagcagcccg gtgatgaggt attttttcac tagtccgcct taccagcggt
      841 cgtggcgggc agcggcgcct tcgtggtagt cgcggtagtc gtggtcgtaa caggggtcgg
      901 ctctttcgtg gccttggcgt cttccttggc cgcgtgctgt tccttggact tggccccctc
      961 ctgcttgtcc ttgctcgcgc tcttgtcctg cgcctcctgc gagccttcct tgggcttgac
     1021 accacccttg aagtcggtcg cgtaccagcc gctgcctttc aagtggaaac ccgctgcact
     1081 caccagtttc cgcagcgtcg acttgccgca ggcggggcac tcggtcaggg gcgggtccga
     1141 cagcttctgc agaacctctt tttcgtgccc gcaactgcta catcgatatt cgtagatggg
     1201 catggtatcc ctcgcaaaaa caacggaaat tataccgtat caagcacatg caactgctat
     1261 gcagatcagg gcttgcggcg cggatatcaa ggcgggacgg ggtctggcgg gcaatcgcta
     1321 taatcaggca ccccgaatct cggcaacgac aacgtggccc aatcccgatc cgtctggccc
     1381 tatccccgca tcatcgccca ccgcggcggc ggcgtactcg cccccgagaa cacgctcgcg
     1441 gcgctggaac tcgcacgcaa tctcgggttc gagggagtgg agttcgacgt caaactcagc
     1501 gaggacggca cgcccatcct gatgcacgat gacacgttgg atcgcaccac cgatggccac
     1561 ggtcctgtcg cggactatgc gtaccaggag atcgccgaac tcgatgcggg cgcatggttc
     1621 ggcaacgaat tcatcggcga accggtgcca gctttcgccg ccgcggcggc actgtgccag
     1681 caggccgggc tgtgggccaa cgtcgagatc aagccctgtg ccggacgcga gcgcgaaacc
     1741 ggagaggtcg tgggccggat ggccaaactg ttgtggagtg gcgccaatct accgccgctg
     1801 ctgtcgtcct tctccgcgat cgcactggaa gccgcggcag tcgaggcacc cgaactgccg
     1861 cgcgcgctgc tggtagtcga ggcgccggtc aactggcagg cccagatgca gcggctgcag
     1921 tgcgtgtcgc tgcacgccag ccaccggcac ctgaacgccg cattggtcaa agctgtgcac
     1981 gatgccggct acggcgtgct gacctacacc gtcaacgatt cggaactggc gctggaactg
     2041 ctggaatggg gagtggacgc gctggtgacg gatcagctgg acgtcatcac gccccacttc
     2101 gcttgagtcg aaacgttttc accgcaaagg cgtaaagaac gcaaaggtac gcgaaggaaa
     2161 accgaagcgc gggaacgaaa ttcggccccg attgcttgtc ccttagctgc gctgtaacgt
     2221 attatgcgca cgccccccaa agggagggat cgaagttgaa atgatctcca actccaagac
     2281 caatgtcacc gtcgtacaag aagaactaca tcgcggtgcg gtgcttcaga aagcgttgga
     2341 ctcgaacagc aatagcatgc gctccatccc tctcgcggcg gcgctcgcgg tcgcctgcgt
     2401 gtctgcatgg tcggcgctcc ctgacgaaat ccaggtttac accgacgatc ttgaagcccc
     2461 gggcgagcac ggcatggagt tgcacatcaa caccacgccg aagggccgct cgacgcccga
     2521 ctacccggga gaggtcgttc cgcaccatgg actgcgcatc acaccggaac tttcgttcgg
     2581 cctcgcacag cactgggatg ccggcatcta tctgccgttc gtcagaagcg gcgaggggac
     2641 ggagtacttc gccggcccgc gcctccggct gaagtggctt ccggtgcgcc ctgccgaggg
     2701 caatgccggt tacttcgctg gcatcaactg ggaagtttct ttcgtcgagc gcaagttcga
     2761 gcaggcgcgc cgcacggccg aaatccggcc catcgtgggc taccgcagtg ccgactggct
     2821 gctggcgttc aatcccaaca ttgacaccga tctcgccggt tcgcagaaag gagagctgat
     2881 attcgcgccg gcgttcaagg cagcgcgcaa cgtgtgtagc cagacggccc tgggcgtgga
     2941 gtactacgcc gagttcggcc ggctgtcgaa tttcgccccg cagagccagc agtcgcacac
     3001 gctgttcctg gtagtcgata cgcatcgttt caatttcggc atcgggcgtg gcctgaacgg
     3061 cgccagcgac cgctggaccg tcaagggcat tatctcgttc tagggttgtg aaatctcccg
     3121 cccgccatat cgcgcacggc gcgttccggc gggcaaataa ctactgtgga attttccatg
     3181 gcccatgcga ggcgtcgatt gcaattccgg tgctggccga aaccggtacg gcggcacccg
     3241 tgctcaccgt gcacgcagta tcgcttcaag cgtccgcagg ccgtcctgca acggggtgcg
     3301 cgccagggcc agaccaagta ccacgccggt cgagttgatg agcatgtccg agtattcgaa
     3361 accacggtag tcggtcattc cctgcagata ctcgagcacg atcccaagcg cacaaaatgc
     3421 ggccgcaaac aacaaccgtc ggccggtcgc gcggtaaatt tgcgcgaacc agatcatcag
     3481 ccagccatag gcgagcaaat ggccgatttt caagccttca ggcatgccaa ggtcgggcgg
     3541 atctggtgta agcgacagat agataa
//
LOCUS       DASQOP010000238         4987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1523800, whole genome shotgun sequence.
ACCESSION   DASQOP010000238 DASQOP010000000
VERSION     DASQOP010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523800"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1681
                     /locus_tag="VE030_09595"
     CDS             <1..1681
                     /locus_tag="VE030_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HZF23705.1"
                     /translation="REMRTALTTTSLSGTKVPKVALPKFEDDGETLKWLMRENLPGYF
                     PYTAGVFPFKRENEDPTRMFAGEGDPFRTNARFKFLSRDMPAKRLSTAFDSVTLYGFD
                     PHERPDIYGKVGNSGVSIATLDDMKVLYSGFDLCDPATSVSMTINGPAPTILAMFANT
                     AIDQQIEKFAAQHGRQPNEQETRKIRAWALENVRGTVQADILKEDQGQNTCIFSTEFS
                     LKVMGDIQEFFVRNKVRNFYSVSISGYHIAEAGANPISQLAFTLSNGFTFVEAYLARG
                     MDIDDFAPNLSFFFSNGMDPEYTVLGRVARRIWATTMRNKYGANERSQKLKYHVQTSG
                     RSLHAQEIAFNDIRTTLQALIAIYDNCNSLHTNAYDEAITTPTAESVRRALAIQLIIN
                     REWGLAKNENPNQGSFIIDELTDLVEEAVLQEFERISERGGVLGAMETGYQRGKIQEE
                     SLYYEHRKHDGSYAIIGVNTFRNPDAVEAGGSIPLQRSTEEEKQGQIRRLREFQKRND
                     DEAPAALEKLKQTVIDNGNVFAELVNAVRFCSLGQITDALFEVGGQYRRSM"
     gene            1681..1818
                     /locus_tag="VE030_09600"
     CDS             1681..1818
                     /locus_tag="VE030_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23706.1"
                     /translation="MRAWPPRVLNLKEEYGVADFQLDHLNMPARDPGDNCLEIYCKSD
                     T"
     gene            1902..3872
                     /locus_tag="VE030_09605"
     CDS             1902..3872
                     /locus_tag="VE030_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008120182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZF23707.1"
                     /translation="MKHPDTGGRGVADTFPALLLHHARLRGEWTAIREKDLGIWQSWS
                     WSQVAEEVRALACGLAAQGFKRGMNLAIIGDNRPRLYWSFAAAQSLGGVPVPLYQDSV
                     AQEMLYVLENAEIEYAIVEDQEQVDKLLEILPQYPKLKHIYYDDPRGLRNYRQPQLMA
                     YDRLREIGLEHHRQHPDLFHGAVAQVRPEDVCVMLYTSGTTGKPKGVCHTHAGFIAAA
                     RGDMEFDGFTPDDEVLSYLPMAWVGDHLFSYGQAMVAGFTVNCPESSETVMTDMREIG
                     PTYYFGPPRVFENLLTTVMIRIDDASRVKRHLFHSFMKVARRCGAEIMDGKPVSLSDR
                     LLYWLGDLLIYGPLRNVLGMSRIRVAYTGGAAIGPDLYRFYRSIGINLKQLYGQTETS
                     VYVCKQATGAAKLDSVGRPLPGVEIKIAGNGEVLVKTPGMLKEYYKRPDATAESISPD
                     GYFLTGDAGFLDADGDLKIIDRARDVGKLADGSLFAPQFVENKLKFFPCIKEAVCFGD
                     QREMVCAFINIDMQAVGNWAEKRNLPYSGYIDLAGRPEVYDLIQKCVEQINADLAKEP
                     HLAGSQIRRFLVLHKELDPDEDELTRTRKVRRSFVAQKYAVLVDALFGSKASQFIRTQ
                     VRFEDGRTGNIEADVKIRDAATYSAAALRINA"
     gene            3923..4672
                     /locus_tag="VE030_09610"
     CDS             3923..4672
                     /locus_tag="VE030_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZF23708.1"
                     /translation="MENISLTFGGVKALNEISFDVCEHEIRAIIGPNGAGKSSLLNVI
                     NGVYHPNEGTITYKGNTRRQMRPHEAASQGIARTFQNIALFKGMTVLDNIMTGRTLKM
                     HAGFLSQCLYFGSAQREELEHREKVEQIIDFLQIESIRKTPVGRLAYGLQKRVELGRA
                     LAAEPELLLLDEPMAGMNVEEKQDMSRFILDVNDEFGTTIVLIEHDMGVVMDLSDRVV
                     VLDYGRNIGDGTPDEVRGNQAVIDAYLGVAH"
     gene            4857..>4987
                     /locus_tag="VE030_09615"
     CDS             4857..>4987
                     /locus_tag="VE030_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007246480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZF23709.1"
                     /translation="MSFFFEVLIGGLMAGVLYSLVALGFVLIFKASGVFNFAQGAMVL
                     "
BASE COUNT         1051 a         1535 c         1530 g          871 t
ORIGIN      
        1 tcgcgagatg cgcacggcgc tgaccaccac gtcactgtcc ggaaccaagg tgccgaaagt
       61 ggcgctaccg aagttcgagg acgatggcga gacgctgaag tggctgatgc gggagaacct
      121 gcccggctat ttcccgtaca cggcgggggt gttcccgttc aagcgcgaga acgaagaccc
      181 gacccggatg tttgccggcg agggcgatcc gttccgcacc aacgcacgct tcaagtttct
      241 cagccgcgac atgccggcga agcggctgtc gaccgcgttc gattcggtga cgctatacgg
      301 cttcgacccg cacgaacgtc cggacatcta cggcaaggtc ggtaactccg gggtgtcaat
      361 cgcgacgctg gacgacatga aggtgctgta ctccggcttc gacctgtgcg atcccgcgac
      421 ctcggtgtcg atgaccatca atggaccggc cccgaccatc ctggcgatgt tcgcaaacac
      481 cgccatcgac cagcagatcg agaaattcgc cgcgcagcac ggccgccagc cgaacgaaca
      541 agagaccagg aagatccgcg cctgggcgct ggaaaacgtg cgcggcacgg tgcaggccga
      601 catcctcaag gaagaccagg gccagaatac ctgcattttc tccaccgaat tctcgctcaa
      661 ggtgatgggc gacatccagg agttcttcgt acgcaacaag gttcgcaatt tctattcggt
      721 gtcgatctcc ggctatcaca tcgccgaagc cggcgccaac ccgatctcgc agcttgcctt
      781 caccttgtcc aacggcttca ccttcgtcga ggcttatctc gcgcgcggca tggacatcga
      841 cgacttcgcg ccgaacctgt cgttcttctt ctccaacggc atggacccgg aatacacggt
      901 gctcggacgc gtggcgcggc gcatctgggc aaccacgatg aggaacaagt acggcgccaa
      961 cgagcgcagc cagaagctga agtaccacgt ccagacttcg ggccgttcgc tgcacgcgca
     1021 ggagattgcg ttcaacgaca tccgcaccac gctgcaggcg ctgatcgcga tctacgacaa
     1081 ctgcaactcg ctgcacacca acgcctacga cgaggccatc accacgccga ccgccgaatc
     1141 ggtgcggcgc gcactcgcga tccaactcat aatcaaccga gagtggggcc tggcgaaaaa
     1201 cgagaacccg aaccagggct cgttcatcat cgacgaactg accgatcttg tcgaagaagc
     1261 agtactgcag gagttcgagc gcatctccga gcgcggcggc gtgctcggcg caatggaaac
     1321 cggttaccag cgcggcaaga tccaggaaga atcgctgtac tacgagcaca ggaagcacga
     1381 cggctcctac gcgatcatcg gcgtgaacac cttccgtaac ccggacgcgg tcgaggccgg
     1441 cggcagcatc ccgctgcagc gttcgactga ggaagagaag caagggcaga tacgccggtt
     1501 gcgcgagttc cagaagcgca acgacgacga ggctccggct gcgctggaga aactgaagca
     1561 gaccgtgatc gacaacggca acgtgtttgc cgaactggtg aacgccgtgc gcttctgctc
     1621 gctcggccag atcaccgacg cattgttcga agtcggcggg cagtaccgca gaagcatgta
     1681 gtgcgagcct ggccgccgcg tgttttgaat ctcaaggaag aatatggggt ggccgatttc
     1741 cagctcgatc acctgaacat gccggcgcgc gacccgggag acaactgcct cgagatctac
     1801 tgcaagtcgg acacctgagc gcgaccgcgg cacgaaggtg ataaaaagca tccggaccgg
     1861 tcatgagccg gcccgggata acgaacaggg aggagcgcgc cttgaagcac ccggacactg
     1921 gcggccgcgg ggtcgcggat accttccccg ctttactcct gcatcacgct cgcctgcgcg
     1981 gggagtggac cgcgatccgc gagaaggacc tcggtatctg gcagagctgg agctggtcgc
     2041 aagtcgccga ggaggtccgg gcgctcgcgt gcggcctggc cgcccagggt ttcaagcgcg
     2101 ggatgaacct cgccatcatc ggcgacaacc ggccgcgtct gtactggtcg ttcgccgccg
     2161 cgcaatccct cggtggcgtg ccggttccgc tgtaccagga ttcggtcgct caggagatgc
     2221 tgtacgtgct cgagaacgcc gaaatcgagt acgcgatcgt cgaggaccag gaacaggtcg
     2281 acaagctgct cgaaatcctg ccgcaatacc cgaaactcaa gcacatctac tacgacgatc
     2341 cgcgcgggct gcgcaactat cgccagccgc agttgatggc ctacgaccgg ctgcgcgaaa
     2401 tcggtctcga gcaccaccgc cagcatccgg atctcttcca cggcgccgtt gcgcaggtcc
     2461 gccctgagga cgtctgcgtg atgctctaca cttcggggac caccggcaag cccaaaggcg
     2521 tgtgtcacac acacgctgga ttcatcgccg cggcacgcgg cgacatggag ttcgacggct
     2581 tcacgcctga cgatgaagtg ctttcttacc tgccgatggc ctgggtcgga gaccatctgt
     2641 tctcctacgg acaggcaatg gtggcaggct tcaccgtcaa ttgcccggag tcgtccgaaa
     2701 cggtgatgac cgacatgcgc gagatcggtc cgacgtatta tttcgggccg ccccgggttt
     2761 tcgagaactt gctgacgacg gtgatgattc gcatcgacga cgccagccgg gtcaagcgcc
     2821 acctgttcca cagcttcatg aaggtggcgc gccgctgcgg ggcggagatc atggacggca
     2881 agccggtttc gctgtcggac cgactgttgt attggcttgg cgatctgttg atctatgggc
     2941 cgctacgcaa cgtgctgggt atgagccgta tccgggtggc gtataccggc ggcgccgcga
     3001 tcgggccgga cctgtatcgc ttctaccgct cgatcggcat caacctcaag caactgtatg
     3061 gccagaccga gaccagcgtc tacgtttgta agcaggcgac tggcgctgcc aaactcgatt
     3121 cggtcggccg gccgttgccg ggagtggaga tcaagatagc cggcaacggc gaagtgctgg
     3181 taaagacgcc cggcatgctc aaggagtact acaagcgccc ggatgcgacc gcggaatcca
     3241 tatcgcccga cggatatttc ctgaccgggg atgccggctt cctggacgcc gacggcgacc
     3301 tgaagatcat cgaccgcgcc agagatgtcg gcaagctcgc ggacggctcg ctgttcgcgc
     3361 cgcaattcgt cgagaacaag ctcaaatttt tcccctgcat caaggaagcg gtctgtttcg
     3421 gcgaccagcg cgagatggtc tgcgccttca tcaacatcga catgcaggcg gtgggcaatt
     3481 gggccgagaa gcgcaacctg ccctatagcg gatatatcga tctcgccggc aggcccgagg
     3541 tctacgacct gatccagaaa tgcgtcgagc agatcaatgc cgaccttgcc aaggagccgc
     3601 atctggccgg ttcgcagatc cgtcgcttcc tggtgttgca caaagagctc gatccggacg
     3661 aagacgagct cacgcgcacc cgcaaggtgc gccgcagctt tgtcgcgcaa aagtacgccg
     3721 tgctggtgga tgcgttgttc ggcagcaagg cctcgcagtt catccgcacg caggtcaggt
     3781 tcgaggacgg ccgcaccggc aacatcgagg ccgatgtaaa aatccgcgac gccgccactt
     3841 actctgccgc ggcgctacgc atcaacgcct gaacgtgaac gcgaacccag ccgatcgccg
     3901 tatcggcgac gttatcctga aaatggagaa catctcgctc accttcggcg gcgtcaaggc
     3961 gctcaacgag atcagtttcg acgtgtgcga acacgagatc cgcgccatta tcgggcccaa
     4021 cggcgccggc aagagttcgc tgctcaacgt catcaacggc gtgtaccacc ctaacgaggg
     4081 taccatcacc tacaagggca acacgcgccg gcagatgcgc ccgcacgagg cggcgagcca
     4141 ggggatcgcg cgcacctttc agaacatcgc gctattcaag gggatgacgg tgctcgacaa
     4201 catcatgacc gggcgaaccc tgaagatgca cgccggcttc ctgtctcagt gcctgtattt
     4261 cggcagcgcg cagcgcgagg aattggagca ccgcgagaaa gtggagcaga tcatcgattt
     4321 tctgcagatc gagtcgattc gcaagacgcc ggtggggcgg ctcgcctacg gcctgcagaa
     4381 acgggtcgaa ctcggccgcg cgctggcagc ggaaccggag ctgctgctgc tcgacgaacc
     4441 gatggccggc atgaatgtag aggaaaagca ggacatgagc cgcttcatcc tcgacgtcaa
     4501 cgacgaattc ggcaccacga tcgtgctcat cgagcacgac atgggggtag tgatggatct
     4561 gtccgaccgc gtggtggttc tcgactacgg ccgcaacatc ggcgacggca cgccggacga
     4621 ggttcgcggc aaccaggccg tcatcgatgc ctatctgggc gtcgcccact agaaattttt
     4681 caccacagag acgcaaggga cgcaaaggaa atcaatgaaa aggcggaatg gcgatgaaaa
     4741 tacagctagc cgcacgactg ttgcgccgac caaaataccg cgtgccgggg aatttcattc
     4801 ggatctgaat ttctctgcgt tctctgcgcc tctgcggtga gatcgggaca tagagaatgt
     4861 cgtttttctt cgaagtgctg atcggcgggc tgatggcggg agttctctac tcgctggtgg
     4921 ccctgggctt tgtgctgatc ttcaaggcat cgggcgtgtt caatttcgcc cagggtgcca
     4981 tggtgct
//
LOCUS       DASQOP010000239         2742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_114421, whole genome shotgun sequence.
ACCESSION   DASQOP010000239 DASQOP010000000
VERSION     DASQOP010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114421"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VE030_09620"
     CDS             complement(<1..215)
                     /locus_tag="VE030_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010107039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZF23710.1"
                     /translation="MNRGSEARRMVRHFRSGVLATQSLKFPGYPYASALPFCTDQQGR
                     PVVLVSHLAEHTQNADHDRRVSFLVAPS"
     gene            complement(212..805)
                     /gene="wrbA"
                     /locus_tag="VE030_09625"
     CDS             complement(212..805)
                     /gene="wrbA"
                     /locus_tag="VE030_09625"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HZF23711.1"
                     /translation="MQEILVLYYSHTGAVKQMAQFIARGVEKVPGMRARLRTVPKVST
                     VSEATEAEIPDSGAPYVELRDLEDCAGLALGSPTRFGNMAAALKYFLDGTGALWLKGA
                     MAGKPAAVFTSTASLHGGQEATLLSMMLPLIHQGMLIVGLPYTEADLMTTRGGGTPYG
                     ASHLAGANADQPIGEGERRLCMALGTRLAEVALKLSK"
     gene            863..2125
                     /locus_tag="VE030_09630"
     CDS             863..2125
                     /locus_tag="VE030_09630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HZF23712.1"
                     /translation="MKLRLRSLLDIVRQFKLLANFVVLVYRRFKEERCFQLCGSLTFT
                     TLLALVPLVTIMLTVVTAFPVFDDIVQTLRSFVTANMVPEASSSLITVYVQQFSDNAA
                     RLTALGIVLLAVTSIMLMLTIDRAFNTIWRVKRPRPLIQRVLIYWSVLTIGPLLVGGS
                     LSLTSWLVTQSMGLGKQAPELTIAVLRLVPLMLTSVAFGFLYRTVPNRQVTVLDAAVG
                     GIIAAFAFEAMKIGFGHFVARIATYKLVYGTFASLPIFLMWIYLSWVVVVFASVITAV
                     LPYWRTGGVLLKQPPGAQFVEAVEILKMLYRAYADGRVLNLQQLRIAVKLSWEDAEAI
                     LDKLVAAGWVAKLQGNGWVLARDASAIRVGDVFQMFVFGREAADVSEDSALRRLVSSI
                     AASVDGELDFSLKELLASSGGQRAARIA"
     gene            2130..2594
                     /locus_tag="VE030_09635"
     CDS             2130..2594
                     /locus_tag="VE030_09635"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZF23713.1"
                     /translation="MLRQGLFRFAGVLLLAFAASAALAADFKLTDSDGKVHRLADYRG
                     KWVIVNFWATWCPPCLEEIPDLVAITGARQDVRVFGVAMEFQDPKQVLQFAEGMFVNY
                     PIVLGDRKTADQVGKVMGLPTTFIYDPQGKLAVRHVGKITRKQIERLLDRKA"
BASE COUNT          462 a          833 c          890 g          557 t
ORIGIN      
        1 gagggcgcaa ccaggaagct cacgcgccgg tcgtggtcgg cgttctgggt gtgctcggca
       61 aggtggctga ccagcaccac tggacgcccc tgctggtcgg tgcagaaggg cagggcggag
      121 gcatagggat agccgggaaa cttcaacgac tgggtggcaa gcacgccgct gcgaaaatgg
      181 cgcaccatcc tgcgcgcctc cgaaccacga ttcatttcga cagcttgagc gctacttcgg
      241 ccagccgcgt tcccagcgcc atacacagcc gccgctcgcc ttcaccgatc ggctggtcgg
      301 cattggcgcc ggcgagatga cttgcaccgt aaggcgtgcc gccgccgcgg gtggtcataa
      361 gatcggcttc ggtatagggc agtccgacga tcagcatgcc ctgatgaatc agcggcagca
      421 tcatcgacag cagcgtggct tcctggccgc cgtgcagcga cgcggtggag gtgaacaccg
      481 cggcaggttt gccggccatc gcgcccttga gccagagcgc accggtgccg tcgaggaagt
      541 atttgagcgc cgccgccatg ttgccgaaac gagtggggct gccgagcgcc agaccggcgc
      601 aatcctccag gtccctgagt tcgacgtaag gggcgccgga atcgggaatc tccgcttcgg
      661 tggcttccga gaccgtcgag accttgggca cggttcgcag ccttgcccgc atgcccggta
      721 ccttttcgac cccgcgcgcg ataaactgcg ccatctgctt taccgcaccg gtgtggctgt
      781 agtacagcac caggatttcc tgcatcgcta ttttcccgcc ggagaagccc gtattatatc
      841 ggcgaaacca cacccgaccc cgatgaaact ccggctcaga tcgctcctgg acatcgtccg
      901 ccagttcaag ctgctggcga atttcgtcgt gctcgtgtac cggcggttca aggaggaacg
      961 ctgcttccaa ctgtgcggca gcctgacctt caccacgctg ctggcgttgg tgccgctggt
     1021 caccatcatg ctcacggtgg tgaccgcgtt tccggttttc gacgacatcg tccagacgct
     1081 gcgcagcttc gttaccgcca acatggttcc ggaggcgtcg tcgagtttga tcaccgtgta
     1141 cgtgcagcag ttttccgaca acgccgcgcg actcactgcg ctgggtatcg tattgctggc
     1201 cgtgacttcg atcatgctga tgctcacgat cgaccgcgcc ttcaacacca tctggcgggt
     1261 gaagcggccg cggccgctga tccagcgggt gctgatctac tggtcggtgc tgaccatcgg
     1321 cccgctcctg gtgggcggca gcctgtcgct tacttcctgg ctggtgactc aatcgatggg
     1381 gctgggaaag caggcgcccg agctgacgat cgcggtgctg aggctggtgc cgctgatgct
     1441 cacgtcggtg gcgttcggat tcctgtaccg tacagtgcca aaccgtcagg tcaccgtact
     1501 cgatgccgcg gtcggcggca tcattgcggc ctttgccttc gaagcaatga aaatcggctt
     1561 cggccatttc gtcgccagaa tcgctaccta taagctggtg tacggcacgt ttgccagcct
     1621 gccgatcttt ctaatgtgga tctacctgtc ctgggtggtt gtcgtgttcg catcggttat
     1681 caccgcggtg ctgccctatt ggcgtaccgg cggcgtgctg ctcaagcagc cgcctggcgc
     1741 gcaattcgtg gaggctgtgg agattctcaa gatgctctac cgcgcctacg ccgacgggcg
     1801 ggttctgaac ctgcagcaat tgcgcatagc ggtaaagctt tcatgggagg acgccgaggc
     1861 cattctcgac aaactggtgg cggcgggttg ggtggcgaaa cttcagggca acggctgggt
     1921 tctcgcccgg gacgccagtg caatccgcgt cggcgacgta ttccagatgt ttgtgtttgg
     1981 gcgcgaagcc gccgacgtca gcgaagactc cgccctccgg cgactggtgt ccagcatcgc
     2041 ggccagcgtg gacggtgaat tggacttcag cctgaaagaa ttgctggcga gcagtggtgg
     2101 ccagcgtgcc gcgagaatcg cctgagggaa tgttgcggca gggcctgttt cgtttcgccg
     2161 gcgtgctgct gcttgcgttc gccgcgtcgg cggccttggc agcggacttc aagctgaccg
     2221 actcggacgg caaggtgcat cgcctcgccg attaccgcgg caaatgggtg atcgtgaatt
     2281 tctgggcgac ctggtgtccg ccctgcctgg aagagattcc ggatctggtg gcgatcaccg
     2341 gggcgcgcca ggacgtgcgg gtgttcggcg tggcgatgga attccaggat ccgaagcagg
     2401 tgctgcagtt cgccgaaggc atgttcgtca attacccgat cgtgctcggc gacaggaaaa
     2461 ctgcggatca ggtcggcaag gtcatggggt tgcctacgac gtttatttac gacccgcagg
     2521 gcaaactggc ggttcgccac gtcggcaaga tcacccgcaa gcagatcgag cgcctgctcg
     2581 atcgcaaagc ctgaggcctt ggccacaggg gacatccacc tcaccccatc cctctccccc
     2641 gatagcgggc ggagagggtg aaggtcacgt tgctttcggg caccctgtcc agcttgctgg
     2701 cgagggcggg ggggtgagga gtttcttctc cgtgtttctc tg
//
LOCUS       DASQOP010000240         4393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1122707, whole genome shotgun sequence.
ACCESSION   DASQOP010000240 DASQOP010000000
VERSION     DASQOP010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122707"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VE030_09640"
     CDS             complement(<1..675)
                     /locus_tag="VE030_09640"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZF23714.1"
                     /translation="MPSPLLNVSWDHCLLDPAPDRAAELALRRETGAAPGWIRYFLSC
                     PWLPRAAIRLGIDNGLLVHVDFPTVDLIALVVSQENSCRYCYAITRMALRVLGMSEER
                     MQQLEQRLASGELEPKAAAAVNFGRRMTRATPLVSPQDLEPLRAAGYSDAEIREIAFT
                     AACIAFFNRISTIPALPPHSWEQLADRWFMRLFRPLFARTVRGWRKRGQPVSFARPPQ
                     GPFAGLL"
     gene            complement(697..2085)
                     /locus_tag="VE030_09645"
     CDS             complement(697..2085)
                     /locus_tag="VE030_09645"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HZF23715.1"
                     /translation="MIDGPVVMPSGGMHATLEQRRGQSMEMMAWVALAIFVLSILAVI
                     SNVVDGTIAALIGVAVMVWLGVMTEVEAFQLVDWNVMAILISIWMIAGYFGKTGVPSW
                     LSVQALRLSGGRPGLLVMILSMLAGVISMFVDNVVVILMMAPVALPLARALRLPITPL
                     VLMIGFSANFMGSALLLGDLPPQMLHSISGAEFMEFIWSQGRPSSFPILMATFIVTLA
                     AMYAYGFRTHSRRAVDIGSLGIETHIPNRLFACVVVGWFLLTVLAMAFREMLGVKLGF
                     IALTGAITLVLALELLGERVKAPALEKILGEFDWRAIFFYVALFALVGGLEKMNLIDA
                     LAHQIRPVFSENFALGATLLYWTTVPIVGIVEHDAYILTFLYTIRDLQSEGVPPWPLW
                     WMLLWSGTLGSNLTVAGAPALFAALNICEREEGRKVSLREFLSWSVPFTLVAAGVCYL
                     FGMLIWVLPYIR"
     gene            complement(2147..2869)
                     /locus_tag="VE030_09650"
     CDS             complement(2147..2869)
                     /locus_tag="VE030_09650"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZF23716.1"
                     /translation="MSSIRRSRPAAPGRDALVSLTPGRPHRAGPDETKVFERCSRSMR
                     PERLLTYAPHYPNFGGQVSRPGAAGFDGRCTGTARSLGTAHLGRPAHRNAGGAMRKVM
                     VPVDGSECSLRAVQYLVDQRADRSNPADLEIHLVNVQVPLSGHIGQFIKREQVAAFQR
                     DEGAKMLQGARALLDASGVQYTAHTEVGSTAEVIVRLADSLQCDHIVMGTHGRGAFAE
                     LLVGSTTLKVVHLARVPVVLIK"
     gene            complement(2989..3495)
                     /locus_tag="VE030_09655"
     CDS             complement(2989..3495)
                     /locus_tag="VE030_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZF23717.1"
                     /translation="MNTRTVRVLACALSALGLCLAAVAAMAGGDIQPARIPATDIPWP
                     DSSARVEGTGMRTGLQSLVVAGDAKSNGLFTMMFRIPANTRIPPYSHPDVRSCFVLSG
                     TWFFGYGERADESQLKLLPAGSHYTEPAGMNHFAETRDEPVIAECTAIGPTGTTFVNP
                     DDDPRRKP"
     gene            complement(3650..4279)
                     /gene="msrA"
                     /locus_tag="VE030_09660"
     CDS             complement(3650..4279)
                     /gene="msrA"
                     /locus_tag="VE030_09660"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HZF23718.1"
                     /translation="MDQKIRNAILLVGGFVLLFAIDGMAAQEATPTPSGKNATAIFAG
                     GCFWCVEADFDKLPGVLGTESGYTGGAVSNPTYEQVSHGGTGHAEAVRVTYDPAKVTY
                     EQLLDYFWRHVDPTVKDRQFCDIGNQYRTAIFYQGDAQRLAAEASKTRLEKSGHFPRI
                     YTEVVPASAFYAAEEYHQDYYKKNPIRYKYYRTSCRRDARVAEVWGEKH"
BASE COUNT          845 a         1418 c         1348 g          782 t
ORIGIN      
        1 cagcagcccg gcgaatggtc cctggggcgg gcgggcgaac gaaaccggct gcccgcgctt
       61 gcgccagccg cgtaccgtgc gcgcgaacaa ggggcggaac aggcgcatga accaccggtc
      121 ggcgagttgc tcccaggaat ggggtggcag cgccgggatg gtggaaatgc gattgaagaa
      181 tgcgatgcag gcggcggtga aggcgatctc gcgtatctcc gcgtcgctgt atccggcggc
      241 gcgcagcggc tcgaggtcct gcggcgatac cagcggggtg gcgcgcgtca tgcggcgccc
      301 gaagttcacg gcggcggcgg ccttcggttc cagctcaccg ctcgcaaggc gttgctcgag
      361 ttgttgcatg cgctcttcgc tcatgccgag gacgcgcaat gccatgcgcg tgatcgcgta
      421 gcagtagcgg caggagtttt cctggctcac caccagcgca atcaggtcca cggtggggaa
      481 atcaacgtgc accagcagcc cgttgtctat gcccagtcgg attgccgccc tgggcaacca
      541 gggacaactc agaaaatagc gtatccagcc cggcgcggcg ccggtttccc ggcgcaacgc
      601 caactcggcg gcccggtcgg gcgccggatc gagcaggcag tggtcccagg acacattgag
      661 cagcgggctc ggcatcgcgt tactttaatc gaaaatctag cgaatgtatg ggagcaccca
      721 gatcagcatg ccgaacaagt aacatacgcc cgccgcgacc agcgtgaacg gcacgctcca
      781 ggacaggaac tcgcgcagcg agaccttgcg cccttcctcg cgctcgcaaa tattgagtgc
      841 ggcgaatagt gcgggcgcgc cggccacggt caggttgctt cccagggtcc cggaccacag
      901 cagcatccac cacagcggcc agggtggcac accctcgctc tgcaggtccc ggatcgtgta
      961 gagaaacgtc aggatatacg cgtcgtgctc gacgattccg acgattggaa ccgtggtcca
     1021 atagagcagc gtggcgccca gcgcgaagtt ctcagagaat accggccgta tctggtgcgc
     1081 aagcgcgtcg atcagattca tcttctccaa cccgccgacc agcgcgaaca acgcgacgta
     1141 gaagaagatc gctcgccaat cgaactcgcc gaggatcttc tcgagggccg gcgccttcac
     1201 gcgctcgccc aacagctcga gcgccagtac cagcgtgatg gcgccggtca gcgcgatgaa
     1261 gccgagcttc acgcccagca tctcccggaa cgccattgcc agcaccgtca gcaggaacca
     1321 cccgaccacc acgcaggcga acaggcggtt cggaatatgc gtctcgatgc cgaggctccc
     1381 gatatcgacc gcacgccggc tgtgggtccg gaagccgtag gcgtacatcg ccgcgagcgt
     1441 tacgatgaaa gtcgccatca ggatcggaaa ggaagaaggc cgcccctggc tccagatgaa
     1501 ctccatgaac tcggctccgg agatgctgtg cagcatctgc ggcggaaggt cccccagcag
     1561 cagggcggag cccatgaagt ttgccgagaa gccgatcatc aataccagcg gcgtgatcgg
     1621 caatctgagc gcgcgcgcca ggggcaaagc caccggcgcc atcatcagga taaccaccac
     1681 gttgtccacg aacatcgaga tcacgcccgc cagcatcgac agaatcatca ccaacagacc
     1741 agggcgtccg cccgacagcc gcagcgcctg taccgacagc cagctcggca cgccggtctt
     1801 gccgaagtaa ccggcgatca tccagatgct gatgaggatg gccatcacgt tccagtcgac
     1861 gagttgaaac gcttcgacct cggtcatcac gccgagccag accattaccg cgacacctat
     1921 cagggctgcg atcgtgccgt cgacaacgtt gcttatgacg gcaaggatag acaacacgaa
     1981 gatcgccagc gccacccacg ccatcatctc catcgactgt cccctccgct gctcgagcgt
     2041 tgcatgcatg cccccagacg gcatcacgac cggtccatca atcaagcccg cctcgtgagg
     2101 gaaggcagag gcgccctcca gcgaagggtg ccggcgggtc gtgcaattac ttgatcagga
     2161 ctacgggcac gcgggcaagg tgcaccacct tcagcgtggt cgagccgacc agcagttcag
     2221 caaatgcgcc gcgtccgtgg gtgcccatca cgatgtggtc gcactggagc gagtcggcga
     2281 gccttacgat cacctcggcg gtcgatccga cttcggtatg tgcggtgtac tgcacaccgc
     2341 tcgcgtcgag cagtgcacgg gcgccttgca gcatcttggc gccttcgtca cgctgaaagg
     2401 cggcgacctg ctcgcgcttg ataaattggc cgatatggcc cgaaagcggg acctgtacgt
     2461 tgaccaggtg gatttcgaga tcggcagggt tcgaccgatc ggcacgctgg tcgacaagat
     2521 actggacagc gcgcaatgag cactccgatc cgtcgaccgg caccatcact ttcctcatgg
     2581 ccccccctgc attgcgatgc gcaggccggc cgaggtgggc agtcccgagc gaccgcgctg
     2641 ttccagtgca acgcccgtca aagccggcgg ccccgggccg agacacttgg ccgccgaagt
     2701 tagggtaatg cggggcgtac gtcaacagtc gctccggccg cattgaccgc gaacaacgtt
     2761 cgaatacttt agtctcgtcc ggccctgccc ggtgaggacg accgggtgtt aagctcacca
     2821 gagcgtcgcg gcccggagcg gcgggtcgag accggcggat cgaactcatt tattttctgg
     2881 cgggggaggt atcgaccatg aagacatttg cggcattcat ggttggcgtc atgttgactg
     2941 ttgcggtgtt cctgtccggt ctgaagaggg gcgtggcgca agacccggtc acggtttccg
     3001 gcgcgggtca tcgtccgggt tgacgaaggt cgtgccggtg ggtccgatcg cagtgcactc
     3061 ggcgatcacc ggctcgtcgc gtgtctccgc gaaatggttc attcccgccg gctcggtata
     3121 gtggctgccg gccggcaaca gcttcagctg cgactcgtcg gcccgctcgc cgtaaccgaa
     3181 gaaccaggtc ccggagagta cgaagcacga gcgcacgtcc gggtgcgagt acggcgggat
     3241 cctggtgttg gccgggatcc tgaacatcat ggtgaacagg ccgttgcttt tcgcgtctcc
     3301 ggccacgacc aggctctgca ggcccgtgcg catccccgtg ccttcgacgc gcgcggagct
     3361 gtcgggccag ggaatgtcgg tcgccggaat gcgtgccggc tggatgtcgc cgcccgccat
     3421 cgcagcaaca gccgccaggc acaggccaag cgctgaaagc gcacatgcaa gaacacgaac
     3481 ggtgcgggta ttcatctgta tccccctgtt cccggctgcc tggtcttcga gatacgccat
     3541 cagttctcga tcgtgattcg cgtcgggatc cgcccctcgt cactggagtt cttccctagc
     3601 ccctaatccc cagtccctgc agtttctccc tcgcccctca tccatgcctt taatgctttt
     3661 ccccccagac ctcggcaacg cgtgcatcgc ggcgacagct ggtccgatag tacttgtagc
     3721 gaatcggatt tttcttgtag tagtcctgat ggtattcctc ggcagcataa aaggcgctgg
     3781 ccggcacgac ctcggtgtag atcctgggaa aatggccgct cttttccagc cgagtcttgc
     3841 tggcttcggc cgcaagcctc tgcgcgtcgc cctggtaaaa aatggccgtg cggtactggt
     3901 tgccgatgtc gcagaactgc cggtccttca cggtcggatc gacgtgacgc cagaaataat
     3961 ccaggagctg ttcgtatgtg accttcgccg gatcataggt tacgcgcacc gcctcggcat
     4021 gaccggtgcc gccgtggctg acctgttcgt aggtcgggtt gctgaccgcg ccgccggtat
     4081 aacccgactc ggtacccaac acgccgggaa gcttgtcgaa atccgcctcc acgcaccaga
     4141 agcagccgcc ggcaaagatg gccgtggcgt ttttccccga cggcgtcggc gtggcctctt
     4201 gtgccgccat gccgtcaatg gcgaacagaa gaacaaatcc cccgacgagc aaaatcgcgt
     4261 tgcgaatctt ctggtccatg ttgtctgttc ccggttgtct ggtcttcgag ggacgccacc
     4321 aaatcactat cgccgtaaga ccagcaataa gttcatggtc tgatctatcc cttcaccctt
     4381 cacccttgct ctt
//
LOCUS       DASQOP010000241         7501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_922977, whole genome shotgun sequence.
ACCESSION   DASQOP010000241 DASQOP010000000
VERSION     DASQOP010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7501
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922977"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..391
                     /locus_tag="VE030_09665"
     CDS             104..391
                     /locus_tag="VE030_09665"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZF23719.1"
                     /translation="MISKQGERYLIEGPVTLAGVTALLAEGSRSFEGTPVVVDFSGVT
                     EADSSAISLMLEWTRRMHGGNRQIFFANLGESLVSIANLYGVKDLIPIAAE"
     gene            428..1330
                     /locus_tag="VE030_09670"
     CDS             428..1330
                     /locus_tag="VE030_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZF23720.1"
                     /translation="MVPAIEIRGVSKTYGSVRALCDIDLEIPQGEFFALLGPNGAGKT
                     TLISMLAGLARANAGSLKVMGHDVVADYRQARRALGVVPQELVFDPFFTVRESLRFQS
                     GYFGLRRNGDWIDEVMARLDLTDRADFNMRALSGGMKRRVLVAQALVHKPPVIILDEP
                     TAGVDVELRQGLWQFVRQLNGDGHTIVLTTHYLEEAESLCSRVGMLKQGKLVALDSTH
                     NLLRGFSGIHLRLRLANGALPESLRPMLAAAQVSGEYVLALHDYASVEGVLRTIRDNG
                     LELAAMELEQPDLEEVFVAIMRRE"
     gene            1338..2093
                     /locus_tag="VE030_09675"
     CDS             1338..2093
                     /locus_tag="VE030_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZF23721.1"
                     /translation="MTGFSTLFYKEILRFWKVSFQTILAPVLTALLYLLIFSRVPEEH
                     LQVYPGVRYTSFLVPGLAMMSLLQNAFANSSSSLIQSKITGNIVFILLPPLSHLEFFG
                     AYVLASAVRGAVVGLGVFVITAVFVPVPVQSPLWVVAFALLGSAILGTLGIIAGIWAD
                     KFDQLAAFQNFVIIPLTFLSGVFYSIHSLPPFWQHVSQFNPFFYMIDGFRHGFFGVSD
                     VSPYLSLAIVGGSLLALSFATLSMLKRGYKLRH"
     gene            2107..2343
                     /locus_tag="VE030_09680"
     CDS             2107..2343
                     /locus_tag="VE030_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HZF23722.1"
                     /translation="MATPEQVRSYIQDSLACDHVEVVGDGHHFEAIIVSPEFRGKTRV
                     QQHQVVYRALGDRMREEIHALSMKTLTPEDWAAR"
     gene            2361..3614
                     /gene="murA"
                     /locus_tag="VE030_09685"
     CDS             2361..3614
                     /gene="murA"
                     /locus_tag="VE030_09685"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HZF23723.1"
                     /translation="MDKLIIQGGVPLTGEVRISGAKNAALPILCAAILTPETLRVQNL
                     PHLRDVTTTLNLLAQMGVEISLDEKMGVGLTAAKLHNLAAPYELVKTMRASVLVLGPM
                     VARFGEARVSLPGGCAIGQRPVDLHIKGLQAMGAQIEIEQGYLHARARRLKAARIVMD
                     LVTVTGTENLMMAATLADGITVIENAAREPEVVDLAECLKAMGARIFGAGSDVITIEG
                     VEHLAGATYRVMPDRIETGTFLVAAAASGGEIRLKGTRAGILDSVLVKLREAGAHIEH
                     DQDWIALSTNSKLRSVSLRTAPYPAFPTDMQAQFMALNAIAEGTAVVTETIFENRFMH
                     VQELRRLGAKIDVEGNTAVVHGVAQLQGAGVMATDLRASASLVLAGLVAQGETRVDRV
                     YHLDRGYECIEEKLSQLGARIKRTN"
     gene            3623..4273
                     /gene="hisG"
                     /locus_tag="VE030_09690"
     CDS             3623..4273
                     /gene="hisG"
                     /locus_tag="VE030_09690"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HZF23724.1"
                     /translation="MNIKIALSKGRIFDETTPLLKAAGIVVSEDPETSRKLIIGTSRP
                     EIGIIIVRASDVPTYVQYGAADLGIAGKDVLLEHAGTGLYQPLDLGIAKCKMMVAVQN
                     GFDYEGAVRRGARLRVATKYLKTAREHFAAKGVHVDLIKLYGSMELAPLVGLADAIVD
                     LVSSGNTLRANGLQAVEEIMPISARLIVNQAALKLKRETIQPLLDAFAGAVAKSGT"
     gene            4270..5571
                     /gene="hisD"
                     /locus_tag="VE030_09695"
     CDS             4270..5571
                     /gene="hisD"
                     /locus_tag="VE030_09695"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HZF23725.1"
                     /translation="MIGIARLSTRDAGFDARLAGLLAFENTQDQRIEDAAAAILREVR
                     ARGDEAVLEYTRKFDHLEARSLAQLEIGKADLARALDVLPGTRRNALEQAAARIRAFH
                     ERQPLASWQYTEADGTTLGQKITPLDRVGVYVPGGKAAYPSTVLMNTLPAKVAGVKEI
                     IMVVPTPRGERNDLVLAAACIAGVDRVFTIGGAQAVAALAYGTRTVPQVDKIVGPGNA
                     YVAAAKRRVFGVVGIDMVAGPSEILVICDGGTDPDWVAMDLFSQAEHDEMAQAILLCP
                     ERAYIDKVAASIERQIKDMPRAGVITAALEGRGALIETRDLNEACEIANRIAPEHLEL
                     SVDEPQRWVEKIRHAGAVFMGRYTSEALGDYCAGPNHVLPTARSARFSSPLGVYDFQK
                     RTSLIQVSADGARKLGAIAAELATGEGLPAHARAAQLRLRR"
     gene            5568..6662
                     /gene="hisC"
                     /locus_tag="VE030_09700"
     CDS             5568..6662
                     /gene="hisC"
                     /locus_tag="VE030_09700"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HZF23726.1"
                     /translation="MSRYWSAVVHGLTPYVPGEQPRLPNLVKLNTNENPYGPSPRVLE
                     ALRGEVADSLRLYPDPTSQRLVQAVAARHGVVSSQVFVGNGSDEVLAHTFQALLRHDQ
                     PVLFPDVTYSFYPVYCRLYGIAYREIPLTDSLEIDVDDYLQTSNGGIVFPNPNAPTGR
                     LLGLSDIERLLQGNTASVVVIDEAYVDFGGTSAVSLVHRYPQLLVTHTLSKSRSLAGL
                     RVGYAIGHPELIEALIRVKDSFNSYPMDRFAQAGAIAALEDQNYFEAACRKVIRSRER
                     LVAGLQSLGFEVLPSAANFVFARHPRWEGAALTARLREQGIVVRHFRVPARIATFMRI
                     TVGTDEHCDTLLDALRGIFLDAGIASSATP"
     gene            6699..7286
                     /gene="hisB"
                     /locus_tag="VE030_09705"
     CDS             6699..7286
                     /gene="hisB"
                     /locus_tag="VE030_09705"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HZF23727.1"
                     /translation="MRQAQVTRNTLETQISVKLNLDGSGKSKLATGIGFFDHMLDQVA
                     RHGMIDLEIEAKGDLHIDAHHTVEDVGITLGQAFAKAIGDKKGVRRYGHAYVPLDEAL
                     SRVVVDLSGRPGLEYAVDYARGLVGEFDIDLVHEFFQGFANHALVTLHIDNFKGRNAH
                     HQAETIFKAFGRALRMASELDPRAAGALPSTKGSL"
BASE COUNT         1367 a         2267 c         2446 g         1421 t
ORIGIN      
        1 acctgttcgc tctggagatc gatcgtggcg gaatcgacgg cctgatcaag agtctcgcca
       61 acaggaatag cgccaatgcc aatacggcgc ccgccggcaa acaatgatca gcaaacaggg
      121 cgagcgttac ctgatcgaag gtccggtgac gctggcgggt gtcaccgcgt tgctcgccga
      181 gggcagccgc agtttcgaag gcactcccgt ggtcgtggat ttctccgggg tgacggaagc
      241 cgactcttcg gcaatcagcc tgatgctgga gtggacccgg cgcatgcacg gcggcaaccg
      301 ccagatcttc ttcgccaacc tcggcgaaag cctggtgagc attgccaatc tctacggggt
      361 caaagacctg attcccattg cggcagaata gccgccgatc cagaagaaaa cactgcacaa
      421 cgtcgctgtg gtcccagcca tcgagatacg cggcgtctcc aaaacctacg gaagcgtacg
      481 cgcgctttgt gacatcgacc tggaaattcc tcagggtgag ttcttcgcgc tgctcggacc
      541 caatggcgct ggcaagacca cgctgatcag catgctggca ggactggcgc gcgccaacgc
      601 gggttcgctc aaggtgatgg gccacgacgt ggtggccgat taccggcagg cgcggcgtgc
      661 gctcggcgtg gtgccgcagg agctggtgtt cgacccgttc ttcacggtac gggagagttt
      721 gcgtttccag tcagggtatt tcggcttgcg tcgaaatgga gattggatcg atgaagtgat
      781 ggcgcgcctg gacctcaccg accgcgccga cttcaatatg cgcgcgctat ccggcgggat
      841 gaaacggcgg gtgctggtgg cgcaggccct ggtacacaag ccgccggtga ttattctcga
      901 cgagccgacc gccggcgtgg atgtcgagtt gcggcagggc ctgtggcaat tcgtccgcca
      961 gctcaacggt gacggccaca ccatcgtgct gaccacgcac tatctggaag aggccgagtc
     1021 gttgtgcagc cgtgtgggca tgctcaagca ggggaagctg gtcgcgctgg attccacgca
     1081 taacctcctc aggggattct cgggtattca cctgcggctg cggcttgcca acggtgcgct
     1141 gcccgagagt ttgcgcccga tgctggcggc cgcccaggtg agcggcgagt atgtgctggc
     1201 gctgcatgat tacgccagtg tcgaaggcgt gttgcggacc atccgcgaca acgggctcga
     1261 gctggcggcc atggaactgg aacagcccga cctggaagaa gtcttcgtcg ccatcatgcg
     1321 cagggagtga aacgacgatg accggtttct ccacgctgtt ctacaaggag atcctgcgtt
     1381 tctggaaggt gagtttccag accatcctgg cgccggtgct caccgcgctc ctgtatctgc
     1441 tgatcttctc gcgcgtgccg gaagagcatt tgcaggtcta cccgggcgtg cgctatacat
     1501 cgtttctggt acccggcctg gcgatgatgt cgctgctgca gaatgccttt gccaacagct
     1561 cgtcgagcct gatccagtcc aagatcaccg gaaatatcgt cttcatcctg ctgccgccgt
     1621 tgtcgcacct cgaattcttc ggcgcctatg tgctggcttc cgccgtgcgg ggtgcagtgg
     1681 tcggactcgg cgtgttcgtg attaccgcgg tgtttgtccc ggtgccggtc cagagtccgc
     1741 tgtgggtggt tgcctttgcg ttgctcggat cggcgatcct gggtacgctc ggcatcatcg
     1801 ccggcatctg ggccgacaag ttcgatcagc ttgccgcgtt ccagaatttc gtgatcatcc
     1861 cgcttacttt cctgtcgggc gtgttttact cgattcactc gctgccgccg ttctggcagc
     1921 acgtgtcgca gttcaatcct ttcttctaca tgatcgacgg ctttcgccac ggcttcttcg
     1981 gcgtatccga cgtctcgccg tatctcagtc tcgcgattgt ggggggctcc cttttggcct
     2041 tatcattcgc caccttgtcg atgctcaagc gcggttacaa gcttcgccac tgaggaagtc
     2101 gttgaaatgg ccacccccga acaggtcagg tcttacatcc aggacagcct cgcttgcgat
     2161 cacgtcgagg tggtcggcga cggccatcat ttcgaggcga tcatcgtaag cccggaattc
     2221 cgcggcaaga cgcgggtgca gcaacaccag gtggtctacc gggcgctggg ggatcgcatg
     2281 cgcgaggaaa tacacgcgtt gtcgatgaag accctgacgc ccgaggattg ggctgcccga
     2341 tagcctttca ataggtttcc gtggacaagc tgatcatcca gggcggcgtg ccgctaaccg
     2401 gggaagtgcg catttccggg gccaagaacg cggccctgcc aatcctgtgc gccgccatcc
     2461 tgacgccgga gacgctgcgg gtgcagaatc ttcctcacct gcgcgacgtg accacgacgc
     2521 tgaatctgct ggcgcaaatg ggcgtcgaga tttcgctcga tgagaagatg ggtgtgggcc
     2581 tgacggcggc gaagctgcac aacctggcgg cgccctacga gctggtcaag accatgcgcg
     2641 cctcggtcct ggtgctggga ccgatggtcg cgcgtttcgg cgaggcccgc gtgtcgctgc
     2701 cgggcggatg tgccatcggc caaagaccgg tggatctgca catcaagggt ttgcaggcga
     2761 tgggtgcaca gatcgagatc gaacagggct atcttcatgc ccgtgcccgc cggctgaaag
     2821 ccgcgcgcat cgtcatggat ctggtgactg tgaccggcac cgagaacctg atgatggcgg
     2881 cgaccctggc cgacggcatc accgtgatcg agaacgcggc ccgcgaaccg gaggtggtgg
     2941 atctggccga gtgcctgaag gcgatgggcg cgcgtatttt cggcgccggt tccgacgtga
     3001 tcacgatcga gggtgtggag cacctcgcgg gtgcgaccta ccgggttatg cccgatcgca
     3061 tcgaaacggg cacgttcctg gtggccgcgg cggccagcgg cggagaaatc aggttgaagg
     3121 gtacgcgcgc gggaattctg gactcggtgc tggtcaagct gcgcgaggcc ggtgcacaca
     3181 tcgagcatga ccaggactgg attgcccttt ccaccaacag caagctcagg tcggtgagcc
     3241 tgcgcacggc gccgtatccg gccttcccga ccgacatgca ggcccagttc atggcgctca
     3301 acgccattgc cgaagggacg gccgtggtga ccgaaaccat tttcgagaac cgcttcatgc
     3361 atgtacagga actgcggcga ctcggggcaa agatcgatgt ggaaggaaac acggcggtgg
     3421 tgcacggcgt ggcgcaattg cagggcgccg gcgtgatggc caccgatttg cgcgcctcag
     3481 cgagcctggt gctggccggg ctggtggcgc agggcgaaac gcgggtggac cgtgtgtacc
     3541 acctcgatcg cggctacgaa tgtatcgaag agaaattgag ccagttgggc gcccgcatca
     3601 aacgtacgaa ttagcctgaa agatgaacat caagatcgcc ctgtcgaagg ggcgcatttt
     3661 cgacgaaacg acgccgctcc tgaaagcagc gggaatcgtc gtatctgagg atccggaaac
     3721 ctcgcgcaag ctgatcatcg gcaccagccg gccggagatc ggcatcatca tcgtgcgcgc
     3781 ttccgacgtg ccgacctacg tccagtatgg cgcagccgat ttggggatcg ccggcaagga
     3841 tgtgctgctc gagcacgcgg gaaccgggct gtaccagccg ctcgacctgg gaatcgcaaa
     3901 gtgcaaaatg atggtggcgg tgcagaacgg cttcgactat gagggcgcgg tgcgccgcgg
     3961 cgcgcgcctg cgggtggcta ccaagtacct gaagacggcg cgcgagcatt tcgctgccaa
     4021 gggggtgcat gttgacctga tcaagcttta tggctcgatg gaactggcgc cgctggtggg
     4081 cctggcagac gcgatcgtgg acctggtttc cagcggcaat acgctgcgcg ccaatggtct
     4141 ccaggcggtc gaggagatca tgccgatcag tgctcgcctg atcgtaaacc aggccgcact
     4201 caagctcaag cgcgagacga tccagccgct gctggatgct ttcgccgggg ccgtggcaaa
     4261 gagcggaaca tgatcgggat tgcacgcctg tctacccgcg acgccggctt cgatgcgagg
     4321 ctggccggct tgctcgcatt cgagaatacc caggaccagc gcatcgaaga cgcggcggcg
     4381 gcgattctgc gcgaggtcag ggcgcgcggc gacgaggcgg tgctcgagta cacgcgcaag
     4441 ttcgatcatc tagaggcgcg ctcgcttgcg caactcgaaa ttggcaaggc cgatctggcg
     4501 cgcgcgctcg acgtgctgcc gggcacgcgg cgcaatgcgc tggagcaggc cgcggcgcgc
     4561 atccgtgcct tccacgaacg ccagccgctg gcgtcctggc aatacaccga ggccgacggt
     4621 acgacgctcg gtcaaaagat cacgccgctc gatcgcgtcg gagtgtatgt accgggcggc
     4681 aaggctgctt acccgtccac cgtgctgatg aacacgctgc cggccaaggt ggcgggcgtg
     4741 aaggaaatca tcatggtggt gcccacgccg cgcggtgagc gcaacgatct cgtgctcgcc
     4801 gccgcgtgca tcgccggagt ggatcgcgtg tttaccatcg gcggcgccca agccgtggcg
     4861 gcgctggcct acggcacacg gaccgttccc caggtggaca agatcgtcgg cccgggcaac
     4921 gcctacgtcg cagccgcgaa acggcgcgtc ttcggcgtcg ttggaatcga tatggtggcg
     4981 ggcccatccg agattctggt gatttgcgac ggcggcaccg atccggactg ggtggcgatg
     5041 gacctgttct cgcaggccga gcacgacgag atggcgcagg cgatcctgct ctgcccggag
     5101 cgcgcataca tcgacaaggt agcggcgagc atcgagcggc agatcaaaga tatgccgcgt
     5161 gcaggcgtga tcaccgctgc gctcgaggga cgcggtgcgc tgatcgaaac ccgcgatctc
     5221 aacgaggcct gcgagatcgc caatcggatc gcccccgagc acctggaatt gtcggtggac
     5281 gagccgcagc gctgggtcga gaaaatcagg catgctggtg ccgtgttcat gggccgttac
     5341 acctccgagg cgctggggga ctactgcgcg ggaccaaatc acgtgctgcc gaccgcgcgc
     5401 agcgcgcgat tctcctcccc gctcggggta tacgactttc agaaacgcac cagcctgatc
     5461 caggtttccg cggacggcgc acgcaagctc ggcgcgattg ccgccgagct cgcgaccggc
     5521 gagggcctgc cggcacatgc gcgtgccgcc cagttgcgat tgcgcagatg agccgttact
     5581 ggagcgccgt ggtccacggc ctgacgcctt acgtgccggg agagcaaccc aggctgccca
     5641 acctggtgaa gctcaacacc aacgaaaatc cgtacggtcc cagcccacga gtgctggagg
     5701 cactgcgggg cgaggtggcg gattcgctgc gcctataccc cgatcccaca agccagagac
     5761 tggttcaggc cgtggccgcg cggcacgggg ttgtttccag ccaggtcttt gtcggcaatg
     5821 gctccgacga agtcctcgcg cacacgttcc aggcgctgct cagacacgac cagccggtgt
     5881 tgtttccgga tgtcacttac agcttctacc cggtgtactg ccgtttgtac ggcatcgctt
     5941 atcgcgagat tccgctgacg gattcgctcg agatcgacgt cgatgactat ttgcagacga
     6001 gcaatggcgg gatagtgttt cccaatccaa atgcaccgac cgggcgcctg ctgggcttat
     6061 ccgatatcga gcggctcctg cagggcaaca cggcatccgt agtcgtcatc gacgaggctt
     6121 acgtggattt cggcgggaca tccgccgtgt cgctggtgca ccgctacccc cagttgctgg
     6181 tgacgcacac cctgtccaaa tcccgctcgc tggcggggct gcgcgtgggc tatgcgatcg
     6241 ggcatccgga attgatcgag gcgctgatcc gggtcaagga cagtttcaac tcttatccga
     6301 tggaccgctt cgcccaggcc ggagccatag cggcgttgga ggaccagaat tatttcgaag
     6361 cggcctgtcg caaggtgatc cgtagccgcg agcggctggt tgccgggctt cagtcgctgg
     6421 gcttcgaggt cctgccctcc gcggcaaact tcgtcttcgc ccgccatccg cgctgggaag
     6481 gcgcggcgct gacggccagg ctacgcgagc aaggcattgt cgtgcggcat ttccgcgttc
     6541 cggcccgcat agccacgttc atgcgcatta cggtgggaac ggatgagcac tgcgatacgc
     6601 tgctcgatgc cttgcgcggg atttttctcg acgcgggtat cgcctcgtcg gccacgccct
     6661 aattgagtta ggatgaagca atttttgacg cctatcgtat gcgccaagcc caagtcaccc
     6721 gcaataccct ggaaacccag atcagcgtca agcttaatct cgatggcagc ggcaagtcca
     6781 agcttgccac cggcatcgga ttcttcgatc acatgctcga tcaggtggcg cgccacggca
     6841 tgatcgacct ggaaatcgaa gccaagggcg acctgcatat cgacgcccac cacaccgtcg
     6901 aggatgtggg catcacgctc gggcaggcct tcgccaaggc gatcggcgac aagaaaggtg
     6961 tacgccgcta tggccatgcc tacgtccctc tcgacgaagc gctgtcgcgg gtggtggtgg
     7021 atctgtcggg ccgccccggg cttgagtatg cggtcgatta cgcgcgcggg ctggtcggcg
     7081 aattcgacat cgacctggtc catgaattct tccagggctt cgctaaccac gcgctggtga
     7141 ctttgcacat cgacaacttc aaaggcagga acgcgcacca ccaggcggag actatcttca
     7201 aggccttcgg gcgcgcgttg cgcatggctt ccgagttgga tccgcgggcg gccggcgcgt
     7261 tgccttcgac caaggggagc ctgtgagcgt tttcgcggat cgggtttccg gccgcatatg
     7321 cgtcggcaag taatgttgaa gacgacccaa gagtcacgaa ccgcgcgtgt atgaccgata
     7381 ttgctgtcgt tgattacgga atgggcaacc tgcgttcagt ggctaaggcc ttggagcatg
     7441 tcgccccccg caaggttgtt agcgtgacca gtgatccggt agtgctacgc tcagcatcca
     7501 g
//
LOCUS       DASQOP010000242         7955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2332267, whole genome shotgun sequence.
ACCESSION   DASQOP010000242 DASQOP010000000
VERSION     DASQOP010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7955
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2332267"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VE030_09710"
     CDS             complement(<1..187)
                     /locus_tag="VE030_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23728.1"
                     /translation="MNKTVWAFGLAWLLTLSTPTQSQQPQAPADYPSWAFPLKVEKRL
                     PSAGPEPMSLPGSPARYT"
     gene            complement(248..904)
                     /locus_tag="VE030_09715"
     CDS             complement(248..904)
                     /locus_tag="VE030_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZF23729.1"
                     /translation="MLRIEALDVSIQSVGVLRGVNMEVPTGKFAGLIGRNGAGKTTLM
                     RSVMGILPARQGTIRFDEVSLDRMPAHRRARGGIGYMPEDRRLVPQLTVEENVLIPAW
                     AAGVAEPQSRLSQVYRMIPEVEAFAGRKALQLSGGQQKLVALARALMCGTRLLLLDEP
                     FEGVAPVLAHRLAEVIAGLKSQGLSVILTESDLSHSQEILDVVYSIDRGEVSIATSPA
                     "
     gene            complement(891..1673)
                     /locus_tag="VE030_09720"
     CDS             complement(891..1673)
                     /locus_tag="VE030_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZF23730.1"
                     /translation="MAVVLEARGLNKNFGAVTAAADINVAVERDSVVGLIGSNGAGKT
                     TFINMVTGYLKPSSGSIAFGSRDITPLAPRQITRLGICRSFQIPQLFNTLSVYENLLV
                     GVGIVASGGAKWADASRHAGIPSETVDSILERFNLTGYRYKPAGVLPEGVRKLLDIAM
                     AMAVKPKILLLDEPTSGVSADEKFGLMDMVMEAVRAQQVTVLFVEHDMDIVNRYTHRV
                     LAFYEGRIIADGEPGVVLDDAEVRKYVTGEARAAGGGKHAAH"
     gene            complement(1675..2616)
                     /locus_tag="VE030_09725"
     CDS             complement(1675..2616)
                     /locus_tag="VE030_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZF23731.1"
                     /translation="MKRDFTIRTLVGVLIALLLAGPLLPRWAMFLITVSLCYGTVVLG
                     MMLLMRTGVVSFGHGLYYCLGAYASGTLDVLFGVSDIAVMLLAAVAVTGVVSALLGLL
                     LRKYRDIFFAMLSLAFSMILYGLLLKTSALGSSDGFNVAAKTLFGIRIAGTEFERYLI
                     FAVTVLIAFVAAVCMHKYLASHMGRLAGAIRDNELRVEYMGASVRNTIHVNYVMSAVL
                     AGLGGALAAVSVGHIDPEMSYWTTSGEFVFVAILAGTGNVLAPFLGAFIFETIRSFAY
                     EYSPNTWQMALGTTMLLVIMFLPGGLWSLFTRMRRAT"
     gene            complement(2674..3570)
                     /locus_tag="VE030_09730"
     CDS             complement(2674..3570)
                     /locus_tag="VE030_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZF23732.1"
                     /translation="MNQLLAVLIDGLIYSSWLFIIAVGLTLIYGVMKILNIAHGSLYA
                     LGAYSAASLAGYWINQGHAPMGSYAMMLVAAILVGLIAGPVIERGLLRFMYGKDEIVL
                     VLVTYAVFLILEDFIKLTWGVDPYFLYQPYSLLGSFDVGNLTYPVYNLVLFGMAVLIG
                     VTLVWVLTKTRRGKMLVAVIHDREMAAAMGINVSLVYFVTFTAGAMLGAVGGALTAPM
                     ISVQPGIGAETIVLAFAVVVIGGLGSLPGAALAAVLVGLVRSAAVHYRPELDLFAIYF
                     VMAVVLIMRPKGLFSVQEARKI"
     gene            complement(3587..4888)
                     /locus_tag="VE030_09735"
     CDS             complement(3587..4888)
                     /locus_tag="VE030_09735"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZF23733.1"
                     /translation="MDWNFSRPLKTAAAVLAGATLAITCATAHAQDKPIRIGLVTFLS
                     GPAAGPFGVPAKMAADAIVDSLNAGKAPAPYNIKGFGGTPLELTIVDEAGGATKQVTE
                     LRNLIERQNVDFVIGYISSGDCLAVAPVAEELKTLTILFDCGTPRVFEEASYKYVFRT
                     RSHATMDAVAGAMYLLENRPAVKSYAGINQNYAWGQDSWADFTAVMATLKPEATITSS
                     QMPKFGAGQYGPEISALLQTNSDVIHSSLWGGDLEAFMLQATPRGLFNRSQLVLVAGE
                     PYLHRLAGNMPDGTVVGARGPNGVYAQKSALNDWLRQIYKERAEIYPNYPAYSVAMAF
                     LGLKAAYEKARGAGGGAPATDDVIKAFEFLNFDSPAGKVMMKLGKGHQAVTGTAYGTT
                     RTVNGQVTVGDIKYYALERVQPPEGVKSLDWIKSGMKPGNW"
     gene            complement(5034..5933)
                     /locus_tag="VE030_09740"
     CDS             complement(5034..5933)
                     /locus_tag="VE030_09740"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HZF23734.1"
                     /translation="MPAPDAATWRAIAADLSRVTGSPFPGSRAEPAGGGSINRAFRLD
                     GGERRFFVKLNSAWRLEMFEAELAGLMELAATKAVRVPAPICCGSDALHSWIVLEHLE
                     LGNGTDGLAALGAQLALMHRSTAANFGWSRDNTIGSTPQHNEWCSDWIVFLRERRIGF
                     QLQLAQRNAYRGRLQSSGEKLLAELDAFFAGYRPEASLLHGDLWGGNAAVLAGGEPAI
                     FDPAVYFGDREADLAMTELFGGFPQAFYRGYGDAWPLDPGYRVRKQLYNLYHLLNHLN
                     LFGSSYLAPAQAAIDGLLGEIRG"
     gene            complement(5957..7366)
                     /gene="argH"
                     /locus_tag="VE030_09745"
     CDS             complement(5957..7366)
                     /gene="argH"
                     /locus_tag="VE030_09745"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HZF23735.1"
                     /translation="MQPKEKPVDDAGKTRSGRFSEPVADLVKRYTASVDFDKRLAEFD
                     IAASLAHARMLHAVGVLTAKDLADIERGMSAILGEIRQGMFTWSVDLEDVHLNIEKRL
                     TDLVGNAGKRLHTGRSRNDQVATDIRLYLRWAVDEILALIHAFQKSLLDLAEHHVDTL
                     MPGYTHLQVAQPVSFAHHLLAYYEMMKRDAARFADCRRRFNVLPLGAAALAGTDYPID
                     REGVARELGFDGVCENSLDAVSDRDFAIEFCACAALTMTHLSRLSEELILWMNPRFGF
                     IALADRFCTGSSIMPQKKNPDVPELVRGKTGRVNGHLVALLTLMKGQPLAYNKDNQED
                     KEPLFDTVDTLTATLRIYADMVGGISVNKEAMRAALLEGYATATDLADYLVGKGLPFR
                     EAHEAVARAVRFAAEKGVDLTQLSVEQLRQFSKLIDTEAIGKLSLEGSVASRDHVGGT
                     APTQVRAAIARARAWLAAR"
     gene            7502..>7955
                     /locus_tag="VE030_09750"
     CDS             7502..>7955
                     /locus_tag="VE030_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZF23736.1"
                     /translation="MLRLLVMVIVMTVVAAVLKSSNWYDMLDQWLGISALAQPMVILS
                     LLLLGLADRWLHELDYPLAVAVIVAIELGIVSVVVIATSAVFSPDTPGLARSCFLTAV
                     ATLLVLAYFNLRSRMLSPALTEARLQALQARIRPHFLFNSINAVLSLVR"
BASE COUNT         1475 a         2529 c         2496 g         1455 t
ORIGIN      
        1 gtgtgtatct tgccgggcta ccgggcagcg acatgggctc cggccctgcc gacggaagcc
       61 gtttctccac cttcaagggg aatgcccacg acggatagtc ggctggcgcc tgcggctgtt
      121 gcgactgcgt tggcgttgac agcgttagca gccacgcgag accgaacgcc cataccgttt
      181 tgttcacgag aacctccctg caattggctg agaccaatga cgagctcgca gcccggattc
      241 gcgggctcta agccggcgat gtagcgatcg aaacctctcc gcggtcgatg ctgtaaacca
      301 cgtcgaggat ttcctgcgag tgcgagaggt cggactcggt gaggatgacc gagagcccct
      361 ggcttttcag cccggcaatc acttccgcga gccggtgcgc cagcaccggc gccacgccct
      421 cgaacggctc gtcgagcagc agcagcctgg tgccgcacat cagtgcgcgc gccagcgcca
      481 ccagtttctg ctggccgccg gaaagctgca acgccttgcg gccagcgaag gcttcgactt
      541 cggggatcat cctgtatacc tgcgacagtc gcgactgcgg ttccgcaacg cccgctgccc
      601 acgccggaat gagtacgttt tcctcgacgg tcagttgcgg aacgaggcgg cggtcctcgg
      661 gcatgtaacc gatgccaccg cgtgcacgcc ggtgggccgg catcctgtcg agcgacactt
      721 cgtcgaaacg gatggtgccc tggcgcgccg ggagaatgcc catcaccgag cgcatcagcg
      781 tggtcttgcc ggcgccattg cggccgatca ggccggcgaa ctttccggtc ggcacctcca
      841 tgttcacccc gcgcaggacg ccgaccgact ggatggagac atcgagcgct tcaatgcgca
      901 gcatgcttgc ctccgccggc ggcgcgcgcc tcgccggtca cgtacttgcg cacttcggca
      961 tcgtcgagaa cgacgccggg ttcgccgtcg gcgatgatcc ggccctcata gaacgccagc
     1021 acgcgatggg tatagcggtt gacgatatcc atgtcgtgct cgacgaacag caccgtgacc
     1081 tgttgcgcgc gtaccgcttc catgaccatg tccatcaacc cgaacttctc gtccgccgac
     1141 acgcccgagg tgggttcgtc gagcagcagg atcttcggct tcaccgccat ggccatggcg
     1201 atgtcgagca gcttgcgcac accctcgggc aacacgccgg ccggcttgta gcgatagccg
     1261 gtgagattga agcgctccag tatcgagtcc actgtctcgc tggggatgcc ggcatggcgg
     1321 ctcgcatccg cccacttcgc gccgccggag gcgacgatgc cgacgccgac cagcaggttc
     1381 tcatacaccg acagtgtgtt gaacaactgc gggatctgaa acgaacggca gatgccgagc
     1441 cgcgtgatct ggcgcggcgc cagcggcgtg atgtcgcggc ttccgaaggc gatgctgccg
     1501 gaactgggct tcaggtaacc ggtgaccatg ttgatgaacg tggtcttgcc cgccccgttg
     1561 ctaccgatca atcccaccac gctgtcccgt tcgacggcga cgttgatgtc cgccgctgcg
     1621 gtgaccgcgc cgaaattctt gttcagcccc ctggcttcca gaacgaccgc catgtcaggt
     1681 cgcccttcgc attctggtga acagcgacca caacccgccc ggcaggaaca tgatcacgag
     1741 cagcatggtg gtgccgagcg ccatctgcca ggtgttgggc gagtattcgt aggcgaagct
     1801 gcggatggtt tcgaaaatga acgcgccgag gaagggcgcc agcacgttgc cggtgccggc
     1861 cagaatggcc acgaagacga actccccgga agtggtccag taagacatct cgggatcgat
     1921 gtggccgacg ctcaccgcgg cgagcgcgcc acccagtccc gccagcaccg ccgacatcac
     1981 gtagttgaca tggattgtgt tgcgcaccga tgcgcccatg tactcgacgc gcaactcgtt
     2041 gtcgcggatc gccccggcca gccggcccat gtgcgatgcc aggtacttgt gcatgcaaac
     2101 ggcggcgacg aaggcgatga gcacggtcac ggcaaagatc aggtagcgct cgaactcggt
     2161 gcccgcgatc cgtattccga acagcgtctt cgccgcgacg ttaaaaccgt ccgaactgcc
     2221 cagcgcggaa gtcttcagca gcaggccgta gagaatcatc gaaaaggcaa gcgagagcat
     2281 ggcaaagaag atgtcgcggt acttgcgcag cagcaacccg agcagcgccg atactactcc
     2341 agtgaccgca accgccgcca gcaacatgac cgcgatatcc gagacaccga acaggacgtc
     2401 gagcgtaccg gaggcgtaag cgcccagaca gtagtacaag ccgtggccga aagacaccac
     2461 accggtgcgc atcagcagca tcatgccgag cacgaccgtg ccgtagcaca gcgatacagt
     2521 gatgaggaac atggcccagc gcggcaggag cggccccgcc aggagcagcg caatcagtac
     2581 gccgacaagt gtgcggatgg tgaagtcgcg cttcaaatct ttcgtgcctc ctccgccaga
     2641 gacatcatat tttgcgtgct tcctgcgcca gggtcatatt ttgcgtgctt cctgcacgct
     2701 gaacaggccc ttgggacgca tgatgagtac caccgccatg acgaaataga tcgcgaacaa
     2761 gtcgagctca ggccggtagt gaacagcggc cgaacgcact aggccgacca ggacggcggc
     2821 cagcgccgcg ccgggcagac tgccgaggcc gccgatcacc acgaccgcga aggcgagcac
     2881 gatagtctcg gcgccgatcc cgggttggac tgaaatcatg ggcgcggtga gtgcgccgcc
     2941 gacggcgccg agcatggcgc cggcggtgaa ggtgacgaag taaaccaggc ttacgttgat
     3001 gcccattgcc gcggccatct cgcggtcgtg aatgaccgcg accagcatct tgccgcgacg
     3061 ggtcttggtc agcacccaga ccagagtgac gccgatcaga acggccatgc cgaacagcac
     3121 caggttatag accggatacg tgaggttgcc cacgtcgaaa ctgccgagca ggctgtacgg
     3181 ctggtacaga aagtagggat cgacgcccca ggtgagcttg atgaaatcct ccaggatgag
     3241 gaacaccgcg taggtgacca gcaccaggac gatttcatcc ttgccgtaca tgaatcgcag
     3301 cagaccccgt tcgatcaccg gccccgcgat cagcccgacc aggatcgcgg cgaccagcat
     3361 catcgcgtaa cttcccatcg gggcgtgacc ctggttgatc cagtagccgg cgagagaagc
     3421 ggcgctgtaa gcgcccagcg cgtacaggct gccgtgggcg atgttcagga ttttcatcac
     3481 gccgtaaatc agcgtgagtc cgacggcgat gatgaacagc cacgacgagt agatcagccc
     3541 gtcgatgagg accgcgagca gctggttcat ttcggcatgc gcggccttac cagttgccgg
     3601 gcttcatccc ggacttgatc cagtccaggc ttttcacgcc ttcgggaggc tggacgcgct
     3661 ccagcgcgta gtacttgatg tcgccgaccg tcacctggcc gttgaccgtg cgggtggtgc
     3721 cgtaggcggt tccggtcacc gcctggtggc ccttgccgag cttcatcatg acctttccgg
     3781 cgggcgaatc gaaattgagg aattcgaatg ccttgatcac atcgtcggtg gcgggcgcgc
     3841 cgccgccggc cccacgcgct ttctcgtagg ccgccttcag tcccagaaac gccatcgcga
     3901 cgctgtaggc cggatagttc ggataaattt cggcgcgctc cttgtagatc tggcgcagcc
     3961 agtcgttgag cgcgctcttc tgcgcgtaca cgccgttggg accgcgtgcg ccgacgacgg
     4021 tgccgtcggg catgttgccg gccaggcggt gcaggtaagg ttcgcccgcc acaagcacca
     4081 gctggctgcg attgaacagc ccgcgcggcg tagcctgcag catgaaagcc tcgaggtcgc
     4141 cgccccagag actggagtga atcacatcgg agttggtttg cagcagggcg gagatttcgg
     4201 gaccgtactg tccggcgccg aacttgggca tctgcgaaga cgtgatcgtg gcttccggct
     4261 tgagtgttgc catcaccgcg gtgaagtccg cccacgaatc ctggccccag gcgtagttct
     4321 ggttgatgcc ggcgtagctc ttcaccgcgg gccggttttc cagcaggtac atcgcccccg
     4381 ccaccgcgtc catggtggcg tgcgagcggg tacggaatac atacttgtag ctcgcctcct
     4441 cgaacacgcg cggcgtgccg cagtcgaaca ggatggtgag ggtcttcaac tcttccgcga
     4501 ctggcgctac cgcgagacag tcgccgctgg agatatagcc gatgacgaag tcgacgttct
     4561 gccgctcgat caggttgcgc aactcggtca cctgcttggt ggcgccaccg gcttcgtcca
     4621 cgatcgtcag ttccagtggg gttccgccga aacccttgat gttgtaaggc gcgggcgcct
     4681 tgccggcgtt cagcgaatcg acaatcgcat cggcagccat cttggcgggc acgccgaagg
     4741 gtccggctgc cgggccggag aggaaggtga ccagcccgat gcggatcggc ttgtcctgtg
     4801 cgtgggcggt tgcgcaagta atggccagcg tcgcgcccgc aagcactgcg gcggccgttt
     4861 tcaggggccg cgaaaaattc cagtccatga tgtctcctcc ttcgtttttt tcatgcagca
     4921 aaaaataatc cagccggcca tgaaccgcgc tggactattc tgaatccagc ttgaatctag
     4981 cagcttgacc gttttcgggc aaggctgtat gcccgctcgc gccgggcccc gaatcaaccg
     5041 cgaatttcgc cgagtaatcc gtcgatggca gcctgtgccg gggcaagata agagctgccg
     5101 aacagattca ggtgattgag caaatggtac aggttgtaga gctgcttgcg tacccgatag
     5161 cccgggtcga gtggccacgc gtcgccgtag ccgcgataaa acgcctgagg aaagccgccg
     5221 aacagttcgg tcatggcgag gtcggcttcg cggtcgccga agtacaccgc gggatcgaag
     5281 atcgccggtt cgccgcccgc aagaaccgcg gcattgccgc cccacaggtc gccgtgcagc
     5341 agcgaagctt cgggcctgta gccggcaaag aatgcatcga gttcggcgag caacttctca
     5401 cccgaggatt gcagccggcc gcggtaagcg ttgcgctggg caagctgcaa ttgaaatcca
     5461 atccgccgct cacgcagaaa cacgatccag tcgctgcacc actcgttatg ctgcggcgtg
     5521 gagccgatgg tgttgtcccg actccagccg aagttggcgg cagtgctccg gtgcatcagg
     5581 gccaactgtg ctccgagtgc ggccaggccg tccgtgccat tgccgagttc gaggtgttcc
     5641 agcacgatcc aggaatggag agcatccgac ccgcagcaga tgggcgccgg cacgcgtacc
     5701 gcctttgtgg ctgcgagttc catgagcccc gccaactcag cctcgaacat ctccagccgc
     5761 caggcggaat tgagcttgac gaaaaagcgt cgttcgccgc cgtcaagccg gaatgcccgg
     5821 ttgatcgaac cgcccccggc gggctcggcg cgcgagccag ggaagggcga gcctgtgacg
     5881 cgggagagat cggcggcgat cgcccgccag gttgccgcgt cgggcgcggg caccacggca
     5941 tcctgcgcgg gatgcgctag cgcgcggcca gccaggcgcg ggcgcgggct atcgccgcgc
     6001 gtacctgggt cggcgctgtg ccgccgacgt gatcgcgcga agcgaccgag ccctcgagcg
     6061 agagcttgcc aattgcctca gtgtcgatca gcttggagaa ttgccgcaac tgctcgacgc
     6121 tcagttgcgt gaggtccaca cccttttcag cggcgaagcg caccgcgcgc gcgaccgcct
     6181 catgggcttc gcgaaacggc aggcccttgc ctaccagata gtcggccagg tcggttgccg
     6241 tggcgtagcc ctccagcagc gctgcgcgca tcgcctcctt gttgacggag atgccgccga
     6301 ccatgtcggc atagatcctg agcgttgcgg tcagcgtgtc caccgtgtcg aacagtggtt
     6361 ccttgtcttc ctggttgtcc ttgttgtagg ccagcggctg gcctttcatc agggtgagca
     6421 gagccaccag gtggccgttg acacgcccgg tcttgccgcg caccagttcc ggcacatcgg
     6481 ggtttttttt ctgcggcatg atcgacgagc cggtgcagaa ccggtcggcg agcgcgatga
     6541 atccgaagcg cggattcatc cacaggatca gttcttctga caaccgcgac agatgcgtca
     6601 tcgtcagcgc cgcgcaggcg cagaactcga tggcgaaatc gcgatccgac accgcatcga
     6661 gcgagttctc gcacacgccg tcgaaaccca attccctggc gacgccctcg cgatctatcg
     6721 ggtagtcggt gccggcgagc gccgccgcgc ccagaggcag cacgttgaac cgcctgcggc
     6781 agtccgcaaa gcgcgcggcg tcgcgcttca tcatctcgta gtaggccagc aggtggtgcg
     6841 cgaaactcac cggctgcgcc acctgcagat gcgtgtagcc gggcatgagc gtatcgacgt
     6901 gatgctcggc cagatcgagc aggcttttct ggaatgcgtg aatcagcgca aggatttcat
     6961 ccaccgccca gcgcagatag aggcggatgt cggttgcgac ctggtcgttg cgcgaacgcc
     7021 cggtatgcag ccgcttgccg gcattgccga ccaggtcggt gagccgcttc tcgatattca
     7081 gatgtacatc ctcaagatca accgaccagg taaacattcc ctggcggatc tccccgagga
     7141 tcgcgctcat gccgcgttcg atatcggcaa gatccttggc tgtgagcacg ccgaccgcat
     7201 gcagcatgcg cgcgtgcgcc agcgacgcgg cgatatcgaa ctccgcgagc cgcttgtcga
     7261 agtcgaccga tgcggtatag cgcttcacca agtccgcgac cggttcgctg aaccgtccgg
     7321 accgggtttt gccggcgtcg tccacgggtt tttccttggg ttgcattaag ctcctgccct
     7381 gttcacaatg actgcgttaa gcgcctgttc tggcgtgatt tttggcccga atcagccgat
     7441 ggcaagtata aatcaaaacc gtcaccccaa ccttcttcca aatttccgca atctgggcgt
     7501 catgttgcgc ctgctggtaa tggtcatagt gatgacggtt gttgcagctg tgctcaaatc
     7561 ctcgaactgg tacgacatgc tcgatcagtg gctgggcatt tccgcgctgg cgcagccgat
     7621 ggtcattctc tcattgctgc tgctcggcct cgccgatcgc tggctgcacg agctggatta
     7681 cccgctggcc gtcgccgtga tcgtggccat cgagctcggc attgtgtccg ttgtggtgat
     7741 cgctacctcg gcggtattca gtccggatac tccgggtttg gcacgcagtt gcttccttac
     7801 cgccgtggcc acgctgctcg tgctcgccta cttcaatttg cgcagccgga tgctgtcgcc
     7861 ggcgctgacc gaagcgagac tgcaggcgct gcaggcgcgc attcgcccgc attttctgtt
     7921 caacagcatc aatgcggtcc tgtcgctggt gcgca
//
LOCUS       DASQOP010000243         2651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1325815, whole genome shotgun sequence.
ACCESSION   DASQOP010000243 DASQOP010000000
VERSION     DASQOP010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325815"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VE030_09755"
     CDS             <1..217
                     /locus_tag="VE030_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23737.1"
                     /translation="LCDSKLAGFLRSTRLKMRPERAKRYNGAFFATNDLMQAFMTSTT
                     LVAIAGIGDNTLRSSLLRRRLMLAQPA"
     gene            286..858
                     /locus_tag="VE030_09760"
     CDS             286..858
                     /locus_tag="VE030_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HZF23738.1"
                     /translation="MEAFLTSTFLVAVAEIGDKTQLLSFVLAARLRKPWPIIAGILVA
                     TLANHALAGWAGSLLAGLVPQTPLIWIVGLSFIAFGLWALHPDALDGSPVIFKAGAFV
                     TTFVAFFLAEMGDKTQLATVALAARFDALAQVIVGTTLGMMIANVPAVWIGDRLAQQI
                     SMKAVRMLAAALFILVGVLTLWSTFASAWR"
     gene            858..1064
                     /locus_tag="VE030_09765"
     CDS             858..1064
                     /locus_tag="VE030_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich CWC family protein"
                     /protein_id="HZF23739.1"
                     /translation="MKNPVENRRCPDCGREFHCGIASDTPCWCATEFPPLMPPADAEA
                     CCYCRECLGKRIVARRESARKLLT"
     gene            complement(1155..1898)
                     /locus_tag="VE030_09770"
     CDS             complement(1155..1898)
                     /locus_tag="VE030_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HZF23740.1"
                     /translation="MDLSSPQFWIAVLQIILIDTLLSGDNAVVIALACRNLPPRQRRL
                     GVFWGVAGAIILRVTLTFFAVSLLAIPYLKIVGAALLLWIGVKLIQPQDDGEGQEIRA
                     GGNLLGAIRTIIIADFVMSLDNVIAVAAASRDSLFLLIFGLALSIPLMVWASQLVLRL
                     MDRFPSIIVLGGALLGWVGLSMAVHDPVVRDWVMAQAAWLNWTAPAAGAALVVAIGKL
                     LSLPAGPGRGRREGVVDLAERDRPVPPQS"
     gene            complement(1938..>2651)
                     /gene="sucD"
                     /locus_tag="VE030_09775"
     CDS             complement(1938..>2651)
                     /gene="sucD"
                     /locus_tag="VE030_09775"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HZF23741.1"
                     /translation="ASVREAKRQTGANASVIYVPPPGAAAAIWEAVEADLDLVICITE
                     GIPVRDMIEVRDRMRKQKRRTVLVGPNCPGVITPEEIKIGIMPGHIHRKGPVGVVSRS
                     GTLTYEAVGQLTELGLGQSSAVGIGGDPVNGLKHLDVLKLFNADPATEAVVMIGEIGG
                     TDEETAALWARDNMKKPIVGFIAGVTAPPGKRMGHAGAIISGGKGTAAEKLAVMEECG
                     IKVTRNPAEMGKLLKSVLR"
BASE COUNT          481 a          860 c          803 g          507 t
ORIGIN      
        1 tctgtgtgat tcaaaacttg caggtttcct ccggagtacg cgcttgaaaa tgcgcccgga
       61 gcgggcaaaa cgctacaatg gcgccttttt tgccacgaac gatctgatgc aggcattcat
      121 gacttcgaca acgttggtcg cgattgcggg aatcggcgac aacacgctgc gtagcagctt
      181 gttgcggcgc agactcatgc ttgcgcagcc agcgtgatgt cggaggcaca agacgcagtc
      241 ttagagccaa tctcgtccct atcgaagttt acaagaagtt tacaaatgga agcttttcta
      301 acctccactt ttctcgttgc agtggcagag attggcgata agactcagtt gctgtccttc
      361 gtgctcgccg cacgcctgcg caagccctgg ccgatcatcg ccggaatcct ggtcgcgaca
      421 ctggcgaatc acgcgctcgc gggctgggcc ggaagcctgc tcgccggcct ggtgccacag
      481 acgccgctga tatggatcgt gggtctgtcc ttcatcgcct tcggactctg ggctttgcac
      541 cccgacgcgc tcgacggctc gccggtgatt ttcaaggccg gggctttcgt caccactttt
      601 gtagccttct tcctcgccga gatgggagac aagactcagc tcgccaccgt ggcgctggcg
      661 gcgcgcttcg atgcgctggc gcaggtcatc gttggaacga cattgggaat gatgatcgcc
      721 aatgtcccgg cggtgtggat cggcgaccgg ctggcgcaac aaatctccat gaaggcagtg
      781 cgaatgcttg ccgccgcgct gttcatcctg gtcggggtgc tcacgctgtg gagcacgttc
      841 gcttcggcat ggcgctgatg aaaaatcccg tggagaaccg gcgctgtccg gactgcggcc
      901 gggagtttca ttgcggcatc gcgagtgaca cgccgtgctg gtgcgcgacc gagtttcctc
      961 cgctgatgcc gccggctgac gccgaagcct gctgctactg ccgggaatgc ctgggaaagc
     1021 ggatcgtcgc gcggcgggag tccgcgcgca agctcctcac ttgatcagca gcaccggatt
     1081 gtcggcgaga tggatgacct tggcagtcac caggccgtcc accggcagcg gaatcttcat
     1141 catcgctgcc tcattcagga ttggggcggc accgggcgat cccgttcggc gaggtccacc
     1201 acgccctcgc ggcggccgcg cccgggtccc gccggcagcg acagcaactt gccgatcgcc
     1261 accaccaggg ccgcgcctgc agccggtgcg gtccaattca gccatgcggc ctgtgccatc
     1321 acccagtccc tcacgacggg gtcatgcacc gccatcgaca gccccaccca tcccagcaaa
     1381 gcgccgccta atacgatgat cgacgggaag cgatccatca aacgcaggac caactggctc
     1441 gcccatacca tcagcgggat gctcaacgcc agtccgaaga tcagcagaaa caggctgtcg
     1501 cgcgacgcgg ccgccaccgc gatcacgttg tccaggctca tcacgaagtc ggcaatgatg
     1561 atggtgcgaa tggcaccgag caggtttccg ccagccctga tttcctgccc ctcgccgtcg
     1621 tcctgaggct ggatcagctt gacgccgatc cagagcagca aggcggcgcc gacaatcttg
     1681 agatagggaa tggccagcag gctcaccgca aagaacgtga gcgtcacccg caggatgatg
     1741 gcgccggcca cgccccagaa gacgccgagc ctgcgctggc gcggcggaag attccggcag
     1801 gccagcgcga tcaccaccgc attgtcgccg ctcagcaggg tgtcgatcag gatgatctgc
     1861 agaacggcga tccagaactg cggactgctc agatccatga agggttcgag tggattcgtt
     1921 gcggaaaaat cagacgctca acgcaaaacc gacttcagca gcttgcccat ctcggcgggg
     1981 ttgcgggtca ccttgatccc gcattcttcc atgaccgcga gtttctccgc cgccgtgccc
     2041 ttgccgccgg agatgatcgc accggcatgg cccatgcgct tgccgggagg ggcggtgacc
     2101 cccgcgatga agccgacgat cggtttcttc atgttgtctc tggcccacag cgccgcggtt
     2161 tcctcgtccg tgccgccgat ctcgccgatc atgaccaccg cttccgtcgc cggatcggcg
     2221 ttgaagagtt tgagcacatc gagatgtttg agtccgttga ccggatcgcc gccgatgcca
     2281 actgcggaag actggcccag tcccagctcg gtcagctgcc ccaccgcttc gtaggtgagc
     2341 gtcccggaac gggataccac accgaccggg cccttgcgat gaatatgtcc gggcatgatt
     2401 ccgatcttga tttcctccgg tgtgatcact cccgggcagt tcgggccgac caggacggtt
     2461 ctgcgtttct gtttgcgcat gcgatcgcgc acttcgatca tgtcgcgcac cgggatgcct
     2521 tcggtgatgc agatcaccag gtcgagatcg gcctcgaccg cttcccagat cgccgccgcc
     2581 gctcccggcg gaggcacata aatcaccgac gcattggcgc cggtctgccg tttcgcctcg
     2641 cgtaccgagg c
//
LOCUS       DASQOP010000244         4837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1925828, whole genome shotgun sequence.
ACCESSION   DASQOP010000244 DASQOP010000000
VERSION     DASQOP010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4837
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1925828"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="VE030_09780"
     CDS             complement(<1..870)
                     /locus_tag="VE030_09780"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HZF23742.1"
                     /translation="MMLRTRLCELLGIAHPIVCAPMGWITGPELTAAVSGAGGLAILG
                     AGANPPELLRRKIRRVRELTDRPFGVNLILSRPAEERFQVCIEERIPVLSLFWGDPAP
                     FVERAHDAGMKVLLQVGDVASAIDAKAKGVDAIIAQGVEAGGHVAGELSTMVLVPRVV
                     DAVAPVPVIAAGGVADARGLVAVLALGAQAAVLGTRFIATPEADTHPEYKRRILAAGG
                     EDTVRTTLFGGGWPNAPHRVLRTGFVEQWLAKPERGNENRPDEPVVAESRLGGEVFPI
                     RRFMVFPPNRETTG"
     gene            complement(896..1636)
                     /locus_tag="VE030_09785"
     CDS             complement(896..1636)
                     /locus_tag="VE030_09785"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF005068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HZF23743.1"
                     /translation="MKRFDPVTQARIDLTAALRSAARMGLNEGVCNHFSLEMPGDSGK
                     FLINPQGLHWSEITPADLMIVNEKGAVVEGKHTVEPTAFFIHGRIHLGKRKKCVLHTH
                     MPFATALTLLEGGELDVFANQNAMRYYGRIGYDREYGGVALDNAEGDRICNALGDNEV
                     VFMQHHGVIVCGDNVAYAFDDLYYLERSCMVQALAQGTGGKLKRVPEKMVAEAARQIG
                     GERQQSVLHFEALKRLLDRDEPGWSRLT"
     gene            complement(1754..2884)
                     /locus_tag="VE030_09790"
     CDS             complement(1754..2884)
                     /locus_tag="VE030_09790"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZF23744.1"
                     /translation="MAMHSNHEPAPRVLLVDPSPDDRLLMSLYLRRSGVEILTASNGE
                     EALQITAAKLPELVVLDLMMQGMSGFEVCKRIKSNPATALTPVVFLTLTDNREDRIKG
                     LQAGADDFFSKPLQRDEFLVRVKSLLATHRARKQEAAARQAMEADAPETPQGFERDVP
                     RRMMDRILATPEVNGQTPGGYQSRFNAVILFADLRGFTRMAEQLAPTQVVLLLNQYFS
                     LLTEVTYRYEGVIFNMAGDSLLVGFGVPFEQPDAAERAIKCGQDMLSAFETLSSEWHT
                     KYGLETGLGIGVNKGEVVAGNVGSSAYMSFTIIGDAVNIASRLMQRARAGELLLSERV
                     MLALLAARVKVDAIPLPPLTLRGRTEPIGLYCIAFKGRIDTR"
     gene            complement(3004..3483)
                     /gene="dtd"
                     /locus_tag="VE030_09795"
     CDS             complement(3004..3483)
                     /gene="dtd"
                     /locus_tag="VE030_09795"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HZF23745.1"
                     /translation="MIGLLQRVSEARVDVAGKTIGAIGTGLMVLVCAERGDTVTEAER
                     LLERLLGYRVFADAAGKMNLSLRDVAGGLLLVPQFTLAADTGKGTRPGFTPAAPPDVG
                     KRLFEHLLARARRAHSQVAAGEFGAMMKVSLINDGPVTLWLQVHPRSGSAGKVVLKE"
     gene            3595..4779
                     /locus_tag="VE030_09800"
     CDS             3595..4779
                     /locus_tag="VE030_09800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZF23746.1"
                     /translation="MKILMISDVYFPRVNGVSTSIQTFRRELATLGHQVDLIAPAYPD
                     PYAEDNRTLRVPSRYLPFDPEDRMMIPGAVRRLLPQLREAAYDLVHIQTPFVAHYLGM
                     EIADLLDIPRMETYHTFFEEYLFHYVPVVPKAALRALSRRFSRTQCNQMDALVVPSAA
                     MRDKLGDYGVRVPMHVVPTGIPPAQFAAGDGDAFRVAHGIPGKRPVLLFVGRVAFEKN
                     IDFLLRALERAVATISDLLLVIAGEGPALASLKRLAARLNLQKHTLFVGYLDRRGALL
                     DCYCAADAFVFASRTETQGLVLLEAMALGLPVISTAVMGTRDIVGPKRGALVPDDNEA
                     DFAEHIVRLMRDGDLRARLAEEGRAYVCEWHAGALARKLFATYQEVVATDQPARAASC
                     PA"
BASE COUNT          853 a         1578 c         1497 g          909 t
ORIGIN      
        1 accggtggtt tcgcggttgg gcggaaacac catgaagcgg cggatcggga aaacttcgcc
       61 gccgagccgg ctctcggcga ccaccggttc gtcgggccga ttctcattgc cgcgctctgg
      121 cttcgccagc cattgctcca cgaaaccggt acgcagcacc cggtgcggcg cgttcggcca
      181 gccgccaccg aacaacgtgg tgcgcacggt atcctccccg cccgcggcca ggatgcggcg
      241 cttgtattcc ggatgcgtat cggcttcggg agtggcgatg aagcgggtac cgagaacggc
      301 ggcctgtgcg cctagtgcaa gtacggcgac caggcctcgt gcgtcggcga ctccgcccgc
      361 ggcgatgacc ggaaccggcg ccaccgcatc caccacacgc ggcaccagta ccatggtcga
      421 gagttcaccg gcaacgtggc cgccagcctc caccccttgc gcgatgatcg cgtccacgcc
      481 tttggccttt gcatcgatcg cgctggcgac gtcgccaacc tgcaagagca ctttcatccc
      541 ggcgtcgtgg gcgcgctcta cgaaaggggc gggatcgccc cagaatagag aaagcaccgg
      601 aatcctttcc tcaatgcaga cctgaaagcg ctcttctgcg gggcgggaca ggatcagatt
      661 gacgccaaag ggccggtcgg taagttcgcg cacacggcga atcttccggc gcaacaactc
      721 gggcggattg gcgccggcgc cgagaatggc caggcctccg gccccgctca ccgccgcggt
      781 caattcgggc ccggtgatcc agcccatcgg cgcgcagacg atcggatgcg cgatgccgag
      841 cagctcacag agccgggtgc gcaacatcat caggcgttgc ccggggtcgg atcgatcagg
      901 tcagccgcga ccagcccggc tcgtcgcgat cgagcaggcg cttcagcgct tcgaagtgca
      961 ggacggactg ctggcgttcg ccgccgatct gccgcgccgc ttcggcgacc attttctccg
     1021 gcacgcgctt gagttttcca ccggtgcctt gcgccagcgc ctgcaccatg caggagcgct
     1081 cgagataata gaggtcgtcg aaggcataag cgacgttgtc gccgcagacg atcacgccgt
     1141 gatgctgcat gaagaccacc tcgttgtcgc caagcgcgtt gcagatgcga tcgccctcgg
     1201 cgttgtccag tgcgacgccg ccgtactcgc ggtcgtaacc gatgcggccg tagtagcgca
     1261 tggcgttctg gttggcgaac acgtcgagtt caccgccttc cagcagcgtc agcgcggtgg
     1321 cgaacggcat gtgagtatgc aggacgcact tcttgcgctt gccgagatga atgcggccgt
     1381 gaatgaagaa cgcggtcggc tcgaccgtat gcttgccttc gaccaccgcg cctttttcgt
     1441 tcacgatcat caggtccgca ggcgtgatct cggaccaatg aaggccctgg ggattaatca
     1501 ggaatttgcc ggaatcgccc ggcatttcca ggctgaagtg attacagacg ccctcgttca
     1561 gccccatgcg ggcagccgag cgcagcgccg cggtcaggtc tatcctggcc tgggtgaccg
     1621 gatcgaatcg tttcatgctg ttctccttag cttaggttct gtccattctg cactggaagt
     1681 ccgaatgccg gtgccgcggg ccgtggcaac gatgttcagc agttcagtca gcgtcagcgc
     1741 cgtcctgaag ccatcagcgc gtgtctatgc ggcccttgaa cgcgatgcaa tacaggccga
     1801 tcggttcggt ccgcccccgc agcgtcagcg gcggcagcgg aatcgcgtcc acttttactc
     1861 gtgccgccag cagcgccagc atgacccgct ccgagagcag caactccccc gcccgcgccc
     1921 gctgcatcag ccgcgaagcg atgttcaccg catcgccgat gatggtgaag ctcatgtacg
     1981 cggacgagcc gacgttgccg gccaccacct cgcccttgtt gacgccgata cccaacccgg
     2041 tctcgagccc gtatttcgta tgccactcgg atgacagggt ttcgaaggcg gagagcatat
     2101 cctgaccgca tttgatcgcg cgttcggcgg catccggctg ttcgaacgga accccgaatc
     2161 cgaccagcaa gctgtccccg gccatgttga agatcacgcc ctcgtagcga taggttactt
     2221 ccgtgagcag cgagaaatac tggttcagca gcagcacgac ctgcgtcggc gcaagttgtt
     2281 cggccatgcg ggtgaacccg cgcaagtcgg caaacaggat cacggcattg aaacgggact
     2341 ggtaacctcc cggtgtctgg ccgttgacct ccggcgtggc gaggatccta tccatcatcc
     2401 tgcgcggcac gtcgcgttcg aagccttgtg gcgtttccgg cgcatcggct tccatcgcct
     2461 gcctcgcggc tgcttcctgc ttgcgcgccc ggtgggtcgc aagcagtgac ttgacccgta
     2521 ccaggaactc gtcgcgctgt aacggcttgg aaaagaaatc gtcggcgccg gcctgcaagc
     2581 ccttgatgcg gtcttcgcgg ttgtcggtca aagtgaggaa gaccaccggc gtcagcgcgg
     2641 tcgctggatt ggatttgatg cgcttgcaga cttcgaagcc ggacatgccc tgcatcatca
     2701 ggtcgagcac caccagttcc ggtagtttcg ccgcggtaat ctgcagcgcc tcctcgccat
     2761 tgctggcggt gagaatctcc accccagagc gcctgagata gagcgacatc agcaggcggt
     2821 cgtcagggga agggtctacc agcagcacgc gcggggcggg ctcgtggttg gaatgcatcg
     2881 ccatcaggca tcgccgcgcc ggtcgggcgc aagggtttta agtaagctta ttattatctc
     2941 acgggcgttg cggcatccaa cgttgagaaa agggtggacg cgtcgcgacg aaagcgcttt
     3001 ctttcactcc ttgagcacaa ccttcccggc gctgccggaa cgcgggtgca cctgcagcca
     3061 gagcgtgacc ggaccgtcgt ttatcagaga cactttcatc atggcgccaa attcacctgc
     3121 cgcaacctga gaatgtgcgc ggcgggcgcg cgccagcaaa tgttcaaaca acctcttgcc
     3181 cacatcaggc ggggctgccg gagtgaagcc ggggcgcgtg ccctttccgg tatccgccgc
     3241 cagcgtgaac tgcggcacca gaagcagccc gcccgccacg tccctcaggc tcagattcat
     3301 cttgccggcg gcatcggcaa agactcgata gccgagcagt cgctccaaaa ggcgttccgc
     3361 ctcagtcacg gtatcgccgc gctcggcgca gaccagcacc atcagcccgg taccaatcgc
     3421 gccgatcgtc ttgccggcca cgtctacgcg tgcctcgctg acccgttgca gcagcccgat
     3481 cattgtttgc cggtttactt gtgttcacgg gattttacct gctgaccgcg ttttgtcttc
     3541 aatcggtcat gtcgccgtaa tatgctggct ggcatagcgt caacggatag aaacatgaaa
     3601 atactgatga tctcggacgt gtacttcccg cgggtgaacg gcgtatccac gtccatccag
     3661 acgttccgcc gcgagcttgc gacgctcggc catcaagtgg acctgatcgc gcccgcctac
     3721 cccgatccgt acgcggagga caaccgcacg ctgcgcgtgc cctcccggta tcttccgttc
     3781 gatccggaag accggatgat gatcccgggc gcagtgcgca ggttgttgcc gcagttgcgc
     3841 gaagctgcct atgacctggt ccatatccag acgccgttcg tcgctcatta cttgggcatg
     3901 gaaatcgccg acttgctgga catcccaagg atggagacct accacacttt tttcgaggag
     3961 taccttttcc attacgtgcc ggtggttcca aaagccgcgc tgcgggcgtt gtcgcgccgc
     4021 ttttcccgta cccagtgcaa tcagatggac gcgctggtgg tgccctcggc tgcgatgcgc
     4081 gacaagctgg gggactatgg cgtgcgcgta ccgatgcatg tcgttcccac cggcatcccg
     4141 cccgcacagt tcgccgccgg cgatggcgat gcctttcgcg tcgcgcatgg cattcccggc
     4201 aagcgcccgg tgttgttgtt cgtcggacgg gtggcgttcg agaaaaacat cgacttcctg
     4261 ctgcgggcgc tggagcgggc cgtcgccacc atttccgacc tgctgctggt aatcgccggc
     4321 gaggggccgg cgctggcatc gctgaagaga ctcgccgcga gactcaacct ccagaagcac
     4381 acattattcg tcggctacct cgacaggcgc ggcgccttgc tcgattgcta ctgcgccgcg
     4441 gacgccttcg tgttcgcctc gcgcacggag acccagggac tggtgctgct ggaggcgatg
     4501 gcgctcgggc ttccggtcat ctccaccgcc gtgatgggca cgcgcgacat cgtcgggccg
     4561 aagcgcggtg cgttggtgcc ggacgacaac gaagccgact tcgcagagca catcgtcaga
     4621 ttgatgcgcg acggcgatct gcgcgcgcgg ctggcagaag agggccgcgc ttacgtgtgc
     4681 gagtggcacg ccggtgcgct ggcgcgaaaa ctgttcgcca cgtaccagga agtcgtagcc
     4741 accgaccagc ccgcccgtgc cgcttcctgt ccggcatgag ggtcgggacg taagacccaa
     4801 cgactgccct cgaccactcg cgatgccgct acactgt
//
LOCUS       DASQOP010000245         4191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2537583, whole genome shotgun sequence.
ACCESSION   DASQOP010000245 DASQOP010000000
VERSION     DASQOP010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2537583"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..1259)
                     /locus_tag="VE030_09805"
     CDS             complement(198..1259)
                     /locus_tag="VE030_09805"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HZF23747.1"
                     /translation="MMAVIELDTPAVTVDLDVMEDNIRRVQAHLARHGIANRPHIKTH
                     KIPALGKMQMQAGAVGITCQKLGEVEVFTDAGVADDVLLTFNIVGDAKTDRLMALTKR
                     LRRLSVVLDSEAVAKGLSEAGVRHGADVCFLVECDTGMGRNGVQSPQATLDLARAAMK
                     LPRMRFEGIMTFPTRQPQSYQFFEEALALFKRAGIPVPVVSGGGTPAIFKAQEFPMLT
                     EHRAGTYIYNDVMTVASGTATWDNCAMRVRATVVSRPTGTRAVIDAGTKVLTSDQYTV
                     KGYGHVLECPEAVIAHLSEEHGVIDLSGCRERPAVGDIVHVIPNHCCVVSNMVDEVYG
                     VRGEQVEVTWPVAARGTVR"
     gene            complement(1256..1405)
                     /locus_tag="VE030_09810"
     CDS             complement(1256..1405)
                     /locus_tag="VE030_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23748.1"
                     /translation="MPPDLQKLVFEAARDGSAYERRRDVRAIESMKAEGTQMVIPERT
                     RKEIP"
     gene            complement(1856..2122)
                     /locus_tag="VE030_09815"
     CDS             complement(1856..2122)
                     /locus_tag="VE030_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23749.1"
                     /translation="MNHAVHNNAGMNFIGLFPLIPERVESRDRHPPGSESAAFPIQTC
                     GSGFPSAGGGAGASAGGGSFFSALRRRFSSCFLFFASSFWRFSN"
     gene            2082..2747
                     /locus_tag="VE030_09820"
     CDS             2082..2747
                     /locus_tag="VE030_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23750.1"
                     /translation="MKFIPALLCTAWFTVLCGGMAVAQDTKPAQDTKPAAITVLGDAS
                     PVMYGRSAKIKATVEAIDLDAREITLKGPKGRLITLRVKARARNLPQVQVGDEVIVRY
                     YESVGLQVRKTEASDAAPLEETTAGAEPGQTPASDAAKLFTIAAYVEAVSPKEKTLTL
                     KRPDGNLVDLYVRDPNVLESLTAGDNVVATYTEAAAVSIELPKKKEKPKPKKRRPTGA
                     SAQ"
     gene            complement(2796..3185)
                     /gene="msrB"
                     /locus_tag="VE030_09825"
     CDS             complement(2796..3185)
                     /gene="msrB"
                     /locus_tag="VE030_09825"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HZF23751.1"
                     /translation="MSEVQKSEQDWRKALTPEQYRVLREEGTERRFTSPLNSEYGKGT
                     FVCAGCGQPLFRSDMKFDSKTGWPSFFTAIPGSLETKVDRTLFMERTEYHCANCGGHQ
                     GHVFEDGPDPTGLRYCNNGVALKFEPD"
     gene            complement(3189..3506)
                     /locus_tag="VE030_09830"
     CDS             complement(3189..3506)
                     /locus_tag="VE030_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23752.1"
                     /translation="MISRIWHGWTSRHNADAYEKLLRGEIFRGIEQRDIRGYRGIHLL
                     RRDVSDGVEFVTVMWFDSPDSVRAFAGEDFEAAVVPPRARALLSRFDARSAHYDVIVA
                     PEQ"
     gene            3921..>4191
                     /locus_tag="VE030_09835"
     CDS             3921..>4191
                     /locus_tag="VE030_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23753.1"
                     /translation="MEFNILQHFKGLDELPKDPFNGNETGTSLFLTGGMGPKISQKMD
                     GYAKKAGMSRRNFIKSSLGFSAAMLAANEITGMKFFEVGAAEAADS"
BASE COUNT          849 a         1279 c         1287 g          776 t
ORIGIN      
        1 agaggacgcg gaggacgcag aggaaaggaa agagcaaaaa agtgttccac agttaaaaac
       61 tgatatcggt ggtcactccg gcacccggcc tgcttttact cccacatgtt ttcctcgttt
      121 tcggttttct tctgcgtcct ctgcggcctc tgcggcctcc gcggtgagcg ttgtactttc
      181 ctgacccttc agccaagcta acgcacggtg ccgcgcgccg cgaccggcca ggtcacctcg
      241 acctgctccc cgcgcacgcc atagacctcg tccaccatgt tgctcaccac gcagcagtgg
      301 ttgggtatga cgtggacgat gtcgccgacg gcgggtcgct cgcggcagcc cgaaaggtcg
      361 ataacaccgt gttcctccga gaggtgcgcg atcaccgcct ccgggcactc gagcacgtgg
      421 ccgtaaccct tgaccgtgta ctggtccgaa gtcagcacct tggtgccggc gtcgatgacc
      481 gcgcgcgtgc cggtagggcg gcttaccacc gtggcccgca cccgcatcgc gcagttgtcc
      541 caggtagcgg tgccggacgc caccgtcatc acgtcgttgt agatataggt cccggcgcgg
      601 tgctcggtga gcatcgggaa ctcctgcgcc ttgaagatgg cgggtgtgcc gccgccggaa
      661 acgaccggaa cgggaatgcc ggcgcgcttg aacagcgcca gcgcttcctc gaagaactga
      721 taggactgcg gctggcgggt ggggaaggtc ataatgccct cgaagcgcat gcgcggcagc
      781 ttcatcgccg cgcgcgccag gtccagcgtc gcctgagggg attgcacccc gttgcggccc
      841 atgccggtgt cgcactccac caggaagcac acgtccgcgc cgtgccgtac cccggcctcg
      901 gacagcccct tcgccaccgc ctcgctgtcg agcacgacgg agaggcgcct gaggcgcttg
      961 gtcaacgcca tcagccggtc ggttttcgcg tcaccgacga tgttgaacgt cagcaatacg
     1021 tcgtccgcga cgccggcgtc ggtgaacacc tcgacctcgc cgagtttctg gcaggtgatg
     1081 ccgaccgcgc cggcctgcat ctgcatcttg ccgagcgccg gaatcttatg cgtcttgatg
     1141 tgcgggcggt tggcgatgcc gtggcgcgcc aggtgcgcct gcacgcgacg gatgttgtcc
     1201 tccatcacgt cgaggtcgac ggtgacagcg ggcgtatcga gttcgatgac agccatcatg
     1261 gaatctcctt cctggtgcgc tcgggaatga ccatctgcgt gccttctgcc ttcatacttt
     1321 caatggcccg cacatcccgg cgccgttcgt aggcggagcc atcgcgcgcc gcctcgaaga
     1381 cgagcttctg caggtccggc ggcatccggg acgcccggtt ccagcgcctg gtaggcctcg
     1441 ccgagattca tcggcgtcgg gatagcgccg atggtatgat gaattatcta tccgcatgat
     1501 ccatatcact taaatgtttc gtcaggcggc tcgtccgcga aagcgcaacc gcctctcgca
     1561 cgcagttgag gagctgggca gccgaaccgg aacccgtgtc ctgggaccga tccactggaa
     1621 tctgctcgat cttgatgtca tgggctggcg ttataccgca atgctgcgat cgccatccgg
     1681 agcaggctcg tgagaacatg gcacagttgc agtcgagccc acgcgagttc ctggaacgcg
     1741 aactcgccgg cccggcggct gacggggtgg aggagccgca ggcggcggcg tgctgaggcg
     1801 gcatacgatt aacgaatttc tcttgaaagg accacagatt gccgaaacca aactatcaat
     1861 tcgagaagcg ccagaaagaa ctggcgaaaa agagaaagca ggaagaaaag cgcctgcgca
     1921 aggcggagaa gaacgagcca ccgccggctg atgctcccgc accgccgccg gctgaaggaa
     1981 agccagaccc gcaggtctga atcgggaaag cggcagattc agatccgggc ggatgccgat
     2041 cccggctctc gacgcgttca ggaatcaagg gaaacagtcc aatgaaattc attccggcat
     2101 tgttgtgcac cgcatggttc acggtactct gcggaggcat ggcagttgcc caagacacga
     2161 agcctgccca ggacacgaag cccgcagcca tcacggtgct gggagatgcc agtccggtca
     2221 tgtacgggcg gtcggcaaaa atcaaggcga cggtcgaggc gatcgatctg gatgctcgcg
     2281 agattacgtt gaaaggaccg aaaggccgcc tgatcacgct acgggtgaag gcgcgggccc
     2341 ggaatctgcc gcaggtccag gtaggcgacg aagtcatcgt gcgctattac gagtcggtgg
     2401 gattgcaggt acgcaagacg gaggctagcg atgcggcccc cctcgaggaa acaacagccg
     2461 gcgctgagcc tggacagaca ccggcttcgg acgcggcgaa actattcacc attgcagcct
     2521 acgttgaagc ggtaagtccg aaggagaaga cgctcaccct gaagcggccg gacggaaacc
     2581 tcgtcgacct ctacgtccgt gacccgaacg tactggaatc gctaaccgcg ggcgacaacg
     2641 tcgtcgccac gtacaccgag gcggctgcag tttcgatcga acttcccaag aagaaagaaa
     2701 agcccaagcc aaaaaagaga aggccaaccg gcgcctccgc gcagtgatcg tatcaggtag
     2761 tcaggcgcaa acaagttgtt gaaattgcca acccgtcaat caggctcgaa cttgagcgcc
     2821 acgccgttgt tgcagtaacg caggccggtc ggatcggggc catcttcgaa gacgtgcccc
     2881 tggtggccgc cgcagttcgc gcaatggtat tcggtgcgct ccatgaacaa cgtacgatcg
     2941 accttggttt ccagcgagcc gggaatcgcg gtgaaaaagc tgggccagcc ggtcttgctg
     3001 tcgaacttca tgtcggacct gaacaacggc tgcccgcagc cggcgcagac aaacgtgcct
     3061 ttgccgtatt cgctattgag cgggctggta aagcggcgct cggtgccttc ctcgcggagc
     3121 acgcggtact gctccggcgt cagcgccttg cgccaatctt gttccgattt ctggacttcg
     3181 ctcatctctt attgctccgg agcgacaata acatcgtaat gcgccgagcg tgcgtcgaac
     3241 cgggacagta gcgcgcgcgc ccttggcggc accaccgcgg cttcgaagtc ctctccggcg
     3301 aacgccctga ccgaatcggg cgaatcgaac cacatgacgg tcacgaattc gaccccgtca
     3361 gatacgtcac gtctgagcag atggatgccg cggtaacccc ggatatcacg ctgctcgatt
     3421 cccctgaaga tttcgccgcg aagcaacttt tcgtaggcgt cggcattgtg ccggctggtc
     3481 catccgtgcc agattctgct gatcatgggt gattccgtca gtcgtaggtt caaggttggc
     3541 agatgatcga atcctaatgc atccgcgtcg tcgcgggtgc tgcgctgccg catcacaaag
     3601 gccgtgttta ccgctgtcct ttgaacgagc ccgtctggca gcctccagcc ccgcgtgcta
     3661 cgccgaagcg tttgtttttc cgtgctttca aagtgccagg aaagattgaa ggaatctgtc
     3721 caccctcact aaccgttgtc cgggaaacgt ccgaccatca agtggccggg caggcgttgc
     3781 aacgcgcgtg cgagaagagt agcgtgaccg aaaacaagga ttgcattgtc ggtgtcggaa
     3841 tgagcggccg gcccggtatt tctggcgtgc cgttccgtgg tcaaaacaga aattttgata
     3901 acaagcggag gaggagcgcg atggagttca atatcctgca gcacttcaag ggccttgacg
     3961 agcttcccaa ggacccgttc aacggtaacg aaaccggcac ctcattgttc ctcaccgggg
     4021 gcatgggtcc taagatctcc cagaagatgg acggctatgc caagaaggcc ggcatgtccc
     4081 gacgcaattt catcaagtcg tccctgggtt tctcggcggc gatgctcgct gccaacgaaa
     4141 tcacgggaat gaagttcttc gaagtcggcg cggccgaggc tgccgactct g
//
LOCUS       DASQOP010000246         2140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1926613, whole genome shotgun sequence.
ACCESSION   DASQOP010000246 DASQOP010000000
VERSION     DASQOP010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1926613"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2108)
                     /locus_tag="VE030_09840"
     CDS             complement(<1..2108)
                     /locus_tag="VE030_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZF23754.1"
                     /translation="MATTLLTLGIALSGVMAFQLLPVSPLPQVDFPTISVQASLPGAS
                     PETMAATVATPLERALGRIAGITEMTSSSSLSSTRITLQFDLDRNIDGAARDVQAAIN
                     AARALLPTGLPSNPTYRKVNPADAPIMILSLTSESMTRGQMYDAASSILAQKLSQLKG
                     IGQVSVGGSSLPAVRVELLPSALHKYGIGTEDVRSAIAAANANRPKGMVEDGVRHWQI
                     YANDQARQAAEYLPLIIAYRNGAAVHLSDVAEITDSVQDLRNAGSADGKPAVLVILNR
                     QPGANIIATVNRVRAALPLLRASIPRAIDLAVVLDRTPTIRASLLEVERTLLISIALV
                     VMVVFLFLRKATATLIPTVAVPVSLLGTFGVMYLAGYSLNNLSLMALTVATGFVVDDA
                     IVVLENISRRIEEGVPPMEAALKGTREVGFTVLSMSLSLIAVFIPILLMGGIIGRLFR
                     EFAVTLSVAILVSLVLSLTTTPMMCARLLRPEKPEAEPGRLSRLSRRTFDSLLRGYER
                     SLAWALRHSRFMMLLLAATVCLNVYLYVIVPKGFFPQQDTGRLSGNIQADQGISFQAM
                     QKKLNDFIEIVRQDPAVENVIGFTGGGQRNSGQMFISLKPLSQRKMSADMVIARLRGK
                     LAREPGASLFLQPVQDIRMGGRAANAQYQYTLQADDLTELRAWEPRIRQALTELPQLA
                     DVNTDSQDKGIQTSLNIDRDA"
BASE COUNT          404 a          669 c          696 g          371 t
ORIGIN      
        1 gcgtcgcggt cgatgttcag agacgtctgt attcccttgt cctggctgtc ggtgttgacg
       61 tccgccagtt gcggcaactc ggtcagcgcc tggcggatgc gcggttccca cgcgcgcagt
      121 tcagtgagat cgtccgcctg cagcgtgtac tgatactgcg cgttggccgc gcgcccgccc
      181 atgcggatgt cctgaaccgg ctgcaggaac aggctggcgc cgggctcgcg cgcgagcttg
      241 ccgcgcaatc gcgcaatcac catgtcggcg gacatcttgc gctgcgaaag cggcttcagc
      301 gagatgaaca tctggccgct gttgcgctgc ccgccgccgg taaaaccaat gacgttttcc
      361 accgccgggt cctggcgcac gatttcgatg aagtcgttga gctttttctg catggcctgg
      421 aacgaaatgc cctggtcggc ctggatgttc ccgcttagac ggccggtatc ctgctgcgga
      481 aagaagccct tgggcacgat cacgtaaaga tagacattca ggcataccgt tgccgcgagc
      541 agcagcatca tgaagcggct gtgccgcaga gcccatgcga gcgaacgctc gtagccgcgc
      601 aaaagcgagt cgaaggttcg ccggctgagc cgcgacagcc ggccgggctc ggcctccggt
      661 ttttcgggcc gcaacaggcg cgcgcacatc atcggcgtcg tggtgagcga gagcaccagc
      721 gacaccagga tcgcgaccga cagcgtaacg gcgaattcgc ggaacagccg gccgatgatg
      781 ccgcccatca acaggatggg aatgaacacg gcgatcagcg acaggctcat cgacaacacg
      841 gtgaagccga cttcgcgcgt acccttgagt gcggcttcca tcggcggcac gccttcttcg
      901 atacggcggg agatgttctc cagcacgacg atggcatcgt ccacgacgaa acccgtggcc
      961 acggtcagcg ccatgagcga caggttattg aggctgtaac cggcgagata catgacgccg
     1021 aatgtgccga gcagggacac cggaacggcc actgtgggga tcagggtcgc ggtagctttg
     1081 cgcagaaaca ggaaaaccac catcaccacc agcgcaatgg agatcagcag agtacgttcg
     1141 acttcgagca gcgaggcgcg gatggtcggg gtacggtcca gcaccaccgc cagatcgatc
     1201 gcacggggaa tcgacgccct gagcaatggc agggccgccc ggacccggtt cacggtcgcg
     1261 attatgttcg cccccggctg gcgattcagg atgacgagta ctgccggctt gccgtcagcc
     1321 gacccggcat tgcgcaagtc ctgaaccgaa tccgttattt cggcgacgtc cgacagatgc
     1381 accgcggcgc cgttgcggta ggcgatgatc agcggcagat actcggcagc ttgcctggcc
     1441 tggtcgttgg cgtagatctg ccagtgccgt acgccgtcct ccaccatgcc cttgggccgg
     1501 ttcgcattcg cagcggcaat cgcgctacgc acgtcttccg tgccaatgcc gtacttgtgg
     1561 agcgcggacg gaagaagttc cacgcgtacc gccggcaggg aactgccgcc gactgaaacc
     1621 tggccgatgc ccttcagctg cgagagcttt tgcgcaagga tgctcgacgc cgcgtcatac
     1681 atctggccac gcgtcatgct ttccgacgtc agcgacagaa tcatgatcgg cgcatcggcc
     1741 ggattcacct tgcgataggt cgggttgctc ggcaatccgg tgggtagcag cgcgcgcgca
     1801 gcattgatcg ccgcctgtac gtcccgcgcc gcgccgtcga tgttgcgatc gaggtcgaat
     1861 tgcagcgtga tgcgggtcga gctgagcgaa ctcgacgacg tcatctcggt gatgccggcg
     1921 atgcggccca gggcgcgctc cagcggtgtc gccaccgttg cggccatggt ttccggactg
     1981 gcgccaggca gcgaggcctg caccgagatc gtcggaaagt ccacctgcgg caagggcgac
     2041 accggcagca gctggaacgc catgacgccg gacaacgcga tccccagcgt gagcagcgtg
     2101 gttgccactg gacggtggat gaagaactcg gagaacttca
//
LOCUS       DASQOP010000247         3739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_319830, whole genome shotgun sequence.
ACCESSION   DASQOP010000247 DASQOP010000000
VERSION     DASQOP010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319830"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /gene="cls"
                     /locus_tag="VE030_09845"
     CDS             <1..1015
                     /gene="cls"
                     /locus_tag="VE030_09845"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HZF23755.1"
                     /translation="YVLVQFYIIRDDDAGRELKARLLERARAGVRVHVLYDEIGSYEL
                     PRAYVNEMRASGISVRAFHAIGPGVNRFHINFRNHRKIVIVDGRVAWVGGLNVGDEYL
                     GRDPRIGYWRDTQLRVEGPVVQCVQVAFVEDWHWAAGELLDLNWDPRPAASGARRPIL
                     CLPSGPADELETCTLFFIAAIAEAKSRLWIASPYFVPDEQFVTALQLAALRGVEVRIL
                     MPERSNHPLVNLSGWSYIEELEKAGIAVYRHQHGFMHQKVTLIDDDYCTVGTANFDNR
                     SFRLNFEITMAVADRELAAQVRAMLENDFAHARRVTAAEFHSRSLAFRFAVRAARLSA
                     PVQ"
     gene            complement(1054..1617)
                     /locus_tag="VE030_09850"
     CDS             complement(1054..1617)
                     /locus_tag="VE030_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23756.1"
                     /translation="MATPNRKDPAQHWIVRFLPVETLVEAQKLAHRYREGEGVRLYVL
                     ERKWLVIPAALVMVLISIACAAGTVVFLAGAYSLLVLPALLLAPVILVGSLFVQMYVF
                     FYWLENRALTRALGHRAKPAHGTLAAWLSSKLGVDMGKLPPVPWILATVVLFAPLAML
                     AYFALNVALVLIVLAILMPILYARFDR"
     gene            1835..3739
                     /locus_tag="VE030_09855"
                     /pseudo
     CDS             1835..3739
                     /locus_tag="VE030_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762088.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
BASE COUNT          718 a         1148 c         1212 g          661 t
ORIGIN      
        1 atacgtgctg gtgcagttct acattatccg cgacgacgat gcggggcgcg agctcaaggc
       61 gcgcctgctt gagcgcgccc gcgccggcgt tcgcgtccat gtgctgtacg acgagatcgg
      121 tagctacgaa ctgccgcggg cctacgtcaa tgagatgcgc gcgagcggaa tctcggtgcg
      181 cgcgttccac gccatcggtc cgggagtgaa ccgcttccac atcaacttcc gtaaccacag
      241 gaaaatcgtg atcgtggacg gacgggtggc gtgggtgggc gggctgaacg tgggcgacga
      301 gtaccttggc cgtgatccgc gtatcggata ctggcgggat acgcaattgc gggtcgaagg
      361 gccggtcgtg cagtgcgtac aggtcgcatt cgtcgaagac tggcattggg ccgcgggcga
      421 attgctcgac ctgaactggg atccccggcc tgcggcgagc ggcgcccgcc gtcccatcct
      481 ctgcctcccc agcggcccag ccgacgagtt ggagacctgc acgctgtttt tcatcgcggc
      541 gatcgccgaa gcaaaatcac ggctgtggat cgccagcccg tacttcgttc cggacgaaca
      601 gtttgtcacg gcgctgcagt tggcggcgct gcgtggggtg gaggtgagga tcctgatgcc
      661 ggagcggtcc aaccacccgc tggtgaatct gtccggctgg tcgtatatcg aggagttgga
      721 gaaagccggg attgcggtct atcgtcacca acacggattc atgcaccaga aggtcacgct
      781 gatcgacgat gattactgca cggtgggcac cgccaatttc gacaatcgct cattccggct
      841 gaacttcgag atcaccatgg cggtggccga ccgcgaactg gcggcgcagg ttcgcgcgat
      901 gctggagaac gattttgctc acgcgcgccg tgtcacggcc gcggaattcc attcgcgctc
      961 tctggcattc cgcttcgccg ttcgcgcggc aaggctgtcc gcgccggtcc agtgagccgt
     1021 tgcgctctac ccgtcgtttc ccggtgaaca tggtcaccga tcaaaccgcg cataaagaat
     1081 cggcatcagg atcgctagaa cgatcaacac caaagcgacg ttcaacgcga aataggccag
     1141 catcgccagc ggcgcaaaca ggaccactgt cgcaaggatc cacggcaccg ggggtaactt
     1201 acccatgtcc acgcccagct tgctcgaaag ccacgccgca agcgttccgt gcgccggctt
     1261 ggcacgatgt ccgagcgctc gcgtcagggc acggttctcg agccagtaga agaacacata
     1321 catctggacg aacaggcttc cgaccaggat cacgggcgcc aggagtagcg ccggcagcac
     1381 aagaagcgag tacgctccgg caagaaacac cacggtcccc gcggcgcacg caatgctgat
     1441 gagtaccatg accagcgcgg ccggaatcac gagccacttt cgctcgagga cgtagagccg
     1501 gaccccctct ccttccctgt acctgtgcgc gagcttttgc gcctccacca gcgtctcaac
     1561 cggcaaaaat cgcacaatcc agtgctgcgc ggggtctttt cggtttgggg tcgccatgac
     1621 atgacctccc ttccgtaaga gacaacaagc atacgaaact ccgggcaaca atgctgagaa
     1681 tatttctaaa cctgcctcac atggaggaat tgctcgggca ctctgacgcc agcacgacga
     1741 tgatctacac tgacggcctt gaccggggcg ggctggcagt cgtcagcgcg ccgggcgcgg
     1801 tccgggggac atcgaactgc agctcgtaca atttttttct gattccggcc cgcttgcctc
     1861 ggccattccc ggctacggcc tgcgtccgca acaggtagag atggcgcagg cggtcgagca
     1921 ggcgatcgaa gagcgcagcg tcctgatcgc cgaggccggt acgggtaccg gcaagacgtt
     1981 cgcgtacctg gtgccggcgc ttgccaaggg cggcaaggtg atcatctcca ccggtaccaa
     2041 aaccctgcag gaccagttgt tcggccgcga catcccgacg gtgcgcaacg cgctgcaggc
     2101 gcccgtgacg gtcgcgctgc tcaagggtcg ggcgaattat gtctgccatt accacctggg
     2161 gcgcgccaag aacgatggtc gcttcctgga ccgcgacgat gtggtgtacc tggcgcgcat
     2221 cgagtcctat gccaggacca gcaagaccgg cgatcgcagc ggactggccg aagtgccgga
     2281 ggacgcgacc gtctggccgc aggtgacctc cacacgtgag aactgtctcg gcggcgattg
     2341 cccgaactac aagcagtgct tcgtcatgga ggcacgcaag caggcgatgg aagcggacgt
     2401 ggtggtggtg aatcatcacc tgttttttgc cgacgtggtg ctgcgcgacg agggtgtggc
     2461 ggagctgctg cccgcgtgca acaccgtgat cttcgacgag gctcatcaat tgccagagac
     2521 cgccagcttg ttcttcggcg aaaccatctc cacccatcag ctcatcgaac tcggacgcga
     2581 cacccggatg gaggccgccg cttcggcgaa ggacaatgcg gcgttgccgg atgccgcttc
     2641 gcaactggag aaagcggcgc gcgacctgcg gctgcaggtg cgcgatgaca tcggccgcca
     2701 gccttacgcg caggccgcgg cgaaccgcga cttcaccgac gcactgaatg cggcggcgac
     2761 acggctcacc gagtttgcag gggtgctgga agcgcaggcc gatcgctcgg agggcctccg
     2821 caactgttgg atgcggggga tggaaatgct ggaccgcctc ggccgctggc agggggaaga
     2881 ggatcgcgag cgggtgcgct gggtcgaggt gtttgcgcat tctgtgcacc tgaatgcgac
     2941 accgctctcg atcgcgggca tcttccatcg ccagatcagc ggccatccgc gcgcgtggat
     3001 ctttacttcc gcgacgctat cggtagccgg cgatttctcc cattaccagc gtgaaatggg
     3061 actggacgaa gcggccacag cattctggga aagtcccttc gattaccaga accaggcgct
     3121 gctttacgtc cccgagaaat tgccggaacc gaactccgag ggctacaccc gggcggtggt
     3181 ggacgcggcg ctgccagcga tcgaggcggc tgagggacgg accttcttcc tcttcaccag
     3241 tctcagggcc atgcgcgagg cgcgcgattt gatggaaacc ggcatgaagc gtatgggcat
     3301 ggagtatccg ctgctgatgc agggagaggg ctcgcgcacg gagctgctgg agcgcttccg
     3361 caaactcggt aatgcggtgc tgttggggag cgcatcgttc tgggaaggcg tggacgtcaa
     3421 aggcgaggcg ttgtcgctgg tgattatcga ccggttgccg tttgcccctc ccgacgatcc
     3481 ggtgctcgcc gcgcgcattg agaaaatgaa cagcgagggc cgaaacgcct tcatggagta
     3541 ccaggttccg caggcggtca tcacgttgaa gcagggctcg ggcagattga tccgcgacga
     3601 gaacgatcgc ggcgtgctga tgatctgcga cccgcgcctg atctccaagc actacggcaa
     3661 gcgcatctgg cgcagcctgc cgccgatgaa gcgcacccgg gatctcggcg aggtgcgcga
     3721 cttctacaag agcatcgcc
//
LOCUS       DASQOP010000248        19528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_525848, whole genome shotgun sequence.
ACCESSION   DASQOP010000248 DASQOP010000000
VERSION     DASQOP010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 19528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19528
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525848"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(242..1180)
                     /gene="xerC"
                     /locus_tag="VE030_09860"
     CDS             complement(242..1180)
                     /gene="xerC"
                     /locus_tag="VE030_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020830840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HZF23757.1"
                     /translation="MAGATVSPPLRGVAEPEHPYLAGFLSQLRQERRLSRHTADAYAR
                     DVRSLLELAGTTPLAELQIHQIRRFVARLHGRGLGGKSLARLLSAWRGFFNYLARDHG
                     FSSNPCTGVRAPKSPKSLPKALSPDEATRLVSITDDDKLAVRDRAIFEMFYSSGLRLS
                     ELTSLNPGDVSFADATVRVTGKGNKTRLVPVGSYALQALKAWMTVRELLAKQDETALF
                     VSQHGGAITPRAIQQRLKTWSIKQGLPGHVHPHMLRHSFASHVLQSSGDLRAVQEMLG
                     HSSISTTQVYTHLDFQYLAKVYDQAHPRAKRKTSKP"
     gene            complement(1181..1825)
                     /locus_tag="VE030_09865"
     CDS             complement(1181..1825)
                     /locus_tag="VE030_09865"
                     /inference="COORDINATES: protein motif:HMM:NF016243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HZF23758.1"
                     /translation="MSPEDVARYLQDHPEFFEEHAEVLAEIHIPHPHGGGAIPISERQ
                     IVSLREKTRVLEAKLRELLQFGEENDALGERVHRAAMELVKAPDLEAMLRVVYYNLRE
                     DFAVPHVSIRLWIEPDHPPLAEFGPASQEVRVFAESLNHPYCSSKPMFETGQWFSEAE
                     TIVKSFVYVPLRADRVFGLLILASEDPQRFYPEMGTLYLKRLGELVSTGLGRYL"
     gene            complement(1839..2669)
                     /gene="dapF"
                     /locus_tag="VE030_09870"
     CDS             complement(1839..2669)
                     /gene="dapF"
                     /locus_tag="VE030_09870"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HZF23759.1"
                     /translation="MRVKFTKMQGAGNDFVVVDATRQPLRLAPETIRKLAHRNFGVGF
                     DQMLVIEPARTPGTDFYYRVFNADGGEVNQCGNGARCFVRYVHDKGLSGKSAIRVETG
                     SGVIEPRLEKDGRVAVAMGAPEFEPAKIPFVAERRALTYALEVGGRSIEIGAVSMGNP
                     HAVQVVADVEAAPVLTEGPLIEKHPRFPQGVNAGYIEIHDRHRIRLRVFERGSGETLA
                     CGTGACAAVAVGIARGLLDSPVEVQTRGGKLEIAWQGAGHAVMMTGPAETVYEGEIIL
                     "
     gene            complement(2781..3314)
                     /locus_tag="VE030_09875"
     CDS             complement(2781..3314)
                     /locus_tag="VE030_09875"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZF23760.1"
                     /translation="MPAAPDDCRIAPLAIADIEPLTTLAHEIWHQHYPGIITVKQIEY
                     MLEQRYSPDTIRTEIESGAARWVKLLVNGELGGFASYEADGARRAVKLDKLYVHRQFR
                     GRGCGAALVRHVEQAADAQGCDKVYLQVNRNNFDSIAAYRRLGFEVMDRVKTDIGGGF
                     HMDDYVMAKEIRPEARG"
     gene            complement(3360..4229)
                     /locus_tag="VE030_09880"
     CDS             complement(3360..4229)
                     /locus_tag="VE030_09880"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HZF23761.1"
                     /translation="MLTRLAIGVFWLLHFLPLAVLARIGAVAGLAFMVFGRERREVAR
                     TNLALCFPGWDKARRKAVLRAHFRALGRSFVEAGIVWWGSEKRIRRIVRIEGEEYVRA
                     QGDRRVIFLVPHFAGIEMEGLRMSLDYKGMAVYSHQKNRVFDEFLVRVRSRFPGTRMA
                     ARQEGVKTLLRGFKDGLGLQLSPDLDLGPRDALFVPFFGVQAATVTALSRLARLANAV
                     IVPVVVRQLPGGEGYVLRMYPAWENYPGESVEADTRHMNAFIEQRILEMPEQYYWVHK
                     RFKTRPEGEPRIY"
     gene            complement(4229..5113)
                     /locus_tag="VE030_09885"
     CDS             complement(4229..5113)
                     /locus_tag="VE030_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZF23762.1"
                     /translation="MLIRLMRLLARAPLSWIHSAGAAIGWIVYLLSPSYAARLRENLL
                     QSGVWRDEAEYQRLLHQNIAESGKAGTELMPVWFRPASEARKLVVSVQSLALVEEAER
                     RGNGIIYLTPHLGCFDVAASWLAQRSPITVLYRPPKIQSLQPLIEVGRGRDGVHLAPV
                     SLSGVRRLLKALRRNEAVGILPDQVPGVGEGAWAEFFGRPAFTMTLVGRLVEATGATV
                     IMTAAERLPNGRGYSLDFILVEDDLSGQQGVRHLNAAIEQRVRRCPAQYLWSYNRYKV
                     PAGAPPPDAGAAAKGALH"
     gene            5198..6379
                     /gene="metK"
                     /locus_tag="VE030_09890"
     CDS             5198..6379
                     /gene="metK"
                     /locus_tag="VE030_09890"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006413300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HZF23763.1"
                     /translation="MSDFLFTSESVSEGHPDKVADQISDAVLDAILTQDKHARVAAET
                     LVNTGLVVMAGEITTNAVVDFQQVARDAIKRIGYDNTDYGIDYKGCAVLVAYDKQSPD
                     IKQGVDKAHDDPLNQGAGDQGLMFGYACDETPVLMPQAIHLAHRLVERQSELRRDGRL
                     PWLRPDAKSQVTIRYVNGKPDSIDTVVLSTQHSPDVSLEDIREAVVENIIKPVLPKNL
                     VKGKINYLVNPTGRFVVGGPQGDCGLTGRKIIVDSYGGAAHHGGGAFSGKDPSKVDRS
                     AAYAARYVAKNVVAAGLASKCEVQVSYAIGVAKPTSVMVTTFGTGKISDERIGQLIAK
                     HFDLRPRAIIQMLDLLRPIYEKTAAYGHFGRDEPEFTWENLDKVEALKADAGLKQTAA
                     A"
     regulatory      6687..6761
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            6822..8240
                     /gene="ahcY"
                     /locus_tag="VE030_09895"
     CDS             6822..8240
                     /gene="ahcY"
                     /locus_tag="VE030_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HZF23764.1"
                     /translation="MSAVPNSMKSADFHVADLSLADWGRKEIRIAETEMPGLMAIREE
                     YASTQPLRGARITGSLHMTIQTAVLIETLQALGARVRWASCNIYSTQDHAAAAIAATG
                     TPVFAYKGESLEEYWDYTHQIFEWPDGGFSNMILDDGGDATLLLHLGTRAETDSSVLK
                     NPGSEEETFLFAAIKARLARDPKWYSTRLVHIKGVTEETTTGVKRLYQMAKEGLLKFP
                     AINVNDSVTKSKFDNLYGCRESLVDGIKRATDVMIAGKIAVVAGYGDVGKGSAQALRA
                     LSAQVWVTEIDPICALQAAMEGYRVVTMDYAADQGDIFVTATGNFHVITHDHMRKMKN
                     QAIVCNIGHFDNEIDVAALKKYTWENIKPQVDHVIFPDGKRIILLAEGRLVNLGCATG
                     HPSYVMSSSFANQTIAQIELFNNPDKYRVGVYVLPKHLDEKVARLQLRKLAAQLTELT
                     DAQARYIGVRREGPYKPEHYRY"
     gene            8302..8655
                     /locus_tag="VE030_09900"
     CDS             8302..8655
                     /locus_tag="VE030_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018281122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HZF23765.1"
                     /translation="MKPTGHRDLRVWQDAMALVRVVYLVTQSFPADEQFGLSSQLRRA
                     AVSVPSNIAEGAARRSKREFAQFLSVARGSLAEVETQVLIARDLGYLVQDDDILAAVS
                     GLLRSISALMKSLER"
     gene            8665..9513
                     /gene="metF"
                     /locus_tag="VE030_09905"
     CDS             8665..9513
                     /gene="metF"
                     /locus_tag="VE030_09905"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HZF23766.1"
                     /translation="MSLVTRHPSPTFSFEFFPPKTPEGKEKLRATWRELAKLNPRFFS
                     VTFGAGGSTQQGTLETVLEIRAAGQDAAPHLSCVASSKTEIAAVLARYKDNGIRHVVA
                     LRGDLPSGIASSGELRYANDLVAFIRQTTGDWFQIEVACYPEYHPQTRNAADEMKNFK
                     RKVDAGANSAITQYFYNIDAYFRFVDECTVAGITVPIVPGIMPIGNFSQLARFSDACG
                     AEIPRWMRIKLESFRDDTTSIRAFGLDVVTALCEKLLASGAPGLHFYTMNQADATTTI
                     WQRLGL"
     gene            complement(9784..11028)
                     /locus_tag="VE030_09910"
     CDS             complement(9784..11028)
                     /locus_tag="VE030_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HZF23767.1"
                     /translation="MSSQDYAPKKKISSFYPPQRHLMGPGPSEIHPRVFSAMGKPTIG
                     YLDPVFVEMMEELKESLRYAFQTNNALTFPVSGPGSVGMEMCFVNMINPGDKVIVCRN
                     GVFGGRMIENVQRCGGMAVVVEDRWGTPVDPQKVEDAFKANADAKILAFVHAETSTGV
                     QSDARTLAELARKHGALTIMDAVTSLGGTPVLTDEWGIDAIYSASQKCLSCTPGLSPI
                     SFSDRVVEMVKNRKEKVHSWFMDVNLLLGYWSATNRTYHHTAPTNSLYALHEALLMLR
                     EEGLENAWARHQRNHKALKAGLEMLGLNYLVEEPYRLPQMNAVTVLPGVDEREVRRRL
                     LLDYNLEIGAGLGHLAGKIWRFGIMGYSCKMENVMLSLCALETVFADLGLKVEFGAAE
                     AAAYHAYAALPNKVQSVRAVAA"
     gene            complement(11166..11588)
                     /locus_tag="VE030_09915"
     CDS             complement(11166..11588)
                     /locus_tag="VE030_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012733751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HZF23768.1"
                     /translation="MRRAIVAFVCGLVFGAGLIISQMSNPAKVIGFLDVTGNWDPSLA
                     LVMAGAIAVFGVLYRLALRQGTPLLAPQFTLPEKDSLDAPLMVGALIFGVGWGLGGFC
                     PGPAIVSAAFGDARVWVFLAAMIAGMLLYRIGLRRTHD"
     gene            complement(11585..12010)
                     /locus_tag="VE030_09920"
     CDS             complement(11585..12010)
                     /locus_tag="VE030_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HZF23769.1"
                     /translation="MTVVNFTPWSALTGGALIGLAAAGMWWLLGRLAGVSGILGTALI
                     SRGEDFTWRAAFIAGLFLAGVAAAVLLPGEVQFNLEAGYGQVLLAGALVGLGTQMGSG
                     CTSGHGVCGVSRLSPRSIVATLLFMVAGMVTVYFWVHQP"
     gene            complement(12007..12897)
                     /gene="ttcA"
                     /locus_tag="VE030_09925"
     CDS             complement(12007..12897)
                     /gene="ttcA"
                     /locus_tag="VE030_09925"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014723781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HZF23770.1"
                     /translation="MATLQKQVYEGNKLAKRLRRQVGEAIADFGMIRAGDKVMVCLSG
                     GKDSYALLDILLSLKQHAPIDFEIVAVNLDQKHPGFPAEVLPEYLTAVGVPFRIIEQD
                     TYSVVKRVIPEGKTMCSLCSRLRRGALYRVAKEIGATRIALGHHRDDILETFFLNLFH
                     GGRLKAMPPKLVSDDGAHIVIRPLAYCKEQDLEEYAKIRAFPIIPCNLCGSQENLQRQ
                     VVKDMMREWQKKFPGRLETMFASLQRVSPSHLLDAQQFDFKNLKTQSDAYLDGDIAFD
                     EEDLSAKLLQLPISKAAESK"
     gene            complement(12911..13288)
                     /locus_tag="VE030_09930"
     CDS             complement(12911..13288)
                     /locus_tag="VE030_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HZF23771.1"
                     /translation="MAGKLGRARRIFLKNFETRISMGIHDFEKKARQRVMVNVDLYLE
                     PDARVERDHISETVDYDFLRREIAALADSGHFHLQETFCERILAICLAKSGVVAVRVS
                     SEKPDVYPDCESIGFEIFRAKQD"
     gene            complement(13329..14126)
                     /locus_tag="VE030_09935"
     CDS             complement(13329..14126)
                     /locus_tag="VE030_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZF23772.1"
                     /translation="MQLQTRMAQVKRKQQVALVTGAARRIGRAIAIGLADDGWDVAVH
                     YHRSKHDAETLANEIAARGVKAVAVGCDLEDVNALSKLLPECGRRLGPVGCLVNNAAR
                     FEYDNIESLDARKFERHVNVNLRAPLLLARDFAKQLPESVVGCIVNMLDQKVFNLNPD
                     FLSYTVAKIGLEGATRVLAMSMAPRVRVCGIAPGITLVSGEQSEEGFERAHSFAPLGR
                     SSDLEDVVNAVRFVVSAKSLTGETIVVDGGQHLWPRPRDVQFEVAKG"
     gene            14210..15388
                     /locus_tag="VE030_09940"
     CDS             14210..15388
                     /locus_tag="VE030_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZF23773.1"
                     /translation="MQAVPLNRSAAELPVPSDDARRHSDRLVDEIRAEVQRHGGWISF
                     ARYMEMALYAPGLGYYVAGTAKLGAGGDFVTAPEVSPLFGRTLAGQVAEILAHTGGGV
                     LELGAGSGVLALSLLEELQACNRLPDRYRILEVSPELVQRQRRLLTQRLPGLVDRVEW
                     IASLPQRFTGVMLANEVLDALPVHLFAWRDEGVLERGVTWKGEGIDWAERSPGPGAAR
                     DAASALRIEVPYQSEVSVAVPALVRTLAGALDRGVLLCVDYGFGRREYYHPQRHRGTL
                     MCHYRHRAHDDPFFFPGLQDITAHVDFTSVAEAGIESGLQVLGYTTQAQFLINCGIAD
                     LLARTPAEQAATYLPLAAGVQQLISPAEMGDLFKVIALGRGVQPPIAGFRSGDKSRLL
                     "
     gene            15712..17055
                     /gene="mpl"
                     /locus_tag="VE030_09945"
     CDS             15712..17055
                     /gene="mpl"
                     /locus_tag="VE030_09945"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HZF23774.1"
                     /translation="MHIHILGICGTFMGGIAVIARQAGHKVTGCDANVYPPMSTQLQA
                     QGIELIEGFDPAQIELEPDLFVIGNVVSRGNPLMEEILNRGLAFASGPQWLYENVLRG
                     KWVLAVAGTHGKTTATSMLAWILEHAGLNPGFLIGGVPINFEISARATDSAFFIIEAD
                     EYDTAFFDKRSKFIHYMPRTAILNNLEFDHADIFPDVAAIETQFHHLVRTIPGNGLII
                     ANGREQSMKRVLARGVWSKVELLGVDEGWVARSRDEHAFDVFFEGGPRGTVNWGLLGE
                     HNRHNALAALAAARHAGVPVTKGIEALAEFRNVKRRLEVRGTVNDITIYDDFAHHPTA
                     IETTIAGLRSKVNGARILAVLEPRSNTMKMGVMKDALAGSLAGADLVFCYTNGLGWDA
                     TPVLAPLGARSACFDDLRKLTEAIVAASRPGDHVLVMSNGGFGGIHAMLLEALKH"
     gene            complement(17257..18555)
                     /locus_tag="VE030_09950"
     CDS             complement(17257..18555)
                     /locus_tag="VE030_09950"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HZF23775.1"
                     /translation="MSGKWMVLAIAAGAALALQADDDVSRRDQLRQVPARVFPGRVHL
                     LPATLDTVQWGWFDNSEPPVLTIKPGDTVVMETMMHSHNQIVPGVEIEQIKSLRVDNP
                     GRGPHSVTGPVYIEGAEPGDVLKVRINRIVPRAYGANFNLPGMLGQFPGEFPEGQVRY
                     FYLDTQRMVTEFAAGIEIPLAPFPGILGVARAEPGQYSTVPPGPFGGNMDIRELKEGT
                     TLYVPVFVPGALLWSGDSHAAQGNGEVNLTALETAFKELSLTVDVVKRRSLEWPRIET
                     PTHWITMGADPDLNQALAILFDETAKFLSEQRGISTHAARGLIPLVSDCRIAEVVNHS
                     KALYCMNAKDWRTVKLPPLAARETPRNFITVGADADLQKAMDTASMSMIEVLQQKKGL
                     TRLDAYSLASLTMDCRLGNPAAEVQRVHCFVPKNLWIGKR"
     gene            18757..19320
                     /locus_tag="VE030_09955"
     CDS             18757..19320
                     /locus_tag="VE030_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HZF23776.1"
                     /translation="MIVYIHGFNSSPASHKSNQLRERLSALGRGAEFVCPALPHWPKQ
                     AMALLEREVARVPADGVTLVGSSLGGFYATALVEKTGVRAVLVNPAITPQEGLRAYLG
                     PQRNLYSGEEYVLTEEHLGQLAELQVRKPARLDRYLLMHTTGDELLDWRIAVDHYRGC
                     RQVVVQGSDHGFKEFGDYLDIVLEFAK"
BASE COUNT         3728 a         6120 c         6013 g         3667 t
ORIGIN      
        1 cacggatgcc ggcgcaggag cgacttctcg cggccgggct tcagtatgag tttgtgcata
       61 cttcaaaaag aatcctgaac cacggaggac acagaggaca cggaggaaaa ccaaaaacga
      121 ggaaaaacat gcgagagtta aaaacaggtc cggtgccgaa gtgaccacca ataccgggtt
      181 tcaactttcg aatgcttttt tgctcttgcc cttcctccgt gtcctctgtg tcctccgtgg
      241 ttcaaggttt agaggtcttg cgtttcgcgc gcgggtgcgc ctgatcgtag accttcgcaa
      301 ggtactgaaa atccaaatgc gtgtacacct gcgtggtgga gatgctggag tgacccagca
      361 tttcctgtac cgcgcgcagg tcgccgctgg attgcagcac gtgcgaggcg aacgagtggc
      421 gcagcatgtg cggatggacg tgcccgggga gcccctgctt gatactccag gttttcaggc
      481 gttgctggat tgcgcgcggc gtgatggcac cgccgtgctg gctgacgaac aacgcggtct
      541 cgtcctgttt cgccagcaac tcccgcaccg tcatccaggc cttcaacgct tgcagtgcgt
      601 agctgccgac cggcaccagc ctggtcttgt tgcctttgcc agtcacgcgc accgtggcat
      661 ccgcgaaact gacgtcgcca ggattgagcg aagtcagctc cgaaagccgc aggcccgaag
      721 agtagaacat ctcgaagatc gcccggtcgc gtaccgccag cttgtcgtcg tcagtaatgc
      781 tgacaaggcg ggtggcttcg tctggcgaca gcgccttggg cagcgacttt ggagacttgg
      841 gcgcccgcac gccggtgcag ggattggagc tgaacccgtg atcgcgcgcc agatagttga
      901 aaaatccgcg ccaggccgac aacaggcgtg ccagcgattt gccgccaagc ccgcgcccgt
      961 gcagccgggc cacgaagcgg cggatctggt gaatctgcag ttccgcgagc ggcgtcgtcc
     1021 ccgccagttc gagtagcgag cgcacgtcgc gcgcgtacgc atcggcggtg tggcgcgaca
     1081 aacgccgttc ctgccgcaac tgggacagga aaccggcaag gtacggatgc tcgggctcag
     1141 cgaccccacg gaggggcggg gaaactgttg cgccggccat ttacagatat ctgccgagtc
     1201 cggtgctgac cagctcaccg agccgcttaa ggtacaacgt gcccatttcg gggtagaagc
     1261 gctgcgggtc ttcgctggcg agtatcaaca ggccgaacac tctgtctgcc cgcagcggca
     1321 cgtagacaaa cgacttcacg atggtttccg cttcgctgaa ccactgtccg gtttcgaaca
     1381 tcggtttgct gctgcaatat ggatgattga ggctttcggc gaaaacccgc acctcctggc
     1441 tcgctggtcc gaattcggcg agaggcggat gatccggttc gatccaaagg cgaatgctca
     1501 catgcggcac ggcaaagtct tcgcgcaggt tgtagtagac caccctcaac atcgcctcga
     1561 ggtccggggc cttgaccagt tccatggccg cgcgatgcac ccgctcgccg agcgcatcgt
     1621 tttcctcgcc gaattgcagc agttcccgca gcttcgcctc gaggaccctg gttttctcac
     1681 gcaggctgac gatctggcgc tcggaaatcg gtatcgcgcc gccgccatgc gggtgcggaa
     1741 tatggatttc cgccagcact tcggcatgct cttcgaagaa ttccgggtga tcctgtaggt
     1801 agcgggcaac gtcttctggg ctcattccga atctttcgtt acaggatgat ttcaccttcg
     1861 taaacggttt cagccggccc ggtcatcatc accgcatggc ctgccccctg ccatgcgatt
     1921 tcgagtttgc cgccgcgggt ctgtacttcc accggggagt cgagcagacc gcgcgctatc
     1981 cctaccgcga ctgcggcgca ggcgccggtg ccgcaagcga gcgtttcccc gcttccccgc
     2041 tcgaaaaccc gcagtctgat gcggtgccgg tcgtggatct cgatgtagcc ggcattcacc
     2101 ccttgcggga aacgtggatg cttttcgatc aatggcccct cggtcagcac tggcgcggcc
     2161 tcgacgtcag ccaccacctg caccgcatgc ggattgccca tggacacggc gccgatttcg
     2221 atagacctgc cgccgacttc aagtgcatag gttagcgcgc gacgttctgc cacaaacgga
     2281 atcttcgcgg gctcgaactc cggcgcaccc atcgccaccg ctacccgccc gtctttttca
     2341 agccgcggct cgattacgcc cgagccggtt tccactcgga tcgccgactt gccggacagt
     2401 cccttgtcgt gcacgtaacg cacgaagcag cgtgcgccgt tgccgcattg attcacctcg
     2461 ccgccgtcgg cgttgaagac gcggtagtag aagtcggttc cgggtgtgcg cgccggctcg
     2521 atcaccagca tctggtcgaa tccgacgccg aagttgcgat gcgccagctt gcggatggtc
     2581 tccggtgcca gacgcagcgg ttggcgggtg gcgtcgacaa ccacgaagtc gttcccggcg
     2641 ccttgcatct tggtgaactt gactcgcaca agaggttaga ggcgagaggc gagaggcgag
     2701 aggaaaaccc gaaacgtacg aagctgcggt acgttcggtt tgaagggggt gagcgccatg
     2761 gcttgcagtt cctcttgcct ctagcctctg gcctctggcc tgatttcctt tgccatcacg
     2821 taatcgtcca tgtggaaacc gccgccgatg tctgtcttga ctctgtccat cacttcaaac
     2881 ccgaggcgcc ggtaagccgc tatcgagtcg aaattgttcc tgttgacctg caggtacacc
     2941 ttatcgcagc cctgggcgtc cgcggcctgc tcgacatgcc ggaccagcgc tgctccacaa
     3001 cccctgccgc ggaactggcg gtgcacgtac agtttgtcga gcttcacggc gcgccgcgcg
     3061 ccgtccgcct cgtaagaagc gaatccgccg agttcgccat tcaccagcag cttgacccac
     3121 cgggccgcac cgctctcgat ctcggtgcga atcgtatcag gcgagtagcg ctgctcaagc
     3181 atatattcta tctgcttgac cgtgatgata ccgggatagt gctggtgcca gatctcgtgc
     3241 gccagcgtgg tgagcggttc gatgtccgca atcgcgagcg gagcaatgcg gcagtcgtcc
     3301 ggagccgcgg gcatcacaat gcggaaacgt cgaagccgtt ccgagcgggt tgattcatct
     3361 caatagatcc tcggttcacc ttccgggcgc gtcttgaacc gcttgtgcac ccagtagtac
     3421 tgctcgggca tctccaggat cctctgttcg atgaatgcat tcatgtggcg tgtgtcggct
     3481 tccacgcttt cgccgggata attctcccag gccggataca tgcgcaacac gtagccttcg
     3541 ccgccgggca actgacgcac caccaccggc acgatgacgg cattggcaag ccgggcgagc
     3601 cgcgacagtg ccgtgacggt tgccgcctgt acgccgaaga acggcacgaa tagggcgtcg
     3661 cgcggaccta gatcgaggtc gggcgaaagt tgcaggccca gaccgtcctt gaacccgcgc
     3721 agcagcgtct tgacaccctc ctgccgcgcc gccatgcgcg tgcccggaaa acggctgcgc
     3781 acgcgcacga ggaattcatc gaagacgcgg ttcttctggt ggctgtaaac cgccatgccc
     3841 ttgtagtcca gcgacatgcg cagtccttcc atctcgatgc cggcgaaatg cggcaccagg
     3901 aagatgacgc gccggtcgcc ctgggcgcgt acgtactcct cgccctcgat gcgcacgatg
     3961 cgccggatgc gcttttcaga accccaccag acgatacccg cctcgacaaa acttctgccg
     4021 agagcacgga aatgagcccg caacacggcc ttgcggcgtg ctttgtccca tcccggaaaa
     4081 cacagcgcca gattggttcg cgccacttcc cgtcgctcgc gaccgaagac catgaatgcg
     4141 agaccggcga ccgccccgat acgcgccaga actgcgaggg gcagaaaatg cagcagccag
     4201 aacacgccaa tggcaaggcg agtcagcatc agtgcagcgc gcctttcgca gcagcgcctg
     4261 cgtcaggagg tggggcaccg gccggcacct tataacggtt gtagctccac aggtactgcg
     4321 cggggcagcg ccggacccgc tgctcgatcg cggcattgag atgacgcacg ccctgctggc
     4381 ccgacaggtc atcttcgacg aggatgaaat cgagtgaata gccgcggcca ttcggcagcc
     4441 gttccgcggc ggtcatgatg acggttgcgc cggtggcttc gacgagccgc ccgaccaggg
     4501 tcatcgtaaa tgcgggtcgc ccgaagaatt ccgcccacgc gccttcgcca actccgggca
     4561 cctgatcggg taggatgccg accgcttcgt tgcgcctgag ggccttgagc agtcggcgca
     4621 cgccgctgag actgaccggt gccaggtgca cgccgtcccg gccgcgcccg acttcgatga
     4681 gcggctgcaa gctttgtatc ttcggcggcc ggtacagcac ggtgattgga ctgcgttgtg
     4741 ccaaccacga ggcggcaacg tcgaaacaac cgagatgcgg cgtcagatag atgatcccgt
     4801 tgccgcgccg ttccgcttcc tcgaccagcg ctagcgactg aacggacacg accaatttgc
     4861 gcgcttcaga agctggacga aaccatacgg gcatcagctc ggttccagcc ttgccggatt
     4921 cggcaatgtt ctggtggagc aggcgctgat actcggcttc atcgcgccag acgccgctct
     4981 gcagcaggtt ctcgcgcaga cgcgcagcgt acgagggaga aaggagatag acgatccaac
     5041 cgattgcggc gcccgccgag tgaatccagg acagcggcgc tctggcaagc agccgcatta
     5101 accttatgag catgaactta ttcggtttct cgctttgttc ttttggcgtg tttttgctat
     5161 cctgacaacc ttcccggatc gaatgcaagg cttacgaatg agcgacttcc tgtttacgtc
     5221 cgagtcggta tccgaaggtc atcccgacaa agtggccgac cagatttccg atgcagttct
     5281 ggacgccatc ttaacccagg acaagcatgc ccgcgtggcg gcggagacgc tggtcaatac
     5341 cggtctggtg gtgatggccg gcgagatcac caccaatgcg gtggtggatt tccagcaggt
     5401 tgcgcgcgac gctatcaagc gtatcggcta cgacaacacc gactacggca tcgactacaa
     5461 gggatgcgcg gtgcttgtgg cgtacgacaa gcaaagccct gacatcaagc agggcgtgga
     5521 caaggcccat gacgatccgc tcaatcaggg ggccggcgac caaggcctga tgttcgggta
     5581 cgcctgcgac gaaaccccgg tgctgatgcc gcaggcgatc caccttgcgc accggctggt
     5641 ggagcgccag tcggagttgc gccgcgacgg ccgtctgccg tggctgcgtc ccgacgccaa
     5701 gtcgcaggtg acaatccgct acgtgaacgg caagcccgac tcgatcgata ccgtggtgct
     5761 gtccacgcag cattcccccg acgtttcgct cgaagatatc cgcgaagcag tggtggaaaa
     5821 tatcatcaag ccggtgctac ccaagaatct ggtcaagggc aagatcaact atctggtgaa
     5881 cccgaccggc cgcttcgtgg tcggcggacc gcagggggat tgtggactga ccggccgcaa
     5941 gatcatcgtg gacagttacg gcggcgccgc ccatcacggc ggcggcgcat tctccgggaa
     6001 ggatccgtcc aaagtcgacc gctcggccgc atacgcggcg cgttatgtcg cgaagaacgt
     6061 cgttgcggcg gggctggcca gcaagtgcga agtccaggtg tcctacgcga tcggggtggc
     6121 gaaacccacc agcgtgatgg tcaccacctt cggcaccgga aaaatatccg acgagaggat
     6181 cggccaattg atcgccaagc atttcgacct gcgtcccaga gcgatcatcc agatgctcga
     6241 tctgctgcgc ccgatctacg aaaagaccgc cgcctacggg catttcggcc gcgacgagcc
     6301 ggagttcacc tgggagaatc tggacaaagt ggaagccctg aaagcggatg cggggctgaa
     6361 acagactgca gcggcctgac cggccgcctt aggagcgctg acgcagtaga cggggagaag
     6421 ggctggatcg ggataagaaa ccaagggatc gcacagtgcg gtcccttttg tttttccggg
     6481 ttctgcatgc acagccgcag cggcctgccc ggccgctata gaagcgttga cgcaaaggaa
     6541 cgccaaggac gcaaaggaag gctggaacga ggatcaaaaa aagggatcgc ccgatgtggt
     6601 cccttttgcc ttcggggttc tgcatgcttg atcgcagcgt cccaaagggg cacttttcat
     6661 gtaaccgctg ccggcctata atttgccctg ctgaggagcg ttgcgacccg ccccacgagg
     6721 acaggccagg ctcggcaagg aaataacggc gctcacaaac ctttgattgg tttggagcgc
     6781 cgttgtcatt tcggcgctcc gttttgactg ggagagtgtc gatgtctgcc gtcccgaatt
     6841 ccatgaagtc cgccgatttc cacgtcgccg acctgtcgct cgccgactgg ggccgcaagg
     6901 aaatccgcat tgccgaaacc gagatgcccg gtctgatggc gatccgcgag gagtacgcct
     6961 cgacccagcc gctgcgcggt gcgcgcatca ccggctcgct gcacatgacc atccagacag
     7021 cggtgctcat cgagacgctg caggcactgg gtgcccgggt gcgctgggcc tcgtgcaata
     7081 tctactcgac ccaggaccac gcggccgcgg ccatcgccgc gaccggtacg ccggtgtttg
     7141 cctacaaggg cgaatcgctg gaggagtact gggactacac ccaccaaatt ttcgaatggc
     7201 cggacggcgg tttttcgaac atgatcctcg acgacggcgg cgacgcgacg ctgcttctgc
     7261 acctgggcac gcgcgccgag accgattcat cggtgctgaa gaatccgggc agcgaggaag
     7321 agacattcct gttcgccgcc atcaaggcca ggctcgcccg ggacccgaag tggtattcca
     7381 cgcggctcgt gcacatcaag ggcgtgaccg aggagaccac cacgggcgtc aagcgcctgt
     7441 accagatggc caaggagggg ctcctcaagt tcccggcgat aaatgtcaac gattccgtca
     7501 ccaagtccaa gttcgacaat ctctacggct gccgggagtc gctggttgac ggcatcaagc
     7561 gcgccaccga cgtcatgatc gccggcaaga tcgcggtggt cgcgggctac ggcgacgtgg
     7621 gcaagggctc ggcgcaggcg ttgcgggcgc tttcggcgca ggtgtgggtg acggaaatcg
     7681 atccgatctg cgcgctgcag gcagctatgg aaggctatcg cgtggtgacc atggactacg
     7741 ccgcggacca gggcgacatc ttcgtcaccg ccaccggcaa tttccatgtg atcacccatg
     7801 atcacatgag gaagatgaag aaccaggcga tcgtctgcaa catcggccat ttcgataacg
     7861 aaatcgatgt tgccgcgctc aagaagtaca cctgggagaa catcaagccg caggtggacc
     7921 acgtcatatt cccggacggc aaacgcatca tcctgctggc cgaagggcgg ctggtaaatc
     7981 tcggctgcgc caccgggcat ccgagctacg tgatgtcctc gtcattcgcc aaccaaacca
     8041 tcgcgcagat cgagctgttc aacaatcccg acaagtaccg ggtgggcgtg tacgtcctgc
     8101 ccaagcatct cgacgagaag gttgcgcggc tgcaactgcg gaaactggcg gcgcaactca
     8161 ccgaactgac cgatgcgcag gcgcgctata tcggcgtcag gagggaaggt ccgtacaagc
     8221 cggagcacta ccgttactga gcggcgtggc cgcttagtaa cgggtgacaa atgacaggtg
     8281 acgggtgacg gggaaaggca gttgaaaccg acaggccatc gcgatttgcg tgtatggcaa
     8341 gatgcgatgg cgctggtacg ggttgtctac ctcgttaccc aaagctttcc ggccgacgag
     8401 cagttcggat tgagttcgca attgagacgg gcggctgtat cggtgccctc caatatcgcg
     8461 gaaggagctg cgcgcagaag caagcgggag ttcgcgcaat tcctctctgt ggcccgaggt
     8521 tctctcgccg aagtggaaac ccaggtcctc attgcgagag atttgggata tctggtgcaa
     8581 gacgatgaca ttctcgccgc tgtttcagga ctgctgcgct ccatctctgc gctcatgaaa
     8641 tcgctcgaga gataaggatc tgtcatgtca ctcgtcactc gtcacccgtc accgactttc
     8701 agtttcgaat tcttcccgcc gaagacgccg gagggaaagg aaaagctgcg cgccacgtgg
     8761 cgggagctgg cgaagctcaa tccacgtttt ttctcggtga ccttcggcgc tggaggttcc
     8821 acccagcaag gcacgctcga aaccgtgctc gagatccgcg cggcgggcca ggacgcggcg
     8881 ccgcacctgt cctgcgtggc ctccagcaaa accgaaattg ccgcagtcct ggcgcgctac
     8941 aaggacaacg gtatccgcca cgtcgtggcc ctgcgcggtg acctgccctc gggcatcgcc
     9001 tccagcggcg agttgagata tgcgaatgac ctggtggcat tcattcgcca gaccaccggc
     9061 gactggttcc agatagaagt ggcctgctac cccgagtacc acccgcagac acgcaacgcc
     9121 gccgacgaga tgaagaactt caagcgcaag gtggacgccg gcgccaactc ggcgatcacg
     9181 cagtatttct acaatatcga cgcctatttc cgttttgtcg acgaatgcac ggtcgcgggc
     9241 atcacggtcc ccatcgttcc cggcatcatg ccgatcggaa atttttcgca actggcgaga
     9301 ttttccgacg cctgcggtgc ggagatcccg cgctggatgc gcatcaagct cgagagtttc
     9361 cgcgacgata ccacttcgat ccgcgccttc ggcctggatg tggtcacggc gttatgcgag
     9421 aagctgcttg cctcgggcgc gccggggttg cacttttaca cgatgaacca ggccgacgcg
     9481 accaccacga tctggcagcg ccttggctta taggtgaggc gtcaagcgaa aggcgtgagg
     9541 cgcaaaatca gcatgatcac caggcgacac tagaatcctg agtagcggga aaggcgtttt
     9601 caccgcaaag acgcaaagat cgcaaagaaa tgcagaaaat ggacggggag aacgtgcatt
     9661 tggcaaaacc cgtcttggtc gtcagtatcg gctatccatg tgtcgaacaa aatgcacagc
     9721 tctttttttc gttgtccgct gtttctttgc gttctttgcg tctttgcggt gaaaaggttt
     9781 atttcaggcg gcgacggcgc gcaccgactg cactttgttc ggaagcgccg cgtaggcgtg
     9841 gtaagcggca gcctcggcgg cgccgaattc cactttcaag ccgagatccg caaacaccgt
     9901 ttccagcgcg catagcgaca gcatcacgtt ctccatcttg caggagtaac ccatgatgcc
     9961 gaagcgccag atcttgccgg cgagatggcc caggccggcg ccgatctcca ggttgtaatc
    10021 cagcagcagc cggcggcgta cttccctttc atcaactccc ggcagcacgg tcactgcgtt
    10081 catctgcggc aggcggtagg gttcttcgac caggtagttc aatcccagca tctccaggcc
    10141 ggccttgagc gccttgtgat tgcgctggtg gcgcgcccat gcgttctcca ggccttcctc
    10201 gcgcaacatc agcagcgctt cgtgcagcgc gtacagcgaa ttggtcggcg cagtgtggtg
    10261 ataggtgcga ttagtcgcgc tccagtagcc gagcaggagg ttcacatcca tgaaccagct
    10321 gtgcaccttt tccttgcggt tcttcaccat ctcgaccacg cggtcgctga agctgattgg
    10381 cgatagcccc ggcgtgcagg acaggcactt ctggctggcg gaatagatcg catcgattcc
    10441 ccattcgtcg gtcagtaccg gagtgccgcc gagcgaggta accgcgtcca tgatggtgag
    10501 cgcgccgtgt ttgcgggcga gttcggcgag cgtcctggcg tccgattgca cgccggtcga
    10561 ggtttcggcg tgcacgaagg cgaggatttt ggcatccgcg ttggccttga acgcgtcctc
    10621 gactttttgc ggatccaccg gcgtgcccca acggtcttcc accaccacag ccatgccgcc
    10681 gcagcgctgg acgttctcga tcatgcggcc gccgaacacg ccgttgcggc agacgatgac
    10741 cttgtcgccg ggattgatca tattgacgaa gcacatttcc atccccaccg atccagggcc
    10801 ggaaaccggg aaggtcagcg cgttgttggt ctgaaaggcg tagcgcaggg actccttcag
    10861 ctcctccatc atttccacga acacgggatc gagatagccg atagtcggct tgcccatcgc
    10921 cgaaaacacc cgtggatgga tttccgaggg tcccggtccc atcagatggc gttgcggcgg
    10981 atagaaggaa gaaatcttct tcttcggtgc gtagtcctgg ctggacatgg tttccccggc
    11041 aggagagttg gattcatagc ccgcgctcgg tgcaagaaaa cctggcgcag aaagacgcgc
    11101 agtgtagcaa ggtcccggcg cacgggccaa gctacgttgc gcaggaattg agcaggccga
    11161 acggctcagt cgtgggtccg gcgcagcccg atgcggtaaa gaagcatgcc ggcgatcata
    11221 gccgcaagga agacccagac tcgcgcatcg ccgaaggcag cgcttactat agccggaccc
    11281 ggacagaacc cgcccagccc ccagccgacg ccgaagatga gcgcgccgac catcaggggc
    11341 gcatccaggc tgtccttctc cggaagagtg aactgggggg caagcagcgg cgtcccctgg
    11401 cgcaacgcca ggcgatacag cacgccgaac accgcgatcg cgccggccat taccagcgcc
    11461 agactcgggt cccaattgcc ggtgacatcg agaaagccga tcacctttgc cggattactc
    11521 atctgcgaaa tgatcagccc ggcgccaaac accaggccgc agacgaaggc gacgatggcg
    11581 cgcctcacgg ctggtgtacc cagaaataaa cggtgaccat ccccgccacc atgaacagca
    11641 gcgtcgccac gattgaacgc ggcgacaggc gcgacacgcc gcacacgcca tgaccgctgg
    11701 tgcagccgct acccatctgc gtgccgaggc ccaccagggc gccggcgagc agcacctggc
    11761 cgtaaccggc ttcgaggtta aactgcacct cgccgggtaa caagacggcg gcggcgactc
    11821 cggcgagaaa cagtccggca atgaaagccg cccgccaggt gaaatcctct ccgcgcgaaa
    11881 tcaacgccgt gccgaggatg ccgctgacgc cggccaggcg cccgagcaac caccacatgc
    11941 cggcggcagc cagaccgatc agggcgccgc cggtcagcgc cgaccacggc gtgaaattga
    12001 cgacggtcat ttgctttccg cggccttgct gatcggaagc tgcaaaagct ttgccgagag
    12061 gtcctcctcg tcgaaggcga tgtcgccgtc gagataggcg tccgactggg tcttgaggtt
    12121 cttgaaatcg aactgttgcg cgtcgagcag atgcgagggc gaaacccgct gcaggctggc
    12181 gaacatggtc tccaggcggc cggggaattt cttttgccac tcgcgcatca tgtccttgac
    12241 cacctggcgc tgcaggtttt cctgcgagcc gcagagattg caggggatga tcgggaacgc
    12301 gcggatcttc gcatattcct ccaggtcctg ttccttgcag taggccagcg ggcgaatgac
    12361 gatgtgggcg ccgtcatcgc tcaccagctt gggcggcatg gccttgagcc gtccgccgtg
    12421 aaagagattg aggaaaaaag tctccaggat gtcgtcgcgg tgatgcccca gggcgatgcg
    12481 cgttgcgccg atttcttttg ccactcggta gagcgcgccg cggcgcagcc gcgagcacag
    12541 cgagcacatg gtcttgccct cggggatgac gcgcttgacc acgctgtagg tgtcctgctc
    12601 gatgatccgg aacggcacgc cgaccgcggt caggtactcg ggcagcacct cggcggggaa
    12661 tcccgggtgc ttctgatcga ggttgaccgc gacgatttcg aaatcgatgg gtgcgtgttg
    12721 cttgagcgac agcaggatgt cgagcagcgc gtagctgtcc ttgccgcccg acaggcagac
    12781 catgaccttg tcgccagcgc ggatcatgcc gaaatcggca atggcttcgc cgacctggcg
    12841 gcgcagccgc ttggcgagct tgttgccttc gtaaacctgt ttctgcagtg ttgccatcta
    12901 gtcctgcttc ctagtcctgc ttcgcgcgga aaatctcgaa accgatcgat tcgcagtcgg
    12961 ggtacacgtc cggcttctcg ctggacaccc gcaccgccac cacccccgac ttggccaggc
    13021 agatcgccag gattctctcg caaaacgtct cctgcaggtg aaagtggccg ctgtcggcca
    13081 gcgcggcgat ctcgcggcgc aggaaatcat agtccacggt ttcgctgatg tggtcgcgct
    13141 cgaccctggc atccggctcg agatacagat ccacgttgac cattacccgc tgccgggctt
    13201 tcttctcgaa gtcgtggatt cccatcgaga tccgggtctc gaagttcttc aagaaaatgc
    13261 ggcgggcccg gcccaacttg cccgccatat ccactgccga acgcgggtgc agcgtcgccg
    13321 ccgtcatgtc agccctttgc cacttcgaac tggacgtcac gcgggcgcgg ccacaggtgc
    13381 tggccgccgt cgacgacgat ggtttcgccc gtcagcgatt ttgcgctcac cacgaacctt
    13441 accgcattca ccacgtcctc caggtcggag ctgcggccca gaggcgcaaa gctatgcgcg
    13501 cgttcgaagc cctcctcgct ttgctcgcca ctcaccagcg tgatacccgg ggcgatgccg
    13561 catacccgca cccgtggcgc catcgacatc gccaacaccc gggtcgcccc ttcgaggccg
    13621 atcttggcaa cggtgtagga caggaaatcc gggttcaggt tgaacacttt ctggtccagc
    13681 atgttgacga tacagcccac cacactctcc ggcagttgct ttgcgaaatc ccgtgccagc
    13741 agcaagggtg cgcgcaggtt cacgttcaca tgccgttcaa acttccttgc gtccaggctt
    13801 tcgatattgt cgtactcgaa ccgcgccgcg ttgttcacca ggcagccgac cggcccgagc
    13861 cggcggccgc attcgggaag gagtttcgac aaggcgttca cgtcttcgag gtcgcagccg
    13921 accgcgaccg ccttcacgcc tcgcgccgca atttcgttcg ccagcgtttc ggcgtcgtgc
    13981 ttcgaccgat ggtaatggac cgccacgtcc cagccgtcat cggccagtcc gatcgcgatc
    14041 gcgcggccga tccgcctggc ggcaccggtc actaatgcaa cctgttgctt gcgcttgacc
    14101 tgagccatcc tggtctgcag ttgcacgggt tgccccgtgc gatcttgaat agttgtccgg
    14161 cgcgttgttg aatagcgatt gtagcttaac cgcccctcga tcccgcccgg tgcaagccgt
    14221 tccgcttaac cgatccgccg ccgaactgcc cgtcccatcc gatgacgcac gccgtcacag
    14281 cgatcggctg gtcgatgaga tccgcgcgga agtccaaaga cacggcggct ggatctcctt
    14341 cgcccgctac atggagatgg cgctatacgc gccggggctg ggttattacg tcgccggaac
    14401 cgcgaaactc ggagccgggg gggatttcgt aaccgcgccc gaagtgtccc cattgttcgg
    14461 acgcacgctt gcgggccagg tcgcggagat ccttgcgcac accggaggcg gggtgctgga
    14521 actgggtgcg ggatcgggcg tgctggccct gtccctgctg gaagagttgc aagcgtgcaa
    14581 ccgccttccg gaccggtatc ggattcttga agtcagcccg gagctggtac agcggcaacg
    14641 ccggctgctg acgcagcgac tgcccgggct tgtcgacagg gtcgaatgga ttgcgtcgtt
    14701 gccgcagcga ttcaccggcg tgatgctggc gaacgaagtg ctggatgcgc tgccggtaca
    14761 tctgttcgcg tggcgcgacg aaggcgtgct cgagcgcggc gtaacctgga aaggcgaagg
    14821 aatcgactgg gcggaacgca gcccgggccc gggcgcggcg cgggatgccg catcggcgtt
    14881 gcggatcgaa gtaccgtacc agagcgaagt atccgtcgct gtgccggcgc tggtacgcac
    14941 gctggccggc gcgctcgacc gaggcgtact gttgtgcgtg gactacggct tcggccgccg
    15001 cgagtattat catccgcagc gtcacagggg gacgctcatg tgccactacc gccatcgcgc
    15061 gcacgacgat cccttcttct ttcccgggct gcaggacatc actgcgcacg tggactttac
    15121 ctcggtggcc gaagccggta tcgaatccgg actgcaagtg ctcggataca ccacgcaggc
    15181 acagttcctg atcaattgcg gcatcgccga cctgctcgct cgcacgcctg ccgaacaggc
    15241 tgcgacttat ctgccgctgg ccgccggcgt gcagcaactg atctcgccgg cggaaatggg
    15301 cgatctgttc aaggtgatcg cgctcggccg cggcgttcag ccgccaatcg ccggtttcag
    15361 gagcggcgac aagagccgct tgctctgagg acccggtacc cggcgcgtgg gcgatgcagg
    15421 cgttgcgccg acacatccgc ggctgctccg ctgcgctcga agcagccgag gccggccact
    15481 tcgcccccga gcggggcggt agaattgctg ccgccgcgct ggtggcgttt gctgattctg
    15541 gacactgagg acgactcacc acagagacac agagtcacag agaagatcga gacagggaag
    15601 aacaaggcag tcgttccagt agtttggtct cttgcctgcg gccgagaccg gttgctcatt
    15661 ccggttttgc ttttctccgc gcctctgtgt ctctgtggtg agacattcat aatgcatatc
    15721 catatcctag gcatctgcgg cacgttcatg ggcgggatcg cggtgatcgc caggcaggcg
    15781 gggcacaagg tgaccggctg cgacgcgaat gtctatccgc cgatgagcac gcagttgcag
    15841 gcgcagggca tcgagctgat cgagggcttc gatccggcgc agatcgaact cgagcccgat
    15901 ctgttcgtga tcggcaacgt ggtgtcgcgc ggcaatccgt tgatggaaga aatcctcaac
    15961 cgcggactcg cgttcgcttc cgggccgcag tggttgtacg agaacgtatt gcggggaaag
    16021 tgggtactgg cggtcgccgg cacccacggc aagaccaccg ccacgtcgat gctggcgtgg
    16081 attctggagc acgccgggct caatccggga ttcctgatcg gcggcgtgcc gatcaatttc
    16141 gagatttccg cgcgcgccac cgattcggca ttctttatca tcgaggcgga cgaatacgac
    16201 accgcgttct tcgacaaacg ctccaagttc atccactaca tgccgcgtac tgcgatcctg
    16261 aacaacctcg agttcgatca cgccgatatc tttcccgacg tggcggcgat cgaaacccag
    16321 ttccaccacc tggtgcggac catccccggc aacggcctga tcatcgccaa cggccgcgag
    16381 cagagcatga agcgcgtcct ggcacgcggc gtctggagca aggtggaact gctgggcgtg
    16441 gacgagggct gggtcgccag gtcgcgcgac gagcacgcct tcgacgtgtt cttcgagggc
    16501 ggaccgcgcg gcacagtgaa ctggggtttg ctgggcgagc acaaccgcca caacgcgctg
    16561 gcggcgcttg ccgcggcacg ccacgccgga gtgccggtca ccaaaggcat cgaggcgctg
    16621 gcagagttca ggaacgtgaa acgccgcctg gaagtgcgcg gcacagtcaa cgacatcacc
    16681 atatacgacg acttcgccca ccatcccacc gcgatcgaga ccaccatcgc gggtctgcgc
    16741 agcaaggtga atggcgcccg catccttgcc gtcctggaac cgcgctcgaa caccatgaag
    16801 atgggcgtga tgaaggacgc gctggcgggg agtctcgccg gggccgatct ggtgttctgc
    16861 tataccaacg ggctgggctg ggatgccacg ccggtactcg cgccgctcgg cgcaagatcg
    16921 gcctgcttcg acgatctgcg aaagctcacg gaagcgatcg tcgccgccag ccgtccgggc
    16981 gaccacgtgc tggtaatgag caacggcggc ttcggcggca ttcacgccat gcttctggaa
    17041 gcgctcaagc actagctggc tattgcgggc ccggaaatac agcgccaacc acggaggaca
    17101 cagaggacac ggaggaaaaa caaaagcaaa agtagaagag tgtaaaaacc ttgcgccggt
    17161 ggtcacgttg tcaacagacc gaaaatctac attgcgcctt tttttgacct tgctccgtgt
    17221 cctctgtgtc ctccatagct caagatttct ccctggctac cgtttgccga tccagagatt
    17281 tttcggtacg aagcagtgca cgcgctgcac ttccgccgca gggttgccta agcggcagtc
    17341 catggtcagg ctggcaagcg agtaggcgtc cagtcgcgtc aacccctttt tctgttgcag
    17401 cacctcgatc atgctcatgg aggccgtgtc catcgccttc tgcagatcgg cgtctgcgcc
    17461 cacggtgatg aaattgcgcg gcgtctcgcg cgccgcaagc ggcggcagtt tcaccgtcct
    17521 ccagtccttc gcgttcatgc agtagagcgc tttgctgtgg ttgacgactt ccgcaatcct
    17581 gcagtcggac accagcggaa tgagcccgcg cgcggcgtgc gtgctgatgc cgcgctgttc
    17641 ggagagaaac ttcgcggttt catcgaacag gattgccagc gcctgattca ggtccgggtc
    17701 ggcacccatg gtgatccagt gcgttggggt ttcgatgcgc ggccactcga gggacctccg
    17761 cttgaccacg tcaaccgtga gtgacagttc cttgaatgca gtctcgagcg cagtcaggtt
    17821 gacttcgccg ttgccctgcg cggcgtgcga atcccccgac cagagtagcg cgccggggac
    17881 gaacaccggt acgtacagcg tggtaccttc cttcagctcg cggatgtcca tgtttccgcc
    17941 gaaaggtccg ggcggcacgg tgctgtactg ccccggttcg gcgcgcgcca ccccgaggat
    18001 gccggggaaa ggcgccagcg ggatttcgat ccccgcggcg aactcggtca ccatgcgctg
    18061 ggtatcgaga taaaagtagc gcacctggcc ttccgggaac tcgcccggga actggccgag
    18121 catgcccggc agattgaagt tggcgccgta ggcacgcggc acgatgcggt tgatacggac
    18181 cttgagcacg tcccccggct cagcgccttc gatatacacc ggcccggtta ccgagtgcgg
    18241 cccgcgtccg gggttgtcga cgcgcaacga cttgatctgc tcgatctcca ctcccgggac
    18301 gatctgattg tgggagtgca tcatggtttc catcaccacc gtatcgcccg gcttgatcgt
    18361 gagtaccggc ggttcgctgt tgtcgaacca gccccattgc acggtatcga gcgtggcagg
    18421 caatagatgg acccgcccgg ggaagaccct tgccggcacc tgcctcagtt gatcgcgccg
    18481 ggagacgtca tcgtccgcct gtagcgcgag tgccgcgccg gctgcaatcg ccagaaccat
    18541 ccatttcccg ctcatcgaac ttctccttcc gtgtgctcgt tgaatcgcgg ccctttcgag
    18601 agcataccgc tttgcaatcg ccgtaccacc agggggattg caaaactgca gcggcggccg
    18661 aggctggctg gccccgacac ccgcccgcgc cgggtgtttt ttgtgcgtag tgtggccgtc
    18721 gggcacaata ccggggcgtc cgtttccgca aacgtcatga tcgtctatat ccacggattc
    18781 aacagttccc ccgcatcaca caaatccaat cagctgcgcg agcgcctttc cgcgctcggt
    18841 cgcggcgcgg agttcgtctg cccggccttg ccgcactggc cgaaacaggc gatggcgcta
    18901 ctggagcgcg aagttgcgcg cgtgccggca gacggggtca ctctcgtcgg cagttcgctc
    18961 ggcggtttct acgccacggc cctcgtcgag aaaaccggcg tgcgcgctgt gctggtaaat
    19021 ccggcgatca cgccgcagga gggtctgcgc gcctacctcg gcccgcagag gaatctttac
    19081 tcgggcgagg agtatgtgct caccgaggaa caccttgggc agctcgccga attacaagtg
    19141 cggaagccgg cgcggctgga ccgctatctg ttgatgcaca ccaccggaga tgaattgctc
    19201 gactggcgca tcgcggtcga ccattaccgc ggctgccggc aggtcgtcgt gcagggcagc
    19261 gatcacgggt tcaaggaatt tggcgactac ctcgatatcg ttctcgaatt cgccaaatga
    19321 cggggcacgc ttcccgtata atcgcgcgct cgcttttttt cccgtcaacc ccactcttca
    19381 cgcctcagca gcgcatgaac gttttcttcg aagaggacgg cggcttcaag gccggcaccg
    19441 tactcgccga caacaacagc tcgctgcagg tcgaaaccct gcacggcaag cgcgccaagg
    19501 taaaggccgc ggcggtgctg atccgctt
//
LOCUS       DASQOP010000249        12237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1735243, whole genome shotgun sequence.
ACCESSION   DASQOP010000249 DASQOP010000000
VERSION     DASQOP010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12237
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1735243"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VE030_09960"
     CDS             complement(<1..309)
                     /locus_tag="VE030_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase"
                     /protein_id="HZF23777.1"
                     /translation="MYQLLKQIAITGIKTEAPPQPDETLRALAQRLQERVLATFEGAL
                     TIRHVDAGSCNGCELEINSLNNPYYNLEGLGFRFVASPRHADMLLVTGPVSRHMEVAF
                     K"
     gene            complement(476..2020)
                     /locus_tag="VE030_09965"
     CDS             complement(476..2020)
                     /locus_tag="VE030_09965"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HZF23778.1"
                     /translation="MTQFDFPTYSLPSAVPAWHGTVSARQWRETAQGVLDQGGRLIAM
                     WGDDQRSRGQGFAVHAAFTMRAGLLWITLPVQSVFPGLGDLFPAAERMQRTVFDMLGV
                     AEEGARDTRPWLRHAAWPADVFPLRKDFATLHPSPITHHGEADDYPFVRVEGEGVHEI
                     PVGPVHAGIIEPGHFRFSVLGERVLRLEERLGYTHKGIEKRFEQMPLQEGVRLASRVS
                     GDSTVAYAWAYSMAAEAAAEVTVPARAAWLRALCLERERVANHLGDLGFIGNDAGLAF
                     GLAQFSRLKEDWLRTNMATFGHRYLMDAIIPGGMVRDLDRVHWSRMALEIQTVEQDVA
                     RLKAIYEDHSGLQDRFINCGKVAPELAARMGLTGLAGRASGQSWDLRVQFAAAPYDEL
                     DVRMATHRNGDVAARVIVRFEEILESLRLLRTILDKLPDGPHCVALPAVPEGRRGAGW
                     VEGWRGEVLIALETGAQGRIARCHPHDPSWQNWPVLEHAVIGNIVPDFPLINKSFNLS
                     YSGHDL"
     gene            complement(2031..3479)
                     /locus_tag="VE030_09970"
     CDS             complement(2031..3479)
                     /locus_tag="VE030_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HZF23779.1"
                     /translation="MELLLVLGIPLAGAALLAAVGHRGRAPEVNVLMSLLTFAAAASL
                     TTRVIADGPLLALNELFFVDAFNVFLVTLTAFVGFTTSLFSRPYMRIEAERGRLSPKR
                     LRLYHSMYQMFSFTMLLALLTNNIGILWVALEGATLATVLLVSLYRTPASLEAAWKYF
                     ILCGVGIAQALFGTILLYLAAERVLGAGGTALLWTHLNEVKGSLEPTVLSIAFVFLLV
                     GFGTKVGLVPLHNWLPDAHAEGPTPISAILSGLLLNVALYAIVRNKVLVDGALGGDLA
                     GNLMMGFGLLSVVVSAFFLSRQRDIKRLFAYSSIEHMGLATFAFGMGGTVATFAALLH
                     MTVHSLTKSAIFFAVGHAAQKVGTQVIDDIRGLINVSPAVGWGLMIGGLAILGIPPFG
                     VFASEFLILTTAMHDHPWATPILLVSLAVAFASIFSKVQAMVFGESTGQRLPHRPAAL
                     PVFAHLAIVLLLGLYVPPYLAQWYRQAAQMIG"
     gene            complement(3480..4142)
                     /locus_tag="VE030_09975"
     CDS             complement(3480..4142)
                     /locus_tag="VE030_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007740973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase"
                     /protein_id="HZF23780.1"
                     /translation="MNLPLSAQLINLLAALMLLLAFGMLAQRRILTLIHLFAMQGAVL
                     TANTFVVAFSSAQTHLYYSAVLTLVLKVIVLPWILHRLIDRLNIRWDVETLINIPTIM
                     LVGIVLVILSFNLALPISELAGTVNRSTLGIAMAAVLLAMLMMITRRKAVPQVIGFLA
                     MENGLFFAATSATYGMPMIVELGIALDVLVGTFIFGIFFFQIRETFDSLDIHHLEKLK
                     EK"
     gene            complement(4318..5262)
                     /locus_tag="VE030_09980"
     CDS             complement(4318..5262)
                     /locus_tag="VE030_09980"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HZF23781.1"
                     /translation="MTQFLASQLFQSLLAVLLAPLMLGWVNQCRAWLQNRTGPGMLQP
                     YRVLRKLFVKDAVMAHNASALFRLTPYVVFGCMVLAASIVPVLATDLPFSPAADVIAL
                     VGLFALARVFMALAAMDIGTAFGSMGARREMLIACLAEPALLMVFFTPSLISGSTALT
                     NIVETLAQSSLAIYPSMAFAAIAFVMVTLAENARVPIDNPATHLELTMIHEAMILEYS
                     ARHLALIEWANAIKLTVYSAIGVALFLPWGIARAGEWPMMPLAILAFAAKMAAAGAGL
                     ALLETVSAKMRIFRAPEFLGMAFMLAVLGMLTHFLLER"
     gene            complement(5262..7268)
                     /gene="hyfB"
                     /locus_tag="VE030_09985"
     CDS             complement(5262..7268)
                     /gene="hyfB"
                     /locus_tag="VE030_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HZF23782.1"
                     /translation="MSFYAPLFAAIGVMLFWFALGLAGLARGGDERFVFRFLFPLGSI
                     GGAVLAAAGLAGLSQAPAALVLPLGLPDLPFHLRLDPLSSFFLALLGLAAAGISMFSA
                     AYFRKTEVIRPGLFGLQYHLFLASMLAVCLADDAYFFMVAWETMALSSYFLVTTDHKV
                     PEIRRAGFLYLLIAHLGAISILLCFGVMQNTAGEYTFELMRGARLSDFWATAVFLLAL
                     AGFGAKAGFVPLHIWLPEAHPAAPSPVSALMSGVMLKTAIYGLLRVTFDLLDQQIWWW
                     GVVALAVGLITALLGVMFAAVQTDMKRLLAYSSIENIGVVLTAIGLAIIFHAYAMNVL
                     AALALIAALYHCINHAAFKSLLFLVTGSVMHATSERNLGHLGGLIHRMPWVAWFGLIG
                     TLAIAGLPPLNGFVSEWLLLQAFLLTPGLPNSYLNMLVPVATAALVLASALAAYVMVK
                     FYGVIFLGQPREEKLKQAHDASNWERAAFVWLAASCVLLGLAPVAVIGQLDNVSALLV
                     DATLSGNIGRSGWLFITPASPERASYSAPLFLMGIVGTVAASFLLVRWIYHGRVRRAA
                     AWDCGYPQQTARMQDTAEGFGQPIKQIFEPFFRIERHVPKPTDERPYYWSKTEDYLWY
                     WLYLPVAKAAEWGSRLVGVLHHGRIHLYLIYSFVTLLALLFLIR"
     gene            complement(7344..9386)
                     /gene="recG"
                     /locus_tag="VE030_09990"
                     /pseudo
     CDS             complement(7344..9386)
                     /gene="recG"
                     /locus_tag="VE030_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877813.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
     gene            9832..11718
                     /locus_tag="VE030_09995"
     CDS             9832..11718
                     /locus_tag="VE030_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HZF23783.1"
                     /translation="MRSASANDRKPDVFTLAVAAIGVVFGDIGTSPLYTMKEAFSGSH
                     PVAANYDNVLGVLSLVFWSLVIVVTLKYVVFMMRADNRGEGGIMALLALVLRATASGT
                     SGRRLLTTLGLFGAALFYGDGIITPAISVLSAVEGLEVATPALQPFVIPITIVILIGL
                     FVFQQKGTEMVGALFGPITAVWFAVLALLGIINIMEYPGVLVAVSPGYAVRFFAENAW
                     GGFLVLGAVVLAITGTEALYADMGHFGKKPIRLAWGAYVMPALMLNYFGQGALILHSP
                     AALAHPFYHMAPEWALFPMVILATMATVIASQAVISGTYSLTRQAIQLGYCPRLEVTH
                     TSESEIGQIYLPWINWVLLAAVIGLVLGFGSSSNLAGAYGIAVTGTMAIDTILAFVLV
                     RKLWKWPLWIALPLLLIFLTIDLSFFSANAIKLFHGGWFPIVVAILLFAVLSTWKRGR
                     ELLMERLAPGAIPIEPFIQSVTAHPPTRVPGTSVFLTAAAEGVPHALLHNLNHNKVLH
                     ERVVLLTVHTRDIPHVPDEERIEIVPLGGDFYRVIANYGFKDEPDVPRALELAAAKGL
                     KFQMMETSFFLSRQTLIPKVGPGMALWREKLFALMSRNAASATTFFRIPANRVVELGT
                     RIEL"
     gene            11739..12071
                     /locus_tag="VE030_10000"
     CDS             11739..12071
                     /locus_tag="VE030_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23784.1"
                     /translation="MDPAAVVRNLEALLSQGKDSALLRFSLGAEYLKLKETWVAVYQL
                     RRALEMDPNYSAAWKLLGKALTDAGVLRDALDAYRRGIEVAERRGDKQAAREMAVFAQ
                     RLEKQLQT"
BASE COUNT         2436 a         3911 c         3781 g         2109 t
ORIGIN      
        1 cttgaacgcc acctccatgt gccgcgacac cggtccggtc accagcagca tatcggcatg
       61 gcgcggactc gcgacgaacc tgaagcccag cccctcaagg ttgtagtacg gattgttgag
      121 cgagttgatc tcgagctcgc agccgttgca cgagcccgcg tccacgtgac gaatggtgag
      181 cgcaccctcg aacgtcgcca gcacccgttc ctgcagccgt tgcgccagcg cgcgcagcgt
      241 ctcatcgggc tgcggcggtg cctcggtctt gatgccggtg atcgcaatct gtttcagcag
      301 ttgatacatg cggggacctg cctaaccaca gagacacaga gaaacagaga accccgaaca
      361 atgaaaacgc gagtcatctg ttacgagttt gctggagatt gtggtcgccg accttactgc
      421 tgtatatcgt ttttgcattt ctccgtgcct ctgtgtctct gtggtgaata cggccttaca
      481 aatcatgacc gctgtaggac agattgaacg atttgttgat gagcgggaaa tcgggaacga
      541 tattgccgat gaccgcgtgt tcgagcaccg gccaattctg ccacgacggg tcgtgcggat
      601 ggcagcgcgc gatccggcct tgtgcgccgg tttcgagcgc gatcagcacc tcgccgcgcc
      661 agccctcgac ccagcccgcg ccgcgccggc cttccggcac cgcgggcaat gccacgcagt
      721 gcggtccgtc cggcagtttg tcgagaatcg tgcgcagcag ccgtagcgat tccagaattt
      781 cttcgaagcg cacgatcacg cgcgcggcca cgtcgccgtt gcggtgagtg gccatgcgca
      841 cgtcgagttc atcgtagggc gccgccgcaa actgcacccg cagatcccag gactgtccgc
      901 tggcacggcc cgccagtccg gtcagtccca tccttgcggc cagttcaggc gccactttcc
      961 cgcagttgat gaagcggtcc tgcagtccgg agtggtcttc gtagattgcc ttgagtctgg
     1021 ccacgtcctg ttccacggtc tggatttcca gcgccatgcg cgaccagtgc acgcggtcga
     1081 gatcgcgaac catgcctccc ggaatgatcg cgtccatcag atagcggtgg ccgaatgtcg
     1141 ccatgttcgt gcgcagccag tcctccttga gcctggagaa ctgtgccagt ccgaacgcca
     1201 gtccggcgtc gttgccgatg aagccaagat cgcccagatg attggcgacg cgctcgcgct
     1261 ccagacagag agcgcgcaac caggcggcgc gcgcaggaac cgtcacctcc gccgcggcct
     1321 cggccgccat cgaatacgcc caggcgtaag ccaccgtcga atcgccgctg acgcgtgagg
     1381 ccaagcggac accttcctgc aacggcatct gctcgaatcg tttctcgatg cctttgtgcg
     1441 tgtagccgag ccgctcctcc agccgcagca ccctttcacc caggaccgaa aagcggaagt
     1501 gtcccggctc gatgatgccg gcgtgcaccg gcccgaccgg aatctcgtgc acgccctccc
     1561 cttccacgcg gacgaagggg taatcgtccg cttccccgtg atgggtgatg ggtgatgggt
     1621 gaagggtggc gaagtccttg cggagcggga aaacgtccgc gggccaggca gcgtggcgca
     1681 gccagggacg cgtgtcgcgc gcaccttctt cggcgacgcc gagcatgtca aacacggtac
     1741 gctgcatccg ttcggcggcg ggaaacaggt cgcccagacc cggaaacacg gactgtaccg
     1801 gcagcgtgat ccacagcaga ccggcacgca tggtgaaggc ggcatgcacc gcgaaaccct
     1861 gtccgcggct gcgctggtcg tcaccccaca tcgcgatcaa ccggccgccc tggtcgagaa
     1921 cgccctgcgc cgtttcgcgc cactgccgcg cgctcaccgt gccatgccag gccggcaccg
     1981 cggacggcaa agagtaagtg ggaaagtcga actgggtcat ggcgccggtc tcaaccgatc
     2041 atctgcgccg cctgccggta ccactgggca agatacggcg ggacgtagag tccaagcagc
     2101 aacacgatcg caagatgcgc gaacaccggc aacgcggccg gacggtgtgg caggcgctgc
     2161 ccggtggatt cgccgaatac catcgcctgc accttgctga aaatcgaggc aaaggccacg
     2221 gccagcgaaa ccagcaggat cggcgtcgcc cagggatggt catgcatggc tgtagtgagg
     2281 atcaggaact cgctggcaaa cacgccgaac gggggaatac cgagtatcgc cagccctccg
     2341 atcatcagtc cccagccgac cgcggggctg acgttgatca gaccgcggat gtcgtcgatc
     2401 acctgggtgc cgaccttctg cgctgcatgg ccgaccgcga agaaaatcgc cgacttcgtc
     2461 aaagaatgca ccgtcatgtg cagcagcgcg gcaaacgttg ccaccgtccc ccccatgccg
     2521 aaagcgaaag tggccagccc catgtgctcg atcgacgagt aggcaaacag gcgtttgatg
     2581 tcgcgctgcc tggaaaggaa aaatgcggat acgaccacgg acagcaagcc gaatcccatc
     2641 atcaggttgc cggcaaggtc gccgccgagc gcaccgtcca ccagcacctt gttacgcacg
     2701 atcgcataca gcgcgacgtt caaaagcaaa ccggacagaa tcgcggagat cggagtcgga
     2761 ccttctgcgt gggcatccgg cagccagtta tgcaagggta ccagtccgac cttggtgcca
     2821 aagccgacca gcaggaacac gaacgcaatc gacagcacgg taggctcgag gctgcccttg
     2881 acctcgttca ggtgtgtcca caacagcgcc gtgccgcccg cgccgagcac gcgctccgcg
     2941 gcaaggtaaa gcaggatggt gccgaacaag gcctgcgcaa tgccgacgcc gcacaggatg
     3001 aagtacttcc aggctgcttc caggctcgcc ggcgtccggt acaggctcac cagcagcacg
     3061 gtcgcgagtg ttgcgccttc cagcgccacc cacaggatgc caatgttgtt ggtgagcagc
     3121 gccagcagca tggtgaagct gaacatctgg tacatgctgt gatagaggcg cagccgcttg
     3181 ggcgagagcc tgccgcgttc ggcttcgatg cgcatgtaag gccggctgaa caacgacgtg
     3241 gtgaatccca cgaatgcggt cagcgtcacc aggaaaacgt tgaaagcgtc gacaaagaac
     3301 agctcgttga gcgcaagcag cggtccgtcc gcgattaccc tggtggtgag cgatgccgcc
     3361 gccgcgaagg tgagcaggct catcagcacg ttcacctccg gcgcgcgccc gcgatggccg
     3421 acggccgcca acaacgccgc gccggccagg ggaatgccga gaaccagcag caactccatt
     3481 tatttttcct tcagcttttc cagatgatgg atgtcgagac tgtcgaaggt ctcgcggatc
     3541 tgaaagaaga aaatgccgaa gatgaaggtg ccgaccagca catcgagggc gatccccagc
     3601 tcgacgatca tcggcattcc gtaggttgcg ctggtagcgg cgaagaacag cccgttctcc
     3661 atcgccagga aaccgatgac ctgcggcacg gccttgcgcc gcgtgatcat catcagcatc
     3721 gccaacagca ccgctgccat cgcgatgccg agtgtcgagc ggttgacggt gccggcgagc
     3781 tcggagatcg gcaacgcaag gttgaaggaa aggatcacca gcacgatgcc caccagcatg
     3841 atggtcggga tgttgatcag cgtctccacg tcccagcgta tgttcaggcg gtcgatcagc
     3901 cggtgcagga tccagggcag cacgatgacc ttgagaacga gcgtcagcac cgccgagtaa
     3961 tacagatgcg tctgcgccga cgagaacgca acgacgaacg tgttggcggt caacaccgcg
     4021 ccctgcatcg cgaacaaatg gattagcgtc aggatgcggc gctgcgccag catgccgaat
     4081 gcgagcagca gcatcagcgc cgccagcagg ttgatcagtt gtgcggacag cggcagattc
     4141 atcttagttc aggatctttg accgcaaagg gcgcgaagga cgcaaggtaa atcttcatga
     4201 accgttgacc acgcggttga tactgtcctt gagcgccgcc tcaacattgc tctttcccat
     4261 ttttcctctt ccatgtcttt ccttcgcgtc cttcgcggtt caagactttt tcatgtttta
     4321 gcgctccagc aggaagtggg tcagcatgcc cagcaccgcg agcatgaagg ccatgccgag
     4381 aaattccgga gcgcggaaga tgcgcatttt cgccgacacg gtctcgagca gcgccaggcc
     4441 cgctccggcc gcagccatct tggcggcgaa tgcgagtatc gccagcggca tcatcggcca
     4501 ctcgcccgcg cgggcgatgc cccagggcag gaacagggcg acaccgattg cggaatagac
     4561 cgtcagcttg atcgcgttcg cccactcgat cagcgccagg tggcgcgccg agtactcgag
     4621 gatcatcgcc tcgtggatca tggtgagttc cagatgcgtg gccggattgt cgatcgggac
     4681 gcgggcgttc tccgccagcg tcaccatcac gaacgcgatg gcagcgaaag ccatgctcgg
     4741 atagatcgcc aggctgctct gtgccagcgt ctcgacgatg ttcgtcaggg cggtcgaccc
     4801 gctgatgagc gacggagtga agaacaccat cagcagcgcc ggctcggcga gacaagcaat
     4861 cagcatctcc cggcgtgcac ccatcgaacc gaacgcggtg ccgatgtcca tcgccgccag
     4921 cgccatgaac acccgcgcca aggcgaacag accgaccagc gcaatcacgt cggccgccgg
     4981 cgagaacggc aggtcggtgg ccagcaccgg cacgatcgaa gccgccagca ccatgcagcc
     5041 gaacacgacg taaggggtca gacgaaacag cgccgaagcg ttgtgggcca tcactgcatc
     5101 cttcacgaac agttttctga gcacccgata gggctgcaac atcccgggcc cggtacggtt
     5161 ctgcaaccag gcgcggcact gattgaccca gcccagcatc agcggcgcca gcaacaccgc
     5221 cagcagggac tggaacaact gggaggcgag gaactgggtc attatcggat caggaacaac
     5281 agcgccagca gtgtcacgaa gctgtagatc aggtagagat gaatacggcc gtgatgcagc
     5341 accccgacca gccgggaacc ccactcggcc gcttttgcca ccggcaggta caaccagtac
     5401 caaagatagt cctcggtttt cgaccagtaa tacggccgct cgtcggtcgg cttcggcacg
     5461 tgtctttcga tgcggaagaa cggctcgaag atctgtttga tcggctggcc gaatccttcc
     5521 gcggtgtcct gcatgcgcgc ggtctgctgc gggtagccgc agtcccaggc ggcggcgcgg
     5581 cgcaccctgc cgtggtaaat ccagcgcacc agcaggaaag aagctgccac ggtgcccacg
     5641 atgcccatca ggaaaagcgg cgcactgtaa ctcgctctct cgggggaggc gggcgtgata
     5701 aaaagccagc cggagcggcc gatgttgccc gacaaagtag cgtccaccag aagggcactg
     5761 acgttatcga gctgaccgat caccgccacc ggtgccagcc cgagcagcac acacgatgcg
     5821 gccagccaga cgaacgcggc gcgctcccaa ttggacgcat cgtgcgcctg cttgagcttc
     5881 tcctcgcgtg gctgaccgag aaatatcacg ccgtagaact tcaccatcac gtaggcagcc
     5941 agagcggagg ccagcaccag cgctgcggtc gccaccggca ccagcatgtt gagataggaa
     6001 ttcggcagcc cgggtgtcag caggaaagcc tgcagcagca gccattcgga cacaaatccg
     6061 ttcagcggcg gcagcccggc gatcgccagc gtgccgatca ggccgaacca ggccacccag
     6121 ggcatgcgat ggatcaatcc gccgagatgg ccgaggttgc gttcgctggt cgcgtgcatc
     6181 accgagccgg tgaccaggaa cagcaggctc ttgaacgcag cgtggttgat gcagtgatac
     6241 agcgccgcaa tcagcgccag cgccgcgagc acattcattg cgtaggcgtg gaagataatc
     6301 gccaacccga tcgcggtgag cacgacgccg atgttctcga tcgaggagta ggcaaggagc
     6361 cgtttcatgt cggtctgcac cgcggcaaac atgacgccca acagcgcggt aatgagtccg
     6421 accgcaagcg ccaccacacc ccaccaccag atttgctggt cgagcagatc gaaggtcacg
     6481 cgcagcaatc catagatggc ggtcttgagc atcacgccgc tcatcagcgc cgacaccggc
     6541 gagggtgccg ccgggtgtgc ctccggcagc cagatatgca gcggcacaaa acccgctttc
     6601 gcgccgaagc cggcgagcgc cagcaggaac accgcggttg cccagaaatc ggaaagcctg
     6661 gcgccgcgca tcagttcgaa ggtgtattcg cccgcggtgt tctgcatgac gccgaagcac
     6721 agcaggatcg aaatcgcgcc gagatgggca atcagcaggt aaaggaaacc ggcgcggcgg
     6781 atctccggga ccttgtggtc agtggtaacc aggaagtagg acgacagcgc catcgtttcc
     6841 caagccacca tgaaaaaata ggcgtcatcg gcaaggcaca ccgccagcat gctggcgaga
     6901 aacaagtgat attgcagacc gaacaatccc ggcctgatga cctcggtctt gcggaagtaa
     6961 gcggcggaga acatcgaaat gcccgccgcg gccaggccaa gcagcgcgag gaagaaactc
     7021 gacagcggat cgagtcgcag gtgaaacgga agatccggca gccccagcgg cagcaccagc
     7081 gcagcgggcg cctgggacag cccggcaagg ccggcggcgg caagtactgc gccgccgatg
     7141 ctgccgagcg gaaacaggaa gcggaacacg aaacgctcgt cgccacctcg cgcgagtccg
     7201 gcgagaccga gggcgaacca gaacagcatg accccgatgg ctgcgaacag cggggcgtaa
     7261 aaactcattt cgatcggcaa tgcttcaata tgtcgcggat gatagcaccc ggctcccggc
     7321 tctctcaaac cagccgggat ctctcagacc ttcagcagct cctgcctgcc gcccagccag
     7381 cgctcgagat ggcgctcggc gctcgcggca tgatcacgca gcaattgcgg cgcgacattg
     7441 cgcgccgctt ccagaagatc gatatccttt tccaggtcgg cgaagcgcag catcggcacg
     7501 ccgctctggc gcgcgcccag caactccccc gggccgcgca ggcgcagatc gtggcgtgcg
     7561 atctcgaagc cgtcggcatt ttcgtagatg atgcgcaggc gctcgcgcgc attgtccgag
     7621 agcggattct ggtacagcag gatgcagacg ctggcctctg caccgcggcc cacccggccg
     7681 cgcaactgat ggagctgcga caagcccatg cgctccgcat gctcgatcac catgagcgac
     7741 gcattgggca catccacgcc gacctcgatc accgtggtag cgaccagcaa ctgaatctgg
     7801 ttggcctgga aggccaccat caccgccgct ttctcctggt tcggcagccg cccgtgcacc
     7861 agtccaactt tcaattccgg gaaagtctgc cgcaggactt cataagtctc gatcgcggtc
     7921 tgcagctgca gcgtttcgga atcctcgatc agcggacata cccagtaggc ctgctttccc
     7981 tccaggcagg cgtcgcgcac ccgacggagc acctcgtccc ggcgcgattc ggacaacagc
     8041 ttggtcacga ccggcgtccg gcccggcggc atctcgtcga tcaccgagac atcgaggtcg
     8101 gcgtagaaac tcatcgccag cgtgcgcggg atcggcgtgg cgctcatcat cagctggtgc
     8161 ggctgcaacg cctgatcggc gcggctgccc ttcatgcgca gcgccaggcg ctggtgcacg
     8221 ccgaaacgat gctgctcgtc gacgatggcc agccccagtt ttccgaatac gacctcttcc
     8281 tggaacaacg catgggtccc aaccgcgagc tgggctttgc ctccgtcgat cgccgcaagc
     8341 accttgtcgc gttccttctt tttcaggctg ccggtgagcc acgcggtttc gattcccaac
     8401 ggctccagcc acagggagaa tttccgatag tgctgctcgg caaggatctc ggtgggagcc
     8461 atcaccgtcg cctgataacc gctctcgatt gcctgcaacg ccgcgagcgc ggctacgacg
     8521 gttttcccgc tgccgacatc accttggagc aaacgctgca tcgggtgcgg gcgcgcgaga
     8581 tcactgcgga tttcgttgag cgtgcgcgcc tgtgcgcggg taagccggaa cggcaacgac
     8641 gccagcaggc gcttcgacaa gcccgaatgc tgcaacaggg ggtgcgcccg cacgcggttg
     8701 cgccgatcgc ggtgcaatcg catcgacaat tgctgcgcca gcaattcgtc gaatttcacc
     8761 ctgcgccagg ccggatgggc gtgctcgacc agcgcctgtg tatcgacgtc gggtgtcgga
     8821 ttgtgcagca agtggaccgc ctccgcgaac ggcgggagct tgagctttct ccgcaccggt
     8881 tccggcagcg tgtccttgag gtcggcgcgc tcgagcgcac gcccggcaag tttgcgcagc
     8941 gtcgcctgcc ccaggcccgc agtggtcgga tagaccgggg tcagcgcccg cggcaacggc
     9001 gcttcaccgc gcagcacgcg gaagcgggga tgcaccatct ccgcaccgaa gaagccctgg
     9061 cggatttctc ccatcacgcg tacgcgcgca ccgggagtga gcgccttctg ctggctggga
     9121 tagaaattga agaaccgcat catcagcctg ccgctgccgt catcgacctc ggaaacgagt
     9181 tgccggcctg gacggaattt gacttcggtc tttaccaccg tgccctcgac ctgcaccgtg
     9241 tccccggaaa gcgcgtcgtg gatcgaaacg atgcgggtct cgtcctcgta gcgcagaggc
     9301 aggtgcaaca ccagatcggg gagctggaag atggcgagcc gcgccagcct gtcgcgcacc
     9361 gcaggggtgg caaatgaaag agaggggtga ggagtgaggg gtgaggagtg aggcgtaagg
     9421 ggtgaggcgt gaggcgtgag gcgcgagacg gaaaacccgg gcgtttcaga aaaagcggtc
     9481 ttttctggca ggcgcgaacc cggcggcgtc tgacgagcgg attgcttgtc tttctttggc
     9541 attgcccgtt tcacgcctca ctcctcaccc ctcacgcctc acgccttcga actgattgcc
     9601 tgtttggtca cggcccgctc cggggtatag aatgtcgcgc cacaacaagg ccccgcctct
     9661 tcgcggccag ccttgaaaat tgttaggcga tgataaccga ttgcccccag cctcccggcc
     9721 cccggcggac gaaaaaaacg aaaccatgag tcgcaagcag gcattgccgg cgaaccatcg
     9781 caagctctcg cgtccctgtt tcctgcactg atccttccga tcagaacctt gatgagatcc
     9841 gcttccgcaa acgacaggaa acccgacgta tttactctcg ctgtcgcggc catcggggtg
     9901 gtctttggcg acatcggcac cagccccttg tacacaatga aggaggcatt cagcggctcc
     9961 caccccgtgg ccgccaacta tgacaacgtg ctcggcgtgt tgtcgctcgt gttctggtcg
    10021 ctcgtcatcg tggtcacgct caaatatgtg gtgttcatga tgcgcgccga caaccgcggc
    10081 gaaggtggca tcatggccct gctggccctg gttctgcgtg ccaccgccag cggtaccagc
    10141 ggccgccggc tgctgacgac gctcgggctt ttcggtgccg cgctgttcta cggggatggc
    10201 atcatcacac cggcaatctc ggtgctgtcg gcggtggaag gcctggaagt tgccacgccc
    10261 gccctgcaac ctttcgtcat cccgatcacg atcgtgatat tgatcgggct gtttgtgttt
    10321 cagcagaaag gtaccgagat ggtcggagcg ttgttcggtc cgatcacggc cgtctggttc
    10381 gcggtgctgg cgctcctcgg catcatcaac atcatggagt acccgggtgt gctcgttgcg
    10441 gtttcgcccg gttacgctgt acggttcttt gccgagaacg cctggggcgg cttcctggta
    10501 ctcggcgcgg tggtcctagc gatcaccggc acggaagcgt tgtatgcgga catgggtcat
    10561 ttcggcaaga agccgatccg gctcgcctgg ggtgcttacg tcatgccggc gctgatgctc
    10621 aattatttcg gtcagggcgc attgatcctt catagcccgg ccgccctcgc gcacccgttt
    10681 taccacatgg ctccggagtg ggcgctcttc ccgatggtga tactggcaac catggcaacc
    10741 gtgatcgcgt cgcaggcggt catatcgggg acgtactcgc tgacacgcca ggcaatccag
    10801 ctcggctact gcccgcgcct ggaagtgact cacacgtcgg aaagcgaaat cggccagatt
    10861 tatttgccct ggatcaactg ggtcctgctg gcggcggtga tcgggttggt gctcggcttc
    10921 ggctcctcga gcaatctcgc gggcgcctac ggcatagcgg ttaccggcac aatggccatc
    10981 gacaccatcc tcgccttcgt cctggtacga aaattgtgga agtggccgct atggattgca
    11041 ctgccgctcc tgttgatctt tcttaccatc gacctgtcat ttttcagcgc caacgcaatc
    11101 aaactctttc atggcggctg gttcccgatc gtcgtcgcaa tcctgctgtt cgcagtgctg
    11161 tccacctgga agcgcgggcg cgagctgttg atggagcggc tggccccggg ggcgatcccg
    11221 atcgaaccgt tcattcagag cgtgaccgca catccgccga cccgtgtgcc cggcacctcg
    11281 gtgttcctga ccgctgcggc cgaaggcgtg ccgcatgcgc tgctgcacaa cctcaaccac
    11341 aacaaggtgc tgcacgaacg cgtcgtgctg ctcaccgtgc acacgcgcga catcccccac
    11401 gtccccgacg aagagcgcat cgagatcgtg ccgcttggcg gcgactttta ccgggttatc
    11461 gcgaactacg ggttcaagga cgaaccggac gtgccgcgcg cgctcgaact ggcggccgcg
    11521 aaggggctga agttccagat gatggagacc tcgttcttcc tgagccggca gacgctgatt
    11581 ccgaaagtcg ggcccggcat ggcgctgtgg cgcgagaagc tttttgccct gatgtcccgc
    11641 aatgccgcaa gcgccacgac attcttcagg attcccgcca atcgcgtggt tgagctcggc
    11701 acccgcatcg aactctagcc gccaacgaca ggtttcgcat ggatcccgcg gcggtggtcc
    11761 gcaatctcga ggcccttttg tcgcagggaa aagactcggc gctgctgcgc ttcagcctgg
    11821 gagccgagta tctcaagctg aaggaaacgt gggtcgccgt ataccagttg cggcgcgcgc
    11881 tcgagatgga ccccaattat tccgccgcat ggaagctgct cggcaaggcg ctgaccgatg
    11941 ctggtgtcct gcgcgatgct ctcgacgcct atcgccgcgg aatcgaagtc gcggagcggc
    12001 gcggcgataa acaggcggca agggagatgg ccgtgttcgc gcagcgactc gagaagcagc
    12061 tgcaaacctg agtgcgtcga cgtgacagtc cggtcgggct ggccgatctc cggcgtggat
    12121 ttcaccagcc gcccccgcag gcaaaaaccg atcgtcatcg cgaccggcag aatccacggt
    12181 cgcgcattcg gactcgaggg catcgaagag tattgcgatt gggagaagta cgaatcc
//
LOCUS       DASQOP010000250         6846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_724251, whole genome shotgun sequence.
ACCESSION   DASQOP010000250 DASQOP010000000
VERSION     DASQOP010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6846
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724251"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2745)
                     /gene="smc"
                     /locus_tag="VE030_10005"
     CDS             complement(<1..2745)
                     /gene="smc"
                     /locus_tag="VE030_10005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HZF23785.1"
                     /translation="MRLTHIKLAGFKSFVDPTHIPVPGQLVGVVGPNGCGKSNVIDAV
                     RWVLGESSAKQLRGENMQDVIFNGSGERRPVGRASVELIFDNSLGKAAGPWSQYGEIS
                     VKRVLERDGESSYFVNNLHVRRRDVADIFLGTGLGGRGYAIIEQGMISRIIEARPEDL
                     RSFLEEAAGVSKYRERRRETELRLDDTRENLLRVDDIRRELGAQLEHLESQAERAGRY
                     RELEAELKRTQHTLWLLRKRDAAAQRARIGRDIEATGVELEAETASLRDAEKRVEELR
                     AGHYSASDAVHAAQGALYEVYAEVARIEQQIQYLHDSRNRAEHQLESVRAQEAEQRSR
                     QASQEAARSASEESLAKARRQLAALADGLQAARAALPAAEEAYRNAQAQLAEAQHRLT
                     EAEQQLKLEEARAGHGEKLLLQLETRRARLEEERDGLEVPNLSELVRLETEIGELAQR
                     LYEQRDALEKCDADLPTLEATMHGADQDAEQTQQHLNQLDARITALQSLQERVSRAGE
                     IEPWLERHELRSNPHFWQGIRIREGWENALEAVLGERLNSVGLIDLAQVHAWLAETPP
                     GKVSFHRSAGAAAAVEPAVAGLTRLSDYVICTDARLAAVLGDWLARVYVLPDPARGVE
                     LARDLPAGAMLVSAQGHVFTATSVSFHAADSEVHGILSRQRDIEALFAERGSADEELG
                     RQRAAAMSRKSEFEERRTQAECLRRTVAELQEHHHQLKLDHVRLSQLAERLKERGEQI
                     VRELAEIDAEMARESVQCESASHQIDALAAGMDALRAGVESAGQAHANAESALQGSRV
                     NAQQIEREHQEAQFQETTISTKIIDIKESLDVISQSLKRLASDSEALSEEISRSDEGP
                     LQTQLQVQLDARVEREQSLKQGRDELQAAEDRLRATELERLGSEQKLAPLRERINELR
                     L"
     gene            2874..3290
                     /gene="queF"
                     /locus_tag="VE030_10010"
     CDS             2874..3290
                     /gene="queF"
                     /locus_tag="VE030_10010"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HZF23786.1"
                     /translation="MPSKPALTLATFPNPNPQRDYLIHMEIPEFTCLCPMTGQPDFAT
                     LYLDYVPDRKCVELKSLKTYIWAYRDRGAYHEAVTNRILDDLATATKPRFMRLTARFY
                     VRGGIFTTVIAEHRKKGWMPAPRIELDSIAPQSSVR"
     gene            3290..4540
                     /gene="dapC"
                     /locus_tag="VE030_10015"
     CDS             3290..4540
                     /gene="dapC"
                     /locus_tag="VE030_10015"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006498676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HZF23787.1"
                     /translation="MNPNLTRLQPYPFQKLAALLSEVKANASLRPINLSIGEPKHATP
                     QFIREALSAKLDGLSSYPATAGSETLRAAIAAWIERRYRLPRIDPVKQVLPVNGSREA
                     LFAFAQAVVNASREGPVVVAPNPFYQIYEGAALLAGAEPQFLNILPHNRFALDFGQLP
                     ESVWSRTQLVYVCSPGNPTGSVMTLEEWQTLFDLSDRHGFVIASDECYSEIYFDEGHA
                     PLGGLEAAHRLGRGQFPRLVAFGSLSKRSNVPGMRSGYVAGDADILAKFLLYRTYHGC
                     AMSPAVQAASTAAWQDEEHVRDNRRLYREKFQKAVALLAEVTDAPMPQAAFYLWLRTP
                     IADTEFAKRLFEQYNVSVVPGSFLAREARGVNPGANFVRIALVAPVEECVEAAQRLAE
                     FFSQHSQGKNRNDGFAVGHREGLR"
     gene            4500..5321
                     /gene="dapD"
                     /locus_tag="VE030_10020"
     CDS             4500..5321
                     /gene="dapD"
                     /locus_tag="VE030_10020"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HZF23788.1"
                     /translation="MTDLQSVIGKAFDNRANISPNNAEPALKDAVAEVIDGLDAGTLR
                     VAEKIGGEWVTHQWVKKAVLLSFRLQDNAVVRDGYTNYFDKVPAKFADYGAADFRAGG
                     FRVVPPAAARRGSFIARNVVLMPSFVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPVQANPTIIEDNCFIGARSEIVEGVAVGEGSVISMGVYIGSSTKIYNRQ
                     TGETLYGRIPPGSVVVSGNLPSADGKYSLYCAVIVKQVDAKTRAKTSINELLRGD"
     gene            5389..6561
                     /locus_tag="VE030_10025"
     CDS             5389..6561
                     /locus_tag="VE030_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HZF23789.1"
                     /translation="MFLSPLFKLMAEKQASDLFISASAPVSIKILGGIMPVNAQVIDA
                     EGARKIAYEVMTDRQIREFEETMEMNFSYVVQDIGRFRVNVFRQRGNVAMVIRYLKSG
                     TPGIEKLKLPTILKDLVLEKRGFVVVAGSTGSGKSTTLAAMIEHRNAQKPGHILTIED
                     PMEFVFRNDKCVINQREVGTDTRSYENALVNAMREAPDMMMIGEIRDRQTLQHALLYA
                     QTGHLCLSTLHANNSYHALNRIINFFPYEARNSLLSDLSICLRAIISQRLVKANDGSQ
                     VAAVEVMLNTKHVAELIKKGEIDQIKEAMEQSLSPGSKTFEHALFELFMAGTVSKDEA
                     LKNSDSATNLSWLINNYEQKASGKIGESPTGKVMVKPGGGYSFSEIKLNVDTDSKK"
     gene            6608..>6846
                     /locus_tag="VE030_10030"
     CDS             6608..>6846
                     /locus_tag="VE030_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014111481.1"
                     /note="dapE-encoded N-succinyl-L,L-diaminopimelic acid
                     desuccinylase (DapE), catalyzes the hydrolysis of
                     N-succinyl-L,Ldiaminopimelate L,L-SDAP to
                     L,L-diaminopimelate and succinate. It is a metalloprotease
                     containing dinuclear active sites. Its structure is
                     similar to the carboxypeptidase G2 from Pseudomonas sp.
                     strain RS-16 and the aminopeptidase from Aeromonas
                     proteolytica; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HZF23790.1"
                     /translation="MSSATFELARELISRASVTPNDAGCQEILRQRLARLGFKAEPMR
                     HGEVDNLWMRRGSRAPLVCFAGHSDVVPTGPLDQWR"
BASE COUNT         1231 a         2275 c         2071 g         1269 t
ORIGIN      
        1 gagacgcagt tcgttgatgc gttcgcgcaa cggcgccagc ttctgttccg acccgaggcg
       61 ctccagctcg gtagcacgca gcctgtcctc ggcggcctgc aattcatcgc ggccctgctt
      121 caggctttgc tcgcgctcca cacgggcatc aagttgcacc tgcagttgcg tctggagtgg
      181 gccctcgtcg ctccgggaga tctcctcgga cagcgcctcg gaatcgctgg cgaggcgctt
      241 cagactctga gaaataacat caagtgattc ttttatatca atgatcttag tcgatatagt
      301 tgtttcctga aactgggctt cctggtgctc ccgctcgatt tgctgtgcgt tcacccggct
      361 gccttgcaag gccgattccg cgtttgcgtg cgcctgtcca gcggattcga ccccggcccg
      421 caacgcatcc attccggcgg ccagtgcatc gatctgatgg ctcgccgact cgcactgcac
      481 cgactcgcgc gccatctcgg catcgatctc cgcaagctcg cgcacgatct gctcgccacg
      541 ctccttgagg cgctcggcaa gctgcgacag gcgcacgtgg tccagtttca gctggtgatg
      601 gtgctcctgc aattcggcca ccgtgcgccg caggcactcg gcctgcgtgc ggcgttcctc
      661 gaattcggat ttgcgggaca tcgcggcggc gcgttgccgt cccagttcct cgtcagcact
      721 gccgcgctcc gcgaacaacg cctcgatatc acgctggcgc gacaggatgc cgtgcacttc
      781 cgaatcggcc gcatggaaac tgacgctggt ggcggtgaat acatgccctt gcgcactgac
      841 cagcatcgcg ccggcgggca ggtcgcgggc cagttccacg cccctggcag gatccggcag
      901 cacgtagacc cgcgccagcc agtcgccaag caccgctgcc agccgcgcgt cggtgcagat
      961 cacataatcg ctgagccgtg taagccccgc cacggcaggc tcgacggcgg cggcagcccc
     1021 agcgctgcgg tggaaggaaa ccttccccgg cggcgtctcc gccagccagg catgcacctg
     1081 agcgaggtcg atgagaccga cgctgttcag gcgctcgccc aataccgctt cgagtgcgtt
     1141 ttcccagcct tcccggatcc tgattccctg ccagaagtgc gggttgctgc gcaattcgtg
     1201 gcgttccagc cagggttcga tttctcccgc ccgactcacc cgctcctgca gcgactgcag
     1261 cgcagtgatc cgggcatcga gctggttgag atgctgctgg gtctgctcgg catcctggtc
     1321 cgcgccgtgc atcgttgctt cgagcgtggg caaatcggcg tcgcactttt cgagcgcatc
     1381 gcgctgttca tagagccgct gagccagctc cccgatctcc gtttccagcc ttaccagttc
     1441 gctcaggttc ggcacttcca ggccgtcgcg ctcttcttcc aatcgcgcgc gccgcgtttc
     1501 aagctgcagc agcagcttct cgccgtggcc ggcgcgggct tcctccagtt tcagctgttg
     1561 ttcggcttcg gtgaggcggt gttgcgcctc ggcaagctgc gcctgtgcgt tccgatacgc
     1621 ctcttcagcg gccggcaagg cggctcgcgc ggcctgcagt ccatcagcaa gcgcggccag
     1681 ttgcctgcga gcctttgcca gcgactcctc gctggcgctg cgcgccgcct cttgtgacgc
     1741 ctgccggctg cgctgctcgg cttcctgcgc ccgcaccgac tcgagctggt gctcggcgcg
     1801 attgcggctg tcgtggaggt actggatctg ctgctcgatg cgggcgactt cggcgtagac
     1861 ctcgtagagc gcgccctggg cggcgtgtac cgcatccgaa gcgctgtaat ggccggcgcg
     1921 caattcctcg acgcgctttt cggcatcgcg caggctggcg gtctcggctt ccagctccac
     1981 gcccgtcgcc tcgatatcgc ggccgatgcg ggcgcgctgc gctgcggcgt cgcgtttgcg
     2041 cagcagccac aacgtgtgct gtgtacgctt cagctcagcc tccagctccc ggtatctgcc
     2101 cgccctttcg gcctgggatt caaggtgctc gagctgtgcc ccgagctccc ggcggatatc
     2161 gtccacccgc agcaggtttt cgcgcgtgtc gtcgagcctg agttcggttt cgcgccggcg
     2221 ctcgcggtat ttcgatacgc cggccgcctc ctcgaggaag ctgcgcaggt cttccggccg
     2281 ggcctcgatg atgcgcgaaa tcatcccctg ctcgatgatc gcgtagccgc ggccgcccag
     2341 gccggtaccc aggaaaatgt cggcgacgtc gcgccggcgc acgtgcaagt tgttgacgaa
     2401 ataagacgac tctccgtcgc gctcgagcac gcgcttgacc gaaatctcgc cgtactgcga
     2461 ccacggcccg gctgccttgc ccagactgtt gtcgaaaatc agctctacgc tggcgcggcc
     2521 gaccggcctg cgctcaccgg agccgttgaa gatgacatcc tgcatgttct cgccgcgaag
     2581 ctgcttggcc gaggattccc cgagcaccca gcgcaccgcg tcgatcacgt tggatttgcc
     2641 gcagccgttg ggaccgacga cgccgaccag ttggccgggg accgggatgt gggtagggtc
     2701 gacgaaggac ttgaaaccgg ccagtttgat gtgagtgagt cgcactgtcg caattctata
     2761 aaggcttata attttgaacg gatgcccata aaacaagcgc ctggggcccc taacaaggac
     2821 caggcgcgtg ttttttcagg gcgtatttta acaactgaat cgcccccaag agaatgccga
     2881 gcaagccggc gctgaccctg gcgaccttcc ccaatccgaa tccgcagcgg gattacctga
     2941 ttcatatgga gattccggaa ttcacctgcc tctgcccgat gaccggacag ccggacttcg
     3001 ccacgcttta cctcgattac gttcccgacc gcaaatgcgt ggaactcaag agcctgaaaa
     3061 cctatatctg ggcctaccgc gaccggggcg cctaccacga agccgtcacc aaccgcattc
     3121 tcgacgatct agccacggcg acgaagcctc gcttcatgcg cctgactgca cgcttctacg
     3181 tgcgtggggg catcttcacc accgtcatcg ccgaacaccg caagaagggc tggatgccgg
     3241 caccgcgcat cgaactcgac agcatcgcgc cgcagtcaag cgtacgctga tgaatccgaa
     3301 cctgaccagg ctgcaaccct accctttcca gaagcttgcc gcgctgctct cggaagtgaa
     3361 agccaacgcc tcgttgcgtc cgatcaatct gtcgatcggc gagccgaagc atgccacacc
     3421 gcagttcatt cgcgaggcct tgagcgccaa gctggatgga ttgtcgagct accccgcaac
     3481 cgcgggcagc gaaacgctgc gcgccgcaat cgcggcatgg atcgagcggc gttaccggct
     3541 gccacgtatc gacccggtaa aacaggtgct gccggtcaat ggcagccggg aagcgctgtt
     3601 cgcgttcgcg caggctgttg tgaacgcctc gcgcgaaggg ccggtcgtgg tggccccgaa
     3661 tcccttctat cagatctacg aaggcgccgc gctcctggcc ggggcagaac cgcaattcct
     3721 caatattctc ccgcacaacc gcttcgctct cgatttcggc cagttgcccg aatcggtgtg
     3781 gagccgcacc cagctggtct acgtctgctc gccgggcaat cccaccggca gcgtcatgac
     3841 cctcgaagag tggcagactc tgttcgatct ttccgatcgc cacggcttcg tgatcgcctc
     3901 cgacgagtgc tactcggaga tctatttcga cgaaggccat gcgccgctcg gcggcctgga
     3961 agccgcgcat cgcctcggcc gtggccagtt cccgcgtctg gtcgcattcg gcagcctgtc
     4021 gaaacgctcg aacgtgcccg ggatgcgctc cggctacgtc gccggcgatg cggacatcct
     4081 ggcgaagttc ctgctttacc ggacttacca cggctgcgcg atgagccctg cggtgcaggc
     4141 cgccagtacc gcggcgtggc aggacgaaga gcacgtgcgc gacaaccggc ggctataccg
     4201 ggagaaattc cagaaagcag tggccctgct tgccgaagtc accgatgccc ccatgccgca
     4261 agcggcgttc tacctttggc tgcgcacccc gatcgccgac acggaattcg ctaaacgctt
     4321 gtttgagcaa tataatgtaa gcgtcgtacc cggcagtttt ctggcccgcg aggcccgcgg
     4381 cgtcaatccg ggggcgaact tcgtgcgcat cgcgctggtg gccccggtcg aggaatgcgt
     4441 cgaagcggcg caacggctgg ccgaattttt ctctcaacac tcacaaggaa aaaacaggaa
     4501 tgacggattt gcagtcggtc atcgggaagg ccttcgataa ccgtgccaac atctcgccaa
     4561 acaacgccga acccgccctc aaggacgccg ttgccgaagt tatcgatggt ctggacgccg
     4621 gtacgctgcg ggtcgcggag aaaatcggcg gcgagtgggt cacccaccag tgggtcaaga
     4681 aagccgtcct gctgtcgttc cggctgcagg acaacgcagt ggtccgcgac ggctacacca
     4741 attacttcga caaggtgccg gccaagttcg ccgactacgg cgctgccgat tttcgcgccg
     4801 gcgggttccg cgtagtgccg ccggcggcgg cgcggcgcgg ctcgttcatc gccaggaacg
     4861 tggtgctgat gccctccttc gtgaatatcg gcgcctacgt ggatgaaggc accatggtgg
     4921 acacttgggc cacggtgggc tcatgcgcgc agatcggcaa gaacgtccac ctctccggag
     4981 gcgtggggat cggcggagtt ctggaaccgg tacaggccaa tccgaccatc atcgaggaca
     5041 attgtttcat cggcgcccgc tccgagatcg tcgagggcgt ggcggtcggc gaaggctcgg
     5101 tgatctcgat gggcgtctac atcggcagca gcacaaagat ctacaatcgt cagaccggcg
     5161 aaaccctcta cggcaggatt ccacccgggt ctgtggtcgt gtcgggcaac ctgccctcgg
     5221 ccgacggcaa atacagtctt tactgcgcgg tcatcgtcaa gcaggtagac gccaagaccc
     5281 gcgccaagac cagcatcaac gagctcctcc gcggcgacta gcaggagcct tccggttacg
     5341 cggcgcggat cggcgcaacc actactacca ggagtcatcg aggccaccat gtttctaagc
     5401 ccactattca agctgatggc ggaaaagcag gcatcggatc ttttcatctc ggccagcgcc
     5461 ccggtgtcga tcaagatcct cggcggcatc atgccggtga acgcgcaggt gatcgatgcg
     5521 gagggcgccc gcaagatcgc ctacgaagtg atgactgacc ggcagatacg cgagttcgag
     5581 gagaccatgg agatgaactt ctcctacgta gtccaggaca tcggccgttt ccgcgtcaac
     5641 gtcttccgcc agcgcggcaa cgtggcgatg gtgatccgct atctcaagag cggtacgccg
     5701 gggatcgaaa agctcaagct gcccacgatc ctcaaggacc tggtgctgga gaagcgcggg
     5761 ttcgtcgttg tggccgggtc caccggttcc ggcaagtcca ccacgctcgc cgccatgatc
     5821 gagcaccgca acgctcaaaa gcccgggcac atcctcacca tcgaggaccc gatggagttc
     5881 gttttccgca acgacaagtg cgtgatcaac cagcgcgaag tcggcaccga cacccgctcc
     5941 tacgaaaacg cgctggtgaa cgcgatgcgc gaagcgccgg acatgatgat gatcggcgag
     6001 atccgcgacc gccagacgct gcagcacgcg ctgctctacg cgcagaccgg ccacctgtgc
     6061 ctgtccacgc tgcacgccaa caacagctac cacgcgctca accgcatcat caacttcttc
     6121 ccctacgaag cgcgcaattc gctgctgtcc gacctttcca tctgcctgcg cgccatcatt
     6181 tcccagcggc tggtcaaggc gaacgacggt tcgcaggtcg ctgcggtgga ggtcatgctc
     6241 aacaccaagc acgtcgccga actgatcaag aagggcgaaa tcgaccagat caaagaagcg
     6301 atggagcaaa gcctttcgcc cgggtcgaag accttcgagc acgccctgtt cgaactgttc
     6361 atggcaggga ccgtttccaa ggacgaggcg ctgaagaact cggattccgc caccaacctg
     6421 tcctggctga tcaacaatta tgaacagaag gcctccggca agatcggcga gagcccgacc
     6481 gggaaagtga tggtcaagcc gggcggaggt tactcgttca gcgaaatcaa gctgaacgtg
     6541 gataccgaca gcaagaagtg aaccgccgcc cggttctccg gcgcccgtga tgccggactc
     6601 ggccgccatg tcgtccgcca ccttcgaact tgcgcgcgag ctgatctcgc gcgcttcggt
     6661 tacgccaaac gatgccggat gccaggagat cctgcggcag cggctggcgc ggctaggctt
     6721 caaggcggag ccgatgcgcc acggcgaagt cgacaatctc tggatgcggc gcggcagccg
     6781 cgcgccgctc gtctgcttcg cgggacacag cgacgtggtc ccgaccgggc cgctggacca
     6841 gtggcg
//
LOCUS       DASQOP010000251         2208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2941474, whole genome shotgun sequence.
ACCESSION   DASQOP010000251 DASQOP010000000
VERSION     DASQOP010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2941474"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VE030_10035"
     CDS             complement(<1..329)
                     /locus_tag="VE030_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23791.1"
                     /translation="MNKLFQENQPLINVGLQSFADSVMAAGGKAVHLDWTPPAGGNRV
                     AGWCLAQLLNHPSLEAANEKAYAAFLAAQPVLAGVGVAREHLPGMGDRMILHAGPPIA
                     WEEMCGP"
     gene            complement(347..1921)
                     /gene="fdrA"
                     /locus_tag="VE030_10040"
     CDS             complement(347..1921)
                     /gene="fdrA"
                     /locus_tag="VE030_10040"
                     /inference="COORDINATES: protein motif:HMM:NF004760.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HZF23792.1"
                     /translation="MSATLVKIVPREYRDSVSLMQLSAMLGKLPGVEQASAAMATENN
                     LVLLAEAGLRVDAKGAGASDLLIVVQGEKSALPAAMEEAMKSLTRQTEDNGAEARVAL
                     RPRSIQMALKTTPEANMALISTPGDYAAAEAMKALRLGLNVMLFSNNVSEEEELLLKR
                     FARDADLIVMGPDCGTAIINGTPLAFANVVKRGPVGVVGASGTGLQQVTVLVDRLGSG
                     ISQAIGTGGRDLHRDIGAISMLKGLKDLAADPATQVIVLISKPPAPEVAERVLAEAAQ
                     AGKPVVVNFLGTDPKQVGRKGVHAVKTLEDAARAAVAIVKGEKPRDSYAEAPSAPSGL
                     PRFANTQRYVRGLYSGGTFCYEATLLLGELLGDVYSNTPTHGTTELADIWQSRQHTLI
                     DLGDDVFTRGRPHPMIDYRLRTERIAKEAEDPETAVILLDVVLGYGSHMDPSSELVPA
                     INRARTTASAQGRVIVFVGQICGTAADPQDFARQTRALADAGMLFAESNAQAVRLAAG
                     IASTLAPSAAQRLRKS"
     gene            complement(1903..>2208)
                     /locus_tag="VE030_10045"
     CDS             complement(1903..>2208)
                     /locus_tag="VE030_10045"
                     /inference="COORDINATES: protein motif:HMM:NF022834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HZF23793.1"
                     /translation="NAISRQMLLDALQGQYAERLTEVVRCVCGVGDVAVASMRALEVG
                     HSSGADVLCGLLFGYSPTLIARPIQFARTDHFFCDCQAAHGSDRLLIRENRTCRPHW"
BASE COUNT          363 a          748 c          699 g          398 t
ORIGIN      
        1 atcggcccgc acatttcctc ccaggcgatc ggaggtccgg cgtgcaggat catgcgatcg
       61 cccatgcccg gcaagtgctc gcgcgcaaca ccgaccccgg caagcaccgg ctgcgcagcg
      121 aggaaggcgg cgtacgcttt ctcgttcgcg gcttccagcg acggatgatt cagcaactgc
      181 gccaggcacc agccggccac gcggttgccc ccggcgggcg gagtccaatc cagatgtacc
      241 gccttgcctc cggccgccat gacggaatcg gcgaacgact gcaatccgac gttgatcagc
      301 ggctggtttt cctggaacag cttgttcatt gcgttctggt cccgaactag gatttgcgca
      361 agcgctgcgc cgccgacggg gcgagcgtgg aggcgatgcc ggccgcaaga cgcacggcct
      421 gcgcattgct ttcggcgaac agcatgccgg catcagcaag cgcccgcgtc tgccgggcga
      481 aatcctgcgg atcggcggcg gtgccgcaga tctggccgac gaagacgatg actcttccct
      541 gcgcggacgc ggtcgtccgt gcgcgattta tcgccggcac caattcgctc gacgggtcca
      601 tgtggctgcc gtatcccagc accacgtcga gcaggatcac ggcggtttcc ggatcttccg
      661 cctccttggc gatgcgctcg gtgcgcaggc ggtagtcgat catcgggtgc gggcgtccgc
      721 gcgtgaagac gtcgtctccg aggtcgatca gcgtgtgctg ccggctttgc cagatgtcgg
      781 caagctcggt ggttccgtgc gtgggcgtat tggagtacac gtcgcccagg agttcgccca
      841 gcaacagcgt ggcctcataa cagaacgtgc cgccgctgta gagtccgcgg acgtaacgct
      901 gtgtattggc gaaacgcggc agcccggacg gcgcggacgg ggcttcggcg taactgtcgc
      961 gcggcttctc acccttgacg atggcgaccg ccgcgcgcgc cgcatcctcc agcgtcttca
     1021 ctgcatgcac gcccttgcgc ccgacctgct tcggatcggt gccgagaaaa ttcaccacca
     1081 ccggcttgcc ggcctgggcg gcctcggcga gcacgcgctc ggccacttcg ggagcaggcg
     1141 gtttggatat cagcacgatg acctgggtgg cggggtccgc ggcaaggtcc ttaagaccct
     1201 tgagcatcga gatcgcgccg atatcgcggt gcagatcgcg cccgccggtg ccgattgcct
     1261 gcgagatccc cgatccaagc cggtccacca gcacggtgac ctgctgcaat cccgtgcccg
     1321 aggcgccgac cacgcccacg gggccgcgct tcaccacgtt ggcgaaggca agcggcgttc
     1381 cgttgatgat cgcggtgccg cagtccgggc ccatgacgat gagatcggcg tcgcgtgcga
     1441 accgcttgag caggagttcc tcctcctcgg agacgttgtt gctgaacaac atcacgttga
     1501 gtccgaggcg cagggctttc attgcttcgg ccgccgcgta gtctcccggt gtggaaatga
     1561 gcgccatgtt ggcttcgggc gtggtcttca gcgccatctg gatgctgcgc ggtcgcagcg
     1621 ccacccgcgc ttcagcgccg ttgtcctcgg tctgccgcgt gaggctcttc atcgcctcct
     1681 ccatggcggc aggcagcgcc gacttctctc cctgcaccac gatcagcaag tcgctcgccc
     1741 ccgcgccttt cgcgtccacg cgcaaccctg cctctgcgag cagtaccagg ttgttttcgg
     1801 tggccatcgc tgcggaagcc tgctccacgc ccgggagctt ccccagcatc gcggacagtt
     1861 gcatcagcga gaccgagtcc cggtattccc tgggcacgat tttcaccagt gtggccgaca
     1921 agtcctattc tccctgatca acaggcgatc gctgccgtgc gcagcttggc aatcgcaaaa
     1981 aaaatggtct gtgcgggcaa actggatcgg cctcgcaatc agcgtgggcg aatagccgaa
     2041 cagcagcccg cataaaacat ccgcgcccga actgtgtccg acttccagcg cccgcatgct
     2101 ggcgacagcg acgtcgccga caccgcaaac gcaacgcacg acctcggtca ggcgctcagc
     2161 gtattgacct tgaagcgcgt cgagcaacat ctggcggctg atcgcgtt
//
LOCUS       DASQOP010000252         4000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2741548, whole genome shotgun sequence.
ACCESSION   DASQOP010000252 DASQOP010000000
VERSION     DASQOP010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2741548"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="VE030_10050"
     CDS             complement(<1..753)
                     /locus_tag="VE030_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012492422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HZF23794.1"
                     /translation="MKYILALDQGTTSSRAMVFDHSGSVVALAQKEFRQIFPKPGWVE
                     HDATEIWATQLHTATEALGQAGLTATDIGAIGITNQRETTVVWDRDTGEPVHHAIVWQ
                     DRRTAARCDQLKARGLAPMIKRKTGLVVDAYFSGTKLEWILRNVPGAGAKAKAGKLAF
                     GTVDSWLVWNLTGGRVHVTDPSNASRTMLFNLRTGDWDDELLKLFGVPRSVLPAVGSS
                     SEVYGETTLFAGGRAIPIAGIAGDQQAALFGQA"
     gene            complement(750..1481)
                     /locus_tag="VE030_10055"
     CDS             complement(750..1481)
                     /locus_tag="VE030_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HZF23795.1"
                     /translation="MPPFLAELLGTMLLIVLGDGVVANVVLNKTKGQNAGWIVITAGW
                     GFGVAVAVYAVGAFSGAHLNPAVTIGLASIGKFPWASVPGYIAAQMLGALLGATIVWL
                     AYLPHWAVTESQAGKLAVFCTGPAIRDPAANLLTEVIGTFVLVLGVLAILTPKNLNPL
                     HGWDAGFGPFLVGILVWAIGLSLGGPTGYAINPARDLGPRIAHALLPIAGKGNSDWGY
                     AWIPVAGPIIGGLIAAFVYKTWWPA"
     gene            complement(1538..>4000)
                     /locus_tag="VE030_10060"
     CDS             complement(1538..>4000)
                     /locus_tag="VE030_10060"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZF23796.1"
                     /translation="VALKVLRPELAAVLGGERFLREISLTAQLQHTHILTLIDSGEAD
                     GFLYYVMPYVEGESLRQRLEREGQLPLDEALSITRAIASALDFAHGRGVIHRDIKPEN
                     VMLHQGEPMVADFGIALAVSTAGRERLTETGLSLGTPAYMSPEQASAEPRLDGRSDQY
                     SLASVLYEMLAGEPPYNGPTAQAIIAKCLTEPIPHLGTVRRVTPAVEAAVTKALAKAP
                     ADRFATAGAFVAALTAGSTRAVRPRWQSAGGALAVLIIIGGAAAWFYASKSSQVGQDH
                     PNVVPFTSSPGQKFHPVFSPDGNQIAYAWRGENDDNSDIYVKLIGAGGPLRLTSSPAR
                     EYCPAWSPDGRYIAFIRDGPSSDRAAYYLIPALGGAERKIGEAYDRSQVSGRCIDWSL
                     DGKYLIVADRMAPEESRSSILLLSVEDGQRKVFVSQPDLYVADPILSPDGTAIAYAGG
                     AGFIASDIYVVPASGGGSRRLTSDGRFLAGLAWTPDGKEIVFTSNRGGLMRLWRISSS
                     GGTPALVNGVGEGASSPTISTKGDRLAYINGRDDANLWRTRGPVWKGRPPPPMKMVAS
                     SRADYEAAYAPDGKRIAFASDRSGSMEIWTCNSDGTNQAQLTSLKATDAGSPSWSPDG
                     KTIAFDARLEGHGDIFVISADGGAPRRLTSDPVDNNVPTWSRDGKWIYFSSSRTGNWQ
                     IWKVPSGGGSAVQVTKSGGFSAQESPDAKSLYVWVQGGTIWKMRLPGAEPVRVLPGVQ
                     LFVWWKITRDGIYFIDGSTIPARVRLLDLATQRVRTITSMDLGPGAPGAQYFDLSPDG
                     QWILFDRLDQVESDIMLIENFR"
BASE COUNT          755 a         1279 c         1257 g          709 t
ORIGIN      
        1 cgcctggccg aacagcgcag cctgctgatc gcccgcaatg ccggcaatcg gaatggcgcg
       61 gcccccggcg aacagcgtcg tttcgccgta cacttcgctc gacgagccga ccgcgggcaa
      121 caccgagcgc ggcacgccga acagcttcag cagctcgtcg tcccaatcgc cggttcggag
      181 attgaagagc atcgtgcgcg acgcgttgct cggatcggtc acgtgcacgc ggccgcccgt
      241 caagttccat acgagccacg agtccacggt gccgaatgcc agcttgccgg ccttggcttt
      301 ggcccccgcg cccggaacat tgcgcagaat ccattcgagt tttgtgccgg aaaaataggc
      361 atctaccacg aggccggtct tgcgcttgat catgggcgcc agcccgcggg ccttcaactg
      421 gtcgcaccgg gcggcggtgc gacgatcctg ccacacaatg gcgtgatgca ccggctcgcc
      481 ggtgtcgcga tcccagacta cggttgtctc ccgctgattc gtgatcccga tggcgccgat
      541 gtccgtggca gtcagtccgg cctggccgag cgcttccgtc gcggtgtgca actgcgtggc
      601 ccagatctcc gtcgcgtcgt gctccaccca gccgggcttg gggaaaatct gacgaaactc
      661 cttctgcgcc aacgcaacca cgctcccgct gtggtcgaac accatggcgc gcgagctcgt
      721 ggtgccctgg tcgagggcca ggatgtattt cacgccggcc accaggtctt gtacacgaag
      781 gccgcgatca ggccgccgat gatcggtccg gccaccggta tccacgcgta gccccagtcc
      841 gaattgccct tgcccgcgat cggcagcagc gcgtgagcga ttctcggacc caagtcacga
      901 gccggattga tcgcgtagcc ggtcggcccg cccaacgaaa ggccgatggc ccatacgaga
      961 atgccgacga ggaacgggcc gaatcccgca tcccaaccgt gcagcgggtt gagattcttc
     1021 ggtgtcagga tggccagcac ccccagcacc agcacgaacg tgccgatgac ctcggtcagg
     1081 agattcgcgg ccgggtcgcg gatggcaggc ccggtgcaaa acacggccag cttccctgcc
     1141 tggctttcgg tcacggccca gtgcggcaag taggcgagcc agacgatggt ggccccgagc
     1201 aacgcgccga gcatttgggc cgcgatgtag ccgggcaccg aggcccacgg gaacttgccg
     1261 atcgaggcga ggccgatggt caccgcgggg ttcaagtgtg cgccgctgaa ggcgcccacg
     1321 gcatacaccg cgaccgccac gccgaacccc cagccggcgg tgatgacgat ccacccggcg
     1381 ttctggcctt tggtcttgtt gagcacgacg ttggcgacca cgccatcgcc caggacgatc
     1441 aacagcatgg tgccgagcag ttctgcgagg aatgggggca taggcttgga tgaggaaggg
     1501 gcgaagctat cagcggagca gcgacttgtc cacacgctca gcggaagttc tcgatcaaca
     1561 tgatatcgct ttcaacctgg tctaaacggt cgaagagaat ccactgccca tcgggagaga
     1621 gatcaaagta ctgggcgcca ggggccccgg gtccgaggtc catcgaagta atggttctca
     1681 cgcgttgggt tgccaagtcc aagagtctga cgcgggcggg gatcgttgac ccgtcaatga
     1741 agtaaatgcc atcacgagta attttccacc agacaaacag ctgcacgcct gggaggacgc
     1801 ggacgggctc cgcgcccggg agccgcattt tccaaatagt ccctccctgt acccacacgt
     1861 agagagactt ggcatcggga gattcctggg cgctgaagcc accgctcttg gtaacttgta
     1921 cagcactgcc acccccggac ggtactttcc aaatctgcca attccccgtc cggctcgagg
     1981 aaaaatagat ccatttgcca tctcgcgacc acgtcgggac attattgtcg accggatcgc
     2041 tggtgagacg gcggggagcg cctccatcgg cactgataac gaagatatca ccgtggcctt
     2101 cgagccgggc atcaaacgca atggtctttc cgtccggaga ccaactcggc gagcctgcat
     2161 cggtcgcctt caaagacgta agctgtgcct ggttggtgcc gtcgctattg caagtccaga
     2221 tttccatact cccagagcga tcggatgcga acgcgatccg cttaccatcg ggagcatagg
     2281 cggcctcata gtccgcccgt gatgaagcaa ccattttcat cggaggggga gggcggcctt
     2341 tccacacagg tccgcgtgtt cgccagaggt tcgcatcgtc acggccattg atataggcta
     2401 gccgatctcc cttggtagaa atggtcggcg agctcgcgcc ctcacccacc ccgttcacca
     2461 gcgccggcgt gccccctgaa gaagagatgc gccacaatct catcaggccg ccacgatttg
     2521 aggtgaatac aatttctttt ccgtctggcg tccaggcgag accggccagg aatcgtccgt
     2581 cggaagtcag gcgacgggat ccgccaccgc tggcaggcac gacataaatg tcactggcaa
     2641 taaagcctgc tcctccggca taggcgatcg ctgttccatc gggggagaga attggatcgg
     2701 cgacatagag atcgggctgg gagacgaata ccttccgttg tccgtcctca accgacaaga
     2761 gcaggatgct ggagcgggac tcctcaggcg ccattctatc cgccacgatc aggtacttgc
     2821 cgtcgagcga ccagtcgatg caccgcccag agacctggct tcggtcatat gcctccccaa
     2881 tcttgcgttc ggcccccccc aacgctggga tcaggtagta agcagctctg tcactcgacg
     2941 gtccatcgcg aatgaaggcg atgtaacggc cgtcgggtga ccacgcgggg caatactccc
     3001 tggcgggact gctggtaagt cggagcggac cgcccgcgcc aatcagcttg acataaatgt
     3061 cggaattgtc gtcgttttcg cctctccaag cataggcaat ctggttgccg tccggcgaga
     3121 agacgggatg aaatttctga cctggcgaac tcgtgaaggg cacgacgttg gggtggtcct
     3181 gaccgacttg ggaactcttg gaagcataga accacgctgc agcgccaccg atgatgatca
     3241 ggactgccag tgcaccgccg gcggactgcc atcgggggcg caccgcccga gtcgagccgg
     3301 ccgtgagcgc tgcgacgaag gctcctgccg tggcgaatcg gtcggccggg gctttcgcga
     3361 gcgctttagt cacggcggct tccaccgccg gtgtaacgcg gcgaacggtg ccgaggtgcg
     3421 gtatcggttc ggtcaagcac ttggcgatga tggcctgcgc ggtcggaccg ttgtagggtg
     3481 gctctcccgc cagcatctcg tacaacacgc tcgccaggct gtactgatcg ctccggccat
     3541 cgagccgggg ctcggcgctc gcctgctcgg ggctcatgta cgccggcgtg ccgagggaga
     3601 gtcccgtctc ggtgagccgc tcccggcccg ccgtgctcac cgccagcgcg atcccgaagt
     3661 ctgccaccat gggctcgccc tggtggagca tgacattctc gggcttgata tcacgatgga
     3721 tgacgccgcg tccgtgcgcg aagtcgaggg ccgaggcgat cgcgcgggta atgctgagcg
     3781 cttcgtcgag gggcagctgg ccttcccgct ccagccgttg gcggaggctc tcgccctcaa
     3841 cgtacggcat cacgtaatag aggaagccgt ccgcctcgcc ggaatcgatg agtgtcaaga
     3901 tgtgcgtgtg ctggagctgc gccgtgaggc tgatctctcg gagaaaccgc tcaccgccga
     3961 gcacggcggc gagctcgggg cgcagcacct tgagcgcgac
//
LOCUS       DASQOP010000253         4502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1932833, whole genome shotgun sequence.
ACCESSION   DASQOP010000253 DASQOP010000000
VERSION     DASQOP010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1932833"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="VE030_10065"
     CDS             complement(<1..976)
                     /locus_tag="VE030_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HZF23797.1"
                     /translation="MATRKDLANAIRALAMDAVQKANSGHPGMPMGMAEIAEVLWNYH
                     LRHNPANPKWPDRDRFVLSNGHGSMLLYALLHLTGYALPLDEIRRFRQLHSMTPGHPE
                     YGMAPGVETTTGPLGQGLANAVGMAIAEKLLAAEFNRPGHDIVDHRTYVFVGDGCLME
                     GISHEACSLAGTLGLGKLTAIYDDNGISIDGHVSGWFRDDTPKRFEAYGWHVQRDVDG
                     HDVAAVDAAIIQAGRVADKPSLICCKTVIGMGSPNKAGSHETHGAALGDKEVAATREN
                     IGWKYPPFEIPQDIYAGWDARAKGAELETGWNRKFAAYAKAFPQEAGEF"
     gene            complement(1065..1259)
                     /locus_tag="VE030_10070"
                     /pseudo
     CDS             complement(1065..1259)
                     /locus_tag="VE030_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009935896.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(1524..1946)
                     /locus_tag="VE030_10075"
                     /pseudo
     CDS             complement(1524..1946)
                     /locus_tag="VE030_10075"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1983..2324)
                     /locus_tag="VE030_10080"
                     /pseudo
     CDS             complement(1983..2324)
                     /locus_tag="VE030_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107307.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(2321..>4502)
                     /locus_tag="VE030_10085"
     CDS             complement(2321..>4502)
                     /locus_tag="VE030_10085"
                     /inference="COORDINATES: protein motif:HMM:NF039586.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZF23798.1"
                     /translation="LRRSLMSRGMKLLITLVLLLGAVMLYLLSSVSADTPLFSGDLPL
                     LLGLGVGLVLVLMLVVGYQLLVLRRRLKARVFGSKLTLRLVLLFALVAVLPGALVYGV
                     SVQFLSRSIDSWFDVRVDKALEGGLSLGRVTLDNMLKELRAKAESMTLELTERSPQRR
                     LAALNTLREKAGVHEAALLGPDGSVMAFSGADPTGLVPELPSPSVVRKVRMQQPYSAI
                     EAVGDGGLQLRVLVPVNDVSGTTSLLQLVQPVPSHLARDAQTVQEIYRDYQQLSLSRQ
                     GLQRLFALTLTLSLLLALLSALLLAIVFSERLSAPLGILAAGTRAVAQGDFSPRHTVK
                     SDDELGLLTESFNTMTMQLADVRAEAQRNQEQLAAAKAYLESVLGNLSTGVMSFDSEF
                     RLRSANLSAVEIFGVDVGALIGTPPHEWGAREPRLAAVGQIVHEAFQRARPEVWEQQA
                     ELSGAAGTQVLLLRGSRLTAGAESGMVLVFDDITRLLQAQRLAAWGEVAQRLAHEIKN
                     PLTPIQLSAERLQRRLADKLSEQEAEILQRSTQTIVNQVAALKGMVDAFSQYARSPEA
                     DLQPLDLNQLIRDVLGLYESSRPALQLDLAGNLPHISGDAAKLRQVIHNLLQNAEQAV
                     AEVVVPRIVLRTDTARDGVRLTIQDNGPGFPEALMGRAFEPYVTTKAKGTGLGLAIVK
                     KIVEEHSGGIAIVNLPSSGASVTVTLPLAPAEAAALRANRQAGR"
BASE COUNT          804 a         1496 c         1429 g          773 t
ORIGIN      
        1 tgaactcccc ggcttcctgc ggaaaggcct tggcgtaggc ggcgaacttc cggttccagc
       61 cggtttccag ttcggcgcct tttgcccgcg catcccagcc ggcgtagata tcctgcggga
      121 tttcgaacgg gggatatttc cagccgatgt tctcccgggt cgccgcaact tccttatcgc
      181 ccagcgctgc gccgtgcgtt tcgtggctgc cggccttatt gggcgagccc atgccgatca
      241 ccgtcttgca gcagatcaga ctcggcttgt ccgcgactcg cccggcctgg atgatcgccg
      301 catccaccgc ggccacgtcg tggccgtcga cgtcgcgctg cacatgccag ccgtaggctt
      361 cgaaacgttt cggcgtgtca tcgcggaacc agccgctgac atggccgtcg atggaaatgc
      421 cgttgtcgtc gtagatcgcc gtcagcttgc ccaggcccag cgtgccggca agcgaacagg
      481 cttcgtgcga aatgccttcc atcaggcaac cgtcgccgac gaagacgtag gtgcggtgat
      541 cgacgatgtc gtggccggga cggttgaatt cggcggcgag cagtttttcc gcgatggcca
      601 tgccgaccgc attggcaaga ccctggccga gagggccggt agtggtctcc actcccggtg
      661 ccatgccgta ctcggggtgg ccgggagtca tcgaatgcaa ctggcggaag cgccggatct
      721 cgtccagcgg caacgcatag ccggtgagat gcagcaacgc gtacaacagc atcgagccgt
      781 gtccgttgga tagcacaaag cggtcgcgat cgggccactt gggattggcc ggattatggc
      841 gcagatggta attccacaag acctcggcga tctccgccat gcccatcggc atgccggggt
      901 ggccggaatt ggccttctgc accgcgtcca tcgccagcgc gcggatcgcg ttggcaagat
      961 ccttcctggt tgccatgact ccccttcgag aacacaaaaa atcccggcag accgggatcg
     1021 acaacgccgg tattttagcc cggatagtcc gtcggaacgc cgggctactc gtcggcgcgg
     1081 cgcggaagct tgatgccaag ctgcttgagc ttgcgataga ggtgggtgcg ctccaggccg
     1141 acccgatcgg cgacgcggct catgttgccg ccctcgcgac cgaggtgata ctcgaagtag
     1201 acacgctcga aggcatcgcg cgcctcgcgc agctcgcggt ccagcggcag cgactgcgtg
     1261 gggttgggta gcggggcctc gagcacggca aacgcgttga tgacttcggc aacgccgatg
     1321 ccggcgccga gcgcgagctg ggccacggtc ttcaccacgc tttcgagttg aatcaaattg
     1381 ccgggccaat ggtaattgcg cagggcattc agcgccgcag tattgaacgc tcgtagcggc
     1441 gtttctttcg cctcgatcgc ccgcgacagc accagattgg caagctcggg aacgtcctcg
     1501 gcgcgctcgc gcagcgcggg cacgcgcacg atcacgccgg ccagcagcgc atatagcctc
     1561 ggatcgaagg cgccgcccgc caccaattcc gccagcggcg ccgaagtcga ggagacgagg
     1621 cgcgcgttgt agcgatccag tttgagcgtc tgtcccagca gactcttctg ctgactgcgc
     1681 gaaagcgtgt cgatgtctcg caggtacagg atgccctcgg ccgcctgtcc gaccagattg
     1741 ttctccgcct gatcgaatac tgccagatcc tccggtgcca cccatgcggc gttggactgt
     1801 tgcagcaggc gcgcgcactg ctccatcccg gtccccggtt cgccgacaaa aagcgcgggc
     1861 gttttcaagg cgcgcaaacg ctcgatctgg gcgcgcaact gttccacagc acgcgacttg
     1921 cccgcggtaa ccagcaccgc cggcacgccg ggcctgggcg cgccctgctt gagcgccgtg
     1981 ccgacggtgg aaagcagttt ctgcaaagcg atcggctttt ccaggaaggc ataggcgccg
     2041 atacgggtcg cctctaccgc ggtgtcgatg gtgccatggc cggacatcat taccaccggc
     2101 atggtgagct ggccggtgct cgcccattcc ttcagcagcg aaatgccgtc ggtgtcgggc
     2161 atccagatgt cgagcagcac cagatccgga cgcacgcggc cgcggatctg ccgtgcctcg
     2221 ctggcgttct gcgccagtcg cacctggtgg ccctcgtcac gcagaatttc cgagagcagc
     2281 tcgcggatac cgacttcgtc gtccactacc agaatctggc tcatctgccg gcctgcctgt
     2341 tggcccgcag cgcggccgcc tcggccggtg ccaacggcag tgtcacggta acgctggccc
     2401 cagacgatgg caggttgacg atggcaatgc ctccgctatg ttcttccaca atcttcttga
     2461 cgatggccag cccgagtccc gtgcccttgg ccttggtggt gacatagggc tcgaacgccc
     2521 ggcccatcag cgcctcggga aagcccggac cgttgtcctg gatggtgagc cgcacgccgt
     2581 ctcgagccgt atcggtgcgc agcacgatgc gcggcacgac gacttccgcc accgcctgct
     2641 ccgcattctg cagcaggttg tggatgacct ggcgcaactt cgcggcatcg cccgagatgt
     2701 gcggcaaatt gccggccagg tcgagttgca atgccgggcg cgacgattcg tacagcccga
     2761 gtacgtcgcg gatgagctga ttcaggtcca agggctgcag gtcggcctcg ggtgagcgcg
     2821 cgtactggct gaacgcgtct accatgcctt tgagcgccgc cacctggttg acgatggtct
     2881 gggtggagcg ctgcaggatt tcggcttcct gctcgctgag tttgtcggca agccgccgct
     2941 gcaaccgctc cgccgaaagc tggatcggcg tgagcgggtt cttgatctcg tgagcgagcc
     3001 gctgcgccac ctcgccccag gctgcgagcc gctgcgcctg cagcaaacgg gtgatgtcgt
     3061 cgaaaaccag caccatcccg gactccgcgc cggcggtgag ccgcgagcca cgtaacagca
     3121 atacttgcgt gcccgcggcg ccggacagct cggcctgctg ttcccagacc tccgggcgcg
     3181 cccgctggaa ggcttcgtgc acgatctggc cgaccgcagc gagccgcggc tcgcgcgccc
     3241 cccattcatg cgggggcgtg ccgatcaggg cgccgacgtc cacgccgaag atctcgaccg
     3301 cgctgagatt ggcggagcgc aaccggaatt ccgagtcgaa tgacatcacg cccgtcgaca
     3361 gattaccgag cacgctttcg aggtaggcct tggcagcggc gagctgctcc tggttgcgct
     3421 gcgcctcggc gcgtacgtcg gcaagctgca tggtcatggt attgaatgat tcggtcagaa
     3481 gacccagctc gtcgtcgctc ttgaccgtgt ggcgcgggct gaaatcaccc tgcgcaaccg
     3541 cgcgcgtgcc ggcggccagt atgcccagcg gcgcggacaa acgctcgctg aacacgattg
     3601 ccaggagcag cgcggagagc agcgccagca gcagcgacaa tgtcagcgtt agcgcaaaaa
     3661 gacgctgcag accctggcgc gacagcgaca gttgctggta atcgcggtag atttcctgaa
     3721 cagtttgcgc gtcgcgcgcc aagtgcgaag gcacgggttg caccaactgc agcaacgagg
     3781 tggtgccgga cacatcgttc accggcacca gcacccgcag ttgcagcccg ccgtcgccga
     3841 cagcctcgat ggcgctgtaa ggttgctgca tgcgcacctt ccgcaccacc gacggcgagg
     3901 gcagttccgg aaccagacca gtggggtccg cgcccgaaaa agccatcacc gagccgtcgg
     3961 ggccgagcaa tgccgcctcg tgcactccag ccttttcgcg cagcgtgtta agcgcggcca
     4021 gacgccgctg cggcgagcgc tcggtaagtt caagagtcat cgactcggct ttggcgcgta
     4081 attccttgag catgttgtcc agcgttaccc ggcccaggct caagccgcct tccaacgcct
     4141 tatcgacccg cacgtcgaac caggagtcga tcgaccgcga caaaaactgc accgacacac
     4201 cgtacacgag cgcgcccggc aacaccgcca ctaaggcgaa caacagcacc agccgcagcg
     4261 tcagcttgga gccgaatacc cgcgccttga gacgccggcg cagcaccagc agttggtagc
     4321 cgaccaccag catgagcacc agcaccagtc caacgcccag gcccagcagc agcggcagat
     4381 cgccggagaa cagcggcgtg tcggcgctga cgctggaaag caggtacagc atgaccgcgc
     4441 cgagcaacag taccagcgtg attagtagct tcataccgcg gctcatcagc gaacgcctga
     4501 gg
//
LOCUS       DASQOP010000254         2443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_125021, whole genome shotgun sequence.
ACCESSION   DASQOP010000254 DASQOP010000000
VERSION     DASQOP010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125021"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VE030_10090"
     CDS             complement(<1..625)
                     /locus_tag="VE030_10090"
                     /inference="COORDINATES: protein motif:HMM:NF044960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23799.1"
                     /translation="MTVSLRYQVMQTIFETPNASAPLEKIALETYVPPAKPSLVGLSR
                     AEIAERVGAIGVAEAQRKMRVQQLWHWIYVRGARNFDEMTSISREMRTQLDAHFTVER
                     PEVVAEQVSSDGTRKWLLRLPSGDANEKAHEVECVYIPETDRGTLCVSSQVGCTLNCT
                     FCHTGTQRLVRNLTSGEIVGQIMVARDRLNDWADRVTPTGSRLVTNVV"
     gene            complement(770..1063)
                     /locus_tag="VE030_10095"
     CDS             complement(770..1063)
                     /locus_tag="VE030_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZF23800.1"
                     /translation="MNQKSGTARSPSAQGFTEKQGQYLAFIYTYSHIFRRPPAETDMQ
                     RHFQVSPPSVHQMIVTLERNGLIRRQPGVARSIQILVAPKDLPILNWLEINQS"
     gene            1101..2222
                     /locus_tag="VE030_10100"
     CDS             1101..2222
                     /locus_tag="VE030_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HZF23801.1"
                     /translation="MNVRYRVELSQAERCELTAMLSKGKRAARKLKRAQILLAADAGR
                     SDEEIARAVAVGGSTVYRTKRRFVEGNLERALSEESRPGAERKLTGKEEALLVATACA
                     NPPEGRARWTLELLADAMVKLTGHESLSGETVRRRLAENGLKPWRKDMWCIPKVDGEY
                     VARMEDVLDLYAETPDPKRPVVCFDESPVQLIGEVRQPIPAKPGQLERYDYEYRRNGT
                     VNLFVVLDVNQPWRKVKVTARRTAEDYAQCMRELVDVHYPDAACIRVVQDNLSTHSAG
                     SLYEAFTPAEARRILRRIEFHYTPKHASWLNMVEIEIGVLRGQCLDRRIDDPERLVRE
                     IAAWERQRNAEGARIKWMFTTEKARTKMGRAYPVTSKES"
BASE COUNT          474 a          733 c          772 g          464 t
ORIGIN      
        1 tcactacatt ggtgacgaga cggctgccgg tcggggtgac gcggtcggcc cagtcattga
       61 ggcgatcgcg tgccaccatg atctggccga cgatttcgcc tgacgtgaga ttgcgcacca
      121 gccgttgcgt gccggtatgg cagaacgtgc aattcagggt gcagccgacc tgcgaggaga
      181 cgcacagcgt gccgcggtcg gtttccggaa tatagacgca ctcgacttca tgcgccttct
      241 cgtttgcatc gccgctcggc aatcgtaata gccatttgcg ggtgccgtcg ctggaaacct
      301 gctccgcgac cacttcggga cgctcaacgg tgaaatgcgc atcgagttgg gtgcgcattt
      361 ccctggaaat gctggtcatc tcgtcgaaat tcctggcgcc acggacatag atccagtgcc
      421 acagctgctg cacgcgcatc ttgcgctgcg cctcggccac gccgatggcc cccacccgtt
      481 ccgctatctc cgcgcgcgac agcccgacca gcgagggctt ggccggcggc acgtaggttt
      541 caagcgcgat cttttccagg ggcgctgacg cattgggcgt ttcgaaaatc gtctgcatta
      601 cctggtatct caatgaaacc gtcatgcccg ggcttgtccc gggcatccac gtccttttgc
      661 ttcgccggcc aagacgtgga tagccgggac aagcccggcc atgacgacca agaacgtgtc
      721 ttatatagca gccggcagcg caattgcgac tagttcctcg tcacagtgat tatgactggt
      781 tgatttccag ccaatttagg atgggcaggt ctttcggagc aacgaggatt tggatgcttc
      841 gggcgacgcc gggttgacgg cgaatgaggc catttcgttc gagcgtcacg atcatctggt
      901 ggaccgaggg cgggctgact tgaaagtggc gctgcatgtc ggtctcggcg ggtgggcgtc
      961 ggaaaatgtg cgagtaagtg tagatgaaag ccaggtactg gccctgcttt tcggtgaagc
     1021 cttgagcgct ggggctgcga gcggtgcctg atttttgatt catttgtcga ttcgtccggt
     1081 cctccgacaa ggaggtcgag atgaatgtac gttaccgggt cgaattgagc caagccgaac
     1141 gctgcgaact caccgcgatg ttgagcaaag gcaaacgggc cgcccgcaaa ctcaagcgcg
     1201 cccagatatt gctggcagcc gatgccggcc gcagcgatga agagattgcc cgggcggtgg
     1261 cggtgggcgg ttcgaccgtg taccggacca agcggcgttt cgtggaaggc aatctggagc
     1321 gggcgctgag cgaggagtcg cgtcccggcg cggagcgcaa gcttacgggc aaggaggaag
     1381 ccttgctggt ggcaacggct tgcgcaaacc ctcccgaggg tcgcgcccgc tggacgctgg
     1441 aacttttggc tgacgccatg gtcaagctca ccggacacga gagcctgtcg ggcgagacgg
     1501 tgcgccggcg gctggccgaa aacggtctca agccctggcg caaggacatg tggtgcattc
     1561 caaaggtcga tggcgaatat gtcgcccgca tggaagacgt gctcgacctc tatgccgaaa
     1621 cgccagatcc caagcgaccg gtggtctgct tcgacgaaag cccggtccaa ctcatcggcg
     1681 aggtccgcca gccgatcccg gccaagccgg gccaactcga acgctacgat tacgagtatc
     1741 gccgcaacgg caccgtcaac ctctttgtcg tgctcgacgt caatcagcct tggcgtaagg
     1801 tcaaagtcac cgcgcggcgc accgccgagg attacgccca atgcatgcgc gaactcgtcg
     1861 atgtccatta tcccgacgcc gcgtgcatcc gggtcgtgca ggataatctg tcgacccact
     1921 cggcgggctc cctctatgag gcattcacac ccgccgaggc ccggcgtatc ttgcgccgca
     1981 tcgagttcca ctacacaccc aagcacgcca gctggctcaa catggtcgag atcgagatcg
     2041 gcgtcctgcg cggtcagtgc ctggaccgta gaatcgacga ccccgagcgc ctcgtccgcg
     2101 aaatcgccgc atgggagcgt cagcgaaatg ccgaaggagc ccgcatcaaa tggatgttca
     2161 caaccgaaaa ggcccggacc aaaatgggcc gcgcctatcc cgtaacgtcc aaagagtcat
     2221 aatcactgtg tagaggtact agtatgccgt ctcctgattt tgggggtgga catgcagcgc
     2281 cgcgctttca tcaccctttt taccggcgcg gcagtcgcgc ggccacttgc ggcgcgcgcg
     2341 cagcagaccg agcggatgcg gcggatcggc gtgctcatga gcgccgcggc tgatgatccg
     2401 gaaggacagg cccgcgtcac tgtgttcgtg caggcgtcaa aag
//
LOCUS       DASQOP010000255         6825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2942390, whole genome shotgun sequence.
ACCESSION   DASQOP010000255 DASQOP010000000
VERSION     DASQOP010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6825
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2942390"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /gene="guaA"
                     /locus_tag="VE030_10105"
     CDS             <1..1018
                     /gene="guaA"
                     /locus_tag="VE030_10105"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HZF23802.1"
                     /translation="YARFVRDICAAKPDWNMPDYIAEAVAKIHAAVGKDEVILGLSGG
                     VDSSVAAALIHRAIGGQLTCVFVDTGLLRLNEPAQVMETFADHMHMKVIHVDASDRFL
                     EQLAGVTDPEAKRKIIGREFVNVFQREAAKLPQVKWLAQGTIYPDVIESAGAKTKKAH
                     NIKSHHNVGGLPDTLHLKLLEPLRDLFKDEVRELGVALGLPHAMVYRHPFPGPGLGVR
                     ILGEVKREYADLLRRADAIFIEELRATVDADGQSWYDRTSQAFAVFLPVKSVGVMGDG
                     RTYEYVVALRAVQTQDFMTAHWAELPHALLSRVSNRIINEVRGINRVVYDISGKPPAT
                     IEWE"
     gene            1090..1551
                     /gene="tadA"
                     /locus_tag="VE030_10110"
     CDS             1090..1551
                     /gene="tadA"
                     /locus_tag="VE030_10110"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HZF23803.1"
                     /translation="MPDEDFMRAALALAEQARGAGEVPVGAVVERDGVVIGSGFNSPI
                     GRHDPSAHAEIVALRAAAAALGNYRLKGCTLYVTLEPCIMCSGAILHARIGRVVYGAP
                     DPKTGACGSVLNLFAQGRLNHHTAVEGGMLAEECGRLLKSFFQERRGANGQ"
     gene            1548..2039
                     /locus_tag="VE030_10115"
     CDS             1548..2039
                     /locus_tag="VE030_10115"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HZF23804.1"
                     /translation="MKSIIVQVPAQTLELFDGGSLLRRYVVSTSDRGTGEKNGSFCTP
                     RGRHLVRARIGAGAPLNTVFVKRRPTGEMYTAELAARFPGRDWILTRILWLSGCEPGF
                     NRLGEVDTMRRYIYIHGSPDSAAMGSPGSIGCIRMHNRDIVELFDLIEAGTPVDILDL
                     RPV"
     gene            2121..2861
                     /locus_tag="VE030_10120"
     CDS             2121..2861
                     /locus_tag="VE030_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP reductase"
                     /protein_id="HZF23805.1"
                     /translation="MRLKDKVCIITGSARGIGHATALKFAREGARVVVCDLDRKGVDA
                     VVAEIENGGGEAIGFVVNVVDKASIAAMVKGVMARYGRIDTLVNNAGIVEDSQFRKMS
                     DEQFDRVIDVNLKGTYNCTRAVVDVMLAQTSGVILNASSVVGLYGNFGQTNYAASKFG
                     VIGMAKTWARELGRKGIRANAVCPGFVETPILKSIPEKVMRALIERVPLGRLAKPEEI
                     ANTYAFLASDEASYINGAVIEVGGGATI"
     gene            2898..3326
                     /locus_tag="VE030_10125"
     CDS             2898..3326
                     /locus_tag="VE030_10125"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZF23806.1"
                     /translation="MAAHQLRVRAADWNVDRAALRAIREQVFVREQAVPVELEWDEFD
                     PLCQHVVAEAAGQAIGTGRLLPDGHIGRMAVLESWRGQGVGSALLVALLRLARDRGIR
                     RVRLNAQSRAVAFYARHGFVAEGEEFIEAGIPHRSMWRDV"
     gene            complement(3323..4120)
                     /locus_tag="VE030_10130"
     CDS             complement(3323..4120)
                     /locus_tag="VE030_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HZF23807.1"
                     /translation="MCLILIAHRAVPGYALVLAANRDEFFARPTEPADYWADAPQVLA
                     GRDLEKGGTWMGATRDGRWAAVTNYRDGTRPETGSRSRGELVARYLLERSSAAAYAAA
                     VAGAGAEYHGFNLLVGDAEGVHYVSNRGARPQALQPGIYGLSNHLLDTPWPKVERGKR
                     ELEALLRGNPKDPSEPLLALLADRRPAPTEALPETGVSRDWEKLLSSAFIRAPGYGTR
                     ASSVLLIEHGGEASLHERSFGASAELLEDRRFRFTVAPTPSPLATVN"
     gene            complement(4111..4395)
                     /locus_tag="VE030_10135"
     CDS             complement(4111..4395)
                     /locus_tag="VE030_10135"
                     /inference="COORDINATES: protein motif:HMM:NF027614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4936 family protein"
                     /protein_id="HZF23808.1"
                     /translation="MTLCYYVYYRVTQPQQAGAAVRDMQSQLLVRSGVRGRLLIKRDQ
                     PGLWMEVYEGVNDAPAFEAELDRLIAATKLEGFLAPGSRRHSECFEESCA"
     gene            complement(4399..5403)
                     /locus_tag="VE030_10140"
     CDS             complement(4399..5403)
                     /locus_tag="VE030_10140"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HZF23809.1"
                     /translation="MGHPYDWNQIADAPHGAPHAHFGDPRRELEAARSGAVICPLSHF
                     ELLRFSGVDAQSFLQGQLTCDVLQVTPTAARYGGYCTPKGRLIADFLLMRTPQAYLMH
                     LPADSWAMLAERLRKFVLRTKVEIGRETTLRAIGIAGPEAVALLRQSVGEPPRLALES
                     VQYASATLLRLPGAAFLAIAPQPDMAGLWDLLARQATPAGMDCWNWLQIHAGIPWISA
                     ATREQFVPQMVALDAIGGVDFQKGCYPGQEIVARTHYLGEVKRALRFGHGEGPATAGD
                     VLLRADGLSVGMVVNAAPAPDGGTDLLAVVQTDTDGEQPRLRSADGPAVRISQPSGAR
                     "
     gene            5461..5907
                     /locus_tag="VE030_10145"
     CDS             5461..5907
                     /locus_tag="VE030_10145"
                     /inference="COORDINATES: protein motif:HMM:NF018908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HZF23810.1"
                     /translation="MLRIAIAPSPLLAVLLVIVHLIAGACVLILLPARWAAAILIAVL
                     CASLIFHLRRDALRLSGDAVIEVTLRERGRCELLTRGGAVLEGTVAGSSFVSALFTVI
                     NVRLDGGRKLRSTVVMPDSVPAEERRRLRVWLRYRAHPEMPDSPSL"
     gene            complement(5893..>6825)
                     /locus_tag="VE030_10150"
     CDS             complement(5893..>6825)
                     /locus_tag="VE030_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZF23811.1"
                     /translation="DGIAMGWRAGCTVANMEFIQFHPTCLYHPHAKSFLISEAVRGEG
                     GVLRLADGRRFMPAHDQRAELAPRDVVARAIDFEMKKRGLDCVYLDITHKPAAFLKEH
                     FPNIFARCLELGIDITRQPIPVVPAAHYTCGGIQTDKNACTDLAGLYAVGETAHTGLH
                     GANRLASNSLLECLVFGQAAARHILGQQRPNSPTLPQWDESRVTDADEEIVISHNWDE
                     LRRFMWDYVGIVRTNKRLQRAQHRIRLLTEEIQEYYSNFRVTADLLDLRNLVATADLI
                     VRSAMLRRESRGLHFSRDYPRQLSVARDTVLQGG"
BASE COUNT         1200 a         2141 c         2287 g         1197 t
ORIGIN      
        1 ctacgcccgc ttcgtacgcg acatctgtgc cgccaagccc gactggaaca tgcccgacta
       61 catcgccgag gcggtcgcga aaatccatgc ggcggtgggc aaggacgagg tcatcctggg
      121 gctgtccggc ggggtcgatt cgtcggtcgc agcggcgctg atccaccgcg ccatcggcgg
      181 gcagttgacc tgcgtgttcg tcgataccgg cctgttgcgg ctcaacgagc ccgcgcaggt
      241 gatggaaaca ttcgccgacc acatgcacat gaaagtcatc cacgttgacg cgtcggaccg
      301 gttcctcgaa caactggcag gggtcaccga tcccgaggcc aaacgcaaaa tcatcgggcg
      361 cgagttcgta aatgtattcc agcgcgaggc ggcgaaactg ccgcaggtca aatggctggc
      421 ccagggcacg atctatccgg acgtgatcga gtcggccggc gccaagacca agaaggcgca
      481 caacatcaag tcgcaccaca acgtcggcgg cttgccggac acgctgcact tgaagctgct
      541 ggagccgttg cgcgacctct tcaaggatga agtgcgcgaa ctcggcgtgg cgctgggact
      601 gcctcacgcg atggtctatc gccatccttt ccccggtccg ggactggggg tgcgcatcct
      661 gggcgaggtc aagcgggagt acgccgacct gttgcgccgc gccgacgcta ttttcatcga
      721 ggagttgcgt gccaccgtcg acgccgacgg gcagagctgg tacgaccgga cctcgcaggc
      781 attcgcggtg ttcctgccgg tgaaatcggt cggcgtcatg ggcgacggcc gcacctacga
      841 gtacgtggtg gcgctgcggg cggtccagac ccaggatttc atgaccgcgc actgggccga
      901 gttgccccat gcgctgcttt ccagggtgtc caaccgcatc atcaacgagg tccgcggcat
      961 caaccgggtc gtatacgaca tttccggcaa gccgccagcc accatcgagt gggaatgacc
     1021 cggctggatc agcccgtcaa tacttgaact tgtttctcgg gtatatttga taagtatagg
     1081 attttaaata tgcctgatga ggatttcatg cgtgcggcgc tggcgcttgc ggaacaggcg
     1141 cgaggcgcgg gagaggtgcc ggtcggagcc gtggtagagc gggacggcgt tgtcatcggc
     1201 agcggcttca attccccgat cggccgccac gatccctcgg cgcatgcgga gatcgtggcg
     1261 ctgcgcgcag cggcggcagc gctgggcaat taccggctca aaggctgcac gctttacgtc
     1321 acgctcgagc cctgcatcat gtgctcggga gcgatcctgc atgcgcgcat cggcagagtg
     1381 gtctacgggg cgccggaccc gaagaccggt gcctgcggca gcgtgctgaa tctgtttgcc
     1441 caaggcaggc tcaatcacca caccgcggtg gagggcggaa tgctcgctga ggaatgcggc
     1501 cgcttgttga agagcttctt tcaggagcgc agaggggcga acggacagtg aaatccatca
     1561 tcgtgcaggt gcctgcgcag acgctggaat tgttcgacgg cggcagcctg ttgcggcgct
     1621 acgtggtttc gacttctgac aggggcaccg gggaaaagaa cggcagtttc tgcacgccac
     1681 gcggccggca tctggttcgg gccagaatcg gcgccggcgc cccgctcaat accgtgttcg
     1741 tcaagcgccg ccccaccggc gagatgtata ccgctgaact ggcggcgcgc tttcctggtc
     1801 gcgactggat tctcacccgt atcctttggc tgtccgggtg tgagccgggc ttcaatcgcc
     1861 tgggcgaggt ggataccatg cgccgctaca tctacatcca cggcagcccg gattcggcgg
     1921 cgatgggcag ccccggctcg atcggctgca tccgcatgca caaccgcgac atcgttgaac
     1981 tattcgatct catcgaagcc gggactccgg tcgatatact cgatttgcga ccggtgtgaa
     2041 ggcggctttc gtagctgccg ggacacctcg cagcacgatg cactggcgga cccaaaccaa
     2101 ccgacaagga gactcaacca atgcggctga aggataaggt ttgcatcatc accggctcgg
     2161 cgcggggaat tggccacgcc accgccctca agtttgccag ggagggggcc cgggtggtgg
     2221 tttgtgacct cgaccgcaaa ggtgtggacg cggtcgtggc ggagatcgaa aacggcggcg
     2281 gagaggcgat cgggttcgtg gtcaacgtgg tcgacaaggc cagcatcgcc gccatggtga
     2341 agggcgtgat ggcccgctac ggccgcatcg acacgctggt gaacaacgcc ggaattgtcg
     2401 aggactcgca gttccggaaa atgtccgacg agcagttcga ccgcgtcatc gatgtcaacc
     2461 tgaagggcac gtataactgc acccgcgcgg tcgtggacgt gatgctggca cagacttcgg
     2521 gggtgatcct gaatgcctcg tccgtcgtcg gcctgtacgg caacttcggc cagaccaact
     2581 acgcggcgag caagttcggc gtaatcggca tggccaagac ctgggcgcgc gaactgggtc
     2641 gcaagggcat tcgtgccaat gccgtgtgtc ccggtttcgt cgaaaccccg atcctgaagt
     2701 ccatcccgga aaaggtgatg cgggcactga tcgagcgcgt gccgctgggt cgcctggcga
     2761 aaccggagga aatcgccaat acctacgcct tcctggcctc cgacgaggcc agttacatca
     2821 acggcgcggt gatcgaagtg gggggcggcg cgacgatcta accgcaacct tgcaggcctg
     2881 ctcgaccgcg acgattcatg gctgcgcatc agctcagggt ccgtgccgca gactggaacg
     2941 tcgaccgggc cgcgttacgc gccatacgcg agcaggtctt cgtgcgcgag caagccgtgc
     3001 ctgtcgagct ggagtgggat gaattcgacc cgctgtgcca gcacgtcgtg gccgaagccg
     3061 cgggccaggc gatcggcacc ggacggctgc tgccggacgg acacatcggg cgcatggcgg
     3121 tactggagtc atggcgcgga cagggcgtgg gcagtgcgtt actcgtggcg ttgctgcgac
     3181 tggcgcgcga ccgcggcatc cggcgggtga ggctcaacgc gcagtcccgg gcggtggcgt
     3241 tctatgcgcg ccacggcttc gtcgctgaag gcgaagaatt catcgaggcc ggcattccgc
     3301 accgctcgat gtggcgcgac gtctaattca ccgtcgccag cggcgaggga gtcggcgcga
     3361 cggtgaagcg gaatcggcga tcctcgagca gttctgcgct ggcgccgaac gagcgctcgt
     3421 gcaaactcgc ttcaccgccg tgttcaatca gcaacaccga ggaggcccgg gtaccgtaac
     3481 ccggcgcacg gatgaaagcg gaagacagca atttctccca gtcgcggctg acgccggttt
     3541 cgggcaaggc ctcggtgggg gcgggccggc ggtcggcgag caacgccagc agcggctcgc
     3601 tcggatcctt cgggttgccg cggagcaagg cctccagttc gcgcttgccg cgctctacct
     3661 tgggccacgg cgtgtccagc agatgattgc tcagaccgta gatccccggt tggagcgcct
     3721 gcggccgcgc gccgcggttg gaaacgtaat gcacgccttc ggcgtcgccc accaggagat
     3781 tgaagccgtg atactcggcg cccgcgccgg ccacggcggc ggcataggcc gccgcggacg
     3841 acctttccag cagatagcgc gccaccagtt ccccgcgcga gcgtgatccg gtctcgggtc
     3901 gggtgccgtc acggtaattg gtaaccgccg cccaacggcc gtcgcgcgtc gcgcccatcc
     3961 aggtcccgcc tttttccaga tcgcggccag cgagaacctg cggcgcatcc gcccaataat
     4021 cggcgggctc ggtcggccgc gcgaagaact cgtcccggtt ggcggcaagg accaatgcat
     4081 agccgggaac agcccgatgc gcaatcagga tcaggcacat gactcttcga agcactcgct
     4141 atggcgccgg ctgccgggcg cgagaaaacc ctccagtttc gttgcggcga tcaggcgatc
     4201 cagctccgct tcgaatgccg gtgcatcgtt cacgccttcg taaacttcca tccacaatcc
     4261 cggttggtcg cgtttgatca gcagccggcc gcggacgccg ctacggacca gcagttggga
     4321 ctgcatgtcg cgcacggcgg ccccggcctg ctgcggctgt gtcacccggt agtagacgta
     4381 ataacagagg gtcaagattc agcgcgcgcc gctcggttgc gagattctca cggcggggcc
     4441 atcggcgctg cgcagccttg gctgttcgcc gtccgtgtcg gtctggacca ccgccagcag
     4501 gtccgtaccg ccgtccggtg ccggggcggc attgaccacc atgccgacgg acagtccgtc
     4561 ggccctcagc agcacgtctc cggccgtggc tggaccttcg ccgtggccga aacgcagggc
     4621 gcgctttact tcgccaaggt aatgcgtccg cgcgacgatt tcctgccccg gataacagcc
     4681 tttctggaaa tcgacaccgc cgatggcgtc cagcgccacc atctgcggca cgaactgctc
     4741 ccgggtggcg gcgctgatcc aggggatgcc ggcatggatc tgcagccagt tccagcagtc
     4801 cattccggcc ggagtggctt gccgggcaag caggtcccag agtccggcca tatccggctg
     4861 tggggcaatc gccagaaacg cggctccggg caggcgcaac agcgtggcgc tcgcatactg
     4921 aaccgattcg agcgccaggc gcgggggctc gccgaccgat tgccgcagca atgcgaccgc
     4981 ctccggaccg gcgatgccga ttgcgcgcag cgtggtttcg cggccgatct cgaccttggt
     5041 ccgcagcacg aacttgcgca atcgctcggc aagcattgcc cacgaatcgg cgggcagatg
     5101 catcaggtaa gcttgcggcg tgcgcatcag cagaaaatcg gcgatcaggc ggcccttcgg
     5161 cgtgcagtag ccgccatagc gcgccgcagt gggcgtgacc tgcagcacgt cacaggtcag
     5221 ttgcccctgc agaaaggatt gtgcgtcgac gccggaaaag cgtagcagtt cgaaatggct
     5281 caacgggcag atcactgcgc ccgaacgggc agcctcgagt tcgcgccgcg gatcgccaaa
     5341 atgcgcgtgc ggagcaccgt gcggggcgtc ggcgatctgg ttccagtcgt aggggtgtcc
     5401 catgattcaa gaatccgctt tcaggcgatt ataagtccac agtccgagga gagagaacgc
     5461 ttgctgcgca ttgccatcgc cccgtcgccc ttgctggccg tcctgctggt aatcgtgcac
     5521 ctcatcgccg gggcgtgcgt gctcatcttg ctaccggcgc ggtgggcggc tgcgattctg
     5581 atcgctgtgc tgtgcgcaag cctgattttc catttgcgcc gcgacgcact gcgactgtcg
     5641 ggcgacgccg tgatcgaggt gacgctgcgc gagcgaggac gctgcgaact gctgacccgc
     5701 ggcggtgcag tgctcgaagg gacggtagcg ggctcgtctt tcgtttccgc attgttcacg
     5761 gtgattaacg tacggctgga cggcgggcgc aagctgcgct cgacagtggt aatgccagac
     5821 agcgttccgg cggaagagcg gcgccggctg cgcgtctggc tgcgctatcg ggcgcacccg
     5881 gaaatgccgg attcaccctc cttgtaacac ggtgtcgcgc gccacggaga gttgccgtgg
     5941 ataatcgcgg ctgaaatgca ggccccggct ctcgcgccgc agcatggcgc tcctgacgat
     6001 caggtccgcg gtggccacca ggttgcgcag gtccagcagg tccgcggtga cccggaaatt
     6061 ggagtagtac tcctggatct cctcggtcaa cagacgaatg cggtgctgcg ccctttgcag
     6121 gcgtttgttg gtacggacga taccgacgta gtcccacata aaccggcgca actcgtccca
     6181 attgtgcgaa atcacgatct cttcgtcggc atcggtaacc cggctctcgt cccactgtgg
     6241 cagggtgggg gagttcggtc tctgctgccc gaggatatgg cgggcggcgg cctggccgaa
     6301 caccaggcat tccagcagcg agttgctggc gagccggttg gccccgtgca ggcccgtgtg
     6361 ggcagtctcg cccacggcgt acaggccggc gaggtcggta caggcgttct tgtccgtctg
     6421 tatgccgccg caggtgtagt gcgcggccgg aaccaccggg atgggctgac gcgtgatgtc
     6481 gattcccagt tccaggcagc gggcgaaaat gttggggaag tgttctttca ggaaggcggc
     6541 gggcttgtgg gtgatgtcca gatagacgca gtcaagaccg cgcttcttca tctcgaagtc
     6601 gatggctcgt gccacaacat cgcgcggcgc cagctcggcg cgctggtcgt gcgccggcat
     6661 gaatcgcctt ccatccgcaa gccgcaacac cccgccttcg ccgcgcaccg cttccgagat
     6721 cagaaaggac ttggcgtggg ggtgatacag gcaggtcggg tggaactgga tgaactccat
     6781 gttcgccacc gtgcaaccgg cgcgccagcc catggcgatg ccgtc
//
LOCUS       DASQOP010000256        10721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1535699, whole genome shotgun sequence.
ACCESSION   DASQOP010000256 DASQOP010000000
VERSION     DASQOP010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10721
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1535699"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1308
                     /locus_tag="VE030_10155"
     CDS             <1..1308
                     /locus_tag="VE030_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HZF23812.1"
                     /translation="DAATAPAAAAPAAAAPAAPPAPTEPVLVTNDKISAGDTAWMLTS
                     TALVLMMTIPGLALFYGGMVRKKNVLATLMQSFAITCLVTILWWLVGYSLAFTPGGTA
                     YLGSFSRAFMNGAVYLKDAGKTSVSHLGVTVPETVYAMFQLTFAIITPALIAGAFAER
                     MKFSAMLWFMGIWSIVVYAPIAHWVWEPSGWLAVKGVLDFAGGTVVHINAGIAGLACA
                     LALGKRVGYGKEPMAPHNLTLTLVGASLLWVGWFGFNAGSAVASDGRAGMAMAVTQIA
                     TAAAALAWMFSEWLVKGKPSVLGIASGAVAGLVAITPASGFVGPNASVIIGVAAGVLC
                     FYASSYVKKSLGYDDSLDAFGVHCIGGIVGALLTGALVSKDISGADGSVMVQLWGVGT
                     TLIYGFVVSFIILKVIDVTIGLRVPEDHEREGLDISLHGEKVE"
     gene            complement(1433..2080)
                     /locus_tag="VE030_10160"
     CDS             complement(1433..2080)
                     /locus_tag="VE030_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HZF23813.1"
                     /translation="MENRDKPDSAPAETRPGAVAGNGKSLDRYLGNVIRELRLKDNLT
                     IAEVAAQAGISRGMVSKIENGQVSTSLETLSKIGQALGVSLSHLFRHYNMPYGGAQLV
                     KKGEGMEVVRRGTRRGHTYHLLAYDQGPKKTFEPFLITMDDASEVFPTFEHPGTEFIH
                     MLEGRIEYRHGQHTYMLEPGDSLTFRGDIPHGPERLLELPIHFLSIIIYGGHGEE"
     gene            complement(2092..3456)
                     /locus_tag="VE030_10165"
     CDS             complement(2092..3456)
                     /locus_tag="VE030_10165"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HZF23814.1"
                     /translation="MNKPVELHKQVQREESASFDRKVIDYIHHAAATGLYEIRGLGAK
                     RRMPHFDDLVFLAASLSRYPLEGYREKCVTRTVLGTRYAKKPIELAIPITIAGMSFGS
                     LSANVKEALGRAATEMGTSTTSGDGGMTSEERQSSKTLVYQCLPSRYGFRPDDVRRAD
                     AIEVVIGQGAKPGGGGMLLGQKINPRVAKMRVLPEGVDQRSACRHPDWTGPDDLTIKI
                     QELREITDWEKPIYVKIGATRVFNDVKLAVHAGADVIVVDGMQGGTAATQTVFIENVG
                     IPTLAALRQAVDALEDLNMKGKVQLIVSGGIRSGADVAKALAMGADAVSIGQGILFAL
                     GCNHEAYMQKGKHHSAVADYGKLGTAPGFCHHCHTGKCPVGVTTQDPVLEQRLEPLVG
                     AKRVRNYLKTLNMELTTIARACGKQNVHHLEREDLVALTVEAAAMARVPLAGTDWIPG
                     AATA"
     gene            complement(3590..4309)
                     /locus_tag="VE030_10170"
     CDS             complement(3590..4309)
                     /locus_tag="VE030_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein glxC"
                     /protein_id="HZF23815.1"
                     /translation="MASVAPAPRTTADTVTFDLARQSLRELNRFLHHDLRAGVKKVRV
                     LNPDGAHNIAVGVDAPVEIEVDGHAGYYAAGMNKQARVLIRGNAGPGLAEGMESGVVH
                     VKGFASVSAGAAGHGGLLVIEGDASLRCGISMKGLDIVVGGSVGDFSAFMAQAGRLIV
                     CGDAGDALGDSLYEAVIYVRGKVSSFGADAHEEPMRESDYVALADLLGRAGLKHDPRQ
                     FRRVASKRELYHWNADANQEY"
     gene            complement(4297..5193)
                     /locus_tag="VE030_10175"
     CDS             complement(4297..5193)
                     /locus_tag="VE030_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010102786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase family protein"
                     /protein_id="HZF23816.1"
                     /translation="MCGIVGLLVKNPALREQLGELMLPMLIGMTERGPDSTGLAVFTA
                     PVADGRRKFSLYSGEGEFDWTAFERAARQALSDDSEVSVQANHGVLETAAPPPQVTGW
                     IRAVYPRLHVLSAGQFIDLYKDVGAPAEVATRYDFRNLTGTHLVGHTRMATESAVTPD
                     RAHPFTAGEDWCLVHNGSLSNPYQIRRKLEPLGIAFETDNDTEAACRYFEWRMREGDD
                     LETAIHKGFEELDGFYTFLMGTKDELALVRDAFACKPAVVAETDDYVAISSEFRSLAK
                     LPDVKNARIFEPVPEEVYVWRA"
     gene            complement(5344..6678)
                     /gene="glnT"
                     /locus_tag="VE030_10180"
     CDS             complement(5344..6678)
                     /gene="glnT"
                     /locus_tag="VE030_10180"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III glutamate--ammonia ligase"
                     /protein_id="HZF23817.1"
                     /translation="MSPQAARKFLKDHHVKYILAQFVDIHGAAKAKAVPVDHLDMVLN
                     EGAGFAGFALWGFGMGPHGPDYMAVGDLSTLSLIPWMPGFARLACYGHVNGKPYQYCS
                     RVVLKRQLDALAKRGLTLYTGIEPEFMLLKRAGDGSLAPVDDTDLLEKPCYDYKGLAR
                     SVPFLEAMVDSLRAVGIDVYQIDHEDANAQFEVNFTYGEALASADRLVLVKMAASEIA
                     KSMGMIATFMPKPFSNRTGTGAHFHISLGNGKVKNLFHDDKDKNGMGLSQMAYHFAGG
                     VLAHARALTAIVAPTINSYKRLVVGRALSGATWAPAYIAYGNNNRTACVRVPYGRLEL
                     RLPDGSCNPYLASAAILAAGMDGVDRKLDPGKPINENLYEWSPEKLKRAGIDVLPQNL
                     KEALDEFEKDKVLRTALGEDLAQEFLTLKRMEWVEFSRHVSDWERDRYVEFF"
     gene            complement(6708..7382)
                     /locus_tag="VE030_10185"
     CDS             complement(6708..7382)
                     /locus_tag="VE030_10185"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23818.1"
                     /translation="MNVKRTSPLHDQVATLNPRWGRVRGMRMAAALAGDETVARESVA
                     VVDLSCLDKAGVKGPEAAPWLEGLGIVVPAQANAWTGLPGSGVIARLGRGEFFLEDGA
                     AGDTAGRVRDALGAGIAGVYPVTRQDAGIALVGRRVNELLVQTCNVNFQELDSESRTV
                     VMTMMAGVAVLVIRQDHQNLPGFRIWCDPTYAPYLWETLTGIAAELDGGPIGTGRLLS
                     QAAKLE"
     gene            complement(7385..10288)
                     /locus_tag="VE030_10190"
     CDS             complement(7385..10288)
                     /locus_tag="VE030_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HZF23819.1"
                     /translation="MSARLPLVAGEWIDRSKKFGFDFESERFQGFAGDTVSSALLAAG
                     VRVLGRSFKYHRARSVLSMANHDVNAMFQDGARLNLRGDVEPLRDRMVLAAVNTFGGV
                     RRDRARIFDKLSAVLPVGFYYKAFHTPKAWFPFWERMFRRVTGLGELDFSTPHIRTPK
                     RYDFCDVLVVGAGPSGLCASLAAADAGAKVVLVDENARIGGSLTYQRGGSDDPAAQLR
                     NLASGISAHPNISVRTGTLAAGCYADNWVALVDAERMTKIRARAIIVASGVCEQPAVF
                     HNNDVPGVMLASAAQRLIYRYAVRPMQSAVVLTGNQDGYAAALDLIANGIRVEAVVDL
                     RRTDGGSAEEARVTEAGIRTLRGHCIYEARASGSMVGAAVICPLLTGGEADASRSETV
                     RCDGVLMSVGYAPAAALLHQAGAAMRFDVQVGQFVPASLPGGVFAAGRVNGVYALEDR
                     QRDGSRAGLEAAKYLGFPASAAPVVVRAAVAPSHPYPIFDHPQAKNFVDFDEDLQLKD
                     LANAVQEGFDNIELLKRYATIGMGPSQGKHSNMNAIRVLAKLLGTTPGEVGTTTSRPF
                     FHPVPMSHLAGRGLVPYRLTPLHSRHERAGAVFMQAGIWLRPEFYMTRGKTRLEAVRE
                     EAAAVRTGVGIIDVGTLGKMEISGPDAATFLERIYTGRFANMKVGMTRYALMLDDAGV
                     IIDDGVVARLGEQRFYFTTTTTGSANVYREMTRLNTMWGMEVGIVNVTGAYAAVNLAG
                     PRARDPLAGVTNLDLSAEAFPYLGVREAEIAGVPARLLRAGFVGELGYEIHVPADRAC
                     ALWDALMEAGGVHGIRPFGVEAQRLLRLEKGHIIIGQDTDGLTTPLEAAVDWAVKMDK
                     PYFVGQRSLKIVQRKNPKAKLVGFTLDSATEGKVPRECHLVIRDGKIAGRVTSIAWSP
                     AMEKYVGLAFVSPEIIANGGGFFIRMDGGQMVPARIAKTPFYDPAGDRQKLGA"
     gene            complement(10285..10536)
                     /locus_tag="VE030_10195"
     CDS             complement(10285..10536)
                     /locus_tag="VE030_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HZF23820.1"
                     /translation="MRCPINGLRPVSEFQYAGEIRPMPDADTCTDAQWADYVFNRNGA
                     PGIKKEWWLHLASGIWFVAERDTAADEVLRTYLYGEGPA"
BASE COUNT         1871 a         3530 c         3237 g         2083 t
ORIGIN      
        1 gacgcggcaa ctgcacctgc tgcggcagcc ccggcagccg cagccccggc agcccccccc
       61 gcacctaccg agccggtgct ggtcacgaac gacaagatca gtgccggcga tacggcgtgg
      121 atgctcacct ccactgctct ggtgctgatg atgaccattc caggtttggc gctgttctac
      181 ggcggaatgg tgcgcaagaa gaacgtgctg gcaacattga tgcagagttt tgcgatcacc
      241 tgcctggtga ccattctgtg gtggctggtc ggatacagtc ttgcctttac gcctggcggc
      301 accgcctatc ttggtagttt cagtcgcgcc ttcatgaacg gagccgttta cctgaaggac
      361 gcagggaaaa cctcggtctc gcatctcggc gtgacggtac ccgagacggt atacgcgatg
      421 ttccagctca ccttcgccat catcactccg gcgttaattg ccggcgcttt cgccgaacgc
      481 atgaaatttt cggcgatgct ttggttcatg ggcatctggt cgatcgtggt ctatgccccg
      541 attgcacact gggtgtggga acctagcggc tggcttgctg tcaagggcgt gctcgatttc
      601 gctggcggca cggtggtgca catcaacgcg ggtattgcgg gtctggcatg cgcgctcgcc
      661 ctcggcaaac gcgtcggcta cggcaaggaa ccgatggcgc cgcacaacct cacgctaacg
      721 ctcgtcggtg catcgctgct gtgggtgggc tggttcggct tcaacgcggg ttccgcagtg
      781 gcgtccgacg ggcgtgccgg catggctatg gcggtaacgc agatcgcgac agcagcagcc
      841 gcgctcgcct ggatgttctc cgagtggttg gtcaagggca agccgagtgt gctcggaatc
      901 gcctccggag cggtggcagg actcgtcgca attacgccgg catccggttt cgtcgggccg
      961 aatgcgtcgg tgatcatcgg cgtggccgca ggcgtcctct gcttctatgc ctcctcgtac
     1021 gtcaagaaat cgctcggcta cgatgactcc ctcgacgcgt ttggcgtgca ctgcatcggc
     1081 ggtatcgtcg gcgctttgct gaccggagct ttggtcagca aagacatcag tggcgccgat
     1141 ggtagcgtga tggtccagtt gtggggagtg ggtacgaccc tgatctacgg cttcgtcgtc
     1201 tccttcatca tcctcaaggt catcgacgtg acgatcgggc tgcgtgtgcc ggaagatcac
     1261 gaacgcgaag gtctggacat cagcctgcac ggcgaaaaag tcgaatgaca attgttaggt
     1321 ttcgggattt ttatcggtcc cggaccatgt aatctcctcc ggtaagcatg cttaccaatt
     1381 catcgggcgc cttcgggcgc ccgttttttt tcccggagaa ctgccagcgg gcttattcct
     1441 cgccgtggcc gccgtagatg atgatggaaa ggaagtggat cggcagctcg agcaggcgct
     1501 cgggcccgtg tgggatatcg ccgcggaagg tgagcgagtc gccgggctcg agcatgtagg
     1561 tatgctggcc gtggcggtat tcgatcctgc cctcgagcat gtggatgaac tcggtgccgg
     1621 gatgctcgaa ggtcggaaac acctccgagg catcgtccat ggtaatcagg aaaggctcga
     1681 aggttttttt cggcccctgg tcgtaggcga gaagatggta ggtatggccg cgccgggttc
     1741 cgcggcgaac gacttccatg ccttcgcctt tcttcaccag ctgcgcgccg ccgtagggca
     1801 tgttgtagtg gcggaacagg tgcgagagcg aaacccccaa tgcctgaccg atcttcgaca
     1861 gcgtttccag gctggtggag acctggccgt tctcgatctt ggaaaccatg ccgcgcgaaa
     1921 tgccggcctg ggctgccacc tcggctatgg tcagattgtc cttcagccgc aactcgcgaa
     1981 tgacgttgcc gaggtaacgg tcaagcgatt tgccgttgcc cgccactgca cccggccgcg
     2041 tttccgcggg agcgctgtcc ggtttgtcgc ggttttccat tgcggcgaag gttaagcggt
     2101 ggctgcgccg ggaatccagt cggttccggc cagcgggacg cgtgccatgg cggccgcttc
     2161 aacggtcagc gcaaccagat cctcgcgctc gaggtgatgc acgttctgct tgccgcaggc
     2221 gcgggcaatg gtggtgagtt ccatgttcag agtcttcaga tagtttcgca cgcgtttcgc
     2281 accgaccagg ggctcgagac gctgctcgag caccggatcc tgcgtcgtca cgcccaccgg
     2341 gcatttgccg gtgtggcaat ggtggcaaaa gcccggcgcg gtgccgagct tcccgtagtc
     2401 ggcgacggca gagtgatgct ttcccttctg catgtaggcc tcgtgattgc agccgagcgc
     2461 gaacaggatg ccctgtccga tcgagaccgc gtcggcgccc atggccaatg ccttcgccac
     2521 gtccgcgccg ctgcgtattc cgccggagac gataagctgc accttgccct tcatgttcag
     2581 gtcttcgagc gcgtccaccg cctggcgcag cgccgccagg gtgggaatgc cgacgttctc
     2641 gatgaatacg gtttgcgtgg ccgcggtgcc gccctgcatg ccatccacca cgatcacatc
     2701 cgcgccggca tgaaccgcca gcttcacatc gttgaacaca cgggtcgcgc cgattttcac
     2761 gtagatcggc ttctcccaat cggtgatctc gcgcagttcc tggatcttga tggtgagatc
     2821 gtcaggtccg gtccagtccg gatggcggca tgccgagcgc tgatcgacgc cttcagggag
     2881 cacgcgcatt ttcgccaccc gcggattgat cttctgcccg agcaacatgc cgccgccacc
     2941 gggtttcgca ccctgaccga tgacgacttc gatcgcgtcc gccctgcgca cgtcgtccgg
     3001 cctgaagccg tagcgcgagg gtaggcactg gtacaccaat gtcttcgaag actggcgttc
     3061 ctccgaggtc atgccgccat cgccgcttgt ggtcgatgta cccatctcgg ttgccgcgcg
     3121 gccgagcgcc tccttgacgt tggccgacaa cgaaccgaag ctcatcccgg cgatggtgat
     3181 cgggatcgcc agttcgatcg gcttcttcgc ataacgcgtc ccgagcaccg ttcgggtcac
     3241 gcatttctcg cggtagccct ccagcggata gcgcgacagc gacgccgcga ggaataccag
     3301 gtcgtcgaag tgcggcatcc ggcgcttcgc gcccagtccc cgtatctcat ataacccggt
     3361 cgcggccgcg tggtggatgt agtcgatgac cttgcggtca aagctcgcgc tttcctcgcg
     3421 ctggacctgt ttgtgcaatt cgactggctt gttcatgttt ggtcccttcg gaaccaggaa
     3481 cgagggacga ggggttcggg attggcttcg agcgcatgtc gcgctggaat gcctaaacct
     3541 tggtccctca agccctcgtc cctcgcccct catccctcgc ccctgattct caatattcct
     3601 ggttggcgtc ggcgttccag tggtagagct cgcgcttgga cgccacgcgc cggaactgcc
     3661 tcggatcgtg tttgaggccg gccctgccga gcagatcggc gagcgccacg tagtccgatt
     3721 cgcgcatcgg ttcctcatgc gcatctgcgc cgaaggaact cactttccca cgcacataga
     3781 tgaccgcttc atagagcgag tcgccgagcg cgtcaccggc gtcgccgcac acaatcagcc
     3841 ggccggcctg cgccatgaac gccgagaagt ctccgaccga accgccaacc acgatgtcca
     3901 ggcccttcat cgatatgccg cagcgaagcg acgcgtcgcc ttcgatcacc agcaggccgc
     3961 cgtgcccagc cgcgccggcc gataccgatg cgaagccttt cacatgcacc acaccggatt
     4021 ccatgccttc ggccagcccc ggtccggcgt tgccgcgaat cagcaccctg gcctgcttgt
     4081 tcattcccgc cgcgtaatag ccggcatggc cgtccacctc gatctccacc ggcgcatcca
     4141 cgcctaccgc gatgttgtgc gcgccatccg ggttaagcac gcgtaccttc ttcacgccgg
     4201 ctcgcaggtc atggtggaga aaccgattca actcccgcaa cgattgcctg gcgagatcga
     4261 aggtgacggt gtcggccgta gtccgcggcg ccggcgctac gctcgccata cgtatacctc
     4321 ctccgggact ggctcgaaga tccttgcgtt cttcacgtcc ggcagctttg ccagcgacct
     4381 gaactcggag gagatcgcga cgtaatcgtc ggtttccgcc acgaccgccg gcttgcaggc
     4441 gaacgcgtcg cgcaccagcg ccagttcgtc cttggtgccc atcaggaagg tgtagaagcc
     4501 gtcgagttcc tcgaacccct tgtggattgc ggtctccaga tcgtcgccct cgcgcatgcg
     4561 ccactcgaaa taacggcagg ccgcctcggt atcgttgtcg gtctcgaagg cgatgccgag
     4621 cggctcgagc ttgcgccgga tctgatacgg attggaaagc gacccgttgt gcaccaggca
     4681 ccagtcctcg ccggcggtga acgggtgcgc ccggtccggc gtcaccgccg attcggtcgc
     4741 catgcgggta tgcccgacca gatgggtgcc cgtgagattc ctgaagtcgt aacgggtcgc
     4801 gacttcggcc ggcgcgccaa cgtccttgta cagatcgatg aactggccgg cggagagcac
     4861 atgcagccgc ggatacacag cgcggatcca cccggtgact tgtggtggcg gcgcggcggt
     4921 ttccagcacg ccgtggttcg cctgaacgct gacctcgctg tcgtcactca gcgcctgtcg
     4981 cgccgcgcgc tcgaacgcag tccagtcgaa ttccccctca cccgagtaca ggctgaattt
     5041 gcggcggcca tcggcaaccg gcgcagtgaa cacggccaat ccggtcgagt ccggtccacg
     5101 ctccgtcatg ccgatcagca tcggcagcat cagttcgccg agctgctcgc gcagcgccgg
     5161 attcttcacc agcagtccca cgattccaca cataatcacc tcctaagtct caccacagag
     5221 acacagagtc acagagtcac agagaaaaag agacctccag ccaagaacac gcgcccatcg
     5281 cttatgacgg gtttctgttt ccgaatttct ccgtgtccct gtgcctctgt ggttaatcca
     5341 gtcttaaaaa aactcgacgt agcggtcgcg ctcccagtcg gagacgtgcc gcgagaactc
     5401 cacccactcc atgcgcttca gggtaaggaa ctcctgcgcc aggtcctcgc ccagcgcagt
     5461 gcgcagcacc ttgtccttct cgaactcatc caacgcttcc ttgaggtttt gcggcagtac
     5521 gtcgattcct gccctcttca gtttctccgg cgaccactcg tagaggttct cgttgatcgg
     5581 tttgccggga tcgagtttgc ggtccactcc atccatgccg gcggcaagaa tcgccgcgct
     5641 ggcgaggtaa gggttgcagg acccgtccgg cagccgcagt tccagtcggc cgtagggaac
     5701 gcgcacgcag gcggtgcggt tgttgttgcc gtaagcgatg taggccggag cccaggtcgc
     5761 cccggacagt gcacgtccga cgaccaggcg cttgtaggaa ttgatggtcg gcgcgacgat
     5821 cgctgtcagc gcacgcgcgt gcgcgagcac gccgcccgcg aagtgatagg ccatctgcga
     5881 cagccccatg ccgttcttgt ccttgtcgtc gtggaacagg ttcttgacct tgccgttgcc
     5941 aagcgagatg tggaagtgcg cgccggtacc ggtccggttg gagaaaggct tgggcatgaa
     6001 cgtcgcgatc atgcccatcg acttggcgat ctccgaggcc gccatcttga ctaacaccag
     6061 ccggtcggcg gaggccagcg cctcaccgta ggtgaagttg acctcgaact gcgcgttggc
     6121 gtcttcatgg tcgatctggt aaacatcgat gccgaccgcc ctcagcgagt ccaccatcgc
     6181 ttcgaggaac ggtaccgagc gcgccaggcc cttgtagtcg taacagggtt tctccagcag
     6241 gtcggtatcg tccaccggcg ccagcgatcc gtcaccggcg cgcttcagca gcatgaactc
     6301 gggttcgatg ccggtataaa gggtcaggcc ccgcttcgcc aacgcatcga gctggcgctt
     6361 gagcaccacc cgcgaacaat attgatacgg cttgccattc acgtgcccgt agcaagccag
     6421 ccgcgcaaat ccgggcatcc atggaatcag cgatagcgtg gacaggtcgc cgactgccat
     6481 gtagtccggc ccgtgcggcc ccatgccgaa tccccagagc gcgaaaccgg cgaagcccgc
     6541 cccttcgttg agcaccatgt ccaggtggtc caccggcacg gccttcgcct ttgctgcgcc
     6601 gtggatgtcc acgaactgcg cgaggatgta cttgacgtgg tgatccttca ggaatttccg
     6661 tgctgcttgt ggcgacatat ttgcgatctc ccttgtttac gacttcgtca ctccagcttg
     6721 gccgcttgcg acagcaggcg ccccgttcca attggtcctc cgtccagctc tgcggcgatt
     6781 ccggtcaggg tttcccagag atacggcgca taggtgggat cgcaccagat gcggaaaccc
     6841 ggcaggttct ggtgatcctg ccgaatgacc agaaccgcga caccggccat catcgtcatg
     6901 accacggttc gactctctga atcgagttcc tggaaattca cgttgcaagt ctggaccagc
     6961 aactcgttga cgcgcctgcc gaccagcgcg atgccggcgt cctggcgagt caccggatac
     7021 acccccgcga ttcccgctcc gagcgcatcc cgcacccggc ctgccgtatc gccggccgcg
     7081 ccatcctcga gaaagaattc gccgcggccc agccgggcga tcactccgct gcccggcaat
     7141 ccggtccaag cgttcgcctg tgcgggcacg acgatgccca atccctccag ccacggcgcc
     7201 gcttccggcc ccttgacgcc ggctttatcc aggcaactca gatcaacaac cgcgacggat
     7261 tcgcgtgcca cagtttcatc cccggcaaga gcggccgcca tacgcatgcc gcgcacccta
     7321 ccccaccgcg gattcaatgt tgcaacctgg tcgtgcaagg ggctggttcg ctttacgttc
     7381 attgtcaggc ccccaatttc tgccggtcac cggcgggatc gtagaagggt gttttcgcga
     7441 tccgtgccgg caccatctgt ccgccgtcca tgcgaatgaa gaaaccaccg ccgttggcga
     7501 tgatttcggg cgacacgaat gcaaggccga cgtatttctc cattgccggg ctccaggcaa
     7561 tgctggtcac ccggcctgca atttttccat cgcggatgac cagatggcac tccctgggta
     7621 ccttgccttc cgttgccgaa tccagcgtga atccgaccag ctttgctttg gggttcttcc
     7681 gctgcacgat cttcaggctg cgctgcccta cgaagtaggg tttgtccatc ttcacagccc
     7741 aatcgaccgc cgcctcaagc ggcgtggtca gcccgtcggt atcctggccg atgatgatgt
     7801 gtcccttttc caaccgcagc agcctttgcg cttccacgcc gaacgggcgg atgccgtgca
     7861 cgccgccggc ttccatcagc gcatcccata gcgcgcaggc gcgatcggca ggcacgtgga
     7921 tctcgtaacc gagttcgccg acgaagcccg cccgcagcaa ccgtgccggc acaccggcga
     7981 tctcggcttc acgcacgcca aggtagggaa acgcttccgc ggagagatcc agattggtga
     8041 cccccgccaa cggatcgcgc gcgcgcggcc cggcaagatt gaccgcggca tacgcgccgg
     8101 tgacgttgac gatgccgacc tccattcccc acatggtgtt cagtcgcgtc atctcgcgat
     8161 agacgttcgc ggagccggtc gtcgtggtgg tgaagtagaa acgttgctca cccagtcgcg
     8221 ccaccacgcc gtcgtcgatg atcacgccgg catcgtcgag catcagcgcg tagcgggtca
     8281 tgccgacttt catgttggcg aagcgcccgg tgtagatacg ctcgagaaat gtggccgcgt
     8341 ccggtcccga tatttccatt ttgcccagcg tgccgacgtc gatgatgccc accccggtgc
     8401 gaacggccgc cgcctcctcg cgcaccgcct cgagcctggt cttgccgcgc gtcatgtaga
     8461 actcgggacg caaccatatg cccgcctgca tgaataccgc cccggcgcgc tcgtgccgcg
     8521 aatgcaacgg cgtcaggcgg tagggcacga ggccgcggcc tgccaggtgc gacattggca
     8581 ccgggtgaaa gaacggccgc gacgtggtcg tgccaacttc gccgggtgtc gttcccagca
     8641 gcttcgccag gacgcggatc gcgttcatgt tcgaatgctt gccctggctc ggacccatgc
     8701 cgatcgtggc gtagcgcttc agcaactcga tgttgtcgaa gccttcctgc accgcgttgg
     8761 caaggtcctt gagctgaagg tcttcgtcga agtccacgaa gttcttcgcc tgcggatgat
     8821 cgaaaatggg gtacggatgg ctgggcgcga ccgccgcacg cacgaccacc ggtgccgcgg
     8881 atgccggaaa tccgagatac ttcgccgcct cgagcccggc gcgcgacccg tcgcgctggc
     8941 ggtcttcgag cgcgtagacg ccgttgaccc gccccgccgc gaacacgccc ccgggaagac
     9001 tcgcgggcac gaactgcccg acttgtacgt cgaatcgcat tgctgcaccc gcctgatgca
     9061 gcaacgcagc cgccggcgcg tatccgacac tcatcaggac accgtcgcac ctgacggttt
     9121 cgctgcggga ggcatcggcc tcgccgccag tgagcagcgg gcagatgact gccgcgccga
     9181 ccattgaacc actggcgcgc gcttcataaa tgcagtgccc gcgaagggtg cggatgccgg
     9241 cctccgtcac tctcgcctcc tcggccgaac cgccgtcagt gcgacgcagg tccaccaccg
     9301 cctcgacccg gattccgttg gcgatcagat cgagcgccgc cgcatagccg tcctggttgc
     9361 cggtaagcac gaccgcggac tgcatcggcc ttaccgcgta gcgatagatc aggcgctgcg
     9421 ccgccgaggc gagcatgacc ccgggcacgt cgttgttgtg aaacaccgcc ggctgttcgc
     9481 agacaccgct cgcaacgatg attgcgcgcg cgcgaatttt ggtcatgcgc tcggcatcca
     9541 ccagcgccac ccaattgtcg gcgtagcagc cggcagccag cgtgccggtg cgcacgctga
     9601 tgttcggatg cgcgcttata ccagacgcga gattgcgaag ttgcgcagcc ggatcatcgg
     9661 agccgccgcg ctgataagta aggctgccgc caatgcgcgc gttctcgtcc accagcacca
     9721 ctttcgcgcc agcatccgct gccgccagtg acgcgcagag gcccgacgga ccggctccca
     9781 ccaccagtac gtcgcagaag tcgtaacgct tcggcgtccg gatgtgcggt gtggagaagt
     9841 cgagttcgcc caatccggtg acgcgacgga acatgcgttc ccagaacggg aaccacgcct
     9901 tcggggtgtg aaaggccttg tagtagaaac caaccgggag aaccgcggac agcttgtcga
     9961 aaatgcgggc acggtcacgc ctgacgccac cgaaggtatt gacggccgcg agcaccatgc
    10021 gatctcgcag cggttcgacg tcaccgcgca ggttcagtct cgcgccgtcc tggaacatcg
    10081 cgttgacgtc gtggttggcc atcgacagca cgctgcgcgc gcggtggtac ttgaagctgc
    10141 gcccgagcac gcgcacgcct gccgccagca acgcgctcga caccgtgtcg ccggcaaatc
    10201 cctggaaacg ctcgctctcg aaatcgaatc cgaatttctt cgaacgatcg atccattcgc
    10261 cggcgaccaa cggcagccgt gcgctcacgc cggcccctcc ccatacaggt aggttcgcag
    10321 cacctcgtcg gcggcggtat cgcgctcggc gacgaaccag atgccgctgg ccaggtgcag
    10381 ccaccattcc ttcttgatgc ccggcgctcc gttgcggttg aacacataat ccgcccactg
    10441 cgcgtcggtg cacgtatccg catccggcat cggccggatc tcgccggcgt actggaactc
    10501 ggacaccggc cgcaggccgt ttatcgggca acgcatcagc ttcatatgag agaaaacgta
    10561 accacagagg cacagagaga cagagagaga aaaactgatc cactatgggg cggattcatg
    10621 ttgaatgggc aaacccgccg gacagtactc cttgccgcga ctgttccgat tgaatctttc
    10681 atgccgatct ctgcgcctct gtgtctctgt gtctctgagg t
//
LOCUS       DASQOP010000257         3361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_528309, whole genome shotgun sequence.
ACCESSION   DASQOP010000257 DASQOP010000000
VERSION     DASQOP010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528309"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VE030_10200"
     CDS             complement(<1..656)
                     /locus_tag="VE030_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HZF23821.1"
                     /translation="MNDPVDVLIVGAGASGAAVAWSLAETKMHILCLEQGDWMNPANY
                     PSAGRDWEARAYGDYSPSPNIRARPEDYPVNDDNSPIKVVNFNGVGGSTIMYTAHFPR
                     LHPSDFRVRTLDGVAEDWPIDYDTLAPFFAENDRMMGVSGLSGDPAFPPTEPPMPPLP
                     LGRSGARLAKAMNKLGWHWWPSDTTVATVDYEGRARCINLGHCTPACAQGAKASTDIT
                     "
     gene            complement(657..2132)
                     /locus_tag="VE030_10205"
     CDS             complement(657..2132)
                     /locus_tag="VE030_10205"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZF23822.1"
                     /translation="MRDGKAARTRRVGYRGCLSVIALSLVQEQTGNRCNGISSALGAK
                     RIFHPRTQLPLPTLDPVLCCTARQEATARMGSETMAQTEALQHALTSIDVSDPSLYQQ
                     DSWRPLFSQLRRDDPVHYCAASRFGPYWSVTRYDDIFAVELDYENYSSSSELGGIQVT
                     DQPKGRELANFIRMDPPGHTAQRRTVAPIVAPSNLANFEPLIRKRTAGVLDALPRGET
                     FDWVTRVSVELTNMMLATLFDFPWADRERLTWWSDVAIANVDSPDAVVHSEDARFAEL
                     AKMGEYFRKLWDARADAPPAFDLISMLAHAAATRNMPPREFIGTLALLIVGGNDTTRN
                     SMSGGLMALVENPEQFELVRSRHELIPNLVAEIIRYQTPVLHMRRTARNDVELAGRRI
                     AKGDKVVMWYISGNRDEEKIERADEFIVDRPKPRQHLSFGAGIHRCVGDRLAEQQLRI
                     LWEEVLRRNFRFKIMGPPQRLYSNFIRGIRALPVQIIPQGA"
     gene            complement(2072..>3361)
                     /locus_tag="VE030_10210"
     CDS             complement(2072..>3361)
                     /locus_tag="VE030_10210"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZF23823.1"
                     /translation="AMKAVADVGFGDRGILRDTLLLTLAKSQDEKQALGECFDLFFSQ
                     PEVKDEATPADAAEGAPPDSMEGETQPGDPGAQAPELGELAQMLMSRDRNAIAAAMAN
                     AANAASLSDIRYFTQRGIFSSRVLEQLGIERLRDDLDALTAANPAEAERLAAALEALR
                     ENVREVVNQALVLYGREETENLRNEILRNAPLARLERRQVEQMKALIRAIARRLRERY
                     SKPRKRQRRGHLDTRKTLRRNAAWGGVPFLTSWKRRHRDRPKIVAICDVSGSVAQVSD
                     FFLLLIHSLHEVVDDVRSFAFSGHLIEVSDILDTRSPEEAMSEIMSKVGFGSSDYGGS
                     LVDFEKGWIRTVTAKTTVIVLGDARTNNLDPRADILRRISERSKRLVWLNPEGRMAWG
                     WGDSEMPRYAAFCSVVRQCATAKQLERAVSDIVAAYQ"
BASE COUNT          626 a         1056 c         1051 g          628 t
ORIGIN      
        1 taggtgatgt cagtgctcgc cttcgccccc tgcgcgcagg ccggcgtgca atgacccaga
       61 ttgatgcatc gcgcgcgccc ctcgtagtcg acggttgcca ccgtcgtgtc ggagggccac
      121 caatgccagc cgagcttgtt catggccttc gcaagacggg cgccggagcg gccaagcggc
      181 agcggcggca tcggaggttc cgtcggcgga aaggcagggt cgcccgatag cccggagaca
      241 cccatcatcc ggtcgttctc ggcaaagaac ggcgcgagcg tgtcatagtc aatcggccag
      301 tcctcggcca cgccatccag cgtgcgcacg cggaaatccg aagggtgaag ccgcggaaaa
      361 tgcgccgtat acatgatggt agagccgcca acgccgttga agttcacaac cttgatcggc
      421 gagttgtcgt cattaacggg ataatcctcg ggccgggcgc gaatgttggg gctcggtgag
      481 tagtcgccat aggctcgcgc ctcccagtcc cgccccgcgc tcggataatt ggcaggattc
      541 atccagtcgc cttgttcgag gcacaggatg tgcattttgg tctcggcaag tgaccacgcc
      601 acggcagcgc cggacgctcc agcgccaacg atcaagacat ccacggggtc gttcattcaa
      661 gcgccttgcg gaatgatttg caccgggagg gcgcggatgc cgcggatgaa gttcgaatag
      721 agccgctgtg gcggtcccat gatcttgaag cgaaaattcc gccgcaggac ctcctcccag
      781 agaatccgaa gttgctgctc ggcgagccga tcgccgacac agcgatggat gccggcgcca
      841 aacgacagat gctgccgcgg cttcgggcga tcgacgatga attcatcggc gcgttcaatt
      901 ttttcttcgt cacgatttcc ggagatgtac cacatcacca ccttgtcgcc tttggcaatc
      961 ctgcggccgg cgagctcgac atcgttgcgc gcggtgcggc gcatgtgcag caccggcgtc
     1021 tgataacgaa tgatttcggc gaccaggttc gggatcaact catgccggct gcgcacaagc
     1081 tcgaactgtt ccggattttc gaccagcgcc atcagtccac cgctcatgga attgcgcgtg
     1141 gtgtcgttac cgcccacgat cagaagcgca agcgtgccga taaattcgcg cggcggcatg
     1201 ttgcgggtcg cggcggcatg ggcgagcatc gaaatcaggt cgaaggccgg cggcgcgtcc
     1261 gcgcgcgcgt cccacaattt acggaaatat tctcccattt tggccagctc ggcgaaacgc
     1321 gcatcctcgg aatgcacgac ggcatcgggc gaatccacat tggcgatggc gacatcggac
     1381 caccaggtca gcctctcccg gtccgcccag ggaaaatcga acagcgtcgc cagcatcatg
     1441 ttggtcagtt cgaccgaaac gcgcgtcacc cagtcgaagg tctcgccacg cggcagcgca
     1501 tcaagcacgc cggcggtacg cttgcggatc agcggctcga aattggcgag attggacggc
     1561 gccacgatcg gcgccacggt acggcgctgc gcggtgtgcc cgggcggatc cattcgaatg
     1621 aaattcgcaa gctcccgccc cttcggctga tcggtaactt ggatgccgcc gagttccgaa
     1681 ctcgaggaat agttttcata gtcaagctca acggcgaaga tgtcgtcgta gcgcgtcacc
     1741 gaccagtagg gtccgaaccg cgacgccgcg cagtaatgga cgggatcatc gcggcgcaac
     1801 tgcgaaaaca gcggccgcca ggagtcttgc tgataaaggc tgggatcgct gacgtcgatc
     1861 gacgtcagcg cgtgttgcaa cgcttctgtc tgggccatcg tttcgctccc catccgtgcc
     1921 gttgcctctt gtcgggcggt gcagcatagg acgggatcga gcgttggcaa ggggagctga
     1981 gttcgcgggt ggaatatccg ctttgcacca agagcggatg aaatcccatt gcaccggttg
     2041 ccggtctgtt cttgaaccaa gctcaatgca attactgata agcagccacg atatccgaca
     2101 cggcgcgttc gagctgcttt gccgtcgcgc actggcgcac gacgctgcag aatgcggcat
     2161 atcgtggcat ttcggaatcg ccccagcccc aggccatgcg tccttcgggg ttgagccaga
     2221 ccagccgctt ggatcgctcc gaaatgcggc gaaggatgtc ggcgcgcgga tcgaggttgt
     2281 tggtacgcgc gtcgccgaga acgatcaccg tcgtcttcgc ggttaccgtc cggatccaac
     2341 ccttctcgaa gtccaccaat gaaccgccgt agtcggacga gccgaatccg accttcgaca
     2401 tgatctcgct catggcctct tccggcgacc tggtatcgag gatgtcgctg acctcgatga
     2461 gatggccgga gaaggcgaac gagcgcacgt cgtccaccac ctcatgcagg ctgtggatca
     2521 acagcaggaa gaagtcggat acctgcgcca ccgatcccga gacatcgcag atcgccacga
     2581 tcttcggccg gtcgcggtgg cggcgtttcc atgaggtgag gaacggaacg ccgccccagg
     2641 cggcgttgcg ccgcagcgtc tttcgcgtgt cgagatgccc gcgccgctgg cgcttgcgcg
     2701 gcttgctgta acgctcacgc agccgccgcg cgatggcgcg gatcagggcc ttcatctgct
     2761 cgacctgccg gcgttcgagc cgggcgagcg gcgcgttgcg gaggatttcg ttacgcaggt
     2821 tctcggtctc ctcgcggccg tagagtacca gcgcctggtt gacgacctcc cgcacgtttt
     2881 cgcgcaacgc ctccagggcg gcagccaacc gttcagcctc cgccggattg gcggccgtca
     2941 gcgcgtccag atcgtcgcga aggcgttcaa tgccgagttg ctcgagaacc cgtgacgaga
     3001 agatgccgcg ctgggtaaaa taccggatgt cggacagcga ggcggcattc gccgcattgg
     3061 ccattgcggc cgcgatcgcg ttgcggtccc gcgacatcag catctgcgca agctcgccaa
     3121 gttccggtgc ttgtgcgccg gggtcgcctg gctgggtttc gccttccatc gaatctggcg
     3181 gagcgccttc ggcagcatcc gccggcgtgg cttcgtcctt cacttccggc tggctgaaga
     3241 aaagatcaaa acactcgcca agcgcctgct tctcgtcctg gctcttggcg agcgtcaaca
     3301 gcagtgtgtc acgcagaata cccctgtcgc caaatccgac atcggccacc gctttcatgg
     3361 c
//
LOCUS       DASQOP010000258         2877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1537169, whole genome shotgun sequence.
ACCESSION   DASQOP010000258 DASQOP010000000
VERSION     DASQOP010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1537169"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="VE030_10215"
     CDS             complement(<1..989)
                     /locus_tag="VE030_10215"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HZF23824.1"
                     /translation="MASAVAPAGGRTAALSSQVGAWRNALAEGRETLKADFLERPDPA
                     KALDRQRMLVDEVLKTIWSETGQADELALIAVGGYGRGTLYPYSDVDVLVLLPDQASD
                     AARARVEELVGVLWDIGLEIGHSVRTIAGCSEEASRDVTVQTTLLESRLVAGNRKLYS
                     SFVKSIEEGLDVPYFVEAKLLEQQQRHTRFNDTAYNLEPNLKESPGGLRDLQNILWIA
                     RAARLGRSWGDLTKQRILTREEARQLLRHGSTLAELRVRLHYLASRREDRLLFDYQSA
                     LAQEYGLSDTPARRASEQLMQRFYSTAKQVQLLNTIVLQNLRGRIFPSVDAEP"
     gene            complement(990..1793)
                     /gene="map"
                     /locus_tag="VE030_10220"
     CDS             complement(990..1793)
                     /gene="map"
                     /locus_tag="VE030_10220"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HZF23825.1"
                     /translation="MMSVTIKTPEEIEKMRVAGRLAAEVLDFITPYVRPNVTTDELDA
                     LCHDYMVNVQKTVPAPLNYAPPGYQPYPKSICTSVNHVVCHGVPGPKKLKAGDVINVD
                     VTVIKAGFHGDTSRMFFVGEPSIQARRLSEITYECMWRGINAVKPGAFLGDIGYLIQS
                     HAEEHGFSVVREFCGHGIGRKFHEEPQVLHYGRAGTGLKLQPGMIFTVEPMINAGKPA
                     IRQLADGWTVVTKDHSLSAQWEHTVLVTDSGFEVLTLSDGAPAPPSFVA"
     gene            complement(1947..2022)
                     /locus_tag="VE030_10225"
     tRNA            complement(1947..2022)
                     /locus_tag="VE030_10225"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1987..1989),aa:Asn,seq:gtt)
     gene            2091..>2877
                     /locus_tag="VE030_10230"
     CDS             2091..>2877
                     /locus_tag="VE030_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23826.1"
                     /translation="MKSRKRWYLSTGIAFALLLVVPFVLPLGGFIPEIERVASEQLKA
                     PVKIESLRLFFLPLPRLSVEGISVGRKPFLQVQKVQVTPRLTSLFSEQKVIREISLRG
                     VVMNQQLINKASTWASQPGSGGPAPVRVERIEIRDANINFTDFKLHQAEVDLELTLDG
                     GLAQAQVRADHGHVAATLVPRGKDFALDLSAQNWKLPAGPPIVLSSLAALGIVNADGL
                     VLSTIDGRLYDGTLAGRLTVGWKKEWTIAGNLDVREVEIGPVVA"
BASE COUNT          505 a          860 c          945 g          567 t
ORIGIN      
        1 ctgggttcgg catccaccga ggggaaaatg cggccccgca ggttctgcag cacgatggtg
       61 ttcagcagct gcacctgctt cgcggtgctg tagaagcgct gcatcaactg ctcgctggcg
      121 cgtctggccg gcgtatccga aagcccgtac tcctgcgcga gcgcggactg ataatcgaac
      181 agcaggcggt cttcccgccg cgaggccagg taatgcagcc gcacccgcaa ttcagcaagt
      241 gtggacccgt ggcgtagcag ttgccgcgct tcttcgcgcg ttaggatgcg ctgtttggtg
      301 agatcgcccc aactgcgccc cagccttgcg gcacgcgcga tccacaggat gttctgcaga
      361 tcgcgcaggc cgccagggct ttccttgagg ttcggctcga ggttgtaggc cgtgtcattg
      421 aagcgggtat ggcgctgttg ctgctcgagg agtttggctt cgacgaaata ggggacgtcc
      481 agtccttcct cgatggactt gacgaagctc gaatagagct tgcggttgcc ggcgacgagc
      541 cgcgattcca gcagcgtggt ttgcacggtg acatcgcggc tcgcctcttc gctgcagccg
      601 gcgatggtgc ggacgctgtg gccgatctcc aggccgatgt cccagagcac gccaaccagt
      661 tcttcgacgc gcgcgcgcgc cgcgtcgctc gcctgatcgg gcagcagcac cagcacgtcg
      721 acgtcggagt aggggtagag cgtgccgcgg ccgtatccac ccacggcaat cagcgccaat
      781 tcatccgcct ggccggtttc ggaccagatc gtcttcagca cctcgtcgac cagcatgcgc
      841 tggcgatcca gcgccttggc gggatcgggc ctttccagga agtcggcctt gagcgtctcg
      901 cgcccctctg cgagggcatt gcgccacgcg ccaacctggg atgacagcgc ggccgtgcgt
      961 ccgccggctg gagcgaccgc cgaagccatt tacgcgacga aggacggcgg cgccggcgcg
     1021 ccgtcggaca atgtcagcac ttcgaatccg ctatcagtga ccagcaccgt gtgctcccac
     1081 tgtgccgaca gcgagtggtc cttggtgacc acggtccagc cgtcggcgag ctggcggatg
     1141 gcgggcttgc cggcgttgat catcggttcg acggtgaaga tcattcccgg ttgcagcttc
     1201 agtcccgtgc cggcacggcc gtaatgcagg acctgcggtt cttcgtggaa cttcctgccg
     1261 attccatggc cgcagaactc acgcactacc gagaagccgt gctcttccgc atgcgactgg
     1321 atcagataac cgatgtcgcc gaggaaagcg ccgggcttca ccgcgttgat gccgcgccac
     1381 atgcattcgt aggtgatctc ggaaagccgg cgcgcctgga tcgacggctc gcccacgaag
     1441 aacatgcggc tggtgtcgcc gtgaaagccg gccttgatga cggtgacgtc gacgttgatg
     1501 acgtctccgg ctttgagttt tttcgggccg ggtacgccgt ggcagaccac gtggttcacc
     1561 gatgtgcaga tcgacttggg gtagggctga tagcctggcg gtgcgtaatt tagcggggca
     1621 ggcacggttt tctgcacgtt gaccatgtag tcatggcaca gcgcatcgag ttcgtcggtg
     1681 gtgacgttgg gcctgacata cggcgtgatg aaatcgagca cctccgcagc gagacggccg
     1741 gcgacgcgca tcttctcgat ttcttccggg gtcttgatgg tgacggacat gattgaagtg
     1801 gcgattttcg cgcggcaatg ataacacccg ggatcgccgg ttcggcgcgc atgcgatgag
     1861 acagaatctg gggccggcgc gataacaaat caagctgcgc ggcgatggtg aggcgcttgt
     1921 gctggaggga aacgggacgc gggagttggc tccccgacca gggctcgaac ctgggacctg
     1981 cggattaaca gtccgtcgct ctaccaactg agctatcggg gaagataaag gccgcgtatc
     2041 atatccgctc gctccaacac ggtcaacgga ttccccgtcc gtcagttcga atgaaatcca
     2101 ggaagcgctg gtatctcagt accggcattg cctttgcact tctgctcgtc gttcctttcg
     2161 tcctgcccct gggaggattc attcccgaaa tcgagcgcgt ggcttcggag cagctcaagg
     2221 cgccggtcaa aatcgagtcg ttgcgattgt ttttcctgcc gttgcctcgc ttgtccgtcg
     2281 aaggcatctc ggtagggcgg aagcccttcc tgcaggtgca aaaagtccag gtcacgccgc
     2341 ggctgacttc cctgttcagc gagcagaaag tgattcgtga gattagcttg cgtggcgtcg
     2401 tgatgaacca gcagttgatc aacaaggcct cgacatgggc gagccagccg ggttccggcg
     2461 gaccggcgcc ggtgcgtgtg gaacgcatcg aaatccgcga cgccaacatc aatttcaccg
     2521 atttcaagct gcaccaggcc gaggtcgatc tggaacttac gctcgacggc gggttggcgc
     2581 aggcacaggt gcgtgccgac catggacatg tggccgcgac tctcgtgccg cgagggaagg
     2641 atttcgcgct cgatctttcg gcgcagaact ggaagttgcc ggccgggccg ccgattgtgc
     2701 tcagcagcct ggctgcgttg ggcatcgtca acgcggatgg actggtgctg tccaccatcg
     2761 acggccgtct ctacgacggc acgcttgccg gcaggctgac ggtgggctgg aaaaaggaat
     2821 ggaccatcgc cgggaatctc gacgtccggg aggtggagat cgggccggta gtcgcgc
//
LOCUS       DASQOP010000259         4057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_126738, whole genome shotgun sequence.
ACCESSION   DASQOP010000259 DASQOP010000000
VERSION     DASQOP010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126738"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..1233)
                     /locus_tag="VE030_10235"
     CDS             complement(22..1233)
                     /locus_tag="VE030_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZF23827.1"
                     /translation="MNPPVNWRTPVLVLVCGAIILTLGLGIRASFGLFLQPMSTDLGW
                     GRSHFSFAMALSNLIWGLAQPFFGAWADKKGAGRVVAVSGLLYAGGLALMPFSTTPLM
                     LDASAGLMVGLGLSGVSFGVILGVVGRAFPPERRSLALGIASSGGSFGQFLMLPFGQL
                     LISTLGWQTALLVLAGIILATVPLAAAMMEGRRPAATGAQQSLGEALREAGAHSGFWY
                     LTAGFFVCGFQVAFISVHLPAYLLDVKMTPAVGATALALIGLFNIAGSFVAGYLGGRF
                     SKKYLLSGIYVLRAVIIALFLVLPVSPLTVYLFAAGIGFLWLGTVPLTNGLVAQIFGV
                     RFVSMLFGIVFLSHQIGSFTGVWLGGYLFDATGSYDLVWIGSIALGVIAAILNLPIDE
                     RPVVRAEARAA"
     gene            complement(1421..1807)
                     /locus_tag="VE030_10240"
     CDS             complement(1421..1807)
                     /locus_tag="VE030_10240"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HZF23828.1"
                     /translation="MNRILNIGVAQKIGTYSDGVSVPADAKWVFVSGTPGMLPDGSCP
                     KGITAQAEQAWKNVLAILAEAGMGVKDLVKVTHYLVRQEDIKDYVVVRARYLGDARPA
                     SMLLVVPGLVKPEILVEIEAYAARQG"
     gene            complement(1955..>4057)
                     /locus_tag="VE030_10245"
     CDS             complement(1955..>4057)
                     /locus_tag="VE030_10245"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZF23829.1"
                     /translation="VLVIFLFLRSWRATLIPAVAIPVSVIGHFTFLKTMGFSINTLTL
                     LGVVLSIGLVVDDAIVVLENIHRHIEEGMKPIKAAFQGSKEIGFAVIAMTITLAAVFT
                     PLALMTGNTGKLFTEFALTVATSVIVSGFVALTLTPMMCSRLLKPHGAHSTIYNVTEH
                     FFEGMNNGYRAILRATLRHRWVIGVLFVVVLGAMVLVYKQLKSELSPVEDRGLFLAFV
                     ITPEGSTMQYTDGYMHEVEAITKSIPEITTMFAVVAPGLDRPNPVNLGVAFAVLQPWS
                     ERHRTQMEITKELGPKLFGGLPGALSFALNPPSLGQGFLTKQVEYVIYGNSYEELQTA
                     VGKVMGKLREYPGMIALDTDLKLNKPQLAVEINREKAADLGVSMEVIGHTLQTLLGSR
                     DVTRYKHEGKQYDVVVQMEDNKRMQPTDLTSIYVRGRDDQLIQLSNLVRLNEAVAPKE
                     LNHFNKLRAAVINGNVGPGASLGQVLDTIDATVKAELPSGTITDLDGQAREFREAGGQ
                     LFLTFLLALIFIYLVLSGQFESFVGPLVIMFTVPLALTGALLVMWINSMIGNGGTLNV
                     YSQIGLVMLVGLITKHGILIVEFANQLRSRGMEKFEAVVEAATLRLRPILMTTGAMIL
                     GAIPLALAHGAGAESRRAIGWVVVGGLAFGTFLTLFVIPTIYTVMMRRVHMADEDEVA
                     LPVTAGGGGAITAPHREL"
BASE COUNT          858 a         1306 c         1183 g          710 t
ORIGIN      
        1 cagcgcgcca acccggaacg atcacgcggc gcgcgcctcc gcgcgcacga ccggccgctc
       61 gtcgatggga agattgagaa tcgccgcgat cacgcccaag gctatgctgc cgatccacac
      121 cagatcgtac gagccggtgg catcgaacag gtagccgccc agccacacgc cggtgaaact
      181 gccgatctga tggctgagga agacgatgcc gaacagcatg gacacgaagc gcacgccgaa
      241 gatttgcgcg accaggccgt tggtgagcgg caccgtgccc agccacagga atccgattcc
      301 ggccgcgaac agatagaccg tcagcggcga taccggcaat accaggaaca gcgcgatgat
      361 cacggcacga agaacataga tgccggagag caggtacttc ttgctgaagc gcccgccgag
      421 ataaccggcg acaaacgagc cggcgatgtt gaacagcccg atcagcgcca gagcagtcgc
      481 gcccacggcg ggcgtcattt ttacgtccag caggtacgcc ggcaggtgca ccgagatgaa
      541 agccacctga aagccgcaca cgaagaagcc cgcggtcagg taccagaagc cggaatgagc
      601 accggcttcg cgcagcgctt cacccagcga ttgctgtgcg ccggtggcgg ctggacgccg
      661 cccctccatc atcgccgcgg ccagcggaac cgtcgccagg ataatcccgg ccaacaccag
      721 caacgcggtc tgccagccca gggtggaaat caggagctgc ccgaacggca gcatcaggaa
      781 ctgcccgaaa gatccgccgg aagacgcgat gccgagtgcg aggctgcggc gctcgggcgg
      841 aaacgcgcgg cccaccacgc cgaggatgac gccgaaactc actccgctca gccccaggcc
      901 caccatcagt ccggcgctcg catcgagcat cagcggcgtc gtggaaaacg gcatcagcgc
      961 caggccgccg gcatacagga gtccggaaac cgcaaccacg cgcccggcac ccttcttgtc
     1021 tgcccaggca ccgaagaacg gctgcgccag cccccagatc agattcgaca gcgccatcgc
     1081 gaaggagaag tggctgcgcc cccagccgag atccgtgctc atcggctgca ggaacaatcc
     1141 gaagctcgca cggatgccca atcccagcgt gaggatgatc gcgccgcaga ccagtacgag
     1201 gacgggggta cgccagttca cagggggatt catgatgggg gacccgggtc aaaaggcgag
     1261 acaggcatac cgggaattca ttgcaaattg cattctccgt gcctcggatg tggacacaag
     1321 cgacatttca gaatgcttcg catgcatttc gggaatggca aggatatcga aaaaggcgtg
     1381 ccactcggca caccgcgctt catccttcat ccttcctcct ttatccttgc cttgctgcgt
     1441 aggcttcgat ctcgacgagt atttccggct ttaccagccc cgggacgacc agcagcatcg
     1501 aggccggccg cgcatcgccg agatagcggg cgcgcaccac gacgtagtcc ttgatgtctt
     1561 cctgtcgcac cagatagtgc gtgaccttga cgaggtcctt cacgcccatc cctgcttccg
     1621 caaggattgc caacacgttc ttccaagcct gctcggcctg cgcggtgatg cccttcgggc
     1681 aggatccgtc cggcaacatt cccggcgtac ccgagacgaa cacccatttc gcatccgcgg
     1741 gcaccgacac gccatcgctg taagtgccga ttttctgcgc cacgccgata ttgagaatgc
     1801 ggttcatgtt cgtctcgccc ttcacccttc acccttcatc cttccgcctt gctcttcatc
     1861 cttcacgttt gtcgccatcg actatagcga aaaaaaacgg caagtgcagg agcacttgcc
     1921 gcttcgaatc aaacgattgt gtaccggtcc ggaatcagag ttccctgtgg ggcgcggtga
     1981 tcgcaccgcc accgcctgcc gtcacgggga gcgcgacttc gtcctcgtcg gccatgtgca
     2041 ccctgcgcat catcaccgtg tagatggtcg gaatgacgaa cagcgtcagg aatgtgccga
     2101 aggccagtcc gccgacgact acccagccga tggcacggcg cgattcggca cccgctccgt
     2161 gcgcgagcgc cagcggaatc gcgccgagaa tcatcgcgcc tgtcgtcatc aggattggcc
     2221 gcaggcgcaa cgttgccgcc tccaccaccg cctcgaattt ctccatgccc cggctgcgca
     2281 gttgattggc aaattccacg atcagaatgc cgtgcttggt aatcaggccc accagcatca
     2341 ccaggccgat ctggctgtag acgttcagcg tgccgccgtt gccgatcatg gaattgatcc
     2401 acatcacgag cagcgcgccg gtcaacgcca gcggcaccgt gaacatgatc accagcggac
     2461 cgacgaagct ctcgaactgt ccggacagca ccaggtagat gaagatcagg gccaacagga
     2521 aggtgaggaa caactgtccg ccggcttcac ggaactcgcg agcctgccca tccagatccg
     2581 tgatggttcc ggacggcagc tccgccttga ctgtagcatc gatggtatcg agcacctggc
     2641 ccagagaggc gccgggtccg acgttgccgt tgattaccgc agcgcgcagc ttgttgaagt
     2701 ggttgagttc cttcggcgcg accgcctcgt tgagcctcac cagattggaa agctgaatca
     2761 actgatcgtc cctgccgcgt acgtatatcg aagtcagatc ggtaggctgc atgcgtttgt
     2821 tgtcttccat ctgcaccacg acgtcgtatt gcttgccctc gtgtttgtag cgggtaacgt
     2881 cgcggctgcc gagcagtgtt tgcaatgtgt gaccgatgac ttccatcgaa actccaagat
     2941 cagccgcctt ttcgcgattg atctccacgg ccagctgggg cttgttgagc ttcagatcgg
     3001 tgtcgagggc aatcatgccg ggatactcgc gcagcttgcc catgaccttg ccgaccgcgg
     3061 tctgcagttc ctcgtagctg ttcccgtaaa tcacatactc gacctgtttg gtcaggaatc
     3121 cctggccgag cgacggcggg ttgagcgcga acgagagtgc gccaggcaag cccccgaaca
     3181 gtttcggccc cagttccttg gtgatctcca tctgggtgcg gtggcgttca ctccagggtt
     3241 gcagaactgc gaaagcgacg ccgaggttga ccgggttcgg acggtcgagc ccgggagcga
     3301 ccaccgcgaa catcgtggtg atttccggga tcgacttggt gatggcctcg acctcgtgca
     3361 tgtagccgtc ggtgtattgc atggtagagc cttcgggcgt gatcacgaag gccaggaaca
     3421 ggccgcggtc ttccaccggg gagagttccg acttcaactg tttgtacacc agcaccatgg
     3481 cgcccagcac cacaacgaaa agaacgccga tgacccagcg atgccgcagc gtcgctcgca
     3541 ggatcgcccg gtaaccattg ttcatgccct cgaaaaagtg ctcggtcacg ttgtagatgg
     3601 tgctgtgcgc gccatgcggc ttgagcagcc tggaacacat catcggcgtc agcgtcagcg
     3661 cgacaaaacc ggaaacaatc accgacgtcg ccaccgtcag cgcgaactcg gtgaacaact
     3721 tgccggtatt gccggtcatc agcgccagcg gcgtgaacac ggccgcaagc gtgatggtca
     3781 tggcaatgac cgcaaaaccg atttccttgc tcccctggaa ggcggcttta atcggcttca
     3841 tgccttcctc gatgtggcga tggatgtttt ccagcaccac gatggcgtcg tccaccacca
     3901 gcccgatcga caacaccacg cccaagagcg tcagcgtgtt gatcgaaaaa cccatcgtct
     3961 tgaggaaggt gaagtggccg atcaccgaaa ccgggattgc cacggcagga atcagggtcg
     4021 cccgccaact gcgcaagaac aggaagatca ccagcac
//
LOCUS       DASQOP010000260         2599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2944173, whole genome shotgun sequence.
ACCESSION   DASQOP010000260 DASQOP010000000
VERSION     DASQOP010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2944173"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..363
                     /gene="grxC"
                     /locus_tag="VE030_10250"
     CDS             76..363
                     /gene="grxC"
                     /locus_tag="VE030_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010394169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HZF23830.1"
                     /translation="MPRISMYCTAWCPYCIAAERLLTAKGAVEIEKIRVDLEPARRAE
                     MMQRSGRRTVPQIYFDDFHVGGFDDLAALDQAGKLEPMLDRERTGARSHAG"
     gene            389..847
                     /gene="secB"
                     /locus_tag="VE030_10255"
     CDS             389..847
                     /gene="secB"
                     /locus_tag="VE030_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HZF23831.1"
                     /translation="MSQQPTQPVFSIEKLFVKDLSLEVPGAPRIYLEREAPQVEIQMN
                     NSASPIDEGYFEVVLTVTVTAKIGDKTVFLVEVSQGGVFQIRNVPQAELEPVLGITCP
                     NILYPYVREVVSEAVVRAGFPPVVLSPVNFEAIFQARQQGQTQAPEGATH"
     gene            860..1312
                     /locus_tag="VE030_10260"
     CDS             860..1312
                     /locus_tag="VE030_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HZF23832.1"
                     /translation="MKTPAALLRCGFLAGLLLCSGAAVALEFRSVAEAGAILYDAPSA
                     KAQKLFVLSRDYPVEVVVKVEGWTKVRDDTGEFAWIENRQLSERRTVLVKSSGAEARQ
                     SASDAAALVFTAEKGVVLEFLQLAAGWVKVRHPDGAVGFIKVSQLWGV"
     gene            1418..2410
                     /locus_tag="VE030_10265"
     CDS             1418..2410
                     /locus_tag="VE030_10265"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008334202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HZF23833.1"
                     /translation="MKIAILGAGAWGTALGIALSARHEITLWCRDPQQSGLLAAERVN
                     VRYLPGVAIPGAVRIGADADAALQSCELVIVAVPAAALRETARCVAHQRRALDVVWLC
                     KGFESGSAKFPHQFVGEELGARFRFGALSGPSFADEVARGLPAAVTLASRDAEFAAAA
                     ARELHTGRLRVYSSDDLAGVETGGAVKNVIAIAAGVCDGLQLGSSARAALITRGLAEM
                     TRLGTRLGGRLETFMGLAGVGDLVLTATSDLSRNRRVGLGLASGRRLADILGNLGHVA
                     EGVFSAREVLRLAQDLDIDMPITRAVCRVLDGHASARDAVEELLQREPKPESMR"
     gene            complement(2407..>2599)
                     /locus_tag="VE030_10270"
     CDS             complement(2407..>2599)
                     /locus_tag="VE030_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HZF23834.1"
                     /translation="GDVLVFGPETSGLPEGLRASFEPRRQLRLPMVPVSRSLNLSNAV
                     AAVVYEAWRQNGFMGASSS"
BASE COUNT          460 a          808 c          863 g          468 t
ORIGIN      
        1 ggcttcggtg aggtggtggt gttgtcaggc ggcttcggag cctggcaaca ggccaatctt
       61 ccggtggaga aatagatgcc gcggatcagc atgtactgca cggcctggtg tccctactgc
      121 attgccgcgg aacggctgct caccgccaaa ggcgcggtgg aaatcgaaaa aatccgcgtc
      181 gacctggagc cggcgcggcg cgcggaaatg atgcagcgga gcgggcgccg caccgtaccg
      241 cagatctact tcgacgactt ccacgtcggg ggcttcgacg atctggccgc actggaccag
      301 gccggaaagc tcgagcctat gctcgacaga gagcgcaccg gcgcacgcag ccacgccggc
      361 tgacccgtca accaagagaa caagaatcat gagtcaacaa cctacccagc cggtcttttc
      421 aatcgagaag ctgttcgtca aggatctgtc gctggaagtg cccggcgcgc cgcgcatata
      481 tctggagcgg gaggcaccgc aggtcgagat ccagatgaac aacagcgcca gcccgataga
      541 tgagggctac ttcgaggtcg tgttgacggt gaccgtgacc gccaagatcg gcgacaagac
      601 cgtgttcctg gtggaggtga gtcagggcgg ggtgtttcag atccgcaacg tgccgcaggc
      661 ggagctcgag ccggtgctcg gcatcacctg cccgaacatc ctttacccct atgtgcgcga
      721 agtagtttcc gaggcagtgg tgcgcgccgg cttcccgccg gtggtgttga gcccggtgaa
      781 tttcgaagcg atcttccagg cccggcaaca aggccagacg caggcgcccg agggcgccac
      841 gcactagccc ggataattca tgaagacgcc cgctgcgctg ctgcgatgcg gttttctggc
      901 ggggctgttg ctctgttccg gggctgcggt tgcgctggaa ttccgctcgg tggcggaggc
      961 gggggcaatc ctttacgacg cgccgtcggc caaggcacaa aagctcttcg tgctgtcgcg
     1021 cgattacccg gtggaggtgg tggtcaaggt ggagggatgg accaaggtgc gcgacgacac
     1081 cggagaattt gcctggatcg agaaccgcca gttgtccgag cgccgtaccg tgctggtcaa
     1141 gtcctcgggc gcggaagcgc gccagtccgc cagcgatgca gccgcgctgg tgttcaccgc
     1201 cgaaaaaggc gtggtgctcg agtttctcca actcgccgcc ggctgggtca aggtacgcca
     1261 ccccgacggc gcggtcggtt tcataaaggt cagccagctc tggggcgtat gacacagtgt
     1321 tgagttttaa gttttgagtt ttaagttagt gtcagccagc cgcgcttaac tcaacactca
     1381 acactcaaaa ctcaacactg aaaattcacc actccgcatg aagattgcga tattaggtgc
     1441 cggcgcatgg ggcacggcgc tcgggatcgc gctttcggcg cgccacgaaa tcacgctgtg
     1501 gtgccgcgat ccgcaacaga gcggcctgct tgccgccgaa cgcgtcaacg tccgctatct
     1561 tccgggtgtg gccattcccg gcgcggttcg gatcggcgcc gatgccgacg ccgcactgca
     1621 gtcctgcgaa ctggtcatcg ttgctgttcc cgctgccgca ctgcgcgaaa cggcgcgatg
     1681 cgtcgcgcac cagcgacgcg cgctggatgt ggtctggcta tgcaagggtt ttgagagtgg
     1741 cagcgccaaa tttccgcacc agttcgtcgg cgaggaactg ggcgcgcgct tccgcttcgg
     1801 cgcgctgtcg ggaccgagtt tcgccgacga agtggcgcgc gggctgccgg cggcggtcac
     1861 gctggcttcg cgcgatgccg aatttgccgc cgccgccgcg cgcgagttgc ataccgggcg
     1921 cttgcgcgtg tactccagcg atgatcttgc cggcgtcgag accggcggtg cagtcaagaa
     1981 tgtgatagcc attgccgcgg gggtttgcga cggcctccaa ctcggctcca gcgcacgcgc
     2041 ggcgctgatt acccgcgggc tggcggaaat gacccgcctc ggcacgcgcc tcggcggacg
     2101 gctggaaacc ttcatgggat tggcaggtgt cggcgacctg gtgttgacgg cgacctcgga
     2161 cctgtcgcgg aaccggcggg tcggcctggg gctcgcaagc ggacggcgcc tggcggatat
     2221 cctgggcaat ctggggcacg tcgccgaagg cgttttcagc gcccgcgagg tgctgcggct
     2281 ggcgcaggac ctggatatcg atatgcccat cacccgcgcg gtctgccgcg ttctggacgg
     2341 ccacgcctct gcccgcgatg cggtggaaga gctgcttcag cgtgaaccca agcccgaaag
     2401 tatgcgctaa ctgctgctgg cacccatgaa gccgttctga cgccacgctt catagaccac
     2461 agccgcgacc gcattggaca gattcagact gcggctgacc ggcaccatcg gcagcctcag
     2521 ctgtcgtcgc ggctcgaagc ttgctcttag cccttccggt aatccgctcg tctccggacc
     2581 gaaaaccagc acgtccccc
//
LOCUS       DASQOP010000261         8613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1136264, whole genome shotgun sequence.
ACCESSION   DASQOP010000261 DASQOP010000000
VERSION     DASQOP010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8613
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136264"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2114)
                     /locus_tag="VE030_10275"
     CDS             complement(<1..2114)
                     /locus_tag="VE030_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HZF23835.1"
                     /translation="MEKSFKSEIELLRLGNGQVFHGEGILAITKALLQSGVAYVGGYQ
                     GAPVSHLIDVMIQAEDYMGELGVHVEACSNEASAAAMLGASIHYPLRGAVAWKSIVGT
                     NVAADALSNLASPGVIGGALIVIGEDYGEGASVIQERTHAYALKSSMLMLDPRPELSN
                     MVRMVEDGFRLSEASNMPALMELRIRACHVRGSFVCKDNVAPAVSTRHLMEEPAKFDY
                     TRLAHPPVTFRHEKLKGEARIPAARRYIAEHGLNELFAGRRDDVGLIVQGGLHNSLVR
                     ALQQFGMADAFGECEIPIFVLNVTFPLVPEEISKFCLGKRAVLIVEEGQPEYIEQEIL
                     ATLRRLDVNTPIHGKDMLMAAGEYTVEVMAGGLEKFAERYLPAGVAGTGKRWLQGNAQ
                     RRQEVVQQFSAPLPARPPQFCIGCPERPVFAALKLSQRDVGPTHIAADIGCHSFSTFE
                     PFSFGHSILGYGMSLASRAGVSPMMKRRVLSIMGDGGFWHNGLLTGVQSALFNGDDAV
                     LLIFKNGYTAATGTQELISTPDEVVKITAENKQQSLLHRNLTIEKTLSGIGVKWMRTV
                     HTYHVERMRKTLDEAFTTDYSGLKVIIAEGECQLERQRRIKPWVAGLLKAEKRVVRVK
                     YGVDEDVCTGDHACIRLSGCPTLTLKDSPDPLRVDPVATVIDGCVGCGLCGENAHAAT
                     LCPSFYRAEVIRNPRLDERIIDG"
     gene            complement(2176..6099)
                     /gene="hrpA"
                     /locus_tag="VE030_10280"
     CDS             complement(2176..6099)
                     /gene="hrpA"
                     /locus_tag="VE030_10280"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HZF23836.1"
                     /translation="MHELREAIDRGMLRSRGPLRARLKQLEESARGGMDMSAVLGKLA
                     ADAQRSLEQAEQRRERLPRVTFPEELPISAKRGDIAEAIRSHQVVIVCGETGSGKTTQ
                     LPKICLELWRGVQGMIGHTQPRRIAARSVASRIAAELRTTLGDAVGYQVRFTDKSNSE
                     NFLKVMTDGILLAETQGDRWLAQYDTLIIDEAHERSLNIDFLLGYVKQLLPRRPELKL
                     IITSATIDAERFSKHFDDAPVIEVSGRLYPVDVWYRPFDEDDEDEHEIDLPQAIVDAV
                     DEVTARTSTGDVLVFLPGEREIREAAEALRKHHPQGAEILPLFARLSAQEQERIFAPA
                     GGTRRIVLATNVAETSLTVPGIRFVIDSGLARINRYSYRNKVELLQVEKISQASANQR
                     AGRCGRVMNGICVRLYSEEDFKARAEFGDPEILRSSLASVILRMSALKLGEIEDFPFV
                     DAPASRMIADGYQLLAELGAVDEARKLTPVGSQLAKLPIDPRVGRMLVAAKQEGCLNE
                     ALIIAAALSVQDPRERPMDKQEAVTQAHAEFLDERSDFLSYLEIWKWFEEALKHKKSN
                     RLLVRECHERFLSYLRLREWRELHGQLHALVAEMGWRLNEKAARYEEIHRALLAGLLG
                     NIGFKSEESGIYLGARQIKFVLSPASALKKKGPKWVMAAELTETTRLFARCVAAIEPE
                     WIEKLGQHLAKRSYREPHWSKDSGSVVAYEQVTVYGLVVVPKRRVQYGPVNPKEAWEL
                     FIRGALVAGHYAGNAPFLDHNRRLIEEVQELEHKSRRHDVLVDDEAIFAFYAERIPQG
                     IYSAVDFEKWRREAERDGPKLLFLTRDYLMRHSAEQVTEDLFPDALIVGMQTFDLAYR
                     FEPGHALDGVTMIVPLHLLNQLDERRCEWLVPGLLRDKITYLIKGLPKGMRKHFVPVP
                     QVVTDCMGILEADSGPLTEALSQALFKKIGVEVPVEAWDLADLPPHLQMNFSIVDENG
                     MEIASGRDLPQLRSQLGVKARRTFSAAASAGFERKTLTSWDFDELPEQVEVDRGGRKL
                     VGYPAIADEGRSVALTLLDTEAEAEAATRKGLRRLFQLALPEQMKFLARNLPGFTELQ
                     LRYALLFEREGGKQDKGGRHDKSEVAERLREELITAICDRAFFVEDEPVRNRKAFEVR
                     VAKGKTRLMDVAQEICRIAAEILAEHQALRPKLEQPQHAAWTRVVADIRSQLRELLGP
                     GFLASVPFERLKHYPRYLRAIAVRLDKIASNPERDGNWQQQLARYWQAYQARLAADRS
                     RGLRNPKVEEMRWMLEELRVSLWAQQLKTPYPVSFRRIEKYWSEV"
     gene            6389..8218
                     /locus_tag="VE030_10285"
     CDS             6389..8218
                     /locus_tag="VE030_10285"
                     /inference="COORDINATES: protein motif:HMM:NF024977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZF23837.1"
                     /translation="MPPSKRQPRQTGRTHFAASGRLRIGDDWNAITIIALSQDNPLKA
                     VAEFVENSIDAGARTITITRGRERGEHYLMVADDGGGVPKDADGAPDFRFVATHICDS
                     IKRQLKAGGSKGIQGEFGIGLLSFWTVGEELAMTSSGTDGRVYEMHMRKGDPSYSVTS
                     RNLLVPEAGARLKISPLLPGIRQLSGEKIQWYLASELRERIRQSGARIKVVDRQARKE
                     FNVEPRQFTGELLHHLLAPTPALGEVYLELYLNPADPGNEVGLYRSGTRILRSLTELD
                     AFRKAPWTDGYLQGIIDVPFLNLTPATRTGVIHDAALASFAAAMSPVEEKLAALLDEQ
                     KRAEEERSSRDTLRTIQRAFREAMFALPAEEYDWFDINSRQKGAAADGARRPAADGGE
                     TEGIPVAGENTDTPPQRQFFEYAGPLYSARIAPAACTVAVSASRKLQAFARDASRRSI
                     AGDVDYTWRLLDGAGRLEDASAQYVTYFAPEEPCLVRIGVTARQGNIICDADALITVT
                     DSLLPEARDRLSARHGLPGYTLEHAPGKLWRSRYDAEQNLIVVNSGHRDFIYASRAKA
                     LKLRYLVRLYAKEMVRKNFPGLPADQLLDRLIELSLYTEEHLR"
     gene            complement(8242..>8613)
                     /locus_tag="VE030_10290"
     CDS             complement(8242..>8613)
                     /locus_tag="VE030_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23838.1"
                     /translation="YWNGNKATVTGRPTGNTYQINGQPYSSAEIGSLSGKIDFPSVAP
                     YLGVGLASAPKAGRGMTFSFDLGVLYQREPNVALRVACGTTGRCAQLQSDVAAEQVSL
                     QNDLKDFKFYPVASFGIGYRF"
BASE COUNT         1566 a         2803 c         2625 g         1619 t
ORIGIN      
        1 ccgtcgatga tgcgttcgtc caacctggga ttgcgtatca cctcggcgcg atagaacgac
       61 ggacacagcg tcgccgcgtg cgcgttttcg ccgcacaggc cgcaaccgac gcaaccgtcg
      121 atcaccgtag ccaccggatc gaccctcaac ggatcggggc tgtctttaag tgtcaacgtc
      181 gggcagccag acagccggat gcaggcgtga tcgccggtgc agacatcctc gtcgacgccg
      241 tacttcaccc gcaccacgcg tttttccgcc ttgagcaggc cggcgaccca gggcttgatg
      301 cggcgttgcc gttcgagctg gcattcgccc tcggcgatga tgaccttgag cccgctgtaa
      361 tcggtggtga aggcttcgtc gagcgtcttg cgcatcctct caacgtggta agtgtgcacg
      421 gttcgcatcc atttcacacc gatgccggat agcgtcttct cgatggtgag attgcggtga
      481 agcaggcttt gttgtttatt ttcagccgtg attttcacga cctcgtcggg cgtcgagatc
      541 agttcctgcg taccggtggc cgcggtgtaa ccgttcttga atatcaacag taccgcgtcg
      601 tcgccgttga acaacgccga ttgcacaccg gtgagcagtc cgttgtgcca gaacccgccg
      661 tcgcccatga tcgacagcac cctgcgcttc atcatcggcg acaccccggc gcggctcgcc
      721 aagctcatgc cgtaaccgag gatcgagtgg ccgaaggaga aaggctcgaa ggtggaaaac
      781 gaatggcagc cgatgtcggc ggcaatatgc gtggggccga catcgcgctg cgacagcttg
      841 agcgcggcaa ataccgggcg ctccgggcaa ccgatgcaga attgcggcgg ccgcgccggc
      901 agcggcgccg aaaactgctg caccacttcc tggcggcgct gcgcgttgcc ctgcagccag
      961 cgcttgccgg tcccggcgac accggccggc agatagcgct cggcgaattt ctccagcccg
     1021 ccggccatga cctccacggt atactcgcct gccgccatca gcatgtcctt gccgtggatc
     1081 ggcgtgttga cgtcgagccg gcgcaatgtg gccagaattt cctgttcgat gtattccggc
     1141 tgtccttcct cgacgatcag caccgcacgc ttgccgaggc agaatttcga gatctcctcc
     1201 ggcaccagcg ggaacgtcac gttgagcacg aaaatcggta tttcgcattc gccgaaggca
     1261 tcggccattc caaattgctg cagcgcgcgg accagcgagt tatgcagccc tccctgcaca
     1321 atcagcccga catcgtcgcg cctgccggca aacagctcgt tcaatccgtg ctcggcgatg
     1381 tagcggcgag ccgcgggaat gcgcgcctca cccttgagct tttcgtggcg gaaggtcacc
     1441 ggcggatgcg caagccgggt gtaatcgaac ttcgccggtt cctccatcag atgccgggtc
     1501 gagactgcgg gcgcgacgtt gtccttgcag acgaaactgc cacgcacatg acaggcgcgg
     1561 atgcgcagct ccatcagcgc cggcatgttc gaagcctcgg agagccggaa gccgtcctcg
     1621 accatgcgca ccatgttgga tagttccggg cgcggatcca gcattagcat cgaggatttg
     1681 agcgcgtagg catgggtgcg ctcctggatc acgctggcgc cttcgccgta atcttcaccg
     1741 atcacgatca gcgcgccgcc gatgacgccc ggcgaggcaa gattcgacaa cgcatcggcg
     1801 gcaacgttgg taccgacgat ggatttccag gccaccgcgc cgcgcaacgg atagtgaatc
     1861 gacgcgccga gcatggcagc ggcggaggcc tcgttggaac aggcttcgac gtgtacgccg
     1921 agctcgccca tataatcctc ggcctggatc atgacgtcga tcagatggga gactggcgcg
     1981 ccctgatagc cgccgacgta ggccacaccg gattgcagca aagccttggt gatcgcgaga
     2041 atgccttcgc cgtggaagac ctgtccgttt cccaggcgca gcagctcaat ctcgctcttg
     2101 aaggacttct ccatgacacc ttcccgcccc gtgactcctt gtcctgtccg tgtctggcga
     2161 tgctatcagc cctcgctaga cctcgctcca gtatttctcg atgcgcctga aggaaacagg
     2221 atagggcgtt ttgagctgct gcgcccacag cgacacccgc aattcctcca gcatccagcg
     2281 catttcctcc accttcgggt tgcgtaatcc gcgagagcgg tcggcggcta gccgcgcctg
     2341 ataggcctgc cagtagcgcg cgagttgctg ctgccagttg ccgtcgcgct ccgggttcga
     2401 ggcgatcttg tcgagccgca ccgcgatcgc ccgcaggtag cgcgggtaat gcttgagcct
     2461 ttcgaacggc accgaggcga ggaatccggg tccgagcagc tcgcgcaact ggctgcggat
     2521 gtccgcgact acgcgcgtcc atgccgcgtg ctgcggctgt tcgagctttg gccgcagcgc
     2581 ctggtgttcg gccagaatct cggcggcaat gcggcagatc tcctgcgcca cgtccatcag
     2641 ccgcgtcttg cctttcgcca cccgcacctc gaacgccttg cggttgcgca ccggctcgtc
     2701 ctcgacaaag aaagcgcgat cgcaaatggc ggtgatcagt tcttcgcgca agcgttcggc
     2761 cacttcgctc ttgtcatgcc ggccgccctt gtcctgtttg ccgccttcgc gctcgaacag
     2821 cagcgcataa cgcaactgca attcggtaaa ccccggaagg ttgcgcgcca ggaacttcat
     2881 ctgctcaggc agcgccagct ggaacagccg gcgcaagccc ttgcgggtcg ctgcctcggc
     2941 ctcggcttcg gtatccagaa gcgtcagcgc caccgacctg ccctcgtcgg cgatcgccgg
     3001 ataaccgacc agcttgcgcc caccgcgatc tacctcgacc tgctcgggca actcgtcgaa
     3061 gtcccacgag gtcagcgtct tgcgctcgaa tccggcgctc gccgccgcgc tgaaggtgcg
     3121 ccgcgccttg acgccgagct ggctgcgcag ctgcggcaga tcgcgtccgg atgcgatttc
     3181 catgccgttt tcgtccacga tgctgaaatt catctgcagg tgcggcggca gatcggccag
     3241 atcccacgcc tcgaccggca cttccactcc tattttcttg aacagcgcct gcgacagcgc
     3301 ctcagtgagc ggcccgctgt cggcctcgag aatccccatg caatcggtga cgacctgcgg
     3361 taccggcacg aaatgcttgc gcatgccttt gggcagaccc ttgatcaggt acgtgatctt
     3421 gtcgcgcaac agccccggca ccagccactc gcagcggcgt tcgtcgagct ggttgagcag
     3481 gtgcaacggc acgatcatgg tcacgccgtc gagcgcgtgg ccgggctcga atcggtaggc
     3541 aagatcgaag gtctgcattc ctactatcag cgcatcgggg aaaagatcct cggttacctg
     3601 ctccgcggaa tggcgcatga gatagtcacg ggtcaggaac aacagcttcg ggccgtcgcg
     3661 ttcggcttcc ctgcgccatt tctcgaaatc caccgcactg tagattccct gcggaatgcg
     3721 ttcggcgtag aacgcgaaga tggcttcgtc gtccaccaga acgtcgtggc ggcgcgactt
     3781 gtgttcgagt tcctgcacct cctcgatcaa cctgcggtta tggtccagga acggcgcgtt
     3841 tcctgcatag tggccggcca ccagcgcgcc ccggatgaac agttcccacg cttccttggg
     3901 gttgacaggg ccatactgca cccgccgttt gggcaccacc accaggccgt acaccgtcac
     3961 ctgctcgtag gcgaccacac tgcccgagtc cttcgaccag tgcggctcgc gatagctgcg
     4021 cttggccagg tgctggccca gtttctcgat ccactccggc tcgatcgccg cgacacaacg
     4081 ggcgaacagg cgcgtggtct cggtgagttc cgccgccatc acccatttcg gtcctttctt
     4141 cttcagcgcc gaggccgggc tgagtacgaa cttgatctgg cgcgcgccga ggtagatgcc
     4201 cgattcctcc gacttgaagc cgatgttgcc cagcaagccg gccagcagcg cgcggtggat
     4261 ctcctcgtag cgcgccgcct tctcgttcag ccgccagccc atctccgcca ccagcgcgtg
     4321 caactggccg tgcagctcgc gccattcgcg cagccgcagg tacgacagga aacgctcatg
     4381 gcactcgcgc accagcaggc ggttcgactt cttgtgcttg agcgcttcct cgaaccactt
     4441 ccagatttcc agataggaca gaaaatcgga acgctcgtcg agaaactccg cgtgcgcctg
     4501 cgtcaccgct tcctgcttgt ccatcgggcg ttcgcgcgga tcctgcaccg acagcgccgc
     4561 ggcgatgatc agcgcttcat tcaggcagcc ctcctgtttt gcggcgacca gcatgcgccc
     4621 cacccgcgga tcgatcggca gcttcgccag ctggctgccg accggcgtga gcttgcgagc
     4681 ctcgtccacc gcgccgagct cggccagcaa ttgatagcca tcggcgatca ttctcgaggc
     4741 cggcgcatcg acgaacggaa agtcctcgat ctcgccgagt ttgagcgcgc tcatgcgcag
     4801 gatcaccgag gcaagcgaag agcgcaggat ttcggggtcg ccgaattcgg cgcgggcctt
     4861 gaaatcctcc tcgctgtaga ggcgcacgca gatgccgttc atcacccgcc cgcaacggcc
     4921 cgcgcgctgg ttggccgaag cctgcgagat cttttcgact tgcagcagtt ccaccttgtt
     4981 gcgataactg tagcggttga tgcgggcgag ccccgagtcg atcacgaagc ggatgccggg
     5041 aacagtgagc gaggtctcag ccacgttggt ggcaagcacg atgcggcggg tgccacccgc
     5101 gggcgcgaag atgcgctcct gctcctgtgc ggacagccgc gcgaacagag gcaggatctc
     5161 cgcgccttgc gggtgatgct tgcgcagagc ctcggcggct tcgcggatct cccgctcccc
     5221 cggcaggaac accagcacgt cgccggttga tgtgcgagcc gttacctcgt ccaccgcatc
     5281 gacgatcgcc tgcggaaggt cgatctcgtg ctcgtcctcg tcgtcctcgt caaacgggcg
     5341 gtaccagaca tccaccgggt acagccgtcc tgagacctcg attaccggcg catcgtcgaa
     5401 gtgcttcgaa aacctttcgg cgtctatggt cgcggaagtg atgatcagct tcagctcggg
     5461 ccggcgcggc agcagttgct tgacataccc gagcagaaaa tcaatgttca ggctgcgctc
     5521 atgcgcctcg tcgatgatca gcgtgtcgta ctgggcaagc cagcggtcgc cctgggtttc
     5581 ggcgagcagg atgccgtcgg tcatcacctt caggaagttt tcgctgttgg acttgtcggt
     5641 gaagcgcacc tggtagccga ctgcgtcgcc gagcgtggtc cgcaactcag cggcgatccg
     5701 ggaggccacc gagcgcgcgg cgatccgtcg cggctgggtg tggccgatca taccctgcac
     5761 accgcgccag agttcgaggc agatcttcgg cagttgcgtg gtcttgccgg aaccggtctc
     5821 gccgcaaacg atcaccacct ggtgcgagcg aatcgcctct gcgatgtcgc cgcgcttcgc
     5881 gctgatcggc aattcttcag gaaacgtgac acggggcagc ctctctcgcc gctgttctgc
     5941 ctgttcaagt gagcgctgcg catcggctgc gagcttgccc agcacggcgg acatatccat
     6001 gccgccgcgg gccgactcct cgagctgctt cagacgcgcg cgcagagggc cgcgcgagcg
     6061 cagcatgccg cgatcgatgg cttcgcgcag ctcgtgcaaa tcgtctccag gctgccgctt
     6121 aagatggggc acagggctca tcgggagggt gaaaaactgt gcggcgaaag gcaatacaca
     6181 actagcgatt gtaatcggtt cgcgtcccct gccgccgcga gggatgcagg tccggacact
     6241 ccggacaccg ccgcgtctgg agctatcgcg ggaatatgtg tccactccga tgaacgccag
     6301 agctccggag ttgaaccaga caaagcgcgc catttgcggc tgctttttgt tacatttcca
     6361 ttcttgagca agctgttgga tcaggatctt gccaccttcc aaaagacaac ctcggcagac
     6421 gggccgcact catttcgccg cgagcgggag gctacgcatc ggcgatgact ggaatgccat
     6481 caccattatc gccctgtccc aggacaaccc gctgaaggcc gtcgcggagt tcgtggagaa
     6541 cagcatcgac gcgggcgccc gtaccatcac catcacgcgc ggccgggaac gcggggagca
     6601 ctatctgatg gtcgctgacg atggtggcgg cgtgccgaag gatgctgacg gtgcgccgga
     6661 cttccgcttt gtggcaaccc acatctgtga ttcgatcaag cggcaactga aggctggcgg
     6721 cagcaaaggc attcaaggcg agttcggcat cggattgctg tcgttctgga ccgtgggaga
     6781 ggaactggcg atgacctcgt cgggcaccga cggacgcgtc tacgaaatgc acatgcgcaa
     6841 aggcgacccc agctacagcg taacaagccg caatttgctg gtgcccgaag ccggcgcccg
     6901 cctcaagata tccccacttc tgccagggat ccggcagctt tccggcgaga agattcagtg
     6961 gtatctggct tcggaactgc gcgaacgcat ccgccaatcg ggtgcacgca tcaaagtggt
     7021 ggacaggcag gcgcgcaagg aattcaacgt cgaaccacgg cagttcactg gcgagttgct
     7081 gcatcatctg ctggcaccga cgccagcgtt gggcgaggtc tatctggagc tttatctgaa
     7141 cccggccgat cctggaaacg aagtcggact ctatcgttcc ggcacgcgca ttctgcgttc
     7201 acttaccgaa ctcgacgcgt tccggaaagc gccctggacc gatggttacc tccagggcat
     7261 catcgatgta ccgttcctga atctcactcc cgccacccgt accggcgtga ttcacgacgc
     7321 cgcgcttgcg tccttcgcgg cggcgatgtc accggtggag gaaaagcttg cggctctcct
     7381 cgacgagcag aagcgggccg aagaggaacg tagcagtcgc gatacgcttc gcaccatcca
     7441 gcgcgcattc cgcgaggcta tgttcgcgct gcccgcggag gagtacgact ggttcgatat
     7501 caactcgcgc cagaaaggcg ccgcggccga cggtgcaaga cggcccgcgg cggatggcgg
     7561 agaaaccgaa ggaatcccgg tcgcgggcga gaacacggac accccgccgc aacgccagtt
     7621 cttcgagtat gcagggccgc tctacagcgc tcgcatcgcg ccggcagcat gcactgtcgc
     7681 ggtgtccgca tcacgaaagc tgcaggcgtt cgcgcgcgac gccagccggc gctccatcgc
     7741 aggagacgtg gattacacct ggcgcctgct cgacggggcg ggccggctgg aggatgcaag
     7801 cgcgcagtac gtcacctatt ttgcccccga agaaccatgt ctggtgcgca tcggggtcac
     7861 tgcgcgtcaa ggcaacatca tctgcgatgc agacgcattg atcacggtaa ccgacagcct
     7921 gttaccagaa gccagggatc ggctgtccgc tcgccacggt ctgccggggt acacgctgga
     7981 gcacgcgccg ggcaagctgt ggcgttcccg ctacgacgcc gagcagaacc tgatcgtcgt
     8041 caacagcggg caccgcgact tcatctacgc ttcacgcgcc aaggctctca agctgcgcta
     8101 tctggtgcgt ctttatgcaa aggaaatggt gcgaaagaac tttcccggac ttcccgcgga
     8161 tcagttgctg gatcgattga tagagctatc gctctatacg gaggaacacc tgcgctaggg
     8221 agacgacgcc ggccgccagt ctcaaaagcg gtagccgata ccgaacgacg ccaccggata
     8281 gaacttgaag tccttcaggt cgttctgcag ggatacctgc tccgccgcaa cgtcgctctg
     8341 caactgagcg cagcggccgg tggttccgca ggcaacgcgc agcgcgacat tcggttcgcg
     8401 ttggtagagg actcccaggt cgaaggagaa ggtcatcccc cgccctgcct ttggtgcaga
     8461 cgccaaaccg accccgagat acggtgcaac gctcggaaag tctattttcc cgcttagcga
     8521 accgatctcg gcgctgctat acggttgtcc gttgatctgg taagtgttgc cggtcggtcg
     8581 gccggtcacg gtggccttgt tgccgttcca ata
//
LOCUS       DASQOP010000262         9648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_935045, whole genome shotgun sequence.
ACCESSION   DASQOP010000262 DASQOP010000000
VERSION     DASQOP010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9648
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935045"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="VE030_10295"
     CDS             complement(<1..782)
                     /locus_tag="VE030_10295"
                     /inference="COORDINATES: protein motif:HMM:NF018827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23839.1"
                     /translation="MAEEKPTTPVDTTEFARTLAEIALRSSHLLKEHLAEQSKESGEE
                     PVDELGVGKAFTDLAAKLMTDPFKLAEAGMRMWQDYFMLWQNTMKRAMGDEPVPVVEA
                     PKSDNRFRSELWQNNLVFDYIKQSYLIAAQNIQKTVSEVEGLDAQTARKVKFFTRQYV
                     DALAPTNFVMTNPEVLKTTVETGGKNLLDGLNHLLHDLERGHGKLAISMTDYKAFKMG
                     ENVATTPGKVIYQNELMQLIQYQAATKEVYKTPLLIIPPWIN"
     gene            complement(1000..1665)
                     /locus_tag="VE030_10300"
     CDS             complement(1000..1665)
                     /locus_tag="VE030_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HZF23840.1"
                     /translation="MSELPLRKKIIANALAMNVLGINRGKSGNVSARWKDGFLITPSG
                     LPYEETRPGEIVFVDSKGKPTGKRPPSSEWRFHHDIYRTRKDAQAVVHTHSSFATTLA
                     CLGMGVPAFHYMIAVAGGNSIRCAPYATFGTQELSDHALKALSGRHACLLANHGMIAT
                     GDSLEHALALAVEVEALCEQYWRALQIGKPAILSDAEMERVLEKFRTYGKMQKITAHR
                     RGR"
     gene            complement(1662..2747)
                     /gene="mtnA"
                     /locus_tag="VE030_10305"
     CDS             complement(1662..2747)
                     /gene="mtnA"
                     /locus_tag="VE030_10305"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008542410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HZF23841.1"
                     /translation="MQVDGVAYRSIWRDGDVVRIIDQTLLPHTFRVVKLGSAEEVADA
                     IRNMRVRGAPLIGAAAAFGMAMAMAADASGANLTRAGEQLAKTRPTAINLRWAIGRMA
                     HALRPLAVQDRPEAAWKEAQAIADEDVAMNHAIGQHGVELIRKAAENRSGPVNVLTHC
                     NAGWLATVDMGTALAPIYLAHDQGIPVHVWVDETRPRNQGLLTAWELAHHGVPHTVIA
                     DNAGGHLMQRGQVDISIVGADRVTARGDVCNKIGTYLKALAAKDCGIPFCVAFPGSTI
                     DWDIEDGFVGIPIEERGADELRSVAGRGGDGEPARLWLVPERSPVANPAFDVTPAALV
                     SMLITERGLAPASRAGLSALYPERSQR"
     gene            complement(2747..3808)
                     /locus_tag="VE030_10310"
     CDS             complement(2747..3808)
                     /locus_tag="VE030_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZF23842.1"
                     /translation="MQTPARAARLEAPAPLTFPFPAPPPGGTVHEITSGVFWLRMPLP
                     FALDHINLWLLRDGKGWTAVDCGIAADATRIHWEQIFRDVVGNSGLTRVLVTHFHPDH
                     FGNAGWLTQRFEAPLWMSESEYLNAHAQYQTQAGFGADATARLFAEHGLDPERVAAIK
                     GRGNSYRAIVSEPPTRFLRMMDGDEIDIGGNNWRVIVGWGHAPEHAALYCESLGVLIS
                     GDMLLPKISTNVSVWPTEPTGDPLKLFLRSIDRFLELSADTLVLPSHGLPFRGAHARV
                     QMLHAHHRDRLAEVLESCATPKTARDLLPVLFRRALDTHQTFFAMGESIAHLHYLWYQ
                     GRLTHSQDADGVHRFVRAG"
     gene            3895..4326
                     /locus_tag="VE030_10315"
     CDS             3895..4326
                     /locus_tag="VE030_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZF23843.1"
                     /translation="MADSKFTITELAREFGLTTRAIRFYEDQGLLAPGRAGRNRVYGH
                     RDKVRLKLTLRGKRLGLSLSEIRELIDLYDAAKDEQVQVERFLEVLERRRAILEQQRE
                     DIEAVLGEIAAFEKQCRDFLAGKGKKPERGAAGKPGIKAKS"
     gene            4525..4974
                     /locus_tag="VE030_10320"
     CDS             4525..4974
                     /locus_tag="VE030_10320"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZF23844.1"
                     /translation="MSGFEPGDPVYADRVRASFDRQKIMALIGAQLVRLAPGECEIHL
                     PFKPELSQQRGYYHGGIIGTIADSAGGYAAFTLMPRDASVLTVEYKMNLLAPGDGELL
                     IARGRVLKAGRTLVVARVDAGVVKNGRETLCATLLQTLMTMHGRPDQ"
     gene            4971..6155
                     /locus_tag="VE030_10325"
     CDS             4971..6155
                     /locus_tag="VE030_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HZF23845.1"
                     /translation="MKPETFFKSYPTLNFGLGEDIDMLRDTVQNFASKEIAPRAAEID
                     HNNEFPMDLWRKLGGLGLLGVTVEEEYGGTNMGYLAHVVAMEEISRASGSVGLSYGAH
                     SNLAVNQIRRNGNEAQKRKYLPKLISGEHVGALAMSEPNSGSDVVSMKLRAEKKGDRY
                     ILNGNKMWITNGPDANVLVVYAKTDVNAGPKGMTAFIVEKGCKGFSTAQKLDKLGMRG
                     SNTCELVFRDCEVPTENVLGEVNRGVHVLMSGLDYERAVLAGGPLGIMQACMDVVMPY
                     VHERQQFGQPIGEFQLMQGKIADIYTTMNACKAYVYAVGRACDRGETMRKDAAGAILY
                     AAEKATWMAGEAIQALGGNGYVNEYATGRLWRDAKLYEIGAGTSEIRRMLIGRELFKE
                     TS"
     gene            6343..8115
                     /gene="aceK"
                     /locus_tag="VE030_10330"
     CDS             6343..8115
                     /gene="aceK"
                     /locus_tag="VE030_10330"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HZF23846.1"
                     /translation="MTTVTAQTQQDLAFLIAQGMLDGFNRHYRLFRETSRFAKSLFEA
                     GQWAEIQRISRERIQFYGRRVTETVEYLKRRFNTDHLPDTILQQVKLHYISLLANHKQ
                     PELAETFFNSVSCRILQRSYYHNDFLFVRPAISTEHMDSDPPTYRSYYPAKSGLRATI
                     AQVLRDFGLRNPFRDLHRDTRWLLRAWRAHLPRPFLPDANHQIQVLTSLFFRNKGAYA
                     IGKIINGNHEYPFAIAILRNDDGSIYLDTVLLDTEQLSILFGFSRAYFMVDMEVPSGY
                     VEFLKSLLPNKPAAEIYTTLGLQKHGKTLFYRDFLHHLKHSSDDFVIAPGIRGLVMLV
                     FTLPSYPYVFKVIKDVISPPKDTDRRQVMEKYLLVKYHDRVGRMADTLEYKDVAFPKD
                     RFAPGLLEELRALAPTVIEEEGDTLVVKHVYIERRMVPLNMHLDGANDDQVAHAIRDY
                     GNAIKQMAAANIFPGDLLFKNFGVTRFGRVVFYDYDEIAYMTDCTFRRMPAPRTPEDE
                     MSAEPWYPVGAHDVFPEEFAAFLLGDARVRKVFLEHHADLLDADHWNVIKQHIAAGHI
                     EDVFPYPEALRFRNRFARRTTHTT"
     gene            8301..9488
                     /locus_tag="VE030_10335"
     CDS             8301..9488
                     /locus_tag="VE030_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HZF23847.1"
                     /translation="MANDPIVIVSAARTPMGALQGELKDFAAPQLGAAAIRAAIERAG
                     VKPDQVQEVIMGCVLPAGQGQAPARQASLGAGLPLSAGCTTVNKMCGSGMKAAMLAHD
                     LLVVGTNDVMVAGGMESMSNAPYLLPKARSGYRMGHQQVLDHMFLDGLEDAYEKGRLM
                     GSFAEDCAAKYQFSREDQDKFAVASLERAQKANRDGSFAWETVPIAIKAGKDERFVEH
                     DESPFKANFDKIPLLKPAFRKDGTVTAANSSSISDGGAALVMTRRSTADRLGLEPLAT
                     IAGHSTHAQEPGWFTTAPVGAIRKLFDKTGWGADQVDLYEINEAFAVVTMAAMKEHKL
                     PHEKVNVHGGACALGHPIGASGARILVTLIGALRKRGGKRGVASLCIGGGEATAMAIE
                     LVA"
     gene            9544..>9648
                     /locus_tag="VE030_10340"
     CDS             9544..>9648
                     /locus_tag="VE030_10340"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HZF23848.1"
                     /translation="MANKKIGFIGVGMMGHGMAKNLVEKGFPTTILGHK"
BASE COUNT         1879 a         2894 c         3032 g         1843 t
ORIGIN      
        1 ttgttgatcc acggcggaat gatcagcagc ggcgtcttat agacctcctt cgtggccgcc
       61 tgatactgga tcaactgcat cagttcgttc tggtaaatga cttttcccgg cgtggtggcg
      121 acgttctcgc ccatcttgaa cgccttgtag tcggtcatgc tgatcgccag cttgccgtgg
      181 ccgcgttcga ggtcgtggag cagatggttc aggccgtcga gcaggttctt ccccccggtc
      241 tccacggtgg tcttcagcac ctcgggattg gtcatcacga aattggtcgg ggccagcgcg
      301 tccacgtact ggcgggtgaa gaacttcacc ttgcgcgcgg tctgcgcgtc gaggccttcg
      361 acttcggaga cggtcttctg gatattctgc gcggcgatca ggtaggactg cttgatgtaa
      421 tcgaacacca ggttgttctg ccaaagctcg ctgcggaagc ggttgtcgga tttgggagct
      481 tccacgaccg gcaccggttc atcgcccatg gccctcttca tggtgttctg ccacagcatg
      541 aagtagtcct gccacatgcg catcccggcc tcggcaagct tgaacggatc ggtcatcagc
      601 ttcgccgcca gatctgtaaa ggccttgccc acgccgagtt cgtccaccgg ttcctcgccg
      661 gactccttcg actgttccgc gagatgttcc ttgaggagat gactgctgcg cagcgcaatc
      721 tcagcgagcg tgcgcgcgaa ttccgtcgta tcgacggggg tggttggttt ttcttcggcc
      781 atagcggcta agctaggcgc aactggatga cagcgcaagc gggcgtttta aaaaaaccgg
      841 cgctcaccac agaggacgcc gaggacgcgg aggaagggca agagcaaaaa aacatccgag
      901 agtaaaaccg ggtatctgtg gttacttcgg cgccaggctc gcttttgtct ccgacatggc
      961 tttcctcgta tttgcttttc ctctgcgtcc tcagcgtcct cagcgtcctc tgcggtgagc
     1021 ggtgatcttt tgcatctttc cgtaggtcct gaatttctcc agcacacgtt ccatctcggc
     1081 gtcggaaaga atggcgggct ttccgatctg aagcgcgcgc cagtactgct cgcataatgc
     1141 ttcgacttct acagccaggg ccagcgcgtg ctcaagactg tccccggtcg cgatcattcc
     1201 gtggttggcc aggaggcagg catggcggcc tgacagcgcc ttcaacgcgt gatccgacaa
     1261 ttcctgggtg ccgaaggtgg cgtatggcgc gcaacggatg ctgttgccgc cggcaactgc
     1321 gatcatgtag tgaaacgcgg gcacgcccat cccgagacag gcgagcgtgg tggcgaaact
     1381 cgaatgggta tgaactacgg cttgcgcatc cttgcgcgtg cggtagatgt cgtgatggaa
     1441 gcgccactcc gaagacgggg gccgcttgcc cgttggtttg cccttactgt cgacgaagac
     1501 gatctcgcca ggcctggttt cctcgtacgg caggccggat ggtgtgatca ggaaaccgtc
     1561 cttccagcgc gcgctgacgt tgccggactt gccgcggttg atgccgagca cattcatcgc
     1621 cagcgcgttg gcgatgatct tcttcctcaa aggcagttcg ctcatcgctg cgagcgttcc
     1681 ggatagagcg ccgacaaacc cgcgcgcgag gccggcgcga gcccgcgctc ggtgatcagc
     1741 atggacacca gcgcggccgg cgtaacgtcg aaggccggat tggcgaccgg gcttctctca
     1801 ggaaccagcc acaagcgcgc tggttcaccg tcgccgccac ggcccgcgac cgatcgcagt
     1861 tcatcggctc cgcgctcttc tatcgggata cccacgaatc cgtcctcgat gtcccagtct
     1921 atagtcgagc ccggaaacgc cacgcaaaac gggatgccgc aatccttcgc tgccagcgcc
     1981 ttcagataag tgccgatctt gttgcagacg tcgccgcgcg cggtgacgcg gtccgccccg
     2041 acgatcgaga tatccacctg accccgctgc atcaggtgcc cgccggcgtt gtccgcaatc
     2101 accgtatgcg gcacgccgtg atgcgcgagt tcccaggcgg tgagcaatcc ctgattgcgc
     2161 gggcgggtct cgtccaccca cacgtgcacg gggatgccct ggtcgtgcgc aaggtagatg
     2221 ggcgcgagcg cggtgcccat gtccacggtg gccagccagc cggcgttgca gtgggtgagc
     2281 acgttgacgg gtccggaacg attctccgca gccttgcgaa tcaactcgac gccgtgctgt
     2341 ccgatcgcgt gattcatcgc cacgtcttcg tcggcgatcg cctgcgcttc cttccatgct
     2401 gcttcggggc gatcctggac cgcgagcggc cgcagcgcat gggccatgcg cccgattgcc
     2461 cagcgcaggt ttattgcggt cggacgggtt ttggcgagct gttcacctgc gcgggtcaaa
     2521 ttggcgccgg aggcatcggc cgccatcgcc atggccatgc cgaatgctgc ggcagcgccg
     2581 atcagcggcg cgccacgtac ccgcatgttg cggatcgcgt cggccacttc ttccgcgctg
     2641 ccgagtttga cgaccctgaa ggtatgcggc agcaaggtct gatcaatgat gcgcacgacg
     2701 tcgccatcgc gccagatgct tcggtacgcg acgccatcga cctgcatcaa ccggccctga
     2761 cgaaccggtg aacgccgtcc gcgtcctgcg agtgcgtcaa ccggccctga taccagagat
     2821 agtgcagatg cgctatggat tcccccatcg cgaagaacgt ctggtgcgtg tcgagtgcgc
     2881 ggcggaacaa tactggtagc agatccctgg cggtcttcgg cgtggcgcag gattcgagca
     2941 cttccgcgag gcggtcgcga tgatgcgcgt gcagcatctg cacccgcgca tgcgcgccgc
     3001 ggaacggcag gccgtgggaa ggcagcacca gtgtatcggc agacagttcg agaaagcggt
     3061 cgatcgagcg caggaaaagc ttcaatggat cgcccgtggg ctcggttggc cagacgctga
     3121 cgttggtgct gattttcggc aacagcatgt cgccggagat caatacgccc agcgactcgc
     3181 agtacagcgc cgcgtgttcg ggggcgtgtc cccagccgac gatgacgcgc cagttgttgc
     3241 cgccgatgtc gatctcgtcg ccatccatca tgcgcaagaa ccgggtgggt ggttcggaga
     3301 cgatcgcacg gtaggaattg ccccggccct tgatcgctgc gacgcgttcg gggtcgagcc
     3361 cgtgttcggc gaacaatcgc gcggtggcat ccgctccaaa gcccgcctgg gtttggtact
     3421 gggcatgggc attcaggtac tcggactcgc tcatccacag cggcgcttcg aatcgctgag
     3481 tcaaccaacc ggcgttgcca aaatggtccg gatgaaagtg cgtaaccagc acccgcgtga
     3541 gacctgaatt gccgactacg tcgcggaaga tctgctccca gtgaatgcgg gttgcgtcgg
     3601 cagcgatccc gcagtccacc gctgtccaac ccttgccgtc gcgcagcagc cagaggttga
     3661 tgtggtcgag cgcgaacggc agcggcatgc gcagccagaa cacgccggac gtgatctcgt
     3721 gcacggttcc tcccggaggc ggtgccggga acgggaaggt aagcggtgcc ggcgcttcga
     3781 gtcgcgccgc gcgagcgggt gtctgcatgc gtggattcag gggaagtatt gggcgcggag
     3841 gtgtgccgcg aactagttgg attctacttt acgtttacgt aaactgcgtc gcgcatggcg
     3901 gactccaaat tcacaattac cgaacttgcc cgggaattcg gcctgacgac gcgggcgatc
     3961 cgcttctacg aagatcaggg cctgctcgcg cccgggcgcg ccggacgcaa ccgggtttac
     4021 ggccaccgcg acaaggtccg cctcaagctt accctgcgcg gcaagcgcct cgggctctcg
     4081 ctgtccgaaa tccgtgagct gatcgatctg tacgacgccg ccaaggacga gcaggtccag
     4141 gtcgaacgtt tcctcgaagt gctggaacgg cggcgcgcta tcctcgagca gcagcgggag
     4201 gacatcgagg cggtattggg cgaaattgct gcgttcgaga aacagtgccg ggattttctg
     4261 gccgggaaag gcaaaaagcc ggaacggggt gccgctggca aaccggggat caaagcaaaa
     4321 tcctgattgc atggaattgt tgcgcacaaa atcgctacgg gcttgctgcg aggcgactgt
     4381 tcggggccgg acatctccag atgggccgac atgccgactt ttagagttaa tgacctgatc
     4441 tgaaatgatt tttaagtaat accgcgacta cttattgacg ttaacgtaaa cgtcaattac
     4501 agtctctcgg aatggagagc acgtttgagc gggtttgagc cgggggatcc cgtctacgcc
     4561 gatcgggtgc gggcgagttt cgatcgccag aaaatcatgg cgctgatcgg cgcgcagtta
     4621 gttcggctgg cgcccggcga gtgcgaaatc catttgccct tcaagcccga actatcgcag
     4681 cagcgcggct actaccatgg cggcatcatc ggtaccatcg cagattcggc gggcggctac
     4741 gcggcgttta ccctgatgcc gcgggacgcg agtgtcctga cggtggagta caagatgaac
     4801 ctgctggcgc cgggcgatgg cgaactgctg atcgcccgcg gcagggtgct caaggccggc
     4861 cgcacgctgg tggtggcgcg ggtggatgcc ggcgtggtca agaacggccg cgaaactctg
     4921 tgcgccacat tgctccagac cctgatgacg atgcacggga ggcccgatca atgaagcccg
     4981 aaaccttttt caagagctat ccaacgctca atttcggcct cggcgaggac attgacatgc
     5041 tgcgcgacac cgtgcagaac ttcgcttcca aggagatcgc cccgcgcgcg gccgagatcg
     5101 accacaacaa cgaattcccg atggacctgt ggcgcaagct tggcggccta ggactgctcg
     5161 gcgtcaccgt cgaggaggaa tacggtggca ccaacatggg ctatctcgcc cacgtggtgg
     5221 cgatggaaga gatttcccgc gcctcgggct cggtcgggct gtcctatggc gcgcactcga
     5281 acctggcggt gaaccagatc cgccgcaacg gcaatgaggc tcagaagcgg aagtacctgc
     5341 cgaagctgat ttccggcgag cacgtcggtg cgctggcgat gagcgagccg aattccggct
     5401 ccgacgtggt gagcatgaag ctgcgtgccg agaagaaggg cgaccgctac atcctcaacg
     5461 gcaacaagat gtggatcacc aacgggccgg acgcgaacgt actggtggtg tacgccaaga
     5521 ccgacgtaaa tgccggaccg aagggaatga ccgccttcat cgtcgaaaaa ggctgcaagg
     5581 gcttctccac cgcgcagaag ctcgacaagc ttggaatgcg cggctccaac acctgcgaac
     5641 tggtgttccg ggactgcgaa gtgccgacgg agaacgtgct gggtgaagta aaccgtggcg
     5701 ttcacgtgct gatgagcgga ctcgactacg agcgcgccgt gctcgccggc ggaccgctcg
     5761 gcatcatgca agcctgcatg gatgtggtaa tgccgtatgt gcacgagcgc cagcagttcg
     5821 gccagccgat cggtgagttc cagctgatgc agggaaaaat cgccgacata tacaccacga
     5881 tgaacgcctg caaggcctac gtgtacgcgg tgggcagggc ctgcgatcgc ggcgagacca
     5941 tgcgcaagga cgccgccggc gccattctct acgctgcgga aaaggcgacc tggatggcgg
     6001 gtgaagcgat ccaggcgctg ggcggcaacg gctatgtcaa cgagtacgca accggcaggc
     6061 tgtggcgcga tgcgaagctg tacgagatcg gcgccggtac cagcgaaatc cgccgcatgc
     6121 tcatcggccg cgagcttttt aaagaaactt cttgaagcgc tcaacgcaga ggacgcggag
     6181 gacgcagagg aaggcctgaa cagaaatgaa tccaattgat cggtgcggga accggacaac
     6241 ggggtacgtc agtttctttg gtgttcacct gcttgttttc ttttacctct gcttttcttc
     6301 cgcgtcctcc gcgtcctccg cgatgagagc ttttggatag cgatgacgac cgtcacggca
     6361 cagacccagc aggaccttgc attcctgatc gcacagggaa tgctggacgg cttcaaccgt
     6421 cactaccggt tattccgcga aaccagccgc tttgcgaagt cactattcga ggcggggcag
     6481 tgggcggaga tccagcgtat ctcgcgcgag cgcattcagt tctacggccg ccgcgtgacc
     6541 gagacggtcg aatacctgaa acgccggttc aacaccgatc accttcccga cacgatcttg
     6601 cagcaggtca agcttcatta catctcgctg ctcgccaatc acaaacagcc ggagcttgcc
     6661 gaaacgtttt tcaactccgt gagctgtcgc atcctgcaac gcagctatta ccacaacgat
     6721 ttcctgttcg tgcgcccggc catctctacc gagcacatgg attccgaccc gccgacctac
     6781 cgcagttatt acccggcaaa atccggactg cgggctacca tcgcacaggt cctgcgggac
     6841 ttcgggttgc gtaatccgtt ccgggacctg catcgcgaca cgcgctggct gctgcgtgca
     6901 tggcgcgcgc acctgccgcg gccgttcctg ccggacgcca accaccagat ccaggtgctg
     6961 acgtccctgt tcttccgtaa caagggcgcg tacgcgatcg gcaagatcat caacggtaac
     7021 cacgagtacc cgtttgcgat cgcgatcctg cgcaacgacg acggcagcat ttacctcgat
     7081 accgttctgc tcgataccga gcagctttcc atcctgttcg gcttcagccg tgcgtacttc
     7141 atggtggaca tggaggtgcc gtcggggtac gtggagttcc taaagagcct gttgcccaac
     7201 aagcccgcgg ccgagatcta caccacgctc ggcctgcaaa aacacggcaa gacgcttttc
     7261 tatcgcgact tcctgcatca cctgaaacac tcgagcgacg acttcgtgat cgcgcccggc
     7321 atccgcggtc tggtgatgct ggtgttcacg ctgccttcgt acccgtacgt attcaaggtg
     7381 atcaaggacg tcatctcccc gcccaaggac accgaccgca ggcaggtgat ggaaaagtac
     7441 ctgctggtga agtaccacga ccgcgtgggg cgcatggcgg atacgctcga gtacaaggac
     7501 gtggcgtttc caaaagatcg atttgcaccc gggttgctgg aggagctacg agcgctcgcg
     7561 ccgaccgtga tcgaagagga aggcgacacg ctggtcgtca agcacgtgta cattgaacgg
     7621 cggatggtgc cgctcaacat gcatctggac ggtgccaacg acgatcaggt ggcgcacgcc
     7681 atccgcgatt acggcaacgc catcaagcag atggcggcgg caaacatttt cccgggcgac
     7741 ctgctgttca agaacttcgg cgtgacccgt ttcgggcggg tggtgttcta cgactacgac
     7801 gagatcgctt atatgacgga ttgcaccttc cgccgcatgc cggcgccgcg taccccggaa
     7861 gacgagatgt ccgccgagcc ctggtacccg gtcggtgccc acgacgtgtt ccccgaggag
     7921 ttcgctgcat tcctgctggg cgacgcgcgg gtgcgcaagg tgttcctcga gcatcacgcc
     7981 gacctgctcg acgccgatca ctggaacgtg atcaagcagc atatcgccgc cggacatatc
     8041 gaagacgtgt tcccgtaccc ggaggccttg cgcttccgta accgcttcgc tcgccgtacg
     8101 acgcatacga cttgaaaagc aaaggacgca ggtatgccaa ggagacccaa acgctgatca
     8161 agtgaagaat gaagacatga cgtgacagtg gtggggcttt ttctccggcg atcttgtagg
     8221 gtactttgtc gagaattgat tttccttttg cgtacctttg cgtcctttgc gttgatagct
     8281 ttgttgctct aggaggagtt atggctaacg atccgatcgt cattgtttcc gcggcgcgca
     8341 cgccgatggg ggcgctgcag ggtgaattga aagacttcgc tgcgccgcaa ctcggtgcgg
     8401 cggcgatccg tgctgcgatc gaacgcgccg gcgtcaagcc cgaccaggtg caggaagtga
     8461 tcatgggctg cgtgctgccg gccggacagg ggcaggcacc tgctcgccag gcatcgcttg
     8521 gtgcgggcct gccgctgtcg gcaggttgca ccaccgtgaa caagatgtgt ggctcgggga
     8581 tgaaagcggc tatgctcgcg cacgatctgc tcgttgtcgg caccaacgat gtcatggtcg
     8641 ccggcgggat ggagagcatg agcaacgcgc cctatctgtt gcccaaggca cgctcgggct
     8701 accgcatggg gcaccagcag gtgctggacc acatgttcct cgacgggctg gaggatgcct
     8761 acgaaaaagg caggctgatg ggcagcttcg ccgaggactg tgcggcgaag taccagttca
     8821 gccgggaaga ccaggacaaa tttgccgttg cgtcgctcga gcgcgcgcag aaggccaaca
     8881 gggacggttc gttcgcatgg gaaaccgtgc cgatcgcaat caaggccggc aaggatgaac
     8941 gcttcgtcga gcatgacgaa tcgccgttca aagccaactt cgacaagatt ccgctgctca
     9001 aacccgcgtt caggaaggac ggcacggtga ctgcggcgaa ttcgagttcc atttcggacg
     9061 gtggcgcggc cctggtgatg acgcgccgtt cgaccgccga caggctcggc ctcgagccac
     9121 tcgcaaccat tgccggtcat tcgacccacg cccaggaacc gggatggttt accacggcgc
     9181 cggtcggcgc gatcagaaaa ctgttcgaca agaccggctg gggcgcggac caagtcgatc
     9241 tgtacgagat caacgaagcg ttcgccgtgg tgaccatggc ggcaatgaaa gagcacaaac
     9301 tgccgcacga aaaagtgaac gtccacggtg gcgcctgcgc gctcggacat ccgatcggcg
     9361 cctccggcgc gcggattctc gtaacgctga tcggcgcact gcgcaagcgt ggcggcaaac
     9421 gcggcgtcgc gtcgctctgc atcggcggcg gcgaagccac cgccatggcc atcgaattgg
     9481 tcgcatagcg accaaggaaa atgcattcaa cgagcgtggt ttgttctttc aggagagagt
     9541 gtgatggcga acaagaaaat agggttcatc ggcgtgggca tgatgggcca cggcatggcg
     9601 aagaacctgg tcgagaaggg tttcccgacc actatcctcg gccacaag
//
LOCUS       DASQOP010000263         5155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_128265, whole genome shotgun sequence.
ACCESSION   DASQOP010000263 DASQOP010000000
VERSION     DASQOP010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128265"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="VE030_10345"
     CDS             complement(<1..1097)
                     /locus_tag="VE030_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZF23849.1"
                     /translation="MNDQLDSGAQVDARAAALESRVIAWRRDLHQHPELSNREVRTAG
                     VVAGHLQALGLRVQTGVAHTGVVGLLDSGRPGPVIALRADMDALPVTEQVDLPFASRT
                     RANFNGQEVGVMHACGHDCHVAILMGVADLLAGMKQQLSGSIKFIFQPAEEGPPPGEE
                     GGAALMIRQGVLENPKPEVIFGLHVFAGVEAGTIAYRPGPAMASSDRIRVTVTGRQTH
                     GALPWRGVDPIVISSQIVLGLQTIVSRQVDVSLEPAVVSIGAIKGGVRDNIIPDTVEM
                     LGTVRTFNEDMRVDIHARICNTVEMIARSAGAAAQVHFDNAYPVTVNDVPLTERMVPT
                     LQRVAGADKVFVGQKITGYEDFSFYQQKIPG"
     gene            complement(1100..1930)
                     /locus_tag="VE030_10350"
     CDS             complement(1100..1930)
                     /locus_tag="VE030_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HZF23850.1"
                     /translation="MKQALQSVFRVLLLPLIATVAMAAPAQAQDAEAVIDKAMTGSHR
                     PEANKARDKYRHPKETLLFFGLKPNMTVVEITPGAGWYTEILAPVMRGGIYYAALSRV
                     TEQSSDAQRANDKTFRAKLSGDADLYGKVQLSVFEPNAIQVAPAGSADMVLTFRNVHN
                     WAKAGTADVMFKAFYDALKSGGVLGVVEHRAKPGTTFEHQIDSGYMTEVYVTETAQKA
                     GFKLDNKSEINANPRDTADYPGGVWTLPPTLRNVADGQKPKYLAIGESDRMTLKFVKP
                     "
     gene            complement(1972..2376)
                     /locus_tag="VE030_10355"
     CDS             complement(1972..2376)
                     /locus_tag="VE030_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23851.1"
                     /translation="MRLSPIPLLLLAIVAGPVPADEAGDRIERANLLRKLLEDGPPVH
                     AGESDSRAELPAARLEVDQQTAAERLQVELFQDRQWRQLLESQRANANRPDPLPQLPG
                     TAGLAFEREQRARDLAIQIQRQDLEFRHNSRR"
     gene            2697..2855
                     /locus_tag="VE030_10360"
     CDS             2697..2855
                     /locus_tag="VE030_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23852.1"
                     /translation="MKNLLNSLFKWFNADENRELEAYLSQAANPADVDRLLREWEKRK
                     DKSLFYLP"
     gene            2904..3917
                     /locus_tag="VE030_10365"
     CDS             2904..3917
                     /locus_tag="VE030_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZF23853.1"
                     /translation="MKFGKRKVMTLALQGGGALGALTWGVLDRLLEEPKLKIEAISGT
                     SAGAINAATLASGYARGGREGARKSLDDFWTSVGTASYAIGSSTGVHRALLPNPQKLL
                     LGLTRYFSPYQLNPFDINPLRAIVAKVVDFDAVQSRRAIRLFVGATRVRTGTLRLFEN
                     RELTSDALLASACLPTLHHAIEIDGEPYWDGGYSGNPAVYPLIYRCNTPNILVVMLQP
                     LTRQDPPTSAQAIRSRLTEFQFNSSFLREMRSIALAKQAIDAQWLPVGRLERRFRKLN
                     FHLIETDDLLARMNVDKALNADLGFLLKLKEEGRTRAGTWLGKHFGQVGGESSIDLVK
                     LFG"
     gene            complement(3959..4657)
                     /gene="urtE"
                     /locus_tag="VE030_10370"
     CDS             complement(3959..4657)
                     /gene="urtE"
                     /locus_tag="VE030_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020832263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HZF23854.1"
                     /translation="MLKVEKLNQFYGSSQTLRNVSFEVPTGAVTALLGRNGVGKTTLL
                     KCLMGALPVASGSIALDGQEVSTLPPYARVRLGVGYVPQGREIFPRLTVEENLMMGLA
                     SRKNGTAKDIPGEIYEMFPVLKTMRNRRGGDLSGGQQQQLAIGRALAMKPKVLILDEP
                     TEGIQPSIIKDIERVIQALSDRGNMGILLVEQYYDFARELADNYVVLRRGEVLMSGAG
                     ADMETDNVRSFLSV"
     gene            complement(4826..>5155)
                     /locus_tag="VE030_10375"
     CDS             complement(4826..>5155)
                     /locus_tag="VE030_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZF23855.1"
                     /translation="QGEAGRLAGLLSHGQKQWLEIGMLLMQEPRLLLVDEPVAGMTDE
                     ETERTAELFKSLAGERSVVVVEHDMEFIGSIASVVTVLHEGAVLAEGTMDAVKNDERV
                     IEVYLGR"
BASE COUNT          958 a         1674 c         1483 g         1040 t
ORIGIN      
        1 aatcccggaa tcttctgctg atagaaggag aagtcctcgt aaccggtaat tttctggccg
       61 acgaagactt tgtctgcccc cgccacccgt tgcagggtcg gcaccatgcg ctcggtcagc
      121 ggtacgtcgt tcaccgtgac cggataggcg ttatcgaaat gcacctgcgc ggctgccccc
      181 gcactcctgg cgatcatctc caccgtattg cagatgcggg cgtgaatgtc cacgcgcatg
      241 tcctcgttga acgtgcgcac ggtacccagc atctccaccg tgtccggaat gatattgtcc
      301 cgcaccccgc ccttgatcgc gccgatgctg accaccgccg gctccaggct cacgtccacc
      361 tggcggctga cgatagtctg aagcccaagc acgatctgcg atgaaatcac gatcgggtcc
      421 actccgcgcc acggtagcgc gccgtgcgtc tggcgcccgg tcaccgttac ccggatacga
      481 tcggaactgg ccatcgccgg gccgggccga taggcaatcg tgccggcttc gacccctgcg
      541 aacacgtgca ggccgaaaat cacctcgggt ttcggatttt ccagcacacc ctgcctgatc
      601 atcagcgccg cgccgccttc ctcgcccggc ggcgggcctt cctcggcagg ctggaagatg
      661 aacttgatgc tgccggaaag ctgctgcttc atgcccgcca gcaaatcggc gacacccatc
      721 aggattgcga catggcaatc gtggccgcaa gcgtgcatga cgccgacttc ctggccattg
      781 aaatttgcgc gggtcctgga ggcaaacggc agatccactt gctccgttac cggcagcgcg
      841 tccatgtccg ctcgcaatgc gatgaccggc cccggacggc cgctatccag caacccgacc
      901 acgccggtgt gcgcgacccc ggtctgtacc ctcagtccaa gcgcttgcag atgcccggca
      961 acaacgccgg cggtgcggac ctcgcggttg gacagctccg ggtgctggtg caaatcccgg
     1021 cgccaggcga tgaccctgga ttcgagcgcc gctgcgcgcg cgtcgacctg tgcgccggaa
     1081 tcgagttgat cgttcatcgt cagggtttca cgaatttgag ggtcatgcgg tcggattcgc
     1141 cgatggcgag atacttcggc ttctgcccgt cggcgacatt gcgcaaagtc ggcggaagcg
     1201 tccagacccc gccgggatag tccgccgtgt ccctcggatt ggcgttgatt tcggacttgt
     1261 tgtcgagttt gaacccggcc ttctgtgcgg tctcggtgac gtagacctcg gtcatgtagc
     1321 cagagtcgat ctgatgctcg aaagtagtgc cgggcttagc ccggtgctcg acgacaccca
     1381 gcaccccgcc ggatttgagg gcgtcataaa aggccttgaa catcacatcc gcggtgccgg
     1441 ccttggccca gttatggacg ttgcggaagg tcagcaccat gtccgcgctg cccgccggcg
     1501 caacctggat cgcgttcggc tcgaacaccg agagttgcac cttgccatag agatcggcgt
     1561 cacccgagag tttggcgcgg aaggtcttat cgttggcacg ctgggcgtcc gaggattgct
     1621 cggttacccg ggatagggcc gcatagtaga tccctccacg catgaccggc gccaggatct
     1681 cggtgtacca gcccgcgccc ggggtgattt cgaccaccgt catattcggc ttcaagccga
     1741 aaaacagcag cgtttccttg ggatggcggt acttgtcgcg cgccttgttg gcttccggcc
     1801 gatggcttcc ggtcatcgcc ttatcgatca ccgcttccgc gtcctgtgcc tgcgctggcg
     1861 ccgccatcgc aacggtggcg atcagcggca ataacagcac gcgaaaaacg gattgcaacg
     1921 cctgcttcat gaatctcccc ttcgttcctg gttcatgtgg ttcctgcgcg tctagcggcg
     1981 actgttgtga cgaaattcga gatcctgccg ctggatctga atcgccaggt cgcgtgcccg
     2041 ctgctcgcgt tcgaatgcca gccctgctgt ccccggcaac tgaggcagcg gatccggcct
     2101 gtttgcattc gcccgctggg attccaacag ctgccgccac tggcgatcct gaaagagttc
     2161 gacctgcagc ctctcggcgg cggtttgctg atccacctcc agcctcgcgg cgggcaattc
     2221 ggcgcgcgaa tcggactctc cggcatgtac aggcgggccg tcttcgagca actttcgcaa
     2281 aaggttggcg cgctctatcc ggtcaccggc ttcgtccgcc ggaaccggtc cggcaacgat
     2341 ggccagaagc agcaatggaa tcggggagag tcgcactttt gtgtgaccgg cgaaggcgcg
     2401 ccgagtttac aagcaggccc gggaaccgtc cattttgtgc taccggccac gaccacagac
     2461 tgccaatcct gcggaccgtg tctcgattaa agagacgcag caataatcct cgataaatac
     2521 tatatgtttt atacagatat ataactcttc agatacttta gattgcgact tcttgcattg
     2581 cgacatcaat acaccacgct gtgaaactat ctgataaaat ccattgtcga aataacgctg
     2641 tatgaaacta attaaccgat cttgcactgc cgcaacatag cgaaaggacc aacaccatga
     2701 aaaacctgct caattccctg ttcaaatggt tcaacgccga tgaaaatcgg gagctcgagg
     2761 cttacctgtc gcaggccgcc aatcctgccg atgtggaccg cctgttgcgc gaatgggaga
     2821 aaaggaagga caagtccctc ttctatctgc cgtaagccag ccttgatttt ccgccacgcc
     2881 cggccgtcgc cgccccttcc cgagtgaagt tcggaaagcg caaggtcatg acgcttgccc
     2941 tccagggcgg cggggcactc ggcgcgctga cctggggagt gctcgatcgc ttgctcgagg
     3001 aacctaagct caagatcgaa gcgatcagcg ggactagtgc cggcgcgatc aacgccgcta
     3061 cgctggcgtc cggctacgca aggggtggcc gcgaaggcgc tcgcaaatcg ctggacgact
     3121 tctggacttc ggtgggcacg gcgtcctacg cgatcggctc gtccaccggc gtgcaccggg
     3181 cgctgttgcc aaacccgcag aaactcctgc tcggcctcac ccgttatttc tccccctatc
     3241 agctcaaccc cttcgacatc aatccgctgc gcgccatcgt cgccaaggtg gtggacttcg
     3301 atgccgtgca aagccggcgg gcaatccggc tgttcgtcgg tgccacccgt gtccgtaccg
     3361 gcacgctgcg gctgttcgag aaccgcgaac tgacgtccga cgcactgctg gcgtccgcct
     3421 gcctgcccac cctgcatcac gcgatcgaga tcgacggcga gccgtactgg gatggcggct
     3481 actccggaaa cccggccgtc tatccgctca tctatcgctg caatacgccg aacatcctgg
     3541 tggtcatgct gcaacctctg acccgccaag atcctccgac cagcgcacag gccattcgca
     3601 gccgcctcac cgaattccag ttcaattcct cgtttctccg cgagatgcgc agcatcgcgc
     3661 tggccaagca ggcgatcgac gcgcaatggc tgccggtcgg gcgcctggaa cgccgcttca
     3721 ggaaactgaa ttttcatctg atcgaaaccg acgacctgct cgcgcgcatg aacgtcgaca
     3781 aggcgctcaa cgccgatctc ggcttcctgc tgaaactcaa ggaagaggga cgaacccggg
     3841 cgggaacctg gttaggaaag catttcggcc aggtcggcgg ggaatcttcg atcgacttgg
     3901 taaagttgtt cggctagggc ggcgtgtcgg ttttgcagcg caaccgactc taccggtttc
     3961 ataccgacag aaagctgcgc acattatccg tctccatgtc ggcccccgcg ccgctcataa
     4021 ggacttctcc gcggcgcagt accacgtagt tgtcggcgag ttcacgtgcg aaatcgtagt
     4081 actgctccac cagcaggatg cccatgttgc cgcggtcgga gagcgcctgg atcacgcgct
     4141 cgatgtcctt gatgatggac ggctggatgc cttcggtagg ctcgtcgagg atcagcactt
     4201 tcggcttcat cgccagcgcc cggccgatcg ccaattgctg ctgctggccg ccggacagat
     4261 cgccgccgcg gcgattcctc atggtcttca gcactggaaa catctcgtag atctcgccgg
     4321 gaatgtcctt cgcggtgcca ttcttgcggc tggccaggcc catcatgaga ttttcttcca
     4381 cggtgagccg cggaaagatt tcgcgcccct gcggcacata gcccacgccc aatctcacgc
     4441 gcgcgtaagg cgggagcgtg ctcacctctt gcccgtccag tgcgatgctc ccgcttgcaa
     4501 ccggcaatgc gcccatcagg cacttgagca gcgtggtctt gcccacgccg ttacgtccga
     4561 gcagcgcggt caccgccccc gtgggcactt cgaagctcac gttgcgcagc gtctggctgc
     4621 tgccgtagaa ctggttcagt ttctcgacct tcagcatgag ttatggaaaa gtccgcttta
     4681 accacagaga cacggagaga acctgaaaac cttcaccacg aagttccggg gacaaagccc
     4741 ttgcgcgccc ttgtattgtt gacgttaact ccatatcccc tttgaccttc tctgtgcctc
     4801 tgtgtctctg tggtattcga aagcgtcaac ggcccaagta aacctcgatg acgcgctcgt
     4861 cgttcttgac ggcgtccatc gtgccttcgg cgagaacggc gccttcgtgc aacacagtga
     4921 cgacgctggc gatggagccg atgaactcca tgtcgtgctc gaccaccacc acggagcgtt
     4981 caccggccag agacttgaac agctcggcgg tgcgctcggt ttcttcgtcg gtcatgccgg
     5041 cgaccggttc gtcgaccagc agcaggcgcg gttcctgcat cagcagcatg ccgatctcca
     5101 gccattgctt ctggccgtgg gacagcaacc cggcgaggcg accggcttcg ccctg
//
LOCUS       DASQOP010000264         4269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1935863, whole genome shotgun sequence.
ACCESSION   DASQOP010000264 DASQOP010000000
VERSION     DASQOP010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1935863"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2259)
                     /locus_tag="VE030_10380"
     CDS             complement(<1..2259)
                     /locus_tag="VE030_10380"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZF23856.1"
                     /translation="MESLKVLFVEDSKADAELEVRELRRAGVKVEHRLVETEPSFLAA
                     VTEFKPDIILSDFSMPHFDGMSALTLAQSTCPLIPFLFVSGTLGEEYAIRALQKGASD
                     YVLKTNLIRLPAAVNRAVEEARGRAARRAVETELEGAHQRLNSIFESLDDAVWSWSLA
                     NQQLTYIGPAALKIFGRKPEEFSSDPALWRQIIYDADREAVLEAWRELLAGNAAFDVE
                     YRIVRTDGEIRWINDRARLIRDAGMVPERVDGIMRDITERTVARRRIDRLTRIRALSS
                     AVNSAIVRVRQREQLLEQICEIAVDVGRFRAARVVMVNDGAPPAAELVAARGGETAAF
                     ERMLADYNKAPASDEGILSRALQSRQPTFLKTEAIETASAPGDKAPSAEASQAATATL
                     PLVIKDEVVGALVLEAAEKDFSDQDEVRLLEELTANISFALELIGHQERLDYLAYYDV
                     LTGLSNRTLFHDRLSQAITAAGRENSMLALVIFNIARLRTVNESFGEHAGDSILRQVS
                     ERLKSLSHDPARVARLGGDLFALMIPGVRDLAHVTQLLAEEQFRFFDRPFVFEGRELA
                     ISARGGVALFPNDGRDAASLLHSAESALNQARESGEALLFYSTDINERVAERLKTEAK
                     LRRAVDRQEFEFHFQPKLDLHDRRTVGLEALIRWNDPETGRVVPPMEFIDLLEETGLI
                     LQVGRWALKEAVRLRAGWKEAGLKVPRIAVNISALQLKQKSFVLDVQEALAASGNDVG
                     LDLEITESMLMEN"
     gene            complement(2442..3362)
                     /locus_tag="VE030_10385"
     CDS             complement(2442..3362)
                     /locus_tag="VE030_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZF23857.1"
                     /translation="MFKLDGLTALVAVVEAGSITEAARRMGIAKSVLSERLADLERSL
                     GTRLLQRTTRKVSVTEDGSAFLERARRIVRDVSEAAAEMAERRGTLAGPLRLSAPVSF
                     GSLHLGPAIYPFLEANPGIELTLELDDRFVDVAADGYDAVVRHGPVRDNRLIVKRLAS
                     SRRMLVASPVYLARAGTPASPADLQACNAILYSNRESDWRFPGNDAANGIVIRPRKSL
                     RVNNGLIMRDAALAGLGITLLPTFLIYPELASGALRAIDIGLEAEKAEIFIAYPADRS
                     PSAKIRALTECLRSAFGDPPHWEVGLPLRQ"
     gene            3484..>4269
                     /locus_tag="VE030_10390"
     CDS             3484..>4269
                     /locus_tag="VE030_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016354381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZF23858.1"
                     /translation="MRNFLSRFILTLAGVLAATAAVTQAVADQPITIESRYADANGIK
                     LHYLVAGTGEPVLLLHGYAQTSHMWRPLMAKLAATHTVIAPDLRGFGASAKPESGYEK
                     KILAQDIRALTGSLGHQRVAIVGHDIGLMVAYAYAAQYPAEVDRIVLMDAFLPGVGDW
                     TKVWLLRDLWHFHFYGETPLALVKGRERIYFEHFWNDFAADPKRSVSERDRQIYTKAY
                     AQPGAMRAGFEVFRAFERDAADFAGFAQTKLTMPMLVLSGEKAS"
BASE COUNT          796 a         1351 c         1303 g          819 t
ORIGIN      
        1 gttctccatc agcatgcttt ccgtgatctc gagatccagg ccgacgtcgt tgccagaggc
       61 ggccagcgcc tcctgcacgt cgagaacgaa gctcttctgt ttcaactgca gcgcggaaat
      121 attgacggcg attctcggca ccttgagacc ggcttccttc cagccggccc gcaagcggac
      181 agcctccttc agtgcccagc gacccacctg caggatgagg ccggtctcct ccagcaggtc
      241 gatgaactcc atcggcggaa caacccgtcc ggtttccgga tcgttccacc gtatcaaggc
      301 ctccaggccc acggtccggc ggtcatgcaa gtccagcttg ggttgaaaat ggaactcgaa
      361 ctcctgccgg tccaccgccc ggcgcagctt cgcctctgtt ttcaaccttt cggcaacgcg
      421 ttcattgatg tccgtcgaat aaaagaggag ggcttcgccg gattcacgcg cctggttcaa
      481 tgccgattcg gcgctgtgca gaagtgaggc cgcatcgcgg ccgtcgttcg gaaagagtgc
      541 gaccccgccc cgggcggata tcgcgagttc ccgtccctcg aagacaaagg gccggtcgaa
      601 gaaccggaat tgctcttcgg cgaggagctg ggtgacgtgc gccaggtccc ggacgccggg
      661 aatcatcaat gcgaagagat ccccgcccag gcgcgccacg cgcgccggat catgggacag
      721 gcttttcagc ctttccgaga cctgccgcag tatcgaatcg ccggcgtgct cgccaaaaga
      781 ctcgttgacg gtgcgaagcc gcgcaatgtt gaaaatcacc agtgcaagca tgctgttttc
      841 gcggccggcg gccgttatgg cctgcgacag gcgatcatgg aacaaagtgc ggttcgacag
      901 cccggtaagc acgtcgtaat aggccagata gtcgaggcgc tcctggtggc cgatgagctc
      961 cagcgcgaag gaaatgttcg ccgtcaattc ctccagaagg cgaacctcgt cctggtcgga
     1021 aaaatccttc tccgcagcct ccagaacgag cgctcccacg acctcgtcct tgatgacaag
     1081 aggcagggtc gctgttgcag cctgggaagc ttccgcggac ggtgccttgt ctccgggagc
     1141 gcttgcggtt tcgatcgcct ctgttttgag gaaggtgggt tgcctggatt ggagagcccg
     1201 ggacaggatt ccctcgtcgc tcgcgggggc cttgttgtag tccgccagca tgcgttcgaa
     1261 cgcggccgtt tcgccgcctc gcgcagcgac cagttccgcg gccggcgggg ccccatcatt
     1321 gaccatgacg acccttgcgg cgcggaacct gccgacgtcg accgcgatct cgcagatctg
     1381 ctccagcagt tgttcgcgct ggcgcacccg cactatcgcg gaattgaccg cgctggagag
     1441 cgcccggatg cgggtgaggc gatctatccg ccgccgcgca accgtccgtt cggtgatgtc
     1501 gcgcatgatg ccgtcgacgc gctctggcac catgccggca tcgcgtatca ggcgtgcgcg
     1561 atcgttgatc cagcggatct ctccgtccgt cctcacgatc cggtattcga cgtcgaaggc
     1621 cgcgtttccc gccagcagtt cgcgccacgc ttcgagaacc gcttcccggt ccgcgtcata
     1681 aatgatctgg cgccacaggg ccgggtcgct cgagaactcc tccggcttgc gcccgaaaat
     1741 cttgagcgcc gcagggccga tataggtgag ctgctgattg gcgagcgacc atgaccagac
     1801 ggcgtcgtcg agggattcga aaatgctgtt gaggcgttgg tgtgcacctt ccagctcggt
     1861 ctcgacggcc cggcgtgctg cccgcccccg cgcctcttcg acggctctgt tcactgcagc
     1921 gggcaggcgg atgaggttgg ttttcaggac gtagtccgac gcccccttct gcaaggcgcg
     1981 gatggcatat tcctctccca atgtcccgga tacgaacagg aaggggatga gggggcatgt
     2041 ggactgtgcg agcgtcaggg cggacatgcc gtcgaagtgc ggcatggaaa aatccgacag
     2101 gataatgtcc ggcttgaatt cagttactgc cgccaggaat gacggctcgg tttccaccag
     2161 gcggtgttcg accttgaccc cggcgcgacg caattccctg acctcgagtt cggcgtccgc
     2221 tttggagtcc tcgacgaaca ggactttcag cgattccata aactccgctt ttacccatta
     2281 gttaaagggc actatcacta atatctagta tagtcctgtt tccgcatgta tttccatgga
     2341 aattgcaggt ttttcggctt gaattcaaga ccttcttccg agacggagaa attgggctcg
     2401 ctgagaaggc ggcggaacag cgagaagcac cacaacgcgc gtcattgtcg caacgggagg
     2461 cccacttccc agtgcggagg gtcgccgaat gcgctgcgta agcactccgt cagcgcccgg
     2521 atcttagccg acgggctgcg gtcggccggg taggcaatga agatctcggc cttctccgct
     2581 tcgaggccga tatcgatggc ccgaagcgcg ccgctcgcca gttccgggta gatcagaaac
     2641 gtcggcaaca gcgtgatgcc aagccccgct aacgcggcat cgcgcatgat cagaccgttg
     2701 ttgacccgca gggacttgcg tgggcgaatg acaatgccgt tggcggcatc gtttccgggg
     2761 aagcgccaat ccgattcgcg gttgctgtag aggatggcat tgcacgcttg gagatccgcc
     2821 ggcgatgccg gtgtcccggc ccgcgcaagg taaaccggtg acgccacgag catgcgacga
     2881 ctcgaagcca gacgtttgac gatcagccgg ttgtcccgga ccgggccgtg gcggacgacc
     2941 gcgtcgtaac cgtcagccgc gacatcaacg aagcggtcgt ccagttccaa cgtcagttcg
     3001 attcccgggt tggcctcaag aaacgggtaa atcgccggtc caaggtgcag gctgccgaaa
     3061 ctcactggcg cggatagtcg cagcggcccg gccagggtgc cgcgccgttc ggccatctcg
     3121 gcggcagcct ccgatacatc gcgaacaatg cggcgtgccc gctccaaaaa agccgatcca
     3181 tcctccgtga cggatacctt gcgcgtcgta cgctgcagca ggcgcgtgcc gagcgagcgc
     3241 tccagatcgg ccaggcgttc gctcaacacg gatttggcga tgcccatgcg tcgggcggct
     3301 tcggtgatcg atccggcttc caccactgcc accaaggcgg tcaatccatc gagcttgaac
     3361 attgttcggt atttccggaa gccagtttcg ccagcagcag gcttatccgg acgatacgcg
     3421 cagactatag tggtgtccac cggaccgatg cggatccggc gcgttcattt cttggagagt
     3481 cccatgcgca atttcttatc caggttcatc ctcacgctcg caggcgttct cgccgcgacg
     3541 gcggcagtca cgcaggccgt tgccgatcag ccaatcacga tcgagagccg ctacgcggac
     3601 gccaacggca tcaaactgca ctatctggtc gccggcacag gcgagccagt cctgttgcta
     3661 catggctacg ctcagaccag ccacatgtgg cggcccctga tggccaaact agccgccacc
     3721 catacggtaa tcgctcccga tctgcgcggc ttcggcgcgt cggccaagcc cgagtccggc
     3781 tacgaaaaga agatcctggc gcaggatatt cgcgccctca cagggtcact cggtcaccag
     3841 cgcgtggcca tcgtaggcca cgacatcgga ctgatggtgg cctacgccta cgctgcacag
     3901 taccccgcgg aagtggatcg catcgtgctg atggacgcgt ttctccccgg tgtcggcgac
     3961 tggacaaaag tgtggctgct gcgtgacctc tggcattttc atttttatgg cgagacgccg
     4021 ctcgcgctgg tcaagggccg cgaacgcatt tacttcgagc atttctggaa cgacttcgcc
     4081 gccgatccga agcgctcagt atccgagcgt gacaggcaga tctacaccaa ggcgtatgcg
     4141 cagccgggcg ccatgcgcgc aggcttcgaa gtgttccgcg cattcgaacg ggatgcggcc
     4201 gacttcgccg gcttcgccca gaccaaactg accatgccga tgctggtgct cagcggcgaa
     4261 aaggcctcc
//
LOCUS       DASQOP010000265         3659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2746081, whole genome shotgun sequence.
ACCESSION   DASQOP010000265 DASQOP010000000
VERSION     DASQOP010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2746081"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VE030_10395"
     CDS             complement(<1..547)
                     /locus_tag="VE030_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23859.1"
                     /translation="MNGRNFASLVSVAPGVSLVPSSLGQTTTGVANNPVYGNQDNYSV
                     SGSRPVGTAILLDNTDISDFFNHGTGSGVTGMALGVDAIAEFQILTNTYGAQFGGTGA
                     VLNIASRSGTNTFHGSAYEYLRNDVLDSPGYFDVDPNGKPASAPPYRRNQFGGTFGGP
                     IRKNKLFFFVNYEGLRSSLGQT"
     gene            complement(923..1678)
                     /gene="istB"
                     /locus_tag="VE030_10400"
     CDS             complement(923..1678)
                     /gene="istB"
                     /locus_tag="VE030_10400"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HZF23860.1"
                     /translation="MNLIELERSLRQLRLGGIAAVLETRLHQAQAEAMAPIDLISCLV
                     TDELTRRSERLLERRRKQAAFRDPNKTLDNFDFTFNPKMNRSLVFDLATCAFIGKRED
                     ALFLGPGGTGKSHLAQAIGQAAILQGYRVLYRETHVLLDNLADAVADGTRKEFMESLT
                     TVPLLIIDDFGMRKLPLTAAEDLLEIIMRRYERASTLLTSNRPVEDWGKLLSDVAAVS
                     AMLDRMLHHGHVLKCGPRSWRTKTAATAAGGAQ"
     gene            complement(1675..3327)
                     /gene="istA"
                     /locus_tag="VE030_10405"
     CDS             complement(1675..3327)
                     /gene="istA"
                     /locus_tag="VE030_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HZF23861.1"
                     /translation="MSNVLNEEKKQQVIALGRLGWSLRRIQRAIGVRRETAATYLKAA
                     GIAFRPPGGGGQPLPAKPAIEVITDSAAAKPALEVSTEPVPAKPASEVTTDFGAELAG
                     CTTAPPEPQPGRSPSASACGPYRDAIELALSGGRNAMAIWQDLVDAYGFAAGYQSVRR
                     FVTKLRGKQTPVACAVIETAPGEELQVDYGTGPMVREPHSGKYRRTRLFVMTLGCSRK
                     SVRLLVFRSSSQIWAELHEKAFRRLGGCTRVVVLDNLREGVLAPDIYDPSLNPLYRDV
                     LQHYGTVPLPCRVGHPDRKGKVESGVAHAQKTPLKGLRFESLEEAQTYLDHWEARWAD
                     TRIHGTTKRQVAVMFAEERPFLLPLPIEPFRYYQYGERTVHLDGCVEVEAAYYSAPPG
                     WIGRRVRVQWDGRQVRLLNPDNGQLLREHLRQARGRHRIQDEDRSKKTPPGTLQLLIR
                     AGKTGLQIGALCRGMHNQHGEIAVRRILGVLSLAKKHGAATVDDACAAALEMGVCDYH
                     FVRRYLERIPKLPLSLRQVDPLIRQLTLYRDFIENKTKESET"
BASE COUNT          667 a         1099 c         1144 g          749 t
ORIGIN      
        1 tggtctgacc gagtgaagac cgcaggcctt cgtagttcac gaagaagaat aatttgttct
       61 tcctgatcgg accgccaaag gtcccgccga attggtttcg gcgatatggc ggcgcggagg
      121 ccggctttcc attcggatcg acatcgaaat atccgggcga gtcgagtacg tcgttgcgga
      181 ggtactcgta ggcggaaccg tgaaacgtat tcgttcccga tcggctggcg atgttcagta
      241 cggcgccagt cccaccgaac tgggcgccat aagtgttagt aaggatctgg aattccgcga
      301 tggcatccac tccgagggcc atgcccgtaa cgccagaacc ggttccatga ttgaagaaat
      361 ctgaaatgtc tgtattgtcc agaaggatgg ccgtgccgac cgggcgcgac ccggaaaccg
      421 aatagttgtc ttggttgcca tagaccggat tgttagctac cccggtagtg gtctgtccca
      481 gcgatgaagg taccagcgag acaccagggg ccactgaaac cagcgatgcg aaatttctgc
      541 cattcaaacc tcggcgatca cctcaaaacc ggccactcat gatcacttca aaaccggcca
      601 ctggacacca cccaggacat gaagttgttg taccgcgccg gcggtaattc agcaagactt
      661 ttccgatttc actggtcggt ctcggcgcgt gagcttatac tgaccctacc tgggcggaag
      721 gtacggctcc gcagggatgc gaccggagcg ccgatccagc ggccggaatg gcaggggcgg
      781 cgtcagccgc ccctcaaacg agccattctg gcgcgaaagc gataaatccc aggggccatg
      841 gggacggcgt ccccatctaa gaagtgtctg cttccgattg gttttctgct ggtgccaagc
      901 ggtgcgcgtg gccggggagc agtcattggg cacctccggc cgccgttgct gccgtcttgg
      961 tacgccaact gcgcggaccg cacttgagca cgtgcccatg atgcagcatg cggtcaagca
     1021 tggcgctgac cgcagccacg tcactcaaca gcttccccca gtcttcgacg ggtcggttcg
     1081 atgtcaacag cgtactggca cgctcgtagc ggcgcatgat gatctccagc agatcttcgg
     1141 ctgcagttag tggtagcttg cgcattccga agtcatcgat gataagcagc ggcaccgtag
     1201 tcagggactc catgaactct ttgcgcgtgc cgtcggcaac tgcgtcggcc aggttatcca
     1261 gcagcacgtg cgtttcgcgg tacagcacgc ggtagccctg gaggatggca gcttggccga
     1321 tggcctgggc taagtggctc ttgcccgtgc cgcctggacc gagaaacaat gcgtcttccc
     1381 gcttgccgat gaaggcgcac gtggccagat cgaagaccag actccgattc atctttggat
     1441 tgaaggtgaa gtcgaagttg tctagcgtct tgttgggatc gcggaacgcg gcctgtttcc
     1501 gccgtcgctc tagcagtcgc tcgctgcggc gggtcagttc gtcggtgacc aggcacgaaa
     1561 tgagatcgat gggggccatg gcctcggctt gggcttggtg cagccgggtt tccagcacgg
     1621 cggcgatgcc gccaagccgg agttgtcgta aggaacgctc taattcgatg aggttcatgt
     1681 ttcactctcc ttggttttgt tttcgatgaa gtcgcggtac agggtgagtt ggcggatcag
     1741 gggatcgacc tgccgcaaac tcagtggcag ttttggaata cgctccaggt agcggcgcac
     1801 aaagtggtaa tcgcagacgc ccatttccag agcagcagcg caggcatcgt caacggttgc
     1861 ggcgccgtgc ttcttggcca gcgacagcac gcccaggatg cggcgcaccg caatctctcc
     1921 gtgctggttg tgcatcccac ggcagagcgc tccaatctgc aatccggttt tgccagcgcg
     1981 aatcagcagt tggagcgtgc ccggtggcgt tttcttggag cggtcttcgt cttggattcg
     2041 atggcggcct cgcgcctggc gcaagtgctc gcgtagcaac tgaccgttgt ccgggttcag
     2101 cagccgcacc tgccggccat cccattgcac ccggacacgc cggccgatcc agcccggcgg
     2161 cgcgctgtaa taagcggctt cgacttcgac gcagccatcc agatgtacgg tgcgctcgcc
     2221 gtactggtaa tagcggaagg gttcgatggg aagcggcagc aggaacggcc gctcttcggc
     2281 gaacatgacg gcgacctgtc gcttggtggt gccgtggatg cgcgtatcgg cccagcgcgc
     2341 ttcccagtgg tccaagtacg tctgagcttc ctcgaggctt tcgaagcgca ggcccttcag
     2401 cggcgttttc tgggcgtgag ctacgcctga ctccaccttt ccctttcgat ccggatgccc
     2461 gacgcgacaa ggcaggggca cggtgccgta atgctggagc acatcgcggt acagcgggtt
     2521 gagcgaggga tcgtagatgt cgggggcgag cacaccctcg cgcagattat ccagcacaac
     2581 gacgcgtgtg cagccaccca gcctgcggaa ggccttctca tgcagttcgg cccagatctg
     2641 cgagctggac cggaacacca gcaagcgaac cgacttgcga ctgcaaccga gagtcatcac
     2701 gaacagccgc gtgcgccggt acttgccgct gtgcggttca cgtaccatcg gtccggtgcc
     2761 gtaatccacc tgcagctctt caccaggtgc ggtctcgatc actgcgcagg ctaccggtgt
     2821 ctgcttaccg cggagcttgg tgacgaagcg cctgacgctc tggtaaccgg cggcgaaacc
     2881 gtaggcgtcc accagatcct gccagatcgc catggcgttg cgaccacccg agagcgcgag
     2941 ttcgatcgcg tctcggtagg gcccacaggc gctggccgat ggactgcggc cgggctgcgg
     3001 ttcgggaggc gcggttgtgc agccggccaa ctccgcgcca aagtcggtgg tcacctcact
     3061 ggccggtttt gcggggaccg gctcggtgct gacctccagg gcgggtttcg ccgccgcaga
     3121 gtcggtgatc acctcgatgg ccggttttgc cggtagaggc tgccccccac cgccgggcgg
     3181 ccgaaaggca attccagcgg cctttaaata ggtagccgcc gtttcgcggc gaacaccgat
     3241 ggcccgctgg atgcgccgca gcgaccatcc caaccgcccc aaagctatga cttgctgttt
     3301 cttttcctcg ttcaagacgt tgctcacgtt gacggagggt atcgccctcg cgtcgagtca
     3361 acgtcctggg tgatggccta tgggtggccg gttttcaggt gatcatgagt ggccggtttt
     3421 gggtgttcac cgaggctgtt gagcgtggag gctctcaaca gctacaaaat catctacagt
     3481 ggcttgcgcc gcgtgatgtc ccttatgtcg tccctgggac cttacccgcc gaaccccgcc
     3541 gcggtttacg cgtccgcgtc gttgtcgagc tcgccttcca gttcgtaccc gatggcgcga
     3601 tatcccttga gccgcttctc gtacatccga gcaagcattg gaacgtgtac gtccacgta
//
LOCUS       DASQOP010000266         9705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_532192, whole genome shotgun sequence.
ACCESSION   DASQOP010000266 DASQOP010000000
VERSION     DASQOP010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9705
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532192"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..61
                     /gene="pqqA"
                     /locus_tag="VE030_10410"
     CDS             <1..61
                     /gene="pqqA"
                     /locus_tag="VE030_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HZF23862.1"
                     /translation="PEYTEMRFGFEVTMYGFNR"
     gene            complement(706..3006)
                     /locus_tag="VE030_10415"
     CDS             complement(706..3006)
                     /locus_tag="VE030_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZF23863.1"
                     /translation="MAVRRHPTPRARLAFAVTLALSGSMAHGAEENPATALEAPTVEI
                     IGTTPLPGIGVPVNQVPANVQAATGAEIQKQKTLDLSEFLDNNLGSVTLNHGQNNPFQ
                     PDVNFRGLSASPLLGTPQGLSVFMDGVRINEPFGDVVNWDLIPQNAISTITLIPGSNP
                     VFGLNTLGGALSVNTKSGFQYPGASASIYGGSWGRKAAEFEYGMHGEKADLFLAGNLL
                     DEDGWREHSPSRIRTLFTKVGLEREDTDLDVSLMLADNKLEGTQALPLPWLDARKQAY
                     TWPDRNENQLAFLNVKASHFLAADKLLAGNLYYRRYTNDNFSSNVNDDCKGIVGNCAT
                     GVLAGGADPQALNDLSKIETTGYGGSVQLTLLGDLRGKKNNFTVGASADLGRTDFSQS
                     EEAADFTADRGTVGTGVFSLATDVKTTNDYYGLYLTDTYSFTERWHLTLSGRYNWAKV
                     KIEDKTGLEPALNGDHTFTRFNPAAGLNFNPTPELSTYVAYSEGMRAPTPIELTCADP
                     AAPCRLPNNFLADPPLEKVVSKTMEAGLRGRWTEALGWNASVYRTDLNDDIIFISSGG
                     AVNAGFFQNAGKTRRQGLELGMHSRIGKLNLAANYGYIDATFESALTLNSPANSSAVS
                     NDIQVSPGSKVPGIPEHSLKLRMEYDFTEAVSVGTNIMYFSSQYARGDENNQDANGKL
                     PGYALVHLDARYQLNKKLMFFGRVTNLFDKEYETLGVLGENFFNGPGRTFNASPGNVT
                     DEQFRSVGAPRGIWVGVKYDLGKPGN"
     gene            3348..4676
                     /locus_tag="VE030_10420"
     CDS             3348..4676
                     /locus_tag="VE030_10420"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZF23864.1"
                     /translation="MNTLAQTFQAPPANIIKPSVHRDLVVVGAVTVLTFVMSSLFELN
                     EWIEALTVPHERYQIDELPLTILAMAVALAWFSWRRSRQTVEQVTLRLAAQQALMDSE
                     EQYRTLFMENLSGNLLATTTTGQIKLANPMAARLLGFETAGELIGHRLGEFYAECDLW
                     ETHCDRLLRGDKIELPMLQLRNRNGAPVQVVAKLSARLSPSRESELHVYVTDITDVTL
                     MQNKLAGALEENRRLSQRSMQVQEEERRNLARELHDELGQSLNAIKVDAVTIRDNSAA
                     LPEIHRGALSIIEVSSQVYDVVRSLMQRLRPVALDELGLRSAMQYSVEQWQRRHAGVR
                     CTFTADGELDDLDEQTNITLYRLVQECLTNVAKHADATQVGISMTREAHQLRFSFGDN
                     GKGFDPSQKRRGLGLVGLRERVEALAGHFELDSTPGRGVQVKAVIPAKGK"
     gene            4676..5368
                     /locus_tag="VE030_10425"
     CDS             4676..5368
                     /locus_tag="VE030_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZF23865.1"
                     /translation="MNEAGTSPLVRVALVDDHAVVREGYRRLLEHTEDISVIAEVASG
                     EDAYRVVCEMQPDVTVMDINLPGLGGIETVRRIVARLPEAKILMFSMHEDTVFSSRAL
                     QAGARGYVTKSAAPEVLVEAVRLVAAGKLYISHVMAQELALQMLPGRDNPIDALSARE
                     FEVFRLLVAGHTLQEISRILCLSYKTVANYQSNMKHKLDVSNTAQVVRIALSHGLIAG
                     AAEAEENRSESG"
     gene            5629..6576
                     /locus_tag="VE030_10430"
     CDS             5629..6576
                     /locus_tag="VE030_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HZF23866.1"
                     /translation="MTNRYLQAAAGLLVTAATVTSAAPFAYVANEESGTVSVIDTATD
                     KVVRDIPAGKKPRGMALSKDGKHLYVSDQPKNVLDIIDVEKGEVVGTVALGESPEGVG
                     ISPDGMWVVAAVEVSNSIAFINTRTNQREFLVKVNGKNPEHAVFSPDGKWVYSSAEEA
                     SQIDVIDVAQRKQVTEIPVGKRPRGIAFLPDGKVAYAASEYASTVHALDVTKHALIAT
                     IPGGQFSNGVTVHPNGSRIYISNGKDATVSVIDTATNKIIATIEVGKRPWNMAITPDG
                     GKLYVANGRSDSVSVIDTKTNTKIKDIPVGTRPWGVVIQ"
     gene            6641..7186
                     /locus_tag="VE030_10435"
     CDS             6641..7186
                     /locus_tag="VE030_10435"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HZF23867.1"
                     /translation="MNNNVVQRYTTPAITLHWIIAILVLVMIGLGLYMTEIPRGTPER
                     TFYYNLHKSIGVTVAVLVLIRLWWRFRNPPPPLPSSVPAWQVQASKLSHALLYLCLIL
                     MPLSGFSASQFTKYGVTYFGLFKIPPMGWEDKVIYDFLQGIHGVTAVVLITLVVIHVA
                     AALKHLLFDRDNVFLRMLPGK"
     gene            complement(7290..8963)
                     /locus_tag="VE030_10440"
     CDS             complement(7290..8963)
                     /locus_tag="VE030_10440"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HZF23868.1"
                     /translation="MASAANLATLIVDKGDSAKVSQGARNLQRGLIKAFRIQIAPVRA
                     PDLGNAAALYQSICAACHGAAGDGNGPQAAALEPRPTNFRDHARQFERSLYGLYNTIT
                     FGVEGTSMQAFASLRAEERWALAFFVSQFVSTEDERNQGGQLWERPGMRTLFPGLSDL
                     VGATPAETLRVGTSAYQVFAYLRAHPAMIPSQDSTAPLSIATAKIRESLERYRQGNRE
                     AALQAAISAYLEGYALAEAGLDRVDHSLRNRIEQDMMAYRSLLRNTAAVDVVEHRALA
                     LTKSIEEAATLSSRGELSAAGAFTAAFIIIAREGLQAILVLAAVAAFLIKSGRRQGLK
                     HLHAGWVVALLLGVATWIASSTLVEISGAQREFTEGLTALLFAVVLLYAGYRLHGRSH
                     ASRWHSSIRGQASDSLSQATLCGIALVSFLAVFGEAFETVPFMQGLWMLSDRVWRGGL
                     VAGVASAGAALAIIAWLVCRYSARLPWWLLFSLSSLYLAVLAVIFAGKGVAALQAAGQ
                     LPTDPLPISGIPALGIYPSVQGLALQSLLVVVIVASFLYGRVDDRPRSS"
     gene            9033..9176
                     /locus_tag="VE030_10445"
     CDS             9033..9176
                     /locus_tag="VE030_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23869.1"
                     /translation="MLGVLIGVDNFAGLDELGILDRNGVEQAQDHLTLGGVQILSLRR
                     DRK"
     gene            9403..>9705
                     /locus_tag="VE030_10450"
     CDS             9403..>9705
                     /locus_tag="VE030_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZF23870.1"
                     /translation="MSFKITLMSLALAMFAGCAVVPSAPPVVAPVAEEVTSPEPPPPP
                     KIALVLGGGAAKGFAHIGVIKALESHAITPDIIVGTSAGSVVGALYAGGYNGFDLQR"
BASE COUNT         1955 a         2831 c         3026 g         1893 t
ORIGIN      
        1 acccgaatac acagaaatgc gctttggctt tgaagtcacg atgtacggct tcaatcgctg
       61 atagcactgc aagtcccgaa gggctgcttt cgagcagcct tttttttgcg tcaaggcggc
      121 gcgggagcgc cccaagaaaa aagagaaaac tggatcaaaa aaaatcaagg gagaacgtgc
      181 cttgcaattt caggaccctc aatcttccgg tcaggcaaat tttctgcctc gcactgtcat
      241 ttttcgatac cggcgctgtc ctggcgcagg aagactcagt tcagttggca cccatcgtgg
      301 taacgcccac acgggttgag cagtcgagct tcgatctgcc ggtttcgcag agcttggctg
      361 ggcttacaac ccgttgggat tctcgactgc ggtggaagcg cgctgggtcg acaaggtatt
      421 taccaatgat ttgaatgacg aatctgcgga cgcttacaca ttggtcaacg tgcggttcgg
      481 cttcgagcag aagctcggcg ggtggaagct caatagaatt ggcccgcgtc gacaatattt
      541 ttgacgaaga atacgtgggg tcggtaattg taaacgcttc gaatggacaa tattacgaac
      601 ccgcgcccgg gacgaactat gcggtaggcc ttgccgccag ctatcaattc tgaagaaagg
      661 gtcagaaaat aagggggagc tatcgctccc tctgttcgag cggtctcagt tgccaggctt
      721 gccgaggtcg tacttgaccc cgacccagat tccgcgcggt gcgcccaccg agcggaactg
      781 ttcatcggtc acattgccgg gactggcatt gaacgttctg ccgggaccgt tgaagaaatt
      841 ttctcccagt acgcccagcg tttcgtactc tttgtcgaac agattggtga cgcggccgaa
      901 gaacattagc ttcttgttga gctggtaccg tgcatccaga tgcaccagcg catagccggg
      961 aagttttccg ttggcgtcct gattgttttc gtcgccgcgc gcatactggg acgagaagta
     1021 catgatgttg gttcccactg atacagcttc ggtgaagtcg tactccatgc gcagcttcag
     1081 cgagtgctcg ggaatgcccg gcaccttgct tccgggtgaa acctggatat cgttggaaac
     1141 cgcggaagaa tttgcagggc tattgagcgt cagcgccgat tcgaacgtgg cgtcgatata
     1201 accgtaattc gccgccaggt tcagcttgcc aatgcggctg tgcattccga gttccaagcc
     1261 ctgccggcgc gtcttgccgg cattctggaa aaagcccgca tttaccgccc cgccgctgga
     1321 aatgaagata atgtcgtcat tcaggtcagt ccggtaaacg gaggcgttcc agcccagcgc
     1381 ctcggtccag cggccacgca acccggcttc cattgtcttc gaaacgactt tttccagagg
     1441 gggatcggcc aggaaattgt tggggaggcg acacggtgcg gcgggatcag cacaagtaag
     1501 ctcgatcggg gtcggtgcgc gcatgccctc gctgtatgcc acgtaagtcg agagttccgg
     1561 tgtcgggttg aaattcagac ccgctgccgg attgaaacgt gtgaatgtgt gatcgccatt
     1621 gagcgccggc tccagaccgg tcttgtcctc gatctttact ttcgcccagt tgtagcggcc
     1681 cgacaaagtc aggtgccatc gctcggtgaa tgagtaggtg tcggtgagat aaaggccgta
     1741 gtagtcgttg gttgtcttga catcggtcgc gagggaaaac acgccggtgc cgactgttcc
     1801 gcgatcagcg gtgaagtccg cagcttcctc cgactgggaa aagtcggtcc tgcccagatc
     1861 ggcgctggcg ccgaccgtga aattattctt ctttccgcgg agatcgccca agagtgtcaa
     1921 ctgcaccgag ccgccgtaac cggtggtctc gatcttgctg agatcattga gggcctgcgg
     1981 atcggcaccg cctgcgagta cgccggttgc acaattaccg acgatgccct tgcaatcgtc
     2041 attgacgttg ctgctgaagt tatcgttggt ataacgccgg tagtagaggt tgccggcaag
     2101 cagcttgtcc gccgccaaga agtggctggc tttcacattc aggaacgcca actggttctc
     2161 attgcggtcc ggccaggtat aggcctgctt gcgcgcatcc agccagggca gcggcaatgc
     2221 ctgcgtgccc tccagtttgt tgtcggccag catgagggac acatcgaggt cggtgtcttc
     2281 ccgctccagg cccaccttgg tgaacagcgt ccgtatccgg ctcggcgagt gttcgcgcca
     2341 gccatcttcg tcgaggaggt tgccggcaag aaacaggtcc gccttttcgc catgcatgcc
     2401 gtactcgaac tcggccgctt tgcgacccca cgacccgcca tagatcgacg cggacgcccc
     2461 cgggtactgg aagcccgact tggtgttcac cgagagcgct cctcccagcg tattcaagcc
     2521 gaacaccgga ttcgagccgg ggatgagggt aatagtggaa atcgcattct gcgggatgag
     2581 atcccaattc accacgtcgc cgaacggttc gtttatccgt accccgtcca tgaacaccga
     2641 cagtccctgg ggcgtcccca gcagcggcga ggcggacagc ccgcggaaat tgacgtcggg
     2701 ctggaatggg ttgttctgcc catgattgag ggtgacgctc ccgaggttgt tgtccaggaa
     2761 ttcggagagg tccagagttt tctgtttctg gatttcggca ccggtagcgg cctgcacgtt
     2821 ggcgggcacc tggttgacgg gcaccccgat cccgggaaga ggggtggtgc caataatttc
     2881 cacggtcggc gcttcgagtg cggtggcggg attctcttcg gcgccgtggg ccatactgcc
     2941 ggacagtgcc agagtgacgg cgaaggcgag gcgcgcccga ggagtcgggt gtcgtcgaac
     3001 ggccatctat gttcctttcg gcttttctac ttgattatgg agccggtaca aactcgctcc
     3061 cctgccttgg tagccagggc cggcgccgtc aatcggcgcg cgggcctgct ccaaacagca
     3121 ggagggagaa tctaaggggt gcggcagact gcctggcagg gactgcctcc caaccggatt
     3181 gggaaaaatt cccaatctcc gggctggctt tcaggattcc cggcgagact agcgcaatat
     3241 gcgccccccg ggagaatttt cctttgcgag ggctggcgcg ttccgctata tagactccct
     3301 acctatccaa ttccccgccg gcccggcgac ctccgactgt aacttccatg aatacactag
     3361 cccagacgtt tcaggcgcca cccgccaaca tcatcaagcc ctcggtgcac agggacctgg
     3421 tggtggtcgg cgcggtcacc gtgctcactt tcgttatgtc cagcctgttc gagctcaacg
     3481 agtggatcga agcactgacg gtgccccacg agaggtacca gatcgacgaa ctgccgttga
     3541 cgatactggc catggcagtg gcgctggcgt ggttttcctg gcggcgttcc aggcagacgg
     3601 tcgagcaggt gacgctccgt ctagccgcgc agcaggcttt gatggacagt gaagagcagt
     3661 accgcacgct gttcatggaa aacctgtccg gcaatctgct ggcgacgacg acgaccggac
     3721 agataaaact cgccaacccg atggcagcgc gactgcttgg ctttgaaact gccggcgaac
     3781 tcatcgggca ccggctgggt gagttctatg ccgagtgcga tctgtgggag acgcattgcg
     3841 accggctgct gcgcggcgac aagatcgaat tgccgatgct gcagctcagg aatcgcaacg
     3901 gcgcgcctgt gcaggtcgtg gcaaagctga gcgcacggct gtcgccctcc cgggaatcgg
     3961 agctgcacgt ttacgtcacc gacatcaccg acgtcacgct gatgcagaac aagctggctg
     4021 gcgcgctgga ggaaaaccgc cgcctgtcgc agcgttccat gcaggtgcag gaagaagagc
     4081 ggcgcaacct ggcgcgcgag ttgcacgacg aactggggca aagtcttaac gcgatcaagg
     4141 tagatgcggt cactatccgc gacaacagcg cggcactccc cgagatacac cgcggcgcgc
     4201 tttccatcat cgaagtgtcg agccaggtct acgacgtagt gcgcagcctc atgcagcggc
     4261 tgcgcccggt ggcgctggac gaactcgggc tgcgctccgc catgcagtac agcgtcgaac
     4321 agtggcagcg ccgccacgcc ggcgtgcgct gcacgttcac cgcggacgga gaactcgacg
     4381 atctcgacga gcagaccaac atcacgcttt accgcctggt ccaggaatgc ctgaccaatg
     4441 tggccaagca cgccgatgcg acgcaggtcg gtatatccat gacccgcgaa gcgcaccaat
     4501 tgcgcttcag tttcggcgac aacggtaagg gtttcgaccc gtcgcagaaa cgccggggac
     4561 tggggctggt ggggttgcgc gaaagagtgg aagcgctggc cggccatttt gaactcgaca
     4621 gcacgcccgg ccggggcgtg caagtgaaag ccgtgattcc ggcgaaggga aagtgatgaa
     4681 cgaagcaggg acaagcccgt tggtgcgggt ggcgctggtc gatgatcacg ccgttgtgcg
     4741 cgaaggctac cgccgattgc tggagcacac cgaggacatc tcggtgattg ccgaggtcgc
     4801 cagcggcgag gacgcttacc gcgtcgtgtg cgaaatgcag ccggatgtca ccgtgatgga
     4861 catcaacctg cccggactcg gcggcatcga gacggtgcga cgcattgtcg cgcggctgcc
     4921 ggaagcgaag attctgatgt tcagcatgca cgaggacacg gtgttctcct cgcgcgcgct
     4981 gcaggccgga gcgcgcggtt acgtcaccaa gtccgccgcg cccgaagtgc tggtggaggc
     5041 tgtgcggctg gttgccgccg gcaagctcta cattagccac gtaatggcgc aggaactggc
     5101 gctgcagatg cttcccgggc gcgacaaccc gatcgacgcg ctgtccgcgc gcgagttcga
     5161 agtgttcagg ctgctcgtgg cggggcacac gctgcaggaa atttcccgca ttctgtgtct
     5221 gtcctacaag accgtcgcca actaccagtc caacatgaag cacaaactcg acgtgagcaa
     5281 taccgcgcag gtggtacgca tcgcgttgag ccatgggctg atcgcaggcg cggctgaagc
     5341 cgaggagaac cgcagcgaat cgggttgaat gccggtcggg aatcgtagcg ccacctgtcc
     5401 cgaaccgcct gtcatcggtg taccagggag ccgcgttatg cgacgttacg tgatggtgat
     5461 agcatgtcgc cgcgccaacg ggtggtgacg attcaggcat tgccggattc gggagtattt
     5521 tccaagaact tttcgatgcg ggcgcggctc caagcagcgt cagggggggt gagcatctct
     5581 tgaacgcgcg cgttgacaat ctgatccatt aatcggggaa aaatcaggat gactaacaga
     5641 tatctccagg ccgcggcagg acttctggtg accgcggcaa cggtgacaag tgcggcacct
     5701 tttgcctatg tggcgaacga ggaaagcggg acagtgagcg tcatcgatac cgcaaccgac
     5761 aaggtagtcc gggacatccc ggcaggcaag aaaccgcgcg gaatggcgct gtcgaaggat
     5821 ggcaagcacc tttacgtcag cgaccagccc aagaatgttc tcgatatcat cgatgtcgaa
     5881 aagggcgagg tcgtcggcac ggtggcactg ggcgaatccc cggagggtgt cggcatatcc
     5941 ccggacggca tgtgggtggt cgctgcggtg gaggtgagca actcgattgc cttcatcaac
     6001 accaggacca accagcgcga gtttctggtg aaggtaaatg gcaagaatcc ggagcatgcg
     6061 gtattcagtc ctgacggcaa gtgggtttat tccagcgccg aggaggcctc gcagatcgat
     6121 gtcatcgacg tcgctcagcg caaacaggtc acagaaattc ccgtgggcaa acgtccgcgt
     6181 ggcattgctt tcctgcctga cggcaaggta gcttatgccg cgtccgagta tgccagcacc
     6241 gtccatgcgc tcgacgtgac caaacacgcg ctcattgcca ccattcccgg tgggcagttt
     6301 tccaatggcg tgacggtcca tcccaacggc agccgcatct acatttccaa cggcaaggac
     6361 gcgacagtgt ctgtcatcga caccgcaacc aacaagatca ttgccaccat cgaggtggga
     6421 aagcgcccct ggaacatggc catcacgccg gacggaggca aactttatgt cgccaacggc
     6481 cgctccgatt cggtgtccgt gatcgacacg aagaccaaca cgaagatcaa ggatattccg
     6541 gtgggtaccc ggccctgggg ggttgttatc caatagcagc cgcgtaacat acgcgcgtcg
     6601 atgatgcgcg tatcagattc aaaggggagg atcttcggac atgaataaca acgttgttca
     6661 acgctacact acgcccgcga tcacgctgca ctggatcata gcgattctcg ttttggtgat
     6721 gatcgggctc ggcttgtaca tgaccgagat cccgcgcggc acgcccgagc gcactttcta
     6781 ctacaacctg cacaagtcga tcggtgtgac ggtcgcagtc ctggtactga tccgtctatg
     6841 gtggcgcttc cggaatccgc cgccgccgct gccctcgagc gtaccggcct ggcaggtgca
     6901 ggcatcgaag ctgagccatg cgttgttgta tctgtgcctg atcctgatgc ctttgtccgg
     6961 attctcggcg tcccagttca ccaagtacgg cgtaacgtat ttcgggttgt tcaaaatccc
     7021 gccaatgggg tgggaggaca aggttatcta cgattttctg cagggcatac acggcgtaac
     7081 cgcggtggtt ctgatcacgc tggtggttat tcacgttgcg gcggcgctga agcatctgct
     7141 gttcgatcgt gacaacgtgt tcctccgcat gttgcccgga aagtagaagc cgtcttcgga
     7201 atttgcgcaa ccccagccgt aaggcggggc ctttcgttag cgcaacgcga ccacgtgatc
     7261 ggtctccagc cggttcctcg ccccattcgt catgatgaac ggggtctgtc atcgacgcgg
     7321 ccgtaaagaa aactggcgac gatcacgacc acgagcagcg actgcaacgc cagaccctga
     7381 acgcttggat agatgccgag cgcagggatg ccggagatcg gcagcggatc ggtcgggagc
     7441 tggccggccg cttgcagcgc cgccaccccc ttgccggcga agatcactgc cagcacagcc
     7501 agatacagcg agctgaggct gaagagcaac caccaaggca gcctggcgct gtagcggcac
     7561 accagccagg caatgatggc aagcgcggcg ccggcactcg cgacgccggc aaccagtccg
     7621 ccgcgccaga cgcgatccga cagcatccac agcccctgca tgaacggcac ggtctcgaag
     7681 gcctccccaa agacggcgag aaatgaaacc agcgcgatac cacacagcgt tgcttgcgac
     7741 aacgagtcgc tggcctgtcc gcgaattgac gaatgccatc gcgatgcatg cgaacggccg
     7801 tggagccggt agccggcgta caacagcacg acggcgaata gcaatgcggt caggccttcg
     7861 gtaaattccc gctgtgcgcc gctgatctcc acgagcgtcg aggatgcgat ccaggtggcc
     7921 acgccaagca gcagcgcgac aacccacccg gcgtgtaggt gtttgaggcc ttgcctgcgc
     7981 ccggacttga tcaggaatgc cgcgacggcc gcgagcacga gaattgcctg caggccttcg
     8041 cgcgcgatga tgatgaaggc tgcggtgaag gcgccggccg cggatagttc gccgcgggag
     8101 ctgagggtcg ccgcctcttc gatggatttc gtgagggcaa gtgctcgatg ctcaaccaca
     8161 tccacggcgg ctgtgttcct gagcaggctg cggtaggcca tcatgtcctg ctcgatgcgg
     8221 ttgcgcagac tgtggtcgac ccggtcaagg ccggcctcgg cgagtgcgta gccttccagg
     8281 taggcgctga tcgcggcctg gagggcggcc tcgcggtttc cctgtcggta gcgttcgagg
     8341 ctctcccgga ttttggcggt cgcaatcgac agcggggcag tgctgtcttg cgagggaatc
     8401 attgcgggat gcgcgcgcag gtatgcgaac acctgatagg cggacgtgcc gacgcgaagc
     8461 gtttccgcgg gagtggcacc gacgaggtcc gacaggccgg gaaacagcgt tcgcatgcct
     8521 gggcgctccc agagctggcc gccctggttg cgttcgtctt cagtggagac gaactgactt
     8581 acaaagaacg caagcgccca gcgttcttcc gcgcgcaggc tggcgaatgc ctgcatcgag
     8641 gtgccttcga cgccaaaggt gatggtgttg tacaagccgt aaaggctgcg ctcaaactgg
     8701 cgggcatgat cccgaaaatt cgtcggccgc ggctcgagtg cggcagcctg cggaccgttg
     8761 ccatcgcccg cggcaccgtg gcaggccgcg cagatcgact ggtagagtgc agccgcattt
     8821 cccagatcgg gtgcgcgcac aggggcaatc tgaattcgga aagccttgat caggccacgc
     8881 tgcaagttgc gcgcgccttg cgagaccttg gcgctgtcgc ctttgtcgac gatcagcgtg
     8941 gctaggtttg ccgccgatgc caccagagcg tccttttccg ggcgtgccgg gaaattcctg
     9001 acggcgtctg cgacctgagc ggagaactcg atttgctcgg cgtactcatc ggcgttgaca
     9061 actttgccgg acttgacgaa ctggggatac tcgacagaaa tggagtcgag caggcgcaag
     9121 accatctgac cctgggcggg gtccagatcc tgagcttgcg acgtgaccgc aaatagaatc
     9181 gccagaaaaa gcgcgacaca gacgcggcgc agtgaagcaa acatcacggg gtagtcaggc
     9241 cggaagcatg taacggcaat gatacgtata tgatccgcgc agaaccgtgc attctgtttt
     9301 attgacaatc attctcattg tgattaaatc gtgagcgaag gcacgctgaa ccctctcccg
     9361 tttccttcct gccccgctgt ttggcggaag aaatgcggtc cgatgtcatt caaaatcacg
     9421 ctgatgtccc tggcacttgc gatgttcgcc ggttgcgcgg tcgtgccttc agcgcctcct
     9481 gtcgttgcac ccgtcgcgga ggaagtcacc tcgccggagc cgcccccgcc gccaaagatc
     9541 gcgctcgtac tgggcggcgg ggccgccaag ggcttcgccc acatcggtgt catcaaggca
     9601 ctcgaatcgc acgcgatcac gcccgacatc atcgtcggta ccagtgccgg gagcgtcgtc
     9661 ggggcgcttt atgccggcgg ttacaacgga ttcgatttgc agcgt
//
LOCUS       DASQOP010000267         3679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2144808, whole genome shotgun sequence.
ACCESSION   DASQOP010000267 DASQOP010000000
VERSION     DASQOP010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2144808"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1190)
                     /locus_tag="VE030_10455"
     CDS             complement(<1..1190)
                     /locus_tag="VE030_10455"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZF23871.1"
                     /translation="MDAQTATKPELLIVDDDPLITDTLNFVLGKDYSVRVAASRSQVK
                     SLLRQLDAPPVLALVDLGLPPTPHRPDEGFRLITELLAYSPSIKILVLSGQNDEGNAR
                     YARTLGAVEFVAKPCEPEVLKTMLAKALQIAEVEQSSADLDTGIVGSGFAIRKLHQQI
                     LQYAPAPFPVLIEGESGSGKELVAQSLHRQSQRGARPYLALNCAAISPTLVEPTLFGY
                     VKGAFTGATSSRSGYFEDAHEGTLFLDEIGELPLELQAKLLRVLENGEFQRVGETQSK
                     TSNARVVAATNRDLRQEVRAGRFRADLYHRLSVFSIAVPPLRELSEDKLRLLQHYSAI
                     YALQSGMQPFQLDPKSMDLWMRYGFPGNVRELRNVVIRLTTKYAGQTVNSEQLLAEFD
                     WEEPA"
     gene            complement(1276..2010)
                     /locus_tag="VE030_10460"
     CDS             complement(1276..2010)
                     /locus_tag="VE030_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZF23872.1"
                     /translation="MTMDGAGDISAISFRGLRKSYADTAVLHDLELDVPAGRTFGLVG
                     MNGAGKTTLIKCLLDFCEFDAGDIRIFSLPSQDTHARARLAFLPERFVPPYYLNGEDF
                     LRYMMKLYSCGYEPGQVRRMLAELDLAESALRKPVRTFSKGMTQKLGLAACLLSNREL
                     LVLDEPTSGLDPKARALLKVRLQTAHAAGRTVFFTSHALADVDELCDRMAVLHEGQLR
                     FSGTPLELRTRYSAATLEQAYLACIQ"
     gene            2129..2941
                     /locus_tag="VE030_10465"
     CDS             2129..2941
                     /locus_tag="VE030_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZF23873.1"
                     /translation="MMAHSDNLVEISGLNFWYDKRRPILSGIDMAIPRGKVVSIMGIS
                     GSGKTTLLRLIGGALKPRQGQVKVAGKVMHQLDRDGLYEMRRRMGMLFQFGALFTDLS
                     VFDNVAFQMREHTDLPESMIRDMVLMKLHAVGLRGAHRLLPSELSGGMARRVALARAI
                     ALDPMLIMYDEPFAGLDPISLGVIGNLIRRLNDALGATSIVVTHDVQESLKMVDYVYY
                     LSEGLIVAKGTPKEMQASTEPLVHQFVYGEPDGPVPFQYPSADYGDDLMLNR"
     gene            2943..>3679
                     /gene="mlaE"
                     /locus_tag="VE030_10470"
     CDS             2943..>3679
                     /gene="mlaE"
                     /locus_tag="VE030_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HZF23874.1"
                     /translation="MFNRSAIVNVRSIGQHTVNGLWRLGYASRFFALILLQSGTSFRR
                     LRLIIRELYFTGVLSLIIVLVSGLFVGMVLGLQGYQTLQTYGSESALGVLVALSLVRE
                     LGPVVSALLFASRAGSAMTAEIGLMKATEQLSAMEMMAVDPIARVVAPRFWAGVISMP
                     LLAAMFSAMGVFGGYLVGVVLIGVDEGSFWSQMQNAVDFREDIVNGVIKSFVFGVAVT
                     WIALFEGYDAPPTAEGVSGATTRTVVTS"
BASE COUNT          663 a         1092 c         1172 g          752 t
ORIGIN      
        1 gccggctcct cccagtcgaa ttctgccaac aactgttcgc tgttgaccgt ctggccggcg
       61 tatttggtgg tcaggcggat gacgacattg cgcagttcgc ggacattgcc cgggaaacca
      121 tatcgcatcc acaggtccat agacttgggg tccagctgga aaggctgcat tccgctctgg
      181 agcgcataaa tcgccgagta atgctggagc agcctgagct tgtcctcgct tagttcgcgc
      241 aacggcggca cggctatcga gaacaccgac aagcggtgat aaaggtcggc gcggaaacgc
      301 ccggcgcgca cctcctggcg caggtcgcga ttggtggcgg cgacgactcg ggcattggac
      361 gtcttgcttt gggtttcgcc gacgcgctga aactcgccgt tttccagaac tctaagcagc
      421 ttggcctgga gctcgagtgg caactcgccg atctcgtcca gaaacagcgt gccctcgtgg
      481 gcatcttcga aataaccgga tcgcgatgac gtggcgccgg tgaacgcgcc ctttacatag
      541 ccgaacagcg tcggttcgac cagtgtcgga gaaatcgctg cacagttgag cgcgagataa
      601 ggtctggcgc cgcgttggct ctgccggtgc agcgactgcg ccaccaactc cttgccgctg
      661 cctgattcgc cttcgatcaa caccggaaaa ggtgccggcg cgtactgcag gatctgctga
      721 tgcagtttcc tgatggcaaa accgctgccg acgatgccgg tgtcgagatc cgcggagctc
      781 tgctcaacct cggcgatctg cagcgccttg gcgagcatgg tcttgaggac ctccggttcg
      841 cagggcttgg caacgaactc gacggcgccg agagtgcgcg cgtaacgggc attgccttcg
      901 tcgttttgac ccgacagcac cagtatcttg atcgagggcg agtaggcgag cagttcggtg
      961 atcaggcgga agccttcatc cgggcggtgc ggggttggcg gcagtcccag atcgacgagc
     1021 gccagcactg gcggcgcgtc gagctggcgc aacaggcttt tcacctgcga tcgcgacgcc
     1081 gcgacgcgca cgctgtagtc cttgcccaat acgaagttga gcgtgtcggt gatgagcgga
     1141 tcgtcgtcga cgatcagcag ttcgggtttg gtcgctgtct gggcgtccat gttgccggca
     1201 ggcacggcgg gattcggttc atttgtttga ttgtgccaga aaagttagac ggctcataga
     1261 gttagccatg tcaggtcact ggatacacgc cagataggcc tgctccaaag tagcggcgct
     1321 gtagcgggta cgcagctcca aaggcgtgcc ggagaagcgc agttgtccct cgtgcaatac
     1381 cgccatccga tcgcacagtt catcaacatc ggcgagcgcg tgcgaggtga agaacacggt
     1441 gcggccggct gcgtgcgccg tctgcagccg gactttcagc aacgcgcggg ccttggggtc
     1501 cagcccgctg gtcggttcgt ccagcaccag caactcgcga ttcgagagca ggcaagcggc
     1561 gagcccgagc ttttgcgtca tccctttgga gaaggtgcgc accggcttgc gcagcgccga
     1621 ctcggcgagg tcgagttcgg cgagcatgcg ccgcacttgc cccggctcgt agccgcatga
     1681 atacagcttc atcatgtagc gcaggaaatc ctccccgttc aggtagtagg gaggcacgaa
     1741 ccgctcgggc agaaacgcca ggcgcgcccg ggcgtgggta tcctgactcg gcaaactgaa
     1801 aatacggatg tcccccgcat cgaattcgca gaaatccagc aggcatttga tgagcgtggt
     1861 cttgcccgcg ccgttcatgc cgaccagccc aaaagtgcgg ccggcgggga cgtcgagctc
     1921 cagatcgtgc aagaccgccg tgtccgcgta agactttctc agtccccgaa agctgatcgc
     1981 ggagatatcg cccgctccat ccatcgtcat aattagtgaa ttggatctct tctagttgcg
     2041 caaaagtgca cgcaatcgcg ccaacatgca agcattctgc agcacccacc gctctccgtt
     2101 acaatgctcg cctgtttcga tcccggacat catggcgcat tccgacaacc tcgtagaaat
     2161 aagcggcctg aatttctggt acgacaagcg ccgcccgatc ctgagcggga ttgacatggc
     2221 catcccgcgc gggaaagtgg tttcgatcat gggcatcagc ggttccggca agaccacgct
     2281 gctgcgtctg atcggcggcg cgctgaaacc gcgccaaggc caggtcaagg tagcgggcaa
     2341 agtcatgcac caactcgacc gcgacggcct ctacgagatg cgccgccgta tgggcatgct
     2401 gttccagttt ggcgcgctct tcactgacct gtcggtcttc gataacgtcg cgttccagat
     2461 gcgcgaacac accgatctgc cggaatcgat gattcgcgac atggtgctga tgaagctgca
     2521 cgccgtgggt ctgcgcggcg cgcaccggct gttgccctcg gaactgtccg gcggcatggc
     2581 gcggcgcgtg gcgctggcgc gcgccatcgc gcttgacccg atgctgatca tgtacgacga
     2641 gccgttcgcg ggactcgatc cgatttcgct gggcgtcatt ggcaatctca tccggcggct
     2701 caacgatgcg ctgggggcga cctcgatcgt ggtgacccac gacgtacagg agtcgttgaa
     2761 aatggtcgat tacgtttact acctttccga gggcctgatc gtagccaaag gcaccccgaa
     2821 ggagatgcag gcatcgacgg agccgctggt ccatcagttc gtctatggcg agcccgacgg
     2881 gccggtgccg tttcaatacc cgagcgcgga ttacggcgac gatctgatgc tcaaccgata
     2941 acatgttcaa ccgttcggcg atcgttaacg tcaggtccat cgggcagcac acggtcaacg
     3001 gcctgtggcg gctcggttac gccagccgct ttttcgcgtt gatactgctg cagtccggaa
     3061 ccagtttccg ccgcctgcgc ctgatcatcc gcgagcttta cttcaccggc gtgctgtcgc
     3121 tgatcatcgt tctggtgtcg ggccttttcg tcggaatggt gctcgggctg cagggctatc
     3181 agacgctgca gacttacggc tccgagtctg cgctcggcgt gctggtcgcc ctgtcgctgg
     3241 ttcgagaact gggcccggtg gtctcggcgc tgttgtttgc cagccgcgcc ggttcggcca
     3301 tgacggccga gattggtctg atgaaggcga ccgagcagct atctgcaatg gaaatgatgg
     3361 cggtggaccc gatcgctcgc gttgtggcac cgcgcttctg ggccggggtg atctcgatgc
     3421 cgttgctggc cgccatgttc agtgctatgg gggtcttcgg cggttacttg gttggggtgg
     3481 tgttgatcgg cgttgacgaa ggctcgttct ggtcgcagat gcagaacgcg gttgatttcc
     3541 gcgaggacat cgtcaacggc gtgatcaaga gtttcgtgtt tggtgtcgcc gtcacctgga
     3601 tcgcgctgtt cgagggttac gatgccccgc caactgcaga aggcgtgtcg ggggcgacca
     3661 cgcgtaccgt ggtgacgtc
//
LOCUS       DASQOP010000268         7134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1542438, whole genome shotgun sequence.
ACCESSION   DASQOP010000268 DASQOP010000000
VERSION     DASQOP010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7134
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1542438"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..190
                     /locus_tag="VE030_10475"
                     /pseudo
     CDS             17..190
                     /locus_tag="VE030_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011116969.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            269..1522
                     /locus_tag="VE030_10480"
     CDS             269..1522
                     /locus_tag="VE030_10480"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZF23875.1"
                     /translation="MVTGIIELDRGRLAEYPGSFADYERRKAGQLAAEAVSNAKFDKL
                     LAQEEVWIRRGIEARRTRNEGRVRRLERLRDERAARRNRQGQVTMAVAEGERSGQLVA
                     ELEHVTKRYDGRSVVEDFSCRILRGDKVGLIGPNGIGKSTLIKLILGEIVPDAGKVRR
                     GSRLSVAYYDQLRTRLDEEATVAETIAPGSDYVEIDGARRHVMTYLGDFLFPPERARA
                     PVKALSGGERNRLLLARLFSQPANLMVLDEPTNDLDIDTLELLENLLQDYRGTVLLVS
                     HDRVFLDNVITDYIAAEGAGRWSKNPGGYTEWKRIADEHASAAREQNVHPAPTPARER
                     AGSVRARKLSFAEAKELEGLPARIEALEREHADIGRRLADPALYRDQPQEVQRLNQRY
                     AAIDRELTSFLARWEELEAAQARAG"
     gene            1740..3176
                     /locus_tag="VE030_10485"
     CDS             1740..3176
                     /locus_tag="VE030_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017331588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZF23876.1"
                     /translation="MSFSDLGLAPELLQAIAGQGYTEPTPVQAKAIPPILLGQDVMAA
                     AQTGTGKTASFTLPMLQRLKEFANNSVSPARHQVRALILTPTRELAAQVQESVKTYGK
                     HIALRSAVVYGGINIDPQIKELRAGVEILVATPGRLLDHLQQKTVNLSQVQFLVLDEA
                     DRMLDMGFLPDIKRILSVLPAQRQSLMFSATYSDDIRGLAKQFLKNPVNVEVARRNST
                     VETVTHLVYHVDANAKRDLLTHLIRSRDMRQVLVFCNTKIGANRLAYKLAKDGVAAAA
                     IHSDRTQGERTQALAEFKEGRTRVLVATDVAARGLDIEQLPFVINFDLPGSPEDYVHR
                     IGRTARAGLSGEAIALMADDEREKLDAIEKLLKFKLPVAEVEDFKRSPALRGEARHRE
                     SRRPEGRREHGRRDENRHREDRRPDSGRNPAARSAPSADPLFSKPYNPPASTLTDVAN
                     AAPPPQGTKKQVAALFLPPVAEKQDISS"
     gene            3221..4048
                     /locus_tag="VE030_10490"
     CDS             3221..4048
                     /locus_tag="VE030_10490"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZF23877.1"
                     /translation="MSERLQQVLSGPEEAIDLAEAALLIATHAYPDLDVAHHLARIEE
                     LAKTLELRIQPDSGHSQRILALNQFLFAELGFAANEGDYYDPRNSCLNEVLERRVGIP
                     ITLSLLYMEVGGRVGLPLQGVSFPGHFLVKCALPEGAVVLDPYTGGISLGVADLQKRL
                     RKVHGGEVSRAIVAGMLGAAGKKEIILRLLRNLKAVYLRNQQFDRALPILDWIIATVP
                     DQSSELRDRGMVYQELECSRAALGDLERYLELSPGCDDRDEICSRIVELQQHAARLN"
     gene            4230..5117
                     /locus_tag="VE030_10495"
     CDS             4230..5117
                     /locus_tag="VE030_10495"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZF23878.1"
                     /translation="MFRHPSPYLLLTLASLFWAGNWVVGRAMRNDIPPVAMGFWRWVV
                     ALLILLPFAAPELKRNWRVVRANWFTLALLGGLGAMLFNTMIYVGLQYTAATNGVLFN
                     SVTPILIILMSWLAFRERLGAWQTIGVVLSLVGVAVIVARGEPQILAALRFNRGDLWL
                     ISAMLLWALYTIVLRRRPPGLSAIGFLAAMLLLGLPLLLPFYLWELFTRGGFALSVPA
                     VAALAYYATLPSIVAYLFWNRGVAQIGPNKAGLFVHLMPVFGALLSVIFLGERLYPYH
                     FVGAAMIFGGIWLTTRRSA"
     gene            complement(5278..5922)
                     /locus_tag="VE030_10500"
     CDS             complement(5278..5922)
                     /locus_tag="VE030_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23879.1"
                     /translation="MMPTFHAPLKAIVLAALIAAGVPVRAQNDSHWYGGYSSGFWGLG
                     DSVGFRGSLPWVFGLPGSSPEGLSASRYLSGYRMSDSFAIEGAQTSFGLSGSACGGDP
                     LTGDSFRSCYGSAWRVSGVATLPFRSTGLALYGRMGMHYWQSGSQDEGARHRALDDLG
                     TVYGVGLSYEFSKAVTFRAESERYTDFSGNNGNGPGSGVGLDASVHSIGLSIKF"
     gene            6290..>7134
                     /locus_tag="VE030_10505"
     CDS             6290..>7134
                     /locus_tag="VE030_10505"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZF23880.1"
                     /translation="MTERSQQAEMFLNRVAKNYRHLRKWARREGIESFRVYDRDIPEL
                     PFALDVYGQHAQLQEYSAPVTDVRAQREWRSAMHAAAAQALELPLNQVVLKQRHGQRP
                     DIQYRKLGPRGEDFIVGEGEHRFIVNLTDYLDTGLFLDHRQTRARVQSLAAARRFLNL
                     FCYTGSFSVYAAAGGAKSSTSVDLSNTYLDWARRNFELNGMDAGRHRLVRADARRFVR
                     DEAAAGQRYDLVVLDPPSFSNSKRMQGVLDLQRDHVALIRDCAEILAPAGELLFSTNL
                     RSFRL"
BASE COUNT         1328 a         2325 c         2205 g         1276 t
ORIGIN      
        1 ccgcgacggc tggaacctgc aaagccgggt ggaaacggtg ttgtcgcggc tggggttatc
       61 ggcggatgcc cgcgtcgaag cgctttccgg cggcacccgc aaacgcgttg cgctggcccg
      121 cgcgctggtc gccgatccgg agttgctggt gctcgacgaa cctaccaacc atctcgacct
      181 ggactcgata gcgtggctgg aagagacgct gattgcgttc ccgggtgcgg tgctcgtggt
      241 gacccacgat cgcgcgttcc tggaccgggt ggtcaccggt atcatcgaac tcgaccgcgg
      301 ccggctcgcc gagtatcccg gaagtttcgc cgattacgag cggcgcaagg ccgggcaact
      361 ggcggccgag gcggtgagca atgcgaaatt cgacaagctg ctggcgcagg aagaagtgtg
      421 gatccgcaga ggcatcgagg cgcgccgcac ccgcaacgag ggccgggtgc gccgtctcga
      481 acggctgcgc gatgagcgcg cggcacgccg caaccgccag ggccaggtga cgatggcggt
      541 cgccgagggc gagcgctccg ggcaactggt ggccgaactc gagcatgtca ccaagcgtta
      601 cgacgggcgt tccgtggtcg aggatttctc ctgccgcatc ctgcgcggcg acaaggttgg
      661 cctgatcgga ccgaacggca tcggcaagtc cacgctgatc aaactgatcc tgggcgagat
      721 cgtgccggac gccggcaaag tccgccgcgg cagccgcttg agcgtcgcct actacgacca
      781 gcttcgcacc cggctcgacg aagaggccac cgtggcggag accatcgcac ccgggtccga
      841 ctatgtcgag atcgacggcg cgcgccgcca cgtgatgacc tacctcggcg atttcctgtt
      901 tccaccggag cgtgcccgcg ccccggtgaa ggcgctctcc ggcggcgagc gcaaccgcct
      961 gctgctggcg cgcctgttca gccagccggc gaacctgatg gtgctggacg agccgaccaa
     1021 cgatctcgac atcgatacgc tggaactgct ggagaacctg ttgcaggact atcgcggtac
     1081 cgtgttgctg gttagccacg accgggtgtt cctggacaac gtcatcaccg actacattgc
     1141 cgccgaggga gcggggcgct ggagcaagaa tcccggcggc tacacggaat ggaagcgcat
     1201 tgccgatgag cacgcctcgg ccgcacgcga acagaacgtt catccggctc cgacgccagc
     1261 gcgtgaacgt gccggttccg tgcgtgcgcg caagctcagc ttcgccgaag cgaaggaact
     1321 cgagggcctg ccggcgcgca tcgaggcgct ggagcgggaa cacgcggaca tcggcaggcg
     1381 gcttgccgac ccggcgctct accgggacca gccgcaggaa gtgcagcgtc tgaaccaacg
     1441 ctacgcggcg atcgatcgcg aactcacttc cttcctggcg cgctgggagg aactggaagc
     1501 ggcacaggct cgcgccggtt gacgccgcag ccggcacgcc attcgatctt tacggcattt
     1561 acgtcttctt gtttcacgac attaacgata ttcacggcct tcttggaacc aaaaaagcct
     1621 ttcggaatca cagcttatcc tgtaccatgc gcgctcaatc cgggtccgga ccgagccgcg
     1681 ggccgccgtt tccggcgtca atcgcatcgt cgaatcgaac gatgcaggag ttcaagttaa
     1741 tgtcgttttc tgatctgggg ctcgccccgg agttgttgca ggccatcgct ggtcagggtt
     1801 acaccgagcc tacgccggtc caggcaaagg cgattccccc catcctcctc ggccaggacg
     1861 tcatggccgc cgcccaaacc ggcaccggca agaccgcgag tttcacgctc ccgatgttgc
     1921 agcggctcaa ggagttcgcc aacaacagcg tctcgccggc gcgtcaccag gtgcgcgcgc
     1981 tgattctcac gccgactcgc gaactggccg cacaggtgca ggaaagcgtc aagacctacg
     2041 gcaagcacat cgcattgcgt tccgcggtgg tctacggtgg aatcaacatc gatccgcaga
     2101 tcaaggaact gcgcgccggg gtggaaatcc tggtcgccac tcccggacgc ctgctagacc
     2161 atctgcagca gaagaccgtc aatctttcgc aggtgcagtt cctggtgctg gacgaagccg
     2221 accgcatgct cgacatgggc ttcctgcctg acatcaagcg catcctgtcg gtgctgccgg
     2281 cgcagcgcca gagtctcatg ttttccgcca cgtattccga cgacattcgc gggctggcca
     2341 aacagttcct caagaacccg gtgaacgtcg aggtcgcccg ccgcaattcg acggtcgaga
     2401 ccgtcactca cctcgtgtac cacgtcgatg ccaacgccaa gcgcgatctg ctcacccacc
     2461 tgatccgctc gcgcgacatg cgccaggtgc tggtgttctg caacaccaag atcggcgcca
     2521 accggcttgc ctacaagcta gccaaggacg gcgttgccgc cgccgcgatc cacagcgacc
     2581 gcacccaggg cgaacgcacc caggcactgg ccgagttcaa ggaaggccgc acgcgtgttc
     2641 tggtcgccac cgatgttgcc gcccgcgggc tggacatcga acaactgccg ttcgtgatta
     2701 acttcgacct gcccggttcg cccgaagatt acgtgcaccg tatcggccgc accgcccgcg
     2761 ccggcctgag cggcgaggcc attgcgctga tggccgacga cgaacgcgag aagctggacg
     2821 cgatcgaaaa gctgctcaag ttcaagctgc cggtcgccga agttgaagac ttcaagcgct
     2881 cgccggcgct gcgcggcgaa gcgcgccatc gggaaagccg ccgtccggaa ggccggcgcg
     2941 aacacggccg ccgcgacgaa aaccgccatc gcgaggatcg ccgtcccgat tccggccgca
     3001 atccggcggc gcgcagcgcg ccgtccgcgg acccgctgtt ctccaaaccg tacaacccgc
     3061 ctgcatcgac actgaccgac gtcgcgaacg ccgcgccgcc gccgcaggga acgaagaagc
     3121 aagttgccgc tcttttcctc ccgccggtcg cggagaagca ggacatatcg agttgagtgc
     3181 acgctatccg tccccaacgg aaccccggcc cgcaggcttg atgagcgaac gcctgcaaca
     3241 agtgctatcc ggcccggaag aagcaatcga tctcgccgaa gccgcgctgc tgattgctac
     3301 ccacgcctat cccgatctcg acgtcgcgca ccatctggcg cgcatcgagg aattggccaa
     3361 aactctggag ttgcgcatcc agccggattc gggccactcg caacgcatcc tggcgctcaa
     3421 tcaattcctg ttcgcggaac tcggtttcgc cgccaacgag ggggactact acgacccgcg
     3481 caacagttgc ctcaacgagg tgctggaacg ccgcgtcggc attccgatta cgctcagcct
     3541 gctgtacatg gaggtcggcg gccgcgtcgg cctgccgctg cagggcgtga gtttcccggg
     3601 gcacttcctg gtgaagtgcg cgttgcccga aggcgccgtg gtgctcgacc cgtacactgg
     3661 aggcatttcg ctcggagtcg cggatctgca aaagcgtttg cgcaaagtgc atggcggcga
     3721 ggtttcacgt gccatcgttg ccggtatgct cggcgcggcc ggcaagaagg aaatcatcct
     3781 gcgccttttg cgcaacctca aggccgtcta cctgcgcaac cagcagttcg atcgcgcgct
     3841 gccgatcctg gactggatca tcgctacagt gcccgatcag tcctccgagc tgcgcgaccg
     3901 gggaatggtc taccaggaac tggagtgctc ccgcgccgca ctcggcgatt tggagcgcta
     3961 tctggaactc tcccccggct gcgacgatcg cgacgaaatc tgcagccgta tcgtcgagct
     4021 gcaacagcat gcggcgcgat tgaactagga attccgtgac ggcgtgacga tcgtgacgac
     4081 gtgacgaggt aaaacctacg acgtaagtcc tgatcaaatc aaacgcggca agaagaaaaa
     4141 gaccgatgcc agccaaaacg tcgccgcagg aaagaattgc tccacccgtc acgaccgtca
     4201 cgccgtcacg ttcgtcacgg gttcttgaat tgtttcgtca cccatccccc tacctgctac
     4261 tgactctggc gtcgctcttc tgggccggca actgggtggt cgggcgggcg atgcgcaacg
     4321 acatcccgcc ggtggcgatg ggcttctggc gctgggtcgt tgcgttgctg atcctcctgc
     4381 cgtttgccgc tccggaactc aagcgcaact ggcgcgtcgt gcgcgccaac tggttcaccc
     4441 tcgcgctgct gggcggtctg ggggcgatgc tgttcaacac catgatctat gtcgggctgc
     4501 agtacaccgc agcgaccaac ggcgtattgt tcaattcggt cacgcccatc ctcatcatcc
     4561 tgatgtcgtg gctggcgttt cgcgagcggc tcggtgcctg gcagacgata ggcgtggtgc
     4621 tatcgctcgt gggcgtcgcg gtgatcgttg cgcgcggtga gccgcagata ctcgcggcgc
     4681 tgagattcaa ccgcggcgac ctgtggctga tatcggcgat gctcctgtgg gcgctctaca
     4741 ccatcgtgct gcgccggcgg ccgcccgggc tgtccgcgat cggattcctg gcggccatgc
     4801 tgttgctcgg cttgccgttg ctgctgccgt tttacctgtg ggaactgttt acgcgcggag
     4861 gttttgcgct ttccgtcccc gccgtcgcgg cgcttgctta ctacgccacc ttgccttcca
     4921 ttgtggctta cctgttctgg aatcgtggcg tggcgcagat cggtccgaac aaagcggggc
     4981 tgttcgtcca cctgatgccg gtgttcggcg cgctcctgtc agtgatcttc ctcggtgagc
     5041 ggttgtaccc ctaccacttc gtcggcgcgg caatgatatt cggggggatt tggctgacta
     5101 cgcgccgaag cgcgtagcgc agggacgagg aacgaggggc gagggacgag cgaaatcagg
     5161 gatgacggcg gaaggcggaa ggatgaagta agttcaaagg cttctgactc tccttcatcc
     5221 ctcatccttc atccttggat gctcctccct cgtccctcca tcaaattctg agcgtgctca
     5281 gaatttgatg gacagtccga tcgaatgaac gctggcgtcc agtcccacgc cggagccggg
     5341 accattgccg ttgttgcccg agaagtcggt gtaacgctcc gattcggcgc gaaaagtcac
     5401 ggccttggaa aactcgtagc tcaggccgac tccatatacc gtgccgaggt cgtccagggc
     5461 gcgatggcgg gcgccttcgt cctgcgagcc actctgccaa taatgcatgc ccatcctgcc
     5521 gtacagcgcc aagccagttg atcggaaggg aagagtagcc acacccgata cgcgccacgc
     5581 tgagccgtag caggaccgga aggaatcacc ggtcagagga tcgccgccac aggccgatcc
     5641 gctcaggccg aagctggtct gtgccccctc gatggcaaac gagtcggaca tgcgatagcc
     5701 actgagatac ctcgatgccg acaggccctc cgggctgctg cccggcaacc caaacaccca
     5761 gggcagcgat ccccggaatc cgaccgaatc acccaggccc cagaatccgc tgctgtaacc
     5821 accgtaccag tggctatcgt tctgcgcgcg aaccggcaca ccggccgcga tcagcgcggc
     5881 cagaacgatt gccttgagcg gggcatggaa ggttggcatc atgttttcaa gaagttcctg
     5941 acgcaattct agacaaattt cccagacttt tctgccacgt tttttgctcc cgcagcccct
     6001 cgtaaccggt ttccgaatcc atcggccatg tcacagaccg cgacccaggc gccgctgtcc
     6061 ctcaatcgtc tcgccatgaa ttacaagcac ttaggaaagt ggtgcccgga ctcgttagca
     6121 tcggagccgg ccggaatcgg ctctccggcg ggcgcagctt ggggcggagt cgagtcatgc
     6181 cgagcgtaga cgttgtcatg tgccgagtct tgcctcgcgt tcccgcaaac gagcgtaccg
     6241 ctaacgggtc acgctcgggc gggcagggcg cacacccact tgcatcccga tgaccgaacg
     6301 aagccaacag gccgaaatgt tcctcaaccg tgtagccaag aactaccggc atttgcgcaa
     6361 gtgggcgcga cgagaaggga tcgagtcctt ccgggtgtac gaccgcgaca ttccggaact
     6421 gccgttcgcc ctggacgtgt acggccagca cgcccaattg caggagtaca gtgccccagt
     6481 tacagacgtg cgggcgcaac gcgaatggcg gtcggcaatg catgctgccg cggcgcaggc
     6541 gctcgaattg ccgctaaacc aggtcgtgct gaaacaacgc catggccagc gtccggatat
     6601 ccagtaccgc aagctggggc cgagagggga ggatttcatc gttggcgagg gtgaacatcg
     6661 ctttatcgtc aacctcaccg actatctgga taccgggctg ttcctcgacc accgccagac
     6721 ccgggcacgg gtgcaaagcc tcgccgcggc caggcgcttt ctcaatctgt tctgctatac
     6781 gggaagtttc tcggtctatg cggcagccgg cggcgccaaa tccagcacca gcgtcgacct
     6841 gtcgaacacc tatctggact gggccaggcg caatttcgaa ctcaacggca tggacgccgg
     6901 ccgccatcgg ttggttcggg cggacgcgcg gcggtttgtc cgggacgagg ccgccgctgg
     6961 acaacgctac gatctggtcg tgctcgatcc acccagtttc tcgaactcga aacgcatgca
     7021 gggcgtgctg gacctgcaac gtgaccacgt ggcgttgatc cgggactgtg cagaaattct
     7081 cgcgccggcc ggcgagctgc tgttctcgac caacttgcgc agcttccgcc tcga
//
LOCUS       DASQOP010000269         2740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1744330, whole genome shotgun sequence.
ACCESSION   DASQOP010000269 DASQOP010000000
VERSION     DASQOP010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1744330"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VE030_10510"
     CDS             complement(<1..479)
                     /locus_tag="VE030_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HZF23881.1"
                     /translation="MNLLESLKKLSIIVADTGDIDSIARVKPQDATTNPSLLLQAAQN
                     PAYRPLVDDALAFGMKARGDRAAKAGALMEKLSVNFGREILKHVKGRVSTEVDAGLSF
                     DIEGTLAKARRLIELYQQAGMPRERVLIKVASTWEGIKAAEKLEREGIHCNLTLLFS"
     gene            complement(493..>2740)
                     /locus_tag="VE030_10515"
     CDS             complement(493..>2740)
                     /locus_tag="VE030_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HZF23882.1"
                     /translation="ESIKPHHVKPRLLGHWGTCPGLNLIYAHLNRLIRKFDLNMIYVT
                     GPGHGGPALVANSWLEGSYSEFYPQIPQDQEGMKRLFRQFSFPGGIPSHVAAETPGSI
                     HEGGELGYSLSHAFGAAFDNPDLIVACVIGDGEAETGPLAASWHSNKFLNPAHDGAVL
                     PILHLNGYKIANPAFLARIPREELESLLLGYGYQPHFVEGTDPTQVHQKTAATFEAAV
                     MDIRAIQDTARKSGVAKRPRWPVVVLRTPKGWTGPKEVDGKKTEGSWRSHQVPISDMT
                     KEHIAMLDKWMRSYRPQELFDDSGHPRFELKALAPRGTRRMGANPHANGGALLKDLRM
                     PDFREYTVRVLKPGQTTAEATRVMGALLRDVMKLNASERNFRLFGPDETASNRLGAVF
                     EATAKTWLAQTLPEDDLLAPEGRVMEILSKHTCQGWLEGYLLTGRHGLFSCYEAFIHI
                     VDSMFNQHAKWLKVAGREVPWRRPIASLNYLLTSHVWRQDHNGFSHQDPGFVDHVVNK
                     KADVIRVYLPPDANTLLYVSDRCLRSRNIINVIVAGKQPALQYLDMDSAIRHCAAGIG
                     IWQWAGNDQGGEPDVVMACAGDVPTLETLAAVQLLRRLAPEIKVRVVNVVDLMTLQPS
                     GEHPHGLDDRSFDAIFTADKPIIFAYHGYPWLIHRLTYRRNNHGNLRVRGYKEEGTTT
                     TPFDMVVMNDLDRYHLLMDAVERLPKIAHRAAELKQAMHDKLAEHKRYIAEHGDDMPE
                     IRNWKWET"
BASE COUNT          427 a          879 c          878 g          556 t
ORIGIN      
        1 aagctgaaca gcagggtcag gttgcagtgg atgccttcgc gctccagctt ttccgccgcc
       61 ttgatgccct cccaggtact ggccaccttg atcagcacgc gctcgcgcgg catgccggcc
      121 tgctggtaca gctcgatcag ccgccgggcc ttcgccaatg ttccttcgat gtcgaaggac
      181 aggccggcat ccacttcggt ggatacccgg ccctttacgt gcttgaggat ctcgcggccg
      241 aagttgaccg agagcttttc catcagcgct ccggcttttg cggcgcggtc gcccctggcc
      301 ttcatgccga aagcgagcgc gtcgtccacc agcggccggt aagcggggtt ctgtgccgcc
      361 tgcaaaagca gcgacggatt ggtggtggcg tcctgcggct tcacccgcgc gatggagtcg
      421 atgtcgccgg tgtcggccac gatgatcgaa agtttcttca gcgattcgag caggttcatg
      481 gttgcttccc tttcaggttt cccatttcca gttgcggatc tcgggcatgt cgtcgccgtg
      541 ttcggcgatg tagcgcttgt gctcggccag cttgtcgtgc atcgcctgct tcagttcagc
      601 ggcacggtgg gcgatcttcg gcagccgttc caccgcgtcc atcagcagat ggtaacggtc
      661 gaggtcgttc attaccacca tgtcgaaggg cgtggtcgtc gtcccttcct ccttgtagcc
      721 gcgcacgcgg aggttgccgt ggttgttgcg ccggtaggtg agccggtgga tcagccatgg
      781 gtagccatgg taggcaaaaa tgatcggctt gtcggcagtg aagatggcgt cgaaagagcg
      841 gtcgtcgagc ccgtgcggat gctcgccgga cggttgcagc gtcatcaggt ccaccacgtt
      901 gaccacccgc accttgatct ccggcgcgag gcgtcgcagg agctgcaccg ccgccagcgt
      961 ctccagcgtc ggcacgtccc ccgcgcaggc catcaccaca tccggctccc caccctggtc
     1021 gttgcccgcc cactgccaga tgccgattcc cgcggcgcaa tgcctgatcg cggaatccat
     1081 gtcgagatac tgcagcgccg gctgcttgcc ggccacgatc acgttgataa tgttgcggct
     1141 gcgcaagcac cggtcgctga cgtagagcag cgtgttggcg tccggcggca ggtatacgcg
     1201 gatcacgtcg gccttcttgt tgacgacgtg gtctacgaag ccggggtcct ggtgcgagaa
     1261 gccgttgtgg tcctggcgcc agacgtgcga cgtcagcaga tagttgagcg aagcgatcgg
     1321 ccggcgccac ggcacttccc tgcccgccac cttcagccac ttggcgtgtt gattgaacat
     1381 cgagtccacg atgtggatga atgcttcgta gcaggagaac agtccgtggc gaccggtcag
     1441 cagatagcct tcgagccagc cctggcaggt atgcttggag aggatctcca tcacgcggcc
     1501 ctccggcgcg agcagatcat cctccggaag cgtctgcgcc agccaggtct tcgcggtcgc
     1561 ctcgaagacc gcaccgagcc ggttcgacgc ggtttcgtcc gggccgaaca gccggaaatt
     1621 gcgctcgctg gcattgagtt tcatcacgtc gcgcagcagg gctcccatca cccgcgtggc
     1681 ttcggcggtg gtctgccccg gtttgagcac gcgcaccgtg tactcgcgga agtcgggcat
     1741 gcgcaagtcc ttgagcaacg cgccgccgtt ggcgtgcgga ttggcgccca tgcgccgagt
     1801 tccgcgcggc gctaaggcct tgagttcgaa gcgcggatgg ccgctgtcgt cgaacagctc
     1861 ctgcgggcgg tagctcctca tccacttgtc gagcatcgcg atgtgctctt tggtcatatc
     1921 gctgatcggc acctgatgcg agcgccacga gccttcggtt ttcttgccgt ccacttcctt
     1981 cggtccggtc cagcccttgg gcgttcgcag caccaccacc ggccagcgcg ggcgcttcgc
     2041 cacgccgctc ttgcgcgccg tgtcctggat tgcgcggata tccatcaccg cggcctcgaa
     2101 tgtcgccgcc gtcttctggt gtacctgcgt cgggtcggtg ccctctacga agtgcggctg
     2161 gtagccatag ccgaggagca ggctctccag ttcctcgcgt ggaatgcgcg ccagaaacgc
     2221 gggattggcg atcttgtatc cgttcaggtg caggatcggc agcaccgcgc catcgtgcgc
     2281 cgggttgagg aatttgttgg agtgccagct ggccgcaagc ggcccggttt ccgcctcgcc
     2341 gtcgccgatc acgcaggcca cgatcaggtc cgggttgtcg aacgccgccc cgaacgcatg
     2401 cgaaagcgag tagccgagtt cgccgccctc gtgtatcgaa cccggcgttt ccgccgcgac
     2461 atggctggga attccgcccg gaaaggagaa ctgccggaac agccgtttca tcccttcctg
     2521 atcctgcggg atctgcggat aaaactcgct gtagctgccc tccagccagg aattcgccac
     2581 cagcgccggg ccgccgtgcc cgggtccggt gacatagatc atgttgaggt cgaatttgcg
     2641 gatcagccgg ttaagatgcg cgtagatcag gttgagcccg gggcaggtac cccagtggcc
     2701 cagcaggcgc ggcttgacgt gatgaggctt gatcgactcg
//
LOCUS       DASQOP010000270         6111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_535808, whole genome shotgun sequence.
ACCESSION   DASQOP010000270 DASQOP010000000
VERSION     DASQOP010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6111
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535808"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="VE030_10520"
     CDS             <1..662
                     /locus_tag="VE030_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HZF23883.1"
                     /translation="TAVSHQLDNNGDSRMIELYFAPTANGLRAAVALEETGLPYRTHK
                     VDLAKGEQRSPEFLKLNPAGLIPVIVDPDGPGGKPLTLSQSGAIILYAAEKSGKFLPK
                     DAARRARAMQWLMQAASDIAGTSGTIFRMETSIPEKIASNADYFKQRLLGFFTVCDAQ
                     LAAREYLADEFSVADIMLYPNFAARKALIDQAGGFANLQRWGAAIGSRPGVQRGMKIT
                     G"
     gene            complement(664..1086)
                     /locus_tag="VE030_10525"
     CDS             complement(664..1086)
                     /locus_tag="VE030_10525"
                     /inference="COORDINATES: protein motif:HMM:NF042552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6647 family protein"
                     /protein_id="HZF23884.1"
                     /translation="MDQSSLSSLVVELFTTIQLLAGYPAPQSGPEIHIVPHAVIEEKI
                     CRSQCRRIKAFYHPDWGVYVDESLDLAGDAFDRSILLHELMHHVQHVAGAYEMLPSDC
                     ERRNAEEQEAYKIQNKYLAAIGDPRRIPAGAWMGPCRN"
     gene            complement(1220..1666)
                     /locus_tag="VE030_10530"
     CDS             complement(1220..1666)
                     /locus_tag="VE030_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23885.1"
                     /translation="MDPVSLSALVAELFAAIQLLSGYAPPTVPPEIHRVPQTVIQEQF
                     CRRPCPVRAAYDAARGVFIDENLDVENNTFDRSILLHELIHHAQAVSGRFDTLSSDCM
                     RRNWAEKEAYFIQNRYLMEMNSASRVSMRGWAARCDDAEVPTPTRQ"
     gene            complement(1917..2354)
                     /locus_tag="VE030_10535"
     CDS             complement(1917..2354)
                     /locus_tag="VE030_10535"
                     /inference="COORDINATES: protein motif:HMM:NF042552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6647 family protein"
                     /protein_id="HZF23886.1"
                     /translation="MDQSSLTSLMVELFTAIRLVSGYPSPDVLPQIQRVTVVEMQQKI
                     CGGRSCGVKAFYHPEWGVYVDEALDIQNSAFDRSILLHELVHHLQKTTGKFDAVANFC
                     YRRNAQELEAYQIQNRYLADQGAPRRALVMGWSGTCKDEDAER"
     gene            complement(2461..2904)
                     /locus_tag="VE030_10540"
     CDS             complement(2461..2904)
                     /locus_tag="VE030_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23887.1"
                     /translation="MDHALASLVTDLLTTIHLLSGYPAVSVQPEIHRLPQAAIAERVC
                     HKPCGVRAYYRPDWGVLLDESLNVESDAFDRSILLHELVHYVQAARGRFDTMPSACDR
                     WNAAEREAYGLQNQFLADVHSAKHVAMSGWAARCDDKDSTAGARH"
     gene            complement(3230..4072)
                     /gene="rlmJ"
                     /locus_tag="VE030_10545"
     CDS             complement(3230..4072)
                     /gene="rlmJ"
                     /locus_tag="VE030_10545"
                     /inference="COORDINATES: protein motif:HMM:NF016278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HZF23888.1"
                     /translation="MLAYRHLFHAGNFADVFKHSLLTQLVLALEKKDKPFFVLDTHAG
                     TGLYDLTHAWAQKNAEYKDGIALLGERKDVPNALVPYLEAVRAENEGGKLRFYPGSPR
                     IVRRLLRPNDRMVLIELNRKDCEELEALFRGDRQVMVRLMDGYQALRAFLPPKERRGL
                     VFIDSSFDRAQEFKRLTEGLVEAHRRFATGVYGLWYPLMEPTAMRAFERGVMATGIPK
                     ILQLELSVMPDDWGGNMRGCGLLVVNPPFGFEEIARSILDWLWPVLSRERDGGHTVRW
                     LAGE"
     gene            4241..4624
                     /locus_tag="VE030_10550"
     CDS             4241..4624
                     /locus_tag="VE030_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23889.1"
                     /translation="MAADNAPELKMDPANLYREEVFTDRKVGTIRVMTPVKRDASTDA
                     GRKVIYVGETQLMTPVGALPLAFEIEADSLGNAAAKFAEVAQVAVERAVRELQEMRRQ
                     ASSSIVIPEGIPPGLGGPGGKIKLP"
     gene            complement(4733..5737)
                     /locus_tag="VE030_10555"
     CDS             complement(4733..5737)
                     /locus_tag="VE030_10555"
                     /EC_number="7.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006119 - oxidative phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HZF23890.1"
                     /translation="MTAMQLDLPLIFMVLMGFAILAYVILDGFDLGVGILLPLATREE
                     QDMMVASIGPFWDANETWLVLGVGILLVAFPHAHGVILSSLYLEVALMLIALILRGVA
                     FEFRVKASGWHQDLWNRLFFVGSLGAAIAQGMMLAGVITGFGEGALFVAFRLLVGVGL
                     AGGYALLGSTWLVIKTENDLRARALRWGIFAVILTAAGVAAISLATPFMHAGIMEKWF
                     SWPRFLWLAPLPLLTAAACAGILVSLIQLERGAAQREWLPFVLSVWVFVFAFAGLAYS
                     LFPYLVVDRITIWQAAAARESLMFVFVGACVVLPVIVAYTVFSYRVFWGKARSLTYGE
                     "
     gene            complement(5734..>6111)
                     /locus_tag="VE030_10560"
     CDS             complement(5734..>6111)
                     /locus_tag="VE030_10560"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HZF23891.1"
                     /translation="GVLMLALSWSGAWYLLRRREIPRRLLPGFVAMAFSGWVAVLAGW
                     LTTEIGRQPYLVYGVITTAETASDVPAPYIALTLVSYLVVYTLLLISYMVVLTQLALK
                     EADGNGHRQPSRAALQPAGAQGS"
BASE COUNT         1253 a         1945 c         1802 g         1111 t
ORIGIN      
        1 aaactgcagt gtcgcaccaa ctcgacaaca atggagatag ccgcatgatc gaactttact
       61 tcgcgccgac agccaatggg ctgcgcgcgg cggtagcact ggaggagacc ggcctgccct
      121 accggaccca caaggtggac cttgccaagg gcgaacagcg tagccccgaa ttcctgaagc
      181 tcaatcctgc cgggctgatc ccggtgatcg tcgacccgga cggccccggc ggcaaaccgc
      241 tcacgctctc tcagtcagga gcgatcatcc tttatgccgc ggagaagtcc ggcaagttcc
      301 tgccgaagga tgccgcacgg cgcgcgcgcg ccatgcagtg gctgatgcag gcggcgagcg
      361 acatcgccgg caccagcggc accatctttc gcatggaaac gagcatcccg gaaaagattg
      421 cttcgaacgc cgactatttc aagcaacggc tgctcggctt tttcacggta tgtgatgcgc
      481 aattggctgc gcgcgagtat cttgcggacg agttctccgt cgccgacatc atgctgtatc
      541 cgaactttgc ggcgcgcaaa gcgctgatcg accaggccgg cggatttgcc aatctgcaac
      601 gttggggggc ggcaatcggc tcgcgacccg gggtccagag gggaatgaag ataacgggct
      661 gattcaattc ctgcacgggc ccatccatgc gccggccgga atccgtcgcg gatcgccgat
      721 ggccgcgagg tacttgttct ggatcttgta ggcttcttgc tcttcggcgt tgcggcgttc
      781 gcagtccgaa ggcagcatct cgtacgcgcc cgcgacgtgc tggacgtggt gcatcagttc
      841 atgcagaaga atcgatcgat cgaaagcgtc acccgccaga tcgagtgatt cgtccacgta
      901 cacgccccag tcagggtggt agaaggcctt gatgcgcctg cactggctcc ggcagatttt
      961 ttcctcgatc accgcatgcg gaacgatgtg gatttcggga ccggactgcg gtgccggata
     1021 gcctgcaagt aactgaatcg tagtaaaaag ttcgaccaca agggaggaaa gggaagactg
     1081 gtccattgga ctcctttggc ccgccccctc ctcccaatgc gcaggaccgt tgtgttacct
     1141 catcgtgtct ccttagttat ttcttaacca tgcaagattc gagccttacc gggcggtccg
     1201 gcggcctgga aaacctggtt cactgcctcg tgggtgtcgg cacttccgcg tcatcgcagc
     1261 gcgcggccca gcccctcatc gatacgcggc tcgcgctgtt catttccatc aagtaacgat
     1321 tctggatgaa ataggcttcc ttttccgccc agttgcgtcg catgcaatcg ctcgacagcg
     1381 tatcgaatcg gccgctgacc gcctgcgcgt ggtggatcag ctcgtggaga aggatggaac
     1441 ggtcgaaggt gttgttctcg acgtcgaggt tttcatcgat gaacacgcct cgcgcggcgt
     1501 cataagcagc ccgcaccgga cagggccggc ggcagaactg ctcctggata acggtctggg
     1561 gcacccggtg aatttcgggg ggcaccgtcg gcggcgcata gccggacagc agttgtatgg
     1621 ctgcgaagag ttcggcaacc agagcagaca aagagaccgg gtccatgggg actctcccac
     1681 ccggcgtccc tccgccgctc gggcatcgaa gttggatcgt tatccgtttc gcatgctaca
     1741 agtacgaccg ccttcaacgg ctcaagtttc gggtaattcg tagaccaaac gcactgaaaa
     1801 tagacgggac agttgatgaa gttgtgtctt aattcgtggc gatgcggacc gcggcaagac
     1861 tttccgccgc agaccgaagt ctttgcgcgc cagtaaatcg ttctcggctc ttgccctcat
     1921 cgttccgcgt cctcgtcctt gcacgttcct gaccagccca ttacgagagc gcggcgcggt
     1981 gcgccctggt cggcaaggta gcgattctgt atctggtagg cctccagttc ctgggcattg
     2041 cgccggtagc agaagttcgc tacggcatcg aacttgccgg tggttttctg caggtggtgc
     2101 accagttcgt gcaggaggat cgagcggtcg aaggcactgt tctggatgtc gagcgcctcg
     2161 tccacgtaga cgccccactc ggggtggtag aaggctttga cgccacagga gcggccgccg
     2221 cagattttct gttgcatttc gactacggtt acacgctgga tttgcgggag gacatccggg
     2281 ctgggatacc cggacacgag cctgatcgcc gtgaacaatt cgaccatcag tgacgtcagt
     2341 gaagactggt ccatggcaac tccttcgcct ggttagtctc cccctgcggg catttaatcg
     2401 gcatcgcggt caccggtcaa gcggggctcc tgcgtcgtgg tccggcctgg tcatcaccgg
     2461 tcaatgacgg gctcccgccg tggaatcctt gtcgtcgcaa cgcgcggccc agccggacat
     2521 ggccacgtgc ttcgcgctgt gtacgtcggc gagaaactgg ttctgcaggc cataggcctc
     2581 gcgctcggcc gcgttccagc gatcgcatgc gctcggcatg gtgtcgaacc gccctcttgc
     2641 ggcttgcacg tagtgaacca gttcgtgcag cagaatcgag cggtcgaaag cgtccgattc
     2701 cacgttcagc gactcgtcga gcagaacgcc ccaatccgga cggtaatagg cgcgcacgcc
     2761 acagggtttg tggcagaccc tttccgcgat cgcggcctgg ggtaaccgat gaatttcagg
     2821 ctggacggaa accgctgggt agcctgacag caggtgaatg gtggtcagca gatccgtcac
     2881 cagcgaagca agagcgtgat ccacagtcgg ccttccgacc cggtgctcct cccacgactc
     2941 gggcaacaaa ttgacggttc ttaaagatct tgcttttagc tccgcaagaa tcgggccacg
     3001 cccaccggcg tggccaaacc ccgttcatcc ccgggaatgc accgttccgc cgcatgttcc
     3061 gcatcatcct gccctaaagc ggggcgggcc tgccccggat tggaatccgc caggtagtcc
     3121 cgttccaggt caatcgccag tcttgcctcg ggattttcct ccgccgtaag cttttgcccc
     3181 ttcacccttc acccttcatc cttccgcctt catccttcat ccttgccctt cactccccgg
     3241 ccagccacct tacggtgtgt ccgccgtccc gctcccgcga cagcaccggc cacagccagt
     3301 caagtatgga ccgcgcgatc tcctcgaatc cgaacggcgg gttcacgacc agtagtccgc
     3361 agccacgcat gtttccaccc cagtcatccg gcatcaccga gagttccagt tgcaaaattt
     3421 tgggtattcc ggttgccatc acgccacgct cgaatgcccg catcgcggtc ggttccatca
     3481 gcggatacca aagcccgtac accccggtcg cgaaccgccg gtgagcctcc accaatcctt
     3541 cagtcaggcg cttgaactcc tgcgcgcggt cgaaggagga atcgatgaac accagtccac
     3601 ggcgttcctt gggcggcagg aaggccctga gtgcctggta gccatccatc aggcgcacca
     3661 tcacctgcct gtcgccgcga aacagtgctt ccagttcttc gcagtccttc ctgttgagtt
     3721 cgatcagcac catgcggtcg ttcggccgaa gcagccggcg cacgatccgc ggggagccgg
     3781 gatagaaacg cagcttgccg ccctcgttct ccgcgcgcac cgcctcgagg tacggcacca
     3841 aagcattggg tacatccttg cgctccccca ggagcgcgat accgtccttg tattccgcgt
     3901 tcttctgcgc ccaggcgtgc gtcagatcgt aaagcccggt tcccgcatgc gtgtcgagca
     3961 cgaagaaggg tttgtccttc ttttccagcg cgagcacgag ctgcgtcagc agcgagtgct
     4021 tgaacacgtc cgcaaaattg ccggcatgaa aaagatgtcg gtacgcgagc attgagcgat
     4081 gacgggtgat gggtgaggga acaagtatga aggatgaagg cagaaggatg aataaaggcg
     4141 gcgcctgatc acgagcagtt tacgcactcg ggcaccattt caacgagccg tgggataatt
     4201 ccggaagtca cttgcgcatt tcgaaccgga gacaaggacc atggccgcag acaacgcccc
     4261 cgaactcaaa atggatcccg cgaaccttta tcgcgaggag gtcttcaccg accgcaaggt
     4321 cggcaccatt cgcgtcatga cgccggtgaa aagggacgcc tccaccgacg ccggccgcaa
     4381 ggttatctac gtcggcgaaa cccagctcat gacgccggtc ggagcgttgc cgctcgcctt
     4441 cgagatcgag gccgactcgc tcggcaatgc cgccgcgaag ttcgccgaag tcgcgcaggt
     4501 cgcagtcgag cgtgcagtca gggaacttca ggaaatgcgc cgccaggcca gttcgtcgat
     4561 cgtcatcccc gaaggcatcc cgcccgggct cggcggaccc ggcggaaaaa tcaaactgcc
     4621 gtaggaaaca aatttagggg cgagggacga ggggcgaggg aacggcaaga accggaaata
     4681 aagattcaag aaacgacgta tcgactcacc cctcatccct cgcccctcgt ccctattctc
     4741 cataggtgag tgaacgggcc ttcccccaga acacccggta cgaaaacacg gtgtaggcca
     4801 cgatgaccgg caacaccacg caggcgccga cgaatacgaa catcagcgac tcgcgtgccg
     4861 ccgccgcctg ccagatggtg atacggtcca ccaccaggta cggaaacagg ctgtaggcca
     4921 ggccggcgaa cgcgaacacg aacacccaca ccgacaatac gaaaggcagc cattcgcgct
     4981 gcgcggctcc gcgctcaagc tggatcagcg ataccaggat gccggcgcat gcggcggcgg
     5041 tgagcaacgg cagaggcgcc agccacagga agcgcggcca ggagaaccat ttttccatga
     5101 tgcctgcgtg catgaacggc gttgccagac tgatcgctgc gacccctgcc gcagtcagta
     5161 tcacggcgaa aattccccag cgcagcgccc gcgcgcgcag gtcgttctcg gttttgatca
     5221 ccagccacgt cgagcctagc agcgcgtatc cgccggccag ccccacgccc accagaagtc
     5281 tgaaggcaac gaaaagagcg ccctccccga agccggtgat gacgccggcg agcatcatcc
     5341 cctgggcgat cgccgccccg agcgagccga cgaagaacag ccggttccat aaatcctgat
     5401 gccagcctga agcctttacc cggaactcga aagccacgcc gcgcaggatg agcgcgatca
     5461 gcatcaaggc aacttccagg tagagcgaag acaggatgac gccatgtgca tgcgggaacg
     5521 ccaccagcag gattcccaca ccgagcacca gccaggtttc gtttgcgtcc cagaagggac
     5581 cgatcgaagc caccatcatg tcctgctcct cgcgcgtggc cagcggcagc aggattccca
     5641 ctccgaggtc gaaaccgtcg aggatgacgt aggcgaggat ggcaaacccc atcagcacca
     5701 tgaagatcaa gggcagatcg agttgcatag cggtcatgat ccctgcgcac ccgccggctg
     5761 gagcgcggcg cgcgacggct gccggtgccc attgccgtcc gcctctttga gtgcgagctg
     5821 ggtgagcacc accatgtatg aaatcaacaa cagcgtatac accaccagat aactgacgag
     5881 cgtgagcgca atgtacggag ccggcacgtc cgaagcggtt tcggcggtgg tgatcacgcc
     5941 gtacacgagg tagggttggc ggccgatctc cgtggtgagc cagcccgcca gcactgcaac
     6001 ccatccggaa aacgccatcg cgacaaaacc cggcaggagc ctgcgcggga tctcgcgccg
     6061 ccgcaacaga taccaggcgc ccgaccatga cagcgccagc atcagcacgc c
//
LOCUS       DASQOP010000271         6100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1543922, whole genome shotgun sequence.
ACCESSION   DASQOP010000271 DASQOP010000000
VERSION     DASQOP010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6100
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543922"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VE030_10565"
     CDS             complement(<1..190)
                     /locus_tag="VE030_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23892.1"
                     /translation="MSMAGQDGAVAEAIAAGGWAVVPDFIESGLISDLRAECRRFVAQ
                     GVLRAAAVGSGARRQTRSD"
     gene            complement(196..1692)
                     /locus_tag="VE030_10570"
     CDS             complement(196..1692)
                     /locus_tag="VE030_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA synthase"
                     /protein_id="HZF23893.1"
                     /translation="MNPNFYAAIEAANRGRLQHDLLETETGRTFTYGDLAGESARYAN
                     VLTGLGARRGDRIAVLVEKSSASLFLYLGCLRAGLVYVPLNTAYQRGELSYFLDDAEP
                     RVIVCRPQAADLIQDLYGSRSQIFTLDEDGGGSLAHAAAESRHDFATVEMQPGDSAVI
                     IYTSGTTGRSKGALVTHRNLASNARVLVDYWQFGANDVLLHALPMFHVHGLFVANHCA
                     LLAGAKLLWHGKFDVKLVIRDLARASVMMGVPTFYNRLLSESTFTRALCNNVRLFVSG
                     SAPLQLETFKDFEARTGKTILERYGMSEAGMISSNPFAGERRGGTVGFPLPGISVRIV
                     DERDRPVENGVIGAIQVKGENVFAGYWRMSDKTREEFTADGYFRTGDVGVFDRDGYLS
                     IVGRAKDLIISGGYNVYPREIELVLDAIPGVAESAVIGVPHPDFGEAVTAVVVAQKDV
                     RLDPAAVIGAVKNTLANYKVPKAVHFVDDLPRSAMGKVQKALLREKFR"
     gene            complement(1692..2957)
                     /locus_tag="VE030_10575"
     CDS             complement(1692..2957)
                     /locus_tag="VE030_10575"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit gamma"
                     /protein_id="HZF23894.1"
                     /translation="MSSDSFSASSPAQRVAALLDPGSFRGIADEARGALTAGTGGVGD
                     TAVAIAATDRHIAGGSFGVVESQALSALLQHSRNDGVPVILCLDSAGARLDEGLPALG
                     AFRQLYRHFLDLRLAGVPTLAVLGRDCFGGASMLAAACGQRVYSPVSRLAMSGPAVIQ
                     ALGGRQELDAEDAEAVTALMGGAARVRLQPADRLCMDTPGDFRQAALEWTEQRDPESQ
                     LDLRDQHNQLGTRLLERGMAPSPQSNAETAPPAALLEVVPPGFQARTIDGVLIGRTTK
                     NSPDTFFGLVSREPVGALALWTLAGACLSAADSRPGEAIMLLLDAPGQAPTLRDERVM
                     LSQYVAHLALVLSHLRLSGHRVTLQVLGEAAGGIYVALAAPATRVVALPGANVQILPP
                     AAMARVIGRHSARGNVDDYLRAGVVDTII"
     gene            complement(2979..4109)
                     /locus_tag="VE030_10580"
     CDS             complement(2979..4109)
                     /locus_tag="VE030_10580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HZF23895.1"
                     /translation="MAAHIDAVELTRQIVRMNTINPPGDEEQCARHLGVLLENAGFKV
                     AYHRLSPKRASLVATIGGSSDKAPLCFTGHIDTVPLGAAKWKMGPFAADTDAGKLYGR
                     GTSDMKSGVAAFVVACVNLSGKLERSPGLELVITAGEETGCQGAFHLTQKSGALGRAG
                     AVVVGEPTSNYPLVGHKGAFWLNARTKGVTAHGSMPEKGVNAVFKAARALAVLEKFRF
                     SNPPHPLMGQATLNVGTIRGGLNINSVPDEAVIGIDIRTVPNVRHSQLKTELERQLGA
                     DVELETLLDLESVYTAPEDPWVQEVFDVMRPILGSRPEPRAATYFTDAAALSIAYRTP
                     PTIVLGPGEAQLAHQTDEYCVVERVAESVAAYEEIARRWCNI"
     gene            complement(4169..4348)
                     /locus_tag="VE030_10585"
     CDS             complement(4169..4348)
                     /locus_tag="VE030_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23896.1"
                     /translation="MRENSPKSSYRRTPGAKPLARLADVLEAFPRLKERVRRLAGTMS
                     GGEHAMLSIRRGSAS"
     gene            4485..6059
                     /locus_tag="VE030_10590"
     CDS             4485..6059
                     /locus_tag="VE030_10590"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZF23897.1"
                     /translation="MKVVFALLRAFVLALAFAASSAAAQEKVLKFIPQSDLRILDPIT
                     TTAYITRNHGYMVYDTLFATNAKFQIQPQMVNKWELSRDKLTYTFTLRDGLKFHDGQP
                     VRSADCIASIERWGKRDSLGQRLAQSTESWTAVNDKIFKLRLKKPFPLTLDALGKLSS
                     NVPFIMPERIAKTDPFQNITEAIGSGPFKFVKEEWVPGSKVVYVKNTDYVPRKEAPSW
                     AAGAKVAKVDRVEWIYIPDSATAAAALSAGEADWWEQLPPDLIPLLRKNKDVRVENID
                     PLGSIGMIRFNFLQPPFNNQKLRQALLYVVNQRDYVLGIAGDEKNGHTCYSFFTCGTP
                     LSSEIGAEPLKGKRDFDKARKLIQESGYKGEKIVIISATDQPIVHSQSLLTADLLRNL
                     GLNVELQAGDWGTLITRRTSKEPVEKGGWSIFHTWWVGPDMISPANMAMVNLGDKSWF
                     GWPADPRLEELRQAWFNTTDPASGKKAADAVQLEAFNFVPYITTAQFILPAAYRSNLS
                     GIIVAPVTFLWNVEKK"
BASE COUNT         1106 a         2043 c         1871 g         1080 t
ORIGIN      
        1 tgtcgctgcg ggtctgccgg cgggccccgc tgccaaccgc cgcggcacgc agcacacctt
       61 gcgcaacgaa ccggcggcac tccgcgcgca gatccgagat cagtccggat tcgatgaaat
      121 ccggaacgac cgcccagcct ccggcggcaa tggcttcggc gacggcaccg tcctgtccgg
      181 ccatcgacac gcaggctagc ggaatttctc ccgcagcagc gccttctgca ccttgcccat
      241 ggcactgcgc ggcaggtcgt cgacgaagtg gaccgctttc gggactttgt aattggccag
      301 cgtgtttttc accgcgccga tgactgcagc gggatccagg cggacgtctt tttgcgccac
      361 aacgaccgcg gtgactgctt cgccgaaatc aggatgcggc acgccgatga ccgccgattc
      421 ggccacgccc ggaatggcgt cgagtaccag ctcgatttcc ctcgggtaga cgttgtaacc
      481 gccgctgatg atcaggtctt tggcgcggcc gacgatggag agataaccat cgcggtcgaa
      541 cacaccgaca tcgccggtgc ggaagtatcc gtcggcggtg aactcctccc gggtcttgtc
      601 cgacatgcgc cagtagcccg caaacacgtt ttcacccttc acctgaatcg cgccgatgac
      661 cccgttttcc accggccggt cgcgctcgtc cacaattcga accgatatcc ccggcagcgg
      721 gaaacctacc gtgccgccac ggcgttcacc ggcgaacggg ttggagctga tcatgccggc
      781 ctcggacatg ccgtagcgct ccagtatggt cttgccggtg cgcgcctcga agtccttgaa
      841 ggtctcgagc tgcagcggcg ccgaaccaga cacgaacagc ctgacgttat tgcacagcgc
      901 gcgcgtgaac gtggattcgc tcagcagccg gttatagaac gtcggcacgc ccatcatcac
      961 gctggcacgc gccaggtcgc gtatgaccag cttcacatcg aactttccgt gccacagcag
     1021 cttcgcgccg gccagcagcg cgcagtgatt ggcgacgaac aagccgtgca cgtgaaacat
     1081 cggcaacgcg tgcagcagca cgtcgttcgc gccgaattgc cagtagtcca ccagtacccg
     1141 ggcgttcgac gcaaggtttc ggtgcgtgac tagcgctcct ttggagcgcc cagtcgtgcc
     1201 cgaagtgtag atgatcaccg cggaatcgcc gggttgcatc tccacggtcg caaaatcgtg
     1261 tctactctcg gccgccgcgt gcgccaggga cccgccgcca tcctcgtcca gggtgaagat
     1321 ctgcgaacgg ctgccgtaca gatcctggat cagatcggcc gcctgtggcc ggcacacgat
     1381 caccctgggc tcggcgtcat cgagaaagta cgacagttcg ccgcgctggt atgcggtatt
     1441 gagcggcaca tataccagtc cggcgcgcag acagccgaga tacaggaaca aagatgcaga
     1501 ggatttctcc accagcaccg caatccggtc gccgcgtctc gcgccgagac cggtcaacac
     1561 gttcgcatag cgcgccgatt cgcccgccag atcgccgtag gtgaatgtcc tgccggtttc
     1621 cgtctccaga agatcgtgct gcaggcggcc gcggttcgcc gcttcgattg cggcgtagaa
     1681 attcgggttc atcagataat ggtgtcgacc acgcctgcgc gcaaatagtc gtccacattg
     1741 ccgcgcgcac tgtggcggcc gattacccgc gccatcgctg ccggcggcag tatctgcacg
     1801 tttgcgcccg gcagcgccac cacacgcgtt gccggtgccg ccagcgcgac atagattccg
     1861 cccgccgcct cccccagaac ctgcagcgtg acgcggtggc cgcttagcct gagatgcgag
     1921 agcacgagcg caagatgggc gacgtactgg gaaagcatga cgcgctcgtc gcgaagagtc
     1981 ggtgcctgtc ccggagcgtc gagcagcagc atgattgctt cgccggggcg tgaatccgcc
     2041 gccgacaggc acgcgcccgc cagcgtccac agtgccagtg ctcccaccgg ctcgcggctt
     2101 accaggccga aaaaagtatc gggcgagttt ttcgtggttc tgccgatcag aacgccgtcg
     2161 atggtgcgtg cctgaaaacc cggcggcacc acttccagca acgccgccgg cggcgccgtc
     2221 tcggcattcg actgcggcga gggcgccatt ccgcgctcga gcaaccgcgt gccgagttgg
     2281 ttgtgctggt cgcgcaagtc gagctgcgat tccggatcgc gctgctccgt ccattccagc
     2341 gccgcctggc gaaaatcgcc cggcgtgtcc atacacaggc gatccgccgg ctgcaggcgc
     2401 acgcgcgccg cgcctcccat cagcgccgtg accgcctcgg cgtcttcggc gtcgagttcc
     2461 tgtcgtccgc ccagcgcctg gatcaccgcc gggcccgaca tcgccaaccg cgagactggt
     2521 gaatacacgc gctgtccgca ggccgccgcc agcatgctgg caccgccaaa gcagtcgcgt
     2581 cccagaactg cgagcgtcgg tactccggca agcctgagat cgagaaagtg gcgataaagc
     2641 tggcggaatg cgccaagcgc aggaaggcct tcgtcgagcc gcgcgccggc cgaatccaga
     2701 cacaggatca ctggcacgcc gtcgttccgg ctgtgctgca acaacgcgga aagcgcctgc
     2761 gactcgacta caccgaaaga gccgccggca atgtggcggt cggtcgccgc gatcgccacc
     2821 gcagtatcgc cgacgccgcc ggtgcctgca gtcaatgcgc cgcgcgcttc atccgcgatt
     2881 ccccggaacg aaccgggatc gagcagcgca gcaacccgct gcgccggact gctggccgag
     2941 aaggaatcgg aagacatcgc tgatcgccct acgccccgct agatgttgca ccagcggcgc
     3001 gcgatctcct cgtatgcggc aaccgattcg gcgacgcgct cgactacgca gtactcgtcg
     3061 gtctggtgcg ccagctgcgc ctcgcccggc ccgagtacaa tggttggcgg cgtgcgatag
     3121 gcaatactga gtgccgctgc atcggtgaaa taggtcgccg cccgcggttc gggccggctg
     3181 ccgagaattg ggcgcattac atcgaacacc tcctgtaccc agggatcttc gggtgcggtg
     3241 taaacacttt ccaggtcgag cagcgtctcg agctccacgt cggctcccag ttggcgttcg
     3301 agctcggtct tgagctggga gtgcctgacg ttgggaacgg tgcggatgtc gatgccgatc
     3361 accgcttcgt ccggaaccga attgatgttc agtccgccgc ggatggtgcc gacgttgagc
     3421 gtggcctggc ccatcagcgg gtgcggcgga ttggagaagc ggaatttctc cagcaccgca
     3481 agcgcccgcg ccgccttgaa caccgcgttg acgcccttct ccggcattga gccgtgcgca
     3541 gtgacccctt tggtgcgggc gttgagccag aacgcgccct tgtggccgac caacggataa
     3601 ttcgacgtcg gctcgcccac caccacagcg cctgcgcgcc cgagcgcgcc gctcttctgc
     3661 gtgagatgaa atgcgccctg gcagccggtt tcctcgccgg cggtgattac cagttccagg
     3721 cccggcgagc gttccagctt gccggaaaga ttcacgcagg ccactacgaa cgccgccacg
     3781 cccgacttca tgtcgctggt gccgcgcccg tagagcttgc cggcatcggt gtccgcggcg
     3841 aacggaccca ttttccattt cgccgcgccc agcggcacgg tatcgatgtg gccggtgaag
     3901 cacagcggcg ctttgtcgct ggagccgccg atggtcgcga ccaggctggc gcgcttcggc
     3961 gataagcggt gatacgcgac cttgaagccg gcgttctcca gcagcacgcc gagatgccgc
     4021 gcgcactgtt cttcatcgcc cggcgggttg atggtgttca tgcggacgat ctgcctggtc
     4081 agttcgaccg cgtcgatgtg cgctgccacg cctgtgttct cctcctgcct attttcttgc
     4141 gaaatacgct tcacgtactc aggggttctc acgatgctga tccgcgtcgg atcgacagca
     4201 tcgcatgctc tccgcccgac atcgtacccg caagccggcg gacgcgctcc ttcagtcgcg
     4261 ggaaggcttc caacacgtca gccagccgcg ccagcggctt cgcacccggc gtgcgccgat
     4321 agctgctctt cggcgaattc tcgcgcaccg tcacctttgg gaaaccctgc gcccctcggg
     4381 aaggtaagcg atgccgcggc ctatacatcc gccggccgcg ggactaaagt gcgctccgtg
     4441 caccgctgca gcctgacaaa acgtttctgc tacgggagat cttcatgaaa gttgttttcg
     4501 ctttgctccg tgcgttcgtg ctggccctcg cgtttgccgc gagctcagcg gcggcgcagg
     4561 aaaaggtcct gaagttcatc ccgcagtccg atctgcgcat cctggatccg atcacgacca
     4621 ccgcctacat tacccgcaat cacggctaca tggtgtacga cacgttgttc gcgaccaacg
     4681 ccaaattcca gatccagccg cagatggtga acaagtggga gctctcgcgc gacaagctca
     4741 cctacacctt cacgctgcgc gacgggctca agttccacga tggccagccg gtgcgctcgg
     4801 ccgattgcat cgcgtcgatc gagcgctggg gtaagcgcga ttcgctcggc cagaggctcg
     4861 cgcagtcgac cgagtcgtgg acagcggtca acgacaagat cttcaagctc aggctcaaga
     4921 aaccgttccc gctcaccttg gatgccctgg gcaagctgtc ctcgaacgtg ccgttcatca
     4981 tgccggagcg catcgccaag accgatcctt ttcagaacat caccgaggcg atcggctcag
     5041 ggccgttcaa gttcgtgaag gaggagtggg tgcctggcag caaggtggtc tacgtcaaga
     5101 acacggacta cgtgccgcgc aaggaagcac cgtcctgggc cgctggcgcc aaggtcgcaa
     5161 aggtggatcg agtcgagtgg atctatatcc cggattcggc caccgccgcc gcggcgctca
     5221 gcgccggcga ggccgactgg tgggagcagt tgccgcccga tctgatcccg ctgctgcgca
     5281 agaacaagga cgttcgcgtc gagaacatcg acccgctggg ctcgatcggc atgatccgct
     5341 tcaatttcct gcagccccca ttcaacaacc agaagctgcg ccaggcgctg ctctacgtgg
     5401 tcaaccagcg ggattatgtg ctgggaatcg cgggcgacga gaagaacggc cacacttgct
     5461 attcgttctt cacttgcgga acgccgttgt cttccgagat cggcgcagag ccgctgaaag
     5521 gcaagcgcga tttcgataag gccaggaagc tgatccagga gtcgggctac aaaggcgaaa
     5581 aaatcgtgat catttcggcc accgaccagc cgatcgtgca ttcgcagtca ctgctcaccg
     5641 ccgacctgct gcgcaatctg gggctcaacg tggagctgca ggcgggcgac tggggcacgc
     5701 tcatcacccg ccgcacctcc aaagagccag tcgagaaggg cggctggtcg atcttccaca
     5761 cctggtgggt cgggccggac atgatttcgc cggccaacat ggcgatggtc aatctgggcg
     5821 acaagtcgtg gttcggctgg cccgcagatc cgaggctcga ggaattgcgc caagcctggt
     5881 tcaacacgac cgaccccgca tcgggcaaaa aggccgccga tgccgtgcag ctcgaagcct
     5941 tcaacttcgt cccttacatc accacggcgc agttcatcct gcccgcggcc tatcggtcga
     6001 acctgtccgg cataatcgtc gcgcccgtga ctttcctctg gaacgtcgag aagaagtgag
     6061 ggtgccggcc gcccggagcg ccaaatgatc gtctatgttc
//
LOCUS       DASQOP010000272        24494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1142821, whole genome shotgun sequence.
ACCESSION   DASQOP010000272 DASQOP010000000
VERSION     DASQOP010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 24494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24494
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142821"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="VE030_10595"
     CDS             <1..821
                     /locus_tag="VE030_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZF23898.1"
                     /translation="LLLLGLADRRLHELDYPLAVAVIVAVELGIVTVEVVATSAVFIP
                     DTPGLARSWFFTAVATLLVLTYFNLRSRMLSPALTEARLQALQARIRPHFLFNSINAV
                     LSLVRSEPRRAEMALEDLADLFRVLMADNRDLVPLEREVELCRQYLGLEALRLGNRLR
                     VEWQVDKMPGDTLVPPLLLQPLLENAVYHGIEPSMEPGVISIHIFNDRDRVHILLRNP
                     FRKEGSHHTGNKMALANIRERLSLHFDAEASLQTRDSDGQYEVRITLPYVRDGR"
     gene            818..1591
                     /locus_tag="VE030_10600"
     CDS             818..1591
                     /locus_tag="VE030_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HZF23899.1"
                     /translation="MSKHPQSLRVVIADDEAPARSRIRDLLEDCAGSFPIELAGEAAS
                     GRELLALLEETPADVVLLDIRMPEMDGLEVAQHMLKLPEPPSVIFTTAYNDYALKAFE
                     LHAVDYLLKPIRLRRLHDALTRVRAITPLRLDVLQQITPDARTHLSVQERGKVVLIPV
                     PEILYLRAELKYVTIRTAAREYLVEESLSKLEQEFSGRLVRIHRSCLVAKAHISGFEK
                     EGGDSGEAHWVVTLTGLDEKLPVSRRQQHIVKELGRNVL"
     gene            1609..2100
                     /locus_tag="VE030_10605"
     CDS             1609..2100
                     /locus_tag="VE030_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23900.1"
                     /translation="MFLLLVAAAPAGAQGIEALGSRLSDLDRRAYSCDETGTHTLCRA
                     GGKSLEHLGLPALGMTLEYRGDLLNRTTVLFDEARFAEVEGGLTARLGAPEVHDEQLR
                     SGMAGAIVNRVRVWRRDGSVAMLEQFSGKITSSALRYLTSDDYRELMRARDAVRVRGT
                     RDL"
     gene            2206..2676
                     /locus_tag="VE030_10610"
     CDS             2206..2676
                     /locus_tag="VE030_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HZF23901.1"
                     /translation="MAADLGIKEKDRVQIVDGLSKLLADTYTLYLKTHNFHWNVTGPM
                     FQTLHLMFETQYNEMWNNVDLIAERIRSLDAPAPGTYAQFSKLASIKETPGVPKAQDM
                     IKILLEGHEAVTRTARRVFPAAESGNDQPTMDLLTQRMEVHEKTAWMPRSLLEK"
     gene            3022..3966
                     /gene="hemC"
                     /locus_tag="VE030_10615"
     CDS             3022..3966
                     /gene="hemC"
                     /locus_tag="VE030_10615"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HZF23902.1"
                     /translation="MPSAKSPPSRIVIATRESALALWQARHIEGRLGALYPGVETQLL
                     GVTTQGDRMLDISLAKIGGKGLFVKELESALESGEADVAVHSMKDVPMNLPPGFTIAA
                     ITKREDPRDAFVSNDYRDLESLPPGSRVGTSSLRRESQLRARFPLLKVEPLRGNVQTR
                     LRKLDDGRYAAIILAAAGLKRLGLSARIACLLTPEQSLPAVGQGALGIECRAERMDLV
                     AAIAPLDDAPTRWCVEAERAVSRALSGSCQVPLGGYAELTNGRARLRGFVASPDGVRM
                     VYDEIAGSAAGSPADFGTALAERLIAKGAREILAALDG"
     gene            3972..4775
                     /locus_tag="VE030_10620"
     CDS             3972..4775
                     /locus_tag="VE030_10620"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HZF23903.1"
                     /translation="MGGTGSRQPLAGIGVLVTRPEHQSRRLAELIRSLGGEPVLFPAM
                     EIVAQPDDATAPVLKRLHQFDLAIFTSPNAARLAMQRIALAGGLPAGLRVAVLGPGTA
                     AELKKLGVREMITPKADFDSEALLEALSRLVFDGKRVVIFRGQGGRELLGESLRQRGA
                     AVEYVECYRRARPRHGMAELIPLWQQDRLRGCLATSSEIVINLFDMAGEACRPWLCQT
                     PMFVSHPRVAATAFAFGVHKVFVAGMGDEMLASGLETWFARLRRAQPVA"
     gene            4799..5848
                     /locus_tag="VE030_10625"
     CDS             4799..5848
                     /locus_tag="VE030_10625"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF016275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HZF23904.1"
                     /translation="MAEPSSEQQTVVATRPVKPQRRSRLAVLALLLALAASALAAWQW
                     YDARKRFDVMQQDVARRFAEIDTLSRDTRSLAAQSRDELRDVVARLGQIEARMVEVQN
                     QRLALESLYRDLSGSRDVWVLAEVEQTLLIANQQLQLAGNVKAAQIALEAADTRLARM
                     DRPQFTALRRVISQDIERLKAAPYVDVVGMALRLDNVMTAVDSLPLAMEERPRPANDA
                     TAPASGGFWANLWRETMQDLRRLVRIQNAERPEAPLLSPEQAFFLRENLKLRLLGARL
                     ALLARDEASFKADLSAASDWLQRYYDTGSKPGAAALSTVKLLAQSEVGIKLPDISASL
                     DAARNLRMVRERAAR"
     gene            5856..7091
                     /locus_tag="VE030_10630"
     CDS             5856..7091
                     /locus_tag="VE030_10630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HZF23905.1"
                     /translation="MQSDTGGRLMRGLLWVVGLFAVAVGLMLASRYNNGYVLLVLPPW
                     RAELSLNLAILIAVLAVVVAYLGMRAIMSSIAMPGRVRAYLKRRNENRARATFNEALV
                     NFFEGRFGKAEKAAAAALKAGESPALSAILAARAAHGMRAFSTRDGYLAKAAGHEPVE
                     DGMRLIAQAEMLLDERRYHDALDVLKRLPESHTAALRLELRAQQMAKNWDRVLALIPQ
                     LEKRKVFERPVVSQLRRHAIIENLKLKALNGKALREYWDKLSAQDRQEPRIAAAAARC
                     FVDLGACAEAHRIVEDGVERIWDQGLLALYSECLGEDVRQQLERAEKWLQAHPRDAVL
                     LLTLGRLCAHQGLWGKARSYLEASLSIEPTHSAHLQLARLLEREGKLEQAAAHYQKAL
                     EVTLGQLKHYGGGRRQPVL"
     gene            7091..9859
                     /gene="ppc"
                     /locus_tag="VE030_10635"
     CDS             7091..9859
                     /gene="ppc"
                     /locus_tag="VE030_10635"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010396536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HZF23906.1"
                     /translation="MMNAPGQNPAVPDKESPLREDIRLLGRILGDTLREQEGEGVFDL
                     IERTRQNAIRFRRDRDPDARRELETMLNKLDPVRTIAVVRAFSFFSQLANIAEDQHHN
                     RRRRAHLIAGSPPQEGSIALALARGRTAGLPPEKVTAFFRDALISPVLTAHPTEVQRK
                     SILDCQLEIARLLTDRDRMQLTPDELVQNEEALRRVVLTLWQTRVLRELRLTVKDEIE
                     NGLSYYRYTFLRQLPRLYAEIEDLLAQEGFGRAPLSGFLKLGSWIGGDRDGNPHVTHE
                     VMQHALTRHSSTAMDFYFEEIHRLGGELSQSVRIVETTPELERLAAQSPDTSEHRRDE
                     PYRRALTGIYARLAATSSLLDHHVPDRRAVAEAPPYRDSVEFAQDLAVLADSLAANGS
                     ERVARGRLRGLHRAAVIFGFHLAPLDMRQHSGVHEQVVAELFALGARRQGYAALPEAE
                     RRAWLLEELRLPRLLRSPYLSYSEEVGKELAILDTAAGMHRRYGAEALPNYIISKADG
                     VSDLLEVALLLKEVGLLQPGETARLDVNIIPLFETIADLQGCGPIMDQLFSVPYYRAL
                     LASRGNVQEVMLGYSDSNKDGGFLTANWELYKAEVELVKVFARHKIELRLFHGRGGTV
                     GRGGGPSYQAVLAQPPGSVAGQIRITEQGEVIASKYSDPDIGRRNLETLVAATFEATL
                     LEPARMERAEYPEVMQEMSATAFSAYRSLVYETPGFVQFFRTATPITEIADLHVGSRP
                     ASRKKTDRIEDLRAIPWVFSWSLSRMMLPGWYGFGAAVEALAARKGDSGLDLLREMYR
                     DWPFFRTLLSNMDMLLAKSDISIASRYAELVTDAELRERIFSRILDEWHRTLRWLLAI
                     TGQGELLEANPALSRSLRNRSPYIDPLNHLQVELLRRYRSGETQERIRRAILLTINGI
                     AAGLRNSG"
     gene            complement(10016..11050)
                     /locus_tag="VE030_10640"
     CDS             complement(10016..11050)
                     /locus_tag="VE030_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HZF23907.1"
                     /translation="MPHQVTIKPSNHVFTVGEGETVLAAALRESFTLPYGCRNGACGA
                     CKGKILEGKVDYGNPQPHVLADFERKAGLALFCQAKPLTDLVIEAREISGVGELEIRK
                     LPCRVQEMRKVAPDVIVLRLKLPANERLQFLAGQYIDILMKDGKRRSYSMANAPHDDE
                     HIELHVRKMQNGAFTDYVFNKMKEKDILRFEGPLGTFFMREDSDKPIIFVASGTGFAP
                     IKSIIEHAFHLEAPRQMVLYWGGRRPVDIYMGELAARWQQQHDNFSFIPVISEAQPED
                     NWCGRSGFVHRAAMEDFPDMSGYQVYACGAPVVVESAHQDFTTLCKLPEDEFYSDAFT
                     PSVDPKPATA"
     gene            11098..11976
                     /locus_tag="VE030_10645"
     CDS             11098..11976
                     /locus_tag="VE030_10645"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZF23908.1"
                     /translation="MRRLLIIGCGDVGLRMVRALRGRWRIYALTHSESRQALLRAEGI
                     VPVAGDLDRPETLDRLAGLAQDVVHFAPPPGSGTRDTRTANLVRALAKGGSLPQRMVY
                     ISTSGVYGDCGGAVVDETRPARPSSDRARRRLDAERQLRAWGAETGVHVSILRVPGIY
                     AAERLPLARLKAGTPALETEQDPYTNHIHADDLARTVMAALSRGRGGRAYNASDDSRM
                     KMGDYFDLVADQFGLPHPPRISREAAEREIPESALSFMRESRRLANQRLKKELRVRLR
                     YPSVAQGVVAARARLP"
     gene            12029..12373
                     /gene="cutA"
                     /locus_tag="VE030_10650"
     CDS             12029..12373
                     /gene="cutA"
                     /locus_tag="VE030_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HZF23909.1"
                     /translation="MNDVSGAGGEVVVVLTNLPDLATARKLAQLLVEQRLAACVNVLA
                     QCSSVYRWRGAIETATEVPVLIKTVASRYPYVEAAIRANHPYELPEIVSVPVMAGLSE
                     YLDWVAAESAAE"
     gene            12392..12901
                     /locus_tag="VE030_10655"
     CDS             12392..12901
                     /locus_tag="VE030_10655"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD N-terminal
                     domain-containing protein"
                     /protein_id="HZF23910.1"
                     /translation="MNLRIAARFLVELLLAACAGCALASAAADLLAPEQAFRFSATLI
                     EPGLVEVRYRIADGYYMYRDKFRFTTIPQSAGLGDALFPPGKIKDDEFFGRVETYRGE
                     LVIRLPVKSAGGFMLKAVSQGCADAGVCYLPVTQTARLVPAQSSGEAVRSEAYASELL
                     RLQDATPSR"
     gene            12898..13425
                     /locus_tag="VE030_10660"
     CDS             12898..13425
                     /locus_tag="VE030_10660"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZF23911.1"
                     /translation="MKRAGRIALLVLVAVSALATGFWLHPWNWGGVPRSGNVARLMAA
                     SLPDMEGKSQALAQWQGKVMVVNFWATWCQPCLEEIPEFVRMQKKFGSQGLQFVGIAI
                     DHPAKVREFAATHHMNYPVLIGEMEAIELARVSGNELGGLPFTVILDRKGQLIGTELG
                     GLNERKLATIIGSAM"
     gene            13667..14161
                     /gene="aroQ"
                     /locus_tag="VE030_10665"
     CDS             13667..14161
                     /gene="aroQ"
                     /locus_tag="VE030_10665"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HZF23912.1"
                     /translation="MKNASKGGKAASSGPVRPRKVLVLHGPNLNLLGSREPEIYGAET
                     LQGINKRLSALALKSGAKLDSFQSNSEAELVERVQKAGSAGVDFVIINPAAFTHTSVA
                     LRDALAAVRIPYIEVHLSNVHAREPFRRHSYFSDQAVGVICGLGSQGYEYALQCALTH
                     KARK"
     gene            14174..14632
                     /gene="accB"
                     /locus_tag="VE030_10670"
     CDS             14174..14632
                     /gene="accB"
                     /locus_tag="VE030_10670"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HZF23913.1"
                     /translation="MDLRKLKKLIDLVQESGIAELEITEGEEKVRISRAGNAVQTAVM
                     TMPQAPTMTAAAAANAAAAPAAVPEAPAEPEGHLVKSPMVGTFYRSSAPGAKPFVEIG
                     QSVNAGDTLCIIEAMKLLNEIEADQGGVIKSVLVENGQPVEYGQPLFLIG"
     gene            14684..16039
                     /gene="accC"
                     /locus_tag="VE030_10675"
     CDS             14684..16039
                     /gene="accC"
                     /locus_tag="VE030_10675"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HZF23914.1"
                     /translation="MFEKILIANRGEIALRVQRACRELGIKTVAVHSVADADAKYVRL
                     ADESVCIGPAASSESYLNVPAIISAAEVTDAEAIHPGYGFLSENADFAERVEKSGFVF
                     IGPRPDTIRLMGDKVSAKQAMIKAGVPVVPGGEGALPEEPQEIVKVARKIGYPVIIKA
                     AGGGGGRGMRAVHTEAALINAVAMTRQEAKAAFGNPTVYMEKFLETPRHVEIQVLADE
                     HRNAVFLGDRDCSMQRRHQKIIEEASAPLIPIKERVRIGERCAEACRKIGYRGAGTFE
                     FLYEKGEFYFIEMNTRVQVEHPVTEMITGVDIVKTQILVAAGQKLPFRQKDIAFRGHA
                     IECRINAEHPYKFTPSPGRIASWHVPGGPGIRVDSHVYHGYFVPPYYDSLIAKVIAYG
                     DNRDQAIARMRVALSEMIVEGIQTNLPLHQELMLDTAFMRGGTSIHYLEEKLAKYSKV
                     E"
     gene            16064..16957
                     /gene="prmA"
                     /locus_tag="VE030_10680"
     CDS             16064..16957
                     /gene="prmA"
                     /locus_tag="VE030_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HZF23915.1"
                     /translation="MAWLTLELTLDAAQVTALSDALLDAGAISVDVADARAGTEQETP
                     IFQEPGEEAELAYGCNRLIALFDPDIDAVAALSRACAVAGLDPVPEYRTAEVPEQDWV
                     KLTQSQFAPLQVSARLWVVPSWHAAPDGSAINIVLDPGLAFGTGSHPTTRLCLDWLDR
                     HLFPGNSVVDYGCGSGILAIAAAKLGAGRVVGVDIDPQALVTSRYNAEQNRVAATFVG
                     ADGSRPRPADVVMANIFSNPLKILAPLLAQLTVPGGHVVLSGILAPQARAVADAYLEW
                     FDMEPPVEEQGWIRLCGKRVK"
     gene            16968..17780
                     /locus_tag="VE030_10685"
     CDS             16968..17780
                     /locus_tag="VE030_10685"
                     /inference="COORDINATES: protein motif:HMM:NF023334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon and DUF3426 domain-containing
                     protein"
                     /protein_id="HZF23916.1"
                     /translation="MNLYTRCLHCGTTFRVTTQQLQASSGQVRCGQCKQVFDAFATLT
                     AQKPQPATPQRGMPPQAQSGLPPAAPPVERSAPAQIPVRPKERPAAAAPRPDPAASLY
                     EWEFRMPVAPRRTGLWVSLSLLLLAVLALQAAYAFRTELMVLVPQSRKYYERACEWLG
                     CSVALPRLSSFLHIEASDLKAPDPVRPGEIELLVAVRNRAPVEQDYPAFELTLTNSQE
                     QTIARRVFLPVEYLQWTNAMDGLKAGAELPIRLFLDTGELRAAGYRLYLFYP"
     gene            17791..18894
                     /gene="gcvT"
                     /locus_tag="VE030_10690"
     CDS             17791..18894
                     /gene="gcvT"
                     /locus_tag="VE030_10690"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008352518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HZF23917.1"
                     /translation="MLKQTPLNAVHRALNARLVDFGGWEMPLHYGSQIEEHHRVRKDA
                     GMFDVSHMLALDVDGEGAQTFLRQLLANNVDRLMIPGKALYSCMLNDSGGILDDLIVY
                     FLDPRRFRVVVNAATADKDLAWMFSQRARAAAAVTVTPRRDLAMIAVQGPAARAKVWR
                     ALPLSKPATGALGPFFAAEWGAFFFGRTGYTGEDGFEITLPAAEAPGLWQALLDAGVG
                     PAGLGARDTLRLEAGMNLYGQDMDETVTPLECGLKWTVDLESPRDFVGKAALLAAPPR
                     AQMLGLVLLERGVLRAHQEVETRNGHGEITSGSFSPTLDISIALARLPNGVQAGEAAK
                     VKIRDKWLPARTVKYPFVRNGRTLVAGLSSSIP"
     gene            18910..19299
                     /gene="gcvH"
                     /locus_tag="VE030_10695"
     CDS             18910..19299
                     /gene="gcvH"
                     /locus_tag="VE030_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HZF23918.1"
                     /translation="MSIPANLKYTDSHEWARSEADGTVTVGITHHAQDLLGDLVFVEN
                     PALGRKLKKGEECGVVESVKAASDIYAPISGEVVAVNGDLETAPEKINEGAYDAWMFR
                     LKPSNPAELEALLDAAGYQKLVEAEAH"
     gene            19464..20819
                     /gene="gcvPA"
                     /locus_tag="VE030_10700"
     CDS             19464..20819
                     /gene="gcvPA"
                     /locus_tag="VE030_10700"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HZF23919.1"
                     /translation="MPFIPHTEEDIRAMCAAIGVSTIEQLFDEIPQSLKGDGLSGIPA
                     GMNEMQVTRLMHERARRDRPMLNFIGAGAYEHHIPAAVWQIAARGEFYSAYTPYQAEA
                     SQGTLQLLYEYQSMMALLTGLDASNASLYDGASALAEAVLMAVRLHKSARRVLIPAGI
                     NPAYRRVVASIVSNQRIELVGVPYSATDGRVSVATLESLGKADFAGLVVAQPNFFGAL
                     EAVDALTDWAHARGALTIACVNPTALALLKSPGEWGNHGADIAVGEGQPLGVPLANGG
                     PYFGFMACKQALVRQMPGRIVGGTVDVEGQRGYTLTLQAREQHIRRSKATSNICTNQG
                     LAVTAATIYLAMLGAQGLKNVAAQSHANTRLLLEKVTRIPGVQQVFSAPFFHEAVIRL
                     PRPADRVLANMRARGILGGHDVSRDYPELGSALLVCATETKTEADLDAYGAALAAATG
                     A"
     gene            20865..21365
                     /gene="tpx"
                     /locus_tag="VE030_10705"
     CDS             20865..21365
                     /gene="tpx"
                     /locus_tag="VE030_10705"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006765230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HZF23920.1"
                     /translation="MATVTLKGKPVTVAGKLPKKGDAAPDFSLTGKDLADVSLKNYAG
                     KRKVLNIVPSLDTPTCAQSTRVFNQKASGMNNTVVLVISADLPFAMSRFCSAEGLNNV
                     VTLSTFRGRDFHGKYGVDIADGPLKGLTARAVLVLDENNKVVHSELVPEIANEPNYNA
                     ALAALK"
     gene            21441..22889
                     /gene="gcvPB"
                     /locus_tag="VE030_10710"
     CDS             21441..22889
                     /gene="gcvPB"
                     /locus_tag="VE030_10710"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HZF23921.1"
                     /translation="MLVFDQSRHGRRAHAQAPASATTADLPAELLRSELPRLPEVSEL
                     DALRHYTRLSQRNFSIDTHFYPLGSCTMKYNPRACNALAMLPEFLARHPLAPAETGQG
                     YLGCMHELQEILREVTGMEGVSLAPMAGAQGEFAGVAMIRAYHRARGDDARSEIIVPE
                     AAHGTNPATATMCGYTVTEVPTDREGNVDLGALAAAIGPRTAGLMLTNPSTLGVFERT
                     IEKIRDIVHGAGGLLYYDGANLNAILGKVKPGDMGFDVIHMNLHKTFATPHGGGGPGA
                     GPVGVARRLMPFLPLPIVAKEGERFRWLSEDDRPQSIGRMSAFGGNAGVLLRAYVYAR
                     MLGRDGMRRVAEFATLNANYLMIQLRKAGFDLAFDSRRASHEFIVTLKGLKEKTGVTA
                     MDVAKRLLDKGFHAPTTYFPLLVPECLLIEPTETESRETLDAFVAAMKEILDEAHNDP
                     DLVKSAPHTTPVRRLDDVKAARELDLKWTPVK"
     gene            22871..23254
                     /locus_tag="VE030_10715"
     CDS             22871..23254
                     /locus_tag="VE030_10715"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HZF23922.1"
                     /translation="MDSGQVESPHKGKTGLRRIWNALFYSFDGLKAAYRHEDAFRQEV
                     WLAAVLIPLALFLPASGTGKSMMIASVVVVLIVELLNSAVEATVDRVSLEHHRLAKRA
                     KDIGSAAVLFSLINVVVVWLLVLFG"
     gene            23433..>24494
                     /locus_tag="VE030_10720"
     CDS             23433..>24494
                     /locus_tag="VE030_10720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HZF23923.1"
                     /translation="MNGVAMTLACIVDGLQQRNHRIQLIRPRQHATESPARNVHFEEV
                     LSRGVPIPRYQGLRIGLPAKQALFRLWSLKRPDVVHIATEGPLGWSALTAAAKLKLPI
                     STGFHTNFHSYSQHYGVGFLKKPIAAYLRKFHNRAQATLVPTEDLRRELAVQGFENLR
                     VVSRGVDTRLFSPARRSPQLRRDWGAADGEPVALYVGRIAPEKNLKLVVDAFSGMRTR
                     VPRTHLVMVGDGPERAALAARNPDIVFAGMRTGEDLAAHYASADVFLFPSLTETFGNV
                     TLEAMASGLAVVAYDYAAAQQYLAHERSGLLVPFDDSEAFVRLAAALIADPGRAASLR
                     VHARASAERIDWDNVFDDFE"
BASE COUNT         4537 a         7516 c         7993 g         4448 t
ORIGIN      
        1 cattgctgct gctcggactc gccgatcgcc ggctgcacga gctggattac ccgctggccg
       61 tcgccgtgat cgtggccgtc gagctcggca tcgtgactgt cgaggtggtc gcgacctcgg
      121 cggtattcat tccggataca cccggtttgg cgcgcagctg gttctttacc gccgtggcca
      181 cgctgctcgt gctcacctac ttcaatctgc gcagccggat gctgtcgcca gcgctgaccg
      241 aagcgagact gcaggcgctg caggcgcgca ttcgcccgca ttttctgttc aacagcatca
      301 atgcggtcct gtcgctggtg cgcagcgaac cgcgccgcgc cgagatggcg ctcgaagacc
      361 tcgccgacct gttccgcgtg ctgatggccg acaatcgcga tctggtgccg ctcgagcgcg
      421 aagtcgaact gtgccgacag tacctcggcc tggaagcgct gcggctcggc aaccgcctgc
      481 gggtggagtg gcaggtggac aaaatgcccg gggacacgct ggtgccgccg ctgttgctgc
      541 aaccgctgct ggagaacgcg gtctaccacg gcatcgagcc gtcgatggag ccgggggtca
      601 tcagcatcca catcttcaat gatcgcgacc gggtgcacat attgttgcgc aaccccttcc
      661 gcaaggaggg cagtcatcac accggcaaca agatggccct tgccaacatc agggagcgac
      721 tgtcgctgca tttcgacgcc gaagccagcc tgcaaacccg cgacagtgac ggccaatatg
      781 aggtacgcat cacgcttccg tacgtgaggg atggaaggtg agcaagcatc cacaatcgtt
      841 gcgggtcgtg attgccgacg acgaggcgcc ggcgcgaagc cgcatcaggg atctgctgga
      901 agactgcgcc ggcagctttc cgatcgagct cgctggcgag gccgcgagcg gccgcgaact
      961 gctggcgctg ctggaggaaa cccccgccga cgtggtgctg ctggacattc gcatgccgga
     1021 aatggacggt ctcgaagtgg cgcagcacat gctgaagctg cccgagccgc cgtcggtgat
     1081 tttcaccacc gcttacaacg actacgccct gaaggcgttc gagttgcacg cggtcgatta
     1141 tctgctgaag ccgatccggc tgcggcggct gcacgacgcg ctcaccaggg tgcgcgcaat
     1201 aacgccgctg cgcctcgacg tattgcagca gatcactccg gacgcgcgta cccatctttc
     1261 ggtgcaggag cgcggcaagg tggtgctgat tccggttccg gaaatacttt acctgcgcgc
     1321 cgagttgaag tacgtgacca tccgcaccgc cgcgcgcgaa tacctggtgg aagaatcgct
     1381 gtcgaaactc gagcaggaat tctccggccg gctcgtgcgt atccaccgca gttgcctggt
     1441 cgcgaaagcg cacatctccg gtttcgagaa ggagggcggt gacagcggcg aggcgcattg
     1501 ggtggtgacg ctgaccggac tggatgaaaa attgccggtg agccgccgcc agcagcatat
     1561 cgtcaaggaa ctggggcgca acgtcctgtg aatgcacgtc attgcacaat gttcctattg
     1621 ctggttgccg cagcgccagc tggagcgcag ggaatcgagg cgctcggctc gcggctttcc
     1681 gatctcgacc gcagggctta ctcgtgtgac gagaccggta cgcacacact gtgccgggcg
     1741 ggggggaagt ccctcgagca cctgggtttg ccggcgctgg ggatgacgct cgagtaccgt
     1801 ggtgaccttc tgaatcgaac gacggtcctg ttcgatgagg ctcggtttgc cgaagtcgag
     1861 ggcggtctga cggcgcggtt gggtgcgccc gaggttcatg atgaacagtt gcgcagcggt
     1921 atggccggcg cgatcgtcaa ccgggtgcgt gtctggcgca gggacggcag cgtcgcgatg
     1981 ctggagcagt tttccgggaa gatcaccagt tcggcactgc gctatctgac ctcggacgac
     2041 taccgggaac tcatgcgggc tcgcgatgcg gtgcgcgttc gcggtacacg cgatctttag
     2101 acttcggcgc agcgtcgcgc aagtcccgct gaaaaccgcg aagaattgtc ctatgattcg
     2161 aaacgggcgc aggtactctt caacccagag gcgaggaaat caaaaatggc tgctgatctt
     2221 ggcatcaagg agaaggaccg ggtacagatc gtcgatggtt tgtcgaaact gcttgccgac
     2281 acttacacgc tgtacctgaa gacccacaat tttcactgga acgtgaccgg tccgatgttc
     2341 cagacgctgc atctgatgtt cgaaacccaa tacaacgaga tgtggaacaa cgtcgatctg
     2401 atcgccgagc gcatccgctc gctggacgcg ccggcgcccg ggacctacgc ccagttctcc
     2461 aagctcgcgt cgatcaagga aacacccggc gtgcccaagg cccaggacat gatcaaaatc
     2521 ctgctggagg ggcatgaagc agtcacgcgt acggcgcgca gggttttccc ggccgccgag
     2581 agtggcaacg accagccgac catggacctt ctgacacagc gcatggaagt ccatgaaaaa
     2641 accgcgtgga tgccacgaag cttgttggaa aaatagtcac gttggtaatt tgcggtcggc
     2701 ccgaaaacat tggcataagc gctttttcag tggagactta cctgtacagc aggttagtcg
     2761 gaactaaaat acggcttgga tgctcagaag tctattggag aaataatccg actaaaatgc
     2821 atccaagcct tctttcagtg caggctaatg cgcgcagcgc gttatgccgg aactacaaaa
     2881 ccgcgtggat gcttcgcagc ctgctggaga agtgatgcgt tcgtgccgac cggcctaggc
     2941 cgtttgcaaa agtagagctt ttttgggctg ccaccgatat cgcgatggcg gcccgagttt
     3001 gcccctcctg gatccaaacc cttgccgtct gccaagagtc ctccgtcccg aatcgtcatc
     3061 gccactcgtg aaagcgcgct cgctctgtgg caggcgcgcc acatagaggg gcgcctgggc
     3121 gcactctacc ccggagtaga gacacagctg ttgggcgtaa ccactcaggg cgaccgcatg
     3181 ctcgacatct cgctcgccaa gatcggcggg aagggcctgt tcgtgaagga actcgagtcg
     3241 gcgcttgaat ccggcgaggc ggatgtcgcg gtgcactcga tgaaagacgt gccgatgaat
     3301 ctgccgcccg ggttcacgat tgccgccatc accaagcgtg aagacccgcg cgacgccttc
     3361 gtctccaacg actaccgtga cctggagagc ttgccgccgg gaagccgcgt cggcacctcc
     3421 agcctgcggc gcgaaagcca gttgcgcgcg cggtttcctc ttctcaaggt cgagccgttg
     3481 cgtggcaacg tgcagacgcg gttgcgcaaa ctcgatgatg ggcgctacgc agcgatcatc
     3541 ctcgcagccg ccggactcaa gcgcctgggt ctgtcggccc gcatcgcctg cctgcttacg
     3601 cccgagcaga gcctgcccgc agtcggccag ggcgcgctcg gcatcgagtg ccgcgccgag
     3661 cgcatggacc tcgttgcggc gatcgcgccg cttgacgatg cccccacccg ttggtgcgtg
     3721 gaagccgaac gggcggtgag ccgcgcgctc tcgggcagct gccaagtgcc gctgggtgga
     3781 tatgccgagc tgaccaacgg ccgcgccagg ttgcgcgggt tcgtcgccag ccccgatggc
     3841 gtgcgcatgg tgtacgacga gatcgccggt tcggcggcag gttcgcctgc cgatttcggc
     3901 acggcacttg ccgagcgcct cattgccaaa ggtgcgcgcg aaatcctcgc tgcgctggac
     3961 ggatagcaga cgtgggcggg acgggaagca ggcaaccgtt ggcgggcatc ggtgtcctgg
     4021 taacccggcc cgagcatcaa tcgcggcggt tggccgaact gattcgaagc ctcggcggcg
     4081 aaccggtgct gtttccggcg atggagatcg tggcgcaacc ggatgatgct actgcgccgg
     4141 tactgaagcg gctgcaccag tttgacctgg cgattttcac cagcccgaac gcggcgcggc
     4201 tggcgatgca gcgcatcgcg ctagcaggcg gcctgcctgc cggcctgcgc gtggccgtgc
     4261 tgggaccggg aaccgccgcc gaattgaaaa aactcggcgt gcgcgagatg atcacgccga
     4321 aagcggattt cgacagcgaa gctttgctcg aggcgctgtc caggctggtg tttgacggaa
     4381 agagggtggt gatctttcgt gggcagggcg ggcgtgagtt gctcggcgag tcgctgcgcc
     4441 agcgtggggc cgcggtcgag tacgtggagt gttaccggcg cgcaagaccc cggcacggca
     4501 tggcagaact gatacccctg tggcaacagg acagactccg gggctgcctg gccaccagtt
     4561 cggaaatcgt aatcaacctc ttcgacatgg ccggagaagc atgccggccg tggttgtgcc
     4621 agactccaat gttcgtatct catccgcgcg tggccgctac cgcatttgcc ttcggcgtgc
     4681 acaaggtttt cgttgccggc atgggagacg agatgctggc gtcgggactg gagacctggt
     4741 ttgcaagact gcgccgggct cagccagtgg cctgaccgtc ttaattcttc ttggaaatat
     4801 ggccgaacca tcatccgaac agcaaaccgt tgtcgctacg cggcccgtaa agccgcagcg
     4861 acgctcgcga cttgccgtcc tggcgttgtt gctggcgttg gccgcgagcg cgctggccgc
     4921 gtggcagtgg tatgacgcac gcaagcgctt cgacgtaatg cagcaggatg tggccaggcg
     4981 ttttgccgaa atcgacaccc tgagccgcga tacgcggtcg ctggccgcgc agtcgcgcga
     5041 cgagttgcgc gatgtggtgg cacggctcgg gcagatcgag gcgcgcatgg tggaagtcca
     5101 gaaccagcgt ctggcgctcg agtcgctcta cagggatttg tcgggcagcc gcgacgtatg
     5161 ggtgctggcc gaagtcgagc agacgctgct gatcgccaac cagcaactcc agcttgcagg
     5221 caacgtcaaa gccgcgcaga tcgcgctgga ggctgccgat acgcgcctgg cgcgcatgga
     5281 tcgcccgcag tttaccgcat tgcgtcgcgt gatcagccag gatatcgagc ggctgaaggc
     5341 tgcgccatac gtggacgtcg tcggcatggc gctgcgtctg gacaacgtaa tgactgcagt
     5401 ggattcgttg cccttggcca tggaggagcg gccgcggccg gccaacgatg cgactgcacc
     5461 ggcgagcggc ggtttctggg ccaatctgtg gcgcgaaacc atgcaggacc tgcgtcgtct
     5521 ggtgcgcatc cagaacgccg aaaggccgga ggctccgctg ctatcgccgg agcaggcttt
     5581 cttcctgcgc gagaatctga agctgcgtct gctcggggca cggctggcgt tactggcgcg
     5641 cgacgaggcc agcttcaagg ccgatctcag cgcggcttcg gactggttgc aacgctacta
     5701 cgacaccggc agcaagccgg gcgctgccgc gctgtccacc gtgaagctgc tggcgcagtc
     5761 ggaggtcggc atcaagcttc cggacatctc cgcgagcctg gacgcggcgc gcaatctcag
     5821 gatggtgcgc gagcgcgccg cccgctgagc atgacttgca atccgatacc gggggcaggc
     5881 tgatgcgggg tctgttgtgg gtagtcggac tttttgccgt cgccgtcgga ctcatgctgg
     5941 cgtcgcgcta caacaacggc tacgtactgc tggtgctgcc gccatggcgg gccgagctct
     6001 cgctcaatct ggcgattctc atcgcggtat tggcggtcgt cgtcgcctat ctggggatgc
     6061 gcgcgatcat gtcttcgatc gccatgccgg gtcgggtgcg ggcctacctc aagcgccgca
     6121 acgaaaacag ggcgcgtgcc actttcaacg aggcgctggt caatttcttc gaaggccgct
     6181 tcggcaaggc cgagaaggca gccgctgcgg cgctcaaggc gggcgagtcg cccgccctgt
     6241 ctgcaatttt ggcggcgcgc gccgcgcacg gcatgcgcgc cttttccacg cgtgacggct
     6301 atttggccaa ggccgccggc cacgaacctg tcgaagacgg catgcgcttg atcgcgcagg
     6361 ccgagatgct gctggacgaa cgccgctacc acgacgcgct cgacgtgctg aagaggctgc
     6421 cggagagcca cacggccgcg ttgcggctgg aactgcgcgc gcaacagatg gcaaagaact
     6481 gggaccgcgt gctggcgctg atcccgcaac tggaaaagcg aaaggtgttt gaacggccgg
     6541 tggtctcaca gctgcgccgc cacgcgatca tcgagaacct gaagctcaag gcgctgaacg
     6601 gcaaggcgct gcgcgagtac tgggacaagc tgtcggcgca ggaccgccag gaaccgcgca
     6661 tcgccgcagc tgcggcacgg tgcttcgtcg atctaggtgc gtgtgcggag gcgcatcgca
     6721 tcgtcgagga tggcgtcgag cggatctggg accagggact gctggcgctg tattccgaat
     6781 gcctggggga agacgtccgg cagcaactgg aacgtgccga gaaatggttg caggcgcatc
     6841 cgcgcgatgc ggtgctgctg ctcacgctgg gccggctgtg cgcgcaccag ggtttgtggg
     6901 gcaaggcccg cagttacctc gaggccagcc tgtcgatcga gcccacgcat tcggcgcacc
     6961 tgcagctggc gcggctgctg gaacgcgagg gcaagctcga acaggctgcg gcccactacc
     7021 agaaggcgct cgaggtcacg ctcggccagt tgaagcacta cggcggcggg cgcaggcagc
     7081 cggtgctctg atgatgaatg cacccggcca gaacccagcc gtcccggaca aggagtcgcc
     7141 gctgcgggag gacattcgcc tgctcgggcg catcctcggc gacactctgc gcgaacagga
     7201 gggggaagga gttttcgatc tcatcgaacg tacccgtcag aacgccatcc gatttcgccg
     7261 cgaccgcgac ccggacgcca ggcgcgaact cgagaccatg ctcaacaagc tcgacccggt
     7321 gcgcaccata gcggtagtgc gggccttcag tttcttttcg caacttgcca atattgccga
     7381 ggaccagcat cacaatcgcc gccgtcgcgc gcacctgatt gcgggttcgc cgccgcagga
     7441 gggaagcatt gccttggcgc tggcccgtgg gcgcactgcc gggttgccgc cggagaaggt
     7501 caccgccttc ttccgagacg cgctgatctc gccggtgctg accgcgcatc ccaccgaagt
     7561 acagcgcaag agcattctcg actgccagct ggagatcgcc cggctgctca ccgaccggga
     7621 ccgcatgcag ctcacgcccg acgagctggt gcagaacgaa gaggcgctgc gccgcgtggt
     7681 gctgacgttg tggcagaccc gggtgctgag ggagctgcgg ctgacggtga aggacgaaat
     7741 cgagaacggc ttgtcgtact accgttacac cttcctgcgg caactgccgc ggctgtacgc
     7801 ggaaatcgag gacctgttgg cgcaagaagg ttttgggcgc gcgccgctgt ccggtttcct
     7861 gaaactgggc agctggatcg gcggcgatcg cgacggcaat ccccatgtga ctcacgaagt
     7921 gatgcaacat gcgctcaccc gtcattcctc gacggcgatg gatttctatt tcgaggagat
     7981 ccaccggctc ggcggcgagt tgtcccagtc cgtgcgcatc gtcgaaacca cgcccgagct
     8041 cgagcgactg gcggcgcagt cgccggacac ctcggaacat cgccgcgatg aaccctatcg
     8101 gcgcgcgctt accggcatct acgcgcggct cgccgcgacc tcgagcctgc tcgaccatca
     8161 cgttcctgac cgccgcgcgg tcgccgaggc gccgccctac cgggacagcg tggaatttgc
     8221 acaggacctg gcggtgctgg ccgattcgct ggcggccaac ggttcggagc gggtggcgcg
     8281 cgggaggctg cgcggcctgc accgtgcggc agtcatcttc ggttttcatc tggcgccgct
     8341 cgacatgcgc cagcacagcg gcgtgcacga acaagtcgtc gccgaactgt tcgcgctcgg
     8401 ggcgcgccgc cagggttacg ccgccttgcc ggaagcggaa cgccgcgcgt ggctgctgga
     8461 ggaattgcgg ctgccgcggc tgctacgctc gccctacctt tcctacagcg aagaagtcgg
     8521 caaggagctc gcgatcctcg acaccgcggc gggcatgcat cgccgctatg gcgcagaagc
     8581 gttgccaaac tacatcatct ccaaggccga cggcgtcagc gatctgctcg aagtggcatt
     8641 gttgctgaaa gaagtggggc tgctgcagcc cggcgagaca gcgcgtctcg acgtcaacat
     8701 cattccgctg ttcgagacca tcgccgacct gcagggttgc ggcccgatca tggaccagtt
     8761 gttttcggtt ccttactacc gggcgctgct ggcgagccgc ggcaacgtgc aggaggtgat
     8821 gctcggctat tccgacagca acaaggacgg cggtttcctt accgcaaact gggagttgta
     8881 caaggcggaa gtcgaactgg tgaaggtatt cgcgcgccac aagatcgagc tgcgcttgtt
     8941 ccacggccgc gggggtacgg tgggacgcgg cggcggaccc agctatcagg cggtgctggc
     9001 acaaccgccg ggcagcgttg ccggccagat caggatcacc gaacagggcg aggtgattgc
     9061 cagcaagtat tccgatcccg acatcgggcg gcgcaacctg gaaacgctgg ttgcggccac
     9121 cttcgaggct acgctgctcg aacccgcccg catggagcga gccgaatatc ccgaagtgat
     9181 gcaggaaatg agcgccaccg cgttctcggc gtaccggagc ctggtgtacg aaaccccggg
     9241 tttcgtacag ttctttcgga ccgcaacgcc gatcaccgag attgccgacc tgcacgtcgg
     9301 cagccgcccg gcttcgcgca agaagaccga ccgcatcgaa gacctgcgcg cgatcccctg
     9361 ggtattttcc tggtcgctgt cgcggatgat gctgccggga tggtacgggt tcggtgcggc
     9421 ggtggaagcg ctggcggcgc gcaaggggga cagcggcctg gacctgttgc gcgaaatgta
     9481 ccgcgactgg ccgttcttcc gcacgctgct atcgaacatg gatatgctgc tggcgaaaag
     9541 cgacatcagc atcgcctcgc gctacgccga actggtcacc gatgccgagc tgcgcgagcg
     9601 catcttctcg cgcatcctgg atgagtggca ccgcacactt cgctggctgc tggcgatcac
     9661 cggacaggga gagctgctgg aggccaatcc ggcgctgtca cgcagcttgc gcaaccgctc
     9721 gccctacatc gacccgctca accacctgca ggtcgaactt ctgcggcgct accgttccgg
     9781 ggaaacgcag gagcgcatca ggcgcgcgat cctgctgacc atcaacggca tcgccgccgg
     9841 actgcgcaac agcggctagt aaacctgtta gccgccaaga cgccaagagc gccaagaagg
     9901 acaaacgcaa ggttggcaca aagtacacca agcaatgctt cgtgtaaccg tgtgctcttt
     9961 atagttagat ttggggttta ccttggcgct cttggcgtct tggcggcaaa gagccttacg
    10021 cagttgcggg cttgggatcg acggacggag tgaaggcgtc ggagtagaac tcgtcctcgg
    10081 gaagcttgca cagtgtggtg aagtcttggt gagcggattc caccaccacc ggcgcgccgc
    10141 aggcgtagac ctgatagccg ctcatgtccg ggaagtcttc catggccgcg cggtgcacga
    10201 agccgctgcg tccgcaccag ttgtcctcgg gctgcgcttc ggaaatcacc ggaatgaagc
    10261 tgaaattgtc gtgctgctgc tgccagcggg cggcgagctc gcccatgtag atgtcaaccg
    10321 gccggcgccc gccccagtag agcaccatct ggcggggggc ctcgaggtgg aaggcgtgct
    10381 cgatgatgct cttgatcggc gcgaaacccg tgccgctcgc cacgaagatg atcggcttgt
    10441 cggaatcctc gcgcatgaaa aacgtgccca gcggcccttc gaaacgcagt atgtcctttt
    10501 ctttcatctt gttgaacacg tagtcggtga acgcgccgtt ctgcatcttg cgcacgtgca
    10561 gctcgatgtg ctcgtcgtcg tgcggcgcat tcgccatcga gtaggagcgg cgcttgccgt
    10621 ccttcatcag gatgtcgatg tattgcccgg ccaggaactg caggcgctcg ttcgccggca
    10681 gcttcagtcg gagcacgatt acgtcggggg cgaccttgcg catctcctga acccgacagg
    10741 gcagcttgcg gatctccagc tcgcccacgc cgctgatttc gcgcgcctcg atcaccagat
    10801 cggtcaacgg cttggcctgg caaaacagcg ccagccccgc cttcctctcg aagtcggcaa
    10861 ggacatgcgg ctgcggattg ccatagtcca ctttgccttc gaggattttg cccttgcagg
    10921 cgccgcaggc accgttgcgg cagccatagg gaagcgtaaa gctttcgcgc aaggccgcag
    10981 cgagcacggt ctcgccttcg ccaacggtga aaacgtgatt gctgggtttg atcgtgacct
    11041 ggtggggcat ggcagttcgc agacgctgaa ggccgggctc cgggttaaaa tgctggcatg
    11101 agaaggttgt tgattatcgg ctgtggtgac gtcggactgc gcatggtacg tgcgttgcgg
    11161 ggacgctggc gcatttacgc gctgacccac tcggagagcc ggcaggcgct gctgcgtgcc
    11221 gaaggtatcg ttccggtggc tggagatctg gatcgtccgg agacgctcga ccggctcgcc
    11281 ggcttggcgc aggacgtggt gcatttcgcc ccgccgccgg gatcgggaac gcgcgacacg
    11341 cgtaccgcca atctggttcg ggcactcgca aagggcggca gtctaccaca gcgtatggtg
    11401 tacatcagca ccagcggcgt ctatggagac tgcggcggcg cggtggtgga cgagacgcga
    11461 ccggcgaggc cctcgagcga tcgcgcgagg cgtcgcctgg acgccgaacg gcaacttcgt
    11521 gcgtggggtg cggagacagg tgtgcatgtc agcatcctgc gcgtgccggg tatctacgcc
    11581 gccgaacgct tgccgcttgc gcgactgaag gctggcacgc cggccctcga gaccgagcag
    11641 gatccttaca ccaatcacat ccatgccgat gacctcgccc gcacagtgat ggcggcattg
    11701 agtcgcggcc gcggcgggcg tgcttacaac gcttccgacg actcccggat gaaaatgggc
    11761 gactacttcg accttgtcgc ggaccagttc ggtctgccgc acccgccccg gataagtcgc
    11821 gaagccgcgg agcgggagat tccggaaagc gcgctgtcct ttatgcgcga atcccggcgc
    11881 ctcgccaacc agcgcctgaa aaaggagttg cgggtaaggc tgcgctatcc gtcggtcgcg
    11941 cagggcgtcg ttgccgcacg ggcacggttg ccgtgaacaa tgcatccgca gcggctggcg
    12001 cagcgtaacc gcccagtcct ttgccttggt gaatgacgtg tccggcgctg gcggcgaggt
    12061 tgtggtggtg ttgaccaatt tgccggatct tgcgacggcc aggaagctgg cgcagttgct
    12121 ggtggaacag cggctcgccg cctgcgtcaa cgtgctggca cagtgttcgt cggtttatcg
    12181 ctggcggggc gcgatcgaga cggcgaccga ggtaccggtg ctgatcaaaa cggtagcttc
    12241 gcgttatccg tacgtcgaag ccgcgatccg cgccaaccac ccgtatgaac ttcccgaaat
    12301 cgtctctgtc cctgtgatgg ccggtctgag cgagtatctg gactgggtcg ctgccgagtc
    12361 cgccgccgaa taacgccgcc ttttcccccg aatgaatctg cgcatcgccg caagatttct
    12421 tgtcgaactg ctgttggcgg catgcgctgg atgcgcgctg gcgtcggccg ctgccgatct
    12481 gctggcgccg gagcaggcgt ttaggttttc tgccacgctc attgaacccg gcctcgtgga
    12541 ggtgcgttat cgcatcgccg acggctatta catgtatcgc gacaagttcc ggttcaccac
    12601 gattccgcaa tcggccggac tcggcgacgc gctgtttcca cccggcaaga tcaaggacga
    12661 cgaattcttc gggcgggtcg agacctatcg cggcgaactg gtcatcaggc tgccggtgaa
    12721 atcggccggc ggctttatgc tgaaggcggt ttcccagggt tgtgccgatg ccggcgtgtg
    12781 ctacctcccg gtaacgcaaa cggcacggct cgttcctgcg caatcgtcgg gtgaagcggt
    12841 cagatccgaa gcatatgcct cggagttatt gcggttgcag gacgcgacac cgtcccgatg
    12901 aaaagagccg gccggatcgc gctacttgta ctcgtggctg tttcggcgct tgcgaccggt
    12961 ttctggttgc acccctggaa ctggggaggc gtaccgcgtt caggcaatgt ggccaggctg
    13021 atggcggcct cgcttccgga catggaaggc aaatcccagg cattggcgca atggcagggc
    13081 aaggtcatgg tggtgaattt ctgggcgacc tggtgccagc cgtgtctgga ggagatcccg
    13141 gagttcgtcc ggatgcagaa aaaatttggc agccaagggc tgcaatttgt cgggatcgcc
    13201 atcgatcatc cagctaaagt gcgcgaattt gccgccacgc accacatgaa ctatccggtc
    13261 ctgatcgggg aaatggaggc gatcgagctc gccagggtct ccggcaacga actcggcggc
    13321 ctgcctttca cagttatctt ggatcgcaag ggacaattga tcgggaccga gcttggcggg
    13381 ctgaacgagc gaaagctggc cacgattatt gggtcggcga tgtaaatttc gcttcgaacg
    13441 tgcgccacta gagcgctaag ttctgggatc tgcatctaaa aactaggatt cgtgagagtt
    13501 ctggcaattg ccgtgagggt ttgcttggca cggtttcgaa agattgctcc caaagcgtct
    13561 aagccctagg gtcggaaacg cgaaatgcaa gatccaggcc tattgaatgc ggcaaaatcc
    13621 cgctaaaact ggacaaattc gctgaaactg cgtcaaactt gccgccatga aaaacgcctc
    13681 gaagggtggc aaggcggctt cctccggccc tgtacgcccc agaaaggtcc tcgtcctgca
    13741 cggccccaac ctcaatcttc tcggctctcg cgagccggag atctatggag cggaaaccct
    13801 gcagggcatc aataagcgtc tgtcggcgct ggcgttgaaa tcgggcgcca agctcgacag
    13861 ttttcagagc aactcggagg ctgagctggt cgagcgcgtc cagaaagccg gctccgccgg
    13921 cgtcgatttc gtcatcatca atccggcagc ctttacccat accagcgtgg cgctgcgcga
    13981 tgcgctcgcc gcggtacgca ttccctacat cgaagtgcat ctttccaatg tgcacgcacg
    14041 cgagcccttt cggcgccatt cctatttcag cgaccaggct gtgggggtta tctgcggact
    14101 ggggtcgcaa ggctatgagt acgcgttgca gtgcgcgctg acccataaag ccaggaaata
    14161 gggggctgcc tatatggact tgaggaagct caagaagctg atcgacctgg tgcaggagtc
    14221 cggaatcgcc gaactggaaa tcaccgaagg cgaggagaaa gtacggatca gccgtgccgg
    14281 caatgcggta cagactgcgg tgatgacgat gccccaagcc ccgaccatga cggcggcggc
    14341 tgcggcgaac gccgcagcgg cgccggctgc ggtgcccgaa gccccggccg agcctgaagg
    14401 acacctggtg aaatcaccca tggtcggcac cttttatcgc tcctcggcgc cgggagccaa
    14461 gccgttcgtg gaaatcgggc agtcggtcaa tgccggcgat acgctgtgca tcatcgaggc
    14521 aatgaaattg ttgaacgaaa tcgaggccga ccagggtggc gtgattaagt ccgtgctggt
    14581 ggagaacggc cagccggtcg agtatggcca gccgctattc ctgatcggtt gagcatcgac
    14641 cggccggcct ggtttttatc cagtcggcgt gcgtaaagtc tccatgttcg aaaaaatcct
    14701 catagcgaac cgcggtgaga ttgcgctgcg cgtacagcgg gcttgccgtg aactgggcat
    14761 aaagacggtc gcggtgcact cggtggccga cgctgacgcc aagtacgtgc gccttgccga
    14821 cgaatccgtg tgcatcggcc cggccgcctc atcggaaagc tacctgaacg tgccggcgat
    14881 catcagcgcc gccgaagtga ccgacgccga ggcgattcac ccgggctacg gctttctgtc
    14941 cgagaacgcc gattttgccg agcgggtgga aaaaagcggc ttcgtgttca tcgggccgag
    15001 gcccgacacc atccggctga tgggcgacaa ggtgagcgcc aaacaggcaa tgatcaaggc
    15061 cggtgtgccg gtcgtgcccg gaggcgaggg cgcgctgccc gaggagccgc aggaaatcgt
    15121 caaggtggcg cgcaagatcg gctatcccgt gatcatcaag gcggccggcg gtggcggcgg
    15181 acgcggcatg agggcggtgc ataccgaggc ggcgctgatc aacgcggtcg cgatgacccg
    15241 gcaggaagcg aaggccgcct tcggcaatcc aacggtgtac atggagaaat tcctcgagac
    15301 gccccgtcac gtagagatcc aggtgctcgc cgatgagcac cgcaacgcgg tctttctcgg
    15361 cgaccgcgat tgttcgatgc agcgccgcca ccagaagatc atcgaagagg catcggctcc
    15421 cctgataccg atcaaggagc gcgtccgcat cggcgagcgc tgcgccgagg cgtgccgcaa
    15481 gatcggttat cgcggcgcgg gcaccttcga gtttctctac gagaagggcg agttctactt
    15541 catcgagatg aacacgcggg tgcaggttga acatccggtg accgagatga tcaccggcgt
    15601 ggacatcgtg aagacccaga ttctggtagc tgcgggacag aagctgccat tccgccagaa
    15661 agacattgca ttccgcggtc acgcgatcga gtgccggatc aatgccgagc atccctacaa
    15721 attcacaccg tcgccgggcc gcatcgcctc ctggcacgtg ccgggcgggc cggggatccg
    15781 cgtggattct cacgtctacc acggttattt cgtgccgccg tattacgatt cccttatcgc
    15841 caaggtcatt gcctacggcg acaaccgcga ccaggcgatc gcgcgcatgc gcgtcgcgct
    15901 ctcggaaatg atcgtcgagg gcattcagac caacctgccg ttgcaccagg agttgatgct
    15961 ggacaccgca ttcatgcgcg gcggcaccag catccattat ctggaagaga agctcgccaa
    16021 gtacagcaag gtggagtagc ggcggatcca gcggaggtgt ggcatcgcct ggcttaccct
    16081 tgaactcacg ctcgatgccg cgcaggtaac ggcgctgtcg gacgcgcttc tcgatgctgg
    16141 cgccatttcc gtggacgtgg ccgatgcgcg tgccggcacc gagcaggaaa ccccgatctt
    16201 ccaggaaccg ggcgaagagg ccgagctagc ctacggctgc aaccgcctga ttgctctatt
    16261 cgatcccgac atcgacgcgg tcgcagcgct cagccgcgcc tgcgccgtcg ccggcctcga
    16321 tccggtcccc gaatatcgca ccgcggaagt ccccgagcag gactgggtca agctcaccca
    16381 gagccaattc gcaccgctgc aggtgtcggc gcggctgtgg gtcgtgccga gctggcacgc
    16441 cgcgccggat ggttccgcca tcaatatcgt gctcgacccg gggctcgcct tcggcaccgg
    16501 cagccacccg accacgcgac tgtgcctgga ttggctggac cggcatctgt ttcctggcaa
    16561 cagcgtcgtc gactacggtt gcggctccgg cattctcgcc atcgctgcgg cgaaactcgg
    16621 cgccggccgc gtggttggcg tggacattga tccgcaggcg ctggtcacca gccgttacaa
    16681 tgccgagcag aaccgggtag ctgcaacctt cgttggtgct gatggatcgc ggccgcgacc
    16741 ggcagatgtc gtcatggcaa atatcttctc caatccgctc aaaatactgg caccgctgct
    16801 cgcgcagttg accgtgccgg gcggacatgt ggtgctgtcc gggatcctgg caccgcaggc
    16861 aagggctgtt gccgatgctt acctggaatg gttcgacatg gaacccccag tcgaggagca
    16921 aggctggatc aggctatgcg gcaagagagt caaatgaagc agatgccatg aacctgtaca
    16981 cgcgctgttt acactgtggt actactttcc gtgtgaccac ccagcaactg caggcttcca
    17041 gcggccaggt gcgttgcggc caatgcaagc aggtgttcga tgccttcgcg acgctcactg
    17101 cccagaagcc tcagccggca acgccgcaac gtggaatgcc gccccaagcg caatccggat
    17161 tgccgccggc agcgccgccg gtcgaaagat cggcaccagc gcagattccg gtgcggccga
    17221 aggaaaggcc ggcggcggct gcgccgcggc cggatccggc cgcgagcctg tatgaatggg
    17281 aattcaggat gccggtggcg ccgcgccgta ccggtttgtg ggtaagcctg tcgttgctgc
    17341 tgctcgccgt gctcgctctc caggcggcgt acgcgtttcg aaccgagctg atggtgctgg
    17401 ttccgcagtc ccggaagtat tacgagcggg cgtgtgaatg gttgggttgc tccgtggcgc
    17461 tgccgcggct gtcgagcttc ctgcacatcg aggcttcgga tttgaaagcg ccggatccgg
    17521 tccgccctgg cgaaatcgaa ctgctggttg cggtgcgcaa tcgtgctccg gtggagcagg
    17581 actatcccgc gttcgagctg acgctgacca actcgcagga acagaccatc gcgcgccgcg
    17641 tttttcttcc ggtggagtac ctgcaatgga cgaacgcaat ggatggactg aaagcgggcg
    17701 cagagctgcc gatccggctg tttctggaca ccggcgaact gcgtgccgcc ggttaccgtc
    17761 tttacctgtt ctatccttga gctgcccgcg gtgctgaagc agactccgct caacgccgtt
    17821 catcgcgcgc tcaacgccag gctggtggac ttcggcggtt gggagatgcc gctgcactac
    17881 ggctcgcaga tcgaagagca ccacagggtg cgcaaggacg ccggcatgtt cgacgtctcg
    17941 cacatgctgg cgctggacgt ggatggcgaa ggcgcccaaa ccttcctgag acagttgctg
    18001 gccaacaatg tggatcgtct gatgatcccg ggcaaagcgc tttactcgtg catgctcaac
    18061 gactcgggcg gcattctcga cgatctgatc gtgtactttt tggacccgcg acgctttcgc
    18121 gtggtggtga acgccgctac tgcggacaag gatttggcgt ggatgttttc gcaaagggcg
    18181 cgcgccgcag ccgccgtgac ggtcacgccg cggcgtgacc tggcaatgat tgcggtccag
    18241 gggccggcgg cgcgcgccaa agtctggcgc gcgctgccgc tatcgaaacc ggcgaccggg
    18301 gcgctcgggc ccttcttcgc cgcagagtgg ggtgcgtttt tcttcggccg caccggctac
    18361 accggagagg atggcttcga gatcacgctg ccggccgctg aagcgccagg cctgtggcag
    18421 gcgctgctcg atgccggcgt gggcccggcg ggtctgggcg cgcgcgatac gctgcgcctg
    18481 gaggcgggga tgaaccttta cggccaggac atggacgaaa ccgtgacccc gttggagtgc
    18541 ggcctgaaat ggactgtaga cctggaaagc ccgcgcgact tcgtcggcaa ggcggcactg
    18601 ctggctgcgc caccgcgcgc gcagatgctg ggcctggtgc tgctcgagcg cggcgttctg
    18661 cgcgcacacc aggaagtcga gacgcgcaac ggtcacggcg agatcaccag cggcagcttc
    18721 tcgcccacgc tggacatttc gatcgcgctg gcgcggttgc cgaacggcgt acaagccggg
    18781 gaggcggcga aagtaaagat ccgcgacaag tggctgccgg caagaacggt aaaatatcca
    18841 ttcgttcgga acgggcgcac tctggtcgct ggcctttctt cgtcgatccc ttgaatatct
    18901 ggagcattaa tgtcgattcc tgccaatctg aaatacaccg acagccacga atgggcgcgg
    18961 tctgaggccg acgggaccgt gaccgtcggc atcacgcatc acgcacagga cctgctgggc
    19021 gacctggtgt tcgtggaaaa tccggcgctc ggccgcaagc tgaagaaagg cgaggagtgc
    19081 ggcgtggtcg aatcggtgaa agccgcctct gacatctacg cacctatttc cggagaggtc
    19141 gtggcggtga acggcgatct cgaaaccgca ccggagaaaa tcaacgaggg tgcgtacgac
    19201 gcctggatgt tccgtctcaa gcccagcaac ccggccgaac tggaagcatt gctggacgcg
    19261 gcgggttacc agaagctggt cgaggcggaa gcgcattgag ggtgaggcga aaagtcgtga
    19321 ggcgagaggc gtgaggcgag aggcgagagg aataaaccgg cagatggagt gcggtgcttg
    19381 ccggcccgcg ctttttgcgc gtcgcccgtt gcccgttacc cgtcacctag cgctgcgggt
    19441 tcctcacgcc tcgccgaact gaaatgcctt tcatcccgca caccgaagaa gacatccgcg
    19501 ccatgtgcgc ggcgatcggc gtttcgacga tcgagcagct gttcgacgag attccgcaaa
    19561 gcctcaaggg cgatgggttg tcgggcatcc ctgcgggcat gaacgaaatg caggtcacgc
    19621 gcctgatgca tgaacgagcg cgccgggacc ggccgatgct caacttcatc ggggccggcg
    19681 cctacgaaca tcacattccc gccgcggtct ggcagatcgc cgcccgtggg gagttttatt
    19741 ccgcgtatac gccgtatcag gcggaagcga gccaaggcac actgcaactg ctctacgaat
    19801 accagagcat gatggctttg ctcaccgggc tagatgccag caacgccagc ctctacgacg
    19861 gcgcctccgc gcttgccgaa gcggtgctga tggcggtgcg cctgcacaag tcggctcggc
    19921 gcgtgctgat acccgccggc atcaatccgg cctaccggcg cgtggtcgcc agcatcgttt
    19981 ccaatcagcg catcgagctg gtcggggtac cgtactccgc gacggatggc cgcgtgtccg
    20041 tcgcaacact ggagtcgctg ggcaaggcag actttgcggg gctggtggtt gcgcagccga
    20101 acttcttcgg cgcgctcgaa gcggtggatg ccttgaccga ctgggcgcac gcgcgcggcg
    20161 cattgacgat tgcctgcgtc aacccgactg cgctggcgct gctgaaatcc cccggagaat
    20221 ggggcaacca cggtgccgac attgcggtcg gagaaggcca gccgctgggc gtgccgctcg
    20281 ccaacggcgg accctatttc ggattcatgg cgtgcaagca agcgctggtg cggcaaatgc
    20341 cgggccgcat cgtcggcggc acggtcgatg tcgaaggaca gcgcggatac acccttacgc
    20401 tgcaggcacg ggaacagcac atccgccgtt ccaaagccac atcgaacatc tgtaccaatc
    20461 agggcctggc ggtcaccgcc gcgacgatct atctggcaat gctcggcgcg cagggattga
    20521 aaaacgttgc ggcgcaatcg cacgccaata cccggttgct gctggaaaag gtgacacgca
    20581 tccccggcgt gcagcaggtt ttcagcgcgc cgtttttcca cgaggcggtg atccggctgc
    20641 cgcggccggc cgatagggtg ctcgcgaaca tgcgcgcccg cggcatcctt ggcggccacg
    20701 atgtttcccg ggattacccg gaactcgggt cagcgctgct ggtctgcgcc accgagacca
    20761 agaccgaagc cgatctcgat gcctacggcg cagcgctagc cgcagcgaca ggcgcttgaa
    20821 ctgcgcttta cattcacaac cagaattcac tcgaggagag tctgatggca accgtaacgc
    20881 tcaagggcaa acccgtcact gtggcgggca agctgccgaa gaaaggtgat gctgcgcccg
    20941 atttcagcct gaccggcaaa gatcttgcgg atgtgtcgct gaaaaactac gcagggaaac
    21001 gtaaagtgct gaacatcgta ccgagcctgg acacgccgac ctgcgcgcag tccacgcgcg
    21061 tcttcaacca gaaggcttcg ggaatgaaca acacggtggt actggtgatt tcggcggatc
    21121 tgcctttcgc gatgtcgcgc ttctgcagcg ccgaagggct caataacgtg gtgacgctgt
    21181 ccacctttcg cggcagggat tttcacggta agtatggcgt ggacatcgcc gacggcccgc
    21241 tcaaggggtt gaccgcgcgg gcggtgctgg tactcgacga gaacaacaag gtcgttcact
    21301 ccgagttggt gccggaaatc gccaacgaac cgaattacaa tgcagccttg gcggctctga
    21361 aataatgcgg tagcgcacac tgcggatctg cgcgctcacg cgagcgcacg aacgcgcctg
    21421 aatcgccgac ccagaacagc atgctcgttt tcgatcagtc cagacacgga cgccgcgccc
    21481 acgcgcaggc gccggcatcg gcaaccaccg cagacttgcc cgcggagttg ctgcgcagcg
    21541 aactgccgcg cctgccggaa gtatccgagc tcgatgcgtt gcgccactac acccgccttt
    21601 cgcaaagaaa cttctccatc gacacgcatt tttacccgct tggttcctgc accatgaagt
    21661 acaacccgcg ggcgtgcaac gcgttggcga tgctgccgga attcctggcg cgtcatccgc
    21721 tcgcgccggc ggaaaccggg cagggctatc tcggctgcat gcatgaatta caggagatcc
    21781 tgcgggaagt gaccggaatg gagggggtca gcctggcgcc gatggccggt gcgcagggcg
    21841 aattcgccgg tgtcgcaatg atccgcgcct atcaccgcgc gcgcggtgac gacgcccgca
    21901 gcgagatcat cgttcccgaa gccgcgcacg gcaccaaccc ggctaccgca accatgtgcg
    21961 gctataccgt gaccgaagtg cccaccgatc gcgaaggcaa cgtcgatctc ggcgcactgg
    22021 ccgctgcgat tgggccgcgc acggcgggcc tgatgctcac caacccttcc acgctcggcg
    22081 tattcgagcg cacgatcgag aaaatccgcg acatcgtgca tggcgcgggc gggttgcttt
    22141 actacgacgg cgccaatctg aacgcgatcc tcggcaaagt gaagcccggc gatatgggtt
    22201 tcgacgtgat ccacatgaac ctgcacaaga ctttcgccac gccccacggt ggcggcggtc
    22261 cgggagcggg gccggtaggc gttgcacggc ggttgatgcc cttcctgccg cttccgatcg
    22321 tggcgaaaga gggcgagcgt ttccgctggc tttcggagga tgaccgcccg cagtcgatcg
    22381 gccgcatgtc cgcgtttggc ggcaacgccg gggtgctgct gcgcgcctac gtgtatgcac
    22441 gcatgctcgg acgcgatggc atgcggcggg tggcggagtt tgcgacgctg aacgccaatt
    22501 acctcatgat tcagttgcgc aaggccggtt tcgaccttgc tttcgactcg cggcgcgcca
    22561 gccacgagtt catcgtcacc ctgaagggcc tgaaggagaa aaccggcgtc acggcgatgg
    22621 atgtggccaa acgcttgctc gacaagggct tccacgctcc gaccacctac tttcctctgc
    22681 tggtgccgga gtgtttgctg atcgaaccga ccgagaccga atccagggaa acgctggacg
    22741 cctttgtcgc agcgatgaag gaaatcctcg acgaggcgca caacgatccg gaccttgtaa
    22801 aatccgcgcc ccacaccacc ccggtgcgcc ggctcgacga cgtcaaggcc gcgcgcgagc
    22861 tcgacctcaa atggactccg gtcaagtaga aagcccgcac aaaggcaaga ccggattgcg
    22921 gcgcatctgg aacgcgctgt tctattcgtt cgacggcctc aaggcggcgt accggcacga
    22981 agacgcgttc cgccaggaag tgtggctcgc tgcggtcctg atcccgctgg cgttgttcct
    23041 gccggcgtcc ggaacgggca agtcgatgat gatcgcctcg gtggtggtgg tgctgatcgt
    23101 cgagctgctc aattccgcgg tggaagcgac ggtggaccgg gtatcgctcg agcaccatcg
    23161 gctcgccaag cgcgccaagg acatcggcag cgccgcggtc ctgttttccc tcatcaatgt
    23221 cgtggtggtc tggctgctgg tgctgttcgg ctgattctcg ctccagagcc ggtgccgtag
    23281 tcacacaatc gtcatcgaat cgtcatcgca gtttcgtcgt ggcacggcaa agttcgctgt
    23341 gcatgaacga taatgcgttt gtttttcagg agcttccagc cacatcttgg tctttgcggg
    23401 tagccgttgt cacagagact tatccgcccg aagtgaacgg tgtagcgatg acgctcgcgt
    23461 gcatcgtaga cgggctgcag cagcgtaatc atcgaatcca gttgatccgc ccgcgccagc
    23521 acgcgaccga gtctccagcg cgcaacgttc attttgagga agtgctcagt cgcggcgtcc
    23581 cgatcccgcg ctatcaagga ctcaggatcg gcctgccggc gaaacaggcg ttgttccgtc
    23641 tctggtcgct gaagcggccc gatgttgtgc acatcgctac cgagggaccg ctgggctggt
    23701 cggctctgac cgcggcagcc aaactgaagc ttccgatttc caccggtttt cacaccaact
    23761 tccacagtta cagtcagcac tacggcgtag gttttctgaa gaagccgatc gccgcctacc
    23821 tgcgcaagtt ccacaacagg gcgcaagcca cgctggtgcc gaccgaagat ttgcgccgcg
    23881 agctggcagt gcaggggttc gagaacttgc gcgtggtatc gcgcggggtc gatacccggc
    23941 tgttctcgcc ggcacgcaga agcccgcaat tgcgccgcga ctggggtgcg gcggacgggg
    24001 agcccgtggc actctatgtc ggcaggatcg cccccgaaaa aaatctcaaa ctggtggtgg
    24061 acgcgttctc aggcatgcgc acgcgtgtac cgcggacaca cttggtcatg gtcggggacg
    24121 gcccggaacg ggcagcgctt gccgctcgta atccggacat cgtattcgca ggcatgcgca
    24181 ccggagagga tctggccgcg cattatgcct ctgctgacgt attcctgttc ccgagcctta
    24241 ccgaaacttt cggcaacgtc acgctcgaag ccatggcaag cggcctggcg gtggtggcgt
    24301 acgactacgc ggccgcgcaa caatacctcg cccacgaacg cagcggttta ctggtgccgt
    24361 tcgatgatag cgaggcattc gtccggctgg cagccgcgct tattgccgat cccgggcgcg
    24421 ccgcgtcgtt gcgggtgcac gcgcgtgcaa gcgccgagcg tatcgactgg gacaacgtgt
    24481 tcgacgattt cgag
//
LOCUS       DASQOP010000273        13271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_332748, whole genome shotgun sequence.
ACCESSION   DASQOP010000273 DASQOP010000000
VERSION     DASQOP010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13271
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332748"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1640)
                     /locus_tag="VE030_10725"
     CDS             complement(<1..1640)
                     /locus_tag="VE030_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HZF23924.1"
                     /translation="MPFRNGGRILVDQLQLHGTDTAFCVPGESYLAVLDALFEARERV
                     RLVVCRQEGGAAFMAEAYGKLTGKPGICFVTRGPGATNASIGVHTAFQDSTPMILFIG
                     QVGSDVVEREAFQEMDYRRMFGQMAKWVAQIDRTDRIPELVSHAFHTAVSGRPGPVVL
                     ALPEDMLTGQAVIADAKPYKRVKPHPGVADMERLRDMLVQAHKPMVVLGGGDWSAQAC
                     NDIRAFIESNNVPAGCSFRRQDLLDNRHPNYCGEVGIGINPLLGERIRSADLILAIGP
                     RLGEMTTGGYTLFPIPRPLQRLVHVHPGGEELGRVYQAELMINSGMAEFAQAAANLPS
                     VDSSRWRDWTHRAREDYLKNLKPGPMSGDLDLGEVMVFLRGRLPENAIITNGAGNYSG
                     WVQRFYQYTGFRTQLGPTNGAMGYGVPSGIAAKVVHPDRVVVSFSGDGCFLMNGQELA
                     TAAQYRLPVVFVVINNGMYGTIRMHQEREYPARVYATALDNPDFAALARAYGLHGETV
                     QRTADFAPAFERAVTSGKPALLELRIDPEAITTRTSLTS"
     gene            1839..3572
                     /locus_tag="VE030_10730"
     CDS             1839..3572
                     /locus_tag="VE030_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HZF23925.1"
                     /translation="MVWSQVYDPLGNAWLSTLCAAIPIIVLLGSLAFFRIAAHWAAIL
                     GLVSALFIAIVVYGMPTATAGGAALNGALFGLLPIGWIVLNIIFLYQLTKQKGYFKVL
                     QDNIANVTEDRRLQLLLIAFAFGAFFEGAAGFGTPVAVTGAILIGLGFSPLAASGLSL
                     IANTAPVAYGALGTPVITLAAVTGLDVNALSAMVGRQLPFFSVLVPFWLIWAFAGWRG
                     MIAVWPAILVTGVSFAIPQFLISNLHGPMLVDVGSAVISMACLVTFLRFWKPKQIWTS
                     TALKGHQRDSGIADVAPTKFEQHPSGLVIRAWIPWLILSVFVFAWGFQTTKNFLDGKT
                     TETITVDGQTKQKVSYAFTRVEIPIANLHEKVEKVPPVVAKPVKEGAKYNFNWVSATG
                     SGIFLAAVVAGLFMGYSIGGLFRQYWMTIKLVKFSLLTIAAMLALGFVTRYSGTDATM
                     GLAFAHTGVLYPFFGTLLGWLGVALTGSDTSSNVLFGGLQKITSEQLGLNPILMTSAN
                     SSGGVMGKMIDAQSIVVASTATRWYGHEGDILRYVFFHSVVLAMLVGGLVMLQAYVYP
                     FTLLQIFPATP"
     gene            complement(3642..5744)
                     /gene="pnp"
                     /locus_tag="VE030_10735"
     CDS             complement(3642..5744)
                     /gene="pnp"
                     /locus_tag="VE030_10735"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HZF23926.1"
                     /translation="MNVIRKTINYGRHTLTLETGEVARQAHGAVMVNMDDTVVLVTVV
                     GNKDAKPGQDFFPLTVDYQERTYAAGKVPGGFFRREGRPSEKETLTSRLIDRPIRPLF
                     PEGFFNEIQVIATVISSNNEVDSDIPAMIGASAALAISGIPFNGPIGGARVGYLNGEY
                     VLNPTATELKSSRLNLVVAGTKQAVLMVESEAMELSEDVMLGAVVFGHQQMQAVIDTI
                     NELADEAGKPVWDWAPPQKDGSISERIAQLAEEELREAYRLRQKQARSEKVEQIRAKV
                     LTEVAPEGSDSAHVNLVKSIFSDIESKIVRSQILNGEPRIDGRDTRTVRPITIRAGVL
                     PRTHGSALFTRGETQALAVATLGTVRDEQIIDALQGEYRERFMLHYNMPPYATGETGR
                     VGTPKRREVGHGRLAKRALLAVLPAPEEFAYSMRLVSEITESNGSSSMASVCGGCLAL
                     MDAGVPLKAHVAGIAMGLIKEGNRFAVLSDILGDEDHLGDMDFKVAGTESGITALQMD
                     IKIQGITKEIMNVALLQAREGRMHILGLMRQTLATPRSELSAYAPRMITIKIKPEKIR
                     DVIGKGGAVIRAITEETGTTIDINDDGTVTIACVSSEGGEAAKRRIEEITAEVEVGRV
                     YDGTVLKLLDFGAIVSVLPGKDGLLHISQIANERVNSVADHLKEGQAVRVKVLEADEK
                     GRLRLSMKAVAEEAKAAG"
     gene            complement(5897..6166)
                     /gene="rpsO"
                     /locus_tag="VE030_10740"
     CDS             complement(5897..6166)
                     /gene="rpsO"
                     /locus_tag="VE030_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HZF23927.1"
                     /translation="MAITTSQKAQILQEHQRAKADTGSPEVQVALLTARINDLTDHFK
                     AHVKDHHSRRGLLKMVSRRRKLLDYLRQASSERYRSLLDRLGLRK"
     gene            complement(6229..7152)
                     /gene="truB"
                     /locus_tag="VE030_10745"
     CDS             complement(6229..7152)
                     /gene="truB"
                     /locus_tag="VE030_10745"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HZF23928.1"
                     /translation="MIHGVLLLDKPAGLTSNAALQRVRRLFGRIKAGHTGTLDPLATG
                     LLPICLGEATKFSYSMLDADKSYEAVIRLGFVSSTGDAEGAIREAGVPDFGVDKLREV
                     VRVFTGPILQRPPMHSALKKDGRPLYAYARAGIEVERSVRNVLIKSLEIIDLKKNMLS
                     ITVACSKGTYIRVLAQDIGEALGCGGYLSALRRTRIADLDVASAIGLEELEKSGPVVG
                     ATRVLPVDLLVSAYPALRLDSAQVKRVSTGLEVRPAVEVVQRGPVRLYGPTGVFLGLG
                     EGRSGGIVAPKRMLANPAAVQEKPADARLTP"
     gene            complement(7196..7579)
                     /gene="rbfA"
                     /locus_tag="VE030_10750"
     CDS             complement(7196..7579)
                     /gene="rbfA"
                     /locus_tag="VE030_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HZF23929.1"
                     /translation="MAKDPSRLGRIADQIQRDLAGLLRSELKDPRVGMITLTGVEVSN
                     DYRHAKIFFTTLGGPEVVSQTQQGLQHASGFLRSQLAGGLKLRIVPELHFVHDESVER
                     GVRLSKLIDEAVASPPSVKPGKDQD"
     gene            complement(7764..10322)
                     /gene="infB"
                     /locus_tag="VE030_10755"
     CDS             complement(7764..10322)
                     /gene="infB"
                     /locus_tag="VE030_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HZF23930.1"
                     /translation="MAQMNVSQFAKELGLPSALLLEQLQAAGVGKALAEDTVLTEQDK
                     TQLLDYLRRVHGAKDTKNKITLTRRQTTEIKKADSTGRARTIQVEVKKKRVLVKRDAA
                     AAEAPAPVAETAPPAAIDAEQTALREEEARKQAELIARQSAEAVQKQQRRRKTAKDSV
                     PAAVAESEKPVVAAAPQPAEGPAAKKLTTEGTLHKPATKPGDKPAKPKTAKKSETAWK
                     DESARRRGLKTRGDDSRGGQGWHARMGRSPKHKSEVVTTDQSFNAPTEPIVREVLVPE
                     TITVAALAQKMSVKAAEVIKVLMKLGSMVTINQVIDQETAMIVVDEMGHIAKRAKLDD
                     PESFLSEEAEKHDVVAEPRAPVVTVMGHVDHGKTSLLDYIRRTRVASGEAGGITQHIG
                     AYHVNTPRGMITFLDTPGHEAFTAMRARGAKVTDLVVLVVAADDGVMPQTIEAIHHAK
                     AAKVPLVVAVNKVDKPEANPEKIRQQLAGEQVVPEEWGGDTMFQDVSAKSGKGIDELL
                     ERILLQAEVLELRAPKNVPAHGVVIESRLDKGRGPVATILVQSGTLKRGDVVLVGAVF
                     GRVRAMLDEAAHPVPEAGPSIPVEVQGLSDVPIAGEEMVVLGDERKAREIALFRQGKF
                     REVKLAKQQTAKLEGMFDQMSVGQAKALSLIIKADVQGSYEALSQSLEKLSTAEVKVN
                     IVHAGVGAITESDVNLALASNAVIIGFNTRADTMARKLVAAHGIELRYYNVIYEAVDE
                     IKAALSGMLTPDKKESTIGMVEIRQVFKISKVGTVAGCYVLEGLVRRGSQVRVLRDNV
                     VIFSGELDSLKRFKDDVREVKAGFECGLSVKNFDDLKVGDQLEVYEVVEIARTL"
     gene            complement(10329..11813)
                     /gene="nusA"
                     /locus_tag="VE030_10760"
     CDS             complement(10329..11813)
                     /gene="nusA"
                     /locus_tag="VE030_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HZF23931.1"
                     /translation="MSRELLMLVDALAREKNVEKDIVFGALELALASATKKRFKDDVD
                     VRVEIDRETGEYKSFRRWQIVADNDHEIPAQQIALTDAHERDPNLQINDFVEEPLEPV
                     EFGRIGAQTAKQVILQKIRDAEREQILNDFLQRKEFIVTGAIKRMERGNAIIESGRIE
                     AALPKDQMIPKENLRIGDRVRAFLLKVDRNNRGPQLILSRTVPEFLIKLFEMEVPEIE
                     EGLLEIKAAARDPGSRAKIAVKSNDPRIDPIGTCVGMRGSRVQAVTAELAGERVDITL
                     WSPDPAQFVINALAPAEVSKITVDEESHSMDVVVDEENLAQAIGRNGQNVRLASELSG
                     WELNIMTEEASQKKNEEESATIRKLFMERLDVDEEVAGILIQEGFTTLEEVAYVPINE
                     MLEIESFDEDTVNELRSRARNALLVEAIASEEHVENVASDLLTLEGMDNETARVLAAK
                     GVTTQEDLADLAVDDLVELTGIDAERAKQLIMTARAPWFAASQG"
     gene            complement(11829..12242)
                     /gene="rimP"
                     /locus_tag="VE030_10765"
     CDS             complement(11829..12242)
                     /gene="rimP"
                     /locus_tag="VE030_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HZF23932.1"
                     /translation="MLEKTVGGLGYELVDVELANHGRLMRIFIDKVGGVNVDDCAAVS
                     NHLTRLFAVENVDYDRLEISSPGLDRPLKRAKDFERFSGEHVQVKLRIPINGRKRVAG
                     VLQNVREDGFQLDVDGELITIAFADINKARLVPNI"
     gene            complement(12949..13062)
                     /gene="rrf"
                     /locus_tag="VE030_10770"
     rRNA            complement(12949..13062)
                     /gene="rrf"
                     /locus_tag="VE030_10770"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         2370 a         4156 c         3867 g         2878 t
ORIGIN      
        1 gatgtgaggc tggtgcgcgt ggtgatggct tccggatcga tccgcagttc cagcaacgcc
       61 ggtttgcccg acgtcaccgc ccgttcgaat gccggtgcga agtccgcggt cctctgcacc
      121 gtctcaccgt gcaatccgta ggcgcgcgcc agcgcggcaa agtccggatt gtcgagcgcg
      181 gtcgcgtaca cccgcgcggg atactcgcgt tcctggtgca ttcggatggt gccgtacatg
      241 ccgttgttga tcacgacgaa cacgaccggc agccgatact gcgcggctgt agccagttcc
      301 tgcccgttca tcaaaaagca gccgtccccg gaaaaactca ccaccacgcg atcggggtgc
      361 accaccttgg cggcgatacc cgaaggcacg ccgtaaccca tcgcgccgtt ggtgggaccc
      421 agttgggtac ggaaaccggt gtactgatag aaacgctgta cccagcccga gtaattgccc
      481 gcgccgttgg tgatgatggc gttttccggc aagcggccgc gcaggaagac catcacctcc
      541 cccagatcaa gatcgccgga catcgggcca ggcttgagat tcttcaggta atcctcgcgg
      601 gcccgatggg tccagtcgcg ccagcgcgag gaatccacgg atgggaggtt cgctgccgct
      661 tgcgcaaatt ccgccatccc ggagttgatc atcagttcgg cctgataaac ccggcccaac
      721 tcttcgcccc ctggatgcac gtgcacgagg cgttgcagcg gacgtgggat cgggaacaat
      781 gtgtatccgc cggtggtcat ctcgcccagc cgcggaccga tcgccaggat caaatcggcg
      841 ctgcgtatgc gttccccgag caagggattg atgccgatgc ccacctctcc gcagtagttg
      901 ggatgccggt tgtcgagcag atcctgcctg cggaaactgc atcccgcagg cacgttgttg
      961 gattcgatga aggcgcgaat gtcattgcac gcctgcgccg accagtcgcc gccgccgagt
     1021 acgaccatcg gtttatgcgc ctgcaccagc atgtcgcgca atctctccat gtcggccacg
     1081 cccggatgcg gtttcaccct cttgtaaggc ttggcatccg cgatcaccgc ctggcccgtc
     1141 agcatgtctt caggcagtgc cagcaccacc ggaccggggc gccccgacac cgcggtatga
     1201 aacgcgtggc tgaccagttc ggggatccga tcggtgcggt cgatctgcgc cacccatttc
     1261 gccatttgcc cgaacatgcg acggtagtcc atttcctgaa acgcctcgcg ctcgactaca
     1321 tcgctgccga cctggccgat gaacaggatc atcggggtcg aatcctggaa tgcggtgtgc
     1381 acgccaatgc tggcgttggt ggcccccggc ccgcgggtga cgaagcagat tccgggtttt
     1441 ccggtgagct tgccgtaggc ctcggccatg aacgctgcac cgccctcctg ccggcagacg
     1501 accaggcgca cgcgctcacg cgcctcaaag agcgcatcca gcaccgccag ataactttcg
     1561 cccggaacgc agaatgcggt gtcggttccg tggagctgca actgatccac cagaattcgg
     1621 ccgccgttcc gaaaaggcat gttttcgttc atcgcactcg tcggtgtagt ctgagcgccg
     1681 gattataccc aaggcatccg catatccaga tccacaattc gagccgcgtg tcggcgtgat
     1741 cgatttggat ataatgggct ggatttcaaa gcattaggcg gagcgttcgc acccgacggc
     1801 cgggcgacgt actgacaaca acagggaggt cacaactcat ggtctggtcc caggtttacg
     1861 atccactcgg aaatgcctgg ctttcaacgc tgtgcgcagc gattcccatc atcgtactgc
     1921 tcggcagcct ggcgttcttc aggatcgccg cgcattgggc tgcgattctt gggctggtaa
     1981 gcgctctgtt catcgccatc gtcgtctacg gcatgcccac ggcgacggcc ggtggggctg
     2041 cactcaacgg tgcgctgttc ggtctgctcc ccatcgggtg gatcgtgctg aacatcattt
     2101 ttttgtatca actcacgaag caaaagggtt acttcaaggt cctgcaggac aatatcgcca
     2161 acgtaaccga ggacaggcgg ttgcaactgc tgttgatcgc cttcgccttc ggcgcattct
     2221 tcgaaggcgc tgctggattc ggcacaccgg tcgcagtgac aggggcgatc ctcattgggc
     2281 tgggcttctc gccgctggcc gcgtcgggcc tgtctttgat tgcgaacacc gcgccggtgg
     2341 cctatggagc gcttggtacg ccagtgatca cgcttgcggc cgtgacgggc ttagacgtga
     2401 acgccctgtc cgccatggtg ggacgccagc tcccgttctt ctcggtgctc gttccattct
     2461 ggctgatctg ggcgttcgcc ggctggcgcg ggatgatcgc cgtatggcct gccatcctcg
     2521 tgactggcgt cagcttcgcg atcccgcagt ttctcatctc gaacctgcac ggcccaatgc
     2581 tggttgacgt gggttcggcg gtgatatcca tggcctgcct ggtgaccttc ctcaggttct
     2641 ggaagccgaa gcagatctgg accagcaccg cactcaaagg tcaccagagg gacagcggga
     2701 tcgcagatgt cgcgccaacc aagttcgagc agcatccctc cggcctggtg atccgtgcct
     2761 ggatcccgtg gttgatcctg agcgtgttcg tattcgcttg gggcttccag accacgaaga
     2821 atttcctgga cggcaaaacc acggagacca tcactgtcga cggacaaacg aagcagaagg
     2881 tgagctatgc cttcactcga gttgagatcc ccattgccaa tttgcacgag aaggtcgaga
     2941 aggtaccgcc ggtggtggcc aagcctgtca aggagggcgc caagtacaat ttcaattggg
     3001 tgtccgccac cgggtccggc atctttctcg ccgccgtagt agccggctta ttcatgggtt
     3061 attccatcgg tggactattc cgccagtatt ggatgacgat caaactggtg aaattctcgc
     3121 tgctcaccat cgcggcgatg ctggccttgg gattcgtaac gcgctactcc ggcactgacg
     3181 cgacgatggg gctcgcattt gcccacaccg gcgtactcta cccgttcttc gggacactgc
     3241 tcggctggct gggcgtggcg ctgaccggtt ccgacacgtc gtcaaacgtt ctgttcggtg
     3301 gcctgcagaa gataacctca gagcagctcg gcttgaatcc gatcctgatg acctcggcca
     3361 atagctccgg cggcgtgatg ggcaagatga tcgacgcgca gtccatcgtc gtggcttcca
     3421 ccgcgacccg ctggtacggc cacgagggcg acatcctgcg ctacgttttc ttccacagcg
     3481 tggtactggc gatgctggtc ggcggcctgg tgatgctgca ggcctacgtc tatccgttca
     3541 cgctgctgca gatctttcct gcgacaccat agcgctgtcg gcaacgcagc caacaaaaag
     3601 cccaccgctt gcggtgggct tttttcgtcc gttgaatcct gctaaccggc ggcctttgcc
     3661 tcttcggcga cggccttcat cgacagccgc agccgccctt tctcgtcggc ctcgaggacc
     3721 ttgacccgca cggcctgacc ctccttgagg tggtcggcaa cgctgttgac gcgctcattg
     3781 gcaatctggg agatgtgcag caagccgtcc ttgccgggca gcacgctcac gatcgcgccg
     3841 aaatccagga gcttgagcac ggtgccgtcg tagacgcggc cgacttcgac ttccgcggta
     3901 atctcctcga tgcggcgttt ggccgcctcg ccgccctccg aagaaacgca ggcgatggtc
     3961 actgtaccgt cgtcgttgat gtcgattgtg gtgccggtct cttcggtgat cgcgcgtatc
     4021 accgcaccgc ccttgccgat cacgtcccgg attttctccg gcttgatctt gatggtgatc
     4081 atgcgcggtg cgtaagcgga gagttccgag cgcggggtgg ccagcgtttg ccgcatcaag
     4141 ccgagaatgt gcatccgccc ttcgcgcgcc tgcagcagcg cgacgttcat gatttccttg
     4201 gtgatgccct ggatcttgat gtccatctgc agggccgtga tacccgattc ggtcccggcg
     4261 accttgaaat ccatgtcgcc gagatggtcc tcgtcaccga ggatgtcgct cagcaccgcg
     4321 aagcggttgc cctccttgat caggcccatg gcgatgcccg caacatgcgc cttgagcggc
     4381 accccggcat ccatcagcgc cagacagccg ccgcacaccg atgccatcga actcgatccg
     4441 ttggactcgg taatctcgga aaccagtcgc atcgaatagg caaattcctc gggagccggc
     4501 agtaccgcca gcagcgcgcg cttggcaagc cgtccatgcc cgacttcgcg ccgcttcgga
     4561 gtgcctacac ggccggtttc tccggtcgcg taaggcggca tgttgtagtg aagcatgaag
     4621 cgctctctgt actcgccctg cagcgcgtcg atgatctgtt cgtctcgtac ggtgcccagc
     4681 gtggccaccg ccagggcctg cgtttcgccg cgggtgaaca gcgccgaacc atgggtgcgc
     4741 ggcagcacgc cggcgcggat tgtgatcggc cgcacggtgc gggtatcgcg tccgtcgatg
     4801 cgcggctcgc cgttgagaat ctggctgcgc acgattttcg actcgatatc gctgaaaatc
     4861 gacttcacca ggttgacgtg cgcgctgtcc gagccttcgg gcgccacttc ggtcaaaacc
     4921 ttggcacgga tctgctctac cttctcgctg cgcgcctgtt tctgccgcag gcgatatgcc
     4981 tcacgcagtt cctcctcggc aagctgggcg atacgttcgc tgatgctgcc gtccttctgg
     5041 ggaggggccc aatcccacac cggcttgccg gcttcgtcgg caagttcgtt gatggtatcg
     5101 atgaccgcct gcatctgctg gtggccgaac acgactgcgc ccagcatcac gtcttcggaa
     5161 agctccatcg cctcggactc gaccatcagc accgcctgct tggtgccggc cacgaccagg
     5221 ttcagccggg aggatttgag ttcggttgcc gtagggttga gaacgtattc gccgttaagg
     5281 tagcctacgc gcgcaccacc gatcggtccg ttgaagggaa tacccgagat cgccagcgca
     5341 gcggaagcgc cgatcattgc cgggatgtcg gagtccacct cgttgttcga ggaaatgact
     5401 gtggcaatga cctggatttc gttgaagaag ccctccggga acagcgggcg gatgggacgg
     5461 tcgatcaggc gcgaggtgag cgtttctttt tccgatggcc tgccctcgcg cctgaaaaat
     5521 ccgccgggga ccttgccggc tgcgtaggtt cgctcctgat aatccacggt cagcgggaag
     5581 aaatcctgtc ccggcttggc gtccttgttg ccgacgacgg tgaccagcac cacagtgtcg
     5641 tccatgttta ccatgaccgc gccgtgagcc tggcgtgcaa cttcgccggt ttcgagcgtc
     5701 agcgtgtggc gcccgtagtt gatggttttc ctgattacgt tcaagagggt tccctctgat
     5761 gctgaattgc gaaggagcgg ggaccgctcc ttgacgcggt tgatcgcttc ggaatttccg
     5821 gtcggcgcat cgctgccagt ccggagtacg ggaaatgcct aagacttgcc cggagccacc
     5881 gaaggaaatg aattctttac ttgcggagcc cgagacgatc aagcagcgag cggtaccgct
     5941 cgctgctcgc ctggcgcagg tagtccagca acttgcggcg gcggctcacc attttcaaca
     6001 gcccgcggcg ggaatgatgg tcctttacgt gggccttgaa atgatcggtc aggtcgttga
     6061 tgcgcgcggt aagcagcgcg acctgcactt cgggagagcc ggtgtcggcc ttggcccgct
     6121 gatgttcctg caaaatctga gccttttggc tcgtcgttat cgccattacc ctgtctccga
     6181 aagaaaggcc gacattctac ccgcggaatc cggggtttca aagcgtttct agggggttaa
     6241 tcgcgcatct gccggctttt cctgcaccgc cgccgggttg gccagcatgc gtttcggggc
     6301 gacgatgccg ccggagcggc cttcacccag tcccagaaaa actccagtcg gaccatacaa
     6361 cctgacgggg ccccgctgca cgacctcaac ggcgggccgc acctcgaggc cggtgctgac
     6421 gcgtttgacc tgggcgctat cgagacgcaa tgccggatag gcgcttacca gcaggtcgac
     6481 gggcagcaca cgggtggctc cgaccacggg cccggacttt tcaagttcct cgagtccgat
     6541 tgcagaggcc acatccaggt cggcaatccg ggtccgccgc agcgccgaca agtacccgcc
     6601 acaacccagt gcctcgccga tatcctgcgc caatacccgg atgtaggtcc ccttgctgca
     6661 ggccaccgtg atggacaaca tatttttctt taaatcaatt atttccagac tttttataag
     6721 aacgtttctc acgctccttt ccacctcgat tccggctcgg gcatacgcgt acaacggtcg
     6781 accgtccttc ttcagcgcgc tgtgcatcgg cggccgctgc aagatcggcc cggtgaagac
     6841 gcgcacgacc tcacgcaatt tatcgacgcc gaaatccggt acaccggctt cccgtatcgc
     6901 gccttcggca tccccggtcg agctgacaaa acccagtcgg atcaccgcct cataactttt
     6961 gtccgcatcg agcatcgagt aggaaaactt ggtggcctcg ccaaggcaga ttggcagcag
     7021 accggtggcc agcgggtcga gcgttccggt atgtcccgcc ttgatccgac cgaacaggcg
     7081 ccgtacacgt tgcaaagcgg cgttggaagt caggccggca ggcttgtcaa ggagcagaac
     7141 gccgtgaatc atgggacgag actgccgacg gtatataagg actttaccaa ggccgtcagt
     7201 cttggtcttt gccgggtttc accgatggcg gggaggccac agcttcatcg atcagcttgg
     7261 acaggcgcac gccgcgttcg accgattcat cgtggacaaa atggagttcg ggaacgatgc
     7321 gcagcttgag gccgccggca agctgggaac gcaggaagcc ggacgcgtgt tgcaggccct
     7381 gttgtgtctg cgaaacgacc tccgggccac ccagggtggt gaagaaaatc ttcgcgtgcc
     7441 ggtaatcgtt gctgacctcc acgccggtca gcgtaatcat gccaacccgc ggatccttga
     7501 gttcgctgcg gagcagcccc gccagatctc gctggatctg gtccgctata cgcccgagtc
     7561 tggaagggtc ctttgccatg ttctgcccaa tggatatacc ggaccgaccg ggttcggcgc
     7621 agcgaggcgc ggatgcgggg gaattcgcgc gccatgggtt cgcgagtgct tagtgttaaa
     7681 tgttgagtgc tgagttctga attaccctgc gcacccgtag cacctacaac tcaacactaa
     7741 aaactcaaca ctcaaaactg cgctcacagg gtacgcgcga tctcgacgac ttcgtagact
     7801 tcgagctgat caccgacctt gaggtcgtcg aaattcttga ccgaaagacc gcactcgaac
     7861 ccggctttca cctcgcgtac gtcgtccttg aaacgcttga gtgaatccag ttcaccggaa
     7921 aagatgacca cgttgtcccg caacacgcgc acctgtgatc ctcgacgcac cagaccttcg
     7981 agaacgtaac agccggccac tgtaccgacc ttggagatct tgaacacctg gcgtatctcc
     8041 accataccga tggtgctttc cttcttgtcc ggcgttagca tcccggacag cgcggctttg
     8101 atctcgtcaa ctgcctcgta aatcacgttg tagtagcgta attcgatgcc atgcgccgca
     8161 accagttttc ttgccattgt gtcggcccgc gtattgaagc cgatgatcac cgcattcgac
     8221 gccagagcca ggttgacgtc cgactcggta atcgcgccga cgcccgcatg gacgatattg
     8281 accttgacct cggcagtgga cagcttttcg agcgattgag acaacgcttc gtatgaacct
     8341 tgcacgtccg ccttgatgat caacgaaagc gcctttgcct gaccaacgga catctggtcg
     8401 aacataccct caagtttggc ggtctgttgt ttcgccagtt tgacctcgcg aaacttgccc
     8461 tgacggaaca acgcgatctc gcgtgccttg cgctcgtcgc ccagcaccac catctcctcg
     8521 ccggcgatcg gcacatcgga caagccctgg acctctaccg ggatggaggg gccggcctcc
     8581 ggcaccggat gtgcggcctc gtcgagcatt gcgcgaacac ggccgaatac ggcgcccaca
     8641 agtaccacat caccgcgctt gagcgttccc gactggacca gaatggtggc gaccggacca
     8701 cgacccttgt cgagccgtga ctcgatcacc acgccgtgcg ccggaacgtt cttaggcgcc
     8761 ctgagctcga gaacttcggc ctgaagcaga atgcgttcga gcaactcatc gatgcctttg
     8821 ccggattttg ccgagacatc ctggaacatc gtgtcgccac cccactcttc gggcacgacc
     8881 tgctctccgg cgagttgctg gcggattttt tccgggttgg cctccggctt gtcgaccttg
     8941 ttgacggcta ccaccagcgg caccttggca gccttcgcgt ggtgtattgc ctcgatggtc
     9001 tgcggcatca cgccatcgtc cgcagcgacc accagaacga ccagatcggt cactttcgca
     9061 ccacgggcac gcattgcggt gaaggcttcg tggccgggcg tgtcgagaaa agtgatcatg
     9121 ccgcgcggcg tgttcacatg ataggcgccg atgtgctggg tgatgccgcc ggcttcgccg
     9181 ctggccacgc gggtgcggcg gatatagtcc agcaaagatg tcttgccatg gtctacgtgg
     9241 cccatgacgg taacgaccgg tgcgcgtggt tccgccacta catcgtgctt ttccgcttcc
     9301 tcgctgagga aagactccgg atcatccagt ttggcgcgct tggcgatgtg gcccatttcg
     9361 tcgaccacga tcatcgctgt ctcctggtcg atcacctgat tgatcgttac catgctgccg
     9421 agcttcatca acactttgat gacttcggcg gccttcaccg acatcttctg agccagagcc
     9481 gcgacggtga tggtctccgg caccagcacc tcgcgtacga tcggttcagt cggcgcgttg
     9541 aacgactggt cggtggtgac cacctcgctc ttatgcttgg ggctgcggcc catgcgtgca
     9601 tgccaaccct ggccgccccg ggaatcgtcg ccgcgagtct tgagtcctcg gcgcctggcg
     9661 gattcatcct tccaggcggt ttcggatttc ttggcggttt tgggtttggc cggtttatct
     9721 cccggcttcg tcgcgggttt gtgcagcgtg ccttcggttg tcagcttctt ggcagccggt
     9781 ccttcagcgg gttgcggcgc cgccgcgacg accggtttct ccgactcagc cacggctgcc
     9841 ggcaccgagt ccttggccgt cttgcgcctg cgctgctgtt tctggaccgc ttcggcgctc
     9901 tgccgggcga tcagctccgc ctgtttacgc gcctcttcct cccgcaatgc cgtctgttcc
     9961 gcgtcgatcg cggcaggagg ggcggtttcg gcaacgggcg cgggcgcctc cgcagccgcg
    10021 gcatcacgct tgaccagaac gcgtttcttt ttgacttcga cctggattgt tcgcgccctg
    10081 ccggtactgt cggccttctt gatttccgtc gtctggcgcc gggtcagcgt gatcttgttc
    10141 ttggtgtcct tggccccatg aacgcgccgc aggtaatcca ggagctgggt cttatcctgc
    10201 tccgtgagca cggtatcttc ggcaagcgcc ttgcccactc ctgctgcctg cagctgctcg
    10261 aggagtaacg ccgaaggcag tcccagctcc ttggcaaatt gtgagacatt catctgcgcc
    10321 atatgtcgtt atccctggct tgccgcgaac cacggtgcgc gcgccgtcat gatcagttgc
    10381 ttggctcgct ccgcatcgat tcccgtcagt tccacaaggt catccaccgc cagatcggcc
    10441 aagtcctcct gggtggtcac gcctttcgcg gccagcacgc gtgccgtctc gttatccatg
    10501 ccctcgagcg tgagcaggtc gctggccacg ttttcgacat gctcctcgct ggcgatggcc
    10561 tcgacaagaa gcgcattgcg cgcgcggctc cgcagctcgt tgacggtatc ttcgtcgaaa
    10621 gactcgattt ccagcatctc gttgatcggc acataggcca cttcttcgag cgtcgtgaat
    10681 ccctcctgga tcagaatgcc ggcaacttcc tcgtccacgt cgaggcgttc catgaagagc
    10741 ttgcgtatcg ttgcggattc ctcctcgttc ttcttctggg acgcctcctc cgtcatgata
    10801 ttgagttccc agcccgacaa ctcgctcgcc agacgcacgt tttgtccgtt gcggccgatc
    10861 gcctgcgcaa gattctcctc gtcgacgacc acgtccatgc tgtgcgattc ttcatcaacg
    10921 gtgatcttgc tcacctcggc gggcgccagg gcattgatga cgaactgcgc aggatccggt
    10981 gaccacaggg tgatatccac gcgctcgccc gcgagttcgg cggtcacggc ctgtacccgt
    11041 gaaccacgca ttccgacgca ggtgccgatc ggatcaatgc gcggatcatt cgatttcacc
    11101 gcaattttgg cgcgggatcc cgggtcgcgc gccgccgcct tgatttccag taagccttcc
    11161 tcgatttccg gcacttccat ttcgaacagt ttgatcagga actccggcac cgtccgcgac
    11221 agaatcaact gcgggccacg attattgcgg tccaccttga gcagaaatgc gcgtacgcgg
    11281 tcgccgatcc gaaggttctc cttcgggatc atctgatcct tgggcaatgc cgcctcgatg
    11341 cgtccggact cgatgatcgc gttgccccgc tccatgcgtt tgatggcacc ggtgacgatg
    11401 aattccttac gctgcaggaa atcattgaga atctgctcgc gctccgcatc ccggatcttc
    11461 tgcaggatga cctgcttggc ggtttgcgcg ccgatgcgcc cgaactctac cggttcgagc
    11521 ggctcttcga cgaaatcatt gatctggaga ttcgggtccc gctcgtgggc atcggtcagg
    11581 gcgatctgct gcgccgggat ttcgtgatcg ttgtcggcaa cgatctgcca gcgccggaac
    11641 gacttgtact cgccagtctc gcggtcgatc tcaacgcgta catccacgtc gtctttgaaa
    11701 cgcttcttcg tagccgaagc caaggcaagc tcaagcgcgc cgaaaacaat gtccttctcg
    11761 acattctttt cgcgcgccag cgcatcaaca agcatcaaca gctcgcggct catttctgaa
    11821 ttctccagtc aaatattcgg gaccagacgg gctttattga tgtcggcaaa tgctatcgtt
    11881 atcagttcgc catcgacatc cagttgaaac ccgtcttccc ttacgttctg cagcactccg
    11941 gccactcgct tgcgaccgtt gatcgggatg cgcaatttca cttgaacgtg ttccccggaa
    12001 aatcgttcga agtctttcgc ccgcttcagt ggccggtcca gcccggggga cgaaatttcc
    12061 agccggtcat agtccacgtt ttcgacggcg aacaatcgcg tgaggtgatt gctgacggcg
    12121 gcacagtcgt caacattcac gccgccgacc ttgtcgatga aaatccgcat cagcctgcca
    12181 tgattggcaa gctcaacatc aacaagctcg tagccgagtc cgcccaccgt cttttcgagc
    12241 aatccatcca ggtccactag tatcgttttc acaaacaaaa aatgggcact cggcccacct
    12301 tgttttcaaa tcgggggaat tttagcaaaa ttcccgttaa aatcaaacgg caacaacacg
    12361 cgtcgttccg cgaaatgcct ccggatcgat accctgatgt cccaatatct aatgtgtgtt
    12421 gccagatcgc actttgcaag tacttggatg catacgttaa tgcaatgaca cgggtaagtt
    12481 tttttcgatc gaaatcggcg caggttcttt ttgacgcggc cgcaagtact gccagggcct
    12541 cgcggggcgg ggacctgctt gggctgccgc gtcacttgct cgcgcaggcg ggcaagtgag
    12601 tgtccgaaga tcctggcagt gaccgactct agttccgttc cgacatgacc tccgccaagg
    12661 cgtattgggc ggtgacctac tttagttgcg gcgtccgccg gaaacatacg gaacggtgac
    12721 ctactttggc tcccacgtaa cttgctcgct ggcgtgagca agtgagtgtc cgccgcaaca
    12781 aacccgcgag tgctcgtcga cgccaccttt gaggttctgg gaaacggggc cctaagtgcg
    12841 cacacgcgag gcgggcgtgt tgtcgtgcct caagtaggtc gctggcgggg ctcttaaaac
    12901 acaaagcccc tcgccggcta cccgggaggg gcttcgtgtt ttatgggcgc ctggcggtga
    12961 cctactttcg cgcgggcaat ccgcactagc atcggcgctg aggcgtttca ccgtcctgtt
    13021 cgggatggga aggcgtgggt ccacctcgct atggccgcca agcaaacggg tgaggggtaa
    13081 ggggtgaggc gtgaggggta aaaccccgcc gcggctccga agcccttacg ccccgtcatt
    13141 ctttgggcgt gtcgctaaca tctggaagaa gttttttggg ttcagatcag caacacaaga
    13201 cactgcagcc tttgaggggt gcaaaggttg ggtttgaagg tgttggtttt acccttcccc
    13261 cttcacgcct t
//
LOCUS       DASQOP010000274        14708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1940748, whole genome shotgun sequence.
ACCESSION   DASQOP010000274 DASQOP010000000
VERSION     DASQOP010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14708
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1940748"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VE030_10775"
     CDS             complement(<1..272)
                     /locus_tag="VE030_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23933.1"
                     /translation="MHRPIPPPLWAWQAITAFAGLFTLVSALADPVDLWRISVHSHQG
                     ELLKAVATLQALPEEHITQKCLSMGPESDAPGSDAPFLTNARLTLNA"
     gene            complement(385..1758)
                     /locus_tag="VE030_10780"
                     /pseudo
     CDS             complement(385..1758)
                     /locus_tag="VE030_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813082.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
     gene            complement(1908..2375)
                     /locus_tag="VE030_10785"
     CDS             complement(1908..2375)
                     /locus_tag="VE030_10785"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZF23934.1"
                     /translation="MESTLVGLKVPDFEIASTGNRTFKLSEELGSPLVLYFYPKDNTP
                     GCTTEGQEFRDLHGEFTRRGCAVYGVSRDSIKSHEGFKSKMSFPFELLSDPDEKACNL
                     FGVMKMKNMYGKKVRGVERSTFVIDPEGVVRREWRGLRVPGHAREVLEFVKSL"
     gene            complement(2641..3555)
                     /locus_tag="VE030_10790"
     CDS             complement(2641..3555)
                     /locus_tag="VE030_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZF23935.1"
                     /translation="MKLALKIDVDTYRGTRQGVPRLVKILTAHGAGATFLFSLGPDHT
                     GRAIKRVFRPGFFGKVRRTSVLKHYGLKTLLYGTLLPAPDIGRECADAMRNVRDAGFE
                     VGIHTWDHVLWQDEVAAADAQWTRGQMERACARFEEIFGEPARVHGAAGWQMNRYAYR
                     LTQRLGFNYCSDTRGSGPFIPIYQAEIIACPQLPTTLPTLDEMIGVDGVTGANVAERL
                     LELSRHPPVTGHVFTLHAELEGMKLAPVFEKLMEGWRAQGYELTSLHDYLESLATRNL
                     PRHEIAAGPVPGRAGALALQGREFLAGA"
     gene            complement(3559..4602)
                     /locus_tag="VE030_10795"
     CDS             complement(3559..4602)
                     /locus_tag="VE030_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HZF23936.1"
                     /translation="MKRILILGVNGFIGHHLSRRIIDTTDWEVYGMDMQTDRVTELLA
                     NKRFRFFEGDITINREWIEYHIRKCDTVLPLVAIATPATYVKEPLRVFELDFEANLPI
                     VRHAVKYGKRVIFPSTSEVYGMCRDGEFDPESSELIYGPINKPRWIYACAKQLMDRVI
                     AAYGQQEGLDYTLFRPFNWIGAGLDNIHTPKEGSSRVITQFFGHIVRGETIKLVDGGT
                     QKRAFTYIDDGIDALMKIIENRDGIASAKIYNVGNPKNNYSVRDLAKMMVDLALTYPE
                     YQAAAKLVKTVETTSDAYYGNGYQDVQNRVPKIENTRKDLGWQPKVDMQTALRNIFDA
                     YRSHVADARNLVN"
     gene            complement(4599..5543)
                     /locus_tag="VE030_10800"
     CDS             complement(4599..5543)
                     /locus_tag="VE030_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase"
                     /protein_id="HZF23937.1"
                     /translation="MARAVIFAYHNVGSRCLQATLARGLEVPLVVTHRDNPAENIWFE
                     SVAGIAQENDIPTIAPDDPNLPETVERVAAARPDFIFSYYYRHMLGAALLATAARGAL
                     NIHGSLLPKYRGRVPINWAVINGETETGASLHYMVEKPDAGDIVDQHPVPILPNDTAL
                     DVFLKVTVAAELVLHRSLPALLAGTAPRHVQDLSKGSYFGGRKPEDGRIDWRQNVEAV
                     HNLVRGVAPPYPGAFTTVAGQTIFVFRAARQTGLRRRFPQPTLFVEDGRCLAQCGDGG
                     VLRLVHLQANGVTLDAPAFVRLFGPAPLPLLSGSLIGT"
     gene            complement(5549..6559)
                     /locus_tag="VE030_10805"
     CDS             complement(5549..6559)
                     /locus_tag="VE030_10805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZF23938.1"
                     /translation="MENPALSVVIPVYNEEAGLPALFARLYPALDSLGIRYEIIFVND
                     GSKDRSAAALRQQFERRPDVTRVILFNANYGQHLAIMAGFQRTRGEVIVTLDADLQNP
                     PEEIGKLLAKMDEGYDYVGGVRAERQDTWFRRNASQLMNRMRERTTHIHMTDQGCMLR
                     AYSRQIVNAINQCEELNTFIPALAYTFASNPTEIEVLHEERAAGTTKYSLYNLMRLNF
                     DLVTGFSLAPLQAFSMAGFAIAVCASGFVVFHSVHRLLLGEAAEGVFSSVLDGIQFFL
                     FGVMLFGIGLLGEYIGRIYQEVLRRPRFLISAVLEDLSDEQSAGATAVQSAEARRQSM
                     LR"
     gene            complement(6582..7775)
                     /locus_tag="VE030_10810"
     CDS             complement(6582..7775)
                     /locus_tag="VE030_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003266235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HZF23939.1"
                     /translation="MIANAPLHTVNSGPSNSGARSYLPFARPTIDEETIAGVAEVLRS
                     GWITSGPNVLKFEQALSEYHGGRPTRVVASATAALELALQVAGVGPRAEVITSAMSFF
                     AAPNMIVKVGAKPVFVDCDLITRNIDFDQVEAAITARTTAIMPTHFAGLPVDIERLYA
                     IAQKYGLRVIEDAALAIGSRWQGRRIGAFGDLVVFSFHPNKNMTTIEGGALLMGTEAE
                     VKLFEKLRFHGIVRLADGTRDVDVASGKFNLPDVNARIGLAQLARLDEFNAARRMLAA
                     RYFANLRTDPQCLLPAHAHAGEEEGHSWNMFCVLLPLEQLDITRKQFIDAMHARGIGI
                     GISYEALHLTTLCRGFGHREGDFPNAERIGRETVTLPLFPAMTAGDVDRVCEAVADVL
                     RNAKR"
     gene            complement(7772..8143)
                     /locus_tag="VE030_10815"
     CDS             complement(7772..8143)
                     /locus_tag="VE030_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006482863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HZF23940.1"
                     /translation="MNLVSFSLVLAGVLLNAAAQLLLKAGTNRIGTFAFAPESILPIG
                     LRIATEPFVLGGLGCYVISVLIWIMALSRVEVSIAYPMLSIGYVVNALAAWYLFGEAV
                     TAQRMVGIGTIIVGVFLVARS"
     gene            complement(8140..9792)
                     /locus_tag="VE030_10820"
     CDS             complement(8140..9792)
                     /locus_tag="VE030_10820"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZF23941.1"
                     /translation="MSDPNTFRRAVFAAALSLLAVVWFGVIEYRKLVKPDEGRYAEVP
                     REMVATGDWLTPRLNGIKYFEKPPLQYWATAAAYSTFGQHHWTARLWSALAGFLGLFV
                     IWFTGRQLFGEPAGTLAAMVAGSSLLYAVIAHINTLDMGLTFFMGSSLCAFLLAQREG
                     TNAKEHRLWMALSWAATAAAILSKGLVALILPGLAIFVYSLLRRDWRIWLRLDALLGM
                     LLLAAIAAPWFVWVSIVNPEFPGFFFVHEHFARFLTTVHRRVEPWWFFIPILVLGLMP
                     WTATAFAAAWQSWRAGAQTSGFDPGRFLLVWFAVVFVFFSASGSKLPSYILPMFPAAA
                     LFIGRYLTQAGPRAVAWQLVPMALLGLALLAVTPFVNFGRKSEVPAELYDHFAVWLQV
                     AGAISVAGSVAAIAFLLRKRLLPAIAAAAACALLSVQVAGLGHDSLNYVTSAYHLAQR
                     INPILRPGVPFYSVRTYEQTLPFYIGRTLTLVEFADELDFGLKQEPQLWVPTLAEFEK
                     RWKEHDDAFAVMSPDTYSELEGHGLPMQIVASDPRRIVVRKK"
     gene            complement(10037..11098)
                     /gene="mnmH"
                     /locus_tag="VE030_10825"
     CDS             complement(10037..11098)
                     /gene="mnmH"
                     /locus_tag="VE030_10825"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010398352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HZF23942.1"
                     /translation="MNQDTVTLAQLSEFDEVVDVRSPAEFALDHIPGAINCPVLDDDE
                     RARVGTLYVQVSPFTARKVGAALVARNIARHVESSFAGKPKTWRPLIYCWRGGQRSLA
                     MGHVMRQIGWDAKRLAGGYKAYRHAVVAGIETLAGKFDYRVVCGLTGTGKSILLQHLG
                     RLGAQTLDLEQLAAHRGSVLGELPGEPQPSQKMLESRLWQKLTSLDPRRPVFVESESR
                     KVGNLRVPEPLIARMWQSECVWLETVAPVRVALLLRDYRHFTEDIQALCARLECLVRL
                     HGQEKINGWKNKVASAQWPEFVTQILQEHYDPAYVKSIARNYPKLGRATYVRMTDGSD
                     TDFERAACSLLEPALAETT"
     gene            complement(11173..11790)
                     /locus_tag="VE030_10830"
     CDS             complement(11173..11790)
                     /locus_tag="VE030_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HZF23943.1"
                     /translation="MWLRYLLLLVLTAVAPAHAARTILVFGDSLSAGYGLATGSGWAS
                     LLGHRLKRDKLDYTVVNASISGETTLGGRNRIAAALAEHQPAVVVVQLGGNDGLRGNS
                     IEETRRNLIAIVEASRNAGAKVLLVGMRIPPNYGQVYTRRFEALFAEVARQQNASLVP
                     FMLQGFADKREWFQSDGIHPTAEAQPRILDNIYRRLRALLTTQRA"
     gene            11789..12484
                     /locus_tag="VE030_10835"
     CDS             11789..12484
                     /locus_tag="VE030_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZF23944.1"
                     /translation="MSQEPFPPIVKAVGLTKQVSTGATTLTILQDISLEIAPGEACAI
                     LGASGSGKTTLLALLAGLDVPTAGEVHINGANLFALDEDGRARLRRNQVGFVFQSFQL
                     LPALTALENVMLPLELAGNDGAREKAAGWLARVGLAERLEHYPRQLSGGEQQRVAVAR
                     AFAAEPRLLFADEPTGNLDSATGEEIISLMFALNRESRTTLLLVTHDEAIARRCPRQL
                     KLAAGRLTSDERR"
     gene            12471..>14708
                     /locus_tag="VE030_10840"
     CDS             12471..>14708
                     /locus_tag="VE030_10840"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZF23945.1"
                     /translation="MSAADRLNAGAVLRLASRMLGRDWRAGEQRVLAVALIVAVASLT
                     TVAFFADRVGRTLTREANQLLGADLVVVSDAPIAESFRGEAFLLGLAVTEAVRFPSMT
                     QAKDRALLTEVKAVAAGYPLRGKLRIRERAAGPDFEPARIPAPGSVWADDRLLRRLDV
                     SIGERIALGKREFTVAAIVSEEPESSAGFLNLGPRLMLNMDDLASTALVQVGSRVSYR
                     LFVAGDTQRVAVFRDYAAQTIRPGQRVEDIREARPEINSALTRAERFLGLSSLLTVIL
                     AGVAVALAARRYLQRHLDACAIMRCLGASQTLILMLHVLQFVIIGLIASAIGCAIGFL
                     CQYLLAALLAPIVSVTLPAPGWLPVLQGLIAGFVLLLGFALPPLIALKKVPTLRVLRR
                     DLGAPDSFGLSAYLLGGAAMSGLILWQAQDPKLGMYVLGGVVGTMIASAVLTLLVLRL
                     LSSFSRGARLNWRFGLANLRRRTMGTVIQVIALGLGMMALILLTLVRSDLLESWKRSL
                     PPDAPNRFLVNIQPDQVGEIADFFAVRHLHRPALFPMIRGRLVEVNGKPVSSKDYVED
                     QARRLIDREFNLSWAEKMQPGNVIVAGRWFGASDAGKPLLSVEEGIAKTLSLKLGDDL
                     TYDIGGSRLTAKISSLRTVDWDSFRVNFFVIAPPGVLERYPGSFVTSLYLPASRSVVM
                     DDIVKRFPNLLVIDVAAILAQVQRMMDQVVRAIEFVFLFSLAAGLLVLFAAVSSTHDE
                     RLFDAA"
BASE COUNT         2644 a         4500 c         4728 g         2836 t
ORIGIN      
        1 gcgttcagcg tgaggcgggc attggtcagg aaaggggcat cggaacccgg tgcatcgctc
       61 tccgggccca tggacagaca tttctgggtg atgtgttctt ccgggagcgc ctgcagggtg
      121 gccaccgctt tgagcagctc accttggtgc gagtggacgc tgatgcgcca cagatctacc
      181 gggtccgcga gcgcggaaac cagggtgaat aacccagcga aggccgttat cgcctgccac
      241 gcccaaagtg gtggtgggat cggtctatgc actcgctttt gatctatatc ttgtagggtt
      301 tcagcgcata tgcaccatat atagtgtccg ggagtttagt cggttagcgg atgccgtgca
      361 atgcctgcgg ctacgcgcag ggcgtcaaag aatttcggca gcgtggtcgg ccagccgcga
      421 tctctcgccg cgcatgaggg tcacgtggcc gctatgggcc caacccttga agcgatcgac
      481 gacgtaggtc agtccggaac tgccctcggt gagatagggc gtgtcgatct gggaaatgtt
      541 tccgagacat acgaccttgg tgccgggccc ggcgcgggtg atcagcgtct tcatctgctt
      601 gggggtaagg ttctgtgcct catcgatgat caggaactta ttgatgaagg tgcgcccacg
      661 catgaaattg agcgatttga ccttgatgcg ggagcggatc aggtcgcggg tggccgcgcg
      721 cccccattcg cccgcctcgt cgtcggtctt gttgagcacg tcgaggttgt cctccaacgc
      781 tcccatccac ggcgtcattt tttcctcctc ggtgccgggc aggaagccga tatcctcgcc
      841 taccgggacg gtgacgcgag tcatgatgat ttccgagtag accttgtcct cgagcgtctg
      901 catcaggccg gcggcgagcg tcagcagtgt cttgccggtg ccggcctggc cgagcagggt
      961 cacgaaatcg acctccggat tcatcagcag gttgagcgcg aagttctgct cgcgattgcg
     1021 cgcggtgatg ccccacacgt tgttcttctg gtgggcgtag tccctgatgg tctgcaggat
     1081 cgcggtcttg ccgctctgct cttttacctg cgcgaagaac ggtttgtcgg tttcatggta
     1141 gatgaattcg ttcaccatca agcttgcgca cagcggcccg cgcacgcgat agaaggtgcg
     1201 gccttcctgc tgccaggact ccatttcctt gccgtgctgg tcccagaagt cggcgggcaa
     1261 ctcacgcacg ccggtgtaga gcaggtcggt atcctccagc accttgtcgt tgaagtagtc
     1321 ttctgccgcc aggcccagcg cacgcgcctt gatgcgcatg ttgatgtcct tgctgaccag
     1381 gatcacctcg cgcttgtcgt gtccctcctg gaggtgcttg accacgccga tgatctggtt
     1441 gtcggccttg ccggcggcca accgcgccgg caactcgccg ttgatcgcct cggtctgcag
     1501 aaacaggcgt ccggtcgcct ccttggcacc ggcgttttcc aacggaatgc cggcggaaat
     1561 atcctgcacc gccttggaga cgatctcgtc gagaaagcgg cttgcctggc gggcgttgcg
     1621 cgcaacctcg gacatgcctt tcttgttgga gtccagttcc tccagcgtgg ccatcggcag
     1681 gtacacgtcg tgttcctgga agcggaacag gctggtggga tcgtgcatca gcacgttggt
     1741 gtcgaggacg aacagcttgg tgaggggctg cagcttggtg actaacgtgg gaccgggttt
     1801 gcgctggatc atcggattgc cccggcaagg cgagagagga aaagaaagcg aaattgaatg
     1861 gaagacctac tccatcgggg gatcatccgc gctcctgtgg ttgacggtta aagggatttt
     1921 acgaattcca gaacctcccg ggcgtggccg ggcaccctca gcccgcgcca ttccctgcga
     1981 accacgccct ccggatcgat gacaaaagtg ctgcgctcca cgccccgcac ctttttgccg
     2041 tacatatttt tcattttcat gacgccgaac aggttgcagg ccttttcgtc ggggtcggac
     2101 aacagttcga aggggaaact catcttggac ttgaagccct cgtgcgactt gatgctgtcg
     2161 cgcgagacgc cgtagacggc gcagccgcgg cgggtgaact cgccgtgcag gtcgcgaaac
     2221 tcctgacctt cggtggtgca gccgggggtg ttgtccttgg gatagaagta gaggaccagc
     2281 gggcttccca gctcttccga gagcttgaac gtccggttcc cggtcgaggc aatctcgaaa
     2341 tccggaacct tgagacccac caaggttgat tccattcctg cggactatgt ttaccgaaac
     2401 cttggccgaa tgcaataccc aattagagcc tcaccgcaga ggcgcaaagg cgcagaggaa
     2461 acgcaaagga aagtaaagca acttgctgtg aaaaatgcaa gattatgaag tgaatcgtcg
     2521 cgacatcacg aatggtcgat cgggtggtgg caaatcggaa tcctcccgct ttttgacacg
     2581 cttccttggg ttttctctgc gtttcctttg cgcctttgcg cctctgcggt taagccttag
     2641 tcaggcgccg gcgaggaact cccggccctg cagcgccaac gccccggcac ggccgggaac
     2701 cggtccggcg gcaatttcat ggcgcggcag attccgggtc gccagcgatt ccaggtagtc
     2761 gtgcagcgaa gtcagttcgt agccctgggc gcgccacccc tccatcagct tctcgaaaac
     2821 cggggcaagt ttcattccct cgagttcggc gtgtagcgta aaaacgtggc cggtgacagg
     2881 cggatgccgt gacaattcga gcagccgttc agcgacgtta gcgccggtca cgccatccac
     2941 tccgatcatc tcgtccagcg tgggcagggt ggttggcagc tgcgggcagg cgatgatttc
     3001 cgcctgatag atcgggataa aaggtccgct cccccgggta tcggaacagt agttgaagcc
     3061 caggcgctgg gtcagtcgat aggcgtagcg gttcatctgc cagcccgcgg cgccgtgcac
     3121 ccgggccggc tcgccaaaaa tctcctcaaa gcgggcacag gcccgttcca tctgccctcg
     3181 cgtccactgc gcgtcggcag cagcgacctc gtcctgccag agcacatggt cccaggtatg
     3241 gatcccaacc tcgaacccgg cgtcgcggac attgcgcatg gcgtcggcgc actcccggcc
     3301 gatatccgga gcgggaagaa gggtgccgta gagcagcgtc ttgagtccgt agtgcttgag
     3361 caccgaggtt cggcgcactt tgccaaagaa tcctggacgg aacacccgct tgatcgcgcg
     3421 gcccgtgtga tcagggccaa ggctgaaaag aaaggtagcc ccggcgccgt gcgccgtgag
     3481 gattttcacc agccttggca cgccttggcg tgtgccgcgg taggtatcga cgtctatctt
     3541 gagtgccagt ttcatggatc aattgaccag attgcgggca tcggccacgt ggctgcgata
     3601 agcatcgaag atattcctta gcgcagtctg catatcgact ttcggttgcc agccaagatc
     3661 cttgcgggtg ttttcgatct tcggaacccg gttctgcacg tcctggtaac cgttgccgta
     3721 ataggcgtcg gaggtggttt cgaccgtttt taccagcttc gccgccgcct ggtactcggg
     3781 ataggtgagg gcgagatcga ccatcatctt cgccagatcg cgaaccgaat agttgttttt
     3841 cgggttgccg acattgtaga ttttggcgct ggcgatgccg tccctgtttt cgatgatctt
     3901 catcagcgca tcgatgccgt cgtcgatata ggtgaaggcg cgtttctggg tgccgccgtc
     3961 caccagcttg atggtctcac cgcgcacgat gtggccgaag aattgcgtga tcacccgcga
     4021 gctgccttcc ttcggcgtgt ggatgttgtc cagcccggcg ccgatccagt tgaagggacg
     4081 gaacaaagtg tagtcgagcc cttcctgctg gccataggcg gcgatgacgc ggtccatcaa
     4141 ctgcttggcg caggcgtaga tccagcgcgg cttgttgatc ggcccgtaga tcaactcgga
     4201 actttccgga tcaaattcgc catcccggca cattccatag acttcggacg tggaaggaaa
     4261 gatcacccgt ttgccgtact ttaccgcgtg gcgcacgatt ggcagattgg cctcgaaatc
     4321 cagttcgaac acccgcagcg gctccttgac gtacgtggcc ggcgttgcga ttgccactaa
     4381 cggcagtacc gtgtcgcact tgcgtatgtg atactcgatc cactccctgt tgatggtgat
     4441 gtcgccttcg aagaagcgga agcgcttgtt cgcgagcagc tcggtgaccc ggtcggtctg
     4501 catgtccatt ccgtacacct cccagtcggt ggtgtcgatg attcgcctgg acaaatggtg
     4561 gccgatgaag ccgttcacgc cgaggatcag aattcttttc atgttccaat cagactccct
     4621 gaaagtaatg gcagcggtgc gggaccgaac agccggacga aagccggggc gtccagcgtt
     4681 accccgttcg cttgcaggtg caccagtcgc aacacgccgc cgtcgccgca ctgcgccagg
     4741 cagcgtccgt cttcgacaaa cagcgtcggc tgtgggaagc gcctgcgcag tccggtctgc
     4801 ctcgcggccc tgaatacgaa aatggtttgg cccgcgacgg tcgtgaacgc gccggggtag
     4861 ggcggcgcga cgccgcgcac taggttgtgc accgcttcga cgttttgacg ccagtcgatt
     4921 cgcccgtcct ccggtttgcg gccgccgaag taagaaccct tcgacaggtc ctgcacatgg
     4981 cggggggcgg tgccggcgag cagcgccggc aggctccggt gcagcacgag ctcggccgca
     5041 accgtcactt tgagaaacac gtcgagtgcg gtgtcgttgg gaaggatagg caccggatgc
     5101 tgatcgacga tgtccccggc gtcgggtttt tcgaccatgt aatgcaagct cgccccggtt
     5161 tcggtttcgc cgttgatgac cgcccagttg atcggtactc ggccgcgata tttcggcagc
     5221 agcgagccgt ggatgttgag tgcgccccgt gccgcggtgg cgagcagtgc cgcgccgagc
     5281 atgtggcggt agtaatagga gaaaatgaag tccggccggg cagcggcgac gcgttccacc
     5341 gtttcaggca ggttcgggtc atcgggggcg atcgtcggga tgtcgttttc ctgcgcgatt
     5401 ccagcgacgc tttcgaacca gatgttctct gccgggttgt cccgatgtgt caccaccagc
     5461 gggacttcga gcccgcgtgc gagcgtggcc tgcaggcagc gtgagcccac attgtggtag
     5521 gcgaagatga ctgcgcgagc catggaagtc aacgcagcat cgactgcctg cgcgcttccg
     5581 cagattggac tgccgttgcg ccggcagatt gttcgtcgga caggtcttcg agcaccgcgc
     5641 tgatcaggaa gcgcggacgg cgcagcacct cctggtatat gcggccgatg tactccccga
     5701 gcagcccgat tccaaacagc atgacgccga acaagaagaa ttggatgccg tcgaggaccg
     5761 agctgaacac cccctccgcc gcctctccga gcaacaggcg atgcacggag tgaaacacca
     5821 cgaacccgct cgcgcagacg gcgatcgcga aaccggccat ggagaacgcc tgcagcggcg
     5881 caagcgaaaa gccggtaacc agatcaaaat tcaggcgcat gaggttgtac agcgagtact
     5941 tggtcgtccc cgcggccctt tcctcgtgca agacctcgat ttcggtcgga ttgctggcga
     6001 aggtgtaggc gagcgcaggt atgaaggtat tcagctcctc gcactgattg atcgcgttaa
     6061 cgatctggcg ggagtaggcg cgaagcatgc aaccctggtc ggtcatgtgg atgtgggtgg
     6121 tgcgctcccg catccggttc atcaattgcg aagcgttgcg ccgaaaccat gtgtcctggc
     6181 gctcggcgcg cactccgccg acgtagtcgt aaccctcatc catcttggcc agcagcttgc
     6241 cgatttcctc gggcgggttc tgcagatcgg catccagcgt gacgatcacc tcgccgcgcg
     6301 tgcgttggaa accggccatg atcgccaggt gctggccgta gttggcgttg aacaggatca
     6361 cgcgggtcac gtccgggcgg cgttcgaact gctggcgcag ggcggcggcg gagcgatcct
     6421 tgctgccgtc gttgacgaaa ataatttcgt agcggatgcc gagcgaatcg agcgcgggat
     6481 acagccgtgc aaaaagagcc ggcagccccg cctcttcgtt atagaccgga atcaccaccg
     6541 aaagcgccgg attttccatt ttattccgtg cgttcgctcg gttagcgttt tgcgttgcgc
     6601 aggacatcgg caaccgcttc gcagactcga tccacatcgc cggcggtcat cgccgggaac
     6661 aacggtagcg tcaccgtttc gcgcccgata cgttcggcgt tgggaaagtc gccctcgcga
     6721 tgcccgaagc cgcggcacag cgtggtcaga tgcagagcct cgtaggaaat accgatgccg
     6781 attccgcgcg cgtgcatggc gtcgatgaat tgtttgcgcg tgatgtcgag ctgctcaagc
     6841 ggcagcagca cgcagaacat gttccagctg tggccttctt cctcgccggc gtgcgcatgc
     6901 gccggcagaa ggcattgcgg atcggtgcgc agatttgcga aatagcgggc ggccagcatg
     6961 cgccgtgccg cattgaattc gtcgagccgg gcgagctgtg ccagaccgat gcgggcgttg
     7021 acgtccggca ggttgaactt cccgctggcg acgtccacgt cgcgggtgcc gtccgccagg
     7081 cgcacgatgc cgtgaaagcg cagcttctcg aacaacttta cctcggcttc agtgcccatc
     7141 aacagcgcgc cgccctcgat ggtggtcatg ttcttgttcg ggtggaagct gaacaccacc
     7201 aagtccccga atgcgccgat gcgccgaccc tgccagcgcg agccgatcgc cagtgcggcg
     7261 tcttcgatca cccgcagccc gtacttttgg gcgatggcgt acaggcgctc gatgtccacc
     7321 ggcagtccgg cgaaatgggt cggcatgatc gccgtggtgc gcgctgtgat tgcagcttcc
     7381 acctggtcga aatcgatgtt gcgcgtgatc agatcgcagt ccacgaacac cggctttgca
     7441 ccgactttta cgatcatgtt gggcgccgcg aaaaaactca ttgccgacgt gatcacctca
     7501 gcgcgggggc cgacaccggc aacctgcagg gccagttcga gggctgcggt ggcggaagcc
     7561 actacccgcg ttggccgccc cccgtgatat tcagaaaggg cctgctcgaa cttgagcacg
     7621 ttcggcccgc tggtaatcca gccggagcgc aaaacctccg ccacgcccgc gatggtttcc
     7681 tcgtctatgg tcggccgcgc gaaaggcagg taagaccgcg ccccgctatt tgaagggccg
     7741 gaattcacgg tgtggagagg cgcgttggcg atcatgagcg tgcgaccagg aatactccga
     7801 cgatgatggt gccgatgccg accatccgct gagcggtcac tgcctccccg aacaggtacc
     7861 aggcggccag cgcgttgacc acgtagccga tggacaacat cgggtacgca atactcactt
     7921 cgacgcggga caaggccatg atccagatca aaacactgat gacataacag ccgagcccgc
     7981 cgagcacgaa cggttcggtg gcaatgcgca ggccgatcgg caggatgctt tccggagcga
     8041 aggcgaaggt gccgatgcga tttgtgccgg ccttgagcag aagctgcgct gcggcgttca
     8101 gcagcacccc ggcgagcacg agggaaaagc tgaccaggtt cattttttcc tcaccacaat
     8161 tcgacgcgga tcggaagcga cgatctgcat gggcaggccg tggccttcga gctcgctgta
     8221 ggtgtcggga gacatgaccg cgaaggcgtc gtcatgctcc ttccagcgct tctcgaactc
     8281 ggccagcgtc ggtacccaga gctggggctc ctgcttcagg ccgaaatcga gctcgtcagc
     8341 aaactcgacc agagtcagcg tccgccctat gtagaacggc aaggtctgct cgtaggttcg
     8401 cacgctgtag aagggcacgc cgggcctgag gatgggattg atcctctgcg ccaaatgata
     8461 cgccgaagtc acgtaattca ggctgtcgtg tccgaggccg gcgacttgca ccgacagcaa
     8521 tgcgcacgct gcggcagcgg cgatcgcggg aagcaggcgt ttgcgcagga ggaacgctat
     8581 ggcggccacg ctgcctgcga cgctgatcgc cccggccacc tgcagccaca ccgcgaagtg
     8641 gtcgtagagc tccgccggca cctcgctctt gcggccgaag ttcacgaacg gcgtgaccgc
     8701 cagcagtgcc agcccgagca gcgccatcgg tacgagctgc caggcgacgg cgcgcgggcc
     8761 ggcctgtgtc aggtagcggc cgatgaacag ggctgctgcc gggaacattg gcaggatgta
     8821 cgagggcagc ttcgatcccg acgcgctgaa gaacacgaac accacggcaa accagacgag
     8881 caggaaacgc ccggggtcga atccgctggt ctgcgcgccc gcgcgccagc tctgccatgc
     8941 ggcggcaaat gcggttgcgg tccagggcat cagcccgagc acaagaatgg gaatgaagaa
     9001 ccaccatggt tccacccgcc tatgaacggt ggtcaggaaa cgcgcgaaat gctcgtggac
     9061 gaagaagaag ccgggaaact cggggttgac gatcgatacc cagacgaacc agggcgcggc
     9121 gatcgccgcc aacagcaaca tccccagcag tgcgtcgagc cgcagccata tgcgccagtc
     9181 gcgacgcagc agcgaataaa cgaaaatcgc cagccccggc aggatcagcg caaccaggcc
     9241 tttgctcagg atcgccgccg cggtcgcggc ccatgacaac gccatccaca gcctgtgctc
     9301 cttcgcgttc gtgccttcgc gttgcgccag caggaacgcg cacagcgacg agcccatgaa
     9361 aaacgtgagc cccatgtcga gcgtgttgat gtgggcgatg acggcgtaga gcagactgct
     9421 gcccgctacc atcgcggcaa gcgtgccagc cggttcgcca aacagctgcc ggccggtgaa
     9481 ccagatcacg aacaggccga gaaacccggc aagcgcgctc cacaggcgcg cggtccagtg
     9541 atgttgcccg aatgttgagt acgcggcggc tgtggcccag tattgcagtg ggggcttttc
     9601 gaaatacttg attccgttga gacgaggcgt gagccagtcg ccggtagcga ccatctcgcg
     9661 aggaacctcg gcgtatcggc cttcgtctgg cttgaccagc tttcggtact cgatgactcc
     9721 gaaccagacg acggcgagga gcgacagcgc agcggcgaaa acggcgcggc ggaacgtgtt
     9781 gggatcagac atcggttcca ttgtgccggg catcgggagg cccgacaagg agtagccgcg
     9841 aaattacggc aaacttgtca ccgtaaagac gcaaagaact caaagaaaag cagaaaaaat
     9901 agccgaggaa aacctgcaat gtgcagtacc tgctttcgtc gcgagtatcg gctatccaga
     9961 tatagaacga catgaaccgt tttttttatt gcctgggttt cctttgcgct ctttgcgtct
    10021 ttgcggtgaa aattccttag gtcgtttccg caagggcggg ttcgagcagc gagcaggcgg
    10081 cgcgctcgaa gtccgtgtcg ctgccgtcgg tcatccgcac gtaagtggcg cggccgagct
    10141 tgggatagtt gcgggcaatg gacttgacgt acgcgggatc gtagtgttcc tgaagaatct
    10201 gcgtcacgaa ttccggccac tgggcgctgg cgaccttgtt cttccagccg ttgatcttct
    10261 cctgtccatg cagccgcacc aggcactcga gtctggcgca aagcgcctgt atgtcttcgg
    10321 tgaagtgtcg gtagtcgcgc aacagcagcg ccacgcgcac cggcgcgacc gtctccagcc
    10381 agacgcattc gctctgccac atgcgcgcaa tcagcggctc cggcaccctg aggttgccga
    10441 ccttgcggct ttccgactcg acgaacaccg gccggcgcgg atccaggctc gtgagtttct
    10501 gccacagccg gctttccagc attttctgcg agggctgcgg ctcgccgggc agttcgccca
    10561 gcacggaacc gcggtgcgcg gcaagctgct cgagatccag ggtctgcgca ccaagccgcc
    10621 cgaggtgctg cagcaaaatg ctcttgccgg tgccggtgag gccgcagact acccgataat
    10681 cgaattttcc ggccagggtt tcgattccgg cgaccaccgc gtggcggtag gccttgtatc
    10741 cgccggccag ccgcttcgcg tcccagccga tctggcgcat cacgtggccc atggccaggc
    10801 ttctttgccc gccgcgccag cagtagatca gcggccgcca cgtcttcggc ttgccggcga
    10861 aactcgattc gacgtgccgc gcgatgttgc gcgcgaccag agccgcgccg actttcctgg
    10921 cggtgaaagg cgaaacctga acgtagagcg tgccgacgcg tgcgcgctcg tcgtcgtcga
    10981 gcaccggaca attgatcgcc cccgggatat ggtcgagagc gaattcggcg ggagagcgta
    11041 cgtcgacgac ctcgtcgaat tcggacagct gtgctaacgt gaccgtgtcc tggttcattc
    11101 gacgcagcgc gctggcctgg ttggaaacgt catgagttta cacgcgcggg caccggcact
    11161 atcctccgcg cgtcaagccc gttgcgtcgt aagcaaggca cgcaggcgcc ggtagatgtt
    11221 gtcgaggatg cggggttggg cctcggcggt gggatggatg ccgtcagact ggaaccattc
    11281 gcgcttgtcg gcgaagccct gcagcatgaa cggaaccagc gatgcgttct gctgtctagc
    11341 cacctcggcg aacagcgctt cgaagcgccg cgtatagacc tggccgtagt tgggcggaat
    11401 gcgcattccc accaacagca ctttggcgcc ggcattgcgt gaggcctcta cgatggcgat
    11461 caggttgcgg cgcgtttctt cgatagagtt tccgcgcagg ccgtcgttgc ccccgagctg
    11521 gacgacgacc accgccggct ggtgctccgc cagcgcagcc gcgatgcgat tgcggccgcc
    11581 gagcgtggtt tcgccgctga tgctggcgtt gacgaccgta tagtcgagct tgtcgcgctt
    11641 gagccgatgt cccagcaagc tcgcccagcc gctgccggtc gcaagcccgt agcccgcgga
    11701 caggctgtcg ccgaacacca gtatcgtccg ggcggcatgc gcgggcgcaa ccgcagtcaa
    11761 gaccaacagc agaagatatc gcagccacat gtctcaggaa cctttcccgc caattgtgaa
    11821 ggcggtcgga ttgacgaagc aagtctccac cggcgccacc acgctcacca tcctccagga
    11881 catatcgctg gaaatcgcgc ccggggaagc ctgcgccatc ctcggcgcgt ccggctcggg
    11941 caagactaca ctgcttgcgc tgcttgcggg gctggacgta cccaccgccg gggaggtgca
    12001 catcaacggc gccaacctgt tcgcgcttga cgaggacggc cgcgcgcgcc tgcgccgcaa
    12061 ccaggtcggg ttcgtgttcc agtcgttcca gcttcttccg gcgctgaccg cgctggaaaa
    12121 cgtcatgctg ccactcgagc ttgccggcaa cgacggcgcg cgcgagaagg ctgcgggctg
    12181 gctggcgcgt gtgggcctgg ccgagaggct cgaacattat ccgcggcaac tgtccggcgg
    12241 ggagcagcag cgcgtggcgg tagcgcgcgc cttcgccgcc gaaccgcggt tgctgttcgc
    12301 cgacgagccc accggcaacc tcgactccgc caccggcgag gagatcatct cgctcatgtt
    12361 cgcgctcaac cgggaaagcc gcaccacgtt gctgctggtg acccacgacg aggcgattgc
    12421 ccggcgctgt ccgcgccagc tcaagcttgc cgccggcagg ctgaccagcg atgagcgccg
    12481 ctgatcgctt gaacgccggt gcggtgctgc gactggcgtc gcgcatgctc ggtcgcgact
    12541 ggcgtgcggg cgagcagcgc gtgctggcag tggcgctgat cgtagcggtg gcaagtctga
    12601 ccacggtggc cttctttgcc gaccgcgtgg ggcgcactct cacgcgcgaa gccaaccagt
    12661 tgctcggcgc cgacctggtc gtggtctccg acgcaccgat cgcggaatcg ttccgcggcg
    12721 aggcgttcct cctcgggctt gccgtcacag aagccgtgcg ctttcccagc atgacgcagg
    12781 cgaaagatcg tgcgctgctg accgaagtga aggcggtggc ggcgggatac ccgctgcggg
    12841 gcaaactgcg catccgcgag agggcagccg ggccggactt cgagcccgcg cgcattccgg
    12901 cgccgggcag cgtctgggcg gacgatcgcc tgttgcgccg gctcgatgta agtatcggcg
    12961 agcgcattgc tctcggaaag cgtgagttca cggtggcggc tattgtgagc gaggagccgg
    13021 aatcgagcgc cggatttctc aatcttggcc cgcggttaat gctcaacatg gacgatctcg
    13081 cctccactgc cctggtgcag gtcggcagcc gggtctccta ccgcctgttc gtagcgggcg
    13141 acacgcaacg agtggcggtg tttcgcgatt acgccgcaca gacgatccgg ccgggccagc
    13201 gggtcgagga catccgcgaa gcgcgtccgg aaatcaactc ggcgctgacc cgtgccgaac
    13261 gatttctcgg cctgtcctcg ctgctgacgg tcattctcgc cggggttgcc gtggcgctgg
    13321 cggcgcgccg ctatctgcag cggcatctgg atgcctgcgc aatcatgcgc tgcctgggcg
    13381 cgagccagac gctgatcctg atgttgcacg tgctgcagtt cgtcatcatc ggattgatcg
    13441 ccagcgcgat cggctgcgcc atcggtttcc tgtgccagta cctgctcgcg gcgctgctcg
    13501 cgcccatcgt ctcggtaacc ttgcctgcgc cgggctggtt gccggtgctg caagggctga
    13561 tcgcggggtt cgtcctgctg ctcggctttg cgctgccgcc gctgatcgcg ctcaagaaag
    13621 tgccgaccct gcgggtgctg cgccgggatc tgggcgcgcc ggactcgttc gggttgtcgg
    13681 cttatctgct cggtggcgcc gccatgtcgg gactgatcct gtggcaggcg caggatccca
    13741 agctcgggat gtatgtgctt ggcggggtgg tcggcaccat gatcgcgtcg gctgtcctga
    13801 cactgctggt actgcgcctg ctgtcgtctt tcagccgcgg cgcgcgactc aactggcgct
    13861 tcgggctcgc caacctgcgc cgccgcacca tgggcacggt gatccaggtg atcgcgctgg
    13921 ggttgggcat gatggcgttg atcctgctca cgctggtgcg ttcggacctg ctggaaagtt
    13981 ggaagaggag cctgccgccg gatgcgccga atcgatttct ggtgaacatc cagcccgacc
    14041 aggtcgggga aatcgcggat ttttttgccg tccggcactt gcaccggccg gcgctgttcc
    14101 cgatgattcg cggccgcctc gtggaagtta acggcaagcc ggtatcctcg aaggactacg
    14161 tcgaggacca ggcgcggcga ctcatcgacc gcgagttcaa cctgtcgtgg gcggaaaaaa
    14221 tgcagccggg taacgtgatc gtcgccggcc gctggtttgg cgcctcggat gccggcaagc
    14281 cgttgctgtc ggttgaagaa ggcatcgcca aaacgctgag cctgaagcta ggggatgacc
    14341 tgacctacga catcggcgga tcgcgcctga ccgcgaagat cagctcgctg cgcacggtgg
    14401 actgggactc gttccgggtg aacttcttcg tgatcgcccc cccgggcgtg ctggagcgct
    14461 atcccggcag cttcgtgacc agcctgtacc ttccagcctc gcgatcggtc gtgatggacg
    14521 acatcgtgaa gcgcttcccg aatctgctgg tgatcgacgt ggcggcgatc ctggcacagg
    14581 tgcaacgcat gatggatcag gtggtgcggg cgatcgagtt cgtgttcctg ttcagccttg
    14641 cggccggact gctggtgctg tttgccgcgg tcagcagcac tcacgacgaa cggctgttcg
    14701 atgcggcg
//
LOCUS       DASQOP010000275         3263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2151459, whole genome shotgun sequence.
ACCESSION   DASQOP010000275 DASQOP010000000
VERSION     DASQOP010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2151459"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="VE030_10845"
     CDS             <1..806
                     /locus_tag="VE030_10845"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: protein motif:HMM:NF006599.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HZF23946.1"
                     /translation="ERILAQSSRNLRIEVLKPESMLGIIGGTGLTQLSNLEITRRQVV
                     RTPYGEASGALTFGRLGGREVVFLARHGYGHTIPPHAVNYRANIWALHSEQVSRVVSV
                     ASVGGIRADLKPGTLALPSQIVDYTYGRKHTFFDGPDQPVTHVDFTHPYCDKLRGQLL
                     DAARRSKQAIVDGGVYAATQGPRLESAAEIDRLERDGAHMVGMTGMPEAALARELGLR
                     YATVAVVVNYAAGRGDSANAIHLEQINAVIKTTMVKVRTLLEKLTGSDD"
     gene            799..1329
                     /gene="def"
                     /locus_tag="VE030_10850"
     CDS             799..1329
                     /gene="def"
                     /locus_tag="VE030_10850"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HZF23947.1"
                     /translation="MIKPVLKMGDPRLLERAAPVVQFDTPELHALVQDMEETMRAKNG
                     AGIAAPQIGISQQVVIFGVGFNPRYPDAEEVPYTVLINPVLEPIGTEMEEGWEGCLSV
                     PGMRGVVPRYAKLRYQGYDQFGNPIDRTVTGFHARVVQHETDHLMGILYPMRIRDLRN
                     FGFNEELFPGQQIVDE"
     gene            complement(1330..>3263)
                     /gene="glnD"
                     /locus_tag="VE030_10855"
     CDS             complement(1330..>3263)
                     /gene="glnD"
                     /locus_tag="VE030_10855"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HZF23948.1"
                     /translation="GRSWGDLTKQRILTREEARQLLRHGSALAELRVRLHYLASRRED
                     RLLFDYQSALAKEYGLSDTPARRASEQLMQRFYSTAKQVQLLNTIVLQNLRGRIFPSV
                     DAEPRPLNERFRVRNELLEAAHALFQKQPSAILEAFLLLQDHPELKGFAVPTMRALWH
                     ARGKIDAAFRRDPVNRTLFMEILRRPSGLTHTLRRMNQHGVLGPYIPAFGRIVGRMQH
                     DLFHVYTVDEHILMVVRNLRRFAIPEMAHEYPLCSRLFAEFERPEVLYLAGLFHDIAK
                     GRGGDHSKLGMADSRRFCKAHGLSKEDTDLVAWLVEQHLTMSATAQKQDLSDPDVIGA
                     FAAKVGDDRHLVALYLLTVADIRGTSPKVWNAWKGKLLEDLFRVTRRLLNPAALPAEN
                     TLQARQERVLEKLRAYAIPEGSQRDLWKQLDDSYFLRHDEQDIVWHTRLLNFRVNSPT
                     PVVKARLSPIGEGLQVMIYSRDEKSLFARICGFFQSINFSIVEAKIYTTRHGYALDSF
                     QVMDPARATLHYRDLISYVEHELESRLRAHGPLPPPSTGRMSRQVRYVPITPEVHIRP
                     DEKGAYYYLNVVAGDRPGLLYRIARLLDSYGVDVYTAKINTLGERAEDSFLVTGEALH
                     DSKQVVRLEAELVRELQPA"
BASE COUNT          617 a          995 c         1042 g          609 t
ORIGIN      
        1 gtgaacgaat actggcgcaa tcttcccgca atctacgcat tgaagtcctg aaacctgaat
       61 ccatgctcgg cataatcggc ggcaccggac ttacccaact ctccaatctg gagatcacgc
      121 ggcgtcaggt agtgcgcacc ccctatggcg aggcttccgg cgcgctgact ttcggcagac
      181 tcggcggtcg cgaagtcgtg ttcctggccc gtcacggcta cgggcacact attcccccgc
      241 atgcggtaaa ttaccgggcc aatatctggg cgctgcattc ggaacaggtg tcgcgggtgg
      301 tctcggtggc ctcggtaggc ggcatccggg cggacctgaa gccgggcacg ctggcgctcc
      361 ccagccagat cgtcgattac acctatgggc gcaagcacac tttcttcgac ggtccggatc
      421 agccggtcac gcatgtggat tttacccatc cttactgcga caagctgcgt ggccagttgc
      481 tggatgccgc aaggcggtca aaacaggcaa tcgtggatgg aggcgtctat gccgctacgc
      541 agggtccgcg cctggaatct gccgcagaga tcgacagact cgagcgcgac ggcgcccaca
      601 tggtgggaat gaccggaatg ccggaagcgg cgctggcgcg cgaactcggc ttgcgctacg
      661 ccaccgtcgc ggtggtggtg aactatgctg cagggcgcgg cgacagcgcc aatgcgatcc
      721 atctggagca gatcaatgcg gtgatcaaga ccaccatggt gaaggtgcgg accctgctgg
      781 agaaactgac gggaagcgat gattaaaccg gtattgaaga tgggcgaccc gcgcttgctg
      841 gaacgcgcgg cgccggtggt ccaattcgat accccggagc tgcatgcgct ggtccaggac
      901 atggaagaaa ccatgcgtgc caagaatggc gcgggtatcg ccgcgccgca gatcggcatc
      961 agccagcagg tggtgatctt cggggtcggc ttcaatccgc gttacccgga cgccgaggag
     1021 gtgccttaca cggtgctgat caatccggtg ctcgaaccga tcggaaccga aatggaagag
     1081 gggtgggaag gctgcctgtc ggtgccgggg atgcgcggag tcgtgccgcg ctacgccaaa
     1141 ctgcgctacc agggttacga ccagttcggc aacccgatcg accgtaccgt caccggcttt
     1201 cacgcgcggg tggtgcagca cgaaaccgac cacctgatgg gcatccttta cccgatgcgc
     1261 atccgcgacc tgcgcaattt cggcttcaac gaagagcttt ttcccggcca gcagatcgtc
     1321 gacgaatgat caggcgggtt gcagttcacg caccagctcg gcctcgagcc ggaccacctg
     1381 cttggaatcg tgcaacgcct ctccggtcac caggaagctg tcttcggccc gctctcccag
     1441 tgtgttgatt ttcgcggtgt agacatcaac accatagctg tcgagtaggc gcgcaatccg
     1501 gtacagcagg cctggacggt cgcccgccac cacgttcaga taataataag cgcctttttc
     1561 gtcgggacgg atatgcactt ccggcgtgat cggcacgtag cgcacctggc gtgacatccg
     1621 gccggtcgag ggcggtggca acgggccgtg cgcgcgcagc ctgctttcca gctcgtgctc
     1681 gacatagctg atcaggtcgc ggtaatgcaa cgtcgcccgc gccgggtcca tgacctggaa
     1741 gctgtcgagc gcgtagccgt gcctggtggt gtagatcttg gcttcgacga tgctgaagtt
     1801 gatgctctgg aagaagccgc agatgcgtgc gaacagcgac ttctcgtcgc gcgaatagat
     1861 catcacctgc aagccctctc cgatcgggga aagccgcgcc ttgaccacag gcgtcgggga
     1921 attcacgcga aagttgagca gccgggtgtg ccagacgatg tcctgctcgt cgtggcgcag
     1981 aaagtaggaa tcgtcgagct gtttccagag atcgcgctgc gaaccctcgg gaatcgcata
     2041 ggcgcgtaat ttctccagca ctctctcctg gcgcgcctgc aaggtgtttt cggcgggcag
     2101 cgcggccggg ttgagcaacc gccgcgtgac ccggaacagg tcttccagaa gcttgccctt
     2161 ccaggcgttc cagaccttcg ggctggtgcc gcgtatgtcg gcgacagtca gcaggtaaag
     2221 cgccaccagg tgacggtcgt cgcccacttt cgctgcaaat gcgccgatca cgtcggggtc
     2281 ggacagatcc tgtttctgcg cggttgccga catggtgaga tgttgttcca ccagccaggc
     2341 caccaggtcg gtgtcctcct tcgacagccc gtgcgccttg cagaacctgc gggaatccgc
     2401 cattcccagc ttcgagtggt cgccgccgcg gcccttggcg atgtcgtgaa acagcccggc
     2461 aaggtaaagc acttcagggc gctcgaactc cgcgaacagc cggctgcaga ggggatactc
     2521 gtgcgccatc tccggaatag caaagcgtcg caggttgcgc actaccatca ggatatgctc
     2581 gtccacggta tagacgtgga agaggtcgtg ctgcatgcgt ccgacgatgc gcccgaatgc
     2641 gggaatgtaa ggcccaagca cgccatgctg gttcatccgc cgcagcgtgt gagtaagtcc
     2701 cgaagggcgg cgcaggatct ccatgaacag tgtgcgattc accgggtcgc ggcgaaatgc
     2761 cgcgtcgatt ttcccgcgcg cgtgccacaa cgcccgcatc gtcggcaccg cgaagccctt
     2821 gagttcggga tggtcctgca gcagcagaaa ggcttcgaga atggccgagg gctgtttctg
     2881 gaacagggcg tgggccgctt ccagaagttc gttgcgcacg cgaaagcgct cgttcagcgg
     2941 tctgggttcg gcatccaccg aggggaaaat gcggccccgc aggttctgca gcacgatggt
     3001 gttcagcagc tgcacctgct ttgcggtgct gtagaagcgc tgcatcaact gctcgctggc
     3061 gcgtctggcc ggcgtatccg aaagcccgta ctccttggcc agcgcggact gataatcgaa
     3121 cagcaggcgg tcttcccgcc gcgaggccag gtaatgcagc cgcacccgca attcggcaag
     3181 tgcggacccg tggcgtagca gttgccgcgc ttcttcgcgc gtcaggatgc gctgtttggt
     3241 gagatcgccc caactgcgcc cca
//
LOCUS       DASQOP010000276         6788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_336159, whole genome shotgun sequence.
ACCESSION   DASQOP010000276 DASQOP010000000
VERSION     DASQOP010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6788
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336159"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VE030_10860"
     CDS             <1..492
                     /locus_tag="VE030_10860"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZF23949.1"
                     /translation="LEPAVVSIGAIKGGVRDNIIPDAVEMLGTVRTFNEDMRADIHAR
                     IRNTVEMIARSAGAAAQVHFDNAYPVTVNDVPLTERMVPTLQRVAGADKVFVGQKITG
                     YEDFSFYQQKIPGFFYFLGITPRGTDRKQSAPNHSPRFFVDESALVLGVRSLAHLTLD
                     YMR"
     gene            498..1013
                     /locus_tag="VE030_10865"
     CDS             498..1013
                     /locus_tag="VE030_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZF23950.1"
                     /translation="MPDFSRGNNVITIRRFAAATMLLGAALVPTALLRADSDPEGFVR
                     VQPEDVQWKDLPGYEGVKFAVISGDPSKPGVYVVRVKFPPGIMSRPHFHPDDRYATVL
                     QGTWWTGTGDTFDPSKTVPVKAGGFMKHPAGGHHYDGAKDEEVILQLIGYGPGGTTMI
                     RPEDGHTGPSR"
     gene            1103..2779
                     /locus_tag="VE030_10870"
     CDS             1103..2779
                     /locus_tag="VE030_10870"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HZF23951.1"
                     /translation="MLSDIEIAQQARLKRISALALERLGIPEENLEPYGHYKAKISLE
                     YLDSLKGRPDGKLILVTAISPTPAGEGKTTTTVGLGDALNRIGKKALIALREPSLGPV
                     FGMKGGAAGGGYAQVVPMEDINLHFTGDFNAIQLANNLLAALLDNHIHHGNELGVDVR
                     RITWKRVLDMNDRALREIVVALGGPANGYPRRDAFDIVAASEVMAIFCLSQSLADLKE
                     RLGNIVVGYTRDQKPVRARDLGAQGAMTVLLRDALKPNLVQTLENNPAIIHGGPFANI
                     AHGCNSVIATKAGLKLADYVVTEAGFGADLGAEKFVDIKCRKSGLRPDACVVVATVRA
                     LKYHGGAELKELDKENLAALEKGIANLERHVNNVRNHYGLPCVVSINHFSHDTPAEIE
                     LLKRKMAHHEAPIVVAKHWAQGAAGAEALARTVVDLIERAPADFRFVYEDSATLWEKA
                     RSIATRIYGAADISADTKVKAQIQKLQEDGYGHYPVCMAKTQYSFSTDASVRGAPSGH
                     IINIREVRLAAGAEFVVMVCGDIMTMPGLPKVPSATKIDLTPDGKVVGLF"
     gene            2879..4675
                     /locus_tag="VE030_10875"
     CDS             2879..4675
                     /locus_tag="VE030_10875"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZF23952.1"
                     /translation="MSSQFKSATALSIQAGTSTAPQDLHWLEKNIPCQAACPAGTDIP
                     GYLEAIYHGRFAEAYEINLRDNVFPAVLGRVCSRPCEDACRHGWEGNGEPVAICFSKR
                     SAADFGASAPVVLEPDFPASGKRVAVIGAGVAGLTAARELALSGHGVTVFEKHRTPGG
                     MLNQGIPAFRLPREIVDREIRQVAATGVIICCGVEIGTDVTLARLSEDFDAVVLAAGT
                     LRPNILELPGKELTGIEHGLSFLLQVNEFGRRDIGGRVIVIGGGYTAMDCARTALRLG
                     AQVGVVYRRAQEDMVVLPGELEELLEEGGHLETNCAPLRFLGDRGKVIGVRFVRTRQG
                     EPGRDGRRAPAEIPGAELAIGADTVILATGQFPQAAWIDAELAPTLVARDGWLASGTA
                     CQTAQRNVFVAGDFALGATTLIQAIGHAKECARKVDAWLTGRPRTEQVAQLGAAFRSK
                     LPGGRTTGRTAEMNVIPIHPMPTLAVAARGLEAEVETGFAEPVAKEEASRCYLCHYKF
                     EIVDRDCVLCDECLKVKPVEGCIVEIASLLRDEEGRILSYTKVEKGGTDSLYYNRLWI
                     DQSQCIRCGRCEAVCPVNAITIQKVTLAATPA"
     gene            4888..5427
                     /locus_tag="VE030_10880"
     CDS             4888..5427
                     /locus_tag="VE030_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HZF23953.1"
                     /translation="MAGTPSRWLMVEEAISYYARRMVAWSHGEVVATYHGGVSRKTGA
                     RSQLSTHSIIAIKGAVDFSKDYHAEPHLSNTSLFFRDRHMCAYCGGQYKERDLSRDHV
                     MPVYLGGKDRWMNVVTACRGCNIRKGGRTPESADMPLLYTPYVPNRYEHLILQNRRIL
                     QDQMEYLMAKVPKHSRLYG"
     gene            5574..6443
                     /locus_tag="VE030_10885"
     CDS             5574..6443
                     /locus_tag="VE030_10885"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HZF23954.1"
                     /translation="MKKALFTLAFAVLALCACQTNPITGRQQLMLVSEDMAISESKQA
                     YVAMLQPLQQKGKIDSNPAVSARVRMITGRLIAQAIRYRPETESWEWSVKVIDDPKTV
                     NAWCMAGGKMAIYTGLIEQLKATDDELAQVMGHEISHALAKHTAERMSRALAMQMGLG
                     VLAATQQSSRYGNMTLTGAQMAAVVALELPNSREAESEADRIGIELAAKAGYDPNAAV
                     SLWDKMAKLGSGSSRLDFLSTHPAPQKRMETLAALVPKMMPYYLDKSPRPIYQIRASA
                     SARAVLAQSATAR"
     gene            complement(6452..>6788)
                     /locus_tag="VE030_10890"
     CDS             complement(6452..>6788)
                     /locus_tag="VE030_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23955.1"
                     /translation="DGKLVDRTILLDTGRIRVTGKGSADFGTETINLRMRPQTKTAQF
                     LSLATPIQVSGTFRNFKISVSPGDVLETIGRLATSIIWVPLQKIAGKKIPRDGSDVCG
                     ESLLTLPVP"
BASE COUNT         1352 a         2064 c         2179 g         1193 t
ORIGIN      
        1 ctggagccgg cggtggtcag catcggcgcg atcaagggcg gggtgcggga caatatcatt
       61 ccggacgcgg tcgagatgct gggtacggtg cgcacgttca acgaggacat gcgcgcggac
      121 attcacgccc gcatccgcaa tacggtggag atgatcgcca ggagtgctgg ggcagccgcg
      181 caggtgcatt tcgacaacgc ctacccggtc acggtgaacg acgtaccgct gaccgagcgc
      241 atggtgccga ctctgcagcg ggtggcgggc gcagacaaag tcttcgtcgg ccagaaaatt
      301 accggttacg aggacttctc cttctatcag cagaagattc cgggattttt ctatttcctc
      361 ggcatcacgc ccaggggcac agatcgcaaa cagtcggcgc cgaaccattc accgcgtttc
      421 ttcgtcgatg aatctgcgct ggtgttggga gtacgctcgc tggcgcacct cacgctcgat
      481 tacatgaggt aaacttcatg ccagactttt cgcgggggaa taacgtgatc actattcgca
      541 gattcgccgc tgcaaccatg ctgctgggtg cggcgcttgt ccccacggcg ctgctgcgcg
      601 cggactccga tccggaggga ttcgtgcgcg tgcagccgga ggacgttcaa tggaaagacc
      661 tccccggcta cgagggcgtc aagtttgcgg tgatttcggg cgacccgtcc aagccgggtg
      721 tctatgtggt ccgggtgaag ttcccgcccg ggatcatgtc gcgcccgcac ttccatcccg
      781 atgaccgcta cgccaccgtc ttgcagggca cctggtggac cggcaccgga gacaccttcg
      841 atccaagcaa gaccgttccg gtcaaggcgg gaggcttcat gaagcacccg gcgggaggtc
      901 accattacga tggcgccaag gatgaagagg tgattctgca gctcatcggg tacggtccgg
      961 gcggcaccac catgatccgc cccgaagacg gacataccgg cccgtcgaga taagaacagg
     1021 gatttccgca aggagcggca gatttctctg ccgctttttt tgttttgctc tcgcggaccg
     1081 gcaaaaatca caagaggtca ccgtgctttc cgatattgaa atcgcccagc aggccaggct
     1141 caagcgcatc tccgcgcttg ccttggaacg tctgggaatc cccgaagaga atctcgaacc
     1201 ctacggtcac tacaaggcga agatctcgct tgagtatctg gacagcctga agggccggcc
     1261 ggacggcaag ctgatcctgg tcaccgccat cagccccacg ccggcgggcg agggcaagac
     1321 caccaccacg gtgggattgg gcgacgcgct caaccgcatc ggcaagaaag cgctgattgc
     1381 attgcgcgaa cccagcctcg gcccggtatt cggtatgaaa gggggcgctg ccggcggcgg
     1441 ctatgcgcag gtggtgccga tggaagatat caacttgcac tttaccggcg atttcaacgc
     1501 catccagctc gccaataatc tgctggcggc gctgctcgac aaccacatcc accacggcaa
     1561 cgaactcggg gtggacgtgc gccgcatcac ctggaaacgg gtgctggaca tgaacgaccg
     1621 cgcgctgcgc gagatcgtgg tcgcgctcgg cggtcccgcc aacggttacc cgcgccggga
     1681 cgccttcgac atcgtggcag cctcggaagt gatggcgatc ttctgcctgt cgcagtcgct
     1741 tgccgacctg aaggaaaggc tcggcaatat cgtggtcggt tatacgcgtg accagaagcc
     1801 ggtgcgggcg cgcgacctgg gagcgcaggg ggcgatgacg gtgctgctca gggatgcgct
     1861 caagcccaat ctcgtgcaga cgctggagaa caatccggca atcattcacg gcggaccgtt
     1921 tgccaatatc gcacacggct gcaactcggt cattgccacc aaggcgggat tgaagcttgc
     1981 cgactatgtg gtgacggagg cgggatttgg cgccgacctg ggcgctgaga aattcgtgga
     2041 catcaagtgc cgcaagtccg gcctgcgccc cgacgcctgc gtggttgtcg ccaccgtgcg
     2101 cgcgctcaag taccacggtg gggcggagct aaaggaactc gacaaggaaa atctcgccgc
     2161 gctggagaag ggcattgcca atctcgagcg gcacgtcaac aacgtgcgca accattacgg
     2221 cttaccgtgc gtggtgtcga tcaaccactt cagccacgac acgccggccg agatcgagtt
     2281 gctcaagcgc aagatggcgc accacgaagc gccgatcgtg gtggcgaagc attgggcgca
     2341 gggcgcagcc ggtgccgaag cgctcgcgcg cacggtggtg gatctgatcg agcgcgcacc
     2401 ggccgacttc cgcttcgttt acgaggattc ggcgacgctt tgggaaaagg cgagatcgat
     2461 cgccaccagg atctacggcg ccgccgatat cagcgcggac accaaggtca aggcgcagat
     2521 ccaaaaacta caggaggacg gctacggcca ctacccggtc tgcatggcca aaacccagta
     2581 ttcgttttcg accgatgctt cggtgcgcgg cgccccgtcg ggccacatca tcaacatccg
     2641 tgaagtgcgg ctcgccgccg gagcggaatt cgtggtcatg gtctgcggcg acatcatgac
     2701 catgccgggc ttgccgaaag tgccttcggc gaccaagatc gacctcacgc ccgacggtaa
     2761 agtggtgggg ttgttctgaa aggccgtgac gacgtgactg ccgtaactat cgtgactagg
     2821 gcaccgtcct tggtttctgc ggtagtcacg cagtcacgat agtcacgcag tcacgatcat
     2881 gtcatctcaa ttcaaatcag cgacggccct gtccatccag gctggtacca gcaccgcacc
     2941 ccaggatctt cactggctgg aaaagaatat cccgtgccag gcggcatgtc cggcgggcac
     3001 cgacatcccc gggtatctcg aggcgatcta ccacgggcgg ttcgcagaag cctatgaaat
     3061 caacctgcgt gacaatgttt tccccgcagt gctcggccgg gtgtgctcgc ggccgtgcga
     3121 ggacgcctgc cgacacggct gggaaggcaa tggcgagccg gtggcgatct gcttttccaa
     3181 gcgctcagcc gcggatttcg gcgccagcgc accggttgtc ctcgagccgg attttcccgc
     3241 cagcggaaaa cgggttgcgg tgatcggcgc cggcgtggcg gggctcacag cagcgcgtga
     3301 actggctctc tccgggcacg gcgtgaccgt gttcgagaag caccggactc ctggcggaat
     3361 gctcaatcag ggcatcccgg cgttccgcct gccgcgcgaa atcgtcgatc gcgagattcg
     3421 ccaggtcgcc gccaccggcg tgatcatctg ctgcggagtc gaaatcggta ccgacgtcac
     3481 gctggcccgg ctctctgaag acttcgacgc ggtggtgctg gccgcgggaa cgctgcggcc
     3541 caacatactt gagctgcccg gcaaggaatt gacgggaatc gagcacggac tgagtttcct
     3601 gctgcaggtg aacgagttcg gcagacgtga catcggcggc cgagtgatcg tgatcggggg
     3661 tggctatacg gccatggact gcgcccgtac cgcgctgcgg ctaggcgcac aagtcggcgt
     3721 cgtctatcga cgggcgcaag aagacatggt ggtgctgccg ggagaactcg aggagttgct
     3781 ggaggagggt gggcacctgg aaacgaactg cgcgccgctc cgctttcttg gcgatcgggg
     3841 taaggtgatc ggcgtgcgtt tcgtgcgtac caggcaggga gaacccggcc gcgacggccg
     3901 gcgcgcgccg gccgagatcc ccggagccga actcgcgatc ggcgccgaca ccgtgatcct
     3961 cgcgaccggg caatttccgc aggcggcatg gatcgatgcg gaacttgcgc cgacgctggt
     4021 ggcgcgcgac ggctggctgg cgagcggcac ggcctgccag accgcgcagc gcaatgtttt
     4081 tgtcgcaggt gattttgcgc ttggcgccac tacgctgatt caagcaatcg gacatgcaaa
     4141 ggaatgtgcg cgcaaggtgg acgcatggct gacggggcgg ccgcgcacgg aacaggtcgc
     4201 gcaactcggc gccgctttcc gctcgaagct gccgggcggc cgcaccaccg ggcgcaccgc
     4261 ggagatgaac gtcattccga ttcatccgat gcctacgctt gcggttgcgg ccagggggct
     4321 ggaggcggaa gtcgagaccg gattcgccga gccggtggcg aaggaagagg cgtcgcgctg
     4381 ctacctttgc cactacaaat tcgagatcgt ggaccgggac tgcgtgctgt gcgacgaatg
     4441 cctgaaggtg aagcctgtgg aaggctgcat cgtcgagatc gcttcgctgc tgcgggacga
     4501 agagggacgg attttgagct ataccaaagt ggaaaagggc gggaccgact cactttatta
     4561 caaccgcctg tggatagacc agagccaatg tattcgctgc ggccgttgcg aggcggtatg
     4621 cccggtcaac gccattacca tccagaaagt gactctggcc gccaccccag cctgacgggc
     4681 cggggaatca cccgcataaa cgggtgctta gacggcgcag atgcttccaa actgcgtttg
     4741 atctgcctat actcctgaaa acgccgcgtt tccggccttt cgattacaag ttgcgtccgg
     4801 aatccggccc aaacatcgaa gcctgaccat aagtcagagc taggactgtc caggagagcg
     4861 gagttggccc tgattcttgc agtggatatg gccggcaccc ccagccggtg gctgatggtc
     4921 gaagaggcca tctcttacta cgcgcgccga atggtggcgt ggtcgcacgg cgaagtcgtg
     4981 gcgacctacc atggcggcgt gtcgcgcaag accggcgcgc gttcccagtt gtccacccac
     5041 agcatcatcg ccatcaaggg cgcagtggac ttctccaagg actatcacgc cgagcctcat
     5101 ctgtccaata cctcgctgtt tttccgcgac cgccacatgt gcgcctattg cggcgggcag
     5161 tacaaggaac gcgacctgtc gcgcgaccac gtgatgccgg tctatctcgg cggcaaggac
     5221 cgctggatga acgtggtgac cgcctgccgc ggctgcaaca tccgcaaggg cgggcgcacc
     5281 ccggaatcgg ccgacatgcc gctgctgtac acgccctacg tgcccaaccg ctacgagcat
     5341 ctgatcctgc aaaaccgccg catcctgcag gaccagatgg aatacctgat ggcgaaggtg
     5401 cccaagcaca gcaggctgta cggctgagct gcggctgcga cgcaaaccaa gccggcacct
     5461 gctgccggct tttttgcgtc tgttcgcacc gcaaagtggg tcagcgcacg gaactgcaag
     5521 gcgttacgat ggacaatacg cactttccgg aaccaggcag aggaccgcat ccaatgaaga
     5581 aggcattgtt caccctggcg ttcgcagtgc tcgcgctgtg cgcctgtcag accaatccga
     5641 taaccggtcg ccagcagttg atgctggtat ccgaggacat ggccatctcg gaatcgaaac
     5701 aggcctacgt cgcgatgctt cagccgctgc agcaaaaggg caagatcgac agcaacccgg
     5761 cggtgagcgc gcgggtgcgg atgatcaccg gacggttgat cgcgcaggcg atcagatatc
     5821 ggccggaaac agaaagctgg gaatggagcg tcaaggtgat cgacgacccc aaaaccgtca
     5881 acgcctggtg catggccggc ggcaagatgg cgatctatac cggcctgatt gagcagctca
     5941 aggcaaccga cgacgaactg gcgcaggtga tgggccacga aatctcgcac gcgctggcga
     6001 agcacaccgc ggaaaggatg tcccgtgcgt tggccatgca gatggggctg ggcgtgcttg
     6061 ctgccaccca gcagtccagc cgctacggca atatgacgct caccggtgca cagatggctg
     6121 ccgtggtcgc gctggaattg cccaacagca gggaggccga atcggaggcc gatcgcatcg
     6181 gcatcgagct ggcggcgaaa gccggctacg atccgaacgc agcggtgtca ctgtgggaca
     6241 agatggcgaa actcggaagt ggcagttcac gcttagattt cctttccact cacccggcgc
     6301 cgcagaagcg catggaaacg ctcgccgcac tggtgccgaa gatgatgccg tactacttgg
     6361 acaagtcccc gcgcccgatt tatcagatac gcgcgtccgc cagtgcgcgc gcggtcttag
     6421 cgcagtccgc aacagcgcgg taaccgcgta ctcagggcac cggcagcgtc aggagcgact
     6481 caccgcatac gtcgctgccg tcacgcggaa tcttcttgcc ggcaatcttc tgcagcggca
     6541 cccagatgat cgaagtcgcc agccggccga tcgtttcgag cacatcgccg ggagagacgc
     6601 tgatcttgaa attcctgaac gtgccgctga cttggatcgg cgtggcgaga ctcaggaact
     6661 gcgcggtctt cgtctgaggg cgcatgcgca ggttaatcgt ttcggtaccg aaatccgcac
     6721 tgcccttgcc ggtaacgcgt atgcgcccgg tatcgagcag gatggtccgg tccaccagct
     6781 tgccgtca
//
LOCUS       DASQOP010000277         2677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1347538, whole genome shotgun sequence.
ACCESSION   DASQOP010000277 DASQOP010000000
VERSION     DASQOP010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1347538"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..152
                     /locus_tag="VE030_10895"
     CDS             <1..152
                     /locus_tag="VE030_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HZF23956.1"
                     /translation="QRRVPLQNGDVVIWGGPARLHHHGVLALKEGHHDLLGAQRLNLT
                     FRRAA"
     gene            complement(230..1846)
                     /locus_tag="VE030_10900"
     CDS             complement(230..1846)
                     /locus_tag="VE030_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZF23957.1"
                     /translation="MSNESAIRREVGAIALMFTGLGSIIGSGWLFGAWRAAQLAGPGA
                     VYSWAIGAVIILFVAFTYAELGAMFPESGGAVRYGHYSHGSLVGFVAGWAAWIAIVSV
                     IPVEAEASVQYMSSWPWGWAQHLYVHAANGQGELSAAGLSISAVLVVLYFLVNFWSVK
                     VFADTNSAITFFKLIVPAATAVALMFSGFHWQNFQVGIHGGRHVGSVAAILTAVATSG
                     IVFSYNGFQSPVNLAGEARNPGRSVPIAIFGSIALSTVVYLLLQVAFIGAVAPEHLQE
                     GWAGLEYSSPFAQLALALNLNWLALLLYADAFVSPSGTGTTYTATTARMIYAMERSDT
                     VPEVFGRVHPRFGIPRPAMWFNLAVSFIFLFFFRGWGKLAAVISVATIITYLVGPISV
                     MVLRRTAPNLHRPLRVPGLPLLAPIAFVLATLMLFWARWPHTGQIMLLLILPMPVYLY
                     YQAKSNWLNFGRQLRGSWWLLAYLIVITVLSWAGSKEFEGHGYIGYGWDQLCVVLAAL
                     VFYFWGLRSGWRTPAVEAVEGGAKTPDTIA"
     gene            1916..2422
                     /locus_tag="VE030_10905"
     CDS             1916..2422
                     /locus_tag="VE030_10905"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HZF23958.1"
                     /translation="MGNRRRIPVSLNIPVKNWLYRRIIEPLLALLRQGISPDRLALCV
                     AIGVVVGNIPILGISTILCTVIALTFRLNLPAIQIVQALMAPTQLLLIIPFVRLGEWI
                     LRVPAQPVSIEKGLELAARGAGYAIVALWDAILHAGFAWILVAPVAVLLFYKLLTPVF
                     EHTAAQIK"
     gene            complement(2432..>2677)
                     /locus_tag="VE030_10910"
     CDS             complement(2432..>2677)
                     /locus_tag="VE030_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HZF23959.1"
                     /translation="SSAVMSYLADQRGVTDVHDLHIWALSTTSVALTAHLVVPDRGAD
                     DALLDSITPNLKRRFHIHHATLQIERDRCEHGCEAEA"
BASE COUNT          564 a          833 c          821 g          459 t
ORIGIN      
        1 agcagcggcg ggtaccgctg cagaatggcg acgtcgtcat ctggggcggg cccgcgcgcc
       61 tgcatcatca cggcgtgctc gcgctcaagg agggtcatca cgacctcttg ggcgcgcagc
      121 gcctgaatct gacgttccgc cgcgcagcct gatttcacgc acgacgctga gcggtcagaa
      181 aaatcttatg tagcgagcgg cggatgtcac ttccaacacc aatgcgctgt caggcaatcg
      241 tgtcaggtgt tttcgcaccg ccttccaccg cctcgaccgc gggcgtacgc caaccgctgc
      301 gcagacccca aaaatagaac accagggcgg ccagcacgac gcagagttga tcccagccgt
      361 agcctatgta gccgtgacct tcaaattcct tgctaccggc ccaggacagc acggttatca
      421 cgatcaagta ggcgagcaac caccaggaac cgcgcaattg gcggccgaaa ttgagccagt
      481 tcgacttggc ttggtagtag agatacacag gcatcggcag aatcagcagc agcatgatct
      541 ggccggtatg cggccagcgc gcccagaaaa gcatcagcgt ggcgagtacg aaggcgatcg
      601 gcgcgagcag cggcaaaccc ggcacgcgca gcggccggtg caggttgggc gccgtacgcc
      661 ggagcaccat gacgctgatc ggtcccacga ggtaggtgat gatggtggca accgagatca
      721 cggcggcgag ctttccccaa ccgcgaaaga agaacagaaa gatgaaggac accgccaggt
      781 tgaaccacat cgcaggccgc gggatcccga agcgcggatg aacacggccg aatacctccg
      841 gcacggtgtc gctgcgctcc atggcgtaga tcattcgcgc agtggtcgcc gtgtacgtgg
      901 tgccggtgcc gctcgggctc acgaaggcat cggcgtaaag caggagggcg agccagttga
      961 gattcaacgc cagcgcgagc tgtgcgaacg gcgagctgta ttccagcccg gcccagcctt
     1021 cctgcaggtg ttccggcgcg acggcgccga tgaatgcaac ctgcagcagc agatacacca
     1081 ccgtgctcag cgcgatcgac ccgaagatgg cgatgggcac gctgcgcccc gggttgcggg
     1141 cctcgcccgc caggttcacc gggctctgaa agccgttgta gctgaatacg atgccgctgg
     1201 tggcgaccgc ggtcagaatg gcagcgacgc ttccgacgtg ccggccgccg tgaatgccca
     1261 cttggaagtt ctgccaatgg aagccggaaa acatcagcgc cacggcagtc gccgccggca
     1321 cgatcagctt gaaaaaggta atggcgctgt tggtatcggc aaaaaccttc acgctccaga
     1381 aattgaccag aaaatagagc accacgagta cggcggaaat ggatagaccg gccgctgaca
     1441 actctccctg gccgttggcg gcgtgcacgt ataggtgctg cgcccagccc cacggccagg
     1501 aactcatgta ctgcaccgat gcttcagctt ccaccgggat caccgagaca atggcgatcc
     1561 aggcggccca tccggcgaca aacccgacca acgagccgtg cgaataatgt ccatagcgca
     1621 ccgcgcctcc ggactccgga aacatggcgc cgagttcggc ataggtaaac gcgacgaaaa
     1681 ggatgatcac agccccgatg gcccacgaat agacagcgcc gggaccggcc agctgggcgg
     1741 cgcgccaggc accgaacaac cagcccgacc cgatgatcga acccaagccg gtgaacatca
     1801 acgcgatggc gccgacttcg cggcgaatcg cactttcatt ggacatgcgt ctttctccct
     1861 ggcatcgact gtaacggctc ggccgggcct gcgccatgcc gaacccacga attccatggg
     1921 caatcgtcgc cgaatacccg tatcgttgaa catccccgtg aagaactggc tataccgaag
     1981 aattatcgaa cctctcctgg ccttgttgag gcagggcatt tcgccggatc ggcttgcgct
     2041 ctgcgtggca atcggagtgg tggtcggcaa cattcccatc cttggcattt ctaccattct
     2101 ttgcacggtg atcgccctga catttcgttt gaatctaccg gcgattcaaa tcgtgcaggc
     2161 gctcatggcg cccacgcaac tgttgctgat cattcccttc gtccgactgg gcgaatggat
     2221 cttgcgagtc cctgcccagc ccgtatcgat cgagaaagga ctcgaactcg cggcgcgcgg
     2281 cgccggatat gccattgtcg cgctctggga cgcgatactg catgcgggat tcgcctggat
     2341 cttggtcgcg cccgttgccg tcttgttgtt ctacaaattg ttgacgccgg tcttcgaaca
     2401 cactgctgcg cagatcaagt agggcgagac gtcaggcctc ggcttcacac ccgtgttcgc
     2461 agcggtcgcg ctcgatttgc agagtggcat gatgaatgtg gaagcgccgc ttgaggttgg
     2521 gcgtgatgga gtcgagcagc gcatcgtccg cgccgcgatc cggcaccacc aggtgtgcgg
     2581 tcagggcgac gctggtggtg ctgagcgccc agatatgcaa atcgtgcacg tcggtcacgc
     2641 cccgctgatc ggccaagtag gacatcacgg ccgagga
//
LOCUS       DASQOP010000278        18440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2561069, whole genome shotgun sequence.
ACCESSION   DASQOP010000278 DASQOP010000000
VERSION     DASQOP010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 18440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18440
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2561069"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..801)
                     /locus_tag="VE030_10915"
     CDS             complement(22..801)
                     /locus_tag="VE030_10915"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HZF23960.1"
                     /translation="MSRMYACFALAALAASLRPVYAADLTVEQVKQVLSSPERVTTAA
                     LAGKDLSRLDLGRQDFRGVNLVGTNFFASKLVQCNFSGAKLSGANLNGAWLMGTDFSG
                     ADLSRATLMSVVILGGEVTARPNFRNANLSGARIIAELDGADLSEANLSKARLGVDIK
                     NQGMGQMRTNLVKANLGGANLAEADLNRTLLRFANLKDANLRGADLYRADLSGADLTG
                     ADVTGANLEEANVDGTIFKGVRGLDAAKGFDRAEHRDNAKF"
     gene            complement(833..1507)
                     /locus_tag="VE030_10920"
     CDS             complement(833..1507)
                     /locus_tag="VE030_10920"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HZF23961.1"
                     /translation="MNEQQAAVLRQHMVLEIAAQTVLAGERIGKYNLDARVMEVMGKV
                     PRHEFVPVEIQPYAYLNRPLPIGFDKTISQPFIVALMTDMLEIRKEDVVLEIGTGLGY
                     QAAVLAELAHKVYSMEIIEALAQQAVKRLSRAGYTNVEVKVANGSYGHPEHAPFDKVM
                     VTAAPDLIPPPLINQLKPGGRMVIPAGLSDNQQLILVEKSANGRLSTREILPVRFSQL
                     EGAESF"
     gene            complement(1594..2004)
                     /locus_tag="VE030_10925"
     CDS             complement(1594..2004)
                     /locus_tag="VE030_10925"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HZF23962.1"
                     /translation="MPYIEESLSAGEQIVGFFRLHWISRRWMVLWIVLAIPTVGITLI
                     LALFEFLRLRSIEQGVTNKRVIYKKGIISRKSEEMKLNSIETVEIDQGIPGRIFGFGD
                     VTVTGRGLSNVVFKSVDDPMAVKRKIESVSNPIG"
     gene            2117..3334
                     /locus_tag="VE030_10930"
     CDS             2117..3334
                     /locus_tag="VE030_10930"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HZF23963.1"
                     /translation="MPAVSRRANDLGTENAFVVLAEVSALQRQGKDIISFCIGQPDFH
                     TPKNIRDAGIRAINEGKHGYTPSAGILELREAAANYLNRTRAIKVSADDIVVGAGAKP
                     FIAYSILSVTDYGAGDEVVFPNPGFPIYESQIRAHGAVPVPIHLRESRNFSLDPSELE
                     AEITPKTKLLILNSPQNPTGGIIPKKDMEAIAAILRRHPQVWVFADEIYSQLCYDGEF
                     VSLAQFPDMLERTIISDGASKTWAMTGWRLGFTANRALAPVFTRWVTNTDSCASQITQ
                     WAAVEAMNGPQDEAKKMRDSFLARRDLIVRLLNKVPGVSCRNPGGAFYAWPNVTAACE
                     MIGAKDSEEFRKRLLNEAGVAVLADIHFGPRVPNEGQHIRFSYAASNEAIEKGVARMA
                     DFIRKNTRIKKTA"
     gene            3388..3786
                     /locus_tag="VE030_10935"
     CDS             3388..3786
                     /locus_tag="VE030_10935"
                     /inference="COORDINATES: protein motif:HMM:NF042483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HZF23964.1"
                     /translation="MKILNRLLAGFLATSIAFTSLVQAAPTLITTEQVAVEDAQKDRA
                     LVNETFLRPELQAKLRESGVSLQDAQARVAALTDQEISALARQIETAPAGGTDVLTFV
                     LVIFIVLLITDIMGFTTVFPFVKHGSGKAK"
     gene            complement(3976..4293)
                     /locus_tag="VE030_10940"
     CDS             complement(3976..4293)
                     /locus_tag="VE030_10940"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HZF23965.1"
                     /translation="MNLWLVVIAAGVLTFLIRLSFISLLANWAMPPLAQRALSFVPPA
                     VMTAIVFPELLVRGGDLAVGMDNHRLTAGVIAILVAWRFKKIMPTIVAGMAALWLLQY
                     YAG"
     gene            complement(4290..4976)
                     /locus_tag="VE030_10945"
     CDS             complement(4290..4976)
                     /locus_tag="VE030_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HZF23966.1"
                     /translation="MNTARAQFLSGLKDTSPLLIGAAPFGVIYGVLATGAGIPVVMAQ
                     AISLIVFAGSAQFIAAQLIGAGASGLVIVLTTFIVNLRHALYSASVAPYVKDLPRTWK
                     WVLSYLLTDEAFAVAIAHYRDNAGEPSRHWYFLGAGVSLWGTWQASTAVGILLGTEIP
                     HEWSLDFTLALSFIALLVPVLRTRADLVAALVGGIVAVAAFGLPYKLGLILAAFCGIG
                     AGMLAWRLGK"
     gene            complement(5162..6019)
                     /locus_tag="VE030_10950"
     CDS             complement(5162..6019)
                     /locus_tag="VE030_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZF23967.1"
                     /translation="MKEFQDRVAVVTGGASGLGRAMALRFAREGMKIVLADIQADALK
                     KTEADFEQARIPVLAVRTDVSKSKDVEALADKAFKTFGGVHILCNNAGVAPGGTVWEH
                     TEKDWEWTLGVNVWGVIHGIRVFVPRMLEQNVDCHVVNTASVAGLLSLPGMSAYCVSK
                     HSVVTLSECLHHDLVEFGSKLKASVLCPAYVPTGISDSERNRPAALREDKKKNPEDLK
                     REAQLRHAVQSGRISAEQVADLVYEAIKAEKFYILPHQKIKPAIETRMQDILLERAPT
                     DTLNRKSNA"
     gene            6150..7208
                     /locus_tag="VE030_10955"
     CDS             6150..7208
                     /locus_tag="VE030_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HZF23968.1"
                     /translation="MNEEFIGTMPVQERHRFDVVSLERYLRQHIAGFSGNLEVEQFRG
                     GQSNPTFRLTAGGKRFVLRRKPPGKLLPSAHAVDREYRVITALHGSDVPVAKTYCLCE
                     DDAVIGTAFYIMDYIEGRVLWDPSLPGMSTVQRAAYFDELNRVIAALHKVDYARVGLA
                     DYGKPGNYIARQINRWTKQYRASETERIEAMENLIEWLPNNIPPGEETTIVHGDYRMD
                     NVIFHPQEAKILAVLDWELSTLGHPLADFAYHCMTWRLTPGQFRGMNGIDFRALAIPT
                     EQEYVAAYCRRTGRSHIPNWDFYLAYNMFRLAGILQGIMGRVKDGTAASAYAMEQGKR
                     ARPMAEAGWRQVEKILKS"
     gene            7442..8422
                     /locus_tag="VE030_10960"
     CDS             7442..8422
                     /locus_tag="VE030_10960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HZF23969.1"
                     /translation="MKAVLCKELGLPEKLVVEEVPSPKAEPGQVVVSVKACGVNFPDT
                     LIIQGKYQFKPELPFSPGGEVAGVVKEVGDGVTRVKRGDRVIAFNTWGGFAEEIAVDA
                     ERTIPMPLAMDFVPASAFVLTYGTSYHALKDRADLKAGETLLVLGAAGGVGLAAIQLG
                     KAMGAKVIAAASNDAKLQICRQNGADEVINYGTEDLRTRIKAITAGKGVDVVYDPVGG
                     PYSEPALRDMAWNGRFLVVGFAAGDIPKVPLNLTLLKGCSIVGVFWGAFTRNEPERNR
                     ENNYDLMAMYAAAKVRPHIHATYPLERAADALNEVLNKRVTGKVVLVTGK"
     gene            8643..9815
                     /locus_tag="VE030_10965"
     CDS             8643..9815
                     /locus_tag="VE030_10965"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HZF23970.1"
                     /translation="MNEAVIIEAVRTPFGRRGGALREQRPDTLLAHALDGLIKRSQID
                     PQKIEDVIDGTVTLAGEQGANPARLAVLLSGLPVKVPGVSLNRMCGSSQQAVHFAAQA
                     VAAGDMNYVIGCGVESMTRTPMFLDIGLGRTVSGFEVLNPELFQRHELIHQGESAERI
                     SEKWGLSRRMLDDFARESHRRAAKAQKAGSNKELLPVQGVSPEGEAMRLERDEGVRAQ
                     IDEAKMASLKPAFRPDGNGVITAANSSQISDGAAALLVANRDVAKADGFKPRARFRAR
                     IAVGSDPMMQLTGVIPATELALKKAGVSIKNIDWIEINEAFAAVVLSWAKEIHPDMDK
                     VNPWGGAIAHGHPLGATGAGLMSKLLAGLEATGGTLGLQVMCIGHGQATATIIERI"
     gene            10219..11913
                     /locus_tag="VE030_10970"
     CDS             10219..11913
                     /locus_tag="VE030_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HZF23971.1"
                     /translation="MKDKGGMRRNLTNYGDPEFAAYLRRSFARSMGYSKDMLERPIVG
                     IAQSASGFNNCHRYVPELTEAVKRGVLKSGGLPIDFPTISLGEVFLSPTSLMFRNLMA
                     MDVEEMVRAQPMDAVVLIGGCDKTVPAQLMGAASADVPAIQLVTGPMMAMPYRGERLG
                     ACTDCRRFWARFRAGEVSDEGIDEIEGNLATTAGTCAVMGTASTMACVAEGLGMSLPD
                     TAAIPAVHADRIRAAEATGVRAVEMIRTKGPRPSEIITEKSVENAIRVLLAVGGSTNA
                     IIHLTAVAGRRGIGISLERLNRLSDETPVLLDLKPTGSHYMQDFHAAGGMSALLAELK
                     DLLHLDCMTVTGETLGSRIASGVAFVDRGVIRPRSDPISPVGGLVALFGSLAPRGAIL
                     KRAAADSGLFEKEGRAVVFESLEDLSARIDLPDLDVTKDDFLVLKNAGPRSASGMPEA
                     GYLPIPKKLAKTGLKDMVRISDARMSGTAFGTVVLHVAPDAASGGPLALVKSGDRIRL
                     SVRNRRLDLMVAEADLVKRRDGFRTPHLPERGYARLYNEHILQADAGCDFDFLRGS"
     gene            complement(11921..14530)
                     /locus_tag="VE030_10975"
     CDS             complement(11921..14530)
                     /locus_tag="VE030_10975"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZF23972.1"
                     /translation="MTKEPDKQNDPIRILMVEDVSVDAELELRELRRAGLHIAARVVE
                     IETDFRKEIESFDPHVILSDFSLPRFSGMEALSISRELRPDIPFIFVSGTIGEENAVA
                     ALRNGATDYVLKSNLKRFAPAVLRAIKEARERKARLAAERRAKDTEHYFSLFMRHLPA
                     AAFTKDPQGRFTFANPTFEELAGKPDGQLIGLTSHDLYPPDYVEPLVANDRKVVESRQ
                     PYRVVEKVIFGESDRFFLVSKFPIKDESDNVAMIGGVAVDITDRILQERKLERLSRIR
                     EVLSSLTAAIIRLRTPEDLCRELCRIAIDVGHFRMAWAGLVQKDAEGIRPVASHGAVD
                     GFLEVVTGGRDIVRLEQEIPATMAVRAQRQIVIDDIETSDTDPDWKQAALVRGFRSCA
                     ALPLNSGDKVAGVGVLFSEEPHFFDVEHLRLLSDLAADAALALERMEKQDLVEYLSFY
                     DPLTGLANRTLVQERLQQFLHEATRNRHMLALMILDLERMHIVNESLGRGAGDKLLKL
                     VAERLQSQHVPEQVGRIGMNSFIAVLPDIRDGIHAARLVEEGRKNPFSRPFDLDGNDI
                     RVSARCGVAVFPADGQDSETLIRNAEAALTQSKQTGDATVYYTPTLNADFAQRLALET
                     KLRRAIEEKQFVLYYQPKIDVTSGAIVGVEALIRWNEPESGIVLPSRFIPILEETGMI
                     LDVGRWALAEAARVHKKWKAAGVNSGRIAVNVSALQLRQKDFETIVEEATAPVDGEIG
                     IDIEITETMLMQDITHSLSVLGAIRKLGVMIAIDDFGTGYSSLSYLARLPIDYLKIDR
                     SFVSKIADRSDDLEIASAIISMANSLSLKTIAEGVETQEQADLLKALGCHQMQGYLIS
                     KPLPEEKVVEFFL"
     gene            complement(14586..15035)
                     /locus_tag="VE030_10980"
     CDS             complement(14586..15035)
                     /locus_tag="VE030_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZF23973.1"
                     /translation="MTGLEFVEILLVEDNPTDAELTLRALRRNNLANNVVWVKDGAEA
                     LDFLYCRGQYADRKNGTPKLILLDLKLPKIDGIEVLRELKNDSKNKMIPVVMLTSSQE
                     ERDLVESYRLGVNSYIVKPVDFEKFLETVSQVGFYWSLMNKIPPQHS"
     gene            complement(15032..15445)
                     /locus_tag="VE030_10985"
     CDS             complement(15032..15445)
                     /locus_tag="VE030_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23974.1"
                     /translation="MPPAPERVGELLGRHAVNIGFGLALAILTGIAGLQTLAANGLDE
                     AAKLLRLSQRSRLIIPDDVRARIGVGKSVMDQIRELAGDMRNDEEALLRIRSEESRRS
                     DSPSAGDVPWRQGMGRGRRQRGRYLLFFTSRERQR"
     gene            complement(15821..16453)
                     /locus_tag="VE030_10990"
     CDS             complement(15821..16453)
                     /locus_tag="VE030_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZF23975.1"
                     /translation="MLRVLIADDHAITREGLKRILTDHPEAVHVGNAANAAQTLSMVR
                     KNDWDLVLLDISLPDKSGLEVLKSIKKDRPALPVLVLSMYPVDQYALRVLKAGGSGYL
                     TKESAPEQLLAAVRKVSEGGRFITPELAEHMARELAGGTPALPHESLSDREFEVLRLI
                     ASGRTPTEIAGHLSLSVKTISTYRTRLLHKMGMRHNAELTHYAISNRLVF"
     gene            complement(16455..>18440)
                     /locus_tag="VE030_10995"
     CDS             complement(16455..>18440)
                     /locus_tag="VE030_10995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZF23976.1"
                     /translation="WFWHGFVSDFLPQMVWTAGPDGSVDFCNRHWTEYSGLLREDSRG
                     NGWMKALHPDDLENTRRWWDVAVSGGPCHDVRYRLLRGADGDYRWHLGRAAAVRDRAG
                     AILKWIVTAIDIHEQVLGEERLRASEARYRNLVELSRDAIYIRTEGRFVSANAATARL
                     FGVASVDELIGQPVVPFIHPDYLDIVQERMRKLDEEHCELPPLELKLVRRDGSVIESE
                     SIASPCEYQGRSSSHVVIRDITERKALERRQAKLLQELSAYANAAAAAEHFRMLAEAV
                     PSMVWTARADGYLDYVNRPTLDYLGCAFRDIEGWNWDKVVQPEDLPACQARWLHSLAT
                     GEGYEMSLRILRGSDSAYRWYLMRALPVRDAAGAIMLWVGAGTDIDEQKQLETLLQES
                     RTQLEAAVELRTRDLVSVNNALLNEIAERRLTEKALRASREKLRKLSMHLQSARESER
                     AAVAREIHDELGATLTAVKMDLHWYAKTLAKGKPLSNDKLTDTGVLVDSAIQTVKRIA
                     TELRPSILDHLGLWPAIEWQIQELQKHYRIGCELNFESPLVTLEAEAQTALFRIVQEA
                     LTNAVRHARATQIRVHVRKSGDDVVVDIHDNGVGLPPAKLLDPASSGIHGMRERARYF
                     NGEVRFLAGHKAGTCVSARFPLTKKASKPAVRARESI"
BASE COUNT         3640 a         5511 c         5630 g         3659 t
ORIGIN      
        1 ttctcccgga gatcgagctt tctagaattt ggcgttgtcc cggtgttcgg cgcggtcgaa
       61 gcccttcgcg gcatccagcc cgcgcacgcc tttgaatatt gtcccgtcaa cattcgcttc
      121 ctccagattc gctcccgtca catccgcacc ggtcaggtcc gcaccggaga ggtcggctcg
      181 atatagatcg gccccgcgca ggttggcatc cttaaggttg gcaaatcgca gcagcgtccg
      241 gttcaggtcg gcttcggcaa gattggctcc cccgaggttg gccttgacga ggttggtgcg
      301 catctgtccc atcccttgat tcttgatatc aacgccgagg cgcgccttgg acagattggc
      361 ttccgacaga tcggcaccat ccagctctgc gatgatgcgc gctccggaca ggttcgcgtt
      421 cctgaaattc ggacgggcgg tgacttctcc cccgagtatc acgaccgaca tcaacgttgc
      481 gcgggagaga tcggcacccg aaaagtccgt tcccatcagc caggccccgt tcaggttggc
      541 tccagagagc ttcgccccgg aaaaattgca ctgcacgagt ttgctggcga agaaattggt
      601 cccgacgagg ttcacacctc ggaaatcctg tcgccccagg tccaggcggg aaaggtcttt
      661 accggcaagt gcagcggtgg tcacacgctc cggtgacgac aggacctgtt tcacctgttc
      721 gaccgtcaga tctgcggcat aaaccggccg cagcgacgct gcgagcgccg caagggcgaa
      781 acaggcatac atccggctca tggcgcaatc tcccctgaat tcgtattcgg aatcagaagg
      841 actcggcgcc ttcgagttgg gagaacctga ccggaagaat ctccctggtc gacaaccggc
      901 cgttcgcgct cttctccacc aggatcagtt gctggttgtc ggacaagcct gccgggatca
      961 ccattctgcc cccgggcttg agttggttga tcagcggggg agggatgagg tccggcgccg
     1021 ccgtgaccat caccttgtcg aacggagcgt gctcggggtg cccataactg ccgttagcga
     1081 ccttgacctc aacgttggtg tagccggcgc ggctgagccg tttcacggcc tgctgcgcga
     1141 gcgcctcgat gatctccatg ctgtacacct tgtgcgccag ttcggcgagg accgccgcct
     1201 ggtagccaag tccagtgccg atctccagaa cgacgtcttc cttgcggatc tcgagcatat
     1261 ccgtcatcag cgcgacgata aacggctggg atatggtctt gtcgaagccg atcggaagcg
     1321 ggcggttgag ataagcgtaa ggctggatct cgacgggaac aaattcgtgc cgcggcacct
     1381 tgcccatgac ttccatcacc cgcgcatcga ggttgtactt gccgatcctt tcacccgcaa
     1441 ggacggtttg cgcggcaatt tccaggacca tgtgttgccg caggactgcg gcttgctgtt
     1501 cgttcatgcg actgccccat gggctgctcc tgatacagac gatacccctt cggaaggcag
     1561 tcggacaacc tttacggtgg gatcaagagc ccctcatccg atgggattac tgacgctctc
     1621 gattttccgc ttcaccgcca tggggtcgtc gaccgacttg aacacgacat tgctcaagcc
     1681 ccggccggtc acagtcacgt cgccgaaacc gaagatgcgt cccggaatac cctgatcgat
     1741 ctccacggtt tcgatggagt tcagcttcat ctcctcggat ttgcggctga tgatgccctt
     1801 cttgtagatc acgcgcttgt tggtaacgcc ctgctcgatg gaccggagcc tgaggaattc
     1861 aaacagggcc aggatcagcg taattccaac cgtgggaatc gccagcacga tccacaggac
     1921 catccagcgc ctggaaatcc agtggagcct gaaaaatcct acgatctgct cgcctgcgga
     1981 gagcgattct tcaatgtatg gcatggtttg accgtcccgc gctcgccgtg ctataaagta
     2041 cttcaaatat aaacgcttac gggcaggtgt gccagtcatt gaagcccgca tttctgatcc
     2101 cggaggagtt tccctgatgc ccgcagtatc ccgccgagcg aacgatctcg gtaccgaaaa
     2161 cgctttcgtt gtcctggccg aggtcagtgc gttgcaacgc caggggaagg acatcatttc
     2221 cttctgcatc ggccagccgg atttccacac cccgaaaaac atccgcgacg cgggcatcag
     2281 ggcaatcaac gagggcaagc acggctacac gccttcggcc ggtatcctgg agctgcgcga
     2341 ggcggcggcg aattacctga accgcacgcg cgctatcaaa gtgagcgcgg acgacatcgt
     2401 tgttggcgcg ggtgcaaaac cgttcatcgc ctattcaatt ctctcggtca ccgactatgg
     2461 cgcgggcgac gaggtggtgt tcccgaaccc gggtttcccg atctacgaat cgcagattcg
     2521 cgcgcatggc gcagtgccgg tgccgatcca tctgcgcgaa tcgcgcaact tcagcttgga
     2581 tccgtccgag ctcgaagcgg agatcacccc gaaaacaaag ctcctgatcc tgaactcgcc
     2641 gcagaacccg accggcggca tcattccgaa gaaggacatg gaagcgatcg ctgcgatcct
     2701 gcgcaggcat ccgcaagtct gggtgttcgc ggacgagatc tattcacagc tctgctacga
     2761 cggcgaattt gtgtcgctgg cccagtttcc ggatatgctc gagcgcacca tcatctcaga
     2821 tggtgcctcg aagacctggg cgatgaccgg ctggcgcctg ggctttaccg cgaacagggc
     2881 gcttgcgccg gtgttcaccc gctgggtgac caacaccgat tcctgcgcct ctcagatcac
     2941 gcagtgggcg gcggtggagg ccatgaacgg tccgcaggac gaggcgaaga aaatgcgcga
     3001 cagctttctg gcacggcgcg atctgatcgt gagattgctg aacaaagtgc ccggcgttag
     3061 ttgccggaac ccgggtggcg ccttttacgc ctggccgaac gttacagcgg catgcgaaat
     3121 gatcggggcc aaggattcgg aggagttccg taaacggctg ctgaacgaag ccggcgttgc
     3181 agtgctggcg gacatccatt tcggtccgcg cgtgcccaac gaaggccagc acatccgctt
     3241 cagctacgcc gcttccaatg aagcaatcga gaagggcgtc gctcgcatgg cggatttcat
     3301 ccgcaaaaac actaggatta aaaaaaccgc gtgaagtgca agggacgcga aggtacgtaa
     3361 aggaaaatca aaaagggagg gtgcggcatg aaaattctca acaggctgct ggccggattt
     3421 cttgcaactt cgatcgcgtt cacgagctta gtgcaagccg cgccgacgct gatcacgacg
     3481 gaacaggtgg ctgtagaaga cgcgcagaag gaccgcgcgc tggtgaatga aaccttcctg
     3541 cggcccgagc tgcaggcaaa gttgcgggaa tccggggtaa gccttcagga cgcgcaggca
     3601 cgcgtcgcgg cactgacgga ccaggaaatc tccgcgctgg cgcgccagat cgagaccgcc
     3661 ccggcgggcg gaacggacgt tctcacattc gtgctggtca tcttcatcgt gctgctgatt
     3721 accgacatca tgggcttcac cacagtcttc ccgttcgtca agcacggcag cggaaaagcc
     3781 aaatagatct tcaacgcgaa ggacgcaaag gtacgcaaag gaaaccggga tacaagataa
     3841 ccaacgggat aggtggttta aaacctttgt ccgacacgac gaaacgtatc gcttcggcca
     3901 ggcctatccc ctgaatttgg attcgttggt atttgctttc ccttcgcgta cgtttgcgtt
     3961 taacgcgctt cgtccttagc ccgcatagta ttgcagcagc catagggcag ccatgccggc
     4021 cacgatggtt ggcatgattt ttttgaagcg ccacgccacc aggatcgcaa tcacgccggc
     4081 ggtcaggcga tgattgtcca tgcccaccgc caagtcaccg cctcgaacca gcaactccgg
     4141 aaacacgatc gcggtcatca ccgccggcgg cacgaagctc agcgcccgct gcgcaagagg
     4201 tggcatcgcc caattcgcca gcagcgaaat gaacgacagg cggatcagga aagtcagcac
     4261 acctgcagcg attaccacca gccagagatt cattttccca gcctccacgc cagcatcccc
     4321 gcgccgatcc cgcagaacgc ggcgagaatc agcccgagct tgtacggcaa tccaaatgcg
     4381 gctaccgcta caatgccgcc aaccagcgcc gcaaccaggt cggcccgcgt cctgagcacc
     4441 ggcaccagca gggcaatgaa cgacagcgcc agggtgaagt caagcgacca ctcgtgcggt
     4501 atttcggtgc caaggaggat gccgaccgcg gtactcgcct gccaagtgcc ccaaagcgat
     4561 acgccggcgc cgaggaaata ccagtgccgg ctcggttcgc cggcattgtc gcggtaatgc
     4621 gcgatcgcga ccgcgaaagc ctcgtcggtg agcaggtagg agagcaccca cttccatgtt
     4681 cgaggcaggt ctttcacgta tggcgccacc gaagcgctgt ataacgcatg ccgaaggttg
     4741 acgatgaagg tggtcaggac gatcaccagt cccgatgcgc cggcaccgat caactgagcg
     4801 gcgatgaact gcgccgagcc ggcgaaaacg atcaacgaaa tcgcctgcgc catcaccacg
     4861 ggaattcccg cgccggtcgc cagcaccccg tagatgacgc cgaagggggc ggcgccgatc
     4921 aacagcgggg aagtgtcctt cagtccggat aggaactgtg cacgggcggt gttcattgtc
     4981 tttgtgatgc tgcgtcgatc gcccgattgt agacaggcgc tcaacgctgc gtccgtcacg
     5041 gtttcgtgca atcgcggccg gaacagaggc tcgcccggcc gatccgcctt tgttgcttgg
     5101 tccgggccgc ggggggcact acgcacacct caacgggata tcgtaggaaa tcccttgtgg
     5161 atcaagcgtt cgattttcgg ttcagagtgt cggtcggcgc acgctcaagc aggatatcct
     5221 gcatgcgggt ttcgatcgcg ggtttgatct tctggtgggg caggatgtag aacttctcag
     5281 ccttgatcgc ttcgtaaacc aggtccgcga cctgttcggc ggaaatgcgc ccggactgca
     5341 ccgcgtggcg caactgcgct tcccgcttca agtcctccgg gttctttttc ttgtcctcgc
     5401 gcagcgctgc gggacggttg cgctcggaat cggaaatgcc ggtggggacg taggccgggc
     5461 agagcaccga agccttcagc tttgagccaa attcgaccag gtcgtggtgc aggcactcgg
     5521 atagcgtgac taccgagtgc ttggacacgc agtaggcgct catccctggc agcgacagca
     5581 gtccggcaac cgatgccgtg ttgaccacgt ggcaatcgac gttctgctcg agcatgcgcg
     5641 gtacgaaaac gcggatgccg tggatcacgc cccatacatt gacgcccagc gtccattccc
     5701 agtccttctc ggtgtgctcc cagaccgtgc cgccgggcgc gacgccggcg ttgttgcaca
     5761 gaatatgcac gccgccgaag gtcttgaacg ctttgtcggc cagcgcctcg acatctttgc
     5821 ttttcgaaac atcggtgcgc actgccagca ctggaattcg ggcctgttcg aagtcggctt
     5881 cggttttctt cagggcgtcg gcctgtatgt cggccagaac gatcttcatg ccctcgcggg
     5941 cgaagcgcag cgccatggcg cggcccaggc cgctggcacc cccggttacg actgcgacac
     6001 ggtcctggaa ttctttcatc ggcgtgattg cagagggcaa aacacaggga ttctagcatg
     6061 cgggttttcg ccgtcccgcg cagcgttggc aggcctcgag cgcggatggt attctcgccg
     6121 ggctcacagg aggtgaggga gaggaattca tgaacgagga gttcatcggc acgatgccgg
     6181 tccaggagcg gcatcgcttc gatgtggtca gtctggaacg ctatctgcgc cagcacatcg
     6241 caggcttctc gggaaatctc gaagtcgaac agttcagggg cggccagtct aatcccacct
     6301 tcaggctgac ggcaggcgga aagcgcttcg ttttgcgccg caagcccccg ggcaagttgc
     6361 tgccgtcggc acacgccgta gaccgagagt accgcgtcat caccgcgctg cacggcagcg
     6421 acgtgccggt ggcgaaaacc tactgcctgt gcgaggacga cgcggtgatc ggcacggcct
     6481 tctacatcat ggactacatc gaagggcgcg tattgtggga tccatcgctt cccggcatga
     6541 gcacggttca gcgcgcggcg tactttgacg agttgaaccg tgtcatcgcc gcgcttcaca
     6601 aggtggatta cgcgcgagtc ggcctcgccg actacggcaa gccgggcaac tatatcgcgc
     6661 gccagatcaa ccgttggacc aagcagtacc gcgcctccga aaccgagcgc atcgaggcga
     6721 tggagaatct gatcgagtgg ctgccgaaca acatcccgcc gggtgaagag accaccatcg
     6781 tccatggcga ttaccggatg gacaacgtga tttttcatcc gcaggaggcg aagattctcg
     6841 cggtgctcga ctgggaactg tccacgctcg gccatccgct ggcggacttc gcgtatcact
     6901 gcatgacctg gcgactgacg ccgggccagt tccgcggcat gaacgggatc gactttcgcg
     6961 cgctggccat accgacagag caagaatatg tcgccgcgta ttgcaggcgc accggcagga
     7021 gccacatccc gaactgggat ttctacctgg cctacaacat gttccggctg gccgggatac
     7081 ttcagggcat catgggccgg gtcaaggacg gcaccgcggc cagcgcgtac gcgatggaac
     7141 agggcaagcg tgcccggcca atggccgaag ccggctggcg ccaggtcgaa aaaatcctta
     7201 agagctgatt taaagcctaa acctcaccgc agaggcgcaa aggcgcagag gatacgcaaa
     7261 ggaaaacaaa gcaacatgta atggaaaggc aagaaagaaa tggatagtcg cgacaaaggc
     7321 aattcatcga tggggcgatg atggttccga attcctccca acttttatac cggattcgtt
     7381 tggttttctc tgcgtttcct ttgcgccttt gcgcctctgc ggttaactga tttggagatt
     7441 tatgaaagcg gtattgtgta aagagttggg gctgccggag aagctggttg tcgaggaggt
     7501 gccatcgccg aaggcggagc cggggcaggt cgtagtcagc gtgaaagcat gcggggtcaa
     7561 tttccccgat accttgatca tccagggcaa atatcagttc aagcccgagc tgccgttctc
     7621 gcccggcggc gaagtcgccg gtgtggtcaa ggaagtcggt gacggcgtga cccgggtcaa
     7681 gcgcggtgat cgggtgatcg cgttcaatac gtggggcgga tttgccgagg agatcgcggt
     7741 cgacgcggaa cgtaccatcc ctatgccgct ggcaatggat ttcgtaccgg cttccgcgtt
     7801 tgtgctgacc tacggcacct cttatcacgc cctcaaggat cgcgccgatc tcaaggccgg
     7861 cgaaacgttg ctggtgctgg gggccgcggg cggggtcggg ctagcggcga tacaactggg
     7921 caaggcaatg ggcgcgaagg tcatcgccgc tgcctcgaac gatgccaaac tgcagatctg
     7981 caggcagaac ggcgccgatg aggtgatcaa ctacggcacc gaggacctgc ggacacggat
     8041 caaagccatc accgctggca agggagtgga cgtggtgtac gacccggtcg gcggaccgta
     8101 ctcggagccg gccttgcgcg acatggcgtg gaacggtcgc ttcctggtgg tcggctttgc
     8161 cgccggcgac attcccaagg tgccgctaaa tctgaccctg ctgaagggtt gctccatcgt
     8221 cggggtgttc tggggcgcat tcacgaggaa cgaacccgag cgcaatcgcg aaaacaacta
     8281 tgatctgatg gcgatgtacg cggccgccaa ggtcaggccg cacatccacg ctacttatcc
     8341 gttggaacgc gccgcggatg cgctcaacga agtgctcaac aagcgtgtca ccggcaaggt
     8401 tgtcctggta acgggcaaat aaaccttttc accgcaaaga cgcaaggcgc gaaggaaacg
     8461 caaggtaaat caagagaaac atgaatgggg gaacgaggat aaggtctgga cttatcgttg
     8521 ctgaatggtc cgtacgcttc aatgttccct gctgcaccga tcgtatttcg cccagttaaa
     8581 ttcttctttg cgtcctttgc gtctttgcgg tgaagattga ggtctgagat gaggaagaga
     8641 ccatgaatga ggcagtgatt atcgaagcgg tgcgcacgcc gttcggcagg cgcggcgggg
     8701 cgctgcgcga acagcgtccc gatacgttgc tggcgcatgc gctcgacgga ctcatcaagc
     8761 ggtcgcagat cgatccgcag aagatcgaag acgtgatcga cggtacggtc acgctcgccg
     8821 gcgaacaggg cgccaatccc gcccggctcg cggtgctgct ctccgggttg ccggtaaaag
     8881 tgccgggcgt ttcgttgaat cgcatgtgcg gctcgagcca gcaagcggtg cattttgcgg
     8941 cgcaggccgt ggcggccggc gacatgaatt acgtgatcgg ttgcggcgtg gaaagcatga
     9001 cccgtacgcc gatgtttctc gacatcggcc tgggccggac cgtcagcggc ttcgaagtgt
     9061 taaatccgga actgttccag cgccacgaac tgatccatca aggcgaaagt gccgaacgca
     9121 tctccgagaa atggggcttg tcgcggcgga tgctcgacga tttcgccagg gaaagccacc
     9181 ggcgcgccgc gaaggctcag aaggccggct ccaacaagga gctgttgccg gtgcagggcg
     9241 tatcgcccga aggcgaggcg atgcggctgg agcgcgatga aggcgtgagg gcgcagattg
     9301 acgaggccaa gatggcttcg ttgaagccgg cgttccgccc ggacggaaac ggcgtgatta
     9361 ccgcggcgaa ttccagccag atctccgatg gtgccgccgc cttactggtt gcgaacaggg
     9421 acgtcgcaaa ggccgacggt ttcaaaccga gagcgcgctt ccgtgcccgc atcgcggttg
     9481 gttccgatcc gatgatgcaa ctcaccggcg tcattccggc gaccgaactg gcgctgaaga
     9541 aagcgggagt ctccatcaag aacatcgact ggatcgagat caacgaagca ttcgccgcgg
     9601 tggtgctgtc ctgggcaaaa gaaatccatc ccgacatgga caaggtcaat ccctggggcg
     9661 gagccatcgc ccacggacat ccgctgggcg cgacaggcgc cgggctgatg tccaagttgc
     9721 tggccgggct ggaagctacc ggcggcacgc tcggccttca ggtcatgtgc atcggccacg
     9781 gccaggccac cgcgaccatc atcgaacgaa tctgaattgc atctagccct tcaccgcaaa
     9841 gacgcaaaga cgcaaaggga acgcaaggga caacagagtt gaaaatgagg tcgtgcctgt
     9901 tgttcccgga tattggtgtt ggcgatcggc gcccgcgtgt tgacgagttg atcgcctggt
     9961 gaaagccatc gtcgccaaac cattacggcc aagcgttaac cgcaagggac gccaaggacg
    10021 ccagggaaag ctagatgcaa gaatcaggtg agtgatcaaa cccggtgcaa gtatctgcgt
    10081 tggactggcc cgttacccta acccagcctc gcctccacgc tattaccttt ccttggcgtc
    10141 cctggcggtg gatgtgtcat tgttgaggcg ttcgaagccc acggaaaagt ctgaatgacc
    10201 aggaatgaag gctgaaaaat gaaggataaa ggcggcatgc gccgcaatct caccaattac
    10261 ggcgatccgg agttcgcggc ctacttgcgc cgctcctttg cgcgctcgat gggttactcc
    10321 aaggacatgc tggagcggcc gatcgtcggc atcgcccagt ccgcgagcgg attcaacaac
    10381 tgccatcgct acgtgccgga gctcaccgag gcggtcaagc gcggggtact caagagcggc
    10441 gggctgccga tcgacttccc gaccatttct ctgggcgagg tattcctcag ccccaccagc
    10501 ctgatgttcc gcaatctcat ggcgatggac gtggaggaaa tggtgagggc gcagccgatg
    10561 gatgcggtgg tgctgatcgg cggctgcgac aagaccgtgc cggcgcaact gatgggcgcg
    10621 gcctccgccg acgtgccggc aatccagctc gtcaccgggc cgatgatggc aatgccttac
    10681 aggggcgagc gcctgggggc ctgtaccgat tgccgccggt tctgggccag gtttcgcgcc
    10741 ggcgaagtca gcgatgaggg catagacgaa atcgagggca atctcgccac gaccgccggc
    10801 acctgcgccg tgatgggaac ggcgagcacc atggcctgcg tcgccgaggg tttgggtatg
    10861 agccttcccg acacggcggc gattcccgca gtgcacgcgg accgtatacg tgcggcggag
    10921 gccacgggcg tacgcgcagt ggagatgatc aggacgaagg gcccgcggcc ctcggagatc
    10981 atcacggaga aatcggtcga gaacgcaata cgcgtgctgc tcgccgtcgg cggctcgacc
    11041 aacgcgatca tccatctcac tgcggtcgcc ggccgtcgcg gcatcggaat ctcgctcgag
    11101 cgcctgaacc ggctttctga cgaaacaccg gtactgctgg atctcaaacc caccggcagc
    11161 cactacatgc aggatttcca cgcagcgggc ggaatgagcg cgctgctcgc cgaactgaag
    11221 gaccttctgc atctggactg catgacggtc accggcgaga cgctgggtag tcgaatcgcc
    11281 agtggcgtag cgttcgtgga ccgtggggta atccgtcccc ggagcgatcc gatttccccg
    11341 gtcggcgggc tggtggcgct gttcggcagc ctcgctccgc gcggggcgat actcaaacgc
    11401 gccgcggccg attccggtct gttcgagaag gagggcaggg cggtcgtttt cgagtcactc
    11461 gaggatctgt cggcacgaat agacttgccg gacctcgatg tgactaagga tgatttcctg
    11521 gtgctcaaga atgcgggacc gcgcagcgct tccgggatgc cggaggcggg ctatctgccg
    11581 attccgaaga aactggcgaa gaccggactc aaggacatgg tgcggatctc tgacgcgcgc
    11641 atgagcggta ccgcgttcgg cacggtggtg ctgcacgtcg cgcccgatgc ggcttcaggt
    11701 gggccgctgg cgctggtgaa aagcggcgac cgcatccgcc tgtcggtcag gaatcggagg
    11761 ctcgacctga tggttgccga ggccgacctg gtcaagcggc gcgacgggtt caggacgccg
    11821 catctcccgg agcgcggtta cgcccggctt tacaacgagc atatcctgca ggcagacgcc
    11881 ggttgcgact tcgacttcct gcggggatcc tgatccgcgc tcacaagaaa aattcgacca
    11941 ctttctcttc gggcaacggc ttactgatca aataaccctg catttgatgg cagccaagcg
    12001 ccttgagcag atccgcctgc tcctgggttt cgacgccttc ggcaatggtc ttcaagctga
    12061 gggaattggc catggaaatg atggcggatg cgatttccag atcgtcggac cggtcggcga
    12121 tcttgctgac gaatgaccgg tcgatcttga gatagtcgat cggcagcctc gcaaggtagc
    12181 tcagcgaaga atagcctgtg ccgaagtcgt cgatcgcgat catcacgccg agctttcgga
    12241 ttgcgcccag gacagacagg ctgtgcgtga tgtcctgcat caacatggtt tccgtgattt
    12301 cgatatcaat cccgatttcg ccgtccacgg gtgccgtcgc ttcctccact atggtctcga
    12361 agtccttctg gcgcaattga agggctgata cgttgacggc aatgcggccg ctattgaccc
    12421 ctgccgcctt ccatttcttg tggacccggg cagcttccgc gagtgcccac ctgccgacgt
    12481 ccaggatcat tccagtttct tccaggatcg gtatgaaccg cgatggcagg acgattccgg
    12541 attccggttc gttccagcgt atcagcgcct cgactcccac aattgcgccg cttgtgacgt
    12601 cgattttcgg ttggtaatac aagacgaact gcttttcctc gatcgctcgc cggagcttgg
    12661 tttccagtgc cagacgctgc gcgaaatcgg cgttgagggt cggtgtgtag tagacggtcg
    12721 cgtcgccggt ctgtttggac tgggtcaggg cggcctcggc attgcgtatc agtgtttccg
    12781 aatcctgacc atctgctgga aacactgcga cgccgcacct ggcagacacg cgaatatcgt
    12841 tcccgtccag atcgaagggc cgcgagaacg gattcttacg gccctcttcc accagcctcg
    12901 cggcgtggat tccatcccgt atgtcaggca atacggcaat gaaactgttc atgccgatgc
    12961 gcccgacctg ctccggcaca tgctggctct gaagccgttc ggcgaccagc ttcagcaatt
    13021 tgtctcccgc gcctctgccg agcgattcgt tgacgatgtg catgcgctcc aggtcgagaa
    13081 tcatgagcgc cagcatgtgc cggttgcgcg tggcctcgtg aagaaactgt tgcagccttt
    13141 cctgaaccag cgtgcggttt gcgagccccg tcagcggatc gtagaatgac aggtactcga
    13201 ccagatcctg tttttccata cgttcgagcg ccagcgccgc atccgccgcg agatcgctga
    13261 gcagtctgag atgctcaaca tcgaagaagt gcggctcctc ggaaaacagg acgccgacgc
    13321 ccgctacctt gtcgccggaa ttcagaggga gcgcggcgca ggaacggaag ccgcgtacaa
    13381 gggccgcctg cttccagtca ggatcggtgt ccgaggtctc gatatcgtca attacgatct
    13441 gccgttgtgc gcgcaccgcc atggttgccg ggatttcctg ttccaggcgc acgatgtcgc
    13501 gaccgccggt aacgacctcc agaaaaccgt ccaccgcacc atgcgatgcg actggacgaa
    13561 ttccctccgc gtccttttgt accaggcccg cccacgccat cctgaagtgt cccacatcga
    13621 tcgcaatccg gcacaactcc cggcatagat cctccggagt acgcagcctg ataattgcgg
    13681 ctgtcaggct gctgagcacc tcgcggatgc gcgacaggcg ctccagtttc cgttcctgaa
    13741 ggatgcgatc ggtgatgtcg accgcaacac cgccgatcat ggcgacattg tcggattcgt
    13801 ccttgatcgg aaacttgctg accaggaaga agcggtcgct ctcgccgaat atcactttct
    13861 ccacgacccg gtaaggttgc cggctttcga cgaccttgcg atcgttggcg acgagcggct
    13921 caacgtagtc cggcgggtaa agatcgtgtg aagtcaggcc gatcaattgt ccgtccggtt
    13981 ttcccgcaag ctcttcgaac gtcgggttgg cgaacgtgaa gcgtccttgg ggatccttcg
    14041 taaaggccgc cgcaggcaaa tggcgcatga aaagcgagaa gtagtgttcc gtgtccttcg
    14101 cccgccgttc cgccgccagg cgcgccttgc gttcgcgcgc ctccttgatg gcacgaagga
    14161 ctgcgggcgc gaaccgcttc aggttggatt tcagcacgta atcggtggcc ccgttcctga
    14221 gcgctgcgac ggcgttctcc tcgccgattg tgcctgacac gaaaatgaaa gggatgtcgg
    14281 ggcgcagttc gcgtgagatc gaaagcgcct ccatgccgct gaaacgcggc agcgagaaat
    14341 cggacagaat cacatgtgga tcgaagcttt cgatctcttt tctgaagtcc gtttcgattt
    14401 ccactacccg tgccgcaatg tgaagccccg cacgacgcag ttcacgcagt tccagctccg
    14461 catcgacgct gacgtcttcg accatgagga ttcggattgg gtcattctgc ttgtccggct
    14521 ctttcgtcat gtctttatga attgaaaagg ttcgaatcgg tattcgatac acacaacaag
    14581 tgcactcatg aatgctgcgg tggaatcttg ttcatgaggg accaataaaa tcccacctgt
    14641 gaaaccgttt caaggaattt ctcgaagtcc accggcttca cgatgtagct gtttacgccg
    14701 aggcgatagc tttccaccag gtcgcgttcc tcctgcgacg aggtcagcat gaccacgggt
    14761 atcattttat tcttgctgtc gttcttgagc tcgcgcagaa cctcgatgcc gtcgatcttc
    14821 ggcagtttga ggtccagaag aatcagtttc ggggttccat tcttgcggtc cgcatactgt
    14881 cccctgcagt aaaggaagtc gagcgcttcg gcgccgtcct tgacccacac gacgttgttg
    14941 gcaagattgt tacggcgcaa cgcccgtaac gtgagttccg cgtcggtggg attgtcttcc
    15001 accagcagta tttccacgaa ttcaagcccg gtcatctttg tctttccctg gaagtgaaaa
    15061 ataaaaggta gcgccctcgt tgacggcggc ctcggcccat accctgccgc catggcacgt
    15121 caccagccga tggactatcg ctgcgccggg attcttccga gcgaatccgc agaagggctt
    15181 cttcgtcgtt gcgcatgtcg ccggccagct cgcgaatttg gtccatgacc gacttgccca
    15241 cgccaatacg tgcgcgcacg tcgtccggaa tgatcagcct ggatcgttgg ctcagccgca
    15301 agagttttgc tgcttcgtcc agtccgttgg cggccagggt ttggagcccg gcgattcccg
    15361 ttaggatggc caaggcaagc ccgaatccga tatttacggc gtgccggccc agtaattcgc
    15421 cgacgcgctc aggtgccggc ggcatcggcg gcgcacacgg gcgggcgttg ctgacatacc
    15481 ggagagtacg ggatttgttc gcgactatgg tatttgctgt aaagaacgcg accggaccgc
    15541 gggctgcaat cctttgtgtg cgaaacggct gccggcatga cgaacggggc gcaccgcctc
    15601 gccttcaagc ccggtgtagg gcacaggcga aaccgagtta ggttcatgca ctgttacatg
    15661 ctgccaggtt tggactcctt tatttttatg tgcaaaatta ccggcgcgtg cagctattct
    15721 gacagcattg gctctgcagt gccggagagc aatcggggag ggcctgatat ttgtgtagcg
    15781 atttgtccta caagtcgaaa tagctcttat ggagagacgt tcagaaaacc aggcggttgg
    15841 agatcgcgta gtgggtgagt tcagcgttgt ggcgcatgcc catcttgtgc agcaggcgcg
    15901 tgcggtaagt gctgatggtc ttgacgctca acgacaggtg tccggcgatt tccgtggggg
    15961 tcctgcccga tgcgatcagc ctcagcacct cgaactcgcg atccgacagg ctttcgtgcg
    16021 gcagcgcggg cgttcctccc gccagttccc tggccatgtg ctcggccaat tcgggggtga
    16081 tgaatctgcc gccttcggat accttgcgga ccgccgccag taattgctcg ggcgcgcttt
    16141 ccttggtgag ataccctgag ccgccggcct tgagcacgcg tagcgcgtac tgatccacgg
    16201 ggtacatgct gagtacgaga acgggcagcg ccggccggtc ctttttgatg ctcttgagca
    16261 cttccagccc gctcttgtcc ggcagcgaga tgtccagcag caccaagtcc caatcgtttt
    16321 tgcgcaccat ggaaagggtt tgtgcggcgt ttgcggcgtt gccgacatgt accgcttccg
    16381 ggtggtccgt caggatgcgc ttgagccctt cgcgcgtgat ggcatgatcg tcggcaatga
    16441 gcacgcgcag catttcagat cgactccctg gccctgacgg cgggctttga tgctttcttc
    16501 gtcagcggga agcgggcgct cacgcaggtg cccgctttgt gcccggcaag gaaccgaacc
    16561 tcaccgttga agtagcgggc gcgttcgcgc attccatgaa ttccggatga agccggatcc
    16621 agcagcttgg ccgggggcag accgacaccg ttatcgtgga tgtccaccac gacgtcatcc
    16681 ccggacttgc gcacgtgcac gcgaatctgc gtggcgcggg cgtgcctcac cgcgttcgtg
    16741 agcgcctcct ggacgatgcg gaacagcgcg gtctgcgcct ctgcttcaag agtaacgagg
    16801 ggtgactcga aattcaactc gcagccgatg cgatagtgct tctgcagttc ctggatttgc
    16861 cattcgatgg caggccagag gccgaggtgg tcgaggatgc tggggcgcag ctcggtggca
    16921 atgcgcttga cggtctgtat agcggaatcg accagcacac ccgtgtcggt gagcttgtcg
    16981 ttggaaaggg gtttgccctt ggccagcgtc ttggcgtacc agtgcagatc catcttcacc
    17041 gcggtgagcg ttgcccccag ttcgtcatgg atttcgcgcg ctaccgcggc gcgttccgat
    17101 tcgcgcgccg attgcaggtg cattgagagc ttgcgcagct tttcccgcga ggcacgcaac
    17161 gctttttcgg tcagtctgcg ttcggcgatc tcgttgagca gggcgttatt gacggatacc
    17221 agatcccggg ttctcagctc taccgcggcc tctagctgcg tgcgcgactc ctgcaggagc
    17281 gtttccagtt gtttctgctc gtctatgtcg gttcccgctc ccacccagag catgatcgca
    17341 ccggcggcat cgcgcaccgg cagcgcgcgc atcagatacc accggtacgc cgaatcggaa
    17401 ccgcgcagga tacgcaggct catctcgtag ccttctccgg tcgccaggct gtgaagccag
    17461 cgtgcttggc acgccggcaa atcctccggt tgcacgacct tgtcccagtt ccaaccctcg
    17521 atgtccctga acgcgcatcc caaataatcg agcgtgggac gattgacgta atccaggtaa
    17581 ccgtccgcac gcgcagtcca gaccatgctg ggaacggctt ccgccagcat ccgaaaatgc
    17641 tccgcggcgg cggctgcgtt cgcgtacgcg gacagttcct gcagcagctt ggcctgccgc
    17701 cgctcgagcg ccttgcgctc ggtaatgtcg cggatcacga cgtgtgacga tgagcgtccc
    17761 tggtactcgc acggcgaagc gatggattca ctctcgatca ccgatccgtc tctgcgcacc
    17821 agcttcaatt ccagcggcgg cagttcacag tgttcctcgt cgagcttgcg catgcgctcc
    17881 tgcacgatat cgaggtaatc cggatgaatg aaaggtacga ccggctggcc gatgagctca
    17941 tcgacgctcg ctacaccgaa aaggcgcgcg gtggcggcgt tggcggacac gaacctgccc
    18001 tcggtgcgaa tgtagatcgc gtcacgggag agttcgacca ggttgcggta acgcgcttca
    18061 ctcgcccgaa gccgttcctc gcccaggacc tgctcgtgaa tgtcgatcgc cgtgacgatc
    18121 cacttcagga tggcacctgc acgatcgcgc accgctgccg cgcgccctag atgccagcga
    18181 tagtctccgt cggccccgcg caaaaggcga taacgaacgt cgtggcaagg cccgccgctt
    18241 acggcaacgt cccaccatcg ccgcgtgttt tccaggtcgt ccggatgcag ggctttcatc
    18301 catccgttgc cgcggctgtc ttcgcgcaac aggcccgaat attcggtcca gtggcggttg
    18361 cagaaatcga ccgacccgtc gggccccgcg gtccaaacca tctgcggcag gaaatcggaa
    18421 acgaatccgt gccagaacca
//
LOCUS       DASQOP010000279         3698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1552380, whole genome shotgun sequence.
ACCESSION   DASQOP010000279 DASQOP010000000
VERSION     DASQOP010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552380"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..1702
                     /locus_tag="VE030_11000"
     CDS             53..1702
                     /locus_tag="VE030_11000"
                     /inference="COORDINATES: protein motif:HMM:NF015078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23977.1"
                     /translation="MTATTASGFPAAVLRWIDRNILALGREMRLSYMPPLMVYAAAGI
                     SGLTGIVGAFYVKEQLGLSAEFLAALGFWMMLPWALKMPLGHLVDLVWRWKSLLVYLG
                     AGLITLSILIMIGLLGHTDAMRTVAPVETWFVLAALLAPIGYVLQDVVADAMTVEAVP
                     RFGSDGQRIGAAERKPMHTTMQTLGRVAIIGGSVAVSILNIFLLRGVGALPEAQKAAA
                     YLSVYQLALIIPLVSVLGVVLASWLRRRGLARLAAEGRTRAESEAILDRHFDPPPVNW
                     WILGGGLVFAVVSLSVGLGQIPAAQEIVFCASMAIVIFLMWRLTRELEPEARNFLVGT
                     AIVVFAFRAIPSPGAGSTWWAIDALGFDQQFMATLGLIGSALTLFSLFIFRRFMAEHS
                     IAYVVGVLTIIGTLLSLPNIGMYYGLHEWTAAHTNGVVDARFIALIDTALESPLGQIA
                     MIPMLAWIANSAPEKLKATFFAVMASFTNLALSASQLGTKYLNRFFSVSREVRDPVTG
                     QVVAPADYSELGALMITAAVIAFALPFLAILFSRLTRFRNA"
     gene            1793..2257
                     /locus_tag="VE030_11005"
     CDS             1793..2257
                     /locus_tag="VE030_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HZF23978.1"
                     /translation="MMETHVTDIARIIQLAVAPVFLLTAIGTILSALNNRLGRIVDRR
                     RVLEDRLRTPPGEETGRVKEDVEELNLLAQRIRLIYHAIMLAVVCALFVCLLVAGAFL
                     GVFITADLAKVVGTLFILAMFALVGSLGMFLREIFLAVKGGRHQLTLNDVIK"
     gene            2637..3140
                     /locus_tag="VE030_11010"
     CDS             2637..3140
                     /locus_tag="VE030_11010"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZF23979.1"
                     /translation="MSDRKTRNIALVPALPADALAVAEMSRDLIETGLGWSWSRQRVA
                     RAISDAETLVLTARHRGRVVGFGIMEFGEEKAHLSLLAVKPGYQREGIGTQVFGWLKE
                     SALTAGIAIVKLELRATNVVAQQFYRSLGFEEAGWAVGYYRGQETALRMALHLRLQSA
                     GRSHQAL"
BASE COUNT          684 a         1077 c         1201 g          736 t
ORIGIN      
        1 ccggcgggaa cgcacaacaa gacggcctgg ttgtttcagg atcacccaca gcatgactgc
       61 cactactgca tccggttttc ctgccgcggt actgcgctgg atagaccgca acatcctcgc
      121 gctcggacgc gagatgcggc tttcctatat gccgccgctg atggtatatg ccgctgccgg
      181 catctccggc cttaccggca tcgtcggcgc cttctacgtg aaggaacaac tcgggttgtc
      241 cgccgagttc ctcgccgcgc tcggtttctg gatgatgctg ccatgggcgc tgaagatgcc
      301 gctcgggcat ctggtggatc tggtctggcg gtggaagagc ctgctggtgt acctcggtgc
      361 gggactgatc acgctcagca tcctgatcat gatcggtctg ctgggccaca ccgatgcgat
      421 gcggacggtc gcgccggtcg agacctggtt cgtactggcg gcactgctcg cgccgatcgg
      481 ttacgtgctg caggatgttg tggccgatgc gatgaccgtc gaggccgtgc cgcgcttcgg
      541 cagcgacggc cagcgcatcg gtgctgcgga gcgcaagccg atgcacacga ccatgcagac
      601 gctggggcgg gtcgcgatca tcggcggcag cgtggccgtg tccatcctga acattttcct
      661 gctgcggggc gtcggtgcgc tgccggaagc gcagaaggcc gctgcgtatc tttccgtcta
      721 ccagcttgcc ctgatcatcc cgctggtctc cgtactggga gtggtgctgg catcgtggct
      781 gcggcggcgc ggcctggcgc ggctggccgc agaagggcgc acgcgcgcgg aatccgaggc
      841 gatcctcgac aggcatttcg atccgccgcc ggtgaactgg tggatactgg gcggcgggct
      901 ggtgttcgcg gtggtttcgc tgagcgtcgg actgggccag ataccggccg cgcaggaaat
      961 cgtattttgc gcgtccatgg ccatcgtcat tttcctgatg tggcggctga cgcgcgagct
     1021 cgagcccgag gcgcgcaatt ttctggtggg aaccgcgatc gtggtgttcg cattccgcgc
     1081 cataccgagt ccgggtgccg gctcgacctg gtgggcgatc gacgcgctgg gtttcgacca
     1141 gcagttcatg gccacgctgg ggttgatcgg atcggccctg acgctgttca gcctgttcat
     1201 cttccgccgt ttcatggccg agcattcgat cgcctacgtc gtcggtgtgc tcaccatcat
     1261 cggaacgctg ctgtcgctac ccaacatcgg catgtattac gggcttcatg agtggaccgc
     1321 ggcacatacc aacggtgtgg tggacgcgcg cttcatcgcg ctgatcgaca ccgcattgga
     1381 gtcgccgctg gggcagattg cgatgatccc gatgctggcc tggattgcca actcggcccc
     1441 ggaaaaactc aaggccacgt tctttgctgt gatggcctcg ttcaccaacc tggcgctgtc
     1501 ggcttcgcaa ctcggcacca aatacctgaa caggtttttt tcggtatctc gcgaagtgag
     1561 agatcccgtc accggacagg tcgtcgcgcc ggccgactac agcgaactgg gcgcgctgat
     1621 gatcacggcg gcggtgatcg ccttcgcgct gccgtttctg gccatcctgt tctcgagatt
     1681 gacgcgtttc cgcaatgcct gagcacgatg aaatggtcgg actcgcacgc cggcacggga
     1741 aagccgtgtt tcaccgcttc gaagaggttc ccggctgcgc cggaatggag gaatgatgga
     1801 aacccatgtc accgatatcg cccgcatcat ccagctcgcc gtcgcgccgg tgttcctgct
     1861 gaccgcgatc ggaaccatcc tttcggcgtt gaacaacagg ctcggccgca tcgtcgaccg
     1921 ccgccgcgtt ctcgaggaca ggctgcgcac gcccccgggc gaggagacgg gccgggtcaa
     1981 ggaggatgtc gaagaactga acctgctggc ccagcgcatc aggctgatct atcacgccat
     2041 catgctcgcc gtcgtatgcg ccctgtttgt ctgcctgttg gtcgctggag cgtttctcgg
     2101 ggtcttcatt accgccgacc tcgccaaggt ggtcggaacg ctgttcatcc tggcgatgtt
     2161 cgccctggtc ggatccctgg ggatgtttct cagagagata ttcctcgccg tgaagggtgg
     2221 acgccaccaa ctcaccttga acgacgtcat caagtgaata gggcaacagc gttgacgcaa
     2281 agaacgcaaa ggggaaatga aaggacgcaa aggaatactt gggcgaggat caagcaacga
     2341 gtggctggtg gttaggggcg agaggcgaga ggttcgacaa gaatctcgtg catttatttt
     2401 tctcttcaca tattctttct ttgcgttcct ttgcgtcatt tgcgtctctg cgttgaaggg
     2461 cttgaacttg aatttactag cggcaaaggg cactcccgag gcgaagaagt ccggagaggt
     2521 cgttacgccg ggcgccggca aggcattcta cgttggccgc ttaggcaggg tcgggtcgta
     2581 atctgcgctt aggcgcgcgg caaatacaat tccgaataag cagaaacgat gctcagatga
     2641 gcgacagaaa aacccgcaat attgccctcg tgcccgccct tccggcagac gccctggcgg
     2701 tcgccgaaat gtcgcgcgac ctgatcgaga ccgggctcgg atggtcctgg agccggcaac
     2761 gcgtggcgcg ggcgatcagc gacgcggaaa cgctggtgct gaccgcccgc catcgcggcc
     2821 gggtggtcgg gttcggcatc atggagttcg gcgaggaaaa ggcccacctc tcgctgttgg
     2881 cggtgaaacc cggttatcag cgcgagggca tcggcacaca ggtgttcggg tggctgaagg
     2941 aatcggcgct gacggcgggc atcgccatcg tcaagctgga attgcgcgcc accaacgtgg
     3001 tcgcccagca gttctaccgg tccctcggtt tcgaagaagc cggatgggcg gtcggttact
     3061 accgcgggca ggaaaccgca ttgcggatgg cattgcacct gcgcctgcaa tcggccggac
     3121 gttcgcacca ggcgctctga ggcggcgaag gccataagaa atagagcaca gagttgcatc
     3181 acttgacgaa aaggcaactg catcggatga ttcacggcag cgaattaaag gggagcattc
     3241 ccatgccgat gcaacggata gtgatcatgt tgggggcgtt ggccggcgta cttttaaatt
     3301 tgaccgcggc cacggctgat gaacagacgc tgccagatgc cgcggaccag aagattcaga
     3361 caagcatact gtttgtcgat tcgcacgacg caataggcaa atttgtgaaa gttcggaggg
     3421 acggggtccg tcataggcgc tcgagggctc atgcggatgc gcgaatctgc ctcgacgctg
     3481 ccaacagagc cgccataatt cgctgcgcgg agaagtaccg ctaacgctca ccgcagagga
     3541 cgcggaggac gcggaggacg ctgagagatt acctgttatc ccattgtccg aggcagccac
     3601 agcgagatcg cgggaaaggc gcaaagcacc agcagccgga ccaggtccgt ggcgatgaac
     3661 ggcatggtgc cggcgtagat gcgcggcaag ctgatgtc
//
LOCUS       DASQOP010000280        55008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_741091, whole genome shotgun sequence.
ACCESSION   DASQOP010000280 DASQOP010000000
VERSION     DASQOP010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 55008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55008
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741091"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="VE030_11015"
     CDS             <1..1047
                     /locus_tag="VE030_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23980.1"
                     /translation="MRLNRKGILTRAGLAWWRESVTQVLRKADRYAGVYVVRLGIEAA
                     KRDHEERVKLMGGNAQPLDLSGVTEARLDLPVIIDAETARLARAIQTQNKTKRAGRPT
                     KYFPLSKLVYCSVPNGNGPCGGVWYFRNNGEARHGIGYCSKHNYQGGGIRLKCHASEM
                     VAAKLEAIVIDAMKEHLRQPEVAHAAAIQAYRAEHGVDAHQRRADSEAALEALREQQE
                     HYGTIILNPAMKKLHGKANTQFAALEIQIQALERELRHAPVSMMYSEASIVAAFGAML
                     GDLEEVQTFEEKREFLQSTVERIEMTGEEVVIRGAISIPQDAVSGGQNWNGCGPSDSN
                     FTPSIPFVIKRRVA"
     gene            1044..1301
                     /locus_tag="VE030_11020"
     CDS             1044..1301
                     /locus_tag="VE030_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23981.1"
                     /translation="MTREPTLTTFCAACAACIAGRRHAPEEVQAHHPFSGHGFSPEVG
                     WTDPRLEPKPTTLHSWPMEYDPTPFAPGETAVVPSTKGRVA"
     gene            1528..1809
                     /locus_tag="VE030_11025"
     CDS             1528..1809
                     /locus_tag="VE030_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23982.1"
                     /translation="MRVAYRDNPAIAGTVNLIRSWERGEIAEPTMSTSSGPKATIRNH
                     IFAIFERYPHVGRVLMVIGKYSQSVPFLLVVDEAGQFFDMAGKEVVVDG"
     gene            1820..2005
                     /locus_tag="VE030_11030"
     CDS             1820..2005
                     /locus_tag="VE030_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23983.1"
                     /translation="MTVAYLGWHRSEQNLTICANDGRAIVRITRDGKVIVNPDFTIDE
                     AARAFWEAVQKLAAKEK"
     gene            2006..2383
                     /locus_tag="VE030_11035"
     CDS             2006..2383
                     /locus_tag="VE030_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23984.1"
                     /translation="MAEKRLYEAIKYTFGPTEMRELGESLAHENQNCFNLEDQKANTV
                     AALAAAIKSAKSRVVELTTKINNGYEMREVECLAILETPRPGMKRIIRLDTNEMVREE
                     AMTVNEMQSGFGFIAPGPEAPGE"
     gene            2380..2592
                     /locus_tag="VE030_11040"
     CDS             2380..2592
                     /locus_tag="VE030_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23985.1"
                     /translation="MNPALGYGVCPDCGAVSHGNLTCRTCRQHMRQTAGCPADFLDQL
                     DAANIATVAPVGPAAIRAFLKRLEGE"
     gene            2592..2954
                     /locus_tag="VE030_11045"
     CDS             2592..2954
                     /locus_tag="VE030_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23986.1"
                     /translation="MAKRPFRLTPPPISEDDIEAGCKTVLALHSYLVIRLHAGVYKTL
                     DGARHVHGVPKGTPDYACLHEFHRNFLLEVKRPGGKLNPDQETQIAAIRQQYRLPVVV
                     AEGVEEFCNWLARHEHPP"
     gene            3572..4429
                     /locus_tag="VE030_11050"
     CDS             3572..4429
                     /locus_tag="VE030_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23987.1"
                     /translation="MSASLIVQHIKTYQDDDRVRDTDLAEWLGYADPRKIRQTINRHR
                     TQLEKVGVIHTVGITSGDLGGRPASENWLTVKQCLLLCTYAETANADQVRIALVEAFD
                     QYRKSKLVAVEPSLEMSVLDTQARQIWVPLQRMVRQEIEPMQNDVRALRGDVQEVKQI
                     VRTEADDIKREIRESRQKYRWKPIDQADKLLHIDCLWRRHAGICPRCKRTVIVDQDGV
                     PTAEFEIDHNFAPWKNALTETWPLCSRCNKEVNAAEERQKCQDDFHSYQKSLQAHRED
                     LHFPLLRHL"
     gene            4426..4836
                     /locus_tag="VE030_11055"
     CDS             4426..4836
                     /locus_tag="VE030_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23988.1"
                     /translation="MKALTPETAAAVVGKLALMAFFPSDPEVRAGLVAIFIEMVETDE
                     QMEWLGSRAMRMFARWPGVAEIRALYCSRYKPRDGLESYSEIYPEGFPTERKEPEPGR
                     AARRGEPVTADPVLDRKIRTVAASKKLKPVRKGR"
     gene            4866..5468
                     /locus_tag="VE030_11060"
     CDS             4866..5468
                     /locus_tag="VE030_11060"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HZF23989.1"
                     /translation="MERWPLARLIPYARNARTHTPDQVKQIAASMREWGWTIPVLADE
                     AGGIIAGHARVMAAELNQYAEAPVMIARGWSDAKKRAYVLADNKLAMNAGWNEELLGV
                     ELLELRDAGFDMPVIGFDASELDGLMREVAEAGYPVLPEGDRAPYQQITFLLHDEQVK
                     IVQAAVTAAKQRGPFVGTHNDNVNGNALARICAAYLEEEN"
     gene            5470..5727
                     /locus_tag="VE030_11065"
     CDS             5470..5727
                     /locus_tag="VE030_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23990.1"
                     /translation="MQSPSSYDPDLRSARRSALTSGVLNLAVVGFHVYRHSWAVVAAS
                     AVWTLSSWIWLVTIRAQQTTRDYSRVVEAATRREIERREFR"
     gene            5724..6092
                     /locus_tag="VE030_11070"
     CDS             5724..6092
                     /locus_tag="VE030_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23991.1"
                     /translation="MTDRLIWAELFPGQPWPGEEAVLRALAAEVRTLRSTIVNEPQLR
                     RTLDEIQRAHDLVGQYCYETQDEATFQFVSALCWVLGHDHNPAFADTLATIETLLEGL
                     GVQVVRLPHLVYPTRGESVK"
     gene            6203..6829
                     /locus_tag="VE030_11075"
     CDS             6203..6829
                     /locus_tag="VE030_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23992.1"
                     /translation="MSANSCIAAARTTIETMRLYRLRAVEKPVCYVFQVPARNYARLA
                     GFSAEERAEMKAKAGNWVDATPDGGSWNGHLIVVVEDRWILDPSIDQADAPQFGVSVP
                     AEVFVADTLGQTWHPNMNFEIELGLILDNGDAASLLYHSISDRGYLETEAWTDEGLPL
                     LAHAIASEMKNAAAVSIACPKCGRVSHNANDVRERYCGNCHSFHDDAR"
     gene            6819..7010
                     /locus_tag="VE030_11080"
     CDS             6819..7010
                     /locus_tag="VE030_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23993.1"
                     /translation="MRAEILDRAVAFEVQPEARPDGSWEPPAEVTLTLRPCWRKPPVR
                     LRVAYLYTYEQVAYYAEEL"
     gene            7007..7186
                     /locus_tag="VE030_11085"
     CDS             7007..7186
                     /locus_tag="VE030_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23994.1"
                     /translation="MIRCAICQDDGWIAYPTFADAEKQVIRIRKVACPRGCPLANPTE
                     EGVLATFPSDQEGEA"
     gene            7183..7512
                     /locus_tag="VE030_11090"
     CDS             7183..7512
                     /locus_tag="VE030_11090"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZF23995.1"
                     /translation="MSTKEFCKQSRLTPRKVQWWCETGVLRCSQRERGFRDFDEAELR
                     VAALVAQLRRKGVALTRIRQMRIRNPQGDFLVANGAAYWWCTREELLPELAKTRGACV
                     VVSLKGL"
     gene            7540..7755
                     /locus_tag="VE030_11095"
     CDS             7540..7755
                     /locus_tag="VE030_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23996.1"
                     /translation="MSPRPADPPLDREWTQEELRSYLEPHGFTFREGEVIPQGCKYIS
                     FQPVTEKEIRWVEDLARRFDWAKRFGW"
     gene            complement(7862..8572)
                     /locus_tag="VE030_11100"
     CDS             complement(7862..8572)
                     /locus_tag="VE030_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23997.1"
                     /translation="MNFSVRENRNCQPRTGRAIREVNISQLVSGERLEPHRLVMVHVG
                     ATVIAGARRGGLNLLHAGPLPDVTDYRSIRPGPQRRVALDARQHEAGRAVDGAVAALR
                     GVRERDDPFDPRILAADQPHGHAQRAALGIARQVFAEGQPVQFQKAVPSGALHQPQQF
                     APVERRAELHGPFQARAQEHAQVQLGVHHHLVRVVQFADQRVAGLAADRGHADVFGGR
                     HFPDHFSEKMHATRMLRH"
     gene            8604..8679
                     /locus_tag="VE030_11105"
     tRNA            8604..8679
                     /locus_tag="VE030_11105"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8637..8639,aa:Gly,seq:tcc)
     gene            8834..9181
                     /locus_tag="VE030_11110"
     CDS             8834..9181
                     /locus_tag="VE030_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23998.1"
                     /translation="MEATMQVGARASVSVEWKDTGGNIVKVDGPTKWESSASDMVQVT
                     PSTGNPQIANLFAPGPIGTAQIHATADADLGAGVKPVTATIDVTVISGEAVGGDITFT
                     PTPSAAAQQPKRR"
     gene            9633..9896
                     /locus_tag="VE030_11115"
     CDS             9633..9896
                     /locus_tag="VE030_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF23999.1"
                     /translation="MTRRFVAPGDVVRLEIPSSEVCMSMHVAGEVMNVRFQSPHSYPN
                     AQLLMDDGREFSAPITPGEAGFYYEDGKYYCYPVDAQNNGGRL"
     gene            10093..10329
                     /locus_tag="VE030_11120"
     CDS             10093..10329
                     /locus_tag="VE030_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24000.1"
                     /translation="MSTPIIIVIGDVGTGGWWYAIEEQQIFGDGFPTETAAIAAAATA
                     AGAAPYEIWYRCEAEGRTVMVPGARSANHPEEGL"
     gene            10326..10658
                     /locus_tag="VE030_11125"
     CDS             10326..10658
                     /locus_tag="VE030_11125"
                     /inference="COORDINATES: protein motif:HMM:NF024013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3768 domain-containing protein"
                     /protein_id="HZF24001.1"
                     /translation="MSPDPKRIAALNDLARTAMGVCSRVLQTRGICALPPADQSRIRE
                     KVELFNDFHPGDDPYGEHDFGSFEHNGVGIFWKIDYYDTKLEFGSEDPSDPAQTTRVL
                     TIMLREEY"
     gene            10658..10969
                     /locus_tag="VE030_11130"
     CDS             10658..10969
                     /locus_tag="VE030_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24002.1"
                     /translation="MQRGTQVFVTGAKRFTGTVLAAGKDSLVIEVAPGVSATLRRDIA
                     STKPLQWRCAGLPVEVSAKPANNISGDCKPASIEGPKRVVNLKEFRKRLFALEKRTRK
                     L"
     gene            11099..11311
                     /locus_tag="VE030_11135"
     CDS             11099..11311
                     /locus_tag="VE030_11135"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZF24003.1"
                     /translation="MKRKKAGKAGTAADPFDKTKRDAAEFLGVTVSTIDRYVRKRKVP
                     HIKLNNHLVRFRISDLVKFAEKQRVA"
     gene            11682..11942
                     /locus_tag="VE030_11140"
     CDS             11682..11942
                     /locus_tag="VE030_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24004.1"
                     /translation="MTKPIDIFPTPVLSEDEGQLTLLFPGNVLGVSLMTDSALEERVT
                     QALIRAWNSSLLAFQREAWNIFTSGTRVPGAGWLRRSEDVSE"
     gene            12442..13044
                     /locus_tag="VE030_11145"
     CDS             12442..13044
                     /locus_tag="VE030_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24005.1"
                     /translation="MTETHLLTDAELQGIALQALNMAKRDLLQHKFNFLVGAYHHGEG
                     LHRMRKIETLLIETLGEDWLNGGRTKDAGFGVLRSAVERMPPDAVVFVSIVNQFMAAE
                     KLRTLGEEEARELLNSTHDRHHRAVAEGLLTVHDALYAIAQDPRRICMYVQMIDRHLG
                     FLGPPDIQFMDQDNFSGRMKMYGPKSGYTDAVRPARKAAQ"
     gene            13063..13380
                     /locus_tag="VE030_11150"
     CDS             13063..13380
                     /locus_tag="VE030_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24006.1"
                     /translation="MLWIATMYCENGHPIIGAVCVLERAGLPDERELDEFVQSARAFL
                     AEAAAQQRGTALCEDCGAASTALRLEQIDDPDFEKTVHEFAADLKKPSAKRREEQLWK
                     LRN"
     gene            13397..13621
                     /locus_tag="VE030_11155"
     CDS             13397..13621
                     /locus_tag="VE030_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24007.1"
                     /translation="MSVMTRVEHLAWAKQRALQYLNAGDLMNALASMGSDLSKHEELS
                     KIGDMLFPLGMLYVQNHDVGGMRRWIEGFN"
     gene            13624..14313
                     /locus_tag="VE030_11160"
     CDS             13624..14313
                     /locus_tag="VE030_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24008.1"
                     /translation="MTYEERFRGLADAARMLRVMMEAREAAAKTARFRPDGKYKPRIS
                     RNREIADRALLREPIPGGGGAEWWIAYGGMRENILELRKSPILPLVERTLDPEALNGY
                     VAFPKRHAPKLPTNSYGGILQYIPVHGGVTYAVKDSYMAVWGFDTLHCDSGEVPRADR
                     AWILGQCRLLHTGLLIAAKLWPEWRKASQARRTEMADWMLQLVDDGRVGLTGRLNLGS
                     MLDLMRGRIGE"
     gene            14310..14702
                     /locus_tag="VE030_11165"
     CDS             14310..14702
                     /locus_tag="VE030_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24009.1"
                     /translation="MTKATKKAEFKGRLEMLDPLWVQILEAKPNPLFGEDAHEWVRFL
                     GRSMKIRLAIRGYVGKRVMFEDRVEMEEAALDRLLPRLAEKHALALAEHALHMIEIEF
                     LDEANPLERFFRFGTDPAGMVLPIEVKL"
     gene            15112..15339
                     /locus_tag="VE030_11170"
     CDS             15112..15339
                     /locus_tag="VE030_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24010.1"
                     /translation="MSLSSEQYIRFGFYVGAAGLWLGDSSYEKRWRGKVEELTEELYW
                     EIVSEIQQRLVAAGKCRADEDIFETSLSEAT"
     gene            15341..15670
                     /locus_tag="VE030_11175"
     CDS             15341..15670
                     /locus_tag="VE030_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24011.1"
                     /translation="MSPLLDPAHPLAPKFWRNETGGELQPAVMAYLTGEPMTLRQIAL
                     MRAYLQQWIDSPVWNPPGPPDVPKGTCTDARLELEFIRAALAEVRTRAQINEWLHSAL
                     SIGIDPL"
     gene            15667..15999
                     /locus_tag="VE030_11180"
     CDS             15667..15999
                     /locus_tag="VE030_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24012.1"
                     /translation="MSDVYLSQSRTPLPHLRCPKCGGPCDGWSGLSMEEFAQPEPGSR
                     TLCGTCGAFLVFIESAASYRALALRMATAAEIAAICAHKDPKVRQLWTLAQEFVAKRN
                     QRQRTGQG"
     gene            16040..16600
                     /locus_tag="VE030_11185"
     CDS             16040..16600
                     /locus_tag="VE030_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24013.1"
                     /translation="MQSQKKPAKGKAEHYQIGTVTCAPLEQDLVRDFLLAAEKLTADD
                     PSQREEVEEIRARASKRGKSGYYTSKGKKQRKEDRKALWQILWNCSGPYCTFDEHPDD
                     PGNWGFWLISHWREELLEGHGIIVYEVRQIPKNFEGDFLLVTKSGNLIRKVAMCYRGA
                     APAHEMQTFWEMEGSLIPRSYLPQLQ"
     gene            complement(17117..17638)
                     /locus_tag="VE030_11190"
     CDS             complement(17117..17638)
                     /locus_tag="VE030_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24014.1"
                     /translation="MAKKNLNVTATVGKALATALKSAAKPAAAVKPAAAVKPAAAAKP
                     AAAVKPAAAVKPAAAAKPAPAAPAAPKADKPTEFAIHLNKTGRVCFGKDAAERLGTPK
                     PKFATLTIEGRMVRIVPQAKEVEGALDVRDASGRPYISATKQFKTLGFDGSRPYDIEA
                     KPYGSAGFEFKFA"
     gene            18250..18570
                     /locus_tag="VE030_11195"
     CDS             18250..18570
                     /locus_tag="VE030_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24015.1"
                     /translation="MICPECGIEVKRHVCRACQAKRTRAGMLRHQRRFLQTWLAGQIE
                     LRVKRVAGVLHLELFDDRWHSYCDTEMFSVHQRDFVRELPADLCPACITIFHEQVAAA
                     KEVR"
     gene            18567..18707
                     /locus_tag="VE030_11200"
     CDS             18567..18707
                     /locus_tag="VE030_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24016.1"
                     /translation="MIKLKLLLMILAVLCFALGASEVKPPRGNFVAAGLCLWALATVV
                     AG"
     gene            18920..19426
                     /locus_tag="VE030_11205"
     CDS             18920..19426
                     /locus_tag="VE030_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24017.1"
                     /translation="MPLVTELELARHLKRDRKAVRLAAQSGRLTTRADGLFDLDVAVR
                     EWAETTHHEKGHNNRSLAEIPSPDIPLRPETKSTDYAKARAGTQIYEALLKKLRYEER
                     AKNLTPTADVETARFTEFRILREACFNIPARIAALIAGETQVERCQQLLESELNSVFN
                     AFADGRLS"
     gene            19423..21357
                     /locus_tag="VE030_11210"
     CDS             19423..21357
                     /locus_tag="VE030_11210"
                     /inference="COORDINATES: protein motif:HMM:NF042573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HZF24018.1"
                     /translation="MTPALTLHASLPGLADAYEVSRQACRAGARPDPKLRISEWADQY
                     RVLTTRSSPEPGMWRTERTPFLRDIMDSLMPEAPWERVVFMKGSQVGATESGNNWIGY
                     VIHLAPGPMLVVQPTEMMAKRNSKQRIGPLIEDSPVLRTLVRTARSRDSGNTILAKEF
                     LGGILVLAGANSAKQLRSMAARYLFLDEVDAYPPNVDREGEPCDLAIARTSNFRRKKI
                     FITSTPTIAGRSRICTFFEQSDQNFYFVPCPRCGVLITLLPEQLGWSPTLKHQASYRC
                     QECEGEIFDHEKTGMLELGEWRATAAGDGVTRGYHLSSYYSPVGWLSWTQIMRNHDKS
                     TASPEKLQAFYNTVLGLPWADQGEVPDVDRLYERRESYVIGEVPEGGLVLTAGADVQL
                     NRIEVEIVAWGRDRISWSVDYRVLEGNSNQPQVWEQLARLMDEEFPTFYGGALRIQKL
                     AVDSGFNTMRVYEWVRTMLAQRVMAVKGESHVPALVGSPNLIERGPAGRLIRGGVRLW
                     PVNSSIGKEELYRCLKLSAPDLAAGEEWPAGYCHFPSYSKEFFEQLCGEQLITRTLAG
                     RTTSRWEKRRDRNEALDVRIYARAAAATLRMETWQPKRWNDIETALRGGRAIGGSVAK
                     GVQSPIPQFRPLRANEGFLE"
     gene            21354..21668
                     /gene="gpW"
                     /locus_tag="VE030_11215"
     CDS             21354..21668
                     /gene="gpW"
                     /locus_tag="VE030_11215"
                     /inference="COORDINATES: protein motif:HMM:NF014846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HZF24019.1"
                     /translation="MTPNGRLPFQPKPMAANVRFKLPKVTDPATAATMLEQAQQAMFN
                     LVTGQLPSGVETPQLGRVTFNATNTADLQRLIDYLSGVVASGDGSGNTNRRIPISFLA
                     WP"
     gene            21665..23344
                     /locus_tag="VE030_11220"
     CDS             21665..23344
                     /locus_tag="VE030_11220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HZF24020.1"
                     /translation="MTATPESNPTSSTTVPAIRKPGLLKRLFGGDRSAAQFQAQSPDG
                     GSNYGAGTGYGALGGRDTPYTGASWIRKQMSNWFPTRASADAELLGDMGTLVARSRDL
                     DRNTGIAAGAIQTTVDNVVGANLRLSCWPDYRALGRDPQWAESWGRSVESLWKSVADT
                     TAFDVANKLTFTGLTTLVFRSVLQNGEALALALWMARADAKFKTCVQLVDTDRLSNPG
                     NMTPTLTLRGGVETNDYGRPLAYHVRKISNWPAMFFPAIGGIGGEWERIPAATEWGRK
                     RVLHVYTQDRVDQTRGKPILAPVIEQFRMLDSYMRAELQSAIVNALVAGVIETPLDPG
                     TLAEMVGGDANGYLAAKNEYRIQLEAGTFIPLYPGDKMTPFMPARPAPQFAQFSEFVM
                     RQIGVSMGLPYEQLMKDYSKTNYSSARAALLESWRYFTTRRSWLCTYWAQPLYELWFE
                     EAVNEGLIDAPDFYNQRTLYTRAKWIGPGRGWIDPVKEAEAAQVRMSSGISTLEAECA
                     EQGLDYNDVIDQRQIEKQRLQEAGLWVEPAPPTKPLGFPAQPEETPVREQV"
     gene            23348..24721
                     /locus_tag="VE030_11225"
     CDS             23348..24721
                     /locus_tag="VE030_11225"
                     /inference="COORDINATES: protein motif:HMM:NF013506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HZF24021.1"
                     /translation="MATDELLTGEAPLAFQLLSLNDRPWAMSLTALRSLVAACGALDF
                     EAVAARAGQPLDNGSGVMNYHGTAVVNIRGPLFRYRSIWTWLLGGTSVEQTALALHAA
                     VDDPTVQRIVLAINSPGGQIDGINELANMIRTANQTKPITAYVDGQGASAAYWLASAA
                     GTIVADETARLGSIGVRATVFDERAAEEKQGVKRYEIISSQSPLKRTDPGTDQGRAQL
                     QQMVDAMAAVFIAKVAEFRGTSEEKVVSDFGQGAELSARDAVSVGMAEALGSLEGLLG
                     AGNGDPVRVVRDQPALRVGLGTTVPAAVLHIGAAATEPEAAEETDDEECDCPPGEDCE
                     CDEEEQEPLDSSASEGDGDLITPTEERQRIAAILTCEEARGREELARTLALETNHTLE
                     AAQKLLKACPLAVATKPVNALEVRMGQIQNPNVGVSGDAVADDSPAAEVQRILAFVPK
                     SRRHAVQ"
     gene            24731..25555
                     /locus_tag="VE030_11230"
     CDS             24731..25555
                     /locus_tag="VE030_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24022.1"
                     /translation="MATPSVNPINVASFRSDIFNYDSLLAGECLSKSAKIGASQGILK
                     RGTVLGGVAAGVPPTGVTPFVYGNAPAGAILAADIDTGTGAAVTGLVYTQGKFLDTAM
                     IFSANGAATDVAQLWNVGIYVLTVEQRSGQLVPLMNLPTSGAGPLPQAMSAKDAAEAT
                     KEEVEAIRAAQGQSPVAIPGPASAKDPAWYTAAFGERELTKEEQAREKTVEQVADLDE
                     KQQKAREELTAKQNKEASELAKKQRDERAQLAKQEAAAMQHGRQANPPVPTTAPKK"
     gene            25639..26661
                     /locus_tag="VE030_11235"
     CDS             25639..26661
                     /locus_tag="VE030_11235"
                     /inference="COORDINATES: protein motif:HMM:NF015802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HZF24023.1"
                     /translation="MADVFSTDVLTAVLQSLLGNPQFLLDRFFGITQAEASEQIHFDV
                     MQGKRRVSPFVSPLVEGQVVASQGFVTNTFTPAYIKDKRVFDMNRPLKRGPGEQIGGT
                     MVPADRLRALIAFDMQDQLNMLRRRLEVMCGEVLASGKSTISGDKYPTQVVDFQRSAT
                     HQIVAAPLWSTASPPILNNLQDWAQICLEDTGVFPNDVIMTVDVWKVFRADPAIVNVL
                     NIFRRNTDTPSVMPMAQVTEGGVLMGNIEGFNIWVYSGWYVDPADGVEKPIIPAGTVI
                     MCSPSLEGVQAYGAIRDEEIGLQPVPYYVKSWIQYDPSVRYVMLQSAPIMVPFRPNAS
                     LAAKVL"
     gene            26766..27101
                     /locus_tag="VE030_11240"
     CDS             26766..27101
                     /locus_tag="VE030_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24024.1"
                     /translation="MSSFINPFLSDYLPNLFWPAELATFGRQVQYLPQGDAGQAVAVT
                     ILWKEGASDEEVSPGRYSHIDLQNADLPVAPALGDIVQKDGKEYDVVRVNALAVGYST
                     IVLQEGGTL"
     gene            27101..27619
                     /locus_tag="VE030_11245"
     CDS             27101..27619
                     /locus_tag="VE030_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24025.1"
                     /translation="MAQGILAGPIAATQASMNIQVKRTGRVRAPKLDDPQLAAIGLKM
                     VAEQKARWARGMDAAGQTAKKLTVRYAIIKQAVLHKRPVRDMHLTGATIKNFQLRKAT
                     LGTIRAENTTRLERSKARRCQGYDQMIGFALTDTKVVFDAAQNEFGQYVNKAWIPLDG
                     TNRRPGTLNMLP"
     gene            27627..28088
                     /locus_tag="VE030_11250"
     CDS             27627..28088
                     /locus_tag="VE030_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24026.1"
                     /translation="MIKLADLVNAMAQTLTNIPELVAQLAPTDPVKAYIDFNPTSNSV
                     DNAIYQMQPGQLLVMWVETALVTEAMSKWSHRVEICVRAMRDHSDLDLVDQILNGVPV
                     PGDGQIWRMCPILAGVLPTDVLGIARRTDTEGVDYAVILTETAETGDWPLP"
     gene            28111..29202
                     /locus_tag="VE030_11255"
     CDS             28111..29202
                     /locus_tag="VE030_11255"
                     /inference="COORDINATES: protein motif:HMM:NF039819.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HZF24027.1"
                     /translation="MATLTAPAPLAGGPISCPANIQETRIAFGYKPQTDVPTINALAD
                     MWALTKVNPALSVVNPVNETNALDIGKGNEFPSQTFPSHQDTSVALEKYVSSEFLAWL
                     FCFTTGQATKTVAGTGFAYAAVPNDPAVTCINLPCFTWDEQIRPSPNSVVDRALIGCV
                     VGDWALTMSSGPGRANCRVACTLPGSGRVQSPGLSPLPALTPEHFLNASGAFITINGI
                     DYVLQQTFMSLEFRYNNNVRLDTGLYPGSGTQNGFGVRGRMEYGVREMSLNFVARAQK
                     GSIEYNNLINQVEGPVSFGVKGALIDTTFYHNFTISMPRTIMQAVVNGDDANIVTVQC
                     GVTALQPTDGITPIITMSATTTKDGILGL"
     gene            complement(29205..29918)
                     /locus_tag="VE030_11260"
     CDS             complement(29205..29918)
                     /locus_tag="VE030_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24028.1"
                     /translation="MAHRKAREITHRAFHFCRLRFASAYPLHPAALSVFRYNGTGMHR
                     FIFIVLIAAGAGYWFRLERPTAYNSGRAAMVPVPAAPLPAAKEPDQEAAQPEPEQWKH
                     GGARDDLDGTEQRFATLRSTNLVEFPFPYNGPQHGTLRVSEEKPGLFILSLHLERGQF
                     IGDWKSYGIVCPLRVRFDEGPVQNFTMLRFDDQTNAAWTGQSELIAQLQTAKRVRIQA
                     HYFEAANVTLDFHWDEFPF"
     gene            30046..30645
                     /locus_tag="VE030_11265"
     CDS             30046..30645
                     /locus_tag="VE030_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24029.1"
                     /translation="MFDTAATITIGLRVAGAGKTDITVRWPSDEEWAMHRKRSKLMMR
                     QMGRGASEMETDSSAADQKMYEAIKLNGAPPLTAGEATTIIQAIGKCDVLRVDLGADE
                     AEAELQILTGRVTHTVRIPTLDEVRKLHRTTRYLNLPYNCQEVRTSMEAAAALWDACG
                     GKADGYAGAVPILHKDSVIRQVIIEIERETAASYGEENF"
     gene            30750..31004
                     /locus_tag="VE030_11270"
     CDS             30750..31004
                     /locus_tag="VE030_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24030.1"
                     /translation="MRDPFADANSPPCDECPLALLDEYLATPAGRLIGLTIDLDFAMQ
                     AGVTVSLQDITYPEFLLLRFLSEERNRFHAEAAERAARGQ"
     gene            30994..35154
                     /locus_tag="VE030_11275"
     CDS             30994..35154
                     /locus_tag="VE030_11275"
                     /inference="COORDINATES: protein motif:HMM:NF040129.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HZF24031.1"
                     /translation="MASNTIYLQVDFNSETAQQNVNALNQSIATTGPTAEKSSKQATA
                     GFQSIGVSIQSVNREFSQLTSALAGLGIARVIAGMVQMSAELSRTQLAMQSFTGSAAE
                     ANKVFEQVRAIAATSPFRFKDLEETARQLLGFGMAAKNVPDTLRVITDQVGRMGGSIE
                     NVNNVVRLFGRIMEKDFVGAMDLMRALPQQGVQVMTALREAMSKQLGRAIDVEGVRDA
                     IKEGILDPLQTIRVVLDSMRKAGGFGASLADAALAFKNMADAAQNAVSQLFGPQGFGP
                     ALRKLGTEIGNLLAPLGGLLDYLMKLPDPTKLVILQVLAGAAAVAALGTALAIVTSLA
                     SPLLSLTAAIFKFTVGLATMNPQLAITVVLLGTLAFAAYKLIPQFQGMVDTLVGGVAG
                     KIKTALTGLAEQGKKFMAGIFKGGGQIEPGDILDTAKLFEKLRLEEQKHADAAQRSLL
                     EALASPVEAVRLKYATLFSELEEKIKLLPQAQQQALRAILVGAEVTETAGAKFKEQKK
                     QEDEFTRYQVERAKGAADAQVAYIEAQDAQDLRSKVRNIDRITQIRMDAAKQVADIEN
                     NNLEDQFHRFEKMANDYRKQFAAAGVDVDKMIEAHRKETDDKEAAVTRKQFDEGQKYR
                     LEGWKKANDLIIEDQKRVYDAFKDQFSQLFDVFTDKTKSLGQALGDFFKKLVLGEAKE
                     LFASQLAGVATAAAGYGQPQESLGRGGQGILGTLLRRGMPPRPPLPPPDVQQINATQS
                     DISFPVASAFQNSTHSYEIATNAYSDATIRFAEAVNNFAGATVQHEGSADRMADTTTT
                     MKEAFQTASGATGVPQTLLRAVAQAESSMRPGAVSSAGAMGLMQLMPGTAREHGVTDV
                     FNVGQNVMGGAQHLQEMLTRYHGNVPLALAAYNMGAHALDQRLASGRALPAETKAYVA
                     RITQLMAAAPEAAGGGGADLLPGGEDARYLAATGGGGAPAVTMAQLAALPQRAQPLFL
                     PGVPSFQAEGAGAVTGPSVAELANLPMGLTALQRLSPSLLPLLTGALKAGGATTGAGG
                     VNLAGLNIQNLSKLGQFFGIYGKGVTSAGSILTSPAAGTLALMAGMGLASKGLQQHYA
                     PATVAGGALAGAGMFLTNPSMIARASEMPGGVGLGIGAAAATGAGLGLFASGFQRGGG
                     TGLAMDIGGGALAGAGIGTMILPGLGTAIGAAIGAGVGAITGIVRLFVHTEQERIRTG
                     IKQVYGIDISNRQILTQIQQIVDQKYGGSVTIGIRSQDVQDIVRLYALSTGQAANLPR
                     PMYAATIAQSQAGGLATQPVYQGGVLVQNPYTGPTTYQYQTAVTAAQGLMAGTSQGVP
                     GAGGQVSSIFMQLNPQQANDLFTGRVVQAVQQNPAAVASANASATRAGDSRVSQASNM
                     LEPLTSLS"
     gene            35386..35781
                     /locus_tag="VE030_11280"
     CDS             35386..35781
                     /locus_tag="VE030_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24032.1"
                     /translation="MPQNLNSANPQGVMPKQLCRSFQEELRLEALLNQYADGSSDRNA
                     LAQNVRHFFRLVPGVNGADWAALWQFYIQHQGPAFYFYNLRETVPPFSYDPSGQNTIG
                     RYTVVFDGQWSDTYNVARTDVALQLREVA"
     gene            35817..39863
                     /locus_tag="VE030_11285"
     CDS             35817..39863
                     /locus_tag="VE030_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24033.1"
                     /translation="MPIPSPPVISAFPLRPDFGGGIDYNPAVVVHTFDQPGLKTEQRF
                     LLTPMGARRFKFAKHHLSCREYDDLKAHFEQAQGSYAQFPYTVWEPGPGHGVPRPTQP
                     VYATETVTARYENPNLTFDYMVALLMNGPGLSFLEVPQTTPQYYSAQRLTRFPDATLT
                     AALQVQFQQIIPLITVVPRSNPTAAVYLSNQHFALDSQSYLPRLLDWSGISQSIGENS
                     DSASFTFGNADGVWQTYVNQVNLYAASVQVSLYHVGSRYILDLWKGALLNWGFDTSGR
                     FQVNAADGVFNLSLAYPSRKILRTCWKVYKGRWCPSTADPGTFPDCPKDYDSCVARGV
                     PHSFGGLVVPPQSVHIKDNSTGVFGFGRSSMTSVTVTQDTVYQRPLQEIFTDTPMNVT
                     VDVAAGRDESDFYAALGIVGEGPISAYNGNLILQTLDGQPPQDPLRFSGWRAFTGTDP
                     SGPYDFVGISQANPLTGKWANPDGSPYVPVGSTYAGGIALAEIRRTDQKGLQLAPVAD
                     RAMTVNVTGGIGGWVWNGPGQRTWLNSLHNAVWVAVNVYLRGIGLRVDPSNAAQVPPS
                     VMEQYVDVDQAGIAAAICDTMVPKIIGAGNELQFPFRGVLKEQKPLRDWLREILNCCL
                     GYFTFVNGKLWIGIRSNSSVLAGNAFTRASVLFKSLTASPLQPQFNWLVGNFGDEEFG
                     WQLNNVTIYDIDHASFLGTPDSPQYLTNTVSFAGVSNKSQCARVIITKLREEIGGLKS
                     GSGPHGTGSGIDEQMNARNFQFRTTVLALGTLVGDIVSIAHDTMPNSGYAEGRVAKWT
                     LNPDLSIDMQATSTTEDMYDLVVGPKPEDVSAPMVPPEVLQAATGLAWMPNEVAPSAG
                     DPVYPPWERTFDLWQEYEISRDAVWEPTIWVQGEMAINQFAILKQPRILEIEVQSGGN
                     LTGPLTVYAAVTQKNAGGEPAVPSNLTAIYIPTGMSNQKVTLTVVPAVDATLTDYDVW
                     AGLDRRRMAWQFGGSGQPPGTIDIPGPIHDMTRGLPEGAAVGVRIAAKHVWHAGVAGL
                     LVNGVTSPNKILCNDFLHSTDDWVGQMVFVCSNKDGQVPLWNFEITAFDPATGVLTVS
                     PDCATADPTQSVQQGDVLIVYARPTSADANSISNSLWDNSVNRQQFPGTAGMTPGEEK
                     GRIVRILRNTGAGQWRFVTDNTALSHSISPPWDVIPDSTSLYIVEAADWPSISPTNQL
                     IVSHSGISTALHLQVPNLTDEVALVGGFLVDTEGRQTDDSVAAFRMIFVFGQPPTVRV
                     VGPDRGPFDVAVTDQTIRADTSQNDVTVNLPPLAVLQGRSLLFVTDGPNTTMINTSPP
                     DKFPDGSVRIALGAGATVRITAGGIYTT"
     gene            39911..42997
                     /locus_tag="VE030_11290"
     CDS             39911..42997
                     /locus_tag="VE030_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24034.1"
                     /translation="MPKASPTTGSWIVETDGTGSSGGYIPPDAPPVSVGIPEVNPLYD
                     GRFEVRVPWTPDPTAGSGNFQGVSVYVEDPDMSETTTAPMDGTVPMDGTIQMAGKWIW
                     QHVTENTESPADLFVNGKVSDRYIRVYLQAYNFVVNSTLIRANKPNATPNVRVPIPAA
                     AGHYVSGQEYAWLVSNPIVTVIDNFDNPEDPTYSLVFSFIEPDDTIPLPPGLKPYAGV
                     QTVYEYPSQNNRRAQARFLTAHKPETWISDEYYATTITFLVWFCSVDSEGHVNTIVKG
                     VTPMVQVSVTYPPAGQTISPEVTGLAIGPTTYLPTPDGSILAQAQLTWTNPATLNRFS
                     GIHIYRVGITPPRSFGVGGNNATSFTLEALDWPPVTAESWTIAAISLDDKGRLADDPA
                     SISAATPTAIWHIGPPSISNGQYAPVVVATAATVVAEQQLNSDGVVMVRFVISGWVNS
                     TDNKFGGVSIARLLANDSLTTGKTTWWDAPKNQTSFTTDWEPAPAATSWDFWFVSRDQ
                     QGNRNQILPGITPKVTVNFTPQAGDVLASRLPKDWFDTSEFQWPDGFDFSVKLIHAEK
                     IFVDKVLRVGGGTGTLASSFAGNQNGQIGVFNFQNTLVGWIGQQDGTSTGDNPTPHSI
                     YGAWFAQVYIGGGNPTTAPIYINDQGVAIFGGFDTQIANGATRYPYISIRDASNVEKG
                     RIGARIGDGLVTPADPAYIAGAWFTEFAVGGQSLADWRILAKRSATTDPTEGSLVQLR
                     NINMFQINYVQNYHYPSGGSNHPNAAYTFQLGYDAYVTDGTTSAYSKFPGLTLVRDGT
                     SHGISLINRGLICYMPNGARAAALVTYNGDPNGGDGGQFWAEMTLYSQANSAINVELS
                     SGTSGSGASFFKLWDAGSNLNFSVDGAGTVFCRSGLTCASLTVNGALTVNSLTVNSAM
                     IAQSYKVGSTEVITAGGQFDGPGGVNTTGDIRTGGQVVGASFYLPGGSQPLITNAGGG
                     QWNGAGGVYTNGEIESTSVVKCRTGFYVNGVGGANGSFVAGAKTVTVSGGIITSIV"
     gene            43012..43404
                     /locus_tag="VE030_11295"
     CDS             43012..43404
                     /locus_tag="VE030_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24035.1"
                     /translation="MERSFRLLDYEAEQQTRMEQELQNTLANYGALTLQLEAVKARIP
                     QVEERQRTFVGQVAARANAGQFQSARIANGHLICTVPDEPMGLPNPVPAGLPVLDSAG
                     GPVAVPEHRAAEGRKPRVNGPDQISAKE"
     gene            43409..43678
                     /locus_tag="VE030_11300"
     CDS             43409..43678
                     /locus_tag="VE030_11300"
                     /inference="COORDINATES: protein motif:HMM:NF021255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbcA/ParS/Xre antitoxin family protein"
                     /protein_id="HZF24036.1"
                     /translation="MASDCSDVIRRTIEVIGNRREAMQWLGTPVRALGYATPIARLAS
                     EAGTEEVLNLLTALEHGVLEPVGITGTVGVITLVGGIVVEYRLKE"
     gene            43685..45922
                     /locus_tag="VE030_11305"
     CDS             43685..45922
                     /locus_tag="VE030_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24037.1"
                     /translation="MPWLSLNTYVPTDKVRNDMFNSLANDDRNWGGNVNGGGNRLSNV
                     ILDGTGGFQFYASPLQVTPGADGQGVVQMDQTVGANQIARWTAGKTATAESGANAGSD
                     YAIVRYDDAGALLGTPFQIARSTGLITMGAQKWTGAIDGGGQTLSNITIAGVASDPTT
                     TKGDLIARGAAVLGRLAVGTNGQALVADSAQTLGMKWATTDPTTTLGDLIVRGAAAVG
                     RLGVGTNGQVLQADSTQTFGVKWAAAATTASYSKDGTLVGTRPGFNVITGANVVLTMA
                     DDSANNRVNLTIAAAGGGSGGMVDPTTTLGDLIVRGSAAPVRFAVGSNGQVLTADSSA
                     ALGVKWAVGGGGQTPWTSDINAANFGLWNLRYLAIGAASLQPSPTVGFGVAGSSGLTS
                     VVITETLSTGQAGFTLNANVAQATFMLSGSAASVPNHLALSNNTGGISFWTGTERLRI
                     TATGNVGINITTPGSLLHLAGNGAVDTPQGVITMSRFWPDASNVRASALFHYYNSAAA
                     TDLLAFGVSGDGGGSTTAPGQISNAKMVITAGGYAGIGTTTPTLEAAMARLVVTGIAA
                     QVASNLATSNTKALASFRANSSSGYTLAIGAESGNSFPYLQAVNWNGGSSAASLLLNP
                     FGGNIGMLKINPAYALDVAGDVNCTGAFRVNGVAIGGAQTPWTGDVDAAGHALNNVSA
                     VYSNIASVGGTGKYLIFGCPLATGDPGTMAADTIAFWRDSSNQLHIVCSYSDLTRHRA
                     TIGLA"
     gene            45945..46238
                     /locus_tag="VE030_11310"
     CDS             45945..46238
                     /locus_tag="VE030_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24038.1"
                     /translation="MALTYDQSNTLMQDAAFRGRVKVACLKYADSILNEAPTVPAHNT
                     RYRWAQQCDQQPDQTALTITPPTVMDPAVQQDGSNITDTALQGSVETVINKLL"
     gene            complement(46235..46489)
                     /locus_tag="VE030_11315"
     CDS             complement(46235..46489)
                     /locus_tag="VE030_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZF24039.1"
                     /translation="MPSVRLAFGRAIRALRATAQVAQERLAFDSDVDRGYMSGLERGL
                     HSPTLDTVLKLLWKLDISFVEFAIVFERELKRLKRSRPKP"
     gene            47157..47375
                     /locus_tag="VE030_11320"
     CDS             47157..47375
                     /locus_tag="VE030_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24040.1"
                     /translation="MQLPVGLIDAMRKLRHIRHQQEGVDPKLCRIYKEAVEQYVNAKP
                     QQQLLQEWYGDGPGKPARASGDRAAARV"
     gene            47485..48039
                     /locus_tag="VE030_11325"
     CDS             47485..48039
                     /locus_tag="VE030_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24041.1"
                     /translation="MQTATIEIDRSQAMTLYRQYKEHRDCFTKMDAEIERAYRQIARG
                     HKVIQALESIRHAGLDALSRPRLGIVRADAEWCYLTRGNGQVLFSMDRWVRARAPRRS
                     VIVRWPGMIWNIDNGGAKARVPLIPVHLRPRVSLDRYHLLFEADWVGVPPDPMLLRRC
                     GNDLWVVVAAWDLTPVERAVMGAE"
     gene            48044..49027
                     /locus_tag="VE030_11330"
     CDS             48044..49027
                     /locus_tag="VE030_11330"
                     /inference="COORDINATES: protein motif:HMM:NF021128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HZF24042.1"
                     /translation="MIDHQLRNACITSTDVGPIFGVDAYRGAFDVWAVKHGHAPRWNP
                     TPRMLLGKDLEQGILQAYSRITGRPVEWFDKTMRHPERPWMAASPDALVTSDPLERGV
                     DAKLVFWDQRRKWGANSNDIPEGIQMQMWWFMAVLECDVWDVAAWVGEDEPRIYEIER
                     DREAERVVIAKCEEWHRRYVVGDEVPPISGSETSSAWLQQAFPHDHRPDMRDATDAEI
                     AYLEEYALARIDQQEATERRGEMENQIKLAIGDREGLQWPGGKFTWRRTKDQEKINWK
                     ALGTYLLNTRIREEQEREAMRVEYTNTKAGVRRIHFVCDTHDISDAVGVTG"
     gene            49024..49272
                     /locus_tag="VE030_11335"
     CDS             49024..49272
                     /locus_tag="VE030_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24043.1"
                     /translation="MKIEQLSPEAQDGLRKWAAFQHDDLLFDAISSVPQERAWIWFQL
                     CAAEDRPEWFPKGKWATIQHIATILDEEADATRIRPSM"
     gene            49244..49429
                     /locus_tag="VE030_11340"
     CDS             49244..49429
                     /locus_tag="VE030_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24044.1"
                     /translation="MRREYDPQCEALARYFLGQPARDEALVVELAGVIQEAIEDWEQN
                     RQKEKTSDATDTKQHNR"
     gene            49437..50321
                     /locus_tag="VE030_11345"
     CDS             49437..50321
                     /locus_tag="VE030_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24045.1"
                     /translation="MTPPTAPPPAPGAIPTEARSRVTVEQLATLPDSERRTNWINSLV
                     DAEVARQNYAQDRAMAREFALCGQFDDLKGVSTEQAVATAMVKINLGRSWGFNCADSM
                     RNIYFVNGKPALEQDIVASKLQQAGIAWDPEFAYEEVPATKTGRAWRKCVGCTLWLKE
                     WNKIEQRYQPIKDRKGEQVAVSFTLADAENAKYWEKGQEKPLSEKFNYRSYPGDMFYW
                     RCISRVRKYYAPHVLRGGMLREEALEMMPADLPPDQLPRELQPPEVLPAERKTLAERV
                     KANEVGPDVGTQNAMFPE"
     gene            50495..50698
                     /locus_tag="VE030_11350"
     CDS             50495..50698
                     /locus_tag="VE030_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24046.1"
                     /translation="MSRKRQWGEDSTTKIDLSGGASITVSFLGNLFTLEPAERQLMSD
                     LSAVLQSYQDSKASGQAKAAALK"
     gene            50695..51282
                     /locus_tag="VE030_11355"
     CDS             50695..51282
                     /locus_tag="VE030_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24047.1"
                     /translation="MTTASTTRKCACGRTISGNKLTCAACSKPVATGDGVLEILNVGA
                     GDVKITFNTGDVSDTIRARRIVTDMLRRGFALLVEIERDGERKYERVQAFDEKTGEYL
                     IADFDPLDARQADLEDAATARRLETAQRHGEAPTGLQPPGSSSDLGITVKDIPLCACG
                     RPLHHRGSCKGQKRGYRRVPMEGAKATAVGRSSGG"
     gene            51326..52003
                     /locus_tag="VE030_11360"
     CDS             51326..52003
                     /locus_tag="VE030_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24048.1"
                     /translation="MAEKQHEWAGFPMPVEGMSMQIHPSYPFAENLSKTFLPKPSLRM
                     CRDSDVCEETRLRNRWNSYRDGKEILIVQERGKFFFLYSPVQNSGPMLLQTVSAARAW
                     DFEAELRAMETLKRHVTEWAFQCYVMTGSFLETSKRSGVVYLFRRLRPTIALTGKPDK
                     NRRDVGMRILCTLCAHPIGYYRDSWAGALVPTDDVLSHLLLMRADEKFFWRNSNQHPA
                     WAPESGL"
     gene            52215..52883
                     /locus_tag="VE030_11365"
     CDS             52215..52883
                     /locus_tag="VE030_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24049.1"
                     /translation="MRTIEVELTSEMPLLMHADNIDWADKMEDWKNDPKNKSKSKPGD
                     DRTPAWRWLGCLNYDDPKTGVVTVPSEYIMRCLMGGAAEVPTGKGRKTFKELSQSGIL
                     CEKFHWPLLINGEKKPVRMEDINQLLKVPRFPDQVEGAKALGFSLFVKRARVGNNKHI
                     RVRPRFDDWSTRGELMIIDDQITNDVVASILDISGRLKGLGDWRPSAKTPGPFGTFKA
                     KIVS"
     gene            53039..53491
                     /locus_tag="VE030_11370"
     CDS             53039..53491
                     /locus_tag="VE030_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24050.1"
                     /translation="MNRLKDAFPVPMLAEGRVIEHADLEEIVKAKRGGQRYYGVINSW
                     INSMKNTNGIFMTWETSVGVKVLGPAEILAHAEIRTRQKIKQTGNAVKTFVWVDRTRL
                     DSTGQQRLDHQERVANAIRDALNTARKDLAIDLAPVKSLPKPKMIREA"
     gene            54215..54364
                     /locus_tag="VE030_11375"
     CDS             54215..54364
                     /locus_tag="VE030_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24051.1"
                     /translation="MQNPKVPTVRVVSGHDLDLSRRSALRLLRLLERLAAKRLAREAK
                     RDAKD"
     gene            54348..>55008
                     /locus_tag="VE030_11380"
     CDS             54348..>55008
                     /locus_tag="VE030_11380"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HZF24052.1"
                     /translation="MTRKTDLPWFVYIRVSSKDQGDKYGPSRQIEAIRNWLQVNVPHV
                     RVPGLDTCIAGPRDVRQCEYVGFDKQSGKTDERPDFQRGCELAKAGRIGGFIALRLDR
                     VARNAGDAYLLRSRMKRMGVRLEFATQTFDATPTGDLMYQVYASFAELEGKLILERTA
                     DGRMGRLCGDKLFHNPNAMPYGYLYVDEAVAKKNPGAVIGRAIINEQEAKIVRLIFRW
                     YV"
BASE COUNT        11250 a        16491 c        17725 g         9542 t
ORIGIN      
        1 atgcgcctca accgcaaggg gatcctgact cgcgcgggct tggcctggtg gcgggaatcg
       61 gtgacccagg tgctgcgcaa agccgaccgc tacgcgggcg tctatgtggt caggctgggc
      121 atcgaggccg ccaagcgcga tcatgaagaa cgcgtgaagc tgatgggcgg aaatgcgcag
      181 cctctcgatc tgtccggagt gacagaagcg cggctggatc ttcccgtgat catcgacgca
      241 gaaacggccc ggctggcacg ggctatccag acgcagaaca agacgaagag ggcggggcgg
      301 ccgaccaaat acttccccct cagcaaactg gtctattgct ccgtacctaa tggaaacggc
      361 ccctgcggcg gcgtttggta tttccgcaac aatggcgagg cgcggcatgg catcggctat
      421 tgctccaaac acaactacca aggcggcggc atccgcctga agtgtcacgc gagcgagatg
      481 gtcgcggcaa agctggaggc gatcgtgatc gatgccatga aggaacacct gcgccagccc
      541 gaggtagcgc acgccgccgc catacaggct tatcgggcag agcacggggt ggatgcccac
      601 cagcggcggg ccgactccga agccgccctg gaggctctcc gggagcagca ggagcactac
      661 ggcacgatca ttctcaatcc ggccatgaag aagctgcacg gcaaggccaa cacgcagttc
      721 gcggccctgg aaattcagat ccaggccctg gagagggaac tgcgccacgc cccggtcagc
      781 atgatgtatt ctgaggcttc catcgtggcg gcgttcggcg caatgctggg cgatctggaa
      841 gaggtccaga ccttcgagga aaagcgggag ttcctgcaat cgaccgtgga gcggatcgag
      901 atgaccggag aggaagtcgt catcaggggc gcgatctcaa tcccgcagga cgcggtcagc
      961 ggggggcaaa attggaatgg ctgtggtccc agcgattcca attttacccc ttcgattccg
     1021 tttgttatca agaggcgggt cgcatgaccc gcgagcccac actcaccact ttctgcgcgg
     1081 cctgcgcagc ctgcatagcg ggacgccgcc acgcaccaga agaggtccag gcgcaccatc
     1141 cgttctccgg ccacggcttt tcgccagagg ttggctggac ggatccacgc cttgagccga
     1201 agcccaccac gttgcactcc tggccaatgg aatacgaccc cacgccgttc gcgccaggcg
     1261 aaaccgcggt tgtccccagc actaaggggc gggttgcgtg accggcccgg gcccaacccc
     1321 cgctggaaac ggcgggggtt tttcgtctcc gcagcgctgg gcgcggattc agtattcagg
     1381 cggggcaaac cgctggcacg cagtagacgg cttccgggcc atctgcggac atttggactt
     1441 caaccatcct cgggcggaat accaagagtc caagccggat cgcggcaaac tctgcaagag
     1501 ctgcgtggcg aagctagcgg aggcctcatg agagtggcct accgggacaa cccggcaatc
     1561 gccggcacgg tgaacctgat ccggtcctgg gagcgcggcg agatcgcgga gccgacgatg
     1621 agcacgtcga gcggcccgaa ggctaccata cgcaaccaca tcttcgcgat cttcgagcgc
     1681 tatccccacg tcggccgcgt gctcatggtg atcggcaaat attcccagag tgttccgttc
     1741 ctgctggtcg tcgacgaggc tggccaattc ttcgacatgg cgggcaaaga ggtagtggtc
     1801 gatggataaa cctaaacgct tgacggtcgc gtatctgggg tggcaccgtt ccgagcagaa
     1861 cctgactatt tgcgctaatg acgggcgcgc gatcgtgcgg ataacgcgcg acggcaaagt
     1921 aatcgtcaac cccgatttca cgattgacga agcagcccgt gccttctggg aagcggtcca
     1981 aaagctggca gcaaaggaga agtgaatggc agagaagcgg ttgtatgaag cgatcaaata
     2041 caccttcggg ccgacggaga tgcgggagct gggcgagagc ctggctcacg aaaatcaaaa
     2101 ctgtttcaac cttgaggatc agaaagcgaa cacggtagcg gcgctggcgg cggcaatcaa
     2161 aagcgccaag tcccgcgttg tggaactgac caccaaaatc aataacggct acgaaatgcg
     2221 cgaggtcgaa tgtctcgcca tcctagagac gccgcggccg ggtatgaaac ggatcatccg
     2281 cctcgatact aacgagatgg tccgcgaaga ggccatgacg gtgaatgaaa tgcagtcggg
     2341 cttcggcttc atcgccccgg gcccggaggc accaggcgaa tgaatccggc cctcggctac
     2401 ggcgtctgtc cagactgcgg cgccgtgtcc cacggcaacc tcacctgcag aacctgccgc
     2461 cagcacatgc ggcaaaccgc cggctgtccg gcggacttcc tggaccagct ggatgcggcc
     2521 aatatcgcca ccgttgcgcc ggtaggacca gcagcgatcc gcgcgttcct gaagcggttg
     2581 gagggcgagt gatggcgaag cggccgttcc ggctgacccc gccgccgatt tccgaggacg
     2641 acatagaggc tgggtgtaag actgtcctgg cgctgcacag ctatttagtg attcgcctgc
     2701 atgccggggt ttacaagacg ctggatgggg cgcggcatgt tcacggtgtt ccgaaaggca
     2761 cgcccgatta cgcctgcctg catgagttcc accgcaattt tctgttggag gtcaagcgtc
     2821 ctggcggaaa gctgaacccc gaccaggaga cccagatcgc ggcgatccgg cagcaatacc
     2881 gactgccggt ggtggttgcg gagggagtgg aagagttctg caattggctg gcgaggcacg
     2941 aacacccgcc ctaaaaaata aaggggcacg ttatccgcgt gcccccactg caatcacatt
     3001 ggtcccagct ggagaaggtc cccgacatgg aacacggttc caaggagcga actcgatgga
     3061 acgtaactct tttttgattg tagccaaagc ggtcctggcg gacaagagaa tttccgctac
     3121 agccaagtta cttctggcgg tcctcgcgga tcactggaac aaaaacacgg ggcagtgtaa
     3181 tccccatgaa gcgactctgg cggaggaaat cggcgtttgc cgccgcaccg taattcgcgc
     3241 cttggcggag ttaaagacgg ctgggctgat ccaggtgcaa cggacccagc ggggcaatcg
     3301 gtacgagttc ccgaagtgcc aaaatgtcac ttcccaagtg acaaatagtc actctgcaga
     3361 gtgccaaaat gtcacttcgg aacctccgta tcctttatat gaaccttact taggtgaacc
     3421 tagcggctta cgcgcgcaaa aacggcgcgc tcccgccgcg tctcctccta atccaaaacc
     3481 tctccacgcc ccgctgccgc gccgtaaatc ggtgcagagc gcaacgctgg aagcgttcta
     3541 ccgcgaagag cgaagaaagg caggaaggtg aatgtctgcc agcctgatag tgcagcacat
     3601 aaagacctat caagatgatg atcgcgtgcg cgacacggat ctagctgagt ggcttggcta
     3661 tgctgaccct cgcaagattc ggcagacgat caacaggcat cgaacgcaac tagaaaaagt
     3721 cggagttatt cacacggtgg gaataacctc cggggatctt gggggccgcc cagcctcaga
     3781 gaattggctc acagttaaac agtgcctgct tctctgtacc tatgcggaga cggccaacgc
     3841 cgatcaggtt cgcatcgctc tcgtggaagc cttcgaccag tacaggaaaa gtaagctggt
     3901 tgccgtcgag ccgtccttgg aaatgtcagt tctcgatact caggcccgtc aaatttgggt
     3961 gccgctccag cgcatggtgc gccaggagat cgagcctatg cagaatgacg ttcgtgcctt
     4021 gcgcggtgac gtccaagagg taaaacagat cgtccgcacg gaggcagacg atatcaagcg
     4081 cgagatccgg gaaagccgtc agaagtacag atggaagccg attgaccaag ccgacaaact
     4141 gctccatatc gattgcttat ggagacggca cgccggaatc tgcccgcgct gcaagcgcac
     4201 cgtcatcgta gatcaggacg gagtgccgac cgccgagttt gagatcgatc acaatttcgc
     4261 cccttggaaa aatgccctca cagaaacgtg gccattgtgt tcgcgctgca ataaggaggt
     4321 caacgccgcc gaagaacggc agaagtgcca ggacgatttc cactcctatc agaaatcatt
     4381 gcaagcccac cgcgaggatt tgcactttcc cctattgagg catttatgaa agcgttgact
     4441 cctgaaaccg ccgccgctgt cgtcggcaag ctcgcgttga tggctttctt tcctagcgac
     4501 cccgaggtcc gcgcggggct ggtcgccatt ttcatagaga tggttgaaac cgacgagcag
     4561 atggagtggc tgggtagccg ggcgatgcgc atgtttgcac gctggcctgg cgtggcggaa
     4621 atccgcgcgc tctactgttc gcggtacaaa ccgagggacg gcctcgaaag ttacagcgag
     4681 atttacccag agggcttccc aactgaacga aaagagcccg agccaggccg cgctgcgcgt
     4741 cgcggcgagc cggtcacagc ggacccggtc ctcgaccgta agattcggac agtagcagcc
     4801 agcaagaagc tgaagcccgt ccgaaagggg cgataaaagt ggagcaactt tggccagctg
     4861 acagcgtgga gcggtggccg ctggcgcggc tgatccccta cgcgcggaat gcgaggacac
     4921 atactccaga ccaggtcaag cagatcgcgg cctcgatgcg ggaatggggc tggacgattc
     4981 cagtgctggc cgacgaggcc ggcggaatta ttgcgggcca cgcgcgtgtg atggcggccg
     5041 agctgaacca gtatgccgaa gcgccggtga tgatcgcgcg cggctggtcg gacgccaaga
     5101 agcgggccta cgtgctggcc gataacaagc tggccatgaa cgcgggatgg aacgaggaat
     5161 tactgggagt ggagctgctc gagttgcggg acgcgggatt cgacatgccg gtgatcggct
     5221 tcgacgccag cgaattggac gggctgatgc gggaggtggc cgaggccggg tatccagtgc
     5281 tgccggaagg cgaccgggcg ccctaccagc agatcacctt cctgctgcac gacgagcagg
     5341 tgaaaatcgt gcaggccgcg gtgaccgccg ccaagcagcg cgggccgttc gttggcactc
     5401 ataacgacaa cgtaaacggc aacgccctgg cgcggatctg cgcagcgtac ctggaggagg
     5461 agaactaact tgcagtcgcc atcaagctac gacccggatc tgcgcagcgc gcggcggtcg
     5521 gcacttactt cgggcgtgct caacctcgcc gttgtgggct tccatgtcta ccggcacagc
     5581 tgggctgtcg tggcggcctc ggcagtttgg acactgtcgt cgtggatttg gctggtcact
     5641 attcgcgcgc aacagactac gcgcgattat agccgcgtgg tcgaggccgc aactcggcgg
     5701 gaaatcgaaa gaagggagtt tcgatgacgg atcgcttgat ctgggcggaa ctgttccccg
     5761 gccagccgtg gccgggtgaa gaggcagtgc tgcgggcgct ggccgcggaa gtgcggacac
     5821 taaggagcac gattgtcaac gagccgcagc tgcggcgcac tctcgacgag atccagcggg
     5881 cgcacgatct ggtgggccaa tactgctacg agacccagga cgaggcgacc ttccagttcg
     5941 tctcggctct ctgctgggtt ctgggccacg atcacaaccc cgcctttgcg gacacccttg
     6001 caacgattga gacgctgctg gaggggctcg gagtccaggt agtgcgcctg ccgcatctgg
     6061 tatatccgac gcggggagag agcgtaaaat agcggccatg caatcacaga atgagtaccc
     6121 tgcgtccgcc tcccggagct ttgccactgc ggcgatcgtt caagcattcg ctaagaccgc
     6181 gcggcctttc atgcgccggt ttatgagtgc caactcgtgt atcgcggcgg cgcggaccac
     6241 catcgagacc atgcggctct accgcctgcg cgcggtcgag aagccggtct gttacgtctt
     6301 tcaggtgccc gcgcggaatt atgcgcggct ggctggattc tccgcagagg agcgcgcgga
     6361 gatgaaagcg aaagcgggaa attgggtaga tgcaacgccg gacggaggca gctggaacgg
     6421 tcacctgatc gtggtggtgg aggaccgctg gattctcgat ccatcaatcg atcaggccga
     6481 cgctccccag ttcggcgtct cggttcctgc ggaggtgttc gtcgcggata cgcttggaca
     6541 aacttggcat ccgaatatga acttcgagat cgaactcggc ttgatcctcg acaacggcga
     6601 tgcggcatcg ctgctgtatc acagcatcag cgaccgcggg tatctcgaaa cggaagcctg
     6661 gaccgatgaa gggctcccgc tgctggcgca cgccatcgcc agcgagatga aaaacgcagc
     6721 ggcggtgagc atcgcctgtc ccaaatgcgg cagggtcagc cataacgcga acgacgtgcg
     6781 cgagcgctac tgtggcaact gccactcctt ccacgacgat gcgcgctgaa attctcgaca
     6841 gagccgtggc ctttgaagtc cagccggaag ctcgtccgga cggcagctgg gagccgccgg
     6901 ctgaagtgac cctgacgctg cggccctgct ggcgaaagcc gccggttcgc ttgcgggtag
     6961 cctacctgta cacctacgag caagtcgctt attacgcgga ggagctatga ttcgctgtgc
     7021 catttgccaa gacgatggtt ggatcgccta cccgacgttt gccgatgccg aaaagcaagt
     7081 catccgcatt cgcaaagtgg cctgtccgcg aggatgcccg ctggcgaacc cgaccgaaga
     7141 gggcgtgctg gctacgttcc cgtcagacca ggaaggcgag gcatgagcac caaagagttt
     7201 tgcaaacaat cccggctgac cccgcgcaag gtgcaatggt ggtgcgagac gggcgtcctg
     7261 cgatgcagcc agcgggagcg cggtttccgc gacttcgacg aggccgagtt gcgggtggcg
     7321 gcgctggtcg cgcagctgcg gcgcaaaggg gtcgcgctga ccaggatccg ccagatgcgg
     7381 atccgaaacc cgcagggcga tttcctggtg gcgaacggcg cggcctattg gtggtgcacg
     7441 agagaggaac tcctgccgga actcgccaaa acccgcgggg cctgtgtggt ggtttccctg
     7501 aaaggcctat gaacggtacg ctcaagcaga ggagcttcca tgagtccccg gcctgccgat
     7561 cctcccctcg accgcgaatg gacgcaggaa gaactgcggt cctatctcga accgcacggc
     7621 ttcacgttcc gcgagggcga ggtgattcca caggggtgca agtacatctc gtttcagccg
     7681 gtgactgaga aggagattcg gtgggttgaa gaccttgctc ggcgattcga ttgggcgaag
     7741 cgattcggtt ggtagagtaa taagtgaccg actaggaaac gccggtgaag ctccggcggg
     7801 tccctatagg ggactctggt gtagagagga acgggatgcg cgctaacccc cgtgaacaca
     7861 cctagtggcg caacatgcgc gttgcgtgca ttttttcgga gaaatgatcc gggaaatgac
     7921 gaccgccaaa gacatccgcg tggccgcgat cagcagccag gccggcaacg cgctgatccg
     7981 caaactgcac tactcgcaca aggtggtggt gaactcccag ctgcacctgg gcgtgttcct
     8041 gggcacgcgc ctggaagggg ccatgcagtt cggcccttcg ctcgaccggc gcaaattgct
     8101 ggggctggtg gagggcacca gatggaacgg ctttctggaa ttgaaccggc tggccttcag
     8161 cgaaaacctg ccgcgcaatt ccgagagccg cgcgctgggc gtggccatgc ggttgatccg
     8221 ccgccagtat ccgtggatcg aatggatcgt ctctttcgcg gacgccacgc agtgcggcga
     8281 cggcaccatc taccgcgcgg ccggcttcgt gctgacgcgc atcaagcgca acacgacgct
     8341 gtgggccggg ccggatggaa cggtaatcag taacgtcggg gagcggacca gcatgaagaa
     8401 gattcaggcc accgcggcgc gcaccagcta tcacggtggc gccgacatgg accattacaa
     8461 ggcgatgggg ttccagccgc tcgccggata ccagttgcga tatatttact tcacgaatcg
     8521 cgcggcccgt gcgcggctga cagttccgat tctcccttac actgaaattc accgtgcggg
     8581 ggccgcgatg gttcgcggcc agcgcgtcgg tagtattccg gctgaaacaa cggtgttcca
     8641 caccgaagag ggcggttcga ccccgacccc gacgctccag caacccacct cgtgagagat
     8701 tggtaatcgc cctatgaccc tcaacattgg cttttccacg cccctggtag tccttcacaa
     8761 gccgtggtct gatgaatcct ggggcgcagt cttcacgttt cagataggca atttcgtttt
     8821 caaaggagag cacatggaag ctacgatgca agtcggcgcc cgcgcatccg tctcggtcga
     8881 atggaaggac acgggcggca atatcgtcaa agtagacggc cccaccaaat gggaatcgtc
     8941 cgcttctgac atggtgcagg taaccccgtc caccggcaat ccgcagatcg ccaatctatt
     9001 cgcgccaggc cccatcggca cggcgcagat ccacgccacc gcagacgccg acctgggcgc
     9061 cggcgtcaaa ccggtcaccg caacgattga tgtgaccgta atttccggtg aagccgtcgg
     9121 cggcgacatc accttcacgc ccacgcccag cgcagcggcc caacagccca agcggcggta
     9181 accaaaaaaa gcgcccccag ttagagggcg cggagtttgc tcttccaaaa gaaaattggc
     9241 tttcagttta gcgcacccct ctattgtccc gccgccgcac atttgagcgg ctcccgttaa
     9301 agaattcctc tttttcgacc ggaaatgagg ctgcaaccca gcccatccga gcacctgcac
     9361 ataacatcct ggctgtttta aaataaggat atgaaaacaa aacagccttt tctacgtaag
     9421 gataggcaaa aaaagaactt ctcaatcatg gatctactca aagcggagaa catccggact
     9481 aacgctgacc tcgcagcttg gtgcatcaag aacgggcgga agggcgagtc cagatattgt
     9541 gtcctggttc ggcagttcgg ggaagtaaac gcggatcgga tcgtgcgcct tctggaggtt
     9601 gcggagtggg acctgaggga ggtcaattag ccatgacgag acgatttgtg gcacccggcg
     9661 atgtggtgcg gctggagatc ccctcttctg aggtctgcat gtccatgcat gtggcgggcg
     9721 aggtgatgaa cgtgcggttc caatcgccgc acagctatcc caacgcccag ttgctcatgg
     9781 acgatggccg cgagttcagc gcgccgatca cacctggcga ggcggggttc tactacgaag
     9841 acgggaagta ttactgctac ccggtcgatg cgcagaacaa cggggggcgg ctatgaaacc
     9901 ccccgatctc actctgattc ctgacgccgc gctgctctcc gaagtgcagc ggcgcaggaa
     9961 cgccaagcgg aaaacgttcg gcgcgggccc gggccggccg aaggtgataa agacctgcga
    10021 ggcctgcggc ggcatctttt ccgcccgcga gttcagacag catcggtgca cgggaacagg
    10081 aaggatcaac caatgagcac acccataatc atcgtcatcg gtgatgtcgg aacgggcggc
    10141 tggtggtacg ccatcgagga acagcaaatc ttcggggacg gcttccccac ggagaccgcc
    10201 gctatcgccg cggcggccac cgccgccgga gccgcaccgt acgagatctg gtatcggtgc
    10261 gaggctgagg gccgcacagt gatggtgccg ggcgcgcgat ccgccaatca cccagaggaa
    10321 ggcttatgag cccggatccg aagcggatag ccgcactgaa tgatctggcg cgcaccgcga
    10381 tgggggtttg cagccgggta ctgcaaactc gcggcatctg cgcgctgccg ccagcggacc
    10441 aatcccgcat ccgggagaag gtggaattgt tcaacgactt ccatcccggc gacgatccct
    10501 acggggagca cgatttcggc tccttcgagc acaacggggt cgggatcttc tggaagatcg
    10561 actactacga cacgaagctg gagttcggca gcgaagatcc gagcgatccc gcccagacca
    10621 cgcgggtgct gaccatcatg ctgcgggagg aatactgatg cagcgaggca ctcaggtatt
    10681 tgtgaccggc gctaaacgtt ttaccggcac cgtgctggcg gcgggtaagg acagtctggt
    10741 gatcgaggtc gctccgggcg tcagcgccac ccttcgccgc gatatcgcca gtaccaagcc
    10801 attgcagtgg cggtgcgccg gccttccggt ggaggtttct gcaaaacccg ctaataatat
    10861 aagcggcgac tgcaaaccgg cctcaatcga ggggccaaaa cgtgtagtca atctgaagga
    10921 gtttcgcaag cggctattcg cgctggaaaa gcgaacccgc aagttgtagt atctatccca
    10981 acgccccgcc cggtttcggc gggcgttcgg gtctgcactg taaggtccct tccccttttc
    11041 cctcaactac atgccaagtc ctgttgacac agcgccagct atgccgtagt atgcgcccat
    11101 gaagcgcaag aaagcaggga aagcggggac agccgccgac ccttttgaca agaccaaacg
    11161 ggatgcggcc gagtttctcg gcgtgacagt ctcgaccatc gaccgctacg tgaggaagcg
    11221 gaaagtgcca catatcaaac tgaacaacca tttggtgcgg ttccggattt ccgacctggt
    11281 caagttcgca gaaaaacaaa gggtggctta gatggcgagg agcatcctac gggtccgcga
    11341 gggcggcgaa agctgtccct ggtgcgccag caccggcagg tcgccaaaag gtgacggtcc
    11401 ttgcccgctc tgcgctcgcg ggcgcacggt cgggagagtg ccgtgaacat ctgccaatac
    11461 tgcggcgagc cgatcctgcc ggaggaagag ggccactctc cccgcgttcc cggtcaccat
    11521 cgggaatgcg cgtttcgcgc cgttggcggc tcggtcgctc acatcgagcg ccggtgctcc
    11581 tgttttgtgc cgggctcgac cgaaaacgat ccgccgggca tgacactccg gcaggccgcc
    11641 gaggccgcga taaccgtcgc atggaatcgg aggcacttgc aatgaccaaa ccaattgaca
    11701 ttttcccgac gccggtgttg tccgaggatg aagggcagct taccctgttg ttccctggca
    11761 acgtcctggg cgtttcgctg atgaccgaca gcgccctgga ggagcgcgtc acacaggccc
    11821 tgattcgcgc ctggaactcc tccctgctcg cgttccagcg ggaggcctgg aatatcttca
    11881 ccagcggcac gcgcgtgcca ggcgcagggt ggctccggcg ttccgaggac gtttccgaat
    11941 gactaagttg gctatgaagg tgcggcaatg cccctattaa acgcggaaca aatcgcggag
    12001 ctactgatga tcgcggagca cagccgcaac tgcatggagt gcggtcaccg cacggcacaa
    12061 gattactgcc gttcgtgcga tgaattttat tggcttcaca agccggggtg cgggatgttt
    12121 gaaaaccact gcggccatag actcaccatc gtgccgttcg tggaggtgcg gggtgcataa
    12181 acaggggcca taccgggacg gcaagattca cgtgaatcaa agaatgtgcg agacgtgcat
    12241 ttttcgcccg ggaaatctga tgcaactgga gccagggcgg cgggaatcgc tggagcggca
    12301 ggcggtgcgg cgggatggct gtatcccctg ccatgaaacc ctggacggtc cccgggtgat
    12361 ttgccggggg ttttttgatc ggcacaagga tgacgtggtg ggcctccggc tggcccaggt
    12421 atttaagaag ttggagttcg tatgacagag acccatctac tcactgatgc cgaactgcaa
    12481 ggcatcgcgt tgcaggcgct gaacatggcg aagcgcgact tactccagca caaattcaat
    12541 ttcctggtcg gcgcctatca ccacggcgaa ggcctgcacc gaatgagaaa aatcgagacg
    12601 ctgctgatcg agacgctggg ggaggactgg ctcaacggag gccgcaccaa agatgccggt
    12661 ttcggtgtgc tgcggtcggc ggtcgagcgg atgcctcccg atgcggtcgt atttgtcagc
    12721 atcgtcaatc aatttatggc cgccgaaaag ctgcggacgc tgggcgagga agaggcccgg
    12781 gagctgctga actctaccca cgatcggcac caccgggcgg tggccgaggg cctgctgacc
    12841 gtccacgacg cgctctacgc catcgcgcaa gacccgcggc gcatttgcat gtacgtgcag
    12901 atgatcgacc ggcacctcgg gtttctcggt ccgccggaca tccagtttat ggaccaggac
    12961 aacttcagcg ggcgcatgaa aatgtacggc cccaagtccg gctacacgga cgcggtgcgg
    13021 cccgcgcgaa aggccgccca ataatgaggg aagaggttgc caatgctctg gatagcgacc
    13081 atgtactgcg aaaacggcca ccccatcatc ggcgcggtat gcgtgctgga gcgcgccgga
    13141 cttcccgacg agcgggagtt ggatgagttc gttcaatcgg cgcgggcgtt ccttgccgag
    13201 gctgcggcgc agcagcgcgg caccgcgctc tgtgaggact gcggggcggc atcgacggcg
    13261 ctacgtctgg aacaaatcga cgatcccgat ttcgagaaga ccgttcatga gttcgcggcg
    13321 gatctgaaaa agccgtccgc caaacgaaga gaggagcagt tatggaagct gcggaattga
    13381 agctggagtt gatcgcatga gcgtcatgac gcgcgtcgaa cacctggctt gggcgaaaca
    13441 gcgcgccttg cagtacctca atgcggggga tttgatgaac gcccttgcgt caatgggttc
    13501 ggacctgtcg aagcatgagg agctaagcaa gatcggagac atgctgtttc ccctcggtat
    13561 gctgtacgtg caaaaccacg atgttggcgg aatgcgccgc tggattgaag gtttcaacta
    13621 gccatgacct atgaggagag gttccgtggg ctggcagacg cggcccgcat gctccgcgtc
    13681 atgatggagg cccgcgaggc tgcggccaag accgcgcggt tccggccaga cggcaaatac
    13741 aagccgcgca tttcgcgcaa ccgcgaaata gcggataggg ctttgctgcg ggagccgatt
    13801 cccggcggcg gcggcgccga gtggtggatc gcctacggcg ggatgcgcga gaacattctc
    13861 gaactgcgca agagtccgat attgccgctg gtcgaaagaa ctctcgatcc cgaagcgctc
    13921 aacggctacg tagcgttccc taaacgtcac gcacccaagc tgccgaccaa ttcgtatggc
    13981 ggcatcttgc agtacatccc ggtccacggc ggcgtgacct acgccgtcaa ggactcctac
    14041 atggccgtct ggggcttcga tacgctgcat tgcgatagcg gcgaggtgcc gcgcgccgac
    14101 cgggcgtgga ttctcgggca atgccggttg ctgcacaccg gactcctgat tgccgccaag
    14161 ctgtggccgg agtggcgtaa ggcgagccag gcgcgccgca ccgagatggc ggactggatg
    14221 ctccagttgg tcgacgatgg gcgcgtgggc ctcactggcc gcctgaattt gggcagcatg
    14281 ctcgacctga tgcgcgggag gatcggagag tgacgaaggc aacgaagaaa gccgagttca
    14341 agggacgatt ggaaatgctc gacccgcttt gggtgcagat cctcgaagcc aagccgaacc
    14401 cgctgtttgg cgaggacgcg catgaatggg ttcgcttcct aggccggagc atgaaaatcc
    14461 ggctggccat tcgcggatac gtcggcaagc gggtgatgtt cgaggaccgg gtggagatgg
    14521 aggaggccgc gctcgaccgc ttgctgccgc gactggccga gaaacacgcc ctggcactgg
    14581 cggaacacgc gctgcacatg atcgagatcg agttcctgga tgaggccaat ccgctcgaac
    14641 ggttctttcg gttcggcacg gacccggcgg gaatggtcct gcccatcgag gtaaagctgt
    14701 gaccgaaggc gtatattctg gtctgatgac gttcggggtg gatttgaaat ggctcaccgt
    14761 gtcggataaa gagtctcggt actttgcggt cgcgttcgac taacaggcaa aataggtgaa
    14821 atgaaatgtt ttctggacct gctttcccgt atcgtagcta tccatacgaa ggtgcattgc
    14881 acgaacactt gaccaacctt caaagcgatc ggatcggtat cgactgcgat aactacggcg
    14941 tatttatctg gtggccgcaa gattactttt atacctacta tcagttatct ggcggcgtca
    15001 ctgaggagga ttggttgact ggggtgcatg agttcacgtt gtccgaaatc gacgcgaaat
    15061 gcagtcggag atcccgaacc cctggcggaa aagaaactga accacatgag catgagcctc
    15121 agcagtgagc aatacatccg cttcggattc tacgtcgggg cagcgggcct ctggctcggc
    15181 gattcgagct atgagaaacg ttggcgcggc aaggtcgagg aactgaccga ggagttgtat
    15241 tgggaaatcg tcagcgagat acaacagaga ttggtggccg ccggtaagtg ccgcgcggac
    15301 gaagacattt tcgagaccag tttgtcggag gcaacctagt atgtccccgt tactcgatcc
    15361 tgcccatccg ctcgcgccga aattctggcg caacgaaacg ggcggtgaac tccagccggc
    15421 agtgatggcc tatctgaccg gcgagccaat gaccctgcgc cagatcgcgc tgatgcgggc
    15481 ttacctgcag cagtggatcg attcgccggt gtggaacccg cccgggccgc cggatgtacc
    15541 caagggcacc tgcaccgacg cgcggttgga actcgaattt atccgggcgg cgctcgcgga
    15601 ggtccgcacg cgggctcaga tcaacgagtg gctccactcg gcactaagca tcggcatcga
    15661 tccgctatga gcgacgtcta tctaagccag agccgcacgc cccttccgca tctgcgctgc
    15721 ccgaaatgcg gcgggccttg cgacggctgg agcggtctca gcatggagga gtttgctcaa
    15781 ccggagccgg gctcgcgcac gctctgtgga acctgtggcg cgtttttggt gttcatcgaa
    15841 agcgctgcga gctaccgcgc gctcgctctg aggatggcga ccgccgccga gattgccgcg
    15901 atctgcgcgc ataaggaccc caaggtccgg caactctgga cgctcgcgca ggagttcgtt
    15961 gcgaagcgca accagcggca gcgcaccggc cagggatagg cacctgcaca taacatcccg
    16021 gctgtttaat aatggccgca tgcaatcaca aaagaagccc gctaagggca aagcagagca
    16081 ctaccagatc ggcacagtta cctgcgctcc tcttgagcag gatctcgttc gggatttcct
    16141 attggcggcc gagaagttga cagccgatga tcccagccaa agggaagagg tcgaggaaat
    16201 cagggcgcgg gcctccaagc gcgggaagtc cggctattac acttccaagg ggaaaaaaca
    16261 gaggaaggaa gatcggaagg cgttgtggca gattctctgg aattgcagcg ggccgtactg
    16321 cactttcgat gagcatcccg atgatccggg gaattggggg ttctggctta tctcccattg
    16381 gcgcgaagag ttgcttgagg ggcatggaat cattgtttac gaggtgcggc agataccgaa
    16441 gaacttcgag ggcgatttct tgctcgtgac gaaatcgggc aacctgattc ggaaggtggc
    16501 catgtgctat cgcggcgcgg ctcctgccca tgagatgcag actttttggg agatggaggg
    16561 ttcgctgatc ccgaggtcgt atctgcccca attgcagtaa gccgcgtccg aatccccctc
    16621 agcaaagccg cccgttcgaa ggcggctttt tggtctgcgc cgttcgccgc tgaagtgaga
    16681 agttcctgct gataagtcac gtggaatcac caatttgcag agttactagc gcgactgtgc
    16741 gggcggcgag cccgcgggcg gccagacgcg gcggagtacc tttaactcta tgattctaaa
    16801 gggtctaggc gattcgcgcg cacgtagcgt tggcgtgttg ataacaaagg ggttcgctct
    16861 gtgggggcta tgggctatgg cctatggcct atgggtacgg gcggctgggc ggctgtgggg
    16921 cggtacgggc ggctgggcag ccttgggagc ggctgggcgg ctgaaatggg gcggctgggc
    16981 ggctgtaggg gatacgaggc gattctaggg cagctagcgg gcggctgtag gggattgtac
    17041 ggggcggtac gggcggctgg gcggctgtag agcaaaaaaa agccgccccg tagggcggcc
    17101 cggctgggcg gctgttttag gcgaatttga attcaaatcc cgcgctaccg tagggctttg
    17161 cttcgatgtc atacggcctt gagccatcga atcccaatgt tttgaattgc ttggtagcgg
    17221 agatgtacgg cctacccgaa gcatcgcgca catccagcgc gccttctacc tctttcgctt
    17281 gaggcacaat ccgtaccatt cgcccttcga tggtaagcgt agcgaatttg ggctttggcg
    17341 tacctagccg ctctgctgca tccttcccga agcatacgcg ccctgtcttg ttgagatgaa
    17401 tcgcaaattc tgtaggcttg tcggccttgg gagcggcggg agcggcggga gcgggcttgg
    17461 cagcggcagc gggcttgaca gcagcagcgg gcttgacagc agcagcgggc ttggcagcag
    17521 cagcgggctt gacagcagca gcgggcttga cagcggcagc gggcttggca gcgcttttca
    17581 gggcagtagc taaggccttg cctacggtag cggtaacatt caaattcttt tttgccattt
    17641 ggagtaaatt cccttatttt tgttcgattt agaggcggca tctccgccac aatccgaacg
    17701 tagcatgttc ccacaaaaaa agggaagcgc cgtggtgctg cacctactaa agccttatgc
    17761 tacaatgatt tacatggttc ggatggcaga gccagaaagc gcggcaggta tagacatagc
    17821 caaccccacg ccatagccta agccgtgggc caacggtacg tggtacaggg gtacgtggta
    17881 ccccccaacg gtaccgccac gggggtttct atgccagaag acgaaaactc gatgctgggt
    17941 cgagttgtta ccgtccactt caccgactcg ccgtccactt cacgatttga aagttaggct
    18001 ctccctttcc gctgccgtga ggatctcccg gcggtatgct ggtgcgatgc caatcactga
    18061 agaaaggagg gcgcggaacg tagccagagc aatcgtccgg gcacggccca tacggagcgc
    18121 acttcatctg gccgatgcag tgttgcgggc cgtgccgagg acggaacgcc tgagccgcat
    18181 gggcctgatg gagcgttttc tgctggtggc cgccgcattc gtggccggcc tgctgatccg
    18241 agcggtgcta tgatctgccc cgagtgcggc atcgaggtga aacgccatgt ctgccgcgcc
    18301 tgccaagcca agcgcactcg cgcggggatg ctgcggcacc agcggcgctt cctgcaaacg
    18361 tggctggccg gtcagatcga attgcgcgtg aagcgcgtcg caggcgtgct gcacttggag
    18421 ctattcgacg accgctggca cagctactgt gacaccgaga tgttctccgt tcaccagcgc
    18481 gatttcgtgc gcgaactccc ggccgacctg tgtccggcgt gcattaccat cttccatgaa
    18541 caggtggccg cagccaagga ggtccgatga ttaagctcaa actcctgctc atgattctgg
    18601 cggtgctctg ctttgctctg ggcgcatctg aagtcaagcc gccgcgcggt aatttcgtgg
    18661 ccgcgggcct gtgcctgtgg gctctggcga cggtcgtggc aggctagcgg tgctatgctc
    18721 gcggagtaac actttgtttt ctgccatgct taaacagccc gcgcttcctg ccaactggtg
    18781 agcgtgggct ttgtttgttt ttcgcaggat ctcccgtgac aagagcgact tccgggttta
    18841 gaatggccgc aggccgtcgc cgggactagg ccttgaaagc aagtgataac atcccgcacc
    18901 tccgagggat ggaatcacaa tgcccctggt caccgaactc gagctggcgc gccaccttaa
    18961 aagagaccgc aaagctgtgc gcctggccgc gcaaagcggc cggctgacga ctcgagcaga
    19021 cggtctgttc gatctcgacg tagcggtcag ggaatgggcc gagaccactc accacgagaa
    19081 gggtcacaac aaccgcagcc tcgccgagat tccgtcgccc gacattccac tgcgacctga
    19141 aaccaagagc accgactatg ccaaggcgcg ggccgggacg cagatttacg aagccctgct
    19201 gaagaaactg cgttacgagg agcgcgccaa gaacctgact ccaaccgcgg atgtagaaac
    19261 cgcgcgtttc accgagttcc gcatcctgcg tgaggcttgc ttcaacattc cggcgcggat
    19321 cgcggcgctg atcgccgggg agacgcaagt ggagcggtgc cagcagctgc tcgaaagcga
    19381 actgaacagc gtgttcaacg ccttcgccga cggcaggctc tcatgacgcc cgcactgacg
    19441 ctgcacgctt cgctgcccgg tctggccgac gcctacgaag tcagccggca ggcctgccgt
    19501 gccggggcgc ggccggaccc caagctgcgc atctcggagt gggcagacca ataccgggtt
    19561 ctcacgacac gctcgtcgcc ggagcccggg atgtggcgca ctgagcgcac tccatttctg
    19621 cgcgacatca tggactcctt gatgcccgag gctccgtggg agcgggtggt gttcatgaag
    19681 gggtcgcagg tcggcgcaac ggaatcgggc aacaactgga tcgggtacgt gatccatctc
    19741 gcgccaggcc cgatgctggt ggtgcagccg accgagatga tggccaagcg caactcgaaa
    19801 cagcgcattg gtccgctgat cgaggactcg ccggtcctgc gcaccctggt acggacggcg
    19861 cggtcgcgcg actcgggcaa cacgattctg gccaaagagt ttctgggcgg catcctggtg
    19921 ctggccggcg cgaacagtgc caagcagctg cgttcgatgg ccgcgcgcta tctgttcctg
    19981 gacgaggtgg acgcctatcc gcccaacgtg gaccgcgagg gcgagccctg cgatctggcg
    20041 attgcgcgca ccagcaactt ccggcgcaag aagattttca tcacctcgac gcccacgatt
    20101 gcgggccgca gccgcatctg caccttcttc gagcagagcg accagaattt ctatttcgtg
    20161 ccctgcccgc gctgcggcgt tttgatcacc ttactgcccg agcagctggg ctggtcgccc
    20221 accctgaagc atcaggccag ctaccggtgt caggagtgcg agggcgagat cttcgatcac
    20281 gagaaaacgg ggatgctgga gttgggcgaa tggcgcgcga ccgccgcggg cgacggcgtg
    20341 acgcgcggct accacttgtc cagctattac tcgccggtgg gctggctgtc ctggacgcag
    20401 atcatgcgca accacgacaa gagcacggcg tctcccgaga agttgcaggc gttctacaac
    20461 accgtgctcg gcctgccgtg ggcggaccag ggcgaagtgc cggacgtgga ccggctctac
    20521 gagcgcaggg aatcctacgt gatcggcgaa gtgcccgagg gcgggctggt gctgaccgca
    20581 ggcgccgacg tgcagctcaa ccgcatcgag gtcgagatcg tggcctgggg ccgcgaccgc
    20641 atttcctgga gtgtcgacta tcgcgtgctc gaaggcaaca gcaaccagcc ccaggtgtgg
    20701 gagcagctgg cccggctgat ggacgaagag ttccccacct tctacggcgg cgcgctgcga
    20761 attcagaaac tagccgtcga ttccggtttc aacaccatgc gggtgtatga gtgggtgcgc
    20821 acgatgctcg cgcagcgcgt gatggcggtc aaaggcgaga gccacgtgcc cgcattggtc
    20881 ggctctccga acctgatcga acgcggcccg gccggacgcc tgattcgagg cggcgtgcgc
    20941 ctgtggccgg tcaattcctc tatcggcaag gaagaactgt accgctgcct gaaactgtcg
    21001 gccccagacc tcgcggccgg cgaagagtgg ccagcgggct attgccactt cccgtcctac
    21061 tctaaggagt ttttcgagca gctgtgcggc gagcagctga ttacgcgcac gctggcgggc
    21121 cgcaccacca gccgttggga aaagcggcgc gaccgcaacg aagcgctgga cgtcaggatc
    21181 tatgcgcgag ccgcagccgc cactctgcgc atggagacgt ggcagccgaa gcggtggaac
    21241 gacatcgaga ccgcgctgcg cggcggcagg gccatcggcg gcagcgtcgc caaaggcgtg
    21301 cagtccccga ttccgcagtt tcgcccgctc agagccaacg aaggatttct ggaatgacac
    21361 cgaacggcag gcttcccttc caacccaagc cgatggccgc gaacgtccgc ttcaagctgc
    21421 ccaaggtgac cgatccggcg actgccgcca ctatgctgga gcaggcgcag caggcgatgt
    21481 tcaacctggt caccgggcag ctgccgagcg gcgtcgaaac cccgcagctg ggccgggtga
    21541 ccttcaacgc caccaacacc gccgatttgc agcggctgat tgattacctg agcggggtgg
    21601 tcgccagcgg tgacggcagc ggcaacacca accggcgcat cccgattagt ttcctggctt
    21661 ggccatgaca gcgacgcccg aatccaaccc gacgagctcc accacggtcc ccgcgatccg
    21721 caagccgggg ctattgaagc gtctcttcgg cggcgaccgg agcgcggccc aattccaggc
    21781 gcagtcgccg gacggcggtt ccaactacgg ggcgggcacc ggctacggcg cactgggcgg
    21841 ccgcgacacg ccctataccg gcgcgtcctg gattcgcaag cagatgtcca actggtttcc
    21901 cacgcgcgcc tcagccgacg ccgaactgtt gggcgacatg ggcacgctgg tggcccggtc
    21961 gcgcgatctc gaccgcaaca ccggcatcgc cgcaggcgcg atccagacca ccgtggataa
    22021 cgtggtcggc gccaatctcc gtctgtcctg ctggcccgac taccgggctt tgggccgcga
    22081 cccacagtgg gccgaaagct ggggcaggag tgtggaaagc ctctggaaaa gcgtggcgga
    22141 taccacggct ttcgatgttg cgaacaagct cacctttacc gggttgacta ccctggtctt
    22201 ccggtcggtg ttgcagaacg gggaagctct ggcgctggcc ttgtggatgg cgcgggccga
    22261 cgcgaaattc aagacctgcg tgcagctggt cgataccgac cggctctcta atccggggaa
    22321 catgacaccc acgctgaccc tacgcggcgg cgtggagacc aacgattacg gccgtccgct
    22381 ggcctaccac gtccgcaaga tttccaactg gcccgccatg ttctttcccg ccatcggcgg
    22441 tatcggcggc gagtgggaac gcatcccggc ggccaccgaa tggggccgca agcgcgtgct
    22501 gcacgtctac acccaggacc gcgtggacca gacgcgcggc aagccaatcc tggctccggt
    22561 gatcgagcag ttccgcatgc tcgactccta catgcgcgcg gagctgcaat cggcgattgt
    22621 gaacgccctg gtggcgggcg tgattgaaac cccgctcgac cccggcacgc tggccgagat
    22681 ggtgggcggg gatgccaacg gctacctggc cgccaagaac gagtaccgca tccagctgga
    22741 agccggcacc ttcattccgc tgtatcccgg cgacaagatg acgcccttca tgcctgcgcg
    22801 gcccgcgcct cagttcgcgc agttctcgga attcgtcatg cgccagatcg gcgtcagcat
    22861 gggcctgccg tacgagcagc tgatgaagga ttactcgaag accaactatt cgagtgcccg
    22921 cgcggcgttg ctcgaatcct ggcgctattt caccacccgg cggtcctggc tgtgcaccta
    22981 ctgggcgcag ccattgtacg aattgtggtt cgaggaagcc gtcaacgagg gcctcatcga
    23041 cgcgcccgac ttctacaacc agcggaccct gtacacgcgc gccaaatgga tcggaccagg
    23101 ccggggatgg atcgacccgg tgaaggaagc tgaggccgcc caagtccgca tgtcgagcgg
    23161 catttccaca ctcgaagctg agtgcgccga gcaaggcctg gactacaacg acgtgatcga
    23221 ccagcggcag atcgagaagc aacgcctcca ggaagcgggg ttgtgggtgg agccggcgcc
    23281 gccgaccaaa cccttgggat tcccagcgca accggaagag acgccggtta gagagcaggt
    23341 ataagttatg gctacagacg aactccttac cggcgaagca ccgctggcgt tccagctgct
    23401 atccctcaac gaccgcccct gggccatgag cctcacggcc ctacggtcgc tggtggcggc
    23461 ctgcggcgcg ctcgacttcg aggctgtggc cgcgcgcgcg ggacagccgc tcgataatgg
    23521 cagcggggtg atgaattacc acggcaccgc ggtagtcaac attcgcggtc ctttgttccg
    23581 ctatcgctcg atttggacgt ggctcctggg cggcacctcc gtcgagcaga cggcgctggc
    23641 cctgcatgca gctgtggacg accccacagt gcagcgcatc gtgctggcca tcaattcgcc
    23701 tggcggccag atcgacggta tcaatgaact ggccaacatg atccgcacgg ccaaccagac
    23761 caaacccatc accgcgtacg tggatggcca gggggcgtcg gccgcgtact ggctggcttc
    23821 agcagctggc acaatcgtgg ccgacgaaac cgcgcgcctt ggctccatcg gcgtgcgggc
    23881 cactgtgttc gatgagcggg cggcggaaga gaagcagggc gtgaagcggt acgagatcat
    23941 ttccagccag agtccgctca agcggaccga ccccggcacc gaccaggggc gggcgcagct
    24001 gcaacagatg gtggacgcga tggcggcggt cttcatcgca aaggtggcgg agttccgggg
    24061 gaccagcgaa gagaaggtgg taagcgactt tggccaaggc gcggagctgt cggctcgcga
    24121 cgccgtctcg gtcgggatgg cggaagcgct cggttccctg gagggcctgc tgggcgctgg
    24181 caacggcgac ccagtgcggg tggtgcgcga tcagcccgcg ctgcgcgtcg gcctggggac
    24241 gaccgtgccg gccgccgtgc tgcatatagg ggcggcggcc acggagccgg aggcggcgga
    24301 ggagaccgac gacgaggaat gcgactgccc gccgggcgaa gactgcgaat gtgacgagga
    24361 agaacaggag ccgctcgata gctcggcttc agaaggagat ggagacttga ttacgccaac
    24421 cgaagaacgg cagcggatcg ctgcaatttt aacctgcgag gaagcgcgcg gccgcgaaga
    24481 gttggcccgc acgctggcac ttgaaaccaa ccacaccctg gaagccgccc agaaactctt
    24541 gaaggcgtgt ccgctggcgg tcgctaccaa gccggtcaat gcgctcgaag tccgcatggg
    24601 ccagatccaa aaccccaacg tgggagtaag cggcgatgcg gtcgccgacg attcgccggc
    24661 cgccgaggtg cagcgcatcc tggcgttcgt tccgaagtcc cgcaggcacg cggtgcagta
    24721 aggagaaact atggcaacac caagtgtgaa cccgattaac gttgcgtctt tccggtcgga
    24781 catcttcaac tacgactcgc tcctcgcggg cgagtgtctt tccaaaagcg ccaagatcgg
    24841 cgcaagtcag ggcatcctga aacgcggcac cgtcctcggc ggtgtggccg caggcgtgcc
    24901 gccaacgggc gttacgccgt tcgtctatgg caacgcgcca gcgggcgcga tcctggcggc
    24961 ggacatcgac accggcaccg gggccgccgt caccggcctg gtttatacgc agggaaaatt
    25021 cctggacact gccatgattt tctcggccaa cggagccgcc accgacgtag cgcagctgtg
    25081 gaacgtcggc atctacgtcc tgaccgtcga acagcgcagc ggccaactcg tgccgctgat
    25141 gaacctgccc acgtcgggcg caggcccgct gccgcaagcc atgtcagcga aagacgcagc
    25201 cgaagccacc aaggaggaag tggaggcgat ccgggccgcg caagggcaga gcccagtggc
    25261 gatccctggc ccggccagcg ccaaagaccc ggcgtggtat accgcagcct tcggggagcg
    25321 tgaactgacc aaggaggagc aggcccgcga gaaaaccgtg gagcaggttg ccgacctcga
    25381 cgagaaacag cagaaagcgc gcgaggagct gaccgccaag cagaacaagg aagcctccga
    25441 gttggcgaag aagcagcgcg acgagcgtgc ccagctggca aagcaggaag cagccgccat
    25501 gcagcacggc cgccaagcca acccaccggt gccgactacg gcaccgaaga aataaccccc
    25561 ctgcggcccg tcgtcttggc accagacagc gggtcgcctg atttctaacc cacccagtac
    25621 accgggaggc aagccaacat ggccgacgtt tttagtacag atgttctgac cgctgtgctc
    25681 caaagtttac tgggcaaccc tcagttcctg ctggaccgct tcttcggcat cacgcaggcc
    25741 gaggccagcg aacagatcca cttcgacgtg atgcagggca agcggcgcgt ttcgcccttc
    25801 gtctcgccgc tggtggaagg acaggtggtc gcttcgcagg gattcgtcac caacaccttc
    25861 acgccagcct acatcaagga caagcgggtg ttcgatatga accggccctt gaagcgcggg
    25921 ccaggcgagc agatcggcgg cacgatggtc cctgcggatc gcctgcgcgc gctgatcgcc
    25981 ttcgatatgc aggaccagct gaatatgctg cgccgccgtc tggaggtgat gtgcggcgaa
    26041 gtcctggcca gcggcaagag cacgatcagc ggcgacaagt accccacgca ggtagtggac
    26101 ttccagcgct cggccaccca ccagatcgtg gccgctccgc tgtggagcac ggccagcccg
    26161 ccgattctaa acaacctcca ggactgggcc cagatctgtc tcgaagacac cggcgtcttt
    26221 cctaatgacg tcatcatgac cgtggatgtt tggaaagtct tccgcgccga cccggcgatt
    26281 gtcaacgtgc tcaatatctt ccgccgcaac acggacaccc cgagtgtcat gccgatggcg
    26341 caagtgaccg agggcggcgt gctgatgggc aatatcgaag ggttcaacat ctgggtctat
    26401 tcgggctggt atgtggaccc ggcagacggc gtggagaaac ccatcatccc ggcgggcacg
    26461 gtgatcatgt gctctccgtc gctcgaaggg gtgcaggcct atggcgcgat ccgcgacgag
    26521 gagatcggct tgcagccggt gccgtattac gtcaagtcct ggatccagta cgaccccagc
    26581 gtgcgctacg tgatgctgca aagcgcaccg atcatggttc cgttccgtcc caacgcctcg
    26641 ctggccgcga aggtgcttta aatgcgggac ggcgaagatc cgcggtgcca gcggaaagcg
    26701 cctagcgcgc cttcaaatct ttactggggg tttgggggcg cgggcgcgct gacgaggagg
    26761 gtttgatgag cagctttatc aatccgttcc tctccgacta cctgccgaac ctgttctggc
    26821 cggcggaact ggccaccttc gggaggcagg tccaatacct gccgcaaggc gacgcggggc
    26881 aagcggtcgc ggtgaccatc ctgtggaaag agggcgcgag cgacgaggaa gtgtcgccgg
    26941 gccgctacag ccatatcgac ctgcagaacg cggatctgcc ggtcgcgccc gccctgggcg
    27001 acattgtgca gaaggacggc aaggaatacg acgtggtgcg ggtgaacgct ctcgcggtcg
    27061 gctactccac gatcgtgctg caagaagggg gaacactcta atggcgcaag gaatcctggc
    27121 tggtcccatc gccgccacgc aagcctcgat gaacattcag gtgaagcgca ccggcagggt
    27181 gcgcgcgccc aagctcgacg atccgcagct cgcggcgatt ggcttgaaga tggtcgcgga
    27241 acagaaagcg cggtgggcca gaggcatgga tgcggccggg cagaccgcga agaagctcac
    27301 cgtccgctac gccatcatca aacaggccgt gctgcataaa cggcccgtgc gcgacatgca
    27361 cctgaccggc gccacgatca agaatttcca gctgcgcaag gccacgctgg ggaccatccg
    27421 cgccgagaac accacgcgcc tggagcggtc gaaggcgcgg cggtgccagg gttacgacca
    27481 gatgatcggc tttgcgctca ccgacaccaa agtggtgttc gatgcggcgc agaacgaatt
    27541 tggccagtac gtgaacaagg cctggatccc gctcgacggc actaaccgcc gcccagggac
    27601 gctgaacatg ctgccgtgag aaccggatga tcaagctggc cgatctggtg aatgcgatgg
    27661 cgcaaacgtt gaccaacatc ccggaactgg tggcccagct ggcgcccacc gatccggtga
    27721 aggcgtatat cgacttcaat cccaccagca attccgtgga taacgccatc tatcagatgc
    27781 agccgggcca gctgctggtc atgtgggtgg agaccgcgct ggtgaccgaa gccatgagca
    27841 agtggagcca ccgcgtggag atttgcgtgc gcgccatgcg cgaccattcc gacctcgatc
    27901 tggtagacca gatcctgaac ggcgtcccgg tccccggtga cggccagatc tggcgcatgt
    27961 gtcctatcct cgcgggcgtg cttcccaccg acgtgttagg cattgcccgc cgcaccgata
    28021 ccgaaggcgt ggactacgcc gtaattctga ccgagacggc cgagacaggc gactggcctc
    28081 tgccttaaac ccttggaggt gattcgaccg atggcaacct taaccgctcc cgcgccgctg
    28141 gcaggaggac cgatctcctg cccggcgaat attcaggaga ccagaatcgc gttcggctac
    28201 aagccgcaga cggacgtgcc gacgatcaac gcgcttgccg acatgtgggc gctgaccaag
    28261 gtgaatccgg ccctgtcggt ggtcaatccg gtcaacgaaa ccaacgcgct cgatatcggc
    28321 aagggtaacg agttccccag ccagacgttc ccgtcgcatc aggacaccag cgtggctctc
    28381 gaaaagtacg tctcgagtga gttcctcgcc tggctgttct gtttcacaac cggccaagcc
    28441 accaagaccg tggccggaac cggcttcgcc tatgcggccg tgcccaacga tcccgcggtt
    28501 acctgcatca atctgccgtg cttcacctgg gacgagcaga tccggccttc cccgaattcg
    28561 gtagtagacc gggcgctgat cggctgtgtg gtgggcgact gggcgctgac catgtccagc
    28621 gggccggggc gcgccaactg ccgggtggcc tgcacgcttc ccgggtcggg gcgcgtgcag
    28681 agtcccggcc tgtcgccgct gcccgcgctc acgccggagc acttcctgaa cgcctcgggt
    28741 gctttcatta ccatcaacgg cattgattac gtgcttcagc agaccttcat gagcctggag
    28801 tttcgctaca acaacaacgt gcggctcgac accgggctat atcccggctc gggcacccaa
    28861 aacggtttcg gcgtgcgagg ccgcatggaa tacggcgtcc gcgaaatgag tttgaatttc
    28921 gtggcccgcg cgcagaaagg cagcatagag tacaacaact tgatcaatca ggtggaaggg
    28981 ccggtgagct tcggggtgaa gggggcgcta atcgacacca ccttctacca caatttcacg
    29041 atctcgatgc cgcgcaccat catgcaggcg gtggtcaatg gcgacgacgc caacatcgtc
    29101 accgtgcagt gcggggtcac ggcgctgcaa ccgaccgacg ggatcacgcc catcatcacc
    29161 atgagcgcca ccaccacgaa ggacggaatc ctggggctat gaggctagaa gggaaattcg
    29221 tcccaatgaa agtcgagggt cacgttcgcc gcctcaaagt agtgcgcttg aatgcgtacc
    29281 cgctttgcag tctgcaattg agcgatcaat tccgattgcc cggtccaggc cgcgttggtc
    29341 tggtcatcga atcggagcat cgtgaaattc tggaccggcc cttcatcgaa gcgaacgcgc
    29401 aaagggcaca ctatgccgta gcttttccaa tccccaatga actggccacg ctccagatgc
    29461 agggacaaga taaagaggcc cggtttctct tcgctcactc gcaatgtgcc atgctgcggc
    29521 ccgttgtaag ggaaaggaaa ctcgacaaga ttggtagacc gcagcgtggc gaaccgctgt
    29581 tcggtcccat ccagatcgtc gcgggcgccc ccgtgtttcc actgctccgg ttctggctgc
    29641 gcagcctctt gatccggttc cttggcagcg ggcagaggag cggcgggaac aggaaccata
    29701 gccgctcggc cggagttgta ggcagttggg cgctccagcc ggaaccagta acccgcgcct
    29761 gcggcgatca ggacaataaa tatgaatctg tgcataccag taccattgta ccggaatacg
    29821 ctaagtgcag ccggatgaag gggatacgcc gatgcaaaac gcaatctgca aaaatggaag
    29881 gcgcggtggg ttatttctcg cgccttcctg tgtgccattg gcaccctcgc cttgccgcac
    29941 cgcgccaaac ccagccatgg ctagccatgc ccagccgtgc cgagccttgc cacgccaggc
    30001 ccagccaagc cctaatggac ttcacaatta tagaggatga aatcgatgtt tgataccgct
    30061 gcaaccatta ccatcgggct gcgcgtggct ggcgcaggga agaccgacat taccgtgcgc
    30121 tggccttccg acgaggaatg ggccatgcac cgcaagcgca gcaagttgat gatgcgccag
    30181 atgggccggg gcgcttcgga aatggagacc gactccagcg ctgccgacca gaagatgtat
    30241 gaagccatca agctcaacgg cgcgcctccc ttgactgcgg gtgaagccac gaccattatc
    30301 caggccattg gcaagtgcga cgtgctgcgg gtggatctag gtgcggacga ggcggaggcc
    30361 gagctgcaaa tcctgacggg ccgggtgacg cacaccgtgc gcatcccgac cctcgacgag
    30421 gtgcgcaagc tgcaccgtac gacgcgctac ctaaacctac cgtacaactg ccaggaggtc
    30481 cgcaccagca tggaagcggc ggcggcgctg tgggatgcct gcggcgggaa agcggacggg
    30541 tacgccggcg cggtgccgat tctccacaag gattcggtga tccgccaggt cattatcgaa
    30601 atcgagcggg aaacagcggc gagttatggc gaagaaaatt tttagcgggc gggggttggc
    30661 cggctcaacc ttcgccgagg tttatcctcc accgaatgct ccgccgcgac caactgtgcc
    30721 ccggttctgg cgaatgcccc gaggcactga tgcgcgatcc tttcgcggac gccaactccc
    30781 cgccgtgcga cgaatgcccg ctggcgctgc tggacgaata tctagcgacc ccagcaggcc
    30841 ggttgatagg cctcaccatc gacctggatt tcgcaatgca agcgggggtg accgttagcc
    30901 tccaagacat cacctacccg gagtttttgt tgctccgctt cctgagcgag gagcggaacc
    30961 gcttccacgc ggaagcggcg gagagggcgg cgcgtggcca gtaacacgat ttacctccaa
    31021 gtcgatttca acagcgagac cgcgcagcag aacgtcaacg ccctcaatca gtcgattgcc
    31081 acgaccggcc ccaccgcaga gaaaagttcc aaacaggcca ccgctggctt ccagtccatc
    31141 ggcgtctcga ttcagagcgt caaccgggag ttctcgcagc tgacctctgc cctggcagga
    31201 ctggggatcg cgcgcgtgat cgccggcatg gtgcagatgt ctgccgagtt gagccgcacg
    31261 cagttggcta tgcagtcgtt cacggggagc gcggcagagg ctaacaaggt cttcgagcag
    31321 gtgcgcgcga tcgcggcgac cagcccgttc cggttcaagg atctggaaga gacggcgcgc
    31381 cagttgctcg gcttcggcat ggccgccaaa aacgtgcccg acacgctgcg cgtgatcacc
    31441 gaccaggtgg gccgcatggg cggctcgatt gagaacgtca acaacgtggt gcgcctcttc
    31501 ggtcgcatca tggaaaagga tttcgtcgga gcaatggacc tgatgcgggc gctgccgcag
    31561 cagggcgtgc aggtgatgac cgcgctgcgc gaggccatgt cgaagcagct gggccgcgcc
    31621 atcgacgtgg agggagttcg ggacgcgatt aaggaaggca ttctggaccc gctgcaaacc
    31681 atccgcgtgg tgctcgactc catgcggaag gccggcggct tcggtgcttc gctggccgac
    31741 gccgcgctgg cgttcaagaa tatggccgac gcggctcaga acgcggtcag ccagctgttc
    31801 gggccacagg gctttggacc ggcgcttagg aagttgggca ctgagatcgg caacctgctg
    31861 gcgccgctcg gcggtctgct cgactatctg atgaaactgc cggatcccac gaagctggtg
    31921 atcctccaag tcttggctgg ggcggcggcg gtcgcggccc ttggcactgc gctcgccatt
    31981 gtcactagtc tagccagccc gctgctcagt ctcaccgcgg cgattttcaa gttcacggtg
    32041 ggactggcga ccatgaaccc gcaactggcc attacggtgg tgttgctcgg cacgttggcc
    32101 tttgccgcct ataagctgat cccgcaattc caagggatgg tggataccct ggtcggcggg
    32161 gtggccggca agattaaaac ggccctgacc ggcctggcgg aacagggcaa gaagttcatg
    32221 gccgggattt tcaagggcgg gggccagatc gaaccgggcg atatcctcga caccgccaag
    32281 ctgttcgaga aactccgtct ggaggaacag aaacacgctg atgcggcgca gcgcagttta
    32341 ctcgaagcgc tggcctcgcc ggtcgaggcg gtgcgcctga aatacgcgac cctcttcagc
    32401 gaactggagg aaaagatcaa actccttccg caggcccagc agcaggcact gcgcgccatc
    32461 ctggtcggtg ccgaagtgac cgagacggcg ggggcgaaat tcaaggagca gaaaaagcag
    32521 gaggacgaat tcacccgcta tcaggtggag cgcgccaagg gagcggccga cgcgcaggtg
    32581 gcctacatcg aggcgcagga cgctcaggat ctgcgcagca aggtgcgcaa catcgaccgc
    32641 atcacccaga tccgcatgga cgccgcgaag caggttgcgg acatcgaaaa caacaacctg
    32701 gaggatcaat tccaccgctt cgagaagatg gccaacgact accgcaagca gttcgccgca
    32761 gcgggcgtgg acgtggacaa gatgatcgag gcccaccgca aagaaaccga cgataaggaa
    32821 gcggcggtga ccaggaagca attcgacgag gggcagaaat accggctgga agggtggaag
    32881 aaggctaacg atctgatcat cgaagaccag aagcgggtgt acgacgcctt caaggatcag
    32941 ttcagccaac tgttcgacgt gttcaccgat aagaccaaga gcctcggtca ggcactgggg
    33001 gacttcttca aaaaactggt gctgggcgag gccaaggagc tattcgccag ccaactggca
    33061 ggggtggcca ccgcagcggc aggttatggg cagccgcagg aaagcttggg caggggtggg
    33121 caggggatcc tgggaaccct gctgcgtcgc gggatgccgc cgcggccacc cctgccgccg
    33181 ccagacgtgc agcagatcaa cgcgacgcag agcgacatct cgttcccggt agccagcgcg
    33241 tttcagaaca gcacgcacag ctacgagatc gctaccaacg cctactccga cgccaccatc
    33301 cgtttcgcgg aggcggtcaa caacttcgcc ggcgccaccg tgcagcatga gggcagcgcc
    33361 gaccgcatgg cggataccac caccaccatg aaggaggcct ttcagacagc cagcggggcc
    33421 accggcgtgc cgcagactct cttgcgggcc gtggcgcaag ccgagtccag tatgagaccg
    33481 ggggccgtct cgtccgcagg cgcaatgggc ctcatgcagt tgatgcccgg gacggcccgc
    33541 gaacacggcg tgaccgacgt gttcaatgtc gggcaaaacg tgatgggcgg cgcacaacac
    33601 ctgcaggaga tgctcacccg ctaccacggc aatgtgccgc tggcgctggc ggcttacaac
    33661 atgggcgcgc acgctttaga ccaacggttg gcttccggtc gtgcgctgcc cgcggagact
    33721 aaggcctacg tcgcccgcat cacgcagctg atggcagcgg cacccgaagc ggctggtggc
    33781 ggtggcgccg atctccttcc tggcggtgag gacgcccgct atctcgcggc cactggcggc
    33841 gggggcgctc ccgcggttac gatggctcaa ctggcagcgt tgccgcagcg cgcccagcca
    33901 ctgtttctgc caggtgtgcc cagctttcag gcggaaggcg cgggagccgt caccgggccc
    33961 agcgtggcgg aactggccaa cctgccgatg ggtctcacgg cactgcaaag gttgagtccg
    34021 tccctcttgc ccctgctaac cggcgcgttg aaggcgggag gagccaccac gggcgcgggc
    34081 ggagtgaatc tggcgggtct gaacatacag aatctctcga agctcgggca gttcttcggc
    34141 atctacggca agggagtcac gtcggccggc agcatcctga cctccccggc agcgggcacg
    34201 ctggcgctaa tggcaggcat gggactggcc agcaaagggt tgcagcagca ctacgcaccc
    34261 gcgacggtgg cgggcggcgc gctggcgggc gcggggatgt ttctgaccaa tccgagtatg
    34321 atcgcgcgcg ccagtgagat gcccggcggg gtcggccttg gcatcggcgc ggcggcggcg
    34381 accggcgcag gcctggggtt gttcgccagc gggttccagc gcggcggcgg cactggcctc
    34441 gcaatggata tcggcggcgg cgcactggcg ggcgcgggca tcggcaccat gatcctgccc
    34501 ggactcggca ccgccatcgg cgcggctatc ggagccgggg tgggcgcgat cacaggcatt
    34561 gtgcgcctgt tcgtgcatac cgagcaagag cggatccgca ccggcatcaa gcaggtctac
    34621 ggcatcgaca tctccaaccg ccagatcctc acccagatcc agcagatcgt ggatcagaag
    34681 tacggcggca gcgtgacgat cggtatccgg tcccaggacg tgcaagacat cgtgcggctg
    34741 tatgccctct cgaccgggca agcggcgaac ctgccgcggc ccatgtatgc cgccaccatc
    34801 gcgcagtcgc aggcgggagg cctcgccacc cagccggtct atcagggcgg cgtgctggtg
    34861 cagaatccct acaccggccc gaccacctac caataccaga ccgccgtcac cgccgcgcag
    34921 gggctgatgg cgggcaccag ccagggcgtg ccgggagccg gcgggcaggt gagcagtatt
    34981 ttcatgcagc tgaatccgca gcaggccaac gatctattca cgggccgggt ggtacaggcg
    35041 gtgcagcaga accccgcggc ggttgcttcc gccaacgctt ccgccacccg ggcgggagac
    35101 agccgggtgt cgcaagcctc gaacatgttg gagccgttga cgagtttaag ttgagaagga
    35161 gaagcaccta agtgaaacca acaatcggca gaatcgtgct gtataaccat ccgggtagcg
    35221 cggacggcag gtacccgcca acgcaatccc cggccatcat tcaaaacgtt gctgaagacg
    35281 gaacggtacg tttgttcgtc ttcggtccca aaggccagca catggatgac ggactgacgc
    35341 aaggcgacgg gccttgtcag tggaattggc caccgagagt gtagggtgcc gcagaatctc
    35401 aattccgcca acccgcaggg tgtgatgccg aagcagctgt gccggtcctt tcaggaggag
    35461 ttgcgcctgg aagcgctgct gaaccaatac gcggacggca gtagcgaccg caatgcgctg
    35521 gcacagaacg tgcggcactt cttccgcttg gtgcccgggg tgaacggcgc cgactgggcc
    35581 gccctgtggc agttctatat ccagcaccag ggtccggctt tctattttta caacctgcgc
    35641 gaaaccgtgc cgccgtttag ctacgacccc agcgggcaaa acaccatcgg ccgctacacg
    35701 gtggtcttcg acggccaatg gtcggacacc tacaacgtcg cgcgcacgga cgtcgccctg
    35761 cagctgcgcg aggtggcctg atggtccggg tcgaagtcag agacagcctg gggccagtgc
    35821 ccataccaag ccctccggta atttccgcct tccctcttcg gcccgacttc ggcggcggca
    35881 tcgactacaa tccggcggtc gtggtccaca ccttcgatca gccaggcctg aagacggagc
    35941 agcggttcct gctgacgccg atgggcgcgc ggcgcttcaa gttcgccaaa caccatctgt
    36001 cgtgccggga gtacgacgac ctcaaagccc acttcgagca ggcgcagggc agctatgcgc
    36061 agttccccta cacggtgtgg gagccggggc cggggcacgg cgtgccgcgt ccaacgcagc
    36121 cggtgtatgc caccgagaca gtcacagccc gctacgagaa tccgaatctg accttcgact
    36181 acatggtggc gctgttgatg aacggccccg gcctgagctt tctcgaagtc ccgcagacca
    36241 ccccgcaata ttattcggcg cagcggttga cccgattccc cgacgcgacc ttgaccgccg
    36301 ccttgcaagt ccagtttcag cagatcattc cgctgatcac ggtggtgccg cgttcgaatc
    36361 caaccgcggc ggtttatctt tccaaccagc acttcgcgct cgacagccag tcctacctgc
    36421 ccaggctgct cgactggtcc gggatctcgc agagtatcgg cgaaaattcc gattccgcca
    36481 gcttcacctt cggcaacgca gacggggtgt ggcagaccta cgtcaatcag gtcaatctgt
    36541 acgcggcttc ggttcaggtc agcctctacc atgtggggag ccgctacatc ctggacctct
    36601 ggaagggtgc gctgttgaat tggggcttcg acaccagcgg gcgcttccag gtgaacgccg
    36661 ccgacggcgt gttcaatctg agtctggcct acccgtcgcg caagattctg cgcacctgct
    36721 ggaaggtgta caaaggccgc tggtgcccat cgaccgcgga tccggggact ttccccgact
    36781 gtccaaagga ctacgacagc tgcgtggcgc gcggcgtgcc gcattccttc ggcgggctgg
    36841 tggtgccgcc gcagagcgtg catatcaagg acaactcgac cggcgtgttc ggctttggcc
    36901 ggtccagcat gacctcggtg acggtgacgc aggataccgt ctaccagcgt cccttgcagg
    36961 agatattcac cgacacgccg atgaacgtaa ccgtggacgt ggcggccggc cgcgacgaga
    37021 gtgacttcta tgcggcgctg ggcattgtgg gcgaaggccc gatctcggcg tacaacggca
    37081 acctgatctt gcagacgctg gacggccagc cgccgcagga ccccttgcgc ttcagcggtt
    37141 ggcgcgcctt caccggcacc gatccttcgg ggccatacga cttcgtcggc atcagccagg
    37201 ccaatccgct caccggcaag tgggccaacc cggacggttc tccgtatgtc ccggttggct
    37261 ccacgtatgc gggcgggatc gccctggcgg agatccgccg caccgaccag aagggcttgc
    37321 agctcgcgcc cgtggccgac cgcgccatga cggtgaatgt gacaggcgga atcggcggct
    37381 gggtgtggaa tgggccgggg cagcgcacct ggttgaactc gctgcacaac gcggtgtggg
    37441 tggcggtgaa cgtgtatctg cgcggcatcg ggctgcgcgt cgatccgagc aatgccgccc
    37501 aagtgccccc ctcggtcatg gagcagtatg tggacgtgga tcaggccggg atcgcggcgg
    37561 cgatctgcga caccatggtg cccaagatca tcggcgcggg gaacgaattg caattccctt
    37621 tccgtggcgt gctcaaagag cagaagccgc tgcgcgactg gctgcgcgaa attctgaact
    37681 gctgcctggg ctacttcacc ttcgtcaacg gcaagctctg gatcggcatc cgctccaact
    37741 caagtgtgct cgcgggcaat gccttcacgc gagcgagcgt gctttttaag agcctgaccg
    37801 cttcgccctt gcagccgcag ttcaactggc tggtgggaaa tttcggggac gaggagttcg
    37861 gctggcagct gaataatgtc accatctacg acatcgacca cgccagcttc ctgggcacgc
    37921 ccgactcgcc ccagtacctc accaacaccg tgagcttcgc gggcgtcagc aacaagagcc
    37981 agtgcgcccg cgtcatcatc accaagctgc gcgaggagat tggcggtctg aagagtggca
    38041 gcggcccgca cgggaccggc tccggcatcg acgagcagat gaatgcgcgc aacttccagt
    38101 tccgcaccac ggtgctggcg cttgggacgc tggtgggcga tatcgtctcc atagcccacg
    38161 acaccatgcc caatagcggc tatgccgagg gccgggtggc taagtggacc ctaaacccgg
    38221 atttgtccat cgatatgcag gcaacctcga ccaccgagga tatgtacgat ctggtggtcg
    38281 gtcccaaacc ggaggacgta tccgcgccca tggtgccgcc cgaggtgctg caagcggcca
    38341 ccggcctggc gtggatgccc aacgaggtgg cgccctcggc cggcgatccg gtctatccgc
    38401 cgtgggagcg caccttcgat ctctggcagg aatacgagat ttcgcgcgac gcggtgtggg
    38461 agccgaccat ctgggtgcaa ggcgagatgg cgatcaatca gttcgccatt ctgaagcagc
    38521 cgcgcatcct ggaaattgag gtccaaagcg gcggcaacct gaccggcccg ctgacggttt
    38581 acgcggcggt cacccagaag aacgcgggcg gggagccggc tgtgccgtcg aatctgacgg
    38641 ccatctacat ccccaccgga atgagcaatc agaaggtgac gcttacggtg gtgcccgccg
    38701 tcgacgccac cctgacggac tacgacgtct gggccggcct tgaccggcgg cggatggcct
    38761 ggcaattcgg cggcagcggc caaccgcccg gaacgatcga catccccggt cccatccacg
    38821 acatgaccag aggcctgccc gagggcgccg cggtgggtgt gcggatcgcc gccaagcacg
    38881 tctggcacgc cggggtggcg ggcctgctgg tcaacggggt cacctcgccc aacaagatcc
    38941 tgtgcaacga cttcctccac tcgaccgatg actgggtcgg ccagatggtg ttcgtgtgct
    39001 ccaacaagga cgggcaggtg ccgctctgga actttgagat cacggcattc gacccggcga
    39061 ccggcgtact tacagtgagc cccgattgcg cgaccgccga tcccacccag tccgtgcagc
    39121 agggcgatgt gctgatcgtc tacgcgcggc cgacctccgc cgacgccaac agcatcagca
    39181 actccctgtg ggataacagc gtcaaccgcc agcagtttcc ggggaccgcc ggcatgactc
    39241 ccggcgaaga gaagggcagg atcgtccgca tcctgcgcaa tacgggcgcg gggcaatggc
    39301 gcttcgtcac cgacaatacc gcgctctccc attcgatctc tcctccgtgg gatgtgatcc
    39361 ccgacagcac ctcgctctat atcgtcgagg ccgccgactg gcccagcatc agcccgacca
    39421 accagctgat cgtttcgcac tccggcatct ctaccgcgct gcacctgcag gtgcctaatc
    39481 tgaccgacga ggtggcgctg gtgggaggct tcctggtgga cacggagggc cggcaaaccg
    39541 acgatagcgt ggccgccttc cgcatgatct ttgtgttcgg ccagccgccc acggtgcgtg
    39601 tggtggggcc tgaccgcggc cccttcgatg tcgccgtgac cgaccagacc atccgcgcgg
    39661 atacctcgca gaacgacgtc actgtcaacc tgccgcctct cgccgtgctc caagggcgca
    39721 gcctgctgtt cgtgacggac gggccgaata ccaccatgat caacacctcg cctccggata
    39781 agttcccgga cggctccgtg cggatcgcgc ttggggctgg agccaccgtt cgtatcaccg
    39841 caggagggat ttacaccaca tgaacggtcc ccttcggcca ctaaggaacg cccgcgtctc
    39901 gagaccgcac gtgcccaagg catcgcctac taccggctcc tggattgtgg agaccgacgg
    39961 gaccggcagc agcgggggct acatcccacc ggatgctcca ccggtctcgg tcggcatacc
    40021 agaagtgaac ccgctttacg atggccgctt cgaagtgcgc gttccctgga cgccggaccc
    40081 gaccgccggc agcggcaact tccagggcgt ctcggtctac gtcgaagacc ccgacatgag
    40141 cgagaccacg accgcgccga tggatggcac ggtgccgatg gacggcacga ttcaaatggc
    40201 cggcaagtgg atttggcagc acgtcaccga aaacactgaa tctccggccg acttgttcgt
    40261 aaacggcaag gtcagcgatc gctacatccg ggtgtatttg caggcttaca acttcgtcgt
    40321 caactccacg ttgatccggg ccaacaagcc caacgccact ccgaacgtgc gggttccgat
    40381 cccggccgcg gcgggccact atgtgagcgg ccaggaatac gcctggctgg tctcgaatcc
    40441 catcgtcacc gtaatcgaca atttcgataa cccggaagac cctacttaca gcctcgtgtt
    40501 ctcgttcatt gaaccggacg acaccattcc cctgccgccc gggttgaagc cctacgccgg
    40561 ggtgcagacg gtctacgagt acccatccca gaacaaccgg cgcgcgcagg cccgtttcct
    40621 gacggcgcac aagcccgaga cctggatctc ggacgaatac tacgcaacca ccatcacctt
    40681 cctggtgtgg ttctgttcgg tcgattccga gggccacgtc aacaccatcg tgaagggcgt
    40741 caccccgatg gtgcaggtgt cggtcactta cccgcccgca ggccagacca tttcgcccga
    40801 ggtcaccggc cttgccattg ggccgacgac ttacctgccg acgcccgacg gctccatcct
    40861 ggcgcaagcg cagctcacct ggaccaaccc ggcgacgttg aaccgattca gcgggattca
    40921 tatctaccgc gtcgggatca cgccgccgcg cagcttcgga gtgggcggaa acaacgccac
    40981 cagctttacc ctcgaagcgc tcgactggcc acccgtcacc gcggagagct ggactatcgc
    41041 cgccatctcg ctcgacgaca agggacgcct cgcggacgac cctgcgtcga tctctgcggc
    41101 tacgccgacg gcaatctggc acatcgggcc gccttccatt tcgaacggcc agtacgcgcc
    41161 cgtggtggtc gccaccgcgg ccacagtagt ggcggaacag caactcaatt ccgatggcgt
    41221 agtgatggtc cgcttcgtca tctcggggtg ggtgaactcg actgataaca aattcggcgg
    41281 cgtctccatc gcgcggctgc tggccaacga ttccttgacc accggcaaaa ccacctggtg
    41341 ggacgctccc aaaaatcaga cctcctttac caccgactgg gagcccgcgc ccgctgcgac
    41401 ctcctgggat ttctggtttg tgtcgcgcga tcagcagggt aaccgcaacc agatcctgcc
    41461 ggggatcacg cccaaggtga cggtcaactt caccccgcaa gcgggcgacg tgctggcatc
    41521 gcgcctgccc aaagactggt tcgatacctc ggaatttcaa tggcccgatg gtttcgattt
    41581 ttcggtcaag ctgattcacg ccgagaagat ttttgtggac aaggtcctgc gcgtcggcgg
    41641 cggcaccggc acgctggcgt cctccttcgc cggaaatcag aatgggcaaa tcggcgtctt
    41701 taattttcag aacacgctgg tcggatggat cggccagcag gacggcacgt cgactggcga
    41761 caatcccacg ccgcacagca tttacggcgc atggttcgct caggtctaca tcggcggcgg
    41821 caaccccacc accgcgccca tctatatcaa cgaccagggt gtggcgatct tcggcggctt
    41881 cgatacgcag attgccaacg gcgcaacacg gtacccgtac atctcgatcc gggacgcctc
    41941 caacgttgag aagggccgta tcggagcgcg gattggcgac ggtctggtga cgccggccga
    42001 tccggcctat attgcgggcg cgtggttcac cgagttcgcc gtcggcggtc agtcgctcgc
    42061 cgactggcgc atcctcgcca agcgctcagc caccacggat ccgaccgagg gcagtctggt
    42121 ccagctgcgg aacatcaata tgttccagat caattacgtg cagaactacc actacccatc
    42181 gggcggcagc aatcatccga acgcggccta cactttccaa ctcgggtacg acgcctatgt
    42241 gacggacgga accactagcg cctattccaa attccccggc ctcacgctgg tgcgcgatgg
    42301 aacctcgcac ggcatcagcc tgatcaatcg cggcctgatc tgctacatgc caaacggagc
    42361 gcgggcggcg gcgctggtga cctacaacgg cgatcccaac ggcggcgacg gcgggcagtt
    42421 ctgggcggaa atgactctgt actcgcaggc gaatagcgcg atcaacgtcg agctttcctc
    42481 cgggacctcg ggaagcggcg cttcgttttt caagctctgg gatgcgggga gcaacctaaa
    42541 tttctcggta gatggggcgg ggacggtgtt ctgccggagc ggactgacct gcgccagctt
    42601 gacggtcaac ggcgctctga cggttaacag cctgaccgta aactcggcca tgatcgcaca
    42661 gtcttacaag gtgggctcca ccgaggtcat cactgcgggc gggcagttcg atggaccggg
    42721 cggcgtcaac acgaccggcg acatccgcac gggcgggcaa gtggtgggcg cttccttcta
    42781 cctgccggga ggaagccagc cgctgattac caacgcgggt ggggggcagt ggaatggagc
    42841 aggcggcgtt tacaccaacg gcgaaatcga gagcacatcg gtagtgaagt gcaggacagg
    42901 cttttatgtg aacggagtag gaggggcgaa cggatctttc gtggctggcg cgaaaacggt
    42961 caccgtgtcg ggtggcatca tcacatcgat tgtttaagga agaggagaaa catggaacga
    43021 agttttcggt tactggatta cgaagcggag caacaaacgc gcatggagca ggagttgcag
    43081 aacacgcttg ccaactatgg cgctctcact ctgcaactgg aagcggtcaa ggcccgcatc
    43141 ccgcaggtcg aggaacggca gcggaccttt gtggggcagg tggcggcgcg ggccaacgcg
    43201 gggcagtttc agagcgccag gattgcgaac ggccatctga tctgcacggt gcccgatgag
    43261 ccgatggggc tgccgaaccc ggtgccggcg gggctaccag tgctggatag cgcgggtggc
    43321 ccggtggcag ttcccgaaca tcgtgccgcc gaaggccgca agccgcgcgt caatgggccg
    43381 gatcagataa gcgcaaagga gtaagtcgat ggcttccgat tgcagcgatg ttatccggcg
    43441 gacgatagaa gtcatcggca accggcgcga agcaatgcaa tggctgggca cgccggttcg
    43501 cgccctcggg tacgccacgc cgattgcgcg actggcaagc gaggccggaa cagaagaagt
    43561 cctgaatctg ctgaccgcac tggagcatgg cgttctggag cccgtgggca ttacgggcac
    43621 ggtgggcgtt atcacactcg tgggcggaat cgtcgttgaa tatcggctga aggagtagat
    43681 caccatgccg tggctgtcgc tcaataccta cgttccgact gacaaggttc ggaacgatat
    43741 gttcaatagt ttggccaacg atgaccgcaa ctggggcggc aacgtgaacg gcggcggcaa
    43801 ccggctgagc aacgtcatcc tcgacggcac aggcggcttt cagttctacg cttcgccgct
    43861 ccaggtcacc ccgggcgcgg atggccaggg cgtcgtgcag atggatcaga ccgtcggggc
    43921 caaccagatc gcgcgatgga cagcgggcaa aaccgccacc gccgagagcg gggcaaacgc
    43981 cggttccgac tatgccatcg tccgctacga cgatgccggc gctctgctgg gcactccctt
    44041 ccagatcgcc cgctcgaccg ggctgattac gatgggcgct cagaagtgga ccggcgcgat
    44101 tgacggcggc gggcagacgc tctcgaacat caccatcgcc ggagtggcct ccgatccaac
    44161 caccaccaag ggcgacctga tcgcgcgggg cgcggcggtg ctagggcggt tggcggtcgg
    44221 caccaatggg caggcgctcg tagcggactc tgcacagact ctcggcatga agtgggccac
    44281 caccgatccc actacgacgc tcggagatct gatcgtgcgc ggggcggcgg cggtcgggcg
    44341 gctcggcgtc ggcaccaacg ggcaggtgct tcaggccgat tccacgcaga cgttcggcgt
    44401 gaagtgggca gcggcggcga ccacggcatc ctactcgaag gacggcacgc tggttgggac
    44461 acgtcccggc ttcaacgtca tcactggcgc gaacgtggtg ctgacgatgg cggatgactc
    44521 ggccaacaac cgcgtcaacc tcaccatcgc ggcggcgggc ggcggctcgg gcgggatggt
    44581 cgacccgacc acaacgctcg gggacctgat tgtgcgcggc tccgctgcgc cggtgcggtt
    44641 cgcggtcggc agcaacgggc aggtgctcac ggcggattcg tcggcggcgc tcggcgtcaa
    44701 gtgggcggtg ggcggcggcg ggcaaacgcc gtggacaagc gatatcaatg cggcgaattt
    44761 tgggttgtgg aacttacgct acctcgctat tggtgcagcc tctttgcagc catcgcccac
    44821 tgtcggcttt ggggttgcag gaagcagtgg tcttacgtca gtggttatta cagaaacgct
    44881 gagcaccggg caggcaggct tcactctcaa cgccaacgtt gcccaagcca ctttcatgtt
    44941 gagcgggagc gcggcgagtg tcccgaatca tctggcgctg agtaataaca ccggaggtat
    45001 ttcattctgg acagggaccg agcgcctgcg catcacggcg acggggaatg tcggcatcaa
    45061 tatcacaact ccaggttccc tgctccatct cgcgggaaat ggcgctgtag atacgcctca
    45121 aggcgtgatc acaatgtccc ggttttggcc tgatgcctca aacgtgcggg caagcgccct
    45181 tttccactat tacaattccg cagcggctac ggatttgctc gccttcggag tttccgggga
    45241 cggcggtggc tcaactaccg cacccggaca aatcagcaac gccaagatgg ttatcacggc
    45301 gggcggttat gctggcatcg gcacgacgac tccgacgctt gaggccgcaa tggccagact
    45361 tgtggtaact ggcatagcag cgcaggtggc ctcgaatctt gccacaagca acacaaaagc
    45421 gctggcttct ttccgggcta atagcagttc cggttataca ctcgccatcg gggccgagag
    45481 cggcaattct ttcccgtact tacaggccgt caattggaat ggcggtagct cggcggcgtc
    45541 tctgctgcta aatccattcg gcggcaacat tggcatgctc aaaatcaacc cagcttacgc
    45601 gctcgacgtc gcgggcgacg tgaattgcac gggcgcgttc cgcgtcaacg gcgtggccat
    45661 tggcggcgct caaacaccgt ggacaggaga cgtggatgcg gcggggcacg cattgaacaa
    45721 tgtcagcgcg gtctacagca acatcgcatc cgtaggcggc acgggtaaat atttaatatt
    45781 tggctgtcca ctggccacgg gtgatcctgg cacaatggca gcggacacca ttgccttttg
    45841 gcgtgactca tccaatcaac tacacattgt gtgttcttac agcgatctaa cacgccaccg
    45901 cgcaaccata ggtctggcct agagcgaata ggagagaaac taacatggca cttacttacg
    45961 atcaatccaa caccctgatg caggacgcgg ctttccgcgg ccgcgtcaag gtggcttgct
    46021 taaaatacgc ggactcgatt ctcaacgaag cgccgaccgt gccggcgcac aacacgcggt
    46081 atcgctgggc gcagcagtgc gaccaacagc cggaccagac cgcgctgacc atcacgccgc
    46141 cgaccgtgat ggacccggcg gtgcagcaag acggctccaa catcaccgat acggccttgc
    46201 aggggtcggt cgagaccgtc atcaacaaac tgctctaagg cttcgggcgg gagcgcttga
    46261 ggcgtttgag ttcccgttcg aacacgatgg cgaactccac gaagctgata tccagcttcc
    46321 agagcagctt caaaaccgtg tccagcgtgg gcgagtgcag gccgcgttcg agcccgctca
    46381 tgtagccgcg gtccacgtcg gagtcgaagg ccaggcgctc ctgcgcgacc tgagcggtgg
    46441 cgcgtaacgc gcggatggcg cggccgaagg ccaaccgaac cgatggcaaa tcgcccgttg
    46501 ctggcttccg gggcatatag gcatagtgca aggaactgca caaaaactac cacgcgatag
    46561 atgccacggg atagatcaga ctagccgcaa ccatattgtg taacaactgt actatcccta
    46621 tgggccgtgc gcccttactc acagtaccga tgtgagtcct aggaaagttt ggttgcaatt
    46681 tcgcggtaga gggtcatact tcttaacaac atatctttca gcaaacgttt gatggatggg
    46741 cgagcttctt cggcacctgt taacgggcgg gtccgcgcgg ccccacagct ttggaatact
    46801 tcgatggttc cgagtcgcag tccgcccgcc tacccactag agccaccgta ctgcctcgat
    46861 attgcaaccg tactttcgca gcaccgtggc gacaccacag cagcactgtg ggagcaccgc
    46921 agatgtgccg ccatcgtgcg cgcgagagca ccgcggcggt gctaaccgct gtgttgccta
    46981 taatttacgc ttgacacagc gcaagtgctg cgttagtatt ctgcgtgttg atcgcacccc
    47041 gaccgggcga cagcacgcgg agccaaaaaa caatgcccaa acacttacgg gagagaactg
    47101 tgcccaaaat cacgaagtcc gcagagttac ggcccgctgg gttttccaga accaatatgc
    47161 agctgccggt tggtctgatc gatgcgatga gaaaactccg gcatatccgg caccagcagg
    47221 agggcgtgga ccccaagctc tgccggatct acaaggaagc cgtggagcag tacgtcaacg
    47281 cgaaaccgca gcagcaactg ctgcaggagt ggtacggaga tggacctgga aagccggcca
    47341 gagcctctgg cgaccgcgct gccgcgcggg tgtgatgtct gtcaccagca ggccgccgtg
    47401 cgctggctgg attacgacct ctgggcgtgc gccgactgcg cacgcctcta tctgacacag
    47461 ctgcaactga aaggctgaac cgtcatgcaa acagcaacca ttgaaatcga ccgttcgcaa
    47521 gcgatgaccc tgtaccgcca gtacaaggag caccgcgact gcttcaccaa gatggacgcg
    47581 gagatcgagc gtgcctaccg gcagatcgcg cgcggccaca aggtgattca ggcgctcgaa
    47641 agcatccgtc acgccggcct cgacgccctg agccgcccgc gtctggggat cgtccgggcg
    47701 gatgccgagt ggtgttatct gacacgcggc aacgggcagg tgttgttctc gatggaccga
    47761 tgggtgcgtg ctcgcgcgcc acgccgctcg gtgatcgtgc gctggcccgg catgatctgg
    47821 aacatcgaca acggaggcgc caaggcgcgt gtgccgctga tcccggtgca tctccggccg
    47881 cgcgtgagcc tggaccggta ccaccttctg tttgaagcgg actgggtggg ggtgccgccc
    47941 gacccgatgt tgttgcggcg ctgcggcaac gacctttggg tggtggtggc agcgtgggat
    48001 ttgacgccgg tagagcgcgc cgtcatgggc gcggagtaat gccatgatcg accaccaact
    48061 caggaacgcg tgcatcacct cgacggatgt ggggccgatc ttcggggtgg acgcctaccg
    48121 gggagccttc gacgtgtggg ccgtgaagca cggacacgcg ccgcggtgga acccaactcc
    48181 gcggatgctc ctgggcaagg acctggagca gggcatcttg caggcctaca gccggatcac
    48241 cggcaggccg gtggagtggt tcgacaagac catgcggcat cccgagcggc cgtggatggc
    48301 ggccagcccc gacgcgctgg taaccagcga tccgctggag cgcggggtgg acgccaagct
    48361 ggtcttctgg gaccagcggc gtaagtgggg cgcgaactcc aacgacatcc ccgaaggcat
    48421 ccagatgcag atgtggtggt tcatggcggt cctcgaatgc gacgtctggg acgtggcggc
    48481 ctgggtgggc gaggatgagc cgcgcatcta cgagatcgag cgcgaccgcg aagccgagcg
    48541 ggtggtgatt gccaagtgcg aggaatggca ccgccgctat gtggtgggcg acgaggtccc
    48601 gccgatctct ggcagtgaaa cgtcgtcggc ctggctccag caggcgtttc cgcacgacca
    48661 tcggccggac atgcgcgacg cgaccgatgc tgagatcgcc tacctggagg agtacgcgct
    48721 ggctcggatc gaccagcaag aagccaccga acgccgcggg gagatggaga atcagatcaa
    48781 actggcgatt ggcgaccgcg agggcctgca atggccgggc ggtaagttca catggcggcg
    48841 caccaaggac caggaaaaga tcaactggaa agcgctgggc acatacctgc taaatacccg
    48901 cattcgggaa gagcaggaac gcgaggcgat gcgcgtcgag tacacgaata ccaaagccgg
    48961 ggtgcggcgg attcatttcg tctgcgacac gcatgatatc tccgacgctg tgggagtgac
    49021 ggggtgaaga tcgagcaact cagtccggag gcgcaggacg gcctgcgcaa gtgggccgcg
    49081 ttccagcacg atgatttgct cttcgacgcc atcagcagtg tgccgcagga gcgggcctgg
    49141 atctggtttc agctatgcgc ggcggaagac aggcccgagt ggtttcccaa ggggaagtgg
    49201 gcaaccatcc agcacatcgc cacgattctg gacgaggaag cggatgcgac gagaatacga
    49261 ccctcaatgt gaagccctcg cccgctattt cctgggccaa cccgcgcgcg atgaagccct
    49321 cgtggtggag ctggccgggg tgattcagga ggccatcgag gactgggaac agaaccgaca
    49381 gaaggagaaa acaagcgatg caaccgacac caagcaacac aaccgttgac gtagcggtga
    49441 ccccgccaac cgcgccgccg ccggcgccgg gagctatacc caccgaggcg cgctctcggg
    49501 taacagtcga gcagttggcc acgttgcccg acagcgaacg gcgcaccaat tggatcaact
    49561 cgctggtcga cgccgaggtc gcgcggcaga actacgcgca agaccgtgcg atggcgaggg
    49621 agttcgccct gtgcgggcag ttcgacgatc tcaaaggagt gagcaccgag caggccgtcg
    49681 ccacggcgat ggtgaaaatc aacctgggcc gctcgtgggg attcaactgc gctgactcca
    49741 tgcggaatat ttatttcgtg aacggcaagc ccgcgctgga gcaagatatc gtagcgagta
    49801 agttgcaaca ggcgggaatc gcttgggacc ccgagttcgc ctatgaggaa gtgcccgcaa
    49861 ctaagaccgg gcgcgcgtgg cgcaaatgcg taggatgcac cttgtggctg aaggagtgga
    49921 acaagatcga gcagcgctac cagccaatca aagatcgcaa gggcgaacag gtggcggtgt
    49981 cgttcaccct ggccgacgcg gagaacgcga agtactggga aaagggtcag gaaaagccgc
    50041 tctccgagaa gttcaactac cgcagttatc caggggacat gttctattgg aggtgcatca
    50101 gccgggtgcg caaatactac gcgccgcacg tcctgcgcgg cggcatgctg cgtgaagagg
    50161 cgctggagat gatgcccgcc gatctgccgc ccgaccagct gccgcgcgaa ttgcaaccgc
    50221 cggaagtgct accggccgag cggaaaacct tggcggagag agtgaaggcc aacgaagtcg
    50281 ggccggacgt aggcacgcag aacgccatgt ttcccgagta gtgccatgac cccggagcca
    50341 gagcacgact gtctgcaata cctgcaatat cgggagcata gctgggtcga gacgcacggc
    50401 ctcgatgcgc caccctacga ggcctgctgg caggaatggt gggagtgcgc tgtctgtggg
    50461 tcgcggttta cctcaaagga gttgagagag atttatgagt cgcaaacgac agtggggcga
    50521 ggattcgacg acgaagattg atctttccgg gggcgcgtcc atcacggtgt cgttcctcgg
    50581 aaacctcttc actctggagc cggctgagcg gcagctgatg agcgaccttt ccgccgtcct
    50641 ccagagctat caggacagca aagcatcggg ccaggcgaag gcggcggcat taaaatgacc
    50701 acggcatcca caactcgtaa atgcgcctgc ggccgcacga tcagtggcaa caaactgacg
    50761 tgtgccgctt gctcgaagcc ggtcgcgact ggcgatggcg tgctggaaat cttgaacgtg
    50821 ggagcggggg acgtgaagat cacattcaac accggcgacg tatccgatac gattcgtgcg
    50881 cgccgcattg tcaccgacat gctgcgccgt ggcttcgcac tgctagtcga gatcgagcgt
    50941 gacggcgagc ggaaatacga gcgcgtgcag gccttcgatg aaaagaccgg cgagtatctg
    51001 attgccgact tcgatccgct ggacgcgcgg caagccgatc tagaagacgc tgctacagcc
    51061 cgccggctgg agacggccca acggcatggg gaagcaccaa ccggacttca gcccccgggc
    51121 agtagcagcg accttgggat cacggtgaag gatattcctt tgtgcgcctg cgggcgaccg
    51181 ctgcaccatc gcggctcgtg taagggccag aagcgcggct atcgccgggt gccgatggaa
    51241 ggcgcgaagg ccacggcagt ggggaggtca agcggtggct aggcctacac cgtcttgggc
    51301 ggacgttcgc aaggggatgg cgcggatggc cgagaagcag cacgagtggg ccggcttccc
    51361 gatgccggtc gagggcatgt cgatgcagat ccatccgagc tatccgttcg cggagaacct
    51421 cagcaagacc tttctgccga aaccgtccct gcgcatgtgc cgcgattcgg acgtgtgcga
    51481 agagacgcgc ctacggaacc gctggaactc ctaccgcgac ggtaaggaga tcctgatcgt
    51541 ccaggagcgc gggaagtttt tctttctcta ctcgccggtg cagaacagcg ggccgatgct
    51601 gctgcaaacg gtgagcgccg cgcgggcgtg ggatttcgag gccgagcttc gcgcgatgga
    51661 gacactcaag cggcacgtta ccgagtgggc gtttcagtgc tacgtgatga ccggctcgtt
    51721 ccttgaaacc tcgaagcgct cgggggtggt gtacctgttc cggcggttgc ggcctacgat
    51781 tgccttgact ggcaagcccg acaagaaccg ccgcgatgtc gggatgcgga tcctctgcac
    51841 gctctgcgct caccccatcg gttactaccg cgattcgtgg gccggcgccc tggtgccgac
    51901 cgacgatgtg ctgagtcacc tcttgctcat gagggccgat gaaaagtttt tttggcgcaa
    51961 tagcaatcaa catccggcgt gggcgccgga gtcggggtta taaaaccgcg atggcgatga
    52021 caccagcgcg gcgaggtcag gcggaggcag ggctcggcag ggttttttga aaagaaggtt
    52081 tttaatttgc cggggcaggg tgtggcacgg ctgggcacgg cacggccggg cagggcaggg
    52141 cagggcaggg cagggtacgg cgaggcgagg gtgccaatgg cacaaatcaa ctaagaaggg
    52201 cagaaacaac aactatgaga accattgaag tggaacttac cagcgaaatg ccgctcttaa
    52261 tgcacgcgga taacatcgac tgggcagaca agatggaaga ctggaagaac gatcccaaga
    52321 acaagagtaa gtcaaagcca ggagacgata gaactcccgc ctggaggtgg ctcgggtgcc
    52381 taaactacga tgatccgaag accggcgtcg tgaccgttcc atccgaatac atcatgcggt
    52441 gcctcatggg aggcgcagcg gaagtaccga ccggcaaggg ccggaagacc tttaaggaac
    52501 tgagccagag cggcattcta tgcgaaaaat ttcactggcc tttgctaatc aatggggaaa
    52561 agaagccggt gcgaatggag gatatcaatc aattattgaa ggtcccccgt ttcccggacc
    52621 aggtcgaggg cgctaaggcg ctgggcttct ctctctttgt caaacgcgca agagtcggca
    52681 ataacaaaca tatccgagtc cggccgcgtt ttgacgattg gagtacgaga ggtgaactga
    52741 tgatcatcga cgatcagatc accaatgatg tggtcgctag tattctcgac atctcgggaa
    52801 ggttgaaggg tcttggagat tggaggccga gcgctaagac tccgggaccg ttcgggacgt
    52861 tcaaggcgaa gattgtgagt taaggcatgg ctcggcgcgg tagggtcggg ctgggcctgg
    52921 cctggcaagg ccgggcgcgg tgcggcatgg ttgggcaggg cgagggtgcc aatggcacaa
    52981 atgaaaggag gaagttatga gtcatggatt tcgcggcggc attccctacg cggttgacgt
    53041 gaaccgcctg aaggatgctt tcccggtgcc gatgctcgcc gaagggcgag tcattgaaca
    53101 tgccgactta gaagaaatcg tgaaggcgaa gaggggcggg cagcgctact acggagtcat
    53161 caactcctgg atcaatagca tgaaaaacac caacggaatt ttcatgacct gggagacgtc
    53221 ggtgggcgta aaggttctgg gcccggcgga aattctagct cacgctgaga tcaggacccg
    53281 gcagaagatc aagcagaccg gcaatgcggt gaagaccttc gtatgggtcg accgaacgag
    53341 attggacagc acggggcagc agcgtttgga tcaccaggag cgcgtcgcca acgcgatcag
    53401 ggatgcgctg aacactgcca gaaaagattt ggcgatagac ctggcgccgg taaagtctct
    53461 gccaaaaccg aaaatgattc gggaggcctg aagaagtttg gtccggtcgg gctcggcaag
    53521 gcccggtggg gctaggcgtg gcggggtgcg gctaggcaag gttgggtatg gtacggcgag
    53581 gcaagggtgc caatggcaca cgggattgtg atttcggtac ggcgaggcga ggcctggcag
    53641 ggtgtgggca tggtgtggcg aggcgaggcg aggtatggca gggcgagggt gccaatggca
    53701 catgagaatt catttcgtaa ggtttgcggc gagggcaggg caaggtcggg tcaggctcgg
    53761 ctcggtcagg caggccaagg ctgggtcagg tcaggtgggg tggggcatgg tcgggctcgg
    53821 tcaggcaagg ccgggcaggg cagggtacgg cgaggcgagg gtgccaatgg cacgaacggg
    53881 atgtttgcaa gttccggcaa tgcgcggctg ggctgggcgc ggcgaggcac ggcaggccag
    53941 gaggaaccag cgggtttcca atgggaaatg tcaaaatgag ccatgcaatc acaaaagcct
    54001 agtacagaac aacaggagcg cccgcagctt ccgtggggca tgggcagcgc caggatcaag
    54061 ggccgcgtct ggtggattac gtaccgagat ccgaaaggca agatacacct cgaaaattcc
    54121 ggcaccgggg atcccgccga ggcccaacgg atcatggcac agaaggcttt gccacgagcg
    54181 tgggcaatgg tcgcggcctt ggagaagtta gccaatgcag aaccccaagg tcccaacagt
    54241 aagggtggta agcggccacg atctggatct gtcgcgccgg tcggcactgc ggctgctgcg
    54301 gctgctcgaa aggctggcgg caaagcgact cgcaagggag gcaaagcgtg acgcgaaaga
    54361 ctgatctccc ttggttcgtc tatatccggg taagctccaa agaccaaggc gacaagtacg
    54421 ggccgtcgag acagatcgag gccattcgga actggctgca ggtcaatgtg ccccatgtcc
    54481 gcgtgccggg actggatacc tgcatcgctg ggccgcgcga tgtgcggcag tgcgagtatg
    54541 ttggattcga caagcagagc ggcaagaccg acgagcggcc cgactttcag cgcggctgcg
    54601 agttagcgaa ggcaggcagg atcggcgggt tcattgccct ccggttggat cgagtggcgc
    54661 gcaatgcggg agacgcctac ctgcttcgct ccaggatgaa gcgcatgggc gtgcggctgg
    54721 aattcgccac ccagacgttc gacgccacgc cgaccggcga cctgatgtat caggtgtacg
    54781 cgagtttcgc ggagttagaa gggaagctga ttttggagcg caccgcggat ggccgcatgg
    54841 gccgtctgtg cggcgacaag ttattccaca accccaacgc gatgccttac ggttacctct
    54901 acgtggatga agcggtcgcg aaaaagaatc cgggcgcggt catcggcagg gcgatcatca
    54961 acgagcaaga ggccaagatc gtgcgcctga tcttccgctg gtacgtgg
//
LOCUS       DASQOP010000281         2867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_543733, whole genome shotgun sequence.
ACCESSION   DASQOP010000281 DASQOP010000000
VERSION     DASQOP010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543733"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VE030_11385"
     CDS             <1..380
                     /locus_tag="VE030_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZF24053.1"
                     /translation="DASAVLHLASAGMKVSGRHKLLAPTLLRSQTLSALHEAVQRGEI
                     PADVARDRLTRIGRMPIRLLGDAVLRRRAWELADRLGWASTYNAEYVALTQLQADAFV
                     TLDAELARSVEGVIATASIDALR"
     gene            660..977
                     /locus_tag="VE030_11390"
     CDS             660..977
                     /locus_tag="VE030_11390"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HZF24054.1"
                     /translation="MSTQAHVRIERGTRCFLSGRCRLPNSYLYDKEIIPRVEKAILAD
                     GRFADTAVWAEGQRRWVSLKGCVRSKEQAKALEQLVRQLDDVEAVINQLVVSPHSQGA
                     FHE"
     gene            970..1452
                     /locus_tag="VE030_11395"
     CDS             970..1452
                     /locus_tag="VE030_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24055.1"
                     /translation="MNKTKFIATFLVAAVLSTLAGCASLPSPEVMKAEVASFNVPKLP
                     DPGKAICYVVRPSGLGGLVRFNVFVDDQQDSSEVGYTRSSEYIYFSLAPGERKIYSKA
                     ENWAEIQVSARAGDVIFVQQEPSMGIIMARNSMVKLEDYQGKYYVKTLQVGTIIKTDK
                     "
     gene            1457..2068
                     /locus_tag="VE030_11400"
     CDS             1457..2068
                     /locus_tag="VE030_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HZF24056.1"
                     /translation="MDLNTWLIYLLAAIGLSLSPGPNGLLALTHGALHGRRKAMYTIF
                     GGAFGFIAVVAFSMLGIGALLKTSLVWLTVMKWVGGAYLVWLGIQVWRSPPIGIEVRG
                     AAEPRAGWSLFQQGALSALTNPKALLFFTAFLPQFIDPARSLFVQFVIMAGTFAVLEI
                     ATEVFIANMAHRINPWLRRVGRRFNQACGGVFMAIGVALPLRS"
     gene            2072..>2867
                     /locus_tag="VE030_11405"
     CDS             2072..>2867
                     /locus_tag="VE030_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HZF24057.1"
                     /translation="MGLSPFALSKSHARRLWLRAQRLDEYAPFGDGPGATRAAVEHLG
                     YVQIDTINVIERCHHQILYTRIPGYRRDHLRQAQTVDKTVFEYWTHALSYVPTRDLRF
                     YLGAMKWYRQHRSPWFAKVKEEDLRRVLALIRKQGALTIRDIDDDVLVEKDHAWASRK
                     PSKRVLQLAFYQGTVTISERTGMLKTYELTSRHFGWQRPPKPASEREVLDYQLDRALR
                     AQGVVSVDSACYLDAPRKPAMRRLIEARLRRKELVSVELEGAGKLQH"
BASE COUNT          562 a          872 c          885 g          548 t
ORIGIN      
        1 tcgacgccag cgccgtgctc cacctggcga gcgcagggat gaaggtctcg ggacgccaca
       61 agctcctcgc acccacgctc ctacgttccc aaacgctgtc tgctctgcac gaggctgttc
      121 aacgcggaga gatcccggcc gacgtcgcgc gcgaccgcct aacacggatc ggacggatgc
      181 cgatcaggct ccttggcgat gccgtactcc ggcgtcgggc ttgggagctg gcggatcggt
      241 tgggatgggc ctcgacctac aacgccgagt acgttgcact cactcagctg caggcggacg
      301 ctttcgtaac tctggacgcg gaactggcgc gcagtgtcga aggcgtcatc gcgactgctt
      361 cgattgatgc gttgcgctag gactgcgatg catgccgcaa ttttccgatg ctgatatgga
      421 gcagaattga ggcggtaagg tgccgcatgc atcgcgcacg aaccttgaaa cctggcatcg
      481 cgcaacatgg gttggcgagg gcagcgccag tactgctcgg agccatggcg gcagcgctca
      541 ttggcggacc ggccttggcc gaaaagcgcg cgaattactt caacgatccc tttcttcagg
      601 ttaccagagg gatcgctgac tgtcctgtgc cggagggtcc aatgatcacg gaggctgaga
      661 tgagtaccca agcgcacgta aggattgaac gcggtacgcg ttgcttcctg tccgggcgct
      721 gccggcttcc gaactcgtat ctctacgaca aggagatcat tccacgcgtc gagaaggcta
      781 tcctcgccga cggtcgcttc gctgacaccg ccgtctgggc cgaaggccag cgccggtggg
      841 tatcgctcaa gggctgtgtc cggagcaagg agcaagccaa agcgctggaa cagctggtgc
      901 gccagctaga tgacgtcgag gcggtgatca atcaacttgt ggtcagtccc cattcccagg
      961 gagcattcca tgaataaaac gaagttcatc gcaacatttc tggttgcagc tgtactttcc
     1021 actctcgcag gatgcgcaag ccttccttcg ccggaagtca tgaaagcgga agtagcctca
     1081 tttaacgtgc cgaaactgcc cgaccccggc aaagcgatct gctatgtcgt gcgaccgtca
     1141 ggtcttggcg gcctggttcg tttcaacgta ttcgtcgacg atcagcagga ctcctcggaa
     1201 gtgggctata cccggtcgag cgaatatatc tatttcagcc tggcccccgg ggaacgcaag
     1261 atctactcca aggcggaaaa ctgggcggaa attcaggttt ccgcccgagc tggagacgta
     1321 attttcgttc agcaggaacc atcgatggga atcatcatgg cgcgcaatag catggtcaag
     1381 ctcgaagact atcaagggaa gtactacgtc aagaccttgc aagtcggaac gatcatcaaa
     1441 accgataagt gagcttatgg acctcaacac ctggctcatc tacctcctgg cggccatcgg
     1501 gctttctcta tcgcccggac ctaacgggct gcttgccctg acgcacgggg cattgcacgg
     1561 ccggcgcaag gcgatgtaca ccatcttcgg gggcgccttc ggcttcatcg ccgtcgttgc
     1621 cttctccatg cttggcatcg gcgcgttgct caagacttcc ctggtgtggc tgacggtcat
     1681 gaagtgggtg ggcggtgcct acctcgtctg gctcggcatt caagtctggc gctcgccgcc
     1741 gatcggcatc gaagtccgcg gtgcagcgga acccagagca ggctggtcgc tgttccagca
     1801 aggagcgctc tccgcgctga ccaatcccaa ggcgcttctc ttcttcaccg cctttcttcc
     1861 gcaattcatc gatcctgctc gaagtctctt cgtccagttc gtcatcatgg ctggaacatt
     1921 cgcggtactc gaaatcgcca ccgaggtgtt catcgccaat atggctcacc gcatcaaccc
     1981 atggcttcgg cgagtcgggc ggcgcttcaa ccaggcctgc ggcggggtat tcatggcgat
     2041 aggggttgca ctgccccttc gcagttgagc ggtgggacta agtccctttg cgctcagcaa
     2101 atcgcacgcc aggcggctat ggctgcgcgc ccagaggctc gacgaatacg cgccgtttgg
     2161 cgacgggccc ggggcgaccc gcgccgcggt ggagcacctg ggttatgtgc agatcgacac
     2221 catcaacgtg atcgagcgct gtcatcacca gatcctctac acgcgcatcc ccggataccg
     2281 gcgcgaccat ctgcgccagg cgcagacggt cgacaagacg gtgttcgaat actggacgca
     2341 tgcgctgtcc tacgtgccga cgcgggacct gcggttttac ctcggcgcca tgaagtggta
     2401 ccggcagcac cgcagcccct ggttcgccaa ggtgaaggag gaggacctgc ggcgggtgct
     2461 ggccctcatc agaaagcaag gcgccctcac gatccgggat atcgacgacg acgtgctggt
     2521 ggaaaaagac cacgcgtggg cgagccgcaa gccgtcaaag cgagtgctgc agctggcttt
     2581 ctaccagggc acggtgacca tcagcgagcg gaccggcatg ctgaagacct atgagctgac
     2641 gagccggcac ttcggctggc aaaggccgcc gaagcccgcg tcggaaagag aggtcctcga
     2701 ctatcagctc gaccgtgcgc tgcgagcgca gggcgtcgtg agcgtcgatt cggcctgcta
     2761 tctcgacgcg ccgcgcaagc cggccatgcg gcgattgatc gaggccaggc tgcgccgcaa
     2821 ggagctggtg agcgtcgagc tcgaaggggc cggaaagctg cagcact
//
LOCUS       DASQOP010000282        10472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1553484, whole genome shotgun sequence.
ACCESSION   DASQOP010000282 DASQOP010000000
VERSION     DASQOP010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10472
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1553484"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..976)
                     /locus_tag="VE030_11410"
     CDS             complement(74..976)
                     /locus_tag="VE030_11410"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HZF24058.1"
                     /translation="MNAVARKTWLEVALNGPWTRGRQPGIPVTVKDIVEQGIACVKAG
                     AAIVHLHAYDEASGRQTEDPDVYTRIIEGIRTRVDAIVYPTIPTSGLRGQPGEQTPQE
                     RFAHVEELARRRLIEWAAVDPGSTNFAHYDELREDKAGFVYLNSEDHIRHGLNLAMRY
                     GFHPAYAIYEPGFLRLGATLHWRSSCPRPIYRFMFSRGFTFGFPPEDYGVTAYLNLLD
                     QVAPGANWMIAGLDVDVLPLIPRAVMEGGHVRVGLEDAPLGSDKSNLQWVEESVQRIG
                     NAGGELATARDVRAALAPEDQETL"
     gene            complement(1003..1698)
                     /locus_tag="VE030_11415"
     CDS             complement(1003..1698)
                     /locus_tag="VE030_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZF24059.1"
                     /translation="MSENQLLFDIGPVASVPVIGRKERFPVHRIYCVGRNYAAHAREM
                     GKDPEKEPPFFFMKPADAVVPGGGQIHYPPGTQNLHHEIELVVAIQREGRNIAAAKAL
                     DHVYGYAVGLDMTRRDLQMKARDMGRPWDFGKSFDESAPITHIHPVASHGHHSTGNIW
                     LKVNGEMRQQADLADMIWNVPDTIAFLSEYYVLKPGDLIFTGTPAGVGAVKRGDQMVG
                     GVENLGELVVTVV"
     gene            complement(1701..2741)
                     /gene="gtdA"
                     /locus_tag="VE030_11420"
     CDS             complement(1701..2741)
                     /gene="gtdA"
                     /locus_tag="VE030_11420"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HZF24060.1"
                     /translation="MGQPMKDSRAERETYYRRIGRAHLTPLWESMSALVPKQPASPCV
                     PALWKYGEVRPWLMESGSLITAKEAVRRVLILENPGLPGQSSITHTLFAGWQLILPGE
                     VAPSHRHTQTALRLIVEGSGAYTAVDGERVTMHPGDFIITPSWTWHDHGNPAGEPVVW
                     LDGLDIPLVRFLDAGFAEEYPREQQEVVRPEGDAWRRYGNNLLPIEYRLQGASSPVFA
                     YPYARSRESLRQLGRVDKAHSCHGHKMEFVNPASGGPAMPSMSSFIQLLPRGFSGRPY
                     RSTDGTVFSVIEGNGRVVIGGETFEFTARDTFVVPSWHAYALYAEDDTVLFSFSDRPV
                     QRALGLFREELL"
     gene            complement(2933..3631)
                     /locus_tag="VE030_11425"
     CDS             complement(2933..3631)
                     /locus_tag="VE030_11425"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HZF24061.1"
                     /translation="MTATATIEVKTVAELAHQSEEYRTAVKKIVVSHAVNELYGAQVF
                     DEPAIALAPTPYAKWLTCRVAMEEYHHHVRFRGLAEEIGVAAEMLDPKVKKPLTIFDF
                     ELSTWPEFCVIKAIADYAEILQVEDLLHCSFHPLRNLARITMPEEKFHAKFGRDFCME
                     LIQSGEGRAVQDAINRYFPITPGFFGASNSKNNEMYLRWGLKSRTNDEMRADFLGRVG
                     ELVEKDFGLRLPAI"
     gene            complement(3642..4574)
                     /locus_tag="VE030_11430"
     CDS             complement(3642..4574)
                     /locus_tag="VE030_11430"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZF24062.1"
                     /translation="MNAGQALAGRVAIVTGGARGIGLAVVEELHRLGAAVLVADSGVS
                     ISGSEPDSSVAQTVAAKLGGNAAAFTEDMAATGAADRAVKLAVGKFGALDIVVNNAAI
                     LRDALIFKASRDNWEQVIANNLTAPFALLAAATPLMREQQKNGRAPGRIVNIVSSAGL
                     FGNFGQSAYASAKAGLVGLTRVVAMDMARSKVACNAIAPFAATRVTATIQPANEAQAA
                     YKERALRIPAHYVARLTALLCSNAHDITGQVFGVRGRELFLFSQARPVARTPMNPGTS
                     WGLEDLAKAMETGFAGKLTDLGTDLEFFNTEPLV"
     gene            complement(4571..5626)
                     /locus_tag="VE030_11435"
     CDS             complement(4571..5626)
                     /locus_tag="VE030_11435"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZF24063.1"
                     /translation="MSLTVTVADLNQSAPAGEGDTVLGALLAHGIGFAYSCQSGNCGT
                     CKCELVSGDILELEYSEHALSPDERGRGIVLACRSQMWSDVVVRRLDSEDFVVHPSRV
                     MRCRVAEIANLTHDILRLRLEIVTGGPFTFSAGQYAQLELPVAPGISRDYSMANRPDQ
                     PLLEFHVRVMPGGSVSHRIATALKVGDMVKVSGPMGTSYLRAQHPGPMLCIAGGSGLA
                     PVISIAGTALASGFAQPVHLYFGVRAERDVYFERELAGLQERHDNFRVYIVLSEPGAT
                     SSSGTLLPRRYGLVTDAVAADFADLGGFKAYFAGPPPMVDVATALVKTRAVGLRDIHA
                     DAFFPAAAETPKAAVAR"
     gene            5722..6174
                     /locus_tag="VE030_11440"
     CDS             5722..6174
                     /locus_tag="VE030_11440"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZF24064.1"
                     /translation="MKPAGARRKAASMGLLPGLIGYQLRLAQRAIFADFVETVDGAGI
                     SPGLFGILVIVDANPGLTQQALADAAHLDRSTVVTVLDKLEDRGLVERHAADRRSNGL
                     FLTRDGASLLRRLKRKVAQHERRVVHNLSARERNQLVALLQRILPGKR"
     gene            complement(6339..6761)
                     /gene="arfB"
                     /locus_tag="VE030_11445"
     CDS             complement(6339..6761)
                     /gene="arfB"
                     /locus_tag="VE030_11445"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HZF24065.1"
                     /translation="MNSDSRVPRVAENEVELRFVRASGPGGQNVNKVATAVELRFHIG
                     SSRSLTEDVKSRLRKLAGRRVSDQDVLVIDAQRFRSQEANRRDALARLGELVRAAWHR
                     PKSRIATRPTAASRRRRVEGKRVRAAVKRQRGRVSDSD"
     gene            7056..8801
                     /locus_tag="VE030_11450"
     CDS             7056..8801
                     /locus_tag="VE030_11450"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZF24066.1"
                     /translation="MKRWTYTGAAKVLSAAAFIGFATQVMAADVTWDRLVNADKDPNN
                     WIMYHQSFKGWHYSGLDQINASNVKDLKVAWLHSPAASKRGIQSFPLAVDGVVYYTSA
                     SGQVWALDGADGSVIWRYQAKIDTERAEGTFYNPYNRGLAIAYGKVYIGTVDGRMIAL
                     DAKTGKVVWDNMILSVEKGNKGFTGAPVVVKDKVIIGSNGGELSGCCGPIFAVNAQTG
                     EVAWQFDTIGGDERSRASWSNDSWKIGGGGGWMTGSYDAKTNTVWWGTANPAPDYDWA
                     GENWRTEGPRPGDNLYSSSVVVLDADTGELKSYFQEMPHDAWDFDSAPGEFMTFEKGG
                     KRYMLHPNKGGIIFVYNADQVGGGNQLKVENAYMLGETYNFIKGVDPKSGQLIGRREL
                     PEGKHTNVCPAIDGAISWNTGSYNPGTGLFYKIGQEWCFDIEVVKADRPADFSGQAYF
                     GASWTSTHPQGKQAYGHVTGRDPLSGKIKWRVEFKYPPLASLLSTKGNLVFVPGADGN
                     VHALDARTGKELWRHNNGIGHHGGVISYMAKGQQYIAVVAGWGSHVSGNYGPLFGHPF
                     TEMPTDAGQLIVFGL"
     gene            8890..9240
                     /locus_tag="VE030_11455"
     CDS             8890..9240
                     /locus_tag="VE030_11455"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZF24067.1"
                     /translation="MRVIRGRELLSIFAVVVGFVAAPAAANDPVDEAAAVVSEKYQLN
                     VKKLFATKCSWCHQGYGMKQADGPKLAGTPKTVEQVMKQINEGKSPMPGFRKQLSEKE
                     VQALAEYIKTLPAN"
     gene            complement(9303..9683)
                     /locus_tag="VE030_11460"
     CDS             complement(9303..9683)
                     /locus_tag="VE030_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24068.1"
                     /translation="MNGMWLAAIAVAANTCVGAAFADQLASLQGTWKGPWYIGMTSGL
                     ATMEIAADASGTIAFTNLENFGADPVALAKTSFDGKTFRFAASGTGGVAFSVAMQLGS
                     GGKQLRGNGKYGGFGARLELQRVD"
     gene            9814..10368
                     /locus_tag="VE030_11465"
     CDS             9814..10368
                     /locus_tag="VE030_11465"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZF24069.1"
                     /translation="MNFCSNCGARVERCVPPGDSLPRFVCAVCGTIHYENPKIVVGCI
                     PEWEDGILLCRRAIEPRHGLWTVPAGFMENGETTAQGAQRETLEEANARVEIVGLYAL
                     FNIPHINQVYMLFRARLLDLDFHAGAETLEARLFAESEIPWDTIAFATVRRTLQHYFS
                     DRRTGEFHFHMGTIEPMAKSKAEG"
BASE COUNT         2031 a         3321 c         3181 g         1939 t
ORIGIN      
        1 ctggagctca tgctttcctt gtggttctgt cggttgaaag gccgagatta tagggcgctg
       61 cgcatattgt tccctagagg gtttcctggt cctcaggggc cagcgcagcg cgcacatccc
      121 tcgccgtcgc cagttccccg ccggcgttgc cgatgcgctg caccgattct tccacccact
      181 gcaggttgct cttgtccgag cccagcggcg cgtcttccag gcccacgcgc acatgtccgc
      241 cttccatcac cgcacgcgga atcaacggga gcacgtccac gtccagcccc gcaatcatcc
      301 agtttgcgcc gggcgcgacc tggtcgagca gattgagata agccgtcacg ccgtaatcct
      361 cgggtggaaa gccgaaagtg aagccgcgcg agaacatgaa acggtaaatc gggcgcgggc
      421 aactgctgcg ccagtgcagc gtagcgccga gtcgcaggaa tccgggttcg taaattgcgt
      481 aggccggatg aaagccgtag cgcatcgcca gattcagccc atggcggata tggtcctcgg
      541 aattgaggta gacgaaaccg gccttgtcct cgcgcaattc gtcgtaatgc gcgaaattgg
      601 tcgatcccgg gtccaccgcg gcccactcga taagccggcg gcgcgcgagt tcctcgacgt
      661 gggcaaagcg ctcctgcggc gtttgttctc ctggctggcc ccttagcccg ctggtcggga
      721 tggtcggata gacgatcgcg tccaccctgg tgcggatacc ctcgatgatg cgcgtgtaca
      781 cgtccggatc ttcggtctgc cgcccgctcg cctcgtcgta ggcgtgcagg tgcacgatgg
      841 cagcacccgc cttcacgcag gcgatgccct gctcgacgat gtctttcacc gtgaccggaa
      901 tgccgggctg gcggccccgg gtccagggtc cgttcagtgc cacttccagc caggtcttcc
      961 gcgcgactgc gttcatgttc ttgttctgtc cgccgggaag actcaaacca ccgtgacgac
     1021 cagttccccc aggttttcca cgccgccgac catctgatcg ccgcgcttca ccgcgccgac
     1081 tcccgccggc gtgccggtaa aaatcaggtc gccaggcttg agcacgtaat attcggaaag
     1141 aaacgcgatg gtgtcgggca cgttccagat catgtcggcc aggtccgcct gctggcgcat
     1201 ttcgccgttg accttgagcc agatgtttcc ggtcgaatgg tggccgtggc tcgcgacagg
     1261 atggatatgc gtgatcgggg cggactcgtc gaaggacttg ccgaaatccc agggccggcc
     1321 catgtcgcgc gccttcatct gcaggtcgcg gcgggtcatg tcgagcccga ccgcatagcc
     1381 gtagacatgg tccagcgcct ttgccgccgc gatgttgcgc ccctcgcgct ggatcgccac
     1441 caccagttcg atctcgtgat ggagattctg ggtacccggc gggtagtgga tctgcccgcc
     1501 gccgggcacc accgcatccg caggcttcat gaagaaaaac ggcggctcct tctccggatc
     1561 cttgcccatc tcgcgcgcat gcgcggcgta attgcggccg acgcagtaaa tgcggtgaac
     1621 cggaaatctt tccttgcggc cgatgaccgg cacagatgcg accggcccga tgtcgaacag
     1681 cagctggttt tcgctcatgg tcagagcagc tcctcccgga acagcccgag cgcgcgctgc
     1741 accggccggt cggaaaagct gaacaacacg gtgtcgtctt ccgcgtaaag tgcataggcg
     1801 tgccaggacg gcacgacgaa ggtgtcgcgc gcggtgaact cgaaggtctc gccgccgatc
     1861 acaacccggc cgttgccttc gatcaccgag aacacggtgc cgtcggtgct gcggtaaggc
     1921 cggccgctga aacccctggg cagcaactgg atgaatgaac tcatgctcgg catcgccggt
     1981 ccgccgcttg ccggattgac gaactccatc ttgtgcccgt ggcagctgtg cgccttgtct
     2041 actcgaccca gttgacgcag gctctcgcgc gaacgcgcgt acggatacgc gaataccggc
     2101 gaggacgcgc cctgcaagcg gtattcgatc ggcaagagat tgttgccgta gcgccgccac
     2161 gcatcgccct ccggccgcac cacttcctgc tgctcgcgcg gatattcctc ggcaaagccg
     2221 gcgtcgagaa aacgcaccag aggaatgtcg agcccgtcca gccacactac cggctcgccg
     2281 gccgggttgc catggtcgtg ccaggtccac gacggcgtga tgatgaaatc tccgggatgc
     2341 atggtgacgc gctcgccgtc caccgcggta tacgcgccgg aaccttcgac gatcagtcgc
     2401 agcgcagtct gggtatggcg gtggctgggc gccacttcgc ccgggaggat gagctgccag
     2461 ccggcaaaaa gcgtatgggt gatcgaggac tgcccgggca ggccggggtt ctcgaggatg
     2521 agcacgcggc gcaccgcctc cttcgcggtg atcaggctgc cggattccat cagccacggc
     2581 cgcacttccc cgtacttcca cagcgccggc acgcacggcg aggccggctg cttcggcacc
     2641 agcgcgctca tcgactccca cagcggcgtc aggtgcgccc gcccgatgcg gcggtagtac
     2701 gtttcccgct ccgcgcggga atccttcatc ggttgtccca ttttttcctc cttaaccggc
     2761 catattcacc acagagacac agaggcacga agaaaagaat ttccgtacag aaaaaaacga
     2821 attcgacaaa gacggatgtt gccttgattt cgcgcgtgtt cggtcaattg cacgaatggg
     2881 atcttatatt gcatttctct gtgcctctgt gtctctgtgg ttagtgcgcc catcaaatcg
     2941 ccggcaatct caaaccgaaa tccttctcca ccagttcgcc cacgcgaccc aggaagtcgg
     3001 cgcgcatttc gtcgttggtg cggcttttca acccccagcg caggtacatc tcgttgttct
     3061 tcgaattgct ggcaccgaag aatcccggcg tgatcgggaa atagcggttg atcgcatcct
     3121 gcactgccct gccttctccg ctctggatca gctccatgca gaaatccctg ccgaacttgg
     3181 cgtggaattt ctcctcaggc atggtgatgc gcgccaggtt gcgcagcgga tggaaactgc
     3241 agtgaagcag gtcttccacc tgcaagatct cggcgtaatc cgcgatcgcc ttgatcacgc
     3301 agaactccgg ccaggtactg agctcgaaat cgaagatggt cagcggcttc ttcactttcg
     3361 ggtcgagcat ttccgcagca accccgatct cctccgccag ccccctgaac cgcacatggt
     3421 gatggtactc ctccatcgcc accctgcagg tcagccactt ggcgtaaggc gtcggcgcca
     3481 gcgcgatggc gggctcgtcg aacacctgcg cgccgtaaag ctcgttgacc gcgtgactga
     3541 cgacgatctt cttcaccgcc gtgcggtatt cctcggactg gtgcgccaat tcggctacgg
     3601 tcttcacttc gatggtggct gtggctgtca tggggtacac ctcaaacgag cggttcggta
     3661 ttgaagaatt ccaggtcggt gcccagatcg gtcagtttcc cggcaaaccc ggtttccatc
     3721 gccttcgcga ggtcttcgag cccccacgaa gttcccgggt tcatcggcgt ccgcgccacc
     3781 gggcgcgcct gggagaacag gaacagctcg cggccgcgca ctccgaacac ctgcccggtg
     3841 atgtcgtgcg cgttcgagca cagcaaggcg gtgaggcgcg cgacgtagtg cgccgggatc
     3901 ctgagcgccc tttccttgta cgccgcctgc gcctcgttgg caggctggat ggttgcggtc
     3961 acccgggtcg cggcgaacgg cgcgatcgcg ttgcacgcga ctttcgagcg tgccatgtcc
     4021 atcgctacga cgcgcgtcaa tcccaccagc ccggccttgg ccgacgcata ggccgactgg
     4081 ccgaaattcc cgaacagtcc ggcgctggag acgatgttga cgatgcgccc cggcgcccgg
     4141 ccgttcttct gctgttcgcg catcaacggc gtcgccgccg ccagcagcgc gaaaggtgcg
     4201 gtcaggttgt tggcgattac ctgttcccag ttgtcgcgac tggccttgaa aattaaggcg
     4261 tcgcgcagga tcgccgcatt gttcaccacg atatccagcg cgccgaattt ccccaccgca
     4321 agtttcaccg cccggtccgc ggcgccggtc gcggccatgt cttcggtgaa cgctgccgca
     4381 ttgccaccga gcttcgccgc gacggtctgt gcaacggaag aatcgggttc acttccggaa
     4441 atggatacac cgctgtcggc aaccaacacc gccgcaccga ggcgatgcaa ctcctccacg
     4501 accgccagcc cgatgccgcg tgcgcctccg gtgacgatcg cgaccctgcc agccagtgcc
     4561 tgtcccgcgt tcatctcgcc accgcggctt tcggcgtttc agcggccgcc gggaaaaacg
     4621 catcggcgtg aatgtcgcgc aaaccgaccg cgcgcgtctt caccaacgcg gtggccacgt
     4681 ccaccatcgg cggcggtccg gcgaaatagg ccttgaaacc gccgaggtcc gcgaaatccg
     4741 ccgccaccgc atcggtcacg agtccgtatc tgcgcggcag cagtgtgccc gaggaggacg
     4801 ttgcgccggg ttcggacaaa acgatataca ccctgaaatt gtcatggcgc tcctgcaacc
     4861 cggccagttc gcgctcgaag tagacgtcgc gctcggcgcg caccccgaaa tacagatgca
     4921 caggctgggc gaagccggag gcgagcgcag tcccggcgat ggaaatgacc ggcgccagcc
     4981 cgctgccgcc ggcgatgcat agcatcggcc cgggatgctg cgcccgcaga tacgacgtgc
     5041 ccatcggccc ggataccttg accatgtcgc cgaccttgag cgcggtggcg atgcgatggc
     5101 tcaccgaccc gccgggcatc acccgcacgt ggaactcgag caacggctgg tccgggcgat
     5161 tcgccatcga gtagtcgcgg ctgattccgg gcgccacggg cagctcgagc tgcgcatact
     5221 gccccgcgga aaaagtgaac ggcccgcccg tgacgatttc cagcctcagc ctgaggatgt
     5281 cgtgggtcag gttggcaatt tcggcgactc tgcagcgcat caccctcgac gggtgcacca
     5341 cgaagtcctc ggaatcgagg cgccgcacca ccacgtcgct ccacatttgc gagcggcagg
     5401 cgagcacgat gccgcggccg cgttcgtccg gggagagcgc gtgttcggaa tattccagct
     5461 cgaggatatc cccggatacc agctcgcatt tgcaggtgcc gcagttgccg gactggcagc
     5521 tgtaggcaaa gccgatgccg tgggcaagca gcgcaccgag cacggtatcg ccttccccgg
     5581 cgggcgcgga ctggttcaga tcggcgacgg taacggtgag gctcatcgag gctagcgatc
     5641 gcaggcgcca tgggctaata tccttaaagt atgggaacca aactgttgga tgtcaaacaa
     5701 tcggggcggc cgcgcagggg aatgaaaccg gcgggcgcaa ggcgcaaggc cgccagcatg
     5761 ggcctgctgc ccggcctgat cggctaccag ttgcggctcg cgcagcgcgc gatctttgcc
     5821 gatttcgtag agaccgtgga cggcgcagga atttcccccg gcttgttcgg aatcctggtg
     5881 atcgtcgacg ccaacccggg actcacccag caagcgctgg ccgatgccgc acacctggat
     5941 cgctccaccg tcgtgaccgt gctcgacaag ctggaagacc ggggactggt ggagcgccac
     6001 gccgccgatc gccgcagtaa cgggctgttc ctgacccgcg acggcgccag cctgctgcgc
     6061 cggctgaagc gcaaagtcgc ccagcacgaa cgccgcgtgg tgcacaacct cagcgccagg
     6121 gagcgcaatc aactggtcgc gctcctgcaa cgcatcctgc cgggaaaaag ataattaaga
     6181 ggcagtgatt gagcgatgag ggatgagtgc tgagtgctga gtgctgagtg ctgagtgctg
     6241 accaaaagtg tagtcggact tgccgctctt tgcgtccttt gcgttccttt gcgtcctttg
     6301 cgtcaacgct cttatcgccg gccggaggcc ggctgatctc aatccgaatc ggaaacccgg
     6361 ccgcgctggc gcttcacagc ggcgcgcacg cgctttccct ccactcttcg acgccttgat
     6421 gccgcagtcg gcctagttgc gatccgcgac ttgggccggt gccaagcggc gcgcaccagc
     6481 tcgcccaggc gcgccaacgc atcgcgccgg ttggcctcct gactgcgaaa tcgctgcgcg
     6541 tcgatcacca gcacgtcctg atcgctgacc cgccttccgg ccagtttgcg cagccggctc
     6601 ttgacatctt cggtcaatga cctgctcgag ccgatatgga agcgcagctc gaccgcggtg
     6661 gcgaccttgt tcacgttctg gccgccgggg ccggacgcgc gaacgaagcg cagctccact
     6721 tcgttctcgg caacgcgggg aactctgctg tccgaattca tcgatgcgtg tccacaatca
     6781 gcttcgaaat ggcgtttcag gagtcaaaat catgcgttta tttgccgcgc tgcaacagcc
     6841 ggaaacgccc gtccggcgcg gacttcaggg gtttcaaaaa attccggtca gcgcttgcaa
     6901 atgaatagta ggaggaatac atttgctgcg ggttttccca ccgtacacaa ctcgaaacaa
     6961 gcagttaaca agcgaatgcg taacgggggc gatctgattt ccctacagtt ttgcccaatt
     7021 ccagaaattg aaaccgagat aaacgggaga gagttatgaa acgatggacc tatacagggg
     7081 cggcaaaagt cctgtccgca gccgcattca tcgggttcgc tacccaggtc atggcagcag
     7141 atgttacctg ggaccgcctg gtgaacgccg acaaggaccc gaacaactgg atcatgtatc
     7201 accagtcgtt caaaggctgg cactatagcg gcctcgatca aatcaacgcc agcaacgtca
     7261 aggacctgaa ggtggcctgg ctgcacagcc cggctgcttc caaacgcggc attcaatcct
     7321 tccccctcgc agtcgacggc gtcgtgtatt acaccagcgc atccggccag gtgtgggccc
     7381 tcgatggtgc ggacgggtcg gtgatctggc gctatcaggc caagatcgac accgagcgtg
     7441 cagaagggac tttctacaac ccgtacaacc gcggtctggc cattgcctac ggcaaagtct
     7501 acatcggcac cgttgatggc cgcatgatcg cgctcgacgc caagaccggc aaagtagtct
     7561 gggacaacat gatcctcagc gtcgaaaaag gcaacaaggg cttcaccggc gctcctgtgg
     7621 tggtgaagga caaggtcatc atcggttcca acggtggcga gttgtccggc tgctgcggcc
     7681 cgatcttcgc ggtcaacgcg cagacgggag aggtggcctg gcagttcgac accatcggcg
     7741 gcgacgagcg ttcccgagct tcctggagca acgactcctg gaagatcggc ggtggcggcg
     7801 gctggatgac cggttcgtac gatgccaaga ccaacaccgt gtggtggggt accgccaacc
     7861 cggctcccga ctacgactgg gcaggagaga actggagaac cgagggaccg cgtccgggtg
     7921 acaacctgta cagctcctcg gtcgtggttc tcgatgccga caccggcgag ctgaaatcgt
     7981 acttccagga aatgcctcac gacgcctggg acttcgacag cgcgccgggc gagttcatga
     8041 ccttcgagaa gggcggcaag cgctatatgc tgcacccgaa caagggcggc atcattttcg
     8101 tttataacgc tgaccaggtt ggcggtggca accagctgaa ggtggaaaac gcttacatgc
     8161 tgggtgagac ctacaacttc atcaagggtg tcgatccgaa gagcggccaa ttgatcggcc
     8221 gtcgcgagct ccccgaaggc aagcacacca acgtgtgccc ggcgattgac ggtgcgatca
     8281 gctggaacac cggctcctac aaccccggta ccggcctgtt ctacaagatc ggccaggagt
     8341 ggtgcttcga catcgaagtc gtgaaggctg atcgcccggc cgacttctcc ggtcaagcct
     8401 acttcggcgc ttcgtggacc tctacgcacc cgcaaggcaa gcaagcctac ggccatgtta
     8461 ccggccgtga cccgctgtcc ggcaagatca agtggcgcgt cgagttcaag tatccgccgc
     8521 tggcctcgct gctttccacc aagggcaacc tggtgttcgt gcccggcgcc gatggaaacg
     8581 tccatgcact cgacgcccgc accggcaagg agctgtggag gcataacaac ggcatcggcc
     8641 accatggcgg cgtgatctcc tacatggcca agggccagca gtacatcgcc gttgtcgccg
     8701 ggtggggcag ccatgtgtcg ggcaactacg gcccgctgtt cggccatccg ttcaccgaga
     8761 tgccgaccga cgccggtcag ctgatcgtgt tcggcctgta aaccaataaa aacattcagt
     8821 cgttcattgc gggcggggga gcaatccctc gcccatttag tttgtcgaac ttgcgacgga
     8881 gagcttcagg tgagagtcat acgcggcaga gaattacttt ccatcttcgc ggtcgtcgtg
     8941 ggatttgttg cagcgcccgc cgcggcaaat gatccggtcg acgaggccgc ggccgtggtc
     9001 agcgagaagt accaattgaa tgtcaagaaa ctgttcgcga cgaaatgcag ctggtgtcat
     9061 cagggctacg gcatgaaaca ggccgatggc ccgaagctcg ccggcacgcc gaagacggtc
     9121 gaacaagtaa tgaagcagat caacgaaggg aaatcgccga tgcccggctt caggaagcaa
     9181 ctcagcgaga aagaggtaca agcgctcgcc gagtacatca agacgttacc ggcgaactga
     9241 ttgctgcggg cgatctgaag gaaaaacgaa gggcggtctt taccgccctt tcttatgcca
     9301 aatcagtcca cgcgctgcag ttccagacgc gcgccgaagc ccccgtattt gccgttgccg
     9361 cgcaattgct taccgcccga accgagttgc atggcaaccg agaatgcgac gccgccggta
     9421 ccggaagctg caaacctgaa tgtcttgccg tcgaaagaag tcttggcaag cgcaaccgga
     9481 tctgcgccga agttttcgag attggtgaat gcaatcgtgc cggaagcgtc cgccgcgatc
     9541 tccatcgtcg ccaggccgct ggtcatgccg atgtaccacg gacctttcca agtgccctgc
     9601 aacgaagcca attgatccgc gaacgcggcg ccaacgcaag tgttcgccgc cacagcgatt
     9661 gccgccagcc acattccatt catgggttcc tccagagcgt ccggacacga atcccggcag
     9721 tccggtgatt ctagcaatcc cggcccgcgc ccgttatggt gcctgctctg cgagccctta
     9781 taatgaaggc aggcgcatat aacttccggg ccaatgaact tctgcagcaa ttgcggtgct
     9841 cgcgtggagc gctgcgtacc tccaggcgac agtctccccc gcttcgtttg cgccgtttgc
     9901 ggaaccattc attacgagaa cccgaagatc gtggtgggct gtatccccga atgggaagac
     9961 ggcattctgc tgtgtcgccg cgccatcgaa ccgcgccacg gattgtggac ggttccggcc
    10021 ggattcatgg agaacgggga aaccaccgcg caaggcgcgc agcgcgaaac tctcgaagag
    10081 gccaatgcgc gcgtggaaat tgtgggcctt tacgcactgt tcaacattcc ccacatcaac
    10141 caggtgtaca tgctgttccg tgcccggctg ctcgacctcg attttcacgc gggcgcggaa
    10201 acgctggaag cacggttgtt cgctgaaagc gaaatcccgt gggacacaat cgctttcgcc
    10261 acggtgcggc gcacgctgca gcactatttc agcgaccgcc ggaccgggga gttccatttc
    10321 catatgggaa ccatcgagcc gatggcgaag agcaaggcgg aaggatgagg gatgaggaat
    10381 gaaggatgaa gggtgaaggg tgaaggccgc aataggaacc ggtatggtac atatggcgga
    10441 aaataggcac aattgaagtt catacgtatt cc
//
LOCUS       DASQOP010000283         2145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1150214, whole genome shotgun sequence.
ACCESSION   DASQOP010000283 DASQOP010000000
VERSION     DASQOP010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150214"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VE030_11470"
     CDS             complement(<1..531)
                     /locus_tag="VE030_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HZF24070.1"
                     /translation="MTAAFVHYRPGMLPWTVSEEEERRFRRILKRVLLVCILFSLIMP
                     WLPVPKVDRTKEEELPPRLAKLLLERQTAPPPTVVRKPEPEVAAKPQPEVAKPEPKKP
                     PVAEARKPEPAKAPGERLAAARRRASGVGLLAMKDELADLRGAPVAAQFKKDIKPGPG
                     VGSGSGPGVGAGKSPGL"
     gene            complement(531..1025)
                     /locus_tag="VE030_11475"
     CDS             complement(531..1025)
                     /locus_tag="VE030_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZF24071.1"
                     /translation="MKLSPLQKRAERHARNKFSVDLNLVSLIDIFTILIFFLLANASE
                     VEVLPSTKAVRLPESTAEKTPKETLVVLVSNQDIVVQGRKVSSVSEAMKADGDLIAPL
                     KAELDYHAGRQLIRKEKEARNKAITIMGDKEISYRLLRKIMVTCARANYSDISFAVIQ
                     KGKA"
     gene            complement(1022..1534)
                     /locus_tag="VE030_11480"
     CDS             complement(1022..1534)
                     /locus_tag="VE030_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZF24072.1"
                     /translation="MSSRVRRLRKHSLGLDITAFMNMIVVLVPFLLTTTVFSRLAVLE
                     LSLPAQSSGFDKLKGDLQLEVVIRGDALEIRDRIGGLIQRVQNTSKGYDFRTLSASMQ
                     QLKTRFPDKVEAAILAEPDTSYDILVQTMDAVRVARTSGSGHAELFPEISIGDAPVAG
                     GAPAKPGKRS"
     gene            complement(1531..>2145)
                     /locus_tag="VE030_11485"
     CDS             complement(1531..>2145)
                     /locus_tag="VE030_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HZF24073.1"
                     /translation="SGIFIYPSILIMAVGLAIALERYFYLSHARRSNRRTWDELLPLL
                     QGGEFQQAYSLTAKSKTAIGKIVNSGLRRVKSARRREDLDAAMEEGMMEIVPRLEKRT
                     HYIATFANVITLVGLLGTIIGLIKAFTAVAQVNPAEKAELLSASISIAMNNTAFALMV
                     AIPFLLIHAFLQARTSEIVDSLEVAKISFLNLVERVKAEPAPAK"
BASE COUNT          368 a          705 c          663 g          409 t
ORIGIN      
        1 aagccccggg ctcttgccgg cgcccacgcc cggcccggaa ccggacccga cgccgggccc
       61 cggcttgatg tcttttttga actgcgcagc aaccggcgcg ccgcgcagat cggccagttc
      121 gtctttcatc gccagcaaac ccacgccaga ggccctgcgg cgggcggccg ccagtcgttc
      181 ccccggcgcc ttggccggct cgggcttgcg cgcttcggcg accggcggct tcttcggttc
      241 cggcttcgca acttcgggct ggggcttcgc cgccacttcg ggttccggtt tcctgaccac
      301 ggtcggcggc ggagccgttt ggcgctcgag cagcagcttg gcgagtcgcg ggggcaactc
      361 ctcctccttg gtgcgatcca ccttcgggac cggcaaccac ggcatgatca agctgaagag
      421 aatgcaaacg agcagcacgc gcttgaggat gcggcggaag cgccgttctt cctcctcgga
      481 aaccgtccac ggcagcatcc cgggacggta gtgaacgaag gccgcggtca ttacgccttg
      541 cccttctgaa tcacggcaaa ggagatgtcg ctgtagttgg cgcgcgcgca agtcaccatg
      601 atcttgcgca gcagccggta cgaaatctcc ttgtctccca tgatcgtgat ggccttgttc
      661 cgggcctcct tttccttgcg gataagctga cgcccggcgt gatagtcgag ctcggccttg
      721 agcggcgcga tgagatcgcc gtcggccttc atcgcctcgg acaccgagga caccttgcgt
      781 ccctggacca cgatgtcctg gttgctcacc agcacgacca gggtttcctt cggagtcttc
      841 tcggccgtgg actccggcaa cctcaccgcc ttggtgctcg gcagcacctc cacctccgat
      901 gcattggcga ggaggaagaa gatcaggatg gtgaaaatgt ctatcagcga aaccaggttg
      961 aggtccaccg agaacttgtt gcgcgcgtgc cgctcggccc gcttttgcaa cggcgagagc
     1021 ttcatgacct tttccccggt ttcgccggcg ccccgcccgc aacgggcgca tcgccgatgg
     1081 agatctccgg gaacagttcg gcgtgcccgg acccggaggt tctcgccacg cgcacggcat
     1141 ccatggtctg caccaggatg tcgtaggacg tatcgggttc ggcgagaatc gccgcttcga
     1201 ccttgtccgg aaagcgcgtc ttgagttgct gcatcgacgc cgacagagtg cggaagtcgt
     1261 accccttgct cgtgttctgg acgcgctgaa tcagcccgcc gatgcgatcc cggatctcga
     1321 gcgcgtcgcc ccggatcacg acctcgagct gcagatcgcc cttcagcttg tcgaacccgg
     1381 aggattgcgc cggcaggctc agctccagca ccgccaggcg cgaaaacacc gtcgtcgtga
     1441 gcagaaacgg caccagcacc acgatcatgt tcatgaacgc ggtgatgtcc agccccaacg
     1501 aatgcttgcg caggcgtcgg acgcgggagc tcatttggcg ggcgccggtt cggccttcac
     1561 gcgctcgacc agattcagga aactgatctt ggccacctcc aggctatcca cgatctcgct
     1621 ggtgcgcgcc tggaggaacg cgtggatgag caggaacggg atcgccacca tcagcgcgaa
     1681 cgccgtgttg ttcatcgcga tcgagatgct ggccgagagc agctcggcct tctccgccgg
     1741 attcacctgc gcaacggctg taaaggcctt gatcaggccg ataatggtgc cgagcagacc
     1801 caccagcgtg atcacgttgg cgaaggtggc gatgtagtgg gtgcgcttct ccagccgcgg
     1861 cacgatctcc atcatgccct cttccatggc ggcatcgagg tcctcgcgcc ggcgcgcgct
     1921 cttcacgcgc cgcaggccgc tgttcacgat cttgccgatg gcggtcttcg atttggcagt
     1981 cagggaatag gcctgctgaa actcgcctcc ttgaagcagc ggcaacaact cgtcccaggt
     2041 tctgcgattg cttctgcgcg cgtggctgag atagaaatag cgttccagcg cgattgcaag
     2101 gccgaccgcc atgatcagaa tgctgggata gatgaaaatc ccgct
//
LOCUS       DASQOP010000284         6153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_142368, whole genome shotgun sequence.
ACCESSION   DASQOP010000284 DASQOP010000000
VERSION     DASQOP010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6153
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142368"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..377)
                     /locus_tag="VE030_11490"
     CDS             complement(27..377)
                     /locus_tag="VE030_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24074.1"
                     /translation="MDRILDTTFETRRAAIWKVNRRWVFASAAAVALVVMFADLANAP
                     RDLSWALALGAGALLAVTLTRIVRALNVLYRCPNCGVLPYQTLSEYKCGGLGPTRSNF
                     MSPTHCPKCGTRLR"
     gene            376..501
                     /locus_tag="VE030_11495"
     CDS             376..501
                     /locus_tag="VE030_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24075.1"
                     /translation="MGRSGYASDTPDVLILRRYRGVLKVSETDPPVTSIEDSAAG"
     gene            complement(525..1364)
                     /locus_tag="VE030_11500"
     CDS             complement(525..1364)
                     /locus_tag="VE030_11500"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013207018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZF24076.1"
                     /translation="MADSPRNVGQQDDLSGPARPWRPAIVVQLTILIHLAALVALLFR
                     LAWWPWIVAALVANHVALGLIGMWPRSRLLGDNMLRLPDTAARRGEIAITFDDGPHPE
                     VTPKVLDILDRYGAKASFFCVGEKAASHPDIVMEIVKRGHTIENHSMRHPGLFGFYGP
                     MALQREIGAAQSVLGGLAGRPPRFFRAPMGIRNPMLDPVVARMGLHYISWTRRGFDTV
                     ARDPAAVLKRLIDSLAGGDILLLHDRPTVHGEPIVLIVLPQLLEKIHSAGLKPVSLTM
                     AMR"
     gene            complement(1426..1566)
                     /locus_tag="VE030_11505"
     CDS             complement(1426..1566)
                     /locus_tag="VE030_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24077.1"
                     /translation="MKQANQQWIERRNAAVLDEGLGIIEKSLAEVAQASRMPKEPAAA
                     AR"
     gene            complement(1614..3374)
                     /locus_tag="VE030_11510"
     CDS             complement(1614..3374)
                     /locus_tag="VE030_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HZF24078.1"
                     /translation="MNKPAAPTAQVTNFIRNIIEADLAKGTYASRRWGGKPGPASTHA
                     NALPDPAKIRTRFPPEPNGYLHFGHAKSICLNFGVALQYGGRCHMRFDDTNPVTEDTE
                     YVDSILDSVKWLGFDWGPHLYYASDFFDWLYRFAEAFVEHGLAYVDSLSADEMREYRG
                     TLTEAGKHSPYRDRPAAENLDLFRRMKAGEFPDGAHVLRLKIDMSSPNINLRDPVIYR
                     IRHAHHHRTGDKWCVYPMYDYTHCISDALENITHSLCTLEFEDHRPLYDWVLERLADF
                     GILAKPLPHQYEFARLNLTYVVLSKRKLIQLVEEKHVDGWDDPRMPTLVAARRRGFTP
                     EGFRLFAERIGVSKSDSWIDMSVLEDCMREVLNESAPRRIAVLEPVRLVIDNYPAGKE
                     EMCELPNHPQKPEWGKRAVPFARELWIEREDFMETPSKGFFRLFPGNEVRLRYGFVVK
                     CIGADKDAGGNVTAVHCEIYPDSKSGTPGADKYKVKGNIHWVSAQHACKAEVRLYDRL
                     FAVTHPGSERDFLADINPHSKTVLHAYCEPSVREAKPEERFQFERHGYFIADLKDSKP
                     GAPVFNRTATLRDSWVKKAS"
     gene            3520..5034
                     /locus_tag="VE030_11515"
     CDS             3520..5034
                     /locus_tag="VE030_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24079.1"
                     /translation="MHSSHLRAPFALLALTAALTAFADDGGNHALTIYSTATPGAVAP
                     DMTRNGGSGYSVPGYATVRHEREIALNAGRNTVRFTDVAGLIDPTTVSFASLTDTSGT
                     RVMEQNFQFDLVSPEKLLRRFIDRDIAVERVLGDRIETLEGTLLSTQGGIVIRATDGT
                     IRTLPYNAGVKLPSLPGGLITRPTLVWDVAAKQAGKHRTRVAYQTGGITWWADYNLTF
                     SEGGDANSCKLDVGAWVSILNQSGASYPDARIKLVAGDVHRAAHMGQAVPMVAKRAMA
                     DIAEEKSGFEEKTFFEYHLYTLGRPSSLPDNSTKQIELFPKVADVTCEKTLVYYGQDG
                     QWYGGGPMTDRNYGITGNKKVDTYLSFRNKAENGMGVPLPSGRVRVAKLDTADGSLEF
                     IGEDRIDHTPKNETVLLKLGSAFDVVGERRQVDFKVDTNRNTMTEEIEVKIRNRKDEP
                     VTVIVKENLYRWTNWKITQAIQPWEKQDSRTIHFPVKVAADAEALVRYTVRYTW"
     gene            complement(5195..>6153)
                     /locus_tag="VE030_11520"
     CDS             complement(5195..>6153)
                     /locus_tag="VE030_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZF24080.1"
                     /translation="FLFLCVLCVLCGSSLSSAVDGYPSKPIRIIVPYTPGGFNDTLAR
                     TLGQKLNEKWGQPVMVDNRPGGGTTIGTSLVAKAPADGHTLLVVSFAFGVNPSLYTLL
                     PYDTQRDFAPVVLAAGTPNILVVNPELPVKSVRDLITLAKSKPGKLNYATAGSGSSNH
                     LSMEMFKSMAGVDIVHVPYKGSAPAVTDLIGGQVDVMFDNVPNVLQHVKAGKLRGLAV
                     SSRERSPFAPDLPTVAEAGLPGFDVSVWFGVVAPSGTPKAVVSQLNGEINRILKLPGI
                     VELFHKQGVEPLGGTPDAFASFLRDQTVKWAKVVKQSGAKAE"
BASE COUNT         1164 a         1879 c         2016 g         1094 t
ORIGIN      
        1 ggcgttcaac tattatgaat ggaaattcat ctgaggcgcg tgccgcactt cgggcagtgg
       61 gtcgggctca tgaaattgga acgggtgggg cccaggccgc cgcacttgta ttcgctgagc
      121 gtctgatacg gcagcacgcc gcagttgggg cagcggtaca gcacgttcag cgcccttacg
      181 atgcgggtga gcgttaccgc aagaagggct ccggcgccga gggcgagcgc ccaggagagg
      241 tctcgcggcg cgttggcgag gtcggcgaac atcaccacca gcgcgaccgc ggcggccgac
      301 gcgaacaccc agcgccggtt gaccttccag attgcggcgc ggcgtgtttc gaatgtcgta
      361 tccagaattc ggtccatggg taggtccggc tacgcgagcg atacgcccga tgtcctcatt
      421 ctgcgacgtt accggggcgt tctcaaggtc agcgaaaccg atccgccggt tacgtccatc
      481 gaggattccg ccgccgggta ggggcgcgcg aacctcgggc tgcatcagcg cattgccatc
      541 gtcagcgaca ccggcttcag acccgccgaa tgaatttttt ccagcagctg cggcaacacg
      601 atgagcacga tcggttcccc gtgcaccgtc ggacggtcgt ggagcagaag aatgtcgccg
      661 ccggccaggc tgtcgatcaa tcgcttcaga acggccgccg gatcgcgggc gacggtgtcg
      721 aaaccgcgcc gggtccagga aatgtagtgc agccccatgc gcgccaccac cggatcgagc
      781 atcggattcc tgatgcccat cggcgcgcgg aagaaacgcg gcggacgccc ggcaaggccg
      841 ccgagcaccg actgcgctgc accgatttcc cgttgcagcg ccattggccc gtaaaaaccg
      901 aacaaaccgg ggtgacgcat gctgtggttc tcgatggtgt ggccgcgttt cacgatttcc
      961 atgacgatgt cgggatgcga ggcagccttc tcgccgacgc agaagaaact cgccttggcg
     1021 ccgtagcggt cgagaatgtc caagaccttc ggcgtgactt ccgggtgcgg gccgtcgtcg
     1081 aacgtgatgg cgatttctcc gcggcgcgcg gccgtatcgg gcagccgcaa catgttgtcg
     1141 ccgagcaggc ggctcctggg ccacatcccg atcagtccca atgccacgtg attggcaacg
     1201 agcgcggcca cgatccacgg ccaccacgcc agccggaaaa gcagcgcgac gagtgcggcg
     1261 aggtgaatca gtatggtcag ctggaccacg atggccgggc gccatggccg ggccgggccg
     1321 gaaaggtcgt cctgttgtcc gacgttgcgc ggagagtcag ccatcctggg cgccagtgcg
     1381 gtgtctgcgc ggctacgacc ggctggcgcg aaggatgacg acccgttacc gcgccgcggc
     1441 cgcgggttct ttcggcatgc ggctcgcctg cgcgacctcg gcaagcgatt tttcgatgat
     1501 gccgaggcct tcgtcgagca cggcggcgtt gcggcgttcg atccactgct gattggcctg
     1561 cttcatggtt gcctccacct caccccagct ctctctccag agcggagagg gagtcaggac
     1621 gcttttttga cccaggaatc gcgcaaagtc gccgtccggt tgaagaccgg tgcgccgggt
     1681 ttcgaatctt tcaagtccgc tatgaagtaa ccgtggcgct cgaactggaa acgctcctcg
     1741 ggcttcgcct ccctgaccga aggttcgcaa taggcatgga ggacggtctt ggagtgcgga
     1801 ttgatgtcgg cgaggaagtc gcgttccgat ccggggtgag tcaccgcgaa caggcggtcg
     1861 tagagccgca cttcggcctt gcaggcatgc tgcgccgaca cccagtggat gttgcccttc
     1921 accttgtatt tgtccgcgcc cggcgtgccc gacttggaat ccggatagat ctcgcagtgc
     1981 accgcggtga cgttgccgcc ggcgtccttg tcggcgccga tgcacttgac cacgaagccg
     2041 tagcgcagcc tcacctcgtt gccgggaaac aggcggaaga aacccttgga cggcgtctcc
     2101 atgaaatcct cgcgctcgat ccacagctcg cgcgcgaacg gcacggcgcg cttgccccac
     2161 tccggcttct gcgggtggtt gggcagttcg cacatctctt ccttgccggc cgggtaattg
     2221 tctatcacca gtctgaccgg ttcgagcacg gcaatgcggc gcggcgccga ttcgttcagc
     2281 acctcgcgca tgcagtcctc gagcacgctc atgtcgatcc acgagtcgga cttggagacg
     2341 ccgatacgct ccgcgaacag ccggaaacct tccggcgtga agccgcgacg gcgcgcggcg
     2401 accagggtgg gcatgcgcgg atcgtcccag ccatccacgt gcttttcctc gaccaactgg
     2461 atcagcttgc gcttggacag gaccacgtag gtgagattga gccgggcgaa ctcgtactgg
     2521 tgcggcagcg gcttggcgag tatgccgaaa tccgcgagcc tttccagcac ccagtcgtag
     2581 agcggccggt gatcctcgaa ctcgagcgta cagagcgagt gggtgatgtt ctcaagcgcg
     2641 tccgagatgc agtgggtgta gtcgtacatc gggtaaacgc accacttgtc gccggtgcgg
     2701 tgatggtgcg catggcggat gcggtagatc accggatcgc gcaggttgat gttgggggaa
     2761 ctcatgtcga tcttcagacg cagcacgtgc gcgccgtccg gaaactcgcc tgccttcatg
     2821 cgccggaaca gatcgaggtt ttccgccgcc gggcggtcgc ggtaggggct gtgcttgccc
     2881 gcttcggtga gcgtgccgcg gtactcgcgc atctcgtcgg cggacagcga atcgacgtag
     2941 gccaggccat gctcgacgaa ggcctcggcg aaccggtaca gccagtcgaa gaagtcggac
     3001 gcgtaataca ggtgcgggcc ccagtcgaat cccagccact tcaccgaatc gaggatggaa
     3061 tcgacgtact cggtgtcttc ggtgaccgga ttggtgtcgt cgaagcgcat gtggcagcgg
     3121 ccgccgtact gcagcgccac cccgaagttc aggcagatgg acttggcgtg gccgaaatgc
     3181 aggtagccgt tgggctcggg cggaaaacgg gtgcggatct tggccggatc gggcagtgcg
     3241 ttggcgtgcg tcgacgcggg gcccggtttg ccgccccatc tgcgggaggc gtaagtgccc
     3301 ttggcgagat cggcctcgat gatgttgcgg atgaagttgg tgacctgggc ggtcggcgct
     3361 gccggcttgt tcacgctgga tgacggaata aagtaggagg ctgtgcagac gcaatattaa
     3421 tgcctcgcag cttgtcgtgg gcgtaaacct gctaacccca ttttgagccg gcgggcgttt
     3481 acatgagcgc accccccttc caataggaaa ggaacgttca tgcactcttc tcatttacgc
     3541 gcgccgttcg cgctgcttgc actgaccgct gctctgactg cctttgccga tgacggcggc
     3601 aaccacgccc ttaccatata cagcaccgca acacccggcg cggtggcacc ggacatgaca
     3661 cgcaatggcg gtagcggcta cagcgtgccc ggctacgcca ccgtgcgcca tgagcgcgag
     3721 atcgcgctca acgccggtcg aaacaccgtg cgcttcaccg acgtcgccgg tctgatcgac
     3781 ccgaccacgg tgtcgttcgc ctcgctcacc gacacgtccg gcacgagggt gatggaacag
     3841 aacttccagt tcgacctggt cagcccggaa aaactgttac ggcgcttcat cgaccgggac
     3901 atcgcagtgg agcgcgtgct tggcgaccgc atcgagactc tggaaggcac gctgctctcc
     3961 acccagggcg ggatcgtgat ccgagcaaca gacggaacca ttcgcacgct gccgtacaac
     4021 gccggggtga aactgccgtc gctgcctggc gggctgatca cgcggccgac gctggtgtgg
     4081 gacgtggccg cgaaacaggc cggaaagcac agaacccggg tggcctatca gaccggcggc
     4141 attacctggt gggcggacta caaccttacg ttctccgagg gcggcgacgc gaattcgtgc
     4201 aagctcgatg tcggtgcgtg ggtgagcatc ctcaatcagt ccggcgccag ctacccggac
     4261 gccaggatca agctggtcgc cggcgatgtg caccgggccg cgcacatggg acaggccgtg
     4321 cccatggtcg ccaagcgggc gatggcggac atagcggaag agaagtccgg tttcgaggaa
     4381 aagacgttct tcgagtatca cctctacacg ctgggacgcc ccagctcgct tccggacaac
     4441 tcgaccaaac agatcgaact gttcccgaaa gtcgccgatg tcacctgcga aaagacgctg
     4501 gtgtactacg ggcaggacgg gcagtggtac ggcggcgggc cgatgaccga ccgcaactac
     4561 ggcattaccg gcaacaagaa agtcgacacc tatctcagct tcaggaacaa ggccgagaac
     4621 ggcatgggcg tgccgttgcc ttcgggccgg gtgcgggtcg ccaaactcga cacggcggac
     4681 ggcagcctgg agttcatcgg cgaggaccgt atcgatcaca cccctaagaa cgaaaccgtg
     4741 ctgctcaagc tcgggagcgc tttcgacgtg gtcggcgagc gccggcaggt ggacttcaag
     4801 gtggacacaa atcgcaacac catgaccgaa gagatcgaag tcaagatccg caaccgcaag
     4861 gacgagccgg tcacggtgat cgtgaaggag aacctctacc gttggaccaa ctggaagatt
     4921 acgcaggcga tccagccgtg ggaaaaacag gactcgcgca cgattcactt tcccgtgaaa
     4981 gtggcggcgg acgccgaggc cctggtgcgc tacacggtgc ggtatacgtg gtgacggcaa
     5041 gccgggcgga ggctggactc aaaagcgttg acgcagagga cgcagagaat aagagaaagg
     5101 acgcagcgga aattcgaata ctgcggattt tggtttgacg ccgatgccgc gacccgattc
     5161 ccaaacacgg cagaaatacc ggcttatgcg acgttcattc cgcctttgcg ccggactgtt
     5221 tcaccacctt tgcccatttg acggtctgat cgcgcaggaa tgaggcgaac gcgtccggcg
     5281 tgccgccgag cggctcgacg ccctgtttgt ggaatagctc gacgatcccg ggcagcttga
     5341 ggatgcggtt gatttcgccg ttgagctgcg agaccacggc tttcggcgtg cctgatggcg
     5401 cgaccacgcc gaaccagacc gagacgtcga agccgggcaa accggcctcg gcgacggtcg
     5461 gcaggtccgg cgcgaagggc gagcgctccc tgctcgacac cgccagtcca cgcagcttgc
     5521 cggccttcac gtgttgcagc acgttgggca cgttgtcgaa catgacgtcc acctggccgc
     5581 cgatcaggtc ggtgactgcg ggggcgctgc ccttgtaagg aacgtgcacg atgtcaacgc
     5641 cggccatgct cttgaacatt tccatcgaca ggtggttgga cgaaccgctt cctgcggtcg
     5701 cgtagttgag tttcccgggc ttcgattttg cgagcgtgat caggtcccgc accgatttca
     5761 ccggcagttc cggattgacc accaggatat tcggcgtccc ggcggcgagc acgaccggcg
     5821 cgaaatcccg ttgcgtgtcg tagggaagca gcgtgtacag actcgggttg actccgaaag
     5881 cgaaggaaac gaccagcagc gtgtggccat cggcgggcgc tttcgctacg aggctggtac
     5941 cgattgtggt gccgccgccc gggcggttgt ccaccatcac cggctggccc cacttttcgt
     6001 tgagcttctg tccgagcgtg cgcgccagtg tgtcgttgaa cccgccgggc gtgtacggaa
     6061 cgatgatgcg gatcggcttg ctcggatagc cgtctaccgc gcttgaaagg cttgaaccac
     6121 agagcacaca gagaacacag agaaagagaa aat
//
LOCUS       DASQOP010000285         7539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1555877, whole genome shotgun sequence.
ACCESSION   DASQOP010000285 DASQOP010000000
VERSION     DASQOP010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7539
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555877"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /gene="rpoN"
                     /locus_tag="VE030_11525"
     CDS             <1..665
                     /gene="rpoN"
                     /locus_tag="VE030_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HZF24081.1"
                     /translation="LNPRPAASFSDGETRYVIPDVVVRKIKGLWVASLNPEAMPKLRV
                     NRMYADILHRNRDASFQQLAGQLQEAKWLIKNVQQRFDTILRVSQAIVDRQRHFFEHG
                     EVAMRPLVLREIADTLGLHESTVSRVTTQKFMLTARGIFELKYFFGSHVTTETGGACS
                     ATAIRALLKQLVGEEDRRKPLSDSQISEILGKQGIVVARRTIAKYRESLQILPVNLRK
                     SI"
     gene            692..1018
                     /gene="raiA"
                     /locus_tag="VE030_11530"
     CDS             692..1018
                     /gene="raiA"
                     /locus_tag="VE030_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HZF24082.1"
                     /translation="MNLQLSGHHLDITPAIRSYVTDKLSRVTRHFDHVIDVNVILSVN
                     KLDQKIEAKVHVRGKDIYVECVDANMYAAIDGLVDKLDRQILKHKEKSVDHHQDEAIR
                     NRPIAP"
     gene            1048..1533
                     /gene="ptsN"
                     /locus_tag="VE030_11535"
     CDS             1048..1533
                     /gene="ptsN"
                     /locus_tag="VE030_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015877040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HZF24083.1"
                     /translation="MSLLSKLLPVANVILDLDVTSKKRVFEHVGLLFENALGVGRGQV
                     FDALFARERLGSTGLGHGIAIPHGRIKGLREAAGAFVRTKAPIPFEAPDGNPVQLIFV
                     LLVPEKATDLHLQILSELAQMFSDKEMRAQLTALPDALDVQRLITEWQPHAPGQRRAA
                     V"
     gene            1502..2443
                     /gene="hprK"
                     /locus_tag="VE030_11540"
     CDS             1502..2443
                     /gene="hprK"
                     /locus_tag="VE030_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HZF24084.1"
                     /translation="MPQVSVAQLFEENADALKLAWLSGPRDPSPFLDSSEINHASQGL
                     IGHLNLVHPNWIQIFSRTEAAYFSSLSEQSQREALSRLAVSGLACIILSDQEKAPLTL
                     AEFATSRNVPLLASPLHSLQIIWVVRTYLGRALAEFVTRHGVFLDVLGMGVLITGESG
                     VGKSELALELISRGSGLVADDVVELYHIAPQTLEGRSPELLKDFLEVRGLGMLNIRTI
                     FGETAVRIRKNLKLIVQLEKPVGGVIPGLERLPLNASSEDIMGISVRKVVLPVAAGRN
                     LAVLAEAAVRNYVLQLRGIDSTQEFIQRQEKQLSKRG"
     gene            2451..3290
                     /gene="rapZ"
                     /locus_tag="VE030_11545"
     CDS             2451..3290
                     /gene="rapZ"
                     /locus_tag="VE030_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HZF24085.1"
                     /translation="MRLIVISGLSGSGKSIALNVLEDSGYYCVDNLPAKLLIGLTQFL
                     AQEDQQRVAVSIDVRSGTTLSELPDYLLQLRQRGIDLHLVFLDAKTDTLVKRFSETRR
                     RHPLAHEERTLPECIERERELLADIGDLGHRIDTSDLNPNALRAWVKDLLDLGESGLT
                     LLFQSFGFKHGIPLDADMVFDVRCLPNPHYDPELRPLTGKDAPVIAFLEADANARSML
                     ADIRDYIEKWLPCFVRDNRSYLTVAIGCTGGRHRSVYFAENLAAHFRASRGVLVRHRE
                     LST"
     gene            3376..3999
                     /locus_tag="VE030_11550"
     CDS             3376..3999
                     /locus_tag="VE030_11550"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF004685.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HZF24086.1"
                     /translation="MSLRTIALAFTGASGMPYGVRLLECLLKADLRVYLLYSQVAQVV
                     ARQEMDLALPARAKEAAEFFAARFAARPGQLQAFGREEWFAPVASGSNPPDAMVVCPC
                     TMGTLAAIAAGLADNLIERAADVTLKEARKLILVPRETPFSAIHLENMLKLARAGAVI
                     LPANPGFYHHPQTPQDLVDFVVARVLDHLSVPHSLMPRWGESGDGEG"
     gene            4255..4833
                     /locus_tag="VE030_11555"
     CDS             4255..4833
                     /locus_tag="VE030_11555"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HZF24087.1"
                     /translation="MTTSIPIFPLSTVLYPGGMLPLRIFEQRYLEMTRDCLRDATPFG
                     VCRIREGVEVGTPALPEPVGCTAVIVEWEMPHLGVFHLKARGRQPFRIRNQTTQSSGL
                     IRAEIDMLEEVPGSAPEESLVLCRRVLEQIVSRLGTEYFFPPLAYDDPRWVSYRLAEV
                     LPLALEEKQALLEIRDDRERLMRLYGLLKSSA"
     gene            complement(4840..5883)
                     /gene="mutY"
                     /locus_tag="VE030_11560"
     CDS             complement(4840..5883)
                     /gene="mutY"
                     /locus_tag="VE030_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HZF24088.1"
                     /translation="MSQFAERVIAWQRQHGRHDLPWQGTRDPYPVWLSEIMLQQTQVA
                     AVIPYFQRFLTRFPDVASLASADQDDVLALWSGLGYYARARNLHRAARIVVEQHGGVF
                     PEHFETIAALPGIGRSTAAAIAVFVSSARQPILDGNVKRVLARCFGISGFPGGTKIEK
                     RLWELAAGLLPERDVEAYTQGLMDLGAGICIRRRPRCEACPLGDECAARRENRIAELP
                     SPRPRKPMPEKSTVMLILQRNSEVLLEKRPEPGIWGGLWSFPEVSDAQEAATLSRGRF
                     GAAVKADGALPVIRHSFTHFALKITPVLLRVTRIEPRAQSPGHVWLTVEDAIGAAVPA
                     PVRTILRQLRDGG"
     gene            complement(5898..>7539)
                     /locus_tag="VE030_11565"
     CDS             complement(5898..>7539)
                     /locus_tag="VE030_11565"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HZF24089.1"
                     /translation="KLGVQLGKTRLSEFRSDQEFAGLDQMRVSLALLPLLSKQVVVDQ
                     VQLNGLRVNLVKHKDGQTNFADLLGVEEVPTPVPAVTQPSAAAAPIKFDIDGVKVRKA
                     AVSWKDEAAGTEYTVSDFNLETGRVAPGVPVKFELAAAFTASVPKMEVKFQCAGNVTA
                     DLKKQTLSADLTARIDESNVTAKVDMTNFAAPFTRFDIAFDKLDVDRYLSPTEKRATS
                     VKPASARQAEQPINFSPIKKLSLAGSLRIDHLVASNVKAQNVQMDVEARDGRLEVNSL
                     SADLYQGSTKGTASVNANNNQLAIKQSLSGVSIGPLLRDALNQDLLDGRGNVALDVTT
                     TGNTVSAIKKSLNGTVALSLKDGALKGINLAQSLRNAKAMFTGGTRDSEQAATAGEKT
                     DFSELSASFLVREGVAHNDDLLAKSPFLRLTGAGDINIAKGSLNYLAKATAVATSTGQ
                     GGKDLADTRGLTLPVRASGPFTALKYKLEFGSAFSDSAKQQVEAKKEELKGKLKDQIK
                     SRLQEATKPAGEAAPATGGGSPPSRPEDKLKEKLKKLF"
BASE COUNT         1370 a         2412 c         2281 g         1476 t
ORIGIN      
        1 gcctcaaccc acggccggcc gcgagctttt ccgacggcga gacgcgctac gtcattcccg
       61 atgtggtggt gcgcaagatc aagggcctgt gggtcgcgtc gctcaatccc gaggccatgc
      121 cgaagctgcg cgtcaaccgc atgtacgccg acattctgca ccgcaaccgc gacgcgagct
      181 tccagcagct cgccggccag ttgcaggagg ccaaatggct catcaagaac gttcagcagc
      241 gctttgacac catcctgcgc gtttcccagg cgatcgtgga ccggcagcgc cattttttcg
      301 aacacggcga agtggcgatg cgcccgctgg ttctgcgcga gatcgccgac acgctggggc
      361 ttcacgagtc caccgtatca cgcgtgacca cgcagaagtt catgctgacc gcgcgcggca
      421 tctttgaact caagtacttc ttcggcagcc acgtaaccac cgaaacgggc ggcgcctgct
      481 ccgcgaccgc catccgtgcg ctgctcaagc agctggtcgg cgaggaagac cggcgcaaac
      541 cgctgagcga cagtcagata tccgagatcc tcggcaaaca gggaatcgtg gtggcgcgcc
      601 gcaccatcgc caaataccgt gaatctttgc agatcctgcc tgtcaatctt cgcaaaagca
      661 tctgatcaac caaagcatga agggtaagcc aatgaatctt caattgtccg gtcaccacct
      721 cgacatcacc cccgccatcc gctcctacgt caccgacaaa ctttcccgag taacccgaca
      781 tttcgatcac gtcatcgacg tcaacgtcat cctgtcagta aacaagctcg accagaaaat
      841 cgaggccaaa gtgcacgtgc gcggcaagga catctacgtc gagtgcgtgg acgccaacat
      901 gtatgccgcc attgatgggc tggtagacaa gctcgatcga cagatcctca agcacaagga
      961 aaaatccgtc gatcatcatc aggatgaggc tatccgcaac agaccgattg caccataacc
     1021 tctcatcgcg tttccaggac gcccggcatg agcctgctat cgaagctgtt acctgttgca
     1081 aacgtcattc tcgatctgga tgtcacgagc aagaaacggg tgttcgagca cgtcggactg
     1141 ttgttcgaaa atgcgcttgg cgtcggccgc ggccaggtgt tcgacgcgct gttcgcgcgc
     1201 gaacggctgg gctctaccgg tctgggccat ggcattgcca ttccgcatgg ccgcatcaag
     1261 ggtttgcgag aagcggcggg cgcgttcgtg cgcaccaagg caccgatacc tttcgaggcc
     1321 ccggacggca atccggtgca actgatcttc gtgctgctgg ttcccgagaa agcgaccgat
     1381 ctgcacctgc agatactctc ggagctggcg cagatgttca gtgacaagga aatgcgtgcg
     1441 cagttgacgg cattgccgga tgcgctggac gtacaacgac tcatcacaga atggcagccc
     1501 catgccccag gtcagcgtcg cgcagctgtt tgaggaaaat gccgacgcgc tcaagctcgc
     1561 ctggctatcc gggccgcgcg atccctcgcc ctttctcgac agcagcgaga tcaatcacgc
     1621 ctcgcagggc ctgatcgggc acctgaatct ggttcatccc aactggattc agatcttcag
     1681 ccgcaccgaa gccgcgtact tttcctcgct cagcgaacag tcccagcggg aggcgctgtc
     1741 ccggctcgcc gtaagcgggc tggcctgcat catcctcagc gaccaggaga aggcgccgct
     1801 gacgctcgcc gagttcgcca cctcccgcaa tgtcccgttg ctcgcttcgc ccctgcacag
     1861 cctgcagatc atctgggtgg tgcgcaccta tctgggacgg gcgctggcag aattcgtcac
     1921 ccgccatggc gttttcctgg acgtgctcgg catgggagta ctgatcaccg gcgaaagcgg
     1981 cgtaggaaaa agcgaactgg cgctggaact catttcccgc ggcagcggcc tggttgccga
     2041 cgacgtggtc gagttgtatc acatcgcgcc gcaaacgctc gaggggcgca gtcccgaact
     2101 gctgaaggat ttcctcgaag taagaggctt ggggatgctc aacatccgca ccattttcgg
     2161 cgagaccgcg gtgcgcatca gaaagaacct gaaattgatc gtgcagctgg aaaaaccggt
     2221 tggcggcgtg attcccgggc tggaacgcct gccgctcaat gccagctccg aagacatcat
     2281 gggcatcagc gtccgcaagg tcgtgttgcc ggtcgcggcc ggccgcaacc tcgccgtgct
     2341 ggcggaagcg gcggtccgca attatgtcct gcaactgcgc ggaatcgaca gcactcagga
     2401 gttcatacag cgtcaggaaa aacagttgag caagcggggc tagagcgtcc atgcggctca
     2461 tcgtcatcag cggcctgtcc ggatccggaa agagcatcgc gctgaacgtt ctggaagaca
     2521 gcggctacta ctgcgtggac aacctgccgg cgaagctgtt gatcgggctc acgcagttcc
     2581 tcgcccagga ggaccagcag cgtgtggccg tcagcatcga cgtgcgcagc ggtacgacgc
     2641 tgtcggaact gcccgattat ctgctgcaac tccgccagcg cggcatcgac ctgcacctgg
     2701 ttttcctcga tgccaagacc gacaccctgg tcaagcgctt ctccgaaacc cgccgccggc
     2761 atccgctcgc gcacgaagag cgcacgctgc cggagtgcat cgagcgcgag cgcgaactgc
     2821 tcgccgacat cggcgaccta ggccatcgca tcgacaccag cgacctcaat cccaacgcgt
     2881 tgagggcctg ggtgaaagat ctactggact tgggtgagtc ggggctgacg cttctgttcc
     2941 agtctttcgg cttcaagcat ggcatccccc tggatgccga catggtgttc gacgtgcgct
     3001 gcctgcccaa tccccactac gacccggagc tgcgcccgct gaccggaaaa gacgcgccgg
     3061 tgatcgcgtt cctggaggcg gacgccaatg cacgcagcat gctcgcggac atccgcgact
     3121 acatcgaaaa atggctgccg tgcttcgtgc gcgacaatcg cagttatctg acagttgcga
     3181 tcggttgcac cggcggccgg caccgttcgg tctacttcgc cgagaatttg gcggcgcact
     3241 tccgcgccag ccgcggagtg ctggtccggc atagagaatt gtccacgtga gtatttccgc
     3301 atccgccgtt gcggtagcgg cgcgcagggc gcgtcgcgct gtggagccac tttcggctgc
     3361 cggcgaagcc gcgcgatgag cctgcgcacc atcgcgctcg cctttaccgg cgcttccggc
     3421 atgccctacg gggtgcgcct gcttgaatgt ctgctcaaag cggacctgcg cgtatacctc
     3481 ctttactcgc aggttgcaca ggtcgtcgcg cgccaggaga tggatcttgc tttgcccgcc
     3541 cgcgccaaag aggctgccga gttctttgcc gcacgttttg ccgcgcgtcc cgggcaactg
     3601 caggcattcg gccgcgagga gtggtttgcg ccggtcgctt caggctcgaa tccgcccgac
     3661 gcgatggtag tctgtccctg caccatgggt actctcgcgg ccatcgccgc ggggctcgcg
     3721 gacaacctga tcgaacgcgc cgccgacgtg accttgaagg aagcgcgcaa gttgatactg
     3781 gtgccgcggg aaacaccgtt ttccgcgatc catctggaga acatgctcaa gctggcgcgc
     3841 gcaggcgcgg tgatcctgcc tgcgaacccc ggcttctatc accacccgca gacaccgcag
     3901 gacctggtgg atttcgtggt cgcgcgcgtg ctcgaccatc tttccgtccc gcattcgctg
     3961 atgccccgct ggggcgaatc cggagatggc gaaggctgac ggatacaaag actcaccacg
     4021 aagagacgac gaacacgaag aggtcaaatt caagttcagg gtttccctgt gaacatctgc
     4081 attcttcggg tttcggtcac aactttcctt ccttggcttc gctgttgccg ggtggctgga
     4141 acggagcaaa aagaacatcg atcacgtcgt ttttcttcgt gtgtcttcgt gttcttcatg
     4201 ccttcgcggt gagattccgg tttgggtatt cttcgcgcac ttgacgcgat cgagatgacg
     4261 acatctatcc caatcttccc gctcagcacg gtgctctacc ccggcggcat gctgccgctc
     4321 aggatcttcg agcagcgtta tctggaaatg acccgggact gcctgcgcga cgcaacgccc
     4381 ttcggagtat gccgcatcag ggaaggcgtg gaagtcggca cgccggcgct gccggaaccc
     4441 gtcggctgca ccgctgtaat cgtcgaatgg gagatgcctc atctgggggt gtttcatctc
     4501 aaggcccggg gccggcagcc gtttcgtatc cgcaaccaga ccacgcagtc gagcggcctg
     4561 atccgcgccg agatcgacat gcttgaggag gttccgggaa gcgcgcccga ggaatcgctg
     4621 gtcctgtgcc gccgggtgct cgagcagatc gtcagccgtc tcggtaccga gtatttcttt
     4681 ccgccgctcg cgtacgacga tccgcgctgg gtgagttacc ggttggcgga agtcctgccg
     4741 cttgccctcg aggagaaaca ggcgctgctc gagatccgcg acgaccgcga acgtctgatg
     4801 cgcctgtacg ggcttctgaa gtcgtctgca taacgaacgt caaccgccgt ctcgaagctg
     4861 gcgcaggatc gtgcgcaccg gtgccggcac cgcagcaccg atcgcatctt caaccgtcaa
     4921 ccagacgtgg cccggcgact gtgcacgcgg ctcaatccgc gtcacccgca gcagcaccgg
     4981 cgtgatcttg agcgcgaagt gggtaaagct atgacgaatc accggcaagg cgccgtcggc
     5041 cttgactgcg gccccgaagc ggccgcgcga tagcgttgcc gcttcctgtg cgtcactgac
     5101 ttcggggaag ctccacaagc cgccccagat tccgggctcc gggcgctttt ccagcaagac
     5161 ttcgctgttg cgctgcagga tcagcatgac agtggatttc tccggcatgg gttttctcgg
     5221 ccgcggcgac ggcagttcgg cgatgcggtt ctcacgcctg gccgcgcact catccccgag
     5281 cgggcaggcc tcgcaacgcg ggcggcggcg aatgcagatg ccggcgccga gatccatcag
     5341 cccttgcgtg tacgcctcga catcgcgctc gggcaacaac cctgcggcga gttcccacag
     5401 gcgcttctcg attttcgtcc cgccggggaa gcctgagatg ccgaagcagc gtgccagcac
     5461 acgcttgacg ttgccgtcca ggatcggctg gcgggcgctg gagacgaaca ccgcaatcgc
     5521 agccgcggtc gatcgtccga tccccggtag cgcggcaatc gtctcgaaat gctccgggaa
     5581 tacgccacca tgctgttcga cgacgatgcg cgcggcgcgg tggagattgc gggcacgagc
     5641 gtaataaccg agcccgctcc atagcgcaag cacgtcgtcc tggtcggcgg acgccaggct
     5701 ggcgacgtcg gggaagcggg taaggaagcg ctggaaatac gggatgaccg cggcgacctg
     5761 tgtctgctgc agcatgattt cggagagcca gaccggatag ggatcgcgcg tgccctgcca
     5821 cggcagatcg tggcgtccat gctggcgttg ccaggcaatg acgcgctcgg cgaattggct
     5881 catcccggaa tcgtcgctca gaacagcttc ttcagctttt ccttgagttt gtcttccggc
     5941 cgcgacggcg gcgatccccc gcccgtggcg ggcgctgcct ccccagcggg cttcgtcgcc
     6001 tcctgcagcc tggacttgat ctggtctttg agcttgccct tgagttcctc ctttttggcc
     6061 tcgacctgct gcttagcgct gtccgagaac gcggaaccga actcgagttt gtatttgagc
     6121 gcagtgaacg gacccgaagc gcgcaccggg agcgtcagcc cacgggtgtc ggcgaggtcc
     6181 tttccgccct ggccggtgct cgtcgccact gccgtagcct tcgccaggta gttcaggctg
     6241 cctttcgcga tgttgatgtc ccccgcgccc gtcagccgca ggaacgggga cttcgccaac
     6301 aaatcgtcgt tatgggccac gccctcgcgg actaggaaac tggcgctcaa ctcggagaaa
     6361 tcggtctttt cgccggcggt tgcggcctgc tcggaatcgc gcgtcccgcc ggtgaacatc
     6421 gctttggcgt tgcgcagcga ctgcgccagg ttgatgccct tgagtgcgcc gtccttgagg
     6481 ctgagcgcca cggtgccatt gagcgacttc ttgatcgcgc tcacagtatt gccggtggtg
     6541 gtgacgtcga gcgcgacgtt gccgcgccca tcgagcaggt cctggttcaa cgcatcgcgc
     6601 agcaacgggc cgatcgatac gccggaaagg ctttgtttga tcgcgagctg gttgttgttg
     6661 gcgttgaccg aggccgtccc cttggtgctg ccctgataga ggtcggccga tagcgaattc
     6721 acctccagcc tgccgtccct ggcctcgacg tccatttgca cgttctgcgc cttcacgttc
     6781 gacgcgacca ggtggtcgat gcgcaggctg ccggccaggc tgagtttctt gatcggcgaa
     6841 aaattgatcg gttgctcggc ctgccgggcc gatgccggtt tgacgctcgt cgcccttttc
     6901 tccgtgggtg acaggtagcg gtccacgtcg agtttgtcga aggcgatgtc gaagcgggta
     6961 aatggcgccg cgaagttggt catgtcgact ttggccgtga cgttgctttc gtcgatcctg
     7021 gcggtcagat cagcgctcag cgtctgtttc ttcaggtcag cggtcacgtt tccggcgcac
     7081 tgaaacttca cctccatctt cggtaccgat gccgtgaatg ccgcagcaag ctcgaatttg
     7141 actggtaccc cgggagccac ccgcccggtc tcaaggttga aatcggacac cgtgtactcg
     7201 gtgccggccg cctcatcctt ccagctcact gcggccttcc tcacctttac gccatcgata
     7261 tcgaacttga tcggggctgc ggccgcggac ggctgcgtca ccgcgggcac aggtgtcggg
     7321 acttcttcca ctccaagcag atcggcaaaa ttggtctgtc cgtccttgtg tttgacgagg
     7381 ttaacccgca atccgttgag ctggacctgg tccaccacca cctgtttcga cagcagcggg
     7441 agcaaagcca gcgacacccg catctgatcc aatccagcaa actcctggtc gctcctgaac
     7501 tcggacaggc gtgttttccc cagttgtacg ccaagcttc
//
LOCUS       DASQOP010000286         2324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1353265, whole genome shotgun sequence.
ACCESSION   DASQOP010000286 DASQOP010000000
VERSION     DASQOP010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353265"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..1355
                     /locus_tag="VE030_11570"
     CDS             126..1355
                     /locus_tag="VE030_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZF24090.1"
                     /translation="MALFAYKGRNASGELVQGVAEGADSAAVATQLFATGVTPVEIGE
                     TQRRQAGESIGALQRLTQRPVAHEDLLLFSRQMYTLLKAGVPIMRSLAGLQESTGNPT
                     FARVLQNIRENLDSGHPLSVALQRQGRVFSPFYVAMVYVGETTGMLEEIFLRLFNHLE
                     FQEFMRGQVKSALRYPSFVLVAMAVAIAVINLFVIPAFAKVYKGFNAELPFITKLLIG
                     FSDLMVSSWPFLLAALVGAALAFRGYTRTKAGKFAWDRFKLRIPIAGKIILKATLARF
                     SRSFALSMRSGVPVVQGLTLVAQTVDNSFVAERVEKMREGVERGDSVLHSAVNAGVFT
                     PVVLQMLMVGEESGALDDMMQEIADMYQREVEYELKTLGAQIEPILILCLGVLVLILA
                     LGVFLPIWDLGQAALKK"
     gene            1359..1886
                     /locus_tag="VE030_11575"
     CDS             1359..1886
                     /locus_tag="VE030_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24091.1"
                     /translation="MRIFPVRGGQCGASFVELAVCMALMGIFVGVLLERSLYYQEYAE
                     KTAMESTAQNIRTGLRYKVADLILANRVSEIQTLADENPMNWLAERPPNYLGELDSAP
                     TDEPKGQWYFDKRDRELVYTVDNRRHFSPSMYRDFTVRYRAMRVSTGTVTESSPGSAD
                     TWVSLVLLGEYDWFK"
     gene            1967..>2324
                     /locus_tag="VE030_11580"
     CDS             1967..>2324
                     /locus_tag="VE030_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HZF24092.1"
                     /translation="MKSFEFAIIRRRSGGFTLIELVVTISIIAILAAVALPRYITLQT
                     QARAAKTQAIFGGVRSAAALAHAQAIATNSTAAATVSMEGVLVDQVNGYPAATNTGIV
                     TALQLDPNGDQVTITQG"
BASE COUNT          435 a          654 c          767 g          468 t
ORIGIN      
        1 taccaccgtc gacgaagcga tgcgcgtcag tgcgctactg gaagaaggac ttgatttact
       61 accaccgagt tgatcaacat gcagatcgag ggaattcccg tgttcgccgt ggtaagtacc
      121 tagccgtggc tcttttcgcc tacaagggca gaaacgcctc cggcgagctg gtgcagggcg
      181 tggccgaggg cgccgacagc gccgcggtcg cgacccagct gttcgccacc ggggtcacgc
      241 cggtggagat cggcgaaacg cagcgccggc aggcgggcga atcgatcggt gcattgcagc
      301 ggctgacgca gcgcccggtc gcgcatgagg acttgctgct gttcagccgg cagatgtaca
      361 cgctgctcaa ggccggcgtg ccgatcatgc gctcgctcgc gggactgcag gagtccactg
      421 gcaatccgac tttcgcgcgg gtgctgcaga acattcgcga gaacctggac agcggccatc
      481 cgctgtcggt ggcgctgcag cggcagggcc gcgtgttttc gcccttctac gtggccatgg
      541 tgtacgtcgg cgaaaccacc ggcatgctcg aggaaatctt tctgaggttg ttcaatcacc
      601 tcgagttcca ggaattcatg cgcggccagg tcaagtcggc gttgcgctat ccgtcgttcg
      661 tgctggtcgc gatggcggtc gcgatcgccg tcatcaacct tttcgtcatc ccggcgtttg
      721 ccaaggtgta caagggcttc aatgccgaac tgccgttcat cacaaaattg ctgatcgggt
      781 tttccgactt aatggtttcg agctggccgt tcctgctggc cgcgctggtc ggggcggcgc
      841 tcgcgtttcg cggctacacc cgcaccaagg cgggaaagtt cgcgtgggat cgtttcaagc
      901 tgcgcatccc gatcgcgggc aagatcatcc tcaaggcgac gctggcgcgt ttctcgcgca
      961 gcttcgcgct gtcgatgcgc agcggggtcc cggtggtgca gggactcaca ctggtggcgc
     1021 agaccgtgga caacagcttc gtcgcggaaa gagtcgaaaa aatgcgtgaa ggcgtcgagc
     1081 gcggtgacag cgtgctgcac tccgcggtca acgccggggt tttcactccg gtggtattgc
     1141 agatgttgat ggtgggcgaa gagtccgggg cgctcgacga catgatgcag gaaatcgccg
     1201 atatgtatca gcgcgaggtc gagtacgaac tgaaaacgct gggcgcgcag atcgagccga
     1261 ttttgatcct gtgcctcggc gtactggtgc tgatccttgc gctgggcgtg ttcctgccga
     1321 tctgggacct tggccaggcg gcactgaaga aatgagcgat gcgtatattt cccgtgcgcg
     1381 gcggccagtg cggcgcgagt ttcgtggagt tggccgtgtg catggcgctc atgggcatct
     1441 tcgtgggggt gctgctcgag cggtcgctgt actatcagga gtacgcggaa aaaaccgcga
     1501 tggaatcgac ggcacagaac atccgcaccg gtttgcgcta caaggtggcg gacctgattc
     1561 tcgccaaccg ggtgtcggaa attcagacgc tggccgacga gaatccgatg aactggcttg
     1621 cggaaaggcc cccaaattat ctgggcgaac tggattccgc gcccaccgac gaaccgaagg
     1681 gccagtggta tttcgacaag cgggaccggg agctggtgta cacggtggac aatcggcggc
     1741 acttttcgcc gtcaatgtat cgggatttca ctgtccgcta ccgtgcgatg cgggtgagta
     1801 cgggaacagt tacggagagc tcgcccggtt ctgcagacac atgggtgagt ttggtgctgc
     1861 tcggcgagta tgattggttc aagtagtaca ggcatggatc gtgctttgtt tttgaagatc
     1921 gttttttgcg tgcgcgtgtt cgtcgatacg agggagttaa atagtgatga agtcatttga
     1981 attcgcaata atcaggcgcc gcagcggcgg ctttaccctg atcgagctgg tggtcacgat
     2041 cagcatcatc gccattctgg cggcggtcgc gctgccgcgt tacatcacac tgcaaacgca
     2101 ggcccgggcc gcgaagacgc aggcgatatt cggtggcgtg cgctcggcgg ccgctctcgc
     2161 gcacgcgcaa gcgatcgcca ccaacagcac agcagcggcg acggtctcga tggagggcgt
     2221 attggtcgat caagtcaacg gctacccggc cgcgaccaac accggtattg tcaccgcgct
     2281 gcaactggac ccaaacggcg accaagtgac catcacgcag ggcc
//
LOCUS       DASQOP010000287         4735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1353594, whole genome shotgun sequence.
ACCESSION   DASQOP010000287 DASQOP010000000
VERSION     DASQOP010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353594"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..1594
                     /gene="amrB"
                     /locus_tag="VE030_11585"
     CDS             218..1594
                     /gene="amrB"
                     /locus_tag="VE030_11585"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HZF24093.1"
                     /translation="MSSVRAPAVAGAFYPATTAPLQAQIAELLAAARPPSLQRPKALI
                     APHAGYVYSGPVAANAYALLAPFRSDYSRVVLLGPAHRVYVRGLALPAADAFATPLGE
                     VGLDREAIAAIRHLPQVCTSDDAHAHEHSLEVHLPFLQQALAGFTLAPLAVGEASAEQ
                     VAEVLGLLWGDQRTLIVISSDLSHYLPYAQARTIDEQTARMILNMAPQLNHQQACGAT
                     PVNGLLLEARGRGLRPELLDLRNSGDTAGDKSRVVGYASFAFYDQASTREKQDDKQEC
                     ADGRVLVSIARSSIAGSFGIPTSVQEDAAFLHHPAASFVTLKRENRLRGCIGSLRARR
                     TLVEDVKANARAAAFHDPRFAPLSFDELSTTKIEVSVLSALAPLAFRDQEHALAQLRP
                     GEDGVVLEFGRHRGTFLPQVWENLPSSASFLDELKRKAGLPRDFWDDGIRLFRYSVSR
                     WAEPEECL"
     gene            1585..2679
                     /gene="amrS"
                     /locus_tag="VE030_11590"
     CDS             1585..2679
                     /gene="amrS"
                     /locus_tag="VE030_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HZF24094.1"
                     /translation="MPLNAPEQRPYPGRWWHMLDDGRLQCDLCPRDCRLHEGQRGACF
                     VRQRINDSMVLTTYGRSSGFCIDPIEKKPLNHFYPGSAVLSFGTAGCNLACKFCQNWD
                     ISKSRDMDRLMDQAGAEDIAAAAERSGSRSVAFTYNDPVIFAEYAMDVADACHARGIQ
                     TVAVTAGYMHDQPRREFYAKMDAANVDLKGFTDDFYVRLTGSKLQPVLDTLKYLRHET
                     DVWFEITTLLIPTKNDSDEEIYQLSKWVAKELGSDVPLHFTAFHPDYKLDDLPPTPPA
                     TLTRARRIALGEGLNYVYTGNVHDVEGGTTFCTGCSTALIVRDWHRILTYRLDPQGKC
                     PSCGTAAAGRFEKFTRAFGPRRIPISIQVA"
     gene            complement(2898..3518)
                     /locus_tag="VE030_11595"
     CDS             complement(2898..3518)
                     /locus_tag="VE030_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HZF24095.1"
                     /translation="MFADRHEAAIRLAAEVGKYRGLNPLVLGIPRGAVPMARVIAAEL
                     AGEVDVTLVRKLGAPGNPEFAVGAVDETGWSHVAAHARGAGADAAYLEQETRAQVEVL
                     KARRAAYTAIRPAVDPRGRIVIVVDDGLATGATMIAALHALRARNPAKLICAVPVAPP
                     ESIEKVSRHCDEMMCLEAPGNFRSVGQFYRRFEQVSDAEVAAALKA"
     gene            complement(3530..4429)
                     /gene="folD"
                     /locus_tag="VE030_11600"
     CDS             complement(3530..4429)
                     /gene="folD"
                     /locus_tag="VE030_11600"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HZF24096.1"
                     /translation="MTAALIDGVAVSKAIRAQWQQRVGALKARGVTPGLAVILVGDNP
                     ASQVYVRNKVRACGEVGLYSEVHPYPANVSQETVLAKIEELNQDPKIHGILVQLPLPP
                     HFDNSKVLEVISAEKDADGFHLYNLGGLVSGGAVFPPCTPYGVMALLDYYKIPIEGSN
                     AVMVGRSNIVGKPMALMLLARSATVSICTSKTRDLKQFTTLADILVVATGKPRMITGD
                     MIKPGSTVIDVGTNRMPDGKLVGDVDFEGARQRAAFITPVPGGVGPMTITMLIANTVQ
                     AAERFVSRQVKAHADKVNASVLP"
BASE COUNT          865 a         1545 c         1414 g          911 t
ORIGIN      
        1 agcggcgatc ctgtcgatgc tgctgattca tcatacttat gtaagtgact gagcggtcag
       61 taatatacac gcgtccgtca aatgttcaac ctcttttctc acctggttgc cagtttcagc
      121 cctcacatcg ccacattgcc agtccggcgc ctataatttt tcgtttgccc tttgccatac
      181 gggtccacaa actcgcaatc gtcttcagga cacagccatg agttcagtca gggcaccggc
      241 agttgccggc gccttctatc cggccaccac ggccccgctg caggcgcaaa tcgcggagtt
      301 gctggcggcc gcgcggccgc ccagcctaca acgacccaag gcgctcatcg cacctcacgc
      361 cggttacgtc tattcgggtc cggtcgcggc gaacgcttac gcgctgctgg cgccgtttcg
      421 cagcgactac tcccgagtgg tgctgctcgg cccggcccac cgtgtgtacg tgcgcggact
      481 ggcgctgccc gcggccgatg cctttgccac gcctctgggc gaagtgggct tggaccgtga
      541 agcgatcgcg gcgatccggc atctgccgca agtttgcacc agcgacgatg cgcacgcaca
      601 cgagcattcg ctggaggtgc acctgccctt cctgcagcag gcgctcgccg gattcacgct
      661 ggcgcccctt gcggttggcg aggcctctgc cgaacaggtt gccgaagtgc tcggcctgct
      721 ttggggcgat cagcgcacgc tgatcgtcat cagttccgac ctttcccatt accttcccta
      781 tgcgcaggcc agaacgatcg atgaacaaac cgcgcgcatg atcctgaaca tggcaccgca
      841 gctcaaccac cagcaggctt gtggtgcgac gccggtcaat ggactgttgc tggaagcgcg
      901 tggccgagga ctgcgcccgg aattgctgga cctgcgcaac tcgggcgata ccgccggcga
      961 caagagcagg gtggtgggat acgcatcgtt cgcgttttac gaccaggcgt ccacgcggga
     1021 aaagcaggac gacaaacagg aatgcgcaga cggccgggtc ctcgtttcga ttgcacgttc
     1081 ttcgattgcc ggcagcttcg gcatccccac ttcagtgcag gaagacgcag cgttcctgca
     1141 ccacccggca gcatctttcg tgaccctgaa acgcgagaac cggctgcgtg gctgtatcgg
     1201 cagcctgcgg gcgcgccgca cgctggtcga ggacgtaaag gcaaacgccc gcgccgcggc
     1261 gttccacgac ccccgctttg cgccgctttc ctttgacgaa ttgtccacca cgaaaatcga
     1321 ggtgtcggtg ctgtcggcac tcgcgccgct ggcattccgc gaccaggaac acgcactggc
     1381 gcagttgcgc cccggcgagg atggggtggt gctcgaattc gggcgccatc gcggcacctt
     1441 ccttccccag gtatgggaaa acctgccctc gtctgcctcg tttctcgacg aactcaagcg
     1501 caaggccggc ctgccgcggg acttctggga cgacggcatc cggttgttcc gctattcggt
     1561 gtccaggtgg gccgagccgg aggaatgcct ttgaacgcgc cggaacagcg gccttatccg
     1621 ggacgctggt ggcacatgct cgacgacggc cgcctgcagt gcgacctgtg cccgcgcgac
     1681 tgccggctgc acgaaggcca gcgcggcgcc tgcttcgtgc gccaacggat aaacgatagt
     1741 atggtgctca ccacctacgg ccgctcctcc ggattctgca tcgatccgat cgagaagaag
     1801 ccgctgaatc acttctatcc cggctcagcc gtactttcat tcggcaccgc gggctgcaat
     1861 ctcgcgtgca aattctgcca gaactgggac atttccaaat cccgcgacat ggatcgcctg
     1921 atggatcagg ccggcgccga ggacatcgcg gcagcggcgg aacgctccgg cagccgaagc
     1981 gttgcgttca cctacaatga cccggtgatc ttcgcggaat atgcgatgga cgttgccgac
     2041 gcctgccatg cgcgcggcat ccagaccgtg gcggtgaccg cgggctacat gcacgatcag
     2101 ccgcgcaggg agttttacgc gaagatggac gcggcgaacg tggatttgaa gggattcacc
     2161 gacgacttct acgtcaggtt gaccggttca aaactgcagc cggtgctgga cacgctcaag
     2221 tacctgcgtc acgaaaccga tgtatggttc gagatcacta cactgctgat cccgaccaag
     2281 aacgactcgg acgaggaaat ctaccaactc tcaaaatggg tggctaaaga actgggcagc
     2341 gacgtgccgc tgcacttcac cgcgttccac ccggactaca agctcgacga tctgccgccg
     2401 actccgcccg ccacgctgac ccgcgcacgc cggattgccc ttggtgaggg gctgaattac
     2461 gtctacaccg gcaatgtgca cgacgtggaa ggcggcacca ctttctgcac cggctgcagc
     2521 accgcgctga ttgtccgcga ctggcaccgc atcctgacct atcgcctcga tccgcagggc
     2581 aaatgcccgt cctgcgggac tgcggccgcg gggcgcttcg agaaattcac cagggctttc
     2641 ggcccccgcc gcatccccat ctccatccaa gttgcctaaa gcttagttcc ttaccgcaaa
     2701 ggcgcaaaga cgcaaaggaa acgcaaagaa gaacttgact gggcaaaaat ctgatcggcg
     2761 cagaagggga cattgaagcg tacggaccat tcatcaacga aaagtccaga ccttatgttc
     2821 tttcgcccat tcatgttttt cttgatttac tttgcgtttc ctttgcgtct ttgcgccttc
     2881 gcggtgaagg tcttgatcta ggctttcaga gcggcggcga cttcggcgtc ggagacctgc
     2941 tcgaagcgcc ggtagaactg tcccacggag cggaagttgc cgggtgcttc caggcacatc
     3001 atctcgtcgc agtggcgcga cactttttca attgattccg gcggcgccac cggcaccgcg
     3061 cagatcaact tggcgggatt gcgcgcgcgc aatgcatgca gcgccgcgat catggtggcg
     3121 ccggtggcaa gtccgtcgtc caccacgatg acgattcgac cccgagggtc caccgccggc
     3181 cggatcgcgg tataggcggc acgccgggcc ttcagtactt cgacctgcgc ccgcgtctcc
     3241 tgctccaggt acgctgcatc ggcgccggcg ccgcgcgcgt gcgcggccac gtgcgaccag
     3301 ccggtctcat ctactgcgcc gaccgcgaac tccgggttgc cgggcgcgcc gagcttgcgc
     3361 accagcgtca cgtcgacttc gcctgcaagt tccgcggcga tgacacgcgc catcggtacc
     3421 gcgccgcgcg ggatgcccag caccagggga ttcaggccgc gatatttccc gacttccgcg
     3481 gcaagccgga tcgctgcttc gtggcgatcg gcgaacatga caaaaagcgt tacggaagga
     3541 ccgacgcgtt gactttgtcg gcgtgcgctt tgacctgcct ggacacgaag cgctccgctg
     3601 cctgcacggt gttggcgatc agcatggtga tggtcatcgg accgacgccg cccggcaccg
     3661 gcgtgatgaa tgcggccctt tgccgcgcgc cttcgaagtc aacgtcgccg acgagtttgc
     3721 cgtcgggcat ccggttggtg ccgacgtcga tcacggtcga gcccggcttg atcatgtcgc
     3781 cggtgatcat tcgtggtttt ccggtggcca ccaccagaat atccgcgagc gtcgtgaact
     3841 gtttcagatc gcgggtcttg gaggtgcaga tgctgacggt ggcgctgcgc gccagaagca
     3901 tcagcgccat cggcttgccg acgatgttgc tgcggccgac catcaccgca ttactccctt
     3961 cgatcggaat cttgtagtaa tccagcagcg ccatcacgcc ataaggggtg cacggcggga
     4021 acacggcgcc ccccgagacc aggccgccga gattgtaaag atggaaccca tcggcgtcct
     4081 tttccgccga gatgacttcc agcaccttgc tgttgtcgaa atgcgggggc agcggcagct
     4141 gcaccaggat gccgtggatc ttcggatcct gattcagttc ttcgatcttc gccagcaccg
     4201 tctcctgcga aacgttcgcg ggatacgggt gcacctcgga atacagcccg acttcgccgc
     4261 aggccctgac cttgtttcgc acgtagacct gcgaagcggg attgtcgccg accaggatca
     4321 ccgccagccc cggggtcacg ccgcgcgcct tgagcgctcc gacgcgttgt tgccactgcg
     4381 cgcggatcgc cttcgaaact gcgacaccgt cgatgagggc cgcggtcatg cagtgaccct
     4441 ataacaggtg aagggtgaca ggtgacaggg aaagacgagt gctgcgtgct gtgtgctgag
     4501 tgctgagaaa cggcgaggac tcgggactcg ggactcagca aaaccgacgg ctgttaaaac
     4561 tatggggact gaacccgtcc tttgctcttt cctcacgcct ttcccccagc acctagcacc
     4621 cagcactcag cacttgtttt tcttccctca cgcctcgccc ttcaccttct ctatctccgc
     4681 aagtgtggag aaaaacaccg ggccgcggcc catgtccgct tcgcgctggc tgatc
//
LOCUS       DASQOP010000288         7878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2163710, whole genome shotgun sequence.
ACCESSION   DASQOP010000288 DASQOP010000000
VERSION     DASQOP010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7878
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2163710"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VE030_11605"
     CDS             <1..187
                     /locus_tag="VE030_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24097.1"
                     /translation="PDRFFLVDRVDTQYSASTGLIFSPWKSVRVTTQASGTRNDSNIP
                     INAYDRVVYQITLRYDI"
     gene            complement(258..791)
                     /locus_tag="VE030_11610"
     CDS             complement(258..791)
                     /locus_tag="VE030_11610"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HZF24098.1"
                     /translation="MAVIARTSILNPQSPRISIIVAYAVNRVIGKDGKVPWHLSGDLK
                     RFRQLTMGHHIVMGRKTWESIGRLLPGRKHIIVSRQPDYAVPGAVVTRTIEETLAACA
                     GDNEIFVIGGEEIYRLVLPIAERIYATEIMADFAGDTFFPPFDPGTWVEVSREMGTNS
                     ESLPHDFVIYERAAIRR"
     gene            complement(1123..1917)
                     /locus_tag="VE030_11615"
     CDS             complement(1123..1917)
                     /locus_tag="VE030_11615"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HZF24099.1"
                     /translation="MRQYLDLMRHILEHGARKGDRTGTGTLSVFGPQLRFDLQAGFPL
                     LTTKKVHLKSIIHELLWFLKGETNIRYLKENGVSIWDEWADDEGELGPVYGYQWRSWP
                     APDGRHIDQMAKAIEEIRRNPDSRRLIVSAWNVADLESMALMPCHAFFQFYVADGKLS
                     CQLYQRSADLFLGVPFNIASYAALTLMVAQVCNLRPGEFVHTFGDTHLYLNHLEQARE
                     QLSRQPRTLPRMILNSEIRNLFDFSYEDFNLVGYQPYPAIKAPVAV"
     gene            2077..3237
                     /gene="aroC"
                     /locus_tag="VE030_11620"
     CDS             2077..3237
                     /gene="aroC"
                     /locus_tag="VE030_11620"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004266572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HZF24100.1"
                     /translation="MSGNTLGKLFCVTSFGESHGPAIGCVVDGCPPGLELSEADIQKE
                     LDRRKPGTSRHVTQRREPDTVEILSGVFEGRTTGTPIALLIRNQDQRSKDYGNIAETF
                     RPGHADYTYWQKYGIRDYRGGGRQSARETAVRVAAAAIAKKWLWERHGVVIRGYMSQL
                     GSIRIAFRTWDAVNANPFFAPDPDIVPKLEEFMDRLRKSGDSCGAKITVVAENVPVGW
                     GEPVYDKLDAEVAYAMMGINAVKGVEIGAGFGAVEQKGTEHGDEMTPEGYLSNNAGGI
                     LGGISSGQDIVVNIAVKPTSSIRLPRRSIDKHGEPTVVETTGRHDPCVGIRATPIAEA
                     MLALVLMDHALRHRAQNADVRCPTPRIAARAPAGAASAGNPSQATDPDPDEA"
     gene            3252..3803
                     /locus_tag="VE030_11625"
     CDS             3252..3803
                     /locus_tag="VE030_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017407019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24101.1"
                     /translation="MSWLQRLAEVNEQDWLPLGTPAFVAATLGIALVAFLANTGQRWV
                     FLLDNANLALHEAGHPIFGLLLGDRITVYGGTLAQLLFPVAAAATFWRRREALSFALC
                     AVWLFENFWNIARYMADARAQVLPLVGSGEHDWTEIFSRWGVLGRDTGIAGFVTLLGW
                     LGVLIAWGWLARRWWLERELGQR"
     gene            3821..4981
                     /locus_tag="VE030_11630"
     CDS             3821..4981
                     /locus_tag="VE030_11630"
                     /inference="COORDINATES: protein motif:HMM:NF037955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZF24102.1"
                     /translation="MARGSFYFRLSNFYFWYFAFIGAFAPYFALYLKSLGFSASQIGV
                     LLAVNPVARVLGPNIWGWLSDHYGARGQLIRLTAVGTAVAFVAVFFNRGIGWMFTALL
                     VLNIFWCAVLPLAESATLNLLGSRVGTYGRIRLWGSVGFVAVVVAGGYCLDYFGINSL
                     APMVLTLLVLMAASTFPLPRDREPSHHGEHAPILRIVTQPAVIALFAGFFLMQVAHGP
                     YNGFYSIHLVEAGYSKKAVGWLWALGVMAEIGLFMWLPRLMRAYSLNQILLFSFGCAF
                     ARFLLIAWGVGSIPVMVVAQLMHAITFGAYHAAGVAAMHQIFRGRNQARGQALYTSLG
                     YGLGGTLGTLGAGYAWDGWGPQWTFTFAAVAALLAFLVVSWKPMSFGTKREI"
     gene            5045..6454
                     /gene="leuC"
                     /locus_tag="VE030_11635"
     CDS             5045..6454
                     /gene="leuC"
                     /locus_tag="VE030_11635"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HZF24103.1"
                     /translation="MNPQTLYDKLWASHLVRQEPDGTALIYIDRHLVHEVTSPQAFEG
                     LKLAGRKPWRIDSVVATADHNTPTTDWDKGIRDPISRLQVETLDANIREFGAKAYFPF
                     LDRRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFGCLAHGIGTSEVEHVLATQCL
                     MQKKMKNMLIEVNGALGRGVTAKDVALAVIGKIGTAGGSGHAVEFGGSAVRALSMEAR
                     MTLCNMAIEAGARAGMVAVDETTIEYLRGRAFAPIGKMWDRAVTHWRTLKSDDNARFD
                     FVVRLDAREIKPQVTWGTSPEMVVPVDGRVPDPDKEKDAVRREGMERALTYMGLKPNT
                     QITDIRVDKVFIGSCTNSRIEDLRAAAQIVRGRRVAANVRLAMVVPGSGLVKEQAEKE
                     GLDRVFRDAGFEWRQPGCSMCLAMNDDRLEPGERCASTSNRNFEGRQGAGGRTHLVSP
                     AMAAGAAIAGHFVDVRSGL"
     gene            6476..7117
                     /gene="leuD"
                     /locus_tag="VE030_11640"
     CDS             6476..7117
                     /gene="leuD"
                     /locus_tag="VE030_11640"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HZF24104.1"
                     /translation="MEKFTRLEGLAAPLDRANVDTDAIIPKQFLKSIRRSGFGVNLFD
                     AWRYLDHGEPGMDPVTRKPNPDFVLNQPRYRGANILLVRKNFGCGSSREHAPWALGDF
                     GFRALIAPSYADIFYNNCFKNGLLPVVLPESQVDRLFHDIAAFPGFRLVIDLGAQTVA
                     YPDGSQVFTFEIDSFRRHSLLNGLDDIGLTLRHVDEIKAFEAKRRVEQPWLFS"
     gene            7214..>7878
                     /locus_tag="VE030_11645"
     CDS             7214..>7878
                     /locus_tag="VE030_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HZF24105.1"
                     /translation="MKIAVLPGDGIGPEIVKQALKVLRALSREGQKFDLQEAPIGGAG
                     YDAGGDPLPESTLALAKSADAVLLGAVGGPKYDKLPRPMRPEQGLLRIRKELGLFANL
                     RPALLYEELANASTLKSDVVAGLDIMILRELTGDIYFGQPRGRRETGSGEREGYDTMR
                     YGESEIRRIAHVGFGIARKRAKRLCSVDKANVLDTSILWREVVNEVAEEYPDVELSHM
                     YVD"
BASE COUNT         1426 a         2358 c         2563 g         1531 t
ORIGIN      
        1 ccccgatcgg tttttcctgg tcgatcgcgt agacacccag tacagcgctt ccaccggttt
       61 gatcttctcc ccgtggaaaa gtgtccgggt gaccacccag gccagcggga cccggaacga
      121 ctcgaatatt ccgatcaacg cgtacgaccg ggtggtctac cagatcaccc tgcgctatga
      181 catataggcg caacgaaggc gattggcggc gacaacccta aagtatccgg ggaccggcga
      241 gctacggcga ggcaccatta acggcggatc gcagcccgct cgtagatcac gaagtcgtgc
      301 ggaagcgatt cggagttcgt gcccatctcc cgcgaaacct ctacccatgt accgggatcg
      361 aacggaggga agaaagtgtc gcctgcgaaa tcggccatga tctcggtagc gtagatgcgc
      421 tcggcaatgg gcagcaccag acgatagatc tcttcgccac cgatcacgaa tatttcgttg
      481 tcgccagcgc atgccgcaag cgtttcctcg atggtgcgtg taacgactgc gccaggcacc
      541 gcgtagtctg gctgccggct gacgatgatg tgcttgcgtc ccggcagcag cctgccgatc
      601 gactcccagg tcttgcggcc catcacgatg tgatgtccca tcgtgagctg gcggaagcgc
      661 ttcaggtctc cggaaagatg ccagggcacc ttcccatcct tgccgatgac gcgattcacc
      721 gcgtaagcaa cgatgattga gattcgaggg gattggggat tcaggattga ggttcgagct
      781 attacagcaa ttgctgataa gtccgcttac ttgtttagtg agttcttgag ggtgctaagc
      841 attttaccga catgatctga cttggcgaga aggcgtgtca cgtcaacttc cgtagcgtga
      901 tcgagtctca aggcaatctg gagttgtgtt tctagttcca tcaatgatcc atcaggggga
      961 aggatctcga caatcgatag acctcggtta ccaaacccat ggcttcttgc cagactcgga
     1021 ggtcgctgta cctcattcag cattgcctac aagtgccgca atgccgaccc gcggctggtt
     1081 ccctcacttc ccatcctcaa tcccgaatcc ccaatcccgc tctcacaccg ccaccggcgc
     1141 tttgatggcg ggatagggtt ggtagccgac cagattgaaa tcttcgtagc tgaaatcgaa
     1201 caggttcctg atttcggagt tgaggatcat gcgcggcaac gtgcgcggct gccgcgaaag
     1261 ctgctcacgg gcctgttcca ggtggttgag gtacaggtgg gtatcgccga aggtatgcac
     1321 gaattcaccg ggcctcaggt tgcacacttg tgccaccatc agcgtcagag ccgcgtaaga
     1381 ggcgatgttg aaaggcacgc cgaggaaaag gtcggcgctg cgctggtaga gctggcagga
     1441 gagtttgccg tcggcaacat agaactggaa gaaagcgtga cagggcatga gcgccatgct
     1501 ttcgagatcg gcgacgttcc aggccgacac gatgaggcgt cgagagtccg ggttgcgccg
     1561 gatttcctcg atcgccttcg ccatctggtc gatatggcgc ccgtccggcg ccggccagga
     1621 acgccactga tagccgtaca ccggaccgag ttcgccctca tcgtcggccc attcgtccca
     1681 gatcgacacc ccgttttcct tgagatagcg gatgttggtt tcgcccttca ggaaccacag
     1741 cagttcgtgg atgatggact tgaggtggac tttcttggtg gtgagcagag gaaatccggc
     1801 ctgaaggtca aagcgcaact gggggccgaa tacactcagc gtccccgtgc ccgtgcgatc
     1861 gcctttgcgg gcgccgtgtt cgaggatatg tcgcatcagg tcgaggtact ggcgcatggc
     1921 cctgggctgc tcggcatggg gaagatgagc aggtattcta aatcaggccg cccggcggcg
     1981 catcgggccg atgattccgc attcggcgca agccgcattg ctgcagggtt tctgcggggc
     2041 gcgtgataaa cttcgacgct tctcttcggg tgcgcaatgt ccggaaacac gctcggcaaa
     2101 ttgttctgcg ttacgtcctt tggcgagagc catggcccgg caatcggctg cgtcgtggac
     2161 ggttgtccgc cgggattgga gctctccgaa gccgacatcc agaaagaact cgatcgccgc
     2221 aagcccggta catccaggca tgtcacgcag cggcgcgagc ccgatacggt ggagattctt
     2281 tccggcgtgt tcgaaggccg gaccaccggc acaccgatcg cgctgctgat ccgcaaccag
     2341 gaccagcgca gcaaggatta cggcaacatc gccgaaactt tccgtcctgg tcacgcggat
     2401 tacacctatt ggcaaaagta cggcatccgc gactaccgcg gcggcggacg gcagtcggct
     2461 cgcgagacgg cggtgcgtgt ggcggcggcg gcgattgcaa agaagtggct gtgggagcgc
     2521 cacggtgtgg tgattcgcgg gtacatgtcg caactcggca gcatacgcat tgcattcagg
     2581 acctgggacg cggtgaacgc caacccgttc tttgcgcccg atccggacat cgtgccgaaa
     2641 ctcgaggagt tcatggaccg gctgcgcaag tccggcgact cgtgcggggc gaaaatcacc
     2701 gtggtcgcgg aaaacgtgcc ggtaggctgg ggcgagcctg tttacgacaa gctcgatgcc
     2761 gaagtcgcct atgccatgat gggaatcaac gcggtaaaag gtgtggaaat cggcgccggc
     2821 ttcggcgcgg tcgagcagaa gggcaccgag cacggcgatg aaatgacgcc ggaaggctat
     2881 ctgagcaaca atgccggcgg catccttggc ggcatctcca gcggccagga catcgtggtc
     2941 aatattgcgg taaagccgac ctcgagcatc cgcctgccgc ggcgttcgat cgacaagcac
     3001 ggcgagccga ccgtggtcga aaccaccggg cgccatgacc cttgcgtggg catacgcgcc
     3061 acgcccatcg ccgaagcgat gctggcgctg gtgctgatgg accatgcgtt gcgccatcgc
     3121 gcgcagaatg ccgacgtgcg ttgccctaca cccaggattg ccgcgcgcgc tccggccggg
     3181 gcagcgagcg cgggcaatcc gagccaggca accgatcccg accccgacga agcgtaaggc
     3241 cgatcacccc agtgagctgg ctgcagcgcc tcgccgaggt caatgaacag gactggctcc
     3301 cgctagggac cccggcgttt gttgcggcca ccctcggcat cgcgcttgtt gctttcctcg
     3361 ccaacaccgg ccagcgttgg gttttcctgc tcgacaacgc caatctcgcc ttgcacgaag
     3421 ccggccaccc gatcttcggc ctgcttctgg gcgatcgcat caccgtttat ggcggcacgc
     3481 tcgcgcagct gctgtttccg gttgccgcag ccgcgacgtt ctggcggcgc cgcgaggcgc
     3541 tgtcgttcgc gctgtgcgcg gtgtggctgt tcgagaattt ctggaacatc gcccgctaca
     3601 tggccgatgc gcgcgcgcag gtgctgccgc tggtcggcag cggcgagcac gactggaccg
     3661 agatcttttc ccgctggggc gtactgggcc gtgataccgg catcgccggt ttcgttaccc
     3721 tcctgggctg gctcggcgtg ctgatcgcct ggggctggct ggcgcggcgc tggtggctcg
     3781 agcgcgagct cggacagcgc tgagggggtc gggttagtcc atggcgcgcg gatcgtttta
     3841 cttccggctt tccaatttct atttctggta cttcgcattc atcggcgcgt tcgcgccgta
     3901 tttcgcgttg tatctgaaat cgctgggatt ctccgcttcc cagatcggcg tgctgcttgc
     3961 cgtcaatccg gtggcgcgcg tcctcggacc gaatatctgg gggtggctgt cagaccatta
     4021 cggtgcgcgc ggccagctga ttcgtctgac cgcggtggga acggcggtcg cgttcgtcgc
     4081 ggtgttcttc aaccgcggaa tcggctggat gttcaccgcg ttgctggtgc tgaacatttt
     4141 ctggtgcgcg gtgctgccgt tggcggaatc ggccacgctc aacctgctgg gctcgagggt
     4201 cggcacttac gggcgcattc ggctgtgggg ctcggtcggg ttcgtggcgg tggtcgtcgc
     4261 cggcggctat tgtctcgatt acttcggtat caactcgctt gcgccgatgg ttctgacgct
     4321 gctggtgctg atggcggcga gcacctttcc gctgccgcgc gaccgcgaac cttcgcacca
     4381 cggcgagcat gcgccgattc tgcgcatcgt gacgcagccg gcggtcattg cgctgtttgc
     4441 cggatttttt ctcatgcagg tggcgcacgg gccgtacaac ggcttttact cgatccacct
     4501 ggtcgaggcc ggctactcga aaaaggcggt gggctggttg tgggcgttgg gtgtgatggc
     4561 cgaaatcgga ttgtttatgt ggctcccgcg cctgatgcgg gcgtacagcc tgaaccagat
     4621 tctgctgttc agtttcggct gtgcgttcgc gcgttttctg ctgatcgcct ggggggtcgg
     4681 ttcgattccg gtcatggtgg tggcgcagct catgcacgcg atcactttcg gcgcttatca
     4741 cgccgccggc gtggcggcaa tgcaccagat ctttcgcggc cgcaaccagg cgcgcggcca
     4801 ggcgctgtac accagcctcg gctacggcct gggcggcacg ctgggcacgc tcggcgccgg
     4861 ctatgcctgg gacggctggg gtccgcagtg gactttcacg tttgccgcag tcgccgcgtt
     4921 gctggcgttc ctggtggtgt catggaagcc gatgtccttc ggcacaaagc gggaaatcta
     4981 gtcgcgccaa actgccgtcc gaccagcggg atatgtcata atttccgctt gctctgaagg
     5041 cgccatgaat ccgcagactc tttacgacaa actctgggcg agccacctgg tccggcagga
     5101 accggatggc accgcgctga tctacatcga ccgccacctg gtgcacgaag tcaccagccc
     5161 gcaggccttc gaagggctca agctcgccgg tcgcaagcct tggcgcatcg actcggtggt
     5221 ggcaaccgcc gaccacaaca cgccgaccac ggactgggac aagggaatca gggatccgat
     5281 ttccaggttg caggtcgaga cgctggacgc caacattcgc gagttcggcg ccaaggcata
     5341 cttccccttt ctcgatcgcc gccaggggat cgtgcatgtg atcggcccgg agcaaggggc
     5401 cacgctgcca gggatgaccg tggtgtgcgg cgattcgcat accagcactc atggcgcgtt
     5461 cggttgtctt gcccacggca tcggcacctc ggaggtggag cacgtgctcg ccacccagtg
     5521 cctgatgcag aaaaagatga agaacatgct gatcgaggtg aacggcgcgc tcggccgcgg
     5581 cgtcaccgcc aaggacgtgg cgctggcggt gatcggcaaa atcggcacgg cgggcggcag
     5641 cggccatgcg gtcgagttcg gcggcagcgc ggtacgcgcg ctgtcgatgg aagcacgcat
     5701 gaccctgtgc aacatggcga tcgaagccgg cgcccgcgcc gggatggtgg cggtggacga
     5761 gaccaccatc gagtatctgc gcggccgtgc gtttgcgccc ataggcaaga tgtgggaccg
     5821 ggcggtgacc cattggcgca cgttgaaaag cgacgacaac gcccgtttcg attttgtggt
     5881 gcggctcgat gcccgcgaga tcaagcctca ggtgacctgg ggcacctcgc cggaaatggt
     5941 ggtgccggtg gacggccgag tgcccgatcc ggacaaggaa aaggacgcgg tcagacgcga
     6001 gggcatggag cgggcgctga cttacatggg cctcaagccc aatacccaga tcaccgatat
     6061 ccgcgtcgac aaggtgttca tcggctcatg taccaactcg cgcatcgagg acctgcgtgc
     6121 cgcggcgcaa atcgtgcgcg gcaggcgcgt tgccgccaat gtacgcctgg cgatggtggt
     6181 gccgggttca ggcctggtca aggaacaggc ggagaaagaa ggactggacc gggtgtttcg
     6241 cgatgccgga ttcgagtggc gccaaccggg ttgctcgatg tgcctggcga tgaacgacga
     6301 ccggctcgaa ccgggcgaac gctgcgcctc gacctcgaac cgcaacttcg agggacgcca
     6361 gggcgcgggt ggccgcacgc acctggtgag tccggcgatg gcggcgggcg cggcgatcgc
     6421 cggccacttt gtcgacgtgc gctcgggact gtagagaccg cgatccggga gcaatatgga
     6481 gaagttcacc cgactggaag gcctggctgc gccgctcgat cgcgccaacg tcgacaccga
     6541 cgccatcatt ccgaagcagt tcctgaagtc gatcaggcgt tcggggttcg gcgtcaatct
     6601 gttcgacgcg tggcgttatc tcgatcacgg cgaacccggg atggatcctg tgacgcgcaa
     6661 gcccaatccc gatttcgtcc ttaaccagcc gcgctacagg ggcgccaaca ttcttcttgt
     6721 gcggaagaat ttcggttgcg gctcctcgcg cgagcacgca ccctgggcgt tgggcgactt
     6781 tggtttccgt gcgctgatcg cgccgagtta cgccgacatc ttttacaaca attgcttcaa
     6841 gaacggcctg ctgccggtcg tgctgcctga atcccaggtt gatcgcctgt ttcacgacat
     6901 cgccgcgttc cccggattca ggctggtgat cgatctcggc gcgcagaccg tcgcttatcc
     6961 ggacggcagc caggtgttca ctttcgaaat cgattccttc cgcagacatt ccctgctaaa
     7021 cgggctggac gatatcgggc tgacgctgcg tcatgtcgac gagatcaagg ccttcgaggc
     7081 caaacgccgc gtcgagcagc cctggctatt cagctagggt taagggtgaa gagtgagggg
     7141 tgaagggtaa aaccttcgca ttgccctgct tgccccaaag cattcactcc ttcacgcatt
     7201 cacggaatca atcatgaaaa tcgcagtact ccccggtgac ggcatcggcc ccgagatcgt
     7261 gaaacaggcg ctcaaggtgc tcagggcgct gtcgcgcgaa ggccagaaat tcgatctgca
     7321 ggaagcgccg atcggcggcg ccggttacga tgccggcgga gatcccttgc cggaatcgac
     7381 cctggcgcta gccaagtccg cggacgcggt gctgctgggc gccgtgggcg gcccgaagta
     7441 cgataaattg cctcggccga tgcgccccga gcagggcttg ctgcgcatac gcaaggaact
     7501 gggcctgttc gccaacctgc gcccggcgct gctgtacgag gagcttgcca atgcctcgac
     7561 gctcaagtcc gacgtggtgg cggggctcga tatcatgatc ctgagggagc tgaccggcga
     7621 catctacttc ggccagccgc gcgggcgacg cgagaccggt agcggcgagc gcgagggtta
     7681 cgacaccatg agatacggcg aatcggagat tcgccgcatc gcccatgtcg gtttcggcat
     7741 cgcgcgcaag cgggcgaagc gcttgtgttc agtggacaag gccaacgtgc tcgacacgag
     7801 catcctgtgg cgcgaggtgg tcaatgaagt ggcggaggag tatccggacg tcgagctgtc
     7861 gcacatgtac gtggacaa
//
LOCUS       DASQOP010000289         8408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1356637, whole genome shotgun sequence.
ACCESSION   DASQOP010000289 DASQOP010000000
VERSION     DASQOP010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8408
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356637"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VE030_11650"
     CDS             <1..352
                     /locus_tag="VE030_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24106.1"
                     /translation="VVGIAVLPGRFLELSGTNASGPVWQNLTIERYRSRMQLDIQPLL
                     IEQHNDLGDGLVRSWSRPDFGVAKHYGYTAQWFSLCGLIVFLYVFFQVRRARSKKDPQ
                     NSAAPEREQNSDWK"
     gene            360..1463
                     /locus_tag="VE030_11655"
     CDS             360..1463
                     /locus_tag="VE030_11655"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HZF24107.1"
                     /translation="MQNSAIWQFSLNALHSAAWGGSLLVALVVCARYLQLRTLVVATT
                     VVTLGLVMLGAYVRLTDAGLGCPDWPGCYGKLSPTHAAAEIRAAEAIAPAGAVSLPKA
                     WNEMVHRYFASFAGVMIVAIAWQTLRRRDKPGTDDPSARIGLPLALVGLVIAQGLFGK
                     WTVTLLLKPAIVTLHLLGGMALVALLAWLSARHLGISGGQASTLRAIRPWAVLGVVIL
                     GAQIALGGWVSANYAGLACVDFPTCHGEWAPAMDFGHGFYFFRELGMTADGQALSNQA
                     LNAIHWAHRVGALVTFAYLAVLGHSAMRLRGLRRIGIAILVLLLVQVTLGIANVLASL
                     PLPVAVAHNGVAALLLAALVMLNFAACSPSSFH"
     gene            1467..2375
                     /gene="cyoE"
                     /locus_tag="VE030_11660"
     CDS             1467..2375
                     /gene="cyoE"
                     /locus_tag="VE030_11660"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HZF24108.1"
                     /translation="MSISLPSQTQLSRIHQLLQLTKPRVVSLIVFTAVIGMFLAVPAG
                     RLVSPVFWFAALFATLGIALVAGSAAAVNCLVEQKIDAKMARTRARPLPMGTITSLET
                     LLFAGVVGGFGLTMLYLLVNPLTMWLTLATFVGYAVIYTVLLKPLTPQNIVIGGASGA
                     MPPVLGWAAVTGEVASDALLLFLIIFAWTPPHFWALALYRKNEYAKAGVPMLPVTHGD
                     RFTRLHVLFYTVILVAVTALPFASRMSGLLYLASAVALGALFLSYAVRIFVAYSDELA
                     KKTFRYSIVYLSLLFAALLVDHYLRV"
     gene            2431..2976
                     /locus_tag="VE030_11665"
     CDS             2431..2976
                     /locus_tag="VE030_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HZF24109.1"
                     /translation="MLGACQQGRPGFRATDITGADFGRDFALTDHTGKPRSLADFRGR
                     VVVMFFGFTHCPDVCPTTLAELAAVVKKLGTDGDRVQVLLVTVDPERDTPEVLSQYVT
                     AFDPRFLALRGTAEETARVAKEFKIIYQKVAGARPDSYTMDHSAGTYIFDTQGRLRLY
                     ASYGQGPEIFAHDIALLLKQP"
     gene            complement(3044..3907)
                     /gene="rpoH"
                     /locus_tag="VE030_11670"
     CDS             complement(3044..3907)
                     /gene="rpoH"
                     /locus_tag="VE030_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HZF24110.1"
                     /translation="MTNALTLAVPFPSTTGSLDTYVQAVNRYPLLTQEQETALARRLR
                     DEGDVNAARELVLSHLRVVVSIARGYLGYGLPHADLIQEGNVGLMKAVRRFDPDRGVR
                     LVSFAVHWIRAEIHEFILKNWRIVKVATTKAQRKLFFNLRSLKQGHSTMSPEAVADMA
                     RRLNVRAEDVVEMETRMGGHDVALEPSSDDEDESFAPITYLADPTDEPGLVLEHDQSE
                     RARSQGLEQALAALDPRSRRIIEARWLREQDTATLHELADEFGVSAERIRQIEGQAMK
                     KMRALMAPSAS"
     gene            complement(4011..4916)
                     /gene="ftsX"
                     /locus_tag="VE030_11675"
     CDS             complement(4011..4916)
                     /gene="ftsX"
                     /locus_tag="VE030_11675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HZF24111.1"
                     /translation="MNWLLAHFDTLRESLVRLARQPFATGLNVIVIGIALSLPVGFYL
                     GIDNLQAFSRQLSSDPQVSIFMAMDAGQADVSAVERRLRAHPEVGRVDFVAREQALAK
                     LKRSAGLADVLADLERNPLPDAFVVTARSNDPATLEALRDQAARWPKVEHVQLDAEWA
                     RRLDAALGVGRMLVTLLAALLALALVAVTFNTIRLQILTRRDEIEVSKLIGATNPFIR
                     RPFLYLGALQGLGGGCAAWVIVTLAMIVLNVQLAELTTLYGTAFKLQPLTAADSAVLL
                     LFSAALGWLGAWLSVSRHLWQIEPQ"
     gene            complement(4913..5566)
                     /gene="ftsE"
                     /locus_tag="VE030_11680"
     CDS             complement(4913..5566)
                     /gene="ftsE"
                     /locus_tag="VE030_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HZF24112.1"
                     /translation="MISVSHVSKRYPGGYEALRDVTFTVEPGEMVFITGHSGAGKSTL
                     LKLIAAIEMPSSGGVIINGQNVSAMKSGAIPYLRRNFGLIFQDHKLLFDRSVFENVAL
                     PLAITGFPRGEIARRSRAALDKVGLLGREKARPITLSGGEQQRLCIARAIVNRPAILL
                     ADEPTGNLDAAYAEEIIGMFKSFNQVGVTVVIATHDEALVGRLGPRILQLDHGRLAA"
     gene            complement(5673..6635)
                     /gene="ftsY"
                     /locus_tag="VE030_11685"
     CDS             complement(5673..6635)
                     /gene="ftsY"
                     /locus_tag="VE030_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HZF24113.1"
                     /translation="MLSFLKRDKDEPIKDGGDGSWAARLRRGLAATRSRLGDQLAVVF
                     GSGRKVDEQLFEDLEAVLLSCDVGVEATHHLLDQTRARAQRERLQDATQIRDALRQTL
                     LELLAPLAQPLDLSRAKPFVILIAGVNGAGKTTSIGKLAGFFRNQGKSVLLAAGDTFR
                     AAAHEQLKIWGERSDVVVIAQDGGDTAAVIFDAVQSALSKGIDVVIADTAGRLPTQPH
                     LMEEIRKVKRVIAKVIPEAPHEVLLVLDANTGQNAVAQVKAFDQALGVSGIILTKLDG
                     TAKGGAIAAIARHKPIPLRFVGVGEGMDDLRPFSAEQFVQALFE"
     gene            6714..8090
                     /locus_tag="VE030_11690"
     CDS             6714..8090
                     /locus_tag="VE030_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZF24114.1"
                     /translation="MLDNLAVARWALLASALVCVAPVGATAGSAERTLANGLKVVVKE
                     DHRSPVVVAMVWYRAGSMDEVSGTTGVAHVLEHMMFKGTQRVAGGEFSRIIARAGGRS
                     NAFTSRDHTAYHEQLHKSKLALALELEADRMVNLQFSAEEFGKEMKVIMEERRMRTDD
                     DPHAQLSETMMATIYAVHPYRTPVIGWMNDLENMRLADAREWYEKWYAPNNATLVVGG
                     DVEAEEVFRLAGKFFGPIPARALPQRKSQVEPPQRGVKRVNLKAPAEQPYLLMAYHVP
                     VLRDVDNDWEPYALSVLGGVLDGSAAARLPKELVKNSRIANSAGSSYDIANRGPGLFL
                     LDGIPAEGKSAGEVETALRDQIRRLVEEGISEDELRRVKAQVIAAQVFAQDSVFYQAM
                     RIGALDTVGLPHDSVDRQMKKLQEVSAAQVREVARKYLVDDNLTVAVLDPQRVATGAP
                     APEGGKRP"
     gene            8087..>8408
                     /locus_tag="VE030_11695"
     CDS             8087..>8408
                     /locus_tag="VE030_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HZF24115.1"
                     /translation="MTVRPQVLAGLLAVATVTCAAPAYATLPIQQWQTAGGARVLFVE
                     THDLPMLDVAVDFPAGSSRDTREKSGLAALTLRMLRLGAEGLEEDQIAERLADVGADI
                     GGRFD"
BASE COUNT         1437 a         2625 c         2753 g         1593 t
ORIGIN      
        1 ggtcgtcggc atcgcggtgt tgccggggcg tttcctggaa ttgtccggga cgaatgcaag
       61 cggaccggtg tggcagaacc tgaccattga gcgctatcgc agccgcatgc aactcgacat
      121 ccagccgctg ttgatcgagc agcacaacga cttgggcgac ggcctggtgc gctcatggag
      181 ccgccccgat tttggcgtcg ccaagcacta cggatacacg gcgcaatggt tttcgctgtg
      241 tggactgatt gtttttctct acgtgttctt tcaggtcaga cgcgcccgct ccaaaaagga
      301 tccgcaaaat tccgctgctc ctgagcgcga gcagaacagc gactggaagt aggcaaggaa
      361 tgcagaattc ggcaatctgg cagttttccc tgaatgcgtt gcactcggcc gcgtggggcg
      421 ggtcgctgct ggtagcgctg gtcgtgtgcg cacgctacct ccagctgcgc acgctggtgg
      481 tcgctaccac ggtggtgacg ctcgggctgg tcatgcttgg cgcctacgtg cggctgaccg
      541 acgccgggct tggctgcccg gactggcccg gctgctacgg caagctgtcg ccgacgcatg
      601 cggcggcgga aatccgcgct gccgaggcga tcgcgcccgc cggtgcggtg tcgctgccca
      661 aggcctggaa cgagatggtg catcgctact ttgcctcgtt cgccggcgtg atgatcgtgg
      721 ccattgcctg gcaaacactg cgccgccgcg acaagcccgg aaccgatgat ccgtccgcac
      781 ggatcggttt gccgctggcg ttggtgggcc ttgtaatcgc tcagggattg ttcggcaagt
      841 ggacagtaac gctgctgctc aagccggcca tcgtgacgct gcacctgctg ggcggcatgg
      901 ccctggtggc gctgctggca tggctgagcg ctcgccatct tggaatcagc ggcgggcaag
      961 cgtcgaccct gcgcgcgatc cgtccctggg cggttctggg tgtggtgatt ctgggcgcgc
     1021 agatcgcgct cggtggctgg gtcagcgcca attacgcggg gctggcctgt gtggacttcc
     1081 cgacctgcca tggcgagtgg gcaccggcga tggacttcgg gcacggattc tattttttcc
     1141 gtgagctggg catgactgcc gacggccaag cgttgtcgaa ccaggcgctt aatgcaatcc
     1201 actgggcgca tcgcgtcggc gcgctggtga cctttgcgta tctggccgtc ctggggcact
     1261 cggcgatgcg gctgcgcggc ctgcgccgga tcggtatcgc gatactggtg ctgctgctcg
     1321 tccaggtgac gctcggcatc gccaatgtcc tggcgagtct gccgctcccc gtggcagtgg
     1381 cccacaacgg cgtggcggcg ctgttgctgg cggcgctggt gatgctaaac tttgccgcct
     1441 gttcgccgtc cagcttccat tagcagatgt cgatttccct tccttcccag acacagttgt
     1501 cgcggataca ccagttgctg cagctcacca agccgcgcgt ggtatcgctg atcgtcttca
     1561 ccgcggtgat cggaatgttc ctggcggtgc cggccggacg gctggtgtcg cccgtgttct
     1621 ggtttgcggc gctgttcgcg acgctgggca tcgcgctggt ggcgggttcg gccgcggcgg
     1681 tcaattgtct ggtcgagcag aagatcgacg ccaagatggc gcgcacccgc gcccgtcccc
     1741 tgccgatggg caccatcaca tcgctggaaa cgctgttgtt tgccggcgtc gtgggcggtt
     1801 tcggattgac gatgctgtac ctgttggtca atccgctcac catgtggctg acgctggcca
     1861 cgttcgtcgg ctacgctgtc atctacaccg tgctgctcaa acctctcacg ccacagaaca
     1921 tcgtgatcgg cggtgcctcc ggcgcgatgc ccccggtgct cggctgggcg gcggtgaccg
     1981 gggaagttgc ctccgacgcg ttgctgctgt tcctgatcat tttcgcctgg accccgccgc
     2041 atttctgggc cctggcgctg taccgcaaga acgagtacgc gaaggccggc gtcccgatgc
     2101 tgccggtcac ccatggcgat cgcttcactc gcctccacgt gctgttctac accgtgatcc
     2161 tggtcgcggt gacggcgctg ccattcgcca gccgcatgag cggcctgctc tacctggcca
     2221 gtgccgtggc gctgggcgcg ttgtttctaa gctacgccgt ccggattttc gtggcctaca
     2281 gcgacgaact ggcgaagaaa accttccgct actccatcgt gtacttgtcg ctgttgtttg
     2341 cggcgctgct ggtggatcac tacctccgcg tctgagcggg agctcgccat gaaaccggcg
     2401 ttgcgcctgc tcctgctggt cctgctgctc atgcttggcg cctgccagca aggcaggccc
     2461 ggttttcgcg ctaccgacat taccggagcc gattttggcc gggactttgc gcttaccgac
     2521 cacaccggca agccgcggtc gctggcggat tttcgcggcc gggtggtggt gatgttcttc
     2581 ggattcacgc attgcccgga cgtgtgtccc accacgcttg ccgaactggc ggcggtggtg
     2641 aaaaagctcg gcaccgacgg ggacagggtg caggtgctgc tggtgaccgt ggacccggag
     2701 cgcgatacgc ctgaagtcct gtcgcaatat gtgaccgcat tcgatccgcg tttcctggca
     2761 ttgcgcggca ctgccgagga aaccgcgcgg gtggcgaagg aattcaagat catctaccag
     2821 aaagttgccg gcgcgcgccc cgatagctac accatggatc attccgcagg cacttacatt
     2881 ttcgacacgc agggacggct gcgcctgtat gcgagttatg ggcaagggcc ggagattttt
     2941 gcccacgaca tcgccttgtt gttgaaacag ccgtagagcg cacggccaaa aagaaaacgc
     3001 cccgcgtccg cggggcgttt tcctgtggaa ctgcgtaacc gggtcaggag gccgatggcg
     3061 ccatcagcgc gcgcattttc ttcatcgcct ggccctcgat ctggcgtatg cgctcggcgg
     3121 aaacgccgaa ttcgtcggcc agttcgtgca gggtcgccgt atcttgctcg cgcagccagc
     3181 gcgcctcgat gatgcggcgg ctgcgggggt cgagcgcggc cagcgcctgt tccagcccct
     3241 ggctgcgggc gcgttcggac tggtcgtgtt cgagcaccag gccgggctca tcggtggggt
     3301 ccgccagata ggtaatgggc gcgaagcttt cgtcttcgtc atcggacgac ggctcgagcg
     3361 ctacgtcgtg cccacccatc cgggtctcca tctcgaccac gtcttcggcc ctgacgttga
     3421 gccgtctggc catgtctgcc accgcctcgg ggctcatggt cgagtggccc tgcttgaggc
     3481 tgcgcaggtt gaagaacagc ttgcgctgcg ccttggtggt ggccactttg acgatgcgcc
     3541 agttcttcag gatgaattcg tggatctcgg cgcggatcca gtgcaccgcg aacgacacca
     3601 gccgcacgcc gcggtcggga tcgaagcgcc ttaccgcctt catcaggccg acgttgcctt
     3661 cctggatcag gtcggcgtgg ggcaatccgt aacccagata gccgcgggca atcgacacca
     3721 ccacgcgcag gtgcgacagc actaattcgc gcgccgcatt cacatcacct tcatcgcgca
     3781 ggcgccgggc tagcgcggtc tcctgctcct gggtcaggag agggtagcgg ttgaccgcct
     3841 gaacataagt atccaggctg cccgtcgtcg acgggaacgg aaccgcaaga gtcaatgcat
     3901 tagtcattgg gtattcctcc ttgcaacgac aattttcgca aatgttagca ctctggctgt
     3961 gggagtgcca atagcctccg gaagttcccg aacatggcgg ccagagtcct ttactgtggc
     4021 tcaatctgcc ataagtggcg acttactgac agccaagcgc caagccaccc tagtgcggcc
     4081 gaaaagagca gcagcacggc tgagtcggcg gcagtcaggg gttgcagctt gaacgccgtg
     4141 ccgtaaagcg tggtcaactc ggcaagctgg acgttgagta cgatcatcgc caaggttacg
     4201 atgacccagg cggcacagcc gccgcccaga ccctgtagcg cacccagata caggaagggc
     4261 cggcggatga aagggttggt ggcgccgatc agcttggaaa cttcgatctc atcgcgacgg
     4321 gtgagaatct gtaggcggat ggtgttgaac gtcaccgcca ccaaagccaa cgccagcagc
     4381 gccgccagca acgtgaccag catgcgtccc actccaagcg cggcatccag ccgccgtgcc
     4441 cattcggcgt ccagctgtac gtgctcgacc tttggccagc gcgcagcctg gtcgcgcagc
     4501 gcctccagcg tggccgggtc gttgctcctg gccgtgacca cgaaggcgtc gggcagcggg
     4561 ttgcgctcca ggtcggccag cacgtccgcc agtccggcgc tgcgcttgag cttggctagc
     4621 gcctgttcgc gcgcgacgaa gtcgacgcgt ccgacttcgg gatgggcccg cagacgccgt
     4681 tccactgccg acacatcggc ctggccggca tccattgcca tgaagatgct gacctgcggg
     4741 tcgctggaaa gctggcgcga aaacgcctgc agattgtcga tgccgagata aaaaccgacc
     4801 ggcaaagaca acgcgatgcc gatcacgatc acattcaacc cggtggcgaa aggctggcgc
     4861 gccagcctga ccaggctctc gcgcagcgta tcgaagtggg ccagcagcca gttcatgcgg
     4921 cgagcctgcc gtggtcgagc tgcaggatgc gcggaccgag gcggccgacc agcgcctcgt
     4981 cgtgggtggc aattaccacg gtcacgccga cctgattgaa cgacttgaac atgccgatga
     5041 tctcttcggc ataggccgcg tcgagatttc cggtaggctc atcggccagc aggatggccg
     5101 ggcggttgac gatggcgcgg gcgatgcaga gcctctgctg ctcgccaccc gagagcgtga
     5161 tcggcctggc tttctcccga cccagcaggc cgaccttgtc gagtgccgcg cgcgagcgcc
     5221 gcgcgatctc gccgcgcgga aagccggtga tcgccagcgg cagcgccacg ttctcgaaca
     5281 cgctgcgatc gaaaagcagc ttgtggtcct ggaaaatcag tccgaaattg cggcgcagat
     5341 agggaatggc gccggacttc atcgccgaga cgttctggcc gttgatgatc acgccgccgg
     5401 agctcggcat ctcgattgcg gcgatgagct tcaacagcgt gctcttgcca gcgcccgagt
     5461 gcccggtaat gaacaccatc tcgcccggct ccacagtgaa agtcacgtcg cgcagcgctt
     5521 cgtaaccgcc ggggtagcgc ttggaaacgt ggctgacgct gatcatgcgc gaggctgtca
     5581 tgggaatccg ggcggagcca agggcgcgga aagcgaatca ggaccgcgcg accgcaacgc
     5641 ccggccatcc ttgctccaca cccggcccca catcattcga acaatgcctg cacgaattgc
     5701 tcggccgaga acgggcgcag atcgtccatg ccctcgccaa cacccacgaa acgcagcgga
     5761 atcggtttgt ggcgggcaat ggcggcgatg gcgccgccct tggcagtgcc atcgagcttg
     5821 gtcaagatga ttccgctcac ccccagcgcc tggtcgaaag ccttcacctg cgcgaccgcg
     5881 ttctgtccgg tgttggcatc cagcaccagc aggacttcgt gcggtgcctc cgggatcacc
     5941 ttggcgatca cccgtttcac cttcctgatc tcttccatca ggtgcggctg ggtcggcagc
     6001 cgtcccgcgg tatcggcgat cacgacatcg atgcctttgg acaacgccga ctgaacggca
     6061 tcgaaaatga cggccgcggt atcgcccccg tcctgagcga tgacgacgac atcgctgcgt
     6121 tcgccccaga tcttcaattg ttcgtgcgct gcggcgcgaa aggtgtcgcc ggccgccaac
     6181 aggactgatt ttccctggtt ccggaaaaag ccggcgagct tgccaatcga agtggtcttg
     6241 ccggcgccat tgacgccggc gatcagtatc acgaagggtt tggcgcgaga cagatcgagc
     6301 ggttgcgcca gcggcgcaag cagctcaagc agcgtctgcc gcaacgcatc gcgaatctgc
     6361 gttgcgtcct gcaggcgctc gcgctgcgcg cgcgcgcgcg tctgatcgag aaggtggtgg
     6421 gttgcctcca cgccgacgtc gcaggacagc agtaccgctt cgaggtcctc gaagagctgc
     6481 tcgtccactt tgcggccgct gccgaagacc acggcgagct ggtcgccgag ccgcgagcgg
     6541 gtggccgcca gcccccggcg cagccgggcg gcccagctgc cgtcgccgcc gtcttttatc
     6601 ggttcgtcct tgtcacgctt gaggaaactc aacatactct ggcgctgtca cagcagcact
     6661 cgaaatcaga cagccgcaat tctattctgt ttgttcttgg ctcatccata agcatgctcg
     6721 ataaccttgc tgtcgcgcgc tgggcgctgt tggcgtcggc gctggtctgc gtggccccgg
     6781 tgggggccac ggccggctcg gcggaacgca cgctcgccaa cggcctgaag gtcgtggtga
     6841 aggaggatca tcgttccccg gtggtggtgg ccatggtgtg gtatcgcgcc ggcagcatgg
     6901 atgaagtcag cggcaccacc ggtgtggcgc acgtactcga gcacatgatg ttcaaaggca
     6961 cgcaaagagt ggcgggtggc gaattttccc ggatcattgc gcgcgccggc ggccgcagca
     7021 acgcgtttac ctcgcgcgac cacaccgcct accatgagca actgcacaag tcgaagctgg
     7081 cgctggcgct ggagctcgag gcagaccgca tggtgaatct gcagttctcg gcggaggagt
     7141 tcggcaagga aatgaaagtc atcatggaag agcggcgcat gcgcaccgac gacgatccgc
     7201 atgcacaact ttccgagacg atgatggcga cgatctatgc ggtgcatcct taccgcacgc
     7261 cagtaatcgg ctggatgaac gatctcgaga acatgcgtct cgcggacgcc agggaatggt
     7321 acgagaagtg gtacgcgccg aacaacgcta cgctggtggt gggcggtgac gtggaggctg
     7381 aagaggtttt ccggctcgcc gggaaatttt tcggcccgat tcccgcacgt gcgctgccgc
     7441 agcgcaaatc gcaagtcgag ccgccgcaac ggggcgtgaa gcgcgtgaat ctgaaggcgc
     7501 ccgcggaaca gccttacctg ctgatggcgt atcacgtacc agtgctgcgc gatgtcgaca
     7561 acgattggga gccgtacgcg ctgtcggtgt tgggtggcgt gctcgacggt tctgccgcgg
     7621 cgcggcttcc caaagaactg gtgaagaata gccgtatcgc caattccgcg ggttcgagct
     7681 acgacatcgc caaccgcgga cccgggctgt tcttgcttga tgggattccg gcagagggca
     7741 aaagcgcggg cgaggtcgag acggcgttgc gcgaccaaat ccgccgcctg gtggaagagg
     7801 gtatcagcga agacgagctg cgccgggtca aggcacaggt cattgcggca caagttttcg
     7861 cgcaggattc ggtgttctac caggcgatgc gcatcggcgc gttggatacg gtcggattgc
     7921 cccacgattc ggtcgaccgg cagatgaaga aactgcagga agtcagcgct gcacaggtgc
     7981 gcgaagtggc gcgcaagtat ctggtggacg acaacctgac ggtggcggtg ctcgatccgc
     8041 agcgggtcgc caccggcgcg cccgctcccg aaggcgggaa acggccgtga cggtcagacc
     8101 gcaagtgctt gcaggcctgc tcgctgttgc tacggttaca tgcgcggcgc cggcgtacgc
     8161 cacgctgccg atccagcagt ggcagacggc gggtggcgcc cgcgtgctat ttgtcgagac
     8221 ccatgatctg ccgatgctgg acgtggccgt ggacttcccg gccggaagca gccgtgatac
     8281 ccgcgaaaaa tccggcctgg ctgccctgac cttgcgcatg ctgcgattgg gcgccgaagg
     8341 cctcgaagag gaccagattg ccgagcgtct cgccgacgtc ggcgcggata tcggcggacg
     8401 attcgaca
//
LOCUS       DASQOP010000290         2497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2570277, whole genome shotgun sequence.
ACCESSION   DASQOP010000290 DASQOP010000000
VERSION     DASQOP010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2570277"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2486)
                     /locus_tag="VE030_11700"
     CDS             complement(<1..2486)
                     /locus_tag="VE030_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010394056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HZF24116.1"
                     /translation="MQEVTLDDKYASDQGRVYLTGIQALVRLALVQRRRDLAAGLNTA
                     GFISGYRGSPLGGLDEALWKAAKHLEAHHVKFQPGVNEELAATAVWGTQQVHLIGDSD
                     YDGVFAMWYGKGPGVDRCGDVFKHMNFAGTSKHGGVLLVAGDDHGAYSSTLPHQSDHL
                     FSASMIPVLYPCNVHEYIELGLHGWTMSRFSGCAVGFKALADTVESSASIEADPFHVQ
                     TLLPEDFVLPEGGLNSRLSSNLLGLQARAQEALMQDYKIYAALAYSRKNKLNRTTIDS
                     PNARLGIIASGKSYMDVLEALEELGITERNAAEIGVRLFKVSMPWPLEPDGVREFSKG
                     LEEILVVEEKRQMVEYQLKEHLYNWHPDVRPRVIGKFDDQGEWVHPRGEWLLPAKADF
                     SIAQIARVIAGRIGRFHQSDLIKARLAFLEAKEAVLKKAVNTPVRPPYYCSGCPHNTS
                     TKVPEGSLALAGIGCHVMATVIYPEQNKSPTHMGGEGAPWIGQAPFSKRTHIFANLGD
                     GTYFHSGSLAIRAALAAKVNITYKILYNDAVAMTGGQPVDGEISVPMIAQQMAAEGVG
                     RIAVVTEELARHEDRSQLPALVTLHDRKDMEAVQKELREVRGVSVMIYDQTCAAEKRR
                     RRKKGEYPKATQRVFINEAVCEGCGDCGVQSNCTSILPVETEFGRKRAIDQSSCNQDY
                     SCVKGFCPSFVTIEGGTPAKRAASDLEPPAVAAPEIDLGRPANILIGGIGGTGIVTIG
                     AIVGMAAYLDGRAASVMDQIGLAQKGGEVTSHIRITQSLDALGPVRFAPGEADTLIGC
                     DIAMASNPDVMPLLATDAVAVVNDHVVMTG"
BASE COUNT          434 a          796 c          774 g          493 t
ORIGIN      
        1 tcaccggtca tcacgacgtg gtcattaacg accgcgacgg cgtccgtagc gagcagcggc
       61 atcacgtccg ggttcgacgc catcgcgatg tcgcagccga tcaaggtatc ggcctccccc
      121 ggagcgaacc gcacgggacc cagcgcgtcc aggctttgcg tgatccgaat gtggctggtg
      181 acctcgccgc ccttttgcgc aagcccgatc tggtccatta cggatgcagc gcggccatcg
      241 agataggcgg ccatgccgac gatggcgccg atagtaacga tgccggtccc gccgatgcct
      301 ccaataagaa tattggcagg gcgccccaaa tcgatctccg gcgccgcgac cgcgggcggc
      361 tcaagatcgc tggccgcgcg tttggctggc gtgccgccct cgatcgtgac aaagctcgga
      421 cagaagccct tcacgcacga gtaatcctga ttgcaggacg actggtcgat cgcgcgcttg
      481 cggccgaatt cggtctcgac cggcaggatg gaggtgcaat tggattgcac gccgcagtcg
      541 ccgcagcctt cgcagaccgc ttcgttgatg aatacccgct gcgtggcctt cgggtactcg
      601 cctttcttgc ggcggcggcg tttttccgcc gcacaggtct ggtcgtagat catcacggaa
      661 acgccgcgaa cttccctgag ttccttttgc accgcttcca tgtccttgcg gtcatgcagc
      721 gtcactagcg cgggtaactg cgaccgatct tcgtggcgag cgagttcttc cgtgacgacc
      781 gcgatgcgcc cgacgccttc ggccgccatc tgctgcgcga tcatcggcac cgaaatttcc
      841 ccgtcgaccg gctggccgcc agtcatcgcc acggcatcgt tgtacaggat cttgtaggtg
      901 atgttgacct tggctgcgag cgcggcccgg atcgcaaggg agccggaatg gaaataggtc
      961 ccgtcgccga gattggcaaa gatgtgggtg cgcttcgaga acggcgcctg gccgatccac
     1021 ggcgcgccct cgccacccat gtgcgtcggg ctcttgtttt gctccggata aatcacggtc
     1081 gccatcacgt ggcagccgat gccggccagc gccagactgc cctcgggcac tttggtcgag
     1141 gtattgtgcg ggcatcccga gcagtagtac ggcgggcgca cgggcgtatt caccgccttc
     1201 ttcagcaccg cctctttcgc ctccaaaaag gcaagccttg ccttgatcag atcgctctgg
     1261 tgaaagcgcc cgatgcgtcc ggcgatgacg cgggcgatct gcgcgatgga gaaatcggcc
     1321 ttggcaggga gcagccattc gccgcgcggg tgcacccatt cgccctgatc gtcgaacttg
     1381 ccgatgaccc gcgggcgcac gtcggggtgc cagttgtaga ggtgttcctt gagctggtac
     1441 tcgaccattt ggcgcttttc ttcgacgacc aggatttctt ccagcccctt ggagaactcg
     1501 cgcaccccgt ccggttccag cggccagggc atcgagacct tgaacagccg tacgccgatt
     1561 tcagcggcgt tgcgttcggt aatgcccagt tcctccagtg cttccagcac gtccatgtac
     1621 gacttgcctg aagcgatgat gccgagccgc gcgttgggcg aatcgatcgt ggtgcgattg
     1681 agcttgttct tgcgtgagta cgccagcgcg gcgtagatct tgtagtcctg catcagcgct
     1741 tcctgcgcgc gcgcctgcag gcccaggaga ttgctcgaaa gcctgctgtt cagtccgcct
     1801 tcgggcaata caaagtcttc gggcagcagg gtctggacgt gaaacggatc ggcctcgatc
     1861 gaagcagacg attcgaccgt atcggccagc gccttgaaac cgacggcgca tccggagaac
     1921 cgcgacatgg tccagccgtg caatcccagc tcgatgtact cgtgcacgtt gcaggggtac
     1981 aggaccggga tcatcgaggc cgagaacagg tgatcgctct ggtgagggag cgtggaggag
     2041 taggcgccgt ggtcgtcgcc cgcaaccagc agcacgcctc catgcttcga agtgccggcg
     2101 aagttcatgt gcttgaacac atcgccgcaa cggtccacgc cgggtccctt gccgtaccac
     2161 atggcgaaca ccccgtcgta atccgagtcg ccgatcaggt gcacctgctg cgtgccccac
     2221 accgcagtgg cggcgagttc ttcgttgacg ccgggctgga atttgacgtg atgcgcttcg
     2281 agatgcttgg ctgccttcca cagcgcctcg tcgagtccac ccaatggcga gccgcgatag
     2341 ccggagatga atccggcggt gttgagtcct gccgccagat cgcgccggcg ctggaccaga
     2401 gccaggcgca ccagcgcctg gatgccggtc aaataaacgc gcccctgatc ggaagcgtat
     2461 ttgtcatcca atgtgacttc ttgcatgtct gactcct
//
LOCUS       DASQOP010000291         3468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2571019, whole genome shotgun sequence.
ACCESSION   DASQOP010000291 DASQOP010000000
VERSION     DASQOP010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2571019"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..1537
                     /locus_tag="VE030_11705"
     CDS             59..1537
                     /locus_tag="VE030_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007749167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HZF24117.1"
                     /translation="MQKYHMYVGGEWVAPSSGEWFESYNPFTGEPWAMIARGNAADAD
                     HAVRTAHAAFSDGPWPQLTPTQRGALLRKLGDLVAQNAQHLAEIEVRDNGKLLAEMSA
                     QLNYVPQWYYYFGGLADKVQGSVIPLDKKGYFNFTRYEPLGVVVAITPWNSPLMLTAW
                     KLAPALAAGNTVVIKPSEFTSASALEFVKLVEQAGFPAGVVNVVTGYGKEVGMPLVEH
                     PLTAKIAFTGSDATGRVIAETAARGFKKVGLELGGKSPNIVFEDASMDDAVNGAVSGI
                     FAATGQTCIAGSRLLLQESIHDAFVDKLLALAKTARMGNPMSLETQVGPVTTRPQYDK
                     VLSYIDVAKNEGAKLRMGGGPATRPECGNGWFVEPTIFTGVNNRMRIAQEEVFGPVLS
                     IIPFKDEDDAVRIANDVRFGLGAGVWTQDVGRAIRMSERIKAGTVWVNTYRAVSFMSP
                     FGGYKDSGMGRENGASAIYEYLQMKSVWINTGVKTGNPFVMR"
     gene            complement(1579..2496)
                     /gene="rssA"
                     /locus_tag="VE030_11710"
     CDS             complement(1579..2496)
                     /gene="rssA"
                     /locus_tag="VE030_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HZF24118.1"
                     /translation="MTKNHKRRIGLALGGGSARGWSHIGVIRALQEAGVEPDIVCGTS
                     IGSLVGAAYAAGELDRLEGWVKSLTWQNVVSLLDVTVTGGFIKGAKLVEFFASHFTDV
                     DITKLPKPFGAVATDLLTGREVWLREGSVIESVRASIAVPGLFTPVARDGRLLADGGL
                     VNPVPVSLCRAMGADIVIAVDLNADMVGRSFRKPVEAKDIKPPRRAGRVAFIFDRLQA
                     SLAVIRSGPKSSNSMPSVLDVLTNSINIMQMHIARSRLAGEPADVLIMPRLSQMALLD
                     FHRAAPAISEGSRATNAVLSQLTHLLEEK"
     gene            complement(2655..3281)
                     /locus_tag="VE030_11715"
     CDS             complement(2655..3281)
                     /locus_tag="VE030_11715"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZF24119.1"
                     /translation="MRPGRLGVTQATLPAVVSLHGGNAVVIRQVEAGDAQALQQFFCN
                     LSPASRYRRFLRAIRELPEDMLARFTHPDPAGEAVLVASLPHSRTRIIGMAQYAGTGD
                     SSEIAVVVGDAWQRQGLGYHLLATLMNVAVEAGIERIHADVFADNQPMRRLASKLGSR
                     ISTNPLAPFLVQMTKTLAPGEHGREPGFAPRLGAPIPERMSPAMRAAR"
     gene            complement(3257..>3468)
                     /locus_tag="VE030_11720"
     CDS             complement(3257..>3468)
                     /locus_tag="VE030_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HZF24120.1"
                     /translation="SLWITQECDVKDSVISAGESFTVRNPGLTLLTALSASELTIIEP
                     RAASQPEPWHRASSRVQQCDPAVLA"
BASE COUNT          662 a         1066 c         1145 g          595 t
ORIGIN      
        1 gccctctcca gagaatggag agggagttga gattcaactg cggaaacgag acaccactat
       61 gcagaaatat cacatgtatg tcggcggcga gtgggtggcg ccgtcgtcgg gtgaatggtt
      121 cgagagctac aacccgttca cgggcgaacc ctgggcgatg atcgcgcgcg gcaacgccgc
      181 cgacgcggat catgcggtgc gcaccgcgca tgccgcgttc agcgacgggc cctggccgca
      241 gttgacgccg acccagcgcg gcgcgctgtt gaggaagctg ggcgacctgg tggcgcagaa
      301 cgcgcaacat ctggccgaga tcgaggtgcg cgacaacggc aagctgctcg ccgagatgtc
      361 cgcccagctc aactacgtgc cgcagtggta ttactacttc ggcggactgg ccgacaaggt
      421 gcaaggctcg gtgattccgc tcgacaagaa aggctatttc aatttcactc gctacgagcc
      481 gcttggcgtg gttgtcgcca tcacgccgtg gaactcgccg ctgatgctca ccgcctggaa
      541 gctggctccg gcgctggccg cgggcaatac ggtggtgatc aagccttcgg agttcacttc
      601 ggcatccgca ctcgaattcg tcaagctggt ggagcaggcc ggctttcccg caggcgtggt
      661 caacgtcgtg accggctacg gcaaggaggt gggcatgccg ctggtcgagc acccgctcac
      721 cgcgaaaatc gcattcaccg ggtcggatgc gaccggcaga gtcatcgcgg aaaccgctgc
      781 gcgcggcttc aagaaagtcg gcctggaact gggcggcaag tcgccgaaca tcgttttcga
      841 ggacgcgtcg atggacgatg cggtgaacgg cgcggtgtcc ggcatctttg ccgcgaccgg
      901 gcagacctgc atcgccgggt cgcgcctgct gttgcaggaa tccatccacg atgccttcgt
      961 cgacaaactg ctggcgctgg cgaaaacggc gaggatgggc aacccgatga gcctggaaac
     1021 ccaggtcggt ccggttacca cgcggccgca gtacgacaag gtgctgtcct acatcgacgt
     1081 tgccaagaac gaaggcgcga aactgcgcat gggtggcgga ccggcgaccc ggccggaatg
     1141 cggcaacggc tggttcgtcg agcccaccat ttttaccggc gtgaacaacc ggatgcgcat
     1201 cgcccaggaa gaagtgttcg gcccggtgtt gtcgatcatc ccgttcaagg acgaggacga
     1261 cgcggtgcgc atcgccaacg atgtgcgctt cgggctcggc gccggcgtct ggacgcaaga
     1321 tgtcggtcgc gcaatccgca tgtctgagcg catcaaggcc ggcacggtgt gggtgaatac
     1381 ctaccgtgcg gtaagcttca tgtcgccgtt cggtggttac aaggacagcg gaatgggccg
     1441 cgagaacggc gccagcgcga tctacgagta cctgcaaatg aagagcgtgt ggatcaacac
     1501 cggggtgaag accggtaacc cgttcgtcat gcgctgagcc agaaccggaa gggagtctgc
     1561 cgccgcagcg cccggtccct atttttcctc gagcaagtgc gtcaactggg acagtaccgc
     1621 gttggtcgcg cggctgcctt cgctgattgc cggtgcggca cgatgaaaat ccagcagcgc
     1681 catctgcgac agccgtggca tgatcaatac gtcggcgggt tcaccggcga gccggctgcg
     1741 ggcgatgtgc atctgcatga tgttgatgct gtttgtcaaa acgtccagta ccgagggcat
     1801 ggaattcgag gatttcgggc cgctcctgat gaccgcgagg cttgcctgca gtctgtcgaa
     1861 aataaacgcc acccgaccgg cgcgcctggg gggcttgatg tccttcgctt cgacgggctt
     1921 gcggaaactt cggcccacca tgtccgcatt caggtccacc gcaatcacga tatcggcgcc
     1981 catcgcgcgg cacagggaaa ccggcacggg gttcaccagc cccccgtccg ccagcagccg
     2041 cccatcccgc gccaccggag tgaacaatcc gggcaccgca atggaggcgc gcacggattc
     2101 gatgaccgaa ccctcgcgca gccatacttc gcgcccggtc agcagatcgg ttgcaactgc
     2161 gccgaagggc ttcggcagct tcgtgatgtc cacatcggtg aagtgtgacg caaaaaactc
     2221 tacgagcttg gcgcccttga tgaagccgcc cgtgacggtg acgtcgagca gactcacgac
     2281 gttctgccaa gtgagtgatt ttacccagcc ttcgagccgg tcgagttcgc cggcagcata
     2341 ggcggcgcct accagcgagc cgatcgacgt gccgcagacg atgtcgggct cgacgccggc
     2401 ttcttgcagc gcacgaatca caccgatgtg cgaccagccg cgcgccgagc ctccgccgag
     2461 cgcaaggccg atcctgcgtt tgtgattctt cgtcaaaggc gacggtgtcg cgaggtgtgg
     2521 tgaccttgcg attataaagg ctgaggcggg agaccgaagg acaggaccag gcgtcgggga
     2581 ctcaagaccg agtgaagaac gacctacata tcggcagtga cgcccaagtg ctcagcgctc
     2641 ggcgctcaac cttgtcaacg tgcggcgcgc atcgcaggcg acattcgttc cggaattggc
     2701 gcgccaaggc ggggcgcaaa acccggttca cggccgtgct ccccgggtgc cagtgttttg
     2761 gtcatctgca ccaggaaggg cgcgagcggg ttggtggaaa tccggctgcc caatttggag
     2821 gccaggcggc gcatgggctg attgtcggcg aacacgtcgg cgtgaatgcg ctcgatgccg
     2881 gcctcgaccg cgacgttcat cagggtggcg agcaggtgat agccgagccc ctgccgttgc
     2941 cacgcatcgc cgacaaccac cgcgatctcg ctgctgtcgc cagtgcccgc gtactgggcc
     3001 atgccgatga tgcgcgtgcg ggaatgcggc agcgaagcga ccagcactgc ctccccagcc
     3061 ggatcgggat gcgtaaagcg cgccagcatg tcctcgggca gttcgcgaat cgcccgcaga
     3121 aaacgccgat aacggctggc cggggaaaga ttgcagaaga actgctgaag cgcctgcgcg
     3181 tccccggctt ccacttggcg gatcacgacg gcgtttccgc cgtgcaggct cactacggcc
     3241 ggcaacgttg cttgcgttac gccaagacgg ccgggtcgca ttgctgtacc cgactcgatg
     3301 cgcgatgcca tggttccggt tgcgaggcag cgcgcggctc gatgatggtg agttcggaag
     3361 cgctcagcgc ggtcagcagc gtcaacccgg gattgcgtac ggtgaacgat tcgccggccg
     3421 aaatgacgga gtctttcacg tcgcactcct gcgtgatcca caggctgc
//
LOCUS       DASQOP010000292         4951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1762562, whole genome shotgun sequence.
ACCESSION   DASQOP010000292 DASQOP010000000
VERSION     DASQOP010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1762562"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..690)
                     /locus_tag="VE030_11725"
     CDS             complement(235..690)
                     /locus_tag="VE030_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003276379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HZF24121.1"
                     /translation="MKWITRERPKIDRIACPWLIARFIDPEPEFLFVPSGDVLRIAAA
                     AAAVPYDIPGVELSHVGKLCSFDAFLSKYQLVDPALAQLAVIVRGADTAQLELAPQAA
                     GLLALSLGLSHNFPDDHKMLDHGMVIYDALYAWCRTCQSEAHGWPPKTP"
     gene            complement(706..2064)
                     /gene="chrA"
                     /locus_tag="VE030_11730"
     CDS             complement(706..2064)
                     /gene="chrA"
                     /locus_tag="VE030_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HZF24122.1"
                     /translation="MARSSVNAEAAPAAVSLREALLYWLKLGFISFGGPAGQIAVMHQ
                     ELVERRRWISERRFLHALNYCMLLPGPEAQQLATYLGWLMHGTLGGIAAGALFVLPSL
                     VILIALSWIYMVWGSTPLVAGILYGIKPAVTAIVLYAAWRIGARALRNETLWAISAAA
                     FIAINFFNMPFPAIVLAAGVIGLIGGRVAPQKFATGGAHAESRRDYGPALIDDHTPTP
                     AHAHFRWGGIARVIAVGLSLWAVSMGAIALLFGWANTLSAMGWFFTKAALLTFGGAYA
                     VLPYVVQGAVQHFQWLTPVQMIDGLALGETTPGPLIMVVAFVGFVGAWTHSVFGSESL
                     ALAGIAGAVVATWFTFLPSFLFIFAGAPVIETTHRQPHLTAPLTGITAAVVGVIANLA
                     VFFAVHVFWPDGLRGHLEWPALLIALGAVVALFRYKTGIIPVIAVCGAAGLVVQAART
                     LL"
     gene            complement(2039..3037)
                     /locus_tag="VE030_11735"
     CDS             complement(2039..3037)
                     /locus_tag="VE030_11735"
                     /inference="COORDINATES: protein motif:HMM:NF042524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HZF24123.1"
                     /translation="MNEAKTTALTEDSVTDARRPAWLLFLLTVQGQQSALRMRVWRAL
                     KSLGTAVLRDGVYLLPNRPAFRESLRALEQEVAAAEGNVQVLELDARDAQQQREFEVL
                     FDRTTDYEKLLGEIRDVRKKLPKMDAGAVGTAAARLRREVENLVALDFFPARAASQTT
                     LALKDFLADANAVLSPDEPRPVEGRVRKLDVAEYRGRTWATRKRPWADRLASAWLIRR
                     FIDPEARILWLDKPGDCPEGALGFDFDGATFTHIGSRVTFEVLATSFDLDHDPGVEKI
                     GTLIHYLDVGGVPVPEAVGFEAILRGAQATFTDDDELLREAEKLLNFLYGTFVRQR"
     gene            3202..3792
                     /locus_tag="VE030_11740"
     CDS             3202..3792
                     /locus_tag="VE030_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HZF24124.1"
                     /translation="MKIDTQLARFAVVALLASPIVVTAPAATAKVYDDQETAALVRAL
                     SGSKVSLLEGLHQVGKDDEAAISAKFEFDDNHKLSLSVYTAEKGLAVDAEHNVLKEFS
                     GSPEATAWAPEAEVFKDIPHVARASEHLALMSVSHFTLADIVAIAQKRHKGTVFSVTP
                     AVIEKHPVAVVLIADKSKVVELDIDMVTGQRVGGDR"
     gene            3820..4707
                     /locus_tag="VE030_11745"
     CDS             3820..4707
                     /locus_tag="VE030_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1259 domain-containing protein"
                     /protein_id="HZF24125.1"
                     /translation="MKTWLKCACLILVVAVALPVTAAELDLARIEALTGLKGKLNEAE
                     GVFKVTFPRTDIKATVTGVRMNPELGLTAWAAFTRAGDHTMVMGDIVLLESEVNPAMS
                     VALDNGLEVTALHNHFLWDNPKVMFMHIGGMGEEEKLAGAVGKVFDKLKAMIRDKPTP
                     PHAQIDPAQTSLDGKRIAEIVGTPGALKDGVYKIVIGRTIDMGGHKVGKEIGVNTWAA
                     FAGSDELTVVDGDIAMLEPEVQGVLKALRAGGINIVAIHNHMTSESPRMVFLHYWGIG
                     PTTQLARTLRAALDTQHLD"
BASE COUNT         1054 a         1463 c         1553 g          881 t
ORIGIN      
        1 acatgctacg ccaccgcaac aacctctgga ccgtcgagtc ctggggagac gtcagccacc
       61 tgccccggga actggcgctg gacgatacgt gagtaaggca aggatgaaga atgaaggcgg
      121 aaggatgaag aaaggcggtg aacgttgatt gcaagagtct gggcgctcct gccccgtcta
      181 cgcagttctt tgcgttctct ctttttctcc tttgcgtact ttgcgtcaac gctcttacgg
      241 tgtcttcggg ggccagccgt gggcttcaga ctggcatgtg cggcaccatg cataaagcgc
      301 atcgtagatc accatcccat gatcgagcat tttgtggtca tccggaaaat tgtgcgacaa
      361 cccgagtgag agggcgagca acccagcggc ctgcggagca agttccaatt gcgcggtgtc
      421 ggcgccgcga acgatgaccg caagctgagc cagtgcggga tcgactaatt ggtatttgga
      481 aagaaaagca tcgaagctgc acagcttgcc gacatgggaa agttctacac cggggatgtc
      541 gtacggaaca gcagcggctg cggcggcgat gcgcagtacg tccccggacg gcacgaacag
      601 gaactcgggt tcaggatcga tgaagcgcgc gatcagccag ggacaggcga tgcggtcgat
      661 cttcgggcgt tcacgcgtga tccatttcat tcgcgcctcc cattgctaga gcaatgttcg
      721 tgcggcctgc actacgagcc cggcagcgcc gcagacggcg atcaccggaa tgatgccggt
      781 cttgtagcgg aacagcgcga ctaccgcgcc gagcgcgatc agcagcgccg gccattccag
      841 gtggccgcgc aggccgtccg gccagaaaac gtgtaccgca aagaacacag caaggttggc
      901 gattacgccg accaccgcgg cggtgatccc ggtcagcggc gccgttagat gcggctggcg
      961 gtgggtggtc tcgatcaccg gcgcaccggc gaagatgaac aggaacgacg gcaggaaggt
     1021 gaaccaggtg gccaccactg ctccggcgat gcccgccaga gccagtgact ctgagccgaa
     1081 tacggagtgt gtccaggcgc caacgaagcc gacgaaggcc accaccatga ttagcgggcc
     1141 aggcgtagtt tcaccgagcg ccaggccgtc gatcatctgc accggggtca gccattggaa
     1201 atgctgcacg gcgccctgta cgacgtaagg caacacggcg taggcgccac cgaatgtaag
     1261 cagcgccgcc ttggtgaaga accagcccat tgcgctcagc gtgttcgccc atccgaacag
     1321 gagcgcgatc gcgcccatcg agacggccca gagcgaaagc ccgaccgcga tcacacgcgc
     1381 gatcccgccc cagcgaaaat gcgcatgcgc gggtgtcggc gtgtggtcgt cgatcagcgc
     1441 cgggccgtag tcccttctgg attcggcatg cgcaccaccg gtggcgaatt tctgtggcgc
     1501 aacgcgccct ccgatcaagc cgatgacgcc ggcggccagt acgatcgcgg gaaatggcat
     1561 attgaagaag ttgatcgcga tgaacgccgc ggcgctgatc gcccacagcg tttcgttcct
     1621 cagcgcgcgt gcgccaatgc gccaggcggc gtagagcacg atcgcagtaa ccgcgggttt
     1681 gattccgtaa agaatgccag ccaccagcgg cgtgcttccc cacaccatgt aaatccacga
     1741 cagcgcgatc aggatcacaa gcgaaggcag gacgaacaac gcccccgcag cgatgcctcc
     1801 cagcgtgccg tgcatcaacc agccgaggta ggtggccagt tgctgcgcct cgggaccagg
     1861 cagcagcatg cagtagttga gcgcgtgcag gaagcggcgt tccgagatcc agcggcggcg
     1921 ctccaccaac tcctggtgca tcaccgcgat ctgcccagcc gggccgccaa agctgataaa
     1981 gccgagcttc agccagtaca gcagggcctc gcgcaacgaa actgcggccg gcgcggcctc
     2041 agcgttgacg gacgaacgtg ccataaagaa agttaagaag cttctctgcc tcgcgcagca
     2101 actcgtcgtc gtccgtgaac gtcgcctgtg cgccacgcag gatggcctca aagccgacgg
     2161 cttcgggcac gggcacacca cctacgtcaa ggtagtgaat cagcgtgccg attttttcca
     2221 cgccaggatc gtgatcgaga tcgaaactcg ttgccagtac ttcgaacgta actctgctac
     2281 cgatatgcgt gaacgtcgca ccgtcgaaat cgaaaccgag cgcgccttcc gggcaatcgc
     2341 ccggcttgtc gagccagaga atgcgcgcct ccggatcgat gaagcgccgg atcagccagg
     2401 cgcttgccag gcgatcggcc cacgggcgct tgcgcgtagc ccaggtgcgg ccacggtact
     2461 cggcaacatc gagtttgcga acgcggccct cgaccgggcg cggttcgtcg ggggagagaa
     2521 cggcgttggc atcggcgagg aaatccttga gagccagtgt ggtttgtgat gccgcgcgtg
     2581 ccgggaagaa atccagagcg acgagatttt cgacttcgcg acgcaaccga gcggctgctg
     2641 tgccgaccgc gccggcatcc atctttggca gcttcttgcg tacgtcgcgg atttctccga
     2701 gcagcttttc gtagtcggtg gtgcggtcga acagcacctc gaattcccgt tgttgttgcg
     2761 cgtcgcgcgc gtcgagttcc aagacctgca cgttgccttc tgcggcggca acctcctgtt
     2821 cgagtgcgcg cagggactcc cggaacgccg gccggttcgg caacaaatag acgccatccc
     2881 gcagaaccgc ggtgccgagc gacttcaatg ctcgccacac ccgcatgcgc aaggcgctct
     2941 gctgcccctg tacggtgagc aggaacagca accaggctgg gcgccgggca tccgttacgg
     3001 agtcctctgt caacgcggtg gtctttgcct cgttcatggt tgtctttaat ataacatata
     3061 tgtagtatat ataactcaat cgagctgaat cgggcttgag cttgactgaa aatatcattt
     3121 aaaacagaag agtagtgaga ttttaagcgt tgccctacca ccaaagattg gatcggaaga
     3181 caacttatca ggagactgga catgaaaatc gatacgcaat tggcgcggtt cgccgtggtc
     3241 gctttactcg cgtccccaat cgtcgtaact gcgccggctg caaccgcaaa ggtctatgac
     3301 gatcaggaaa cagcggcgct ggtgcgagcg ttgtcgggca gcaaggtctc gctgcttgaa
     3361 ggtctgcacc aggtcggcaa ggacgacgaa gcagcgatct cggccaagtt cgagttcgac
     3421 gacaaccaca agctttcgct ctccgtgtat accgcggaga aagggctggc ggtggatgcg
     3481 gaacacaacg tgctgaagga attcagcggc agcccggaag caacagcgtg ggcaccggaa
     3541 gccgaagtgt tcaaggacat ccctcacgtt gcgcgcgcct ctgagcattt ggcgctcatg
     3601 tcggtctcgc atttcacact cgccgacatc gttgcgatag cacagaagcg acataaagga
     3661 acggtgtttt cggttacgcc tgcggtaatc gagaagcacc cggttgcggt ggtcttgatt
     3721 gctgacaaaa gcaaggtcgt cgaactcgac atcgacatgg tcacaggtca gcgcgtgggc
     3781 ggggatcgtt gaatgtttca tccggtcgtg gaggttggca tgaagacatg gctgaagtgt
     3841 gcctgtttga tactggtggt tgcggttgcg ttgccagtga cagcggcgga gctcgacctt
     3901 gccaggatcg aggcgttgac ggggctcaaa ggcaaactca acgaggcgga gggcgtgttc
     3961 aaagtcacgt tcccgcgtac cgacatcaag gcgacagtga cgggcgtgcg catgaacccc
     4021 gagctgggtt tgactgcctg ggctgcattc acccgcgccg gcgatcacac catggtgatg
     4081 ggcgacatcg tgctgctcga atccgaggtc aatccggcga tgagcgtggc actcgacaac
     4141 ggtctcgaag tcaccgcgct ccacaaccat tttctgtggg acaacccgaa agtgatgttc
     4201 atgcacatcg gcgggatggg ggaagaggaa aagctcgcgg gagcagtcgg caaggttttc
     4261 gacaagctga aagccatgat ccgtgacaag ccgacacccc cgcacgcgca gatcgatccc
     4321 gcacagacgt cgctcgacgg caagcgcatc gccgagatcg tcggcacgcc gggcgctctc
     4381 aaggacggcg tgtacaagat cgtgatcggc cgcaccatcg acatgggcgg gcacaaagtc
     4441 ggcaaagaga taggggtcaa tacctgggcc gcttttgccg gcagcgacga actcactgtg
     4501 gtggacggcg atatcgcgat gctcgagccc gaggtgcaag gcgtgctgaa agcgttgcgc
     4561 gccggcggaa ttaacattgt cgcaattcac aatcacatga cctccgaaag cccgcggatg
     4621 gtgttcctgc actactgggg aatcgggccc accacgcagc tagcgcgcac gctgcgcgcc
     4681 gcgctcgaca ctcagcacct ggattgaggt gagcacgaaa cgattctcgg tgctgctggc
     4741 gatggttgcc gctgggtgag cggtcgatcg tcgatgaatc gccggttttt gtctcggctc
     4801 gcggtagcgg aggtctttct cgcaatgagc catagcgtcg cggcccaggg cggccctccg
     4861 cttctcaccg acgatcccga tactccggga aaccggcact gggaaatcaa caccgcctac
     4921 acggtcgaac gggttcgcga cgaagaatcc a
//
LOCUS       DASQOP010000293         4288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2572810, whole genome shotgun sequence.
ACCESSION   DASQOP010000293 DASQOP010000000
VERSION     DASQOP010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2572810"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="VE030_11750"
     CDS             <1..796
                     /locus_tag="VE030_11750"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HZF24126.1"
                     /translation="KDGLVLTIGYLITEARAVWLTTNSGAAVAGYPLAYDQVTGFGLV
                     QPLGRLDAPFLQRGSTASCTAGDDLIVIGHGGRAHALKAKVIAKREFAGYWEYVLDEA
                     LFTAPAHPQWGGAAVVGSDGRLLGIGSLVIEESQSGRKVQGNMAVPIDLLEPILEDLL
                     KFGRTNRPPRPWLGMYTMEANGQLVVAGIADNGPAARAGVRQRDLVLEVAGIRVTALA
                     DLFRKVWSLGPAGTEIPLTLGRDGDLVRARLRSADRNDFLRKPQLH"
     gene            complement(1277..1690)
                     /locus_tag="VE030_11755"
     CDS             complement(1277..1690)
                     /locus_tag="VE030_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012839267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZF24127.1"
                     /translation="MVYKNAYAMTAAALGLALAFGVGVAVGQNAPTENKGVNISPPTA
                     LDLGGEIDGVAGRQLRLRVVTLEPGGVVGVHSHNGRPGVAYVLQGTLTEHVDGSGMHE
                     RSEGESWTEGKAITHWAENKGRKPVVVIAVDVFKP"
     gene            1877..2503
                     /locus_tag="VE030_11760"
     CDS             1877..2503
                     /locus_tag="VE030_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24128.1"
                     /translation="MTNAFTFFRSLLADPRRVAAIAPSSPALANAITAEITPVSAPVI
                     ELGPGTGVFTRALLARGVPEDKLALVEYGPDFARMLQSRYPRAYVLRMDAARLKSVEL
                     FGGERVGAVVSGLPLLSMPAHLVSAILDGAFAHMRADGAFYQFTYGPRCPVRRSILER
                     FGLKAVRIGGALANMPPASVYRIRRRPPVRASATGMLAHVHGGDGDTF"
     gene            complement(2690..3250)
                     /locus_tag="VE030_11765"
     CDS             complement(2690..3250)
                     /locus_tag="VE030_11765"
                     /inference="COORDINATES: protein motif:HMM:NF019128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HZF24129.1"
                     /translation="MRRTARRVPLLSSWRNDARFFWHTRNKAPWIATLIVAAAITTFS
                     AIMYWLESNSSEKRELTCLALNVYYEARGEPLAGKYAVAEVTMNRVSSRRYPGTVCEV
                     VYEKRWDWLRKRYVGAFSWTEFDIVPHPEGEQWHQAREVAEAVYSGRQPPQLNGALHY
                     HATYIRPSWARGRQPVARIGGHVFYK"
     gene            3699..>4288
                     /locus_tag="VE030_11770"
     CDS             3699..>4288
                     /locus_tag="VE030_11770"
                     /inference="COORDINATES: protein motif:HMM:NF021472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HZF24130.1"
                     /translation="MDKPINAFAHSPGLPLVARVGALQPLHWLARGWEDFTANPGPSV
                     VHGLILVALGWLILLMCSTQIDLLAAAVSGFLLVGPVFGAGFYELSRLRASGQRATFD
                     ASLDGALRNGRSLARLGLLLAILAVAWVLISRLLFVQTLGGTLPSVRETFYQTIIDWD
                     YYEFFVTYMSTGVVFAVVAFIVSAVSAPMIFDRAADT"
BASE COUNT          741 a         1390 c         1299 g          858 t
ORIGIN      
        1 caaggacggg ctggtgctca ccatcggcta tctcatcacc gaggcccgcg cggtatggct
       61 gacgaccaac agcggcgcgg cggtcgcggg atatccgctg gcctatgacc aggtaaccgg
      121 tttcggtttg gtgcagccgc tcggccggct cgatgccccg ttcctgcagc gcggttcgac
      181 cgcctcgtgc acggccggtg acgacctgat cgtgatcggc cacggcgggc gggcgcacgc
      241 gctgaaggca aaagtcatcg ccaaacgcga gttcgccggt tactgggagt acgtgctcga
      301 tgaagcgttg ttcactgcgc cggcgcaccc gcaatggggc ggcgcggcgg tggtcggcag
      361 cgacggacgg ctgctcggca tcggttcgct ggtgatcgag gaaagccaaa gcggccgcaa
      421 ggtacagggc aacatggccg tgccgatcga tctgctggaa ccgatactcg aggacctgct
      481 caaattcggc cgcactaacc ggccgccgcg tccgtggctg ggaatgtaca cgatggaagc
      541 caacgggcag ctcgtggtcg ccgggattgc agacaacggt ccggcggccc gtgccggcgt
      601 gcgccagcgc gatctggttc tcgaagtcgc gggtatccga gtcaccgcgc tcgccgatct
      661 gtttcgcaag gtgtggagcc tggggcccgc gggcaccgaa attccgctca cgctggggcg
      721 cgacggtgat ctggtgcgtg cgcggctgcg ctccgccgat cgcaacgatt tcctcagaaa
      781 accgcaattg cactgacagg cacccaccgc tcggcgacag gcgcttcgag tgctccgggt
      841 gagctgtggc gcctgtcccg gccgcggcgt aaaattctgc tcatgcgctg cccatactgc
      901 cagagccaga acgtcgcacc ggcccccgaa gccatccaaa gccatgcgct gcagcaccct
      961 gactattatc agtgcggcga ctgcggcaaa cgaatttcag tgacaaaagc gccccggccg
     1021 ggtgccgcaa accggcccgc cccgccgatg ttcaaagcgc aggttttcag gcgggcacgg
     1081 ggtaatccgc gcggtgcgcg cggacgatag cggtgaaaac tttcgcatcg acatacgccc
     1141 gcatcgtgag cccgaccgat tcgcgctcgg cgaagacgcc tcaccgacgt aacgtcaaaa
     1201 tttaagagat attggacccg ggactcagtg aacggccaac atccttgctt ttccccgcca
     1261 ctcgtcaccg cagtctttac ggcttgaata catcaacggc gatgaccacg accggcttgc
     1321 ggcccttgtt ctcggcccaa tgggtgatgg ccttgccttc ggtccagctt tcaccctcgc
     1381 tgcgttcgtg catgccgctg ccgtcgacgt gctcggtgag cgtgccttgc aaaacgtagg
     1441 ccacaccggg ccggccgttg tgactatgga cgccgaccac accgccgggt tccagcgtaa
     1501 ctacccgcag ccgcagttgc cggcctgcca ccccgtcaat ctcccctccc aggtcgagcg
     1561 cggtaggcgg gctgatgttc acccccttgt tttcggtcgg cgcattctga ccgaccgcaa
     1621 cccccactcc gaaagccagc gccaacccca aggccgctgc cgtcattgca tacgcgttct
     1681 tataaaccat tgcgaacctc ctccttgaca gtcgtcggca atgctccccc gacagcgatg
     1741 aatcctaccc ctcctgccgg cgtgcgtcag ctgcgaaccc gaagccgggc ccgcaattct
     1801 tttgctcgcg caccgccccc gatttgaagt acattgcgaa cgcgatcctt tttgcctgcg
     1861 gacaacccga caataaatga ccaatgcctt cacgttcttc cgctctttgc tcgccgatcc
     1921 acggcgcgtt gcggctatcg cgccgtcgag cccggcgctg gccaacgcca ttaccgcgga
     1981 gatcacaccg gtctcggccc cggtcatcga actcgggccc ggcaccgggg tattcaccag
     2041 agcgctgctt gcgcgaggtg tccccgaaga caagctggcg ctggtcgagt acgggcccga
     2101 cttcgcgcgc atgctccagt cccgctaccc gcgtgcatac gtcctgcgca tggacgcggc
     2161 gcgcctgaag tcggtcgagc tgttcggagg cgagcgcgtc ggcgcggtgg tgagcgggct
     2221 gccgctgctg tcgatgcctg cacatctggt atccgccatc ctcgacggcg cattcgcgca
     2281 catgcgcgcc gacggtgcgt tttaccagtt cacttacggc ccgcgctgcc ccgtgcgccg
     2341 ctccattctc gaacggttcg gactcaaggc ggtccgtatc ggcggcgcgc tggccaacat
     2401 gccgcctgca tctgtctacc gcatacgccg ccggccgccg gtgcgtgcct cggcgactgg
     2461 aatgctcgca catgttcacg gcggcgacgg cgacaccttt taacgacgct acgctggcgg
     2521 acagagcggt tactggtgac tagtgacgag gggctagagg ctagaaaatt ctagtcccta
     2581 cgatttgtgt ttcctaagcg ttcttcacgt caacgctctt ttagccggcg ataggccggc
     2641 tgcttgtcct gaatcccgag tcctgtttac caccgattcc cgcggccaat tacttgtaga
     2701 aaacatgccc gccgatgcgt gcgacgggtt gccggccgcg cgcccagctg ggtctgatgt
     2761 aagtcgcgtg ataatgcaaa gcgccgttca gttgcggcgg ctgccttccg gagtagacgg
     2821 cttcggcaac ttccctggcc tgatgccatt gctcgccttc cgggtgcgga acaatgtcga
     2881 actccgtcca tgagaatgcg cccacgtaac gtttgcgcaa ccagtcccat cttttctcgt
     2941 agacgacttc gcagaccgtt cccgggtaac ggcgcgaaga tacgcggttc attgtgactt
     3001 ccgccacggc gtatttcccc gcaagtggct ctccccgggc ttcgtagtac acgttcagcg
     3061 caagacacgt cagttcgcgc ttctccgaac tgttcgattc gagccaatac atgattgcac
     3121 tgaacgtcgt gatggctgcc gcgacaatca gggtggcgat ccatggagcc ttgtttctgg
     3181 tgtgccagaa aaagcgcgca tcgttccgcc agctagacag cagcggaaca cggcgcgcgg
     3241 tacgtcgcat gtagaagagt tgtcgcaata ttttccgggg tcgatggctg gcgcgctgcg
     3301 tcacatcttc gcaccatgta gtgccattgc agttttatga cgcaagattc gggcctttcc
     3361 tgacagcctt ttgccggaca gcgggtgccg atattcagtg caaaacggca ggtccttgca
     3421 gaattcgaac gacaattacg cgcaggatcg gagaaactta tgcagattct tctgccttca
     3481 ccggcaggga catcgagtgc cgcgcaatga cctatacagc aaggaacagg ccaacgttgt
     3541 cttccatttg ctcctgatgc cgcactgcgg gcaaccgccg ccgccgaaaa tggaggaaac
     3601 tgttttgcaa gtgcgccaac ctgcggcacc ggccctggac atggccgcgg gcagcttcgc
     3661 agtagacgtc gcagcttcat cgtaaggagg ggcgaatcat ggacaagcct atcaatgcat
     3721 ttgctcactc tccgggtttg ccgctggtgg cccgcgtcgg cgcgctgcaa cccctgcact
     3781 ggctggcgcg cggatgggag gatttcaccg ccaaccccgg gcccagcgtc gtccacggat
     3841 tgattctggt cgcgctgggc tggttgatct tgttgatgtg cagcacgcag attgatctgc
     3901 tcgccgcggc ggtgtccggc ttcctgctgg tggggccggt attcggtgcc ggattttacg
     3961 agctgtcgcg gctgcgcgcc tccggccagc gcgccacctt cgacgcgtcg ctcgacggtg
     4021 cgctgagaaa cggtaggtcg cttgcgcgcc tggggttgct gctcgcgatc ctggcggtcg
     4081 catgggtatt gatatcgagg ttgctgttcg tgcagacctt gggcggcacc ctgccgtcgg
     4141 ttcgcgaaac cttctaccag accatcatcg actgggacta ttacgagttc ttcgtcacct
     4201 acatgagtac cggtgtggta ttcgcggtgg ttgcatttat cgtctccgcg gtctcggcgc
     4261 cgatgatctt cgaccgtgcg gccgacac
//
LOCUS       DASQOP010000294         2065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2969455, whole genome shotgun sequence.
ACCESSION   DASQOP010000294 DASQOP010000000
VERSION     DASQOP010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2969455"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="VE030_11775"
     CDS             complement(<1..870)
                     /locus_tag="VE030_11775"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HZF24131.1"
                     /translation="MRPRPDFFIAGALKCGTTSLFIYLRAHPAVFMPANKEPNYFCGD
                     LVAHWPDGASFRVGTLAEYEALFSTAPPQALTGEASVFYLYSKLAIGQIMARNVNAKI
                     IVMLRNPIEAARSLHAYHWNNHLEDVRDFEQAWRLQEPRLEGRHLPPRWPYPQLLQYG
                     AQYSYAPQVRRLLAQVPRKQCLFLLFEEFFADPSRQFARVLDFLGLPPDPARTAFPVV
                     NQTIGVRSLRLDRLLRQPPPALLALRHTAHAFGFHPVRALKLLNRVAGERPPLREAFR
                     AELEEYFSVDVAEL"
     gene            complement(884..1894)
                     /locus_tag="VE030_11780"
     CDS             complement(884..1894)
                     /locus_tag="VE030_11780"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZF24132.1"
                     /translation="MEFVAAVGLVGLALVLDGREDGVGPWLGQLTFLAFAAYRLLPIL
                     QQVFAASVNIRADRAGLALIAPDLRRARAAVHTTTPANFTLTDCARQVRPQREIRLEE
                     VSFRYAPDQPWALMGVSLRIPAGAAVGIVGVNGSGKTTLVDVIVGLLVPSEGRVEVDG
                     SALDEANRAAWQSRIAYVPQNIFLLDSSIAENIALGIPAADINRHRLSEAVRLAQLDE
                     FIKTLPAGCDHRVGERGIQLSGGQRQRIGIARALYRAATVLVFDEATNALDGLTEQEL
                     MSTLGRLRGRYTTILIAHRMTTVRSCDIIFELENGKITGSGTYDGLLKSSAVFRRMTG
                     VR"
BASE COUNT          422 a          631 c          639 g          373 t
ORIGIN      
        1 tagttccgcg acatcgacgg aaaaatattc ctcgagctcc gcgcgaaacg cctctcgcag
       61 gggcggcctc tcgcccgcga cccggttgag gagcttcagt gcacgcacgg ggtgaaaacc
      121 aaacgcatgt gccgtgtgcc gcagggccag gagtgccggc ggtggctgac gcagcagtcg
      181 atccaggcgg agggatctca ccccgatcgt ctggttcact accgggaaag ccgtcctggc
      241 ggggtcgggc ggcaaaccca gaaaatcgag cacccgcgcg aactggcgcg atggatcggc
      301 aaagaattcc tcgaagagca agaacagaca ctgctttcgc ggcacctgcg ccaacagccg
      361 acgcacttgc ggcgcatagg aatattgggc tccgtactgc aacagctgag ggtacggcca
      421 gcgcggcggc aagtgccgtc cctcaagacg cggctcctgc agtcgccacg cctgctcgaa
      481 atcgcggacg tcttccaggt ggttgttcca gtggtaggcg tgcagagagc gcgccgcctc
      541 gatggggtta cgcaacatta cgatgatctt ggcattgacg ttgcgcgcca ttatttgacc
      601 gatcgcgagc ttcgaataga ggtaaaacac cgatgcctcg cccgtcagcg cctgcggcgg
      661 agcggtcgag aagagcgcct cgtactccgc gagggtgccg acgcggaagg acgctccgtc
      721 gggccagtga gctaccaggt cgccgcaaaa gtagtttggc tccttgtttg cgggcatgaa
      781 caccgctgga tgcgccctga ggtagataaa cagcgaggtg gttccgcatt tgagggcacc
      841 ggcgatgaag aaatctggcc taggtctcac gccatgcctc cactcagcgg accccggtca
      901 ttcgccggaa cactgccgaa ctcttcagga gtccgtcata agtaccgctg ccagtgattt
      961 ttccgttttc gagctcgaag atgatgtcgc atgagcgcac cgtggtcatt cggtgcgcga
     1021 tgagaatagt cgtatagcgg ccgcgcaaac ggccaagcgt cgacattagt tcctgttccg
     1081 tcagaccgtc caaagcattc gtagcctcgt cgaacacaag gacggttgct gcccgatata
     1141 gcgcgcgcgc aatgccgatt ctctgccgct ggccgccgct cagctggatg cctcgctcgc
     1201 ccactctatg atcgcagcct gcggggaggg tcttgatgaa ctcatcgagt tgcgccaggc
     1261 gaaccgcttc cgacagccgg tgtcgattga tgtccgcggc aggaattccc aacgcaatgt
     1321 tctccgcgat gctcgagtcc agtaggaata tattttgcgg aacataggca attcgggact
     1381 gccaggccgc acggttggcc tcgtcgagcg cgctcccatc cacttccacc cgtccctcag
     1441 aaggcactag cagccccacg atcacatcca ccagtgtcgt ctttcccgag ccgttgacgc
     1501 cgacgatgcc taccgcagca ccggcgggga ttcgcaggga gacccccatg agggcccaag
     1561 gttggtcagg ggcataacga aacgacactt cttcgagccg gatctcccgt tgagggcgca
     1621 cctgccgtgc gcaatccgtc agcgtgaagt ttgccggagt ggtcgtgtga actgcagctc
     1681 gagctcgccg caaatccggc gcaatcaggg cgagcccggc gcggtcggcg cgaatattca
     1741 cggacgcggc aaatacctgc tgcaggatcg gcaagagccg gtaagcggca aatgcgagaa
     1801 acgtcaattg tcccagccag gggccgacgc catcctcgcg accgtcgagc acgagcgcga
     1861 ggccgaccag gccaaccgcg gcaacgaatt ccatgaggtg cctgggattt tgtgccgccg
     1921 cttgactgtg agcggcggcg agagaaaagg acttgctggc tcgctcaaat cggccgcgaa
     1981 agaaattctg cgcctgcaga aggatgattt ccttgatggc gccgaagctc tcgtgcagta
     2041 tctgctcctg ttcgaaggca gagcg
//
LOCUS       DASQOP010000295         2137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_351147, whole genome shotgun sequence.
ACCESSION   DASQOP010000295 DASQOP010000000
VERSION     DASQOP010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351147"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..1798)
                     /gene="phaC"
                     /locus_tag="VE030_11785"
     CDS             complement(68..1798)
                     /gene="phaC"
                     /locus_tag="VE030_11785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HZF24133.1"
                     /translation="MSSPAESSPGAYQELAKANQALVEDFLRQLPSSGRLEFTELMRT
                     LAFGSDAQREKLRSLQERLYRAHLALWGSLLGGGNDTVQDADAGGDHRFDAPEWSEVP
                     FFRYLRQAYLVNARFFDELGELAELDGHAKRRLQFVLRQFADAIAPTNFAATNPEVIK
                     LAGESRGESLSQGMKLLAADLARGRISMTDERAFEVGRNLAVTPGAVVLENEVMQLIQ
                     YAPSTDEVFERPLLIVPPFINKYYILDLQPENSFASYCVEQGFTTFIVSWRNIPPELG
                     HLTWDDYVREGVLDPLDAVRTITRAGQVNTLGFCVGGTLLATALAVMAQKNDASVASL
                     TLLASMLDFSETGDISVFVDREFVERAEQEFRSSGSMPGSRLAATFATLRANDLVWHF
                     VINNYLKGREPKPFDLLYWNADGANLPGRLYAYYLRNMYLENNLRIPGKLESCGVPVD
                     LRRIEVPAFVLATREDHIVPWKTAHLSAALLGGCSEFVLGASGHVAGIVNPAAKNRRH
                     YWTNSTAAPDADAWFAGAVQQTGSWWPHWVNWLRENSGNKQLAPPALGNTRFSVIEPA
                     PGRYVKEKLR"
     gene            complement(2036..>2137)
                     /locus_tag="VE030_11790"
     CDS             complement(2036..>2137)
                     /locus_tag="VE030_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004544445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HZF24134.1"
                     /translation="GGGLCTYSDPRRFFSHRRDKVTGRMAALIWLAD"
BASE COUNT          408 a          653 c          669 g          407 t
ORIGIN      
        1 cgtttcgttt tgccatggct ggttcctccc aatttcttgg ttgaactccg ccgccgggtg
       61 tatgccctca gcggagcttt tccttcacgt accgtccggg cgccggctcg atcacggaaa
      121 atctcgtgtt ccccagtgca gggggtgcaa gttgtttgtt gcccgagttc tcgcgtagcc
      181 agttcaccca gtgcggccac cagcttccgg tttgttggac tgcaccggca aaccaggcgt
      241 ccgcgtccgg cgccgccgtg ctattggtcc aatagtggcg gcggttcttc gctgcggggt
      301 tgacgatgcc cgccacgtgt ccgctcgcgc cgagcacaaa ctcgctgcac ccgcccagca
      361 atgccgcgct caggtgcgcg gtcttccagg ggacgatatg atcttcgcgg gtggccagca
      421 caaaggccgg cacctcgatt ctgcgcaggt ccacaggcac gccgcacgat tcgagtttac
      481 cgggaattct cagattgttc tccaggtaca tgttgcgcag gtaataggcg tatagacggc
      541 ccggcaggtt ggctccgtct gcgttccagt agagcaggtc gaagggcttc ggttccctgc
      601 ctttcagata gttgttgatc acgaaatgcc acaccagatc gttcgcgcgc agcgtcgcaa
      661 aggttgcggc gagccggctt ccgggcatcg agccgctgct gcgaaattcc tgctcggcgc
      721 gctcgacgaa ttcccggtcc acgaatacgc tgatgtcgcc agtctcggaa aaatccagca
      781 tcgaggcgag caatgtgagc gaggcaaccg aagcatcgtt cttctgcgcc atgactgcga
      841 gcgcggtcgc aagcagcgtg ccgccgacgc agaaacccag cgtgttgacc tgtccggcgc
      901 gggtgatcgt gcgcaccgca tcgagcgggt ccagtactcc ttcgcggacg tagtcgtccc
      961 aggtgagatg tccgagttcc ggcgggatgt tgcgccagga gacgatgaaa gtcgtgaagc
     1021 cctgctcgac gcagtagctc gcgaaggaat tttccggttg caggtcgagg atgtagtact
     1081 tgtttatgaa cggcggcacg atgagcaggg gccgctcgaa aacctcgtcg gtagagggag
     1141 cgtactggat cagctgcatc acttcgttct ccagcactac cgctccgggc gtgactgcga
     1201 gattacggcc gacttcgaag gcacgctcgt cggtcatcga aatgcggccg cgcgcgagat
     1261 ccgcggcgag caacttcatc ccctgcgaca ggctttcgcc gcggctctcc ccggcgagct
     1321 tgatgacttc aggattggtc gcggcaaaat tggtgggcgc gatcgcgtcc gcgaactggc
     1381 gcagcacgaa ttgcagccgc cgcttcgcgt gaccgtcgag ttccgccagt tcgcccagtt
     1441 cgtcgaagaa acgcgcgttc acaaggtaag cctgccgcag gtaacggaag aacggcacct
     1501 cgctccattc cggcgcatcg aaacggtgat cgccgccagc gtcggcatcc tgcacggtat
     1561 cgttgccgcc accgagcaga ctgccccaga gcgcgagatg cgcacgatag aggcgttcct
     1621 ggagggagcg caatttctcg cgctgtgcat ccgaaccgaa tgccagcgtg cgcatcagtt
     1681 cggtgaattc gaggcggccg gatgagggaa gctggcgcag aaaatcttcg accagggcct
     1741 gattggcttt ggcgagctcc tgataggcgc cgggagagga ttctgcaggt gaactcatgt
     1801 cccggtggac gccggaagaa tcgaaatcac aacacgttcg ggattcttga ccgcacccct
     1861 ggtcaagtca agctgcacac gccgcttaat cctagccatg accccttgca aggaagccaa
     1921 ccagcgcgat cgagccgatg cccagcgaaa tgagcgacac ctgctcgagc gcgtgtggca
     1981 ggatttcgag gaacaccgcg ccgagcagcg caccggtggc gtagctgaca aaccctcagt
     2041 cggccagcca gatcagggcg gccatgcgac cagtgacttt gtctcgccga tgggagaaga
     2101 aacgccgcgg gtcggagtaa gtacagaggc cgccacc
//
LOCUS       DASQOP010000296         4652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2169282, whole genome shotgun sequence.
ACCESSION   DASQOP010000296 DASQOP010000000
VERSION     DASQOP010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2169282"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1410)
                     /locus_tag="VE030_11795"
     CDS             complement(139..1410)
                     /locus_tag="VE030_11795"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZF24135.1"
                     /translation="MNATLFRNVRVLDCTGNPAFAGSVLVEGDRIAAVVPAAASLAFG
                     NARVVDGQGATLMPGLIEAHSHLSFTDFAQSVELGFIPPEEHTLRTAQNARRMLDAGF
                     TSCFSAASAKPRLDIVLRNAIDNGDFPGPRTLAASPEMTVTSGLGDVRLYHMYRENFG
                     VVCNGAEEFRRFSREMVREGADTLKINVSGDDGVPASRADTTVMTETEVAAVCDVARA
                     HGKRVAAHARSAESVKMCLRHGVEVIYHATLLDEEATDRLEAQKDRIFVAPVLGHLYT
                     TLNEAGPWGITREVAEKRGTGRELERGIESMKDLKRRGVRILIGGDYGFAWNPVGTDA
                     RDIAHFVNLLGFTPMDAIIAATKLGGEIMGMGNRLGQIKAGFLADLLLVNGDPLEDVA
                     ILQDKSRIPGIMKNGVFWRAPDPALVGSARH"
     gene            complement(1427..2176)
                     /locus_tag="VE030_11800"
     CDS             complement(1427..2176)
                     /locus_tag="VE030_11800"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZF24136.1"
                     /translation="MISLTGKVAWITGAGTGIGLAGAKALADAGATVVMSGRRKEVLA
                     LEAEKIRIKGGKSEIEALDVSEAKAVKKTASAILSRHARVDILVNSAGLNVPNRYWKN
                     QTVEGWKEVIGINLDGSFYTTLAVLPSMRARKDGLVINVSSWAGIFHPKLTGAAYNAS
                     KHAVTAMTETINMEECANGIRACAICPAEVATPIMDRRPVPPSQQDRARMLQPEDLGN
                     TIRFIAQMPPHVCINQVIISPTWNRMYIENL"
     gene            complement(2188..3168)
                     /locus_tag="VE030_11805"
     CDS             complement(2188..3168)
                     /locus_tag="VE030_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004539804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZF24137.1"
                     /translation="MALDPQIEFVIGLVKKANAPEFWQLTPDQARSQYLLRVAKLAVK
                     EPIFRTEDRRISGPGSHIPIRIYTPREIKPGEKLPVLLWFHGGGFVIGSLETHDSVCR
                     MLANQADCIVVSADYRLAPEYKFPAAVEDCEAALKWVALHAVEFGADPQAIAIGGDSA
                     GANLATVVAILARDAGHPKLAFQLLIYPCAAPEPETPSHYKFAEGYVLSRNTITWFFK
                     HYQRSRADSNDFRFAPLAADDLANLPPALVLVAGYDPLRDEGVDYAKRLIEAGNRVRL
                     VNYEGMIHGFILMGGAVDAAKRAVAESAQALREAFAGTRTVGKKDSAFAK"
     gene            complement(3434..3850)
                     /locus_tag="VE030_11810"
     CDS             complement(3434..3850)
                     /locus_tag="VE030_11810"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZF24138.1"
                     /translation="MAKVATGFDIHVPFVEHLGLQLLEKRNGVVRMRLDPRPELANSW
                     GSVHGGALMTLLDVALASAGRSLDEHCNGALTVEIKVNFIAAAGGPVHAEARAQRAGR
                     SLIFSEGELRSEDGSLLAKATGTFKLLYPSPKDQGR"
     gene            complement(3855..4631)
                     /locus_tag="VE030_11815"
     CDS             complement(3855..4631)
                     /locus_tag="VE030_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZF24139.1"
                     /translation="MSVKKLLDLTGKTALITGGSRGLGLQIAEALGEMGAKVAITARK
                     AGELAEAKAHLKGLGVDALTVVNDLQDTASVQPMVEQVLKSHGQIDILVNNAGAAWGA
                     PAEDHPLDAWYKVINLNLTAVFLVSQAVAKLSMIPRKYGRIINLASIAGLVGTDPRMM
                     PTIAYNASKGGVVNFTRSLAVEWAHYGITVNAIAPGVFPSKMSQGMIDKAEQVILEIT
                     PMKRLGADYDLKGLAVLLASDASAYITGQIVAVDGGLVAM"
BASE COUNT          800 a         1524 c         1388 g          940 t
ORIGIN      
        1 tcgccacgct tccatatcga cgaatcggga ctgctgcagg gcgcacgctc gctggccgcg
       61 ttggcggtgg atttcctctc cagccgctag tccttcaacc gcgaacgcct tgattgccgt
      121 gcgcccctga ttccagactc agtgtcgcgc tgatcctacc agggctggat cgggcgccct
      181 ccagaacacc ccgtttttca tgattcccgg gatcctgctc ttgtcctgca gaatggcgac
      241 gtcttcgagc ggatcgccgt tgacgagcaa caggtcggcc agaaagcccg ccttgatctg
      301 acccaggcgg ttgcccatgc ccatgatctc gccgccgagt ttcgtcgccg cgataatcgc
      361 gtccatgggc gtgaacccaa gcagattcac gaagtgcgca atgtcgcgcg catcggtgcc
      421 gaccgggttc caggcgaacc catagtcgcc accgatcagg atgcgtacgc cgcgccgctt
      481 caggtctttc atactctcga tcccgcgttc gagttcccgg ccggtcccgc gcttctccgc
      541 aacttcgcgg gtaatccccc acggccccgc ttcattcagc gtggtataga gatggccgag
      601 caccggcgcc acgaaaatcc ggtctttctg cgcttccaac cggtccgtcg cttcctcgtc
      661 gaggagcgtg gcgtgataaa tgacttccac tccgtgccgc aggcacatct tcaccgattc
      721 ggcgctgcgg gcgtgcgccg ccacgcgctt gccatgtgcg cgggccacgt cgcaaacggc
      781 agcgacctcg gtctcggtca tcaccgtcgt atcggcgcgc gatgcgggca ctccgtcatc
      841 ccccgacacg tttatcttca gggtatccgc accctcgcgc accatctccc gcgagaagcg
      901 ccgaaactct tccgcgccgt tgcagaccac gccgaagttc tcccgataca tgtgataaag
      961 acgcacatcg ccaagacccg atgtgacggt catttccgga ctggccgcca gcgttctcgg
     1021 accgggaaaa tcgccgttgt cgatggcgtt gcgaagcaca atgtccagcc tgggtttggc
     1081 cgacgcagcg ctgaagcaac tggtaaagcc ggcgtcgagc atgcgccgcg cgttctgcgc
     1141 ggtgcgcaat gtatgctctt cgggcggaat gaatccgagc tcgaccgact gggcgaagtc
     1201 ggtgaacgaa agatgcgaat gcgcctcgat gagcccgggc atcagcgttg cgccctggcc
     1261 gtcgacaacc cttgcgttgc cgaaagcaag cgaggcggcg gccggaacga ccgccgctat
     1321 cctgtcgcct tcgacgagca cagaaccggc gaacgcgggg ttgccggtac agtccagaac
     1381 tcttacgttc ctgaagagtg tcgcgttcat ggtttttcgg caacaactag aggttctcga
     1441 tatacatcct attccaggtc gggctgatga tcacctggtt gatgcacacg tgcggcggca
     1501 tctgggcaat gaaacggatg gtgttgccaa ggtcttcggg ctggagcatg cgcgcgcggt
     1561 cttgctggct cggcgggacc gggcgccggt ccatgatcgg cgtggccact tccgccgggc
     1621 agattgcgca agcgcggatg ccgttcgcgc actcctccat gttgatggtc tcggtcattg
     1681 ccgtgacggc gtgtttgctg gcgttgtagg cagcgccggt cagcttcgga tggaagatgc
     1741 cggcccagga ggaaacgttg atgaccaggc catctttcct ggcgcgcatc gacggcagca
     1801 ccgcaagggt ggtgtagaac gagccgtcga ggttgatgcc gatcacttcc ttccagccct
     1861 cgacggtctg gttcttccag tagcggttcg gcacgttcaa tcccgcgctg ttgacgagga
     1921 tgtccacgcg tgcgtgacgg ctgaggatgg cgcttgccgt tttcttaacg gctttcgctt
     1981 ccgagacatc cagcgcctcg atctcggact tgccgccctt gatgcggatc ttttccgcct
     2041 ccagcgccag cacttccttg cggcgtcccg acatgaccac cgtcgccccc gcatccgcca
     2101 gcgccttggc gccggccagg ccgatccccg ttcccgcgcc tgtaatccag gccactttcc
     2161 ccgtcagcga tatcattgcg cctcctgtta ttttgcgaac gccgagtcct tcttccccac
     2221 tgtgcgcgtg cctgcaaacg cttcgcgcag cgcttgcgcc gattccgcca cggcgcgctt
     2281 cgccgcgtcc actgcgccgc ccatcaggat gaagccgtgg atcatgccct cgtaattgac
     2341 gagccgtacg cggttgccgg cctcgatcag ccgcttcgcg taatccacgc cctcgtcgcg
     2401 cagcggatcg tagcctgcca ccagcaccag cgccggcggc agattcgcga ggtcgtcggc
     2461 ggccagcggc gcgaagcgga aatcgttcga gtccgcccgg ctgcgctggt aatgcttgaa
     2521 gaaccaggtg atggtgttgc ggctcagcac atagccctcg gcaaatttgt agtgcgaagg
     2581 cgtctccggt tccggcgctg cgcagggata gatcagcaac tggaacgcga gcttcggatg
     2641 gccggcatcg cgtgccagga tggccaccac cgtcgccagg ttcgccccgg cgctgtcccc
     2701 gccgatcgcg atggcttgcg gatccgcgcc gaactccacc gcgtgcagcg ccacccactt
     2761 caacgccgcc tcgcaatcct ccaccgccgc gggaaacttg tactcggggg cgagccggta
     2821 gtccgccgag actacgatgc agtcggcctg gttggccagc atccggcaga cggaatcgtg
     2881 ggtttcgagc gagccgatga cgaagccgcc gccgtggaac cacagcagca ccggcagctt
     2941 ttcaccgggc ttgatctcgc gcggcgtgta gatgcggatc gggatatggc tgccgggacc
     3001 ggaaatgcgc cggtcctcgg tcctgaaaat cggttccttg accgccagct tggcgacgcg
     3061 cagcaggtat tggctgcgcg cctggtcggg cgtgagctgc cagaactcgg gggcattggc
     3121 cttctttacc aggccgatga cgaattcgat ctgcggatca agcgccatgc attctctctc
     3181 tttctctagg aacagctccc accacggagc acacggagga cgcggaggac gcggaggaaa
     3241 aacaaaaacg aggaaaagaa aattggagga aaaagcgagg taactgttcg ctaacctgtg
     3301 tggccaacgt tcgctgcaat aaatcgtgag ccaacgaaat atttttaccc caacttgttt
     3361 tcttgctctt gcctttcctc cgcgtcctcc gtgtcctccg tggttagcgc ccgttttatt
     3421 cagattacat cgtctaccgt ccctggtcct tcggggacgg gtacagcagt ttgaaggtgc
     3481 cggtcgcctt tgccagcaag ctaccatcct cgctgcgcag ttcgccctcg gaaaagatca
     3541 gcgaacggcc ggcgcgctgg gcgcgggcct cggcgtgcac cggcccgccc gcggcggcaa
     3601 tgaagtttac tttgatctct acggtcagtg cgccgttgca gtgctcgtcg agcgagcgtc
     3661 ccgcgctcgc cagagccacg tcgagaagcg tcatgagtgc gccgccgtgt accgaacccc
     3721 agctgttcgc aagctccggc cggggatcga ggcgcatgcg caccacaccg ttacgcttct
     3781 ctagaagctg cagaccaagg tgctccacga agggcacatg gatgtcgaag cctgtcgcca
     3841 cctttgccat ggcgttacat cgcgaccagt ccgccgtcga cagcgacgat ctgcccggta
     3901 atgtaagcgg acgcatcgga cgccagcaac accgccaaac ccttcagatc gtaatccgca
     3961 cccagccttt tcatcggagt aatttccagg atgacctgct cggccttgtc gatcatgccc
     4021 tgcgacatct tcgacggaaa tacgcccgga gcgatggcgt tcaccgtgat cccgtagtgc
     4081 gcccattcca ccgccaagga gcgcgtgaaa ttgaccacgc cgcctttgct cgcgttgtag
     4141 gcgatggttg gcatcatgcg tggatcggtg cctaccagtc cggcgatgga ggcgaggttg
     4201 atgatccgcc cgtacttgcg cggaatcatg ctgagtttcg caacagcctg gctcaccagg
     4261 aacaccgcgg tcagattcag gttgatcacc ttgtaccagg cgtccagcgg atgatcctcg
     4321 gccggcgcgc cccaggctgc gccggcgttg ttcaccagaa tgtcgatctg gccgtggctc
     4381 ttcagcacct gttcgaccat cggctgcacc gatgcggtgt cctgcaggtc attcacgacg
     4441 gtcagcgcgt caacgccaag gcctttgaga tgcgccttcg cctccgccag ctcgcctgcc
     4501 ttgcgggcgg tgatcgccac ttttgcaccc atctcgccga gggcttcggc gatttgcagc
     4561 ccgagtccgc gcgagccgcc ggtgatcagc gctgtttttc cggtgaggtc cagtagcttt
     4621 tttacgctca tggtctgttc cgttgcttat gg
//
LOCUS       DASQOP010000297         4581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_556756, whole genome shotgun sequence.
ACCESSION   DASQOP010000297 DASQOP010000000
VERSION     DASQOP010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556756"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VE030_11820"
     CDS             <1..615
                     /locus_tag="VE030_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24140.1"
                     /translation="AKYWGAIANHSGDAYFDFVYWHDWPNTLNELAKHRVPKRKAGAY
                     DALRESSRKGLAEGLDDGRIKRFLAHVWKKEKLTTAESHCIMNLCMAATYDPDPRAPL
                     GFRVPFNLESGEVIEKRWSKWREHDPIHLVAKYRDNLKSLRGIYIDCGWRDQYHIHYG
                     ARILSKRLSQAGIRHSCEEFDDDHSDIDYRMDVSLPFLYRALKP"
     gene            899..2563
                     /gene="ettA"
                     /locus_tag="VE030_11825"
     CDS             899..2563
                     /gene="ettA"
                     /locus_tag="VE030_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HZF24141.1"
                     /translation="MAQYVYTMQRVGKIVPPKRQILKDISLSFFPGAKIGVLGLNGSG
                     KSSLLKIMAGLDQEIEGEAIPMPDLRIGFLPQEPQLDPKQTVRQAVEEGLGEVFEAKQ
                     KLEAIYAAYAEPDADFDKLAAEQAKYEAIVATGSGDNVEHQLEIAADSLRLPPWDAKI
                     EKLSGGEKRRVALCRLLLSKPDMLLLDEPTNHLDAESVEWLEQFLQKFPGTVVAVTHD
                     RYFLDNAAQWILELDRGRGIPWKGNYSSWLEQKEQRLEVEAKQESARIKAMQKELEWV
                     RSSPKARQAKSKARIARFEELSSHEHQKQNETQEIFIPVAERLGDSVIEFKNVSKGYG
                     DRLLIDKLSFKVPAGAIVGIIGPNGAGKSTLFRMISGKEKHDSGEVVLGSTVKLAYVD
                     QSRDALANKKTVWEAISNGQDILTVGKFEMPSRAYAGRFNFKGADQQKVVGNLSGGER
                     GRLHLATTLIAGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVLVISHDRWFLDRIA
                     SHILAFEGDSQVVFFDGNYQEYEADKVKRLGEEGAKPRRLRYKPLK"
     gene            2914..>4581
                     /locus_tag="VE030_11830"
     CDS             2914..>4581
                     /locus_tag="VE030_11830"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HZF24142.1"
                     /translation="MWFIGLFAGLLLGSMLVGGYGTLWGGLLGAAAGVLISYFRGQSS
                     QPEFEARLAALEAAVSELRKKMSQSPGTAAPSAAVRPEPVTAAPVAELTPEILQAEDI
                     SPVPTPPVAAEAAAESSTPEPAYAEPASRQLAEPSFFGRAWNWLTGGNALVRVGVVVL
                     FFGVAFLLKYAYEHTQVPIEVRLIGVALGAVIMLVIGWRLRERKPVYALAMQGGGVGL
                     LFLTVFGAFRLFSLIPGEAAFFALVAIAVLSAVLAVLQNALSLAVLGVSGGFLAPVLA
                     STGGGSHVMLFSYYAVLNAGVLGIALYRAWRPLNVLGFVFTFGIGAMWGARYYRPELF
                     ESTEPFLLLFFLQYVAIAVLFALRQAPRLRHYVDSTIVFGTPLVGFGLQSRLVHDIEY
                     GAAWGAVAVSAIYLVLASVLYRRHAETLRMLVEAFLALGVIFATLAIPLAFDGRWTSA
                     AWALEGAAAYWVGVKQGRRLPRAFGLLLQFAAGIAFVVDAGRSFSDTPILDSFYLGCV
                     FIAAAALFSNWTFGRYIEKANHLEMQVSIIVFIWGLAWWTFGGLYEIG"
BASE COUNT          850 a         1407 c         1423 g          901 t
ORIGIN      
        1 gcgaaatact ggggcgcgat cgccaatcat tccggcgacg cgtatttcga tttcgtgtac
       61 tggcacgact ggccgaacac gctcaacgag ctcgccaagc atcgcgtgcc caagcgaaag
      121 gccggcgcct acgacgcgct ccgcgaatcg tcccgcaagg gcctcgccga aggactggac
      181 gacgggcgca tcaaacgttt cctcgcgcac gtgtggaaaa aggaaaagct gaccacagca
      241 gaaagccatt gcatcatgaa cctgtgcatg gcggccacct acgatccgga cccgagggcg
      301 ccgcttggct tccgtgttcc gttcaacctg gaatcgggcg aggtcatcga aaaacgctgg
      361 agcaagtggc gcgagcacga cccgatccat ctcgtcgcga aataccgcga caacctgaag
      421 tcgctgcgcg gcatctacat cgattgcggt tggcgcgacc agtatcacat ccattacggc
      481 gcgcgcatcc tgtcgaagcg gctctcccag gccggcatcc ggcacagttg cgaggaattc
      541 gacgacgacc actccgacat cgattaccgg atggacgtga gcctgccgtt cctttacagg
      601 gcactgaaac cctgagactc tggagagaaa cttcattatt accttcggcg cacatgatcg
      661 ctccgagtcg tttccggtaa ttcggtattc gatttccttt ttcttgtttt tccttcgcgt
      721 gccttcgcgt cctttgcgtt gaaggcatct aatgccggcc tccggccggc tgattttcac
      781 cgagtcccga gtcctgcttc tccctcagca cacagcacgc agcactcaat gttcgtccca
      841 ccccatttcc cgcatataat tccccgcttt ccagtttcgc aaccgagata cggatttcat
      901 ggcccagtac gtttacacga tgcagcgggt gggcaagatc gtcccgccca aacgccagat
      961 cctcaaggat atttcgctgt ctttttttcc cggcgccaag atcggcgtgc tcgggctcaa
     1021 cggttcgggc aaatccagcc tgctcaagat catggccggc ctggaccagg aaatcgaggg
     1081 cgaagccatc ccgatgcccg acctgcgcat cggtttcctg cctcaggagc cgcagctcga
     1141 tcccaagcag acggtgcgcc aggccgtcga ggaaggtctc ggcgaagtct tcgaagcgaa
     1201 acaaaagctc gaagcgatct acgccgccta tgccgagccc gacgccgact tcgacaagct
     1261 ggccgccgag caggcgaagt acgaagccat tgtggccacc ggttccggcg acaacgtcga
     1321 gcaccagctc gagatcgccg ccgactcatt gcgcctgccg ccgtgggacg cgaagattga
     1381 aaagctctct ggcggcgaaa aacgccgcgt ggcgctgtgc aggcttttgc tgtccaagcc
     1441 cgacatgctg ctgctggacg agcctaccaa ccacctcgac gccgagagcg tggagtggct
     1501 ggagcagttc ctgcagaaat ttccgggaac cgtggtggcg gtgacccacg atcgatattt
     1561 cctcgacaac gcggcgcagt ggatcctgga actcgaccgc ggccgcggca ttccgtggaa
     1621 aggcaactac agttcctggc tggaacagaa ggaacaacgc ctggaggtcg aggccaagca
     1681 ggaatccgcc cgcatcaagg cgatgcaaaa ggaactggag tgggtgcgtt ccagtcccaa
     1741 ggcgcgccag gccaaaagca aggcacgcat tgcgcgtttc gaggagttgt cgtcccacga
     1801 gcatcagaag caaaatgaaa cccaggaaat tttcattccg gtcgcggagc ggctgggtga
     1861 cagcgtcatc gaattcaaga acgtgagcaa gggctacggc gaccgtctgc tgatagacaa
     1921 gctgagcttc aaggtcccgg ccggcgcgat cgtcggcatc atcggcccga acggcgccgg
     1981 caagtccacg ttgttccgga tgatctcggg gaaggagaaa cacgacagcg gcgaagtggt
     2041 gctcggctcc acggtcaagc tggcatacgt ggaccagagc cgcgatgcgc tcgccaacaa
     2101 gaagacggtg tgggaagcga tctccaacgg ccaggacatc ctcaccgtcg gcaaattcga
     2161 aatgccgtcg cgcgcctatg ccggccgctt caacttcaag ggcgccgacc agcagaaagt
     2221 cgtcggcaat ctctccggcg gcgagcgcgg ccggctgcat ctggccacga ccttgatagc
     2281 cggcggcaac gtgctgctgc tcgacgaacc ctccaacgac ctcgacgtgg aaaccctgcg
     2341 cgcgctggaa gatgcactgc tggaattcgc cggcagcgtg ctcgtcatat cccacgaccg
     2401 ctggttcctc gaccgcatcg cctcgcacat cctggcgttc gaaggcgact cgcaggtggt
     2461 gttcttcgac ggcaactacc aggaatacga ggccgataag gtgaagcggc tgggtgaaga
     2521 gggcgccaag cccaggcggc tgcggtataa gccgctgaag taggactcaa taagcaccaa
     2581 gcgcaaagga cgccagggac gcggaggaaa accgggggag agataaatct cgacgggaac
     2641 atgcaatggt tgccagttgg gctcacgaga tatgctttgt accccgacta gcttttgacc
     2701 ttccttggcg tccctggcgt cctttgcggt caatggttta cttcctcgag cagaatgatg
     2761 tattcccgag ccgcgccgcg caggtgccga ccggcgcgat ttttttgtgc ccgtgttgcg
     2821 gttccgcgtt atgccgatgg gctgggcgga ccgcgagatg tatgcaaaga tgccgggcca
     2881 ttggccacgc gctgttgacg ggagaatgac gccatgtggt tcatcgggct gtttgccgga
     2941 ctccttctcg gaagcatgct cgttggcggc tacggcaccc tctggggcgg tctgctcggt
     3001 gccgcggccg gggtgctgat ctcctatttc cgcggccaat ccagccaacc cgaattcgaa
     3061 gcacggctcg ctgcgctgga agctgcggtg agcgaactgc gcaagaagat gtcccaatca
     3121 cccgggacag cggcgccttc agcagcggtc cggcccgagc cggtcactgc ggctccagta
     3181 gcggagctga cgcccgaaat cctgcaagcc gaagatattt ctccggttcc cacgccaccg
     3241 gtcgccgccg aagcggcagc cgaatcctcc acacccgaac ccgcctacgc tgaacccgct
     3301 tcccggcagc tggccgagcc ttcgttcttc ggccgcgcat ggaactggct gaccggaggc
     3361 aatgcgctgg tgcgggtcgg cgtggtggtg ctcttcttcg gcgtcgcctt cctgctcaag
     3421 tacgcctacg agcacaccca agtgccaatc gaggtgaggt tgatcggcgt ggcgctgggc
     3481 gctgtaatta tgctggtgat cggctggcga ttgcgcgagc gcaagccggt ctatgcgctg
     3541 gcgatgcagg gcggcggagt gggactgctc tttctcacgg tgtttggcgc gtttcgcctg
     3601 ttcagcttga tcccgggcga ggcggcgttc ttcgcgctcg ttgccatcgc agtcctctcg
     3661 gccgtacttg cggtgctaca gaacgcgttg tcgctggcag tgttgggcgt gtcgggcggc
     3721 ttcctggccc cggtgctcgc atcgacgggg ggcggcagcc atgtgatgct gtttagctat
     3781 tacgccgtgc tcaatgccgg agttctgggt attgcgctgt accgcgcgtg gcgcccgctc
     3841 aacgtacttg gatttgtctt cactttcggc attggcgcca tgtggggggc gcgctactac
     3901 cggccggagc tgttcgagag caccgagccg tttctgctgt tgttcttcct gcagtacgtt
     3961 gcgatcgcgg tgctgttcgc gttgaggcag gcgccgcgac tcaggcatta cgtcgacagc
     4021 acgatcgtgt tcggtacccc gctggtcgga ttcggcctgc agagccggct ggtacacgac
     4081 atcgagtacg gcgccgcgtg gggtgcggtc gcggtgtccg cgatttatct ggtgctggcc
     4141 tcggtgctct accgccgcca cgccgaaacc ctgcgcatgc tggtcgaggc gttcctcgcg
     4201 ctgggcgtga ttttcgcaac gctggcgatc ccgctcgcct tcgacggccg ctggacgtcg
     4261 gcggcctggg cgctagaggg tgcggctgcg tattgggtag gtgtgaagca gggcagaagg
     4321 ctcccgcgcg ccttcggctt gctgctgcag ttcgcggctg gtatcgcctt cgtcgtcgat
     4381 gccggccgga gcttcagcga cacgccgatc ctcgacagtt tctatctcgg gtgcgtattc
     4441 atcgccgctg cggcgctgtt ttccaactgg acgttcggac gctacatcga aaaggcaaat
     4501 catctcgaaa tgcaggtatc gatcatcgta tttatctggg gcctggcctg gtggacattc
     4561 ggcggcctgt acgagatcgg c
//
LOCUS       DASQOP010000298         2836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1766099, whole genome shotgun sequence.
ACCESSION   DASQOP010000298 DASQOP010000000
VERSION     DASQOP010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1766099"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="VE030_11835"
     CDS             complement(<1..991)
                     /locus_tag="VE030_11835"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZF24143.1"
                     /translation="MLRKIRRAALLGAAAFAAAGTAYSQNEQFIPMNGYWVGPYAAGG
                     SGIFGGFIDYVNMVNARDGGVNGVKLTYEKCETEYNNARGVECYERTKKRGPTGASLV
                     HPLSTGITYSLIDKGTADHVPIVSIGYGRTDASDGRVFPWVFPLITNYWSQNTAKIKF
                     IGMKEGGMDKLKGKKIVNVYHDSAYGKETIPVLDAQAAKYGFQVTHIPVAHPGNEQQA
                     QWLQVRQIKPDWVILRGWGVMNPTALKAAAKIGFPRTKIVGVWWSGAEEDVIPAGDAA
                     KGYIAAAFNVSGGNYPVIKEIEKFVYAKGKGEMEDRSRVGSIYYNRGVVFGIIS"
     gene            complement(1261..2685)
                     /locus_tag="VE030_11840"
     CDS             complement(1261..2685)
                     /locus_tag="VE030_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24144.1"
                     /translation="MKKGFGAVLALFAAILPAGFILAQSQGLEQKIWTLLDDGKAAYV
                     SLAESDLRALGAGLDLPDGGRSITALAGKADGGAFDPRDVAKLPAQQLGHKVDWIVER
                     FRRYNFDWDITALRLTSLNPEAKNYPWFIIINGGAANFYEFYVDLKNRPGWAQYLAQK
                     LNVMIVTIPGSFKYGGWDLPVLDARRQPAYLLDRDLSMDEYEMRNAIYTNSLILQGLK
                     ALVTRHTSGDILLIGHSTSGELSMLAYEDPDLRARLKGRYLGWGSGGPARLELLRKVR
                     GKEIVSRAGASGQAGKTPLHVLERRDPPSYARGYSGFLNPLYEVGMTHLQIAERWLAA
                     EARRRPNFKQQLQNLEHGADLGEKAWVENEIESLLKRTGNPWRIAVEDVEKDLYATNF
                     TRMDGFQRMVWTTAKLDRNHWSAEEPMRSIEVFIANEYRAKNPDAQVRLINWDLPMTH
                     YGHLELPQQLAAAHYSVVRWLVRP"
BASE COUNT          507 a          881 c          865 g          583 t
ORIGIN      
        1 tggagatgat gccgaacacc acgccgcggt tgtagtagat gctgccgacg cgcgagcggt
       61 cttccatctc gcctttgccc ttggcgtaga cgaacttctc gatctccttg atcaccggat
      121 agttgccgcc ggaaacgttg aacgccgcgg cgatgtagcc cttggccgca tcgcccgccg
      181 ggatcacgtc ctcctcggcg ccgctccacc acacgcccac gatcttggtg cgcgggaagc
      241 cgattttcgc ggcggccttg agcgcggtcg gattcatcac gccccagccg cgcaggatca
      301 cccagtcggg cttgatctgg cggacctgca gccactgcgc ctgctgctcg ttgcccggat
      361 gcgcgaccgg aatatgcgtg acctggaagc cgtacttcgc agcctgtgcg tccagcacgg
      421 gaatcgtttc cttgccgtag gccgagtcgt gatagacgtt tacgatcttc ttgcccttga
      481 gcttgtccat cccgccttct ttcatgccga tgaacttgat cttggcggtg ttctggctcc
      541 agtagttggt gatgagagga aagacccacg ggaacacgcg cccgtcggag gcgtcggtgc
      601 ggccgtagcc gatcgacacg atcggcacat ggtccgccgt gcctttgtcg atgagcgaat
      661 aggtgatgcc ggtcgagagc gggtgcacga gggacgctcc ggtcggcccg cgcttctttg
      721 tgcgctcgta gcactccacg ccgcgcgcgt tgttgtattc ggtctcgcac ttctcgtagg
      781 taagcttcac gccgttcacg ccgccgtcgc gcgcgttcac catgttgacg tagtcgatga
      841 agccgccaaa gatgcccgag ccccccgcgg cgtacggtcc gacccagtaa ccgttcatgg
      901 ggatgaactg ctcgttctgc gagtaggcgg tgccggcggc cgcgaatgcc gcggcaccca
      961 agagcgcggc tcgtctgatt ttccgcaaca ttggaaccct cctttggatg gcaaatctcc
     1021 ctgtttctac aggttatcgg aatgaacagc cggcctacta tagcccaatt gccgggctgg
     1081 gaaaaccggc ccggttcagt gcgggaaagg ccagagcctc agcttttcct tggcgatttg
     1141 ccacaggcgc gccaggccca gtccggatgc gacgatccgc aagaaaatac tgatggacaa
     1201 tccggccaag ctctacgcgt ttggataaat caggagaggg aaccagatcg ccttcaccga
     1261 tcacggcctt accagccagc gaacgacgct gtaatgcgcc gcggcgagct gttgcggaag
     1321 ctccaggtgc ccgtaatgcg tcatcggcag gtcccagttg atcaaccgta cctgcgcgtc
     1381 cggatttttc gcccggtatt cgttcgcgat gaacacctca atgctcctca tcggctcctc
     1441 ggcactccag tggttgcggt cgagtttcgc cgtcgtccag accatacgct ggaaaccgtc
     1501 catgcgggtg aaattggtgg cgtacagatc cttctcgacg tcctcgacag cgatgcgcca
     1561 gggattgccg gtgcgcttga gcagggattc gatctcgttc tccacccacg ccttttctcc
     1621 gaggtcggcc ccgtgctcga ggttctgcag ctgctgcttg aagttgggac ggcggcgcgc
     1681 ctccgccgcg agccagcgtt cggcgatctg aagatgcgtc atgccgactt cgtagagcgg
     1741 gttgagaaag ccgctgtagc cgcgggcgta gctcggcggg tcgcggcgtt ccagaacgtg
     1801 gagcggcgtc tttcccgcct ggccggaagc gccggcacgg gacacgattt ccttgcccct
     1861 gaccttgcgc aacagctcaa gccgcgccgg tcctccgctg ccccagccca ggtaacgacc
     1921 cttgagccgg gcgcgcaggt ccggatcctc gtaggcgagc atcgaaagct cgccggaggt
     1981 cgaatgcccg atcagcagaa tgtcgcccga cgtgtgcctg gtcaccagcg cctttagccc
     2041 ctgcagtatc aggctgttgg tgtagatggc gttgcgcatc tcgtactcgt ccatggacag
     2101 atcgcgatcg agcaaatatg cgggctggcg cctggcgtcc agaaccggca ggtcccagcc
     2161 gccatatttg aagctaccgg gaatggtcac gatcatgacg ttgagcttct gcgccaggta
     2221 ttgcgcccag ccgggacggt tcttcaggtc gacgtagaat tcgtaaaaat tcgccgcgcc
     2281 gccgttgatg atgatgaacc acggataatt tttcgcctcc ggattgaggc tggtgaggcg
     2341 cagggccgtg atgtcccaat cgaagttgta gcggcgaaaa cgctcgacga tccagtctac
     2401 cttgtggccg agctgctgcg cgggaagctt ggcgacgtcg cgcggatcga aggcgccgcc
     2461 gtcagccttc cctgccagcg cagtgatcga tcttccgccg tccggcagat cgagacccgc
     2521 gccgagggcg cgcaaatcgg actccgccag cgaaacgtac gccgccttcc cgtcgtcgag
     2581 cagagtccag atcttctgtt cgagaccttg ggattgcgcc aggatgaatc cggccggaag
     2641 gattgccgcg aaaagagcga ggaccgcgcc gaagcctttc ttcatgttgg tgtctccccg
     2701 tgaaaaccgg tgaccacccc ggtttgaggt caccaattat ggacttaaat ttgcaaggga
     2761 ttgatataaa tgcgacgggt ctcatcatag ggacggttcg cattctagcg aaatgcgata
     2821 atcgcgcttc gcttcg
//
LOCUS       DASQOP010000299        10773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2971411, whole genome shotgun sequence.
ACCESSION   DASQOP010000299 DASQOP010000000
VERSION     DASQOP010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10773
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2971411"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(423..578)
                     /locus_tag="VE030_11845"
     CDS             complement(423..578)
                     /locus_tag="VE030_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24145.1"
                     /translation="MKDILGILGKALETVRPKGTAALAAARRELRGAAGDLRSIYGRS
                     WSDRGHL"
     gene            785..1552
                     /locus_tag="VE030_11850"
     CDS             785..1552
                     /locus_tag="VE030_11850"
                     /inference="COORDINATES: protein motif:HMM:NF019032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HZF24146.1"
                     /translation="MNAQSTSGASAKPIARWLAVIGLVLAVVCAAGAVLSGIGYRMDL
                     WHFRTGFMIIRWAFFGALAAVLLSIIGLILAGGKPRQVLVIGLLGLVVGAVTAYIPWN
                     WKQTLDAHPYIHDITTDVKNPPKFVAAIALRKEGDNTVEYDGPEVAEQQRKAYPDIMT
                     LIVPAEKDKVFAAAEAALRAMKLKVVDVNAAEGRIEATDTSMLYGFTDDVVVRVVADG
                     AGSKVDVRSKSRVGRSDFGQNAKRIRTFLRELQARQG"
     gene            complement(1604..2005)
                     /locus_tag="VE030_11855"
     CDS             complement(1604..2005)
                     /locus_tag="VE030_11855"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HZF24147.1"
                     /translation="MRSVSILLVTALCAPLGAAPADWLVIAPHHDHVTMVDRQSFRRD
                     GDRSEAWLMHNHDRPVNAPGLDRPYQSQRIHMLFDCGKRLVGTDQRVFHSGAHGRGSA
                     VANEQVKPDALRMAAPETQLETMLLEMACAA"
     gene            complement(2103..2525)
                     /locus_tag="VE030_11860"
     CDS             complement(2103..2525)
                     /locus_tag="VE030_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24148.1"
                     /translation="MADIEIPVGGGMVFAKAPDPSLVTTTHIIYGLHALSILIGLTSA
                     ITVVGAFVFGIPSIIAVIINYVNQANARGTYLESHFRWQIHTFWFALMWILIGVVLFL
                     TIIGIPLAWAVLVGAGIWVIYRIARGWLTLQDGKPMVY"
     gene            complement(2605..3879)
                     /locus_tag="VE030_11865"
     CDS             complement(2605..3879)
                     /locus_tag="VE030_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HZF24149.1"
                     /translation="MSEPLKKTDDQSFLRKLGPGLITGAADDDPSGIATYSQAGAQFG
                     LNMLWTALITFPLMVGIQTVSARIGCVTGEGLAANIRRLFPRWVLLVVVSLLVIANTI
                     NIAADIAAMGEALQLVIGGGQHGHAVVFGVLSLSLQVFIPYQRYVRLLKWLTLSLLAY
                     VVVAFSVHIDWIQAIRTSLAPELSFNPEFVAVVVAVFGTTISPYLFFWQASQEVEELR
                     GGNGSAGLNSSPEEARRHLHRIKIDTYVGMGFSNLIAFFIILSTAVTLHVAGVTQIQT
                     SAQAAEALRPLAGNFAFLLFSLGIIGTGMLAVPVLAGSAAYAMADSFGWRQGLDRKLL
                     EAKEFYGIIILATAGGVLLDFTPVDPIKALFWSAVINGVIAVPIMAVMMLMAVRPEVM
                     GPFVIRRSLKVLGWLSVLLMLVAVVAMFITSW"
     gene            complement(3994..7101)
                     /locus_tag="VE030_11870"
     CDS             complement(3994..7101)
                     /locus_tag="VE030_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZF24150.1"
                     /translation="MSIAEIFVRRPVMTGLLMIGILAFGIVSYRLLPVSDLPNIDFPT
                     IAVTSSLPGASPETMASAVATPLEKEFSTIAGLDSMDSTNAQSISRITLQFSLDRNID
                     AAAQDVQAAITRALKQLPPQMTTPPAFQKVNPADAPIFYLALTSATLPMSTVDEYAQT
                     LLAQRISTISGVAQVQVIGSQKYAVRVQLDPKALASRGIGIDEVAGAINRHNTNLPTG
                     TLYGAHRAYTVESNGQLLNAAAFRPIVIAYRNGAPVRLSEIGRVIDSVENDKLAAWLN
                     GERGIVLAIQRQPGTNTIEIVNSIKNVLPTFRAQAPAGLNMEILYDRSESVRESVNDV
                     QFSLLLALALVVLVIFLFLRNISATLIPSLALPMSIAGTFSVMYLLGYSLDNLSLMAL
                     TLCVGFVVDDAIVMLENISRHMEMGKPPMQATLEGAREVGFTIVSMTLSLAAVFIPVL
                     FMGGILGKLLHEFAVTIMAAVLVSGFVSLSLTPMLCSRWLRPVAHQRHGRIYRWSERG
                     FDAVRDVYDSTLKWSIRNRRVTMLVFFAVFGVTAWLFSAMPKGFLPSTDTGQLLIFTE
                     AAQDISFDAMAAKQRQAARIVQADPNVLAAMAFIGPSSSGSSQTLNLGRIFIRLKPRK
                     ERLPADAIIQELRPKLTSIPGMKVYLQNIPPIRIGGKVTKSEYQYTLQDADTQELYHW
                     APTLEAKLRSLPDFIDVNSDLQITNPTVKVQIDRDKASSLGVTAEQIEDALYSAFGSR
                     QVSTIYTPSNEYWVILELAPEYQSSPEALSLLYVRSGTGTLVPLSSVARVERGVSPLT
                     VTHQGQLPAVTLSFNLKPGVSLSQAISQLNKLVTELRIPPTVIGSFQGTAQAFQSSLA
                     GMGMLLIMSIFVIYLILGILYESYIHPVTILSGLPTAGFGALLTLMLFKLDLNMYGFV
                     GLIMLIGIVKKNAIMMIDFAIEAQRKDGKPPEQAIYEACLIRFRPIMMTTAAALMGTL
                     PIAMGLGAGSEARKPLGLAVVGGLLVSQLLTLYITPVIYLYLERARTWNERRRGRSAA
                     VTAGA"
     gene            complement(7098..8255)
                     /locus_tag="VE030_11875"
     CDS             complement(7098..8255)
                     /locus_tag="VE030_11875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZF24151.1"
                     /translation="MTIRPIWRSAANATRILAVAVPALLAACLLDACSDKSGGAQAEA
                     KKSVPTVPVLIGQAVEKPMPLRLHAIGNVETVASVAVKSRVDGQIIATHVRDGQDLVK
                     GDPLFQIDQKPYQSQLEQAQANLAHDQAQLEYLRGQEQRYQDLLKQNFVSKEGYAQVA
                     ANFRGAEASVSADEAAVQHARINLGYTTIRSPIGGRAGKVMLSEGNMVKANDTVAMVV
                     INQLAPIYVSVAVPEQYLNEIRARHKNAPLRVEVELEKGKTISGKLAFVDNSVDVTTG
                     TIKLRGIFSNDDKQLWPGQFVESWVTLRDEEKALVVPTQALQTGPNGQYVYVVKEDFS
                     VELRPVQVARTEGAETVIAGGLAAGETVVVDGASRILPGSKVSAKTPEKPS"
     gene            complement(8381..9853)
                     /locus_tag="VE030_11880"
     CDS             complement(8381..9853)
                     /locus_tag="VE030_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007740792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZF24152.1"
                     /translation="MLFALVPAFALLASCTVGPNYVRPTADTPATFKEMEGWKTAQPR
                     DQELRGKWWEAFNDPLLNDLQEQVSLSNQNLAQAAAQFRQARALVQSARAGYWPGVTA
                     GASATRSQSPSGFVSQNQNLNVRGPSTSYSLSLDAVWEADLWGRVRRTVESNEAGAQA
                     SAADLEALRLSIQAELAQNYFQLRALDAQKQLFDATIAAYQRSLALTQNQYAAGVVAK
                     VDVIQAQTQLRTTQAQALDIGVQRAQLEHAIALLIGKPASSYSLAAAALAATPPALPV
                     GMPASLLERRPDIAAAERRMAAANAQIGVAKAAYYPSLTLSASGGFQSSSFSNWLTAP
                     SRFWSLGPALAQTLFDGGLRRAQTEQAIAAYDANVAGYRQTVLTGFKEVEDNLAALRI
                     LEEEAAIQEDAVQNARQSVALTTNQYKAGIVSYLNVVTVQATALANERAAVDILNRRL
                     AASVLLIKALGGGWDASALPLPDELARSDATPGKGAAQTR"
     gene            complement(9964..>10773)
                     /locus_tag="VE030_11885"
     CDS             complement(9964..>10773)
                     /locus_tag="VE030_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZF24153.1"
                     /translation="DVFVSNANGKQVPLSAFSSYGPTSTPLAVNHQGQFVASTISFNL
                     PVGVALSDATRAIADTLNRIGVPTSIQGSFQGTARAFQASLNSQPWLILTALLTVYIV
                     LGMLYESFVHPITILSTLPSAGVGALLALLVCGTEFSIIALIGVILLIGIVKKNAIMM
                     IDFALDAERRLGMTPEQAIYQACLLRFRPIMMTTMAALLGAVPLAIGFGDGAELRRPL
                     GISVVGGLIVSQMLTLYTTPVVYLYLDRFRLYCKRVFARRMRPVEMAGDTA"
BASE COUNT         2026 a         3280 c         3490 g         1977 t
ORIGIN      
        1 gtggcaggtt atcccagagg cgggacaaaa aacagggttg tccggacatt cgtgtgggtg
       61 atcggcgtcc gggtgttgaa gaattgatcg ctttgcggaa cctttcgtcg ccaaaccttt
      121 aaggccaagc gttaaccgcc agggacgcca tggaaagaca atagcgtgaa gtcgaggcgg
      181 agatagggta acgggccggt cgcaacgcag atacttgcac agggtttgca cactcacttg
      241 attcttgcat tttgactttc cctggcgtcc ttggcgtcct tggcgtccct ggcggtgaat
      301 gcgttattgg tgagtcgttc gcgactcaca gagaagtctg aagaaaaaaa aagagggccg
      361 cgaaggccct cgaactacct gcaaggaaag acagcgggga ttgaagagtg gacaggcggc
      421 gactacaggt gaccccggtc gctccagctg cgcccgtaaa tgctgcgaag atcgccggcc
      481 gcgccgcgca attcgcgccg cgctgcggcc agtgcagcgg tgcctttcgg gcgaaccgtt
      541 tcgagtgcct tgccaagaat gccgagtatg tctttcatga tcgacctcca gatccgttga
      601 ttccggcgca catccgccgg atgcatacac aatgtagtgt tggagccgcc gccggggaag
      661 gccgcgcgcg cgcatagcat ctatgccctg cgcgcatcgt tggccgcggg gttagaatgc
      721 tctccgaatg cgaggcccgg cggccgcagt tgccggctgt tttccgtcag aacaaggagg
      781 ggatatgaac gcacaatcga cctcaggcgc ttccgccaaa ccgatagcca ggtggctggc
      841 ggtcatcgga ctggttctcg cagtggtatg cgcggcgggg gccgtgctgt ccggaatcgg
      901 ctaccggatg gatctgtggc atttccgcac cggcttcatg atcatccgct gggcgttctt
      961 cggcgcactc gccgcggtgt tgctgtcgat catcggcctg atcctcgccg ggggcaaacc
     1021 gcggcaagtc ctggtgatcg gcctgctggg actcgtggtc ggtgcagtga cggcgtatat
     1081 cccgtggaac tggaagcaga cgctggacgc gcatccctac atccacgata tcaccaccga
     1141 cgtcaagaat ccgccgaaat tcgtcgcggc gatcgcgctg cgcaaggaag gcgataacac
     1201 ggtggaatac gacggaccgg aagtggccga gcagcagcgc aaggcctatc ccgatatcat
     1261 gacgctgatc gttcccgcgg aaaaagacaa ggtgttcgcg gccgccgagg cggcgctgcg
     1321 cgccatgaag ctgaaggtgg tcgacgtgaa cgccgcagaa gggcggatcg aggccaccga
     1381 caccagcatg ctctacggct tcactgacga tgtcgtggtg cgcgtggttg cagacggcgc
     1441 aggcagcaag gtggacgtgc gctccaagtc gcgggtcggg cgcagcgatt ttggccagaa
     1501 cgccaagcgc atccgcacgt tcctgcggga actgcaggcc agacagggat gaaatgaatt
     1561 gcgctccccg gggagccctt tatcaaatct tcatcattgc gattcaggcc gcgcaagcca
     1621 tctccagcag catcgtttcc aactgagtct ccggcgcggc catgcgcaac gcgtcgggct
     1681 tgacctgctc gttcgccacc gcgcttccgc ggccatgcgc gccgctgtgg aatacgcgct
     1741 gatccgtgcc gaccaggcgc ttgccgcagt cgaacaacat gtgaatgcgc tgcgattggt
     1801 aaggacgatc gagccccggc gcgttgacgg gccggtcatg gttgtgcatc aaccacgcct
     1861 ccgatctgtc gccgtcgcgc ctgaaactct gccggtccac catcgtcacg tggtcatggt
     1921 ggggcgcgat gacgagccag tccgctggcg cggcaccaag tggcgcgcat agtgccgtta
     1981 caaggaggat cgagaccgat cgcatggttt gttgttgtgg gattccggca agacgcccgc
     2041 aagtcctcgc aggtgcgtta cgggaaggat ccggaccggc ccgacggcgg gcgggcgcgg
     2101 gttcagtaca ccatcggctt gccgtcctgc agcgtcagcc agccacgcgc gatgcggtag
     2161 atcacccaga ttcccgcgcc gaccaggacc gcccacgcga gagggatgcc gatgatcgtg
     2221 aggaaaagca cgaccccgat cagtatccac atcagcgcga accagaaagt gtggatctgc
     2281 cagcggaagt gggattcgag atacgtgccg cgggcattcg cctggttcac gtagttgatg
     2341 atgacggcga tgatcgaggg gatgccgaaa acgaatgcgc cgacgacggt tatcgcgctg
     2401 gtaaggccga tgaggatgct cagcgcgtgc aagccgtaga tgatgtgggt ggtggtaacg
     2461 agcgacgggt ccggggcctt tgcgaatacc atgcctccgc cgacaggaat ttcaatgtcc
     2521 gccattacgc gattctcctc ctcatgcccg ggcaggccgt gaccggatta tggcatggcg
     2581 accgcagagg cgcgcagccg atgatcacca ggacgtaatg aacatcgcta caacggcgac
     2641 cagcattagc agtaccgaca gccagccgag gaccttgagg ctgcgtctga tcacgaaagg
     2701 acccatcacc tccgggcgca ccgccatcag catcatcacc gccatgatcg gcactgcgat
     2761 gacgccgttg atgaccgcac tccagaacaa ggccttgatc ggatcgaccg gcgtgaagtc
     2821 aagcagcacc ccgccggctg tggccagaat gatgatcccg tagaactcct tggcctcgag
     2881 caacttgcgg tccagtccct gtctccagcc gaagctgtcg gccattgcgt aagcggccga
     2941 gccggccagc accggcaccg ccagcatccc ggtgccgatg atccctaggc tgaacaacag
     3001 gaatgcgaaa ttgcccgcga gcgggcgcag cgcctccgca gcctgtgccg aggtctggat
     3061 ctgggtgacc ccggctacgt gcagcgtcac cgccgtgctg aggatgatga aaaaagcgat
     3121 caggttggaa aagcccatcc cgacgtaagt gtcgatcttg atccggtgca ggtgccggcg
     3181 cgcctcttcc gggctgctgt tgagcccggc cgaaccgttg cccccgcgca gttcctccac
     3241 ttcctgggaa gcctgccaga agaacaggta gggactgatg gtggtgccga acaccgcgac
     3301 cacgacagcg acgaactccg gattgaacga gagttccggc gccagcgacg tccggatcgc
     3361 ctggatccag tctatgtgca ccgagaacgc gaccaccaca tacgccagca gcgacagcgt
     3421 cagccatttc aacaggcgca cgtagcgttg ataggggatg aagacctgaa gcgacaggct
     3481 cagcacgccg aagacgacgg catggccgtg ttggccgccg ccgataacca gttgcagggc
     3541 ttcgcccatc gcggcgatgt ccgccgcgat gtttatggta ttggctatga cgagcagcga
     3601 aacgacgacc agcaagaccc agcgcggaaa caggcggcgg atgttggcgg cgaggccttc
     3661 tcccgtgacg cagccgatgc gcgcgctgac ggtctggatg ccgaccatca acgggaaggt
     3721 gatcagcgcc gtccagagca tgttcaatcc gaactgcgcg ccggcctgag aataggtggc
     3781 gatgccgctc ggatcgtcgt ccgcggcgcc ggtgatcagt ccgggaccga gcttgcgcag
     3841 aaacgattgg tcgtccgtct tcttcagcgg ttcgctcatg tgttctccgg gaacgagcac
     3901 agggcgaacg aagcgggtcg cgtggtgccg cggctctaac gccagccgga gaatatcgtt
     3961 gacgtcacct acggcgggcc aacgaacacg ccgtcaggcg cctgcggtca cggccgcgct
     4021 gcggccgcga cgacgctcat tccaggttct ggctctctcg aggtaaaggt agatcaccgg
     4081 cgtgatgtac agggtcagca gctgcgacac cagcaatccg ccgacgaccg cgaggcccag
     4141 cggcttgcgc gcttccgatc cggcgccgag acccattgcg atcggcagag tgcccatcag
     4201 tgctgcggcc gttgtcatca tgatcggccg gaaccggatc aggcaggcct cgtaaatcgc
     4261 ctgttcgggc ggcttgccgt ccttgcgctg cgcttcgatg gcgaagtcga tcatcatgat
     4321 ggcgttcttc ttcacgatgc cgatcagcat gatgagcccg acgaagccgt acatgttgag
     4381 gtcgagcttg aacagcatca gcgtcagcag cgcaccgaag ccggccgtcg gcagtccgga
     4441 cagaatggtg accgggtgga tgtaactctc gtagagaatg cccaggatca ggtagatcac
     4501 gaagatcgac atgatcagta gcatccccat gccggccagc gacgactgga atgcctgcgc
     4561 cgtgccctgg aaactgccga ttaccgtggg cggaatgcgc agctcggtga cgagcttgtt
     4621 gagctggctg atcgcctggc tcagcgacac acccggtttc aggttgaacg acaacgtcac
     4681 cgccggcaac tgtccctggt gggtgaccgt caacgggctg acgccgcgtt cgacacgcgc
     4741 caccgaagac agcggcacca gcgtgcccgt gcccgatcgc acgtagagca aggacaaggc
     4801 ttcggggctg gactggtact cgggcgccag ttccaggatg acccagtact cgttgctcgg
     4861 cgtgtagatg gtcgacacct ggcgcgagcc aaatgcactg tagagggcgt cctctatttg
     4921 ttctgcggtc acgcccaggg aggaggcctt gtcgcggtcg atctgcacct tcaccgtcgg
     4981 attggtgatc tgcaggtcgc tgttcacgtc gatgaagtca ggcaggctgc gcaactttgc
     5041 ttccagggtc ggcgcccagt gatacaactc ctgggtatcg gcatcctgca gcgtgtactg
     5101 gtattcgctc ttggtcacct tgccgccgat gcgtatcggc gggatgttct gcaggtacac
     5161 cttcatgccg ggtatggacg tgagctttgg cctcagctcc tggatgatgg cgtccgcggg
     5221 cagtctttcc ttgcggggct tcaggcgtat gaaaatacgg cccaggttca gcgtctggct
     5281 cgatcccgac gaggaaggac cgatgaatgc catggcggcc aggacgttcg ggtcggcttg
     5341 cacgatccgc gccgcctgcc gttgtttcgc cgccatggca tcgaaggaaa tatcctgcgc
     5401 ggcctcggtg aagatcagca actgcccggt gtcggtactg ggcaggaagc ctttgggcat
     5461 ggcggagaac agccatgcgg tcacgccgaa taccgcgaag aacaccagca tcgtgacccg
     5521 tctgtttctg atcgaccatt tcagcgtcga gtcgtaaacg tcgcgcaccg catcgaagcc
     5581 gcgctcgctc cagcggtaga tcctgccgtg gcgttggtgc gcgaccgggc gcagccagcg
     5641 gctgcacagc atcggcgtca gcgaaagcga cacgaaaccc gacaccagca cggcggccat
     5701 gatggtgacc gcgaactcgt gcagcagctt gccgagaatg ccgcccatga acagaaccgg
     5761 aatgaacacc gcggcgagcg acaacgtcat cgacacgatg gtgaagccga cttcgcgcgc
     5821 gccttccagc gtcgcctgca tgggaggctt gcccatttcc atgtggcggc tgatgttctc
     5881 cagcatgacg atggcgtcgt cgacgacgaa gccgacgcaa agcgtgagcg ccatcagcga
     5941 aagattgtcc aggctgtagc ccagcagata cattaccgag aaagtgccgg ctatcgacat
     6001 cggcagcgcc aggctgggaa tgagcgtggc ggagatattg cgcaggaaca ggaaaatcac
     6061 cagcaccacc agcgccagcg caagcaatag cgagaattgc acgtcattca cggattcgcg
     6121 caccgattcc gaccggtcat agaggatctc catgttcagg ccggccgggg cctgcgcgcg
     6181 gaaagtcggc agcacatttt tgatcgaatt gacgatctcg atggtgttgg tgcccggctg
     6241 gcgctggatt gcgagcacga tgccgcgctc gccgttgagc caggcagcga gcttgtcgtt
     6301 ctccacgctg tcgattaccc ggccgatttc gctcagccgc accggcgccc cgttgcgata
     6361 cgcgatcacg atcggacgga atgccgccgc attcagcagc tggccgttcg attccaccgt
     6421 ataagcccga tgcgcgccgt aaagcgtgcc ggtgggcagg ttggtgttgt gccggttgat
     6481 cgcgccggca acctcgtcga tgccgatgcc gcgcgaagcg agtgcctttg gatcgagttg
     6541 cacgcgcacc gcatatttct gcgaaccgat cacctggact tgggcgacgc cgctgatcgt
     6601 ggagatgcgc tgcgcgagca gggtctgcgc gtactcgtcc acggtcgaca tgggcagcgt
     6661 cgccgaagta agggcaaggt agaagatcgg tgcgtccgcg gggttgacct tctggaaggc
     6721 gggcggtgta gtcatctgcg gcggcaactg tttcagcgcg cgcgtgatcg cggcctgcac
     6781 gtcctgtgcg gcggcgtcga tgtttcggtc gagcgagaac tgcagggtga tgcggctgat
     6841 gctctgcgcg ttggtggagt ccatcgaatc gagaccggca atcgtggaga attccttctc
     6901 cagcggagtc gccacggcgg aagccatggt ttccgggctt gcgccgggca gcgacgaggt
     6961 caccgcgatg gtggggaagt cgatgttggg aagatccgat accggcagca gccggtagct
     7021 gactatgccg aaggcgagga tgccgatcat gagtagcccg gtcatgaccg gccggcggac
     7081 gaagatctcg gctatgctca agacggcttc tccggcgttt tggcgctgac cttggagccc
     7141 ggcaggatgc gcgatgcgcc gtccaccacg acggtttcac cggccgcgag gccgccggcg
     7201 atcacggtct ccgcgccttc ggtgcgcgcg acctgcacgg ggcgcagctc gacgctgaaa
     7261 tcctccttga cgacatagac atactggcca ttcgggccgg tctgcaacgc ctgcgtcggc
     7321 acgaccagtg ccttctcttc gtcccgcagc gtgacccagg attcgacgaa ttggcccggc
     7381 cagagttgtt tgtcgtcgtt ggagaaaatg ccgcgcagct tgatggtgcc ggtggtcacg
     7441 tcgaccgaat tgtcgacgaa ggcgagcttg ccgctgatcg tcttgccttt ctcgagttcg
     7501 acctccacgc gcaacggcgc attcttgtga cgcgcgcgga tctcgttcag gtattgctcg
     7561 ggcaccgcga cgctgacgta aattggcgcg agctggttga tcaccaccat cgccaccgtg
     7621 tcgttggcct tgaccatgtt gccttccgaa agcatcacct tgccggcacg gccgccgatc
     7681 ggcgagcgga tcgtcgtata gcccaggttg atgcgcgcat gctggaccgc ggcttcgtcg
     7741 gcgctgacgc tcgcttccgc tccccggaaa ttggcggcga cctgcgcgta accctccttg
     7801 gagacgaagt tctgcttcaa caaatcctgg tagcgctgtt cctgcccgcg caggtactcg
     7861 agttgcgcct gatcatgcgc gagattggcc tgcgcctgtt cgagctggga ctgataaggt
     7921 ttctgatcga tctggaacag cggatcgccc ttgaccagat cctggccatc ccgcacatgg
     7981 gtagcgatga tctggccatc gacgcgcgat ttcacggcca cggaggcgac cgtctctaca
     8041 ttgccgattg catgcaggcg cagcggcatc ggcttttcga ccgcttgtcc tatcagtacg
     8101 gggacggtag gtacggattt cttggcttcg gcctgggcac cgccgctctt gtcggaacag
     8161 gcgtccagta aacaggccgc aagcagcgcg ggaaccgcga ccgccagaat cctggtcgcg
     8221 ttcgcggctg atcgccaaat ggggcggatg gtcacgttgc aattcctcca cacagcttca
     8281 gcggcgccgg tcctgtccgg cactcgtctt gtcgcaaatc agttgctaat cttgcgctca
     8341 tttgtttgca ctgtgtatgg gtcgcgcgcc tgccgcaacc tcatcgcgtc tgcgcggcac
     8401 ctttgcctgg agtcgcgtcg ctgcgcgcca gttcgtccgg caggggcagg gccgatgcgt
     8461 cccaaccccc gcccaacgcc ttgatcaaca gcacgctggc cgccagccgc cggttgagga
     8521 tgtccactgc ggcacgctcg ttggccaacg cggtcgcctg taccgtcaca acattcagat
     8581 agctgacgat acccgccttg tactggttgg tggtcagggc caccgactgg cgggcgttct
     8641 gcaccgcgtc ttcctgaatc gccgcttcct cttccaggat gcgcagcgcc gcgagattat
     8701 cctcgacttc cttgaagccg gtgagcacgg tctgccggta acccgcgacg ttcgcgtcgt
     8761 aggcggcgat tgcctgttcg gtctgtgcac gccgcaggcc gccgtcgaac agcgtctgcg
     8821 ccagcgccgg tccaagagac cagaaacggc tgggcgcggt caaccagttc gagaaactcg
     8881 agctctggaa gccgcccgat gcggacaatg tgagggatgg atagtaggct gctttggcga
     8941 caccgatctg cgcgttcgcc gccgccatgc gccgttcggc ggcggcgatg tcgggccggc
     9001 gttcgagcag actggcgggc atgcccaccg gtagtgccgg aggcgtggcc gcgagcgcgg
     9061 ccgcggccag tgaatagctg gacgcgggtt tgccgatcag cagcgcgatg gcgtgttcga
     9121 gttgcgcgcg ctgcacgccg atgtcgagcg cctgtgcctg cgtcgtcctg agctgggtct
     9181 gcgcctggat cacatcgacc ttggcgacga cgcccgccgc gtactgattc tgcgtcaggg
     9241 cgagtgagcg ctgatacgcg gcgatagtgg cgtcgaaaag ctgcttctgc gcatccagtg
     9301 cgcgcaactg gaaatagttc tgcgccagtt ccgcctggat cgaaagccgc aatgcttcga
     9361 ggtcggcggc gctcgcctgc gcgccggcct cgttcgactc gacggtgcgg cgtacgcggc
     9421 cccacaggtc ggcttcccag accgcatcga gcgacagcga atagctggtg ctcggtccac
     9481 gtacattcaa attctggttc tgactgacga agccggaggg agattgcgaa cgcgtcgccg
     9541 aagcgccggc cgttacgccc ggccaataac cggcgcgcgc cgattgcacc agcgcgcgcg
     9601 cctggcggaa ttgcgcggcg gcttgcgcca ggttctggtt ggaaaggctg acctgctcct
     9661 ggagatcgtt gagcagcgga tcgttgaatg cttcccacca cttgccgcgc agctcctggt
     9721 cgcgcggctg tgccgtcttc cagccttcca tttccttgaa cgtggccggc gtgtccgccg
     9781 tcggccgcac gtagttcggc ccgacggtgc aggaggcgag cagcgcgaac gccggaacga
     9841 gggcgaaaag caccaatcgc aaatcgttca ccacggcgga cacggaggcg gaaccaaacc
     9901 acggatctgg aacctgggta ctcaaaaaag ccttcctccg tgtcctccgt ggttcagtgt
     9961 ttttcatgca gtgtccccag ccatttcgac cggccgcatg cgtcgggcga aaacgcgctt
    10021 gcaataaagg cggaagcggt cgagatagag ataaaccacc ggcgtcgtat agagcgtgag
    10081 catctggctg acgatcaagc cgccgacgac cgagatgccg agcggacggc gcagttcggc
    10141 gccgtcgccg aatccgatgg cgagcggtac tgccccgagc agcgccgcca tggtcgtcat
    10201 catgatcggg cggaagcgca gcaggcaggc ctggtagatc gcctgctcgg gcgtcatgcc
    10261 cagcctgcgt tcggcatcga gtgcgaaatc gatcatcatg atcgcgttct tcttgacgat
    10321 gccgatcagc agaatcacgc cgatgagcgc aatgatgctg aattcggttc cgcataccag
    10381 cagtgcgagc agggcgccga cgccggccga gggcagggtc gacaggatgg tgatcggatg
    10441 aacaaaactt tcatagagca tgccgagcac gatgtacacc gtgagcaatg cggtgagaat
    10501 cagccagggc tggctgttca gcgacgcctg gaaggcgcgc gcggtgccct ggaagctgcc
    10561 ctgaatcgaa gtcggtacgc cgatgcggtt gagcgtgtcg gcaatcgcgc gggtggcgtc
    10621 cgacagtgca acgccgaccg gaagattgaa cgaaatcgtc gaggccacga actgtccctg
    10681 gtggttcact gcaagcggcg tgctggtcgg gccgtagctc gagaaggccg acagcggcac
    10741 ctgcttgccg ttggcattgc tcacgaagac gtc
//
LOCUS       DASQOP010000300         6796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2170748, whole genome shotgun sequence.
ACCESSION   DASQOP010000300 DASQOP010000000
VERSION     DASQOP010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6796
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2170748"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VE030_11890"
     CDS             complement(<1..393)
                     /locus_tag="VE030_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24154.1"
                     /translation="MEAAKTIEILKALAEGIDPGTGEQFPAGSTYQHPDTVRALFAAI
                     RGLENPTPRPAKTDAPQKPAPENAGRPWSQEEDSRLGQAYDSGKSVEELAQMHKRSKW
                     AIESRLARMGKIPAPPPFVPRSSPGNAVT"
     gene            complement(504..1106)
                     /gene="lexA"
                     /locus_tag="VE030_11895"
     CDS             complement(504..1106)
                     /gene="lexA"
                     /locus_tag="VE030_11895"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HZF24155.1"
                     /translation="MDDLTPRQAEILQLIADFVQSTGFPPTRAEIARSLGFRSANAAE
                     DHLKALEKKGFIELFAGASRGIRLKEGLGLPVIGRVAAGSPILAEAHVQGRYQVDPLL
                     FNPRADYLLKVRGMSMREAGILDGDLLAVHRTQEARSGQIVVARLHDEVTVKRFKRQG
                     NQVQLLPENPDFKPIYVDLKRDPLAIEGLAVGLIRNGRIS"
     gene            1516..2757
                     /gene="urtA"
                     /locus_tag="VE030_11900"
     CDS             1516..2757
                     /gene="urtA"
                     /locus_tag="VE030_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008120030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HZF24156.1"
                     /translation="MNRRQLLKSLIGIAFSAMAATATAADTIKVGILHSLSGTMAISE
                     TALKNTALMAIEEINAKGGLLGKKLEPVVVDPASNWPLFAEKARQLITQDKVAVVFGC
                     WTSVSRKSVLPVFEELNSLLFYPVQYEGEELSQNVFYTGAAPNQQAIPAVEYLMSEEG
                     GAAKRWFLLGTDYVYPRTTNKILRAFLKAKGVAETDIAEVYTPFGHSDYQTIVADVKK
                     FSAGGKTAVISTINGDSNVPFYKELGNAGLKATDVPVVAFSVGEEELRGIDTKPLLGH
                     LAAWNYFMSVKNPQNTAFIKMYKDWAKKNKLPNLDTVVTNDPMEATYVGIHMWAQAVK
                     KAGSTDVDKVRVAMAGQTFKAPSGFTLTMDKTNHHLHKPVMIGEVTGDGQFNVVWKTK
                     GPIRAQPWSPFIAGNEGKQGL"
     gene            2908..4512
                     /gene="urtB"
                     /locus_tag="VE030_11905"
     CDS             2908..4512
                     /gene="urtB"
                     /locus_tag="VE030_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HZF24157.1"
                     /translation="MKPALLVSAFVAALACAATARALDEEIVARLGAEEVGTRLEAIN
                     ALVASGDEAALALLQAMQDGQVNLTPSGRVVVVRGDKAFDAATMAPIEPEPEGLESIV
                     VNNRIRGALDTAIGALKLLSPDRAVRLASAKAIETTSQVALLPLIEKAISRESDPGIR
                     KSLQQTKAALDLKSSDPGTRLAAVSTLAESNQAQVKTRLLALLEKDASGSFVEPDAQV
                     RAAAERSIDSIQRRLLISDYLARLFTGLSLGSILLLVALGLAITYGLLGVINMAHGEM
                     LMIGAYATFVTQQLFKSYAPGLFDWYLVAAIPVAFAASALVGMALERSVIRWLYGRPL
                     ETLLATWGISLFLIQLVRQIFGAQNVEVANPSWMSGGVELTNMVLPYNRIAIIAFSMF
                     VLLMMWLMLSRTRLGLFVRGVTQNRIMASCVGVPTERVDLLAFGLGSGVAGLAGCALS
                     QIGNVGPDLGQSYIIDSFMVVVLGGVGQLAGTVYAALGLGVLSKFLEPHIGAVLTKIL
                     VLVLIIAFIQKRPQGLFALKGRAVEA"
     gene            4571..5620
                     /gene="urtC"
                     /locus_tag="VE030_11910"
     CDS             4571..5620
                     /gene="urtC"
                     /locus_tag="VE030_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HZF24158.1"
                     /translation="MALGAFALVTLVVLPLLYLLPAADSALHVSSYTITLAGKIMCYC
                     IVAMAMNLIWGYTGILSLGHGVFFALGGYAFGMYLMRQIGREGQYQSDLPDFMVFLDW
                     KAFPWFWTGSEHFLWSATLVILAPGLLALAFGYFAFRSRIKGVYFSIITQAMTFAFML
                     LFFRNDTGFGGNNGFTDFKRVLGFPITAPSTRGAIFAMTALALIGVVFLTRYLIRSKV
                     GRVLTAIRDSESRLMFSGYNPLGYKLFVWTLSAVLCGIAGALYVPQVGIINPSEMSPA
                     NSIEIAVWVAVGGRGSLIGPLLGAAVVNGAKSFFTQTFPEYWLFFLGLLFILVTLFLP
                     RGVIGLLSPARWRRT"
     gene            5617..6438
                     /gene="urtD"
                     /locus_tag="VE030_11915"
     CDS             5617..6438
                     /gene="urtD"
                     /locus_tag="VE030_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HZF24159.1"
                     /translation="MNTISEEISVSGNTSGLSHVLQGGVDITHGPILYLDDITVSFDG
                     FKALNKLTLTVEAGELRCVIGPNGAGKTTMMDVITGKTRPDEGSAYFGQTIDLARLTE
                     PEIAHAGIGRKFQKPTVFENHTVFENLELAMKTDKRVWASLRARLSAEQLDRICKTLE
                     LIKLQGEAGRLAGLLSHGQKQWLEIGMLLMQEPRLLLLDEPVAGMTDEETERTAELFK
                     SLAGERSVVVVEHDMEFIGSIASVVTVLHEGAVLAEGTMDAVKNDERVIEVYLGR"
BASE COUNT         1250 a         2036 c         2156 g         1354 t
ORIGIN      
        1 cgtcacggca tttcccggcg aagaacgcgg cacgaagggc ggcggagcgg ggatcttgcc
       61 catgcgcgcc agcctggatt cgatcgccca tttgcttctc ttgtgcattt gggccagttc
      121 ttcgacggat ttgcccgagt cgtaggcctg gccgaggcgc gagtcttctt cctgggacca
      181 ggggcgtccg gcgttttcgg gggcgggttt ctgcggcgcg tccgtttttg ccgggcgcgg
      241 cgtgggattc tccagcccgc gaattgcggc gaagagcgcg cgcacggtgt ccggatgctg
      301 ataggtgctg ccggcgggaa attgttcgcc ggtgccggga tcgatgccct cggccagggc
      361 cttgagaatc tcgatggtct ttgctgcttc catgattgcc tcctgattga tgcggttgag
      421 agatgtcatc acctgcacct gtgcgaactc accgcagaga gggtgtttgt ttttttaccc
      481 ctcactcatt cacccttcac gccttacgaa atcctgccat tccggatcag cccgaccgcc
      541 agtccttcga tcgccagcgg atcgcgcttc aggtctacat atatgggttt gaagtccggg
      601 ttctcgggca gcagttgcac ctggttgccc tggcgcttga agcgcttcac cgtgacttcg
      661 tcgtgcaggc gcgccaccac gatctggccg gaccgcgcct cctgggtgcg gtgcaccgcc
      721 agcaggtcgc cgtcgagaat gccggcctcg cgcatgctca ttccccgcac cttcaacagg
      781 taatccgcac gcgggttgaa cagcagcggg tcgacctgat agcgcccctg gacatgggct
      841 tcggcgagga tcgggctgcc tgccgcgacc cggccgatga ccggcaggcc gagtccctcc
      901 ttgagccgga tgccgcgcga ggcgcccgca aacaattcga taaagccttt tttttccaag
      961 gccttgagat gatcctcggc agcgttcgcg gaacggaaac cgaggctcct ggcaatttcg
     1021 gcgcgggtgg gaggaaagcc ggtgctctgc acgaaatcgg caatcagttg cagtatctcg
     1081 gcctggcggg gggtcagatc gtccatggga aacctcctgt actgtaatta tatacagtaa
     1141 caggggcggg agtgcaagcg gcgcgaaaac ggcggaaatt cccggtcgtt caggtcccgg
     1201 agtgggtccg cgaccttgtt gtgcatcgcc ggcccccgcc gcacaaagcc ggtgcgtgcg
     1261 tggtgtcgcc tgcgtgcctg cggcgttcca aaaccatctc ttcgggcgct ttgcggcgcg
     1321 gcaagcacat gaaatgcaaa agcattcgtt gcggccgcga agcatcggca tggttgttgc
     1381 acgacactct cgtgcaatct caaaacctgc tcgtcgcaag acgattcgaa ttgccgcagc
     1441 cagaggcgca ctgcccggca gtccgggatc cgcgagcgcc ggcggtcatt gttcaacatc
     1501 tttcagggga gttccatgaa tcgcagacaa ttattgaagt cgttgattgg aatcgcgttc
     1561 tctgcgatgg cggcaacggc gacggctgcc gacaccatta aggtcggcat tctgcactcg
     1621 ctgtcgggga ccatggcgat ttcggaaacg gcgctgaaga acactgcgct gatggcgatc
     1681 gaagagatca atgccaaagg cggcctgctc ggcaagaaac tcgaaccggt ggtggtcgac
     1741 ccggcttcga actggccgct gttcgccgag aaggcgcgcc agttgatcac ccaggacaag
     1801 gtggccgtgg tgttcggctg ctggacctcg gtgtcgcgca aatcggtcct gccggtgttc
     1861 gaagaactga acagcctgtt gttctacccg gtgcaatacg aaggtgaaga gctttcccag
     1921 aacgtgttct acaccggcgc ggcacccaac cagcaggcca ttcccgcggt cgagtacctg
     1981 atgagcgaag aaggcggcgc cgccaaacgc tggttcctgc tcggcaccga ctacgtttat
     2041 ccgcgcacca ccaacaagat cctgcgagcg ttcctcaagg ccaagggtgt ggcggagacg
     2101 gacatcgcgg aggtctacac gccgttcggc cacagcgatt accagacgat cgtggccgac
     2161 gtcaagaaat tttccgcggg cggcaagact gcggtgatct ccaccatcaa cggcgactcc
     2221 aatgtgccgt tctacaagga gttgggcaac gccgggctga aagccaccga cgtgccggtg
     2281 gtggcgtttt cggtcgggga agaggaactg cgcggcatcg acaccaagcc gctgcttggc
     2341 cacctggccg cgtggaacta cttcatgtcg gtgaagaacc cgcaaaacac cgcgttcatc
     2401 aagatgtaca aggattgggc caagaagaac aagcttccga atctcgacac cgtggtgacc
     2461 aacgatccga tggaagccac ttatgtcggc atccacatgt gggcgcaggc ggtgaagaaa
     2521 gctggctcca ccgacgtcga caaggttcgc gtggcaatgg ccggccagac cttcaaggcg
     2581 ccgtccggct tcacgctgac gatggacaag accaaccatc acctgcacaa gccggtcatg
     2641 atcggcgaag tcacgggcga cggtcagttc aacgtggtgt ggaagaccaa gggtccgatc
     2701 cgtgcccagc cgtggagccc gttcatcgcg ggtaacgaag gcaagcaagg cctgtagtcg
     2761 catcaaccgg ccgcgggcag cgcgttctcc ttgcgcgcat cccgccgtcg acaacagcat
     2821 cagggcaggg cctgcggtaa ggcagtgacg agccgcgggc cctttttttg atttttgttc
     2881 gcgcgatacg ccttaaggga aacgcgcttg aaaccagcat tgctcgtttc tgcattcgtc
     2941 gcagcactcg cctgcgccgc tactgcgcgc gcgctcgacg aggagatcgt cgccaggttg
     3001 ggtgccgagg aagtcggtac caggctcgag gccatcaacg cgctcgttgc atccggcgac
     3061 gaagccgcgc tcgcgctgtt gcaggccatg caggatggcc aggtcaacct cacaccctcc
     3121 ggccgcgtgg tggtcgtccg gggcgacaag gccttcgatg cggccaccat ggcgccgatc
     3181 gagcccgaac ccgagggcct cgaaagcatc gtcgtcaaca acaggattcg cggcgcgctc
     3241 gacacggcca tcggcgcgct caagctgctg tcgcccgatc gcgcggtgcg gcttgcatcg
     3301 gcgaaggcga tcgaaacgac cagccaggtg gcactgctgc cgctgatcga gaaggcgatc
     3361 tcccgggaga gcgatcctgg gatcaggaaa tcgctgcaac aaaccaaggc ggcgctggac
     3421 ctgaaaagca gcgatcccgg aacccgattg gccgcggtca gcacgctggc cgagagcaat
     3481 caggcgcagg tcaagacccg gctgctggcg ctgctggaaa aagacgcttc cggaagtttc
     3541 gtcgagcccg acgcgcaagt gcgtgccgcg gcagaacggt cgatcgattc gatccagagg
     3601 cggctgctga tcagcgacta ccttgcccgc ctgttcaccg gcctgtcgct gggcagcatt
     3661 ctcctgctgg tggcgctggg cctggcgatc acctacggcc tgctcggcgt catcaacatg
     3721 gcgcacggcg agatgctgat gatcggcgcg tatgcgacct tcgtcacaca gcagttgttc
     3781 aaatcctatg cgccggggct gttcgattgg tacctggtcg cggccatccc ggtggcgttc
     3841 gctgcctccg cgctggtcgg catggcgctg gagcgcagcg tgattcgctg gctttacgga
     3901 cggccgctgg aaacgctgct cgcgacctgg ggcatcagcc tgttcctgat tcaattggtg
     3961 cgccagatct tcggcgcgca gaacgtcgaa gtggcgaacc cctcgtggat gtcgggcggg
     4021 gtggaactta ccaacatggt gttgccgtac aaccgcattg ctatcatcgc cttctcgatg
     4081 ttcgtgctgt tgatgatgtg gctgatgctg tcgcgcaccc ggctcgggct gttcgttcgc
     4141 ggcgtgacgc agaaccgcat tatggcttcc tgcgttgggg tgcccaccga gcgcgtcgac
     4201 ttgctggcgt tcgggctggg ttcgggcgtg gccggccttg ccggctgcgc cctgtcgcag
     4261 atcggcaatg tcggacccga tctcgggcag agttacatca tcgattcgtt catggtggtg
     4321 gtgcttggcg gcgttggcca gctcgccggc accgtctacg ccgcgctcgg cctgggtgtg
     4381 ctgtcgaagt ttctggagcc gcacatcggc gcggtgctca ccaagatcct ggtgctggtg
     4441 ctgatcatcg ccttcatcca gaagcgcccg caggggctct tcgcgttgaa ggggcgcgcc
     4501 gttgaagctt gaagaactgg agatgcgata cgtcccgcgt atcgaaccgc tgttcagcgg
     4561 gccgggctgg atggcactgg gcgccttcgc cctggtgacg ctggtagtgt tgccgctgct
     4621 gtacctgctg cctgcagcgg atagtgcgct gcacgtgtcg tcgtatacca tcacgcttgc
     4681 cggcaagatc atgtgctact gcatcgtcgc catggcgatg aacctgatct ggggctatac
     4741 ggggattctc tcgctcggac acggggtgtt cttcgcgctg ggcggttacg cgttcggcat
     4801 gtacctgatg cgccagatcg ggcgcgaggg gcagtaccag tcggacctgc cggacttcat
     4861 ggtgtttctg gactggaagg ctttcccgtg gttctggacc gggtcggagc actttctctg
     4921 gagcgcgaca ctggtgatcc tggcgcccgg cctgctggcg ctggcattcg gctacttcgc
     4981 cttccgctca cgcatcaagg gtgtgtattt ttccatcatc acccaggcga tgaccttcgc
     5041 tttcatgctg ctgttcttcc gcaacgacac cggcttcggt ggcaataacg gcttcaccga
     5101 cttcaagcgc gtgctgggat ttccgatcac tgcgccttcc acgcgcgggg cgattttcgc
     5161 catgaccgcg ctggcattga tcggcgtggt cttcctgacg cgctacctga tccgctcgaa
     5221 ggtcggccgc gtgctgaccg ccatccgcga ctcggaaagc cgtctgatgt tctccggcta
     5281 caacccgctc ggctacaagc tgttcgtgtg gacgctgtcg gccgtgctgt gcggcatcgc
     5341 tggagcgctg tacgtgccgc aagtcggcat catcaatccg agcgaaatgt cccccgccaa
     5401 ttcgatcgag attgcggtgt gggtcgcggt cggcggccgc ggctcgctga tcgggccgct
     5461 gctgggcgcg gcagtggtga acggcgcgaa gagcttcttc acccagactt tcccggagta
     5521 ctggctgttc ttcctcgggt tgctgttcat cctggttacg ctgttcctgc ctaggggcgt
     5581 cattggtctg ctgagcccgg ccaggtggag acgcacatga acacgatctc cgaagaaatc
     5641 agcgtcagcg gcaacacttc cggactcagc cacgtcctgc aaggcggggt ggacatcacc
     5701 cacggcccga ttctttacct cgacgatatc accgtgagct tcgatggctt caaggcactc
     5761 aacaagctca cgctcacggt cgaggccggc gagctgcgct gcgtgatcgg tccgaacggc
     5821 gccggcaaaa ccaccatgat ggacgtgatc accggcaaaa cccgtcccga cgaaggctcc
     5881 gcgtacttcg gccagaccat cgacctggcg cgcctgaccg aaccggagat cgcgcacgcg
     5941 ggcattggcc gcaaattcca gaaaccgacc gtattcgaga accacacggt gttcgagaac
     6001 ctcgagctgg cgatgaagac cgacaagcgt gtttgggcga gcttgcgtgc acgcctgagc
     6061 gccgaacagc tcgatcgcat atgcaaaacc ctggaactga tcaagctgca gggcgaagcc
     6121 ggtcgcctcg ccgggttgct gtcccacggc cagaagcaat ggctggagat cggcatgctg
     6181 ctgatgcagg aaccgcgcct gctgctgctc gacgaaccgg tcgccggcat gaccgacgaa
     6241 gaaaccgaac gcaccgctga actgttcaag tcgctggccg gtgaacgctc tgttgtggtg
     6301 gtcgagcacg acatggagtt catcggctcc atcgccagcg tcgtcaccgt gttgcacgaa
     6361 ggcgccgtgc tcgccgaggg cacgatggac gccgtcaaga acgacgagcg cgtcatcgaa
     6421 gtgtatttgg gtcgttgacg cggggacaaa cggaaaaacg gaaaatctca ccacgaagac
     6481 acgaagaaca cgaaggtgtc atgacgaaat tcaggataag tgctgaagtg atacgagaga
     6541 aaaatgggat ggaagtgcga tgggtatcct tatgacaacg ggcgagtccg taactgcaat
     6601 ttgagaaagg tgaaggcagt tgctttcctg cattgttcgt ctttgtgttt cttcgagttc
     6661 ttcgtgtctt cgtggtcata ggtttcattc tcaggaatct gaagaatgtt aaaggttgag
     6721 aaactcaatc agttctacgg cagcagccag acgctgcgca acgtgagctt cgaagtgccc
     6781 acgggggcgg tgaccg
//
LOCUS       DASQOP010000301        35868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1961816, whole genome shotgun sequence.
ACCESSION   DASQOP010000301 DASQOP010000000
VERSION     DASQOP010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 35868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35868
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1961816"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..1233)
                     /locus_tag="VE030_11920"
     CDS             complement(49..1233)
                     /locus_tag="VE030_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZF24160.1"
                     /translation="MDLSTAQILPTDAERATLIGRAWVPGRMGGPSPVVIAGGRVHDL
                     ARVAPTVSELLNSRDPVKMARESVKTGWAREIGEVADLISNSHADRRDPRKPYLLAPC
                     DLQALRACGVTFIASMLERVIEEHAKGDPLKAEEIRLSIGAEIGAELKSVKPGSAEAM
                     RLKESLIRRNLWSQYLEVGIGPDAEVFNKSQPLSAVGVGAEIGIRADSSWNNPEPEVV
                     LAINRAGAIVGAALGNDVNLRDFEGRSALLLGKAKDNNASCAIGPFIRLFDERYGIDD
                     LRKTRIHIEVAGPEGYRLSGESHLANISRDPLDLASQAVNRTHQYPDGTMLFLGTQFA
                     PTKDRGEKGKGFTHKLGDIVSIRADALGMLVNRVNHSDKVVPWTFGVNDLMRNLAGRG
                     LL"
     gene            complement(1248..1796)
                     /locus_tag="VE030_11925"
     CDS             complement(1248..1796)
                     /locus_tag="VE030_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24161.1"
                     /translation="MTFHVVLLFLSFAAAQALAHSEGETSGPVLLDCEHPPQLLVKML
                     PEALAKVASVECNPSAQTIVARDGWSWRYPGSFFDRPIIPAYAPMDSRQTGGPRYFTE
                     FKATELSAADARKRHEMFAKDLPTYRETSPPMRLVKLVAHNDLGHEMDAYFGFRSDGG
                     GWVVLCAPECAPEYLFLMQKID"
     gene            1957..3375
                     /locus_tag="VE030_11930"
     CDS             1957..3375
                     /locus_tag="VE030_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HZF24162.1"
                     /translation="MLDHPLVFLDLETTGATAHCDRITEIGLVEVDCGRHVGEWSSLV
                     NPEMRIPPSIQTLTGITDAMVANAPTFADLAESLYDRLEGKLLVAHNARFDYGFLKHE
                     FRRLGLRYQPRVLCTVKLSRRLYPQHRHHNLDSLIERHGLPCDVRHRALADARVLWEF
                     TRQIHLDLGPKVVRAAVEDLLKKPALPPGLPANVLDDISEGPGVYVFYGENDIPLYVG
                     KSIALRSRVMSHFNSDHRVAKDMRISQEITRVEWIETAGELGALIKEAQLVKRLAPIH
                     NRRLRRNNDPCVFRWDAVNGPKTPQLLSAADIDFSGLDKLYGMFRSRRVALNALREIA
                     DEHELCHIGIGLEKGKGPCFAYQLKRCRGACVGEESAISHSMRVTEALHSLRMQAWPF
                     PGRIGIREHDPHGRRTEIHVLDCWCYLGTLNSQMDLFDMLQTRAEPSFDLDTYKILTR
                     YFKSRPRNVEIVHLPESVRREG"
     gene            complement(3445..4230)
                     /gene="pcaD"
                     /locus_tag="VE030_11935"
     CDS             complement(3445..4230)
                     /gene="pcaD"
                     /locus_tag="VE030_11935"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR02427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HZF24163.1"
                     /translation="MKVNSNGIGINYAVEGKGPWVVLSHSLACNHSMWDEQADALKRH
                     YRVLRFDTRGHGGSDAPDGAYTLDMLAEDLLGLLDSLSIEETHFVGLSMGGMIGMTFA
                     LAHPDRFRSLVLCDTSSRIPAEATPVWEGRIKTAAEQGMEPLVEPTLQRWFTEAFYKS
                     NKAMMKRVGQMIRTTRPAGYIGCCHAIPNINLTDRLGAISCPVQVIVGEQDVGTPVAM
                     SRAIHDAIPGSKLVIIPSASHLSNLEQPELFNKALLSFLSKHG"
     gene            4372..5373
                     /gene="araD1"
                     /locus_tag="VE030_11940"
     CDS             4372..5373
                     /gene="araD1"
                     /locus_tag="VE030_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraD1 family protein"
                     /protein_id="HZF24164.1"
                     /translation="MRLVQFKDRSGARKVAATSEDGQTLRVLAGVTRTYDLALAAARN
                     KTPLEAAAKDKIGAETASYDEVVNDHRLLPPLDHPDPYHCLISGTGLSHLGSAAARDS
                     MHVKLKQAETEMTDSMKMFKWGVDGGKPPPGQIGVAPEWFFKGDGRCVVAPEQPLELP
                     SYALDGGEEVEVVGLYMVGDKGAVLRVGFSLGNEYADHVMERQNYLYLAHSKLRPCSY
                     GPELLVGELPKSVSGRARLMRDGREIWAEDWLSGEDNMCHSIANLEHHHFKYRDFRQP
                     GDVHVHFFGAATGSFIKKIETRIGDVFEISSPQFGRPLRNPLGPAQDTGKLVTVRAL"
     gene            5370..5498
                     /locus_tag="VE030_11945"
     CDS             5370..5498
                     /locus_tag="VE030_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24165.1"
                     /translation="MSDVQGDIGAVSAAQKAFDSVERTGLHRIAAGKVVRLASRRH"
     gene            complement(5723..6538)
                     /gene="folE2"
                     /locus_tag="VE030_11950"
     CDS             complement(5723..6538)
                     /gene="folE2"
                     /locus_tag="VE030_11950"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004552018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HZF24166.1"
                     /translation="MNSPEGFSIPDVQSSADTRRLAIDKVGIKAIRHPVRVADRSDGV
                     QHTIATFNMYVGLPHNFKGTHMSRFVEILNSHEREISVESFEAMLRDMVKRLEAEFGH
                     IEMTFPYFINKAAPVSGVKSLMDYEVTFVGEVFRNQYVFTMKVVVPVTSLCPCSKRIS
                     EYGAHNQRSHVTISARTNTHVWIEDLVRIAEDQASCELYGLLKRPDEKYVTERAYDNP
                     KFVEDMVRDIATVLNQDKRIDAYVVESENFESIHNHSAYALIEKDKSKPATKN"
     gene            complement(6601..8445)
                     /gene="dxs"
                     /locus_tag="VE030_11955"
     CDS             complement(6601..8445)
                     /gene="dxs"
                     /locus_tag="VE030_11955"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HZF24167.1"
                     /translation="MYELLEKISNPSDVRALERKQLAALARELRDYLIHSVANTGGHL
                     SSNLGTVELTVALHYVFNTPHDRLVWDVGHQTYAHKILTGRRERMSTLRQYGGVAGFP
                     RRIESEYDTFGTAHSSTSISAALGMAVAARMKGEDRRVVAIIGDGAMTAGMAFEALNN
                     AGAMDANLLVVLNDNDMSISENVGALNNYLAKLMSGRFYAAAWKTSEKVLGVVPPMLE
                     LAKRAEEHVKGMVTPGTLFEEFGFNYIGPIDGHNLDVLLSTLSNLKKLEGPQFLHIVT
                     RKGKGYEPAEKDPILYHGVTKFDPDAGITPKSGGKPTYTQIFGDWLCDTAALDSRLIG
                     ITPAMREGSGLVRFSQEYPDRYFDVGIAEQHALTFAAGLACEGLKPVVAIYSTFLQRA
                     YDQLIHDICIQNLPVLFGIDRAGLVGADGPTHAGSFDLTYLRCLPNMVVMAPSDENEC
                     RQMLHTGFQIDGPAAVRYPRGSGPGVPVQKDLTALTIGKGEVRRRGQRVAILSFGALL
                     KPCVEAAEKLDATVANMRFVKPLDEELIRELAISHEWLVTVEENTILGGAGSAVLEAL
                     EKKGIQKKVLQLGLPDRFVDHGDPARLLKECGLDAEGIHASIAIKLAE"
     gene            complement(8460..9329)
                     /locus_tag="VE030_11960"
     CDS             complement(8460..9329)
                     /locus_tag="VE030_11960"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016355303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HZF24168.1"
                     /translation="MLDRQDRIEKTLNALLPAAELAPARLHAAMRYAVLGGGKRVRPL
                     LAYGAGELSGADPQRVDRAAAAVEMIHAYSLVHDDLPCMDDDVLRRGKPTVHLEFDEA
                     TALLVGDSLHSLAFQVISEQSMADNAQHQVEMIEFLARACGSRGMAGGQAIDLEATGK
                     TLAVTELEFMHVHKTGALIRASVALGARCGDAMSQAQFKELDRFGKFIGLAFQVVDDV
                     LDAQSSTATLGKTAGKDAKNNKPTYVSALGLEGARKFAAELRDDALRALDALPQNGGQ
                     LRPLADFIVLRRF"
     gene            complement(9343..9582)
                     /locus_tag="VE030_11965"
     CDS             complement(9343..9582)
                     /locus_tag="VE030_11965"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HZF24169.1"
                     /translation="MTKPKSQKPQSFEQALAEIESIVAAMEAGQLPLEQSLAAYKRGA
                     ELLQYCQAQLQEAQQQVKVLEAGTLQNFSGDDGKE"
     gene            9813..10916
                     /locus_tag="VE030_11970"
     CDS             9813..10916
                     /locus_tag="VE030_11970"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZF24170.1"
                     /translation="MTDLANVSALTRTPAQPPVEWYLDPRIARIEHKVLFQEGPGYVG
                     HKLMVPEVGDYRTLEWMGHAKALVHNQNGVELLSNVCRHRQSIILKGHGRTHNIVCPL
                     HRWTYDTSGRLLGAPHFPGNPCLDLETTPLAQWNGLLFAGPRNPNLDLSKLGVLADLD
                     FSGYVLDRVMIEEYPVNWKTFIEVYLEDYHIGPFHPGLGQFVDAENLKWEFGEWYSVQ
                     TVGINQALGRPGTDAYKKWHEAVLRYNNGEMPKQGAIWLTYYPNIMVEWYPNVLAVSS
                     IVPRGPEHCSNVVEFYYPEDIVLFEREFVEAEQRAYIETAREDEEICLRMTEGRRALY
                     QQGRNEFGPYQSPMEDGMIHFHEFLRNHIEPHL"
     gene            11019..11858
                     /locus_tag="VE030_11975"
     CDS             11019..11858
                     /locus_tag="VE030_11975"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014724694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HZF24171.1"
                     /translation="MKYTTLISVAELANHLGNAELVIFDCRHELTNPEYGPNAYAESH
                     IPGARFAHVDRDLAGRLAGKNGRHPLPDPNAFVAWLGRMGVGSGSQVVGYDHAGGTYA
                     ARLWWMLRWVGHRKVAVVDGGWQAWAAAGNPVTKELPTPTAAKFSGKPDRSWVDAAAV
                     TKRLNSKEMILIDARSNDRFHGRNETIDPVVGHIPGALNRFFKDNLNTQGFFKSPQEL
                     RADFNSLVGDASPEKVVHQCGSGVSACHNLLAMEIAGIKGTRLYPGSWSEWIADPSRP
                     VAS"
     gene            11877..12287
                     /locus_tag="VE030_11980"
     CDS             11877..12287
                     /locus_tag="VE030_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015603733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HZF24172.1"
                     /translation="MSGLADVVLLVHALFVVFVVGGLALIWIGALCGWRIVRNFPFRV
                     LHLAAILFVCAEALIGVACPLTAWEDQLRGGPAQSDFVARWLHRILYYDFPGWVFLTL
                     YLLFALAVAGSFLLIRPSRDHDRNKNGRQSRPSG"
     gene            complement(12303..13574)
                     /locus_tag="VE030_11985"
     CDS             complement(12303..13574)
                     /locus_tag="VE030_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZF24173.1"
                     /translation="MTRRDFVKWLSASAALSGSIGCATATTSKTVGRVVVIGGGYAGA
                     TAAKYVRMWGPDIEVTLVERDAEFVSCPLSNRVLAGVIQLKDLTRDYGKLQAKYGVKL
                     IRDEAKAIDPVAQEVRLGKGGTLKYDRLIVAPGVDILYNEVPGLDSPEAQARVLHAWK
                     AGPQTVALRKQLEAMADGGVYAMTIPKAPYRCPPGPYERACQVAWYFKTSKPKSKVLI
                     LDANEDVVSKKGLFTKAWSERYAGIIEYRPNSELTGVDAATMTAKLAFEDVKADVLNV
                     VPPQRCGKIAADAGLITANNRWCGVDFLTYESSAVKNVHVLGDAIMAAPQMPKSGHMA
                     NQHAKVCAAAVVALLHGEAVNSQPLVTNTCYSFVSDKEVIHVASVHAYVPEKKTMEIV
                     PGGSGVSAAASELEGQYAEAWGRNIWSDMLG"
     gene            complement(13606..13917)
                     /locus_tag="VE030_11990"
     CDS             complement(13606..13917)
                     /locus_tag="VE030_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZF24174.1"
                     /translation="MIRAGITYAALLLAGASIPALAVDNNINGRNWAATCTGCHGTNG
                     HSEGGMPLLAGLDKAYIVNAMKEFKAGTRPATVMHQHAKGYSDEQIERIAEFFAAQPL
                     K"
     gene            complement(14094..16358)
                     /gene="clpA"
                     /locus_tag="VE030_11995"
     CDS             complement(14094..16358)
                     /gene="clpA"
                     /locus_tag="VE030_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HZF24175.1"
                     /translation="MIAQELEVSLHMAFMEARQKRHEFITVEHLLLAMLDNPSAAEVL
                     RACAANVEELRKLLNDFINEHTPVVSGEEVDTQPTLGFQRVIQRAILHVQSSGKKEVT
                     GANVLVAIFGEKDSHAVYFLHQKGVTRLDVVNFISHGITKVPQAAPAKQQPEADADSE
                     QEQSSGGALESYTMNLNALAISGKIDPLIGRERELERVIQTLCRRRKNNPLLVGEAGV
                     GKTAIAEGLARRIIEGSVPEILSRCQVYALDMGALLAGTKYRGDFEQRLKAVLKQLTD
                     NPNAILFIDEIHTLIGAGAASGGTLDASNLLKPALSSGALKCIGATTYTEFRGIFEKD
                     HALSRRFQKIDVTEPSVAETVEILRGLKTRFEAHHGVKYTASALTSAAELSARFINDR
                     HLPDKAIDVIDEAGAAQRILPKSKQKRVISKHEIEEIIAKIARVPAQSVSSTDRSKLQ
                     NLDRDLKAVVFGQEKAIDALAASIKMSRSGLGNPQKPIGCFLFSGPTGVGKTEVARQL
                     AYTMGVELIRFDMSEYMERHAVSRLIGAPPGYVGFDQGGLLTEAITKHPYSVLLLDEI
                     EKAHPDIFNILLQVMDHGTLTDNNGRKADFRNIVIVMTTNAGASELSKTSMGFTQQTV
                     AGDEMAEIKRMFTPEFRNRLDAAISFSALSHDIIIKVVDKFLMQLEEQLHEKKVEVAF
                     TDALKAYLAKSGFDPLMGARPMSRLIQDTIRTALADELLFGRLASGGKVTVDVDAEGK
                     TRLLFDEAKEESLA"
     gene            complement(16355..16663)
                     /gene="clpS"
                     /locus_tag="VE030_12000"
     CDS             complement(16355..16663)
                     /gene="clpS"
                     /locus_tag="VE030_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010807118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HZF24176.1"
                     /translation="MASGTRDSTLLEAEKSKVKPPPLFKVLLLNDDYTPMDFVVSVLQ
                     TFFSMTREQATQIMLKVHREGMGVCGVYPKDVAATKVEQVIAFSRQHQHPLQCVMEET
                     "
     gene            16960..17163
                     /locus_tag="VE030_12005"
     CDS             16960..17163
                     /locus_tag="VE030_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HZF24177.1"
                     /translation="MATGTVKWFNDAKGYGFITPDDGSEDLFAHFSAIQMNGFKTLKE
                     GQKVSFEVTQGPKGKQASNIQAA"
     gene            complement(17256..18500)
                     /gene="icd"
                     /locus_tag="VE030_12010"
     CDS             complement(17256..18500)
                     /gene="icd"
                     /locus_tag="VE030_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HZF24178.1"
                     /translation="MYQHIKVPSTGQKITVNKDYMLNVPANPIIPFIEGDGTGVDITP
                     VMRRVVDSAVQVAYESKRKVVWMEMYAGEKSCTVYGENVWLPEETLQASKEFVVSIKG
                     PLTTPVGGGIRSINVALRQQLDLYVCLRPVRYFKGVPSPLKQPEKTDMVIFRENSEDI
                     YAGIEWAAESEDSRKVIDFLIKEMGVKKIRFPRTSGIGIKPVSREGTERLVRKALQYA
                     IANKRRSVTLVHKGNIMKFTEGAFRDWGYALAKREFGAALLDGGPWMKLPNGMVVKDA
                     IADAFLQQILLRPDEYDVIATLNLNGDYISDALAAQVGGIGIAPGANTSDSVAMFEAT
                     HGTAPKYAGKDYVNPGSLILSAEMMLRHMGWVEAADLIVRSMEAAIRDKVVTYDFARL
                     MTGATQVSCSGFGNAMIERMKD"
     gene            complement(18689..18970)
                     /locus_tag="VE030_12015"
     CDS             complement(18689..18970)
                     /locus_tag="VE030_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24179.1"
                     /translation="MLELGLESALRQRLLAQRWCRRQYIRIFMRRNNKAELEFHIKKY
                     KLMNFHGARFCNAKFGFKDFADSSSLAYCRGWMRVATRTTPDPSMRGAL"
     gene            19174..20451
                     /gene="aceA"
                     /locus_tag="VE030_12020"
     CDS             19174..20451
                     /gene="aceA"
                     /locus_tag="VE030_12020"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HZF24180.1"
                     /translation="MTRDLEITRIKKDWSDHPRWKGVQRTYSAEDVYRLRGTLAVEYT
                     LARRGAEKLWQLLRDEPFVNALGALTGNQALQQVKAGLKAIYLSGWQVAADANLSGEM
                     YPDQSLYPANSVPAVVRRINNTFIRADQIHHAEGNDDIDWFAPIVADAEAGFGGVLNA
                     FELMKSMVEAGAGGVHFEDQLASAKKCGHMGGKVLVPTQEAVQKLAAARLASDIMGVP
                     TVLFARTDAEAANLLTSDFDENDKPFCTGERTAEGYYRVINGIQQAISRGLAYAPFAD
                     LIWCETGKPDLAFARQFAEAIHKQYPGKLLAYNCSPSFNWKKNLDDASIARFQRELGA
                     MGYKFQFITLAGFHSLNYSMFELARGYATENMSAYVRLQQAEFAAEAHGYTATKHQRE
                     VGTGYFDDVTQAVQAGRSSVTALTGSTEEEQFV"
     gene            20579..22195
                     /gene="aceB"
                     /locus_tag="VE030_12025"
     CDS             20579..22195
                     /gene="aceB"
                     /locus_tag="VE030_12025"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HZF24181.1"
                     /translation="MTQPNIPSGMKVLGHITPEYAQILTGGALAFLAKLHRNFNTRRL
                     ELLERRALRQKELDSGKLPDFLAETKHVREADWQVAPVPRDLQDRRVEITGPTDRKMV
                     INALNSAANMFMADFEDSSTPTWDNQLQGQINVRDALTRSISFTSPEGKQYRLADKTA
                     TLLVRPRGWHLCEKHVVVDGQPMSGSLFDFGLYFFHNAKARIKESSGVYLYLPKMESH
                     LEARLWNDAFEFAQKELGVPQGTVKATVLIETILATFEMEEILYELREHSAGLNCGRW
                     DYIFSCIKKFRNRKDFILADRHLVTMVSPFMRAYSLLLIKTCHKRGTFAMGGMAAQIP
                     IKNDPTANEAALAKVRADKEREASDGHDGTWVAHPGLVAIAKEAFDKVMKTPNQIHKK
                     RDDVNVSAKDLLNFQPRGPVTEAGLCTNINIGIQYLGAWLAGAGCVPIFNLMEDAATA
                     EISRAQIWHWIRSPLGILDDGRKVTRELFRDLVPQELEKVRQLLGDKGFATGKYPEGA
                     RMFEELTLAEDFVEFLTLPAYDYLITHEHR"
     gene            22229..22786
                     /locus_tag="VE030_12030"
     CDS             22229..22786
                     /locus_tag="VE030_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HZF24182.1"
                     /translation="MPRVLLFNKPCGVLCQFSPSAGKRTLKDFIAVADVYPAGRLDAD
                     SEGLVVLTDDGKLQHAISDPKMKLPKTYWVQVDGEPDEVALRRLRSGVDLDDFLTRPA
                     GARRIDQPSGLWERIPPIRVRKSIPTSWIELELHEGKNRQVRRMTAAAGYPTLRLIRS
                     RIGPWTLDGILPGQFRLVDHQQLRI"
     gene            22906..23082
                     /locus_tag="VE030_12035"
     CDS             22906..23082
                     /locus_tag="VE030_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24183.1"
                     /translation="MKKLFAALIAALFAAVTFSAVAADTAVNPADSAKPAKSAKKHHK
                     KAAKKVEETAPAKK"
     gene            23305..23937
                     /locus_tag="VE030_12040"
     CDS             23305..23937
                     /locus_tag="VE030_12040"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HZF24184.1"
                     /translation="MSEWADILKLTVTLLAIVDPIASIPVFLTTTDGESNMQRARIAR
                     IVAITVFCVLGAAALIGTEILHFFGISIPSFLIGGGIVLMMMSISMLQAKESRIRQTP
                     DEAEEAAEKHAVAVVPLGIPLLAGPGAISTMIIATHQSPSFIYHLKLLIPALIIAVAV
                     WAALIAATRISKRLGKTGMNIITRVMGLIIAAIAVEYVYRGLIELFPKLI"
     gene            23938..24393
                     /locus_tag="VE030_12045"
     CDS             23938..24393
                     /locus_tag="VE030_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZF24185.1"
                     /translation="MTAKLWKPNVTVAAIIEREGRFLLVEEETDDGLLLNQPAGHLEA
                     NESLVEAAAREALEETAYEFTPEYLVGVYRWRHPDRNVTYLRFAFAGRLGTHHPERAL
                     DTGIVRALWMTAAEVRASRSRHRSPLVIRCVEDYQRGARAPLDLLVHLD"
     gene            24407..25504
                     /gene="mnmA"
                     /locus_tag="VE030_12050"
     CDS             24407..25504
                     /gene="mnmA"
                     /locus_tag="VE030_12050"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008351536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HZF24186.1"
                     /translation="MQKRRVVVGMSGGVDSSVAAWLLKEQGFDVVGLFMKNWEDDDDD
                     EFCTSRQDLIDAVSVADLIGIDIEAVNFSAEYKDRVFSNFLAEYQAGRTPNPDVLCNA
                     EIKFKAFLDHALQLGAEQIATGHYAQVRQVDGLFQLLKAEDGTKDQSYFLYRLDQAQL
                     SKTLFPLGAMYKRDVRELARKLGLPNHDKKDSTGICFIGERPFREFLRRYLPTQPGDM
                     VTPEGKTVGRHIGLMYYTIGQRQGLGIGGRKDGEGEPWYVAGKDMTANRLIVVQGHDH
                     PALLADALRATDLNWISDAEPHCHWVYGAKTRYRQKDAPCEVERVDRVHCEIKFAEAQ
                     WAITPGQSVVVYESKVCLGGGIIESPTAAGK"
     gene            complement(25538..26170)
                     /locus_tag="VE030_12055"
     CDS             complement(25538..26170)
                     /locus_tag="VE030_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012366171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HZF24187.1"
                     /translation="MTRKTAKRAAPGQRSGRELEALLKKVHACRVCEAHLPFGPRPVL
                     RASVTAQLLIVGQAPGRRVHETGIPWNDPSGERLRDWLQLDRERFYDQRHIAIIPTAF
                     CYPGTGAGGDLPPRPECAPLWHPQLLAHLPKLRLALLIGSYAQAYYLGDRRKSTLTDT
                     VRAWREYLPYCIPLPHPSPRNQMWLKRNPWFAGEVIPYLRRTVHQVLEGR"
     gene            26232..27605
                     /gene="purB"
                     /locus_tag="VE030_12060"
     CDS             26232..27605
                     /gene="purB"
                     /locus_tag="VE030_12060"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HZF24188.1"
                     /translation="MTHPTPLTALSPLDGRYRDKVAGLTHYFSEYGLIRYRVFVEIEW
                     LKALAAAKGLPEIKPFASETITRLDELAAKFSVSDGEAVKAIEARTNHDVKAIEYWLR
                     EKLSGNREIAGITQFIHFACTSEDVNNLCHALMLRDSRAQVLLPAIDALVDKLVSLAH
                     ANADLPMLSRTHGQPATPSTLGKELANVVHRLRRQRNRIGSVALTAKINGAVGNYNAH
                     LAAYPDFDWEQFCRSFVEMVGFEFNPYTTQIEPHDCIAELLDAYAGINTVLLDLDRDM
                     WGYISIGYFKQRIKEGEVGSSTMPHKVNPIDFENSEGNIGLANALLTHLASKLPVSRW
                     QRDLTDSTVLRNLGIALGYSLLAYDGCLRGLSKLEPDPKRIGADLEANWEVLAEPIQT
                     VMRRYGVEDAYEQLKALTRGKEGITRESLHQFIRTLAIPQPEKERLLRLTPATYTGLA
                     AQLAKRI"
     gene            complement(27616..28254)
                     /locus_tag="VE030_12065"
     CDS             complement(27616..28254)
                     /locus_tag="VE030_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HZF24189.1"
                     /translation="MDLIAGFLDLVVHLDQHLRALAQNYGNWIYAVLFLIVFLETGLV
                     VTPFLPGDSLLFVAGTIAAGGELHVHGLVMLLIMAAIIGDSVNYAVGRYLGPRVFRFE
                     DSRFFRRAYLERTHAFFERHGGKTIIIARFVPIIRTYAPFVAGIGAMRYRHFLLFNVT
                     GAMLWVASLTYAGYFFGNLPVVKDNLTLVIVGIIVLSLMPGIIEFWRARRAS"
     gene            complement(28270..29199)
                     /gene="secF"
                     /locus_tag="VE030_12070"
     CDS             complement(28270..29199)
                     /gene="secF"
                     /locus_tag="VE030_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HZF24190.1"
                     /translation="MEFFRIRKDIPFMRHALVFNVISLITFILAVFFLATKGLHLGVE
                     FTGGTVMEVNYGHAADVSKIREALNRINLSDATVQSFGSSQTALIRLPSKQGVTGAQL
                     SERVIKALREDDRTVELRRVDFVGSQVGKELVENGALALLLVSIGIVCYLWLRFEWKF
                     GVSAIVANLHDVVIILGFFAFFQWEFSLPVLAAVLAVLGYSVNESVVVFDRIRENFRK
                     MRKAGTPEIINNAITRTMSRTIITHGSTQLMVTSILVFGGETLHYFALALTIGILFGI
                     YSSVLVASPLVMWLGVTREDFIRPEKKKEEAVV"
     gene            complement(29238..31100)
                     /gene="secD"
                     /locus_tag="VE030_12075"
     CDS             complement(29238..31100)
                     /gene="secD"
                     /locus_tag="VE030_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HZF24191.1"
                     /translation="MNRYPLWKYVIIAVALVVGFLYTLPNFFGEVPAVQISPGKSTAK
                     IDEALRTRVEQALAQANIVPDGVFLETNSIKVRLGNTDTQLKAKDVLQSALGENYVVA
                     LNLLSSSPHWLTRINALPMYLGLDLRGGVHFLLQVDMAGALTKAADRYAADIRTTLRA
                     KEIRYSGISREGQTVQIRFRDAEARDKAAAEIQRQNPDLLLKPDDSGSEFLVTASLKP
                     EVRKRIQDFALQQNITTLRNRVNELGVAEPIVQQAGLDRVVVQLPGVQDTAKAKDILG
                     RTATLEVRMVDEEHSDSASLQAAVGGQVPFGDELLYDREGVPYLLKREVILTGDRISD
                     AQPGFDNQTGEPAVHINLDGRGATIFQQITRESIGKRMAMVLVEKGKGEVVTAPVIRS
                     EIPGGRVQISGRMTTTEARDVALLLRAGALAAPMEIVEERTVGPSLGADNIKRGFDST
                     LIGFTAIAIFMIVYYNVFGVISVLALSANLMLLVALLSMLQATLTLPGIAAIALTLGM
                     AIDSNVLINERIREELRNGTTPQAAISAGYERAWATILDSNITTLIAGIALFMFGSGP
                     VRGFAVVHCLGIMTSMFSAVVISRGIVNLAFGARRRLEKLPIGNTDWYRRQPAA"
     gene            complement(31156..31440)
                     /gene="yajC"
                     /locus_tag="VE030_12080"
     CDS             complement(31156..31440)
                     /gene="yajC"
                     /locus_tag="VE030_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HZF24192.1"
                     /translation="MDLFGLLPLILMFVLLYFLLIRPQAKRAKEHRSMLAGLQKGDEV
                     VTAGGALGKITRVGDNFVSVEIAPNIEIQVQKPSITTLLPKGTVKTAGQG"
     gene            complement(31615..32727)
                     /gene="tgt"
                     /locus_tag="VE030_12085"
     CDS             complement(31615..32727)
                     /gene="tgt"
                     /locus_tag="VE030_12085"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HZF24193.1"
                     /translation="MVKMRFEIIAGDGPARRGRLTLAHGTVETPAFMPVGTYGSVKAM
                     SPDDLRTVGAQVVLGNTFHLWLRPGLDVIGAHGGLRRFMGWDGPILTDSGGFQVFSLG
                     EMRKITEEGVRFRSPVNGDACFLSPEESMRIQRVLESDIAMVFDECTPYPADRSQAAE
                     SMQLSLRWAERSRRAHEGNGNALFGIVQGGMYEDLRDTSLEALTDIAFDGYAIGGLSV
                     GESKEDMQRILRHTAPRLPAGAPRYLMGVGTPADIVDAVASGIDMFDCVMPTRNARNG
                     WIFTRNGIIRLRNARYRDDTGPLDPACACYTCRNFSRAYLHHLQRINEILGAHLNTLH
                     NLFFYQDLMRGLREAISAGRLSEHVDGLRLGQAQPC"
     gene            complement(32721..33764)
                     /gene="queA"
                     /locus_tag="VE030_12090"
     CDS             complement(32721..33764)
                     /gene="queA"
                     /locus_tag="VE030_12090"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HZF24194.1"
                     /translation="MRAMRIEEFDYELPQDLIAQVPAAQRSASRLLHLDGATGNLADH
                     FFVDLPSLLHSGDLLIFNDTKVIKARLQGVKESGGKVEALIERIVSPRRALAMLRTSH
                     TARPGQRIRFGDLAHATMIERQGELFLLEFGDDVAGVLERLGEVPLPPYIERRADSSD
                     EARYQTVYARVPGAVAAPTAGLHFDEQLLRRLREIDVRFAYVTLHVGAGTFQPVRVEN
                     VEQHVMHGELYEISAETAQAVAETRAGGGRITAVGTTSLRALESAAQSGKMISGGGET
                     RLFIRPGYGFKVVDRLLTNFHLPRSTLLMLVCAFAGTEFIRRAYRHAVDCRYRFFSYG
                     DAMLIDKAAQSRS"
     gene            33879..33965
                     /locus_tag="VE030_12095"
     tRNA            33879..33965
                     /locus_tag="VE030_12095"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33913..33915,aa:Leu,seq:caa)
     gene            34042..35022
                     /locus_tag="VE030_12100"
     CDS             34042..35022
                     /locus_tag="VE030_12100"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HZF24195.1"
                     /translation="MKLLIKKTDNDGRLALITDFLTTSWIIEVADSSNREELAHKILD
                     ADAMVSMNWPTDMPPAPRLQLLQLPGAGTDDIAFDSVPAKVAVCNVYEHEIGIAEYIL
                     SAMLQWVIGIPRMDAALRRGQWYGSHLSGPRHDELYGKTVGIIGYGRIGREVARRARA
                     FSMRVLACSRTPRSSDGFVERVDGMDRLPAVLSASDFVVLALPLEPSTAGVIGTQQLA
                     RMKPGTVIINVARGALIDERALFEACREKRIGGAVIDTWFKYPAEGHEVAEPSNLPFR
                     ELENVIMTPHASGWTEGLRPRRCRMIAGNLDRLARGEPLINVVKQPAGSR"
     gene            35062..35850
                     /locus_tag="VE030_12105"
     CDS             35062..35850
                     /locus_tag="VE030_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZF24196.1"
                     /translation="MDNWAAEQAARIIWSTWQANDRVDGLPADCRPSSRQEGYKVQSE
                     VARLSGQALFGWKIAATSQAGQKHIGVDGPLAGRLLESRTFQTGASVPMVNNLMKVAE
                     AEFAFRMALALPPRASAYSMQEVLDAVESLHPAIEIPDSRYREFAKVGAAQLIADNAC
                     AGYFVLGPATAAGWRPHDLAEHLASAWVNGKLAREGKGANVLGDPRLALTWIVNELSV
                     VGETLRAGHVVTTGTCLVPVEIAPGDHVVADFGLFGRVEARLAP"
BASE COUNT         7080 a        11121 c        10598 g         7069 t
ORIGIN      
        1 ctcggcagcc agcactgatt ctttccgcag tcccctcgcc cctcgttcct acaacaggcc
       61 tcttcccgcc agattgcgca tcaggtcgtt gacgccgaag gtccagggca caaccttgtc
      121 gctatggttg acgcggttca ccagcatgcc gagcgcatcg gcccggatgc tcacgatatc
      181 gccgagcttg tgagtgaatc ccttgccctt ctcgccgcgg tccttggtag gggcgaactg
      241 ggtgccgagg aacagcatcg tgccgtccgg gtactgatgc gtccggttca ccgcctgcga
      301 agcgaggtcc agaggatcgc ggctgatgtt ggcgagatgg ctttcgccgc tgagccggta
      361 gccctccggt cccgctacct cgatatggat gcgagtcttg cgcaggtcgt cgataccgta
      421 gcgctcgtcg aacaggcgta tgaacggccc gatagcgcaa ctggcattgt tgtctttggc
      481 cttgccgagc agcagggcgc tgcgcccctc gaagtcgcgc agattgacgt cgttgccgag
      541 cgcagccccg actatcgcgc cggcacggtt gatcgccagc accacttccg gctcgggatt
      601 gttccaggac gaatcggccc gaatgccgat ctccgcgcca acgccgaccg ccgacaaggg
      661 ctgcgacttg ttgaacactt cggcgtcggg gccgataccg acctcgaggt actgcgacca
      721 caggttgcgc ctgatcagcg attccttgag gcgcattgct tccgcggagc cgggcttgac
      781 cgatttcaac tcggcgccaa tctccgcgcc gatgctgagg cgtatctcct cggctttcag
      841 cggatcgccc ttggcgtgct cttcgatcac gcgttcgagc atgctggcga tgaaggtcac
      901 gccgcaggcg cgcaatgcct gcagatcgca gggggcgagc agatagggct tcctcggatc
      961 gcgccggtcg gcatgcgaat tggaaatcag gtcggcaacc tcgccgatct ctctggccca
     1021 tcccgttttc accgattccc gcgccatctt caccgggtcg cggctgttga gcagttcgct
     1081 cacggttggg gcaacgcgcg ccagatcgtg aaccctgccg ccggcgatca cgacagggct
     1141 gggccctccc atgcgtccag gtacccatgc gcggccgatc agcgtcgccc gctctgcatc
     1201 cgttggaaga atctgcgctg tcgaaagatc catgtgcccg cccctctcta atcgatcttc
     1261 tgcatcagga acaggtactc gggcgcgcat tcgggcgcgc acaacacgac ccagcctccg
     1321 ccgtcggagc gaaacccgaa gtaggcgtcc atttcatgac cgaggtcgtt atgcgcgacc
     1381 aacttcacca agcgcatcgg cggagaagtt tcccggtaag tcggcagatc cttcgcaaac
     1441 atctcgtgac gcttccttgc gtcggccgca ctgagttcgg tcgccttgaa ttcggtgaaa
     1501 taccgcgggc ctcccgtttg gcgggaatcc atgggcgcat aggcaggtat gatcggccgg
     1561 tcgaagaagc tgccgggata acgccagctc cagccgtcgc gggccacgat ggtctgggca
     1621 gagggattac actcgacgct tgccactttc gcgagcgctt ccggcagcat ttttaccagc
     1681 aattgcggag gatgttcgca gtccagcagc accggaccgc tggtctcgcc ttcggaatgc
     1741 gccagagcct gagcagcggc gaaagacagg aataacagga caacatgaaa cgtcacggtc
     1801 tcctcctctt gttgtgccgg ggattatacc gtcgcgagta ttgctttccg cggacgctaa
     1861 ccgtgcatga aggtccaact ggcgaattcc atcaccgcgt gaattatccg aatctccatt
     1921 gtttcaatgg cttgattctc gcccgagagg ctggacatgc ttgaccatcc gctcgtattc
     1981 ctcgatctgg aaaccacggg ggcaacggcg cactgcgacc gcataaccga aatcggtttg
     2041 gtggaagtgg attgcgggcg ccatgtcggc gagtggagtt cgctggtcaa tccggaaatg
     2101 cgcatcccac cctcgattca gacgctcacc gggattaccg atgccatggt ggcgaacgcc
     2161 cccaccttcg ccgatctcgc cgagtcactc tatgaccggc tcgaaggtaa attgctggtg
     2221 gcccacaacg cccgcttcga ctacggtttc ctcaagcacg agtttcgccg tctcgggttg
     2281 cgctatcaac ccagggtgct gtgcaccgtc aagctgtcgc gccggctgta tccgcagcac
     2341 cgccaccaca acctcgacag cctgatagaa cggcatggcc tgccctgcga cgtccgccac
     2401 cgagcgctcg ccgatgcgcg tgtgctgtgg gaattcaccc gccagatcca tctcgacctc
     2461 gggcccaaag tggtccgtgc cgccgtggag gacttgctga aaaagccggc gctgccgccg
     2521 ggcctgccgg ccaacgttct cgacgacatt tccgaagggc ctggcgtgta cgtcttttac
     2581 ggcgagaacg acattccgtt gtacgtgggc aagagcatcg ccttgcgttc gcgggtgatg
     2641 tcgcacttca acagcgatca tcgcgtcgca aaggacatgc gcatctcaca ggaaatcacg
     2701 cgcgtcgagt ggatcgaaac tgcgggggaa ctgggcgcgt tgatcaagga agcgcagctg
     2761 gtcaaacggc tcgcgcccat ccacaaccgg cggctgcggc gcaacaacga tccgtgcgtc
     2821 ttccgctggg atgcggtgaa cggaccgaag acgccacagc tgctaagcgc cgcggacatc
     2881 gatttctccg gactggataa gctttacggc atgttccgct cgcgccgggt ggcgttgaac
     2941 gcgctgcggg aaatcgccga tgaacacgag ctgtgtcaca tcgggatcgg gctggagaag
     3001 ggcaaaggcc cctgttttgc ctaccagctc aagcgttgcc gcggtgcctg cgtcggcgag
     3061 gaaagcgcaa tctcccattc gatgcgcgtc acagaagcgc tgcattcgct gcgcatgcag
     3121 gcctggccgt ttccggggcg gatcggcatc cgcgagcacg atccgcacgg caggcgcact
     3181 gaaatccacg ttctcgactg ctggtgttac ctgggcacac tgaactcgca gatggatttg
     3241 ttcgacatgc tgcagacccg ggccgagcct tcgttcgatc tcgacacgta caagatcctc
     3301 acccgctact tcaagtccag gccgcgcaac gtcgagatcg tccacctgcc ggaaagcgta
     3361 aggcgtgagg ggtgaggcgc aaccgccgaa gcagaatcgg gttgttcctc agctctcaaa
     3421 tatgcacacg tgaatcgttt gacgctaacc gtgctttgag agaaagctga gcagtgcctt
     3481 gttgaataac tcgggctgtt ccaggttcga cagatgtgag gccgaaggga tgatcaccag
     3541 cttcgatccg gggattgcat cgtggatggc acgcgacatc gccaccggcg tgccgacgtc
     3601 ctgttcacca acgatgacct gcaccggaca ggagattgcg ccaagccggt cggtcaggtt
     3661 gatgttcggg atggcgtggc agcagccgat gtatcccgcc ggcctggtgg tgcgaatcat
     3721 ctgtcccacc cgcttcatca tcgccttgtt acttttgtag aaggcttccg tgaaccagcg
     3781 ctgcagcgtc ggttcgacca gcggctccat cccctgctct gcggcggtct tgatgcgccc
     3841 ctcccaaacc ggagtggctt ctgctggaat cctgctcgag gtatcgcaca gcaccaggct
     3901 gcgaaaacga tccggatgcg cgagcgcgaa agtcatgccg atcatgccgc ccattgaaag
     3961 tccgacgaaa tgcgtttcct cgatactgag gctgtcgaga aggcccagca ggtcttccgc
     4021 cagcatgtcc agggtgtagg cgccgtccgg tgcatcgctg ccgccgtgtc cgcgggtgtc
     4081 gaaacgcaac acgcgatagt gccgcttcaa cgcgtccgcc tgttcgtccc acatcgaatg
     4141 gttgcacgcg agcgagtggc tgagaacgac ccacggcccc ttgccttcga cggcgtaatt
     4201 gatcccgatg ccgttgctgt tgaccttcat gtctcctccc gtgtgccggc tgcagtatag
     4261 ggcggcgagc gcagcccttc aacgacccac agccgcggca gccgcagatt cgtcgtaaca
     4321 ttcgcatcgc aggtaaagca atcacaaaaa tcgggaacaa agggaggtat cttgagactc
     4381 gttcaattca aagaccgttc cggcgcccgc aaggtcgccg cgacatcgga ggacggccag
     4441 acgctgcgcg tactcgcggg cgtgacacgc acttacgacc tggcacttgc ggccgcgcgc
     4501 aacaaaacac cgctcgaagc cgcagcgaaa gacaagatcg gggcggaaac ggcaagctac
     4561 gacgaagtgg tcaacgatca ccgcttgctg ccaccgctgg accatcctga tccctaccac
     4621 tgcctgatct ccggtaccgg gctgtcgcac ctgggcagtg ccgccgcgcg tgactcgatg
     4681 cacgtcaagc tcaagcaggc ggaaaccgag atgaccgatt cgatgaaaat gttcaagtgg
     4741 ggcgtggacg gtggcaagcc gccgcccgga cagatcggcg ttgctccgga gtggttcttc
     4801 aagggcgacg gccgctgcgt agtcgcgccg gaacaaccgc tggaactgcc atcatatgcg
     4861 ctcgatggcg gcgaggaagt ggaagtcgtc ggcctgtaca tggtcgggga caagggcgcg
     4921 gtgctgaggg ttggcttttc actcggcaac gagtacgcgg atcacgtgat ggagcgccag
     4981 aactatctgt atctcgcgca ctcgaaattg cggccgtgca gctacgggcc ggagttgctg
     5041 gttggcgagt taccgaaatc cgtttccggc cgtgcacggc tgatgcgcga cggccgggag
     5101 atatgggccg aggactggct ctcgggcgaa gacaacatgt gccattcgat cgccaatctc
     5161 gagcatcatc atttcaaata ccgtgatttc cgtcagcccg gcgacgtgca tgtgcatttc
     5221 ttcggcgccg ccaccggcag cttcatcaag aaaatagaga ccaggatcgg tgacgtcttc
     5281 gagatcagtt cgccgcagtt cggcagaccg ctgcgcaacc cgctcggtcc ggcgcaggat
     5341 accggcaagc tggtaaccgt ccgagcgttg tgagcgacgt gcaaggcgac atcggagccg
     5401 tatccgccgc gcagaaggcc ttcgacagcg tggaacgaac gggactccat cgtatcgccg
     5461 caggcaaggt cgtccgcctc gccagccgcc gccactgagc gcagttttcc gggcttcggt
     5521 ttctgaagct aaaaaacggg ccgcgatgcg gcccgtttca ttatcgctgt gggttttatc
     5581 aacgtttccc gaccttgtcc ttttcgatca atgcatatgc ggagtgatta tggattgatt
     5641 cgaagtttta gttccggccg ctacgcttaa tcggaccaca tgtggtccga ttagcctgca
     5701 ctgaaatttc gagtccggag gctcagttct tcgtcgcagg cttagacttg tccttttcga
     5761 tcagcgcgta tgccgaatga ttgtgaatgg actcgaaatt ttcactctcc accacgtagg
     5821 catcgatgcg cttgtcctgg ttcagcaccg tagcgatatc gcgcaccatg tcctcgacaa
     5881 acttgggatt gtcgtaggcg cgctcggtga cgtatttttc gtccggacgc tttagcaggc
     5941 catagagttc gcaggacgcc tggtcttcgg cgatacggac caggtcctcg atccacacgt
     6001 gcgtgttggt gcgcgcgctg atggtcacgt gcgagcgctg attgtgcgca ccgtattcgg
     6061 agatcctctt cgagcacggg cacaggctgg tgaccggaac caccactttc atggtgaaaa
     6121 cgtactggtt cctgaaaacc tcgccaacga acgtcacctc gtagtccatc aggctcttga
     6181 ccccggacac gggcgccgcc ttgttgatga aatacggaaa agtcatttcg atatggccga
     6241 actcggcctc cagccgtttg accatgtcgc gcagcatcgc ctcgaaggac tccaccgaga
     6301 tttcacgttc gtgcgagttc aggatctcga cgaagcgcga catgtgtgtg cccttgaagt
     6361 tgtgcggaag ccccacgtac atattgaacg tggcgatggt gtgctggacg ccgtcgctac
     6421 ggtccgccac ccgcaccgga tggcggatgg ccttgattcc gaccttgtcg atggcgagcc
     6481 ggcgggtgtc cgccgaactc tgtacgtcgg ggatcgagaa gccttcgggg gaattcatga
     6541 ctgggtccct ggatttgagc acatgcggcc gattatagac gccgccaata gttgagtaaa
     6601 ctactcggct aattttatcg caattgacgc gtggatgccc tccgcatcca gtccgcattc
     6661 cttgagcaat cgcgccgggt cgccgtgatc gacaaaacga tccggcaacc cgagttgcaa
     6721 aactttcttc tgaattccct ttttttcaag agcttccagg actgcgctgc cggcaccacc
     6781 gaggatggtg ttctcttcca cggtgaccag ccattcgtgt gagatcgcca gttctcggat
     6841 cagttcttcg tccagtggtt tcacgaaacg catgttggca actgtcgcgt ccagtttttc
     6901 tgcggcttcc acgcaaggct tgaggagcgc gccgaaagac aggatcgcga cccgctggcc
     6961 gcggcggcgc acttcgccct tgcctatcgt cagcgccgtc aggtccttct ggactggcac
     7021 gcccggcccg ctgccgcgcg ggtagcgcac cgccgccggg ccatcgatct ggaacccggt
     7081 atgcagcatc tggcggcatt cgttctcgtc acttggggcc attaccacca tgttgggcag
     7141 acagcgcagg taggtaaggt cgaagctgcc ggcgtgcgtt ggcccatcgg cgccgaccag
     7201 cccggcgcga tcaattccga acaagaccgg caggttctgg atgcagatgt cgtggatcaa
     7261 ctgatcgtag gcgcgttgca gaaaggtcga atagattgcc accaccggct tcaaaccctc
     7321 gcaggccaat ccggcggcaa aagtgagcgc gtgctgctcg gcgataccga cgtcgaaata
     7381 ccggtccgga tactcctggg agaaacgcac caacccagaa ccttcgcgca tcgccggagt
     7441 tattccgatc aggcgcgaat ccagagccgc cgtgtcgcag agccagtcgc caaatatctg
     7501 ggtgtaggtc ggtttcccgc cggacttggg tgtgatgccc gcgtccgggt cgaacttggt
     7561 taccccgtgg tacaggatcg ggtccttttc cgccggctcg taccctttgc ctttgcgggt
     7621 gacgatgtgc aggaactgcg gtccctcgag ttttttcagg ttgctcaggg tcgacaacag
     7681 cacgtcgagg ttatggccgt ctatcggacc gatgtaattg aagccgaact cctcgaacaa
     7741 ggtgccgggc gtaaccatgc ccttcacgtg ttcttcggcg cgcttggcca gttccagcat
     7801 cggcggcacc actccgagca ctttctcgct ggtcttccat gccgcggcat agaaacgccc
     7861 ggacatcaat ttcgccaggt agttgttgag cgcgccgaca ttttcagaga tcgacatgtc
     7921 gttgtcgttc agtacgacca ggagattggc atccatcgct cccgcattgt tcagcgcctc
     7981 gaatgccatg ccggcggtca ttgcaccgtc gccaatgatc gccaccaccc gcctgtcctc
     8041 acccttcatg cgcgccgcca ccgccatgcc gagcgcggca ctgatcgagg tgctggaatg
     8101 cgcggtgccg aaagtgtcgt attcgctttc gatgcggcgc ggaaatccgg ctacgccgcc
     8161 gtactgtcgc agcgtcgaca tccgctcccg ccgcccggtg agaatcttat gggcgtaggt
     8221 ctggtggccg acatcccaga ccagacgatc atgaggcgta ttgaatacat agtgcagcgc
     8281 cacggtgagc tcgaccgtgc cgagattgga cgacaggtgg ccgccggtgt tcgccaccga
     8341 atgaatcagg tagtcacgca attccctggc gagcgccgcg agctgcttgc gttcgagtgc
     8401 gcgcacgtcc gacggattgc tgatcttctc gagaagttcg tacatgtccg tgaccgaatt
     8461 cagaaccgcc gcaacacgat aaaatcagcg agtgggcgaa gctgtccgcc attctgagga
     8521 agcgcgtcca gtgcgcgcaa tgcgtcgtcg cgcaattcgg cggcgaattt gcgcgcccct
     8581 tcgagaccaa gcgcgctcac gtaagtcggt ttgttgttct tcgcatcctt gcccgcggtc
     8641 ttgcccagcg tcgctgtgct ggactgggca tccagcacgt cgtcgaccac ctggaaggcc
     8701 agaccgatga acttgccaaa gcgatccagt tccttgaact gcgcttgtga catcgcgtcg
     8761 ccgcaacgcg cacccagcgc cacggaggcc cggatcagtg caccggtctt gtggacatgc
     8821 atgaattcca gctcggttac tgcgagcgtc ttgccggtgg cctcgaggtc gattgcctgt
     8881 ccgcccgcca tgccgcgtga gccacaggca cgcgcgagaa attcgatcat ctccacctgg
     8941 tgctgcgcgt tgtcggccat cgattgctcg gatatgactt gaaaggcaag gctgtgcaag
     9001 ctgtcgccga ccagcagagc ggtggcttcg tcgaattcca gatgcaccgt gggcttgcct
     9061 ctgcgcagca catcgtcgtc catgcaaggc agatcatcgt gcaccagcga atatgcatgg
     9121 atcatttcca ccgcggccgc cgcgcgatcc acccgctgcg ggtcggcacc ggagagctcg
     9181 ccggccccgt aagcaagcag cggcctgaca cgcttgcctc cacccaacac ggcatagcgc
     9241 atcgccgcgt gcagacgggc gggggcaagc tccgctgccg gcaacaaggc attcagggtc
     9301 ttttcgatgc ggtcctgacg gtccagcatc caaccctgaa aatcattcct tgccgtcgtc
     9361 accggaaaaa ttctgcagag tgccggcctc gaggaccttc acctgttgtt gggcttcctg
     9421 tagctgcgcc tggcaatact ggaggagctc cgcaccgcgt ttgtaggccg ccagggactg
     9481 ctcgagcgga agctgccccg cctccatggc agcgacgatg ctctcgatct cggcgagtgc
     9541 ctgctcgaac gattgcggct tctgcgattt gggtttggtc acggaggaca gatgatcggg
     9601 aggcgggaaa gttcgtaaaa atagcctatc cccggaaccc gggtcaacga atgaggtcgg
     9661 atggcgttga accgacgtgc catgaatgac ctaaagacgt tgcgccgggt agggttttaa
     9721 gggacaatgc ccaacggaat tccgcccaaa ctgtgcgtca agccgggggc ggttttttta
     9781 ttactgtttt gccccctggg ggacatccgg ttatgacaga tctggcaaat gtttccgctt
     9841 tgactcgaac gccggcacag ccgcccgtcg agtggtatct cgatccgcgc attgccagaa
     9901 tcgagcacaa agtgctgttc caggaggggc caggttacgt cggccacaaa ttgatggtcc
     9961 ccgaggttgg cgactatcgc accctcgaat ggatggggca cgccaaagcg ctggtgcaca
    10021 accagaacgg ggtggaactg ctgtccaacg tctgtcgcca tcgccagtcc atcatcctca
    10081 agggacatgg gcgcacacac aacattgtct gcccgttgca ccgttggacc tacgacacgt
    10141 cgggccgcct gctcggcgcc ccacactttc cgggcaaccc ctgcctggac ctggaaacga
    10201 cacctttggc ccagtggaac ggcctgctgt tcgccggtcc gcgcaatccg aatctggatt
    10261 tgtcgaaact gggcgtgctt gccgacctcg acttctcagg ctacgtgctc gaccgggtga
    10321 tgatcgaaga atacccggtc aactggaaga cgttcatcga agtttatctc gaggattatc
    10381 acatcgggcc atttcatccg gggctgggcc agtttgtcga tgccgagaat ctgaagtggg
    10441 aattcggcga gtggtacagc gtgcagaccg tggggatcaa tcaggcgctc gggaggccag
    10501 gcacggatgc atacaagaaa tggcacgagg cggtgcttcg ctacaacaat ggcgagatgc
    10561 ccaagcaggg cgcaatctgg ctcacttact atccgaacat catggtggag tggtatccca
    10621 acgtgctggc ggtcagctcc atcgtgccgc gcggacccga acactgcagc aacgtggtgg
    10681 agttctacta tcccgaagac atcgttctgt tcgaacgcga gtttgtcgaa gcggagcaac
    10741 gggcctacat cgagaccgcc agagaagacg aggaaatctg tctgcgcatg accgaaggcc
    10801 gccgcgcgct ctaccagcag ggccgcaacg aattcgggcc atatcagtcg ccgatggaag
    10861 acgggatgat ccatttccac gaattcctgc gcaatcatat cgagccgcac ctgtaagtcc
    10921 gcgcgggtac cgcgaacggg cgaacttgct gcacggccgc gggtggcgcc gagacgctcg
    10981 atactcaatc ctctcgcctc acaacccacg ccttccgcat gaagtacaca actctcattt
    11041 ccgtcgccga actggcaaat cacctcggca atgcggaact cgtgatcttc gactgccgtc
    11101 acgaattgac caatcccgaa tacggaccga atgcgtacgc cgagtcgcat atccccggcg
    11161 cccgtttcgc gcatgtggat agggatcttg cggggcgact cgccggcaaa aatggccgcc
    11221 atccgctgcc tgacccgaac gccttcgtcg cctggctcgg ccgcatgggt gttggcagcg
    11281 gcagtcaggt cgtcggctac gatcatgccg gcggcaccta tgccgcgcgc ctgtggtgga
    11341 tgttgcgctg ggtcggacat cggaaagtgg cggtcgtcga cggcggttgg caggcatggg
    11401 ccgccgccgg caatcctgtg accaaggaac tgccgacgcc gacggcagca aagttcagcg
    11461 gcaaacccga ccgctcctgg gtggacgctg cggccgtcac caaacgcctt aattcgaagg
    11521 aaatgatcct gatcgacgca cgcagcaacg accgtttcca tggccgcaac gagaccatcg
    11581 atccggtcgt cggccacatt ccgggcgcgc tcaaccgctt cttcaaggac aacctgaaca
    11641 cccagggttt cttcaagtcg ccgcaggaac tgcgcgccga cttcaattcg ctggtcggcg
    11701 atgcgtcacc ggaaaaagtc gtgcatcaat gcggctctgg cgtgtccgcc tgccacaatc
    11761 tgctggcgat ggagatcgcc ggcatcaagg gcacccggct ttatcccggt tcgtggagcg
    11821 agtggatcgc cgatccatcg cgccctgtgg ccagctagag gtcttcaccc ggcgacgtga
    11881 gcggactcgc cgacgtcgtt ctcctggtcc acgccctgtt cgtggtgttc gttgtcggcg
    11941 gcctggcgct catctggatc ggggccttgt gcggttggcg gatcgttcgt aatttcccgt
    12001 ttcgcgtact gcacctggct gcgatcctct ttgtttgcgc cgaagcgctc atcggtgtcg
    12061 cctgtccgct caccgcctgg gaggatcagt tgcgtggcgg tcctgcgcaa agcgatttcg
    12121 tcgcccgctg gctgcaccgc atcctttact acgattttcc cggatgggtt ttcctgaccc
    12181 tgtacctctt gttcgcattg gcggtcgcgg gatcgttcct tctaatccgt ccaagtcgcg
    12241 atcacgaccg gaataagaac gggcggcaaa gccgcccgtc cgggtaattc cacaaacgtg
    12301 tgtcagccga gcatgtcgga ccagatattc ctgccccagg cttccgcgta ctggccttcc
    12361 agttcgctcg cggccgcaga cacaccgctg cctcccggga cgatctccat ggttttcttc
    12421 tccggcacat aggcatgcac ggaagcaacg tggatgactt ccttgtcgct cacaaagctg
    12481 tagcaggtgt tggtgaccag tggctggcta ttgaccgctt ccccatgcag cagcgccacc
    12541 accgcggcag cacagacttt cgcgtgctgg ttggccatat gcccggattt gggcatctgc
    12601 ggtgcagcca tgatggcgtc acccagtacg tgcacgttct tcacggccga tgactcgtac
    12661 gtcagaaaat ccacaccgca ccacctgttg ttcgcggtga tcaacccggc atctgcggca
    12721 atcttgccgc agcgctgcgg cggaaccacg ttcaacacat cggccttgac gtcttcgaat
    12781 gcaagtttcg ccgtcatggt ggctgcatcc actcccgtga gttcgctgtt cggtcggtac
    12841 tcgatgatcc cggcgtagcg ctcgctccac gctttcgtga acagcccctt cttcgacacc
    12901 acatcctcgt tggcgtcgag gatgaggacc ttggatttcg gtttgctggt cttgaagtac
    12961 cacgccacct ggcaggcacg ttcgtacggt cccggcgggc agcggtacgg ggccttggga
    13021 atcgtcatcg cgtacacgcc gccgtccgcc attgcctcca gctgcttgcg cagcgccacc
    13081 gtctgcggcc cggccttcca ggcgtgcagc acccgcgcct gcgcctcggg cgaatccagg
    13141 cccggcacct cgttgtagag tatgtccaca ccgggcgcca cgatcaggcg atcgtatttg
    13201 agggtgccgc ctttgcccaa tcgcacttcc tgcgcgaccg gatcgatcgc ctttgcctcg
    13261 tcgcggatca gtttgactcc atacttggcc tgaagcttgc catagtcgcg ggtgaggtcc
    13321 ttcaactgga tgacgccagc aagcacgcgg ttcgaaagcg gacaggaaac gaactctgcg
    13381 tcccgctcga ccagagtgac ctcgatatcc ggcccccaca tgcgcacata tttggcggcg
    13441 gtcgcaccgg cgtagccacc tccaattacc actaccctgc ccacggtctt ggacgtcgtg
    13501 gcggtcgcgc aaccgatgct tccggataac gcagccgaag ccgacaacca cttgacgaaa
    13561 tcgcgacgtg tcaaagccat tttccctctc ctcggttgcc gggcttcact tcaacggttg
    13621 cgcagcgaag aactccgcga ttcgctcgat ctgctcatcg gagtagccct tggcgtgctg
    13681 atgcatgacg gttgccggcc ttgtacccgc cttgaactcc ttcatggcat tgacgatgta
    13741 cgccttgtcc agaccggcca gcaacggcat accgccctcg ctatggccgt tggtgccatg
    13801 acagcccgtg cacgttgccg cccaattgcg cccattgatg ttgttgtcca ccgcgagtgc
    13861 gggaatgctc gctccggcga gcagcagcgc cgcgtatgtg atgcctgccc ttatcattgc
    13921 tgttctccca ttcttgacgc gtgactgcaa cgccgtgtgc ggtttattcg catacacgtt
    13981 actcgcggcc gggaggcggc gcaacccggc cggttcgccc gggtgcagtc cggcgtcacc
    14041 gaaaaaaaag ccggacctcg tcggtccggc tcgggttgca ccattgcgtt cgttcaggcg
    14101 agactttctt ccttggcctc gtcgaaaagc agcctggtct tgccttcggc atccacgtcc
    14161 acggtaacct tgccgccgct cgccaggcgt ccaaacagaa gctcgtcggc cagcgcagta
    14221 cgtatcgtgt cctgaatcag ccgcgacatg ggccgcgcgc ccatcagcgg atcaaagccg
    14281 ctcttggcga gatacgcctt tagcgcatcg gtgaaggcca cttccacttt cttctcgtgc
    14341 aattgctcct cgagttgcat gagaaatttg tccaccacct tgatgatgat gtcgtgggac
    14401 agcgccgaga aggaaatggc ggcatccagc cgattgcgga attccggcgt gaacatgcgc
    14461 ttgatctccg ccatctcgtc tccggcaacc gtctgctggg tgaatcccat cgatgtcttc
    14521 gacagctctg aggcaccggc gttggtggtc atcacgatca cgatgttgcg aaagtctgcc
    14581 ttgcgcccgt tgttgtcggt gagcgtgcca tggtccatta cctgcagcag gatgttgaaa
    14641 atgtccgggt gcgccttctc gatttcgtcg aggagcagta ccgagtatgg atgcttggtg
    14701 attgcctcgg taagcagtcc gccctggtcg aaaccgacgt aaccgggcgg cgcgccgatc
    14761 aggcgcgaca ccgcatgcct ttccatgtac tcggacatgt cgaagcggat gagttccaca
    14821 cccatcgtgt aagcgagctg gcgcgctact tcagtcttgc ccaccccggt ggggccgctg
    14881 aacaggaagc aaccgatcgg cttctgcgga ttgcccaggc cgcttctcga catcttgatc
    14941 gaggcggcga gcgcgtcgat cgccttttcc tggccgaaca ccactgcctt caggtcgcgg
    15001 tcgagattct gcaacttgct gcgatcggtg gaagacacgc tctgcgccgg cacgcgcgca
    15061 attttggcga tgatctcctc gatctcgtgt ttgctgatga cgcgtttctg cttggacttc
    15121 ggcaggatgc gttgcgccgc gccggcctcg tcgatcacat cgatggcttt gtcgggcagg
    15181 tggcggtcat tgatgaagcg cgcggacaat tccgccgccg aggttagagc cgaggcggtg
    15241 tacttgaccc cgtgatgtgc ctcgaaacgc gtcttcaggc cgcgcaggat ctcgacggtt
    15301 tccgcaaccg aaggttcggt cacatcgatc ttttggaagc gtcgcgacag cgcatggtcc
    15361 ttttcgaata tcccgcggaa ttcggtgtag gttgtggcgc cgatgcactt cagggccccg
    15421 gaggacaacg ccggcttgag caggttggag gcgtccagcg tcccgccgga ggctgcaccg
    15481 gcgccgatga gcgtgtggat ctcgtcgatg aacaggatcg cgttggggtt gtcggtaagc
    15541 tgcttgagca ccgctttgag ccgttgctcg aagtcgccac ggtacttggt gccggcaagc
    15601 agcgcaccca tgtcgagcgc gtagacctgg cagcgcgaca gaatctcggg cacgctgccc
    15661 tcgatgatgc ggcgcgccaa tccttcggcg atcgcggtct ttcccacccc ggcctcgccc
    15721 accagaagcg gattgttctt gcgccggcgg cacagcgtct ggatcacgcg ctcgagttcg
    15781 cgttcccggc cgatcagcgg atcgatcttg ccggagatgg caagcgcgtt caggttcatg
    15841 gtgtagctct cgagcgcgcc ccccgaagac tgctcctgct cggaatccgc gtccgcctcc
    15901 ggttgctgct tggcgggcgc cgcctgcggc actttggtga tgccatggga gatgaagttc
    15961 accacgtcca ggcgcgtcac ccccttctgg tgcaggaagt acaccgcatg cgagtccttc
    16021 tcgccaaaaa tcgcgaccag cacattggcg ccggtgactt ctttcttgcc cgaggactgc
    16081 acatgcagaa tcgcgcgctg gatcacccgc tggaatccaa gcgtcggttg ggtgtcgact
    16141 tcttccccgc tcaccaccgg ggtgtgttcg ttgatgaagt cgttcaggag ctttctcagt
    16201 tcctcgacat tggcagcgca ggcgcgcagg acctctgcgg cggacgggtt gtccagcatg
    16261 gcgagcagca ggtgctccac ggtgatgaat tcgtggcgct tctgtcgtgc ttccataaac
    16321 gccatgtgaa gactgacttc taactcctgc gcaatcatgt ttcctccatc acgcactgaa
    16381 gcggatgctg atgctgcctc gaaaaagcta taacctgctc aaccttggtg gcggccacat
    16441 ccttaggata caccccgcac acgcccatgc cttccctgtg tactttgagc atgatctggg
    16501 tcgcctgttc tcgtgtcatc gaaaaaaacg tctggagaac ggagaccacg aagtccatgg
    16561 gtgtgtaatc gtcgttcaac agcagaactt tgaacagggg cgggggcttg accttgcttt
    16621 tctcggcctc cagaagcgtg ctgtcgcgag ttcccgatgc cataacctgc tgtctcgctt
    16681 ccactgcctt caccaagatc taattttgct gataaccgtg gaaattacaa gtgcagcatc
    16741 cggactcggc cagagaattg gcagattccc taaaaccgca ttgcaacaac ctcttgcgaa
    16801 ggttttggcg tacttgactt cccaaaataa atgacctaaa aagtggatag gtgtttggct
    16861 tcaaggatac tgcagtaccg gttttgccga taacgcggac ttgaaactca aacaatcggc
    16921 gggtttccga cccgcgtttt tcatcagggg aaggggagta tggcaactgg cactgtaaag
    16981 tggtttaacg acgcgaaggg ctatggtttc atcacgcccg acgacggcag cgaagatctg
    17041 tttgcccatt tttcggcaat tcagatgaac ggcttcaaga cgctcaaaga aggtcagaag
    17101 gtgagtttcg aagtcaccca agggccaaag gggaaacagg cctccaacat ccaggccgcc
    17161 taaaggctgc cgggcggcgc aaacgactcc gccggcaggt tcagaacatt cgaagcccgc
    17221 gacgccatcg cgggcttttt gtttttgcgg gttgctcaat ccttcatgcg ctcgatcatg
    17281 gcgttgccga acccggagca ggagacctgt gtcgccccgg tcatcagacg cgcaaagtca
    17341 tacgtgacga ctttgtcgcg gatagccgcc tccatggatc tgacgatgag atcggccgct
    17401 tccacccaac ccatgtgacg cagcatcatc tccgccgaga gaatcagcga acccggattg
    17461 acgtagtcct tgccggcata cttcggcgcc gttccatggg tcgcctcgaa catcgccacc
    17521 gagtcgctgg tattggcgcc gggcgcgatg ccgataccgc ccacctgggc cgccagggcg
    17581 tcggaaatgt agtcgccatt caggttgagc gtagcaatga cgtcgtattc gtccggacgc
    17641 agcagaattt gctgcaggaa agcgtcggcg atcgcatcct tgaccaccat tccgttcggc
    17701 agtttcatcc acggcccgcc gtcaagcagc gccgccccaa actcgcgctt ggcgagcgcg
    17761 taaccccagt cccgaaaagc gccttcggtg aatttcatga tgttgccctt gtgcaccagg
    17821 gtgaccgacc tgcgcttgtt cgcgatcgcg tactggagcg ccttgcgcac cagccgctcg
    17881 gtgccttcgc gcgagactgg cttaatgccg attccagaag tccggggaaa acggatcttc
    17941 ttcaccccca tttccttgat caggaaatcg atcaccttgc gggaatcttc cgattccgcg
    18001 gcccactcta tgcctgcgta aatgtcctcg gagttctccc gaaagatcac catgtcggtc
    18061 ttttcgggct gtttcagcgg gctgggaacg cccttgaagt agcgtacggg gcgaagacac
    18121 acgtacaggt cgagttgctg gcgcaacgca acgttaatcg aacggatgcc gccacccacc
    18181 ggcgtcgtaa gcggcccctt gatcgagacc acgaactcct tgctggcctg cagcgtctct
    18241 tccggcagcc agacgttttc gccatagacc gtgcacgatt tctcgccggc gtacatctcc
    18301 atccacacga ccttgcgctt gctttcatag gccacctgga ccgccgaatc cacgaccctg
    18361 cgcatcacgg gcgtaatgtc cacaccggta ccgtcacctt cgatgaaggg aatgatcggg
    18421 ttggcgggaa cgttcaacat gtagtccttg tttacggtga ttttctgccc tgtggaagga
    18481 accttgatgt gctggtacat gaaatcggcc tcgattagac gtgtagatgg atgtgattga
    18541 attgcaagca gtaatgcgaa gctgtcaaag gcgccaattg taaagcaagg tccgctcgcg
    18601 gcccaccgga acccgctctg aacacacgcc cgatccttta gctgtgattt ctccggcaac
    18661 gcggaaaacg aatcacctga ccagccttct acaacgctcc acgcatactc gggtcaggtg
    18721 tagtcctcgt ggcgacacgc atccaaccac ggcaataggc caacgaactc gaatcggcaa
    18781 agtccttgaa accaaatttc gcattgcaaa atcgcgcgcc gtgaaaattc ataagtttat
    18841 attttttaat gtgaaattcc agttctgctt tattgttgcg gcgcatgaaa atcctgatgt
    18901 actgtcgccg gcaccaccgt tgcgccaata aacgctggcg caacgcggat tcgaggccga
    18961 gctcaagcac gctgcctcca agccgtgtgc cagatccgat ctctggcttc ccgtggttga
    19021 gagtgccggc ccaccggtac tcggcaagca gggatctgtg tccacgatgt ttcgcgtgac
    19081 ccgcgacaca agtacggcat gacctgttgc gaggacgcag cccgattgcg tccaaaccga
    19141 gacacacacg tacttgccga aaggagaatg ctcatgaccc gcgatctgga aatcacaaga
    19201 atcaagaagg attggtctga tcatccgcgc tggaaaggcg tgcaacgtac ctactccgcc
    19261 gaggacgtct accggctacg cggcacgctg gccgtcgagt acacgctcgc ccgtcgagga
    19321 gccgaaaaat tgtggcagct tctcagggat gagccgttcg tcaatgcgct tggcgcgctt
    19381 accggcaatc aggccttgca gcaggtcaag gccgggctca aagcgattta tctctccgga
    19441 tggcaggtgg cggcggatgc caacctttcc ggcgagatgt atcccgatca gtcgctttat
    19501 cccgccaaca gcgttcccgc cgtggtccgc agaatcaata acaccttcat ccgcgccgac
    19561 cagatccatc acgccgaagg caatgacgac atcgactggt tcgcccccat cgttgccgat
    19621 gccgaagccg gtttcggcgg cgtgctgaac gcattcgagt tgatgaagtc gatggtcgaa
    19681 gccggcgcgg gtggcgtgca tttcgaagat cagctggcct cggcgaagaa gtgcggccac
    19741 atgggcggca aggtgctggt accgacccag gaggccgtgc aaaaactcgc cgccgcccgg
    19801 ctggcttcgg acatcatggg tgtgcccaca gttctattcg cacgcacgga cgccgaagct
    19861 gcaaatttgt tgacctctga tttcgacgag aacgacaaac cgttctgtac cggcgagcgc
    19921 accgccgaag gctactaccg ggtgatcaac ggcatccagc aggcaatttc gcgcgggctt
    19981 gcctacgcgc ctttcgccga cctgatctgg tgcgagaccg gcaaacccga cctcgcgttc
    20041 gccaggcagt ttgccgaggc gatccacaag cagtacccgg gcaagctgct cgcttacaac
    20101 tgctcgccgt cgttcaactg gaagaaaaac ctggacgatg catcgatcgc caggttccag
    20161 cgcgaactgg gtgcgatggg ctacaagttc cagttcatca cgctcgccgg cttccactcg
    20221 ctcaactaca gcatgttcga actggcgcgc ggctatgcca ccgagaacat gagtgcctac
    20281 gtgcggctgc agcaggccga atttgccgcc gaagcacacg gctatacggc gaccaagcat
    20341 cagcgcgaag tcggcacggg ttacttcgac gacgtgaccc aggcggtgca ggccggacgc
    20401 tcttcggtca cggcgttgac cggttcgacc gaagaagaac agttcgtata ggcgcttctc
    20461 tccttcccgc gcggccgcgg tatggcaagc tgtgcttgcc gccgcggcct ttcactttta
    20521 tcatttcgcg aatttatttt tcacaatgta aaaaactcga accaaccgga agccccaaat
    20581 gactcagccg aacatcccct ccggcatgaa agtgcttgga cacatcaccc ccgaatacgc
    20641 gcagattctc acaggcggcg cgctcgcctt cctcgccaag ctgcatcgga acttcaacac
    20701 ccgccgtctc gagctcctgg agcgccgcgc gctgcgccag aaggaactcg actccgggaa
    20761 gctgcccgat ttcctggcgg aaacaaagca cgttcgcgaa gctgactggc aggttgcgcc
    20821 ggtaccgcgg gatctccagg atcgacgggt cgagattacc gggccaactg accgcaagat
    20881 ggtgatcaac gcgctcaatt ccgccgccaa catgttcatg gccgacttcg aagattcttc
    20941 gacgccgacc tgggacaatc agttgcaggg ccagatcaac gtgcgcgacg cgctcactcg
    21001 cagcatttcc ttcaccagtc ccgaaggcaa acagtaccgg ctcgccgaca agaccgccac
    21061 tttgctggtg cggccgcgcg gctggcacct ctgcgagaaa catgtggtgg tcgacggcca
    21121 gccgatgtcg ggatcactgt tcgacttcgg cctgtacttc ttccacaacg ccaaggcacg
    21181 gatcaaggag agctcgggcg tgtatcttta cctgccgaaa atggaatcgc atctcgaggc
    21241 gagactgtgg aacgacgcct tcgagttcgc gcagaaggaa ctcggtgttc cacaaggcac
    21301 ggtaaaagcc acggtactga tcgaaaccat tctcgccacc tttgaaatgg aagaaatcct
    21361 gtacgagttg cgcgaacact cggcaggact gaactgcggc cgctgggact acattttcag
    21421 ctgcatcaag aaattccgga accggaagga ctttatcctc gccgaccggc atctggtgac
    21481 catggtgagc ccctttatgc gcgcttattc cctgctgctg atcaagacct gccacaagcg
    21541 cggtaccttc gcgatgggtg gcatggccgc gcaaattccg atcaagaacg atcccacggc
    21601 aaacgaggcg gcgctggcga aagtacgcgc cgacaaggag cgcgaggcca gtgacggcca
    21661 cgatggaacc tgggttgcgc accccgggct agtagcgatc gccaaggaag ccttcgacaa
    21721 ggtcatgaag accccgaacc agatccacaa gaagcgcgac gacgtcaacg tcagcgccaa
    21781 agacttgctc aatttccagc ccagggggcc agtcaccgag gccggcttgt gcaccaacat
    21841 caatatcggc atccagtacc tgggcgcctg gcttgccggc gccggctgtg tgccgatatt
    21901 caacctgatg gaagacgccg cgaccgcgga aatttcgcgg gcgcagatct ggcattggat
    21961 ccgcagtccc ttgggaatcc tggacgatgg ccgcaaggtc accagggagc tattccgtga
    22021 cctggtaccg caggaactcg aaaaagtgcg gcaattgctg ggcgacaagg gtttcgccac
    22081 cgggaaatat ccggagggtg ccaggatgtt cgaggagctg acgctcgccg aagactttgt
    22141 cgagttcctc actttgccgg cttatgacta cctcatcacg cacgagcacc gctgaatccc
    22201 gccggcccgg ccttagcggg tctaatccat gccgagggtt ctcctcttca acaagccctg
    22261 cggcgttctc tgccagttca gtccgagcgc gggcaagcgc acgctcaagg acttcatagc
    22321 ggtcgcggac gtctaccccg ccggccggct cgatgccgac agtgaaggtc tggtggtgct
    22381 gaccgacgat ggcaagctcc agcacgccat cagcgacccg aaaatgaagc tacccaagac
    22441 ttactgggta caggtcgatg gcgagcccga tgaagtcgcg ttgcggcgat tgcgctccgg
    22501 cgtcgacctc gacgacttcc tcacgcgacc ggcaggcgca cggcgcatcg accagccatc
    22561 cgggctatgg gagagaattc ccccgatccg cgtccgcaaa agcattccga ccagctggat
    22621 cgaactggaa ctgcatgaag gcaagaaccg gcaggtaagg cggatgaccg cggcggcagg
    22681 ctatccgacc ctgcgcctga tccgctcccg catcggaccc tggactttgg atggcatcct
    22741 gccgggccaa ttccgcttgg tcgaccacca gcaattgcgg atttgaagcg gattcagccg
    22801 cttgtaacca tttgtgtcaa ccgggcccat cggacggcgt tactgcacct gacgcccgaa
    22861 gggtgtctat acccccggta taaccagcct atggagagta ctgacatgaa gaagctattt
    22921 gctgccctga tcgccgccct gtttgccgca gtgactttct cggcggttgc tgccgataca
    22981 gcggtcaacc cggctgattc ggccaagccc gcgaaatccg ccaagaagca tcacaagaag
    23041 gcggcaaaaa aggttgaaga aactgctccg gccaagaagt aagcttcgaa gcggtttcgg
    23101 catcgaaagg caggccttag gcctgccttt tttcatttct tccggcgttt gacgctgtcg
    23161 caaaatatcc ttattctctg ccgcgaggac ggcaaggaaa aagaaccggc gaactaacgg
    23221 cggcgcgtcc ccgcggaaaa aggcagggcg gcgtccctgc cttttttcgt ttccctcggg
    23281 cttggagcag cactgaatcc ctgaatgagc gaatgggccg acatcctgaa gttgaccgtc
    23341 acgctgctgg ccatcgtcga tccgattgcc agcataccgg tgtttctgac caccactgat
    23401 ggcgagtcca acatgcagcg tgcacgcatt gcccggatcg tggcgatcac ggtgttctgc
    23461 gtcctcggcg cagccgcgct gatcggcacg gaaattctgc atttcttcgg gatcagcatc
    23521 ccctctttcc tgatcggcgg cgggatcgtg ctgatgatga tgtcgatctc gatgttgcag
    23581 gccaaggaaa gcaggatccg gcagacgccg gacgaagccg aggaggcggc ggaaaaacac
    23641 gcggtcgccg tggtaccgct cggcatcccg ctgctcgccg gcccgggggc aatcagcacc
    23701 atgatcatcg cgacccacca gtcgccgagt ttcatttatc acctcaagtt gctgatcccg
    23761 gcactgatca tcgccgtcgc ggtatgggca gcgctgatcg ctgccacccg gatttcaaag
    23821 cgcctcggca agaccggcat gaacatcatc acccgggtaa tgggcctgat catcgcggcg
    23881 atcgcggtcg agtacgttta ccgcggcctc atcgaactgt tcccgaaact gatctgagtg
    23941 actgcaaagt tgtggaagcc gaacgtgacc gtcgccgcga tcatcgagcg cgaaggaaga
    24001 ttcctgctgg tcgaggagga aacagacgac gggctgctgc tcaatcagcc ggcgggtcat
    24061 ctcgaggcca acgagtcgct ggtggaagct gcggcgcgcg aagcgctgga ggaaaccgct
    24121 tacgagttca cacccgagta cctggtcggc gtgtatcgct ggcggcaccc cgaccgcaac
    24181 gtcacttacc tgcgcttcgc ctttgccggc aggctgggta cgcaccaccc cgagcgcgcg
    24241 ctggatacgg gaatcgtccg ggcgttatgg atgactgcgg cggaggtccg tgccagccgc
    24301 agccgtcatc gcagcccgct ggtgattcgc tgcgtggaag actaccagcg cggtgcccgc
    24361 gccccgctcg atctgctggt gcatctcgac tgagcggaac cggcgaatgc aaaaaaggcg
    24421 cgtcgttgta ggcatgtccg gcggagtgga ttcgtcggtc gccgcctggt tgctcaagga
    24481 acagggcttt gacgtcgtcg gcctgttcat gaagaactgg gaagacgacg atgacgacga
    24541 gttctgcacc tcgcgccagg acctgatcga tgcggtctcg gtcgccgacc tgatcggcat
    24601 cgatatcgag gcagtgaatt tctccgctga gtacaaggac cgggttttca gcaacttcct
    24661 tgcggagtac caggccggcc gcacgcccaa tccggacgtg ctgtgcaacg ccgagatcaa
    24721 attcaaggcc ttcctcgatc acgcgctcca gcttggcgcc gaacagatcg ccaccgggca
    24781 ctacgcccag gtgcggcaag ttgacggcct gtttcaactg ctcaaggccg aggacggcac
    24841 caaggaccag agctatttcc tgtaccggct cgatcaggca cagctttcga agacgctgtt
    24901 tccactgggc gcgatgtaca agcgcgacgt gcgcgaactg gcgagaaaac tcgggctgcc
    24961 gaatcacgac aagaaggatt cgaccggcat ctgttttatc ggcgagaggc ccttccgcga
    25021 gtttctaaga cgctacctgc cgacccaacc cggggacatg gtgaccccag aaggcaagac
    25081 cgtcggcagg cacatcggcc ttatgtacta caccatcggc cagcgccagg gactgggaat
    25141 cggcgggcgc aaggacggcg aaggcgaacc ctggtacgtt gccgggaaag acatgacggc
    25201 aaacaggctt atcgtggtgc agggccacga ccatccggcg ctgttagcag atgccctgag
    25261 ggcgactgac ctgaactgga tcagcgacgc cgaaccgcat tgtcactggg tctacggtgc
    25321 gaaaacacgc taccggcaga aggacgcccc ttgcgaggtc gagcgcgtcg atcgggtgca
    25381 ctgtgagatc aagttcgctg aagcccagtg ggcaatcacg cccgggcagt ctgtcgtggt
    25441 ctacgaatcg aaggtgtgcc tcggcggagg aatcatcgaa agcccgaccg cagcaggcaa
    25501 gtaattgccc ctcacacgtc aagcccgacc ggaggcttca tcgaccttct agaacctggt
    25561 gcacagtccg acgcaggtat ggaatcacct cgccggcgaa ccaggggttg cgtttgagcc
    25621 acatctggtt gcgcgggctg gggtgcggca gcggaatgca atacggcaga tattcgcgcc
    25681 aggcacgcac tgtgtcggtc agcgtcgact tgcgccggtc gccgaggtag tacgcctgcg
    25741 cgtagctgcc tatgagcagc gccaaacgaa gtttcggcag atgtgccagc aattgcggat
    25801 gccaaagcgg cgcgcactcc ggccgcggcg gcaagtcccc gcctgcaccg gtccccgggt
    25861 aacagaatgc ggtcggaatg atggcgatgt ggcgttgatc gtagaaacgt tcccggtcga
    25921 gttgcagcca atcgcgcaac ctctctcccg atggatcgtt ccatggaatc ccggtttcgt
    25981 gcacgcggcg accgggcgcc tggccaacga tcagcagctg tgcggtaacc gaagcgcgca
    26041 gtaccggccg gggaccaaac ggcagatgcg cttcacatac acggcaggca tgcactttct
    26101 tcaacaacgc ttctaattcg cgtccgctgc gttgacctgg ggccgctctt ttggctgtct
    26161 tgcgggtcac cgaaaaactc cggccgcgac tctcatcgtg ttaaaattat tcatactgat
    26221 tagatacttg gatgactcac cccactcctc tcaccgccct gtccccgctt gacggccgct
    26281 atcgggacaa ggtggctggg cttacccatt acttcagcga atatggtctg atccgctacc
    26341 gcgtattcgt cgagatcgaa tggctgaagg cgttggcggc agccaagggg cttcccgaga
    26401 tcaagccgtt tgcatcggaa accatcacgc ggcttgacga actggcagcc aaattctcgg
    26461 tctccgacgg cgaggccgtc aaagccatag aagcccgcac caaccacgac gtcaaggcca
    26521 tcgaatactg gctgcgcgag aaactctccg gcaaccggga aatcgccggc atcacgcaat
    26581 tcattcactt tgcctgcact tccgaggacg tcaacaacct ctgtcacgcg ctcatgctaa
    26641 gggactcgcg ggcgcaggtg ttgctgccgg ccatcgatgc actggtggac aagctggtca
    26701 gcctcgcgca cgccaatgcc gatctgccaa tgctgtcgcg cacgcacggc cagcctgcca
    26761 cgccgagcac tctgggcaag gaactggcga acgtcgttca tcgtcttcgc cgccaacgca
    26821 atcgcatcgg cagcgttgca ctgaccgcca agatcaacgg cgccgtcggc aattacaatg
    26881 cgcacctcgc ggcttacccg gatttcgact gggagcaatt ctgccgctca ttcgtcgaga
    26941 tggtgggctt cgaattcaac ccctacacca cccagatcga gccgcacgac tgtatcgctg
    27001 aactgctcga tgcctacgcg gggatcaaca cggtgctgct cgacctcgac cgcgacatgt
    27061 ggggatacat ctccatcggt tatttcaagc agaggatcaa ggaaggcgag gtcggatcgt
    27121 ccacgatgcc gcacaaggta aacccgatcg atttcgagaa ctcggaaggc aatatcggtc
    27181 tggctaacgc cctgctcacc catctggctt ccaagctgcc ggtctcgcgc tggcagcgcg
    27241 acctcaccga ttccacggta ctgcgcaacc tgggcattgc gctcggttac agcctgctcg
    27301 cgtatgacgg ctgtctccgg ggcttgagca agctcgagcc ggacccgaaa cgcatcggcg
    27361 ccgatctgga agccaattgg gaagtgcttg ccgaaccgat ccagacggtc atgagacgct
    27421 acggagtcga agacgcctac gagcagctga aggcactcac tcgcggcaag gagggcatta
    27481 cccgcgagtc cctgcaccag ttcattcgca cgctcgccat cccccagccc gagaaagagc
    27541 gcttgctcag gctcacccct gccacataca ccgggcttgc cgcacagctg gcaaagcgca
    27601 tctgagaatc cacgcctacg atgcgcgccg agcgcgccag aactcgataa tccccggcat
    27661 gagcgaaagc acgatgatgc cgacaatgac gagggtgaga ttgtccttca ccaccggcag
    27721 attgccgaag aagtagcccg cgtaggtcag cgacgccacc cacagcatcg ccccggtcac
    27781 gttgaagagg agaaagtgcc ggtaacgcat ggcgccgatc ccggccacga agggcgcgta
    27841 ggtacggatg atcggcacga agcgggcaat gatgatcgtt ttgccgccgt gcctttcgaa
    27901 aaaagcgtgg gtgcgttcca gataggcgcg tctgaagaaa cgcgaatcct cgaagcggaa
    27961 cacccgcggg ccgagataac ggccgacggc atagttgacc gaatcgccga tgatcgccgc
    28021 catgatcagc agcatcacca acccatgcac gtgcagttct ccgcctgccg cgatcgtccc
    28081 ggcaacgaat aacagcgaat ctcccggcag gaagggcgtg acgaccagcc cggtctcgag
    28141 gaatacgatc aaaaacaata cggcatagat ccagttgccg taattctgcg ccagcgcgcg
    28201 caaatgctgg tcgaggtgaa cgaccagatc gaggaagcct gcgatcagat ccatggcgaa
    28261 tcggtcgctt tagacgacgg cttcctcttt cttcttctcg ggcctgatga aatcttcgcg
    28321 cgtcacgccc agccacatca ccagcgggct cgccaccagc acagacgagt agatgccgaa
    28381 caggatgccg atggtcagcg ccagagcaaa gtagtgcagc gtttcgccgc cgaataccag
    28441 tatcgaggtg accatcaact gggtactgcc gtgggtgatg atggttcggg acatggtacg
    28501 cgtaattgcg ttgttgatga tttccggcgt gcccgccttg cgcatcttgc ggaagttttc
    28561 gcggatgcgg tcaaaaacca ccaccgactc gttgaccgaa taacctagca cggcgagcac
    28621 ggccgccagc accggcagtg agaattccca ttggaagaac gcaaagaacc ccagaatgat
    28681 caccacgtcg tgcaggttcg cgacaatcgc ggagacgccg aatttccatt cgaagcgcaa
    28741 ccacagataa cagacgatgc cgatggacac gagcaacaac gccagcgcgc cgttctccac
    28801 cagttccttg ccgacctgcg atccaacgaa atccacccgc cgcagttcca ctgtccggtc
    28861 gtcctcgcgc agcgccttga tgacccgctc ggacagctgt gcgccggtta cgccctgctt
    28921 gctcggcagc cggatcaacg cggtctggga actgccgaaa ctctgcaccg tggcgtcgct
    28981 caagttaatg cggttcaagg cctcgcgaat tttcgagacg tcggccgcgt gaccgtagtt
    29041 cacctccatc accgtaccgc cggtaaattc gaccccgaga tgcaggcctt tggtcgccag
    29101 aaaaaacacc gccaggatga aggtgatcaa cgaaattacg ttgaacacca gcgcatggcg
    29161 catgaacggt atatctttgc ggatgcggaa gaattccatc tactcgacct ctgcggaaag
    29221 ctctgggcgg atgattctca ggcggccggc tgacgcctgt accaatcggt gttgccgatc
    29281 ggcagcttct ccagcctgcg acgggccccg aacgccaggt tgacgatgcc gcgggaaatc
    29341 accaccgcac tgaacatgga agtcataatg ccaagacagt gcacgacggc gaagccgcgc
    29401 accggcccgc tgccgaacat gaacaacgca atgccggcaa tcagcgtggt gatgttcgaa
    29461 tcgaggatcg tcgcccaggc gcgctcgtag ccggcagata tcgccgcctg cggcgtggtg
    29521 ccgttgcgca attcctccct gatccgctca ttgatcagaa cgttggagtc gatggccatg
    29581 cccagcgtca acgcgattgc cgcaatgccc ggcaacgtca gcgtagcctg cagcatggac
    29641 agcagcgcca ccagcagcat caagttggct gacagcgcga gtaccgagat cacgccgaat
    29701 acgttgtagt acacgatcat gaaaatggcg atcgcggtaa agccgatcag tgtcgaatcg
    29761 aagccgcgct tgatattgtc ggcacccagg ctcggaccga ccgtgcgttc ctcgacgatt
    29821 tccatcggcg ccgccagcgc gccggcgcgc agcaacagtg ccacgtcgcg cgcctcggtc
    29881 gtggtcatcc ggccgctgat ctgaacgcgt ccgcccggaa tctccgaacg gatcaccggc
    29941 gccgtcacga cttctccctt gcccttttcc accagcacca ttgccatacg cttgccaata
    30001 ctctcgcgcg tgatctgctg gaaaatcgtt gcgccgcggc cgtccagatt tatgtgcact
    30061 gccggctcgc cggtctggtt gtcgaatccg ggctgtgcgt ccgatatgcg atccccggtg
    30121 agaatcactt cgcgcttgag cagatacggc accccttcac ggtcgtaaag cagttcgtct
    30181 ccgaacggca cctgcccgcc gaccgccgcc tgcaacgacg cgctgtcgct gtgctcctca
    30241 tccaccatcc gtacttccag ggtcgcggta cggccgagaa tatccttggc cttggcggtg
    30301 tcctgcacac ccggcagttg cactaccacc cgatccagtc ccgcttgctg cacgatcggc
    30361 tccgccacgc cgagttcgtt tacacgattg cgcagggtcg tgatgttctg ctgcagcgcg
    30421 aagtcctgga ttcgcttccg cacttcgggc ttcagggaag ccgtaaccag aaactcgctg
    30481 ccggagtcgt caggcttgag cagcagatcc ggattctggc gctggatttc tgcggcagcc
    30541 ttgtcgcgcg cttcggcatc gcggaacctg atctgcaccg tctgcccttc ccgcgaaatg
    30601 ccgctgtaac gaatttcctt ggcgcgcagc gtggtccgga tatccgccgc gtaacggtcc
    30661 gccgccttgg tcagcgcgcc ggccatgtcg acctgcagaa ggaagtgcac cccgccgcgc
    30721 agatcgaggc ccaggtacat gggcaaagca tttatacggg tgagccagtg cggcgaactc
    30781 gacaacaggt tgagcgcgac gacgtagttc tcgcccagcg cactctgcag gacgtccttg
    30841 gccttgagtt gggtgtcggt gttgcccaga cgcaccttga tgctgtttgt ttcgaggaaa
    30901 acgccgtccg gaacaatgtt ggcctgcgcc agcgcctgtt ccacgcgcgt gcgcagcgct
    30961 tcatcgattt ttgcggtgga tttaccgggg gagatctgca ccgccggcac ttccccgaag
    31021 aagttgggca gggtgtagag aaagccgacg accagggcga cggcgatgat gacgtacttc
    31081 cagagcggat agcggttcat gtagggtccg tgcggcgcgc agcgtcaagc gtgaggattg
    31141 gcggactgtc gctcttcagc cttgaccggc ggtcttgacg gttcccttgg gcagcagggt
    31201 cgtgatcgag ggcttctgaa cctggatttc gatgttcgga gcaatttcca ccgacacgaa
    31261 gttatcgccc accctggtaa tttttccgag ggcgccgcca gcggtgacca cttcgtcccc
    31321 cttctgcaat ccggcaagca tggaccgatg ttccttggct cgcttggcct gcgggcggat
    31381 cagcaggaag tacagcagaa cgaacatcag gatgaggggc aggagtccga aaaggtccat
    31441 gcctcccgcg gccggcgcgg cctgcgcaaa cgcattactg atcaacacgt agttcctccg
    31501 gttcgggttc acaccgattt gtcggcgtga cactttttcc tgtcgtccgg tatcggttca
    31561 tgtgactcgt tgcgccactg caacgccggc gccgagcctg aaaagttgcg cattttagca
    31621 tggctgtgcc tgtccaagac gcaagccgtc gacatgttcc gaaagccttc ctgcgctgat
    31681 ggcctcgcgc aatccccgca tcaaatcctg gtagaagaaa agattgtgca gcgtgttcag
    31741 atgcgcaccg agaatttcgt tgattcgctg caggtgatgt aggtaggcgc gcgagaaatt
    31801 ccggcaggtg tagcatgcgc acgccggatc cagtgggccg gtgtcatcgc ggtagcgggc
    31861 attgcgcagc cggatgatgc cgttgcgggt gaatatccag ccattccggg cgttgcgcgt
    31921 aggcatgacg caatcgaaca tgtcgattcc gctcgcaacg gcatcgacga tgtccgcagg
    31981 cgtgccgacg cccatcagat agcgcggtgc cccggcaggc agcctgggcg cggtgtggcg
    32041 caggatgcgc tgcatgtcct ctttcgattc acccaccgac agcccgccga tggcgtagcc
    32101 gtcgaaggcg atgtccgtca gggcctcgag cgacgtgtcg cgcaggtcct cgtacatgcc
    32161 cccctgaaca ataccgaaaa gggcgttgcc gtttccctca tgggcccgcc tgctgcgttc
    32221 cgcccagcgc aaactcaact gcatcgactc tgccgcctgg ctgcgatcgg ccggatacgg
    32281 cgtgcactcg tcgaagacca tggcgatatc ggactcgagt acgcgctgga tgcgcatcga
    32341 ttcctcgggc gacaggaaac acgcgtcgcc gttgaccggc gagcggaatc tgacgccctc
    32401 ctccgtaatc ttgcgcattt cgccgaggct gaacacctgg aagccgcccg aatcggtgag
    32461 aatcggcccg tcccagccca tgaaacggcg cagcccgccg tgcgccccga tcacgtccag
    32521 accgggacgc aaccagaggt gaaacgtgtt gccgagaacg acctgcgccc cgacagtgcg
    32581 caggtcatcc ggactcatgg ccttgaccga gccgtaagtg ccgaccggca tgaatgcggg
    32641 ggtttccacc gtgccgtgcg cgagtgtgag ccggccccgg cgcgccggtc cgtccccggc
    32701 aatgatttcg aatcgcattt tcacgatctc gattgcgcgg ctttgtcgat caacattgcg
    32761 tcgccgtagc tgaagaatcg gtagcggcag tcgaccgcat ggcgataggc gcgccgtata
    32821 aattcggtcc cggcgaaagc acagaccagc atgagcaaag tggaccgcgg caggtgaaag
    32881 ttggtgagca ggcggtccac gaccttgaag ccgtagcccg gacggatgaa aagacgagtc
    32941 tccccgccgc cggaaatcat cttgcccgac tgtgccgccg actcgagcgc acgcaggctg
    33001 gtggttccga ccgcggtaat tcgcccgccg ccggcacgcg tttctgccac cgcctgcgcg
    33061 gtttcagcag agatctcgta gagttcgcca tgcatgacat gctgttcgac attttccacc
    33121 cgaaccggct ggaaggtgcc ggcaccgaca tgcagcgtga cataggcaaa ccgcacgtcg
    33181 atttctcgaa ggcgccgcaa cagttgttca tcgaaatgca ggccggcggt gggcgccgcc
    33241 acggctcccg gtacgcgcgc gtaaacggtc tgatagcgcg cctcatcgct gctgtcggcg
    33301 cggcgctcga tgtaaggcgg gagcggcact tcgccgagac gctcgagtac cccggctacg
    33361 tcgtcaccga actccagcag gaacaactct ccctgacgct cgatcatcgt ggcgtgcgca
    33421 agatcaccga atcggatccg ttgtccgggc ctggcagtgt ggctcgtcct caacatcgcc
    33481 aatgcccggc gcgggctcac gatgcgttct ataagggctt ctactttgcc ccccgactcc
    33541 ttgacgccct gtagcctcgc cttgatcact ttggtgtcgt tgaaaatcaa caagtcaccg
    33601 ctgtgcagga gcgacggcag atcgacaaaa aaatgatcgg cgagatttcc agtcgcgccg
    33661 tcaaggtgca acagccggct ggcactgcgt tgcgcggcgg ggacctgcgc gatcaagtcc
    33721 tgcggaagct cgtaatcgaa ttcttcgatt ctcatcgcgc gcattatatc ggccgcagac
    33781 cactcccccg gttgccccgc gacgccgaat tccgggataa ttgcgctccc gtgccggatg
    33841 gtggaatcag gacttgcacg gagattcacc aaataaatgc cgggatggcg gaactggtag
    33901 acgcacggga ctcaaaatcc cgcggtggcg acaccatggg ggttcgattc cccctcccgg
    33961 caccaggaac tttccaacgt cagcccttcg tcacggttcg cagccgccac tccggatcag
    34021 atcggctccg acgcgagaaa aatgaaacta ctcatcaaga agacagataa cgacggccgc
    34081 cttgccctca ttaccgactt tctgacgacc tcctggatca tcgaggtcgc ggacagcagc
    34141 aaccgcgagg aattggcaca caagattctg gatgccgacg cgatggtctc gatgaactgg
    34201 ccgacggaca tgccgccggc gcccaggctg cagctgcttc agcttcccgg ggccggcacc
    34261 gacgacattg ccttcgattc cgtgccggcg aaggtggcgg tatgtaacgt ctatgagcac
    34321 gaaatcggaa tagccgaata cattctctcg gcgatgctcc agtgggtaat cgggattcca
    34381 cggatggatg cggccctgcg ccgtggccaa tggtacggct cgcacctctc gggaccgcgg
    34441 cacgatgaac tgtatggcaa gaccgtcggc atcattggct atggccgtat cggtcgcgaa
    34501 gttgcgcgcc gggcgcgcgc cttcagcatg agggtgctgg cctgcagccg cactccccgc
    34561 agctccgacg ggttcgtgga gcgcgtggac ggaatggatc gcctgcctgc agtgttgtct
    34621 gcgtccgatt tcgttgttct ggcattaccg ctggagccgt cgaccgccgg ggtgatcggt
    34681 acgcagcaac tggcaaggat gaaaccgggc acggtgatca tcaacgtggc acgcggcgct
    34741 ctgatcgacg aacgcgcatt gttcgaggcg tgcagggaaa agcgcatcgg cggcgcggtg
    34801 atcgacacct ggttcaaata ccccgccgaa gggcatgagg tcgcggagcc ttccaacctg
    34861 cccttccgcg agctggagaa tgtcatcatg acaccgcatg cgtcaggatg gacggaggga
    34921 ttgcggccac gacgctgtag gatgattgcg ggaaatctgg atcggctggc ccgcggcgaa
    34981 cctctgatca acgtcgtaaa acaacctgcc ggcagccgat gagcggcggg tcaccggttt
    35041 cattcgagag ggaaaaaaag catggacaac tgggccgccg aacaggccgc cagaatcatc
    35101 tggagcacat ggcaggccaa tgatcgtgtc gatggcctcc ccgccgattg ccggccctcg
    35161 agccggcaag aaggttacaa agtgcagtcg gaagtggccc gtctgtccgg ccaggcgttg
    35221 ttcggatgga agattgccgc gaccagtcag gctggccaga agcatatagg cgtggacgga
    35281 ccgcttgccg gccggcttct cgagagccgc acgttccaga ctggcgcgag cgtaccgatg
    35341 gtcaacaacc tgatgaaggt agcggaggcc gagttcgcgt tccgaatggc tctcgctctg
    35401 ccgccgcgcg cgtccgcata ctcgatgcag gaagtactgg acgcggtgga atccctgcat
    35461 ccggccatcg aaattccgga ttcccgttat cgggaattcg cgaaagtcgg ggctgcgcaa
    35521 ctcatcgccg acaatgcctg cgctggctat ttcgtcctgg ggccggcgac tgccgccgga
    35581 tggcgtccgc atgacctggc cgaacacctc gcttcggcct gggtaaatgg caagctggca
    35641 cgcgagggca aaggcgccaa tgtgcttggc gatccacgtc tggctctcac ctggatcgtc
    35701 aatgaactct cggttgtcgg cgaaacgcta cgcgccggac atgtggtaac gacgggcact
    35761 tgcctggtgc cggtggagat cgcgcctggc gatcacgtgg tcgcggattt tggtctgttc
    35821 ggacgcgttg aagcgcgcct cgcaccctag cagcagagac gaactttc
//
LOCUS       DASQOP010000302         8120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1165457, whole genome shotgun sequence.
ACCESSION   DASQOP010000302 DASQOP010000000
VERSION     DASQOP010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8120
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165457"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..937
                     /gene="rsgA"
                     /locus_tag="VE030_12110"
     CDS             41..937
                     /gene="rsgA"
                     /locus_tag="VE030_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HZF24197.1"
                     /translation="MSERQPLLHGLIVGNFGRQYLVELTGSEIVTCVPRGKRSSLACG
                     DRVEILRTAPGQGVVEAVDPRSTLLYRSDQFRQKLVAANVTQIVIVLAVVPSFYEELL
                     NRCLVAAEHQRLSCLIVLNKFDLTNEDSTAMDRLTLYTDLGYRVTPLSAKRDVSPLRP
                     ALEGHTSVLVGQSGMGKSTIINALIPGTRAATSDVSAALDSGRHTTTHARLYHLDARS
                     HLIDSPGMQEFGLHHLTEQDVAGAFREFRPLNGRCRFRNCRHLVEPGCAIAQAVEEGS
                     IDKRRLDAYRKLAKEIESGKRF"
     gene            complement(951..1877)
                     /locus_tag="VE030_12115"
     CDS             complement(951..1877)
                     /locus_tag="VE030_12115"
                     /inference="COORDINATES: protein motif:HMM:NF005792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family protein"
                     /protein_id="HZF24198.1"
                     /translation="MSFFSLFAALLIEQVWPLNPRCTVYRWFTRYVNSLGGRFNAGEL
                     RQGVIGWCLAVLPWVIASIAVYHLLCEIHVVFGWAWNVAVLYLTMGFRQFSHAFTEMV
                     AVLRGGDLDKARQLLGEWRGESATEYNGTEIAKVAIEEGLIGAHRHVFGAIFWFLVLP
                     GPSGAVLYRLAGLLEQKWGGRSDQDYGEFGRFARKVFYYIDWIPVRLTAMSFAVAGDF
                     EDAVHCWRTQATAWMHPEQGVVLASGAGALGVRLGETLHHHGTVSFRPELGLGDDADV
                     NTMASAVGMIWRALVMWMLIIALIAIARWVGG"
     gene            complement(1973..2398)
                     /locus_tag="VE030_12120"
     CDS             complement(1973..2398)
                     /locus_tag="VE030_12120"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007747477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZF24199.1"
                     /translation="MKRKASAVWKGGLKDGKGTISTESGVLSDTQYGFKSRFENGPGT
                     NPEELIAAAHAGCFSMALSSQLGEAKLVAESIRTTASVSLDMIGGKFSITAVHLDLVA
                     KIPGASKEAFETAANNAKAGCPVSRVLSAKITLDARLES"
     gene            complement(2593..2964)
                     /locus_tag="VE030_12125"
     CDS             complement(2593..2964)
                     /locus_tag="VE030_12125"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HZF24200.1"
                     /translation="MRTTVCHCTFCQRRTGSAFAVIVYFGEKDVEFTAGAASSFEHRS
                     DESNRWLRIEFCPRCGTTVAHTAEVFPGVRAIAGGTFDNRAWFTIERHAWTRSACPWV
                     VVPEGVQVFSKGSVASIQKAP"
     gene            complement(3286..5586)
                     /locus_tag="VE030_12130"
     CDS             complement(3286..5586)
                     /locus_tag="VE030_12130"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZF24201.1"
                     /translation="MAKLDEYEDLLKALGPHARQLVQSTWLEATRSFTPEGLTRYLEG
                     AREIATAGLGWSVALAYLRETPAVAREIGEEAAFKTIDAALAVYARTDARTAEQVFAT
                     AIIAARRTHDGALYVSWLDFLRELGELAPAGVTAVLQRLDDLLEQLSFEGLRRWALLG
                     VQSHMRDPTAQARYFQLDSEEGRALLRAEGDQTVFADVERRLSLYLRALWGRSMKLRP
                     LIHTKGASSGRRSTMDGYTIRLPQAYRGFSGQSGLALYRAAVAHASAHIVFTTQRFPL
                     GSLKPLQVVLVSLVEDARVEQLAIRQFPGLRKLWQTFHVAQPGGAVTSASLMARLARA
                     LIDEDYRDDNPWVAKGQRQFFEQRAYWEDQATSRALGGLLGNDMGQMRVQFNHKTYVV
                     EPAYRDDNQGVWDFGDAGEQSAEDDDVVMQGARLTDGEKGPEREEDERPQPQAQEEEK
                     VRLSAPSRFSEALKEEPLSPPMRYDEWDYLIGRERPAWCTLLEKSPPEGNPHQIEEIL
                     ERNHDLVNRVKYLVKAVQVQRPVRLKKRLEGDRLDLDACISATMDLRTRIPPDPRVHA
                     ILGRQQRDLSVLVLLDLSQSTNDVVANAGTTVLNLAREATVLLADAMARIGDSFAIHG
                     FSSNGRHDVGYYRFKDFDRPYGELSKGRLAGMTGQLSTRMGTALRHAGTFLQDRRAHK
                     KLILLVTDGEPSDIDVHDARYLLFDAKKAVEENNRHGIFTYCMSLDPKADRYVSRIFG
                     QRNYIVVDHIRRLPEKLPMLYMRVTN"
     gene            complement(5827..6663)
                     /locus_tag="VE030_12135"
     CDS             complement(5827..6663)
                     /locus_tag="VE030_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HZF24202.1"
                     /translation="MNPNDSRNLSPAEGIVKQYRLDAEPYYRAVADEVQMFEAAYAVR
                     MPMMLKGPTGCGKTRFVEYMAWKLNKPLITLACNEDMTASDLVGRYLLDADGTRWQDG
                     PLTLAVRYGAICYLDEVVEARQDTTVVIHPLTDARRVLPLEKKNELVSAHPDFQLVIS
                     YNPGYQSILKDLKQSTKQRFGALDFGFPARDIEIEIVSHEAGIGPDLAAKLVAVAERA
                     RNLKGHGLEEGISTRMLIYAGSLIAQGAEPVAACHVTLIKPITDDPDIRDALGATIRA
                     CF"
     gene            complement(6667..7536)
                     /locus_tag="VE030_12140"
     CDS             complement(6667..7536)
                     /locus_tag="VE030_12140"
                     /inference="COORDINATES: protein motif:HMM:NF013899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HZF24203.1"
                     /translation="MKPFDLAGTALAAERPEEIHADIEFARDCGGATYVRRQRVGYPF
                     HLGRALRSPGDPAGMPTLYLQSCSGGIFEGDDLRLRIEAGEGAAAHVTSGAFTIVHGM
                     EEESAMQRLEIQAREGSFLEYLPDPAILFPRARLDSRVRVRVHPESSVILCDSVLLHD
                     PKGGDGVFDWLRSETRFEDPSGKLLVCDRFRVDGSQLARRLAGIAGASTAQGTLFIVT
                     GAKPATEIVAAMREALVLPGVYAGAALLPNRSGAWARILANDAAALKTAMFNAWSAAR
                     NLLTGAMPVQRRK"
     gene            complement(7533..>8120)
                     /gene="ureG"
                     /locus_tag="VE030_12145"
     CDS             complement(7533..>8120)
                     /gene="ureG"
                     /locus_tag="VE030_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HZF24204.1"
                     /translation="IGGPVGSGKTRLLEQLIPRFISRGTNLAVITNDLATKEDAERVR
                     RSGLIDPARVLAVETGACPHTAIREDPTMNIQAADELDAKYEKLDLILIESGGDNLAS
                     TFSLDLVDYWIFVIDVAGGDDIPRKRGLGVLQCDLLVINKTDLAPYVNVDLERMIREA
                     NEVRGGRPVQTTNCGTGEGVDQVVDAIAAQVLFDR"
BASE COUNT         1455 a         2646 c         2550 g         1469 t
ORIGIN      
        1 ctccgcgtcc tctgcggttg gcgttgttct tgaataaaag ttgagtgagc gccagccact
       61 tctgcacggc ctgatcgtcg ggaatttcgg ccggcagtat cttgtcgagc tgaccggttc
      121 cgagatcgtc acctgcgttc cacgaggcaa acgcagttcg ctcgcctgcg gcgaccgagt
      181 ggagattttg cgtaccgcgc ctggccaggg tgtggtcgag gcagtggatc cacggtccac
      241 gctgctgtac cgctccgacc agttccgcca gaaactggtc gccgcgaacg tcacccagat
      301 cgtcatcgtc ctggcggtcg tgccgagctt ctacgaggag ttgctgaatc gctgcctggt
      361 agccgccgag caccagcggc tctcctgcct gatcgtcctg aacaaattcg acctgacgaa
      421 tgaggactcc accgcaatgg accggctcac gctttacacc gatctcggct atcgcgtcac
      481 acccctgtcg gccaaacgcg atgtgtcgcc gcttcgcccc gcgctcgagg ggcacaccag
      541 cgtgctggtg gggcagtcgg gcatgggcaa gtccacgatc atcaacgcgt tgattcccgg
      601 aacccgcgcg gcgacctcgg acgtatccgc tgcgctggac tccggacggc ataccaccac
      661 gcacgcgcgc ctctatcacc tcgacgcgag gtcgcacctc atcgattccc cgggcatgca
      721 ggagttcggg ctccatcacc tgacggagca ggacgttgcc ggggcgttcc gcgaattccg
      781 gccgctcaac ggccgctgcc gcttccgcaa ctgccggcat ctggtggaac cgggctgtgc
      841 catcgcgcaa gcggtggagg aaggaagcat cgacaagcgg cggctggacg cctaccgcaa
      901 gctggcgaaa gagatcgagt cgggcaaaag attctgaggc ggcgaccggc ctagccgccg
      961 acccagcggg cgatggcgat cagcgcaatg atcagcatcc acattaccag cgcccgccag
     1021 atcattccca cggcactggc catggtgttc acgtcggcgt cgtcgccgag accgagctcg
     1081 ggacggaagc tcacggtacc gtggtggtgc agagtttcgc cgagccgcac gccgagcgcg
     1141 cccgcgccgc ttgcaagcac cacgccctgt tccggatgca tccatgcggt ggcctgcgta
     1201 cgccagcagt gcaccgcgtc ctcgaagtcc ccggcgactg caaagctcat cgccgtcagc
     1261 cgtaccggga tccagtcgat gtagtagaac acttttcgcg caaaccgccc gaactcgccg
     1321 tagtcctggt cggagcggcc tccccatttc tgttcgagaa ggccggccag ccggtagagc
     1381 acggcgccac tcggcccagg caggacgagg aaccagaaga tcgcgccgaa cacgtgccga
     1441 tgcgccccga tcaggccttc ctcgatcgcg accttggcga tctccgtgcc gttatattcg
     1501 gttgccgatt ctccgcgcca ttcgccgagg agctggcgcg ccttgtccag atcgccgccc
     1561 cgcaacaccg cgaccatctc ggtgaacgcg tggctgaact ggcggaagcc catggtcaga
     1621 tagagcaccg cgacgttcca cgcccagccg aagacaacat gaatttcgca cagcaaatgg
     1681 tagacggcga tgctggcaat cacccacggc agcactgcca ggcaccagcc gatcacgccc
     1741 tggcggagct cgccggcgtt gaaacggcca cccagcgagt tcacatagcg cgtgaaccag
     1801 cggtacaccg tgcagcgcgg gttgagcggc cagacctgct cgatcagcag cgcggcaaag
     1861 agggaaaaga acgacatggt gaaatattaa catcccccct ctccccgccc tctcccgcca
     1921 ggggagagag ggatcgccgt tttcaaccgg aatctggtga cgggccgcag ggtcaggact
     1981 ccagtcgcgc atccagcgtg atcttcgcgc tcaggactct ggacacggga cagcccgctt
     2041 tcgcgttgtt ggccgcggtc tcgaacgcct ccttgcttgc gcccggtatt ttcgcgacca
     2101 gatccagatg caccgcggtg atgctgaatt tgccgcctat catgtccagc gacactgagg
     2161 ccgtggttct gatgctctct gcaaccagtt tggcttcgcc caattgcgac gacagcgcca
     2221 tggagaagca gcccgcatgg gctgccgcga taagttcttc cggattcgtg cccggtccgt
     2281 tttcgaagcg cgatttgaat ccgtattgcg tatccgaaag aacgccgctt tcggtcgaga
     2341 tcgtaccctt gccgtccttc agaccaccct tccagactgc cgacgctttg cgcttcatgg
     2401 tgtccccctg ttggacaaag taatgcctcg catgcggtaa agcatacgtg cagccataac
     2461 gatccggatc agcgcgccgt tgaccctcgc gaacaggctg gtcacagcga ttagtgccgg
     2521 cttcgcaccg gtggtgcgct cgaatatcgg cccgagcagc accgcacccg ccgagagaac
     2581 gtattcagga tttcacggcg ccttctgaat cgaggccacc gaacccttcg agaacacctg
     2641 gacgccctcc ggtacaacca cccacgggca ggccgagcgt gtccaggcgt ggcgctctat
     2701 cgtgaaccag gcgcgattgt cgaacgtccc gccggcgatg gcacgaactc cgggaaaaac
     2761 ctcagcagta tgcgcaaccg tagtgccgca acggggacag aactcgatgc gcagccatcg
     2821 gttgctctcg tccgagcgat gctcgaagga actggcggct ccggcggtaa attcgacgtc
     2881 tttctccccg aagtaaacga tcacggcgaa ggcgcttccc gtgcggcgct ggcagaacgt
     2941 gcagtggcaa acagtggtgc gcacaggctc tcccgtggtc cggtaccgca ccgctccgca
     3001 aagacatcct ccctcatgga tttcaggcat ttacaagccc tcccccgaag cctgacgcgc
     3061 ggactcgtta gcgacggagt cgcccgtttg cgggtagcgc gtcattgctc cgctaccgcc
     3121 catttatgaa ccttgcgcgg tggtacagcg gagcaaccct ctcccgccag gggagaggga
     3181 atgtaaggtc aaaagcaaaa ggcgaataat tagtcgtcca tctaactgtg ttggacttag
     3241 ggctccctct cccccggcgg gagagggcgg ggagaggggg cggtcttagt tcgtgactct
     3301 catatacagc atcggcaact tctccggcag gcgccggatg tgatccacca ctatgtagtt
     3361 gcgctggccg aagatgcgcg agacgtagcg gtcggccttc ggatcgaggc tcatgcagta
     3421 ggtgaagatg ccgtggcggt tgttctcctc caccgccttt ttggcgtcga acagcaggta
     3481 tcgcgcgtcg tgcacatcga tgtcggatgg ttcgccgtcg gtgaccagca ggatcagttt
     3541 cttgtgcgcg cgacggtcct gcaggaaagt gccggcgtgg cgcagtgccg tgcccatgcg
     3601 cgtcgagagc tggccggtca tgcctgccag acgtcccttc gacaactcgc cgtatggccg
     3661 gtcgaaatcc ttgaagcggt agtagccgac gtcgtggcgg ccgttcgagg aaaaaccgtg
     3721 tatcgcgaag ctgtcgccga tgcgcgccat cgcgtcggcc agcagcacgg tggcttcgcg
     3781 cgccagattc aacacggtgg tcccggcatt cgcgacgacg tcattggtgg attgcgaaag
     3841 gtcaagcagc accagcaccg acagatcgcg ctgctgcctg ccgaggattg catgcacacg
     3901 cggatcgggc ggaatcctgg tgcgcagatc catggtggcg ctgatgcagg cgtcgagatc
     3961 gaggcggtcg ccttccagcc gctttttcaa ccggaccggc cgctgcacct gcaccgcttt
     4021 caccagatat ttcacccggt tcaccaggtc gtggttgcgc tcgaggattt cctcgatctg
     4081 gtgcggattg ccctcgggcg gcgatttctc cagcagcgtg caccaggccg ggcgttcccg
     4141 gccgatcagg taatcccatt cgtcgtagcg catcggcggc gacagcggct cttccttcag
     4201 cgcctcgctg aaccggctcg gggcggaaag cctgactttc tcctcttcct gcgcctgcgg
     4261 ctggggtcgc tcgtcttctt cccgttccgg ccccttctcg ccgtccgtca ggcgcgcgcc
     4321 ctgcatcacc acatcgtcgt cttccgccga ctgttcgccg gcatcgccga aatcccagac
     4381 gccctggttg tcgtcacggt aggccggctc gaccacgtag gtcttgtggt tgaactgcac
     4441 acgcatctgg cccatgtcgt tgccgagcag cccaccgagc gcgcggctgg tcgcctggtc
     4501 ttcccaatag gcgcgctgct cgaagaactg gcgctggccc ttcgccaccc agggattgtc
     4561 gtcgcgatag tcctcgtcga tcagggcgcg cgcaaggcgg gccatcagcg aagccgacgt
     4621 taccgccccg cccggttgcg ccacgtggaa ggtctgccac agcttgcgca gcccggggaa
     4681 ctgcctgatc gccagttgtt cgacccgcgc atcctcgacc agcgagacca gcacgacctg
     4741 caagggcttg aggcttccga gggggaaacg ctgcgtggtg aagacgatgt gagccgaggc
     4801 gtgcgcgacc gctgcgcgat agagagccag cccgctctgt ccggaaaagc cgcgataggc
     4861 ctgcggcagg cggatggtgt aaccgtccat ggtggagcgc cgcccggaac tcgcaccctt
     4921 cgtgtgaatc agcgggcgca gcttcatgct gcggccccat agcgcgcgca gatagagcga
     4981 aagccgccgc tccacgtcgg cgaacaccgt ctggtcgcct tccgcccgca gcagcgcgcg
     5041 gccttcctcg ctgtcgagct ggaagtagcg cgcctgcgca gtcgggtcgc gcatgtgcga
     5101 ttgcacgccc agcagcgccc agcggcgcag gccctcgaag gaaagctgtt cgagcaggtc
     5161 gtcgagccgc tgcagcacgg cggtgacgcc ggcgggcgcg agttcaccca gttcgcggag
     5221 gaagtcgagc caggagacgt agagagcgcc gtcgtgcgtg cgccgggcgg cgatgatggc
     5281 ggtcgcgaac acctgctctg ccgtgcgcgc gtcggtgcgc gcataaacgg caagcgcggc
     5341 gtcgatggtc ttgaaggcgg cttcctcgcc gatttcgcgc gcgactgccg gcgtctcacg
     5401 cagataggcc agcgccacgc tccagcccag gcccgccgtg gcaatctcgc gggcgccttc
     5461 gagatagcgc gtcaggccct ctggcgtgaa cgatcgcgtt gcctccagcc aggtggattg
     5521 caccagctgg cgcgcgtgcg gtccgagcgc cttcagcaga tcttcgtact catcaagctt
     5581 cgccatattt ttctagatca aaagcgttga cgcagaggac gcagaggaaa ggcagaaaga
     5641 gcgcagagga aacgaaaacg attcggggat ctacgttcac cgccttcatt ggcatacggg
     5701 caatgtcgac acatcggaaa gggttgctga ataccatacc gacataccag tttctccagc
     5761 ctttctttgc gtcctctctt gtttttcctc tgcgccctct gcgtcaacgc tcttgactta
     5821 gatagttcag aaacaagcgc ggatggtcgc gcccaaggcg tcgcggatgt cgggatcgtc
     5881 ggtgatcggc ttgatcagcg tgacgtggca ggcggcaacg ggctccgcgc cctgcgcaat
     5941 gagcgagccg gcgtagatca gcatccgcgt cgagatacct tcttccaggc cgtgaccctt
     6001 gaggttgcgc gcgcgctcgg cgactgcgac cagcttggcg gccaggtccg ggccgatgcc
     6061 ggcttcgtgg ctgacgatct cgatctcgat gtcgcgcgcg ggaaaaccga aatccagcgc
     6121 gccgaaacgt tgcttggtgg actgcttgag gtccttgagg atgctctggt agccggggtt
     6181 gtaggaaatc acgagctgga aatccggatg cgccgagacc agctcgttct tcttctccag
     6241 cggcaacacg cggcgcgcat cggttagcgg gtggatcact acggtggtgt cctggcgcgc
     6301 ctcgaccact tcgtccaggt agcagatagc accatagcgc actgccagcg tcagcgggcc
     6361 gtcctgccaa cgcgtgccgt cggcgtccag caaataacgc ccgacaagat ccgaggccgt
     6421 catgtcctcg ttgcaggcaa gcgtgatcag cggcttgttc agcttccatg ccatgtattc
     6481 gacgaaacgg gtcttgccgc agccggtcgg gcctttgagc atcatcggca tgcgcacggc
     6541 gtaggccgcc tcgaacatct gcacctcgtc ggcgactgca cggtagtacg gttcggcatc
     6601 gagccggtac tgcttgacga taccctccgc gggcgacagg ttgcgggaat cgttcgggtt
     6661 catggcctat ttccttcgct ggaccggcat ggcgccggtc aatagatttc tcgccgccga
     6721 ccaagcgttg aacatcgcgg tcttgagcgc ggcggcgtca tttgcaagga tacgcgccca
     6781 cgcgccgctc cggttgggca ggagcgcagc cccggcgtat accccaggca ataccagcgc
     6841 ttcgcgcatg gcggcaacga tttccgtggc cggcttcgcg ccggtcacaa tgaacagggt
     6901 gccctgcgcg gtcgaggctc cggcgatgcc ggcgagacgc cgcgccaatt gactgccatc
     6961 gacgcgaaag cggtcgcaaa ccagaagctt tcccgaggga tcctcgaagc gggtttcgga
     7021 tcgcagccag tcgaacacac cgtcgccacc cttgggatcg tgcagcaaga ccgaatcgca
     7081 gaggatgacg ctggactccg gatgcacgcg cacccgcacg cggctatcca gccgggcgcg
     7141 cgggaacagg atggccggat cgggaaggta ttccaggaac gaaccctcgc gtgcctggat
     7201 ttccaggcgc tgcatggccg attcctcctc catgccgtgg acgatcgtaa aagcacctga
     7261 cgtgacgtgc gcggcggcac cctcgccggc ctcgatgcgc aggcgcaggt cgtcgccctc
     7321 aaaaatcccc ccggagcagg actgcaggta caaggtcggc atgccggccg gatcgccggg
     7381 cgaacgcagc gcgcggccga gatggaatgg ataaccgacg cgctggcggc gcacataagt
     7441 cgcacctccg caatcgcggg cgaactcgat gtccgcgtgg atctcttccg gccgttccgc
     7501 cgcaagcgcc gtgccggcaa gatcgaaggg cttcaccggt cgaacaacac ctgcgcagcg
     7561 atcgcgtcca cgacctggtc cacgccttcg ccggtgccac agttggtagt ctgcacggga
     7621 cggccgccgc gcacctcgtt ggcttcgcgg atcatgcgct cgaggtccac gttgacgtaa
     7681 ggcgcgaggt cggtcttgtt gatcaccagc aggtcgcact gcaggacgcc gaggccgcgt
     7741 ttgcgcggaa tgtcgtctcc gccggcgacg tcgatcacga agatccagta gtccaccagg
     7801 tccagcgaaa acgtcgaggc gaggttgtcg ccgccggatt cgatcaggat caggtcgagc
     7861 ttttcgtact tggcatcgag ctcgtcggcc gcctggatgt tcatggtcgg atcttcacgg
     7921 atcgcggtgt gcgggcaagc cccggtttcc accgcaagca cccgcgccgg gtcgatcagc
     7981 ccgctgcggc ggacgcgctc cgcatcctct ttggtcgcga ggtcgttggt gatgacggcg
     8041 aggttggtgc cgcgggaaat gaagcgcggg atcaactgct ccaggagccg cgtcttaccg
     8101 gatccgaccg gaccgccgat
//
LOCUS       DASQOP010000303        11408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1166764, whole genome shotgun sequence.
ACCESSION   DASQOP010000303 DASQOP010000000
VERSION     DASQOP010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11408
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166764"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1665)
                     /gene="ilvB"
                     /locus_tag="VE030_12150"
     CDS             complement(<1..1665)
                     /gene="ilvB"
                     /locus_tag="VE030_12150"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HZF24205.1"
                     /translation="MELTAGVTTELSGAEITIRCLQEEGVEFIFGYPGGAVLPLYDEL
                     FKQEKVKHILVRHEQAAVHGADAYSRSTGKVGVVLVTSGPGVTNAVTGIATAHMDSIP
                     MVIVTGQVPTHAIGQDAFQEVDTVGITRPCVKHNFLVKDIKDLAVTMKKAFFLAATGR
                     PGPVVVDIPKDVQTTKTAYFYPQSVTMRSYNPVIKGHSGQIKKALQLLMEAKRPMIYT
                     GGGIVLGNASAELTQLVRTLGFPCTNTLMGLGGYPASDPQFVGMLGMHGTYEANMAMQ
                     NCDVLLAIGARFDDRVIGNPKHFFQEDRRIIHVDIDPSSISKRVRVDVPIVGDVREVL
                     QEMNRQLAAAKERPDPAAVKAWWTQIEQWRSRDCLKYDRASKIIKPQFVMEKLYELTK
                     DMDLFLTSDVGQHQMWAAQFYKFEKPRRWINSGGLGTMGVGLPYALGVQLAYPDATVA
                     CVTGEASIQMCIQELSTAKQYRLPVKIVNLNNRYMGMVRQWQQFFHGNRYAESYMDAL
                     PDFVKLTESYGHVGMRIDKPEDVEPALKEAFGRKNDLVFMDFITDQT"
     gene            complement(1806..2621)
                     /locus_tag="VE030_12155"
     CDS             complement(1806..2621)
                     /locus_tag="VE030_12155"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HZF24206.1"
                     /translation="METALRKPSPGMLERVIETVKDVAKEEVMPRYLKVAHQRKSDGS
                     LFTEADIAAQEALSHRLPEIYPGPIVAEEMTPEQQAEYWLAGAEGLWCVDPIDGTSNF
                     VNGLPYFAVSVALMKKGRSVLGVVYDPVADEVFYAEKGGGAFLNGERLPIKERAPHLR
                     SAMAQVDFKRLPERLARELVAAPPYSSQRNFGASTLEWCYVAAGRFDVYLHGGQKLWD
                     YAAGSLILEEAGGLMSSLTQDDFWADTLWQRSAIAARDPGLFDAWKRWIRARQ"
     gene            complement(2606..3157)
                     /locus_tag="VE030_12160"
     CDS             complement(2606..3157)
                     /locus_tag="VE030_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007746699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZF24207.1"
                     /translation="MGAQPPLYDFGWKAPDFDLPGTDGKRHTLAETRGPKGLLVMFIC
                     NHCPYVKAVRDRIIRDCAELKALGIGSVAISANDPNDYPEDSFENMQRVAHDFKFPFP
                     YLFDETQEVAHAYDAVCTPDFFGFDKDLGLQYRGRLDASRTAPVANARRELFDAMKAV
                     AETGRGPVDQIASMGCSIKWKRR"
     gene            3259..4029
                     /locus_tag="VE030_12165"
     CDS             3259..4029
                     /locus_tag="VE030_12165"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010117098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HZF24208.1"
                     /translation="MNKELHKKPAARSGARFYFSGTLGNGSEVRLPAEAAHHAARVLR
                     LAVGEPVTLFDGRGGEFEARITRMDRGEVTIKTGAHVAVEREAPLEILLAQGLSSGDR
                     MDLTLQKSVELGVAAIQPVATERSVVKLRDERAARRAEHWQNLVISACEQCGRNRVPP
                     VAPLLGFGEWLGQLSQPGNELRLMLSPHAAVSLRELAPARGRVILLAGPEGGLAPGEA
                     QAAQSRGFAAVRLGPRVLRTETAALAALAAIQALWGDF"
     gene            4359..5126
                     /locus_tag="VE030_12170"
     CDS             4359..5126
                     /locus_tag="VE030_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HZF24209.1"
                     /translation="MEIRNSTFIVTGGASGLGGATVEMIVAGGGNTIIADISRAQGEA
                     LAAKLGKHARFVECDVTREDHGKAAVDAALKEFGGLHGLVNCAGIAVGEKTVGKDGPH
                     ALASFAKVININLIGTFNMIRLAADAMSKGPPNTAGERGVIVNTASVAAFDGQIGQAA
                     YSASKGGIVGMTLPIARDLSRNGIRVVTIAPGIFETPMLKGMPQEIQDALGKMVPFPP
                     RLGRPGEYAALVGHIVQNEMLNGEVIRLDGAIRMQPK"
     gene            5151..6470
                     /gene="argA"
                     /locus_tag="VE030_12175"
     CDS             5151..6470
                     /gene="argA"
                     /locus_tag="VE030_12175"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HZF24210.1"
                     /translation="MQPAQQAQFVQWFRAAAPYVHAFGGRTFVIAFGGEVVADGRFIA
                     LSHDLNLLASLGVRLVLVHGARPQIDAQIKAAKLKTQYVRGMRVTEERTLACVKQASG
                     QLRVEIEALLSMGLPNSPMANADIRVASGNFVTARPRGVVDGVDMLYTGEVRKVNAGA
                     INTRLDDGELVLLSPLGYSPTGEIFNLTLEDVAAETAIALGAEKLVFLMDEPGLKGKG
                     GKLMRELTVSDAEKLLRKDHLLSDDAGLYLPHAIKACKGGTERVHLVSRHTDGALLLE
                     VFTHDGVGSMVTRDPLEQLRDATIEDAGGILKLIEPLEADGTLIKRPRELLEMEINRF
                     CVLEHDHVIVGCAALYPFPDEKSAELACLVVHPDYRDAGAGEQLLKWIEEKAKKQRIK
                     RLFVLTTRAAHWFIEHGFAEAGVDKLPKQKQALYNYQRRSKVLVKPI"
     gene            6608..6883
                     /locus_tag="VE030_12180"
     CDS             6608..6883
                     /locus_tag="VE030_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006401987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HZF24211.1"
                     /translation="MARTVKCVKLGRQGEGLDFPPFPGDLGKRIFENVSKEAWKGWLE
                     HQKMLVNENRLSLADPKARKYLLEQTEKYFFGAGADAAAGYVPPSKG"
     gene            6894..9134
                     /gene="ppk1"
                     /locus_tag="VE030_12185"
     CDS             6894..9134
                     /gene="ppk1"
                     /locus_tag="VE030_12185"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HZF24212.1"
                     /translation="MRRPAGAAHPFPVRCRSPGSRKISKGAHIKAESADRAATLAPEK
                     AAKKRAALAADKSGKTKRSNPQLYLNRELAQLEFNRRVLAQAEDADVPLLERLRFLCI
                     VSNNLDEFFEIRVAGLKAQIELGADTTGPDGATPSQAFKEVSSRTHELVARQYQLWNE
                     GLLPQLAREGIRFLRRRDWSDAQKTWIKAFFLREIMPMMTPIGLDPAHPFPRILNKSL
                     NFAVQLSGKDAFGRNSGFALVQAPRVLPRVIRLPHEIAGCDFGFVFLSSVIHAHVGEL
                     FTGMEVQGCYQFRVTRNSDLFVDEEEVKNLRLALQGELPQRHFGDAVRLEVADLCSPE
                     ISDFLLAQFNLSRDDLYQVNGPVNLVRLMQVPDWVDRSDLKFPPFVPGIPAALTRRPD
                     IFAAIRKGDILLHHPFQSFKPVISFIQQAAADPHVVAIKQTVYRTGANSELMQTLIDA
                     ARSGKEVTVVVELMARFDEEANINWAARLEEVGAHVVYGVVGLKTHAKMAMVVRREDD
                     RLRRYVHLGTGNYHARTAMQYTDFGLFTCHEEICADVNDVFMQLTGLGKAGKLRHLWQ
                     SPFTLHSQALSKIRREAEQARAGKPAGIIAKMNALLEPDIIAALYEASMAGVQVDLIV
                     RGVCALRPGVPGLSDNIRVRSIIGRFLEHHRVFHFQSSNEVFLSSADWMDRNFFRRIE
                     VCFPVLDDKLKKRVIDESLKTYLADNVQAWEMDGDGRFKRRKPERGKGVAAQAVLLEK
                     LASSAS"
     gene            9179..10699
                     /locus_tag="VE030_12190"
     CDS             9179..10699
                     /locus_tag="VE030_12190"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HZF24213.1"
                     /translation="MSQEIEIKLSVPEEHALRLWEVLAHHPHDKPVTHRLFSAYYDTP
                     ERALKAAGAALRLRREGGRWIQTVKSAGHVTGGLHQRAEHEVEVAAQLPSFPAVAEAG
                     FGDLVADRRLREALTVAFTTEINRTSTLLRPAAGKVVEVSLDRGVIAAGDRREPICEI
                     ELELKSGPPRSLFDLALEIAAALPVRLDNRSKAERGYALAANLQPAPVRAAPAALTAQ
                     MTVEEAFVALVSQCLAHIQANEDGLLQGRDPEYLHQARVGLRRLRSVFRVFGAAVARE
                     PFAALLEQLKTLGQTLGIARDWDVFVNEILTHAVREVTDHPGIADLRKRAQAARRRAG
                     HAARTAVAAPEYTGMLLRLSATLNAGSWRGHNAHAAAALPQFAAAAMARQHSRVLKRG
                     RRIEKLAFGDLHRLRIAVKRLRYSAEFFLPLAPKKARNYVRASSELQDLLGRFNDNAL
                     AWRLLDTLGAQESSPAYQQAIGYVRGWSAAEAERCCNQLADAWKKFMKTQPWWVGI"
     gene            10730..11182
                     /locus_tag="VE030_12195"
     CDS             10730..11182
                     /locus_tag="VE030_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004556863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZF24214.1"
                     /translation="MDLILWRHAEAVDGQPDLARELTPKGLKQAKAIAQWLSSRLPRQ
                     TRIIVSPAERTQQTAAALSDEFEIDRQLAPGAPPTAVLAAAGWPGHGGAVVVVGHQPT
                     LGMAAALLIAGEPMPWSIKKGAIWWLSRRVRGEHAQLVLRAVISPDLL"
BASE COUNT         2110 a         3571 c         3709 g         2018 t
ORIGIN      
        1 ggtctggtcg gtgatgaagt ccatgaaaac gaggtcgttc ttgcggccga acgcctcttt
       61 gagcgccggc tcgacgtcct ccggcttgtc gatgcgcatg cccacgtggc cgtaggattc
      121 ggtcagcttg acgaagtccg gcagcgcgtc catgtaggac tcggcgtagc ggttgccgtg
      181 gaagaactgc tgccactggc gcaccatgcc catgtaacgg ttgttgaggt tgacgatctt
      241 caccggcagg cggtactgct tggcggtgga tagttcctgg atgcacatct ggatgctcgc
      301 ctcgccggtc acgcaggcca cggtcgcgtc cggatacgcg agctgaacgc cgagcgcgta
      361 cggcaggccc acgcccatcg tgcccaggcc gccggaattg atccagcggc gcggcttctc
      421 gaacttgtag aactgcgccg cccacatctg gtgctggccc acgtcggagg tcaggaacag
      481 gtccatgtcc ttggtcagtt cgtagagctt ttccatcacg aactgcggct tgatgatttt
      541 cgaggcgcgg tcgtatttca ggcaatcgcg gctcctccac tgctcgatct gcgtccacca
      601 ggccttgacc gcggctgggt ccgggcgctc cttggcggca gcgagctggc gattcatctc
      661 ctgcagcact tccctgacgt caccgacgat cggcacgtcc acccgcacgc gcttggagat
      721 cgaagagggg tcgatgtcga cgtggatgat ccggcggtct tcctggaaga agtgtttcgg
      781 gttgccgatc acgcggtcgt cgaagcgcgc gccgatcgcg agcagcacgt cgcagttctg
      841 catcgccatg ttggcttcat aggtgccgtg cataccgagc atgccgacga actgcggatc
      901 gctggcggga tagccgccca gacccatgag ggtgttggta caggggaaac cgagcgtgcg
      961 caccaattgc gtcaattcgg cggacgcgtt gccgagcacg atgccgccgc cggtgtagat
     1021 catcggccgc ttggcttcca tgagcagctg cagcgccttc ttgatctgcc cgctgtgtcc
     1081 cttgatcacc gggttgtacg agcgcatcgt caccgactgc ggatagaagt acgcggtctt
     1141 ggtagtctgc acgtccttgg ggatgtcgac caccaccggg ccggggcgcc ctgtcgccgc
     1201 aagaaaaaag gccttcttca tggtgaccgc gaggtccttg atgtccttca ccaggaagtt
     1261 gtgcttcacg cacggccggg tgatcccgac ggtgtccact tcctggaagg cgtcctggcc
     1321 gatggcgtgc gtcggcacct ggccggtgac gatcaccatc ggaatggaat ccatgtgagc
     1381 agtggcaatg ccggtcaccg cgttggtcac gccgggtccg gaagtgacca gcaccacgcc
     1441 cactttcccg gtggaccggg aataggcatc ggctccgtgc accgccgcct gctcgtggcg
     1501 taccagaatg tgcttgacct tttcctgctt gaacaactcg tcgtacaggg gaaggacggc
     1561 accgcccggg taaccgaaaa tgaactcgac gccctcttcc tgaagacagc gaatcgtgat
     1621 ttcggccccg gacaattcgg tggtaacgcc ggcggtcaat tccatcgaaa taccctaagt
     1681 ggagaaaatt gctgcgaaaa cttgccaaga taccggcaac gtgaaggccg gtcaaggata
     1741 ggttcccgcg ccgcaacggg gccgcaatct aatcgccccg acggggccgg cgacgcgttt
     1801 gcggactact ggcgcgcgcg gatccagcgc ttccaggcgt cgaacaggcc gggatcgcgc
     1861 gccgcgatcg cggaacgctg ccacagcgtg tcggcccaaa agtcatcctg ggtgaggctg
     1921 gacatcagcc ctccggcttc ctccaggatc agcgaaccgg cggcgtaatc ccagagcttc
     1981 tgcccgccgt ggagatagac gtcgaagcgc ccggcggcga cgtagcacca ttccagcgtg
     2041 ctggcgccga aattgcgctg cgaggaataa ggcggtgcag cgaccagttc gcgcgcaagc
     2101 cgttcgggca ggcgcttgaa gtccacttgt gccatggcgc tgcgcaagtg cggcgcgcgc
     2161 tccttgatcg gcaggcgctc gccgttgagg aaggcgccgc cacccttctc ggcgtagaac
     2221 acctcgtcgg cgaccggatc gtagaccacg cccagcacgc tcctgccctt cttcatcagc
     2281 gccaccgaca cggcgaaata gggcaggccg ttgacgaaat tcgaggtgcc gtctatcggg
     2341 tcgacgcacc acagcccctc ggcaccggcc agccagtatt ctgcttgttg ctccggggtc
     2401 atttcctcgg caacgatagg cccgggatag atttccggaa gccggtggga cagcgcctcc
     2461 tgggcggcga tatcggcttc ggtgaacagg ctgccgtccg acttgcgctg atgcgccacc
     2521 ttcaggtaac gcggcatcac ctcttccttc gccacgtctt tcaccgtctc gatcacgcgc
     2581 tcgagcatcc cgggtgacgg tttcctcagc gccgtttcca tttgatcgaa caacccatac
     2641 tggctatttg atccaccgga ccgcgcccgg tttccgccac cgccttcatg gcgtcgaata
     2701 attcgcggcg tgcgtttgcc accggcgcgg tgcgcgaggc gtcgagcctg ccccggtact
     2761 gaagtcccaa gtccttgtcg aaaccgaaaa agtccggcgt gcacacggcg tcgtaggcgt
     2821 gcgcgacctc ctgggtctcg tcgaagaggt agggaaacgg gaacttgaag tcgtgagcaa
     2881 cgcgctgcat gttttcgaag gagtcctcgg gataatcgtt tggatcgttc gcactgatcg
     2941 ccaccgagcc gatgcccagc gccttgagtt cggcgcagtc gcggatgatg cgatcgcgca
     3001 cggccttgac gtagggacag tggttacaaa tgaacatcac cagcagcccc ttgggtccgc
     3061 gtgtttcggc cagcgtgtgg cgtttgccgt cggtgcctgg caggtcaaag tcgggcgcct
     3121 tccagccgaa atcgtacagc ggtggttgcg cgcccatcgc ctacgcctcc aaggaattgc
     3181 tagcgccggc gcgcgctgtt gcgggatgcc gcgcagaaat cctgtgacca caatcttgtt
     3241 gcatggcaaa atcttaacat gaataaggag ttgcataaga aacctgccgc gagatccggt
     3301 gcccggttct acttttccgg cacgcttggc aacggctcgg aagtgcgctt gcccgcagaa
     3361 gccgcgcatc acgcggctcg cgtactgcgt cttgcggtgg gcgaaccggt cacgctgttc
     3421 gacggacgcg gcggcgagtt cgaggcgcgc attacccgca tggaccgggg cgaggtgacg
     3481 atcaagaccg gcgcgcacgt ggccgtcgag cgcgaagcgc cgctcgaaat cctgctggcg
     3541 caagggctct cgagcgggga tcgcatggac ctgacgctgc agaagtcggt ggaactgggc
     3601 gtcgcggcaa tccagccggt cgccaccgag cgcagcgtcg tgaagctgcg cgacgagcgc
     3661 gcggcgcggc gcgccgagca ctggcaaaac ctggtgatct ccgcctgcga gcagtgcggc
     3721 cgtaaccgcg ttccgccggt cgcgccgttg ctgggtttcg gcgagtggtt ggggcaactg
     3781 agccagcccg gtaacgaact gcgcctgatg ctgtcgccgc atgcggctgt gtcgctacgc
     3841 gaactcgcgc cggcgcgcgg ccgggtgatt ctgctcgccg gtccggaagg cggcctcgcc
     3901 ccgggcgagg cgcaggcggc gcagtcgcgc ggattcgcgg cggtgcgtct cggcccgagg
     3961 gtgctgcgca ccgagaccgc ggcgctcgct gcactggccg cgatccaggc gctgtggggc
     4021 gatttctgat cctacctgca aaccaaaacc aggctcacca cgaagacagg aagaacacga
     4081 agaaggacca aatgtgaatg actcgtcaaa ttcaccatgg tggacctatt agtgtttctg
     4141 ggtgtgacgt cgacaagtgc gaatatccga ggcatttcga taatctggag tcggctgaga
     4201 gtccgcttgc tcccagttga ccatagaata ttttcttcgt gtttcttcgt gtcttcgtgg
     4261 tgagatttgc ggttttcccg gcgttgacgt tttcgcgaaa aagccgccag cccttatact
     4321 gtgcggcttt cacgacttga ccggcaacag gattaacagt ggaaatcagg aacagcacct
     4381 tcatcgtcac cggcggagcc tcgggtctgg gcggcgccac cgtggaaatg atcgttgccg
     4441 gcggcggcaa cacgatcatc gccgacatat cccgggcgca gggcgaagcg ctggccgcga
     4501 aactcggcaa gcacgcgcgt ttcgtcgaat gcgacgtcac ccgcgaggat cacggcaagg
     4561 ccgcggtgga cgccgcgctc aaggagttcg gcggcctgca cggactggtg aactgcgccg
     4621 gcattgcggt gggagaaaaa actgtcggca aggatggtcc gcatgcgctc gccagcttcg
     4681 ccaaggtgat caacatcaac ctgatcggca ctttcaacat gatccgcctt gccgcggacg
     4741 cgatgtcgaa aggcccgccg aacactgccg gggagcgcgg cgtcatcgtc aacaccgcat
     4801 cggttgccgc cttcgacgga cagatcggcc aggcggcgta ctccgcctcc aagggcggca
     4861 ttgtcggcat gacgctgccg atcgcgcgcg atctgtcgcg caatggcatc cgcgtggtca
     4921 ccatcgcgcc gggtattttc gagaccccga tgctcaaagg catgccgcag gaaatacagg
     4981 acgcgctcgg caagatggtg cctttccccc cgcggctggg aaggccgggc gagtacgcgg
     5041 ccctggtcgg acatatcgtt cagaacgaga tgctcaacgg cgaggtgatc cgcctggacg
     5101 gcgcgatccg gatgcaacca aaataaatcc cgtcccaagc cacgcccgcc atgcagcccg
     5161 cccagcaagc ccagtttgtg cagtggttcc gcgccgccgc gccgtacgtg cacgcgttcg
     5221 gcggacgcac gttcgtcatt gcctttggcg gcgaagtggt ggcggacggc cgcttcatcg
     5281 cgctttcgca cgacctgaat ctgctggcga gcctcggcgt gcgcctggtg ctggtgcatg
     5341 gcgcgcgccc gcagatcgat gcacagatca aagccgccaa actgaagacg cagtacgtgc
     5401 gcggcatgcg ggtcaccgag gagaggacgc tggcctgcgt gaagcaggcc agcggtcaat
     5461 tgcgtgtgga gatcgaggcg ctgctgtcga tgggcctgcc gaactcgccg atggccaacg
     5521 ccgatatccg cgtggcctcc ggcaacttcg tcaccgcgcg cccgcgcggc gtggtggacg
     5581 gcgtggacat gctctacacc ggagaggtgc gcaaggtgaa cgcgggtgcg atcaacaccc
     5641 gcctcgatga cggcgaactg gtgctgctgt ccccgctcgg ctattcgccc accggcgaga
     5701 tctttaatct aacgctggag gacgtggcgg ccgagaccgc gatcgcgctg ggtgccgaga
     5761 aactcgtgtt cctcatggat gagcccgggc tgaagggcaa gggaggcaag ctgatgcgcg
     5821 agctcacagt gtcggatgcg gagaaactgc tgcgcaagga tcacttgctg tcggacgatg
     5881 ccggcctgta cctgccgcat gcgatcaagg cgtgcaaagg cgggaccgag cgcgtacacc
     5941 tggtgagccg ccacaccgac ggcgcgctgc tgctggaagt gttcacccac gacggcgtgg
     6001 gcagcatggt cacgcgcgac ccgctggaac agctgcgcga tgccaccatc gaggacgcgg
     6061 gcggcattct caagctgatc gagccgctcg aagcggacgg cacgctcatc aagcgcccgc
     6121 gcgaattgct ggagatggaa atcaaccgct tctgcgtgct cgagcacgat cacgtgatcg
     6181 tcggctgcgc cgcgctctac ccttttcccg acgagaagtc ggcggagctc gcctgcctgg
     6241 tcgtgcatcc ggactatcgc gacgccggcg ccggcgagca actgctgaag tggatcgagg
     6301 aaaaggcgaa aaaacagcgg atcaaacgcc tgttcgtgct caccacccgc gccgcgcact
     6361 ggttcatcga gcacggcttt gcggaggccg gcgtcgacaa gctgccgaag cagaagcagg
     6421 cgttgtacaa ctaccagcgg cgttccaaag ttctggtcaa gccgatttga cgtccgtcga
     6481 ccgacgtcat cccagccgct ccccggggcg ggggatataa tcaccgggaa tcttccgcga
     6541 taaggctgtc acatggctgt aacctcatcg cccggaagaa gctataacct gttgatacca
     6601 aaaaaacatg gcaagaacag tgaagtgcgt gaaactcggc cggcagggcg aggggctgga
     6661 tttccctcct tttccgggtg acctgggcaa gcgcatcttc gagaacgttt ccaaggaagc
     6721 gtggaaaggc tggctcgaac accagaaaat gctggtgaac gaaaaccgcc tcagcctcgc
     6781 cgacccgaaa gcgcgcaagt atcttctgga gcagaccgaa aagtactttt tcggtgccgg
     6841 cgccgacgct gctgcgggtt acgttccacc ctcgaagggt tagcaactct gtagtgcgcc
     6901 ggccggcagg cgcggcgcat cccttccctg ttcgctgccg atcgccgggg agccgaaaaa
     6961 ttagcaaggg tgcgcacatc aaggccgagt ccgccgaccg cgcggctacg ctcgcgccgg
     7021 aaaaggcggc gaagaagcgc gccgcgctgg ccgccgacaa gtcgggcaag accaagcgtt
     7081 ccaatccgca gctttacctg aaccgggaac tcgcgcagct cgagttcaac aggagagtac
     7141 ttgcgcaggc tgaagatgcg gacgtgccgc tgctggagcg gctgcgcttc ctgtgcatcg
     7201 tcagcaacaa cctggacgaa tttttcgaga tccgggtggc gggcctgaag gcgcagatcg
     7261 aactcggcgc cgatactacc ggacccgacg gcgcgacccc ttcgcaggct ttcaaggaag
     7321 tgtcttcgcg cacccatgag ctggtcgcac gccagtacca gctgtggaac gagggactgc
     7381 tgccgcaact ggcgcgcgaa ggcatccgct tcctgcgccg ccgcgactgg agcgacgcgc
     7441 agaagacctg gatcaaggcg ttcttcctcc gggagatcat gccgatgatg acgccgatcg
     7501 gactggaccc ggcgcatccg ttcccgcgca tcctcaacaa gagcctcaat ttcgccgtgc
     7561 agctgtccgg caaggatgcg ttcggccgca attccggatt cgccctggtg caggcgccgc
     7621 gcgtcctgcc gcgggtgatc cggcttccgc atgagatcgc cggctgcgat ttcggattcg
     7681 tattcctttc ctcggtgatc cacgcgcacg tcggtgaatt gttcaccggc atggaggtac
     7741 agggctgcta ccagttccgc gttacccgca acagcgatct gttcgtggac gaggaagaag
     7801 tgaagaacct gcgactggcg ctgcagggcg aattgccgca gcgtcacttc ggcgacgcgg
     7861 tgcggctcga agtcgcggat ctgtgctctc cggaaatctc ggactttcta ctggcccagt
     7921 tcaaccttag ccgagacgat ctgtaccagg tcaacggccc ggtgaacctg gtgcggctga
     7981 tgcaggtacc cgactgggtg gaccggtccg acctgaagtt cccgcctttt gttcccggta
     8041 ttccggccgc gcttaccagg cgtccggata ttttcgccgc gatccgaaag ggcgacatcc
     8101 tgctgcatca tcccttccag tcgttcaagc cggtcatcag cttcatccag caggccgccg
     8161 ccgatccgca tgtggtcgcg atcaagcaga ccgtgtatcg caccggcgcc aactccgagc
     8221 tcatgcagac gctgatcgac gcggcgcgca gcggcaagga ggtcaccgtg gtggtggagc
     8281 tgatggcgcg tttcgacgag gaggccaaca tcaactgggc cgcgcgcctg gaggaggtcg
     8341 gcgcgcacgt ggtatacggc gtggtcggac tcaagaccca cgcgaaaatg gcgatggtag
     8401 tgcgccgcga ggacgacagg ctgcgccggt acgtgcacct gggtacgggc aactaccatg
     8461 cgcgtaccgc gatgcagtac accgatttcg gtctgttcac ctgccatgaa gagatctgtg
     8521 cggacgtcaa cgacgtgttc atgcagctca ccggcctggg caaggccggg aagctcaggc
     8581 atctctggca gtcgcccttc acgctgcatt cgcaggcgct gagcaagatc cggcgcgaag
     8641 ccgagcaggc gcgcgcggga aagcccgcag gcatcatcgc caagatgaac gcgctgctcg
     8701 agcccgacat catcgccgcg ctgtacgagg cttcgatggc cggcgtgcag gtggacctga
     8761 tcgtgcgagg tgtctgcgca ttgcgccccg gcgtgcccgg gctttcggac aatatccggg
     8821 tgcgctcgat catcggtcgc tttctcgagc accatcgcgt tttccacttc cagagctcga
     8881 acgaggtgtt tctttccagc gccgactgga tggaccgcaa cttcttccgc cgcatcgagg
     8941 tgtgctttcc ggtgctcgac gacaagctga agaaacgcgt gatcgacgaa tcgctgaaga
     9001 cctatctagc cgacaacgtc caggcctggg aaatggacgg cgacgggcgc ttcaaacgca
     9061 gaaaaccgga acgcggcaag ggcgtggcgg cacaggccgt gctgctggaa aaactcgcct
     9121 ccagcgcgag ctgaggtacc cgagcggctg cgcgctcgca atcgacgcat tcgccgccat
     9181 gtcacaggaa atcgagatca agctgtcggt ccccgaggag cacgcgctcc gcctctggga
     9241 agtgctcgcg caccacccgc atgacaaacc ggtcacgcat cggctgtttt cggcgtacta
     9301 cgacaccccg gagcgcgcat tgaaggcagc cggcgcggca ttgcgcctga ggcgcgaggg
     9361 cgggcgctgg atccagacgg tgaagagcgc agggcacgtc acaggcgggc tgcaccagcg
     9421 tgccgagcac gaagtcgaag ttgccgcgca actgccgagc ttcccggccg ttgccgaggc
     9481 cggtttcggc gacctggtcg ccgacagacg gctgcgcgaa gcgctcacag tcgcgttcac
     9541 caccgagatc aaccgcactt cgacgctgct ccgacccgcg gccggcaagg ttgtcgaggt
     9601 gagtctcgat cggggagtga tcgcagcggg cgaccggcgc gagccgattt gcgaaatcga
     9661 gctggaactc aagtccggac cgccgcgaag cctgttcgac cttgccctgg aaatcgccgc
     9721 cgcgctgccg gtgcgcctcg acaaccgcag caaggcggaa cgcggctacg cgctcgccgc
     9781 gaatctgcag ccggcgccgg tcagggctgc accggcggcg ttgacggcgc agatgacggt
     9841 ggaggaggct ttcgtcgcgc tggtctcgca gtgtctcgcg catatccagg cgaacgaaga
     9901 cggcctgctg cagggccgcg accccgagta tctgcaccag gccagagtgg ggctgcggcg
     9961 tctgcgctcg gtgtttcgcg tgttcggtgc tgccgtcgcc cgggaaccct ttgcggcact
    10021 gctggaacaa ctcaagacgc tcgggcagac actgggcatt gcacgggatt gggatgtgtt
    10081 cgtcaacgag atactgacgc atgcggtgcg cgaggtaacc gatcatcccg ggattgccga
    10141 tctgagaaag cgtgcgcagg cggcgcgccg ccgagcgggc cacgcagcac gcaccgcagt
    10201 tgctgcgccg gagtacaccg gcatgctgtt gcgtctgtcg gcaacgctga atgccgggag
    10261 ctggcgcggc cacaatgcac acgccgcggc cgcgctgccg cagttcgcag ctgccgcgat
    10321 ggcacgccag cattcgcggg tgttaaagcg tggccgccgc atcgaaaagc tggcattcgg
    10381 ggatttgcac cgtttgcgca tagcggtcaa acggctgcgt tacagcgccg agttttttct
    10441 tccgctggcg ccgaaaaagg cgcgaaacta cgttcgcgcg tcgtcagagt tgcaggacct
    10501 gctgggaagg ttcaacgaca atgcgcttgc gtggcggctg ctggacaccc tgggcgcgca
    10561 ggaatcgtcg ccggcatacc agcaggcaat aggctatgtg cgcggctgga gcgccgccga
    10621 ggccgaacgc tgctgcaacc agcttgccga cgcgtggaag aagttcatga agacgcaacc
    10681 atggtgggtg ggaatttgac gcgctggagg aaactgtgag gacgaccaca tggatctgat
    10741 actttggcgt cacgccgaag ccgtggacgg ccagcccgat cttgcccgcg aactcacgcc
    10801 aaaaggcttg aaacaggcaa aagcaatcgc gcaatggctg agttcgcgtc tgccacggca
    10861 gacccggatc atcgtcagcc cggcagagcg cacgcaacag accgccgcgg cgctcagcga
    10921 cgagttcgaa atcgaccgcc agctcgcgcc cggcgcgccg ccgaccgcgg tgctggccgc
    10981 agccggatgg cccgggcacg ggggcgcagt cgtagtggtc ggccatcagc ccactctcgg
    11041 gatggcggca gcgctgctga tcgccggcga gccgatgcca tggagcatca agaaaggcgc
    11101 gatctggtgg ttgagccgcc gcgtgcgcgg ggagcatgcg caactcgtgc tgcgcgcggt
    11161 gatttcgccg gatcttcttt aagcggtcac caaagcctac tgcgaacacc aaacgctcac
    11221 cgcggaggac ataaagaacg cccctctctc cccagccctc tcccccacaa ccgggtggag
    11281 agggtgatgg aaacgcgttg atgcctgatc gtgtagcttt tcggcgccct ctccagcttg
    11341 ctggagaggg cggggggtga ggtgatttcc ttcgcgtcct ctgcggtgag agttgcccta
    11401 ttgatttg
//
LOCUS       DASQOP010000304         2715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2172992, whole genome shotgun sequence.
ACCESSION   DASQOP010000304 DASQOP010000000
VERSION     DASQOP010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2172992"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VE030_12200"
     CDS             complement(<1..397)
                     /locus_tag="VE030_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24215.1"
                     /translation="MVALASEPQSIGKVLDSGFRLFVKAFTSALPLSLAAAAVLAVPN
                     IANVLMGGTEQAQSPVPASTILVLFLVALPIYMILIAAVVYRLGAAAELHEASTGQAL
                     ARGVRCVLSLIGAAILYFLAMAAGLILLVI"
     gene            complement(515..946)
                     /gene="fur"
                     /locus_tag="VE030_12205"
     CDS             complement(515..946)
                     /gene="fur"
                     /locus_tag="VE030_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HZF24216.1"
                     /translation="MTTSHNLKNIGLKATLPRLKILDLFENSDVRHLTAEDVYKMLMK
                     EGMEIGLATIYRVLTQFEQAGILIRHHFESGKAVFELNQGGHHDHLVCIQCGQVEEFY
                     DPEIERRQVKIAKDRGFTIHEHSLQLYADCIKPDCPNRRKS"
     gene            1050..1394
                     /locus_tag="VE030_12210"
     CDS             1050..1394
                     /locus_tag="VE030_12210"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HZF24217.1"
                     /translation="MRLLWLLPALLAGCMLAPHKIDIQQGNYVDQEMVAKLKPDMTRS
                     QVRFILGTPLVADAFHPNRWDYVHLTGKAGEVRRQRKITVVFDGDRLKYIDGDVVTTE
                     NLSRAPTETANP"
     gene            1436..2242
                     /gene="dapB"
                     /locus_tag="VE030_12215"
     CDS             1436..2242
                     /gene="dapB"
                     /locus_tag="VE030_12215"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HZF24218.1"
                     /translation="MTTQRIAIAGSTGRMGRALIDAVVQAGDFQLKAALERPDSPFLG
                     KDAGELVGSPCGVRISADLERSLADCDVLVDFTRPEATLKHLEICGSRGIRMVIGTTG
                     FTQQQQKLIGAASRNVAVVMAPNMSVGVNLTFKLVELTAKVLNEGYDVEIIEAHHRHK
                     VDAPSGTALRMGEVIAHALGRDLAECAVYGREGVTGERKPSTIGFATVRGGDIVGDHT
                     ALFAGTGERVEITHRAASRATFALGALRAARFLAVKKTGLFDMQDVLGLK"
     gene            2379..>2715
                     /locus_tag="VE030_12220"
     CDS             2379..>2715
                     /locus_tag="VE030_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010397230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HZF24219.1"
                     /translation="MPALDRQPAVLALADGTIFRGISIGAQTHTAGEVVFNTAITGYQ
                     EILTDPSYCRQIVTLTYPHIGNVGVNPEDIESGKVFAAGLVIRDLPVLASNFRRSQGL
                     SDYLKVQGVA"
BASE COUNT          515 a          840 c          843 g          517 t
ORIGIN      
        1 gaatgaccag caatatcagg ccggcggcca tcgccaggaa gtagaggatg gctgcgccga
       61 tcagcgataa cacgcagcgg actccgcgtg ccagcgcctg tccggtgctc gcctcgtgca
      121 attcggccgc cgcgccgagc cggtatacca cggcagcgat caggatcatg tagatcggca
      181 gcgccaccag gaagaggacc aaaattgtcg acgcgggtac cgggctctgg gcctgttcgg
      241 tgccgcccat cagtacattg gcgatattcg ggactgctag caccgctgcc gctgccagtg
      301 acagaggaag agcgctcgta aatgccttga cgaacaacct gaaaccgctg tcgagcacct
      361 tgcctatgga ctgcggttca ctcgcgagtg ccaccatgtt tgccccctaa gtgtcgttgc
      421 gatgaaatcc cgtggcacga acgtccgccg ccgctcctgc agtcacgacg aagccggttc
      481 cagattacgg ccaccattcg ccggctgcac agtttcagga tttgcgccgg ttcggacagt
      541 cgggcttgat gcagtcggca tacaactgca aagagtgctc gtggatggtg aagccgcggt
      601 ccttggcgat cttgacctgg cgtcgctcga tttccggatc gtagaattcc tcgacttgtc
      661 cgcactggat gcagacgagg tgatcgtggt ggccgccctg gttgagttcg aacaccgcct
      721 tgccggattc gaaatggtgg cggatcagga tgcccgcctg ctcgaactgg gtgagcaccc
      781 ggtagatggt tgccaacccg atctccatgc cctccttcat cagcatcttg tagacgtcct
      841 cggcggtgag gtggcgtacg tcgctgttct cgaacaggtc gagaatcttc aggcggggca
      901 gtgtggcctt cagtccgatg ttcttgagat tgtgggacgt ggtcatgaca gaactgacag
      961 ggagaggttg gtgaattatc atagcggctt tcaacttact tggaaaccgc gtccggaggc
     1021 tgttgccgcc ggatgacgcg gctctgttca tgcgcttact ctggcttctg cccgccctgc
     1081 tcgccggttg catgctggca ccccacaaga tcgacatcca gcagggaaac tacgtggacc
     1141 aggaaatggt ggcgaagctc aagcccgaca tgacgcgctc gcaggtgcgc ttcatcctcg
     1201 gtacgccgct ggtggccgac gcgtttcatc caaaccgctg ggactacgtg catctgaccg
     1261 gcaaggcggg cgaggtgcgc cggcagcgca agatcaccgt ggtcttcgat ggcgacaggc
     1321 tcaagtacat cgatggcgat gtggtcacca ccgagaacct ctcgcgcgca ccgaccgaga
     1381 ctgccaatcc ctgatccggg gccggtaggc cccgaattca actcgccgac tgccgatgac
     1441 aactcagaga atcgcaatcg cgggcagtac cggccgcatg ggtcgcgcgc tcatcgacgc
     1501 ggtggtacag gccggcgact tccagctcaa ggccgccctg gagcgtccgg acagcccgtt
     1561 tcttggaaag gatgccggtg agctggtggg ctcaccgtgc ggcgtgcgga tttcggccga
     1621 tctcgagcgc tcgcttgccg actgcgacgt gctggtggat ttcacccggc ccgaggcaac
     1681 gctcaagcac ctcgagatct gcggcagccg cggtatccgc atggtcatcg ggaccacggg
     1741 atttacgcag cagcagcaga agctgatcgg cgccgcgtcc cggaatgtcg cggtggtgat
     1801 ggcccccaac atgagcgtcg gggtgaatct gacgttcaag ctggtcgagc tgacggcaaa
     1861 agtcctgaat gagggttacg acgtcgaaat catcgaggcg catcaccgtc acaaggtgga
     1921 cgcgccctcg ggcacggcgc tgcgtatggg agaagtcatt gcacatgctc ttggacgcga
     1981 cctcgccgaa tgcgcagtct atggccgtga aggcgtaacg ggcgaacgca agccctcgac
     2041 cataggcttc gccaccgtgc gcggcggcga catcgtcggc gatcataccg cgctgttcgc
     2101 cggcaccggc gaacgcgtcg aaatcaccca cagagccgca agccgcgcca cgtttgcgct
     2161 cggcgcgctc agagcggccc gtttcttggc cgtgaagaag acagggttgt tcgacatgca
     2221 ggatgtgctc ggcctgaagt agcaacaccg gcggcgcgtt gaaccgtgcc gtcccttcct
     2281 ctataatgcg ctgtcacaat gaaagcggga ggggcgtgcg cctctcccgc tttccatatg
     2341 tagagtaccc gccaaaccgc gcttcccggg agtcttacgt gcctgcgctc gaccgtcagc
     2401 ctgctgtcct cgcgttagcc gacggcacga tattccgggg catttccatc ggtgcgcaga
     2461 cccacaccgc cggcgaagtc gtgttcaata ccgcgatcac cggctaccag gaaattctta
     2521 ccgacccctc gtattgccgg cagatcgtca ccctgaccta tccacacatc ggcaacgtcg
     2581 gcgtcaatcc ggaagacatt gaatccggga aggtctttgc cgcgggattg gtgatccgcg
     2641 acctgccggt gcttgccagc aacttccgcc gtagccaggg cctgtccgac tacctgaagg
     2701 tgcagggcgt tgccg
//
LOCUS       DASQOP010000305         2690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1769977, whole genome shotgun sequence.
ACCESSION   DASQOP010000305 DASQOP010000000
VERSION     DASQOP010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1769977"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VE030_12225"
     CDS             complement(<1..195)
                     /locus_tag="VE030_12225"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZF24220.1"
                     /translation="MLRRQDRETDCVSVEANGSYDVVVIGSGVGGGAVAFTLADTGAR
                     ILILERGERLPREAENWSPEA"
     gene            complement(159..1274)
                     /locus_tag="VE030_12230"
     CDS             complement(159..1274)
                     /locus_tag="VE030_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZF24221.1"
                     /translation="MATLDLINVEKSFGPTKILHDISISLADGEFLVLVGPSGCGKST
                     LMNIIAGLEEPSAGQLRLDGRDITPLPPADRNISMVFQSYALYPNMTVAKNIEFPLAM
                     RKVEKSKRAERVKSVAKLLQIEHLLERKPRQLSGGQRQRVAIGRALAREPRLYLFDEP
                     LSNLDAQLRLDMRTEIKKLHQRLGATIVYVTHDQIEAMTLATRIAVMKGGVLQQLGTP
                     YEVYNRPANIFVAGFMGSPRMNLVNARLMQNSSGLALDIQTGVRTPVRIDLPDMPSAM
                     NAHIGTDVIAGIRAEAVNLARDGMVAGPTERVFAARVEVIEPTGADTLVVLDLGGNEF
                     TVRLEPDVSLKPGQDARFFVDLAKLVCFDAKTERLIA"
     gene            complement(1279..2148)
                     /locus_tag="VE030_12235"
     CDS             complement(1279..2148)
                     /locus_tag="VE030_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HZF24222.1"
                     /translation="MADRRSTGRGADRVILYAVLLCLALLFLLPVYVMVCNSVKPLDE
                     IRGGNLMALPIAWTIEPWLTAWSTAQIGVQPTGLKPYFLNSFLLVVPAVAISTLIGAL
                     NGYILTQFRFRGANLLFAAMLFSVFIPFQIVLIPMAKTLGILGLAGTVKGLVFVNVVY
                     GIGFTTLFFRNYYAAFPHELIRSARMDGADFFGVLVRILLPSSGPIIVVTVIWQFTNI
                     WNEFLFGASFSDYSSFPITVALNNLVNSSTGVKEYNVHFAGAFIAAIPTLVVYLVSGK
                     YFVRGLMAGSVKG"
     gene            complement(2141..>2690)
                     /locus_tag="VE030_12240"
     CDS             complement(2141..>2690)
                     /locus_tag="VE030_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HZF24223.1"
                     /translation="MALSFIVTGTAWKWLLDPGVGLEHTMHNIGWESFAFGWIKDSDM
                     AIYCVVIAAVWQTTGFVMALFLAGLRGVDAEQISAARVDGARTWQIYLHVIIPQLGPV
                     FVSAFVILAHMAIKSYDLVIALTNGGPGRSTWLPSVFMYQYSFTRNEMSVGAASAVMM
                     LAAIGLVVLPYLFSEMRKVENG"
BASE COUNT          542 a          818 c          844 g          486 t
ORIGIN      
        1 tgcctccggg ctccagttct cggcttccct gggcaggcgt tctccgcgct ccaggatcag
       61 gatgcgcgcg ccggtgtcgg caagcgtaaa cgcgaccgct ccgccaccga cgccggagcc
      121 gatgacgacc acgtcgtagg acccatttgc ctcaacgctc acgcaatcag tctctcggtc
      181 ttggcgtcga agcacaccag cttggcgagg tcgacgaaga agcgcgcatc ctgccccggc
      241 ttcagcgaga cgtccggttc caagcgcacc gtgaattcgt tgccgccgag atcgagcacc
      301 accagcgtgt cggccccggt gggttcgatc acttcgacgc gggcagcgaa aacacgctcc
      361 gtcgggcctg caaccatgcc gtcgcgcgcc aggtttacag cctcggcgcg gatgccggcg
      421 atcacgtcag tgccgatgtg ggcgttcatg gcggacggca tgtcgggcaa atcgatccgc
      481 acaggcgtgc gcaccccggt ctggatgtca agcgctaatc cactgctgtt ctgcatcagt
      541 cgcgcattga ccagattcat gcgcggtgag cccatgaatc ccgcaacaaa gatgttggcg
      601 ggccggttgt atacctcgta gggggtgccg agttgctgta gcacgccgcc tttcatgacg
      661 gcaatgcgcg tggcgagcgt catggcttct atctggtcgt gcgtgacgta gacgatggtc
      721 gcgcccaggc gctggtgcag cttcttgatt tcggtacgca tgtccagccg cagctgggcg
      781 tcgaggttgg acaagggctc gtcgaacaga tacagccgtg gctcgcgcgc cagggcgcgt
      841 ccgatggcga cgcgctggcg ctggccgccc gatagctggc gcggcttgcg ttcgagcagg
      901 tgctcgatct gcagcagctt ggcgacgctc ttgacacgct ccgcacgctt gctcttttcg
      961 accttgcgca tcgctagcgg gaattcgatg ttcttggcca ccgtcatatt cgggtaaagc
     1021 gcgtaggact ggaacaccat agagatgttg cgatcggccg gcggcaacgg cgtgatgtcg
     1081 cggccgtcca gtcgcaactg gcctgcggag ggttcctcca ggccggcgat gatgttcatc
     1141 aacgtgctct tcccgcagcc cgacgggccg accagcacca ggaattcgcc gtcggccagg
     1201 ctgatggaaa tgtcgtggag gatcttggtc gggccgaagc tcttctccac attgatcaga
     1261 tccagcgtag ccatggagtc accctttgac ggaacccgcc atcaagccgc ggacgaaata
     1321 ttttccggag accaggtaga cgaccagcgt cgggatggcc gcgatgaaag cgccggcgaa
     1381 atggacgttg tactccttga ccccggtgga actgttgacc agattgttca gcgccacggt
     1441 gatcgggaac gaactgtaat cgctgaatga agcgccgaac aggaattcgt tccagatgtt
     1501 ggtgaattgc cagatgaccg tgaccacgat gatcggcccg ctgctgggca gcaggatgcg
     1561 aaccagcact ccaaagaagt ccgccccgtc cattcgcgcc gacctgatga gctcgtgcgg
     1621 gaacgcggcg tagtagttgc ggaagaacaa ggtcgtgaaa ccgatcccgt agaccacatt
     1681 gacgaacacc aggcctttca cggtgcccgc cagaccgagg atgccgagtg tcttcgccat
     1741 cgggataagc acgatctgga acggaatgaa gacggaaaac agcatggccg cgaacagcag
     1801 gttggcgccg cggaatctga actgggtgag gatatagccg ttgagtgccc cgatcagcgt
     1861 cgagattgcc accgccggca caaccagcag gaacgagttg agaaagtagg gcttcagtcc
     1921 ggtgggctgt acgccgatct gcgccgtgct ccaggcggtg agccagggct cgatggtcca
     1981 ggcaatcggc agtgccatga ggttgccgcc gcggatttca tccagcggct tgacggaatt
     2041 gcacaccatc acatagaccg gcaacagaaa tagcagcgcc agacacagca ggactgcgta
     2101 aagaatgacg cgatcggctc cccggcctgt cgatcggcgg tcagccattt tccaccttgc
     2161 gcatctcgct gaagaggtag ggcagcacaa ccagtccgat cgccgcgagc atcatgaccg
     2221 cgctcgcggc accgacggac atctcgttgc gggtgaagct gtactgatac atgaaaaccg
     2281 agggcagcca ggtcgagcgg cccggcccgc cgttggtcag cgcgattacc aggtcatacg
     2341 acttgatcgc catgtgcgcc agaatcacga aagccgagac gaataccggg ccgagctgcg
     2401 gaatgatgac gtgcaggtag atctgccagg tacgcgcgcc atcgacgcgc gctgcactga
     2461 tctgttcggc gtccactccc cgcaagccgg cgaggaacaa cgccatcacg aagcctgtgg
     2521 tctgccatac tgcggcgatg acgacgcaat agatcgccat gtcgctgtcc ttgatccagc
     2581 cgaaggcgaa gctttcccag ccaatgttgt gcatcgtatg ctcgaggccg acgccgggat
     2641 cgagcagcca tttccatgcc gtgccggtga cgatgaacga aagcgccatc
//
LOCUS       DASQOP010000306         2916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1964778, whole genome shotgun sequence.
ACCESSION   DASQOP010000306 DASQOP010000000
VERSION     DASQOP010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1964778"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(364..1140)
                     /gene="istB"
                     /locus_tag="VE030_12245"
     CDS             complement(364..1140)
                     /gene="istB"
                     /locus_tag="VE030_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HZF24224.1"
                     /translation="MTDTPKLLLAHHLKTLKLPTVLREFDKVAQQCAAEGLDHVQFLA
                     RLMELELIDRERRMIERRIKAAKFPAVKSLDSFDFKVIPSLNKMQVLELARCEWIDRR
                     ENVIALGPSGTGKTHVALGLGLAACQKGLAVGFITAATLVHELMEARDERRLLRLQKQ
                     LVGHKLLIIDELGFVPLSKTGAELLFELISQRYERGATLITSNLPFDEWTETFGSERL
                     TGALLDRLTHHVSILEMNGDSYRLGQSRARKTQVAAEARP"
     gene            complement(1137..2624)
                     /gene="istA"
                     /locus_tag="VE030_12250"
     CDS             complement(1137..2624)
                     /gene="istA"
                     /locus_tag="VE030_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HZF24225.1"
                     /translation="MELYLKVRLACRDGMSAREAARHFGISRESVKKMLSFSVPPGYR
                     RTAPIKRPKLDEFTEVIDQWLRDDVGQHRKQRHTAKRVFDRLRDEHGFTGGYTIVKDY
                     VRDQLRRRREMFVPLHHAPGHAQADFGEALVVIGGIGQKAHFFALDLPHSDASYIRAY
                     PAATAEAWVDGHVHAFAFFGRVPLSVLYDNDRCLVTRILADGTRKRARLFSGFLSHCL
                     IQDRYGRPGKGNDKGSVEGLVGWARRNFMVPLPRFASWDDFNAWLEEQCRKRHADILR
                     GHTETIGQRLQRDLQAMSELPPAPFDSCEQATGQVSSQSLVRYHTNDYSVPVAYGHRD
                     VWVRGYVDQVVIGCGGEIIARHPRCYDREDMIFDPVHYLPLIEQKIGALDQAAPLAEW
                     DLPAEFQTLRRLMEARMIKAGRREYVQVLRLLESFGMEDLHAAVKTALRMGAVGFDAI
                     KHLVLCQVEKRPPKLDLDVYPYLPRATVGKTSAASYLCLVSRDAA"
BASE COUNT          539 a          932 c          905 g          540 t
ORIGIN      
        1 cgtccgatct gcggtagatg gatcccgacc cgcatgatcc attctccaag atgtggccgg
       61 caccctaggg tgccgccgga gagggatgga tgcaagcgcg tcccggggcc ggaaaccgaa
      121 gcggcgctcg aaggcgccac cgatcgtccg gaggtgccgg ttttctttac acagccttgc
      181 gccgccggcg ggctaggcca agccctgtca acggcggcgg aaaaccgggc cacatgccgg
      241 cgccagagtg gggcagttca gcgcggcgtt ttctgacggt ctgacacggg tgagcgccct
      301 ggcacgcgcg ctccccatat agctggcgtg tgccagggcg ctttggcccg tcagggccaa
      361 tctttacggt cgagcttctg ccgcgacctg ggtcttacgg gcacggctct ggccgaggcg
      421 atagctgtcg ccgttcatct cgaggatgct gacatgatgt gtcagcctgt cgagcagcgc
      481 gccggtcagg cgctcggaac cgaaggtctc ggtccactcg tcgaagggca gattgctggt
      541 aatcagggtg gcgccgcgtt catagcgttg tgagatcagc tcgaacagca actcggcgcc
      601 ggttttgctg aggggcacga aacccagttc gtcgatgatc agcagcttgt ggccgaccag
      661 ctgcttttgc aggcgcaaca gccgccgctc atctcgggcc tccatcaact catggaccaa
      721 ggtggcggcg gtgatgaagc cgacggcgag gcccttctga caggcggcca gccccaggcc
      781 cagcgcgaca tgggttttgc ccgtgccgct cggcccgagg gcgatgacgt tttcccgccg
      841 gtcgatccat tcgcagcggg ccagttccag gacctgcatc ttgttgaggc tggggatcac
      901 cttgaagtcg aagctgtcca ggcttttgac cgcggggaac ttggcggcct tgatccggcg
      961 ctcgatcatc cgccgttcgc ggtcaatcag ctccagttcc atcagtcggg caaggaactg
     1021 aacatggtcc aacccctcgg cagcgcactg ctgggccacc ttgtcgaatt cccgcaggac
     1081 ggtcggcagc ttcagcgtct tgaggtgatg cgccaaaaga agtttggggg tgtcggtcat
     1141 gcggcatccc tcgacaccag acacaggtaa ctggccgccg aggtcttgcc gacagtggca
     1201 cgcggtagat aggggtagac gtcaaggtca agcttcggtg ggcgcttctc cacctggcac
     1261 agcacaaggt gcttgatggc gtcaaaacct accgccccca tgcgcagtgc ggttttgacc
     1321 gcggcatgga ggtcttccat cccgaagctc tccagcaggc ggagaacctg aacgtattcc
     1381 cggcggccgg ccttgatcat ccgcgcttcc atcagccgcc gcagggtttg gaactccgcg
     1441 ggcaggtccc attcggccag tggtgccgcc tggtccagcg cgccgatctt ctgctcgatc
     1501 aggggcaggt agtggactgg gtcgaagatc atgtcctcgc ggtcatagca ccggggatgg
     1561 cgagcgatga tctccccgcc gcagccgatc acgacctgat cgacatagcc ccgtacccag
     1621 acatcgcgat ggccgtaggc gacaggcacc gaataatcgt tggtgtggta gcggaccagc
     1681 gactgcgaac tcacctgtcc tgtcgcctgt tcgcaggaat caaagggggc aggcggtagc
     1741 tctgacatcg cctgcagatc ccgctggagc cgctgtccga tcgtctcggt atggccccgc
     1801 aggatgtccg cgtgacgctt gcggcattgc tcctccagcc aagcgttgaa gtcgtcccac
     1861 gacgcaaaac gcggcagtgg caccatgaag ttgcgacgag cccagccgac cagaccctcg
     1921 acgctgccct tgtcgttgcc tttaccagga cggccgtagc ggtcttggat caggcaatgc
     1981 gacaggaacc cgctgaagag gcgggcgcgt ttgcgtgtgc catccgccag gatgcgcgtc
     2041 accaggcatc tgtcgttgtc atagagcacc gacagcggca cccggccgaa gaaagcaaaa
     2101 gcgtgaacgt ggccgtcgac ccaagcctct gccgttgccg ctggataggc ccgaatgtaa
     2161 ctcgcgtcgc tatgcggcag atcgagcgcg aagaagtggg ctttctgccc gatcccgccg
     2221 atcaccacca gcgcttcgcc gaaatcagcc tgggcatggc caggggcgtg gtgcaacggg
     2281 acaaacatct cccgcctgcg gcgcagctga tcgcgcacgt aatccttgac gatggtgtag
     2341 ccgccggtga acccgtgctc gtctcgaagc cgatcaaaaa cccgcttggc cgtgtggcgc
     2401 tgcttgcggt gctggccaac gtcgtcgcgc agccactggt cgatgacctc ggtgaactcg
     2461 tccagcttgg gccgcttgat cggcgccgtc cgccgatacc ctggcggtac cgaaaagctc
     2521 agcatcttct tcacgctctc gcgcgaaatc ccaaaatgac gtgccgcctc gcgcgcactc
     2581 atcccgtccc gacaggcaag acgcaccttc agatacagtt ccacggtgaa aatgcccccg
     2641 ccctccctgc tgaaaggaag ggcaaaagtg gcagagtttt actccgccag ctgcgggact
     2701 atcccgccgc taccgtggca gacttttcca ccgccgttct caccggcatg cgggcagacc
     2761 gatcaagacc tcccgaaaaa gccgaaccta gccccgtttc ggattcgacc cgctagccct
     2821 agatattgat caacctgggc aacgggatga gccttctgat acgggatctc gagaaatatg
     2881 tgctcgacac tgagacgcac gcgatctttc tgcttt
//
LOCUS       DASQOP010000307         5627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1964840, whole genome shotgun sequence.
ACCESSION   DASQOP010000307 DASQOP010000000
VERSION     DASQOP010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5627
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1964840"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VE030_12255"
     CDS             <1..354
                     /locus_tag="VE030_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24226.1"
                     /translation="HAIDPGPQYAVHFPIFPREQSARLKRLEFRSELKFFQDNGAKEL
                     ALQTVDGIKCKLFRLELDDLELTLFLKPDNLPLQIEVQSAGVKYAVRFLRYDPDQKPD
                     MTLFSVPPGIKIIEP"
     gene            416..1021
                     /locus_tag="VE030_12260"
     CDS             416..1021
                     /locus_tag="VE030_12260"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HZF24227.1"
                     /translation="MDVWLFWKSVSIGVAVAAPVGPMSLLCIHRTLDHGQPAGLTFGA
                     GIAAADLTYAAIAAFGVTTVSSLLLAGTYWIKLIGSLLLLVLGLAIALARPAADSRKT
                     VSGSGLRAFVTAYGLTLANPPTILFFAGIFASVASLARASQSVMFSTGVFAGSMLWWI
                     ILTTLVAKSAALMKPPVMAWINRVSGLVLIGFAAYGLAIMA"
     gene            1095..2285
                     /locus_tag="VE030_12265"
     CDS             1095..2285
                     /locus_tag="VE030_12265"
                     /inference="COORDINATES: protein motif:HMM:NF014324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HZF24228.1"
                     /translation="MRCSLFLIAWSAAAFARADFLLVPESATVAAGGRIEVTLFLPND
                     SAEERSFDLPARLTLRARGRSDAPVVVLEATEAQPAQVRLAPGAFHRARYAGTLPEGM
                     VGEIVLEPVDFQGPPLAFSASAPEVSPSGESPPSAAAQATSAPQIPAPAAALDRDAAR
                     FASAFSPYEPNYFSAGSHGPTNARYQISFKFRFFNPETKTPFLEKIFLGYSQTSIWDL
                     SSSSKPFRDSSYRPSLFFLDDTVSQWPFRGSKLGFQGGFEHESNGKDGTASRSINIAF
                     VRPTLTFPLEGDYFISASPKIYNYLDKQENPDIAEYRGYVDLLVKIGETDGLQFASTL
                     RKGTRSEAYSFQLDVSYPLKRPTFGNLGGYLHLQYFNGYGESLIDYNRRVLPQIRIGL
                     MITR"
     gene            2536..3912
                     /locus_tag="VE030_12270"
     CDS             2536..3912
                     /locus_tag="VE030_12270"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HZF24229.1"
                     /translation="MRWRLRIISAAILASAGWLAAAGEFQLSAPSGGSFTVRDQSEQA
                     YSAPAPTLSAEQLQRFADGRSEFHQRWVVPVNIGGNWGRGPTSNGEVCTDCHAGNGRG
                     RPPEHEDDESVSMLVRLSLPGEDEHGGPRPHPHYGDQLQQQGILGKVPAEGRVTVAWR
                     EHEVELTDGDTVRLRVPEVRISRLAFGPLGADILISPRVAPPVFGMGLLEAIAEETLL
                     EIARRQAALGFNGHPNYVWDAENRVMSLGRFGWKAGQPTLRQQTAAAFLNDMGVTTRV
                     FPQANCPPVQKACNNSPGVGIPELTDRPFESLNFYIRALAVPARRNLDDAGVARGEKL
                     FEQAQCALCHVPEVQTGDYPALPQIARQVIRPYSDLLLHDMGEGLADGRPDYRAGPRD
                     WRTPPLWGIGLSGKVNGNATLLHDGRARNYAEAILWHGGEAAAARDAFRSMPKQEREA
                     LLAFLASL"
     gene            3930..4346
                     /locus_tag="VE030_12275"
     CDS             3930..4346
                     /locus_tag="VE030_12275"
                     /inference="COORDINATES: protein motif:HMM:NF042415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY0301 family protein"
                     /protein_id="HZF24230.1"
                     /translation="MRLVVAAAAALLALNVRAEVACPDSLEVQQQAVMPSEDWTAIYT
                     DPPLRLTGVTIFNGPPSQNRKVKPFSTRSTQGELRIVWRLPESPRNFHLLCTYERTSA
                     NLVTVLPPGVTSCSAVYDRHVSYGRGGYAVKRMVCS"
     gene            4651..5310
                     /locus_tag="VE030_12280"
     CDS             4651..5310
                     /locus_tag="VE030_12280"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HZF24231.1"
                     /translation="MNIDVISDVVCPWCFIGKRHLESALAAWRKEHPGEEQPLVRWHP
                     FQLNPQLPRSGVPRAEYIATKFGGPQRAKEIYARVANAGARAGIAFNFDGITVQPNTL
                     NAHRLIHRAGPDGRQDAMAEALFRAYFLEGADLSSDDTLADIAARAGFDRNEISAYLA
                     GDQDKALIDDQDQHARSLGVEGVPFFIFNRRHAVSGAQPPETILEVMEKAQEELAAAP
                     Q"
     gene            5417..5569
                     /locus_tag="VE030_12285"
     CDS             5417..5569
                     /locus_tag="VE030_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24232.1"
                     /translation="MCFEFEALYWAKLAEEERQKREAKKQAKPVKPAETKPQGRPEYE
                     EDPLPV"
BASE COUNT         1088 a         1790 c         1712 g         1037 t
ORIGIN      
        1 catgcgatcg acccgggtcc acaatacgca gtccactttc cgatctttcc acgggagcag
       61 tcggcgcggc tgaagcgcct cgaattccgc tcggaactca agtttttcca ggacaacggc
      121 gccaaagaac ttgcattgca gaccgtggac gggatcaagt gcaagctctt tcgcctggag
      181 ttggacgacc ttgaattgac gctgttcctg aagccagaca atctgccgct gcagatcgaa
      241 gtacaatcgg ccggcgtcaa atacgcggtg cgctttctca ggtacgaccc agaccagaag
      301 ccggatatga ccctgttcag cgtaccgccc gggatcaaga tcatcgagcc ataattccca
      361 acggcgcgga tgggccgaag gcggcgttat tcgtccggta cgacaagaac agaacatgga
      421 cgtgtggctt ttctggaaat ccgtctccat cggtgtcgcc gttgccgctc ctgtcggccc
      481 gatgagcctg ctgtgcattc atcgcacgct ggaccatgga cagcctgccg gcctgacgtt
      541 cggggccggc atcgcggccg ctgacctcac ttatgcggcg atcgcggcat tcggcgttac
      601 caccgtatct tccctgttgc tggcgggcac ctattggatc aagctgatcg gatcgctcct
      661 gctgctagtg ctgggcctcg caattgccct tgcgcggccg gctgccgact cgcgcaaaac
      721 cgtctccggg tccgggttgc gtgcatttgt caccgcttac ggtctgacgc tcgccaatcc
      781 gccgaccata ctgttcttcg ccggcatttt tgcctcggtc gcgtcgctgg cgagggcctc
      841 gcagtcggtc atgttctcca ccggcgtatt cgccggctcg atgctgtggt ggatcattct
      901 caccacgctg gtcgccaagt cggctgcgtt gatgaagccg ccggtcatgg cctggatcaa
      961 ccgcgtgtcc ggcttggtgc tgatcggttt tgctgcctac ggactggcaa taatggcgtg
     1021 aacgaccgtc acgaaattct tgtgcccagc cgctaaaccc cagtcttgct gacgagaatg
     1081 ccgacgaggc cgatgtgcgc tgttcactgt tcctgattgc ctggtcggcg gcggctttcg
     1141 cccgcgccga ttttctgctc gtacccgaaa gcgcgacagt cgccgccggt ggccggatcg
     1201 aggtgacgct gtttcttccg aacgactctg cagaggaacg cagcttcgac ttgccggcgc
     1261 ggctcacgct gcgcgctcgt ggcagatcag acgcgccggt ggtggtgctc gaggcgaccg
     1321 aggcgcaacc ggctcaagtg cgcctcgcgc ccggcgcatt ccatcgcgcg cgctatgccg
     1381 gcacgctgcc cgagggcatg gtcggcgaga tcgtgctgga gccggtggac ttccagggcc
     1441 cgccgctggc gttcagcgcc agtgcacccg aagtctcgcc ttcaggggaa tcgccgccgt
     1501 cagcggcggc acaggccacg tccgctccgc aaatccctgc ccccgccgca gcactggatc
     1561 gggacgccgc caggtttgct tccgcgtttt cgccttacga gccgaactat ttttcggcgg
     1621 gctcgcacgg gccgaccaat gcgcgctatc aaatcagctt caaattccgc ttcttcaacc
     1681 cggaaaccaa gacgccgttc ctggaaaaga tcttcctcgg ctactcgcag acgtcgatct
     1741 gggatctaag ttcatcctcc aagccgtttc gcgattccag ttatcgcccg agcctgttct
     1801 tcctcgacga cacggtatcg caatggccct tccggggatc gaagctggga ttccagggtg
     1861 gattcgagca cgaatcgaac ggcaaggacg gcaccgcctc gcgttcgatc aacatcgcct
     1921 tcgtgcgacc cacgcttacc tttccgctcg agggagacta tttcatctcg gcgtcgccaa
     1981 agatatacaa ctacctcgac aagcaggaga acccggatat tgccgagtac cgcgggtatg
     2041 tggacttgct ggtaaagatc ggcgaaacgg acggattgca gtttgcctcg acgcttcgca
     2101 agggcacgcg ttctgaagcg tacagcttcc agctggatgt atcctacccg ctgaagcggc
     2161 cgacgttcgg caacctgggt ggctacctgc atttgcagta tttcaacggt tacggtgaaa
     2221 gcctgatcga ttacaaccgg cgcgtgctcc cgcagatccg catcggcctg atgatcacac
     2281 gctgacgtcg aaacgggcgg gcatcgggcg ctatactcat gcatcagaag cagatgccaa
     2341 gcgaaccgcg catctcgaaa ggcgaggtta taacgtgctt cgcttctgga accgtgaggt
     2401 cattgaagat accaaaacag ccgaatgcat cttccgaaac ctaaactcga tgcgataacg
     2461 caagcattgc gtggctccca acaccctctc cgcatcttga cctttcctcc ctctccactt
     2521 ggggagaggg ccggggtgag gtggcgcctt cgaatcatca gcgctgcgat tctggccagc
     2581 gccggctggc tggctgcggc aggggaattt caactgtcgg cgccttcggg cgggtcattc
     2641 acagtgcggg accaatccga gcaggcctac tcggcgcccg cccccacgct cagcgccgag
     2701 cagcttcagc ggtttgccga tggcaggtcg gagttccacc aacgctgggt ggtgccggtc
     2761 aacatcggcg gcaactgggg ccgcggcccg acgtcgaacg gcgaggtctg cactgattgc
     2821 catgccggca atggtcgcgg ccggccaccg gaacacgaag acgatgaatc ggtctcgatg
     2881 ctggtcaggc tgtcgctgcc cggcgaagat gaacacggcg ggccgaggcc gcatccgcat
     2941 tacggcgacc agctccagca acagggcata ctcggcaagg taccggcaga aggccgggtc
     3001 acggtggcct ggcgcgagca cgaggtcgaa ctcacggacg gcgacaccgt acggctgcgt
     3061 gtcccggaag tgcgcatcag ccggctcgcc ttcggtccgc tcggagccga tatcctgatc
     3121 tcgccgcgcg tggccccgcc ggtgttcggc atgggtctgc tcgaggcgat cgcggaggaa
     3181 accctgctcg aaatcgcgcg ccggcaggcg gcgctcggct tcaatgggca tccgaattac
     3241 gtgtgggacg cggagaaccg ggtgatgtct ctggggcgct tcggctggaa agccggacag
     3301 cccacgctca gacagcagac cgccgccgca ttcctgaacg acatgggcgt caccacgcgc
     3361 gtgttcccgc aagccaactg cccgcctgtg cagaaggcct gcaacaacag tccgggagtc
     3421 ggcattccgg aactgaccga ccggccgttc gagtcgctga atttctacat acgcgcgctc
     3481 gcggtgccgg cgcggcgcaa tctcgacgat gccggggtgg cgcgcgggga aaagctgttc
     3541 gaacaggcgc agtgcgcgct ttgccacgta ccggaagtgc aaaccggcga ctacccggca
     3601 ttgccgcaga ttgcccggca ggtgatccgc ccctactccg acctgctgct gcacgacatg
     3661 ggcgaaggac tcgccgacgg ccggcccgat tatcgcgccg ggccgcgcga ctggcgcacg
     3721 ccgccgctgt ggggaatcgg cctcagcggg aaagtcaacg gcaacgcgac gctgttgcac
     3781 gacggccgcg cgcgcaatta tgccgaagcg attctgtggc atggcggcga agctgcggcc
     3841 gcccgcgacg ccttccgcag catgccgaag caggagcgcg aggcgctgct cgcctttctc
     3901 gcatcacttt gacgactgag ggaaccacca tgcgtctcgt tgtcgccgct gcagccgcgc
     3961 tcctcgcact caacgtccgt gccgaggtag cctgtcccga ctcgctcgag gtgcaacagc
     4021 aggccgtcat gccatcggag gactggacgg cgatctacac cgatccgccg ctgcggctca
     4081 ccggcgtgac catcttcaat gggccgccct ctcagaaccg caaggtcaaa cctttttcca
     4141 ccagaagcac gcagggtgaa ttgcgcattg tctggcgcct gccggaaagt ccgcgcaatt
     4201 tccacctgct gtgtacttac gaacgcacct cggccaacct ggtgacggtg ttgccgccgg
     4261 gagtgaccag ctgcagcgcc gtatacgacc gtcacgtctc gtatggccgt ggaggctacg
     4321 cggtgaaaag aatggtgtgc agctagaagc cccccctctc cccgccctct cccgccgggg
     4381 gagagggaga tctaaaatcc gacaatgagg atttgcaaac atgcgtcgca atagacacga
     4441 gttacactgc ctgaagaaga aatttgcaag ttccagggct ccctctcccc gccctctcgc
     4501 gccaggggag agggaggtct agaatccgaa gtggaggttt tgcaaacatg catcggaaca
     4561 gatacaagat tcactgcctg aagaaggaat ttgcaagtcc cagcgctccc tctcccctgg
     4621 tgggagaggg ccggggagag ggggatgtcc atgaacattg atgtaatttc cgacgtggta
     4681 tgcccgtggt gcttcatcgg caagcgtcac ctcgaatccg cactcgccgc gtggcgcaag
     4741 gagcatccag gcgaagaaca acccttggtg cgctggcatc ccttccagct caatccgcag
     4801 ttgccccgca gcggcgtgcc gcgcgccgaa tacatcgcga caaaattcgg cggcccgcag
     4861 cgtgccaagg aaatctacgc cagagtcgcg aatgccggcg cccgtgccgg catcgccttc
     4921 aacttcgacg ggataacggt acagccgaac acgctgaatg cgcacaggct gattcatcgt
     4981 gccggcccgg acggcaggca ggatgcgatg gcggaggcgt tgttccgggc ctatttcctc
     5041 gagggcgctg atctgtcgag cgacgacacg ctcgccgaca tcgccgcgcg cgccggcttc
     5101 gaccgcaacg agatcagcgc ttacctcgcc ggcgaccagg ataaggcact catcgacgac
     5161 caggatcagc atgcgcggtc gcttggcgtc gaaggcgtgc ctttcttcat tttcaaccgg
     5221 cgccatgccg tttccggtgc gcagccgccc gagaccatcc tcgaggtaat ggagaaagcg
     5281 caggaagagc ttgctgcggc gccgcaataa acgctcagat tactgttgat ccgggccggc
     5341 gatggcgcta gcattcggcc aagaataaaa gcaccaggaa ctgcgtgacc cccaacttcg
     5401 acgaaggagg tgcaccatgt gctttgagtt cgaagccctg tactgggcaa aactggccga
     5461 agaagaaagg caaaaaagag aggccaaaaa gcaggcaaaa ccggtgaagc cggcagaaac
     5521 aaaaccgcaa gggcggccgg aatacgagga agacccgctt cccgtctgaa ccaccgcaaa
     5581 ccggtcttgg attgcaaaac cccgcgcgag cggggttttt gtttttc
//
LOCUS       DASQOP010000308         2029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_160217, whole genome shotgun sequence.
ACCESSION   DASQOP010000308 DASQOP010000000
VERSION     DASQOP010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160217"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2029)
                     /locus_tag="VE030_12290"
     CDS             complement(<1..>2029)
                     /locus_tag="VE030_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZF24233.1"
                     /translation="LLDRPTRAALAKIRQMLVGGEALPAALARELEDAIGGDLRNMYG
                     PTETTVWSTTHPVASPTDSVPIGRPIGNTQIYILDAHGQPAPIGVPGEIYVGGAGVAR
                     GYLNRAELTAQRFVPDLFRTTPGATLYRSGDLARRLENGDIEYLGRIDNQVKIRGIRI
                     ELGEIEATLLGHPAVRQAVAMVREDEPGDKRLVAYVVADEGALRGAVHSEESTKRSAE
                     HVSQWRGLWEQTYEQPSNEDAAFNIAGWNSSYTGELVPAEEMREWVDATVERIAARRP
                     GRVLEIGCGTGLLLARLAPACDAYLGTDFSPRALDHVRKLIATRRDLSHVELLQRAAD
                     DFHGIEPRSFDTVIINSVAQYLPSIEYLRAVLKAAVAAVSPGGRIFVGDVRNLPLLKA
                     FHASVQCHRAEGGTRKSQLRHLIENDVELDAELVINPAFFVALKDQDDRISDVEIFLK
                     RGHSQNELTRFRYDAFLHVEATHRPSPPDAWLDWRQERLTLADLKGRLASNPRALGVC
                     GIPNARLVVAVKALDWLASEEGPETLEGFRQALASATDEAVEPELLWTLAEKHGYALE
                     LCYSSTGSDARIDALFRKGDILVPDAVFWGRQANSPAKPWAAYANNPLKVKLVRDLTP
                     RLRKYLGEALPDYMVPADFVILERLPLTPNGKVDRKALPAPGSTVATTAVYV"
BASE COUNT          374 a          690 c          583 g          382 t
ORIGIN      
        1 gcacgtacac ggccgtcgtt gccaccgtgc tgcccggtgc cggcaaggct tttcggtcga
       61 ccttgccatt gggcgtcagc ggcaaccgct cgagtatcac gaaatcagcg ggcaccatgt
      121 agtccggcag cgcttcccca aggtatttgc ggagccgcgg tgtcaaatcg cgcaccagct
      181 tcaccttcag gggattgttc gcgtacgcag cccagggctt cgctggtgaa tttgcttgcc
      241 gcccccagaa gacggcatct ggaaccaaga tatcgccctt gcggaacagg gcatcaatgc
      301 gagcatcgga accggtgctg gagtagcaga gctccagcgc atagccgtgc ttttcggcca
      361 aagtccacaa cagctcaggc tctaccgcct cgtcagtagc cgatgccagg gcctgtctga
      421 agccttccaa cgtctccggc ccttcttcgc tggccagcca atccagcgcc ttcaccgcga
      481 ccaccaagcg agcgttgggg ataccacaaa ctccaagtgc acggggattg gaggccagac
      541 gtcccttcaa gtctgccaag gtcaaccgct cctgccgcca gtccagccac gcatccggcg
      601 gtgacggtct atgcgtggcc tccacatgca gaaacgcgtc gtagcgaaaa cgtgtcagct
      661 cattctgaga gtggccccgc ttcaggaaga tctcaacgtc actgatgcga tcgtcctgat
      721 ccttcagcgc cacaaagaaa gccggattga tcaccagttc agcatcgagt tcgacgtcat
      781 tctcgatcaa atgccttagc tgcgacttcc tggtaccccc ctctgctcga tgacattgga
      841 ccgacgcgtg aaaggctttg agcagcggca gattcctcac atcaccgaca aatatccgcc
      901 cgcccgggct caccgccgcc accgcagcct tcagcacagc cctcaagtac tcgatgctcg
      961 gcaaatactg cgcgacggag ttgatgatca ccgtatcgaa actcctgggc tcgatgccat
     1021 ggaagtcgtc cgccgcacgc tgcaatagct cgacgtggga cagatcccgt cgcgtagcga
     1081 ttaacttgcg aacgtggtca agcgctcttg gggagaaatc cgtccccaga taggcatcac
     1141 aagcaggcgc aagtcgggcc agcaacagcc ctgtcccgca gccaatctcc agcacccgac
     1201 ccggtcgccg cgcggcaatg cgctctaccg tggcatctac ccactcgcgc atctcctctg
     1261 ccgggacgag ctctcccgtg tagctgctgt tccagcctgc gatgttgaac gcggcgtctt
     1321 cattcgaggg ttgctcgtac gtctgctccc acaacccgcg ccattgcgaa acatgctccg
     1381 cgctccgctt ggtactttcc tcactgtgaa ccgcccctct tagcgcacct tcgtccgcca
     1441 ccacgtaagc cacgagccgc ttatcccccg gctcgtcttc ccgaaccatc gccaccgcct
     1501 ggcgcaccgc tggatggccc agcaacgttg cctcgatctc gcccagctcg atgcgaatgc
     1561 cccggatctt gacctggttg tcgatgcggc caaggtattc gatgtcgcca ttctcgagac
     1621 ggcgcgccag atcgccgctg cggtacagcg tggcccctgg tgtggtgcgg aacaggtcgg
     1681 gaacgaagcg ctgcgcggtg agctcggcgc ggttgagata gccgcgcgca acgcccgcgc
     1741 cgccgacata gatttcgccg ggcaccccga tgggggcggg ctgcccgtgc gcatcgagga
     1801 tgtagatctg ggtattgccg atcgggcgtc cgatcgggac cgaatcggtg ggggatgcca
     1861 ccggatgcgt ggtggaccag acggtcgttt ccgtggggcc atacatgttg cgcaggtccc
     1921 cgccaatggc gtcctcgagt tcgcgcgcca gcgccgccgg gagtgcctcg ccgccgacca
     1981 gcatctggcg gatcttcgcc agtgccgccc gggttggtcg atccagcag
//
LOCUS       DASQOP010000309         2659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2977144, whole genome shotgun sequence.
ACCESSION   DASQOP010000309 DASQOP010000000
VERSION     DASQOP010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2977144"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(661..>2659)
                     /gene="mdoH"
                     /locus_tag="VE030_12295"
     CDS             complement(661..>2659)
                     /gene="mdoH"
                     /locus_tag="VE030_12295"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF003956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HZF24234.1"
                     /translation="YIFLGTAALTVTGCYEIYEVLQVGGITALEAMVLVLFVLLFAWV
                     AFSFMSALAGFVVLLFRMRDALGIDPDAPLPVVSRRNAMLLPTYNEEPHRIMARLRAI
                     HESIGETGCASQFDWYVLSDTTDPSIWIEEEKCFLQLRREVGAGQLFYRHRPENTARK
                     SGNIEDWVRRFGAGYDHMIILDADSLMTGDTIVRLAAAMEQHANVALIQTLPIVVNAR
                     TLFARLQQFAGRLYGPLIAAGIAWWHGSEGNYWGHNAVIRVRAFAEDAGLPELRGRKP
                     FGGHILSHDFVEAALMRRAGWAIHMAPTLGGSFEECPPSLLDFAARDRRWCQGNLQHL
                     AVLPARGLHWVSRLHLLTGIGSYLTAPLWLIFLVLGILISLQAQFVRPEYFPKGFSLF
                     PQWPAQDPILAAWVFLGTMGLLIVPKLLAYVLLLTQSRNRRHFGGGLVVFAGILIETF
                     LSGLIAPVMMIFQSTAVGEILLGRDAGWQVQRRDDGGVTRGETIRKYALPTLFGVMMA
                     GTAYAVSLPLLLWMAPVILGLLLAVPIAIVSSTAGSGVAHHQPRLFGTPEQTSPPQVL
                     IRANELANASRETFAGPMHELRNNPDLLDAHVTNLSVKPRNRGQIDPHLAIARAKIED
                     AEIFEDALGYLGPRELFAVLNSRAILEALFKLPMAETHSRP"
BASE COUNT          572 a          805 c          805 g          477 t
ORIGIN      
        1 gccccggcaa atcagccgcc ttgtaacccc gcgcgtggcg gcacgacatc cctgaacgcg
       61 tcgaatccct tccatgcggg ctcgacggga gcaaagcatg cttgtcatga ccggcctccg
      121 ggaccggatc atacggcacc cggcagggtt tgcggagcag agaagcaaaa tttcccgcat
      181 tccagggcgg actggccgct tcggtcgcag aagcgccatc aaaccgtacc tttgttgggc
      241 cgcaattcga gagtcacttg cacaatcaca atgttcggtg ggaaccacaa tgaaaagact
      301 tgtccttgcg tcggcgatat ttttgacgtt tgcgccggca gtcgctctgg ctgaagggcg
      361 tgggggcgat gctgccttgg gtgccctgtc gggtgcggtg gtgttcggcc cggtggggtt
      421 ggttgcaggt gcggtggttg gatataccgc gggaccatcg attgcgcatt cctggggatt
      481 tagaagatcc ggctcgaaga aaccctcgcg ggagaaatcc caagccggaa tgtctccagg
      541 ccgcgtcagc caaaatacgg cggcgacggc gggaagcccg gagcagaccg gaaccatgag
      601 gacttcggca ccagccaaac ccgcgccgtc ggccgcacag cgatgggtag ggccgccggt
      661 tcaaggcctt gaatgagttt ctgccatagg caatttgaac agcgcctcaa gtatcgcgcg
      721 cgagttaaga acggcgaata gctcgcgcgg gcctaaatag cccaacgcgt cttcgaagat
      781 ctctgcgtct tcaatctttg cgcgagcaat tgccagatgg ggatctatct gtccgcgatt
      841 gcgcggttta acggataaat tcgtcacgtg cgcgtccaac aggtcagggt tgttgcgcag
      901 ctcgtgcatc gggccggcga aagtctcgcg tgacgcgttc gccaattcat tggcgcgaat
      961 caggacctgc ggcggcgatg tctgctcggg cgtgccaaat agccgaggct ggtgatgggc
     1021 cactccggaa ccggctgttg atgacacgat tgcgattgga acggccagca acagacccag
     1081 aatcaccgga gccatccaca gcaacaatgg caacgacacc gcataggcgg tgccggccat
     1141 catcacacca aacagcgtcg gcagtgcgta tttgcgaatg gtctcgccac gcgtgacgcc
     1201 gccatcgtcg cggcgctgga cctgccaacc ggcatcgcgc ccgagcagga tttccccgac
     1261 cgcggtggat tgaaagatca tcatgacggg cgcaatcagg ccggacaaga atgtctcgat
     1321 caggatgcct gcaaacacca caagccctcc accgaagtgt cttcgattcc tggattgggt
     1381 aagcagcagc acgtaagcca ggagtttggg cacgatcagc agtcccatcg tgccgaggaa
     1441 gacccaggcg gcgaggattg gatcctgagc gggccattgc gggaacaatg aaaatccctt
     1501 cggaaaatat tccggtctga caaactgggc ctgcaacgag atcaggattc caagcaccag
     1561 aaagatcagc caaagcggcg cggtcaggta cgatccgatt cccgtgagaa gatgaagccg
     1621 cgacacccag tgcaggcccc gcgccggcag aacggccaga tgctgcaggt ttccctggca
     1681 ccaccgcctg tcgcgcgcgg cgaaatccag cagcgacggc gggcactcct cgaaacttcc
     1741 gccgagcgtg ggcgccatat gaatggccca gcccgcgcgc ctcatgaggg ccgcttccac
     1801 aaaatcgtgg ctgagaatat gcccgccaaa tggtttgcgc ccgcgcaatt ccggcaggcc
     1861 ggcatcttcc gcaaacgcgc gcacccggat gacggcgttg tgcccccaat aattaccttc
     1921 cgagccgtgc caccaggcga tcccggcggc gatcagcggg ccataaagtc ttcctgcgaa
     1981 ctgctgcaat ctcgcaaaca gcgttcttgc attgaccacg atcggcagcg tctgaataag
     2041 cgcgacgttg gcatgctgtt ccatggcggc ggcgagccgc acgatcgtgt cgcccgtcat
     2101 caggctgtcc gcgtccagga taatcatatg atcatagccg gccccgaacc gtcgaaccca
     2161 gtcttcgata ttgccggact tgcgtgccgt gttctcgggg cggtgacgat aaaataattg
     2221 gccggcgccc acttcgcggc gaagctgcag gaagcacttt tcttcttcga tccagatcga
     2281 gggatcggtg gtgtcgctga gcacgtacca gtcgaattgc gatgcgcagc cggtttctcc
     2341 gatcgactcg tggatcgccc gcagccgcgc catgattcga tggggctctt cgttgtaggt
     2401 cggcagcaac atggcattac gccggctgac gaccgggagg ggtgcgtcgg ggtcgatccc
     2461 cagcgcgtcg cgcatccgga acagcagcac cacaaatccc gccagcgcgg acatgaacga
     2521 gaatgcaacc catgcaaaca gaagcacgaa cagcaccagc accatcgctt ccagcgcggt
     2581 gatgccgccg acttgcagaa cctcatatat ttcgtaacac ccggtcaccg ttagcgcagc
     2641 ggtaccgaga aaaatgtag
//
LOCUS       DASQOP010000310         4249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1772221, whole genome shotgun sequence.
ACCESSION   DASQOP010000310 DASQOP010000000
VERSION     DASQOP010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1772221"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..689)
                     /locus_tag="VE030_12300"
                     /pseudo
     CDS             complement(1..689)
                     /locus_tag="VE030_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090936.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1165..1638
                     /locus_tag="VE030_12305"
     CDS             1165..1638
                     /locus_tag="VE030_12305"
                     /inference="COORDINATES: protein motif:HMM:NF024908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZF24235.1"
                     /translation="MAKAKKPDPKSEALSQDGVLNPNPEAVRDDLFTGNPFFDAKDLV
                     QVRYEMVRRHQVDGTAISDAAATFGVTRPTFYKAQNALQTTGLAGLLPSRRGPRSGHK
                     ISTEVVAFVADLKTASPALTTSQCVSAIETRFGIKVHRRSLERALARKKKRINPT"
     gene            1909..3987
                     /locus_tag="VE030_12310"
     CDS             1909..3987
                     /locus_tag="VE030_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HZF24236.1"
                     /translation="MPELKVTADHLKRDAYLYVRQSTLRQVAEHGESTKRQYALRDRA
                     VAAGWPIDRIHVIDRDLGKSGSNAAARDGFQQLVSEVALGKAGVVMGLEVSRLARNSA
                     DWHRLIELCALTATLIVDEDGIYDPAAFNDRLLLGLKGTMSEAELHILKARMRGGQLN
                     KAKRGELEMRPPVGLVYMAEGTLGLDPDMQVQRALRLVFETFDRTGSAVQTVRFFREQ
                     GLMFPRRLLTGSNKGELLWAPPQHSRILQVLHNPRYAGAFAYGRTRTRHRPDGGTSVV
                     KVARADWQFVMPGTHQGYIDWDCFETNQRRLADNARAFAGERLSGPAREGPALLQGRV
                     LCGLCGERMSVRYSQEHGRIAPTYLCQANATRRAGWTCQSVPGKLVDAAVAALMIELM
                     TPMTLAVTLEVQRELEARAAETDAARRLHVERMQYEAELARRRYMKVDPDNRLVADTL
                     EADWNGKLRLHAEAVEEYERRSKHQAATLSAEARRRILGLAEQLPQIWNDPRVDSRER
                     KRIVRLLIDDVTLIKSETITAYVRMCGGATRTLVLDRPLPIAQIRKFKPELVATVDKL
                     LEQHCDREIADILNHDGWQTWESKPFNLKKVAFIRYAYKLASRHERLRGRGMLTTREV
                     AAKFGVSKTTVYEWGRQGLITKCCSDHLNRGLWTLPAQQTILKGCGGRDARAARLISI
                     AAQSPEQGAV"
     gene            complement(3997..4249)
                     /locus_tag="VE030_12315"
                     /pseudo
     CDS             complement(3997..4249)
                     /locus_tag="VE030_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558328.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS21 family transposase"
BASE COUNT          837 a         1301 c         1317 g          794 t
ORIGIN      
        1 ttgaggatcg aagcgatcga ccgatagctc gctgcaccaa tgctgatccc acggcgacat
       61 gcggcttcga ggcgctgcgc tccgtagctc ttctccatcc gcagaatgcc gagacaagat
      121 cggaagccct gctcgggatg aggctttgtc cgcatgatcg cttcgcacag cgccaccgtg
      181 gatggaccga tcttctcggc ggcggccagc agacgcgccg gcgtccagaa ggcgtatcgg
      241 cgatgtgcgc tcggcatgtg ctcggggatg gtggtgtgcc ggtgcttcac gcccgagcgg
      301 gcgtggcttg cgatccggtt gccgcgatgg aacacctcga tggtcgcctc ggtggcccgc
      361 acttcgacga cctggcggat caggcgggat gggaccgagt agaagtgccc ctgaacttcg
      421 acgtggtaat ccggcgccac gcgggcgcgc cgccattcgg cgtactgata gggctcggcc
      481 ggcagcggca tcagggccgg acgatcgatc gtgtcgaaga actcgcggcg gctggcgccg
      541 agcttgcgca tcagacggac gttgagatcg tcgaccagga tgcggatcgc gacgttcaac
      601 tcggcgagcg agaagaagcg ccgattgcgt aaccgcgcca agatccatcg ctgaacgatg
      661 agaaccgccg cctcgacctt ggccttatcg aaagtcgtcg actgcacgct gtgtgggtac
      721 gatctcccgc aaggtacgca tcacgcaccc gttccatccg cggcatggtc tttcattcga
      781 cttgatctgc cgacgccgtc attggggcga agatcgtgtg gtctatgagg gcccaagcgg
      841 tcgactgtgc acgatcacca gcgcattcac cgatgtcgat cctatggatg acttccggtt
      901 agttgctgcg gagagagcgg ccttccgcac ggttgatctg ttggcgttgt gcgatgtgtt
      961 gagcaggctc gccgagagca tagagaccgg cgatgcgtaa aggtaattac gccacatatg
     1021 taaacgaaat gacgcctatg cgctgccttg taagagcaac ttatcgggtt ttatctgcaa
     1081 taaatccttc atgttgcgtt agattcgcga ttgacacgcg atatattgag gcatatttat
     1141 atttacgttt tagcagggcc gcccatggca aaggcaaaaa agccagaccc gaagagcgaa
     1201 gcgctgtcgc aggacggcgt gctcaatccc aatcccgaag ccgtgcgcga cgacttgttc
     1261 accggcaatc cattcttcga tgccaaagac ctggtccagg tgcgctacga gatggtgcgg
     1321 cgccaccagg tcgacggcac tgcaatcagc gatgctgccg cgacgttcgg cgtcacccgg
     1381 ccgaccttct acaaggccca gaacgcgctg cagacgactg gtcttgcagg tttgctgccg
     1441 agcagacgcg gtcccaggag cggccacaag atctccaccg aggtggttgc cttcgttgcc
     1501 gatctcaaaa ccgcaagccc cgctctgacg acttcgcaat gtgtcagtgc gatcgaaacg
     1561 cggttcggaa tcaaggtgca ccggcgcagc ctggaacggg cgctggcgcg caaaaaaaaa
     1621 cgaatcaatc caacctgatc gtcgcgccgt cggacaccac ggagcgctac gagacgttgc
     1681 gcgccgcggt attgcgcggc gggggcgcgg cttgtcccgg tctcggcatt ctccaccgcc
     1741 gaggtgtggc ggaatggatt cgagcactcg ggcaggagcc ccctgtcgag gtggcctgct
     1801 ccgatgatcg gctcacgccc ttaaccccgc acgacccagc accggcacca agcgacatcg
     1861 ctcgtcttgt cgccagcatc ctcgtcgcca tcgcaatgga gcccgtccat gcctgaactc
     1921 aaggtcaccg ccgaccatct caagcgagac gcctatcttt atgttcgcca atcgacgctt
     1981 cgccaggttg ccgagcacgg cgagagcacg aagcgccaat acgcgttgcg tgatcgggcg
     2041 gtggcggcgg gctggccgat cgatcgcatc catgtcatcg atcgcgatct tggtaaatcc
     2101 ggatcgaatg cagcagcacg cgatggcttc caacaactcg tgagcgaggt ggcgctcggt
     2161 aaggctgggg ttgtcatggg tctcgaagtc tcgcgtcttg ctcgcaactc ggcggactgg
     2221 catcgcctca tcgagttgtg cgcattgacg gcgacgctta ttgttgacga agatggaatc
     2281 tacgatccag ccgccttcaa cgatcgcctg ctgctcggcc tcaaaggcac aatgagcgag
     2341 gccgaactgc acatcctcaa ggcgcgcatg cgtggtggtc aactcaacaa ggccaagcgc
     2401 ggcgagctcg agatgcggcc gccggtcggc ttggtttaca tggccgaggg aacactcggc
     2461 cttgatcccg acatgcaggt gcagcgcgcc ctacgcttgg tcttcgagac attcgatcgg
     2521 accggaagtg ctgtccagac agtccgcttc ttccgggagc agggcctcat gttcccgcgt
     2581 cgactgctta ccgggtcaaa caagggcgaa ctcttgtggg cgccaccgca gcattcgcgc
     2641 atcctgcagg ttctgcacaa tccgcgctac gccggcgcat tcgcgtatgg ccgcacgcgc
     2701 acacgccacc ggcccgatgg tggcaccagt gtcgtcaagg ttgccagggc ggactggcag
     2761 ttcgtgatgc ctggcacgca tcagggctac atcgactggg actgcttcga gaccaatcag
     2821 cgtcggctcg cggataacgc ccgcgccttc gctggcgagc ggctgtcggg gccggcccgc
     2881 gagggccccg cgttactcca aggtcgcgtc ctgtgcggtc tatgtggcga acgcatgagt
     2941 gtgcgctaca gccaggagca cggtcgcatt gccccgacct atctctgcca ggcaaacgcg
     3001 acacgccgcg cgggatggac atgccaatca gttcccggca agctcgtcga tgcagcggtg
     3061 gcggccctga tgatcgaact gatgacaccg atgacgctgg cggtcacttt ggaagttcag
     3121 cgcgagctcg aggcgcgcgc cgctgagacc gatgcggcgc gccgcctaca tgtcgagcgg
     3181 atgcaatatg aagccgagct ggcgcgtcgg cgctacatga aggtcgaccc cgacaaccgc
     3241 ctcgtcgccg ataccctcga ggcggattgg aacggaaagc tccgtcttca cgcagaggcc
     3301 gtcgaggaat acgagcgccg aagcaagcac caggctgcga cacttagtgc cgaggctcgc
     3361 cgacgtattc tcggtctcgc cgaacagctg ccgcaaatct ggaatgaccc gcgcgttgat
     3421 tcccgcgagc gcaaacgcat cgtacgcctg ctcattgacg acgtaacctt gatcaaatcg
     3481 gagacgatca cagcctatgt gcgtatgtgc ggcggggcta cgcgtacctt ggtgctcgat
     3541 cgcccattgc cgatcgccca gatccgcaag ttcaaaccag aactcgtcgc gacggtcgat
     3601 aagctactcg agcagcactg cgaccgtgag attgccgaca tcctgaacca tgacgggtgg
     3661 caaacctggg aaagcaagcc cttcaacctg aagaaggtcg ccttcatccg atatgcctac
     3721 aaattggcga gccgacacga gcggctacgc ggccgtggca tgctcacgac acgcgaggtc
     3781 gctgccaagt ttggcgtctc taaaaccacc gtgtacgagt ggggccgtca ggggctaatc
     3841 acaaaatgct gtagtgacca tttgaatcgt ggcttgtgga cgctgcccgc acagcagacc
     3901 attctcaaag gctgtggagg gcgcgacgcc cgcgcggccc ggctcatctc catcgctgcg
     3961 caatcgccag aacaaggtgc agtatgaaac actcgtcttg tcgcgcggtt ttcttggccg
     4021 tgccggcacg atggctgtgt tgtaatgctc cgccatttcg gcataggcac gattgacgcc
     4081 cggctcgtag aagcaggctt tggtgattcc ggacctcaga ttgtcggaaa cgaccatcgc
     4141 cggcgcaccg ccgtaaaagc caaatgcgcg tgtgtgcgag ccgatccagt cgggcagagt
     4201 ctgcgtgaag gttgcttcgg cgtaggtcaa gctcgatgcg ccgagggcg
//
LOCUS       DASQOP010000311         2440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2379947, whole genome shotgun sequence.
ACCESSION   DASQOP010000311 DASQOP010000000
VERSION     DASQOP010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2379947"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VE030_12320"
     CDS             complement(<1..534)
                     /locus_tag="VE030_12320"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HZF24237.1"
                     /translation="MAIQRRESFVLFYVIAFVTVAVAAGAVWYYWSSKQSHLVQDAKA
                     RAIELDAGLTVVVATSAKGPTARRITLVGEAAAHKSTTLYSKIGGYLTRIAVDTGDRV
                     KNGQFIAEVETPELESEYRSAVANLENKKRVLERTRNLAQKGFFSQQALENAETDVTL
                     ELGHVQELRTRLGKKALL"
     gene            complement(548..>2440)
                     /locus_tag="VE030_12325"
     CDS             complement(548..>2440)
                     /locus_tag="VE030_12325"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZF24238.1"
                     /translation="PVSFLYGVSKYLFVPLALAVVFSLLASYILSFTLVPMLSRQLLA
                     GEHHDEHGSGWSARFNRTRESVLNGMRESYGAVLTVVMRHRPFVLVCLFLIGGVSALL
                     VRVVGTDFFPTADVGIMKLHYRAPIGTRIEETEKLALAVEEEIRKIIPPEEVSVVNDM
                     IGVPLFFNLAFVPTDNISGMDADILISLKKPHKPASHYMREIRAKLPEKFPGSTFYFQ
                     NADIVTQVLNFGLPAPIDVQIQDANFERAQIYAGRIKKAIEETPGAVDVRQQQVLNYP
                     ALRVEVDRLRAAKLGFTQRDVANNMLTSLSGSGIVFPSYFLSPNGVNYTVSVQTPIDS
                     IQSVDDVLATPITSSTSGITDNSLPHTPTTVGGAPVTRLSNLAVIKPETSYQSVSHYT
                     VQRVIDIAANIDGRDLGGVVSDIRKKIDAITAEKDFPPSAKIVVRGQYEVMNASFQSL
                     ILGLILAIILVYALLVVLFQSWMDPFIIMMAVPGALIGILWMLALAGTTINVMSLMGA
                     IMAVGIGVSNSILVVSFANEVRAAHPDMTVIEAAIEAGKTRLRPVLMTALAMIIGMLP
                     MALGMGEAGEQNAPLGRAVIGGLALATVATLFVVPVVYTFLRRKMPILHELDARFEAE
                     ARGETA"
BASE COUNT          466 a          767 c          736 g          471 t
ORIGIN      
        1 cagaagggct ttttttccca gcctggtgcg cagttcctgg acgtgaccga gttcgagcgt
       61 tacatccgtc tctgcatttt cgagcgcctg ctgggaaaaa aagcccttct gcgcaagatt
      121 acgggtgcgt tccaggaccc gcttcttgtt ctcgagattc gccactgcgc tgcggtactc
      181 ggactcgagt tccggtgtct ccacctcggc aatgaactgg ccgtttttaa cgcggtcgcc
      241 ggtatcgacc gcgatcctgg tgagatagcc cccgatcttc gaatacagtg tcgtcgattt
      301 gtgtgccgcc gcctcaccca ccagcgtgat cctgcgcgca gtcggcccct ttgccgaagt
      361 cgccaccacg accgtcagcc cggcgtcgag ctcgatcgcc cgcgccttcg catcctggac
      421 cagatggctc tgcttggagc tccagtaata ccacacggca ccggcggcga cggcgacagt
      481 gacaaacgcg atgacgtaga agagaacaaa cgattcgcga cgctggattg ccatttcttc
      541 gattcctcta ggcggtctcg ccgcgcgctt cggcttcgaa tcgcgcgtcg agttcgtgca
      601 gtatcggcat tttccgtctc aggaaggtat agaccacggg cacgacgaac agcgtggcca
      661 cggttgccag cgccaggcca ccgatcaccg cgcggccaag cggcgcgttc tgctcgccgg
      721 cctcgcccat gccaagcgcc atcggcagca tgccgatgat catcgccagc gcggtcatca
      781 gcaccggacg caagcgggtc ttgccggcct cgatcgcggc ctcgattacc gtcatgtccg
      841 gatgtgccgc ccgcacttcg ttggcgaaac tcaccaccag gatcgagttc gacacgccga
      901 ttcccaccgc catgatcgcc cccatcagcg acatcacgtt gatggtggta ccggccagcg
      961 ccagcatcca caggatgccg atcagcgccc cgggcaccgc catcattatg atgaaggggt
     1021 ccatccacga ctggaacagg accaccagca gcgcatacac caggatgatc gccaggatca
     1081 gacccaggat caggctctgg aaggaggcat tcatgacctc gtactggccg cggaccacga
     1141 tctttgcgga gggcggaaag tccttttcgg cggttatggc gtcgatcttc ttcctgatgt
     1201 ccgagaccac tccgcccagg tcgcggccgt cgatattggc ggcgatgtcg atcacccgct
     1261 gcaccgtgta atggcttacc gactggtagc tggtctcggg tttgatcacc gcaaggttcg
     1321 acagccgggt gaccggcgcg ccgcccacgg tggtcggcgt gtgcggcagg ctgttgtcag
     1381 taatgcccga ggtcgaagag gtgattggcg ttgccaatac gtcgtccacg ctctggatac
     1441 tgtcgatcgg tgtctgtacc gagacggtgt agttcacccc gttcgggctg aggaagtagg
     1501 aaggaaacac gattcccgaa cccgacagcg acgtgagcat gttgttagcc acgtcgcgct
     1561 gggtgaagcc gagtttcgcc gcgcgcaacc ggtcgacctc gacccgcagc gcgggatagt
     1621 tgagcacctg ctgctggcgc acgtccaccg cgcccggagt ttcctcgata gccttcttga
     1681 tccggcccgc atagatctgt gcccgctcga agttggcgtc ctggatctgc acgtcgatcg
     1741 gcgccggcag gccgaagttc agcacctgcg tgacgatgtc ggcattctgg aagtaaaaag
     1801 tcgaaccggg gaacttctcc ggcagcttgg cgcggatctc gcgcatgtaa tgcgaagcag
     1861 gcttgtgcgg ctttttcagg gaaatgagga tgtccgcgtc catgccgctg atattgtcgg
     1921 tcggcacgaa cgcgaggttg aagaaaagcg gcacgccgat catgtcattg acgaccgaga
     1981 cttcctcggg cgggatgatc ttgcggattt cctcttcgac ggcgagcgca agcttctcgg
     2041 tctcctcgat gcgggtgccg atcggtgcgc ggtagtgcag cttcatgatg ccgacgtcgg
     2101 cggtggggaa aaagtcggtt ccgaccaccc gaaccagcaa tgcgctcacg ccgccgatca
     2161 ggaacaggca taccagcacg aacgggcggt ggcgcatcac cacggtcagt actgcaccgt
     2221 agctctcgcg catcccgtta agcacgcttt cgcgggtgcg gttgaaacgc gcgctccagc
     2281 cgctgccgtg ctcatcgtga tgttccccgg ccagcaactg ccgcgacagc atcggcacca
     2341 gagtgaagga gaggatataa gaggccagca gcgagaacac caccgccagc gccagcggta
     2401 cgaacagata tttcgacacg ccgtaaagaa acgataccgg
//
LOCUS       DASQOP010000312         2167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2380550, whole genome shotgun sequence.
ACCESSION   DASQOP010000312 DASQOP010000000
VERSION     DASQOP010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2380550"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..1071
                     /locus_tag="VE030_12330"
     CDS             37..1071
                     /locus_tag="VE030_12330"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HZF24239.1"
                     /translation="MNDLKSWQEEKSSAHLYRVVAEQEAGTARRGLFLELAREAEKQA
                     EIWEKKIREGGGVVPQRFSPDLRTRIVAALVKKMGPRPLRSVLAAMKVRGMSLYAGAD
                     PRHYVPDSTAGMERRHRGLASGGNLRAAVFGVNDGLVSNASLIFGVAGATSDSRMILL
                     SGIAGLLAGAFSMAAGEYVSVRSQREMYEYQIGLERDELGQYPEEEASELALIYAARG
                     IPEDEARRMANVILVDPDRALDTLAREELGLNPDELGSPWGAAGFSFFSFAAGALLPL
                     LPFLWSSGAGSLAVSVGLTAASLFSVGAVLSLFTGRQALRGGLRMLTIGGGAGLLTFF
                     IGKLLGVSLG"
     gene            1383..>2167
                     /locus_tag="VE030_12335"
     CDS             1383..>2167
                     /locus_tag="VE030_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HZF24240.1"
                     /translation="MSNQRIHLLHSLLKERILVLDGAMGTMIQGHKLDAHHYHGERFQ
                     AHHCDQKGNNDLLTLTQPQIIRGIHAAYLEAGADLIETNTFNSNAVSMADYGMIHLAY
                     ELNSAGARLARSAADEFTGKNPAKPRFVVGVLGPTSRTASISPDVNDPGFRNVSFDEL
                     VACYGECVKGLLDGGVDLLMVETVFDTLNCKAALFAIDDYFVRNGVRVPVMVSGTITD
                     ASGRTLSGQTTEAFWNSISHARPFSVGLNCALGAKDLRPYIEE"
BASE COUNT          415 a          631 c          699 g          422 t
ORIGIN      
        1 ctctgtgcct ctgtgtctct gtggtgagat cagtcgatga atgatctgaa aagctggcag
       61 gaagagaaga gttccgcaca cctgtaccgt gtggtcgcgg agcaggaggc cggcacggcg
      121 cgccgggggc tgttccttga actggcacgc gaagcggaga aacaggccga gatctgggag
      181 aagaagatcc gcgaaggcgg cggcgtggtt ccgcagcgct ttagtccgga cctgcgtacc
      241 cgcattgtcg ccgcactggt caaaaaaatg gggccacgcc ccttgcgttc ggtgctggcc
      301 gcgatgaagg tgcgcggcat gtcgctgtat gccggcgccg atccgcgcca ctacgttccg
      361 gattcaaccg cgggaatgga gcgccgccat cgggggctgg cgagcggcgg gaatttgcgt
      421 gccgcagtct tcggggtaaa cgacgggctg gtctcgaacg cgagcctgat tttcggcgtc
      481 gcgggtgcaa cttccgatag caggatgatt cttctgtccg gaattgccgg actgcttgcc
      541 ggcgcattct cgatggcggc aggcgagtac gtgtcggtgc gctcgcagcg cgaaatgtac
      601 gagtaccaga tcggcctgga gcgcgacgaa ctcggccagt acccggagga agaagcctcc
      661 gaattggcgc tgatttacgc cgcccgcggc attcccgagg acgaggccag gcggatggcc
      721 aacgtgattc tggtggaccc agacagggca ctggacacgc tcgcgcggga agagcttggc
      781 ctcaatccgg acgagctggg ttcgccctgg ggcgcggcgg gattctcatt tttctcgttt
      841 gcggccggcg ccctgttgcc gttgctgcct ttcctgtgga gctccggggc ggggagtctg
      901 gcagtctcgg tcgggcttac cgcggcgtcg ctgttctcgg tcggtgcggt gctgtcgcta
      961 ttcaccggcc gacaggcgct acgcggcgga ctgcgcatgc tcacgatcgg cggtggggcc
     1021 ggactgctga cgtttttcat agggaaactt ctcggggtga gcctgggatg aggagcaagg
     1081 atgaaggatg aaggatgaag gcggaggggc aaggatgaag gcggaaggat gaaggatgaa
     1141 ggaaaatcaa aaacaaacta aagattcctc gtgatctcgc accggccaat cgcttctcac
     1201 ttcatccttc atccttcatc cctcatcctt gctcttcccg attgacacca acccatcaac
     1261 ccgctaacgt tgttgttgcg ccgatttgat actcagcttg cgggcgctta acaaataccg
     1321 ctaaagcgtg gaacgaaaac ccgtcccgct caagcggacg ggtttttgtt ttcggagtcg
     1381 gaatgtcaaa ccagcgcata catctgctgc acagcctgct caaggaacgc atcctggtcc
     1441 tggacggcgc aatgggcacc atgatccagg gccacaagct ggacgcccac cactatcacg
     1501 gcgagcgctt ccaggcgcac cactgcgacc agaagggcaa taacgatctg ctgaccctga
     1561 cccagccgca gatcattcgc ggaatccatg cggcctacct ggaagcgggt gccgacctga
     1621 tcgagaccaa cacgttcaac tcgaatgcgg tgtcgatggc cgactacggc atgatccatc
     1681 tggcctacga attgaacagc gcaggggcgc ggctggcgcg gtccgctgcc gacgagttca
     1741 ccgggaaaaa tcccgccaaa ccgcgcttcg tcgtcggagt gcttgggccc accagccgca
     1801 ccgcatcgat ttcgccggac gtgaacgatc cgggatttcg caacgtttcc ttcgacgaac
     1861 tggtggcctg ttacggcgaa tgcgtgaaag gcttgcttga cggtggcgtg gatctgctga
     1921 tggtcgagac ggtattcgac acgctcaact gcaaggccgc gctgttcgcg atcgacgatt
     1981 acttcgtccg taatggcgtg cgcgtaccgg tcatggtgtc cggcaccatt accgatgcca
     2041 gcggccgcac gctttcgggg cagaccaccg aagcgttctg gaactcgatt tcccacgcgc
     2101 ggccgttctc ggtcggactg aattgcgcgc tcggtgccaa ggacctgcgg ccctacatcg
     2161 aggagtt
//
LOCUS       DASQOP010000313         5870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_968350, whole genome shotgun sequence.
ACCESSION   DASQOP010000313 DASQOP010000000
VERSION     DASQOP010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5870
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968350"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /gene="nudC"
                     /locus_tag="VE030_12340"
     CDS             <1..765
                     /gene="nudC"
                     /locus_tag="VE030_12340"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HZF24241.1"
                     /translation="FHGSQILIVRGAQSASVPSASDPREFGLAPQRSQYLGILGEQHC
                     FACELEQNAALPAGMQLSGLRALFGALDDSLFALAGRAFQIVDWNRSHQFCGHCGTPT
                     ELKMNERARVCPNCGQSHYPRIAPAVMALVRRGSELLLARSPHFPPGMYSALAGFVEP
                     GETLEQTLVREVREEVGIEVANLRYFASQPWPFPHSLMIAFYADYAGGEIAPDPSEIE
                     AAGWFSADRLPQALPSKISISRRLIDAALAELGASS"
     gene            794..1414
                     /locus_tag="VE030_12345"
     CDS             794..1414
                     /locus_tag="VE030_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HZF24242.1"
                     /translation="MLSIWGRSNSVNVQKPLWCCEEMGVQYERVDAGGTFGVVNTPQY
                     RNLNPNGLVPTIEDDGFVLWESNAIVRYLAAKHSAGKLWPEDLKVRAEADKWMDWQSS
                     TFWPTFRPLFWNLVRTPADQRDEGAMEDSRLKTAEILEYLDGHLKNRAYVAGDALTMG
                     DIPMGCAIQRWMTLPIDHPEMPNLERWYDALAGRPAYKKVVMLPLT"
     gene            complement(1474..2643)
                     /locus_tag="VE030_12350"
     CDS             complement(1474..2643)
                     /locus_tag="VE030_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HZF24243.1"
                     /translation="MRAKPLSGIRVLDLTRLLPGPVCTLHLADLGADVIKIEDTGAGD
                     YARTMGAGAGRTAPLFLAINRNKRALRLDLKKPEGVEVFLRLARDADIVVESFRPGVV
                     DRLGIGYSACSEVNPRIVYCAISGYGQTGPYRDLAGHDVNYCGYAGFIDQVGPAGGDP
                     VIPNLQVADILGGAVVPAMGILAALVDAQRSGQGRYVDVAMTEGVLSHNLQALAAVAS
                     AGHVHPRGEDFLSGREPCYAVYRSSDGKYMAVGALERKFWDRVCDVLARPDLKACHWE
                     VRDRDPQWGKEELSRIFAAWPQVHWVEKFRGKDCCVTPVLALEESLEDPQIRARGMVV
                     RDGEVLQLAPPLKMSEYEFAVERHAPAPGEHSDQILREAGYADAAIGKLRVAGVI"
     gene            complement(2643..3206)
                     /locus_tag="VE030_12355"
     CDS             complement(2643..3206)
                     /locus_tag="VE030_12355"
                     /inference="COORDINATES: protein motif:HMM:NF022586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HZF24244.1"
                     /translation="MTLSTPASRKLLHNRKVECFGYQRDDGLWDIEGHLVDTKTYDFA
                     NRDRGGIKAGEPLHEMWLRLTLDLDLKVHDVEARTVWGPYSICGDITENFKALIGLSI
                     KPGWSQKTRELLGGTKGCTHLVELLGPIATTGFQTVYAARAERSKSAGKRERPALIDS
                     CHAWASDSPVVAKRFPEFYTGPKKTPG"
     gene            complement(3203..3400)
                     /locus_tag="VE030_12360"
     CDS             complement(3203..3400)
                     /locus_tag="VE030_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015422134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HZF24245.1"
                     /translation="MNPERNDIRSPCVDVCQMNPFTGLCRGCYRTLDEIAGWLDFSAS
                     EKLEVLKRLDERRPGHALRLK"
     gene            complement(3397..4314)
                     /locus_tag="VE030_12365"
     CDS             complement(3397..4314)
                     /locus_tag="VE030_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HZF24246.1"
                     /translation="MKYPKRVRMVEVGPRDGLQNEPGGVPAEVKVALIDHLSDAGFQV
                     VEAGSFVSPKWVPQMADTAEVMAQIRRKPGVTYPVLVPNMKGLEAAIAAKATEVAGFA
                     AATEAFSKRNTNCTIAESVQRQAEVCKAALGAGLRVRGYISVVLGCPYEGEVKPEAVA
                     AVSQRLYDMGCYEISLGDTIGTGTPAKAQRMIEAVAKKIPVEKLAGHFHDTYGQALAN
                     ILAVMEMGVATFDSSVAGLGGCPYAKGATGNVASEDVLYMLDGLGIETGVDMAKLLDA
                     AEFICAQLKRPTVSRAGRALSAKREKAAA"
     gene            complement(4311..>5870)
                     /locus_tag="VE030_12370"
     CDS             complement(4311..>5870)
                     /locus_tag="VE030_12370"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HZF24247.1"
                     /translation="YPVLIKASAGGGGKGMRVVGKAADFADALAAAKREAASSFGDDR
                     VLIEKYLTRPRHIEVQVFADTQGHCVYLFERDCSVQRRHQKILEEAPAPGMTAERRRE
                     MGNAAVSAARSVGYVNAGTVEFIVDEHGHFYFMEVNTRLQVEHPVTEMITGLDLVEWQ
                     LRVASGEPLPLAQEQLAINGHAIEARVYAEDPVRGFLPAAGRIEHLRQPEESAHVRVD
                     TGVQAGDEVGVHYDPMIAKLIAWDSDRPSALRRLHAALGEFQIVGLTSNLAFLSAVTA
                     HRAFADAHREPGLLDTGLIERYRTELSPDAGPASDPILAIAILSELLRMDREAAQSAK
                     RSADPYSPWNARDGWRLNEDNHHTFVFIDAGKRVEVVAHYRNRGYLLELPGGKMLASG
                     EVAGDRTIDADLSGSRTRATVVRRANELTVFAFGSSHSLEFETPATVEEEDPSGRLVS
                     PLPGAVIQVLIEDGEAVQKGQALMIIEAMKMEHTISAPQSGTVKRIYFAAGEQVAEGA
                     QLLEFEDTTKA"
BASE COUNT         1009 a         1952 c         1839 g         1070 t
ORIGIN      
        1 ttccacggtt cgcagatcct gatcgtgcgt ggcgcgcaaa gcgcgagcgt cccgtcggcc
       61 tccgatccac gcgaattcgg actcgcgccg caacgcagcc agtacctcgg catcctcggg
      121 gagcagcatt gcttcgcctg cgaactggag cagaacgctg cgctgccggc gggcatgcag
      181 ttgtcgggat tgcgtgcgct tttcggtgcg ctcgacgatt cgctgttcgc gctggcgggg
      241 cgcgcattcc agatcgtcga ctggaaccgg tcccaccagt tctgcggcca ctgcggcacg
      301 ccgaccgagc tgaaaatgaa cgagcgggcg cgcgtttgtc cgaattgcgg ccagtcgcat
      361 tacccgcgca tcgcccccgc ggtgatggcg ctggtcagaa ggggaagcga gttgttgctg
      421 gcgcgctcgc cgcatttccc gcccgggatg tacagcgcgc tcgccggctt cgtcgagccg
      481 ggagaaaccc tggagcagac gctggtgcgc gaagtgcgcg aggaagtcgg catcgaagtc
      541 gccaacttgc gatacttcgc cagccagccg tggccgttcc cgcattcact gatgatcgcg
      601 ttctacgctg actacgccgg tggcgagata gcccccgatc cgagcgaaat cgaagccgcg
      661 ggctggttta gcgcagaccg gctgccgcag gcattgccga gcaagatcag catttcccga
      721 cgactgatcg atgcggcgct cgccgaactc ggcgcttcaa gctgacaaca agatcccaac
      781 cggggaggat gccatgctga gtatctgggg ccgcagcaac tcggtcaacg tccagaagcc
      841 gctgtggtgc tgcgaggaaa tgggcgttca gtacgagcgg gtggacgccg gcggcacctt
      901 cggcgtggtg aacacgccgc aataccgcaa cctcaatccg aacggcctgg tgccgaccat
      961 cgaagacgac ggcttcgtgc tctgggagtc gaacgcgatc gtgcgctatc tcgccgccaa
     1021 gcacagcgcg ggcaaactct ggcccgagga cctcaaggtg cgcgccgaag ccgacaagtg
     1081 gatggactgg cagagttcga cgttctggcc gaccttccgg ccgctgttct ggaacctggt
     1141 gcgcactccg gccgatcagc gcgacgaagg cgcgatggaa gactcacgcc tcaagaccgc
     1201 cgagatcctg gagtatctcg atgggcacct caagaaccgc gcgtacgtcg ccggcgacgc
     1261 gctcaccatg ggcgacattc cgatgggttg tgcgatccag cgctggatga ccctgcccat
     1321 cgaccatccg gagatgccga atctggagcg ctggtacgac gcgcttgcgg gccgcccggc
     1381 gtacaagaag gtggtgatgc tgccgctgac ctgaactgag ccggccagtc accctgttgc
     1441 ggagttctgg atgagtgagc cggcagcgcg gcttcagatc acgccggcca cgcgcaattt
     1501 cccgatcgcc gcatcggcat agccggcttc gcggagtatc tggtcgctgt gttccccggg
     1561 cgctggtgca tggcgctcca ccgcgaactc gtattcgctc attttcagag gcggggccag
     1621 ttgcagcact tcgccgtcgc gcacgaccat ccctcgcgcc ctgatttgcg gatcttcgag
     1681 gctctcctcg agcgcgagca ccggggtcac gcagcagtct ttaccgcgga atttttctac
     1741 ccagtggact tgcggccagg cggcgaagat tctgctcagt tcttcctttc cccactgcgg
     1801 atccctgtcg cggacttccc agtggcaggc cttgaggtcg ggccgcgcca gcacatcgca
     1861 gacacggtcc cagaatttcc tctccagcgc gccgaccgcc atatacttgc cgtcggagct
     1921 gcgatagacc gcgtagcagg gttcgcggcc ggagagaaag tcctcgccgc ggggatgaac
     1981 atgtccggcg gaagccacgg cagcgagcgc ctggaggttg tgcgaaagca cgccctcggt
     2041 catggctacg tcgacgtacc gtccctgacc gctgcgctga gcgtccacca aagccgcaag
     2101 aatacccatg gccggcacca ccgcgccgcc gaggatgtcc gcgacctgga gattcggtat
     2161 caccggatcg ccgccggccg gtccgacctg atcgatgaac ccggcgtagc cgcagtaatt
     2221 cacgtcgtgc ccggccaggt cgcggtaagg accggtctgt ccgtacccgg aaatcgcgca
     2281 atagacgatt cgcggattga cttcactgca cgcggaatat ccaatgccga gccggtctac
     2341 gacccccggg cggaaactct ccaccacgat gtccgcgtcg cgcgccaggc gcaggaacac
     2401 ttcgacgcct tccggttttt tcaagtcgag gcgcagcgcg cgcttgttgc ggttgatggc
     2461 gagaaacagg ggtgccgtcc gaccggcccc ggcgcccatc gtgcgggcat agtcgccggc
     2521 gccggtgtct tcgatcttga tgacatcggc gccgagatcg gccagatgca gcgtgcacac
     2581 cgggccggga agaagacggg tgagatcgag aacgcgaatc cccgagagcg gtttggcgcg
     2641 catcagcctg gggttttctt cgggccggta tagaactccg gaaagcgttt tgcaaccacc
     2701 gggctgtcgc tcgcccaggc gtgacaggaa tcgatcagcg ccggccgttc gcgtttgccc
     2761 gcggatttgc tccgttccgc ccgtgccgca tacaccgtct ggaaaccggt ggtcgcaatc
     2821 gggccaagca actccacaag atgggtgcag cccttggtgc cgcccagcag ctcgcgcgtt
     2881 ttctggctcc agcccggctt tatggacagc ccgatcagcg ccttgaagtt ctcggtgatg
     2941 tcgccgcaga tactgtaggg tccccacacc gtgcgcgcct cgacatcgtg caccttgagg
     3001 tcgagatcga gggtcagccg cagccacatc tcgtgcaacg gctcgcccgc cttgatgcca
     3061 ccgcggtcgc gattcgcgaa atcgtaggtc ttggtatcca cgaggtgccc ctcgatgtcc
     3121 cacaggccgt cgtcgcgctg gtagccgaaa cattcgacct tgcggttgtg caggagcttt
     3181 cgggaagcag gggtgctgag cgtcatttca gtcggagggc gtggcccggg cgtcgctcgt
     3241 cgaggcgctt gagcacttca agtttctcgg acgcggaaaa atcgagccag ccggcgattt
     3301 cgtcgagagt gcgatagcag ccgcggcaca gcccggtgaa cggattcatc tggcagacat
     3361 cgacgcaggg cgaccggatg tcgttgcgct ccggattcat gccgccgctt tctcgcgttt
     3421 ggcggatagc gcgcggccgg cgcgcgaaac cgtcggccgc ttgagttgcg cgcagatgaa
     3481 ctcggcggca tcgagcagct tggccatgtc cacgccggtt tcgataccca ggccgtcgag
     3541 catgtatagc acgtcttcgc tggcaacatt gccggtcgcg cccttcgcat acgggcagcc
     3601 gcccagtccc gccaccgaac tatcgaacgt ggccaccccc atttccatca ccgcgaggat
     3661 gttggcgagc gcctgtccgt aggtgtcgtg gaaatgaccg gcgagcttct cgactggaat
     3721 ctttttcgcc accgcttcga tcatgcgttg cgccttggcc ggagtgccgg tgccgatggt
     3781 gtcgccgagc gagatctcgt agcaacccat gtcgtagagg cgttgcgaca ctgccgcaac
     3841 ggcttccggc ttgacctcgc cttcgtaggg acagccgagt acgaccgaga tgtaaccacg
     3901 cacccgcagg ccggcaccga gcgccgcctt gcatacctcg gcctggcgtt gtaccgactc
     3961 ggcaatcgtg cagttggtat tgcgttttga aaaggcctcg gtggcggccg cgaagcccgc
     4021 cacttccgtc gccttcgctg cgatcgcggc ctccaacccc ttcatgttcg gcaccagcac
     4081 cggataggtc acgcccggct tacgccggat ctgcgccatc acctccgcgg tatcggccat
     4141 ttgcggcacc catttcgggg agacgaaact cccggcttcg acaacctgaa atcccgcgtc
     4201 ggacaaatga tcgatcagcg cgaccttgac ttcggcaggg acgccgcccg gttcgttctg
     4261 caagccgtcg cgcggaccga cttcgaccat cctgacgcgt ttcggatatt tcatgctttg
     4321 gtcgtatcct cgaattcgag caactgcgct ccttcggcaa cctgctcgcc tgccgcaaag
     4381 tatatccgct tgaccgtccc gctctgcggc gccgagatgg tgtgctccat tttcatcgcc
     4441 tcgatgatca taagcgcctg gcctttctgt accgcttcgc cgtcctcgat cagcacctgg
     4501 atcacggccc cgggcagcgg cgagaccagc ctgccggacg gatcctcttc ctcgacggtg
     4561 gcgggggtct cgaattcgag actatggctg ctgccgaagg caaacaccgt cagctcgttg
     4621 gcccggcgga ctaccgtggc gcgggtacga ctgccggaga gatcggcatc gatcgtccgg
     4681 tcgccggcga cttctccgga ggccagcatc tttccaccgg gcagttccag cagataaccc
     4741 cggtttcggt agtgcgccac cacttccacg cgcttgccgg cgtcgatgaa cacgaaggtg
     4801 tgatggttgt cctcgttcag gcgccagcca tcgcgcgcgt tccagggcga gtaagggtcc
     4861 gccgagcgct ttgcggactg cgccgcttcg cgatccattc gcagcagttc gctcaatatc
     4921 gcgatcgcca ggatcgggtc ggacgccggc ccggcgtccg gggacagctc ggtccgatag
     4981 cgctcgatca ggccggtatc gagcaatccc ggctcgcgat gcgcgtcggc aaatgcgcga
     5041 tgcgcggtga cggcggaaag aaacgccaga ttgctggtca gcccgacgat ctgaaactcg
     5101 ccgagcgcgg cgtgcaagcg gcgcaacgcg gaagggcggt ccgaatccca ggcaatcagc
     5161 ttcgcgatca tcggatcgta gtgcacgccg acttcgtcgc ccgcctgcac gccggtgtcc
     5221 acgcgcacgt gcgccgactc ctccggctgg cgcaggtgtt cgatgcgccc ggcggcggga
     5281 agaaaaccac gcacggggtc ttccgcgtac acgcgcgcct cgatcgcgtg gccgttgatc
     5341 gccaactgct cctgcgccaa cggcagcggt tccccggaag caacccgcaa ctgccattcg
     5401 accagatcga gcccggtgat catctcggtc accggatgct cgacctgcag ccgggtattc
     5461 acttccatga agtagaagtg tccgtgctcg tcgacgatga attccaccgt gccggcattc
     5521 acgtagccga ccgaccgcgc cgccgaaacc gcggcgttgc ccatttcgcg tcggcgttca
     5581 gccgtcattc ccggtgccgg cgcctcctcg aggatcttct gatgacgacg ctgcaccgag
     5641 cagtcgcgct cgaacaagta cacgcaatgc ccctgcgtgt ctgcgaacac ctgcacctcg
     5701 atgtgccgtg gacgggtcag gtacttctcg atcagcaccc ggtcatcgcc gaacgaagag
     5761 gccgcttcgc gtttcgcagc ggcgagggca tcggcaaaat ccgccgcctt cccgaccacg
     5821 cgcatgccct tgccgccgcc gcccgcggac gccttgatca gcaccgggta
//
LOCUS       DASQOP010000314         5773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_564701, whole genome shotgun sequence.
ACCESSION   DASQOP010000314 DASQOP010000000
VERSION     DASQOP010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5773
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564701"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VE030_12375"
     CDS             <1..244
                     /locus_tag="VE030_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZF24248.1"
                     /translation="VLERLAREAREHGALVIMVVHDLLFASRYCDRFLLLYGDGRFAH
                     GGAQEVLDAGHLGELYGFPLDVVEAGLDRLFLPRRA"
     gene            308..1666
                     /gene="pcnB"
                     /locus_tag="VE030_12380"
     CDS             308..1666
                     /gene="pcnB"
                     /locus_tag="VE030_12380"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HZF24249.1"
                     /translation="MIRKLLRRVFSGRSSDSTEPQVIPRSKHGVSRDGISTSARRVIS
                     TLQEKGFAAYVVGGAVRDLLLGLKPKDFDVATSATPDQVRSAFRRSRIIGRRFRLVHV
                     MFGNDTVEVSTFRARQGNDPAEQVSDEHGRILRDNVFGTQEEDATRRDFTINALFYDP
                     TSEEIHDFCGGLDDLKTRRLRMIGDAEQRYREDPVRMLRAVRLAAKLGLKIEAKTREP
                     IKRLAPLLANVPSSRLFEEMMKLLFSGHATACLLQLRQEGLHHGLLPMLDVILEQPMG
                     ERFVMMALKSTDERVRADKPIAPGFLFAALLWHEVLAAWKLAQEEGLNPMPALFKAMD
                     HVIQVQAEKLSIPRRFGTDMKDIWALQPRFLGRSGRRPYSVLAHPRFRAGYDFLVLRC
                     DSGEVDAEVAEWWENFQNADEDTRKQMLLPDRTQRRRRRRRKPGGGNPPDSNPVPVPA
                     ES"
     gene            1666..2172
                     /gene="folK"
                     /locus_tag="VE030_12385"
     CDS             1666..2172
                     /gene="folK"
                     /locus_tag="VE030_12385"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HZF24250.1"
                     /translation="MPGNEPVAAIAFIGLGSNLADPLVQVRQALTDLESIPGTRVTAR
                     SSLYRTSPVGYLEQPDFINAVASVQTTLKPQALLAALLAIENRRGRRRAMRNAPRTLD
                     LDLLLYGEEVLEQHGLTLPHPRLHERAFVLAPLAEIAPEAMVPGRGRVQDLLARIDRK
                     GVSRIDAA"
     gene            2162..2803
                     /locus_tag="VE030_12390"
     CDS             2162..2803
                     /locus_tag="VE030_12390"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HZF24251.1"
                     /translation="MLPEKYRYIVVEGPIGAGKTSLARRLSEHVGKSLLLEDPDSNPF
                     LARFYEDQRRYALATQLFFLFQRAQQVAGLKQIDLFDRGTVADFIFDKDPLFASLTLS
                     DDELHLYQQIYGHLTPQAPTPDLVIYLQASPAKLFARVRKRAVAYERNISEQYLSRLA
                     DAYRRFFYQFSAAPLLIVNSDHLNFVDDANDFDLLLRRISEMRGPREFFSRGE"
     gene            2969..3760
                     /gene="panB"
                     /locus_tag="VE030_12395"
     CDS             2969..3760
                     /gene="panB"
                     /locus_tag="VE030_12395"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HZF24252.1"
                     /translation="MKVTTSVLQKMRQEGERITMLTCTDASFAALLDTAGVDTFLVGD
                     SLGMTIQGHDSTLPVTMDDMVYHTRIVARGANRALVIGDMPFGSYQQSPQKAFENAAR
                     LMSAGAQMVKLEGGEVMAETVAFLSARGIPVCGHVGLTPQSVHAIGGYKVQGKTDDSA
                     QQLIHAGKALQDAGASLMVVELVPASVGKALTEALAIPVMGIGAGPHCSGQILNLYDM
                     LDIYPGKKPRFVKNFMDGSASIRAAIERFVAEVKAGTFPGSEHSF"
     gene            3850..4680
                     /gene="panC"
                     /locus_tag="VE030_12400"
     CDS             3850..4680
                     /gene="panC"
                     /locus_tag="VE030_12400"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HZF24253.1"
                     /translation="MEVIKTTKDLRERLKRESRVACVPTMGNIHDGHLSLVRIAREHA
                     PVTVTTIFVNRLQFGQGDDFEKYPRTLEEDCAKLEAGGNTIVFAPDEKQMYPEPQVFY
                     IDLPKVASKLEGRFRPGHFRGMATVVLKLFNSVQPQIAVFGKKDYQQLAIVRHMVEQF
                     ALPITIIPAETVRAEDGLALSSRNRFLSPAERKEAIRLRQAILQVKDAVERGDRNFIA
                     MEYAAGELLARHDWKVDYVVVRTRRGLRPEAQDKKLVVLGAAKLGTTRLIDNQEILAP
                     "
     gene            4902..>5773
                     /locus_tag="VE030_12405"
     CDS             4902..>5773
                     /locus_tag="VE030_12405"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase A"
                     /protein_id="HZF24254.1"
                     /translation="MTILYYSEADPAEPWREVFARELPRFAFRVWPDSGPLESVRYAL
                     VWKPPRGFLAQFPNLKAILALGAGVDPIPTDPALPKDVPLLRLVDVGFPAQMAEYAMY
                     AVLHFQRRMGDIEALQRQARWNQLDPFFVRDFPVGVMGLGVIGAVVAQRFAAAGYAVA
                     GWTRGPRQVDGIDVFAGSETFRKFLARSRVVVNALPLTPQTESVLDAAAFAAMPKGGY
                     VVNIGRGAHVVDRDLIAALDGGQLAGAMLDVFREEPLPSSHPFWRHPKIVVTPHVAAP
                     TIASEVQAQVIENI"
BASE COUNT         1086 a         1754 c         1819 g         1114 t
ORIGIN      
        1 ggtgctcgag cgcctggcgc gtgaggcccg cgagcacggc gcgctggtga taatggtggt
       61 ccacgacctg ctgtttgcaa gccgctattg cgaccggttc ctgctgctgt acggcgacgg
      121 gcgattcgcg cacggcggcg ctcaggaggt gctggacgcc ggccatcttg gcgagcttta
      181 cggttttccg ctcgatgtag tagaggcggg ccttgaccgg ttgttcctgc cgcgccgggc
      241 ttgagggtac atgccgcatg tataatcgcc gcttttacgc agtaccaact ccttcccgcg
      301 atacttgatg attcggaaac tgcttcgccg ggtgttctcc ggccggtcat cagactccac
      361 agagccgcag gtcattccgc gcagcaaaca cggcgtttcc cgcgacggta tcagtaccag
      421 tgcccgtcgg gtcatcagca ccctgcagga aaagggcttc gccgcctacg tcgtcggcgg
      481 cgccgtacgc gacctgctgc tcggactcaa gcccaaggat ttcgacgtcg ccaccagcgc
      541 cacgcccgat caggtacgca gtgctttccg gcgctcgcgc atcatcggcc ggcgtttccg
      601 gttggtgcac gtcatgttcg gcaacgacac ggtggaggtt tccacgttcc gggccagaca
      661 gggcaacgac ccggccgaac aggtgtcgga cgaacacggg cgcatcctgc gcgacaacgt
      721 attcggcacc caggaagaag atgccacgcg ccgcgacttc accatcaacg cgctctttta
      781 cgatccgacc agcgaagaga ttcacgactt ctgcggcggc ctggacgacc tcaagacccg
      841 gcggctgcgg atgatcggcg atgcggaaca gcgctatcga gaggatccgg tgcgcatgct
      901 gcgtgcggtg cggcttgccg ccaagctcgg cctgaaaatc gaggcaaaga cccgcgagcc
      961 gatcaagaga ctggcgccct tgctcgccaa tgtgccctcg tcgaggctgt tcgaggaaat
     1021 gatgaagctg ctgttctccg gccatgccac tgcctgtctg ctgcaattgc gccaggaagg
     1081 actgcatcac ggcctgctgc cgatgctcga cgtgattctc gagcagccga tgggcgagcg
     1141 cttcgtgatg atggcgctga agagcacgga cgaacgcgtg cgcgcggaca aaccgatagc
     1201 gccgggcttc ctgtttgcgg cgctgttgtg gcacgaagtg ctggcggcgt ggaagctggc
     1261 gcaggaggag ggcctgaatc cgatgccggc gctgttcaag gcgatggatc acgtgattca
     1321 agtgcaggcg gagaaactgt cgatcccgcg ccgcttcgga accgacatga aggatatctg
     1381 ggccctgcag ccgcgcttcc tcggccgttc cgggcggcga ccttacagcg tgctcgcgca
     1441 tccgcgtttt cgcgccggct atgattttct ggtgctccgc tgcgacagcg gcgaagtgga
     1501 cgccgaagtc gccgagtggt gggaaaactt ccagaatgcc gacgaagaca cgcgcaagca
     1561 gatgctgctt cccgaccgca ctcagcgccg gcgccggcgg cgtcgcaagc ccggcggcgg
     1621 caatccaccg gacagcaatc ctgttcccgt gccggcggaa tcctgatgcc cggaaatgag
     1681 ccggtggcgg cgattgcttt tatcggcctg ggcagcaatc tcgccgatcc cctggtgcag
     1741 gtaaggcagg cgctcacgga tctggaatcg atccccggta cccgggtgac cgcgcgctcc
     1801 tctttgtatc gcacgtcccc ggtcggttat ctggaacaac ccgatttcat caacgccgtc
     1861 gcgtcggtgc aaaccacgct caagccgcag gcgcttcttg ccgcgcttct tgccatcgag
     1921 aatcgccgcg ggcgcaggcg cgcgatgcgc aatgcgccgc gtacgctcga cctcgacttg
     1981 ctgctctacg gagaagaggt cctcgagcag cacggcctga cgctgccgca cccgcggctg
     2041 cacgaacgcg ctttcgtgct ggcgccgctt gccgaaatcg cccccgaggc catggtgccc
     2101 gggcgggggc gggtgcagga cctgctggcg cggatcgacc gcaaaggagt gagcagaatc
     2161 gatgctgcct gagaaatacc gatacatcgt ggtcgaggga ccgatcggcg ccggcaagac
     2221 cagcctggcg cggcggctgt cggaacatgt gggcaagagt ttgttgctcg aggatccgga
     2281 ttcgaatccc tttcttgcgc gtttctacga agaccagcgg cgctatgcgc tggcgacgca
     2341 actgtttttc ctgttccagc gggcgcaaca ggtcgcgggc ctgaagcaga tcgatctgtt
     2401 cgatcgcggc acggtcgccg atttcatctt cgacaaggac cccttgtttg ccagccttac
     2461 gctttccgat gacgaattgc atctctacca gcagatctac ggccacctga cgccacaggc
     2521 gcccacgccg gacctggtga tctacctgca ggcatcgcca gcgaaactgt tcgcgcgtgt
     2581 acgaaagcgt gcggtcgcct acgaacgcaa catctcggag caatatctca gccggctggc
     2641 cgatgcctac cgccgcttct tctaccagtt ttccgccgcg ccgctgctga tcgtcaacag
     2701 cgaccatctg aattttgttg atgacgccaa cgattttgat ttattgttgc gccgcatcag
     2761 cgagatgcgc ggaccccgcg agttcttcag ccgtggcgaa taagtcaaac taaccacaga
     2821 gacacagagg cacagagacg ggcgagaatt ctcaagactt ccatccacgg tgggggatga
     2881 ttgttcctcc gttcgaggtg tctttggttt caagtagtga ttctgttttt ctccgcgtct
     2941 cagtgtctct gtggtgaaag ggttttggat gaaagtaacg acgagcgttt tgcaaaagat
     3001 gcggcaggag ggcgagcgca tcaccatgct gacctgtacc gatgcgagtt ttgccgcact
     3061 gctggataca gcgggcgtgg atacgtttct ggtcggggat tcgctgggga tgacgatcca
     3121 gggccacgac tcgaccttgc ccgtgaccat ggacgacatg gtttatcaca cgcgcatcgt
     3181 ggcgcgcggc gccaaccgcg cgcttgtgat cggcgatatg cccttcggca gttaccagca
     3241 aagcccgcaa aaggcgttcg agaatgccgc acggttgatg agcgccggcg cacaaatggt
     3301 aaagctcgag ggcggcgaag tgatggcgga aaccgtggcg ttcctgagcg cgcgcggtat
     3361 ccccgtttgc ggccacgtcg gattgacgcc gcaatcggtg cacgcgatcg gcggctacaa
     3421 ggtgcagggc aaaaccgatg attccgcgca gcagctgatt catgccggca aggcgttgca
     3481 ggatgcggga gcaagcctga tggtggtgga actggtcccg gcatccgttg gcaaggcgtt
     3541 gaccgaagcc ctggcgatcc cggtgatggg tatcggagcg gggccgcatt gctccgggca
     3601 aatcctcaat ctctacgaca tgctcgatat ctatcccggc aagaagccac gtttcgtgaa
     3661 gaatttcatg gacggctcgg cttcgatccg ggcggcgatc gagcgcttcg tagccgaagt
     3721 gaaagccggc actttccccg gttccgaaca cagcttttag aggaggcaag ggcgagggac
     3781 gaggaacgag ggaaaggcgc ggcccggccg aatcaaaccc ctcacccctc gtccctcgcc
     3841 cctccaaaaa tggaagtcat taaaacaaca aaagatctgc gcgagcggct gaagcgtgaa
     3901 tcacgcgtcg cctgcgtgcc gaccatgggc aacattcacg atggccatct gagcctggtc
     3961 aggattgccc gcgagcacgc tccggtcacg gtcaccacga ttttcgtcaa ccgcctgcag
     4021 tttgggcagg gcgacgactt cgagaagtac ccgcgtacat tggaggaaga ctgcgccaaa
     4081 ctggaagccg gtggtaacac catcgtcttc gccccggatg aaaagcagat gtatcccgaa
     4141 ccacaggtct tttacatcga cctgcccaag gtcgccagca agctcgaggg ccgcttccgg
     4201 ccgggccatt tccgcggcat ggccaccgtg gtgttaaagc tgttcaattc cgtgcagccc
     4261 cagatcgccg tattcggcaa gaaggattat cagcaattgg caatcgtgcg gcacatggtc
     4321 gagcagtttg ctctgccgat caccatcatt ccggcggaga cggtgcgcgc cgaggacggg
     4381 ctggcgctgt cctcgcgcaa ccgcttcctg tcccccgcgg aaaggaagga agcgattcgc
     4441 ctgcgccagg ccatccttca ggtcaaggac gcagtggaac gcggcgaccg caacttcatc
     4501 gcgatggagt atgcggccgg cgagctgctc gcgcgccacg actggaaagt cgactatgtg
     4561 gttgtgcgta cccggcgcgg actgcgcccc gaagcgcagg acaagaaact ggtggtgctc
     4621 ggcgccgcga agctcggcac cacacggctg atcgacaatc aggaaatcct tgcgccataa
     4681 atcgaggctg aaccacggag gacacagagg acacggagga aaggcaagag caggaaaata
     4741 ggttcgggta aaaacctttc gttggctcat gatccaccgc agcgaaactt ggcggcatag
     4801 gtttgcgaac agtcatcatg gttttttcca caatttgcct ctcctcgttt ttgattttcc
     4861 tccgtgtcct ctgtgtcctc cgtggttcag gaattcgctc tgtgactatt ctctattaca
     4921 gcgaggcaga tccggcggag ccgtggcgcg aggttttcgc gcgcgaactg ccgcgatttg
     4981 cgtttcgtgt ctggccggat tcgggtccgc tggaaagtgt ccggtacgcg ctggtgtgga
     5041 aacccccgcg gggatttctc gcgcagtttc cgaatctcaa ggcgatcctc gcactcggtg
     5101 ccggtgtcga tcccattcct accgatcccg ctttgccgaa agacgttccg ctgttgcggc
     5161 tggtggacgt gggcttcccg gcgcagatgg cggaatacgc gatgtacgcc gtgctgcact
     5221 ttcagcgccg catgggcgat atcgaggccc tgcagcgaca agcccgatgg aaccagctag
     5281 atccattctt cgtccgcgac tttccggtcg gcgtgatggg gctgggcgtc atcggcgcgg
     5341 tcgtggcgca gcgcttcgcg gcagccggct acgcagtggc ggggtggacg aggggcccca
     5401 gacaggtcga tggcatcgac gtgtttgcgg gaagcgagac cttcaggaag tttctcgcac
     5461 gctcccgcgt cgtagtcaat gcgctgccgc tgacgccgca gaccgagagc gtcctcgacg
     5521 ctgcggcatt cgccgcgatg cccaagggag ggtacgtcgt gaacatcggc cgtggcgcac
     5581 atgtggtcga ccgggacctg atcgctgctc tcgatggcgg tcaactggct ggcgcaatgc
     5641 tggacgtgtt ccgcgaggag cccctgcctt cttcgcaccc gttctggcgt catcccaaga
     5701 tcgtcgtcac gccgcatgtc gcggcgccga ccattgcttc cgaagtccag gcgcaggtga
     5761 tcgaaaacat cag
//
LOCUS       DASQOP010000315         2882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_969251, whole genome shotgun sequence.
ACCESSION   DASQOP010000315 DASQOP010000000
VERSION     DASQOP010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969251"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..561)
                     /gene="trmL"
                     /locus_tag="VE030_12410"
     CDS             complement(91..561)
                     /gene="trmL"
                     /locus_tag="VE030_12410"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HZF24255.1"
                     /translation="MFNIVLYRPEIPPNTGNVIRLCANTGCRLHLVEPLGFRLEDRQL
                     KRAGLDYHEYATVQVHPGWDAFLATVPSSRRFAFSTKAANRYDRVEYQAGDVLVFGPE
                     TSGLPEEVRASFEPRRRLRLPMVPTSRSLNLSNAVAAVVYEAWRQSGFTGASSS"
     gene            668..2392
                     /locus_tag="VE030_12415"
     CDS             668..2392
                     /locus_tag="VE030_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HZF24256.1"
                     /translation="MARPEKIRLGEILLQQKLLTSEQLKSALDEQKTSGRRFGRVLID
                     KGFVTEEQISEALARQLNIPYINLKFYNVSREVMLKLPESAARRFRALVLEDAGATYK
                     VGMADPTDITAYDEIVRILRRDIDIVVVMGSELMHTIDRIYRHTEEISGLAQELGVEL
                     GDAIDFAALGITAGAEDAPVVKLLQSVFEDAVQVRASDIHIEPQEKKLLIRFRIDGVL
                     HIQAESEPKIAPALVLRLKLMSGLDISEKRLPQDGRFNVRVRNAQIDVRISTMPTQHG
                     ESAVMRLLNQSGGLLGLERIGMPEPMLEKLRQIIHRPSGMVLVTGPTGSGKTTTLYAA
                     LDELNTPERKIITVEDPVEYRLSGINQVQIHEKIELTFARVLRTALRQDPDVILVGEM
                     RDQATVETGLRAAMTGHLVLTTLHTNDAVSTPIRLLDMGAPRYMVAMSLQVVVAQRLV
                     RIICESCAEPYSLLASEHEWMRNYTGIATDGYTYLKGRGCSQCNGTGYLGRTGVYEML
                     EMTRPVVEAANQPEPARFVEAARAQMEGRMLKDHAVQLVISGRTTVDEAMRVSALLEE
                     GLDLLPPS"
     gene            2445..>2882
                     /locus_tag="VE030_12420"
     CDS             2445..>2882
                     /locus_tag="VE030_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZF24257.1"
                     /translation="MAHFAYKGRNASGELVQGVAEGADSAAVATQLFATGVTPVEIGE
                     TRPLQTGESIGMLQRLTQRPVAPEDLLLFSRQMYALLKAGVPILRALAGLQESTANPT
                     FGRVLQNIRENLDSGRPLSVALQRQERVFSPFYVAMVYVGETTG"
BASE COUNT          584 a          893 c          928 g          477 t
ORIGIN      
        1 cgcggtctgc cgcgttctgg acggccacgc ctctgcccgc gatgcggtcg aagagctgct
       61 gcagcgcgag cccaagcccg aaagtatgcg ctaactgctg ctggcacccg tgaagccgct
      121 ctgacgccac gcttcataga ccacggccgc caccgcattg gacagattca gactgcggct
      181 ggttggcacc atcggcagcc gcagacgtcg tcgcggctcg aagcttgctc ttacctcctc
      241 cggtaaacca ctcgtctccg gaccgaaaac cagcacgtcc cccgcctgat attcgacccg
      301 gtcgtagcgg ttggcagcct tggtactgaa ggcgaaccgg cggctcgaag gaacagtggc
      361 tagaaaggcg tcccagcccg ggtgcacctg gaccgtggcg tattcgtgat aatccaaccc
      421 ggcgcgcttc agttgacggt cctccagccg gaatcccagc ggctcgacca ggtggagccg
      481 gcagccggta ttggcgcaca gccggatcac gttaccagtg ttgggcggga tttcaggccg
      541 atacaggacg atattgaaca tttcgcttgc acttgaaata gttacagttt aatctgctaa
      601 ttcaagaggc tgcaaataaa acctcaagtg acattgaaag tgcggatcag gggaactgcc
      661 aaccggaatg gctagaccgg aaaaaatcag gctgggggaa atccttctcc agcagaagct
      721 gctcaccagc gagcaactca agagcgcgct cgacgagcag aagacaagcg ggcgccgttt
      781 cggtcgcgtg ttgattgaca aggggttcgt aaccgaggag cagatctcgg aagcgctcgc
      841 ccgccagctc aatatcccct atatcaacct caagttctac aacgtcagcc gcgaggtgat
      901 gctcaagctg ccggagtcgg cggcgcgccg cttccgcgcg ctggtgctcg aggacgccgg
      961 cgcgacctac aaggtcggca tggccgatcc cactgacatc acggcttacg acgagatcgt
     1021 gaggatcctc aggcgcgaca tcgacatcgt cgtggtcatg ggatccgagc tgatgcacac
     1081 gatcgatcgc atctaccggc acaccgagga gatcagcggg ctggcgcagg aactgggcgt
     1141 agaactgggc gacgcgatcg attttgcggc cctcggcatt accgcgggcg ccgaggatgc
     1201 gccggtggtc aagctgctgc aatcggtgtt cgaggacgcg gtgcaggtgc gcgcctcgga
     1261 catccacatt gagccgcagg aaaagaagct gttgatccgc tttcgcatcg acggggtgct
     1321 gcacatccag gcggagtctg aaccgaagat cgcgccggcg ctggtgttgc gactgaagct
     1381 gatgtcggga ctggacatct cggagaagcg cctgccgcag gacgggcgct tcaacgtgag
     1441 ggtccgcaac gcccagatcg acgtgcgcat ctcgaccatg cccactcagc acggggaatc
     1501 cgcggtcatg cgcctgctca accagagcgg cggcctgctt ggcctggagc gcatcggcat
     1561 gccggaaccc atgctggaaa agctgcgcca gatcattcac cggcccagcg gcatggtact
     1621 ggtcaccggg ccaaccggca gcggcaagac caccacgctg tacgccgcac tcgacgaact
     1681 caatacgccg gaacgcaaga tcatcaccgt ggaggacccg gtcgagtacc gcctctccgg
     1741 aatcaaccag gtgcagatcc acgagaaaat cgaactgact ttcgcgcggg tgctgcgcac
     1801 cgcgctacgt caggacccgg acgtgattct ggtcggcgaa atgcgcgacc aggccacggt
     1861 ggaaaccggg ctgcgagcag caatgaccgg ccacctggtg ctgaccacac tgcacaccaa
     1921 cgacgcggtc agcacgccga tccggctgct ggacatgggg gcgccccgtt acatggtcgc
     1981 catgtcgctg caagtcgtgg tggcccagcg tctggtgcgc atcatctgcg agagctgcgc
     2041 ggaaccctat tcgctgctgg caagcgagca cgagtggatg cgcaattaca ccgggatcgc
     2101 gaccgacgga tatacctacc tcaaagggcg cggatgctcg caatgcaacg gcaccggcta
     2161 tctgggccgc accggggtgt acgaaatgct ggagatgacg cggccggtgg tcgaggccgc
     2221 caatcagccg gagccggcgc gcttcgtcga ggcggcacgc gcgcagatgg aaggacggat
     2281 gctgaaggat cacgcggtgc agttggtgat cagcggacgc accaccgtcg acgaggcgat
     2341 gcgcgtcagt gcgctactgg aagaaggact tgatttacta ccaccgagtt gatcaacatg
     2401 cagatcgaga gaatccccgt gtccgccgtg gtgagaccct agcagtggct catttcgcct
     2461 acaagggcag aaacgcctcc ggcgagctgg tgcagggcgt ggccgagggc gccgacagcg
     2521 ccgcggtcgc gacccagttg ttcgccaccg gggtcacgcc ggtggagatc ggcgagacgc
     2581 ggcccctgca gacgggtgaa tcgatcggca tgttgcagcg gctgacgcag cgccccgtcg
     2641 cccctgagga cttgctgctg ttcagccgcc agatgtacgc gctgctcaag gccggcgtgc
     2701 cgatcttgcg tgcgctcgcg ggactgcagg agtccaccgc caatccgact ttcgggcggg
     2761 tgctgcagaa cattcgcgaa aacctggaca gcggccgtcc gctgtcggtg gcgctgcagc
     2821 gccaggagcg cgtgttttca ccgttctacg tggccatggt gtacgtcggc gaaaccaccg
     2881 gc
//
LOCUS       DASQOP010000316         4970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_363954, whole genome shotgun sequence.
ACCESSION   DASQOP010000316 DASQOP010000000
VERSION     DASQOP010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4970
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363954"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..1333)
                     /gene="fabF"
                     /locus_tag="VE030_12425"
     CDS             complement(95..1333)
                     /gene="fabF"
                     /locus_tag="VE030_12425"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009913173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HZF24258.1"
                     /translation="MSRRRVVVTGLGTVCPVGNSVKEAWTNIQAGKSGITRITRFDAS
                     PFASQIAGEVKNFDVGQVLSPKEARRVDVFIHYGIAAAVEAIRDAGLEAHPKNAERIG
                     LNIGSGIGGLTIIEETHRAMIEGGARKISPFFIPSAIINMISGNISIMYGFKGPCLAM
                     VTACTTANHCLGDSARLIEYGDADIMVAGGSEAAICSLGIGGFSAARALSTRNDDPAT
                     ASRPWDKDRDGFVLGEGAGVLVLEELEHARSRGAHIYCELAGYGVSADAHHITAPCED
                     GEGAARGMLNAMRNAGINPDQVDYINAHGTSTPLGDMAETIAVKRCFGGHAKRLAISS
                     TKSATGHLLGAAGGVEAIFSVLAIRDQVAPPTINLFNQDPQCDLDYVPNTARSMKIRV
                     AMSNSFGFGGTNGTLIFRTV"
     gene            complement(1367..1603)
                     /gene="acpP"
                     /locus_tag="VE030_12430"
     CDS             complement(1367..1603)
                     /gene="acpP"
                     /locus_tag="VE030_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HZF24259.1"
                     /translation="MESVEQRVKKIVAEQLGVNEADVKNESTFVDDLGADSLDTVELV
                     MALEEEFECEIPDEEAEKITSVQQAIDYIKAHLN"
     gene            complement(1726..2466)
                     /gene="fabG"
                     /locus_tag="VE030_12435"
     CDS             complement(1726..2466)
                     /gene="fabG"
                     /locus_tag="VE030_12435"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HZF24260.1"
                     /translation="MLAGKVALVTGASRGIGQATAVALGRVGATVVGTATSAAGAAAI
                     SAYLADEAIKGAGFKLNVNDPAVMEQVLGNIESQFGSVAILVNNAGITRDNLLLRMKD
                     SEWDEIMDTNLKSVYRLSKLVIRSMMKARFGRIINITSVVGSMGNPGQVNYAAAKAGI
                     AGFSKSLAREVGSRNITVNCVAPGFIDTDMTRGLPEESKTALLAHVPLARFGQPQEVA
                     AAVVFLASEGAAYVTGSTLHVNGGMYMD"
     gene            complement(2496..3428)
                     /gene="fabD"
                     /locus_tag="VE030_12440"
     CDS             complement(2496..3428)
                     /gene="fabD"
                     /locus_tag="VE030_12440"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006759768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HZF24261.1"
                     /translation="MRIAFVFPGQGSQSVGMMQSFAELPGVHESFAQASDLLGQDLWK
                     LAAEGPADELNLTTNTQPVMLVAGVALYRAWQARQGSAPAIMAGHSLGEYTALVAGGA
                     LEFADAVPLVRFRARAMQEAVPAGVGAMAAILGLDDDTVRALCSEASRTNEVVEAANF
                     NSPAQVVIAGNKAAVERGMQIAEARGAKRAVLLPMSVPSHCSLMQPAAKRMRQELGSR
                     AFRRGTVPVLHNADVKSAESPDAIKDALVRQMVQPVRWVETVRQMVSDGVTHIVECGP
                     GKVLAGLNKRIAPESKILSFSDGAAFVQALSVLK"
     gene            complement(3439..4395)
                     /locus_tag="VE030_12445"
     CDS             complement(3439..4395)
                     /locus_tag="VE030_12445"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HZF24262.1"
                     /translation="MNSRIVGTGSYLPEKILTNADLEKIVDTSDEWIRSRTGIRSRHI
                     AADGELTSDLALPAAQKALQSAGVAAGDLDMIIVATTTPDMIFPSTACILQSRLGIAG
                     CPAFDLQAVCSGFVYALGTADLFIRAGQAKKVLVVGAEVYSRILDWNDRGTCVLFGDG
                     AGAVVLAASNTPGILATRLHADGSYKDHLSVPGSVCGGKVWGRPFVHMDGGSVFRFAV
                     RVFEEVAHEVLDQAGLKVSDLDWFVPHQANIRIMEATARRLGLPVEKVIATVDHHGNT
                     SAASIPLALDEAVRDGRIRPGQTLLLEGVGGGFTWGAVLLRW"
     gene            complement(4395..>4970)
                     /locus_tag="VE030_12450"
     CDS             complement(4395..>4970)
                     /locus_tag="VE030_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HZF24263.1"
                     /translation="NVDCSAEHLLQFGAMGSSLVSAVERVERPTVGLLNIGEEEIKGN
                     EVVKQAAELMRDSSLNFYGNIEGTDIYKGTTDVIVCDGFVGNVALKTSEGLAQMLAMY
                     LRQEFGRNLFTKLSGAMALPVINAFRRRVDPRRYNGASLLGLKGIVVKSHGSADSFGF
                     GFAIQRAYDEAHNDMLHGISERMASIHQRVA"
BASE COUNT          918 a         1631 c         1443 g          978 t
ORIGIN      
        1 cacctcaacg gcatattcgc tatctttcag aagacccgcg gcggtcgcca gctcagccgg
       61 ccggctccgt cgggggatgc ttttccggga gcgttcagac cgtgcggaaa atcagcgtgc
      121 cgttggttcc gccgaatccg aaagaattcg acatggcaac cctgattttc atcgatcgtg
      181 cggtgttcgg cacgtaatca aggtcgcact gggggtcttg attgaacaaa ttgatggtcg
      241 gtggggccac ctggtctcga atggcgagca cggagaaaat cgcttccacc ccgccggcgg
      301 cgccgagcag gtgtcccgtc gccgatttgg tcgaactgat tgcgagacgc ttggcgtgcc
      361 cgccgaaaca acgcttcacc gcaatggtct cggccatatc gcccagcggc gtcgaagtgc
      421 cgtgggcatt gatgtaatcc acctggtccg gattgatgcc ggcattgcgc attgcattga
      481 gcatgccgcg cgcggcgccc tcgccatcct cgcagggcgc ggtgatatgg tgcgcatcgg
      541 cgctcacgcc gtaaccggca agctcgcagt agatgtgcgc gccacgcgac cttgcgtgtt
      601 ccagttcctc cagcacgagg actccggcgc cctcgcccag cacgaagccg tctcgatcct
      661 tgtcccaggg tcggctcgcg gtcgccggat cgtcgttgcg cgtgctgagc gcgcgcgccg
      721 ccgagaatcc tccgataccc agtgaacaaa tcgcggcctc ggacccgccg gcaaccatga
      781 tgtcggcatc cccgtactcg atcagtcgcg ccgaatcgcc caagcaatga ttcgcggtcg
      841 tgcacgccgt gaccatcgcc aggcacggcc ccttgaaacc gtacatgatc gaaatatttc
      901 cggagatcat gttgatgatc gcgctaggga tgaagaacgg ggagatcttg cgcgcaccgc
      961 cttcgatcat ggcccggtgg gtttcctcga ttatcgtcag cccgccgatt cccgaaccga
     1021 tattcaggcc gatgcgctcg gcattcttcg gatgggcctc gagtccggcg tcgcggattg
     1081 cctccaccgc agcggcaatg ccgtaatgga tgaaaacatc cacacgccgc gcctctttcg
     1141 gcgacagcac ctggccgacg tcgaaattct ttacctcgcc ggcgatctgg gatgcgaaag
     1201 ggctggcgtc aaaacgggtg atccgggtga tcccggactt gcccgcctgg atgttggtcc
     1261 aggcctcctt gacgctgttg ccgaccgggc aaacggtccc caggccggtg actacgactc
     1321 ggcgcctaga caacgggact ccagaagaag aggaaaaaag gcgtggttag ttgaggtgcg
     1381 ccttgatgta atcaatggcc tgctgaacgg aggtgatctt ttccgcctct tcgtcgggga
     1441 tctcgcactc gaactcttct tcaagcgcca tcaccagttc gacggtgtcg agggaatcgg
     1501 ctccgaggtc gtcgacgaac gtcgattcat tcttcacgtc cgcctcgttc acgcccagct
     1561 gctcggcaac gatttttttc acgcgctgtt ctacggattc cattaatgtc ccttcccttt
     1621 acttgaagat gcagttacgt gtctgattgc cgcccgcccc ggaggggcac ccgcaacggc
     1681 gaaaaacacg cgcgttagtc taccaaaatt gcagggcttc cggagtcaat ccatgtacat
     1741 gccaccattg acatgcaggg tacttccggt gacgtaggcg gcgccctcgg aggcgagaaa
     1801 cacgaccgcc gccgccactt cctgcggctg cccgaatcgg gccaacggca cgtgcgccag
     1861 cagcgcagtc ttgctttcct ccggcaatcc gcgcgtcatg tcggtgtcga tgaatccggg
     1921 cgccacgcag tttacagtga tgttgcggct acccacttca cgggcaagcg acttgctgaa
     1981 tcccgcgatt ccggccttgg ctgcggcata attcacctgc cccgggttgc ccatggaacc
     2041 caccaccgaa gtaatgttga tgatgcgccc gaatcgggct ttcatcatgg agcggatcac
     2101 cagcttactc aagcggtaca cagatttcag gttggtgtcc atgatttcgt cccattcgct
     2161 gtccttcatt cgcagcagca ggttgtcgcg ggtaattcca gcattgttca ccaggattgc
     2221 gaccgaacca aactgcgact cgatgttgcc gagcacctgt tccatgactg cgggatcgtt
     2281 gacattgagc ttgaacccgg caccctttat ggcttcgtcg gccagatagg cggaaattgc
     2341 cgcggcgccc gcggcgctgg tggcggtacc caccaccgtc gcgccgaccc tgccgagcgc
     2401 aaccgcggtt gcctgcccga tcccccggct ggcgcctgtt acgagcgcaa cttttcctgc
     2461 gagcatgaac tcctccccgt agatctgttg caggcctatt tcagtacgga cagggcttgc
     2521 acgaatgctg cgccgtcgct gaatgaaaga atcttggatt ccggcgcgat gcgcttgttc
     2581 agtccggcca gcaccttgcc gggcccgcat tcgacgatat gggtaacgcc gtccgacacc
     2641 atctgccgca ccgtctccac ccagcgcacg ggctgaacca tctgccgaac cagcgcgtcc
     2701 ttgatcgcgt caggcgattc ggcactcttg acatcggcat tgtgcagaac cgggaccgtg
     2761 ccgcgcctga atgcgcggga gcccagttct tggcgcatac gctttgccgc tggctgcatg
     2821 agactgcaat gcgacggcac gctcatgggc aggagcaccg cacgtttcgc tcccctggct
     2881 tcagcgattt gcattccacg ttcgacggcg gccttgttgc cggcgatgac cacctgcgcg
     2941 ggtgaattga agttcgcggc ctcgaccacc tcgtttgttc tgctcgcctc cgaacataac
     3001 gcgcgcaccg tgtcatcgtc cagaccgagt atcgccgcca ttgcgcccac gccggcgggc
     3061 acggcttcct gcattgcgcg ggcgcgaaac cgcaccagcg gcaccgcatc ggcaaattcc
     3121 agcgcaccgc ctgccaccag ggccgtgtat tctccgaggc tgtgccccgc catgatcgcc
     3181 ggcgccgatc cttgccgcgc ctgccacgca cgatacagcg ctacgcccgc cactagcatc
     3241 accggctgcg tattggtggt gagattgagt tcatccgcgg gaccctcggc agcaagcttc
     3301 cacagatcct gccccagcag gtcggaagcc tgcgcaaacg attcgtgcac gcccggcagt
     3361 tctgcgaagg actgcatcat gccgaccgac tgcgagccct gccccggaaa gacgaaagca
     3421 atcctcatgt cgacgcaatt accagcgcag caacaccgcg ccccaggtaa atcctccgcc
     3481 cacgccttcc agcagcagcg tttgcccggg gcggattcgc ccgtcgcgca ccgcttcgtc
     3541 gagcgccagc gggatcgagg cagcagaagt attgccgtga tgatccacgg tggcgatgac
     3601 tttctcgacc ggcagtccga gtcgtctggc ggtcgcctcc atgatgcgga tgttggcctg
     3661 gtgcggcacg aaccagtcca ggtccgacac cttcaatccg gcctgatcca gcacttcgtg
     3721 cgcgacctcc tcgaataccc gcacggcgaa cctgaaaacc gagcccccgt ccatatggac
     3781 gaacggccgg ccccacacct tgcccccgca tacgctgccc gggacggaaa gatggtcctt
     3841 gtagctgcca tcggcgtgca gccgtgtggc aagaatccct ggtgtgttgc tcgccgcgag
     3901 cacgaccgca cccgctccgt cgccgaacag cacgcaggtg ccacgatcgt tccagtcgag
     3961 aatccgggag tagacttcgg cgcccaccac cagcactttc tttgcctggc cggcgcgaat
     4021 gaaaagatcc gccgtaccca gcgcatagac gaaaccgctg cacaccgcct gcaggtcgaa
     4081 cgccgggcag ccggcaatgc caagcctgga ttgcaggatg catgcggtac tcgggaaaat
     4141 catgtccggc gtggtggtcg cgacgatgat catgtcgagg tcgcccgccg cgacacctgc
     4201 ggactgcagc gccttctgcg cagccggcag cgccagatcg ctggtcagct caccatccgc
     4261 cgcgatatgg cggctgcgaa tgccggtgcg gctgcggatc cattcatcgg aggtgtcgac
     4321 gattttttcc agatcggcgt tggtgagaat cttctcggga agatagctgc ctgtgccgac
     4381 gatccgggaa ttcatcacgc caccctttga tgaattgagg ccatgcgctc gctgatgccg
     4441 tggagcatgt cattatgcgc ttcgtcgtag gcccgctgga tcgcgaaacc gaaaccgaaa
     4501 ctgtctgccg aaccgtggct cttgaccacg atgcctttca gaccgagcag actggcgcca
     4561 ttgtagcgac gaggatcgac gcggcggcgg aaggcattga tgacgggcaa agccattgcg
     4621 ccagagagct tggtgaacag attgcggccg aactcctggc gcaggtacat cgccagcatc
     4681 tgcgccagcc cttccgaggt cttgagcgcg acgttgccga cgaagccatc gcatacgatc
     4741 acgtcggtgg tgcccttgta gatgtcggta ccctcgatgt tgccgtagaa attgaggctg
     4801 ctgtctcgca tcagttccgc ggcctgcttg accacttcgt tccccttgat ttcctcttca
     4861 ccgatgttga gcaggcccac cgtcggacgc tcgacacgtt ccacagcgct caccagcgac
     4921 gatcccatcg cgccgaactg cagcagatgt tccgcgctgc agtccacatt
//
LOCUS       DASQOP010000317        14278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2584190, whole genome shotgun sequence.
ACCESSION   DASQOP010000317 DASQOP010000000
VERSION     DASQOP010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14278
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2584190"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VE030_12455"
     CDS             complement(<1..434)
                     /locus_tag="VE030_12455"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HZF24264.1"
                     /translation="MQHIVNPAKELLSKGAIAIGIGVRAVRGIEIARVMKTAGFDWLF
                     IDLEHGPTSVETAYSIAVAALDAGIAPLVRVPAGELALATRCLDGGALGIVMPHVDTP
                     EQARAMVDAFRFTPLGHRSIAGGYPQFGFAAAPPSEVVAEMN"
     gene            complement(537..1541)
                     /locus_tag="VE030_12460"
     CDS             complement(537..1541)
                     /locus_tag="VE030_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZF24265.1"
                     /translation="MKKVVWGILGTAKIGRQRVIPGMQKSKWCEIRAIASRSAQSARA
                     AAGALGIPKAYGSYEELLADPDIEAVYNPLPNHLHVPLTLRAAAAGKHVLCEKPVALN
                     AEEAAQLRAVAGKVLIMEAFMVRFHPQWLRVREHIRDGRIGALRVLQMLFSYFNVDAG
                     NIRNIAAIGGGALYDIGCYPITAGRFFFESEPQRAIALVDRDPGFGTDRMTSALLDFG
                     SGRHLDFTVSTQCAPYQRIQLSGSKGRIEIQIPVNAPQGEKTSIFLDDGSSLAGAGIR
                     TETLPESDQYTLQGEAFSRAIRGEISLPYGVEDAVQSMRVIDALFRSEKSGRWEEVQG
                     "
     gene            1684..2163
                     /locus_tag="VE030_12465"
     CDS             1684..2163
                     /locus_tag="VE030_12465"
                     /inference="COORDINATES: protein motif:HMM:NF020652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1924 domain-containing protein"
                     /protein_id="HZF24266.1"
                     /translation="MKRTILLFVFAIWASPGLGASPEELLSAYAAQAGQVDPGFAGFS
                     ADRGKAFYFSGHRVEDGSEFSCASCHHEDPRREQFAHHDKIPCRACHGMPDSGSFEDI
                     PKIRRQFLPLAPSANSRRFTDEWFVEKWFRKNCGLLLKRDCTPVEKGDVMTWLLTFK"
     gene            complement(2172..2765)
                     /locus_tag="VE030_12470"
     CDS             complement(2172..2765)
                     /locus_tag="VE030_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HZF24267.1"
                     /translation="MGNSSTVASADQVVAATRAWLEKAVIGLNLCPFAKTVHVANQIR
                     YAVSNAQTTEALLADLGCELQTLAAADPAQVETTLLVHPRVLDDFLDYNDFLDIADAA
                     VEELGLTGTIQVASFHPQYRFADTEPDAIENYSNRSPYPMLHLLREESVERAVATFPH
                     AGHIPERNIETLRRIGHDGWRRLGLPAPPEPAGKTRK"
     gene            complement(2845..3036)
                     /locus_tag="VE030_12475"
     CDS             complement(2845..3036)
                     /locus_tag="VE030_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24268.1"
                     /translation="MADEFQMDEVDKLAAQLFVQRLGAWVATGRQDRIRDTEISAAAT
                     NAYQEADAFIKIFLERKKR"
     gene            3152..3397
                     /locus_tag="VE030_12480"
     CDS             3152..3397
                     /locus_tag="VE030_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24269.1"
                     /translation="MRRIRKPKPSGVCNVCGQLTDQIEMINHRCTYTLHGRRCAGLFK
                     SELNHIWVACESCDAVGKVGTVICSECSGFGWRLLGA"
     gene            complement(3491..4732)
                     /locus_tag="VE030_12485"
     CDS             complement(3491..4732)
                     /locus_tag="VE030_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HZF24270.1"
                     /translation="MERITVAEMAATTVALSFAFAACAADAIRIAHIDPMSGPFGLVG
                     ESLGKHLDATAAEINARGGVPGGMKFEIVHFDNKSSPQESVLLLKQVTDSGIRFVTQG
                     GGSNIAHALSEAVAKHNSRNPDSSVLYLNYAAQDPTLTNDKCNFWHFRFDAHVDMKLD
                     AITNHIAQQNDIKKVYLLNQDYAFGQAISKAARGMLTRKRPDIEIVGDDLHPLGKVKD
                     FSPYIAKIKASAAQAVITGNWGADFTLLIKSSKESGLGVTFYTFNAQNAGAPSSVGAA
                     GADHIKQVFTWHANIADNKAEKFALDFRKKYNGDFYYNTAKTELEMLAKAMIDTKSID
                     PLKVARALEGMKYQSDTGEVWMRADDHQLMQPIYIATFTKAGGKDVKIDAEGTGFGWK
                     TDIRIEPADTVMPTTCRMERP"
     gene            complement(5031..5909)
                     /locus_tag="VE030_12490"
     CDS             complement(5031..5909)
                     /locus_tag="VE030_12490"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HZF24271.1"
                     /translation="MPVQSQKQHRWPFDVRPLTPKLGAEIIGVDLARGVDDALFRSIH
                     QAFLQYQVLLFGTQDLPPGRQVEFSRHFGEVQIHVMNQYRADGHPELYRLSNLDENGN
                     PNGKHPDKGTLAWHTDGSWRRVTGQATIIYAEVVAGEGGETHFCDMYGAYQRLSPEWK
                     RRIANLRAVHNLDFSRTRRHGEDPMTEAQRREVPPVDHPIVRTHPDTGRKCLFLGDHA
                     EYIAGMDYDEGRALIDELNALAVHPDLTYEHRWTPGELIVWDNRCLMHRATDYDPAVQ
                     RRVVRRCTVLGEIPRQ"
     gene            complement(6016..7341)
                     /locus_tag="VE030_12495"
     CDS             complement(6016..7341)
                     /locus_tag="VE030_12495"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZF24272.1"
                     /translation="MSARRITNATLSTAILFVALLSFAAAWAQGLPAAPAESVGMSAQ
                     KLGRIAETFRKEIDLGKLPGAVFLVARKGKLAYSDAIGLQNKETGRPMPKDAIFRIYS
                     MTKPIVSVAAMMLVEDGRVQLTDPVSKFLPAMKNPQVSVAKTDAEFAKVMYTLVPTDR
                     EMTVQDLLRHTAGLAYGELTQNTPVKEALGKAGIYKTTIDFDSRDLPPAEQVERMSKV
                     PLAHQPGTVWEYSLASDLLGRVVEAASGERLADFLERRLFKPLKMNDTGFWVPKEKLG
                     RLAEPLLADAATGKASNLMDVSAVPANDSGGAGGVSTAADYLRFSQMLLNGGQLDGAR
                     VLSRSSVALMASDHLGTRIAAPFTPGELLLGTPGYTFGLGFAVRQGAGIAALPGSAGE
                     FMWGGYAGTYFWVDPKEEIVAVYMVQAPGPMRPYYRRLFKQLVYAAIND"
     gene            complement(7906..10203)
                     /locus_tag="VE030_12500"
     CDS             complement(7906..10203)
                     /locus_tag="VE030_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HZF24273.1"
                     /translation="MNNWIQNVSKPGRRDFLKGAAATGFVVGLQMAPVAKTGPFSGLG
                     GIGNADAAMGTLSPNVFVSIDKSGTVTIIAHRSEMGQGVRTSVPMILADELEADWSRV
                     KVEQAVGDKMYGNQYTDGSRSVRHNFQRMREFGATTRAMLEQAAAVKWNVPVTEVEAK
                     NHKVVHLPTKKSADYGELVEIAATLKVPDAKRVQLKDPKEWRYIGKEIVGVDNADMVV
                     GKATYGIDTKLPGMKYAVVEHCPVTYGKVKSFDASEAMKVPGVEKVVEIPQNQPPIVF
                     NTLGGIAVIANNTWSANEGRKKLKIDWDMGPNAVYNSDSFRQGMEEEARNGTGAKVVR
                     KEGDADAALAKAAKVVEATFYVPHFAHATMEPPAAIAVVKGGKCEVWSACQDPQAARA
                     TVAGAIGMDEANVTSHATLLGGAFGRKSKPDFAAEAAILSKMLGGTPVKVTWSREDDI
                     QHAYYHTVCAQHVKAGLDASGKAIAYHHRTVFPTIWSSFEKEDPQYGHKIELSLGFLE
                     VPYNVPNLTLENGKAKAHVRIGWLRSVNNIPHAFAQNSMANMMAYAAGKDPYQYLMDL
                     LGPDRVLDLSNTDYWNYDQTYDEYPIMTARLKNVLKVAAEKAGYGRKLGDREAIGVAV
                     HRSFTTYVATAVRVQVSKDGKISIPRVDVACDAGLVVNPDRVRSQMEGACIYGMTGAM
                     YGSITAKEGVIEQSNFHDYQMVRMPEAPREIHVHILNAAGNFPPGGVGEPGVPPFTPA
                     LTNAIFAATGKRIVDLPISKHNLAA"
     gene            complement(10200..10655)
                     /locus_tag="VE030_12505"
     CDS             complement(10200..10655)
                     /locus_tag="VE030_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZF24274.1"
                     /translation="MAISLNVNGKAQQVDVPEEMPLLWVLRDTLGLTGTKFGCGMAQC
                     GACTVHVNGMAMRSCALPVGSVSGPVVTIEGLSRNGDHPLQKAWVKNNVPQCGYCQAG
                     QIMQAAALLKNTPKPSDEDIEGAMSGNICRCGTYTRIRAAIKEAAGVKS"
     gene            complement(11357..11866)
                     /locus_tag="VE030_12510"
     CDS             complement(11357..11866)
                     /locus_tag="VE030_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24275.1"
                     /translation="MKASRIISLLAAGGLCLAAAGARAAESFSLGGHTLGLPYDAILN
                     DPAYDCESLAGCFLFSACAYKATGAVAFKGTPIDRLTLYFTGERLSGIAASFQESHFD
                     QVAELLAQEYGPGTVEIRKADTGTTSSNAVLVWRQSSKLLRLERSGKSGRSSVILAER
                     NFISELVGP"
     gene            complement(12146..13558)
                     /locus_tag="VE030_12515"
     CDS             complement(12146..13558)
                     /locus_tag="VE030_12515"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HZF24276.1"
                     /translation="MANNDGSGASKASRVEGNRQVEQGNVERRRRMRLPARLVAVSWR
                     DLLVIGVPVLLITAAAAWIAVKFVRPAPPNTIIISAGAPGGSNLATAEKYRKIIEAHG
                     VKVQILESRGSLENLQRLANPAFKLDVAFVQGGLTDGVNITGLMSLGSMFPQPVMVYY
                     RNPEPVERLSQLAGKRLAIGPEGSGTRALVLKLLKANAIDGEPTVLLELAGEEAADAL
                     IAGSVDAAFLTGDSATGKVMRRLRQVPGIELMSFKQANGYVRKFRFLSRLTLPEGSID
                     LGANFPPKNYQLIGPTVELVAREGLHPALSDLLISAAREVHGEPGLFRDAGEFPAPLE
                     RDFPISPDAERYYKSGKQFLYKQLPFWLASLVDRLLVVLVPMLVLIIPVTRIVPPLYR
                     WRVRSRIYRWYGTLMAIERDMLGELTPERREQILKRLDNIEEYVNNIKTPLSFADQLY
                     VLREHISMVRHRIETGTFSH"
     gene            complement(13574..14200)
                     /locus_tag="VE030_12520"
     CDS             complement(13574..14200)
                     /locus_tag="VE030_12520"
                     /inference="COORDINATES: protein motif:HMM:NF016819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HZF24277.1"
                     /translation="MPARLSMPAVRAGVALCLLLAAGCAYAHSANPGAGDFYAGALHA
                     LTALEHVLPFLALGILAGQQGRKAEPVLLVFCLALAAGATVALWIPPPPYVVLLNILS
                     AILFGGLVAAAWSLPMAFCCSIAAVFGLSHGLANGAGMIEQTKPYLYIPGVALAGLAV
                     TAYGLIVTDYLLRRKAGWIHIAVRVAGSWIAAIGILVLATSGAAILKA"
BASE COUNT         2516 a         4461 c         4407 g         2894 t
ORIGIN      
        1 tggttcattt ctgcaacgac ttcacttggc ggtgccgcgg caaaaccgaa ctgcggatag
       61 ccgccggcta tggaccggtg tccgagcggc gtgaatctga acgcatccac catggcgcgc
      121 gcctgttccg gtgtatccac gtgcggcatc acgatgccga gcgcgccacc gtccaggcag
      181 cgggtggcga gcgcaagttc gccggccggc acccgcacca gcggcgcgat gccggcgtcg
      241 agcgccgcca ccgcgatcga atacgcggtc tccaccgagg tcggcccatg ctccaggtcg
      301 atgaacagcc agtcgaagcc cgcggttttc atcactctcg cgatctcgat gccgcgcacc
      361 gcccgcacgc cgatcccgat cgcgatcgcg cccttcgata gcagctcttt tgccggattc
      421 actatgtgct gcattctctc ccccatgtgt tgttatggca tgtgctacgt gctgcgtgtt
      481 gcaacaatac cagccgccca tgaccggctg ttccttcatc cttcatcctt ccgccttcat
      541 ccctgcactt cctcccatct tccgcttttc tcggagcgaa acaacgcgtc gatcacgcgc
      601 atgctctgca ccgcgtcttc cacgccgtag ggcagcgata tttcgccgcg aatcgcgcgc
      661 gagaacgcct ctccctgcaa agtatactgg tcgctctccg gcagggtctc ggtgcgaatg
      721 ccagcacccg ccagcgacga gccgtcatcc aggaaaatgc tggtcttctc gccctgcggc
      781 gcgttgaccg ggatctggat ttcgatccgg cctttggacc cgctgagctg gatgcgctgg
      841 tagggcgcgc actgggtcga aacggtgaaa tccagatggc ggccggagcc gaagtccagc
      901 agggcgctgg tcatccggtc ggtgccgaag cccgggtcgc ggtcgaccag cgcgatggcg
      961 cgttgcggtt ccgattcgaa gaagaaccgg ccggcggtga tcggatagca gccgatgtcg
     1021 tagagggcac cgccgccgat cgcggcgatg ttgcggatgt tgccggcgtc gacgttgaaa
     1081 taggaaaaaa gcatctgcag cacgcgcaac gcgccaatcc ggccgtcacg gatgtgctcg
     1141 cggacccgca gccactgcgg atggaagcgc accatgaaag cctccatgat cagcaccttg
     1201 ccggccacgg cgcgcagttg tgcggcttct tcggcgttga gcgcaacggg tttctcgcac
     1261 agcacgtgct tgccggctgc cgcggcccgc agcgtcagcg gcacatggag atggttgggg
     1321 agcgggttgt aaacggcttc gatatcgggg tcggcgagca attcctcgta agacccgtaa
     1381 gccttcggga taccgagcgc gcccgcggct gcgcgcgccg attgcgctga acgggaagcg
     1441 attgcgcgga tctcgcacca cttgctcttc tgcatgcccg gaatgactct ctgccgcccg
     1501 attttcgcgg tgccgagtat gccccagacg actttcttca ttttctcctc ccggtggaat
     1561 ttttccggcc gagtgtacct cacggccgca gacatcgtct ccggtacccg tttccgcggg
     1621 gagggtgcaa agtccgccga atcgcttcac aatgacccgc acaatggagg cctgcgagta
     1681 ggcatgaagc gcacgatcct gttgttcgtc ttcgcaattt gggcatcgcc cggtctgggc
     1741 gcgagcccgg aagagttgct gtccgcgtat gccgcgcagg ccggccaggt ggatccgggc
     1801 tttgccgggt tctcggccga ccgcggcaag gcgttctact tttccggcca tcgcgtggag
     1861 gatggctccg agttttcctg cgcgtcctgc catcacgagg atccgcgtcg cgaacagttc
     1921 gcccatcacg acaagattcc ctgccgcgcc tgccacggca tgcccgacag cggtagtttc
     1981 gaggacattc ccaagatcag gcgtcagttc ctgccgttgg cgccttctgc gaactcgcga
     2041 cgcttcaccg acgaatggtt cgtcgagaaa tggttccgca agaactgcgg tctgctgctc
     2101 aaacgcgatt gcacgccggt ggaaaaaggc gacgtgatga cctggctgct tactttcaaa
     2161 taggcgaacg cctacttccg ggttttcccg gccggctcag ggggcgcggg aagcccgagg
     2221 cggcgccagc cgtcatggcc gattctgcgc agggtctcta tattgcgctc cgggatgtgc
     2281 ccggcatggg ggaatgtcgc caccgcgcgc tcaacgcttt cctcgcgcaa caggtgcagc
     2341 atcgggtagg gcgagcggtt gctgtagttc tcgatggcat ccggctcggt gtccgcgaac
     2401 cgatactgcg gatggaaact ggcaacttga attgtgccgg tgaggccgag ttcctcgacg
     2461 gcggcgtcgg cgatatcgag aaaatcgttg tagtccagaa aatcgtcgag cacgcgcgga
     2521 tgaaccagca gcgtggtttc gacttgcgcg ggatcggccg cagccagcgt ctgcaactcg
     2581 cagccgaggt cggctaagag cgcctccgtg gtctgcgcat tgctgactgc gtagcggatc
     2641 tggttcgcga cgtggacggt cttggcgaag gggcacaggt tcaggccaat gacggccttc
     2701 tccagccatg cgcgtgtcgc cgcgacgact tggtctgccg atgcaacggt gctgctgttt
     2761 cccatcagct ttgcagcgac cgtacaaccg tcccgggaaa tcgcatgccg ggtggaaccc
     2821 gcatgatgct gcacgcggaa agcatcagcg cttcttcctc tcgaggaaaa tctttatgaa
     2881 tgcgtccgct tcctggtagg cgttcgtggc ggcggcgctg atctcggtat ccctgatccg
     2941 gtcctggcgt ccggtggcca cccaggcccc cagccgctgg acgaaaagtt gtgcggccag
     3001 tttatcgact tcgtccatct ggaattcgtc cgccatttgt ctctcctcat gagaaggtgc
     3061 gtccgtgcgc aggtcagtgt aacgtagcca attcgccccg ggcgggggga cggccggggc
     3121 gcccctttca attgtctgcg aggcgatgca catgaggcga ataaggaagc ccaagccctc
     3181 gggtgtctgc aacgtctgcg gccaattgac cgatcagatc gagatgatca atcaccgctg
     3241 tacctacacg ctacacggca ggcgctgcgc cggcctgttc aagagcgagc ttaaccacat
     3301 ctgggtggcg tgcgagtcgt gcgacgctgt cggcaaggtc ggaaccgtga tctgcagcga
     3361 gtgttcggga tttggctggc ggctgctggg cgcttaacgc gagtgctgag cgcttcgaga
     3421 gaaacgaaaa aagaacaagg gtcaggcctc aggcctgacc cctggttatt cagttgacgg
     3481 tcactgtcgc tcatggccgc tccatcctgc aagtcgtggg cataaccgtg tcggccggct
     3541 cgatacgaat gtcggtcttc cagccgaaac cggtgccttc ggcgtcgatc ttcacgtcct
     3601 tgccgccggc cttggtgaag gtggcaatgt agatcggctg catcaactga tgatcgtcgg
     3661 cgcgcatcca gacctctccg gtgtcactct ggtacttcat gccttcgagc gctctggcaa
     3721 ctttcagcgg atcgatggat ttggtgtcga tcatcgcctt ggccagcatt tccagctcgg
     3781 tcttggcggt gttgtagtag aagtcgccgt tgtatttttt cctgaaatcc agtgcgaact
     3841 tttccgcctt gttgtcggcg atgttcgcgt gccaggtgaa cacttgcttg atatgatccg
     3901 cccccgccgc accgactgaa ctcggcgcac cggcgttctg ggcattgaac gtgtaaaaag
     3961 tcacgcccag cccggattcc ttgctcgact tgatcagcag cgtgaaatcc gcgccccagt
     4021 tgccggtgat gaccgcttgc gcggcagagg ccttgatctt ggcgatgtaa ggcgaaaaat
     4081 ccttgacctt gcccagtggg tgcagatcgt cgccgacgat ctcgatatcg ggacgttttc
     4141 tggtcagcat cccgcgggct gccttgctga tagcctggcc gaaggcgtag tcctgattga
     4201 gcagataaac cttcttgatg tcgttctgtt gggcgatgtg gttggtgatg gcgtcgagct
     4261 tcatgtccac gtgcgcatcg aagcgaaagt gccagaaatt gcacttgtcg ttggtcagcg
     4321 tcgggtcctg ggcggcataa ttgagataga gcaccgagct gtccggattg cggctgttgt
     4381 gcttggcgac ggcttcggaa agcgcatgag cgatattcga gccgcctccc tgcgtgacga
     4441 accggatccc ggagtcggtg acctgcttga gcaatagcac gctttcctgc gggctcgact
     4501 tgttgtcgaa gtgcacgatt tcgaatttca tgccgcccgg cacgccgccc ctggcattga
     4561 tttccgccgc cgtggcgtca aggtgcttgc cgagactttc cccgacgagc ccgaacgggc
     4621 cgctcattgg gtcaatgtgg gcgatccgga tcgcatccgc cgcacaggcc gcaaacgcaa
     4681 acgacagcgc gaccgttgtg gccgccattt ctgcaacggt gatgcgttcc atggtttttc
     4741 ctcccgaatc aattttgtct tcagttccgc cggtcaccga taacgagcga cgagtgcctg
     4801 cagaattcta tgtgactctc gttttgttct tatccctctt tgcgttcttt cttgcccttc
     4861 tccgcgaaaa tcaaacgcga tgatggaatt gtagttccgg cataacccgt aaacgggtta
     4921 gtctgcactg aaactcgcgg agctcgtttt caacgccctt atcgccggcc cgtggctggc
     4981 tggtttcact gagtcccgag catcaagtcc ggagtcctgg ttcttgtctg tcactgacgc
     5041 ggaatttcgc ccagaaccgt gcagcgccgg accacgcgcc gctgcaccgc cggatcgtaa
     5101 tcggtggcgc gatgcatcag gcagcggttg tcccagacga tcagctcgcc cggcgtccag
     5161 cgatgctcgt aggtgagatc cggatgaacg gcgagcgcgt tgagttcgtc gatgagcgcg
     5221 cggccctcgt cgtaatccat tccggcaatg tattcggcgt gatctcccag gaacaggcac
     5281 ttgcgcccgg tgtccgggtg cgtgcgcacg atcgggtggt ccaccggcgg gacttcacgg
     5341 cgctgcgctt cggtcatcgg gtcttcgccg tggcggcggg tgcgcgaaaa atcgaggttg
     5401 tgcacggcgc gcaggttcgc gattcgcctt ttccattccg ggctcaggcg ctggtaggcg
     5461 ccgtacatgt cgcagaagtg cgtctcgccg ccttctccgg cgacgacctc cgcgtaaatg
     5521 atggtggctt ggccggtgac ccggcgccat gagccgtcgg tgtgccacgc cagcgtgccc
     5581 ttgtcgggat gcttcccgtt cgggttgccg ttctcgtcga ggttggacag gcgatacagc
     5641 tcggggtggc cgtcggcgcg gtattggttc atcacgtgga tctgtacctc gccgaaatgg
     5701 cgggagaatt ccacctgccg tcccggcggc agatcctgcg tgccgaacag cagcacctgg
     5761 tattgcagaa aagcctggtg gatcgagcga aacaatgcat cgtccacgcc acgcgccagg
     5821 tccacgccga tgatctcggc gccgagcttc ggcgtcaacg gccggacatc gaatggccaa
     5881 cgatgctgct tttgagactg cacgggcatg tcagtattat gagtgatgag tgatgagtga
     5941 ttgagtgatt tagtcaccca ttccccattc cccattcccc tttccccatt gcccattacc
     6001 cattccccga atttctcagt cgtttatcgc cgcgtaaacg agctgcttga acagccgacg
     6061 gtagtacggg cgcatcgggc cgggtgcctg caccatgtac accgcgacga tctcttcctt
     6121 cggatccacc cagaagtagg tcccggcgta accgccccac atgaattctc ccgccgaacc
     6181 cggcagggcg gcgatgcccg cgccctggcg caccgcaaac ccgaggccga acgtgtagcc
     6241 gggcgtaccg agcagcagtt cgcccggcgt gaacggcgcc gcgatgcggg tgccgaggtg
     6301 gtcggaagcc atcagtgcca ccgaactgcg gctcagcacc cgggcaccgt cgagctggcc
     6361 accattgagg agcatctgcg aaaaacgcag gtagtccgcc gcggtagaca cgccacccgc
     6421 tccgcctgag tcgttggcag gcactgccga tacgtccatc aggttgctgg ccttgccggt
     6481 ggcggcgtcg gccaaaagcg gttcggccag ccggccgagt ttttccttcg gtacccagaa
     6541 gccggtgtcg ttcatcttca gcggcttgaa caggcgcctt tcgagaaagt ccgcgagccg
     6601 ttctcccgat gccgcctcga ccacgcggcc gagcaggtca ctcgccaggc tgtattccca
     6661 gacggtaccg ggctgatgcg cgagcggcac tttcgacatg cgctccacct gttccgccgg
     6721 cggcaggtcg cgcgagtcga agtcgatggt ggtcttgtag atgccggcct tgcccagcgc
     6781 ctctttcacc ggcgtgttct gcgtcagctc gccgtaggcc aggccggccg tgtggcgcag
     6841 caagtcctgc accgtcattt cccgatccgt cgggaccagc gtgtacatca ctttggcgaa
     6901 ctcggcatcg gtctttgcca cgctgacctg cgggttcttc atcgccggaa gaaacttcga
     6961 gaccggatcg gtgagctgta ccctgccgtc ctcgaccagc atcatcgcgg cgaccgaaac
     7021 gatcggcttg gtcatcgaat agatgcggaa gatcgcgtcc ttcggcatcg gcctgccggt
     7081 ttccttgttc tgcagcccga tcgcatccga gtacgccaac ttgcccttgc gcgccaccag
     7141 gaacaccgcg ccgggcagct tgcccaggtc gatttccttt ctgaaggttt ccgcgatgcg
     7201 gccgagtttc tgcgcggaca tgccgaccga ctcggccggg gctgcgggaa ggccctgcgc
     7261 ccaggcggca gcaaaactta aaagggcgac gaaaaggatt gcggtggaaa gggtcgcgtt
     7321 ggtgatgcgt ctggcgctca tttgcgtttt cctcgatcgt gaatgccgtg ctgccggaag
     7381 gtacgtcgga cgccgggacg gtgtcaatgc ggcgagtccc gcgggcgtgt tcgcgcttgc
     7441 gatctgttcg gattctcggc acaaggtgac cggcgcccgg gtgttgaaga cttgatcgct
     7501 tggcggaaac actcgtcgcc gaaccgttac ggccaagcgt taaccgcaaa ggacgccaag
     7561 gacgctagga aagtcaaagg caagaatcag gtgactgttc aaaccccgtg caagaatctg
     7621 cgttgtgact ggcccgttgc ccgttacccc aactcagcct cgccttcacg ctattgcctt
     7681 tccttggcgt ccctggcggt ggatgcgtta ttggtgaggc gttcgaaacc cacagaaaag
     7741 tctgaaggac caaataaaaa gggcccgcca ttgcgacggg cccttcaatt cactttcatg
     7801 cctgcaggaa ctgcgttccc aaccaagcct gccggcttct tgtcggcctg cgcggggcgg
     7861 gggcgcggcg actgctctgc gccacgcccc cccgacaccc tggaatcagg cagccaggtt
     7921 gtgcttcgag atcggcaggt cgacgatgcg cttgccggtt gccgcgaaga ttgcgttggt
     7981 taacgcggga gtgaacggcg gcacgcccgg ttcgcccacg ccacccggcg ggaagttgcc
     8041 ggcggcattg aggatgtgca cgtgaatctc gcgcggagcc tccggcatac gcaccatctg
     8101 gtagtcgtgg aagttgctct gctcgatgac gccttccttg gcagtaatgc tgccgtacat
     8161 ggcgccggtc atgccgtaaa tgcaggcgcc ttccatttgc gaacgcacac ggtccggatt
     8221 gaccaccagc ccggcatcgc aggccacatc gacgcgcggg atggagatct tgccgtcctt
     8281 cgacacctgc acacgcaccg cagtcgccac gtaagtggtg aaactgcgat gcacggcgac
     8341 gccgatggct tcgcgatccc cgagcttcct gccgtagccc gccttctccg ccgcgacctt
     8401 gagcacgttc ttcagcctcg cggtcatgat cggatactca tcgtaggtct ggtcgtagtt
     8461 ccagtagtcg gtgttagaca gatccagaac gcgatccggc ccgagcaggt ccatcaggta
     8521 ctggtacggg tccttgccgg cggcatacgc catcatgttc gccatcgagt tctgcgcgaa
     8581 cgcatgcggg atgttgttca ccgaacgcag ccagccgatc cgcacatgcg ctttcgcctt
     8641 gccgttttcc agcgtcaggt tggggacgtt gtacggcact tcgaggaatc cgagcgacaa
     8701 ctcgatcttg tggccgtact gcggatcctc cttctcgaac gaggaccaga tggtagggaa
     8761 caccgtgcgg tggtggtaag cgatcgcttt cccgctcgcg tccaggccag ccttgacgtg
     8821 ctgtgcgcac accgtgtggt agtaggcatg ctggatatcg tcctcgcgcg accaggtgac
     8881 ctttaccggc gtcccgccca gcattttcga caggattgcc gcttccgccg cgaagtcggg
     8941 cttcgacttg cggccgaagg cgccgcccag cagcgttgcg tgcgaggtaa cgttggcttc
     9001 gtccatgccg atcgcgccgg ccacggttgc acgtgcggcc tgcggatcct ggcaggcaga
     9061 ccagacctcg cacttgcccc ccttcacgac cgcgattgca gccggcggtt ccatggtggc
     9121 gtgagcgaag tgcggcacat agaaggtggc ttcgaccact ttcgccgctt tcgccagcgc
     9181 tgcgtccgcg tcaccttcct tacgcaccac cttggctccg gtgccgttgc gtgcttcctc
     9241 ttccatcccc tggcggaatg agtccgagtt gtacactgcg ttcgggccca tgtcccagtc
     9301 gatcttcagt ttcttgcggc cttcgttggc ggaccaggtg ttgttggcga tcaccgcaat
     9361 cccgcccagg gtgttgaaca cgatcggcgg ctggttctgc ggaatctcga cgactttttc
     9421 gacgccgggg accttcatcg cctcggacgc atcgaaggat ttcaccttgc cgtacgttac
     9481 cgggcagtgc tcgaccactg cgtatttcat gccggggagc ttggtgtcga tgccgtaggt
     9541 tgccttgccc acgaccatgt cagcattgtc cacgccgaca atttccttgc cgatgtagcg
     9601 ccattccttc gggtccttga gctggacgcg tttcgcatcc ggcaccttca gggtggcggc
     9661 gatctcgacc agttcgccat aatccgccga ttttttggtg ggcaggtgca cgaccttgtg
     9721 attcttggcc tcgacttcgg ttaccggcac gttccacttg accgcggcgg cctgctcgag
     9781 catggcgcga gtggtcgcgc cgaactcgcg catgcgctgg aagttgtggc gcacgctgcg
     9841 cgaaccgtcg gtgtactggt tgccgtacat cttgtcgccc accgcctgtt cgaccttgac
     9901 gcgactccag tcggcttcca gctcatcggc gaggatcatc ggcacgctgg tgcgcacgcc
     9961 ctggcccatt tcggagcggt gcgcaatgat cgtcaccgtg cccgacttgt cgatggaaac
    10021 gaacacgttg gggctcaatg tgcccatcgc cgcgtccgcg ttgccaatgc cgcccagtcc
    10081 ggagaagggc ccggtcttgg caaccggagc catttgcagg ccgaccacga agcctgtcgc
    10141 ggcggcgcct ttcaggaagt cccgacggcc gggtttgctg acgttctgta tccagttatt
    10201 catgatttta cccccgcagc ttctttgatc gccgcgcgaa tccgcgtgta cgtgccgcag
    10261 cggcagatgt tgcccgacat ggcaccttcg atatcttcat cggacggctt gggcgtgttc
    10321 ttcagcagag ccgcggcctg catgatctgg ccggcctggc agtatccgca ctgcggcacg
    10381 ttgttcttga cccaggcttt ctgcagcgga tgatcgccgt tcctgctcag gccctcgatg
    10441 gtaaccacgg ggcccgacac cgaaccgacc ggcagtgcgc aggagcgcat cgccatgccg
    10501 ttgacgtgga ctgtacaggc gccgcactgc gccatgccgc agccgaattt tgttccggtc
    10561 agacccagcg tgtcccgaag tacccagagc aagggcatct cttcggggac gtccacctgc
    10621 tgcgccttcc cgttgacgtt cagggaaatt gccatgacct ctcctcccga ctgatgatga
    10681 aaaaacgcga cccaccgaaa gcccgaatcg cataccggcg cccatagttg ttgttgtggg
    10741 cctaagcact gcttacgact gccgccgaaa agatcttcgc acgcgtggcg gtcagcaaaa
    10801 gtagcccgcc tgaagatcag cccggcaaac ctgcgaaaac acacccaccg ggccatcgtg
    10861 tcccggcggc gcgccttcta gcagttgaag cgggaacggt gaagctgcaa cgcgaccggt
    10921 gcaaaccggc tggcaatccg cagctgcgat cgcggaaact tcggagagaa tgccggcatt
    10981 gcgcctggac agcgcaacgt aactcgccgg atgctttctc ctcccgggac gggcttcgag
    11041 accctttagt tcctgccgga ttgcgggatt cgattttgtt gcggccccgc ttcctgctat
    11101 ggaagccaaa gtaacacttt gccgcgggct tgaacagatt tgccacggga agattttccg
    11161 ccgcgttgcg ggagttggaa cggcaggtgc gtattgcgcc gcaataaaag actccccgcc
    11221 agccggcctt aggccggcaa taggagcgtc gacgctgagg gcgcaaagaa gggcaaggtt
    11281 gaaggcgcaa ggacgaagga acggcaagag gcggattctg cgtgccgagc gccgtgaaga
    11341 aaccagccgg ccgtgctcac ggccctacca gctcgctgat gaagttgcgc tcggcgagga
    11401 tgaccgagga gcggccggac ttgcccgatc gctccaggcg caacaatttg ctgctttggc
    11461 gccagacgag tacggcattt gatgaagttg tcccggtgtc ggctttcctg atttccacgg
    11521 tgccgggacc gtactcctgg gcgagcaatt ccgccacctg gtcgaaatgg ctctcctgga
    11581 aactggctgc gattccggaa agccgttcgc cggtgaaata cagcgtcagc cgatcgatcg
    11641 gcgtgccctt gaaggcaact gccccggtcg ccttgtaggc acaggccgaa aacagaaaac
    11701 aaccggccag gctttcgcag tcgtaggcgg gatcgttcag gatcgcatcg taaggcaggc
    11761 cgagcgtgtg tccgccgagc gagaacgact ccgccgcgcg ggcacccgcg gccgcgaggc
    11821 aaagccctcc ggcggcaagc aggctgatta ttcggctggc tttcatatcg ctcacattat
    11881 cagagtcggg gctcgggact cggcgcaaac cagccggccg atggccggcg acaagggcgt
    11941 tgacgcaaag gacgcagagg aagggcagga aaggacgcaa aggaattcag tgcgggaacg
    12001 agcgtgacat tctgtgccaa gtctgcgtga ctgctggtgg gagctcagca acgtcaattc
    12061 ctggttattc cttcgtgttc cttcactttt ccttcgtgat ccttcgtgtt gagtgcggtt
    12121 atatccggct agcggccggg cggcatcagt gcgagaaggt gccggtttcg atgcggtggc
    12181 gaaccatgct gatatgctcg cgcagcacgt agagctggtc ggcgaatgac agcggcgtct
    12241 tgatgttgtt gacatactct tcaatgttgt ccaggcgctt caggatctgc tcgcgccgct
    12301 ccggcgtgag ctcgccgagc atgtcgcgct cgatcgccat cagcgtgccg taccagcggt
    12361 agatgcgcga gcgcacccgc cagcgataca gtgggggaac gatgcgcgtc accgggatga
    12421 tcagcaccag catcggaacc agtacgacca ggagccgatc caccaggctc gccagccaga
    12481 acggcagctg cttgtagaga aactgctttc ccgatttgta gtagcgctcc gcgtccgggc
    12541 tgatcggaaa atcacgctcc agcggtgccg ggaattcgcc cgcgtcgcgg aacagacccg
    12601 gttcgccgtg cacttcccgc gccgcgctga tcagcaggtc cgagagtgcg ggatgcaggc
    12661 cttcgcgcgc caccagttcc accgtcggac cgatcagttg atagttcttc ggcggaaaat
    12721 tggcgcccag gtcgatcgag ccttccggca gggtaaggcg cgacagaaat cggaacttgc
    12781 gcacgtagcc gttggcctgc ttgaagctca tcaactcgat gccaggtacc tggcgcaacc
    12841 ggcgcattac cttgcctgtg gccgaatcgc cggtgaggaa ggcggcgtcc accgaaccag
    12901 cgatcagcgc gtctgcggct tcctctccgg ccaattcgag gagcacggta ggctcgccgt
    12961 caatcgcatt ggccttcaac agtttgagca ccagcgcccg ggtgccgctg ccttccgggc
    13021 cgatcgccag ccgcttgccc gctagctgcg ataaccgctc caccggctcg gggttgcgat
    13081 agtagaccat gacgggctgg ggaaacatgc tgcccagcga catcaggccg gtgatgttca
    13141 cgccatcggt cagccctccc tgcacgaagg cgacgtccag cttgaaagcc ggattggcca
    13201 accgctgcag gttttcaagc gagccgcggg actcgaggat ctgcactttc accccatggg
    13261 cctcgatgat cttgcggtat ttctccgcgg tggcgaggtt gctgccgccc ggagcgccgg
    13321 cgctgataat gatggtgttg ggcggagcgg ggcggacaaa cttgaccgcg atccaggcgg
    13381 ccgccgccgt gatcaggagc acgggcacgc cgattaccag tagatcgcgc caggacaccg
    13441 ccaccagtct tgccggaagc cgcatccgcc ggcgtctttc cacgttgcct tgttccactt
    13501 gccggttgcc ttccactcgg gacgccttcg aagcgccgct gccgtcattg ttcgccatga
    13561 tgaagtctcc gtttcaggct ttcagtatcg cggcgccgct ggtggcgagc accagaatgc
    13621 cgatcgccgc gatccagctt ccagccacac gcaccgcgat atgaatccag cccgctttcc
    13681 ggcgcagcag gtaatcggtg acgatcaatc cgtaagcggt caccgcaaga ccagcgagcg
    13741 caacgccggg aatatataga tagggcttgg tctgttcgat catgccggcg ccgttggcaa
    13801 ggccgtggct cagcccgaac acagcggcga tgctgcagca gaacgccatc ggcaatgacc
    13861 atgccgccgc taccagcccg ccgaacagga tggccgagag aatattgagc agtactacgt
    13921 acggcggcgg ggggatccat agcgcaacgg ttgcgcccgc cgcgagcgcc aggcagaaca
    13981 cgagcagtac cggctcggct ttccggcctt gctggcccgc gagaatcccg agcgccagga
    14041 acgggagtac gtgttccaat gcagtcagcg catgcaatgc gccggcataa aaatcaccgg
    14101 cgccagggtt cgccgaatgc gcgtatgcgc agcccgccgc gagcagcagg cacagtgcga
    14161 cgccggctct cacggccggc atcgaaaggc gggcaggcat gatgacttag tgtcctgttc
    14221 cgccaattcg cggctgaaaa cccgacgaga atcggcgcca tgctgtgtta cgagtcct
//
LOCUS       DASQOP010000318         5350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_970557, whole genome shotgun sequence.
ACCESSION   DASQOP010000318 DASQOP010000000
VERSION     DASQOP010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970557"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2388
                     /locus_tag="VE030_12525"
     CDS             <1..2388
                     /locus_tag="VE030_12525"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZF24278.1"
                     /translation="LVVSAALIVIALTLLIRPQIVSPQNSEAPRNAAPEPKTASVQFD
                     AQEREWLAAHPVIRVGAETSYAPYEFVDNRGKFAGVVADYLEIIKRRLGVEFQVFQLL
                     NFATVQDKLQRRDLDIVLAVTRAQERQPFLAFTKPYLHYVNVIVTRDDYVFVSGLRDF
                     APERLAVVQGHSSQSLMVRAYPNQLVPAYPDILEGLIAVSTGKVDGLVDDIFPIVFNI
                     RQRQISNLRIAAPVEKALQPQGFSVGVRNDWPELVTILDKVLDAISDEEKRAISEKWL
                     SVRYESKVDYRAIWTSLAVFSVILVAGVLYIRLLSAQRRALMAARAELEAASRAKDQF
                     LANISHELRTPLHAILGYAELVRERPLADPGRQEALATIARSGRHLLDLINDLLDLSR
                     IRSGRLELHLSPVSLPALLEEIAAMVRVDTERKGLEFNLEASGDLPAQVDADGKRLRQ
                     ILLNLLGNAIKFTNAGRVTLTVQTFPLGSERVELRVWVRDTGIGIAPEDTGRIFAPFE
                     QAEPGRKRESGVGLGLAISQELAHLMGGDIEVESRPNGGSTFRFAVALPLVKEPQAIL
                     PEHRRIIGYQGTSKCIMVADDQEENRQLLQQILEPLGFEVAAAAGGQTAVAAALQRRP
                     ALIVMDLRMPEMSGFQAAHSIRQTLELATVPIVAASASTADLERAEADSLTFAACLRK
                     PFESTELLDIIARLLDLTWRYEETETHGSTPMREQADTQLIAPSGATLDELLELARLG
                     KLVRVEQIALELERGEARYRPFGRRLYTLARDLDEERLITLLQDCAGTHRDVAPN"
     gene            2372..3493
                     /locus_tag="VE030_12530"
     CDS             2372..3493
                     /locus_tag="VE030_12530"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HZF24279.1"
                     /translation="MSLPTELRGATVLIVDDKPQNLRLISDFLAEQGFDLMLTRSGAQ
                     ALEKARLARPDLVLLDVRMPETDGFEVCRRMKADPRTAGVPVIFMTAVDDTAHKVEGF
                     RLGAVDYVTKPIQREELLARIQHHLQLSRLQKELTAKTQDLALKNAELEAYAHTIAHS
                     LKTPLAAAGRFLEILCKFKSDDLSGEQRHLALQAQSALAMTGEVVDALLLLSTVAQQH
                     VELKLLDTGSLVSRALSQLGEMLARQRADVKLPQAWPQALGYAPWVGEVWLNLLSNAL
                     KYGGSPPRLEVAGAPDGPHVRFSVRDNGQPLSEDERKRVFTPFTRLHKERAAGHGLGL
                     ATVQRIVSKLGGTVGVTVGSAGGNEFFFTLPAPPVRAPD"
     gene            3575..4198
                     /locus_tag="VE030_12535"
     CDS             3575..4198
                     /locus_tag="VE030_12535"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZF24280.1"
                     /translation="MKILHADDHPMFREGLRFFLQLLGTQVTALEAGNLRAALDKLAL
                     EWPVDLLLLDLQMPGMSEIEGFLAIRRAYPALPIVIVSGINDPQIIRTLLDGGARGYI
                     PKFSGSEQLMDALRRVLNGEVYVPDAMFLPPSQAAGNGESAPLTSRQLQILPLLAEGM
                     PNKRIADVLGLAEGTVKQHLKDLFRRLNASNRTQAVREARRLGLLPK"
     gene            complement(4266..4916)
                     /locus_tag="VE030_12540"
     CDS             complement(4266..4916)
                     /locus_tag="VE030_12540"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZF24281.1"
                     /translation="MTVSSRESTHLWSLSEKHLKALSERGTIKAYPKNTVIVNDGDRS
                     DTLFIILSGKVKAYLADEDGKEVLLSTQGPGEYFGEMILDQGPRSASVMTLEPSKFSV
                     ITTDQFKKFLTEHPDAGPELVMSLIHRVRELTKTVGNLALLDVYGRVARLLLELATEE
                     EGEMVISERLTQQDMANRVGCSREMISRILKDLRIGGYLRMDGERIIIARNPPRAW"
     gene            complement(5096..5314)
                     /locus_tag="VE030_12545"
     CDS             complement(5096..5314)
                     /locus_tag="VE030_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24282.1"
                     /translation="MTTTLNKLFIAATLFCFAVIACDKARVTTENGGHPVEFTQHGNT
                     ACVLVDNKVFCAPAKMRVPVRLVSSSAI"
BASE COUNT         1058 a         1642 c         1643 g         1007 t
ORIGIN      
        1 cttgtcgtgt cggcagcgtt aattgtcatc gcgctgacgc tcctcatacg cccacagatc
       61 gtgtcgcccc agaatagcga ggcgccgcgc aacgctgccc ccgagccgaa aacggccagc
      121 gtccaattcg acgcacaaga aagggagtgg ttggcagcgc atccggtcat aagggtcggc
      181 gcggaaacga gttatgcgcc ctacgagttc gtcgataacc ggggcaaatt tgctggagtc
      241 gtagccgact acctggaaat tatcaaacga cgactcgggg tggagtttca ggtctttcag
      301 ctgctgaatt tcgcgacagt tcaggacaag ctgcaaagga gggatctgga catcgtgctt
      361 gcggtcacgc gcgcgcagga acgccagccc ttccttgcct tcaccaaacc ctatctgcat
      421 tacgtaaacg tcatcgtcac ccgtgacgac tatgttttcg taagcggatt gcgtgatttc
      481 gcgcccgagc gtctggccgt cgtgcagggg cactcgtccc agtcgctgat ggtaagggca
      541 tatccgaacc agcttgtgcc tgcctatcct gacatcctcg agggcctgat agcggtgtcc
      601 acaggaaaag tggacggcct ggtggacgac atctttccca tcgtatttaa catcaggcaa
      661 cggcagatca gcaatctaag gattgccgcg ccggtcgaga aggctttgca gccgcagggc
      721 ttctcggtgg gtgtgcgcaa cgactggccg gagctggtca ccattctcga caaggtgctc
      781 gacgcgatca gcgacgagga aaagcgcgca atttcggaga aatggctgtc ggttcggtat
      841 gaaagcaaag tcgactaccg ggccatctgg acatcgctag ccgtgttctc cgtcatcctt
      901 gtggcgggcg tactgtatat ccgcctgctc agcgcgcagc gacgggctct gatggcagcg
      961 cgcgcggagc tcgaggcggc aagccgcgcc aaggaccaat tcctagccaa cataagccac
     1021 gagttgcgca cgccgctgca cgctattctc gggtatgcgg aacttgtgcg cgagcgcccg
     1081 ctggccgacc ccggccgcca ggaggcactg gccacgatag cgcgcagcgg ccgtcacctg
     1141 ctcgacctga tcaatgacct cctggatttg tcgagaatcc ggtccggccg cctcgaattg
     1201 cacctttctc ccgtttctct gccggcattg ctcgaggaga ttgccgcgat ggtgcgtgtg
     1261 gacaccgaga ggaaagggct ggagttcaat ctcgaagctt cgggcgacct tccggcgcag
     1321 gtggatgcgg acggcaagcg cctgcggcag atcctgctca acctgctcgg caacgccatc
     1381 aagttcacca acgccgggcg ggtgaccctg accgttcaga cgtttccgct cggcagtgag
     1441 cgcgtcgaac tccgggtctg ggtgcgcgat accggcatcg gcatcgcgcc ggaggatacc
     1501 ggccgcatct tcgcaccctt cgagcaggcg gagccggggc gcaagcgcga gtccggtgtc
     1561 ggcctcgggc tggccatcag tcaggagctt gcacacctga tgggcgggga catcgaggtc
     1621 gaaagccgcc cgaacggcgg cagtactttc cgtttcgcgg tggcgctgcc actggtcaag
     1681 gagccgcagg ctatcctgcc cgaacaccgg cgcatcatcg gctaccaggg aacgtccaaa
     1741 tgcatcatgg tcgccgacga tcaggaagag aaccgccagt tgcttcagca aatactcgaa
     1801 cccctgggat tcgaagtcgc cgccgccgca ggtggacaga cagcagttgc cgccgcgcta
     1861 cagcggcgtc cggccctgat cgtgatggat ctgcgcatgc cggaaatgtc cggcttccag
     1921 gctgcccact ccattcgcca gacgctcgaa ctggctacgg tgccgatagt ggcagcatcc
     1981 gccagcaccg cggacctcga acgggcagaa gcagattcgc tgactttcgc ggcctgcctg
     2041 cgcaagccgt tcgagagcac ggaactgctt gacatcatcg cgcgcctgct cgacctgacc
     2101 tggcgctacg aagagaccga gacccatggg agcacgccga tgcgggagca ggcagataca
     2161 cagctcatcg cgccttccgg tgccacgctc gacgaattgc ttgaactcgc gcgactgggc
     2221 aagctggtgc gcgtcgagca gatcgcgttg gagctggagc ggggcgaagc gcggtatcgc
     2281 cctttcggac gccgcctgta cacgctcgcc cgggatctcg acgaggagcg actcatcacg
     2341 ctgttgcagg actgcgcagg aacacatcgc gatgtcgctc ccaactgagc tgcgtggcgc
     2401 aaccgtgctg atcgtcgacg acaagccgca gaacttgcgc ctgatctcgg atttcctggc
     2461 tgagcaagga tttgacctga tgcttacccg cagtggggcg caggcgctcg aaaaagcacg
     2521 cttggccagg ccggatctgg tgcttctgga cgtgcgcatg ccggaaacgg acggcttcga
     2581 ggtatgccgg cgcatgaaag ccgatccgcg gaccgccgga gtcccggtga tcttcatgac
     2641 cgcagtggac gacaccgccc acaaagtcga gggttttcgc ctcggtgcgg tggactacgt
     2701 caccaaaccg attcagcgcg aggaactgct ggcgcgcatc cagcaccacc tgcagctgag
     2761 ccggctgcag aaggagttga ccgccaaaac acaggatctg gcgctgaaga atgccgagtt
     2821 ggaagcttat gcgcatacca tcgcacacag cctcaagacc ccgctcgcag ccgctggccg
     2881 cttcctggag attctttgca agttcaaatc ggacgatctg tccggcgaac agcggcatct
     2941 ggcgctgcag gcgcaaagcg cgctggccat gacaggagaa gttgtcgacg ccctgctcct
     3001 gctatccacg gtcgcacagc agcacgtcga acttaaactg ctcgacacag gcagtctcgt
     3061 cagccgggct ctgagccagt tgggtgagat gctggcgcgt cagcgcgccg acgtgaagtt
     3121 gccacaagcc tggccgcaag cgttgggcta cgcgccctgg gttggagagg tatggctgaa
     3181 cctgctcagc aacgcgctca agtatggcgg ctcgccgccg cgtctcgagg tcgcgggtgc
     3241 gccggatggc ccgcacgtgc gcttcagtgt gcgggacaac ggccagccgc tttcggagga
     3301 cgagcgcaaa cgcgtgttta cgccattcac tcgcctgcac aaggagcggg ccgcaggtca
     3361 cggcttaggc ctcgccacgg tgcagcgcat cgtgtcgaag ctcggaggca cggtgggtgt
     3421 gaccgtcggg tcggctgggg gcaacgagtt ctttttcacg ttgccggcgc cgccggtacg
     3481 cgcgccggac taaaatccgg accgcgatcc tgcgttcgac ggtaacacga atgccagccc
     3541 gcacttttgc aactcaaacc gctgcaatgc cgccatgaag atcctgcacg ccgacgacca
     3601 tcccatgttc agggaaggat tgcggttctt cctgcagctt ctggggaccc aggtgacggc
     3661 gctggaggcg ggcaacttgc gggcggcgct ggacaagctt gccctggagt ggcctgtgga
     3721 tctgctgctg ctcgacctgc agatgccggg aatgagcgag atcgagggct ttctcgccat
     3781 tcgccgggcc taccctgcac tgccgatagt aatcgtctcc gggataaacg atccgcagat
     3841 catccgtacc ctcctcgacg gcggtgcgcg cggatacatt ccgaaattta gcggcagcga
     3901 gcaattgatg gacgcattgc gtcgcgtatt gaacggggaa gtctacgtgc ccgatgcgat
     3961 gttcctgccc ccctctcaag ccgcgggcaa cggcgaatcg gcgccgctga cctccaggca
     4021 gttgcagatt cttcctttgc ttgccgaagg catgcccaac aaacgcatcg cggacgtact
     4081 ggggctggct gagggaaccg tcaagcagca tctgaaggat ctgttcaggc gcctcaatgc
     4141 cagcaatcgc actcaagctg tcagggaggc caggcgcctc gggctgctgc caaaataggc
     4201 cgcaggaacc cgctctactg tcgggacgac aatgcgaaat cttcgaacca ggcccgacgg
     4261 ccggtttacc acgcacgcgg agggttcctg gcgatgatga tccgttcgcc gtccatcctc
     4321 agatagccgc cgatgcgcag gtccttgagg atgcggctga tcatttcgcg cgaacacccg
     4381 acgcggttcg ccatgtcctg ctgggtcaag cgctcgctga tgaccatttc gccctcttcc
     4441 tcggtagcca gttccagcaa tagcctggca acgcgcccat agacatcgag cagcgcgaga
     4501 ttgccgaccg tctttgtcag ttcgcgcacg cggtggatca ggctcatgac cagttccggt
     4561 cctgcatccg gatgttcggt cagaaatttc ttgaactgat cggtcgtaat aaccgagaac
     4621 ttcgagggtt cgagcgtcat caccgacgcg gagcgagggc cctggtcgag gatcatctcg
     4681 ccgaagtact cgcccggccc ctgggtgctc agcaatactt ccttgccgtc ctcgtcagcg
     4741 agatacgcct tcacctttcc cgacaggatg atgaaaagcg tatcgctgcg atcgccgtcg
     4801 ttgacgatga ccgtattctt tggataagcc ttgatcgtac cccgctccga gagtgccttc
     4861 agatgcttct ccgacaaact ccagagatgc gtgctctctc tcgatgatac cgtcattgaa
     4921 actccccttg gtcctgattg ggccattgcg gcgaaaagtc tgcgctcaac acgctgccat
     4981 gacaaggcac cttgggggag cttcacaggg tgcaccggat tggctttgga tgcgaaacga
     5041 acaaagaaaa ggcccgcttc gccggcgggc ctaaagctag aggagaactg gagattcaaa
     5101 tggcgctaga ggaaaccagc cgcaccggca cgcgcatctt tgcgggcgcg cagaaaacct
     5161 tgttgtcgac caatacgcaa gcggtgttgc catgctgggt gaattccacc gggtggccac
     5221 cattctcggt cgtgacccga gctttatcgc aagctattac ggcaaagcag aacagcgtgg
     5281 ccgcgatgaa cagcttgttc agagtcgtag tcataatctg ggtcctcctt gaagcatttc
     5341 atagtgggac
//
LOCUS       DASQOP010000319        18074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2585332, whole genome shotgun sequence.
ACCESSION   DASQOP010000319 DASQOP010000000
VERSION     DASQOP010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 18074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18074
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2585332"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="VE030_12550"
     CDS             complement(<1..831)
                     /locus_tag="VE030_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic amidase"
                     /protein_id="HZF24283.1"
                     /translation="MLHGDISSSKDTVGVAVVNYKMPRLHTKKEVLENARKIAEMIKG
                     MKVGLPGMDLVIFPEYSTHGIMYDSKEMYDTASSCPGEETEIFAGACRAAKVWGVFSL
                     TGERHEEHPNKAPYNTLILMNDKGEIVQKYRKIMPWVPIEGWYPGNCTYVSEGPKGLK
                     VSLIICDDGNYPEIWRDCAMKGAELIVRCQGYMYPAKEQQVIMAKAMAWANNVYVAVA
                     NAAGFDGVYSYFGHSAIIGFDGRTLGECGEEDYGIQYAALSKGLIRDARKNMQSENHL
                     F"
     gene            complement(963..1652)
                     /gene="urtE"
                     /locus_tag="VE030_12555"
     CDS             complement(963..1652)
                     /gene="urtE"
                     /locus_tag="VE030_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HZF24284.1"
                     /translation="MLTVTGLNVFYGESHVVRDVSFDLRDGETVAIMGRNGMGKSTLL
                     KSLIGILRPRGGKIILNDRDITNAETFDRVAAGMGYVPQGRMIFPYLTVEENILAGME
                     TSVSKEIPAYVYSVFPVLEEMRMRRAGNLSGGQQQQLAIARALVSNPKVLVLDEPTEG
                     IQPSIIKDIARALNKLKADRKFALLVSEQVLGFAKEIADRFLIIERGHFVYEGFRDNL
                     DTKSIHAYLTI"
     gene            complement(1669..2406)
                     /gene="urtD"
                     /locus_tag="VE030_12560"
     CDS             complement(1669..2406)
                     /gene="urtD"
                     /locus_tag="VE030_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HZF24285.1"
                     /translation="MSELILSIENLSVSFDGFKVVDDLVFYVDKDELRCVIGPNGAGK
                     TTLLDMICGKTKPSNGKIRFKNYELTQMIEYQITRVGVGRKFQNPSVYEDLTVFENLE
                     ISYPKKRGILGSLFFKRDEELIRKIDKVASQIFLHEVLRHKAAQLSHGEKQWLEIGML
                     LMQDPELLLLDEPVAGMSPREREQTAELLNGISKGRSIVVIEHDMAFVRMIAHKVTVL
                     HQGRLLAEGSMDEVQGDERVKEVYLGH"
     gene            complement(2403..3578)
                     /gene="urtC"
                     /locus_tag="VE030_12565"
     CDS             complement(2403..3578)
                     /gene="urtC"
                     /locus_tag="VE030_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HZF24286.1"
                     /translation="MTDGVPFERAGEAAAADRHDRGIWIGRAGLAILALLLFAVLPLA
                     LDVFRLGLAAKYLSLAFCAVGIVLIWGYGGILSLGQGVFFGLGSYMMAMFLKLESTSS
                     GSSAQALSSFFGSNLPDFMVWNSVETLPWWWEPFHYVWFTIPAILLLPPLLAFGFGYA
                     IFRKRVGGVYFSIITLSLSAIMSIMIIGQQGVTGGVNGINDFRTFMGIDLNTDEAKRT
                     MYFITCALLLMCVVAGSFILKSRLGKVLVAIRDREDRVRFSGYDPALFKAFIFAIAAF
                     FSSLGGAMFTIQVGLASPSLVSIVPSVEMVIYAAVGGRLSLIGAVYGAVLVGAAKTLF
                     SENFVEYWMYFIGGLFVFVVMFLPHGLAGLLERIRGYRTILPKRFKAPEIERGGLGK"
     gene            complement(3578..4480)
                     /gene="urtB"
                     /locus_tag="VE030_12570"
     CDS             complement(3578..4480)
                     /gene="urtB"
                     /locus_tag="VE030_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HZF24287.1"
                     /translation="MTLDMFAMQAFTGLSIFTILMLMALGLAIVFGLMGVINMAHGEL
                     MAMGAYTSYGTAYFFQHFLPGWMDAYIFVGIALAFVVTSIFGYLLERGLIRFLYQRPL
                     DTLLATWGVSLILQQCYRQFINPREVEVPTVSWLRGAWQVTEGVSLPLSRIFIICLAI
                     VTAVLVYLLLYRTRSGILIRAVTQNRSMAGAAGINTKHVDAYTFALGCGLAGIAGSSF
                     TMLASTGPTTGQQYIVDTFIVVVFGGVESLIGTAASAFIIGQLQSWFEFGMSGSMARA
                     SILVLVIVVLYFRPNGLFATKVRR"
     gene            complement(4612..5802)
                     /gene="urtA"
                     /locus_tag="VE030_12575"
     CDS             complement(4612..5802)
                     /gene="urtA"
                     /locus_tag="VE030_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HZF24288.1"
                     /translation="MSKFDRRDFLKAGAATAAAGILPAPFIWPRGAQAADTVKVGILH
                     SLTGTIAIAEKSVVDAEMLAIEEINNAGGVMGHKIEAIVEDGASDWPTFAEKARKLIV
                     RDKVASVMGCYTSASRKAVLPVFEKDKGLLYYPTYYEGLEISPNIMYTAQEATQSVIA
                     AMEWLYKNKGKSFYMIGSDYIWPRTTIKIAKATLARLGGKLVGEGYYPLGHIEFSSEI
                     NKIKASKPDVVLNCVVGGSNVAFFKQLTAAGITGKNQTVLSLAVSEEEVSGIGSDNTE
                     GTLTCMGYFQSLKNSANEKFVKAFKAKYGQNRVVGDTLECGYIAVYLWKMAAEKAKSF
                     DVEKVVAASSELPMDAPEGKVKFHKSNHHLWKHARIGTFRKDGQVDMIYESSLIEPNP
                     FPKL"
     gene            6453..7610
                     /locus_tag="VE030_12580"
     CDS             6453..7610
                     /locus_tag="VE030_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZF24289.1"
                     /translation="MKSRSTAPIRVGVLFSETGVTSVIERSERQGTLLAVQEINEAGG
                     INGRELQLISYDPQSRPALYASQAKHLLLEDRVQVILGCYMSSTRKAVMPVVERWNGL
                     LLYPTLYEGFEYSRNVIYTGAAPNQNSVQLAEFMMREFGSRVFMVGSDYIYPYESNRI
                     MSDLIVERGGEKVAEVYLPLDASWESYVTVAKKIQATNPDFIFSTVVGYGTSLLHRAY
                     AQVKLDPAKMPIASLTTCEAEVRQMGADIAEGHITSAPYFQSVDTETNRRCVAQFKNR
                     FGADEVTNMCWEAAYFQTHLLADAMRRVDTDNIVDLLRVLPGSEFSGPQGKVRIDEHN
                     HHTYLRPRIGRVNGQGQFDILQEAERWVRPDPYLVSHSLQDWSTRAQIHAR"
     gene            7633..8247
                     /locus_tag="VE030_12585"
     CDS             7633..8247
                     /locus_tag="VE030_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HZF24290.1"
                     /translation="MRKSEGTPRLLRELRSLRVLVFHPDDQDGQELLGQLQRIGCRVK
                     AFWPPLDKLPDETDLVIFAMRPEVLSMDLPWLRRDGSPPVIPVVNYENPVIVEAVLQL
                     NAYGVIASPVKSFGLLTSIVVSVNQAEKARAQEKYIARLEQRLAGLRKINKAKAILMS
                     TRGLSEDDAYKVIRDQAMSKRVTAEEIADAVINANDILGFDLKS"
     gene            complement(8268..9974)
                     /gene="ureC"
                     /locus_tag="VE030_12590"
     CDS             complement(8268..9974)
                     /gene="ureC"
                     /locus_tag="VE030_12590"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006758739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HZF24291.1"
                     /translation="MTFKLTRAAYADMFGPTVGDRVRLADTELWIEIEKDHTIYGEEV
                     KFGGGKVIRDGMGQSQRPHAEVADTVITNALIVDYSGIVKADIGIKNGRISGIGKAGN
                     PDIQPGVNIVVGAATEIIAGEGMITTAGGVDAHIHFICPQQIEEALMSGITTMLGGGT
                     GPATGTNATTCTPGPWHIARMLQAAEAFPMNLGFLGKGNCSLKDPLIEQVAAGAMGLK
                     LHEDWGTTPAAIDNCLAVADDMDIQVAIHTDTLNESGFVDTTIAAFRGRTIHTYHSEG
                     AGGGHAPDIIRVCGEANVLPSSTNPTRPFTVNTLDEHLDMLMVCHHLDPGIPEDVAFA
                     ESRIRKETIAAEDILHDLGAISMMSSDSQAMGRVGEVVMRTWQTAHKMRVQRGLLPEE
                     GSDCDNFRVKRYIAKYTINPAITHGIAHEVGSLEVGKLADIVLWRPAFFGVKPGLIIK
                     GGMIAAAAMGDPNASIPTPQPVHYRPMFGSFGPACQATSLTFVSAAGIENGIGNALGL
                     RKRLVAVRGTRTLGKKDLPLNGYAPKIEVNPETYEVRADGEHLTCGPATSLPMAQRYF
                     LF"
     gene            complement(9971..10279)
                     /locus_tag="VE030_12595"
     CDS             complement(9971..10279)
                     /locus_tag="VE030_12595"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HZF24292.1"
                     /translation="MIPGEIRTQPGEIELNAGRKTVTVTVINSGDRPVQVGSHYHFFE
                     TNEALQFDRKSAYGYRLNIPAGTAMRFEPGQNSRVELVELGGGRTVYGFLGKVMGALK
                     "
     gene            complement(10309..10611)
                     /gene="ureA"
                     /locus_tag="VE030_12600"
     CDS             complement(10309..10611)
                     /gene="ureA"
                     /locus_tag="VE030_12600"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HZF24293.1"
                     /translation="MDLAPREKDKLLIFTAALLAERRKARGLKLNYPEAVAFISAGVL
                     EGARDGKSVSELMSYGRTLLGRNEVMEGVPEMIPDIQVEATFPDGTKLVTVHNPIS"
     gene            complement(10822..11991)
                     /locus_tag="VE030_12605"
     CDS             complement(10822..11991)
                     /locus_tag="VE030_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZF24294.1"
                     /translation="MVESISGAIPRIGLILTGGGARAAYQVGFLRAVSHMLPRGTPNP
                     FQIICGTSAGAVNAAVMAAGATDFHRAVRRLMLVWKYFHADQVYRSDVLSVISTGSKW
                     LGAMLLGGLGRNNPTSLLDNSPLAQLLRRYLDFNGIRSSIEAGALYALSVTASGYTSG
                     QSVSFFQGVPGLAGWQRARRIGVPEMIDIDHLMASSALPFIFPAVPLRREFFGDGSMR
                     QIAPISPALHLGADRVIVIGAGRHAGAATERTRTIAYPSLAQIAGHALNSIFLDSLEV
                     DIERLQRINRTIRLISPEDRSRHDVQLREIDVLVISPSEEIDRIAARYTRELPRPIRF
                     LLRGLGATRTGGATLTSYLLFEPAYCGALIALGYKDTMAKREEILRFIGPAVTQK"
     gene            complement(12035..12313)
                     /locus_tag="VE030_12610"
     CDS             complement(12035..12313)
                     /locus_tag="VE030_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24295.1"
                     /translation="MTSDHHAQEIQMLRSEIEMLMNERQSLLRATGAAAVFVANLDSE
                     SLPESTYAVADILAHALNQLSEDSLRDALELVRPTVEAARIEAKKQES"
     gene            complement(12464..12694)
                     /locus_tag="VE030_12615"
     CDS             complement(12464..12694)
                     /locus_tag="VE030_12615"
                     /inference="COORDINATES: protein motif:HMM:NF044585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24296.1"
                     /translation="MAGENKTKVTILTGTYRIKGHIELTVGARVTDYLVESKEFIAVT
                     DAEVWEIAGRHVMNTPFLNVSRAHIEIVIPGH"
     gene            12847..13980
                     /locus_tag="VE030_12620"
     CDS             12847..13980
                     /locus_tag="VE030_12620"
                     /inference="COORDINATES: protein motif:HMM:NF042665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6607 family protein"
                     /protein_id="HZF24297.1"
                     /translation="MLRQISFLILLALSPLAAQAHEPGEADGANASWFTFAWPLGENT
                     LKPRGASTRGAAVVLDPAPGEAWRRLREPGLSTLERDRRAILAMAGPYRVSFNFLEVL
                     RFDPALKPDAPYQSWGTEYVFVSEDRENFIALQHILVMRVKMEDGSTGMPMVVRHWRQ
                     EWRYQSGALLAYEGANTWARRPVPAQQRDGAWVQSVFQVDDSPRYAARGRWQHNDSNS
                     TWVSDETWRPLPRREWSVRKDYQVLVGTNRHTITADGWVQEENNLKLALDDNGNARAQ
                     MPYLAREYGFARYQRIKDYDFSAGENYFARTEPFWAEVRSAWRELAQREARFTLKAPV
                     DQGRLFEPFFDYAQTLADSKRFDRDDARAFIDRTLRDSYLARD"
     gene            14205..15746
                     /locus_tag="VE030_12625"
     CDS             14205..15746
                     /locus_tag="VE030_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine permease"
                     /protein_id="HZF24298.1"
                     /translation="MSAAGSAASPRWWVPGDWNGFFGLFTNVLLNVIVLTTLCLYVVQ
                     LPPDTVFGRVLPAMGIALPIGNLFYAYLAWQLAKKEGRSDVTAMPYGPSVPHMFIVVL
                     VVMLPTYLATKDAMLAYAAGLAWAFIIGVIVLLGCFVGPWIRKMTPRAAMLGTLAGIS
                     IAFISMRPAFQMWEVPWIGFVCFAIVLIAWTANIRLPGGIPGGLAAVIVGAAIGWLAA
                     AFGWSDYMKGDQVGKAFEQFGLHLPWFSADVFRGLAGIAPLLATAIPLGIYNFTEAMN
                     NVESASAAGDNYNLRKILAADGIGAIVGSMLGSPFPPAVYIGHPGWKSVGGRIGYSLA
                     TGVAIALVCFLGLTALLLNLIPLVAILPILLYIGLVIGAQAFQASPSKHAPAVVLAII
                     PNIAEWAKINIDGALGAAGTSADKVGMAALGQVGVVYQGLAVLGSGSVLAGMVLGAMA
                     AFIIDRKLSHAAVTAFIGAALSFIGLIHGPALGWMVSPLVSLGYVMFGALCLAMSRQT
                     AAAPS"
     gene            complement(15967..16920)
                     /locus_tag="VE030_12630"
     CDS             complement(15967..16920)
                     /locus_tag="VE030_12630"
                     /inference="COORDINATES: protein motif:HMM:NF009007.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HZF24299.1"
                     /translation="MAKRKHTAVVAVGGNSLIVDDQHQSIPDQVKAATTTSHYVADMV
                     EAGWTAVLTHGNGPQVGFILRRSGIAKGVVPEVPVDYADADTQGAIGYMFQRALYNEF
                     QRRKLKRKVVAVVTQVLVDPNDPAFRNPTKPIGAHMTEDVAKSLANDFGWVVREDAGR
                     GWRRVVASPMPQQIIERDIIEALIDLGYIVIACGGGGIPVLRDAQGDLQGLEAVIDKD
                     FASSLLARDLGAELFVVSTGVEKVAVDFGKPTQRWLERVSLAEVRKYLAEGQFPKGSM
                     GPKIEAIISFLEGGGTRALITNPPNLGRALSGATGTHIVTD"
     gene            complement(16932..>18074)
                     /locus_tag="VE030_12635"
     CDS             complement(16932..>18074)
                     /locus_tag="VE030_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HZF24300.1"
                     /translation="PIAWEEMCGPMQGAVIGAILYEGWAADADAAWRLAGSGSIAFEP
                     AHHHAAVGPMAGVISPSMPVWIVENAAGRNRAFSNLNEGLGKVLRFGANSPEVLKRLH
                     WMAQVLAPALAAALAKLGSMELKPLIAQALQMGDEVHNRNAAASSLFLKKIVPAALHS
                     GFDAQAIADGVTFIAGNDHFFLNLSMAACKTMLDAAHGVPGSSLVTAMSRNGVRFGIR
                     LSGTGEQWFEAPSPVVGGLYFPSYGPEHAAPDMGDSSITETAGLGGFAMATAPAIVQF
                     VGGTPQDAVANTREMGHITLGRNSAFTLPAMNFSGTPAGIDARRVLDTNITPIINTGI
                     AHKEAGVGQIGAGITRAPLECFTQAIIALASRAGVGMPGSIRSTLS"
BASE COUNT         3446 a         5729 c         5323 g         3576 t
ORIGIN      
        1 gaacagatgg ttctcggact gcatgttctt gcgtgcgtcg cggataagac ctttggaaag
       61 cgcggcgtac tggatgccgt aatcctcctc gccgcactcc ccgagggtgc gtccgtcgaa
      121 gccgatgatc gccgaatggc caaagtagga gtaaacgccg tcgaagccag cggcattcgc
      181 caccgccaca tagacgttat tggcccaggc catggccttg gccatgatca cctgctgctc
      241 cttggccggg tacatgtagc cctggcagcg cacgatcagc tccgcgccct tcatcgcaca
      301 gtcgcgccag atctccggat agttgccgtc gtcgcagatg atcagcgaca ccttcagtcc
      361 cttcggaccc tcggaaacgt aggtgcaatt gcccggatac cagccttcga tcggaaccca
      421 gggcatgatc ttgcgatact tctgcacgat ctcgcctttg tcgttcatca ggatcagcgt
      481 gttgtagggc gccttgttcg ggtgctcctc atgcctctct ccggtgagcg agaacacgcc
      541 ccagaccttg gccgcgcggc aggcgccagc gaagatctcg gtttcctcac ccggacagct
      601 cgaagcggtg tcgtacatct ccttgctgtc gtacatgatc ccgtgggtgc tgtactcggg
      661 aaagatcacc aggtccatgc cgggcaggcc gactttcatg cccttgatca tttccgcgat
      721 tttcctggcg ttttccagca cttccttttt ggtatggagg cgcggcatct tgtaattgac
      781 aacggcgacc cctacggtgt ctttactgct cgaaatgtct ccgtgtagca ttttccttct
      841 cctttacctc gctcgttaat cacatgctcg cccttgagct gccctctggt cgccgccggc
      901 attcagacca gcaatgccgg atgcgaaaaa gatcttgctg ttgcagatcg gtcccgacgt
      961 cttcagatcg tcaaatacgc gtgaatgctt tttgtatcca ggttgtccct gaatccctcg
     1021 tacacgaagt gccctctctc gatgatcagg aagcggtccg cgatttcctt cgcgaaaccc
     1081 agcacctgct cggacaccag caatgcgaac ttgcggtccg ccttgagctt gttcagcgcc
     1141 ctcgctatat ccttgatgat ggacggctga atgccctcgg tcggctcgtc gagaaccagc
     1201 accttgggat tgcttacgag tgcacgcgca atcgccagtt gctgctgttg tcctcccgaa
     1261 aggttccctg cgcgtcgcat gcgcatttcc tccaaaacgg gaaacacgga atacacgtac
     1321 gcgggaattt ccttgctcac cgaagtttcc attcccgcca ggatgttttc ctccaccgta
     1381 aggtagggaa agatcatgcg cccttgcggg acgtagccca ttccggcagc cacccggtcg
     1441 aaagtctccg cattggtaat atcccggtcg ttcaggatga tcttcccgcc tcgggggcgc
     1501 aagatcccga tcagcgactt gagcaatgta ctcttgccca tcccgttgcg gcccatgatc
     1561 gcaacggtct ctccgtcccg cagatcgaag gatacgtctc tcactacgtg gctctctccg
     1621 tagaagacgt ttaacccggt tacggtaagc acctctaccc ctctccaatc agtggcccaa
     1681 gtaaacttcc ttgacccttt cgtcgccctg cacttcgtcc atgctgcctt ccgccagaag
     1741 acggccctgg tgcaaaaccg tgaccttgtg cgcgatcatg cgcacgaagg ccatgtcatg
     1801 ctcgatcacg actatagacc tgcccttcga gatcccgttc agaagctccg ccgtttgttc
     1861 cctttccctg gggctcatcc ccgccaccgg ttcgtcaagc agcaggagtt cggggtcctg
     1921 catcagcagc atcccgattt ccagccattg cttctctcca tggctgagct gcgcggcctt
     1981 gtggcgcagc acttcgtgaa ggaaaatctg gctggcgacc ttatcgatct tgcgaatcag
     2041 ctcctcgtcg cgcttgaaga acagcgagcc gagaataccg cgctttttcg ggtatgaaat
     2101 ttccaggttc tcgaacacgg taagatcttc gtacacggac gggttctgaa atttcctgcc
     2161 gacgccgacc cgcgtaatct gatattcgat catttgcgtc agctcgtagt tcttgaaccg
     2221 gatttttccg ttggatggct tggtctttcc gcagatcatg tcgagcagcg tggtcttgcc
     2281 cgcaccgttt ggaccaatca cacatctcaa ttcgtccttg tcgacataaa acaccagatc
     2341 gtccacgacc ttgaatccgt caaacgagac gctcaaattc tcaatcgaca ggatcagttc
     2401 gctcattttc cgagcccgcc gcgttctatt tcaggcgcct tgaaccgctt gggcaggata
     2461 gtccggtaac cgcgaattcg ctccagcaat cctgccagcc catggggcag gaacatcacc
     2521 acgaaaacga acagtccgcc gatgaaatac atccagtatt caacgaaatt ctcggagaac
     2581 aaggtcttgg ccgcgcccac aagcaccgcg ccgtacacgg caccgatgag ggacagccgg
     2641 ccgccgacgg ccgcatagat caccatctcg accgatggca cgatgctcac cagcgaaggc
     2701 gaagccaggc cgacctggat cgtaaacatc gcgccgccaa gcgaggagaa aaatgccgca
     2761 atggcaaaaa tgaatgcctt gaacagcgcg gggtcatacc cggagaatct cacgcggtct
     2821 tcgcggtccc tgatcgcgac cagaaccttg cccagccggc ttttcagaat aaaactcccg
     2881 gcgacaacgc acatgagcag aagcgcacaa gtgatgaaat acatcgtccg cttcgcctcg
     2941 tccgtattca ggtcgatccc catgaaagta cggaaatcgt ttatgccgtt gaccccgccg
     3001 gtaacgccct gctgaccgat aatcatgatg gacatgattg cggacagtga cagcgtgata
     3061 attgaaaagt acacaccgcc gactcgtttg cgaaaaatcg cgtagccgaa accgaacgcc
     3121 agcaaaggcg gcagcagcag gatggccgga atggtgaacc agacgtagtg aaagggttcc
     3181 caccaccatg gcagcgtttc gacgctgttc cagaccatga aatcgggaag gttggatccg
     3241 aaaaaggagg acaaagcctg cgccgaactg cctgaagagg tcgactccag cttgaggaac
     3301 atggccatca tgtaacttcc caggccgaag aacactccct gcccgagact caggattccg
     3361 ccataacccc atatcagaac gattcctacg gcacagaacg ccaggctgag atactttgcg
     3421 gccaatccca gacggaaaac atccagcgcg agaggcagca ccgcgaacaa taacaaggca
     3481 agaatcgcca gcccggctct tcctatccaa atcccgcggt cgtgccgatc cgcggcggca
     3541 gcctcgcccg cacgctcgaa cgggacgcca tcggtcatca tctcctcacc ttcgttgcga
     3601 acagaccgtt gggccggaaa tacagaacca cgattaccag aaccagaatg ctcgcgcgtg
     3661 ccattgaccc gctcatcccg aattcgaacc aggactgcaa ctggccgatg ataaacgccg
     3721 aggcagccgt tccgatgagg ctctccacgc cgccgaatac gacgacaatg aacgtgtcga
     3781 cgatgtactg ctgacccgtt gtcggcccgg tcgaggccag catcgtaaag gaactgcccg
     3841 cgatacctgc aaggccacag cccaaggcaa atgtgtacgc atcgacgtgc ttggtattga
     3901 tcccggcagc accggccatc gaacggttct gcgtcacggc tcgaatcagg atccccgaac
     3961 gcgtacggta caggagcagg taaaccagta cggccgtgac gattgccagg cagatgatga
     4021 aaatccgcga tagcggcagc gaaacgcctt cagtcacctg ccacgcgccc cgaagccagg
     4081 aaacggtagg cacttcgacc tctcgcggat tgatgaactg gcggtagcac tgctggagaa
     4141 tgagactgac gccccaggtc gccagcagcg tgtcgagcgg acgctgatac agaaaccgga
     4201 tcagtccgcg ctccaacaga tatccgaaga tactggtgac gacgaacgcg agcgcgatgc
     4261 ccacgaagat gtaggcgtcc atccacccgg gcaggaaatg ctggaaaaaa taagcggtac
     4321 cgtaactggt gtacgccccc attgccatca attcgccatg cgccatgttg atgacgccca
     4381 tcaggccgaa gacaatggcg agccccaggg ccatgagcat gaggatggta aagatgctca
     4441 gtccggtgaa ggcctgcatg gcgaacatat ccagagtcat atgacggtcc gcccctgttt
     4501 caaccgatca actccatgca tcccaagacg gcgagccgcg gagaacgccg cggaaagatg
     4561 ctttccgcgg cccctccgcg cccgcgtcca ggtgcaaaac gccaactgcc gttacagctt
     4621 cgggaaagga ttcggctcga tcaatgaaga ttcgtaaatc atgtccacct ggccatcctt
     4681 gcggaaagtg ccgatgcgtg catgcttcca caaatggtga ttggacttgt ggaatttgac
     4741 cttcccctcc ggcgcatcca tcggcaattc ggaagatgcc gccaccactt tctccacgtc
     4801 gaaactcttg gctttctcgg ccgccatctt ccacaggtag accgcaatat agccgcactc
     4861 cagggtatcc ccgacgacgc ggttctgtcc gtactttgcc ttgaacgcct tgacgaactt
     4921 ttcgttcgcc gaattcttca ggctctggaa atagcccatg caggtcagag tcccttcggt
     4981 attgtcggaa ccgatcccgg acacctcttc ctccgacacc gccagcgaca gaaccgtctg
     5041 gttctttccg gtgatgccgg cagcagtcag ttgcttgaag aaggcgacgt tgctgccccc
     5101 cacaacgcag ttgaggacga cgtcgggctt ggaggccttg atcttgttga tctcggaaga
     5161 gaactcgatg tgtcccagcg ggtagtagcc ctcgccgacc agcttgccgc cgagacgcgc
     5221 cagcgtggcc ttggcgatct tgatcgtagt ccgcggccag atgtaatccg agccgatcat
     5281 gtagaaggat ttgcccttgt tcttgtacag ccattccatg gcagcgatca ccgactgggt
     5341 cgcttcctgg gcggtgtaca tgatgtttgg cgatatctcg agaccttcgt aataggtcgg
     5401 gtagtacagg agtcccttgt ccttctcgaa taccggcagc acggctttgc gggatgccga
     5461 cgtgtagcag cccatcaccg acgccacctt gtccctcacg atcaacttgc gggccttctc
     5521 cgcaaacgtc ggccaatcgc tggcgccgtc ctcgacgatc gcctcgatct tgtggcccat
     5581 gacgccgccg gcattgttga tctcctcgat cgccagcatt tccgcgtcga caactgattt
     5641 ttccgcgatc gctatcgtcc cggtaagcga atgcaagatg ccaaccttca cggtgtcggc
     5701 cgcctgcgca cccctgggcc agataaaggg tgccggcaaa atacccgccg ccgccgtagc
     5761 ggcgccagcc ttcaaaaagt cccgcctgtc gaacttgctc attgtgattc ctctcccgtc
     5821 ggaaacgtgc gccgcaagtc gcggcaacca gaaaaaaagc ccactggccg atacctccgg
     5881 ccagtgggct cctctgcctc gctcggcttc gacgtctttg tcttcagccg aaaaaactca
     5941 acgctttcgc ggacacgctt ccgtgcgctt ccgcattttc gctgaccgtg acgcaatatc
     6001 cgggccaact cgattcgcaa acctatcagt cggccattgg tcaccgcgcg atcacgaccg
     6061 ggcgggccgg gtaacgccgg cctggcagac ggctttcaga agaaattggg acaggcctct
     6121 tgccctgctc gtgttgtgcg cttccgggtt tcgcgctgcg gtaatccttg tgtctgcact
     6181 gagttcgtgc cacttcgcaa gtccggcgcc catttgtggg tcatccccaa tcccgcgccg
     6241 agagtgacgg ccctccccgc ttcgggcata tttataatcc ctacgcgtgc caaattcaag
     6301 cgctgattgc tcttcgcacg gcgttcatca tggcctacac ttctgcacac gctcaagcct
     6361 gaattcgggc tttcggcaat atcctccgcg cccgggcaag ggcgcggagg tacagaaacg
     6421 ggaattaccc cagtaacagt ccaggcagga aaatgaaatc cagatcaacg gcaccgatcc
     6481 gtgtcggcgt cctgttttcc gaaaccggcg tcacctcggt tatcgagcgc tccgaaaggc
     6541 agggtacgct gctggcggtg caggaaatca acgaagccgg cggaatcaac ggccgcgaat
     6601 tgcaattgat cagttacgat ccgcaatcac gcccggcgct gtacgcatcg caggcaaagc
     6661 atttgcttct ggaggaccgg gtccaggtca ttcttggctg ctacatgtcg agcacccgca
     6721 aggcggtgat gccggtcgtc gagcgctgga acggcctgct tctgtatccg acgctctatg
     6781 agggtttcga gtactcgcgc aacgtcattt ataccggcgc cgccccgaac cagaacagcg
     6841 tgcagctcgc ggagttcatg atgcgcgaat tcggctcccg ggtattcatg gtcggatcgg
     6901 actacatata tccgtacgaa tccaaccgca tcatgagcga cctgatcgtc gaacgcggcg
     6961 gcgagaaggt agcggaggtc tacttgccgc ttgacgcatc ctgggaatcc tacgtcaccg
     7021 tcgcaaaaaa gatccaggca acgaacccgg atttcatttt ttcgacagtt gtagggtacg
     7081 gcacatcttt gctccatcgg gcatacgcac aggtcaaact ggacccggcg aaaatgccca
     7141 tagcgagcct cactacttgc gaagccgaag tgcggcagat gggagccgac attgcggaag
     7201 gtcacatcac ttccgcaccc tactttcaga gcgtcgatac cgaaaccaac aggcgctgtg
     7261 ttgcccaatt caagaatcgt tttggcgcgg atgaagtaac caatatgtgc tgggaagcgg
     7321 cgtacttcca gactcacctg ctcgccgatg caatgcgccg cgtcgatacc gacaacatcg
     7381 tggacctgct ccgggtcctt ccgggctcgg aattttccgg gccgcagggc aaagtgcgga
     7441 tcgacgagca taaccaccac acctacctgc ggccgcgcat cggcagggtg aatggccaag
     7501 gccagttcga catccttcag gaagcggagc gctgggttcg acccgatccc tacctggtgt
     7561 cgcactcgct ccaggactgg tcaacccgcg cgcagattca cgcccggtaa tcgctgcccc
     7621 ggcggaaatc cgatgagaaa gagcgaaggc acgccgcgtc tgctgagaga gttgcgctct
     7681 cttcgcgtgc ttgttttcca tcccgacgac caggacgggc aggaattgct cggtcaactg
     7741 cagcgcatcg gttgccgcgt gaaggcattc tggcctccgc tggacaagct ccccgacgaa
     7801 acggatctgg taattttcgc catgcggccc gaggtgctgt caatggatct gccctggctg
     7861 cggcgcgatg gatctccgcc ggtcattccc gtggtgaact acgaaaatcc ggtgatcgtc
     7921 gaagcggtgc tgcagcttaa tgcgtacggc gtgattgcct ctccggtcaa atctttcggc
     7981 ctgctcacct cgatcgtggt atccgtgaac caggccgaga aagcccgggc gcaggaaaaa
     8041 tacatcgcgc gtctcgagca aaggcttgcc ggactgcgca agataaacaa ggccaaggca
     8101 atcctgatga gtacccgcgg cctgtcggaa gacgacgcct acaaggtcat ccgtgatcag
     8161 gcgatgagca aacgcgtcac cgccgaagaa atcgccgacg ccgtgatcaa tgcgaacgat
     8221 atactcggtt tcgatctgaa gtcataaggg cgcgcgcggg cgcgccatca gaacaggaaa
     8281 tagcgctgcg ccatcggcag ggacgtggcg ggcccgcagg taagatgttc gccgtccgcg
     8341 cgaacttcgt aggtttccgg gttcacctcg atttttggcg cgtaaccgtt cagcggcagg
     8401 tctttctttc cgagggtccg cgttccgcga accgcgacca accgctttcg cagaccgagc
     8461 gcgttgccga ttccgttctc gatgcctgcc gccgatacga aagtcagcga cgtcgcctgg
     8521 caggccggcc cgaacgaacc gaacatcggc cggtagtgga ccggctgcgg cgtcgggatg
     8581 gacgcgttcg ggtcgcccat cgcggccgcg gcgatcatgc cgcccttgat gatgagaccc
     8641 ggtttgacgc cgaagaaagc cggcctccag agtacgatgt cggcaagctt gccgacctcc
     8701 agcgaaccta cttcgtgcgc gatcccgtgg gtgattgccg ggttgatggt gtacttggcg
     8761 atgtagcgct tgacccggaa gttgtcgcag tcggatccct cctccggcag caggccgcgc
     8821 tgcacgcgca tcttgtgcgc agtctgccag gtgcgcatca ccacttcgcc cactcttccc
     8881 atggcctgcg aatcggacga catcatgctg atcgcgccta ggtcatgcag gatgtcctcc
     8941 gcggcgatgg tctccttgcg gatgcgcgat tccgcgaacg ccacgtcttc gggaatgccg
     9001 gggtcgaggt gatgacacac catgagcatg tcgaggtgct cgtccagcgt attgacagtg
     9061 aaggggcgcg tcggattggt cgacgaagga aggacattgg cctcgccgca cacgcggatg
     9121 atatccggcg cgtggccgcc gcccgcgccc tccgagtggt aggtgtgaat ggttcttccg
     9181 cggaaggccg cgattgtggt atcgacaaac cccgactcgt tgagagtgtc ggtgtggatc
     9241 gccacctgaa tgtccatgtc gtctgccacc gcgaggcagt tgtcgatcgc tgccggcgtg
     9301 gtgccccagt cctcgtgcag cttcaggccc atcgcgccgg ccgccacctg ctcgatgagc
     9361 ggatccttga ggctgcagtt gcccttgccc aggaaaccca gattcatcgg aaacgcttcc
     9421 gcggcctgga gcatgcgcgc gatatgccac ggtcccggcg tgcaggtcgt cgcattggtt
     9481 ccggtcgccg gcccggtgcc gccgccgagc atcgtggtga tgccggacat caacgcctcc
     9541 tcgatttgct gcgggcagat gaagtgaatg tgcgcgtcca caccgccggc ggtggtgatc
     9601 atgccttcgc cggcgatgat ctcggtggcg gcgcccacaa cgatgttcac accgggctga
     9661 atgtcggggt tgccggcctt gccgatcccg gagattcttc cgttcttgat gccgatgtcg
     9721 gccttgacga ttcccgagta atccacgatg agcgcattcg ttatcacggt atcggcgacc
     9781 tccgcgtgcg gacgctggct ctgtcccatg ccgtcgcgga tcaccttgcc accgccgaat
     9841 ttcacttcct caccatagat ggtgtggtcc ttttcgatct cgatccacaa ttcggtatcg
     9901 gcaagtctca cgcggtcgcc gacggtcgga ccgaacatgt cggcatacgc cgccctggtc
     9961 agtttgaaag tcatttcagc gcgcccatta cttttccgag aaaaccgtag acggtacgcc
    10021 cgccgccgag ttctaccaac tcgacgcggc tgttctgacc cggctcgaag cgcatcgcgg
    10081 tccccgcagg aatgttgagc cgataaccgt aggcgctctt gcggtcgaac tgcaatgcct
    10141 cattggtttc gaaaaaatgg tagtgcgatc cgacctgaac cggccggtcg ccgctgttga
    10201 tcaccgtgac cgtgacggtc ttgcgtccgg cgttgagttc gatctcgcca ggctgtgtcc
    10261 tgatctctcc cggaatcatc gttgttctct cccgaacgtc ggccggcatc aagaaatcgg
    10321 attgtggacg gtaaccagct tggtcccgtc cgggaaagtc gcctcgacct gaatgtcggg
    10381 aatcatctcc ggcacgccct ccatgacctc gttgcgtccg agcaaggtcc tgccgtaact
    10441 catcagttcg gagacgctct tgccgtcgcg cgcgccttcg agaaccccgg cggaaatgaa
    10501 ggcaacggct tcggggtagt tcagcttcaa tccgcgcgcc ttgcgccgct ctgccagcag
    10561 tgccgcggtg aaaatcagca gcttgtcttt ctccctgggt gccaaatcca ttctcttgat
    10621 cctcttcgct taagtacgtt ttcgcggcct acacgccgtt gccactgcac agcccggccg
    10681 cggatcgttc gatgccgctc ggtcagtgaa gactcgacta acatggcaag gcgtcaagat
    10741 gtcaaagttt gcagcaaagc tcaagtgcct ggcatcggag caagaatcgt gcgactgcag
    10801 ggttattcac cagcgcctgg ttcacttttg cgtcaccgcc gggccgatga agcgcaggat
    10861 ctcctcccgc ttcgccatcg tgtccttgta ccccagcgca atcagcgcgc cgcaataggc
    10921 cggttcgaac agaaggtaac tggtcagcgt cgcgccgccg gtcctggtcg cgcccagacc
    10981 gcgcaaaaga aaacggattg gacgcggcag ttcgcgcgta taacgcgcgg cgatcctgtc
    11041 tatttcctcg ctcggggaaa tgacgagcac gtcaatttcg cgcagctgca cgtcatgcct
    11101 ggatcggtcc tccggagaaa tcagccggat ggtccggttg atgcgctgca ggcgctcgat
    11161 gtccacttcg aggctgtcga gaaaaatgct gttgagcgcg tggccggcga tctgtgccag
    11221 cgacggatag gcgatcgtcc tggtccgttc cgtcgcagca cctgcatgcc ggccggcacc
    11281 gatgacaatg acccggtccg cgccgaggtg cagcgccggg ctgatgggcg cgatctggcg
    11341 catactgccg tcgccgaaaa actcgcgacg cagcggcacg gccgggaaaa tgaacggcag
    11401 cgcactggag gccatgagat ggtcgatgtc gatcatttcc ggcacgccga tccggcgcgc
    11461 ccgttgccag cctgcaagtc cgggcacgcc ctggaagaag ctcaccgact ggcccgaggt
    11521 gtatcccgat gccgtaaccg acagcgcgta cagtgcgccg gcctcgatgc tgctgcgaat
    11581 gccgttaaag tccagatagc ggcgcagaag ttgcgcaagc ggggagttgt ccagcaacga
    11641 agtcggattg ttcctgccca gaccgccgag cagcatcgcc ccaagccact tgcttccggt
    11701 actgatgacg ctcagaacgt cggaccgata gacctggtcg gcgtgaaaat acttccagac
    11761 cagcatcagc cggcgcactg cgcggtggaa atcggtggcg ccggcagcca tcaccgccgc
    11821 gttgaccgcg cctgcggaag tcccgcagat gatctgaaac ggattgggcg taccgcgcgg
    11881 caacatgtgc gacaccgcgc gcaggaatcc gacctggtag gcagctcgcg cgccgccacc
    11941 ggtaaggatc agtccgatcc tgggtatcgc gccggatatg gattcgacca tggggatgcg
    12001 gccggccggc tgccgctacg tgacgctcct gccgtcagct ttcctgcttc ttcgcttcga
    12061 ttcgggccgc ttccaccgtc gggcgcacca gttcgagcgc atcgcgcagt gaatcctcgg
    12121 agagctggtt gagcgcatgg gcgaggatat ccgcaaccgc gtaggtgctc tcgggcagag
    12181 actcgctgtc gagattggca acaaacactg ctgccgcacc ggttgcgcgc aacaggcttt
    12241 gccgctcgtt catgagcatt tcgatctcgg agcgcagcat ctggatttcc tgcgcgtggt
    12301 ggtcgctggt catggcactc tccctgtgtc cttaacccca tgatgccaca agacttttag
    12361 gagagccaga aatcgctgcg caatctacga atatctgatg cagattgcgg tcgtgctttg
    12421 gcagcagcgc aagcgccgtt gaaatcaacc gggaggcgct ggctcaatgt cccgggatta
    12481 cgatctcgat atgagcgcgg ctcacattga gaaacggcgt gttcattaca tgccggcccg
    12541 cgatctccca gacctccgca tcggtgacgg caatgaattc cttcgactcg accaggtagt
    12601 cggtcacccg cgcacccacc gtcaattcga tatgcccctt gatccggtag gttccggtca
    12661 ggatggtcac tttggtcttg ttctcgccag ccataaacac cctcctgggt cagtctgcaa
    12721 ttttcggtct cggcaacgga attgagcctc gaatatttac gcaagtatgc gcgtttggca
    12781 acgccggctg ttgcccgtcc tcattcgtct acaatctgcc tgtcttcggg atattcgagt
    12841 atctccatgc tgcgacaaat atcgttcctc attcttctcg cgttgtcgcc ccttgccgcg
    12901 caagcccatg aacccggcga ggcggacgga gccaacgcga gctggttcac tttcgcgtgg
    12961 ccgctgggcg agaacacgct gaagcctcgc ggtgccagca cgcgtggcgc cgcggtcgtt
    13021 ctcgaccccg cgcccggcga agcctggagg cggttgcgcg agcccggcct gagcacgctg
    13081 gagcgcgacc gccgcgccat cctggccatg gctggcccgt atcgtgtcag tttcaatttc
    13141 cttgaggtac tgcgattcga tccggcgttg aagcccgacg cgccctatca gagttgggga
    13201 acggaatacg tcttcgtatc ggaggaccgc gagaatttca tcgccttgca gcacatcctg
    13261 gtgatgcgag taaaaatgga agacggcagc accggcatgc cgatggtagt gcgccactgg
    13321 cgccaggaat ggcgttacca gtccggcgcg ctgctggcct acgaaggtgc caacacctgg
    13381 gcgcggcgcc cggtgcccgc gcagcagcgc gacggcgcct gggtgcagag cgtgttccag
    13441 gtcgatgatt ccccgcgcta cgcggcgcgc ggacgctggc agcacaacga cagcaactcc
    13501 acctgggtca gcgacgaaac ctggcgtccg ctgccgcgcc gcgagtggag cgtgcgcaag
    13561 gactatcagg tattggttgg aaccaaccgc cacaccatca cggcggacgg ctgggtgcag
    13621 gaggagaaca acctcaagct cgcgctcgac gacaacggca atgcgcgtgc ccagatgccg
    13681 tacctggcgc gcgaatacgg attcgcgcgc taccagcgca tcaaggacta tgacttcagc
    13741 gcgggagaaa actacttcgc gcgcaccgag ccgttctggg ccgaagtgcg gtccgcctgg
    13801 cgcgaacttg cacagcgcga ggcacgcttc acgctcaagg cgccggtaga ccagggccgc
    13861 ctgttcgagc cattcttcga ctatgcgcag acccttgccg acagcaagcg cttcgatcgc
    13921 gacgatgcgc gcgccttcat cgaccgcacg cttcgcgaca gctatctcgc gagggactga
    13981 gaaaaaccgc gattcgagcg tcgtgcggtc atcccggccg cagccgcgat gcggctgatc
    14041 gagccgggaa ggtctggact tgccggtctt gcgggcgcat gcttttgcct gtccggcgac
    14101 gggccgttgg cctatgacga cgccatcatt gcggccgata agttgcctgc gcagtgctgc
    14161 cgggggaaga ctgcaaataa atccaacacc gaaggggaga aaccatgagt gctgccggat
    14221 ctgctgcaag cccgcgctgg tgggtgccgg gcgattggaa cgggttcttc ggactcttca
    14281 ccaacgtcct gctcaatgtc atcgtcctga ccacgctgtg cctgtacgtg gtacagcttc
    14341 cgcctgacac ggtcttcggg cgcgtgctgc cggcgatggg catcgcgctg ccgatcggca
    14401 acctgttcta cgcctatctg gcatggcaac tggcgaagaa ggagggccgc tccgacgtca
    14461 cggcgatgcc ttacgggccg agcgttccgc acatgttcat cgtggtgctg gtggtcatgt
    14521 tgcccaccta tctcgccacc aaggacgcga tgctcgccta tgcggcgggg ctggcgtggg
    14581 ccttcatcat cggcgtgatc gtgctgctgg gctgcttcgt cgggccgtgg atccgcaaga
    14641 tgacaccgcg cgcggcgatg ctcggcacgc tggccggcat ttccatcgcc ttcatctcga
    14701 tgcgcccggc gttccagatg tgggaagtgc cgtggatcgg cttcgtctgt tttgccatcg
    14761 tgctgatcgc ctggaccgcg aacatccgcc tgcccggcgg gattcccggc ggcctcgcgg
    14821 cggtgattgt cggcgctgcg atcggctggc tcgcggccgc gttcggctgg agcgattaca
    14881 tgaagggcga ccaggtcggc aaggccttcg agcaattcgg cctgcacctg ccgtggttct
    14941 cggcagacgt gttccgcggc ctggcgggaa tcgcgccgct gctcgctacc gccattccgc
    15001 tgggaatcta caacttcacc gaggcgatga acaacgtgga gagtgcctcg gcggcagggg
    15061 acaactacaa cttgcgcaag atcctggccg cagacggaat cggggcgatc gtgggttcga
    15121 tgctgggaag cccgttcccg ccggcggtgt acatcggcca tccgggctgg aaatcggtag
    15181 gcggccgcat cggctattcg ctcgccaccg gtgtggcgat tgcgctggtg tgtttcctcg
    15241 gcctcaccgc gctgctgctc aatttgattc cgctggtggc gatcctgccg atcctgctgt
    15301 acatcggcct ggtgatcggt gcgcaggcgt ttcaggcgag cccttccaaa catgcgccgg
    15361 cggtggtgct ggcgatcatt ccgaacatcg ccgagtgggc aaagatcaat atcgatggcg
    15421 cgctgggcgc tgccggcacc agcgccgaca aagtaggcat ggccgcgctc ggccaagtcg
    15481 gtgtggtcta ccagggcctg gcggtgctcg gcagcggatc ggtgctggcg ggcatggtgc
    15541 tcggggcgat ggcggcgttc atcatcgacc gcaagctcag ccacgccgca gtgacggcat
    15601 tcatcggcgc ggcactgtcc ttcatcggcc tgatacacgg gccggctctg ggctggatgg
    15661 tttcgccgct ggtgtcgctc ggttacgtga tgttcggcgc gctgtgcctg gcgatgtcgc
    15721 gccagactgc ggcagcaccg agttgacggg cgacaggacg cggggccacg cggctgtctc
    15781 atgaggccgc acccatatcc tcgcgcgggt gcccaggttt cggcgcacgg tcaacacacg
    15841 actgcgcgcc gaaacttacc cgctgtgcga ggacttcggc caaatcgacg accacgcagc
    15901 cccgcgtccc gggcgcgtgc cggtgccgtg ctggaagaag tctgttgaaa ctttgtggga
    15961 agcgcttcag tcagtaacga tgtgcgtgcc tgtggcgccg gataatgcgc gccccagatt
    16021 gggcggattg gtgatcagcg ctcgcgtgcc gccgccctcc agaaatgaaa tgatcgcctc
    16081 gatcttcggg cccatgctcc ctttcgggaa ctggccttcg gcaagatatt tgcgcacctc
    16141 agcgagcgat acgcgttcga gccagcgctg cgtcggcttg ccgaagtcga ccgccacttt
    16201 ctccacgcca gtggaaacca cgaacagttc ggcgccgagg tcgcgcgcaa gtaggctgga
    16261 agcgaagtcc ttgtcgatca ctgcctccag tccctgcagg tcgccctgcg cgtcgcgcag
    16321 caccggaatg ccgccgccac cgcaggcgat cacgatgtag cccaggtcga tcagcgcctc
    16381 gatgatgtcg cgctcgatga tctgttgcgg catcggcgag gccaccacgc gccgccaacc
    16441 ccggccggcg tcttccctca ccacccagcc gaaatcgttc gccagcgact tggccacgtc
    16501 ctcggtcatg tgcgcgccga tcggcttggt cggattgcgg aacgctggat cgttcggatc
    16561 caccagcacc tgggtcacca cggccaccac cttgcgcttg agcttgcggc gctggaactc
    16621 gttatagagc gcgcgctgaa acatgtagcc gatcgcgccc tgcgtgtcgg cgtcggcata
    16681 gtccaccggc acctcgggca ccacgccctt cgcgattccc gagcggcgca ggatgaaacc
    16741 cacctgcggg ccgttgccgt gcgtgaggac cgcagtccag ccagcctcga ccatgtcggc
    16801 gacgtaatgc gacgtcgtag tcgccgcctt cacctggtcg ggaatcgact ggtgctgatc
    16861 gtccacgatc agcgaattgc cgccgacagc aacgaccgcc gtgtgcttgc gcttggccat
    16921 cgccatctcc tctacgagag cgtggagcgg atgctgccag gcatgccgac gccggcccgc
    16981 gacgccagcg cgatgatggc ctgggtgaag cactcgagcg gcgcgcgcgt aatgccggcg
    17041 ccgatctggc cgaccccggc ttccttgtgc gcaatgccgg tgtttatgat cggcgtgatg
    17101 ttggtgtcaa gcacgcggcg ggcgtcgatg ccggcgggcg tgccgctgaa attcatcgcc
    17161 ggcagcgtga atgcgctgtt gcgtcccagc gtgatgtgcc ccatttcgcg cgtgttagcg
    17221 accgcgtcct gcggggtgcc gccgacgaac tggacgatgg cgggcgctgt cgccatggcg
    17281 aagccgccaa ggccagcggt ctcggtgatc gagctgtcgc ccatgtccgg tgccgcgtgc
    17341 tccggcccgt agctcgggaa gtacagtccg cccaccaccg gggacggtgc ctcgaaccat
    17401 tgttcgccgg tcccggaaag gcggatgccg aagcgcacgc cgttgcgcga catcgcggtg
    17461 accaggctgc tgcccggcac gccgtgcgcg gcgtcgagca tggtcttgca cgccgccatc
    17521 gacaagttca ggaaaaagtg gtcgttgccg gcaatgaacg tcacgccatc ggcgatcgct
    17581 tgcgcgtcga agccggagtg cagcgccgcg ggcacgatct tcttgaggaa caacgacgac
    17641 gcggccgcgt tgcggttgtg gacttcgtcg cccatctgca gcgcctgcgc tatcaacggc
    17701 ttgagttcca tcgatcccag cttcgcaagc gccgcggcga gcgccggcgc aagcacttgc
    17761 gccatccagt gcaggcgctt gagcacttcc ggagaattgg caccgaagcg cagcaccttg
    17821 ccgaggccct cgttcaggtt ggagaacgcg cggttcctgc ccgccgcatt ctcgacgatc
    17881 cataccggca tcgacgggct gatgaccccg gccatcggcc ccaccgcggc gtgatgatgc
    17941 gcgggctcga aagcgatgct gccgctgccg gccagccgcc atgccgcgtc tgcgtccgcc
    18001 gcccagcctt cgtaaaggat ggcgccgatc accgcaccct gcatcggccc gcacatttcc
    18061 tcccaggcga tcgg
//
LOCUS       DASQOP010000320         3577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_367295, whole genome shotgun sequence.
ACCESSION   DASQOP010000320 DASQOP010000000
VERSION     DASQOP010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367295"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1680
                     /locus_tag="VE030_12640"
     CDS             <1..1680
                     /locus_tag="VE030_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6537 domain-containing protein"
                     /protein_id="HZF24301.1"
                     /translation="GVSVMIYDQTCAAEKRRRRKKGEYPKATQRVFINEAVCEGCGDC
                     GVQSNCTSILPVETEFGRKRAIDQSSCNQDYSCVKGFCPSFVTVEGGTPRKSRAARRE
                     NAAPLAPLSEPVSATLGETPYNILITGIGGTGVITIGALLGMAAHLEGKGTSALDMTG
                     MSQKNGAVTSHVRIAHTPAGIRAQRIATGEADLILGCDMLTAGAHDAISKTRPGRTVA
                     VINMHQQPPGQFAQNPNWQFPAEEIRALIDESVGGRAHFIDATRLATALLGDSIATNL
                     FMLGFAFQNGLIPLQRASILRAIELNGVAVDANKQAFDWGRRAALDLRQVETIATPAQ
                     PVVVQMPQNLETLLRRRIAFLTGYQDAAYAKRYEDLVAKVRDAETGIGAGDALSKAVA
                     KYLFKLMAYKDEYEVARLYASEDFEKRLNDSFEGEFTVKYNLAPPLLAKRDAQGHLVK
                     AQYGAWMKYAFKWLAKFKGLRGSFLDPFGRTAERRMERGLIREYEDLAEEIAGALSKE
                     NHGVAVALASLPERIRGFGHVKQRNVGAVAAEREALLARFRALAAGVEHAA"
     gene            1857..>3577
                     /locus_tag="VE030_12645"
     CDS             1857..>3577
                     /locus_tag="VE030_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633623.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HZF24302.1"
                     /translation="MTDLSVQAADQDKQAKNKVRFVTAASLFDGHDASINIMRRILQA
                     SGVEVIHLGHNRSVDEIVTAAIQEDVQGIAVSSYQGGHVEYFKYAIDLLRERGGAGIK
                     VFGGGGGVIVPSEIEELHSYGVARIYSPEDGARMGLQGMITDMIRTADRNLSAELPAS
                     LDEVFHGNVRDLARVITGLENATLPEPLRQKLAKRAAEHGSVPVLGITGTGGSGKSSL
                     TDELVRRLRIDQDDALKIAVIAVDPSRRKSGGALLGDRIRMNAINHPNIYMRSLATRD
                     AGAEIAACLPDVIAVCKCTGFDLILVETSGIGQGDAAIVPHVDFSLYVMTPEFGAASQ
                     LEKIDMLDFADFVAINKFDRRGAQDALRDVRKQMQRNRGAFNAATEAMPVFGTIASRF
                     NDDGVTALYHAIVAELKTRGLKAGDGRLKPTTVKASSGQNVIVPPARVRYLAEIAETV
                     RGYHADVTKQTRIARERQQLLEAKRMLDAAKKPSSDLEPLITERDKALSAEAKALLRE
                     WPLIRQSYSGDEQVTKIRDREMRTALTTTSLSGTKVPKVALPKFEDDGETLKWLMREN
                     LPGYFPYT"
BASE COUNT          709 a         1147 c         1162 g          559 t
ORIGIN      
        1 ggcgtttccg tgatgatcta cgaccagacc tgtgcggcgg aaaaacgtcg ccgccgcaaa
       61 aaaggcgagt acccgaaggc cacgcagcgg gtattcatca acgaagccgt ctgcgaaggc
      121 tgcggcgact gcggcgtgca atcgaactgc acttcgatcc tgccagtgga aaccgagttc
      181 gggcgcaagc gcgcgatcga ccagtcctcc tgcaaccagg actattcctg cgtcaagggc
      241 ttctgtccga gtttcgtgac cgtggaaggc ggcaccccga ggaagtcgcg ggcggcaaga
      301 cgcgaaaacg ctgcaccgct cgcgccgctg tccgaaccgg tctcggcaac gctcggcgaa
      361 acaccctaca acatcctgat cacgggaatc ggcggcaccg gtgtcatcac catcggtgcg
      421 ctgctgggaa tggccgcgca cctggagggc aagggcactt cggcgctgga catgaccgga
      481 atgtcgcaga agaacggcgc cgtcacttcg cacgttcgca tcgcgcacac accggcgggc
      541 atccgcgcgc aacgcatcgc caccggcgag gccgacctga tcctcggctg cgacatgctg
      601 accgcgggcg cgcacgatgc catttccaag acccggcccg ggcgcacggt agcggtgatc
      661 aacatgcacc agcagccacc cggccagttc gcgcagaatc ccaactggca gttcccggcc
      721 gaggaaatcc gcgcgctgat cgacgaatcg gtcggtggcc gcgcccattt catcgacgcc
      781 acccggctcg ccaccgccct gctgggcgac tcgatcgcca ccaacctgtt catgctcggc
      841 ttcgcgttcc agaacgggct gattcccctg cagagggcct cgatcctgcg tgcgatcgag
      901 ttgaacggtg tggctgtcga cgccaacaag caggcgttcg actggggacg ccgcgctgcg
      961 ctcgatctgc ggcaagtcga gacgattgcc acacccgccc agccggtggt ggtgcagatg
     1021 ccacagaacc tggagaccct gctgcgccgg cgcatcgcct tcctgaccgg gtaccaggac
     1081 gcggcctatg cgaagcgtta cgaggacctg gtcgcaaagg tgcgcgatgc cgagacgggc
     1141 atcggtgcag gggatgcgtt gagcaaggcg gtggcgaaat acctcttcaa gctgatggcg
     1201 tacaaggacg agtatgaagt cgcccggctt tacgccagcg aagatttcga aaaacggctt
     1261 aacgacagct tcgagggtga gttcacggtg aagtacaacc tggcgccgcc gctgctggcg
     1321 aagcgcgatg cgcaggggca tctagtgaag gcgcaatacg gtgcctggat gaaatacgca
     1381 ttcaagtggc tggcgaagtt caagggcctg cgcggcagct tcctcgatcc gttcggccgt
     1441 accgcggaga gaaggatgga gcgcgggctg atccgcgaat acgaagatct ggcggaagaa
     1501 atcgcgggcg ccttgtcaaa ggagaaccat ggagtcgccg tcgcgctggc cagcctgccg
     1561 gaacgcatcc gcggtttcgg ccatgtgaag cagcgcaacg tcggggcggt cgctgcggaa
     1621 cgcgaagcct tgctggcccg cttccgtgcc ctggcagccg gggtcgaaca tgccgcctga
     1681 cgcgccgcag cgcgatttgg gcaaggggtt atgctggtgg tcaacgggcc ggtggaccag
     1741 gtttacgttc acgtaaacgt aagcaataat ggctgccccg gcgcgatgac ccattccggc
     1801 atcagcgcgg aaacgcctgg gccgcgtcaa caaccgcaaa cggacgggac tgcttcatga
     1861 ccgatctcag cgtacaggcc gccgatcaag acaagcaggc gaagaacaag gtgcgcttcg
     1921 tcaccgccgc cagcctgttc gacggtcacg acgcctcgat caacatcatg cgccgcatcc
     1981 tgcaggcgag cggcgtcgaa gtcatacacc tcggccacaa ccggtcggtc gacgagatcg
     2041 tcaccgcggc catccaggaa gacgtgcagg gcatcgccgt atcctcgtat cagggtggtc
     2101 acgtcgaata tttcaagtac gcgatcgatc tgttgcgtga gcgcggcggc gcgggcatca
     2161 aggttttcgg cggcggcggc ggcgtgatcg ttccgtccga gatcgaggag ctacatagct
     2221 acggtgtggc gcggatttac tcgccggagg atggcgcgcg catgggcctg cagggaatga
     2281 tcacggacat gatccgcacc gcggaccgca atctttccgc ggagttgccg gcgagcctcg
     2341 acgaagtctt ccatggcaac gtgcgcgacc tggcgcgtgt gatcaccggg ctcgagaacg
     2401 cgacgctgcc ggagccgctg cgccagaagc tggcgaagcg ggcggcggag cacggcagcg
     2461 tgccggtgct cggcatcacc ggaaccggcg gctcgggcaa gtcgtcgctc accgacgaac
     2521 tggtccgccg cttgcgcatc gaccaggacg acgcgctcaa aatagccgtg atcgccgtgg
     2581 acccgtcgcg gcgcaagtcc ggcggcgcgc tcctgggcga ccgcatccgc atgaacgcga
     2641 tcaatcatcc gaacatctac atgcgctcgc tcgccacccg cgatgccgga gccgagatcg
     2701 ccgcctgcct gccggacgtg atcgccgtct gcaagtgcac cggcttcgac ctgatcctgg
     2761 tggagacttc cggcatcggc cagggagacg cggctatcgt cccgcatgtg gacttctcgc
     2821 tgtatgtgat gacacccgag ttcggcgccg ccagccagct cgagaaaatc gacatgctcg
     2881 acttcgccga cttcgtcgcg atcaacaagt tcgaccgccg cggcgcgcag gacgcgttgc
     2941 gcgacgtgcg caagcagatg cagcgcaatc gcggcgcctt caacgcagcc accgaagcga
     3001 tgccggtgtt cggcaccatt gcctcgcgtt tcaacgacga cggcgtgacc gcgctctatc
     3061 atgccatcgt cgccgagctc aaaacccgcg ggctcaaggc cggcgatggc aggctcaagc
     3121 cgaccacggt aaaagcgtct tcgggccaga acgtcatcgt tccgccggcg cgcgtgcgct
     3181 atctggcgga aattgccgaa accgtgcgcg gctatcacgc cgatgtgacg aagcagacca
     3241 ggatcgcgcg cgagcggcag cagttgctcg aagccaaacg catgctggac gccgcgaaga
     3301 aaccttcctc cgacctcgaa ccgctgatca cggagcgcga caaggcgctc agcgccgagg
     3361 caaaggcgct gctgcgggaa tggccgctga tccggcagtc ctattcgggc gacgaacagg
     3421 tcacaaagat ccgtgatcgc gagatgcgca cggcgctgac caccacgtca ctgtccggaa
     3481 ccaaggtgcc gaaagtggcg ctaccgaagt tcgaggacga tggcgagacg ctgaagtggc
     3541 tgatgcggga gaacctgccc ggctatttcc cgtacac
//
LOCUS       DASQOP010000321         2294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1777094, whole genome shotgun sequence.
ACCESSION   DASQOP010000321 DASQOP010000000
VERSION     DASQOP010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1777094"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VE030_12650"
     CDS             <1..626
                     /locus_tag="VE030_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZF24303.1"
                     /translation="GQVDPSSPWFGHPSFQIKYDLAEAKRLVAEAGYSPQKPLKTTFV
                     IAQGGTGQMLSLPMNEFLQQSFKEIGIDVDFKVVELETLYTHWRKGAADEMNAGITSN
                     NIAYVTSDPLYAIVRFFHSGQIAPVGVNWGGYKNPKVDALIDEAKQTFDTAKQDELLA
                     QAHSLIVDDAVLVWVVHDTNPHALSPKVKKFVQAQHWFQDLTQIGLE"
     gene            complement(953..1996)
                     /locus_tag="VE030_12655"
     CDS             complement(953..1996)
                     /locus_tag="VE030_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_044551230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HZF24304.1"
                     /translation="MNQIIRIGMDTSKHFFQLHGVDAAEQPVLRRKLRRKEMVAFFAK
                     LAPTVIGMEACGASHYWARELRKLGHEVKLIAPQHVKPYIKRNKNDGRDAEGLCEAMS
                     RPTMRFVPVKTEEQQAALMLAGLREQMVARRTQLSNMIRGYAAEFGITAAKGLDKIEP
                     LLADIAASQTLPVLARDLFVMHGREYAQLQAELKKIEARLMAWHRSNADSQRLAQIPG
                     IGPIGATALVMKAPDPRLFPSGRQFAAWIGLTPKDHSTAGKTRLGTITRAGDERLRSV
                     LVAGATAVIQQASRGRGRASPWLLALLARKSSPKLAAIALANKIARIAWKLLVTGASY
                     HAGGKPTASAHAA"
BASE COUNT          410 a          738 c          699 g          447 t
ORIGIN      
        1 tcggccaagt cgatccttcg agcccgtggt tcggccatcc gagtttccag atcaagtacg
       61 acctcgccga ggcgaagcgc ctggtcgcgg aggccggcta ttcgccgcag aagccgctca
      121 agaccacgtt cgtgatcgcg caagggggca ccggtcagat gctctcgctg ccgatgaacg
      181 aattcctcca gcaaagcttc aaggagatcg gcatcgacgt cgacttcaag gtcgtcgagc
      241 tcgagacatt gtatacgcac tggcgcaagg gcgcggcgga cgaaatgaac gccggcatca
      301 cgtccaataa tatcgcctat gtcacgtccg atccgctcta cgccatcgtg cgcttcttcc
      361 attccggcca gatcgcgccg gtcggcgtca actggggcgg ctacaagaat ccgaaggtcg
      421 acgcgctgat cgacgaagcc aagcagacct ttgacaccgc caagcaggac gaactgctag
      481 cgcaggcgca ttcgctgatt gtcgacgatg cggtgctggt atgggtggtg cacgacacca
      541 acccgcacgc gctgtcgccc aaggtgaaga agttcgtgca ggcgcagcac tggttccagg
      601 atctgacgca gatcgggttg gagtgagacc tcgatgtcgt cccggccaag cgagcgtagc
      661 gagcgctgag ccgggaccca tatgtggacg gcccccgtgc cgcaagagtt ttttcgaggt
      721 ctgatcggat cgcttgcatc catatgtccg gcctgttgat gcggctggtg accgctggcc
      781 aagatggttt tccgcgacgc gagttccaga cacgctggcg acctttacgg gccattgggt
      841 cccacggagt gtctcgcacc tcggatcgat cgatcctacc atctactcct cttgcagctc
      901 cggcaccggt cgagatggcg agcccgaagg cgcgccgccc gaccgatctc tgttacgcgg
      961 cgtgcgccga tgcggtcggc ttgccgccgg cgtgatagct cgcgcccgtc accaacagct
     1021 tccaagctat gcgggcaatc ttgttggcga gcgctatcgc ggcgagcttc ggcgacgact
     1081 tgcgcgccag cagcgcgagc agccaaggcg atgcccggcc acggcctcgg ctcgcctgct
     1141 ggatcaccgc ggtcgctccc gccaccagca cgctgcgtag cctctcatct ccggcgcggg
     1201 tgatcgtacc gagccgggtc ttgccggcgg tggaatggtc tttcggggtc agcccgatcc
     1261 aggccgcgaa ctgccggccg gacgggaaca gccgtggatc gggtgccttc atgaccagcg
     1321 ccgtggcgcc gatcggcccg atccccggaa tctgtgcaag acgctggctg tcggcattgg
     1381 atcgatgcca ggccatcagc cgggcttcga ttttcttcag ttcggcctgc aattgcgcgt
     1441 actcccggcc atgcatcacg aacagatcgc gcgccagcac cggcaaggtt tggctggcgg
     1501 cgatgtcagc cagtagcggc tcgatcttgt cgagaccctt ggctgccgtg ataccgaact
     1561 ccgccgcata gccgcggatc atattgctga gctgggtgcg ccgggcgacc atctgctcgc
     1621 gcaggcctgc cagcatcagg gccgcctgct gctcttcggt cttcactggc acaaaccgca
     1681 tcgtcggccg gctcatcgcc tcgcacagtc cctcggcgtc ccgtccgtca ttcttgttgc
     1741 gcttgatgta gggcttcacg tgttgcggcg cgatcagctt cacctcgtgg ccgagtttgc
     1801 gcaactccct tgcccagtaa tgcgaagcgc cgcaggcctc catcccgatc acggtcggtg
     1861 ctagtttggc aaaaaacgcc accatctcct tgcggcgcag cttccggcgc agcaccggct
     1921 gctcagcagc atcaacaccg tgcagctgaa aaaaatgctt cgacgtatcc attccaatgc
     1981 ggataatctg gttcacggac ggctcccttg tctgagattt caacaacctc attctggcac
     2041 aatcgatgcc gtagggggcc gtccacacca tcacgccgcg gcagttattg tgtgaatggt
     2101 ctcagcctcc gtgcgtcatc gatggatcac gcggtatggg tccctgcttt cgcagggacg
     2161 acgggtgtga atttcatatt caactgtcaa acagcatgag ggcgcaactc cgcattgccg
     2221 cggcgcgaat gcgcccgggc tgtgcgttac gtttcgccct caagaaatag agggcgcagg
     2281 gaagaccggg tgcg
//
LOCUS       DASQOP010000322         2708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_167227, whole genome shotgun sequence.
ACCESSION   DASQOP010000322 DASQOP010000000
VERSION     DASQOP010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167227"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="VE030_12660"
     CDS             <1..966
                     /locus_tag="VE030_12660"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014896808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HZF24305.1"
                     /translation="LLDRMEVIRLSGYTEDEKINIAMRYLLPKQMKNNGVKEGELSVS
                     ESAIRDIVRYYTREAGVRGLEREISKICRKVVKSLLMKKRDHRVTVSARNLDHYLGVR
                     RFTYGLAEKNNQVGQVTGLAWTEVGGELLTIEAAVMPGKGNIQRTGSLGDVMKESVEA
                     ARSVVRARAARLGIKDDAFEKYDIHVHVPEGATPKDGPSAGIAMTTALVSALTAIPVR
                     ADVAMTGEITLRGEVLAIGGLKEKLLAAHRGGIKTVLIPEENVKDLTEIPDNIKNRLD
                     IHPVKWIDEVLEHALERNPEPLPEKTEAVAIPAVDSTEAVSAVKH"
     gene            1196..1468
                     /locus_tag="VE030_12665"
     CDS             1196..1468
                     /locus_tag="VE030_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HZF24306.1"
                     /translation="MNKFELIEAVAKSADISKAAAGRALEGTVTAIKSSLKKGGMVTL
                     VGFGTFYVGKRAARSGRNPRTGAIIKIKAARVPKFRAGKALKDAVN"
     gene            1555..1630
                     /locus_tag="VE030_12670"
     tRNA            1555..1630
                     /locus_tag="VE030_12670"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1588..1590,aa:Val,seq:tac)
     gene            1725..1801
                     /locus_tag="VE030_12675"
     tRNA            1725..1801
                     /locus_tag="VE030_12675"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1759..1761,aa:Asp,seq:gtc)
     gene            1930..>2708
                     /locus_tag="VE030_12680"
     CDS             1930..>2708
                     /locus_tag="VE030_12680"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZF24307.1"
                     /translation="MYDFIYRHKKWLQIILLVLIVPPFALFGIDYYFRDTGGGGSLAR
                     FGDVRISDLEYSQALRRAQDRMREMSKNNPDPALLNSPQLKESVLNDLIARKVALAHA
                     YKSGMTVSAAELQKIIGAIEAFRDESGRFSQTRYRQLLQGQGMTPVAFENDIRMNLVL
                     EQLRSVTAGSGFLPGSVVERLFKIGEQEREVSQVVFNPVDYRKQVKISEADVEKYYEE
                     HKNEFQIPERVRLEYLVLSPDAVEHAAKVTDEELKNYYQAN"
BASE COUNT          595 a          765 c          853 g          495 t
ORIGIN      
        1 ctgctggacc ggatggaagt gatccgtctg tccggctaca ccgaggacga gaagatcaac
       61 atcgcgatgc gttacctgct gccgaagcag atgaagaaca acggcgtcaa ggagggcgag
      121 ctgtcggtct ccgaaagcgc catccgcgac atcgtccgct actacacgcg cgaggccggc
      181 gtgcgtggcc tggagcgcga aatctcaaag atctgccgca aggtcgtgaa atcgctgctg
      241 atgaagaagc gcgaccaccg ggtaacggtg agcgcgcgca atctcgacca ctacctgggt
      301 gtgcgccgct tcacttacgg cctggcggaa aaaaacaacc aggtgggtca ggttaccggc
      361 ctggcgtgga ccgaggtcgg cggcgagctg ctcaccatcg aggctgccgt catgccgggc
      421 aagggcaaca tccagcgtac cggctcgctt ggcgacgtta tgaaggaatc ggtcgaggca
      481 gcccggtccg tggtgcgcgc gcgcgcggca cggctcggca tcaaggacga cgcgttcgag
      541 aagtacgaca tccacgtgca cgtgcccgaa ggcgcaacgc ccaaggacgg cccctcggcg
      601 ggcatcgcca tgaccactgc gctggtttcc gcattgaccg cgatcccggt gcgtgcggat
      661 gtcgcaatga ccggcgaaat tacgctgcgc ggcgaagtgc ttgcgatcgg gggcctcaag
      721 gaaaagctgc tggcggcgca ccgcggcggc atcaagacgg tcctgatccc tgaagaaaac
      781 gtcaaggatc tgacggagat accggacaac atcaagaatc gtctggacat ccatccggta
      841 aagtggatcg acgaggtgct ggaacacgcg ctcgagcgca accccgagcc cttgccggag
      901 aaaaccgagg cggtggcgat cccggcggtg gactcgaccg aggcggtgag cgcagtcaag
      961 cattgatttt ttgctgcgaa agagcgggaa tgcgataggc attcccgttt tttttgcgac
     1021 gtcacattgc gctgcaaagc cgcgatcccg tggaaactcg cttgacacga ggattttcgc
     1081 cttgttataa agtgcgtccg ggaattcgaa cgccataaca atttttctgt cttcggcccg
     1141 cagtcgaaaa caatttcctg tttctcccgt caacgaataa cgaaagggga cccaagtgaa
     1201 caaattcgaa ctcattgagg ccgttgcgaa gtccgccgac atctccaaag cggccgccgg
     1261 cagggcattg gaaggcaccg taacggcgat caagagctca ctgaagaaag gcggaatggt
     1321 gacgctggtc ggttttggca ctttctatgt gggcaagcgc gcggcacgca gcggccgcaa
     1381 tccccgcacc ggcgcgatca tcaagatcaa ggcggccaga gtacccaagt tccgcgctgg
     1441 aaaagccctc aaggatgcag taaactagcg cgcttcgttg gaccgcacac cggcaattcc
     1501 tccagaattc ggggtaacga accagcagca acgcggagat tccgcggctg ccgcgggtgc
     1561 ttagctcagt tggtagagcg tcgcccttac aaggcgaaag tcgggagttc gagcctctca
     1621 gcacccacca gaaaggcagt gatgagtgat gagagattag ggcggaagca ggtggcttga
     1681 gcggtttgca cgtcaccgat cactcatgac gcattactga atttggagtg gtagttcagt
     1741 tggttagaat accggcctgt cacgccgggg gtcgcgggtt cgagtcccgt ccactccgcc
     1801 agaacacagg gaaggcgaac gcgagttcgc ctttttcatt tgttgtccgg gttgccgctt
     1861 gcgccggcgt ggcggtggca catcaggcat actctccgat cttcgcagtt ttccgggcca
     1921 ggttgacaca tgtacgattt catctacagg cacaagaaat ggttgcagat cattctgctg
     1981 gttctgatcg tgccgccctt cgcactgttc ggcatcgact actattttcg cgataccggc
     2041 ggtggcggct cgctggcgcg gttcggcgac gtccgcatct cggatctgga gtactcgcag
     2101 gcattgcgac gggcgcaaga ccgcatgcgc gagatgagca aaaacaatcc cgatcccgcg
     2161 ctgctcaaca gtccgcagct gaaggaatcg gtattgaacg acctgatcgc acgcaaggtg
     2221 gcactggcgc acgcctacaa gtcggggatg acggtctccg cggcggagtt gcagaagata
     2281 atcggcgcga tcgaggcatt ccgcgacgaa tcggggcgct tttcgcagac gcgctaccgg
     2341 cagttgctgc agggacaggg catgacgccg gtggctttcg agaacgacat ccgcatgaac
     2401 ctcgtgctcg agcagctgcg cagcgttact gccggctccg gcttcctgcc cggctcggtg
     2461 gtcgagcgac tgttcaagat cggggagcag gaacgtgaag tcagccaggt cgttttcaac
     2521 cccgtggact atcgcaagca ggtgaagatc tccgaagcgg acgtggagaa gtattacgaa
     2581 gagcacaaga acgagttcca gatcccggaa cgcgtgcgcc tggagtacct ggtgttgtcc
     2641 ccggatgccg tggaacatgc ggcaaaggtc accgacgagg aattgaagaa ttactaccag
     2701 gcgaacat
//
LOCUS       DASQOP010000323         2588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1579610, whole genome shotgun sequence.
ACCESSION   DASQOP010000323 DASQOP010000000
VERSION     DASQOP010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1579610"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1230
                     /gene="groL"
                     /locus_tag="VE030_12685"
     CDS             <1..1230
                     /gene="groL"
                     /locus_tag="VE030_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211302.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HZF24308.1"
                     /translation="VKGDMIAQVGTISANNDKQIGGIIAEAMKKVGKDGVITVEESKT
                     METTLEVVEGMQFDRGYLSPYFITDPERMECVLEDVRILIHEKKISSMKDLLPLLEQT
                     AKMGKPLLIIAEDVEGEALATLVVNKLRGTLQCAAVKAPGFGDRRKAMLEDIATLTGG
                     KAITEDLGIKLENVKLEDLGRAKKITIDKDNTTIIEGAGKSADIEGRVKQIRSQVENT
                     TSDYDKEKLQERLAKLVGGVAVIKVGAATETELKEKKARVEDAMHATRAAVEEGIVPG
                     GGSALLRCIAVVEKLKLHDDEAVGAGIVRRSLEEPSRQIALNAGHEGAVVIGRIRDSK
                     EENFGFNAETGEYGDMIKMGIIDPAKVTRLALQNAASIAGLMLTTEALIADIKEDTKA
                     AAAGGGAPGAGGMGGMY"
     gene            complement(1563..>2588)
                     /locus_tag="VE030_12690"
     CDS             complement(1563..>2588)
                     /locus_tag="VE030_12690"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006401221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZF24309.1"
                     /translation="IPFSVLDLAPIAAGATPADAFRNSLGLAQHAEEWGYRRFWLAEH
                     HNMPGIASAATAVIIGHIAGGTKTIRVGAGGVMLPNHAPLVIAEQFGTLASLYPGRID
                     LGLGRAPGTDVPTTRALRRDSKSTGDDFPRDVLELQSYFEPAEPGQKIRAVPGAGLHV
                     PIWLLGSSLFGAQLAAMLGLPFAFASHFAPADMMQALHLYRSGFKPSKHLDRPYAMLG
                     ANVIAADTDAEARRLFTSHQQAFTQLRRGMPGQIPPPIDDIETYWSPAEKAMASQSLA
                     CSVVGASRTVERELHEFIEVTKADELMIAALIYDQAARLRSYEITAEVREHLGAEERK
                     VSLPAAV"
BASE COUNT          553 a          821 c          760 g          454 t
ORIGIN      
        1 gtcaagggcg acatgatcgc ccaggttggc accatttccg ccaacaatga caagcagatc
       61 ggcggcatca tcgctgaagc catgaagaag gtcggcaagg atggcgtcat caccgtcgag
      121 gaatccaaga cgatggaaac cacgcttgaa gtagtcgaag gcatgcagtt tgaccgcggc
      181 tacctctccc cctacttcat tactgacccc gagcgcatgg agtgcgtcct cgaagacgtc
      241 cgcatcctca ttcacgaaaa gaaaatcagc tcgatgaagg acctgctccc gctgctcgag
      301 cagaccgcca agatgggcaa gcccctcttg atcatcgctg aggacgtgga aggcgaagcg
      361 ctggccaccc tcgtggtgaa caagctccgc ggcacgctgc agtgcgctgc cgtcaaggcc
      421 cctggctttg gcgaccgccg caaggccatg ctcgaagaca tcgccaccct cacgggcggc
      481 aaggccatca ccgaagacct cggcatcaag ctcgagaatg tgaagcttga ggatctcgga
      541 cgcgccaaga aaatcaccat cgacaaggac aacaccacga tcatcgaagg cgccggcaag
      601 tccgcggaca tcgaaggccg tgtgaagcag atccgctcgc aggtcgaaaa caccaccagc
      661 gactacgaca aggaaaagct ccaggaacgc cttgccaagc tggtcggcgg cgtcgccgtc
      721 atcaaagtcg gcgcagccac cgagaccgaa ctgaaggaaa agaaggcgcg cgtcgaagac
      781 gctatgcacg ccacccgcgc ggctgtcgag gaaggcattg tccccggcgg cggctcggct
      841 ctgctccgtt gcatcgcggt cgtcgaaaag ctcaagctcc acgatgacga agccgttggc
      901 gcaggcatcg tgcggcgctc tctcgaagag ccttcccgcc agatcgctct caacgccggc
      961 cacgaaggcg ccgttgtgat cggcaggatc cgcgactcca aggaagaaaa cttcggcttc
     1021 aacgccgaaa ccggcgagta cggcgacatg atcaagatgg gcatcatcga cccggccaag
     1081 gtcacccgcc tcgcgctgca gaacgcagcg tccatcgcgg gcctgatgct caccactgaa
     1141 gccctcatcg ccgacatcaa ggaagacacc aaggcagcgg cagccggcgg cggagccccc
     1201 ggcgcaggcg gcatgggcgg catgtactaa gttcgccagc tcgcccgtaa cctcaatccc
     1261 aaaagggcgg tcctcacggg ccgccctttt tgctttttcc agcgtaagat gaagacactt
     1321 tgactccacg aagtgtcatc ccgatcctgg cgattatctt aggtctagct attactggac
     1381 tcggcgttac cataatcatt tggatcgtag ctgcccatct tctgggccac ggcctcgggg
     1441 taatagcaat ttgcaaagtc ttcattgccc tcggcgcgac atgcatcttc gagggcgtgc
     1501 gccaactgcg agcaactcgc actagccacc tttgatgtgc ctcacgctgt ctgagtttcc
     1561 gcctaaacgg ccgcaggtaa gctcactttg cgttcttccg cacccaagtg ctccctgacc
     1621 tctgcggtga tttcgtaaga tcgcaagcgt gcggcctggt cgtagataag ggccgcaatc
     1681 atcagttcat cggccttcgt aacctcgatg aattcatgca gttcgcgctc cacggtcctg
     1741 gatgcgccaa ccaccgaaca ggccagcgac tggctggcca tggccttctc cgcgggagac
     1801 caatacgttt cgatgtcgtc gatcggcggc ggaatttgcc ccggcatccc gcgccgaagt
     1861 tgtgtgaagg cttgctggtg cgaggtgaaa aggcggcgcg cctccgcgtc ggtatcagcg
     1921 gcaatgacat tcgcgcccaa catggcgtag ggccggtcga gatgcttcga cggcttgaag
     1981 ccgcttcgat acagatggag cgcctgcatc atgtcggcgg gtgcgaaatg ggacgcaaag
     2041 gcgaacggca atcccagcat ggccgcaagc tgcgcgccga acaagcttga tcccagcaac
     2101 cagatgggta catgcagacc tgcgccggga accgcgcgga ttttctgccc tggttccgcg
     2161 ggttcgaagt acgactgtaa ctccagcaca tctctcggga aatcatcgcc ggtggattta
     2221 ctgtcgcggc gcaatgcccg cgtcgtcggc acatccgttc caggcgcccg gcccaggccg
     2281 agatcgatgc gtccgggata cagcgacgcc agcgtgccga actgctccgc gatcaccagt
     2341 ggcgcatgat ttggcagcat caccccgccg gctcccacgc gaatggtctt tgtgccaccg
     2401 gctatgtgtc caatgatgac cgccgtcgcg gcgctggcaa tgcccggcat gttgtggtgt
     2461 tcggccagcc agaagcgtcg gtacccccat tcttcagcgt gctgcgcgag ccccagggag
     2521 ttacgaaagg cgtccgccgg ggtcgcgccc gcggcaatgg gcgccagatc aagaactgag
     2581 aatgggat
//
LOCUS       DASQOP010000324        11242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2586791, whole genome shotgun sequence.
ACCESSION   DASQOP010000324 DASQOP010000000
VERSION     DASQOP010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11242
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2586791"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /gene="msrP"
                     /locus_tag="VE030_12695"
     CDS             complement(<1..824)
                     /gene="msrP"
                     /locus_tag="VE030_12695"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014615940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HZF24310.1"
                     /translation="MLIRRPYDIKPSEITGQTLYRDRREFIRAAAALGAAGGLAAVGL
                     LTPGAARSAAKFSDLKKGPFATDETLTAFESITNYNNYYEFGTDKEDPAAHSGKLKTR
                     PWTVKVEGEVKKPKTFDIEELLKLAPLEERIYRMRCVEGWSMVIPWVGYPLSELIKRV
                     EPTGNAKFMEFYTLLDAEQMPGQKMPILDWPYVEGLRMDEAMHPLTLLAVGLYGQALP
                     NQNGAPVRTVVPWKYGFKSAKSIVRIRFSEQQPKTAWEMAAPHEYGFYSNVNPKVD"
     gene            complement(973..2415)
                     /gene="mgtE"
                     /locus_tag="VE030_12700"
     CDS             complement(973..2415)
                     /gene="mgtE"
                     /locus_tag="VE030_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HZF24311.1"
                     /translation="MPRVAERERTPGVQQALDQVKALLQKHRIVESMVHKQDMPRHEL
                     VESLVHKQNLAELQKKLDKLHPADVAYILEALPLEERLMVWDLVKAERDGEILLEVSD
                     AVRETLIADMDSQELVAAAEQLDTDQIADLVQDLPQDVVAGVFQSLSVEEREQLRSAM
                     SYPEDSVGALMDFDMVSVRDDVTLEVVLRYLRRMDELPDHTDQLFVVDRDETTKGVLP
                     INRLIVTDPEVMVASVMNADFISFSPDDKAQKAAQAFERYDLVSAPVVDQRNRLAGRV
                     TVDKVVDFIREESEAELLGKAGLREEEDIFASVWKSAKNRWLWLAVNLCTAFFASRVI
                     GVFEGTIEKLVALATLMPIVAGIAGNSGNQTTTLIIRALALGQVTPENARRMILKELA
                     ISLLNGLVWGGVAGLFAWWLYHSVALGVVMMNAMLLNLLVGALVGILVPMAMQRLGRD
                     PAIGSSVLLTFTTDSMGFFIFLGLATVFLL"
     gene            complement(2588..3052)
                     /locus_tag="VE030_12705"
     CDS             complement(2588..3052)
                     /locus_tag="VE030_12705"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZF24312.1"
                     /translation="MKRFLALLFGCAILGIGTAGMARAEECGGPVTADEALAAEDDRY
                     EAQTTNDFAAMEKLFGSDLVYIHSSAVVDSKTTYIDSMRSGAVKYRTMRRSGVTVRTY
                     GCIAILSGQANFDVTVKGQDLSVELRFHTVWAKRDGGLQFVSWQATRVPAPQ"
     gene            complement(3241..4038)
                     /locus_tag="VE030_12710"
     CDS             complement(3241..4038)
                     /locus_tag="VE030_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24313.1"
                     /translation="MIPGIKAIRNLCAVSAFLTAILCASALAQNPPSKDRIYLRDIEG
                     IWINEQYVKALTALRAPHAAAKKTPPVVIAIKREGRAFPIVVTDFNKASVQAVLDIEP
                     DAKPGSYRLVVGAGDRPMTSDEVKYVYLQGTKNAQGRFDRLRFAEIFLMKGRWADYIL
                     LPGEISPYLNRLVLAGHYKDEQGRGWEFSENGEARWPDQKFVYELSLNDPGAGCEYVQ
                     TEDLKEGAQNKRFGYAWKNGKLSIYPARMVDKKVRCDARPLAVLTPQ"
     gene            complement(4065..5486)
                     /locus_tag="VE030_12715"
     CDS             complement(4065..5486)
                     /locus_tag="VE030_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZF24314.1"
                     /translation="MNADEICFLPAGELVRHIHARELSALEVMDAHLAQTERVNPAVN
                     AIVTYLPELGRSGAQAVDAALARGDDPGPLAGLPVAHKDLAVTRGIRTTFGSPIYRDF
                     VPDVDAIIVERLKRAGAVTFGKTNTPEFGAGSQTFNPVFGATRNPYDLSKTCGGSSGG
                     AAVALACGMAPIADGSDLGGSLRNPASFCNVVGMRPSAGRVPNWPGFSAWFPLSVVGP
                     MARTVPDTALMLSAIAGPDPRSPISLQEPGSSFARPLARDFKGTRIAWSPDLGGLPVE
                     PEVAEALNAQRRVFADLGCLVVDATPDLSDADEIFRVMRAWNFELCYGQLLKSRRGLM
                     KDTLIWNIEEGARLTGPQLGAAESKRTQLFHRMREFMDDHDFLVASVAQVLPFDIDVP
                     YVTEINGIRMESYIDWMRSCYYITVTGSPAISVPCGFSRTGLPVGLQIVGRYRDDFGV
                     LQIAHAFEQATGFWKRRPAVAQS"
     gene            complement(5876..6340)
                     /locus_tag="VE030_12720"
     CDS             complement(5876..6340)
                     /locus_tag="VE030_12720"
                     /inference="COORDINATES: protein motif:HMM:NF021495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HZF24315.1"
                     /translation="MIEVLVVYLHFAAMILIAVFLAIEYLICVPGLARERVQLLARID
                     LLYLIAAVLALASGAARLVWFGKGAGFYLHNPVFYIKLALFVAIGLISIPPTMQYLRW
                     MRALKTGAGAVAADFEVLRARRYVLVELVLFAFIPLLAVLMARGIGIQVPVN"
     gene            complement(6351..7838)
                     /locus_tag="VE030_12725"
     CDS             complement(6351..7838)
                     /locus_tag="VE030_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24316.1"
                     /translation="MAADFLYAEVAILRRVAAVCLAVIALSAIEASAQRLHRYTVAID
                     AELTTLNVRACFDGAPPPYLVAESLDASLALMEARVESTRRRIEPSGSISLKTVPDGG
                     CILYAANVSRPIKQHDRTGGKIQHVGADLLTSVGLWLWRPDTLGPDEDIEITFLLPEG
                     ISVSAPWTPAATAPERAVFRIGRGLWDWPASVAFGRFKEREVRVGGARLRLSVLDGSP
                     PADVDQMQAWIADAAQTVADLYGKFPLAQAQILIVPGARGHEPTPRAYVVRGGSPAAH
                     FFINQRRPIREFYDDWTAVHELSHLLLPYVGSEDAWVSEGVATYYQNVLRARGGRMTV
                     LEAWERLHSGFLRGMDSAPGMTLAQATESMYRGGTHMRVYWEGAAMILIADVRLRQLT
                     AGKQSMDTALAALNECCNSTERAWSGRELFDKLDEITGTAVFRELYDQHVASKDFPDL
                     TQAYRALGITASGRSIELAPQGREKQLRDAIMDTGVLVISEIPDM"
     gene            complement(7914..>11242)
                     /gene="metH"
                     /locus_tag="VE030_12730"
     CDS             complement(7914..>11242)
                     /gene="metH"
                     /locus_tag="VE030_12730"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HZF24317.1"
                     /translation="FVVGVLGPTSRTASISPDVNDPGFRNVSFDELVACYGECVKGLL
                     DGGVDLLMVETVFDTLNCKAALFAIDEYFVSNSVRVPVMVSGTITDASGRTLSGQTTE
                     AFWNSISHARPFSVGLNCALGAKDLRPYIEELSKVADVHTSLHPNAGLPNAFGEYDET
                     PEYTASFLSEFAQSGFLNIAGGCCGTTPAHIKAVVEVLKDVAPRKVPVIDKKLRLSGL
                     EPLNISDDSLFVNVGERTNVTGSKAFARLVLAGNYVEALSVARSQVESGAQIIDVNMD
                     EAMLDSKKAMVTFLNLVGSEPDISRVPVMIDSSKWDVIEAGLKCVQGKCVVNSISMKE
                     GEAEFIRQARLVRRYGAAAIVMAFDEKGQADTLARRIEICTRAYHLLVKEVGFPVEDI
                     IFDPNIFAVATGIEEHNNYAIDYVEATRAIRRALPHAKISGGVSNISFSFRGNDPVRE
                     AIHSAFLFHAIKAGMTMGIVNAGQLGVYQEIPKDLLERVEDVLFNRRPDATERLVEFA
                     ESYKAETKVSVEDLSWRKGTVGSRITHALVRGINTFIVEDTEEARKQFDYPIQVIEGP
                     LMEGMNVVGDLFGAGKMFLPQVVKSARVMKEAVAHLIPYIEAEKARSGTSGQSKGKIV
                     IATVKGDVHDIGKNIVSVVLQCNNFDVVNLGVMVPAEKILQTARDENADIIGLSGLIT
                     PSLEEMAHVAKEMERQGFKVPLLIGGATTSRVHTAVKIEPNYSGPTVWVPDASRSVGV
                     CTSLISVENRAEYVRKVKNEAEKTRAQHKGKKGQGPHYPIAEARKHGLKTDWKAYVPP
                     VPVLTGVKAFRDYPLEEIAKVIDWTPFFQTWELAGRYPKILQDPVVGEAARNLFDDAQ
                     RMLARIISEKWLGAAAVIGLFPAGRAGDDVEVYTGESRTKIATTFHYLRQQMVKPLDK
                     PNQCLADLIAPRDSGVRDYIGAFAVTAGIGIDERVAEFEQKHDDYNAIMLKALADRLA
                     EGFAELMHQRVRREFWGYAAAENLGVDDLIAEKYRGIRPAPGYPACPDHVEKGPLFEL
                     LGAPGTAGITLTESYAMLPTAAVSGFYFSHPESQYFAVAKIDRDQVEDYARRKAMSVQ
                     EVERWLAPNLAYEPGAVLAA"
BASE COUNT         2080 a         3594 c         3386 g         2182 t
ORIGIN      
        1 tgatcgacct tggggtttac gttggaatag aatccgtact cgtgcggggc cgccatttcc
       61 caggcggtct tcggttgctg ttcggaaaag cggatgcgca cgatcgactt cgcgctcttg
      121 aagccgtact tccacggcac caccgtgcgg acgggcgcac cattctggtt cggcagcgcc
      181 tggccgtaaa ggcccaccgc cagcagcgta agcggatgca tcgcttcgtc catgcgcagg
      241 ccctcgacgt agggccagtc gaggatcggc atcttctgac ccggcatctg ctcggcatcg
      301 agcagcgtgt agaactccat gaatttggca ttgccggtag gctcgacgcg tttgatcaat
      361 tcggagagcg gatagccgac ccacgggatc accatcgacc agccctccac gcaacgcata
      421 cggtagattc gttcttccag cggggcgagt ttgagcagtt cctcgatgtc gaaggtcttc
      481 ggtttcttca cctcaccttc gaccttcacc gtccacggac gggtcttcag tttgccggaa
      541 tgcgccgccg ggtcttcctt gtcggtgccg aattcgtagt aattgttgta gttggtgatc
      601 gattcgaacg ccgtgagcgt ttcatcggtt gcgaaggggc cctttttgag atcggaaaac
      661 tttgccgccg aacgcgccgc gcccggcgtc agcagtccga ccgcggccaa tccgcctgcc
      721 gcaccgagcg cggcagccgc gcggatgaat tcccgccggt cccgatacag cgtttggccg
      781 gtaatctccg aaggtttgat gtcgtacggg cgtcggatca acattttcgt gctccttgga
      841 tatttccgcc cctttaggca cgggcggttt gtagcttaca acgtcccggg gatttgcgcg
      901 ggcctaggga agctctgcca tggtacggat gaaagcctta cctggatgtc ctgaagtttt
      961 actcgttccc ggctaaagca ggaagacagt tgccagacca aggaaaataa aaaatcccat
     1021 gctgtcggtg gtgaacgtga gcagcacgct ggaaccgatc gccgggtccc ggcccaacct
     1081 ctgcatcgcc atcggaacga gtattccgac cagcgcgccg accagaaggt tcaggagcat
     1141 cgcgttcatc atcaccactc ccagggcaac actgtggtag agccaccacg cgaacaatcc
     1201 cgccacgcct ccccaaacca ggccgttcag caggctgatc gccagttcct tgaggatcat
     1261 ccggcgcgcg ttctcgggcg tcacctgccc cagggcaagc gcgcggatga tgagcgtggt
     1321 ggtctgattg ccggaattgc cggctatgcc ggcgacaatc ggcatgagcg ttgccagcgc
     1381 aaccagtttc tcgatggtgc cctcaaacac gccgatcact cgcgaggcga agaatgccgt
     1441 gcacagattc acagctagcc acagccagcg gttcttggcg cttttccaca ccgacgcgaa
     1501 aatgtcctcc tcttcgcgca aacccgcctt gccgaggagc tcggcctcgg actcctcgcg
     1561 gatgaagtcc accaccttgt ccacggtcac gcgtcctgcc agccggtttc tctggtccac
     1621 caccggcgcc gacaccagat cgtaacgctc gaaggcctgt gcggccttct gcgccttgtc
     1681 gtccggcgag aacgagatga aatccgcgtt catcaccgac gccaccatga cctccggatc
     1741 ggtgacgatc aggcgattga tcggcagcac gcccttggtc gtttcatcgc ggtccaccac
     1801 gaacaactgg tcggtatggt ccggaagttc atccatgcgc cgcaggtaac gcagcaccac
     1861 ctccagcgtg acatcgtcgc gcaccgacac catgtcgaag tccatgagcg cgcccaccga
     1921 gtcttccggg taggacatcg cggaacgcaa ctgctcgcgc tcctcgaccg acagcgactg
     1981 gaatacgccc gcgactacgt cttgcggcaa gtcctggacc aggtcggcaa tctggtcggt
     2041 atcgagttgc tcggccgcag ccaccagctc ctgcgagtcc atgtcggcga tgagggtttc
     2101 ccgcaccgcg tcggagacct cgagcaggat ttcgccatcg cgctcggcct tgaccagatc
     2161 ccataccatc aggcgctctt ccagcggcag cgcttcgagg atgtaggcga cgtcggcagg
     2221 gtggagcttg tcgagcttct tctgcagctc cgcgaggttc tgcttgtgga cgagcgattc
     2281 caccagctcg tggcgaggca tgtcctgctt atggaccatg ctctctacaa tccggtgttt
     2341 ctgcaggagc gcctttacct gatcgagggc ctgctgcacg cctggcgtgc gttccctttc
     2401 cgcgactcgc ggcatttttg tgggctcggg ctggggggac ggcttaaaag gtccggcatt
     2461 gtaggaacaa aggattacgg gagtccaacg ccgcggatgt cttcgttccg cggggcggcg
     2521 tgggcaatcc cccggggcaa aagcgcgcgg cattgcgccg cgcgcattgg agcagggtga
     2581 agcgggttta ttgtggggca ggcacgcggg tggcctgcca ggatacgaac tgcagtccac
     2641 cgtcgcgctt ggcccagacc gtatggaagc gcagttccac ggacaaatcc tggcctttca
     2701 ccgtgacgtc gaagttggcc tggccggaaa ggatggcgat gcagccgtaa gttcgaaccg
     2761 tcacgccgct gcgccgcatg gtccggtact tgaccgcgcc ggaacgcatg gagtcgatgt
     2821 acgtcgtctt gctgtccacc acggcggaag agtggatata gaccaggtcg ctgccgaaca
     2881 gcttttccat ggcggcgaag tcgttggtgg tctgcgcctc gtagcggtca tcctccgccg
     2941 ccagcgcttc atccgccgtt accggacccc cgcattcttc ggcacgtgcc attccggcgg
     3001 tgccgatacc cagaatcgca caaccaaaca gtagagccaa aaaccgtttc atgatttccc
     3061 cccttttcat tcgtttcgtt gtgaatggac gccggcgatg agcgtatgcg caaacaccgg
     3121 ctccggactg ctcgcgctta aacgccgaac gcttgattac ggtgcacacc acccggacgc
     3181 ggcacccgga aaaaaccctg cagcccttct gttgcgcctc cgggtgctcc ttcctgccct
     3241 tcactgcggc gtgagcacag cgagcggcct ggcatcgcag cgtacctttt tgtccaccat
     3301 gcgcgccggg taaatcgaaa gcttgccgtt tttccaggcg tagccaaagc gtttgttttg
     3361 agcgccctct ttgaggtctt cggtctgcac gtactcgcag cccgctcccg gatcattcag
     3421 cgacagttca tagacgaact tctggtccgg ccagcgcgct tcaccgtttt cgctgaactc
     3481 ccagccccgg ccttgctcgt ccttgtaatg gccggcgagg accagccggt ttagataagg
     3541 gctgatctcg ccaggaagca gtatgtagtc cgcccacctg cctttcatca ggaagatttc
     3601 cgcaaagcgc agccggtcga acctgccctg cgcattcttg gtcccctgga gataaacgta
     3661 cttcacctcg tcgctggtca tcggccggtc accagccccc accaccagcc gataggaccc
     3721 gggtttcgcg tccggctcga tgtcgagcac cgcctgcaca gacgccttgt tgaaatcggt
     3781 caccacgatc ggaaaggccc gcccctcgcg tttgatggcg atcaccaccg gcggcgtctt
     3841 cttcgccgcc gcatgagggg cccggagcgc ggtcaacgct tttacatact gctcattgat
     3901 ccagatgccc tcgatgtcgc gcagataaat gcgatccttg gaaggcgggt tctgcgccag
     3961 cgcggaggca cacaagattg ccgtcaggaa cgccgatacg gcgcacaggt ttcgaattgc
     4021 cttgatacca ggaatcatcg ctcgctcttt gggaagaaaa tcactcagga ttgcgcgacc
     4081 gccggcctgc gtttccagaa gccggtcgcc tgttcgaacg cgtgcgcgat ctgcaacacg
     4141 ccaaaatcgt cccggtagcg gccgacgatc tgcagtccga ccggcaggcc ggtgcggctg
     4201 aagccgcacg gaaccgatat cgccggcgag ccggtgaccg taatgtaata gcacgagcgc
     4261 atccagtcga tgtagctttc catcctgatg ccgttgattt cggtcacgta gggcacgtcg
     4321 atgtcgaacg gcagcacctg tgccaccgat gcgaccagaa agtcgtggtc gtccatgaat
     4381 tcgcgcatgc ggtggaaaag ttgggtgcgc ttggactccg cagcgcccaa ttgcgggccg
     4441 gtaagccgcg cgccttcctc gatattccag atcagcgtgt ccttcatcag accgcgcctg
     4501 gatttcagca gttgcccgta gcaaagttcg aaattccagg ctcgcatcac ccggaagatc
     4561 tcatcggcgt cgctcaggtc cggcgtggcg tctacgacca ggcagccgag atcggcaaag
     4621 acccggcgtt gcgcattcaa tgcctcggcc acttccggct ccaccggcag gccaccgagg
     4681 tccgggctcc aggcaatgcg ggtgcccttg aagtcgcgcg ccagcggccg tgcaaacgac
     4741 gaccccggtt cctgaagcga gatcggcgag cgcggatcgg gaccggcaat ggctgaaagc
     4801 atcaacgccg tgtccggcac cgtgcgcgcc atcggtccga ccaccgacag cgggaaccag
     4861 gccgagaagc ccggccaatt cgggacgcgc ccggcggagg ggcgcatgcc aacgacgttg
     4921 cagaagctcg caggattgcg cagcgatccg cccaggtccg agccatccgc gatcggagcc
     4981 atcccgcagg ccagagccac ggctgcaccg ccgctgctgc cgccgcaggt cttggagagg
     5041 tcgtagggat tccgggtggc gccgaacacc gggttgaatg tctgtgaacc ggcgccaaac
     5101 tccggggtgt tggtcttgcc gaacgtgact gcgcccgcgc gcttcagccg ttcgacgatt
     5161 atggcgtcta catcgggcac gaagtcgcga tagatcggcg agccgaaggt agtgcgaatg
     5221 cccctggtca ccgcgagatc cttgtgcgcc accggcaacc ccgcaagcgg cccgggatcg
     5281 tcgccgcggg ccagcgccgc atctaccgcc tgcgcgccgc tgcggcccag ttcgggaagg
     5341 taggtgacga tcgcgttgac tgccggattc accctttccg tctgcgccag atgcgcgtcc
     5401 atgacctcca gcgcggacag ctcacgcgca tgaatgtgcc ggaccagttc gccggcgggc
     5461 agaaaacaga tttcgtcggc attcatggcg gtttcgactt cgggcggttg cgcaaaaagc
     5521 gctcatggta gcggaatccc gcagtttgca ttgatggggc gactcaagcg ggagaattta
     5581 aaaccgaaaa tctcaccacg aaggcacgaa gcacacgaag agatcaaaat ctggttcgga
     5641 tttacttggt gaagttggtt gaatttgtgg taactgcgca aatttccggt agcccatgtc
     5701 gggacccatc tgaagtgccg ttcggatcat gaatttcatt ccggccttta cctttcttcg
     5761 tgttcttcgt gccttcgtgg tcagaagtat ggtttctccc agaataaact gcacttagcg
     5821 agccgcagcg gcgagcgggt gcaacgaccg caaagcttca atatcggaac acgactcaat
     5881 tgaccggaac ctgtatgccg atgccgcgtg ccatcagcac tgccagcaac ggaatgaagg
     5941 cgaacaggac gagctcgact agcacatagc gacgcgcgcg cagcacttcg aagtccgccg
     6001 ccaccgcacc cgcgcccgtc ttgagcgcgc gcatccagcg cagatattgc atcgtgggag
     6061 gaatggaaat caatcctatg gcgacgaaca gtgccagctt gatgtagaag acagggttgt
     6121 ggaggtagaa accggcgccc ttgccgaacc agacgagtcg cgctgctccg ctagcaaggg
     6181 caagcaccgc cgcgatcagg tagaggagat caatgcgtgc aagcagctgc actctttccc
     6241 gcgccagccc gggaacgcaa atcaaatatt cgatcgcgag aaacaccgcg atcaggatca
     6301 tcgccgcgaa atgcaggtaa accaccagga cttcaatcac ggcagatccc tcacatgtcg
     6361 ggaatttcgc tgattaccag caccccggta tccatgattg catcgcgcaa ctgcttctcc
     6421 ctgccctgcg gagccagttc gatgctgcgg ccgctggccg tgatgccgag cgcccgatag
     6481 gcttgcgtca ggtcggggaa atccttcgag gcgacgtgct ggtcgtagag ctcgcgaaac
     6541 accgccgttc cggtgatctc gtcgagcttg tcgaacaact cgcgcccgct ccaggcgcgt
     6601 tccgtcgaat tgcagcattc gttgagcgcg gcgagcgcag tgtccatgga ttgcttaccg
     6661 gcggtaagct ggcgcagccg cacgtcggcg atcagtatca tcgccgcgcc ttcccagtaa
     6721 acgcgcatgt gcgtgccgcc gcgatacatg ctctcggtcg cctgcgcgag cgtcattccg
     6781 ggtgcgctgt ccatgccgcg caggaagccc gagtgcaggc gctcccaggc ttcgagaacg
     6841 gtcatcctgc cgccgcgtgc gcgcagtacg ttctgatagt aggtcgccac accctccgat
     6901 acccacgcat cctcggaacc gacatacggc aggagcaggt gcgacagttc gtgcaccgcg
     6961 gtccagtcgt catagaactc gcggatcggc cgccgttggt tgatgaagaa atgcgcggcc
     7021 ggactgccgc cacgcactac gtaagcgcgc ggcgtcggct cgtggccgcg cgcgccggga
     7081 acgatcagga tctgcgcctg cgccagcgga aatttcccgt aaaggtcggc taccgtctgt
     7141 gccgcgtcgg cgatccatgc ttgcatctga tcgacatccg cgggcggcga tccgtccagc
     7201 accgacagcc gcaagcgcgc gcccccgaca cgcacttcgc gctccttgaa gcggccgaac
     7261 gccaccgacg ccggccagtc ccataacccc cgcccgatgc ggaacacggc acgctcgggc
     7321 gcggtggccg ccggtgtcca cggcgccgag accgaaatgc cctcgggaag caggaaagtg
     7381 atctcgatgt cttcgtcggg gccaagagtg tccggccgcc acagccataa gccaacggaa
     7441 gtcagcagat ccgcgccgac gtgctggatt ttcccgccgg tacgatcgtg ctgtttgatg
     7501 ggccgcgaga cgttggccgc atagaggatg cagcctccgt cggggacggt cttgaggctg
     7561 atcgagccgc tcggctcgat tctacggcgg gtgctctcca cccgcgcttc catcaacgcc
     7621 agggacgcgt ccagcgattc ggccaccaga taaggcggcg gcgcgccatc gaagcatgcg
     7681 cgcacgttca gggtcgtgag ttccgcatcg atggcgacgg tgtacctgtg cagacgctgt
     7741 gccgaagcct cgattgccga aagcgcgatg acggcaaggc acaccgcagc gacgcggcga
     7801 aggattgcaa cttctgcgta tagaaagtcg gccgccattt gcgcaattcc gatcctgcgg
     7861 gatgttacac cgggattgga gcgaagcccc ggcgcgaagt tcgcggacgc cggtcaggcg
     7921 gccagaaccg cgccgggctc gtaggccaga ttgggcgcaa gccacctctc cacctcttgc
     7981 acgctcatgg ctttgcggcg tgcgtagtcc tcgacctgat cgcgatcgat cttcgccacc
     8041 gcgaagtact gcgactcggg gtgcgagaag tagaaaccgc ttaccgccgc ggtcggcagc
     8101 atggcgtagg actcggtgag cgtgatgccg gcggttcccg gcgcgccgag cagttcgaac
     8161 aacgggcctt tctcgacatg atccgggcag gccggatagc ccggtgcagg acggattccg
     8221 cggtacttct cggcgatcag gtcgtcgacg cccaggttct ccgcggcggc gtaaccccag
     8281 aattcccggc gcacgcgttg gtgcatcagc tcggcgaaac cctccgccag ccggtccgca
     8341 agcgccttga gcatgatggc gttgtagtcg tcgtgctttt gctcgaactc cgcaactctt
     8401 tcatcgatgc cgatgccggc ggtcaccgca aaggcgccga tgtagtcgcg cacgccggag
     8461 tccctgggtg cgatgaggtc ggccaggcat tggttgggtt tgtccagcgg cttcaccatc
     8521 tgctgccgca ggtaatggaa ggtagtcgcg atctttgtgc gcgattcgcc ggtatagacc
     8581 tcgacgtcgt cgccggcccg gccggcaggg aacaacccga tcaccgcggc tgcgccgagc
     8641 catttctcgg aaatgatgcg ggccagcatc ctctgcgcgt cgtcgaacag attgcgcgcc
     8701 gcctcgccga ccaccgggtc ctgcaaaatc ttcggatagc ggcccgccag ctcccaggtc
     8761 tggaaaaacg gcgtccagtc gatcactttc gcgatttcct cgagaggata gtcgcggaac
     8821 gctttcacgc cggtaagaac gggaacgggc gggacgtaag ctttccaatc ggtcttcaag
     8881 ccgtgcttgc gggcttcggc gatcggatag tgcggaccct gacccttctt gcccttgtgc
     8941 tgggcgcggg tcttctccgc ttcgttcttt accttgcgaa cgtactcggc gcggttttcc
     9001 acggagatca ggctggtgca cacgccgacc gagcgcgaag cgtccggcac ccataccgtc
     9061 ggccccgaat aattcggctc gattttcacc gcggtgtgca cccgcgacgt ggtggcgccg
     9121 ccgatcagca gcggcacctt gaagccctgg cgctccatct ccttcgccac gtgcgccatt
     9181 tcctccagag acggggtaat caggccggag agcccgatga tgtcggcgtt ctcgtcgcgc
     9241 gcggtttgga gtattttctc cgccggcacc atgacgccga ggttgacgac gtcgaagttg
     9301 ttgcactgga gcaccacgga aacgatgttc ttgccgatgt cgtgaacatc gcccttgacc
     9361 gtggcgatca cgattttgcc cttgctctgt ccactcgtgc cggaccgggc cttttccgcc
     9421 tcgatatacg gaatcaggtg agcgaccgcc tccttcatca cccgcgcgct tttcaccacc
     9481 tgcggcagga acatcttgcc cgcgccgaac aggtcgccga ccacgttcat gccttccatt
     9541 agcgggcctt cgatcacctg gatcggataa tcgaactgtt tgcgcgcctc ctccgtgtcc
     9601 tcgacgataa aggtattgat acctcgcacc agcgcatgcg tgatgcggct gcccaccgtg
     9661 cctttgcgcc acgacaggtc ttcgaccgaa accttcgttt cggccttgta gctctcggcg
     9721 aactcgacca ggcgttcggt ggcatccggc ctgcggttga acaacacatc ctcgacccgc
     9781 tccagcagat ccttgggaat ttcctggtac accccgagct ggccggcgtt aacgatgccc
     9841 atggtcattc ctgccttgat ggcgtggaac aggaacgcgc tgtggatcgc ctcgcgcacc
     9901 ggatcgttgc cgcggaatga gaacgagatg ttcgacaccc cgccgcttat ttttgcgtgc
     9961 ggcagtgccc tgcggatcgc ccgcgtggct tcgacgtagt cgattgcgta gttgttgtgt
    10021 tcctcgatcc cggtggccac ggcgaagata ttcgggtcga atatgatgtc ctcgaccggg
    10081 aacccgactt ccttcaccag caggtggtag gcgcgggtgc atatctcgat gcgccgcgcc
    10141 agcgtatcgg cctggccttt ctcgtcgaag gccatgacga tcgccgctgc accatagcgc
    10201 cgcaccaggc gcgcctggcg gatgaactcg gcttcgcctt ccttcatgct gatcgagttc
    10261 accacgcact tgccctgcac gcatttgaga ccggcctcga ttacatccca tttggacgaa
    10321 tcgatcatga ccggcacgcg cgagatgtcc ggttcggagc cgaccagatt gaggaaagtc
    10381 accatcgcct tcttcgaatc cagcatcgcc tcgtccatgt tgacgtcgat gatttgcgcg
    10441 ccgctctcga cctggctgcg cgccaccgag agcgcctcga cataattgcc ggcgaggacc
    10501 aggcgggcga aggctttcga gccggtgacg ttggtgcgtt cgccgacgtt cacgaacagc
    10561 gagtcgtcgg agatgttgag cggttccagc ccggataggc gcagtttctt gtcgattacg
    10621 ggtaccttgc gcggtgctac gtccttgagc acctcgacaa ccgccttgat atgcgccggc
    10681 gtggtgccgc agcaaccgcc ggcgatgttc aggaacccgc tttgcgcgaa ttcgctgagg
    10741 aaggaggcgg tgtactccgg cgtctcgtcg tactcgccga aggcattcgg cagcccggcg
    10801 ttcgggtgca ggctggtgtg cacatcggcg accttcgaca actcctcgat gtagggccgc
    10861 aggtccttgg caccgagcgc gcaattcagt ccgaccgaga acggccgcgc gtgggaaatc
    10921 gagttccaga acgcttcggt ggtctgtccc gaaagcgtgc ggccgctggc atcggtaatg
    10981 gtgccggaca ccatgaccgg cacgcgcacg ctattactga cgaagtattc gtcgatcgcg
    11041 aacagcgcgg ccttgcagtt gagcgtgtcg aataccgtct cgaccatcag cagatccacg
    11101 ccgccgtcaa gcaagccttt cacacattcg ccgtaacagg ccaccagttc gtcgaaggaa
    11161 acgttgcgaa atcccggatc gttcacgtcc ggcgaaatcg atgcggtgcg gctggtgggc
    11221 ccaagcacgc cgacgacgaa gc
//
LOCUS       DASQOP010000325         7112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1376108, whole genome shotgun sequence.
ACCESSION   DASQOP010000325 DASQOP010000000
VERSION     DASQOP010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7112
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1376108"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..1482
                     /locus_tag="VE030_12735"
     CDS             64..1482
                     /locus_tag="VE030_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HZF24318.1"
                     /translation="MSFDSYYNEMYLADGSVRSHYRPLAEWIAGTPAEHIQQLRQAAH
                     LLFHRVGITFAVYGEEAGAERLIPFDILPHIIPAAEWRDIANGLRQRAKALNAFLHDI
                     YHGQEILKAGKVPSERVLENKQYRPEMAGIDLPGGIYAHIAGIDLVRAGAGEYYVLED
                     NLRTPSGVSYMLENRKMMMRLVPELFARQSIAPVEHYPDMLLENLRSVAPNGVENPTV
                     VLLTPGQFNSAYFEHAFLAQQMGIELVEGADLLIRDDTVYMRTTAGPQRVDVIYRRID
                     DDFIDPQAFRPDSMLGVPGLVNAYRTGRVTLANAIGTGVADDKSIYPYVPEMIRFYLA
                     EEPLLNNVPTYQLRLEQDLVYVLEHLAELVVKEVHGSGGYGMLIGPASTEHERAAYRQ
                     RILDAPDKFIAQPTLSLSTCPIFVEQGIAPRHIDLRPFVLSGKQVMMVPGGLTRVALR
                     EGSLVVNSSQGGGTKDTWIVEN"
     gene            1568..2515
                     /locus_tag="VE030_12740"
     CDS             1568..2515
                     /locus_tag="VE030_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HZF24319.1"
                     /translation="MLSRTASTLYWMARYVERAENTARILDVTYRMSLLPKAAKFSDQ
                     EWFAPLNITGTLYPFSGRYSTVTARDVMHFMALDPANPSSIFSCAKAARESARAVRGN
                     ITSEMWEVINSTWLELQHVTEARLSGERALAFFDWVKDRSHLFRGVTVGTALKDGAFH
                     FNRLGTFLERADNTARILDVKYHVLLPSVEDVGGAVDYYQWAAVLRSVSSFEAYRKVY
                     RDVITPVKVAELLILRQDMPRSLHACMDEAYEIFQMIATRYSGEALRRAGELHAALHF
                     GRMDVIFDHGLHEYLTDFLGRLTELGSEVDRSFFNVAYA"
     gene            2573..3307
                     /locus_tag="VE030_12745"
     CDS             2573..3307
                     /locus_tag="VE030_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-type protease"
                     /protein_id="HZF24320.1"
                     /translation="MTYCVALKLDSGMLFASDSRTNAGVDHIATFSKLKVYEQKDERV
                     LVILSSGNLAVTQGVMNILDRHKNAEPGKDTIWNVGSLFDVATLVGDALRDVQKRDGP
                     YLAQNNIDYNATLLLGGQIKGEEPRLFNVYAAGNFIEASVDTIYFQVGESKYGKPILD
                     RVLTSSTPYQEAAKCALISFDSTIRSNISVGMPIDLLWYPRDSLRAGVQKRIGEGDPY
                     FTMIQKSWGSGLRRVFTELPNPDWLS"
     gene            3472..6834
                     /locus_tag="VE030_12750"
     CDS             3472..6834
                     /locus_tag="VE030_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HZF24321.1"
                     /translation="MTIRVALTHETSYRFDRPVNLSPHEIRLRPAPHCRTPINSYSLK
                     IAPAKHFINWQQDPFGNWLARVVFPDKSAELKIVVDLVADMTVVNPFDFFVESYAEKY
                     PFEYSHQLKAELRPFLETEPLGPLLSDWLAAFRQEALRQPLAIVDLLVAANTRLQRDI
                     AYIVRLEPGIQACEETLGLRRGSCRDSAWLLVQIFRHLGLAARFASGYLIQLTADVKA
                     LDGPSGPETDFTDLHAWAEVYIPGAGWVGLDPTSGLFAGEGHIPLACTALPSSAAPVT
                     GYTDVAEVQFGFHMQVRRIHEDPRVTKPYTGQQWADIEALARKVDGQLAAWDVRLTMG
                     GEPTFVSIDDMDGVEWNVAALGEKKRELAGALLVRLGDKFSCGGLLHYGQGKWYPGEA
                     LPRWALSCFWRADGKLSWKDPALLAGDGKDYGFGTKEAGRFALALTERLGLHADYVIA
                     AYEDVWQTVQQEQKLPVNIDPLDLDLVDSLDRKRLARLLDRELGKEAGYVLPLKPAQR
                     PRAQEAPAWLSSPWPLRREHLYLVAGDSPIGLRLPLDSLPWISPRDIDPRHVRDPFEE
                     REQLHEPAGEVAVKAKKPKPVKLEVGKSAIEIIHTALCVQAREGRLHVFFPPLSAVED
                     WIELVAAVEATAAELKMPVVLEGYAPPFDPRIRRFAVTPDPGVIEVNIHPAATWDELS
                     TLTRVLYEQARLTRLGTEKFMLDGRHTGTGGGNHVTIGGLTPADSPLLRRPDLLRSLI
                     TYWQNHPALSYLFSGMFIGPTSQAPRVDEARDDNLYELEIAFQQMEKKAKPGEEVPQP
                     WLVDRLLRNLLVDVTGNTHRAEFSIDKLYSPDGPAGRLGLLEFRAFEMPPHWQMSLAQ
                     MLLLRTLIARFWKAPHTGKLVAWGTSLHDRFMLPHFVAEDMKDVVSDLRDAGYAFQMD
                     WFAPFFEFRFPRYGTLDCDGMQLELRQALEPWHVLGEEAATSGTSRYVDSSVERMQVK
                     VSNMVAERYIVTCNGRVVPLTPTGTRGEYVGGVRYKAWEPPSALHPTVGVNAPLVFDL
                     VDKWSRRAVGGSTYHVSHPGGRSYAKLPVNANAAEARRVARFMSQGHTPGPVTIKSEA
                     PSADFPLTLDLRRQPDYAVAFEAAPPQAQQQQ"
     gene            6853..>7112
                     /locus_tag="VE030_12755"
     CDS             6853..>7112
                     /locus_tag="VE030_12755"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HZF24322.1"
                     /translation="MDRAWLLGLVLFAAAANATEWVEVGADTEAKYYVDVDSISVDGD
                     NVSVHKRGVYTHVLTDNFDGHPTTFKESIGTVELDCARRINRV"
BASE COUNT         1315 a         2266 c         2257 g         1274 t
ORIGIN      
        1 cgaattggca cagcggttgc tgaatcaatc cggaggtgag gcttcccggt tgaaagcgcg
       61 accatgtcat tcgattccta ctacaacgag atgtacctgg ccgacggaag cgtgcgcagc
      121 cattaccggc cgcttgccga atggattgcc ggcacgccgg cggagcacat ccagcagctg
      181 cgccaggcgg cccacctgct gttccaccgc gttggaatca ctttcgcggt gtacggggag
      241 gaggccgggg ccgagcggct gatcccgttc gacatactgc cgcacatcat tcccgcagcg
      301 gagtggcgcg acattgccaa cggcctgcgc cagcgcgcca aggcgctgaa cgcctttctg
      361 cacgacatct accacggcca ggaaattctc aaggccggca aggtgccgag cgagcgggtg
      421 ctggaaaaca agcagtaccg gccggaaatg gccggcatcg acctgcccgg cggcatctac
      481 gcgcatatag cggggatcga tctggtgcgc gcgggggccg gcgagtacta cgtgctcgag
      541 gacaacctgc gcaccccgtc cggggtgtcg tacatgctgg agaaccgcaa gatgatgatg
      601 cggctggtgc ccgagctgtt cgcacgccag tcgatcgcgc cggtcgagca ctaccccgac
      661 atgctgctgg aaaacctgcg cagcgtggcg cccaacggcg tcgagaatcc caccgtggtg
      721 ctgctcacgc cgggccagtt caacagcgcc tacttcgagc acgccttcct ggcgcagcag
      781 atgggcatcg aactggtgga gggggcggac ctgctcatcc gcgacgatac cgtttacatg
      841 cgcaccaccg ccgggccgca acgggtggac gtgatctacc gccgcatcga cgacgacttc
      901 atcgacccgc aggcgttccg tcccgattcc atgctcggcg tgcccggcct ggtgaacgcg
      961 taccgcaccg gtagggtgac gcttgccaac gcaatcggca ccggcgtggc cgacgacaag
     1021 tccatctatc cctatgtacc ggagatgatc cgcttttatc tcgccgagga accgctgctc
     1081 aacaacgtgc cgacctacca gctgcgcctt gagcaggatc tcgtttacgt gctcgaacac
     1141 ctcgccgagc tggtggtgaa ggaagtgcac ggctcgggcg gctacggcat gctgatcggg
     1201 ccggcctcga cggaacacga gcgcgcggcg tatcgccagc gcattctcga cgcgcccgac
     1261 aagttcatcg cgcagccgac gttgtcgctt tcgacctgcc cgattttcgt cgagcaggga
     1321 attgccccgc gccacatcga tctgcgcccc tttgtgcttt ccggaaagca ggtgatgatg
     1381 gttcccggcg ggctaacccg cgtggccttg cgcgagggtt cgctggtggt gaattcctcg
     1441 cagggcggcg gtaccaaaga cacatggatc gtcgaaaact aggggcgagg gacgaggaac
     1501 gaggggtgag aaaagaccgg attcctactc atccctcatc cctcatccct catccctcgc
     1561 gaagctcatg ctgagccgca ccgcctcgac tctttactgg atggcgcgct acgtcgagcg
     1621 tgcggagaac actgcgcgca tcctcgacgt gacctatcgc atgtcgctgc tgccgaaggc
     1681 ggccaagttt tccgatcagg agtggttcgc gccgctgaac atcaccggca cgctttaccc
     1741 cttctccgga cgctacagca cggtcaccgc cagggacgtc atgcacttca tggcgctcga
     1801 cccggccaat ccgtcgtcga ttttttcctg cgccaaggcc gcgcgcgaga gcgcccgcgc
     1861 ggtgcgcggc aacattacct cggaaatgtg ggaggtgatc aattccactt ggctggagct
     1921 gcagcacgtc accgaggcgc ggctttccgg cgaacgggcg ctggcgttct tcgactgggt
     1981 gaaggatcgt tcgcacctgt ttcgcggcgt tacggtgggc accgcgctca aggacggagc
     2041 cttccacttc aatcgcctgg gtacattcct ggagcgggcc gacaacaccg cgcgcatcct
     2101 ggacgtgaag taccacgtgc tgctgccctc ggtggaggac gtcggcggcg ctgtggatta
     2161 ttaccagtgg gcggcggtgc tgcgttcggt ttcctccttc gaggcctacc gcaaggtcta
     2221 tcgcgatgtc atcacgccgg tcaaggtggc ggaactgctg atcctgcgcc aggacatgcc
     2281 gcgatcgctg catgcctgca tggacgaagc ctacgaaatc ttccagatga tcgccacacg
     2341 ctactccggc gaggcattgc ggcgcgccgg cgagctgcat gcagcactgc acttcgggcg
     2401 catggacgtc atcttcgatc atggcctgca cgaatacctt accgacttcc tcggcaggct
     2461 caccgaactg ggcagcgaag tggaccgcag tttcttcaat gtggcctacg cgtgagcggc
     2521 gccgggtggc gctgaggtat catgaaaatc cctgctgcct gacggacatt tcatgaccta
     2581 ttgcgtcgca ctgaagctcg actccgggat gctgtttgcg tccgattcgc gcaccaacgc
     2641 cggggtagac cacatcgcca ccttcagcaa gctgaaggtc tacgagcaga aggacgagcg
     2701 cgtgctggtc atcctttcct cgggaaatct cgccgttacc cagggggtga tgaacatcct
     2761 cgaccggcac aagaatgccg aacccgggaa agacacgatc tggaacgtcg gcagtctctt
     2821 cgatgtcgcc acgctggtgg gcgatgcgct gcgcgacgtg cagaagcgcg acggaccgta
     2881 tcttgcgcag aacaacatcg attacaacgc cacgcttctg ctgggcggcc agatcaaggg
     2941 cgaagagccg cggcttttca acgtctacgc ggcgggaaat ttcatcgagg ccagcgtcga
     3001 caccatttac ttccaggtcg gcgaaagcaa gtacggcaaa cccatcctcg accgcgtgct
     3061 gaccagttcc acgccatacc aggaagcggc gaagtgcgcc ctgatttcgt tcgattcgac
     3121 catccgcagc aatatttccg tcggcatgcc gatcgatctg ctgtggtatc cgcgcgattc
     3181 gttgcgcgcc ggcgtacaga agcgcatcgg cgagggcgat ccctacttca ccatgatcca
     3241 gaagagctgg ggcagcgggc tgcggcgcgt gttcaccgag ttgccgaatc ccgactggct
     3301 gtcctagacc gatttcacca cagagacacg gagacacaga ggaaaaccaa attgggtgac
     3361 aagaacgatg gcagcgccgg gacgacatca cgtagtgggt acgcctacac cggattttcg
     3421 agtatttcga ttctctctgt gcctccgtgt ctctgtggtt agttcaaatc catgaccatc
     3481 cgcgtagcgc tgacccacga gaccagctat cgcttcgacc gcccggtcaa cctgtccccg
     3541 catgagatcc gcctgcgtcc ggcaccgcat tgccgcacgc caatcaacag ttattcgctg
     3601 aagatcgcgc ctgccaagca cttcatcaac tggcagcagg atccgttcgg caactggctt
     3661 gcgcgcgtgg tgtttcccga caagagcgcc gagctgaaga tcgtggtgga cctggtggcc
     3721 gacatgacgg tcgtcaatcc tttcgacttc tttgtcgaga gctacgccga gaagtacccc
     3781 ttcgagtatt cccatcagct gaaagccgaa ctgcggcctt ttctcgagac cgagccgctg
     3841 gggccgctgc tatcggactg gctggcggcg ttcaggcaag aggcgctgcg ccagccgctg
     3901 gccatcgtgg atctgctggt ggccgcgaac acgcgcctgc aacgcgacat cgcctatatc
     3961 gtgcggctcg aacccggcat ccaggcctgc gaggagacgc tcgggctgcg ccggggttcg
     4021 tgccgcgact ccgcctggct gctggtgcag attttcaggc atctcggcct tgccgcgcgc
     4081 tttgcgtccg gctatctgat ccagctcacc gctgatgtaa aggcgctgga cgggccgagc
     4141 gggccggaga cggacttcac cgatctgcat gcgtgggccg aggtctacat tcccggggcc
     4201 ggctgggtgg ggctggatcc gacctccggg ctgtttgccg gcgagggcca cattccgctc
     4261 gcctgcaccg cgttgccgtc gagcgctgcg ccggtgactg ggtacaccga cgtggccgaa
     4321 gtgcagttcg gcttccacat gcaagtccgg cgcatccacg aagatccgcg tgtcaccaag
     4381 ccgtataccg gccagcaatg ggcggacatc gaggcgctgg cgcgcaaagt ggacggccag
     4441 ctggcggcct gggatgtacg gctcaccatg ggaggcgagc cgactttcgt atcgatcgac
     4501 gacatggacg gcgtggaatg gaatgtggcg gcgctgggcg agaaaaaacg cgagcttgcg
     4561 ggcgcgctgc tcgtgcgcct cggggacaaa ttctcctgcg gcgggctgct gcactacggc
     4621 cagggcaagt ggtatccggg ggaagcgttg ccgcgctggg cgttgagctg cttctggcgc
     4681 gccgacggca agctctcgtg gaaggatccg gcgcttctcg ccggggatgg caaggactac
     4741 ggattcggaa ccaaggaagc gggccgcttc gcgctggcgc tcactgaaag actcggattg
     4801 catgccgact atgtgattgc cgcttacgaa gacgtctggc agaccgtgca gcaggaacag
     4861 aaactgccgg tcaacatcga tccgctggac ctggatctgg tcgattcgct cgacaggaag
     4921 cgcctggcgc ggctgctcga tcgcgaactc ggcaaggaag ccggttacgt cctgccgttg
     4981 aagccggcgc aacggccgcg agcgcaagag gcgccagcgt ggctgtcgag cccctggccg
     5041 ctcaggcgcg aacacctgta tctggttgcc ggagattccc cgatcggact gcggctgccg
     5101 ctggattcgt tgccctggat ctcgcccaga gacatcgacc cgcgccacgt tcgcgatccg
     5161 ttcgaggaac gcgagcaatt gcacgagccg gcaggcgaag tcgcggtgaa ggcgaagaag
     5221 cccaagcccg tgaaactcga ggtcggcaaa tcggcgatcg agatcatcca taccgcgctg
     5281 tgcgtgcagg cacgggaagg gcggctgcat gtattcttcc cgccgctgtc cgcggtcgaa
     5341 gactggatcg agttggtcgc ggcggttgaa gccactgcgg ccgaattgaa aatgccggtc
     5401 gttctggagg gttatgcgcc gcctttcgat ccgcgcatcc gccgctttgc ggtcacgccc
     5461 gaccccggcg tgatcgaagt aaacatccat ccggccgcga cctgggacga actcagcacg
     5521 ctcacccggg tgctctacga acaggcgcgg ctcacccggc tcggcaccga aaaattcatg
     5581 ctggacggcc ggcacaccgg taccggtggc ggcaaccacg taaccatcgg cgggcttact
     5641 ccggcggaca gcccgctgtt gcggcgcccg gacctgctgc gcagcctcat cacctactgg
     5701 cagaaccatc cggcgctgtc gtatctgttc tcgggcatgt tcatcggccc gaccagccag
     5761 gcaccgcggg tggacgaggc gcgcgacgac aatctctacg aactcgagat cgctttccag
     5821 cagatggaga agaaagccaa gcccggcgaa gaagtgcctc agccctggct ggtggaccgg
     5881 ctgttgcgaa acctgctggt ggacgtcacc ggcaataccc atcgcgccga attctcgatc
     5941 gacaagctct actctcccga tggaccggcg gggcggctcg ggcttctgga gttccgcgct
     6001 ttcgagatgc cgccgcactg gcagatgagc ctggcgcaga tgctgctgct gcgcacgctg
     6061 atcgcgcgct tctggaaagc gcctcatacc gggaaactgg ttgcgtgggg caccagcctg
     6121 cacgatcgct tcatgctgcc gcatttcgtc gccgaagaca tgaaggatgt cgtgtccgat
     6181 ttgcgcgatg cgggctatgc ctttcaaatg gactggttcg cgccgttctt cgagtttcgt
     6241 ttcccacgct acggcaccct ggactgcgac ggcatgcagc tggaactgcg ccaggcgctg
     6301 gaaccctggc acgtgctcgg cgaggaggcc gcgaccagcg gcacttcgcg ctacgtcgat
     6361 tcctcggtcg agcgcatgca ggtgaaagtc agcaacatgg tcgccgagcg ttacatcgtc
     6421 acctgcaatg gccgggtcgt gcccctgacc ccgaccggca cccgcggcga atatgtcggc
     6481 ggcgtgcgtt acaaagcctg ggagccgccg tctgcgctgc acccgaccgt cggcgtcaat
     6541 gcgccgctcg tgttcgacct ggtcgacaag tggagccggc gtgcggtcgg cggcagcacc
     6601 taccacgtgt cgcacccggg cgggcgcagc tacgcaaaac tgccggtgaa tgcgaacgcg
     6661 gccgaggcac ggcgcgtcgc ccgcttcatg tcgcaggggc acactccggg accggtgacg
     6721 atcaagtccg aagcgccgtc ggcggacttc ccgctcaccc tcgacctgcg ccgccagccg
     6781 gattacgctg tggccttcga agcggcgccg ccacaggcgc agcaacaaca atagtccgga
     6841 gcgcaaaggc aaatggatcg ggcgtggctg ttagggctgg tgctgttcgc agcggcggcg
     6901 aacgcgaccg agtgggtgga ggtcggcgcg gataccgaag cgaaatacta cgtcgacgtg
     6961 gactcgataa gcgtcgacgg cgacaacgtc agcgtgcata agcgcggcgt ctacacccac
     7021 gtgctcaccg acaacttcga cggccatccg accacgttca aggaatccat cggcaccgtc
     7081 gagctggatt gcgcccggcg catcaatcgc gt
//
LOCUS       DASQOP010000326         2020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1176918, whole genome shotgun sequence.
ACCESSION   DASQOP010000326 DASQOP010000000
VERSION     DASQOP010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176918"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VE030_12760"
     CDS             complement(<1..275)
                     /locus_tag="VE030_12760"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HZF24323.1"
                     /translation="MNVIQPQINGPLKVSGDIEIVAADGTVLKRVTEAWLCRCGQSAN
                     KPFCDSSHKAAGFRDPVAVSPEYQPKALDPGAPGAQLRITLKANGPLR"
     gene            complement(371..1153)
                     /locus_tag="VE030_12765"
     CDS             complement(371..1153)
                     /locus_tag="VE030_12765"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HZF24324.1"
                     /translation="MLRGKAVVITGSTSGIGLGIAWAFAAQGCDVMLNGFGDATEIEG
                     LRAGLAAEHKVAVLYSSADMAQPAEIVAMIDQAKRRFGAVDVLVNNAGIQHVAPVEEF
                     PIEKWDAILAINLSSAFHAIRAALPGMKSKGWGRIINVASAHGLVASPFKSAYIAAKH
                     GLVGLTKTVALETAGKGITCNAICPGYVLTPLVEKQIDDQAKVHGIAREDVIRDVILE
                     RQPSKEFVKVEEVAALAVFLGGDSAASITGAALPVDGGWVAQ"
     gene            complement(1224..>2020)
                     /locus_tag="VE030_12770"
     CDS             complement(1224..>2020)
                     /locus_tag="VE030_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZF24325.1"
                     /translation="LQAGGHRLEYHFLHPEGAPDAGRAPLVFLHEGLGSVALWKDFPA
                     QVVSATGAPALVYSRYGYGKSDRLAGARGVDYMHREALEVLPDLLDALSIRNPVLIGH
                     SDGASIALIHAGSGSRPVRGLVLMAPHVFVEDITVKSIAEAKIAFENTGLPAKLARYH
                     DDAESTFRGWNDIWLHPDFRFWNIEEYLPGVNSPVLLIQGEDDQYGTAAQVKAIASQV
                     NGPVETLMLRNCAHSPQVDQMQATVERIAHFVARLGDKPSSTERRP"
BASE COUNT          344 a          661 c          624 g          391 t
ORIGIN      
        1 cgcagcggcc cgttggcctt gagggtgatg cgcagctgtg cgcccggcgc gccgggatcg
       61 agcgccttgg gttgatattc gggggagacg gcgaccgggt cgcgaaagcc cgccgccttg
      121 tggctgctgt cgcaaaaggg cttgttcgcc gattggccgc agcggcacag ccacgcctcg
      181 gtcacgcgct tgagcaccgt gccgtcggcc gcgacgatct caatatcgcc gctgactttc
      241 agcggtccat tgatctgagg ttgaatgacg ttcatgtccg tggctacgtt ggtatggatt
      301 atctgcagtt acccttcact cattcactcc ttcacccctt cactcattca cccttcacgc
      361 cttcaccggc ttattgtgcc acccatccgc cgtcgaccgg cagcgccgcg ccggtaatcg
      421 aggcggcgga atcgccgccg agaaacaccg caagcgcggc cacttcctcg actttgacga
      481 actccttgct cggctggcgc tcgaggatga cgtcccggat gacgtcctcg cgcgcaatgc
      541 cgtgcacctt cgcctggtca tcgatctgct tctccaccag cggcgtcagc acgtaccccg
      601 ggcagatcgc gttgcaggtg atgcccttgc ccgcggtctc gagcgccacg gtcttggtga
      661 gtccgaccag accgtgcttg gcggcgatat acgccgattt gaacggggaa gcaaccagtc
      721 cgtgagcgga ggccacgttg atgatgcggc cccagccctt gcttttcatt ccgggaagcg
      781 cggcgcggat ggcatgaaac gccgatgaca ggttgatcgc gaggatcgcg tcccactttt
      841 cgatggggaa ttcctccacc ggcgccacgt gctggatgcc ggcgttgttg accagcacat
      901 ccacggcgcc gaagcgccgc ttcgcctgat cgatcattgc cacgatctcg gcgggttggg
      961 ccatgtcggc cgatgagtac agcacggcta ccttgtgttc ggcggccaat ccggcgcgca
     1021 atccttcgat ttctgtagcg tcgccaaagc cgttgagcat gacatcgcat ccctgggcgg
     1081 cgaatgccca ggcgatgccc agaccgatgc cgctggtgga cccggtaatg acaaccgcct
     1141 tgcctctcaa catgctatac gggcgacccc gcgttcgacg ttgccgcgca gtctagcaga
     1201 agcaatgcgg caatcggtgc tgttcacggc cgtctttctg tggacgatgg cttgtccccc
     1261 agccgggcga cgaagtgcgc gattctctca acggtcgcct gcatctggtc cacttgaggc
     1321 gagtgcgcgc agttccggag catcaatgtt tcgactgggc cgttcacctg actcgcgatc
     1381 gccttcacct gggcggcagt cccgtattgg tcgtcctcgc cctggatcaa cagcaccggg
     1441 ctgttgacac ccggcaggta ctcctcgatg ttccagaagc gaaagtccgg gtgcagccag
     1501 atatcgttcc agccgcggaa cgtcgattcc gcgtcgtcgt ggtagcgcgc gagctttgcc
     1561 ggcaggccgg tattctcgaa cgcgatcttc gcctcggcga tgctctttac cgtgatgtcc
     1621 tcgacgaaaa cgtgcggcgc catcagcacc aggccgcgca ccgggcggct tccggagccg
     1681 gcatggataa gggcgatcga agcgccgtcg ctgtgaccga tcaggaccgg gttgcggatg
     1741 gaaagcgcgt cgagcaaatc cggaagcact tcgagcgcct cgcggtgcat gtaatccacg
     1801 ccacgcgccc cggcaaggcg gtcggacttg ccgtacccgt atcgcgaata gaccagtgcc
     1861 ggagcgcctg tggcgctgac gacctgcgcg ggaaaatcct tccacagcgc gaccgatccc
     1921 agtccttcgt gaagaaacac cagtggcgcg cgccccgcat cgggcgcccc ttcaggatgg
     1981 agaaagtgat actcgagacg gtggccgcca gcctgcagga
//
LOCUS       DASQOP010000327         2951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1177193, whole genome shotgun sequence.
ACCESSION   DASQOP010000327 DASQOP010000000
VERSION     DASQOP010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177193"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VE030_12775"
     CDS             complement(<1..319)
                     /locus_tag="VE030_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HZF24326.1"
                     /translation="MLIEVKVPQLSESVAEATLLSWHKKQGEPVKRDENLIDIETDKV
                     VLELPAPDSGVLSKVMKGDGSTVVSNEVIATIDTDGKAVAGPAASATTAAAAPVKAQE
                     KPAA"
     gene            complement(339..>2951)
                     /locus_tag="VE030_12780"
     CDS             complement(339..>2951)
                     /locus_tag="VE030_12780"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HZF24327.1"
                     /translation="QLVAEYRFRGVLLAQLDPLKRREPPHIAELEPAYYDLTEADMDT
                     VFNTGSLIGPEQATLREIIKALQETYCGTLGVEYMYMSSRVEKVWIQERLEPIRSKPS
                     YSPDYKRHILERLTAAEGLERHLHTRYVGQKRFSLEGSEMLIPMLDNLLQRAGEAGVQ
                     ELVIGMAHRGRLNVLVNTLGKMPKDLFTEFEGKHDDALLAGDVKYHQGFSSDIMTPGG
                     PMHLTLAFNPSHLEIVNPVVEGSVRARQHRRKDRVGREVLPVLIHGDAAFAGQGVVME
                     TLNLSQTRGYGTAGTVHIVVNNQIGFTTSDPRDSRSTLYCTDVAKMVEAPIFHVNGDD
                     PEAAIMAIEIALDYRTKFKKDVVVDLVCFRKLGHNEQDEPMVTQPLMYKIINAHPGTR
                     KLYVDKLAAQGVIGADDADQMMTSYRQALEAGYHTNKTILSNYKPPFAVNWSPYKNTK
                     WNENDDTTLPLADLQMLAEKLTTIPPNFKLHPRVEKIIADRRLMGQGKMPVDWGMAEN
                     LAYASLLKDGFAVRISGQDSSRGTFFHRHAVLHDQNREKWDSGTYTPLQHIAPNQGDF
                     LVVDSVLSEEAVLGFEYGYTTSEPNELVVWEAQFGDFANGAQVVIDQFIAAGEVKWGR
                     ICDLVMLLPHGYEGQGPEHSSARLERYLQLCADYNIQVCVPATPVQMFFLLRRQMIRP
                     YRKPLIIFTPKSLLRHKESVSPLEEFAESKFKPVNGEWDTDIKPENVKRVIFCSGKVF
                     YDLRAGRQERGIKDMAIVRIAQLYPFPHDDFQEQINLYSNAREVLWCQEEPGNQGAWH
                     RIQHYLLRHMRSDQLLGYALRPSSASPAVGYLSLHNQQQKEVVDAAFREIIDNYVLPV
                     QRAGRAVRDPRNDI"
BASE COUNT          500 a          901 c          912 g          638 t
ORIGIN      
        1 cggcagccgg tttctcctgc gccttgaccg gcgccgcagc cgcagtcgtt gcggatgcgg
       61 ccggcccggc aaccgccttg ccatcggtgt cgatcgtggc aatgacttcg ttgctcacga
      121 cggtgctgcc atcgcccttc atgaccttgg agagcacgcc ggaatccggc gccggcagct
      181 ccagcaccac cttgtcggtt tcgatatcga tcaggttctc gtcgcgcttg acaggctcac
      241 cctgcttctt gtgccaggac agcaatgtcg cctccgcaac cgattccgag agttgcggta
      301 ctttgacttc aatcagcatg ctttttccta tcgtgaactt agatgtcgtt gcgcggatcg
      361 cgcacggctc tgccggccct ctgtaccgga agaacgtagt tgtcgatgat ttccctgaag
      421 gcggcgtcga ccacttcctt ctgctgctgg ttgtgcaatg acaggtagcc gaccgccgga
      481 gacgccgacg acggccgcag cgcatatccc agaagctggt cggagcgcat atggcgtagc
      541 aggtagtgct ggatacggtg ccacgcgccc tggttgccgg gctcttcctg gcaccacagg
      601 acctcccggg cattgctgta gaggttgatc tgctcctgga aatcatcatg cggaaaggga
      661 tagagctggg cgatgcgcac gatggccatg tccttgatgc cgcgctcctg gcgtccggcg
      721 cgcaggtcgt agaacacctt gccgctgcag aaaatcacgc gcttgacgtt ctccggcttg
      781 atgtcggtat cccattcgcc gttgaccggt ttgaacttgc tttcggcgaa ttcttccagc
      841 ggtgaaaccg actccttgtg gcgcaacagg ctcttcggag tgaagatgat caacggtttg
      901 cggtacgggc gaatcatctg gcggcgcagc aggaagaaca tctgcaccgg cgttgccggc
      961 acgcatacct ggatattgta gtcggcgcac aactgcagat agcgttccag tcgcgccgaa
     1021 gaatgttcgg gtccctgtcc ttcgtagccg tgcggcagca gcatcaccaa atcgcagatc
     1081 cggccccact tgacctcgcc ggcggcaatg aattgatcga tcaccacctg ggccccgttg
     1141 gcgaagtcgc cgaactgcgc ttcccagacc accagctcgt tgggctccga tgtcgtgtaa
     1201 ccgtattcga aaccgagcac ggcttcctcc gagagcaccg agtcgacgac caggaaatcg
     1261 ccctggttgg gggcaatgtg ctgcagcggc gtgtaggtac cggagtccca tttctcgcgg
     1321 ttctgatcgt gcagcactgc gtggcgatgg aaaaaggtgc cacggcttga gtcctggccc
     1381 gaaatgcgca ccgcaaagcc gtccttgagc aaggaggcgt aggccaggtt ttccgccatg
     1441 ccccagtcca ccggcatctt gccctgcccc atcaggcggc ggtcggcgat gattttctcg
     1501 acccgcggat ggagcttgaa gttgggtggg atggtcgtca gtttctccgc cagcatctga
     1561 aggtcggcga gcggcagcgt agtgtcgtcg ttctcgttcc acttggtgtt tttgtacggg
     1621 ctccagttca ctgcaaacgg cggtttgtag ttcgacagga tggtcttgtt ggtgtgatag
     1681 ccggcctcga gtgcctggcg gtaagacgtc atcatctggt ccgcatcgtc tgcgccgatc
     1741 acgccctgcg ccgcgagttt gtcgacgtac agcttgcgcg ttcccgggtg cgcgttgatg
     1801 atcttgtaca tcagcggctg cgtgaccatc ggctcgtcct gctcgttgtg gccgagcttg
     1861 cggaagcaca ccagatcgac aaccacgtcc ttcttgaact tggtccggta gtccagggca
     1921 atttcgatcg ccatgatcgc tgcctcggga tcgtcgccgt tgacgtggaa aatcggcgct
     1981 tcgaccatct tcgcgacatc ggtgcagtag agcgtcgagc ggctgtcgcg cgggtcggat
     2041 gtggtgaatc cgatctggtt gttgacgacg atgtgtacgg tgcccgcggt tccatagccg
     2101 cgtgtttgcg aaaggttcaa ggtctccatc accacgccct ggccggcgaa ggcagcgtcg
     2161 ccatggatca gcaccggcag cacttccctg cccacccggt ccttgcggcg gtgctggcgc
     2221 gcccgaaccg agccttcgac caccggattc acgatctcga gatgcgacgg attgaacgcc
     2281 agagtcaagt gcatcggccc gcccggcgtc atgatgtcgg acgagaatcc ctggtgatac
     2341 ttgacgtcgc cggcaagcag cgcgtcgtcg tgcttgccct cgaattcggt gaacaggtct
     2401 ttgggcatct tgcccagcgt gttgaccagc acgttgagcc ggccacggtg ggccatgccg
     2461 atcaccagtt cctgcacccc ggcctcgccg gcgcgctgca ggagattgtc cagcatcgga
     2521 atcagcattt cgctgccttc gagcgagaag cgcttctgcc cgacatagcg ggtatgcagg
     2581 tgcctctcca gtccctcggc cgctgtgagg cgctccagaa tatgccgctt gtaatccggg
     2641 gagtagctgg gcttggaccg aatgggctcg agccgttcct ggatccacac cttctccacc
     2701 cgactgctca tgtacatgta ctcgacgccg agggtgccgc agtaggtctc ttgcagcgcc
     2761 ttgatgatct cgcgcagcgt cgcctgctcg gggccgatta atgagccggt attgaacacc
     2821 gtgtccatgt cggcttcggt caggtcgtaa tacgcgggtt ccagctcggc gatatgcggg
     2881 ggctcgcgcc gcttgagcgg gtcgagttgg gcgaggagca cgccgcggaa acggtactcc
     2941 gcgaccagct g
//
LOCUS       DASQOP010000328         9899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_768010, whole genome shotgun sequence.
ACCESSION   DASQOP010000328 DASQOP010000000
VERSION     DASQOP010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9899
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768010"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            656..1381
                     /locus_tag="VE030_12785"
     CDS             656..1381
                     /locus_tag="VE030_12785"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006404027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HZF24328.1"
                     /translation="MRYELYYWPAIQGRGEFVRLALEEAGANYLDVARLAGHGKGVPA
                     LMRFLESTGVERPPFAPPFLKAGDLVIGQTANILSYLGPRLGLCPKAEASRLWAHQLQ
                     LTVTDFLVEIHDTHHPIASSLYYRQQKTEARRMAKHFTRDRLPTFLDYFETVLARNSS
                     GRGFMLGRGLSYVDLSMFQLVAGLRYAFPKTMARVEPDVPGLAALHDRVSARPNIAAY
                     LASGRRIPFNQNGIFRHYPELDV"
     gene            complement(1395..1859)
                     /locus_tag="VE030_12790"
     CDS             complement(1395..1859)
                     /locus_tag="VE030_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24329.1"
                     /translation="MRTDALLWAPLLFLAASAAYADHGALNPVVRQETIDQTICVPGY
                     AKGVRPATRYTNGVKQMLFQRAHMNPALAQTYELDHIIPLALGGHPRKVENLALQPWE
                     GENGAKRKDRLEVKLQCLVCSNQVTLREAQQEILEDWQAAYHKYAPLKCRRR"
     gene            complement(2530..3840)
                     /locus_tag="VE030_12795"
     CDS             complement(2530..3840)
                     /locus_tag="VE030_12795"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZF24330.1"
                     /translation="MDTTPQIITALLALGMALAFIVADRQSPTSRMLAFFLTAVAIAI
                     VVSALIELPLIRGQAYPWWGGILAWPEVLAFVFAYEWVLRVRRTIPARNLITRFSDNQ
                     LRVAQALAVLYGVLALCFPHLRAEQFVSAAFSGSGLAPGFFLFAVPLVVSLLLGVAAG
                     LITLRRRPDVAERLRLIAFAAASPFMASGMVAPVELAPVLTAVGLLIFLVGATQFHVI
                     QGQRAQFLSRFLAPQVAELVRDQGLKSATREQTLELSVVCCDLRGFTAFSAATESKKV
                     IGILREYYDAVGAAALDVGGTIKDQAGDGVLILVGAPIRFEDHAQRALELAKRIRESG
                     VALTTRWSDGDLQLGVGVGAASGFVTVGVIGGASRLEYTAVGPAVNLASRLCSEAAHG
                     SVLVDERTTELLGTADIRAALRQCAPLSLKGFRDPVPNYALASA"
     gene            3958..4338
                     /locus_tag="VE030_12800"
     CDS             3958..4338
                     /locus_tag="VE030_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajD family HNH nuclease"
                     /protein_id="HZF24331.1"
                     /translation="MAKQPNPDQSRLDRVVAEARRHSNEREATYRERALRLFPWICGR
                     CGREFSGVRLRELTVHHKDHNHDNNPLDGSNWELLCIYCHENEHARVLDEAARAKGSG
                     ARGSVATHNPFADLQSRLDAKRKK"
     gene            4435..5634
                     /locus_tag="VE030_12805"
     CDS             4435..5634
                     /locus_tag="VE030_12805"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HZF24332.1"
                     /translation="MASWQRVRAAALAVGIALTFQTQAQQAEGPEAAAEEMQTRRTDV
                     PQSVLDGSALLAVGKAEEAAALFEQVLAAEPGNAPARYWLGEAYLALGQKNKARAAFA
                     ASLKADPNHQFAVDARIRLADPSRDAVAEPPQVFPKAGTEFQDCPKCPAMVVVAAGSF
                     VMGYPPGDSGRLYQEGPVRTVSFGKPFAVGKFEVTFDEWDACAAEKQCPKVEDKGRGR
                     GRRPAIYISWDQAVGYAKWLSNKTGKKYRLLTEAEWEYAARAGAESRYRFWGLPPDRV
                     CAFANVYDKTANKHDDSGYESLPCDDRYAEAAPAGSFKPNAFGLYDMLGNVSEWTEDC
                     LPTGLQWRGAPLDGSANLKGDCSQRAYRGGSWRENEKVYMRTPDRYKYVGARDSDLGF
                     RVARTLP"
     gene            5819..6331
                     /locus_tag="VE030_12810"
     CDS             5819..6331
                     /locus_tag="VE030_12810"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZF24333.1"
                     /translation="MMKFVLGALYALCIVSFPAAAQGTLNSYQTVDIKAPIWTVWDAV
                     KDFDGLHTWHPMFADDELKSGANDEVGAVRTLTVKDGPSFDEELLSFDPVDRKFSYRV
                     KEPSPLPISDYVSTFQVVEGRRGYTTILWRSSFRNNTDGKMKDEEVIAFIDGAYRAGL
                     DNLKATLEGR"
     gene            complement(6388..7221)
                     /locus_tag="VE030_12815"
     CDS             complement(6388..7221)
                     /locus_tag="VE030_12815"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HZF24334.1"
                     /translation="MDRRTFLHSLTAAAASSALTGRARADGLPRPAPVPGGVAIVKLG
                     PEPSPPLARFNGERVLVTGEASGWYAVVGIPLDAKVGAALPLTVERGERGAETIALEI
                     GRKRYATQHLSVKPEQVELSPEDLARHEQERAHLRGVLRIFSDQAPDSLLLLQPCEGS
                     RSDSFGKRRFFNRQPRSPHTGLDIAAPDGTPVVAAGTGNVLDVGDYFFSGHTLILDHG
                     RSFLTLYAHLSAIDAKAGDRVAAGKRIGSVGATGRVTGPHLHFSVYLNAAAVDPELFI
                     S"
     gene            complement(7362..7673)
                     /locus_tag="VE030_12820"
     CDS             complement(7362..7673)
                     /locus_tag="VE030_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24335.1"
                     /translation="MTSNEYYMKVDAAVQRAYDVLIRHNLRPQKYRIHIRAKGLSSPT
                     EPHALTISTADGSVWVTEASFPFRCLEEVDPFGSDAFASIVASRMTELIRKVRNTGRR
                     L"
     gene            complement(8012..8380)
                     /locus_tag="VE030_12825"
     CDS             complement(8012..8380)
                     /locus_tag="VE030_12825"
                     /inference="COORDINATES: protein motif:HMM:NF022591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HZF24336.1"
                     /translation="MKKLLAAFAMVALSVPALAFAQNLVTDAKLGKNVVDREITDETT
                     TFAVGEKAYLWLRVEGGTGEVLKVIWKIDDQEYPVDLRIGGSPWRTWATKTLHLAGDW
                     TVTVTDAAGNTLNEASLKVQ"
     gene            complement(8520..9677)
                     /locus_tag="VE030_12830"
     CDS             complement(8520..9677)
                     /locus_tag="VE030_12830"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZF24337.1"
                     /translation="MNSAFFALAAAAFVSGANLRLFDALLPTVAEDFAVPVTTASVVV
                     TAFTLAYGLFQIVHGPLGDRAGKLRTVGGAVLIAAVASLGCAFASSLHALTWLRFATG
                     IGAAAIVPLSLAWIGDNTPYEKRQATLGRFLGFVLLGQILGPAFGGALAEFVSWREVF
                     YVLAAVFFGVGMVLFAEDRKARRLQAPVRTQTGVLHAYADVLRDRWVRTVMSTVFLEG
                     GLFYGAFAYGGAYLKERFDLSYLIIGALLAGFGLGGLIYSLLVRRLLARLDERGFVLA
                     GGVILFVCFLTLPLLPAWQATVPIFIACGFGFYMFHNTLQTKATEMAPRARGTAIAVF
                     AFSLFMGQASGVAAFGLLISAVGYPWSFVLAGVSLVALGQWFSGRVSSHAG"
BASE COUNT         2037 a         2948 c         3182 g         1732 t
ORIGIN      
        1 tccttgaagc aatcgatgaa cccgtgatat tcaacatgaa atatcggcgc tgacgaatga
       61 acgtaaatga ggatatttga gcgcccatga atgccgctct cccgagtcac gacgtcgaga
      121 aatgcggttg tttcttccat ggtttcccgg tcgaacaact ctgctctcag aaatttactc
      181 tcgattgtta tccggtacac cataagtttg tgaggtacta caccataagt ttgcgcggta
      241 cgaaaaagtc gccagcgttt tctcttcgcg cacagggaag ggcgcacacc gttttcgcgc
      301 taaccaggcg cggggtactt gcggctgctg tgcctagtgt agagactggg ctgtgtttac
      361 ttgccgcact gagagatagt tcaaaaggac tacggattac agtcctttgt cctggggcgg
      421 cccctggccg cttcatgtgc ccggttcctg gaggacagca ggccgaagct catgggaatc
      481 gattaatatc actcatcaat gcgcgctcct ccgacctcgc cccggcggaa caggcgccga
      541 ttggctcaca tcgcacggag gtctttcgaa ccggatcccc ggctgctgcg ccggatttgc
      601 gccttgcgcg atgcccatca tgggcaatgc gcagattttc ccggaggaca tcggtatgcg
      661 ctatgagttg tattactggc ctgcaattca aggccgcgga gagttcgtcc gcctcgcgct
      721 cgaggaggcc ggcgccaatt acttggacgt ggcccgtctt gcggggcacg gcaagggcgt
      781 gccggcgctg atgcggtttc tggaaagcac cggcgtggag cgcccaccgt tcgcgccgcc
      841 gttcctcaag gcgggcgacc tggtgatcgg ccagaccgcc aacatcctgt cgtacctcgg
      901 accgcggctg ggtctgtgcc cgaaggcgga agctagccgg ttgtgggcgc atcagttgca
      961 gcttacggtc accgacttcc tggtggagat ccacgacacg catcatccga tcgcgagcag
     1021 cctgtactat cgacagcaga aaaccgaggc ccgccgcatg gcgaagcatt tcacccggga
     1081 ccggctgccg acattcctcg actatttcga gacagtgctc gcccgcaatt catcgggccg
     1141 gggtttcatg ctcggccgcg gcttgtcgta tgtcgatctg tcgatgttcc agttggtcgc
     1201 aggcctgcgt tacgcgtttc cgaagacgat ggccagggtg gagcctgacg ttcccgggct
     1261 ggctgcgctg cacgacaggg tcagcgcgcg cccgaacatt gccgcctatc tcgcgtccgg
     1321 gcggcgcatc ccgttcaacc agaacggcat ctttcgccat tacccggagc tcgatgtttg
     1381 atcgcttgtg atcgtcaacg gcgccgacac ttcaacggcg catacttgtg ataggccgct
     1441 tgccagtctt cgagaatttc ctgctgtgcc tcgcgaagcg tcacttggtt cgagcacaca
     1501 aggcactgta gcttcacctc cagccggtct ttccgtttcg cgccattctc gccctcccac
     1561 ggttgcagcg cgaggttttc gaccttgcgc gggtgcccgc cgagcgcaag cgggatgata
     1621 tggtctagtt cgtaggtctg ggcaagcgca gggttcatgt gtgcgcgctg aaaaagcatt
     1681 tgcttgacgc cgttggtgta cctggtcgcg gggcgcacgc cctttgcata gccggggaca
     1741 cagatcgtct ggtcgatggt ctcttgtctc acgacggggt tcaatgcgcc gtgatcggca
     1801 tatgctgccg atgcggcgag gaacagaagc ggcgcccaaa gtagggcatc ggtacgcatg
     1861 cttggatcga ctagtgtttc ttgggcatgg ttgcgtttgg cacttgcgca gccacgtcca
     1921 aatattcttc gagcaattcg atcccgcact cccagggaca ctcgcccgac gggtccagac
     1981 cggattctgc gagggccacc aaaattgcgg cagccgtttc acgatacgtc gccggccgaa
     2041 gctcttcgtt ctgcgtttcc gagcggatag ttacctccgc aaacgcgcta gaacctgtac
     2101 gtgatcgcat ggtttgatct ccaaatagtg agccgctaaa aaggtgcgca cggggggata
     2161 ttcccgggag agctcctgcc tataggcggc agcgtaagtc tccgaactcc ctcccggcgc
     2221 accctctata tccgcaacgg ccatgccact atggggcgtc gaaaaaatga cgcgtcgacc
     2281 actgctagca gactgtttcc gggcgctgta gagaagtgtt gaaaaactga cgaaaaggat
     2341 gtcgaacacc cagcagaacg ctgggcgcaa agcgtcacag tgaggctaaa aagtgacgca
     2401 gggaaagaga gatgatcgcg ggaaatcccg acacggctcg cgtctccgcc gacagggcgc
     2461 gcgccgggca agcggatgaa gtacggccat ttcccggagc tcgatgttta gaggccgtga
     2521 cgatagttct tacgctgatg cgagagcgta gttcggcacc gggtcgcgaa agcctttcag
     2581 actgagcggc gcacactgac gcagtgccgc gcgaatgtcc gcggtgccga gcagttcggt
     2641 ggtgcgctcg tccacgagca cactgccatg agctgcctcc gagcacagcc gcgatgcgag
     2701 attcaccgcc gggccgaccg cggtgtactc aaggcgcgag gccccgccta ttacgccgac
     2761 ggtgacgaag ccactcgccg cgccgacgcc gacgccgagt tgcagatctc cgtccgacca
     2821 gcgcgtggtg agtgccactc cgctttcgcg aatccgtttg gcaagctcca gcgcgcgttg
     2881 cgcatgatct tcgaagcgga tcggcgcccc caccaggatc aacacgccgt cgcccgcctg
     2941 gtccttgatg gtgccgccga catccagggc ggccgcaccg acggcgtcgt agtactcgcg
     3001 caggattccg atcaccttct tcgattcggt cgccgcggag aaggcggtga acccgcgcag
     3061 gtcgcagcag actaccgaaa gctccagggt ctgctcgcga gtggcgctct tcagcccttg
     3121 atcgcgcacc agttccgcca cctggggcgc gagaaaacgg gacaggaatt gcgcgcgctg
     3181 gccctggatg acatggaact gagtcgcgcc gaccaggaag atcagcagcc caacagccgt
     3241 cagcaccggc gcaagttcca ccggtgccac catgccggaa gccatgaaag gcgaggccgc
     3301 agcgaacgcg atcaggcgca agcgctcggc gacgtcagga cgccgcctga gagtaatcag
     3361 gccggcggca actccaagca acagcgaaac gacgagcggc accgcgaaca ggaagaaacc
     3421 gggcgcaagg ccactgccgg aaaaggccgc actgacaaat tgctcggccc gtagatgtgg
     3481 gaagcacagc gcgagcacgc cgtaaaggac ggcgagtgcc tgcgcgacgc gtagctggtt
     3541 gtcggaaaaa cgagtgatga gattgcgggc ggggatggtg cgccgcaccc gcagcaccca
     3601 ttcgtaggcg aagacgaagg ccaggacctc tggccacgca agaataccgc cccaccacgg
     3661 gtaggcctgg ccgcgtatca agggcagctc gatgagcgcg ctcaccacga tcgcaatggc
     3721 aaccgcggta aggaaaaacg cgagcatgcg gctggtgggc gactggcgat ccgcgacgat
     3781 gaacgcgagc gccattccga gtgcaagcag cgcggtgatg atctgcgggg tggtgtccat
     3841 ttcaggctcg cttagtgcac gcgcgcatag tacatcgacg ccggcgttcg cagggtgtcc
     3901 aaccctcgcc cgattcgtcc tgctatagtt ccatcgataa tttgcctgct ctccgacatg
     3961 gccaagcaac ccaaccccga tcagtcgcga ctcgaccgcg tggtcgccga agcgcgccgg
     4021 cacagcaacg aacgcgaggc gacttatcgc gagcgggcgc tcaggttgtt tccgtggatc
     4081 tgcgggcgat gcggtcgcga gttctcgggt gtgcgcctgc gcgagctgac ggtgcaccac
     4141 aaggaccaca accacgacaa caatccactg gatggcagca actgggaatt gctctgcatc
     4201 tattgccatg aaaacgagca cgcacgagtc ctggatgagg cggcaagggc gaagggttcc
     4261 ggggcacggg gatcggtggc cacccacaat ccgttcgccg atctgcagtc gaggctggac
     4321 gcaaagagaa agaagtgagg aacaagtggt tgggggcgaa agagaataaa cagaaccgcg
     4381 gaggagcaag acctgaaaat ttggttgcag cggcgagacc cggattcatg gtgcgtggcg
     4441 tcgtggcaga gggtgcgcgc cgcagcgctt gccgttggca tcgcgctgac atttcagacg
     4501 caggcacagc aggccgaggg cccggaagcc gctgcggagg agatgcaaac ccgccggacc
     4561 gatgtcccgc agtcagtgct cgatggctcc gctttgctgg ctgtgggcaa ggcggaagag
     4621 gcggctgcgc tgttcgagca ggtgctcgcc gccgaaccgg gcaatgcgcc cgcacgctac
     4681 tggctgggcg aggcctacct ggcgctgggg cagaaaaaca aggccagagc ggcatttgca
     4741 gcgtcgctca aggcggatcc caaccaccag ttcgcggtgg atgcgcgcat ccgtctggcc
     4801 gatccctcca gagacgcggt cgcggaaccg ccccaggtct ttcccaaggc ggggaccgaa
     4861 ttccaggact gtcccaagtg cccggcgatg gtcgtggtcg cggcgggctc attcgtcatg
     4921 ggctatccgc ccggcgactc ggggcgcctc taccaggaag gcccggtgcg taccgtgagc
     4981 ttcggcaaac cctttgcggt cggcaagttc gaggtgactt tcgatgaatg ggatgcctgc
     5041 gcggcggaga aacaatgtcc gaaagtcgag gacaagggcc ggggccgagg gcgccggccg
     5101 gcaatctata tttcctggga tcaggcagtg ggctacgcca agtggctctc gaacaagacc
     5161 ggcaagaaat atcgccttct cacggaagcc gaatgggagt acgccgcacg ggcgggagcg
     5221 gaaagcagat atcgtttctg gggcctgccg cccgaccggg tctgcgcatt tgcgaacgta
     5281 tacgacaaga ccgcaaacaa gcacgacgat tccggctacg aaagcctgcc gtgtgatgat
     5341 cgctatgcag aggctgcgcc ggccggctcg ttcaagccca atgctttcgg gctttatgac
     5401 atgttgggca acgtcagtga gtggaccgaa gactgcctcc ctacaggctt gcagtggcgc
     5461 ggcgcgccgc tcgatggcag cgccaatctg aaaggcgact gctcgcagcg cgcgtaccgc
     5521 ggcggctcgt ggcgcgaaaa cgagaaggtc tacatgcgca ccccggaccg ctacaagtac
     5581 gtcggcgccc gtgacagcga ccttgggttt cgtgttgcca gaacgttgcc gtaaagagca
     5641 aggatgaagg atgaaggatg aagggtgaag ggtgaaggat ggaacagggc gggacgccgg
     5701 gatgaaaaag gccccggttg ccgacttccc acagccgcgc ctatactggt ccggacacgg
     5761 caggagaatc caaatagccg tgacgacaac caaatcacta caagaggaga tcagggccat
     5821 gatgaaattc gtactggggg cgttgtacgc cttgtgcatc gtgagcttcc cggccgccgc
     5881 gcaaggtacg ctgaattcct atcaaaccgt tgatatcaag gcgccgatct ggacggtgtg
     5941 ggacgcagtc aaggatttcg acggcctgca cacgtggcac ccgatgttcg cggacgacga
     6001 actcaagtcc ggcgccaatg atgaagtcgg cgcggtccgc acccttacgg taaaggatgg
     6061 cccgagcttc gacgaagaac tgctgtcctt cgacccggtc gaccggaagt tcagctaccg
     6121 ggtcaaggaa ccgtcaccgc tgccgatttc cgattacgtc tccactttcc aggttgtgga
     6181 gggcagacgc ggttacacca cgatcctgtg gcgcagttcc ttccggaaca ataccgacgg
     6241 caagatgaag gacgaagaag tcatcgcctt catcgacggc gcgtaccggg ccggcctgga
     6301 caacctgaaa gctacactcg aagggcgata gacgttcaat tgccccgtcg cgcggcacgc
     6361 gacgacgggt gcttgaggct gccttcgcta cgaaatgaaa agctcaggat cgaccgcggc
     6421 cgcattcagg tagacgctga agtgcaggtg cggcccggtg actcgcccgg tggcgccgac
     6481 gctgccgatg cgtttgcctg cggcaacccg gtcgccggcc ttcgcatcga tggcgctcag
     6541 gtgcgcgtag agcgtgagga agctccggcc gtggtcgaga atcagcgtat gtccggagaa
     6601 gaagtagtcg ccgacgtcga gcacattgcc cgtgcctgcg gcgacgaccg gcgtgccatc
     6661 cggcgcggcg atgtccaagc cggtgtgcgg actgcgcggc tggcggttga agaaacgccg
     6721 cttgccgaac gaatcggagc gcgatccctc gcagggttgc agcagcagca acgaatccgg
     6781 cgcctgatcg ctgaagatcc tcaatacgcc gcgcagatgc gcgcgttcct gttcgtgccg
     6841 ggccaggtcc tcgggcgaga gttccacctg ttccggcttt acgctcagat gctgggtcgc
     6901 gtagcgcttg cgaccaatct ccagtgcgat ggtttcggcc ccgcgctcgc cccgctcgac
     6961 ggtcaggggc agcgctgcgc ctaccttcgc gtcaagagga atgcctacca ccgcgtacca
     7021 gccggaagcc tcgcccgtga ccagaacccg ctcgccgttg aaacgtgcca gcggtggtga
     7081 gggctcgggg ccgagcttca cgatcgcaac acccccaggc accggtgcgg gacgcggaag
     7141 cccgtccgcg cgcgcacggc ctgtcaacgc ggatgaagcg gcggcggcgg tcagcgagtg
     7201 cagaaaagtg cggcggtcca tgaattacgg aaggatgcgg gccgcgccat tgtaacttca
     7261 caaagcggaa aaaaatgccc tgccccaaac gatcgactcg gtaagggagg aaaccgagcg
     7321 gcgagggcag ggcgggatga agcgttgaat tctgccgcgc gtcagagtct gcggccggtg
     7381 ttccttacct tcctgatcag ttcggtcatg cgactggcca cgatgcttgc aaatgcatcc
     7441 gaaccgaacg gatcgacttc ctcgaggcaa cggaatggaa agcttgcctc agtcacccat
     7501 accgaaccgt ccgctgtcga tatggtgagt gcgtggggct cggtcgggct cgataatccc
     7561 tttgcacgaa tgtgaatccg atacttctgc ggacgcaggt tgtgtctgat gagaacgtca
     7621 taggcacgct gaaccgctgc gtccaccttc atgtagtact cgtttgacgt catggtcgta
     7681 atcctttgca acgtctctct ctccctcaat gtctttttag tacggatcgg atttgcgcgc
     7741 aagagaaaaa atgttttttt gtgtcgcgtt ctggcgacgc cgtcgctcgt tgacgacagg
     7801 tgttggaagg gcaaggatga aggatgaagg atgaaggatg aaggatgaag gcggaagtag
     7861 cggcaataaa atctgagctg agtgctcggc gatcagtgcg acgtgaattg caggtggaag
     7921 gtaaaaggaa aaaggtcaaa cggaaaagtg atgcgcactg caacggcgct gcgcaagagg
     7981 aaacgcgccg gcggcagccg gcgcgcaatt gctactgtac tttcagactg gcttcgttga
     8041 gggtgtttcc cgcggcgtcg gtgaccgtga cggtccagtc gccggcgaga tggagcgtct
     8101 tggtcgccca ggtgcgccag ggactgccgc cgatgcgcag atcgaccggg tactcctggt
     8161 cgtcgatctt ccagatcacc ttcagtactt caccggtacc gccttcgaca cggagccaca
     8221 gatacgcttt ttcgcccacc gcgaacgtag tggtctcgtc ggtgatctct cgatcgacga
     8281 cattcttgcc gagcttggcg tcggtcacca ggttttgcgc aaatgccagt gccggaacgg
     8341 acagtgcgac cattgcaaag gcagcgagaa gcttcttcat tgcagttctc ctgtttccag
     8401 ttgtgtgtcg ccgccgggcg acacccggcg gcccaaaggg aaggtcgtgc cgttcccttc
     8461 atgacgacgg cgaatgatac ttcgatattt gcttttccgc gcgggcggat tggtcagggt
     8521 cagccggcgt gagaactgac ccggccggaa aaccactggc cgagcgccac caagctgaca
     8581 ccggccagca cgaacgacca cgggtagccg acagcgctga tgagcaggcc gaacgccgcg
     8641 acaccgctcg cctggcccat gaacaggctg aacgcgaaaa ccgcgattgc cgtgccgcgc
     8701 gcccgcggcg ccatctcggt ggccttggtc tgcagcgtgt tgtgaaacat gtagaagccg
     8761 aatccgcagg cgatgaagat tggaaccgtc gcctgccacg cgggaagcag cggcagcgtg
     8821 aggaagcaaa cgaacaggat cacaccgccg gccaggacga aacctctttc gtcgagccgc
     8881 gccagcaacc ggcgcaccag caggctgtag atcaggccgc cgagaccgaa gcccgccagc
     8941 agcgcgccga tgatcagata ggacagatcg aaacgttcct tcagatacgc gccgccgtag
     9001 gcgaatgcgc cgtagaacag gccgccttcc aggaacaccg tcgacatcac ggtgcgcacc
     9061 cagcggtcgc gcaacacgtc ggcgtaggcg tgcagcactc cggtttgcgt gcgtactgga
     9121 gcctgcaggc ggcgcgcctt gcggtcctcg gcgaacagca ccatgccgac gccgaaaaac
     9181 acggcggcga gcacgtagaa cacttcgcgc cagctcacga actccgccag cgcgccgccg
     9241 aatgcgggac cgaggatctg tccaagcagc acgaatccga gaaaacggcc gagcgtggcc
     9301 tggcgctttt cgtatggcgt gttgtcgccg atccaggcga gcgacagcgg cacgatggcg
     9361 gcggcgccga tcccggtggc gaaacgcagc cacgtgagcg catgcaggga cgatgcaaag
     9421 gcgcagccga gcgaagccac cgcggcgatc agcacggcgc cgccgactgt cctgagcttg
     9481 ccggcgcggt cgccgagcgg gccgtggacg atctgaaaca atccataggc gagcgtgaac
     9541 gcggtcacca cgaccgaagc ggtggttacc ggaaccgcga agtcctcggc gacggtcggc
     9601 agcagcgcgt cgaagagccg caggttggcg ccggagacga acgcggcggc ggccagtgcg
     9661 aaaaaggcgg agttcatggc gggtgaaaga ctgcgattct gcgcgggaac ggtggcaggt
     9721 tatcccagag gcgggacaaa aaacagggtt gtccggacat tcatgtgggt gatcggcgcc
     9781 cgggtgttga agaattgaac ggttggcgga acgttcgtcg ccaaaccctt acggccaagc
     9841 gttaaccgca agtgacgcca aggacgcaag gtaaagacaa tagtgtaaag gcgaggctg
//
LOCUS       DASQOP010000329         8599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_371146, whole genome shotgun sequence.
ACCESSION   DASQOP010000329 DASQOP010000000
VERSION     DASQOP010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8599
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371146"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..1151)
                     /gene="pqqE"
                     /locus_tag="VE030_12835"
     CDS             complement(6..1151)
                     /gene="pqqE"
                     /locus_tag="VE030_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HZF24338.1"
                     /translation="MEKAGSVPNKPGNPLWLLAEITYKCPLHCVFCYNPVDYTTYGQE
                     LSTEDWLRVLRQGREIGATQLGFSGGEPLVRDDLEIMVAEARKLGYYSNLITSGVGLN
                     EKRIGAFKEGGLDHIQLSFQDSTKELNDFLSSTRTFDLKQKVAKLIKKHDYPMVLNCV
                     LHRHNIDHVQQILEMAEAMEAEYVELANTQYYGWAYVNRNQLLPTRAQLQRAEEVTNK
                     FRQRVGNKMRIFFVVPDYYENRPKACMNGWGSVFLTITADGLALPCHEARQLPGLTFP
                     NVRDHDLRSIWYDSPGFNAFRGDAWMKEPCRSCPEKVKDFGGCRCQAYMLTGDATNAD
                     PVCDLSPHHHLVTDAVDKAQKPAPAVEVKPILFRDDKNSRALSKTPA"
     gene            complement(1114..1407)
                     /gene="pqqD"
                     /locus_tag="VE030_12840"
     CDS             complement(1114..1407)
                     /gene="pqqD"
                     /locus_tag="VE030_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HZF24339.1"
                     /translation="MSDTGTFTDETVFELQRHFRFQWEPAQQGFVLLYPEGMVKLPGS
                     AGEIMKRIDGKSSVRAIVEDLQNTFPGAALRGDVIGFLEHVHGKGWIRPKQTG"
     gene            complement(1400..2119)
                     /gene="pqqC"
                     /locus_tag="VE030_12845"
     CDS             complement(1400..2119)
                     /gene="pqqC"
                     /locus_tag="VE030_12845"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HZF24340.1"
                     /translation="MGAAENLPWSKEEFESQLRAKGNRYHIHHPFHIAMNSGKFTREQ
                     VQGWVYNRFYYQISIPIKDAAVLSNMTDREQRRKWIQRIIDHDGVEGQEGGIEAWLRL
                     GEACGLKRQEITSLGHVLPGVRFAVDAYVNFARRAPWQEAACSSLTELFAPEIHKKRL
                     ENWPQHYPWIEPEGYSYFRKRLSEARRDVEHGLKVTLDYFKTREEQERALGILQFKLD
                     VLWSMLDTMQINYGIGETRYV"
     gene            complement(2132..3046)
                     /gene="pqqB"
                     /locus_tag="VE030_12850"
     CDS             complement(2132..3046)
                     /gene="pqqB"
                     /locus_tag="VE030_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HZF24341.1"
                     /translation="MRIRILGAAAGGGFPQWNCNCRLCNGVRKGTVRATGRTQSSIAV
                     SADNINWVLFNASPDLLAQLKAFPELQPARAIRDTAIRSIVLMDAQIDHTTGLLMLRE
                     GRPLEIYCTGMAREDLTTGNPLFNILEHYCGVHWHQVPTKAGNEFPVIGAEELTFTAV
                     PLSSKAPPYSPHRDNPHEGDNIGMRVSDKRSGKVLFYAPGLGAIEPHLRPFLEEADCL
                     LVDGTFWTDDEMIKLGVSKKHARDIGHLPQSGTGGMIDVLKPLKASRKILIHINNTNP
                     ILDEDSRERKELDSRGIEVAYDGMDVTL"
     gene            complement(3126..3197)
                     /gene="pqqA"
                     /locus_tag="VE030_12855"
     CDS             complement(3126..3197)
                     /gene="pqqA"
                     /locus_tag="VE030_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HZF24342.1"
                     /translation="MWSKPEYTEMRFGFEVTMYIANR"
     gene            3432..4418
                     /locus_tag="VE030_12860"
     CDS             3432..4418
                     /locus_tag="VE030_12860"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HZF24343.1"
                     /translation="MSDAVFDRGLYFPSPMTDWYRGRPFKVALVGSLLIHAALIALIP
                     GFRAVVIEPPKVLNVEIVLPEQPLVRPMTLREVVPPAAPQRTVVEELVRPEPRIEPRP
                     EPVMRQPEPEAPPIPRAEIAPPPRPEPLPEPVAVPKPEVWPEPRIEPKIEIKPEPRVE
                     PPPVERVEPLPEPRTEVRPEPQSVQRVEPTLPPVAAAPVQPAPVAVPVPPPVPPRPAA
                     LKKEPFVLNVEPRLLQMFGQSISKEIKRYQKYPPLAQRRGWEGTAEVLLQIAADGKVS
                     SITLGKSSGRSVLDDEALNMVRRASPLPQAPQNLRGRELTVTVPIVFKLQES"
     gene            4442..5419
                     /gene="pip"
                     /locus_tag="VE030_12865"
     CDS             4442..5419
                     /gene="pip"
                     /locus_tag="VE030_12865"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HZF24344.1"
                     /translation="MDQNQASGEVLYPAIEPYRKDRLDVGAGHRVYFEESGNPAGFPV
                     LFIHGGPGSRTRPAHRRFFDPAFYRIVLFDQRGCGQSTPAGSTLENTTPHLVADIEQL
                     RHHLGVDRWLLFGGSWGSTLSLAYAIAHADRVAGMVLRGVFLGSTAEVEWYVSGIRRF
                     VPEAWADFAGDAGESIIEHYRQLIDHPDASGALAAARRWCDYEARIMAIGEMPNGNDG
                     GRNAATEEEMLASARIQLHFLAHHCFLRPNQLLDDLWRIGNKPIILVQGRMDMVCPAI
                     SAFEVSRRLAGADLRLVPNGGHSAMQPAIAEELCAATARMCDVLGGSRR"
     gene            5416..7179
                     /locus_tag="VE030_12870"
     CDS             5416..7179
                     /locus_tag="VE030_12870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZF24345.1"
                     /translation="MRKFGLRFRINFVITLVIVIFALAASDVLIEETRRSIREEIEAG
                     TKVTVQLMETVIANTRANAQSGPYNQALLSFLRHLGRVRANEIKMYDENDQLIYTSPP
                     SHYKQGRWAPDWFNRLADPKIGEFRLNIPNGTVVVRPDASRSILDAWDDLKNFFWLVL
                     GFFVLVNALVFWLLGRSLRPIGKILGGLSEMERGRFDTRLPKFELPEFDAISHTFNRM
                     AENLEQSLAENQRLALVTKQSSDAIIIHDLGGRISFWNPAAGRMFGYRDDEIVGRSAT
                     LLAPPERRGEIQENLDAVTQRQLVEMVETQRLTKDGRVVDVALSAAPLVDPVSNEVIG
                     EICSMRDITEHKLAQRTERELEQNRKLTQLIQNRLEEERRSIARELHDELGQCVTAIK
                     TIGTAISNRTRESAPEIHGNAQTIVSVATHIYDVVHGIIRQLRPTALDHLGLSATLKE
                     TVAAWRDRHKDIACELRLEGELEGLGETVNITIYRIVQECLTNVIRHAAATRADILVA
                     RERDSGRGDVVRVIVRDNGKGIALRDESEATRFGLMGMRERVQALDGVFQIDNRPGEG
                     VTVTAAIPAKATVATDGVEAA"
     gene            7194..7850
                     /locus_tag="VE030_12875"
     CDS             7194..7850
                     /locus_tag="VE030_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZF24346.1"
                     /translation="MNSKAAGGGIGVMLVDDHAVVRMGFRLLLEGSADIKVVAEADSG
                     EEACRKFPEVRPDVVVMDISMPGIGGLEAIDRILAREPAARILVLSAHEDAMHARRVL
                     KAGAAGYLTKRSAAEALMQAIRQVYQGKTFLEPEIAQQMAVQQVNGDRNPLDMLSEKE
                     FKVFLALAKGQSVAEIAEVMSLSPRTIGTHLYNIKQKLGASNSAELAIIAIRAGLIEP
                     "
     gene            8033..8104
                     /gene="pqqA"
                     /locus_tag="VE030_12880"
     CDS             8033..8104
                     /gene="pqqA"
                     /locus_tag="VE030_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HZF24347.1"
                     /translation="MWTKPEYTEMRFGFEVTMYVFNR"
     gene            8302..8580
                     /locus_tag="VE030_12885"
     CDS             8302..8580
                     /locus_tag="VE030_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24348.1"
                     /translation="MSLSTALEARWVDKGFTNDLNDQSADAYTVVNWRPGFEQKFGGL
                     RQTEFARVDNIFDENYVGSVIVNEVNGRYYEPAPGTNYTVGLAASYQF"
BASE COUNT         1703 a         2498 c         2677 g         1721 t
ORIGIN      
        1 ttctatcagg ccggcgtttt gctcaaggcg cgcgaattct tgtcgtcgcg gaacaggatc
       61 ggcttgacct cgactgcggg cgccggtttc tgcgccttgt ctactgcgtc ggtcaccaga
      121 tgatgatgag gagacaagtc acaaaccggg tcggcgttgg tggcatcgcc ggtaagcatg
      181 taggcctggc agcggcagcc gccgaaatcc ttgacctttt ccggacagct tcggcacggc
      241 tccttcatcc acgcatcgcc acggaaggcg ttgaagccgg gcgagtcgta ccagatcgag
      301 cgcaggtcgt ggtcgcgcac gttggggaag gtgaggccgg gtagctggcg tgcttcgtgg
      361 caaggcagcg ccaggccatc ggcggtgatg gtgaggaaaa ccgagcccca gccgttcatg
      421 catgctttcg ggcggttttc gtagtaatcg ggaaccacga agaaaatccg catcttgttg
      481 cctacgcgtt ggcggaattt gttggtgact tcctcggccc gctgcaattg tgcgcgggtc
      541 ggcagcaatt gattccggtt cacgtaggcc cagccgtagt actgggtatt ggcgagttcc
      601 acgtactccg cttccatcgc ctcggccatc tccaggatct gctgcacatg gtcgatgttg
      661 tgccggtgca gcacgcagtt gagtaccatg gggtagtcgt gttttttgat cagcttcgcc
      721 actttctgct tgagatcgaa agtgcgtgta ctcgacagaa agtcgttcag ttccttggtc
      781 gagtcctgaa aggagagctg gatgtgatcc aggccgcctt ccttgaacgc gccgatgcgt
      841 ttttcgttga gccccacgcc ggaagtgatc aggttggagt aatagccgag cttgcgcgct
      901 tccgcgacca tgatctcgag gtcgtcgcgc accagcggct cgccgccgga aaaccccaat
      961 tgcgttgcgc cgatttcgcg gccctggcgc agcacccgca accaatcctc ggtcgatagc
     1021 tcctggccgt aggtcgtgta gtccaccggg ttgtagcaga acacgcagtg caacgggcac
     1081 ttgtacgtga tctccgccag cagccagagc gggttacccg gtttgtttgg gacggatcca
     1141 gccttttcca tgtacgtgct ccagaaagcc gatgacgtcc cctcgcaggg ccgcacccgg
     1201 aaatgtgttt tgcaaatcct cgacgattgc gcgcacgctg gatttgccgt cgattcgctt
     1261 catgatttct ccggcgctgc ccgggagctt taccatgcct tccggataga gcaggacgaa
     1321 accctgttgt gccggctccc attggaagcg gaaatggcgc tgaagttcga aaacagtttc
     1381 gtccgtgaat gtcccggtat cagacatacc gggtttcgcc gatcccgtag ttgatctgca
     1441 tcgtatcgag catgctccac agcacgtcaa gcttgaactg caggattccg agcgcccttt
     1501 cctgctcttc gcgggtcttg aaataatcga gcgtgacttt gagtccgtgc tcgacgtcgc
     1561 ggcgtgcttc cgacaggcgc ttgcggaagt aggaatagcc ctcgggttcg atccacggat
     1621 agtgctgcgg ccagttctcc aggcgcttct tgtggatctc gggcgcgaac aattccgtca
     1681 gcgacgaaca ggcggcctcc tgccatggcg cgcgccgggc gaagttgacg taggcatcca
     1741 ccgcgaagcg cacgccgggc agcacgtgcc cgagcgaagt aatctcttgg cgtttcaacc
     1801 cgcatgcctc gcccagccgc agccacgcct cgatgccgcc ctcctgtccc tcgacgccgt
     1861 cgtggtcgat gatgcgctgg atccacttgc ggcgctgttc gcggtccgtc atgttggaga
     1921 gaaccgctgc gtccttgatc gggatgctga tctggtagta gaagcggtta tagacccagc
     1981 cttgcacctg ctcgcgggtg aacttgccgc tgttcatcgc gatgtggaac gggtgatgga
     2041 tatggtagcg gttgcccttg gcgcgcaact ggctctcgaa ttcctctttg ctccacggca
     2101 gattttcggc tgcgcccatg cgcgctcctg ttcagagtgt tacgtccatt ccgtcgtacg
     2161 cgacctcgat gccgcgggag tccagttcct tgcgctccct cgagtcttcg tcgaggatgg
     2221 gattggtgtt gttgatatgg atcagtatct tgcgcgacgc cttcagcggt ttcagcacgt
     2281 cgatcatgcc cccggtaccc gactgcggca ggtggccgat atcgcgtgca tgctttttcg
     2341 agacaccgag tttgatcatc tcgtcgtcgg tccagaacgt gccgtccacc aacaagcagt
     2401 cggcttcctc gaggaagggt ctcaggtgcg gctcgattgc gccgagccca ggggcgtaaa
     2461 acagcacttt gccggagcgc ttgtcgctga cgcgcatacc aatgttgtcg ccctcgtgcg
     2521 ggttgtcccg gtgcggcgaa tagggcggcg ccttgctgct cagcggtacg gcagtaaaag
     2581 tgagttcttc cgcgccgatc accggaaact cgttgccggc cttggtgggc acctggtgcc
     2641 agtgtacgcc gcaatagtgt tcgaggatgt tgaatagcgg gttgcctgtg gtcaggtcct
     2701 cgcgcgccat gccggtacag tagatctcca gcggcctgcc ttcacgcagc atcagcaatc
     2761 cggtggtatg gtcgatctgc gcgtccatca gcacgatcga gcggatcgcg gtgtcccgga
     2821 tcgcccgcgc cggctgcaat tccgggaacg ccttgagttg ggccaacagg tctggcgaag
     2881 cgttgaacaa cacccagttg atgttgtcgg cgctgacggc gatggaggac tgggtgcggc
     2941 ccgtggcccg gacggttccc ttgcgcacgc cgttgcatag gcggcagttg cagttccact
     3001 gcggaaatcc tccgccggcc gcggcaccga gaatccttat tcgcatcgtc cgtcaaaata
     3061 atagtttatg gcctgcataa aaacgaaagc cccgcgaagg ggctttcgtc caagctcaac
     3121 ccggttcagc ggttggcaat gtacatcgtg acttcaaagc caaaacgcat ttcggtgtac
     3181 tcaggcttgc tccacatagt cttctccagt tgaggttgaa atttcgcggg gatggcccgc
     3241 tcgttgccga atcatccgac gctggttgcg ccacgcattg ctgcgcgaaa cctgcatccg
     3301 atgagtccgc gagcattgtg cgcggagggg gtgcaaactc gcacacgaaa atcaccaatt
     3361 gattctcatg attatcatga gttttgctcg gaaactttac ttacctcaag gccttgcact
     3421 acgcttccgg catgtctgac gccgtattcg acagagggct ttattttccc tcgccaatga
     3481 ccgattggta ccgcgggcgg cctttcaagg tcgccttggt gggctcgctg ctcatccacg
     3541 cggcgctgat tgcgctcatc cccggttttc gtgcggtggt gatcgagccg cccaaggtgc
     3601 tcaacgtcga gatcgtcctt cccgagcagc cgctggtgag accaatgaca ttgcgggaag
     3661 tagtgccgcc ggcagcgccg cagcgaacgg tcgtggaaga gttggtgcgg ccggaaccgc
     3721 ggatcgagcc gcgccctgag cctgtcatgc ggcagccgga gcccgaggca cccccgattc
     3781 cccgcgcgga gatcgcgccg ccgccccgtc ccgagccgct tcccgagccc gtggcggtgc
     3841 caaagccgga agtgtggccg gagccgcgta tcgagcccaa aatcgaaatc aaacccgaac
     3901 ccagggtcga gccgccgccg gttgagcgcg tggaaccgct gcccgaaccc cggacggaag
     3961 tgcggcccga gccgcaatcg gttcagaggg tggagccgac ccttccgcct gtcgcagcgg
     4021 caccggtcca gcctgcgccg gtcgccgtgc cggtgccacc ccctgtacct ccgcggccgg
     4081 cggcgctgaa gaaggagccg tttgtgctga acgtggagcc gcgtctgttg cagatgttcg
     4141 ggcagtcgat ctccaaggaa atcaagcgtt atcagaaata tccgccgctg gcgcaacgcc
     4201 gcggctggga gggaacggcg gaagtgcttc tgcagatcgc cgcggatggc aaagtttcta
     4261 gcatcacgct gggcaagagc agcgggcgat cggtgctcga cgacgaagcg ctcaacatgg
     4321 tgcgcagggc ttctcccctg ccgcaggcgc cgcagaacct gcgcggccgc gaactgacag
     4381 tgaccgtgcc gatcgtcttt aaactgcaag aatcctgatt ctccgttcgc caggcatggt
     4441 cgtggatcag aaccaagcca gcggcgaagt cctttatcct gcaatcgaac cctaccgcaa
     4501 ggatcggctt gatgtcggtg ccggtcatcg cgtctatttc gaggaaagcg gcaatccggc
     4561 gggatttccc gtgttgttca tccacggcgg cccgggaagc aggacgcgcc cggcgcatcg
     4621 ccgtttcttc gaccccgcct tctaccgcat tgtgctgttc gatcagcgtg gttgcgggca
     4681 gagcacgccg gcaggttcga ctttagaaaa cacgaccccg cacctggtgg ccgacatcga
     4741 gcaattgcgc catcatctgg gcgtagatcg atggctgctg ttcggaggct cctggggaag
     4801 tacgttgagc ttggcctacg caatcgcaca cgcggatcgg gttgccggga tggtgttgcg
     4861 cggcgtattt ctcggcagca cggcggaagt cgagtggtat gtatcgggaa ttcggcgctt
     4921 cgtccccgag gcctgggcgg actttgccgg agatgccggc gagtccatca tcgagcacta
     4981 tcggcaactc atcgatcacc ccgatgcatc gggcgcgctg gcggcggccc ggcgctggtg
     5041 tgactatgaa gccaggatca tggcgatcgg ggagatgccc aatggcaatg acggcggccg
     5101 caacgccgcg actgaagagg aaatgctggc ttccgccaga atccagctcc attttctggc
     5161 ccaccactgt tttctgcgac cgaaccagtt gcttgatgat ctctggcgca tcggcaataa
     5221 accgataatc ctggtacagg gcagaatgga tatggtatgc ccagcaataa gtgcatttga
     5281 ggtgtcgcgg cgactggccg gtgcggacct gcgtctggta ccaaatggcg gccactctgc
     5341 aatgcagccc gcgatcgccg aggaactgtg cgcagcgacg gcacgtatgt gcgatgttct
     5401 gggcgggtcc cggcgatgag aaaattcggc ctgcgcttcc ggattaattt cgtgattacg
     5461 ctggtcatcg tgattttcgc gctggccgca tccgacgttc tgatcgagga aacgcgtcgc
     5521 tccatccggg aagagatcga agcaggcacc aaggtcaccg tgcaattgat ggaaactgtg
     5581 atcgccaaca cgcgggccaa cgcgcaaagc ggtccgtaca atcaggcgtt gctgtcgttc
     5641 ctgcgccacc tcggtcgcgt acgtgccaac gaaatcaaga tgtacgacga gaatgaccag
     5701 ttgatctaca cctcgccgcc atcccactac aaacagggca ggtgggcacc ggactggttc
     5761 aaccggctgg cggatcctaa gatcggcgag ttccggctga atatcccgaa tggaacggtg
     5821 gtcgtcaggc cggatgcttc gcgatcaatt ctcgatgcat gggacgatct caagaatttc
     5881 ttctggctgg tgctgggatt cttcgtgctg gtcaacgcgc tggtgttctg gttactgggc
     5941 cggtcgctgc ggcccatcgg caaaattctc ggcggtttgt cggaaatgga gagaggccgc
     6001 ttcgacacgc gcttgccgaa attcgaactt ccggaattcg acgcgatcag ccataccttc
     6061 aaccgcatgg cggagaatct cgagcaaagc cttgcggaaa accagcggct cgcgctggtc
     6121 accaagcaat ccagcgacgc catcattatc catgacctcg gaggccgcat ttcgttctgg
     6181 aacccggcgg cgggccgcat gttcggctat cgcgacgatg aaattgtcgg gcgttccgcc
     6241 acattgctcg ccccgccgga acgtcggggc gaaatccagg aaaacctcga tgcggtcaca
     6301 cagcgccaac ttgtcgaaat ggtggagacc cagcgcctga ccaaggacgg cagggtggtg
     6361 gatgtcgcgc tgtcggcggc acccctggtg gacccggtga gcaacgaagt gatcggtgaa
     6421 atttgcagca tgcgcgacat aaccgagcac aaattggcac agcggaccga gcgcgagctg
     6481 gagcagaacc gcaaactcac gcagctgatc cagaaccggc tggaagaaga gcgccgctcg
     6541 atcgcgcgcg aactgcacga cgaactcggc cagtgcgtaa ccgcaatcaa gaccatcggt
     6601 acggcgatct ccaaccgcac ccgcgaaagc gcgcccgaga tccatggcaa tgcgcagacc
     6661 atcgtatccg tcgccacgca catttacgat gtcgtgcacg ggatcatccg tcagctgcgg
     6721 cccaccgcgc tggatcacct gggcctgagc gcgacgctga aggagaccgt ggccgcgtgg
     6781 cgggacaggc acaaggacat cgcctgtgag ctcaggctcg agggcgagct ggagggattg
     6841 ggcgaaaccg tcaacatcac gatctaccgc atcgtccagg aatgcctgac caacgtaatc
     6901 cgccatgccg cggcgacgcg cgcggacatc ctggtcgcgc gcgaacgcga ttccggccgc
     6961 ggcgacgtcg taagggtcat agtgcgcgac aacggcaagg gcattgccct gcgcgatgag
     7021 agcgaagcca cgcgcttcgg tctgatgggc atgcgcgagc gcgtccaggc cctcgatggg
     7081 gtgtttcaga tcgacaaccg gccgggcgag ggcgtaacgg ttaccgccgc aatcccggca
     7141 aaggccaccg tggcgaccga cggcgtggaa gcggcatagc gggaggagtc aggttgaaca
     7201 gcaaagcagc gggcggtgga atcggtgtaa tgctggtcga tgatcatgcg gtcgtgcgca
     7261 tgggattcag gctactgctg gagggatcgg ccgacatcaa agtggtcgcc gaggccgata
     7321 gcggcgagga agcgtgtcgc aagtttccgg aagtcaggcc ggatgtcgtg gtcatggata
     7381 tatcgatgcc ggggatcggc ggcctggagg cgattgatcg catcctggcg cgtgagccgg
     7441 cagcgcgcat tctggtgttg tccgcacacg aggatgcgat gcacgcgcgc cgtgttctga
     7501 aagcgggcgc cgccggttac ctaaccaaac gcagtgcagc agaagccttg atgcaggcga
     7561 tccggcaggt gtaccagggc aagacgttcc tggaaccgga aatcgcccag cagatggcgg
     7621 tgcagcaggt caatggcgat cgcaatcctc tcgacatgtt gagcgaaaag gaattcaagg
     7681 tattcctggc gctggcgaaa ggacagtcgg tggccgagat cgcggaagtc atgtctttga
     7741 gcccccgcac catcggcact cacctttaca acatcaaaca gaagctgggg gcgtcgaatt
     7801 ctgccgaact ggccatcatc gcgattcgcg ctggtcttat agagccatga ttattaatga
     7861 gaatactgaa aaaggcggct ctctgaaaat ctaatgaaaa tcatgagccg gatttcagtg
     7921 ttttcctgta gggcggggta atggggctgc aacagaatac gatccaaggt cgtgaggaag
     7981 cggccgtgac ccccccgata gaagtggatt aactctctga aggagcctca ccatgtggac
     8041 caaacccgaa tacacagaaa tgcgctttgg ctttgaagtc acgatgtacg tcttcaatcg
     8101 ctgatagcac tgcaagtccc gaagggctgc tttcgagcag cctttttttt tgcgtcaagg
     8161 cggcgcggga gcgccccaag aaaaaagaga aaactggatc aaaaaaaatc aagggagaac
     8221 gtgccgtgcc tcgcaatttc aggaagatcc cagataccaa gcaacatgga ttatggcgaa
     8281 cttggctggc ggtacgcacc gttgagtctg tctaccgcat tggaggcacg ctgggtggac
     8341 aaggggttca ccaacgacct gaacgaccag tcggcagatg cgtataccgt ggtgaactgg
     8401 cgtccgggct ttgaacagaa attcggcgga ctacgccaga ctgaattcgc gcgagtcgac
     8461 aacatttttg acgagaacta tgtgggatca gtaatcgtca acgaggtaaa cggccgctac
     8521 tacgaacctg cacccgggac gaactatacc gtggggcttg cggcgagtta tcagttctga
     8581 aaagagccgt agggagcaa
//
LOCUS       DASQOP010000330         9720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1178530, whole genome shotgun sequence.
ACCESSION   DASQOP010000330 DASQOP010000000
VERSION     DASQOP010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9720
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178530"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..3163)
                     /locus_tag="VE030_12890"
     CDS             complement(131..3163)
                     /locus_tag="VE030_12890"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZF24349.1"
                     /translation="MSQQFRILMVEDAASDAELMVRELKRAGVRCDAHRVEAAVEYRR
                     ELEEFRPHVILSDFSMPKFDGMEALRIARQSHPDIPFIFVSGTLGEEYAIRALKSGAT
                     DFVLKSNLLRLPSSVERAIKEAEERRARLVLEQQVHESEERFRQLAENMHEVFWVHAV
                     DADQSLYISPAYERVWGSSRGDVGKVHRDWLESIHPEDRTQVRKSLEEMTAGAKALDV
                     EYRIVRPDGSLRWIHDRGFPIRDAQGKIYRAAGIAEDITERMQLKVALQEQAAGLQRA
                     QALAKLAHVITGPGGSFVRWSETLPQLIGVAPAAMPQGTRAWLDILHVDDREKFRSKS
                     IEAAKTGARVDVDYRLWRGDGELIHVRQVIEPLEGRSGNQDGLMWFCTIQDVTEQTRT
                     EERIKRLNRVFAVLSGINALIVRIRDREELFQEACRIAVDRGGFTIGWISVLDPASGK
                     LVPVANSGLPLDSAGGGATLDRSAGFVAAGTAEVALREQRPAFDNEIEREPGTANGAL
                     APDTLSIRRAAIQMGAKSVIVLPLYVEGKTFGILTLYAPERNFFDDDEIKLLTELAGD
                     ISFALEFIAKEEKVDYLAYYDPLTGLPNRALFSERLGQQLRTRNDSPELVGVALMDVE
                     RLRMVNDTLSRQAGDELLRVIARRLESNATITRFSNPARVVGDSFGVIIRGARDANEL
                     AQLVEAGMKACFGMPFVINGQELRIDARAGVSAYPTDGFSAESLLHNAEAALRRAKQA
                     KERFVFYAPEMTARVAEILVLETRLRKAIELEQFVLHYQPKFDVATGTISGVEALIRW
                     NDPETGMVPPLKFIQILEETGLIFEVGRWALRQALADQQRWRAKGLPVPRVAVNVSAQ
                     QLRDPHFVQDVQLALASQEGLRPSLELEITESLLMEDIERNIERLKMLRDLGVTIAID
                     DFGTGYSSLNYLARLPMDTLKVDRSFVVGMAQSSESMTIVSTIISLAHSLGLKVVAEG
                     VETEEQLNLLRQLKCDGIQGFLLSRPVASAEIANFLGR"
     gene            complement(3451..4701)
                     /locus_tag="VE030_12895"
     CDS             complement(3451..4701)
                     /locus_tag="VE030_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZF24350.1"
                     /translation="MATPRDESSLSKLLSGNPRALGGSLDQLLPDFLRAVRSHLGMDV
                     AFLSEFTDGQRVFRHVDSSLENQPVKVGGADPLEASYCQRVVDGRLPELIQDATAVPA
                     AMELPVTRALPVGAHLSVPIKLKDGSVYGTFCCFSFTPDLSLNQRDLGMMRVFADLAA
                     DVIERNLEKGKQRDEKAKRIQSVISGDGLSIVYQPIINVAQDKVVGFESLTRFSATPL
                     RGPDVWFNEAASVGMGAQLEMRAVELAMAALDSLPQDVYLSFNVSPEALINGDLRRVL
                     EGMLLERLVLEITEHATIDEYSELTNALAPLRTQGLRLAVDDARAGFASFRHILRLQP
                     DIIKLDMSLTRDIDTDAARRALASALIRFAHETGSKIVAEGVETASELRVLRSLGVNK
                     AQGYFLGRPAPLANALQSSQATRN"
     gene            complement(4931..5254)
                     /locus_tag="VE030_12900"
     CDS             complement(4931..5254)
                     /locus_tag="VE030_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24351.1"
                     /translation="MASTLSITHYTSHEPLGIVSDEEYQRFKDQLLEAFQDEWPDAIV
                     SIEDDEEPYTEVDGVRGQAEQDVRDRIEDIVTEVIDSGDWRDEEEDPYDEEEELDDEE
                     EEDNF"
     gene            complement(5414..6553)
                     /locus_tag="VE030_12905"
     CDS             complement(5414..6553)
                     /locus_tag="VE030_12905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HZF24352.1"
                     /translation="MQAINIEDLRSRARRRLPKVIFEFIDGGAQDETTLRANREDFQK
                     WRFRTRVLTDVSRRDQSITLFGQRCASPLVLAPTGLAGLLSRRGELAAARAAEKYGVP
                     YCLSTMATCSIEEITTETTQPKWFQLYVLRDRGLTKEFIERARASRCTALVLTVDTKV
                     QGPRERDMRNGFTVPPRFTIATILDFARHFSWLFDVGLGPRIAFRNFEGTKAAATDAV
                     TITEFIAGQYDLAVNWRDVEWFKSAWGGPVLLKGILSVEDAKLAVAHGADGVIVSNHG
                     GRQLEGALSAVHALPAIADAVGDRIEVVLDGGIRRGADVVRARALGAKACMIGRAWLY
                     GLASAGQAGVERALEILRDEIDITLTLLGRPTLAEIDRDVLERVG"
     gene            complement(6658..8205)
                     /locus_tag="VE030_12910"
     CDS             complement(6658..8205)
                     /locus_tag="VE030_12910"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HZF24353.1"
                     /translation="MTSLSFMTGAIVTQPGIPMRGKHAPEAVGIRLAGWQRAISGNIR
                     CKTMEEQVNLALRLAAALFGCAYACIGAAQQPHIVYILADDLGWKDVGYHGSPIRTPH
                     LDKLAQGGARLEKFYTLPYSTQTRAALMTGRYPMRYGLQTLSILPWSRYGLPADERML
                     PQALKEAGYRTAMLGKWQLGHFKKEFWPTRRGFDSFYGSFNGEIDYFRKTDRIGEADW
                     HRNERQVKEAGYVTTLLGREAASLISGHDATTPLFLYLSFNAPQMPLQAPREYLEMYR
                     DIADETRRAYVAMVTALDDAIGALTSALESKGMAANTLIVFHSDNGGALPNKYATGDG
                     DVSKPAADNGPFQDGKGGLHEGSMRVAAFAAWPGRIQPGVVAERIHVTDMYPTLLRVA
                     GAKLEQKRRVDGIDQWATIADGKPSARKEMLLAMEDSRGALLIENWKLIVYSRLPVRY
                     ELFNIQDDPSEEDNRAEREPQRVQEMLARFNEYAWEMAPSLYLEDLLKPRKYDAPMYW
                     GENPVRP"
     gene            8186..>9720
                     /locus_tag="VE030_12915"
     CDS             8186..>9720
                     /locus_tag="VE030_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24354.1"
                     /translation="MKDSEVILSVLGSIERRLWLRRALQEITYGACVVLFSLIAFQFV
                     GVALSSAIPAVGTGARTAVVAAVVMLGAYIAWRALRQVTLAQAAGEADARARLKDELK
                     SAYWFVSMPEPSPFAELQIARAAATARRLDPGAIVPGRPPRSLYAVAGLGAALLLVTW
                     IAPQQSHSWDGADAATAAAGETQDLRALLEGAPQDPEIEKLDRALEELQRADATAEEK
                     ARAAAQARDAIEQANMEAAAAREGLVRLAGAMRANPRFEKAAEALRAGRNDEALALLR
                     QLRAEVGGRDTRALEKDGIRPLSDSEKGLDRGIEERLAEGSRDLSSINARVDQDALDR
                     VVNALQEANQRLEVQSRVIEVKRRMQEAMIPTSQRSALTASQFDSSDNAPNATAAPDT
                     GNADIRGGAMFRQGAVTRRNDDEPARDGNMSGSAAGNSEALALEGAATRRLDAQLKLE
                     AIHRRDDADDDEKADQGWFYSASQQQKSLLGFEDVRARSSFDRENAMSVERVPIRQKH
                     AVR"
BASE COUNT         1747 a         2995 c         3011 g         1967 t
ORIGIN      
        1 ggtctggcag accggatgga tgtgattgtc ggaatgttgc ggtttctgga ggaggaacag
       61 ccgaagcact aggccccttg ctcgagtggt tcgatgaggc cgaggttgtt gcgctggtgg
      121 ttcgcggttt tcaccggccc aggaaattcg caatttcggc ggacgcaact gggcggctca
      181 gaagaaaacc ctgtatcccg tcacatttga gttgccgcag gagattcaac tgctcctcgg
      241 tctccacccc ttcggcaacc actttgaggc cgagggaatg ggcgagcgaa atgatcgtcg
      301 atacgatggt catgctttcc gaactttggg ccatgcccac gacgaacgac cggtcgacct
      361 tgagcgtgtc cattggcagc ctggcgaggt aattcaacga tgaataccca gtcccgaaat
      421 cgtcgattgc gatggtgacg cccagatcgc gcaacatctt gagccgttcg atattgcgct
      481 ctatgtcttc catcaacagg ctttcggtga tctccagctc gagcgagggc ctcaggcctt
      541 cttgggacgc gagcgcaagc tgcacgtcct gcacgaaatg cgggtcgcgc agttgctgcg
      601 ccgagacgtt gaccgcgacc cgcggtaccg gaagcccctt ggcccgccat cgttgctgat
      661 ccgcgagcgc ttggcgcagc gcccaacgcc ccacttcgaa gatcagcccc gtctcttcca
      721 ggatctggat gaacttcagc ggaggcacca ttccggtttc cgggtcgttc cagcggatca
      781 gcgcctcgac gccggagatg gtgccggtgg ccacatcgaa cttgggctgg taatgcagca
      841 cgaactgctc gagctcaatg gctttgcgca gcctggtttc cagcaccagg atttcggcga
      901 cccgcgcggt catttcgggt gcgtagaata caaagcgctc tttcgcttgc ttggctcttc
      961 tcaaggcggc ttcggcattg tgcaggagag attcagcgct gaatccgtcc gtcgggtaag
     1021 cggaaacccc ggcgcgggca tcgattcgaa gctcctgtcc gttgatcacg aacggcatgc
     1081 cgaagcacgc cttcattccc gcctcgacga gctgggcgag ctcattggcg tcgcgcgcgc
     1141 cgcgtatgat gactccgaag gagtccccga ccacgcgggc cggattggaa aagcgggtta
     1201 tcgtcgcatt ggactccaag cggcgcgcga tcacgcggag caactcgtcg cccgcctggc
     1261 ggcttagcgt gtcgttgacc atgcgcaggc gctccacgtc catcagcgcc acccccacca
     1321 gttccggact gtcgttccgg gtgcgtagct gctgacccaa gcgttcgcta aatagcgcgc
     1381 ggttgggaag cccggtcagg gggtcgtaat aggccaaata atcgaccttc tcttccttgg
     1441 cgatgaactc tagggcgaac gagatgtccc cggccagctc ggtgagcagc ttgatctcgt
     1501 cgtcgtcgaa gaaattccgt tccggcgcat acagggtcag aattccgaac gtcttacctt
     1561 ccacgtacag cggcagcaca atgaccgact tggcccccat ctggatggct gcgcgcctga
     1621 tgctcaaggt atccggcgca agcgcgccgt tcgctgtacc cggctcacgc tcgatttcgt
     1681 tgtcgaaggc gggccgttgt tcgcgcaacg cgacttccgc ggtgcccgcc gccacgaacc
     1741 cggccgagcg atccaaagtt gcgccaccgc cggcggaatc cagtggcagg cccgaattcg
     1801 cgaccggtac gagtttcccg ctcgcagggt ccagcacact gatccagccg atcgtgaatc
     1861 cgccccggtc tacggcgatg cggcaggctt cctgaaacaa ctcctcgcgg tcgcggatgc
     1921 gaacgataag cgcattgatc ccgcttagca ccgcgaacac gcggttgagg cgcttgattc
     1981 tttcttccgt acgcgtttgc tcggtcacat cctggattgt gcaaaaccac atcaaaccat
     2041 cctggtttcc agatcggccc tcaagcggct caatgacctg ccgcacgtga atcaattctc
     2101 catcgccgcg ccacagcctg taatcaacgt ctacgcgcgc gcccgtcttt gccgcctcga
     2161 tgcttttact acgaaacttc tcacggtcat ccacgtgcag gatgtccaac cacgcgcgtg
     2221 tgccctgggg cattgcggcg ggagccacgc caatgagctg tgggagtgtt tcggaccacc
     2281 ttacgaatga cccgcccggg cctgtaatca catgcgcgag tttcgccagc gcctgcgcac
     2341 gctgaaggcc tgcggcctgc tcttgcagcg cgaccttgag ctgcatcctc tcggtaatgt
     2401 cctcggcgat gcctgccgcc cggtagatct tgccctgagc gtcgcggatg gggaatccgc
     2461 ggtcgtggat ccagcgtagc gacccatcgg gacgcacgat ccggtattcc acgtcgagcg
     2521 cctttgcacc cgcggtcatt tcctcgaggc tctttcggac ctgggtgcgg tcctctggat
     2581 ggatggactc caaccagtcc cggtggacct tccctacgtc tccgcgcgag cttccccata
     2641 cgcgctcata ggcaggactg atgtagaggc tttggtctgc gtcgaccgca tgcacccaga
     2701 acacttcgtg catgttttcc gccagttgcc ggaagcgttc ctcgctctcg tggacctgct
     2761 gttcgagtac caggcgcgcg cgtcgctctt cggcttcttt tatcgccctt tccaccgaag
     2821 aaggaagacg cagcaagttg ctcttcagga cgaagtcggt ggcgccgctt ttcagcgcgc
     2881 gaattgcgta ctcttcaccg agggtgcccg agacaaaaat gaatggaatg tcagggtgag
     2941 actgtcttgc aatcctgagt gcttccatgc cgtcaaattt cggcatggaa aagtcggaca
     3001 agatcacgtg cggccggaat tcctcgagct cccgccgata ttcgacggca gcttccaccc
     3061 ggtgtgcgtc acagcggacg ccggcgcgct tgagttctct caccatcagc tcggcgtcgg
     3121 atgcggcgtc ctcgaccatc aggatccgga actgctggct cataagttct taattcattc
     3181 cttccgcctg cccgggtgag tttttgttgt cgtgggtcag aatttcgacc cactgtcggg
     3241 gattctatgc gctccgcgtg atgctcgcta ttggggtttg tcctacaagg atcggcgtgc
     3301 aaggaaaggt ggattaaact cccaagcgca ctatttgaaa gcgcggcgta tgtgcggcgt
     3361 tgagcgtgcg tacttgagct gcagccgatt tttcggtggc gcgtttgggg ggcgatttca
     3421 actcgagtga cgtagcgacc gcccgtatcg ttagttgcgc gtggcctgag acgactgcaa
     3481 cgcatttgcg agcggcgccg gtcgacccag gaaataccct tgtgctttgt tgacgcctaa
     3541 cgaacgcagt accctgagct ccgatgcggt ttcaactcct tcggcgacga tcttgctgcc
     3601 agtctcgtgg gcaaatcgga tgagcgccga ggccagggcg cgacgggcgg catccgtgtc
     3661 gatgtcccgg gtaaggctca tgtcgagttt gatgatgtcg ggctgaagcc gaagtatgtg
     3721 gcgaaagctg gcaaagcctg ctctggcgtc gtccactgcg aggcgcaggc cctgagttcg
     3781 cagcggagcc agggcgttgg tcagttccga gtactcgtcg atcgtggcat gttcagtgat
     3841 ttccagcacg agcctttcca agagcatgcc ttcgagaacg cgccggaggt ctccgttgat
     3901 gagcgcctcg ggcgagacat tgaaagacag gtaaacgtct tgagggaggc tatcgagggc
     3961 tgccattgcc aactcgactg ccctcatttc aagttgcgcg cccatgccga cgctcgccgc
     4021 ctcattgaac cagacgtccg gaccgcggag cggcgtggca gagaaccgag tgagggactc
     4081 gaagcccacc accttatcct gcgcgacatt aattattggc tggtaaacga tcgaaagacc
     4141 gtcgccgctt atgacggact ggattcgttt cgccttctcg tcgcgctgtt tgcccttctc
     4201 aagatttcgt tcgatgacat cggcggcaag gtcggcgaaa acgcgcatca ttccgaggtc
     4261 gcgctgatta agggagaggt cgggggtgaa gctgaagcag cagaaggtcc catagacgct
     4321 tccatccttg agtttaatcg gaacgcttag gtgagcccca accggtaatg ccctcgttac
     4381 cggcagttcc atggcggcgg gaacggccgt ggcatcctgg atgagttctg gcagtcggcc
     4441 gtcgacaact cgttggcagt agctcgcctc cagtggatct gctccgccca cctttaccgg
     4501 ctggttctcc aaagatgagt cgacatgacg gaatacccgt tggccatccg tgaactcgga
     4561 caggaatgcc acgtccatcc caaggtgtga gcgcaccgca cgcaaaaaat cgggcaggag
     4621 ttgatcaagc gaaccgccta gtgcccgtgg gttgccactt aataattttg atagcgagct
     4681 ttcgtccctt ggtgtggcca ttggcgtatc tcaacatcat ttgcagagtc ctggcagtgc
     4741 agcggctgca gggtcgtgtt caatcccatg gtgttcacgc cgaaagcagc cgggtaacgg
     4801 aatcttgctt ggggtaagtc cgggggtaac tggaccacgg gcatacctca acgtctagga
     4861 ttggtgcggc ttgccgagtg ctatttgaat gcagccgtgc caccacttct tccgcatcgg
     4921 cagacatcgg tcagaagttg tcttcctctt cctcgtcgtc gagttcctct tcctcgtcgt
     4981 aggggtcctc ctcctcatcc cgccagtcgc cggagtcgat cacttcggtg acgatgtctt
     5041 cgatgcggtc acgcacatcc tgctcggcct ggcctctgac gccatccact tcggtatagg
     5101 gttcttcgtc gtcctctatc gagacgatcg cgtcgggcca ctcgtcctgg aatgcctcca
     5161 gcagttggtc cttgaagcgc tggtactcct cgtcagagac gatgccgagc ggctcgtgag
     5221 aagtgtagtg ggtgatcgaa agggtcgagg ccatgtttgc cggttgccgc gctgatcgtt
     5281 ttcaaaagga taccgaaatc tcgaacgcat tctatcctcg gctgcaaaat tcgctttacc
     5341 cgcttcggca gagcaattgt cgtgccgttg tccgtagcca ggcggcgatg ccgtcaatca
     5401 agtgccggaa aggctaaccg acacgctcca gcacgtcgcg atcgatctcg gctagtgtgg
     5461 ggcggcccag cagggtcagc gtgatgtcga tctcgtcgcg cagtatttcc agcgcgcgct
     5521 ctactcccgc ctggccggcg gaggccagcc cgtaaagcca ggcgcgaccg atcatgcagg
     5581 cttttgcgcc gagtgcgcgc gcacgtacca cgtcggcgcc acggcgaatg ccgccgtcga
     5641 gcacgacttc aatccggtct cccaccgcgt cggcgattgc cggcaatgcg tgcacagcgg
     5701 atagcgcgcc ctcgagttgc cgcccgccgt gattggaaac gatcaccccg tccgcgccat
     5761 gcgctacggc aagcttggca tcctcgacgc tgaggatgcc cttgagcagg accggcccgc
     5821 cccacgccga cttgaaccac tcgacgtcgc gccagttcac cgccaggtcg tactggccgg
     5881 cgatgaattc ggtgatggtg actgcgtcgg tggcggcagc cttggtgccc tcgaaattcc
     5941 tgaaggcgat gcgcgggccc aggcccacgt cgaagagcca ggagaaatgg cgggcgaagt
     6001 cgaggatcgt cgcgatggtg aagcgcggcg gcacggtgaa gccgttgcgc atgtcgcgct
     6061 cgcgcggacc ctgcaccttg gtgtccacgg tcaggaccag cgccgtgcag cgcgacgccc
     6121 gcgcgcgctc gatgaactcc ttggtgagtc cccggtcgcg cagcacgtag agctggaacc
     6181 actttggctg agtggtctcg gtggtgatct cctcgatgct gcaggtggcc attgtggaca
     6241 ggcagtaggg cacgccgtat ttctctgcgg cgcgggcagc ggccagctcg ccgcggcgcg
     6301 acagcagtcc ggcgagtccg gtcggcgcga gaaccaatgg cgaggcgcag cgctggccga
     6361 acagggtgat cgactggtcg cgcctggaaa cgtcggtcag cacccgggta cggaaacgcc
     6421 acttctggaa atcctcgcgg ttggcgcgca gcgtggtttc gtcctgggct ccgccatcga
     6481 tgaactcgaa gataaccttc ggcagacgcc ggcgtgcccg gctacgcaga tcttcgatgt
     6541 tgattgcttg catggactgt tcttgttgaa ggtggaaaaa agttgaggtc tgatcggatt
     6601 ttagttccga cataacgtgc tgcgcacatt agtctccact gaaaccccgt tcacttctta
     6661 tgggcgaacg gggttttctc cccaatacat cggggcgtcg tactttctcg gtttcagcaa
     6721 atcctccagg taaagcgatg gcgccatctc ccaggcgtac tcgttgaagc gcgccaacat
     6781 ctcctgtacc cgttgcggtt cgcgctcggc ccggttgtct tcctccgacg ggtcgtcctg
     6841 gatattgaac agttcgtagc gcaccggcaa gcgcgagtag acgatgagct tccagttctc
     6901 aatcagcagg gcgccgcggc tgtcttccat tgccagcagc atttctttgc gcgcacttgg
     6961 cttaccgtcg gcaatcgtgg cccactggtc gatgccgtcg actcgccgtt tctgttcgag
     7021 cttcgcaccc gcgaccctca gcaaagtcgg gtacatgtcg gtgacgtgga tgcgttcggc
     7081 aaccacgccc ggctggattc tacccggcca cgcagcgaac gccgccaccc gcatcgaacc
     7141 ttcgtgcagt ccacccttgc cgtcctggaa ggggccgttg tcggcagcgg gtttactgac
     7201 atcgccgtcg ccggtcgcat acttgttcgg cagtgccccg ccgttgtcgc tgtggaacac
     7261 tatcagcgtg ttcgccgcca tgcctttcga ttccagcgcc gaggtcaatg cccctatggc
     7321 gtcgtcgagt gcggtgacca tcgccacata ggcgcggcgg gtttcgtcgg caatgtcccg
     7381 gtacatttcc aggtattccc tcggtgcctg caacggcatc tgcggcgcgt tgaaggacag
     7441 gtagaggaac aacggtgtgg tcgcgtcgtg ccctgaaatc aggctggctg cctccctgcc
     7501 caacagggtc gtgacgtagc ctgcctcctt cacctgccgt tcattgcggt gccagtcggc
     7561 ctcgccgatc ctgtcggtct tcctgaaata gtcgatctcg ccgttgaagc tgccgtagaa
     7621 actgtcgaaa ccgcgccggg tcggccagaa ttccttcttg aaatggccga gttgccactt
     7681 tccgagcatc gcggtgcgat agccggcctc tttcagcgcc tgcggcaaca tccgttcatc
     7741 ggccggcaat ccgtaccggc tccacggcag aatggaaagc gtctgcaggc cgtagcgcat
     7801 cggatagcgg ccggtcatca atgccgcgcg agtctgggtc gagtagggga gcgtataaaa
     7861 cttctcgagg cgtgcgccgc cttgtgccag cttgtcgagg tggggcgtcc tgatagggct
     7921 gccgtggtaa cccacgtcct tccagccgag gtcgtcggcg aggatgtaga cgatgtgcgg
     7981 ttgctgcgcg gcaccgatgc atgcataggc gcaaccgaat aacgctgccg cgagtcgcag
     8041 agccaggttc acttgctcct ccatcgtttt gcaccggatg ttacctgaga tcgcgcgctg
     8101 ccagcctgcg agccggatgc ccacggcttc cggcgcatgc ttgcctcgca tggggattcc
     8161 cggctgcgtt acaatcgccc cggtcatgaa ggatagtgaa gtcatactca gtgttctggg
     8221 cagcatcgag cgccgcctgt ggttgcgccg ggcgcttcag gagattacct acggcgcctg
     8281 cgtcgtcctg ttttccctga ttgccttcca gtttgtaggc gtggcgttga gttcggcaat
     8341 tccggcggtc ggaaccggcg cccggacggc ggtcgtagcg gcggtggtga tgctcggggc
     8401 ttatattgcg tggcgcgcat tgcgtcaggt gacgctcgcg caagctgcgg gcgaagccga
     8461 tgcacgcgcc cgcctgaagg acgaactgaa atccgcttac tggttcgttt ccatgcccga
     8521 gccatccccg ttcgcggaac tgcaaattgc ccgcgcggct gcgaccgcac gccggctcga
     8581 tcccggcgcg atcgtccccg ggcggccgcc ccgcagcctg tacgccgttg ccggtctggg
     8641 cgcggccctg ctgctggtta cctggattgc cccacagcaa tcgcattcat gggacggtgc
     8701 agatgcagca acggccgcgg ccggggaaac gcaggacctg cgcgcactcc tcgaaggcgc
     8761 gccgcaggat cccgagattg agaagctcga tcgcgcgctc gaggaattgc agcgcgctga
     8821 cgcgaccgcc gaagagaagg cgcgagcagc cgcgcaggcg cgcgatgcga tcgagcaggc
     8881 caacatggaa gctgcggctg cgcgcgaggg gctggtcagg ttggccgggg ccatgagggc
     8941 gaacccgcgc ttcgagaagg ccgcggaagc gcttcgggcc gggcgcaacg acgaggcgct
     9001 ggcgttgctt cgtcaattgc gcgccgaggt cggcggccgg gacacacgtg cgctggagaa
     9061 ggacgggatc cggccgctgt cggacagtga gaaaggcctg gaccgcggca tcgaggaaag
     9121 actcgcggaa ggcagccgcg acctgagcag catcaacgcc agggtggacc aggatgcact
     9181 cgaccgcgtg gtgaacgccc tccaggaagc caaccaacgg ctcgaggtac agagtcgcgt
     9241 aatcgaagtc aaacggcgga tgcaggaggc aatgatcccg acgtcgcagc ggagcgcgct
     9301 gaccgcgagc cagttcgatt ccagcgacaa cgcgcccaat gcgaccgctg ctccggatac
     9361 cggcaacgca gacatccgcg gcggcgccat gtttcgccag ggcgcggtga cgcgcagaaa
     9421 tgacgatgaa cccgcccgcg acggcaatat gagtggcagt gccgccggca attccgaggc
     9481 tctggcgctg gagggtgcag cgacccggcg tctggatgcg caactcaagc tcgaagccat
     9541 ccatcgccgc gacgatgccg acgacgacga aaaggccgac cagggctggt tttattccgc
     9601 cagccagcag caaaaatcgc tgctcggctt cgaggacgtg cgcgcgcgct cgagcttcga
     9661 ccgcgagaac gcgatgagcg tcgagcgcgt gccgatccgc cagaaacatg ccgtaaggga
//
LOCUS       DASQOP010000331         2219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_975661, whole genome shotgun sequence.
ACCESSION   DASQOP010000331 DASQOP010000000
VERSION     DASQOP010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975661"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="VE030_12920"
     CDS             complement(<1..557)
                     /locus_tag="VE030_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24355.1"
                     /translation="MAENSKAITADSQVARIFEWRRGFNTIHLIDVGVHLGLFQALVE
                     KPGLTGRELAERLGLHAPYVEVWCKTAYGLEILDADNAGSFKLAPYFDLILASPTHPR
                     YLGGYVRLGTEVAAEDFRRCREAFRTGTVKPFQGRGDHFNQAVAESTQGLQLVTAKKI
                     LPGLAGLAGKLAAGGAILEIGCGTG"
     gene            665..1153
                     /locus_tag="VE030_12925"
     CDS             665..1153
                     /locus_tag="VE030_12925"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZF24356.1"
                     /translation="MNLARSLLVLLLLAASASWAVPEVDQPAPALKGMLFSGKPFDLK
                     EMRGKVVLVNFYSSYCSFCAYEIGNLETFYEQHHDEGFEVIVVGIDSLEDRHRVERML
                     GIYGLPGVMADDLEQSGFERKYPTPTAFVIDREGILRSRTRGSKTRPYFAEHVLPLLR
                     TQ"
     gene            complement(1179..>2219)
                     /locus_tag="VE030_12930"
     CDS             complement(1179..>2219)
                     /locus_tag="VE030_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HZF24357.1"
                     /translation="LAMIRSWNTATLIVKNGELTPQDVARLQEFCRSRGFDLVWHPGM
                     RARDADTFNRLQRPYFYEGAVALLGGQRADFIERYKFNIAPATDDRPYFFHFFRWKTL
                     PELLQLRDRGGLPLLDWGYPLLIATLVQALVLGAALILLPLARLRSPGARIGAGVRWR
                     TAAYFSALGTGFMFVEIAFIQKFVLFLGHPLYAVAVVLFAFMLCAGLGSLLSQHLAKE
                     IAPGFALLVPPIAAIALLCGVYLIWLPGAINALIALPDAARIAITVLLILPLGLSMGM
                     PFPLGLEALGARTPALVPWAWGINACTAVAGAVLASVLAIHAGFNAVLIVAIVLYAST
                     LFSFPETAVKRQ"
BASE COUNT          443 a          717 c          679 g          380 t
ORIGIN      
        1 ttgccggtgc cgcagccgat ttccaggatg gcgccgccgg ccgccagctt gccggcaagt
       61 ccggcgaggc ccggaaggat tttcttcgcg gttaccaact gcagcccctg ggtggactcg
      121 gcgaccgcct ggttgaagtg atcgcccctg ccctggaacg gcttgacggt gccggtccgg
      181 aacgcttcgc ggcagcgacg gaaatcctcc gccgcgactt cggtgccgag ccggacgtag
      241 ccgcccagat agcggggatg agtcggactg gcaagaatca gatcgaaata aggcgcgagt
      301 ttgaaactgc cggcgttatc ggcgtccaga atctccaggc cgtacgctgt cttgcaccag
      361 acctcgacgt acggtgcatg caggcccaga cgctcggcca gttccctgcc ggtcagcccc
      421 ggcttttcga ccagcgcctg gaacagtccg aggtgcacgc cgacatcgat gaggtgaatg
      481 gtgttgaatc cgcgccgcca ttcgaaaatc ctggcgacct gcgagtccgc cgtgatcgcc
      541 ttgctgtttt ccgccattgc cggtatcccc atgttctgca agtctgcgcg gcaaagtata
      601 ctggcctgat ctatatctga ccgcatccgt cttccgcgcc gttatgccat gcggagcctg
      661 tccgatgaac cttgcaagaa gtctcctcgt tctgctgctg ctagccgcgt cggcgtcatg
      721 ggcggtgccc gaagttgacc agcccgcgcc ggcactcaag ggcatgctgt tctcgggcaa
      781 gccattcgat ctgaaggaaa tgcgcggcaa ggtggtgctg gtcaatttct actccagcta
      841 ctgcagtttc tgcgcctacg agatcggcaa tctcgaaacc ttctacgagc agcaccacga
      901 cgagggcttc gaggtcatcg tcgtcggcat cgacagcctg gaagatcgcc atcgcgtcga
      961 acgcatgctc gggatatatg gactgccggg tgtgatggcc gacgatctcg agcagagcgg
     1021 attcgaacgc aaatatccga cacccaccgc tttcgtgatc gaccgtgagg gcatcctgcg
     1081 aagccggaca cggggttcaa agacacgccc ctacttcgcc gagcacgtgc tgccgctgct
     1141 gcgcacgcag tgacgccgtg tgcccgcaag tggggccgtc attgccgctt caccgcagtc
     1201 tcaggaaagc taaacagcgt cgacgcgtac agtacgatcg cgacgatcag taccgcgttg
     1261 aagccggcgt gaatcgccag caccgatgcc agcaccgcac cggctaccgc ggtgcaggca
     1321 ttgattcccc acgcccaggg caccaatgcg ggtgttcgcg cgcccagcgc ctccagtccc
     1381 agcggaaacg gcatgcccat gctcaggccg agcggcagga tcagcagcac ggtgattgcg
     1441 atcctggccg cgtccggcag cgctatcagc gcgttgatgg ccccgggcag ccagatcaaa
     1501 tagaccccgc acaacagcgc gatcgccgca atcggcggca cgagcaacgc gaaacccggc
     1561 gcgatctcct ttgccagatg ctgcgacagc aggctgccca ggcccgcgca cagcatgaac
     1621 gcgaacaaca ccaccgccac ggcatacagc ggatggccga gaaaaagaac gaatttctgg
     1681 atgaacgcaa tctcgacgaa catgaatccg gttccgagag cggagaagta ggcagcagtg
     1741 cgccaccgca ctcccgcgcc gatgcgcgca cctgggctgc gcagtcgggc cagcggcaga
     1801 aggatcagtg ccgcgcccag cacgagcgcc tgcaccagcg tggcgatcag caacggatat
     1861 ccccaatcca gcaaaggcaa gccgccgcgg tcccgcaatt gcagcagttc gggcagcgtt
     1921 ttccagcgga aaaaatggaa gaaatagggg cggtcgtcgg tcgcgggcgc gatgttgaac
     1981 ttgtagcgct cgataaagtc cgcgcgttga ccgccgagca gcgccaccgc gccttcatag
     2041 aagtaaggcc gttgcagccg gttgaaggta tcggcgtccc gggcgcgcat tcccggatgc
     2101 cacacgagat cgaagccgcg gcttctgcag aactcctgca gtcgcgcgac gtcctgcggc
     2161 gtcaattcgc cgtttttcac gatcagagtg gcggtattcc agctgcggat catcgccag
//
LOCUS       DASQOP010000332         6939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1583158, whole genome shotgun sequence.
ACCESSION   DASQOP010000332 DASQOP010000000
VERSION     DASQOP010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6939
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1583158"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..142
                     /locus_tag="VE030_12935"
     CDS             <1..142
                     /locus_tag="VE030_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HZF24358.1"
                     /translation="EMVMPGDNVAITVQLIQPIAMEEGLRFAIREGGRTVGAGVVSKV
                     IE"
     gene            148..456
                     /gene="rpsJ"
                     /locus_tag="VE030_12940"
     CDS             148..456
                     /gene="rpsJ"
                     /locus_tag="VE030_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HZF24359.1"
                     /translation="MQNQKIRIRLKAFDYRLIDQSAIEIVETAKRTGAVVKGPVPLPT
                     RIERFDVLRSPHVNKTSRDQFEIRTHLRLMDIIDPTDKTVDALMKLDLPAGVDVEIKL
                     "
     gene            542..1189
                     /gene="rplC"
                     /locus_tag="VE030_12945"
     CDS             542..1189
                     /gene="rplC"
                     /locus_tag="VE030_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HZF24360.1"
                     /translation="MSLGLVGRKIGMTRLFTDDGDTVPVTVVDVSNNRVTQIKTPETD
                     GYAAVQVTFGKRRASRVSKPAAGHFAKAAVEAGHILREFRVEADQLASLKVGGQVGVD
                     IFKVGQFVDVTGTSIGKGFAGVIKRHHFSSNRASHGNSVSHNKPGSISMAQDPGRVFP
                     GKRMSGHLGAVKRTAQKLEIVRIDAERQLLLIKGAVPGSEGGDVIVHPSVKTRAA"
     gene            1189..1809
                     /gene="rplD"
                     /locus_tag="VE030_12950"
     CDS             1189..1809
                     /gene="rplD"
                     /locus_tag="VE030_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HZF24361.1"
                     /translation="MELKLINDKGEATASVKASDELFGRAYNESLVHQVVTAYLANAR
                     LGTRAQKGRSDIAKSTRKPWRQKGTGRARAGMASSPLWRGGGKIFPNSPDENFSHKVN
                     RRMFRAGLASILSQLAREGRLAVVENFKVDSPKTKQLAQKVKGMGMDNVLIITDSLDE
                     NLVLSARNLVNVLVVEPGQADPVSLLRFSNTLITKGAVAKFEEMLA"
     gene            1836..2168
                     /gene="rplW"
                     /locus_tag="VE030_12955"
     CDS             1836..2168
                     /gene="rplW"
                     /locus_tag="VE030_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HZF24362.1"
                     /translation="MKQDGVRQNAVNQERLMQVLLAPVVSEKSTYVADKNEQVIFRVA
                     PDATKPEIKAAVELLFKVQVKSVQVANVKGKEKRFGRFIGRRRHWKKAYVCLKPGQEI
                     NFAAGEAK"
     gene            2169..2996
                     /gene="rplB"
                     /locus_tag="VE030_12960"
     CDS             2169..2996
                     /gene="rplB"
                     /locus_tag="VE030_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HZF24363.1"
                     /translation="MALVKVKPTSPGRRALVKVVNANLHKGKPVAALVEKQSKRAGRN
                     NRGHITTRHQGGGHKHHYRIVDFVRDKDGIAARIERLEYDPNRSANLALLCYADGERR
                     YIIAPKGVTVGLEVINGAQAPIKVGNTLPLRNVPVGTTVHCVEMLPGKGAQLARSAGT
                     SVQLLAREGTYAQLRLRSGEIRKVHVDCRATIGEVGNEEHNLESIGKAGRVRWRGVRP
                     TVRGVAMNPIDHPHGGGEGKTAAGRDPVSPWGTPTKGYKTRRNKRTRGMIVRDRHGK"
     gene            3010..3273
                     /gene="rpsS"
                     /locus_tag="VE030_12965"
                     /pseudo
     CDS             3010..3273
                     /gene="rpsS"
                     /locus_tag="VE030_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006682632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
     gene            3378..3713
                     /gene="rplV"
                     /locus_tag="VE030_12970"
     CDS             3378..3713
                     /gene="rplV"
                     /locus_tag="VE030_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HZF24364.1"
                     /translation="MRTSAILRGVRLSAQKGRLVADQVRGLPVDKALSLLAFSPKKGA
                     KIIRKVLESAIANAEHNQGADIDELTVTTIHVEQGTSLKRFMPRAKGRGFKITKPTCH
                     IYVTVGDKE"
     gene            3716..4399
                     /gene="rpsC"
                     /locus_tag="VE030_12975"
                     /pseudo
     CDS             3716..4399
                     /gene="rpsC"
                     /locus_tag="VE030_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415738.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
     gene            4410..4823
                     /gene="rplP"
                     /locus_tag="VE030_12980"
     CDS             4410..4823
                     /gene="rplP"
                     /locus_tag="VE030_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011901907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HZF24365.1"
                     /translation="MLQPARRKYRKEQKGRNTGVATRGAKVSFGEYGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRIFPDKPISRKPAEVRMGNGKGNPEYYVAEIQPGKV
                     LYEMDGVDETLAREAFRLAASKLPIQTTFVIRQVG"
     gene            4827..5021
                     /gene="rpmC"
                     /locus_tag="VE030_12985"
     CDS             4827..5021
                     /gene="rpmC"
                     /locus_tag="VE030_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014112153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HZF24366.1"
                     /translation="MKASELRAKTAADLNKELNELLKAQFSLRMQLATQQLNNTSEIK
                     KVRRDIARVRTLIGEKAKQS"
     gene            5018..5290
                     /gene="rpsQ"
                     /locus_tag="VE030_12990"
     CDS             5018..5290
                     /gene="rpsQ"
                     /locus_tag="VE030_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020290702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HZF24367.1"
                     /translation="MNETQQSNARKLTGRVVSDKMNKTVTVLVERKVKHPLYGKFVRR
                     SKKYHAHDENNEFHPGDLVLIEETRPMSRTKTWKVVKLVEKAKEEV"
     gene            5447..5815
                     /gene="rplN"
                     /locus_tag="VE030_12995"
     CDS             5447..5815
                     /gene="rplN"
                     /locus_tag="VE030_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HZF24368.1"
                     /translation="MIQMQSRLDVADNTGARSVMCIKVLGGSKRRYAGVGDVIKVSIK
                     DCAPRGRVKKGEIYHAVVVRTRSGVRRQDGSLVKFDTNAAVLLNAKLEPIGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            5825..6142
                     /gene="rplX"
                     /locus_tag="VE030_13000"
     CDS             5825..6142
                     /gene="rplX"
                     /locus_tag="VE030_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HZF24369.1"
                     /translation="MRKIRKGDNVVVITGKDKGKRGTVLRVLGDDYILVEGANRVKKH
                     QKPNPMKGTTGGIIDKEMPIHVSNVAVFNPTTQKADRIGVKQMDDGRRVRIYKSNGEM
                     LDA"
     gene            6314..6844
                     /gene="rplE"
                     /locus_tag="VE030_13005"
     CDS             6314..6844
                     /gene="rplE"
                     /locus_tag="VE030_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015420243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HZF24370.1"
                     /translation="MRQHYREAVVPALMKQFGYKTAMQVPRIDKIVLNMGVGEAVNDK
                     KILDNAVGDMTRIAGQKPVVTKAKKSIAGFKIRKDYPIGCMVTLRRGRMYEFLDRLVS
                     IAIPRIRDFRGISARSFDGRGNFNLGVKEQIIFPEIEYDKIDVLRGLNITITTTAKTD
                     DEARALLAAFKFPFKN"
BASE COUNT         1566 a         2076 c         2217 g         1080 t
ORIGIN      
        1 cgagatggtg atgcctgggg acaacgtggc cattaccgtg cagctcattc agcccattgc
       61 gatggaagag ggcttgcgct ttgccattcg cgagggcggc cgcaccgtgg gtgcgggcgt
      121 ggtgtcaaag gtcatcgagt agagaaaatg caaaaccaga aaatccgcat tcgccttaag
      181 gccttcgact atcgcctgat cgatcagtcg gcgatcgaaa tcgtcgagac cgccaagcgc
      241 accggcgcag tggtcaaagg cccggttccg ctgcccaccc gcatcgagcg cttcgatgtg
      301 ctgcgcagcc cgcacgtgaa caagacttcg cgcgatcagt tcgagatccg cacccatctg
      361 cggctgatgg acatcatcga tcccaccgac aagaccgtgg atgcgctgat gaagctcgat
      421 ctgcctgcag gcgtggatgt agaaatcaag ctgtaggaag cagtaccccg ggatttcccc
      481 ggataaaaat agattggctg gtcaattgaa accggcccct tttgtcaaag ggaaacaaat
      541 aatgagttta ggactggtcg gtcgcaagat cggcatgacg cgactgttta ccgacgacgg
      601 cgataccgtc ccggtgacgg tcgtggacgt gtcgaacaat cgcgtgaccc agatcaagac
      661 gcccgagacc gacggctatg ccgcggtgca ggtgacgttc ggcaagcgcc gcgccagcag
      721 ggtttccaag cccgcggccg gccactttgc caaggccgcc gtcgaagcgg gtcacatcct
      781 cagggaattc cgtgtcgaag ccgaccagct tgccagcctg aaggtcggtg gccaggttgg
      841 cgtcgacatc ttcaaggtcg ggcagtttgt ggacgtgacc ggcacctcga tcggcaaggg
      901 ctttgccggc gtgatcaagc gccatcactt ctcctccaac cgcgccagcc acggcaactc
      961 ggtgtcgcac aacaagccgg gttcgatttc gatggcgcag gatccgggcc gcgtatttcc
     1021 cggcaagagg atgtccggcc atctgggcgc cgtgaagcgc accgcgcaga aactggaaat
     1081 cgtgcgcatc gacgccgagc gccagttgct cctgatcaag ggtgcagttc ccggttccga
     1141 aggcggcgac gtgatcgtgc atccttcggt gaagacgagg gcggcgtaat ggaactaaaa
     1201 ctgatcaacg acaagggtga ggccaccgcc agtgtcaagg cctccgacga gctgttcgga
     1261 cgcgcgtaca acgagtcgct ggtgcaccag gtggtcactg cctatttggc caacgcccgt
     1321 ctcggcaccc gtgcccagaa ggggcgcagc gacatcgcca agtccacgcg caagccctgg
     1381 cgccagaagg gcaccggccg cgcgcgcgcc ggcatggcgt ccagcccgct gtggcgtggc
     1441 ggcggcaaga ttttccccaa cagcccggat gagaacttca gccacaaggt caaccgcagg
     1501 atgttccgtg ccggcctggc ctcgatcctg tcgcagctgg cccgcgaagg ccgtctcgcc
     1561 gtggtggaga atttcaaggt tgattcgccc aagaccaagc agctggcgca gaaggtcaag
     1621 ggcatgggca tggacaacgt gctgatcatt accgacagcc tggacgagaa tctggtgctg
     1681 tcggcgcgca atctcgtcaa tgtgctggtg gtcgaacccg ggcaggcgga tccggtcagc
     1741 ctgctgcgct tcagcaatac gctgatcacc aagggtgcgg tggcgaagtt cgaggagatg
     1801 ctcgcgtgaa cgcagctgcg attaaaaaga acgctatcaa acaggacggt gtcaggcaaa
     1861 acgccgtcaa tcaggaacgg ctgatgcagg tgctgctcgc gccggtggta tcggaaaaga
     1921 gcacctacgt cgcggacaag aatgagcagg tgatcttccg tgtcgcgccg gatgccacca
     1981 agcccgagat caaggctgcg gtggagttgc tgttcaaggt gcaggtgaaa agcgtccagg
     2041 tcgccaacgt gaagggcaag gaaaagcgtt tcggccgctt catcggtcgc cgccggcact
     2101 ggaagaaggc ttatgtctgc ctgaagccgg gccaggagat caacttcgca gcaggggagg
     2161 ccaagtaaat ggcgctcgtc aaagtcaaac cgacatcccc cggccggcgc gcgctggtca
     2221 aggtcgtcaa cgccaacctg cacaagggca agccggttgc cgctctggtc gagaaacaaa
     2281 gcaagcgagc cggccgcaac aaccgcggcc acatcaccac gcgccatcag ggcggggggc
     2341 acaagcatca ctatcgcatc gtcgatttcg tgcgcgacaa ggacggcatt gccgccagga
     2401 tcgagcgcct cgaatacgac ccgaaccgca gcgccaatct tgcgctgctg tgttacgccg
     2461 acggcgagcg ccgttacatc atcgcgccca agggcgtgac ggtgggactg gaggtcatca
     2521 acggggcgca ggcgccgatc aaggtcggca atacgctgcc gctgcgcaat gtgccggtgg
     2581 gcaccacggt ccactgcgtc gaaatgctgc ccggcaaagg cgcgcaactg gcgcgttccg
     2641 cggggacctc ggtgcagttg ctggcgcgcg aaggcaccta tgcgcagttg cgcctgcgtt
     2701 ccggcgagat ccgcaaggtg cacgtcgact gccgcgccac catcggggaa gtcggcaacg
     2761 aagagcacaa cctcgagtcc atcggcaagg ccggacgcgt gcgctggcgc ggagtacgcc
     2821 cgacagtgcg cggcgtggcg atgaatccga tcgaccatcc gcacggcggc ggcgaaggca
     2881 agaccgctgc cggccgcgac ccggtgagcc cgtggggaac gccgaccaag ggttacaaga
     2941 cccgccgcaa caagcgcacc cgcggcatga tcgtgcgcga tcgccacggc aagtaagggg
     3001 taaggacaca tggcacgttc catcaagaaa ggcccgttcg tcgatcatca cctggtacag
     3061 aaggtggatg cggcgcgcgc caccaacgac aagcgcccga tcaggacctg gtcgcgccgt
     3121 tccaccattc ttccggactt cgtggggctc accatcgcca ttcacaacgg ccgacagcac
     3181 gtgcctgtgt atatcagcga gaacatggtc gggcacaagc tcggcgaatt tgcgctgacc
     3241 cgcacgttca aggtccacac cggggacaaa aaggtcgtgg cgccaacttc cgggggctcg
     3301 ccatcatcgg caccggggtc ggcgtccccg gcagcacctg cagcaggcaa gaagtaaggg
     3361 cgatgggaga gatgacgatg agaacttctg caattttgcg cggcgtgcgc ctgtcggccc
     3421 agaagggacg cctggtcgcc gatcaggtgc gcggcctgcc ggtggacaag gcgctgagcc
     3481 tgctcgcctt cagcccgaag aagggcgcca agatcatccg caaggtgctg gagtccgcga
     3541 tcgccaatgc cgaacacaac cagggcgccg acatcgacga gttgacggtc acgaccatcc
     3601 acgtcgaaca gggtacgagc ctgaagcgtt tcatgccgcg cgccaagggc cgcggcttca
     3661 agatcaccaa gcctacctgc cacatttacg tgacggtcgg ggacaaggag taagcatggg
     3721 acagaaaatt cacccgaccg ggttccggct gtcggtgcag cgcaactgga cgtcgcgctg
     3781 gtactcgaac accaagaatt tcccggcgat gctgcaggag gacatcaagg ttcgcgactt
     3841 cctgaagaag aaactggcgc acgccgcggt cagcaaagtg gtgatcgagc gcccggcgaa
     3901 aaacgccaaa atcaccgtgt acagcgcccg tcccggtgtg gtgatcggca agaagggcga
     3961 ggacatcgaa catttgcgcg ccgaactgca gaagctcatg ggcgtgccgg tgcatgtgaa
     4021 catcgaggaa gtgcgcaaac ccgagatcga cgcgcagctg atcgcggatt ccatcgccca
     4081 gcaactcgag aagcgcatca tgttccgccg ggccatgaag cgcgcgatcc agaatgccat
     4141 gcgcctgggc gcgcagggca tcaagatcat gagcgccgga cgcctgaacg ggatcgaaat
     4201 cgcccgcacc gagtggtacc gcgaaggccg cgtgccgctc cataccctgc gtgccgacat
     4261 cgactacgga ttttccgagg ccaagaccaa ttacggagtg atcggcatca aggtgtgggt
     4321 attcaagggc gaaatgctcg gccgcggcga gcagccggtg gtggcgccag cgccggagcc
     4381 ggagaggaag gcaagacgag gagccaggaa tgctgcaacc agctagaagg aaataccgca
     4441 aggaacagaa aggccgcaac accggcgtcg ccacccgcgg cgccaaggtg agcttcggcg
     4501 aatacggcct gaaggccgtc ggccgcggtc gtctgaccgc gcgccagatc gaagccgcgc
     4561 gccgtgccat gacccgccat atcaagcgcg gcggacggat ctggattcgc atctttccgg
     4621 acaagccgat ctcgcgcaag ccggcggaag ttcgcatggg taacggcaag ggcaatccgg
     4681 agtactacgt tgcggagatc cagcccggca aggtgctcta cgagatggac ggcgtggacg
     4741 agacgctggc gcgcgaggca ttccggctgg ccgcctccaa gctgccgatc cagaccacgt
     4801 tcgtaatcag acaggtggga taagcgatga aagcgagcga actccgggcc aagaccgcgg
     4861 ccgacctcaa caaggagctc aacgagcttc tcaaggcgca gttctcgctg cgcatgcagc
     4921 ttgccactca gcagttgaac aacaccagcg aaatcaagaa ggtgcgccgc gacatcgcgc
     4981 gcgtgcgcac tctgatcggc gagaaggcca aacagtcatg aatgagaccc agcaatccaa
     5041 cgcgcgcaag ctgaccggac gcgtggtcag cgacaagatg aacaagaccg tcacggtcct
     5101 ggtggagcgc aaggtcaagc atccgctcta tggcaagttc gtccgccgct ccaagaaata
     5161 ccacgcgcac gacgagaaca acgaattcca tccgggcgac ctggtgttga tcgaggaaac
     5221 ccggcccatg tccaggacca agacctggaa agtggtgaaa ctggttgaga aggccaagga
     5281 agaagtctga aatccttgct ttggcggagt tttttccata gaatgccgcc tttttcccgg
     5341 gtcgcctttg tccgtggcgc cccgacttcc gacccggacg ggttccaaaa ctggtctgcc
     5401 gaagtagaaa acaccaggcg ggcgaagttg gagagagagt aagcagatga ttcaaatgca
     5461 atccaggctg gatgttgccg acaacaccgg cgcgcgctcc gtcatgtgca tcaaggtgct
     5521 gggcggttcc aagcgccgct atgcgggggt gggcgacgtc atcaaggtga gcatcaagga
     5581 ctgcgcgcca cgcgggcgcg tcaagaaggg cgagatctac cacgcggtgg tggtgcgcac
     5641 ccgcagcggc gtgcgccgcc aggacggttc gctggtgaag ttcgatacca acgccgccgt
     5701 cttgctcaat gccaagctcg agcccatcgg cacccgcata ttcgggccgg tgacgcgcga
     5761 actgcgaacc gagcgattca tgaagatcgt ctcgctcgcg ccggaagtgt tgtaaggaac
     5821 aatcatgcgc aagatccgca agggcgacaa cgtcgtggtc atcaccggca aggacaaggg
     5881 caagcgcggc accgtgctgc gcgtgctcgg cgatgactac atcctggtcg agggcgccaa
     5941 ccgcgtcaag aagcaccaga agcccaaccc gatgaagggc accaccggcg gtatcatcga
     6001 caaggagatg ccgatccacg tttccaacgt ggcggtgttc aacccgacca cccagaaagc
     6061 cgaccgcatc ggcgtcaagc agatggacga cggccgccgc gtgcggatct acaagtcgaa
     6121 cggcgaaatg ctggacgctt aagaggaaga catggcgaag gccaaggaaa cggcgaagaa
     6181 ggacacggca acggccaagg aagacatgcc gaaggccaag gacgacctgc ccaaggccaa
     6241 gaaagcggct cccgcgaagg ccgcgtccgc gaaggcggta tctgaaaagg ccgcttcggt
     6301 tcaaccggcg cggctgcggc agcattaccg cgaagcagtg gtgccggctc tgatgaagca
     6361 gttcggctac aagaccgcca tgcaggtgcc acgcatcgac aagatcgtgc tgaacatggg
     6421 cgtgggcgag gcggtcaacg acaagaagat tctcgacaac gcggtgggcg atatgaccag
     6481 gatcgccggc cagaaaccgg tggtcaccaa ggccaagaaa tcgatcgccg gcttcaagat
     6541 ccgcaaggac tatccgatcg gctgcatggt gacgttgcgc cgcgggcgca tgtacgaatt
     6601 cctggatcgg ctggtgtcca ttgcaattcc acgtatccgc gatttccggg gtatttcggc
     6661 acgttccttc gatggccggg gcaatttcaa ccttggcgtg aaggagcaga tcatctttcc
     6721 ggagatcgag tacgacaaga tcgacgtgct caggggtttg aacatcacga taaccacgac
     6781 agcgaagacc gacgatgaag cgcgtgcgct tctcgccgcc ttcaagttcc ctttcaagaa
     6841 ctgaggccgt catggcaaag accgctctga tcaatcgaga caagaagcgc cgcgtgacgg
     6901 tgaagaaatt tgccgtcagg cgcgccgagc tattcgcga
//
LOCUS       DASQOP010000333        14753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1379788, whole genome shotgun sequence.
ACCESSION   DASQOP010000333 DASQOP010000000
VERSION     DASQOP010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14753
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1379788"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="VE030_13010"
     CDS             <1..1128
                     /locus_tag="VE030_13010"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZF24371.1"
                     /translation="VQTDVRPSDAALERLAERLLAAANPVLIAGHELATRDALKEAAE
                     LAEVLGAPVYQQTVAYAAHFPSEHPAFLGALTRNQQQVRSALAPHDLLVCLGADVLRM
                     SVHSLVDPLPERMPVVQIGERDWELGKNYPAEIAIGACVRETLRALLPILRRKMSAGR
                     KTQAAQRLVEIAKNNWTAKRERLAKDMLAVAGAKPLDPRWLMMTIADALPKDAVVVEE
                     GLTSTFSLQSFLRFRDAQGFYGLASGGIGFAMAGAVGISVALPNRPVVAIVGDGSAMY
                     SIQALWTAANLKRPITYVICDNRSYRILKERLVSMRGTEKFVGMDLRDPEIDFVALSQ
                     SMGVPAKRISDAQDVVPALRSAIASGVPNLLDISVADGFGG"
     gene            complement(1418..2125)
                     /locus_tag="VE030_13015"
     CDS             complement(1418..2125)
                     /locus_tag="VE030_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24372.1"
                     /translation="MAVCAQKRDQIAVKAFCLSALCLAALIGGCAGALAPEWQSGAHV
                     ALEKFRQNYLEGNTRLAERYFAEAKLSISATGRLDLAARAELIRCALATASLSDDACA
                     EFEALRSYVTAAERAYAQFLAGHWQDLRAQDLPEPYRGIVTGGGEASLNREIGRIEDP
                     VSRLVAAGALFRAGRLSPVGVATAIDTASAQGYRRPLLAYLNVQAKLAEQAGDSAALE
                     TIRRRIELVSQPLPDGR"
     gene            complement(2086..3084)
                     /locus_tag="VE030_13020"
     CDS             complement(2086..3084)
                     /locus_tag="VE030_13020"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HZF24373.1"
                     /translation="MERAPVPQPRIRNLGFKVGLLLTLIPFIAIGLVLYALYARGVFE
                     SSQELALIAKDAEGVTEGMPIMFAGFPIGQVSSMALTDSGEVQIKVWIREKDARWLRT
                     SSVFTLERQFFGGAKIRVTTRKMNDPQLLPGAIVKLVGIDAAQDLPQMIARANAVLQN
                     IEEMIKPDSTLNRSLAHLNEVTERMAGEYGVLGSFAGSPENARQVMETVRGVSALIAS
                     LKGVASRAESMLTRADEKVFAQGGVMDEARKSIVQLNAILGDARDSLKKADAVLANAQ
                     SASADGKAAAANIKGATTDLAALRTEVDDSIRKVGVLIDEINRRWPFARKNEIKLP"
     gene            complement(3051..3524)
                     /locus_tag="VE030_13025"
     CDS             complement(3051..3524)
                     /locus_tag="VE030_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24374.1"
                     /translation="MAENQDKRKRVVMLAGNAERQRLIESHPEAGLVSETQPLRANLS
                     VLENITMVLQFRTNTYLGEANDIGWELLSLVGRTDCANKRDPDLDYEERFVAKLLRAV
                     VIAPPMIMIDRPAQLLPDTDYPAFLQRTLTALDQRFAQCWILDYTWNAPLYRSRG"
     gene            complement(3531..4325)
                     /locus_tag="VE030_13030"
     CDS             complement(3531..4325)
                     /locus_tag="VE030_13030"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZF24375.1"
                     /translation="MNALYDWLTQRGREWSYAWSRVFYFAALAVVGALSPSVYNRATR
                     QVVEKQIYFTAWQILPGFTLFVALFSCVFVQIVGATASDFGLYRYALELVIRILVLEI
                     LPLLTALFVAVRTGAAINTEVALMQIQNEFEALERVGVDPFRLELLPRVIGGTISVLV
                     LTLVNIVFALVIAHVMIFDFRPWNLPPGDFARVIGKVFDGPALLVLGVKILAFGLAVT
                     VIPISEGLNTPQKQIFYAPIAVLRGMVRLFFALMLIEIMAIAVIYV"
     gene            4537..5370
                     /locus_tag="VE030_13035"
     CDS             4537..5370
                     /locus_tag="VE030_13035"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24376.1"
                     /translation="MNKVRGLGDALANAASTIIERIVAYLPSVLGAILLLIVGWALAR
                     LLRALTMRAVLLLDKLLSRVGGPGGSERFRVGRASAVLGTVVFWVVLLFFIAAATQVL
                     GLQAFTDWLAHLIDYLPTLIAGVLIIAAGYIVSRFVADLLLATATRLAHTQRLALARV
                     AQVLILVGAILVGADQVGIKITFLAIFAAGAAATIVGGVALAVGFGARDYIANLIGAH
                     YMRQAFPVGQIIRVAGHEGRILEVTATVVIIETGEGRVTLPGRIYNEQPIAVISRQKN
                     G"
     gene            5363..6211
                     /locus_tag="VE030_13040"
     CDS             5363..6211
                     /locus_tag="VE030_13040"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZF24377.1"
                     /translation="MAEPEALTLTYIAAHPGEAARVLERIPGVDAAALFATVPARAGA
                     PVLTAMLPSAAARILGSLDDEPALGLLTAAGVQAAVTVLRYIPEPRRSRLLAGLPTAT
                     AVASRVLLGYPDDSVGAWADPDVIALAPETRVSDALERVRGGDEKKVEQVYAVDHDQR
                     LIGIVYLVDLLRVPDANTLAAVLHKPLAMLNAMTPLTGAATQRGWQQSSVLPVVERDG
                     RLIGVMHRATLARALARSRATTQPADEVTITGVLTRGYWDAASGLAEAGLWLLPPAKP
                     VLPEEP"
     gene            6208..7518
                     /locus_tag="VE030_13045"
     CDS             6208..7518
                     /locus_tag="VE030_13045"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HZF24378.1"
                     /translation="MNNGALPAILALNRRYFLDYPHEAARQLESLPPQEIADLLAGQA
                     PHAVVRAWQLLAPNVAGAALERVPEALARHLMSESEPAASVTVLAQFDRADRDRLLGM
                     LELQVASELREMLDYPEDSAGRLMDPSVTPIRSDLSIAEALGRLRTVKSRGLRELFVV
                     DEEMRLIGRVEIQDLAMAEQAQALREITRPLIAAVKDLDPREDVVKILQHQPITELPV
                     VNHSGRFVGVIRQAALVSAIESETTVDIQTMVGASRDERALSGPVFAVRKRLPWLQVN
                     LLTAFLAAAVVGLFESTIAKFTALAVLLPVVAGQSGNAGLQALAVTMRGLVLREISPR
                     QWPRMVWKEASVGLANGLAVAATTAVGVWFWSGSIGLVTVITSAMVTSMVIAGVSGAL
                     VPIMLRRFGQDPAQSSSIVLTTITDVAGFLSFLGIATLLSSMLG"
     gene            complement(7634..8419)
                     /locus_tag="VE030_13050"
     CDS             complement(7634..8419)
                     /locus_tag="VE030_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24379.1"
                     /translation="MKWIGWVPAVAAAFFVCVAQAEQDMSEEALKLDAVSRLIEAHDP
                     SLSVNIGRVYVKQAALGAARDLLYQKGRAAGLKKETWNLSSPQWQAAERELVQGTDAL
                     IQRRVANGNWVREAWSEQVATLLNGEEADEIAVHFQTEGGQLQRRVIEWFVGELTLQT
                     YTFTDRLKYGVPGSEQEMEDLRKTTYEIRSHFDEIYDLTKYPDAVRFASRGPGVEYFK
                     MMVLQGVHALHVHLETVANEARETVHFRSALVDPYIEKVKGER"
     gene            8694..9977
                     /locus_tag="VE030_13055"
     CDS             8694..9977
                     /locus_tag="VE030_13055"
                     /inference="COORDINATES: protein motif:HMM:NF027805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HZF24380.1"
                     /translation="MATDNHTLVVLDPGHFHAALTLRQRHPRLSDEVHVYAADGPDLE
                     TFLRIVCTFNQRADNPTRWNLHVYRGEDRFERLLSERRGDVVIIAGRNDSKMTSIHRL
                     HAGGFFVLGDKPWLIDPAQIGMLEEAAATPPLAMDIMTERHEIATRLQVALAAQPRVF
                     GRLRNDGDQPAIFMQSVHHLYKIVNGVPLVRPPWVFDVAVQGEGITDVTTHLVDLAQW
                     ITGGGQPFDYGPDVELLSARQWPTEVPRDMFTRITGLDDFPWALRHRIADGMLSVLCN
                     AAMSFRLRGIAAAIESRWALAVPEGGGDTHYALVRGTAADLIVEHSGLTHYVTELTVR
                     PVDGSAGYAAALERAIESLEAEFPGLGIEPSAGEFRIAIPQALRTTHEQHFAKVLDQF
                     LSRIDGGDLSDDYRRDLVAKYRLLMRAAELSLCNA"
     gene            10158..11393
                     /locus_tag="VE030_13060"
     CDS             10158..11393
                     /locus_tag="VE030_13060"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZF24381.1"
                     /translation="MKLGFIGAGFIARFHAIAIHQVRGLEISGLIRRRGAEALAGFCR
                     EHKLGEAKLFDSIAEMAKHVDVLALYVPNYARIEVMEQVAAAVKAGAKLKGVILDKPL
                     GRNMKEARRLVELAREAKLPTAYFENQIFMKAIRTQRNQLLPVTRSMGPLALTRSAEE
                     HGGPHEPWFWDPTRQGGGVLLDMGCHSIAVGRYVLTPPDKDLLFLQPLSVSADLGLLK
                     WGQKKWREQLLRDRGVDYSRTPAEDFATGMITYRNPETGQIVKSQFTDSWMFEKQGLR
                     LFMDGMGPGYAFEINTLVSPLNVFIGDVAAQAIADQESALEKATASRGLLAVQYNEAD
                     LYGYTDENVDMLEAFSAGRSAMLSWEYGLEVQRLVMASYLSAEKSRTIDLTDKATIAE
                     LDAYVPLIQQGKGGAQLLR"
     gene            11480..11911
                     /locus_tag="VE030_13065"
     CDS             11480..11911
                     /locus_tag="VE030_13065"
                     /inference="COORDINATES: protein motif:HMM:NF037472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rap1a/Tai family immunity protein"
                     /protein_id="HZF24382.1"
                     /translation="MRHWSFALRGLLLASALIAGALLAAVPAGAGPFLQGKFTGEEML
                     AHCKAADKDPIQDFGRGICTGFIDGFVAGHFVAETWHGFHHRDEKMDDIFGHLCVPDG
                     TTRGQLARIFVQFLEKNPEKRKLPAGLLLEDAFRESFPCPK"
     gene            11960..12283
                     /locus_tag="VE030_13070"
     CDS             11960..12283
                     /locus_tag="VE030_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24383.1"
                     /translation="MPTVDAEQYVRVMEGAREDVYVVAARRSLDVDSISVQFDRGRGS
                     GPHQEHVLAISTRDNAVSVEKSGIPHEWIATLGTGYIDTRFVKCVAVLLAKLEKKAAE
                     AGIRL"
     gene            12398..12622
                     /locus_tag="VE030_13075"
     CDS             12398..12622
                     /locus_tag="VE030_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HZF24384.1"
                     /translation="MAKKNAGEQIYKVIEVIGTSTKSWEDAATNAVETAAKSLRDLRI
                     AEVVKMDMKVDGGKVVAFRTRVLVSFKYDA"
     gene            12832..13143
                     /locus_tag="VE030_13080"
     CDS             12832..13143
                     /locus_tag="VE030_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24385.1"
                     /translation="MPAIGRLVCSQRTAPALYLSLCTLWQNEFRFRMTLVGGVVFLIP
                     CFGVADMAKKLSPEEAAVMIIRRRLQQVETAKKESRRSDEPEKPRAAKAQPPKASRAG
                     R"
     gene            13270..13686
                     /locus_tag="VE030_13085"
     CDS             13270..13686
                     /locus_tag="VE030_13085"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZF24386.1"
                     /translation="MNKIFIHDQLIRFSHCDPAGIVYFPRFFDLAHATMEDWFASGVN
                     HPLPDLIRDRHIGTPTVSIQCDFALPLRMGDTLRFELRVPKLGNASVHLAYSGKKDGV
                     EHLTIRQTIVFTALDAGRAVSIPEDLRPRIEEYLVR"
     gene            13837..14460
                     /locus_tag="VE030_13090"
     CDS             13837..14460
                     /locus_tag="VE030_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HZF24387.1"
                     /translation="MNTRIAARPNRSSLSKFALACLLWPALALAHVGQGDISGGFLAG
                     VAHPVLGPDHVVAMVAVGIWGAQLGAPAIWVLPVTFPIVMAFGGVLGGLGIPIPGIEI
                     GIALSAIVLGGMIAIARRPPLWVAAIVVGVFAIFHGHAHGAELPESANAISYSVGFVA
                     ATGSLHALGILIGVANRWSAGAKVLRAGGGMIAACGFYFLTGHLIAG"
BASE COUNT         2722 a         4688 c         4616 g         2727 t
ORIGIN      
        1 gtgcagaccg acgtgcggcc ttcggacgcc gcgctggaac ggctcgcgga gcggctgctc
       61 gccgcggcca atccggtgtt gatcgccggc cacgaacttg ccacgcgcga cgcgctgaag
      121 gaagcggcag aacttgccga agtcttgggc gcgccggtgt accagcagac ggtcgcctac
      181 gccgcacact tcccgtccga gcatccggcc ttcctcggcg cgctcacccg caaccagcag
      241 caggtacgtt cggctctggc cccccacgac ctgctggtgt gcctgggcgc cgatgtgctg
      301 cgcatgtcgg tgcacagtct cgtcgatccg ctgcccgagc gcatgccggt ggtgcagatc
      361 ggggagcgcg actgggagct gggcaagaat tatccggccg agatcgccat cggcgcctgc
      421 gtcagggaaa cgctgcgtgc gctgctgccg atcctgcgca ggaaaatgag tgccgggcgc
      481 aagacgcagg cggcgcaacg gctggtcgaa attgcgaaga acaactggac cgcaaagcgc
      541 gagcgcctgg ccaaggacat gctggccgtc gcgggggcaa aaccgctgga tccgcgctgg
      601 ctgatgatga ccatcgccga tgcgctgccg aaagacgcgg tggtggtcga ggaaggcctg
      661 acctccacgt tctcgttaca gagttttctg cgctttcgcg atgcgcaggg tttctacggc
      721 ctggcttccg gcggcatcgg ctttgcgatg gccggcgcag tcggcatcag cgtggcgctg
      781 ccgaaccggc cggtggtcgc catcgtcggc gacggcagcg cgatgtacag catccaggcg
      841 ctgtggaccg cagccaacct caagcgcccg atcacctacg tgatctgcga caaccgcagc
      901 taccgcatcc tcaaggaaag gctggtgtcg atgcgtggca ccgagaaatt cgtcggcatg
      961 gatctgcgcg atccggagat cgacttcgtc gcgctgtcgc agtcgatggg cgtaccggcc
     1021 aaacgcatct ctgatgctca ggacgtagtg ccggcgctgc gctcggcaat cgcgagcgga
     1081 gttccgaatc ttctggatat cagtgtcgcg gacggtttcg gcggctagag caatactcac
     1141 cacggaggac gcggaggaca cggaggaaga tcaatttcaa tagcaaagac aaaagggata
     1201 ctgataccgg cgcagtgctt ttcgtagtgg cgcagaaata ctctggcatc aatgaccagt
     1261 aatatcatca gatatgcgaa cagacagaag ttggcgtccg gacacggctt gcaggatcaa
     1321 gtctcatcca caacaaatga ttcggggatt tgcgagttct tcctcttgtt gttccgcttt
     1381 gattcctcgg tgtcctccgt ggtgagaaac gtatctctta tctgccgtcg ggcaacggct
     1441 gagacaccag ttcaatgcgc ctgcggatcg tttccagtgc cgcggagtcg ccggcctgtt
     1501 cggccagttt cgcctgcacg ttaagatagg cgagcagcgg ccggcggtat ccctgcgccg
     1561 aggcggtgtc gatggccgtc gcgacgccta caggtgaaag ccggccggcg cggaacagcg
     1621 cgcccgccgc caccagccgc gacaccgggt cttcgattcg accgatctcc cggttcaatg
     1681 acgcttcgcc gcctcccgtc actatgccgc gatacggttc cggcaaatcc tgggcacgca
     1741 ggtcctgcca gtgaccggcc aggaattgcg cgtacgccct ctccgccgct gtcacataac
     1801 tccgcagcgc ctcgaactcc gcacaagcat cgtcactaag cgacgcggtg gcaagagcgc
     1861 aacgtatcag ttcggcgcgc gcggccaggt ccagccggcc ggtggcggag atgctcaact
     1921 tcgcttcggc gaagtagcgc tcggccaggc gcgtgttgcc ttccaagtag ttctgcctga
     1981 atttctccag cgcaacatgt gccccggact gccattccgg cgccagcgcg ccggcgcacc
     2041 cgccaatcag cgcggccaaa catagcgcgg acaggcaaaa cgccttcacg gcaatttgat
     2101 ctcgtttttg cgcgcaaacg gccatctgcg gttgatctcg tcgatcagga cgccgacctt
     2161 gcggatgctg tcgtcgactt cggtgcgcag cgcggcaaga tcggtggtcg cgcccttgat
     2221 gttggcggcg gccgccttgc cgtcggcgct cgccgactgc gcgttggcga ggacggcgtc
     2281 ggcctttttc aggctgtcgc gcgcgtcgcc gaggatggca ttgagctgga cgatggattt
     2341 acgcgcttca tccatgacgc ctccctgggc aaacaccttc tcgtcggccc tggtcagcat
     2401 gctttccgcg cggctggcga cgccctttag cgaagcgatc agcgcgctca cgcccctgac
     2461 tgtttccatc acctgtcttg cattctcggg actgcccgcg aagctgccga gcacgccata
     2521 ctcgccggcc atgcgctcgg tcacctcgtt cagatgcgcc aggctgcggt tgagggtgga
     2581 gtcgggtttg atcatctcct cgatgttctg caacacggca ttggcgcggg cgatcatctg
     2641 gggaagatcc tgcgccgcgt cgatgccgac cagtttgacg atcgcgccgg ggagtagttg
     2701 cgggtcgttc atcttgcgag tcgtgacccg gatttttgct ccaccgaaaa actgcctttc
     2761 cagggtgaat acactggaag tgcgcagcca gcgcgcgtcc ttctcgcgta tccacacctt
     2821 gatctggact tcaccgctgt cggtgagcgc catgctgctc acctggccga tcgggaatcc
     2881 cgcaaacatg atgggcatgc cctcggtcac gccttcggca tccttggcga tcagtgccag
     2941 ttcctgcgag ctttcgaaca cgccgcgggc atagagtgcg tacagcacca gcccgattgc
     3001 tatgaacggg atcagggtga gcaggaggcc caccttgaat cccaggttcc ttatccgcgg
     3061 ctgcggtaca ggggcgcgtt ccatgtgtaa tccagaatcc agcactgggc gaagcgttga
     3121 tcgagcgcgg tgagcgtgcg ttgcaggaag gccggatagt cggtgtccgg caacagctgc
     3181 gccgggcgat cgatcatgat catgggcgga gcaatcacca ccgcccgcag cagtttcgct
     3241 acgaaacgct cctcatagtc caggtcgggg tcgcgcttgt tggcgcaatc ggtgcgcccg
     3301 accaacgaca aaagttccca gccgatgtcg ttggcctcgc ccaggtaggt gttggtgcgg
     3361 aactgcagca ccatggtgat attttccagc accgacaggt tggcccgcag cggctgggtt
     3421 tccgacacaa gtcccgcctc gggatgcgac tcgatcagcc gctggcgttc ggcattgccg
     3481 gccagcatga ccacccgctt gcgcttgtcc tggttctccg ccatgggttt ctatacgtag
     3541 atcacggcga tggccatgat ttcgattaac atcagcgcaa aaaacaaccg caccatcccg
     3601 cgcagcacgg cgatgggcgc gtagaaaatc tgcttctgcg gcgtgttcag gccctcggaa
     3661 atcggaatga ccgtcactgc cagtccgaat gccaggatct tgacccccag caccagcagc
     3721 gcaggcccgt cgaacacctt gccgatcacc cgggcgaaat cccccggcgg caggttccac
     3781 ggacgaaagt cgaagatcat cacgtgcgcg atgaccagcg cgaacacgat gtttaccagc
     3841 gtcagcacca gtaccgagat cgtgccgccg atgacgcggg gcagcagttc cagcctgaac
     3901 ggatcgacgc cgactctttc cagcgcttcg aactcgttct gtatctgcat cagcgcaacc
     3961 tcggtgttga tcgcggcgcc ggtgcgtacc gccacgaaca gcgcggtaag cagcggcaga
     4021 atctccagta ccaggatgcg gatgaccagc tcgagcgcat agcggtacag cccgaagtcg
     4081 ctggcggtcg ccccgacgat ctgaacgaac acgcagctga acagcgccac gaaaagggtg
     4141 aaaccgggaa gaatctgcca cgcggtgaaa tagatctgct tctccaccac ctggcgcgtg
     4201 gccctgttgt agaccgaggg cgacagcgcg ccgaccaccg cgagcgcggc gaagtagaac
     4261 acgcggctcc acgcatagct ccattcccgc ccgcgctgcg tcagccagtc gtacagggca
     4321 ttcatcgccg aatgataccg cggagtacgc gccgccggcc tgccggtggc ggaaacttcg
     4381 aaacggcatt gccgaccgcg aaaaaaaccg ccgagccatc acaacggcca ccggcagttg
     4441 ccgcggattg gcgcttccct tgcgccgagc agcagtgcta agctcgcgac gacgccgcgg
     4501 cggtccgcgg cacacgtttt cctcggagca ctgcggatga acaaagtacg cggtctcggc
     4561 gacgctctgg ctaacgctgc ctcgaccatc atcgagcgta tcgtcgccta tctccccagc
     4621 gtcctcggcg cgatcctgct gctcatagtc ggctgggcgc tcgcgcgcct gctgcgtgcg
     4681 ctgaccatgc gcgcggtgct gctgctagac aagcttctct cccgcgtcgg cgggcctgga
     4741 ggtagcgagc gcttccgcgt cgggcgcgcg tccgccgtgc taggcaccgt agtcttctgg
     4801 gtagtattgc tattcttcat tgccgcggca acgcaggttc tcggactgca ggcgttcacc
     4861 gactggctgg cacacctgat cgactatttg ccgacgctga tcgccggcgt gctcatcatc
     4921 gcggccggct acatcgtttc ccgtttcgtt gccgacctgc tcctggccac cgcaacgcgg
     4981 ctggcgcaca cgcagcgcct ggccctggcg cgggttgcac aggtgctgat cctggtcggc
     5041 gccatcctgg tgggcgccga ccaggtcggc atcaagatca ccttcctcgc catattcgcc
     5101 gccggcgctg ctgcaaccat cgtcggtggc gtcgccctcg cggtaggatt cggcgcgcgc
     5161 gactacatcg ccaacctcat cggcgcgcac tacatgcgcc aggccttccc ggtcggccag
     5221 atcatccgtg tcgccggcca tgaaggccgc atcctggaag tgaccgccac tgtggtcata
     5281 atcgagaccg gtgaaggccg cgtcacgctg cctggacgca tctacaacga gcagccgatc
     5341 gcagtgattt cgcgccagaa aaatggctga acccgaagcg ctgacgctca cgtatatcgc
     5401 cgcgcacccc ggggaggcgg cgcgggttct ggaacgcatt ccaggggtcg atgccgcggc
     5461 gcttttcgca accgttccgg cacgcgccgg agcgcccgta ctgaccgcaa tgctgccatc
     5521 ggcggcggcg aggatcctcg gttcgctcga cgacgagccc gcgctcgggt tgctcaccgc
     5581 ggccggcgtg caggcggcgg tgaccgtgct gcgttacata cctgagccgc gcaggtcacg
     5641 cctgctcgcg ggactgccca ccgccaccgc cgtggcctcg cgcgtactgc tcggttaccc
     5701 ggacgattcg gtcggcgcgt gggccgaccc cgacgtcatc gcactcgcgc ccgagaccag
     5761 ggtaagcgac gcgctggaac gcgtacgtgg cggcgacgaa aagaaagtgg agcaggtata
     5821 cgcggtcgat cacgatcagc gcctgatcgg catcgtgtat ctggttgatc ttctgcgcgt
     5881 gcccgatgcg aatacgctcg cagccgtcct gcacaagccc ctggcgatgc tcaacgcaat
     5941 gacgccgctc accggagcgg cgacccagcg cggctggcag cagtccagtg tgctcccagt
     6001 ggtggagcgt gacggacggc tgatcggcgt gatgcaccgt gcgacgctgg cccgtgccct
     6061 tgcacgcagc cgggcgacca cccagcccgc cgacgaagtc accattaccg gcgtgctcac
     6121 ccgcggctac tgggacgcgg cctccgggct agcggaagcc gggctgtggt tgctgccgcc
     6181 ggccaaaccg gtgctgccgg aagagccatg aacaacgggg cgttgccggc catactggcg
     6241 ctgaaccggc gctatttcct cgattacccg cacgaggcgg cgcgacagct cgagtccctg
     6301 ccgccgcagg agatcgccga cttgctggcc gggcaggcac cgcatgcggt agtgcgcgca
     6361 tggcaactgc tcgcaccgaa tgttgccggc gcggcactcg agcgcgttcc tgaggcgctg
     6421 gcgcgccacc tcatgtccga atccgagcct gcggcaagcg tgaccgtgct tgcccagttc
     6481 gaccgcgctg atcgcgatcg cctgctcggg atgctcgaac tacaggttgc cagcgagctg
     6541 cgcgaaatgc tggactaccc ggaggattcc gccggccggc tgatggaccc cagcgtcact
     6601 ccgatccgct ccgatctctc gattgccgag gcgctggggc gcctgcgcac ggtcaagagc
     6661 cgcgggctgc gggaactctt cgtcgtggac gaggaaatgc gtctgatcgg acgcgtcgag
     6721 attcaggatc tcgccatggc ggagcaggcg caggccttgc gcgagatcac ccgcccgctc
     6781 atcgccgcgg tgaaagatct cgacccgcgc gaagacgtcg tgaagatcct gcagcatcag
     6841 ccgatcaccg aactcccggt ggtgaatcac tccgggcgtt ttgtaggcgt gatccggcag
     6901 gcggcgctgg tctcggcgat cgaatcggaa acgaccgtcg acatccagac catggtcggc
     6961 gcaagccgcg acgagcgcgc gctctccggg ccggtattcg cagtacgcaa acgcctgccg
     7021 tggctgcagg tcaatctgct caccgctttc ctggcggcgg cggtggtcgg tctgttcgag
     7081 agcacgatcg cgaaattcac cgcactcgcg gtattgctgc cggtggtcgc gggacagtcg
     7141 ggcaacgccg gcctgcaggc gctggcggtg acgatgcggg ggctggttct gcgcgaaatc
     7201 agtccgcgcc agtggccgcg gatggtatgg aaggaggcca gcgtgggact tgccaacggt
     7261 cttgcagtcg ccgcgactac cgcggtcggc gtgtggttct ggagcggctc gatcggcctg
     7321 gtcacggtca ttaccagtgc gatggtgacc tcgatggtga tcgccggcgt gtcgggcgca
     7381 ctggtaccga tcatgctgcg ccgtttcggc caggatcccg cgcagtcatc gtccatcgtg
     7441 ctgaccacta tcaccgacgt ggccggcttc ctttccttcc tcgggatcgc gacgctgctg
     7501 tcgtcgatgc tgggctgaat tcgaggggtg agggacgagt ggcgaggggc gaggggaaag
     7561 agaaaatcaa aggcagaact gaaaagcagg ctgaaggtag aaggacttaa ggaagcaaga
     7621 gggtctccca cttttacctt tcacctttca ccttttcgat gtatggatcc accagcgctg
     7681 agcggaaatg aaccgtctcc cgcgcttcgt tggcgaccgt ttccaggtgc acgtgcaggg
     7741 cgtggacacc ttgcagcacc atcatcttga agtactccac ccccggaccg cggctggcga
     7801 aacgcactgc gtcgggatac ttggtgaggt cgtagatttc atcgaagtgg ctgcggatct
     7861 cgtaggtggt cttgcgcagg tcttccatct cctgctccga acccgggacg ccgtacttga
     7921 ggcgatcagt gaaggtgtag gtctgcagcg tcagctcgcc cacgaaccat tcgatcaccc
     7981 gccgctgcaa ctggccaccc tcggtctgga agtgcaccgc gatctcgtca gcctcttcgc
     8041 cgttgagcag tgtggcgacc tgctcggacc aggcctcccg aacccagttc ccgttcgcaa
     8101 ccctgcgctg gatcaaggca tccgtgcctt gcacgagttc ccgctcagcg gcctgccatt
     8161 gcggtgaaga aagattccag gtctccttct tcaaccccgc ggcccggccc ttctggtaca
     8221 acagatcacg cgcggcgccg agcgccgcct gcttgacgta gacccgcccg atattcaccg
     8281 aaagcgacgg gtcatgcgct tcgatcagcc tcgataccgc gtcgagcttc agcgcctcct
     8341 cggacatgtc ctgttcggcc tgcgccacgc acacaaaaaa ggctgcggcc acggcgggaa
     8401 cccaaccaat ccacttcacc tgaacctccc gaaaatgcca atgcacttcc attgcgtgag
     8461 gacgcgcgcc aacagttccc cctcacgttt tgagcggcga taccgcggcg atcaaactgc
     8521 gcattttagg ccaatgaccg cagatccgat ctagaacgaa tatgctgttt cgtgacggtt
     8581 ctttgcgccg gcaccccgcg gggttagcat tgtgccgaca acctgcggga aggcgcgggc
     8641 ctgacgcttg cgtgcgcccg ctccggagca aagcagaaaa gaatcgaaag aaattggcaa
     8701 cagacaatca cactctggtg gttcttgatc cggggcattt tcacgccgcg ctcactctgc
     8761 gccagcgcca tccacggctc agcgacgaag tgcacgtcta tgccgcggac ggcccggacc
     8821 tggagacttt cctgcgcatt gtgtgcacct ttaaccagcg cgccgacaac ccgacgcgct
     8881 ggaacctcca cgtatatcgc ggcgaggacc gttttgaacg cctgctctcc gaaaggcgcg
     8941 gtgacgtcgt gatcatcgcc ggaaggaacg acagcaagat gacatcgatc caccgcctgc
     9001 acgcgggcgg cttcttcgtg ctcggcgaca agccctggct gatcgatccg gcccagatcg
     9061 ggatgctgga ggaagcggcg gctacgccgc cactggcgat ggacatcatg accgaacggc
     9121 acgaaatcgc gacccggttg caggtggcgc tcgccgcaca gccccgggta ttcggccgcc
     9181 tgcgcaacga tggcgatcag cccgctattt tcatgcagag cgtccaccac ctttacaaga
     9241 tcgtcaacgg cgtgccgctg gtgcggccac cgtgggtttt cgatgtcgcc gtgcagggtg
     9301 aaggcatcac cgatgtcacg acgcacctgg tcgatctcgc gcaatggatc accggcggcg
     9361 ggcagccctt cgattacggc cccgacgtcg aactgctgtc cgcgcggcaa tggccaacag
     9421 aggtgccgcg cgacatgttc acgcgcatta ccggcctcga tgacttcccg tgggcgctgc
     9481 gccatcggat cgccgacggc atgctttccg ttctctgcaa tgcggcgatg agcttccggt
     9541 tacgcggcat tgcagctgca atcgagtccc ggtgggcgct cgcagttccg gagggcggcg
     9601 gggacacgca ctatgccctc gtacgcggaa cggccgccga cctgattgtc gagcatagcg
     9661 ggctgacgca ctatgttacg gaactcacgg tccggcctgt cgatggcagc gcgggttacg
     9721 cagccgcact cgagagagcg atcgaatcgc tcgaggctga atttccgggg ctgggaatcg
     9781 aaccctcggc cggcgagttc cgcatcgcca ttccgcaggc gttgcggacc acgcacgaac
     9841 agcacttcgc caaagtgctc gaccagtttc tcagccgcat agacggcggc gacttgtcag
     9901 acgattaccg acgcgacctg gtagccaagt acaggctgct catgcgcgcc gcggaattgt
     9961 ccctctgcaa cgcctgacgg cccgcgcgcc gtcgcccggc ctcccggatc catttcgatc
    10021 ttttctaccc ggcgccgtgg ggttagcatg gcaagataac aactgtagct ggtccctcgt
    10081 aaacgcgttt tgcctcggat actttccgcg accagccact agccactagc caccagccac
    10141 tgattttggg gaaggaaatg aaactcggat tcatcggggc aggcttcatc gcccgcttcc
    10201 acgcgattgc cattcatcag gtacgcgggc tcgagatctc cggcctgatc aggcgccgcg
    10261 gcgccgaggc gctggcgggc ttctgccgcg agcacaagct gggcgaggcg aagctcttcg
    10321 acagcattgc cgagatggcg aagcacgtcg atgtccttgc gctctatgta cccaactacg
    10381 cccgcatcga agtcatggag caggtcgccg ccgcggtcaa agccggcgcg aagctcaagg
    10441 gcgtgatcct ggacaagccg ctcggccgca acatgaagga agcccggcgt ctggtcgagc
    10501 tggcgcgcga ggcaaaactg ccgaccgctt acttcgagaa ccagattttc atgaaagcga
    10561 tccgcaccca gcgcaaccag ctcctgccgg tgacgcgctc gatggggccg ctggcgctca
    10621 cgcgctccgc agaagagcac ggcggtccgc acgaaccgtg gttctgggat ccgacccgcc
    10681 agggcggcgg cgtgctgctg gacatgggct gtcacagcat cgcggttggc cgctatgtgc
    10741 tcaccccgcc cgacaaggac ctgctgttcc tgcaaccgct ctcggtcagc gccgacctcg
    10801 gcctgctcaa gtggggccag aagaagtggc gcgagcaatt gctcagggat cgcggcgtcg
    10861 attattccag gaccccggcg gaggacttcg ccaccgggat gatcacctac cgcaatcctg
    10921 aaaccgggca gatcgtgaag tcgcaattca ctgactcgtg gatgttcgag aaacaggggt
    10981 tgcggctgtt catggacggc atggggccgg gctacgcatt cgagatcaac acgctggtct
    11041 ctcccctcaa cgtgttcatc ggcgacgtgg ccgcgcaggc gatcgccgac caggaaagcg
    11101 cactggaaaa ggctaccgcc agccgcgggc tgctggcggt gcagtacaac gaagccgatc
    11161 tctacggcta caccgacgag aatgtcgaca tgctggaggc gttctcggcc ggccgcagtg
    11221 cgatgctctc gtgggaatat ggcctggagg tccagcggct ggtgatggcg agctacctgt
    11281 cggccgagaa aagcaggacc atcgacctca ccgataaggc cacgattgcg gaactggatg
    11341 cctacgtccc gttgatacaa cagggcaagg gcggcgcaca attattgcga tgaatacgca
    11401 agcgcaaggc gacaggttcc ctttactgcg tgaccggatt gctccggccc gcgctctcgt
    11461 ggatagtcgc ggacatttga tgcgccattg gtcttttgcg ttgcggggcc tgctcctcgc
    11521 gtcggccctg atcgccggcg cgctgttggc ggccgtgccg gccggcgccg gcccgttctt
    11581 gcagggcaag tttaccggcg aggagatgct cgcgcactgc aaggcggcgg acaaggaccc
    11641 gatacaggac ttcggcaggg gcatctgcac tggattcata gacggcttcg tcgccggtca
    11701 cttcgtcgcg gaaacctggc acgggtttca ccaccgcgac gaaaagatgg acgatatttt
    11761 cggacatctg tgcgtaccgg atggcacgac gcgaggccag ctggcccgga ttttcgttca
    11821 gttcctggaa aagaatccgg aaaaacgtaa actgccggcc gggctgctgc tggaagatgc
    11881 gtttcgggag tcgtttcctt gtccgaaatg agccgctgtt ccaatatctt ttgagttcca
    11941 gccactggga gcacacctga tgccgacggt ggatgcggag cagtacgtcc gcgtcatgga
    12001 aggggcccgc gaagacgtct acgtcgtagc ggcgcggcgc agcctggacg tggactccat
    12061 cagcgtgcag ttcgatcgcg gccgcggttc cgggccgcac caggagcatg tattggcgat
    12121 ctctacccgc gacaacgccg tgtcggtcga aaagtctggc attccccacg aatggatcgc
    12181 cacgctaggc acgggctaca tcgacacccg attcgtgaaa tgcgtggccg tactgctggc
    12241 gaagctggaa aaaaaagcag ccgaagcggg tattcgtctt tagggatcaa ggccgaggtt
    12301 gcggatgggt catgacgtcc gcaatcatgc ggtgtagcat aggctcacgt cgcggcgatg
    12361 tgtgttcgcg agcgacatca caagggggag gaatgtcatg gctaagaaga acgcaggtga
    12421 acagatctac aaagttatcg aagtgattgg aaccagcacc aagtcctggg aagacgccgc
    12481 gaccaacgcc gtggaaaccg ccgccaagag cctgcgcgat ctgcgcatcg cagaagtggt
    12541 gaaaatggac atgaaggtcg atggcggcaa agtcgtcgca ttccgcaccc gggtcctggt
    12601 gtccttcaag tacgacgcct gatgcaaacg atcgcccgcg ttattgtttt tcggaaaaat
    12661 caggcggcca agggccggca ctaaaagcgt tgaaaacgag cccagcgagt ttcagtgcag
    12721 actaacccgt ttgcgggtta tgtcggaact acaattccat catcgcattt gattttcgca
    12781 gagaacggca agaaaggacg caaagaaatt cgatagtcct gaacgctctt aatgccggcc
    12841 atcggccggc tggtttgttc gcagcgcaca gcacctgccc tttacctttc tctgtgtact
    12901 ctgtggcaaa atgagtttcg tttccgcatg acactggtcg gcggcgttgt ctttctgatc
    12961 ccctgctttg gagttgccga tatggccaag aaactgtcgc ccgaagaagc cgccgtgatg
    13021 atcatccggc gccggcttca gcaagtcgag acagcgaaaa aggagtcacg gcgcagcgac
    13081 gaaccggaga agccgcgcgc agccaaggcg cagccgccta aagcttcccg cgccgggcgc
    13141 tgagggcaca cccgctggca aggcccgcac cggccgtgca ggggaagttc gatcagggct
    13201 tccccaatgc ttcccggccc ggctttacag aatcggtccc ccagccgcat ctgatcgggc
    13261 gatcacgccg tgaacaaaat attcatccac gaccagttga tccggttctc ccactgcgac
    13321 cccgccggca tcgtttactt cccgcgtttc ttcgatctcg cgcatgcgac gatggaggac
    13381 tggttcgcca gcggcgtcaa ccatccgctg cccgatctca tccgcgaccg gcatatcgga
    13441 acgccgacgg tgagcatcca gtgcgacttc gcgttgccgc tcaggatggg agacacgctg
    13501 cgtttcgagt tgcgggtgcc caaattgggc aacgcctcgg tacaccttgc ctacagcggc
    13561 aagaaggacg gcgtcgaaca tctcacgatc cggcagacca tcgtcttcac ggcgctcgac
    13621 gccggacgtg ccgtgtcgat ccccgaagat ctgcggccgc gcatcgaaga atatctggtt
    13681 cgttgatcgg cgtcagccgg ccgatggccg gccactaaat cgttgacgca aaggacgcga
    13741 agaacggcaa ggatgaagga atagcaagag gcgggttccg ggtgctaagt gctgtctgct
    13801 actcttaagc catcggtcgg tcgggaagga acagacatga acacaagaat cgctgctcgt
    13861 cccaatcgat cctcgttgag caagtttgcg ctggcctgtc ttttgtggcc ggcgctggca
    13921 ctggcacacg taggccaggg cgacatctcg ggcggctttc tcgccggcgt ggcgcacccg
    13981 gtactcggac ccgatcacgt ggtggcgatg gtggcggtag gcatttgggg cgctcagctc
    14041 ggcgcgccgg caatctgggt gcttccggtg accttcccga tcgtcatggc tttcggcggc
    14101 gtgctgggtg ggctcggcat tccaataccc gggatcgaaa taggcattgc cctttccgcg
    14161 atcgtgctcg gcggcatgat cgcgattgcc aggcgcccgc cgctttgggt tgccgcaatt
    14221 gtcgtcgggg tttttgcaat cttccatggt cacgcccacg gcgcggaatt gccggagtcg
    14281 gccaacgcga tttcctactc ggtcggattc gtggccgcga ccggcagcct ccacgccctc
    14341 ggcatcctca tcggcgtggc caatcgatgg agcgccggcg cgaaggtctt gcgtgccggc
    14401 ggcggcatga tcgccgcgtg cggattctat ttcctgacgg gacatttgat cgccggctag
    14461 tagtgtcctg ttccgccaat tggtgggata acttgtatac cttggaaggc agacctcata
    14521 tccatttggc aactatccat catcgatcgg aaaacccgcg aaatcggcgt caggcaaggc
    14581 gcgagccctt gacagtggcg ggcaccacgg caaggactcg taacacagca tggcgccgat
    14641 tctcgtcggg ttttcagccg cgaattggcg gaacaggaca ctaagtcatc atgcctgccc
    14701 gcctttcgat gccggccgtg agagccggcg tcgcactgtg cctgctgctc gcg
//
LOCUS       DASQOP010000334         5779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_977615, whole genome shotgun sequence.
ACCESSION   DASQOP010000334 DASQOP010000000
VERSION     DASQOP010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977615"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VE030_13095"
     CDS             complement(<1..278)
                     /locus_tag="VE030_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HZF24388.1"
                     /translation="MLRPCALCGGLFLAAAAFAAGDDGEPTRQLEFVPPPAGSYRLEH
                     IMTAPDGTVLDTDDQPIPLARFTTGKVTVLSFMYTSCSDAWGCPFAYQV"
     gene            complement(278..1615)
                     /locus_tag="VE030_13100"
     CDS             complement(278..1615)
                     /locus_tag="VE030_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008430 - selenium binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-binding protein SBP56-related protein"
                     /protein_id="HZF24389.1"
                     /translation="MMLRLSRVPRSLATALLAAALLGAGPYAGADETCNSPYIGNLIK
                     GQEDYVYVWTLGVQGLGDGSDKLVTIDVNPASKKYGKAIHSISVGARGEAHHMGFTDD
                     RRHLWAGGLSNSRIYVFDVGSNPAKPKLTKVIADLPERTGYAGPHTFYALPGRMLVGT
                     LSNTRDKGGVTGLALFNNKGDLISKYDLPTDNGGDGYGYDIGINPRKNLLLTSSFTGF
                     NNYMRDLGEVVADAEAMKKFGNTMVLWDLKAMKPKKVLSVPGAPLEIRWSLREGENWA
                     VVATALTSKIWLIREGANGEWEAKDVATIGDPAKIPLPVDISISRDGKGLWVNTFMDG
                     KTRYFDLSNPEAPKQTYEKVTGKQVNMVSQSWDGKRVYVTSSLLEKWDKKGTDNEQFL
                     RAFNWDGKELRPAFEVDFTKEKLGRAHHMKLGSKSLKSAFGDAEPPGEIAAAR"
     gene            complement(1722..3179)
                     /gene="dacB"
                     /locus_tag="VE030_13105"
     CDS             complement(1722..3179)
                     /gene="dacB"
                     /locus_tag="VE030_13105"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HZF24390.1"
                     /translation="MRIGERNAMSALGRLPLLLLLATAAQAMATTPMSEPPGPVAAAL
                     AQAGVPESSVGIYVHDLANNLPVVTVGIDRSLNPASVIKLVTTFAALEILGPAYTWKT
                     EAYLRGTLTGDRLNGDLVLKGYGDPKLTLENFWLFVRDLRNRGVREITGDLVLDRSFF
                     AIDDHDPAQFDNEPTRPYNVGADALLLNFKSFRLRFVPDEEKRAVAVYSEPVLPQVMV
                     VNNLYLAPGFCDVWPDKPARDGNTLTFFGTFPSSCGEKSRSFSMLTPNEYMAAVFGQF
                     WLQAGGSWSGRVREAEVPTNARLLSSWESAALAESIRDINKFSNNVMARNVFLTLGAQ
                     DGKPATLEKSDRAVSDWLARRGLSFPEMRIENGAGLSRTTRISARQLGELLIAAWNSP
                     LMPEYVASLPLAAVDGTMRRRLNNSPVAGQAHVKTGYLDGVRAISGYVMDSRGRMLVV
                     VFLINHPGARNAQAAVDALLEWVQEGSQADCCGRK"
     gene            complement(3209..4519)
                     /gene="bioA"
                     /locus_tag="VE030_13110"
     CDS             complement(3209..4519)
                     /gene="bioA"
                     /locus_tag="VE030_13110"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HZF24391.1"
                     /translation="MTSNDRLLSRSLLTVWHPCTQMKHHEHLPLLPIARGEGMWLVDF
                     EGKRYLDAISSWWVNLFGHANPRINAALTDQLGKLEHVMLAGFTHEPVVQLSERLSSL
                     TGLSHCFYGSDGASATEIALKMSFHAWRNGGQPRKTQFVSLHGSYHGETLGALSVTDV
                     PLFRDTYAALLRHSAHVPTPDWRLAQSGETHADVALRAARALEAYLAEHHEQTAALIV
                     EPLVQGASGMVMYHPRYLSEARALCDRYRVHLIADEIAVGFGRTGTFLACEQAGIRPD
                     FLCLSKGITGGYLPLSVVLTTEDIYSAFYHDDLARGFLHSHSYTGNALACRAALAVLD
                     IFEEDRVLEGNREKSAQLSRRCGKLAQHPGVRHFRNTGMIWAFDAASDDPAFARKFYQ
                     AALARGLLLRPLGNTVYFMPPYLIDEENMDFLVDGTGAILDSLL"
     gene            4780..5418
                     /locus_tag="VE030_13115"
     CDS             4780..5418
                     /locus_tag="VE030_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZF24392.1"
                     /translation="MNLLESISDRMAVTNRPLFAITVAAVPCPDTPVILTLHWHGFIE
                     EKIIEVEEAETVSYTPIPSSALQVNGRWDDLTILDRAAMEAGWELGAWDVARAERVGC
                     LRPGVESYEALECLQAFGAFPYGVNGHQVVVSDAPDAEDLLNLAARRGYLMWMFRPVH
                     GGIWAEVADDETLRPDGTRTPPCPIAPVPPAGSGSRKTVYRFGISGSPVRPN"
     gene            5646..>5779
                     /locus_tag="VE030_13120"
     CDS             5646..>5779
                     /locus_tag="VE030_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HZF24393.1"
                     /translation="MKIRPLHDRVIVKRLEEERKTASGIVIPDTAAEKPDQGEIIAVG
                     "
BASE COUNT         1074 a         1771 c         1815 g         1119 t
ORIGIN      
        1 acctggtacg cgaaaggaca gccccaggca tcgctgcaac tggtgtacat gaacgacagt
       61 accgtgactt tgccggtggt gaagcgggca agcggtatgg gctggtcgtc ggtatcgagc
      121 actgttccgt ccggtgcggt catgatgtgc tcgagtcggt aggagccggc gggcggcggc
      181 acgaattcga gttgcctggt cggctctcca tcgtcaccgg cagcaaacgc ggcggcggcc
      241 agaaacaggc cgccgcacaa tgcacaggga cgcagcatca gcgtgcggcc gcaatctcgc
      301 cgggcggctc cgcgtcccca aacgcggatt tcagcgactt cgagccaagc ttcatatgat
      361 gggcgcgtcc gagcttttcc ttggtgaagt ccacctcgaa agcgggcctt agttccttgc
      421 cgtcccagtt gaaggcgcgc aggaactgct cgttatccgt gcctttcttg tcccattttt
      481 ccagaagcga cgaggtcacg taaacgcgct tgccgtccca gctctgcgac accatgttga
      541 cctgcttgcc ggtgactttc tcgtaggtct gtttcggcgc ttccggattg gagaggtcga
      601 agtagcgggt cttgccgtcc atgaaagtgt tgacccacag acccttgccg tcgcggctga
      661 tgctgatgtc caccggcaac gggatctttg ccgggtcgcc gatggtggcg acatccttgg
      721 cttcccattc gccgttggcg ccctccctga tcagccagat cttcgaggtc agcgccgtgg
      781 cgacgaccgc ccagttctcg ccttccctga gcgaccagcg gatctccagc ggcgcacccg
      841 gcacgctcag gactttcttc ggcttcatcg ccttcagatc ccatagcacc atggtgttgc
      901 cgaacttctt catagcctcg gcatcggcaa cgacttctcc caggtcgcgc atgtaattgt
      961 tgaagccggt gaagctggag gtgagcagca ggttcttgcg cggattgatg ccgatatcgt
     1021 agccgtagcc gtcgccgcca ttatcggtgg ggaggtcgta cttgctgatc aggtcgccct
     1081 tgttgttgaa aagcgccagt ccggtgacgc cgcccttgtc ccgggtattg gaaagcgttc
     1141 ccaccagcat gcgcccaggc agcgcgtaga aagtatgcgg gccggcgtag ccggtccttt
     1201 ccggcagatc ggcgataact ttagtgagct tcggcttggc cggattggag ccgacgtcga
     1261 acacgtagat cctgctgttg gacagcccgc ctgcccacag gtggcggcgg tcgtcggtga
     1321 atcccatgtg gtgtgcttcg cctcgcgcgc ccaccgatat gctgtggatt gccttgccgt
     1381 actttttcga tgccggattg acgtcgatgg taaccagctt gtcggaaccg tcgccgagcc
     1441 cttgcacacc caaggtccag acgtacacgt aatcttcctg ccccttgatc aaattgccga
     1501 tgtaagggga attgcaggtt tcgtccgcgc ccgcataggg gccggcgccg agcaaagcgg
     1561 ccgcgagcaa agcggtggcc agcgatcttg gcacgcggga gagtctgagc atcatgtcct
     1621 ccgttggtct tggtactgtt tgggaagggt taccaatcta ggcattgatg aagggaaagc
     1681 caaatagaaa ttcgcgatta ggaaataacc gataccaccg attacttccg gccgcagcag
     1741 tccgcttggg atccctcctg cacccattcg aggagcgcgt ccacggcggc ctgcgcgttg
     1801 cgtgcgcctg gatgattgat cagaaacacg actaccagca tgcgtccgcg gctatccatg
     1861 acgtagccgg agatggcgcg tacgccgtcc aggtaaccgg tcttcacgtg tgcctggccg
     1921 gcaaccggcg agttgttgag acgccggcgc atggtgccgt ccaccgctgc cagcggcagc
     1981 gaggccacgt actcgggcat caacgggctg ttccaggcgg cgatgaggag ttcgccgagt
     2041 tggcgggcgg atatgcgggt ggtgcgtgaa agtcccgcac cattctcgat gcgcatttcg
     2101 gggaacgata atccccgtct tgccagccaa tcgctgaccg cgcgatcgga cttctccagc
     2161 gtggcaggct tgccgtcctg cgcgccgagc gtcaggaaga cattgcgggc catgacattg
     2221 ttgctgaact tgttgatgtc gcgaatgctt tccgccagcg cagccgattc ccagctcgac
     2281 aacaatcgcg cgttcgttgg cacctcggcc tcgcgcacgc gccccgacca gctgccgcca
     2341 gcctgcagcc agaattggcc gaaaacggca gccatgtact cgttcggggt gagcatgctg
     2401 aagctgcggg atttctcgcc gcagctggac ggaaacgtgc cgaagaaggt aagggtgttg
     2461 ccgtcgcgcg ccggcttgtc cggccagaca tcgcagaatc ccggtgcaag gtagagattg
     2521 ttgaccacca tgacctgggg caggaccggc tcggaataca ccgccaccgc gcgcttttcc
     2581 tcgtcgggaa cgaaacgaag ccggaatgac ttgaaattca gcagcagcgc atctgcgccg
     2641 acgttgtaag gccgcgtcgg ttcgttgtcg aactgcgccg gatcgtggtc gtcgatggcg
     2701 aagaaactgc gatccaggac cagatcgccg gtgatttcgc gcacgccgcg gttgcgcaag
     2761 tcgcgcacaa acagccagaa gttctcgagc gtcagtttcg gatcgccata gcccttcagt
     2821 accagatcgc cattcagtcg gtcgcccgta agggtcccgc gcagataagc ctccgttttc
     2881 caggtataag ccggtccgag aatctccagc gcggcaaacg tagtcaccag cttgatcact
     2941 gacgcgggat tcagcgatcg gtcgatgccg accgtcacta ccggcaggtt gttggccagg
     3001 tcatgaacgt aaattcccac gctgctctcc ggcacgccgg cttgtgccag cgcggctgcg
     3061 accgggccgg gcggctcgct catcggcgtg gtggccatgg cctgcgccgc cgtcgcgagc
     3121 agcagcaaga gcggcaaacg tccgagggcg ctcatcgcgt tgcgttcgcc gatacgcata
     3181 aaggcagccg cggaggagaa tcgtgtgctc agaggaggct gtccagaatt gcgccggtac
     3241 catcgaccag gaaatccatg ttctcctcgt cgatcaggta cggcggcatg aagtacacgg
     3301 tatttccgag cggccgtaac aacaatccgc gcgccagcgc ggcctgatag aatttgcgcg
     3361 cgaatgcggg gtcatcgctc gccgcatcga atgcccagat catcccggtg ttgcggaaat
     3421 gcctgactcc cggatgctgc gcaagcttgc cgcagcggcg cgaaagctgc gcagatttct
     3481 cgcggttgcc ttccagcact cgatcctcct cgaaaatgtc cagcaccgcc agcgcagcgc
     3541 ggcaggccag cgcgttgccg gtgtaggaat gcgagtgcaa gaagccgcgc gcgagatcgt
     3601 cgtggtagaa cgccgaatag atatcctcgg tagtcagcac caccgacagc ggcaggtagc
     3661 cgccggtgat gcccttggac agacagagga aatccggccg gatgcccgcc tgttcgcagg
     3721 ccaggaacgt gccggtgcgg ccgaatccca cggcgatttc gtcggcgatc aggtgcaccc
     3781 gatagcggtc gcagagcgcc cgggcttcgc tgaggtaacg cgggtggtac atcaccatgc
     3841 cgcttgcgcc ctgcaccagg ggctccacga tcagcgccgc agtctgctcg tggtgttcgg
     3901 ctaagtaagc ctccagcgcg cgagccgccc gtagcgccac gtctgcatgg gtttcgcccg
     3961 attgagccag gcgccagtcc ggcgtcggca catgcgcgct gtgtcgcagc agcgctgcgt
     4021 aggtgtcgcg gaacaacggc acgtcggtca ccgatagcgc gcccagcgtc tcaccgtgat
     4081 aactcccgtg caggctgacg aactgcgttt tccgcggctg tccgccgttg cgccacgcat
     4141 gaaagctcat tttcagcgcg atctcggtcg ccgaggcgcc gtcgctgccg tagaaacagt
     4201 gcgacagacc ggtcagcgac gacagccgtt cggaaagctg caccaccggc tcgtgggtga
     4261 atcccgcgag catcacgtgc tcgagtttgc cgagctggtc ggtcaacgcg gcgttgatcc
     4321 tgggattggc gtggccgaac aggttgaccc accaggaact gatcgcatcg aggtagcgct
     4381 tgccttcgaa gtcgaccagc cacatgccct cgccgcgggc gatcggcaac aacggcaggt
     4441 gctcgtggtg cttcatctgc gtgcacgggt gccagacggt gagcaggctg cgtgacagca
     4501 gtcggtcgtt gctagtcaat ttggactgtt cggatgtggg caatgcaact gcaagcgcgg
     4561 gaatgcgtca gaccaagtaa gacatctttt ccgcgttcaa tcaagttggc accgtaatac
     4621 gcgggtaatc gccagtgtgc gatgaagacg gcaacctcgc gccgggaccc gcgccggtac
     4681 tagcactcca ggcccgcggc tgctcgcgat actggcgtgt ttgatgcttg accctaggga
     4741 gaatgcttcg cactatgcgc ttgcgaaagg agggggttaa tgaatctgct ggaatcgatc
     4801 tccgaccgga tggccgtgac caaccggcct ctgtttgcga tcacggtggc agcggtacct
     4861 tgtcccgaca cgccggtgat tctgaccctg cactggcacg gcttcatcga agagaaaatc
     4921 atcgaggtgg aagaagccga aactgtgtcc tacaccccga tcccgagttc ggcgctgcag
     4981 gtcaacgggc gctgggacga tctgaccatt cttgatcgcg cggcgatgga agccggctgg
     5041 gagctcggcg catgggacgt ggcgcgtgcc gagcgcgtcg gttgcctgcg ccccggcgtc
     5101 gaatcgtacg aggcgctcga gtgcctgcag gctttcggcg cgttccctta cggcgttaac
     5161 gggcatcaag tcgtggtgtc cgacgcaccg gatgcggaag acctgctgaa tctcgctgcg
     5221 cggcgcggct acctgatgtg gatgttccgg cccgtgcatg gtggcatctg ggctgaagtg
     5281 gccgatgacg agaccctgcg gccggacggc acccgcactc cgccgtgccc aattgccccg
     5341 gtgcctccag ccggcagcgg gtcacgcaag actgtttacc gcttcggtat ctccgggagc
     5401 cccgtccgcc cgaactgacc aggcgaggcg cggcccaacg ccgccagtcc ggttgacccc
     5461 gcccgcattc ccaaactgcg ggactgtgag tgcttgctca cactatgcgt ggcgcgttta
     5521 ggcctgatta atcaaactaa gccttgaaat ccagtcgacg cacgcctatt attagcactc
     5581 gcaagcattg agtgctaacg aggctctcgc ctcgttattc gttcataaac aagaggagag
     5641 ttgagatgaa aatccgtcca ttgcacgacc gagtaatcgt caaacgcttg gaagaggagc
     5701 gcaagacggc ttccggcatc gtgatccccg acaccgctgc agagaagccc gatcagggcg
     5761 agatcattgc ggtgggcca
//
LOCUS       DASQOP010000335         4967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_977690, whole genome shotgun sequence.
ACCESSION   DASQOP010000335 DASQOP010000000
VERSION     DASQOP010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4967
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977690"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1023)
                     /gene="rluD"
                     /locus_tag="VE030_13125"
     CDS             complement(70..1023)
                     /gene="rluD"
                     /locus_tag="VE030_13125"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: protein motif:HMM:NF008385.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HZF24394.1"
                     /translation="MPNYIEDPWSGRHHHLAIPAQCAGMRLDQALVRLLPEYSRNRLQ
                     EWIRSGHVTMNGAPASAKAKVWGGETIVVAPQPAPGDTAHRPEDIPLQVVFEDDQILV
                     IDKPAGLVVHPGSGNWSGTLLNALLRHAPQLSGVPRAGIVHRLDKDTSGLLVVAKTIP
                     AQVDLVRQLQARSVTREYSAVVRGVVARDGVVEAPIGRHPVNRTRMAVVARGKAATTH
                     YAVLERGRSWSLLRCRLETGRTHQIRVHLHSIGHPLVGDPVYRLNNRSTTLPPEALDF
                     PRQALHAARLALVHPHTGETLTWQAPAPPDIVHLLWILRNA"
     gene            1043..1813
                     /locus_tag="VE030_13130"
     CDS             1043..1813
                     /locus_tag="VE030_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HZF24395.1"
                     /translation="MKRSVAILAMLLAAGCSSDPVDETKNWAPERIYSAAREELDGKN
                     YQKAIGYYEKLESRYPYGRYAQMAQIESAYAYWKDGEVAQALAACDRFIKLHPDHPNV
                     DYAYYLKGRINFNEDLGVMGYLAEKDMTERDPRAAREAFDAFRELVSRFPKSRYAADA
                     RARMNYLVNALAAAEVNVAQYYLRRGAYVAAVNRAQFALVNYPEAPAIERALKVLVKA
                     YDEMGLADLRDDARRTLDKNFPNSTAKLHESKSWWKLW"
     gene            complement(1937..3907)
                     /locus_tag="VE030_13135"
     CDS             complement(1937..3907)
                     /locus_tag="VE030_13135"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HZF24396.1"
                     /translation="MATTQNTPTVLWRPSSERVAKSNITRFIHMVNQRWNAGVRDSQQ
                     LYDWSVHEPEQFWTAVWDFCGVVAETRGERVLVDGDKMPGAKWFPDSRLNFAENLLRR
                     RDDDTAMVFWGEDKVRRRMSYAEVYDAVSRTVQALSASGVTEGDRVAAFMPNMPETII
                     FMLATTSLGAIWSSCSPDFGAQGVLDRFGQIGPKVLFAVEGYHYNGKTHDTLPRIAEI
                     ASNLPTLARTVIVSYTREKPALSGIRNAVHLADFVGPYRGREIAFRRLPFNHPLYILY
                     SSGTTGVPKCIVHGAGGTLLQHLKEHQLHADLKRGERLFYFTTCGWMMWNWLASGLAT
                     GVTLLLYDGSPFHPRPSVLFDFADEERMSVFGTSAKYIDALNKAGARPIDTHRLDSVE
                     TVTSTGSPLVPEGFDYVYRAIKKDVLLSSISGGTDIASCFVLGNPTAPVYRGEIQCRG
                     FGMNVQVFDDDGKPVIGAKGELVCTAPFPSMPIYFWNDPDGAKYRAGYFERFPDTWCH
                     GDYMELTPRGTAIIYGRSDATLNPGGVRIGTAEIYRQVEQLDEVVEGLVIGQDWDNDV
                     RVVLFVRLREGLALDDVLAKKIKDRIRLNTTPRHVPAKIVQVTDIPRTKSGKIVELAV
                     RNVVHGNPVKNLEALANPEALEQFRDRPELRG"
     gene            complement(4095..>4967)
                     /gene="lysS"
                     /locus_tag="VE030_13140"
     CDS             complement(4095..>4967)
                     /gene="lysS"
                     /locus_tag="VE030_13140"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008540856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HZF24397.1"
                     /translation="PGGAAARPFVTHHNALDMDLYLRIAPELYLKRLVVGGIEKVFEI
                     NRNFRNEGISTRHNPEFTMLEFYEAYQDYNYLMDLTEDLLRQVAQEVLGTTALSYQGE
                     TIDLGKRFDRLTMAEAIAKYNPKYPLVELGKPAYLRSALDAFKVEVFPTDGPGMLQLK
                     LFEQTTEDKLVQPTFIVAHPTDVSPLARRNDANPAVTDRFELFITGREIANGFSELND
                     PEDQAERFSEQARAKEAGDEEAMYYDADYVRALEYGLPPTAGEGIGIDRLVMLFTDSP
                     SIRDVILFPQMRPE"
BASE COUNT          931 a         1484 c         1598 g          954 t
ORIGIN      
        1 cgggtggtga tgagcgcgcg cacgtttgcc ggagccggcc agtcggggac gatccagtcg
       61 cgagacgggt caagcatttc tcagtatcca cagcaaatgc acgatatccg ggggtgccgg
      121 cgcctgccat gtcagcgttt cgccggtgtg cggatgaacg agcgcgagcc tggcggcgtg
      181 cagcgcctgg cggggaaaat ccagcgcctc cggaggcagg gtggtgcttc gattgttgag
      241 gcgatagacc ggatcgccga ccaacggatg tccgatcgag tgcaaatgca cgcgaatctg
      301 atgcgtgcga ccggtttcaa ggcgacagcg cagcagcgac caggaccgcc cacgctcgag
      361 cacggcgtag tgcgtggtgg ccgcctttcc ccttgcaacc accgccatcc tggtgcggtt
      421 caccgggtgg cggccgatcg gtgcttcgac gacgccgtcg cgcgccacca ccccgcgcac
      481 cacagcagag tattcgcggg taacggagcg tgcctgcagt tgccgcacca gatcgacctg
      541 tgcaggaatt gtcttggcca ccaccagcaa tccgctggtg tccttgtcga gtcggtgtac
      601 gattccggct ctgggcactc cggagagctg aggcgcgtgg cggagcaggg cgttcaacaa
      661 ggtgccgctc cagttgccgc tgccgggatg cacgaccaga ccggcgggct tgtcgatgac
      721 caggatctgg tcgtcttcga acacaacctg cagcgggatg tcctcggggc ggtgcgccgt
      781 gtcgccggga gcgggctgcg gcgcaacgac gatggtttcc ccgccccaca ccttggcttt
      841 ggcggaggcg ggcgccccgt tcatggtcac gtgcccgctg cgaatccatt cctggaggcg
      901 gttgcgcgag tactcgggca gcaaccgaac cagagcctga tcgaggcgca tgcctgcgca
      961 ttgcgccgga atggcgaggt ggtgatgccg gccagaccag ggatcttcta tataattcgg
     1021 caattctgtt gcggattccg tcatgaaacg tagtgtagcg attcttgcca tgttgctggc
     1081 cgccggatgt tcgtcggacc cggtcgacga aaccaagaac tgggccccgg aacggatata
     1141 cagcgccgcg cgggaggagc tcgacggcaa gaattatcag aaggcgatcg gctactacga
     1201 gaagctggaa tcgcgttatc cgtacgggcg ttacgcgcaa atggcgcaga tcgagagcgc
     1261 ctacgcgtac tggaaggacg gcgaagttgc ccaggcactg gcggcctgcg accgcttcat
     1321 caagttgcat cccgatcatc ccaacgtgga ttacgcctac tatctaaagg ggcggatcaa
     1381 cttcaacgag gacttgggcg tgatgggtta tctcgccgaa aaggacatga ccgagcgcga
     1441 cccgagagcg gcgagggaag cgttcgacgc gtttcgcgaa ctggtatcgc gctttccgaa
     1501 aagccggtac gcagccgatg cgcgggcacg catgaattac ctggtgaatg cgctggctgc
     1561 ggccgaggtg aacgtcgccc agtactacct gcggcgcggc gcctatgtcg cggcagtcaa
     1621 ccgcgcccag ttcgcgctgg tcaattatcc cgaggcgccg gcgatcgaac gcgccctcaa
     1681 ggtgctggtc aaggcctacg acgagatggg gcttgccgat ctgcgcgacg acgccaggcg
     1741 aacactggac aagaatttcc ccaattccac cgccaagctc catgagagca agtcatggtg
     1801 gaagctctgg taatccgaat cacgtcgagc agcgcgagat cgggctgaaa atccgcgatc
     1861 gcaacgacct cctcgcacgg gccggcggtt atcgcgcgaa gcgtttcact atcccctaat
     1921 ccctggcctc tcgttcctag cccctcagtt ccggcctgtc acggaactgc tccagcgctt
     1981 cgggatttgc gagcgcctcc aggttcttca ccgggttgcc gtgcacgaca ttgcgtaccg
     2041 cgagttcgac gatcttgccg ctcttggtac gcggaatgtc ggtgacctgg acgatcttcg
     2101 ccggtacgtg gcggggggtg gtgttgaggc gaatccggtc cttgattttc ttcgcgagca
     2161 cgtcgtcgag cgccagtcct tcgcgcaggc gcacgaacag caccacgcgg acgtcgttgt
     2221 cccaatcctg gccgatcacc aggccttcca ccacttcgtc gagctgctcg acctggcgat
     2281 agatctcggc ggtgccgatg cgtacgccgc cgggattgag tgtggcgtcc gaacgtccat
     2341 agatgatggc ggtgccgcgc ggggtcagtt ccatgtagtc gccatggcac caggtatccg
     2401 ggaaacgctc gaagtacccg gcccggtact tggcgccgtc gggatcgttc cagaaataaa
     2461 tcggcatcga agggaagggc gcggtgcaca ccagttcgcc tttcgcgccg atgaccggtt
     2521 tgccgtcgtc atcgaagact tggacgttca ttccgaagcc gcggcactgg atttcacccc
     2581 ggtacaccgg agccgtcggg ttgccgagca cgaagcagga agcgatgtcg gtgcctccgg
     2641 aaatcgagga tagcaggacg tctttcttga tcgcgcgata aacgtagtcg aacccctccg
     2701 gcactagtgg cgaaccggtc gaggtgacgg tttcgaccga gtccagccgg tgggtatcga
     2761 tcggcctggc gccggccttg ttgagcgcgt cgatgtattt ggcggaggtt ccgaaaacgc
     2821 tcatgcgctc ttcgtcggcg aagtcgaaca acacgctcgg tctgggatgg aaaggcgagc
     2881 cgtcgtacag aagcagcgtc acaccggtgg caaggccgga agcaagccag ttccacatca
     2941 tccagccgca ggtggtgaag tagaacaggc gctcgccgcg tttcagatcg gcgtgcagct
     3001 gatgctcctt gaggtgctgc aggagcgtgc cgccggcgcc gtgaacgatg catttgggca
     3061 cgccggtggt gcccgaggag tagagaatat agagcggatg gttgaacggc aggcgcctga
     3121 acgcgatttc gcgaccgcga tagggtccga cgaagtcggc gagatgcacc gcgttcctga
     3181 tgccggacag ggcgggtttc tcccgggtgt aggagacgat caccgtgcgc gccagcgtcg
     3241 gcagattgct cgcgatctcg gcaatgcgcg gcagcgtatc gtgggtcttg ccgttgtagt
     3301 ggtagccctc gactgcgaac agaaccttgg gtccgatctg gccgaaacgg tcgaggaccc
     3361 cctgcgcgcc aaagtccggc gaacacgacg accagatcgc accgaggctg gtggtggcga
     3421 gcatgaagat gatggtctcg ggcatgttcg gcatgaaggc cgcgacccgg tcgccttccg
     3481 ttactccgga ggccgacaac gcctgcactg tgcgcgaaac cgcgtcgtag acttcggcgt
     3541 agctcatgcg acggcgcacc ttgtcctcgc cccagaacac catcgcggtg tcgtcgtcgc
     3601 gccgccgcag caggttttcg gcaaagttca accgcgagtc cgggaaccac ttcgctcccg
     3661 gcatcttgtc gccatcgacc agaacccgct cgccacgagt ctctgccacc accccgcaga
     3721 aatcccagac ggcagtccag aactgctccg gttcatgcac cgaccagtcg tagagctgct
     3781 gcgaatccct gaccccggca ttccagcgct ggttgaccat gtggataaag cgcgtgatgt
     3841 tggacttggc gacgcgttcg ctcgacggcc gccagagtac ggtaggggtg ttttgggtcg
     3901 tggccatgga aatcaaacct tttcaccgca aaggcgcaaa gaacgcaaag aaaaacgaaa
     3961 attgtgaaat acaaagaaca tacaggcgtg aatgatgttt atccatactt ctgaatccgc
     4021 agcccgcgcc attctcaaat ctgcctgttc ttgttcctct ttgcgtcctt tgcttctttg
     4081 cggtgagagc ttttctattc agggcgcatc tgcgggaaca ggatcacatc ccggatcgag
     4141 ggactatcgg tgaaaagcat caccaggcga tcgatgccaa tgccttctcc cgccgtgggc
     4201 ggcagaccgt actcgagcgc gcgcacgtaa tcggcgtcgt agtacatcgc ctcctcgtcg
     4261 ccggcctcct tggctcgggc ttgttccgag aaccgttcgg cctgatcttc cgggtcgttc
     4321 agttccgaga atccgttcgc gatctcgcgg ccggtgatga acagttcgaa gcggtcggtg
     4381 actgccgggt tggcgtcgtt gcggcgggcc aggggcgaga cgtcggtcgg atgggcgacg
     4441 atgaaggtcg gctggacgag cttatcctcc gtagtctgct cgaacagctt gagttgcagc
     4501 attcccgggc cgtcggtagg gaagacctcg accttgaaag catcgagcgc cgagcgcagg
     4561 taagccggct taccaagttc caccagcggg tatttcgggt tgtacttggc gatcgcctcg
     4621 gccatggtca gccgatcgaa gcgtttgccc aggtctatgg tctcgccctg atagctgagc
     4681 gcggtagtgc cgagcacttc ctgtgcgact tgccgcagca ggtcctcggt gagatccatc
     4741 agatagttgt agtcctggta ggcctcgtag aactccagca tggtgaactc cgggttatgc
     4801 cgggtcgaga tgccttcgtt gcggaaattt cggttgatct cgaaaacctt ctcgattccg
     4861 cccaccacca gccgcttgag gtaaagctcg ggtgcgatgc gcaggtagag gtccatgtcg
     4921 agcgcgttgt gatgcgtgac gaacggccgc gccgccgcac cgccggg
//
LOCUS       DASQOP010000336         7374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_772371, whole genome shotgun sequence.
ACCESSION   DASQOP010000336 DASQOP010000000
VERSION     DASQOP010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7374
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772371"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VE030_13145"
     CDS             <1..337
                     /locus_tag="VE030_13145"
                     /EC_number="5.4.2.2"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011544765.1"
                     /note="catalyzes the interconversion of alpha-D-mannose
                     1-phosphate to alpha-D-mannose 6-phosphate and
                     alpha-D-glucose 1-phosphate to alpha-D-glucose
                     6-phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HZF24398.1"
                     /translation="LSGVPDIGAALDELPDSVNTPELQIKLKEGENYALIEKLQKTAR
                     FPEAREVITIDGLRVEYADGFGLARSSNTTPVVVLRFEAEDESALKRIQADFRRVLRE
                     AAPTAQLPF"
     gene            complement(351..2969)
                     /gene="alaS"
                     /locus_tag="VE030_13150"
     CDS             complement(351..2969)
                     /gene="alaS"
                     /locus_tag="VE030_13150"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011884423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HZF24399.1"
                     /translation="MTSNEIRSRFLDYFKRQGHTIVPSSPLVPGNDPTLLFTNSGMVQ
                     FKDVFLGKEKRPYVRAATSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     RDAIRFAWELLTREFGLDPARLWVTVYQTDDEAYDLWTKDIKVPKERIGRIGDTPGGS
                     KFQSDNFWQMGDTGPCGPCTEIFYDHGPGIPGGPPGTADADGDRYIEIWNLVFMQFNR
                     DESGTLHPLPKPSVDTGMGLERIAAVLQRVHSNYEIDLFRDLIAAAARVTGAKDLDSS
                     SLKVIADHIRACAFLIIDGVIPGNEGRGYVLRRIIRRAIRHGYKLGQTKPFFHKLVGD
                     LSRVMGEAYPELPAAQHRVADVLKQEEERFAETLENGMKVLESALHREDRMLDGETVF
                     QLYDTFGFPVDLTADIARERGVMIDHAGFEEAMEKQRERARAASKFTMQAGMDYSGQA
                     TEFHGYDTLRLDGKIVALYRGGAPVQRIEAGDAAVVVLDRTPFYAESGGQVGDQGEIA
                     SGNGSFTVDDTQKIQAEVFGHKGVLKAGRLAVGDAVTAQVNPVTRTRAAYNHSATHLM
                     HAALRKVLGLHVMQKGSLVDANRTRFDFSHHAPMTPEQIAEVEALVNREIRSNHAVET
                     RRMKYDEAIKAGAMALFGEKYGDEVRVLRMGDFSTELCGGTHVRRTGDIGFFKIVSES
                     GVAAGIRRVEAVTAEGALAWVKEQEARLGEAAQVLKAQPQEILQKLGQILDNVKSLEK
                     ELARLKSRLASLQGDDLAEQAVAIGKVRVLAAKLDGADVRTLREAMDKLKHKLKSAAI
                     VLGAVEGDKVTLIAGVTDDLTSKVKAGELVNYVAQQVGGKGGGRADMAQAGGTQPARL
                     PDALASVADWVKQRVA"
     gene            complement(3064..3513)
                     /gene="recX"
                     /locus_tag="VE030_13155"
     CDS             complement(3064..3513)
                     /gene="recX"
                     /locus_tag="VE030_13155"
                     /inference="COORDINATES: protein motif:HMM:NF001055.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HZF24400.1"
                     /translation="MATVPSLRARALKALGRREHSRQELQVKLQPFAQDSDELDALLD
                     ELENRGWLSEARFVEQLTTTRRRKFGAARIVHELREKGVSVGAISAAHAQLKAGEVEA
                     ARAVWKKKFGKLPRSLQERGKQARFLAGRGFSAEVVHQVLKDGNEQE"
     gene            complement(3523..4545)
                     /gene="recA"
                     /locus_tag="VE030_13160"
     CDS             complement(3523..4545)
                     /gene="recA"
                     /locus_tag="VE030_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HZF24401.1"
                     /translation="MDENRSKALAAALSQIEKQFGKGSIMRLGEKEVAKDLDVVSTGS
                     LGLDLALGIGGLPRGRVVEIYGPESSGKTTLTLSVIAQMQKMGGTAAFIDAEHALDPQ
                     YAQKLGCNVSELLISQPDNGEQALEITDMLVRSGSVDVVVVDSVAALTPKAEIEGEMG
                     EPQMGLQARLMSQALRKLTANIKRSNTLVIFINQIRMKIGVMFGNPETTTGGNALKFY
                     ASVRIDIRRTGSIKKGEEVIGNETRAKVVKNKVAPPFRQAEFDILYGEGISREGEIIE
                     LGVLYKLIEKSGAWYSYGKDRIGQGKDNTRDFLKSNPEVAKEIEARIRAHVAEKPLAQ
                     VAVAEE"
     gene            complement(4645..5139)
                     /locus_tag="VE030_13165"
     CDS             complement(4645..5139)
                     /locus_tag="VE030_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HZF24402.1"
                     /translation="MDAELYALAGKVGDALKARKLMLATAESCTGGWVGQAVTMVPGS
                     SHWFDRGFVTYSDDAKREMLGVRAQTLKKFGAVSEQTVREMIKGTLSHSKAHVALAVS
                     GIAGPDGGSPGKPVGTVCLAWGMKKGPPPHSVVRHYAGDRDAVRRQSVIAALEGVLAL
                     LEEP"
     gene            complement(5347..5859)
                     /locus_tag="VE030_13170"
     CDS             complement(5347..5859)
                     /locus_tag="VE030_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HZF24403.1"
                     /translation="MEASITSADAHGRPSLRFLVSHPAHFIAFGFGVGLSPVAPGTLG
                     TLLALPMYAWLAPRLDNQAFLLLLAGMFFVGAWACEISGRRLGVHDHGGMVWDEVTAF
                     LAVLFFTPATPLWQVSAFLLFRLFDIVKPMPIRYFDRNIRNGLGVMIDDMLAAFYTLL
                     CLAGWKFAFG"
     gene            complement(5834..6790)
                     /gene="thiL"
                     /locus_tag="VE030_13175"
     CDS             complement(5834..6790)
                     /gene="thiL"
                     /locus_tag="VE030_13175"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HZF24404.1"
                     /translation="MLSEFDLITRYFNRPTRHTVLGVGDDAALLALTPGHELAASTDT
                     MVEGVHFFPDVEPDSLGHKALAVNLSDLAAMGATPKWAMLALTIPKADETWLEDFARG
                     FFALAAEHDVDLIGGDTARGPRNICVQIMGEVPHGRALRRDGAKVGDDVWVSGHIGDA
                     AAAVAHLKGDLRLRGSLLSHCLARLNRPAPRVALGRLLVGVANSAIDISDGLIADLGH
                     ICERSAVGAAIEFAAVPCSAELMPLRGQALVIRAILTGGDDYELCFTAVAERRSEIEA
                     LSGQAGLALTRIGSIVAGEGVEVVDPAGKPMSVKDGGFDHFR"
     gene            6789..7058
                     /locus_tag="VE030_13180"
     CDS             6789..7058
                     /locus_tag="VE030_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24405.1"
                     /translation="MACPAPVSAVAPHRDDIVLILLLRQAIVRKLRTDIAGSAKLGDW
                     IEARVDIQKIGRTLAFANAYLYVDEARIARVSGVFVVQRPPREKP"
BASE COUNT         1258 a         2455 c         2208 g         1453 t
ORIGIN      
        1 cctgagcggc gtgcccgaca tcggcgcggc gctcgacgaa ctgcccgact cggtcaatac
       61 gccggagctg cagatcaagc tgaaggaagg cgagaactac gcgctcatcg aaaagttgca
      121 gaagaccgcg cgttttccgg aggcgcgcga ggtgatcacc atcgacgggc tgcgggtgga
      181 atacgcggac ggtttcggcc tggcgcgctc gtcgaacacc accccggtgg tggtactgcg
      241 cttcgaagcc gaggacgaat ccgcgttgaa gcgcatccag gcagacttcc ggcgcgtgct
      301 gcgcgaagcc gcgccgaccg cgcaactccc cttctaggtg agaattggtt ctacgccacc
      361 cgctgcttca cccaatcggc gacgctggcc agcgcatccg gaagtctcgc aggttgcgtt
      421 ccgcctgctt gcgccatgtc cgcccggccg ccgcctttgc cgccaacctg ctgagccaca
      481 tagttcacca gttcgcccgc cttgaccttg gaggtcagat cgtcggtcac gccggcaatc
      541 agagtgacct tgtcgccctc gactgcgccg agcacgatcg cggccgactt gagtttgtgc
      601 ttgagcttgt ccatggcttc gcgcagcgtc ctgacgtccg ccccatcgag cttcgccgcc
      661 agcacccgca ccttcccgat cgccaccgcc tgctccgcaa gatcgtcgcc ctgcaaactg
      721 gcgagcctgg atttgagccg cgccagttcc ttctccagcg attttacgtt gtcgagtatc
      781 tggccgagct tttgcaggat ctcctgcggc tgggctttca acacctgcgc tgcttcgccg
      841 agtctggctt cctgctcctt cacccaggcg agcgcgccct ccgcagtcac cgcttccacc
      901 cggcggatgc ccgcggccac cccggattcg gaaacgatct tgaagaaacc gatgtcgccg
      961 gtgcgccgca catgggtgcc gccgcacaat tcggtggaaa aatcgcccat gcgcagcacc
     1021 cgtacttcgt ctccgtattt ctccccgaac agtgccatcg ctccggcctt gatcgcctcg
     1081 tcgtacttca tgcgccgtgt ttccacagca tggttgctgc ggatctcgcg attcaccagt
     1141 gcctcgacct cggcgatctg ctcgggcgtc atcggcgcgt ggtgggaaaa gtcgaagcgg
     1201 gtgcggttgg catccaccaa tgagcctttc tgcatgacgt gcagaccgag taccttgcgc
     1261 aatgccgcgt gcatcaggtg ggtggcggaa tggttgtatg cggcacgcgt gcgcgtcacc
     1321 ggattcacct gcgcggtaac cgcatcgccg accgccaaac gtccggcctt gagcacgccc
     1381 ttgtggccga acacctccgc ctggatcttc tgcgtatcgt cgaccgtgaa gctgccgttg
     1441 ccggaagcga tctcgccctg gtcgccgacc tggccgccgg actcggcgta aaacggcgtg
     1501 cggtcgagca cgaccaccgc cgcgtcgccg gcctcgatcc tctgcaccgg cgcgccaccc
     1561 ctgtagagcg ccacgatctt gccgtccagg cgcagcgtgt cgtagccgtg aaactcggtg
     1621 gcctgtccgc tgtaatccat ccccgcctgc atggtgaact tggacgcggc gcgggcgcgc
     1681 tcgcgctgct tttccatcgc ctcttcgaag ccggcgtggt cgatcatcac gccgcgttcg
     1741 cgggcaatgt cggcggtgag gtcgaccggg aaaccgaagg tgtcgtaaag ctggaacacg
     1801 gtctccccgt ccagcatccg gtcctcgcgg tgcaacgcgc tctcgagcac cttcatgccg
     1861 ttttccagcg tctcggcgaa gcgctcctct tcctgcttga gcacgtcggc cacccggtgc
     1921 tgcgcggcgg gcaattccgg ataggcctcg cccatcaccc gcgacaggtc gccgaccagc
     1981 ttgtggaaga acggcttggt ctggccgagc ttgtagccgt gacggatggc acggcggatg
     2041 atccggcgca gcacgtagcc tcggccttcg ttgccgggaa tcaccccgtc gataatcagg
     2101 aacgcgcacg ctcggatgtg atcggcgatc accttgagtg agctcgaatc gagatccttt
     2161 gcgccggtga ccctggccgc ggcggcgatc aggtcccgga acaggtcgat ctcgtagttg
     2221 ctgtgcacgc gctgcagcac tgcggcgatg cgctccagcc ccattccggt gtccaccgag
     2281 ggtttgggca gcgggtgcag cgttcccgat tcgtcgcggt tgaactgcat gaacaccaga
     2341 ttccagatct cgatgtagcg gtcgccgtcg gcatccgcgg tgccgggcgg gcctccggga
     2401 atcccggggc cgtggtcgta gaagatttca gtacacgggc cgcagggacc ggtatcgccc
     2461 atctgccaga agttgtcgct ctggaacttg cttccaccgg gcgtgtcacc gatgcgaccg
     2521 atgcgctcct tcggcacctt gatgtccttg gtccagaggt cgtaagcctc gtcgtcagtc
     2581 tgatacaccg tgacccatag gcgggcggga tcgagcccga attccctggt cagcagttcc
     2641 caggcaaaac ggatggcgtc gcgcttgaaa tagtcgccga agctgaaatt gcccagcatt
     2701 tcgaagaagg tgtgatggcg cgcggtgtag ccgacattct ccagatcgtt gtgcttgccg
     2761 ccggcgcgaa cgcaacgctg cgaggtggcg gcgcggacgt agggccgctt ttccttgccg
     2821 aggaacacgt ccttgaactg caccatcccg gaattggtga acaacagcgt cggatcgttg
     2881 cccgggacca gcgggctgga agggacgatg gtgtgcccct gacgcttgaa gtagtcaagg
     2941 aaacgactgc gaatttcatt gcttgtcatt gactgaataa ccgctggatc tcgaaattca
     3001 gccggtgcga atccggccat tacttgtttg aattctattg cattctccgc ccccggggca
     3061 ccgtcattcc tgctcgttgc cgtctttgag cacctggtgc accacttcgg cggagaaacc
     3121 ccggccggcc aggaagcgcg cctgcttgcc gcgctcctgg agcgaccgcg gcaacttgcc
     3181 gaacttcttc ttccacaccg cccgcgccgc ttcgacttcg cccgccttta actgcgcgtg
     3241 cgctgcggag atcgcgccga ccgacacgcc cttttcgcgc agctcgtgga cgatgcgcgc
     3301 ggcgccgaac ttgcgccgcc gcgtggtcgt caactgctcg acgaaacgcg cctccgagag
     3361 ccagccgcgg ttttccagtt cgtcgagcaa tgcgtccagc tcgtccgaat cttgagcaaa
     3421 gggttggagc ttgacctgca gttcctgacg cgagtgttcg cgccgcccca gcgccttcag
     3481 tgcccgggcg cgcaggctgg ggacagtggc caagttggcg cgttactcct cggcgacggc
     3541 cacctgggcc agcggctttt cggcgacatg ggcgcggatc ctggcctcga tctccttcgc
     3601 cacttcggga ttgctcttga ggaagtcgcg ggtgttgtcc ttgccctgac cgatgcggtc
     3661 cttgccatag ctgtaccagg cgccggattt ctcgatcagc ttgtagagca cacccagctc
     3721 gatgatctcg ccttcgcgcg aaatgccttc gccataaagt atgtcgaact cggcctggcg
     3781 gaatggcggc gccaccttgt tcttgaccac tttggcgcgg gtctcgttgc cgatcacctc
     3841 ttcgcccttc ttgatcgagc cggtgcggcg gatgtcgatg cgcaccgagg catagaattt
     3901 gagcgcgttg ccgccggtgg tggtctccgg gttgccgaac atgacgccga tcttcatgcg
     3961 gatctggttg atgaaaatca ccagcgtgtt ggagcgtttg atgttggcgg tgagctttct
     4021 gagcgcttgc gacatcagcc gcgcctgcaa ccccatctgc ggttcgccca tctcgccttc
     4081 gatttcggcc ttcggcgtca gcgccgccac cgagtccacc actacgacat ccaccgaacc
     4141 ggaacgcacc agcatgtcgg tgatctcgag cgcctgttca ccgttgtcgg gctgcgaaat
     4201 cagcagttcg gaaacgttgc agccgagttt ctgcgcgtat tgcggatcga gtgcatgctc
     4261 ggcgtcgatg aatgcggcgg tgccgcccat cttttgcatc tgcgcgatca ccgatagcgt
     4321 cagcgtggtc ttgcccgacg attccgggcc gtagatttct accacgcgcc cgcgcggcag
     4381 tccgccaatg cccagcgcca ggtccaggcc cagcgagccg gtggagacca cgtccaggtc
     4441 cttggccact tccttctcgc caaggcgcat gatcgagccc ttgccgaact gtttttcgat
     4501 ctgcgacaag gcggcggcca gcgccttgct tctgttttcg tccatgcgtt tcacctcatt
     4561 ttttgaagcg gccattatgg cataaggcac cgcggcgtcc gaaggcggaa ttcttcctcg
     4621 ttttcagata cctgtgcctg gaagtcaggg ctcttcgagc aacgccagca ctccttccag
     4681 cgcagcgatg acggactgtc tgcgcacggc gtcgcggtct ccggcgtaat ggcgcacgac
     4741 gctgtgcggc ggcggcccct ttttcatgcc ccaggccagg cacaccgtcc cgaccggctt
     4801 gccgggcgag ccgccgtccg gaccggcgat gccgctgacc gccagcgcca cgtgggcctt
     4861 gctgtgcgag agcgtccctt tgatcatctc gcgcaccgtc tgctcggaaa ccgcgccgaa
     4921 cttcttcagt gtctgcgcgc gcacacccag catctcgcgc ttcgcgtcgt cggagtaagt
     4981 aacgaatccg cgatcgaacc agtgcgagct gcccggcacc atggtgacgg cctgcccgac
     5041 ccagcccccg gtgcacgatt ccgccgtggc cagcatcagc ttgcgcgcct tcagcgcatc
     5101 gccgactttt cccgccagtg cgtaaagctc agcgtccatc ccattcacct catgaaaagc
     5161 tattcaccgc ggaggacacg gatcaaagag cgcctctccc cggccctctc cagcttgtgg
     5221 agagggagcc aacgtcagcg agataattgt aaggttctcg ttttgcacac cctctccgcc
     5281 cgcttgcggg gggagaggga tggggagagg tgggtgtcct ccgtggttca agtacggtat
     5341 ttaagttcat ccgaacgcaa atttccaccc ggccaggcac agcagcgtat agaaggccgc
     5401 cagcatatcg tcgatcatca cacccagccc gttgcgaata ttgcggtcga agtagcgtat
     5461 cggcatcggt ttgacgatgt cgaacaaccg gaacagcaga aatgccgaga cctgccacag
     5521 tggtgtggcc ggcgtgaaga acagcaccgc caggaaagct gtgacttcgt cccacaccat
     5581 gccgccgtgg tcgtgcacgc ccagcctcct gccgctgatc tcgcacgccc atgccccgac
     5641 gaagaacatg ccagcgagca gcagcaggaa cgcctgattg tcgagacgcg gcgccagcca
     5701 ggcgtacatg ggcagcgcca gcaatgtgcc gagggtcccg ggcgcgaccg gggacaggcc
     5761 gacaccgaat ccgaatgcga tgaaatgcgc cgggtgcgaa accagaaatc gcaacgacgg
     5821 gcgcccgtga gcgtcagcgg aagtgatcga agcctccatc cttcacgctc atcggttttc
     5881 cggccgggtc cacgacttcg accccttcgc ctgccacgat gctgccgatg cgggtcaatg
     5941 ccagtcctgc ctgacccgac aacgcctcga tctcggatct gcgttccgca accgcggtga
     6001 agcacaactc gtaatcgtcg ccgccggtca ggatcgcccg tattaccagc gcctgtcccc
     6061 gcaacggcat cagctccgcc gagcagggta ccgcggcgaa ctcgatcgcc gcaccgaccg
     6121 cggagcgttc gcagatgtgc cccaggtcgg caatcagtcc gtcggaaatg tcgatcgcgc
     6181 tgttggccac gccgaccagc agccgtccca acgccacacg cggcgccggc cgattcagcc
     6241 gcgccaggca gtgcgacagt aacgagccgc gcagcctgag atcgcccttt aggtgggcaa
     6301 ccgcggccgc ggcatctccg atatgcccgg aaacccagac atcatcgccg actttggcgc
     6361 cgtcgcgccg caacgcccgg ccgtgcggaa cttcccccat gatttgcacg cagatgttgc
     6421 gcggcccgcg cgcggtatca ccgccgatca aatccacgtc gtgttccgcg gcaagcgcga
     6481 agaacccgcg cgcaaaatct tccagccagg tttcgtcggc cttcggtatc gtcagcgcca
     6541 gcatcgccca tttcggcgtc gcccccattg ccgccaggtc ggacagattg accgccagcg
     6601 ccttgtgccc gagcgagtcg ggctccacgt ccgggaagaa atgcacgcct tcgaccatcg
     6661 tgtcggtcga ggcggcgagt tcgtgcccgg gcgtaagcgc aagcaacgcg gcgtcgtccc
     6721 ccactcccag caccgtgtgc cgcgtaggac ggttgaaata cctggtgatg agatcgaact
     6781 cggacagcat ggcttgcccg gcacctgtgt cggctgtcgc gccgcatcgg gacgacatcg
     6841 ttctgatttt attgcttcgg caggcaattg tccgcaaact tcggaccgac attgccggca
     6901 gcgcaaaact cggcgactgg atcgaagcgc gggtggacat ccagaaaatc ggcaggacgc
     6961 tggccttcgc caacgcctat ctgtacgtcg atgaggcccg catcgcccgc gtttcaggcg
     7021 tcttcgttgt ccagcgaccg ccacgcgaaa aaccttgaac cacggagggc acggaggaca
     7081 cggaggaaac ccgaatgcat gtttgatatg aaaatctggc gaagttggtt tccaaccacc
     7141 gtacccggcc tttcatttcg atggggtttc ttttttgttc ttcctctgtg tcctccgtgt
     7201 cctccgtggt tcatgggtcc tgatcaactc ttcccgagtt tttgcaggaa ggcctggtac
     7261 tcgtcttcct tcatggtgaa gctgtgctcg ggcaatggga attgcccctt gtgcacgtct
     7321 tcctgaaagt ccgcgagcgc cttgccgatg atgtcccagc agttggcgta gcgc
//
LOCUS       DASQOP010000337         5758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2392245, whole genome shotgun sequence.
ACCESSION   DASQOP010000337 DASQOP010000000
VERSION     DASQOP010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5758
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2392245"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VE030_13185"
     CDS             complement(<1..213)
                     /locus_tag="VE030_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZF24406.1"
                     /translation="MDTVTPAFTGHSKDSRHPGAARVGIGGPVGSGKTRLLEQLIPRF
                     ISRGTNLAVITNDLATKEDAERVRRSG"
     gene            complement(357..1508)
                     /locus_tag="VE030_13190"
     CDS             complement(357..1508)
                     /locus_tag="VE030_13190"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HZF24407.1"
                     /translation="MDERMNFMAEIERGVRSVSEICRLFGVSRKTGYKWIGRFEQDGA
                     EGLKARSRAALVHPNAVDTQLVGMLLATRRRHDDWGPQKLLAYLGARHPQRHWPAIST
                     VAQLLNRHGLIKARKPRSRSAPYSAPFVRANAPNALWSADFKGQFRTGDGHYCYPLTI
                     SDGFSRYLISCRGLLHPSQALTQPWFERAFRDFGLPAVIRTDNGAPFASTGVGGLSKL
                     SIWWLKLGIVPERIRLGHPEQNGRHERMHRTLKRRCAVKTHLHAQQRAFNRFRVEYNQ
                     ERPHRALNDRTPAMLYQPSNRELPGKVPRFGYPNDCAVRRVHPNGEITWHTQPYYLGQ
                     VLAGEDVGLYPIADGRWRILVGTLAVAELDLRAQTIVSIDVHIDVPTLH"
     gene            complement(1659..2357)
                     /locus_tag="VE030_13195"
     CDS             complement(1659..2357)
                     /locus_tag="VE030_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZF24408.1"
                     /translation="MALLKTTKLESRYGDFQALFGIDFEIHPGEIVAMIGANGAGKST
                     FLKTVCGLLKADRDAVEFDGAPIGGVPPGSIVRLGVAMVPEGRRLFPSLSVEENLLMG
                     AYAKRPGPWNLDAIYGLFPLLKGKRRDPATALSGGQQQIVAIGRALMSNPRLLICDEI
                     SLGLAPIVVKEIYDALPNICAGGLTAIIVEQDVGVAQQVSSRLYCLQEGRVTLTGASK
                     ELSRAQISQAYFGI"
     gene            complement(2540..3262)
                     /locus_tag="VE030_13200"
     CDS             complement(2540..3262)
                     /locus_tag="VE030_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZF24409.1"
                     /translation="MAVILALENISKSFGALKVADRITLSVATGETLGILGPNGAGKT
                     TLFNLISGDLRADSGRVVFGAEDVTLHPPHRRCRLGIGRSYQIPQPFGAMTVFENLVA
                     AASFGAGRAEKDTYGHAVEILRETGLLSKANRLAASLTLLDRKRLELARALATKPKVL
                     LLDEIAGGLTEHEAGELVEEIRRIKSQGVTMIWIEHVVHALLAVADRLFVINFGQKLA
                     EGAPGAVMNDPEVKRVYMGLEA"
     gene            complement(3351..4631)
                     /locus_tag="VE030_13205"
     CDS             complement(3351..4631)
                     /locus_tag="VE030_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZF24410.1"
                     /translation="MQNNRFQRRRALMQAGGAVAGAAALGMPSAVFAQAKTIKLGFVS
                     PQTGPLAPFGEADKFIIEQMNKLFAGGVDLGGKKVAVQIIQKDSQSSPNRAGEVANDL
                     ILKDKVDLMLVSSTPETTNPVSDACELNEIPCISTVAPWQPWFFGRKGDPATGFNWTY
                     HFFWGLEDVIANFLAGWKSVPTDKKVGGLFPNDGDGNAWGDKERGFPGPLAKEGFTLL
                     DPGRFQNMNNDFSAQIAAFKKEGVEIVTGVVIPPDAKTFLTQARQQGFRPKVVTLGKA
                     LLFPAAIEALGDLGDGLSTEVWWSPSHPFTSSLTKQSAKQLADFYESTSKKQWTQPIG
                     FAHALFEVAVDALKRTKDLGSHASIRDAVAATSLQTVVGDVKWGGGGPFKNVSKTPLV
                     LGQWVKGKKYKYELVIVSNPQFPAIPAPGKLKLM"
     gene            complement(4680..5753)
                     /locus_tag="VE030_13210"
     CDS             complement(4680..5753)
                     /locus_tag="VE030_13210"
                     /EC_number="1.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008346113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018506 - maleylacetate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetate reductase"
                     /protein_id="HZF24411.1"
                     /translation="MRAFVYNGQPSRVVFGRGALEKLGAEIERLGAKRALVLSTPEQR
                     ASAEDVSRRLGARSAGVYDKAVMHVPIETAEAARAVAKELNADCCVAVGGGSTIGLGK
                     AIALTSELPILAIPTTFAGSEMTPIYGITAQGQKKTGRDARVLPRTVIYDPQLTMTLP
                     ARIAGPSGMNAIAHCVEALYAEDANPITSLMAEEGIRALARSLPKVVSDPADLEARAE
                     ALYGAWLAGACLGNVGMAIHHKLCHTLGGSYNLPHAETHTVILAHAVHYNRDAAKDAM
                     ARISRALGGNGAANGDPAGGLHDLAREIGAPLSLKDIGMKADALDDAARLATQNPYYN
                     PRPIEYAAIRQLLQNAWEGRRPG"
BASE COUNT          987 a         1859 c         1765 g         1147 t
ORIGIN      
        1 cccgctgcgg cggacgcgct ccgcatcctc tttggtcgcg aggtcgttgg tgatgacggc
       61 gaggttggtg ccgcgggaaa tgaagcgcgg gatcaactgc tccaggagcc gcgtcttacc
      121 ggatccgacc ggaccgccga ttccgacgcg ggcggcgccc gggtggcgcg agtccttgga
      181 gtgtccggtg aaggctggag tcacggtatc catcgcattc cttttgtacc tactagcggt
      241 cgcgccacca ggaacgaggg gtgagggggc aaggcatttc cagcaattcc ctcatccctc
      301 atcctgtgca actcggtaca tgggtaacac tttattccag gcacataggt aacacttcaa
      361 tgcagcgttg gcacgtcaat gtgcacgtca atagagacga ttgtctgcgc tctgaggtcg
      421 agttccgcca cggccagcgt gccaacgaga atgcgccagc gtccatcggc aattgggtaa
      481 aggcccacat cttctccggc gaggacttgt cccagatagt agggctgcgt gtgccaggtg
      541 atttcaccgt tgggatgcac acgacgcacc gcgcagtcgt tcggataccc aaagcgtggc
      601 actttgcctg gcagttctcg gttagaaggc tgatacagca tcgccggggt gcgatcattc
      661 aatgcccgat gggggcgctc ctgattgtat tcgacacgaa agcggttgaa ggcgcgctgc
      721 tgggcgtgca gatgcgtctt gaccgcgcag cgacgcttga gcgtgcggtg catccgttcg
      781 tggcggccgt tttgctccgg atggcccagt cgaatgcgct ccggtacgat ccccagcttc
      841 agccaccaga tcgaaagctt ggacaatccg cccacgccgg tagaggcaaa gggcgcaccg
      901 ttgtcggtgc ggatgaccgc aggcagtccg aagtcacgga aggctcgctc aaaccagggc
      961 tgggtcagcg cctggctcgg atgcaacagt ccgcggcagc tgatcagata gcgactgaag
     1021 ccatcggaga tcgtcagggg atagcagtaa tgtccatccc cggtacgaaa ctgtcccttg
     1081 aagtccgcac tccacagtgc attgggggcg ttcgcccgta caaagggcgc actgtagggg
     1141 gcgctgcggc ttcgtggctt gcgcgccttg atcaaaccat gtcggttgag caactgcgcc
     1201 acggtggaga tggccggcca gtgccgttgc ggatggcgtg cgcccagata ggcaagcagt
     1261 ttctggggac cccaatcgtc gtggcgccgg cgtgttgcca gcagcatccc caccaattgc
     1321 gtatcgaccg cgttggggtg caccagcgca gcacgcgagc gggccttcag gccttcggcg
     1381 ccgtcttgct caaagcggcc aatccacttg tagcccgtct tgcggctcac gccaaacagc
     1441 cggcagatct cactcacgct gcggacacct cgttcgatct ctgccataaa attcattcgt
     1501 tcgtccatgg gacagctcgc tctccaggtc atgggcaggc cctccttgcc catcagtttt
     1561 tacctgttac ccatgcccct agaaaactgt tacctatgta ccaggattaa acctccctca
     1621 tccctcgtcc ctcgcccctc gtccctcgtc cctgtgtctc atattccgaa atacgcctgc
     1681 gaaatctgtg cacggctcag ttccttcgac gcgccggtca acgtcacccg gccttcctgc
     1741 agacagtaaa gccggctcga aacctgctgc gcgacgccga cgtcctgctc gacgatgatc
     1801 gccgtcagtc cgcctgcgca gatgttcggc agcgcgtcgt agatctcttt caccacgatc
     1861 ggcgccaggc ccagagagat ctcgtcgcag atcagcagcc tcggattgct catcagcgcg
     1921 cggccgatcg ccacgatctg ctgctggccg ccggacagcg cggtggcggg atcgcggcgc
     1981 ttgcccttca gcagcgggaa aaggccgtag atcgcgtcga gattccacgg gccgggccgc
     2041 ttcgcatagg cgcccatcag caggttctct tccaccgaca ggcttggaaa cagccggcgc
     2101 ccctccggca ccatcgcgac gcccagccgc acgatgctcc ccggaggaac cccgccgatt
     2161 ggcgcgccgt cgaactccac ggcatcgcga tcggccttga gcagaccgca cacggtcttc
     2221 aggaacgtgg acttgcctgc gccgttggcg ccgatcatcg cgacgatctc gcccggatgg
     2281 atttcgaagt cgatcccgaa cagcgcctgg aagtcgccgt aacgggattc aagtttcgtc
     2341 gtctttagaa gtgccatgcg actagggatt caccacagag acacagaggc acggagaaag
     2401 tcgatgcttt caatcaacat aagaaaacga gggaccgggt aggaacgcat gcttcgagcg
     2461 ggagaacaaa tggcaggcgt ttccatatcc ttcatttggt tcctctccgt gcctctgtgt
     2521 ctctgtggtg aaatttcttt cacgcttcca ggcccatgta aacgcgcttc acttccggat
     2581 cgttcatcac ggccccgggt gcgccctcgg caagcttctg cccgaagttg atcacgaaca
     2641 ggcggtcggc gaccgccagc agcgcgtgca ccacgtgctc gatccagatc atggtgaccc
     2701 cctgtgactt gatgcgcctg atctcctcga ccagttcccc ggcttcgtgc tcggtcaagc
     2761 cgccggcgat ttcgtccagc agcaatactt tcggttttgt ggccagcgcg cgcgccagct
     2821 ccagccgttt gcggtcgaga agcgtcaggc tggcggcgag ccggttcgcc ttggaaagca
     2881 atcctgtctc gcgcagaatt tccaccgcgt gcccgtaagt gtccttttcc gcccgtccgg
     2941 cgccgaagga tgccgccgcg accaggttct cgaacaccgt catcgcaccg aatggttgcg
     3001 ggatctgata ggagcgaccg atgccgagac ggcagcggcg gtgcggcgga tgcagcgtca
     3061 cgtcttcggc gccgaacacg acccggccgg aatcggcgcg aaggtcgccg ctgatcaggt
     3121 tgaacagcgt ggtcttgccg gcgccgttgg gtcccagaat gcccagcgtc tcgcccgtag
     3181 cgaccgacag cgtgatgcgg tcggccacct tgagcgcgcc gaagctcttg ctgatgtttt
     3241 ccagcgcaag aatgacggcc atttttttag ggatatcccg ttcttgctcc agacctccct
     3301 ctccccttgc gggagagggc ggggagaggg gagaggggag aggggtccgt ttacatcaac
     3361 ttcagtttgc cgggtgcggg aatcgcggga aactgcgggt tgctgacaat gaccagttcg
     3421 tatttgtact tcttgccctt gacccattgc ccgagcacca gcggggtctt gctgacgttc
     3481 ttgaacggac cgccgccgcc ccacttcacg tcacccacca cggtctggag gctggtggcg
     3541 gcgacggcgt cgcggatcga cgcgtggctg ccgaggtcct tggtgcgttt cagcgcatcc
     3601 accgcgactt cgaacagtgc gtgggcgaag ccgatcggct gggtccactg tttcttggat
     3661 gtggactcgt agaaatcggc gagttgcttc gcgctctgtt tggtcagtga cgaagtgaac
     3721 ggatgcgacg gcgaccacca tacttcggtc gacaggccgt cgccgagatc accaagcgct
     3781 tcaatggcgg cggggaacag cagcgccttg cccagcgtca ccaccttcgg cctgaagccc
     3841 tgctgcctgg cctgcgtcag gaaggtcttc gcgtccggcg ggatcactac gccggtgacg
     3901 atctcgacgc cctccttctt gaaggcggcg atctgcgccg agaaatcgtt gttcatgttc
     3961 tggaagcggc ccggatcgag cagcgtgaaa ccttccttcg ccagcggtcc cgggaatccc
     4021 ctttccttgt cgccccaggc attgccgtca ccgtcgttcg ggaacaggcc gccgactttc
     4081 ttgtcggtcg gcaccgactt ccagcccgcc aggaaattgg caatcacgtc ctcgagtccc
     4141 cagaagaagt gataggtcca gttgaacccc gtggccggat cgcctttgcg gccgaagaac
     4201 cacggctgcc acggcgccac ggtggaaatg caggggattt cgttcagctc gcaggcatcc
     4261 gataccggat tggtggtctc cggcgtcgac gacaccagca tcaggtcgac cttgtctttc
     4321 agtatgaggt cgttcgcgac ttcgccagca cggttcgggc tggattggct gtccttctgg
     4381 atgatctgca ccgcgacctt tttgccgccg agatcgacgc cgccggcgaa caacttgttc
     4441 atctgctcga tgatgaattt gtccgcctcc ccgaatggcg cgagcgggcc cgtctgcggc
     4501 gacacgaagc ccagtttgat ggttttcgcc tgcgcgaaca cggccgacgg catgcccagc
     4561 gccgctgcac cggctactgc gccgccggcc tgcatcagcg cgcgacggcg ctggaatcga
     4621 ttgttctgca tgactgctcc tcctctggtt atgttagtga tggtgtgaag ggtagtggat
     4681 cagcccggcc ttcgaccttc ccaggcgttc tgcaaaagtt ggcggatcgc cgcgtactcg
     4741 atcggtctcg ggttgtagta cgggttctgg gtggcgagcc gcgcggcgtc atcgagcgcg
     4801 tcggctttca tgccgatatc cttcagcgac agcggcgcac cgatctcgcg cgccaggtcg
     4861 tgaagtccgc cggcgggatc gccattcgcg gctccattcc cgccgagcgc ccgcgaaatc
     4921 cgcgccatcg cgtcctttgc ggcgtcacgg ttgtagtgaa ccgcatgcgc caggatgacc
     4981 gtgtgcgtct cggcgtgcgg caggttgtag ctgccgccca gcgtgtgaca gagcttgtgg
     5041 tggatcgcca tgccgacgtt tcccaggcaa gcgcccgcca gccaggcacc gtacagcgct
     5101 tccgcgcgcg cctcgagatc ggccggatcg ctcaccactt tcggcaggct gcgcgccagc
     5161 gcgcggatgc cttcctccgc catcagcgaa gtgatcgggt tggcgtcctc ggcgtagagc
     5221 gcctcgacgc aatgcgcgat cgcgttcatc ccgctcggcc ctgcgatcct ggccggcaat
     5281 gtcatggtca gttgcggatc gtagatcacc gtcctgggca gtacgcgggc atcgcgaccg
     5341 gtcttcttct gcccctgcgc ggtgatgccg tagatcggcg tcatttccga accggcgaag
     5401 gtcgtcggga tcgccaggat cggcagttct gaggtcagcg caatcgcctt gcccaggccg
     5461 atggtcgatc cgcctcccac cgccacgcag cagtcggcat tcagttcctt cgccaccgcg
     5521 cgcgcggctt cggcggtttc gatcgggacg tgcatcaccg ccttgtcgta cacgccggcc
     5581 gaacgcgcac ccaggcgtct tgatacatct tccgccgaag cgcgctgttc cggcgtggac
     5641 aacaccaggg cgcgcttcgc gcccaggcgc tcgatttccg cgcccagctt ctccagcgca
     5701 ccgcgcccga acaccacgcg cgagggctgg ccgttgtaga cgaacgccct cacgcgta
//
LOCUS       DASQOP010000338         2637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_174775, whole genome shotgun sequence.
ACCESSION   DASQOP010000338 DASQOP010000000
VERSION     DASQOP010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174775"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VE030_13215"
     CDS             <1..456
                     /locus_tag="VE030_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HZF24412.1"
                     /translation="DLARFEWTLGLAFDAADAAALRFEDVAAVPPEAWAGLRLTPHPA
                     LHVVDLRTNAVAIWNELKNADRGIAPECLPEPLRWAIWRKQLSPYFRSLEADEAWALD
                     ATRNRLSFGEICAGLTRWMPEEQAAPRAAALLRGWVDEGWIAALETVPP"
     gene            758..>2637
                     /locus_tag="VE030_13220"
     CDS             758..>2637
                     /locus_tag="VE030_13220"
                     /inference="COORDINATES: protein motif:HMM:NF027637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HZF24413.1"
                     /translation="MFRLFVLVTVCLAALLCRSAFGMTSAMVASCPAGMDQKVETLTG
                     MFNVHVICDHVLFEIPKPMLNRDMLVNTEFAALSTGSDFVAPGSVVDNRVVRWVRRGN
                     KIHLEDVRYEIWAPNMPNLQRGVESASLRTVIRAFDIITEGPDGAPVIDITGLFVTEV
                     PEGFALEFMQHFNMDRVDPKRSFIENVKAFPHNVDIRFYQTWVPDRSRLRKSVESDQE
                     RIAPGLGFIFHTSLLLLPEQPMRGRYFDERIGYFATPFDDYGTEEHGRVRRAFIQRYR
                     LEKKDPRAEVSEPVKPITFYLSREVPDKWRPFIKRGIEDWQEVFEKTGFRNAIVARDA
                     PSEQENPNWDPEDVRYNVIRWTPSGRQNAMGPAVVDPRSGEVISSHAIFWHDVLHLAE
                     NWFFTQVSPLNPSAQKLPLPDDVIGDMLRYIVAHEIGHALGLRHNFKGHSGYSVAQLR
                     NREWTEKWGTSASIMDYARLNYVAQPGDGAHLLPKFGHYDYFAIEWGYRQYSADMTFD
                     DEWPMLDQLAAKQVDDPMLRFGGEDEAAALDPKVNTNVVGGDPVEAADMGLRNIDRVM
                     PLLIPGTTHKGGDYGRLREMYATLIMQRHRELAAVAKVVGGVEETRYQAGRGSAPFKP
                     TPP"
BASE COUNT          487 a          819 c          834 g          497 t
ORIGIN      
        1 gacctggcgc ggttcgaatg gacgctggga ctggctttcg atgcggcgga tgcggccgcg
       61 ctgcgcttcg aggacgttgc cgcggtgccg cccgaagcct gggccgggct gcgactcacg
      121 ccgcatccgg cgcttcacgt cgtcgatctg cgcaccaatg ccgtcgcaat ctggaacgaa
      181 ctcaagaatg cggatcgcgg catcgcgccg gagtgcctgc ccgagccgtt gcggtgggcc
      241 atctggcgca agcaactctc gccatacttc cgttcgctcg aagccgacga agcctgggca
      301 ctggatgcca cgcggaatcg cctcagtttc ggcgaaatct gcgccgggtt gacgcggtgg
      361 atgcctgaag agcaggccgc gccgcgtgca gccgcgctgt tgcgcggctg ggtggacgaa
      421 ggctggatcg ctgctctcga aaccgttccc ccataaaaac gctcaccacg gaggacacgg
      481 aggacgcgga ggaaaacaaa aacgaggaaa aataaattgg ggtaggcgat gtgactgttc
      541 gtgatcatca gccgccaatt tcaactctgt gggagcatgc gccaaataga gctttttaca
      601 ctcgtttgct ttttgctctt gtcttcctcc gcgtcctccg tgtcctccgt ggtgagcgct
      661 cgtctttgaa tttggcctcc gtggtgaatg cttattcggt actgcactac aatcggcatg
      721 actattcgtt tcagtgcgcc cgggaggtga cgtggccatg ttcagactat tcgttctcgt
      781 aaccgtttgc cttgcggcgc tgctttgccg atcggcgttc ggcatgacct ctgccatggt
      841 tgcgtcctgc cctgccggga tggaccagaa ggttgaaacg ctcaccggaa tgttcaacgt
      901 gcacgtgatc tgcgaccacg tgctgttcga gataccgaag ccgatgctga accgggacat
      961 gctggtgaac accgagttcg ctgcgctctc caccgggagc gacttcgtcg ccccgggctc
     1021 cgtcgtggac aaccgcgtgg tgcgctgggt ccggcgcggc aacaagatcc atctcgagga
     1081 cgtgcgttac gagatctggg cgccgaacat gcccaacctg cagcgcggcg tggaatcggc
     1141 gtcgctgcgc accgtcatcc gggcattcga catcatcacc gaggggccgg acggcgcccc
     1201 ggtcatcgac atcaccggat tgttcgtcac cgaggtgccg gaaggcttcg cgctggaatt
     1261 catgcagcac ttcaacatgg atcgcgtgga cccgaaacgc tccttcattg aaaacgtcaa
     1321 ggcgttcccg cacaacgtcg acatccgctt ctaccagacc tgggtccccg atcgcagccg
     1381 gttgcgcaag tcggtggagt ccgatcagga gcgcatagcg ccggggctgg gcttcatctt
     1441 ccacaccagc cttctgcttc tgcccgagca gccgatgcgc ggacgctatt tcgacgaacg
     1501 gatcggctat ttcgccacgc cgttcgacga ttacggcacc gaggagcatg gccgggtgcg
     1561 gcgtgccttc atccagcgct accgcctgga gaaaaaggac ccgcgggccg aggtgtccga
     1621 accggtcaag ccgatcacgt tttatctgag ccgcgaagtt cccgacaaat ggcgcccgtt
     1681 catcaagcgc gggatcgagg actggcagga ggtattcgag aaaaccggtt tccgcaatgc
     1741 catcgtcgcc cgcgacgcgc cgtcggaaca agagaacccg aactgggatc ccgaggacgt
     1801 gcgctacaac gtgatccgct ggacgcccag tgggcgccag aacgcaatgg ggccggcggt
     1861 ggtcgacccg cgcagcggcg aggtgatttc ttcgcacgcg atcttctggc acgacgtgct
     1921 gcacctggcc gagaactggt tcttcaccca ggtttcgccg ctgaacccga gcgcgcagaa
     1981 gctgccgctg ccggacgacg tcatcggcga catgctgcgc tacatcgtcg cgcacgaaat
     2041 cggccacgcg ctggggctgc gccacaattt caaggggcat tccggctatt cggtggcgca
     2101 gttgcgcaac cgcgagtgga ccgagaaatg gggcacttcc gcttcgatca tggattatgc
     2161 gcggctgaat tatgtcgccc agcccggtga tggggcccac ctgctgccga agttcggcca
     2221 ttacgactac ttcgcgatcg aatggggcta ccggcagtat tcagccgaca tgactttcga
     2281 cgatgagtgg ccgatgctgg atcagctcgc cgccaagcag gtcgacgacc cgatgctgcg
     2341 cttcggcggt gaggacgagg ccgccgctct cgatcccaag gtcaacacca acgtggtcgg
     2401 cggggacccg gtcgaggccg ccgatatggg attgcgcaat atcgaccggg tgatgccctt
     2461 gctgatcccg ggcacaaccc acaagggcgg cgattacggc cgcttgcggg aaatgtacgc
     2521 gaccttgatc atgcagcgtc atcgcgaact tgccgcggtg gccaaggtcg tcggcggcgt
     2581 cgaagaaacg cgttaccagg ccgggcgcgg gtcggcaccg ttcaagccga cgccgcc
//
LOCUS       DASQOP010000339         5296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_775871, whole genome shotgun sequence.
ACCESSION   DASQOP010000339 DASQOP010000000
VERSION     DASQOP010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775871"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /gene="sucC"
                     /locus_tag="VE030_13225"
     CDS             <1..602
                     /gene="sucC"
                     /locus_tag="VE030_13225"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403972.1"
                     /note="catalyzes the interconversion of succinyl-CoA and
                     succinate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HZF24414.1"
                     /translation="LDATMVEINPLVVTKDNRVLALDAKMSFDDNAMFRRPRVTEMRD
                     SAEEDPREAQAAEFGLNYVGLSGDIGCIINGAGLAMATMDMIKHAGGEPANFLDVGGG
                     ASPERVAAAFNLVLSDQNVKAILVNIFAGINRCDWVAQGVVQAAKSLRVPLVVRLAGT
                     NVEEGRRIVRDCGLPIITADTLAEAAHKVVAASRGGDVK"
     gene            599..1489
                     /gene="sucD"
                     /locus_tag="VE030_13230"
     CDS             599..1489
                     /gene="sucD"
                     /locus_tag="VE030_13230"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HZF24415.1"
                     /translation="MSILIDATTPVILQGFTGDKATFHAKEMIEYGTKVVGGVTPGKG
                     GQTHLDRPVFNTVKDAVKATGAEASLAFVPPPYAADALMEAADAGLRLVCIITDGIPA
                     QDMMRVKRYLRRYPKPKRTTVVGPNCAGIISAGKAMLGIMPSNIYIPGNVGVVSRSGT
                     LGYEAGAQMKALGIGITTSVGIGGDPINGSSFLDHLILFEEDPETKAVIMIGEIGGPQ
                     ESEAAKWVKENMTKPVVGYVAGLTAPKGRRMGHAGAIISAEGDSASEKAEIMKSYGLF
                     VAPSAAELGSTVALALSRAG"
     gene            1556..4288
                     /locus_tag="VE030_13235"
     CDS             1556..4288
                     /locus_tag="VE030_13235"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HZF24416.1"
                     /translation="MNDPAGPADPAAPSREPPGNFSRAALLFDLLVGVVQRYEPPVAA
                     VLREEQTTEGMSAVLLARTLQAQGIWFQLLAIAEQNRDMRNRREVERERGYEQLRGTF
                     AAVFASAAAEGITGAEVHQALSGLRVRPVITAHPTEAKRVTVLERHRRIYLRLLDLES
                     PRWTERERQELIHATGDEIELLWLTGELKLSKPTIAQEVAWGLYFFNENLFDVVPQML
                     AKIESAFANQFPGEPIDVPLFFQFGSWIGGDRDGNPYVTSEVTRRTLWDTRLASLRRY
                     RIRLLDLARNLSIEDHALALPDSFRKAVVEAIHAAPDGETLAARNRGEIFRQFLCCML
                     ARIDITIGHAEREQPAPEHTGYRNADRLMDDIDLMRNALELAGARRLSDALLLPLRRE
                     VGVFRFSTVRLDVRENSMRVNQTLAAMFRLKFDGADPPAADSVEWKRWLLSELRTPCT
                     EPRLYEGLPADAAETLATFRTIARTREEIDRDAFGALILSMTRSAADILGVYLMAKEG
                     GLFADAAAVERCTLPIVPLLETIPDLRRAAPILKELLAVPLVQRSLHVQGKIQEVMIG
                     YSDSNKDGGYLTAHWELAKAQSSLTRLGSEHGVAIAFFHGRGGSVSRGGAPTGRAIAA
                     LPAGSIRGQFRVTEQGEVVSFKYANRGTAAYQIELLGASVVEHVLRSEREAALIPVHE
                     FQEAMEALSGTAWSAYRKLMEMPAMLAYLQDSSPLEELSLLNIGSRPARRTQATTLGD
                     LRAIPWVFAWTQNRHMLSGWFGVGSGIKAFLDVRKERGLALLKRMFRDARLFRLILDD
                     VERTLLQVDLSIAREYAMLVEDEAVREPIFSRIEEEYRLTCKMILCISGDAGIAERFP
                     QFRRRLARRLKTINQVSREQVHLLRQHRARSSDEVRTALLMSINCAAAGLGATG"
     gene            4327..>5296
                     /locus_tag="VE030_13240"
     CDS             4327..>5296
                     /locus_tag="VE030_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HZF24417.1"
                     /translation="MSFTNIEPATPRLHRSELAVPGSNTALFEKAAKSAADIVFLDVE
                     DAVAPDDKEQARNNIIQGLNEIPWGTKTMMVRINGLDTHYMYRDLVDIVEACPRLDMI
                     LIPKAGVAADVYAVDMMVTQIEAAKKRTKRIGFEVLIETALGMANVEAIAQSSKRLEA
                     MSFGVADYAASTRARTTVIGGVNRDSGVLTDKDADGKREYFWTDPWHAAQTRMLIACR
                     AYGLRPIDGPFGDFNDPDGFRAAANRAAVLGYEGKWAIHPSQVALCNEVYTPSAAEVT
                     KATRIMEAMAQAAREGRGAVSLDGRLIDYASIRMADALLKKAEAIGR"
BASE COUNT          994 a         1683 c         1698 g          921 t
ORIGIN      
        1 atctggatgc aacgatggtc gagatcaatc cactggtggt gaccaaggat aaccgggtgc
       61 tggcgctgga tgccaagatg tccttcgacg acaacgccat gttccgtcgt cccagggtca
      121 cagaaatgcg cgactccgcc gaagaggatc cacgcgaagc tcaggcagcg gagttcggtc
      181 tcaactacgt tggcttgagc ggggatatcg gctgcatcat caacggcgcc ggattagcga
      241 tggcgaccat ggacatgatc aagcacgccg gcggtgagcc cgccaacttc ctcgatgtcg
      301 ggggtggcgc gtcgccggag cgcgtcgcgg cggcattcaa tctcgtgctc tccgatcaga
      361 atgtgaaggc gatcctggtc aacatcttcg ccggcatcaa ccgctgcgac tgggttgcgc
      421 aaggcgtggt gcaggcggcc aaaagcctgc gcgtgccgct ggtcgtgcgg ctcgccggca
      481 ccaatgtcga ggaaggcagg cgcatcgtcc gcgattgcgg cctgcccatc atcacggccg
      541 acactctggc cgaggcggcg cataaagttg tcgcggcgtc gcgcggtgga gacgtcaaat
      601 gagcattctc atcgacgcaa cgacgccggt aatcttgcag ggcttcaccg gtgacaaggc
      661 gactttccac gccaaggaaa tgatcgaata cggcaccaag gtcgtgggcg gagtcacgcc
      721 gggcaagggt gggcagacgc atctggaccg tccggtcttc aatacggtga aagacgcggt
      781 gaaggccacc ggcgccgagg ccagtctggc ctttgtgcct ccgccttacg cggcggatgc
      841 gctgatggaa gccgccgacg cgggcttgag gctggtgtgc atcatcaccg acggcattcc
      901 cgcgcaggac atgatgcggg tcaagcgcta cctgcggcgc taccccaagc ccaagcgcac
      961 caccgtcgtg ggcccgaact gcgcgggcat catcagcgcc ggcaaggcga tgctcggcat
     1021 catgccgagc aacatctaca tccccggcaa cgtcggcgtc gtctcgcgct ccggcacgct
     1081 gggttatgag gctggtgcgc agatgaaggc gctcggcatc ggcattacga ccagcgtcgg
     1141 catcggcggc gatccgatca acggcagttc cttcctcgat cacctgatct tgttcgagga
     1201 ggatccggaa accaaggccg tgatcatgat cggcgaaatc ggtgggccgc aggaatcgga
     1261 ggcggcgaaa tgggtcaaag agaacatgac caaaccggtc gtcggttacg tggccgggct
     1321 gaccgcgccg aagggtcgcc gcatgggcca tgccggagcg atcatttccg ccgaaggcga
     1381 cagcgcctcg gaaaaggcgg aaatcatgaa gtcgtacggc cttttcgtcg cgcccagcgc
     1441 cgccgaactc ggctcgacgg tggcgctggc gctgagtcgg gcgggctgaa cgcgcgtggc
     1501 aaccgtctcg ccacgcagac ttgtccgtgc gacgccaagt cactgagcga ccgtcttgaa
     1561 cgatcccgcc ggcccggccg atcctgctgc tccctcacgc gagccgcccg gaaacttttc
     1621 gcgcgcggca ctgctgttcg acttgctggt cggcgtggtg cagcgctacg agccgccggt
     1681 cgcagccgtg ctgcgcgagg agcaaacgac cgagggcatg tccgcagtcc tgctcgcacg
     1741 cacgctgcag gcccaaggca tttggttcca gctgctcgcg atcgcagaac agaaccgcga
     1801 catgcgcaat cgccgcgagg ttgaacgcga acgcggctac gagcagttgc ggggcacctt
     1861 cgccgccgtg ttcgcatccg ctgcggccga ggggatcacg ggcgcggagg tccatcaggc
     1921 attgtcgggg ctacgtgtac ggccggtgat tacggctcat ccgaccgagg caaaacgcgt
     1981 aaccgtgctc gagcggcatc ggcgcattta cctgcgccta ctggatctgg aatcgccgcg
     2041 ctggacagag cgcgagcggc aggaactgat ccacgccacc ggcgacgaaa tcgaactgct
     2101 ctggctcacc ggcgaactca agctgtccaa acccacgatc gcgcaggaag tggcttgggg
     2161 cctgtatttc ttcaacgaaa atctctttga cgtggtcccg caaatgttgg ccaagatcga
     2221 gagcgcgttc gcgaatcaat ttcccggcga gccaatcgac gtcccgctgt tctttcagtt
     2281 cggctcctgg atcggcggtg atcgcgacgg caatccttac gtcaccagcg aagtcacgcg
     2341 acgcacgttg tgggacacac ggctggccag cttgcgccgc tatcgaatcc gcctactgga
     2401 tttagcgcgt aatctcagca tcgaggatca cgcgctggcg ctcccggatt cgttccgcaa
     2461 ggcggtggtg gaagcgattc acgcggcgcc ggacggcgag acccttgcgg cgcgcaatcg
     2521 aggcgaaatc ttccgtcagt tcctgtgctg catgctggcg cgcattgaca ttaccatcgg
     2581 gcatgccgaa cgcgaacagc ccgcacccga gcacaccggt taccgcaacg ccgaccgcct
     2641 catggatgac atcgacctga tgcgcaatgc gcttgagctg gcgggcgcgc ggcgtctgtc
     2701 cgatgctcta ctgctcccct tgcgccgaga ggttggcgtg ttccgcttca gcaccgtgcg
     2761 cctggacgtg cgcgaaaaca gcatgcgcgt caaccagacg ctggccgcca tgttccgtct
     2821 caagtttgac ggcgccgacc cacccgccgc cgattcggtg gaatggaagc ggtggctgtt
     2881 gtccgagctc cgtacgccgt gcaccgagcc tcgcctctac gaaggtctgc cggcggatgc
     2941 ggcggagacg ctggcgacct tccgcaccat cgcccgcacg cgcgaagaga tcgatcgcga
     3001 cgctttcggc gcgctgattc tgagcatgac gcgcagcgcg gccgacatct tgggcgtcta
     3061 tctcatggcg aaggagggcg gtctttttgc cgatgccgca gccgtcgagc gctgcacctt
     3121 gccgatcgtg cccctgctcg aaaccatccc cgacttgcgc cgggccgccc caattctcaa
     3181 ggagttgctg gcggtgccgc tggtgcagcg cagcctgcat gtgcagggca agattcagga
     3241 ggtgatgatc ggctactccg attccaacaa ggacggcggc tacctcaccg cgcactggga
     3301 actggcgaag gcgcagagca gcctgacgcg cttagggtcg gagcacgggg tggcgatagc
     3361 gttcttccac ggccgcggcg gctcggtgag ccgcggcggc gcgccgaccg gacgcgccat
     3421 agccgccttg cctgcgggtt cgatccgtgg ccagttccgc gtcacggaac aaggtgaggt
     3481 cgtctccttc aagtacgcga accgcggcac ggcggcgtat caaatcgaac tgttgggtgc
     3541 atcggtggtc gagcatgtgc tgcgctccga acgcgaggca gcgctgattc cggtgcatga
     3601 attccaagaa gcaatggaag cgctttccgg cactgcatgg tccgcctacc gcaagctgat
     3661 ggaaatgccg gcgatgctcg cctatctgca ggattccagt ccactggagg aattgtcgct
     3721 gctcaacatc ggctcgcggc cggcgcgccg tacccaggcc acgacgcttg gcgatctgcg
     3781 ggctattcct tgggtgttcg catggacgca gaaccggcac atgctgtccg gctggttcgg
     3841 cgtcggcagc ggcatcaagg cctttctcga cgtgcgcaag gagcgcgggc tggcacttct
     3901 caagcgcatg tttcgcgacg cccggctgtt tcggctgatt ctcgacgacg tggagcgaac
     3961 tctgctgcaa gtcgatctgt cgatcgcacg cgagtacgcc atgctggtcg aggacgaggc
     4021 cgtgcgcgag ccgatctttt cgcgtatcga agaggaatac cgtctgactt gcaagatgat
     4081 tctgtgcatc tccggcgacg ccggcatcgc cgagcgcttt ccgcagttcc ggcggcgcct
     4141 cgcgcgccgg ctgaagacca tcaaccaggt cagccgcgaa caagtgcact tgctgcgcca
     4201 gcatcgagcc cggagcagcg acgaagtgcg caccgccctt ctcatgtcga tcaattgtgc
     4261 cgccgccggt ctcggcgcga ccgggtagcg ttgccgtatc caagcgtttc gaacggagag
     4321 tcacctatga gcttcaccaa catcgagcct gccacgccgc gtctgcatcg ctccgaactt
     4381 gcggtcccgg ggtccaacac ggccttgttc gaaaaggccg cgaaatccgc ggccgacatc
     4441 gtcttcctcg acgtcgagga tgcggtcgcg ccggacgaca aggaacaagc acgcaacaac
     4501 atcatccagg gtctcaacga aatcccctgg ggtactaaga ccatgatggt ccgcatcaac
     4561 ggtctcgaca cgcactacat gtatcgcgac ctggtcgata tcgtcgaagc ctgcccgcgt
     4621 ctggacatga tcctgatccc taaggcgggc gtcgctgccg acgtgtacgc ggtggacatg
     4681 atggtgaccc agatcgaggc agcgaagaag cgcaccaagc gtatcggctt cgaagtgttg
     4741 atcgagaccg cgctcggcat ggcaaatgtc gaggccatcg cgcagtcgag caaacggctg
     4801 gaggcgatgt cgttcggcgt ggccgactac gccgcgtcca ctcgcgcccg cacgacggtg
     4861 atcggcggag tcaaccgcga ctccggagta ctcaccgaca aggacgccga tggcaaacgc
     4921 gaatatttct ggactgatcc ctggcacgcg gcacagactc ggatgctcat cgcgtgccgc
     4981 gcctacggat tacgccccat cgacggcccg ttcggcgact tcaacgaccc cgacggcttc
     5041 cgcgcagccg caaaccgggc ggccgtgctt ggctacgagg gtaaatgggc tatccaccct
     5101 tcccaggtcg cattgtgcaa cgaggtgtac acaccatccg ccgccgaggt gacgaaggcg
     5161 acgcgcatca tggaggcaat ggcgcaagcg gcgagggaag ggcgcggcgc cgtatcgctc
     5221 gacggccgtt taatcgacta cgccagcatt cgtatggccg atgccctatt gaaaaaggca
     5281 gaagcaatcg gacgcc
//
LOCUS       DASQOP010000340        13894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2191242, whole genome shotgun sequence.
ACCESSION   DASQOP010000340 DASQOP010000000
VERSION     DASQOP010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13894
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2191242"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="VE030_13245"
     CDS             <1..777
                     /locus_tag="VE030_13245"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZF24418.1"
                     /translation="PGAPEHAVPVKPVEYFVPAWDEICKRTVREQAPRRIAVVGGGAA
                     GVEMLLAMQYRLRTLAPAGEISFELVSDTGSILLTHNSKVRTIFMRVLERRGVTLHLG
                     RPVERVDSGRLQLRGGGGIEADAIVWATGASAPLWPRSVGLVTDERGFILVNGRLQSV
                     SHPQVFASGDIASVRDHPRPKSGVYAVRAGPRLAENLRRALRGDQLIEWKPQKEALAL
                     ISTGDRYAVASRGNLALEGKWVWQWKNWIDRRFMKRYEMP"
     gene            892..1296
                     /locus_tag="VE030_13250"
     CDS             892..1296
                     /locus_tag="VE030_13250"
                     /inference="COORDINATES: protein motif:HMM:NF037472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rap1a/Tai family immunity protein"
                     /protein_id="HZF24419.1"
                     /translation="MTRALLILLLLFPIATTADPLGPGGIAKESGEQLLARCEPALRM
                     IEAGGDAGLSAAERIEASSCIGFVDGFIWGHAWSAWRERSDMFFCPPELFSAKQGVPA
                     LMSYLRAHPDRLIQRAHLLLFAAFNSAYPCQP"
     gene            1513..2118
                     /locus_tag="VE030_13255"
     CDS             1513..2118
                     /locus_tag="VE030_13255"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HZF24420.1"
                     /translation="MKSIGRIFLTGILTILPILATLYLVVWLFAAVERFLGKQLLLLL
                     PDEYYRAGMGLLAALALIFLIGLLMRAWLFRQLVKAGENLLLKIPLIKVVYRSLKDLF
                     GLFSDDKNREALQVVSVQLPGTDMRLLGFLTRSDFSDMPKGVGNEDEVAVYLPMSYQV
                     GGYTVLIPRKRVTPVPMSREEAMRFVLTAGLKIGEAVKGEG"
     gene            complement(2243..3109)
                     /locus_tag="VE030_13260"
     CDS             complement(2243..3109)
                     /locus_tag="VE030_13260"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HZF24421.1"
                     /translation="MIDMQRVGEQTDVLGEGPVWDVEEQALYWVDIRKPAVRRYDGGN
                     RKVSTWTTPEMVGSLAVRKQGGLLLALKSSLAFFDPQFGAIRRVASPEADRPSQRFND
                     GKCDRQGRFWAGTMNDADRQPDGTLYRFDRSGCVAVESGLTIPNSLCWSPDGRTMYFS
                     DSWRRTICAYDFDPAKGQAANKRPFATTAAPAIPDGATIDAEGCLWCAEYDGWRITRF
                     APDGRRIRSIDLPLQRPTSCMFGGPNLDTLFVTSATQRLSSEELAKQPLAGALLAMDV
                     GVRGLPETRFDG"
     gene            3238..3543
                     /locus_tag="VE030_13265"
     CDS             3238..3543
                     /locus_tag="VE030_13265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HZF24422.1"
                     /translation="MDRFLITVDFYLLPAALDPFLALIKENARKSLTEEPGCHRFDVL
                     VQKGMPDHILLYEIYQNRAAFDVHMKSRHFADFNSASAPYVKSKKVVEYEYVNDEGE"
     gene            complement(3782..4714)
                     /locus_tag="VE030_13270"
     CDS             complement(3782..4714)
                     /locus_tag="VE030_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HZF24423.1"
                     /translation="MDIVSLGEPLYEFSQIPGESGRYLQGFGGDTMNCAIAAARQGAK
                     AGYITRLGDDEFGRQFLQLWRAEGLDISGVGIDPQAHTAVYFISHGADGHVFSYLRQG
                     SAASRIRPADLPLELIRDATFFHTSGISQAISDTACDTVFAAIEAARGAGVRIAYDSN
                     LRLRLWPLARARAVIRATIALADYFLLSMEDAEALCGERDADAILDWCHEAGAKVVAL
                     KLGAAGVVGSDSKRREKLAGHAVNCVDATGAGDCFAGALLARMAVGDDFWEGLRYANA
                     AAALATTGFGAVAPLPRPDAVRRLLSGFASQGRR"
     gene            complement(4726..5364)
                     /locus_tag="VE030_13275"
     CDS             complement(4726..5364)
                     /locus_tag="VE030_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HZF24424.1"
                     /translation="MNSTTEKLIALRVIPVLRLATRPGAESAIDCLVEAGFKTVELTL
                     TTPGAIDLIASLRRRAGVDFLVGAGTVLDLDTAKACLEAGADYLVSPGVVAGMGKLAN
                     DAGRAALLGGFTPTEVLAAWREGAAVVKVFPAATGGPAHLAALHAIYPQIPLCPTGGV
                     SAANLLDYFKAGAAVVGIGNNVIDQKALADGDRARVIGHARSFLELAKRTGA"
     gene            5577..7943
                     /locus_tag="VE030_13280"
     CDS             5577..7943
                     /locus_tag="VE030_13280"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZF24425.1"
                     /translation="MKRPESCMLIDESSPQGTSPEAYLSLLQTVGRILADAPSLEDAL
                     DRIIRAVCATLHWDYGARWQEDEQTHQFACTQMWHAPFLDNTAFVEMIRSTSFMPGPE
                     GLLRTVLKTRQPFLVEDTSAAPGLRRAQAAMEAGLRSAFAFPLLAGGRTLGALEFFSR
                     QPQLQDERLLMTGATIGTLVGEAVARRQSEERYRQLVELSPDAVLGHCEDRSVFAKRA
                     AESMLREQERAMSTLLRNLPGMAYRCRLDDHWTLEFVSEGAMHLTGYPAGDLVENRKI
                     SFIQLVHPDDRGWVVDEIRDKLERGDRFVIEYQIICADGAVKWVSEKAQAVRDAAGAI
                     VALEGIIDDVTERKRFEERLAILAQYDVLTGLPNRSLFYDRLRQAIVRARREQTMVGL
                     LFLDLDRFKQINDSLGHAAGDRVLKTIAERLQGFLREIDTIARLGGDEFTVIIEGVND
                     PAQVSSVAEKIRAALSDPIVLDGRDMLVSASVGITLYPRDAEDIEHLVKNADIAMYHA
                     KNRGGRQQFQFYDKGMAPFAAEQLEMEAKLKRALENGEFQLQYQPAVDVKNGHIIGME
                     VLVRWKSPEGLVPPGKFIPVAEESGLIVDIGRWVLKAACAQLRAWQKEGLPPLRLAAN
                     LSPPQVKQSNLLEMISEILRETGLGADHLELEITENTIMERSGDTIATLRRLEELGVH
                     LSVDHFGTGYSSLAYLKRFPVHRLKIDKSFIADITVDSDNAAIVSAMIAMAKNLGLGV
                     IAEGVETKAQLEFLRAAGCDTYQGYYFSVPLPANAFAELVRRQVQQKQ"
     gene            complement(8070..8333)
                     /locus_tag="VE030_13285"
     CDS             complement(8070..8333)
                     /locus_tag="VE030_13285"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HZF24426.1"
                     /translation="MNIFVGNLAPEVNETDLAELFKPFGEVKSTEVKRELFSGVGKGF
                     GFVEMPGRNHSLAAIAGLNGKEFKGQPLRVNEAHEFPARGRRR"
     gene            complement(8412..9791)
                     /locus_tag="VE030_13290"
     CDS             complement(8412..9791)
                     /locus_tag="VE030_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZF24427.1"
                     /translation="MTTPFEDLGLRAELIRAVADQGYTQPTPVQAQAIPVILQGRDVL
                     AGAQTGTGKTAGFTLPLLQLLSAKPASGNASRPIRALILTPTRELAAQVEEAVRTYGS
                     HLRLRSATVYGGTSMNQQIRALRQGVDILVATPGRLLDHLQQRTANLSNVEYLVLDEA
                     DRMLDMGFIRDIRRILTSLPAKRQNLFFSATFSKEIRGLADSFLHAPVEVAVERIDAT
                     SNLISQVVHPVDKDRKRALLAHLITSGDWRQVLVFSRTKHGASRLAEQLDRDGITADA
                     IHGNKNQSQRTRVLSQFKAGNLRVLVATDIAARGLDIELLPHVVNYELPNVSEDYVHR
                     IGRTGRAGADGCAVSLVCSEESGFLRDIERLMQRRVERVVIEGFEPSSGMARFDSAPP
                     PAARNARSQQRRPQRNGQGPANGARGSNGRNGGRPFGGQPSRRSETQQRRSQGGRGRP
                     GATLLGGNR"
     gene            complement(10159..13014)
                     /locus_tag="VE030_13295"
     CDS             complement(10159..13014)
                     /locus_tag="VE030_13295"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HZF24428.1"
                     /translation="MKANDPHLSLPAQQVSIDVLLEKYAKGGEKSVRDVRLRVARALA
                     AVEKEPAKWEPVFLEALESGFIPGGRINSAAGTQLAATLINCFVQPVGDSVSETVDGK
                     PGIYVALMEAAETMRRGGGVGYDFSAIRPHGALVRGTQSSASGPVSYMRVFDQSCETV
                     ESAGARRGAQMGILRCDHPDIERFIHAKDDGSLSNFNVSVVVTDAFMRTVEADGDWEL
                     VHKVQPSGPLPGPLPKGEGVSRQRADGMWVYRTVKARDLWKQIMDSTYDHAEPGVIFI
                     DQANAENNLSYCETIEASNPCAEQMLPAYGCCCLGSINLTPFVSHPFTPKARFDFESF
                     AKVTKVAVRMLDDVLDATVWPLPQQKQEADDKRRIGLGFTGLGDALIMLGLRYDTEEA
                     RAMAARIAEHMRDDAYAASVDLAQEKGAFPLLDADQYLAAPRFASRLPQALKQAIRRH
                     GLRNSHLLSIAPTGTISLAFADNAANGIEPPYSWTYQRKKRSHDGTQKVYDVEDHAWR
                     LYRHLGGNTDKLPPQFVTALEISALDHMRMVAAVAPFVDSAISKTVNVPEDYPYENFK
                     DLYFEAWKSGLKGIATYRPNSVLGSVLSVTTHEEAPNDLDTSDADRRIRLEAAPTPAL
                     SSLRWPSRPRLAAGNPAWTYMVESPFGQFAIFVGHVDNGDPHAFEVWINGAEQPRGIG
                     AVAKTLSMDMRAQDKAWLRMKLDILSRTAGDDAFDCPLPPDGKPKRVPSLVAAFARIV
                     AWRVEQLGALTARDGRSPVLDALFAKKEPKTGTDGTMSWSVDVFNQNSGDDFVLILKE
                     LVLPSGQRRPYSVWMAGAYPRSLDGLCKLLSLDMRVIDPAWIGMKLRKLLNYAEPLGD
                     FMARVPGENRQRNWPSTVAYVARLIIHRYAMLGVLDERGYPVDEMGVLEIPEAARERE
                     ELRMLPGRRCTECGNTAVIRKDGCEFCTACGAIGACG"
     gene            complement(13032..>13894)
                     /locus_tag="VE030_13300"
     CDS             complement(13032..>13894)
                     /locus_tag="VE030_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24429.1"
                     /translation="GFWDAIRKLFAATTEARMRGYEPSRFSFNTSGGRCDGCEGQGLK
                     TIEMSFLPDVKVTCEVCGGARFNQETLAVRYKGKSIGDVLMMSVDEAVEFFSAHASIH
                     HALRLLQDVGLGYLTLGQQSPTLSGGEAQRIKLVTELSKVRSDVLPLSAEEVRSVSPR
                     ASRLAPRAKHTLYVLDEPTVGLHMADVEKLIRVLHRLVDAGNTVVVIEHNLDVMADSD
                     WLIDLGPEGGDGGGRVVVMGPPESIARYPATSHTAKVLADFLRERGRPEPAMPPREPR
                     RLRGAEVSLK"
BASE COUNT         2511 a         4437 c         4386 g         2560 t
ORIGIN      
        1 cccggcgcgc ccgagcatgc cgtgcccgtg aaaccggtgg agtatttcgt tcccgcctgg
       61 gacgagatct gcaagcggac cgtccgcgag caggctccgc ggcggatcgc tgtggtcgga
      121 ggcggcgccg ccggcgtcga aatgctgctg gcgatgcagt atcgcctgcg cacgctggcg
      181 cccgcgggcg aaatcagctt cgagctggta agcgacaccg ggagcatact gctcacccac
      241 aacagcaagg tgcgcacgat tttcatgcgc gtgctcgagc ggcgcggcgt gaccctgcac
      301 ctgggacgcc cagtcgagcg cgtggacagc ggcaggctgc agctgcgcgg tggcggcggc
      361 atcgaggccg atgcgatcgt ctgggcgacc ggtgcttcgg cgccgttgtg gccgcggtcg
      421 gtcggcctgg tcaccgacga acgtggattc attctggtga acgggcgtct gcaatcggtc
      481 tcacatccac aggtgttcgc ctcgggcgat atcgccagcg tcagggatca tccgcgcccc
      541 aaatccggcg tgtatgcggt tcgcgccgga ccgcggttgg cggagaacct gcggcgggca
      601 ttgcgtggag atcagctcat cgaatggaaa ccgcagaagg aggcgctggc gctgatcagc
      661 accggtgacc gctacgcggt cgcgtcccgc ggcaatctcg cactggaggg caaatgggta
      721 tggcaatgga agaactggat cgacaggaga ttcatgaaac gctatgaaat gccgtgacgg
      781 tcgtgactgt cgtgaaaaac gtaaaaatcg tctgaaagct ctacccgtga gcgttcactg
      841 catttacggc atttgcgaca gtcacgaaag tcacgatcca cgatcatcac catgaccaga
      901 gccctcctaa ttctcctgct gctatttcca atcgccacca ccgccgaccc ccttggtcca
      961 ggcggcatcg ccaaggaaag cggcgagcag ttgcttgcgc gctgcgaacc ggcgctgcgg
     1021 atgatcgaag cgggcggcga cgccggtctg agcgccgcag aacgcatcga ggcgtcatcg
     1081 tgcatcggct tcgtggacgg cttcatctgg ggccatgcct ggtcggcgtg gcgcgagcgc
     1141 agcgacatgt ttttctgccc accggagttg ttcagcgcca agcaaggtgt tccggcgcta
     1201 atgtcatatc tgcgtgcgca tccggatcgc ctgatccagc gcgcccacct gctgctcttc
     1261 gctgcgttca acagtgcata tccgtgccag ccgtgattcc ggatgctcgt gcccgactcc
     1321 agggtcgcat ccccgtgaac ggggcccctg ctccgccttc cgcgggccat ccctcccgac
     1381 tgatccagcg cgcccacctg ctgctcttcg ctgcgttcaa cggccaacct atccgcgcaa
     1441 accgtgacgc gcctatgctc gtgtctgata cattgcagca gtccgcttca caacacttta
     1501 attggagtac gaatgaaatc cattgggaga atcttcctga ccgggatcct gacgatcctg
     1561 cccatcctgg caaccctata tctggtggtc tggctgttcg cggcggtgga acgcttcctc
     1621 ggcaagcagc tgctgctcct gctgccggac gagtactacc gtgccggcat gggcctgctc
     1681 gccgcattgg ccctcatttt tcttatcggc ctgctgatgc gggcctggct gttccggcag
     1741 ttggtcaagg ccggcgagaa ccttctgctg aagattccgc tgatcaaagt ggtgtacagg
     1801 tcgctgaagg acctgttcgg cctgttttcg gacgataaga acagagaggc gcttcaggtg
     1861 gtttcggtgc agcttccggg caccgacatg cgcctgctcg ggttcctgac ccgcagcgat
     1921 ttctccgaca tgcccaaagg cgtcggcaac gaggacgagg tggcggtcta tctgccgatg
     1981 agctaccagg tcggcggcta cactgtgctg atcccgcgca aacgggtcac tccggtgccg
     2041 atgtcgcgcg aagaggcaat gcggttcgtg ctgaccgcgg gactgaaaat tggtgaagcc
     2101 gtgaagggtg aagggtgaag ggtgactgca ggattttatg tgactcttgt tttgtttcct
     2161 ggctttcttt gcgtcctttc ttgctcttac tctgcgtcct ctgcgtcaac gctcctgatg
     2221 ccggctttcg gccggctgct tttcatccgt caaagcgggt ctcgggcagt ccgcgcacgc
     2281 ctacatccat tgccagaagc gcgcccgcca gtggctgctt cgccagctcc tcggaactga
     2341 gtctctgcgt ggcgctggta acgaaaagcg tgtccaggtt cgggccgcca aacatgcaac
     2401 tggtcgggcg ctgcaacggc aaatcgatcg agcggatgcg cctgccatcg ggcgcaaaac
     2461 gggtgatgcg ccagccgtcg tactcggcac accacaggca gccttcggcg tcgatggtcg
     2521 caccgtccgg aattgccgga gcggcggtgg tcgcgaacgg gcgcttgttc gcggcctgcc
     2581 cttttgccgg atcgaaatcg taggcgcaga tcgttcgccg ccatgaatcg gagaagtaca
     2641 tcgtccgccc gtcggggctc cagcacaggc tgttcgggat ggtgaggccc gactcgacgg
     2701 caacgcatcc cgatcggtcg aaccggtaca gcgtgccgtc gggctggcga tccgcgtcat
     2761 tcatggtgcc agcccagaag cgcccctggc gatcgcattt gccgtcgttg aagcgctggc
     2821 tgggccgatc ggcctcgggc gacgcgacac gccgaatggc gccgaattgc ggatcgaaga
     2881 acgccaagct cgactttagc gcaagcagca gcccgccctg cttccgcacc gccagcgagc
     2941 ccaccatttc cggcgtcgtc caagtgctca ctttgcggtt gccgccgtca taacgccgta
     3001 ccgcgggttt gcggatgtcc acccagtaca acgcctgttc ttccacatcc cagaccggcc
     3061 cctctccgag cacgtcggtc tgctccccca ctcgttgcat gtcgatcatg gttgcgtcgg
     3121 agtcggctta tcagtatggt gccgggaaac cgtggcaggt gattcgtggt actcggcacg
     3181 gttcgatttt accggcaatg atccacgaat aacgaaccgc gaaccacgga agtctccatg
     3241 gaccgttttc tcatcaccgt cgatttctat cttctccccg cagcccttga tccgttcctg
     3301 gccctgatca aggagaacgc gcgcaagtcg ctgaccgagg agcccggctg ccaccgcttc
     3361 gatgtgctgg tgcaaaaggg catgccagac catatcctgc tctatgaaat ctaccagaac
     3421 cgtgcagcct tcgacgtaca tatgaagtcc cggcatttcg cggacttcaa ttcggcgtcg
     3481 gcgccgtacg taaagagcaa gaaggttgtc gagtacgaat acgtaaacga cgaaggggaa
     3541 tgaaaagagg gaatttcgta tgcggacgat gggggcgcgt cgcgcggcgt ttcatgaatt
     3601 tcccaatcac gtacaatcgc gattgctgat ttaccgcagg ggaatgcggc tgttagaggg
     3661 agatgtccga tgacttggct cgacgcgtgg ttggcaggtg gcgtggtctt actggtcgtc
     3721 ctgatcgtat tgcgcaaaaa agggcgctga ggcacccgtg tccggttgcc agcatccgca
     3781 atcatcggcg gccctgcgaa gcgaaaccgg atagcaatcg gcgcaccgca tccggcctgg
     3841 gcaacggcgc gaccgctccg aaaccggtag tcgcaagggc cgcggcggcg ttggcgtagc
     3901 gcagcccttc ccagaagtcg tcacccaccg ccattcgcgc cagcagcgcg ccggcaaaac
     3961 aatcacccgc cccggtggca tccacgcagt tgaccgcgtg gccggcgagc ttttcgcgcc
     4021 gcttcgaatc gctccccacc acccccgccg cacccagctt cagtgcgacg acttttgcac
     4081 cggcctcatg gcaccagtcg agtattgcgt ccgcatccct ctcgccgcat agcgcctccg
     4141 cgtcctccat gctgagcagg aaatagtcgg caagagcaat ggtggcacga atcaccgcgc
     4201 gggcacgggc aagcggccac agccgaagcc gcaggttgga atcgtaggcg atcctgaccc
     4261 cggcgccccg cgccgcttcg atcgcggcga acaccgtatc gcaggcagtg tcgctgatcg
     4321 cctggctgat tccggatgta tgaaagaacg tcgcatcccg aatcagctcg agcggtaagt
     4381 ccgcgggccg aatgcggctc gccgccgagc cctggcgcag gtagctgaac acgtgaccgt
     4441 ctgcgccgtg cgagatgaag taaaccgcgg tgtgcgcttg cgggtcgatg cccacgccgg
     4501 agatgtccag tccctcggct cgccatagct ggagaaactg ccggccgaac tcatcgtcgc
     4561 cgagccgcgt gatgtagccc gctttcgcgc cctgtcgcgc ggcggcgatc gcgcagttca
     4621 tggtatcgcc accgaaaccc tgcagatagc ggccgctctc tcccggtatc tggctgaact
     4681 cgtacaacgg ctcgcccagg gacacaatat ccatgcgtgc caccgtcagg cccccgtgcg
     4741 tttcgccagc tcgaggaagc tccgcgcatg accgatcacc cgcgcgcgat cgccgtccgc
     4801 cagcgctttc tggtcgatca cgttgttgcc gatgccgacc acggccgcgc cggccttgaa
     4861 gtaatcgagc aggttagcgg ccgacacgcc acccgtcggg cagaggggaa tctgaggata
     4921 aattgcgtgc agcgcggcga ggtgcgcggg accgccggtc gcggccggaa aaaccttcac
     4981 gacggccgcg ccttcacgcc acgccgccag cacctcggtc ggggtaaagc cgccgagcag
     5041 tgccgccctg ccggcatcgt tggccagctt gcccatgccc gccaccacgc cgggagaaac
     5101 caggtagtcc gccccggctt ccaggcaggc ctttgcggtg tcgaggtcga gcacggttcc
     5161 ggcgccgacc agaaaatcca caccggcacg acggcgcaga gatgcgatga gatcgattgc
     5221 acccggtgtg gtcagcgtca gttcgaccgt cttgaatcct gcctcgacga gacagtcgat
     5281 ggcggattcc gcgcctgggc gcgtggccag ccgcaatacc gggatcacgc gcagtgcgat
     5341 cagcttttcg gttgtcgaat tcatgttctt cgggtcgcgg aatccacttc cccgatcgca
     5401 ggctcggggc gaacctgggt cggcgcattg cgcaacccac ttaaggctga acgggcgatt
     5461 gtaaaagcaa agccagccac tgagtagcac gcactgcagc ctcagtccac attttggcgt
     5521 ccggttccgg ccgcccattg caccgccatc tgatttagac tccgggttcg taccgcatga
     5581 agcgaccaga gtcctgcatg ctgatcgacg aatcctctcc ccaaggcacc tcgcccgagg
     5641 cgtacctttc gctgctgcag accgtgggcc ggatccttgc agatgcaccc tcgctggagg
     5701 atgcactgga ccggatcatt cgggccgttt gcgcgaccct gcattgggac tacggcgccc
     5761 gatggcagga agacgaacag acgcatcagt tcgcctgcac gcagatgtgg catgcgccct
     5821 tcctggataa cactgcgttc gtggagatga ttcgcagcac atcctttatg ccgggccccg
     5881 agggtttgct gcgcacggtg ctcaagacac ggcaaccgtt tctggtcgaa gacacctccg
     5941 ccgcgcccgg attgcgccgc gcgcaggctg ccatggaggc cgggctgcgc tccgccttcg
     6001 cttttccgct gctggcgggc ggccggacgc tgggcgcgct ggagttcttc agccggcagc
     6061 cgcaactgca ggacgaacgc ctgctcatga ccggcgccac catcggcacg ctcgtcggcg
     6121 aggccgtcgc gcgcagacag tccgaggagc gctatcggca actggttgaa ctgtcgccgg
     6181 acgccgtcct gggtcactgc gaggaccgct ccgtgttcgc gaagcgggcc gccgagtcga
     6241 tgttgaggga acaggaacgg gccatgtcca cgctgctgcg caacctgccc ggcatggcct
     6301 accgctgccg gctcgacgac cactggaccc ttgagttcgt cagcgaaggc gccatgcacc
     6361 tgaccggcta tccggccggc gacctggtgg aaaatcgcaa aatttccttc attcaactgg
     6421 ttcatcccga cgatcgcggc tgggtggtgg acgagattcg cgacaagctc gagcgcggcg
     6481 accgtttcgt gatcgagtac cagatcatct gtgcggacgg cgcggtgaag tgggtgtcgg
     6541 aaaaggcgca ggcggtccgc gatgccgccg gcgcgatcgt cgcgctggaa ggcattatcg
     6601 acgacgtaac cgaacgcaag cgcttcgagg agcgccttgc cattctcgcc cagtacgacg
     6661 tgctcaccgg cctgcccaac cgctcgctgt tctacgaccg gctgcgccag gccatcgtgc
     6721 gtgcgcgccg cgaacaaacc atggtgggct tgctgtttct cgatctcgac cgcttcaagc
     6781 agatcaacga cagcctcgga cacgccgccg gcgatcgcgt cctgaagacc atcgcagagc
     6841 ggctccaggg tttcctgcgc gagatcgata ccatcgcccg gcttggcggc gacgaattca
     6901 ccgtcatcat cgagggcgtg aacgatccgg cgcaggtgtc ctccgttgcg gaaaagatca
     6961 gggccgcgct atccgatccg atcgtgctcg acgggcgtga catgctggtc tccgcgagcg
     7021 tgggcatcac gctctacccg cgcgacgcgg aagacatcga gcacctggtg aagaacgccg
     7081 atatcgcgat gtatcacgcc aagaatcgcg gcggccggca gcagttccag ttctacgaca
     7141 aaggcatggc gccttttgcc gcggagcagc tcgagatgga ggcgaaattg aagcgcgcgc
     7201 tggagaacgg ggaattccag ctgcagtacc agccggcggt ggatgtgaag aacgggcaca
     7261 tcattggcat ggaagtgctg gtgcgctgga agagcccgga aggactggtg cctcctggaa
     7321 aattcatccc cgtggcggag gaatccggcc ttatcgtgga catcggcagg tgggtgctga
     7381 aagcggcatg cgcgcagttg cgtgcgtggc aaaaggaagg gctgccgcca ttgcgcctgg
     7441 ccgcgaactt atcgccgccg caggtcaagc agagcaacct gctggagatg atcagcgaga
     7501 tcctgcgcga aacggggctt ggggcggatc acctggaact ggaaatcacc gagaacacga
     7561 tcatggagcg atccggcgac accatcgcca cgctccggcg tctcgaggaa ctgggcgtgc
     7621 atctgtccgt cgaccacttc ggcaccggct actcgagcct ggcctatctc aagcggtttc
     7681 cggtgcaccg gctcaagatc gacaagtcct tcatcgccga tatcaccgtg gacagcgaca
     7741 acgcggcaat cgtgagcgcc atgatcgcga tggcgaaaaa cctcggcctc ggtgtaattg
     7801 cggaaggcgt ggaaaccaag gcccagctgg agtttctgcg ggcggcaggt tgcgatactt
     7861 accagggtta ctacttcagc gtgccgctgc cggcgaatgc ttttgccgaa ctcgtcaggc
     7921 gacaagttca gcaaaaacag tgataggtga caagggaagg gtgaagggtg aagggtgaag
     7981 ggtgaagggt gacgggtgac gcttcgataa gaaaagggcc gcatcgcggc cctttcagtt
     8041 cttacctcgt cacgatagtc acggcatttc taccgccttc ttcccctcgc agggaattcg
     8101 tgcgcttcgt tgacgcgcag cggctgcccc ttgaattcct tgccgttcag tccggcaatg
     8161 gcagcgagcg agtgattgcg gccgggcatc tcgacgaagc cgaaaccctt tccgacaccg
     8221 gaaaacagtt ctcgcttcac ttccgtcgat ttcacctcgc cgaagggctt gaacagttcg
     8281 gcaaggtcgg tttcgttgac ttccggtgca aggttgccga cgaagatgtt catgtcattc
     8341 tcctctctgg tttcaaagaa acggaagcgc cgcgccgaag gcggtgcctc ggttcagcgc
     8401 gaaccgcgcg atcagcgatt gccgccaagt agagttgccc ccggccggcc ccggccgcct
     8461 tggctacggc gctgctgcgt ttcggaacgc cgggaaggct gcccgccgaa cggcctgccg
     8521 ccgttgcggc cgttgctgcc gcgggcaccg ttggccggac cttgcccatt gcgttgcggg
     8581 cgccgctgct gcgaacgcgc attcctggct gcaggcggcg gcgcagaatc gaacctcgcc
     8641 atgcctgagc tgggctcgaa accctcgatc accacgcgtt cgacacgacg ctgcatcagg
     8701 cgctcgatat cgcgcaggaa gccgctctcc tcgctgcaca ccagggacac cgcgcagcca
     8761 tcggctccgg cgcggccggt gcggccgatg cggtgcacgt agtcctccga aacattgggc
     8821 agttcgtaat tcaccacgtg cggcagcagc tcgatgtcga gtccgcgggc ggcgatgtcg
     8881 gtcgccacca gcacgcgcag gttcccggcc ttgaactgac tgagcacccg ggtacgctgg
     8941 ctctggttct tgttgccgtg gatggcatcg gccgtgatgc cgtcgcgatc cagttgctcc
     9001 gccaggcggc ttgcgccgtg cttggtgcgg gagaacacca ggacctggcg ccagtcgccg
     9061 ctggtgatca gatgcgccaa cagcgcgcgc ttgcggtcct tgtccaccgg atgcacaacc
     9121 tgcgaaataa ggttcgacgt ggcatcgatt ctctccactg caacctccac cggcgcatgc
     9181 aggaaggaat cggcaagtcc gcggatctcc ttcgagaagg tagcggaaaa gaacaggttc
     9241 tgccttttcg cgggcagcga ggtaaggatg cgccggatgt cgcggatgaa gcccatatcg
     9301 agcatgcgat cggcctcgtc gagcaccagg tattcgacgt tggacagatt cgcagttctc
     9361 tgctgaaggt ggtccagcaa tcgccccggt gtggcgacca ggatatccac gccctggcgc
     9421 agtgcgcgga tctgctggtt catgctggtg ccgccgtaaa cggtcgcgga gcgcagacgc
     9481 agatggctgc cgtaagtgcg aacggcttcc tcgacctgcg cggccagttc acgcgtcggg
     9541 gtcaggatca gggcacggat ggggcgggat gcgtttccgc ttgccggctt cgccgacagc
     9601 aactgcaaca gcggcagggt gaagcccgcg gtctttccgg tgccggtctg ggcgccggcg
     9661 agaacgtcgc ggccctgcag gatgaccggg atggcttgcg cctgcaccgg ggtaggttgc
     9721 gtgtaaccct gatcggcgac agcgcggatc agctcggcac gcaggccgag gtcttcgaat
     9781 ggcgtagtaa tagatatctc ctgaaacagc ctgcggaagc catgcttccg aaccggttca
     9841 ggcagaatcg actagggttg aaaacttcgg ggaaaaacga tcaccacgaa agggctgaga
     9901 cgcggaccga ttgacgcgaa ccaaaatcct tggcgcgcac tatacaggcc tggcacccga
     9961 atgtctcgca ctatttaggg gcgagggctg agagctgagg gacgaggaac gaggggcgag
    10021 gggcgagggg aaaaagcaga agtagaagca tcgcattgca accggggcaa cgctattcaa
    10081 tccatgaacc aaaaaataaa ctctccacat ccatccctcg tccctcttcc ctcgtccctc
    10141 gtccctattt ctttaccctc agccgcacgc gccgatcgcg ccgcaagcgg tgcagaactc
    10201 gcagccatcc ttcctgatca ccgcggtgtt gccgcactcc gtgcagcgcc tgccgggtag
    10261 catccgcaat tcttcgcgtt cgcgtgcggc ctccgggatc tcgagcacac ccatttcgtc
    10321 cacgggatag ccgcgctcgt cgagcacgcc gagcatggcg tagcgatgaa tgatgagccg
    10381 cgccacatag gccacggtgc tcggccagtt ccgctggcga ttttcgccgg gaacacgcgc
    10441 catgaaatcg cccaggggct cggcataatt gagcaatttg cgcagcttca tgccgatcca
    10501 tgccgggtcg atcacacgca tgtccagcga cagcagcttg cacagcccgt cgagcgagcg
    10561 cggataggcg cccgccatcc acaccgaata cggccggcgc tggcccgaag gcagcaccag
    10621 ctccttgaga atcagtacga agtcatcgcc agaattctgg ttgaagacgt ccaccgacca
    10681 ggacatggtg ccgtcggtgc cggtcttggg ttcctttttc gcgaacaacg cgtccagcac
    10741 cggcgaacgc ccgtcacgcg cagtgagtgc gcccaactgc tcgacgcgcc aggccacgat
    10801 gcgggcgaaa gcggccacca ggctcggcac gcgcttgggc ttgccgtccg gcggcagcgg
    10861 gcaatcgaag gcatcgtcgc ccgcggtgcg cgacaggatg tcgagcttca tgcgcaacca
    10921 ggccttgtcc tgcgcgcgca tgtccatcga caacgtcttg gccaccgcgc cgattccgcg
    10981 cggctgctcg gcgccgttga tccagacttc gaaggcgtgc gggtcgccat tgtcgacgtg
    11041 accgacgaaa atcgcgaact ggccgaaggg cgactccacc atgtaggtcc acgccgggtt
    11101 gccggccgcg agccgcggcc ggctcggcca gcgcaggctc gacagcgccg gcgtcggggc
    11161 cgcctccagg cggatgcggc ggtccgcgtc cgaggtatcg aggtcgttcg gcgcttcctc
    11221 gtgcgtggtc accgacagca cgctcccgag cacgctgttg ggacgatagg tggcgatgcc
    11281 cttcaggccg gatttccagg cctcgaaata gaggtccttg aagttttcgt agggatagtc
    11341 ctccggcacg ttcaccgtct tgctgatcgc cgagtccacg aacggcgcca ccgccgccac
    11401 catgcgcatg tggtcgagcg cggagatttc cagcgcggtc acgaactgcg gcggcagctt
    11461 gtcggtgttg ccgcccaggt ggcgatacag gcgccaggcg tggtcctcga cgtcgtagac
    11521 cttctgcgtg ccgtcgtggc tgcgcttctt gcgctggtag gtccaggagt acggcggttc
    11581 gatgccgttg gccgcgttgt cggcgaaggc gagcgagatg gtgccggtcg gcgcgatcga
    11641 cagcaggtgc gaattgcgca ggccgtgcct gcggatcgcc tgcttaagcg cttgcggcag
    11701 ccgcgaggcg aaacgcggcg cggcgagata ctggtccgcg tcgagcaggg gaaaggcgcc
    11761 tttctcctgc gccaggtcca ccgaagcggc gtaagcgtcg tcgcgcatgt gctccgcgat
    11821 ccgtgccgcc atcgcccgcg cttcctcggt gtcgtagcgc agtccgagca tgatcagcgc
    11881 gtctccgagc ccggtgaatc cgagtccgat gcggcgcttg tcgtccgcct cctgcttttg
    11941 ctgaggcagc ggccacacag tggcgtcgag cacgtcgtcg agcatgcgca ccgccacttt
    12001 cgtgactttc gcgaacgact cgaaatcgaa gcgcgccttg ggcgtgaacg ggtgcgatac
    12061 gaacggcgtc aggttgatgc tgccgaggca gcagcagccg taggccggca acatttgttc
    12121 cgcgcaagga ttgctggctt cgatcgtttc gcaataggac agattgtttt cggcgttggc
    12181 ctggtcgatg aagatcacgc cgggctcggc gtggtcgtag gtcgaatcca tgatctgttt
    12241 ccacagatcg cgcgccttga ccgtgcggta cacccacatt ccatcggcgc gctgccgact
    12301 aaccccctct cccttcggga gagggccggg gagagggccg ctgggctgca ccttgtgcac
    12361 cagttcccag tcgccgtcgg cctccaccgt gcgcatgaag gcgtcggtga cgacgacgct
    12421 gacgttgaaa ttgctaaggc tgccgtcgtc cttggcgtgg atgaaacgct cgatatcggg
    12481 atgatcgcag cgcaggatgc ccatctgcgc gcctcggcgg gcaccggccg attccacggt
    12541 ctcgcagctc tggtcgaaca cgcgcatgta ggacaccgga ccgctggcgc tgctctgcgt
    12601 gccgcgcacc aacgcgccgt gcggccggat cgcggagaag tcgtagccca cgccaccgcc
    12661 tcggcgcatg gtctccgctg cctccatgag tgcaacgtaa atgccgggct tgccgtcaac
    12721 ggtttcggac accgaatcgc ccaccggctg gacgaaacag ttgatcagcg tcgccgcgag
    12781 ttgcgtgccg gcggcggagt tgatgcgccc gccgggaata aaaccgcttt ccagcgcttc
    12841 caggaacacc ggctcccatt tggccggctc cttttccaca gcggcaagcg ctcgcgccac
    12901 gcgcaggcgc acgtcgcgca cggacttctc cccgcccttg gcatatttct ccagcaggac
    12961 gtcgatgctg acctgttggg ccggcagcga aagatgggga tcgttggcct tcatgacttt
    13021 atttcctcga attatttcaa tgatacctct gcgccccgca accggcgcgg ttcccgtggt
    13081 ggcatagccg gctccggacg cccgcgctcg cgcaggaagt ccgccagcac tttcgcggtg
    13141 tgcgacgttg cagggtatct cgcaatcgac tcgggcggac ccatcaccac cacgcggcca
    13201 ccaccgtccc cgccctcggg gccgagatcg atcagccagt cggaatcggc catcacgtcg
    13261 agattgtgtt cgatgacgac aacggtattg ccggcatcca ccagccgatg caatacgcga
    13321 atgagcttct ccacgtccgc catgtgcagt ccgacggtgg gttcatcaag gacataaagg
    13381 gtgtgcttgg cgcgaggcgc gaggcgtgag gcgcgaggcg aaaccgaccg cacctcctct
    13441 gcggaaagcg gtagaacgtc agagcgcacc tttgaaagtt cagtcaccaa tttgatccgc
    13501 tgagcctcgc caccggataa cgtcgggctc tgctggccga gggtgagata gcccagtccc
    13561 acgtcctgga gcagacgcag ggcatggtgg atcgacgcgt gcgcggaaaa gaactccacc
    13621 gcttcgtcca cgctcatcat cagcacgtcg ccgatcgact tgcccttgta gcgcaccgcc
    13681 agcgtttcct ggttgaagcg tgcgccgccg cagacctcgc aggtcacttt cacgtccggc
    13741 aggaagctca tctcgatggt cttcaagccc tggccttcgc agccgtcgca gcgtccgccc
    13801 gaagtgttga atgaaaaccg gctcggctcg taaccgcgca tgcgcgcttc ggtggtggcc
    13861 gcgaacagtt tccggatcgc gtcccagaac ccga
//
LOCUS       DASQOP010000341         6150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1787171, whole genome shotgun sequence.
ACCESSION   DASQOP010000341 DASQOP010000000
VERSION     DASQOP010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6150
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1787171"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..5814)
                     /locus_tag="VE030_13305"
     CDS             complement(<1..5814)
                     /locus_tag="VE030_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HZF24430.1"
                     /translation="MKATWSAILLFLAAGTVSAAQVEVFSPQGEVKGVRQVAVRFSEP
                     MVAFGDPRLPEPFDIECPQNGSARWADQKNWVYDFGRDLPAGVRCSFRLKAELLSLAG
                     VRLEGAHAFEFSTGGPAIVETLPYQGVPVDEEQIFILGLDATATDESIAANAYCDIKG
                     ITERVEVRVLAGDDRRVMLEARKDFVHRFVQSLMRGTGMDKLLRSGEVAGLPIAAVQC
                     KRRLPNNAEVRLVWGKGVVSASGVATSQDQTIAYRVRDIFQARFTCDRVSKDAQCLPI
                     MPMRLSFTAPVARTTADKIMLKTAAGKAYPAELPDTRNAAEFVEEVSFTGPFPERTQF
                     MVELPPEVTDDAGRKLANQKRFPLQVRTDEYPPLAKFAARFGIIELKGDGMMPVTLRN
                     LEALVETRMIKTGKQDDPAAEAADRADQAINWLKKKLDESKHDEAGTVPGSYSRVADG
                     DVMAMLEWMKRLRQMEHDRWGYDEKKQERVLEYQVGQASIFTERDRIRRVHVPKPQGA
                     RAFEVVGIPLKKPGFYVVELASPRLGAALLKARNKPYYVQSAALVTDLAVHFKWGSEI
                     SLVWVTTLDTGVPVPKAQVAIQDCSGKVYYRGQTDAQGRARIAAVMPERERLPGCLTD
                     YDRQLVASVRVGDDLSFVMSEWNEGISRWRFNLRGGRFAGPYIATTVFDRTLLRAGET
                     VSMKHFYRRHGAKGFAFVPVDGLPKKAVIQHQGSDEKYEVPLTWDARNTAETVWKIPE
                     GAKKGGYSVLMTDMLDARPGSRGNTRTSGSFRVEEFRVPVTKAEMSGPKTPQVKPASV
                     NLDLSLRYLSGGGAGYAPVKVRALVRPKTVSFRDYDGFTFANGKVVAGIEQQGGYQWF
                     ADEYELEDADAPAPTVPGSGVRPLKSIALDLDATGSARVTLPGLPATDSPQELNAEME
                     YSDPNGEVLTTSTSVALWPSKVILGIKPDSWALSQEQLKFQVIALDLAGKPVKNQQVK
                     VQLYLRNMFTHRKRLVGGFYGYESGAEVKQLDVTCAGKTDERGRLFCAVKPPASGNIL
                     MAAESKDRDGNVAYANGSAWVAGKDAWWFDQSNDDRIDVIPERKRYEPGETAQFQVRM
                     PFQKATALITVEREGVVESFVQPLSGAAPVVKVPIRGNYAPNVFVSVLVVRGRIADLQ
                     PTALVDLGKPAFKMGVAEISVGWRAHELGVNVSAGRQAYKVREKARVTVQVTQRAGGK
                     PVPVKSGEVAIAAVDEGLLELLPNDSWKLLDAMMRARGIEVDTSTASMQVVGKRHYGR
                     KALAQGGGGGRQTARELFDTLLLWKARVPLDDKGRAVVDIPLKDSLTSFRIIAVADSG
                     NGLFGTGETSIRATQDLMLLSGVPPLVREGDRFDARFTVRNASERVMDVEVKGNVEGQ
                     ALPASRMTLAAGEGRDMGWEATVPVNSNTLRWEVSASEMTASGKGAADRLKVLQQVIA
                     AIPVRTFQATLTQLERPLDLAVKIPEDASPERGGVSVALAAKLAGPLPGVLEYMGAYA
                     YTCLEQRISRAIALRDQALWQRTMDALPSFLDSDGFAKYFALERTGSDTLTAYILAIA
                     HESGWSVPEGARGRMQNALTAFVTGRARRDSPLPSADLTIRKVAALEALSRHQAIEPN
                     WLDSIAVEPNLWPTSAVLDWYGVMKRGEALKDRDKRLAEAEQVVRSRLNFQGTTMGFS
                     TERMDYLWWLMVSGDVNANRALLTFLDAEQWREDMPRLARGALGRQHRGHWNTTVANA
                     WGVLAMEKFSAKFESEPVTGQTQARLNEQQGVFDWGKQAQGGVLEFKWPRAEAALSVS
                     QSGTGKPWVTVQSRAAIPLKAALSSGFRVVRTVTPVETKVPGELRRGDVVRVRLELEA
                     QSDMAWVVVVDPVPAGSAILGTGLAKDSRILTSGEQQRGFVRPAFEERTFDAFRAYYR
                     FVPKGSWVVEYSLRLNSIGRFELPPTRVEAMYAPEMFG"
     gene            complement(5865..>6150)
                     /locus_tag="VE030_13310"
     CDS             complement(5865..>6150)
                     /locus_tag="VE030_13310"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HZF24431.1"
                     /translation="VDGDNVSVHKRGVYTHVLTDNFDGHPTTFKESIGTVELDCARRI
                     NRVTRIDMIGESGEVVWSSGPMKKRMWEEVRPNSHAQSTLEVVCARIHKL"
BASE COUNT         1014 a         2123 c         1870 g         1143 t
ORIGIN      
        1 gccgaacatc tccggcgcgt acatcgcctc gacccgcgtc ggcggcagtt cgaagcgacc
       61 gatactgttc aatcgcaagc tgtactcgac cacccaggag cccttcggga cgaagcggta
      121 gtaggcacgg aaggcatcga acgtccgttc ctcgaacgcc ggccggacga agccgcgctg
      181 ctgttcgccg gaggtcagga tccgcgagtc cttcgccagg ccggtgccaa ggatcgccga
      241 accggccggc accgggtcaa ccaccaccac ccaggccatg tcggactgtg cttccagttc
      301 cagccgcacc cgcaccacgt caccgcgccg cagttctccc ggtaccttgg tctccaccgg
      361 cgtgacggtc ctcactactc tgaatccgct ggacagcgcc gctttcaacg gaatcgccgc
      421 gcggctctgc accgtgaccc atggcttgcc ggttccagac tggctgaccg acaacgccgc
      481 ctccgcccgc ggccacttga actccagcac acctccctgc gcctgcttgc cccaatcgaa
      541 tacgccctgt tgctcattga gccgcgcctg ggtctgtccc gtcaccggtt ccgactcgaa
      601 tttcgcggag aatttttcca tcgccagcac gccccaggcg ttggccaccg tagtgttcca
      661 gtggccgcga tgctgccggc cgagcgcgcc gcgggcgagc cgcggcatat cctcccgcca
      721 ctgctcggca tcgaggaagg tgagcagcgc ccggttggcg ttcacgtcgc cggagaccat
      781 cagccaccac aggtagtcca tgcgctcggt ggagaatccc atggtcgtgc cctggaagtt
      841 cagccgcgag cgcacgacct gctcggcttc ggcaagacgc ttgtcgcgat ccttcagcgc
      901 ctcgccgcgc ttcatcaccc cgtaccagtc gagcaccgcc gaagtcggcc acaggttcgg
      961 ctccaccgcg atcgaatcga gccagttcgg ctcgatcgcc tggtggcgcg acaaggcttc
     1021 cagcgccgcc accttgcgga tcgtcaggtc tgcggacggc aacggcgaat cacgcctggc
     1081 gcggccggtg acgaaagcgg tcagcgcatt ctgcatcctg ccgcgcgcgc cctcgggaac
     1141 gctccagccg gattcgtgcg caattgccag gatgtaagcg gtcagcgtgt cgctgccggt
     1201 gcgttccagc gcaaaatatt ttgcgaagcc gtcactatcg aggaaggaag gcaacgcatc
     1261 catggtccgc tgccacaacg cctgatcgcg caacgcgatc gcacgcgata tgcgctgttc
     1321 gaggcaggtg taggcatacg cgcccatgta ttcgagcacg ccgggcagcg ggccggcgag
     1381 cttcgccgcc agcgcaacgc tcacgccgcc gcgttcgggc gacgcgtcct cggggatctt
     1441 caccgcgaga tccaacggcc gctcgagctg ggtgagcgtg gcctggaaag tgcgtacggg
     1501 tatcgcggcg atcacttgct gcaacacctt gagccggtcc gcggccccct tgcccgatgc
     1561 cgtcatttcc gatgcgctga cttcccagcg caaggtattg gaattaacgg gaacggtcgc
     1621 ctcccagccc atgtcgcgcc cttcgccggc ggcaagcgtc atacgcgagg ccggcagcgc
     1681 ctggccttcc acgttaccct tcacttccac gtccatgacg cgctccgagg cgttgcgcac
     1741 ggtgaagcgc gcatcgaaac ggtcgccctc gcgcaccagc ggcggcacgc cggagagcag
     1801 catcaggtcc tgcgtggcac ggatcgaagt ctctccggtg ccgaacaacc cgttaccgga
     1861 atccgcgacc gcgatgatgc ggaacgatgt gagcgaatcc ttgagcggga tgtccaccac
     1921 cgccctgccc ttgtcatcca ggggcacgcg cgccttccac agcagcagcg tgtcgaaaag
     1981 ttcccgggcg gtctggcgcc cgccgccgcc gccttgcgcc agggccttgc ggccgtagtg
     2041 gcgcttgccg accacttgca tggacgccgt cgaggtatcc acctcgatgc cgcgcgcccg
     2101 catcatcgcg tccagcagtt tccaggaatc gttgggcaac agctcgagca ggccttcgtc
     2161 gaccgcggca atcgccactt cgccggactt caccggcacc ggcttgccgc cggcgcgctg
     2221 cgtcacctgc acggtgaccc gcgccttctc gcgtactttg tacgcctgtc tgccggcgct
     2281 gacgttcaca cccagttcgt gcgcccgcca gccgacgctg atctccgcca cacccatctt
     2341 gaaagcaggc ttgccaaggt ccaccagcgc cgtcggctgc aggtccgcaa tgcgcccgcg
     2401 caccaccagc accgaaacga atacattagg tgcgtaattg cccctgatcg gcactttgac
     2461 caccggcgcg gctcccgaga ggggctgcac gaaagattcg accacgccct cgcgctcgac
     2521 cgtgatgagt gcggtggctt tctggaacgg catgcgcacc tgaaactgcg cggtctcgcc
     2581 cggttcgtag cgtttgcgct cgggaatcac gtcgatgcga tcgtcgttgc tctggtcgaa
     2641 ccaccacgcg tccttgcccg caacccaggc cgagccgttg gcgtaagcga cgttgccgtc
     2701 gcggtccttc gattccgccg ccatgaggat gttgccggac gccggcggct ttaccgcgca
     2761 gaacagccgc ccacgctcgt cggtcttgcc ggcgcaggtc acatcgagct gcttcacctc
     2821 ggcgccgctc tcgtatccgt agaagccgcc caccaggcgt tttctgtggg taaacatgtt
     2881 gcgcaggtaa agctgcactt tgacctgctg gttcttgacc ggcttgccgg cgaggtcgag
     2941 cgcgatcacc tggaacttga gctgctcctg cgacagcgcc caggaatccg gcttgatgcc
     3001 gaggatcact ttcgacggcc acagcgccac gctcgtcgac gtggtcagca cttcgccgtt
     3061 cgggtccgaa tattccatct cggcgttcaa ctcttgcggg ctgtcggtgg cgggcagtcc
     3121 cggcagggtc acgcgcgccg agccggtggc gtcaaggtcg agcgcaatgg acttcagcgg
     3181 ccgcacgccg ctgcccggca ccgtcggggc cggcgcgtct gcgtcctcga gttcatattc
     3241 gtcggcaaac cattggtagc cgccctgctg ctcgataccc gcgaccacct tgccgttggc
     3301 gaaggtgaag ccgtcgtagt cgcggaagct caccgtcttc gggcgcacca gggcacgcac
     3361 tttcaccggc gcgtagccgg cgccgccgcc ggagaggtaa cgcaggctca ggtccaggtt
     3421 cacgcttgcc ggttttactt gcggcgtttt cggccccgac atctccgcct tcgtcaccgg
     3481 tacgcggaat tcctcaacgc ggaaactacc cgaagtgcgg gtgttgccgc gcgaacccgg
     3541 ccgtgcgtcg agcatgtcgg tcatcagcac cgagtagccg cccttctttg cgccttcggg
     3601 aattttccac acggtctcgg cggtgttgcg cgcgtcccag gtaagtggca cttcgtattt
     3661 ctcgtccgag ccctggtgct ggatcacggc tttcttcggc aggccgtcca ccggaacgaa
     3721 cgcgaagccc ttggctccat gcctgcgata gaaatgcttc atgctcacgg tctcgccggc
     3781 gcgcagcaac gtgcggtcga ataccgtggt tgcgatatac gggccggcga aacggccgcc
     3841 gcgcaggttg aagcgccaac gcgaaatgcc ctcgttccat tccgacatga cgaacgacag
     3901 gtcatcgccc acgcgcacgg aagccaccag ttgcctgtcg tagtcggtaa ggcagcccgg
     3961 gaggcgttcg cgttcgggca ttacggccgc gatgcgggca cggccctgcg cgtcggtctg
     4021 cccgcggtaa tacaccttgc cgctgcaatc ctggatcgcc acctgcgcct ttggcaccgg
     4081 cacgccggta tccagcgtgg tcacccagac cagcgaaatc tcgctgcccc acttgaaatg
     4141 aaccgcgagg tcggtcacca acgccgccga ctgcacgtag taaggcttgt tgcgcgcttt
     4201 cagcagagct gcacccagcc gcgggctcgc cagttcgacc acgtagaacc cgggtttctt
     4261 cagcggtatg ccgaccactt cgaaggcgcg tgcaccctgc ggtttgggca cgtgcacgcg
     4321 gcgaatgcgg tcgcgctcag taaatatcga cgcctggccc acctggtact cgagcacccg
     4381 ctcctgcttc ttttcgtcgt acccccagcg atcgtgttcc atctgccgaa ggcgtttcat
     4441 ccactccagc attgccatca cgtcgccgtc tgccacgcgc gaatagctgc cgggaacggt
     4501 gccggcttcg tcgtgcttgg actcgtcgag ttttttcttc agccagttga tcgcttgatc
     4561 ggcgcggtcg gcggcctccg cggcagggtc atcctgcttg ccggtcttga tcatgcgggt
     4621 ctcgaccagc gcttccaggt tgcgcagcgt caccggcatc atgccatcgc ccttgagttc
     4681 gatgatgccg aagcgcgccg cgaacttggc cagcggcgga tattcgtcgg tgcgcacctg
     4741 cagcggaaaa cgtttctggt tggcgagttt gcgtccggcg tcgtcggtca cctccggagg
     4801 tagctcgacc atgaactgcg tgcgctcggg gaagggaccg gtgaaactca cttcctcgac
     4861 gaactcggcg gcattacgcg tatccggcaa ctcggccggg taggccttgc cggccgcggt
     4921 cttgagcatg atcttatcgg ccgtggtcct cgcaaccggc gcggtaaacg aaagccgcat
     4981 cggcatgatc ggcaggcact gcgcatcctt gctgactcga tcgcaggtga atcgggcctg
     5041 aaagatatcg cgaacccggt aggcgatggt ctggtcctgg ctggtggcca cgccgctcgc
     5101 ggacacgacg cccttacccc acaccaggcg gacttcggcg ttgttcggca gccgccgctt
     5161 gcactgcacc gccgcgatgg gcaatccggc cacttcacca cttctgagca gtttgtccat
     5221 gcctgtaccg cgcatcagcg actgcacgaa tcgatgcacg aagtccttgc gcgcttcgag
     5281 catcacgcgg cgatcgtcgc cggcgagcac ccgcacttcg acccgctcgg tgatgccttt
     5341 gatgtcgcag taggcgttgg cggcgatgga ctcatcggta gccgtggcgt cgagcccgag
     5401 gatgaagatc tgttcttcgt ccaccggcac cccttgatac ggcagcgttt ccacgatcgc
     5461 gggtccaccg gtggaaaact cgaaggcgtg agcgccttcg agcctgacac cggccagcga
     5521 aaggagctcc gccttcaaac ggaaactgca gcgcacgccg gcgggcagat cgcggccgaa
     5581 gtcgtacacc cagttcttct gatcggccca gcgcgcgctt ccgttctggg gacattcgat
     5641 gtcaaaaggc tccggcagcc gcggatcgcc gaaagcgacc attggttcgg aaaatcgcac
     5701 cgccacctgg cgcacgccct tgacctcacc ctgaggagag aacacttcca cttgcgccgc
     5761 cgagaccgtg ccggcagcga ggaacaacag gatcgcagac caggtcgcct tcattctctc
     5821 cagttcgcgt ccctctgcgt tctttgcggc tttgcggtga agcgtcagag cttgtggatc
     5881 cgcgcgcaga cgacttccag cgtggattgc gcatggctgt tagggcggac ctcttcccac
     5941 atgcgcttct tcatcggacc ggaggaccac accacctctc cgctttcgcc gatcatgtcg
     6001 atgcgcgtga cgcgattgat gcgccgggcg caatccagct cgacggtgcc gatggattcc
     6061 ttgaacgtgg tcggatggcc gtcgaagttg tcggtgagca cgtgggtgta gacgccgcgc
     6121 ttatgcacgc tgacgttgtc gccgtcgacg
//
LOCUS       DASQOP010000342         8875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1186437, whole genome shotgun sequence.
ACCESSION   DASQOP010000342 DASQOP010000000
VERSION     DASQOP010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8875
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186437"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VE030_13315"
     CDS             complement(<1..642)
                     /locus_tag="VE030_13315"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZF24432.1"
                     /translation="MNRHGRFLADRSISVETTYAKPWDRREFVRGMAALAGSAGLLGY
                     DMKPAAAEPPPETTRIRLAQIPGICVAPQYVAGELLRGEGFTDVQYVKVASGAMYKSF
                     GAGDIDISMAFVAPFIMEVDVGNPLVLLAGVHVGCFELFGTDRVHAISDLKGKTVAIP
                     ELGSPHHIFLASMASYVGLNPDRDIKFVTRTPAESIKLLAEKKIDALMGFPPLP"
     gene            complement(1028..2053)
                     /gene="mltB"
                     /locus_tag="VE030_13320"
     CDS             complement(1028..2053)
                     /gene="mltB"
                     /locus_tag="VE030_13320"
                     /inference="COORDINATES: protein motif:HMM:NF024798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HZF24433.1"
                     /translation="MRTAQLLMAIAMVLAGASVPTLAQAPAKVQLKPEVEEFIGQMVR
                     QNQFEYAALRQVFAQLKAHDGVVKAINAPVTAKPWHEFRDIFVSPMRISGGLEFWQQH
                     ADLLARARDVYGVPEEIVVAIIGVETIYGRRLGSFRVIDALYTLGFEMSERATFFRGE
                     MEQFLLLARENSLDPLGVKGSFAGAMGMPQFIPTSYRRFAVDFDGDGKINLWDSAADV
                     IGSVANYLHHFGWVWRQPVTVPARISGTQFKEVLEAGLKPQLTLQQLQPKGVEATEEL
                     PPDLQAGLFSLDLAQGQEYWLSLNNFYVITRYNRSKNYAMAVYQLAKAIASERENATA
                     TQATVDR"
     gene            complement(2058..3266)
                     /gene="ccmI"
                     /locus_tag="VE030_13325"
     CDS             complement(2058..3266)
                     /gene="ccmI"
                     /locus_tag="VE030_13325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HZF24434.1"
                     /translation="MTAFIVSAAVLLLLAVAIVLRPLLKSGAGTGVARDKANFEIYRD
                     QLAELENDLESGVLGAEQFDQAKADLDRRVLEEAARPAEGVTLARSGRAAPIAVGVLI
                     PVAAVLLYLQFGTLEGLQVTQHAAGDVSSMTAERFQEMTEKLAARMREKPDDAEGWMM
                     LGRAYRALERFDEAADALGKAVALYPGNADLLADLAETLALRNGRTLAGEPTKLLERA
                     LKLDPDNDKALALSGSAAFERKDYKAAIRHWEALLKRPKVSGELAQALSAGIAEARAL
                     SAGKKTVAAKPAPGRVSGIVTLDSALRARAAPEDTVFVYARAAQGPRMPLAIVKVQVK
                     DLPYNFSLDDSMAMMPEMKLSAFTEVIVGARVSKSGSAKPAAGDLEGASAKVSPGADG
                     VTVAINQVVR"
     gene            complement(3263..3733)
                     /locus_tag="VE030_13330"
     CDS             complement(3263..3733)
                     /locus_tag="VE030_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012358244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HZF24435.1"
                     /translation="MKRIIAVLLMMLALPAYANEAAPAAQDPALEQRVMRLTSELRCL
                     VCQNQSLADSHADLAIDLKNQVRSQMQAGKSDAEIREFMVARYGDFVLYRPPLKSTTA
                     LLWAGPFLLLAAGGLALGFYLRRRRKRLTESELTPEQRARAEALLHARREGQTR"
     gene            complement(3730..4254)
                     /locus_tag="VE030_13335"
     CDS             complement(3730..4254)
                     /locus_tag="VE030_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HZF24436.1"
                     /translation="MKRFLVPLGIFLVLSVFLAVGLKLDPREVPSPLIDKPAPPFQLA
                     QLRDPGKTLGPGELKGKVWIFNVWASWCVACLQEHPLLVELSRQNLVPIYGLNYKDGR
                     DNALKWLDTHGNPYTLSAMDADGRVGIDYGVYGVPETYVIDKNGVIRFKQIGPVTPKV
                     LEEKILPLVRKLQG"
     gene            complement(4305..6293)
                     /locus_tag="VE030_13340"
     CDS             complement(4305..6293)
                     /locus_tag="VE030_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HZF24437.1"
                     /translation="MIPELGQFALIIALCIALVQGVLPIVGAARGDVAWMNLARPAAQ
                     GQFVFVAIAFGCLAYSFVTNDFSVLNVVNNSNSSLPWYYRFAATWGSHEGSLLLWVLM
                     LTTWMVAVTLFSRHLPLEMVSRILGVMALISVGFLLFMLLTSNPFERLLPAAPDGRDL
                     NPLLQDPGMVIHPPMLYMGYVGFSVAFAFAVAALLGGQLDATWARWSRPWTTMAWVFL
                     TIGIALGSGWAYYELGWGGWWFWDPVENASFMPWLLGTALIHSLAVTEKRGGFKVWTV
                     LLAIMTFSLSLLGTFLVRSGVLTSVHAFATDPARGVFILAFLVVVIGASLTLFAWRAP
                     KVGLGGRFDFVSRESALLANNLFLIVAAASVLLGTLYPLFLDALGLGKISVGPPYFDS
                     VFVPLMAPAIFLMGVGPLAKWKRAELPDLAHRLKWALVVALVTALLLPFTMGKWTPMI
                     AFGLLLALWVAISGVVNLWQRVSQLRTSNGLGTGAQLRGLPRGYYGMLLAHAGVAVFI
                     AGVTLVKGYEVEKDVRMRAGDTVEVGGYTFRLDGFVQVPGPNYEATRGLVSVLKNGKR
                     VGTMFPEKRLYRVQQMPMTEAAIDPGFTRDLYVSLGEPVGNDAWVVRIYHKPFVDWIW
                     GGAFLMALGGVLAITDRRYRLARKEEREVSAAASVVIA"
     gene            complement(6471..6935)
                     /gene="ccmE"
                     /locus_tag="VE030_13345"
     CDS             complement(6471..6935)
                     /gene="ccmE"
                     /locus_tag="VE030_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HZF24438.1"
                     /translation="MKPRHKRMALIAAGLAALGIALALILQAFQSNLVFFFTPTQVAA
                     HEAPRGRNFRIGGLVEQGSVQRQPDGLTVRFVVTDTAKSVAVVYSGILPDLFREGKGV
                     VAQGKLGADGVFSASEVLAKHDQNYMPPEAADAVERAHKAMQESKKTLVEKQ"
     gene            complement(6942..7115)
                     /gene="ccmD"
                     /locus_tag="VE030_13350"
     CDS             complement(6942..7115)
                     /gene="ccmD"
                     /locus_tag="VE030_13350"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HZF24439.1"
                     /translation="MQWGSVSEFFAMGGYALYVWGSYLVTAVLIGLEVATLVRHGRTL
                     RARAGRIAGPESD"
     gene            complement(7311..8042)
                     /locus_tag="VE030_13355"
     CDS             complement(7311..8042)
                     /locus_tag="VE030_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015421710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HZF24440.1"
                     /translation="MAINWFKYSSPQTFYPLAGKMVPWFMVPAAVLGVAGLYIGLFVA
                     PTDFQQGEAYRVIFIHVPAAWMSMFIYVVMAFWAGIGLAFNTRLSSMMAHALAPTGAL
                     MTFIALWTGSLWGRPTWGTWWVWDARLTSELILLFLYIGYMSLAAAIDDPRRADKASA
                     VLALVGVVNIPIIYFSVQWWNTLHQGASVSISRAPSMAAIMLVGMLVMVFAFWAYSIA
                     AALMRVRIMILERERNSAWLRELGR"
     gene            complement(8047..8727)
                     /gene="ccmB"
                     /locus_tag="VE030_13360"
     CDS             complement(8047..8727)
                     /gene="ccmB"
                     /locus_tag="VE030_13360"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HZF24441.1"
                     /translation="MRGSLIAGCHAVVARDLLIALRHRSDVLTTFFFFVIVVSLFPLG
                     VGPEPDTLREIAPGVVWVAALLAAMLSLARMFGADYADGTLEQLVLTPQPLVVLVMAK
                     VTAHWLTTGLPLVLIAPVLGLQFDLPADALATLLLSLLIGTPALSLIGAVGTALTLGV
                     RGAAALTALLVLPLYVPVLIFGAGAVAATATGTGAAGHLSLLGAMLLIALVIAPWATA
                     VALRISLE"
BASE COUNT         1828 a         2918 c         2612 g         1517 t
ORIGIN      
        1 cggaagcggt ggaaagccca tcaatgcgtc aatcttcttc tcggccagga gctttatcga
       61 ttccgccggc gtccgcgtaa cgaatttgat gtcccggtca ggattcaggc ccacataaga
      121 ggccatgctg gcaaggaaaa tatggtgggg cgatcccaac tcgggtattg cgaccgtttt
      181 tcccttgagg tcgctgatgg cgtgcacgcg atcggttccg aacagttcaa agcaaccgac
      241 atgtacgccg gcaaggagca cgagcggatt tcccacgtcc acctccatga tgaacggcgc
      301 gacaaatgcc atgctgatgt cgatgtcacc ggcaccgaac gatttgtaca tcgctccgga
      361 cgcgaccttg acatattgca cgtcagtaaa gccctcgccc cgcaggagtt ccccggcgac
      421 gtactgaggc gcgacacaga taccggggat ctgggccaac cgaatcctcg tggtctcggg
      481 cggcggttct gcggcggccg gcttcatgtc gtagccgagc aacccagcgg acccggccag
      541 tgcggccatg cctctcacga attcccgccg gtcccatggc tttgcgtacg tggtttcgac
      601 ggaaatactt cgatcggcaa gaaaacggcc gtgacgattc ataactgcgt tctcctcagg
      661 gcaaagcacg cacggccaat atacgcagtt gccgatggat gccggcaagc gatgcaacat
      721 gcgaaacgaa cttgttagat ggattggggt attcggggag tgcgctgacg caattcgcgc
      781 gcgcgccttc ctgccaggct gaaaatccct ggccgcaagg tttgccgaga cggatcccgc
      841 gctgacagtg cgtgcggccc atgctgacct cctcccccga ggcgggattg acgggcggtg
      901 tcgctacact actctctgat cacctcggtc gcaacgcgcc gcgccacagt gagtcaagat
      961 tggcccgtag cactcacgcc ggcggacaaa cgccggatga tggccgattg cggtttgcgg
     1021 ccccgtttca gcggtctacg gtggcctgcg tcgcagtcgc gttctcgcgt tcgctggcaa
     1081 ttgccttggc aagctggtac accgccatcg cgtagttctt gctgcggtta taacgggtga
     1141 tgacgtagaa gttattgagt gacagccagt attcctggcc ctgggcgaga tcgagggaga
     1201 agagcccggc ctggagatcg ggcggaagct cctcggttgc ttccacgccc ttcggctgca
     1261 actgctgtaa ggtcagttgc ggcttcagtc cggcttccag gacttccttg aactgcgttc
     1321 cgctgatcct ggccggcacc gtgaccggct ggcgccagac ccagccgaaa tgatgcagat
     1381 agttggccac gctgccgatc acgtcggccg cgctgtccca cagatttatt ttgccatcgc
     1441 cgtcgaaatc caccgcgaac ctgcggtaac tggtgggaat gaactgcggc atccccatcg
     1501 caccggcaaa cgaccccttt actccaagcg gatcgagcga gttttcccga gcgagcaaca
     1561 ggaactgctc catttctccg cggaaaaagg tcgcgcgttc gctcatctcg aagcccaggg
     1621 tgtagagcgc atcgatgacg cggaacgacc cgagccgccg cccatagatg gtctccacgc
     1681 cgatgatagc gacgacgatc tcctcgggca cgccatagac atcgcgagcg cgcgccagca
     1741 ggtcggcgtg ctgctgccag aattcgaggc cgccgctgat gcgcatcggc gagacaaata
     1801 tgtccctgaa ctcatgccag ggtttcgccg tcaccggggc gttgatcgct ttgaccacgc
     1861 cgtcgtgcgc cttgagctgt gcgaatacct ggcgcagtgc cgcgtattcg aactggttct
     1921 gcctcaccat ttggccgatg aactcttcga cctccggctt gagctggacc ttggccggcg
     1981 cctgcgcaag cgtcgggacc gacgcaccgg cgagaaccat cgcaatcgcc atcagcagct
     2041 gcgccgttcg cattgtctta cctgaccacc tggttgattg ccaccgtgac tccgtctgcg
     2101 cccgggctga ccttggcgct cgcgccttcc agatcacccg ccgccggctt ggcgctgccc
     2161 gatttcgaca cgcgcgcgcc gacgatgact tcggtgaacg ccgacaactt catctccggc
     2221 atcatcgcca tcgaatcgtc gagcgaaaaa ttgtacggca gatccttcac ctgcactttt
     2281 acgatcgcca gcggcatgcg cggtccctgc gccgcgcgcg cgtacacgaa cacggtatcc
     2341 tccggtgccg cccgcgcccg caacgcggag tcgagcgtga cgatgccgct gactcgcccc
     2401 ggcgcgggtt tcgccgccac ggtcttcttt cccgccgaca gcgcccttgc ctcggcgatc
     2461 ccggcactga gcgcctgcgc cagctcgccg ctcactttag gacgtttcag caacgcttcc
     2521 cagtggcgga tcgccgcctt gtagtccttg cgctcgaacg cggcgctgcc ggaaagcgcg
     2581 agcgccttgt cgttgtccgg atcgagcttg agcgcgcgct cgagcagctt ggtcggctca
     2641 cccgcgagcg tacgcccgtt cctgagcgcc agcgtttcgg ccaggtccgc gagcagatcc
     2701 gcattgccag ggtaaagcgc caccgccttc ccgagtgcat cggccgcttc atcgaagcgc
     2761 tccagcgccc ggtacgcgcg gccgagcatc atccagcctt cggcgtcgtc cggcttctcg
     2821 cgcatgcgcg ccgccagttt ttccgtcatc tcctggaaac gttcagcggt catggaggac
     2881 acatcgcctg ccgcgtgctg cgtgacctgc aggccctcca gcgtgccaaa ctgcagatac
     2941 agcagcacgg cggccactgg aatcagcacg ccgaccgcaa tcggcgcggc gcgcccgcta
     3001 cgcgcgagcg tcacgccttc ggcgggtctc gccgcttcct cgagcacgcg gcgatcgagg
     3061 tcggctttcg cctggtcgaa ctgttcggcg cccagcacgc cggattcgag atcgttttcc
     3121 agttcggcca actggtcgcg atagatttcg aaattcgctt tatcacgcgc gactcccgtg
     3181 ccggccccgc ttttgagcaa cggccgcagt acgatcgcaa cggcaagcag cagaagaaca
     3241 gcggcgctga cgatgaaggc tgtcatcggg tctgaccctc ccgccgggca tgcagcagcg
     3301 cttcggcgcg ggcacgttgt tccggtgtca gctcactttc ggtaaggcgt ttgcgccggc
     3361 gccgcaggta gaatcccagc gccaggccgc cggcggccag cagcaggaac ggcccggccc
     3421 acagcaacgc cgtggtggat ttgagcggag gcctgtagag gacgaagtcg ccgtagcgcg
     3481 ctaccatgaa ctcgcggatc tcggcatcgc tcttgccggc ctgcatctgg ctgcgcacct
     3541 ggttcttgag atcgatcgca aggtcggcgt gggaatccgc cagcgactgg ttctggcaaa
     3601 ccagacagcg taactccgag gtcagacgca tcacccgttg ctccagtgcc gggtcctgcg
     3661 ccgcgggcgc agcctcattg gcgtaggcag gcagcgccag catcatcagc aggactgcaa
     3721 taatgcgctt catccctgca acttccttac cagcggcagg atcttttcct cgagcacttt
     3781 cggcgttacc ggtccgatct gtttgaagcg gatgaccccg ttcttgtcga tgacgtaggt
     3841 ttccggcacg ccgtaaacgc cgtagtcaat gcccacccgt ccatcggcat ccatcgccga
     3901 cagcgtgtac gggttgccgt gcgtgtccag ccatttcaac gcgttgtcgc gaccgtcctt
     3961 gtaattcaag ccatagatcg gcaccaggtt ctggcgcgac agttccacca gcagcggatg
     4021 ctcctgcagg caagcgacac accaggaggc ccagacgttg aaaatccaca ctttgccctt
     4081 gagctcccct gggccaagcg tcttgccggg atcccgcaac tgggcgagct gaaatggcgg
     4141 cgccggcttg tcgatcagcg gcgaaggcac ttcgcgcggg tccagcttca gaccgacagc
     4201 caggaagacc gaaagaacca gaaagattcc aagggggaca agaaagcgtt tcacttaaaa
     4261 ctggtaaacg gtgaacggtg aatggtgaat ggcagaccaa tcgatcaggc tattacaacg
     4321 ctggcagccg cagacacctc gcgctcctct ttgcgcgcca ggcggtaacg gcggtcggtg
     4381 attgccagaa ctccgcccag tgccatcagg aatgcgccgc cccagatcca gtccacgaac
     4441 ggcttgtggt aaatacgcac cacccaggca tcgttgccga cgggttcgcc gagcgagacg
     4501 tacaggtcac gggtaaagcc ggggtcgatt gccgcctcgg tcatcggcat ctgctgcacg
     4561 cgatacaggc gtttctccgg gaacatggtg cccacgcgct tgccgttctt caggaccgag
     4621 accaggccgc gggtggcctc gtagttcggc ccgggcacct gcacgaagcc gtcgaggcgg
     4681 aacgtgtacc cgccaacttc cacggtgtcc ccggcccgca tgcgcacgtc cttctcgact
     4741 tcgtacccct ttaccagcgt gacgccggca atgaatactg ccactcccgc atgtgccagc
     4801 agcattccat agtaaccgcg cggcaggccc ctcaattgcg cgcccgtgcc gaggccattg
     4861 gaggtgcgta gttgcgacac gcgctgccac agattgacca cgccggagat cgccacccac
     4921 aacgccagca gcagcccgaa cgcaatcatc ggcgtccact tgcccatggt gaagggaaga
     4981 agcaatgcag tgaccagcgc aaccacgagc gcccacttga ggcggtgcgc aagatcgggc
     5041 aattcggcgc gtttccattt tgccagcggg cccacgccca tcaggaagat cgccggcgcc
     5101 atcagcggaa cgaagacgga atcgaagtaa ggcgggccaa ccgaaatctt gcccaatccg
     5161 agggcatcga ggaacagcgg ataaagcgtg cccagcagca ccgacgcggc cgccacgatc
     5221 agaaacaggt tgttggccag cagcgccgac tcgcgcgaaa cgaaatcgaa cctgccgccg
     5281 agaccgactt tgggtgcccg ccaggcaaac aacgtgagcg aggcgccgat caccaccacc
     5341 agaaatgcga ggataaaaac gccgcgtgcc ggatcggtgg cgaacgcgtg caccgaagta
     5401 agcacgcccg aacgcaccag gaaagtgccc agcaatgaca gcgagaaagt catgatcgcc
     5461 agcagcacag tccagacctt gaagccgccg cgcttctcgg tcacggctag cgagtgaatc
     5521 agtgccgttc ccagcagcca cggcatgaag gacgcgttct ccacgggatc ccagaaccac
     5581 cagccgcccc acccgagttc gtagtacgcc cacccgctcc ccaatgcaat gccgatggtg
     5641 aggaacaccc aggccatagt ggtccaggga cgcgaccacc tggcccaggt cgcgtcgagc
     5701 tggccaccga gcaacgcagc gacggcaaac gcgaaagcga ccgaaaagcc gacatagccc
     5761 atgtacaaca tcggcgggtg aatgaccatt ccgggatcct gcagcagcgg attcaggtcg
     5821 cgcccatcgg gcgcggcagg cagcaggcgc tcgaacgggt tcgaggtgag cagcatgaac
     5881 agcaggaagc cgaccgagat gagcgccatc acgccgagaa tgcgcgacac catttcaagc
     5941 ggcagatggc ggctgaacag cgtcaccgcc accatccacg tcgtaagcat cagcacccac
     6001 agcagcaacg agccttcgtg gcttccccag gtcgcggcga agcggtagta ccacggcagg
     6061 ctggagttgg aattgttgac gacgttgaga accgaaaaat cgttggtgac gaacgaatag
     6121 gccaggcatc cgaaagcgat ggcgacgaat acgaactggc cttgcgccgc ggggcgggcc
     6181 aggttcatcc atgcaacatc tccgcgcgcc gcgccgacga tcggcaacac cccctgcacg
     6241 agggcgatgc aaagcgcgat gatgagtgcg aactgtccga gctctggaat catggggcgg
     6301 tgacgggtga cgagtgacgg gtgacgagta ataacaagta acccaattcc agaaatcgag
     6361 tcacgacgtg acagcgcgac aatcgattgg acgactgtac gtaggctttg cgcacaacgc
     6421 tttccgtaga gcggttcatg cgtctcttgt cactcgtcac ttgtcacctg tcactgtttt
     6481 tcgaccagcg tctttttcga ttcctgcatc gccttgtgcg cgcgctcgac tgcgtcggcc
     6541 gcttcgggcg gcatgtagtt ttggtcgtgc ttcgccagca cctccgaagc gctgaacacc
     6601 ccgtccgcgc cgagcttgcc ctgcgcgacc acgcccttgc cctcgcggaa caggtcggga
     6661 aggataccgg aatagaccac cgcaacgctc ttcgcggtgt ccgtgaccac gaagcgcacc
     6721 gtcaagccgt cgggctggcg ctgcacgctg ccctgttcca ccagcccgcc gatgcgaaag
     6781 ttccttcctc gcggggcttc gtgggcggcc acctgcgtgg gcgtgaagaa gaacaccaga
     6841 ttactctgga acgcctggag aatcagcgcc agcgcgattc ccaacgcggc aaggccggcg
     6901 gcgatcaatg ccatgcgctt gtgacggggc ttcatgaacg atcagtcgga ttcgggtcct
     6961 gcaatacgac cggcgcgggc ccgcagagtt cggccatggc ggaccaatgt cgcgacttcg
     7021 agcccgatga ggacggcggt caccagatac gagccccaga cgtacagggc gtatccgccc
     7081 atggcgaaaa attcggaaac actaccccac tgcatgatcc attcctttca aacgacgaac
     7141 tcaccacaga ggcacagaga cacagagaca tagaaaaaaa ggtggaaaaa taattctaga
     7201 ggaaccaagg cacttcacat ctgctcgaac cccttctctt ttccgccatc gtgttggtaa
     7261 ggcgtcatgt ttgctgtcct ccgtgcctct gtgtctctgt ggtaagagcc ttaacgtccc
     7321 agttcgcgca gccaggcgga attgcgttcg cgctccagga tcatgatccg cacccgcatc
     7381 agcgccgccg cgatcgaata ggcccagaac gcgaacacca tcaccagcat gcccaccagc
     7441 atgatggctg ccatgctggg ggcgcggctg atgctcaccg atgcgccctg gtgcagcgtg
     7501 ttccaccact gcaccgaaaa atagatgatc gggatattga ccaccccgac cagcgccagg
     7561 actgccgaag ccttgtcggc ccggcgcgga tcgtcgattg ccgcggccag cgacatgtaa
     7621 ccgatataca ggaacagcag gatcagttct gaagtcagcc tggcatccca aacccaccag
     7681 gtaccccagg tcggtctgcc ccataacgag cccgtccaca gcgcaatgaa agtcatcaat
     7741 gcgccggtgg gcgcgagcgc gtgcgccatc atcgatgaca gccgcgtatt gaacgccagc
     7801 ccgattcctg cccagaacgc catcaccaca tagatgaaca tcgacatcca cgccgccggc
     7861 acatggatga aaatgacccg gtaggcctcg ccctgctgaa aatcggtagg cgcgacgaat
     7921 aaaccgatgt aaagcccggc cactcccaga acggctgccg gcaccatgaa ccacggcacc
     7981 atttttccgg ccagggggta gaaagtctgg ggagacgaat acttgaacca gttgatggcc
     8041 acgtttctat tccagagaga tccttaaagc aaccgcggtc gcccagggcg cgattaccag
     8101 cgcgatcaac agcatcgctc ccagtagcga cagatgccct gccgcgcccg tcccggtcgc
     8161 ggtggcggcc acggcgccgg caccgaagat cagtaccggc acgtaaagcg gcaataccaa
     8221 caacgcggtt agcgccgcgg cgccgcgcac gcccagggtc aacgcggtgc ccaccgcgcc
     8281 gatcagcgac aacgcaggcg ttccgatcag cagcgacaac aacagcgtcg ccaacgcatc
     8341 ggcgggcaga tcgaactgca aacccagcac cggcgcgatc agcaccagcg gcaaaccggt
     8401 cgtcaaccag tgtgccgtaa ctttcgccat caccaggacc accagcggct gcggcgtcag
     8461 caccagttgc tcgagggtgc cgtcggcgta gtcggcaccg aacatgcgcg ccagcgacaa
     8521 catcgcggcc agcagtgccg cgacccagac cacccccggc gcgatctcac gcagcgtgtc
     8581 cggctcgggt cccacgccca gcggaaacag gctgaccacg atgacgaaaa agaaaaatgt
     8641 ggtcaggacg tcgctacgat ggcgcaacgc gatcagcaga tcacgcgcca ccaccgcatg
     8701 gcagccggcg atcagggaac ccctcaatta atgaaccccc agctcgacgg tacgcattgc
     8761 cgtgccggga agctcgactt cctgatgggt ggtaaacatc accatgccgc cgcgctgcaa
     8821 ctgactcgtg atcgttctcg ccagccgctc caccgaggcc gaatccaggg caatg
//
LOCUS       DASQOP010000343         4759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1788933, whole genome shotgun sequence.
ACCESSION   DASQOP010000343 DASQOP010000000
VERSION     DASQOP010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1788933"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(319..825)
                     /locus_tag="VE030_13365"
     CDS             complement(319..825)
                     /locus_tag="VE030_13365"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZF24442.1"
                     /translation="MSRVKLQTSLGDIVLELDAAKAPGSVENFLQYVNDGFYDNTVFH
                     RVINGFMVQGGGFEPGMKQKTTRAPIRNEAESGLKNDAYTVAMARTPNPHSATAQFFI
                     NIADNNFLNFRAATTDGYGYCVFGRVVEGREVVDKIKAVATGSSAMHQDVPKEDVVIT
                     KAEVIEQG"
     gene            complement(822..1394)
                     /locus_tag="VE030_13370"
     CDS             complement(822..1394)
                     /locus_tag="VE030_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013522354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZF24443.1"
                     /translation="MRKLLSAICALLLSSTVAVAANPQVELKTNLGTITVELYPDKAP
                     QTVKNFLEYVQSDFYTGTIFHRVIPGFMIQGGGFSQDFTQRPTRDPVQNEAANGLKND
                     IGTIAMARTANPHSATAQFFINVADNAFLNHTAPTARGYGYTVFGKVVKGMDVVNTIT
                     QLPTGPGGQFAQDVPRQEVLIQGARLVEAK"
     gene            complement(1464..2699)
                     /locus_tag="VE030_13375"
     CDS             complement(1464..2699)
                     /locus_tag="VE030_13375"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HZF24444.1"
                     /translation="MTNALSRRHQAGSRQTLAQALLAIAAAGASYAWAMGLGPPAVAR
                     QLAAPEAMLVRALLDIGESRFSAALEQIDKLLASNPNFRLAQLVKGDLLLARSRPINS
                     IGNASGAPSEQVDGLREEARARLARYQIETPRELAPRYLLQMPQKQKYALVLDSAKST
                     LFLFENIGGAAHYVADYYVTIGKNGLEKLRQGDKRTPIGVYHVVSQLPRDKLSDFYGS
                     GAFPLNYPNEWDRVRGRDGSGIWLHGTPRDTYSRPPRASDGCIVLANADLEALARKLQ
                     IGSTPVVIADALDWARPGEVEALKADLLITMERWRRDWESRDTETYLGHYAVNFSSND
                     MTLVQWSAQKRQVNASKAWIKVQLSDVSLLLYPGKEEIAVATFDQDYASSNLSNRMTK
                     RQYWIKEGGKWRIVYEGAA"
     gene            complement(2700..3671)
                     /locus_tag="VE030_13380"
     CDS             complement(2700..3671)
                     /locus_tag="VE030_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020700663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZF24445.1"
                     /translation="MSRLQTAGLGAALLVLALQCGWAVADNYDDASRLFKQGNHAAAL
                     EKIEAAIAANPRDARARFLKGLILTEQNKPNDAIKVFTALTDDYPELPEPYNNLAVLY
                     ASQGQYDKARKALEMAIRTHPSYAIAHENLGDVYAKMASEAYDKALQLDRGNAAAQTK
                     LAMIKDLFSSSVVPGKGTSQAGVTETTVAAAATPGAKDKKLPPNPPAPGGAETEAVLA
                     AVMGWAKAWSAKDADAYLAYYAPGFQVPGGEPRAAWEATRRDRITKPKSIEVTVGSPK
                     VSFDANGRAIVSFRQGYKSDTLNTAGAKTLTLTKISDRWLIIQERMN"
     gene            3821..>4759
                     /gene="cysS"
                     /locus_tag="VE030_13385"
     CDS             3821..>4759
                     /gene="cysS"
                     /locus_tag="VE030_13385"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HZF24446.1"
                     /translation="MLKIYNSLTRAKETFTPIFAGKVGMYVCGMTVYDYCHLGHARVM
                     VVFDVVKRWLVAGGFDTTYVRNITDIDDKIIKRAAENNEPIEKLTARFIEAMNQDAAA
                     LGVQKPDIEPRATANVPQMIQLIAALIGKRLAYQAQNGDVYYSVHGFPGYGKLSGKTL
                     DELRAGERVQVDPSKRDPLDFVLWKSAKPGEPAWDSPWGKGRPGWHIECSAMSERYLG
                     SHFDIHGGGQDLQFPHHENEIAQSEGAHGHAFVNYWMHNGFVRVDNEKMSKSLGNFFT
                     VREILTKYDAEVVRFFIVRAHYRSPLNYSDQHLDDAR"
BASE COUNT          848 a         1457 c         1462 g          992 t
ORIGIN      
        1 cccgtcgtcg ccgatccaat actcgaacag gtcgccgagc acgtacaagg cctcggccgc
       61 aggcgcggag tcgcgcagaa agcgcatcag cgcctcggtc gccgccgacc ggtccggaga
      121 cagatgcaga tcggagatga agagcgtatg tggcactgaa ggcaaggatg aaggatgaag
      181 gcggaaggat gaagaagggc tccgacggca accacaaagt taacggcagg ctaccaaggg
      241 cagcctgtct gaatccggtt ctcttgatgt cttggtgccg cagtcgttgg ttttacttca
      301 tccttcatcc ttctgccttc atccttgctc tatgacttcg gccttcgtga tgacgacgtc
      361 ttctttcggg acgtcctggt gcatcgcgct gctgccggtg gccacagcct tgatcttgtc
      421 gacgacctcg cggccctcga ccacgcgccc gaacacgcag tagccgtaac cgtccgtggt
      481 ggcggcgcgg aagttgagaa aattgttgtc ggcgatattg atgaagaatt gcgcggtggc
      541 cgagtgcgga ttgggtgtgc gcgccatcgc aacggtgtag gcgtcgttct tgaggccgct
      601 ttcggcctcg ttccttattg gcgctctggt ggtcttctgc ttcatgccgg gctcgaatcc
      661 gccgccctgg accatgaagc cgttgatcac gcgatgaaag acggtgttgt cgtaaaaccc
      721 gtcgttgacg tactgcagga agttctccac gctgccgggc gcctttgcgg catcgagctc
      781 gaggacgatg tcacccagcg aggtttgcag tttcacgcgg ctcatttcgc ctccaccagc
      841 ctggcgcctt ggatcagcac ttcctgccgc ggcacatcct gcgcaaactg gccgcccgga
      901 ccggtcggaa gctgggtgat cgtgttgacc acgtccatcc ccttcaccac tttgccgaat
      961 accgtgtagc cgtatccgcg cgcggtcgga gcagtatggt tcaggaacgc gttgtcggcg
     1021 acgttgatga agaactgcgc ggtggccgag tgcggattcg ccgttcgtgc catcgcgatg
     1081 gttccgatat cgtttttcag cccgttggca gcttcgttct ggaccggatc gcgagtgggc
     1141 ctctgggtaa agtcctgcga gaagccgccg ccctggatca tgaatccggg aataacgcga
     1201 tggaatatcg tgcccgtgta gaaatcgctt tgcacgtact cgaggaaatt cttcaccgtc
     1261 tgcggtgcct tgtccggata aagctcgacg gtgatggtgc cgagattggt tttcagctcc
     1321 acctgcgggt tggcggcgac tgcaaccgtg ctgctcaaca aaagagcgca gatcgcgctc
     1381 agtaatttcc tcataatgat gtccttgcga tttgattttc gacgcggcac tcagcacgca
     1441 gtacccagta cttagcgcgg actctacgcc gccccttcgt atacgatgcg ccacttgccg
     1501 ccttccttga tccagtattg gcgcttcgtc atcctgttgg agaggttgct gctggcgtaa
     1561 tcctggtcga aggtggctac cgcaatttct tccttgcccg gatagagcaa cagtgacaca
     1621 tccgacaact gcaccttgat ccaggccttg gaagcgttca cctggcgctt ttgcgccgac
     1681 cactgcacca gcgtcatgtc gttggaagaa aagttcacgg cgtaatgccc caggtaggtt
     1741 tcggtgtcgc ggctttccca gtcgcgccgc cagcgttcca tggtgatcaa cagatcggct
     1801 ttcaatgcct cgacttcacc gggcctggcc caatcgagcg catcggcgat caccaccgga
     1861 gtggacccga tctgcagttt gcgggcaagc gcctccagat cggcgttggc gagcacaatg
     1921 cagccgtcgc tggcgcgcgg aggccggctg taggtgtcgc gcggggttcc gtgcagccag
     1981 atgccggaac cgtcacggcc gcgcacgcgg tcccactcgt tcggatagtt gagggggaat
     2041 gcgccgctgc cgtagaaatc gctgagttta tccctgggca actggctgac cacgtggtaa
     2101 acgccgatcg gcgtgcgctt gtcgccctgg cgcagctttt ccaatccgtt tttgccgatg
     2161 gtcacgtagt agtcggccac gtaatgcgct gcgccgccga tattctcaaa caggaacagc
     2221 gtcgatttgg ccgagtcgag gaccagcgcg tacttctgtt tctgcggcat ttgcagcagg
     2281 tatcgcggag cgagctcgcg cggagtctcg atctggtagc gcgccagtcg cgcgcgcgcc
     2341 tcctcgcgca aaccgtcgac ttgctcggaa ggcgcgccgg aagcgttgcc gatgctgttg
     2401 atcgggcggg aacgcgcgag cagcaggtct cctttcacca gctgcgccag gcggaaattg
     2461 ggattcgacg cgagcagctt atcgatctgt tcgagcgcgg cagagaatct gctctcaccg
     2521 atgtccagca gtgcccgcac cagcatggct tcgggcgcgg cgagctggcg tgccaccgcc
     2581 ggcgggccca gtcccattgc ccacgcatac gatgcgcctg ccgcggcgat tgccagcagc
     2641 gcctgcgcca gcgtctgcct cgaacctgcc tgatgtcggc gtgatagggc gttggtcacc
     2701 tagttcattc tttcctggat gatcagccag cggtcgctga tcttggtgag cgtcagcgtc
     2761 ttggcgccgg cggtgttcag cgtgtcggac ttgtatcctt ggcgaaaact cacgatggcc
     2821 cggccattcg catcgaagga aaccttcggc gagcccaccg tgacttcgat cgacttcggc
     2881 ttggtgatac gatcgcgccg ggtggcttcc caggccgcgc gcggctcgcc cccgggtacc
     2941 tggaagccgg gcgcgtagta ggcgaggtac gcgtccgcat cctttgccga ccaggccttg
     3001 gcccagccca tcaccgcagc cagcaccgcc tcggtttccg ccccgcccgg tgcaggcggg
     3061 tttggcggca gcttcttgtc ttttgcgccg ggtgttgccg cggccgcaac cgtagtttcg
     3121 gttacacccg cctgcgacgt ccccttgccc ggcaccacgc tgctcgagaa cagatccttg
     3181 atcatcgcca acttggtttg cgccgcggcg ttgccacggt ccagctgcaa cgccttgtca
     3241 taggcttcgc tcgccatctt ggcgtacacg tcgccgaggt tctcgtgcgc gatggcgtaa
     3301 ctcggatggg tacggatcgc catctcgagc gccttgcgcg ccttatcgta ctgcccctgc
     3361 gaggcataca ggacggcgag attgttgtag ggttcgggca actccgggta atcgtcggtc
     3421 agggccgtga acaccttgat cgcgtcgtta ggtttgttct gctcggtgag gatcagtccc
     3481 ttgagaaagc gtgcgcgcgc gtcgcgcgga ttggcagcaa tcgcagcctc gatcttttcc
     3541 agcgcggcgg catggttgcc ttgcttgaac agacggctgg catcgtcgta gttgtcggcg
     3601 accgcccatc cgcactgcag tgcaagtacg agcagtgcgg cgcccagccc tgcggtttgc
     3661 aatcggctca tgtgctaaac tttacctcaa taagaaacgg taggttattg taaataatag
     3721 tagattctat cagcagagcg ccctgttcga caatccgagg gcgctcaggc ccagcggccc
     3781 cgtttggcgg cgccgtgcac ccgacccaga atgattcccg atgctgaaga tttataactc
     3841 gctcacccgc gccaaggaga cttttacccc gattttcgcc ggaaaggtgg gaatgtacgt
     3901 gtgcgggatg accgtctacg actactgcca tcttggccat gcgcgggtga tggtggtgtt
     3961 cgacgtggtc aagcgctggc tggtcgccgg cggcttcgac accacttatg tgcgcaacat
     4021 caccgacatc gacgacaaga tcatcaagcg cgccgccgag aacaacgaac ccatcgaaaa
     4081 gttgaccgcg cgtttcatcg aggcgatgaa ccaggatgcg gctgcgctcg gcgtgcagaa
     4141 gccagatatc gaaccgcgcg ccaccgccaa cgtcccgcag atgattcagc ttatcgccgc
     4201 gctgatcggc aagcgcctgg cctaccaggc gcagaacggc gacgtctatt actcggtgca
     4261 cgggttcccc ggctacggca agttgtccgg caagacgctg gacgaactgc gcgccggcga
     4321 gcgcgtgcaa gtcgatccca gcaagcgcga tccgctggac ttcgtgctgt ggaaatcggc
     4381 gaagccgggc gagccggcct gggattcgcc ctggggcaaa gggcggcccg gatggcacat
     4441 cgagtgctcg gcgatgagcg aacgctacct cggcagccac ttcgatatcc acggcggcgg
     4501 ccaggacctg cagtttccgc accacgagaa cgagatcgcc cagtcggaag gcgcgcacgg
     4561 ccacgccttc gtgaactact ggatgcacaa cggcttcgtg cgcgtggaca acgagaagat
     4621 gtcgaagtcg ctcggtaatt ttttcaccgt gcgcgaaatc cttacgaagt acgacgcaga
     4681 agtggtgcgc ttcttcatcg tgcgtgcgca ctatcgcagc ccgctgaatt actccgacca
     4741 gcacctcgat gacgccagg
//
LOCUS       DASQOP010000344         6117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1789709, whole genome shotgun sequence.
ACCESSION   DASQOP010000344 DASQOP010000000
VERSION     DASQOP010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6117
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1789709"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1392)
                     /gene="ntrC"
                     /locus_tag="VE030_13390"
     CDS             complement(<1..1392)
                     /gene="ntrC"
                     /locus_tag="VE030_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HZF24447.1"
                     /translation="MKPVWIIDDDRSIRWVFEKALARENIAFKVFASAQEALQALSAS
                     TPQVVVSDIRMPGGSGLELLQQLKEKHPNLPVIIMTAYSDLESAVAAFQGGAFEYLPK
                     PFDVDQAVDLIRRAMEESLRQGDVAEVAEESPEILGQAQSMQEVFRAIGRLTQSHAIV
                     LINGESGTGKELVARALHRHSPRAPKPFIAINTAAIPKDLLESELFGHERGAFTGAQA
                     MRRGRFEQAEGGTLFLDEIGDMPPDLQTRLLRVLSDGQFYRVGGHQPIKANVRIIAAT
                     HQDLESRVKQGLFREDLFHRLNVIRLRLPALRERREDIPLLARYFMQKSARELSVETK
                     KLSETAMKYLSSLDFPGNVRQLENLCHWLTVMAPGMNVDVKDLPPELREEALVPSAEN
                     WVTALEREAEALLKRGDSAILDDLSRRFERALITRALAHTGGRRIEASVLLGMGRNTL
                     TRKIQELGLEKDDG"
     gene            complement(1590..2681)
                     /gene="glnL"
                     /locus_tag="VE030_13395"
     CDS             complement(1590..2681)
                     /gene="glnL"
                     /locus_tag="VE030_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HZF24448.1"
                     /translation="MARFLLDHTRSVCGAAMMQLSLHGIDALATAVVLLDDAQVVRYI
                     NPAAENLLAISAKNIVGTPLEKVFAEPGLLVAAVNYAAENNCSHTQHELPLSTAGRAR
                     LEVSCTVTPVGIARVEGFLIELSPLHQQLRIAREERLMDQSTQTRELIRNLAHEIKNP
                     LGALRGAAQLLDRELERPALHEYTQVIMKEADRLQSLMDRLLTPHRLPQVTQFNVHEA
                     LERVRSLLLAEFPDGIGIRRDYDVSLPPLTADREQIIQSLLNVARNAAQAMRGKGTII
                     LRSRVARRATLAKRMYRLALLVQIVDDGPGVPEELKDRIFFPLVSGREGGHGVGLTIA
                     QTFVTQHHGTIELESEPGRTCFSILLPIE"
     gene            complement(2752..3225)
                     /locus_tag="VE030_13400"
     CDS             complement(2752..3225)
                     /locus_tag="VE030_13400"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HZF24449.1"
                     /translation="MRLCHWALLLAIASIALPARAETCKYVDGEGRVIYSNTPNNPPK
                     GAKLVKCFSDPAPTPAPQESKPESGAREGFPKVDGQTQKKRDDARRGILEKELADEQQ
                     QLEAARQKLAEEEAIRTGNERNYQRYLDRVQPFRDAVANHERNIEAIKREISNLK"
     gene            complement(3402..4811)
                     /gene="glnA"
                     /locus_tag="VE030_13405"
     CDS             complement(3402..4811)
                     /gene="glnA"
                     /locus_tag="VE030_13405"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010395217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HZF24450.1"
                     /translation="MAAADVFKLLKDNDVKFVDLRFTDTRGKEQHVTVPVRAFGPEKF
                     EDGHAFDGSSIAGWKGIQASDMLLMPDPDSARLDPFSDEPTLNITCDVIEPADGKGYD
                     RDPRTIAKRAEAYLKSTGIGDTAYFGPEPEFFIFDGVTWSIDMSGCFVKISSEEAPWS
                     TGKEFEGGNMAHRPPVKGGYFPVPPVDSLQDIRNAMCLALEQQGVEVEVHHHEVAAAG
                     QNEIGTKFNSLVKRADWMQILKYTVWNVAHSYGKTATFMPKPIVGDNGSGMHVHQSVW
                     KGGQNLFAGNGYAGLSELALYYIGGIIKHAKALNAITNPGTNSYKRLVPGFEAPINLA
                     YSAKNRSAACRIPYVASPKARRVEVRFPDPTANPYLAFAAMLMAGLDGVQKKIHPGDP
                     IDKNLYDLPPEEVKKVPHPCASLDEALENLDKNREFLTKGGVFSNDMIDAYIDLKMEE
                     VTRFRMTTHPVEFDMYYSA"
     gene            5118..5570
                     /locus_tag="VE030_13410"
     CDS             5118..5570
                     /locus_tag="VE030_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZF24451.1"
                     /translation="MTTISDILNKAGERGREMNLPYGGALLPQEAYELMQKAPGAKLV
                     DVRTRAEWDYVGRIPGSVQIEWQTYPGSKPNPNFLNELQSQTDTQAVVMFLCRSGARS
                     HAAAAVASQAGYSRSYNVLQGFEGDKDASGHRNSVGGWRVAGLPWVQS"
     gene            complement(5664..>6117)
                     /locus_tag="VE030_13415"
     CDS             complement(5664..>6117)
                     /locus_tag="VE030_13415"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HZF24452.1"
                     /translation="IILWAERRKHVIRIQNVEDMRWTDALKVGIAQTFALIPGTSRAG
                     STIIGGLFFGLSRKAATEFSFFLAIPVLFAASAYELVRNRQLFFAEDLGIFGIGLAAA
                     FVSAFLCVRWLLRYISRHDFTVFAWYRIGFGILVLLTAYTGAVSWSVN"
BASE COUNT         1151 a         1819 c         1892 g         1255 t
ORIGIN      
        1 cccgtcgtcc ttttccaggc ccagttcctg gatcttgcgg gtcagcgtgt tgcggcccat
       61 ccccagcagc accgaggctt cgatcctgcg gccgccggtg tgggcaagcg ccctggttat
      121 gagcgcgcgc tcgaaccggc gggaaaggtc gtcgaggatc gcgctgtcgc cgcgtttcag
      181 cagcgcttcc gcttcccgct ccagcgccgt cacccagttc tccgccgagg gaacaagggc
      241 ttcctcgcgc aactccggag gcaggtcctt gacgtcgacg ttcattcccg gcgccatcac
      301 tgtcagccaa tggcaaaggt tctccagctg gcgcacgttg ccggggaagt ccagcgagga
      361 cagatatttc atcgccgtct cggacagttt cttggtttcc acgctcaatt cacgcgcgct
      421 tttctgcata aagtatctgg ctagcaacgg aatgtcctcg cggcgctcgc gcagcgccgg
      481 caggcgcaac cggatcacat tcaatcgatg gaataaatcc tcccggaaca ggccctgctt
      541 gacccgggat tcgaggtcct ggtgcgttgc cgcgatgatg cgcacgttgg ccttgatcgg
      601 ctgatgcccg ccgacgcggt agaactgacc gtccgacagc acccgcagca ggcgggtctg
      661 caggtcgggc ggcatgtcgc cgatctcgtc cagaaacaaa gtgccgcctt cggcctgttc
      721 aaaacgaccg cgccgcatgg cctgcgcgcc cgtgaaggcg ccccgttcgt gcccgaacaa
      781 ctccgattcg agcagatcct tgggaattgc cgcggtgttg atggcgatga atggtttggg
      841 cgcgcgtggg ctgtggcgat gcagcgcgcg tgccaccagt tccttgccgg tgccggattc
      901 gccattgatg aggacgatgg cgtgcgattg ggtgagccgc ccgatggcgc ggaacacttc
      961 ctgcatggac tgcgcctggc caaggatctc gggtgattcc tccgccactt cggcgacgtc
     1021 tccctgacgc aggctctctt ccatcgctcg gcggatcaga tccacggcct ggtctacgtc
     1081 gaagggtttg ggcaggtatt cgaatgcgcc accctggaat gcggccaccg cgctctcgag
     1141 atccgaatac gcggtcatga tgatgaccgg caggttgggg tgcttctcct tgagttgctg
     1201 cagcaattcg aggccggagc cgcccggcat gcgaatgtcg ctgaccacca cttggggcgt
     1261 cgatgccgac agcgcctgca gggcttcctg cgccgaggcg aaaaccttga aggcaatgtt
     1321 ctcccgcgcg agcgccttct cgaacaccca gcgaatcgag cggtcgtcgt cgatgatcca
     1381 gacaggtttc atggttctaa atgagcgatt gagtgattga gtgattgggt gattgagtga
     1441 ttgggtaatt aagtgattgt gtaccgaatc acctaatccc ttaatcactg aatccctgat
     1501 tgggcaattg agcgatgagt gattgagtca ctgagtccct taatccctca atcgcttaat
     1561 cactgaatcg cccattcccc attccccgtt tattcaattg gcagcaggat gctgaagcag
     1621 gtcctgcctg gttcgctttc cagttcgatg gtgccgtggt gctgggtcac gaaggtctgg
     1681 gcgatggtca gtccgacacc atggccgccc tcgcgcccgc tcaccagtgg aaagaagatc
     1741 ctgtccttca gttcctccgg cacacccggc ccgtcgtcca cgatctgcac cagcagcgcc
     1801 aggcgataca tcctctttgc cagtgtcgcc cggcgtgcca cgcgcgagcg aaggatgatc
     1861 gtgcctttcc cgcgcatggc ctgtgcggca ttgcgggcca cgttgagcag cgactggatg
     1921 atctgctcgc gatccgcagt caatggcggc aggcttacat cgtagtcgcg ccggatcccg
     1981 attccgtcgg gaaactccgc cagcagcaga ctgcgcacgc gctccagcgc ctcgtgcacg
     2041 ttgaactgcg tgacctgtgg cagccggtgc ggcgttagca gcctgtccat cagcgactgc
     2101 agccggtccg cctccttcat gatcacctgc gtgtactcat gcagggcggg ccgttccagt
     2161 tcccggtcga gaagttgtgc cgcgccgcgc agcgccccca gagggttctt gatctcgtgc
     2221 gcaagattgc ggataagctc gcgggtctgc gtactctggt ccatcaggcg ctcctcgcgc
     2281 gcgatgcgca gttgctggtg cagcgggctc aactcgatca ggaacccctc gacccgggca
     2341 atccccaccg gcgtcactgt gcaagacact tcgagccggg cgcgccctgc cgtggacagc
     2401 ggcaattcat gctgcgtgtg gctgcagttg ttctcggcgg cgtagttcac cgcggcaacc
     2461 agaagaccgg gttcggcaaa caccttctcg agcggggtgc cgacgatatt cttggcgctg
     2521 atcgcaagca ggttctctgc cgccgggttg atgtaacgca cgacctgtgc gtcgtccagc
     2581 agaacaaccg cggtcgccag cgcgtcgatt ccgtggagac tcagttgcat catcgcagcg
     2641 ccacacaccg aacgggtgtg atcgagcaag aagcgcgcca cggggattgc gccgaaaggc
     2701 ggatcgcgac acgcggtcgg caaaggctga tgtggctcga cggcgcgccg cttatttcag
     2761 gttcgagatc tcgcgcttga tcgcttcgat attgcgttcg tggttcgcaa cggcgtcgcg
     2821 aaaaggctgg acgcggtcga gataacgctg atagttgcgc tcgttccctg tccgtatcgc
     2881 ttcctcttcg gcgagtttct gcctggcggc ctcgagttgc tgctgctcgt cggcgagctc
     2941 cttttcgaga atgccccggc gcgcgtcgtc gcgcttcttc tgggtctggc catccacctt
     3001 ggggaaccct tcgcgggcgc cggactccgg tttggattcc tgcggcgccg gcgttggcgc
     3061 agggtcgctg aagcatttca ccaatttcgc gcccttggga ggattgttcg gcgtgttcga
     3121 atagatcacg cgaccctcgc catccacgta cttgcaggtc tctgcccgag caggtagcgc
     3181 gatcgacgcg attgccagca acagtgccca atgacaaagc cgcatgaaat tcacccgtga
     3241 gatcaccgaa aaaatgaggc gcgcctgtca cgcgatgcgc tccccgccgg agtctaagac
     3301 aggcattttc tattatcaat ccgcgagtcc catcagagag ttgatctggc acaaacgaaa
     3361 aagggcggga acacccgccc tttctcccgt cgcgatggcg gttacgcgct gtagtacatg
     3421 tcgaattcga ccggatgggt ggtcatgcgg aagcgcgtga cttcctccat cttgaggtcg
     3481 atgtaagcgt cgatcatgtc gttggaaaac acaccgccct tggtgagaaa ctcgcgattc
     3541 ttgtcgaggt tctccagcgc ctcgtcgagc gacgcgcacg ggtgcggcac cttcttgact
     3601 tcttccggcg gcaggtcgta caggttcttg tcgatcgggt cgccgggatg gatctttttc
     3661 tggacgccgt ccaggccggc catcagcatg gccgcgaaag cgaggtaagg gttcgcggtc
     3721 gggtccggga agcgtacctc cacgcgccgc gccttcgggc tggcgacgta aggaatgcgg
     3781 caggccgccg agcgattctt cgcggagtag gcgagattga tgggcgcctc gaatccggga
     3841 accaggcgct tgtaggagtt ggttccgggg ttggtgatgg cgttgagcgc cttggcgtgc
     3901 ttgatgatgc cgccgatgta ataaagcgcc aattcggaca agccggcgta gccgttgccg
     3961 gcaaagaggt tctgtccgcc tttccacacc gactggtgca cgtgcatgcc ggagccgttg
     4021 tcgccaacga tcggcttggg catgaaggtg gcggtcttgc cgtaggaatg ggcgacgttc
     4081 cagacggtat atttcagaat ctgcatccag tccgcgcgct tcaccaggct gttgaacttg
     4141 gtgccgatct cgttctggcc ggcggccgcc acctcgtggt gatgcacctc cacttccact
     4201 ccctgctgct cgagcgccag acacatcgcg ttgcggatgt cctgcagcga gtcaaccggc
     4261 ggcacaggga aatagccgcc cttgaccggg gggcggtgag ccatgttgcc gccttcgaac
     4321 tccttgccgg tggaccaggg cgcttcctcg gaggagatct tgacgaagca gccggacatg
     4381 tcgatgctcc aggtcacgcc gtcgaatatg aagaactcgg gctcgggtcc gaaataagcc
     4441 gtgtcgccga tcccggtgga tttcagatag gcctcggcgc gtttggcgat ggtacgaggg
     4501 tcgcggtcat agcccttgcc gtcggcgggt tcgataacgt cgcaggtaat gttcagcgtg
     4561 ggttcgtcgg aaaacgggtc aaggcgcgcg gagtcggggt ccggcatgag cagcatgtcc
     4621 gaggcctgga tgcccttcca cccggcaatc gaggagccgt caaacgcatg gccgtcctcg
     4681 aacttttccg ggccaaaggc gcggacaggg acggtaacgt gttgttcctt gccgcgggta
     4741 tcggtaaacc gcaggtccac gaacttcacg tcgttgtctt tgagcaattt gaaaacatcg
     4801 gctgccgcca ttttcttctc ctggtttggg gtggtttgca cgggaaatcg agcgagtcac
     4861 acgttcagca ggaaccgtgc cgaccaaaat ctcccgaaaa atcattaatt cacaacttgt
     4921 taaccgaatt ctcccgcaga cctccgcacc aatggagagc gggaagggga ttcgcgctcg
     4981 aaaatagtgc acaacactgc acttggcaag agcttttttg gcgaccccgg actttctgtt
     5041 agcctagtgc gcagaggctt caggaggccc gcgaaaaggc tatggagctg ggttaagtat
     5101 cgattggaga aacaattgtg accactattt cagatatttt gaacaaagct ggcgagcgcg
     5161 gcagggagat gaatctgcct tatgggggtg cattgcttcc gcaggaggcc tacgaactga
     5221 tgcagaaagc gccgggagcg aaactggtag acgtgcgtac ccgggccgag tgggactatg
     5281 tcggccggat tccgggcagc gtccagatcg aatggcaaac ctatcccgga tccaaaccaa
     5341 atccgaattt cctcaacgag ctccagtcac agacggatac gcaggcggtc gtgatgttcc
     5401 tgtgccgcag cggcgcgcga tcgcatgccg ctgcggcagt cgcatcccag gcaggatatt
     5461 cgcgcagcta caacgtattg caggggttcg agggcgataa ggacgccagc ggtcaccgca
     5521 actccgtcgg cggctggcgc gtggcgggac tgccctgggt gcaaagctga gaagcggtga
     5581 ttgagtgatt gagtgattga gtaattgggt gatcgggtta ccgaatcact taatcgctta
     5641 atcacctaat cactgaatca ctgtcagtta acgctccagc tcaccgcgcc ggtatacgcg
     5701 gtcaggagca ccagaatgcc gaagccgatc cggtaccacg cgaacacggt gaaatcgtgc
     5761 cggctgatgt agcgcaacag ccagcgtacg cacaggaatg ccgaaacaaa tgccgccgcc
     5821 aatccgatgc cgaaaatccc gagatcctct gcgaagaaaa gttgccggtt cctgaccagt
     5881 tcataggccg acgcggcgaa caggaccggg atggcgagaa aaaaggagaa ctcggtcgcg
     5941 gccttgcgcg acagtccgaa aaacagtccg ccgataatgg tcgagccggc gcgggacgtt
     6001 cccggtatca gcgcaaaagt ctgtgcaatg ccgaccttga gcgcgtcggt ccagcgcatg
     6061 tcctcgacat tctggatcct gatgacatgt ttgcgccgtt cggcccacag gatgatc
//
LOCUS       DASQOP010000345         4078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2398602, whole genome shotgun sequence.
ACCESSION   DASQOP010000345 DASQOP010000000
VERSION     DASQOP010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2398602"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VE030_13420"
     CDS             complement(<1..233)
                     /locus_tag="VE030_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24453.1"
                     /translation="MTFMVPTVLDETGFDRFVTDNDTAVIGFFGEDSDAATFAALAND
                     VLGKHPAVAFAQVYRNSRGLFDMFGLHGTATAI"
     gene            complement(248..370)
                     /locus_tag="VE030_13425"
                     /pseudo
     CDS             complement(248..370)
                     /locus_tag="VE030_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
     gene            complement(436..3549)
                     /locus_tag="VE030_13430"
     CDS             complement(436..3549)
                     /locus_tag="VE030_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZF24454.1"
                     /translation="MISFIVRQSIRYPGVIVALALTIVAYGLFTLSRAHLDVFPEFSP
                     AQVVIQTEAPGLSAELVETQVSQSIENALSGASGLESLRSQSIPGLSVVHVFFRDGSD
                     VYLNRQVVGERLASLAAQLPKGVVPTMTPLTSSASTVLGIGVTSERRSLMDLRTLVDW
                     SIRPHLLAVRGVAEVNVFGGDVLQWQIRIDPGRLQRYGLGLQDAVAAARTAAGVAGTG
                     FIKTPNQHIMLQTDAQPDALSDVARTILAYRDGRAIRIGDVAQVAEGAAPSIGAAAID
                     GTPGVFLMIQGQLGANTHAVTQDLEAALAELKPLLDAERVRLHPNLFRPANFIETAVR
                     NVQRDILIGSTLVIVVLFLFLFNSRTAFICATAIPVSLLTAVLVLDYFGIGLNIMVMG
                     GLAIAVGEVVDDAIIDTENIFRRLRLNRTASKPRPVWEVVLGASVEVRSSVVYATFIV
                     ALVFVPLLTLGGVAGKLFAPLGLAYILSILASLVVAVTLTPALCYLLLARVDLKSDDP
                     PFVKWLKPRYLGVLRRIERYPGRVIGVTGVVIAIGIGMLPLFSGEFIPALREGHYIMH
                     MSAVPGTSEAESLRIGQKVSQAIGSIEGVRSVAQWVGRSKNGADTFGTHYSEFEIEIG
                     ALAGEDQARILREIRATLSGERSGAFPGVTFAVNTFLTERIEETITGYAAPFVVNLYG
                     SSLDSLDRDALAIATTLASVPGAHDVQIHAPPGTPQLSVRLHHERVAAMGLQPLTVLE
                     AVQAAYEGARVAQIYRGSRVVDLVVLLDPQHRNRINEVGALPLRNLDGRLIRLADVAT
                     IEMTGGRYKVLHSAGRRIQTVTANVVARDIAKFERDARTRIESRVKLSEGNYAAYAGT
                     ARAQARARSDLIVHSALAGVGIFLLLYIAFNNLHNLLLTFVNLPFALIGGVIAVFAGG
                     GWMSLGSLVGFVTLFGITLRNSIMLVSHYQHLVEVERLPWNLETAMRGAGERLPSILM
                     TALVTALALMPLALGSGEPGREIEGPMATIIVGGLATSTLLNLLILPTILLQFGRFAP
                     RDGSSER"
     gene            complement(3742..>4078)
                     /locus_tag="VE030_13435"
     CDS             complement(3742..>4078)
                     /locus_tag="VE030_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HZF24455.1"
                     /translation="GLPGETYFYVVDGGGFRAGMRVAARIGLGGVPVAGVIVPSQAVV
                     WHAGKAWVYLKKSEQTFARHEVSTVEELDGGWFNAGGLQPGDEVVVSGAQLLLSEELK
                     YQIRNENED"
BASE COUNT          803 a         1331 c         1277 g          667 t
ORIGIN      
        1 aagattgccg tggcggtccc gtgcagtccg aacatgtcga acagcccgcg gctgttgcga
       61 tagacctgcg cgaaagcaac ggcgggatgc ttcccgagga catcattcgc aagcgcggca
      121 aatgttgctg cgtcgctatc ctccccgaaa aagccgatca ccgccgtatc attgtcggtc
      181 acgaaacgat cgaaaccggt ttcgtcgagg acggtcggca ccatgaacgt catgagggcg
      241 tgccggcctc gaaggtcggg gtgaaggcgc ggcacgggcc gcaccacggc gcccagaaat
      301 ccacgatcac ggtgtagttg ccggtcacca cctgctcgaa gttgtctttg gtcaaagcca
      361 ctgtggccat caataggcgc tccttgcacg ccagtctatc tgcttggtta acctgccgaa
      421 agccggtcgc gccgtttagc gctcactcga accatcgcgc ggtgcgaacc tgccgaattg
      481 cagcaggatg gtgggcagga tcagcagatt caggagggtc gaagtggcga gcccgccgac
      541 aatgatggtc gccatcggac cctcgatttc ccgtcccggc tccccactgc ccagagccag
      601 cggcatcagc gccagtgcgg tgaccagcgc agtcatcagg atcgacggca ggcgttcgcc
      661 ggcgccgcgc atggcggttt ccaggttcca cggcagccgc tcgacctcga ccaggtgttg
      721 atagtgcgat accagcatga tcgagttgcg cagcgtgatg ccgaacagcg taacgaatcc
      781 caccagcgaa cccaacgaca tccagccgcc accggcaaac acggctatca cccctccgat
      841 cagcgcaaaa ggcagattca cgaaagtgag cagcaggttg tgtagattgt tgaaagcgat
      901 gtaaagcagc agaaaaatac ccacgccggc gagcgcagaa tgcacgatca ggtcggaccg
      961 ggcccgcgcc tgtgcccggg cggtgccggc atacgccgca taattgccct ccgacagttt
     1021 cactcgcgat tcgatgcggg tgcgtgcatc gcgctcgaac ttcgcaatgt cgcgggccac
     1081 cacattggcg gtcacggtct ggattcggcg ccccgcggag tgcagcacct tgtagcgccc
     1141 gccggtcatt tcgatggtgg ccacgtccgc aagcctgatc aggcgcccgt ccaggttgcg
     1201 caagggcagc gcaccgactt cgttgatgcg gttgcgatgc tgcggatcca gcagcacgac
     1261 caggtccacc acccggctgc cgcggtagat ctgcgcgact ctcgcgcctt cgtacgccgc
     1321 ctgcaccgct tccagcaccg tcagcggctg caaacccatt gcggcaacgc gctcgtgatg
     1381 caggcgcacc gacaactgcg gcgtgccggg cggcgcatgg atctgcacat cgtgcgcacc
     1441 cggcaccgac gccagcgtag tagcgatcgc cagcgcgtcc cggtcgaggg aatccagcga
     1501 ggaaccgtaa agattcacca cgaagggtgc ggcatagccg gtgatggtct cctcgatgcg
     1561 ctcggtgaga aaagtattta ccgcgaaggt cacacccggg aaggctccgc tgcgttcgcc
     1621 ggaaagcgtc gcgcggattt cgcgcaggat gcgcgcctgg tcctcgcccg ccagcgcgcc
     1681 gatttcgatt tcaaactcgc tgtagtgggt gccgaaggtg tcggcgccgt tcttcgagcg
     1741 gccgacccac tgcgcgaccg accgtacgcc ctcgattgag ccgatcgcct gcgacacctt
     1801 ctggccgata cgcagcgact ccgcttccga agtgcccggc accgcggaca tgtgcatgat
     1861 gtagtggcct tcgcgcagcg ccgggatgaa ttctcccgag aacagcggca gcatgccgat
     1921 gcctatcgcg atcaccacgc cggtaacgcc gatcacccgg ccgggatagc gctcgatacg
     1981 ccgcaagacg ccgagatagc gcggcttgag ccatttgacg aatggcgggt cgtcggattt
     2041 gagatccacg cgcgccagca gcagatagca cagcgcgggg gtgagcgtca ccgccaccac
     2101 cagcgaggcg aggatcgaga ggatataggc cagcccgagc ggggcgaaaa gctttccggc
     2161 gaccccgccc agcgtgagca ggggcacgaa taccagcgcg acgatgaacg tggcgtaaac
     2221 cacggaactg cgcacttcca cagaggcgcc cagcaccact tcccataccg gccgcggctt
     2281 cgaggccgtg cggttcaggc gcaggcggcg gaaaatgttc tcggtgtcga tgatggcgtc
     2341 atcgaccact tcgcccacgg caatggccag gccgcccatc accatgatgt tcaggccgat
     2401 gccgaaatag tcgagcacca gcaccgcggt gagcaacgat accgggatcg cagtggcgca
     2461 gatgaaagcg gtacggctgt tgaacaggaa caggaacaac accacgatca ccagagtcga
     2521 accgatcagg atgtcgcgct ggacgttgcg caccgcggtt tcgatgaagt tggcgggccg
     2581 gaacaggttc ggatgcaggc gcacccgctc ggcgtccagc aatggtttca gttccgccag
     2641 cgccgcttcc agatcctggg tgaccgcatg ggtgttggcg ccgagctggc cctggatcat
     2701 caggaatacg ccgggcgtgc cgtcgatcgc ggcggcgccg atcgagggcg ccgcaccctc
     2761 ggcaacctgc gccacgtcgc cgattcggat cgcgcgcccg tcgcgataag ccaggatcgt
     2821 gcgcgcgaca tccgacaacg catcgggctg cgcatcggtc tgcaacatga tgtgctggtt
     2881 cggcgtcttg atgaaacccg tgccggcaac gcccgcggcc gtgcgcgccg ccgcgaccgc
     2941 gtcttgcagc cccaggccat agcgctgcaa ccggcccgga tcgatccgga tctgccattg
     3001 cagcacgtcg ccgccgaaca cgttgacttc cgcgacaccg cgcaccgcca gcagatgcgg
     3061 ccggatgctc cagtccacca gtgtgcgcag gtccatcagg ctgcgccgct ccgaggtcac
     3121 gccgatgccg agcaccgtgc ttgcggaaga ggtgagcggc gtcatcgtcg gcaccacgcc
     3181 tttcggcagc tgggccgcga gcgaggccag gcgctcgccg accacctgcc ggttgaggta
     3241 gacatccgaa ccgtcccgga agaacacgtg caccacggac aatccgggga tcgactgcga
     3301 gcgcaggctt tccagccccg acgcgccgga caacgcgttc tcgatcgact gcgagacctg
     3361 ggtctcgacc agttcggccg acaggcccgg agcttcggtc tggatgacca cttgcgccgg
     3421 cgaaaactcg gggaacacgt cgaggtgcgc gcgcgacagc gtgaacaaac cgtaggcgac
     3481 gatggtcagc gccagcgcga cgatcacgcc tggataacgg atggactggc ggacgatgaa
     3541 ggagatcaat ttaagggtgt cctaaccaca gaggcacaga gaactacaac ccattcaaaa
     3601 cgaatttcaa gaaggagcaa atcaaggcac gtcgagggaa cgatgtccct gatgagctgc
     3661 gggtcctgac atatcgaatg ggcttgttgt cattccggtt tttctctgtg cctctgtgtc
     3721 tctgtggtga aggtcttagt cttagtcttc gttctcgttg cggatctggt acttgagttc
     3781 ctcggacagc agcaactggg cgccgctgac cacgacctcg tcgccgggct ggaggccgcc
     3841 ggcgttgaac cagccgccgt cgagttcttc cacagtggag acttcatggc gcgcgaaagt
     3901 ctgttcgctc ttcttcaggt aaacccaggc cttgccggcg tgccagacca ctgcctgtga
     3961 cggtacgatc accccggcga cgggcacccc gcccaagccg atccgcgccg cgacccgcat
     4021 gccggcgcgg aagccgccgc cgtccaccac ataaaaatag gtttccccag gcagcccc
//
LOCUS       DASQOP010000346         3336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1388746, whole genome shotgun sequence.
ACCESSION   DASQOP010000346 DASQOP010000000
VERSION     DASQOP010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1388746"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="VE030_13440"
     CDS             complement(<1..665)
                     /locus_tag="VE030_13440"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZF24456.1"
                     /translation="MLRAEHLSLGYPGRTLLEGLTLEFRAGQVWAVLGRNGSGKSTLL
                     HALAALRLPQAGVALLDGAPLDSMPRRRLARSVGVLLQEETHEFWGSVRDFVLLGRYP
                     HALSPFGWHMDDEAVARAEIESLHLAGLTDRAFATLSGGERQRARAAALFAQRPLVYL
                     LDEPLQHLDLPHQVAVLERLAREAREHGALVIMVVHDLLFASRYCDRFLLLYGDGRFA
                     HGGA"
     gene            complement(652..1629)
                     /locus_tag="VE030_13445"
     CDS             complement(652..1629)
                     /locus_tag="VE030_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HZF24457.1"
                     /translation="MPRALGLVFLLFIAGASVLVSLAAGSVPIPVEDTFAALSGSLSG
                     PTADIVWKLRLPRILAAFSCGGLLAMAGVLLQVLLRNPLADPYIFGVSGGAALGVLLA
                     MLLGTGTMIQTGSGLAGAIAASALVFGLSFRTGNWNPYRLLLTGVVVAAGLNAALSLI
                     LVLAPQTAIKGMLFWLMGDLGYAEHPLPALLVLMTLLIVATPLAQAMNVLALGRIKAR
                     SLGVAVTGTEILLYGLAALATVTAVLFGGTIGFVGLIVPHLMRMLGMADHRWLLPSAV
                     LAGGSFLMIADTIARSAWAPLQFPVGVLTALLGVPVLLLLLAGRGNAAR"
     gene            complement(1768..2424)
                     /locus_tag="VE030_13450"
     CDS             complement(1768..2424)
                     /locus_tag="VE030_13450"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZF24458.1"
                     /translation="MRLALFDLDNTLLAGDSDFEWARFLIEKGALDGEVHEARNRNFY
                     EAYKAGTLDIHEFLDFQLKPLSRHPRKQLDEWHVEFMQEKIVPIITQKARDLVESHSE
                     DARAIITATNSFVTWPIAREFGIENLIATEAEEVDGEFTGRVIGMPSFREGKVTRLMS
                     WMTEHGISWDALEETWFYSDSLNDLPLLSIVSHPVAVDPDETLKEHALKHDWRVISLR
                     "
     gene            complement(2639..>3336)
                     /gene="hda"
                     /locus_tag="VE030_13455"
     CDS             complement(2639..>3336)
                     /gene="hda"
                     /locus_tag="VE030_13455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HZF24459.1"
                     /translation="QLALGLAPSPVPAFDNFFPGRNVEAVSALQSLATGRSAEHFVYL
                     WGARGCGRSHLLQAAVAAASSRGIIARYVVAGKPLPVEDSGVRMLAVDDVEKLNAQSQ
                     LEFFHLYNALRDCQGAVLAAGNVAPARLQLRADLLTRLGWGLVFQVHALSDDDKRVAL
                     KRHAAARAFDLRDEIVEYLLRHLKRDLPSLMTVLEALDRYSLETKRPITLPLLRELLQ
                     SEGDGNENPPPGT"
BASE COUNT          665 a         1093 c         1049 g          529 t
ORIGIN      
        1 gcgccgccgt gcgcgaatcg cccgtcgccg tacagcagca ggaaccggtc gcaatagcgg
       61 cttgcaaaca gcaggtcgtg gaccaccatt atcaccagcg cgccgtgctc gcgggcctca
      121 cgcgccaggc gctcgagcac cgccacctga tgtggcaaat cgagatgttg aagcggttcg
      181 tccagcaggt atacgagcgg ccgctgggca aacaatgccg ccgctcgcgc gcgctggcgc
      241 tcgccgccgg aaagcgttgc gaatgcccgg tcggtcaggc cggccagatg cagggactcg
      301 atctcggcac gggccaccgc ctcgtcgtcc atgtgccagc cgaacgggct gagtgcatgc
      361 ggatagcggc ctagcagcac gaagtcgcgc acgctgcccc agaattcgtg cgtctcttcc
      421 tgcagcagca cgccgacgct gcgcgccagc cggcgacgcg gcatcgagtc gagcggcgcg
      481 ccgtcgagca gcgctacgcc ggcctgcggg agcctcaacg cagccagcgc gtgcaacagc
      541 gtggacttgc cgctgccatt gcggccgagc accgcccaca cctgtcccgc gcggaactcc
      601 agcgtcaggc cttcgagcag cgtgcgtccc gggtacccca gcgacaggtg ttcagcgcgc
      661 agcattgccc cggcccgcaa gaagcaacag caacaccgga acgccgagca gtgcggtcag
      721 aacaccgacc ggaaactgca aaggcgccca ggcgctgcgc gcgatcgtgt ccgcaatcat
      781 gagaaagctg ccgccggcca ggactgcaga cggcaacagc cagcggtggt cggccattcc
      841 cagcatgcgc atgagatgcg gcacgatcag tccgacgaac ccgatggtgc cgccgaacag
      901 caccgcggta accgtcgcca gcgcagcaag accatacaga agaatttcgg tcccggttac
      961 cgccacaccc agcgaccgcg ccttgatcct gccaagcgca agcacgttca tcgcctgcgc
     1021 cagcggcgtg gcgacaatga gcagtgtcat gagcaccaga agcgcaggca gcggatgctc
     1081 cgcatacccc agatcaccca tcagccagaa cagcattcct ttgatcgccg tttgcggcgc
     1141 cagcaccaga atcaggctga gggccgcgtt cagtcctgcc gcgacgacca ctccggtaag
     1201 tagcaggcga tagggattcc agtttccggt acggaaactc aaaccgaata ccagggccga
     1261 cgcggcgatc gcgccggcca gccccgatcc ggtctgaatc atcgttccgg tccccagcag
     1321 catcgccagc agcacgccga gcgcggcgcc cccggacacg ccgaagatat aaggatcggc
     1381 aagagggttg cgcagcaaca cctgcagcag cacccccgcc atcgccagca gaccaccgca
     1441 ggagaatgcc gccaggattc gtggcagccg cagcttccat acgatgtcgg ccgtgggtcc
     1501 ggagagcgaa ccggacagcg ccgcaaacgt atcctcgacg gggatgggga cgctgcccgc
     1561 cgccaggctt acgagcacgc tggcgccggc gataaacagc aggaagacga gaccgagtgc
     1621 gcgcggcatc ctagtgtcct gttccgctaa ttcgcggctg aaaagccgac gagaatctgc
     1681 gccatgctgt gttaagagtc ctggccgtgc gcgtttgagg gcggaacagg acaccagagc
     1741 atcaacggcg ccgcccccgc ccggcggtta acgcaggctg atgacgcgcc agtcgtgctt
     1801 gagcgcgtgt tccttgaggg tttcgtcggg atccaccgcg accgggtggc tgacgatcga
     1861 aagaagcggc agatcgttga gcgagtcgct gtaaaaccag gtctcctcca gcgcatccca
     1921 ggagatgccg tgctcggtca tccagctcat gagcctggtt accttgccct cgcggaagga
     1981 cggcattcct atcaccctgc cggtaaactc gccgtccact tcctccgctt cggtggcgat
     2041 caggttttcg ataccgaact cgcgcgcaat cggccaggta acgaaactgt tggtcgcggt
     2101 gatgatggcg cgcgcatcct cgctgtggct ttccaccaga tcgcgcgctt tctgagtgat
     2161 gatcgggacg atcttctcct gcatgaactc gacgtgccat tcgtcgagct gctttctcgg
     2221 gtggcgcgag agcggcttga gctggaaatc cagaaactcg tggatgtcga gcgtgccggc
     2281 tttgtaagcc tcgtagaagt tgcggttgcg cgcctcgtgc acctcgccgt cgagcgcgcc
     2341 tttctcgatg agaaaccgcg cccactcgaa gtcgctgtcg ccggcgagga gtgtgttgtc
     2401 gagatcaaac aatgcgagtc gcatggtctt cgcgcggggg cgtattgtgg ctccggcata
     2461 acgttagctt tgcgaacatt agcctccacc caaacaagct gctttgcagc gtgttttcaa
     2521 gatcaaacag tgcgagccgc attggggaat tgcggaagag gtcggagggg tcgtgaagaa
     2581 gcgcaggaag tatagctcaa aagcccgagc aggccgaagt tccggacccg ttcgccggtc
     2641 aggttcctgg cggcggattt tcatttccgt caccctccga ctgcagtagt tcgcgcagca
     2701 acggcaaggt gatcggtcgc ttggtttcca gcgagtagcg gtccagcgcc tccagcaccg
     2761 tcatcaggga gggcaaatcg cgcttgagat ggcgcagcag gtactcgacg atttcgtcgc
     2821 gaagatcgaa ggcgcgggcg gccgcgtggc gtttgagcgc gacgcgcttg tcgtcgtcgg
     2881 aaagcgcatg cacctggaac accagccccc atccgagccg ggtcagcagg tccgccctga
     2941 gttgcagccg tgccggcgcc acgttgccgg cagcgagcac ggcgccctgg caatcacgca
     3001 aggcattata cagatggaaa aactcgagct gggactgcgc attcagtttc tcgacgtcgt
     3061 ccactgccag catgcgcaca ccggagtcct ctaccggcaa cggcttgccc gccacgacgt
     3121 atcgggcaat gattccccgc gaggatgccg ccgccacggc agcctgcaat aaatgcgacc
     3181 ggccgcaacc gcgcgctccc cagaggtaaa cgaaatgctc ggcggagcgg cccgtggcaa
     3241 gcgactgcaa tgcggaaacg gcctcgacat tccgccccgg gaaaaaattg tcgaacgccg
     3301 gcaccgggga tggggccagt ccgagagcca gctgcc
//
LOCUS       DASQOP010000347        10590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2600134, whole genome shotgun sequence.
ACCESSION   DASQOP010000347 DASQOP010000000
VERSION     DASQOP010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10590
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2600134"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /gene="gidA"
                     /locus_tag="VE030_13460"
     CDS             <1..218
                     /gene="gidA"
                     /locus_tag="VE030_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452742.1"
                     /note="GidA; glucose-inhibited cell division protein A;
                     involved in the 5-carboxymethylaminomethyl modification
                     (mnm(5)s(2)U) of the wobble uridine base in some tRNAs;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HZF24460.1"
                     /translation="LDYRSVRGLSIEVQQKLNKHKPETLGQAARISGITPAAISLLLV
                     HLKRGLKTTAEPGAGHRSHVHAAGNRG"
     gene            215..859
                     /gene="rsmG"
                     /locus_tag="VE030_13465"
     CDS             215..859
                     /gene="rsmG"
                     /locus_tag="VE030_13465"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HZF24461.1"
                     /translation="MSQAAELLRQGIADLGLALPQLATERLLDYLVLLAKWNRVYNLT
                     AIREETKWVSHHLLDSLAVVPHLPAGRVVDVGSGAGLPGIPIAFACPDRQVTLLDSNQ
                     KKGAFLTQSSTELALTNVKVVIERAESYRPGTTFDVVISRAFSSIADFIRLAGHLRTP
                     GGVLLAMKGAHPEAEIAQLPDSWKAKAIIPLQVPRLNAKRHLVMLRPGAAADAS"
     gene            865..1629
                     /locus_tag="VE030_13470"
     CDS             865..1629
                     /locus_tag="VE030_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZF24462.1"
                     /translation="MLRILAISNQKGGVGKTTTSINLAASLVMAGRRVLLVDIDPQGN
                     ATMGSGIDKRSLRATVYQVLLGSAPMSSVRVRSQSGGYDLIPANRELAGAEVEMVELA
                     ERETRLKKALEEIKAQYDYVLIDCPPALNLLTVNGLCAANAVMIPMQCEYYALEGLSD
                     LVQTIKKVRMNLNPTLEIEGLLRTMYDPRNMLSQQVSDQLQQHFGDKVYRTVIPRNVR
                     LAEAPSHGIPALSFDRQSKGAMAYLALAGEMLGKNT"
     gene            1641..2486
                     /locus_tag="VE030_13475"
     CDS             1641..2486
                     /locus_tag="VE030_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014895494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HZF24463.1"
                     /translation="MAKLKGLGRGLDALLAGNDGRVEDAQAELAVGMLKPGRFQPRTR
                     MDPQSIAELADSIRAQGVIQPILVRPVDGGKYEIIAGERRWRAAQLAGLAQVPVVVRS
                     VPDQAALALALIENIQREDLNPLEEAGGIHRLIDEFRMTHDTAAQAVGRSRSAVTNLL
                     RLLNLAKPVQELLMQGKLDMGHARALLALSGPRQVELAHQVVSRALSVRETEQLAGRA
                     EATVRKTPRKHRKDRDLQALEENLAETLGAPVTITPGKHGRGKLVIGYSSLDQLDEFL
                     RKLRR"
     gene            2626..2985
                     /locus_tag="VE030_13480"
     CDS             2626..2985
                     /locus_tag="VE030_13480"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HZF24464.1"
                     /translation="MIRAMVSIIAMQVAVTLGVAALFLLFGNVVSAKSALIGGAIAFI
                     PGAFYALPVIRSRHSAADRLLRAHYVGEFGKLMLTVLLFGATFAWVKEISVLPLFADY
                     IATLLVYWAALVMFSRI"
     gene            2992..3783
                     /gene="atpB"
                     /locus_tag="VE030_13485"
     CDS             2992..3783
                     /gene="atpB"
                     /locus_tag="VE030_13485"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004773235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HZF24465.1"
                     /translation="MSTEQATPTSTEYIVHHLTNLHVGHGFWTLHLDTLIVSGVLGLV
                     IFGMMALAARRATSGVPPGMQNFFEMVVSLVDSQVRDTFHAKSELVTPLAITIFVWVW
                     VMNAVDLIPVDFLPKVVHATGVEHFKFVPTTDPNLTFAMSITVFLIMLGFNVRFKGIG
                     GFLHEALTVPFGKYLFPVNLLFRIIEDIAKPISLALRLFGNMYAGEMVFILLAVWAGA
                     GFLWAPFSAVLSLGWALFHILIITLQAFVFMMLTIVYMSMAAESH"
     gene            3832..4107
                     /gene="atpE"
                     /locus_tag="VE030_13490"
     CDS             3832..4107
                     /gene="atpE"
                     /locus_tag="VE030_13490"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010393790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HZF24466.1"
                     /translation="MEIASVLGMTAIAVALLIGMGALGTAIGFGILGGRFLEGAARQP
                     EMIPTLQVKMFIVAGLLDAVTMIGVGIALYLLFANPFIAAVQQAVGG"
     gene            4137..4610
                     /locus_tag="VE030_13495"
     CDS             4137..4610
                     /locus_tag="VE030_13495"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HZF24467.1"
                     /translation="MNINATLLGQAIWFALFIWITMKFVWPPLRKAMHDRQKQIADGL
                     AAGERGKQDLELAAKRAAEELQRARAQASEIVSQAEKRGAGVVEEAKSAAKAEADRIV
                     AAAKAEIEQEVFRAKESLRAQISLLAVTGAEKILKREVDAKAHAQLLSGLESELR"
     gene            4618..5154
                     /locus_tag="VE030_13500"
     CDS             4618..5154
                     /locus_tag="VE030_13500"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HZF24468.1"
                     /translation="MAELATIARPYAEAVFRLAKEGNALGTWSEKLAFIAAVYHDPQM
                     QAAIGNPRVTANDVERLLLAISGDRIDDAARNFIQLLVRNRRLAMLPQIREQFEKLKL
                     EDEGKLDARISSAYPLDDAQRNQVVNLLSSRFKRKINATVTVDPELIGGIKVEVGDKV
                     WDASVRGRLQTMAVTLSR"
     gene            5167..6708
                     /gene="atpA"
                     /locus_tag="VE030_13505"
     CDS             5167..6708
                     /gene="atpA"
                     /locus_tag="VE030_13505"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004524507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HZF24469.1"
                     /translation="MQLNPSEISELIKNKIQNLATASEVRTQGTVVSVTDGICRIHGL
                     ADVMQGEMLEFPGNTFGLALNLERDSVGSVVLGEYEHISEGDPVKSTGRILEVPVGPE
                     LIGRVVNSLGQPIDGKGPIAAKETDVIEKVAPGVIWRKSVSQPVQTGLKAIDAMVPIG
                     RGQRELIIGDRQTGKTAVAVDTIINQKGKDLFCIYVAIGQKASTVANVVRKLEEHGAM
                     AYTIVIAATASESAAMQYIAPYSGCTMGEYFRDRGQDALIIYDDLTKQAWAYRQVSLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVSEAWVERFTKGKVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGVSVSRVGGAAQTKIMKRKDTG
                     GGVRLALAQYRELAAFAQFASDLDEATRKQLERGRMVTELMKQLQYQPLQVWEMALTL
                     FAVNNSYYDDIDVKKALAAEKSMRDYVKTKYAVLAGRMDKELSADDEKALHEAIKDWK
                     KNGSY"
     gene            6803..7666
                     /gene="atpG"
                     /locus_tag="VE030_13510"
     CDS             6803..7666
                     /gene="atpG"
                     /locus_tag="VE030_13510"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HZF24470.1"
                     /translation="MPGTKEIRTKIKSVQSTRKITKAMEMVAASKMRKVQERMRAARP
                     YAEKIRNVAAHISHANPEYRHPFLVERDTVKRVGIIIVTTDKGLCGALNTNVLRMMLN
                     HYKQWQSEGEEIDVCCIGGKGLGFMQRLGANIVSKSAGLGDRPKLENLIGAVKVMLDS
                     YTEDRFDRLLIFYTRFINTMKQEPVMEQLLPLSGERLGAPEGTWDYIYEPEAKTVLEQ
                     VLTRYIEAVVYQGVSENMASEQSARMVAMKSASDNAATLIDELTLIYNKSRQAAITKE
                     LSEIVGGAAAV"
     gene            7789..9213
                     /gene="atpD"
                     /locus_tag="VE030_13515"
     CDS             7789..9213
                     /gene="atpD"
                     /locus_tag="VE030_13515"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HZF24471.1"
                     /translation="MSQGTIVQCIGAVTDIQFPREAMPKIYDALALEESTDASFAEKG
                     LTFEVEQQLGDGIVRCIAMGSADGLRRGMKVKNTGAPISVPVGKGTLGRIMDVLGRPI
                     DEGGPIKTEERRSIHQTAPKFDELSPSVELLETGIKVIDLICPFAKGGKIGLFGGAGV
                     GKTVTMLELINNIATKHGGQSVFAGVGERTREGNDFYHEMIEAEVIKLDNLEESKVAM
                     VFGQMNEPPGNRLRVGLTGLTMAESFRDEGRDILLFIDNIYRFTLAGVEVSALLGRMP
                     SAVGYQPTLAEEMGRLQERITSTKTGSITSVQAIYVPADDLTDPSPATTFGHLDATVV
                     LSRDIASLGIYPAVDPLDSTSRQVDPNTIGEEHYNTTRAVQATLQRYKELRDIIAILG
                     MDELAPEDKLAVSRARKIQRFLSQPFSVAKNFTGQEGKYVQLKDTIKAFKMIVNGECD
                     SLPEQAFYMVGGIEEAFEKAKTLQ"
     gene            9228..9647
                     /locus_tag="VE030_13520"
     CDS             9228..9647
                     /locus_tag="VE030_13520"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HZF24472.1"
                     /translation="MANTIHVDVVSAEQQIFSGEALFVILPGEAGELGIYPRHTPLIT
                     RIKPGAVRIKPADGGEENLIFVDGGILEVQPSIVTVLADTAIRGHDLDEAKALEAQKR
                     AEEALRNAKDKQEIATVEAELSMLAAQLAAIRKLRRK"
     gene            complement(9782..>10590)
                     /locus_tag="VE030_13525"
     CDS             complement(9782..>10590)
                     /locus_tag="VE030_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HZF24473.1"
                     /translation="TGKLILRLQDPTEGQVLWRGRRIDQLDRAGMRPVRRELQAVFQD
                     PYSSLNPRMRAGDIVAEPIRNYESLPAAEVRLRTAHLFERVGLRADQLVKYPYEFSGG
                     QRQRLGIARALSVRPRLIVCDEPVSALDVSVQAQVINLLMDLQAEFGLSYLFVAHDLA
                     VVEHISHRVAVMYLGKIVEIAPKRAIFTSPQHPYTEALLEAVPIPNPTIRKQRRLLAG
                     DVPSAVHPPSGCRFHTRCPYVEERCKREEPPLQEISPQQWVACHLRQPIA"
BASE COUNT         2187 a         3207 c         3320 g         1876 t
ORIGIN      
        1 atctcgatta tcgcagcgtg cggggactgt cgatcgaggt gcaacagaaa ctcaacaagc
       61 acaagcccga gaccctgggc caggccgcgc gcatatccgg catcactccg gcggcgatct
      121 cgctgctgct ggtgcacttg aagcgcggcc tcaagacgac agccgagcct ggcgctggcc
      181 accgcagcca cgtacacgct gcgggaaacc gcggttgagc caggcggcag agctattgcg
      241 ccagggtatt gcagacctgg gattggcgtt gccacagttg gcgaccgagc ggctgctcga
      301 ctacctggta ctgctggcga agtggaatcg cgtttacaac ctcacggcaa tccgtgagga
      361 aacgaaatgg gtcagccatc acctgctcga tagcctcgca gttgtgccgc acttaccggc
      421 agggcgagtt gtggatgtgg gcagcggcgc agggttaccg gggatcccga ttgcgttcgc
      481 atgtccggac agacaggtga cgttgctcga ttcgaaccag aagaaaggcg cgtttttgac
      541 tcagtcatcc accgagctgg cgctcaccaa cgtcaaggtc gtaatcgaaa gagccgaatc
      601 gtaccgaccc ggcacgacat ttgacgtcgt gatctcgcgc gccttctcca gcatcgccga
      661 cttcatcagg cttgcaggcc acctgcgcac gcccggcggc gtgctgcttg cgatgaaagg
      721 cgcgcacccg gaagcagaga tcgcgcagct tccggactcc tggaaggcga aagcaatcat
      781 cccgttgcaa gtgccccggc tcaacgcgaa gcgccatctg gtcatgctgc gcccgggcgc
      841 agcagcggac gccagctgaa cagcatgctg cgcatacttg cgatcagcaa ccagaaaggc
      901 ggggtcggca agaccaccac cagcataaat ctcgccgcga gcctggtgat ggccgggcgg
      961 cgcgtgctgc tggtggacat cgatccgcag ggcaacgcca ccatgggaag cggcatcgac
     1021 aagcgcagcc tcagggcgac ggtctaccaa gtgttgctgg gtagcgcgcc gatgtcgtcg
     1081 gtccgggtgc gcagccagag cggcggctac gacctgatcc ctgccaaccg cgaactcgct
     1141 ggtgcggaag tggaaatggt ggaactggcc gaacgtgaaa cgcggctgaa gaaggcactc
     1201 gaagaaatca aggcgcagta cgactacgta ctgatcgact gcccgccggc gctcaatctt
     1261 ctgacggtga acgggctgtg cgcggccaac gcggtgatga ttccgatgca gtgcgagtac
     1321 tacgcgctcg aaggcctgtc tgacctggta cagaccatca agaaagtgcg catgaatctg
     1381 aatcccacgc tcgaaatcga gggtttgctg cgcaccatgt acgacccgcg caacatgctt
     1441 tcgcaacagg tttcggatca actccagcaa cacttcggcg acaaggtcta ccgtaccgtg
     1501 atcccgcgca atgtacggct tgcggaggcg cccagccacg gcatcccggc gctcagcttc
     1561 gaccggcaat cgaaaggcgc gatggcctac ctcgcactgg caggcgagat gctcggcaag
     1621 aacacttagg aaaaccgaag atggcgaaac tcaaaggact tggccgggga ctcgacgcac
     1681 tgctcgccgg caacgatggt cgggtcgagg atgcgcaggc cgaactcgcg gtgggcatgc
     1741 tcaagcccgg ccgcttccag ccgcgcacgc gcatggaccc gcagtcgatt gcggaacttg
     1801 ccgactcgat cagggcccag ggcgtgatcc agccgattct ggtgcgcccg gtggacggcg
     1861 gcaagtacga gatcatcgcc ggtgagcgcc gctggcgtgc ggcccagctt gccggactgg
     1921 cgcaagtgcc ggttgtagtg cgttcggtac ctgaccaggc cgcgctggcg ctggcgttga
     1981 tcgagaacat ccagcgcgag gacctcaatc cgctggaaga agcgggcggc attcacaggt
     2041 tgatagacga attccgcatg acccacgaca ccgcggcgca ggcagtcggg cgctcgcgca
     2101 gcgcggttac caatttattg cggctgctga atcttgccaa gcccgtgcag gaactgctga
     2161 tgcagggaaa gctcgacatg ggccatgccc gggctctgct ggcgcttagt gggccgcgcc
     2221 aggttgagtt ggcacaccaa gttgtcagcc gcgcgctgtc ggtgcgtgaa accgaacaat
     2281 tggctggccg tgccgaggcg accgtccgca agacgccgcg caaacacagg aaggatcgcg
     2341 accttcaggc cctggaggaa aaccttgcgg agacgctcgg cgcgccggtg accatcaccc
     2401 cgggcaagca cgggcgcggc aaactggtca tcggctactc cagtctcgat caactcgatg
     2461 aattcctccg caaactgcgc cgctgaacgg ccagcgcacc gcagcaaagc cagatcgtcg
     2521 ttgacagtgc ttcctcaacc ccactaaaat tttggccttt ttgcggattg ttgcggccgc
     2581 atcggtttca aaaatcccag cctcaggccg gtggcaggag ggacgatgat ccgggcgatg
     2641 gtcagcatca tcgccatgca ggtcgcagtg actttgggcg tcgccgcgct gttcctgttg
     2701 ttcgggaacg tcgtatccgc aaaatccgct ttgatcggcg gcgcgatcgc tttcatcccg
     2761 ggtgcgtttt acgcattgcc agtcatccgg tcccggcata gtgcagcgga tcgtttgctg
     2821 cgagcgcact atgtcggcga attcggcaag ctgatgttga ccgtgctcct gttcggcgcg
     2881 acgttcgcgt gggtgaagga aatttccgtt ttgccgttgt ttgccgacta catcgcgaca
     2941 ctattggtgt actgggcggc gctggtgatg ttttcgagaa tctgaaccga catgagcacc
     3001 gagcaagcaa cgccgacctc cacagagtac attgtccatc acctgaccaa cctgcacgtc
     3061 ggtcacggtt tctggacgct gcatctcgat accctgattg tctccggtgt cctcggtctg
     3121 gtgatcttcg ggatgatggc gctggccgcg cggcgcgcca ccagcggcgt gccaccggga
     3181 atgcagaact tcttcgagat ggtggtctcg ctggtcgatt cgcaggtgcg ggatactttc
     3241 cacgccaaga gcgagctggt cacgccgctg gcgatcacga tattcgtctg ggtgtgggtg
     3301 atgaacgctg tggacctgat cccggtcgat ttcctgccga aggtcgtgca cgcaaccggc
     3361 gtcgagcatt ttaaattcgt gcccaccacc gaccccaacc tgaccttcgc gatgtcgatt
     3421 acggtgttcc tgataatgct gggattcaac gtcaggttca agggcatcgg cggattcctt
     3481 cacgaagcgc tgaccgtgcc gttcggcaag tacctgtttc ccgtcaacct gctgttccgc
     3541 attatcgaag acatcgccaa gccgatctcg ctggcgctgc ggttgtttgg caacatgtac
     3601 gcgggcgaga tggttttcat cctgctcgcg gtgtgggcgg gcgcgggttt cctgtgggcg
     3661 cccttcagcg cggtgctgag cctcggatgg gcgctgttcc acatcctgat catcacgctg
     3721 caggcctttg tgttcatgat gctgaccatc gtgtacatga gcatggcggc ggaaagccac
     3781 taaaacgcaa cgagcaattg tctttaatcg tctagcactg ggagaacaaa aatggaaatc
     3841 gcatcagtat tgggtatgac ggccatcgcg gtggcgctgc tgatcggcat gggcgcgttg
     3901 ggtaccgcga tcggcttcgg cattctcggg ggccgcttcc tcgagggcgc cgcgcgtcaa
     3961 cccgagatga tcccgacgct gcaagtgaag atgttcatcg tcgccggcct gctcgatgcg
     4021 gtgaccatga tcggcgtggg tatcgcgctc tatctgctgt tcgccaatcc attcatcgcg
     4081 gcagtccagc aggcagtcgg cggctaaatc tggagcattg agcccgggag gcgagcgtga
     4141 atatcaacgc tacattgttg ggccaggcaa tctggttcgc ccttttcatc tggatcacga
     4201 tgaagtttgt ctggccgccg ctgcgcaagg cgatgcacga tcgccagaag cagatcgccg
     4261 acggccttgc cgcgggggaa cgcggcaagc aggatcttga gcttgcggcg aagcgcgcgg
     4321 ccgaagagct gcagcgggcg cgtgcgcagg cgagcgagat cgtttctcag gccgagaagc
     4381 gcggagccgg cgtggtcgaa gaggccaagt cggccgccaa ggccgaggcc gaccgcatcg
     4441 tcgccgccgc caaggcagag atcgagcagg aggtgttccg cgccaaggag tcgctgcgag
     4501 cacaaatatc gctgctggcg gtgaccggcg ccgaaaagat cctcaagcgc gaggtggacg
     4561 ccaaggcgca cgcgcagttg ctgtccggcc tcgagtccga actcaggtaa cgcccgcatg
     4621 gccgaactcg caaccattgc caggccttac gcggaggcag tgttccgcct tgccaaggaa
     4681 ggcaacgcac ttggcacctg gtcggaaaag ctggcattca tcgccgcggt ctaccatgat
     4741 ccgcagatgc aggcggcgat cggcaatccc agggtcactg ccaacgacgt cgaacgcctg
     4801 ctacttgcca tcagcggcga ccgcatcgat gatgcggccc gcaacttcat tcagttactg
     4861 gtgcgcaacc gccggctagc gatgctgccg cagatacgcg agcagtttga gaaactgaag
     4921 ctggaagacg agggcaagct ggacgcaaga atcagttccg catacccgct cgacgacgcc
     4981 caacgcaatc aagtggtgaa cctgctgtcc tcccgcttca agcgcaagat taacgccacc
     5041 gtgaccgttg accccgagtt gatcggcggc atcaaggtcg aagtgggcga caaggtttgg
     5101 gatgcctcgg tacgcggcag gctgcagacc atggccgtga ccctttcgag ataggagttt
     5161 tgaaccatgc agttgaaccc gtccgagatc agcgaactga tcaagaacaa gatccagaat
     5221 ctggccaccg cttccgaggt tcgcacgcag ggtacggtgg tgtccgtaac cgatggcatc
     5281 tgtcgcattc atggccttgc cgacgttatg cagggtgaaa tgctggagtt tcccggcaac
     5341 accttcggcc tcgcgctcaa cctcgagcgc gactcggtcg ggtcggtggt gctgggcgaa
     5401 tacgagcaca tttccgaagg cgatccggtg aagagcaccg gccgcattct ggaggtgccg
     5461 gtcggtcccg aactgatcgg gcgcgtggtc aactcgttgg gccagccgat cgacggcaag
     5521 ggcccgatcg ccgccaagga aaccgatgtg atcgagaagg tcgctccggg cgtgatctgg
     5581 cgcaagtcgg tatcccagcc ggtgcagacg gggctcaagg cgatcgatgc gatggtaccg
     5641 atcggccgtg gccagcgcga gctgatcatc ggcgaccgcc agaccggcaa gaccgcggtc
     5701 gcggtggaca cgataatcaa ccagaagggc aaggacctgt tctgcattta tgtcgcgatc
     5761 ggtcagaagg cttccacggt cgccaacgtc gtgcgcaagc tcgaagagca cggcgcaatg
     5821 gcttacacca tcgtcatcgc ggccacggca tccgagtctg cggcgatgca gtacatcgcg
     5881 ccctattccg gctgcaccat gggcgagtac ttccgcgatc gcggccagga cgcgctgatc
     5941 atttacgatg atttgaccaa gcaggcctgg gcctatcgcc aggtatcgct gttgctgcgg
     6001 cgcccgcccg gacgcgaagc ctaccccggc gacgtgttct acctgcactc ccggctgctg
     6061 gaacgcgccg cgcgcgtaag cgaggcctgg gtcgagcgct tcaccaaagg aaaggtgaag
     6121 ggaaaaaccg gttcgctcac cgcgctgccg atcatcgaaa cccaggccgg cgacgtatct
     6181 gcattcgtgc cgaccaatgt gatttccatc accgacggtc agattttcct ggaaactgac
     6241 ctgttcaacg ccggcatccg tcccgcgatc aacgccggcg tatcggtgtc gcgggtcggt
     6301 ggcgcggcgc agaccaaaat catgaagcgc aaggacaccg gcggcggcgt gcgcctggcc
     6361 ctggcgcagt accgcgagtt ggcggcgttt gcccagttcg cttccgatct cgacgaagct
     6421 acccgcaagc agctcgagcg cggccgcatg gtcacggagt tgatgaagca actgcagtac
     6481 cagccactgc aggtttggga aatggcgctg acgctgttcg cggtcaacaa cagctattac
     6541 gacgacatcg acgtcaagaa ggcgttggcc gcggagaagt ccatgcgcga ctacgtcaag
     6601 accaagtatg cagtgcttgc cggtcgcatg gacaaggaac tgtccgccga cgatgaaaaa
     6661 gcgctgcacg aggcgatcaa ggactggaag aagaacggca gttattgacg gacatcctcg
     6721 ggatgccgca ataactgcgc gcgtttgaac gaacgacaag ttgtcgctca acgcgcgcgg
     6781 cacggcaatc ggaaactaga ctatgcccgg cacaaaagag atacgtacca agatcaagag
     6841 tgttcagagc actcgcaaga tcaccaaggc gatggagatg gtcgcggcat ccaagatgcg
     6901 caaggtgcag gagcgcatgc gcgcggcccg tccatacgcc gagaagatcc gtaacgtcgc
     6961 ggcacacatt tcgcacgcca atcccgagta tcgccatcct tttctggtgg agcgcgatac
     7021 ggtcaagcgc gtcggcatca tcatcgtcac caccgacaag ggattgtgcg gcgcgctcaa
     7081 taccaatgta ctgcgcatga tgctcaacca ttacaagcag tggcagtccg agggagagga
     7141 gatcgacgtc tgctgcatcg gcggcaaggg tctcggcttc atgcagcgct tgggcgcgaa
     7201 catcgtttcc aagtccgctg gcctgggcga tcgtccgaaa ctcgagaatc tgatcggcgc
     7261 ggtcaaggtc atgctcgaca gctacaccga agaccgtttc gaccgtttgc tgattttcta
     7321 cacccgtttc atcaacacca tgaagcagga accggtgatg gagcagttgc tgccgctgtc
     7381 cggcgaacgt ctcggcgcgc ccgagggaac ctgggactac atctacgaac cggaggcgaa
     7441 gaccgtgctc gaacaggtac tgacccgtta catcgaggcg gtagtttatc agggcgtttc
     7501 ggagaacatg gcatccgagc aatcggcgcg catggtggcg atgaagtccg cttccgacaa
     7561 cgcggcgacg ctgatcgacg aactgaccct gatctacaac aagagccgcc aggcggcgat
     7621 caccaaggag ctgtccgaga tcgtcggcgg cgccgcggcc gtctgaattt cattgcacca
     7681 cagagacaca gagtgcacag aggcgaggca agaacgagac aaggattttg ccgtgcgctc
     7741 ggggcctctg tggtaaaccg aatttgatct agactttagg aaacgataat gagccaagga
     7801 accattgttc agtgcatcgg cgcggtgacc gacatccagt ttccacgcga ggcgatgccc
     7861 aaaatctacg acgcgctggc gctagaagaa tcgaccgacg cttcgtttgc cgaaaaggga
     7921 cttaccttcg aagtcgagca gcagctcggc gacgggatcg ttcgctgcat cgcaatgggc
     7981 tcggccgacg gcctgcgccg cggcatgaag gtaaagaaca ccggcgcgcc catctcggtg
     8041 ccggtcggca aggggacgct cgggcgcatc atggacgtgc tcggccgccc gatcgacgag
     8101 ggcggcccga tcaagaccga ggagcgccgt tcgatccacc agaccgcgcc aaagttcgac
     8161 gagctttccc cgtcggtcga gctgctcgag accggcatca aggtgatcga cctcatctgc
     8221 ccgttcgcga agggcggcaa gatcggcctt ttcggtggcg ccggcgtcgg caagaccgtg
     8281 accatgctcg agctcatcaa caacatcgcc accaagcatg gcggccaatc ggtgttcgcc
     8341 ggcgtgggcg agaggacccg cgagggcaac gatttctacc acgagatgat cgaggcggaa
     8401 gtcatcaagc tcgacaacct cgaggagtcc aaggtcgcga tggtgttcgg ccagatgaac
     8461 gagccgccag gcaaccgcct gcgcgtgggc ctcaccggcc tcacgatggc ggaatcgttc
     8521 cgcgacgaag ggcgcgacat cctgctcttc atcgacaaca tctaccgctt cacactcgcc
     8581 ggtgtggaag tgtcggcgct gcttggccgg atgccttccg cggtgggtta ccagccgacg
     8641 ctcgcggagg agatgggccg gctgcaggag cgcatcacct cgaccaagac cgggtcgatc
     8701 acctcggtgc aggcgattta cgttccggcc gacgacctga ccgatccgag ccccgcgacc
     8761 accttcggcc atctggatgc cacggtggtg ctgtctcggg acatcgcgtc gctcggaatc
     8821 tatcctgccg tggacccgct cgattcgact tcgcgccagg tcgatccgaa caccatcggc
     8881 gaagagcact acaacaccac gcgcgccgtg caggcgacgc tgcagcgcta caaagagctg
     8941 cgcgacatca tcgcgatcct cggcatggac gagctcgcgc ccgaggacaa gctcgccgtc
     9001 tcgcgcgcgc gcaagatcca aagattcctg tcgcagccgt tcagcgtggc gaagaacttt
     9061 actggccagg aaggcaagta cgtgcaactc aaggacacga tcaaggcctt caagatgatc
     9121 gtcaatggcg aatgtgacag cctgcccgag caggccttct acatggtcgg cggcatcgag
     9181 gaagccttcg agaaagccaa aacgctgcag taaggcgaaa gtcgacaatg gccaatacca
     9241 tccatgtgga cgtcgtcagc gccgaacagc agatcttctc cggcgaggcg ctattcgtga
     9301 ttctgcctgg cgaggccggg gaactaggca tctatccccg gcacacacca ttgatcacgc
     9361 gcatcaagcc gggcgcggta cgtatcaagc ccgcggacgg cggcgaagag aacctgatat
     9421 tcgtggacgg cggcattctc gaagtgcagc cgtcaattgt gaccgtgctg gccgataccg
     9481 cgattcgcgg ccacgatctt gatgaggcca aggcgctgga agcgcagaag cgcgccgagg
     9541 aagcactgcg caatgccaag gacaagcagg aaatcgccac tgtcgaagct gagctctcaa
     9601 tgctcgcagc gcaattggcg gcgattcgca agctgcgcag gaagtagacc ggatcgtgtg
     9661 tgacgaagga gcgggccgcg gcccgctttt tttttgtcag ttgacgcgcg ttgccaatat
     9721 cggcgtcagc caatgacctc gcgggtgacc tgcggcaaac gagagcttca gtcttgtgct
     9781 ttcaggcgat cggttgccgc aaatggcacg ccacccactg ctgcggagaa atctcctgta
     9841 acggcggctc ctcgcgcttg caacgctcct cgacataagg gcagcgcgtg tgaaagcggc
     9901 aaccggaggg cggatggacc gcgctcggca catcgcccgc gagcaagcgc cgctgcttgc
     9961 gtatcgtcgg gttcggtata ggcacggctt ccagcagcgc ttcggtgtat ggatgctgcg
    10021 gactcgtaaa aatcgcccgc ttcggcgcga tctcgacgat cttgcccaga tacatcaccg
    10081 caacacgatg gctgatgtgc tcgaccacgg cgagatcgtg cgcgacgaaa agatacgaga
    10141 ggccgaactc cgcttgcagg tccatgagca gattgatcac ctgcgcctgc acggacacgt
    10201 cgagcgccga cacaggttcg tcacacacga tcaagcgcgg ccgcaccgac agcgcccgcg
    10261 caatgccgag ccgctggcgc tggccacccg agaattcata tgggtacttc accaactgat
    10321 cggcacgcag gccgacgcgc tcgaacaggt gcgccgtgcg tagccgcacc tcggccgcag
    10381 gcaacgattc gtaattccgg atcggttcgg ccacgatgtc gcccgcgcgc atgcgcgggt
    10441 tgagcgagga gtagggatcc tgaaacacag cctggagctc gcgcctcacc ggccgcatac
    10501 cggcgcggtc gagttgatcg atacgccggc cacgccagag cacctgaccc tcggtcgggt
    10561 cctgcaaccg caggattagc ttgccggtgg
//
LOCUS       DASQOP010000348         2151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2600195, whole genome shotgun sequence.
ACCESSION   DASQOP010000348 DASQOP010000000
VERSION     DASQOP010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2600195"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..1799
                     /gene="aspS"
                     /locus_tag="VE030_13530"
     CDS             15..1799
                     /gene="aspS"
                     /locus_tag="VE030_13530"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HZF24474.1"
                     /translation="MRTEYCGLVSTRHLDHTVTLYGWVHRRRDHGGVIFIDLRDREGL
                     LQVVCDPDNARTFATAEKIRNEYVVKVTGLVRRRPAGTVNPNLVSGEVEVLARSLEIL
                     NPSVTPPFQMDDDNLSETVRLEYRYLDLRRPQMQENLRLRYRVAMAVRHFLDTQGFID
                     VETPMLTKSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDIETSFLGVAEITLLMEEMIRSLFKEVLDATLPNPFPRMSFAE
                     ATSKYGSDKPDLRVPLTLTELTDAMREVEFKVFRDAAVAPGGRVAALRVPGGGTLSRK
                     EIDDYTQFVAIYGAKGLAYIKVNDVAKGVQGLQSPILKFLPESVVKIVLERSGAKDGD
                     LIFFGADNAKVVNEALGALRTKVGHERGLAEKGWRPLWVVDFPMFEWSAEDKRWDALH
                     HPFTSPMDGHEDYLDRDPGKALSKAYDMVLNGWEIGGGSVRIHRQDVQSKVFRALGIG
                     AEEAQKKFGFLLEALQFGAPPHGGLAFGLDRIVTMMAGVESIRDVIAFPKTQRAQDLM
                     CKAPGPVDEKQLRELHIRLRNVEPVKAN"
     gene            1966..>2151
                     /gene="nudB"
                     /locus_tag="VE030_13535"
     CDS             1966..>2151
                     /gene="nudB"
                     /locus_tag="VE030_13535"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HZF24475.1"
                     /translation="MKFKIPISVLVVIHTPDLQVLLLERADHPGFWQSVTGSQDEGET
                     LRQTAAREVAEETGLDAE"
BASE COUNT          416 a          640 c          707 g          388 t
ORIGIN      
        1 ccgatcctga acttatgcgc accgaatact gcggactcgt ttccacccgg catctcgacc
       61 acaccgtcac gctctacggc tgggtgcacc ggcgccgcga ccacggcggc gtgatcttca
      121 tcgacctcag ggaccgggaa ggcctgctgc aggtggtgtg cgatccggac aatgcccgga
      181 cattcgccac cgcggaaaag atccgcaacg agtacgtggt gaaagtcaca gggctggtgc
      241 gccgccgccc cgcagggacc gtcaatccga acctggtttc cggtgaagtg gaagtgctgg
      301 cgcgctcgct ggaaatcctc aatccgtcag tcacgccgcc gtttcagatg gacgatgaca
      361 acctgtcgga aacagtgcgg ctcgagtacc gctacctcga cctgcgccgg ccgcagatgc
      421 aggagaacct gcggttgcgc taccgggtgg cgatggcggt gcggcatttc ctcgacaccc
      481 agggcttcat cgacgtggag acgccgatgc tcaccaagtc cacgccggaa ggcgcgcgcg
      541 actacctggt gccgagccgt gtgcacccgg gagagttttt cgcgctgccg cagtcgccgc
      601 agttgttcaa gcaattgttg atggtggcgg gcttcgaccg ttactaccag attaccaaat
      661 gcttccgcga cgaagacctc agggccgacc gccagccgga attcacccag atcgatatcg
      721 agacttcctt cctcggcgtg gccgagatca cgctgttgat ggaagaaatg atccgcagcc
      781 ttttcaagga agtgctggat gcgacgttgc cgaacccctt tccgcgcatg agcttcgcgg
      841 aggcgacgtc gaagtacggc tccgacaaac cggatctgcg cgtgccgctc acgctcacgg
      901 aactcaccga cgcgatgcgc gaagtcgaat tcaaggtttt ccgcgacgcg gctgttgcgc
      961 cgggcggccg tgtggcggcg ctgcgggtgc cgggcggcgg cacgctgtcg aggaaggaaa
     1021 tcgacgacta cacgcagttc gtcgccatct acggcgccaa gggtctggcg tacatcaagg
     1081 tcaacgatgt agcgaaaggc gtgcagggcc tccagtcacc gatcctgaaa ttcctgcccg
     1141 agtccgtggt gaagatcgtc ctggagcgca gcggcgcgaa ggacggcgac ctgattttct
     1201 tcggcgccga caatgccaag gtagtgaacg aagcgcttgg tgcgctgcgc accaaggtcg
     1261 gacacgagcg cgggctggcc gagaagggtt ggcggccgct gtgggtggtg gatttcccga
     1321 tgttcgaatg gagcgcggag gacaagcgct gggacgcgct gcatcaccct tttaccagcc
     1381 cgatggacgg ccacgaggat tatctcgaca gggatcctgg caaggcgctg tcgaaggctt
     1441 acgacatggt gctcaacggc tgggagatcg gcggcggatc ggtgcgtatc catcgccagg
     1501 acgtgcaatc caaggtattc agggcgctgg gcatcggtgc ggaagaggcg cagaaaaaat
     1561 tcggtttctt gctggaagcg ctgcagttcg gcgcgccgcc ccacggcgga ctggccttcg
     1621 gcctggaccg gatcgtcacc atgatggcgg gcgtggaatc catccgtgac gtgatcgcct
     1681 tccccaagac ccagcgggcg caggacctga tgtgcaaggc gcccggcccc gtggacgaga
     1741 agcagctgcg cgagctgcat atacgcctgc gcaacgtgga accggtgaag gcgaactaac
     1801 cacagagaca cagaggcact gagaagaact caatacgggg ctgagaaacc tcacgtactc
     1861 gccgatgaat gctttgcgtg ggccgtgctt gggcgttctc gttcaagtaa tgcttatttg
     1921 tttctcgact ctctctgtgt ccccgtgtct ctgtggtgaa ggtaattgaa gttcaagatt
     1981 ccgatttcgg tgctggtggt gatccacacc cccgacctgc aggtgctgct gctggagcgc
     2041 gcggatcacc cgggcttctg gcagtcggtc accggcagcc aggacgaggg cgagacgctg
     2101 cgccagaccg ctgcgcggga agtggcggaa gagaccggcc tggatgccga g
//
LOCUS       DASQOP010000349         4291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_986992, whole genome shotgun sequence.
ACCESSION   DASQOP010000349 DASQOP010000000
VERSION     DASQOP010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986992"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1131)
                     /gene="glp"
                     /locus_tag="VE030_13540"
     CDS             complement(<1..1131)
                     /gene="glp"
                     /locus_tag="VE030_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HZF24476.1"
                     /translation="MDSAKSLHELSTASDYDPNSMPVEKARSHIRAFLEPVTAVERLN
                     IRAALGRVLAEEVISPVNVPQHDNSAMDGYAVRFDDLKKDGEATLKVIGTAFAGKPFD
                     GSVGPGQAVRIMTGAVIPQGTDTVIQQERAKTSGDTVVVAPVTKKGTNTRSAGEDLRQ
                     GEPALKRGQPLRPAEIGLLASLGIGEVPVYRKLRVVFFSTGDELVPVGRSLAAGQIYD
                     SNRYTLYGMLTRLGCDVLDMGVIRDDPKAVERVFDEAAQSADVVITSGGVSVGEADYV
                     KQILERLGEVLFWKIAMKPGRPLAYGRIGRAHFFGLPGNPVAVMVTFYQFVRDALLYL
                     QGQATVAPLPTLKVTCTSAIKKAPGRTEFQRGVLSRAADGQWT"
     gene            complement(1363..2415)
                     /gene="moaA"
                     /locus_tag="VE030_13545"
     CDS             complement(1363..2415)
                     /gene="moaA"
                     /locus_tag="VE030_13545"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HZF24477.1"
                     /translation="MNEPFISSEQLPAGHGRPVADTRGRPLRDLRISVTDRCNFRCTY
                     CMPKEVYGRDFQFLERSQLLTFDEIHRLVRIFRGHGIEKVRITGGEPLVRRQLEKLIE
                     LLAQEGDLDLTLTTNGSLLKQKARALKDAGLKRVTVSLDSLDDAVFRVMNDVDFPVAK
                     VLEGIEEADKAGLAPLKVNMVVKRGTNDQSVVDMAHFFKGSGHILRFIEFMDVGHTNG
                     WKMEHVVPSRELVARINALFPIEPMDANYPGEVAERWRYKDGSGEIGFISSVTHAFCK
                     DCTRARLSAEGSIYTCLFATQGTDLRSLIRGGASDEEISAAIAKVWRARTDRYSEIRT
                     GETAKLHKVEMSYIGG"
     gene            2579..3367
                     /locus_tag="VE030_13550"
     CDS             2579..3367
                     /locus_tag="VE030_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZF24478.1"
                     /translation="MLGCLLLLFCGIAGAHAAPDLRLATTTSTENSGLLNAILPLFEA
                     KYGTRVRVISVGSGKAMKLGENGDVDVVLVHSRADEEKFVATGFGVNRRDVMYNDFVI
                     VGPKSDPAGIRGSDVLKAFEKIAAAGSRFVSRGDDSGTHKMEQKYWKATGVEPAAMSG
                     YVSAGLGMGEVLMMAAEMRCYTLADRATFAAYRAKTGLEILVSGDPRLFNPYGVIAVN
                     PARHPDVNHAGAMQFIGWLTSAEGQAAIAAFRVDGEQLFFPNGR"
     gene            3368..4078
                     /locus_tag="VE030_13555"
     CDS             3368..4078
                     /locus_tag="VE030_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZF24479.1"
                     /translation="MDLLDTSREAFRLLFSGDPTLWGIVWVSIKVSVLALILAGPLAV
                     AVGFVLAHVAFPGRRLLVILVQAFQSFPTVVVGLLLYLLLSRQGPLGALHLLFTQPAM
                     IFGQMVIAFPVLAAYTLTAVQGADPRAHETAASLGAGRVRAAMTTLWEVRFGVMAAVF
                     MGFGRIISEIGCSLMVGGNIAGLTRNIPTAIALETSKGEFAQGVALGIVLIVLALAVN
                     VALAAVQGQGGMRQAWQP"
     gene            4066..>4291
                     /locus_tag="VE030_13560"
     CDS             4066..>4291
                     /locus_tag="VE030_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZF24480.1"
                     /translation="MAAVIEVTALAKTWGGRPLLRIPQLVLEAGSSYVLSGDNGAGKS
                     TLLRILAGLEPAQVERFAFEGNLLQLDRLPA"
BASE COUNT          754 a         1280 c         1381 g          876 t
ORIGIN      
        1 cgtccattgc ccgtccgcag cgcggctgag gacgccgcgc tggaattcgg tgcgcccggg
       61 cgctttcttg atcgcggacg tgcacgtcac cttgagggtc ggcagcggcg caaccgtggc
      121 ctgtccctgc aggtagagca gggcatcgcg cacgaactgg tagaaggtca ccatcaccgc
      181 aaccggattg ccgggcagcc cgaaaaaatg cgcgcgcccg atcctcccat aggcaagcgg
      241 ccggcccggt ttcatcgcga tcttccagaa cagaacttcg cccaggcgct cgaggatctg
      301 tttgacgtaa tcggcttcgc ccacggatac cccgccgctg gtgatcacca cgtcggctga
      361 ttgtgcggct tcgtcaaaga cacgctccac cgccttcgga tcatcgcgga tcacacccat
      421 gtccagcaca tcgcaaccca gccgggtgag catgccgtag agggtatagc ggttgctgtc
      481 gtagatctgc ccggcagcga gcgacctgcc gaccggaacc agttcgtcgc cggtggagaa
      541 aaacacaacg cggagtttcc gatataccgg gacttcgccg attccgagcg aagcgagcaa
      601 tccgatttcc gcggggcgaa gcggctgccc gcgcttcaaa gccggttcgc cctggcgcag
      661 atcctctccc gcgctgcgcg tgttggtgcc tttcttcgtc accggcgcga ccaccaccgt
      721 atcgccggat gtttttgcgc gttcctgctg aataacggtg tccgttccct ggggaatcac
      781 cgcgccggtc atgatgcgta ccgcctgccc ggggccgacg ctgccgtcga atggcttgcc
      841 ggcgaaagct gtgccgatca ccttgagcgt cgcctcgccg tccttcttga gatcgtcgaa
      901 tctgacggca tagccatcca tcgccgaatt gtcgtgctgg ggcacattga ccggcgaaat
      961 gacctcctcg gcgagcacgc gcccgagtgc ggcgcggata ttcaggcgtt cgaccgctgt
     1021 gaccggttcc agaaaggcac gaatatggct gcgtgctttc tccaccggca tcgagtttgg
     1081 gtcgtagtcg gatgccgtgc tcagttcgtg cagggatttt gcggaatcca tgggtaatct
     1141 tcggcttggg gcaaggagag agacgttgga ggctagaggc gagaggcaag aggctagagg
     1201 aaagaggaaa tccccacact tgccgacccg ttatgcagca tcgccacgtc gcaaaagaat
     1261 tgcgagtcaa aagtccaatg cttctcgtag atttgcatat cctctttata tctgcgcctt
     1321 ggcttgcggc tttgtcttcc tctcgcctct agcctcttgc ctctagcctc caatatatga
     1381 catctcgacc ttgtgcagct tggcggtctc gccggtgcgg atttcggaat aacggtcggt
     1441 acgcgcgcgc cagaccttcg cgatggccgc ggatatttcc tcgtccgagg caccgccccg
     1501 gatcaggctg cgcaggtcgg tgccttgcgt ggcgaacagg caggtataga tcgaaccttc
     1561 cgctgacagc cgcgcgcgcg tgcagtcctt gcagaacgcg tgggtaacgg aggagatgaa
     1621 gccgatttca ccggagccgt ccttatagcg ccagcgctcg gcgacttcgc cggggtaatt
     1681 ggcgtccatg ggctcgatag ggaataacgc attgatcctc gcgaccagtt cgcgcgaagg
     1741 cacgacgtgt tccattttcc agccgttggt atggccgaca tccatgaatt cgatgaaacg
     1801 cagaatatgg ccgctgccct tgaaaaagtg cgccatatcc acgacgcttt ggtcgttggt
     1861 cccgcgcttg accaccatat tgaccttgag cggcgcgagg ccggccttgt cggcttcctc
     1921 gatgccttcc agcactttcg ccaccgggaa gtccacgtcg ttcataaccc ggaacaccgc
     1981 atcgtccaga gaatccaggc tgacggtgac gcgcttgagt cctgcgtcct tcagcgcccg
     2041 ggctttctgc ttcagcaggg aaccgttggt cgtgagcgtg aggtcgagat cgccctcctg
     2101 cgccagcagt tcgatcagtt tctcgagctg gcgacgtacc agcggctcgc cgccggtaat
     2161 gcgcaccttc tcgatgccgt ggccgcggaa aatcctcacc agccggtgaa tctcgtcgaa
     2221 ggtgagcaac tgcgaacgct ccaggaactg gaaatcccgt ccgtacacct ccttgggcat
     2281 gcagtaggtg cagcggaagt tgcagcggtc ggtcaccgaa atccgcagat cgcgaagagg
     2341 ccggccgcgg gtgtcggcga caggacgccc atggccggca ggcagttgct cggaactgat
     2401 gaagggttcg ttcatgggtg cttgttgtaa tgtgtgatgg tgcacgccat cgacagcgcg
     2461 gccggccgta atgtgaggca ttatattgca aaagatataa cgccagctaa agtcttggcg
     2521 gtgggcattg tcctgatctg gccggggagt tgcgatcttg actgcattat tttggagaat
     2581 cctgggctgc ctgctgctgt tgttctgcgg catcgcgggc gctcacgccg ccccggactt
     2641 gcgactggcc accaccacca gcaccgagaa ttcgggcctg ctgaacgcga tcctgccttt
     2701 gttcgaagcg aaatacggca ccagagtccg cgtcatctcg gtagggtcgg gcaaggcgat
     2761 gaagctgggc gagaacggcg acgtggacgt ggtgctggtg catagccgcg ccgacgagga
     2821 aaaattcgtg gccacggggt tcggggtcaa ccgccgcgac gtgatgtaca acgacttcgt
     2881 catcgtgggc ccgaaatccg acccggcggg catacgtggc agcgacgtac tcaaggcgtt
     2941 cgaaaagatt gcggcagcgg gcagccgctt tgtgtcacgc ggcgacgatt cgggtacgca
     3001 caaaatggag cagaaatact ggaaggcaac cggtgtcgag cccgcggcga tgtccggtta
     3061 tgtttcggcg ggactgggaa tgggcgaagt cctgatgatg gcggcggaga tgcggtgcta
     3121 caccctcgcc gaccgcgcta catttgctgc gtaccgggcg aagacgggat tggaaatcct
     3181 ggtgtcgggc gacccgagac tcttcaaccc gtacggggtc attgccgtga atccggcgcg
     3241 gcatccggac gtcaatcacg ccggcgcgat gcaattcatc gggtggctga cttcagctga
     3301 aggacaggct gcgatcgccg ctttccgggt ggatggcgaa caactgttct tccccaacgg
     3361 gcgctgaatg gaccttctcg atacgagccg ggaggcgttc cggctgctgt tctcgggcga
     3421 cccgacgttg tggggaatcg tctgggtatc gatcaaagtc tcggtcctgg cgctgattct
     3481 ggccgggccg ctggcggtgg cggttggttt cgtattggcg cacgtcgcct ttcccgggag
     3541 acggctgctg gtgatcctgg tgcaggcgtt ccagtccttt ccgacggtcg tcgtgggtct
     3601 gttgctctac cttctgttgt ccaggcaagg gccgctaggc gcgttgcatc tgctcttcac
     3661 tcagcctgcg atgatcttcg gccagatggt cattgccttt ccggtcctcg ctgcatacac
     3721 gctgactgct gttcagggcg ccgatccgcg tgcccatgaa accgcggcca gcctcggcgc
     3781 cggccgcgtg cgcgctgcga tgacgacctt gtgggaagtg cgcttcgggg tgatggccgc
     3841 ggttttcatg ggtttcggcc ggataatctc ggagatcggc tgttcgctga tggtcggcgg
     3901 caacatcgcc gggctcacgc gcaacattcc cacggcgatt gcgctggaaa ccagcaaggg
     3961 cgagttcgcc cagggcgtgg cgttgggaat cgtactgatc gtgctggcgc tggcggtgaa
     4021 cgtcgcgctg gcggcggtgc aggggcaggg cggaatgagg caggcatggc agccgtgatc
     4081 gaagtcaccg cgctcgccaa gacctggggc ggccgtccgc tactgcgcat tccgcagctt
     4141 gttctggaag cgggctcgag ctacgttctt tccggcgaca acggagcggg gaagagcacg
     4201 ttgctgcgca ttctcgccgg gctggagccg gcgcaggtgg agcgctttgc gttcgaaggc
     4261 aatctcttgc agctcgaccg cttgcccgcg c
//
LOCUS       DASQOP010000350         6926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2997368, whole genome shotgun sequence.
ACCESSION   DASQOP010000350 DASQOP010000000
VERSION     DASQOP010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6926
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2997368"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            351..890
                     /locus_tag="VE030_13565"
     CDS             351..890
                     /locus_tag="VE030_13565"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HZF24481.1"
                     /translation="MTTVPEQISAFGKAQVETALRFVTIAAEGTEKLFDLQIKNSKAA
                     FNEGMKNAKALAEVKDFSELPTWTSSTFQPGVDKATAYARSLYEVAAGTQSEISAVLE
                     AQIADFSKDVAVALDAALKSAPAGSESAVAAVKSVIGTANTLYESIAKANKQLAAMTE
                     ANFTAAASQAGVATKKKAA"
     gene            complement(937..1731)
                     /locus_tag="VE030_13570"
     CDS             complement(937..1731)
                     /locus_tag="VE030_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24482.1"
                     /translation="MKPMSPEDQELEELRSDLSGRYRAGSTEEPRAHLDAAIFAAARR
                     EVARSRFTRNWHLPASIAAVLVIGVSLALMTSEIEDPLPPVDKSAGVAADKAKQAAPA
                     LAMKQEPQARPAPRPGIERESRPSRERSEGTDREADTRQNLAAERADSVAAAPSVAAS
                     TSAPASPPLEAAATVEPVAEQKIALADPAREKRATGALRKEATPSPAAPADAPGEWLK
                     RIGDLLRNGKTAQAREQLAEFRKHYPDYRLPETLRELEVSVKPIPQ"
     gene            complement(1712..2320)
                     /locus_tag="VE030_13575"
     CDS             complement(1712..2320)
                     /locus_tag="VE030_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZF24483.1"
                     /translation="MITLQAGMDQPASDEDLMLRYRDGDVRAFEDLYARHRGAVFRYV
                     LRQVGFRSAAEEVYQEVWTNIISSRARYRVEARFATFLYRVAHNCVIDHFRRKPPLQL
                     ISLDDDAEEAMQVAGPRHEQPERAVARRQTARQLLRALALLPDEQREAFLLHEEGGLT
                     LEEIADVTGVGRETVKSRLRYALVKLRQELNGSGMSHEADVA"
     gene            complement(2360..3103)
                     /locus_tag="VE030_13580"
     CDS             complement(2360..3103)
                     /locus_tag="VE030_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24484.1"
                     /translation="MRRFLPFLCLFLTVAPTLAAGNIAEVQIYDRAGGRTLPVYESGG
                     RWFVAGKPGNEYQVTVRNCAGGDLLAVVSVDGVNVVTGETAAASQSGYVVERGRTLSV
                     KGWRKSLEKVAAFYFTDLGDSYAARTGHPDNVGVIGVALFKRKPEPVLELEQPQNRGE
                     PGAAADGAARSAAPRAVEKSIGTGHGRSEPSQARYAAFERESDTPNEIVVIHYDTYAN
                     LVARGVIPQSPRWPAPFPGGFVPDPPGRG"
     gene            complement(3278..4018)
                     /locus_tag="VE030_13585"
     CDS             complement(3278..4018)
                     /locus_tag="VE030_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HZF24485.1"
                     /translation="MFGALIIGDEILSGKRQDKHFPKIVSILKARGLSLGWSMYLPDE
                     PEVIAAVLSSTLARDDIVFCFGGIGATPDDHTRQCAAKAAGVELVLHPDAKREIEARF
                     GAEAYPKRIEMGVFPAGSRIIPNPYNRVPGFSYARHHFFPGFPEMAWPMMEWVLDNEY
                     PHLHNQRPPVDVAIVVKDAGESQLIDLMNACIARYPGVKLFSLPSFTPAGRRIELGVK
                     GEAKLASEALAFLQKGVSALGFAWEPRN"
     gene            complement(4086..4340)
                     /locus_tag="VE030_13590"
     CDS             complement(4086..4340)
                     /locus_tag="VE030_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24486.1"
                     /translation="MFKRMLLIVALAGAVAGCKTQAALTAGATGCRETDVDVIASRYS
                     REGTTTAWCAKCKDQIYHCVSNPERSRVECRPADSSDRCG"
     gene            complement(4409..4837)
                     /locus_tag="VE030_13595"
     CDS             complement(4409..4837)
                     /locus_tag="VE030_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZF24487.1"
                     /translation="MKTVQQLLDSKRHRLLSVSPDTTVLDALKLMAEKEIGAVLVLEG
                     DSLAGIFSERDYARKVVLQGKSSKDTPVREIMTDKVVCVRPEQSIEDCMGLMTDKRVR
                     HLPVLEHKKVVGVISIGDVVKEMLSEQEFVIKQLESYIHN"
     gene            complement(4942..6525)
                     /locus_tag="VE030_13600"
     CDS             complement(4942..6525)
                     /locus_tag="VE030_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta
                     family protein"
                     /protein_id="HZF24488.1"
                     /translation="MKPTANAQRPITLLICALGGEGGGVLAEWLVEAASQCGYSAQST
                     SIPGVAQRTGSTTYYFEVFPVPIAELAGRKPVFSLYPVPGALDVLVSSELLETVRQIG
                     NGMASADRTLVVSSSARTLTTAEKLQLADGRAPSGELLNVVNRYSRDARILDMAAVAQ
                     ETGTVLSAVLFGAIAGSGALPFPRETFERTIRKSGKGVDASVRGFERAYEIVAQGQPA
                     PILAGSKARTRVAMPAELAREFPAATHEMLAVGYARMLEYQDHDYAQLYLRRLARILA
                     AERAVDPAGLGNFAITSETARYLALWMAFDDIVRVADLKCRASRYKRVRAEVKAAPEE
                     VLRIYDYFKPGVPEFAALLPAGFAAALIRWDRRRQQAGKSALALPLKLATHTLSGFLI
                     LRTLASLKWLRKRGSRFAIEQSTIERWLAGTEQGTRSHWQLGHEIAQCGRLIKGYGTT
                     NERGKENLLHVLDHLAAAASFPSAAARAEAIRAARVAALADEAGKAFDRTLVQHGAPA
                     RPVKAQPVMWVKKRPGQAA"
     gene            complement(6551..>6926)
                     /locus_tag="VE030_13605"
     CDS             complement(6551..>6926)
                     /locus_tag="VE030_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase"
                     /protein_id="HZF24489.1"
                     /translation="AEGECQLERQRRIKPWIAGLLKAGKRVVRVKYGVDEDVCTGDHA
                     CIRLSGCPTLTLKDSPDPLRVDPVATVIDGCVGCGLCGENAHAATLCPSFYRAEVIRN
                     PRLYERVIDGLRGSVIRLLQTA"
BASE COUNT         1248 a         2272 c         2130 g         1276 t
ORIGIN      
        1 gtcgcgaacc caaaggcacg cgacgcggat acgaaggcca ttcgcgattt caaccggcat
       61 ctccgggacg accgacgggt gcatctgtgt ttggtcccta tcggggatgg cctcagcctg
      121 gccctcaagc tctaatttaa atttagtata aattttcaaa cggttatgtc agaaacgaca
      181 gcttcgtgcg tcggtttatt gtgcgcagca ataaatttcg tcgcatttgt cctctgaatt
      241 gcttgacatc ggttgtttcc gtgccaataa tgtatttgct gcgatgcaac atgacgcctc
      301 ccgctgctga cgcgtaatat cttatcaact gtttttaaag gagtcccaga atgacgaccg
      361 tacccgaaca gatctcagcc tttggcaagg cgcaagtgga aaccgccctg cgcttcgtaa
      421 ccatagcggc tgagggcacc gaaaagttgt tcgacctgca gatcaagaat agcaaggccg
      481 ccttcaacga aggcatgaag aacgccaagg cacttgccga agtgaaggat ttctccgaac
      541 tgccgacttg gaccagctcg acattccagc cgggcgtcga caaggccacc gcctacgccc
      601 gcagcctgta tgaagttgct gccggtaccc aatccgaaat cagcgcggtg ctggaagcgc
      661 agatcgccga tttcagcaag gacgtagccg ttgctctgga cgcggcgctg aagtccgccc
      721 ccgcgggttc cgagtccgcg gtggccgcgg tcaagtccgt gatcggcact gcgaacaccc
      781 tgtacgaaag cattgccaag gcgaacaagc agctggccgc gatgaccgaa gccaacttca
      841 ccgccgccgc ctcgcaagcg ggcgtggcga ccaagaagaa ggctgcgtaa gtttgcctca
      901 ccgaaagagc gaaagccccg gacatccggg gcttttttat tgcgggattg gcttcaccga
      961 gacctccagc tcacgaagcg tttccggcag gcggtagtcc gggtagtgct tcctgaattc
     1021 cgccagttgc tcgcgcgctt gtgcggtttt gccgttgcgc agcagatccc cgattctctt
     1081 cagccattcc ccgggcgcgt cggctggcgc cgcgggggat ggcgtcgcct ccttgcgcag
     1141 tgccccggtg gcgcgctttt cccgcgctgg atccgcaagc gcaatcttct gctccgctac
     1201 cggttccacc gtggcggcag cttcgagcgg cggtgacgcc ggcgcagatg tcgatgccgc
     1261 caccgaaggc gccgccgcaa ccgaatcggc acgttcggca gccaaatttt gccgggtgtc
     1321 cgcctcacgg tcggtgcctt cactgcgttc gcgcgacggc cgcgattcgc gctcgatgcc
     1381 agggcgcggc gcgggccgtg cctgcggctc ctgcttcatc gccagcgcgg gcgcagcctg
     1441 cttggcctta tccgccgcaa cgccggccga tttatctact ggcggcaatg gatcctcgat
     1501 ttcgctggtc atcaacgcca ggctcacgcc gatcaccaac acggccgcaa tcgacgcagg
     1561 caaatgccag ttgcgcgtga aacgggagcg cgccacctca cggcgggcgg ctgcgaagat
     1621 cgcggcgtcc agatgcgcac gcggctcttc ggtcgatccg gcacggtagc gcccggacag
     1681 gtccgaacgc agctcctcca gttcctgatc ttcaggcgac atcggcttca tggctcattc
     1741 cagacccatt caattcctgg cgcagcttga caagcgcgta cctgaggcgg cttttcaccg
     1801 tctcccggcc gaccccggtg acgtcggcga tttcctccag cgtcaagcca ccctcctcgt
     1861 gcagcagaaa agcctctcgc tgctcgtccg gcagcagcgc cagcgcccgc agcagctgcc
     1921 ttgccgtctg ccgcctcgcc acggcgcgct ccggctgttc gtgccgcgga cctgccacct
     1981 gcatcgcctc ttccgcatcg tcgtcgagag aaatcaactg caacggcggt ttgcggcgaa
     2041 aatggtcgat cacgcaattg tgcgccacgc gatacaggaa cgtggcaaag cgggcttcga
     2101 cacggtagcg ggcacgcgag gaaatgatat tcgtccacac ttcctggtac acctcctcgg
     2161 cggcagagcg aaagccgacc tgccgcagca cgtagcggaa cacggcgccg cgatggcgcg
     2221 cgtacaaatc ctcgaacgcg cgcacatcgc catcgcggta acgcagcatc agatcttcgt
     2281 cggaagccgg ctggtccatt ccggcttgaa gtgtaatcaa cctcagcacg acgcgcgata
     2341 ccgcgggcat tccaatgcct caaccccgcc ccggcggatc gggaacgaac ccgcccggaa
     2401 acggcgcggg ccaacgcggc gactgcggaa tgactccgcg tgccaccaga ttcgcgtagg
     2461 tgtcgtagtg aatgacgacg atttcgttgg gtgtgtccga ttcgcgctcg aacgcggcat
     2521 aacgtgcctg cgaaggctcg ctgcggccat ggccggtgcc gatgctcttc tccaccgcac
     2581 ggggtgccgc cgaccgcgcg gcgccatccg ctgcggcgcc gggttcgccg cggttttgcg
     2641 gctgctccag ttcgagcacg ggttcaggtt tgcgcttgaa tagggccacg ccgatcacgc
     2701 cgacgttgtc cgggtgcccg gtgcgcgcgg cgtaggaatc gcccagatcg gtgaagtaga
     2761 atgcagccac tttttccaga ctcttgcgcc aacccttgac cgacagcgtg cggccgcgct
     2821 caaccacgta gccgctctgc gacgcggcgg cagtctcgcc agtgacgaca ttgacgccgt
     2881 ctaccgacac caccgcgagc agatcacccc ctgcgcagtt gcgaaccgtg acctggtact
     2941 cgttgccggg tttgccggcg acgaaccagc gaccgccgct ctcgtagacc ggcagcgtgc
     3001 ggccgccggc gcgatcgtag atctgcacct cggcgatgtt gccggccgcc agagttgggg
     3061 cgaccgtcag gaaaagacac aaaaacggca ggaagcgtcg catggtgttc tccattgaag
     3121 agggacaccg gctataaacg gtcgcttcgg cggaatgggg ttaaatgtcg gggaagaggg
     3181 gcgagggtcg agggtcgagg ggtgagtaag aacgtcacac agtcatttag agccagtcga
     3241 gggctttcgc cctcgcccct cgcccctcgc ctctcgccta gtttctcggc tcccaggcaa
     3301 agccgagcgc gctgacaccc ttctgcagga acgccagcgc ctcactggcg agttttgctt
     3361 cgcctttcac gccgagttcg atgcgccggc cggcgggcgt gaaactgggc aggctgaaaa
     3421 gtttcacgcc gggatagcgg gcgatgcagg cattcatcag atcgatcagc tggctctccc
     3481 cggcgtcctt caccacgatc gccacatcca ccggcggtcg ctggttatgg aggtgcgggt
     3541 actcgttgtc gagcacccac tccatcatcg gccaagccat ctccgggaac ccggggaaaa
     3601 aatggtggcg cgcataactg aaaccgggaa cacggttgta ggggttggga atgatgcggc
     3661 tgccggccgg gaacacaccc atctcgatgc gcttgggata ggcctcggcg ccgaagcgcg
     3721 cctcgatttc gcgcttggca tccggatgca acacgagctc gacgccagcg gctttggccg
     3781 cgcactggcg ggtgtggtcg tcgggcgtgg caccgatgcc gccaaaacag aatacgatgt
     3841 cgtcgcgcgc cagggtgctg gacagcaccg cggcgatcac ttcgggctcg tccggcaaat
     3901 acatgctcca gcccaggctc aggccgcgcg ccttcagaat cgagacgatc ttcggaaaat
     3961 gcttgtcctg gcgtttgccc gagaggattt catcaccgat gatcaaggca ccgaacatga
     4021 ggctgaccaa tcatggctga cgagtggttg gaatccgtcg gaatgccgca acgatactgg
     4081 catcatcagc cgcaccgatc cgaggaatcg gccggcctgc actcgacgcg gctgcgctcc
     4141 ggattggaca cgcagtgata gatctggtcc ttgcacttgg cgcaccatgc cgtggttgtg
     4201 ccctctctgg agtaccggct ggcgatgacg tccacatcgg tctcgcggca gcccgttgct
     4261 ccggcggtga gcgcggcctg ggtcttgcag cccgcaaccg cgcccgcgag cgcaacgatc
     4321 agaagcattc gtttgaacat gagatcgaaa taccggaagt gagggccgcg aagaccggag
     4381 ctgcacgcag cgggccacgg tgacaacttc agttgtggat gtagctttcg agctgcttga
     4441 tgacgaactc ctgttcggag agcatttcct tgaccacgtc gccgatggat atcacgccga
     4501 ccaccttctt gtgctcgagc acgggcagat ggcgtacgcg cttgtcggtc atcagcccca
     4561 tgcaatcctc gatgctctgc tccgggcgta cgcacaccac cttgtcggtc atgatctcgc
     4621 gcaccggcgt gtccttcgac gacttgcctt gcagaaccac cttgcgcgcg taatcgcgtt
     4681 cggaaaaaat tcccgccaga ctgtcgccct ctagcaccag aaccgcgccg atctccttct
     4741 ccgccatcag cttgagcgcg tccagcaccg tggtatcggg cgacaccgag agcaggcgat
     4801 ggcgtttcga atccagcagt tgttgaactg ttttcatgtt ttcctccgcg ctgaagcagc
     4861 cgcctggccg gtgattttca gcccagttta gaccacaagc cgcacttgtc aacgccgggc
     4921 gccggcacgc aggcttcgcg ctcaggccgc ctggcccggc cgtttcttca cccacatgac
     4981 gggctgggcc ttgaccgggc gcgccggcgc gccatgctgg accagcgtgc ggtcgaacgc
     5041 tttgcccgcc tcgtcggcca gcgccgccac gcgcgccgcc cgaatcgcct cggcacgcgc
     5101 cgcggcggac gggaaagacg cggcagcggc cagatggtcg agaacgtgca acagattctc
     5161 cttgccgcgt tcgttggtgg tgccgtagcc cttgatgaga cggccgcact gggcaatttc
     5221 gtggccgagc tgccagtggc tgcgagtacc ctgttccgtg cccgccagcc agcgctcgat
     5281 cgtggactgc tcgattgcga agcggctgcc gcgcttgcgc aaccacttga ggctggccag
     5341 ggtgcgcagt atcaggaaac cggacagcgt gtgcgtagca agcttgagcg gaagcgcgag
     5401 cgcggacttg cccgcttgct ggcggcgccg gtcccagcga atcagcgcag ccgcgaaccc
     5461 ggccggtaac agcgcggcaa attccggtac ccccggcttg aagtagtcat agatgcgcag
     5521 tacttcctcc ggcgcggcct tgacctcggc gcgcacccgc ttgtatctgc tggcgcggca
     5581 cttcagatcg gccacgcgca cgatgtcgtc gaaggccatc cacaatgcca ggtaacgcgc
     5641 cgtctcgctg gtgatcgcaa agttccccag gccggctgga tcgaccgccc gctcggcagc
     5701 cagaatgcgg gccagccgcc gcaggtagag ttgcgcgtag tcgtggtcct ggtactccag
     5761 catgcgggcg tagccgacgg ccagcatctc atgggtcgct gcgggaaact cgcgggcgag
     5821 ttctgcaggc atcgcgactc ttgtccgcgc cttcgagcca gccagtatgg gggcaggctg
     5881 tccctgggcc acgatttcgt aggcgcgctc gaaaccgcgc acgctcgcgt ccacgccttt
     5941 gccggatttc cgtatggtcc gctcgaaggt ctcgcgcgga aacggcaatg cgccgcttcc
     6001 cgcgatcgcg ccgaacagaa ctgcgctcaa cacggtgccc gtctcctgcg ccaccgccgc
     6061 catgtcgaga atgcgggcgt cgcggctgta acggttcacc acattcaaca gttccccgga
     6121 cggcgcgcga ccatccgcaa gctggagctt ctcggccgtg gtcagggtcc gggcgctgga
     6181 gctgacgacc agcgttcgat cggcggacgc catgccgtta ccgatctggc gcacggtttc
     6241 cagcagttcc gaggacacca gcacgtccag cgcgcccggt accggataga ggctgaacac
     6301 aggcttgcgg ccggccagtt ccgcgatcgg caccggaaat acttcgaagt aataagtggt
     6361 cgaaccggtg cgttgggcga caccgggaat cgaagtgctc tgcgcgctgt agccgcactg
     6421 actggccgct tccaccagcc attcggccag cacgccgcca ccctcgccgc cgagcgcgca
     6481 gatcaacagc gtaatcggac gttgggcatt ggctgtcggc ttcatcgtgc ctctgtgtct
     6541 ctgtggtaag tcatgctgtt tgcaacaacc tgataactga accgcgcagt ccgtcgatga
     6601 cgcgttcgta cagcctggga ttgcgtatca cttcggcccg gtagaacgac gggcacagcg
     6661 tcgccgcgtg cgcattttcg ccgcacaggc cgcagcccac gcaaccgtcg atcaccgttg
     6721 ccaccggatc gaccctgagt ggatcggggc tgtctttaag tgtcaacgtc gggcagccag
     6781 acagccggat gcaggcgtga tcgcccgtgc agacatcctc gtcgacgccg tacttcaccc
     6841 gcaccacgcg ttttcccgcc ttgagcaggc cagcgatcca gggcttgatg cggcgttgcc
     6901 gttcgagctg gcattcgcct tcggcg
//
LOCUS       DASQOP010000351         4434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1191913, whole genome shotgun sequence.
ACCESSION   DASQOP010000351 DASQOP010000000
VERSION     DASQOP010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191913"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..117)
                     /locus_tag="VE030_13610"
     CDS             complement(<1..117)
                     /locus_tag="VE030_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HZF24490.1"
                     /translation="MADQMKIRAQLHGDVADVKILIFHPMETGLRKDGVTGQL"
     gene            complement(146..589)
                     /gene="soxY"
                     /locus_tag="VE030_13615"
     CDS             complement(146..589)
                     /gene="soxY"
                     /locus_tag="VE030_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003273928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HZF24491.1"
                     /translation="MRRSVLRAAAVAMATAILRPLQLLAGQWNKAAFDAKVVTDALKG
                     IGVTAMEDSDRILLKAPEIAENGAIVPLEIASQIPGTQAIYILVDKNPQPLTAVFEFA
                     NGAEAFVATRIKMNESSMVRILARAGGKFYATMREVKVTIGGCGA"
     gene            complement(625..1050)
                     /gene="soxX"
                     /locus_tag="VE030_13620"
     CDS             complement(625..1050)
                     /gene="soxX"
                     /locus_tag="VE030_13620"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HZF24492.1"
                     /translation="MSLQEPARAGSVSALEHGRSPLYRRLIVVVLIGTAFFPSISQTR
                     ADEAPNVAAGRRLAFEIAKGNCPACHMFPGDPGADTLANIGPPLLAIRSRFPDRAQLR
                     GRLWDPMQANPNTVMPPFGKHRILSSDEIELIIDYLYTQ"
     gene            complement(1047..1535)
                     /gene="moaC"
                     /locus_tag="VE030_13625"
     CDS             complement(1047..1535)
                     /gene="moaC"
                     /locus_tag="VE030_13625"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010101882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HZF24493.1"
                     /translation="MGELTHFDSRGQAHMVDVADKQETRRVAIASGRIFMRADTLRKI
                     VEGDAAKGDVLGVARIAAIQGSKRTSELIPLCHPVALTKVSVDFEVHRDDNSVECIAV
                     AETVGRTGVEMEALTAVSVGLLTVYDMCKAVDRGMRIDGVGLREKRGGKSGHWVAGTS
                     SG"
     gene            1612..3039
                     /locus_tag="VE030_13630"
     CDS             1612..3039
                     /locus_tag="VE030_13630"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HZF24494.1"
                     /translation="MKFRSVIAMVLLVPALGLGDELPDLGDVSQGALSPAQERKLGEG
                     IMSQIRASPVYIDDPDISDYLNAVGYRLVGNSPDPAGPFEFFGIAENSVNAFALPGGF
                     VGVHSGLLLIAQSESELASVLSHEIAHVTQRHLARMVAAQQRAGIASMAALAVAILAA
                     RSNPEVSAAAITAAQAGTIQSALNFTREHEREADRIGLQIMEKSGFDVHAMPVFFERL
                     QKATRVYETGAPAYLRTHPLTFERIADVQSRIEGVPFRQVPDSLEFHLVRARLSALEG
                     NPRVAVTNFDDMLSGRKFTSEAAARYGLVVALLRAGDFARARKELAPLQSLKAKSPMI
                     DALAAQTLIGSGELEKGLATYRDGLRANPSRRALVHGYANALLDATRAAEAVDFLSGR
                     LETRTPDARLYELQSRGYTALGKHLLQHRAQAEAYVLRGNLTAAIEQLHLAQRAGDGD
                     FYQKSSVEARLKELVAIDTEARRRP"
     gene            3215..>4434
                     /locus_tag="VE030_13635"
     CDS             3215..>4434
                     /locus_tag="VE030_13635"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZF24495.1"
                     /translation="MEKIGKYEIIRELGRGATSAVYLANDAFANRQVAIKLVRSDALG
                     DKDHGRRFHKLFLTEASLAGKLSHPHIVSIFDAVVDEQGSYLVMEYVEGGTLEPLTQP
                     ESLLPVSRVIEIIFKCCKALDYAHRHGIIHRDIKPANILLTGESDIKISDFGAALTIA
                     AETTQVSGIGSPAYMSPEQLQEFVLNHQTDIFSLGVVMHQMLTGALPFKATNNYSMVY
                     QIIHVDAPPPSMLRPEIPAAVDAIVRRALEKETAKRFQTWEEFAQALADSFNNLQKPE
                     EVVPETEKFNTLRRLEFFRTFSDVDLWQVLRISNWARYSANSLVIKEGDVGTSFYVVA
                     AGEVKITKQGKLLNILRTGECFGEMAYLGKRLFQRTASVASVNDITIVEVGADSLAQA
                     TESCKHAFNAAFLEL"
BASE COUNT          827 a         1447 c         1314 g          846 t
ORIGIN      
        1 cagctggccg gtgacgccgt ccttgcgcag gccggtctcc atcggatgga atatcagtat
       61 tttcacgtca gccacgtcgc cgtgcagctg cgcgcgaatt ttcatctgat cggccatctg
      121 gcgctcccct ttcctgttcc cgtactcagg cgccgcagcc gccgatcgtg accttgactt
      181 cacgcatggt ggcgtagaac ttgccgcccg ccctggccag gatccgtacc atcgacgatt
      241 cgttcatctt tatccgggtg gccacgaatg cttccgcccc attggcaaac tcgaataccg
      301 ccgtgagcgg ctgcggattc ttgtccacca ggatgtaaat cgcctgagtg ccgggtatct
      361 ggctggcgat ctccagcgga acaatggctc cgttctcggc aatttccggt gcttttaaca
      421 ggatccggtc gctgtcctcc atcgcggtga cgccaatgcc tttcagcgca tcggtaacaa
      481 ccttggcgtc gaatgcggcc ttgttccact ggccggccag cagttgcaac ggccgcagta
      541 tcgccgttgc catcgccacc gcggcggcgc gcaacacaga ccggcgcatc gttcggactc
      601 tcacgctctc catcgtttgg cgcttcactg cgtataaagg taatcgatga tcagttcgat
      661 ttcgtcgctg ctcaggatcc tgtgtttgcc gaacggcggc attaccgtgt tcggattggc
      721 ctgcatcgga tcccagaggc ggccgcgcag ctgcgcgcgg tccggaaacc tggagcggat
      781 cgccagcaga ggcgggccga tgttcgccag cgtatccgcc cccggatccc ccggaaacat
      841 gtggcaggcg gggcagttgc ctttggcgat ctcgaaggcg agtcgccttc ccgccgcgac
      901 attcggcgcc tcatctgcac gggtctgcga aattgaagga aaaaacgcgg tgccgatgag
      961 gacaacaaca atcaaccggc gatacagcgg gctgcgaccg tgctccaaag ctgagaccga
     1021 tccggcgcgc gccggttcct gcaagctcac ccgctgctgg tcccggcaac ccagtgcccg
     1081 gacttgccgc cgcgcttttc gcgcagcccg acgccatcga tgcgcatgcc ccggtccacg
     1141 gccttgcaca tgtcgtagac cgtgagcaat ccgacgctga ccgccgtgag cgcctccatt
     1201 tccacgccgg tgcgcccgac agtctcggca accgcgatgc attccaccga attgtcgtcg
     1261 cgatgcactt caaaatcgac gctcaccttg gtaagcgcga ccgggtggca cagcgggatc
     1321 aactccgagg tgcgcttcga accctggatt gccgcgatcc gcgccacacc gagcacatcg
     1381 cccttggccg catcgccttc gacgatcttc cgcagcgtat cggcacgcat gaaaatcctg
     1441 ccggacgcga tcgcgactct gcgggtttcc tgcttatccg ccacgtcgac catgtgcgcc
     1501 tgaccacggg aatcgaaatg cgtaagttcg cccaagctgg tatccgtctg tttcgcgtgc
     1561 ggccgcaacg cgaccgggga actggatgat aggtggcgct atcatagcac catgaaattt
     1621 cgctcagtga tcgctatggt cctgctggtc cctgcacttg gtctcggcga cgaattgccc
     1681 gacctgggcg atgtttcgca aggcgcgctg tcccccgccc aggagcgcaa gctcggagag
     1741 ggcatcatga gccagatccg cgccagcccg gtttacatcg acgatcccga cattagcgac
     1801 tatctgaacg ccgttggcta ccgattggtc ggcaacagtc cggatccggc cggcccgttc
     1861 gagttcttcg gaatcgccga aaactccgta aacgcatttg ccttgccggg cggattcgtc
     1921 ggtgtgcact cgggcttgtt gctcatcgca cagagcgagt ccgaactcgc cagcgtcctc
     1981 tcccatgaaa ttgcgcacgt cacccagcgc caccttgccc gcatggtcgc ggcgcaacag
     2041 cgtgccggaa tcgcgtcgat ggcggcgctg gcggtggcga tcctcgcggc gcgctcgaat
     2101 cccgaggttt ccgcggcggc gatcacggcg gcgcaagcgg gcacgatcca gtcggcgctc
     2161 aactttaccc gcgagcatga acgcgaggcc gaccgcatcg gtttacagat catggagaag
     2221 tccggcttcg acgtgcacgc gatgccggtg ttcttcgagc gcctgcagaa ggccacgcgt
     2281 gtatatgaaa ccggcgcacc cgcctatctg cgcacgcatc cgcttacttt cgagcggatt
     2341 gcggacgtgc agagccgcat cgagggcgtc ccgttcaggc aggtgcctga cagcctggaa
     2401 tttcacctcg tgcgtgcgcg gctgtcggcg cttgagggca atccacgcgt ggcggtcaca
     2461 aacttcgacg acatgctgtc cggccggaaa tttaccagcg aggcggcggc gcgctacggc
     2521 ctggtcgtgg cgctgttgcg cgcaggcgat tttgcgcgcg ccaggaaaga gctcgctccg
     2581 ttgcaaagcc tcaaggcgaa aagcccgatg atcgatgccc tggctgcgca aaccctcatc
     2641 ggcagcggcg agctcgagaa ggggctggcg acttatcgcg acgggctgcg cgccaacccc
     2701 agccgccgcg cactggttca cggctatgcc aacgccttgc tcgacgccac gcgcgcggcg
     2761 gaagcggtgg acttcctttc gggcaggctc gagactcgga cccccgacgc gcgcctttac
     2821 gagctgcaat cgcgcggcta taccgcattg ggaaagcact tgctgcagca ccgcgcgcag
     2881 gccgaggcgt acgtgctgcg cggaaacctt accgccgcaa tcgaacaact gcatctcgcg
     2941 cagcgcgccg gcgacggcga cttctatcag aaatccagcg tcgaggcgcg actcaaggaa
     3001 ctggtagcga tcgacactga agcccggcgc cgtccctgag tcacagtttt ttggcgtgcc
     3061 gtcgaaggcg ggtccggcga ccttatttag ccgcaatcgt ctgcggctcg ggcagtcgcc
     3121 ttgaatttac tcaaaaacct cgccttatag ctttgaattt cctatcatat cgacatccgc
     3181 cggcgtggcc gcgagggatt ccggcacagc gatcatggaa aagatcggca aatacgaaat
     3241 catcagggag ctcggcagag gtgcgaccag tgcggtttat ctggccaacg acgcatttgc
     3301 caaccgccag gtcgcgatca agctggtgcg ctccgatgcc ctcggcgaca aggaccacgg
     3361 caggcgcttt cataagctgt ttctcaccga ggcatcgctc gccgggaagc tttcccatcc
     3421 ccacatcgtt tccatcttcg atgcggttgt cgacgagcag ggcagctatc tggtgatgga
     3481 gtatgtcgag ggcggcaccc tcgagccttt gacccagccc gaatccctgc tgccggtcag
     3541 ccgcgtgatc gagatcattt tcaagtgctg caaggcgctc gattacgcgc atcgccacgg
     3601 catcattcat cgcgacatca agccggcgaa catactgctc acgggcgaat ccgacatcaa
     3661 gatatccgac ttcggcgccg cgctcacgat cgcagcggag accacgcagg tgtccggaat
     3721 cggctccccc gcgtacatgt cgccggagca actgcaggaa tttgtgctca atcaccagac
     3781 tgatatcttt tcgctgggtg tggtcatgca ccagatgctc accggggctc tgccgttcaa
     3841 ggcgaccaac aactacagca tggtctatca gatcattcac gtcgacgcgc cgccgccgag
     3901 catgctgcgc ccggagatcc cggccgccgt cgatgccatc gtacggcgcg cgctggagaa
     3961 ggagaccgcc aagcgattcc agacctggga ggaatttgcc caggcactcg ccgacagctt
     4021 caacaacctg cagaaaccgg aggaagtcgt cccggagacg gagaagttca atacgctgcg
     4081 ccggttggaa ttcttcagga ccttttccga cgtcgatctc tggcaggtgt tgcggatctc
     4141 caactgggcg cgctattccg ccaacagcct ggtgatcaag gaaggggatg tcggcacctc
     4201 gttctacgtc gtcgccgcgg gcgaggtcaa gatcaccaag cagggcaaac tgctcaacat
     4261 cctgcgcacg ggcgagtgtt tcggagaaat ggcctacctc ggcaagcgcc tgttccaacg
     4321 caccgcgagc gtcgcttcgg tcaacgacat caccatcgtc gaggtcggcg ccgattcgct
     4381 ggcacaggcg accgagagtt gcaagcacgc cttcaatgcg gctttcctcg agct
//
LOCUS       DASQOP010000352         2339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2401433, whole genome shotgun sequence.
ACCESSION   DASQOP010000352 DASQOP010000000
VERSION     DASQOP010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2401433"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VE030_13640"
     CDS             <1..393
                     /locus_tag="VE030_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZF24496.1"
                     /translation="IWGYAVGLDMTRRDLQQVGKDKGRPWTFGKDFDQSAPVGPITPA
                     SKSGHPKKGKLWLKVNGESRQNSDIDQMIWSVPEQIAFLTQYYTLEAGDLIMTGTPAG
                     VGAAKPGDELLAGIEGLGELRVKILPPR"
     gene            407..754
                     /locus_tag="VE030_13645"
     CDS             407..754
                     /locus_tag="VE030_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZF24497.1"
                     /translation="MSIKRHPSGKLFSAAVEHGGLVYVAGQVADDLTQGARGQTEQVL
                     KKIDAILAAAGTGKSKILSANVWVTDIRNRDEMNAAWTAWADPANLPARATVEAKLAD
                     PRMLVEIMVTCAK"
     gene            complement(774..2231)
                     /locus_tag="VE030_13650"
     CDS             complement(774..2231)
                     /locus_tag="VE030_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HZF24498.1"
                     /translation="MVRPFPVLNVLSVLIVVFALAMLLPLCVSLGYGDPARYAYEKAV
                     LITFVSGAILWVLTRSRQAELQTRDGFLLVALVWTVLPAFATLPLLMYMPELSFTDAY
                     FETVAALTTTGSTVLSGLDKLPPSINLWRCELQWLGGMGIIVLAVAILPLLGIGGSQI
                     MRTETPGPMKDTKLTPRITQTAKGLWLVYAAMTLACGLVFKAVGMGWFDAVLHSFSTL
                     SLGGFSSHDASFAYFDSPAIEAVAIFFMLVGGINFGTHFLAFRRLSLAPYRSDPEAGA
                     YVALLAISVLGVAYFLLANHVYPSFWTALRYSAFNVVSVATTAGFSSADFNQWPIFAP
                     VWMLFLCCFATCSGSTGGGIKMIRAELMVRQALRELMSIIHPRAYLPVKLSGQRVENN
                     IIFAVLAFMLIWGGSVIAMTMLLLASGLDIITAFSAVIVCICNTGPGLNLVGPATTYA
                     ALTDFQTWVCTVAMLIGRLELFTLFIVITPAFWRK"
BASE COUNT          529 a          699 c          754 g          357 t
ORIGIN      
        1 atctggggct acgcggtggg cctggacatg acccggcgtg atttgcagca ggtcggaaag
       61 gacaaaggcc ggccctggac cttcggcaag gatttcgacc agtccgctcc ggtcgggccg
      121 atcacccccg cgtccaagag cgggcatccg aaaaaaggca agctctggct caaagtgaac
      181 ggcgagtcgc gccagaattc ggacatcgac cagatgatct ggagcgtgcc cgaacagatc
      241 gcgtttctca cgcagtatta caccctggaa gcgggcgatc tcatcatgac gggaacgccg
      301 gccggggtgg gcgccgccaa gccgggggat gagcttctcg caggaatcga ggggctcggc
      361 gagctgcggg tgaagatact gccgccgcga tagacgggag gtcgacatga gcatcaaacg
      421 ccatccgtcg ggcaaactct tcagcgccgc agtcgagcac ggcggcctcg tctatgtggc
      481 aggccaggtc gccgacgatc tcacccaggg cgccaggggc cagaccgagc aggtgctgaa
      541 gaagatcgac gcgatcctcg ccgccgcggg caccggcaag tcgaagattc tttccgccaa
      601 cgtgtgggtc accgacatcc gcaaccgcga cgagatgaat gccgcctgga cggcatgggc
      661 ggatcccgcg aacctgccgg cgcgagctac agtcgaggcg aaactggcgg acccgcgcat
      721 gctggtcgag atcatggtga cctgcgccaa ataagccggg ttccgtaagg cggttatttt
      781 ctccagaacg ccggcgtgat gacgatgaac agcgtaaaca actccaagcg cccgatcagc
      841 atggcgacgg tacataccca ggtttgaaaa tcggtgagtg ccgcataggt cgtcgccggc
      901 cccaccaggt tcagccccgg cccggtgttg cagatgcaga cgatcaccgc cgaaaaagcc
      961 gtgatgatgt ccaggcccga ggccagaagg agcatggtca ttgcgatgac tgacccgccc
     1021 cagatcagca tgaacgccaa aactgcgaag atgatgttgt tctccactct ttggccggag
     1081 agcttgacgg gcagataggc gcgcggatgg atgatggaca tgagctctcg cagcgcctgg
     1141 cgcaccatca gttcggcacg aatcatcttg atgccgcctc cggtcgaacc ggaacaggtg
     1201 gcgaagcagc aaaggaacag catccatacc ggggcgaaga tcggccactg gttgaaatcg
     1261 gcgctggaaa aaccggctgt cgtcgcgacc gagacgacgt tgaacgcact gtagcgcagc
     1321 gccgtccaga aagacggata gacgtggttg gcaagcagga agtaggcgac tccgagcacc
     1381 gagatcgcga gcaaggccac gtaagcgccc gcctcggggt cgctcctgta aggcgccagg
     1441 ctcagcctgc ggaacgcgag gaagtgagtt ccgaaattga ttcccccgac gagcatgaag
     1501 aaaatcgcca cggcctcgat cgccggggaa tcgaagtaag cgaagctcgc gtcgtgggag
     1561 gagaatccgc ccaggctcag agtcgaaaat gaatgcaata cggcatcgaa ccaacccatg
     1621 ccgacggcct tgaacaccag accgcaggcg agcgtcatcg cggcgtaaac cagccacagg
     1681 cccttcgcag tttgggtgat gcgtggagtg agcttggtgt ccttcatcgg cccgggtgtc
     1741 tcggtcctca tgatctggct gccgccgatc ccgagcagcg gcagaatcgc gaccgcgagg
     1801 acgatgatcc ccattccgcc cagccactgc agctcgcagc gccataggtt gatcgaaggc
     1861 ggcagcttgt ccagccccga cagcacggtc gaaccggtgg tggtgagcgc cgccaccgtc
     1921 tcgaagtagg cgtcggtgaa gctgagctcg ggcatgtaca tcagcagggg cagggtggcg
     1981 aatgcgggca ggacggtcca aaccagcgcc accaaaagaa acccgtcgcg ggtctgcagc
     2041 tcggcctggc gagagcgggt aagcacccac aaaattgctc cgctcacaaa ggtgatcagc
     2101 acggctttct cataggcgta ccgtgccgga tcaccgtacc cgagcgatac gcacagaggc
     2161 aggagcatgg caagggcgaa gacgacgatc aagacgctca gcacgttgag tacgggaaac
     2221 ggccttacca tgggcgcgcg caatccgctt cagacgaagc cgacgctgac ctcgaaaagc
     2281 ttctccacct tgggcaggag tgtcttgtcg gtgacgaaga cgatcaagtg gtcctccgc
//
LOCUS       DASQOP010000353         6704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_184784, whole genome shotgun sequence.
ACCESSION   DASQOP010000353 DASQOP010000000
VERSION     DASQOP010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6704
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184784"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VE030_13655"
     CDS             complement(<1..409)
                     /locus_tag="VE030_13655"
                     /inference="COORDINATES: protein motif:HMM:NF017534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZF24499.1"
                     /translation="MAQDSSKSDFWESRYRDHVTPWDAGKVPASLQEYLPKMLKDARV
                     LIPGCGSAYEAGYLAENGFDVLAIDFSPAAVELAKKNLARLGEIARLADFFDFDYGSP
                     YDVIYERAFLCALPPTIWPQYAPRTAQLLRPGGK"
     gene            complement(633..998)
                     /locus_tag="VE030_13660"
     CDS             complement(633..998)
                     /locus_tag="VE030_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018915364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZF24500.1"
                     /translation="MKPERNYTISKNARRVSIADALDAVSKIREGIPSAFCFEHGTLL
                     VKMYRPSGQDPQKPHTRDEIYVIARGSGWFVNGTDRHPFQAGDVLFVPADVEHRFVEF
                     TEDFCTWVMFYGPEGGEAV"
     gene            complement(1042..1635)
                     /locus_tag="VE030_13665"
     CDS             complement(1042..1635)
                     /locus_tag="VE030_13665"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HZF24501.1"
                     /translation="MSEKTRCKWATGGNELYLDYHDQEWGVPQHDDRVLFEFLILEGA
                     QAGLSWSTILNKREGYRKAFDGFDPQKVARYDKTKINKLLQDSAIVRNRLKVEAAVLN
                     ARAFLEVQEEFGGFDTYIWRYTGGKPIQNSWKTMNQVPASTSESDAMSKDLKGRGFKF
                     VGSTIMYAHMQATGMVNDHTVNCFRWKQLQGRGTKNR"
     gene            complement(1643..2197)
                     /locus_tag="VE030_13670"
     CDS             complement(1643..2197)
                     /locus_tag="VE030_13670"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HZF24502.1"
                     /translation="MNRPSGLLTIGHSTLAADAFVAMLTAHSVELLADVRRFPASRRH
                     PQFGRDALAQMLASHGIAYLHFPELGGRRDPKPDSPNTAWREAGFRGYADYMETAEFS
                     HGISRLLDAAQARATAIMCAEKSWRSCHRGLIADHLKASGFDVIHVLDSAAAEPHPWT
                     AAARMIDGRLCYSAARIDQQQLPF"
     gene            complement(2194..2610)
                     /locus_tag="VE030_13675"
     CDS             complement(2194..2610)
                     /locus_tag="VE030_13675"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HZF24503.1"
                     /translation="MTAKAFTGGCLCGRIRYRTTGNPTNETHCHCEICRRASGAAFVS
                     WATFRSAAFVFTKGKPARFDSSDIAFRQFCSNCGTQLTFQFHKSPQTIDVTLASLDDP
                     GTITPVDHIWTKRQIAWIKLADGLPRYAESRSSEEV"
     gene            complement(2612..2962)
                     /locus_tag="VE030_13680"
     CDS             complement(2612..2962)
                     /locus_tag="VE030_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HZF24504.1"
                     /translation="MGYYAVKILVSAVLIVLISEIAKRSTLFGALIASLPITSLLAFI
                     WLYVDTGDSARVAALSTGVLWLVLPSLAFFVVLPTALRAGWAFWPGLLAAILATMACY
                     GIMVPILRKYGIEV"
     gene            complement(3038..3460)
                     /locus_tag="VE030_13685"
     CDS             complement(3038..3460)
                     /locus_tag="VE030_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24505.1"
                     /translation="MFTRINFLLGFGLLVAIGFYFAGNYLLVLPLSSVAPNWVKPIAG
                     VAVLVSLALTVQILSLVVSSMMGLASYTDTDGPGSLALTSQAAIVCGHAGLGYIAVAF
                     FSGERPVTERQFLVVAALYVAGIAIAILEWRQRGSDSR"
     gene            3889..4362
                     /locus_tag="VE030_13690"
     CDS             3889..4362
                     /locus_tag="VE030_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012213877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HZF24506.1"
                     /translation="MATKQSGLSARFEEFKDTWRTLSPYERIEQVIALVLTYLIAVII
                     LIATWDLAKEVFLLAWHGLLDPLDHRMFQVIFGQIMTLLIALEFKHSIIKVVAHRESI
                     IQVKTVLLIALLAISRKFIILEPNQYSAQTIVGLAAVVIALGVTYWLIRDRDAKA"
     gene            complement(4457..5533)
                     /locus_tag="VE030_13695"
     CDS             complement(4457..5533)
                     /locus_tag="VE030_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HZF24507.1"
                     /translation="MTSPRMDEHRRAATRAGLNYVTDGLAGITRRRAGRGWVYFAPDG
                     TRISDPAQRKRITALVIPPAWTDVWICPDPNGHIQATARDARGRKQYRYHPSYREARD
                     KSKFRRILEFSEVLPDIRDRVERDLRARDLSRRQILATVVRLLDRTLIRVGNDEYARE
                     NRSFGLTTLRGRHVQIKGATLRFSFRGKSGVQHDVAITDRRLARIVQQCQDLPGQELF
                     KFLDPAGKRQSVASDDVNAYLREITGRDITAKDFRTWAGTMLAARQLHTMRPVSSQRA
                     TEKNIVRAIDTVAKRLGNTRAVCRKYYVHPALLQAYQQGRTAPLPVPVGMPPHRKRRD
                     TPAPALRQEEVAVLQFLQEEAPGP"
     gene            complement(5608..6045)
                     /locus_tag="VE030_13700"
     CDS             complement(5608..6045)
                     /locus_tag="VE030_13700"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HZF24508.1"
                     /translation="MSTDAGTMQPVDSADILLVYDRECPVCDAYCRMVRIRESVGALR
                     LVNARDASGIMSEITAKGLDIDEGMVLKFGNTLYYGADAIHVLSLMSSRSGAFNRLTY
                     WIFRSKSRSTILYPLFRFFRNLLLKLLRKARINNLGLPRRERF"
     gene            6226..>6704
                     /locus_tag="VE030_13705"
     CDS             6226..>6704
                     /locus_tag="VE030_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HZF24509.1"
                     /translation="MEVRTAQGATAWAIALISFPYLALPLYWVFGKSKFHGYVTARRA
                     DLLEASPVAKGTYAALVERGLLVRTEYTRALPFERLAKLPFTTGNDAHLLIDGEATFD
                     SIFAGIEAARAYVLVQFYIIRDDDAGRELKARLLERARAGVRVHVLYDEIGSYELPRA
                     "
BASE COUNT         1303 a         1985 c         2086 g         1330 t
ORIGIN      
        1 gttttcctcc cggccgaaga agttgcgccg ttctaggcgc gtactgcggc catattgtcg
       61 gcggcagtgc gcacaggaac gcccgctcgt agatcacgtc gtaggggctg ccgtagtcga
      121 agtcaaagaa gtcggcgagc ctggcgattt cgcccaaacg ggcgagattt ttcttcgcca
      181 gctccacggc cgcagggctg aagtcgatcg ccagaacgtc gaaaccgttc tcggccagat
      241 aaccggcctc gtacgccgaa ccacaaccag gaatcagcac ccgcgcgtcc ttgagcatct
      301 tcggcagata ctcttgcaag gacgcaggca ccttgcccgc atcccacggc gtgacgtggt
      361 cgcggtagcg actctcccag aaatctgact tcgaactgtc ttgcgccatt taaatctcac
      421 cgcagaggcg caaaggcgca gaggaaacgc agagaaaacc aagcgaaacg tgtcaaaaag
      481 aaagaggatt ccaattcgcc accgccaatt cgatgaatca cggatgtcgc caatttttgt
      541 ttgtaatttt ttgcctttcc gtccaatttt gctttgcctt ccttggtgtt acctctgcgc
      601 ctttgcgcct ctgcggttaa ggggttgaga tctcagaccg cttcgccgcc ctcaggccca
      661 tagaacatca cccaggtgca gaaatcttcg gtgaattcga cgaagcggtg ctcgacatcc
      721 gccgggacga acagcacgtc gccggcctgg aagggatggc gatcggtgcc gtttacgaac
      781 cagcccgagc cgcgcgcgat cacgtagatc tcgtcgcggg tatgcggctt ctgcggatcc
      841 tgcccggagg ggcggtacat tttcactagc agggtgccgt gctcgaagca gaaggccgac
      901 ggaatgcctt cccggatctt cgacacagca tcaagggcgt ctgcaatgct caccctccgc
      961 gcattcttcg agatcgtgta gttcctctcc ggtttcatcg tcgttcgcct ttcacccttc
     1021 actcattcac ccttcacgcc ttcaccgatt tttcgtccct cgtccctgca actgcttcca
     1081 tcggaagcag ttgaccgtat ggtcgttcac catcccggtc gcttgcatgt gcgcatacat
     1141 gatggtgctg ccgacgaact tgaagccgcg gcccttgagg tccttgctca tcgcatcgga
     1201 ctcactggtg ctggcgggca cctggttcat tgtcttccag gaattctgta tgggcttgcc
     1261 gccggtgtat cgccagatgt aggtgtcgaa gccgccgaac tcctcctgca cttcaaggaa
     1321 cgcccgggca ttgagcaccg ccgcctcgac cttaagccgg ttacgcacga tcgcagagtc
     1381 ctgcagaagc ttgttgattt tcgtcttgtc gtagcgtgcc accttctgcg gatcgaatcc
     1441 gtcgaacgcc ttgcgatagc cttcgcgctt gttgagtatg gtggaccagg acaggccggc
     1501 ctgcgcgcct tccaggatca ggaactcgaa cagcacccgg tcgtcgtgct gcggtacgcc
     1561 ccattcctga tcgtgatagt cgaggtagag ctcgttaccg cctgttgccc atttgcaacg
     1621 tgtcttttcg ctcattgttc tctcagaaag gcagttgttg ttggtcgatt cttgcggcgg
     1681 agtaacagag ccggccgtcg atcatccgtg cagcggcagt ccagggatgg ggctcggcag
     1741 cggctgagtc gaggacgtgg atgacatcga atccagacgc cttgagatgg tcggcaatca
     1801 gcccgcggtg gcaactgcgc caggactttt cagcgcacat gatcgccgtg gctcttgcct
     1861 gtgcagcgtc caacaggcgt gaaatgccgt gactgaattc cgcggtttcc atgtagtccg
     1921 cgtagccgcg gaatcctgcc tcgcgccacg cggtgttggg tgaatccggc ttcggatcgc
     1981 gccgtccgcc cagttccgga aaatgcaggt acgcgatacc gtggcttgcc agcatttgcg
     2041 ccagcgcgtc gcgaccaaac tgcggatggc ggcgggaagc agggaagcgg cgcacgtcgg
     2101 caagcaactc gacgctgtgc gccgtgagca ttgccacaaa cgcatcggcc gcaagcgtcg
     2161 agtggccgat ggtcagtagt ccggacgggc ggttcacact tcctctgaag atctgctttc
     2221 tgcgtaccgc ggcagtccgt ccgccagctt gatccaggca atctgcctct tggtccagat
     2281 gtggtccacc ggcgtgatcg ttccgggatc atccaggctg gcgagtgtga cgtcgatcgt
     2341 ctgtggcgat ttgtggaact ggaaggtcag ttgcgtgccg cagttcgagc agaactggcg
     2401 aaatgcgatg tcggaagaat cgaaacgtgc cggtttgccc ttggtgaaga caaatgcggc
     2461 ggaccggaaa gtcgcccagg acacgaaagc cgcgccgctt gctcgccggc agatttcgca
     2521 gtggcagtgg gtttcgttgg ttggattgcc agtggtccga tagcggatgc gtccgcacag
     2581 gcaaccgccg gtaaaggcct tcgcagtcac gtcagacctc gatgccgtac tttctcagga
     2641 tcggaaccat gatgccgtag caggccatgg tagcgagtat ggcggcgagg aggcccggcc
     2701 agaacgccca ccctgcccgc aacgcggtag gcagcacaac gaaaaatgca agcgagggca
     2761 gcaccagcca gagtactccg gtggacagcg cggcaaccct ggcactgtcc ccggtgtcga
     2821 cgtagagcca gatgaacgcc agcagcgaag taatcggaag cgacgcgatc aatgcgccga
     2881 acagggtgct gcgtttggcg atctcggata tcagcacgat cagtaccgcg gacaccagga
     2941 tcttgactgc gtagtagccc atctccttcc tttttcagtt tgttccggca gtctcgacca
     3001 ggaacaaccg cactggctcg cctctgtcat ttcgcggtta tcttgaatcc gagccgcgtt
     3061 ggcgccattc caggatcgcg atcgcaatac ctgccacata cagcgcggcc acaaccagaa
     3121 attgccgttc ggtgaccggt cgttctccgg aaaagaaggc caccgcgatg taacccaggc
     3181 cggcgtggcc gcagacgatc gctgcctggc tggtgagcgc gagcgagccg gggccgtcgg
     3241 tatccgtgta actggccaag cccatcatgc tactcaccac caaggacagg atctgcaccg
     3301 tcagcgcgag actgaccagg actgccactc cggcaatcgg cttgacccaa ttcggcgcga
     3361 cgctcgacag cggcaacacc aacaggtaat tgccggcgaa atagaagccg atggcgacca
     3421 gcaggccgaa gccgaggaga aaattgatcc gggtaaacat actcaggtgt tgaacacgat
     3481 agcagggatg gggcgttctt ggacgagaat ttcggcaatg tgatgtgcca gatgggggcg
     3541 caaggatgaa ggcgtaagga tgaaggagca aggatgaagg ccgaaggatg aaggatgagg
     3601 gaacaaagat gaaggatgag ggagcaagga tataaggcgt gtagcgaagg agacgccagc
     3661 cggccaaagg ccggcattaa aagcgttgac gcaaaggacg caaaggagcg caaagttcgc
     3721 aaaggaatac aaaaaaagta cgaagatagg ttgctgtttg cctctgcgtt ccttcttcat
     3781 ccttccgcct tcatccttca tccttcatcc ttgctctcac acccactgga cttttcatat
     3841 atgacggcag atactgcggc gaccgattgt catggtgcga ggtgggtgat ggccacgaag
     3901 caatccggat tgagcgcgcg tttcgaagaa ttcaaggaca catggcgcac gctgagccca
     3961 tatgagcgca tcgagcaggt gatcgcgctg gtgctcacct acctcatcgc ggtgatcatc
     4021 ctgatcgcga cctgggacct cgccaaggag gtattcctgt tggcgtggca cggacttctc
     4081 gatccgctcg atcacaggat gttccaggtg atcttcggcc agatcatgac cctgttgatc
     4141 gcgctcgagt tcaagcactc gatcataaaa gtggtggcgc atcgcgagag catcatccag
     4201 gtcaagacgg tgctgctgat cgcgctgctg gcgatctcgc gcaagttcat catcctcgaa
     4261 cccaaccaat actcggcgca aaccatagtc ggccttgccg ccgtggtgat cgcgttgggc
     4321 gtcacctatt ggctgatccg cgaccgcgac gcgaaggcct gagtcagcga cgggcccgcg
     4381 gttgcgagat gaggacggcg gttcagtacc aggatcgagc agggcgggaa tcggccgatg
     4441 ggccgctgaa gcgggcctaa gggccgggag cttcttcctg gaggaactgc agcaccgcga
     4501 cttcttcctg gcgcagcgcc ggcgcggggg tgtcgcgacg cttgcggtgt ggcggcattc
     4561 ccactggcac gggcagcggc gcggtgcggc cttgttgata agcttgcaac agggctggat
     4621 ggacgtaata tttccggcac acggcgcggg tattgccgag ccgcttagcc acggtgtcga
     4681 tcgcacgcac gatgttcttt tcggtcgcgc gctggctcga taccggcctc atggtgtgca
     4741 actggcgggc cgccagcatc gtgcccgccc aagttcggaa atccttggca gtgatatcgc
     4801 ggccggtgat ctcccgcaga taggcgttga cgtcgtcgga cgcgacggac tggcgtttcc
     4861 cggcgggatc caggaacttg aacaattcct gtccgggcag gtcctggcat tgctggacga
     4921 tgcgggcgag acggcggtcg gtgatcgcca cgtcatgctg gaccccgctc ttgccgcgga
     4981 agctgaaccg cagcgtcgca cccttgatct gaacgtggcg cccgcgcagc gtggtgagcc
     5041 cgaacgaacg gttttcgcgc gcgtactcat cgttgccaac ccggatcagc gtgcggtcga
     5101 gaagccgcac cacggtggcg agaatctggc gccgcgacag gtcgcgggcg cgcagatcgc
     5161 gctccacgcg atcgcggata tccgggagta cttcgctgaa ttccaggata cggcggaatt
     5221 tggacttgtc gcgtgcttcc cggtacgagg ggtgatagcg gtactgcttg cggccgcgcg
     5281 cgtcgcgcgc ggtggcctgg atgtggccgt tgggatcggg gcagatccag acgtcggtcc
     5341 acgccggcgg aatcaccagg gcagtgatgc gcttgcgctg cgccgggtcg ctgatgcgtg
     5401 ttccgtccgg agcaaagtat acccatcccc tgccggcacg ccggcgggtg attcccgcca
     5461 ggccgtccgt gacatagttc agtccggcgc gggtggctgc cctgcggtgc tcgtccatgc
     5521 gcggcgaagt caccgaatcc tccttccctg gaattgcggc ggacacagag tatccggatg
     5581 ctcccgcgga aggcaagcgc tgaacggcta aaacctttca cggcgaggga gccccaggtt
     5641 gttgatccgg gccttgcgca ggagcttgag cagcaggttg cggaagaatc gaaacaacgg
     5701 gtaaaggatg gtggaccggc tcttcgagcg gaatatccag taggtgagcc gattgaacgc
     5761 gcccgagcgg ctgctcatca acgacaggac gtggatggcg tcggcgccgt agtacagggt
     5821 attgccgaac ttgagcacca tcccctcgtc gatatcgagg ccttttgcgg tgatctcgct
     5881 catgatcccg ctggcgtcgc gcgcgttgac caggcgcagt gcgccaaccg actcgcggat
     5941 gcgaaccatg cggcagtagg catcgcagac cgggcattcc cggtcgtaca ccaggagaat
     6001 atcggctgag tccacgggtt gcatggtgcc tgcgtcggtg ctcatggtgg cggctcgccc
     6061 ggattgacga tcacaatgta tattccgatt gcgacctctt tcgtacctcc cggcggggga
     6121 cgccgacccg tcgcaaacca tcctacattc atcgcttccc cgatgctcgg cttacttatc
     6181 gccctcacac aactgcttgg cctgataacc gccgcgaggg cggtcatgga ggttcgcact
     6241 gcacaagggg cgaccgcgtg ggcaatcgcg ctgatctctt tcccgtacct ggcgctgccg
     6301 ttgtattggg tgttcggcaa aagcaagttc cacggttacg tcaccgcgcg cagggccgat
     6361 ctcctggagg cgagcccggt tgccaaggga acctatgccg ccctggtcga acgcggattg
     6421 ctcgtgcgga cggagtacac gagggcgctg ccgttcgaac ggctggcgaa gctgccgttt
     6481 accaccggaa acgacgcgca cttgctcatc gacggggagg ccaccttcga ttctatcttt
     6541 gcgggaatcg aagcggcacg cgcatacgtg ctggtgcagt tctacattat ccgcgacgac
     6601 gatgcggggc gcgagctcaa ggcgcgcctg cttgagcgcg cccgcgccgg cgttcgcgtc
     6661 catgtgctgt acgacgagat cggtagctac gaactgccgc gggc
//
LOCUS       DASQOP010000354         3772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1794825, whole genome shotgun sequence.
ACCESSION   DASQOP010000354 DASQOP010000000
VERSION     DASQOP010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1794825"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VE030_13710"
     CDS             complement(<1..456)
                     /locus_tag="VE030_13710"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: protein motif:HMM:NF006599.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HZF24510.1"
                     /translation="MLGIIGGTGLTQLSNLEITRRQVVRTPYGEPSGALTFGRLGGRE
                     VVFLARHGHGHTIPPHAVNYRANIWALHSEGVSRVVSVASVGGIRADLKPGTLALPSQ
                     IVDYTYGRKHTFFDGPDQPVTHVDFTHPYCNKLRGQLLDAAGRAKQAIVD"
     gene            complement(468..1013)
                     /locus_tag="VE030_13715"
     CDS             complement(468..1013)
                     /locus_tag="VE030_13715"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HZF24511.1"
                     /translation="MLDKNQAWQVLREAELVCSEEQVDRAVERVAAEITARLADANPL
                     VLSVMGGAVVFTGRLLPLLHFPLELDYLHVTRYDGKTRGSEVRWKAGPRTDIRSRTLL
                     VVDDILDEGDTLAAVRDRLLAEGAAAFFSAVFADKDIGRPKPIRADFVGVRVPDRYVF
                     GFGMDINEYWRNLPAIYAVKA"
     gene            complement(1003..1896)
                     /gene="galU"
                     /locus_tag="VE030_13720"
     CDS             complement(1003..1896)
                     /gene="galU"
                     /locus_tag="VE030_13720"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015603990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HZF24512.1"
                     /translation="MITKAVFPVAGLGTRFLPATKASSKEMLPVVDKPLIQYAVEEAV
                     AAGITQMVFITGRNKRAIEDHFDKAYELETELQARGKLKMLEQVQDIVPKHISCIYVR
                     QTEALGLGHAILCARPVVGNAPFAVILADDLIDGDPPVMKQMVDIYARERCSLLAVQT
                     VPREDTSSYGIVKTEPGERGMHRITGIVEKPRPENAPSNLAVVGRYVLTPEIFGHLEK
                     VKPGASGEVQLTDGIAALLDEQRALAYEFQGARHDCGSKLGYLQANVLYALRHPEIGN
                     DFSVFLDSLPLKSKKPRSNAG"
     gene            complement(1920..>3772)
                     /gene="ligA"
                     /locus_tag="VE030_13725"
     CDS             complement(1920..>3772)
                     /gene="ligA"
                     /locus_tag="VE030_13725"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018444191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HZF24513.1"
                     /translation="SPTRRIGAMPAEAFAQVTHPTPMLSLNNAFGEAEVEAFDRRVRE
                     GLGVAGPIEYAAEPKFDGLAVSLTYDKGIFAMGATRGDGYTGEDVTANLRTVRSIPLR
                     LEGRNLPDLLEVRGEVLMLKRDFQALNREQQKKGEKEFVNPRNAAAGALRQLDSRITA
                     GRRLAFFAYGLGRVSGGKFPADRHSHQLDCLESLHFPVARERSVVKGVEGLLDYYREI
                     GSRRDSLPFDIDGVVYKVDDLEFQALLGFVSRAPRFAIAHKYPAQEEITQVLGIDVQV
                     GRTGALTPVARLAPVFVGGVTVTNATLHNEDEVKRKDVRIGDYVIVRRAGDVIPEVVS
                     VVKERRPPGVRSFRMPSKCPVCGSNVERLEDEAVARCTAGLYCPAQRKQALLHFAGRR
                     AMDIEGLGEKLVDQLVDQGLVKTPADLYRLDAKDLASLERMAEKSAANVLAAIEKSKR
                     TTLARFIYALGVRNVGEATARDLARHFGKLEPLMQAGEDELQQVPDVGPVVARSIARF
                     FGEPHNREVIAQMRKLGVTWPESTVTRPPRQPLAGKTFVLTGTLPHLSRDQAKERIEA
                     KGGKVSGGVSRKTDYVVAGAEPGSKYDKALELGIAVLDERGLLELLGRDN"
BASE COUNT          673 a         1229 c         1159 g          711 t
ORIGIN      
        1 atccacgatt gcctgttttg cccgccctgc cgcatcgagc aactggccac gcagcttgtt
       61 gcagtaagga tgggtgaaat ccacatgcgt aaccggctga tccggaccgt cgaagaaagt
      121 gtgcttgcgc ccgtaggtgt aatcgacaat ctggctgggg agcgccagtg tgcccggctt
      181 caggtcggcg cggatgccgc cgaccgatgc gaccgagacc acccgcgaca ccccctccga
      241 atgcaacgcc cagatattgg cccggtagtt caccgcatgc gggggaatgg tgtgcccgtg
      301 gccgtggcga gccaggaaca cgacttcgcg gccgccgagt ctgccgaaag tgagcgcgcc
      361 ggaaggttcg ccgtaagggg tgcggaccac ctggcgccgc gtgatctcaa gattggagag
      421 ttgggtcaag ccggtgccgc cgattatgcc gagcatggtt tcaggattca ggctttcact
      481 gcgtagattg cggggagatt gcgccagtat tcgttgatgt ccatgccaaa tccgaaaaca
      541 taacggtcgg gcacgcgcac gccgacaaaa tcggcccgga tcggcttggg gcggccgata
      601 tccttatcgg cgaacaccgc gctgaaaaag gcagccgcgc cctcggccag caatctatcc
      661 cgcaccgcgg ccagcgtgtc accttcgtcg aggatgtcgt ccaccaccag cagcgtgcgg
      721 ctgcgaatat cggtgcgcgg cccggctttc catcgcactt ccgagccgcg ggttttgccg
      781 tcgtagcgcg tgacatgcag ataatcaagc tccagcggga aatgcagcaa gggcagcagt
      841 cggccggtga acaccacggc tccgcccatg accgacagga ccagcgggtt ggcatcggca
      901 agcctggcgg tgatctctgc cgcgacgcgt tcgacggcac ggtccacttg ctcctcggaa
      961 cacaccagtt cagcctcgcg cagcacctgc caagcctggt tcttatccag cattcgagcg
     1021 aggcttcttc gatttgaggg gcagcgaatc gagaaaaacg gaaaaatcgt tgccgatctc
     1081 gggatggcgc agcgcgtaca gcacgttcgc ctgaaggtag ccgagcttgg atccgcagtc
     1141 gtggcgcgcg ccctggaact cgtatgccag agcgcgttgc tcgtcgagga gcgcggcaat
     1201 gccgtcggtc agctggacct cgccgcttgc tcccggtttg accttctcga ggtgaccgaa
     1261 gatctccggc gtcagtacgt agcgtccgac caccgccaga ttggaaggcg cattctccgg
     1321 ccgcggcttc tcgacgatgc cggtgatgcg gtgcatgccg cgttcccccg gctcggtctt
     1381 gacgattcca taactcgagg tatcctcgcg cggcacggtc tgcaccgcca gcaacgagca
     1441 gcgctcccgc gcatagatgt ccaccatctg cttcatcacc ggcggatcgc cgtcgatcag
     1501 atcatcggcg aggatcaccg cgaacggtgc attgccgacc accggccgtg cgcacaggat
     1561 cgcatgaccg agaccgagag cctcggtctg gcgcacatag atgcagctga tgtgcttggg
     1621 cacgatgtcc tgcacctgct cgagcatctt cagcttgccg cgcgcctgca gttcggtctc
     1681 cagctcgtag gccttgtcga aatggtcctc gatggcgcgc ttgttgcgcc cggtgatgaa
     1741 caccatctgg gtgattcccg cggcgaccgc ctcttccacc gcgtactgga tcagcggctt
     1801 gtcgaccacc ggcagcattt ccttcgagct cgccttggtc gcaggaagaa agcgcgtacc
     1861 caatccggca accggaaaga cagctttggt gatcatgttc aatgcgggct ccgcgttgtt
     1921 cagttgtccc ggccgagcaa ttctagcaac ccccgctcgt cgagcacagc gataccgagt
     1981 tccagcgcct tgtcgtactt gctccccggt tccgcgccgg ctacgacgta atcggttttc
     2041 ctggatacgc cgccggagac cttgccgccc tttgcttcga tgcgctcttt cgcctggtcg
     2101 cgcgagagat ggggcaaggt accggtcaac acgaaggttt ttccggcgag cggttgacgc
     2161 ggcggcctgg ttacagtcga ttccggccag gtcacgccaa gtttgcgcat ctgcgcgatc
     2221 acttcgcggt tatgaggttc accgaagaac cgggcgatgc tgcgcgccac caccgggccg
     2281 acatcgggca cctgctgcag ctcatcttcg cctgcctgca tcagcggctc cagtttgccg
     2341 aaatggcgcg ccaggtcccg ggcggtcgct tcgccgacgt tgcggacgcc gagggcatag
     2401 atgaagcgcg ccagcgtcgt acgcttgctc ttctcgatcg ccgcaagaac gttggcggcg
     2461 gatttttccg ccatgcgctc caggcttgcc aagtccttcg catccagccg atagagatcg
     2521 gcgggcgttt tcaccagtcc ttggtccacc agttgatcga ccagtttctc gcccagtcct
     2581 tcgatatcca ttgcccgacg cccggcaaaa tgcagcagcg cctgcttgcg ttgcgccgga
     2641 caatacagtc cggcggtgca gcgggccacc gcctcgtcct ccagccgctc gacgttcgac
     2701 ccgcacaccg ggcacttgga gggcatacgg aacgaccgta caccgggcgg gcggcgctcc
     2761 ttcaccacac tgacaacttc cgggatgacg tcgcccgccc ggcgcacgat gacgtagtcg
     2821 ccgatgcgca cgtccttgcg tttgacctcg tcttcgttgt gcagcgtagc gttggtcacg
     2881 gtgacgcccc ccacgaagac gggcgcaagc cttgcaaccg gcgtcagtgc gccggtacgg
     2941 ccgacctgca cgtcgatacc gaggacctgc gtgatctctt cctgcgccgg atacttgtgc
     3001 gcgatcgcga agcgcggggc gcgtgaaacg aagcccagca gggcctgaaa ttcgagatcg
     3061 tccaccttgt aaaccacgcc gtcgatgtca aaaggcagcg agtcgcgacg actcccgatt
     3121 tcgcggtaat aatccaggag cccctccacg cccttgacga cgctgcgctc cctggcgacc
     3181 ggaaaatgca gcgattcgag gcagtcgagt tgatggctgt ggcgatccgc cggaaatttt
     3241 ccgccgctca cgcggcccag accgtaggcg aaaaaggcca ggcgccggcc ggcagtaatg
     3301 cgcgagtcga gctgtcgcag cgccccggcg gccgcgttgc gcgggttgac aaactccttt
     3361 tcgccctttt tttgctgctc gcgattcaag gcctgaaagt cgcgcttgag catcaacact
     3421 tcgccgcgta cttcgagaag atccggcagg ttgcggcctt cgagccgcaa cgggatagag
     3481 cgcaccgtgc gcagattggc ggtgacgtct tcgccggtat agccatcccc ccgggtcgcg
     3541 cccatggcga aaatgccctt gtcgtacgtg agactgacgg caagaccgtc gaacttgggc
     3601 tctgctgcgt actcaatcgg ccccgcaacc ccgaggcctt cgcgcacgcg ccggtcgaaa
     3661 gcctcgactt cggcttcgcc gaacgcatta ttgagcgaca gcatgggcgt cgggtgcgtg
     3721 acctgcgcga atgcctcggc cggcattgcg ccaatgcgcc gggtaggaga gt
//
LOCUS       DASQOP010000355        14529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_784043, whole genome shotgun sequence.
ACCESSION   DASQOP010000355 DASQOP010000000
VERSION     DASQOP010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14529
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784043"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1715)
                     /locus_tag="VE030_13730"
     CDS             complement(<1..1715)
                     /locus_tag="VE030_13730"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HZF24514.1"
                     /translation="MPEEQSFVWPTPPYYQYASAQDAKLPRSCLLVGNDGRQMQGRLI
                     RFLPEQGTLDFVATGLTSIETLQFARIKKLQLLRPVQLLPRELEKMQNAGLVPPTGNQ
                     IVKVSFKDGEKLAGETRGFLIEMTGLYLYLTVEDGKIQRTFIPTHAYADYSIGQSLGE
                     MLTEQRKDTMDFVEVGLQLQQHLRTQRIGDYLTASQIISREFLEQVLKDRDQTLPESQ
                     LGQVLVREEVISKEELDKAVSLQQRDRKLPLGEILIEMGVIDRASVNRLLVRKLGIPF
                     VDPTRFDIEQRALALVERRLAEKHRVIPLFRTKDEIVVAMENPTDPVPVQELRFSTRL
                     RVVPVMANQENILSAIKTYYGQYEAARNVKDLAASAPAAAKAAEHDDPGVGVSDNALV
                     RIVNQIIQDASERKASDIHVETYRGKEDTKVKLRIDGELEDYLDVPAHFRNAMISRIK
                     IMSNLDISERRKPQDGKIVFNRFGTANVELRVAIMPTTDGLEDVVMRVLAAAKPIALD
                     KMGLREPVLAEIKRIAAMPYGLMLICGPTGSGKTTTLHSVLGHINNGRKKIWTAEDPI
                     EITQKG"
     gene            1886..2719
                     /locus_tag="VE030_13735"
     CDS             1886..2719
                     /locus_tag="VE030_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZF24515.1"
                     /translation="MKTVALSSGERVPALGQGTWCMGENRAARAEEIATLRLGLDLGI
                     TLIDTAEMYGEGKAEELIGEAIAGRRDQAFLVSKTYPHNATRKGAVAACERSLRRLRT
                     DRIDLYLLHWRGSVPLAETMEAFMALQKDGKIRHYGVSNLDLDDMQELRSVPGGKGVA
                     TDQLLYNLARRGIEWDLLPWLRKRHIPVMAYSPIERASLARNSKLADFARRHGMTPAQ
                     AALAWLLAKDDIIAIPKTAHRERLKENAGALELTLSSEQLAKLDNLFPPPKGPRPLEM
                     L"
     gene            complement(2816..4948)
                     /locus_tag="VE030_13740"
     CDS             complement(2816..4948)
                     /locus_tag="VE030_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HZF24516.1"
                     /translation="MAMNRREFIKVSALAGGGFVIGCALPATQALAAEGKTFAPNQWI
                     RITPDNIVTVVVDKSEMGQGVYTGLPMLIAEELDVDWSKIRIEPAPAAKEYAHPWFGV
                     QGTGGSTSIRAMWQPLRQAGATARAMLVAAAAETWKVDPAKLSTEYGAVIGPGGKRAT
                     YGQLAAKAAGIAPPKEVPLKDPAAFKIIGKPLKRLDTPAKTNGSAKFGMDAKLPGLLT
                     AVVVRSPVVGGKLVRFNADKAKAIKGVKMVLPVTSPTSEGVAVLAQDYWAAKLGRDVL
                     EIEWDAGPNATLSTASLREQMSKFAESGEGALLARKEGDVAAATVAKTVEALYEVPYL
                     VHAPMEPMNCTAWVKPDGVELWVGSQAQGPNQMTAAMIAGCKPEQVKIHTLLLGGGFG
                     RRFAPDFLVEAVSLSKAAKAPVKVVYSREDDTKAYYYRPMAIAKLKGGLDAEGKAVSF
                     EATTVCDSLAEGTGFEAMIIKDRIDGTSVEGLADIPYSIANVKVDWVKYQPGVRTWFL
                     RSVGSTQNVFFSESFIDEMAHAAGKDPFEFRRALLEKHPRHKAVLELAADKAGWGSPL
                     PQGRARGIAVAESFGSFVAQVAEVSLEDDRPRVHRVVIAADVGTVVNPNQVVAQMEGA
                     MVYGLSAALYGEITLKDGKVEQSNFNDYPVVRMNEMPVVEVHLVKSAENPGGVGEPGT
                     PPIAPAVANAIFALTGKRHRKLPLAKPA"
     gene            complement(4961..5419)
                     /locus_tag="VE030_13745"
     CDS             complement(4961..5419)
                     /locus_tag="VE030_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZF24517.1"
                     /translation="MLALNVNGRTRKVDVDESTPLLWVLRDHLDMTGTKFGCGMALCG
                     ACTVHLDGQPIRSCVTPASMAVGKKITTIEAIGRTPAGRKIQAAWVALDVPQCGYCQS
                     GQIMSAAALLKSNPKPGDADIDSAMSGNICRCGTYGRIRAAIKQAAGVSV"
     gene            5873..7120
                     /locus_tag="VE030_13750"
     CDS             5873..7120
                     /locus_tag="VE030_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24518.1"
                     /translation="MKRGTGLNNFAGAEGGTPSLSPKIRDALAKLDDALFAIWDLGDN
                     VLLAWTHEDGELRFLAVRHYTIAESVHSGAAHPNQKKDAIGFVNEILAGSKHLAPDRF
                     REVCREFGADPQTLRVDFRVGTDVDVERINELVRRYSVSLVRGRAVVLLDAVEFSLQP
                     PLDQMAMLNSLSFSVNSAYRQLLSKEIDVNFARTTTGDGFYVWNRATNPGANVELYKL
                     MMLILADNAVAQRKARSEWVPKLRAAFHVGEHYEFHQVEGLNPTTFSYIVGQVTVDLA
                     RMIEKSLPGQILLGDFEVREEIRPGRFIRYDTVDFVERTAATLDHLNGLVISGGRIEN
                     IRCYLTGQSLGSGRYMVNRYHIRDKHDTSRVVYNAKINIHRENTEPIFLGIQRDDLWA
                     FATAKVETLNRDAEQTGSYFIRP"
     gene            complement(7204..8124)
                     /locus_tag="VE030_13755"
     CDS             complement(7204..8124)
                     /locus_tag="VE030_13755"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZF24519.1"
                     /translation="MTVKEVVRTRAEPQTQYASRARYHTSENPFTFGYPPVPARQFLA
                     ERDRAFDPALPTGAIALDAAGNLGTPYPATSPTLLCRYLKIRASEHLRAAFAASGAIY
                     YVMAGCGESRNAADVIAWGAGDVFCFPGGSETTHRAAGADCLLFVATDEPLLAFERLR
                     PPAPRESVVDTVHWPAHEIERRFEAVWQRPVTENTTGHSVMFSTAALAPGYATIPSIN
                     VAINTLAPGADQRPHRHNGVAVTLAIQGEDIYSMIGGQRVNWSTGAAQITPATLLHSH
                     HNRGVKRMRSLVIQDEGLHHYTRTPGFSFD"
     gene            complement(8553..10046)
                     /locus_tag="VE030_13760"
     CDS             complement(8553..10046)
                     /locus_tag="VE030_13760"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZF24520.1"
                     /translation="MSDDILSLTAVELTAHYRSKKLSPVEATRAALDRIARLNPLYNA
                     FVMVDETRALKDAQASEKRWQRGMPAGSVDGVPTTVKDLIVTEGWPTLRGSRTIDPKQ
                     PWTEDGPPVARMKEQGAVFLGKTTTPEFGWKGVTDSPLTGITVNPWNTRLTPGGSSGG
                     AAVAAAFGMGSLHIGTDGGGSIRIPAGFCGLFGFKPTFGIVPVHPHSPAATLWHQGPI
                     SRNVADAALMLTVIARPDPRDWYQAPARNIDYRQELDSGVKGLRIGYSPTLGYAHVDP
                     EVAMLVEEGARVFESLGARVEELDLELEDPIAIMQPLWAVALALAIAPMTTAQRALVD
                     PPLLELAEPGFGLSALEYRRLERAREAFGRRMCMLHQDYDLLITPQLAVAAFEAGYEV
                     PPGSGRTRWWEWSPFTYPFNLSQQPAAAVPCGFTREGLPVAMQLVGDRFADARVLQAA
                     RAYEAGHPFVMPEPDPDPAALPQTTDSSPREGRKGRGGNARERTHWS"
     gene            complement(10463..12925)
                     /locus_tag="VE030_13765"
     CDS             complement(10463..12925)
                     /locus_tag="VE030_13765"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HZF24521.1"
                     /translation="MVKPITAETQTQAMTRREFLVRSTVIAGSGFFSIGMPALLGGKN
                     AEAAMATPAFTPAIWFTITPDGKTTMHIVKAEMGQHIGTGLAQVIAEELEVRWEDVRL
                     DAPLESVENFGVYGLAYTVNSGSVTTEFDRIARAGAAGRIALVEAGAKVLNAKLADCH
                     AEAGRVVDKASGRSVGYGEILQKVKIDRKFAYPDDLKGITLKDPSQYKIIGKSFAALD
                     IPAKTNGKAKYGIDVFLPNMAYGALVVPRSRYASKVLSIDDSEAKKIPGFIKAVKIDN
                     AMGKCTGWVVAVADKFPTAMKAAKALKVKWDAGPYANLSSAEMLEQYKQLAKDEKQGA
                     NWVLEGDVDKGFGDAEKVLEVEYTTDMVCHATMEPLNATVQLTNGEWHVHVGTQSTSF
                     ARMTLTGYLSNVLKKKPEDIKVFVHQYLVGGGFGGKQDYDDILAAAYCVKETGRPVKL
                     IHTRESNFATSFARTPTYHKVKAGIKNGELTAMNQDIVCGWMGARFGVGKAGGTDWLQ
                     LDSWDAKKQDIDQWSIGGSDHWYNVKNHRVRAWNHEPTTWAVQASALRTVSNSYNIFV
                     VESFMDEVARAAGRDPLEFRLAMLNGKGGNRGIPNSGYPAGTGSDYYMDQLWISLPWA
                     KEGSWPVYESGTVGGALRLANCLRVAAGKSGYGTKKLPPNTGMGIAVTAAEERQSPTW
                     VAGVAEVTVDPATGRYSINRITIAMDPGIAVNPGNIEAQIRGSALWGASQIMSEKLTL
                     KNGSFEQSNFHEYQPIRLAQVPEIDVTIIQSKHHPAGVGEPASTVVAPAVANAIANAV
                     GARVRHMPISPEAVLAAMKQKA"
     gene            complement(12918..13394)
                     /locus_tag="VE030_13770"
     CDS             complement(12918..13394)
                     /locus_tag="VE030_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZF24522.1"
                     /translation="MPQLSINGQSYFTDSPPDTPLLWAIREDLRLTGTKYGCGLGVCG
                     SCTVHINGQPVRSCMVKLGDVESNAVITTIEGLDPADAHPVQKAWIEAQVPQCGYCQS
                     GQIMQAAALLAVNPNPTSEEIVSHMNGNLCRCATYSRIVGAVHRAADLMKEGSNHG"
     gene            complement(13808..14362)
                     /gene="msrA"
                     /locus_tag="VE030_13775"
     CDS             complement(13808..14362)
                     /gene="msrA"
                     /locus_tag="VE030_13775"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HZF24523.1"
                     /translation="MEQAREGQEIATLAGGCFWCLEAVYDRLQGVESVESGYMGGQRP
                     DPTYEAVCNGDTGHAEVVRVTFDPKLLSYRDLLEVFFVIHDPTTLNRQGNDVGTQYRS
                     AIFYHTPAQKAEAEQIISELVREKAFADPIVTEVVPAGQFYVAERYHQDYFQNNPNQP
                     YCHYVVAPKVAKFREKFARRLKKV"
BASE COUNT         2614 a         4642 c         4435 g         2838 t
ORIGIN      
        1 cccttctgcg tgatctcgat cggatcttcc gcagtccaga tctttttcct gccgttgttg
       61 atgtgcccga gcaccgaatg cagcgtggtg gtcttgcccg atccggtcgg gccgcagatg
      121 agcatcagac cgtaaggcat ggcggcgatg cgcttgattt cggcaagcac cggttcgcgc
      181 aggcccatct tgtcgagtgc gatcggcttg gccgcggcga gcacccgcat caccacgtcc
      241 tccaggccgt cggtcgtagg catgatcgcc acgcgcagtt ccacgttcgc cgtcccaaat
      301 cggttgaaga cgatcttgcc gtcctggggc ttgcgccgct ccgagatgtc gaggttcgac
      361 atgatcttga tgcgcgagat catcgcgttg cggaagtgcg cgggaacgtc gaggtagtcc
      421 tcgagttcgc cgtctatgcg cagcttgacc ttggtgtcct ctttgccccg ataagtctcg
      481 acgtgaatgt cggacgcctt gcgctcactg gcatcctgga tgatctggtt gacgatgcgc
      541 accagcgcgt tgtcggatac gccgacgccc ggatcgtcat gctctgcggc cttggccgcc
      601 gctggggcgg atgccgccag gtccttgacg ttgcgcgccg cttcgtactg gccgtagtac
      661 gttttgatgg cggagaggat gttttcctgg ttcgccatca ccgggacgac gcgcagccgg
      721 gtggagaagc gcaactcctg caccggcacc ggatcggtgg ggttctccat ggccaccacg
      781 atctcgtcct tggtccggaa cagcggtatg acgcgatgct tctcggcaag ccgccgttca
      841 accagcgcca gcgcacgctg ctcgatatcg aagcgcgtcg gatcgacgaa cggaatgccg
      901 agcttgcgga ccagcagccg gttcacgctg gcgcggtcga tcacgcccat ctcgatcagg
      961 atctcgccga gcggaagctt gcggtcgcgc tgctgcaggc tgaccgcctt gtcgagttcc
     1021 tccttgctga tgacctcttc gcgcaccagg acctggccga gctggctttc cggtagagtc
     1081 tggtcccggt ccttgagcac ctgctcgagg aactcgcggg agatgatctg gctggcggta
     1141 agataatcgc cgatgcgctg agtgcgcagg tgctgctgca gctgcagacc cacctcaacg
     1201 aagtccatcg tatccttccg ctgctcggta agcatctcgc cgagggactg gccgatggaa
     1261 tagtcggcat acgcatgcgt gggaatgaag gtgcgctgga tcttgccgtc ctccaccgta
     1321 agataaagat agaggccggt catctcgatc agaaacccgc gggtttcgcc ggcgagtttc
     1381 tcgccgtcct tgaacgaaac tttcacgatc tggttgccgg ttggtggcac gaggccggcg
     1441 ttctgcatct tctcgagttc cctcggcagc agttgcacgg ggcgcagcaa ctgcagcttc
     1501 ttgatcctgg cgaactgcag cgtctcgatc gaagtcagtc cggtggccac gaaatcgagc
     1561 gtcccctgtt cgggcagaaa gcggatgagc ctgccctgca tctgccggcc gtcgttgccg
     1621 acaagcaaac agctgcgggg aagtttcgcg tcctgggcgg acgcgtactg ataatagggt
     1681 ggtgtaggcc agacgaagct ttgttcttcg ggcatgagtc ctttctgtgg cgtaggccgg
     1741 cgcaggcggc attcccggag ccgctgcttt gcttccatag gatagaccag ttcggcggca
     1801 gacggtcaga cgcccgtggg caagccaatc cgggaagatg gccaagtcta tacttgcgca
     1861 tacgcccgat cttgcgggac aggccatgaa gacagtcgca ctttcgtcgg gtgaacgcgt
     1921 tcccgcgctc ggccagggca cctggtgcat gggcgagaac agggcggccc gcgccgagga
     1981 gatcgccacg ctcaggctcg gcctggatct tggcatcacg ctcatcgata cggcggagat
     2041 gtacggcgaa ggcaaggccg aggaactgat cggcgaagcg atcgccggcc ggcgcgacca
     2101 ggcgttcctg gtaagcaaga cctatccgca caacgccacg cgaaaaggcg cggtggcggc
     2161 gtgcgagcgc agcctcaggc ggctacgcac cgaccgcatc gatctttatc tgctgcattg
     2221 gcgcggtagc gtgccactcg ccgaaaccat ggaagctttc atggcgctgc agaaagacgg
     2281 caagatccgc cactacggcg tgagcaacct ggatctcgac gacatgcagg aattgcggtc
     2341 cgtgcccggc ggcaagggtg tggcgaccga ccagttgctc tacaacctcg cccgccgcgg
     2401 catcgagtgg gatctgttgc cgtggctgcg caagcggcac atcccggtga tggcgtattc
     2461 gccgatcgag cgcgccagcc tggcgcgcaa ctcgaaactt gcagatttcg cacgacgcca
     2521 cggcatgact ccagcgcaag ccgcgctcgc ctggctgctg gcgaaggacg acatcatcgc
     2581 cattcccaag accgctcatc gcgagcggtt gaaggagaat gccggcgcgc tcgaactaac
     2641 gctgtcgagc gaacagctgg ccaaactgga taatctcttt ccaccaccca aaggaccgcg
     2701 cccgctcgaa atgctctgag tgatggtggt aactgccagg aacgaaaatc aaggacgacg
     2761 cggaaaccga tgcgatcggg agttccgctg ccacgcgcaa ccccgatcca cagtctcagg
     2821 ctggcttcgc gagcggcagc ttgcgatggc gtttgccagt cagcgcgaag atggcattgg
     2881 ccactgccgg cgcgatcggc ggcgtgccgg gctcacccac cccgcccggg ttctcggcgg
     2941 atttcaccag atgcacttcg accacgggca tctcgttcat acgcaccacg gggtaatcgt
     3001 tgaagttgct ctgctcgacc ttgccgtcct tcagcgtgat ctcgccgtag agcgccgccg
     3061 agagaccgta aaccatcgcg ccttccatct gcgcgacgac ctggttcggg ttcaccacgg
     3121 tgccgacgtc ggcagcgatg acgacgcgat gcacgcgcgg ccgatcgtcc tccagcgaca
     3181 cctcggcgac ctgcgcaaca aagcttccga aagactcggc gaccgcgatg ccgcgcgcac
     3241 gcccctgcgg cagcggcgag ccccagcctg ccttgtcagc ggccaactcg agcaccgcct
     3301 tgtggcgcgg atgtttttcc agcagcgcgc gccggaactc gaacggatcc ttgccggcgg
     3361 catgggccat ctcgtcgatg aagctctcgc tgaagaagac gttctgggtg ctgcccaccg
     3421 agcgcaggaa ccaggtacgt acgccgggct gatacttcac ccagtcgacc ttcacattgg
     3481 cgatcgaata gggaatatcc gccagcccct cgaccgacgt gccgtcgatg cggtccttga
     3541 tgatcatggc ctcgaacccc gtgccttcgg cgagcgaatc gcacaccgtc gtcgcttcga
     3601 acgacaccgc tttgccttcg gcatccagcc cgcccttcaa cttcgcgatg gccatcggac
     3661 gatagtagta ggctttggtg tcgtcttctc tggagtagac cactttcacc ggcgctttcg
     3721 ctgctttcga caaactcacc gcctcgacca ggaagtcagg tgcaaagcgc cggccgaagc
     3781 cgccgccgag cagcaaagtg tggatcttca cctgctcggg cttgcagccg gcgatcatcg
     3841 ccgcagtcat ctggttgggt ccctgtgcct ggctgccgac ccacagctcc acgccgtcgg
     3901 gcttcaccca tgcggtgcag ttcatcggtt ccatcggcgc gtgcaccagg tagggtactt
     3961 cgtacagcgc ctccaccgtc ttcgctaccg tggccgccgc gacatcgcct tccttgcgtg
     4021 ccaggagcgc accctccccg gactcggcga acttcgacat ctgctcgcgc agacttgcgg
     4081 tcgaaagcgt cgcgttcggg ccggcgtccc actcgatctc gagcacatcg cggccgagct
     4141 ttgcggccca atagtcctgc gccagtacgg caacgccttc ggaggtcggg cttgtcaccg
     4201 gcagcaccat cttcacgcct ttgatcgctt tcgccttgtc ggcattgaag cgcacgagct
     4261 tgccgccgac caccggcgag cgcaccacga ccgcggtgag caggccgggg agcttggcgt
     4321 ccatgccgaa cttcgcgctg ccgttggtct tcgccggcgt atcgaggcgc ttgagcggct
     4381 tgccgatgat cttgaaagca gcggggtcct tgagcggcac ctccttcggc ggcgcgatgc
     4441 ccgccgcctt ggcggcgagc tgtccgtaag tggccctctt cccgcctggc ccgatcactg
     4501 cgccatactc ggtggaaagc ttcgcggggt ccaccttcca ggtctccgcc gcagccgcga
     4561 ccagcatcgc gcgcgcggtt gcgccggcct ggcgcagcgg ctgccacatc gcccggatcg
     4621 aagtggatcc gccggtgccc tgcacgccga accaggggtg tgcgtactcc tttgccgccg
     4681 gtgccggttc gatgcggatc ttcgaccagt ccacatcgag ctcttcagcg atcagcatcg
     4741 gcagcccggt gtaaacgccc tgccccatct ctgacttgtc gacgacgacc gtgacgatgt
     4801 tatccggcgt aatgcggatc cactggttcg gtgcgaaggt cttcccttcg gcggccagtg
     4861 cctgggtcgc aggcagcgcg caaccgatca caaatcctcc gccggcaagc gcggaaacct
     4921 tgatgaattc tcttcgattc atagccatgt gttcatctcc ttacactgag acgccggcgg
     4981 cctgtttgat cgcggcgcga atgcggccgt aagtgccgca gcggcagatg ttgccgctca
     5041 tggcggaatc gatgtccgca tcgcccggct ttggattcga tttcagcagc gcggccgcgg
     5101 acatgatctg cccggactgg cagtacccgc actgcggaac atcgagcgcc acccaggcgg
     5161 cctggatctt tctgcccgcc ggcgttctgc cgatcgcctc gatggtcgtg atcttcttgc
     5221 ccaccgccat cgaggccggc gtcacacagg atcggatcgg ctgaccgtcg aggtgcaccg
     5281 tacaggcgcc gcatagcgcc atgccgcaac cgaatttcgt tcctgtcata tcgaggtggt
     5341 cgcgcaacac ccagagcaag ggggtgcttt catcgacgtc gacctttctc gtcctgccgt
     5401 ttacgttcaa agcaagcatg cgacttctcc tttcgaggac aaaacttcgc ctgcacccgc
     5461 cggggcgggc atgcgcaact tttctagcgg ggatgcttga acccgagcat ataacttcga
     5521 gtcaactcca agtcaaggcc tttagcgcac cctggccgca ccaagcctcg aggtcgcatc
     5581 gcggctgaat ggagcgccgt gcggaatagg caccggttcc tatcgacagt cacgatcgaa
     5641 catagttgca attacgaacg ccgccctcct ttggggcggc aggcacccga gtagggtacc
     5701 tcggcgggga ccggcgatat tgacgccccg gaatgcctgc accgccaccg gaccggtctt
     5761 cgcgctgcac cgggatcgac cgatgagcca taatagaccg gcacggatgc gaggcgatgc
     5821 ggcatttttt ccggtgcgca gcagcacgtc tacgaacctg ggagcagcag ccatgaaacg
     5881 cggaaccggg ctcaacaatt tcgcgggcgc cgagggcggc acgccttcac tatcgcccaa
     5941 gattcgtgat gcgctcgcca agctcgacga tgcgctgttc gcaatctggg acctgggcga
     6001 caacgtgctg ctggcatgga cccacgagga cggcgagctg cgctttctcg cggttcgcca
     6061 ttacaccatc gccgagtcag tccacagcgg cgcggcgcat ccgaatcaga aaaaagatgc
     6121 catcggattc gtcaacgaga tcctcgccgg gtcaaagcac ctggcgccgg accggttccg
     6181 ggaagtgtgc cgcgagttcg gcgcggatcc ccagaccctg cgcgtggatt tccgggtcgg
     6241 caccgacgtt gacgtcgagc ggatcaacga gctggtgcgc cgctacagcg tctcgctagt
     6301 gcgcggcagg gcggtagtgc tgctggacgc ggtggagttc tcgctgcagc cgccgctcga
     6361 tcagatggcg atgctcaaca gcctgtcctt ttcggtgaac tcggcgtatc ggcaattgct
     6421 gtcgaaggag atcgacgtca atttcgcccg caccaccacc ggcgacggat tctatgtctg
     6481 gaatcgggcg acgaacccgg gcgccaacgt cgaactgtac aagctgatga tgctgatcct
     6541 cgccgacaac gcggtggcgc agcgaaaggc tcgtagcgaa tgggtgccga agttgcgggc
     6601 tgcatttcac gtaggcgagc actacgaatt ccaccaagtg gagggcctga acccgaccac
     6661 cttcagctac atcgtcggcc aggtcaccgt ggacctggcg cgcatgatcg agaagtcgct
     6721 gcccggccag atcctgctgg gcgacttcga agtcagggaa gaaatcaggc ccggcaggtt
     6781 cattcgctac gatacggtgg acttcgtcga gagaaccgcg gcgaccctcg atcacctcaa
     6841 cgggctcgtc atatccggcg gccgcatcga gaatatccgc tgctacctca cgggccagag
     6901 ccttggaagc ggccgataca tggtcaatcg ctatcacatc cgcgacaagc atgacaccag
     6961 ccgggtcgtt tacaacgcca agatcaatat ccatcgggaa aataccgaac ctatttttct
     7021 cggcatccag cgcgacgacc tgtgggcatt cgccaccgcc aaggtggaga cgttgaaccg
     7081 cgatgcggag cagacggggt cctatttcat caggccctga atccccgcca acgatccaac
     7141 gaaaacgggc gcctatcggt gcccgttttt tttgctgcaa tgctgcgagt accggtggcg
     7201 gcgtcagtcg aaactgaacc ccggcgtgcg cgtgtagtgg tgcaagccct cgtcctgtat
     7261 gaccagagag cgcatgcgtt tcacgccgcg gttgtggtgc gagtgcagca gcgtggccgg
     7321 cgtgatttgt gcggccccgg tggaccagtt cacgcgctgc ccgccgatca tcgagtaaat
     7381 gtcctcgccc tggatcgcga gcgtcaccgc cacgccgttg tgccggtgtg ggcgctgatc
     7441 ggcgccggga gcgagcgtat tgatcgccac gttgatcgaa gggatggtcg cgtagcccgg
     7501 cgccagcgcg gcggtcgaga acatcaccga atggccggtg gtattttcgg tgaccgggcg
     7561 ctgccagacc gcttcgaagc gccgctcgat ctcgtgcgca ggccagtgca ccgtatcgac
     7621 caccgactcc cgcggcgcag gcggacgcaa ccgctcgaag gccagcagcg gctcatcggt
     7681 cgccacgaac agcaggcagt cggcaccagc cgcgcggtgc gtggtttcgc tgccgccggg
     7741 aaagcagaac acatctcccg cgccccacgc gatcacgtcg gccgcattac ggctctcgcc
     7801 gcagcccgcc atgacgtagt agatcgcgcc ggaggccgca aaggctgcgc gcagatgttc
     7861 gctggcgcgg atcttcagat aacggcacaa aagcgttggc gaggtggccg gatacggcgt
     7921 gccgagattc ccggcagcat ccagcgcaat cgcaccggtc ggcaatgccg gatcgaaggc
     7981 ccggtcgcgt tcggccagga actgccgcgc cggtaccggc ggatagccga aggtgaacgg
     8041 gttttccgag gtgtggtagc gcgcgcggct cgcatattgc gtttgcggct cggcccgggt
     8101 gcggacaact tccttgacgg tcatggcgtc ctctttgtgt attgctctcg ccccataata
     8161 taccgcctgg agagaagttc caaaatcaaa gcactcaccg cggaagacgc agagcggcgc
     8221 acggacacgt taccgacgta gtcggccgtt tgcggggcgg ctgtgacacg ttagccgtag
     8281 gcccgcttgc gggagcagcc acatagcgcc ccgatccaca ccctgcaaca cagccttgtg
     8341 cgcccctacc cgcaaactgg gctacgccta acgagtccgg ggcgcacgcc gaagcgccgc
     8401 ctcccgtccc ggatggctac gccaacgccg tgtcggcggc gccgaggaaa aacaaaacga
     8461 ggaaaagcaa tattggaatt aaaagcgggg tgatcgatcg tcaacctcta tcggcacaat
     8521 tcaacgtggg gatcattcgc cgatgaatgc ctttagctcc agtgtgttct ctctcttgcg
     8581 tttcctccgc gtcctttgcg tccttcgcgg ggagagctat cagttgtttg cgggagcgcg
     8641 gccgggtccg ggtccggctc cggcatgaca aacggatgcc cggcttcata ggctcgcgcg
     8701 gcctgcagca cccgcgcatc ggcaaatcta tctcccacca attgcatcgc caccggcagt
     8761 ccttcgcgcg tgaatccgca cggcacggcg gcggccggct gctgcgagag attgaaggga
     8821 taggtgaagg gagaccattc ccaccagcgg gtccgcccgg agccaggtgg cacttcgtac
     8881 ccggcttcga aggccgccac cgcaagctgc ggcgtaataa gcaggtcgta gtcctggtgc
     8941 agcatgcaca tccggcgccc gaaggcctcg cgcgcgcgct cgagccgccg atattcgagc
     9001 gcgctcaggc caaaaccagg ctccgcgagt tcgagcaacg gcggatcgac gagcgcgcgt
     9061 tgcgccgtgg tcatgggcgc aatcgccagc gccagcgcga ccgcccataa cggctgcatg
     9121 attgcaatcg ggtcttcgag ttcgagatcg agctcctcga cccgtgcacc gagcgattcg
     9181 aaaacacgtg caccctcttc aaccagcatg gcaacctccg ggtccacgtg ggcgtaaccc
     9241 agagtggggc tgtagccgat gcgcaaaccc ttcacccccg aatcgagttc ctgccggtag
     9301 tcgatgttgc gtgccggcgc ctgataccag tcgcgcggat cggggcgagc gatcacggtg
     9361 agcatcagcg ccgcatcggc cacgttgcga gagatcggcc cttggtgcca aagtgttgcc
     9421 gccggtgaat gcggatgaac cggcacgatg ccaaaggtcg gcttgaagcc gaacagaccg
     9481 caaaaaccgg cagggatgcg gatcgaaccg ccgccgtccg tgccgatgtg cagcgagccc
     9541 atgccgaacg ctgccgcgac cgcggcgccg ccgctgctgc cgccgggcgt tagtcgcgta
     9601 ttccagggat tgacggtgat gccggtcaac ggactgtcgg tcacgccctt ccagccgaat
     9661 tcgggtgtag tggtcttgcc gagaaatacc gcgccctgct ccttcatgcg cgccactggt
     9721 ggaccgtctt ccgtccacgg ctgtttcgga tcaatggtgc gcgaaccacg cagtgtcggc
     9781 cagccttcgg tcacgataag atccttcact gtagtcggaa cgccgtccac gctgccggcg
     9841 ggcatgccgc gctgccagcg cttttcggaa gcctgcgcgt ctttgagtgc gcgagtctca
     9901 tcgaccatta caaaagcgtt gtaaagcgga ttgagccggg cgatgcgatc gagcgcggcc
     9961 cgtgtcgctt cgaccgggga aagttttttg ctgcggtaat gcgccgtcag ttcgacggca
    10021 gtcaatgaaa gaatgtcgtc ggacatctcg cgctcccact gcatgctgag aaaaaatgtt
    10081 tatgcggttt ttccgcaggt cgaaggttga attgcggcag gttcctgatc cgcatcgtca
    10141 gggcacactc ttcaaaggac atacaccgca aaggacgcca aggacgcaaa ggaagatcaa
    10201 gggggaaaag ttcgaaagtt aaaatcccta attggcgaac gatctaccaa agttgaatag
    10261 gcggaagagg gtcgcgaaca gtctcctcgc tcttaaccca aaattgcctt tcctcgttct
    10321 tgtctttcct tcgcgtcctt ggcgtccttg gcggtgagtg cttattgtgt attacgccgc
    10381 gtcgcagacg cagacacaag gagcgcccgg ccccgcggca tttgccaccg cgaggccggg
    10441 ccggcttttt tcggccggtt cgctacgcct tctgcttcat cgctgccagc actgcctcgg
    10501 gcgagatcgg catatgcctt acgcgcgcgc caactgcatt ggcgatcgcg tttgcgaccg
    10561 ccggcgccac gacggttgac gccggttcgc ccacgccggc cggatgatgt ttcgactgaa
    10621 tgatcgtgac atcgatctcc ggcacctgcg ccaggcgtat cggctggtac tcgtggaagt
    10681 tgctctgctc gaagctgccg ttcttcagcg tcagtttttc cgacatgatc tggctcgccc
    10741 cccagagcgc cgagccgcgg atctgtgcct cgatgttgcc cggattgacg gcgatgccgg
    10801 gatccatggc gatggtgatc ctgttgatgc tgtacctgcc ggtcgccgga tcgacggtca
    10861 cctccgccac gccggccacc caggtcgggc tctgacgttc ttccgccgca gtgaccgcga
    10921 tgcccattcc ggtattcggc ggaagcttct tggtgccgta gcccgacttg ccggctgcaa
    10981 cgcgcaggca gttggcaaga cgcagcgcgc cgccaaccgt gcccgactcg tacaccggcc
    11041 aggacccttc cttcgcccaa ggcaacgaga tccacaactg gtccatgtag tagtcggagc
    11101 cggtgccggc cggatagccg ctgttgggta tgccgcggtt gccgcctttg ccgttcaaca
    11161 tcgccagccg gaactcgagc ggatcgcgcc ccgccgcacg cgccacttcg tccatgaagg
    11221 actcgacgac gaagatgttg taggagttgg acaccgtgcg cagcgcgctg gcctggaccg
    11281 cccaggtggt aggctcgtgg ttccaggcgc gcacgcggtg gttcttcacg ttgtaccaat
    11341 ggtcgctgcc gccgattgac cattggtcga tgtcctgctt cttggcatcc caggaatcga
    11401 gttgcagcca gtcggtcccg ccagccttgc cgacgccaaa tcgcgccccc atccagccgc
    11461 agacgatgtc ctggttcatc gccgtcagtt cgccgttttt gatgccggcc ttgaccttgt
    11521 ggtaggtcgg cgtgcgggca aaactggttg cgaaattgga ttcgcgcgta tggatgagct
    11581 tgaccggacg gccggtttcc ttgacgcagt aagctgccgc caggatgtcg tcgtagtcct
    11641 gcttgccgcc gaacccgcca cccaccagat actggtgcac gaagaccttg atgtcctctg
    11701 gcttcttttt cagcacattg gaaagatagc cggtcaaggt catgcgcgcg aaagacgtgc
    11761 tctgcgtgcc aacatggacg tgccattcgc cgttggtcag ctgcactgtt gcattcaacg
    11821 gctccatggt ggcgtgacag accatatcgg tcgtgtattc cacctccagt actttctcgg
    11881 catcaccgaa acccttgtcg acatcgcctt cgagcaccca gttcgcacct tgcttctcgt
    11941 ccttcgcaag ttgcttgtac tgctcgagca tttccgccga actcaggttc gcgtaagggc
    12001 ccgcgtccca tttcaccttc agcgccttgg cggctttcat tgcggtcgga aacttgtcgg
    12061 cgacggcaac cacccagcca gtgcattttc ccatcgcatt gtcgatcttg accgccttga
    12121 tgaaaccggg gatcttcttc gcttccgaat cgtcgatgct caacactttc gacgcataac
    12181 ggctgcgcgg cacgaccagc gcaccgtaag ccatgttcgg caggaagacg tcgatgccgt
    12241 atttggcttt gccgttggtc ttcgccggga tatcgagcgc agcaaacgac ttgccgataa
    12301 tcttgtactg gctcggatct ttcagcgtga tgcccttgag atcgtccggg taggcgaact
    12361 ttcgatcgat cttcaccttc tgcaggatct cgccgtaacc gaccgatctg ccggaggcct
    12421 tgtcgaccac gcgtcctgct tccgcatggc agtcggctag cttggcgttg agcactttgg
    12481 cgccggcttc caccagcgcg atgcggcccg cggcgccggc gcgcgcaata cgatcgaact
    12541 cggtcgtgac gctgccgctg ttgacggtgt aggccaatcc gtagacgccg aaattctcca
    12601 cgctttccag cggcgcatcc aggcgcacgt cttcccatct gacttccagt tcttcggcaa
    12661 tcacctgtgc caggccggtg ccgatgtgct ggcccatttc cgccttgacg atgtgcatcg
    12721 tggtcttgcc gtccggcgtg atggtgaacc agatcgccgg cgtaaaggcc ggcgtggcca
    12781 tagccgcttc ggcgttcttg ccgcccagca gcgccggcat gccaatgctg aaaaagccgc
    12841 tgccggcgat cactgtcgag cggaccagga attcgcggcg cgtcattgcc tgcgtttggg
    12901 tctcagccgt gattggctta accatgattg ctgccctcct tcatcagatc ggcggcgcga
    12961 tgtaccgcac cgacgatgcg ggaatacgta gcgcagcggc acaggttgcc gttcatgtgc
    13021 gacacgattt cttcgctggt gggattggga ttgacggcga gcagcgcggc cgcttgcatg
    13081 atctggccgg actggcagta gccgcattgg ggaacctgcg cttcgatcca ggctttctgc
    13141 accgggtgtg catcggccgg atcgagcccc tctatggtgg tgatcaccgc gttgctctcc
    13201 acgtcgccga gcttgaccat gcatgagcgc acgggctggc cgtttatgtg cacggtacag
    13261 gaaccgcaaa ccccgagacc gcatccatac ttggtgcccg tgagtcgcag atcctcgcga
    13321 atggcccaga gaagtggcgt gtcgggcggc gagtcggtga aataactttg accgttaatg
    13381 ctgagttgtg gcatggctgc gagacctccc ttgcaagtta cgatgacaaa atggccccta
    13441 cgaatgctgc aggccaatcc agagcgaaac gactgcgacc cgcaatgcca ggctttcccc
    13501 cgactgtcgc gacgtcgcgc ggtttcccct tacccgtgag cggagcattg acacacttcg
    13561 tctccgccgt gttgcccagg cgcaaacatg agcatgcgcc ccggcatgaa tgccgatcaa
    13621 tcgaaatcat ttgtaaggca ggcgctggcc caccgtgaaa ttgccgaagg aagcctcggc
    13681 ggtacagtac cactggtact gcgcataggg gaactccgtc cacgactggt ataagcgatt
    13741 atgcacgcac ctttgataca acactccccg atacagacgc cggcaggcgc tgcaagacga
    13801 tgcggcctca caccttcttc agccgtctgg cgaacttctc cctgaacttg gccactttcg
    13861 gggccaccac gtagtggcag tacggctggt tcggattgtt ctggaagtaa tcctggtgat
    13921 agcgctcggc gacgtagaac tgcccggccg gtacgacttc agttacgatc ggatccgcaa
    13981 aagctttttc ccggacaagt tcgcttatga tttgttcggc ctcggctttc tgcgccggtg
    14041 tgtggtagaa aatcgccgag cggtactggg tgccgacgtc gttgccctgg cggttgagcg
    14101 tggtcggatc gtggatcacg aagaagactt ccagcaggtc gcggtagctg agcaacttcg
    14161 gatcgaaagt aacgcgaacc acctccgcgt gcccggtgtc gccattgcac accgcttcat
    14221 aggtcggatc gggtcgttgg ccgcccatgt aaccggactc cacgctctcc acgccctgca
    14281 gccggtcgta caccgcctcc aggcaccaga aacacccgcc cgccagcgtc gcgatttctt
    14341 gaccttcccg agcctgttcc atcgccgacc tccacgattg cctaattaac agtgtagcgg
    14401 catcgcgagt ggtcgagggc agtcgttggg tcttacgtcc cgaccctcat gccggacagg
    14461 aagcggcacg ggcgggctgg tcggtggcta cgacttcctg gtacgtggcg aacagttttc
    14521 gcgccagcg
//
LOCUS       DASQOP010000356         5823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2403168, whole genome shotgun sequence.
ACCESSION   DASQOP010000356 DASQOP010000000
VERSION     DASQOP010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5823
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2403168"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VE030_13780"
     CDS             <1..363
                     /locus_tag="VE030_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HZF24524.1"
                     /translation="KILGPVTIGDGAKVGSNAVVVKDVPARATAVGIPARILDDETMA
                     EREQTAERMGFSAYGIGPDTNDPVVKAIHGLIDHTVSADRRIEYILEQLARMGVKVED
                     ARATADKFDPAYLNRIVD"
     gene            446..931
                     /gene="iscR"
                     /locus_tag="VE030_13785"
     CDS             446..931
                     /gene="iscR"
                     /locus_tag="VE030_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HZF24525.1"
                     /translation="MRLTTKGRFAVTAMIDLAMRNGEGPVTLADISDRQKISLSYLEQ
                     LFGKLRRQGLVSSVRGPGGGYNLARPTQEVSVADIIVAVDEPIDATQCGGKENCKDEQ
                     KCITHDLWADLNKHIFDYLGAVKLSELVAKQTKKSSDVAVVQMHDSRQNVAKPKEPAS
                     V"
     gene            942..2084
                     /locus_tag="VE030_13790"
     CDS             942..2084
                     /locus_tag="VE030_13790"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HZF24526.1"
                     /translation="MHVYFDHNATTAPDERVLEAMLPFLKGEYGNASSRHEFGTRARK
                     AVSRAREQVARLVNVQGSQVVFVSGGTEANNLFIKGAAAYLKSTQVVVSGVEHPCVAK
                     PAQDLARQGWKLRKIAVRPNGLVDLADVDAALTDPTGLVSVMLANNETGVIQPVAEIA
                     AKARAKRAWMHTDAVQALGKIPVDFEALGVHAMTISAHKIYGPKGVGALVVDKRLELK
                     PIIHGGGHEMGLRSGTENVPAIVGFGSACELAAERMDKISRKLQELRERTDQGVSALG
                     AVIFGQGAPRVPNTTYFAAPNIDGETLVIELDKAGFAVASGAACSSTSTEPSATLLAM
                     GVAPELARGAVRVSFGVHNTAAQVEDFLQALSAVLKRLRRLTAIAV"
     gene            2115..2438
                     /locus_tag="VE030_13795"
     CDS             2115..2438
                     /locus_tag="VE030_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HZF24527.1"
                     /translation="MAITVTENAAKHIRGALAKRGGVGLRFGVRKVGCSGFAYTFDYA
                     QEVNADDQLFQSHDITVVVDKDSLEFVDGAVLDYAREGLNESFKVENPKAKAMCGCGE
                     SFSVA"
     gene            2465..3898
                     /gene="sufB"
                     /locus_tag="VE030_13800"
     CDS             2465..3898
                     /gene="sufB"
                     /locus_tag="VE030_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HZF24528.1"
                     /translation="MSAVIQNLVNQPYKHGFVSDIESDVVPKGLSEDVIRLISAKKGE
                     PEWLLEFRLKAYRHWLTMQEPTWPNVKYPKIDFQDISYFSAPKPKKLASMDEVDPELL
                     RTFEKLGVPMHERKTLAGVAVDVIFDSVSVATTYKKKLAEVGVIFCSFSEAVKDHPEL
                     VKKYLGSVVPTGDNFYAALNSAVFTDGSFCYIPRGVRCPMDLSTYFRINTQESGQFER
                     TLIIAEEGAYVSYLEGCTAPKFDTNQLHAAVVELIALDDAEIKYSTVQNWYAGDENGK
                     GGIYNFVTKRGLCKGTNSKISWTQVETGSAITWKYPSCVLLGDNSAGEFYSVALTNHH
                     QQADTGTKMIHIGRNTRSKIISKGISAGVSNNSYRGLVKIGARAEGARNFSQCDSMLI
                     GDRCGANTFPYIQVNNNSAKVEHEASTSKIGEDQLFYFASRGIGNEEAVSMIINGFVQ
                     DVFVQLPMEFAVEATKLLGLKLEGSVG"
     gene            3895..4668
                     /gene="sufC"
                     /locus_tag="VE030_13805"
     CDS             3895..4668
                     /gene="sufC"
                     /locus_tag="VE030_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HZF24529.1"
                     /translation="MIRENTALLLEVRGLTARIGDRNILKGIDLTVRAGEVHAIMGPN
                     GSGKSTFAKVLSGHPSYEVTGGEVLFEGRNLFELAPEERARAGLFLGFQYPIEVPGVT
                     NSAFLRLAYNTVQGTRGKEELDPLEFDDLVREKMKLLDIDPSFLGRSLNAGFSGGEKK
                     RNEILQMALLEPKLAVLDETDSGLDIDALRVVANGINKLHTPDNGMVLVTHYQRMLNY
                     IVPDYVHVMEGGRIIRTGDKALALELEARGYDWVGKEAA"
     gene            4679..>5823
                     /gene="sufD"
                     /locus_tag="VE030_13810"
     CDS             4679..>5823
                     /gene="sufD"
                     /locus_tag="VE030_13810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HZF24530.1"
                     /translation="MNTETHSDYLDSLLHGRDLVPEARAGWLKALRAQAVERANALTV
                     PTTRDEDWRFTDLSPLYKVPYRPSAQPGQIAAAEFGRFEIPEAATRLTFVDGNFSAAL
                     SRMKPVDGVFVANLPDALAARDRLVESRLGKLAGTGQDAFAAVNTAFLHDGALIHAGR
                     DHKGIAPIHLLFIATRKDGAAYPRVLVIAEPGAQCTLIEDFVGLADGGYFTDAVTEIA
                     VGENAHVAHVKLQREAQTAFHIARTSVQVARDGHYSNWSVALGARISRHNLHVMQAGE
                     GSEFSIDGLALIGGRQLADTNSLIDHAHAHGRSRQLHKTVVGGGAHAVFNGKIFVRRD
                     AQHTDAGQQSRNLLLSDKAHVDTKPQLEIFADDVKCAHGATVGQIEA"
BASE COUNT         1222 a         1673 c         1890 g         1038 t
ORIGIN      
        1 aagattctcg gcccggtgac gatcggcgac ggcgccaagg tcggttcaaa tgccgtggtg
       61 gtgaaggacg taccggcacg cgcgaccgcg gtcggtatcc cggcgcgaat cctggacgac
      121 gaaaccatgg ccgagcgcga acagaccgct gaaaggatgg ggttttctgc ttacggcatc
      181 ggcccggaca cgaacgatcc ggtggtgaaa gcgattcacg gactgatcga tcacaccgtg
      241 tccgcagacc ggcgcatcga atacatcctg gagcaacttg cccgcatggg cgtcaaagtc
      301 gaggacgccc gagccaccgc agacaagttc gatcctgcct acctgaaccg cattgttgac
      361 taaagcagtc gggtttcagt atacttgagc gtgttagtag gatttataag ctgctgtttt
      421 tatgaattat tgagcagagg cgaccatgcg actcacgacc aaagggcgtt ttgcagtaac
      481 cgccatgatc gatctggcga tgcgcaacgg agaaggtccg gtgacgctgg cggacattag
      541 cgacaggcag aagatttcgc tgtcctacct cgaacagttg ttcggcaagc tgcgcaggca
      601 ggggctggtg tccagcgtgc gtggtccggg aggcggctac aacctggcgc ggccgaccca
      661 ggaagtttcg gtggccgaca tcatcgtcgc ggtggatgaa cccatcgatg cgacgcaatg
      721 cggcggcaag gagaactgca aggatgagca gaagtgcatt acccacgatc tgtgggccga
      781 cctcaacaag cacatcttcg actacctcgg cgcggtgaag ctctccgagc tggtggcgaa
      841 gcagacgaaa aagtcctccg acgttgcagt cgtgcagatg cacgacagcc gccagaacgt
      901 ggcgaagccg aaagaaccgg cgtcagtctg aactgaacgc gatgcacgtc tatttcgacc
      961 ataacgccac cacggccccc gacgagcgcg tgctcgaggc gatgctgccg ttcctcaaag
     1021 gcgagtacgg caacgcctcc agccgtcacg aattcggcac ccgcgcgcgc aaggcggtga
     1081 gccgggcgcg cgagcaggtc gcaagactgg tgaacgttca aggctcgcag gtggtattcg
     1141 tgtccggcgg gaccgaggcc aacaatctgt tcatcaaggg tgcggcggca tatttgaagt
     1201 caactcaagt tgtggtgagc ggcgtcgagc atccctgcgt ggcgaagccg gcgcaggacc
     1261 tggcgcgtca gggctggaag ttgcgcaaga ttgcggtccg gcccaatggg ctggtcgatc
     1321 tcgccgacgt ggacgcagca ctcactgacc ccaccggact ggtgtcggtc atgctcgcca
     1381 acaacgagac cggcgtgatt cagcccgtgg cggaaattgc cgcaaaggcg cgcgcgaagc
     1441 gcgcctggat gcacaccgat gcggtgcagg cgctgggcaa gatcccggta gatttcgaag
     1501 cgctgggcgt gcacgcgatg acgatatcgg cgcataagat ctacggcccg aagggtgtcg
     1561 gtgcgctggt ggtggacaag cggctggaac tgaagccgat catccacggc ggtggccatg
     1621 aaatgggact gcgctccggc acagagaacg tgccggcgat cgtcggattc ggcagcgcct
     1681 gcgaactcgc ggcggagcga atggacaaaa tctcgcgcaa gttgcaggag ttgcgcgaac
     1741 gtacggatca aggggtgtcg gcattgggtg cggtgatttt cggccagggc gcgccgcgcg
     1801 ttccgaatac cacgtacttc gccgccccca acatcgacgg ggaaaccctg gtgattgagc
     1861 tggacaaggc cggcttcgcg gtggcgagcg gcgccgcctg ctccagcacc agcaccgaac
     1921 ccagcgcgac gctgctggcg atgggcgtgg cgccggaact ggcgcgcggt gcggtgcggg
     1981 tgagcttcgg cgtgcacaac accgcggcgc aggtcgagga ttttctacag gcgttgtccg
     2041 cggtgctcaa gcggctgcgg cgcctgacag caatcgcagt ttgaattcgg caaccagtaa
     2101 agggtgaatt gatcatggca attactgtta cggaaaacgc ggcgaagcat atccgcggcg
     2161 ctttggcaaa gagaggcggc gtgggccttc gcttcggcgt caggaaagtc ggttgctcgg
     2221 gctttgccta taccttcgac tacgcgcagg aagtcaacgc cgacgaccag ttgttccagt
     2281 cccacgacat caccgtggtg gtggacaagg acagcctcga gttcgtggac ggcgcagtgc
     2341 tcgactatgc gcgcgaaggg ctgaatgagt ccttcaaggt cgagaacccg aaggcgaagg
     2401 cgatgtgcgg ctgcggcgaa agcttcagcg tcgcctgacg aagataattt caccggagcg
     2461 gtttgtgagc gcggtcattc agaacctggt taatcaaccc tacaagcacg ggttcgtttc
     2521 cgacatcgag tcggacgttg tgccaaaggg gttgagcgag gacgttatcc gcctgatttc
     2581 ggcgaaaaaa ggcgagcccg aatggctgct agagttccgc ctgaaggcgt atcgccactg
     2641 gctgaccatg caggaaccga cctggccgaa cgtcaaatac ccgaaaatcg acttccagga
     2701 cataagctat ttctctgcgc cgaagccgaa aaagctcgcg tccatggacg aagtggatcc
     2761 ggagttgctg cgcactttcg agaagctggg cgtgccgatg cacgagcgca agacgctggc
     2821 cggcgttgcg gtggatgtga ttttcgacag cgtgtcggtc gccaccacgt acaagaagaa
     2881 gctggccgaa gtcggcgtca tcttctgttc cttttccgag gcggtcaaag accatcccga
     2941 gctggtgaag aagtatctgg ggtccgtggt gccgaccggc gacaattttt atgccgcgct
     3001 caattccgcg gtgttcaccg acggctcgtt ctgttacatc cccaggggcg tgcgttgccc
     3061 gatggatcta tccacatact ttcgcatcaa tacccaggag tcgggccagt tcgagcgcac
     3121 gctgatcatc gcggaggagg gcgcttacgt gtcctacctc gaggggtgta ccgcgccgaa
     3181 gttcgacacc aaccagctgc acgcggcggt ggtggaactg attgcgctgg acgatgccga
     3241 gatcaagtat tcgacggtac agaactggta cgcgggagac gagaacggca aaggcggcat
     3301 ctacaatttc gtcaccaagc gcggtctgtg caagggaacg aactcgaaga tttcctggac
     3361 ccaggtcgaa accggttcgg cgatcacctg gaagtatccg tcgtgcgtgc tgctgggcga
     3421 caactcggcg ggcgaattct attcggtggc gctgaccaac caccaccagc aggccgatac
     3481 cgggaccaag atgatccata tcggcaggaa cacccgcagc aagatcattt ccaagggcat
     3541 ttccgctggc gtttcgaaca acagctatcg cgggctggtg aagatcggcg ccagggccga
     3601 gggcgcgcgc aatttctcgc aatgcgactc gatgctgatc ggcgaccgct gcggggcgaa
     3661 cactttcccg tacatccagg tcaacaacaa ctcggcgaaa gtggagcacg aagcctcgac
     3721 ttccaagatc ggcgaggacc agctttttta tttcgccagc cgcggcatcg gcaacgagga
     3781 agcggtgtcg atgatcatca acggtttcgt gcaggacgta ttcgtacaac tgccgatgga
     3841 gttcgcggtc gaggcgacca agctcctcgg actcaagctt gaaggaagtg tcggatgatc
     3901 agggaaaaca ccgccctgct gctggaggtc cgcggcctca ccgcaagaat cggcgatcgc
     3961 aacatcctca agggaatcga tctgaccgtg cgtgccggtg aagtgcacgc gatcatgggg
     4021 ccgaacggct cgggcaagag cacgtttgcg aaggttctga gcgggcatcc gtcgtatgaa
     4081 gtgaccggcg gcgaagttct gttcgaaggc aggaacctgt tcgaactggc gccggaagag
     4141 cgtgcccgcg ccggtctgtt cctgggcttc cagtatccga tcgaggtgcc tggcgtcacc
     4201 aacagcgctt tcctgcgact ggcctacaac actgtgcagg gtacgcgcgg gaaggaagaa
     4261 ctggatccgc tcgagttcga cgacctcgtg cgcgagaaaa tgaaattgct ggatatcgat
     4321 ccgtcttttc tcgggcgcag cctgaacgcc ggcttttcag gcggtgagaa gaagcgcaac
     4381 gagattctgc agatggcgct tctggaacca aagctcgcag tgctggacga gaccgactcg
     4441 ggcctggaca tcgacgcgtt gcgggtggtg gcgaacggca tcaacaaact gcacaccccc
     4501 gacaacggca tggtgctggt gacgcattac cagcgcatgc tcaactacat cgtgccggat
     4561 tacgtgcacg tcatggaagg cgggcggatc atcaggaccg gcgacaaggc gctcgcgctc
     4621 gagctcgaag cgcgcggcta cgattgggtg ggtaaagagg ccgcgtgagt ttgatcgcat
     4681 gaataccgaa acccacagcg actatctgga cagcctgcta cacggccggg atttggtgcc
     4741 cgaagcccgc gcaggatggt tgaaggcgct gcgcgcccag gcagtggagc gcgccaatgc
     4801 gctcactgtg ccgaccacgc gcgacgagga ctggcgcttc accgacctgt caccgctgta
     4861 caaggtcccg taccggccat cggcgcagcc aggccagatt gcggcggccg aattcggccg
     4921 tttcgagatc cctgaagcgg cgacgcggct gactttcgtg gacggtaatt tttcagctgc
     4981 gctgtcgcgt atgaagcctg tagacggcgt tttcgtcgcc aatcttccgg atgcgcttgc
     5041 ggcacgcgac cggctggtcg agtccaggct cggcaagctt gcgggaacag ggcaggacgc
     5101 tttcgccgca gtgaatacgg cgtttctaca cgacggcgca ctgatccacg ccgggcgcga
     5161 ccacaaaggc atcgcaccaa ttcatctgtt gttcatcgcc acccgcaagg atggcgccgc
     5221 ctatccgagg gtcctggtaa tcgcggaacc gggtgcgcag tgcacgctga tcgaagactt
     5281 cgtgggcctt gccgacgggg gttactttac cgacgcggtc accgaaattg ccgtgggtga
     5341 gaatgcgcat gtagcgcacg tcaagctgca gcgcgaggcg cagacagcat ttcacatcgc
     5401 caggacttcg gtacaggttg cgcgcgacgg ccattactcc aactggtcgg tcgcgttggg
     5461 tgcgcgcatt tcccggcaca atctccacgt gatgcaggcg ggcgagggta gcgaattctc
     5521 gatcgacggc ctggcgctga tcggcgggcg ccagttggcc gacaccaaca gcctgatcga
     5581 ccacgcgcac gcgcatggac gcagccggca attgcacaag accgtggtcg gcggcggcgc
     5641 gcacgcggtg ttcaacggca agattttcgt gcgccgggac gcgcagcaca ccgatgcggg
     5701 gcagcaatca cgcaacctgc tgctgtccga taaggcgcac gtggatacca agccgcaact
     5761 cgaaattttt gccgacgacg tcaagtgcgc gcacggcgca accgtcggcc agatcgaggc
     5821 cga
//
LOCUS       DASQOP010000357         5139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1195356, whole genome shotgun sequence.
ACCESSION   DASQOP010000357 DASQOP010000000
VERSION     DASQOP010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5139
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195356"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /gene="xth"
                     /locus_tag="VE030_13815"
     CDS             complement(<1..713)
                     /gene="xth"
                     /locus_tag="VE030_13815"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HZF24531.1"
                     /translation="MKLAAWNVNSLKVRLPHLLEWLARERPDAVCLQETKVEDANFPL
                     DAIAQAGYRAVFSGQKTYNGVAILAHDVPLAVEAGISGYADVQKRVLSATVGDLRLVC
                     AYVPNGQSPESDKYRYKLDWLAAFDRWLDAELKKHPRLAVLGDFNIAPEDRDVHDPKA
                     WEGQVLVSPPERAAFQRLLGHGLKDSFRLFDQPEKSYSWWDYRMNAFKRNLGLRIDHV
                     LLSPELASRCRSSSIHVDMR"
     gene            complement(720..1289)
                     /locus_tag="VE030_13820"
     CDS             complement(720..1289)
                     /locus_tag="VE030_13820"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HZF24532.1"
                     /translation="MKQWILLMVLMPGAVLAQTETYKWVDADGTIHYSDQPPPPSARI
                     SERKRLGDKPGDVAMPYVLQQAMKNFPVTIFVYDCGDGCDRARALLGKRGVPHTVKDP
                     LAPGMREELKEATGGDEVVPVLQVGRQVLRGFEEGQWNAALDDAGYPKTALVPVTPVQ
                     ARPVAAAPPAAPEEAAPKSGSEPAKPEQN"
     gene            complement(1318..3348)
                     /locus_tag="VE030_13825"
     CDS             complement(1318..3348)
                     /locus_tag="VE030_13825"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HZF24533.1"
                     /translation="MNPLLDFSGLPRFADFKPEHVTPALDQLLAANRELIARQEADRR
                     EPSWENFVAPLDDANEQLRRAWGQVAHMNAVMNSPALREAYNANLGRITQYFTELSQS
                     EALFRKYKALRASPGFSALTQAQRKVIDNELRDFRLGGAELPAERKARFKQVTERLAK
                     LSSEFNDNLLDATNAFELIIQDRAQLSGIPDDVLEAARKAATEIGKTGWKLTLQMPSY
                     LPVMQYADNRELRERMYHAYATRASEFGNPAWDNSALISEILKLRREQAQLLGFKTFA
                     ELSLQPKMADSPAQVLRFLDELATRAKPYAERDLAELEEFAARQLGLAELQPWDVAYG
                     SEKLRVARYAFSDQEVKQYFPEQQVLAGMFRVVETLYGLGIAADDAPRWHEAVRFFSI
                     RNADGALVGQFYLDLYARPSKRGGAWMDDAITRRRKAAGIQTPVAYLNCNFSAPVGGR
                     PAMFTHDEVITLFHEFGHGLHHLLTRVDVLGVSGINGVEWDAVELPSQFMENFCWEWE
                     VLKDMTRHVDSGNSLPRALFDKMVAAKNFQSGLQTVRQLEFALFDMHLHHDFEPVGTK
                     TPLDLLDEVRSRVAVIIPPAYNRFPHGFSHIFGGGYAAGYYSYKWAEVLSADAYSLFE
                     ESGVLNPVVGERFREEILAAGGSRPALESFVAFRGREPKIDALLRHNGMAVA"
     gene            complement(3450..4724)
                     /locus_tag="VE030_13830"
     CDS             complement(3450..4724)
                     /locus_tag="VE030_13830"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HZF24534.1"
                     /translation="MKIHIKNGRVVDPQSGTEKVQDLYLAAGKIAAIGRAPDGFHANR
                     VIEANGLVVSPGLVDLSARLREPGFEHKATLESEMAAAVAGGVTSLACPPDTDPPLDE
                     PGLVEMLKHRARSLNQAHVYPLGALTLGLKGERITEMAELVDAGCVAFSQAEVPLDDN
                     QVMLRAMQYATTFGYSVWLRPSDGSLARGGVAHDGQVATRLGLAPIPVAAETVALSTM
                     LLLARETGARLHLCRLSSAEGVEMMRQAKALGMNVTCDVSANHVHLSEMDIGYFDSNY
                     HLVPPLRSLRDRDALRRGVEDGTIDAICSDHTPVDEDAKQLPFGESEAGATGLELLLA
                     LTLKWSEESRIALPKAIAKVTTEPARILGIGAVAIAAGANADLCIFDPHLRWKVEPAR
                     LKSQGKNTPFAGYEVNGRVRYTLVGGHVVYEC"
     gene            complement(4942..>5139)
                     /gene="pyrB"
                     /locus_tag="VE030_13835"
     CDS             complement(4942..>5139)
                     /gene="pyrB"
                     /locus_tag="VE030_13835"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454302.1"
                     /note="catalyzes the transfer of the carbamoyl moiety from
                     carbamoyl phosphate to L- aspartate in pyrimidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HZF24535.1"
                     /translation="KYYGLTPEKLAGAKPDAIVMHPGPMNRGVEIDSSVADGNQSVIL
                     PQVTFGIAVRMAVMSILAGNA"
BASE COUNT          934 a         1682 c         1541 g          982 t
ORIGIN      
        1 cgcatatcca cgtgaatgct gctcgagcgg cagcgcgacg cgagttcggg agacagaaga
       61 acgtgatcga tgcgcaaccc cagattgcgc ttgaaagcat tcatgcggta gtcccaccag
      121 gagtatgact tctcgggctg gtcgaacagc ctgaagctgt ctttcaggcc gtgcccgagc
      181 agccgctgaa acgcagcacg ctcgggcgga ctgaccagaa cctgaccttc ccaggccttc
      241 gggtcgtgga catcgcgatc ttccggagcg atgttgaaat cgccgagcac cgccagccgt
      301 gggtgcttct tcagttccgc gtccagccag cggtcaaatg ccgccagcca atcgagcttg
      361 tagcgatact tgtcggattc cgggctctgg ccgttgggca cgtacgcaca taccagccgc
      421 aagtctccca cggtggcgga gagcacacgc ttctgtacgt cggcgtagcc ggagatgccc
      481 gcctcgaccg ccagcggcac atcgtgcgcc aggatcgcca cgccgttgta ggttttctgc
      541 ccggagaaca cggcgcggta tccggcctgt gcgatggcgt cgagcggaaa atttgcatcc
      601 tcgactttgg tctcctgcag gcaaaccgcg tcggggcgct cgcgcgcgag ccactcgagg
      661 agatgcggaa ggcgaacctt cagcgagttg acattccacg cggcgagctt caagcgtcgt
      721 cagttctgct cgggcttggc cggctcgctc ccgcttttgg gcgccgcctc ctccggcgcc
      781 gccggcggtg cggctgccac cggcctggcc tggaccggcg tgaccggcac caacgcagtc
      841 ttggggtagc cagcgtcgtc gagcgcggcg ttccattgcc cttcctcgaa gccgcgcaag
      901 acctggcgcc ctacctgcag caccggcacc acctcatctc caccggtcgc ctctttcagt
      961 tcctcccgca tcccaggcgc cagcggatcc ttgacggtat gcggcacgcc gcgcttgccg
     1021 agcagcgcgc gggcgcggtc gcagccgtct ccgcagtcat acacgaaaat cgtcaccggg
     1081 aaatttttca tcgcctgctg gaggacgtag ggcatcgcga catccccggg cttgtctccg
     1141 agtcgcttgc gctcgcttat gcgggcgctc ggaggcggcg gctgatcgct gtaatgaatc
     1201 gtgccgtccg cgtccaccca tttgtaagtc tcggtttgcg ccagcaccgc acccggcatc
     1261 aagaccatga gaaggatcca ctgtttcatc caggcattct cctttccatt gccggcatca
     1321 tgccacggcc atgccgttat gccgcagcaa cgcatcgatt ttcggctcgc gcccgcgaaa
     1381 cgccacgaag gactcgagcg cggggcggct gccgcccgcg gcgaggatct cttcgcggaa
     1441 gcgctcgccg acaactgggt tcaacacccc gctttcctcg aacaggctgt aagcgtctgc
     1501 ggacagtacc tccgcccatt tgtagctgta atatccggcc gcatacccgc cgccgaagat
     1561 atgcgaaaag ccgtgcggaa agcggttgta cgcgggcgga atgatgacgg cgacccggct
     1621 gcgcacttcg tcgagcagat cgagcggcgt tttcgtaccc accggttcga agtcgtggtg
     1681 cagatgcatg tcgaacagcg cgaactcgag ctggcgcacg gtctgcaggc cgctctgaaa
     1741 attcttcgcc gccaccatct tgtcgaaaag cgcgcgcggc agactattcc cgctatcgac
     1801 gtggcgggtc atgtccttga gtacctccca ttcccagcag aagttctcca tgaattggct
     1861 gggcaattcg acggcgtccc actcgacacc attgattccc gacacgccga gcacgtccac
     1921 ccgggtcagc agatggtgca ggccgtggcc gaattcgtgg aacagcgtga tcacctcgtc
     1981 gtgggtgaac attgccggcc ggccaccgac cggggcggag aaattacagt tcagataggc
     2041 taccggggtc tggatgccgg cggccttgcg ccgccgggta atggcgtcgt ccatccacgc
     2101 tcctccgcgc ttggacggcc gcgcatacaa atcgagatag aactgcccga ccagcgcgcc
     2161 gtccgcattg cggatggaga agaagcgcac agcctcatgc cagcgcggtg catcatcggc
     2221 ggcgataccc agcccgtaaa gcgtctcgac cacacggaac atgccggcga gaacctgctg
     2281 ctcagggaag tattgtttga cttcctgatc cgagaacgca tagcgcgcca cccgcaactt
     2341 ttccgatccg taggctacgt cccagggctg caattcggcg agccccagtt gcctcgccgc
     2401 aaactcctcc agttcggcca gatcgcgctc ggcgtagggc ttggcgcggg tcgccagctc
     2461 gtcgagaaag cgcagcacct gcgcggggga gtcggccatc ttgggctgca aggacaattc
     2521 ggcaaaagtc ttgaagccga gcagttgcgc ctgttcgcgc cgtaacttca gaatttcgct
     2581 gatgagcgcg gagttgtccc acgccggatt gccgaactcg gaggcgcggg tggcataggc
     2641 gtgatacatc cgctcgcgca attcgcggtt gtccgcgtac tgcatgaccg gaagatagga
     2701 cggcatctgc agcgtgagtt tccagcccgt cttgccgatt tccgtcgcgg ccttgcgtgc
     2761 cgcttccagc acgtcgtcgg gaatgccgga caattgcgcg cggtcctgga tgatcaactc
     2821 gaaggcattg gtggcatcga gcaggttgtc gttgaattcg gatgacagct tggcgagccg
     2881 ctcggtcacc tgcttgaacc gcgccttgcg ttcggcggga agttccgcgc cgcccaggcg
     2941 aaaatcgcgt agttcattgt cgatgacttt tctctgcgcc tgcgtgagcg cggaaaaacc
     3001 gggcgaggca cgcaacgcct tgtatttccg gaacagcgcc tcgctttgcg acaactcggt
     3061 gaagtactgg gtgatgcgcc ccaggttggc gttgtaggcc tcgcgcaatg ccgggctgtt
     3121 catcaccgcg ttcatgtgcg caacctgccc ccaggcacgg cgcagctgct cgttggcgtc
     3181 gtccaggggg gcgacgaaat tctcccagga cggttcccgg cgatcggcct cctgccgggc
     3241 gatcagttca cggttcgccg ccagcaactg gtccagcgcc ggggtgacat gctccggctt
     3301 gaagtcggca aaacgcggca gacccgaaaa atcgagcaaa gggttcatgg agtcagtgtt
     3361 gaattttgag ttttgagtgt tgagtgttga gtgtttagtt ggtgtcgcga ccgccaaatt
     3421 caagccacat caaacttaac actcaacatt caacactcgt acacaacgtg gccgccgacc
     3481 agcgtatagc gcacccggcc attgacttca tagccggcaa agggcgtgtt cttgccctgg
     3541 ctcttgagcc tggccggttc gaccttccat cgcaaatgcg gatcgaagat gcacagatcg
     3601 gcgttggccc cggctgcgat cgcaacggcg ccgatgccga ggatgcgcgc cggttcggta
     3661 gtcactttcg cgatcgcctt cgggagcgca atgcgtgatt cctccgacca cttcagcgtc
     3721 agcgccagca gcagttccag cccggtggcc ccggcctcgg attcgccgaa cggcaattgc
     3781 ttggcgtcct catccaccgg ggtgtggtcg gaacagatcg catcgatggt gccgtcctcc
     3841 acgccacggc gcaacgcatc gcggtcgcgc aggcttcgca gcggtggtac caggtggtaa
     3901 ttggaatcga agtaaccgat atccatctcg ctcaggtgca catgattcgc cgatacgtcg
     3961 caagtcacgt tcatgcccag cgccttggcc tgacgcatca tctccacgcc ctccgcgctc
     4021 gacagccggc acagatgcag ccgcgcgccg gtttcgcggg caagcagcag catcgtggac
     4081 agcgccaccg tttccgccgc caccgggatc ggcgcgagcc cgaggcgcgt agccacctgc
     4141 ccgtcatggg cgacgccgcc gcgcgccagg ctgccgtcgc tcgggcgcag ccacaccgag
     4201 tagccgaagg tggtcgcata ctgcatcgcc cgcagcatca cctggttgtc gtcaagcggc
     4261 acttccgcct gcgaaaaagc cacacatccg gcatcgacca gctcggccat ttcggtaatg
     4321 cgctcaccct tcagccccag ggtcagtgcg cccagcgggt acacgtgagc ctggttgaga
     4381 ctgcgcgccc gatgcttgag catttccacc agacccggtt cgtccagcgg cgggtcggta
     4441 tccggaggac acgccagcga agtcactcca ccggccacag cagccgccat ctcactttcc
     4501 agcgtggcct tgtgctcgaa acccggctcg cgcaaccgcg ccgagagatc gaccaggccc
     4561 ggagacacca ccagtccgtt ggcctcgatc acccggttcg catggaatcc gtcgggcgcc
     4621 cggccgatgg ccgcaatctt tccagcggcc agatagaggt cctgaacctt ctcggtcccg
     4681 ctctgcggat ctaccactcg gccgttcttg atgtgaatct tcattcctta agctctaacc
     4741 gcagaggaca ccgaggacgc ggaggaaaac caaaaacgag gaaaaacgaa ttggagatag
     4801 atgcgaggtg gctgttcgaa atccgctgcc gccaatttcg actttgccgg aacatccgcc
     4861 aattggagct ttttacactc attttgcttt ttcgctcctg cctttcctct gcgtcctccg
     4921 cgtcctccgc gatgagcgct attacgcatt gccggccagg atcgacatca ccgccatgcg
     4981 cacggcgatg ccaaaagtca cttgcggcag gatcaccgac tggttgccgt cggccacgct
     5041 ggaatcgatc tcgacaccgc ggttcatcgg cccgggatgc atcacgatcg cgtccggctt
     5101 cgcgccggct agtttctccg gcgtgagtcc gtagtactt
//
LOCUS       DASQOP010000358        20657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_992802, whole genome shotgun sequence.
ACCESSION   DASQOP010000358 DASQOP010000000
VERSION     DASQOP010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 20657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20657
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992802"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..706)
                     /locus_tag="VE030_13840"
     CDS             complement(158..706)
                     /locus_tag="VE030_13840"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HZF24536.1"
                     /translation="MRWLSTMLALWYGAALATTEGIHVKSAELSLNGDQYYLEANFEV
                     GLTPTLEDALNKGLPLHFVIEFDLIHPRWYTAYLWNKSMAELQQRYRLSYNALTRQYR
                     LSFGALHQNFDALDDALALLGRLRRRFVVDADALDEGKVYEAAIRMRLDVSQLPKPFQ
                     INALASRDWNLSSEWFRWTLKR"
     gene            complement(711..1976)
                     /gene="rsmB"
                     /locus_tag="VE030_13845"
     CDS             complement(711..1976)
                     /gene="rsmB"
                     /locus_tag="VE030_13845"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HZF24537.1"
                     /translation="MRNIQHIAAQSVAAVLSGRNLNHTLEAAWKRAHALTRADRGAIQ
                     DLCYGTLRHLGRLRAVLLQLVPRPVEKPELQSLLLVALYQLEYSGAAPYAVVDHAVEC
                     AAKIGGRHVKAFVNAVLRNFQRGRAALLKEADSTDSGYYSYPAWWIERLRRDFPSEAQ
                     NILQTGNTHPPMTLRVNRRRGTPQEYLQELERAGLGARILDGGALNLLRPVPVEQLPG
                     FGQGMVSVQDAGAQFAARLLDLSDGMRVLDACAAPGGKTAHMLELGDVDMTALDADPA
                     RLKRVSSNLERLGLSARVSCADAAAIDAWWDNRPFDRVLLDAPCTASGVVRRHPDIKW
                     LRRPGDVRRFAEQQQRLLEALWKVLGRGGKLLYVTCSIFMEENQERIGGFAARHPDAR
                     VLGAMPGRDGLLLPDNDHDGFYYALLQKD"
     gene            complement(1990..2919)
                     /gene="fmt"
                     /locus_tag="VE030_13850"
     CDS             complement(1990..2919)
                     /gene="fmt"
                     /locus_tag="VE030_13850"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019717338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HZF24538.1"
                     /translation="MRIVFAGTPAFAASALQALIGAGHEIALVLTRPDRPAGRGLRLA
                     ASEVKQLADHARLPLLQPQTLKDAQLQSRLHAAGAQSMVVAAYGLILPRAVLDLFPAG
                     CINIHASLLPRWRGAAPIQRAILGGDSETGISIMRMEEGLDSGPVYLMRPIPIAAEDT
                     AGTVHDKLAALGARCIVEALPAIAEGTLEAVPQSEAGATYAPKITKDEAAIDWRREAV
                     EVDRQVRAFNPFPGAYSTLHDGPLKVWRAAVVPGSPAPPGTILRADAQGIVVACGNGA
                     LRATELQRAGGKRLAATDFLRGLPLAAGERFGA"
     gene            complement(2919..3422)
                     /gene="def"
                     /locus_tag="VE030_13855"
     CDS             complement(2919..3422)
                     /gene="def"
                     /locus_tag="VE030_13855"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HZF24539.1"
                     /translation="MAILPILRYPDPRLRKVAKPVAVVDDEIRKLARNMAETMYAAPG
                     VGLAATQVDVHKQLILIDLSETRDELLVLINPHILESRGKSDCEEGCLSLPGIFEKIA
                     RAEWVKVRALDLDGKSFTTQATGLLSVCIQHEMDHLKGKIFIEYLSRLKQTRILSKLK
                     KQQRQTL"
     gene            3546..4610
                     /locus_tag="VE030_13860"
     CDS             3546..4610
                     /locus_tag="VE030_13860"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZF24540.1"
                     /translation="MGKSIISLLLLLTAWMAPGTSVADELALAENAPDRYVVVKGDTL
                     WDISARFLRDPWRWPDIWGLNKDQIKNPHWIYPGDVILLDFTGMTPRLRLQGIDGATD
                     WRLVVTKLSPAIRKLDLAASAIPSIPLEAIAPFLTKPLVVGEGEMSGAPILVAGQENR
                     VILSAGDTAYAKGVVSEDGTEWNIYRPGRNLVDPDTKELLGREAVHLGELKVSQFGDV
                     SRVAITKSVLEITPGDRLVKIVPVPVLPYIPHAPSKQIKGKVIAASHDSVTEIGPHMV
                     VVLNRGAREGLETGNVLALYRDLPMVKPAGATNPKERIKLPAERYGVVFVFRVFEKVS
                     YALVMHTTRPVNVLDAVQTP"
     gene            4628..5713
                     /gene="dprA"
                     /locus_tag="VE030_13865"
     CDS             4628..5713
                     /gene="dprA"
                     /locus_tag="VE030_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HZF24541.1"
                     /translation="MPPDQELLAWLKLGLVAELTPASFRKLLGEFGDPEAVCAANRSA
                     LCRIVANEVADAILRGPDTTCVDLALRWLEEPGNRVISLADAAYPRLLLEITDPPPLL
                     YVKGNTELLNCAALAVVGSRNASPQGSANAEAFARELSDGGFTVISGLALGIDAAAHR
                     GGLAGASSSLAVVGTGLDIVYPARNRDLAHQLAVQGALVSEFSLGTPALSGNFPRRNR
                     LISGLARGCLIVEAALRSGSLITARYALEQGREVFAIPGSIHSPLSKGCHLLIKQGAK
                     LVESSNDILEELGAAVRVESATPGKADASPEHVELLAALGFDPLDLDTLCERSGLTPE
                     TASAMLLTLELEGTVSRLPGGRFQRVR"
     gene            5748..6200
                     /locus_tag="VE030_13870"
     CDS             5748..6200
                     /locus_tag="VE030_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HZF24542.1"
                     /translation="MFDILVFLFENYFHAGAYPDQQTLTKRLSAAGFDSRDISQALTW
                     LSGLERVGHSNSRFSSSDGFRFYTEQELAGLTAEARGFLLFLETAGVIKALQRELIIE
                     RVLALDEPAVDLEKVKLIVLMVLWNQNESVDSLVLEELVAPNQERYLQ"
     gene            6282..8762
                     /gene="topA"
                     /locus_tag="VE030_13875"
     CDS             6282..8762
                     /gene="topA"
                     /locus_tag="VE030_13875"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HZF24543.1"
                     /translation="MSQNLLIVESPSKAKTLKKYLGRGFDIVASYGHVRDLEAKEGAV
                     DPADNFHMKYALVEKNRKHVDAIAKAAAKANRIYLATDPDREGEAIAWHVGEILKGKK
                     LLKNKKLHRVVFYEITESAVTDAVAKPRQISMPLVNAQQARRALDHLVGFKLSPLLWK
                     KIGPGLSAGRVQSPALRLIVERELEIEKFKSREYWTIHLDSHKEKQKFGARLFQYRGD
                     KVEQFTIVTDEQHRQVVADIEKAAKGTAKVVRVERKPKSRQPAPPFTTSTLQQEAVRK
                     LGFTTERAMRTAQQLYEGVDIGGSTVGLITYMRTDSQSLANEALQELRDYIQKKFNTD
                     YLPRAPIVYRSKSKNVQEAHEAIRPTSVFRTPESIRKYLTSEQAALYEMIWKRALACQ
                     MTPARYDTTTVDLAVGRADTLFRATGQILVFPGFMAVYLEDADDAEQEEEARLPRLEE
                     KETVPIDRLFGEQHFTQPPPRYTEASLVKTLEEYGIGRPSTYASIISTLQDRGYALLE
                     KKRFQPTDIGRLVNGFLTSHFGHYVDYGFTANLEEELDEISNGERDWIPVLKDFWKDF
                     ARDLKSKEKVARGSPLEESCPKCGKQLFLQNSRRGMFIGCSGYPECDYTKPWGNQPEG
                     PVILGSDPATGLEVLLLKGPYGFYAQLGQTPQDKSVKPKRAAWPKNLPAASADLDTAL
                     KLLALPRQLGLHPETGKPIEASIGRFGPYLKHDGMFKSIPKADNVHDIALARAIQLLA
                     EKKAGGMSGRALGAHPEDGRSVVVHSGRYGPYVKHGKINATIPPSADPAKLTLQEAVE
                     LLAAKSAKGSTGKKKPPAKPKGPKRKAA"
     gene            complement(8772..11168)
                     /gene="gyrB"
                     /locus_tag="VE030_13880"
     CDS             complement(8772..11168)
                     /gene="gyrB"
                     /locus_tag="VE030_13880"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HZF24544.1"
                     /translation="MSESEYNSDSIKVLKGLEAVRKRPGMYIGDTSDGTGLHHMVFEV
                     LDNSIDEALAGHCDEIKVVIHGDNSISVVDNGRGIPTGIHPEDEEGRSTAEIVMTVLH
                     AGGKFDSNSYKISGGLHGVGVSVVNALSDWLKLTIRRDGQVNFMEFRNGDPVSPLKVV
                     GQTERRGTEVHFHPNPEVFGNIEFHYDILAKRLRELSFLNNGVRIELHDQRNAKEETF
                     AFAGGVKGFVEYINRSKTVLHPNIFHAICERDGIGVEVAMQWNDSYQESVLCFTNNIP
                     QRDGGTHLTALRAAMTRTLNQYIEANEQAKRAKVETTGDDMREGLSCILSIKLPDPKF
                     SSQTKDKLVSSEVRPVVEDAISQALHDWLLEKPSDAKIIVSKIIEAARAREAARKARE
                     MTRRKGVLDGFGLPGKLADCQEKDPALCELYLVEGDSAGGSAKQGRDRKFQAILPLKG
                     KILNVERARFDKLVSSQEIATLITALGTGIGKEDFKPEKLRYHRIIIMTDADVDGSHI
                     RTLLLTFFYRQMPDLVERGHIYIAQPPLYKVKHGKEERYLKDDHELKEYLLRLALKEA
                     ELYPGGGGPALAQDALESVARSYILAEAVIERLGKRIDPAVLAALLKDPEISLADAQA
                     ARRSAERLTALCKPAGLAIEPQYDQKNETHVLVIKRLRHGNLRIGYLDNEFLDSGDYA
                     QIRRTAQVLHGLLAEGAYVKRGDQERRVGEFKEALDWLLEEVKKGVSIQRYKGLGEMN
                     PEQLWETTMDPKSRRLLKTQIEDAITADEIFTTLMGDMVEPRRAFIETNALGVRNLDV
                     "
     gene            complement(11191..12297)
                     /gene="dnaN"
                     /locus_tag="VE030_13885"
     CDS             complement(11191..12297)
                     /gene="dnaN"
                     /locus_tag="VE030_13885"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HZF24545.1"
                     /translation="MKILEIEKDVLLRPLQAVSGIVERRHTLPILSNVMLEADGTALN
                     LLATDLEIQVATSAILEKAASRYAVTVSARKLQDILRSLPDKTTVSLDAQEGRLQVKA
                     GKSRFNLQTLPAQDFPKLADGGNGAVSVRLSQKVLRQQLLLVQYGMAQQDIRYYLNGL
                     LLMVEDSQLTMVATDGHRLAYAAAKLPQPVARSEVIVPRKAVLELAKLLADGEDEVKI
                     DILPNQVQFRFSGIDLVSKVVDGKFPDYTRVIPTNYKKHFILDRLLLQQALQRAAILS
                     NEKFRGVRWVIGASSLKIVCTNAEQEEAEEELDIDYNGEALDIGFNITYLLDVLNNLD
                     TKDVDCAVGDANSSMLMSVPGNKEFKYVVMPMRI"
     gene            complement(12473..13798)
                     /gene="dnaA"
                     /locus_tag="VE030_13890"
     CDS             complement(12473..13798)
                     /gene="dnaA"
                     /locus_tag="VE030_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HZF24546.1"
                     /translation="MDSLWNHCLRHFEKSLPAQQYKTWIQPLHAHLDQHTVVVSAPNR
                     FVLEWVKDKFVSEIERLSSDFLRKPTHVVLTLSENAAEETVQPVPEAEAPAPKPKIRE
                     QSRLSPSFTFDSFVTGKANQLARAAALQVSEHPGVAYNPLFVYGGVGLGKTHLIQAIG
                     NLLLELSPSAKVRYIHAERYVSDVVRAYQHKAFDGFKKYYHSLDLLLIDDIQFFSGKN
                     RTQEEFFYAFNALIEAHKQVIITCDTYPKEIAGIEDRLISRFGWGLTVAIEPPELEMR
                     VAILLKKAEAERVSLPEDIAFFVATHIQSNVRELEGGLKRVLAYAKFANTRLSVDLAR
                     EALKDLLAVQVRQVSVENIQRIVADYYKIKVSEMYSKKRSRMVARPRQVAMALAKELT
                     HLSLPDIGDAFGGRDHTTVLHACRKIADLRTSNADITRDFDALLQTLRG"
     gene            13984..14118
                     /gene="rpmH"
                     /locus_tag="VE030_13895"
     CDS             13984..14118
                     /gene="rpmH"
                     /locus_tag="VE030_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HZF24547.1"
                     /translation="MKRTYQPSKTRRSRTHGFLVRMKTRGGRAVINARRAKGRARLAV
                     "
     gene            14115..14459
                     /gene="rnpA"
                     /locus_tag="VE030_13900"
     CDS             14115..14459
                     /gene="rnpA"
                     /locus_tag="VE030_13900"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HZF24548.1"
                     /translation="MTQPRPKLPRLARLCGGAHFTGAFSARRQGRFFIVLTRNNPGAK
                     SARLGIVIGRRAAPKAVTRSAMKRLVREVFRHLRSDLGSTDFVVRVRRPAVREEMFEA
                     RGELQSLLSGRR"
     gene            14459..14668
                     /gene="yidD"
                     /locus_tag="VE030_13905"
     CDS             14459..14668
                     /gene="yidD"
                     /locus_tag="VE030_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HZF24549.1"
                     /translation="MRATLVNLIKLYQYLLSPWVGNHCRFYPTCSEYAKAAIETHGVL
                     RGSWFAGKRLLRCHPWQPGGADPVP"
     gene            14685..16340
                     /gene="yidC"
                     /locus_tag="VE030_13910"
     CDS             14685..16340
                     /gene="yidC"
                     /locus_tag="VE030_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020693854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HZF24550.1"
                     /translation="MDSLRLFAAMIFAVSVFLLFDAWVKERNPPPPPQDAAQQAVTAT
                     PSPTLPPGVTTPASPAAPPAKAAASSAANLPVITVHTDVLNAEIDPVGGVLRRLEFTQ
                     HKDKVDKNRNFILFEQSADHTYVAQSGLIGDKLPNHTTPYRAANANYQLSDGNDEVTV
                     TLTAPESDRIAVTKRITFQRGSYLIHESFRIVNGGSDELKPFAYFQFLRDGKPAAGDS
                     KVVPTYTGAAVYTDQSKFRKVTFSDIDKGKTDYPKHVDDGWIAFVQHYFVAAWLPAGE
                     TPREFYTRALSNGLYAAGVIVPVGGIAPGASGQVDLRLYAGPQEQEKLAKLAAGLELT
                     VDYGWLTVIAAPLFWVLSAIQKWVNNWGVAIIILTIIIKLIFYPLSAASYKSMAKMRV
                     LGPKMQKLKEQYGDDRQRMQQAMIEMYKTEKINPLGGCLPIVVQIPVFIALYWVLLAS
                     VELRNAPFMLWITDLSAQDPYYILPVLMGITMIIQTSLNPTPPDPVQAKVMKIMPVVF
                     SVFFFFFPAGLVLYWLVNNILSIAQQWQITRALERSKPAHVPR"
     gene            16327..17670
                     /gene="mnmE"
                     /locus_tag="VE030_13915"
     CDS             16327..17670
                     /gene="mnmE"
                     /locus_tag="VE030_13915"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HZF24551.1"
                     /translation="MSRADTIAAIATAPGRAGIGVVRVSGSGVRAIIAGILGRVPQPR
                     TATLHNFHDAAGGTLDRGLALFFAAPHSYTGEDVLELHGHGGAAVLQLVLRRCLDLGA
                     RPAQPGEFTRRAFLNDKLDLAQAESVADLIDASSEAAARGAMRSLAGEFSARIDHLVS
                     ALIELRALVEATLDFPDEESDFLERGDAFKRLARLRAELGEVQRAAEQGRILREGARC
                     VLVGQPNVGKSSLLNRLAGDEVAIVTEIPGTTRDPLRHELVIDGVPVHVIDTAGLRES
                     QEPIEKIGIERAWREIGQADLAMLVVDVTRGMANADRAILAKLQEKVKKIFIFNKIDL
                     SGDPPSLCELDDRVEIRVSAKSGAGLELLRANILRAVGWRPVEEMQFMARERHLRALQ
                     QADAALARAAAVRAKAIELFAEELRLAQQALGEITGQFSADDLLGEIFSRFCIGK"
     gene            17677..18441
                     /locus_tag="VE030_13920"
     CDS             17677..18441
                     /locus_tag="VE030_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HZF24552.1"
                     /translation="MIDVLVLCVFAFFGGLVDAVAGGGGLLLVPALFAVYPSAPPALL
                     LGTNKLSAIAGTTFATLRYAWSVPIRWRTILPAALVACGGGLAGAKAVTLIEPTVLRP
                     LILVMLGAVAIYTLVRQNFGSAPDGRVRLKIEGPGVAVLAAVIGFYDGFFGPGAGSFF
                     LFGLVRLFGYDFLAAAAATKVLNLATNFGALMLFVASDNVLYAVGLPMAASSIAGGLL
                     GAQLAVRQGSRFIRWMFLLVVAALIVKLAYDLLLRP"
     gene            18579..20513
                     /gene="mnmG"
                     /locus_tag="VE030_13925"
     CDS             18579..20513
                     /gene="mnmG"
                     /locus_tag="VE030_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010811274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HZF24553.1"
                     /translation="MQHSNSFDIIVVGGGHAGTEAALAAARAGCCTLLLTHNIETLGQ
                     MSCNPSIGGIGKGHLVKEVDALGGAMAAATDEAGIQFRILNSSKGPAVRATRAQADRV
                     LYRQAIRRRLENQKNLTIFQQAVDDLSLDGERVAGVVTQLGLRFYARTVVLTTGTFLS
                     GLVHVGLDHYQAGRAGDPPAISLARRLRELQLPVGRLKTGTPPRLDGRSIDYSVMTEQ
                     PGDDPVPVFSFLGSAGQHPRQLPCWITHTNLRTHEIIRGGLDRSPMFTGIIEGVGPRY
                     CPSIEDKVTRFAEKNSHQIFLEPEGLTTNEVYPNGISTSLPFDVQAELVHSIKGLENA
                     HLTRPGYAIEYDYFDPRGLKSSLETKAIKGLFFAGQINGTTGYEEAAAQGLLAGINAA
                     LLVQGKEPWCPRRDQAYLGVLVDDLITRGVTEPYRMFTSRAEYRLSLREDNADMRLTE
                     IGRRLGVVDDARWEFFEHKREVVARELERLKTTWVNPKQLAEETMLQLFGQPLDRDYT
                     LEELLRRPSVCYRDLMALTAAGPGVTEDQAAEQVEVQTKYRGYIERQREEVERQEHFE
                     SLCLPANLDYRSVRGLSIEVQQKLNKHKPETLGQAARISGITPAAISLLLVHLKRGIK
                     ATAEAGARHGSQVRAAGNRG"
BASE COUNT         3965 a         6296 c         6302 g         4094 t
ORIGIN      
        1 cccagcagca gcggcagatc gccggagaac agcggcgtgt cggcgctgac gctggaaagc
       61 aggtacagca tgaccgcgcc gagcaacagt accagcgtga ttagtagctt cataccgcgg
      121 ctcatcagcg aacgcctgag ggcgcgtgta atggcagtca tcgcttcaga gtccagcgaa
      181 accactcgga tgaaaggttc cagtcgcgcg aagccagcgc attgatctgg aacggcttgg
      241 gtaattgcga gacgtcgagc cgcatgcgaa ttgcggcctc gtataccttg ccctcgtcca
      301 gcgcatcggc atcgacgacg aaacgccgac gcagtcgccc gagcagggcc agcgcgtcgt
      361 ccagcgcgtc gaaattctga tgcagggcac cgaagctcag ccggtactgg cgcgtgagcg
      421 cgttgtaaga caaccggtag cgttgctgta gttccgccat cgatttgttc cacaggtaag
      481 ccgtgtacca gcgcggatgg atgagatcga attcgatgac gaaatgcagc ggcaagccct
      541 tgttgagcgc atcttcgagt gttggagtga gaccaacttc gaagttggct tctagataat
      601 attggtcacc gttgagtgag agttccgccg acttgacgtg gatcccctcg gtcgtcgcca
      661 gcgccgcgcc gtaccacagc gcaagcatgg tggacaacca ccgcaacgtg ctagtccttc
      721 tgcaggaggg cgtagtaaaa accatcgtga tcgttgtccg ggagcagcag tccatcgcgc
      781 cccggcatcg cacccagaac acgcgcgtcc gggtgccgcg ccgcgaaacc gccgatcctc
      841 tcctggttct cctccatgaa aatcgagcag gtcacataga gcaatttacc accgcggccg
      901 aggaccttcc acagcgcttc gagcaagcgc tgctgctgtt ccgcgaagcg ccgcacgtcg
      961 ccgggccggc gcagccattt gatgtcggga tgacggcgca ccacgcccga tgcggtgcag
     1021 ggcgcatcga gtaacacccg gtcgaacggc ctgttatccc accaggcatc gatcgccgcg
     1081 gcatccgcac agctgacccg cgcactcagg ccgaggcgct ccaggttcga gctcacgcgc
     1141 ttgaggcggg ccggatcggc atcgagcgca gtcatatcca cgtcgccgag ctcgagcatg
     1201 tgcgcggtct tgccgccggg cgcggcgcag gcatcgagca cgcgcatgcc atcgctcaga
     1261 tcgagcagtc gcgcagcaaa ctgtgctccg gcatcctgca cagacaccat gccctggccg
     1321 aaccccggca gttgctcgac gggtaccggg cgcagcaaat tgagcgcgcc gccatcgagt
     1381 atgcgcgcgc ccagccccgc ccgctccagt tcctgcaggt attcctgcgg cgtaccgcgc
     1441 cgccggttga ccctcagcgt catcggcgga tgcgtgttgc cggtctggag aatattctgt
     1501 gcttcggacg ggaagtcgcg ccgcagccgc tcgatccacc atgcgggata agagtagtag
     1561 cctgagtcgg tgctgtctgc ttcctttaac agcgcggcgc ggccgcgctg gaaattgcgt
     1621 agtacggcgt tgacgaacgc ctttacgtgg cgaccgccga ttttcgccgc gcattccacc
     1681 gcgtgatcca ctacggcgta gggcgcggcc ccgctgtatt ccagctggta aagcgcgacc
     1741 agcaatagcg attgcagctc cggcttctcc accggcctgg gcacaagctg caacaacacc
     1801 gcgcgcaatc tgcccagatg tcgcaacgtc ccgtaacaga gatcctgtat cgctccccgg
     1861 tcggcgcgcg tgagcgcgtg cgcccgtttc caggccgctt ccagcgtgtg attcaggttt
     1921 cttccggaaa gcaccgcagc cacggattgc gctgcaatat gttggatatt gcgcaccgcg
     1981 gtccgggact caggcgccga agcgttcgcc ggcggcgagc ggcagtccgc gcaggaaatc
     2041 cgtggccgca aggcgcttgc cgcccgcacg ctgcaactcg gtggcacgca atgcaccgtt
     2101 gccgcaggcg acgacaatac cttgcgcatc ggcacgaagg atggtcccgg gcggtgcggg
     2161 cgaacccggc acgaccgcag cgcgccagac cttcaacggt ccgtcgtgca gcgtggaata
     2221 ggcgccgggg aagggattga acgcgcgcac ttggcgatcc acttcgaccg cctcgcggcg
     2281 ccagtcgatc gccgcctcgt ccttggtgat ctttggggcg taggtcgcgc cggcttcgct
     2341 ttgtggcacg gcctcgagcg tcccctcggc gattgccggg agcgcttcga cgatgcaacg
     2401 cgcgcccagc gccgcaagct tgtcatgtac ggttcccgcg gtgtcctcag cggcgatcgg
     2461 gatggggcgc atcagataga ccggaccgct gtccagtcct tcctccatgc gcatgatcga
     2521 aataccggtc tcgctgtcgc cccctaggat ggcgcgctgg atcggcgcgg caccgcgcca
     2581 gcgtggcagc agcgaggcgt ggatattgat gcagcctgcc gggaacaggt cgagcaccgc
     2641 acgcggaagg atcagcccgt aggcggcgac caccatggac tgcgcgccgg ctgcgtgcag
     2701 gcgcgactgc agttgcgcat ccttgagcgt ttgcggctgc aacagcggaa gccgggcgtg
     2761 gtcggcgagt tgcttgactt cgctcgcggc gagcctgaga ccgcggccgg cgggccggtc
     2821 cggccgggtc agcaccagcg cgatctcatg cccggcccca atcagcgcct gcaacgcact
     2881 ggcggcgaac gccggcgtgc cggcaaatac gatccgcatc agagcgtctg ccgctgctgt
     2941 ttcttcagct tgctcaggat gcgggtctgc ttgaggcgcg acaggtattc gatgaagatc
     3001 ttgcctttga gatgatccat ctcatgctgt atgcacaccg acaacagtcc ggtggcttgg
     3061 gtcgtgaacg acttgccgtc cagatcaagc gcgcgaacct tcacccactc cgcgcgcgcg
     3121 atcttctcga agattccggg cagcgacagg cagccctctt cgcaatcgga cttacctcgc
     3181 gattcgagaa tgtgcgggtt gatcagcacc agtaactcgt cgcgggtttc ggagaggtcg
     3241 atcaggatga gctgcttgtg cacgtccacc tgggtggccg ccaggcccac acccggggcc
     3301 gcatacatgg tctcggccat gttcctcgcc aacttgcgga tttcgtcgtc gactactgcc
     3361 accggcttcg ccactttgcg cagccgcgga tcggggtagc gaaggattgg gagtatcgcc
     3421 ataattgctt gctaatttaa taaactagat gcagaattta aaccaaagta tcgagtctgg
     3481 cgccaggcgg gactgcgtac ccggcgaggg atgcgattct ggatcgacag aaatgggggc
     3541 ctgagatggg caaatcaatt atatccctgc tgctgctgct gaccgcgtgg atggcgccgg
     3601 gaaccagcgt ggcggacgag ctcgcgctgg ccgaaaatgc gcccgatcgc tatgtcgtcg
     3661 tgaagggcga tacgctttgg gacatctcgg cgcgctttct gcgcgatccg tggcgctggc
     3721 ctgatatctg gggcctgaac aaggaccaga tcaagaatcc ccactggatc taccccggcg
     3781 acgtgatcct gctcgacttc accggcatga ccccacggct gcgcctgcag ggcatcgacg
     3841 gcgccaccga ctggcgtctg gtggtcacca agctcagtcc ggcgatacgc aaactggatc
     3901 tcgcggcaag cgcgatcccc agcattccct tggaggccat cgcgcctttc ctgaccaaac
     3961 cgcttgtggt cggcgagggc gaaatgtctg gcgcgccgat cctggttgcc ggacaggaaa
     4021 accgggtcat actctccgcc ggcgataccg cctacgccaa aggcgtggtc tctgaggatg
     4081 gcacggagtg gaacatatat cgcccgggcc gcaatctggt cgatccggac accaaggaat
     4141 tgctcgggcg cgaggccgtg cacctgggcg aactgaaagt aagccagttc ggcgacgtca
     4201 gcagggttgc gatcaccaaa tcggtcctgg aaattacccc cggcgaccgc ttggtaaaga
     4261 tcgtgcccgt tccggtgcta ccctacatcc cgcacgcgcc gagcaagcag ataaaaggca
     4321 aggtaatcgc agcctcgcac gacagcgtca cggaaatcgg tccgcatatg gtggtggtat
     4381 tgaaccgcgg cgcacgcgaa ggtctggaaa caggcaacgt gttggccctg tatcgcgatt
     4441 tgcccatggt caagccggcc ggtgcgacca atccgaaaga gaggatcaag ctgccggctg
     4501 agcgctacgg tgtcgtgttc gtgttccggg ttttcgagaa agtatcctac gcgctggtga
     4561 tgcataccac gcggccggtc aacgtgctcg acgcagtgca aacgccctag cctggcgccg
     4621 acggcgcatg ccgccggatc aggaactcct cgcctggctc aagctcggcc tcgtcgccga
     4681 gcttaccccc gcttctttcc gcaagctgct cggcgaattc ggcgacccgg aagccgtatg
     4741 cgcggcgaac cggtctgcgc tctgccgcat cgtcgcgaac gaagtcgcgg atgccatcct
     4801 gcgagggccg gacacaacct gcgtcgacct cgcgctgcgc tggctcgaag agccaggtaa
     4861 ccgcgtcatc agtctcgcgg atgctgccta tccgcggctc ttgctggaga tcaccgaccc
     4921 gccaccgcta ctgtatgtga agggcaatac agaactgctc aactgcgcgg cgctggcggt
     4981 tgtcggcagc cgcaatgcca gcccgcaagg cagcgccaat gcggaggcct tcgcccgcga
     5041 actctccgat ggcggtttca cggtcatcag cggacttgcg ctcggcatcg atgccgccgc
     5101 acatcgcggc ggacttgccg gcgcctcctc ttcgctggcg gtggtcggca ccggtctcga
     5161 catcgtatat ccggcgcgca atcgcgatct cgcacatcaa ctggcggtgc aaggcgcgct
     5221 ggtttccgag ttttcgctcg gaacacccgc gctgtcgggg aatttcccta ggcgcaaccg
     5281 gctgatcagc ggactggccc gcggctgcct gatcgtggaa gccgcgctca ggagcggatc
     5341 gctgatcacg gcgcgttatg cgctggaaca ggggcgcgag gtgtttgcga tccccggttc
     5401 gatccactcg ccgctgtcga agggttgtca tcttctgatc aagcagggcg ccaaactggt
     5461 cgaaagcagc aacgacatcc tcgaggaact cggcgccgcg gtgcgtgtcg agagcgcaac
     5521 gcccggcaag gcggatgcct cgcccgaaca cgtcgaactg ctcgcggcgc tcggtttcga
     5581 cccgctcgac ctcgacacat tgtgcgagcg cagcggcttg acgccagaaa ccgcttcggc
     5641 gatgctgttg acactggaac tcgaaggcac cgtcagccgg ctaccaggcg gcaggttcca
     5701 gcgcgtgcgc tagcgtcagg ggcgtcaata cctccagaca ggggctcatg tttgacatac
     5761 tcgtcttcct gttcgaaaac tactttcatg ccggtgccta ccccgatcaa caaacactta
     5821 ccaagcgcct gtccgctgcc ggattcgaca gcagggacat ttcacaggcc ctgacctggc
     5881 ttagcggcct ggagcgcgtc ggccacagta actcccgctt ttccagcagc gacggatttc
     5941 gcttttacac cgagcaggaa ctggccgggc tgacagccga ggcgcgcgga ttcctgctct
     6001 ttctcgagac cgcgggcgtg atcaaggcat tgcagcgcga gctcatcatc gagcgggtgc
     6061 tggcgctcga tgagcccgca gttgacctcg agaaagtaaa gctcattgtc ctcatggtgt
     6121 tgtggaacca gaacgaatcg gtggattctc tggtgctcga agaactggtc gcgccaaatc
     6181 aggaacgcta tctgcaataa tccgctgttt cctgaacaag cgcttgctcc gccgccggca
     6241 agttcttatc attccgccct tcgttttcgg ccggacgcgc aatgtctcaa aatctgctga
     6301 tcgtcgagtc tccctccaag gcgaagactc tcaaaaagta tctgggccgc ggcttcgata
     6361 tcgttgcctc ttatgggcac gtccgcgacc tggaagccaa ggaaggcgcg gtggacccgg
     6421 cggacaactt ccatatgaag tacgcgctgg tcgaaaagaa caggaagcat gtagacgcaa
     6481 tcgccaaggc ggcggccaag gccaacagga tttatctggc caccgacccg gaccgtgagg
     6541 gtgaggccat tgcctggcac gtcggcgaga tcctcaaagg caagaagctg ctcaagaaca
     6601 agaaacttca ccgcgtcgtg ttctatgaga tcacggaatc cgctgtgacc gatgcggtcg
     6661 ccaagccgcg ccagatttcc atgccgctgg tgaacgcgca gcaggcgcgc cgcgcgctcg
     6721 accatctggt aggcttcaag ctgtcgccgc tgttgtggaa gaagatcggc cccgggctgt
     6781 ccgccggccg ggtacagagc ccggcgctgc gactcatcgt cgaacgtgag ctcgaaatcg
     6841 agaaattcaa gagccgcgag tactggacca tacatctcga cagtcacaag gaaaaacaga
     6901 aattcggcgc gcgcctgttt cagtatcgcg gcgacaaggt cgagcaattc acgatcgtca
     6961 ccgacgagca gcaccggcaa gtggtcgccg acatcgagaa ggccgcaaag ggcaccgcca
     7021 aggtggtgcg cgtcgagcgc aagcccaaga gccgccagcc ggcgccaccg ttcaccactt
     7081 cgacgctgca gcaggaagcg gtgcgcaagc tgggctttac caccgagcgc gcgatgcgta
     7141 ccgcccagca actctacgaa ggggtggata tcggcggcag caccgtgggt ttgatcacct
     7201 atatgcggac cgattcacag tcgctcgcca acgaagcgct gcaagaactg cgcgactaca
     7261 tccagaaaaa attcaacacc gattatctgc cgcgcgcgcc gatcgtgtac cgcagtaaat
     7321 cgaagaacgt ccaggaagcg cacgaagcaa ttcgtccgac ttctgttttc cgcacgccgg
     7381 aatcgatccg caagtacctg acgtcagaac aggccgccct ctacgagatg atctggaagc
     7441 gcgcgctcgc ctgccagatg actccggcca ggtacgatac gacaacagtg gatctcgcag
     7501 ttggccgcgc agataccttg tttcgtgcca ccggccagat tctcgttttc cccggcttca
     7561 tggccgttta cctcgaagac gccgatgacg ccgagcagga agaggaggcc aggcttccgc
     7621 gactggaaga gaaggagact gttccgatag accggctctt cggcgaacag catttcaccc
     7681 agccaccgcc gcgttacacc gaagcaagcc tggtgaagac cctggaggaa tacgggatcg
     7741 gcaggccttc gacctacgcc agcatcattt ccacgctgca ggaccgcggc tacgcgctgc
     7801 tggagaagaa acgcttccag ccgaccgaca ttggccggct ggtgaacgga ttcctgacct
     7861 cgcacttcgg ccattacgta gactacggtt tcactgccaa tctggaagaa gaactggacg
     7921 agatctccaa cggcgagcgc gactggattc cagtgctcaa ggatttctgg aaagatttcg
     7981 cgcgcgacct caagtccaaa gagaaggtcg cccgcggcag cccgctcgag gaatcctgcc
     8041 cgaagtgcgg caagcagctc ttcctgcaga acagccggcg cggcatgttc atcggttgca
     8101 gcggctatcc cgaatgcgat tacaccaagc cctggggcaa ccagcccgag ggcccagtga
     8161 tcctcgggag cgatcctgca accggactcg aggtactgct gctcaagggg ccgtacggct
     8221 tttacgccca gctcggacag acgccgcagg acaagtccgt gaaaccgaaa cgcgcggcat
     8281 ggccgaagaa tcttcccgcc gcatcggcag acctggacac cgcgctgaaa ctgctcgcgc
     8341 tgccgcgcca gctcggtttg catccggaaa ccggcaagcc gatcgaagcc agcatcggac
     8401 gcttcggccc gtacctgaaa cacgacggca tgttcaagtc cattcccaag gccgacaacg
     8461 tgcacgacat tgccttggcg cgggcaatcc agctgctagc cgaaaagaag gccggaggta
     8521 tgagtggacg cgcgctcggc gcgcacccgg aagacggcag atcggtagtg gttcacagcg
     8581 gccgctacgg gccttatgta aagcacggca agatcaacgc caccattccg ccttcggcgg
     8641 atccggccaa gctgactcta caagaagcgg tggaactgct ggccgcaaag tccgcgaagg
     8701 gttccaccgg caagaaaaaa ccccccgcca agccaaaggg gccgaaacgc aaggccgcct
     8761 gaaggtggca gtcagacgtc gaggttgcgc acgcccaggg cgttggtttc aataaacgcg
     8821 cgacggggct ccaccatgtc ccccatcagc gtggtgaaaa tctcgtctgc cgtgatcgca
     8881 tcttcgatct gggtcttgag caagcgacgc gatttcggat ccatggtggt ttcccacaat
     8941 tgttccgggt tcatctcgcc gagtcccttg tagcgctgga tcgacacccc tttcttgacc
     9001 tcctcgagca gccagtcgag agcctccttg aactcgccaa cccggcgttc ctggtcgccg
     9061 cgcttgacgt atgcgccctc cgccaggaga ccgtggagca cttgcgcggt ccgtctgatc
     9121 tgcgcgtaat cgccgctgtc gagaaactcg ttgtccagat agccgatccg cagattgccg
     9181 tgccttaggc gcttgatcac cagcacgtgg gtctcgttct tctggtcata ttgcggctcg
     9241 attgccaggc cggccggctt gcacagcgcg gtgaggcgtt ctgcggaacg ccgtgccgcc
     9301 tgcgcgtcgg caagactgat ttcgggatcc ttcagcagtg ccgccaacac cgcaggatcg
     9361 atgcgcttgc cgagcctctc gatcaccgcc tccgcgagga tgtatgaacg ggccaccgac
     9421 tccagcgcat cctgcgccag cgccggccct ccgccgccgg ggtagagctc ggcttccttc
     9481 aacgccagcc gcagcagata ttccttcagt tcgtgatcgt ccttgagata ccgctcttcc
     9541 ttgccgtgct tgaccttgta gagcggcggc tgggcgatgt agatgtggcc gcgttcgacc
     9601 aggtccggca tctgccggta aaagaacgtc agcaacagcg tgcgtatgtg cgagccgtcg
     9661 acatccgcgt cggtcatgat gatgatgcgg tgatagcgaa gtttttccgg cttgaagtct
     9721 tccttgccga tgccggttcc gagcgcagtg atcagcgtgg cgatttcctg cgaagacacc
     9781 aacttgtcga aacgcgccct ctcgacattg agaatcttgc ccttgagcgg caggatagcc
     9841 tggaatttcc ggtcgcgccc ctgctttgcc gagccgccgg cggaatcacc ctcgaccagg
     9901 tagagctcgc acaacgccgg atccttctcc tggcagtcgg cgagcttgcc cggcaggccg
     9961 aatccgtcga gcacaccttt gcggcgggtc atttcccgcg ccttgcgagc ggcctcgcgc
    10021 gcgcgcgccg cttcgatgat cttgctgacg atgatcttgg cgtcgctggg cttttccagt
    10081 aaccagtcgt gcaaggcctg cgatatcgcg tcttccacca ctggccgcac ttcagatgac
    10141 accagcttgt ccttggtctg cgacgagaat ttcggatccg gcagcttgat cgacaggatg
    10201 cagctgaggc cttcgcgcat gtcgtcgccg gtggtttcga ccttggccct cttggcctgt
    10261 tcgttcgcct cgatatactg gttcagcgtg cgcgtcatcg ccgcgcgcaa cgcggtcaga
    10321 tgggtgccgc cgtcgcgttg cggaatattg ttggtgaagc acagcactga ttcctggtac
    10381 gaatcgttcc attgcatggc gacttccacg ccgatcccgt cgcgctcgca gatcgcgtgg
    10441 aaaatgttcg ggtgcagaac cgtcttcgag cggttgatgt actcgacgaa gcccttgacg
    10501 cccccggcga aagcgaaggt ctcttccttg gcattgcgct ggtcatgcag ctcgatgcgc
    10561 acgccattgt tcaggaagga gagctcacgc agcctttttg ccaggatgtc gtaatgaaac
    10621 tcgatgttgc cgaagacttc cggattcggg tggaaatgca cttccgtgcc gcgacgttcc
    10681 gtttgcccga cgaccttcag aggagaaacc gggtcaccgt tgcggaactc catgaaattc
    10741 acctgcccat cgcgccggat cgtcagcttc agccagtcgg acagggcgtt caccaccgac
    10801 acgcccacgc cgtgcagacc gccggagatc ttgtagctgt tgctatcgaa ttttccgccg
    10861 gcatgcagaa cggtcatgac gatttccgcc gtggaccgtc cttcctcgtc ctccggatgg
    10921 atgccagttg gaattccgcg gccgttgtcg acgacgctga tggaattgtc gccgtggatt
    10981 accaccttga tctcgtcgca gtggcccgcc agcgcttcgt cgatcgagtt gtcgagcacc
    11041 tcgaacacca tgtggtgcag tccggtgcca tcgctggtgt cgccgatgta catgccgggt
    11101 cgcttgcgga ccgcctcgag gcccttcagg accttgatgc tgtcagagtt gtattctgat
    11161 tccgacattc gtggatatcc gctttgaatt tcagatgcgc atcggcatca cgacgtactt
    11221 gaattccttg tttccgggca ccgacatcag catgctgctg ttggcgtcgc caaccgcgca
    11281 gtccacgtcc ttcgtgtcca ggttgttaag cacatccagc agataggtga tgttgaagcc
    11341 gatatcgagg gcctcgccgt tgtagtcgat gtcaagctct tcctcggcct cttcttgctc
    11401 ggcgttggtg cagacgattt tgaggctgct ggcaccgatc acccagcgca caccgcggaa
    11461 cttctcattt gaaagtattg cagcccgctg caacgcctgt tggagcagca ggcgatccag
    11521 gatgaaatgc ttcttgtagt tcgttggtat aacccgcgtg tagtcgggaa attttccatc
    11581 cacgaccttc gaaaccaggt cgatccccga aaagcggaac tgcacctggt tcggcagaat
    11641 gtcgatttta acttcgtctt cgccgtcggc gagcagcttt gccagctcca gcaccgcctt
    11701 gcgcggaacg atgacctccg accgtgctac tggttggggc agtttcgcgg ccgcgtaggc
    11761 cagcctgtgg ccgtcggtcg ccaccatggt cagctggctg tcctcgacca tcagcaacag
    11821 gccgttgaga taataacgga tatcctgctg cgccataccg tactgcacta gcagcaactg
    11881 ctgcctgagc actttctgcg acagtcgtac cgaaaccgcg ccattcccgc catctgccag
    11941 tttcggaaaa tcctgtgctg gcagagtctg caggttgaat ctgctctttc ccgcttttac
    12001 ctgcaggcgc ccttcctgcg cgtcgaggct gaccgtagtt ttatccggaa gcgagcgcag
    12061 gatatcctgc aactttctgg cagataccgt taccgcgtag cgcgacgcgg ccttctccag
    12121 tatcgcagag gtcgccacct ggatttcaag atcggtagcc aacaggttca gcgccgtgcc
    12181 atccgcttcc agcatgacgt tggaaaggat cggcagtgta tgccggcgtt ccacgattcc
    12241 ggataccgcc tgcaatggtc gcagaagtac gtctttttct atttccaaga tcttcattct
    12301 tgaaccttaa taagattaat agatcgcgga aaacctgttc gctatcgtac ttgttgcttt
    12361 gaaaacacgt acttccgatt ctgtataact cgtgtaaccg cggtggacga attgtggata
    12421 cttgccgccg atctcatgcg gtgtgattta tcaacaaact gtacagcgtt tatcaaccgc
    12481 gcaaagtctg cagcagcgcg tcgaagtcac gggtaatgtc ggcattgctc gtgcgcagat
    12541 ccgctatttt gcggcatgca tgcaggaccg tggtgtggtc gcggccgccg aaggcgtcac
    12601 cgatgtccgg cagactcagg tgcgtaagtt ccttggccag agccatggcc acctggcgcg
    12661 gccgcgcaac catgcgcgag cgcttcttcg agtacatttc agaaaccttg atcttgtagt
    12721 agtcggcgac gattcgctgg atattttcaa ccgacacctg cctcacctgc acggcgagaa
    12781 gatccttgag ggcctcgcgg gcaagatcca ccgagagcct tgtgttggcg aactttgcgt
    12841 aggcgagaac acgcttcagg cccccttcga gttcgcgtac gttggactgg atgtgcgtgg
    12901 caacgaaaaa tgcgatgtcc tccggcaggc tgacacgctc ggcttccgct tttttgagca
    12961 ggatcgcgac gcgcatttcc agttccggcg gctcgattgc aaccgtaagc ccccagccga
    13021 agcgcgagat cagcctatcc tcgattcccg cgatttcctt cgggtacgta tcgcaggtaa
    13081 tgatgacttg cttgtgcgct tcgatcagcg cgttgaaggc gtagaaaaat tcctcctggg
    13141 tgcgattctt gccgctaaaa aactggatat cgtcgatcag cagaaggtcc agcgagtggt
    13201 agtacttctt gaaaccgtca aaggccttgt gctgataggc gcgaaccacg tcggaaacgt
    13261 agcgctcggc gtgaatatag cggaccttgg cggacgggct gagttcgagc agcaggttac
    13321 cgatggcttg gatgaggtgg gtcttgccca ggccgacccc accgtagaca aacagcgggt
    13381 tgtacgccac gccaggatgt tcggagacct gcagcgcggc ggcgcgcgcc agttgattgg
    13441 ccttgccggt tacgaaggag tcgaaagtaa aggacgggct gaggcgactc tgctcccgga
    13501 tttttggttt cggcgccggc gcttccgctt ctggcacagg ctggaccgtt tcttcggcgg
    13561 cgttttccga aagagttagt actacgtgag taggtttgcg caagaaatcc gaagacagcc
    13621 gttctatttc ggagacgaat ttgtccttga cccactcgag aacaaagcga ttcggcgccg
    13681 aaaccactac cgtgtgctgg tccaggtgcg cgtgcagcgg ctgaatccag gtcttgtact
    13741 gttgcgccgg gagagatttt tcaaaatggc gtaggcagtg attccagagg ctatccatcg
    13801 gcgccgggtt gtggttttgg tacgggatgc ggccgaggaa ggccacctaa ggtgagggcg
    13861 aattttatca gcaatatttg gagttatcca caggctcgac gagatcgatt tcgcggttga
    13921 caatagcctg taaaaacaag tgtaatgacg ggctttttca caccagccac cggagtaagt
    13981 cgaatgaagc gcacttatca gccgtcgaaa acccgccgca gccgcaccca tggctttctg
    14041 gtgcgcatga aaacccgagg cggccgcgcc gtcatcaatg cccgtcgagc caagggccgt
    14101 gcgcgcctgg cggtgtgaca cagccgcgtc ccaagctgcc tcgcctggcg aggctgtgcg
    14161 gtggggcgca tttcaccggc gcattctcgg caagacgaca ggggcgattc tttatcgtcc
    14221 tcaccaggaa taatccgggc gccaagtccg cacgtttggg catcgtcatc ggccggcgtg
    14281 ccgcgcccaa ggcagttacc cgttcagcga tgaaacgtct ggtacgcgaa gtgtttcgcc
    14341 acctgcgcag cgatctcgga tccacggatt ttgtcgtgag ggtacgcagg cctgctgtca
    14401 gggaagaaat gttcgaggca cggggtgaac tgcaaagcct gttaagcggc cgtcgctgat
    14461 gcgtgccaca ttagtaaacc tgattaagct ataccaatac ctgttgagcc cgtgggtcgg
    14521 caatcactgc cgcttctacc cgacttgttc ggaatacgcg aaagccgcga tcgagaccca
    14581 cggcgtcttg cgcggaagct ggttcgccgg caagcgcctg ctgcgttgcc acccttggca
    14641 gcccggcgga gctgacccgg taccctgaat agaaaacgtc ttccatggat agtttgcgat
    14701 tgtttgcggc gatgattttc gccgtttccg ttttcctgct gttcgacgcg tgggtgaagg
    14761 aacgtaatcc gccgccgccg ccgcaggatg cggcgcaaca agccgtgact gctacaccgt
    14821 caccgacgct tccacccggt gtgacgacac cagcaagccc ggccgcgccg cctgccaaag
    14881 ctgcggcgtc aagtgccgcg aacttgcctg tgatcaccgt gcataccgat gtgctgaatg
    14941 cggaaatcga cccggttggc ggggtgttgc gcaggctcga gtttacccag cacaaggaca
    15001 aggtcgacaa gaacaggaat ttcatcctct tcgagcagtc agccgatcat acttacgtgg
    15061 cgcaaagcgg cctcattggc gacaaactgc ccaaccacac aacgccctat cgtgctgcta
    15121 atgcgaatta ccaattgagc gacggcaacg atgaagtaac cgttactttg accgcaccgg
    15181 aatccgatcg catcgccgtg actaagcgca tcactttcca gcgcggcagc tatctcatcc
    15241 acgagtcctt tcggatcgtc aacggtggca gcgacgagct gaagccgttt gcctatttcc
    15301 agtttcttcg cgacgggaaa ccggccgcag gcgattcgaa ggtggtgccg acctacactg
    15361 gtgcggccgt gtacacggac cagtccaagt tccgcaaggt aactttcagc gacatcgata
    15421 agggcaagac tgactatccc aaacatgtcg acgacggctg gatcgctttc gtgcagcact
    15481 atttcgtggc ggcgtggctc ccggccggtg aaactccgcg cgagttctat acgcgcgcgc
    15541 tgagcaacgg cttgtacgct gcgggcgtta tcgtgccggt gggcggtatc gcaccgggcg
    15601 cctcggggca agtcgacttg cgtctatacg ccggcccgca ggagcaggaa aagctcgcca
    15661 agcttgctgc gggcttggaa ctgacggtgg attacggctg gctcacggtc atcgcggcac
    15721 cgttattctg ggtgctgtcc gcgatccaga agtgggtaaa caactggggc gtggcgatca
    15781 tcatcctgac gatcatcatc aaactgatct tctatccgct gtcggccgca agttacaaat
    15841 cgatggcgaa aatgcgcgtg ctcgggccga aaatgcagaa gctcaaggag cagtacggcg
    15901 atgatcggca gcgcatgcag caggcgatga tcgaaatgta caaaaccgag aagatcaatc
    15961 cgctcggggg ttgtctgccg atcgtggtgc agattccggt gtttatcgcg ctgtactggg
    16021 tattgcttgc gagcgtggaa ttgcgcaacg cgccattcat gctctggatc accgatctgt
    16081 cggcacagga tccgtactac atcctgccgg tgctgatggg catcaccatg atcatccaga
    16141 cttcgttgaa cccgaccccg ccggatccgg tgcaggcgaa ggtgatgaaa atcatgccag
    16201 tggtcttcag cgtgttcttc ttcttcttcc cggcagggct cgtgctctac tggctggtga
    16261 acaacattct ttccatcgcg cagcagtggc agattacgcg cgccctcgag cggtcaaaac
    16321 ccgctcatgt cccgcgctga cacgattgcc gcgattgcga cggcccccgg ccgcgcggga
    16381 atcggcgtag tccgcgtttc cggctccggg gtccgcgcca tcattgcggg aattcttggg
    16441 cgtgtacccc aacctcggac cgccacactt cacaactttc acgacgcggc gggcgggacg
    16501 ctcgaccgcg gcctcgcgtt gttctttgcc gcgccgcact cctacaccgg tgaggatgtg
    16561 ctcgaacttc atgggcacgg cggggccgcc gtactgcaat tggtgctgcg acgttgtctc
    16621 gatctcggcg cgcggccggc gcagcccggc gagttcaccc gtcgcgcctt cctcaacgac
    16681 aagctcgacc ttgctcaggc ggagagtgtc gccgatttga tcgatgccag ttcggaggcc
    16741 gcagcgcgcg gagcaatgcg ttctctggcg ggcgagttct ccgctcgcat cgatcatctc
    16801 gtcagcgcct tgatcgaatt gcgtgctctg gtcgaggcga cactggattt tcccgatgag
    16861 gagagcgatt ttctggagcg gggcgacgcg ttcaagcggc tggcgcggct gcgcgcggaa
    16921 cttggcgagg ttcagcgcgc ggcggagcag ggcaggattc tgcgcgaggg cgcgagatgt
    16981 gtactggtcg gtcagcccaa cgtcggcaag tcgagtcttc ttaaccgtct cgcgggcgac
    17041 gaggtcgcca tcgtcaccga gattccaggc accacccggg atccgttgcg gcacgaactg
    17101 gtaatcgatg gcgtgccggt gcacgtgatc gacactgccg ggctgcgcga atcgcaggag
    17161 ccgattgaaa aaatcggtat cgaacgcgcg tggcgcgaaa tcggtcaggc ggaccttgcc
    17221 atgctggtgg tggacgtgac ccgggggatg gcaaacgccg atcgcgcgat ccttgcgaaa
    17281 ctgcaagaga aggtgaaaaa gatatttatt ttcaataaaa tagatctatc cggcgatccg
    17341 ccttcgctgt gcgaactgga cgaccgggtg gagatccggg tatcggccaa gtccggcgcc
    17401 gggttggaac tgttgcgcgc caacattctg cgggccgtcg gctggcgacc ggtagaggaa
    17461 atgcagttca tggccagaga gcggcacttg cgcgcgctcc aacaagccga cgcagcgctc
    17521 gcgcgtgcgg cggccgttcg ggcgaaggcg atcgaattgt ttgcggaaga actgcgactg
    17581 gctcaacagg cgctgggcga gattaccggc cagttcagcg ccgacgatct gctcggcgaa
    17641 attttctccc gtttctgcat cggcaagtag acggtcgtga tcgatgtcct tgttctgtgt
    17701 gtatttgcgt tcttcggcgg gctggtggac gcggtcgccg gcggcggtgg cctgctcctt
    17761 gtaccggcgc tgtttgcggt ctatccgtcg gcgccgcccg cgctcctgct cggcaccaac
    17821 aagctttctg cgatagccgg taccaccttc gccacgctgc gctatgcctg gtcggtgccg
    17881 attcgctggc ggacaatcct gcccgctgca ctggtagcgt gcggcggcgg ccttgccggg
    17941 gcaaaggcgg tgaccttgat cgagcccacc gtgctgcggc cgctgattct ggtgatgctg
    18001 ggagcggtcg cgatttacac gctggttcgg caaaacttcg gttccgcgcc cgacgggcgg
    18061 gtgcggctca agatcgaagg gccgggggtg gctgtattgg cggcggtcat cggtttctat
    18121 gacggattct tcggtccggg agccggcagt ttttttctgt tcggtctggt aaggctgttc
    18181 gggtacgatt ttctcgccgc cgccgccgcg accaaagtct tgaatctggc gacgaatttc
    18241 ggcgcgttga tgttgttcgt ggcgagcgac aatgtccttt acgcggtcgg attgccgatg
    18301 gcggcctcca gcatcgcggg tggattgctc ggcgcccaac tggcggttcg ccagggcagc
    18361 cgcttcatcc gctggatgtt tctgctggtc gtggcggcgc tgatcgtcaa gcttgcttat
    18421 gatttgctgc tgcgaccgta ggtgatttgt tccacgtgaa acgttcggcg ccgatgatgg
    18481 cgcatgtttc acgtggaaca tttggaggtt tcggcggtgc cggattcctc gaagtcgggg
    18541 ctttcaggta tgattccgcc ctcttttccg gcccggcaat gcagcactcc aattctttcg
    18601 acatcatcgt ggtgggcggc ggtcatgccg gcaccgaggc cgccttggcg gcggcccggg
    18661 cgggttgttg cacgctactg ctgacccaca acatcgaaac cctcggccag atgtcctgca
    18721 atccgtcgat cggcggaatc ggcaaggggc acttggtcaa agaggtggat gcgctgggtg
    18781 gcgcaatggc ggccgccacc gacgaggcag gaatccagtt tcgtatcctc aattccagca
    18841 agggaccggc ggtgcgcgcc acgcgtgcgc aggccgaccg cgtgctgtat cgccaagcca
    18901 tccgtcggcg ccttgaaaat cagaaaaatc tcacgatctt tcagcaagct gtagatgacc
    18961 tgagcctcga cggcgagcgc gtagccgggg tggtgacgca gcttggtctg cgtttctacg
    19021 cgcggacagt ggtcttgacg accggtacct tcctgtcggg actggtgcat gtcggcctgg
    19081 atcattacca ggccgggcgc gctggcgacc cgccggcaat tagtctggcg cggcggctgc
    19141 gcgaattgca gttgccggtc ggacgcctga agaccggcac gccaccgcgg cttgatggcc
    19201 gcagcatcga ctattcggta atgaccgagc agccgggcga cgatccggta ccggtgtttt
    19261 cctttctcgg ttcggccggc cagcatccgc ggcaattgcc gtgctggatt acccacacca
    19321 atttgcgcac ccatgaaatc atccgtggcg ggctcgatcg ttcaccgatg ttcaccggca
    19381 ttatcgaagg cgtcgggcct cgttactgcc catccatcga ggacaaagtc acgcgcttcg
    19441 ccgaaaagaa ctcgcaccag atctttctcg aacccgaggg cctgaccacc aacgaggtct
    19501 atccgaacgg catttcgacc agcttgccgt tcgatgtaca agcagaatta gttcattcga
    19561 tcaagggttt ggaaaatgca cacctgaccc gcccaggcta cgccatcgaa tacgactact
    19621 tcgatccgcg aggactgaag tcgtcgctgg aaaccaaggc gatcaagggg ctgtttttcg
    19681 ccggccagat caacggcacc accggctacg aagaagcggc cgcccaaggc ctgcttgccg
    19741 gaatcaatgc agcgctgctg gtacaaggca aggaaccttg gtgcccgcgg cgtgaccagg
    19801 cttacctcgg cgtgcttgtg gacgacctga ttactcgcgg cgtgaccgag ccctatcgga
    19861 tgtttaccag ccgtgctgaa taccgcttga gtttgcgcga agacaacgcc gacatgcgtc
    19921 tcacggaaat cggcaggcgc cttggcgtgg tagacgatgc gcgctgggag ttcttcgaac
    19981 acaaacgcga agtggtggcg cgcgaattgg agcgccttaa gacgacatgg gtcaacccga
    20041 agcagctggc cgaagaaacc atgctgcaac tgttcggaca gcctttggat cgcgactaca
    20101 cattggagga gctgctgcgc cggccgtcgg tttgttatcg cgacctgatg gcgttgaccg
    20161 ctgcgggacc cggtgtgacg gaggatcaag ctgcggaaca ggtcgaagtc cagaccaagt
    20221 atcggggata catcgagcgc cagcgtgaag aggtcgagcg gcaggagcac ttcgaatccc
    20281 tgtgcctgcc cgcgaatctc gattatcgca gcgtgcgggg actgtcgatc gaggtgcaac
    20341 agaaactcaa caagcacaag cctgagaccc tgggccaggc cgcgcgcata tccggcatca
    20401 ctccggcggc gatttcgctg ctgctggtgc acttgaagcg cggcatcaag gcgacagccg
    20461 aggctggcgc ccgccacggc agccaggtac gcgccgcggg aaaccgcggt tgagccaggc
    20521 ggctgagctg ttgcggcagg gtattgcaga cctggatttg gcgttgccac agttcgcgac
    20581 cgaacgccta ctcgactacc tggtactgct ggcgaagtgg aatcgcgttt acaacctcac
    20641 ggcaatccgt gaggaaa
//
LOCUS       DASQOP010000359         2859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_3005930, whole genome shotgun sequence.
ACCESSION   DASQOP010000359 DASQOP010000000
VERSION     DASQOP010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3005930"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1328
                     /locus_tag="VE030_13930"
     CDS             <1..1328
                     /locus_tag="VE030_13930"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HZF24554.1"
                     /translation="LITFVSGAILWVLTRSSQAELQTRDGFLLVTLVWTVLPAFATLP
                     LLTYMPDLSFTDAYFETVAALTTTGSTVLSGLDKLPPSINLWRCELQWLGGMGIIVLA
                     VAILPLLGIGGSQIMRTETPGPMKDTKLTPRITETAKGLWLVYAIMTAACILAFKAVG
                     MGWFDAVVHSFSTLSLGGFSSHDASFAYFNSPAIEATAIFFMLIGGINFGTHFLAFRR
                     LSFAPYRSDPEARAYVAVLTLSVLGVAYFLFANHVYPSFWTALRHSAFNVVSVATTAG
                     FASTDFNQWPIFAPVWMLFLCCFATCSGSTGGGIKMIRAELMVRQALREIMSIIHPRA
                     YLPVKLAGQRVENNIIFAVLAFMLIWGGSVIAMTMLLVASGLDIITAFSAVIVCICNT
                     GPGLNQVGPATTYAALSDFQTWVCTVAMLIGRLELFTLFVVVTPAFWRK"
     gene            complement(1337..1684)
                     /locus_tag="VE030_13935"
     CDS             complement(1337..1684)
                     /locus_tag="VE030_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZF24555.1"
                     /translation="MSIKRHQAGKLFSAAVEHGGLVYVAGQVADDLTQGAKGQTEQVL
                     KRIDAILAAAGTGKSKILSANVWVTDIRNRDEMNAAWTAWADPANLPARATVEAKLAD
                     PRMLVEIMVTCAK"
     gene            complement(1698..2390)
                     /locus_tag="VE030_13940"
     CDS             complement(1698..2390)
                     /locus_tag="VE030_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZF24556.1"
                     /translation="MEYVISSWEIPSLPIAGSNARFPVRRIFCVGRNYVEHQKEMGGD
                     GREQPFFFTKSAHDLVPEGGKVHFPPMTSNYHWETEMVAVVGTGGYKIPAAKANDHVW
                     GYAVGLDMTRRDLQQVGKDKGRPWTFGKDFDQSAPVGPITPASKSGHPKKGKLWLKVN
                     GELRQNSDIDQMIWSVPEQIAFLTQYYSLEAGDLIMTGTPAGVGAAKPGDELLAGIEG
                     LGELRVKILPPR"
     gene            complement(2557..>2859)
                     /locus_tag="VE030_13945"
     CDS             complement(2557..>2859)
                     /locus_tag="VE030_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HZF24557.1"
                     /translation="HAVGGILGALLTGVFNSPTLGGPSFPVDWFTGKITTAAEYSILG
                     QLWIQAKAVGVVFVWTGVVAFISFKIADLLVGLRVTEEEEREGLDINSHGETAYRM"
BASE COUNT          470 a          923 c          867 g          599 t
ORIGIN      
        1 tgctgattac ctttgtgagc ggagcgatat tgtgggtgct cacccgctcc agccaggccg
       61 agctgcagac ccgcgacggg ttccttctgg tgacgctggt gtggacggtg ctgcccgcgt
      121 tcgccacgct gccccttttg acctacatgc ccgatctcag tttcacggac gcctacttcg
      181 agacggtcgc ggccctgacc accacgggtt cgaccgtact atcggggctc gacaagctgc
      241 cgccgtcgat caatttgtgg cgttgcgagc tgcagtggct gggaggcatg gggattatcg
      301 tcctcgcggt cgcgatcctg ccgctgctcg ggatcggcgg cagccagatc atgaggaccg
      361 agacccccgg gccgatgaag gataccaagc tgactccgcg catcaccgaa accgccaagg
      421 ggctgtggct ggtgtacgca atcatgacgg ccgcctgcat cttggcgttc aaggccgtgg
      481 gcatgggctg gttcgatgcc gtcgtgcatt cgttctcgac cctgagcctg ggcggatttt
      541 cctcccacga cgcgagcttc gcttacttca attcaccggc gatcgaggcc acggcgattt
      601 tcttcatgct catcgggggc atcaatttcg gaacccactt cctcgcgttc cgcaggctga
      661 gcttcgcgcc ttaccggagc gaccccgaag cgcgcgccta cgtcgccgtg ctcacgctgt
      721 cggtcctggg ggtcgcctat ttcctctttg ccaaccacgt ctacccgtct ttttggaccg
      781 cgctgcgcca cagcgcgttc aacgtcgtct cggttgcgac gacagccggg tttgcgagca
      841 ccgatttcaa tcaatggccg atcttcgcgc cggtgtggat gctgttcctg tgctgcttcg
      901 ccacctgttc cggctctacc ggaggcggca tcaagatgat tcgcgccgaa ctgatggtcc
      961 ggcaggcgct ccgggagatc atgtccatca ttcacccgcg cgcatatctg ccggtcaagc
     1021 tcgcaggcca acgggtggag aacaacatca tcttcgcggt gctggcgttc atgttgatct
     1081 ggggcggatc ggtgatcgcc atgaccatgc tgctggtggc ctcggggctg gatatcatca
     1141 ccgctttttc ggcggtgatc gtctgcatct gcaatacagg gccggggctg aaccaagtcg
     1201 ggccggcgac gacctacgcg gcgctcagcg attttcagac ctgggtatgc acggtcgcca
     1261 tgctgatcgg acgactggag ctgtttacgc tgttcgtcgt cgtcacgccg gctttctggc
     1321 ggaaatagct tgcgccctac ttggcgcagg tcaccatgat ctcgaccagc atgcgcgggt
     1381 cggcgagctt cgcttcgacc gtggcgcgcg ccggcagatt ggcgggatcg gcccacgccg
     1441 tccacgcggc gttcatttcg tcgcggttgc ggatatcggt gacccacacg ttggcggaaa
     1501 gaatcttcga cttgccggtt cccgcggcgg cgaggatggc gtcgatcctc ttcaacacct
     1561 gctcggtctg gcctttggcg ccctgggtga gatcgtcggc aacctggcct gccacgtaga
     1621 cgaggccgcc gtgttcgact gcggcgctga acagtttgcc ggcttgatgg cgtttgatgc
     1681 tcacgtcgac ctcccgttta tcgcggcggc aggattttca cccgcagctc gcccaggccc
     1741 tctattcccg cgaggagctc atcccccggc ttggcggcgc ccacgccggc cggtgtgcct
     1801 gtcatgatga gatcgcccgc ttccaggctg taatactgcg tgagaaatgc gatctgctcg
     1861 ggcacgctcc agatcatctg gtcgatatcc gagttctggc gcagctcacc gttcaccttg
     1921 agccagagct tgcccttttt cggatgcccg ctcttggacg cgggggtgat cggcccgacc
     1981 ggagcggact ggtcgaaatc cttgccgaag gtccagggcc tgcccttgtc ttttcccacc
     2041 tgctgcagat cgcgccgggt catgtcgagg cccaccgcgt aaccccagac gtgatcgttg
     2101 gccttggcgg ccgggatctt gtagccgccc gtgccgacta ccgcgaccat ctcggtttcc
     2161 cagtggtagt tgctcgtcat cggcggaaaa tgcactttcc caccctcggg caccaggtcg
     2221 tgagccgatt tggtgaagaa gaacggctgc tcgcggccgt ccccgcccat ttccttctgg
     2281 tgctcaacat agttgcggcc gacacagaag atgcggcgca cgggaaagcg cgcattcgag
     2341 cccgcgatgg gcagcgaagg tatttcccag ctgctgataa cgtattccat tttcatgacg
     2401 agaggtcctc acaagatcgc aagaagtgcg gcgcgaaccg aaccgccggg cgccggcgaa
     2461 aggggcgtat tctcgcatga actgccggcg caaagaaaaa cgggcacccg aaggtgcccg
     2521 ttcgaaaacc agcccgtttc cgggcttctc cctgcgttac atgcgataag cggtctcgcc
     2581 gtgcgagttg atgtccagtc cctcgcgctc ctcttcctcg gtgacgcgca gtcccacgag
     2641 gaggtccgca atcttgaagg agatgaaggc caccacaccc gtccacacga ataccacccc
     2701 gactgccttg gcctggatcc agagctgtcc caggatcgag tactccgccg cggtagtgat
     2761 cttgcccgtg aaccaatcga ccggaaaact cggcccaccc aaggtcggcg agttgaacac
     2821 tccggtgagc agagcaccca ggatcccccc gaccgcgtg
//
LOCUS       DASQOP010000360        14115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_190179, whole genome shotgun sequence.
ACCESSION   DASQOP010000360 DASQOP010000000
VERSION     DASQOP010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14115
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190179"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VE030_13950"
     CDS             complement(<1..385)
                     /locus_tag="VE030_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24558.1"
                     /translation="MIEDRIEVNIAGGLDIELPRMVDVRQKFDAVHLGDIAATVANEF
                     QRPEVRARVKAGQAIAVGCGSRGIANIATIVKCVIRELQALGARPFIFPAMGSHGAAT
                     AEGQRKVLEGYGITEASTGVPVRATM"
     gene            complement(398..1681)
                     /gene="larA"
                     /locus_tag="VE030_13955"
     CDS             complement(398..1681)
                     /gene="larA"
                     /locus_tag="VE030_13955"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HZF24559.1"
                     /translation="MRLDLNYGRGSYPLDLPDHWDITVIRKPAMPVQPDARGAVRTAL
                     SQPVDARPLTEEARGRKTACILICDITRPVPNGLLLPEIVRQLLDSGMKPENITVLVA
                     TGLHRPNEGAELEELIGDSWVLNTVNVANHFARNDADHVDLGPTPRGTPVKLDRRFVE
                     ADVRIATGLVEPHFMAGFSGGRKVIAPGVAHQDTITTFHSARFMSHPKADNCVLEGNP
                     LHEEQLEIVRKLGHVLAVNTVIDEHRRLAFVNYGEIVASHLDAVAFIRSYAQVTVKRR
                     FNTVVTSAAGYPLDTTYYQTVKGMVGPVNILEPGGNLLVVSECSEGLGSEEYADAQRR
                     LIGLGADGFFADISRKQFADIDEWQTQMQLKPMKAGSVHLYSGGLRAGDRGLTGVNLI
                     DSVEEALHRCVEASGDTSVAVIPEGPYVVPMYQPA"
     gene            1780..2505
                     /locus_tag="VE030_13960"
     CDS             1780..2505
                     /locus_tag="VE030_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24560.1"
                     /translation="MNLSDPVMAALIGAGATFATAIFQLTSNARRQAAERAAGKPASR
                     KTGSWLAIFALVLASLVTGYALSEYQTYSGRQDEKLLRDELLARVRDISAAAVRLERA
                     GTEKNGPSGADARLAVERRLGAEGVAAIVSVPPCRTAKAGSAQAPAACSENEALRATL
                     CAVVPASATITEVQLFTRTEDSQQPWDEARVQVGQDAGAAKFVDAYIERAQGKESKEI
                     CQHFAHWNSQKGRSARILVKYTL"
     gene            complement(2646..3158)
                     /locus_tag="VE030_13965"
     CDS             complement(2646..3158)
                     /locus_tag="VE030_13965"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZF24561.1"
                     /translation="MKTLPPLDRVAIRPLADDAEAELCARMMELSEPWMSLGTGYRTL
                     FDLMRNPSRERYAAWLDNRIAGFVVINMSGTLAGYIQSICVAPGNRASGIGRLLMAHA
                     ETRIFRDTPNVFLMVSDFNAAARRFYERLGYRTIGEIPEYLVAGRSEILMRKTRGPIR
                     GHPASSRDPG"
     gene            3292..4458
                     /locus_tag="VE030_13970"
     CDS             3292..4458
                     /locus_tag="VE030_13970"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HZF24562.1"
                     /translation="MSRIKRVEVYEFAFELPDLGWDSSGFNIVYQPNNKLNLSKYAVA
                     IEDDDGARGEYVALWGGTRMAIGQTLFLAQHLIGRDPHQRELIYDDFKRALRQYDHMG
                     AGCIDIALWDLAGKQLQAPIWKLLGGWRKRLPAYASTYHGDRNGGLSSAQAYVDFAEQ
                     CYEMGYRAFKMHGWCDGNAQQESAAVLALGNQLRGRMTLMLDPACELRTFADTLAVGR
                     ACDEAGFFWYEDPMRDGGWSQFAHRKLRQFIKTPLLQTEHVRGLEPKADFIVNDGTDF
                     VRADPEYDLGITGTMKIAHLAEAFGLDVELHAPGPAHRHCMAAIRNTNFYELALVGPK
                     CGNAMPPVYAEAYTDQLDGVGKDGCFPVPDGPGLGVTYDWDFIKRHRTQLHEFK"
     gene            4691..5128
                     /locus_tag="VE030_13975"
     CDS             4691..5128
                     /locus_tag="VE030_13975"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZF24563.1"
                     /translation="MNAPYDPALIAAAGAEAADAPVEGFSLMPPQSFEADRISTQPRA
                     VTQAVDFIHDNYAGGLSLSDIAAAAHLSPFHLSRIFKKATGVSPHQYLLQVRVNSARS
                     LLAAGAGGRSLAEVAAAVGFADQSHLTRHFKRMLGVTPRQMRQ"
     gene            5220..5900
                     /locus_tag="VE030_13980"
     CDS             5220..5900
                     /locus_tag="VE030_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HZF24564.1"
                     /translation="MPLDPLQLTGRSRSHLFQSDEPRFAIHANMVEPFARLREAAAME
                     GFDIVVFSGFRDFESQAEIWNQKFCGERTLFDADGGALDYRALSEAERVQCILNWSAL
                     PGASRHHWGTDIDVYDRAALPEGYRVKLLPEEVGPGGVFHRLHGWLDQNMARFGFFRP
                     YREYRGGMFPEAWHLSYAPIAMPALEVLTLDVVAQALRDGDILGKDLVLERLPEIYQR
                     HVLNISMP"
     gene            6002..6322
                     /locus_tag="VE030_13985"
     CDS             6002..6322
                     /locus_tag="VE030_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24565.1"
                     /translation="MRNLIKRSLVVAALTAAMAGQSVLAEEQLPKDRAGGDMFLDAVF
                     VRPMGLVAIVGGAVGFVVALPFTIVSGSVDSAANELVKKPIAYTFRRPLGQIEEQEDV
                     KYSR"
     gene            complement(6374..7285)
                     /locus_tag="VE030_13990"
     CDS             complement(6374..7285)
                     /locus_tag="VE030_13990"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZF24566.1"
                     /translation="MSDLDPERRNAGVALALLSLIWGYNWVAMKTGLADAGPLQFATL
                     RFTLAALCMIPVIMRFRAPLLPRREQWPAVAILGFMLAGNFACTLAALRLGGTGKTAV
                     LVYTMPFWVLVFAWLALRERLDRWQGIAVAIALAGLVVLVEPWTLSGGMLASVLALGA
                     GMSWGASVVYVKNLQKKTSVSMLMITLWQMIVGSLLLALGWAWLEPQPVRWTVGLGLA
                     LGYTAVLATGVAWMLFYYALRRMPAGMVGLGTLATPVIGVLAAWLQLGERPAPLEATG
                     MALIGTALAMLAWLPRANAPPAGTHRR"
     gene            7471..9144
                     /locus_tag="VE030_13995"
     CDS             7471..9144
                     /locus_tag="VE030_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HZF24567.1"
                     /translation="MIWSQVYDPFGNTILSTIVCAIPVVVLLGGLGIFQIRAHYAAIA
                     GLVAAALIAMFVIGMPGGLTLRAGFYGALFAVTTICWIIFNLIFLYRMTLKAGLFKIL
                     QDSIGAVTNDRRLQLVLIAFCFGAFFEGAAGGGTPVAVTGAILIGLGFNPLAASGLSL
                     IANTAPVAYGGLGTPIIVLHSVTQPDNADYLLTLSAMVGRQLPFFSVIVPVWLVLTFC
                     GWRRTLEVWPAILVAGVSFAISQFLISNYHGPWLVDMLGAMISMLILAVFLAYWKPKN
                     VMLDASGGGVTVPAGEAAKVHHGHGRDMVIKAWTPWVILTVILFLWGLPIVKAFLNVK
                     GITYWEWQIPGLHNVVQRVEPAVAVPRVEGAVWKWGIISATGTGILLAAIIGGLVMGF
                     KPREMFRTWIETFGIMKYSLLTILAMLALGYTTRYSGMDGTLGLAFANTGWLYPFFGT
                     MIGWIGVALTGTDAASNALFGSQQVITANKLGLNPTLMAAANSSGGVMGKMIDAQSIV
                     VASVATNQQGREGVILRFVFWHSIALAALVGVLVMLQAYVPFFQAMVLK"
     gene            9326..9784
                     /locus_tag="VE030_14000"
     CDS             9326..9784
                     /locus_tag="VE030_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZF24568.1"
                     /translation="MIEQKLASLGIVLPAAAVPAFQYVAVTVHERLAFVSGQLPREGD
                     EVRIVGKVGGEVGVERAQEAARLCILQGLACLKQALGSLDRIERILKVTGFVASAPMF
                     NQQPKVIDAASELLVQVFGESGRHARSAIGVAELPRNACVEIEIIVALRP"
     gene            9917..12349
                     /locus_tag="VE030_14005"
     CDS             9917..12349
                     /locus_tag="VE030_14005"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZF24569.1"
                     /translation="MPTSENILAILRSAGIVENSQSEETYREVHAQQIALLFRQLPAA
                     LTATLLIAFIVAGVTWPVADANLLFSWLIAVCALTGHRFLLWRRFAANAPRAEQMERW
                     SRSFFVGTLLSGMVWGAGSVLLAGEDLIHQFFFGLLLAGLAAGGVSTLSSYRGVYAAF
                     LLPSLVPLAVRMALYLDPVHVAMASMMLLFAALMLGISARIHATIAESLRLRFRNLGL
                     VDTLSATKGNLEQANTALQREIGEHRKAQEILMKSEQKLRLHAHQAPLAFIEWDLSFR
                     VVEWNPAAERIFGYSRQEALGRHAASLVAAERSCDTIAALWKRLLIDKHSAQVTIDNR
                     TRDQRNIVCEWYYTPLVDGQGKVLSVMTLAQDVTANRQAQERLNYLAYHDELTGLPNR
                     SLYNDRLSQAMIEARRQGRYVGVMLLDIDHFKMVNDTLGHEAGDELLRDFGKRLSVCV
                     RESDTVARFGGDEFALVLADMADPRDAIFVAQKMLDSFAPPFFVGGRELFVGPSIGIT
                     IYPLDSDDLDGLVKNADSALYHAKAQGRNNFQFYSAELTARAQSRLDTETSLRRALER
                     REFLLHFQPKFSLETGEMTGVEALIRWQHPELGMVSPQDFIGIAEESGLILPIGEWVL
                     RAACQQVKRWHDEGLAQVRLAVNLSTKQFRHSRLKEMLAGVLAETGFDPRFLELEITE
                     SMLMENSAAVSEVLDDLKAMGISISVDDFGTGYSSLSYLKRFPIDALKIDRSFVRDVP
                     ADHDDAAIVRAIIAMSRSLRMKVIAEGVETQEQQQFLRAEGCDEIQGFLSGRPMPPDE
                     LVQRLRSRPASD"
     gene            12488..13528
                     /locus_tag="VE030_14010"
     CDS             12488..13528
                     /locus_tag="VE030_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HZF24570.1"
                     /translation="MKYNRLGSSDLNVSEICLGTMTWGEQNSEKEAHQQLHYAVSRGI
                     NFIDAAEMYPVPPKAETQGRTESYLGTWLRRQQRDKVIVATKITAPGRGFNWVRGGPK
                     AVNRKSIQEALNGSLKRLQTDYVDLYQIHWPDRYLPLFGNIFYDPKQERPTVPIEDQL
                     EAIAEFIKAGKIRYLGLSNESPWGALEFIRHARQKGLPMVASTQNAYNLLNRTFETGL
                     SEVTRREGVPLLAYSPLAFGHLSGKYLNGAKPEGARMTRYPPFGQRYDKPNVMPAVAA
                     YAELARRAGLSAAALAIGFVRSRWFCASVIIGATTPAQLRENIDTENLSLGDAVLADI
                     DAVHLRYPNPAV"
     gene            13640..13724
                     /locus_tag="VE030_14015"
     tRNA            13640..13724
                     /locus_tag="VE030_14015"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13674..13676,aa:Tyr,seq:gta)
     gene            13826..13899
                     /locus_tag="VE030_14020"
     tRNA            13826..13899
                     /locus_tag="VE030_14020"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13858..13860,aa:Gly,seq:tcc)
     gene            13975..14049
                     /locus_tag="VE030_14025"
     tRNA            13975..14049
                     /locus_tag="VE030_14025"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14007..14009,aa:Thr,seq:ggt)
BASE COUNT         2624 a         4346 c         4484 g         2661 t
ORIGIN      
        1 ccatggttgc ccggaccgga acgccggtgc tggcttcagt gatgccgtaa ccctccagca
       61 ccttcctctg gccttccgcg gttgccgcgc cgtgactacc catcgccggg aagatgaacg
      121 gcctggcgcc aagcgcctgc agctcgcgga tcacacactt cacgatcgtc gcgatattgg
      181 cgatgccacg gctgccacac cctaccgcaa ttgcctggcc ggccttgacc ctggcgcgca
      241 cctcgggacg ctgaaattca ttcgcaaccg tggccgcaat gtcgcccaga tgcacggcgt
      301 cgaacttttg ccggacgtcg accatgcgcg gcagttcgat gtccagtccg ccggcaatgt
      361 tgacctctat cctgtcctcg atcatctcta tttcccttta tgcaggctga tacatcggca
      421 ctacgtaagg cccctcgggg atcacggcga ccgacgtgtc tccgcttgct tccacgcatc
      481 ggtgcagcgc ctcctccacc gagtcgatca gattcacccc ggtaagcccc cggtccccgg
      541 cgcgcaatcc gccgctgtaa agatgcacgc tcccggcttt catcggcttc aactgcatct
      601 gcgtctgcca ctcgtcgatg tcggcgaatt gcttgcggct tatgtctgcg aagaaaccgt
      661 ctgcgcccaa gccgatgaga cggcgctgcg catcggcata ttcttccgaa cccagtcctt
      721 ccgaacactc ggagaccacc agcagattac ctcccggttc gagaatgttc accgggccga
      781 ccatgccttt tacggtctgg taataagtgg tgtcgagtgg atagccggcg gcgctggtga
      841 ccacggtatt gaagcggcgc ttgaccgtta cttgggcgta gctccggata aacgcaactg
      901 cgtcgaggtg gctcgcaacg atctcgccgt agtttacgaa cgccaggcgg cgatgctcgt
      961 cgatcacggt gttcaccgcc agcacatggc cgagcttgcg tacgatctcg agttgctctt
     1021 cgtgcagcgg attgccctcg agcacgcagt tgtccgcctt cggatgcgac atgaagcggg
     1081 cgctgtggaa ggtcgtgatc gtgtcctggt gcgccactcc cggcgcgatc actttgcgcc
     1141 cgcccgagaa gccggccatg aagtgcggct ccaccaggcc ggtggcgatt ctcacgtcag
     1201 cctctacgaa gcggcggtcc agcttcaccg gcgtgccgcg cggcgtcggt ccgagatcga
     1261 catggtcggc gtcgttgcgg gcgaagtggt tcgccacatt tacggtgttc agcacccagg
     1321 agtcgccgat cagttcttcc agttccgcgc cctcgttggg acgatgcagg ccagtggcca
     1381 ccaggacggt gatgttttcc ggcttcatgc cggaatcgag cagttgccgg acaatttccg
     1441 gcagcagcag gccgttcggt accggccggg tgatgtcgca aatgagaata caggcggtct
     1501 tccttccccg tgcttcctcg gtcaaagggc gggcatcgac cggctgggac aatgccgtgc
     1561 gcacggcccc ccgcgcgtcg ggttgcaccg gcattgccgg cttgcggatc acggtgatgt
     1621 cccaatggtc aggcagatcg agcggataac tgccacggcc ataattgaga tcgagtcgca
     1681 tggcgggaga ttgcgtacga gaattgcttg gactatagca ccggccggga tggcagccta
     1741 cttccggcgg aacaacctag gcggtagcat cgtatggtca tgaatctctc cgacccggta
     1801 atggcggcgc tcataggcgc cggcgcaacg ttcgccaccg cgatattcca actgacgtcc
     1861 aacgcgcgca ggcaggccgc ggaacgggcc gcaggcaaac cggcttcgcg caagaccggc
     1921 tcctggctgg cgatcttcgc cctggtgctg gcttcgctgg tgaccggcta cgcgctttcg
     1981 gagtatcaga cttacagcgg gcgtcaggac gagaaattac tgcgcgacga attgctggcg
     2041 cgggtgcgcg acatcagtgc cgccgcggtg cggctggaac gcgccggaac ggaaaaaaac
     2101 ggcccgtccg gggccgatgc gcgattggcc gtggagcgcc ggcttggcgc cgaaggcgtg
     2161 gccgcgatcg tcagtgtgcc gccttgccgg acagcaaagg ccggttctgc gcaggcgccg
     2221 gcggcatgtt ccgaaaacga ggccttgcgc gcgacgctgt gcgcggtggt gccggcgtcg
     2281 gccacgatca ccgaagtgca gttgttcacg cggaccgagg actcgcaaca gccttgggat
     2341 gaagcgcggg tgcaggtggg acaggacgcc ggcgcggcaa aattcgtcga cgcctacatc
     2401 gagcgcgcgc agggaaagga atcgaaggaa atctgccagc actttgcgca ctggaacagc
     2461 cagaagggcc gcagcgcgcg catcctcgtc aaatacactc tctagcttgg cgcaagcagc
     2521 gacagtgcca ttccggcaat gcctcaagct gcaatttgaa gtttccgatg gtgctgtctc
     2581 tgccggccaa tgctacggcg tgagcatgcc tcgcgcccgg cgcgtcgcac cattacccgg
     2641 acaagtcagc cgggatccct ggagcttgcc gggtgtcctc gaatcgggcc gcgggtcttg
     2701 cgcatcagga tttcggaacg cccggcaacc agatactccg gaatttcccc gatggtgcgg
     2761 tagccgagcc gttcgtagaa tcggcgtgcc gcggcgttga aatccgagac catcaggaaa
     2821 acgttcggcg tgtcccgaaa gatgcgcgtc tcggcatgcg ccatcagtaa cctgccgatt
     2881 cccgaggccc gattcccggg cgcaacgcag atgctctgta tgtagcccgc cagcgtaccg
     2941 gacatgttga tcaccacaaa gccggcgatc ctgttgtcga gccacgccgc gtagcgctcg
     3001 cgcgaggggt tgcgcatgag atcgaacaat gtgcggtagc cggtgccgag cgacatccac
     3061 ggctcggaaa gctccatcat gcgtgcacaa agttctgctt ccgcgtcgtc cgccagcggc
     3121 cggatggcga cccgatcgag cgggggcaat gtcttcattg gcgtgggtgc attgctgagc
     3181 cgtgtttgat gccgggcatt atgccgtgcg ttgacgcgcg aggtatcggc tgactatgat
     3241 cgccagaaaa acgcgtgcca ccgcatcagc ccggtcgtca aaggaaactc catgagtcgc
     3301 atcaagcgcg tcgaagtcta cgagtttgca ttcgaactgc ccgatctcgg ctgggacagc
     3361 agcggtttca acatcgtcta ccagcccaac aacaagctga acctgtcgaa gtatgccgtg
     3421 gcgatcgagg acgacgacgg cgcgcgcggg gaatatgtgg cgctctgggg cggcaccaga
     3481 atggcgatcg gccagacgct gttcctggcg cagcatctta tcgggcgcga cccgcaccag
     3541 cgcgaactga tctacgacga tttcaagcgc gcgttgcgac agtacgacca catgggcgcg
     3601 ggctgcatcg acatcgcgct ctgggacctg gcgggcaagc agttgcaagc gccgatctgg
     3661 aaactgctgg gcggctggcg caagcggctg ccggcttatg cgtcgacata ccacggcgat
     3721 cgcaacggcg gcctatccag cgcgcaggcc tacgtggact tcgccgagca gtgctacgaa
     3781 atgggctatc gcgccttcaa gatgcacggc tggtgcgacg gcaacgcgca gcaggagagc
     3841 gcggcggtgc tggcactcgg caaccaactc cgcggccgca tgaccttgat gctggatccg
     3901 gcctgtgagc tgcgcacctt tgccgatacg ctggccgtcg gccgcgcctg cgatgaggcc
     3961 ggctttttct ggtacgagga tccgatgcgc gatggcggct ggtcgcagtt cgcgcaccgc
     4021 aaactgcgcc agttcatcaa gacaccgctg cttcagaccg agcacgtgcg cggcctggaa
     4081 ccaaaagcgg acttcatcgt caacgacggt acggatttcg tacgcgccga tccggaatac
     4141 gatcttggca ttaccggcac gatgaagatc gcgcacctgg ccgaagcctt cgggctcgac
     4201 gtcgagctgc atgcaccagg cccagcgcat cgtcactgca tggcggcaat ccgcaacacc
     4261 aatttctacg aactggcgct ggtgggcccg aagtgcggca acgccatgcc accggtgtat
     4321 gcggaggctt acaccgacca gctcgacgga gtcggcaaag acggttgctt cccggtgccc
     4381 gacggcccgg gcttgggtgt gacctacgac tgggatttca tcaagcgcca ccgcacccag
     4441 ttgcacgagt tcaagtagga cggcggctct cgccaaatct gcggcgctgg ctggattgcc
     4501 gctttgatgc gctgcccgtg cgaccccaaa ggcctgttga cggtgccggc gtgccccgat
     4561 tatattactg accagtcagt tactttctgg ccgcagggcg aacaaaccag aggatccgac
     4621 cggtcatgaa cgagggcacg tcatggtgcg caagccattc acgcaccgaa ctgttccagg
     4681 ggaaaagaaa atgaatgcac cttacgatcc ggcattgatt gcggctgcgg gcgcggaagc
     4741 ggcggacgcg ccggtggaag gtttcagcct gatgccgccg cagtccttcg aagccgacag
     4801 gatttccacc caacctcggg cggtcaccca ggccgtcgat tttatccacg acaactacgc
     4861 cgggggcctg agcctgtcgg acatcgcggc cgcggcgcat ctgagcccat ttcatctttc
     4921 gcggatcttc aagaaggcca ccggcgtctc gccgcatcag tacctgctgc aggtgcgggt
     4981 gaacagcgcc cgctctctac tcgccgctgg cgccggcggc cggtcgctgg cggaagtcgc
     5041 ggccgcagtc ggatttgccg accagagcca tctcacgcgg cacttcaagc gcatgctggg
     5101 cgtgacgccc aggcaaatgc gccagtaaat cacggcagcc ctggagcccc gcgcggggtg
     5161 gtgtttcggt agacggattt gcgacaatcc gccttcgaca acgccgccgg cgtcgtgaca
     5221 tgccgcttga tcccctgcaa ctgacgggcc gctcccggtc ccacttgttt cagtcggacg
     5281 aaccgcgttt cgccatccac gcgaatatgg ttgagccgtt tgcgcgattg cgcgaggcgg
     5341 cggcgatgga agggttcgac atcgtcgtgt ttagcggctt ccgcgatttt gagtcgcagg
     5401 ctgaaatctg gaaccagaag ttctgcggcg aacgcacgct tttcgatgcc gacggcgggg
     5461 cgctcgacta ccgcgccctg tccgaagcgg agcgggtgca atgcatactc aactggtcgg
     5521 cgctgcctgg cgccagccgc caccattggg gtaccgacat cgacgtctac gatcgcgcgg
     5581 cccttccgga gggctaccgg gtgaagctgc tgccggagga ggtggggccg ggtggtgtct
     5641 tccatcggct gcacgggtgg ctggaccaga acatggcacg cttcggtttc ttccgccctt
     5701 accgtgaata ccgtggtggc atgtttcctg aggcgtggca cctgtcctat gcgccgatcg
     5761 cgatgccggc gctggaggta ttgaccttgg atgtggtggc gcaggcgctg cgcgacggcg
     5821 acattctggg caaggacctg gtgctggagc gtctgcccga gatctaccag cgccacgtcc
     5881 tcaatatttc catgccgtga acgctggggc gcaagcgctg ttgtcagacc ttgtgtttcc
     5941 ggcaccgaaa gaaagggtta caatgcattc ctcgactagc ttaccaaaat ggaggaaagc
     6001 gatgcgtaac ctcatcaagc gcagcctagt ggtcgccgca ttgactgccg cgatggcagg
     6061 acaatcggtg ctggcggaag aacaactccc gaaagatcgt gccggcggcg acatgttcct
     6121 ggacgcggtg ttcgtgcggc caatgggctt ggtggctatc gttggcggtg cggttggatt
     6181 cgttgtcgcg cttccgttca cgatagtcag cggcagcgtt gattcggcgg ccaacgagct
     6241 ggtcaagaaa cccatcgcgt acacgttcag gcgcccgctc gggcagatcg aggaacaaga
     6301 agacgtgaag tattcgaggt gaaggcaggt caagcgttca aacctgggag aactccagga
     6361 acaagagtag cgttcagcgc cggtgcgtgc cggcgggcgg tgcgttcgcg cgcggcaacc
     6421 acgccagcat ggcgagcgcc gtgccgataa gcgccattcc tgtggcctcc agaggcgcgg
     6481 gccgttcccc caattgcagc catgcggcca gcacgccgat caccggcgtg gcgagcgtgc
     6541 ccaacccgac cattccggcc ggcatgcgcc tgagcgcata gtagaagagc atccacgcca
     6601 ccccggtggc cagcaccgcc gtgtagccga gagcgaggcc taagcccacc gtccagcgga
     6661 ccggctgagg ttcgagccag gcccagccca gtgccagcag caacgagccg acgatcatct
     6721 gccacagcgt aatcatcagc atcgaaacgc tggttttctt ctgcaggttc ttcacgtaca
     6781 ccacgcttgc gccccaggac atgcctgcgc cgagcgcgag cacgctggca agcattccgc
     6841 cggaaagcgt ccagggttcg accagcacca ccagtcccgc cagggcgatc gcaactgcga
     6901 tcccctgcca gcggtcgagc cgctcgcgca gggccagcca cgcgaacacc agcacccaga
     6961 acggcatggt gtagaccagt accgcggtct tgccggtgcc gcccagccgc agtgccgcca
     7021 gcgtgcaggc gaaattgccc gccagcatga atccgagaat cgccaccgca ggccattgct
     7081 cccggcgggg aagcaatggc gctctgaacc tcatgatcac aggaatcatg cacagcgcag
     7141 ccagcgtgaa acgcagcgtg gcgaactgca acggccccgc atcggctagt ccggttttca
     7201 tcgccaccca gttgtagccc cagatcaggg acagcagcgc gagtgctacg ccggcgttac
     7261 gccgctcggg atcgagatcg gacatgggat tatcggtgcc ggcggcgcat cgccggatgt
     7321 ttgcgtttgt cgagcacgca tcctagcgtg gctccggtgt cttgcgacga tggcgccgga
     7381 aaattccact atacttgccc ggcattcggc cgcccggtcc agtggcaggc cggccatgcc
     7441 aaaaagaaaa cacattggga gggggcagct atgatttggt ctcaggtcta cgacccgttc
     7501 ggcaatacca tcctttctac gatcgtctgc gcgatccccg tggtagtgct gctcggcggg
     7561 ctcggaatct ttcaaatccg cgcccactat gcggcgattg ccgggttagt cgcggccgcg
     7621 ctgatcgcga tgttcgtcat aggcatgccc ggcggtttga cgttgcgtgc cggcttttat
     7681 ggtgcgctgt ttgccgttac cacgatctgc tggatcattt tcaacctgat cttcctgtac
     7741 cgcatgacgc tcaaggcggg cctgttcaag atcctgcagg acagcatcgg cgccgtcacc
     7801 aacgacaggc gcctgcagct tgtgctgatc gcattctgct tcggcgcctt ctttgagggc
     7861 gcggccggtg gcggcacgcc ggtagcggtg accggcgcaa tcctgatcgg ccttggcttc
     7921 aaccccctgg cggcgtccgg cctgtcgttg atcgccaata ccgcgccggt ggcctacggc
     7981 gggctgggca cgcccatcat cgtgctgcat tcggtgaccc agcccgacaa tgcggactac
     8041 ctgctgaccc tttcggcgat ggtgggacgc cagttgccgt tcttttcggt catcgtgccg
     8101 gtctggctgg tcctcacttt ctgtggatgg cgccgcacgc ttgaggtctg gccggcaatc
     8161 ctggtggctg gcgtgtcgtt cgcaatttcg cagttcctga tttcgaacta ccacggcccg
     8221 tggctggtgg acatgctcgg ggcgatgatc tcgatgttga tcctggccgt cttcctggcc
     8281 tactggaagc cgaagaacgt gatgctggac gcatccggcg gcggcgtgac cgtccctgcc
     8341 ggagaagcgg ccaaggtaca ccatggccac ggccgcgaca tggtgatcaa ggcgtggacg
     8401 ccgtgggtga tcctcaccgt catcctgttc ctgtggggtc tacccatcgt caaggcgttt
     8461 ctcaacgtga agggcatcac ttactgggag tggcagattc ccggcttgca taacgtggtg
     8521 cagcgcgtgg aacctgcggt cgccgtcccc cgtgtcgaag gagccgtctg gaagtgggga
     8581 atcatctcgg cgaccggaac ggggattctg ctggcggcga tcatcggcgg cttggtcatg
     8641 ggtttcaagc cgcgcgagat gttcaggacc tggatcgaaa ccttcgggat catgaagtac
     8701 tccctgctga cgatcctggc gatgcttgcg ctgggctaca cgacacgcta ctcgggcatg
     8761 gacggcacgc tcgggcttgc gttcgccaac accggctggc tctacccctt cttcggcacg
     8821 atgatcggct ggatcggcgt cgcgttgacg ggtacggatg ccgcgtcgaa cgcactgttc
     8881 ggcagccagc aggtgatcac ggcgaacaag ctgggattga atccgaccct gatggccgcg
     8941 gccaacagtt cgggcggggt gatgggcaag atgatcgatg cccagagcat agtggtcgcg
     9001 tcggtggcga ccaaccagca aggcagggaa ggcgtcatcc tgcgcttcgt gttctggcac
     9061 agcattgcgc tggccgcatt ggtcggtgtg ctggtcatgc tgcaggctta cgtgccgttc
     9121 ttccaggcca tggtcctgaa ataacccgtg ccacggagcc acgaattggc acggcagcct
     9181 tggctgccgt gccattttgt tatgaggaga agcagaatgc gcaggcgctt tgccgaagcg
     9241 aaagcgccca agatggacca accgccagca aaggacgggg ttttcaagat cgcggatggc
     9301 cgcgcgggaa gataagggga ggaagatgat cgaacagaaa ctcgccagcc tcggaatcgt
     9361 gctgcccgcg gctgcggttc cggcgtttca gtatgttgcg gtcaccgtcc acgagcgact
     9421 ggcattcgtc agcggtcaac tgccgcgcga aggcgacgag gtgcgtatcg tcggcaaggt
     9481 cggtggtgag gtcggcgtcg agcgcgcaca ggaggcggcg cggctgtgca ttctgcaagg
     9541 actcgcctgc ctgaagcagg cgctgggctc gctcgaccgc atcgaacgca tcctcaaagt
     9601 caccggattc gttgcctcgg cgcccatgtt caaccagcag ccgaaagtga tcgacgcagc
     9661 ctcggaactg ctggtgcagg ttttcggaga aagcggcagg cacgcgcgca gcgcgatcgg
     9721 ggtcgcggag ttgccgcgca acgcctgcgt cgaaatcgag ataatcgtgg ccttgcggcc
     9781 ctgatcgatg ttgcgcgcct cgatggtgtt accatcgcga gatgaaaagc gggcgcctgc
     9841 cggcaccggt ccctcatcgg gatgcggcgc ggcaggcgca tttcatgtaa gcagccgata
     9901 acccgaccgc aaagtcatgc ctacctcaga gaacatcctg gcgatactgc gatcggccgg
     9961 gatcgtcgag aacagccaat ccgaagagac ttatcgcgag gttcacgccc aacagatcgc
    10021 cctgctgttc cgtcagcttc ctgcggcact cacggcgacg ctgctgatag cgttcatcgt
    10081 ggccggtgtg acctggcctg tcgcggacgc gaacctgctg ttctcctggc tcattgccgt
    10141 gtgtgcgctc actgggcacc ggttcttgct atggcgtcgt tttgcggcaa acgcgcctcg
    10201 ggccgagcag atggaacgct ggtcgcggag cttttttgtc ggcaccctgc tgtccggcat
    10261 ggtctggggc gcgggcagcg ttctgctggc aggagaggac ctcattcacc agttcttttt
    10321 cgggctgctg ctcgccggtc tggctgcggg gggcgtttcc acgctgtctt cttatcgcgg
    10381 cgtttacgcc gcattcctct tgccgagctt ggtgccgctc gccgtgcgca tggcgttgta
    10441 cctggatccg gtacacgtcg cgatggcctc gatgatgttg ctgttcgcag cgctgatgct
    10501 ggggatttcc gcgcgcattc acgccaccat cgccgagtcg ctgcggctgc gttttcgcaa
    10561 cctcggcctg gtggatacgc tttccgccac caagggcaac ctggaacagg caaacacggc
    10621 cctgcagcgc gagatcggcg agcatcgcaa ggcgcaggag atcctgatga agtcggagca
    10681 gaagctgcgt ctgcacgccc accaggcgcc gcttgccttc atcgaatggg acctgtcgtt
    10741 tcgcgtggtc gagtggaacc ccgcggccga gcgcatcttt ggctacagcc ggcaggaggc
    10801 gctgggacgg cacgccgcca gcctggtcgc cgccgagcgc agttgcgaca ccatcgccgc
    10861 gctgtggaag cggctgctta tcgacaagca cagcgcgcag gtgacgatag acaaccgcac
    10921 cagggaccaa cgcaacatcg tttgcgagtg gtactacacg ccgctggtgg atggacaagg
    10981 caaggtgttg tcagtgatga ccctggcgca ggacgtgacc gccaaccgcc aggcgcagga
    11041 acggctcaac tacctcgcct atcacgacga gctcaccggc ctgcccaatc gctcgctata
    11101 taacgaccgt ctgtcgcagg cgatgatcga ggcgcgccgg caggggcgtt atgtcggcgt
    11161 gatgctgctc gacatcgacc atttcaagat ggtcaacgac accttgggcc atgaggccgg
    11221 ggacgagctg ttgcgagact tcggcaagcg cttgtctgtg tgcgtgcgcg agagcgatac
    11281 cgtggcgcgc ttcggcggcg acgaatttgc gttggtgctg gccgacatgg ccgatccgcg
    11341 tgacgcgata ttcgtggcgc aaaagatgct cgacagcttc gctccgcctt ttttcgtcgg
    11401 cgggcgcgag ttattcgtcg gccccagcat cggcatcacc atttatcctt tggacagcga
    11461 cgacctggac gggctcgtaa agaacgcgga ttcggccttg tatcacgcca aggcgcaagg
    11521 gcgaaacaac ttccagttct attccgccga actgacggcg cgtgcgcagt cgcgccttga
    11581 cacggaaacc agtctgcgac gggcgctgga gcgacgcgaa tttctgctgc atttccagcc
    11641 caagttcagc ctggaaaccg gtgagatgac cggcgtggaa gcgctgattc gctggcagca
    11701 tcccgagctc ggcatggtgt cgccgcagga cttcatcggc attgccgagg aaagcggatt
    11761 gattctgccg atcggcgaat gggtgttgcg tgcggcctgc cagcaggtga agcgctggca
    11821 cgatgaagga ttggcgcagg tcaggctggc ggtgaatctg tcgaccaagc agttccgtca
    11881 cagccggttg aaggaaatgc tggccggcgt cctcgcagaa accggattcg atccgcgctt
    11941 cctcgagtta gagattaccg aaagcatgtt gatggaaaac agtgccgcgg tgagcgaagt
    12001 gctggacgac ctcaaggcga tgggtatctc gatctcggtg gacgacttcg gtaccggcta
    12061 ctcttcgctt tcgtatctca agcgttttcc catcgacgca ctgaagatcg accggtcctt
    12121 cgttcgtgac gtccctgcgg atcacgacga tgcagcaatc gtgcgcgcga ttatcgccat
    12181 gtcgcgcagc ttgcgcatga aggtcatcgc cgagggcgtg gaaacccagg aacagcaaca
    12241 attcctgcgc gccgaaggct gcgacgaaat ccagggtttc ttatcgggtc gcccgatgcc
    12301 gcccgatgaa ctcgtgcaaa ggctgcgttc ccggccggct tctgactgat cggcggcggc
    12361 aagcgcttcg tcgcgagccg ggcttccatt taagatacgc agcctgcgcg acacgggccg
    12421 acgcgttgat gcgctcgctc cggcggcgcg gattcagacg ccggtcaccg cgttggggga
    12481 aggggcaatg aaatacaaca gactgggatc gagcgatctc aacgtatcgg agatctgcct
    12541 cggcaccatg acttggggcg agcagaacag cgaaaaagaa gcgcatcagc agctccacta
    12601 cgccgtctcc cgcggcatca atttcatcga cgcggcggag atgtacccgg tgccgcccaa
    12661 ggcggaaacg caaggccgca cagagtctta cctcggtact tggctcagaa ggcagcaacg
    12721 cgacaaagtg atcgttgcca ccaagatcac cgccccaggc cggggattca attgggtgcg
    12781 cggcgggccg aaggcggtga acaggaaaag cattcaggag gcgctgaacg gtagcctcaa
    12841 gcgtctgcag accgactatg tggacctgta ccagatccac tggccggacc gctatctgcc
    12901 gctgtttggc aacattttct acgacccgaa gcaggaacgc ccgactgtac cgatcgaaga
    12961 tcagctcgag gccattgccg agttcatcaa ggccggcaag atccgttacc tcggattgtc
    13021 caacgagtca ccttggggcg cactggagtt catccgccac gcgcggcaga aggggctgcc
    13081 gatggtggcc tcgacccaga acgcctacaa cctgctcaac cgtacttttg aaaccggtct
    13141 gtcggaggtg acgcggcgcg agggcgtgcc gctgcttgca tacagcccgc tcgcctttgg
    13201 ccacttgtct ggcaagtatc tcaatggcgc gaagccggaa ggggcgcgaa tgaccaggta
    13261 cccgccgttc ggccagcgtt acgacaagcc caacgtaatg ccggcggtcg ccgcctatgc
    13321 cgaactggcc agacgcgccg gcctgtcggc ggcagccctg gcgatcgggt tcgtacgcag
    13381 ccgctggttc tgcgccagcg tcatcatcgg cgcgactact ccggcccaac tacgcgaaaa
    13441 catcgatacc gaaaacctga gcctcgggga cgccgtcctt gccgatatcg acgctgttca
    13501 cctgcgctat cccaacccgg ccgtgtgacc gcgctaggcg ccgatcccgg agcaatccgg
    13561 ggtgcgcggc aagcgcgtct gaatgccgcc ggatgtttga catcccttag attattatcg
    13621 ataatccgcc gtttcgttcg gaggggttcc cgagcggtca aagggatcag actgtaaatc
    13681 tgacggctct gccttcgaag gttcgaatcc ttccccctcc accaagcatt gagagacggg
    13741 gtcggttcgg atcggtggcg ggatcgtcaa agcttcttct gaggtaccaa cggcaagcag
    13801 tttcggtgtt tgagcccgga ttcaggcggg tgtagctcaa tggtagagca gaagccttcc
    13861 aagcttacga cgagggttcg attcccttca cccgctccag aaagaaatta gcgaggcagt
    13921 cgctgcgact tggttaaagc ggggcgcgaa caaacggcac cggcgttccg aaccgcccat
    13981 gtagctcagt ggtagagcac tcccttggta agggagaggt caccagttca atcctggtca
    14041 tgggctccaa aggattcagg gttgcacagg gttcaggggt cactacttaa aaaagctaag
    14101 acgaggacag cgaga
//
LOCUS       DASQOP010000361         3895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_997001, whole genome shotgun sequence.
ACCESSION   DASQOP010000361 DASQOP010000000
VERSION     DASQOP010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997001"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VE030_14030"
     CDS             <1..364
                     /locus_tag="VE030_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013689136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZF24571.1"
                     /translation="RGFTPVTSRMLPLLLHPSRVKDAQLTKTVRDMAERTGIEAYLRQ
                     QRAIISRPDFRPGLPKINCPALVLCGRQDQLTPLECSEEMAAAIPGAKLAVVEECGHL
                     STLERPQIVNTALREWLA"
     gene            680..1702
                     /locus_tag="VE030_14035"
     CDS             680..1702
                     /locus_tag="VE030_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HZF24572.1"
                     /translation="MTARTLGALALCIAAACGSALAQDKPVELKFSHWLPPAHPLHKT
                     AQAWAKSIEQASGGTIRIAIYPAQQLGKAIDHYDMTVKGIADVSYINVGYQAGRMLVA
                     NAAQLPFMMDNADNGSRAYDVWYRPYAQKDMPGVKLCLMFVHDPGTFHSRTRVSSPEQ
                     IKGLKVRPAQAVVADWVKLLGASTVNVSAPEAREALERGVADAITFPWESIYLFGINK
                     VIRFHIDARMYTTGFAWILNPDRYNSLSPAQRKVIDGHCSSDWAYRVGKEWGDFEAAG
                     REKTKADNAQTVVPLTPPELAAWKKSAEPVVGIWSDEVRKQGYDPAKLLAEFDATLLK
                     YGAKAK"
     gene            1723..2184
                     /locus_tag="VE030_14040"
     CDS             1723..2184
                     /locus_tag="VE030_14040"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HZF24573.1"
                     /translation="MERFVRMVEGTAGLLLLTVALATFATVILRKLFNYSPPDWFDLA
                     QLLLGISIFWGIASACYRNGHILVDLLWEFLSSRGRRILDITATSILLVFMAALSLML
                     ADAIRDTAAGNVLTMELKLPVWPFHVLAGAGILAGTILTAVRLVRLCSRGE"
     gene            2192..3496
                     /locus_tag="VE030_14045"
     CDS             2192..3496
                     /locus_tag="VE030_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HZF24574.1"
                     /translation="MIDRDLIAVLGFALMFALMLLRVPIGVAMGIAGVLGYGVVSGWG
                     PALALLGKVPLSTVTDYKLAVIPMFILMGSFAAAAGMSRELFNAGSAWFGHLRGGLAV
                     GSIAACGGFAAINGSSVATVATMTNVALPEMRRAGYEPGFAAGVIAAGGTLGIMIPPS
                     VIFVLYGIMTETDISQLFAAGVLPGLLAMGMYAVTIQIVGRLRPQHMPRGARHGWHER
                     FSALREIWSTAALFLLVLGGIYGGLFLVDEAAAVGAIGALVIGLARGRLSRRAIMECL
                     VSSVRVSASIMVIVIGAYLFGYFLTITQATQQIVEFMVHLPLGAYGVLALIMIGYLIL
                     GAIMDELAMILLTVPIVFPVIMQLGFDPVWFGVIIVMAVTLGLVCPPVGMNVFVLNSI
                     ARDISLPRIYAGTMPFIATDLVRLVVLCAFPVISLWLPRTMA"
BASE COUNT          676 a         1206 c         1251 g          762 t
ORIGIN      
        1 acgcggcttc acgccggtaa ccagccgcat gctgccgctg ctgcttcacc cctcgcgggt
       61 caaagatgcg cagctcacca agaccgtccg agacatggcg gaacgcaccg gaatcgaagc
      121 ttacctgcgg cagcagcggg cgatcatctc ccggcccgat ttcaggcccg ggttgccgaa
      181 gatcaattgt ccggcgctgg tgctgtgcgg gcgccaggac caactcacgc ccctggaatg
      241 cagcgaggaa atggccgcgg cgatcccggg ggcgaagctg gcggtggtcg aggagtgcgg
      301 tcacctgtcc acgctggagc ggccacagat cgtgaacacg gcacttcgcg aatggctggc
      361 ctgacctaga caagcactta ccacggagga cgcggaagac acggaggaaa accagaggcg
      421 aggaaaaaca cgtgggagtt aaaagcaggc ccggtgccga agtgacctac agtagcgggg
      481 attaactctc gaaggttctt tttccgtcct tgcctttcct ccgtgtcctc cgcgtcctcc
      541 gtggtgagcg ctttgttcca gtgcccgatc gagaatggaa atggcgattc gttcctgcgt
      601 ctatggcggc ccgctatact tcgcgctggc ggtcaaaaca acaaatgcgc cgcacaacag
      661 acccgcggag gagatcagcg tgactgcacg aaccttgggc gccctggcgc tctgcattgc
      721 cgcagcctgc ggaagcgctc ttgcccagga caagccggtc gagctgaagt tttcccactg
      781 gttgccgccc gcgcacccgt tgcacaagac cgcgcaggcc tgggccaagt cgatcgagca
      841 ggcatcgggc ggcacgatca ggatcgcgat ctatccggcc cagcaactcg gcaaggcgat
      901 cgaccattac gatatgacgg tcaagggcat cgccgacgtg tcgtatatca atgtcggcta
      961 ccaggccgga cgcatgctgg tggcgaacgc ggcccaactc ccgttcatga tggacaatgc
     1021 cgacaacggg tcgcgcgcct acgacgtctg gtaccgcccg tacgcgcaga aagacatgcc
     1081 gggcgtaaag ctgtgcctga tgttcgtaca cgatcccggc actttccact ccagaacacg
     1141 ggtcagttcg ccggagcaga tcaagggtct gaaagtgcgg ccggcgcaag cggtggttgc
     1201 cgactgggtg aagctgctcg gcgcatcgac ggtcaacgtc agtgctcccg aggcgcgcga
     1261 ggcgctggag cgcggtgtcg ccgacgccat cacctttccc tgggagtcca tttatctgtt
     1321 cggcatcaac aaggtgattc gtttccacat cgacgcgcgg atgtacacca ccggcttcgc
     1381 gtggattctg aatcccgacc gctacaattc actgtcgccc gcacagcgca aggtcattga
     1441 cgggcattgc agttccgact gggcctaccg ggtcggcaag gagtggggcg acttcgaggc
     1501 agccggacgg gagaagacga aagcggacaa tgcgcagaca gtcgtgccgc tcacgcctcc
     1561 agaactggcg gcctggaaga aatcggccga gccggtggtc gggatctgga gcgacgaggt
     1621 gcgcaagcag ggctacgacc cggccaaact cctggcggag ttcgacgcaa cgctcctgaa
     1681 atacggggcg aaggcgaagt agcggcgccg gcgggatccg caatggagcg ctttgtccgc
     1741 atggtggagg gaacggcggg cctgcttttg ctgaccgtcg cgctggccac tttcgcaacg
     1801 gtcatcctgc gcaagctctt caattactcg cctccggact ggttcgatct ggcgcaattg
     1861 ctgctgggca tcagcatctt ctggggcatc gcctccgcct gttaccgcaa cggccacatc
     1921 ctggtggatc tgctctggga gttcctgtcc tcgcgcggac gccgtatttt ggacatcacc
     1981 gcgacctcga tcctgctggt gttcatggcg gcgctctcgc tgatgctcgc cgacgccatc
     2041 cgcgacacgg ccgcaggcaa cgtgctgacg atggagctga agctgccggt gtggcctttc
     2101 cacgtgctgg cgggtgccgg cattctggcc ggcacgatcc tcaccgctgt gcgcctggtc
     2161 cgcctctgca gccgcggaga atgacccggc gatgatcgac agggatctca ttgccgtgct
     2221 cggcttcgca ctgatgttcg cgctgatgtt gctgcgcgtt ccgatcggcg ttgcaatggg
     2281 cattgccggc gtgcttggtt acggcgtggt gtcgggttgg ggcccggccc tcgcgctgct
     2341 gggcaaggtg ccgctgtcca ccgtcaccga ctacaagctc gcggtgatcc cgatgttcat
     2401 cctgatgggc tcgtttgccg ccgcggccgg aatgagccgc gagctgttca acgccggtag
     2461 cgcgtggttc gggcatttgc gcggcggcct ggcggtgggc tcgatcgccg cctgcggggg
     2521 attcgccgcg atcaacggct cgtcggtggc cacagtcgcg acgatgacca acgtcgcgct
     2581 gccggagatg cgccgcgccg gctatgagcc cggctttgcg gccggcgtga tcgctgccgg
     2641 cggcacgctc ggcatcatga tcccgccctc ggtgattttc gtgctctacg gcatcatgac
     2701 cgagaccgac atctcgcaat tgttcgcggc gggcgtactg cccggcctgc tggcgatggg
     2761 gatgtacgcg gtcaccatcc agatcgtcgg ccgcctgcgc ccgcaacaca tgccgcgcgg
     2821 cgcccgccac ggctggcatg agcggttcag cgcactgagg gaaatctggt cgaccgccgc
     2881 gcttttcctg ctcgtactcg gcggcatcta cggcggattg tttctggtgg acgaggcggc
     2941 cgcggtcggc gcaatcgggg cgctggtcat aggactggcg cgcggccggc tgtcacgccg
     3001 ggccatcatg gaatgcttgg tcagttcggt gcgcgtgtcg gcttcgatca tggtgatcgt
     3061 gatcggcgcc tatttgttcg gctatttcct caccatcacg caggccacgc agcagatcgt
     3121 cgagttcatg gtgcacctgc cgctcggcgc ctacggcgtg ctcgcgctga tcatgatcgg
     3181 ctatctgatt ctcggcgcga tcatggacga gctggcaatg atcctgctta ccgtgccgat
     3241 cgtgtttccg gtgatcatgc aactgggttt cgacccggtg tggttcggcg tgatcatcgt
     3301 gatggcggtg acgctcggcc tcgtctgccc gccggtgggc atgaacgtgt tcgtgctcaa
     3361 ctcgatcgcg cgcgacatca gcctgccgcg catctacgcc ggcaccatgc cgttcatcgc
     3421 cacggacctc gtccggctgg tggtgctttg cgcgtttccc gtaatttccc tgtggctacc
     3481 ccggaccatg gcatagcagg taagctctta acgcagaggg cgcggagaac gcagaggaaa
     3541 aacaaaaacg agggaaagca acttagagat aaaggcgggg tgactgatcg cgataatctg
     3601 ccgctaactc tcgccgcgaa ccatcatgag ccaatgaaag gttttcatcc aaacctattt
     3661 tcttgttctt gcctttcctc cgcgtcctct gcggtgaggg tttagggtgc ctagtcccag
     3721 aagctcagag ttgagctgac aggcagtgtt ggcaagcgga ccccaaacca tattgggagc
     3781 ggctggaagc gtccaggaac cgtcattcac ggctgccgcg ggtacccccg ctttcctttg
     3841 gtactcgacg tttcagcggt acttctccgc gcagcgaatt atggcggctc tgttg
//
LOCUS       DASQOP010000362         8697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2001122, whole genome shotgun sequence.
ACCESSION   DASQOP010000362 DASQOP010000000
VERSION     DASQOP010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8697
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2001122"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1246
                     /locus_tag="VE030_14050"
     CDS             <1..1246
                     /locus_tag="VE030_14050"
                     /inference="COORDINATES: protein motif:HMM:NF017775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HZF24575.1"
                     /translation="FVGILWALSGPFAIPYNGAEIVIPGYMVWAALVYAIVGTWMTHK
                     IGKALIGLNFNQQRFEADFRFGLVRFRENTEGVALYGGEQDELRNFRSRFANVVNNWW
                     LIMRRQKILNSFTIGYNQLAVIFPFVVAGNRYFAGTIQLGGLMQISNAFGQVQGSLSW
                     FIGAYNSFAAWKATADRLLGFHYAIEKAREDMRQQSGVRQAQDGNEDLVIDNVALSLP
                     NGQPLISTSNATIKSGESLLICGPSGSGKSTLFRAIAGIWPFGKGRMRMPAGFRVLFL
                     PQRPYLPIGTIRDVVSYPASPTEFSDEQIKGVLDAVGLPQLVDRIDDVQHWSLQLSPG
                     EQQRIAFARALLQKPAWLFLDEATSALDDSAEEKLYRMLKESLPGTTIISVGHRAALI
                     AFHSRRLELREDGDGTRVLAPI"
     gene            complement(1429..2073)
                     /locus_tag="VE030_14055"
     CDS             complement(1429..2073)
                     /locus_tag="VE030_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24576.1"
                     /translation="MRWVFLVLLAVNIGFGVFVYLRDKAPNPDAQLIALQMNADKVRI
                     VPPRPAPAPAPVTASARVACAEWGGFGASELARAQNALDRLALGARARRVEVSVTAGY
                     WVFIPPQRSQAAMERKLTELKELRVSEYFPLQDPGRWRYAISLGVFRSEEGAARYLAQ
                     LRQKGVRSATVGEREQRVTQTAFQISAPTEEESAKFAELKNEFPGTELRAVECH"
     gene            complement(2070..2798)
                     /locus_tag="VE030_14060"
     CDS             complement(2070..2798)
                     /locus_tag="VE030_14060"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HZF24577.1"
                     /translation="MILAVDAGNSRIKWALHDRNAFVHEGRVARADLALLERAWAHLP
                     AADTIVVANVAGEAVRAGLQKLFVRWKAEPRWVAATGAQCGVVSRYDDPSQLGADRWA
                     ALIGARQLVSGSCLVVNAGTAMTVDALSTQGEFLGGIIVPGFDLMHEALAANTAGLSS
                     ERGSFASFPRSTRDAITSGAIQALCGAVERVRAAMVADGHGEPTLLIGGGAAELMAQR
                     LGRPARIADKLVLEGLVRIAQERR"
     gene            complement(2838..3809)
                     /locus_tag="VE030_14065"
     CDS             complement(2838..3809)
                     /locus_tag="VE030_14065"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HZF24578.1"
                     /translation="MTPHTLEVLRLLQNGEFRSGEAIGKALGITRGSVSNALANIDAF
                     GLTVHKVHGRGYRLVTPVQWLSRDQILAQFGGQARFDIEVVDETGSTNTDLLERAAQG
                     APSGTVLVAELQTHGRGRRGRNWHSGLGGALTFSVLWRFEQGAGFLSGLSLAVGIALA
                     RVMRRHGAEEVMLKWPNDVLWRHLKLAGILIELAGDVMGPTVAVIGVGINLRLPEAVK
                     ARIDQPVVDLAKIGIDMDRNRLFAEILAELDMVLRKFSGKGFAPFRHEWDKMHAYRDR
                     MVRLRMPDKTELEGKVEAVAEDGALMIKTRSGTRKFYGGELSLRPAA"
     gene            3893..4897
                     /locus_tag="VE030_14070"
     CDS             3893..4897
                     /locus_tag="VE030_14070"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZF24579.1"
                     /translation="MSRPQLGHIRRACHLCTAALLLVFFAACDKERAYPARPVNLIVP
                     FPAGGSSDLIARLVSERAGRALGQQIVIENRPGAGGNIGTEAAARAAPDGYTLIQCTI
                     GTCAINLAIYHDLPYDLERDFAPIVLFGSMANVLTVNPAVKANSVAELVALARKQSSK
                     LTFGSSGYGSSPHLSGELFKQAAGIDILHVPYRGSAPAITDLRGGQIDMFFDNTPSIL
                     PHIKAGALRALAITGAQRSPRLPDVPTMQEAGFRDFVIAPWFGVMAQQKTPARIIERL
                     NKAFNEALSDPAVLRQFADMDVKAGGGTPEEFRAHIRAETRRWGDLVRARGIRAEELR
                     "
     gene            4912..6117
                     /locus_tag="VE030_14075"
     CDS             4912..6117
                     /locus_tag="VE030_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HZF24580.1"
                     /translation="MKITAIETLRLPERPNLLLAQIHTDEGLVGLGETSRGAQAVEAQ
                     LHDLVAPYLLGKDPREIQKHSKHLMGSYLGFASSSTEIRAASVVDIALWDILGQALNQ
                     PIHTLLGGLTRDRIRAYNTCAGYNYNTRSVVRRSLRVGEPAPKSEGRYDDQIAFVHRA
                     DELAQSLLEEGYTAMKIWPFDPYAEDNGGGFIPERDLDIALEPWRKIRRAVGDTIEVM
                     AEFHSLWNLQQAKQIARALEPYRPYWSEDPVRMCDVDTLAEYARSTTIPVCASETLGG
                     MYPFRDVLANQAADIVMLDIGWCGGLTEARKIAALAEAWQRPVAPHDCNGPVVWVASI
                     HFMAHIPNALIMEVVRAYFSTWYKDILTELPLVKDGYIRPLNGPGLGTRLLPDLVTRQ
                     NSIVRRSGA"
     gene            6272..8398
                     /locus_tag="VE030_14080"
     CDS             6272..8398
                     /locus_tag="VE030_14080"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZF24581.1"
                     /translation="MRWLAICLILLITALTSMAPVSATPVQRPHIEAELIPEKSSLRP
                     GAPLTVALRLKMENRWHTYWKNPGDSGLPTRISWTLPQGFSAGPVQWPAPRRINVGPL
                     TNFGYEGGIFLLTDIQVPAGLAAGTAVPITAKADWLVCEEICIPGDATFSFMLPVSNE
                     PAAADPKWAKAIERTRQQLPRPLEGWNLDAYLRGDDLVLRLSSGRARVVRIHNLTLFP
                     DQDGWIENASPQRMIRTTGGYELRVASAPAIRSKSGPLTGLIVAEPGLAQGVAAAVVE
                     VPVESASALADEILEQPALSPFADVGLLAALGLAFLGGVILNLMPCVFPVVSIKVLSF
                     VEQARGNPARLRAHGLAFAAGVLVSFWVVAGMLLALRAQGEALGWGYQLQSPSVVAAL
                     ALLFFVLALNLSGLFEVGTSLQSLAAKLRLRNDYADSALSGLLATLVATPCTAPFMGA
                     ALGFALTQPASEAMLVFSALALGMSSPYLLLCFWPALIRRIPRPGRWMETLKQVLAFP
                     LFLTVVWLAWVLGKQAGVDAVARLLAGLTLIAAALWMIGRWGASTETGKRAATVVALA
                     LAVAGCVFAWPGNKTADAVAEEVTGPWKRWSPDALASARAEGRAVFVDFTAAWCVTCQ
                     VNKRLVLESDALHARFKERNVVRLRADWTNQDAQITAALRDLGRIGVPVYAVYAAGSK
                     GAPILLPELLTRERVLRAIDVAAPPS"
     gene            8436..>8697
                     /locus_tag="VE030_14085"
     CDS             8436..>8697
                     /locus_tag="VE030_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HZF24582.1"
                     /translation="MSKLVATFLAVSMAALVSAAQAAVAPGQAAPEFSLVDINGRVQK
                     LSDYRGKYVVLEWFNSECPFVQKHYESGNMQALQARYTQKGVA"
BASE COUNT         1554 a         2797 c         2754 g         1592 t
ORIGIN      
        1 cttcgtcggc atattgtggg cgctgtccgg accgttcgcg atcccctata acggcgccga
       61 gatcgttatc ccgggttaca tggtgtgggc ggcgctggtc tacgcgatcg tcggcacctg
      121 gatgacccac aagatcggca aggcgctgat cgggctcaac ttcaaccagc agcgcttcga
      181 ggccgacttc cgtttcgggc tggtgcgctt ccgggaaaac accgaaggcg tggcgctata
      241 cggcggcgaa caggacgaac tgcgcaattt ccgctcgcgc ttcgccaacg tggtcaacaa
      301 ctggtggctc atcatgaggc gccagaagat tctcaactct ttcaccatcg gctataacca
      361 gctggcggtg atattcccgt tcgtcgttgc cggcaaccgc tatttcgccg gcaccatcca
      421 gctcggcggg ctgatgcaga tttcgaacgc ctttggacag gtacagggtt cgctgtcgtg
      481 gttcatcggc gcctataaca gcttcgccgc ctggaaagcc acggcagacc gcctgctcgg
      541 cttccactat gcgatcgaga aagctcgcga agacatgcgg cagcagtccg gggtgcgaca
      601 ggcgcaggat ggcaacgaag atcttgtcat cgacaacgtt gccctcagcc tgcccaacgg
      661 ccagccgctg atttcaactt cgaacgcgac catcaagtcg ggggaaagcc tgctgatctg
      721 cgggccttcc gggtccggaa agagcacgct gttccgggcg atagccggga tctggccatt
      781 tggaaaagga cgaatgcgca tgccggcggg cttccgcgtg ctgttcctgc cgcagcggcc
      841 ctacctgccg atcggcacga ttcgcgacgt cgtaagctac cccgcgtcgc ctaccgagtt
      901 ctccgacgag cagatcaaag gggtgctcga cgcggtcggg ctgccgcaac tcgtggaccg
      961 gatcgacgat gtccagcact ggtcactgca actctcgccc ggtgaacaac agcgcatcgc
     1021 ctttgcgcgc gcgctgctgc aaaagccggc gtggttgttc ctcgacgagg cgacttccgc
     1081 gttggacgat tccgcggaag aaaaactcta ccggatgctg aaggaaagtc tgcccggcac
     1141 caccatcatc agcgtcggcc accgggcggc gctgatcgca ttccactcgc gtcggctgga
     1201 actgagggaa gacggcgacg gcacccgcgt gctggccccg atctagaacc accaaagacc
     1261 aattcaaagg cggcgacgca agggcgcaaa ggagaaagga aagtacgcaa agggatgcct
     1321 gactgattac gtacgagcaa accgcgatca ttcagggttc tcgttggtgt tgaggatctt
     1381 tggctcttct ccgtggtctc ggtgccctct gcggttcggg aatcggactc agtggcattc
     1441 gacggcgcgc aattcggtac ccgggaattc gtttttcagt tccgcaaatt tcgccgattc
     1501 ctcttcggtc ggggcgctga tctggaatgc agtctgggtc acgcgctgtt cacgttcgcc
     1561 gaccgtcgcc gaacgaacgc ccttttgtcg cagctgcgca aggtacctgg ccgcgccctc
     1621 ctcactgcgg aacacaccca gtgaaatcgc atagcgccag cggcccgggt cctgaagtgg
     1681 aaagtattcg ctgacccgca gttctttgag ctcggtgagc ttgcgctcca tcgccgcctg
     1741 cgatcgctgc ggcggaatga atacccagta gcccgcggtc acgctgacct cgacgcggcg
     1801 cgcgcgtgct ccgagcgcaa gacgatccag ggcgttctgc gcgcgcgcca gttcgctcgc
     1861 gccaaatccg ccccattcgg cgcaggctac gcgcgccgat gccgtcaccg gcgcaggcgc
     1921 aggcgccggc cgcggcggca cgatgcgcac cttgtctgca ttcatctgca gcgcgatgag
     1981 ctgcgcatcc ggattcggcg ccttgtctct gaggtaaacg aaaacgccga acccgatgtt
     2041 gacggcaagc agcaccagaa agacccacct catcgccgtt cctgcgcgat ccgcaccagg
     2101 ccttcgagca ccagcttgtc cgcgatcctg gccggcctgc cgagccgctg cgccatcagc
     2161 tccgccgcgc cgcctccgat caacagggtc ggttccccgt gaccgtccgc caccatcgct
     2221 gcgcgcacgc gttccaccgc gccgcacagc gcctgtatcg caccgctggt gatggcatcg
     2281 cgcgtgctgc gcgggaacga ggcaaagctg ccgcgttcgg aggacaggcc tgccgtgttc
     2341 gcagccaacg cttcgtgcat cagatcgaag ccgggaacga tgataccgcc gaggaactcg
     2401 ccctgcgtgc taagagcgtc caccgtcatc gcggtgcccg cgttaaccac cagacaggaa
     2461 ccggaaacca gttgccgcgc gccgatcagt gccgcccagc ggtccgcgcc aagttgcgac
     2521 ggatcgtcgt agcggctgac aacaccgcat tgggctccgg ttgcggcgac ccatcgaggt
     2581 tcggccttcc agcggacaaa taatttctgc aaacctgcgc gcaccgcctc gccagccacg
     2641 ttggccacga cgattgtatc cgcggcaggc agatgtgccc acgcccgctc gagcagggcg
     2701 aggtcggcac gcgccaccct tccttcatgg acgaaggcgt tgcggtcatg gagcgcccat
     2761 ttgattcggc tgttgccggc atcgacggca aggatcacgg ttcgagagac tttcgggttt
     2821 ggataggaat ctcgtactta cgcggcaggt cgcagactca gctctccgcc gtagaacttg
     2881 cgcgtaccgg agcgggtctt gatcatcagc gcaccgtcct cggcgaccgc ttcgaccttg
     2941 ccttcgagtt cggtcttgtc cggcatgcgc agacgcacca ttctatctcg gtacgcgtgc
     3001 atcttgtccc attcatggcg aaacggcgca aaacccttgc cggagaattt cctcagaacc
     3061 atatcgagtt cggcaagaat ctccgcaaac agccggttgc ggtccatgtc tatgccgatc
     3121 tttgccaggt ccaccaccgg ctggtcaatg cgtgccttca ccgcctccgg caggcgcaga
     3181 ttgattccca cgccgatcac cgccacggtg ggtcccatta cgtcgccggc aagttcgatc
     3241 aggatgccgg caagcttgag gtgacgccac agcacgtcat tcggccattt cagcatcacc
     3301 tcttctgctc catgccttct catcactctt gcaagcgcga taccgaccgc gagactgagc
     3361 ccggaaagaa accccgcccc ctgttcgaaa cgccagagga ccgaaaacgt gagtgcgccc
     3421 cccagtccgg aatgccagtt gcgcccgcgc cgtccgcgcc catgggtctg caattcggcc
     3481 acgagcacgg tacccgacgg cgccccctgc gcggcgcgtt ccagcaggtc ggtattggtc
     3541 gagccggttt cgtccacgac ttcgatgtcg aagcgcgcct gtccgccgaa ttgcgccagg
     3601 atctggtcac gggacaacca ctgcaccggc gttaccaggc ggtagccgcg tccatgcacc
     3661 ttgtggaccg tgagtccgaa tgcatcgatg ttggcgagcg cattcgacac cgagccgcgc
     3721 gtgatcccca gcgccttgcc gatggcttcg ccggaccgga attcaccgtt ttgcaaaagg
     3781 cgcaggactt cgagcgtatg cggagtcacg tcaaaagagt actgtgagtc gggcgaacta
     3841 tacccgaagc atcgccggta gaatgaccgg aaaacctcgg aggagacgga aagtgtcacg
     3901 acctcaactc gggcacatcc ggcgcgcgtg ccacctttgc accgcagcgc tgctgcttgt
     3961 tttctttgcg gcctgcgaca aggaacgggc atatccggcg cggccggtca acttgatcgt
     4021 tccgtttccc gccggcggct cttccgacct gatcgcgcgc ctggtgtccg agcgagccgg
     4081 gcgcgctttg ggtcagcaga tcgtcatcga gaatcgtccc ggtgccggcg gcaacatcgg
     4141 gaccgaggcg gcggcccggg ccgcgccgga cggctatacg ctgatccagt gcacgatcgg
     4201 cacctgcgcg atcaatcttg cgatctacca tgaccttccg tacgacctgg aacgcgattt
     4261 cgcaccgatc gtattgttcg gctcgatggc gaacgtgctc accgtgaacc cggcggtgaa
     4321 ggccaacagc gtcgcggaac tggtcgcgct ggccagaaag cagtccagca agctgacgtt
     4381 cggctcatca ggctacggct ccagcccgca tctgtctggc gagctgttca agcaggcggc
     4441 cggcatcgac atcctccatg tgccgtatcg cggctcggcg ccggcaatca ccgatctgcg
     4501 tggcggacag atcgacatgt tcttcgacaa cactccctcc atccttccgc acatcaaggc
     4561 gggcgcattg cgcgcgcttg cgatcaccgg cgcgcaacgt tcgccccgcc tcccggacgt
     4621 gccgacgatg caggaggcag gcttccggga ttttgtgatc gctccgtggt tcggcgtgat
     4681 ggcgcagcag aaaaccccgg cgcgcatcat cgagcgcttg aacaaggcgt tcaacgaggc
     4741 gctgtccgat cccgcggtcc tcaggcagtt cgcggacatg gacgtgaaag cgggcggcgg
     4801 aacgcccgag gagttccgcg cgcatatccg cgcggaaacg aggcgctggg gcgacctggt
     4861 gcgcgcgcgc ggcataaggg ccgaagaatt gagataggga atggacctgc aatgaaaatc
     4921 acggcaatcg aaaccctgcg cctgccggaa cgccccaact tgttgctggc gcagatccat
     4981 accgacgagg ggctggtggg tctgggcgaa acctcgcgtg gcgcgcaggc ggtggaagca
     5041 caacttcacg atctggttgc gccgtacctg ctgggcaagg atcctcggga gatccagaaa
     5101 catagcaaac acctgatggg aagctatctg ggttttgcca gcagcagtac cgagatccgt
     5161 gccgcatccg tggtggacat cgcgctctgg gatatcctcg gccaggcgct caatcagcct
     5221 attcatacgc tgctcggcgg cctgacgcgc gaccgcatcc gcgcctacaa cacctgcgcc
     5281 ggctacaact acaacacccg cagcgtggtg cggcgttcgc tgcgcgtggg cgagccggcg
     5341 ccgaagtccg aaggccgcta cgacgaccag atcgccttcg tgcatcgcgc cgacgaactg
     5401 gcgcaaagcc tgcttgaaga aggctacacc gcgatgaaga tctggccctt cgacccctac
     5461 gccgaggaca acggcggcgg cttcattccg gagcgcgatc tcgacattgc gctggaaccc
     5521 tggcgcaaga tccgcagggc cgtcggcgac acaatcgagg tcatggcgga atttcattca
     5581 ctgtggaacc tgcagcaggc gaagcagatt gccagggcgc tggaacctta ccggccgtac
     5641 tggtcggaag acccggtcag gatgtgcgac gtggacacac tcgccgagta cgcgcgctcg
     5701 accacgatcc cggtgtgcgc cagcgaaacg ctgggtggga tgtatccgtt ccgcgacgtg
     5761 ctcgccaacc aggcggccga catcgtcatg ctcgacatcg gctggtgcgg gggcttgacc
     5821 gaagcgcgca agatcgccgc gcttgccgaa gcctggcagc gcccggtggc gccgcatgac
     5881 tgcaacggtc cggtggtctg ggtcgcctcc atccatttca tggcgcacat ccctaacgct
     5941 ctgatcatgg aagtggtgcg cgcgtatttc tccacctggt acaaggacat tctcaccgag
     6001 ctgccgctgg tgaaagacgg ctacattcgt ccgctgaacg gtccggggct gggcacgcgg
     6061 ctgcttccgg atttggtcac gaggcagaac tccatcgtca ggcgcagcgg cgcctgatcg
     6121 ctcgaccgcg ccggtcgccg gggaacggcg ttacaatcgc ggcgggatgc gcgctgccgg
     6181 tacagcggat ggcgcgcaaa cggcaacccg gcaagaacta tccgccgacg caatactcag
     6241 acgttccata caacccgaca atctgatctt catgcgctgg ctcgcaatct gcctcatcct
     6301 cctgattaca gcgctcacct cgatggcgcc ggtatcggcg acgccggtgc agcgtccgca
     6361 catcgaagcg gaacttatcc ccgaaaaatc cagtctgcgc cccggagcgc cactgaccgt
     6421 ggcgctgcgg ctgaagatgg agaaccgctg gcacacctac tggaagaatc ccggcgattc
     6481 gggtctcccc accagaatca gctggacgct gccccagggt ttttcggccg ggccggtcca
     6541 gtggccggcg ccacggcgca tcaatgtggg gccgctcacc aacttcggtt acgagggcgg
     6601 aatattcctg ctgaccgaca ttcaggttcc tgccggactt gccgccggca ccgccgtgcc
     6661 gatcaccgcg aaggcagact ggctggtgtg cgaggaaatc tgcatccccg gcgatgcgac
     6721 cttcagcttc atgctgccgg tgagcaacga gccggcggcg gccgacccga agtgggcaaa
     6781 ggcaatcgag cgcacccgcc agcagttgcc gcgcccgttg gaaggctgga acctcgatgc
     6841 ctacctgcgc ggcgatgatc tggtgctgcg gctgtcgagc gggcgggcac gggtggtgcg
     6901 cattcacaac ctcacgctct ttcccgacca ggacggctgg atcgaaaacg cctccccgca
     6961 gcggatgatc aggaccacgg gcggctatga gttgcgcgtt gcctccgcac ccgcgatccg
     7021 ttcgaagtcc ggcccgttga ccggcctgat cgtcgccgaa cccggtctcg cgcagggtgt
     7081 tgctgccgca gtggtggaag tgccggtcga gtctgcctcg gcactggccg acgagatcct
     7141 cgagcaaccg gccttgagcc cgttcgccga tgtcggactg ttggctgcgc tcggactggc
     7201 gttcctgggc ggtgtgattc tcaatctgat gccctgcgtg ttcccggtgg tttcgatcaa
     7261 ggtgctcagc ttcgtcgaac aagcgcgcgg caatccggca aggctgcgcg cgcacgggtt
     7321 ggcatttgcg gcaggggtgc tggtgtcgtt ctgggtggtg gctggaatgc tgctggcgct
     7381 gcgcgcgcaa ggtgaggcgc tgggctgggg ttatcaactg cagtcgccgt cggtagtggc
     7441 cgcgctggcg ttgctgttct tcgtgctggc gctcaacctg agcggtctgt tcgaggtcgg
     7501 gacgtcgctg cagtcgctgg cggcaaaatt gcggttgcgc aacgattatg cggactcggc
     7561 gttgtccggt ttgctggcga cgctcgttgc cacgccgtgt acggcaccat tcatgggcgc
     7621 ggcgctcggc tttgcgctaa cgcaaccggc cagcgaggcg atgctggtgt tttccgctct
     7681 cgcgctgggc atgtccagtc cttacctgct gttgtgcttc tggcccgccc tgatcaggcg
     7741 cattccgcgc cccgggcgct ggatggagac gctgaagcag gtgctcgcct ttccgctttt
     7801 tctgacggtg gtctggctgg cgtgggtgct gggaaaacag gcgggcgtgg atgccgtggc
     7861 gcgtctgctc gcggggctca cactgatcgc ggccgcactg tggatgattg gtcgctgggg
     7921 tgcttccacc gaaaccggca agcgcgcggc aacggtcgtc gcactcgcgc ttgccgtcgc
     7981 gggctgtgtg ttcgcgtggc ccggcaacaa gacggccgat gccgtcgctg aagaagtcac
     8041 cgggccgtgg aaacggtggt ccccggatgc gcttgcctcg gcacgggccg aaggacgcgc
     8101 ggttttcgtc gacttcaccg cggcgtggtg cgtcacctgt caggtgaaca agcgcctggt
     8161 gctggaatcg gacgccttgc acgcccgctt caaggaacgc aacgtagtgc gactgcgtgc
     8221 ggactggacc aaccaggatg cgcaaatcac cgctgcgctg cgcgacctcg gacgcattgg
     8281 cgtgccggtg tacgcggtct atgcggcggg cagcaagggc gcgccgatct tgctgccgga
     8341 attgctcacc cgggaacggg tgctgcgcgc aatcgatgta gcggcgccgc cctcgtaaaa
     8401 ggattgtttg tcgcggatcg atgacaggag gaaacatgag caagctcgtt gctacgttct
     8461 tggcagtatc gatggccgcg ctggtctctg cggcgcaagc cgcagttgca cccggccagg
     8521 ctgcgccgga attcagcctg gtggatatca acggcagggt gcaaaagctc tccgactatc
     8581 gcggcaagta cgtggtgctg gagtggttca actccgagtg cccgttcgtg cagaagcact
     8641 acgaaagcgg gaacatgcag gcgctgcagg ccaggtacac acagaagggc gtggcgt
//
LOCUS       DASQOP010000363         8208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1000270, whole genome shotgun sequence.
ACCESSION   DASQOP010000363 DASQOP010000000
VERSION     DASQOP010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8208
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000270"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..160
                     /locus_tag="VE030_14090"
     CDS             5..160
                     /locus_tag="VE030_14090"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZF24583.1"
                     /translation="MHSTEEFAGTGVGLAIVQRVVTRHGGSVRADGKVGEGATFTFTL
                     PAGKHDG"
     gene            153..599
                     /locus_tag="VE030_14095"
     CDS             153..599
                     /locus_tag="VE030_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZF24584.1"
                     /translation="MDDFHQIELLLAEDNPRDAEMTMRALRKVNFANKVHWVKDGEEA
                     LDFLYCTGAYSDRQPGHAPKLILLDIKMPKIDGIEVLRRVKGDERTRTIPVVVMTSSN
                     EERDVLESYRLGVNSYIVKPVNFPAFVETVANLGLYWILTNRTPQT"
     gene            complement(781..2364)
                     /locus_tag="VE030_14100"
     CDS             complement(781..2364)
                     /locus_tag="VE030_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HZF24585.1"
                     /translation="MTGLSQADHSVSPPSITIPRDYNAAHDLIERNLAARRANRIAYI
                     DDTGRYTYGELAQRVNRAANALYSLGLGMEDRILLAHLDTIDFPSVFLGAIKAGIVPI
                     TANTLLTTADYKHMLEDSRARALIVSEALLPNFEPLLKSLPLLEHVVVSGKDPHGKAH
                     LQSLLAKAATDFEAAPTSCDDVCFWLYSSGSTGTPKGTVHIHSSLIQTAELYAKPVLG
                     IRESDIVFSAAKLFFAYGLGNSLTFPLAVGATTVLMAERPTPAAVFKRLREQRPTIFY
                     GVPTLYAALLASPDLPRKEEVALRICTSAGEALPADIGRRWTEHFGVELLDGIGSTEM
                     LHIFLSNRPGDVRYGTTGKPVPGYRIRLVDEEGREIGPNEIGELQISGPTSAILYWNN
                     RAKTLATFVGPWTRAGDKYTVDDDGYYTYGGRSDDMLKVSGMYVSPFEVEAALLTHPD
                     VLEAAVVGAMDEQQLVKPKAYVVTRPGATGTAALAEALKQHVKDRLAPYKYPRWIEFI
                     PELPRTATGKIQRFKLRKA"
     gene            complement(2697..3164)
                     /locus_tag="VE030_14105"
     CDS             complement(2697..3164)
                     /locus_tag="VE030_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HZF24586.1"
                     /translation="MKLTILVGTMTGTAQLVAQELELRLDDGELQAQTRLMDGLDAAV
                     FAGGGLFLICTSTYGQGDVPDNAKQLYESLQSARPDLSNVQYGVIALGDRTYAETFCN
                     GGKRFDRILSELGARRIGEIMLHDASAGTMPEEVAAEWVQGWIALCRDQLVDA"
     gene            3279..4184
                     /locus_tag="VE030_14110"
     CDS             3279..4184
                     /locus_tag="VE030_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZF24587.1"
                     /translation="MGARDPKTAAPNLSADHAAFLQKLGERVRETRARRGMTRRILAR
                     DSGVSERYLAQLEAGQGNVSVLLLRQIGDALGIPLNDLLRDDDNQPVELTLIQQFLQR
                     LPKQRLARIRAQLLRDFGSPPADRGKRIALIGLRGAGKSTLGSALARKLDMPFVELDQ
                     EIERDAGISLSEIFLLYGQQGYRRYERRCLERVIESHERCVIATGGSIVSEPATCDLL
                     LSTCFTVWLKAAPEEHMARVVAQGDMRPMAGNAQAMEDLRRILDGRGMLYGQADATVD
                     TAGQTVEQSLRALKKAVSLKEVKPS"
     gene            4181..5866
                     /gene="boxC"
                     /locus_tag="VE030_14115"
     CDS             4181..5866
                     /gene="boxC"
                     /locus_tag="VE030_14115"
                     /EC_number="4.1.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-epoxybenzoyl-CoA dihydrolase"
                     /protein_id="HZF24588.1"
                     /translation="MNSVLEKTPPEPQRPTLYDTHPEKYVHWRLSVEGAVATLSMDVN
                     EDKGLKPGYKLKLNSYDLGVDIELYDALNRIRFEHPEVKCVVITSAKERMFCSGANIY
                     MLGLSSHAWKVNFCKFTNETRNGIEDSSRHDGLKFLAAVNGTVAGGGYELALACDEIL
                     MIDDRSSTVSLPEVPLLGVLPGTGGLTRITDKRRVRRDLADVFCSTSEGVRADRAQQW
                     QLVDRIAKPQQFAEAVKERTKALVAQSKRPGSGIGVALTALARTIDAAGYHYGNVDVA
                     IDRKTRTATITLRAPAAPQPADLDGIHAAGSGWWPLAMARELDDAILMLRTNDLDAGI
                     WILKTRGDADAVLAIDAALERHRDDWFVREVTGMLRRTLARFDVSSRTLFAIIDQGSC
                     FAGTLAEMAFAADRSYMLLLPEGGADTPRMVLSETNFGAYPMVNHLTRLATRYCGDEK
                     TIATLRARIGDRLDAETARELGLITFAPDDLDWDEEVRIAIEERTSLSPDALTGLEAN
                     LRFAGAETMETRIFGRLSAWQNWIFNRPNAVGEQGALKVFGTGRKAKFNWERV"
     gene            5914..6270
                     /locus_tag="VE030_14120"
     CDS             5914..6270
                     /locus_tag="VE030_14120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HZF24589.1"
                     /translation="MKRNHRSLKAWQESIVLVEHVYEATRTFPKEELFGLTSQMRRSA
                     VSVPANIAEGSARTGTKELLQFLSIGAASLSELDTHVEIAIRLGYLKDDALTREIDDV
                     AGLLMGLIASIKRRST"
     gene            6381..7790
                     /gene="boxB"
                     /locus_tag="VE030_14125"
     CDS             6381..7790
                     /gene="boxB"
                     /locus_tag="VE030_14125"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HZF24590.1"
                     /translation="MTINYSERIPNNVDLANDRTLQRALEHWQPRFLDWWREMGPNAF
                     SSADVYLRTATGVDAQGWATYGSVKMPDYRWGIFLAEPAPERTIGFGDNFGQPAWQQV
                     PGELRSQFRRIIVTQGDTEPASVEQQHLLGLTCPSLYDLRNLFQVNVEEGRHLWAMVY
                     LLHAYFGRDGREEAEEMLARHSGDADKPRILGTFNEPINDWLSFFMFTYFTDRDGKFQ
                     LKSLAESGLDPLARTCRFMLTEEAHHMFVGETGVGRVVKRTLEAMKELGSDDPAAVRA
                     QGAVDLPTIQRYMNFWFTSSLDLFGSEVSSNAASSFANGIKGRPDEAQYQDHICSEAS
                     YELETPQGKEAVSLRNAMNEVLRNAYIKDCEIGVKRWNMQISRAGFDFQLRLPSPRFR
                     RAIGAWANAPTDPQGNAISREEFERNMAGWIPSQDDRAFVMSLMQRVVEPGKMAGWIA
                     PPDRGINNLPVDYEYVKLN"
     gene            complement(7804..>8208)
                     /locus_tag="VE030_14130"
     CDS             complement(7804..>8208)
                     /locus_tag="VE030_14130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="epimerase"
                     /protein_id="HZF24591.1"
                     /translation="VPALIENAKKAGVGLHIGKGENIWANVHIDDVTELYLLALQKAP
                     AGALYYAENGEEVLKNVAASISRMLGFGGKTKEWSPQEAEAALGPKAHSSFGSNSRVR
                     GKRSRAELRWKPKGRPILEEIEHGHYNRVHHA"
BASE COUNT         1605 a         2533 c         2532 g         1538 t
ORIGIN      
        1 gcggctgcac agcaccgaag agttcgcggg gacaggcgtc ggcctggcga tcgtgcaacg
       61 cgtcgttacc aggcatggcg gcagcgtgcg cgccgacgga aaggtcggcg agggagcgac
      121 cttcactttc accttaccgg cgggaaaaca cgatggatga ttttcatcag atagaactgc
      181 tccttgcaga ggacaacccg cgagacgccg agatgacgat gcgtgcgctg cgcaaggtga
      241 atttcgcgaa caaggtacac tgggtcaagg acggggaaga agcgctcgat tttctctact
      301 gcaccggcgc atactcggac cggcagccgg ggcacgcgcc gaagctgatt ctgctcgaca
      361 tcaagatgcc gaaaatcgat ggcatcgaag tgcttcgccg ggtcaaaggc gatgaacgca
      421 cacgtacaat accggtggta gtcatgactt cgtcgaatga agagcgagac gtcctcgaga
      481 gttaccggct cggggtcaac agttatatcg tgaagccggt gaatttccct gctttcgtgg
      541 aaaccgtcgc gaatctcgga ctgtactgga tcctgacaaa ccgcacgccg caaacataga
      601 tacgctacac cgaggcgccg gcaggtgcac ccgcaccgaa gagaaattcg cgtctgcgtt
      661 gattctgaat ttcccggtcc ttgttgttct gcttgtcctg aaatttaccc ctcacccctc
      721 gcgccttcac ccttcactca ttcactcatt cactcattca cccattcacc cattcaccct
      781 tcacgccttt cgcaatttga acctctgaat cttgccggtc gcggtcctgg gcagctcggg
      841 aatgaactcg atccagcgcg gatacttgta aggcgccagg cggtccttga cgtgctgctt
      901 gagcgcctcg gcaagcgcgg ccgtgccggt cgcgcccggc ctggtgacga cataggcctt
      961 gggcttgacc agttgctgtt cgtccatcgc gccgaccacc gccgcttcga gaacgtccgg
     1021 atgcgtcagc agcgcggcct ccacctcgaa gggtgaaacg tacatgccgc tcaccttgag
     1081 catgtcgtcg gaacggccgc cgtaggtgta gtaaccgtcg tcatcgaccg tgtacttgtc
     1141 gccggcacgg gtccaggggc cgacgaaggt cgcaagcgtc ttggcccggt tgttccagta
     1201 caggatcgcg ctcgtcggac cgctgatctg cagttcgccg atttcgttgg ggccgatttc
     1261 tcgaccctcc tcgtccacca ggcgaatccg gtagccgggc accggcttgc cggtggtgcc
     1321 gtagcgcacg tcgcccggac gattggaaag aaagatgtgc agcatctcgg tggaaccgat
     1381 gccgtcgagc agttcgacgc cgaaatgctc ggtccagcgc ctgccgatat cggcgggaag
     1441 cgcttctccc gccgacgtac agattcgcag cgcgacttct tccttgcgag gcaagtccgg
     1501 gctcgcgagc agggccgcgt ataaggtcgg gacgccgtaa aagatggtgg ggcgctgctc
     1561 cctgaggcgc ttgaatacgg cggcaggcgt aggccgttcc gccatcagca ccgtggtcgc
     1621 gcccaccgcc agaggaaacg taagcgaatt acccagaccg taggcgaaga agagcttcgc
     1681 tgcggaaaac acgatgtcgc tttcgcggat gcccaggacc ggcttggcat agagctccgc
     1741 agtctggatc aggctggaat ggatgtggac tgtccccttc ggcgtgccgg tcgagcccga
     1801 agaataaagc cagaagcaga cgtcgtcgca gcttgtcggc gccgcctcga aatcggtggc
     1861 agccttcgcc agcagcgatt gcaggtgcgc tttcccgtgc ggatccttgc cggacactac
     1921 gacgtgctcg agcaaaggca ggcttttaag cagcggctcg aaattcggca gcagcgcctc
     1981 cgacacgatc aacgcccgcg cgcgactgtc ttcgagcatg tgcttgtagt cggcggtagt
     2041 aagcagggtg ttggcggtga tcggcacgat gccggccttg atcgcgccga ggaagacgct
     2101 ggggaaatcg atcgtgtcca ggtgcgccag caggatgcgg tcttccatgc ccaggccaag
     2161 cgagtagagc gcgttggcgg cgcggttcac ccgctgcgcc agctctccat aggtatagcg
     2221 gccggtgtcg tcgatgtagg cgatccggtt cgcgcgccgc gcggcaaggt tgcgttcgat
     2281 caggtcatgc gcggcgttgt agtcgcgcgg aatggtaatg ctgggcgggc tgacgctgtg
     2341 atccgcctgg cttaatccag tcacgccttc tccccccgct ttcgctctga tcagggcaga
     2401 tattgcctct cgttccagga cgaaatatac cagccgcccc cccatcatgc tcggctggcg
     2461 ccagctcgaa cttcaaatcc tcaaggcaca atcataaatc tcaccacgaa gacacgaagg
     2521 aacacgaaga aagaacgtgg ctagattttc gttgcgtccc tttgcgttct ttgagccttt
     2581 gcgtcgagaa ggctgttatt tgtcttgcct ctctttgtgt cacttcgtgg tcttcgtgtc
     2641 ttcgtggtga gcgttccggt ttattctcct atgcgccttt gcgtcaaatg ccgctctcag
     2701 gcatcgacca gttgatcgcg gcacaaagcg atccagcctt gcacccattc ggcggcgact
     2761 tcctcgggca tggtgccggc gctggcatcg tgcagcatga tctcgccgat gcgccgcgcg
     2821 ccgagttcgg aaagaatgcg gtcgaaacgc tttccgccgt tgcagaaggt ctcggcgtaa
     2881 gtgcgatcac ccagcgcgat caccccgtat tgcacgttgg acaaatccgg ccgcgcactt
     2941 tgcagcgact cgtaaagctg cttggcatta tccggaacgt cgccctggcc gtaggtcgag
     3001 gtgcagatca ggaacaggcc gccgccggca aagacggccg cgtccagccc gtccatgagc
     3061 cgggtttgtg cctgcaattc gccgtcgtcg agccgcagct ccaattcctg cgcaaccaat
     3121 tgggcggtgc cggtcatggt gccgaccagt atcgtgagct tcattgccac gctcggggtc
     3181 tgcggaggat gaattccatt gaattcgcct gtatatgtat tatcatgcca ctttaccatc
     3241 agataagcag tatattgcat tatcgtccgg gagtcccgat gggcgcacgc gatccgaaga
     3301 cggcggcacc gaacctgagc gcagaccatg cggcattcct gcagaagctc ggcgagcgcg
     3361 tgcgcgagac gcgcgcacgg cgcggcatga cccgcaggat cctggcgcgc gattccggcg
     3421 tctcggagcg ctacctggcc cagctcgaag ccgggcaggg aaatgtctcg gtgctgttgc
     3481 tgcgacagat cggcgacgcg ctcggcattc cgctgaacga tctgttgcgc gacgacgaca
     3541 atcagccggt ggaattgacg ctgatccagc agttcctgca gcggctgccg aagcaacgtc
     3601 tcgcgcgcat ccgtgcgcag ttgctccgcg atttcggcag ccccccggcc gaccgcggta
     3661 aacgcatcgc gctcatcggc ctgcgtggtg cgggcaaatc cacactgggc tcggcgttgg
     3721 caaggaagct cgatatgcct ttcgtggagc tggaccagga gatcgagcgc gatgccggaa
     3781 tcagcctctc ggaaattttc ctgctctacg gccagcaagg ctaccggcgt tacgagcgcc
     3841 gctgcctgga gagggtcatc gaaagccacg agcgctgcgt gatcgccacc ggcggcagca
     3901 tcgtgtccga gcccgctacc tgcgacttgc tgctgtcgac ctgcttcacc gtgtggctca
     3961 aggcggcgcc cgaggaacac atggcacggg tggtggccca gggcgacatg cggccgatgg
     4021 ccggcaacgc gcaggccatg gaagacctgc gccgcatact cgacggccgc ggcatgcttt
     4081 acggccaggc ggatgcgacc gtggacacgg ccggtcaaac ggtcgagcag agtctaaggg
     4141 ccctgaaaaa ggccgtcagc ctgaaagagg tgaaaccatc atgaactccg ttctggaaaa
     4201 aactccgccc gagccgcagc ggcccacgct gtacgacacg caccccgaga agtacgtcca
     4261 ctggcggctg tccgttgagg gcgcggtggc gacgctgtcg atggacgtga acgaggataa
     4321 gggtctcaaa cccggataca aactgaagtt gaactcctac gacctgggcg tggacatcga
     4381 actctacgac gcgctcaacc gcatccgctt cgaacatccc gaggtcaagt gcgtggtgat
     4441 caccagcgcg aaggaacgca tgttctgctc cggcgccaat atctacatgc ttgggctctc
     4501 cagccatgcc tggaaagtga acttctgcaa attcaccaac gaaacgcgca acggcatcga
     4561 ggactcgagc cgccacgacg gactgaagtt cctggccgcg gtgaacggta cggtcgccgg
     4621 cggaggctac gagctggcgc tggcttgtga cgagatcctg atgatcgacg accgttcttc
     4681 caccgtgagc ctgccggagg tgccgctgct gggtgtgctg cccggcaccg gaggactgac
     4741 gcggattacc gacaagcggc gcgtgcggcg cgatctcgcc gacgtattct gctcgacctc
     4801 ggaaggggtg cgcgccgatc gtgcgcaaca gtggcaactg gtggatcgga tcgccaaacc
     4861 gcagcaattc gcggaagccg tgaaggagcg taccaaggcg ctcgtcgcgc agagcaaacg
     4921 tcccggatcc gggataggcg tcgcgctgac tgcattggca cgcaccatcg atgcagccgg
     4981 gtatcactac ggcaacgtcg acgtggcgat cgatcgcaag acacgcaccg cgacgatcac
     5041 gttgcgagca cccgctgcgc cgcaaccggc ggacctcgac ggcatacacg ctgccggctc
     5101 cggctggtgg ccgctggcga tggcgcgcga actggacgac gcgatcctga tgctgcgcac
     5161 caatgatctg gacgccggca tttggattct gaagacgcgc ggcgacgcgg acgcggtact
     5221 cgcgatcgac gccgcgctcg agcggcatcg ggatgactgg ttcgtacgcg aagtaaccgg
     5281 aatgctgcgc cggacccttg cccgtttcga cgtatcctcg cgcacgctgt ttgcgatcat
     5341 cgatcagggc tcctgtttcg ccggcacgct tgccgagatg gcattcgccg ccgaccgcag
     5401 ctacatgctg ctgttgcccg aagggggggc agatacgcct cgcatggtcc tgtcggaaac
     5461 gaacttcggc gcctacccga tggtgaatca cctgacgcgg ctggctaccc gctactgtgg
     5521 cgacgaaaaa accatcgcga cgctgcgtgc acgcatcggc gacaggctcg atgccgaaac
     5581 ggcccgggaa ctcggcctga tcacgttcgc gccggacgac ctcgactggg acgaggaagt
     5641 gcgcatcgcc atcgaggaac gcaccagcct ttcgccggac gcgctcaccg gattggaggc
     5701 gaacctgcgc ttcgccggcg ccgaaaccat ggagacgcgc atcttcggcc ggctctccgc
     5761 atggcagaac tggatcttca accggccgaa cgcggtcggc gaacaagggg cgttgaaagt
     5821 gttcggaacc ggtcgcaagg cgaagttcaa ctgggagagg gtgtgaatga ggagtgaagg
     5881 gtgaaggcgt gaaggggaaa gggtaactgc acagtgaagc gcaaccaccg atcattgaag
     5941 gcttggcagg aaagtatcgt actcgttgaa cacgtctatg aagcgacacg aacatttcct
     6001 aaagaggagc tattcggtct tacgagccag atgcgcagat cggctgtttc ggtcccggcc
     6061 aacatcgccg agggatcagc gcggaccggc accaaggaac tgcttcagtt tctctctatt
     6121 ggagcggcgt ctttgtccga gctcgacacg catgttgaaa tcgccatccg gctcggttat
     6181 ctgaaagacg acgccttgac gcgagaaatc gatgatgttg ccggcttgct gatgggactg
     6241 atcgcatcga tcaagaggcg gtcaacctag ccaggtcttg aaccaccctt cactcattca
     6301 cccttcaccc ttcacgcctt ccccctgccc ccttcaccct tcacccttca cccttcacac
     6361 attcactcgg cggagccgcc atgaccatca actatagcga acgcattccg aacaacgtcg
     6421 atctggcgaa cgaccgcacg ctgcagcgcg cgctcgagca ctggcagccg agatttctcg
     6481 actggtggcg ggagatggga ccgaacgcgt tttcatcggc ggacgtttac ctgcgcaccg
     6541 ccaccggcgt cgatgcccag ggctgggcca cgtatggttc ggtgaaaatg cccgactacc
     6601 gctgggggat ctttctcgcc gagccggcgc cggagcgcac catcggcttc ggcgacaact
     6661 tcggccagcc agcctggcag caggtgccgg gcgagctgcg ttcgcagttc cgccgcatca
     6721 tcgtgaccca gggcgacacc gagccggcat cggtggagca acagcacttg ctgggtctca
     6781 cctgcccgtc cctctacgac ctgaggaacc ttttccaggt gaacgtcgag gaaggccggc
     6841 acttgtgggc tatggtctat ctgctgcatg cctatttcgg ccgcgacgga cgcgaggagg
     6901 ccgaggaaat gctggcacgc cactcgggag acgccgacaa gccgcgtatc ctcggcacgt
     6961 tcaacgaacc catcaacgac tggctgtcgt ttttcatgtt cacgtacttc accgaccgcg
     7021 acggcaagtt tcagctcaag agcctcgccg aatcgggtct cgatccgctg gcgcgcacct
     7081 gccgcttcat gctcaccgaa gaggcgcacc acatgttcgt gggcgagacc ggcgtgggtc
     7141 gtgtagtgaa gcgcacgctg gaggccatga aagagctcgg cagcgacgat cctgccgcgg
     7201 tccgtgcaca gggtgcagtc gatctgccga ccatccagcg ctacatgaat ttctggttca
     7261 cctcttcgct ggatctgttc ggttccgaag tctcttccaa cgccgccagt tccttcgcca
     7321 acggcatcaa gggccggccg gacgaagcgc agtaccagga tcacatctgc agcgaagcga
     7381 gctacgagct ggagacaccg cagggaaagg aagcggtgag cctgcgcaac gcgatgaacg
     7441 aggtgttgcg caacgcctac atcaaggact gcgagatcgg ggtcaagcgc tggaacatgc
     7501 agatcagccg cgcgggattc gactttcagc taaggcttcc gagcccgcgt ttccgccgcg
     7561 ccatcggcgc ctgggcaaac gcaccgaccg atccgcaggg caacgccatc agccgcgagg
     7621 aattcgagcg caacatggcg ggatggattc cgagccaaga cgatcgcgcg ttcgtgatga
     7681 gcctgatgca gcgcgtggtg gagccaggca agatggctgg ctggattgcc ccgccggacc
     7741 gcggcatcaa caatctgcca gtggactacg agtacgtaaa gctgaactga gagcgggttg
     7801 ggactaggcg tgatggacgc gattgtagtg cccgtgctcg atttcctcga gaatcggccg
     7861 gcctttcggc ttccagcgga gttcggcacg agagcgtttc cctcgcaccc ggctgttcga
     7921 tccaaaagat gaatgcgcct tcggcccgag cgcggcttcc gcttcctgcg gggaccattc
     7981 ctttgttttt ccgccaaagc caagcatgcg gctgatggac gcagctacgt tcttaagaac
     8041 ctcctcgcca ttctcggcgt agtagagcgc gccggcaggc gctttctgca gcgcgagcag
     8101 atagagttcg gtgacatcgt cgatgtgtac gttggcccag atgttctcgc ccttgccaat
     8161 atgtaaacca acaccggctt tcttcgcgtt ctcgatcagc gccggcac
//
LOCUS       DASQOP010000364         4238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1402844, whole genome shotgun sequence.
ACCESSION   DASQOP010000364 DASQOP010000000
VERSION     DASQOP010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402844"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="VE030_14135"
     CDS             complement(<1..640)
                     /locus_tag="VE030_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010101791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HZF24592.1"
                     /translation="MRPFLAEVLGTMLLIILGDGVVANVVLNKTKGQNAGWIVITAGW
                     AFGVTVAVYAVGSFSGAHLNPAVTIGLASIGKFAWASVPGYIAAQMLGAFLGATIVWL
                     AYLPHWPVTESQAGKLAVFCTGPAIRNPAANLLTEIIGTFVLVLGVLTILTPKNLNPV
                     HGWDAGFGPFLVGILVWAIGLSLGGPTGYAINPARDLGPRIAHALLPIAGKGN"
     gene            complement(697..3354)
                     /locus_tag="VE030_14140"
     CDS             complement(697..3354)
                     /locus_tag="VE030_14140"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZF24593.1"
                     /translation="MDDAISRLKTALADRYTIERELGRGGMATVYLAHDPKHDRPVAL
                     KVLRPELAAVLGGERFLREISLTAQLQHPHILTLIDSGEADGFLYYVMPYVEGESLRQ
                     RLEREGQLPLDEALRITRAIAAALDFAHGHGVIHRDIKPENIMLHEGEPMVADFGIAL
                     AVSAAGRERLTETGLSLGTPAYMSPEQASAEPRLDGRSDQYSLACVLYEMLAGEPPYT
                     GPTAHAIIAKCLTEPIPHLGTVRRVSPAVETAVTRALAKAPADRFATAGAFVAALTAA
                     STRATRPRWRSAGGALAAVVIIIIGGAAWFYASRNSKLVRNYPNVVPFTSSPGQKFDP
                     VFSPDGNEIAYVWKGETDDTVDLYVKLIGAGGPLRLTSGPAKEYCPSWSPDGRYIAFV
                     RDEAPDSKAAFQLTPALGGAELVRDEASVGKAAYYLIPALGGAERKIGEAYGQAYVFG
                     RCIDWSRDGKYLIVADRMAPQDSRSSILLLSVEDGQRNALVPQPDLYVADPTLSPDGK
                     AVAYAQGAGFIAGDIYVAPASGGRSHRLTSDGRFLAGLAWTPDGREIVFSSDRGGLLR
                     LWRIGVSGGTQELVNGVGEGARSPTISSRGDRLAYIHRRVDANIWRTPGPGWRGRRPA
                     PTRVIASSRADYEAAFSPDGKRIAFASDRSGNVEIWICNSDGTNQTQLTSLKAADSGS
                     PRWSPDGKTIAFDARLEGHGDIFVISVDGGAPRRLTSDPVENNVPAWSRDGKWIYFSS
                     NRTGNWQIWKVLSAGGGAVQVTKNGGFTAQDSPDAEFLYVWLEGGTIWRMPVQGGEPV
                     RVLQGVQLFTWWKVGRHGIYFIDGSTTPAHVRFLDFLTQRVRTITSLDVGYVTPGGSY
                     FDLSPDEQWFVFDRVDQAESDIMLVENFR"
     gene            complement(3424..>4238)
                     /locus_tag="VE030_14145"
     CDS             complement(3424..>4238)
                     /locus_tag="VE030_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24594.1"
                     /translation="TGDIVVDGIWQYQNFIHGRHLQDKKLHFNGNATLRGGWNVGAGY
                     FVESFGYDSALFAAYYLRVPDGSGGVRFVPFTGQPTIGNGEYIVQIGTPQWKHFSGSG
                     YFLQGHDENFFEWASGDLLLLSADLSYRPTERLRNSVSYFWQQVNRRTDGSLVNVGRV
                     LRAKIEYQVSRPLFIRVVGQYIQEKTDSLRDDSRTGAPIYIQGPDSLTRAAASRSNLF
                     HADLLLSYQPVPGTVVFAGYGSNMTDEEAFQFRGLRRTDDAFFLKLSYLFRL"
BASE COUNT          822 a         1342 c         1323 g          751 t
ORIGIN      
        1 aattcccctt gcccgcgatg ggcaggagcg cgtgggcgat tcgcggaccc aggtcacgcg
       61 ccggattgat cgcgtagccg gttggcccgc ccaacgaaag accgatggcc cacaccagga
      121 tgccaaccag gaatggaccg aatcctgcgt cccacccatg taccgggttg agattcttcg
      181 gtgtcaggat cgtcagcacg ccgagcacca gcacgaaggt gccgatgatc tcggtcagga
      241 ggttcgcggc cgggttgcgg atggcggggc ccgtacaaaa caccgccagc ttccctgcct
      301 ggctctccgt gacgggccag tgcggcaggt aggcgagcca cacgatcgtg gcaccgagga
      361 acgcgccgag catttgggct gcgatgtagc cgggcactga agcccatgcg aacttgccga
      421 tggacgcgag accgatggtc accgccgggt tcagatgcgc gccgctgaag ctgccgacgg
      481 catacaccgc gaccgtcaca ccgaacgccc accccgccgt gatgacgatc cacccggcgt
      541 tctggccttt ggtcttgttg agcacgacgt tggcgaccac accatcgccc aggatgatca
      601 acagcatggt gccgagcact tctgcaagga agggtcgcat aggcttgagt ggagaagcgg
      661 cgaagctatc agcgcggcag cgagttgtcc acacgtttag cggaaattct cgaccagcat
      721 gatatcgctc tcagcctggt ctacacggtc gaagacaaac cactgctcat ccggagagag
      781 atcgaagtat gagccgccag gggtcacgta tcccacatcc agcgaagtga ttgttctcac
      841 gcgttgggtc aagaagtcga gaaacctgac atgggcgggg gtggttgacc cgtcgatgaa
      901 gtaaatgccg tggcgaccga ctttccacca ggtaaacaac tgcacacctt gcagaacgcg
      961 aacgggctcc ccgccctgga ccggcattct ccaaatggtc cccccctcaa gccacacgta
     1021 gagaaactcg gcatcgggag aatcctgtgc ggtgaagccg ccgtttttcg tcacctgtac
     1081 cgcaccgcca cccgctgaca gtactttcca aatttgccaa ttccctgtcc ggttcgagga
     1141 gaaatagatc catttgccat cccgagacca cgcggggacg ttattttcga ccggatcgct
     1201 ggtgagacgg cggggggcgc ctccatcgac actgataacg aagatatcac cgtggccttc
     1261 gagccgggca tcgaaagcaa tcgtcttgcc gtccggagac cagcgtggcg agcctgaatc
     1321 ggcagccttc aacgacgtga gttgtgtttg gttggtgccg tcgctgttgc agatccagat
     1381 ttcgacattc ccagagcgat cggacgcgaa ggcgatccgc ttgccatcgg gagaaaaggc
     1441 cgcttcataa tccgcgcgtg atgaagcaat cacacgcgta ggtgcgggac ggcgtcctct
     1501 ccaaccaggt cccggcgttc gccagatgtt cgcatctacg cggcgatgaa tataggctag
     1561 ccgatctccc ctgctagaaa tggtcggcga tctcgcgccc tcacccacac cgttcaccag
     1621 ttcctgggtg cctcccgaga caccgatgcg ccatagtctc aaaagcccgc cacgatctga
     1681 ggagaaaacg atctcccgtc cgtccggcgt ccacgcgagt ccagccagga atcgcccgtc
     1741 ggacgtcagg cgatgtgacc ggccaccgct ggcaggcgca acataaatgt caccagcgat
     1801 aaaacccgct ccttgggcgt aggcgaccgc ttttccatcg ggggagaggg ttggatcggc
     1861 gacgtacaga tcgggctggg ggaccagtgc gttcctttgt ccgtcctcaa ccgagagaag
     1921 caggatgctg gagcgggagt cctgaggcgc cattctgtct gccacgatca ggtacttacc
     1981 gtcgcgagac cagtcgatgc accgcccgaa gacgtaggct tggccatatg cctccccgat
     2041 cttgcgttcg gccccaccca gcgccgggat gaggtaataa gcagccttgc caaccgaggc
     2101 ttcatcgcga acgagttcgg ccccaccgag cgcgggggta agctgaaaag cagccttgga
     2161 atccggggct tcatcgcgaa cgaaggcgat gtaacgcccg tcgggcgacc aagacgggca
     2221 atactccttg gcgggaccgc tggtaagtcg gagcggacca ccagcgccga tgagcttgac
     2281 gtaaaggtcg acggtgtcgt cagtttcgcc tttccagacg taagcaatct cgttgccgtc
     2341 cggcgagaac acaggatcaa atttctgacc gggggaactc gtgaagggca cgacgttcgg
     2401 gtagttccga acgagtttgg aattccggga agcataaaac cacgccgcgc caccaatgat
     2461 gatgatcaca acggcggcga gtgcaccccc cgcggaccgc caccggggac gcgtcgcccg
     2521 agtcgaggcg gccgtgagcg ctgcgacgaa ggctcctgcc gtggcgaatc ggtcggccgg
     2581 cgccttcgcg agtgctctcg tgacggcggt ttccaccgcc ggtgaaacgc ggcgcaccgt
     2641 gcccaggtgc ggtatcggtt cggtcaagca cttggcaatg atggcgtgcg cggtcggacc
     2701 cgtgtagggc ggctcccccg ccagcatctc gtacagcacg cacgccaggc tgtactggtc
     2761 gctgcggcca tcgagccggg gctcggcgct cgcctgttcg ggactcatgt aggccggggt
     2821 cccaagggaa aggccagtct cggtgagacg ttcccgtccc gccgcgctca ccgccagcgc
     2881 gatcccgaag tccgccacca tcggctcgcc ctcgtggagc atgatgtttt caggcttgat
     2941 gtcccgatgg atcaccccgt gtccatgggc gaagtcgagc gccgcggcga tcgcccgggt
     3001 aatgcgcagc gcctcatcga gcggcagctg gccttcccgc tccaagcgct ggcgcagact
     3061 ctcgccctcg acgtagggca tcacgtaata caggaagccg tccgcttccc cggaatcgat
     3121 gagtgtcagg atgtgcgggt gctggagctg cgccgtgagg ctgatctctc tgagaaaccg
     3181 ctcaccgccg agcacggcgg cgagctcggg gcgcagcacc ttgagcgcga ccgggcggtc
     3241 gtgcttcggg tcgtgggcga gatacaccgt cgccatcccg ccgcggccga gctcgcgctc
     3301 gatcgtgtag cggtcggcga gcgctgtctt gaggcgagaa atagcatcgt ccaccggcga
     3361 ccgctcctgg cgtgagcgcg gtccctataa tggggacccg ccggacaggt agctagttga
     3421 gggtcacagc ctgaacaggt agctcagctt gaggaagaag gcgtcgtccg ttcgcctcag
     3481 gccccggaac tggaaggcct cttcgtctgt catgttgctg ccatacccgg cgaacaccac
     3541 cgtccccggc acgggttgat agctgagcag caggtcggcg tggaagagat tgctgcgcga
     3601 cgcagccgcg cgcgtcaggc tgtccggccc ctggatgtat atcggcgcgc cggtccgcga
     3661 gtcgtcgcgt agcgagtccg tcttctcctg gatgtactgg cccaccaccc gaatgaacag
     3721 cggccgcgag acctggtatt cgattttggc ccgaagcacg cgaccgacat tcaccaggct
     3781 gccgtcggtg cggcgattca cctgctgcca gaaatagctg acgctgttcc gcagccgctc
     3841 cgtggggcga taggacaggt ccgcgctcag gaggagtaaa tcgcccgagg cccactcgaa
     3901 gaagttctca tcgtggcctt gcaggaaata cccgctgcca ctgaagtgct tccattgcgg
     3961 tgtcccgatt tggacgatgt actcgccgtt cccgattgtc ggctgtcccg taaacgggac
     4021 aaacctcacg ccgccgctgc cgtcaggcac tctgagatag taggcggcaa acagcgcgga
     4081 atcgtagccg aacgactcca cgaaataacc cgcgcccacg ttccagccgc cgcgtagcgt
     4141 cgcgttgccg ttgaagtgca gcttcttgtc ctgcaggtgc cgcccgtgga tgaagttctg
     4201 atactgccag atgccgtcca ccacgatgtc gcccgtga
//
LOCUS       DASQOP010000365         3217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_2812081, whole genome shotgun sequence.
ACCESSION   DASQOP010000365 DASQOP010000000
VERSION     DASQOP010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2812081"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VE030_14150"
     CDS             complement(<1..384)
                     /locus_tag="VE030_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZF24595.1"
                     /translation="MRFAYLAIPLLALSACAAIHRQEAADSEVLLRQAGFEARAADSP
                     ERQQDLVKMPSRQIVERDQNGAAVYSFADPDNCRCLYVGGQKEYAKLQELRQQRIDDH
                     NQLARRSSFEGGISDLWGPWKPEGLI"
     gene            complement(381..1388)
                     /locus_tag="VE030_14155"
     CDS             complement(381..1388)
                     /locus_tag="VE030_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZF24596.1"
                     /translation="MQFAGIPGTSLEVSRIALGTWAIGGWMWGGSEETESIDTIRAAL
                     DQGITVVDTAPVHGFGRSEEIVGKALAASGLRARAVIATKAGLEWRDGRVFRNASRAR
                     IMREVEDSLARLRTDYIDIYQVQWPDSLVPMEETAAAMASLYNQGKIRAIGVSNFSVA
                     QMDAFRRVAPLHVLQSPYNLFERGIEAEILPYCRRNNIAVLGYGALCRGLLSGRMRPD
                     TTFGADDLRRNDPKFQAPRFDQYLRAVQRLDLLAQYRYGKRVIHLAVRWMLDQGVTAA
                     LWGARHPGQLAPVADVGGWSLEADVKAEIDRVLRERITNPVGPEFMAPPSRFHLTEAQ
                     I"
     gene            complement(1641..2069)
                     /locus_tag="VE030_14160"
     CDS             complement(1641..2069)
                     /locus_tag="VE030_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HZF24597.1"
                     /translation="MDPADVAPRGYGSVVVAFHWLTAVLVLAAFVVGPGGSEQRVYSA
                     ANDFDRQIHEVLGLTVFSLTLLRLVSRAFAPAAQWPSAPRWMNRISKVVQAQIHTILG
                     DTVVWLAGFHAAAALFHHFVLRDEVLLSMLPARWRRRRLS"
     gene            complement(2079..2759)
                     /locus_tag="VE030_14165"
     CDS             complement(2079..2759)
                     /locus_tag="VE030_14165"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HZF24598.1"
                     /translation="MSRTGSELFPLDPQIAQRVVLLSGDSQGEPGSRRRTTRDERFRL
                     RQSIFDMGRACAAELLAGLGSRETSVAMAADRSPVWPQGYVGSITHTENLLGVAVARG
                     KDFRGIGIDAEAIIEPETMVEIDDLYMNERERSLRGSVEINHQTFSSLCFSAKESFFK
                     CLYPLTGVWFDFQDAEIASFDPNLQILQMRLLRDLDSGFRQGQVFGGTFRWTDRRVYT
                     AFTLPSQC"
     gene            complement(2808..2901)
                     /locus_tag="VE030_14170"
     tRNA            complement(2808..2901)
                     /locus_tag="VE030_14170"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2865..2867),aa:Ser,seq:gct)
     gene            complement(2915..>3217)
                     /locus_tag="VE030_14175"
     CDS             complement(2915..>3217)
                     /locus_tag="VE030_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HZF24599.1"
                     /translation="RLLARIDDPEKNWKFSRSDIEERRRWKDYMKAYQGCLRATSKRY
                     ARWYVVPADDKPNARLIVSQIIVDTLRGLHLEHPRLDAGRRRELQAIRRLLIAKRV"
BASE COUNT          607 a         1024 c         1033 g          553 t
ORIGIN      
        1 tatcagccct tccggtttcc acgggcccca gaggtctgaa ataccgccct cgaagctcga
       61 gcgtctggcg agctggttgt ggtcgtcgat gcgctgctgc ctgagctcct gcagcttcgc
      121 gtactccttc tgcccgccga catagaggca gcggcagttg tccggatcgg cgaagctgta
      181 cacggcggcg ccgttctggt cgcgctcgac gatctggcgc gaaggcatct tcacgaggtc
      241 ctgctggcgc tccggactgt ccgcggcgcg cgcctcgaag ccggcctgcc tgagcaggac
      301 ttcggaatcg gcggcttcct ggcggtggat ggcggcacac gcggacaaag cgagaagcgg
      361 gatggcaagg taggcaaatc tcatatctga gcctccgtca ggtggaatcg cgacggcggt
      421 gccatgaact cgggccctac cgggttggtg atccgctcgc gcagcacgcg gtcgatctcg
      481 gccttgacgt cagcctcgag cgaccagccg cccacgtcgg cgaccggtgc aagctggccg
      541 ggatggcgcg cgccccacaa cgcggccgtg acgccctggt ccagcatcca gcgcaccgcg
      601 agatggatga cgcgcttgcc gtagcggtat tgcgcgagca gatcaagccg ctgcacggcg
      661 cgcagatact gatcgaagcg tggcgcctgg aatttcgggt cgttcctgcg caggtcgtcg
      721 gcgccgaacg tcgtgtccgg ccgcatccgg cccgagagca gcccgcgaca gagcgccccg
      781 tagcccagaa cggcgatgtt gttcctgcgg caatacggca ggatttcggc ctcgatgccg
      841 cgctcgaaca ggttgtaggg cgactgcagc acgtgcagcg gcgcgacgcg ccggaaagcg
      901 tccatctgcg cgaccgagaa attgctcacc ccgatggcgc ggatctttcc ctggttatag
      961 agcgatgcca tcgcggcagc ggtttcctcc atcggcacca aggagtccgg ccactgcacc
     1021 tggtagatat cgatgtaatc ggtgcgcagg cgcgcgagcg agtcctcgac ctcgcgcatg
     1081 atccgtgcgc ggctcgcgtt gcggaacacc cttccgtcgc gccactccag gccggctttc
     1141 gtggcgatga ccgcgcgcgc gcgcaatccg gacgccgcca gcgccttgcc gacgatctcc
     1201 tcggagcggc cgaagccgtg gaccggcgcc gtatcgacga cggtgatgcc ttgatcgagg
     1261 gcggcgcgga tcgtgtcgat cgattccgtc tcttcgctgc cgccccacat ccagccgcct
     1321 atggcccagg tgccgagggc gatgcgcgag acttcgagag aggtgccggg gatcccggcg
     1381 aactgcatgc tgaggtcagg tagtatcggc ttgctgccca cgattcagtg ctcctttcga
     1441 gcgccatggt cggcaatgcc ggctacccgt tgattgcaac gcccgcgcga accgttgcag
     1501 gtgcaacgcg caaaggcagg tacgcgtcaa gcgaagggac gaacggcggc tgatgcggtt
     1561 acagtcgaaa cgcgccgttc gtcggatttc gcggcgcgcc gcgggcgaaa gagacccgct
     1621 cgcgatgggc tacggcgagg tcatgaaagg cggcggcgtc tccaccgtgc cggaagcatg
     1681 gacaggagga cctcgtcgcg cagaacgaaa tgatggaaga gcgccgcggc agcgtggaat
     1741 ccagcgagcc agacgacggt atcaccgagg atcgtatgga tctgcgcctg cacgaccttc
     1801 gatatgcggt tcatccaccg cggcgccgaa ggccactgcg ccgccggagc gaacgctcgc
     1861 gaaacgaggc gcaatagcgt gaggctgaat accgtcagcc ccaacacctc gtggatttgg
     1921 cgatcgaaat cgttcgctgc cgaataaacc cgttgctctg acccaccagg acccacaaca
     1981 aacgccgcca gcaccaagac cgcggtgagc caatgaaatg ccacgacgac ggaaccgtag
     2041 ccgcgaggag ctacgtcggc tggatccaaa tcactccatt agcattggga gggcagggta
     2101 aatgccgtgt acacccgtcg atcggtccag cgaaatgtgc cgccgaagac ctggccctgc
     2161 cggaagccag aatcaagatc ccgcagcaat cgcatctgaa gaatctgcag attcggatcg
     2221 aaagaggcga tctctgcatc ctgaaagtcg aaccagaccc cggtcagggg atagaggcac
     2281 ttgaaaaacg attctttcgc cgagaagcac aaggacgaga acgtttggtg gttgatttca
     2341 acagagccgc gcagcgaacg ctctctctcg ttcatgtaaa ggtcatcgat ttcgaccatg
     2401 gtctccggct caatgatcgc ttccgcgtcg attccgatgc ctcgaaagtc tttgcctcgc
     2461 gcgacggcaa ctcccaggag gttctcagtg tgcgtgatcg agccgacata gccctggggc
     2521 cacaccggcg agcggtccgc ggccatggct accgaagtct cgcgagaacc aaggccggcg
     2581 agaagctcgg cggcacatgc cctgcccatg tcgaatatcg actgacgcag gcggaaccgt
     2641 tcgtcccggg tcgttctccg gcgcgagcca ggttcgcctt gggaatcgcc cgagagcaat
     2701 acaacgcgct gcgctatctg cggatccagc ggaaacagct cagagcccgt tcggctcatc
     2761 ttggacaatg tcgcttcggc actcagggcg gaaacggctg aattgtttgg cggagagaga
     2821 gggattcgaa ccctcgatac gggtttaagc ccgtataacg ccttagcagg gcgccccctt
     2881 cggccactcg ggcatctctc cgatgcaggg aattttacac gcgcttggcg atgagcaggc
     2941 ggcggatcgc ctgcagctcc ctgcgccgcc cggcatcgag ccgcggatgc tcgagatgca
     3001 ggccgcgcag cgtgtcgacg atgatctgcg aaacgatcag ccgcgcattc ggcttgtcgt
     3061 ccgcaggcac tacgtaccac cgcgcgtagc gcttgctggt ggcgcgcagg cagccctggt
     3121 aagccttcat gtagtccttc cagcggcggc gctcctcgat gtcggacctg ctgaatttcc
     3181 agttcttctc cgggtcgtcg atgcgcgcca gcaagcg
//
LOCUS       DASQOP010000366         3500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_399105, whole genome shotgun sequence.
ACCESSION   DASQOP010000366 DASQOP010000000
VERSION     DASQOP010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399105"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VE030_14180"
     CDS             <1..283
                     /locus_tag="VE030_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZF24600.1"
                     /translation="VAGIVWGSKLGVQFSLSLVALKALPVLVLGGFDSVLGAIVGGLV
                     IGAGEKLSEVYLGPLVGGGIENWFAYVLALVFLLVRPEGLFGEKHIDRV"
     gene            437..1501
                     /locus_tag="VE030_14185"
     CDS             437..1501
                     /locus_tag="VE030_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZF24601.1"
                     /translation="MFYREAGQFKTSYAADQAVFPILQDRIFIAVWLAIGFVLVPLVS
                     TEYLFRAIFIPFLILSLAALGLNLLVGYCGQLSLGTGGFMAVGAYAAYNLCVRIPGLN
                     PLVAFVLAGGVTTVVGIVFGIPSLRIRGYYLAVATLAAQFFLDWVFSRVAWFTNNAPS
                     GSVTAPPITLFGLTFETPVRKYLLCLAILVLFAVAAKNIVRGRMGRVWMATRDMDVAA
                     EIIGIRPMYAKLSAFAVSSFFIGVAGALWAFVHLGSWEPLAFDINRSFSILFMVIIGG
                     LGSILGSLLGAAFITLLPLLLNQLPQWVGIAISTAMISHLESMIFGALIVFFLVVEPL
                     GLARLWHIGKEKLRLWPFPY"
     gene            1557..2885
                     /locus_tag="VE030_14190"
     CDS             1557..2885
                     /locus_tag="VE030_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZF24602.1"
                     /translation="MEKIFSPKTLMLGVAAAGALLAAAQTFAQASEQFVPALVYRSGP
                     YAPNGVPFANGYADYLNLVDERDGGINGVKIVFEECETGYSTDRGVECYERLKGKGPT
                     GAAAFSPLSTGITYALTEKAPVDKIPLITMGYGRSESVDGSVFAWNFPLLGTYWTAAD
                     VIIQYIGQLNGGLDKLKGKKIALVYHDSPYGKEPIPLLNERAKRHGFELLTFPVTHPG
                     VEQKAAWLQVRQNRPDYVLLWGWGVMNSTAIKEAVAVNYPRDHMIGVWWAGAEPDVLP
                     AGEDAKGYNSIALQHSAGQIAVHADILKNVYDRGKGAGKREEVGYVLYNRGMLNAMLV
                     VESIRTAQGKYGKKPMTGEQVRWGIENLNLDQNRIKQLGFAGTLQPLKVSCHDHEGVR
                     LARIHQWDGVKWKFVSDWIEGDNTLLRPMVDEAAKKYAREKNITPGCLKG"
     gene            3011..>3500
                     /locus_tag="VE030_14195"
     CDS             3011..>3500
                     /locus_tag="VE030_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZF24603.1"
                     /translation="MSTPKQPETALLAVNNIEVIYDHVILVLKGVSLEVHGGRIVALL
                     GANGAGKSTTLKSISNLLRAERGEVTKGSVEFDGERIDQRNPSDVVKRGIVQVMEGRH
                     CFAHLTVEENLLTGAFTRNVGRTEIGVDLDKVYTYFPRLKQRRGSLAGYTSGGEQQMT
                     AVG"
BASE COUNT          671 a         1033 c         1081 g          715 t
ORIGIN      
        1 ggtggccggc atcgtgtggg gatccaagct cggcgtgcag ttttcgctgt ccttggtggc
       61 actgaaggcg ctgccggtcc tggttcttgg cggattcgat tccgttcttg gcgccatcgt
      121 gggcgggctc gtcatcggcg ccggggaaaa gctttccgag gtctacctcg gaccgctggt
      181 cggcggcggc atcgagaact ggtttgccta cgtgctggcg ctggtgttcc ttctcgtcag
      241 gcccgaaggt ctgttcgggg agaaacatat agacagagtc tgaaaaccgg gtgaggcgag
      301 aagcgtgagg cgtaacgaca aaagaataac cggtgtaatc ctgtaggcta tggacattca
      361 ccacagccgg cgcgggctca ttccgactgt tgcaccttca cacttacgcc tcacgcctca
      421 cccctaacgc ctcaccatgt tctacaggga agcgggacaa ttcaagacgt cctatgccgc
      481 cgaccaggcg gtgttcccga tccttcagga tcggatcttc atcgcagtct ggctcgcgat
      541 cggtttcgtt ctggtgccgc ttgtttcaac cgagtatctt tttcgcgcca tattcatccc
      601 cttcctgatc ttgtcgctcg cagcgctcgg gctcaatttg ctggtcggct actgcgggca
      661 gctttcactc ggaaccggcg gtttcatggc tgtgggcgcc tacgccgcct acaacctgtg
      721 cgtgcgcatc ccgggcctca atccgttggt cgcgtttgtg ctcgccggcg gggtaacgac
      781 ggtggtcggc atcgtgttcg gcattcccag tctgcgcatc agaggctatt acctggccgt
      841 ggccaccctg gctgcgcaat tcttcctgga ctgggtgttc agccgggtcg cgtggttcac
      901 caacaacgca ccatcgggtt ccgtcaccgc accgccgata acgctgttcg gccttacctt
      961 cgaaaccccg gtcagaaagt acctgctgtg ccttgcgatt ctggtgttgt tcgcggtggc
     1021 ggcgaagaat atcgtgcgcg gccgcatggg gcgcgtgtgg atggcgacgc gcgacatgga
     1081 cgtagccgcg gaaatcatcg gcatccgccc catgtacgcg aaactttccg cattcgcggt
     1141 gagttcgttc ttcatcggcg tggcgggtgc tctctgggcg ttcgttcacc tgggctcatg
     1201 ggaaccgctc gctttcgaca tcaatcggtc gttcagcatt cttttcatgg tcatcatcgg
     1261 cggcctgggt tcgatcctgg gcagtttgct cggggccgcc ttcattaccc tgctgccgct
     1321 attgctcaac cagctacccc aatgggtcgg catcgccatt tcgacggcga tgatctcgca
     1381 cctggaatcc atgatcttcg gggctctgat cgtttttttc ctggtcgtgg aacccctggg
     1441 actagcgcgg ctgtggcaca tcggcaagga aaaattgcgc ttgtggccgt tcccgtatta
     1501 acttgaagaa gcaaaccgca gccggccgcg gcaccattcc ataaggaggc aacaccatgg
     1561 agaagatttt cagtcccaag accctaatgc tgggagtcgc cgcggcgggc gcgctcttgg
     1621 cggcagcgca gacgttcgcg caggccagtg aacagttcgt cccggcactt gtttatcgaa
     1681 gcggccctta cgcgccaaac ggcgtcccct ttgcaaacgg ctatgcggat tacctgaacc
     1741 tggtcgacga gcgcgacggc ggcattaacg gtgtgaagat cgttttcgag gaatgcgaaa
     1801 ccggctactc caccgaccgc ggcgtggagt gctacgagcg gctgaagggt aaggggccca
     1861 ccggtgcggc tgcgttcagc ccgctctcca cggggatcac ctacgcactg acggaaaagg
     1921 cgccagtgga caagatccct ctgattacca tgggctacgg ccgctccgaa tccgtggatg
     1981 gcagcgtatt cgcgtggaac ttcccgttgc tgggtaccta ttggactgcc gctgacgtga
     2041 tcatccagta cattggccag ctgaacggcg gcctcgacaa gctcaagggc aagaaaatcg
     2101 cactggtgta ccacgactcg ccctacggca aggagccgat cccgctgctt aacgagcgcg
     2161 ccaagcgcca cggcttcgaa ctgctcacgt tcccggtcac gcatccgggc gtggagcaaa
     2221 aggcggcgtg gttgcaggtc cgccagaacc gtcccgatta cgtgctgctg tggggttggg
     2281 gggtgatgaa ctccaccgct atcaaggaag cggtggcggt gaactatccg cgcgaccata
     2341 tgatcggcgt gtggtgggcc ggcgcggaac ccgacgttct gccggcgggt gaagacgcga
     2401 agggctacaa ctccatcgcc ctgcagcatt cggcaggcca gatagcggtg cacgcggaca
     2461 tcctcaagaa tgtgtacgac agaggcaagg gcgcaggcaa acgcgaggag gtggggtacg
     2521 tgctctacaa ccgtggcatg ctgaacgcca tgctggtggt cgagtcgatc cgcactgccc
     2581 agggaaaata tggcaagaaa ccgatgaccg gcgaacaggt gcggtggggc atcgagaacc
     2641 tgaacctcga tcagaaccgt atcaagcaac tcggttttgc cggcacgctg caaccgctca
     2701 aggtgtcgtg ccatgatcac gagggggtgc gccttgcgcg tattcaccag tgggacggcg
     2761 tcaaatggaa gttcgtgtcg gactggatcg aaggcgacaa cacgctgctg cgcccgatgg
     2821 tggacgaggc ggccaagaaa tacgccaggg aaaagaacat cacccccggt tgcttgaagg
     2881 gatgaaacgc cgtgaaggcg tgaaaagtga atgtgtgaag ggtggattac tgcagttcgg
     2941 ggtttcccct tcactcattc acaccttcac ccttcaccga ttgacgcttc acagataacg
     3001 tcccgaagcc atgagcacgc cgaagcagcc ggaaactgca ctgctcgccg tcaacaacat
     3061 cgaggtcatc tacgaccacg tgatcctggt gctgaaggga gtgtcgctcg aggtgcatgg
     3121 cgggcgcatc gtcgcgttgc tgggtgccaa tggcgcgggc aaatccacca cgctcaagtc
     3181 aatctccaac ctgttgcgcg ccgagcgcgg cgaagtcacc aaaggttcgg tggaattcga
     3241 tggcgagcga atcgatcaac gcaatccctc cgacgtggtc aaacgcggca tcgtgcaggt
     3301 gatggaaggg cgccattgct tcgcccacct cacggtggag gagaacctgc tcaccggcgc
     3361 gtttacccgc aacgtaggtc gcactgagat tggcgtcgac cttgacaagg tctacaccta
     3421 tttcccgcgt ctgaagcagc gccgcggcag ccttgccggc tacacctcgg gtggcgaaca
     3481 gcagatgacc gcggtaggcc
//
LOCUS       DASQOP010000367         2608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_1005707, whole genome shotgun sequence.
ACCESSION   DASQOP010000367 DASQOP010000000
VERSION     DASQOP010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005707"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VE030_14200"
     CDS             complement(<1..288)
                     /locus_tag="VE030_14200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase"
                     /protein_id="HZF24604.1"
                     /translation="MEAFKRNGYHFPMRVITEAQAAGYRQRIEAFEAVHGLVMGTPYR
                     NKPHLVFGWANELIHNPRILDAVEDLLGPDLLVWGSSFFIKEPQDPAYVSWH"
     gene            complement(425..1513)
                     /locus_tag="VE030_14205"
     CDS             complement(425..1513)
                     /locus_tag="VE030_14205"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HZF24605.1"
                     /translation="METLRLFKRSAAVLGTLLAFSAAVHAADPSTFPAPVGDRSIIPA
                     GAKLELIFDGGCMLTEGVAAGHDGMIYFSDITFTKFCRDASGKYAQAGNIWKYNPKNG
                     ETTIFRSPSGMSNGIKFDRDGNMIAALGADYGGRMLVKTDMKTGKTYILSGLYNGRPY
                     NALNDITIDEKGRIYFSDPRYLGHEGVYQDGFAVYRLDPDGSVTRVATDCGKCNGVLI
                     SPDQKTLYIIGNDNGWLEFQNLKEGEKTLQGHHQLQAYDVDAASGNLSNRRVLIDYTT
                     RDKPCSGPDGMVADTEGNIWLASRCEYRPGVQVLSPDGKELAYISTGKELPTNVGFGR
                     GADSNLLYLTSGKSLYRIRVGKKGYQLP"
     gene            1672..2484
                     /locus_tag="VE030_14210"
     CDS             1672..2484
                     /locus_tag="VE030_14210"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZF24606.1"
                     /translation="MSIRKSAVREGARYSAPALEKGLDVLELLAAEPHGLNLQEISRR
                     LKRTSNELFRMLDVLVRRGYLVRQPDSAYVLTLRLFELAHRHPGVDRLLDCAMPHMQA
                     LAGRTGQSNHLCVHHDARLVVLAQAEPPEPMSCSVRQGAHFAFHDDRVSARVITAFQT
                     GEQRDQYLSEVLGSRRAAPARRRALIARLEATRRRGYDEGPSDTINGVVDICFPIFDR
                     FGAIAALNIVYLRQRDSRVTIPAAREKLRHTAQAISRSLGWLGRANGRPGDG"
BASE COUNT          488 a          802 c          792 g          526 t
ORIGIN      
        1 gtgccaggaa acataggccg ggtcttgtgg ctccttgatg aagaagctgc tgccccagac
       61 cagcaggtcg ggccccagca agtcctcgac cgcatcgaga atgcgcgggt tgtggatcag
      121 ttcgttggcc catccgaaaa cgaggtgcgg cttgttgcgg tagggggtcc ccattaccag
      181 cccgtgcacg gcttcgaacg cctcgatcct ttgacgatat ccagccgcct gggcttccgt
      241 aatgacgcgc atcggaaagt ggtagccgtt acgcttgaat gcctccacct gcgcctccgt
      301 aagcgctctg cattttttcg ccgtagagac gggttgcgcg ctgctcataa gaacctctcc
      361 tgtccaggaa aaagccgcag ggaccgcgaa ggcccctgcg gcaccattac tgcccccccg
      421 aaaatcaggg gagctgatag cccttcttgc cgacccggat cctgtacagg ctcttgcccg
      481 aggtcaggta caacaggttg gaatcggcgc cacgcccgaa gccgacgttg gtcggcaact
      541 ccttgccggt ggagatgtag gcgagttcct tgccgtcggg actcagcacc tgcactcccg
      601 gccggtattc gcaacgcgag gcgagccaga tgttgccttc ggtgtcggca accatgccgt
      661 ccgggccgct gcaaggcttg tcgcgcgttg tgtagtcgat cagcacccgg cggttgctga
      721 ggttgccgct ggctgcatcc acgtcgtagg cctgcaactg gtgatggccc tgcagggtct
      781 tctcgccctc cttcaggttc tgaaactcca gccagccgtt gtcgttgccg atgatgtaga
      841 gcgtcttctg gtccggcgag atcagcacgc cgttgcactt gccgcaatcg gtcgccaccc
      901 gcgttaccga cccgtcggga tcgagccggt aaaccgcgaa gccgtcctgg tagacaccct
      961 cgtggccgag gtagcgcgga tcgctgaaat agatcctacc cttctcgtcg atggtgatgt
     1021 cgttgagcgc gttgtaaggc cggccgttgt agagtccgga caggatgtag gtcttgccgg
     1081 tcttcatgtc ggtcttgacc agcatgcggc cgccgtagtc cgctcccagc gccgcgatca
     1141 tattgccatc acggtcgaac ttgatgccgt tcgacatgcc gctcggcgaa cggaagatcg
     1201 ttgtttcgcc gttcttcggg ttgtacttcc agatattgcc ggcctgcgcg tacttgcccg
     1261 aagcatcgcg gcagaacttg gtgaacgtga tgtcgctgaa atagatcatg ccgtcgtggc
     1321 cggccgccac accctcggtc agcatgcagc cgccatcgaa aatgagttcc agcttagcgc
     1381 ctgcgggaat gatgctcctg tcgccgaccg gtgccgggaa agtcgacggg tcggccgcat
     1441 gcaccgcggc ggaaaaagcc agcagcgtgc cgagaacggc ggcactccgt ttgaacagac
     1501 gcaaggtttc cattatctcc tccagttgtt atgcaagtgt cgaaacactc gattttggct
     1561 tcagacggga ccaataacac cacttcataa tccacctgcg cttgcagtgc tgcgatacca
     1621 ctgtagttta tatataaaat gggatttcaa atataaaaac ggggtgcctg cttgtcaata
     1681 cgaaaatcag ctgtaagaga gggggcgcgc tactccgccc ccgctctgga gaagggcctc
     1741 gacgtgctgg aactgctcgc ggccgagccc cacggcctga acctgcagga aatctcccgg
     1801 cggctgaagc gtacgtccaa cgaattgttt cgcatgctcg acgtgctggt gcggcgcggc
     1861 tatctggtgc gccagcccga ctctgcatac gtgctcaccc tgcggctgtt cgaactggca
     1921 caccggcatc ccggggtgga ccggctgctc gattgtgcaa tgccgcacat gcaggcgctc
     1981 gccggccgga ccggacaatc caatcacctg tgcgtccacc acgacgcgcg gctggtggtg
     2041 cttgcccagg cggaaccgcc ggaaccgatg tcctgctccg tgcggcaggg agcacatttt
     2101 gcgtttcacg atgaccgggt atcggcacgc gtgatcacgg cgttccagac gggcgagcag
     2161 cgcgatcagt acctgtccga ggtactcggc tcgcgtcgcg ccgcgccggc gcgcaggcgg
     2221 gcgctgatcg cacgcctgga agcgacccgc aggcgcggct atgacgaagg cccgagcgat
     2281 accatcaacg gtgtggtgga tatctgcttt cccatcttcg atcgcttcgg ggcgatcgcc
     2341 gcgctcaata tcgtctacct caggcagcgc gattcgcggg tcacgatccc ggcggcgagg
     2401 gagaaattgc gtcacacggc tcaggcgatc tcgagatcgc tcggctggct tggccgcgcg
     2461 aacggccggc ctggcgatgg gtaatgggta atgggtgtgg acacaaggaa gagtagaaac
     2521 ggcgaaaaat tgcaaacgca gtaaatatcc gtctgcgtca acagtcacgc attgcccatt
     2581 gcccattacc cattgcccat tgcccatt
//
LOCUS       DASQOP010000368         3842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10806
            k141_804792, whole genome shotgun sequence.
ACCESSION   DASQOP010000368 DASQOP010000000
VERSION     DASQOP010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946285
            Sequence Read Archive: SRR5882672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 10:05:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,808
            Genes (coding)                    :: 2,786
            CDSs (with protein)               :: 2,786
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 1 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804792"
                     /isolate="SMAG_U10806"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(304..759)
                     /locus_tag="VE030_14215"
     CDS             complement(304..759)
                     /locus_tag="VE030_14215"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HZF24607.1"
                     /translation="MTRMRFRRSLATLTVAFTLAACSDASPLPRLGADDVVLAFGDSI
                     TYGTGARPAESYPEVLAQLIGRRVVGAGVPGEVTANGLQRLPEVLDEVKPKILLLCLG
                     GNDMLRKVEPVAIEANLRTMVRIARDRGIAVVLIAVPKPALFGGNAAFY"
     gene            complement(961..2301)
                     /gene="pmbA"
                     /locus_tag="VE030_14220"
     CDS             complement(961..2301)
                     /gene="pmbA"
                     /locus_tag="VE030_14220"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HZF24608.1"
                     /translation="MPSTEFTHETVTLKQIASDVLEYARDRGATAAETEVSEGFGQGV
                     TVRRGEVETIEYNRDKGVGVSVYIGKQRGHASTSDFSRKALRDTVDAALSIARLTASD
                     EFSGLADAGLLATEFPDLDLYHPWSISVEEAIELARSSENAAFAADRLVSNSEGATVS
                     VQQSHFAYGNSHGFLAGYPSSRHSVMCAVIAGKDETMQRDDWYTTARAAADLEPAADV
                     GSRAGKRAARRVGARKVATVRVPVLFEAPIASSLLGHFVAAVSGGSLYRKSSFLLDSL
                     GKQVFSPIVNIHDLPHVRKGLASSPFDDEGVATRARDVVGSGVVEGYFLGSYSARKLG
                     MKSTGNAGGNHNLVMDSTGEDFAALLKKMGTGLLVTEMLGQGINMVTGDYSRGAAGFW
                     VENGEIAYPVQEVTVAGNLKDMFGNIVAVGTDVMVRGSRQTGSVLVERMTVAGE"
     gene            2422..2913
                     /gene="yjgA"
                     /locus_tag="VE030_14225"
     CDS             2422..2913
                     /gene="yjgA"
                     /locus_tag="VE030_14225"
                     /inference="COORDINATES: protein motif:HMM:NF016626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HZF24609.1"
                     /translation="MEEELEISKTQRKREMQALQNLGEELVALSPDKLAGLGLPTRLL
                     DAVLEAKRISKFGALRRQMQYVGRLMRDADADVIRSQLDVWKGTSVAETARLHAIERW
                     RTRLVEDELALGELLGQFPHADAQRLRALIRNVKREAAAKKPPKSFRELFHELRSIIL
                     RPD"
     gene            3067..3660
                     /gene="mog"
                     /locus_tag="VE030_14230"
     CDS             3067..3660
                     /gene="mog"
                     /locus_tag="VE030_14230"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HZF24610.1"
                     /translation="MNENEQVVIGLVSISDRASKGVYKDEGVPALEEWLGRVVLSPRR
                     IVTKLIPDERPVIEATLKELCDREGCHLVLTTGGTGPAPRDVTPEATLAVADKVMPGF
                     GEQMRQISLSFVPTAILSRQVGVIRGSTLIINLPGQPKSIKETLEGLKDADGRQKVHG
                     IFAAVPYCIDLIGGPYIETDETVVKAFRPKSAIRPKS"
BASE COUNT          744 a         1231 c         1168 g          699 t
ORIGIN      
        1 cacgcgcggc gggtgctctc tgccgccgcc gcaggcgcag acgaaaagaa gtccaagagc
       61 tgatatggct agacgtgtgc gcatgatctt ctccttcctg atatctcccg cgtccaaaat
      121 ggcattcccc tcaccgttga attaaaccgc gcccgtccgc cgcagcaacg ccgcgacggc
      181 ttccgccagg cgccggtagc ctttggaatt ggggtggatc ggatcggctt tgaattcgtt
      241 gtcgaacagg atttccctga gaatcctgct ctccagaggc agatcgtttt ccctaacgat
      301 tgtctaatag aaggcggcgt tgccgccgaa caatgccggt ttgggcacgg cgatcagcac
      361 gacggcgatg ccgcgatccc tggcgatgcg caccatcgtc cgcaagttgg cctcgatggc
      421 caccggctcg accttgcgca acatgtcgtt tccgcccagg caaagcaaca ggatcttcgg
      481 cttcacttcg tcgagcacct ccggcaggcg ctgcaggccg ttcgccgtaa cttctccggg
      541 gacaccggcg ccgaccacgc ggcggccgat caattgggcc agcacctccg ggtaactctc
      601 tgcggggcgt gccccggtcc cgtaggtgat gctgtcgccg aaagcgagca cgacgtcatc
      661 cgcgccaagc cgcggcagtg gcgaggcatc gctgcaagcg gcgagcgtga acgcgacggt
      721 tagagttgcc agcgacctcc ggaagcgcat cctggtcatg caacacgacc ggccgttgtc
      781 ccacgcgcca ggcacgaggc gcggggtgcg aggcacgagg aaattcgctt tgctactttc
      841 gcaagaagag gcattgcgcc cttacccgtt gaggaaccag tgtttcagca ttaccttcgt
      901 tcttcgcccc tatcgttgcc ctcgcgcctc gcgcccagag cctagtgcct cgcccctgct
      961 tcattccccc gccaccgtca ttctctcgac cagcacagag ccggtctggc gcgagccgcg
     1021 caccatcaca tcggtgccga ctgcgacgat gtttccgaac atgtccttga gattgccggc
     1081 gactgtgacc tcctgcacag gataggcgat ctcgccgttc tcgacccaga atccggccgc
     1141 accgcgcgag tagtctccgg tcaccatatt gatcccctgg cccagcattt ccgttaccag
     1201 gagacccgtt cccattttct tcaaaagcgc ggcgaaatcc tcgccggtcg aatccattac
     1261 caggttgtgg ttgccgcccg cattgccggt ggacttcatt cccagttttc gcgccgagta
     1321 gctgcctaga aaatagccct cgaccacacc gctcccgaca acgtcgcggg cgcgggtcgc
     1381 cacgccctcg tcgtcgaacg ggctgctggc gaggcctttg cgcacatgag gcaggtcatg
     1441 aatgttcacg attggcgaga acacctgctt ccccaggctg tcgagcagga acgaggattt
     1501 ccggtacagg ctgccgccgc tcaccgcggc gacgaaatgg cccagcaggc tcgatgcgat
     1561 cggcgcttcg aacagcaccg gcacgcgcac ggtggcaacc ttgcgcgcgc cgacccggcg
     1621 cgcggcacgc tttccggcgc ggctgccgac atcggccgcc ggttccagat cggcggctgc
     1681 gcgcgcggtc gtgtaccagt cgtcgcgctg catggtctcg tccttgcccg cgatcaccgc
     1741 gcacatgacg ctgtgccggg aactgggata gcccgccaga aagccgtggg aattgccgta
     1801 ggcgaaatgc gactgctgaa ccgaaaccgt ggcgccttca gagttgctga ccagacgatc
     1861 ggcggcgaac gcggcattct cggacgagcg cgccagctcg attgcctcct ccaccgaaat
     1921 cgaccacggg tgatacagat cgagatcggg aaattccgtc gccagcaggc cggcgtcggc
     1981 gagcccggaa aactcgtcgg aagcggtgag tcgcgcgatc gatagcgccg catcgaccgt
     2041 gtcgcgcagc gccttgcggg aaaagtcgga cgtgctggcg tggccacgct gcttgccgat
     2101 gtataccgag acgcccacgc ccttgtcgcg gttgtactcg atggtttcga cctcgccgcg
     2161 gcgcacggtc acgccctgcc cgaagccctc gctcacctcg gtctcggcgg cggtggcgcc
     2221 gcggtcgcgg gcatactcga ggacatcgct ggcaatctgc ttgagcgtga cggtttcgtg
     2281 ggtgaattcg gtgctgggca cgatgattcc ggttgtggca cgaacaaatg aacgcggcgt
     2341 gccgccatct ttcgagcggg gcggcacgcc gcaaatggaa ttttcgttat gataccagtt
     2401 gcgacggccc cgacttctgc gatggaagaa gaacttgaga tcagcaagac ccagcgcaag
     2461 cgcgagatgc aggcgctgca gaacctcggc gaagagctgg tcgccttgag cccggacaag
     2521 ctggccgggc tcggcctccc cacccggctt ctcgacgcgg tactggaagc caagcgcatc
     2581 tccaaattcg gcgcgctgcg gcgccagatg cagtacgtgg gccggctgat gcgcgatgcc
     2641 gacgccgacg tcatccgctc ccaactcgac gtctggaaag gcacctcggt cgcggagacc
     2701 gcgcgcctgc atgccataga gcgatggcga acgcggctgg tcgaggacga actggcgctg
     2761 ggcgagctgc tcggccagtt tccccacgcc gacgcgcagc gcctgcgcgc gctgatccgc
     2821 aacgtcaagc gcgaagccgc ggccaagaag ccgcccaaaa gcttccgcga actgtttcac
     2881 gagcttcgca gcatcatctt gaggccagac taaccacaga gacacagaga cacagagaac
     2941 gtcttgatca gaaaataaaa gcgaaaataa tgcgtttaat gatcatttgc ctccttaact
     3001 ttgtccatcg cagtttgtca ggttctgttt ttctctgtgc ctctgtgtct ctgtggtgaa
     3061 gaccctatga acgaaaacga acaagtggtc atcgggctgg tgtcgatcag cgaccgggca
     3121 tcgaaaggcg tgtacaagga cgaaggcgtt ccggcgctcg aagagtggct gggacgcgtc
     3181 gtgctcagtc cgcgccggat agtcacgaag ctgattccgg acgagcggcc ggtgatcgaa
     3241 gccacgctga aggagctatg cgacagggaa ggctgccatc tggtgctcac caccggcggc
     3301 accggtccgg cgccgcgcga cgtgacgccg gaggcaacgc tggcggttgc cgacaaagtg
     3361 atgccggggt tcggcgagca gatgcgccag atcagcctga gcttcgtgcc caccgcgatc
     3421 ctgtcgcggc aggtcggcgt cattcgcggg agcaccctca tcatcaacct gccgggccag
     3481 ccgaaatcca tcaaggaaac gctggaaggc ctgaaggacg ccgacggcag gcagaaggtg
     3541 cacggcatct tcgccgcggt gccttactgc atcgacctga tcggcgggcc ttacatcgag
     3601 accgacgaga ctgtggtcaa ggccttccgt cccaaatccg caattcgccc gaagagctaa
     3661 ccttatcacc gcaaagacgc aaagaacgcg aaggagtaca aacccaaaag agaagaagac
     3721 attcagaggt gagtaaaaga tgcggttgca caacgacaca atcaaccgta cgggtgtgtt
     3781 cgcttattcc ggtatccttc tgttccattg attttcttcg cgtcctttgc gtctttgcgg
     3841 tg
//
